| A 24-year-old male with no comorbidities. | |
| Andrological history: testicular decrease at birth, not circumcised, appearance of normal secondary sexual characters at 14 years, spermarche at 14 years, onset of sexual life at 16 years, two sexual partners. | |
| Make sure libido is preserved without ejaculatory problems 4/7. | |
| Physical location: Male fetus without characteristic fascies, without cardiopulmonary alterations, normal breasts according to age and sex. | |
| Distribution of the android hair, penis with retractile foreskin, central meatus, left testicle with decreased volume compared to the contralateral testicle, with decreased consistency and normal varicocele varicocele grade III; | |
| Current situation: infertility of 1 year and a half evolution, female partner of 23 years, with fertility study protocol without alterations. | |
| Laboratory: Blood biometry: hemoglobin 15.3 g/dl, leukocytes 8,000 cells/μ, TP 13.8 seconds, TTP 28.9 seconds. | |
| Blood chemistry: glucose 80 mg/dl, creatinine 0.96 mg/dl, sodium 142 meq/L, potassium 4.4 meq/L, chlorine 103 meq/L. | |
| Urine density: pH 6, 1.020, leukocytes 2-4 per field. | |
| Horizontal: LH 5.2 (7.6 mIU/ml) FSH 12.2 (0.7-11.1 mIU/ml), oestradiol 12.470 (0-56 pg/ml) testosterone 538 ng/-17). | |
| Negative urine and semen cultures: Chlamydia, Mycoplasma and Ureaplasma search in negative urethral secretion. | |
| Semen analysis: Normal aspect, normal liquefaction, normal viscosity, volume 3 ml, pH 8.0, concentration 3 million/ml, motility type d=100%. | |
| Morphology (Kaiser): normal 0%, abnormal tail 28%, amorphous 36%, beaked 28%, pinch 7%, duplicate 1%. | |
| Malignant spermia, congenital spermia, and severe teratozoospermia. | |
| Ultrasound examination: Left testicle 4.68 cc with varicocele, right testicle 10.92 cc, both epididymis normal. | |
| Karyotype: 46,XY. | |
| Y chromosome microdeletions by multiplex polymerase chain reaction of Y chromosome STS: | |
| Multipleplex A: | |
| ZFY, SRY, sY84 (AZFa) present. | |
| sY254 (AZFc), sY127 (AZFb) absent. | |
| Multiple B: | |
| ZFY, SRY, sY86(AZFa) present. | |
| sY134 (AZFb), sY255 (AZFc) absent. | |
| The analyses were performed in triplicate and as reference were taken positive and negative controls. | |