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Estudo comparativo )0.7(entre )0.5(os procedimentos )]TJ +-0.024 Tw T* +[(manual e automatizado )0.8(na contagem diferencial )0.6(dos )-0.6(leuc\363citos. Rev Bras )]TJ +0 Tw T* +(Anal Clin. 2001;33\(1\):35-7.)Tj +/C2_0 1 Tf +-1.772 -1.604 Td +<001400130011>Tj +/Span<>> BDC +<0003>Tj +EMC +1.772 0 Td +[<002F0048005A004C00560003>-28.4<00360030000F0003>-28.7<00250044004C00510003>-28.4<0025002D000F0003>-28.5<002500440057004800560003>-28<002C00110003>-28.4<002B0048005000440057>0.5<0052004F0052004A004C00440003>-27.9<0053005500690057004C004600440003>-27.6<004700480003>-28.2<002700440046004C00480003>-27.9<00480003>-28.2<002F0048005A004C005600110003>-28.2<001C0057004B0003>]TJ +/TT0 1 Tf +-0.055 Tw T* +[(ed. )-54.8(Porto Alegre: Artmed; )-54.5(2006. )]TJ +0.172 Tw -1.772 -1.604 Td +[(1)36.6(1.)]TJ +/Span<>> BDC +( )Tj +EMC +1.772 0 Td +[(Automated )1.1(Hematology )0.7(Slide )0.6(Preparation )0.7(Unit SP-1000i. Instructions )]TJ +0 Tw T* +(for use. Kobe, Japan: Sysmex Corporation; 2009.)Tj +0.171 Tw -1.772 -1.604 Td +(12.)Tj +/Span<>> BDC +( )Tj +EMC +1.772 0 Td +[(Y)99.4(amamoto )18.2(T)73.9(, )17.4(T)69.9(abe )36.7(Y)128.8(, Ishii K, Itoh S, Maeno I, Matsumoto K, et al. )]TJ +0.281 Tw T* +[([Performance )0.5(evalu)0.5(ation of the CellaV)60.1(ision DM96 system in )17.7(WBC )]TJ +0 Tw T* +[(dif)17.7(ferentials]. Rinsho Byori. 2010;58\(9\):884-90. Japanese. )]TJ +/C2_0 1 Tf +-1.772 -1.604 Td +<001400160011>Tj +/Span<>> BDC +<0003>Tj +EMC +1.772 0 Td +[<0025004C004F004F0044005500470003>-13.4<0030000F0003>-14.3<002F0044004C0051>0.5<0048005C0003>-13.9<0028000F0003>40.8<00240055005000520052004A005800500003>-13.9<0033>110.7<000F0003>40.6<0024004F0045004800550057004C0003>-13.6<0026000F0003>-14.1<0029004800510051004800570048004400580003>-13.4<0032000F0003>-14.3<002700440003>-14.1<002600520056005700440003>-13.9<002F00110003>]TJ +/TT0 1 Tf +0.081 Tw T* +[(Evaluation )0.7(of the CellaV)60(ision DM )-0.6(automated )0.7(microscope in pediatrics. )]TJ +/C2_0 1 Tf +0 Tw T* +<002C005100570003002D0003002F004400450003002B00480050004400570052004F001100030015001300140013001E00160015000B0018000C001D0018001600130010001B0011>Tj +-1.772 -1.604 Td +<001400170011>Tj +/Span<>> BDC +<0003>Tj +EMC +1.772 0 Td +[<00250055004C004A004A00560003>-78.4<0026000F0003>-78.4<002F00520051004A0044004C00550003>-78.1<002C000F0003>-78.4<0036004F00440059004C004E0003>-78.1<0030000F0003>-61<0037004B005A0044004C005700480003>-77.8<002E000F0003>-78.6<0030004C004F004F00560003>-78.2<0035000F0003>-60.8<0037004B00440059004400550044004D00440003>-60<0039>128.8<000F0003>-78.4<004800570003>-78.1<0044004F00110003>]TJ +T* +[<0026004400510003>-92.2<0044005800570052005000440057004800470003>-91.8<0045004F0052005200470003>-92.3<00C0004F00500003>-92.2<004400510044004F005C0056004C00560003>-92<005500480053004F0044004600480003>-91.9<0057004B00480003>-92.1<00500044005100580044004F0003>-91.6<0047004C0049>17.7<004900480055004800510057004C0044004F00220003>-36.5<002400510003>]TJ +/TT0 1 Tf +0.081 Tw T* +[(evaluation )0.9(of the CellaV)59.9(ision DM96 automated )0.9(image )0.6(analysis system. )]TJ +/C2_0 1 Tf +0 Tw T* +<002C005100570003002D0003002F004400450003002B00480050004400570052004F00110003001500130013001C001E00160014000B0014000C001D0017001B0010001900130011>Tj +/TT0 1 Tf +-0.005 Tc 0.1 Tw -1.772 -1.604 Td +(15.)Tj +/Span<>> BDC +( )Tj +EMC +1.772 0 Td +[(Cornet E, Perol JP)110.7(, )17.4(T)34.9(roussard X. Performance evaluation and relevance)-4.9( )]TJ +0.05 Tw T* +[(of the CellaV)59.4(ision DM96 system in routine analysis and in patients with)-5( )]TJ +/C2_0 1 Tf +0.005 Tw T* +[<00500044004F004C004A00510044005100570003>12.6<004B00480050004400570052004F0052004A004C00460044004F0003>12.6<0047004C00560048004400560048005600110003>12.5<002C005100570003>12.6<002D0003>12.6<002F004400450003>12.5<002B00480050004400570052004F00110003>12.6<001500130013001B001E00160013000B0019000C001D0018001600190010001700150011>]TJ +/TT0 1 Tf +0 Tc 0.431 Tw -1.772 -1.604 Td +(16.)Tj +/Span<>> BDC +( )Tj +EMC +1.772 0 Td +[(Surcouf C, Delaune D, Samson )17.4(T)73.9(, Foissaud )17.5(V)128.7(. [Automated )0.5(cell )]TJ +0.145 Tw T* +[(recognition )0.6(in hematology: )0.8(CellaV)60(ision DM96 )17.1(TM system]. )54.6(Ann Biol )]TJ +0 Tw T* +(Clin \(Paris\). 2009;67\(4\):419-24. French.)Tj +0.166 Tw -1.772 -1.604 Td +(17.)Tj +/Span<>> BDC +( )Tj +EMC +1.772 0 Td +[(Gao )37.1(Y)128.8(, Mansoor )55(A, )17.7(W)79.8(ood B, Nelson H, Higa D, Naugler )0.6(C. Platelet )]TJ +0.078 Tw T* +[(count )0.5(estimation )1.2(using the )0.6(CellaV)60(ision DM96 system. 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Zog 不U~̆ uJ7T?H Y?F޺`ht>p e+d骙r,e5e7K!t/ꏴRAs /nrNYyUGUB@f"-&N 'n!ɘȘȘȘ2c&2fNn.mx`MX4B3#/cM2sErY^W{]R2˔ O0&;EIs"P@u"P@m"0I2HsJݹRw.LT.LT.LT.LT.LT.~ S0 SН+uwI@;'bd3MJ> +.EC_@o~Sg}l11LJt DvYhRS,`8s+35I2K)$4+nܬE}9VoSM +>(KRm:a﮵'~Uat$9r_AEgh'*R`2!s׉"+SE,둠B/ y+ۦ@;P t)Хc0B?m!Ris*mNXP6 le(@w leJS9"T)ۧO,=.tpg + q9@juA١4: +!~AM^YZͱ/}tV +[~+pෂs䲟RIll>&Il>&᳔8O R)Z<6 " +\+JV +Rh@+Z)JV +>LV +R.苐 }>!0 6Т2CТ2 -*CТ2 -*C2玾H2]e%jc &~ƚkkM_o.߼]9߻uιfN^4ۻjۣs)a@}|9 >anWyM ƀB~X0qT5|Y|Y`_,e/A/+<7v<] +(DO^3O#3xSwUe.(tA +]PB.(tA +]rG_$D('*SGb g ku哾rbT{ιvc s 7jG[4?ث{o?gδrZ(WUo样:;a턹x`N<~&i+g0Xs5SƝ֮{LlH)CqsjpN Ω f1@b3 fI f1q4|: Hqʡ3IWE;.)K$RT1%\TEYV*E;~,MI7ޚ$u½RTYuw@<V* +1yAbA6- p +ȦdiٴlZٴlZi.н#t,U5vo}n+d8ےt?(~GOϰ-ɝHމHމHމD)&kI<oeM}&g[|6Zfywb&Sow/5R3VӐyx?s!(w?V6o}w&v<0ck_sD[Ӌɟ7Oǣ P(O>?bZ.t;zŶ8*XXTQ>HԹ,L.g+O皵lE/x['t1Oı-9@?N 4D7}WE/|> + gۋ5 2?D+K^doDy?Q-1w1Q2LvOb +‹ӈ)u+ܟ69Mc[2PE_.`mQX +rks%!jsQ)n\kdeMs>mMk\Z{ZUYS"D&>L-tD,GrO-WWL㳴I7MO!sY 73|9F +*aLtRè8F⓸S4>s E| _Wq'#Jon|[wp~|>~AaÏ2MuRm"ke佌cAK$î@|aܻXYMrg{QQ=aZe/qѻRes_L]{ո d endstream endobj 30 0 obj <>stream +q +/GS0 gs +/Fm0 Do +Q +BT +0 0 0 1 k +/GS1 gs +/TT0 1 Tf +7 0 0 7 36.8504 31.0197 Tm +(406)Tj +ET +q +0 g +/GS0 gs +/Fm1 Do +Q +BT +/TT0 1 Tf +0.02 Tc 0.204 Tw 9.5 0 0 9.5 325.9843 367.8482 Tm +[(The present study demonstrates the validation process)19.8( )]TJ +0.121 Tw -2.089 -1.211 Td +(for the implementation of the Sysmex)Tj +0 Tc 0 Tw 5.5385 0 0 5.5385 462.5032 359.5117 Tm +(\256)Tj +0.02 Tc 0.121 Tw 9.5 0 0 9.5 466.8219 356.3482 Tm +[( SP-1000i equipment)20.2( )]TJ +0.112 Tw -16.914 -1.211 Td +[(in the laboratory routine, where the quality of blood smears)19.9( )]TJ +0.327 Tw 0 -1.211 TD +[(and slide staining were evaluated using the CellaV)59.3(ision)]TJ +0 Tc 0 Tw 5.5385 0 0 5.5385 548.548 336.5117 Tm +(\256)Tj +9.5 0 0 9.5 552.7559 333.3482 Tm +( )Tj +0.02 Tc 0.043 Tw -25.959 -1.211 Td +[(DM96 equipment which had previously been validated in the)20.2( )]TJ +0 Tw T* +[(laboratory routine.)20( )]TJ +0 Tc 0.304 Tw 2.089 -1.211 Td +(The preparation and staining of the blood smear on a )Tj +/C2_0 1 Tf +0 Tw -2.089 -1.211 Td +[<0050004C004600550052005600460052005300480003>-162<0056004F004C004700480003>-162.6<0044005500480003>-162.3<0049005800510047004400500048005100570044004F0003>-161.6<005600570048005300560003>-162.6<005700520003>-162.4<00530048005500490052005500500003>-162.3<00440003>-162.5<005400580044004F004C00C0004800470003>]TJ +/TT0 1 Tf +0.231 Tw T* +[(microscopic )0.9(examination, )1.3(however the manual )0.8(preparation )0.9(of )]TJ +0.079 Tw 0 -1.211 TD +[(CBC slides, despite )0.6(being )0.5(traditional )1.3(and satisfying, has certain )]TJ +/C2_0 1 Tf +0 Tw 0 -1.211 TD +[<004F004C0050004C005700440057004C0052005100560003>-94.2<0057004B004400570003>-94.9<0046004400510003>-95.1<0047004C0055004800460057004F005C0003>-94.6<004C005100C1005800480051004600480003>-94.9<0057004B00480003>-95.1<0047004C0049>17.8<004900480055004800510057004C0044004F0003>-94.5<004600520058005100570003>-95<0044005100470003>]TJ +/TT0 1 Tf +0 -1.211 TD +(morphological analysis of cells. )Tj +/C2_0 1 Tf +2.089 -1.211 Td +[<0037004B00480003>-50.6<004C004700480044>0.5<004F0003>-50.7<0045004F0052005200470003>-50.8<005600500048004400550003>-50.5<0049005200550003>-50.9<00440003>-50.7<005400580044004F004C00C0004800470003>-50.9<0026002500260003>-50.8<004400510044004F005C0056004C00560003>-50.6<0056004B00520058004F00470003>]TJ +-2.089 -1.211 Td +[<004500480003>-4.8<00580051004C0049005200550050000F0003>-4.7<00490055004800480003>-4.6<005200490003>-5<0045005800450045004F004800560003>-4.8<0044005100470003>-4.9<00C10044005A00560003>-5.3<0044005100470003>-4.8<005A004C0057004B0003>-4.8<005A0048004F004F0010005600570044004C0051004800470003>-4.2<00460048004F004F00560003>]TJ +T* +[<0057004B004400570003>-132.4<0048005100440045004F00480003>-132.4<0057004B0048004C00550003>-132.4<00460052005500550048004600570003>-132.4<004C0047004800510057004C00C0004600440057004C005200510003>-131.8<0044005100470003>-132.7<0046004F004400560056004C00C0004600440057004C0052005100110003>-114.6<0037004B0048005600480003>]TJ +/TT0 1 Tf +-0.003 Tw T* +[(requirements )0.8(in the preparation )0.8(of a blood smear are achieved )0.8(by )]TJ +0 Tw 0 -1.211 TD +(automated equipment. )Tj +-0.095 Tw 2.089 -1.211 Td +(The Sysmex)Tj +0 Tw 5.5385 0 0 5.5385 372.3169 175.5117 Tm +(\256)Tj +-0.095 Tw 9.5 0 0 9.5 376.5248 172.3482 Tm +[( SP-1000i equipm)0.5(ent automates the preparation )]TJ +0.197 Tw -7.409 -1.211 Td +[(and staining of slides to standardize )1(the )0.5(preparation )0.9(of blood )]TJ +0.27 Tw T* +[(smears using the hematocrit )1(value of the sample, regulating )]TJ +0.093 Tw 0 -1.211 TD +[(the )0.5(amount of blood, the velocity )1(and the )0.5(angle )0.6(to improve )0.6(the )]TJ +0.209 Tw T* +[(quality )0.9(of the )0.5(smear)55(. In this study the )0.6(smear )0.5(and staining )0.7(of )]TJ +0.102 Tw 0 -1.211 TD +(slides prepared in the Sysmex)Tj +0 Tw 5.5385 0 0 5.5385 423.4652 118.0117 Tm +(\256)Tj +0.102 Tw 9.5 0 0 9.5 427.6732 114.8482 Tm +( SP-1000i proved to be of good )Tj +0.04 Tw -12.793 -1.211 Td +[(quality )0.7(as all )0.5(of the slides provided acceptable )1.2(results in the cell )]TJ +-0.004 Tw T* +[(localization )1.4(and artifacts )0.7(tests when processed for quality )0.7(control )]TJ +0 Tw 0 -1.211 TD +[(in the CellaV)60.5(ision)]TJ +5.5385 0 0 5.5385 374.7059 83.5117 Tm +(\256)Tj +9.5 0 0 9.5 378.9139 80.3482 Tm +( DM96 equipment. )Tj +/C2_0 1 Tf +-5.572 -1.211 Td +[<0037004B00480003>34.9<00530055004800100046004F004400560056004C00C000460044>0.5<0057004C005200510003>34.9<005200490003>34.6<004F00480058004E00520046005C00570048>0.8<0003>34.6<0047004C0049>17.7<004900480055004800510057004C0044004F0003>35.6<0046005200580051005700560003>34.7<0045005C0003>34.6<0057004B00480003>]TJ +/TT0 1 Tf +-2.089 -1.211 Td +[(CellaV)60.1(ision)]TJ +5.5385 0 0 5.5385 350.9606 60.5117 Tm +(\256)Tj +0.059 Tw 9.5 0 0 9.5 355.1685 57.3482 Tm +[( DM96 device )0.6(showed that the cells were correctly )]TJ +ET +0 0 0 0.1 k +/GS0 gs +95.244 690.701 399.685 22 re +f +BT +0 0 0 1 k +/GS1 gs +/TT0 1 Tf +0.01 Tc 0.269 Tw 8 0 0 8 98.0787 704.2008 Tm +[(T)70(able 2 - Evaluation of the quality of slides produced by the Sysmex)]TJ +0 Tc 0 Tw 4.664 0 0 4.664 348.7637 706.8648 Tm +(\256)Tj +0.01 Tc 0.269 Tw 8 0 0 8 352.3538 704.2008 Tm +( SP-1000i, using quality control by the)Tj +0 Tc 0 Tw 17.468 0 Td +( )Tj +0.01 Tc -0.01 Tw -49.252 -1.125 Td +[(CellaV)59.4(ision)]TJ +0 Tc 0 Tw 4.664 0 0 4.664 136.7009 697.8648 Tm +(\256)Tj +0.01 Tc 8 0 0 8 140.291 695.2008 Tm +( DM96 for each hematocrit level)Tj +ET +0 0 0 0.1 k +/GS0 gs +95.244 541.348 399.685 22 re +f +BT +0 0 0 1 k +/GS1 gs +/C2_0 1 Tf +-0.01 Tw 8 0 0 8 98.0787 554.8482 Tm +[<0037>70<00440045004F00480003>-7.5<00160003>-7.6<00100003>-7.5<0031005800500045004800550003>-7.6<005200490003>-7.6<0056004F004C0047004800560003>-7.6<0044005100470003>-7.6<004F00480058004E00520046005C0057004800560003>-7.6<004C0050005300550052005300480055004F005C0003>-7.5<0046004F004400560056004C00C0004800470003>-7.5<0045005C0003>-7.6<0057004B00480003>-7.5<00260048004F004F00440039>59.4<004C0056004C00520051>]TJ +/TT0 1 Tf +0 Tc 0 Tw 4.664 0 0 4.664 371.2989 557.5122 Tm +(\256)Tj +0.01 Tc 0.008 Tw 8 0 0 8 374.8891 554.8482 Tm +[( DM96 using )0.5(slides prepared )0.5(by the)10.1( )]TJ +-0.01 Tw -34.601 -1.125 Td +(Sysmex)Tj +0 Tc 0 Tw 4.664 0 0 4.664 123.8947 548.5122 Tm +(\256)Tj +0.01 Tc 8 0 0 8 127.4848 545.8482 Tm +( SP-1000i)Tj +ET +0 0 0 1 K +0.25 w +/GS2 gs +q 1 0 0 1 137.7637 580.9278 cm +0 0 m +5.669 0 l +S +Q +q 1 0 0 1 143.433 580.9278 cm +0 0 m +51.024 0 l +S +Q +q 1 0 0 1 194.4566 580.9278 cm +0 0 m +5.669 0 l +S +Q +q 1 0 0 1 200.1259 580.9278 cm +0 0 m +65.197 0 l +S +Q +q 1 0 0 1 265.3228 580.9278 cm +0 0 m +5.669 0 l +S +Q +q 1 0 0 1 270.9921 580.9278 cm +0 0 m +70.866 0 l +S +Q +q 1 0 0 1 341.8582 580.9278 cm +0 0 m +5.669 0 l +S +Q +q 1 0 0 1 347.5275 580.9278 cm +0 0 m +70.866 0 l +S +Q +q 1 0 0 1 418.3936 580.9278 cm +0 0 m +5.669 0 l +S +Q +q 1 0 0 1 424.063 580.9278 cm +0 0 m +70.866 0 l +S +Q +q 1 0 0 1 95.244 690.5764 cm +0 0 m +42.52 0 l +S +Q +q 1 0 0 1 137.7637 690.5764 cm +0 0 m +5.669 0 l +S +Q +q 1 0 0 1 143.433 690.5764 cm +0 0 m +51.024 0 l +S +Q +q 1 0 0 1 194.4566 690.5764 cm +0 0 m +5.669 0 l +S +Q +q 1 0 0 1 200.1259 690.5764 cm +0 0 m +65.197 0 l +S +Q +q 1 0 0 1 265.3228 690.5764 cm +0 0 m +5.669 0 l +S +Q +q 1 0 0 1 270.9921 690.5764 cm +0 0 m +70.866 0 l +S +Q +q 1 0 0 1 341.8582 690.5764 cm +0 0 m +5.669 0 l +S +Q +q 1 0 0 1 347.5275 690.5764 cm +0 0 m +70.866 0 l +S +Q +q 1 0 0 1 418.3936 690.5764 cm +0 0 m +5.669 0 l +S +Q +q 1 0 0 1 424.063 690.5764 cm +0 0 m +70.866 0 l +S +Q +q 1 0 0 1 95.244 671.2821 cm +0 0 m +42.52 0 l +S +Q +q 1 0 0 1 137.7637 671.2821 cm +0 0 m +5.669 0 l +S +Q +q 1 0 0 1 143.433 671.2821 cm +0 0 m +51.024 0 l +S +Q +q 1 0 0 1 194.4566 671.2821 cm +0 0 m +5.669 0 l +S +Q +q 1 0 0 1 200.1259 671.2821 cm +0 0 m +65.197 0 l +S +Q +q 1 0 0 1 265.3228 671.2821 cm +0 0 m +5.669 0 l +S +Q +q 1 0 0 1 270.9921 671.2821 cm +0 0 m +70.866 0 l +S +Q +q 1 0 0 1 341.8582 671.2821 cm +0 0 m +5.669 0 l +S +Q +q 1 0 0 1 347.5275 671.2821 cm +0 0 m +70.866 0 l +S +Q +q 1 0 0 1 418.3936 671.2821 cm +0 0 m +5.669 0 l +S +Q +q 1 0 0 1 424.063 671.2821 cm +0 0 m +70.866 0 l +S +Q +q 1 0 0 1 95.244 580.9278 cm +0 0 m +42.52 0 l +S +Q +BT +/GS1 gs +/TT1 1 Tf +0 Tc 7.5 0 0 7.5 166.8593 678.1168 Tm +(n)Tj +5.381 0 Td +(Hematocrit \(%\))Tj +9.686 0.533 Td +(Leukocyte count )Tj +1.552 -1.067 Td +(\(x10)Tj +4.3725 0 0 4.3725 305.2533 676.6143 Tm +(3)Tj +7.5 0 0 7.5 307.4395 674.1168 Tm +(/\265L\))Tj +6.445 1.067 Td +(Cell Localization )Tj +1.796 -1.067 Td +[(T)91.8(est \(%\))]TJ +9.187 0.533 Td +(Artifacts \(%\))Tj +/C2_0 1 Tf +-45.343 -2.039 Td +<002F004800590048004F00030014>Tj +/TT0 1 Tf +8.949 0 Td +(10)Tj +6.48 0 Td +(16.68 \261 1.87)Tj +10.577 0 Td +(9.9 \261 1.3)Tj +9.455 0 Td +(99.85 \261 0.24)Tj +9.955 0 Td +(15.24 \261 5.53)Tj +0 0 0 0 k +/GS0 gs +(0)Tj +0 0 0 1 k +/GS1 gs +/C2_0 1 Tf +-45.415 -1.506 Td +<002F004800590048004F00030015>Tj +/TT0 1 Tf +8.949 0 Td +(10)Tj +6.48 0 Td +(22.96 \261 1.13)Tj +10.077 0 Td +(10.1 \261 2.1)Tj +0 0 0 0 k +/GS0 gs +(0)Tj +0 0 0 1 k +/GS1 gs +11.729 0 Td +(100)Tj +8.199 0 Td +[(15.72 \261 1)36.8(1.42)]TJ +/C2_0 1 Tf +-45.433 -1.506 Td +<002F004800590048004F00030016>Tj +/TT0 1 Tf +8.949 0 Td +(10)Tj +6.48 0 Td +(26.96 \261 1.39)Tj +10.577 0 Td +(9.4 \261 1.5)Tj +9.455 0 Td +(99.85 \261 0.24)Tj +0 0 0 0 k +/GS0 gs +10.33 0 Td +(000)Tj +0 0 0 1 k +/GS1 gs +(6 \261 3.68)Tj +/C2_0 1 Tf +-45.79 -1.506 Td +<002F004800590048004F00030017>Tj +/TT0 1 Tf +8.949 0 Td +(10)Tj +6.48 0 Td +(32.49 \261 1.38)Tj +10.577 0 Td +(9.6 \261 1.8)Tj +9.455 0 Td +(99.81 \261 0.32)Tj +9.973 0 Td +[(13.25 \261 1)36.8(1.21)]TJ +/C2_0 1 Tf +-45.433 -1.506 Td +<002F004800590048004F00030018>Tj +/TT0 1 Tf +8.949 0 Td +(10)Tj +0 0 0 0 k +/GS0 gs +6.48 0 Td +(0)Tj +0 0 0 1 k +/GS1 gs +(37.2 \261 1.47)Tj +10.577 0 Td +(8.7 \261 1.5)Tj +9.705 0 Td +(99.9 \261 0.21)Tj +10.205 0 Td +(8.35 \261 4.81)Tj +/C2_0 1 Tf +-45.915 -1.506 Td +<002F004800590048004F00030019>Tj +/TT0 1 Tf +8.949 0 Td +(10)Tj +6.48 0 Td +(41.16 \261 0.78)Tj +10.577 0 Td +(9.6 \261 1.1)Tj +11.229 0 Td +(100)Tj +8.18 0 Td +(10.67 \261 6.15)Tj +0 0 0 0 k +/GS0 gs +(0)Tj +0 0 0 1 k +/GS1 gs +/C2_0 1 Tf +-45.415 -1.506 Td +<002F004800590048004F0003001A>Tj +/TT0 1 Tf +8.949 0 Td +(10)Tj +6.48 0 Td +(46.44 \261 1.12)Tj +10.577 0 Td +(9.1 \261 1.4)Tj +11.229 0 Td +(100)Tj +8.18 0 Td +(15.64 \261 13.62)Tj +/C2_0 1 Tf +-45.415 -1.506 Td +<002F004800590048004F0003001B>Tj +/TT0 1 Tf +8.949 0 Td +(10)Tj +6.48 0 Td +(52.27 \261 1.95)Tj +10.577 0 Td +(9.5 \261 1.5)Tj +11.229 0 Td +(100)Tj +8.68 0 Td +(14.2 \261 7.75)Tj +ET +/GS2 gs +q 1 0 0 1 137.7637 400.9866 cm +0 0 m +5.669 0 l +S +Q +q 1 0 0 1 143.433 400.9866 cm +0 0 m +51.024 0 l +S +Q +q 1 0 0 1 194.4566 400.9866 cm +0 0 m +5.669 0 l +S +Q +q 1 0 0 1 200.1259 400.9866 cm +0 0 m +65.197 0 l +S +Q +q 1 0 0 1 265.3228 400.9866 cm +0 0 m +5.669 0 l +S +Q +q 1 0 0 1 270.9921 400.9866 cm +0 0 m +70.866 0 l +S +Q +q 1 0 0 1 341.8582 400.9866 cm +0 0 m +5.669 0 l +S +Q +q 1 0 0 1 347.5275 400.9866 cm +0 0 m +70.866 0 l +S +Q +q 1 0 0 1 418.3936 400.9866 cm +0 0 m +5.669 0 l +S +Q +q 1 0 0 1 424.063 400.9866 cm +0 0 m +70.866 0 l +S +Q +q 1 0 0 1 95.244 541.2238 cm +0 0 m +42.52 0 l +S +Q +q 1 0 0 1 137.7637 541.2238 cm +0 0 m +5.669 0 l +S +Q +q 1 0 0 1 143.433 541.2238 cm +0 0 m +51.024 0 l +S +Q +q 1 0 0 1 194.4566 541.2238 cm +0 0 m +5.669 0 l +S +Q +q 1 0 0 1 200.1259 541.2238 cm +0 0 m +65.197 0 l +S +Q +q 1 0 0 1 265.3228 541.2238 cm +0 0 m +5.669 0 l +S +Q +q 1 0 0 1 270.9921 541.2238 cm +0 0 m +70.866 0 l +S +Q +q 1 0 0 1 341.8582 541.2238 cm +0 0 m +5.669 0 l +S +Q +q 1 0 0 1 347.5275 541.2238 cm +0 0 m +70.866 0 l +S +Q +q 1 0 0 1 418.3936 541.2238 cm +0 0 m +5.669 0 l +S +Q +q 1 0 0 1 424.063 541.2238 cm +0 0 m +70.866 0 l +S +Q +q 1 0 0 1 265.3228 521.9295 cm +0 0 m +5.669 0 l +S +Q +q 1 0 0 1 270.9921 521.9295 cm +0 0 m +70.866 0 l +S +Q +q 1 0 0 1 341.8582 521.9295 cm +0 0 m +5.669 0 l +S +Q +q 1 0 0 1 347.5275 521.9295 cm +0 0 m +70.866 0 l +S +Q +q 1 0 0 1 418.3936 521.9295 cm +0 0 m +5.669 0 l +S +Q +q 1 0 0 1 424.063 521.9295 cm +0 0 m +70.866 0 l +S +Q +q 1 0 0 1 95.244 400.9866 cm +0 0 m +42.52 0 l +S +Q +q 1 0 0 1 95.244 502.6352 cm +0 0 m +42.52 0 l +S +Q +q 1 0 0 1 137.7637 502.6352 cm +0 0 m +5.669 0 l +S +Q +q 1 0 0 1 143.433 502.6352 cm +0 0 m +51.024 0 l +S +Q +q 1 0 0 1 194.4566 502.6352 cm +0 0 m +5.669 0 l +S +Q +q 1 0 0 1 200.1259 502.6352 cm +0 0 m +65.197 0 l +S +Q +q 1 0 0 1 265.3228 502.6352 cm +0 0 m +5.669 0 l +S +Q +q 1 0 0 1 270.9921 502.6352 cm +0 0 m +70.866 0 l +S +Q +q 1 0 0 1 341.8582 502.6352 cm +0 0 m +5.669 0 l +S +Q +q 1 0 0 1 347.5275 502.6352 cm +0 0 m +70.866 0 l +S +Q +q 1 0 0 1 418.3936 502.6352 cm +0 0 m +5.669 0 l +S +Q +q 1 0 0 1 424.063 502.6352 cm +0 0 m +70.866 0 l +S +Q +BT +/GS1 gs +/TT1 1 Tf +7.5 0 0 7.5 98.0787 532.7641 Tm +(Hematocrit )Tj +0 -1.067 TD +(level)Tj +6.935 0.533 Td +(Samples \(n\))Tj +7.309 0.533 Td +(Inadequate slides )Tj +3.098 -1.067 Td +(\(n\))Tj +15.878 0.533 Td +(Inadequate leukocytes)Tj +-9.141 -2.573 Td +[(Band neutr)17.3(ophils)]TJ +11.573 0.533 Td +(Segmented )Tj +-0.103 -1.067 Td +[(neutr)17.6(ophils)]TJ +10.336 0.533 Td +(Monocytes)Tj +/C2_0 1 Tf +-45.884 -2.039 Td +<002F004800590048004F00030014>Tj +/TT0 1 Tf +8.949 0 Td +(10)Tj +8.815 0 Td +(0)Tj +9.449 0 Td +(0)Tj +10.205 0 Td +(0)Tj +10.205 0 Td +(0)Tj +/C2_0 1 Tf +-47.622 -1.506 Td +<002F004800590048004F00030015>Tj +/TT0 1 Tf +8.949 0 Td +(10)Tj +8.815 0 Td +(1)Tj +9.449 0 Td +(1)Tj +10.205 0 Td +(0)Tj +10.205 0 Td +(0)Tj +/C2_0 1 Tf +-47.622 -1.506 Td 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3{w|2L5ߜ!Icxw=>)Kf{w/uGsݚtw/wr~43\AO\IݶxlzS[wƅ]R4cu[0?4uH gx\ = /Hʜ8'G|of =vúu-ZԵkL ’-XYXWPWXWPWdYAVAtLOS)Y,t8 +z졢JgYtV8+JgYTx*=""W[ns 3%8wFKq>stream +Hy|ǟggB2 DpP +YI+iM-Q-Z*Ղ9Br$,7!Ǜ]D;>4!ܳ33?L'DGLO#&;@1 -\; @G3N] +Pw='Izf޴ ܔ6*G(:m*,8X % +VC"`#la3lo`+l vC +Gcp0q8pY8\ Ȅ,@.te5P7nAC ܆ReP k Ut`SlOc[ v+]p|@/0q,F_p2Sq~sc)ױ*He\T2L̇a̟,=gY ֆcY^b6dl>}氏\6ű,b!v+q}x-^ᾼ.x["2ʇ|GW|4y|>/Fon "U^bJZlV]=8$8#.渿 8pcp'{/9ލ{,D$KP)B r|%@"7d!qSZ0U8,m SX+QyX 9b$7o68y:2¯R2/*(&Mb8(1qBgEpKPRFPy9NW7RSQzVT]^ c?ֈ8~Vr1(RY,20EAylyI[Ҕ d@'g(tw+t\NG^Ke\L.7%,L)rerԇ(?6;],o"WSHDB9F@[zk wM#)͔pL] `%^N2-a)^G. fdamFzs$o>N5י_"3b.6~?B`6fwhf;| c p.$o_4nd㠜nFQf*Fqw2&A. 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TD +[<001500130014>36.6<001400110003>-167.3<0037004B00480003>-184.9<0056004400500053004F004800560003>-184.9<005A0048005500480003>-184.9<00460052004F004F004800460057004800470003>-184.9<0044005100470003>-184.9<0056005700520055004800470003>-184.9<004C00510003>-184.9<001700100050002F>36.8<0003>-184.9<00570058004500480056>9.8<0003>]TJ +/TT0 1 Tf +0.14 Tw 0 -1.211 TD +[(with ethylenediaminetetraacetic acid \(EDT)79.8(A\)-K3 \(V)110.7(acuette )]TJ +0 Tc 0 Tw 5.5385 0 0 5.5385 282.4955 417.0117 Tm +(\256)Tj +9.5 0 0 9.5 286.7588 413.8482 Tm +(, )Tj +0.01 Tc -25.709 -1.211 Td +(Greiner bio-one\).)Tj +0 Tc -0.022 Tw 2.089 -1.211 Td +[(This )0.6(study )0.5(was approved )0.6(by the )0.8(Research )0.9(Ethics )0.9(Committee )]TJ +0 Tw -2.089 -1.211 Td +[(of ISCMP)91.3(A)54.5( \(no. 008939/201)36.8(1\).)]TJ +/TT2 1 Tf +10 0 0 10 62.3622 356.3482 Tm +[(Prepar)12(ation of Blood Smears)]TJ +/TT0 1 Tf +0.137 Tw 9.5 0 0 9.5 62.3622 333.3482 Tm +[(The )0.6(Sysmex)]TJ +0 Tw 5.5385 0 0 5.5385 110.892 336.5117 Tm +(\256)Tj +0.137 Tw 9.5 0 0 9.5 115.0999 333.3482 Tm +[( SP-1000i equipme)0.6(nt )0.5(prepares )0.5(blood smears )]TJ +0.077 Tw -7.64 -1.211 Td +[(according )0.9(to the )0.5(hematocrit )1.4(value of each )0.7(sample, )0.7(adjusting )0.8(the )]TJ +-0.012 Tw T* +[(level )0.6(of the blood smear by the angle of inclination )1.1(and the speed )]TJ +0.077 Tw 0 -1.211 TD +[(of the )0.5(spreader )0.7(slide )0.7(\(T)70(able )0.8(1\))]TJ +0 Tw 5.5385 0 0 5.5385 159.7139 302.0117 Tm +[(\(1)36.6(1\))]TJ +0.077 Tw 9.5 0 0 9.5 168.7383 298.8482 Tm +[(. )17.9(T)69.9(en )0.5(samples )0.6(for each )0.8(level )0.9(of )]TJ +0 Tw -13.286 -1.211 Td +(blood smear performed by the equipment were selected.)Tj +0.01 Tc 0.009 Tw 2.089 -1.211 Td +(For each patient two slides were prepared by the Sysmex)Tj +0 Tc 0 Tw 5.5385 0 0 5.5385 284.9259 279.0117 Tm +(\256)Tj +9.5 0 0 9.5 289.1338 275.8482 Tm +( )Tj +/C2_0 1 Tf +0.01 Tc -0.01 Tw -25.959 -1.211 Td 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second slide was used to perform the dif)17.7(ferential )]TJ +0.08 Tw -2.089 -1.211 Td +[(analysis )0.9(and )0.6(qualitative )1.9(assessments )0.6(of )0.5(the )0.9(white )0.8(and )0.7(red )0.7(blood )]TJ +0.257 Tw T* +[(cells )0.8(\(RBC\) and )0.5(platelets )1.4(in )0.5(the )0.7(CellaV)60.1(ision)]TJ +0 Tw 5.5385 0 0 5.5385 492.6196 647.0117 Tm +(\256)Tj +0.257 Tw 9.5 0 0 9.5 496.8275 643.8482 Tm +( DM96 device. )Tj +/C2_0 1 Tf +0 Tw -19.475 -1.211 Td +[<0037004B00480003>-73.3<00530055004800100046004F004400560056004C00C000460044>0.7<0057004C005200510003>-73.3<00570048005600570003>-73.2<0045005C0003>-73.5<0057004B00480003>-73.3<004800540058004C005300500048005100570003>-72.7<005A004400560003>-73.8<004800590044004F005800440057004800470003>-72.6<0045005C0003>-73.5<00440003>]TJ +/TT0 1 Tf +-0.048 Tw 0 -1.211 TD +[(pharmacists-biochemist )1.6(and in cases of disagreement )1.1(between )0.6(the )]TJ +/C2_0 1 Tf +0 Tw 0 -1.211 TD +<0055004800560058004F00570056000F00030057004B0048000300460048004F004F00560003005A00480055004800030055004800100046004F004400560056004C00C00048004700110003>Tj +/TT2 1 Tf +-0.037 Tw 10 0 0 10 331.6536 586.3482 Tm +(Statistical Analysis)Tj +/TT0 1 Tf +0.383 Tw 9.5 0 0 9.5 331.6536 563.3482 Tm +[(The sample size calculation )0.9(was performed using the )]TJ +0.097 Tw -2.089 -1.211 Td +[(program )0.6(OpenEpi, )0.6(version 2, considering )0.7(an )0.5(error of 5% and )0.5(a )]TJ +0.132 Tw 0 -1.211 TD +[(power of 80%. Statistical )1.4(analysi)0.5(s was performed )0.5(with the aid )]TJ +/C2_0 1 Tf +0 Tw 0 -1.211 TD +[<005200490003>-172.6<0057004B00480003>-172.5<0036005700440057004C00560057004C00460044004F0003>-171.3<003300440046004E0044004A00480003>-172.2<0049005200550003>-172.6<003600520046004C0044004F0003>-172<00360046004C004800510046004800560003>-172.1<000B0036003300360036000C000F0003>-173.2<0059004800550056004C005200510003>]TJ +/TT0 1 Tf +0.069 Tw T* +[(15.0, and GraphPad Prism 5. Numerical )0.9(variables )0.8(are )0.5(described )]TJ +0.121 Tw 0 -1.211 TD +[(as means and standard deviations and categorical )1(variables are )]TJ +-0.001 Tw 0 -1.211 TD +(described as proportions. Correlations were made )Tj +/Span<>> BDC +[( )249( )]TJ +EMC +20.073 0 Td +(by the Pearson )Tj +/C2_0 1 Tf +0 Tw -20.073 -1.211 Td +[<00460052005500550048004F00440057004C005200510003>-34.7<00570048005600570003>-35.3<0044005100470003>-35.7<0049005200550003>-35.6<0044004F004F0003>-35.2<004600520050005300440055>0.5<004C00560052005100560003>-35.8<00440003>-35.5<004F004800590048004F0003>-35<005200490003>-35.6<001800080003>-35.7<000B0053001000590044004F005800480003>-35.2<00940003>]TJ +T* +<0013001100130018000C0003005A0044005600030046005200510056004C0047004800550048004700030056005700440057004C00560057004C00460044004F004F005C00030056004C004A0051004C00C000460044005100570011>Tj +/TT0 1 Tf +0.703 Tw 2.089 -1.211 Td +[(The comparative )0.8(analysis between the assessments )]TJ +0.16 Tw -2.089 -1.211 Td +[(made by the CellaV)59.7(ision)]TJ +0 Tw 5.5385 0 0 5.5385 409.9988 451.5117 Tm +(\256)Tj +/C2_0 1 Tf +9.5 0 0 9.5 414.2068 448.3482 Tm +[<0003>-160.2<00270030001C00190003>-160.5<0044005100470003>-160.1<0057004B00480003>-159.8<0046004F004400560056004C00C0004600440057004C005200510003>-159.4<0045005C0003>-160.2<0057004B00480003>]TJ +/TT0 1 Tf +0.099 Tw -10.778 -1.211 Td +[(professional was made )0.5(using the )17.9(V)110.6(ariability )1.1(Statistics )18.2(T)70(able for )]TJ +/C2_0 1 Tf +0 Tw 0 -1.211 TD +[<00500044005100580044004F0003>-121.8<004F00480058004E00520046005C005700480003>-121.7<004600520058005100570056>]TJ +/TT0 1 Tf +5.5385 0 0 5.5385 408.5809 428.5117 Tm +(\(20,21\))Tj +0.123 Tw 9.5 0 0 9.5 424.7312 425.3482 Tm +[(. )18.1(The )0.9(cell )1.2(count )0.9(performed )1(by )0.5(the )]TJ +0 Tw -11.886 -1.211 Td +(professional was used as the reference in the comparisons. )Tj +/TT1 1 Tf +10.5 0 0 10.5 311.811 390.8482 Tm +(Results)Tj +/TT0 1 Tf +-0.035 Tw 9.5 0 0 9.5 331.6536 367.8482 Tm +[(The )0.8(quality )1.1(of )0.5(the )0.8(slides )0.6(produced )0.8(by )0.5(the )0.8(Sysmex)]TJ +0 Tw 5.5385 0 0 5.5385 516.8094 371.0117 Tm +(\256)Tj +-0.035 Tw 9.5 0 0 9.5 521.0174 367.8482 Tm +[( )0.5(SP-1000i )]TJ +0.117 Tw -22.022 -1.211 Td +[(device )0.5(was assessed by the CellaV)60.1(ision)]TJ +0 Tw 5.5385 0 0 5.5385 466.9248 359.5117 Tm +(\256)Tj +/C2_0 1 Tf +9.5 0 0 9.5 471.1328 356.3482 Tm +[<0003>-117.1<002700300003>-117.5<001C00190003>-117.1<004700480059004C004600480003>-116.5<00570044004E004C0051004A0003>]TJ +/TT0 1 Tf +0.249 Tw -16.771 -1.211 Td +(into account the quality of the staining, the cell localization )Tj +0.101 Tw T* +[(test )0.5(and the )0.5(percentage )0.8(of artifacts )0.9(in the )0.5(blood smear )0.5(for each )]TJ +0.208 Tw 0 -1.211 TD +[(hematocrit )1.3(level )0.8(\(T)69.9(able )0.7(2\). )54.8(All slides )0.5(evaluated )1.1(had a quality )]TJ +/C2_0 1 Tf +0 Tw T* +[<0046005200510056004C004700480055004800470003>1.1<0044005300530055005200530055004C004400570048000F0003>1.3<00490052004F004F0052005A004C0051004A0003>0.7<0057004B00480003>0.9<005700480046004B0051004C00460044004F0003>1.8<0056005300480046004C00C0004600440057004C0052005100560003>1.3<0049005200550003>]TJ +/TT0 1 Tf +0 -1.211 TD +[(quality of the CellaV)61(ision)]TJ +5.5385 0 0 5.5385 409.6592 302.0117 Tm +(\256)Tj +9.5 0 0 9.5 413.8671 298.8482 Tm +( DM96 equipment.)Tj +/C2_0 1 Tf +-8.654 -1.211 Td +[<0037004B00480003>43.3<00530055004800100046004F004400560056004C00C0004600440057004C005200510003>43.8<005200490003>42.9<0057004B00480003>43.3<004F00480058004E00520046005C005700480003>43.4<004600520058005100570003>43.3<0052004500570044004C0051004800470003>43.6<0045005C0003>43<0057004B00480003>]TJ +/TT0 1 Tf +-2.089 -1.211 Td +[(CellaV)60.1(ision)]TJ +5.5385 0 0 5.5385 356.6299 279.0117 Tm +(\256)Tj +0.063 Tw 9.5 0 0 9.5 360.8378 275.8482 Tm +( DM96 device was compared with the professional )Tj +/C2_0 1 Tf +0 Tw -5.161 -1.211 Td +[<0046004F004400560056004C00C0004600440057004C005200510003>-363.8<004400490057004800550003>-364.1<0044004A00550048004800500048005100570003>-363.7<005200550003>-364.6<0057004B00480003>-364.3<0055004800100046004F004400560056004C00C0004600440057004C005200510003>-363.6<005200490003>-364.6<0057004B00480003>]TJ +T* +[<004F00480058004E00520046005C00570048005600110003>-51.3<0037004B004C00560003>-69.3<0047004800500052005100560057005500440057004800470003>-68.6<0057004B004400570003>-69.3<0057004B00480003>-69.1<005900440055004C00440057004C005200510003>-68.7<005A004400560003>-69.6<00440046004600480053005700440045004F00480003>]TJ +0 -1.211 TD +[<004C00510003>-116<001C001300080003>-116.2<005200490003>-116.3<0057004B00480003>-115.8<0056004F004C00470048005600110003>-116.1<0032005100480003>-116.2<005200550003>-116.2<00500052005500480003>-116.1<004F00480058004E00520046005C0057004800560003>-115.6<005A0048005500480003>-116<004C00510046005200550055004800460057004F005C0003>]TJ +T* +[<0046004F004400560056004C00C0004800470003>-106.8<0049005200550003>-107.1<00520051004F005C0003>-107.1<0048004C004A004B00570003>-106.6<000B001400130008000C0003>-107.1<0056004F004C004700480056000F0003>-106.9<005A004C0057004B0003>-106.9<0057004B00480003>-106.8<00450044005100470003>-106.9<0051004800580057005500520053004B004C004F0003>]TJ +0 -1.211 TD +[<00450048004C0051004A00030057004B00480003005000520056005700030046005200500050005200510003005A005500520051004A004F005C00030046004F004400560056004C00C000480047000300460048004F004F0003000B0037>72.2<00440045004F004800030016000C00110003>]TJ +/TT0 1 Tf +0.01 Tc 0.197 Tw 2.089 -1.211 Td +[(Qualitative analysis of the RBC proved to be adequate)10.2( )]TJ +0.246 Tw -2.089 -1.211 Td +[(for the integrity of cells, uniform distribution on the slide,)10( )]TJ +0.092 Tw 0 -1.211 TD +[(morphology and staining for all of the slides produced by the)10.1( )]TJ +-0.01 Tw T* +(Sysmex)Tj +0 Tc 0 Tw 5.5385 0 0 5.5385 342.4675 175.5117 Tm +(\256)Tj +0.01 Tc 0.312 Tw 9.5 0 0 9.5 346.7308 172.3482 Tm +[( SP-1000i device. )17.2(The RBC analysis provided by)10( )]TJ +-0.01 Tw -3.676 -1.211 Td +[(CellaV)59.4(ision)]TJ +0 Tc 0 Tw 5.5385 0 0 5.5385 357.6749 164.0117 Tm +(\256)Tj +0.01 Tc 0.089 Tw 9.5 0 0 9.5 361.9382 160.8482 Tm +[( DM96 was not used for comparison, leaving the)10.1( )]TJ +-0.021 Tw -5.277 -1.211 Td +[(qualitative evaluation of the RBC as well as qualitative changes)9.8( )]TJ +/C2_0 1 Tf +-0.01 Tw 0 -1.211 TD 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