diff --git a/PL9fwy3NUQKwY9jl8xw-bWkVZkWcGGyspd/-PkMjabV1O4.srt b/PL9fwy3NUQKwY9jl8xw-bWkVZkWcGGyspd/-PkMjabV1O4.srt
new file mode 100644
index 0000000000000000000000000000000000000000..f5291ac2e9f478f371930cc74f505e79837da8ce
--- /dev/null
+++ b/PL9fwy3NUQKwY9jl8xw-bWkVZkWcGGyspd/-PkMjabV1O4.srt
@@ -0,0 +1,2271 @@
+1
+00:00:03,490 --> 00:00:07,770
+مرحبا صبايا، هلأ احنا لما شرحنا محاضرة solution
+
+2
+00:00:07,770 --> 00:00:11,530
+used in medical laboratory، كان فيه جزئية طبعا أنّه
+
+3
+00:00:11,530 --> 00:00:18,210
+هي بدها تفاعل وبدها شرح يعني على سبورة أو على ورق
+
+4
+00:00:18,210 --> 00:00:23,330
+لحتى توضح الفكرة، فأنا حكيت لكم أنّه راح أصور لكم
+
+5
+00:00:23,330 --> 00:00:28,130
+محاضرة أخرى كملحق لها، علشان أنّه تدرسوها مع بعض و
+
+6
+00:00:28,130 --> 00:00:30,410
+توضح الفكرة، خصوصا في جزئية dilution
+
+7
+00:00:41,500 --> 00:00:47,020
+طبعا أنا بدأت بالـ... بالجزئية الأولى للمحاضرة، يعني
+
+8
+00:00:47,020 --> 00:00:52,200
+حكيت عن الـ... عن المحاليل وأنواعها، وشرحت بعض
+
+9
+00:00:52,200 --> 00:00:56,610
+الأفكار عنها، وبعدين حكينا عن وحدات القياس، ومن
+
+10
+00:00:56,610 --> 00:01:01,270
+ثمّ الـ dilution، فبرضه هذه المحاضرة شاملة، ويعني
+
+11
+00:01:01,270 --> 00:01:06,250
+المهم أنّه تدرسوها جنب تسجيل المحاضرة الأساسي اللي
+
+12
+00:01:06,250 --> 00:01:09,810
+نزل على الـ model، طبعا هلأ أنا راح أبلش أشغل
+
+13
+00:01:09,810 --> 00:01:14,390
+الفيديو، هيبدأ يعرض لكم الفيديو اللي مصور أصلا سابقا
+
+14
+00:01:14,390 --> 00:01:18,750
+مسبقا وبيشرح المحاضرة، السلام عليكم ورحمة الله
+
+15
+00:01:18,750 --> 00:01:23,920
+وبركاته، طالباتي الكريبات مستوى... الكريبات مستوى الثاني
+
+16
+00:01:23,920 --> 00:01:28,200
+قسم معلم طبية مقبلة، أهلا وسهلا بكم إن شاء الله
+
+17
+00:01:28,200 --> 00:01:34,480
+اللي رح اليوم نعطي تكملة المحاضرة اللي حكينا رح
+
+18
+00:01:34,480 --> 00:01:38,780
+نشرحها على ورق، مُقَدَّمَة بأمثلة، لحتى يُعمَق الفهم
+
+19
+00:01:38,780 --> 00:01:42,890
+للمحاضرة المسجلة، كانت المحاضرة بتحكي عن solution
+
+20
+00:01:42,890 --> 00:01:46,450
+used in medical laboratories، وحكينا في المحاضرة
+
+21
+00:01:46,450 --> 00:01:49,210
+أنّها فيها من الـ... فيها أنواع من المحاليل
+
+22
+00:01:49,210 --> 00:01:53,170
+المستخدمة في المختبرات الطبية، من هذه المحاليل
+
+23
+00:01:53,170 --> 00:01:58,700
+الرئيسية أربعة أنواع: الصبغات، الـ buffer، و الـ standard
+
+24
+00:01:58,700 --> 00:02:02,860
+حكينا عن الـ reagent، هو اللي بيعمل الـ change، طبعا
+
+25
+00:02:02,860 --> 00:02:06,180
+هو المادة المتفاعلة اللي بتضيفها على العينة، بتعمل
+
+26
+00:02:06,180 --> 00:02:12,680
+change، تغيير measurable or noticeable، يعني إمّا يكون
+
+27
+00:02:12,680 --> 00:02:17,840
+يمكن قياسه أو يمكن ملاحظته، هذا التغير اللي يحدثه
+
+28
+00:02:17,840 --> 00:02:21,740
+على العينة، بنجرده، أنه يُفتح على العينة للكشف عن
+
+29
+00:02:21,740 --> 00:02:27,310
+مادة معينة أو يعني... يعني عن جزء من العينة مراقبة أو
+
+30
+00:02:27,310 --> 00:02:31,730
+مراد فحصه، النوع الآخر من المحاليل كان هو الصبغات
+
+31
+00:02:31,730 --> 00:02:35,230
+النوع الثاني من المحاليل حكيناه اللي هو الصبغات
+
+32
+00:02:35,230 --> 00:02:40,310
+اللي بيعطيني مكون من، طبعا، من colored dyes، اللي هي
+
+33
+00:02:40,310 --> 00:02:44,570
+صبغات ملونة، تكسب العينة artificial color، أو لون
+
+34
+00:02:44,570 --> 00:02:48,090
+مكتسب أو لون اصطناعي، لحتى يتمكن من رؤيتها تحت الـ
+
+35
+00:02:48,090 --> 00:02:51,780
+microscope، النوع الثالث من المحاليل كان الـ buffer، اللي
+
+36
+00:02:51,780 --> 00:02:55,660
+هو المحلول  الذي يُوازن البيئة، هذا النوع من المحاليل
+
+37
+00:02:55,660 --> 00:02:59,800
+يُحافظ على بيئة، هذا وظيفته، ومن خصائصه أنّه يُقاوم resist
+
+38
+00:02:59,800 --> 00:03:04,400
+change، التغير في البيئة، النوع الرابع
+
+39
+00:03:04,400 --> 00:03:07,640
+اللي هو الـ standard، طبعا رح أحكيه مش بالترتيب زي ما
+
+40
+00:03:07,640 --> 00:03:12,700
+هوّ بالمحاضرة، يعني لأ، بس لذكر الـ standards، هو
+
+41
+00:03:12,700 --> 00:03:16,840
+المحاليل القياسية، طبعا الـ standard هي عبارة عن
+
+42
+00:03:16,840 --> 00:03:20,940
+محاليل قياسية أو معيارية، وأهم ما يميزها أنّها non
+
+43
+00:03:20,940 --> 00:03:25,880
+-concentration، طبعا non-concentration، تركيز معلوم
+
+44
+00:03:25,880 --> 00:03:31,040
+طبعا احنا حكينا أنّه استخدام الـ standard solution
+
+45
+00:03:31,040 --> 00:03:36,120
+أنّه لأن تركيزها بيكون معلوم، بجيب تركيز المجهول
+
+46
+00:03:36,120 --> 00:03:41,640
+في العينة بدلالته، هذا المعلوم، كيف يحدث هذا؟ لو فرضت
+
+47
+00:03:41,640 --> 00:03:45,580
+أنّه أنا في عندي عينة فيها sample، في... طبعا، النبأ
+
+48
+00:03:45,580 --> 00:03:50,820
+فيها عينة، والنبأ الآخر فيه محلول standard، طبعا اللي
+
+49
+00:03:50,820 --> 00:03:53,740
+لو أنا بدي أقيس مستوى السكر في الدم، بدي أقيس الـ
+
+50
+00:03:53,740 --> 00:03:57,700
+glucose اللي موجود في الدم، وهذا المحلول طبعا أكيد
+
+51
+00:03:57,700 --> 00:04:00,560
+هيكون محلول glucose معلوم التركيز، عشان أنا أقرأ
+
+52
+00:04:00,560 --> 00:04:04,340
+نوع العينة، ما بنفعش مثلا أنا، بدي أقيس السكر
+
+53
+00:04:04,340 --> 00:04:07,660
+أجيب الـ standard محلول بروتين، لأ، لازم يكون من
+
+54
+00:04:07,660 --> 00:04:12,220
+نفس النوع، علشان أنا أستخدمه كـ... كمحلول معلوم
+
+55
+00:04:12,220 --> 00:04:17,300
+التركيز، وأجيب المجهول بدلالته، طبعا هذا بالنسبة للـ
+
+56
+00:04:17,300 --> 00:04:22,700
+لـ sample، بدي أ... أ... أشوف الـ... بدي أقيس
+
+57
+00:04:22,700 --> 00:04:26,160
+تركيز من الـ glucose اللي موجود فيها، والـ standard هي
+
+58
+00:04:26,160 --> 00:04:29,200
+محلول معياري، موجود فيه glucose، محلول اللي
+
+59
+00:04:29,200 --> 00:04:32,920
+هو معلوم التركيز من الـ glucose، بدي أنا من خلال الـ
+
+60
+00:04:32,920 --> 00:04:40,410
+...التجربة، أستخدمه كمحلول معياري أو قياسي، أول شيء
+
+61
+00:04:40,410 --> 00:04:43,410
+بدي أعمله، بدي أضيف الـ reagent، هو اللي هي المادة
+
+62
+00:04:43,410 --> 00:04:47,490
+المتفاعلة، على كل من الـ sample و الـ standard، راح
+
+63
+00:04:47,490 --> 00:04:50,850
+أضيف الـ reagent، المادة المتفاعلة، طبعا المادة
+
+64
+00:04:50,850 --> 00:04:54,050
+المتفاعلة، هي اللي... اللي... اللي راح تعطيني change،
+
+65
+00:04:54,050 --> 00:04:58,070
+راح تعطيني تغيير، organic metabolic change، طبعا
+
+66
+00:04:58,070 --> 00:05:02,250
+هتتفاعل مع الـ glucose، وتعطني تغيير، تغيير ممكن يكون
+
+67
+00:05:02,250 --> 00:05:06,430
+في اللون أو كذا، احنا ضربنا مثال، أنّه إذا أنا فرضت
+
+68
+00:05:06,430 --> 00:05:11,670
+أنّه في الـ... في الـ... فرد تغير اللون، ويعطي لي لون
+
+69
+00:05:11,670 --> 00:05:17,030
+pink، مثلا، لو مثلا، هو أعطاني لون pink، تمام، و هو
+
+70
+00:05:17,030 --> 00:05:23,410
+أعطاني أيضا لون pink آخر، على فرض أنّه التركيز هنا
+
+71
+00:05:23,410 --> 00:05:27,330
+عبارة عن 100 milligram بالديسيلتر، بالنسبة للـ
+
+72
+00:05:27,330 --> 00:05:32,750
+standard، تمام؟ 100 milligram بالديسيلتر، وأنا دخلت
+
+73
+00:05:32,750 --> 00:05:35,590
+العينة اللي متستخدم بالها، دي، والـ standard عادي
+
+74
+00:05:35,590 --> 00:05:39,590
+هذه تقيس شدة اللون، على فرض أنّه أعطاني شدة اللون
+
+75
+00:05:39,590 --> 00:05:44,090
+كانت اثنين، وأنا أعطاني واحد، طبعا إذا هنا شدة اللون
+
+76
+00:05:44,090 --> 00:05:48,730
+نصف شدة اللون للـ standard، معناه ايه؟ التركيز إذا
+
+77
+00:05:48,730 --> 00:05:52,470
+كان هنا مئة، معناه يكون التركيز هنا خمسين، على فرض
+
+78
+00:05:52,470 --> 00:05:58,890
+أنّه أعطاني... على فرض أنّه أعطاني شدة لون أقوى هنا
+
+79
+00:05:58,890 --> 00:06:04,660
+بيني، طبعا ف... والجهاز قاسها على أساس أنها هي
+
+80
+00:06:04,660 --> 00:06:10,680
+أربعة، طبعا وهنا اثنين، فلنفرض أنّه الاثنين كانت
+
+81
+00:06:10,680 --> 00:06:13,540
+تركيزها مئة، طبعا الاثنين كانت تركيزها مئة
+
+82
+00:06:13,540 --> 00:06:16,100
+الأربعة كانت راح تكون تركيزها... راح تكون
+
+83
+00:06:16,100 --> 00:06:19,740
+تركيزها مئتين، اللي هو ضعفها، فبالتالي بهذا الشكل
+
+84
+00:06:19,740 --> 00:06:24,640
+أو بهذا المبدأ، أنا بجيب تركيز المواد أو المكونات
+
+85
+00:06:24,640 --> 00:06:29,700
+للعينة، طبعا المجهولة، بدلالته، معلومة، لو هو الـ
+
+86
+00:06:29,700 --> 00:06:32,900
+standard، هذا... هذا فكرة استخدام الـ standard
+
+87
+00:06:32,900 --> 00:06:36,480
+solution في معرفة التركيز... التركيز المجهول،
+
+88
+00:06:36,480 --> 00:06:40,480
+طبعا، و هذا دور الـ agent، دور الـ agent هو يكشف، أنه
+
+89
+00:06:40,480 --> 00:06:44,900
+يعطيه change، يعطيه تغيير، طبعا؟ الـ... الـ... الـ...
+
+90
+00:06:44,900 --> 00:06:48,260
+إذا هيك خلصنا، وشرحنا الـ reagent والـ standard
+
+91
+00:06:48,260 --> 00:06:51,600
+أكثر، الـ stain حكينا أنّه هو عبارة عن صبغة، غلطت
+
+92
+00:06:51,600 --> 00:06:56,020
+مكونة من colored dye، وبت... تكسب الـ... الـ... مكونات
+
+93
+00:06:56,020 --> 00:06:59,720
+العينة artificial color، لحتى نتمكن من رؤيتها تحت
+
+94
+00:06:59,720 --> 00:07:03,580
+الـ microscope، من خلال هذه الصبغات، أنا بتمكن رؤية
+
+95
+00:07:03,580 --> 00:07:08,720
+العينات بشكل أوضح، ومكوناتها سواء كانت خلايا، أملاح،
+
+96
+00:07:08,720 --> 00:07:15,070
+ميكروبات، بكتيريا، فطريات، فيروسات، كل أنواع المكونات
+
+97
+00:07:15,070 --> 00:07:18,150
+الداخلية للخلية، ممكن صباغها ورؤيتها تحت الـ
+
+98
+00:07:18,150 --> 00:07:22,250
+microscope، بالنسبة للـ buffer هو عبارة عن محلول، حكينا
+
+99
+00:07:22,250 --> 00:07:26,390
+بقي، بيتكون، بيقوم بتنظيم الـ pH، وحكينا أنّه
+
+100
+00:07:26,390 --> 00:07:32,710
+بدّه يكون مكوّن من شيء، إما من weak acid مع الـ strong
+
+101
+00:07:32,710 --> 00:07:39,350
+base تبعته، أو الـ strong acid و الـ weak base تبعته، و
+
+102
+00:07:39,350 --> 00:07:43,810
+base،  في المحاضرة شرحت لكم أنّه ممكن يكون مُقَوَّم من الـ
+
+103
+00:07:43,810 --> 00:07:47,350
+weak acid و الـ weak salt تبعته، هي نفس المعنى،
+
+104
+00:07:47,350 --> 00:07:51,710
+خلينا نضرب مثال اللي هي عندنا CH3COOH، اللي
+
+105
+00:07:51,710 --> 00:07:56,790
+هو عبارة عن هذا acetic acid، تمام؟ acetic acid، الـ
+
+106
+00:07:56,790 --> 00:08:01,510
+acetic acid هذا عبارة عن weak acid، تمام؟ ممكن أنا
+
+107
+00:08:01,510 --> 00:08:06,830
+أكون ب استخدمه مع وجود الـ CH3COO−، اللي هو
+
+108
+00:08:06,830 --> 00:08:10,330
+الـ أيون المترافق تبعه، الـ أيون المترافق تبعه، هذا
+
+109
+00:08:10,330 --> 00:08:14,950
+بسمّيه acetate، تمام، وهو عبارة عن ملح، وهو اللي
+
+110
+00:08:14,950 --> 00:08:20,660
+بيخليه أنّه strong base، طبعا هو متأين، بيكون له خاصية
+
+111
+00:08:20,660 --> 00:08:24,840
+strong base، هذول مع بعض بيكونوا متوازنين، إذا إمّا
+
+112
+00:08:24,840 --> 00:08:28,060
+weak acid و strong base، أو weak acid والـ... والـ
+
+113
+00:08:28,060 --> 00:08:31,760
+...والـ conjugate base، أو weak acid و الـ salt تبعه،
+
+114
+00:08:31,760 --> 00:08:36,340
+نفس المعنى، طبعا هذا بالنسبة للـ buffer، والمكونات، طبعا
+
+115
+00:08:36,340 --> 00:08:41,680
+حكينا، بالنسبة لمحاليل، هذه أيضا
+
+116
+00:08:41,680 --> 00:08:44,880
+الاعتبارات اللي بتتطرق، بتُطرح أثناء استخدامها أو
+
+117
+00:08:44,880 --> 00:08:49,420
+أثناء تحضيرها، ويبقى معلومات عن كيفية الحفظ، والخواص
+
+118
+00:08:49,420 --> 00:08:53,780
+اللي لازم تتوفر فيها، علشان أنا أعتبرهم سواء كانت
+
+119
+00:08:53,780 --> 00:08:56,960
+reagent أو أعتبرهم standard، أو أعتبرهم buffer، وهكذا
+
+120
+00:08:57,430 --> 00:09:01,410
+طيب بعد هيك حكينا... طبعا حكينا عن أنّه الـ
+
+121
+00:09:01,410 --> 00:09:04,330
+standard solution، أنّه primary standard و secondary
+
+122
+00:09:04,330 --> 00:09:09,210
+standard، وفي النهاية حكينا أنّه المحاليل هذه لما
+
+123
+00:09:09,210 --> 00:09:14,830
+أنا بدي أعبر عن التركيز تبعها، لازم أنا أستخدم
+
+124
+00:09:14,830 --> 00:09:20,930
+التركيز، أحكي الـ concentration و الـ unit تبعته، طبعا
+
+125
+00:09:20,930 --> 00:09:25,130
+أحكي عن الـ concentration و الـ unit، ما بنفعش مثلا أحكي
+
+126
+00:09:25,130 --> 00:09:28,510
+تركيز المحلول، ضربنا مثلا في المحاضرة المسجلة، أقول
+
+127
+00:09:28,510 --> 00:09:32,250
+إنّي أنا ما بنفعش أنا أجيب محلول تركيزه خمسين، وأقول
+
+128
+00:09:32,250 --> 00:09:37,330
+إنّه خمسين، بس، طب خمسين أيش؟ طب يعني... طب يعني
+
+129
+00:09:37,330 --> 00:09:42,650
+أنا بيفهم هو... هو يعني بأي وحدة، علشان أعرف مدى
+
+130
+00:09:42,650 --> 00:09:47,310
+قوة التركيز تبعه، فبالتالي أنّه لازم أنا أعبر عن الـ
+
+131
+00:09:47,310 --> 00:09:51,350
+...الـ... المحلول بـ... الـ concentration بالرقم و الـ
+
+132
+00:09:51,350 --> 00:09:55,690
+unit، طبعا وحكينا أنّه في عندي نوعين من الـ units، من
+
+133
+00:09:55,690 --> 00:10:01,070
+الوحدات بتستخدم للتعبير عن الـ concentration، في نوع
+
+134
+00:10:01,070 --> 00:10:08,950
+اسمه physical، طبعا physical units، ونوع آخر بنسميه
+
+135
+00:10:08,950 --> 00:10:15,580
+اللي هو الـ chemical units، طبعا؟ طيب، ماشي، بنحكيها
+
+136
+00:10:15,580 --> 00:10:18,340
+على الـ physical، يعني نسميها اسمها وحدات فيزيائية، يعني
+
+137
+00:10:18,340 --> 00:10:25,800
+بنعبر بالوزن مثلا، بالغرام، الطول مثلا الـ length
+
+138
+00:10:25,800 --> 00:10:26,580
+بالـ centimeter
+
+139
+00:10:29,290 --> 00:10:33,810
+الحجم بالـ ml، هذه الوحدات الفيزيائية اللي أنتم
+
+140
+00:10:33,810 --> 00:10:38,050
+أخذتوها أكيد، يعني أعتقد من طول مرات معاكم في مساقات
+
+141
+00:10:38,050 --> 00:10:43,410
+الفيزياء، فطبعا لو في عندي ثلاثة أنواع من الوحدات
+
+142
+00:10:43,410 --> 00:10:50,110
+اللي هم weight to volume، و weight to weight، أو
+
+143
+00:10:50,110 --> 00:10:55,150
+weight per volume، weight per weight، و volume per
+
+144
+00:10:55,150 --> 00:10:59,030
+volume، أنا بحكيها weight to volume ratio، نفس
+
+145
+00:10:59,030 --> 00:11:01,390
+الكلام، يعني لو هو weight to volume أو weight per
+
+146
+00:11:01,390 --> 00:11:05,070
+volume، نفس المعنى، طيب، مفروض أن فيه محلول مثلا
+
+147
+00:11:05,070 --> 00:11:10,340
+هذا المحلول مثلا NaCl، طبعا محلول ملح الطعام، الـ NaCl
+
+148
+00:11:10,340 --> 00:11:14,580
+هذا تركيزه ثلاثين في المئة، طبعا خلينا نشوف إيش معنى
+
+149
+00:11:14,580 --> 00:11:18,040
+كلمة ثلاثين في المئة، طبعا هو موجود في وسط المذيب،
+
+150
+00:11:18,040 --> 00:11:24,680
+الـ solvent، طبعا، طيب محلول NaCl، الـ solute عندي NaCl
+
+151
+00:11:24,680 --> 00:11:28,800
+طبعا، والـ solvent، الـ distilled water، احنا حكينا
+
+152
+00:11:28,800 --> 00:11:32,360
+أنّه أي محلول بيكون مكون من solute، تمام، اللي هي
+
+153
+00:11:32,360 --> 00:11:36,800
+المادة المذابة، والـ solvent، المذيب، تمام، الـ NaCl هي
+
+154
+00:11:36,800 --> 00:11:40,500
+المادة المذابة، والـ solvent، الـ distilled water هو
+
+155
+00:11:40,500 --> 00:11:44,340
+المذيب، خلينا نحكي أنّه تركيزه ثلاثين بالمئة، خلينا
+
+156
+00:11:44,340 --> 00:11:49,060
+نشوف إيش يعني ثلاثين بالمئة، لو ثلاثين بالمئة، لو كانت
+
+157
+00:11:49,060 --> 00:11:55,700
+الوحدة weight per volume، لو كانت الوحدة ثلاثين بالمئة weight
+
+158
+00:11:55,700 --> 00:12:02,380
+per volume، طبعا من الـ... من الـ NaCl، معناها أنا
+
+159
+00:12:02,380 --> 00:12:08,500
+استخدمت ثلاثين غرام، طبعا هذا... هذا solute، وهذا
+
+160
+00:12:08,500 --> 00:12:14,360
+solvent، بيكون، طبعا أنا استخدمت ثلاثين غرام من الـ
+
+161
+00:12:14,360 --> 00:12:20,300
+solute، اللي هو NaCl، ثلاثين غرام
+
+162
+00:12:20,300 --> 00:12:29,230
+طبعا من NaCl، وأخذت كمان مئة ml
+
+163
+00:12:29,230 --> 00:12:35,410
+ml من وين؟ من المذيب، اللي هو عبارة عن distilled water، هذا كان في حال لو أنا استخدمت ثلاثين
+
+164
+00:12:35,410 --> 00:12:40,320
+كمية weight per volume، NaCl، طيب لو كانت عندي ثلاثين
+
+165
+00:12:40,320 --> 00:12:46,240
+كمية weight per volume، أو sorry، weight per weight
+
+166
+00:12:46,240 --> 00:12:51,080
+طبعا NaCl، إيش نعمل؟ معناها طبعا احنا عارفين أن هذا
+
+167
+00:12:51,080 --> 00:12:57,400
+للـ solute، وهذا للـ solvent، طبعا ثلاثين غرام، اللي هي
+
+168
+00:12:57,400 --> 00:13:04,350
+غرام، على سبعين غرام، هما ب... هما بحطوا مية كاملة زي
+
+169
+00:13:04,
+
+223
+00:17:21,170 --> 00:17:24,850
+وزن تبعين وتسعين على اتنين ويساوي تسعة وأربعين
+
+224
+00:17:26,410 --> 00:17:31,710
+طبعاً إيش الوحدات هنا؟ بتطلع هنا بالـ normal أنتَ
+
+225
+00:17:31,710 --> 00:17:35,170
+بتنسى و  قدرها normal هنا الـ .. الـ .. الـ molarity
+
+226
+00:17:35,170 --> 00:17:39,650
+لما أنا أحسبها بتطلع عندي العرض ووحدة القياس
+
+227
+00:17:39,650 --> 00:17:44,430
+molar طبعاً لأنها molarity هذه هي الـ chemical units
+
+228
+00:17:44,430 --> 00:17:51,310
+إذا حكينا عن المحاليل اللي بتستخدم في الـ .. في
+
+229
+00:17:51,310 --> 00:17:57,190
+المختبرات الطبية وحكينا كيف أنا بأقارن الـ .. بأجيب
+
+230
+00:17:57,190 --> 00:18:00,170
+التركيز الموجود في العينة بدلالة الـ standard إيش
+
+231
+00:18:00,170 --> 00:18:05,410
+الفكرة بالظبط وحكينا أيضاً عن الوحدات اللي هي الـ
+
+232
+00:18:05,410 --> 00:18:09,470
+physical units والـ chemical units والتطبيق عليها
+
+233
+00:18:09,470 --> 00:18:14,090
+تمام؟ بعد هيك انتقلنا لحتى نحكي عن الـ dilution
+
+234
+00:18:14,090 --> 00:18:19,410
+تمام؟ في المحاضرة المسجلة الـ dilution التخفيف
+
+235
+00:18:19,980 --> 00:18:24,480
+أعطيتكم مثال، حكيت لكم أنه لو أنتِ عندك كوب حطيتِ
+
+236
+00:18:24,480 --> 00:18:30,140
+فيه معلقة سكر، طبعاً خلينا نفترض أنك أنتِ ضفتِ معلقة
+
+237
+00:18:30,140 --> 00:18:35,300
+سكر هنا وحطيتِها و
+
+238
+00:18:35,300 --> 00:18:47,600
+عبيتي لنصها ماء، طبعاً فهنا سكر sugar حطيه هنا طيب
+
+239
+00:18:47,600 --> 00:18:51,620
+إحنا حاطين نفس المعلقة، نفس الكمية وأنا عبيتها
+
+240
+00:18:51,620 --> 00:18:59,020
+لآخرها ماء، طبعاً إذا .. إذا الـ solute دي اسمها عبارة
+
+241
+00:18:59,020 --> 00:19:05,380
+عن sugar والـ solvent من distilled water بس أنا
+
+242
+00:19:05,380 --> 00:19:11,840
+عبيّت لنصّ، وأنا عبيّت للكاسة كاملة، فاليومين رح يكون
+
+243
+00:19:13,740 --> 00:19:18,460
+طبعاً أكيد هذا طبعاً هيكون اللي هيدي sweeter
+
+244
+00:19:18,460 --> 00:19:23,340
+sweet أو اللي هيدي more sweet، هذا لسة sweet هيكون
+
+245
+00:19:23,340 --> 00:19:24,060
+صح؟
+
+246
+00:19:26,640 --> 00:19:32,800
+ليش؟ لأن أنا ضفت من الـ solvent أكثر، خففت أكثر
+
+247
+00:19:32,800 --> 00:19:40,740
+طيب، أصبح التخفيف هو عبارة عن أني أقلل من الـ
+
+248
+00:19:40,740 --> 00:19:45,040
+concentration من خلال أني إيش أعمل؟ أضيف
+
+249
+00:19:45,040 --> 00:19:49,620
+solvent وهذا هو التعريف اللي موجود في الفيديو هو
+
+250
+00:19:49,620 --> 00:19:55,800
+أنّي أعمل decrease للـ concentration من خلال أن
+
+251
+00:19:55,800 --> 00:20:01,140
+أنا addition أعمل addition لزيادة المزيد من الـ
+
+252
+00:20:01,140 --> 00:20:05,320
+solvent هذا تعريف الـ dilution طبعاً وحكينا أن الـ
+
+253
+00:20:05,320 --> 00:20:09,620
+dilution يُستخدم كثيراً في المختبرات الطبية في تحديد
+
+254
+00:20:09,620 --> 00:20:15,390
+المحاليل فلازم إحنا نستوعب فكرة أنّه حكينا أن أنا في
+
+255
+00:20:15,390 --> 00:20:20,790
+عندي نوعين من الـ dilution، نوع بنسميه simple
+
+256
+00:20:20,790 --> 00:20:26,390
+dilution، dilution بسيط ونوع آخر بنسميه الـ
+
+257
+00:20:26,390 --> 00:20:32,510
+dilution الـ serial، الـ serial dilution إيش يعني
+
+258
+00:20:32,510 --> 00:20:38,810
+simple dilution؟ يعني بخطوة واحدة، بيصير عدة
+
+259
+00:20:38,810 --> 00:20:40,370
+multiple
+
+260
+00:20:44,230 --> 00:20:48,650
+steps وطبعاً موجودة كلمة multiplier بالشرائح دي
+
+261
+00:20:48,650 --> 00:20:56,390
+multiplicator progressive steps طبعاً هذه عدة خطوات
+
+262
+00:20:56,390 --> 00:20:59,410
+مع
+
+263
+00:20:59,410 --> 00:21:03,230
+السلامة يا عزيزي يا عزيزي يا عزيزي، عدد خطوات طبعاً
+
+264
+00:21:03,230 --> 00:21:08,030
+هذا الفرق ما بين الاثنين، بالشرائح يعني طيب إيش يعني
+
+265
+00:21:08,030 --> 00:21:11,850
+يعني مثلاً لما أنا يكون في عندي مادة بسميها خلينا
+
+266
+00:21:11,850 --> 00:21:15,510
+نسميها stock أو اسميها original المادة الأصلية
+
+267
+00:21:15,510 --> 00:21:20,510
+اللي أنا بدي أخففها، لو أنا أخذت منها volume طبعاً
+
+268
+00:21:20,510 --> 00:21:26,170
+وحطيته في الـ tube تبعتي طبعاً، واضفت هنا solvent
+
+269
+00:21:26,170 --> 00:21:32,990
+ما بعرف أنا بعد ما أبقى عافية، عدة خطوات طبعاً هذا
+
+270
+00:21:32,990 --> 00:21:38,470
+الفرق ما بينها بالشرائح، طيب إيش يعني؟ يعني مثلاً
+
+271
+00:21:38,470 --> 00:21:41,430
+لما أنا يكون في عندي مادة بسميها خلينا نسميها
+
+272
+00:21:41,430 --> 00:21:44,970
+stock أو بسميها original المادة الأصلية اللي أنا
+
+273
+00:21:44,970 --> 00:21:50,710
+بدي أخففها، لو أنا أخذت منها volume طبعاً وحطيته في
+
+274
+00:21:50,710 --> 00:21:58,420
+الـ tube تبعتي طبعاً، واضفت هنا solvent معناها أنا هنا
+
+275
+00:21:58,420 --> 00:22:01,760
+بخطوة واحدة، يعني أني أنا بصّ .. نقلت منها لتيوب ونقلت
+
+276
+00:22:01,760 --> 00:22:06,200
+نقلت solvent هذا بسميه simple لكن الـ serial أني أنا
+
+277
+00:22:06,200 --> 00:22:12,500
+هيكون .. يكون عندي stock تمام؟ وأنقل من خطّ .. منه
+
+278
+00:22:12,500 --> 00:22:16,680
+لتيوب ومنه أيضاً لتيوب أخرى وهكذا لتيوب أخرى
+
+279
+00:22:16,680 --> 00:22:20,940
+فيكون متسلسل، يعني معقد أكثر فهذا بسميه serial
+
+280
+00:22:22,130 --> 00:22:28,070
+حكينا معلومة أنّه الـ serial بيبدأ كـ simple يعني أول
+
+281
+00:22:28,070 --> 00:22:32,950
+خطوة في الـ serial هي عبارة عن simple طبعاً أول خطوة
+
+282
+00:22:32,950 --> 00:22:39,330
+في الـ serial عبارة عن simple بعدين بكمل كل ما
+
+283
+00:22:39,330 --> 00:22:43,130
+اتعقد أكثر بقلّ الـ simple الـ serial اسمه بيصير
+
+284
+00:22:43,130 --> 00:22:46,890
+الـ serial وهكذا، هاي الفرق ما بين simple dilution
+
+285
+00:22:46,890 --> 00:22:50,450
+وsimple serial dilution، هلّأ بناخذ أمثلة على كل من
+
+286
+00:22:50,450 --> 00:22:54,550
+الـ simple dilution والـ serial dilution لحتى نعرف
+
+287
+00:22:54,550 --> 00:22:59,330
+كيف إحنا بنحل المسائل المتعلقة بهذا الـ .. الـ ..
+
+288
+00:22:59,330 --> 00:23:04,570
+الشيء ده، خلينا نحكي عن أول شيء الـ simple dilution
+
+289
+00:23:04,570 --> 00:23:10,010
+simple
+
+290
+00:23:10,010 --> 00:23:10,570
+dilution
+
+291
+00:23:13,350 --> 00:23:18,150
+حكينا أنه يحدث أو يجري بخطوة واحدة، يعني مثلاً
+
+292
+00:23:18,150 --> 00:23:22,110
+نفترض أني في عندي تيوب تركيزها مثلاً مئتين
+
+293
+00:23:22,110 --> 00:23:29,990
+ملي جرام بالأقل، أو بالغرام تمام؟ فيها محلول يوليّة تمام؟
+
+294
+00:23:29,990 --> 00:23:34,710
+مهدّئ اسمه يوليّة، لو أنا بدي آخذ منها one
+
+295
+00:23:34,710 --> 00:23:39,750
+مل و أحطها في تيوب، والتيوب بتاعتها تسعة مل من
+
+296
+00:23:39,750 --> 00:23:42,790
+الـ solvent اللي بنفترض أو نعتقد أنه distilled
+
+297
+00:23:42,790 --> 00:23:49,630
+water، إذا واحد مل من المحلول اللي تركيزه مئتين
+
+298
+00:23:50,040 --> 00:23:54,240
+طبعاً هذا الواحد الملّي اللي كان تركيزه 200 أنا
+
+299
+00:23:54,240 --> 00:23:58,240
+دي حطيته وضفت عليه 9 أضعاف الكمية من الـ distill
+
+300
+00:23:58,240 --> 00:24:02,040
+water، يعني حطيت واحد مل وحطيت عليه 9 كمان من
+
+301
+00:24:02,040 --> 00:24:05,800
+الـ distill water، إذا فيه تخفيف طبعاً، هيك ما حدث
+
+302
+00:24:05,800 --> 00:24:10,000
+تخفيف كيف بي .. بي .. بي .. بيجي الأسئلة على هذا
+
+303
+00:24:10,000 --> 00:24:16,380
+النوع من الـ dilution، أول حاجة في قانون الـ
+
+304
+00:24:16,380 --> 00:24:21,430
+dilution، طبعاً قانون الديلوشن هو عبارة عن الـ volume
+
+305
+00:24:21,430 --> 00:24:27,970
+of concentrated على
+
+306
+00:24:27,970 --> 00:24:30,310
+الـ total أو الـ final volume
+
+307
+00:24:34,450 --> 00:24:39,090
+طبعاً وهذا طبعاً القانون موجود معانا بالشرائح دي
+
+308
+00:24:39,090 --> 00:24:43,590
+وأنا جيت هنا حكيت أنّه الـ dilution represent the
+
+309
+00:24:43,590 --> 00:24:47,430
+ratio of concentrated of stock material of the
+
+310
+00:24:47,430 --> 00:24:52,550
+total final volume of solution يعني النسبة أو
+
+311
+00:24:52,550 --> 00:24:56,990
+النسبة يعني قسمة، not قسمة مين؟ الحجم الـ
+
+312
+00:24:56,990 --> 00:25:01,970
+concentrated على الـ final volume، على الـ total volume
+
+313
+00:25:03,590 --> 00:25:08,370
+فهذا هو القانون
+
+314
+00:25:08,370 --> 00:25:10,270
+الـ dilution
+
+315
+00:25:13,280 --> 00:25:17,420
+قانون الـ dilution هو الـ volume of concentrated على
+
+316
+00:25:17,420 --> 00:25:20,960
+الـ total volume، ده خلينا نعمل مسألة جزئية، هتحسب
+
+317
+00:25:20,960 --> 00:25:26,360
+إيش هو يعني الـ dilution؟ الـ volume of concentrated
+
+318
+00:25:26,360 --> 00:25:30,420
+أنا قد إيش أخدت من الـ stock، من الـ original؟  تبعين من
+
+319
+00:25:30,420 --> 00:25:35,700
+الـ مركز، أخذت واحد مل، إذا بحط واحد مل، طيب الـ total
+
+320
+00:25:35,700 --> 00:25:38,360
+volume قد إيش؟  صار في التيوب اللي مخفف، أخذنا عن الـ
+
+321
+00:25:38,360 --> 00:25:43,270
+volume عندي واحد زائد تسعة، طبعاً هو يساوي واحد على
+
+322
+00:25:43,270 --> 00:25:50,010
+عشرة، طبعاً واحد على عشرة هذا هو الـ .. الـ .. الـ
+
+323
+00:25:50,010 --> 00:25:53,490
+dilution تبع اليوبيا، إذا هذه اليوبيا مخففة عشر
+
+324
+00:25:53,490 --> 00:25:57,870
+مرات من الـ .. الـ original طبعاً تركيزها عشر الـ
+
+325
+00:25:57,870 --> 00:26:02,410
+original هي .. هي الـ .. الـ .. هي النسبة بالـ
+
+326
+00:26:02,410 --> 00:26:11,640
+dilution، طيب مثلاً لو كان عندي أنا الـ .. الـ .. الـ ..
+
+327
+00:26:11,640 --> 00:26:14,360
+المسألة هذه يقول لي أحسب التركيز هنا، أنا كده بدي
+
+328
+00:26:14,360 --> 00:26:19,080
+أحسبها، أخذنا قانون في التحليل  يساوي M V يساوي M
+
+329
+00:26:19,080 --> 00:26:25,600
+شارطة V شارطة، هذا ما قبل التخفيف طبعاً، وهذا بعد
+
+330
+00:26:25,600 --> 00:26:31,120
+التخفيف كان صح؟ و .. أو .. وموجود بصيغة نفس
+
+331
+00:26:31,120 --> 00:26:36,540
+القانون ولكن بس إحنا معبرين عنه بـ C V يساوي C V، C1
+
+332
+00:26:36,540 --> 00:26:43,180
+V1 قبل التخفيف وC2 V2 بعد التخفيف طبعاً؟ طيب C1
+
+333
+00:26:43,180 --> 00:26:49,580
+كم؟ كانت 200 بهذا المثال، V1 كانت .. لنفرض أن الـ V
+
+334
+00:26:49,580 --> 00:26:54,730
+مثلاً هي واحد مل، حجمها هنا طبعاً .. بس هنا ما بفيدني
+
+335
+00:26:54,730 --> 00:26:59,570
+.. أنا ما بفيدني قد إيش آخذ 8 من الـ .. منه .. آخذ منه
+
+336
+00:26:59,570 --> 00:27:03,530
+واحد عشان أخففه .. يعني ضارب واحد .. ماشي، ضارب واحد
+
+337
+00:27:03,530 --> 00:27:09,330
+هنا .. طبعاً الـ C2 قد إيش هو المجهول اللي مش معروفة ..
+
+338
+00:27:09,330 --> 00:27:15,060
+الـ V2 طبعاً الـ volume هنا إجمالي تسعة مل في الـ tube
+
+339
+00:27:15,060 --> 00:27:20,220
+هنا طبعاً واحد، تسعة، فهذه المسألة عوض فيها
+
+340
+00:27:20,220 --> 00:27:27,100
+فهنا مثلاً على عشرة على عشرة هنا على
+
+341
+00:27:27,100 --> 00:27:31,200
+عشرة بيروحوا، حضر مع بعض وبيطلع الـ concentration
+
+342
+00:27:31,200 --> 00:27:37,670
+C2 عشرين، طبعاً عشرين إيش؟ عشرين نفس التركيز
+
+343
+00:27:37,670 --> 00:27:42,410
+milligram بالأقل، milligram بالأقل طبعاً هذا هو
+
+344
+00:27:42,410 --> 00:27:50,470
+تركيز الـ .. الـ يوليّة، إذا لاحظتوا الأصل مئتين طبعاً و
+
+345
+00:27:50,470 --> 00:27:55,110
+المخففة عشرين والمخففة، فحكيت لكم إيش معناها؟ عشر
+
+346
+00:27:55,110 --> 00:28:01,910
+الأصل، المئتين، عشرين، طبعاً هي هنا الـ .. يعني حلو
+
+347
+00:28:01,910 --> 00:28:06,090
+الربط بين المعلومات مع بعضها البعض، طيب كيف تيجي
+
+348
+00:28:06,090 --> 00:28:11,850
+الأسئلة أيضاً على هذا الشكل؟ هي هنا مثلاً عندي مثال
+
+349
+00:28:11,850 --> 00:28:15,530
+بالملزمة
+
+350
+00:28:15,530 --> 00:28:18,690
+أو بالمحاضرة تبعتنا، هي
+
+351
+00:28:23,190 --> 00:28:28,170
+بدي أحضر 45 مل من محلول يكون تركيزه 30%
+
+352
+00:28:28,170 --> 00:28:33,150
+solution والـ stock تبعي من الأصل اللي هو 70%
+
+353
+00:28:33,150 --> 00:28:36,610
+تركيزه طبعاً، هذا كيف أنا بدي أطبقه، يعني هلّأ بدي
+
+354
+00:28:36,610 --> 00:28:42,590
+أطبقه، هي هنا بدي أحضر أنا 30% محلول و
+
+355
+00:28:42,590 --> 00:28:45,670
+الـ final volume يكون خمسة وأربعين، أنا بدي خمسة و
+
+356
+00:28:45,670 --> 00:28:48,750
+أربعين مل من المحلول اللي تركيزه تلاتين بالمئة
+
+357
+00:28:48,750 --> 00:28:52,630
+من الـ original عندي سبعين بالمئة، طب قد إيش آخذ
+
+358
+00:28:52,630 --> 00:28:58,410
+من الـ original؟ مش معروف، بروح على C1 V1 يساوي C2 V2
+
+359
+00:28:58,410 --> 00:29:04,580
+والله بعد التخفيف تركيزي يكون 30%، طبعاً يا بنات
+
+360
+00:29:04,580 --> 00:29:08,980
+بدنا ننوّه أنّ C1 يعني المقصود فيه concentration و
+
+361
+00:29:08,980 --> 00:29:14,780
+V1 volume one يعني قبل التخفيف، الـ initial وC2
+
+362
+00:29:14,780 --> 00:29:18,440
+طبعاً concentration وvolume بعد التخفيف، عشان هي
+
+363
+00:29:18,440 --> 00:29:22,080
+أعطيناها الرقم اثنين، نفس الفكرة M في V، الـ M
+
+364
+00:29:22,080 --> 00:29:26,400
+molarity يعني concentration ضرب V volume يساوي الـ
+
+365
+00:29:26,400 --> 00:29:30,960
+molarity في الـ volume يعني حاطينهم شرطة يعني بعد
+
+366
+00:29:30,960 --> 00:29:34,440
+التخفيف، هذه الفكرة بس عشان نفهم إيش يعني C وإيش
+
+367
+00:29:34,440 --> 00:29:37,460
+يعني V، C concentration في volume يساوي الـ
+
+368
+00:29:37,460 --> 00:29:41,140
+concentration في volume قبل التخفيف يساوي ما بعد
+
+369
+00:29:41,140 --> 00:29:45,820
+التخفيف، هذا القانون أصلاً المفروض هو موجود معاكم
+
+370
+00:29:45,820 --> 00:29:49,560
+بالـ تحليلية اللي هو عبارة عن number of moles يساوي
+
+371
+00:29:49,560 --> 00:29:53,240
+number of moles هذا بتاخذوه في التحليلية مع المعادلة
+
+372
+00:29:53,240 --> 00:29:56,920
+المهم أنتو تعرفوا أنّ C1 في V1، C يعني المقصود فيه
+
+373
+00:29:56,920 --> 00:30:02,550
+concentration وV1 المقصود فيه volume، C2
+
+374
+00:30:02,550 --> 00:30:06,130
+concentration وvolume ولكن بعد التخفيف، نفس فكرة
+
+375
+00:30:06,130 --> 00:30:10,650
+M وV، الـ M molarity يعني أصلاً concentration، إحنا
+
+376
+00:30:10,650 --> 00:30:15,150
+من شوية لسة شرحنا، حكينا أن الـ molarity أحد طرق
+
+377
+00:30:15,150 --> 00:30:18,190
+التعبير عن الـ concentration، الـ chemical unit تمام
+
+378
+00:30:18,190 --> 00:30:25,550
+فهي الفكرة، والحجم النهائي بتعطيه 45 وقبل التخفيف
+
+379
+00:30:25,550 --> 00:30:30,310
+أنا عندي 70 فمش معروف عندي الـ volume هذا قد إيش، فعلى
+
+380
+00:30:30,310 --> 00:30:36,410
+طول تعويض مباشر، تمام، فبقسم وبحسب بيطلع معايا
+
+381
+00:30:36,410 --> 00:30:42,750
+الحجم زي ما هو موجود في الـ slides 19.3 مل تمام
+
+382
+00:30:42,750 --> 00:30:48,170
+هذا بيجي هيك، طيب ممكن يجي أيضاً يجي عندي مثلاً
+
+383
+00:30:48,170 --> 00:30:54,990
+high etude، أخذنا منها حجم معيّن تمام بحيث أنه كانت
+
+384
+00:30:54,990 --> 00:30:59,290
+الـ .. الـ .. هنا تركيز 50% صار 25%
+
+385
+00:30:59,290 --> 00:31:04,890
+تمام؟ وهنا الحجم أو الـ volume
+
+386
+00:31:04,890 --> 00:31:11,370
+النهائي خمسة مل تمام؟ مش معروف قد إيش أنا أخذت من
+
+387
+00:31:11,370 --> 00:31:11,630
+هنا
+
+388
+00:31:19,050 --> 00:31:24,010
+طبعاً في هذا المثال معروفة الـ C1 تركيز الأول هو 50
+
+389
+00:31:24,010 --> 00:31:29,040
+%، الـ V1 أنا قد إيش أخذت هو اللي مجهول، C2 خمسة وعشرين
+
+390
+00:31:29,040 --> 00:31:33,880
+بالمئة معروفة وV2 اللي هو الـ final volume أو الـ
+
+391
+00:31:33,880 --> 00:31:38,560
+volume النهائي هي خمسة مل معروفة، يعني إذا V1
+
+392
+00:31:38,560 --> 00:31:41,220
+فقط هو اللي مجهول اللي هو الـ volume اللي أنا نقلته
+
+393
+00:31:41,220 --> 00:31:44,580
+عشان أعمل dilution تمام؟ هو اللي مش موجود أو مش
+
+39
+
+445
+00:36:34,320 --> 00:36:39,700
+النهائي كان عندي عشرة، خمسة، و خمسة عشر طبعاً
+
+446
+00:36:39,700 --> 00:36:48,780
+فإذا بعد هيك، بطبق طاقة تطبيق مباشر للحسابات
+
+447
+00:36:48,780 --> 00:36:50,280
+و بجيب اللي هو C2
+
+448
+00:36:54,910 --> 00:36:58,090
+طبعاً يا بنات، أنا لما بكتب القوانين، بكتبها
+
+449
+00:36:58,090 --> 00:37:04,950
+مختصرة لأنّه مش مجدٍ، بياخد وقت معايا، وأنا بحاول أن
+
+450
+00:37:04,950 --> 00:37:08,750
+أنا أكتب الـ قانون كامل، فـ volume of
+
+451
+00:37:08,750 --> 00:37:11,810
+concentrated اللي هي نفسها volume of sample أو
+
+452
+00:37:11,810 --> 00:37:15,310
+volume of concentrated solution، عادي، بس أنا بختصر
+
+453
+00:37:15,310 --> 00:37:20,350
+في الكتابة لتوفير الوقت فقط،  لغلبة وأنا بشرح
+
+454
+00:37:20,680 --> 00:37:24,280
+فهذا الشيء، يعني خذوه بعين الاعتبار، تركيزها ثلاثة
+
+455
+00:37:24,280 --> 00:37:29,480
+في المئة، ثلاثة في المئة بالحسابات، وبجيب اللي هو C2
+
+456
+00:37:29,480 --> 00:37:34,980
+كاتب لي في الـ slides أنّ الـ dilution عبارة عن ايه؟
+
+457
+00:37:34,980 --> 00:37:42,840
+عبارة عن الـ volume of sample على الـ total volume
+
+458
+00:37:42,840 --> 00:37:46,020
+طيب يا miss، أنتِ حكيتِ لي أنّ الـ volume of concentrated
+
+459
+00:37:46,020 --> 00:37:49,340
+هنا، طيب ايش يعني؟ ايش الفرق ما بين
+
+460
+00:37:53,600 --> 00:37:58,200
+طبعاً الفرق ما بينهم، طبعاً ما فيش فرق، سواء أنا حكيت
+
+461
+00:37:58,200 --> 00:38:01,480
+سواء كان عندي stock solution أو concentrated
+
+462
+00:38:01,480 --> 00:38:05,140
+solution، بدي أعمله تخفيف، أو سواء كان عندي sample
+
+463
+00:38:05,140 --> 00:38:10,070
+عينة أنا بدي أخففها وأشتغل عليها، نفس المعنى، يعني في
+
+464
+00:38:10,070 --> 00:38:13,350
+النهاية اللي بيكون في البسط هو الـ volume اللي
+
+465
+00:38:13,350 --> 00:38:16,850
+أخذته من المادة المركزة أو المادة المراد تخفيفها
+
+466
+00:38:16,850 --> 00:38:20,850
+سواء كانت محلول أو عينة، وعلى الـ final volume، جدش
+
+467
+00:38:20,850 --> 00:38:25,920
+صار في الحجم النهائي بعد ما ضفت الـ solvent عليها
+
+468
+00:38:25,920 --> 00:38:29,860
+الفكرة، أو هي هي، ده معنى قانون الـ dilution، تمام، و
+
+469
+00:38:29,860 --> 00:38:34,160
+احنا ذكرنا سابقاً في المحاضرة المسجلة أنّ أنا
+
+470
+00:38:34,160 --> 00:38:38,200
+أحياناً بضطر أخفف عينات لأنّ تركيزها بيكون عالي، و
+
+471
+00:38:38,200 --> 00:38:40,920
+الأجهزة اللي عندي، والمواد اللي عندي، قدرة
+
+472
+00:38:40,920 --> 00:38:43,880
+استيعابها ما بتقدرش تقيس للتركيز اللي موجودة في
+
+473
+00:38:43,880 --> 00:38:47,460
+العينة، وضربت لكم مثال، لما يكون السكر تركيزه 500
+
+474
+00:38:47,460 --> 00:38:54,500
+وعندي محلول بيقيس يعني، انكسر يعني ان، لأ يعني
+
+475
+00:38:54,500 --> 00:38:57,420
+على أقصى استيعاب عنده، بيقيس لحد ثلاثمائة
+
+476
+00:38:57,420 --> 00:39:00,960
+فإيش أعمل أنا بهي الحالة؟ بخفف العينة، طيب كيف بدي
+
+477
+00:39:00,960 --> 00:39:04,800
+أخفف العينة؟ بدي آخذ منها حجم، وأضيفه على
+
+478
+00:39:04,800 --> 00:39:08,300
+الـ sample، فأضيف حجم من الـ sample، وأضيف عليه
+
+479
+00:39:08,300 --> 00:39:11,410
+solvent، طبعاً كيف بدي أحسب الـ dilution للتخفيف
+
+480
+00:39:11,410 --> 00:39:14,830
+التخفيف اللي حصل هنا للعينة، جدش أنا أخدت من الـ
+
+481
+00:39:14,830 --> 00:39:18,750
+sample على حجم الـ sample مع الـ solvent كلهم سوا، الـ
+
+482
+00:39:18,750 --> 00:39:23,150
+total، هيك بكون طلعت الـ dilution، تمام، الجزء الآخر
+
+483
+00:39:23,150 --> 00:39:30,090
+من المحاضرة، لحظة، هي الجزء الآخر، أنا طبعاً صورتهم ف
+
+484
+00:39:30,090 --> 00:39:36,530
+هي اللي احنا بنكون مركزينها، يعني تم فصلهم لجزئين
+
+485
+00:39:36,530 --> 00:39:39,450
+يعني ما بعرف ليش انفصلوا، أو فصل التسجيل أثناء
+
+486
+00:39:39,450 --> 00:39:43,710
+تصويري، ما علينا، هذا هو الجزء الآخر، نستكمل من عند
+
+487
+00:39:43,710 --> 00:39:49,310
+القانون الـ dilution، القانون الثاني اللي كان متمليه
+
+488
+00:39:49,310 --> 00:39:52,230
+في الـ slides، أنّه volume of sample على الـ total
+
+489
+00:39:52,230 --> 00:39:55,450
+volume، هي هنا شرح وإعادة لنفس الكلام اللي ذكرته
+
+490
+00:39:55,450 --> 00:40:00,690
+من شوية، هي اللي احنا بنكون مركزينها، بتكون بدأنا نخففها
+
+491
+00:40:00,690 --> 00:40:03,870
+أو بتكون مركزة، هي نفس الفكرة، يعني volume of sample
+
+492
+00:40:03,870 --> 00:40:06,950
+أو volume concentrated، نفس الأشياء، على الـ total
+
+493
+00:40:06,950 --> 00:40:11,210
+volume، طيب لو أنا بدي أجيب هذه الـ dilution
+
+494
+00:40:11,210 --> 00:40:15,170
+تبعها، نص، لو بدي أجيب حالة اسمها dilution factor
+
+495
+00:40:15,170 --> 00:40:18,650
+ايش يعني؟ يعني مقلوب النص، مقلوب الـ dilution، الـ
+
+496
+00:40:18,650 --> 00:40:22,830
+dilution factor هو عبارة عن واحد على الـ dilution، و
+
+497
+00:40:22,830 --> 00:40:33,130
+يساوي واحد على الـ واحد على النص، يعني اثنين، مقلوب
+
+498
+00:40:33,130 --> 00:40:37,700
+الـ dilution، يعني اثنين، تمام، هذا هو الـ dilution
+
+499
+00:40:37,700 --> 00:40:40,480
+factor، بنرجع له كمان شوية لما أحكي عن الـ serial
+
+500
+00:40:40,480 --> 00:40:49,940
+dilution، طيب نيجي نحكي عن الـ serial dilutions، كيف
+
+501
+00:40:49,940 --> 00:40:54,020
+أنا بستخدم، بعمل الـ serial dilution، تمام، الـ
+
+502
+00:40:54,020 --> 00:40:56,680
+serial dilution، حاكية أنّه مثلاً لو كان عندي tube
+
+503
+00:40:56,680 --> 00:41:05,440
+تركيزين، تمام، molar، ومحلول مثلاً الـ glucose، تمام؟ و
+
+504
+00:41:05,440 --> 00:41:10,920
+أنا أخدت منه 1 ml وحطيتها في tube فيها 9 ml
+
+505
+00:41:10,920 --> 00:41:14,720
+distilled water، بعدين أخدت في tube ثانية 1 ml، و
+
+506
+00:41:14,720 --> 00:41:18,240
+حطيتها في tube فيها 9 ml من الـ distilled water
+
+507
+00:41:18,240 --> 00:41:21,960
+وأخذت في، في tube 5 ml، وحطيتها في tube كان
+
+508
+00:41:21,960 --> 00:41:26,190
+فيها 5 ml من الـ distilled water، أنا عندي الـ
+
+509
+00:41:26,190 --> 00:41:29,790
+tube رقم واحد، اثنين، ثلاثة، ثلاث قنابيب، أنا
+
+510
+00:41:29,790 --> 00:41:33,570
+عاملة لهم تخطيط، طيب لو أنا بدي آجي أعمل حسابات
+
+511
+00:41:33,570 --> 00:41:37,510
+لهي الثلاث قنابيب، أول حاجة بدي أحسب الـ dilution
+
+512
+00:41:37,510 --> 00:41:41,030
+لثلاث قنابيب، هو عبارة عن الـ volume لـ
+
+513
+00:41:41,030 --> 00:41:46,430
+concentrated على الـ total volume، صح؟ طيب الـ، كده
+
+514
+00:41:46,430 --> 00:41:50,490
+إيش أنا أخدت من الـ total، واحد، هي واحد على الـ total
+
+515
+00:41:50,490 --> 00:41:56,430
+واحد زائد تسعة يساوي عشرة، عشرة، طيب الـ tube هذه أنا
+
+516
+00:41:56,430 --> 00:41:59,350
+جدش أخدت من الـ، من اللي جاب، من الـ concentrated
+
+517
+00:41:59,350 --> 00:42:02,830
+يعني من اللي جابها المركزة وأكثر منها، أخدت واحد
+
+518
+00:42:02,830 --> 00:42:08,810
+طبعاً على واحد زائد تسعة، عشرة، بنفع أقول الـ tube هذه
+
+519
+00:42:08,810 --> 00:42:15,000
+تخفيفها عشرة، وأسكت، لأ، هذه تخفيفها عشرة من الأصل، لكن
+
+520
+00:42:15,000 --> 00:42:18,320
+هذه تخفيفها عشرة من الـ، من التيوب اللي جابها
+
+521
+00:42:18,320 --> 00:42:23,420
+اللي قبلها، تخفيفها عشرة، يعني أنّ التيوب رقم واحد، صح؟
+
+522
+00:42:23,420 --> 00:42:27,520
+تخفيفها طولها عشرة، واحد على عشرة، بس هي من الأصل
+
+523
+00:42:27,520 --> 00:42:32,430
+عشرة، واحدة، لو أنا جيت حكيت dilution of tube one
+
+524
+00:42:32,430 --> 00:42:37,490
+واحد على عشرة، و ساكتة، عادي، تمام؟ لأنّها، لأنّها
+
+525
+00:42:37,490 --> 00:42:41,430
+جاية من الأصل، لكن هي ما بنفع أحكي إنّ هي التيوب
+
+526
+00:42:41,430 --> 00:42:45,570
+تخفيفها أيضاً واحد لعشرة، لأ، هي تخفيفها واحد لعشرة
+
+527
+00:42:45,570 --> 00:42:50,160
+من التيوب اللي سبقتها، تمام؟ فما بنفع أحكي أنّ
+
+528
+00:42:50,160 --> 00:42:53,620
+تخفيفها واحد لعشرة وأسكت، لأ، بدي أكمل، بدي أحكي واحد
+
+529
+00:42:53,620 --> 00:42:57,980
+لعشرة من مين؟ من التيوب اللي سبقتها، اللي أصلاً أيضاً
+
+530
+00:42:57,980 --> 00:43:02,000
+تخفيفها واحد لعشرة، فأصبح بيصير تخفيف التيوب رقم
+
+531
+00:43:02,000 --> 00:43:05,620
+اثنين، واحد لعشرة من التيوب رقم واحد اللي تخفيفها
+
+532
+00:43:05,620 --> 00:43:09,500
+أيضاً واحد لعشرة، طيب كيف بدي أختصر هذا الكلام كله؟
+
+533
+00:43:09,500 --> 00:43:13,660
+بدي أقول واحد لعشرة ضرب واحد لعشرة اللي هنا، ويساوي
+
+534
+00:43:13,660 --> 00:43:19,400
+واحد على مئة، تخفيفها، هي معاكم الشريحة، حلو، بتشوفوا
+
+535
+00:43:19,400 --> 00:43:22,080
+كده، إذا أنا لو بدي أجيب الـ dilution اللي هي ده
+
+536
+00:43:22,080 --> 00:43:27,740
+المفروض أقول عشرة اللي هي جايه من العشرة اللي
+
+537
+00:43:27,740 --> 00:43:33,190
+جابتها، يعني عشرة في عشرة، ويساوي واحد على مئة، نضربهم في
+
+538
+00:43:33,190 --> 00:43:38,730
+بعض، طيب نفس الفكرة هنا، جدش الـ dilution، أخدت خمسة
+
+539
+00:43:38,730 --> 00:43:42,550
+من المركز اللي جابها، أو الأكثر تركيزاً منها، خمسة
+
+540
+00:43:42,550 --> 00:43:46,950
+على ايش؟ خمسة زائد خمسة، عشرة، يعني ايش؟ نص، ما بنفع
+
+541
+00:43:46,950 --> 00:43:53,390
+أقول نص وأسكت، لأ، نص من المخففة، نص من اللي جابها
+
+542
+00:43:54,070 --> 00:43:59,210
+طبعاً لو أنا قلت نص وأسكت، معناها أنّها مخففة نص من
+
+543
+00:43:59,210 --> 00:44:02,950
+الأصل، وهذا غلط، لأ، هي مخففة نص من اللي قبلها
+
+544
+00:44:02,950 --> 00:44:07,610
+يعني نص ضرب اللي قبلها، جدش؟ واحد على مئة، يساوي واحد
+
+545
+00:44:07,610 --> 00:44:08,630
+على مئة
+
+546
+00:44:10,980 --> 00:44:13,920
+الـ، الـ، الـ dilution، إذا هذه تخطيطها واحد لعشرة
+
+547
+00:44:13,920 --> 00:44:17,700
+تيوب رقم واحد، تيوب رقم اثنين واحد على مئة، تيوب رقم
+
+548
+00:44:17,700 --> 00:44:22,600
+ثلاثة واحد على ألفي، طيب لو أنا بدي أجيب التركيز، أنا
+
+549
+00:44:22,600 --> 00:44:26,220
+أنا عارفة أنّ الـ original تركيزها مئة، طيب جدش
+
+550
+00:44:26,220 --> 00:44:28,880
+بدي أجيب، لو بدي أجيب التركيز، عفواً، أنّ الـ
+
+551
+00:44:28,880 --> 00:44:31,840
+original تركيزها مئتان، هلأ أنا بدي أجيب تركيز
+
+552
+00:44:31,840 --> 00:44:36,900
+الأنابيب، كيف بدي أشتغل التركيز لكل تيوب؟ إيش بدي أعمل؟
+
+553
+00:44:36,900 --> 00:44:43,370
+التركيز لـ، of tube، concentration of a tube، تركيز
+
+554
+00:44:43,370 --> 00:44:50,210
+الأنابيب اللي عندي، يساوي الـ dilution of a tube
+
+555
+00:44:50,210 --> 00:44:56,390
+تمام، ضرب الـ original concentration
+
+556
+00:44:58,990 --> 00:45:02,870
+طيب إيش يعني هي الـ tube رقم واحد؟ الـ tube رقم واحد
+
+557
+00:45:02,870 --> 00:45:05,510
+الـ dilution of الـ tube، يعني الـ dilution of الـ tube
+
+558
+00:45:05,510 --> 00:45:11,350
+رقم واحد، جدش بيطلع؟ واحد على عشرة، طبعاً ضرب الـ
+
+559
+00:45:11,350 --> 00:45:14,190
+original concentration، جدش الـ original
+
+560
+00:45:14,190 --> 00:45:18,150
+concentration؟ مئتان، إذا بضرب في مئتان، وبده يطلع له
+
+561
+00:45:18,150 --> 00:45:27,460
+عشرون molar، طيب هي تخفيفها واحد على مئة، إذا الـ
+
+562
+00:45:27,460 --> 00:45:31,660
+dilution تبعها واحد على مئة، ضرب مئتان، ويساوي 2 molar
+
+563
+00:45:31,660 --> 00:45:38,140
+طيب هي تخفيفها واحد على ألفي، في مئتان، تركيز الـ
+
+564
+00:45:38,140 --> 00:45:43,220
+original concentration، ويساوي 1 molar، إذا أنا بقدر
+
+565
+00:45:43,220 --> 00:45:46,440
+من خلال هذا القانون، الـ concentration of a tube
+
+566
+00:45:46,440 --> 00:45:49,440
+أجيب تركيز القنابيب الثلاثة اللي حصلت عليهم في الـ
+
+567
+00:45:49,440 --> 00:45:55,560
+serial dilution، طيب على فرض، على فرض أنّ هذه
+
+568
+00:45:55,560 --> 00:45:59,920
+القيم، هذه القيم أعطاني إياها، هو أعطاني إياها
+
+569
+00:46:03,010 --> 00:46:09,390
+ولكن، ولكن أنّ هو أنّ الـ original اللي كان مجهول، مش
+
+570
+00:46:09,390 --> 00:46:12,190
+معروف، أنا مش عارفة الـ original هذا، جدش بدي
+
+571
+00:46:12,190 --> 00:46:16,090
+أحسب، بدي أحسبه، خلينا نشوف، ما هو يا إما بيعطيني
+
+572
+00:46:16,090 --> 00:46:19,010
+الـ original وبقول لي احسب الـ tube، الـ concentration
+
+573
+00:46:19,010 --> 00:46:21,830
+of الـ tube، يا إما بيعطيني الـ concentration of الـ
+
+574
+00:46:21,830 --> 00:46:25,110
+tube، وبقول لي احسب الـ original، هلأ أنا افترض أنّ
+
+575
+00:46:25,110 --> 00:46:28,610
+معاكي الـ concentration of الـ tube، وبدي تحسبي الـ
+
+576
+00:46:28,610 --> 00:46:34,750
+original، هي الـ original concentration يساوي الـ
+
+577
+00:46:34,750 --> 00:46:42,170
+dilution factor of الـ tube ضرب، ضرب، تمام، الـ
+
+578
+00:46:42,170 --> 00:46:49,970
+concentration of the same tube، إيش يعني؟ خلينا نطبق
+
+579
+00:46:49,970 --> 00:46:53,050
+الـ tube رقم واحد، الـ tube رقم واحد، قدش الـ dilution
+
+580
+00:46:53,050 --> 00:46:56,700
+factor؟ الـ dilution factor، احنا حكينا مطلوب الـ
+
+581
+00:46:56,700 --> 00:47:01,020
+dilution، يعني هنا الـ dilution واحد على عشرة، معناها
+
+582
+00:47:01,020 --> 00:47:05,980
+الـ dilution factor يساوي عشرة، تمام؟ يساوي عشرة، هنا
+
+583
+00:47:05,980 --> 00:47:12,840
+الـ dilution factor، فإذا الـ dilution factor عشرة، الـ
+
+584
+00:47:12,840 --> 00:47:15,620
+concentration of the same tube، قدش معطينيا إياها؟
+
+585
+00:47:15,620 --> 00:47:19,600
+نفترض أنّ معطينيا إياها عشرين، عشرة في عشرين، ويساوي مئتان
+
+586
+00:47:19,600 --> 00:47:25,040
+تمام، حساباتنا صح، طيب خلينا نطبق في الـ tube رقم
+
+587
+00:47:25,040 --> 00:47:28,580
+اثنين، الـ tube رقم اثنين، هنا الـ dilution factor
+
+588
+00:47:28,580 --> 00:47:32,640
+مقلوب الـ dilution، قدش؟ مئة، واحد على مئة، مقلوبها
+
+589
+00:47:32,640 --> 00:47:37,480
+مئة، مئة في اثنين، مئتان، الـ dilution factor لها دي مئتان
+
+590
+00:47:37,480 --> 00:47:42,780
+مقلوبها واحد على مئتان، طبعاً هي الـ dilution، الـ
+
+591
+00:47:42,780 --> 00:47:46,520
+dilution factor مئتان، مئتان في واحد، مئتان، إذا الـ
+
+592
+00:47:46,520 --> 00:47:49,880
+original بقدر أحسبه، أنّه هو عبارة عن الـ dilution
+
+593
+00:47:49,880 --> 00:47:53,340
+factor للي تيوب، ضرب التركيز نفس ليتيوب، ما بنفع أقول
+
+594
+00:47:53,340 --> 00:47:56,000
+والله الـ dilution factor لها دي ليتيوب عشرة، وأروح
+
+595
+00:47:56,000 --> 00:48:00,350
+أضربه في تركيز ليتيوب رقم اثنين، غلط، طبعاً لازم أنّ
+
+596
+00:48:00,350 --> 00:48:04,890
+أضربه في تركيز نفس الـ tube اللي هو عشرة في عشرين
+
+597
+00:48:04,890 --> 00:48:12,070
+مئتان، في اثنين، مئتان، في واحد، وهكذا، طبعاً، طيب نضرب
+
+598
+00:48:12,070 --> 00:48:18,310
+منها الآخر، وننهي فيه، علشان يكون معنى واضح كل شيء
+
+599
+00:48:18,310 --> 00:48:22,190
+أخذت عن الـ tube، طبعاً، طبعاً هذه الـ stock، أو قلنا
+
+600
+00:48:22,190 --> 00:48:30,360
+الـ original، طبعاً تركيزها مثلاً خمسمائة مليغرام
+
+601
+00:48:30,360 --> 00:48:36,380
+بالديسيلتر، أخذت منها إيش حكينا؟ واحد مل، تمام، و
+
+602
+00:48:36,380 --> 00:48:40,240
+عندي تسعة مل distilled water، وأخذت كمان أيضاً
+
+603
+00:48:40,240 --> 00:48:45,080
+واحد مل، وعندي تسعة مل distilled water، تمام
+
+604
+00:48:45,080 --> 00:48:49,760
+و، أو خليني أحكي مثلاً هنا أخذت اثنين مل، وفي
+
+605
+00:48:49,760 --> 00:48:54,200
+عندي ثمانية مل distilled water، وهنا مثلاً أخدت
+
+606
+00:48:54,200 --> 00:49:00,940
+خمسة مل، وفي عندي خمسة مل distilled water
+
+607
+00:49:01,360 --> 00:49:05,480
+طبعاً يلا نحسب الـ dilution للقنابيب الثلاثة، هي
+
+608
+00:49:05,480 --> 00:49:09,640
+عندي أنبوبة رقم واحد، أنبوبة رقم اثنين، أنبوبة رقم
+
+609
+00:49:09,640 --> 00:49:14,380
+ثلاثة، الـ dilution، قلنا الـ volume of concentrated
+
+610
+00:49:14,380 --> 00:49:18,300
+على الـ total volume، الـ concentrated على الـ total
+
+611
+00:49:18,300 --> 00:49:
+
+667
+00:53:47,740 --> 00:53:48,900
+هنا
+
+668
+00:53:59,300 --> 00:54:07,700
+عينة بكتيريا أخذت منها واحد مل و حطيتها على تسعة
+
+669
+00:54:07,700 --> 00:54:10,860
+مل يعني الـ total صار عشرة يعني الـ dilution اللي
+
+670
+00:54:10,860 --> 00:54:15,560
+هُدَا واحد على عشرة، تمام. طيب أخذت منها واحد مل
+
+671
+00:54:15,560 --> 00:54:18,640
+وكان في عندي تسعة مل distilled water يعني برضه
+
+672
+00:54:18,640 --> 00:54:22,540
+واحد على واحد زائد تسعة يعني واحد على عشرة من مين؟
+
+673
+00:54:22,760 --> 00:54:27,300
+من هذه، يعني واحد على عشرة ضرب واحد على عشرة يعني
+
+674
+00:54:27,300 --> 00:54:31,420
+واحد على مئة، تمام. طيب أخذنا من الواحد على مئة هذه
+
+675
+00:54:31,420 --> 00:54:35,880
+one ml وحطيتها على تسعة ml distilled water فصار
+
+676
+00:54:35,880 --> 00:54:39,660
+عندي تخفيفها واحد على عشرة من مين؟ من الواحد على
+
+677
+00:54:39,660 --> 00:54:43,280
+مئة يعني واحد على عشرة ضرب واحد على مئة يعني واحد
+
+678
+00:54:43,280 --> 00:54:48,280
+على ألف، إذا التخفيف، الـ dilution  إجماله واحد على
+
+679
+00:54:48,280 --> 00:54:52,230
+ألف يعني عشرة أس ثلاثة، والـ dilution factor ألف
+
+680
+00:54:52,230 --> 00:54:56,690
+طبعاً هنا في نقطة مهمة لازم إحنا نوّجه لها، أنّه لما
+
+681
+00:54:56,690 --> 00:55:02,010
+أجي أحكيلك احسبي الـ final dilution، إيش يعني final
+
+682
+00:55:02,010 --> 00:55:06,330
+dilution؟ مكتوب بالسلايدات أنّه الـ final dilution هو
+
+683
+00:55:06,330 --> 00:55:13,310
+حاصل ضرب تخفيفاتهم الثلاثة، أو هو تخفيف الأنبوبة
+
+684
+00:55:13,310 --> 00:55:19,800
+الأخيرة، يعني مثلاً هذه كانت واحد على تسعة عشر، وهاد
+
+685
+00:55:19,800 --> 00:55:25,560
+اثنين على ثمانية، تنقل، هي كانت يعني اثنين على عشرة
+
+686
+00:55:25,560 --> 00:55:31,140
+يعني خمس، وهاد كانت نص، تمام. لو جيت ضربت من نص
+
+687
+00:55:31,140 --> 00:55:34,960
+بالاثنين على عشرة، بالواحد على عشرة، بتطلع عندي اللي
+
+688
+00:55:34,960 --> 00:55:38,040
+هو تركيز تخفيف الأنبوبة الأخيرة اللي هو واحد
+
+689
+00:55:38,040 --> 00:55:41,440
+على مئة. طبعاً نفس الشغل اللي كنا نشتغله، مش أنا كنت
+
+690
+00:55:41,440 --> 00:55:44,460
+هنا طلعتها واحد على عشرة، بعدين هذه قلت اثنين على
+
+691
+00:55:44,460 --> 00:55:50,020
+عشرة يعني خمس، صح؟ هذه خمسة، خمس، طبعاً خمسة في الواحد
+
+692
+00:55:50,020 --> 00:55:55,320
+على عشرة، وهنا طلعت أنّه الـ.. وطلع عندي واحدة على
+
+693
+00:55:55,320 --> 00:55:59,240
+خمسين، وأنا طلعتها إنها نص، وضربتها بالواحدة على
+
+694
+00:55:59,240 --> 00:56:02,840
+خمسين، يعني سواء طلعت وقولت هذه عشرة، وهذه اثنين على
+
+695
+00:56:02,840 --> 00:56:06,620
+عشرة، وهذه نص، وضربتهم في بعض، أو اشتغلت الشغل هذا
+
+696
+00:56:06,620 --> 00:56:12,020
+لأنّه أنا طلع كل dilution لحال، نفس الشيء، المهم أنّه
+
+697
+00:56:12,020 --> 00:56:15,800
+أنا الـ final dilution هو تخفيف الأنبوبة الأخيرة، أو
+
+698
+00:56:15,800 --> 00:56:19,920
+حاصل ضرب التخفيفات اللي أنا بدي أحكي عنها، تمام؟ هذا
+
+699
+00:56:19,920 --> 00:56:26,880
+بالنسبة للـ Final Dilution، لما أجي أطلب أحكي الـ
+
+700
+00:56:26,880 --> 00:56:29,660
+Final Dilution عادي، هي بتعطيني تخفيف الأنبوبة
+
+701
+00:56:29,660 --> 00:56:34,060
+الأخيرة، يا إمّا بتقول لي هذه واحدة على عشرة، وسكتنا
+
+702
+00:56:34,060 --> 00:56:39,440
+وهذه اثنين على عشرة، وسكتنا، وهذه نص، وسكتنا، وبعدين
+
+703
+00:56:39,440 --> 00:56:44,520
+ضربناهم في بعض، طبعاً، أو بنقول واحد على عشرة، وبعدين
+
+704
+00:56:44,520 --> 00:56:47,720
+بنقول هذه اثنين على عشرة، وما نسكتش، وما نضربها في
+
+705
+00:56:47,720 --> 00:56:52,100
+الواحد على عشرة اللي قبلها، بعدين نقول إن هذه نص، و
+
+706
+00:56:52,100 --> 00:56:54,660
+بعدين ما نسكتش، ومضربها في الواحد على خمسين اللي
+
+707
+00:56:54,660 --> 00:56:58,660
+قبلها، وبتطلع لي واحد على مئة، وأقول إن هذا هو الـ
+
+708
+00:56:58,660 --> 00:57:02,280
+final conclusion، سواء اشتغلت هيك أو هيك، نفس الفكرة.
+
+709
+00:57:02,280 --> 00:57:11,650
+طبعاً إذا هيك بنكون خلّصنا المحاضرة تبع الـ solution
+
+710
+00:57:11,650 --> 00:57:14,730
+and dilution، وأتمنى أنّه يكون الشيء هذا ساعدكم
+
+711
+00:57:14,730 --> 00:57:19,570
+بالفهم، وطريقة الشرح على الورق قرّبت الشيء لأذهانكم
+
+712
+00:57:19,570 --> 00:57:25,170
+وخلت الوضع يعني يمكن تخيّله أو يمكن إدراكه، و
+
+713
+00:57:25,170 --> 00:57:26,030
+يعطيكم العافية.
+
+714
+00:57:30,330 --> 00:57:34,410
+طيب يا صبايا، هلأ المطلوب منكم أنّه المسائل اللي
+
+715
+00:57:34,410 --> 00:57:39,330
+موجودة بالمحاضرة، تمام؟ ترجعوا تحلوها وتشوفوا
+
+716
+00:57:39,330 --> 00:57:44,470
+تستفيدوا من الأفكار اللي موجودة فيها، تمام؟ وتشوفوا
+
+717
+00:57:44,470 --> 00:57:47,790
+حالكم أنتم هلأ فاهمين أو لأ، تعاملوا مع السؤال زي كأنه
+
+718
+00:57:47,790 --> 00:57:50,970
+الإجابة مش موجودة في الملزمة أو في الـ.. في الـ
+
+719
+00:57:50,970 --> 00:57:54,170
+power point، وحلّوا لحالكم، قيّموا نفسكم، شوفوا حالكم
+
+720
+00:57:54,170 --> 00:57:59,200
+فاهمين أو لأ، علشان محاضرة المناقشة على الـ Google
+
+721
+00:57:59,200 --> 00:58:02,960
+meet يوم السبت يكون فيها أنتم تناقشوني بالأشياء
+
+722
+00:58:02,960 --> 00:58:05,420
+اللي أنتم مش فاهمينها، أو اللي مش قادرين تفهموها.
+
+723
+00:58:05,420 --> 00:58:09,640
+طبعاً عشان أقدر أجاوبكم عليها، ونعالج الشيء أول
+
+724
+00:58:09,640 --> 00:58:13,920
+بأول الدراسة، أول بأول، والمراجعة، وإنّك أنت تثبت
+
+725
+00:58:13,920 --> 00:58:18,040
+المعلومات، هذا بفيدك أكثر، وإن شاء الله يعني هيك ما
+
+726
+00:58:18,040 --> 00:58:22,420
+بيكون شيء عليكم صعب، يعطيكم ألف عافية، وموفقين بإذن
+
+727
+00:58:22,420 --> 00:58:22,640
+الله
diff --git a/PL9fwy3NUQKwY9jl8xw-bWkVZkWcGGyspd/-PkMjabV1O4_postprocess.srt b/PL9fwy3NUQKwY9jl8xw-bWkVZkWcGGyspd/-PkMjabV1O4_postprocess.srt
new file mode 100644
index 0000000000000000000000000000000000000000..f93432a1b8b29ecfadb603278a301425eeeeebb3
--- /dev/null
+++ b/PL9fwy3NUQKwY9jl8xw-bWkVZkWcGGyspd/-PkMjabV1O4_postprocess.srt
@@ -0,0 +1,2908 @@
+1
+00:00:03,490 --> 00:00:07,770
+مرحبا صبايا هلأ احنا لما شرحنا محاضرة solution
+
+2
+00:00:07,770 --> 00:00:11,530
+used in medical laboratory كان فيه جزئية طبعا انه
+
+3
+00:00:11,530 --> 00:00:18,210
+هي بدها تفاعل و بدها شرح يعني على سبورة او على ورق
+
+4
+00:00:18,210 --> 00:00:23,330
+لحتى توضح الفكرة فانا حكيتلكم انه راح أصورلكم
+
+5
+00:00:23,330 --> 00:00:28,130
+محاضرة أخرى كملحق لإلها علشان انه تدرسوهم مع بعض و
+
+6
+00:00:28,130 --> 00:00:30,410
+توضح الفكرة خصوصا في جزئية dilution
+
+7
+00:00:41,500 --> 00:00:47,020
+طبعا أنا بدأت بال .. بالجزئية الأولى للمحاضرة يعني
+
+8
+00:00:47,020 --> 00:00:52,200
+حكيت عن .. عن المحاليل و أنواعها و شرحت بعض
+
+9
+00:00:52,200 --> 00:00:56,610
+الأفكار عنهاو بعدين حكينا عن الوحدات القياس و من
+
+10
+00:00:56,610 --> 00:01:01,270
+ثم ال dilution فبرضه هذه المحاضرة شاملة و يعني
+
+11
+00:01:01,270 --> 00:01:06,250
+المهم أنه تدرسوها جنب تسجيل المحاضرة الأساسي اللي
+
+12
+00:01:06,250 --> 00:01:09,810
+نزل على ال model طبعا هلأ أنا راح أبلش أشغل
+
+13
+00:01:09,810 --> 00:01:14,390
+الفيديو هيبدأ يعرضلكم الفيديو اللي مصور أصلا سابق
+
+14
+00:01:14,390 --> 00:01:18,750
+مسبقا وبيشرح المحاضرة السلام عليكم ورحمة الله
+
+15
+00:01:18,750 --> 00:01:23,920
+وبركاته طالبتيقليبة مستوى .. قليبة مستوى التاني
+
+16
+00:01:23,920 --> 00:01:28,200
+قصة معلم طبية مقبلية أهلا وسهلا بكم ان شاء الله
+
+17
+00:01:28,200 --> 00:01:34,480
+اللي رح اليوم نعطي تاكملة المحاضرة اللي حكينا رح
+
+18
+00:01:34,480 --> 00:01:38,780
+نشرحها على ورق مقدر بالأمثلة لحتى انعمق الفهم
+
+19
+00:01:38,780 --> 00:01:42,890
+للمحاضرة المسجلةكانت المحاضرة تنبيع منها solution
+
+20
+00:01:42,890 --> 00:01:46,450
+used in medical laboratories وحكينا في المحاضرة
+
+21
+00:01:46,450 --> 00:01:49,210
+أنه انا فيها من ياله بدرة أنواع من المحاليات
+
+22
+00:01:49,210 --> 00:01:53,170
+المستخدمة في المختبرات الطبية، من هذه المحاليات
+
+23
+00:01:53,170 --> 00:01:58,700
+الرئيسات أربع أنواعالصبغات البقهور و ال standard
+
+24
+00:01:58,700 --> 00:02:02,860
+حكينا عن ال reagent هو اللى بيعملى ال change طبعا
+
+25
+00:02:02,860 --> 00:02:06,180
+هو المادة المتفاعلة اللى بتضيقها على العينة بتعملى
+
+26
+00:02:06,180 --> 00:02:12,680
+change تغير measurable or noticable يعني أما يكون
+
+27
+00:02:12,680 --> 00:02:17,840
+يمكن قياسه أو يمكن ملاحظته هذا التغير اللى يحدثه
+
+28
+00:02:17,840 --> 00:02:21,740
+على العينة بنجرد أنه يفتح على العينة للكشف على
+
+29
+00:02:21,740 --> 00:02:27,310
+مادة معينة أو يعني يعنيعن جزء من العيلة مراقية او
+
+30
+00:02:27,310 --> 00:02:31,730
+مراد فحصه النوع الآخر من المحاليل كان هو الصبغات
+
+31
+00:02:31,730 --> 00:02:35,230
+النوع الثاني من المحاليل حكيناه اللي هو الصبغات
+
+32
+00:02:35,230 --> 00:02:40,310
+اللي بيعطيني مكون من طبعا من colored dyes اللي هي
+
+33
+00:02:40,310 --> 00:02:44,570
+صبغات ملونة تكسب العيلة artificial color او لون
+
+34
+00:02:44,570 --> 00:02:48,090
+مكتسب او لون استماعى لحتى يتمكن من رؤيتها تحت ال
+
+35
+00:02:48,090 --> 00:02:51,780
+microscopeالنوع التالت من المحاليل كان البحر اللي
+
+36
+00:02:51,780 --> 00:02:55,660
+هو المحلول الضعبة للبيئة هذا النوع من المحاليل
+
+37
+00:02:55,660 --> 00:02:59,800
+control بيئة هذا وظيفته ومن خصائصه ان هو resist
+
+38
+00:02:59,800 --> 00:03:04,400
+change البيئة بقالم التغير في البيئة النوع الرابع
+
+39
+00:03:04,400 --> 00:03:07,640
+اللي هو ال standard طبعا هحكيله مش بالترتيب زي ما
+
+40
+00:03:07,640 --> 00:03:12,700
+هم بالمحاضرة فيعني لأ بس لذكر ال standards هو
+
+41
+00:03:12,700 --> 00:03:16,840
+المحاليل القياسيةطبعا ال standard هي عبارة عن
+
+42
+00:03:16,840 --> 00:03:20,940
+محالي قياسية او ميارية او اهم ما يميزها انها non
+
+43
+00:03:20,940 --> 00:03:25,880
+-concentration طبعا non-concentration تاكيز معلوم
+
+44
+00:03:25,880 --> 00:03:31,040
+طبعا احنا حكينا انه استخدام ال standard solution
+
+45
+00:03:31,040 --> 00:03:36,120
+انه انا لان تاكيزها بيكون معلوم بجيب تاكيز مشهور
+
+46
+00:03:36,120 --> 00:03:41,640
+في العينة بدلت هذا المعلوم كيف يحدث هذا؟لو فرضت
+
+47
+00:03:41,640 --> 00:03:45,580
+انه انا في عندى عينة فيها سامل في .. طبعا النبو
+
+48
+00:03:45,580 --> 00:03:50,820
+فيها عينة و النبو اخر فيه محلول standard طبعا اللي
+
+49
+00:03:50,820 --> 00:03:53,740
+لو انا بدي اقس مستوى السكر في الدم بدي اقس ال
+
+50
+00:03:53,740 --> 00:03:57,700
+glucose اللي موجود في الدموهذا المحلول طبعا أكيد
+
+51
+00:03:57,700 --> 00:04:00,560
+هيكون محلول glucose معلوم التركيز عشان انا اقرأ
+
+52
+00:04:00,560 --> 00:04:04,340
+نوع العيلة مابنفعش مثلا انا اشأ بدي اقلس السكر
+
+53
+00:04:04,340 --> 00:04:07,660
+اجيب ال standard محلول البروتين لأ لازم يكون من
+
+54
+00:04:07,660 --> 00:04:12,220
+نفس النوع علشان انا استخدمه ك .. كمحلول معلوم
+
+55
+00:04:12,220 --> 00:04:17,300
+التركيز و اجيب المجهول بدلالتهطبعا هذا بالنسبة لمن
+
+56
+00:04:17,300 --> 00:04:22,700
+لا ال .. السامبل بدي ا .. ا .. اشوف ال .. بدي اقيس
+
+57
+00:04:22,700 --> 00:04:26,160
+تاركيز من ال glucose اللي موجود فيها و الاسفلين دي
+
+58
+00:04:26,160 --> 00:04:29,200
+محلول استعداد اقل موجود في ال glucose محلول اللي
+
+59
+00:04:29,200 --> 00:04:32,920
+هو معاهم التاركيز من ال glucose بدي انا من خلال ال
+
+60
+00:04:32,920 --> 00:04:40,410
+..التجربة أستخدمه كمحلول مياري أو تياسي أول اشي
+
+61
+00:04:40,410 --> 00:04:43,410
+بدي أعمله بدي أضيف ال reagent هو اللي هي المادة
+
+62
+00:04:43,410 --> 00:04:47,490
+المتفاعلة على كل مانع ال simple و ال standard راح
+
+63
+00:04:47,490 --> 00:04:50,850
+أضيف ال reagent المادة المتفاعلةطبعا المادة
+
+64
+00:04:50,850 --> 00:04:54,050
+المتفاعلة دهالى او اللى .. اللى راح تعطينى change
+
+65
+00:04:54,050 --> 00:04:58,070
+راح تعطينى مجارب organic metabolic change طبعا
+
+66
+00:04:58,070 --> 00:05:02,250
+هتتفاعل مع ال glucose و تعطينى تغير تغير ممكن يكون
+
+67
+00:05:02,250 --> 00:05:06,430
+فى اللون او كده احنا درمنا مثال انه اذا انا رفض
+
+68
+00:05:06,430 --> 00:05:11,670
+انه في ال .. في ال .. فرد تغير اللون و يعطى لي لون
+
+69
+00:05:11,670 --> 00:05:17,030
+pink مثلا لو مثلا هانا اعطانى لون pink تمام و هانا
+
+70
+00:05:17,030 --> 00:05:23,410
+اعطانى ايضا لون pink اخرعلى فرض أنه التكييز هنا
+
+71
+00:05:23,410 --> 00:05:27,330
+عبارة عن 100 milligram بالديسيلتر بالنسبة لل
+
+72
+00:05:27,330 --> 00:05:32,750
+standard تمام؟ 100 milligram بالديسيلتر و أنا دخلت
+
+73
+00:05:32,750 --> 00:05:35,590
+الإبعاد اللي متستخدم بالها دي و ال standard عادي
+
+74
+00:05:35,590 --> 00:05:39,590
+هذه تقيس الشد كلون على فرض أنه أعطاني الشد كلون
+
+75
+00:05:39,590 --> 00:05:44,090
+كانت اتنين و أنا أعطاني واحدطبعا إذا هنا شدت اللون
+
+76
+00:05:44,090 --> 00:05:48,730
+نصف شدت اللون لل standard معناه إيه التركيز إذا
+
+77
+00:05:48,730 --> 00:05:52,470
+كان هنا مياه معناه يكون التركيز هنا خمسين على فرض
+
+78
+00:05:52,470 --> 00:05:58,890
+أنه أعطاني .. على فرض أنه أعطاني شدت لون أقوى هنا
+
+79
+00:05:58,890 --> 00:06:04,660
+بينيطبعا ف .. و الجهاز قاسها على أساس أنها هي
+
+80
+00:06:04,660 --> 00:06:10,680
+أربعة طبعا و هنا أتنين فلنفرض أنه الأتنين كانت
+
+81
+00:06:10,680 --> 00:06:13,540
+تاركيزها مية طبعا الأتنين كانت تاركيزها مية
+
+82
+00:06:13,540 --> 00:06:16,100
+الأربعة كانت راح تكون تاركيزها .. راح تكون
+
+83
+00:06:16,100 --> 00:06:19,740
+تاركيزها متين، اللي هو الدقف فبالتالي بهذا الشكل
+
+84
+00:06:19,740 --> 00:06:24,640
+أو بهذا المبدأ أنا بجيب تاركيز المواد أو المكونات
+
+85
+00:06:24,640 --> 00:06:29,700
+للعينة طبعا المشهودةبالدالات معلوقة لو هو ال
+
+86
+00:06:29,700 --> 00:06:32,900
+standard، هاد .. هاد فكرة استخدام ال standard
+
+87
+00:06:32,900 --> 00:06:36,480
+solution في معرفة التركيز .. التركيز المشغولة،
+
+88
+00:06:36,480 --> 00:06:40,480
+طبعا، و هي دور ال agent، دور ال agent هو يكشف أنه
+
+89
+00:06:40,480 --> 00:06:44,900
+يعطيه change، يعطيه تغيرطبعا؟ ال .. ال .. ال ..
+
+90
+00:06:44,900 --> 00:06:48,260
+إذا هك خلصنا و شرحنا ال reagent و ال standard
+
+91
+00:06:48,260 --> 00:06:51,600
+أكتر، ال stem حاكينا أن هو عبارة عن صبغة غلطي
+
+92
+00:06:51,600 --> 00:06:56,020
+مكونة من color dye و بت .. تكسب ال .. ال .. مكونات
+
+93
+00:06:56,020 --> 00:06:59,720
+العينا artificial color لحتى نتمكن من رؤيتها تحت
+
+94
+00:06:59,720 --> 00:07:03,580
+ال microscope من خلال هذه الصبغات أنا بتمكن رؤية
+
+95
+00:07:03,580 --> 00:07:08,720
+العينات بشكل أضح و مكوناتها سواء كانت خلايا، أملح،
+
+96
+00:07:08,720 --> 00:07:15,070
+مايكروباتبكتيريا فطريات وفيليات كل أنواع المكونات
+
+97
+00:07:15,070 --> 00:07:18,150
+لدهر دهر الخلية ممكن سباطها و رؤيتها تحت ال
+
+98
+00:07:18,150 --> 00:07:22,250
+microscope بالنسبة للبفر هو عبارة عن محلول حكينا
+
+99
+00:07:22,250 --> 00:07:26,390
+بقى بتلالي أش يقوم تراير في اللي أش وحكينا أن هو
+
+100
+00:07:26,390 --> 00:07:32,710
+بدي يكون مكوّن من أش إما من weak acid مع ال strong
+
+101
+00:07:32,710 --> 00:07:39,350
+base تبعتهأو ال strong acid و ال weak base تبعته و
+
+102
+00:07:39,350 --> 00:07:43,810
+base فى المنظمة شرحلكم ان هو ممكن يكون مقوم من ال
+
+103
+00:07:43,810 --> 00:07:47,350
+weak acid و ال weak source تبعته هى نفس المعنى
+
+104
+00:07:47,350 --> 00:07:51,710
+خلينا نضرب مثلا اللى هى عندها C-H-D-C-O-A-S اللى
+
+105
+00:07:51,710 --> 00:07:56,790
+هو عبارة عن هذا acidic acid تمام؟ acidic acid ال
+
+106
+00:07:56,790 --> 00:08:01,510
+acidic acid هذا عبارة عن weak acid تمام؟ممكن انا
+
+107
+00:08:01,510 --> 00:08:06,830
+اكون بطار باستخدامه مع وجود ال CH3COO مالك اللى هو
+
+108
+00:08:06,830 --> 00:08:10,330
+الايونات التفارق تبعه الايونات التفارق تبعه هذا
+
+109
+00:08:10,330 --> 00:08:14,950
+بسميه acetate تمام و هو عبارة عن ملح و هو اللى
+
+110
+00:08:14,950 --> 00:08:20,660
+خلاص انه strong baseطبعا هو متأين بيكون له خاصة
+
+111
+00:08:20,660 --> 00:08:24,840
+strong base هدول المع بعض بيكونوا متبقار اذا اما
+
+112
+00:08:24,840 --> 00:08:28,060
+weak acid و strong base او weak acid و ال .. و ال
+
+113
+00:08:28,060 --> 00:08:31,760
+.. و ال konjugate base او weak acid و ال salt تبعه
+
+114
+00:08:31,760 --> 00:08:36,340
+نفس المعنى طبعا هذا بالنسبة للمعنى الحليب و
+
+115
+00:08:36,340 --> 00:08:41,680
+المكوناتطبعا احنا شريحنا بالنسبة لمحالين هذه ايضا
+
+116
+00:08:41,680 --> 00:08:44,880
+الاعتبارات اللي تتطرق طرحها أثناء استخدامها أو
+
+117
+00:08:44,880 --> 00:08:49,420
+أثناء تحضيرها ويبقى علومات عن كيفية الحفظ والخواص
+
+118
+00:08:49,420 --> 00:08:53,780
+اللي لازم تتوفر فيها علشان انا اعتبرها سواء كانت
+
+119
+00:08:53,780 --> 00:08:56,960
+the end او اعتبرها standard او اعتبرها بفر وهكذا
+
+120
+00:08:57,430 --> 00:09:01,410
+طيب بعد هيك حكينا .. طبعا حكينا عن أن هو ال
+
+121
+00:09:01,410 --> 00:09:04,330
+standard solution أنه primary standard و secondary
+
+122
+00:09:04,330 --> 00:09:09,210
+standard و في النهاية حكينا أنه المحليل هذه لما
+
+123
+00:09:09,210 --> 00:09:14,830
+أنا بدي أعبر عن التركيز تبعها لازم أنا أستخدم
+
+124
+00:09:14,830 --> 00:09:20,930
+التركيز أحكي ال concentration و ال unit تبعته طبعا
+
+125
+00:09:20,930 --> 00:09:25,130
+أحكي عن ال concentration و ال unitبنفعش مثلا أحكي
+
+126
+00:09:25,130 --> 00:09:28,510
+وضع المحلول، ضربنا مثلا في المحاضرة المسجلة، أقول
+
+127
+00:09:28,510 --> 00:09:32,250
+إن أنا بنفعش أنا أجيب محلول تركيزه خمسين و أقول
+
+128
+00:09:32,250 --> 00:09:37,330
+إنه خمسين أو بس، طب خمسين أيش؟ طب يعني .. طب يعني
+
+129
+00:09:37,330 --> 00:09:42,650
+أنا بيأفهم هو .. هو يعني بأي وحدة علشان أعرف مدى
+
+130
+00:09:42,650 --> 00:09:47,310
+قوة التركيز تبعه، فبالتالي إنه لازم أنا أعبر عن ال
+
+131
+00:09:47,310 --> 00:09:51,350
+.. ال .. المحلول ب .. ال concentration بالرقم و ال
+
+132
+00:09:51,350 --> 00:09:55,690
+unitطبعا وحاكينا أنه في عندي نوعين من ال units من
+
+133
+00:09:55,690 --> 00:10:01,070
+الوحدات تستخدم للتعبير عن ال concentration في نوع
+
+134
+00:10:01,070 --> 00:10:08,950
+اسمه physical طبعا physical units ونوع آخر بنسميه
+
+135
+00:10:08,950 --> 00:10:15,580
+اللي هو ال chemical unitsطبعا؟ طيب، ماشي بنحكيلها
+
+136
+00:10:15,580 --> 00:10:18,340
+على ال physical يعني نسمي اسمها وحدة كيزيائية يعني
+
+137
+00:10:18,340 --> 00:10:25,800
+بنعبر بالوزن مثلا بالجراح، الطول مثلا ال length
+
+138
+00:10:25,800 --> 00:10:26,580
+بال centimeter
+
+139
+00:10:29,290 --> 00:10:33,810
+الحجم بالأقل هذه الوحدات النهائية اللي أنتوا
+
+140
+00:10:33,810 --> 00:10:38,050
+أخدتوها أكيد يعني أعتقد من طول مرات معاكم في مصارخ
+
+141
+00:10:38,050 --> 00:10:43,410
+الفيزيا فطبعا لو في عندي ثلاث أنواع من الوحدات
+
+142
+00:10:43,410 --> 00:10:50,110
+اللي هم weight to volumeو weight to weight او
+
+143
+00:10:50,110 --> 00:10:55,150
+weight per volume weight per weight و volume per
+
+144
+00:10:55,150 --> 00:10:59,030
+volume انا بحكيها weight to volume ratio نفس
+
+145
+00:10:59,030 --> 00:11:01,390
+الكلام يعني لو هو weight to volume او weight per
+
+146
+00:11:01,390 --> 00:11:05,070
+volume نفس المعنى طيب مفروض ان فيلم دي محلول مثلا
+
+147
+00:11:05,070 --> 00:11:10,340
+هذا المحلول مثلا AACLطبعا محلول مدح الطعام ال NACL
+
+148
+00:11:10,340 --> 00:11:14,580
+هذا تتيزه تلاتين في المية طبعا خلينا نشوف ايش معنى
+
+149
+00:11:14,580 --> 00:11:18,040
+كلمة تلاتين في المية طبعا هو موجود في دست البوتر
+
+150
+00:11:18,040 --> 00:11:24,680
+ال solvent طبعا طيب محلول NACL ال solute عندي NACL
+
+151
+00:11:24,680 --> 00:11:28,800
+طبعا و ال solventالـ distilled water احنا حاكينا
+
+152
+00:11:28,800 --> 00:11:32,360
+انه اي محلول بيكون مكوّن من solute تمام اللي هي
+
+153
+00:11:32,360 --> 00:11:36,800
+المادة المدابة و ال solvent المضيب تمام ال IACA هي
+
+154
+00:11:36,800 --> 00:11:40,500
+المادة المدابة و ال solvent ال distilled water هو
+
+155
+00:11:40,500 --> 00:11:44,340
+المضيب خليني نحكي انه تركيزه تلاتين تلمية خليني
+
+156
+00:11:44,340 --> 00:11:49,060
+نشوف ايش يعني تلاتين تلمية لو تلاتين تلمية لو كانت
+
+157
+00:11:49,060 --> 00:11:55,700
+الوحدة wet volume لو كانت الوحدة تلاتين تلمية wet
+
+158
+00:11:55,700 --> 00:12:02,380
+بال volumeطبعا من ال .. من ال NACL معناها أنا
+
+159
+00:12:02,380 --> 00:12:08,500
+استخدمت تلاتين جرام طبعا هذا .. هذا solute و هذا
+
+160
+00:12:08,500 --> 00:12:14,360
+solvent بيكون طبعا أنا استخدمت تلاتين جرام من ال
+
+161
+00:12:14,360 --> 00:12:20,300
+solute اللي هو H هو عبارة عن HNACL تلاتين جرام
+
+162
+00:12:20,300 --> 00:12:29,230
+طبعا لإنهاءاتطبعا من ال NACL و أخدت كمان مية AML
+
+163
+00:12:29,230 --> 00:12:35,410
+AML لإنفوريا منين؟ من الصولة اللي هو عبارة عن H
+
+164
+00:12:35,410 --> 00:12:40,320
+distilled waterهذا كان في حال لو أنا ساخدم تلاتين
+
+165
+00:12:40,320 --> 00:12:46,240
+كمية weight و volume NACL طيب لو كانت عندي تلاتين
+
+166
+00:12:46,240 --> 00:12:51,080
+كمية weight per volume او sorry weight per weight
+
+167
+00:12:51,080 --> 00:12:57,400
+طبعا NACL اشنا نعمل معناها طبعا احنا عارفين ان هذا
+
+168
+00:12:57,400 --> 00:13:04,350
+لل solute و هذا لل solvent طبعاتلاتين قاش اللويات
+
+169
+00:13:04,350 --> 00:13:10,990
+جرام على سبعين جرام هما ب .. هما بحط تمية كاملة زي
+
+170
+00:13:10,990 --> 00:13:15,210
+اللي ما هي لأ هما بكمل تكميل بحث أنه يكون ال total
+
+171
+00:13:15,210 --> 00:13:20,550
+تمام عندي مية اللي هي أنا مو نجيب تمية هذه هي مية
+
+172
+00:13:20,550 --> 00:13:25,000
+تمية تمام؟ انا من النسبة المقلوية اللي هناهذا
+
+173
+00:13:25,000 --> 00:13:28,860
+معناها انه لما اقول تلاتين في المية wet و بقى bare
+
+174
+00:13:28,860 --> 00:13:34,780
+wet لما احكي تلاتين في المية .. لما احكي تلاتين في
+
+175
+00:13:34,780 --> 00:13:40,540
+المية طبعا wet او sorry volume .. bare volume ايش
+
+176
+00:13:40,540 --> 00:13:46,300
+معناه هذا للsolute تلاتين ml طبعا و هذا لل solvent
+
+177
+00:13:47,500 --> 00:13:52,440
+70 ml اكمل التكميل بحكيتلكم في المحاضرة المسجلة
+
+178
+00:13:52,440 --> 00:13:56,340
+انه لما يكون الوحدات مختلفة انا بحط ال 30 زي ما هي
+
+179
+00:13:56,340 --> 00:13:59,800
+و ال 100 زي ما هي لما يكون الوحدات متشابهة weight
+
+180
+00:13:59,800 --> 00:14:02,460
+per weight volume per volume انا بكمل التكميل
+
+181
+00:14:02,460 --> 00:14:08,000
+لانهية يعني 30 gram هيكون من ال NACL 70 gram و ليس
+
+182
+00:14:08,000 --> 00:14:11,280
+ml من ال distilled water لإن انا بحكي weight per
+
+183
+00:14:11,280 --> 00:14:16,540
+weight لما احكي volume per volume بدي اقول 30 mlمن
+
+184
+00:14:16,540 --> 00:14:21,500
+الان محلول NACL و سبعين ML من السيوت اللي بتقوله
+
+185
+00:14:21,500 --> 00:14:26,220
+distilled water هذا معناه ان السيوت اللي انا
+
+186
+00:14:26,220 --> 00:14:30,480
+بستخدمه ممكن يكون مادة بودر او زيتها تمام ممكن
+
+187
+00:14:30,480 --> 00:14:34,220
+يكون عبارة عن محلول بدقة خفيفةطبعا solution بدي
+
+188
+00:14:34,220 --> 00:14:39,220
+أخففها طبعا فإذا هي زينا أخذت تلاتين أقل من ACL
+
+189
+00:14:39,220 --> 00:14:43,200
+هتقول طب ال NACL محتاجة كده أوي انه تلاتين أقل
+
+190
+00:14:43,200 --> 00:14:46,400
+بيكون البرأة محلول وانا بدي أكمله لسبعين عشان أصل
+
+191
+00:14:46,400 --> 00:14:51,720
+لتركيز تلاتين تلمية بدي أخففطيب بالنسبة لـ
+
+192
+00:14:51,720 --> 00:14:54,760
+chemical units حكي ان انا بعبر بالspecific gravity
+
+193
+00:14:54,760 --> 00:14:58,820
+و ممكن اعبر بواحد تانية اللي هم ال molarity و ال
+
+194
+00:14:58,820 --> 00:15:02,240
+normality ال molarity عبارة عن ايه في ال molarity
+
+195
+00:15:02,240 --> 00:15:08,580
+قانونها عبارة عن number of moles على ال volume per
+
+196
+00:15:08,580 --> 00:15:13,380
+liter خليني اختار ان انا مثلا عندي اتنين إجراء من
+
+197
+00:15:13,380 --> 00:15:18,500
+ال S2 و S04 المولارتي بتاعتهم جديش بتاعتهم تمام؟
+
+198
+00:15:18,640 --> 00:15:23,760
+خلّينا نشوف هنا اتنين جرام من الـ H2O4 كم الولادة
+
+199
+00:15:23,760 --> 00:15:27,140
+تبعتها بتطمون طبعا ال number of moles هنا يا بنات
+
+200
+00:15:27,140 --> 00:15:30,640
+عبارة عن إيش؟ عبارة عن ال weight على الملوك اللي
+
+201
+00:15:30,640 --> 00:15:37,320
+لرويت طبعا؟ طيب اتنين جرام على الملوك اللي لرويت
+
+202
+00:15:37,320 --> 00:15:43,120
+للـ H2O4 98 سنة جدا نحسبهاعشان اشتغل الوحدة اتنين
+
+203
+00:15:43,120 --> 00:15:47,120
+على تمانية وتسعين هذه ال number of moles على ال
+
+204
+00:15:47,120 --> 00:15:49,960
+volume ب one liter طبعا محدد ب one liter انا اخترت
+
+205
+00:15:49,960 --> 00:15:56,420
+على one liter بحسب جديش ال morality تبعتهاللي
+
+206
+00:15:56,420 --> 00:16:00,440
+بتطلع معايا طبعا إذا ال molarity بنعيدها تاني
+
+207
+00:16:00,440 --> 00:16:07,160
+تساوي number of moles على volume بالliter حاجة
+
+208
+00:16:07,160 --> 00:16:11,280
+بالliter طبعا ويساوي ال number of moles هذه إيش
+
+209
+00:16:11,280 --> 00:16:15,300
+بتساوي بتساوي ال weight على ال molecule ال weight
+
+210
+00:16:15,300 --> 00:16:22,050
+على كله طبعا هذا هو كله على ال volume بالliterكانت
+
+211
+00:16:22,050 --> 00:16:27,650
+عندي وقص وقص وفور و وزنتها جدش و اتنين جرام تمام
+
+212
+00:16:27,650 --> 00:16:32,290
+اتنين جرام على اللي مالك فيه قاروة جدش و وزنتها
+
+213
+00:16:32,290 --> 00:16:38,680
+تمانية و تسعين طبعا جرام برمولمش تملك الارواح على
+
+214
+00:16:38,680 --> 00:16:43,600
+ال volume per liter one liter فبحسبها جدش تطلع
+
+215
+00:16:43,600 --> 00:16:48,440
+عندى ال .. ال .. ال .. ال molarity طبعا هذا
+
+216
+00:16:48,440 --> 00:16:51,760
+بالنسبالى ال molarity طب ال normality ال normality
+
+217
+00:16:51,760 --> 00:16:55,620
+ماتيكيها بال slide عبارة عن ايش تملكي الارواح على
+
+218
+00:16:55,620 --> 00:17:00,620
+ال N ال N هنا هي عبارة عن ال equivalence ال
+
+219
+00:17:00,620 --> 00:17:06,050
+equivalence طبعا اشي عن equivalence هو تكافأةطبعا
+
+220
+00:17:06,050 --> 00:17:13,170
+يعني مثلا الأس و أس أخر لما بيتأين اش بيعطيني
+
+221
+00:17:13,170 --> 00:17:18,710
+بيعطيني أس أخر ساعة اتنين تكاعف أتنين إذا ال .. ال
+
+222
+00:17:18,710 --> 00:17:21,170
+.. ال normality لها ده المحلول هتكون ال molecular
+
+223
+00:17:21,170 --> 00:17:24,850
+weight تبعين وتسعين على اتنين و يساو تسعة و أربعين
+
+224
+00:17:26,410 --> 00:17:31,710
+طبعا ايش الوحدات هنا؟ بتطلع هنا بال normal انت
+
+225
+00:17:31,710 --> 00:17:35,170
+تنسى و قدرين normal هنا ال .. ال .. ال molarity
+
+226
+00:17:35,170 --> 00:17:39,650
+لما انا احسبها بتطلع عندى العرض و وحدة القياس
+
+227
+00:17:39,650 --> 00:17:44,430
+molar طبعا لإنها molarity هذه هي ال chemical units
+
+228
+00:17:44,430 --> 00:17:51,310
+اذا حكينا عن المحارير اللى بتستخدم في ال ..في
+
+229
+00:17:51,310 --> 00:17:57,190
+المقتورات الطبية وحكينا كيف انا بقارن ال .. بجيب
+
+230
+00:17:57,190 --> 00:18:00,170
+التركيز المجرور في العينة بدلالة ال standard ايش
+
+231
+00:18:00,170 --> 00:18:05,410
+الفكرة بالظبط وحكينا ايضا عن الوحدات اللي هي ال
+
+232
+00:18:05,410 --> 00:18:09,470
+physical units و ال chemical units و التطبيق عليها
+
+233
+00:18:09,470 --> 00:18:14,090
+تمام؟ بعد هي انتقلنا لحتى نحكي عن ال dilution
+
+234
+00:18:14,090 --> 00:18:19,410
+تمام؟ في المحاضرة المسجلة ال dilution التخليف
+
+235
+00:18:19,980 --> 00:18:24,480
+دالبتلكم مثال حكيتلكم انه لو انتي عندك كوب حطيتي
+
+236
+00:18:24,480 --> 00:18:30,140
+فيه معلق السكر طبعا خلينا نفترض انك انتي ضختي معلق
+
+237
+00:18:30,140 --> 00:18:35,300
+السكر هنا و حطيتيها و
+
+238
+00:18:35,300 --> 00:18:47,600
+عبيتي لنصها ماء طبعا فهنا سكر sugar حطيه هنا طيبو
+
+239
+00:18:47,600 --> 00:18:51,620
+احنا حاطينا نفس المعالقة نفس الكمية و انا عبيتها
+
+240
+00:18:51,620 --> 00:18:59,020
+لآخر ماء طبعا اذا .. اذا ال solute دي اسمها عبارة
+
+241
+00:18:59,020 --> 00:19:05,380
+عن sugar و ال solvent من distilled water بس انا
+
+242
+00:19:05,380 --> 00:19:11,840
+عبيت لنص و انا عبيت لكل الكاسة فاليامين رح يكون
+
+243
+00:19:13,740 --> 00:19:18,460
+طبعا اكيد هذا طبعا هيكون اللي هيدي sweet اكتر
+
+244
+00:19:18,460 --> 00:19:23,340
+sweet او اللي هيدي more sweet هذا لسه sweet هيكون
+
+245
+00:19:23,340 --> 00:19:24,060
+صح؟
+
+246
+00:19:26,640 --> 00:19:32,800
+ليش؟ لإن أنا هتضفت من ال solvent أكتر، هتخفف أكتر
+
+247
+00:19:32,800 --> 00:19:40,740
+طيب أصبح التخفيف هو عبارة عن إن أنا أقلل من ال
+
+248
+00:19:40,740 --> 00:19:45,040
+concentration من خلال إن أنا إيش أعمل؟ أضيف
+
+249
+00:19:45,040 --> 00:19:49,620
+solvent وهذا هو التعريف اللي موجود في الفيديو هو
+
+250
+00:19:49,620 --> 00:19:55,800
+إن أنا أعمل decrease لال concentration من خلال ان
+
+251
+00:19:55,800 --> 00:20:01,140
+انا addition اعمل addition لانهي ضعف المزيد من ال
+
+252
+00:20:01,140 --> 00:20:05,320
+solvent هذا تعريف ال dilution طبعا وحاكينا ان ال
+
+253
+00:20:05,320 --> 00:20:09,620
+dilution يستخدم كتير في المختبرات التدبية في تحديد
+
+254
+00:20:09,620 --> 00:20:15,390
+المحاليل فلازم احنا نستوعب فكرة انهحكينا ان انا في
+
+255
+00:20:15,390 --> 00:20:20,790
+عندي نوعين من ال dilution نوع بنسميه simple
+
+256
+00:20:20,790 --> 00:20:26,390
+dilution، dilution بسيط و نوع اخر بنسميه ال
+
+257
+00:20:26,390 --> 00:20:32,510
+dilution ال cereal، ال cereal dilution إيش يعني
+
+258
+00:20:32,510 --> 00:20:38,810
+simple dilution؟ يعني بخطوة واحدة بيصير عدة
+
+259
+00:20:38,810 --> 00:20:40,370
+multiple
+
+260
+00:20:44,230 --> 00:20:48,650
+steps و طبعا موجودة كلمة multiplier بالصلاة ده
+
+261
+00:20:48,650 --> 00:20:56,390
+multiplicator progressive steps طبعا هذه عدة خطوات
+
+262
+00:20:56,390 --> 00:20:59,410
+مع
+
+263
+00:20:59,410 --> 00:21:03,230
+السلامة يا عزيزي يا عزيزي يا عزيزيعدد خطوات طبعا
+
+264
+00:21:03,230 --> 00:21:08,030
+هذا الفرق ما بين اثنين بالوثيوب يعني طيب اش يعني
+
+265
+00:21:08,030 --> 00:21:11,850
+يعني مثلا لما انا يكون في عندي مادة بسميها خلينا
+
+266
+00:21:11,850 --> 00:21:15,510
+نسميها stock او اسميها original المادة الأصلية
+
+267
+00:21:15,510 --> 00:21:20,510
+اللي انا بده اخففها لو انا أخدت منها volume طبعا
+
+268
+00:21:20,510 --> 00:21:26,170
+وحطيته في ال tube تبعتي طبعا واضغطت هنا solvent
+
+269
+00:21:26,170 --> 00:21:32,990
+ماعرفش انا بعد ما أبقى عافيةعدة خطوات طبعا هذا
+
+270
+00:21:32,990 --> 00:21:38,470
+الفرق ما بين سنة بالوسيلية طيب اش يعني يعني مثلا
+
+271
+00:21:38,470 --> 00:21:41,430
+لما انا يكون في نعدي مادة بسميها خلينا نسميها
+
+272
+00:21:41,430 --> 00:21:44,970
+stock او بسميها original المادة الأصلية اللي انا
+
+273
+00:21:44,970 --> 00:21:50,710
+بده اخففها لو انا أخدت منها volume طبعا وحاطلته في
+
+274
+00:21:50,710 --> 00:21:58,420
+ال tube تبعتي طبعا واضفت هنا solventمعناها انا هنا
+
+275
+00:21:58,420 --> 00:22:01,760
+بخطوة واحدة يعني ان انا بصي .. نقلت منها لتيوب و
+
+276
+00:22:01,760 --> 00:22:06,200
+نقلت solvent هذا بسميه simple لكن ال serial ان انا
+
+277
+00:22:06,200 --> 00:22:12,500
+هيكون .. يكون عندي stock تمام؟ و انقل من خط .. منه
+
+278
+00:22:12,500 --> 00:22:16,680
+لتيوب و منه ايضا لتيوب اخرى و هكذا لتيوب اخرى
+
+279
+00:22:16,680 --> 00:22:20,940
+فيكون متسامسل يعني معقد اكتر فهذا بسميه serial
+
+280
+00:22:22,130 --> 00:22:28,070
+حكينا معلومة انه ال serial بيبلش ك simple يعني أول
+
+281
+00:22:28,070 --> 00:22:32,950
+خطوة في ال serial هي عبارة عن simple طبعا اول خطوة
+
+282
+00:22:32,950 --> 00:22:39,330
+في ال serial عبارة عن simple بعدين بكمل كل ما
+
+283
+00:22:39,330 --> 00:22:43,130
+اتعقد اكتر بقلط ال simple ال serial اشماله بيصير
+
+284
+00:22:43,130 --> 00:22:46,890
+ال serial و هكذا هاي الفرق ما بين simple dilution
+
+285
+00:22:46,890 --> 00:22:50,450
+و simple serial dilutionهلأ بناخد قمتلة على كل من
+
+286
+00:22:50,450 --> 00:22:54,550
+ال simple dilution و ال serial dilution لحتى نعرف
+
+287
+00:22:54,550 --> 00:22:59,330
+كيف احنا نحل المسائل المتعلقة بهذا ال .. ال ..
+
+288
+00:22:59,330 --> 00:23:04,570
+الشيء ده خلينا نحكي عن اول اشي ال simple dilution
+
+289
+00:23:04,570 --> 00:23:10,010
+simple
+
+290
+00:23:10,010 --> 00:23:10,570
+dilution
+
+291
+00:23:13,350 --> 00:23:18,150
+حكينا ان هو يحدث او يجري بخطوة واحدة يعني مثلا
+
+292
+00:23:18,150 --> 00:23:22,110
+نفرض ان انا في مدينة هناك يوب تركيزها مثلا نتين
+
+293
+00:23:22,110 --> 00:23:29,990
+ميلي جرام بار او قلب تمام؟ فيها محلول يولية تمام؟
+
+294
+00:23:29,990 --> 00:23:34,710
+مهدأ اسمها يولية لو انا بدي اخد منها من هناone
+
+295
+00:23:34,710 --> 00:23:39,750
+أبقال و احطها في تيوب و تيوبها بتاعة تسعة أبقال من
+
+296
+00:23:39,750 --> 00:23:42,790
+ال salient اللى نعتقد او نعتقد ان هو distilled
+
+297
+00:23:42,790 --> 00:23:49,630
+water اذا واحد أبقال من المحلول اللى تركيزوا متين
+
+298
+00:23:50,040 --> 00:23:54,240
+طبعا هذا الواحد الأمقالي اللى كان تحكيزه 200 انا
+
+299
+00:23:54,240 --> 00:23:58,240
+دى حطيته وضبط عليه 9 أضعاف الكيمية من ال distill
+
+300
+00:23:58,240 --> 00:24:02,040
+water يعني حطيته واحد أمقاله وحطيت عليه 9 كمان من
+
+301
+00:24:02,040 --> 00:24:05,800
+ال distill water اذا فهى فيه تخفيف طبعا هى ما حدث
+
+302
+00:24:05,800 --> 00:24:10,000
+تخفيف كيف بي .. بي .. بي .. بيجي الأسئلة على هذا
+
+303
+00:24:10,000 --> 00:24:16,380
+النبط من النوع من ال dilution اول حاجة في قانون ال
+
+304
+00:24:16,380 --> 00:24:21,430
+dilution طبعاقانون الديوشن هو عبارة عن ال volume
+
+305
+00:24:21,430 --> 00:24:27,970
+of concentrated على
+
+306
+00:24:27,970 --> 00:24:30,310
+ال total أو ال final volume
+
+307
+00:24:34,450 --> 00:24:39,090
+طبعا و هذا طبعا القانون موجود معانا بالصلاحيات ده
+
+308
+00:24:39,090 --> 00:24:43,590
+و أنا جيت هنا حكى انه ال dilution represent the
+
+309
+00:24:43,590 --> 00:24:47,430
+ratio of concentrated of stick material of the
+
+310
+00:24:47,430 --> 00:24:52,550
+total final volume of solution يعني الناس معدل او
+
+311
+00:24:52,550 --> 00:24:56,990
+بالنسبة يعني قسمة not قسمة مين الحجم ال
+
+312
+00:24:56,990 --> 00:25:01,970
+concentrated لاش لال final volume على total volume
+
+313
+00:25:03,590 --> 00:25:08,370
+فهذا هو القانون
+
+314
+00:25:08,370 --> 00:25:10,270
+ال delution
+
+315
+00:25:13,280 --> 00:25:17,420
+قانون ال dilution هو ال volume of concentrated على
+
+316
+00:25:17,420 --> 00:25:20,960
+ال total volume ده خلينا نعمل مسألة جزئية هتحسب
+
+317
+00:25:20,960 --> 00:25:26,360
+إيش هو يعني ال dilution ال volume of concentrated
+
+318
+00:25:26,360 --> 00:25:30,420
+أنا كدش أخدت من ال stock من ال original تبعين من
+
+319
+00:25:30,420 --> 00:25:35,700
+ال مركز أخدت one أقل إذا بحط one أقل طيب ال total
+
+320
+00:25:35,700 --> 00:25:38,360
+volume كدش سعر في اليوتيوبة اللي مخفف فخرا عن ال
+
+321
+00:25:38,360 --> 00:25:43,270
+volume عندي واحد زائد تسعةطبعا هو يساوي واحد على
+
+322
+00:25:43,270 --> 00:25:50,010
+عشرة طبعا واحد على عشرة هذا هو ال .. ال .. ال ..
+
+323
+00:25:50,010 --> 00:25:53,490
+dilution تبع اليوبيا إذا هذه اليوبيا مخففتها عشر
+
+324
+00:25:53,490 --> 00:25:57,870
+مرات من ال .. ال original طبعا تكيزها عشر ال
+
+325
+00:25:57,870 --> 00:26:02,410
+original هي .. هي ال .. ال .. هي البحث بال
+
+326
+00:26:02,410 --> 00:26:11,640
+dilution طيب مثلالو كان عندي انا ال .. ال .. ال ..
+
+327
+00:26:11,640 --> 00:26:14,360
+المسألة هذه يقولي احسبي التركيز هنا، انا كده بدي
+
+328
+00:26:14,360 --> 00:26:19,080
+احسبها، أخدتوا قانون في التحليل يساوي M V يساوي M
+
+329
+00:26:19,080 --> 00:26:25,600
+شارطة V شارطة، هذا ما قبل التخليف، طبعا، و هذا بعد
+
+330
+00:26:25,600 --> 00:26:31,120
+التخليف كان، صح؟و .. او .. و موجود بصوتات نفس
+
+331
+00:26:31,120 --> 00:26:36,540
+القانون ولكن بس احنا معبرين عنه ب C V يساوي C V C1
+
+332
+00:26:36,540 --> 00:26:43,180
+V1 قبل التخييف و C2 V2 بعد التخييف طبعا؟ طيب C1
+
+333
+00:26:43,180 --> 00:26:49,580
+كام؟ كانت 200 بهذا المثال V1 كانت .. لنفرض ان ال B
+
+334
+00:26:49,580 --> 00:26:54,730
+مثلا هي عشر أقل حجمها هناطبعا .. بس هنا مابفيدني
+
+335
+00:26:54,730 --> 00:26:59,570
+.. انا مابفيدني جدش أخد 8 من ال .. منه .. أخد منه
+
+336
+00:26:59,570 --> 00:27:03,530
+واحد عشان أخففه .. يعني ضارب واحد ..ماشي ضارب واحد
+
+337
+00:27:03,530 --> 00:27:09,330
+هنا ..طبعا ال C2 جدش هو المجهول اللي مش معروفة ..
+
+338
+00:27:09,330 --> 00:27:15,060
+ال V2 طبعاال volume هنا إيجى تسعة عشرة في ال tube
+
+339
+00:27:15,060 --> 00:27:20,220
+هنا طبعا واحد تسعة عشرة فهذه المسألة عوّب فيها
+
+340
+00:27:20,220 --> 00:27:27,100
+فهنا مثلا على متين على عشرة هنا على عشرة و هنا على
+
+341
+00:27:27,100 --> 00:27:31,200
+عشرة بيروحوا حضر مع بعض و بيطلع ال concentration
+
+342
+00:27:31,200 --> 00:27:37,670
+C2 عشرينطبعا عشرين إيش؟ عشرين نفس التركيز
+
+343
+00:27:37,670 --> 00:27:42,410
+milligram بالأقل milligram بالأقل طبعا هذا هو
+
+344
+00:27:42,410 --> 00:27:50,470
+تركيز ال .. ال queue إذا لاحظته الأصل ميتين طبعا و
+
+345
+00:27:50,470 --> 00:27:55,110
+المخففة عشرين و المخففة فحكيتلكم إيش معناها عشر
+
+346
+00:27:55,110 --> 00:28:01,910
+الأصل عشر الميتين عشرين طبعافهي هنا ال .. يعني حلو
+
+347
+00:28:01,910 --> 00:28:06,090
+الربط ان طبت المعلومات بعضها البعض طيب كيف تيجي
+
+348
+00:28:06,090 --> 00:28:11,850
+الأسئلة أيضا على هذا الشكل؟ هي هنا مثلا عندي مثال
+
+349
+00:28:11,850 --> 00:28:15,530
+بالملزمة
+
+350
+00:28:15,530 --> 00:28:18,690
+أو بالمحاضرة تبعتنا هي
+
+351
+00:28:23,190 --> 00:28:28,170
+بدي أحضر 45 أمقال من محلول يكون تركيزه 30%
+
+352
+00:28:28,170 --> 00:28:33,150
+solution و ال stock تبعي من الأصل اللي هو 70%
+
+353
+00:28:33,150 --> 00:28:36,610
+تركيزه طبعا هذا كيف أنا بدي أطبقها يعني هلا بدي
+
+354
+00:28:36,610 --> 00:28:42,590
+أطبقهمهي هنا بدي احضر انا تلاتين في المية محلول و
+
+355
+00:28:42,590 --> 00:28:45,670
+ال final volume يكون خمسة و أربعين انا بدي خمسة و
+
+356
+00:28:45,670 --> 00:28:48,750
+أربعين من اقل من المحلول اللي تركيزه تلاتين في
+
+357
+00:28:48,750 --> 00:28:52,630
+المية من original عندي سبعين في المية طب قداش اخد
+
+358
+00:28:52,630 --> 00:28:58,410
+من ال original مش معروف بروح على C1 V1 يساوي C2 V2
+
+359
+00:28:58,410 --> 00:29:04,580
+والله بعد التخفيف تركيزي يكون تلاتينطبعا يا بنات
+
+360
+00:29:04,580 --> 00:29:08,980
+بدنا نوّه انه C1 يعني مقصود بيها concentration و
+
+361
+00:29:08,980 --> 00:29:14,780
+V1 volume one يعني قبل التخفيه ال initial و C2
+
+362
+00:29:14,780 --> 00:29:18,440
+طبعا concentration في volume بعد التخفيف عشان هي
+
+363
+00:29:18,440 --> 00:29:22,080
+اعطيناها الرقم اتنين نفس الفكرة M في V ال M
+
+364
+00:29:22,080 --> 00:29:26,400
+molarity يعني concentration ضرب V volume يساوي ال
+
+365
+00:29:26,400 --> 00:29:30,960
+molarity في ال volume يعني حاطيناهم شرطة يعني بعد
+
+366
+00:29:30,960 --> 00:29:34,440
+التخفيففهذه الفكرة بس عشان نفهم إيش يعني C وإيش
+
+367
+00:29:34,440 --> 00:29:37,460
+يعني V C concentration في volume يساوي ال
+
+368
+00:29:37,460 --> 00:29:41,140
+concentration في volume قبل التخفيف يساوي ما بعد
+
+369
+00:29:41,140 --> 00:29:45,820
+التخفيف هذا القانون أصلا المفروض هو موجود معاكم
+
+370
+00:29:45,820 --> 00:29:49,560
+بالتحليلية اللي هو عبارة عن number of moles يساوي
+
+371
+00:29:49,560 --> 00:29:53,240
+number of moles هذا تاخده في التحليلية مع المعلاقة
+
+372
+00:29:53,240 --> 00:29:56,920
+المهم انتوا تعرفوا ان C1 في V1 C يعني مقصود فيها
+
+373
+00:29:56,920 --> 00:30:02,550
+concentration و V1 مقصود فيها volumeC to
+
+374
+00:30:02,550 --> 00:30:06,130
+concentration و volume و لكن بعد التخفيف نفس فكرة
+
+375
+00:30:06,130 --> 00:30:10,650
+M و V ال M molarity يعني أصلا concentration احنا
+
+376
+00:30:10,650 --> 00:30:15,150
+من شوية لسه شرحنا حكينا ان ال molarity أحد طرق
+
+377
+00:30:15,150 --> 00:30:18,190
+التعبير عن ال concentration ال chemical unit تمام
+
+378
+00:30:18,190 --> 00:30:25,550
+فهي الفكرةو الحجم النهائي بتديها 45 و قبل التخفيف
+
+379
+00:30:25,550 --> 00:30:30,310
+انا عندي 70 فمش معروف عندى ال volume هذا جداش فعلى
+
+380
+00:30:30,310 --> 00:30:36,410
+طول تعويض مباشر تمام فبقسم و بحسب بيطلع معايا
+
+381
+00:30:36,410 --> 00:30:42,750
+الحجم زى ما هو موجود في ال slides 19.3 أمال تمام
+
+382
+00:30:42,750 --> 00:30:48,170
+هذا بيجي هيك طيب ممكن ييجي ايضا ييجي عندى مثلا
+
+383
+00:30:48,170 --> 00:30:54,990
+high etudeأخدنا منها حجم معين تمام بحيث أنه كانت
+
+384
+00:30:54,990 --> 00:30:59,290
+ال .. ال .. هنا تركيز خمسين في المية صار خمسة و
+
+385
+00:30:59,290 --> 00:31:04,890
+عشرين في المية تمام؟ و هنا الحجم أو ال volume
+
+386
+00:31:04,890 --> 00:31:11,370
+النهائي خمس أمقال تمام؟ مش معروف جدش أنا أخدت من
+
+387
+00:31:11,370 --> 00:31:11,630
+هنا
+
+388
+00:31:19,050 --> 00:31:24,010
+طبعا في هذا المثال معروفة ال C1 تركيز الأول هيو 50
+
+389
+00:31:24,010 --> 00:31:29,040
+% ال V1 أنا جدش أخدت هو اللي مجهولC2 خمسة و عشرين
+
+390
+00:31:29,040 --> 00:31:33,880
+في المية معروفة و V2 اللي هو ال final volume او ال
+
+391
+00:31:33,880 --> 00:31:38,560
+volume النهائي هي خمسة أمقال معروفة فيعني اذا V1
+
+392
+00:31:38,560 --> 00:31:41,220
+فقط هو اللي مجهول اللي هو ال volume اللي انا نقلته
+
+393
+00:31:41,220 --> 00:31:44,580
+عشان اعمله dilution تمام هو اللي مش موجود او مش
+
+394
+00:31:44,580 --> 00:31:51,180
+معروف دي التخيل جدش التركيز خمسة و عشرين تمام و
+
+395
+00:31:51,180 --> 00:31:57,790
+جدت او ال volume النهائي خمسة أمقال تماممش معروف
+
+396
+00:31:57,790 --> 00:32:03,950
+جداش انا اخدت من هنا طبعا فعلا طول بروح بحكي C1 V1
+
+397
+00:32:03,950 --> 00:32:09,810
+يساوي C2 V2 بعد التخفيف جداش التركيز خمسة و عشرين
+
+398
+00:32:09,810 --> 00:32:17,950
+طبعا و جداش ال volume النهائي خمسة طيب بس اضبط
+
+399
+00:32:17,950 --> 00:32:24,450
+الأوراق علشان تكون واضحة في ال .. طبعا اذا هي هنا
+
+400
+00:32:32,320 --> 00:32:38,120
+طبعا اذا ال بعد التخفيف كان 25 و ال volume كان 5
+
+401
+00:32:38,120 --> 00:32:41,560
+قبل التخفيف كان 50 بس انا مش عارفة كده اخدت من ال
+
+402
+00:32:41,560 --> 00:32:47,940
+original طبعا على طول بروح اش بعمل تعويض مباشر و
+
+403
+00:32:47,940 --> 00:32:53,840
+بطلع معايا الحجم اعتقد انه هيطلع اتنين و نص امقال
+
+404
+00:32:55,760 --> 00:33:00,720
+طبعا هذا بالنسبة لأ مسائل .. طبعا هذا إيش؟ هذا ال
+
+405
+00:33:00,720 --> 00:33:04,640
+V1 طبعا طلع عندي اتنين و نص
+
+406
+00:33:10,520 --> 00:33:16,580
+طيب هذا بالنسبة لأ ال .. ال .. ال .. الأسئلة طب
+
+407
+00:33:16,580 --> 00:33:19,940
+هانا .. هاي هذا النوع من الأسئلة إيش بحكيلك to ..
+
+408
+00:33:19,940 --> 00:33:23,760
+to make تمانية أمقال أنا بدي أعمل تمانية أمقال
+
+409
+00:33:23,760 --> 00:33:28,640
+يعني ال volume النهائي بدي يكون تمانية أمقالو 1 ل
+
+410
+00:33:28,640 --> 00:33:35,180
+20 dilution يعني انا التركيق بدي اعمل يعني مخفف
+
+411
+00:33:35,180 --> 00:33:41,440
+عشرين مرة يعني الاصل كان تركيزه عشرين و بدي اخففه
+
+412
+00:33:41,440 --> 00:33:44,620
+عشرين مرة عشان اصل له احد هيك معناه كلمة واحد
+
+413
+00:33:44,620 --> 00:33:48,100
+لعشرين خلينا نشوف ايش يعني كيف انا ممكن اطبق
+
+414
+00:33:48,100 --> 00:33:58,380
+باستخدام القانون C1 V1 يساوي C2 V2تمام ال C1 هذا
+
+415
+00:33:58,380 --> 00:34:04,380
+اش كان عندى التركيز الاول كان عشرين مركز عشرين مرة
+
+416
+00:34:04,380 --> 00:34:12,460
+و ال volume هذا مش معروفو ال C2 بدي اخففها مرة
+
+417
+00:34:12,460 --> 00:34:16,040
+واحدة انا بدي اخفف من عشرين لواحد مكتوب لواء one
+
+418
+00:34:16,040 --> 00:34:21,460
+in عشرين يعني من عشرين لواحد بدخليها تمام ال
+
+419
+00:34:21,460 --> 00:34:23,980
+concentration واحد في ال volume جديش ال volume
+
+420
+00:34:23,980 --> 00:34:27,980
+جالي تمانية امقال بدي احضر واحد اتعويض مباشر بجيب
+
+421
+00:34:27,980 --> 00:34:32,320
+بقسم على عشرين على عشرين بجيب هادي ال volume one
+
+422
+00:34:32,520 --> 00:34:37,980
+طبعا هايك بالنسبة لمن ل ال primary dilution كله
+
+423
+00:34:37,980 --> 00:34:43,980
+نفس الفكرة طيب و كيف بجيب ال dilution حكينا ان هو
+
+424
+00:34:43,980 --> 00:34:48,380
+عبارة عن ال ratio او النسبة ما بين ال volume ال
+
+425
+00:34:48,380 --> 00:34:52,500
+concentrated على ال total volume يعني مثلا نرجع
+
+426
+00:34:52,500 --> 00:34:56,600
+نضرب طبعا لما احكي primary dilution او simple
+
+427
+00:34:56,600 --> 00:35:00,660
+dilution نفس المعنى طبعا هو التخفيف اللى يجرى
+
+428
+00:35:00,660 --> 00:35:05,690
+بخطوة واحدةالاخر عشان نأكد الفكرة خلاص ونروح على
+
+429
+00:35:05,690 --> 00:35:09,790
+غيرها مثلا
+
+430
+00:35:09,790 --> 00:35:19,770
+هى في عندى tube تركيزها ثلاثة في المية تمام أخدت
+
+431
+00:35:19,770 --> 00:35:25,210
+منها واحد أو أخدت منها طبعا هى خليني أخدت خمسة
+
+432
+00:35:25,210 --> 00:35:29,570
+أمال في tube وكان في عندى فيها خمسة أمال
+
+433
+00:35:35,590 --> 00:35:41,750
+تمام فجدش ال concentration هنا بدى اروح او sorry
+
+434
+00:35:41,750 --> 00:35:45,150
+بلش قبل اسأل عن ال concentration جدش ال dilution
+
+435
+00:35:45,150 --> 00:35:48,590
+دلوقت يبقى هادى ال dilution قانون ال dilution هو
+
+436
+00:35:48,590 --> 00:35:52,850
+عبارة عن ايش نعمل سلايدات هو عبارة عن ال ratio لل
+
+437
+00:35:52,850 --> 00:35:59,630
+volume of concentrated صح على ال total volumeطيب
+
+438
+00:35:59,630 --> 00:36:04,090
+انا جدش أخدت من ال concentration؟ أخدت خمسة طبعا
+
+439
+00:36:04,090 --> 00:36:08,070
+على ال .. فوق ال total جدش؟ خمسة زائد خمسة عشرة
+
+440
+00:36:08,070 --> 00:36:13,710
+يعني خمسة على عشرة يعني نص طبعا هذا ال dilution نص
+
+441
+00:36:13,710 --> 00:36:16,590
+طب لو جالي احسب ال concentration عطول اش بروح
+
+442
+00:36:16,590 --> 00:36:25,540
+بعمله بحكيله c1 v1 فييساوي C2 V2 C1 جدش كان
+
+443
+00:36:25,540 --> 00:36:30,140
+تركيزها تلاتة في المية تلاتة طبعا ضرب V1 جدش أخدت
+
+444
+00:36:30,140 --> 00:36:34,320
+منها خمسة أمقال ويساوي C2 اللي مش معروفة و ال
+
+445
+00:36:34,320 --> 00:36:39,700
+volume النهائي كان عندي عشرة خمسة و خمسة عشرة طبعا
+
+446
+00:36:39,700 --> 00:36:48,780
+فإذا بعد هيك بطبق طاقة تطبيق مباشر للحسابات
+
+447
+00:36:48,780 --> 00:36:50,280
+و بجيب اللي هو C2
+
+448
+00:36:54,910 --> 00:36:58,090
+طبعا يا بنات انا لما ب .. اكتب القوانين بكتبها
+
+449
+00:36:58,090 --> 00:37:04,950
+مقتصرة لإنه مش مجدي بياخد وقت معايا و انا بحاول ان
+
+450
+00:37:04,950 --> 00:37:08,750
+انا اكتب ال .. القانون كامل ف volume of
+
+451
+00:37:08,750 --> 00:37:11,810
+concentrated اللي هي نفسها volume of sample أو
+
+452
+00:37:11,810 --> 00:37:15,310
+volume of concentrated solution عادي بس انا بختصر
+
+453
+00:37:15,310 --> 00:37:20,350
+في الكتابة ل .. لتوفير الوقت فقط لغبلة و انا بشرح
+
+454
+00:37:20,680 --> 00:37:24,280
+فهذا الاشي يعني خدوه بعين الاعتماد تركيزها تلاتة
+
+455
+00:37:24,280 --> 00:37:29,480
+في المية تلاتة سماء الاحسابات و بجيب اللي هو C2
+
+456
+00:37:29,480 --> 00:37:34,980
+كاتبلي في ال slides انه ال dilution عبارة عن ايه
+
+457
+00:37:34,980 --> 00:37:42,840
+عبارة عن ال volume of sample على ال total volume
+
+458
+00:37:42,840 --> 00:37:46,020
+طب يا miss انت حكيلي ان ال volume of concentrated
+
+459
+00:37:46,020 --> 00:37:49,340
+هنا طب ايش يعني ايش الفرق ما بين
+
+460
+00:37:53,600 --> 00:37:58,200
+طبعا الفرق ما بينهم طبعا مافيش فرق سواء انا حكيت
+
+461
+00:37:58,200 --> 00:38:01,480
+سواء كان عندي stock solution او concentrated
+
+462
+00:38:01,480 --> 00:38:05,140
+solution بدي اعمله تخفيف او سواء كان عندي sample
+
+463
+00:38:05,140 --> 00:38:10,070
+عين انا بدي اخففها و اشتغل عليها نفس المعنىيعني فى
+
+464
+00:38:10,070 --> 00:38:13,350
+النهاية اللى بيكون فى البسط هو ال volume اللى
+
+465
+00:38:13,350 --> 00:38:16,850
+أخدته من المادة المركزة أو المادة المراد تخفيفها
+
+466
+00:38:16,850 --> 00:38:20,850
+سواء كانت محلول أو عينة وعلى ال final volume جدش
+
+467
+00:38:20,850 --> 00:38:25,920
+صار فى الحجم النهائى بعد ما ضفت ال solvent عليهاهي
+
+468
+00:38:25,920 --> 00:38:29,860
+الفكرة او هيها ده معنى قانون ال delution تمام و
+
+469
+00:38:29,860 --> 00:38:34,160
+احنا ذكرنا سابقا في المحاضرة المسجلة انه انا
+
+470
+00:38:34,160 --> 00:38:38,200
+احيانا بضطر اخفف عينات لإنه التركيزها بيكون عالي و
+
+471
+00:38:38,200 --> 00:38:40,920
+الأجهزة اللي عندي و المواد اللي عندي قدرة
+
+472
+00:38:40,920 --> 00:38:43,880
+استيعابها مابتقدرش تجيس للتركيز اللي موجودة في
+
+473
+00:38:43,880 --> 00:38:47,460
+العينة وضربتلكم مثال لما يكون السكر تركيزه 500
+
+474
+00:38:47,460 --> 00:38:54,500
+وعندي محلول بيجيس يعنيلأ انكترت يعني ان لأ يعني
+
+475
+00:38:54,500 --> 00:38:57,420
+على أقصى استيعاب عنده بي .. بيجيسل لحد تلاتمية
+
+476
+00:38:57,420 --> 00:39:00,960
+فإيش أعمل أنا بهاي الحالة؟ بخفف العينة طب كيف بدي
+
+477
+00:39:00,960 --> 00:39:04,800
+أخفف العينة؟ بدي أستخ .. أاخد منها حجم و أضيفه على
+
+478
+00:39:04,800 --> 00:39:08,300
+ال sample فأضيف حجم من ال sample و أضيف عليه
+
+479
+00:39:08,300 --> 00:39:11,410
+solventطبعا كيف بدى أحسب ال dilution للتخفيف
+
+480
+00:39:11,410 --> 00:39:14,830
+التخفيف اللى حصل هنا للعينة جدش أنا أخدت من ال
+
+481
+00:39:14,830 --> 00:39:18,750
+sample على حجم ال sample مع ال solvent كلهم سوا ال
+
+482
+00:39:18,750 --> 00:39:23,150
+total هيك بكون طلعت ال dilution تمام الجزء الآخر
+
+483
+00:39:23,150 --> 00:39:30,090
+من المحاضرة لحظة هى الجزء الآخر انا طبعا مصوراهم ف
+
+484
+00:39:30,090 --> 00:39:36,530
+..هى اللى احنا بنكون مركزينها يعنى تم فصلهم لجزئين
+
+485
+00:39:36,530 --> 00:39:39,450
+يعنى ما بعرف ليش انفصلوا او فصل التسجيل اثناء
+
+486
+00:39:39,450 --> 00:39:43,710
+تصويرى ما علينا هذا هو الجزء الاخر نستكمل من عند
+
+487
+00:39:43,710 --> 00:39:49,310
+القانون ال delution القانون التانى لش كان تتملناه
+
+488
+00:39:49,310 --> 00:39:52,230
+فى ال slides ان هو volume of sample على ال total
+
+489
+00:39:52,230 --> 00:39:55,450
+volume هى هنا شرح و إعادة لنفس الكلام اللى ذكرته
+
+490
+00:39:55,450 --> 00:40:00,690
+من شويةهي اللي احنا بنكون مركزين بتكون بدأ أخففها
+
+491
+00:40:00,690 --> 00:40:03,870
+أو بتكون مركزة هي نفس الفكرة يعني volume of sample
+
+492
+00:40:03,870 --> 00:40:06,950
+او volume concentrated نفس الاشياء على ال total
+
+493
+00:40:06,950 --> 00:40:11,210
+volumeطيب لو انا بدي اجيب هذه ال cube ال dilution
+
+494
+00:40:11,210 --> 00:40:15,170
+تبعها نص لو بدي اجيب حالة اسمها dilution factor
+
+495
+00:40:15,170 --> 00:40:18,650
+ايش يعني يعني مقلوب النص مقلوب ال dilution ال
+
+496
+00:40:18,650 --> 00:40:22,830
+dilution factor هو عبارة عن واحد على ال dilution و
+
+497
+00:40:22,830 --> 00:40:33,130
+يساوي واحد على ال واحد على النص يعني اتنين مقلوب
+
+498
+00:40:33,130 --> 00:40:37,700
+ال dilution يعني اتنينتمام هذا هو ال dilution
+
+499
+00:40:37,700 --> 00:40:40,480
+factor بنزمني كمان شوية لما احكي عن ال cereal
+
+500
+00:40:40,480 --> 00:40:49,940
+dilution طيب نيجي نحكي عن ال cereal dilutions كيف
+
+501
+00:40:49,940 --> 00:40:54,020
+انا بستخدم .. ب .. بعمل ال cereal dilution تمام ال
+
+502
+00:40:54,020 --> 00:40:56,680
+cereal dilution حاكية انه مثلا لو كان عندى تيوب
+
+503
+00:40:56,680 --> 00:41:05,440
+تركيزين تمام molar ومحلول مثلا ال glucoseطمام؟ و
+
+504
+00:41:05,440 --> 00:41:10,920
+أنا أخدت منه one ml و حطيتها في تيوب فيها تسعة ml
+
+505
+00:41:10,920 --> 00:41:14,720
+distilled water بعدين أخدت في تيوب تانية one ml و
+
+506
+00:41:14,720 --> 00:41:18,240
+حطيتها في تيوب فيها تسعة ml من ال distilled water
+
+507
+00:41:18,240 --> 00:41:21,960
+و أخدت في .. في تيوب خمسة ml و حطيتها في تيوب كان
+
+508
+00:41:21,960 --> 00:41:26,190
+فيها خمسة ml من ال distilled waterانا عندها الـ
+
+509
+00:41:26,190 --> 00:41:29,790
+get a tube رقم واحد اتنين تلاتة تلت قنابيب انا
+
+510
+00:41:29,790 --> 00:41:33,570
+عاملة لهم تخطيط طيب لو انا بدي اجي اعمل حسابات
+
+511
+00:41:33,570 --> 00:41:37,510
+لهاي التلت قنابيب اول حاجة بدي احسب ال dilution
+
+512
+00:41:37,510 --> 00:41:41,030
+لتلت قنابيب هو عبارة عن ال volume ل ال
+
+513
+00:41:41,030 --> 00:41:46,430
+concentrated على ال total volume صح؟ طيب ال .. كده
+
+514
+00:41:46,430 --> 00:41:50,490
+اش انا خدت من ال total واحد هي واحد على ال total
+
+515
+00:41:50,490 --> 00:41:56,430
+واحد زائد تسعة يساوي عشر عشرطيب ال tube هذه انا
+
+516
+00:41:56,430 --> 00:41:59,350
+جدش اخدت من ال .. من اللي جا .. من ال concentrated
+
+517
+00:41:59,350 --> 00:42:02,830
+يعني من اللي جابلها المركزة و اكتر منها اخدت واحد
+
+518
+00:42:02,830 --> 00:42:08,810
+طبعا على واحد زائد تسعة عشرة بنفع اقول ال tube هذه
+
+519
+00:42:08,810 --> 00:42:15,000
+تخفيفها عشر و سقط لأهذه تخفيفها عشر من الأصل لكن
+
+520
+00:42:15,000 --> 00:42:18,320
+هذه تخفيفها عشر من ال .. من التيوبة اللي جابلها
+
+521
+00:42:18,320 --> 00:42:23,420
+اللي ألضم تخفيفها عشر يعني ان التيوبة رقم واحد صح
+
+522
+00:42:23,420 --> 00:42:27,520
+تخفيفها طولها عشر، واحد على عشرة بس هي من الأصل
+
+523
+00:42:27,520 --> 00:42:32,430
+عشرفواحدة .. لو أنا جيت حكيت dilution of تيوب one
+
+524
+00:42:32,430 --> 00:42:37,490
+واحد على عشرة و ساكتت، عادي، تمام؟ فلإنه .. لإنها
+
+525
+00:42:37,490 --> 00:42:41,430
+جاية من الأصل لكن هاي ما بنفع أحكي إن هاي التيوب
+
+526
+00:42:41,430 --> 00:42:45,570
+تخفيفها أيضا واحد لعشرة، لأ هي تخفيفها واحد لعشرة
+
+527
+00:42:45,570 --> 00:42:50,160
+من التيوب اللي سابقاهاتمام؟ فما بنفع أحكي انه
+
+528
+00:42:50,160 --> 00:42:53,620
+تخفيفها واحد لعشرة واسكت لأ بدي أكمل بدي أحكي واحد
+
+529
+00:42:53,620 --> 00:42:57,980
+لعشرة من مين؟ من التيوب اللي سبقتها اللي أصلا أيضا
+
+530
+00:42:57,980 --> 00:43:02,000
+تخفيفها واحد لعشرة فأصبح بيصير تخفيف التيوب رقم
+
+531
+00:43:02,000 --> 00:43:05,620
+اتنين واحد لعشرة من التيوب رقم واحد اللي تخفيفها
+
+532
+00:43:05,620 --> 00:43:09,500
+ايضا واحد لعشرة طب كيف ده اختصر هذا الكلام كله؟
+
+533
+00:43:09,500 --> 00:43:13,660
+بدي اقول واحد لعشرة درب واحد لعشرة اللي هنا ويسوي
+
+534
+00:43:13,660 --> 00:43:19,400
+واحد على مية تخفيفهاهي معاكم الشريح حلو بتشوفوا
+
+535
+00:43:19,400 --> 00:43:22,080
+كده، اذا انا لو بدي اجيب ال dilution اللي هي ده
+
+536
+00:43:22,080 --> 00:43:27,740
+المفروض اقول عشر اللي الها جاي من العشر اللي
+
+537
+00:43:27,740 --> 00:43:33,190
+جابلها يعني عشر في عشر و يسوي واحد عالميامضربهم في
+
+538
+00:43:33,190 --> 00:43:38,730
+بعض طيب نفس الفكرة هنا جدش ال delution أخدت خمسة
+
+539
+00:43:38,730 --> 00:43:42,550
+من المركز اللي جابلها أو الأكتر تركيزا منها خمسة
+
+540
+00:43:42,550 --> 00:43:46,950
+على إيش خمسة زائد خمسة عشرة يعني إيش نص مابنفع
+
+541
+00:43:46,950 --> 00:43:53,390
+أقول نص و أسكت لأ نص من المخففة نص من اللي جابلها
+
+542
+00:43:54,070 --> 00:43:59,210
+طبعا لو أنا قلت نص و أسكت معناها أنها مخففة نص من
+
+543
+00:43:59,210 --> 00:44:02,950
+ال original و هذا غلط لأ هي مخففة نص من اللي قبلها
+
+544
+00:44:02,950 --> 00:44:07,610
+يعني نص ضرب اللي قبلها جداش واحدة عالمية يسوى واحد
+
+545
+00:44:07,610 --> 00:44:08,630
+على تان
+
+546
+00:44:10,980 --> 00:44:13,920
+ال .. ال .. ال dilution اذا هذه تخطيطها واحد لعشرة
+
+547
+00:44:13,920 --> 00:44:17,700
+تيوب رقم واحد تيوب رقم اتنين واحد لمية تيوب رقم
+
+548
+00:44:17,700 --> 00:44:22,600
+تلاتة واحد لمتان طيب لو انا بدى اجيب التركيز انا
+
+549
+00:44:22,600 --> 00:44:26,220
+.. انا عارفة ان ال original تركيزها مية طيب جداش
+
+550
+00:44:26,220 --> 00:44:28,880
+بدى اجيب .. لو بدى اجيب التركيز عفوا ان ال
+
+551
+00:44:28,880 --> 00:44:31,840
+original تركيزها متين هلأ انا بدى اجيب تركيز
+
+552
+00:44:31,840 --> 00:44:36,900
+الانبيب كيف بدى اشتغل التركيز لكل تيوب اش بدى اعمل
+
+553
+00:44:36,900 --> 00:44:43,370
+التركيز ل .. of تيوبconcentration of a tube تركيز
+
+554
+00:44:43,370 --> 00:44:50,210
+الأنابيب اللى عندى تساوي ال dilution of a tube
+
+555
+00:44:50,210 --> 00:44:56,390
+تمام ضرب ال original concentration
+
+556
+00:44:58,990 --> 00:45:02,870
+طيب اش يعني هاي ال tube رقم واحد؟ ال tube رقم واحد
+
+557
+00:45:02,870 --> 00:45:05,510
+ال dilution of ال tube يعني ال dilution of ال tube
+
+558
+00:45:05,510 --> 00:45:11,350
+رقم واحد جداش بيطلع واحد على عشرة طبعا ضرب ال
+
+559
+00:45:11,350 --> 00:45:14,190
+original concentration جداش ال original
+
+560
+00:45:14,190 --> 00:45:18,150
+concentration 200 إذا بضرب في 200 و بده يطلع ليه
+
+561
+00:45:18,150 --> 00:45:27,460
+عشرين molar طيب هاي تخفيفهاواحر عالمية، إذا الـ
+
+562
+00:45:27,460 --> 00:45:31,660
+dilution تبعها واحر عالمية ضارب 200 ويساوي 2 molar
+
+563
+00:45:31,660 --> 00:45:38,140
+طب هالي تخفيكها واحر على 200 في 200 تركيز ال
+
+564
+00:45:38,140 --> 00:45:43,220
+original concentration ويساوي 1 molar إذا أنا بقدر
+
+565
+00:45:43,220 --> 00:45:46,440
+من خلال هذا القانون ال concentration of a tube
+
+566
+00:45:46,440 --> 00:45:49,440
+أجيب تركيز القنابيب التلاتة اللي حصلت عليهم في ال
+
+567
+00:45:49,440 --> 00:45:55,560
+cereal dilutionطيب على فرض .. على فرض أنه هذه
+
+568
+00:45:55,560 --> 00:45:59,920
+القيم .. هذه القيم أعطانيها .. هو أعطانيها
+
+569
+00:46:03,010 --> 00:46:09,390
+ولكن ولكن ان هو انه ال original اللى كان مجهول مش
+
+570
+00:46:09,390 --> 00:46:12,190
+معروف انا مش عارفة ال original هذا جداش بدى
+
+571
+00:46:12,190 --> 00:46:16,090
+بالاكاد بدى احسبه خلينى نشوف ما هو يا اما بيعطينى
+
+572
+00:46:16,090 --> 00:46:19,010
+ال original و بقولى احسب ال tube ال concentration
+
+573
+00:46:19,010 --> 00:46:21,830
+of ال tube يا اما بيعطينى ال concentration of ال
+
+574
+00:46:21,830 --> 00:46:25,110
+tube و بقولى احسب ال original هلأ انا افترض انه
+
+575
+00:46:25,110 --> 00:46:28,610
+معاكي ال concentration of ال tube و بدى تحسبيه ال
+
+576
+00:46:28,610 --> 00:46:34,750
+original هى ال originalconcentration يساوي ال
+
+577
+00:46:34,750 --> 00:46:42,170
+dilution factor of ال cube ضرب ضرب تمام ال
+
+578
+00:46:42,170 --> 00:46:49,970
+concentration of the same cube اش يعني خليني نطبق
+
+579
+00:46:49,970 --> 00:46:53,050
+ال cube رقم واحد ال cube رقم واحد قداش ال dilution
+
+580
+00:46:53,050 --> 00:46:56,700
+factorال dilution factor احنا حكينا مطلوب ال
+
+581
+00:46:56,700 --> 00:47:01,020
+dilution يعني هنا ال dilution واحد على عشرة معناها
+
+582
+00:47:01,020 --> 00:47:05,980
+ال dilution factor يساوي عشرة تمام؟ يساوي عشرة هنا
+
+583
+00:47:05,980 --> 00:47:12,840
+ال dilution factor فإذا ال dilution factor عشرةالـ
+
+584
+00:47:12,840 --> 00:47:15,620
+Concentration of the same tube قدش ميعطينياها
+
+585
+00:47:15,620 --> 00:47:19,600
+نفترض انه عطانياها عشرين عشرة في عشرين ويساوي متين
+
+586
+00:47:19,600 --> 00:47:25,040
+تمام حساباتنا صح طيب خلينا نطبق في ال tube رقم
+
+587
+00:47:25,040 --> 00:47:28,580
+اتنين ال tube رقم اتنين هنا ال dilution factor
+
+588
+00:47:28,580 --> 00:47:32,640
+مقلوب ال dilution قداش مية واحدة عالمية مقلوبها
+
+589
+00:47:32,640 --> 00:47:37,480
+مية مية في اتنين200 الـ dilution factor لها دي 200
+
+590
+00:47:37,480 --> 00:47:42,780
+مقلوبها واحد على 200 طبعا هي ال dilution ال
+
+591
+00:47:42,780 --> 00:47:46,520
+dilution factor 200 200 في واحد 200 اذا ال
+
+592
+00:47:46,520 --> 00:47:49,880
+original بقدر احسبه ان هو عبارة عن ال dilution
+
+593
+00:47:49,880 --> 00:47:53,340
+factor لليتيوب ضرب التركيز نفس ليتيوب مابنفع اقول
+
+594
+00:47:53,340 --> 00:47:56,000
+والله ال dilution factor لها دي ليتيوب عشرة وروح
+
+595
+00:47:56,000 --> 00:48:00,350
+اضربه في تركيز ليتيوب رقم اتنين غلططبعا لازم انه
+
+596
+00:48:00,350 --> 00:48:04,890
+اضربه في تركيز نفس ال cube اللي هو عشرة في عشرين
+
+597
+00:48:04,890 --> 00:48:12,070
+مية في اتنين متين في واحد و هكذا طبعا طيب نضرب
+
+598
+00:48:12,070 --> 00:48:18,310
+منها الاخر و ننهي فيه علشان يكون معنى واضح كل إشي
+
+599
+00:48:18,310 --> 00:48:22,190
+أخدت عن ال cube طبعا .. طبعا هذه ال stock او قولنا
+
+600
+00:48:22,190 --> 00:48:30,360
+ال original طبعاتركيزها مثلا خمسمية ميلي اجرام
+
+601
+00:48:30,360 --> 00:48:36,380
+بالديسيلاتر أخدت منها اش حاكينا واحد امقلتمام و
+
+602
+00:48:36,380 --> 00:48:40,240
+عندي تسعة أمقال distilled water و أخدت كمان أيضا
+
+603
+00:48:40,240 --> 00:48:45,080
+واحد أمقال و عندي تسعة أمقال distilled water تمام
+
+604
+00:48:45,080 --> 00:48:49,760
+و .. او خليني أحكي مثلا هنا أخدت اتنين أمقال و في
+
+605
+00:48:49,760 --> 00:48:54,200
+عندي تمانية أمقال distilled water و هنا مثلا أخدت
+
+606
+00:48:54,200 --> 00:49:00,940
+خمسة أمقال و في عندي خمسة أمقال distilled water
+
+607
+00:49:01,360 --> 00:49:05,480
+طبعا يلّا نحسب ال dilution للقنابيب التلاتة هي
+
+608
+00:49:05,480 --> 00:49:09,640
+عندي انبوبة رقم واحد انبوبة رقم اتنين انبوبة رقم
+
+609
+00:49:09,640 --> 00:49:14,380
+تلاتة ال dilution قولنا ال volume of concentrated
+
+610
+00:49:14,380 --> 00:49:18,300
+على ال total volume ال concentrated على ال total
+
+611
+00:49:18,300 --> 00:49:24,080
+volume طبعا واحد لل tube رقم واحد واحد على واحد
+
+612
+00:49:24,080 --> 00:49:28,820
+زائد تسعة يعني واحد على عشرةاتنين على اتنين زائد
+
+613
+00:49:28,820 --> 00:49:33,460
+تمانية اتنين على اتنين زائد تمانية ويساوي اتنين
+
+614
+00:49:33,460 --> 00:49:37,800
+على عشرة قولنا بنسكت هنا لأ اتنين على عشرة من مين
+
+615
+00:49:37,800 --> 00:49:43,420
+من اللي جابلها تمام فبتصير اتنين على عشرة ضرب واحد
+
+616
+00:49:43,420 --> 00:49:49,620
+على عشرة ويساوي اتنين على مية تمام يعني مثلا واحد
+
+617
+00:49:49,620 --> 00:49:56,250
+على خمسين ال daily oceanطيب خلينا نشوف ال tube رقم
+
+618
+00:49:56,250 --> 00:50:00,410
+تلاتة جديش كان ال ball اللي أخدناها من اللي جابلها
+
+619
+00:50:00,410 --> 00:50:06,450
+خمسة تمام على خمسة زائد خمسة ال total عشرة يعني نص
+
+620
+00:50:06,450 --> 00:50:11,870
+نص واسكت لأ نص في اللي .. نص من اللي جابلها يعني
+
+621
+00:50:11,870 --> 00:50:19,520
+نص ضرب واحد على خمسين ويساوي واحد على مية تمامهذا
+
+622
+00:50:19,520 --> 00:50:23,260
+اللي هي dilution طيب ال dilution factor خلينا
+
+623
+00:50:23,260 --> 00:50:28,440
+نحسبه بالمرة هذه عشرة هذه خمسين هذه مية المقلوب
+
+624
+00:50:28,440 --> 00:50:32,000
+طبعا اللي هو عبارة عن إيش واحدة على ال dilution
+
+625
+00:50:32,000 --> 00:50:36,880
+طبعا طيب
+
+626
+00:50:36,880 --> 00:50:43,540
+ماشي إذا هحسبنا ال dilution طب ال concentration of
+
+627
+00:50:43,540 --> 00:50:49,700
+tubesتمام ال concentration of tubes اللي هو حكينا
+
+628
+00:50:49,700 --> 00:50:54,340
+عبارة عن ال dilution ضرب ال original واحدة على
+
+629
+00:50:54,340 --> 00:51:00,560
+عشرة في خمسمية تمام خمسين واحدة على خمسين في
+
+630
+00:51:00,560 --> 00:51:10,790
+خمسمية عشرة تمام واحدة على مية في خمسمية خمسةهي
+
+631
+00:51:10,790 --> 00:51:15,450
+التركيز طبعا اش نفس الوحدة بياخده milligram per
+
+632
+00:51:15,450 --> 00:51:20,810
+deciliter طبعا نفس وحدة الاصل طيب على فرض حكينا
+
+633
+00:51:20,810 --> 00:51:26,010
+انه مثلا نفترض جدلا انه هذه ماعطانيها طبعا
+
+634
+00:51:26,010 --> 00:51:29,770
+ماعطانيها و ماعطيني هاي التركيز هادي بدي احسب ال
+
+635
+00:51:29,770 --> 00:51:36,490
+concentration ال concentration oforiginal اش بدو
+
+636
+00:51:36,490 --> 00:51:41,390
+يكون؟ بدو يكون ال dilution factor في ال .. ال ..
+
+637
+00:51:41,390 --> 00:51:45,010
+ال concentration للي تيوب صح؟ ال concentration للي
+
+638
+00:51:45,010 --> 00:51:51,410
+تيوب طيب يعني عشرة في خمسين هاي ال dilution factor
+
+639
+00:51:51,410 --> 00:51:53,710
+و هاي ال concentration للي تيوب عشرة في خمسين
+
+640
+00:51:53,710 --> 00:51:58,570
+خمسمية اذا شغل صح خمسة في عشر .. خمسين في عشرة
+
+641
+00:51:58,570 --> 00:52:04,520
+خمسمية مية في خمسةخمسمية تمام ممكن هذه ما اعطيكي
+
+642
+00:52:04,520 --> 00:52:07,360
+اياها انا و احكيلك .. و اعطيكي تركيز واحدة من
+
+643
+00:52:07,360 --> 00:52:12,620
+القنابيب التانية و اقولك احسبي يالا ممكن مثلا اكون
+
+644
+00:52:12,620 --> 00:52:18,120
+اعطياكي تركيز تيوبة واحدة و اقولك عادي كملي احسب
+
+645
+00:52:18,120 --> 00:52:21,960
+تركيز القنابيب التانية و احسب الاصل سهل جدا بقراص
+
+646
+00:52:21,960 --> 00:52:26,300
+لو انك انت تتبعي القوانين مهم جدا تعرفي ايش قانون
+
+647
+00:52:26,300 --> 00:52:30,090
+ال delusionأيش قانون ال dilution factor؟ أيش قانون
+
+648
+00:52:30,090 --> 00:52:33,110
+ال concentration of the tube؟ اللي احنا كتبناه
+
+649
+00:52:33,110 --> 00:52:37,070
+وقولنا عبارة عن ال dilution في ال original
+
+650
+00:52:37,070 --> 00:52:40,890
+concentration تمام؟ وقانون ال original
+
+651
+00:52:40,890 --> 00:52:43,290
+concentration اللي هو عبارة عن ال dilution factor
+
+652
+00:52:43,290 --> 00:52:47,340
+of الدرب ال concentration of the semi tubeهدول مهم
+
+653
+00:52:47,340 --> 00:52:52,100
+جدا انو تعرفوها حاولوا انو اتدربوا ايداكوا على ال
+
+654
+00:52:52,100 --> 00:52:55,600
+.. الأسئلة اللي موجودة في الملزمة في نفس ال
+
+655
+00:52:55,600 --> 00:52:59,000
+chapter علشان انو تشوفوا حالكم كيف انتوا بقى مع
+
+656
+00:52:59,000 --> 00:53:04,380
+الشرح هل استفدتوا او لأ و انا ممكن ابقى قاعد هيك
+
+657
+00:53:04,380 --> 00:53:08,020
+برضه ابعتلكم أسئلة و احكيلكم جربوا تحلوها تمام
+
+658
+00:53:08,020 --> 00:53:12,780
+فسهل جدا بس تطبيق قوانين و حسابات يعني بس اطمشتي
+
+659
+00:53:12,780 --> 00:53:20,330
+ال .. ذاكرتك الحسابيةبالنسبة للحسابات
+
+660
+00:53:20,330 --> 00:53:24,450
+و التخفيفات طبعا هذا ايش نحكي عنه serial dilution
+
+661
+00:53:24,450 --> 00:53:27,490
+لإنه أنا بمشي بشري .. بشري طريقة بعمل multiple
+
+662
+00:53:27,490 --> 00:53:32,050
+dilution و بمشي بطريقة متسلسلة و ال .. ال .. ال ..
+
+663
+00:53:32,050 --> 00:53:35,190
+التخفيك هذا بيكون من الأصل و هذه .. و التخفيف تبع
+
+664
+00:53:35,190 --> 00:53:37,590
+الكيوب التانية بيكون من الأولى و التارتة من
+
+665
+00:53:37,590 --> 00:53:42,520
+التانية و هكذاعشان هيك بسميه serial متسلسل تمام
+
+666
+00:53:42,520 --> 00:53:47,740
+نيجي لسلايدات اخر slide مثلا هي هنا بيعطيني مثال
+
+667
+00:53:47,740 --> 00:53:48,900
+هنا
+
+668
+00:53:59,300 --> 00:54:07,700
+عينة بكتيريا اخد منها واحد امقال وحطها على تسعة
+
+669
+00:54:07,700 --> 00:54:10,860
+امقال يعني ال total صار عشرة يعني ال dilution اللى
+
+670
+00:54:10,860 --> 00:54:15,560
+هدى اش واحد على عشرة تمام طيب اخد منها واحد امقال
+
+671
+00:54:15,560 --> 00:54:18,640
+وكان في عندى تسعة امقال distilled water يعني برضه
+
+672
+00:54:18,640 --> 00:54:22,540
+واحد على واحد زائد تسعة يعني واحد على عشرة من مين؟
+
+673
+00:54:22,760 --> 00:54:27,300
+من هذه يعني واحد على عشر ضرب واحد على عشر يعني
+
+674
+00:54:27,300 --> 00:54:31,420
+واحد على مية تمام طيب أخدنا من الواحد على مية هذه
+
+675
+00:54:31,420 --> 00:54:35,880
+one ml وحطيتها على تسعة ml distilled water فصار
+
+676
+00:54:35,880 --> 00:54:39,660
+عندى تخفيفها واحد على عشرة من مين من الواحد على
+
+677
+00:54:39,660 --> 00:54:43,280
+مية يعني واحد على عشر ضرب واحد على مية يعني واحد
+
+678
+00:54:43,280 --> 00:54:48,280
+على الف اذا التخفيف ال dilution اشماله واحد على
+
+679
+00:54:48,280 --> 00:54:52,230
+الف يعني عشر أسالت تلاتةو ال dilution factor ألف
+
+680
+00:54:52,230 --> 00:54:56,690
+طبعا هنا في نقطة مهمة لازم احنا نوح لها انه لما
+
+681
+00:54:56,690 --> 00:55:02,010
+اجي احكيلك احسري ال final dilution ايش يعني final
+
+682
+00:55:02,010 --> 00:55:06,330
+dilution مكتوب بالسلايدات انه ال final dilution هو
+
+683
+00:55:06,330 --> 00:55:13,310
+حاصل ضرب تخفيفاتهم التلاتة او هو تخفيف البوبة
+
+684
+00:55:13,310 --> 00:55:19,800
+الأخيرة يعني مثلا هذه كانت واحد على تسعة عشرو هاد
+
+685
+00:55:19,800 --> 00:55:25,560
+اتنين على تمانية اتنقل هي كانت يعني اتنين على عشرة
+
+686
+00:55:25,560 --> 00:55:31,140
+يعني خمس و هاد كانت نص تمام لو جيت ضربت من نص
+
+687
+00:55:31,140 --> 00:55:34,960
+بالاتنين على عشرة بالواحد على عشرة بتطلع عندى اللى
+
+688
+00:55:34,960 --> 00:55:38,040
+هو تركيز التخليف الانبوبة الأخيرة اللى هو واحدة
+
+689
+00:55:38,040 --> 00:55:41,440
+على ميةطبعا نفس الشغل اللى كنا نشتغله مش انا كنت
+
+690
+00:55:41,440 --> 00:55:44,460
+هانا طلعتها واحد على عشرة بعدين هذه قلت اتنين على
+
+691
+00:55:44,460 --> 00:55:50,020
+عشرة يعني خمس صح هذه خمسة خمس طبعا خمس في الواحد
+
+692
+00:55:50,020 --> 00:55:55,320
+على عشرة و هانا طلعت انه ال ..وطلع عندى واحدة على
+
+693
+00:55:55,320 --> 00:55:59,240
+خمسين وانا طلعتها انها نص وضربتها بالواحدة على
+
+694
+00:55:59,240 --> 00:56:02,840
+خمسين يعني سواء طلعت وقولت هذه عشر وهذه اتنين على
+
+695
+00:56:02,840 --> 00:56:06,620
+عشرة وهذه نص وضربتهم في بعض او اشتغلت الشغل هذا
+
+696
+00:56:06,620 --> 00:56:12,020
+لإنه انا طلع كل dilution لحال نفس الاشي المهم انه
+
+697
+00:56:12,020 --> 00:56:15,800
+انا ال final dilution هو تخفيك للانبوبة الأخيرة او
+
+698
+00:56:15,800 --> 00:56:19,920
+حاصل ده التخفيك اللى انا بيدل حالها تمام هذا
+
+699
+00:56:19,920 --> 00:56:26,880
+بالنسبة لالـ Final Dilution لما أجي أطلب أحكي الـ
+
+700
+00:56:26,880 --> 00:56:29,660
+Final Dilution عادي هي بتعطيني تخفيق الأنبوبة
+
+701
+00:56:29,660 --> 00:56:34,060
+الأخيرة يا إما بتقولي هذه واحدة على عشرة وسكتنا
+
+702
+00:56:34,060 --> 00:56:39,440
+وهذه اتنين على عشرة وسكتنا وهذه نص وسكتنا وبعدين
+
+703
+00:56:39,440 --> 00:56:44,520
+ضربناهم في بعضطبعا او بنقول واحد على عشرة و بعدين
+
+704
+00:56:44,520 --> 00:56:47,720
+بنقول هذه اتنين على عشرة و مانسكتش و مانضربها في
+
+705
+00:56:47,720 --> 00:56:52,100
+الواحد على عشرة اللي قبلها بعدين نقول ان هذه نص و
+
+706
+00:56:52,100 --> 00:56:54,660
+بعدين مانسكتش و مضربها في الواحد على خمسين اللي
+
+707
+00:56:54,660 --> 00:56:58,660
+قبلها و تطلع لي واحد على مية و اقول ان هذا هو ال
+
+708
+00:56:58,660 --> 00:57:02,280
+final conclusion سواء اشتغلت هيك او هيك نفس الفكرة
+
+709
+00:57:02,280 --> 00:57:11,650
+طبعا اذاهيك بنكون خلصنا المحاضرة تبع ال solution
+
+710
+00:57:11,650 --> 00:57:14,730
+and dilution و أتمنى أنه يكون الاشي هذا ساعدكم
+
+711
+00:57:14,730 --> 00:57:19,570
+بالفهم و طريقة الشرح على الورق قربت الاشي لأذهانكم
+
+712
+00:57:19,570 --> 00:57:25,170
+و خلت الوضع يعني يمكن تخيله أو يمكن إدراكه و
+
+713
+00:57:25,170 --> 00:57:26,030
+يعطيكم العافية
+
+714
+00:57:30,330 --> 00:57:34,410
+طيب يا صبايا هلأ المطلوب منكم أنه المسائل اللي
+
+715
+00:57:34,410 --> 00:57:39,330
+موجودة بالمحاضرة تمام ترجعوا تحلوها و تشوفوا
+
+716
+00:57:39,330 --> 00:57:44,470
+تستفيدوا من الأفكار اللي موجودة فيها تمام و تشوفي
+
+717
+00:57:44,470 --> 00:57:47,790
+حالك أنت هلأ فاهمة أو لأ اتعاملي مع السؤال زي كأنه
+
+718
+00:57:47,790 --> 00:57:50,970
+الإجابة مش موجودة في الملزمة أو في ال .. في ال
+
+719
+00:57:50,970 --> 00:57:54,170
+power point و حللي لحالك قايمي نفسك شوفي حالك
+
+720
+00:57:54,170 --> 00:57:59,200
+فاهمة أو لأ علشانمحاضرة المناقشة على ال google
+
+721
+00:57:59,200 --> 00:58:02,960
+meet يوم السبت يكون فيها انتوا تناقشوني بالاشياء
+
+722
+00:58:02,960 --> 00:58:05,420
+اللي انتوا مش فاهمينها او اللي مش قادرين تفهموها
+
+723
+00:58:05,420 --> 00:58:09,640
+طبعا عشان اقدر اجاوبكم عليها و نعالج الاشي اول
+
+724
+00:58:09,640 --> 00:58:13,920
+بأول الدراسة اول بأول و المراجعة و انك انت تثبت
+
+725
+00:58:13,920 --> 00:58:18,040
+المعلومات هذا بفيدك اكتر و ان شاء الله يعني هيك ما
+
+726
+00:58:18,040 --> 00:58:22,420
+بيكون اشي عليكم صعب يعطيكم الف عافية و مفقين بإذن
+
+727
+00:58:22,420 --> 00:58:22,640
+الله
+
diff --git a/PL9fwy3NUQKwY9jl8xw-bWkVZkWcGGyspd/-PkMjabV1O4_raw.json b/PL9fwy3NUQKwY9jl8xw-bWkVZkWcGGyspd/-PkMjabV1O4_raw.json
new file mode 100644
index 0000000000000000000000000000000000000000..1e0928206cc52afa80120441fb92960b78d87563
--- /dev/null
+++ b/PL9fwy3NUQKwY9jl8xw-bWkVZkWcGGyspd/-PkMjabV1O4_raw.json
@@ -0,0 +1 @@
+{"segments": [{"id": 1, "seek": 3041, "start": 3.49, "end": 30.41, "text": "مرحبا صبايا هلأ احنا لما شرحنا محاضرة solution used in medical laboratory كان فيه جزئية طبعا انه هي بدها تفاعل و بدها شرح يعني على سبورة او على ورق لحتى توضح الفكرة فانا حكيتلكم انه راح أصورلكم محاضرة أخرى كملحق لإلها علشان انه تدرسوهم مع بعض و توضح الفكرة خصوصا في جزئية dilution", "tokens": [29973, 5016, 3555, 995, 20328, 3555, 995, 25528, 8032, 1211, 10721, 1975, 5016, 8315, 5296, 15042, 13412, 2288, 5016, 8315, 3714, 5016, 46958, 25720, 3827, 1143, 294, 4625, 16523, 25961, 8978, 3224, 10874, 11622, 19986, 10632, 23032, 3555, 3615, 995, 16472, 3224, 39896, 47525, 11296, 6055, 5172, 995, 30241, 4032, 47525, 11296, 13412, 2288, 5016, 37495, 22653, 15844, 8608, 3555, 13063, 3660, 1975, 2407, 15844, 4032, 2288, 4587, 5296, 33753, 7578, 33427, 11242, 5016, 27188, 4117, 25720, 6156, 7649, 995, 11331, 4117, 36081, 23275, 2304, 16472, 3224, 12602, 39319, 5551, 9381, 13063, 23275, 2304, 3714, 5016, 46958, 25720, 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تناقشوني بالاشياء اللي انتوا مش فاهمينها او اللي مش قادرين تفهموها طبعا عشان اقدر اجاوبكم عليها و نعالج الاشي اول بأول الدراسة اول بأول و المراجعة و انك انت تثبت المعلومات هذا بفيدك اكتر و ان شاء الله يعني هيك ما بيكون اشي عليكم صعب يعطيكم الف عافية و مفقين بإذن الله", "tokens": [2304, 5016, 46958, 25720, 9673, 8315, 4587, 8592, 3660, 15844, 2423, 20742, 1677, 7251, 20498, 21136, 3555, 2655, 7251, 30544, 8978, 11296, 16472, 2655, 14407, 6055, 8315, 4587, 8592, 11536, 1829, 20666, 33599, 1829, 16606, 13672, 1829, 16472, 2655, 14407, 37893, 6156, 995, 16095, 9957, 11296, 1975, 2407, 13672, 1829, 37893, 12174, 18513, 2288, 9957, 6055, 5172, 16095, 2407, 11296, 23032, 3555, 3615, 995, 6225, 8592, 7649, 1975, 28543, 2288, 1975, 7435, 995, 37746, 24793, 25894, 11296, 4032, 8717, 3615, 6027, 7435, 2423, 33599, 1829, 1975, 12610, 4724, 10721, 12610, 32748, 23557, 3794, 3660, 1975, 12610, 4724, 10721, 12610, 4032, 9673, 2288, 26108, 27884, 4032, 16472, 4117, 16472, 2655, 6055, 12984, 3555, 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"end": 3484.22, "word": " اللي", "probability": 0.819091796875}, {"start": 3484.22, "end": 3484.34, "word": " مش", "probability": 0.99365234375}, {"start": 3484.34, "end": 3484.72, "word": " قادرين", "probability": 0.9017333984375}, {"start": 3484.72, "end": 3485.42, "word": " تفهموها", "probability": 0.982421875}, {"start": 3485.42, "end": 3486.06, "word": " طبعا", "probability": 0.745758056640625}, {"start": 3486.06, "end": 3486.68, "word": " عشان", "probability": 0.9498697916666666}, {"start": 3486.68, "end": 3487.06, "word": " اقدر", "probability": 0.8605143229166666}, {"start": 3487.06, "end": 3487.8, "word": " اجاوبكم", "probability": 0.8759765625}, {"start": 3487.8, "end": 3488.36, "word": " عليها", "probability": 0.9912109375}, {"start": 3488.36, "end": 3488.54, "word": " و", "probability": 0.9912109375}, {"start": 3488.54, "end": 3489.0, "word": " نعالج", "probability": 0.936767578125}, {"start": 3489.0, "end": 3489.36, "word": " الاشي", "probability": 0.8556315104166666}, {"start": 3489.36, "end": 3489.64, "word": " اول", "probability": 0.7095947265625}, {"start": 3489.64, "end": 3490.08, "word": " بأول", "probability": 0.8444010416666666}, {"start": 3490.08, "end": 3490.78, "word": " الدراسة", "probability": 0.957763671875}, {"start": 3490.78, "end": 3491.0, "word": " اول", "probability": 0.911865234375}, {"start": 3491.0, "end": 3491.36, "word": " بأول", "probability": 0.9479166666666666}, {"start": 3491.36, "end": 3491.5, "word": " و", "probability": 0.75390625}, {"start": 3491.5, "end": 3492.28, "word": " المراجعة", "probability": 0.97412109375}, {"start": 3492.28, "end": 3492.9, "word": " و", "probability": 0.98291015625}, {"start": 3492.9, "end": 3493.36, "word": " انك", "probability": 0.82568359375}, {"start": 3493.36, "end": 3493.54, "word": " انت", "probability": 0.926513671875}, {"start": 3493.54, "end": 3493.92, "word": " تثبت", "probability": 0.941650390625}, {"start": 3493.92, "end": 3494.66, "word": " المعلومات", "probability": 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\ No newline at end of file
diff --git a/PL9fwy3NUQKwY9jl8xw-bWkVZkWcGGyspd/-PkMjabV1O4_raw.srt b/PL9fwy3NUQKwY9jl8xw-bWkVZkWcGGyspd/-PkMjabV1O4_raw.srt
new file mode 100644
index 0000000000000000000000000000000000000000..f93432a1b8b29ecfadb603278a301425eeeeebb3
--- /dev/null
+++ b/PL9fwy3NUQKwY9jl8xw-bWkVZkWcGGyspd/-PkMjabV1O4_raw.srt
@@ -0,0 +1,2908 @@
+1
+00:00:03,490 --> 00:00:07,770
+مرحبا صبايا هلأ احنا لما شرحنا محاضرة solution
+
+2
+00:00:07,770 --> 00:00:11,530
+used in medical laboratory كان فيه جزئية طبعا انه
+
+3
+00:00:11,530 --> 00:00:18,210
+هي بدها تفاعل و بدها شرح يعني على سبورة او على ورق
+
+4
+00:00:18,210 --> 00:00:23,330
+لحتى توضح الفكرة فانا حكيتلكم انه راح أصورلكم
+
+5
+00:00:23,330 --> 00:00:28,130
+محاضرة أخرى كملحق لإلها علشان انه تدرسوهم مع بعض و
+
+6
+00:00:28,130 --> 00:00:30,410
+توضح الفكرة خصوصا في جزئية dilution
+
+7
+00:00:41,500 --> 00:00:47,020
+طبعا أنا بدأت بال .. بالجزئية الأولى للمحاضرة يعني
+
+8
+00:00:47,020 --> 00:00:52,200
+حكيت عن .. عن المحاليل و أنواعها و شرحت بعض
+
+9
+00:00:52,200 --> 00:00:56,610
+الأفكار عنهاو بعدين حكينا عن الوحدات القياس و من
+
+10
+00:00:56,610 --> 00:01:01,270
+ثم ال dilution فبرضه هذه المحاضرة شاملة و يعني
+
+11
+00:01:01,270 --> 00:01:06,250
+المهم أنه تدرسوها جنب تسجيل المحاضرة الأساسي اللي
+
+12
+00:01:06,250 --> 00:01:09,810
+نزل على ال model طبعا هلأ أنا راح أبلش أشغل
+
+13
+00:01:09,810 --> 00:01:14,390
+الفيديو هيبدأ يعرضلكم الفيديو اللي مصور أصلا سابق
+
+14
+00:01:14,390 --> 00:01:18,750
+مسبقا وبيشرح المحاضرة السلام عليكم ورحمة الله
+
+15
+00:01:18,750 --> 00:01:23,920
+وبركاته طالبتيقليبة مستوى .. قليبة مستوى التاني
+
+16
+00:01:23,920 --> 00:01:28,200
+قصة معلم طبية مقبلية أهلا وسهلا بكم ان شاء الله
+
+17
+00:01:28,200 --> 00:01:34,480
+اللي رح اليوم نعطي تاكملة المحاضرة اللي حكينا رح
+
+18
+00:01:34,480 --> 00:01:38,780
+نشرحها على ورق مقدر بالأمثلة لحتى انعمق الفهم
+
+19
+00:01:38,780 --> 00:01:42,890
+للمحاضرة المسجلةكانت المحاضرة تنبيع منها solution
+
+20
+00:01:42,890 --> 00:01:46,450
+used in medical laboratories وحكينا في المحاضرة
+
+21
+00:01:46,450 --> 00:01:49,210
+أنه انا فيها من ياله بدرة أنواع من المحاليات
+
+22
+00:01:49,210 --> 00:01:53,170
+المستخدمة في المختبرات الطبية، من هذه المحاليات
+
+23
+00:01:53,170 --> 00:01:58,700
+الرئيسات أربع أنواعالصبغات البقهور و ال standard
+
+24
+00:01:58,700 --> 00:02:02,860
+حكينا عن ال reagent هو اللى بيعملى ال change طبعا
+
+25
+00:02:02,860 --> 00:02:06,180
+هو المادة المتفاعلة اللى بتضيقها على العينة بتعملى
+
+26
+00:02:06,180 --> 00:02:12,680
+change تغير measurable or noticable يعني أما يكون
+
+27
+00:02:12,680 --> 00:02:17,840
+يمكن قياسه أو يمكن ملاحظته هذا التغير اللى يحدثه
+
+28
+00:02:17,840 --> 00:02:21,740
+على العينة بنجرد أنه يفتح على العينة للكشف على
+
+29
+00:02:21,740 --> 00:02:27,310
+مادة معينة أو يعني يعنيعن جزء من العيلة مراقية او
+
+30
+00:02:27,310 --> 00:02:31,730
+مراد فحصه النوع الآخر من المحاليل كان هو الصبغات
+
+31
+00:02:31,730 --> 00:02:35,230
+النوع الثاني من المحاليل حكيناه اللي هو الصبغات
+
+32
+00:02:35,230 --> 00:02:40,310
+اللي بيعطيني مكون من طبعا من colored dyes اللي هي
+
+33
+00:02:40,310 --> 00:02:44,570
+صبغات ملونة تكسب العيلة artificial color او لون
+
+34
+00:02:44,570 --> 00:02:48,090
+مكتسب او لون استماعى لحتى يتمكن من رؤيتها تحت ال
+
+35
+00:02:48,090 --> 00:02:51,780
+microscopeالنوع التالت من المحاليل كان البحر اللي
+
+36
+00:02:51,780 --> 00:02:55,660
+هو المحلول الضعبة للبيئة هذا النوع من المحاليل
+
+37
+00:02:55,660 --> 00:02:59,800
+control بيئة هذا وظيفته ومن خصائصه ان هو resist
+
+38
+00:02:59,800 --> 00:03:04,400
+change البيئة بقالم التغير في البيئة النوع الرابع
+
+39
+00:03:04,400 --> 00:03:07,640
+اللي هو ال standard طبعا هحكيله مش بالترتيب زي ما
+
+40
+00:03:07,640 --> 00:03:12,700
+هم بالمحاضرة فيعني لأ بس لذكر ال standards هو
+
+41
+00:03:12,700 --> 00:03:16,840
+المحاليل القياسيةطبعا ال standard هي عبارة عن
+
+42
+00:03:16,840 --> 00:03:20,940
+محالي قياسية او ميارية او اهم ما يميزها انها non
+
+43
+00:03:20,940 --> 00:03:25,880
+-concentration طبعا non-concentration تاكيز معلوم
+
+44
+00:03:25,880 --> 00:03:31,040
+طبعا احنا حكينا انه استخدام ال standard solution
+
+45
+00:03:31,040 --> 00:03:36,120
+انه انا لان تاكيزها بيكون معلوم بجيب تاكيز مشهور
+
+46
+00:03:36,120 --> 00:03:41,640
+في العينة بدلت هذا المعلوم كيف يحدث هذا؟لو فرضت
+
+47
+00:03:41,640 --> 00:03:45,580
+انه انا في عندى عينة فيها سامل في .. طبعا النبو
+
+48
+00:03:45,580 --> 00:03:50,820
+فيها عينة و النبو اخر فيه محلول standard طبعا اللي
+
+49
+00:03:50,820 --> 00:03:53,740
+لو انا بدي اقس مستوى السكر في الدم بدي اقس ال
+
+50
+00:03:53,740 --> 00:03:57,700
+glucose اللي موجود في الدموهذا المحلول طبعا أكيد
+
+51
+00:03:57,700 --> 00:04:00,560
+هيكون محلول glucose معلوم التركيز عشان انا اقرأ
+
+52
+00:04:00,560 --> 00:04:04,340
+نوع العيلة مابنفعش مثلا انا اشأ بدي اقلس السكر
+
+53
+00:04:04,340 --> 00:04:07,660
+اجيب ال standard محلول البروتين لأ لازم يكون من
+
+54
+00:04:07,660 --> 00:04:12,220
+نفس النوع علشان انا استخدمه ك .. كمحلول معلوم
+
+55
+00:04:12,220 --> 00:04:17,300
+التركيز و اجيب المجهول بدلالتهطبعا هذا بالنسبة لمن
+
+56
+00:04:17,300 --> 00:04:22,700
+لا ال .. السامبل بدي ا .. ا .. اشوف ال .. بدي اقيس
+
+57
+00:04:22,700 --> 00:04:26,160
+تاركيز من ال glucose اللي موجود فيها و الاسفلين دي
+
+58
+00:04:26,160 --> 00:04:29,200
+محلول استعداد اقل موجود في ال glucose محلول اللي
+
+59
+00:04:29,200 --> 00:04:32,920
+هو معاهم التاركيز من ال glucose بدي انا من خلال ال
+
+60
+00:04:32,920 --> 00:04:40,410
+..التجربة أستخدمه كمحلول مياري أو تياسي أول اشي
+
+61
+00:04:40,410 --> 00:04:43,410
+بدي أعمله بدي أضيف ال reagent هو اللي هي المادة
+
+62
+00:04:43,410 --> 00:04:47,490
+المتفاعلة على كل مانع ال simple و ال standard راح
+
+63
+00:04:47,490 --> 00:04:50,850
+أضيف ال reagent المادة المتفاعلةطبعا المادة
+
+64
+00:04:50,850 --> 00:04:54,050
+المتفاعلة دهالى او اللى .. اللى راح تعطينى change
+
+65
+00:04:54,050 --> 00:04:58,070
+راح تعطينى مجارب organic metabolic change طبعا
+
+66
+00:04:58,070 --> 00:05:02,250
+هتتفاعل مع ال glucose و تعطينى تغير تغير ممكن يكون
+
+67
+00:05:02,250 --> 00:05:06,430
+فى اللون او كده احنا درمنا مثال انه اذا انا رفض
+
+68
+00:05:06,430 --> 00:05:11,670
+انه في ال .. في ال .. فرد تغير اللون و يعطى لي لون
+
+69
+00:05:11,670 --> 00:05:17,030
+pink مثلا لو مثلا هانا اعطانى لون pink تمام و هانا
+
+70
+00:05:17,030 --> 00:05:23,410
+اعطانى ايضا لون pink اخرعلى فرض أنه التكييز هنا
+
+71
+00:05:23,410 --> 00:05:27,330
+عبارة عن 100 milligram بالديسيلتر بالنسبة لل
+
+72
+00:05:27,330 --> 00:05:32,750
+standard تمام؟ 100 milligram بالديسيلتر و أنا دخلت
+
+73
+00:05:32,750 --> 00:05:35,590
+الإبعاد اللي متستخدم بالها دي و ال standard عادي
+
+74
+00:05:35,590 --> 00:05:39,590
+هذه تقيس الشد كلون على فرض أنه أعطاني الشد كلون
+
+75
+00:05:39,590 --> 00:05:44,090
+كانت اتنين و أنا أعطاني واحدطبعا إذا هنا شدت اللون
+
+76
+00:05:44,090 --> 00:05:48,730
+نصف شدت اللون لل standard معناه إيه التركيز إذا
+
+77
+00:05:48,730 --> 00:05:52,470
+كان هنا مياه معناه يكون التركيز هنا خمسين على فرض
+
+78
+00:05:52,470 --> 00:05:58,890
+أنه أعطاني .. على فرض أنه أعطاني شدت لون أقوى هنا
+
+79
+00:05:58,890 --> 00:06:04,660
+بينيطبعا ف .. و الجهاز قاسها على أساس أنها هي
+
+80
+00:06:04,660 --> 00:06:10,680
+أربعة طبعا و هنا أتنين فلنفرض أنه الأتنين كانت
+
+81
+00:06:10,680 --> 00:06:13,540
+تاركيزها مية طبعا الأتنين كانت تاركيزها مية
+
+82
+00:06:13,540 --> 00:06:16,100
+الأربعة كانت راح تكون تاركيزها .. راح تكون
+
+83
+00:06:16,100 --> 00:06:19,740
+تاركيزها متين، اللي هو الدقف فبالتالي بهذا الشكل
+
+84
+00:06:19,740 --> 00:06:24,640
+أو بهذا المبدأ أنا بجيب تاركيز المواد أو المكونات
+
+85
+00:06:24,640 --> 00:06:29,700
+للعينة طبعا المشهودةبالدالات معلوقة لو هو ال
+
+86
+00:06:29,700 --> 00:06:32,900
+standard، هاد .. هاد فكرة استخدام ال standard
+
+87
+00:06:32,900 --> 00:06:36,480
+solution في معرفة التركيز .. التركيز المشغولة،
+
+88
+00:06:36,480 --> 00:06:40,480
+طبعا، و هي دور ال agent، دور ال agent هو يكشف أنه
+
+89
+00:06:40,480 --> 00:06:44,900
+يعطيه change، يعطيه تغيرطبعا؟ ال .. ال .. ال ..
+
+90
+00:06:44,900 --> 00:06:48,260
+إذا هك خلصنا و شرحنا ال reagent و ال standard
+
+91
+00:06:48,260 --> 00:06:51,600
+أكتر، ال stem حاكينا أن هو عبارة عن صبغة غلطي
+
+92
+00:06:51,600 --> 00:06:56,020
+مكونة من color dye و بت .. تكسب ال .. ال .. مكونات
+
+93
+00:06:56,020 --> 00:06:59,720
+العينا artificial color لحتى نتمكن من رؤيتها تحت
+
+94
+00:06:59,720 --> 00:07:03,580
+ال microscope من خلال هذه الصبغات أنا بتمكن رؤية
+
+95
+00:07:03,580 --> 00:07:08,720
+العينات بشكل أضح و مكوناتها سواء كانت خلايا، أملح،
+
+96
+00:07:08,720 --> 00:07:15,070
+مايكروباتبكتيريا فطريات وفيليات كل أنواع المكونات
+
+97
+00:07:15,070 --> 00:07:18,150
+لدهر دهر الخلية ممكن سباطها و رؤيتها تحت ال
+
+98
+00:07:18,150 --> 00:07:22,250
+microscope بالنسبة للبفر هو عبارة عن محلول حكينا
+
+99
+00:07:22,250 --> 00:07:26,390
+بقى بتلالي أش يقوم تراير في اللي أش وحكينا أن هو
+
+100
+00:07:26,390 --> 00:07:32,710
+بدي يكون مكوّن من أش إما من weak acid مع ال strong
+
+101
+00:07:32,710 --> 00:07:39,350
+base تبعتهأو ال strong acid و ال weak base تبعته و
+
+102
+00:07:39,350 --> 00:07:43,810
+base فى المنظمة شرحلكم ان هو ممكن يكون مقوم من ال
+
+103
+00:07:43,810 --> 00:07:47,350
+weak acid و ال weak source تبعته هى نفس المعنى
+
+104
+00:07:47,350 --> 00:07:51,710
+خلينا نضرب مثلا اللى هى عندها C-H-D-C-O-A-S اللى
+
+105
+00:07:51,710 --> 00:07:56,790
+هو عبارة عن هذا acidic acid تمام؟ acidic acid ال
+
+106
+00:07:56,790 --> 00:08:01,510
+acidic acid هذا عبارة عن weak acid تمام؟ممكن انا
+
+107
+00:08:01,510 --> 00:08:06,830
+اكون بطار باستخدامه مع وجود ال CH3COO مالك اللى هو
+
+108
+00:08:06,830 --> 00:08:10,330
+الايونات التفارق تبعه الايونات التفارق تبعه هذا
+
+109
+00:08:10,330 --> 00:08:14,950
+بسميه acetate تمام و هو عبارة عن ملح و هو اللى
+
+110
+00:08:14,950 --> 00:08:20,660
+خلاص انه strong baseطبعا هو متأين بيكون له خاصة
+
+111
+00:08:20,660 --> 00:08:24,840
+strong base هدول المع بعض بيكونوا متبقار اذا اما
+
+112
+00:08:24,840 --> 00:08:28,060
+weak acid و strong base او weak acid و ال .. و ال
+
+113
+00:08:28,060 --> 00:08:31,760
+.. و ال konjugate base او weak acid و ال salt تبعه
+
+114
+00:08:31,760 --> 00:08:36,340
+نفس المعنى طبعا هذا بالنسبة للمعنى الحليب و
+
+115
+00:08:36,340 --> 00:08:41,680
+المكوناتطبعا احنا شريحنا بالنسبة لمحالين هذه ايضا
+
+116
+00:08:41,680 --> 00:08:44,880
+الاعتبارات اللي تتطرق طرحها أثناء استخدامها أو
+
+117
+00:08:44,880 --> 00:08:49,420
+أثناء تحضيرها ويبقى علومات عن كيفية الحفظ والخواص
+
+118
+00:08:49,420 --> 00:08:53,780
+اللي لازم تتوفر فيها علشان انا اعتبرها سواء كانت
+
+119
+00:08:53,780 --> 00:08:56,960
+the end او اعتبرها standard او اعتبرها بفر وهكذا
+
+120
+00:08:57,430 --> 00:09:01,410
+طيب بعد هيك حكينا .. طبعا حكينا عن أن هو ال
+
+121
+00:09:01,410 --> 00:09:04,330
+standard solution أنه primary standard و secondary
+
+122
+00:09:04,330 --> 00:09:09,210
+standard و في النهاية حكينا أنه المحليل هذه لما
+
+123
+00:09:09,210 --> 00:09:14,830
+أنا بدي أعبر عن التركيز تبعها لازم أنا أستخدم
+
+124
+00:09:14,830 --> 00:09:20,930
+التركيز أحكي ال concentration و ال unit تبعته طبعا
+
+125
+00:09:20,930 --> 00:09:25,130
+أحكي عن ال concentration و ال unitبنفعش مثلا أحكي
+
+126
+00:09:25,130 --> 00:09:28,510
+وضع المحلول، ضربنا مثلا في المحاضرة المسجلة، أقول
+
+127
+00:09:28,510 --> 00:09:32,250
+إن أنا بنفعش أنا أجيب محلول تركيزه خمسين و أقول
+
+128
+00:09:32,250 --> 00:09:37,330
+إنه خمسين أو بس، طب خمسين أيش؟ طب يعني .. طب يعني
+
+129
+00:09:37,330 --> 00:09:42,650
+أنا بيأفهم هو .. هو يعني بأي وحدة علشان أعرف مدى
+
+130
+00:09:42,650 --> 00:09:47,310
+قوة التركيز تبعه، فبالتالي إنه لازم أنا أعبر عن ال
+
+131
+00:09:47,310 --> 00:09:51,350
+.. ال .. المحلول ب .. ال concentration بالرقم و ال
+
+132
+00:09:51,350 --> 00:09:55,690
+unitطبعا وحاكينا أنه في عندي نوعين من ال units من
+
+133
+00:09:55,690 --> 00:10:01,070
+الوحدات تستخدم للتعبير عن ال concentration في نوع
+
+134
+00:10:01,070 --> 00:10:08,950
+اسمه physical طبعا physical units ونوع آخر بنسميه
+
+135
+00:10:08,950 --> 00:10:15,580
+اللي هو ال chemical unitsطبعا؟ طيب، ماشي بنحكيلها
+
+136
+00:10:15,580 --> 00:10:18,340
+على ال physical يعني نسمي اسمها وحدة كيزيائية يعني
+
+137
+00:10:18,340 --> 00:10:25,800
+بنعبر بالوزن مثلا بالجراح، الطول مثلا ال length
+
+138
+00:10:25,800 --> 00:10:26,580
+بال centimeter
+
+139
+00:10:29,290 --> 00:10:33,810
+الحجم بالأقل هذه الوحدات النهائية اللي أنتوا
+
+140
+00:10:33,810 --> 00:10:38,050
+أخدتوها أكيد يعني أعتقد من طول مرات معاكم في مصارخ
+
+141
+00:10:38,050 --> 00:10:43,410
+الفيزيا فطبعا لو في عندي ثلاث أنواع من الوحدات
+
+142
+00:10:43,410 --> 00:10:50,110
+اللي هم weight to volumeو weight to weight او
+
+143
+00:10:50,110 --> 00:10:55,150
+weight per volume weight per weight و volume per
+
+144
+00:10:55,150 --> 00:10:59,030
+volume انا بحكيها weight to volume ratio نفس
+
+145
+00:10:59,030 --> 00:11:01,390
+الكلام يعني لو هو weight to volume او weight per
+
+146
+00:11:01,390 --> 00:11:05,070
+volume نفس المعنى طيب مفروض ان فيلم دي محلول مثلا
+
+147
+00:11:05,070 --> 00:11:10,340
+هذا المحلول مثلا AACLطبعا محلول مدح الطعام ال NACL
+
+148
+00:11:10,340 --> 00:11:14,580
+هذا تتيزه تلاتين في المية طبعا خلينا نشوف ايش معنى
+
+149
+00:11:14,580 --> 00:11:18,040
+كلمة تلاتين في المية طبعا هو موجود في دست البوتر
+
+150
+00:11:18,040 --> 00:11:24,680
+ال solvent طبعا طيب محلول NACL ال solute عندي NACL
+
+151
+00:11:24,680 --> 00:11:28,800
+طبعا و ال solventالـ distilled water احنا حاكينا
+
+152
+00:11:28,800 --> 00:11:32,360
+انه اي محلول بيكون مكوّن من solute تمام اللي هي
+
+153
+00:11:32,360 --> 00:11:36,800
+المادة المدابة و ال solvent المضيب تمام ال IACA هي
+
+154
+00:11:36,800 --> 00:11:40,500
+المادة المدابة و ال solvent ال distilled water هو
+
+155
+00:11:40,500 --> 00:11:44,340
+المضيب خليني نحكي انه تركيزه تلاتين تلمية خليني
+
+156
+00:11:44,340 --> 00:11:49,060
+نشوف ايش يعني تلاتين تلمية لو تلاتين تلمية لو كانت
+
+157
+00:11:49,060 --> 00:11:55,700
+الوحدة wet volume لو كانت الوحدة تلاتين تلمية wet
+
+158
+00:11:55,700 --> 00:12:02,380
+بال volumeطبعا من ال .. من ال NACL معناها أنا
+
+159
+00:12:02,380 --> 00:12:08,500
+استخدمت تلاتين جرام طبعا هذا .. هذا solute و هذا
+
+160
+00:12:08,500 --> 00:12:14,360
+solvent بيكون طبعا أنا استخدمت تلاتين جرام من ال
+
+161
+00:12:14,360 --> 00:12:20,300
+solute اللي هو H هو عبارة عن HNACL تلاتين جرام
+
+162
+00:12:20,300 --> 00:12:29,230
+طبعا لإنهاءاتطبعا من ال NACL و أخدت كمان مية AML
+
+163
+00:12:29,230 --> 00:12:35,410
+AML لإنفوريا منين؟ من الصولة اللي هو عبارة عن H
+
+164
+00:12:35,410 --> 00:12:40,320
+distilled waterهذا كان في حال لو أنا ساخدم تلاتين
+
+165
+00:12:40,320 --> 00:12:46,240
+كمية weight و volume NACL طيب لو كانت عندي تلاتين
+
+166
+00:12:46,240 --> 00:12:51,080
+كمية weight per volume او sorry weight per weight
+
+167
+00:12:51,080 --> 00:12:57,400
+طبعا NACL اشنا نعمل معناها طبعا احنا عارفين ان هذا
+
+168
+00:12:57,400 --> 00:13:04,350
+لل solute و هذا لل solvent طبعاتلاتين قاش اللويات
+
+169
+00:13:04,350 --> 00:13:10,990
+جرام على سبعين جرام هما ب .. هما بحط تمية كاملة زي
+
+170
+00:13:10,990 --> 00:13:15,210
+اللي ما هي لأ هما بكمل تكميل بحث أنه يكون ال total
+
+171
+00:13:15,210 --> 00:13:20,550
+تمام عندي مية اللي هي أنا مو نجيب تمية هذه هي مية
+
+172
+00:13:20,550 --> 00:13:25,000
+تمية تمام؟ انا من النسبة المقلوية اللي هناهذا
+
+173
+00:13:25,000 --> 00:13:28,860
+معناها انه لما اقول تلاتين في المية wet و بقى bare
+
+174
+00:13:28,860 --> 00:13:34,780
+wet لما احكي تلاتين في المية .. لما احكي تلاتين في
+
+175
+00:13:34,780 --> 00:13:40,540
+المية طبعا wet او sorry volume .. bare volume ايش
+
+176
+00:13:40,540 --> 00:13:46,300
+معناه هذا للsolute تلاتين ml طبعا و هذا لل solvent
+
+177
+00:13:47,500 --> 00:13:52,440
+70 ml اكمل التكميل بحكيتلكم في المحاضرة المسجلة
+
+178
+00:13:52,440 --> 00:13:56,340
+انه لما يكون الوحدات مختلفة انا بحط ال 30 زي ما هي
+
+179
+00:13:56,340 --> 00:13:59,800
+و ال 100 زي ما هي لما يكون الوحدات متشابهة weight
+
+180
+00:13:59,800 --> 00:14:02,460
+per weight volume per volume انا بكمل التكميل
+
+181
+00:14:02,460 --> 00:14:08,000
+لانهية يعني 30 gram هيكون من ال NACL 70 gram و ليس
+
+182
+00:14:08,000 --> 00:14:11,280
+ml من ال distilled water لإن انا بحكي weight per
+
+183
+00:14:11,280 --> 00:14:16,540
+weight لما احكي volume per volume بدي اقول 30 mlمن
+
+184
+00:14:16,540 --> 00:14:21,500
+الان محلول NACL و سبعين ML من السيوت اللي بتقوله
+
+185
+00:14:21,500 --> 00:14:26,220
+distilled water هذا معناه ان السيوت اللي انا
+
+186
+00:14:26,220 --> 00:14:30,480
+بستخدمه ممكن يكون مادة بودر او زيتها تمام ممكن
+
+187
+00:14:30,480 --> 00:14:34,220
+يكون عبارة عن محلول بدقة خفيفةطبعا solution بدي
+
+188
+00:14:34,220 --> 00:14:39,220
+أخففها طبعا فإذا هي زينا أخذت تلاتين أقل من ACL
+
+189
+00:14:39,220 --> 00:14:43,200
+هتقول طب ال NACL محتاجة كده أوي انه تلاتين أقل
+
+190
+00:14:43,200 --> 00:14:46,400
+بيكون البرأة محلول وانا بدي أكمله لسبعين عشان أصل
+
+191
+00:14:46,400 --> 00:14:51,720
+لتركيز تلاتين تلمية بدي أخففطيب بالنسبة لـ
+
+192
+00:14:51,720 --> 00:14:54,760
+chemical units حكي ان انا بعبر بالspecific gravity
+
+193
+00:14:54,760 --> 00:14:58,820
+و ممكن اعبر بواحد تانية اللي هم ال molarity و ال
+
+194
+00:14:58,820 --> 00:15:02,240
+normality ال molarity عبارة عن ايه في ال molarity
+
+195
+00:15:02,240 --> 00:15:08,580
+قانونها عبارة عن number of moles على ال volume per
+
+196
+00:15:08,580 --> 00:15:13,380
+liter خليني اختار ان انا مثلا عندي اتنين إجراء من
+
+197
+00:15:13,380 --> 00:15:18,500
+ال S2 و S04 المولارتي بتاعتهم جديش بتاعتهم تمام؟
+
+198
+00:15:18,640 --> 00:15:23,760
+خلّينا نشوف هنا اتنين جرام من الـ H2O4 كم الولادة
+
+199
+00:15:23,760 --> 00:15:27,140
+تبعتها بتطمون طبعا ال number of moles هنا يا بنات
+
+200
+00:15:27,140 --> 00:15:30,640
+عبارة عن إيش؟ عبارة عن ال weight على الملوك اللي
+
+201
+00:15:30,640 --> 00:15:37,320
+لرويت طبعا؟ طيب اتنين جرام على الملوك اللي لرويت
+
+202
+00:15:37,320 --> 00:15:43,120
+للـ H2O4 98 سنة جدا نحسبهاعشان اشتغل الوحدة اتنين
+
+203
+00:15:43,120 --> 00:15:47,120
+على تمانية وتسعين هذه ال number of moles على ال
+
+204
+00:15:47,120 --> 00:15:49,960
+volume ب one liter طبعا محدد ب one liter انا اخترت
+
+205
+00:15:49,960 --> 00:15:56,420
+على one liter بحسب جديش ال morality تبعتهاللي
+
+206
+00:15:56,420 --> 00:16:00,440
+بتطلع معايا طبعا إذا ال molarity بنعيدها تاني
+
+207
+00:16:00,440 --> 00:16:07,160
+تساوي number of moles على volume بالliter حاجة
+
+208
+00:16:07,160 --> 00:16:11,280
+بالliter طبعا ويساوي ال number of moles هذه إيش
+
+209
+00:16:11,280 --> 00:16:15,300
+بتساوي بتساوي ال weight على ال molecule ال weight
+
+210
+00:16:15,300 --> 00:16:22,050
+على كله طبعا هذا هو كله على ال volume بالliterكانت
+
+211
+00:16:22,050 --> 00:16:27,650
+عندي وقص وقص وفور و وزنتها جدش و اتنين جرام تمام
+
+212
+00:16:27,650 --> 00:16:32,290
+اتنين جرام على اللي مالك فيه قاروة جدش و وزنتها
+
+213
+00:16:32,290 --> 00:16:38,680
+تمانية و تسعين طبعا جرام برمولمش تملك الارواح على
+
+214
+00:16:38,680 --> 00:16:43,600
+ال volume per liter one liter فبحسبها جدش تطلع
+
+215
+00:16:43,600 --> 00:16:48,440
+عندى ال .. ال .. ال .. ال molarity طبعا هذا
+
+216
+00:16:48,440 --> 00:16:51,760
+بالنسبالى ال molarity طب ال normality ال normality
+
+217
+00:16:51,760 --> 00:16:55,620
+ماتيكيها بال slide عبارة عن ايش تملكي الارواح على
+
+218
+00:16:55,620 --> 00:17:00,620
+ال N ال N هنا هي عبارة عن ال equivalence ال
+
+219
+00:17:00,620 --> 00:17:06,050
+equivalence طبعا اشي عن equivalence هو تكافأةطبعا
+
+220
+00:17:06,050 --> 00:17:13,170
+يعني مثلا الأس و أس أخر لما بيتأين اش بيعطيني
+
+221
+00:17:13,170 --> 00:17:18,710
+بيعطيني أس أخر ساعة اتنين تكاعف أتنين إذا ال .. ال
+
+222
+00:17:18,710 --> 00:17:21,170
+.. ال normality لها ده المحلول هتكون ال molecular
+
+223
+00:17:21,170 --> 00:17:24,850
+weight تبعين وتسعين على اتنين و يساو تسعة و أربعين
+
+224
+00:17:26,410 --> 00:17:31,710
+طبعا ايش الوحدات هنا؟ بتطلع هنا بال normal انت
+
+225
+00:17:31,710 --> 00:17:35,170
+تنسى و قدرين normal هنا ال .. ال .. ال molarity
+
+226
+00:17:35,170 --> 00:17:39,650
+لما انا احسبها بتطلع عندى العرض و وحدة القياس
+
+227
+00:17:39,650 --> 00:17:44,430
+molar طبعا لإنها molarity هذه هي ال chemical units
+
+228
+00:17:44,430 --> 00:17:51,310
+اذا حكينا عن المحارير اللى بتستخدم في ال ..في
+
+229
+00:17:51,310 --> 00:17:57,190
+المقتورات الطبية وحكينا كيف انا بقارن ال .. بجيب
+
+230
+00:17:57,190 --> 00:18:00,170
+التركيز المجرور في العينة بدلالة ال standard ايش
+
+231
+00:18:00,170 --> 00:18:05,410
+الفكرة بالظبط وحكينا ايضا عن الوحدات اللي هي ال
+
+232
+00:18:05,410 --> 00:18:09,470
+physical units و ال chemical units و التطبيق عليها
+
+233
+00:18:09,470 --> 00:18:14,090
+تمام؟ بعد هي انتقلنا لحتى نحكي عن ال dilution
+
+234
+00:18:14,090 --> 00:18:19,410
+تمام؟ في المحاضرة المسجلة ال dilution التخليف
+
+235
+00:18:19,980 --> 00:18:24,480
+دالبتلكم مثال حكيتلكم انه لو انتي عندك كوب حطيتي
+
+236
+00:18:24,480 --> 00:18:30,140
+فيه معلق السكر طبعا خلينا نفترض انك انتي ضختي معلق
+
+237
+00:18:30,140 --> 00:18:35,300
+السكر هنا و حطيتيها و
+
+238
+00:18:35,300 --> 00:18:47,600
+عبيتي لنصها ماء طبعا فهنا سكر sugar حطيه هنا طيبو
+
+239
+00:18:47,600 --> 00:18:51,620
+احنا حاطينا نفس المعالقة نفس الكمية و انا عبيتها
+
+240
+00:18:51,620 --> 00:18:59,020
+لآخر ماء طبعا اذا .. اذا ال solute دي اسمها عبارة
+
+241
+00:18:59,020 --> 00:19:05,380
+عن sugar و ال solvent من distilled water بس انا
+
+242
+00:19:05,380 --> 00:19:11,840
+عبيت لنص و انا عبيت لكل الكاسة فاليامين رح يكون
+
+243
+00:19:13,740 --> 00:19:18,460
+طبعا اكيد هذا طبعا هيكون اللي هيدي sweet اكتر
+
+244
+00:19:18,460 --> 00:19:23,340
+sweet او اللي هيدي more sweet هذا لسه sweet هيكون
+
+245
+00:19:23,340 --> 00:19:24,060
+صح؟
+
+246
+00:19:26,640 --> 00:19:32,800
+ليش؟ لإن أنا هتضفت من ال solvent أكتر، هتخفف أكتر
+
+247
+00:19:32,800 --> 00:19:40,740
+طيب أصبح التخفيف هو عبارة عن إن أنا أقلل من ال
+
+248
+00:19:40,740 --> 00:19:45,040
+concentration من خلال إن أنا إيش أعمل؟ أضيف
+
+249
+00:19:45,040 --> 00:19:49,620
+solvent وهذا هو التعريف اللي موجود في الفيديو هو
+
+250
+00:19:49,620 --> 00:19:55,800
+إن أنا أعمل decrease لال concentration من خلال ان
+
+251
+00:19:55,800 --> 00:20:01,140
+انا addition اعمل addition لانهي ضعف المزيد من ال
+
+252
+00:20:01,140 --> 00:20:05,320
+solvent هذا تعريف ال dilution طبعا وحاكينا ان ال
+
+253
+00:20:05,320 --> 00:20:09,620
+dilution يستخدم كتير في المختبرات التدبية في تحديد
+
+254
+00:20:09,620 --> 00:20:15,390
+المحاليل فلازم احنا نستوعب فكرة انهحكينا ان انا في
+
+255
+00:20:15,390 --> 00:20:20,790
+عندي نوعين من ال dilution نوع بنسميه simple
+
+256
+00:20:20,790 --> 00:20:26,390
+dilution، dilution بسيط و نوع اخر بنسميه ال
+
+257
+00:20:26,390 --> 00:20:32,510
+dilution ال cereal، ال cereal dilution إيش يعني
+
+258
+00:20:32,510 --> 00:20:38,810
+simple dilution؟ يعني بخطوة واحدة بيصير عدة
+
+259
+00:20:38,810 --> 00:20:40,370
+multiple
+
+260
+00:20:44,230 --> 00:20:48,650
+steps و طبعا موجودة كلمة multiplier بالصلاة ده
+
+261
+00:20:48,650 --> 00:20:56,390
+multiplicator progressive steps طبعا هذه عدة خطوات
+
+262
+00:20:56,390 --> 00:20:59,410
+مع
+
+263
+00:20:59,410 --> 00:21:03,230
+السلامة يا عزيزي يا عزيزي يا عزيزيعدد خطوات طبعا
+
+264
+00:21:03,230 --> 00:21:08,030
+هذا الفرق ما بين اثنين بالوثيوب يعني طيب اش يعني
+
+265
+00:21:08,030 --> 00:21:11,850
+يعني مثلا لما انا يكون في عندي مادة بسميها خلينا
+
+266
+00:21:11,850 --> 00:21:15,510
+نسميها stock او اسميها original المادة الأصلية
+
+267
+00:21:15,510 --> 00:21:20,510
+اللي انا بده اخففها لو انا أخدت منها volume طبعا
+
+268
+00:21:20,510 --> 00:21:26,170
+وحطيته في ال tube تبعتي طبعا واضغطت هنا solvent
+
+269
+00:21:26,170 --> 00:21:32,990
+ماعرفش انا بعد ما أبقى عافيةعدة خطوات طبعا هذا
+
+270
+00:21:32,990 --> 00:21:38,470
+الفرق ما بين سنة بالوسيلية طيب اش يعني يعني مثلا
+
+271
+00:21:38,470 --> 00:21:41,430
+لما انا يكون في نعدي مادة بسميها خلينا نسميها
+
+272
+00:21:41,430 --> 00:21:44,970
+stock او بسميها original المادة الأصلية اللي انا
+
+273
+00:21:44,970 --> 00:21:50,710
+بده اخففها لو انا أخدت منها volume طبعا وحاطلته في
+
+274
+00:21:50,710 --> 00:21:58,420
+ال tube تبعتي طبعا واضفت هنا solventمعناها انا هنا
+
+275
+00:21:58,420 --> 00:22:01,760
+بخطوة واحدة يعني ان انا بصي .. نقلت منها لتيوب و
+
+276
+00:22:01,760 --> 00:22:06,200
+نقلت solvent هذا بسميه simple لكن ال serial ان انا
+
+277
+00:22:06,200 --> 00:22:12,500
+هيكون .. يكون عندي stock تمام؟ و انقل من خط .. منه
+
+278
+00:22:12,500 --> 00:22:16,680
+لتيوب و منه ايضا لتيوب اخرى و هكذا لتيوب اخرى
+
+279
+00:22:16,680 --> 00:22:20,940
+فيكون متسامسل يعني معقد اكتر فهذا بسميه serial
+
+280
+00:22:22,130 --> 00:22:28,070
+حكينا معلومة انه ال serial بيبلش ك simple يعني أول
+
+281
+00:22:28,070 --> 00:22:32,950
+خطوة في ال serial هي عبارة عن simple طبعا اول خطوة
+
+282
+00:22:32,950 --> 00:22:39,330
+في ال serial عبارة عن simple بعدين بكمل كل ما
+
+283
+00:22:39,330 --> 00:22:43,130
+اتعقد اكتر بقلط ال simple ال serial اشماله بيصير
+
+284
+00:22:43,130 --> 00:22:46,890
+ال serial و هكذا هاي الفرق ما بين simple dilution
+
+285
+00:22:46,890 --> 00:22:50,450
+و simple serial dilutionهلأ بناخد قمتلة على كل من
+
+286
+00:22:50,450 --> 00:22:54,550
+ال simple dilution و ال serial dilution لحتى نعرف
+
+287
+00:22:54,550 --> 00:22:59,330
+كيف احنا نحل المسائل المتعلقة بهذا ال .. ال ..
+
+288
+00:22:59,330 --> 00:23:04,570
+الشيء ده خلينا نحكي عن اول اشي ال simple dilution
+
+289
+00:23:04,570 --> 00:23:10,010
+simple
+
+290
+00:23:10,010 --> 00:23:10,570
+dilution
+
+291
+00:23:13,350 --> 00:23:18,150
+حكينا ان هو يحدث او يجري بخطوة واحدة يعني مثلا
+
+292
+00:23:18,150 --> 00:23:22,110
+نفرض ان انا في مدينة هناك يوب تركيزها مثلا نتين
+
+293
+00:23:22,110 --> 00:23:29,990
+ميلي جرام بار او قلب تمام؟ فيها محلول يولية تمام؟
+
+294
+00:23:29,990 --> 00:23:34,710
+مهدأ اسمها يولية لو انا بدي اخد منها من هناone
+
+295
+00:23:34,710 --> 00:23:39,750
+أبقال و احطها في تيوب و تيوبها بتاعة تسعة أبقال من
+
+296
+00:23:39,750 --> 00:23:42,790
+ال salient اللى نعتقد او نعتقد ان هو distilled
+
+297
+00:23:42,790 --> 00:23:49,630
+water اذا واحد أبقال من المحلول اللى تركيزوا متين
+
+298
+00:23:50,040 --> 00:23:54,240
+طبعا هذا الواحد الأمقالي اللى كان تحكيزه 200 انا
+
+299
+00:23:54,240 --> 00:23:58,240
+دى حطيته وضبط عليه 9 أضعاف الكيمية من ال distill
+
+300
+00:23:58,240 --> 00:24:02,040
+water يعني حطيته واحد أمقاله وحطيت عليه 9 كمان من
+
+301
+00:24:02,040 --> 00:24:05,800
+ال distill water اذا فهى فيه تخفيف طبعا هى ما حدث
+
+302
+00:24:05,800 --> 00:24:10,000
+تخفيف كيف بي .. بي .. بي .. بيجي الأسئلة على هذا
+
+303
+00:24:10,000 --> 00:24:16,380
+النبط من النوع من ال dilution اول حاجة في قانون ال
+
+304
+00:24:16,380 --> 00:24:21,430
+dilution طبعاقانون الديوشن هو عبارة عن ال volume
+
+305
+00:24:21,430 --> 00:24:27,970
+of concentrated على
+
+306
+00:24:27,970 --> 00:24:30,310
+ال total أو ال final volume
+
+307
+00:24:34,450 --> 00:24:39,090
+طبعا و هذا طبعا القانون موجود معانا بالصلاحيات ده
+
+308
+00:24:39,090 --> 00:24:43,590
+و أنا جيت هنا حكى انه ال dilution represent the
+
+309
+00:24:43,590 --> 00:24:47,430
+ratio of concentrated of stick material of the
+
+310
+00:24:47,430 --> 00:24:52,550
+total final volume of solution يعني الناس معدل او
+
+311
+00:24:52,550 --> 00:24:56,990
+بالنسبة يعني قسمة not قسمة مين الحجم ال
+
+312
+00:24:56,990 --> 00:25:01,970
+concentrated لاش لال final volume على total volume
+
+313
+00:25:03,590 --> 00:25:08,370
+فهذا هو القانون
+
+314
+00:25:08,370 --> 00:25:10,270
+ال delution
+
+315
+00:25:13,280 --> 00:25:17,420
+قانون ال dilution هو ال volume of concentrated على
+
+316
+00:25:17,420 --> 00:25:20,960
+ال total volume ده خلينا نعمل مسألة جزئية هتحسب
+
+317
+00:25:20,960 --> 00:25:26,360
+إيش هو يعني ال dilution ال volume of concentrated
+
+318
+00:25:26,360 --> 00:25:30,420
+أنا كدش أخدت من ال stock من ال original تبعين من
+
+319
+00:25:30,420 --> 00:25:35,700
+ال مركز أخدت one أقل إذا بحط one أقل طيب ال total
+
+320
+00:25:35,700 --> 00:25:38,360
+volume كدش سعر في اليوتيوبة اللي مخفف فخرا عن ال
+
+321
+00:25:38,360 --> 00:25:43,270
+volume عندي واحد زائد تسعةطبعا هو يساوي واحد على
+
+322
+00:25:43,270 --> 00:25:50,010
+عشرة طبعا واحد على عشرة هذا هو ال .. ال .. ال ..
+
+323
+00:25:50,010 --> 00:25:53,490
+dilution تبع اليوبيا إذا هذه اليوبيا مخففتها عشر
+
+324
+00:25:53,490 --> 00:25:57,870
+مرات من ال .. ال original طبعا تكيزها عشر ال
+
+325
+00:25:57,870 --> 00:26:02,410
+original هي .. هي ال .. ال .. هي البحث بال
+
+326
+00:26:02,410 --> 00:26:11,640
+dilution طيب مثلالو كان عندي انا ال .. ال .. ال ..
+
+327
+00:26:11,640 --> 00:26:14,360
+المسألة هذه يقولي احسبي التركيز هنا، انا كده بدي
+
+328
+00:26:14,360 --> 00:26:19,080
+احسبها، أخدتوا قانون في التحليل يساوي M V يساوي M
+
+329
+00:26:19,080 --> 00:26:25,600
+شارطة V شارطة، هذا ما قبل التخليف، طبعا، و هذا بعد
+
+330
+00:26:25,600 --> 00:26:31,120
+التخليف كان، صح؟و .. او .. و موجود بصوتات نفس
+
+331
+00:26:31,120 --> 00:26:36,540
+القانون ولكن بس احنا معبرين عنه ب C V يساوي C V C1
+
+332
+00:26:36,540 --> 00:26:43,180
+V1 قبل التخييف و C2 V2 بعد التخييف طبعا؟ طيب C1
+
+333
+00:26:43,180 --> 00:26:49,580
+كام؟ كانت 200 بهذا المثال V1 كانت .. لنفرض ان ال B
+
+334
+00:26:49,580 --> 00:26:54,730
+مثلا هي عشر أقل حجمها هناطبعا .. بس هنا مابفيدني
+
+335
+00:26:54,730 --> 00:26:59,570
+.. انا مابفيدني جدش أخد 8 من ال .. منه .. أخد منه
+
+336
+00:26:59,570 --> 00:27:03,530
+واحد عشان أخففه .. يعني ضارب واحد ..ماشي ضارب واحد
+
+337
+00:27:03,530 --> 00:27:09,330
+هنا ..طبعا ال C2 جدش هو المجهول اللي مش معروفة ..
+
+338
+00:27:09,330 --> 00:27:15,060
+ال V2 طبعاال volume هنا إيجى تسعة عشرة في ال tube
+
+339
+00:27:15,060 --> 00:27:20,220
+هنا طبعا واحد تسعة عشرة فهذه المسألة عوّب فيها
+
+340
+00:27:20,220 --> 00:27:27,100
+فهنا مثلا على متين على عشرة هنا على عشرة و هنا على
+
+341
+00:27:27,100 --> 00:27:31,200
+عشرة بيروحوا حضر مع بعض و بيطلع ال concentration
+
+342
+00:27:31,200 --> 00:27:37,670
+C2 عشرينطبعا عشرين إيش؟ عشرين نفس التركيز
+
+343
+00:27:37,670 --> 00:27:42,410
+milligram بالأقل milligram بالأقل طبعا هذا هو
+
+344
+00:27:42,410 --> 00:27:50,470
+تركيز ال .. ال queue إذا لاحظته الأصل ميتين طبعا و
+
+345
+00:27:50,470 --> 00:27:55,110
+المخففة عشرين و المخففة فحكيتلكم إيش معناها عشر
+
+346
+00:27:55,110 --> 00:28:01,910
+الأصل عشر الميتين عشرين طبعافهي هنا ال .. يعني حلو
+
+347
+00:28:01,910 --> 00:28:06,090
+الربط ان طبت المعلومات بعضها البعض طيب كيف تيجي
+
+348
+00:28:06,090 --> 00:28:11,850
+الأسئلة أيضا على هذا الشكل؟ هي هنا مثلا عندي مثال
+
+349
+00:28:11,850 --> 00:28:15,530
+بالملزمة
+
+350
+00:28:15,530 --> 00:28:18,690
+أو بالمحاضرة تبعتنا هي
+
+351
+00:28:23,190 --> 00:28:28,170
+بدي أحضر 45 أمقال من محلول يكون تركيزه 30%
+
+352
+00:28:28,170 --> 00:28:33,150
+solution و ال stock تبعي من الأصل اللي هو 70%
+
+353
+00:28:33,150 --> 00:28:36,610
+تركيزه طبعا هذا كيف أنا بدي أطبقها يعني هلا بدي
+
+354
+00:28:36,610 --> 00:28:42,590
+أطبقهمهي هنا بدي احضر انا تلاتين في المية محلول و
+
+355
+00:28:42,590 --> 00:28:45,670
+ال final volume يكون خمسة و أربعين انا بدي خمسة و
+
+356
+00:28:45,670 --> 00:28:48,750
+أربعين من اقل من المحلول اللي تركيزه تلاتين في
+
+357
+00:28:48,750 --> 00:28:52,630
+المية من original عندي سبعين في المية طب قداش اخد
+
+358
+00:28:52,630 --> 00:28:58,410
+من ال original مش معروف بروح على C1 V1 يساوي C2 V2
+
+359
+00:28:58,410 --> 00:29:04,580
+والله بعد التخفيف تركيزي يكون تلاتينطبعا يا بنات
+
+360
+00:29:04,580 --> 00:29:08,980
+بدنا نوّه انه C1 يعني مقصود بيها concentration و
+
+361
+00:29:08,980 --> 00:29:14,780
+V1 volume one يعني قبل التخفيه ال initial و C2
+
+362
+00:29:14,780 --> 00:29:18,440
+طبعا concentration في volume بعد التخفيف عشان هي
+
+363
+00:29:18,440 --> 00:29:22,080
+اعطيناها الرقم اتنين نفس الفكرة M في V ال M
+
+364
+00:29:22,080 --> 00:29:26,400
+molarity يعني concentration ضرب V volume يساوي ال
+
+365
+00:29:26,400 --> 00:29:30,960
+molarity في ال volume يعني حاطيناهم شرطة يعني بعد
+
+366
+00:29:30,960 --> 00:29:34,440
+التخفيففهذه الفكرة بس عشان نفهم إيش يعني C وإيش
+
+367
+00:29:34,440 --> 00:29:37,460
+يعني V C concentration في volume يساوي ال
+
+368
+00:29:37,460 --> 00:29:41,140
+concentration في volume قبل التخفيف يساوي ما بعد
+
+369
+00:29:41,140 --> 00:29:45,820
+التخفيف هذا القانون أصلا المفروض هو موجود معاكم
+
+370
+00:29:45,820 --> 00:29:49,560
+بالتحليلية اللي هو عبارة عن number of moles يساوي
+
+371
+00:29:49,560 --> 00:29:53,240
+number of moles هذا تاخده في التحليلية مع المعلاقة
+
+372
+00:29:53,240 --> 00:29:56,920
+المهم انتوا تعرفوا ان C1 في V1 C يعني مقصود فيها
+
+373
+00:29:56,920 --> 00:30:02,550
+concentration و V1 مقصود فيها volumeC to
+
+374
+00:30:02,550 --> 00:30:06,130
+concentration و volume و لكن بعد التخفيف نفس فكرة
+
+375
+00:30:06,130 --> 00:30:10,650
+M و V ال M molarity يعني أصلا concentration احنا
+
+376
+00:30:10,650 --> 00:30:15,150
+من شوية لسه شرحنا حكينا ان ال molarity أحد طرق
+
+377
+00:30:15,150 --> 00:30:18,190
+التعبير عن ال concentration ال chemical unit تمام
+
+378
+00:30:18,190 --> 00:30:25,550
+فهي الفكرةو الحجم النهائي بتديها 45 و قبل التخفيف
+
+379
+00:30:25,550 --> 00:30:30,310
+انا عندي 70 فمش معروف عندى ال volume هذا جداش فعلى
+
+380
+00:30:30,310 --> 00:30:36,410
+طول تعويض مباشر تمام فبقسم و بحسب بيطلع معايا
+
+381
+00:30:36,410 --> 00:30:42,750
+الحجم زى ما هو موجود في ال slides 19.3 أمال تمام
+
+382
+00:30:42,750 --> 00:30:48,170
+هذا بيجي هيك طيب ممكن ييجي ايضا ييجي عندى مثلا
+
+383
+00:30:48,170 --> 00:30:54,990
+high etudeأخدنا منها حجم معين تمام بحيث أنه كانت
+
+384
+00:30:54,990 --> 00:30:59,290
+ال .. ال .. هنا تركيز خمسين في المية صار خمسة و
+
+385
+00:30:59,290 --> 00:31:04,890
+عشرين في المية تمام؟ و هنا الحجم أو ال volume
+
+386
+00:31:04,890 --> 00:31:11,370
+النهائي خمس أمقال تمام؟ مش معروف جدش أنا أخدت من
+
+387
+00:31:11,370 --> 00:31:11,630
+هنا
+
+388
+00:31:19,050 --> 00:31:24,010
+طبعا في هذا المثال معروفة ال C1 تركيز الأول هيو 50
+
+389
+00:31:24,010 --> 00:31:29,040
+% ال V1 أنا جدش أخدت هو اللي مجهولC2 خمسة و عشرين
+
+390
+00:31:29,040 --> 00:31:33,880
+في المية معروفة و V2 اللي هو ال final volume او ال
+
+391
+00:31:33,880 --> 00:31:38,560
+volume النهائي هي خمسة أمقال معروفة فيعني اذا V1
+
+392
+00:31:38,560 --> 00:31:41,220
+فقط هو اللي مجهول اللي هو ال volume اللي انا نقلته
+
+393
+00:31:41,220 --> 00:31:44,580
+عشان اعمله dilution تمام هو اللي مش موجود او مش
+
+394
+00:31:44,580 --> 00:31:51,180
+معروف دي التخيل جدش التركيز خمسة و عشرين تمام و
+
+395
+00:31:51,180 --> 00:31:57,790
+جدت او ال volume النهائي خمسة أمقال تماممش معروف
+
+396
+00:31:57,790 --> 00:32:03,950
+جداش انا اخدت من هنا طبعا فعلا طول بروح بحكي C1 V1
+
+397
+00:32:03,950 --> 00:32:09,810
+يساوي C2 V2 بعد التخفيف جداش التركيز خمسة و عشرين
+
+398
+00:32:09,810 --> 00:32:17,950
+طبعا و جداش ال volume النهائي خمسة طيب بس اضبط
+
+399
+00:32:17,950 --> 00:32:24,450
+الأوراق علشان تكون واضحة في ال .. طبعا اذا هي هنا
+
+400
+00:32:32,320 --> 00:32:38,120
+طبعا اذا ال بعد التخفيف كان 25 و ال volume كان 5
+
+401
+00:32:38,120 --> 00:32:41,560
+قبل التخفيف كان 50 بس انا مش عارفة كده اخدت من ال
+
+402
+00:32:41,560 --> 00:32:47,940
+original طبعا على طول بروح اش بعمل تعويض مباشر و
+
+403
+00:32:47,940 --> 00:32:53,840
+بطلع معايا الحجم اعتقد انه هيطلع اتنين و نص امقال
+
+404
+00:32:55,760 --> 00:33:00,720
+طبعا هذا بالنسبة لأ مسائل .. طبعا هذا إيش؟ هذا ال
+
+405
+00:33:00,720 --> 00:33:04,640
+V1 طبعا طلع عندي اتنين و نص
+
+406
+00:33:10,520 --> 00:33:16,580
+طيب هذا بالنسبة لأ ال .. ال .. ال .. الأسئلة طب
+
+407
+00:33:16,580 --> 00:33:19,940
+هانا .. هاي هذا النوع من الأسئلة إيش بحكيلك to ..
+
+408
+00:33:19,940 --> 00:33:23,760
+to make تمانية أمقال أنا بدي أعمل تمانية أمقال
+
+409
+00:33:23,760 --> 00:33:28,640
+يعني ال volume النهائي بدي يكون تمانية أمقالو 1 ل
+
+410
+00:33:28,640 --> 00:33:35,180
+20 dilution يعني انا التركيق بدي اعمل يعني مخفف
+
+411
+00:33:35,180 --> 00:33:41,440
+عشرين مرة يعني الاصل كان تركيزه عشرين و بدي اخففه
+
+412
+00:33:41,440 --> 00:33:44,620
+عشرين مرة عشان اصل له احد هيك معناه كلمة واحد
+
+413
+00:33:44,620 --> 00:33:48,100
+لعشرين خلينا نشوف ايش يعني كيف انا ممكن اطبق
+
+414
+00:33:48,100 --> 00:33:58,380
+باستخدام القانون C1 V1 يساوي C2 V2تمام ال C1 هذا
+
+415
+00:33:58,380 --> 00:34:04,380
+اش كان عندى التركيز الاول كان عشرين مركز عشرين مرة
+
+416
+00:34:04,380 --> 00:34:12,460
+و ال volume هذا مش معروفو ال C2 بدي اخففها مرة
+
+417
+00:34:12,460 --> 00:34:16,040
+واحدة انا بدي اخفف من عشرين لواحد مكتوب لواء one
+
+418
+00:34:16,040 --> 00:34:21,460
+in عشرين يعني من عشرين لواحد بدخليها تمام ال
+
+419
+00:34:21,460 --> 00:34:23,980
+concentration واحد في ال volume جديش ال volume
+
+420
+00:34:23,980 --> 00:34:27,980
+جالي تمانية امقال بدي احضر واحد اتعويض مباشر بجيب
+
+421
+00:34:27,980 --> 00:34:32,320
+بقسم على عشرين على عشرين بجيب هادي ال volume one
+
+422
+00:34:32,520 --> 00:34:37,980
+طبعا هايك بالنسبة لمن ل ال primary dilution كله
+
+423
+00:34:37,980 --> 00:34:43,980
+نفس الفكرة طيب و كيف بجيب ال dilution حكينا ان هو
+
+424
+00:34:43,980 --> 00:34:48,380
+عبارة عن ال ratio او النسبة ما بين ال volume ال
+
+425
+00:34:48,380 --> 00:34:52,500
+concentrated على ال total volume يعني مثلا نرجع
+
+426
+00:34:52,500 --> 00:34:56,600
+نضرب طبعا لما احكي primary dilution او simple
+
+427
+00:34:56,600 --> 00:35:00,660
+dilution نفس المعنى طبعا هو التخفيف اللى يجرى
+
+428
+00:35:00,660 --> 00:35:05,690
+بخطوة واحدةالاخر عشان نأكد الفكرة خلاص ونروح على
+
+429
+00:35:05,690 --> 00:35:09,790
+غيرها مثلا
+
+430
+00:35:09,790 --> 00:35:19,770
+هى في عندى tube تركيزها ثلاثة في المية تمام أخدت
+
+431
+00:35:19,770 --> 00:35:25,210
+منها واحد أو أخدت منها طبعا هى خليني أخدت خمسة
+
+432
+00:35:25,210 --> 00:35:29,570
+أمال في tube وكان في عندى فيها خمسة أمال
+
+433
+00:35:35,590 --> 00:35:41,750
+تمام فجدش ال concentration هنا بدى اروح او sorry
+
+434
+00:35:41,750 --> 00:35:45,150
+بلش قبل اسأل عن ال concentration جدش ال dilution
+
+435
+00:35:45,150 --> 00:35:48,590
+دلوقت يبقى هادى ال dilution قانون ال dilution هو
+
+436
+00:35:48,590 --> 00:35:52,850
+عبارة عن ايش نعمل سلايدات هو عبارة عن ال ratio لل
+
+437
+00:35:52,850 --> 00:35:59,630
+volume of concentrated صح على ال total volumeطيب
+
+438
+00:35:59,630 --> 00:36:04,090
+انا جدش أخدت من ال concentration؟ أخدت خمسة طبعا
+
+439
+00:36:04,090 --> 00:36:08,070
+على ال .. فوق ال total جدش؟ خمسة زائد خمسة عشرة
+
+440
+00:36:08,070 --> 00:36:13,710
+يعني خمسة على عشرة يعني نص طبعا هذا ال dilution نص
+
+441
+00:36:13,710 --> 00:36:16,590
+طب لو جالي احسب ال concentration عطول اش بروح
+
+442
+00:36:16,590 --> 00:36:25,540
+بعمله بحكيله c1 v1 فييساوي C2 V2 C1 جدش كان
+
+443
+00:36:25,540 --> 00:36:30,140
+تركيزها تلاتة في المية تلاتة طبعا ضرب V1 جدش أخدت
+
+444
+00:36:30,140 --> 00:36:34,320
+منها خمسة أمقال ويساوي C2 اللي مش معروفة و ال
+
+445
+00:36:34,320 --> 00:36:39,700
+volume النهائي كان عندي عشرة خمسة و خمسة عشرة طبعا
+
+446
+00:36:39,700 --> 00:36:48,780
+فإذا بعد هيك بطبق طاقة تطبيق مباشر للحسابات
+
+447
+00:36:48,780 --> 00:36:50,280
+و بجيب اللي هو C2
+
+448
+00:36:54,910 --> 00:36:58,090
+طبعا يا بنات انا لما ب .. اكتب القوانين بكتبها
+
+449
+00:36:58,090 --> 00:37:04,950
+مقتصرة لإنه مش مجدي بياخد وقت معايا و انا بحاول ان
+
+450
+00:37:04,950 --> 00:37:08,750
+انا اكتب ال .. القانون كامل ف volume of
+
+451
+00:37:08,750 --> 00:37:11,810
+concentrated اللي هي نفسها volume of sample أو
+
+452
+00:37:11,810 --> 00:37:15,310
+volume of concentrated solution عادي بس انا بختصر
+
+453
+00:37:15,310 --> 00:37:20,350
+في الكتابة ل .. لتوفير الوقت فقط لغبلة و انا بشرح
+
+454
+00:37:20,680 --> 00:37:24,280
+فهذا الاشي يعني خدوه بعين الاعتماد تركيزها تلاتة
+
+455
+00:37:24,280 --> 00:37:29,480
+في المية تلاتة سماء الاحسابات و بجيب اللي هو C2
+
+456
+00:37:29,480 --> 00:37:34,980
+كاتبلي في ال slides انه ال dilution عبارة عن ايه
+
+457
+00:37:34,980 --> 00:37:42,840
+عبارة عن ال volume of sample على ال total volume
+
+458
+00:37:42,840 --> 00:37:46,020
+طب يا miss انت حكيلي ان ال volume of concentrated
+
+459
+00:37:46,020 --> 00:37:49,340
+هنا طب ايش يعني ايش الفرق ما بين
+
+460
+00:37:53,600 --> 00:37:58,200
+طبعا الفرق ما بينهم طبعا مافيش فرق سواء انا حكيت
+
+461
+00:37:58,200 --> 00:38:01,480
+سواء كان عندي stock solution او concentrated
+
+462
+00:38:01,480 --> 00:38:05,140
+solution بدي اعمله تخفيف او سواء كان عندي sample
+
+463
+00:38:05,140 --> 00:38:10,070
+عين انا بدي اخففها و اشتغل عليها نفس المعنىيعني فى
+
+464
+00:38:10,070 --> 00:38:13,350
+النهاية اللى بيكون فى البسط هو ال volume اللى
+
+465
+00:38:13,350 --> 00:38:16,850
+أخدته من المادة المركزة أو المادة المراد تخفيفها
+
+466
+00:38:16,850 --> 00:38:20,850
+سواء كانت محلول أو عينة وعلى ال final volume جدش
+
+467
+00:38:20,850 --> 00:38:25,920
+صار فى الحجم النهائى بعد ما ضفت ال solvent عليهاهي
+
+468
+00:38:25,920 --> 00:38:29,860
+الفكرة او هيها ده معنى قانون ال delution تمام و
+
+469
+00:38:29,860 --> 00:38:34,160
+احنا ذكرنا سابقا في المحاضرة المسجلة انه انا
+
+470
+00:38:34,160 --> 00:38:38,200
+احيانا بضطر اخفف عينات لإنه التركيزها بيكون عالي و
+
+471
+00:38:38,200 --> 00:38:40,920
+الأجهزة اللي عندي و المواد اللي عندي قدرة
+
+472
+00:38:40,920 --> 00:38:43,880
+استيعابها مابتقدرش تجيس للتركيز اللي موجودة في
+
+473
+00:38:43,880 --> 00:38:47,460
+العينة وضربتلكم مثال لما يكون السكر تركيزه 500
+
+474
+00:38:47,460 --> 00:38:54,500
+وعندي محلول بيجيس يعنيلأ انكترت يعني ان لأ يعني
+
+475
+00:38:54,500 --> 00:38:57,420
+على أقصى استيعاب عنده بي .. بيجيسل لحد تلاتمية
+
+476
+00:38:57,420 --> 00:39:00,960
+فإيش أعمل أنا بهاي الحالة؟ بخفف العينة طب كيف بدي
+
+477
+00:39:00,960 --> 00:39:04,800
+أخفف العينة؟ بدي أستخ .. أاخد منها حجم و أضيفه على
+
+478
+00:39:04,800 --> 00:39:08,300
+ال sample فأضيف حجم من ال sample و أضيف عليه
+
+479
+00:39:08,300 --> 00:39:11,410
+solventطبعا كيف بدى أحسب ال dilution للتخفيف
+
+480
+00:39:11,410 --> 00:39:14,830
+التخفيف اللى حصل هنا للعينة جدش أنا أخدت من ال
+
+481
+00:39:14,830 --> 00:39:18,750
+sample على حجم ال sample مع ال solvent كلهم سوا ال
+
+482
+00:39:18,750 --> 00:39:23,150
+total هيك بكون طلعت ال dilution تمام الجزء الآخر
+
+483
+00:39:23,150 --> 00:39:30,090
+من المحاضرة لحظة هى الجزء الآخر انا طبعا مصوراهم ف
+
+484
+00:39:30,090 --> 00:39:36,530
+..هى اللى احنا بنكون مركزينها يعنى تم فصلهم لجزئين
+
+485
+00:39:36,530 --> 00:39:39,450
+يعنى ما بعرف ليش انفصلوا او فصل التسجيل اثناء
+
+486
+00:39:39,450 --> 00:39:43,710
+تصويرى ما علينا هذا هو الجزء الاخر نستكمل من عند
+
+487
+00:39:43,710 --> 00:39:49,310
+القانون ال delution القانون التانى لش كان تتملناه
+
+488
+00:39:49,310 --> 00:39:52,230
+فى ال slides ان هو volume of sample على ال total
+
+489
+00:39:52,230 --> 00:39:55,450
+volume هى هنا شرح و إعادة لنفس الكلام اللى ذكرته
+
+490
+00:39:55,450 --> 00:40:00,690
+من شويةهي اللي احنا بنكون مركزين بتكون بدأ أخففها
+
+491
+00:40:00,690 --> 00:40:03,870
+أو بتكون مركزة هي نفس الفكرة يعني volume of sample
+
+492
+00:40:03,870 --> 00:40:06,950
+او volume concentrated نفس الاشياء على ال total
+
+493
+00:40:06,950 --> 00:40:11,210
+volumeطيب لو انا بدي اجيب هذه ال cube ال dilution
+
+494
+00:40:11,210 --> 00:40:15,170
+تبعها نص لو بدي اجيب حالة اسمها dilution factor
+
+495
+00:40:15,170 --> 00:40:18,650
+ايش يعني يعني مقلوب النص مقلوب ال dilution ال
+
+496
+00:40:18,650 --> 00:40:22,830
+dilution factor هو عبارة عن واحد على ال dilution و
+
+497
+00:40:22,830 --> 00:40:33,130
+يساوي واحد على ال واحد على النص يعني اتنين مقلوب
+
+498
+00:40:33,130 --> 00:40:37,700
+ال dilution يعني اتنينتمام هذا هو ال dilution
+
+499
+00:40:37,700 --> 00:40:40,480
+factor بنزمني كمان شوية لما احكي عن ال cereal
+
+500
+00:40:40,480 --> 00:40:49,940
+dilution طيب نيجي نحكي عن ال cereal dilutions كيف
+
+501
+00:40:49,940 --> 00:40:54,020
+انا بستخدم .. ب .. بعمل ال cereal dilution تمام ال
+
+502
+00:40:54,020 --> 00:40:56,680
+cereal dilution حاكية انه مثلا لو كان عندى تيوب
+
+503
+00:40:56,680 --> 00:41:05,440
+تركيزين تمام molar ومحلول مثلا ال glucoseطمام؟ و
+
+504
+00:41:05,440 --> 00:41:10,920
+أنا أخدت منه one ml و حطيتها في تيوب فيها تسعة ml
+
+505
+00:41:10,920 --> 00:41:14,720
+distilled water بعدين أخدت في تيوب تانية one ml و
+
+506
+00:41:14,720 --> 00:41:18,240
+حطيتها في تيوب فيها تسعة ml من ال distilled water
+
+507
+00:41:18,240 --> 00:41:21,960
+و أخدت في .. في تيوب خمسة ml و حطيتها في تيوب كان
+
+508
+00:41:21,960 --> 00:41:26,190
+فيها خمسة ml من ال distilled waterانا عندها الـ
+
+509
+00:41:26,190 --> 00:41:29,790
+get a tube رقم واحد اتنين تلاتة تلت قنابيب انا
+
+510
+00:41:29,790 --> 00:41:33,570
+عاملة لهم تخطيط طيب لو انا بدي اجي اعمل حسابات
+
+511
+00:41:33,570 --> 00:41:37,510
+لهاي التلت قنابيب اول حاجة بدي احسب ال dilution
+
+512
+00:41:37,510 --> 00:41:41,030
+لتلت قنابيب هو عبارة عن ال volume ل ال
+
+513
+00:41:41,030 --> 00:41:46,430
+concentrated على ال total volume صح؟ طيب ال .. كده
+
+514
+00:41:46,430 --> 00:41:50,490
+اش انا خدت من ال total واحد هي واحد على ال total
+
+515
+00:41:50,490 --> 00:41:56,430
+واحد زائد تسعة يساوي عشر عشرطيب ال tube هذه انا
+
+516
+00:41:56,430 --> 00:41:59,350
+جدش اخدت من ال .. من اللي جا .. من ال concentrated
+
+517
+00:41:59,350 --> 00:42:02,830
+يعني من اللي جابلها المركزة و اكتر منها اخدت واحد
+
+518
+00:42:02,830 --> 00:42:08,810
+طبعا على واحد زائد تسعة عشرة بنفع اقول ال tube هذه
+
+519
+00:42:08,810 --> 00:42:15,000
+تخفيفها عشر و سقط لأهذه تخفيفها عشر من الأصل لكن
+
+520
+00:42:15,000 --> 00:42:18,320
+هذه تخفيفها عشر من ال .. من التيوبة اللي جابلها
+
+521
+00:42:18,320 --> 00:42:23,420
+اللي ألضم تخفيفها عشر يعني ان التيوبة رقم واحد صح
+
+522
+00:42:23,420 --> 00:42:27,520
+تخفيفها طولها عشر، واحد على عشرة بس هي من الأصل
+
+523
+00:42:27,520 --> 00:42:32,430
+عشرفواحدة .. لو أنا جيت حكيت dilution of تيوب one
+
+524
+00:42:32,430 --> 00:42:37,490
+واحد على عشرة و ساكتت، عادي، تمام؟ فلإنه .. لإنها
+
+525
+00:42:37,490 --> 00:42:41,430
+جاية من الأصل لكن هاي ما بنفع أحكي إن هاي التيوب
+
+526
+00:42:41,430 --> 00:42:45,570
+تخفيفها أيضا واحد لعشرة، لأ هي تخفيفها واحد لعشرة
+
+527
+00:42:45,570 --> 00:42:50,160
+من التيوب اللي سابقاهاتمام؟ فما بنفع أحكي انه
+
+528
+00:42:50,160 --> 00:42:53,620
+تخفيفها واحد لعشرة واسكت لأ بدي أكمل بدي أحكي واحد
+
+529
+00:42:53,620 --> 00:42:57,980
+لعشرة من مين؟ من التيوب اللي سبقتها اللي أصلا أيضا
+
+530
+00:42:57,980 --> 00:43:02,000
+تخفيفها واحد لعشرة فأصبح بيصير تخفيف التيوب رقم
+
+531
+00:43:02,000 --> 00:43:05,620
+اتنين واحد لعشرة من التيوب رقم واحد اللي تخفيفها
+
+532
+00:43:05,620 --> 00:43:09,500
+ايضا واحد لعشرة طب كيف ده اختصر هذا الكلام كله؟
+
+533
+00:43:09,500 --> 00:43:13,660
+بدي اقول واحد لعشرة درب واحد لعشرة اللي هنا ويسوي
+
+534
+00:43:13,660 --> 00:43:19,400
+واحد على مية تخفيفهاهي معاكم الشريح حلو بتشوفوا
+
+535
+00:43:19,400 --> 00:43:22,080
+كده، اذا انا لو بدي اجيب ال dilution اللي هي ده
+
+536
+00:43:22,080 --> 00:43:27,740
+المفروض اقول عشر اللي الها جاي من العشر اللي
+
+537
+00:43:27,740 --> 00:43:33,190
+جابلها يعني عشر في عشر و يسوي واحد عالميامضربهم في
+
+538
+00:43:33,190 --> 00:43:38,730
+بعض طيب نفس الفكرة هنا جدش ال delution أخدت خمسة
+
+539
+00:43:38,730 --> 00:43:42,550
+من المركز اللي جابلها أو الأكتر تركيزا منها خمسة
+
+540
+00:43:42,550 --> 00:43:46,950
+على إيش خمسة زائد خمسة عشرة يعني إيش نص مابنفع
+
+541
+00:43:46,950 --> 00:43:53,390
+أقول نص و أسكت لأ نص من المخففة نص من اللي جابلها
+
+542
+00:43:54,070 --> 00:43:59,210
+طبعا لو أنا قلت نص و أسكت معناها أنها مخففة نص من
+
+543
+00:43:59,210 --> 00:44:02,950
+ال original و هذا غلط لأ هي مخففة نص من اللي قبلها
+
+544
+00:44:02,950 --> 00:44:07,610
+يعني نص ضرب اللي قبلها جداش واحدة عالمية يسوى واحد
+
+545
+00:44:07,610 --> 00:44:08,630
+على تان
+
+546
+00:44:10,980 --> 00:44:13,920
+ال .. ال .. ال dilution اذا هذه تخطيطها واحد لعشرة
+
+547
+00:44:13,920 --> 00:44:17,700
+تيوب رقم واحد تيوب رقم اتنين واحد لمية تيوب رقم
+
+548
+00:44:17,700 --> 00:44:22,600
+تلاتة واحد لمتان طيب لو انا بدى اجيب التركيز انا
+
+549
+00:44:22,600 --> 00:44:26,220
+.. انا عارفة ان ال original تركيزها مية طيب جداش
+
+550
+00:44:26,220 --> 00:44:28,880
+بدى اجيب .. لو بدى اجيب التركيز عفوا ان ال
+
+551
+00:44:28,880 --> 00:44:31,840
+original تركيزها متين هلأ انا بدى اجيب تركيز
+
+552
+00:44:31,840 --> 00:44:36,900
+الانبيب كيف بدى اشتغل التركيز لكل تيوب اش بدى اعمل
+
+553
+00:44:36,900 --> 00:44:43,370
+التركيز ل .. of تيوبconcentration of a tube تركيز
+
+554
+00:44:43,370 --> 00:44:50,210
+الأنابيب اللى عندى تساوي ال dilution of a tube
+
+555
+00:44:50,210 --> 00:44:56,390
+تمام ضرب ال original concentration
+
+556
+00:44:58,990 --> 00:45:02,870
+طيب اش يعني هاي ال tube رقم واحد؟ ال tube رقم واحد
+
+557
+00:45:02,870 --> 00:45:05,510
+ال dilution of ال tube يعني ال dilution of ال tube
+
+558
+00:45:05,510 --> 00:45:11,350
+رقم واحد جداش بيطلع واحد على عشرة طبعا ضرب ال
+
+559
+00:45:11,350 --> 00:45:14,190
+original concentration جداش ال original
+
+560
+00:45:14,190 --> 00:45:18,150
+concentration 200 إذا بضرب في 200 و بده يطلع ليه
+
+561
+00:45:18,150 --> 00:45:27,460
+عشرين molar طيب هاي تخفيفهاواحر عالمية، إذا الـ
+
+562
+00:45:27,460 --> 00:45:31,660
+dilution تبعها واحر عالمية ضارب 200 ويساوي 2 molar
+
+563
+00:45:31,660 --> 00:45:38,140
+طب هالي تخفيكها واحر على 200 في 200 تركيز ال
+
+564
+00:45:38,140 --> 00:45:43,220
+original concentration ويساوي 1 molar إذا أنا بقدر
+
+565
+00:45:43,220 --> 00:45:46,440
+من خلال هذا القانون ال concentration of a tube
+
+566
+00:45:46,440 --> 00:45:49,440
+أجيب تركيز القنابيب التلاتة اللي حصلت عليهم في ال
+
+567
+00:45:49,440 --> 00:45:55,560
+cereal dilutionطيب على فرض .. على فرض أنه هذه
+
+568
+00:45:55,560 --> 00:45:59,920
+القيم .. هذه القيم أعطانيها .. هو أعطانيها
+
+569
+00:46:03,010 --> 00:46:09,390
+ولكن ولكن ان هو انه ال original اللى كان مجهول مش
+
+570
+00:46:09,390 --> 00:46:12,190
+معروف انا مش عارفة ال original هذا جداش بدى
+
+571
+00:46:12,190 --> 00:46:16,090
+بالاكاد بدى احسبه خلينى نشوف ما هو يا اما بيعطينى
+
+572
+00:46:16,090 --> 00:46:19,010
+ال original و بقولى احسب ال tube ال concentration
+
+573
+00:46:19,010 --> 00:46:21,830
+of ال tube يا اما بيعطينى ال concentration of ال
+
+574
+00:46:21,830 --> 00:46:25,110
+tube و بقولى احسب ال original هلأ انا افترض انه
+
+575
+00:46:25,110 --> 00:46:28,610
+معاكي ال concentration of ال tube و بدى تحسبيه ال
+
+576
+00:46:28,610 --> 00:46:34,750
+original هى ال originalconcentration يساوي ال
+
+577
+00:46:34,750 --> 00:46:42,170
+dilution factor of ال cube ضرب ضرب تمام ال
+
+578
+00:46:42,170 --> 00:46:49,970
+concentration of the same cube اش يعني خليني نطبق
+
+579
+00:46:49,970 --> 00:46:53,050
+ال cube رقم واحد ال cube رقم واحد قداش ال dilution
+
+580
+00:46:53,050 --> 00:46:56,700
+factorال dilution factor احنا حكينا مطلوب ال
+
+581
+00:46:56,700 --> 00:47:01,020
+dilution يعني هنا ال dilution واحد على عشرة معناها
+
+582
+00:47:01,020 --> 00:47:05,980
+ال dilution factor يساوي عشرة تمام؟ يساوي عشرة هنا
+
+583
+00:47:05,980 --> 00:47:12,840
+ال dilution factor فإذا ال dilution factor عشرةالـ
+
+584
+00:47:12,840 --> 00:47:15,620
+Concentration of the same tube قدش ميعطينياها
+
+585
+00:47:15,620 --> 00:47:19,600
+نفترض انه عطانياها عشرين عشرة في عشرين ويساوي متين
+
+586
+00:47:19,600 --> 00:47:25,040
+تمام حساباتنا صح طيب خلينا نطبق في ال tube رقم
+
+587
+00:47:25,040 --> 00:47:28,580
+اتنين ال tube رقم اتنين هنا ال dilution factor
+
+588
+00:47:28,580 --> 00:47:32,640
+مقلوب ال dilution قداش مية واحدة عالمية مقلوبها
+
+589
+00:47:32,640 --> 00:47:37,480
+مية مية في اتنين200 الـ dilution factor لها دي 200
+
+590
+00:47:37,480 --> 00:47:42,780
+مقلوبها واحد على 200 طبعا هي ال dilution ال
+
+591
+00:47:42,780 --> 00:47:46,520
+dilution factor 200 200 في واحد 200 اذا ال
+
+592
+00:47:46,520 --> 00:47:49,880
+original بقدر احسبه ان هو عبارة عن ال dilution
+
+593
+00:47:49,880 --> 00:47:53,340
+factor لليتيوب ضرب التركيز نفس ليتيوب مابنفع اقول
+
+594
+00:47:53,340 --> 00:47:56,000
+والله ال dilution factor لها دي ليتيوب عشرة وروح
+
+595
+00:47:56,000 --> 00:48:00,350
+اضربه في تركيز ليتيوب رقم اتنين غلططبعا لازم انه
+
+596
+00:48:00,350 --> 00:48:04,890
+اضربه في تركيز نفس ال cube اللي هو عشرة في عشرين
+
+597
+00:48:04,890 --> 00:48:12,070
+مية في اتنين متين في واحد و هكذا طبعا طيب نضرب
+
+598
+00:48:12,070 --> 00:48:18,310
+منها الاخر و ننهي فيه علشان يكون معنى واضح كل إشي
+
+599
+00:48:18,310 --> 00:48:22,190
+أخدت عن ال cube طبعا .. طبعا هذه ال stock او قولنا
+
+600
+00:48:22,190 --> 00:48:30,360
+ال original طبعاتركيزها مثلا خمسمية ميلي اجرام
+
+601
+00:48:30,360 --> 00:48:36,380
+بالديسيلاتر أخدت منها اش حاكينا واحد امقلتمام و
+
+602
+00:48:36,380 --> 00:48:40,240
+عندي تسعة أمقال distilled water و أخدت كمان أيضا
+
+603
+00:48:40,240 --> 00:48:45,080
+واحد أمقال و عندي تسعة أمقال distilled water تمام
+
+604
+00:48:45,080 --> 00:48:49,760
+و .. او خليني أحكي مثلا هنا أخدت اتنين أمقال و في
+
+605
+00:48:49,760 --> 00:48:54,200
+عندي تمانية أمقال distilled water و هنا مثلا أخدت
+
+606
+00:48:54,200 --> 00:49:00,940
+خمسة أمقال و في عندي خمسة أمقال distilled water
+
+607
+00:49:01,360 --> 00:49:05,480
+طبعا يلّا نحسب ال dilution للقنابيب التلاتة هي
+
+608
+00:49:05,480 --> 00:49:09,640
+عندي انبوبة رقم واحد انبوبة رقم اتنين انبوبة رقم
+
+609
+00:49:09,640 --> 00:49:14,380
+تلاتة ال dilution قولنا ال volume of concentrated
+
+610
+00:49:14,380 --> 00:49:18,300
+على ال total volume ال concentrated على ال total
+
+611
+00:49:18,300 --> 00:49:24,080
+volume طبعا واحد لل tube رقم واحد واحد على واحد
+
+612
+00:49:24,080 --> 00:49:28,820
+زائد تسعة يعني واحد على عشرةاتنين على اتنين زائد
+
+613
+00:49:28,820 --> 00:49:33,460
+تمانية اتنين على اتنين زائد تمانية ويساوي اتنين
+
+614
+00:49:33,460 --> 00:49:37,800
+على عشرة قولنا بنسكت هنا لأ اتنين على عشرة من مين
+
+615
+00:49:37,800 --> 00:49:43,420
+من اللي جابلها تمام فبتصير اتنين على عشرة ضرب واحد
+
+616
+00:49:43,420 --> 00:49:49,620
+على عشرة ويساوي اتنين على مية تمام يعني مثلا واحد
+
+617
+00:49:49,620 --> 00:49:56,250
+على خمسين ال daily oceanطيب خلينا نشوف ال tube رقم
+
+618
+00:49:56,250 --> 00:50:00,410
+تلاتة جديش كان ال ball اللي أخدناها من اللي جابلها
+
+619
+00:50:00,410 --> 00:50:06,450
+خمسة تمام على خمسة زائد خمسة ال total عشرة يعني نص
+
+620
+00:50:06,450 --> 00:50:11,870
+نص واسكت لأ نص في اللي .. نص من اللي جابلها يعني
+
+621
+00:50:11,870 --> 00:50:19,520
+نص ضرب واحد على خمسين ويساوي واحد على مية تمامهذا
+
+622
+00:50:19,520 --> 00:50:23,260
+اللي هي dilution طيب ال dilution factor خلينا
+
+623
+00:50:23,260 --> 00:50:28,440
+نحسبه بالمرة هذه عشرة هذه خمسين هذه مية المقلوب
+
+624
+00:50:28,440 --> 00:50:32,000
+طبعا اللي هو عبارة عن إيش واحدة على ال dilution
+
+625
+00:50:32,000 --> 00:50:36,880
+طبعا طيب
+
+626
+00:50:36,880 --> 00:50:43,540
+ماشي إذا هحسبنا ال dilution طب ال concentration of
+
+627
+00:50:43,540 --> 00:50:49,700
+tubesتمام ال concentration of tubes اللي هو حكينا
+
+628
+00:50:49,700 --> 00:50:54,340
+عبارة عن ال dilution ضرب ال original واحدة على
+
+629
+00:50:54,340 --> 00:51:00,560
+عشرة في خمسمية تمام خمسين واحدة على خمسين في
+
+630
+00:51:00,560 --> 00:51:10,790
+خمسمية عشرة تمام واحدة على مية في خمسمية خمسةهي
+
+631
+00:51:10,790 --> 00:51:15,450
+التركيز طبعا اش نفس الوحدة بياخده milligram per
+
+632
+00:51:15,450 --> 00:51:20,810
+deciliter طبعا نفس وحدة الاصل طيب على فرض حكينا
+
+633
+00:51:20,810 --> 00:51:26,010
+انه مثلا نفترض جدلا انه هذه ماعطانيها طبعا
+
+634
+00:51:26,010 --> 00:51:29,770
+ماعطانيها و ماعطيني هاي التركيز هادي بدي احسب ال
+
+635
+00:51:29,770 --> 00:51:36,490
+concentration ال concentration oforiginal اش بدو
+
+636
+00:51:36,490 --> 00:51:41,390
+يكون؟ بدو يكون ال dilution factor في ال .. ال ..
+
+637
+00:51:41,390 --> 00:51:45,010
+ال concentration للي تيوب صح؟ ال concentration للي
+
+638
+00:51:45,010 --> 00:51:51,410
+تيوب طيب يعني عشرة في خمسين هاي ال dilution factor
+
+639
+00:51:51,410 --> 00:51:53,710
+و هاي ال concentration للي تيوب عشرة في خمسين
+
+640
+00:51:53,710 --> 00:51:58,570
+خمسمية اذا شغل صح خمسة في عشر .. خمسين في عشرة
+
+641
+00:51:58,570 --> 00:52:04,520
+خمسمية مية في خمسةخمسمية تمام ممكن هذه ما اعطيكي
+
+642
+00:52:04,520 --> 00:52:07,360
+اياها انا و احكيلك .. و اعطيكي تركيز واحدة من
+
+643
+00:52:07,360 --> 00:52:12,620
+القنابيب التانية و اقولك احسبي يالا ممكن مثلا اكون
+
+644
+00:52:12,620 --> 00:52:18,120
+اعطياكي تركيز تيوبة واحدة و اقولك عادي كملي احسب
+
+645
+00:52:18,120 --> 00:52:21,960
+تركيز القنابيب التانية و احسب الاصل سهل جدا بقراص
+
+646
+00:52:21,960 --> 00:52:26,300
+لو انك انت تتبعي القوانين مهم جدا تعرفي ايش قانون
+
+647
+00:52:26,300 --> 00:52:30,090
+ال delusionأيش قانون ال dilution factor؟ أيش قانون
+
+648
+00:52:30,090 --> 00:52:33,110
+ال concentration of the tube؟ اللي احنا كتبناه
+
+649
+00:52:33,110 --> 00:52:37,070
+وقولنا عبارة عن ال dilution في ال original
+
+650
+00:52:37,070 --> 00:52:40,890
+concentration تمام؟ وقانون ال original
+
+651
+00:52:40,890 --> 00:52:43,290
+concentration اللي هو عبارة عن ال dilution factor
+
+652
+00:52:43,290 --> 00:52:47,340
+of الدرب ال concentration of the semi tubeهدول مهم
+
+653
+00:52:47,340 --> 00:52:52,100
+جدا انو تعرفوها حاولوا انو اتدربوا ايداكوا على ال
+
+654
+00:52:52,100 --> 00:52:55,600
+.. الأسئلة اللي موجودة في الملزمة في نفس ال
+
+655
+00:52:55,600 --> 00:52:59,000
+chapter علشان انو تشوفوا حالكم كيف انتوا بقى مع
+
+656
+00:52:59,000 --> 00:53:04,380
+الشرح هل استفدتوا او لأ و انا ممكن ابقى قاعد هيك
+
+657
+00:53:04,380 --> 00:53:08,020
+برضه ابعتلكم أسئلة و احكيلكم جربوا تحلوها تمام
+
+658
+00:53:08,020 --> 00:53:12,780
+فسهل جدا بس تطبيق قوانين و حسابات يعني بس اطمشتي
+
+659
+00:53:12,780 --> 00:53:20,330
+ال .. ذاكرتك الحسابيةبالنسبة للحسابات
+
+660
+00:53:20,330 --> 00:53:24,450
+و التخفيفات طبعا هذا ايش نحكي عنه serial dilution
+
+661
+00:53:24,450 --> 00:53:27,490
+لإنه أنا بمشي بشري .. بشري طريقة بعمل multiple
+
+662
+00:53:27,490 --> 00:53:32,050
+dilution و بمشي بطريقة متسلسلة و ال .. ال .. ال ..
+
+663
+00:53:32,050 --> 00:53:35,190
+التخفيك هذا بيكون من الأصل و هذه .. و التخفيف تبع
+
+664
+00:53:35,190 --> 00:53:37,590
+الكيوب التانية بيكون من الأولى و التارتة من
+
+665
+00:53:37,590 --> 00:53:42,520
+التانية و هكذاعشان هيك بسميه serial متسلسل تمام
+
+666
+00:53:42,520 --> 00:53:47,740
+نيجي لسلايدات اخر slide مثلا هي هنا بيعطيني مثال
+
+667
+00:53:47,740 --> 00:53:48,900
+هنا
+
+668
+00:53:59,300 --> 00:54:07,700
+عينة بكتيريا اخد منها واحد امقال وحطها على تسعة
+
+669
+00:54:07,700 --> 00:54:10,860
+امقال يعني ال total صار عشرة يعني ال dilution اللى
+
+670
+00:54:10,860 --> 00:54:15,560
+هدى اش واحد على عشرة تمام طيب اخد منها واحد امقال
+
+671
+00:54:15,560 --> 00:54:18,640
+وكان في عندى تسعة امقال distilled water يعني برضه
+
+672
+00:54:18,640 --> 00:54:22,540
+واحد على واحد زائد تسعة يعني واحد على عشرة من مين؟
+
+673
+00:54:22,760 --> 00:54:27,300
+من هذه يعني واحد على عشر ضرب واحد على عشر يعني
+
+674
+00:54:27,300 --> 00:54:31,420
+واحد على مية تمام طيب أخدنا من الواحد على مية هذه
+
+675
+00:54:31,420 --> 00:54:35,880
+one ml وحطيتها على تسعة ml distilled water فصار
+
+676
+00:54:35,880 --> 00:54:39,660
+عندى تخفيفها واحد على عشرة من مين من الواحد على
+
+677
+00:54:39,660 --> 00:54:43,280
+مية يعني واحد على عشر ضرب واحد على مية يعني واحد
+
+678
+00:54:43,280 --> 00:54:48,280
+على الف اذا التخفيف ال dilution اشماله واحد على
+
+679
+00:54:48,280 --> 00:54:52,230
+الف يعني عشر أسالت تلاتةو ال dilution factor ألف
+
+680
+00:54:52,230 --> 00:54:56,690
+طبعا هنا في نقطة مهمة لازم احنا نوح لها انه لما
+
+681
+00:54:56,690 --> 00:55:02,010
+اجي احكيلك احسري ال final dilution ايش يعني final
+
+682
+00:55:02,010 --> 00:55:06,330
+dilution مكتوب بالسلايدات انه ال final dilution هو
+
+683
+00:55:06,330 --> 00:55:13,310
+حاصل ضرب تخفيفاتهم التلاتة او هو تخفيف البوبة
+
+684
+00:55:13,310 --> 00:55:19,800
+الأخيرة يعني مثلا هذه كانت واحد على تسعة عشرو هاد
+
+685
+00:55:19,800 --> 00:55:25,560
+اتنين على تمانية اتنقل هي كانت يعني اتنين على عشرة
+
+686
+00:55:25,560 --> 00:55:31,140
+يعني خمس و هاد كانت نص تمام لو جيت ضربت من نص
+
+687
+00:55:31,140 --> 00:55:34,960
+بالاتنين على عشرة بالواحد على عشرة بتطلع عندى اللى
+
+688
+00:55:34,960 --> 00:55:38,040
+هو تركيز التخليف الانبوبة الأخيرة اللى هو واحدة
+
+689
+00:55:38,040 --> 00:55:41,440
+على ميةطبعا نفس الشغل اللى كنا نشتغله مش انا كنت
+
+690
+00:55:41,440 --> 00:55:44,460
+هانا طلعتها واحد على عشرة بعدين هذه قلت اتنين على
+
+691
+00:55:44,460 --> 00:55:50,020
+عشرة يعني خمس صح هذه خمسة خمس طبعا خمس في الواحد
+
+692
+00:55:50,020 --> 00:55:55,320
+على عشرة و هانا طلعت انه ال ..وطلع عندى واحدة على
+
+693
+00:55:55,320 --> 00:55:59,240
+خمسين وانا طلعتها انها نص وضربتها بالواحدة على
+
+694
+00:55:59,240 --> 00:56:02,840
+خمسين يعني سواء طلعت وقولت هذه عشر وهذه اتنين على
+
+695
+00:56:02,840 --> 00:56:06,620
+عشرة وهذه نص وضربتهم في بعض او اشتغلت الشغل هذا
+
+696
+00:56:06,620 --> 00:56:12,020
+لإنه انا طلع كل dilution لحال نفس الاشي المهم انه
+
+697
+00:56:12,020 --> 00:56:15,800
+انا ال final dilution هو تخفيك للانبوبة الأخيرة او
+
+698
+00:56:15,800 --> 00:56:19,920
+حاصل ده التخفيك اللى انا بيدل حالها تمام هذا
+
+699
+00:56:19,920 --> 00:56:26,880
+بالنسبة لالـ Final Dilution لما أجي أطلب أحكي الـ
+
+700
+00:56:26,880 --> 00:56:29,660
+Final Dilution عادي هي بتعطيني تخفيق الأنبوبة
+
+701
+00:56:29,660 --> 00:56:34,060
+الأخيرة يا إما بتقولي هذه واحدة على عشرة وسكتنا
+
+702
+00:56:34,060 --> 00:56:39,440
+وهذه اتنين على عشرة وسكتنا وهذه نص وسكتنا وبعدين
+
+703
+00:56:39,440 --> 00:56:44,520
+ضربناهم في بعضطبعا او بنقول واحد على عشرة و بعدين
+
+704
+00:56:44,520 --> 00:56:47,720
+بنقول هذه اتنين على عشرة و مانسكتش و مانضربها في
+
+705
+00:56:47,720 --> 00:56:52,100
+الواحد على عشرة اللي قبلها بعدين نقول ان هذه نص و
+
+706
+00:56:52,100 --> 00:56:54,660
+بعدين مانسكتش و مضربها في الواحد على خمسين اللي
+
+707
+00:56:54,660 --> 00:56:58,660
+قبلها و تطلع لي واحد على مية و اقول ان هذا هو ال
+
+708
+00:56:58,660 --> 00:57:02,280
+final conclusion سواء اشتغلت هيك او هيك نفس الفكرة
+
+709
+00:57:02,280 --> 00:57:11,650
+طبعا اذاهيك بنكون خلصنا المحاضرة تبع ال solution
+
+710
+00:57:11,650 --> 00:57:14,730
+and dilution و أتمنى أنه يكون الاشي هذا ساعدكم
+
+711
+00:57:14,730 --> 00:57:19,570
+بالفهم و طريقة الشرح على الورق قربت الاشي لأذهانكم
+
+712
+00:57:19,570 --> 00:57:25,170
+و خلت الوضع يعني يمكن تخيله أو يمكن إدراكه و
+
+713
+00:57:25,170 --> 00:57:26,030
+يعطيكم العافية
+
+714
+00:57:30,330 --> 00:57:34,410
+طيب يا صبايا هلأ المطلوب منكم أنه المسائل اللي
+
+715
+00:57:34,410 --> 00:57:39,330
+موجودة بالمحاضرة تمام ترجعوا تحلوها و تشوفوا
+
+716
+00:57:39,330 --> 00:57:44,470
+تستفيدوا من الأفكار اللي موجودة فيها تمام و تشوفي
+
+717
+00:57:44,470 --> 00:57:47,790
+حالك أنت هلأ فاهمة أو لأ اتعاملي مع السؤال زي كأنه
+
+718
+00:57:47,790 --> 00:57:50,970
+الإجابة مش موجودة في الملزمة أو في ال .. في ال
+
+719
+00:57:50,970 --> 00:57:54,170
+power point و حللي لحالك قايمي نفسك شوفي حالك
+
+720
+00:57:54,170 --> 00:57:59,200
+فاهمة أو لأ علشانمحاضرة المناقشة على ال google
+
+721
+00:57:59,200 --> 00:58:02,960
+meet يوم السبت يكون فيها انتوا تناقشوني بالاشياء
+
+722
+00:58:02,960 --> 00:58:05,420
+اللي انتوا مش فاهمينها او اللي مش قادرين تفهموها
+
+723
+00:58:05,420 --> 00:58:09,640
+طبعا عشان اقدر اجاوبكم عليها و نعالج الاشي اول
+
+724
+00:58:09,640 --> 00:58:13,920
+بأول الدراسة اول بأول و المراجعة و انك انت تثبت
+
+725
+00:58:13,920 --> 00:58:18,040
+المعلومات هذا بفيدك اكتر و ان شاء الله يعني هيك ما
+
+726
+00:58:18,040 --> 00:58:22,420
+بيكون اشي عليكم صعب يعطيكم الف عافية و مفقين بإذن
+
+727
+00:58:22,420 --> 00:58:22,640
+الله
+
diff --git a/PL9fwy3NUQKwY9jl8xw-bWkVZkWcGGyspd/1ElgyngArXQ.srt b/PL9fwy3NUQKwY9jl8xw-bWkVZkWcGGyspd/1ElgyngArXQ.srt
new file mode 100644
index 0000000000000000000000000000000000000000..eb91e828cff2e46923aa2836f704e2508d2b2d84
--- /dev/null
+++ b/PL9fwy3NUQKwY9jl8xw-bWkVZkWcGGyspd/1ElgyngArXQ.srt
@@ -0,0 +1,3393 @@
+1
+00:00:02,190 --> 00:00:06,830
+السلام عليكم ورحمة الله وبركاته طالبات العزيزات
+
+2
+00:00:06,830 --> 00:00:10,610
+المحاضرة الماضية كانت بعنوان blood component أي
+
+3
+00:00:10,610 --> 00:00:17,250
+يعني مكونات الدم. حكينا عن مكونات الدم وأجزائه و
+
+4
+00:00:17,250 --> 00:00:21,410
+حكينا عن خلايا الدم المختلفة ما بين الـ plasma والـ
+
+5
+00:00:21,410 --> 00:00:25,650
+serum، و الـ anticoagulants مضادات التخثر اللي .. اللي
+
+6
+00:00:25,650 --> 00:00:30,010
+تم استخدامها، يعني most common في مختبرات الـ
+
+7
+00:00:30,010 --> 00:00:34,750
+hematology، وإن شاء الله اليوم محاضرتنا بعنوان
+
+8
+00:00:34,750 --> 00:00:38,650
+blood collection. كيف أنا أسحب عينة الدم أو أحصل
+
+9
+00:00:38,650 --> 00:00:43,670
+على عينة الدم من المريض؟ blood collection. المحاضرة
+
+10
+00:00:43,670 --> 00:00:48,710
+طويلة نوعاً ما، يعني أنا دي 49 slide، فلذلك ممكن أنا
+
+11
+00:00:48,710 --> 00:00:54,170
+أجزئها إلى part one و part two. طيب، طب خلينا نشوف
+
+12
+00:00:54,170 --> 00:01:00,120
+ما يتعلق بالـ blood collection. في البداية راح
+
+13
+00:01:00,120 --> 00:01:04,940
+نحكي عن contact with patient، التواصل مع المريض، the
+
+14
+00:01:04,940 --> 00:01:09,980
+patient in hospital is anxious, fearful, ill
+
+15
+00:01:09,980 --> 00:01:12,860
+health، and he is physically uncomfortable as a
+
+16
+00:01:12,860 --> 00:01:16,220
+result of his disease or injury. He is also
+
+17
+00:01:16,220 --> 00:01:19,900
+separated from his family. هلأ الـ patient عادة
+
+18
+00:01:19,900 --> 00:01:24,500
+بيكون متوتر، anxious، fearful، خايف، ill health،
+
+19
+00:01:24,500 --> 00:01:30,240
+صحته مش مساعدة، مريض، أيضاً هو غير مرتاح نتيجة مرضه
+
+20
+00:01:30,240 --> 00:01:37,100
+أو نتيجة، يعني الإصابة أو الشكوى الصحية اللي هو عم
+
+21
+00:01:37,100 --> 00:01:41,720
+بيشكى منها. هلأ طبيعي أنه يكون كمان، حتى مش بس عنده
+
+22
+00:01:41,720 --> 00:01:46,640
+تعب physical، يعني تعب واضح ظاهر على جسده، ممكن يكون
+
+23
+00:01:46,640 --> 00:01:51,200
+حتى كمان نفسيته تعبانة لإنه عنده قلق، عنده توتر بما
+
+24
+00:01:51,200 --> 00:01:57,040
+يتعلق بصحته، هل هذا العِلَّة أو المرض اللي هو مصاب به؟
+
+25
+00:01:57,040 --> 00:02:01,320
+هل هو يودي بحياته أو لا؟ He .. he is also separated
+
+26
+00:02:01,320 --> 00:02:06,700
+from his family. هو أيضاً إذا كان مريض مقيم في الـ ..
+
+27
+00:02:06,880 --> 00:02:11,300
+المستشفى، يعني موجود على أحد أسرة أقسام المستشفى،
+
+28
+00:02:11,300 --> 00:02:16,940
+فبالتالي هو بيكون بعيد عن أسرته، فهذا أيضاً بيأثر
+
+29
+00:02:16,940 --> 00:02:20,600
+على نفسيته. فبالتالي، الـ medical technologist must
+
+30
+00:02:20,600 --> 00:02:24,600
+show the patient at all times the kindness and
+
+31
+00:02:24,600 --> 00:02:28,020
+understanding that can mean some .. mean so much.
+
+32
+00:02:28,190 --> 00:02:33,190
+هلأ، يعني الـ .. الشخص، احنا كأخصائي تحاليل طبية، أنا
+
+33
+00:02:33,190 --> 00:02:39,430
+لازم أُظهر تعاطفي، وأُظهر .. أُظهر لطفي، وأيضاً تفهمي
+
+34
+00:02:39,430 --> 00:02:43,770
+لوضع المريض، اللي هذا يعني راح يكون بيعني له الكثير.
+
+35
+00:02:44,830 --> 00:02:49,430
+طيب، بالنسبة لو كان المريض هذا عبارة عن a child،
+
+36
+00:02:49,430 --> 00:02:53,750
+ممكن تكون تجربة سحب عينة من a child عبارة عن
+
+37
+00:02:53,750 --> 00:02:58,150
+terrible experience بالنسبة له، يعني تجربة سيئة،
+
+38
+00:02:58,150 --> 00:03:04,050
+أو تجربة مخيفة جداً، مريعة بالنسبة له، وممكن تكون
+
+39
+00:03:04,050 --> 00:03:06,990
+remembered and feared for that child for many
+
+40
+00:03:06,990 --> 00:03:11,230
+years. يعني أنها تظل عالقة بذاكرته وتخوفه لسنوات
+
+41
+00:03:11,230 --> 00:03:17,490
+عديدة. ليش؟ لأنه ممكن تكون هذه التجربة، تجربة سحب
+
+42
+00:03:17,490 --> 00:03:21,730
+العينة أو الحصول على العينة كانت سيئة أو مؤلمة أو
+
+43
+00:03:21,730 --> 00:03:25,190
+ما كانت بالاحتواء اللي يجب أو ينبغي أن تكون عليه.
+
+44
+00:03:25,190 --> 00:03:29,460
+Gain the child’s trust before proceeding with
+
+45
+00:03:29,460 --> 00:03:35,800
+blood collection. لازم أنه نكسب أو نكسب ثقة الطفل
+
+46
+00:03:35,800 --> 00:03:40,260
+قبل أن نبدأ بعملية سحب الدم. The child should be
+
+47
+00:03:40,260 --> 00:03:43,720
+informed of what is going to happen. If the child
+
+48
+00:03:43,720 --> 00:03:47,060
+is told that the puncture will not hurt, the child’s
+
+49
+00:03:47,060 --> 00:03:49,760
+trust will be lost because this statement is
+
+50
+00:03:49,760 --> 00:03:54,360
+generally not true. هلأ احنا طبعاً لازم نخبر
+
+51
+00:03:54,360 --> 00:04:02,340
+الطفل أنه إيش احنا راح نسوي، يعني مثلاً بالتعامل مع
+
+52
+00:04:02,340 --> 00:04:07,960
+الطفل، يعني بيكون kindly، أنا راح أدخل الإبرة وأسحب
+
+53
+00:04:07,960 --> 00:04:14,380
+منك عينة دم. طبعاً ما لازم أحكيله أنه هذا الشيء مش
+
+54
+00:04:14,380 --> 00:04:18,220
+مؤلم، لأن هو فعلياً راح يتألم، وبالتالي هيك أنا راح
+
+55
+00:04:18,220 --> 00:04:22,100
+أخسر ثقته، وراح يكون عنده terrible experience
+
+56
+00:04:22,100 --> 00:04:26,540
+يتذكرها ويخاف منها لسنوات، تمام؟ فبالتالي لازم أنا
+
+57
+00:04:26,540 --> 00:04:29,020
+مثلاً أحكيله بالتفصيل أنا إيش بدي أعمل، يعني أنا بدي
+
+58
+00:04:29,020 --> 00:04:31,620
+أسحب .. بدي .. بدي أنخزه شوية صغيرة، وبدي أسحب منك
+
+59
+00:04:31,620 --> 00:04:36,160
+عينة دم عشان نعمل لك الفحص ونتطمن عليك، بس أنت قوي،
+
+60
+00:04:36,160 --> 00:04:40,040
+مش راح تتألم كثير، هي توجع شوية صغيرة، أنخزة
+
+61
+00:04:40,040 --> 00:04:45,040
+صغيرة، وأنت، يعني كتير قوي، وأنت راح تتحمل، وأنت
+
+62
+00:04:45,040 --> 00:04:50,580
+أسد، و .. من هذا الكلام، رفعاً لمعنوياته، ونحاول أنه
+
+63
+00:04:50,580 --> 00:04:55,140
+احنا نكسب ثقته. طبعاً بالنسبة للـ phlebotomy، الـ
+
+64
+00:04:55,140 --> 00:04:58,120
+procedure، خلينا نشوف إيش معنى كلمة phlebotomy.
+
+65
+00:04:58,120 --> 00:05:03,360
+Phleb .. phleb لحالها معناها artery أو معناها
+
+66
+00:05:03,360 --> 00:05:07,680
+vein، vein، sorry، معناها vein، وريد، تمام، وto me
+
+67
+00:05:07,680 --> 00:05:13,020
+معناها، يعني to remove أو to isolate، تمام، يعني أن
+
+68
+00:05:13,020 --> 00:05:21,150
+أنا أعزل أو أستأصل أو أزيل، تمام، من الوريد، تمام، جزء
+
+69
+00:05:21,150 --> 00:05:24,790
+منه. إيش هو الجزء اللي أنا بدي أشيله من الوريد؟ الـ
+
+70
+00:05:24,790 --> 00:05:30,490
+blood، تمام. إذاً، to me، يعني عملية سحب عينة
+
+71
+00:05:30,490 --> 00:05:36,520
+دم من الوريد، هاي معناها، تمام. طيب، ونفس المعنى أيضاً،
+
+72
+00:05:36,520 --> 00:05:41,360
+vein puncture، إن أنا أسحب عينة من الوريد أيضاً،
+
+73
+00:05:41,360 --> 00:05:44,820
+معناها vein puncture. خليني أنا أشوف إيش مكتوب هنا.
+
+74
+00:05:44,820 --> 00:05:48,720
+Blood specimens، عينات الدم، are commonly obtained
+
+75
+00:05:48,720 --> 00:05:53,060
+from patients’ veins by puncture. Under some
+
+76
+00:05:53,060 --> 00:05:56,640
+circumstances، a skin puncture will be used for
+
+77
+00:05:56,640 --> 00:06:00,920
+this purpose. هلأ، أحياناً في بعض الحالات ممكن أنا
+
+78
+00:06:00,920 --> 00:06:04,600
+أعمل skin puncture لحتى أحصل على عينة الدم، يعني
+
+79
+00:06:04,600 --> 00:06:08,160
+عينة الدم غالباً commonly obtained، commonly
+
+80
+00:06:08,160 --> 00:06:13,580
+obtained، يعني غالباً تُعزل من الوريد، أو يعني يُحصل
+
+81
+00:06:13,580 --> 00:06:18,710
+عليها من الوريد، نحصل عليها من الوريد، تمام؟ ولكن في
+
+82
+00:06:18,710 --> 00:06:22,750
+بعض الحالات أنا ممكن أحصل على عينة الدم من إحداث
+
+83
+00:06:22,750 --> 00:06:27,350
+.. يعني نخز في الجلد. كلمة puncture أكيد مرت
+
+84
+00:06:27,350 --> 00:06:31,210
+معاكم، أنه مثلاً puncture عجل السيارة، puncture عجل
+
+85
+00:06:31,210 --> 00:06:35,570
+الدراجة، تمام؟ إيش يعني؟ يعني صار في ثقوب، تمام؟
+
+86
+00:06:35,570 --> 00:06:38,870
+فلما أنا أحكي vein puncture، يعني إحداث ثقوب في
+
+87
+00:06:38,870 --> 00:06:43,700
+الوريد للخروج أو للحصول على عينة الدم. إحداث ثقب في
+
+88
+00:06:43,700 --> 00:06:47,900
+الـ skin للخروج أو للحصول على عينة دم، إذاً puncture
+
+89
+00:06:47,900 --> 00:06:51,820
+يعني ثقب، تمام؟ إذاً vein puncture يعني حصول على
+
+90
+00:06:51,820 --> 00:06:57,550
+عينة دم من الوريد، تمام؟ و skin puncture حصول على
+
+91
+00:06:57,550 --> 00:07:01,610
+عينة دم من الـ skin، تمام؟ أكيد، يعني ممكن مرتوا
+
+92
+00:07:01,610 --> 00:07:06,170
+بتجربة أن تروحوا على العيادات، خصوصاً عيادات الرعاية
+
+93
+00:07:06,170 --> 00:07:10,090
+الأولية، مثل عيادات الوكالة، وأنه لما بيدوا يسحبوا
+
+94
+00:07:10,090 --> 00:07:15,910
+منكم عينة، بينخزوا الإصبع، تمام؟ ومن خلال أداة
+
+95
+00:07:15,910 --> 00:07:21,340
+شفرة خاصة للنخز، والحصول على عينة دم من خلال الـ
+
+96
+00:07:21,340 --> 00:07:25,300
+skin، من خلال، يعني أنه أنا ما .. ما .. ما استعملت
+
+97
+00:07:25,300 --> 00:07:29,940
+إبرة وما دخلت جوا الوريد، تمام؟ هاي معناها الـ skin
+
+98
+00:07:29,940 --> 00:07:35,700
+puncture. بغض النظر عن طريقة سحب العينة، irrespective
+
+99
+00:07:35,700 --> 00:07:40,420
+of the method used،
+
+100
+00:07:40,420 --> 00:07:44,820
+certain techniques are common to all phlebotomy
+
+101
+00:07:44,820 --> 00:07:48,440
+procedures. هلأ أنا لازم في عدة خطوات بدي أُحضرها،
+
+102
+00:07:48,440 --> 00:07:55,680
+بدي أُمر فيها، بدي أتأكد منها أو أسويها قبل أن أحصل على
+
+103
+00:07:55,680 --> 00:07:59,860
+عينة الدم. هذه أول شيء بدي أعمله: check the request
+
+104
+00:07:59,860 --> 00:08:04,540
+form. بدي أتأكد من الـ request form اللي جاي مع
+
+105
+00:08:04,540 --> 00:08:13,680
+العينة. هلأ في طبعاً مختبرات المستشفى بتكون مُقسّمة
+
+106
+00:08:13,680 --> 00:08:17,860
+على عدة أقسام: قسم الـ hematology، قسم الـ chemistry، الـ
+
+107
+00:08:17,860 --> 00:08:22,220
+microbiology، الـ routine، القسم الـ .. الـ serology،
+
+108
+00:08:22,220 --> 00:08:26,640
+المناعة. فبالتالي، عدة أقسام، وبيكون في عندي غرفة
+
+109
+00:08:26,640 --> 00:08:31,640
+منفصلة عنهم، اللي هي غرفة سحب العينات، بنسميها الـ
+
+110
+00:08:31,640 --> 00:08:34,340
+sample collection room، تمام؟ هي ممكن تكون أيضاً
+
+111
+00:08:34,340 --> 00:08:38,200
+كمان، يعني مش شرط تكون موجودة متصلة مع المختبر، ممكن
+
+112
+00:08:38,200 --> 00:08:41,600
+تكون، يعني المختبر يكون على الطابق الثاني، غرفة سحب
+
+113
+00:08:41,600 --> 00:08:44,940
+العينات تكون على الطابق الأول، زي مثلاً ما هو موجود
+
+114
+00:08:44,940 --> 00:08:47,200
+بمستشفى الأوروبي، تمام؟
+
+115
+00:08:49,050 --> 00:08:54,830
+فغرفة سحب العينات هذه بيصلها الـ request form. إيش
+
+116
+00:08:54,830 --> 00:08:58,450
+يعني الـ request form؟ يعني طلب سحب العينة أو طلب
+
+117
+00:08:58,450 --> 00:09:06,290
+الفحص، تمام، وبيكون طبعاً .. بيكون كتبه الطبيب في ..
+
+118
+00:09:06,290 --> 00:09:12,610
+في form خاص، نموذج خاص لعينات والفحوصات، بيشيك عليها
+
+119
+00:09:12,610 --> 00:09:16,130
+الطبيب، مثلاً أنا بدي عين .. بدي تعملي CBC أو urine
+
+120
+00:09:16,130 --> 00:09:19,710
+test أو urine analysis أو stool analysis، حسب إيش
+
+121
+00:09:19,710 --> 00:09:24,290
+نوع الفحص اللي مطلوب. فب .. هذا طلب الفحص اللي
+
+122
+00:09:24,290 --> 00:09:30,350
+بيجي، اللي بيسمى request form، بيجي مع المريض، فأنا بـ
+
+123
+00:09:30,350 --> 00:09:34,110
+.. بأطلع عليه، فبكون عارفة بناءً أنا على ما عرفتي
+
+124
+00:09:34,110 --> 00:09:38,450
+وعلمي اللي درسته، بكون عارفة إيش نوع العينة اللي
+
+125
+00:09:38,450 --> 00:09:44,670
+يجب أن نحصل عليها من المريض. فبالتالي، بصحب العينة،
+
+126
+00:09:44,670 --> 00:09:47,790
+إذا .. إذا بده عينة دم، بصحب عينة دم، إذا بده عينة
+
+127
+00:09:47,790 --> 00:09:51,510
+urine، بخليه هو يجمع عينة urine، وبعطي تعليمات حول
+
+128
+00:09:51,510 --> 00:09:54,770
+جمع عينة stool، ويجمع عينة stool، وبعطي تعليمات
+
+129
+00:09:54,770 --> 00:09:58,730
+حول الجمع. فيعني احنا ذكرنا قبل هيك أنه في عينات
+
+130
+00:09:58,730 --> 00:10:01,430
+أنا بجمعها بنفسي، في عينات أنا بعطي تعليمات للمريض
+
+131
+00:10:01,430 --> 00:10:05,810
+كيف يجمعها، وفيها تعليمات، أنا بعطي معلومات للمريض
+
+132
+00:10:05,810 --> 00:10:09,370
+أنه كيف يحضر حاله للفحص، مثلاً يصوم أو يأكل شيء معين،
+
+133
+00:10:09,370 --> 00:10:12,350
+أو يفطر على شيء معين، أو يمتنع عن أكل أشياء معينة،
+
+134
+00:10:12,350 --> 00:10:16,460
+حسب، تمام؟ فبالتالي، الـ request form، إذاً هو الطلب
+
+135
+00:10:16,460 --> 00:10:20,280
+اللي بيكتبه المريض أو بيكون هو نموذج، معادل، بس على
+
+136
+00:10:20,280 --> 00:10:24,940
+الطبيب، يعمل test، يحط صح جنب الفحص اللي هو بده إياه
+
+137
+00:10:24,940 --> 00:10:30,390
+نجريه للمريض، تمام؟ الخطوة الثانية، إذا أنا بدي أشيك
+
+138
+00:10:30,390 --> 00:10:33,630
+على هذا الـ request form، بدي أتأكد أنه معبّأ بشكل
+
+139
+00:10:33,630 --> 00:10:37,130
+صحيح أو لا من قبل الطبيب. The correct patient
+
+140
+00:10:37,130 --> 00:10:42,290
+identification. بدي أ .. أتعرف على المريض بشكل صحيح،
+
+141
+00:10:42,290 --> 00:10:45,570
+أتأكد أنه هذا هو المريض اللي أنا بدي أجمع منه
+
+142
+00:10:45,570 --> 00:10:52,950
+العينة أو لا. يعني ما أروحش أجمع عينة من مريض آخر، ويكون
+
+143
+00:10:52,950 --> 00:10:57,450
+يعني مثلاً هيك بكون أنا عاملة mix ما بين النموذج،
+
+144
+00:10:57,450 --> 00:11:00,570
+يعني يكون النموذج من مريض، والعينة من مريض آخر،
+
+145
+00:11:00,570 --> 00:11:05,010
+فبالتالي النتائج ما بتكونش معبرة، تمام؟ بيصير في خلط و
+
+146
+00:11:05,010 --> 00:11:09,590
+خربطة ما بين الـ requests forms وما بين العينات.
+
+147
+00:11:10,270 --> 00:11:13,730
+The correct specimen identification. أنا بدي أعرف
+
+148
+00:11:13,730 --> 00:11:18,550
+إيش نوع العينة اللي بدي أستخدمها، زي ما أنتو شفتوا،
+
+149
+00:11:18,550 --> 00:11:23,090
+لو مثلاً، لو بدي أسحب عينة دم، مثلاً، بدي أعرف أنا في
+
+150
+00:11:23,090 --> 00:11:27,330
+أي تيوب بدي أحطها. المحاضرة الماضية احنا تحدثنا عن
+
+151
+00:11:27,330 --> 00:11:32,020
+عدة أنواع من التيوبات، كل تيوب فيها additive، فيها
+
+152
+00:11:32,020 --> 00:11:37,340
+إضافة، فيها anticoagulant مختلف عن الأخرى، فبالتالي
+
+153
+00:11:37,340 --> 00:11:40,840
+أنا بدي أميز، فيه فحوصات بتنعمل على heparin، فيه
+
+154
+00:11:40,840 --> 00:11:44,900
+فحوصات بتنعمل على citrate، شرط لازم يكون citrate في
+
+155
+00:11:44,900 --> 00:11:48,880
+العينة، فيه فحوصات على EDTA، فحسب إيش نوع الـ
+
+156
+00:11:48,880 --> 00:11:52,400
+anticoagulant الموجود أو الـ additive الموجود فيه،
+
+157
+00:11:52,400 --> 00:11:56,300
+فحوصات لازم العينة تتجلط، وأنا أفصل serum، فلازم
+
+158
+00:11:56,300 --> 00:11:59,340
+أحطها في plain tube أو التيوبات اللي فيها clot
+
+159
+00:11:59,340 --> 00:12:03,680
+activator. فهذه كلها طبعاً أنا بدي أميزها، بدي أعرف
+
+160
+00:12:03,680 --> 00:12:08,460
+أنا إيش نوع العينة المطلوب عشان أحصل على إيش
+
+161
+00:12:08,460 --> 00:12:11,600
+correct specimen identification. أنا عرفت نوع
+
+162
+00:12:11,600 --> 00:12:18,710
+العينة الصحيح اللي أنا بدي أحصل عليه، تمام؟ فلازم
+
+163
+00:12:18,710 --> 00:12:24,150
+أيضاً أعمل إجراءات تضمن الـ safety، تضمن الأمان،
+
+164
+00:12:24,150 --> 00:12:28,710
+والسلامة، والـ infection control، والحد من انتقال
+
+165
+00:12:28,710 --> 00:12:32,350
+العدوى. لمين؟ أنا حكيت بدي أحافظ .. أحافظ على
+
+166
+00:12:32,350 --> 00:12:37,010
+سلامتي أولاً، وسلامة الـ patient، وسلامة الـ
+
+167
+00:12:37,010 --> 00:12:40,650
+colleagues، الزملاء اللي بيشتغلوا بالمختبر، تمام؟
+
+168
+00:12:40,650 --> 00:12:46,690
+وأيضاً بدي بعد هيك أبدأ أحضر مكان الـ puncture،
+
+169
+00:12:46,690 --> 00:12:50,530
+المكان اللي أنا بدي أسحب منه عينة دم، سواء كان vein،
+
+170
+00:12:50,530 --> 00:12:55,050
+أو سواء كان skin، فبدي أحضره علشان أبدأ في سحب
+
+171
+00:12:55,050 --> 00:12:59,540
+العينة، تمام؟ إذاً هذه الخطوات، بغض النظر عن عملية
+
+172
+00:12:59,540 --> 00:13:06,300
+السحب ذات نفسها، أنا لازم أقوم فيها في كل مرة بسحب
+
+173
+
+223
+00:16:57,580 --> 00:17:01,180
+requesting microbiology, cytology, fluid analysis
+
+224
+00:17:01,180 --> 00:17:05,480
+or other testing where analysis is reporting and
+
+225
+00:17:05,480 --> 00:17:11,880
+is site specific. هلأ أحيانا بيكون الطبيب معينلي ال
+
+226
+00:17:11,880 --> 00:17:15,460
+source of specimen. يعني أسحبيلي عينة من المكان
+
+227
+00:17:15,460 --> 00:17:19,080
+الفلاني. أنا بدي خصوصا إذا كان بديها لل
+
+228
+00:17:19,080 --> 00:17:22,880
+microbiology. إذا إحنا بنعمل مزرعة أو إذا كان بدنا
+
+229
+00:17:22,880 --> 00:17:27,020
+ندرس الخلايا، نشوف هل في خلايا سرطانية، يعني عفوا، أو
+
+230
+00:17:27,020 --> 00:17:35,720
+لأ، بدي أشوف فحوصات، سوائل الجسم. بده مثلا فحص من
+
+231
+00:17:35,720 --> 00:17:43,790
+جزء من الجلد ملتهب... الطبيب شاكت إنه في عدوى
+
+232
+00:17:43,790 --> 00:17:48,010
+فطرية... فطريات على الجلد، فأنا لازم من هذا المكان
+
+233
+00:17:48,010 --> 00:17:52,490
+آخد مسحة من ال... ال... فما... فما بنفع يجي...
+
+234
+00:17:52,490 --> 00:17:58,770
+يعني ما بنفع يسيب ليها يعني عائمة، مش معروفة. لازم
+
+235
+00:17:58,770 --> 00:18:03,150
+أنا يحدد لي إنه من المكان الفلاني. مثلا من الإيد
+
+236
+00:18:03,150 --> 00:18:10,650
+اليمنى فوق الرسغ، مثلا، موجود في منطقة infection.
+
+237
+00:18:10,650 --> 00:18:13,710
+منها أنا بدي سحب عينة عشان أشوف إيش نوع ال
+
+238
+00:18:13,710 --> 00:18:17,030
+infection. مثلا فطريات ولا بكتيريا أو whatever.
+
+239
+00:18:17,030 --> 00:18:21,750
+فبالتالي لما يكون المكان الـ site specific لازم أنا
+
+240
+00:18:21,750 --> 00:18:27,080
+أسحب من مكان معين. لازم هذا الطبيب يحدد لي source of
+
+241
+00:18:27,080 --> 00:18:31,200
+specimen. مصدر العينة. date and time of collection.
+
+242
+00:18:31,200 --> 00:18:38,920
+أيضا التاريخ ووقت سحب العينة لازم يكون يعني مكتوب.
+
+243
+00:18:38,920 --> 00:18:44,370
+و الـ initials of the phlebotomist. برضه كمان لازم الاسم
+
+244
+00:18:44,370 --> 00:18:48,730
+الأول من الشخص اللي سحب العينة يكون موجود أو مدكر.
+
+245
+00:18:48,730 --> 00:18:53,610
+يعني في معلومات بعديها الطبيب، يعني اسم المريض والـ
+
+246
+00:18:53,610 --> 00:18:57,390
+ID number، الـ date of birth، الـ gender، الـ
+
+247
+00:18:57,390 --> 00:19:00,450
+requisitioning physician's complete name، والـ
+
+248
+00:19:00,450 --> 00:19:02,990
+indicating test، والـ source of specimen. هدول
+
+249
+00:19:02,990 --> 00:19:07,850
+بعبيهم الطبيب. إحنا بعد ما أنا آخد الـ request من
+
+250
+00:19:07,850 --> 00:19:12,670
+المريض وأسحب له العينة، لازم أسجل الوقت والتاريخ
+
+251
+00:19:12,670 --> 00:19:17,830
+لسحب العينة، وأيضا الاسم الأول من اسمي، نور، تمام؟
+
+252
+00:19:17,830 --> 00:19:22,330
+فهكذا بكتب على الـ request form، تمام؟ إذا جزء يُعبأ
+
+253
+00:19:22,330 --> 00:19:26,510
+من خلال أو يُملى من خلال الطبيب، وجزء يُملى من خلال
+
+254
+00:19:26,510 --> 00:19:30,230
+ال... الـ phlebotomist، أو اللي هو أخصائي المختبرات
+
+255
+00:19:30,230 --> 00:19:35,610
+اللي بيجمع العينة، تمام؟ طيب، patient identification.
+
+256
+00:19:35,610 --> 00:19:40,990
+أثناء وصول الـ request، تمام؟ وأنا بدي أسحب العينة
+
+257
+00:19:40,990 --> 00:19:44,470
+من المريض، لازم أعمل حاجة اسمها patient
+
+258
+00:19:44,470 --> 00:19:49,210
+identification. أنا بدي أميز أن هو المريض اللي أنا بدي أسحب منه العينة، تمام؟ فـ is it critical for
+
+259
+00:19:49,210 --> 00:19:53,090
+a hospital patient that is accomplished by checking
+
+260
+00:19:53,090 --> 00:19:56,570
+the identification wristband? إذا الشخص مقيم على
+
+261
+00:19:56,570 --> 00:20:02,030
+... في المستشفى، أو يعني عنده بيات في المستشفى، هو
+
+262
+00:20:02,030 --> 00:20:05,790
+hospital patient، تمام؟ أو أحيانا بسميها in
+
+263
+00:20:05,790 --> 00:20:10,270
+patient. بحط كلمة in، تمام؟ بحط كلمة in هنا بيصير
+
+264
+00:20:10,270 --> 00:20:15,270
+inpatient. طبعا إذا كان كالشخص... المريض inpatient
+
+265
+00:20:15,270 --> 00:20:21,050
+يعني patient، مريض داخل المستشفى، أو hospital
+
+266
+00:20:21,050 --> 00:20:25,330
+patient. طبعا إذا كان هذا المريض في داخل المستشفى
+
+267
+00:20:25,330 --> 00:20:29,990
+على أحد الأسرة، فأنا، أو إذا كان خصوصا فاقد الوعي،
+
+268
+00:20:29,990 --> 00:20:33,810
+يعني، أو مغيب، بيكون في بإيده حاجة اسمها wristband.
+
+269
+00:20:33,810 --> 00:20:39,030
+طبعا يعني اللي على الرسغ، على الكوع، يعني لأ، مش الكوع،
+
+270
+00:20:39,030 --> 00:20:44,680
+الرسغ عند إيده هنا، بيكون في أسورة هو لابسها وفيها
+
+271
+00:20:44,680 --> 00:20:51,700
+معلوماته الخاصة، اسمه ورقمه وال... مثلا يعني أي
+
+272
+00:20:51,700 --> 00:20:58,780
+معلومات مهمة بتتعلق بالمريض،الاسم والـ hospital
+
+273
+00:20:58,780 --> 00:21:02,260
+نفس ال... الأسورة اللي بتكون في إيد المولود أول
+
+274
+00:21:02,260 --> 00:21:05,820
+ما بينولد، بن... بنكتب اسم فلان، ابنة فلانة، وال... الـ
+
+275
+00:21:05,820 --> 00:21:10,470
+number تبعه وتاريخ الولادة، ومثلا وزنه، هذه
+
+276
+00:21:10,470 --> 00:21:15,830
+معلومات بتتعلق في المريض أو بتتعلق في الـ baby اللي
+
+277
+00:21:15,830 --> 00:21:18,690
+انولد، تمام؟ فأيضا نفس الشيء، المريض اللي بيكون على
+
+278
+00:21:18,690 --> 00:21:22,510
+أسرة المستشفى بيكون في عنده... المفروض يكون في
+
+279
+00:21:22,510 --> 00:21:25,170
+عنده wristband، يعني أسورة في إيده، ومكتوب فيها على
+
+280
+00:21:25,170 --> 00:21:30,200
+الأقل الاسم والـ hospital وبعض المعلومات المتعلقة
+
+281
+00:21:30,200 --> 00:21:33,480
+فيه. فأنا لما آجي بدي أسحب عينة من مريض، خصوصا
+
+282
+00:21:33,480 --> 00:21:37,400
+لو كان فاقد للوعي أو مغيب، بدي أcheck عليها
+
+283
+00:21:37,400 --> 00:21:41,040
+الأسورة وأشوف المعلومات اللي عليها مطابقة
+
+284
+00:21:41,040 --> 00:21:44,800
+لـ request عشان أعرف إن هو هذا المريض اللي بدي
+
+285
+00:21:44,800 --> 00:21:46,920
+أسحب منه. أو طبعا إذا كان in patient صاحي، بقوله
+
+286
+00:21:46,920 --> 00:21:51,720
+بدي أتكلم معه، بدي أحكيله أنت فلان، إيش اسمك؟ طب إيش
+
+287
+00:21:51,720 --> 00:21:55,520
+رقمك؟ طب إيش اسمك بالكامل؟ قد إيش عمرك؟ عم بدي أسأله
+
+288
+00:21:55,520 --> 00:22:01,980
+بعض الأسئلة، أكرر بعض الأسئلة اللي موجودة في الـ
+
+289
+00:22:01,980 --> 00:22:05,280
+request عشان أتأكد إنه هو هذا الشخص اللي موجود...
+
+290
+00:22:05,280 --> 00:22:09,060
+اللي مذكور له أو اللي مشار له في الـ request. بدي
+
+291
+00:22:09,060 --> 00:22:12,120
+أسحب منه عينة أو لأ، طبعا؟ طيب، إذا كان المريض عبارة
+
+292
+00:22:12,120 --> 00:22:17,930
+عن outpatient، إيش يعني outpatient؟ يعني هو مريض
+
+293
+00:22:17,930 --> 00:22:21,650
+خارج المستشفى، إذا إجا... إجا لحتى يزور المستشفى،
+
+294
+00:22:21,650 --> 00:22:27,810
+يعني إجا على العيادات الخارجية للمستشفى، تمام؟ وبده
+
+295
+00:22:27,810 --> 00:22:32,560
+يتعالج ويطلب له فحص مثلا معين، فساعتها أنا بدي
+
+296
+00:22:32,560 --> 00:22:38,040
+أتعامل مع المريض هذا، بدي أعمل... بدي أسأله، ask the
+
+297
+00:22:38,040 --> 00:22:42,880
+patient for his or her full name. بدي أسأله عن اسمه
+
+298
+00:22:42,880 --> 00:22:47,260
+بالكامل، عن بعض المعلومات المتعلقة، to be verified،
+
+299
+00:22:47,260 --> 00:22:51,940
+عشان أتأكد إنه هذا هو الشخص المعني بالـ request form،
+
+300
+00:22:51,940 --> 00:22:59,590
+أو هو الشخص اللي... اللي لازم أنا أسحب منه عينة، وهذا الـ
+
+301
+00:22:59,590 --> 00:23:04,070
+request له أو لأ، تمام؟ إذا نوعي المرضى،
+
+302
+00:23:04,070 --> 00:23:08,630
+نعين، إما بيكون inpatient أو outpatient، تمام؟ الـ
+
+303
+00:23:08,630 --> 00:23:12,470
+inpatient ممكن يكون فاقد للوعي، نايم على الأسرة،
+
+304
+00:23:12,470 --> 00:23:17,630
+لا يمكن التواصل معاه، verbal communication، مش
+
+305
+00:23:17,630 --> 00:23:21,370
+عارفة أتكلم معاه، فبدي أشيك على wristband، إذا كان هو
+
+306
+00:23:21,370 --> 00:23:25,830
+صاحي، فبسأله. أما الـ outpatient، طالما هو خارج
+
+307
+00:23:25,830 --> 00:23:30,110
+المستشفى وإيجي على العيادات الخارجية، إذا إن هو
+
+308
+00:23:30,110 --> 00:23:32,410
+إيجي واعي، معناها بدي أسأله اسمه بالكامل، بعض
+
+309
+00:23:32,410 --> 00:23:39,150
+المعلومات اللي موجودة في الـ request، وعشان يأكد لي
+
+310
+00:23:39,150 --> 00:23:42,870
+إن هذا الـ request له، زي تاريخ ميلاده، مكان سكنه، أي
+
+311
+00:23:42,870 --> 00:23:47,570
+شيء بيتعلق فيه داخل الـ request، بسأله عنه عشان أشوف
+
+312
+00:23:47,570 --> 00:23:51,250
+إجابته صحيحة أو لأ، هو هذا الشخص اللي... اللي طلب
+
+313
+00:23:51,250 --> 00:23:54,330
+له أو لأ، تمام؟ طيب، هذا... إذن هذا بالنسبة للـ
+
+314
+00:23:54,330 --> 00:23:59,290
+patient identification. بالنسبة للـ specimen
+
+315
+00:23:59,290 --> 00:24:02,150
+identification، لو العينة أجتني من غرفة سحب، على
+
+316
+00:24:02,150 --> 00:24:06,390
+المختبرات، تمام؟ فمعناها العينة لازم تكون إشمالها...
+
+317
+00:24:06,390 --> 00:24:11,310
+... سيريلها labelled، معلمة، أو صار... عملنا لها
+
+318
+00:24:11,310 --> 00:24:16,370
+label، كتبنا عليها معلومات مهمة لحتى ما يتم فقدانها
+
+319
+00:24:16,370 --> 00:24:21,510
+أو ضياعها أو خربطتها أو يعني أنها يتم استبدالها
+
+320
+00:24:21,510 --> 00:24:26,710
+بعينات أخرى. عملية الـ labeling مهمة جدا يا بنات،
+
+321
+00:24:26,710 --> 00:24:32,040
+فلازم أنا أعمل labeling. بِشو؟ بالـ patients' surname،
+
+322
+00:24:32,040 --> 00:24:36,240
+first and middle name. يعني نفس الاسم بنفس الطريقة
+
+323
+00:24:36,240 --> 00:24:40,120
+الكتابة اللي موجودة في الـ request، بكتبها على
+
+324
+00:24:40,120 --> 00:24:43,140
+العينة. أيضا الـ ID number، الرقم المعرف للمريض، سواق
+
+325
+00:24:43,140 --> 00:24:50,880
+كان رقم هويته أو رقم الطبيب أو رقم ملفه، حسب يعني
+
+326
+00:24:50,880 --> 00:24:56,390
+المستشفى، إيش نظامها في تعريف المرضى. في ناس في
+
+327
+00:24:56,390 --> 00:25:00,170
+مستشفيات بتعرف المرضى من خلال رقم هويتهم، في لأ
+
+328
+00:25:00,170 --> 00:25:03,350
+بتعطيهم رقم، هذا رقم الطبيب أو رقم ملفه، تمام؟
+
+329
+00:25:03,350 --> 00:25:08,810
+date, time and initials of the phlebotomist. لازم
+
+330
+00:25:08,810 --> 00:25:11,970
+أيضا الجزئية اللي بدي أعبيها أنا على الـ request اللي
+
+331
+00:25:11,970 --> 00:25:15,050
+هي تاريخ ووقت سحب العينة والـ initials تبعي، اسم
+
+332
+00:25:15,050 --> 00:25:19,250
+الأول، لازم أيضا أكتبها على العينة، عشان لو في أي
+
+333
+00:25:19,250 --> 00:25:23,520
+مشاكل يرجعوا لي، تمام؟ أي خربطة أو أي مشاكل
+
+334
+00:25:23,520 --> 00:25:27,920
+يرجعوا لي، مثلا ب... بتعلق بسحب العينة، عشان أنا
+
+335
+00:25:27,920 --> 00:25:31,840
+أفيدهم. طيب،
+
+337
+00:25:37,770 --> 00:25:42,370
+Safety and infection control. أيضا لازم... لازم
+
+338
+00:25:42,370 --> 00:25:47,910
+أراعي ال... ال... الأمان والسلامة، وأيضا أحسب
+
+339
+00:25:47,910 --> 00:25:52,490
+انتقال العدوى في... أثناء سحب العينة، تمام؟ أثناء
+
+340
+00:25:52,490 --> 00:25:57,610
+الحصول على العينة. فبالتالي لازم، طبعا أنا لما أبدأ
+
+341
+00:25:57,610 --> 00:26:01,450
+أتواصل مع ال... ال... الـ sick patient وعيناتهم.
+
+342
+00:26:01,450 --> 00:26:05,450
+طبعا أنا بسحب عينة دم، يا بنات، عينة دم، يعني أنا
+
+343
+00:26:05,450 --> 00:26:09,010
+معرضة لمثلا إنه لا سمح الله، لا سمح الله، إذا
+
+344
+00:26:09,010 --> 00:26:14,550
+المريض مثلا مصاب بـ hepatitis، بـ virus، الـ AIDS، بأي
+
+345
+00:26:14,550 --> 00:26:18,990
+فيروس خطير ممكن ينتقل لي عبر الدم، تمام؟ إذا لو
+
+346
+00:26:18,990 --> 00:26:23,150
+مثلا أنا ما اتبعت قواعد السلامة والأمان، إذا
+
+347
+00:26:23,150 --> 00:26:26,550
+جرح صغير موجود في إيدي أثناء عملية سحب الدم،
+
+348
+00:26:26,550 --> 00:26:31,350
+معناها أنا هيك أذيت نفسي، تمام؟ فبالتالي عشان هيك
+
+349
+00:26:31,350 --> 00:26:35,210
+أنا بأراعي، بُعامل مع إنه أنا بصحى بعينة الدم إن
+
+350
+00:26:35,210 --> 00:26:39,880
+إنها هي عينة معدية، وبكون لابسة الـ gloves، ال... الـ
+
+351
+00:26:39,880 --> 00:26:45,440
+gauntlets، القفازات، وبستخدم الكحول للتعقيم وبحافظ على
+
+352
+00:26:45,440 --> 00:26:50,240
+نظافة المكان لحتى ما أنقل لنفسي العدوى، تمام؟
+
+353
+00:26:50,240 --> 00:26:55,060
+الشيء مش مخيف وما أن... يعني أنا مش بصدد أن
+
+354
+00:26:55,060 --> 00:27:00,050
+أعمل لكم تهويل. هلأ الحمد لله، قلنا سنوات في
+
+355
+00:27:00,050 --> 00:27:04,050
+المجال، والحمد لله يعني محافظين على أنفسنا، وسهل
+
+356
+00:27:04,050 --> 00:27:07,370
+جدا إنّكم تتبعوا الخطوات للسلامة والأمان و
+
+357
+00:27:07,370 --> 00:27:11,430
+تتحافظوا على أنفسكم، ولكن يعني زي ما إحنا ما بدنا
+
+358
+00:27:11,430 --> 00:27:16,450
+نهول الأمر، برضه كمان ما بدنا نستصغر الأمور ونستهين
+
+359
+00:27:16,450 --> 00:27:20,610
+فيها. فبالتالي نقدر نحمي أنفسنا. يعني فقط عليكم اتباع
+
+360
+00:27:20,610 --> 00:27:25,190
+الخطوات المهمة واللي بتتعلق بالسلامة والأمان و
+
+361
+00:27:25,190 --> 00:27:28,330
+الحد من انتشار العدوى، وهيك أنتم بتكونوا في ال...
+
+362
+00:27:28,330 --> 00:27:31,650
+الدائرة الخضراء أو في ال... في الـ safe line، إن شاء
+
+363
+00:27:31,650 --> 00:27:38,800
+الله. طبعا أنا إيش بيلزمني؟ بيلزمني إنه to protect
+
+364
+00:27:38,800 --> 00:27:42,620
+myself، to protect the other colleagues، والـ
+
+365
+00:27:42,620 --> 00:27:50,460
+visitors. يعني بدي أحمي زملائي، زوّار المختبر، العمال،
+
+366
+00:27:50,460 --> 00:27:55,520
+النظافة، المراسلين، كل حد بيورد على المختبر، أيضا بدي
+
+367
+00:27:55,520 --> 00:27:59,320
+أحميه، تمام؟ يعني بدي أحمي البيئة المحيطة فيا، و
+
+368
+00:27:59,320 --> 00:28:03,580
+أنظفها وأحاول إن أنا يكون بيئتي... فنضيفة، سليمة،
+
+369
+00:28:03,580 --> 00:28:07,740
+آمنة، لحتى إنه ما أؤذي غيري، وأيضا to protect the
+
+370
+00:28:07,740 --> 00:28:10,320
+patient، بدي أحمي patient نفسه، يعني ما أكون
+
+371
+00:28:10,320 --> 00:28:13,500
+أنا عم بنقل عدوى من مريض إلى آخر ومن آخر إلى آخر
+
+372
+00:28:13,500 --> 00:28:18,920
+وهكذا. how to protect yourself؟ كيف بدك تحمي نفسك؟
+
+373
+00:28:18,920 --> 00:28:22,800
+حكينا الشيء إحنا مش بصدد التهويل، لكن أيضا ما بدنا
+
+374
+00:28:22,800 --> 00:28:26,760
+نستهين في الأمور، فقط علينا اتباع الخطوات المهمة و
+
+375
+00:28:26,760 --> 00:28:32,040
+التعليمات المهمة لحتى نحمي أنفسنا. wear gloves and
+
+376
+00:28:32,040 --> 00:28:35,460
+lab coat or change gloves after each patient or
+
+377
+00:28:35,460 --> 00:28:39,710
+when contaminated. أول حاجة بدي أرتدي الـ gloves اللي
+
+378
+00:28:39,710 --> 00:28:44,330
+هي القفازات الطبية والـ lab coat تبعي، ويفضل إن أنا
+
+379
+00:28:44,330 --> 00:28:47,930
+أغير الـ gloves بين كل مريض والآخر، يعني كل ما أقع
+
+380
+00:28:47,930 --> 00:28:50,570
+بالمريض، يعني أنا سحبت عينة من مريض، آجي المريض اللي
+
+381
+00:28:50,570 --> 00:28:54,830
+بعده، يفضل إن أنا أشيل الـ gloves، أتخلص منها، وألبس
+
+382
+00:28:54,830 --> 00:28:59,550
+gloves جديدة. طبعا إذا تلوثت أيضا لازم أنا أتخلص
+
+383
+00:28:59,550 --> 00:29:04,710
+منها. wash hands frequently. لازم أنا أغسل إيدي ب...
+
+384
+00:29:04,710 --> 00:29:08,990
+ب... بكثرة. يعني أنا مثلا بين مريض وآخر أو بين...
+
+385
+00:29:08,990 --> 00:29:13,330
+بين... بين فت... من فترة إلى آخر، يفضل إن أنا أغسل
+
+386
+00:29:13,330 --> 00:29:16,250
+إيدي. أغسل إيدي أول ما أفوت المختبر وقبل ما أخرج
+
+387
+00:29:16,250 --> 00:29:20,430
+من المختبر أو أغادر المختبر، بين كل فترة وتانية.
+
+388
+00:29:20,430 --> 00:29:23,350
+مثلا بين كل مريضين، ثلاثة، أربعة، أنا ب... بفضل إن
+
+389
+00:29:23,350 --> 00:29:27,520
+أنا أروح أغسل إيدي وأرجع، تمام؟ على أساس إنه هيك
+
+390
+00:29:27,520 --> 00:29:31,980
+أنا بكون عم بحمي حالي، تمام؟ عم بحمي حالي. dispose
+
+391
+00:29:31,980 --> 00:29:35,780
+of items in appropriate containers. يعني إنه أنا لما
+
+392
+00:29:35,780 --> 00:29:40,960
+بدي أتخلص من أدوات السحب، تمام؟ dispose items، يعني
+
+393
+
+445
+00:33:37,670 --> 00:33:42,710
+فهي يعني وأحيانًا بنستخدم.. وبنستخدم الكحول و
+
+446
+00:33:42,710 --> 00:33:45,450
+بنستخدم الـ ditol أيضًا في عملية التعقيم
+
+447
+00:33:45,450 --> 00:33:50,930
+اللي هي التعقيم. طيب if you stick yourself with
+
+448
+00:33:50,930 --> 00:33:54,750
+a contaminated needle إذا إن صار لسمح الله ونخست
+
+449
+00:33:54,750 --> 00:33:58,910
+نفسك بإبرة ملوثة contaminated needle إيش بدنا
+
+450
+00:33:58,910 --> 00:34:04,570
+نعمل؟ أول حاجة remove your gloves تمام؟ لازم أنا
+
+451
+00:34:04,570 --> 00:34:09,210
+أرفع الـ gloves يعني أشيل الـ gloves وأتخلص منهم
+
+452
+00:34:09,210 --> 00:34:15,880
+بالسلة تمام؟ بعد هيك بدي أعمل sequencing للـ
+
+453
+00:34:15,880 --> 00:34:19,840
+puncture site to promote bleeding، بدي أعصر منطقة الـ
+
+454
+00:34:19,840 --> 00:34:24,860
+puncture، يعني بدي أضغط عليها لحتى أحفظ أنه
+
+455
+00:34:24,860 --> 00:34:28,300
+يصير في عندي bleeding، بعد هيك بدي.. يعني هيك
+
+456
+00:34:28,300 --> 00:34:33,150
+بيكون الدم هو عم بيطلع تمام وعم بيسير له bleeding و
+
+457
+00:34:33,150 --> 00:34:38,110
+بيطلع من الجرح اللي أنا نخست نفسي فيه، هيك بيكون عم
+
+458
+00:34:38,110 --> 00:34:41,990
+بيعمل wash لمكان الجرح، يعني في احتمال كبير إنه الـ
+
+459
+00:34:41,990 --> 00:34:47,670
+microbe اللي فيه تطلع، wash the area well with
+
+460
+00:34:47,670 --> 00:34:53,500
+soap and water بدي أغسل المكان بالميّة والصابون و
+
+461
+00:34:53,500 --> 00:35:00,420
+بدي أسجل اسم المريض والـ ID number عشان أتبع معاه
+
+462
+00:35:00,420 --> 00:35:05,960
+أشوف هذا المريض إيش عنده وإيش راح يصير معايا في
+
+463
+00:35:05,960 --> 00:35:10,720
+المستقبل، من ماذا يحمل لي المستقبل من مفاجآت متعلقة
+
+464
+00:35:10,720 --> 00:35:14,780
+بهذا المريض والـ..الـ..النخزة اللي أنا نخزت
+
+465
+00:35:14,780 --> 00:35:22,120
+فيها نفسي من الـ needle الملوثة، بدعمهم protect the
+
+466
+00:35:22,120 --> 00:35:27,840
+patient كيف أنا بدي أحمي المريض تمام، place blood
+
+467
+00:35:27,840 --> 00:35:35,400
+collection equipment away from patient، sorry، place
+
+468
+00:35:35,400 --> 00:35:39,280
+blood collection equipment away from patients
+
+469
+00:35:39,280 --> 00:35:43,300
+especially children and psychiatric patients، لازم
+
+470
+00:35:43,300 --> 00:35:48,160
+أنا أبعد أدوات سحب الدم عن المريض، خصوصًا لو كان هذا
+
+471
+00:35:48,160 --> 00:35:52,120
+المريض طفل أو مريض نفسي، يعني أدوات حادة أنا بستعمل
+
+472
+00:35:52,120 --> 00:35:57,500
+chauffeur، بستعمل needles، إبر، ممكن يكون هذا المريض
+
+473
+00:35:57,500 --> 00:36:00,840
+يعني إذا كان مثلًا مريض نفسي psychiatric، فبالتالي
+
+474
+00:36:00,840 --> 00:36:04,320
+ممكن يأذي نفسه، إذا كان طفل ممكن يلعب فيها من باب
+
+475
+00:36:04,320 --> 00:36:08,260
+الفضول، وأيضًا يأذي نفسه، فبالتالي أنا بدي أبعد هذه
+
+476
+00:36:08,260 --> 00:36:12,620
+الأدوات عن المريض، practice hygiene for the patient
+
+477
+00:36:12,620 --> 00:36:18,380
+protection، بدي أعقم المكان، السطح، أي مكان بيلامس
+
+478
+00:36:18,380 --> 00:36:23,320
+المريض أو بيحط عليه إيدي، لازم أنا أكون معقمة، بدي
+
+479
+00:36:23,320 --> 00:36:27,380
+ألبس gloves وأبدلهم مثل ما حكينا بين كل مريض و
+
+480
+00:36:27,380 --> 00:36:31,180
+الآخر، change them between each patient and wash
+
+481
+00:36:31,180 --> 00:36:36,300
+your hands frequently وأغسل إيديا باستمرار من
+
+482
+00:36:36,300 --> 00:36:39,460
+فترة لأخرى، علشان أيضًا كمان أحمي نفسي وأحمي
+
+483
+00:36:39,460 --> 00:36:43,420
+المريض، always wear a clean lab coat or gown، يعني
+
+484
+00:36:43,420 --> 00:36:48,460
+أنا بدي ألبس على طول الـ lab coat، طبيعي يكون نظيف
+
+485
+00:36:48,460 --> 00:36:54,380
+ما يكون ملوث، وبعد هيك ببدأ بعملية preparation of the
+
+486
+00:36:54,380 --> 00:36:59,360
+puncture site كيف أنا أسحب أو أحضر مكان السحب
+
+487
+00:36:59,360 --> 00:37:05,300
+سواء كان vein puncture أو skin puncture، بدي أعقم
+
+488
+00:37:05,300 --> 00:37:11,920
+المكان باستخدام 70% isopropanol، طبعًا 70%
+
+489
+00:37:11,920 --> 00:37:16,860
+isopropanol يا بنات، أو تمام، مش شرط يعني 70%
+
+490
+00:37:16,860 --> 00:37:23,920
+isopropanol، ممكن يكون 70% isopropanol or ethanol،
+
+491
+00:37:23,920 --> 00:37:29,000
+طبعًا؟ يعني 70% isopropanol أو 70% ethanol، نفس
+
+492
+00:37:29,000 --> 00:37:31,800
+الشيء، طبعًا؟ نفس الشيء
+
+493
+00:37:34,230 --> 00:37:41,910
+تمام، إذا أنا بعقم المكان بأمسحه بـ 70% ethanol و
+
+494
+00:37:41,910 --> 00:37:48,510
+طبعًا بعد ما أنا أعقم، لازم المنطقة اللي عقمتها
+
+495
+00:37:48,510 --> 00:37:53,030
+أتركها جافّة، يعني أترك مجال للـ.. للكحول يتبخر أو
+
+496
+00:37:53,030 --> 00:37:59,010
+أني أمسح.. أرجع أمسح المنطقة بـ sterile gauze، يعني
+
+497
+00:37:59,010 --> 00:38:05,220
+بشاش أو بقطن معقم، أجفف لحتى أنشف المكان، أجففه
+
+498
+00:38:05,220 --> 00:38:13,720
+عشان يكون هيك صار نظيف معقم، وبالتالي جاهز لسحب
+
+499
+00:38:13,720 --> 00:38:19,620
+العينة، بمجرّد يا بنات إنه أنا أكون عقمت المكان، سواء
+
+500
+00:38:19,620 --> 00:38:26,760
+كان منطقة الوريد أو الـ..الـ skin، الإصبع، يعني
+
+501
+00:38:26,760 --> 00:38:29,800
+سواء من الوريد، يعني من منطقة الـ..الـ.. اللي هي
+
+502
+00:38:29,800 --> 00:38:34,840
+عندي المفصل، الكوع، أو من منطقة الـ..الـ.. الأصابع
+
+503
+00:38:34,840 --> 00:38:40,200
+فبمجرد ما أعقمها، ممنوع ألمسها مرة ثانية بإيدي
+
+504
+00:38:40,200 --> 00:38:45,060
+لأنه هيك أنا بكون عم بـ ألوثها مرة ثانية، إحنا في
+
+505
+00:38:45,060 --> 00:38:49,420
+ميكروبات بتكون موجودة على جسمنا، وهاي الميكروبات
+
+506
+00:38:49,420 --> 00:38:53,490
+ممكن تكون.. ما تكونش ممرضة على الـ skin تبعنا، طبعًا،
+
+507
+00:38:53,490 --> 00:38:57,250
+لكن إذا فاتت للدم، إذا أنا لوثت فيها منطقة السحب
+
+508
+00:38:57,250 --> 00:39:00,790
+الدم وفاتت للدم، ممكن تصير ممرضة في داخل الدم،
+
+509
+00:39:00,790 --> 00:39:04,730
+طبعًا، فبالتالي لازم.. عشان هيك أنا عم بعقم منطقة
+
+510
+00:39:04,730 --> 00:39:06,550
+الـ skin قبل السحب
+
+511
+00:39:12,960 --> 00:39:18,080
+طيب نيجي لـ الـ..الـ micro sample technique، micro
+
+512
+00:39:18,080 --> 00:39:21,400
+sample يا بنات، يعني إنه أنا بدي أحصل على عينة
+
+513
+00:39:21,400 --> 00:39:27,260
+صغيرة الحجم، يعني أنا بدي أسحب عينة من الـ skin، طبعًا
+
+514
+00:39:27,260 --> 00:39:31,340
+is..refers to blood collection by skin puncture
+
+515
+00:39:31,340 --> 00:39:36,240
+يعني أنا بدي أسحب عينة الدم من خلال الـ skin puncture
+
+516
+00:39:36,240 --> 00:39:40,720
+طبعًا من خلال أني أحصل.. أعمل نخزة في الـ skin، في
+
+517
+00:39:40,720 --> 00:39:43,960
+الإصبع يعني مثلًا، أو في كعب الرجل عند المريض، عند
+
+518
+00:39:43,960 --> 00:39:47,400
+الأطفال، حسب وين المكان المهم في الـ skin، مش في
+
+519
+00:39:47,400 --> 00:39:52,140
+منطقة وريد، and is frequently used in the following
+
+520
+00:39:52,140 --> 00:39:56,800
+types of patients، طبعًا يا بنات، هذه العينة وهذا..هذا
+
+521
+00:39:56,800 --> 00:40:01,800
+موضوع سؤال امتحان، يعني أنا امتى أو من مين أو إيش
+
+522
+00:40:01,800 --> 00:40:06,700
+مين هم المرضى اللي أنا بضطر أسحب منهم micro sample
+
+523
+00:40:06,700 --> 00:40:10,820
+technique أو بضطر أسحب منهم عينة من خلال skin
+
+524
+00:40:10,820 --> 00:40:14,440
+puncture، يعني ما بقدر أسحب لهم vein puncture، بسحب لهم
+
+525
+00:40:14,440 --> 00:40:18,740
+skin puncture، أول شيء الأطفال، الـ infant، حديثي
+
+526
+00:40:18,740 --> 00:40:24,120
+الولادة، أقل من 6 شهور، أطفال يعني مولودين، مولود أقل
+
+527
+00:40:24,120 --> 00:40:28,180
+من 6 شهور في العمر، طبعًا ما عندهمش large blood
+
+528
+00:40:28,180 --> 00:40:33,180
+supply، ما عندهمش كمية دم كبيرة في جسمهم، لأن حجمهم
+
+529
+00:40:33,180 --> 00:40:38,940
+صغير، إحنا حكينا حجم الدم يعني بيتراوح حسب الكتلة
+
+530
+00:40:38,940 --> 00:40:42,580
+تبع الجسم، طب إذا هو طفل صغير، معناها حجم الدم عنده
+
+531
+00:40:42,580 --> 00:40:50,040
+أيضًا صغير، تمام؟ والـ.. بالتالي بيكون خطر عليه إن
+
+532
+00:40:50,040 --> 00:40:54,660
+أنا أسحب عينة من الوريد، تمام؟ فبضطر أنا أسحب له من
+
+533
+00:40:54,660 --> 00:40:58,260
+الـ skin، هذا الطفل إما بنخزه من كعب رجله أو من
+
+534
+00:40:58,260 --> 00:41:03,150
+الإصبع، تمام؟ in younger children، في الأطفال الأكبر
+
+535
+00:41:03,150 --> 00:41:09,530
+شوي بالعمر، ولكن الفحص المطلوب لهم محتاج small
+
+536
+00:41:09,530 --> 00:41:14,170
+amount of blood، يعني هم صح أطفال أكبر وعندهم
+
+537
+00:41:14,170 --> 00:41:19,230
+blood supply أكبر، ولكن أنا محتاجة أصلاً للفحص small
+
+538
+00:41:19,230 --> 00:41:23,410
+amount of blood، تمام، طالما أنا محتاجة small amount
+
+539
+00:41:23,410 --> 00:41:27,730
+of blood، فالـ skin puncture هي الخيار تبعي، بعمل
+
+540
+00:41:27,730 --> 00:41:32,650
+أسحب لهم من خلال الـ skin puncture، أو when adult has
+
+541
+00:41:32,650 --> 00:41:37,770
+poor vein، لما يكون شخص adult،  ده راجل كبير يعني عنده
+
+542
+00:41:37,770 --> 00:41:43,310
+أوردة وعنده كمية دم كافية، ولكن الـ..الوريد مش
+
+543
+00:41:43,310 --> 00:41:47,310
+أنا..مش عم..مش عم أستطيع أو مش عم بقدر إنه أنا
+
+544
+00:41:47,310 --> 00:41:53,500
+أسحب له عينة من الوريد، تمام؟ إيه؟ ليش؟ مثلًا ممكن
+
+545
+00:41:53,500 --> 00:41:57,660
+يكون منطقة الوريد صار لها burned، يعني انحرقت، عنده
+
+546
+00:41:57,660 --> 00:42:02,120
+حروق يعني في جسمه كلها أو في مغطية حجم كبير من
+
+547
+00:42:02,120 --> 00:42:06,720
+جسمه، فبالتالي ده يعني صعب أصل للوريد، فبالتالي صعب
+
+548
+00:42:06,720 --> 00:42:11,600
+أني أنا أسحب له عينة من الوريد، الناس اللي بتاخد
+
+549
+00:42:11,600 --> 00:42:17,240
+chemotherapy، لمرضى الـ cancer اللي بيتعالجوا بالـ
+
+550
+00:42:17,240 --> 00:42:24,270
+chemotherapy، بالكيماوي، فبالتالي هدول بيصير عندهم
+
+551
+00:42:24,270 --> 00:42:27,770
+ضعف في الأوردة، الأوردة تبعتهم بتصير رفيعة ودقيقة
+
+552
+00:42:27,770 --> 00:42:33,390
+جدًا، فبالتالي صعب إني يعني أ..أ.. أحصل عليها
+
+553
+00:42:33,390 --> 00:42:38,250
+أو أدور عليها، وصعب إني أفوت فيها إبرة، فبالتالي
+
+554
+00:42:38,250 --> 00:42:41,770
+بأخذ منهم من الـ skin puncture كمان
+
+555
+00:42:51,200 --> 00:42:55,440
+تمام؟ إيش الحاجات اللي..اللي بتلزمني أو الأدوات
+
+556
+00:42:55,440 --> 00:42:58,260
+اللي بتلزمني علشان أنا أسحب عينة من الـ skin
+
+557
+00:42:58,260 --> 00:43:02,420
+puncture؟ أول حاجة بتلزمني يعني زي الـ reagents والـ
+
+558
+00:43:02,420 --> 00:43:05,920
+equipment، أول شيء بتلزمني 70% حكينا
+
+559
+00:43:05,920 --> 00:43:09,980
+isopropyl alcohol، يعني isopropanol أو 70%
+
+560
+00:43:09,980 --> 00:43:15,250
+مية ethanol، the same، تمام؟ بتلزمني sterile gauze
+
+561
+00:43:15,250 --> 00:43:21,470
+باد، يعني قطن أو شاش معقم، وحتى أحطه على مكان
+
+562
+00:43:21,470 --> 00:43:27,270
+السحب العيني، يعني بعد ما أخلص سحب وأزيل الـ needle
+
+563
+00:43:27,270 --> 00:43:35,880
+بدي أحطه مكانها، بتلزمني أيضًا مثلًا الـ..يعني
+
+564
+00:43:35,880 --> 00:43:39,980
+plasters عشان أحطها للمريض أيضًا على مكان السحب
+
+565
+00:43:39,980 --> 00:43:44,340
+gloves، وهذه الـ gloves أنا في..عندي تلات أنواع من
+
+566
+00:43:44,340 --> 00:43:48,360
+الـ gloves، إما مصنوعة من الـ latex وهي الأكثر شيوعًا
+
+567
+00:43:48,360 --> 00:43:52,540
+أو مصنوعة من الـ nitrile أو الـ vinyl، راح نحكي عنهم
+
+568
+00:43:52,540 --> 00:43:56,160
+في الـ slide الجاي، ونشوف إيش الفرق ما بينهم، تمام؟
+
+569
+00:43:57,210 --> 00:44:00,910
+warm to protect the patient and phlebotomist، طبعًا
+
+570
+00:44:00,910 --> 00:44:04,970
+الـ gloves بتحمي المريض وبتحمي الـ..الـ..الـ
+
+571
+00:44:04,970 --> 00:44:09,070
+أخصائي المختبرات، sterile disposable blood lancet
+
+572
+00:44:09,070 --> 00:44:14,070
+طبعًا أنا بتلزمني الـ..في الـ..سحب الـ skin
+
+573
+00:44:14,070 --> 00:44:16,330
+puncture أو في سحب عينة من الـ skin puncture
+
+574
+00:44:16,330 --> 00:44:20,780
+بتلزمني الـ..الـ lancet طبعًا، إيش هي الـ lancet اللي
+
+575
+00:44:20,780 --> 00:44:26,160
+هي؟ شفرة مصممة، يعني مدببة الطرف، مصممة لإحداث ثقوب أو
+
+576
+00:44:26,160 --> 00:44:32,680
+نخز في الـ skin لحتى نحصل على عينة دم، طبعًا latex اي
+
+577
+00:44:32,680 --> 00:44:36,040
+gloves، إيش الـ latex gloves؟ طبعًا مصنوعة من الـ
+
+578
+00:44:36,040 --> 00:44:40,260
+rubber، طبعًا وبتستخدم إما في المجال الطبي أو
+
+579
+00:44:40,260 --> 00:44:43,900
+المجال الصناعي، ولكن عندي مشكلة إنها ممكن تسبب
+
+580
+00:44:43,900 --> 00:44:47,840
+حساسية، طبعًا وهي الأكثر شيوعًا، طبعًا أكثر استخدامًا
+
+581
+00:44:48,450 --> 00:44:51,910
+اللي هي الـ gloves اللي لونها أبيض، تمام؟ الـ
+
+582
+00:44:51,910 --> 00:44:55,910
+nitrile gloves، طبعًا ما فيش فيها latex، يعني ما فيش
+
+583
+00:44:55,910 --> 00:44:58,930
+فيها powder، الـ latex يعني فيها powder يا بنات،
+
+584
+00:44:58,930 --> 00:45:02,510
+تمام؟ مصنوعة من المطاط وفيها powder من الداخل، الـ
+
+585
+00:45:02,510 --> 00:45:05,630
+..طبعًا الـ..الـ nitrile gloves ما فيش فيها powder،
+
+586
+00:45:05,630 --> 00:45:10,300
+تمام؟ مصنوعة من الـ..الـ..الـ synthetic rubber، يعني
+
+587
+00:45:10,300 --> 00:45:13,660
+الـ..المطاط الصناعي، مش المطاط الطبيعي، هذه مطاط
+
+588
+00:45:13,660 --> 00:45:20,160
+طبيعي، تمام؟ وفبالتالي هي ما بتسببش حساسية، تمام؟
+
+589
+00:45:20,160 --> 00:45:25,740
+وهي puncture resistant، يعني مقاومة لإنه يصير ثقب
+
+590
+00:45:25,740 --> 00:45:30,820
+فيها، يعني لو الـ needle بدها تنخزها، عشان أنا..يعني
+
+591
+00:45:30,820 --> 00:45:35,700
+لو صار في accident وبدي أنخز نفسي، إذا أنا يعني
+
+592
+00:45:35,700 --> 00:45:40,340
+لابسة nitrile gloves، بيصعب إنه يصير في..أن
+
+593
+00:45:40,340 --> 00:45:44,200
+أنا أنخز نفسي، medical grade، يعني هي مفضّلة في
+
+594
+00:45:44,200 --> 00:45:48,140
+المجال الطبّي، بيستخدموها في العمليات، لونها أزرق وهي
+
+595
+00:45:48,140 --> 00:45:52,670
+بتعطيني درجة حماية أكبر، الـ vinyl gloves، لسه
+
+596
+00:45:52,670 --> 00:45:56,670
+durable، يعني لسه protection، لسه expensive، يعني ما
+
+597
+00:45:56,670 --> 00:46:02,910
+بتتحمّلش كتير، بتعطيني حماية أقل وهي بالتالي أرخص
+
+598
+00:46:02,910 --> 00:46:07,550
+الثمن، مش expensive، تمام؟ هذا بالنسبة لأنواع الـ
+
+599
+00:46:07,550 --> 00:46:10,530
+gloves، تمام؟
+
+600
+00:46:12,170 --> 00:46:19,210
+الـ procedure،  تجربة الـ..الـ..الـ skin puncture
+
+601
+00:46:19,210 --> 00:46:24,470
+أول حاجة، approach the patient in a friendly, calm
+
+602
+00:46:24,470 --> 00:46:28,170
+manner، provide for their comfort as much as
+
+603
+00:46:28,170 --> 00:46:31,810
+possible and gain the patient's cooperation، أول
+
+604
+00:46:31,810 --> 00:46:36,130
+حاجة تعاملي مع المريض بشكل friendly ودود وهادئ،
+
+605
+00:46:36,130 --> 00:46:39,250
+طبعًا ما تكوني aggressive معاه، ما تكوني عنيفة أو
+
+606
+00:46:39,250 --> 00:46:44,930
+يعني معصبة أو متوترة، تمام؟ وبدي أكون مرتاحة له
+
+607
+00:46:44,930 --> 00:46:48,530
+إن شاء الله، ما يريح، ما يتألم كثير، ويعني هي نخزة
+
+608
+00:46:48,530 --> 00:46:53,990
+بسيطة، وأحاول أريحه، يعني لو هو متوتر أخليه يعني هيك
+
+609
+00:46:53,990 --> 00:46:58,870
+يأخذ نفس عميق، يحاول يرتاح، أمسك إيده، أعمل لها
+
+610
+00:46:58,870 --> 00:47:01,670
+rubbing، يعني أفرك إيده، أحاول أدفّي إيده، إذا إيده
+
+611
+00:47:01,670 --> 00:47:07,640
+باردة، فبالتالي هيك أنا بكون عم برّيحه نفسيًا، and gain
+
+612
+00:47:07,640 --> 00:47:10,580
+the patient's cooperation، وبالتالي هيك بكون أيضًا
+
+613
+00:47:10,580 --> 00:47:14,720
+بخلي المريض يكون متعاون معي أكثر، identify
+
+614
+00:47:14,720 --> 00:47:18,060
+the patient correctly، بدي أتعرّف على المريض
+
+667
+00:51:19,260 --> 00:51:22,320
+بتعرفوا إحنا بنمسك الجُنْجُم باستخدام الإبهام والسبابة
+
+668
+00:51:22,320 --> 00:51:26,980
+والسبابة تبعنا غالبا يعني بيكون rigid أو ناشف شوية
+
+669
+00:51:26,980 --> 00:51:33,080
+طبعا فبرضه كمان ببتعد عنه وفال .. ال .. ال ..
+
+670
+00:51:33,080 --> 00:51:36,320
+الإصبع رقم ثلاثة وأربعة هما اللي هي فيهم طراوة
+
+671
+00:51:36,320 --> 00:51:39,840
+وفيهم الـ soft tissue يعني كفيل بأن أنا أعمل نخزة
+
+672
+00:51:39,840 --> 00:51:44,900
+بعيدة عن العظم، الأصبع الصغير هذا الحزين ال .. ال ..
+
+673
+00:51:44,900 --> 00:51:49,180
+ال .. البنصر طبعا صعب أن أنا أعمله نخز لإنه هـ ..
+
+674
+00:51:49,180 --> 00:51:55,660
+هأقضيه، تمام؟ هذا بالنسبة للأصابع، الثالث والرابع
+
+675
+00:51:55,660 --> 00:51:59,460
+ويُفضّل أن أنا أستخدم يا بنات الـ non-dominant hand
+
+676
+00:51:59,800 --> 00:52:04,140
+تمام؟ يُفضّل أن أنا أستخدم الـ non-dominant hand، إيش
+
+677
+00:52:04,140 --> 00:52:07,060
+يعني الـ non-dominant hand؟ يعني هو إذا بكتب بالـ ..
+
+678
+00:52:07,060 --> 00:52:12,180
+بالإيد اليمنى بسحب له من اليسرة، إذا هو أعسر بكتب
+
+679
+00:52:12,180 --> 00:52:16,180
+بالإيد اليسرى بسحب له من اليمنى وهكذا، don't use
+
+680
+00:52:16,180 --> 00:52:20,180
+the tip of the finger or center of finger، تمام؟
+
+681
+00:52:20,180 --> 00:52:26,640
+يُمنع أن أستخدم الـ .. الـ رأس الإصبع يعني أو
+
+682
+00:52:26,640 --> 00:52:29,890
+المركز، طبعا، تمام؟ avoid
+
+683
+00:52:32,940 --> 00:52:37,960
+the side of the finger كمان على الجوانب، تمام؟ يعني
+
+684
+00:52:37,960 --> 00:52:42,160
+ما بِسْ.. ما بِسْ بعمل نخزة على الجوانب لإنه هيك أنا
+
+685
+00:52:42,160 --> 00:52:46,120
+بكون .. يعني جربت من منطقة الأظافر وصعب هذه
+
+686
+00:52:46,120 --> 00:52:49,760
+المنطقة أن أنا أعمل لها نخز، تمام؟ وأيضا في .. على
+
+687
+00:52:49,760 --> 00:52:53,700
+الجوانب في، لسه تشوفها تكون مؤلمة، تمام؟ يعني في
+
+688
+00:52:53,700 --> 00:52:58,580
+كمان أيضا تجمع للأعصاب على الجوانب فهذه بتؤدي إلى
+
+689
+00:52:58,580 --> 00:53:04,330
+أنه يكون الشيء مؤلم، تمام الـ .. الـ second اللي هو الـ
+
+690
+00:53:04,330 --> 00:53:09,390
+.. الـ index، الأصبع السبابة رقم اثنين هو thicker، هو
+
+691
+00:53:09,390 --> 00:53:14,150
+سميك، يعني جلده سميك نفسه فصعب أن أنا أعمله نخزا و
+
+692
+00:53:14,150 --> 00:53:20,330
+أصل يعني لعمق معين وأحصل على كمية الدم الكافية، و
+
+693
+00:53:20,330 --> 00:53:23,630
+الـ fifth finger، الأصبع الصغير الحزين حكينا أنه
+
+694
+00:53:23,630 --> 00:53:28,750
+عنده less soft tissue فبالتالي يعني ممكن أنا أصل
+
+695
+00:53:28,750 --> 00:53:33,150
+للعظم بسهولة وأنا عم بنخزه فبالتالي أسبب له ألم أو
+
+696
+00:53:33,150 --> 00:53:37,030
+damage للبُنْ، avoid puncturing a finger which that
+
+697
+00:53:37,030 --> 00:53:39,950
+is cold or cyanotic, swollen, scarred or covered
+
+698
+00:53:39,950 --> 00:53:43,980
+with rash، يعني الإصبع اللي في إيد لو كانت باردة
+
+699
+00:53:43,980 --> 00:53:47,860
+أو مزرقة، ما .. ما .. ما بنخزها لإنه طبعا هي باردة
+
+700
+00:53:47,860 --> 00:53:50,980
+أو مزرقة، يا إما من الخوف يا إما من التوتر أو من ..
+
+701
+00:53:50,980 --> 00:53:55,220
+من حالة مرضية عنده، يعني مادامها باردة ومزرقة يعني
+
+702
+00:53:55,220 --> 00:53:58,660
+ما فيه blood supply كافٍ فبالتالي يعني أنا لو نخزته
+
+703
+00:53:58,660 --> 00:54:01,600
+مش راح أحصل على كمية دم كافية، يعني بس عم بكون بقلم
+
+704
+00:54:01,600 --> 00:54:05,610
+المريض وخلاص، swollen، الـ .. إذا كان هو .. يعني إصبع
+
+705
+00:54:05,610 --> 00:54:09,730
+منتفخ وأنا أجي أنخزه يعني أنا هيك عم بِسَبِّب له مشكلة
+
+706
+00:54:09,730 --> 00:54:14,050
+إضافية، إذا كان فيه scar أو .. أو .. أو عنده rash
+
+707
+00:54:14,050 --> 00:54:22,230
+rash يعني .. يعني الـ .. الـ .. الـ .. الـ شيء يعني هو
+
+708
+00:54:22,230 --> 00:54:30,750
+الأحمر اللي موجود يعني الـ .. الـ .. يعني مش عارفة
+
+709
+00:54:30,750 --> 00:54:36,970
+أترجمه بالعربي الصراحة، هي الـ rash يعني الـ .. إذا
+
+710
+00:54:36,970 --> 00:54:40,810
+كان، أحيانا بيكون في حساسية وبيكون في احمرار مع
+
+711
+00:54:40,810 --> 00:54:46,930
+حبوب صغيرة وأشياء هذه بيسميها rash، تمام؟ يُفضّل
+
+712
+00:54:46,930 --> 00:54:51,450
+أن أنا ما أستخدمها، تمام؟ إذا كان الإيد فيها rash
+
+713
+00:54:51,450 --> 00:54:55,690
+ما أستخدمها، تمام؟ يعني .. يعني دلّت على وجود
+
+714
+00:54:55,690 --> 00:54:58,070
+حساسية أو infection أو التهابات معينة
+
+715
+00:55:01,400 --> 00:55:06,360
+طبعا إذا أنا بدي أسحب عينة من الـ heel of the foot،
+
+716
+00:55:06,360 --> 00:55:10,120
+من كعب الرجل، طبعا، حكينا الأطفال ممكن أسحب لهم من
+
+717
+00:55:10,120 --> 00:55:10,860
+كعب الرجل
+
+718
+00:55:14,500 --> 00:55:17,900
+when obtaining blood from infant less than one
+
+719
+00:55:17,900 --> 00:55:22,000
+year، إذا كان الطفل أقل من سنة يُفضّل أن أنا آخذ له
+
+720
+00:55:22,000 --> 00:55:25,760
+العينة من كعب رجله، تمام؟ the site chosen should
+
+721
+00:55:25,760 --> 00:55:30,820
+be on the inside medial or outside plantar of the
+
+722
+00:55:30,820 --> 00:55:36,960
+foot، يعني هاي .. هاي الرجل، تمام؟ رجل المريض،
+
+723
+00:55:36,960 --> 00:55:42,270
+خليني أكبّر الـ slide، هي هذه رجل المريض، تمام؟ هذا الـ
+
+724
+00:55:42,270 --> 00:55:47,170
+plantar surface، يعني المنطقة الطرفية من القدم
+
+725
+00:55:47,170 --> 00:55:50,730
+بسمّيها الـ plantar surface، وهذا الـ medial المنطقة
+
+726
+00:55:50,730 --> 00:55:54,750
+الوسطى، يعني المحادية للرجل الثانية، يعني المنطقة
+
+727
+00:55:54,750 --> 00:55:59,030
+الداخلية من الرجل، بسمّيها الـ plant .. الـ medial
+
+728
+00:55:59,030 --> 00:56:05,000
+surface، تمام؟ لو أنا بدي أرسم خط imaginary line
+
+729
+00:56:05,000 --> 00:56:13,500
+تمام، خط وهمي بـ .. برسمه طبعا من منتصف الأصبع
+
+730
+00:56:13,500 --> 00:56:20,840
+الكبير، تمام، إلى كعب الرجل، وأيضا خط آخر من الـ ..
+
+731
+00:56:21,740 --> 00:56:26,080
+المسافة ما بين الإصبع الرابع والخامس اللي هي هذه
+
+732
+00:56:26,080 --> 00:56:32,700
+المسافة، وأيضا برسم خط يتقاطع في الـ .. الـ .. كعب
+
+733
+00:56:32,700 --> 00:56:37,380
+الرجل، تمام؟ أي منطقة في كعب الرجل خارج رأس المثلث
+
+734
+00:56:37,380 --> 00:56:41,940
+بسمّيها هي المنطقة اللي يعني ممكن أنا أسحب منها عينة
+
+735
+00:56:41,940 --> 00:56:48,240
+الدم، إذا أول ما بمسك رجل الطفل لأقل من سنة برسم خط
+
+736
+00:56:48,240 --> 00:56:54,900
+وهمي بنظري يمتد من نصف الأصبع الكبير إلى إياه إلى
+
+737
+00:56:54,900 --> 00:56:59,400
+من نصف الأصبع الكبير إلى كعب الرجل، ومن أو الـ line
+
+738
+00:56:59,400 --> 00:57:04,240
+from between the fourth and the fifth toe، وخط أيضا
+
+739
+00:57:04,240 --> 00:57:07,780
+من المسافة ما بين الأصبع الرابع والخامس إلى كعب
+
+740
+00:57:07,780 --> 00:57:10,380
+الرجل، تمام؟
+
+741
+00:57:11,190 --> 00:57:13,910
+المنطقة هذه اللي في كعب الرجل اللي في رأس المثلث
+
+742
+00:57:13,910 --> 00:57:17,150
+بستثنيها، إذا أي منطقة خارج رأس المثلث هي
+
+743
+00:57:17,150 --> 00:57:21,370
+المنطقة اللي acceptable for puncture، إذا the area
+
+744
+00:57:21,370 --> 00:57:24,910
+outside of these two lines، المنطقة اللي خارج
+
+745
+00:57:24,910 --> 00:57:31,530
+تقاطع الخطين هدول، يعني خارج رأس المثلث،
+
+746
+00:57:31,530 --> 00:57:35,730
+هذه المنطقة هي acceptable for the puncture، تمام
+
+747
+00:57:36,910 --> 00:57:41,410
+طيب، هلّأ الـ .. الـ puncture depth، عمق الـ puncture،
+
+748
+00:57:41,410 --> 00:57:48,780
+تمام؟ يعني أنا بدي .. قد ايش بدي أفوت عمق في الـ .. الـ
+
+749
+00:57:48,780 --> 00:57:53,080
+skin اللي أنا عم بنخزه للمريض، for finger .. for
+
+750
+00:57:53,080 --> 00:57:59,400
+finger stick، لو أنا ماخدة العينة من الإصبع، تمام
+
+751
+00:57:59,400 --> 00:58:04,400
+not deeper than 3.1 millimeter، يعني ممنوع أفوت
+
+752
+00:58:04,400 --> 00:58:09,730
+أكثر من 3.1 millimeter، تمام؟ طيب الـ distance
+
+753
+00:58:09,730 --> 00:58:14,350
+between the skin surface and bone، دائما المسافة ما
+
+754
+00:58:14,350 --> 00:58:18,630
+بين الـ .. الـ .. الـ skin surface، ما بين الجلد و
+
+755
+00:58:18,630 --> 00:58:25,730
+العظم بتتراوح ما بين 3.1 إلى 10.9 millimeter، تمام؟
+
+756
+00:58:25,730 --> 00:58:29,970
+إذا أنا لازم ما أصل 3.1 لإني ممكن هيك أكون وصلت
+
+757
+00:58:29,970 --> 00:58:34,730
+للعظم، لازم أكون وصلت قبل الـ 3.1، يعني أنا أكون
+
+758
+00:58:34,730 --> 00:58:41,610
+ما أكونش فوتت أعمق من 3.1، تمام؟ طيب بالنسبة لـ
+
+759
+00:58:41,610 --> 00:58:48,250
+الأطفال اللي يعني عمرهم ست شهور، الطفل هذا المسافة
+
+760
+00:58:48,250 --> 00:58:52,950
+ما بين الـ skin والعظم بتتراوح ما بين واحد ونصف
+
+761
+00:58:53,840 --> 00:58:58,220
+millimeter إلى اثنين وأربعة millimeter، تمام؟ فبالتالي
+
+762
+00:58:58,220 --> 00:59:02,800
+يُفضّل إيش؟ عشان هيك الـ finger ما بستخدمه لهذول
+
+763
+00:59:02,800 --> 00:59:06,680
+الأطفال، بُفَضِّل أن أنا أستخدم لهم heel puncture، تمام
+
+764
+00:59:06,680 --> 00:59:11,390
+بستخدم لهم أو بعمل لهم heel puncture، تمام؟ إذا بالنسبة
+
+765
+00:59:11,390 --> 00:59:14,450
+لـ finger stick عند الناس الـ adult أو الناس اللي
+
+766
+00:59:14,450 --> 00:59:19,070
+مسموح لي أنا آخذ منهم من الأصبع، مش لازم أتجاوز الـ 3
+
+767
+00:59:19,070 --> 00:59:24,430
+.1 mm، عمق النخزة، علشان لإني المسافة ما بين الـ skin
+
+768
+00:59:24,430 --> 00:59:28,920
+والعظم بتتراوح ما بين 3.1 إلى عشرة point تسعة
+
+769
+00:59:28,920 --> 00:59:33,840
+millimeter، عند الناس، طبعا، يعني أنا مادام مش لازم
+
+770
+00:59:33,840 --> 00:59:36,600
+أصل عمق ثلاثة point واحد لإني في خاطر أكون وصلت
+
+771
+00:59:36,600 --> 00:59:39,940
+للعظام، لازم أكون وصلت .. العمق يكون قبل الثلاثة
+
+772
+00:59:39,940 --> 00:59:46,960
+point واحد، تمام؟ for heel puncture، عمق الـ puncture
+
+773
+00:59:46,960 --> 00:59:53,340
+تمام، في أي وقت لازم ما يكونش أكثر من 2.4، يعني في الـ
+
+774
+00:59:53,340 --> 00:59:57,240
+general، في الشكل .. بالشكل العام مش لازم يكون أعمق
+
+775
+00:59:57,240 --> 01:00:02,820
+من 2.4 millimeter، عشان أـ .. يعني أتجنّب أن أنا أعمل
+
+776
+01:00:02,820 --> 01:00:06,540
+damage للـ bone، because the blood supply for the
+
+777
+01:00:06,540 --> 01:00:10,580
+infant's heel is located between one point، يعني
+
+778
+01:00:10,580 --> 01:00:14,440
+الدم موجود، يعني الأوعية الدموية، الشعيرات الدموية
+
+779
+01:00:14,440 --> 01:00:20,360
+موجودة على بعد من point ثلاثة خمسة إلى واحد وستة
+
+780
+01:00:20,360 --> 01:00:24,980
+point millimeter، واحد point ستة millimeter، تمام؟
+
+781
+01:00:24,980 --> 01:00:29,360
+تحت الـ skin، فلازم الضربة تبعتي ما تكونش أعمق من
+
+782
+01:00:29,360 --> 01:00:32,870
+واحد point ستة، يعني تكون في .. في العمق ما بين
+
+783
+01:00:32,870 --> 01:00:37,150
+point ثلاثة خمسة لواحد point ستة، يعني في العمق
+
+784
+01:00:37,150 --> 01:00:40,050
+اللي موجودة فيه الأوعية الدموية، أنا مش لازم أصل
+
+785
+01:00:40,050 --> 01:00:43,490
+أعمق لإني هيك أكون .. بكون عم بقلم المريض، ممكن
+
+786
+01:00:43,490 --> 01:00:48,050
+أوصل للعظم، ممكن أوصل للـ nerve تبعته فبالتالي أَقْلِمُه
+
+787
+01:00:48,050 --> 01:00:53,840
+و .. أو أسبب له damage، يعني فعلًا فاضي، يعني أنا بس
+
+788
+01:00:53,840 --> 01:00:57,640
+محتاجة ضربة قوية، عميقة enough .. enough deep، تمام؟
+
+789
+01:00:57,640 --> 01:01:02,880
+بحيث إنه يعني يتمكّنني أن أنا أسحب عينة الدم تبعتها، طيب
+
+790
+01:01:02,880 --> 01:01:05,620
+performance of skin puncture، مجرد ما أنا عملت
+
+791
+01:01:05,620 --> 01:01:09,040
+التحضير هذه وعرفت من وين بدي أسحب العينة، تمام؟
+
+792
+01:01:09,040 --> 01:01:12,040
+هلّأ بدي أعمل الـ .. الـ .. الـ performance of skin
+
+793
+01:01:12,040 --> 01:01:15,380
+puncture، the puncture site should be .. shouldn't
+
+794
+01:01:15,380 --> 01:01:18,520
+be cold، طبعا مش لازم يكون بارد ولا cyanotic ولا
+
+795
+01:01:18,520 --> 01:01:25,220
+swollen ولا فيه rash الـ .. عليه يعني احمرار أو
+
+796
+01:01:25,220 --> 01:01:32,020
+حساسية أو انتفاخ أو أي شيء، تمام؟ must be warmed
+
+797
+01:01:32,020 --> 01:01:36,460
+either by rubbing the side, hanging the hand or
+
+798
+01:01:36,460 --> 01:01:43,000
+applying hot gauze in venture side، هلّأ شوفوا معايا
+
+799
+01:01:43,000 --> 01:01:47,340
+طبعا مش لازم المكان يكون بارد وإذا كان بارد أنا
+
+800
+01:01:47,340 --> 01:01:50,000
+لازم أدفيه إما من خلال أن أنا أمسك إيده وأفركها
+
+801
+01:01:50,000 --> 01:01:54,060
+بين إيديا أو أخليها أعملها hanging hand، إيش يعني
+
+802
+01:01:54,060 --> 01:01:58,430
+hanging hand؟ طبعا، هلّأ أنتِ جالسة على كرسي المكتب،
+
+803
+01:01:58,430 --> 01:02:02,470
+إيدك على مستوى، لو جربت تنزلي إيدك لتحت .. لتحت،
+
+804
+01:02:02,470 --> 01:02:06,710
+يعني باتجاه .. يعني بحيث إنه أصابعك تكون مشيرة إلى
+
+805
+01:02:06,710 --> 01:02:10,030
+الأسفل، إلى الأرض، طبعا، هتلاحظ إنه الأوعية
+
+806
+01:02:10,030 --> 01:02:13,810
+الدموية تبعتك اللي في إيدك، طلعي على الشرايين اللي
+
+807
+01:02:13,810 --> 01:02:18,090
+في إيدك، طبعا، هتلاحظ إنها هي بَرَزَتْ، ارفعي إيدك مرة
+
+808
+01:02:18,090 --> 01:02:23,510
+ثانية، بتلاحظ إنه اختفى البروز هذا، تمام؟ فارجعي
+
+809
+01:02:23,510 --> 01:02:27,490
+نزّليها مرة ثانية، معناه إذا أنتِ نزلتي إيدك أدنى من
+
+810
+01:02:27,490 --> 01:02:31,130
+مستواكي، معناه أنتِ سمحتي للدم أنه يتضفّق في إيدها،
+
+811
+01:02:31,130 --> 01:02:35,670
+على طول بتدفعه، تمام؟ أو من خلال أن أنا آخذ شاش
+
+812
+01:02:35,670 --> 01:02:41,350
+دافئ أو شاش ساخن و يعني hot gauze وأحطه على مكان
+
+813
+01:02:41,350 --> 01:02:46,650
+الـ puncture عشان أدفيه، طيب use a sterile lancet، إذا
+
+814
+01:02:46,650 --> 01:02:49,950
+الشفرة أو الأداة اللي أنا بستخدم فيها بسحب فيها من
+
+815
+01:02:49,950 --> 01:02:54,470
+الـ skin puncture، اسمها lancet، تمام؟ إيش اسمها؟
+
+816
+01:02:54,470 --> 01:03:00,650
+lancet، make، طبعا لازم أنا أعمل puncture، ضربة أو
+
+817
+01:03:00,650 --> 01:03:05,090
+ثقب من خلال .. من خلال إيش؟ لازم إيه لها صفات، هاي
+
+818
+01:03:05,090 --> 01:03:08,890
+الضربة اللي أنا بقدر أعملها؟ تكون strong، قوية و
+
+819
+01:03:08,890 --> 01:03:13,190
+fast and deep skin puncture، إذا لازم تكون strong و
+
+820
+01:03:13,190 --> 01:03:17,550
+fast و deep، ما بِينْفَعْ أنا أكون هيك يعني عم .. عم بحرّك
+
+821
+01:03:17,550 --> 01:03:22,530
+إيدي ببطء و .. عشان أجي أنخز المريض أو إنه يكون ..
+
+822
+01:03:22,530 --> 01:03:25,930
+تكون يدوب خفيفة الضربة، لأ، يعني أنا بكون ممكن ..
+
+823
+01:03:25,930 --> 01:03:29,250
+إذا خفيفة، يعني معناها أنا بس خدشت الـ skin، ما فوتش
+
+824
+01:03:29,250 --> 01:03:34,390
+deep enough، تمام؟ فبالتالي لازم strong, fast, deep،
+
+825
+01:03:34,390 --> 01:03:39,370
+تمام؟ وأيضا لازم تكون من خصائصها إنها تكون
+
+826
+01:03:39,370 --> 01:03:43,770
+إتجاهها يا بنات perpendicular to the ridges of the
+
+827
+01:03:43,770 --> 01:03:49,030
+fingerprint، وعمودية على خطوط البصمة، لحظة، طلع على
+
+828
+01:03:49,030 --> 01:03:55,560
+أصابعك، مخططين بشكل دائري، تمام؟ لازم الـ .. الضربة
+
+829
+01:03:55,560 --> 01:04:02,100
+تبعتي تكون عمودية على خطوط البصمة، وإن شاء الله بس
+
+830
+01:04:02,100 --> 01:04:06,100
+نرجع للمختبر ونجرّب الـ skin puncture، راح أورجيكم
+
+831
+01:04:06,100 --> 01:04:11,420
+إيش معنى كلمة عمودية على خطوط البصمة، تمام؟ طيب، هذا
+
+832
+01:04:11,420 --> 01:04:15,040
+.. ده أنا .. طبعا ليش أنا بديها عمودية؟ لإنه لو
+
+833
+01:04:15,040 --> 01:04:18,760
+أنا عملت الـ .. الـ .. الجرح أو الـ .. الـ .. الثقوب
+
+834
+01:04:18,760 --> 01:04:22,660
+أو الـ skin puncture موازية لخطوط البصمة راح يسيل
+
+835
+01:04:22,
+
+889
+01:08:28,550 --> 01:08:32,280
+قمت فيه والقطن اللي مسحت فيه أول قطرة ال...ال
+
+890
+01:08:32,280 --> 01:08:35,600
+Lancet اللي استخدمتها لإحداث ال...النخزة أو
+
+891
+01:08:35,600 --> 01:08:40,780
+الثقوب، وهذه كلها الأدوات بتخلص منها properly في
+
+892
+01:08:40,780 --> 01:08:44,720
+الـ container الخاص فيها. مثلاً الـ Lancet عبارة عن
+
+893
+01:08:44,720 --> 01:08:48,660
+أداة حادة بستخدم... بأُطْلِفها في الـ sharp box. القطن
+
+894
+01:08:48,660 --> 01:08:51,880
+الملوث بالدم، إذا بحطه في السلة الموجود...م...م...
+
+895
+01:08:51,880 --> 01:08:55,760
+محطوط عليها علامة biohazard. القطن غير الملوث بالدم
+
+896
+01:08:55,760 --> 01:09:00,060
+بستخ... بأُطْلِفُه في السلة العادية، تمام؟ الورقة
+
+897
+01:09:00,060 --> 01:09:04,020
+المغلفة لا، الـ Lancet بلُفها بالورقة العادية بالسلة
+
+898
+01:09:04,020 --> 01:09:09,140
+العادية. فبالتالي، إذا كل شيء لازم أخلص منه بالـ
+
+899
+01:09:09,140 --> 01:09:14,020
+container المناسب له، بالوعاء المناسب له،  بالـ label
+
+900
+01:09:14,020 --> 01:09:18,680
+الـ appropriated tubes at the patient bedside. طبعاً
+
+901
+01:09:18,680 --> 01:09:22,680
+لازم أنا العينة بتاعتي أعمل لها labeling وأنا
+
+902
+01:09:22,680 --> 01:09:27,750
+بأستَنى جنب المريض، مش يعني إنه خلاص يلا أروح و
+
+903
+01:09:27,750 --> 01:09:30,270
+آخِد اللي بعده، وبعدين أعمل labeling. ما أنا
+
+904
+01:09:30,270 --> 01:09:34,930
+بنسى، تمام؟ أو ممكن أخطئ، فلذلك ما أعتمد على
+
+905
+01:09:34,930 --> 01:09:40,470
+ذاكرتي. أفضل ذاكرة هي الذاكرة المكتوبة، تمام؟ و
+
+906
+01:09:40,470 --> 01:09:45,070
+أكتب مباشرة المعلومات، أعمل labeling للعينة
+
+907
+01:09:45,070 --> 01:09:51,170
+بالمعلومات اللازمة، يعني الاسم، اسمه الأول وعائلته
+
+908
+01:09:51,170 --> 01:09:55,230
+و الـ initial of middle name، التاريخ، الميلاد، الـ
+
+909
+01:09:55,230 --> 01:09:59,490
+gender، الـ... طبعاً مثلاً مهم يا بنات كمان أعمل
+
+910
+01:09:59,490 --> 01:10:05,820
+labeling للـ gender. طبعاً مثلاً في شخص اسمه ضياء، طبعاً
+
+911
+01:10:05,820 --> 01:10:09,660
+أنا مش عارفة هو ضياء بنت ولا ولد، تمام؟ لازم يكون
+
+912
+01:10:09,660 --> 01:10:13,580
+يعني يميز إنه هو male أو female، خصوصاً مع الأسماء
+
+913
+01:10:13,580 --> 01:10:17,280
+اللي تصلح إنه يكون لـ male أو female، زي ضياء، نور،
+
+914
+01:10:17,280 --> 01:10:21,760
+إسلام، زي أشياء...الأسماء هذه، مهم
+
+915
+01:10:21,760 --> 01:10:25,100
+إن أنا يعني أو مع كل الأسماء، مهم إن أنا أميز الـ
+
+916
+01:10:25,100 --> 01:10:28,720
+gender of patient. delivers specimen promptly to the
+
+917
+01:10:28,720 --> 01:10:33,900
+laboratory فوراً. لازم أنا أسلم العينة للمختبر
+
+918
+01:10:33,900 --> 01:10:38,920
+لإجراء الفحوصات اللازمة. إذا بحدد الإصبع المراد
+
+919
+01:10:38,920 --> 01:10:43,560
+إجراء skin puncture عليه، تمام؟ لازم يكون الثالث أو
+
+920
+01:10:43,560 --> 01:10:47,100
+الرابع. هي الأول، الإبهام، الثاني هي هنا،
+
+921
+01:10:47,100 --> 01:10:51,880
+الثالث، الوسطى، وهي الرابع والأخير، الخامس طبعاً هو
+
+922
+01:10:51,880 --> 01:10:57,040
+الوسطى والخنصر هم اللي بأستخدمهم، أو يعني هو
+
+923
+01:10:57,040 --> 01:11:00,560
+الثالث والرابع، يعني رقم ثلاثة، الوسطى واللي بعده،
+
+924
+01:11:00,560 --> 01:11:05,700
+هذول اللي مناسبين. طبعاً بأحضّر الأدوات بتاعتي، بأفتح الـ
+
+925
+01:11:05,700 --> 01:11:10,680
+Lancet طبعاً، وبُعْقِمها يا بنات، بعقم المكان بالكحول،
+
+926
+01:11:10,680 --> 01:11:13,520
+بفتح الـ Lancet بتاعتي، الـ Lancet زي ما أنتو شايفين
+
+927
+01:11:13,520 --> 01:11:18,100
+شفرة مدببة الطرف، طبعاً إلها طرف مدبب فأنا بفو...
+
+928
+01:11:18,100 --> 01:11:22,740
+بأعمل ضربة بحيث إن هذه الضربة تكون strong, fast,
+
+929
+01:11:23,420 --> 01:11:26,700
+deep and perpendicular. الأربع خواص، طبيعتها اللي
+
+930
+01:11:26,700 --> 01:11:32,090
+بتيجي أسألكم عليها بالامتحان. تمام؟ بعدين بمسح، بتخلص
+
+931
+01:11:32,090 --> 01:11:35,450
+من أول قطرة الدم، بعدين ببدأ من هنا من عند بداية
+
+932
+01:11:35,450 --> 01:11:40,090
+الإصبع بأعمل gentle massage، المساج خفيف جداً، ولما
+
+933
+01:11:40,090 --> 01:11:44,310
+أصل لمنطقة النخزة بضغط ضغطة، تمام؟ مجرد ما أضغط ضغطة
+
+934
+01:11:44,310 --> 01:11:48,910
+...ضغطة تمام، بيبدأ تطلع عندي تتشكل قطرة دائرية،
+
+935
+01:11:48,910 --> 01:11:53,790
+بجمعها في الـ tube المخصص لجمع العينات، وبعد هيك
+
+936
+01:11:53,790 --> 01:11:57,270
+إيش بس؟ برسل ال... بأعمل labeling للعينة مباشرة، و
+
+937
+01:11:57,270 --> 01:12:01,650
+برسلها للمختبر. بأعطي المريض قطنة أو شاش أو قطعة
+
+938
+01:12:01,650 --> 01:12:06,010
+معقمة إنه هو يضغط على المكان، الجرح، لحين التوقف
+
+939
+01:12:06,010 --> 01:12:09,090
+النزيف، تمام؟ أنتو شايفين القطرة كيف...القطرة كيف
+
+940
+01:12:09,090 --> 01:12:13,290
+اتكونت دائرية؟ لأن أنا فوت عمودية على الـ ridges،
+
+941
+01:12:13,290 --> 01:12:18,490
+تمام؟ وهأحكي لكم إيش يعني عمودية في داخل المختبر إن
+
+942
+01:12:18,490 --> 01:12:21,670
+شاء الله، تمام؟
+
+943
+01:12:24,170 --> 01:12:29,030
+طيب، خلينا بس ال...ال...ناخد المناقشة أو الجزء
+
+944
+01:12:29,030 --> 01:12:34,730
+المتعلق بال...السحب
+
+945
+01:12:34,730 --> 01:12:39,210
+...العينة الدم من الـ skin. طبعاً يا بنات العينة الـ
+
+946
+01:12:39,210 --> 01:12:42,190
+skin puncture، أو الدم اللي جاي من الـ skin puncture،
+
+947
+01:12:42,190 --> 01:12:47,290
+هو مش دم وريدي ولا شرياني، هو عبارة عن mix من دم
+
+948
+01:12:47,290 --> 01:12:51,190
+شرياني ووريدي
+
+949
+01:12:51,190 --> 01:12:55,130
+ومن capillary tubes، تمام؟ ويعني حتى كمان من
+
+950
+01:12:55,130 --> 01:12:59,890
+الشعيرات الدموية. فبالتالي، التركيز داخل هذا الدم
+
+951
+01:12:59,890 --> 01:13:03,590
+بيكون مختلفة. مثلاً تركيز ال...ال...الـ analytes أو
+
+952
+01:13:03,590 --> 01:13:07,830
+المواد المراد قياسها، أو ال...ال...ال...الأشياء
+
+953
+01:13:07,830 --> 01:13:11,150
+المراد قياسها، سواء كانت أنا ببدأ أقيس بروتينات،
+
+954
+01:13:11,150 --> 01:13:15,060
+هرمونات، glucose، دهون، أي شيء بدي أقيصه في...في
+
+955
+01:13:15,060 --> 01:13:19,300
+العينة هذه، تمام؟ دي بأجري له فحص، لازم... بيكون مختلف
+
+956
+01:13:19,300 --> 01:13:24,380
+طبعاً تركيزه داخلها عن تركيزه في الوريد، لأنه هذا
+
+957
+01:13:24,380 --> 01:13:28,120
+عبارة عن mixture، مزيج من الدم الوريدي والشرياني
+
+958
+01:13:28,120 --> 01:13:34,500
+وحتى الشعيرات الدموية. فبالتالي، لذلك لازم أنا أ
+
+959
+01:13:34,500 --> 01:13:41,470
+mention، أكتب في ال...الـ report، في النتيجة، في
+
+960
+01:13:41,470 --> 01:13:46,030
+التقرير اللي بيطلع من المختبر، النتيجة، أحكي إنه هذه
+
+961
+01:13:46,030 --> 01:13:52,930
+النتيجة اللي، الفحص، أجري على عينة was obtained from
+
+962
+01:13:52,930 --> 01:13:57,890
+skin puncture، تمام؟ إذا تركيز مختلفة بتكون، والـ
+
+963
+01:13:57,890 --> 01:14:01,860
+difference between skin puncture blood and arterial or
+
+964
+01:14:01,860 --> 01:14:05,960
+venous. يعني بتركيز مختلفة في هذا الدم اللي جاي من الـ
+
+965
+01:14:05,960 --> 01:14:09,260
+skin puncture عن التركيز اللي موجودة في الدم
+
+966
+01:14:09,260 --> 01:14:16,120
+الوريدي أو الشرياني. فبالتالي، لازم إنه طبعاً في...في
+
+967
+01:14:16,120 --> 01:14:20,340
+الـ test report، في التقرير، should indicate، يعني
+
+968
+01:14:20,340 --> 01:14:25,680
+أشير إلى إنه العينة الدم هذه حصلنا عليها من skin
+
+969
+01:14:25,680 --> 01:14:29,060
+puncture، عشان الدكتور لما يقيم وضع المريض ياخد
+
+970
+01:14:29,060 --> 01:14:32,520
+بعين الاعتبار إنه العينة هي skin puncture، يعني
+
+971
+01:14:32,520 --> 01:14:36,380
+التركيز مختلفة شوية. excessive massaging، لو أنا بدي
+
+972
+01:14:36,380 --> 01:14:40,640
+أعمل الـ massage القوي، إيش حكيناه أول حاجة؟ بكون عم
+
+973
+01:14:40,640 --> 01:14:44,020
+بأعمل squeeze، تعصير لـ finger أو الـ foot أو اللي
+
+974
+01:14:44,020 --> 01:14:48,080
+هو المكان لـ skin puncture. فبالتالي، بكون بطلع عصارة
+
+975
+01:14:48,080 --> 01:14:51,820
+الخلايا، وبالتالي بخفف الدم، بيصير عندي diluted
+
+976
+01:14:51,820 --> 01:14:58,050
+blood، أو أحياناً ممكن يسبب تجلط للدم سريع، وبالتالي
+
+977
+01:14:58,050 --> 01:15:01,250
+توقف خروج الدم. يعني أنا ما بأحصل على الـ volume
+
+978
+01:15:01,250 --> 01:15:05,910
+المناسب، يا بأحصل على الدم مخفف، فبالتالي مش لازم أنا
+
+979
+01:15:05,910 --> 01:15:09,570
+أعمل excessive massaging، لأ، gentle massaging،
+
+980
+01:15:09,570 --> 01:15:17,870
+وبالتالي إنه يعني بيكون هيك مناسب لاستمرارية، ضمن
+
+981
+01:15:17,870 --> 01:15:22,130
+استمرارية إنه خروج الدم، وإنه أنا بأحصل على دم مش
+
+982
+01:15:22,460 --> 01:15:26,380
+مخفف. Since deep puncture is not any more
+
+983
+01:15:26,380 --> 01:15:30,400
+painful than superficial puncture, it's best to go
+
+984
+01:15:30,400 --> 01:15:34,000
+deep enough the first time, thereby avoid
+
+985
+01:15:34,000 --> 01:15:37,560
+puncturing the patient a second time. يعني الـ deep
+
+986
+01:15:37,560 --> 01:15:41,560
+puncture بتكونش مؤلمة زي الـ superficial، فبالتالي
+
+987
+01:15:41,560 --> 01:15:45,980
+أنا لازم أُجَهِّز وأعمل puncture، أعمل ضربة قوية، و
+
+988
+01:15:45,980 --> 01:15:51,120
+تكون عميقة enough، تمام؟ عشان أتجَنّب إن أنا أنخز
+
+989
+01:15:51,120 --> 01:15:53,700
+المريض مرة ثانية. لو طلعت superficial، بدي أرجع
+
+990
+01:15:53,700 --> 01:15:57,160
+أنخز مرة ثانية، وهذا non-ethical، مش أخلاقي، إن أنا
+
+991
+01:15:57,160 --> 01:16:01,290
+أضل أجرب على المريض، يعني أنا بكون عم بأذيّه. excess
+
+992
+01:16:01,290 --> 01:16:07,110
+crying، إذا كان المريض هذا متوتر، ويعني ببكي، إذا
+
+993
+01:16:07,110 --> 01:16:12,170
+كان، خصوصاً طفل، هذا راح يأثر على نتائج المختبر، يعني
+
+994
+01:16:12,170 --> 01:16:15,170
+مثلاً ممكن يرفع الـ white pieces، نتيجة الـ stress،
+
+995
+01:16:15,170 --> 01:16:18,050
+يعني ممكن الـ white blood cell count يكون يصير له
+
+996
+01:16:18,050 --> 01:16:23,830
+increase، falsely increase، فيعني يزيد وهو زيادة
+
+997
+01:16:23,830 --> 01:16:28,780
+خاطئة، يعني إنه بس نتيجة التوتر. فبالتالي، أنا الأفضل
+
+998
+01:16:28,780 --> 01:16:32,940
+إن هذا المريض أستنى عليه نص ساعة، على ساعة، بعد ما
+
+999
+01:16:32,940 --> 01:16:37,720
+يخلص يعني موجة البكاء اللي هو فيها، أحاول أهدي،
+
+1000
+01:16:37,720 --> 01:16:40,140
+أستنى عليه من نص ساعة إلى ساعة، وبعدين أسحب له
+
+1001
+01:16:40,140 --> 01:16:45,240
+العينة. طبعاً الـ thumb، وال...وال...يعني
+
+1002
+01:16:45,240 --> 01:16:49,410
+الإصبع الكبير، ال...الإبهام والإصبع الكبير بالرجل،
+
+1003
+01:16:49,410 --> 01:16:53,150
+والـ ear loop، اللي هي حلمة ال...ال...الأذن، أو
+
+1004
+01:16:53,150 --> 01:16:56,910
+يعني طرف...طرف الأذن، المكان ما بنحط ال...ال...
+
+1005
+01:16:56,910 --> 01:17:01,230
+الحلق، يعني هذا طبعاً صعب يا بنات نعمله skin
+
+1006
+01:17:01,230 --> 01:17:05,070
+puncture، ممنوع، يعني ما بأستخدمه، ما...يعني ما بأعمله
+
+1007
+01:17:05,070 --> 01:17:09,050
+skin puncture وما بأخذ منه عينة الدم. يُفَضَّل أني ما آخذ
+
+1008
+01:17:09,050 --> 01:17:12,370
+منه من هذا المكان. Misidentification of the patient،
+
+1009
+01:17:13,540 --> 01:17:17,080
+إذا أنا ما اتعرفت على المريض بشكل صحيح، معناها راح
+
+1010
+01:17:17,080 --> 01:17:22,120
+يصير في عندي طبعاً سلسلة من الـ errors، series of
+
+1011
+01:17:22,120 --> 01:17:26,040
+errors. ما اتعرفت على ال...ال...المريض بشكل صحيح،
+
+1012
+01:17:26,040 --> 01:17:29,500
+إذا أنا سحبت العينة الخطأ من المريض الخطأ، وحصلت
+
+1013
+01:17:29,500 --> 01:17:33,780
+على نتائج خاطئة للمريض، فبالتالي، ما هذا معناه تشخيص
+
+1014
+01:17:33,780 --> 01:17:38,160
+خاطئ، إذا علاج خاطئ، إذا هذا سلسلة من الـ errors،
+
+1015
+01:17:38,160 --> 01:17:43,510
+series of errors، تمام؟ طيب، هو ممكن يكون وقعها
+
+1016
+01:17:43,510 --> 01:17:48,490
+disastrous، أو implication على المريض، يعني...
+
+1017
+01:17:48,490 --> 01:17:54,890
+يعني تأثيرها أو وقعها  كارثي على المريض. beta-
+
+1018
+01:17:54,890 --> 01:17:59,330
+diene...beta-diene أو iodine، طبعاً shouldn't be
+
+1019
+01:17:59,330 --> 01:18:02,110
+used routinely to clean the pleptomy area. يعني أنا
+
+1020
+01:18:02,110 --> 01:18:11,210
+مش لازم أعقم المكان ب...بـ iodine، بمحلول iodine، طبعاً
+
+1021
+01:18:11,210 --> 01:18:15,970
+ليش؟ لإنه يُطْلِق ال...الـ contamination ب...يعني...
+
+1022
+01:18:15,970 --> 01:18:20,410
+ب...يعني إنه بيجي beta-diene أو iodine على مكان
+
+1023
+01:18:20,410 --> 01:18:26,190
+الـ skin puncture، ممكن طبعاً يلوث العينة. يعني لما
+
+1024
+01:18:26,190 --> 01:18:28,950
+أجي أنا أسحب العينة، أسحب العينة وفيها بقايا هذا
+
+1025
+01:18:28,950 --> 01:18:33,380
+الـ iodine، فبالتالي يسبب لي some error test result،
+
+1026
+01:18:33,380 --> 01:18:36,780
+يعني مثلاً ارتفاع في الـ potassium، ارتفاع في الـ uric
+
+1027
+01:18:36,780 --> 01:18:39,600
+acid، ارتفاع في الـ phosphorus result، مثلاً أجي أحكم
+
+1028
+01:18:39,600 --> 01:18:42,700
+على المريض، مثلاً والله عنده uric acid عالي، يعني
+
+1029
+01:18:42,700 --> 01:18:46,260
+مثلاً عنده مشكلة في، مشاكل في وظائف الكبد ووظائف
+
+1030
+01:18:46,260 --> 01:18:50,240
+الكلى. أبدأ أوجه المريض إنه هو مثلاً ممكن يكون عنده
+
+1031
+01:18:50,240 --> 01:18:54,040
+مشاكل في الكلى، أو kidney failure أو كذا مصيبة، تمام؟
+
+1032
+01:18:54,040 --> 01:18:58,560
+نتيجة خلط بسيط في جمع العينة، ممكن يعني ي...أوجه
+
+1033
+01:18:58,560 --> 01:19:01,700
+المريض أو يعني إن أنا يصير في تشخيص خاطئ للمريض،
+
+1034
+01:19:01,700 --> 01:19:05,880
+وبالتالي يعني أُعْرِضُه لإنه يتعرض لأدوية أو علاجات
+
+1035
+01:19:05,880 --> 01:19:10,040
+ممكن تكون خاطئة عليه. skin must be dried، لازم إنه
+
+1036
+01:19:10,040 --> 01:19:13,220
+أنا لما أجي أسحب من العينة...أسحب عينة من المكان،
+
+1037
+01:19:15,150 --> 01:19:18,910
+سواء كان ال...ال...الإصبع أو الـ heel، لازم يكون
+
+1038
+01:19:18,910 --> 01:19:23,110
+جاف تماماً، يعني بقايا الكحول اتبخرت، أو إن أنا إذا
+
+1039
+01:19:23,110 --> 01:19:26,950
+كان استخدمت...يعني أنا استخدمت كحول كثير، أستخدم الـ
+
+1040
+01:19:26,950 --> 01:19:31,750
+...ال...قطن، يعني شاش أو قطن معقم، حتى أمسح بقايا
+
+1041
+01:19:31,750 --> 01:19:36,110
+هذا الكحول. ليش؟ لإنه مجرد يا بنات ما أنا أجي أنخز،
+
+1042
+01:19:36,110 --> 01:19:40,130
+يطلع الدم...يطلع الدم...التقاء الدم مع الكحول،
+
+1043
+01:19:40,130 --> 01:19:44,790
+بيصير في عندي hemolysis of blood، هيتكسر الدم بفعل
+
+1044
+01:19:44,790 --> 01:19:48,460
+التقاء أو الـ mixing تبعه مع الكحول. بيصير فيه
+
+1045
+01:19:48,460 --> 01:19:54,920
+hemolysis، يعني تكسر للدم، تمام؟ هذا طبعاً خطأ، يعني،
+
+1046
+01:19:54,920 --> 01:19:58,780
+فإذا أنا حصلت على دم متكسر، خلايا الدم متكسرة،
+
+1047
+01:19:58,780 --> 01:20:01,520
+فبالتالي أنا مش راح أقدر أعدها، في تحاليل كثيرة مش
+
+1048
+01:20:01,520 --> 01:20:06,490
+راح أقدر أجريها على الـ hemolyzed blood، تمام؟ طيب،
+
+1049
+01:20:06,490 --> 01:20:12,330
+لو أنا خلّيت...طبعاً استخدمت كحول كثير، وخلّيته
+
+1050
+01:20:12,330 --> 01:20:15,670
+فترة...إستَنيت عليه فترة لما يجف...إيش مع...إيش
+
+1051
+01:20:15,670 --> 01:20:21,750
+رح يصير؟ طبعاً يا بنات الكحول لما بيتبخر وبيطير،
+
+1052
+01:20:21,750 --> 01:20:26,470
+بيصير معاه...يعني بيسحب معاه سائل، ماء، بيسحب
+
+1053
+01:20:26,470 --> 01:20:29,730
+معاه ماء، يعني أنتِ...حاليا حط الكحول على إيديكِ والـ
+
+1054
+01:20:29,730 --> 01:20:33,770
+سائل هيتبخر، بتحسي ببرودة، البرودة هذه ناتجة
+
+1055
+01:20:33,770 --> 01:20:36,710
+نتيجة تبخر الماء اللي موجود في خلايانا أو بين
+
+1056
+01:20:36,71
\ No newline at end of file
diff --git a/PL9fwy3NUQKwY9jl8xw-bWkVZkWcGGyspd/1ElgyngArXQ_raw.json b/PL9fwy3NUQKwY9jl8xw-bWkVZkWcGGyspd/1ElgyngArXQ_raw.json
new file mode 100644
index 0000000000000000000000000000000000000000..4b9eb5e2eb230e5af4a42e84c64d4dfcfe738359
--- /dev/null
+++ b/PL9fwy3NUQKwY9jl8xw-bWkVZkWcGGyspd/1ElgyngArXQ_raw.json
@@ -0,0 +1 @@
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"temperature": 1.0}, {"id": 31, "seek": 69121, "start": 670.27, "end": 691.21, "text": "the correct specimen identification أنا بدي أعرف أيش نوع العينة اللي بدي أستخدمها زي ما انتوا شفتوا لو مثلا لو بدي أسحب عينة دم مثلا بدي أعرف أنا في أي تيوب بدي أحطها المحاضرة الماضية احنا تحدثنا عن عدة أنواع من التيوبات كل تيوب فيها additive", "tokens": [3322, 3006, 34204, 22065, 41850, 4724, 16254, 5551, 3615, 28480, 36632, 8592, 8717, 45367, 18863, 9957, 3660, 13672, 1829, 4724, 16254, 5551, 14851, 9778, 40448, 11296, 30767, 1829, 19446, 16472, 2655, 14407, 13412, 5172, 2655, 14407, 45164, 50113, 15040, 45164, 4724, 16254, 5551, 3794, 5016, 3555, 6225, 9957, 3660, 11778, 2304, 50113, 15040, 4724, 16254, 5551, 3615, 28480, 41850, 8978, 36632, 6055, 1829, 37746, 4724, 16254, 5551, 5016, 9566, 11296, 9673, 5016, 46958, 25720, 9673, 46958, 10632, 1975, 5016, 8315, 6055, 24401, 12984, 8315, 18871, 6225, 41891, 14739, 14407, 3615, 9154, 16712, 1829, 37746, 9307, 28242, 6055, 1829, 37746, 8978, 11296, 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0.9143880208333334}, {"start": 1444.79, "end": 1445.23, "word": " أجتني", "probability": 0.92529296875}, {"start": 1445.23, "end": 1445.39, "word": " من", "probability": 0.99755859375}, {"start": 1445.39, "end": 1445.77, "word": " غرفة", "probability": 0.99267578125}, {"start": 1445.77, "end": 1446.21, "word": " الساحب", "probability": 0.83984375}, {"start": 1446.21, "end": 1446.39, "word": " على", "probability": 0.88037109375}, {"start": 1446.39, "end": 1447.53, "word": " المختبرات،", "probability": 0.91591796875}, {"start": 1447.53, "end": 1448.09, "word": " تمام؟", "probability": 0.9861653645833334}], "temperature": 1.0}, {"id": 66, "seek": 146781, "start": 1448.23, "end": 1467.81, "text": "فمعناها العينة لازم تكون إشمالها .. سيريلها labelled معلمة أو صار .. عملنا لها label كتبنا عليها معلومات مهمة لحتى ما يتم فقدانها أو ضياع عنها أو خربطتها أو يعني أنها يتم استبدالها بعينات أخرى", "tokens": [5172, 2304, 3615, 8315, 11296, 18863, 9957, 3660, 5296, 31377, 2304, 6055, 30544, 11933, 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"probability": 0.826220703125}, {"start": 1451.31, "end": 1451.43, "word": " ..", "probability": 0.42724609375}, {"start": 1451.43, "end": 1451.89, "word": " سيريلها", "probability": 0.8814697265625}, {"start": 1451.89, "end": 1452.63, "word": " labelled", "probability": 0.696044921875}, {"start": 1452.63, "end": 1454.03, "word": " معلمة", "probability": 0.7858072916666666}, {"start": 1454.03, "end": 1454.49, "word": " أو", "probability": 0.8671875}, {"start": 1454.49, "end": 1455.45, "word": " صار", "probability": 0.7274169921875}, {"start": 1455.45, "end": 1455.63, "word": " ..", "probability": 0.72314453125}, {"start": 1455.63, "end": 1456.15, "word": " عملنا", "probability": 0.9436848958333334}, {"start": 1456.15, "end": 1456.37, "word": " لها", "probability": 0.8251953125}, {"start": 1456.37, "end": 1456.77, "word": " label", "probability": 0.88818359375}, {"start": 1456.77, "end": 1457.35, "word": " كتبنا", "probability": 0.8619384765625}, {"start": 1457.35, "end": 1457.79, "word": " عليها", "probability": 0.99169921875}, {"start": 1457.79, "end": 1458.53, "word": " معلومات", "probability": 0.9886474609375}, {"start": 1458.53, "end": 1459.25, "word": " مهمة", "probability": 0.99365234375}, {"start": 1459.25, "end": 1460.05, "word": " لحتى", "probability": 0.89501953125}, {"start": 1460.05, "end": 1460.31, "word": " ما", "probability": 0.92822265625}, {"start": 1460.31, "end": 1460.81, "word": " يتم", "probability": 0.98193359375}, {"start": 1460.81, "end": 1461.51, "word": " فقدانها", "probability": 0.9967041015625}, {"start": 1461.51, "end": 1461.73, "word": " أو", "probability": 0.830078125}, {"start": 1461.73, "end": 1462.13, "word": " ضياع", "probability": 0.7489420572916666}, {"start": 1462.13, "end": 1462.55, "word": " عنها", "probability": 0.84130859375}, {"start": 1462.55, "end": 1462.79, "word": " أو", "probability": 0.9443359375}, {"start": 1462.79, "end": 1463.51, "word": " خربطتها", "probability": 0.98388671875}, {"start": 1463.51, "end": 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مهمة", "probability": 0.97705078125}, {"start": 1471.1, "end": 1471.44, "word": " جدا", "probability": 0.993408203125}, {"start": 1471.44, "end": 1471.62, "word": " يا", "probability": 0.9296875}, {"start": 1471.62, "end": 1472.04, "word": " بنات", "probability": 0.92431640625}, {"start": 1472.04, "end": 1472.58, "word": " فلازم", "probability": 0.883056640625}, {"start": 1472.58, "end": 1472.82, "word": " انا", "probability": 0.828369140625}, {"start": 1472.82, "end": 1473.26, "word": " اعمل", "probability": 0.9674479166666666}, {"start": 1473.26, "end": 1473.7, "word": " labeling", "probability": 0.8232421875}, {"start": 1473.7, "end": 1474.32, "word": " بشو", "probability": 0.8059895833333334}, {"start": 1474.32, "end": 1474.96, "word": " ب", "probability": 0.269775390625}, {"start": 1474.96, "end": 1475.26, "word": " ال", "probability": 0.4248046875}, {"start": 1475.26, "end": 1475.7, "word": " patients", "probability": 0.383544921875}, {"start": 1475.7, "end": 1476.24, "word": " surname", "probability": 0.84228515625}, {"start": 1476.24, "end": 1476.78, "word": " first", "probability": 0.89013671875}, {"start": 1476.78, "end": 1477.02, "word": " and", "probability": 0.96630859375}, {"start": 1477.02, "end": 1477.42, "word": " middle", "probability": 0.97265625}, {"start": 1477.42, "end": 1477.84, "word": " يعني", "probability": 0.86376953125}, {"start": 1477.84, "end": 1478.14, "word": " نفس", "probability": 0.988525390625}, {"start": 1478.14, "end": 1478.72, "word": " الاسم", "probability": 0.9064127604166666}, {"start": 1478.72, "end": 1479.54, "word": " بنفس", "probability": 0.87841796875}, {"start": 1479.54, "end": 1480.12, "word": " الطريقة", "probability": 0.83349609375}, {"start": 1480.12, "end": 1480.72, "word": " الكتابة", "probability": 0.992919921875}, {"start": 1480.72, "end": 1480.86, "word": " اللي", "probability": 0.760986328125}, {"start": 1480.86, "end": 1481.3, "word": " موجودة", "probability": 0.974609375}, {"start": 1481.3, "end": 1481.42, 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بعملية preparation of the puncture site كيف انا اسحب او احضر مكان السحب سواء كان vein puncture او skin puncture بدي اعقم المكان باستخدام 70% isopropanol طبعا 70% isopropanol يا بنات او تمام مش شرط يعني 70% isopropanol", "tokens": [2407, 39182, 39896, 4117, 4724, 44510, 10721, 4724, 25957, 1211, 10632, 13081, 295, 264, 27006, 540, 3621, 9122, 33911, 1975, 8315, 24525, 5016, 3555, 1975, 2407, 1975, 5016, 11242, 2288, 3714, 41361, 21136, 5016, 3555, 8608, 2407, 16606, 25961, 30669, 27006, 540, 1975, 2407, 3178, 27006, 540, 4724, 16254, 1975, 3615, 4587, 2304, 9673, 41361, 4724, 995, 14851, 9778, 3215, 10943, 5285, 4, 307, 404, 1513, 282, 401, 23032, 3555, 3615, 995, 5285, 4, 307, 404, 1513, 282, 401, 35186, 4724, 8315, 2655, 1975, 2407, 46811, 10943, 37893, 13412, 2288, 9566, 37495, 22653, 5285, 4, 307, 404, 1513, 282, 401], "avg_logprob": -0.10194925978632256, "compression_ratio": 1.6127450980392157, "no_speech_prob": 0.0, "words": [{"start": 2210.98, "end": 2211.22, "word": "و", 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"end": 4551.5, "word": " المريض", "probability": 0.9822591145833334}, {"start": 4551.5, "end": 4551.82, "word": " مرة", "probability": 0.717529296875}, {"start": 4551.82, "end": 4552.16, "word": " تانية", "probability": 0.9884440104166666}, {"start": 4552.16, "end": 4552.32, "word": " لو", "probability": 0.572265625}, {"start": 4552.32, "end": 4552.6, "word": " طلعت", "probability": 0.9773763020833334}, {"start": 4552.6, "end": 4553.08, "word": " superficial", "probability": 0.7958984375}, {"start": 4553.08, "end": 4553.42, "word": " بدي", "probability": 0.70361328125}, {"start": 4553.42, "end": 4553.7, "word": " ارجع", "probability": 0.8849283854166666}, {"start": 4553.7, "end": 4554.08, "word": " انخذه", "probability": 0.862548828125}, {"start": 4554.08, "end": 4554.28, "word": " مرة", "probability": 0.955810546875}, {"start": 4554.28, "end": 4554.52, "word": " تانية", "probability": 0.99560546875}, {"start": 4554.52, "end": 4554.88, "word": " وهذا", "probability": 0.822509765625}, 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{"start": 4571.05, "end": 4571.67, "word": " المختبر", "probability": 0.813720703125}, {"start": 4571.67, "end": 4572.17, "word": " يعني", "probability": 0.87646484375}, {"start": 4572.17, "end": 4572.49, "word": " مثلا", "probability": 0.93994140625}, {"start": 4572.49, "end": 4572.81, "word": " ممكن", "probability": 0.964111328125}, {"start": 4572.81, "end": 4573.25, "word": " يرفع", "probability": 0.9938151041666666}, {"start": 4573.25, "end": 4573.39, "word": " ال", "probability": 0.9794921875}, {"start": 4573.39, "end": 4573.59, "word": " white", "probability": 0.83251953125}, {"start": 4573.59, "end": 4574.05, "word": " pieces", "probability": 0.09490966796875}, {"start": 4574.05, "end": 4574.57, "word": " نتيجة", "probability": 0.973388671875}, {"start": 4574.57, "end": 4574.71, "word": " ال", "probability": 0.931640625}, {"start": 4574.71, "end": 4575.17, "word": " stress", "probability": 0.978515625}, {"start": 4575.17, "end": 4575.71, "word": " يعني", "probability": 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460647, "start": 4586.62, "end": 4606.48, "text": "فبالتالي أنا الأفضل أنه هذا المريض أستنى عليه نص ساعة على ساعة بعد ما يخلص يعني موجة البكاء اللي هو فيها أحاول أهدي أستنى عليه من نص ساعة على ساعة و بعدين أسحب له العينة طبعا ال thumb وال .. وال .. ال .. يعني الإصبع الكبير", "tokens": [5172, 3555, 6027, 2655, 6027, 1829, 41850, 16247, 5172, 11242, 1211, 14739, 3224, 23758, 9673, 16572, 11242, 5551, 14851, 1863, 7578, 47356, 8717, 9381, 8608, 995, 27884, 15844, 8608, 995, 27884, 39182, 19446, 7251, 9778, 1211, 9381, 37495, 22653, 3714, 29245, 3660, 29739, 4117, 16606, 13672, 1829, 31439, 8978, 11296, 5551, 5016, 995, 12610, 5551, 3224, 16254, 5551, 14851, 1863, 7578, 47356, 9154, 8717, 9381, 8608, 995, 27884, 15844, 8608, 995, 27884, 4032, 39182, 9957, 5551, 3794, 5016, 3555, 46740, 18863, 9957, 3660, 23032, 3555, 3615, 995, 2423, 258, 449, 65, 16070, 4386, 16070, 4386, 2423, 4386, 37495, 22653, 33688, 9381, 3555, 3615, 33251, 3555, 13546], "avg_logprob": -0.16530373386133498, 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"end": 4923.17, "word": " ما", "probability": 0.9306640625}, {"start": 4923.17, "end": 4923.47, "word": " بعد", "probability": 0.9580078125}, {"start": 4923.47, "end": 4923.91, "word": " السحب", "probability": 0.994140625}, {"start": 4923.91, "end": 4924.47, "word": " هي", "probability": 0.82568359375}, {"start": 4924.47, "end": 4924.75, "word": " اللي", "probability": 0.95947265625}, {"start": 4924.75, "end": 4925.35, "word": " مهمة", "probability": 0.9952799479166666}, {"start": 4925.35, "end": 4925.79, "word": " عشان", "probability": 0.9869791666666666}, {"start": 4925.79, "end": 4925.99, "word": " أنا", "probability": 0.48193359375}, {"start": 4925.99, "end": 4926.39, "word": " أحصل", "probability": 0.9689127604166666}, {"start": 4926.39, "end": 4926.69, "word": " على", "probability": 0.91943359375}, {"start": 4926.69, "end": 4927.03, "word": " ال", "probability": 0.98583984375}, {"start": 4927.03, "end": 4927.49, "word": " proper", "probability": 0.1475830078125}, {"start": 4927.49, "end": 4928.19, "word": " sample،", "probability": 0.73876953125}, {"start": 4928.19, "end": 4928.91, "word": " العينة", "probability": 0.8924153645833334}, {"start": 4928.91, "end": 4930.53, "word": " المثلة،", "probability": 0.74072265625}, {"start": 4930.53, "end": 4932.35, "word": " تمام؟", "probability": 0.92138671875}, {"start": 4932.35, "end": 4933.03, "word": " طيب", "probability": 0.9851888020833334}], "temperature": 1.0}, {"id": 221, "seek": 495537, "start": 4933.13, "end": 4955.37, "text": "رح أتوقف لهنا بالتسجيل لعند ال van puncture و ان شاء الله رح أسجل ال van puncture بتسجيل جديد يعني سحب العينة من وريد علشان يكون الحجم التسجيل مناسب و أيضا يعني بإمكانكم انكم انتوا تتعاملوا معاه يعني انا مايكونش في عندنا مشاكل في رفعه على ال drive او على يوتيوب او whatever", "tokens": [2288, 5016, 5551, 2655, 30543, 5172, 5296, 3224, 8315, 20666, 2655, 3794, 7435, 26895, 5296, 3615, 41260, 2423, 3161, 27006, 540, 4032, 16472, 13412, 16606, 21984, 12602, 5016, 5551, 3794, 7435, 1211, 2423, 3161, 27006, 540, 39894, 3794, 7435, 26895, 10874, 16254, 3215, 37495, 22653, 8608, 5016, 3555, 18863, 9957, 3660, 9154, 4032, 16572, 3215, 11203, 8592, 7649, 7251, 30544, 21542, 7435, 2304, 16712, 3794, 7435, 26895, 3714, 8315, 35457, 4032, 36632, 11242, 995, 37495, 22653, 4724, 28814, 2304, 41361, 24793, 16472, 24793, 16472, 2655, 14407, 6055, 2655, 3615, 10943, 1211, 14407, 20449, 40294, 37495, 22653, 1975, 8315, 19446, 1829, 30544, 8592, 8978, 43242, 8315, 37893, 995, 28820, 8978, 12602, 5172, 3615, 3224, 15844, 2423, 3332, 1975, 2407, 15844, 7251, 2407, 31371, 37746, 1975, 2407, 2035], "avg_logprob": -0.11552657761911708, "compression_ratio": 1.8582995951417005, "no_speech_prob": 6.556510925292969e-07, "words": [{"start": 4933.13, "end": 4933.39, "word": "رح", "probability": 0.758056640625}, {"start": 4933.39, "end": 4933.97, "word": " أتوقف", "probability": 0.9339599609375}, {"start": 4933.97, "end": 4934.31, "word": " لهنا", "probability": 0.7223307291666666}, {"start": 4934.31, "end": 4935.21, "word": " بالتسجيل", "probability": 0.9921875}, {"start": 4935.21, "end": 4935.83, "word": " لعند", "probability": 0.90380859375}, {"start": 4935.83, "end": 4935.95, "word": " ال", "probability": 0.98779296875}, {"start": 4935.95, "end": 4936.17, "word": " van", "probability": 0.791015625}, {"start": 4936.17, "end": 4936.69, "word": " puncture", "probability": 0.859619140625}, {"start": 4936.69, "end": 4936.83, "word": " و", "probability": 0.9794921875}, {"start": 4936.83, "end": 4936.91, "word": " ان", "probability": 0.650390625}, {"start": 4936.91, "end": 4937.11, "word": " شاء", "probability": 0.98828125}, {"start": 4937.11, "end": 4937.25, "word": " الله", "probability": 0.9599609375}, {"start": 4937.25, "end": 4937.49, "word": " رح", "probability": 0.9853515625}, {"start": 4937.49, "end": 4938.01, "word": " أسجل", "probability": 0.9180908203125}, {"start": 4938.01, "end": 4938.11, "word": " ال", "probability": 0.98779296875}, {"start": 4938.11, "end": 4938.33, "word": " van", "probability": 0.99169921875}, {"start": 4938.33, "end": 4938.89, "word": " puncture", "probability": 0.98876953125}, {"start": 4938.89, "end": 4940.53, "word": " بتسجيل", "probability": 0.922119140625}, {"start": 4940.53, "end": 4940.93, "word": " جديد", "probability": 0.9912109375}, {"start": 4940.93, "end": 4941.19, "word": " يعني", "probability": 0.795166015625}, {"start": 4941.19, "end": 4941.71, "word": " سحب", "probability": 0.8636067708333334}, {"start": 4941.71, "end": 4942.19, "word": " العينة", "probability": 0.8846028645833334}, {"start": 4942.19, "end": 4942.33, "word": " من", "probability": 0.99609375}, {"start": 4942.33, "end": 4942.85, "word": " وريد", "probability": 0.8746744791666666}, {"start": 4942.85, "end": 4943.85, "word": " علشان", "probability": 0.9724934895833334}, {"start": 4943.85, "end": 4944.31, "word": " يكون", "probability": 0.992431640625}, {"start": 4944.31, "end": 4944.95, "word": " الحجم", "probability": 0.99072265625}, {"start": 4944.95, "end": 4946.49, "word": " التسجيل", "probability": 0.9779052734375}, {"start": 4946.49, "end": 4947.23, "word": " مناسب", "probability": 0.9677734375}, {"start": 4947.23, "end": 4947.93, "word": " و", "probability": 0.92822265625}, {"start": 4947.93, "end": 4948.63, "word": " أيضا", "probability": 0.9381510416666666}, {"start": 4948.63, "end": 4948.85, "word": " يعني", "probability": 0.916259765625}, {"start": 4948.85, "end": 4949.45, "word": " بإمكانكم", "probability": 0.953515625}, {"start": 4949.45, "end": 4949.77, "word": " انكم", "probability": 0.5948486328125}, {"start": 4949.77, "end": 4950.01, "word": " انتوا", "probability": 0.7239583333333334}, {"start": 4950.01, "end": 4950.61, "word": " تتعاملوا", "probability": 0.986572265625}, {"start": 4950.61, "end": 4951.01, "word": " معاه", "probability": 0.988037109375}, {"start": 4951.01, "end": 4951.23, "word": " يعني", "probability": 0.956298828125}, {"start": 4951.23, "end": 4951.55, "word": " انا", "probability": 0.86328125}, {"start": 4951.55, "end": 4952.13, "word": " مايكونش", "probability": 0.8436279296875}, {"start": 4952.13, "end": 4952.25, "word": " في", "probability": 0.91943359375}, {"start": 4952.25, "end": 4952.47, "word": " عندنا", "probability": 0.7139892578125}, {"start": 4952.47, "end": 4952.87, "word": " مشاكل", "probability": 0.98779296875}, {"start": 4952.87, "end": 4953.01, "word": " في", "probability": 0.97314453125}, {"start": 4953.01, "end": 4953.37, "word": " رفعه", "probability": 0.9818115234375}, {"start": 4953.37, "end": 4953.55, "word": " على", "probability": 0.9248046875}, {"start": 4953.55, "end": 4953.67, "word": " ال", "probability": 0.5458984375}, {"start": 4953.67, "end": 4953.99, "word": " drive", "probability": 0.90625}, {"start": 4953.99, "end": 4954.23, "word": " او", "probability": 0.818359375}, {"start": 4954.23, "end": 4954.41, "word": " على", "probability": 0.94140625}, {"start": 4954.41, "end": 4954.89, "word": " يوتيوب", "probability": 0.791015625}, {"start": 4954.89, "end": 4955.11, "word": " او", "probability": 0.826416015625}, {"start": 4955.11, "end": 4955.37, "word": " whatever", "probability": 0.94775390625}], "temperature": 1.0}, {"id": 222, "seek": 496497, "start": 4955.76, "end": 4964.98, "text": "يعطيكم العافية، اقراكم ان شاء الله في ال part two of blood collection اللي بيتحدث عن ال van puncture", "tokens": [40228, 9566, 1829, 24793, 18863, 31845, 10632, 12399, 1975, 4587, 23557, 24793, 16472, 13412, 16606, 21984, 8978, 2423, 644, 732, 295, 3390, 5765, 13672, 1829, 4724, 36081, 24401, 12984, 18871, 2423, 3161, 27006, 540], "avg_logprob": -0.13939732228006635, "compression_ratio": 1.185483870967742, "no_speech_prob": 5.960464477539063e-08, "words": [{"start": 4955.76, "end": 4956.7, "word": "يعطيكم", "probability": 0.9091796875}, {"start": 4956.7, "end": 4957.56, "word": " العافية،", "probability": 0.8426513671875}, {"start": 4957.56, "end": 4957.94, "word": " اقراكم", "probability": 0.691650390625}, {"start": 4957.94, "end": 4958.1, "word": " ان", "probability": 0.7998046875}, {"start": 4958.1, "end": 4958.24, "word": " شاء", "probability": 0.99365234375}, {"start": 4958.24, "end": 4958.48, "word": " الله", "probability": 0.962890625}, {"start": 4958.48, "end": 4958.88, "word": " في", "probability": 0.9677734375}, {"start": 4958.88, "end": 4959.12, "word": " ال", "probability": 0.89013671875}, {"start": 4959.12, "end": 4959.5, "word": " part", "probability": 0.96728515625}, {"start": 4959.5, "end": 4959.98, "word": " two", "probability": 0.63232421875}, {"start": 4959.98, "end": 4960.66, "word": " of", "probability": 0.98681640625}, {"start": 4960.66, "end": 4962.0, "word": " blood", "probability": 0.90380859375}, {"start": 4962.0, "end": 4962.68, "word": " collection", "probability": 0.875}, {"start": 4962.68, "end": 4963.16, "word": " اللي", "probability": 0.9091796875}, {"start": 4963.16, "end": 4963.76, "word": " بيتحدث", "probability": 0.9666748046875}, {"start": 4963.76, "end": 4963.98, "word": " عن", "probability": 0.998046875}, {"start": 4963.98, "end": 4964.22, "word": " ال", "probability": 0.96484375}, {"start": 4964.22, "end": 4964.46, "word": " van", "probability": 0.80029296875}, {"start": 4964.46, "end": 4964.98, "word": " puncture", "probability": 0.96044921875}], "temperature": 1.0}], "language": "ar", "language_probability": 1.0, "duration": 4970.835, "duration_after_vad": 4899.939375000009}
\ No newline at end of file
diff --git a/PL9fwy3NUQKwY9jl8xw-bWkVZkWcGGyspd/1ElgyngArXQ_raw.srt b/PL9fwy3NUQKwY9jl8xw-bWkVZkWcGGyspd/1ElgyngArXQ_raw.srt
new file mode 100644
index 0000000000000000000000000000000000000000..7aae2c5eff8f8369436a5c05813d486300120690
--- /dev/null
+++ b/PL9fwy3NUQKwY9jl8xw-bWkVZkWcGGyspd/1ElgyngArXQ_raw.srt
@@ -0,0 +1,4344 @@
+1
+00:00:02,190 --> 00:00:06,830
+السلام عليكم ورحمة الله وبركاته طالبات العزيزات
+
+2
+00:00:06,830 --> 00:00:10,610
+المحاضرة الماضية كانت بعنوان blood component أي
+
+3
+00:00:10,610 --> 00:00:17,250
+يعني مكونات الدم حكينا عن مكونات الدم و أجزاءه و
+
+4
+00:00:17,250 --> 00:00:21,410
+حكينا عن خلايا الدم الفارث ما بين ال plasma و ال
+
+5
+00:00:21,410 --> 00:00:25,650
+serum ال anticoagulants مضادات التخطر اللي .. اللي
+
+6
+00:00:25,650 --> 00:00:30,010
+تم استخدامها يعني most common فيمختبرات ال
+
+7
+00:00:30,010 --> 00:00:34,750
+hematology و ان شاء الله اليوم محاضرتنا بعنوان
+
+8
+00:00:34,750 --> 00:00:38,650
+blood collection كيف انا اسحب عينة الدم او احصل
+
+9
+00:00:38,650 --> 00:00:43,670
+على عينة الدم من المريض blood collection المحاضرة
+
+10
+00:00:43,670 --> 00:00:48,710
+طويلة نوعا ما يعني انا دي 49 slide فلذلك ممكن انا
+
+11
+00:00:48,710 --> 00:00:54,170
+اجزقها ال part one and part two طيب طب خلينا نشوف
+
+12
+00:00:54,170 --> 00:01:00,120
+اش ما يتعلق بال blood collectionفي البداية راح
+
+13
+00:01:00,120 --> 00:01:04,940
+نحكي عن contact with patient التواصل مع المريض the
+
+14
+00:01:04,940 --> 00:01:09,980
+patient in hospital is anxious, fearful, ill
+
+15
+00:01:09,980 --> 00:01:12,860
+health and he is physically uncomfortable as a
+
+16
+00:01:12,860 --> 00:01:16,220
+result of his disease or injury he is also
+
+17
+00:01:16,220 --> 00:01:19,900
+separated from his family هلأ ال patient عادة
+
+18
+00:01:19,900 --> 00:01:24,500
+بيكون متوتر, anxious, fearful, خايف, ill health،
+
+19
+00:01:24,500 --> 00:01:30,240
+صحته مش مساعدة، مريضو أيضا هو غير مرتاح نتيجة مرضه
+
+20
+00:01:30,240 --> 00:01:37,100
+أو نتيجة يعني الإصابة أو الشكوى الصحية اللي هو عم
+
+21
+00:01:37,100 --> 00:01:41,720
+بيشك منها هلأ طبيعي أنه يكون كمان حتى مش بس عنده
+
+22
+00:01:41,720 --> 00:01:46,640
+تعب physical يعني تعب واضح ظاهر على جسدهممكن يكون
+
+23
+00:01:46,640 --> 00:01:51,200
+حتى كمان نفسيته تعبانة لإنه عنده قلق عنده توتر بما
+
+24
+00:01:51,200 --> 00:01:57,040
+يتعلق بصحته هل هذا العلة او المرض اللي هو مصابتي
+
+25
+00:01:57,040 --> 00:02:01,320
+هل هيودي بحياته او لأ he .. he is also separated
+
+26
+00:02:01,320 --> 00:02:06,700
+from his family هو ايضا اذا كان مريض مقيم في ال ..
+
+27
+00:02:06,880 --> 00:02:11,300
+المستشفى يعني موجود على أحد أسرة أقسام المستشفى
+
+28
+00:02:11,300 --> 00:02:16,940
+فبالتالي هو بيكون بعيد عن أسرته فهذا أيضا بيأثر
+
+29
+00:02:16,940 --> 00:02:20,600
+على نفسيته فبالتالي ال medical technologist must
+
+30
+00:02:20,600 --> 00:02:24,600
+show the patient at all time the kindness and
+
+31
+00:02:24,600 --> 00:02:28,020
+understanding that can mean some .. mean so much
+
+32
+00:02:28,190 --> 00:02:33,190
+هلأ يعني ال .. الشخص احنا كأخصائي تحاليل طبية انا
+
+33
+00:02:33,190 --> 00:02:39,430
+لازم اظهر تعاطفي و اظهر .. اظهر لطفي و ايضا تفهمي
+
+34
+00:02:39,430 --> 00:02:43,770
+لوضع المريض اللي هذا يعني راح يكون بيعني له الكثير
+
+35
+00:02:44,830 --> 00:02:49,430
+طيب بالنسبة لو كان المريض هذا عبارة عن a child
+
+36
+00:02:49,430 --> 00:02:53,750
+ممكن تكون تجربة تسحب عينة من a child عبارة عن
+
+37
+00:02:53,750 --> 00:02:58,150
+terrible experience بالنسبة لإله يعني تجربة سيئة
+
+38
+00:02:58,150 --> 00:03:04,050
+أو تجربة مخيفة جدا مريعة لإله و ممكن تكون
+
+39
+00:03:04,050 --> 00:03:06,990
+remembered and feared for that child for many
+
+40
+00:03:06,990 --> 00:03:11,230
+years يعني انها تظلها عالقة بذاكرته و تخوفه لسنوات
+
+41
+00:03:11,230 --> 00:03:17,490
+عديدةليش؟ لأنه ممكن تكون هذه التجربة، تجربة صح
+
+42
+00:03:17,490 --> 00:03:21,730
+بالعينة أو الحصول على العينة كانت سيئة أو مؤلمة أو
+
+43
+00:03:21,730 --> 00:03:25,190
+ما كانت بالاحتواء اللي يجب أو ينبغي أن تكون عليه
+
+44
+00:03:25,190 --> 00:03:29,460
+gain the child trust before the proceedingwith
+
+45
+00:03:29,460 --> 00:03:35,800
+blood collection لازم انه نكسب او نكسب ثقة الطفل
+
+46
+00:03:35,800 --> 00:03:40,260
+قبل لنبدأ بعملية سحب الدم the child should be
+
+47
+00:03:40,260 --> 00:03:43,720
+informed of what is going to happen if the child
+
+48
+00:03:43,720 --> 00:03:47,060
+is told that the puncture will not hurt the child
+
+49
+00:03:47,060 --> 00:03:49,760
+trust will be lost because this statement is
+
+50
+00:03:49,760 --> 00:03:54,360
+generally is not true هلأ احنا طبعا لازم نخبر
+
+51
+00:03:54,360 --> 00:04:02,340
+الطفل انهإيش إحنا راح نسوي يعني مثلا بالتعامل مع
+
+52
+00:04:02,340 --> 00:04:07,960
+الطفل يعني بيكون kindly أنا راح أفوت الإبرة و أسحب
+
+53
+00:04:07,960 --> 00:04:14,380
+منك عينة دم طبعا ما لازم أحكيله انه هذه الاشي مش
+
+54
+00:04:14,380 --> 00:04:18,220
+مؤلم لإن هو فعليا راح يتألم وبتالي هيك أنا راح
+
+55
+00:04:18,220 --> 00:04:22,100
+أخسر ثقته و راح أكون عنده terrible experience
+
+56
+00:04:22,100 --> 00:04:26,540
+يتذكرها و يخاف منها لسنوات تمام؟فبالتالي لازم انا
+
+57
+00:04:26,540 --> 00:04:29,020
+مثلا أحكيله بالتفصيل انا إيش بدي أعمل يعني أنا بدي
+
+58
+00:04:29,020 --> 00:04:31,620
+أسحب .. بدي .. بدي أنخزك شويه صغيرة و بدي أسحب منك
+
+59
+00:04:31,620 --> 00:04:36,160
+عينة دم عشان نعملك الفحص و نتطمن عليك بس أنت قوي
+
+60
+00:04:36,160 --> 00:04:40,040
+مش راح تتألم كتير هي التوجع شويه صغيرة أنخزها
+
+61
+00:04:40,040 --> 00:04:45,040
+صغيرة و أنت يعني كتير قوي و أنت راح تتحمل و أنت
+
+62
+00:04:45,040 --> 00:04:50,580
+أسد و .. من هذا الكلام رفعا لمعنوياته و نحاول أنه
+
+63
+00:04:50,580 --> 00:04:55,140
+احنا نكسب ثقتهطبعا بالنسبة لأ ال pheloptomy ال
+
+64
+00:04:55,140 --> 00:04:58,120
+procedure خلينا نشوف إيش معناة كلمة pheloptomy
+
+65
+00:04:58,120 --> 00:05:03,360
+phelop .. phelop لحالها معناها artery او معناها
+
+66
+00:05:03,360 --> 00:05:07,680
+vein .. vein sorry معناها vein وريد تمام و to me
+
+67
+00:05:07,680 --> 00:05:13,020
+معناها يعني to remove أو to isolate تمام يعني أن
+
+68
+00:05:13,020 --> 00:05:21,150
+أنا أعزل أو أستقصل أو أزيل تماممن الوريد تمام جزء
+
+69
+00:05:21,150 --> 00:05:24,790
+منه ايش هو الجزء اللي انا بده اشيله من الوريد ال
+
+70
+00:05:24,790 --> 00:05:30,490
+blood تمام اذا في look to me يعني عملية سحب عينة
+
+71
+00:05:30,490 --> 00:05:36,520
+دم من الوريد هاي معناها تمامطيب و نفس المعنى أيضًا
+
+72
+00:05:36,520 --> 00:05:41,360
+vein puncture إن أنا أسحب عينة من الوريد أيضًا
+
+73
+00:05:41,360 --> 00:05:44,820
+معناها vein puncture خلّيني أنا أشوف إيش مكتوب هنا
+
+74
+00:05:44,820 --> 00:05:48,720
+blood specimens عينات الدم are commonly obtained
+
+75
+00:05:48,720 --> 00:05:53,060
+from patients vein puncture under some
+
+76
+00:05:53,060 --> 00:05:56,640
+circumstances a skin puncture will be used for
+
+77
+00:05:56,640 --> 00:06:00,920
+this purposeهلأ أحيانا في بعض الحالات ممكن أنا
+
+78
+00:06:00,920 --> 00:06:04,600
+أعمل skin puncture لحتى أحصل على عينة الدم يعني
+
+79
+00:06:04,600 --> 00:06:08,160
+عينة الدم غالبا commonly obtained commonly
+
+80
+00:06:08,160 --> 00:06:13,580
+obtained يعني غالبا تعزل من الوريد أو يعني يحصل
+
+81
+00:06:13,580 --> 00:06:18,710
+عليها من الوريد، نحصل عليها من الوريدتمام؟ ولكن في
+
+82
+00:06:18,710 --> 00:06:22,750
+بعض الحالات أنا ممكن أحصل على عينة الدم من إحداث
+
+83
+00:06:22,750 --> 00:06:27,350
+.. يعني نخز في الاسكان كلمة puncture أكيد مرت
+
+84
+00:06:27,350 --> 00:06:31,210
+معاكم أنه مثلا puncture عجل السيارة، puncture عجل
+
+85
+00:06:31,210 --> 00:06:35,570
+الدراجة، تمام؟ إيش يعني؟ يعني صار في ثقوب، تمام؟
+
+86
+00:06:35,570 --> 00:06:38,870
+فلما أنا أحكي vain puncture يعني إحداث ثقوب في
+
+87
+00:06:38,870 --> 00:06:43,700
+الوريد للخروج أو لحصول على عينة الدماحداث ثقب في
+
+88
+00:06:43,700 --> 00:06:47,900
+ال skin لخروج أو الحصول على عينة دم، اذا puncture
+
+89
+00:06:47,900 --> 00:06:51,820
+يعني ثقب، تمام؟ اذا van puncture يعني حصول على
+
+90
+00:06:51,820 --> 00:06:57,550
+عينة دم من الوريدتمام؟ و skin puncture حصولة على
+
+91
+00:06:57,550 --> 00:07:01,610
+عينة دم من ال skin تمام؟ أكيد يعني ممكن مرتوا
+
+92
+00:07:01,610 --> 00:07:06,170
+بتجربة أن تروحوا على العيادات خصوصا عيادات الرعاية
+
+93
+00:07:06,170 --> 00:07:10,090
+الأولية مثل عيادات الواكالة و أنه لما بيدهم يسحبوا
+
+94
+00:07:10,090 --> 00:07:15,910
+منكوا معينة ينخذوا الإصبع تمام؟ و من خلال أداة
+
+95
+00:07:15,910 --> 00:07:21,340
+شفرةخاصة للنخز و الحصول على عينة دم من خلال ال
+
+96
+00:07:21,340 --> 00:07:25,300
+skin من خلال يعني انه انا ما .. ما .. ما استعملت
+
+97
+00:07:25,300 --> 00:07:29,940
+ابره و مادخلت جوا بريد تمام؟ هاي معناه ال skin
+
+98
+00:07:29,940 --> 00:07:35,700
+punctureبغض النظر عن طريقة سحب العينة irrespective
+
+99
+00:07:35,700 --> 00:07:40,420
+of the method use
+
+100
+00:07:40,420 --> 00:07:44,820
+certain techniques are common to all polyptomy
+
+101
+00:07:44,820 --> 00:07:48,440
+procedures هلأ أنا لازم في عدة خطوات بدي أحضرها
+
+102
+00:07:48,440 --> 00:07:55,680
+بدي أمر فيها بدي أتأكد منها أو أسويهاقبل لأحصل على
+
+103
+00:07:55,680 --> 00:07:59,860
+عينة الدم هذه أول إشي بدي أعمل ا check the request
+
+104
+00:07:59,860 --> 00:08:04,540
+form بدي أتأكد من ال request form اللي جاي مع
+
+105
+00:08:04,540 --> 00:08:13,680
+العينة هلأ في طبعا المختبرات المستشفة بتكون عجارة
+
+106
+00:08:13,680 --> 00:08:17,860
+عن عدة أقسام قسم ال hematology قسم ال chemistry ال
+
+107
+00:08:17,860 --> 00:08:22,220
+microbiology ال routine القسم ال ..الـ serology
+
+108
+00:08:22,220 --> 00:08:26,640
+المناعة فبالتالي عدة أقسام وبيكون في عندي غرفة
+
+109
+00:08:26,640 --> 00:08:31,640
+منفصلة عنهم اللي هي غرفة سحب العينات بنسميها ال
+
+110
+00:08:31,640 --> 00:08:34,340
+sample collection room تمام؟ هي ممكن تكون أيضا
+
+111
+00:08:34,340 --> 00:08:38,200
+كمان يعني مش شرط تكون موجودة متصلة مع المختبر ممكن
+
+112
+00:08:38,200 --> 00:08:41,600
+تكون يعني المختبر يكون على الطابق التاني غرفة سحب
+
+113
+00:08:41,600 --> 00:08:44,940
+العينات تكون على الطابق الأول زي مثلا ما هو موجود
+
+114
+00:08:44,940 --> 00:08:47,200
+بمستشفى الأوروبي تمام؟
+
+115
+00:08:49,050 --> 00:08:54,830
+فغرفة سحب العينات هذه بيصلها ال request form إيش
+
+116
+00:08:54,830 --> 00:08:58,450
+يعني ال request form؟ يعني طلب سحب العينة أو طلب
+
+117
+00:08:58,450 --> 00:09:06,290
+الفحصتمام و بيكون طبعا .. بيكون كتبه الطبيب في ..
+
+118
+00:09:06,290 --> 00:09:12,610
+في form خاص نموذج خاص لعينات و الحصات بيشيك عليها
+
+119
+00:09:12,610 --> 00:09:16,130
+الدكتور مثلا انا بدي عين .. بدي تعملي CBC او urine
+
+120
+00:09:16,130 --> 00:09:19,710
+test او urine analysis او stool analysis حسب ايش
+
+121
+00:09:19,710 --> 00:09:24,290
+نوع الفحص اللي مطلوب فب .. هذا الطلب الفحص اللي
+
+122
+00:09:24,290 --> 00:09:30,350
+بيجي اللي بيسمي request form بيجي مع المريضفأنا ب
+
+123
+00:09:30,350 --> 00:09:34,110
+.. باطلع عليه فبكون عارفة بناء أنا على ما عرفتي
+
+124
+00:09:34,110 --> 00:09:38,450
+وعلمي اللي درسته بكون عارفة إيش نوع العينة اللي
+
+125
+00:09:38,450 --> 00:09:44,670
+يجب أن نحصل عليها من المريض فبالتالي بصحب العينة
+
+126
+00:09:44,670 --> 00:09:47,790
+إذا .. إذا بده عينة دم بصحب عينة دم إذا بده عينة
+
+127
+00:09:47,790 --> 00:09:51,510
+urine بخليه هو يجمع عينة urine و بعطي تعليمات حول
+
+128
+00:09:51,510 --> 00:09:54,770
+الجامعة عين تستول و يجمع عين تستول و بعطي تعليمات
+
+129
+00:09:54,770 --> 00:09:58,730
+حول الجامعةفيعني احنا ذكرنا قبل هيك انه في عينات
+
+130
+00:09:58,730 --> 00:10:01,430
+انا بجمعها بنفسي، في عينات انا بعطي تعليمات للمريض
+
+131
+00:10:01,430 --> 00:10:05,810
+كيف يجمعها و فيها تعليمات انا بعطي معلومات للمريض
+
+132
+00:10:05,810 --> 00:10:09,370
+انه كيف يحضر حاله للفحص مثلا يصوم او ياكل اشي معين
+
+133
+00:10:09,370 --> 00:10:12,350
+او يفطر على اشي معين او يمتنع عن اكل اشياء معينة
+
+134
+00:10:12,350 --> 00:10:16,460
+حسبتمام؟ فبالتالي ال request form إذا هو الطلب
+
+135
+00:10:16,460 --> 00:10:20,280
+اللي بيكتبه المريض أو بيكون هو نموذج، معادل بس على
+
+136
+00:10:20,280 --> 00:10:24,940
+الدكتور يعمل test، يحط صح جنب الفحص اللي هو بدويا
+
+137
+00:10:24,940 --> 00:10:30,390
+نجري للمريض، تمام؟الخطوة تانية اذا انا بدي اشيك
+
+138
+00:10:30,390 --> 00:10:33,630
+على هذا ال request form بدي اتأكد ان هو معبق بشكل
+
+139
+00:10:33,630 --> 00:10:37,130
+صحيح او لأ من قبل الطبيب the correct patient
+
+140
+00:10:37,130 --> 00:10:42,290
+identification بدي ا .. اتعرف على المريض بشكل صحيح
+
+141
+00:10:42,290 --> 00:10:45,570
+اتأكد انه هذا هو المريض اللي انا بدي اجمع منه
+
+142
+00:10:45,570 --> 00:10:52,950
+العين او لأيعني ماروحش أجمع عينة من مريض آخر ويكون
+
+143
+00:10:52,950 --> 00:10:57,450
+يعني مثلا هيك بكون أنا عاملك mix ما بين النموذج
+
+144
+00:10:57,450 --> 00:11:00,570
+يعني يكون النموذج من مريض و العينة من مريض آخر
+
+145
+00:11:00,570 --> 00:11:05,010
+فبالتالي النتائج بتكونش معبرة تمام؟ بيصير في خلط و
+
+146
+00:11:05,010 --> 00:11:09,590
+خربطة في ما بين ال requestات form و ما بين العينات
+
+147
+00:11:10,270 --> 00:11:13,730
+the correct specimen identification أنا بدي أعرف
+
+148
+00:11:13,730 --> 00:11:18,550
+أيش نوع العينة اللي بدي أستخدمها زي ما انتوا شفتوا
+
+149
+00:11:18,550 --> 00:11:23,090
+لو مثلا لو بدي أسحب عينة دم مثلا بدي أعرف أنا في
+
+150
+00:11:23,090 --> 00:11:27,330
+أي تيوب بدي أحطها المحاضرة الماضية احنا تحدثنا عن
+
+151
+00:11:27,330 --> 00:11:32,020
+عدة أنواع من التيوبات كل تيوب فيها additiveفيها
+
+152
+00:11:32,020 --> 00:11:37,340
+إضافة فيها anticoagulant مختلف عن الأخرى فبالتاني
+
+153
+00:11:37,340 --> 00:11:40,840
+أنا بده اميز فيه فحوصات بتنعمل على heparin فيه
+
+154
+00:11:40,840 --> 00:11:44,900
+فحوصات بتنعمل على citrate شرط لازم يكون citrate في
+
+155
+00:11:44,900 --> 00:11:48,880
+العينة فيه فحوصات على edita فحسب إيش نوع ال
+
+156
+00:11:48,880 --> 00:11:52,400
+anticoagulant الموجود أو ال additive الموجود فيه
+
+157
+00:11:52,400 --> 00:11:56,300
+فحوصات لأ لازم العينة تتجلط و أنا أفصل serum فلازم
+
+158
+00:11:56,300 --> 00:11:59,340
+أحطها في planet tube أو التيوبات اللي فيها clot
+
+159
+00:11:59,340 --> 00:12:03,680
+activatorفهذه كلها طبعا انا بدي اميزها بدي اعرف
+
+160
+00:12:03,680 --> 00:12:08,460
+انا ايش نوع العينة المطلوب عشان احصل على ايش
+
+161
+00:12:08,460 --> 00:12:11,600
+correct specimen identification انا عرفت على نوع
+
+162
+00:12:11,600 --> 00:12:18,710
+العينة الصحيح اللي انا بدي احصل عليه تمام؟فلازم
+
+163
+00:12:18,710 --> 00:12:24,150
+أيضا أعمل إجراءات تضمن ال safety تضمن الأمان
+
+164
+00:12:24,150 --> 00:12:28,710
+والسلامة و ال infection control و الحد من انتقال
+
+165
+00:12:28,710 --> 00:12:32,350
+العدوى لمين؟ أنا حكيت بدي أحافظ .. أحافظ على
+
+166
+00:12:32,350 --> 00:12:37,010
+سلامتي أولا و سلامة ال patientو سلامة ال
+
+167
+00:12:37,010 --> 00:12:40,650
+colleagues, الزملاء اللي بيشتغلوا بالمختبر، تمام؟
+
+168
+00:12:40,650 --> 00:12:46,690
+و أيضا بدي بعد هيك أبدأ أحضر مكان ال puncture
+
+169
+00:12:46,690 --> 00:12:50,530
+المكان اللي أنا بدي أسحب منه عينة دم سواء كان vein
+
+170
+00:12:50,530 --> 00:12:55,050
+أو سواء كان skin فبدي أحضره علشان أبدأ في سحب
+
+171
+00:12:55,050 --> 00:12:59,540
+العينةتمام؟ إذا هذه الخطوات بغض النظر عن عملية
+
+172
+00:12:59,540 --> 00:13:06,300
+السحب ذات نفسها أنا لازم أقوم فيها في كل مرة بسحب
+
+173
+00:13:06,300 --> 00:13:11,220
+فيها عينا من مريض، تمام؟ طيب، انشوف
+
+174
+00:13:13,800 --> 00:13:17,740
+إيش يعني .. نشوف هلأ ال slides الجاية راح تحكي عن
+
+175
+00:13:17,740 --> 00:13:22,720
+كل واحدة بالدور إيش هما؟ تمام؟ تفاصيل أكتر نحكي عن
+
+176
+00:13:22,720 --> 00:13:27,060
+ال request form هلأ ال request form هو عبارة عن
+
+177
+00:13:27,060 --> 00:13:30,700
+نموذج ممكن يكون اسمه request form أو requisition
+
+178
+00:13:30,700 --> 00:13:35,160
+form نفس المعنى طلب، نموذج طلب، لفحص، نموذج طلب
+
+179
+00:13:35,160 --> 00:13:41,310
+إجراء فحص، هك اسمهطبعا لازم كل عينة تأتي للمختبر
+
+180
+00:13:41,310 --> 00:13:45,310
+من غرفة جمع العينات لازم يكون معها ال request
+
+181
+00:13:45,310 --> 00:13:49,490
+الخاص فيها، هلأ صارت ال requestات يا بنات تيجي
+
+182
+00:13:49,490 --> 00:13:52,890
+إلكتروني لإنه صار في عندي حاجة اسمها lab
+
+183
+00:13:52,890 --> 00:13:57,760
+informaticsبس زمان كان في ورقي بس المهم بغض النظر
+
+184
+00:13:57,760 --> 00:14:01,520
+كان النظام المعمول به بالمستشفى ورقي أو إلكتروني
+
+185
+00:14:01,520 --> 00:14:05,680
+لازم يكون كل عينة بتنشغل في المختبر يكون فيه ال
+
+186
+00:14:05,680 --> 00:14:08,920
+Hercules يعني مجرد ما أنا أستلم العينة إذا أنا
+
+187
+00:14:08,920 --> 00:14:12,160
+بشتغل إلكتروني بدي أروح أحدث البرنامج و أشوف هل
+
+188
+00:14:12,160 --> 00:14:16,630
+وصلني طلب لهذه العينة أو لاطبعا؟ طيب العينة ..
+
+189
+00:14:16,630 --> 00:14:21,190
+الطلب هذا the requisition form must contain the
+
+190
+00:14:21,190 --> 00:14:23,770
+proper information in order to process the
+
+191
+00:14:23,770 --> 00:14:28,430
+specimen لازم يكون موجود فيه المعلومات الكافية و
+
+192
+00:14:28,430 --> 00:14:32,750
+الجيدة لحت .. لحتى أنا أشتغل على العينة طبعا؟
+
+193
+00:14:32,750 --> 00:14:38,710
+معلومات دقيقة بتتعلق بالمريد و العينة و كل إشي ب
+
+194
+00:14:38,710 --> 00:14:43,590
+.. متعلق يعني في عملية السحب لحتى أنا أقدر أشتغل
+
+195
+00:14:43,590 --> 00:14:47,630
+الفحصتمام؟ و .. او احكم هل العينها دي صالحة لإجراء
+
+196
+00:14:47,630 --> 00:14:51,390
+الفحص او لأ The essential element of requisition
+
+197
+00:14:51,390 --> 00:14:57,390
+form are العناصر المكونة لطلب الفحص هو اول حاجة ال
+
+198
+00:14:57,390 --> 00:15:01,410
+patient surname, first name and middle initial
+
+199
+00:15:01,410 --> 00:15:09,170
+لازم يكون موجود فيالطلب اسم ال .. اسم المريض كاملا
+
+200
+00:15:09,170 --> 00:15:13,790
+ال surname اسم العائلة و ال first name اسم .. اسمه
+
+201
+00:15:13,790 --> 00:15:18,810
+الأولى يعني مثلا أنا نور أبوتايم تمام؟ لازم يكون
+
+202
+00:15:18,810 --> 00:15:22,510
+أبوتايم نور و اذا .. و ال middle initial يعني حرف
+
+203
+00:15:22,510 --> 00:15:27,780
+ال I من اسم الوالد إبراهيمتمام؟ هذا ال .. ال .. ال
+
+204
+00:15:27,780 --> 00:15:31,480
+band الأول معناه انه لازم يكون في عندي الاسم
+
+205
+00:15:31,480 --> 00:15:35,040
+العائلة و الاسم الأول و ال middle initial الحرف
+
+206
+00:15:35,040 --> 00:15:39,540
+الأول من اسم الوالد patient ID number طبعا احنا
+
+207
+00:15:39,540 --> 00:15:44,880
+نعرف بالمستشفى عن طريق numbers احنا عبارة عن رقم
+
+208
+00:15:44,880 --> 00:15:50,920
+.. رقم ملفي هو رقمي الطبيلازم هذا .. طبعا هذا اللي
+
+209
+00:15:50,920 --> 00:15:54,580
+أنا من خلاله بيتعرفوا عليا في المستشفى من خلال
+
+210
+00:15:54,580 --> 00:16:01,520
+طبعا الفحص بيجي مرفق برقمي الخاص الأدوية بتصرف
+
+211
+00:16:01,520 --> 00:16:05,760
+تبعا لرقمي الخاص صور الأشعة حسب رقمي التمريد
+
+212
+00:16:05,760 --> 00:16:09,500
+الفحوصات الفيزيائية العلاج الطبيعي كل شيء بيكون
+
+213
+00:16:09,500 --> 00:16:13,020
+طبعا بيتعرفوا عليا من خلال الرقمي الخاص فيا
+
+214
+00:16:14,040 --> 00:16:18,400
+patient's date of birth and sex لازم يكون في عندى
+
+215
+00:16:18,400 --> 00:16:23,100
+تاريخ الميلاد طبعا و لازم يكون ال gender معروف
+
+216
+00:16:23,100 --> 00:16:28,060
+male or female لازم يكون مضوّن بعدين ال
+
+217
+00:16:28,060 --> 00:16:33,540
+requisition physicianscomplete name تمام؟ و أيضا
+
+218
+00:16:33,540 --> 00:16:40,040
+اللي لازم يكون الاسم الكامل للطبيب اللي طلب هذا
+
+219
+00:16:40,040 --> 00:16:44,360
+الفحص أو طلب الصح بالعينة indicating the test
+
+220
+00:16:44,360 --> 00:16:48,560
+requested و أيضا لازم يكون الطبيب هذا معلم على
+
+221
+00:16:48,560 --> 00:16:52,780
+الفحوصات اللي احنا بدنا نشتغلها source of specimen
+
+222
+00:16:53,920 --> 00:16:57,580
+طبعا this information must be given when
+
+223
+00:16:57,580 --> 00:17:01,180
+requesting microbiology, cytology, fluid analysis
+
+224
+00:17:01,180 --> 00:17:05,480
+or other testing where analysis is reporting and
+
+225
+00:17:05,480 --> 00:17:11,880
+is site specific هلأ أحيانا بيكون الطبيبمعينلي ال
+
+226
+00:17:11,880 --> 00:17:15,460
+source of specimen يعني أسحبيلي عينة من المكان
+
+227
+00:17:15,460 --> 00:17:19,080
+الفلاني أنا بدي خصوصا إذا كان بديها لل
+
+228
+00:17:19,080 --> 00:17:22,880
+microbiology إذا أحنا بنعمل مزرعة أو إذا كان بدنا
+
+229
+00:17:22,880 --> 00:17:27,020
+ندرس الخلايا نشوف هل في خلايا سرطانية يعني عفوا أو
+
+230
+00:17:27,020 --> 00:17:35,720
+لأ بدي أشوف فحوصات ال سواق الجسم بده مثلا فحص من
+
+231
+00:17:35,720 --> 00:17:43,790
+جزء من الجلد ملتهبو .. الطبيب شاكت إنه في عدوى
+
+232
+00:17:43,790 --> 00:17:48,010
+فطرية .. فطريات على الجلد فأنا لازم من هذه المكان
+
+233
+00:17:48,010 --> 00:17:52,490
+أخد مسحة من ال .. ال .. فما .. فما بنفع يجي ..
+
+234
+00:17:52,490 --> 00:17:58,770
+يعني ما بنفع يسيب ليها يعني عايمة مش معروفةلازم
+
+235
+00:17:58,770 --> 00:18:03,150
+انا يحددلي انه من المكان الفلاني مثلا من الإيد
+
+236
+00:18:03,150 --> 00:18:10,650
+اليمنى فوق الرسغ مثلا موجود في منطقة infection
+
+237
+00:18:10,650 --> 00:18:13,710
+منها انا بدي سحب عينة عشان اشوف ايش نوع ال
+
+238
+00:18:13,710 --> 00:18:17,030
+infection مثلا فطريات ولا بكتيريا او whatever
+
+239
+00:18:17,030 --> 00:18:21,750
+فبالتالي لما يكون المكان ال site specific لازم انا
+
+240
+00:18:21,750 --> 00:18:27,080
+اسحب من مكان معين لازم هذاالطبيب يحددلي source of
+
+241
+00:18:27,080 --> 00:18:31,200
+specimen مصدر العينة date and time of collection
+
+242
+00:18:31,200 --> 00:18:38,920
+أيضا التاريخ ووقت سحب العينة لازم يكون يعني مكتوب
+
+243
+00:18:38,920 --> 00:18:44,370
+و ال initial of the optomist برضه كمان لازمالاسم
+
+244
+00:18:44,370 --> 00:18:48,730
+الأول من الشخص اللي سحب العينة يكون موجود أو مدكر
+
+245
+00:18:48,730 --> 00:18:53,610
+يعني في معلومات بعديها الطبيب يعني اسم المريض و ال
+
+246
+00:18:53,610 --> 00:18:57,390
+ID number ال date of birth ال gender ال
+
+247
+00:18:57,390 --> 00:19:00,450
+requisition physician complete name و ال
+
+248
+00:19:00,450 --> 00:19:02,990
+indicating test و ال source of specimen هدول
+
+249
+00:19:02,990 --> 00:19:07,850
+بعبيهم الطبيباحنا بعد ما أنا أاخد ال request من
+
+250
+00:19:07,850 --> 00:19:12,670
+المريض و أصحبله العينة لازم أسجل الوقت و التاريخ
+
+251
+00:19:12,670 --> 00:19:17,830
+لصحب العينة و أيضا الاسم الأول من اسمي، نور، تمام؟
+
+252
+00:19:17,830 --> 00:19:22,330
+فهكذا بكتب على ال request form، تمام؟ اذا جزء يعبق
+
+253
+00:19:22,330 --> 00:19:26,510
+من خلال أو يملق من خلال الطبيب و جزء يملق من خلال
+
+254
+00:19:26,510 --> 00:19:30,230
+ال .. ال philoptomist أو اللي هو أخصائي المختبرات
+
+255
+00:19:30,230 --> 00:19:35,610
+اللي بيجمع العينة، تمام؟طيب patient identification
+
+256
+00:19:35,610 --> 00:19:40,990
+أثناء وصول ال request تمام؟ و أنا بدي أسحب العينة
+
+257
+00:19:40,990 --> 00:19:44,470
+من المريض لازم أعمل حاجة اسمها patient
+
+258
+00:19:44,470 --> 00:19:49,210
+identification أنا بدي أميز أن هو مريض أو أي مريض
+
+259
+00:19:49,210 --> 00:19:53,090
+أنا بدي أسحب منه العينة تمام؟ف is it critical for
+
+260
+00:19:53,090 --> 00:19:56,570
+hospital patient that is accomplished by checking
+
+261
+00:19:56,570 --> 00:20:02,030
+the identification wristband؟ إذا الشخص مقيم على
+
+262
+00:20:02,030 --> 00:20:05,790
+.. في المستشفى أو يعني عنده بيات في المستشفى هو
+
+263
+00:20:05,790 --> 00:20:10,270
+hospital patient، تمام؟ أو أحيانا بسميها in
+
+264
+00:20:10,270 --> 00:20:15,270
+patient، بحط كلمة in، تمام؟بحط كلمة in هنا بيصير
+
+265
+00:20:15,270 --> 00:20:21,050
+inpatient طبعا إذا كان كالشخص .. المريض inpatient
+
+266
+00:20:21,050 --> 00:20:25,330
+يعني patient مريض داخل المستشفى أو hospital
+
+267
+00:20:25,330 --> 00:20:29,990
+patient طبعا إذا كان هذا الطبيب في داخل المستشفى
+
+268
+00:20:29,990 --> 00:20:33,810
+على أحد الأسرة فأنا أو إذا كان خصوصا فاقد الوعي
+
+269
+00:20:33,810 --> 00:20:39,030
+يعني أو مغيب بيكون في بإيده حاجة اسمها wristband
+
+270
+00:20:39,030 --> 00:20:44,680
+طبعايعني اللي على الرسخ على الكوع يعني لأ مش الكوع
+
+271
+00:20:44,680 --> 00:20:51,700
+الرسخ عند إيده هنا بيكون في اسورة هو لابسها و فيها
+
+272
+00:20:51,700 --> 00:20:58,780
+معلوماته الخاصة اسمه و رقمه و ال .. مثلا يعني أي
+
+273
+00:20:58,780 --> 00:21:02,260
+معلومات مهمة بتتعلق بالمريد النام و ال hospital
+
+274
+00:21:02,260 --> 00:21:05,820
+نفس ال .. الاسورة اللي بتكون في إيدي المولود أول
+
+275
+00:21:05,820 --> 00:21:10,470
+ما بينولد بن .. بنكتب اسم فلان اذن فلانةو ال .. ال
+
+276
+00:21:10,470 --> 00:21:15,830
+number تبعه و تاريخ الولادة و مثلا وزنه هدى
+
+277
+00:21:15,830 --> 00:21:18,690
+معلومات بتتعلق فى المريض او بتتعلق فى الباب اللى
+
+278
+00:21:18,690 --> 00:21:22,510
+انولد تمام؟ فأيضا نفس الاشي المريض اللى بيكون على
+
+279
+00:21:22,510 --> 00:21:25,170
+أسرة المستشفى بيكون في عنده .. المفروض يكون في
+
+280
+00:21:25,170 --> 00:21:30,200
+عنده wrist band يعنى قسورة فى إيدهو مكتوب فيها على
+
+281
+00:21:30,200 --> 00:21:33,480
+الأقل النام و ال hospital و بعض المعلومات المتعلقة
+
+282
+00:21:33,480 --> 00:21:37,400
+فيه فانا لما باجي بدي أسحب من عينة من مريض خصوصا
+
+283
+00:21:37,400 --> 00:21:41,040
+لو كان فاقد للواعي او مغيب بدي ا check عليها
+
+284
+00:21:41,040 --> 00:21:44,800
+الاسورة و أشوف المعلومات اللي هو اللي عليها مطابقة
+
+285
+00:21:44,800 --> 00:21:46,920
+لل request عشان أعرف ان هو هذا المريض اللي بدي
+
+286
+00:21:46,920 --> 00:21:51,720
+أسحب منه او لاطبعا إذا كان in patient صاحي بقوله
+
+287
+00:21:51,720 --> 00:21:55,520
+بدي أتكلم معه بدي أحكيله أنت فلان إيش إسمك؟ طب إيش
+
+288
+00:21:55,520 --> 00:22:01,980
+رقمك؟ طب إيش إسمك بالكامل؟ جدش عمرك؟ عم بدي أسأله
+
+289
+00:22:01,980 --> 00:22:05,280
+بعض الأسئلة أكرر بعض الأسئلة اللي موجودة في ال
+
+290
+00:22:05,280 --> 00:22:09,060
+request عشان أتأكد إن هو هذا الشخص اللي موجود ..
+
+291
+00:22:09,060 --> 00:22:12,120
+اللي ممنشن إله أو اللي مشارقله في ال request بدي
+
+292
+00:22:12,120 --> 00:22:17,930
+أسحب منه عينة أو لأ طبعا؟ طيبإذا كان المريض عبارة
+
+293
+00:22:17,930 --> 00:22:21,650
+عن outpatient، إيش يعني outpatient؟ يعني هو مريض
+
+294
+00:22:21,650 --> 00:22:27,810
+خارج المستشفى، إذا إجا .. إجا لحتى يزور المستشفى،
+
+295
+00:22:27,810 --> 00:22:32,560
+يعني إجا على العيادات الخارجية للمستشفىتمام؟ و بده
+
+296
+00:22:32,560 --> 00:22:38,040
+يتعالج و يطلبوله فحص مثلا معين فساعتها أنا بدي
+
+297
+00:22:38,040 --> 00:22:42,880
+أتعامل مع المريض هذا بدي أعمل .. بدي أسأله ask the
+
+298
+00:22:42,880 --> 00:22:47,260
+patient for his or her full name بدي أسأله عن اسمه
+
+299
+00:22:47,260 --> 00:22:51,940
+بالكامل عن بعض المعلومات المتعلقة to be verified
+
+300
+00:22:51,940 --> 00:22:59,590
+عشان أتأكدإنه هذا هو الشخص المعني بال request form
+
+301
+00:22:59,590 --> 00:23:04,070
+أو هو الشخص اللي .. اللي لازم أنا أسحب منه عينة و
+
+302
+00:23:04,070 --> 00:23:08,630
+هذا ال request إله أو لأ، تمام؟ إذا نوعي المرضى
+
+303
+00:23:08,630 --> 00:23:12,470
+نعين، إما بيكون inpatient أو outpatient، تمام؟ ال
+
+304
+00:23:12,470 --> 00:23:17,630
+inpatient ممكن يكون فاقد للوعي، نايم على الأسرة،
+
+305
+00:23:17,630 --> 00:23:21,370
+لا يمكن التواصل معاه، verbal communication، مش
+
+306
+00:23:21,370 --> 00:23:25,830
+عارفة أتكلم معاهفبدي أشيك على wristband إذا كان هو
+
+307
+00:23:25,830 --> 00:23:30,110
+صاحي فبسأله أما ال out patient طالما هو خارج
+
+308
+00:23:30,110 --> 00:23:32,410
+المستشفى و إيجي على العيادات الخارجية إذا ان هو
+
+309
+00:23:32,410 --> 00:23:39,150
+إيجي واعي معناها بدي أسأله اسمه بالكامل بعض
+
+310
+00:23:39,150 --> 00:23:42,870
+المعلومات اللي موجودة في ال request و عشان يأكدلي
+
+311
+00:23:42,870 --> 00:23:47,570
+أن هذا ال request له زي تاريخ ميلادهمكان سكنه، أي
+
+312
+00:23:47,570 --> 00:23:51,250
+إشي بتعلق فيه داخل ال request بسأله عنه عشان أشوف
+
+313
+00:23:51,250 --> 00:23:54,330
+إجابته صحيحة أو لأ، هو هذا الشخص اللي .. اللي طلب
+
+314
+00:23:54,330 --> 00:23:59,290
+إله أو لأ، تمام؟ طيب، هذا .. إذن هذا بالنسبة لل
+
+315
+00:23:59,290 --> 00:24:02,150
+patient identification بالنسبة لل specimen
+
+316
+00:24:02,150 --> 00:24:06,390
+identification، لو العينة أجتني من غرفة الساحب على
+
+317
+00:24:06,390 --> 00:24:11,310
+المختبرات، تمام؟فمعناها العينة لازم تكون إشمالها
+
+318
+00:24:11,310 --> 00:24:16,370
+.. سيريلها labelled معلمة أو صار .. عملنا لها
+
+319
+00:24:16,370 --> 00:24:21,510
+label كتبنا عليها معلومات مهمة لحتى ما يتم فقدانها
+
+320
+00:24:21,510 --> 00:24:26,710
+أو ضياع عنها أو خربطتها أو يعني أنها يتم استبدالها
+
+321
+00:24:26,710 --> 00:24:32,040
+بعينات أخرىعملية ال labeling مهمة جدا يا بنات
+
+322
+00:24:32,040 --> 00:24:36,240
+فلازم انا اعمل labeling بشو ب ال patients surname
+
+323
+00:24:36,240 --> 00:24:40,120
+first and middle يعني نفس الاسم بنفس الطريقة
+
+324
+00:24:40,120 --> 00:24:43,140
+الكتابة اللي موجودة في ال request بكتبها على
+
+325
+00:24:43,140 --> 00:24:50,880
+العينة ايضا ال ID number الرقم المعرف للمريض سواق
+
+326
+00:24:50,880 --> 00:24:56,390
+كان رقم هويته او رقم الطبأو رقم ملفه حسب يعني
+
+327
+00:24:56,390 --> 00:25:00,170
+المستشفى إيش نظامها في تعريف المرضى، في ناس في
+
+328
+00:25:00,170 --> 00:25:03,350
+مستشفيات بتعرف المرضى من خلال رقم هويتهم، في لأ
+
+329
+00:25:03,350 --> 00:25:08,810
+بتعطيهم رقم هي هذا رقم الطبي أو رقم ملفه، تمام؟
+
+330
+00:25:08,810 --> 00:25:11,970
+date, time and initial of the phileptomist لازم
+
+331
+00:25:11,970 --> 00:25:15,050
+أيضا الجزئية اللي بدي أعبيها أنا ع ال request اللي
+
+332
+00:25:15,050 --> 00:25:19,250
+هي تاريخ ووقت سحب العينة و ال initial تبعي اسم
+
+333
+00:25:19,250 --> 00:25:23,520
+الأول لازم أيضا أكتبها على العينةعلشان لو في أي
+
+334
+00:25:23,520 --> 00:25:27,920
+مشا .. مشاكل يرجعولي، تمام؟ أي خربطة أو أي مشاكل
+
+335
+00:25:27,920 --> 00:25:31,840
+يرجعولي مثلا ب .. بيتعلق بسحب العينة علشان أنا
+
+336
+00:25:31,840 --> 00:25:34,840
+أفيدهم طيب
+
+337
+00:25:37,770 --> 00:25:42,370
+Safety and infection control أيضا لازم .. لازم
+
+338
+00:25:42,370 --> 00:25:47,910
+أراعي ال .. ال .. الأمان والسلامة و أيضا أحب
+
+339
+00:25:47,910 --> 00:25:52,490
+انتقال العدوى في .. أثناء سحب العينة تمام؟ أثناء
+
+340
+00:25:52,490 --> 00:25:57,610
+الحصول على العينةفبالتالي لازم طبعا انا لما بدأ
+
+341
+00:25:57,610 --> 00:26:01,450
+اتواصل مع ال .. ال .. ال sick patient و عيناتهم
+
+342
+00:26:01,450 --> 00:26:05,450
+طبعا انا بسحب عينة دم يا بناة عينة دم يعني انا
+
+343
+00:26:05,450 --> 00:26:09,010
+معرضة لمثلا انه لا سمح الله، لا سمح الله، اذا
+
+344
+00:26:09,010 --> 00:26:14,550
+المريض مثلا مصاب ب hepatitis، ب virus ال AIDSبأي
+
+345
+00:26:14,550 --> 00:26:18,990
+فيروس خطير ممكن ينتقل لإلي عبر الدم، تمام؟ إذا لو
+
+346
+00:26:18,990 --> 00:26:23,150
+مثلا أنا ما اتبعت القواعد السلامة و الأمان، إذا
+
+347
+00:26:23,150 --> 00:26:26,550
+جرح صغير موجود في إيدي أثناء عملية سحب الدم،
+
+348
+00:26:26,550 --> 00:26:31,350
+معناها أنا هيك أذيت نفسي، تمام؟ فبالتالي عشان هيك
+
+349
+00:26:31,350 --> 00:26:35,210
+أنا براعي بتعامل مع إنه أنا بصحى بعينة الدم إن
+
+350
+00:26:35,210 --> 00:26:39,880
+إنها هي عينة معديةو بكون لابسة ال gloves ال .. ال
+
+351
+00:26:39,880 --> 00:26:45,440
+guanti التضية و بستخدم الكحول للتعقيم و بحافظ على
+
+352
+00:26:45,440 --> 00:26:50,240
+نضافة المكان لحتى ما أنقل لنفسي العدوى، تمام؟
+
+353
+00:26:50,240 --> 00:26:55,060
+الاشي مش مخيف و ما ان .. يعني انا مش بصدد ان
+
+354
+00:26:55,060 --> 00:27:00,050
+اعمللكم تهويل لإلهلأ هين الحمد لله قلنا سنوات في
+
+355
+00:27:00,050 --> 00:27:04,050
+المجال و الحمد لله يعني محافظين على أنفسنا و سهل
+
+356
+00:27:04,050 --> 00:27:07,370
+جدا انتوا تتبعوا الخطوات للسلامة و الأمان و
+
+357
+00:27:07,370 --> 00:27:11,430
+تتحافظوا على أنفسكم ولكن يعني زي ما احنا مابدنا
+
+358
+00:27:11,430 --> 00:27:16,450
+نهور الأمر برضه كمان مابدنا نستصغر الأمور و نستهين
+
+359
+00:27:16,450 --> 00:27:20,610
+فيها فبالتالي نقضي أنفسنايعني فقط عليكم اتباع
+
+360
+00:27:20,610 --> 00:27:25,190
+الخطوات المهمة و اللي بتتعلق بالسلامة و الأمان و
+
+361
+00:27:25,190 --> 00:27:28,330
+الحد من انتشار العدوى و هيك انتوا بتكونوا في ال ..
+
+362
+00:27:28,330 --> 00:27:31,650
+الدائرة الخضراء او في ال .. في ال safe line ان شاء
+
+363
+00:27:31,650 --> 00:27:38,800
+اللهطبعا انا إيش بلزمني؟ بلزمني انه to protect
+
+364
+00:27:38,800 --> 00:27:42,620
+myself to protect the other ال colleagues و ال
+
+365
+00:27:42,620 --> 00:27:50,460
+visitors يعني بدي أحمي زملائي زواري المختبر العمال
+
+366
+00:27:50,460 --> 00:27:55,520
+النظافة المراسلين كل حد بيورد على المختبر أيضا بدي
+
+367
+00:27:55,520 --> 00:27:59,320
+أحميه تمام؟ يعني بدي أحمي البيئة المحيطة فيا و
+
+368
+00:27:59,320 --> 00:28:03,580
+أنظفها و أحاول ان انا يكون بيئتي ..فنضيفة سليمة
+
+369
+00:28:03,580 --> 00:28:07,740
+آمنة لحتى أنه ما أؤذي غيري و أيضا to protect the
+
+370
+00:28:07,740 --> 00:28:10,320
+patient، بدي أحمل patient that نفسه، يعني ما أكون
+
+371
+00:28:10,320 --> 00:28:13,500
+أنا عم بنقل عدوة من مريض إلى آخر و من آخر إلى آخر
+
+372
+00:28:13,500 --> 00:28:18,920
+و هكذاhow to protect yourself كيف بديك تحمي نفسك؟
+
+373
+00:28:18,920 --> 00:28:22,800
+حكينا الإشي إحنا مش بصدد التهويل لكن أيضا ما بدنا
+
+374
+00:28:22,800 --> 00:28:26,760
+نستهين في الأمور فقط علينا اتباع الخطوات المهمة و
+
+375
+00:28:26,760 --> 00:28:32,040
+التعليمات المهمة لحتى نحمي أنفسنا wear gloves and
+
+376
+00:28:32,040 --> 00:28:35,460
+lab coat or change gloves after each patient or
+
+377
+00:28:35,460 --> 00:28:39,710
+when contaminatedأول حاجة بدي أرتدي ال gloves اللي
+
+378
+00:28:39,710 --> 00:28:44,330
+هي لجوانتي الطبية و ال lab coat تبعي و يفضل إن أنا
+
+379
+00:28:44,330 --> 00:28:47,930
+أغير ال gloves بين كل مريض و الآخر يعني كل ما أقع
+
+380
+00:28:47,930 --> 00:28:50,570
+بالمريد يعني أنا سحبت عينة من مريض أجي المريض اللي
+
+381
+00:28:50,570 --> 00:28:54,830
+بعده يفضل إن أنا أشلح ال gloves أتخلص منها و ألبس
+
+382
+00:28:54,830 --> 00:28:59,550
+gloves جديدة طبعا إذا تلوثت أيضا لازم أنا أتخلص
+
+383
+00:28:59,550 --> 00:29:04,710
+منهاwash hands frequently لازم انا اغسل ايدي ب ..
+
+384
+00:29:04,710 --> 00:29:08,990
+ب .. بكثرة يعني انا مثلا بين مريض و اخر او بين ..
+
+385
+00:29:08,990 --> 00:29:13,330
+بين .. بين فت .. من فترة الى اخره يفضل ان انا اغسل
+
+386
+00:29:13,330 --> 00:29:16,250
+ايدي اغسل ايدي اول ما افوت المختبر و قبل ما اخرج
+
+387
+00:29:16,250 --> 00:29:20,430
+من المختبر او اغادر المختبر بين كل فترة و تانية
+
+388
+00:29:20,430 --> 00:29:23,350
+مثلا بين كل مريضين تلاتة اربعة انا ب .. بفضل ان
+
+389
+00:29:23,350 --> 00:29:27,520
+انا اروح اغسل ايدي و ارجعتمام؟ على أساس أنه هيك
+
+390
+00:29:27,520 --> 00:29:31,980
+أنا بكون عم بحمي حالي تمام؟ عم بحمي حالي dispose
+
+391
+00:29:31,980 --> 00:29:35,780
+of items in appropriate container يعني أنه أنا لما
+
+392
+00:29:35,780 --> 00:29:40,960
+بدي أتخلص من أدوات السحب تمام؟ dispose items يعني
+
+393
+00:29:40,960 --> 00:29:44,520
+أتخلص من الأدوات و الأجزاء و المكونات اللي .. و
+
+394
+00:29:44,520 --> 00:29:47,620
+الأدوات اللي بشتغل فيها in appropriate container
+
+395
+00:29:47,620 --> 00:29:52,260
+يعني مثلا اللي بدي أنحط في سلةالسلة العادية لازم
+
+396
+00:29:52,260 --> 00:29:55,120
+أنا أطلفه في السلة العادية اللي بده ينحط في السلة
+
+397
+00:29:55,120 --> 00:29:58,840
+اللي محطوط عليها إشارة ال biohazard اللي لازم تفوت
+
+398
+00:29:58,840 --> 00:30:02,740
+على جهازي ال autoclave التعقيم بحطه و بطلفه فيها
+
+399
+00:30:02,740 --> 00:30:07,300
+الأدوات ال sharp و الحاد ده و الإبر و الشوفر
+
+400
+00:30:07,300 --> 00:30:12,620
+بستخدم .. لازم أطلفها في ال sharp boxطبعا؟ فكل إشي
+
+401
+00:30:12,620 --> 00:30:16,640
+لازم أنا أتخلص منه في ال container المخصص لإله
+
+402
+00:30:16,640 --> 00:30:22,500
+لحتى أحمي نفسي إذا .. إذا أنا إجيت مثلا رميت مثلا
+
+403
+00:30:22,500 --> 00:30:27,980
+الشفرة أو الإبرة على ال .. ال bench تبعي فممكن
+
+404
+00:30:27,980 --> 00:30:33,040
+يعني بحركة عشوائية أو أنا مش منتبهة أكون جرحت نفسي
+
+405
+00:30:33,040 --> 00:30:36,160
+طبعا؟ و هذه ال .. ال needle يعني ممكن الإبرة تكون
+
+406
+00:30:36,160 --> 00:30:41,970
+ملوثةفلازم أنا أتخلص منها في ال container المناسب
+
+407
+00:30:41,970 --> 00:30:46,370
+لإلها dispose of needles immediately upon removal
+
+408
+00:30:46,370 --> 00:30:51,010
+from patient vein ال needle اللي هي الإبرة تمام
+
+409
+00:30:51,010 --> 00:30:56,630
+لازم أنا أتخلص منها على طول مجرد ما أنا أطلعها من
+
+410
+00:30:56,630 --> 00:31:01,250
+وريد المريض تمام لإنها صارت ملوثة بدمه و أنا ما
+
+411
+00:31:01,250 --> 00:31:05,030
+بعرف هو دمه يعني نظيف أو غير نظيف لازم أنا أتعامل
+
+412
+00:31:05,030 --> 00:31:09,670
+معاه على إن هو معديكل الأحوال حتى لو أنا كنت عارفة
+
+413
+00:31:09,670 --> 00:31:12,950
+أنه هذا المريض سليم، يعني لو كان هذا المريض بيخصني
+
+414
+00:31:12,950 --> 00:31:16,750
+وعارف أنه سليم ومافيش عنده فيروسات ومافيش عنده أش
+
+415
+00:31:16,750 --> 00:31:22,090
+.. يعني organism .. يعني ميكروبات معضية في دمه،
+
+416
+00:31:22,090 --> 00:31:25,750
+أنا بدي أتعامل مع دمه أن هو معضي، حتى لو أنا ضمن
+
+417
+00:31:25,750 --> 00:31:31,550
+أنه نظيف، تمام؟طيب فأول ما بتطلع ال needle و بتخلص
+
+418
+00:31:31,550 --> 00:31:37,430
+منها لإنها بتكون تلوثت بدمه طبعا في عندي قاعدة راح
+
+419
+00:31:37,430 --> 00:31:41,430
+تتكرر كتير في المختبرات و بتيجي فيها جمل الصح و
+
+420
+00:31:41,430 --> 00:31:47,530
+الغلط don't bend, break or recap or ..طبعا
+
+421
+00:31:47,530 --> 00:31:50,890
+recapped needles to avoid accident needle puncture
+
+422
+00:31:50,890 --> 00:31:54,890
+or splashing of content طبعا أنا مش لازم الإبرة
+
+423
+00:31:54,890 --> 00:31:58,990
+اللي أنا بتطلعها من وريض المريض مش لازم أتنيها
+
+424
+00:31:58,990 --> 00:32:04,690
+أكسرها أو أجرب أحاول أرجع أغطيها مرة تانية علشان
+
+425
+00:32:04,690 --> 00:32:10,550
+ما .. يعني أتجنب إنها هي تنخزني تمام؟ ليش؟ لإن هي
+
+426
+00:32:10,550 --> 00:32:14,030
+ممكن تكون ملوثة بدم المريض وإحنا حكينا إن دم
+
+427
+00:32:14,030 --> 00:32:17,000
+المريض ممكن يكون فيه فيروسات أو أي أنيany
+
+428
+00:32:17,000 --> 00:32:22,240
+infectious microorganism أي مايكروب معدي فإذا أنا
+
+429
+00:32:22,240 --> 00:32:25,260
+نخزف نفسي فيها معناها أنا حقنت هذا ال microbe اللي
+
+430
+00:32:25,260 --> 00:32:30,060
+موجود في دم المريض في جسمي يعني أنا اتسببت بالعدوى
+
+431
+00:32:30,060 --> 00:32:36,460
+لنفسي فإذا never ever never ever never ever bend,
+
+432
+00:32:36,620 --> 00:32:43,190
+break, recap, needle تمام؟ممنوع أحاول أتنيها،
+
+433
+00:32:43,190 --> 00:32:48,730
+أكسرها أو أرجع أغطيها مرة تانية، تمام؟ على طول
+
+434
+00:32:48,730 --> 00:32:53,790
+بفكها من عن ال syringe و بتخلص منها في ال sharp
+
+435
+00:32:53,790 --> 00:32:58,970
+box زي ما هيclean up any blood spill with a
+
+436
+00:32:58,970 --> 00:33:04,470
+disinfectant such as freshly made 10% bleach هلأ
+
+437
+00:33:04,470 --> 00:33:09,750
+طبعا إذا يعني نكب قطرات من الدم على ال bench أو
+
+438
+00:33:09,750 --> 00:33:13,670
+على الأرض أو على أي مكان على أي سطح داخل المختبر
+
+439
+00:33:13,670 --> 00:33:21,310
+لازم أنا أنظفه ب ..ب disinfectant بمعقم تمام و هذا
+
+440
+00:33:21,310 --> 00:33:24,950
+ال disinfectant يعني ممكن يكون كحول ممكن يكون عشرة
+
+441
+00:33:24,950 --> 00:33:28,290
+في المية منظف يعني عشرة في المية مثلا chlorine
+
+442
+00:33:28,290 --> 00:33:31,950
+عشرة في المية iod عشرة في المية ditol عشرة في
+
+443
+00:33:31,950 --> 00:33:35,170
+المية سابون أي محلول سابوني أو منظف موجود داخل
+
+444
+00:33:35,170 --> 00:33:37,670
+المختبر تمام احنا بنستخدم عشرة في المية chlorine
+
+445
+00:33:37,670 --> 00:33:42,710
+فهي يعني و أحيانا بنستخدم .. و بنستخدم الكحول و
+
+446
+00:33:42,710 --> 00:33:45,450
+بنستخدم ال ditol أيضا في عملية ال disinfection
+
+447
+00:33:45,450 --> 00:33:50,930
+اللي هي التعقيمطيب if you stick yourself with
+
+448
+00:33:50,930 --> 00:33:54,750
+contaminated needle إذا إن صار لسمح الله و نخست
+
+449
+00:33:54,750 --> 00:33:58,910
+نفسك ب contaminated needle بإبرة ملوثة إيش بدنا
+
+450
+00:33:58,910 --> 00:34:04,570
+نعمل؟ أول حاجة remove your gloves تمام؟ لازم أنا
+
+451
+00:34:04,570 --> 00:34:09,210
+أرفع ال gloves يعني أشلح ال gloves و أتخلص منهم
+
+452
+00:34:09,210 --> 00:34:15,880
+بالسلة تمام؟بعد هيك بدي أعمل sequencing لل
+
+453
+00:34:15,880 --> 00:34:19,840
+puncture site to promote bleeding، بدي أعصر منطقة
+
+454
+00:34:19,840 --> 00:34:24,860
+ال puncture، يعني بدي أضغط عليها لحتى أحفظ أنه
+
+455
+00:34:24,860 --> 00:34:28,300
+يصير في عندى bleeding، بعد هيك بدي .. يعني هيك
+
+456
+00:34:28,300 --> 00:34:33,150
+بيكون الدم هو عم بيطلعتمام و عم بيسيرله bleeding و
+
+457
+00:34:33,150 --> 00:34:38,110
+بتلع من الجارح اللي أنا نخست نفسي فيه هيك بيكون عم
+
+458
+00:34:38,110 --> 00:34:41,990
+بيعمل wash لمكان الجارح يعني في احتمال كبير انه ال
+
+459
+00:34:41,990 --> 00:34:47,670
+microbe اللي فيها تطلع wash the area well with
+
+460
+00:34:47,670 --> 00:34:53,500
+soup and waterبدي أغسل المكان بالمية و الصابون و
+
+461
+00:34:53,500 --> 00:35:00,420
+بدي أسجل اسم المريض و ال ID number عشان أتبع معاه
+
+462
+00:35:00,420 --> 00:35:05,960
+أشوف هذا المريض إيش عنده و إيش راح يصير معايا في
+
+463
+00:35:05,960 --> 00:35:10,720
+المستقبل من ماذا يحمللي المستقبل من مفاجأات متعلقة
+
+464
+00:35:10,720 --> 00:35:14,780
+بهذا المريض و ال .. ال .. النخزة اللي أنا نخزت
+
+465
+00:35:14,780 --> 00:35:22,120
+فيها نفسي من ال needle الملوثة بدعمهمprotect the
+
+466
+00:35:22,120 --> 00:35:27,840
+patient كيف أنا بدي أحمي المريض تمام place blood
+
+467
+00:35:27,840 --> 00:35:35,400
+collection equipment away from patient sorry place
+
+468
+00:35:35,400 --> 00:35:39,280
+blood collection equipment away from patients
+
+469
+00:35:39,280 --> 00:35:43,300
+especially children and psychiatric patients لازم
+
+470
+00:35:43,300 --> 00:35:48,160
+أنا أبعد قدوات سحب الدم عن المريض خصوصا لو كان هذا
+
+471
+00:35:48,160 --> 00:35:52,120
+المريض طفل أو مريض نفسييعني أدوات حادة أنا بستعمل
+
+472
+00:35:52,120 --> 00:35:57,500
+chauffeur بستعمل needles إبر فممكن يكون هذا المريض
+
+473
+00:35:57,500 --> 00:36:00,840
+يعني إذا كان مثلا مريض نفسي psychiatric فبالتالي
+
+474
+00:36:00,840 --> 00:36:04,320
+ممكن يأذي نفسه إذا كان طفل ممكن يلعب فيها من باب
+
+475
+00:36:04,320 --> 00:36:08,260
+الفضول و أيضا يأذي نفسه فبالتالي أنا بدي أبعد هذه
+
+476
+00:36:08,260 --> 00:36:12,620
+الأدوات عن المريضpractice hygiene for the patient
+
+477
+00:36:12,620 --> 00:36:18,380
+protection، بدي أعقم المكان، السطح، أي مكان بيلامس
+
+478
+00:36:18,380 --> 00:36:23,320
+المريض أو بيحط عليه إيدي، لازم أنا أكون معقمة، بدي
+
+479
+00:36:23,320 --> 00:36:27,380
+ألبس gloves و أبدلهم مثل ما حكينا بين كل مريض و
+
+480
+00:36:27,380 --> 00:36:31,180
+الآخر، change them between each patientand wash
+
+481
+00:36:31,180 --> 00:36:36,300
+your hand frequently و أغسل إيديا ب .. باستمرار من
+
+482
+00:36:36,300 --> 00:36:39,460
+فترة لإلى أخرى علشان أيضا كمان أحمي نفسي و أحمي
+
+483
+00:36:39,460 --> 00:36:43,420
+المريض always wear a clean lab coat or gown يعني
+
+484
+00:36:43,420 --> 00:36:48,460
+أنا بدي ألبس على طول ال lab coat طبيعي يكون انظيف
+
+485
+00:36:48,460 --> 00:36:54,380
+ما يكون ملوثو بعد هيك ببدأ بعملية preparation of
+
+486
+00:36:54,380 --> 00:36:59,360
+the puncture site كيف انا اسحب او احضر مكان السحب
+
+487
+00:36:59,360 --> 00:37:05,300
+سواء كان vein puncture او skin puncture بدي اعقم
+
+488
+00:37:05,300 --> 00:37:11,920
+المكان باستخدام 70% isopropanol طبعا 70%
+
+489
+00:37:11,920 --> 00:37:16,860
+isopropanol يا بنات او تمام مش شرط يعني 70%
+
+490
+00:37:16,860 --> 00:37:23,920
+isopropanolممكن يكون 70% isopropanol or ethanol،
+
+491
+00:37:23,920 --> 00:37:29,000
+طبعا؟ يعني 70% isopropanol أو 70% ethanol نفس
+
+492
+00:37:29,000 --> 00:37:31,800
+الاشي، طبعا؟ نفس الاشي
+
+493
+00:37:34,230 --> 00:37:41,910
+تمام اذا انا بعقم المكان بامسحه ب 70% ethanol و
+
+494
+00:37:41,910 --> 00:37:48,510
+طبعا بعد ما انا اعقم لازم المنطقة اللي عقمتها
+
+495
+00:37:48,510 --> 00:37:53,030
+اتركها جافة يعني اترك مجال لل .. للكحول يتبخر او
+
+496
+00:37:53,030 --> 00:37:59,010
+اني امسح .. ارجع امسح المنطقة ب sterile gauze يعني
+
+497
+00:37:59,010 --> 00:38:05,220
+بشاش او بقطن معقمو يجاف لحتى أنشف المكان أجففه
+
+498
+00:38:05,220 --> 00:38:13,720
+عشان يكون هيك صار انظيف معقم و بالتالي جاهز لسحب
+
+499
+00:38:13,720 --> 00:38:19,620
+العينة بمجرد يا بنات أنه أنا أكون عقمت المكان سواء
+
+500
+00:38:19,620 --> 00:38:26,760
+كان منطقة الوريد أو ال ..ال skin، الإصبع، يعني
+
+501
+00:38:26,760 --> 00:38:29,800
+سواء من الوريد، يعني من منطقة ال .. ال .. اللي هي
+
+502
+00:38:29,800 --> 00:38:34,840
+عندى المفصل، الكوع أو من منطقة ال .. ال .. الأصابع
+
+503
+00:38:34,840 --> 00:38:40,200
+فبمجرد ما أعاقمها ممنوع ألمسها مرة تانية بإيدي
+
+504
+00:38:40,200 --> 00:38:45,060
+لإنه هيك أنا بكون عم بلوسها مرة تانية، احنا في
+
+505
+00:38:45,060 --> 00:38:49,420
+ميكروبات بتكون موجودة على جسمنا و هاي الميكروبات
+
+506
+00:38:49,420 --> 00:38:53,490
+ممكن تكون .. ماتكونش ممرضة على ال skin تبعناطبعا،
+
+507
+00:38:53,490 --> 00:38:57,250
+لكن إذا فاتت للدم، إذا أنا لوثت فيها منطق السحب
+
+508
+00:38:57,250 --> 00:39:00,790
+الدم و فاتت للدم، ممكن تصير ممرضة في داخل الدم،
+
+509
+00:39:00,790 --> 00:39:04,730
+طبعا، فبالتالي لازم .. عشان هيك أنا عم بعقم منطقة
+
+510
+00:39:04,730 --> 00:39:06,550
+ال skin قبل السحب
+
+511
+00:39:12,960 --> 00:39:18,080
+طيب نيجي لأ ال .. ال micro sample technique micro
+
+512
+00:39:18,080 --> 00:39:21,400
+sample يا بنات يعني أنه أنا بدي أحصل على عينة
+
+513
+00:39:21,400 --> 00:39:27,260
+صغيرة الحجم يعني أنا بدي أسحب عينة من ال skin طبعا
+
+514
+00:39:27,260 --> 00:39:31,340
+is .. refers to blood collection by skin puncture
+
+515
+00:39:31,340 --> 00:39:36,240
+يعني أنا بدي أسحب عين الدم من خلالال skin puncture
+
+516
+00:39:36,240 --> 00:39:40,720
+طبعا من خلال أن أنا أحصل أعمل نخزة في ال skin في
+
+517
+00:39:40,720 --> 00:39:43,960
+الإصبع يعني مثلا أو في كعب الرجل عند المريض عند
+
+518
+00:39:43,960 --> 00:39:47,400
+الأطفال حسب وين المكان المهم في ال skin مش في
+
+519
+00:39:47,400 --> 00:39:52,140
+منطقة وريدand is frequently used in the following
+
+520
+00:39:52,140 --> 00:39:56,800
+types of patients طبعا يا بنات هذه العينة وهذا هذا
+
+521
+00:39:56,800 --> 00:40:01,800
+موضوع سؤال امتحان يعني انا امتى او من مين او ايش
+
+522
+00:40:01,800 --> 00:40:06,700
+مين هم المرضى اللي انا بضطر اسحب منهم micro sample
+
+523
+00:40:06,700 --> 00:40:10,820
+technique او بضطر اسحب منهم عينة من خلال skin
+
+524
+00:40:10,820 --> 00:40:14,440
+puncture يعني مابقدر اسحبلهم vein puncture بسحبلهم
+
+525
+00:40:14,440 --> 00:40:18,740
+skin puncture اول اشي الأطفال ال infant حديثي
+
+526
+00:40:18,740 --> 00:40:24,120
+الولادةأقل من 6 شهور أطفال يعني مولودين موليد أقل
+
+527
+00:40:24,120 --> 00:40:28,180
+من 6 شهور في العمر طبعا ماعندهمش large blood
+
+528
+00:40:28,180 --> 00:40:33,180
+supply ماعندهمش كمية دم كبيرة في جسمهم لإن حجمهم
+
+529
+00:40:33,180 --> 00:40:38,940
+صغير احنا حكينا حجم الدم يعني بيتراوح حسب الكتلة
+
+530
+00:40:38,940 --> 00:40:42,580
+تبع الجسم طب إذا هو طفل صغير معناها حجم الدم عنده
+
+531
+00:40:42,580 --> 00:40:50,040
+أيضا صغيرتمام؟ و ال .. بالتالي بيكون خطر عليه إن
+
+532
+00:40:50,040 --> 00:40:54,660
+أنا أسحب عينة من الوريد، تمام؟ فبضطر أنا أسحبله من
+
+533
+00:40:54,660 --> 00:40:58,260
+ال skin، هذا الطفل إما بنخزه من كعب رجله أو من
+
+534
+00:40:58,260 --> 00:41:03,150
+الإصبع، تمام؟in younger children في الأطفال الأكبر
+
+535
+00:41:03,150 --> 00:41:09,530
+شوي بالعمر و لكن الفحص المطلوب لإلهم محتاج small
+
+536
+00:41:09,530 --> 00:41:14,170
+amount of blood يعني هم صح أطفال أكبر و عندهم
+
+537
+00:41:14,170 --> 00:41:19,230
+blood supply أكبر ولكن أنا محتاجة أصلا للفحص small
+
+538
+00:41:19,230 --> 00:41:23,410
+amount of blood طمام طالما أنا محتاجة small amount
+
+539
+00:41:23,410 --> 00:41:27,730
+of blood ف ال skin puncture هي الخيار تبعي بعمل
+
+540
+00:41:27,730 --> 00:41:32,650
+أسحابلهم من خلال ال skin punctureأو when adult has
+
+541
+00:41:32,650 --> 00:41:37,770
+poor vein لما يكون شخص adult ده لغى كبير يعني عنده
+
+542
+00:41:37,770 --> 00:41:43,310
+أوريدة و عنده كمية دم كافية ولكن ال .. الوريد مش
+
+543
+00:41:43,310 --> 00:41:47,310
+انا .. مش عم .. مش عم بستطيع أو مش عم بقدر انه انا
+
+544
+00:41:47,310 --> 00:41:53,500
+أسحبله عينة من الوريد، تمام؟إيه؟ ليش؟ مثلا ممكن
+
+545
+00:41:53,500 --> 00:41:57,660
+يكون منطقة الوريد صارلها burned يعني انحرقت، عنده
+
+546
+00:41:57,660 --> 00:42:02,120
+حروق يعني في جسمه كلها أو في مغطية حجم كبير من
+
+547
+00:42:02,120 --> 00:42:06,720
+جسمه فبالتالي ده يعني صعب أصل للوريد فبالتالي صعب
+
+548
+00:42:06,720 --> 00:42:11,600
+أن أنا أسحب له عينة من الوريد الناس اللي بتاخد
+
+549
+00:42:11,600 --> 00:42:17,240
+chemotherapy لمرضى ال cancer اللي بيتعالجه بال
+
+550
+00:42:17,240 --> 00:42:24,270
+chemotherapy، بالكماويفبالتالي هدول بيصير عندهم
+
+551
+00:42:24,270 --> 00:42:27,770
+ضعف في الأوردة الأوردة تبعتهم بتصير رفيعة و دقيقة
+
+552
+00:42:27,770 --> 00:42:33,390
+جدا فبالتالي صعب ان انا يعني ا .. ا .. احصل عليها
+
+553
+00:42:33,390 --> 00:42:38,250
+او ادور عليها و صعب ان انا افوت فيها إبرة فبالتالي
+
+554
+00:42:38,250 --> 00:42:41,770
+باخدلهم من ال skin puncture كمان
+
+555
+00:42:51,200 --> 00:42:55,440
+تمام؟ إيش الحاجات اللي .. اللي بتلزمني أو الأدوات
+
+556
+00:42:55,440 --> 00:42:58,260
+اللي بتلزمني علشان أنا أصحب عينة من ال skin
+
+557
+00:42:58,260 --> 00:43:02,420
+puncture؟ أول حاجة بلزمني يعني زي ال reagents و ال
+
+558
+00:43:02,420 --> 00:43:05,920
+equipment أول إشي بلزمني سبعين في المية حكينا
+
+559
+00:43:05,920 --> 00:43:09,980
+isopropyl alcohol يعني isopropanol أو سبعين في
+
+560
+00:43:09,980 --> 00:43:15,250
+المية ethanol the same تمام؟الملزمني sterile goes
+
+561
+00:43:15,250 --> 00:43:21,470
+bad يعني قطن أو شاش معقّم و حتى أحطه على مكان
+
+562
+00:43:21,470 --> 00:43:27,270
+السحب العينى يعني بعد ما أخلص سحب و أزيل ال needle
+
+563
+00:43:27,270 --> 00:43:35,880
+بدي أحطه مكانهابلزمني أيضًا مثلًا ال .. يعني
+
+564
+00:43:35,880 --> 00:43:39,980
+blasters عشان أحطها للمريض أيضًا على مكان السحب
+
+565
+00:43:39,980 --> 00:43:44,340
+gloves و هذه ال gloves أنا في .. عندي تلت أنواع من
+
+566
+00:43:44,340 --> 00:43:48,360
+ال gloves إما مصنوعة من ال latex و هي الأكثر شيوعا
+
+567
+00:43:48,360 --> 00:43:52,540
+أو مصنوعة من ال nitril أو ال vinyl راح نحكي عنهم
+
+568
+00:43:52,540 --> 00:43:56,160
+في ال slide الجاي و نشوف إيش الفرق ما بينهم تمام؟
+
+569
+00:43:57,210 --> 00:44:00,910
+warm to protect the patient and philiptomist طبعا
+
+570
+00:44:00,910 --> 00:44:04,970
+ال gloves بتحمي المريض و بتحمي ال .. ال .. ال
+
+571
+00:44:04,970 --> 00:44:09,070
+أخصائي المختبرات sterile disposable blood Lancet
+
+572
+00:44:09,070 --> 00:44:14,070
+طبعا أنا بالزمني ال .. في ال .. سحب ال skin
+
+573
+00:44:14,070 --> 00:44:16,330
+puncture أو في سحب عينة من ال skin puncture
+
+574
+00:44:16,330 --> 00:44:20,780
+بالزمني ال .. ال Lancet طبعاإيش هي ال lancet اللي
+
+575
+00:44:20,780 --> 00:44:26,160
+هي شفرة مصممة يعني مدببة الطرف مصممة لإحدى ثقوب أو
+
+576
+00:44:26,160 --> 00:44:32,680
+نخز في ال skin لحتى نحصل على عينة دم طبعا latex اي
+
+577
+00:44:32,680 --> 00:44:36,040
+gloves ايش ال latex gloves طبعا مصنوعة من ال
+
+578
+00:44:36,040 --> 00:44:40,260
+rubber طبعا و بتستخدم إما في المجال الطبي أو
+
+579
+00:44:40,260 --> 00:44:43,900
+المجال الصناعي ولكن عندي مشكلة إنها ممكن تسبب
+
+580
+00:44:43,900 --> 00:44:47,840
+حساسية طبعا و هي الأكثر شيوعا طبعا أكتر استخداما
+
+581
+00:44:48,450 --> 00:44:51,910
+اللي هي ال gloves اللي لونها أبيض، تمام؟ ال
+
+582
+00:44:51,910 --> 00:44:55,910
+nitrale gloves طبعا مافيش فيها latex، يعني مافيش
+
+583
+00:44:55,910 --> 00:44:58,930
+فيها powder، ال latex يعني فيها powder يا بنات،
+
+584
+00:44:58,930 --> 00:45:02,510
+تمام؟ مصنعة من المطاط و فيها powder من الداخل، ال
+
+585
+00:45:02,510 --> 00:45:05,630
+.. طبعا ال .. ال nitrale gloves مافيش فيها powder،
+
+586
+00:45:05,630 --> 00:45:10,300
+تمام؟مصنعة من ال .. ال .. ال synthetic rubber يعني
+
+587
+00:45:10,300 --> 00:45:13,660
+ال .. المطاط الصناعي مش المطاط الطبيعي، هذه مطاط
+
+588
+00:45:13,660 --> 00:45:20,160
+طبيعي، تمام؟ و فبالتالي هي مابتسببش حساسية، تمام؟
+
+589
+00:45:20,160 --> 00:45:25,740
+و هي puncture resistant يعني مقاومة لإنه يصير ثقب
+
+590
+00:45:25,740 --> 00:45:30,820
+فيهايعني لو ال needle بدها تنخزها عشان انا .. يعني
+
+591
+00:45:30,820 --> 00:45:35,700
+لو صار في accident و بدي أنخز نفسي اذا انا يعني
+
+592
+00:45:35,700 --> 00:45:40,340
+لابسة night rally gloves بيصعب انه يصير في .. ان
+
+593
+00:45:40,340 --> 00:45:44,200
+انا أنخز نفسي medical grade يعني هي مفضلة في
+
+594
+00:45:44,200 --> 00:45:48,140
+المجال الطب يستخدموها في العمليات لونها أزرق و هي
+
+595
+00:45:48,140 --> 00:45:52,670
+بتعطيني درجة حماية أكبر ال vanilla glovesلسه
+
+596
+00:45:52,670 --> 00:45:56,670
+durable يعني لسه protection لسه expensive يعني ما
+
+597
+00:45:56,670 --> 00:46:02,910
+بتتحملش كتير بتعطيني حماية أقل و هي بالتالي أرخصة
+
+598
+00:46:02,910 --> 00:46:07,550
+الثمن مش expensive تمام؟ هذا بالنسبة لأنواع ال
+
+599
+00:46:07,550 --> 00:46:10,530
+gloves تمام؟
+
+600
+00:46:12,170 --> 00:46:19,210
+ال procedure تجربة ال .. ال .. ال skin puncture
+
+601
+00:46:19,210 --> 00:46:24,470
+أول حاجة approach the patient in friendly calm
+
+602
+00:46:24,470 --> 00:46:28,170
+manner provide for their comfort as much as
+
+603
+00:46:28,170 --> 00:46:31,810
+possible and gain the patients cooperation أول
+
+604
+00:46:31,810 --> 00:46:36,130
+حاجة تعاملي مع المريض بشكل friendly و دود و هادق
+
+605
+00:46:36,130 --> 00:46:39,250
+طبعا ما تكوني aggressive معاه ما تكوني عنيفة أو
+
+606
+00:46:39,250 --> 00:46:44,930
+يعني معصبة أو اتوتريةتمام؟ و بدي أكون مريحة لإله
+
+607
+00:46:44,930 --> 00:46:48,530
+إن ان شاء الله ماريح تتقلم كتير و يعني هي نخزة
+
+608
+00:46:48,530 --> 00:46:53,990
+بسيطة و أحاول أريحه يعني لو هو متوتر أخلي يعني هيك
+
+609
+00:46:53,990 --> 00:46:58,870
+ياخد نفس عميق يحاول يرتاح أمسك إيده أعمللها
+
+610
+00:46:58,870 --> 00:47:01,670
+rubbing يعني أفرك إيده أحاول أدفله إيده إذا إيده
+
+611
+00:47:01,670 --> 00:47:07,640
+باردة فبالتالي هيك أنا بكون عم بريحه نفسياand gain
+
+612
+00:47:07,640 --> 00:47:10,580
+the patient cooperation وبالتالي هيك بكون ايضا
+
+613
+00:47:10,580 --> 00:47:14,720
+بخلي الطبيب المريض يكون متعاون معاه اكتر identify
+
+614
+00:47:14,720 --> 00:47:18,060
+the patient correctly بدي اتعرف على المريض بشكل
+
+615
+00:47:18,060 --> 00:47:23,690
+صحيح تمامبدي اسأله انت فلان لفلاني ايش اسمك
+
+616
+00:47:23,690 --> 00:47:29,830
+بالكامل؟ كام عمرك؟ معاك اثبات؟ وين ما .. وين معاك
+
+617
+00:47:29,830 --> 00:47:35,010
+ال kart تبعك؟ اشوف رقمك تطبي؟ انت يعني من ايش
+
+618
+00:47:35,010 --> 00:47:39,390
+بتشكي؟ كذا يعني اسأله كام سؤال احاول اتأكد انه هذا
+
+619
+00:47:39,390 --> 00:47:44,410
+المريض هو يعني المريض الصح لل request الصح عشان
+
+620
+00:47:44,410 --> 00:47:48,820
+احصل على العين الصح تمام؟properly fill out
+
+621
+00:47:48,820 --> 00:47:53,040
+appropriate requisition form طبعا بدي أعبي الجزئية
+
+622
+00:47:53,040 --> 00:47:59,860
+الخاصة فيا من ال request form اللي هي يعنيال ..
+
+623
+00:47:59,860 --> 00:48:04,560
+يعني اللي هي عبارة عن ال time و ال date of sample
+
+624
+00:48:04,560 --> 00:48:08,300
+collection و ال initial طبعا verify the patient
+
+625
+00:48:08,300 --> 00:48:13,280
+condition طبعا إذا كان الفحص محتاج شروط معينة مثلا
+
+626
+00:48:13,280 --> 00:48:18,380
+إنه يكون المريض صايم أو يعني dietary restriction
+
+627
+00:48:18,380 --> 00:48:22,540
+ممتنع عن أكل معينة أو متناول أكل معينة أو ممتنع عن
+
+628
+00:48:22,540 --> 00:48:27,000
+أدوية معينة أو متناول أدوية معينةتوقيت الفحص مثلا
+
+629
+00:48:27,000 --> 00:48:32,000
+في فحصات لازم أعملها في الصبح يعني الصباح المبكر
+
+630
+00:48:32,000 --> 00:48:35,060
+في تمارين .. في فحصات بعملها بعد تمارين رياضية في
+
+631
+00:48:35,060 --> 00:48:39,980
+فحصات بعملها في المساء حسب يعني ال .. ال .. ال
+
+632
+00:48:39,980 --> 00:48:43,480
+time و الظروف المناسبة للفحص بدي أتأكد صايم كام
+
+633
+00:48:43,480 --> 00:48:49,140
+ساعة صومتطيب أكلت كذا إيش الدوة اللي بتاخده ..
+
+634
+00:48:49,140 --> 00:48:53,180
+بتاخد أدوية معينة؟ بدي أسأله على أساس إن أنا أتأكد
+
+635
+00:48:53,180 --> 00:49:00,440
+إن هو مستوى في شروط إجراء الفحص، تمام؟ و طبعا ال
+
+636
+00:49:00,440 --> 00:49:04,940
+medical treatment are all of concern and should be
+
+637
+00:49:04,940 --> 00:49:08,540
+noted on the lab requisition طبعا أي إيش بياخده هو
+
+638
+00:49:08,540 --> 00:49:12,940
+الشخص لازم يكون .. يعني ممنشن إله في ال lab
+
+639
+00:49:12,940 --> 00:49:16,690
+requestposition the patient طبعا بدي اجلس المريض
+
+640
+00:49:16,690 --> 00:49:20,730
+في مكان على كرسي طبعا هذا الكرسي مايكون عالي او
+
+641
+00:49:20,730 --> 00:49:26,430
+مايكونش له دهر مثلا انه هيك بيكون المريض اذا كان
+
+642
+00:49:26,430 --> 00:49:29,610
+الكرسي عالي او مالوش دهر او كذا ممكن يكون المريض
+
+643
+00:49:29,610 --> 00:49:32,650
+دور دلال اذا أغمى عليها على طول بيجي على الأرض
+
+644
+00:49:32,650 --> 00:49:37,120
+وواجهته بتكون محرزةفلأ يعني اللي لازم أنا أجلسه
+
+645
+00:49:37,120 --> 00:49:43,080
+على كرسي و .. أو أخليه يستلقي على سرير و لازم
+
+646
+00:49:43,080 --> 00:49:47,440
+الكرسي هذا إذا كان .. مايكونش عالي و مايكونش ال ..
+
+647
+00:49:47,440 --> 00:49:52,440
+يعني و يكون إيله دهر و أسان و إيله أيدي بحيث أنه ي
+
+648
+00:49:52,440 --> 00:49:56,540
+.. يمسك المريض منيح في حال لو فقده عينه يعني في
+
+649
+00:49:56,540 --> 00:50:01,100
+مرضى بيخافوا، بيتوتروا، في يعني ممكن تصل لدرجة
+
+650
+00:50:01,100 --> 00:50:07,390
+الإغماء من ساحب عين دمlocation of the puncture
+
+651
+00:50:07,390 --> 00:50:12,250
+site طبعا the best location of finger stick ..
+
+652
+00:50:12,250 --> 00:50:16,830
+finger stick أو ال .. يعني
+
+653
+00:50:16,830 --> 00:50:19,730
+ال .. أو ال finger puncture هي نفسها يعني أنا باخد
+
+654
+00:50:19,730 --> 00:50:24,170
+عينة من الإصبع لازم يكونوا يا بنات هو الإصبع
+
+655
+00:50:24,170 --> 00:50:30,260
+التالت و الرابع مين هم التالت و الرابع؟يعني ال ..
+
+656
+00:50:30,260 --> 00:50:35,400
+ال .. ال .. الإصبع الأوسط و اللي .. و اللي بعده،
+
+657
+00:50:35,400 --> 00:50:39,660
+تمام؟ هذا معناها التالت والرابع، يعني الابهام رقم
+
+658
+00:50:39,660 --> 00:50:44,420
+واحد، السبابة رقم اتنين، الأوسط رقم تلاتة، الخنصر
+
+659
+00:50:44,420 --> 00:50:47,500
+اللي هو رقم أربع، اذا هو الأوسط و الخنصر، اللي هو
+
+660
+00:50:47,500 --> 00:50:50,740
+الخنصر لأ، تمام؟ طب ليش أنا اخترت هدول التلات
+
+661
+00:50:50,740 --> 00:50:56,260
+أصابع؟ في عندهم soft tissueكافي بحيث إن أنا لو ..
+
+662
+00:50:56,260 --> 00:51:01,340
+لو نخزت ما بقلمهم و ما بوصل للعظم، طبعا، لو جينا
+
+663
+00:51:01,340 --> 00:51:05,820
+للإبهام، الإبهام بتحسي إصبع مسطح، انفلطح فبالتالي
+
+664
+00:51:05,820 --> 00:51:09,280
+كمية ال soft tissue في منطقة ال .. في المنطقة
+
+665
+00:51:09,280 --> 00:51:14,080
+العلوية فيه جليلة، طبعا، فإذا أنا نخزت ممكن أوصل
+
+666
+00:51:14,080 --> 00:51:19,260
+للعظمفبالتالي بكون أذية العظم طبعا السبابة أنتوا
+
+667
+00:51:19,260 --> 00:51:22,320
+بتعرفوا إحنا بنمسك الجنم باستخدام الإبهام والسبابة
+
+668
+00:51:22,320 --> 00:51:26,980
+والسبابة تبعنا غالبا يعني بيكون rigid أو ناشف شوية
+
+669
+00:51:26,980 --> 00:51:33,080
+طبعا فبرضه كمان ببتعد عنه وفال .. ال .. ال ..
+
+670
+00:51:33,080 --> 00:51:36,320
+الإصبع رقم تلاتة واربعة هما اللي هي فيهم طراوة
+
+671
+00:51:36,320 --> 00:51:39,840
+وفيهم ال soft tissue يعني كفيل بإن أنا أعمل نخزة
+
+672
+00:51:39,840 --> 00:51:44,900
+بعيدة عن العظمالأصبع الصغير هذا الحزين ال .. ال ..
+
+673
+00:51:44,900 --> 00:51:49,180
+ال .. البنصر طبعا صعب إن أنا أعمله نخز لإنه ه ..
+
+674
+00:51:49,180 --> 00:51:55,660
+هأقضيه، تمام؟ هذا بالنسبة للأصابع، التالت والرابع
+
+675
+00:51:55,660 --> 00:51:59,460
+ويفضل إن أنا أستخدم يا بنات ال non-dominant hand
+
+676
+00:51:59,800 --> 00:52:04,140
+تمام؟ يفضل إن أنا أستخدم ال non-dominant hand إيش
+
+677
+00:52:04,140 --> 00:52:07,060
+يعني ال non-dominant hand؟ يعني هو إذا بكتب بال ..
+
+678
+00:52:07,060 --> 00:52:12,180
+بالإيد اليمنى بسحبله من اليسرة إذا هو أعثر بكتب
+
+679
+00:52:12,180 --> 00:52:16,180
+بالإيد اليسرة بسحبله من اليمنى و هكذا don't use
+
+680
+00:52:16,180 --> 00:52:20,180
+the tip of the finger or center of finger تمام؟
+
+681
+00:52:20,180 --> 00:52:26,640
+يمنع أنا استخدام ال .. ال .. رأس الإصبع يعني أو
+
+682
+00:52:26,640 --> 00:52:29,890
+المركز طبعه تمام؟avoid
+
+683
+00:52:32,940 --> 00:52:37,960
+the side of the finger كمان على الجوانب تمام يعني
+
+684
+00:52:37,960 --> 00:52:42,160
+مابس .. مابس بعمل نخزة على الجوانب لإنه هيك أنا
+
+685
+00:52:42,160 --> 00:52:46,120
+بكون .. يعني جربت من منطقة الأضافر و صعب هذه
+
+686
+00:52:46,120 --> 00:52:49,760
+المنطقة أن أنا أعمل لها نخز تمام؟ و أيضا في .. على
+
+687
+00:52:49,760 --> 00:52:53,700
+الجوانب في لسه تشوفها تكون مؤلمة تمام؟ يعني في
+
+688
+00:52:53,700 --> 00:52:58,580
+كمان أيضا تجمع للأعصاب على الجوانب فهذه بتأدي إلى
+
+689
+00:52:58,580 --> 00:53:04,330
+إنه يكون الاشي مؤلمتمام ال .. ال second اللي هو ال
+
+690
+00:53:04,330 --> 00:53:09,390
+.. ال index الأصبع السبابة رقم اتنين هو thicker هو
+
+691
+00:53:09,390 --> 00:53:14,150
+سميك يعني جلده سميك نفسه فصعب أن أنا أعمله نخزا و
+
+692
+00:53:14,150 --> 00:53:20,330
+أصل يعني لعمق معين و أحصل على كمية الدم الكافيةو
+
+693
+00:53:20,330 --> 00:53:23,630
+ال fifth finger الأصبع الصغير الحزين حكينا أنه
+
+694
+00:53:23,630 --> 00:53:28,750
+عنده less soft to show فبالتالي يعني ممكن أنا أصل
+
+695
+00:53:28,750 --> 00:53:33,150
+العظم بسهولة و أنا عم بنخذه فبالتالي أسببله ألم أو
+
+696
+00:53:33,150 --> 00:53:37,030
+damage للبون avoid puncturing a finger which that
+
+697
+00:53:37,030 --> 00:53:39,950
+is cold or cyanotic, swollen, scarred or covered
+
+698
+00:53:39,950 --> 00:53:43,980
+with rashيعني الإصبع اللي شك الإيد لو كانت باردة
+
+699
+00:53:43,980 --> 00:53:47,860
+أو مزرقة ما .. ما .. ما بنخزها لإنه طبعا هي باردة
+
+700
+00:53:47,860 --> 00:53:50,980
+أو مزرقة يا إما من الخوف يا إما من التوتر أو من ..
+
+701
+00:53:50,980 --> 00:53:55,220
+من حالة مرضية عنده يعني مادامها باردة و مزرقة يعني
+
+702
+00:53:55,220 --> 00:53:58,660
+مافيش blood supply كافي فبالتالي يعني أنا لو نخزته
+
+703
+00:53:58,660 --> 00:54:01,600
+مش راح أحصل على كمية دم كافي يعني بس عم بكون بقلم
+
+704
+00:54:01,600 --> 00:54:05,610
+المريض وخلاصswollen ال .. إذا كان هو .. يعني إصبع
+
+705
+00:54:05,610 --> 00:54:09,730
+منتفخ و أنا أجي أنخزه يعني أنا هيك عم بسببله مشكلة
+
+706
+00:54:09,730 --> 00:54:14,050
+إضافية إذا كان فيها scar أو .. أو .. أو عنده rash
+
+707
+00:54:14,050 --> 00:54:22,230
+rash يعني .. يعني ال .. ال .. ال .. الاشي يعني هو
+
+708
+00:54:22,230 --> 00:54:30,750
+الأحمر اللي موجود يعني ال ..ال .. يعني مش عارفة
+
+709
+00:54:30,750 --> 00:54:36,970
+أترجمها بالعربي الصراحة هي ال rush يعني ال .. إذا
+
+710
+00:54:36,970 --> 00:54:40,810
+كان أحيانا بيكون في حساسية و بيكون في إحمرار مع
+
+711
+00:54:40,810 --> 00:54:46,930
+حبوب صغيرة و أشياء هذه بيسميها rush، تمام؟ فيفضل
+
+712
+00:54:46,930 --> 00:54:51,450
+إن أنا ما أستخدمها، تمام؟ إذا كان الإيد فيها rush
+
+713
+00:54:51,450 --> 00:54:55,690
+ما أستخدمها، تمام؟ يعني .. يعني دللت على وجود
+
+714
+00:54:55,690 --> 00:54:58,070
+حساسية أو infection أو التهابات معينة
+
+715
+00:55:01,400 --> 00:55:06,360
+طبعا إذا أنا بدي أسحب عينة من ال heel of the foot،
+
+716
+00:55:06,360 --> 00:55:10,120
+من كعب الرجل، طبعا حاكينا الأطفال ممكن أسحبلهم من
+
+717
+00:55:10,120 --> 00:55:10,860
+كعب الرجل
+
+718
+00:55:14,500 --> 00:55:17,900
+when obtaining blood from infant less than one
+
+719
+00:55:17,900 --> 00:55:22,000
+year إذا كان الطفل أقل من سنة يفضل إن أنا أخدله
+
+720
+00:55:22,000 --> 00:55:25,760
+العينة من كعب رجله، تمام؟ the site chosen should
+
+721
+00:55:25,760 --> 00:55:30,820
+be on the inside medial or outside plantar of the
+
+722
+00:55:30,820 --> 00:55:36,960
+foot يعني هاي .. هاي الرجل، تمام؟ رجل المريض،
+
+723
+00:55:36,960 --> 00:55:42,270
+خليني أكبر ال slideهي هذه رجل المريض تمام؟ هذا ال
+
+724
+00:55:42,270 --> 00:55:47,170
+plantar surface يعني المنطقة الطرفية من القدم
+
+725
+00:55:47,170 --> 00:55:50,730
+بسميها ال plantar surface و هذا ال medial المنطقة
+
+726
+00:55:50,730 --> 00:55:54,750
+الوسطى يعني المحادية للرجل التانية يعني المنطقة
+
+727
+00:55:54,750 --> 00:55:59,030
+الداخلية من الرجل بسميها ال plant .. ال medial
+
+728
+00:55:59,030 --> 00:56:05,000
+surface تمام؟ لو أنا بدي أرسم خطimaginary line
+
+729
+00:56:05,000 --> 00:56:13,500
+تمام خط وهمي ب .. برسمه طبعا من منتصف الأصبع
+
+730
+00:56:13,500 --> 00:56:20,840
+الكبير تمام إلى كعب الرجل و أيضا خط أخر من ال ..
+
+731
+00:56:21,740 --> 00:56:26,080
+المسافة ما بين الإصبع الرابع و الخامس اللي هي هذه
+
+732
+00:56:26,080 --> 00:56:32,700
+المسافة و أيضا برسم خط يتقاطع في ال .. ال .. كعب
+
+733
+00:56:32,700 --> 00:56:37,380
+الرجل تمام؟ أي منطقة في كعب الرجل خارج رأس المتلت
+
+734
+00:56:37,380 --> 00:56:41,940
+بسميها هي المنطقة اللي يعني ممكن أنا أسحب منها عين
+
+735
+00:56:41,940 --> 00:56:48,240
+الدمإذا أول ما بمسك رجل الطفل لأقل من سنة برسم خط
+
+736
+00:56:48,240 --> 00:56:54,900
+وهمي بنظري يمتد من نصف الإصبع الكبير إلى إياش إلى
+
+737
+00:56:54,900 --> 00:56:59,400
+من نصف الإصبع الكبير إلى كعب الرجل ومن أو ال line
+
+738
+00:56:59,400 --> 00:57:04,240
+from between the fourth and the fifth two وخط أيضا
+
+739
+00:57:04,240 --> 00:57:07,780
+من المسافة ما بين الإصبع الرابع والخامس إلى كعب
+
+740
+00:57:07,780 --> 00:57:10,380
+الرجل تمام؟
+
+741
+00:57:11,190 --> 00:57:13,910
+المنطقة هذه اللي في كعب الرجل اللي في رأس المتلات
+
+742
+00:57:13,910 --> 00:57:17,150
+بستثنيها، اذا اي منطقة خارج كأراس المتلات هي
+
+743
+00:57:17,150 --> 00:57:21,370
+المنطقة اللي acceptable for puncture، اذا the area
+
+744
+00:57:21,370 --> 00:57:24,910
+outside of these two lines، المنطقة اللي خارج
+
+745
+00:57:24,910 --> 00:57:31,530
+تقاطع الرأس الخاتين هدولة، يعني خارج رأس المتلات،
+
+746
+00:57:31,530 --> 00:57:35,730
+هذه المنطقة هي acceptable for the puncture، تمام
+
+747
+00:57:36,910 --> 00:57:41,410
+طيب، هلأ ال .. ال puncture دبث، عنق ال puncture،
+
+748
+00:57:41,410 --> 00:57:48,780
+تمام؟يعني أنا بدي .. جدش بدي أفوت عمق في ال .. ال
+
+749
+00:57:48,780 --> 00:57:53,080
+skin اللي أنا عم بنخزه للمريض for finger .. for
+
+750
+00:57:53,080 --> 00:57:59,400
+finger stick لو أنا ماخدة العينة من الإصبع تمام
+
+751
+00:57:59,400 --> 00:58:04,400
+not deeper than 3.1 millimeter يعني ممنوع أفوت
+
+752
+00:58:04,400 --> 00:58:09,730
+أكتر من 3.1 millimeter تمام؟طيب ال distance
+
+753
+00:58:09,730 --> 00:58:14,350
+between the skin surface and bone دايما المسافة ما
+
+754
+00:58:14,350 --> 00:58:18,630
+بين ال .. ال .. ال skin surface ما بين الجلد و
+
+755
+00:58:18,630 --> 00:58:25,730
+العظم بتتراوح ما بين 3.1 ل 10.9 millimeter تمام؟
+
+756
+00:58:25,730 --> 00:58:29,970
+اذا انا لازم ما اصل 3.1 لإن انا ممكن هيك اكون وصلت
+
+757
+00:58:29,970 --> 00:58:34,730
+للعظم لازم اكون وصلت جابل ال 3.1 يعني انا اكون
+
+758
+00:58:34,730 --> 00:58:41,610
+ماكونش فوتت أعمق من 3.1تمام؟ طيب بالنسبة لأ
+
+759
+00:58:41,610 --> 00:58:48,250
+الأطفال اللي يعني عمرهم ست شهور الطفل هذا المسافة
+
+760
+00:58:48,250 --> 00:58:52,950
+ما بين ال skin و العضل ده تروح ما بين واحد و نص
+
+761
+00:58:53,840 --> 00:58:58,220
+millimeter ل اتنين واربعة millimeter تمام فبالتالي
+
+762
+00:58:58,220 --> 00:59:02,800
+يفضل ايش عشان هيك ال finger ما بستخدمه لهدول
+
+763
+00:59:02,800 --> 00:59:06,680
+الأطفال بفضل ان انا استخدملهم heel puncture تمام
+
+764
+00:59:06,680 --> 00:59:11,390
+بستخدملهم او بعمللهم heel punctureتمام اذا بالنسبة
+
+765
+00:59:11,390 --> 00:59:14,450
+ل finger stick عند الناس ال adult او الناس اللى
+
+766
+00:59:14,450 --> 00:59:19,070
+مسموحلى انا اخد منهم من الاصبع مش لازم اتجاوز ال 3
+
+767
+00:59:19,070 --> 00:59:24,430
+.1 mm عمق النخزة علشان لإن المسافة ما بين ال skin
+
+768
+00:59:24,430 --> 00:59:28,920
+و العظم تتراوح ما بين 3.1لعشرة point تسعة
+
+769
+00:59:28,920 --> 00:59:33,840
+millimeter عند الناس طبعا يعني انا مدام مش لازم
+
+770
+00:59:33,840 --> 00:59:36,600
+اصل عمق تلاتة point واحد لإنه في خاطر اكون وصلت
+
+771
+00:59:36,600 --> 00:59:39,940
+العظام لازم اكون وصلت .. العمق يكون قبل التلاتة
+
+772
+00:59:39,940 --> 00:59:46,960
+point واحد تمام؟for heel puncture عمق ال puncture
+
+773
+00:59:46,960 --> 00:59:53,340
+تمام في أي وقت لازم مايكونش اكتر من 2.4 يعني في ال
+
+774
+00:59:53,340 --> 00:59:57,240
+general في الشكل .. بالشكل العام مش لازم يكون اعمق
+
+775
+00:59:57,240 --> 01:00:02,820
+من 2.4 millimeter عشان ا .. يعني اتجنب ان انا اعمل
+
+776
+01:00:02,820 --> 01:00:06,540
+damage لل bonebecause the blood supply for the
+
+777
+01:00:06,540 --> 01:00:10,580
+infant's heel is located between one point يعني
+
+778
+01:00:10,580 --> 01:00:14,440
+الدم موجود يعني الأوعية الدموية الشعيرات الدموية
+
+779
+01:00:14,440 --> 01:00:20,360
+موجودة على بعد من point تلاتة خمسة إلى واحد وستة
+
+780
+01:00:20,360 --> 01:00:24,980
+point millimeter واحد point ستة millimeter تمام
+
+781
+01:00:24,980 --> 01:00:29,360
+تحت ال skin فلازم الضربة تبعتي ما تكونش أعمق من
+
+782
+01:00:29,360 --> 01:00:32,870
+واحد point ستةيعني تكون في .. في العمق ما بين
+
+783
+01:00:32,870 --> 01:00:37,150
+point تلاتة خمسة لواحد point ستة يعني في العمق
+
+784
+01:00:37,150 --> 01:00:40,050
+اللي موجودة فيه الأوعيات الدموية أنا مش لازم أصل
+
+785
+01:00:40,050 --> 01:00:43,490
+أعمق لإنه هيك أكون .. بكون عم بقلم المريض ممكن
+
+786
+01:00:43,490 --> 01:00:48,050
+أوصل للعظم ممكن أوصل لل nerve تبعته فبالتالي أقلمه
+
+787
+01:00:48,050 --> 01:00:53,840
+و .. أو أسببله damage يعني فعال فاضييعني أنا بس
+
+788
+01:00:53,840 --> 01:00:57,640
+محتاجة ضربة قوية عميقة enough .. enough deep تمام
+
+789
+01:00:57,640 --> 01:01:02,880
+بحيث انه يعني يتمكني ان انا أسحب عين الدم تبعتيطيب
+
+790
+01:01:02,880 --> 01:01:05,620
+performance of skin puncture مجرد ما أنا عملت
+
+791
+01:01:05,620 --> 01:01:09,040
+التحضيرات هذه و عرفت من وان بدي أسحب العينة تمام؟
+
+792
+01:01:09,040 --> 01:01:12,040
+هلأ بدي أعمل ال .. ال .. ال performance of skin
+
+793
+01:01:12,040 --> 01:01:15,380
+puncture the puncture site should be .. shouldn't
+
+794
+01:01:15,380 --> 01:01:18,520
+be cold طبعا مش لازم يكون بارد ولا cyanotic ولا
+
+795
+01:01:18,520 --> 01:01:25,220
+swollen ولا فيه rash ال .. عليه يعني احمرار أو
+
+796
+01:01:25,220 --> 01:01:32,020
+حساسية أو انتفاخ أو أي إشي تمام؟must be warmed
+
+797
+01:01:32,020 --> 01:01:36,460
+either by rubbing the side, hanging the hand or
+
+798
+01:01:36,460 --> 01:01:43,000
+applying hot gauze in venture side هلأ شوفوا معايا
+
+799
+01:01:43,000 --> 01:01:47,340
+طبعا مش لازم المكان يكون بارد وإذا كان بارد أنا
+
+800
+01:01:47,340 --> 01:01:50,000
+لازم أدفيه إما من خلال إن أنا أمسك إيده و أفركها
+
+801
+01:01:50,000 --> 01:01:54,060
+بين إيديا أو أخلي أعملها hanging hand إيش يعني
+
+802
+01:01:54,060 --> 01:01:58,430
+hanging hand؟طبعا، هلأ انت جالسة على كرسي المكتب،
+
+803
+01:01:58,430 --> 01:02:02,470
+إيدك على مستواكي، لو جربت تنزلي إيدك لتحت .. لتحت،
+
+804
+01:02:02,470 --> 01:02:06,710
+يعني باتجاه .. يعني بحيث أنه أصابعك تكون مشيرة إلى
+
+805
+01:02:06,710 --> 01:02:10,030
+الأسفل، إلى الأرض، طبعا، هتلاحظ إنه الأوعية
+
+806
+01:02:10,030 --> 01:02:13,810
+الدموية تبعتك اللي في إيدك، طلعي على الشراين اللي
+
+807
+01:02:13,810 --> 01:02:18,090
+في إيدك، طبعا، هتلاحظ إنها هي برزتارفع إيدك مرة
+
+808
+01:02:18,090 --> 01:02:23,510
+تانية، بتلاحظ أنه اختفى البروز هذا، تمام؟ فارجع
+
+809
+01:02:23,510 --> 01:02:27,490
+نزليها مرة تانية، معناه إذا أنت نزلت إيدك أدنى من
+
+810
+01:02:27,490 --> 01:02:31,130
+مستواكي، معناه أنت سمحت للدم أنه يتضفق في إيلها،
+
+811
+01:02:31,130 --> 01:02:35,670
+على طول بتدفع، تمام؟ أو من خلال أن أنا أاخد شاش
+
+812
+01:02:35,670 --> 01:02:41,350
+دافي أو شاش ساخن و يعني hot goes و أحطه على مكان
+
+813
+01:02:41,350 --> 01:02:46,650
+ال puncture عشان أدفيهطيب use a sterile lancet إذا
+
+814
+01:02:46,650 --> 01:02:49,950
+الشفرة أو الأداة اللى أنا بستخدم فيها بسحب فيها من
+
+815
+01:02:49,950 --> 01:02:54,470
+ال skin puncture باسمها Lancet تمام؟ إيش اسمها؟
+
+816
+01:02:54,470 --> 01:03:00,650
+Lancet make طبعا لازم أنا أعمل puncture دربة أو
+
+817
+01:03:00,650 --> 01:03:05,090
+ثقب من خلال .. من خلال إيش؟ لازم إيه لها صفات هاي
+
+818
+01:03:05,090 --> 01:03:08,890
+الدربة اللى أنا بقدر أعملها؟ تكون strong قوية و
+
+819
+01:03:08,890 --> 01:03:13,190
+fast and deep skin punctureإذا لازم تكون strong و
+
+820
+01:03:13,190 --> 01:03:17,550
+fast و deep مابنفع أنا أكون هيك يعني عم .. عم بحرك
+
+821
+01:03:17,550 --> 01:03:22,530
+إيدي ببطء و .. عشان أجي أنخذ المريض أو إنه يكون ..
+
+822
+01:03:22,530 --> 01:03:25,930
+تكون يدوب خفيفة الضربة لأ يعني انا بكون ممكن ..
+
+823
+01:03:25,930 --> 01:03:29,250
+إذا خفيفة يعني معناها أنا بس خدشت ال skin مافوتش
+
+824
+01:03:29,250 --> 01:03:34,390
+deep enough تمام؟فبالتالي لازم strong, fast, deep
+
+825
+01:03:34,390 --> 01:03:39,370
+تمام؟ و أيضا لازم تكون من خصائصها إنها تكون
+
+826
+01:03:39,370 --> 01:03:43,770
+اشمالها يا بنات perpendicular to the ridges of the
+
+827
+01:03:43,770 --> 01:03:49,030
+fingerprint و عمودية على خطوط البصمة لحظة طلع على
+
+828
+01:03:49,030 --> 01:03:55,560
+أصابعك مخططين بشكل دائري تمام؟ لازم ال ..الضربة
+
+829
+01:03:55,560 --> 01:04:02,100
+تبعتي تكون عمودية على خطوط البصمة وإن شاء الله بس
+
+830
+01:04:02,100 --> 01:04:06,100
+نرجع المختبر ونجرب ال skin puncture راح أورجيكم
+
+831
+01:04:06,100 --> 01:04:11,420
+إيش معنى كلمة عمودية على خطوط البصمة، تمام؟ طيبهذا
+
+832
+01:04:11,420 --> 01:04:15,040
+.. ده أنا .. طبعا ليش أنا بديها عمودية؟ لإنه لو
+
+833
+01:04:15,040 --> 01:04:18,760
+أنا عملت ال .. ال .. الجرح أو ال .. ال .. الثقوب
+
+834
+01:04:18,760 --> 01:04:22,660
+أو ال skin puncture موازي لخطوط البصمة راح يسيل
+
+835
+01:04:22,660 --> 01:04:26,400
+الدم يسيل ويمشي مع خطوط البصمة تمام؟ يمشي مع
+
+836
+01:04:26,400 --> 01:04:30,980
+الخطوط بتلاقي الدم صار سايح فبالتالي ما بيتجمع
+
+837
+01:04:30,980 --> 01:04:35,060
+كقطرات و صعب أنا أجمعهم تمام؟ انا بدي .. لازم يكون
+
+838
+01:04:35,060 --> 01:04:38,800
+الدم عمودي .. ال .. الضربة تكون عمودية على ال ..
+
+839
+01:04:38,800 --> 01:04:44,170
+ال .. ال ..خطوط البصمة فبالتالي هذا بيمكن انه انا
+
+840
+01:04:44,170 --> 01:04:49,930
+.. بيمكنني من انه لما انا يعني يطلع الدم بيطلع على
+
+841
+01:04:49,930 --> 01:04:54,050
+هائت قطارات و يسهل اجمعه داخل الإنبوبة، تمام؟ لكن
+
+842
+01:04:54,050 --> 01:04:58,110
+لو ساح مع خطوط البصمة أو في الفرشات هذه اللي ..
+
+843
+01:04:58,110 --> 01:05:02,610
+اللي موجودة بين الخطوط صعب ان انا اجمعه، هذا معنى
+
+844
+01:05:02,610 --> 01:05:06,950
+انه يكون perpendicular، تمام؟عشان الدم ال drop of
+
+845
+01:05:06,950 --> 01:05:12,050
+blood قطرة الدم ما تروحش run down ما تسيحش أو ما
+
+846
+01:05:12,050 --> 01:05:19,520
+تمشيش مع ال ridges مع خطوط البصمة تمامwipe away
+
+847
+01:05:19,520 --> 01:05:24,940
+the first drop of blood ايوة اول قطرة دم بتطلع بعد
+
+848
+01:05:24,940 --> 01:05:29,560
+ال puncture بدي اتخلص منها which tend to contain
+
+849
+01:05:29,560 --> 01:05:32,500
+excessive tissue fluid طبعا يا بنات انا بما اني
+
+850
+01:05:32,500 --> 01:05:35,740
+انا عملت نخزة في ال skin يعني انا عملت cell damage
+
+851
+01:05:35,740 --> 01:05:39,520
+دمرت خلايا الخلايا دي فيها cytoplasm في حوالها
+
+852
+01:05:39,520 --> 01:05:42,720
+سوائل في extra cellular fluid حوالها اذا ان هي
+
+853
+01:05:42,720 --> 01:05:47,430
+فيها داخلها سائل و مخاطب سائلفالدم هذا اللي بيطلع
+
+854
+01:05:47,430 --> 01:05:52,150
+في أول قطرة بيكون يحتوي على ال tissue fluid السائل
+
+855
+01:05:52,150 --> 01:05:55,290
+اللي موجود هذا داخل الانسجار داخل الخلايا أو محيط
+
+856
+01:05:55,290 --> 01:06:01,090
+فيها يعني بيكون هذا الدم إشمال و مخفف و لا يعبر عن
+
+857
+01:06:01,090 --> 01:06:06,410
+الدم اللي بيجر في الأوردة تبعتنا فبالتالي أنا لازم
+
+858
+01:06:06,410 --> 01:06:10,350
+أتخلص منه عشانك بعملها wipe away بمسحها أول قطر
+
+859
+01:06:10,350 --> 01:06:15,310
+الدم بتطلعبعدين collect drops of blood into the
+
+860
+01:06:15,310 --> 01:06:18,950
+collection device by gently massaging the finger
+
+861
+01:06:18,950 --> 01:06:25,670
+يعني بستخدم طبعا بيكون في عندي قنابيب مخصصة لجمع
+
+862
+01:06:25,670 --> 01:06:33,620
+الدم من ال skin من الإصبع سواء أو من الكعبو طبعا
+
+863
+01:06:33,620 --> 01:06:40,160
+يا بنات يعني كيف دي أحكيلكم إياها؟ يعني مثلا بصير
+
+864
+01:06:40,160 --> 01:06:44,020
+أنا أعمل مساج خفيف، باجي من بداية الإصبع، من عند
+
+865
+01:06:44,020 --> 01:06:47,720
+.. يعني من أول الإصبع، من عند إيدي، تمام؟ بعمل
+
+866
+01:06:47,720 --> 01:06:52,320
+مساج خفيف خفيف و لما بقصل مكان ال puncture، بضغط
+
+867
+01:06:52,320 --> 01:06:57,270
+ضغطةتمام؟ ففي هذه الحالة أنا بكون عم بعمل gentle
+
+868
+01:06:57,270 --> 01:07:02,710
+massaging عشان أحفظ ال .. ال .. يعني سريان الدم و
+
+869
+01:07:02,710 --> 01:07:06,930
+أنه يطلع معايا دم كافي، إذا عصرت كتير يا بنات الدم
+
+870
+01:07:06,930 --> 01:07:10,530
+هيتجلط و هيسكر الجرح و مش هاخد الدم الكافي، مش
+
+871
+01:07:10,530 --> 01:07:14,980
+لازم ..أنا أعصر الدم، أنا بسيب .. بسيب إيد المريض
+
+872
+01:07:14,980 --> 01:07:19,500
+يعني ب .. و ما ب .. بضغط عليها و مجرد إنه أنا بس
+
+873
+01:07:19,500 --> 01:07:23,780
+من بداية أصبعه بصير أعمل مساج خفيف يعني يده بكون
+
+874
+01:07:23,780 --> 01:07:28,160
+أنا عم بلامس أصبعه و مجرد ما أصل مكان الجرح بكون ب
+
+875
+01:07:28,160 --> 01:07:33,060
+.. بضغط ضغطة بطلع الدم، تمام؟ هذا معناه ال .. ال
+
+876
+01:07:33,060 --> 01:07:37,130
+.. ال gentle massagingavoid excessive pressure
+
+877
+01:07:37,130 --> 01:07:43,070
+ممنوع انا اضغط بالحامي ليش؟ اول سبب حكيتلكم انه
+
+878
+01:07:43,070 --> 01:07:47,390
+ممكن يتوقف الدم تاني سبب ممكن انه انا هيك بكون عم
+
+879
+01:07:47,390 --> 01:07:51,290
+بعصر الخلايا اللي انا عملتلها damage و يصير في
+
+880
+01:07:51,290 --> 01:07:54,370
+عندي squeeze لل tissue fluid يعني يطلع ال tissue
+
+881
+01:07:54,370 --> 01:07:59,630
+fluid يساقل الخلايا او عصارة الخلايا تطلع مع الدم
+
+882
+01:07:59,630 --> 01:08:05,230
+و هيك انا بكون باخد دم مخفف تمام؟طيب بعد ما بسحب
+
+883
+01:08:05,230 --> 01:08:09,470
+الكمية الكافية have the patient hold a small gauze
+
+884
+01:08:09,470 --> 01:08:12,710
+bed over the puncture site for a couple of minutes
+
+885
+01:08:12,710 --> 01:08:18,130
+to stop the bleeding يعني بخلي المريض أنه يحط قطعة
+
+886
+01:08:18,130 --> 01:08:22,210
+من القطن أو الشاشة على أصبعه و يضغط عليها لحتى
+
+887
+01:08:22,210 --> 01:08:26,050
+يتوقف النزيف و بتخلص من الأدوات اللي هي ال
+
+888
+01:08:26,050 --> 01:08:28,550
+contaminated material و ال supplies القطن اللي أنا
+
+889
+01:08:28,550 --> 01:08:32,280
+قمت فيه و القطن اللي مسحت فيه أول قطرةال .. ال
+
+890
+01:08:32,280 --> 01:08:35,600
+Lancet اللي استخدمتها لإحداث ال .. ال نخزة أو
+
+891
+01:08:35,600 --> 01:08:40,780
+الثقوب و هذه كلها الأدوات بتخلص منها properly في
+
+892
+01:08:40,780 --> 01:08:44,720
+ال container الخاص فيها مثلا ال Lancet عبارة عن
+
+893
+01:08:44,720 --> 01:08:48,660
+أداة حادة بستخدم .. باطلفها في ال sharp box القطن
+
+894
+01:08:48,660 --> 01:08:51,880
+ملوث بالدم إذا بحطه في السلة الموجود .. م .. م ..
+
+895
+01:08:51,880 --> 01:08:55,760
+محطوط عليها علامة biohazard القطن المش ملوث بالدم
+
+896
+01:08:55,760 --> 01:09:00,060
+بستخ .. باطلفه في السلة العادية تمام؟الورقة
+
+897
+01:09:00,060 --> 01:09:04,020
+المغلفة لا ال Lancet بتلفها بالورقة العادية بالسلة
+
+898
+01:09:04,020 --> 01:09:09,140
+العادية فبالتالي اذا كل اشي لازم اتخلص منه بال
+
+899
+01:09:09,140 --> 01:09:14,020
+container المناسب ليله بالوعاء المناسب ليله label
+
+900
+01:09:14,020 --> 01:09:18,680
+all appropriated tubes at the patient bedside طبعا
+
+901
+01:09:18,680 --> 01:09:22,680
+لازم انا العينة تبعتي اعمللها labeling وانا
+
+902
+01:09:22,680 --> 01:09:27,750
+نستاتني جنب المريضمش يعني انه خلاص يلا اروح و
+
+903
+01:09:27,750 --> 01:09:30,270
+هاتوا اللي بعده و بعدين بعمل laboring، ما انا
+
+904
+01:09:30,270 --> 01:09:34,930
+بنسأ، تمام؟ او ممكن اخربط، فلأ ما اعتمد على
+
+905
+01:09:34,930 --> 01:09:40,470
+ذاكرتي، افضل ذاكرة هي الذاكرة المكتوبة، تمام؟و
+
+906
+01:09:40,470 --> 01:09:45,070
+أكتب مباشرة المعلومات أعمل labeling للعينة
+
+907
+01:09:45,070 --> 01:09:51,170
+بالمعلومات اللازمة يعني الاسم اسمه الأول و عائلته
+
+908
+01:09:51,170 --> 01:09:55,230
+و ال initial of middle name التاريخ الميلاد ال
+
+909
+01:09:55,230 --> 01:09:59,490
+gender ال .. طبعا مثلا مهم يا بنات كمان أعمل
+
+910
+01:09:59,490 --> 01:10:05,820
+labeling لل genderطبعا مثلا في شخص اسمه ضياء طبعا
+
+911
+01:10:05,820 --> 01:10:09,660
+أنا مش عارفة هو ضياء بنت ولا ولد، تمام؟ لازم يكون
+
+912
+01:10:09,660 --> 01:10:13,580
+يعني يميز ان هو male أو female خصوصا مع الأسماء
+
+913
+01:10:13,580 --> 01:10:17,280
+اللي تصلح لإنه يكون ل male أو female زي ضياء، نور،
+
+914
+01:10:17,280 --> 01:10:21,760
+إسلام زي أشياء .. الأشياء هاد .. الأسماء هادى، مهم
+
+915
+01:10:21,760 --> 01:10:25,100
+إن أنا يعني أو مع كل الأسماء مهم إن أنا أميز ال
+
+916
+01:10:25,100 --> 01:10:28,720
+gender of patientdelivers specimen promptly to the
+
+917
+01:10:28,720 --> 01:10:33,900
+laboratory فورا لازم انا اسلم العينة للمختبر
+
+918
+01:10:33,900 --> 01:10:38,920
+لاجراء الفحوصات اللازمة اذا بحدد الاصبع المراد
+
+919
+01:10:38,920 --> 01:10:43,560
+اجراء skin puncture لاقله تمام لازم يكون التالت او
+
+920
+01:10:43,560 --> 01:10:47,100
+الرابع هي الاول الابهام السبابة التاني هي هنا
+
+921
+01:10:47,100 --> 01:10:51,880
+التالت الوسطىو هي الرابع و الاخير الخامس طبعا هو
+
+922
+01:10:51,880 --> 01:10:57,040
+الوسطى و الخنصر هم اللي باستخدمهم او يعني هو
+
+923
+01:10:57,040 --> 01:11:00,560
+التالت و الرابع يعني رقم تلاتة الوسطى و اللي بعده
+
+924
+01:11:00,560 --> 01:11:05,700
+هدول اللي مناسبين طبعا بحضر الأدوات تبعتي بفتح ال
+
+925
+01:11:05,700 --> 01:11:10,680
+Lancetطبعا و بعقمها يا بنات بعقم المكان بالكحول
+
+926
+01:11:10,680 --> 01:11:13,520
+بفتح ال Lancet تبعتي ال Lancet زي ما انتوا شايفين
+
+927
+01:11:13,520 --> 01:11:18,100
+شفرة مدبّبة الطرف طبعا إلها طرف مدبّب فأنا بفو ..
+
+928
+01:11:18,100 --> 01:11:22,740
+بعمل ضربة بحيث إن هاي الضربة تكون strong, fast,
+
+929
+01:11:23,420 --> 01:11:26,700
+deep and perpendicular الأربع خواص طبيعتها اللي
+
+930
+01:11:26,700 --> 01:11:32,090
+بتيجي أسألكم عليها بالامتحانتمام بعدين بصحى بتخلص
+
+931
+01:11:32,090 --> 01:11:35,450
+من أول قطر الدم بعدين ببدأ من هنا من عند بداية
+
+932
+01:11:35,450 --> 01:11:40,090
+الإصبع بعمل gentle massage المساج خفيف جدا و لما
+
+933
+01:11:40,090 --> 01:11:44,310
+أصل لمنطقة النخزة بضغط ضغطة تمام مجرد ما أضغط ضغطة
+
+934
+01:11:44,310 --> 01:11:48,910
+.. ضغطة تمام بيبدأ تطلع عندي تتشكل قطرة دائرية
+
+935
+01:11:48,910 --> 01:11:53,790
+بجمعها في ال tube المخصص لجمع العينات و بعد هيك
+
+936
+01:11:53,790 --> 01:11:57,270
+ايش بس برسل ال .. بعمل labeling للعينة مباشرة و
+
+937
+01:11:57,270 --> 01:12:01,650
+برسلها للمختبربعطي المريض قطنة او شاشة او قطعة
+
+938
+01:12:01,650 --> 01:12:06,010
+معقمة ان هو يضغط على المكان الجرح لحين التوقف
+
+939
+01:12:06,010 --> 01:12:09,090
+النظيف تمام؟ انت شايفين القطرة كيف .. القطرة كيف
+
+940
+01:12:09,090 --> 01:12:13,290
+اتكون دائرية لإن انا فوتت عمودية على ال ridges
+
+941
+01:12:13,290 --> 01:12:18,490
+تمام؟ و هحكيلكم اش يعني عمودية في داخل المختبر ان
+
+942
+01:12:18,490 --> 01:12:21,670
+شاء الله تمام
+
+943
+01:12:24,170 --> 01:12:29,030
+طيب خلينا بس ال .. ال .. ناخد المناقشة أو الجزء
+
+944
+01:12:29,030 --> 01:12:34,730
+المتعلق بال .. السحب
+
+945
+01:12:34,730 --> 01:12:39,210
+.. العينة الدم من ال skin طبعا يا بنات العينة ال
+
+946
+01:12:39,210 --> 01:12:42,190
+skin puncture أو الدم اللي جاى من ال skin puncture
+
+947
+01:12:42,190 --> 01:12:47,290
+هو مش دم وريدي ولا شرياني هو عبارة عن mix مندم
+
+948
+01:12:47,290 --> 01:12:51,190
+شرياني ووريدي
+
+949
+01:12:51,190 --> 01:12:55,130
+ومن capillary tubes، تمام؟ ويعني حتى كمان من
+
+950
+01:12:55,130 --> 01:12:59,890
+الشعيرات الدموية فبالتالي التركيز داخل هذا الدم
+
+951
+01:12:59,890 --> 01:13:03,590
+بتكون مختلفة مثلا تركيز ال .. ال .. ال analytes أو
+
+952
+01:13:03,590 --> 01:13:07,830
+المواد المرادقية أو ال .. ال .. ال .. الأشياء
+
+953
+01:13:07,830 --> 01:13:11,150
+المرادقياسها سواء كانت أنا بدأ أقيس بروتينات،
+
+954
+01:13:11,150 --> 01:13:15,060
+هرموناتglucose، دهون، أي إشي بدي أقيصه في .. في
+
+955
+01:13:15,060 --> 01:13:19,300
+العينة هذه تمام دي أجري له فحص لازم .. بيكون مختلف
+
+956
+01:13:19,300 --> 01:13:24,380
+طبعا تركيزه داخلها عن تركيزه في الوريد لإنه هذا
+
+957
+01:13:24,380 --> 01:13:28,120
+عبارة عن mixture، مزيج من الدم الوريدي والشرياني
+
+958
+01:13:28,120 --> 01:13:34,500
+وحتى الشعيرات الدموية فبالتالي لذلك لازم أنا أ
+
+959
+01:13:34,500 --> 01:13:41,470
+mention أكتب في ال ..ال report في النتيجة في
+
+960
+01:13:41,470 --> 01:13:46,030
+التقرير اللي بيطلع من المختبر النتيجة احكي انه هذه
+
+961
+01:13:46,030 --> 01:13:52,930
+النتيجة اللي فحص أجري على عينة was obtained from
+
+962
+01:13:52,930 --> 01:13:57,890
+skin puncture تمام؟ اذا تركيز مختلفة بتكون و ال
+
+963
+01:13:57,890 --> 01:14:01,860
+differbetween skin puncture blood and arterial or
+
+964
+01:14:01,860 --> 01:14:05,960
+venous يعني بتركيز مختلفة في هذا الدم اللي جامل ال
+
+965
+01:14:05,960 --> 01:14:09,260
+skin puncture عن التركيز اللي موجودة في الدم
+
+966
+01:14:09,260 --> 01:14:16,120
+الوليدي او الشرياني فبالتاليلازم انه طبعا في .. في
+
+967
+01:14:16,120 --> 01:14:20,340
+ال test report في التقرير should indicate يعني
+
+968
+01:14:20,340 --> 01:14:25,680
+أشير إلى أنه العينة الدم هذه حصلنا عليها من skin
+
+969
+01:14:25,680 --> 01:14:29,060
+puncture عشان الدكتور لما يقيم وضع المريض ياخد
+
+970
+01:14:29,060 --> 01:14:32,520
+بعين الاعتبار أنه العينة هي skin puncture يعني
+
+971
+01:14:32,520 --> 01:14:36,380
+التركيز مختلفة شويةexcessive massaging لو انا بدي
+
+972
+01:14:36,380 --> 01:14:40,640
+اعمل ال massage القوي ايش حكيناه اول حاجة بكون عم
+
+973
+01:14:40,640 --> 01:14:44,020
+بعمل squeeze تعصير ل ال finger او ال foot او اللي
+
+974
+01:14:44,020 --> 01:14:48,080
+هو المكان ل skin puncture فبالتالي بكون بطلع عصارة
+
+975
+01:14:48,080 --> 01:14:51,820
+الخلايا وبالتالي بخفف الدم بيصير عندى diluted
+
+976
+01:14:51,820 --> 01:14:58,050
+bloodأو أحيانا ممكن يسبب تجلط للدم سريع وبالتالي
+
+977
+01:14:58,050 --> 01:15:01,250
+توقف خروج الدم يعني انا مابحصل على ال volume
+
+978
+01:15:01,250 --> 01:15:05,910
+المناسب يا بحصل على الدم مخفف فبالتالي مش لازم انا
+
+979
+01:15:05,910 --> 01:15:09,570
+اعمل excessive massaging لأ gentle massaging
+
+980
+01:15:09,570 --> 01:15:17,870
+وبالتالي انه يعني بيكون هيك مناسب لاستمرارية ضمن
+
+981
+01:15:17,870 --> 01:15:22,130
+استمرارية انه خروج الدم وانه انا بحصل على دم مش
+
+982
+01:15:22,460 --> 01:15:26,380
+المخافة Since deep puncture is not any more
+
+983
+01:15:26,380 --> 01:15:30,400
+painful than superficial puncture it's best to go
+
+984
+01:15:30,400 --> 01:15:34,000
+deep enough the first time thereby avoid
+
+985
+01:15:34,000 --> 01:15:37,560
+puncturing the patient a second time يعني ال deep
+
+986
+01:15:37,560 --> 01:15:41,560
+puncture بتكونش مقليمة زي ال superficial فبالتالي
+
+987
+01:15:41,560 --> 01:15:45,980
+أنا لازم أجوجلبي و أعمل puncture أعمل ضربة قوية و
+
+988
+01:15:45,980 --> 01:15:51,120
+تكون عميقة enoughتمام؟ عشان اتجنب ان انا انخذ
+
+989
+01:15:51,120 --> 01:15:53,700
+المريض مرة تانية لو طلعت superficial بدي ارجع
+
+990
+01:15:53,700 --> 01:15:57,160
+انخذه مرة تانية وهذا non-ethical مش أخلاقي ان انا
+
+991
+01:15:57,160 --> 01:16:01,290
+اضل اجرب المريض يعني انا بكون عم بقلمهexcess
+
+992
+01:16:01,290 --> 01:16:07,110
+crying إذا كان المريض هذا متوتر و يعني ببكي إذا
+
+993
+01:16:07,110 --> 01:16:12,170
+كان خصوصا طفل هذا راح يأثر على نتائج المختبر يعني
+
+994
+01:16:12,170 --> 01:16:15,170
+مثلا ممكن يرفع ال white pieces نتيجة ال stress
+
+995
+01:16:15,170 --> 01:16:18,050
+يعني ممكن ال white blood cell count يكون يصير له
+
+996
+01:16:18,050 --> 01:16:23,830
+increase falsely increase فيعني يزيد و هو زيادة
+
+997
+01:16:23,830 --> 01:16:28,780
+خاطئة يعني إنه بس نتيجة التوترفبالتالي أنا الأفضل
+
+998
+01:16:28,780 --> 01:16:32,940
+أنه هذا المريض أستنى عليه نص ساعة على ساعة بعد ما
+
+999
+01:16:32,940 --> 01:16:37,720
+يخلص يعني موجة البكاء اللي هو فيها أحاول أهدي
+
+1000
+01:16:37,720 --> 01:16:40,140
+أستنى عليه من نص ساعة على ساعة و بعدين أسحب له
+
+1001
+01:16:40,140 --> 01:16:45,240
+العينة طبعا ال thumb وال .. وال .. ال .. يعني
+
+1002
+01:16:45,240 --> 01:16:49,410
+الإصبع الكبيرال .. الإبهام و الإصبع الكبير بالرجل
+
+1003
+01:16:49,410 --> 01:16:53,150
+و ال ear loop اللي هي حلمة ال .. ال .. الأذن أو
+
+1004
+01:16:53,150 --> 01:16:56,910
+يعني طرف .. طرف الأذن مكان ما بنحط ال .. ال ..
+
+1005
+01:16:56,910 --> 01:17:01,230
+الحلق يعني هذا طبعا صعب يا بنات نعمله skin
+
+1006
+01:17:01,230 --> 01:17:05,070
+puncture ممنوع يعني مابستخدمله ما .. يعني مابعمله
+
+1007
+01:17:05,070 --> 01:17:09,050
+skin puncture و ماباخد منه عين الدم يفضل أني مااخد
+
+1008
+01:17:09,050 --> 01:17:12,370
+منه من هذا المكان Misidentification of the patient
+
+1009
+01:17:13,540 --> 01:17:17,080
+إذا أنا ما اتعرفت على المريض بشكل صحيح معناها راح
+
+1010
+01:17:17,080 --> 01:17:22,120
+يصير في عندى طبعا سلسلة من ال error series of
+
+1011
+01:17:22,120 --> 01:17:26,040
+error ما اتعرفت على ال .. ال .. ال مريض بشكل صحيح
+
+1012
+01:17:26,040 --> 01:17:29,500
+إذا أنا سحبت العين الخطأ من المريض الخطأ و حصلت
+
+1013
+01:17:29,500 --> 01:17:33,780
+على نتائج خطأ للمريض فبالتالي ما ده معناه تشخيص
+
+1014
+01:17:33,780 --> 01:17:38,160
+خاطئ إذا علاج خاطئ إذا هذا سل .. كسلسل من ال error
+
+1015
+01:17:38,160 --> 01:17:43,510
+series of error تمام؟طيب هو ممكن يكون وقعها
+
+1016
+01:17:43,510 --> 01:17:48,490
+disastrous او implication of the patient يعني ..
+
+1017
+01:17:48,490 --> 01:17:54,890
+يعني تأثيرها او وقعها caressي على المريض beta
+
+1018
+01:17:54,890 --> 01:17:59,330
+-diene .. beta-diene او iodine طبعا shouldn't be
+
+1019
+01:17:59,330 --> 01:18:02,110
+used routinely to clean the pleptomy area يعني انا
+
+1020
+01:18:02,110 --> 01:18:11,210
+مش لازم اعقم المكان ب .. ب iodine بحلول iodineطبعا
+
+1021
+01:18:11,210 --> 01:18:15,970
+ليش؟ لإنه طبق ال .. ال contamination ب .. يعني ..
+
+1022
+01:18:15,970 --> 01:18:20,410
+ب .. يعني إنه ييجي beta-diene أو iodine على مكان
+
+1023
+01:18:20,410 --> 01:18:26,190
+ال skin puncture ممكن طبعا يلوث العينة يعني لما
+
+1024
+01:18:26,190 --> 01:18:28,950
+أجي أنا أصحب العينة أصحب العينة و فيها بقايا هذا
+
+1025
+01:18:28,950 --> 01:18:33,380
+ال iodine فبالتالي يسببلي some errortest result
+
+1026
+01:18:33,380 --> 01:18:36,780
+يعني مثلا ارتفاع في ال potassium ارتفاع في ال uric
+
+1027
+01:18:36,780 --> 01:18:39,600
+acid ارتفاع في ال phosphorus result مثلا اجي احكم
+
+1028
+01:18:39,600 --> 01:18:42,700
+على المريض مثلا والله عنده uric acid عالي يعني
+
+1029
+01:18:42,700 --> 01:18:46,260
+مثلا عنده مشكلة في مشاكل في وظائف الكدن و وظائف
+
+1030
+01:18:46,260 --> 01:18:50,240
+الكلة ابدأ اوجه المريض ان هو مثلا ممكن يكون عنده
+
+1031
+01:18:50,240 --> 01:18:54,040
+مشاكل في الكلة او kidney failure او كذامصيبة تمام؟
+
+1032
+01:18:54,040 --> 01:18:58,560
+نتيجة خلط بسيط في جمع العينة ممكن يعني ي .. اوجه
+
+1033
+01:18:58,560 --> 01:19:01,700
+المريض او يعني ان انا يصير في تشخيص خاطئ للمريض
+
+1034
+01:19:01,700 --> 01:19:05,880
+وبالتالي يعني اعرضه لإن هو يتعرض لأدوية او علاجات
+
+1035
+01:19:05,880 --> 01:19:10,040
+ممكن تكون خاطرة عليه skin must be dried لازم انه
+
+1036
+01:19:10,040 --> 01:19:13,220
+انا لما اجي اسحب من العينة .. اسحب عينة من المكان
+
+1037
+01:19:15,150 --> 01:19:18,910
+سواء كان ال .. ال .. الإصبع أو ال heel لازم يكون
+
+1038
+01:19:18,910 --> 01:19:23,110
+جاف تماما، يعني بقايا الكحول اتبخرت أو إن أنا إذا
+
+1039
+01:19:23,110 --> 01:19:26,950
+كان استخدمت .. يعني أنا أقامت بكحول كتير أستخدم ال
+
+1040
+01:19:26,950 --> 01:19:31,750
+.. ال .. جوز، يعني شاش أو قطن معقم حتى أمسح بقايا
+
+1041
+01:19:31,750 --> 01:19:36,110
+هذا الكحولليش؟ لإنه مجرد يا بنات ما أنا أجى أنخذه
+
+1042
+01:19:36,110 --> 01:19:40,130
+يطلع الدم .. يطلع الدم .. التقاء الدم مع الكحول
+
+1043
+01:19:40,130 --> 01:19:44,790
+هيصير في عندي hemolysis of blood، هيتكسر الدم بفعل
+
+1044
+01:19:44,790 --> 01:19:48,460
+التقاء أو ال mixing تبعه مع الكحولهيصير فيه
+
+1045
+01:19:48,460 --> 01:19:54,920
+homolysis يعني تكسر للدم، تمام؟ هذا طبعا خطأ يعني،
+
+1046
+01:19:54,920 --> 01:19:58,780
+فإذا أنا حصلت على دم متكسر، خلايا الدم متكسرت
+
+1047
+01:19:58,780 --> 01:20:01,520
+فبالتالي أنا مش راح أقدر أعدها، في حصات كتير مش
+
+1048
+01:20:01,520 --> 01:20:06,490
+راح أقدر أجريها على ال homolized blood، تمام؟طيب
+
+1049
+01:20:06,490 --> 01:20:12,330
+لو أنا خلّيت .. طبعا استخدمت كحولي كتير و خلّيته
+
+1050
+01:20:12,330 --> 01:20:15,670
+فترة .. استنيت عليه فترة لما يجف .. إيش معا .. إيش
+
+1051
+01:20:15,670 --> 01:20:21,750
+رح يصير؟ طبعا يا بنات الكحول لما بيتبخر و بيتطير
+
+1052
+01:20:21,750 --> 01:20:26,470
+بيصير معاه .. يعني بيسحب معاه سائل، ماء، بيسحب
+
+1053
+01:20:26,470 --> 01:20:29,730
+معاه ماء، يعني انت ..حاليا حط الكحول على إيديك و
+
+1054
+01:20:29,730 --> 01:20:33,770
+السبيل يتبخر، بتحسي ببرودة، البرودة هذه ناتجي
+
+1055
+01:20:33,770 --> 01:20:36,710
+نتيجة تبخر الماء اللي موجود في خلايانا أو بين
+
+1056
+01:20:36,710 --> 01:20:41,470
+الخلايا و بعديها بتحسي بجفاف للبشرة، تمام؟ طيب،
+
+1057
+01:20:41,470 --> 01:20:46,210
+إذا أنا حطيت كحول كتير على مكان السحب و إستنيت
+
+1058
+01:20:46,210 --> 01:20:50,930
+لبين ميط طايرمعناها هيتبخر الماء من الموجود ما بين
+
+1059
+01:20:50,930 --> 01:20:54,990
+الخلايا أو الموجود في .. حتى ممكن يكون موجود في ال
+
+1060
+01:20:54,990 --> 01:20:57,670
+capillary في الأوعية الدموية الضيقة أو الصغيرة
+
+1061
+01:20:57,670 --> 01:21:01,870
+اللي موجودة في المكان فبالتالي راح يتركز الدم راح
+
+1062
+01:21:01,870 --> 01:21:04,390
+يصير في عندي حاجة اسمها hemoconcentration طالما
+
+1063
+01:21:04,390 --> 01:21:10,310
+أنه صار في عندي فقدلأس .. ل .. ل .. للماء و الدم
+
+1064
+01:21:10,310 --> 01:21:14,850
+أصلا مكوناته ماء مش احنا حكينا لبلازمة 90% منها
+
+1065
+01:21:14,850 --> 01:21:19,050
+ماء تمام و الخلايا معلقة فيها لذا المدام صار فيه
+
+1066
+01:21:19,050 --> 01:21:22,970
+تبخر معناه فقدار للماء لذا التركيز للدم لذا في
+
+1067
+01:21:22,970 --> 01:21:25,770
+عندي حاجة اسمها hemoconcentration إذا بدي أفحص
+
+1068
+01:21:25,770 --> 01:21:28,930
+مكونات الدم كلشي هلاجيه عالي ال vitaminات ال
+
+1069
+01:21:28,930 --> 01:21:32,230
+enzymes ال proteinات ال .. ال waste ال hormoneات
+
+1070
+01:21:32,230 --> 01:21:35,640
+كلشي عاليفبالتالي هنا بيكون بقى أنا ببدأ أحكم على
+
+1071
+01:21:35,640 --> 01:21:38,540
+المريض أن هو عنده مشاكل و هو لأ يعني المشكلة مني
+
+1072
+01:21:38,540 --> 01:21:43,900
+أنا أثناء السحب فلازم أنتبه لهذه النقطة و يعني
+
+1073
+01:21:43,900 --> 01:21:47,900
+خطوات و تعليمات اللي احنا بنحكيها يعني السحب يا
+
+1074
+01:21:47,900 --> 01:21:54,800
+بنات عبارة عن يعني لحظي في مجرد يعني دقيقة دقيقتين
+
+1075
+01:21:54,800 --> 01:21:59,770
+بتكون انت خلاص يعني خلصتي عملية السحبلكن التعليمات
+
+1076
+01:21:59,770 --> 01:22:04,470
+اللي أثناء السحب و ما قبل السحب و ما بعد السحب هي
+
+1077
+01:22:04,470 --> 01:22:08,190
+اللي مهمة عشان أنا أحصل على ال proper sample،
+
+1078
+01:22:08,190 --> 01:22:15,210
+العينة المثلة، تمام؟ طيبرح أتوقف لهنا بالتسجيل
+
+1079
+01:22:15,210 --> 01:22:18,330
+لعند ال van puncture و ان شاء الله رح أسجل ال van
+
+1080
+01:22:18,330 --> 01:22:23,850
+puncture بتسجيل جديد يعني سحب العينة من وريد علشان
+
+1081
+01:22:23,850 --> 01:22:29,770
+يكون الحجم التسجيل مناسب و أيضا يعني بإمكانكم انكم
+
+1082
+01:22:29,770 --> 01:22:32,470
+انتوا تتعاملوا معاه يعني انا مايكونش في عندنا
+
+1083
+01:22:32,470 --> 01:22:35,110
+مشاكل في رفعه على ال drive او على يوتيوب او
+
+1084
+01:22:35,110 --> 01:22:39,120
+whateverيعطيكم العافية، اقراكم ان شاء الله في ال
+
+1085
+01:22:39,120 --> 01:22:44,460
+part two of blood collection اللي بيتحدث عن ال van
+
+1086
+01:22:44,460 --> 01:22:44,980
+puncture
+
diff --git a/PL9fwy3NUQKwY9jl8xw-bWkVZkWcGGyspd/6stpniKSY70.srt b/PL9fwy3NUQKwY9jl8xw-bWkVZkWcGGyspd/6stpniKSY70.srt
new file mode 100644
index 0000000000000000000000000000000000000000..17d39dd9262aa65d1c002adec6968f701a7f1e00
--- /dev/null
+++ b/PL9fwy3NUQKwY9jl8xw-bWkVZkWcGGyspd/6stpniKSY70.srt
@@ -0,0 +1,2336 @@
+1
+00:00:04,390 --> 00:00:07,410
+السلام عليكم ورحمة الله وبركاته عزيزات الطالبات
+
+2
+00:00:07,410 --> 00:00:13,150
+معكم إن شاء الله في المحاضرة نظرية رقم اثنين لمساق
+
+3
+00:00:13,150 --> 00:00:17,830
+مقدمة في التحاليل الطبية إن شاء الله اليوم راح نأخذ
+
+4
+00:00:17,830 --> 00:00:22,790
+ناخذ مختصراً أو نبدأ عن الـ solutions المحاليل التي
+
+5
+00:00:22,790 --> 00:00:26,810
+تستخدم في المختبرات الطبية solutions used in
+
+6
+00:00:26,810 --> 00:00:30,590
+medical laboratories المحاليل التي تستخدم في
+
+7
+00:00:30,590 --> 00:00:36,270
+المختبرات الطبية. بداية، بدنا نعرف إيش يعني solution
+
+8
+00:00:36,270 --> 00:00:40,090
+solution كمحلول هو عبارة عن
+
+9
+00:00:40,090 --> 00:00:43,950
+homogeneous mixture هو عبارة عن خليط متجانس من
+
+10
+00:00:43,950 --> 00:00:47,550
+مكونين أو أكثر. طبعاً، إذا هو عبارة عن homogeneous
+
+11
+00:00:47,550 --> 00:00:52,910
+mixture of two or more substances. طبعاً، طيب كمحلول
+
+12
+00:00:52,910 --> 00:00:58,590
+لا بد أن يحتوي أو يتكون من شغلتين: solute التي هي
+
+13
+00:00:58,590 --> 00:01:03,970
+الأشياء التي تم إذابتها في المحلول، و الـ solvent
+
+14
+00:01:03,970 --> 00:01:09,390
+طبعاً، فالـ solute هو عبارة عن الـ dissolved substance،
+
+15
+00:01:09,390 --> 00:01:13,870
+المادة المذابة، whereas the solvent is a substance
+
+16
+00:01:13,870 --> 00:01:17,230
+in which the solute is dissolved to make the mixture. إذا
+
+17
+00:01:17,230 --> 00:01:21,970
+المحلول هو عبارة عن خليط من مكونات أو أكثر،
+
+18
+00:01:21,970 --> 00:01:26,690
+والمكونات هذه ممكن تنقسم إلى: إما أن تكون solute، يعني
+
+19
+00:01:26,690 --> 00:01:32,510
+أشياء تم إذابتها، تمام، أشياء مذابة، و solvent المذيب
+
+20
+00:01:32,510 --> 00:01:39,720
+هو المادة التي الـ solute تذوب فيه، تمام. ففي عندي
+
+21
+00:01:39,720 --> 00:01:43,560
+أنواع... يعني أنواع مختلفة من المحاليل التي تستخدم
+
+22
+00:01:43,560 --> 00:01:47,060
+في الـ medical laboratories و التجارب التي نجريها في
+
+23
+00:01:47,060 --> 00:01:52,180
+داخل المختبر الطبي. من خلال أو من ضمن هذه المحاليل،
+
+24
+00:01:52,180 --> 00:01:56,480
+يعني ممكن نحن نصنفها إلى: reagent solution نوع من
+
+25
+00:01:56,480 --> 00:02:00,480
+أنواع المحاليل التي أسميها reagent solution. نوع آخر
+
+26
+00:02:00,480 --> 00:02:04,900
+أسميه staining solution، و standard solution، and
+
+27
+00:02:04,900 --> 00:02:08,420
+buffer solution. راح نأخذ كل واحدة على حدة و نعرف
+
+28
+00:02:08,420 --> 00:02:11,240
+إيش يعني reagent، إيش يعني staining،
+
+29
+00:02:11,240 --> 00:02:15,500
+solution، standard، and buffer solution. خلينا نبدأ
+
+30
+00:02:15,500 --> 00:02:18,980
+بأول واحدة، التي هي reagent solutions. إيش يعني reagent
+
+31
+00:02:18,980 --> 00:02:23,140
+solution؟ طبعاً نحن نعرف أنه، بس نجري فحصاً
+
+32
+00:02:23,140 --> 00:02:27,360
+طبيعياً على عينة مريض، لا بد أنه، مثلاً، على فرض أنني أريد
+
+33
+00:02:27,360 --> 00:02:30,960
+أقيس مستوى السكر في الدم، طبعاً أنا أريد أقيس الـ
+
+34
+00:02:30,960 --> 00:02:34,420
+glucose، مستوى السكر في الدم، الذي هو الـ glucose. طيب،
+
+35
+00:02:35,150 --> 00:02:39,090
+لا بد من أن يكون في عندي مادة متفاعلة تتفاعل مع الـ
+
+36
+00:02:39,090 --> 00:02:44,170
+glucose في الدم، لكي في النهاية تعطيني إما لوناً أو
+
+37
+00:02:44,170 --> 00:02:49,330
+تفاعلاً، يعني سيحدث change، سيحدث تغير معين أنا
+
+38
+00:02:49,330 --> 00:02:53,770
+أقدر يعني أقيس هذا التغير، وبالتالي هذا التغير
+
+39
+00:02:53,770 --> 00:02:58,210
+يعبّر لي عن تركيز الـ glucose في الدم. نرجع ثانيةً نعيد
+
+40
+00:02:58,210 --> 00:03:02,790
+نحكي أنه، على فرض أنني أريد أقيس مستوى السكر في الدم،
+
+41
+00:03:02,790 --> 00:03:07,450
+لا بد من وجود مادة متفاعلة تتفاعل مع السكر هذا، مع
+
+42
+00:03:07,450 --> 00:03:12,110
+الـ glucose، و تعطيني تغييراً معيناً. التغير هذا ممكن
+
+43
+00:03:12,110 --> 00:03:16,370
+يكون تغييراً في اللون، ممكن يكون تصاعد غازات، ممكن يكون
+
+44
+00:03:16,510 --> 00:03:21,410
+ممكن يكون ظهور فقاعات، ممكن يكون يعني أي نوع من
+
+45
+00:03:21,410 --> 00:03:24,370
+التغير، حتى يعني رائحة، بس نحن لا نعتمد كثيراً على
+
+46
+00:03:24,370 --> 00:03:28,650
+الروائح والأشياء هذه. نحن دائماً التغيرات تكون
+
+47
+00:03:28,650 --> 00:03:33,990
+تغييراً في اللون، أو حتى ظهور غاز أو تصاعد غازات أو
+
+48
+00:03:33,990 --> 00:03:39,650
+فقاعات، يعني أشياء يمكن قياسها، التغير يمكن قياسه،
+
+49
+00:03:39,650 --> 00:03:43,950
+تمام. بمجرد تفاعل هذا الـ reagent أو المادة
+
+50
+00:03:43,950 --> 00:03:49,790
+المتفاعلة مع الـ glucose، حدوث تغير، لا بد أن يكون
+
+51
+00:03:49,790 --> 00:03:54,490
+التغير هذا يمكن قياسه، بحيث أنه يعبّر لي عن مستوى
+
+52
+00:03:54,490 --> 00:03:58,630
+السكر في الدم. يعني لو فرضنا أن المادة المتفاعلة
+
+53
+00:03:58,630 --> 00:04:01,590
+التي ستتفاعل مع هذا الـ glucose تعطيني لوناً pink،
+
+54
+00:04:02,140 --> 00:04:07,500
+طبعاً لون pink. فخلينا نفرض مثلاً، كلما زادت شدة
+
+55
+00:04:07,500 --> 00:04:12,900
+اللون، كلما زاد الـ pink، طبعاً سيُعطيني... هي... هيُخيّل
+
+56
+00:04:12,900 --> 00:04:18,880
+في ذهني أنه في عندي كمية كبيرة من التفاعل صارت،
+
+57
+00:04:18,880 --> 00:04:23,480
+يعني كمية كبيرة من الـ glucose تفاعلت مع الـ reagent
+
+58
+00:04:23,480 --> 00:04:29,550
+و أعطتني اللون الـ pink. فبالتالي، إذا التغير في اللون
+
+59
+00:04:29,550 --> 00:04:33,870
+هذا الذي أنا قدرت أقيصه، قدرت أعرف إيش شدة اللون،
+
+60
+00:04:33,870 --> 00:04:39,790
+تمام، يُعبّر لي عن قَدْر ما في عندي تركيز في العينة. تمام. هذا
+
+61
+00:04:39,790 --> 00:04:43,110
+كتقديم للـ reagent solution. خلينا نشوف إيش مكتوب
+
+62
+00:04:43,110 --> 00:04:47,630
+عندنا بالـ reagent solution. هو عبارة عن أي محلول
+
+63
+00:04:47,630 --> 00:04:52,470
+يمكن استخدامه that is used in conjunction with a
+
+64
+00:04:52,470 --> 00:04:57,770
+given sample. يمكن أن يتم استخدامه مع عينة، مع العينة،
+
+65
+00:04:57,770 --> 00:05:02,490
+تمام، و بالتالي expected to produce a measurable or
+
+66
+00:05:02,490 --> 00:05:06,630
+noticeable change. سيحدث بمجرد ما يتفاعل مع مكوّن من
+
+67
+00:05:06,630 --> 00:05:11,210
+مكونات العينة. هذا الـ reagent سيحدث measurable or
+
+68
+00:05:11,210 --> 00:05:16,350
+noticeable change. يعني سيحدث تغير يمكن ملاحظته أو
+
+69
+00:05:16,350 --> 00:05:21,810
+يمكن قياسه. فهذا المحلول الذي يُحقّق الشرط هذا أنه
+
+70
+00:05:21,810 --> 00:05:26,370
+يتفاعل مع مكوّن من مكونات العينة، ويحدث تغييراً،
+
+71
+00:05:26,370 --> 00:05:30,770
+التغير هذا سيحدث تغير على العينة، يمكن قياسه أو
+
+72
+00:05:30,770 --> 00:05:35,610
+ملاحظته على طول، أُسمّي هذا المحلول عبارة عن reagent
+
+73
+00:05:35,610 --> 00:05:40,050
+solution، تمام؟ طيب، وأنا أشتغل، طبعاً الـ reagent
+
+74
+00:05:40,050 --> 00:05:44,370
+solution، هذه المحاليل المتفاعلة، لما أ... أنا أطلبها
+
+75
+00:05:44,370 --> 00:05:49,490
+من الشركات، طبعاً كل نوع فحص له reagent خاص به،
+
+76
+00:05:49,490 --> 00:05:52,910
+يعني لما أريد أقيس مستوى الدهون في الجسم، ب... ب...
+
+77
+00:05:52,910 --> 00:05:56,150
+أستخدم reagent خاصاً في الكشف عن الدهون، لما أريد
+
+78
+00:05:56,150 --> 00:06:00,750
+أقيس مستوى السكر في الدم، أستخدم reagent متخصصاً في
+
+79
+00:06:00,750 --> 00:06:05,060
+الكشف عن الـ glucose. لو أريد أقيس مستوى بروتينات، أريد
+
+80
+00:06:05,060 --> 00:06:08,900
+أستخدم reagent متخصصاً في الكشف عن البروتينات. نشاط
+
+81
+00:06:08,900 --> 00:06:12,320
+إنزيمات، أستخدم reagent متخصصاً في الكشف عن نشاط
+
+82
+00:06:12,320 --> 00:06:16,860
+الإنزيمات، وهكذا. إذا هذه الـ reagents أنا أشتريها
+
+83
+00:06:16,860 --> 00:06:21,340
+من شركات خاصة، هي تصمّم لي الفحص، يعني هي تصمّم هذا
+
+84
+00:06:21,340 --> 00:06:25,720
+الـ reagent، و ت... تعرف تركيبه الكيميائي، و تصمّمه
+
+85
+00:06:25,720 --> 00:06:30,280
+على أساس أنّه يكون متخصصاً لإجراء فحص طبي معيّن. طيب،
+
+86
+00:06:30,670 --> 00:06:34,830
+هذا الـ reagent، لما أنا أشتريه من الشركة، ممكن يكون
+
+87
+00:06:34,830 --> 00:06:40,050
+جاهزاً، ready to use، تمام، ممكن يكون جاهزاً، ready to use،
+
+88
+00:06:40,050 --> 00:06:44,570
+أو ممكن يكون عبارة عن powder، وأنا أريد أُحلّه، أريد
+
+89
+00:06:44,570 --> 00:06:47,830
+أُحوّله إلى محلول، يعني أضيف عليه solvent. الـ solvent
+
+90
+00:06:47,830 --> 00:06:51,430
+هذا ممكن تكون ماء، distilled water، تمام، أو أي محلول
+
+91
+00:06:51,430 --> 00:06:56,570
+تُوصي به الشركة. فإذا بيجيني powders، solute يكون
+
+92
+00:06:56,570 --> 00:07:00,370
+solute، تمام، وأنا أضيف عليه solvent لكي أُكوّن
+
+93
+00:07:00,370 --> 00:07:06,130
+solution، تمام. أو ممكن يجيني مركزاً، وأنا أُخفّفه بحيث
+
+94
+00:07:06,130 --> 00:07:10,590
+أحصل على التخفيف المناسب للتجربة تبعي أو للفحص
+
+95
+00:07:10,590 --> 00:07:17,730
+تبعي. تمام. أصبح في عندي necessary care، اهتمام، و
+
+96
+00:07:17,730 --> 00:07:24,610
+انتباه أثناء تحضير هذا الـ reagent. لازم يكون في عندي
+
+97
+00:07:24,610 --> 00:07:28,150
+care، اهتمام، including the following should be
+
+98
+00:07:28,150 --> 00:07:31,750
+taken in preparing a given reagent solution. وأنا
+
+99
+00:07:31,750 --> 00:07:35,830
+أُحضّر reagent solution، لازم يكون في عندي انتباه
+
+100
+00:07:35,830 --> 00:07:40,770
+يستدعي أنني أحافظ على الأشياء التالية. إيش هي
+
+101
+00:07:40,770 --> 00:07:43,110
+إيش الذي أحتاج أن أحافظ عليه؟ أول حاجة، الـ chemical
+
+102
+00:07:43,110 --> 00:07:47,740
+selection. إيش يعني chemical selection؟ يعني أنا أختار
+
+103
+00:07:47,740 --> 00:07:51,400
+الـ reagent الكيميائي المناسب لفحصي. إذا أنا أريد
+
+104
+00:07:51,400 --> 00:07:54,960
+أقيس بروتينات، لا يُنفع أستخدم reagent يكشف عن وجود
+
+105
+00:07:54,960 --> 00:07:59,180
+مثلاً السكر في الدم. لازم يكون إيش؟ الـ reagent مختصّ
+
+106
+00:07:59,180 --> 00:08:02,900
+بهذا الفحص تبعي، ونحن سبق وحكينا أن كل reagent
+
+107
+00:08:02,900 --> 00:08:07,540
+يكون مصمّماً لإجراء فحص معيّن. فأنا لازم أكون عندي الـ
+
+108
+00:08:07,540 --> 00:08:12,390
+selection تبع الاختيار لهذا الـ reagent. اختيار المادة
+
+109
+00:08:12,390 --> 00:08:15,790
+الكيميائية المناسبة لهذا الـ reagent بحيث أنّه
+
+110
+00:08:15,790 --> 00:08:19,670
+يتناسب مع الفحص الطبيعي، تمام؟ وإذا لم يُنفع، أنا يكون
+
+111
+00:08:19,670 --> 00:08:23,950
+عندي فحص أريد أُقيس مثلاً مستوى الدهون في الجسم، و
+
+112
+00:08:23,950 --> 00:08:28,430
+أذهب أختار، أُجري selection لـ reagent، المادة
+
+113
+00:08:28,430 --> 00:08:31,190
+الكيميائية التي فيه متخصصة في الكشف عن البروتينات،
+
+114
+00:08:31,190 --> 00:08:35,750
+وليس الدهون، خطأ. ساعتها الفحص الطبيعي سيكون خاطئاً، أنا
+
+115
+00:08:35,750 --> 00:08:40,930
+لن آخذ نتيجة على مستوى الدهون، سآخذ نتيجة على
+
+116
+00:08:40,930 --> 00:08:46,190
+مستوى البروتينات، وهذا خطأ. طيب، الشغل الثاني الذي
+
+117
+00:08:46,190 --> 00:08:49,290
+أحتاج أن أُنتبه له: following instructions of preparation،
+
+118
+00:08:49,290 --> 00:08:54,290
+تذكرون لما حكينا في محاضرة الـ safety، أن كل مادة
+
+119
+00:08:54,290 --> 00:09:01,950
+كيميائية يكون عليها label، تكون معلّمة، label عليها
+
+120
+00:09:04,780 --> 00:09:08,880
+بالاسم، والتركيبة الكيميائية، و الـ expiration date،
+
+121
+00:09:08,880 --> 00:09:13,940
+الذي هو تاريخ الانتهاء، صلاحية، و... يعني خطوات
+
+122
+00:09:13,940 --> 00:09:18,040
+أثناء تحضير هذا المحلول، أو يعني تعليمات لتحضيره،
+
+123
+00:09:18,040 --> 00:09:22,020
+تعليمات لاستخدامه، تعليمات للمحافظة على سلامتنا
+
+124
+00:09:22,020 --> 00:09:26,160
+أثناء استخدامه. ممكن يكون عليه علامات hazard،
+
+125
+00:09:26,160 --> 00:09:29,940
+symbols، إشارات على هذا الـ reagent أو هذه المادة
+
+126
+00:09:29,940 --> 00:09:32,680
+الكيميائية بحيث أنها تُنَبّهني: هذا حامض، هذا
+
+127
+00:09:32,680 --> 00:09:39,500
+حارق، هذا ممكن يُخرج منه غازات ويُسبب اختناقاً،
+
+128
+00:09:39,500 --> 00:09:43,560
+هذا ممكن ي...يخرج منه إشعاعات. فهذه كلها تكون
+
+129
+00:09:43,560 --> 00:09:47,480
+موجودة أين؟ على الـ label. فأنا لازم أقرأ الـ label
+
+130
+00:09:47,480 --> 00:09:55,340
+كثيراً جيّداً، وأنتبه، وأتبع تعليمات التحضير جيّداً. لماذا؟
+
+131
+00:09:55,340 --> 00:10:00,020
+لأنني أريد أن أُحضّره بجودة عالية لكي أحصل على
+
+132
+00:10:00,020 --> 00:10:06,060
+reagent تركيزه دقيق. فبالتالي، يُفحص لي أو يُشغّل لي الفحص
+
+133
+00:10:06,060 --> 00:10:11,400
+بشكل دقيق، لكي أحصل على نتائج دقيقة، وبالتالي أساهم
+
+134
+00:10:11,400 --> 00:10:16,580
+في الـ accurate diagnosis، و ال... الـ valuable، إيش
+
+135
+00:10:16,580 --> 00:10:23,020
+treatment، في التشخيص الدقيق والعلاج الفعال، تمام؟
+
+136
+00:10:24,090 --> 00:10:28,050
+طيب، إذا الخطوة الثالثة أو الشغل الثالث الذي أنا
+
+137
+00:10:28,050 --> 00:10:31,130
+أحتاج أن أُنتبه له أثناء تحضير الـ reagent، أنه أنا أريد
+
+138
+00:10:31,130 --> 00:10:36,170
+أُستخدم using accurate measurement of substances،
+
+139
+00:10:36,170 --> 00:10:41,330
+تمام؟ حكينا أنه لازم أكون منتبهة كثيراً على
+
+140
+00:10:41,330 --> 00:10:46,770
+الحجوم. قَدْ يَطْلُب مثلاً... يحكي لي مثلاً خذي من
+
+141
+00:10:46,770 --> 00:10:54,010
+هذه المادة خمسة سنتيمترات، واخلطيهم بلتر ماء، تمام؟ لا يُنفع
+
+142
+00:10:54,010 --> 00:10:59,620
+أنني أخلطهم بلتر وربع، أو لتر ونصف، خطأ. لازم
+
+143
+00:10:59,620 --> 00:11:02,980
+أنني أُجري accurate measurement of substances، لازم
+
+144
+00:11:02,980 --> 00:11:07,800
+أن يكون عندي، أُحضّره بأحجام دقيقة لكي أحصل على
+
+145
+00:11:07,800 --> 00:11:12,340
+تركيز دقيق، وبالتالي الشغل يكون كمان دقيقاً. using of
+
+146
+00:11:12,340 --> 00:11:15,700
+appropriate type of glassware or plasticware. إذا هذا
+
+147
+00:11:15,700 --> 00:11:20,420
+المحلول أنا أريد أُحضّره، بعد ذلك أحتاج أن أضعه في bottle، لازم
+
+148
+00:11:20,420 --> 00:11:23,980
+الـ bottle تكون مناسبة للحفاظ على الـ reagent، كذا
+
+149
+00:11:23,980 --> 00:11:28,680
+يعني، في reagents، وسيَمُرّ معكم خلال رحلتكم في دراسة
+
+150
+00:11:28,680 --> 00:11:33,880
+التحاليل الطبية، سيَمُرّ معكم أنواع من الـ reagents،
+
+151
+00:11:33,880 --> 00:11:40,580
+يُفضّل حفظها في dark bottle، يعني هي حساسة للضوء، و
+
+152
+00:11:40,580 --> 00:11:45,080
+لازم أنا أُضعها في bottle لونها بني أو أسود. سيَمُرّ
+
+153
+00:11:45,080 --> 00:11:50,970
+معنا مثلاً الـ acid، و... والـ acid، وال... والـ base،
+
+154
+00:11:50,970 --> 00:11:55,970
+لازم أضعهم في glass container، بس لازم الأغطية
+
+155
+00:11:55,970 --> 00:11:59,350
+تبعهم لا تكون glass. أخذنا هذه المعلومة في المحاضرة
+
+156
+00:11:59,350 --> 00:12:02,190
+الماضية. حكينا أنه ليس من اللازم أن تكون الـ container...
+
+157
+00:12:02,190 --> 00:12:07,990
+الأغطية glass، لكي لا ت... يعني تلتصق ويصعب فتحها،
+
+158
+00:12:08,540 --> 00:12:13,100
+تمام؟ في بعض المحاليل لازم أضعها في plastic bottle،
+
+159
+00:12:13,100 --> 00:12:17,540
+حسب. فبالتالي نوع الـ reagent هو الذي يُحكِم لي، أو
+
+160
+00:12:17,540 --> 00:12:20,800
+تركيبه الكيميائي هو الذي يُحكِم لي الـ better أو الـ
+
+161
+00:12:20,800 --> 00:12:26,780
+best container له. طبعاً هذا بالنسبة لي الـ reagent
+
+162
+00:12:26,780 --> 00:12:29,040
+solution. إذا ما أريد أن أعرفه عن الـ reagent
+
+163
+00:12:29,040 --> 00:12:33,120
+solution، أنه هو عبارة عن المادة المتفاعلة مع العينة،
+
+164
+00:12:33,120 --> 00:12:36,820
+التي من شأنها أن تعطيني change على العينة، تغييراً على
+
+165
+00:12:36,820 --> 00:12:42,860
+العينة، يمكن قياسه ويمكن ملاحظته، بحيث أنني بعد
+
+166
+00:12:42,860 --> 00:12:48,640
+ذلك أحصل على قراءات أو أحصل على نتيجة. طبعاً ولا بدّ أن
+
+167
+00:12:48,640 --> 00:12:54,470
+أنا أُستدعي الانتباه والاهتمام أثناء اختيار نوع الـ
+
+168
+00:12:54,470 --> 00:12:59,210
+reagent، يكون ملائماً لنوع الفحص الذي نحن بصدد
+
+169
+00:12:59,210 --> 00:13:04,550
+إجرائه، وأيضاً أريد أن أتبع التعليمات أثناء تحضيره،
+
+170
+00:13:04,550 --> 00:13:08,010
+تعليمات الشركة المصنعة لهذا الـ reagent، أريد
+
+171
+00:13:08,010
+
+223
+00:17:02,840 --> 00:17:07,700
+خلايا حتى تمام؟ إذا النوع الآخر هو الـ staining
+
+224
+00:17:07,700 --> 00:17:10,580
+solution تمام؟ إيش بدي أعرف عن الـ staining
+
+225
+00:17:10,580 --> 00:17:14,100
+solution؟ أول حاجة بدي أعرف إن هو مكون من colored
+
+226
+00:17:14,100 --> 00:17:18,990
+dye من صبغات ملونة تمام؟ ممكن يكون شحنته موجبة يعني
+
+227
+00:17:18,990 --> 00:17:23,470
+تركيبها basic أو تكون شحنته لهذا المحلول يعني الـ
+
+228
+00:17:23,470 --> 00:17:27,250
+staining solution تكون شحنته سالبة تمام يعني هو
+
+229
+00:17:27,250 --> 00:17:30,730
+تركيبها acidic أو تكون شحنته صفر يعني هو neutral
+
+230
+00:17:30,730 --> 00:17:34,710
+متعادل لا هو basic ولا acidic طب أنا إيش بستفيد من
+
+231
+00:17:34,710 --> 00:17:39,390
+أن أنا أعرف شحنة الـ solution بستخدمها الاستخدام
+
+232
+00:17:39,390 --> 00:17:43,230
+الصحيح يعني أنا لو بدي أصبغ نواة الخلية نواة
+
+233
+00:17:43,230 --> 00:17:48,930
+الخلية مليانة أحماض نووية ما لها نقاش أحماض نووية
+
+234
+00:17:48,930 --> 00:17:56,550
+DNA و RNA فطبعا بما أنها هي فيها أحماض نووية لازم
+
+235
+00:17:56,550 --> 00:18:00,430
+أن أنا أصبغها بالـ basic solution اللي هي المحاليل
+
+236
+00:18:00,430 --> 00:18:05,110
+القاعدية طبعا هي بده تكون شحنتها سالبة لأنها أحماض
+
+237
+00:18:05,110 --> 00:18:11,570
+تمام، والصبغة هتكون شحنتها موجبة لأنها basic فموجب
+
+238
+00:18:11,570 --> 00:18:15,830
+مع سالب يتم التجاذب والالتقاء وبالتالي الصبغة
+
+239
+00:18:15,830 --> 00:18:21,210
+راح ترتبط بالجزء اللي مراد صباغته وبالتالي ممكن
+
+240
+00:18:21,210 --> 00:18:25,490
+بتمكنني من رؤيته تحت الـ microscope فبالتالي هذا
+
+241
+00:18:25,490 --> 00:18:29,370
+اللي بستفيده من معرفة الشحنة وحكينا إنه أنا في
+
+242
+00:18:29,370 --> 00:18:32,990
+عندي أنواع كثيرة من الصبغات اللي تستخدم في الـ
+
+243
+00:18:32,990 --> 00:18:36,430
+medical laboratories اللي بتعطي المواد المراد
+
+244
+00:18:36,430 --> 00:18:39,210
+رؤيتها أو الأجزاء المراد رؤيتها تحت الـ microscope
+
+245
+00:18:39,210 --> 00:18:43,370
+بتعطيها artificial color لون اصطناعي طبعا أنا ممكن
+
+246
+00:18:43,370 --> 00:18:49,050
+أصبغ عينات الدم سواء الـ urine الخلايا الـ كل
+
+247
+00:18:49,050 --> 00:18:55,490
+شيء يعني مسحات البكتيريا أي نوع يعني عينة clinical
+
+248
+00:18:55,490 --> 00:18:59,310
+sample يعني عينة تم أخذها من مريض ممكن أصبغها
+
+249
+00:18:59,310 --> 00:19:03,930
+لرؤيتها أو يعني لفحصها تحت الـ microscope طيب
+
+250
+00:19:03,930 --> 00:19:08,810
+نوع ثالث من المحاليل هو عبارة عن الـ standard
+
+251
+00:19:08,810 --> 00:19:12,710
+solutions إيش يعني standard solution؟ طبعا
+
+252
+00:19:12,710 --> 00:19:17,130
+standard solution يعني محاليل معيارية إيش يعني
+
+253
+00:19:17,130 --> 00:19:21,210
+معيارية؟ يعني أنا بعاير ب ... ب ... ب ... يعني
+
+254
+00:19:21,210 --> 00:19:26,430
+باستخدام هذا المحلول كيف بعاير يعني؟ يعني مثلا أنا
+
+255
+00:19:26,430 --> 00:19:34,570
+العينة تبعيتي هي فيها تركيز مجهول تمام؟ والـ
+
+256
+00:19:34,570 --> 00:19:41,090
+standard solution بيكون تركيزه معلوم تمام؟ طيب فالـ
+
+257
+00:19:41,090 --> 00:19:46,790
+... التركيز هذه المعلومة أنا بستخدمها يعني علشان
+
+258
+00:19:46,790 --> 00:19:52,260
+أعرف التركيز داخل العينة يعني أنا بستخدم محاليل
+
+259
+00:19:52,260 --> 00:19:58,140
+معلومة التركيز علشان أحصل على علاقة التركيز المجهول
+
+260
+00:19:58,140 --> 00:20:02,260
+داخل العينة إذا أنا بجيب التركيز داخل العينة
+
+261
+00:20:02,260 --> 00:20:07,420
+بدلالة معلوم اللي هو الـ standard solution طيب كيف
+
+262
+00:20:07,420 --> 00:20:11,600
+بيصير هذا الـ issue لنفرض مثلا خليني ... لنفرض أن
+
+263
+00:20:11,600 --> 00:20:18,660
+عندي محلول standard solution تمام وعينة مريض لو أنا
+
+264
+00:20:18,660 --> 00:20:22,880
+أضفت عليهم الـ reagent المادة المتفاعلة مثلا عشان
+
+265
+00:20:22,880 --> 00:20:29,120
+تتفاعل مع الـ glucose وتعطني لون، تمام؟ طيب فعليا
+
+266
+00:20:29,120 --> 00:20:33,780
+... فعليا الـ standard solution أنا عارفة تركيزه هو
+
+267
+00:20:33,780 --> 00:20:36,980
+دائما بيكون معلوم التركيز لو نفرض أنه تركيزه ...
+
+268
+00:20:36,980 --> 00:20:43,140
+تركيز السكر داخل المحلول هذا مئة تمام وأنا أضفت
+
+269
+00:20:43,140 --> 00:20:48,100
+لهم reagent وأعطاني لون pink نفرض فلو أعطى في
+
+270
+00:20:48,100 --> 00:20:54,220
+العينة نصف شدة اللون تمام نصف شدة اللون اللي موجودة
+
+271
+00:20:54,220 --> 00:20:58,460
+في الـ standard راح أعرف أن تركيز العينة تبعيتي
+
+272
+00:20:58,460 --> 00:21:05,030
+خمسين طبعا لأن المعلوم مئة لو أعطت العينة ضعف شدة
+
+273
+00:21:05,030 --> 00:21:11,970
+اللون عن الـ standard رح أعرف إنه في العينة تبعيتي
+
+274
+00:21:11,970 --> 00:21:18,570
+يعني تركيز مئتين ضعف طبعا إذا أنا في عندي جهاز
+
+275
+00:21:18,570 --> 00:21:22,350
+بيقيس شدة اللون اللي بيظهر من التفاعل وبيقارن
+
+276
+00:21:22,350 --> 00:21:28,320
+لها باستخدام الـ standard solution وبالتالي بيقارن
+
+277
+00:21:28,320 --> 00:21:31,720
+لها باستخدام standard solution وبالتالي بيحسب لي
+
+278
+00:21:31,720 --> 00:21:35,960
+التركيز المجهول داخل العينة بدلالة معلوم تمام؟
+
+279
+00:21:35,960 --> 00:21:39,880
+طيب they are used to determine the value of an
+
+280
+00:21:39,880 --> 00:21:42,760
+identical chemical with unknown concentration of a
+
+281
+00:21:42,760 --> 00:21:46,200
+given solution يعني احنا حكينا أن هذه المحاليل
+
+282
+00:21:46,200 --> 00:21:51,180
+تستخدم حتى نعرف إيش to determine the value of
+
+283
+00:21:51,180 --> 00:21:54,060
+an identical chemical with an unknown concentration
+
+284
+00:21:54,060 --> 00:21:59,420
+يعني أنا بدي أعرف كمية تركيز مادة معينة مجهولة في
+
+285
+00:21:59,420 --> 00:22:03,500
+المحلول أو في العينة تبعيتي طيب ال ... ال ... ال
+
+286
+00:22:03,500 --> 00:22:06,580
+chemicals that are used to prepare these solutions
+
+287
+00:22:06,580 --> 00:22:10,280
+should be of analytical grade لازم المحاليل
+
+288
+00:22:10,280 --> 00:22:13,660
+المستخدمة في تحضير الـ standard solution أو المواد
+
+289
+00:22:13,660 --> 00:22:17,660
+الكيميائية المستخدمة في تحضير الـ standard solution
+
+290
+00:22:17,660 --> 00:22:20,780
+تكون of analytical grade إيش يعني analytical
+
+291
+00:22:20,780 --> 00:22:26,020
+grade؟ يعني على درجة عالية من الثبات والدقة و
+
+292
+00:22:26,020 --> 00:22:31,800
+النقاء لحتى أتمكن من أن أستخدمها في فحص
+
+293
+00:22:31,800 --> 00:22:36,960
+طب وأجيب تركيز العينة المجهول بدلالة هذا المعلوم
+
+294
+00:22:36,960 --> 00:22:43,660
+تمام؟ طيب Since poor standard solutions cause errors
+
+295
+00:22:43,660 --> 00:22:46,960
+on the estimation of the intended substance, their
+
+296
+00:22:46,960 --> 00:22:52,640
+accurate preparation is of paramount importance تمام؟ إيش
+
+297
+00:22:52,640 --> 00:22:56,000
+يعني الكلام هذا؟ يعني بيحكي لك إنه إذا كان الـ
+
+298
+00:22:56,000 --> 00:23:00,160
+standard solution تم تحضيره أو تخزينه أو التعامل
+
+299
+00:23:00,160 --> 00:23:05,400
+معاه بطريقة سيئة بحيث صار تالف تمام؟ هذا راح يسبب لي
+
+300
+00:23:05,400 --> 00:23:11,340
+errors أخطاء وخلل في عملية تقدير أو إيجاد تركيز
+
+301
+00:23:11,340 --> 00:23:15,920
+المواد المجهولة داخل العينة تمام importance in
+
+302
+00:23:15,920 --> 00:23:18,760
+order to obtain accurate and precise laboratory
+
+303
+00:23:18,760 --> 00:23:22,740
+findings in the medical laboratory إذن أهمية
+
+304
+00:23:22,740 --> 00:23:28,020
+standard solution تنبع من إنها هي بتمكنني من الحصول
+
+305
+00:23:28,020 --> 00:23:33,220
+على accurate and precise laboratory findings يعني
+
+306
+00:23:33,220 --> 00:23:39,540
+نتائج تمام laboratory results نتائج دقيقة وصحيحة
+
+307
+00:23:39,540 --> 00:23:42,100
+accurate and precise laboratory findings أو
+
+308
+00:23:42,100 --> 00:23:46,160
+laboratory results يعني من خلال استخدام الـ standard
+
+309
+00:23:46,160 --> 00:23:50,220
+solution ممكن أنا أُصنف هذا الـ standard solution إلى
+
+310
+00:23:50,220 --> 00:23:54,160
+نوعين أول نوع هو الـ primary standard solution
+
+311
+00:23:54,160 --> 00:23:58,460
+والنوع الثاني هو الـ secondary standard solution
+
+312
+00:23:58,460 --> 00:24:01,820
+طيب خلينا نشوف إيش يعني primary standard solution
+
+313
+00:24:02,120 --> 00:24:05,680
+الـ primary standard solution هو عبارة عن chemical
+
+314
+00:24:05,680 --> 00:24:10,140
+solution that has the highest purity and can be
+
+315
+00:24:10,140 --> 00:24:12,820
+used directly for the exact measurement of
+
+316
+00:24:12,820 --> 00:24:15,840
+substances of unknown concentration in a given
+
+317
+00:24:15,840 --> 00:24:20,000
+solution بيحكي لك أن الـ primary standard solution
+
+318
+00:24:20,000 --> 00:24:24,080
+هو عبارة عن محلول كيميائي with the highest purity
+
+319
+00:24:24,080 --> 00:24:30,720
+يعني أعلى درجات النقاء والدقة يعني الفعالية تبعته
+
+320
+00:24:31,330 --> 00:24:37,850
+فبالتالي مباشرة بقدر أنا أستخدمه في إيجاد تركيز
+
+321
+00:24:37,850 --> 00:24:41,790
+مجهول باستخدام المعلوم يعني بدلالة معلوم اللي هو
+
+322
+00:24:41,790 --> 00:24:45,990
+الـ primary standard solution من أمثلة هذه المحاليل
+
+323
+00:24:45,990 --> 00:24:50,010
+الـ sodium chloride الـ sodium bicarbonate والـ
+
+324
+00:24:50,010 --> 00:24:55,710
+potassium iodide تمام؟ طيب إيش لازم يعني أو كيف الـ
+
+325
+00:24:55,710 --> 00:24:58,210
+... إيش الخصائص اللي لازم تتوفر في الـ primary
+
+326
+00:24:58,210 --> 00:25:03,230
+standard solution بيحكي لك لازم أن هو يكون مصنوع من
+
+327
+00:25:03,230 --> 00:25:07,370
+مواد أول حاجة free of impurities يعني خالية من
+
+328
+00:25:07,370 --> 00:25:13,210
+الشوائب لازم يكون highest pure يعني نقي جدا هذا أول
+
+329
+00:25:13,210 --> 00:25:18,890
+خاصية أو أول صفة لازم يعني تتوفر في المواد المكونة
+
+330
+00:25:18,890 --> 00:25:21,650
+للـ primary standard أو المكونات للـ primary
+
+331
+00:25:21,650 --> 00:25:26,050
+standard ثاني شغلة stable on keeping in solid
+
+332
+00:25:26,050 --> 00:25:30,530
+state and in solution يعني لازم يكون هذا المحلول
+
+333
+00:25:30,530 --> 00:25:33,950
+أو المواد المكونة للـ primary أو المصنوعة أو اللي من
+
+334
+00:25:33,950 --> 00:25:38,730
+خلالها أنا ب ... بكون أو ب ... بصنع primary standard
+
+335
+00:25:38,730 --> 00:25:43,930
+solution لازم تكون هذه المواد ثابتة فيه سواء كانت
+
+336
+00:25:43,930 --> 00:25:47,450
+في الحالة الصلبة أو السائلة كيف يعني؟ يعني لو كانت
+
+337
+00:25:47,450 --> 00:25:51,470
+لسه بودر وأنا لسه ما حضرتش منها primary standard
+
+338
+00:25:51,470 --> 00:25:55,990
+solution طبعا لازم تظل هذه المادة تكون ثابتة لو
+
+339
+00:25:55,990 --> 00:25:59,090
+أنا بدأت أحطها في درجة حرارة حرارة الغرفة أو في
+
+340
+00:25:59,090 --> 00:26:04,300
+ثلاجة أو في درجة حرارة التخزين وأيضا لو أنا حضرت
+
+341
+00:26:04,300 --> 00:26:09,380
+منها محلول لازم المحلول يظل ثابت التركيز طيب إيش
+
+342
+00:26:09,380 --> 00:26:13,640
+يعني مثلا لو أنا حطيت مادة اللي هي powder بتمتص
+
+343
+00:26:13,640 --> 00:26:19,430
+رطوبة في درجة حرارة الغرفة فبالتالي هذه المادة اللي
+
+344
+00:26:19,430 --> 00:26:24,490
+بتنص رطوبة رح يتغير مكوناتها فبالتالي هي لا تصلح
+
+345
+00:26:24,490 --> 00:26:27,830
+لأنها تكون مكون من مكونات الـ primary standard
+
+346
+00:26:27,830 --> 00:26:32,370
+solution تمام هذا معناه أنه يكون stable in keeping
+
+347
+00:26:32,370 --> 00:26:35,670
+in solid state أو in solution لو أنا حضرت محلول
+
+348
+00:26:35,670 --> 00:26:39,270
+على أساس أنه هو primary standard solution واتفاجأت
+
+349
+00:26:39,270 --> 00:26:43,680
+أنه بيصير له evaporation بيتبخر معناها هذا لا يصلح
+
+350
+00:26:43,680 --> 00:26:47,960
+لا يصلح أن يكون primary standard solution هذه
+
+351
+00:26:47,960 --> 00:26:52,970
+معناها أنه يكون stable ثابت طبعا ... طبعا لازم كمان
+
+352
+00:26:52,970 --> 00:26:56,330
+يكون able to be accurately weighed or measured
+
+353
+00:26:56,330 --> 00:27:01,330
+لازم طبعا أنا أكون قادرة أن أنا أقيس وزنه أو
+
+354
+00:27:01,330 --> 00:27:06,630
+أقيسه أو أعرف تركيزه بشكل دقيق جدا لأنه أنا تركيزه
+
+355
+00:27:06,630 --> 00:27:12,610
+هذا معلوم تمام بدي استخدمه عشان أجي ... أجد
+
+356
+00:27:12,610 --> 00:27:18,720
+بدلالته تركيز مجهول موجود داخل العينة طيب لازم أيضا
+
+357
+00:27:18,720 --> 00:27:22,880
+يكون not hygroscopic إيش يعني hygroscopic يعني يمتص
+
+358
+00:27:22,880 --> 00:27:27,200
+رطوبة hygroscopic معناها يمتص رطوبة not
+
+359
+00:27:27,200 --> 00:27:32,340
+hygroscopic يعني لا يمتص رطوبة هذا شرط من شروط الـ
+
+360
+00:27:32,340 --> 00:27:35,760
+primary standard solution doesn't absorb moisture
+
+361
+00:27:35,760 --> 00:27:40,360
+and vaporize حتى كمان ما بيتبخرش على درجة حرارة
+
+362
+00:27:40,360 --> 00:27:44,600
+الغرفة أو حتى درجة حرارة 20 هذا يعني الثبات
+
+363
+00:27:44,600 --> 00:27:49,880
+الثبات وأنه يكون pure هذه الخصائص اللي لازم تتوفر
+
+364
+00:27:49,880 --> 00:27:53,420
+في الـ primary standard solution هو عبارة عن الـ
+
+365
+00:27:53,420 --> 00:27:58,740
+highest pure solution اللي أنا من خلاله بجيب تركيز
+
+366
+00:27:58,740 --> 00:28:02,840
+مجهول داخل العينة بدلالة التركيز المعلوم اللي هو
+
+367
+00:28:02,840 --> 00:28:07,240
+الـ primary standard solution بمشي زي زي العينة بضيف
+
+368
+00:28:07,240 --> 00:28:12,300
+عليهم الاثنين reagent وبراقب التغير لو صار تغير في
+
+369
+00:28:12,300 --> 00:28:15,160
+العينة ضعف التغير اللي في ... في الـ standard
+
+370
+00:28:15,160 --> 00:28:18,040
+معناها التركيز في العينة ضعف التركيز اللي في الـ
+
+371
+00:28:18,040 --> 00:28:24,740
+standard لو صار تغير في العينة ثلث التغير اللي ...
+
+372
+00:28:24,740 --> 00:28:27,880
+ثلث التغير اللي صار في الـ standard معناها التركيز
+
+373
+00:28:27,880 --> 00:28:33,820
+في العينة ثلث التركيز في الـ standard وهكذا تمام؟
+
+374
+00:28:34,900 --> 00:28:38,660
+طيب secondary standard solution الـ secondary
+
+375
+00:28:38,660 --> 00:28:44,460
+standard solution هي عبارة عن محلول أقل نقاء lower
+
+376
+00:28:44,460 --> 00:28:50,400
+purity تمام وإذا هي ما بتستخدم directly علشان
+
+377
+00:28:50,400 --> 00:28:55,440
+أنا أجيب فيه أو أستخدمها كمعلوم وأجيب يعني
+
+378
+00:28:55,440 --> 00:29:00,620
+بدلالته تركيز مجهول لأ طبعا هي ... أنا كمان
+
+379
+00:29:00,620 --> 00:29:04,460
+ما بكونش عارفة تركيز تبعها بجيبه أنا بقارنها بـ
+
+380
+00:29:04,460 --> 00:29:09,500
+primary standard تمام؟ إذا هو محلول مش كثير نقي و
+
+381
+00:29:09,500 --> 00:29:14,920
+مش معلوم تركيزه بقارنه ب ... بمحلول الـ primary
+
+382
+00:29:14,920 --> 00:29:19,000
+standard بمحلول ال ... ال ... الـ هي يعني الـ number
+
+383
+00:29:19,000 --> 00:29:23,240
+one معلومة التركيز وبعد هيك ممكن أنا أستخدمها بالـ
+
+384
+00:29:23,240 --> 00:29:26,500
+analytical procedures زي في التجارب التحليلية و
+
+385
+00:29:26,500 --> 00:29:31,970
+المعايرة والأشياء هذه طيب من الأمثلة على هذه
+
+386
+00:29:31,970 --> 00:29:35,230
+المحاليل الـ nitric acid الـ hydrochloric acid والـ
+
+387
+00:29:35,230 --> 00:29:40,890
+sulphuric acid طبعا إيش ... وأنا بأحضر secondary
+
+388
+00:29:40,890 --> 00:29:45,980
+standard solution يعني في اعتبارات يجب أن تراها أول
+
+389
+00:29:45,980 --> 00:29:49,400
+حاجة بدي أنا وأنا بأحضره لازم أستخدم analytical
+
+390
+00:29:49,400 --> 00:29:54,320
+balance ل ... ل ... لأخذ الأوزان يعني لو قال لي هذه
+
+391
+00:29:54,320 --> 00:29:58,720
+المادة الـ powder خذي منها خمسة جرام وذوبيهم مثلا
+
+392
+00:29:58,720 --> 00:30:02,520
+بمئتي مل من distilled water عشان تحصلي على secondary
+
+393
+00:30:02,520 --> 00:30:06,420
+standard solution لازم أنا عشان أوزن الخمسة جرام
+
+394
+00:30:06,420 --> 00:30:11,650
+هذول أستخدم analytical balance الميزان اللي من نوع
+
+395
+00:30:11,650 --> 00:30:16,970
+analytical إيش يعني؟ يعني ميزان حساس ودقيق جدا تمام
+
+396
+00:30:16,970 --> 00:30:20,810
+طيب dissolving the weighted substance in the
+
+397
+00:30:20,810 --> 00:30:24,610
+prescribed volume الخمسة جرام هذول حكي لي أو
+
+398
+00:30:24,610 --> 00:30:29,150
+التعليمات اللي تحكي لي أن أنا أذوبهم في 100 مل من الـ
+
+399
+00:30:29,150 --> 00:30:33,110
+solvent خلاص ماء يعني ماء ما ينفع 110 ولا 120
+
+400
+00:30:33,110 --> 00:30:38,160
+ نهائيا أنا ما ينفع ألعب بالحجوم determining the
+
+401
+00:30:38,160 --> 00:30:41,420
+exact concentration by comparing against a
+
+402
+
+445
+00:34:13,770 --> 00:34:17,910
+لو كانت الإنزيمات هذه نشاطها طبيعياً أو الـ...الـ...الـ
+
+446
+00:34:17,910 --> 00:34:22,820
+activity طبعتها فعال تمام، بحكي أنه وظائف الكبد
+
+447
+00:34:22,820 --> 00:34:27,320
+ممتازة، ووظائف الكلى ممتازة، تمام؟ طيب هذه
+
+448
+00:34:27,320 --> 00:34:30,320
+الأنزيمات، طبعاً احنا بنعرف أنه أي إنزيم بالـ...
+
+449
+00:34:30,320 --> 00:34:34,760
+بالدنيا لازم أن يشتغل عند درجة حرارة مناسبة و pH
+
+450
+00:34:34,760 --> 00:34:40,300
+مناسب، وما يكون فيه inhibitor ولا يعني معيق
+
+451
+00:34:40,300 --> 00:34:46,700
+لشغله، تمام؟ طيب، فإذا لازم أنا عشان أجيس نشاط
+
+452
+00:34:46,700 --> 00:34:52,240
+الأنزيمات هذه، أوفر الـ pH المناسب ودرجة الحرارة
+
+453
+00:34:52,240 --> 00:34:56,060
+المناسبة، لإنه الإنزيم يكون فعال عشان أجيس نشاطه
+
+454
+00:34:56,060 --> 00:35:00,540
+فبالتالي أستخدم buffers علشان أوفر الـ pH المناسب
+
+455
+00:35:00,540 --> 00:35:04,960
+لنَشاط هذا الـ enzyme، فبالتالي يكون الفحص طبيعي، شغال
+
+456
+00:35:04,960 --> 00:35:09,800
+أو الفحص طبيعي، دقيق أو معبر عن نشاط الإنزيم، ما بنفعش
+
+457
+00:35:09,800 --> 00:35:13,440
+...يعني أنا مثلاً في عندي في جسمي إنزيمات المعدة
+
+458
+00:35:13,440 --> 00:35:18,740
+بتشتغل عند الـ...الـ...الـ...البيئات الـ acidic،
+
+459
+00:35:18,740 --> 00:35:23,510
+تمام؟ وعندي إنزيمات الـ intestine اللي هي الأمعاء
+
+460
+00:35:23,510 --> 00:35:28,670
+بتشتغل عند الـ pH الـ alkaline، ما بنفع أنا أروح آخذ
+
+461
+00:35:28,670 --> 00:35:35,990
+أقيس تركيز إنزيمات المعدة وأنا حاطة الـ pH للتفاعل
+
+462
+00:35:35,990 --> 00:35:39,850
+٩ أو ١٠، طب ما المعدة أصلاً الـ pH تبعها ٣، و
+
+463
+00:35:39,850 --> 00:35:44,610
+الأنزيمات مصممة أنها تشتغل على pH ٣، ليش أنا أقيسها
+
+464
+00:35:44,610 --> 00:35:49,730
+في تفاعل الـ pH تبعه ٨ أو ٩؟ غلط، ساعتها الأنزيمات مش
+
+465
+00:35:49,730 --> 00:35:53,090
+هتشتغل، وأنا...وأنا أفكر أن الأنزيمات مضروبة
+
+466
+00:35:53,090 --> 00:35:56,930
+يعني في مشكلة في المعدة، ده، فبالتالي أبدأ أعطي نتائج
+
+467
+00:35:56,930 --> 00:36:02,250
+خاطئة، وتفسير خاطئ، وdiagnosis خاطئ، فمن...يفضل أن
+
+468
+00:36:02,250 --> 00:36:08,230
+أنا أوفر الـ pH المناسب لنشاط الـ enzyme علشان أنا
+
+469
+00:36:08,230 --> 00:36:14,010
+لما أعبر عن الـ activity تبعته، أعبر بشكل دقيق، تمام، و
+
+470
+00:36:14,010 --> 00:36:18,270
+كيف أنا بوفر الـ pH المناسب لهذا الـ enzyme باستخدام
+
+471
+00:36:18,270 --> 00:36:24,510
+الـ buffer، تمام، باستخدام الـ buffer، طيب المحاليل هذه
+
+472
+00:36:24,510 --> 00:36:29,950
+طبعاً الـ...الـ...احنا حكينا يعني أنه قصة التركيز
+
+473
+00:36:29,950 --> 00:36:34,650
+أو نقطة التركيز لهذه المحاليل مهمة جداً، طبعاً فإذا
+
+474
+00:36:34,650 --> 00:36:38,970
+لازم أنا يكون طبعاً أنه أنا أعبر عن محاليل أو تركيز
+
+475
+00:36:38,970 --> 00:36:44,130
+المحاليل بشكل accurate، طبعاً، وباستخدام الـ...الـ
+
+476
+00:36:44,130 --> 00:36:48,180
+...الـ unit المناسبة، يعني لما أنا بدي أحكي تركيز
+
+477
+00:36:48,180 --> 00:36:54,680
+المحلول هذا خمسة، طب خمسة إيش؟ ما بعرف، خمسة وخلاص
+
+478
+00:36:54,680 --> 00:36:59,180
+أصلاً ما...هذا تركيز غير معبر، لأ، لكن أحكي أنا
+
+479
+00:36:59,180 --> 00:37:05,100
+خمسة milligram per deciliter أو خمسة في المئة
+
+480
+00:37:05,100 --> 00:37:11,080
+weight volume أو خمسة molar، إذا أنا هيك فهمت إيش
+
+481
+00:37:11,080 --> 00:37:16,950
+يعني تركيز، تمام؟ مجرد ما أنا ذكرت الـ...الوحدة،
+
+482
+00:37:16,950 --> 00:37:21,370
+وحدة القياس، إذا أنا عرفت أو عبرت عن التركيز بشكل
+
+483
+00:37:21,370 --> 00:37:26,520
+كتير أفضل من ما إني أعبر عن القيمة فقط، وإذا لازم
+
+484
+00:37:26,520 --> 00:37:30,200
+أنا أعبر أو يعني يكون الـ concentration دقيق، وأعبر
+
+485
+00:37:30,200 --> 00:37:34,620
+عنه بالوحدة المناسبة له، طبعاً في عندي نوعين من
+
+486
+00:37:34,620 --> 00:37:38,620
+الوحدات اللي تستخدم في التعبير عن القياس، في وحدات
+
+487
+00:37:38,620 --> 00:37:41,820
+بُسميها physical unit، الـ physical unit إيش يعني
+
+488
+00:37:41,820 --> 00:37:46,060
+physical unit؟ لما أحكي أنه وحدات فيزيائية، إيش
+
+489
+00:37:46,060 --> 00:37:49,960
+يعني؟ يعني مثلاً باستخدام الوزن والطول والـ...
+
+490
+00:37:49,960 --> 00:37:55,060
+والحجم، هك...هيك يعني بأعبر فيزيائياً، تمام؟
+
+491
+00:37:55,060 --> 00:37:58,820
+الفيزيائي يعني أنه مثلاً هي أخذته يمكن بالـ...بالـ
+
+492
+00:37:58,820 --> 00:38:04,080
+...بالفيزياء، أنه يعني بأول محاضرة إن كانت اللي هي
+
+493
+00:38:04,080 --> 00:38:09,140
+عن الـ...الـ...الوحدات المعيارية، الـ...الـ...الوزن
+
+494
+00:38:09,140 --> 00:38:15,020
+والطول والحجم، الـ...المسافة هي الطول، اه، يعني زي
+
+495
+00:38:15,020 --> 00:38:20,260
+هيك، فإذا أنا باستخدام هاي الوحدات بأعبر عنها، فإذا
+
+496
+00:38:20,260 --> 00:38:22,920
+أنا أستخدم، بكون physical unit للتعبير عن الـ
+
+497
+00:38:22,920 --> 00:38:28,400
+concentration، أول وحدة أو أول مثال هنستخدمه اللي هو
+
+498
+00:38:28,400 --> 00:38:32,260
+الـ weight to volume، الـ weight to volume هو عبارة
+
+499
+00:38:32,260 --> 00:38:36,920
+عن إيش يعني؟ وزن الـ solute بالنسبة لحجم الـ
+
+500
+00:38:36,920 --> 00:38:41,400
+solvent، تمام؟ إذا وزن الـ solute بالنسبة لحجم الـ
+
+501
+00:38:41,400 --> 00:38:45,300
+solvent، هادي بسميها weight to volume unit، في عندي
+
+502
+00:38:45,300 --> 00:38:49,620
+محلول مثلاً، هيعطيني، بيحكي لي أنه تركيز محلول لـ
+
+503
+00:38:49,620 --> 00:38:54,400
+glucose solution هو عبارة عن أربعين جرام في المئة
+
+504
+00:38:54,400 --> 00:38:58,240
+weight to volume، أربعين جرام في المئة weight to
+
+505
+00:38:58,240 --> 00:39:02,300
+volume، هذه لو أنا بدي أفسرها إيش يعني؟ يعني مثلاً
+
+506
+00:39:02,300 --> 00:39:08,580
+أربعين جرام من الـ weight، تمام؟ أربعين جرام من الـ
+
+507
+00:39:08,580 --> 00:39:15,040
+weight لمين؟ للـ glucose، في إيش؟ في مئة مل، المئة
+
+508
+00:39:15,040 --> 00:39:18,680
+ بالمئة هذه معناها مئة مل من الـ volume
+
+509
+00:39:18,680 --> 00:39:23,600
+لمين؟ للـ solvent طبعاً؟ إذا كانت الـ...الـ...لو
+
+510
+00:39:23,600 --> 00:39:26,800
+أنا بدي أحكي أربعين جرام في المئة weight و volume
+
+511
+00:39:26,800 --> 00:39:30,720
+glucose solution، إيش معناه الكلام هذا؟ معناه أنه
+
+512
+00:39:30,720 --> 00:39:35,980
+في محلول glucose طبعاً؟ محلول سكر تم تحضيره بذابة
+
+513
+00:39:35,980 --> 00:39:39,080
+أربعين جرام، الـ weight هذا اللي هي وزن الـ...الـ
+
+514
+00:39:39,080 --> 00:39:43,580
+solute، أربعين جرام من الـ glucose، الـ powder طبعاً
+
+515
+00:39:43,580 --> 00:39:49,900
+أضفتها أو أذبتها في إيش؟ في مئة مل طبعاً، volume يعني
+
+516
+00:39:49,900 --> 00:39:57,120
+في مئة مل منين؟ من الـ...الـ solvent طبعاً، وهنا أستخدم
+
+517
+00:39:57,120 --> 00:40:00,700
+إيش؟ الـ physical unit، الـ physical unit للـ weight
+
+518
+00:40:00,700 --> 00:40:06,180
+لـ weight جرام، والـ physical unit للـ volume مل
+
+519
+00:40:06,180 --> 00:40:07,300
+طبعاً
+
+520
+00:40:11,300 --> 00:40:15,020
+طيب النوع الآخر، لما أحكي weight to weight طبعاً
+
+521
+00:40:15,020 --> 00:40:17,960
+weight...weight to weight أو weight per weight
+
+522
+00:40:17,960 --> 00:40:23,220
+طيب إيش يعني؟...يعني وزن السوليوت بالنسبة لوزن الـ
+
+523
+00:40:23,220 --> 00:40:27,440
+solvent، يعني مثلاً هي هنا مثال معطيني تلاتين جرام
+
+524
+00:40:27,440 --> 00:40:30,560
+في المئة weight to weight hydrochloric acid، إيش
+
+525
+00:40:30,560 --> 00:40:34,680
+معناها؟ معناها أنه تم تحضير هذا المحلول باستخدام
+
+526
+00:40:34,680 --> 00:40:40,140
+ثلاثين جرام من الـ hydrochloric acid، طبعاً هذا الـ...
+
+527
+00:40:40,140 --> 00:40:50,370
+السوليوت، بكمل للمئة هنا، بكمل للمئة يعني حطيت
+
+528
+00:40:50,370 --> 00:40:58,120
+سبعين جرام من الـ solvent، طبعاً فها ده بِفسر عن الـ
+
+529
+00:40:58,120 --> 00:41:01,560
+weight to volume، الـ weight to volume، الـ ٤٠ في ١٠٠
+
+530
+00:41:01,560 --> 00:41:06,140
+طبعاً، أما اللي...اللي لما...اللي لما تكون weight
+
+531
+00:41:06,140 --> 00:41:10,320
+to weight بتكون يعني ثلاثين في المئة، معناها ثلاثين
+
+532
+00:41:10,320 --> 00:41:15,840
+في سبعين، هذا الفرق ما بينهم، والـ volume per volume
+
+533
+00:41:15,840 --> 00:41:21,610
+أو الـ volume to volume ratio، معناها هنا حجم السوليوت
+
+534
+00:41:21,610 --> 00:41:26,670
+بالنسبة لحجم الـ solvent، تسعين في المئة volume to
+
+535
+00:41:26,670 --> 00:41:30,490
+volume، إيثانول، إيش معناها؟ معناها أنا حضرت إيثانول
+
+536
+00:41:30,490 --> 00:41:37,280
+solution، حطيت تسعين مل من الإيثانول على عشرة مل
+
+537
+00:41:37,280 --> 00:41:41,620
+من الـ...الـ solvent أو الـ distilled water، تمام؟
+
+538
+00:41:41,620 --> 00:41:45,780
+إذا لما بتكون الوحدات الـ ratio weight to weight
+
+539
+00:41:45,780 --> 00:41:49,780
+تمام؟ أو volume to volume، لما يكون هذا الـ ratio
+
+540
+00:41:49,780 --> 00:41:54,580
+متجانس، volume to volume هي متجانس، حجم لحجم، وزن
+
+541
+00:41:54,580 --> 00:42:00,280
+ لوزن، تمام؟ بيتم التحضير عن طريق التكميل، يعني أنا
+
+542
+00:42:00,280 --> 00:42:06,400
+بحط ثلاثين وبكمل للمئة، تمام؟ بكمل...يعني سبعين،
+
+543
+00:42:06,400 --> 00:42:10,680
+ما بحطش مش ثلاثين ومئة، لأ، ثلاثين وسبعين، بكمل،
+
+544
+00:42:10,680 --> 00:42:14,960
+تكمل، الـ volume to volume نفس الشيء، متجانس هي،
+
+545
+00:42:14,960 --> 00:42:19,420
+حطيت تسعين وكملت للمئة، ضفت عشرة فقط، لغير، لكن لما
+
+546
+00:42:19,420 --> 00:42:25,270
+يكون weight to volume، لأ، ما بكملش، هنا أربعين
+
+547
+00:42:25,270 --> 00:42:29,330
+جرام وبحط المئة...المئة في المئة هذه يعني مئة
+
+548
+00:42:29,330 --> 00:42:33,210
+مل distilled water، ما بكملش طبعاً أنا...طبعاً
+
+549
+00:42:33,210 --> 00:42:36,730
+هنا الأربعين جرام أنا مش عارفة إن هم...هم ما بجيش
+
+550
+00:42:36,730 --> 00:42:41,720
+حجمهم عشان أكمل تكميل، ما بنفعش أكمل، تمام؟ فبالتالي
+
+551
+00:42:41,720 --> 00:42:45,300
+الأربعين جرام بحط عليهم الـ volume زي ما هو، مئة في
+
+552
+00:42:45,300 --> 00:42:49,120
+المئة، لكن لما تكون الوحدة متجانسة بقى هستخدم اللي
+
+553
+00:42:49,120 --> 00:42:54,540
+هي التكميل، يعني أن أنا ب...الـ final volume، الوزن
+
+554
+00:42:54,540 --> 00:42:58,480
+النهائي، في النهاية بده يكون في عندي مئة جرام،
+
+555
+00:42:58,480 --> 00:43:03,860
+ثلاثين وسبعين، الوزن النهائي مئة، هنا تسعين وعشرة، الـ
+
+556
+00:43:03,860 --> 00:43:08,790
+final volume بده يكون مئة، تمام؟ هي معنى الـ...
+
+557
+00:43:08,790 --> 00:43:12,930
+الوحدات الفيزيائية، طبعاً في عندي وحدات كيميائية
+
+558
+00:43:12,930 --> 00:43:17,370
+اللي هي تستخدم في التركيز المحلول اللي...اللي
+
+559
+00:43:17,370 --> 00:43:22,310
+تستخدم في الـ...المختبرات، زي الـ acid والـ bases والـ
+
+560
+00:43:22,310 --> 00:43:26,770
+ reagents وأحياناً الـ standards والأشياء هذه
+
+561
+00:43:26,770 --> 00:43:32,710
+طبعاً، أحياناً بنعبر عنها بالـ specific gravity أو الـ
+
+562
+00:43:32,710 --> 00:43:36,630
+percentage by weight طبعاً، أو أحياناً بنعبر بالـ...
+
+563
+00:43:36,630 --> 00:43:41,630
+بالـ...الـ molar...الـ molarity والـ normality طبعاً
+
+564
+00:43:41,630 --> 00:43:45,970
+الـ molarity والـ normality طبعاً، طيب الـ molar
+
+565
+00:43:45,970 --> 00:43:51,620
+solution، الـ molar solution أو المحلول اللي أنا بدي
+
+566
+00:43:51,620 --> 00:43:57,000
+أعبر عنه بالـ molarity، تمام؟ أخذنا قانون مهم جداً
+
+567
+00:43:57,000 --> 00:44:00,340
+اللي هو عبارة عن، أو أخذتوها أنتو بالتحليلية، مار
+
+568
+00:44:00,340 --> 00:44:05,160
+عليكم، اللي هو عبارة عن الـ molarity تساوي الـ number
+
+569
+00:44:05,160 --> 00:44:09,940
+of moles على الـ volume per liter، تمام، الـ number of
+
+570
+00:44:09,940 --> 00:44:14,320
+moles على الـ volume per liter، هذا بسميها إيش، بجيب أنا
+
+571
+00:44:14,320 --> 00:44:18,860
+أنا الـ molarity تبعته المحلول، الـ normality، تمام،
+
+572
+00:44:18,860 --> 00:44:24,780
+solution، اللي هو عبارة عن الـ...الـ...الـ molecular
+
+573
+00:44:24,780 --> 00:44:29,830
+weight على الـ equivalent number على الـ n، تمام؟ و
+
+574
+00:44:29,830 --> 00:44:34,230
+هذه أيضاً اللي هي...اللي بسميها الـ Valency، تمام؟
+
+575
+00:44:34,230 --> 00:44:38,810
+بسميها الـ Valency، فهي الفرق ما بين الـ molarity والـ
+
+576
+00:44:38,810 --> 00:44:48,090
+normality، طبعاً، رح أصور لكم محاضرة وأنا بكتب و
+
+577
+00:44:48,090 --> 00:44:52,550
+بترك لكم يعني و ب...ب...أضرب لكم أمثلة على حساب الـ
+
+578
+00:44:52,550 --> 00:44:58,610
+molarity وحساب الـ normality، تمام؟ وراح توضح
+
+579
+00:44:58,610 --> 00:45:01,230
+الفكرة أكثر، وراح أكتب معها القوانين وتكون
+
+580
+00:45:01,230 --> 00:45:05,370
+المحاضرة مصورة عندكم وموجودة كمرجع، فإن شاء الله
+
+581
+00:45:05,370 --> 00:45:11,070
+مش راح تتصعبوا منها، مش زي ذكر المعلومة هيك ونمر
+
+582
+00:45:11,070 --> 00:45:13,730
+عنها مرور الكرام، لأ، راح يكون إن شاء الله الشيء
+
+583
+00:45:13,970 --> 00:45:18,350
+ملموس عندكم، من خلال أن أنا راح أصور محاضرة تصوير، و
+
+584
+00:45:18,350 --> 00:45:24,910
+أنا بكتب، وبأعطيكم أمثلة وأشياء حسابية، لحتى أنعمق
+
+585
+00:45:24,910 --> 00:45:30,090
+فهم إيش يعني molarity وإيش يعني normality، تمام؟
+
+586
+00:45:30,090 --> 00:45:34,330
+طيب الـ...الـ dilution of solution، تخفيف المحاليل،
+
+587
+00:45:35,510 --> 00:45:41,790
+أيضاً الجزئية هاي راح نأخذ النظر منها، وجزئية
+
+588
+00:45:41,790 --> 00:45:50,310
+التطبيق اللي هي الحسابات، أيضاً راح نتركها للمحاضرة
+
+589
+00:45:50,310 --> 00:45:54,370
+اللي...اللي راح أصورها تصوير، بحيث أنه يكون كل شيء
+
+590
+00:45:54,370 --> 00:45:58,700
+واضح إن شاء الله بالنسبة لكم، خلينا بس بالأول نشوف
+
+591
+00:45:58,700 --> 00:46:02,980
+إيش يعني dilution of solution، dilution يعني تخفيف
+
+592
+00:46:02,980 --> 00:46:07,620
+المحاليل، تخفيف المحاليل، تتذكروا أول المحاضرة إيش
+
+593
+00:46:07,620 --> 00:46:12,400
+أنا حكيت لكم معلومة، حكيت لكم إن المحاليل ممكن تجيني
+
+594
+00:46:12,400 --> 00:46:16,480
+ready to use، جاهزة للاستخدام، ممكن تجيني powder وأنا
+
+595
+00:46:16,480 --> 00:46:19,700
+أحلها، يعني أستخدم...بتكون solute وبضيف
+
+596
+00:46:19,700 --> 00:46:24,680
+solvent، تمام، وممكن تجيني concentrated، تمام، وأنا
+
+597
+00:46:24,680 --> 00:46:29,510
+بدنا نعملي لها dilution، تكون مركزة وأنا بأحضر منها
+
+598
+00:46:29,510 --> 00:46:34,370
+تخفيف أو تركيز معين، بحيث أنه يكون هو اللي يتم
+
+599
+00:46:34,370 --> 00:46:39,430
+استخدامه واعتماده في التجربة، فإذا الـ dilution هو
+
+600
+00:46:39,430 --> 00:46:45,790
+عبارة عن تخفيف التركيز أو تقليل التركيز لمحلول
+
+601
+00:46:45,790 --> 00:46:51,710
+معين، تمام، مثال تجربة عندك في البيت، لو جبتي كاسة ماء
+
+602
+00:46:51,710 --> 00:46:59,550
+وحطيتي فيها معلقة سكر، معلقة صغيرة سكر، وحطيتي نص
+
+603
+00:46:59,550 --> 00:47:05,630
+الكاسة، عبيتيها ماء، ضوبت السكر منيح وتذوقتي الحل،
+
+604
+00:47:05,630 --> 00:47:11,570
+تبعه، هيكون حلو، مضبوط، حلو، تمام؟ طيب لنفرض أنه إنتي
+
+605
+00:47:11,570 --> 00:47:17,590
+كملتي الكاسة للآخر، ودوبتي منيح، عملتي إذابة منيح، و
+
+606
+00:47:17,590 --> 00:47:23,210
+تذوقتي منه، إيش راح نلاحظ؟ راح نلاحظ إنه مذاق الحلا
+
+607
+00:47:23,210 --> 00:47:30,010
+صار أقل، أخف، ليش؟ لإنه تركيز السكر صار أقل، تمام؟ و
+
+608
+00:47:30,010 --> 00:47:34,410
+هذا اللي بيشرح تعريف الـ dilution، الـ dilution هو
+
+609
+00:47:34,410 --> 00:47:40,630
+عملية، تمام؟ العملية اللي من خلالها الـ concentration
+
+610
+00:47:40,630 --> 00:47:45,530
+تركيز أو الـ activity أو النشاط لمحلول معين
+
+667
+00:52:14,530 --> 00:52:20,510
+اللي هو واحد اسمه simple dilution تمام؟ واللي هو
+
+668
+00:52:20,510 --> 00:52:24,790
+يعني مجرد مثلاً أنا آخذ من مادة مركزة حجم معين و
+
+669
+00:52:24,790 --> 00:52:28,450
+أضيف عليه حجم آخر من الـ solvent وهيك أنا عملت
+
+670
+00:52:28,450 --> 00:52:32,580
+simple dilution تمام؟ أما الـ serial dilution هو
+
+671
+00:52:32,580 --> 00:52:36,840
+عبارة عن multiple و progressive dilution يعني مثلاً
+
+672
+00:52:36,840 --> 00:52:40,220
+أنا كان عندي مادة مركزة أخذت منها حجم ووضفت solvent
+
+673
+00:52:40,220 --> 00:52:44,500
+في أنبوبة رقم واحد، بعدين أخذت من أنبوبة رقم واحد
+
+674
+00:52:44,500 --> 00:52:48,300
+حجم معين وحطيته في أنبوبة رقم اثنين ووضفت عليها
+
+675
+00:52:48,300 --> 00:52:53,660
+solvent، بعدين أخذت حجم من أنبوبة رقم اثنين ووضفته في
+
+676
+00:52:53,660 --> 00:52:56,880
+أنبوبة رقم ثلاثة ووضفت عليها solvent ف أنا أُشْ ما
+
+677
+00:52:56,880 --> 00:53:02,340
+أعمله بخفف أكثر وأكثر وأكثر من الـ... المادة
+
+678
+00:53:02,340 --> 00:53:07,160
+المراد عمل تخفيف لها ومن استخدام إيش؟
+
+679
+00:53:07,160 --> 00:53:10,080
+بالاستخدام multiple dilutions أنا استخدمت tube برقم
+
+680
+00:53:10,080 --> 00:53:13,400
+واحد، بعد tube برقم اثنين، بعد tube برقم ثلاثة وهكذا
+
+681
+00:53:13,400 --> 00:53:17,080
+فبالتالي أنا حصلت على multiple dilutions، ففي
+
+682
+00:53:17,080 --> 00:53:21,500
+النهاية الـ... هذا بسميه serial dilution اللي هو
+
+683
+00:53:21,500 --> 00:53:25,880
+التخفيف المتسلسل من tube رقم واحد للاثنين لثلاثة و
+
+684
+00:53:25,880 --> 00:53:33,710
+هكذا، طيب وأيضاً ممكن أنا أستخدمه علشان أنا لو كان بـ
+
+685
+00:53:33,710 --> 00:53:38,190
+... لو كان عندي... بدي محاليل كثيرة تركيزها قليل أو
+
+686
+00:53:38,190 --> 00:53:41,970
+لأن أنا لو بدي أحصل على محاليل بـ... في... يعني
+
+687
+00:53:41,970 --> 00:53:47,870
+الفروق ما بينها ثابتة في الـ... التركيز تمام؟ هو
+
+688
+00:53:47,870 --> 00:53:51,910
+عبارة عن multiple حكينا، والـ total dilution هو the
+
+689
+00:53:51,910 --> 00:53:55,490
+product of each dilution in the series، هو الـ total
+
+690
+00:53:55,490 --> 00:54:00,690
+dilution، يعني التخفيف النهائي هو مجموع ضرب كل الـ
+
+691
+00:54:00,690 --> 00:54:04,930
+dilution تبع الأنابيب كلها، هنحكي إيش يعني بالظبط
+
+692
+00:54:04,930 --> 00:54:10,590
+تمام، الـ serial dilution بيبدأ في البداية بيكون
+
+693
+00:54:10,590 --> 00:54:14,210
+عبارة عن simple، يعني أنا عندي stock تمام، أخذت منه
+
+694
+00:54:14,210 --> 00:54:17,830
+حجم وحطيت عليه solvent، فـ أنبوبة رقم واحد، الأنبوبة رقم
+
+695
+00:54:17,830 --> 00:54:23,420
+واحد لحالها هي بسميها simple dilution تمام، وبعد
+
+696
+00:54:23,420 --> 00:54:27,200
+هيك التخفيفات الثانية اللي في أنبوبة رقم اثنين و
+
+697
+00:54:27,200 --> 00:54:30,740
+ثلاثة وهكذا، هدول إيش معنى لهم بسميهم subsequent
+
+698
+00:54:30,740 --> 00:54:38,260
+dilutions، ويعني تخفيفات متتابعة، أنبوبة رقم واحد وما
+
+699
+00:54:38,260 --> 00:54:43,180
+يليها كلهم مع بعضهم بسميهم serial dilution تمام
+
+700
+00:54:43,180 --> 00:54:48,120
+بسميهم serial dilution، it has been known that if
+
+701
+00:54:48,120 --> 00:54:52,280
+we use larger volume we obtain a more accurate
+
+702
+00:54:52,280 --> 00:54:58,620
+dilution، إذا كان... إذا أنا استخدمت أحجام أكثر يعني
+
+703
+00:54:58,620 --> 00:55:02,640
+أحجام أكبر، بحصل على accurate dilution، يعني مثلاً لو
+
+704
+00:55:02,640 --> 00:55:09,960
+في النهاية لو أنا بدي أحصل على يعني بدي واحد لألف
+
+705
+00:55:09,960 --> 00:55:16,940
+واحد لألف dilution يعني آخذ one microliter وأحطهم على تسعمئة وتسعة وتسعين microliter ما ينفعش
+
+706
+00:55:16,940 --> 00:55:22,100
+واحد microliter، يعني الحجم لا يكاد يُرى بالعين
+
+707
+00:55:22,100 --> 00:55:25,600
+المجردة تمام؟ فغلط، طب أنا ليش ما أُضاعِف الكمية؟
+
+708
+00:55:25,600 --> 00:55:30,930
+آخذ مثلاً واحد مل بدل الـ microliter وأحطه في
+
+709
+00:55:30,930 --> 00:55:34,410
+تسعمئة وتسعة وتسعين مل، هيك بيكون أحسن، إذا أنا
+
+710
+00:55:34,410 --> 00:55:37,870
+لازم لما بدي أصح أنا بكون بدي أستخدم أحجام قليلة في
+
+711
+00:55:37,870 --> 00:55:42,410
+التفاعلات تبعتي، لكن أُحَضِّر الـ dilution بكميات أكبر
+
+712
+00:55:42,410 --> 00:55:46,370
+بيكون التفاعل أضبط أو يعني التركيز اللي أنا بحصل
+
+713
+00:55:46,370 --> 00:55:50,070
+عليه أو الـ dilution اللي بحصل عليه أكثر دقة تمام
+
+714
+00:55:50,070 --> 00:55:54,090
+لما بدي أعمل performing serial dilution in the
+
+715
+00:55:54,090 --> 00:55:57,190
+clinical laboratory, one must know the dilution of
+
+716
+00:55:57,190 --> 00:56:01,710
+each tube، لازم أعرف التركيز لكل أنبوبة داخل الـ...
+
+717
+00:56:01,710 --> 00:56:07,270
+الـ... العينة تبعتي، طيب وبالتالي هذا طبعاً هدول الـ
+
+718
+00:56:07,270 --> 00:56:16,630
+... هدول الأمثلة، أنا إن شاء الله راح أصور محاضرة و
+
+719
+00:56:16,630 --> 00:56:20,770
+أنا بشرح لكم كيف أنا بحصل على التركيز لـ... لـ...
+
+720
+00:56:20,770 --> 00:56:27,230
+يعني اسمه simple dilution والتركيز كيف أنا أيضاً
+
+721
+00:56:27,230 --> 00:56:33,760
+برضه بحصل عليها لـ serial dilution، يعني كيف أنا
+
+722
+00:56:33,760 --> 00:56:37,200
+برضه بحصل عليها  لـ serial dilution، يعني كيف أنا
+
+723
+00:56:37,200 --> 00:56:40,160
+أطبق لو أجاتكم مسائل على الـ primary dilution أو
+
+724
+00:56:40,160 --> 00:56:42,820
+اللي هو الـ simple dilution أو على الـ serial
+
+725
+00:56:42,820 --> 00:56:46,500
+dilution، كيف أنتم تشتغلوا وهنحكي عن الأمثلة هذه
+
+726
+00:56:46,500 --> 00:56:52,710
+ونتناولها بشكل دقيق، يعني أفضل لما أكون أنا بشرح و
+
+727
+00:56:52,710 --> 00:56:56,310
+بكتب بنفس الوقت أفضل من ما إني بس أُشير عليها بالـ
+
+728
+00:56:56,310 --> 00:57:01,290
+mouse علشان يكون هيك عمقنا الفهم عندكم أكثر، لذا
+
+729
+00:57:01,290 --> 00:57:04,790
+هذه المحاضرة يتبعها محاضرة مصورة إن شاء الله إن
+
+730
+00:57:04,790 --> 00:57:10,190
+تدرسونها راح... إن شاء الله راح تظهر لعندكم و
+
+731
+00:57:10,190 --> 00:57:14,330
+بالتالي يعني راح أحملها لكم على drive وبالتالي
+
+732
+00:57:14,330 --> 00:57:18,910
+تدرسونهم الإثنين مع بعض علشان تتضح الصورة لديكم
+
+733
+00:57:19,510 --> 00:57:25,570
+يعطيكم العافية وإن شاء الله بنشوفكم يعني أو
+
+734
+00:57:25,570 --> 00:57:29,130
+بننزل لكم المحاضرة المصورة قريباً
diff --git a/PL9fwy3NUQKwY9jl8xw-bWkVZkWcGGyspd/6stpniKSY70_postprocess.srt b/PL9fwy3NUQKwY9jl8xw-bWkVZkWcGGyspd/6stpniKSY70_postprocess.srt
new file mode 100644
index 0000000000000000000000000000000000000000..f617d8a925e886cc574fcc522c9ea37b680060e0
--- /dev/null
+++ b/PL9fwy3NUQKwY9jl8xw-bWkVZkWcGGyspd/6stpniKSY70_postprocess.srt
@@ -0,0 +1,2936 @@
+1
+00:00:04,390 --> 00:00:07,410
+السلام عليكم ورحمة الله وبركاته عزيزات الطالبات
+
+2
+00:00:07,410 --> 00:00:13,150
+معكم ان شاء الله في المحاضرة نظرية رقم اتنين لمساق
+
+3
+00:00:13,150 --> 00:00:17,830
+مقدمة في التحاليل التدبية ان شاء الله اليوم راحنا
+
+4
+00:00:17,830 --> 00:00:22,790
+ناخد مختصر او نبدأ عن ال solutions المحاليل اللي
+
+5
+00:00:22,790 --> 00:00:26,810
+بتستخدم في المختبرات التدبية solutions used in
+
+6
+00:00:26,810 --> 00:00:30,590
+medical laboratory المحاليل اللي بتستخدم في
+
+7
+00:00:30,590 --> 00:00:36,270
+المختبرات التدبيةبداية بدنا نعرف إيش يعني solution
+
+8
+00:00:36,270 --> 00:00:40,090
+solution كمحلول هي عبارة عن هو عبارة عن
+
+9
+00:00:40,090 --> 00:00:43,950
+homogeneous mixture هو عبارة عن خليط متجانس من
+
+10
+00:00:43,950 --> 00:00:47,550
+مكونين أو أكثر طبعا إذا هو عبارة عن homogeneous
+
+11
+00:00:47,550 --> 00:00:52,910
+mixture of two or more substancesطبعا طيب كمحلول
+
+12
+00:00:52,910 --> 00:00:58,590
+لابد انه يحتوي او يتكون من شغلتان solute اللي هي
+
+13
+00:00:58,590 --> 00:01:03,970
+الأشياء اللي تم تدويبها في المحلول و ال solvent
+
+14
+00:01:03,970 --> 00:01:09,390
+طبعا فال soluteهو عبارة عن ال dissolved substance
+
+15
+00:01:09,390 --> 00:01:13,870
+المادة المذابة whereas solvent هو is a substance
+
+16
+00:01:13,870 --> 00:01:17,230
+in which solute dissolved to make the mixture اذا
+
+17
+00:01:17,230 --> 00:01:21,970
+المحلول هو عبارة عن خليط من مكونات او اكثر
+
+18
+00:01:21,970 --> 00:01:26,690
+والمكونات هذه ممكن تنقسم الى اما تكون solute يعني
+
+19
+00:01:26,690 --> 00:01:32,510
+اشياء تم تذوبها تمام اشياء مذابة و solvent المذيب
+
+20
+00:01:32,510 --> 00:01:39,720
+هو المادة اللى ال solute تذوب فيهتمام ففي عندي
+
+21
+00:01:39,720 --> 00:01:43,560
+أنواع .. يعني أنواع مختلفة من المحاليل اللي تستخدم
+
+22
+00:01:43,560 --> 00:01:47,060
+في ال medical laboratory و بتجارب اللي بنشريها في
+
+23
+00:01:47,060 --> 00:01:52,180
+داخل المختبر الطبي من خلال او من ضمن هذه المحاليل
+
+24
+00:01:52,180 --> 00:01:56,480
+يعني ممكن احنا نصنفها ل reagent solutionنوع من
+
+25
+00:01:56,480 --> 00:02:00,480
+أنواع المحلي اللي بسميها reagent solution نوع أخر
+
+26
+00:02:00,480 --> 00:02:04,900
+بسميه staining solution و standard solution and
+
+27
+00:02:04,900 --> 00:02:08,420
+buffer solution راح ناخد كل واحدة على حدة و نعرف
+
+28
+00:02:08,420 --> 00:02:11,240
+إيش يعني إيش يعني reagent إيش يعني staining
+
+29
+00:02:11,240 --> 00:02:15,500
+solution standard and buffer solution خلينا نبلش
+
+30
+00:02:15,500 --> 00:02:18,980
+بأول واحدة اللي هي reagent solutionsإيش يعني رأيها
+
+31
+00:02:18,980 --> 00:02:23,140
+genre solution؟ طبعا إحنا عارفين إنه بس نجري فحص
+
+32
+00:02:23,140 --> 00:02:27,360
+طبي على عينة مريض لابد إنه مثلا على فرض إن أنا بدي
+
+33
+00:02:27,360 --> 00:02:30,960
+أقيس مستوى السكر في الدم طبعا أنا بدي أقيس ال
+
+34
+00:02:30,960 --> 00:02:34,420
+glucose مستوى السكر في الدم اللي هو ال glucose طيب
+
+35
+00:02:35,150 --> 00:02:39,090
+لابد من إنه يكون في عندي مادة متفاعلة تتفاعل مع ال
+
+36
+00:02:39,090 --> 00:02:44,170
+glucose في الدم علشان في النهاية تعطيني إما لون أو
+
+37
+00:02:44,170 --> 00:02:49,330
+تفاعل يعني هيصير في change هيصير في تغير معين أنا
+
+38
+00:02:49,330 --> 00:02:53,770
+بقدر يعني أقيس هذا التغير وبالتالي هذا التغير
+
+39
+00:02:53,770 --> 00:02:58,210
+بيعبرلي عن تركيز ال glucose في الدمنرجع تاني نعيد
+
+40
+00:02:58,210 --> 00:03:02,790
+نحكي أنه على فرض أنا بدي أقيس مستوى السكر في الدم
+
+41
+00:03:02,790 --> 00:03:07,450
+لابد من وجود مادة متفاعلة تتفاعل مع السكر هذا مع
+
+42
+00:03:07,450 --> 00:03:12,110
+ال glucose و بتعطيني تغير معين التغير هذا ممكن
+
+43
+00:03:12,110 --> 00:03:16,370
+يكون تغير في اللون ممكن يكون تساعد غازات ممكن يكون
+
+44
+00:03:16,510 --> 00:03:21,410
+ممكن يكون ظهور فقاعات ممكن يكون يعني أي نوع من
+
+45
+00:03:21,410 --> 00:03:24,370
+التغير حتى يعني رائحة بس احنا مابنعتمد كتير على
+
+46
+00:03:24,370 --> 00:03:28,650
+الروائح والاشياء هذه احنا دايما التغيرات بتكون
+
+47
+00:03:28,650 --> 00:03:33,990
+تغير في اللون او حتى ظهور غاز او تساعد غازات او
+
+48
+00:03:33,990 --> 00:03:39,650
+فقاعات يعني اشياءيمكن قياسها التغير يمكن قياسه
+
+49
+00:03:39,650 --> 00:03:43,950
+تمام بمجرد تفاعل هذا ال reagent او المادة
+
+50
+00:03:43,950 --> 00:03:49,790
+المتفاعلة مع ال glucose حدوث تغير لابد ان يكون
+
+51
+00:03:49,790 --> 00:03:54,490
+التغير هذا يمكن قياسه بحيث انه يعبرلي عن مستوى
+
+52
+00:03:54,490 --> 00:03:58,630
+السكر في الدم يعني لو فرضنا ان المادة المتفاعلة
+
+53
+00:03:58,630 --> 00:04:01,590
+اللي هتتفاعل مع هذا ال glucose بتعطيني لون pink
+
+54
+00:04:02,140 --> 00:04:07,500
+طبعا لون pink فخلينا نفرض مثلا كل ما زادت شدة
+
+55
+00:04:07,500 --> 00:04:12,900
+اللون كل ما زاد ال pink طبعا هيعطين .. هي .. هيخطر
+
+56
+00:04:12,900 --> 00:04:18,880
+في دهني انه في عندي كمية كبيرة من الاتفاعل صارت
+
+57
+00:04:18,880 --> 00:04:23,480
+يعني كمية كبيرة من ال glucose اتفاعلت مع ال agent
+
+58
+00:04:23,480 --> 00:04:29,550
+و أعطتني اللون ال pink فبالتاليإذا التغير في اللون
+
+59
+00:04:29,550 --> 00:04:33,870
+هذا اللي انا قدرت أقيصه قدرت أعرف إيش شدت اللون
+
+60
+00:04:33,870 --> 00:04:39,790
+تمام عكسلي جدش في عندي تركيز في العينة تمام هذا
+
+61
+00:04:39,790 --> 00:04:43,110
+كتقديم لل reaction solution خلينا نشوف إيش مكتوب
+
+62
+00:04:43,110 --> 00:04:47,630
+عندنا بال reaction solutionهو عبارة عن أي محلول
+
+63
+00:04:47,630 --> 00:04:52,470
+يمكن يستخدمه that is used in conjunction with a
+
+64
+00:04:52,470 --> 00:04:57,770
+given sample يمكن يتم استخدامه مع عينة مع العينة
+
+65
+00:04:57,770 --> 00:05:02,490
+تمام و بالتالي expected to produce a measurable or
+
+66
+00:05:02,490 --> 00:05:06,630
+noticable change هيصير مجلد ما يتفاعل مع مكون من
+
+67
+00:05:06,630 --> 00:05:11,210
+مكونات العينة هذا ال reaction هيحدث measurable or
+
+68
+00:05:11,210 --> 00:05:16,350
+noticable changeيعني هيحدث تغير يمكن ملاحظته او
+
+69
+00:05:16,350 --> 00:05:21,810
+يمكن قياسه فهذا المحلول اللي بيحققل الشرط هذا انه
+
+70
+00:05:21,810 --> 00:05:26,370
+بيتفاعل مع مكون من مكونات العينة وبيحدث تغير
+
+71
+00:05:26,370 --> 00:05:30,770
+التغير هذا هيحدث تغير على العينة يمكن قياسه او
+
+72
+00:05:30,770 --> 00:05:35,610
+ملاحظته على طول بسمي هذا المحلول عبارة عن reagent
+
+73
+00:05:35,610 --> 00:05:40,050
+solution تمام؟ طيب وانا بشتغل طبعا ال reagent
+
+74
+00:05:40,050 --> 00:05:44,370
+solution هاي المحاليل المتفاعلةلما ب .. أنا بطلبها
+
+75
+00:05:44,370 --> 00:05:49,490
+من الشركات طبعا كل نوع فحص يلو reagent خاص فيه،
+
+76
+00:05:49,490 --> 00:05:52,910
+يعني لما بدي أقيس مستوى الدهون في الجسم ب .. ب ..
+
+77
+00:05:52,910 --> 00:05:56,150
+بستخدم reagent خاص في الكشف عن الدهون، لما بدي
+
+78
+00:05:56,150 --> 00:06:00,750
+أقيس مستوى السكر في الدم بستخدم reagent متخصص في
+
+79
+00:06:00,750 --> 00:06:05,060
+الكشف عن ال glucoseلو بدي اقيس مستوى بروتينات بدي
+
+80
+00:06:05,060 --> 00:06:08,900
+استخدم reagent متخصص في الكشف عن البروتينات نشاط
+
+81
+00:06:08,900 --> 00:06:12,320
+انزيمات بستخدم reagent متخصص في الكشف عن نشاط
+
+82
+00:06:12,320 --> 00:06:16,860
+الانزيمات و هكذا اذا هذه ال reagents انا بشتريها
+
+83
+00:06:16,860 --> 00:06:21,340
+من شركات خاصة هي بتصمملي الفحص يعني هي بتصمم هذا
+
+84
+00:06:21,340 --> 00:06:25,720
+ال reagent و بت .. بتعرف تركيبه الكيميائي و بتصممه
+
+85
+00:06:25,720 --> 00:06:30,280
+على اساس ان هو يكون متخصص لإجراء فحص طبي معين طيب
+
+86
+00:06:30,670 --> 00:06:34,830
+هذا ال reagent لما انا بشتريه من الشركة ممكن يكون
+
+87
+00:06:34,830 --> 00:06:40,050
+جاهز ready to use تمام ممكن يكون جاهز ready to use
+
+88
+00:06:40,050 --> 00:06:44,570
+او ممكن يكون عبارة عن powder و انا بدي احله بدي
+
+89
+00:06:44,570 --> 00:06:47,830
+احوله لمحلول يعني بدي اضيف عليه solvent ال solvent
+
+90
+00:06:47,830 --> 00:06:51,430
+هذا ممكن تكون ميه distilled water تمام او اي محلول
+
+91
+00:06:51,430 --> 00:06:56,570
+بتوصي فيه الشركةفإذا بيجيني powders solute بيكون
+
+92
+00:06:56,570 --> 00:07:00,370
+solute تمام و أنا بضيف عليه solvent عشان أكون
+
+93
+00:07:00,370 --> 00:07:06,130
+solution تمام أو ممكن يجيني مركز و أنا أخففه بحيث
+
+94
+00:07:06,130 --> 00:07:10,590
+أحصل على التخفيف المناسب للتجربة تبعتي أو للتحص
+
+95
+00:07:10,590 --> 00:07:17,730
+تبعي تمام أصبح في عندي necessary care اهتمام و
+
+96
+00:07:17,730 --> 00:07:24,610
+انتباه أثناء تحضير هذا ال reagentلازم يكون في عندي
+
+97
+00:07:24,610 --> 00:07:28,150
+care اهتمام including the following should be
+
+98
+00:07:28,150 --> 00:07:31,750
+taken in preparing a given reagent solution و انا
+
+99
+00:07:31,750 --> 00:07:35,830
+بحضر reagent solution لازم يكون في عندي انتباه
+
+100
+00:07:35,830 --> 00:07:40,770
+يستدعي انه انا احافظ على الأشياء التالية ايش هي
+
+101
+00:07:40,770 --> 00:07:43,110
+ايش اللي بده احافظ عليه؟ اول حاجة ال chemical
+
+102
+00:07:43,110 --> 00:07:47,740
+selectionاش يعني chemical selection يعني انا اختار
+
+103
+00:07:47,740 --> 00:07:51,400
+ال reagent الكيميائي المناسب لفحصي اذا انا بدي
+
+104
+00:07:51,400 --> 00:07:54,960
+اقيس بروتينات مابنفع استخدم reagent بيكشف عن وجود
+
+105
+00:07:54,960 --> 00:07:59,180
+مثلا السكر في الدم لازم يكون ايش ال reagent مختص
+
+106
+00:07:59,180 --> 00:08:02,900
+بيه الفحص تبعي و احنا سبق و حكينا انه كل reagent
+
+107
+00:08:02,900 --> 00:08:07,540
+بيكون مصمم لاجراء فحص معين فانا لازم اكون عندى ال
+
+108
+00:08:07,540 --> 00:08:12,390
+selection تبع الاختيار لهذا ال reagentختيار المادة
+
+109
+00:08:12,390 --> 00:08:15,790
+الكيميائية المناسبة لهذا ال reagent بحيث انه
+
+110
+00:08:15,790 --> 00:08:19,670
+اتناسب الفحص الطبيعي تمام؟ و اذا مابنفع انا يكون
+
+111
+00:08:19,670 --> 00:08:23,950
+عندي فحص بدي اجيس مثلا مستوى الدهون في الجسم و
+
+112
+00:08:23,950 --> 00:08:28,430
+اروح اختار اعمل selection ل reagent المادة
+
+113
+00:08:28,430 --> 00:08:31,190
+الكيميائية اللى فيه متخصصة في الكشف عن البروتينات
+
+114
+00:08:31,190 --> 00:08:35,750
+مش الدهون غلطساعتها الفحص الطبيعي هيكون خاطئ انا
+
+115
+00:08:35,750 --> 00:08:40,930
+مش هاخد نتيجة على مستوى الدهون هاخد نتيجة على
+
+116
+00:08:40,930 --> 00:08:46,190
+مستوى البروتينات وهذا غلط طيب الشغل التاني اللي
+
+117
+00:08:46,190 --> 00:08:49,290
+بدي أنتبهلها following instruction of preparation
+
+118
+00:08:49,290 --> 00:08:54,290
+ذاكرين لما حكينا بمحاضرة ال safety ان كل مادة
+
+119
+00:08:54,290 --> 00:09:01,950
+كيميائية بيكون عليها label بتكون معلمة label عليها
+
+120
+00:09:04,780 --> 00:09:08,880
+بالاسم والتركيبة الكيميائية وال expiration date
+
+121
+00:09:08,880 --> 00:09:13,940
+اللي هو تاريخ الانتهاء الصلاحية و .. يعني خطوات
+
+122
+00:09:13,940 --> 00:09:18,040
+أثناء تحضير هذا المحلول أو يعني تعليمات لتحضيره
+
+123
+00:09:18,040 --> 00:09:22,020
+تعليمات لاستخدامه تعليمات لمحافظة على سلامتنا
+
+124
+00:09:22,020 --> 00:09:26,160
+أثناء استخدامه ممكن يكون عليه علامات hazard
+
+125
+00:09:26,160 --> 00:09:29,940
+symbolsإشارات على هذا ال reagent أو هذه المادة
+
+126
+00:09:29,940 --> 00:09:32,680
+الكيميائية بحيث أنها تتنبهني هذا في acid، هذا
+
+127
+00:09:32,680 --> 00:09:39,500
+حارق، هذا ممكن يعملي تطير منه غازات و يسبب اختناق،
+
+128
+00:09:39,500 --> 00:09:43,560
+هذا ممكن ي .. تطلع منه إشعاعاتفهذه كلها بتكون
+
+129
+00:09:43,560 --> 00:09:47,480
+موجودة وين على ال label فأنا لازم أقرأ ال label
+
+130
+00:09:47,480 --> 00:09:55,340
+كتير منيح و أنتبه و أتبع تعليمات التحضير جيدا ليش؟
+
+131
+00:09:55,340 --> 00:10:00,020
+لإن أنا بدي أحضره بجودة عالية علشان أحصل على
+
+132
+00:10:00,020 --> 00:10:06,060
+reagentتركيزه دقيق فبالتالي يفحصلي او يشتغلي الفحص
+
+133
+00:10:06,060 --> 00:10:11,400
+بشكل دقيق عشان احصل على نتائج دقيقة وبالتالي اساهم
+
+134
+00:10:11,400 --> 00:10:16,580
+في ال accurate diagnosis و ال .. ال valuable ايش
+
+135
+00:10:16,580 --> 00:10:23,020
+treatment في التشخيص الدقيق و العلاج الفعال تمام؟
+
+136
+00:10:24,090 --> 00:10:28,050
+طيب، إذا الخطوة التالتة أو الشغل التالتة اللي أنا
+
+137
+00:10:28,050 --> 00:10:31,130
+بدي أنتبهلها أثناء تحضير ال reagent أنه أنا بدي
+
+138
+00:10:31,130 --> 00:10:36,170
+أستخدم using accurate measurement of substance،
+
+139
+00:10:36,170 --> 00:10:41,330
+تمام؟ حكينا إنه اللازم أكون منتبهة كتير على
+
+140
+00:10:41,330 --> 00:10:46,770
+الحجوم، جداش مثلا إذا طلع .. حكيلي مثلا خуди من
+
+141
+00:10:46,770 --> 00:10:54,010
+هذا المادة خمسة صنطي و اخلطيهم بلتر ميهتمام؟ بنفعش
+
+142
+00:10:54,010 --> 00:10:59,620
+أنا أخلطهم ب letter و ربع أو letter و نصغلط لازم
+
+143
+00:10:59,620 --> 00:11:02,980
+انا اعمل accurate measurement of substances لازم
+
+144
+00:11:02,980 --> 00:11:07,800
+انا يكون عندي احضره بحجوم دقيقة علشان احصل على
+
+145
+00:11:07,800 --> 00:11:12,340
+تركيز دقيق وبالتالي الشغل يكون كمان دقيق using of
+
+146
+00:11:12,340 --> 00:11:15,700
+appropriate type of glass or plasticware اذا هذا
+
+147
+00:11:15,700 --> 00:11:20,420
+المحلول انا بده احضره بعدك بده احطه في bottle لازم
+
+148
+00:11:20,420 --> 00:11:23,980
+ال bottle تكون مناسبة للحفاظ على ال reagent كده
+
+149
+00:11:23,980 --> 00:11:28,680
+يعنيفي reagentات و هيمر معاكم خلال رحلتكم في دراسة
+
+150
+00:11:28,680 --> 00:11:33,880
+التحاليل الطبية هيمر معاكم أنواع من ال reagents
+
+151
+00:11:33,880 --> 00:11:40,580
+يفضل حفظها ب dark bottle يعني هي حساسة للضوء و
+
+152
+00:11:40,580 --> 00:11:45,080
+لازم انا احطها في bottle لونها بني او أسود هيمر
+
+153
+00:11:45,080 --> 00:11:50,970
+معانا مثلا ال acid و ..و ال acid و ال .. و ال base
+
+154
+00:11:50,970 --> 00:11:55,970
+لازم أحطهم في glass container بس لازم الغطايا
+
+155
+00:11:55,970 --> 00:11:59,350
+تبعته ما تكون glass خدنا هذه المعلومة المحاضرة
+
+156
+00:11:59,350 --> 00:12:02,190
+الماضية حكينا أنه مش لازم تكون ال container ..
+
+157
+00:12:02,190 --> 00:12:07,990
+الغطايا glass علشان ما ت .. يعني تعلق و يصعب فتحه
+
+158
+00:12:08,540 --> 00:12:13,100
+تمام؟ في بعض المحليين لازم أحطها في plastic bottle
+
+159
+00:12:13,100 --> 00:12:17,540
+حسب فبالتالي نوع ال reagent هو اللي بيحكملي أو
+
+160
+00:12:17,540 --> 00:12:20,800
+تركيبته الكيميائية هي اللي بتحكملي ال bitter أو ال
+
+161
+00:12:20,800 --> 00:12:26,780
+best container لإلهطبعا هذا بالنسبالي ال reaction
+
+162
+00:12:26,780 --> 00:12:29,040
+solution اذا اللي بدي اعرفه عن ال reaction
+
+163
+00:12:29,040 --> 00:12:33,120
+solution ان هو عبارة عن المادة المتفاعلة مع العينة
+
+164
+00:12:33,120 --> 00:12:36,820
+اللي من شأنها تعطيني change على العينة تغير على
+
+165
+00:12:36,820 --> 00:12:42,860
+العينة يمكن قياسه ويمكن ملاحظته بحيث ان انا بعد
+
+166
+00:12:42,860 --> 00:12:48,640
+هيك احصل على قراءات او احصل على نتيجة طبعا و لازم
+
+167
+00:12:48,640 --> 00:12:54,470
+انا استدعي الانتباه و الاهتمام اثناءاختيار نوع ال
+
+168
+00:12:54,470 --> 00:12:59,210
+reagent يكون ملائم لنوع الفحص اللى بدنا احنا بصدد
+
+169
+00:12:59,210 --> 00:13:04,550
+إجراءه و أيضا بدي أتبع تعليمات أثناء تحضيره
+
+170
+00:13:04,550 --> 00:13:08,010
+تعليمات الشركة المصنية على هذا ال reagent بدي
+
+171
+00:13:08,010 --> 00:13:12,730
+أصنعه بكميات و بأحجام دقيقة بحيث أن أنا أظل محافظة
+
+172
+00:13:12,730 --> 00:13:17,030
+على تركيزه المناسب للفحصو أيضا أحطه في ال bottle
+
+173
+00:13:17,030 --> 00:13:20,350
+أو ال container المناسبة لإله بحيث أني انا أضل
+
+174
+00:13:20,350 --> 00:13:25,030
+أحافظ على فعاليته و أحافظ على خصائصه ما تتغير
+
+175
+00:13:25,030 --> 00:13:29,790
+تمام؟ و حكينا أن هذا ال reagent بيجي من شركات كل
+
+176
+00:13:29,790 --> 00:13:33,670
+reagent بيكون خاص بفحص معين ممكن يجيني powder
+
+177
+00:13:33,670 --> 00:13:36,610
+عبارة عن solute و أنا بدي أضيفله solvent عشان
+
+178
+00:13:36,610 --> 00:13:42,080
+أحوله ل solutionممكن يكون عبارة عن محاليل مركزة و
+
+179
+00:13:42,080 --> 00:13:46,060
+أنا لازم أخففها محلول مركز و أخففه لحين أصل
+
+180
+00:13:46,060 --> 00:13:52,240
+التركيز الفعال للفحص طبيعي أو ممكن يكون عبارة عن
+
+181
+00:13:52,240 --> 00:13:56,580
+ready to use reagent، reagent محضر و جاهز و بس
+
+182
+00:13:56,580 --> 00:14:01,440
+عليا أنا أستخدمهبالنسبة للنوع الأخر من المحاليل هي
+
+183
+00:14:01,440 --> 00:14:07,640
+ال staining solution stain .. stain معناها يصبغ
+
+184
+00:14:07,640 --> 00:14:12,500
+تمام؟ staining solution محاليل الصباغة staining
+
+185
+00:14:12,500 --> 00:14:16,420
+solution هي عبارة عن solutions that contain
+
+186
+00:14:16,420 --> 00:14:22,230
+colored dyes فيهاصبغات ملونة تمام محاليل فيها
+
+187
+00:14:22,230 --> 00:14:27,070
+صبغات ملونة طبعا هذه المحاليل ممكن تكون مشحونة
+
+188
+00:14:27,070 --> 00:14:30,370
+وممكن تكون غير مشحونة ايش يعني مشحونة وغير مشحونة
+
+189
+00:14:30,370 --> 00:14:34,490
+يعني ممكن تكون عبارة عن basic يعني شحنتها موجبة
+
+190
+00:14:34,490 --> 00:14:38,910
+ممكن تكون عبارة عن تركيبتها acidic يعني هي حمض
+
+191
+00:14:38,910 --> 00:14:42,570
+يعني تركيبتها حمضية فبالتالي لها negative charge
+
+192
+00:14:42,570 --> 00:14:46,130
+او ممكن تكون neutral يعني لا basic ولا acidic
+
+193
+00:14:46,130 --> 00:14:50,820
+وبالتالي شحنتها صفرتمام؟ فحسب طب .. طب أنا إيش
+
+194
+00:14:50,820 --> 00:14:57,080
+بستفيد من هاي المعلومة؟ لإنه أنا هاي الصبغة بصبغ
+
+195
+00:14:57,080 --> 00:15:01,320
+فيها البكتيريا، خلايا البكتيريا و الفطريات و بصبغ
+
+196
+00:15:01,320 --> 00:15:06,580
+فيها التفايليات و خلايا الدم و خلايا جسم الإنسان
+
+197
+00:15:06,580 --> 00:15:10,960
+فبالتالي لازم إنه طبعا هذه الخلايا أنا مابقدرش
+
+198
+00:15:10,960 --> 00:15:14,140
+أشوفها إلا تحت ال microscope إلا أصبغها لازم
+
+199
+00:15:14,140 --> 00:15:19,580
+تمصبغتها علشان أقدر أشوفهاطيب هذه الجزيق .. طبعا
+
+200
+00:15:19,580 --> 00:15:23,440
+جزيقات الخلايا هذه مشحونة احنا عندنا في خلاياتنا
+
+201
+00:15:23,440 --> 00:15:27,260
+بروتينات موجبة الشحنة وبروتينات سالبة الشحنة ال
+
+202
+00:15:27,260 --> 00:15:32,700
+DNA اش اسمه ال DNA؟ ديوكسي رايبون نيوكلييك أسد
+
+203
+00:15:32,700 --> 00:15:37,100
+مثلا صح؟ ديوكسي رايبون نيوكلييك أسد من اسمه acid
+
+204
+00:15:37,100 --> 00:15:43,740
+تمام؟ يعني هو شحنته سالبةفأكيد بحتاج صبغة موجبة
+
+205
+00:15:43,740 --> 00:15:47,860
+عشان ترتبط معاه و هي تصبغه عشان هيك احنا لازم تكون
+
+206
+00:15:47,860 --> 00:15:54,100
+المحاليل اما مشحونة موجبة يعني تركبتها basic فبتها
+
+207
+00:15:54,100 --> 00:15:58,300
+تصبغلي الأشياء السالبة في الخلية تمام او تكون
+
+208
+00:15:58,300 --> 00:16:03,140
+acidic و تصبغلي الأشياء الموجبة في الخلية او تكون
+
+209
+00:16:03,140 --> 00:16:06,960
+neutral يعني غير مشحونة و شحنتها صفر و بتصبغلي
+
+210
+00:16:06,960 --> 00:16:12,140
+الأشياء الغير مشحونة داخل الخلية تمام؟طيب
+
+211
+00:16:12,140 --> 00:16:15,660
+different stain are used in medical laboratories
+
+212
+00:16:15,660 --> 00:16:19,760
+في عندي أنواع كتير من الصبغات و أنواع يعني متنوعة
+
+213
+00:16:19,760 --> 00:16:24,080
+من الصبغات اللي بتستخدم في المختبرات الطبية طبعا
+
+214
+00:16:24,080 --> 00:16:28,120
+من شأن هاي ال stain الصبغاتانها تعطيني artificial
+
+215
+00:16:28,120 --> 00:16:32,660
+color لون مصطنع يعني هو مش لون الخلية الأصلي لون
+
+216
+00:16:32,660 --> 00:16:36,940
+مصطنع لحتى تمكنني من ان انا اش اعمل اقدر اعمل
+
+217
+00:16:36,940 --> 00:16:42,360
+identification تمام بتخلي الخلايا او المواد المراد
+
+218
+00:16:42,360 --> 00:16:46,680
+رؤيتها مثلا تحت ال microscope او في العينة to be
+
+219
+00:16:46,680 --> 00:16:50,620
+identified علشان انا اقدر اميزها فبنكسبها لون
+
+220
+00:16:50,620 --> 00:16:54,580
+وطبعا يمكن صباغة العينات اللي هي عبارة عن ال blood
+
+221
+00:16:55,100 --> 00:16:59,100
+دم أو body fluid سواء الجسم ال urine عشان نشوف إيش
+
+222
+00:16:59,100 --> 00:17:02,840
+فيها مايكروبات أو بكتيريا أو تفايليات أو فطريات أو
+
+223
+00:17:02,840 --> 00:17:07,700
+خلايا حتى تمام؟ إذا النوع الآخر هو ال staining
+
+224
+00:17:07,700 --> 00:17:10,580
+solution تمام؟ إيش بدي أعرف عن ال staining
+
+225
+00:17:10,580 --> 00:17:14,100
+solution؟ أول حاجة بدي أعرف إن هو مكون من colored
+
+226
+00:17:14,100 --> 00:17:18,990
+dye من سبغات ملونة تمام؟ممكن يكون شحنته موجبة يعني
+
+227
+00:17:18,990 --> 00:17:23,470
+تركيبه basic او تكون شحنته لهذا المحلول يعني ال
+
+228
+00:17:23,470 --> 00:17:27,250
+standing solution تكون شحنته سالبة تمام يعني هو
+
+229
+00:17:27,250 --> 00:17:30,730
+تركيبه acidic او تكون شحنته صفر يعني هو neutral
+
+230
+00:17:30,730 --> 00:17:34,710
+متعادل لا هو basic ولا acidic طب انا ايش بستفيد من
+
+231
+00:17:34,710 --> 00:17:39,390
+ان انا اعرف شحنة ال solution بستخدمها الاستخدام
+
+232
+00:17:39,390 --> 00:17:43,230
+الصحيح يعني انا لو بدى اصبغ نوات الخلية نوات
+
+233
+00:17:43,230 --> 00:17:48,930
+الخلية مليانة أحمض نوويةما ليها نقاش أحماض نووية
+
+234
+00:17:48,930 --> 00:17:56,550
+DNA وRNA فطبعا بما أنها هي فيها أحماض نووية لازم
+
+235
+00:17:56,550 --> 00:18:00,430
+أن أنا أصبغها بال basic solution اللي هي المحليل
+
+236
+00:18:00,430 --> 00:18:05,110
+القاعديةطبعا هي بده تكون شحنتها سالبة لإنها أحمض
+
+237
+00:18:05,110 --> 00:18:11,570
+تمام و الصبغة هتكون شحنتها موجبة لإنها basic فموجب
+
+238
+00:18:11,570 --> 00:18:15,830
+مع سالب يتم التجاذب و الالتقاء و بالتالي الصبغة
+
+239
+00:18:15,830 --> 00:18:21,210
+راح ترتبط بجزء اللي مراد صباغته و بالتالي ممكن
+
+240
+00:18:21,210 --> 00:18:25,490
+بتمكنني من رؤيته تحت ال microscope فبالتالي هذا
+
+241
+00:18:25,490 --> 00:18:29,370
+اللي بستفيده من معرفة الشحنة و حكينا إنه أنا في
+
+242
+00:18:29,370 --> 00:18:32,990
+عندي أنواع كتيرمن الصبغات اللى تستخدم في ال
+
+243
+00:18:32,990 --> 00:18:36,430
+medical laboratories اللى بتعطى المواد المراد
+
+244
+00:18:36,430 --> 00:18:39,210
+رؤيتها او الأجزاء المراد رؤيتها تحت ال microscope
+
+245
+00:18:39,210 --> 00:18:43,370
+بتعطيها artificial color لون إصطناعى طبعا انا ممكن
+
+246
+00:18:43,370 --> 00:18:49,050
+أصبغ عينات الدم سواء الجسم ال urine الخلايا ال كل
+
+247
+00:18:49,050 --> 00:18:55,490
+إشي يعني مسحات البكتيريا أي نوع يعني عينة clinical
+
+248
+00:18:55,490 --> 00:18:59,310
+sample يعني عينة تم أخدها من مريض ممكن سبغتها
+
+249
+00:18:59,310 --> 00:19:03,930
+لرؤيتهاأو يعني اللي فحصها تحت ال microscope طيب
+
+250
+00:19:03,930 --> 00:19:08,810
+نوع التالت من المحاليل هو عبارة عن ال standard
+
+251
+00:19:08,810 --> 00:19:12,710
+solutions إيش يعني standard solution؟ طبعا
+
+252
+00:19:12,710 --> 00:19:17,130
+standard solution يعني محاليل معيارية إيش يعني
+
+253
+00:19:17,130 --> 00:19:21,210
+معيارية؟ يعني أنا بعاير ب .. ب .. ب .. يعني
+
+254
+00:19:21,210 --> 00:19:26,430
+باستخدام هذا المحلول كيف بعاير يعني؟يعني مثلا انا
+
+255
+00:19:26,430 --> 00:19:34,570
+العينة تبعتي هي فيها تركيز مجهولة تمام؟ و ال
+
+256
+00:19:34,570 --> 00:19:41,090
+standard solution بيكون تركيز معلوم تمام؟ طيب فال
+
+257
+00:19:41,090 --> 00:19:46,790
+.. التركيز هاي المعلومة انا بستخدمها يعني علشان
+
+258
+00:19:46,790 --> 00:19:52,260
+اعرف التركيز داخل العينةيعني انا بستخدم محاليل
+
+259
+00:19:52,260 --> 00:19:58,140
+معلومة التركيز علشان احصل علاقة التركيز المجهولة
+
+260
+00:19:58,140 --> 00:20:02,260
+داخل العينة اذا انا بجيب التركيز داخل العينة
+
+261
+00:20:02,260 --> 00:20:07,420
+بدلالة معلوم اللى وهو ال standard solution طيب كيف
+
+262
+00:20:07,420 --> 00:20:11,600
+بيصير هذا ال issue لنفرض مثلا خليني .. لنفرض ان
+
+263
+00:20:11,600 --> 00:20:18,660
+عندي محلول standard solution تمام وعينة مريضلو انا
+
+264
+00:20:18,660 --> 00:20:22,880
+اضفت عليهم ال reagent المادة المتفاعلة مثلا عشان
+
+265
+00:20:22,880 --> 00:20:29,120
+تتفاعل معله glucose وتعطيني لون، تمام؟ طيب فعليا
+
+266
+00:20:29,120 --> 00:20:33,780
+.. فعلياال standard solution أنا عارفة تركيزه هو
+
+267
+00:20:33,780 --> 00:20:36,980
+دايما بيكون معلوم التركيز لو نفرض انه تركيزه ..
+
+268
+00:20:36,980 --> 00:20:43,140
+تركيز السكر داخل المحلول هذا مية تمام و أنا ضفت
+
+269
+00:20:43,140 --> 00:20:48,100
+لهم reagent و أعطاني لون pink نفرض فلو أعطى في
+
+270
+00:20:48,100 --> 00:20:54,220
+العينة نص شدة اللون تمام نص شدة اللون اللي موجودة
+
+271
+00:20:54,220 --> 00:20:58,460
+في ال standard راح أعرف انه تركيز العينة تبعتي
+
+272
+00:20:58,460 --> 00:21:05,030
+خمسينطبعا لإن المعلوم مية لو أعطف العينة ضعف شدة
+
+273
+00:21:05,030 --> 00:21:11,970
+اللون عن ال standard رح أعرف إنه في العينة تبعتي
+
+274
+00:21:11,970 --> 00:21:18,570
+يعني تركيز متين ضعف طبعا إذا أنا في عندي جهاز
+
+275
+00:21:18,570 --> 00:21:22,350
+بيقيص الشدة اللون اللي بيظهر من التفاعل و بيقارن
+
+276
+00:21:22,350 --> 00:21:28,320
+ليها باستخدام ال standard solution و بالتاليبيقارن
+
+277
+00:21:28,320 --> 00:21:31,720
+ليها باستخدام standard solution وبالتالي بيحسبلي
+
+278
+00:21:31,720 --> 00:21:35,960
+التركيز المجهولة داخل العينة بدلالة معلوم تمام؟
+
+279
+00:21:35,960 --> 00:21:39,880
+طيب they are used to determine the value of an
+
+280
+00:21:39,880 --> 00:21:42,760
+identical chemical with unknown concentration of a
+
+281
+00:21:42,760 --> 00:21:46,200
+given solutionيعني احنا حكينا ان هاي المحاليل
+
+282
+00:21:46,200 --> 00:21:51,180
+تستخدم على حتى نعرف ايش to determine the value of
+
+283
+00:21:51,180 --> 00:21:54,060
+an identical chemical with a non-concentration
+
+284
+00:21:54,060 --> 00:21:59,420
+يعني انا بدي اعرف كمية تركيز مادة معينة مجهولة في
+
+285
+00:21:59,420 --> 00:22:03,500
+المحلول او في العينة تبعتيطيب ال .. ال .. ال
+
+286
+00:22:03,500 --> 00:22:06,580
+chemicals that are used to prepare these solutions
+
+287
+00:22:06,580 --> 00:22:10,280
+should be of analytical grade لازم المحاليل
+
+288
+00:22:10,280 --> 00:22:13,660
+المستخدمة في تحضير ال standard solution أو المواد
+
+289
+00:22:13,660 --> 00:22:17,660
+الكيميائية المستخدمة في تحضير ال standard solution
+
+290
+00:22:17,660 --> 00:22:20,780
+تكون of analytical grade إيش يعني analytical
+
+291
+00:22:20,780 --> 00:22:26,020
+grade؟ يعني على درجة عالية من الثباتوالدقة و
+
+292
+00:22:26,020 --> 00:22:31,800
+النقاء لحتى تستخدم اتمكنني ان انا استخدمها في فحص
+
+293
+00:22:31,800 --> 00:22:36,960
+طب و اجيب تركيز العينة المجهول بدلالة هذا المعلوم
+
+294
+00:22:36,960 --> 00:22:43,660
+تمام؟ طيبSince poor standard solution cause error
+
+295
+00:22:43,660 --> 00:22:46,960
+on the estimation of the intended substance, their
+
+296
+00:22:46,960 --> 00:22:52,640
+accuracy preparation is of poor amount تمام؟ إيش
+
+297
+00:22:52,640 --> 00:22:56,000
+يعني الكلام هذا؟ يعني بيحكيلك إنه إذا كان ال
+
+298
+00:22:56,000 --> 00:23:00,160
+standard solution تم تحضيره أو تخزينه أو التعامل
+
+299
+00:23:00,160 --> 00:23:05,400
+معاه بطريقة سيئة بحيث صار تالف تمام؟هذا راح يسببلي
+
+300
+00:23:05,400 --> 00:23:11,340
+errors أخطاء وخلل في عملية تقدير أو إيجاد تركيز
+
+301
+00:23:11,340 --> 00:23:15,920
+المواد المجهولة داخل العينة تمام importance in
+
+302
+00:23:15,920 --> 00:23:18,760
+order to obtain accurate and precise laboratory
+
+303
+00:23:18,760 --> 00:23:22,740
+finding in the medical laboratory إذن أهمية
+
+304
+00:23:22,740 --> 00:23:28,020
+standard solution تنبع من إنها هي بتمكننيمن الحصول
+
+305
+00:23:28,020 --> 00:23:33,220
+على accurate and precise laboratory findings يعني
+
+306
+00:23:33,220 --> 00:23:39,540
+نتائج تمام laboratory result نتائج دقيقة وصحيحة
+
+307
+00:23:39,540 --> 00:23:42,100
+accurate and precise laboratory finding أو
+
+308
+00:23:42,100 --> 00:23:46,160
+laboratory result يعني من خلال استخدام ال standard
+
+309
+00:23:46,160 --> 00:23:50,220
+solutionممكن أنا أصنف هذا ال standard solution إلى
+
+310
+00:23:50,220 --> 00:23:54,160
+نوعين أول نوع هو ال primary standard solution
+
+311
+00:23:54,160 --> 00:23:58,460
+والنوع التاني هو ال secondary standard solution
+
+312
+00:23:58,460 --> 00:24:01,820
+طيب خلينا نشوف إيش يعني primary standard solution
+
+313
+00:24:02,120 --> 00:24:05,680
+ال primary standard solution هو عبارة عن chemical
+
+314
+00:24:05,680 --> 00:24:10,140
+solution that has the highest purity and can be
+
+315
+00:24:10,140 --> 00:24:12,820
+used directly for the exact measurement of
+
+316
+00:24:12,820 --> 00:24:15,840
+substance of unknown concentration in a given
+
+317
+00:24:15,840 --> 00:24:20,000
+solution بحكيلك أنه ال primary standard solution
+
+318
+00:24:20,000 --> 00:24:24,080
+هو عبارة عن محلول كيميائي with the highest purity
+
+319
+00:24:24,080 --> 00:24:30,720
+يعني أعلى درجات النقاء والدقة يعني الفعلية تبعته
+
+320
+00:24:31,330 --> 00:24:37,850
+فبالتالي مباشرة بقدر انا استخدمه في إيجاد تركيز
+
+321
+00:24:37,850 --> 00:24:41,790
+مجهولة باستخدام المعلوم يعني بدلالة معلوم اللي هو
+
+322
+00:24:41,790 --> 00:24:45,990
+ال primary standard solution من أمثلة هذه المحاليل
+
+323
+00:24:45,990 --> 00:24:50,010
+ال sodium chloride ال sodium bicarbonate و ال
+
+324
+00:24:50,010 --> 00:24:55,710
+potassium iodide تمام؟ طيباش لازم يعني او كيف ال
+
+325
+00:24:55,710 --> 00:24:58,210
+.. اش الخصائص اللي لازم تتوفر في ال primary
+
+326
+00:24:58,210 --> 00:25:03,230
+standard solution بحكيلك لازم ان هو يكون مصنوع من
+
+327
+00:25:03,230 --> 00:25:07,370
+مواد اول حاجة free of impurities يعني خالية من
+
+328
+00:25:07,370 --> 00:25:13,210
+الشوائب لازم يكون highest pure يعني نقي جداهذا أول
+
+329
+00:25:13,210 --> 00:25:18,890
+خاصية أو أول صفة لازم يعني تتوفر في المواد المكونة
+
+330
+00:25:18,890 --> 00:25:21,650
+لل primary standard او المكونات لل primary
+
+331
+00:25:21,650 --> 00:25:26,050
+standard تاني شغلة stable on keeping in solid
+
+332
+00:25:26,050 --> 00:25:30,530
+state and in solution يعني لازم يكون هذا المحلول
+
+333
+00:25:30,530 --> 00:25:33,950
+أو المواد المكونة لل primary أو المصنعة أو اللي من
+
+334
+00:25:33,950 --> 00:25:38,730
+خلالها انا ب ..بكون او ب .. بصنع primary standard
+
+335
+00:25:38,730 --> 00:25:43,930
+solution لازم تكون هذه المواد ثابتة فيه سواء كانت
+
+336
+00:25:43,930 --> 00:25:47,450
+في الحالة الصلبة او السائلة كيف يعني؟ يعني لو كانت
+
+337
+00:25:47,450 --> 00:25:51,470
+لساتها بودر و انا لسه محضرتش منها primary standard
+
+338
+00:25:51,470 --> 00:25:55,990
+solution طبعا لازم تظل هذه المادة تكون ثابتة لو
+
+339
+00:25:55,990 --> 00:25:59,090
+انا بدأ أحطها في درجة الحرارة حرارة الغرفة او في
+
+340
+00:25:59,090 --> 00:26:04,300
+تلاجةأو في درجة حرارة التخزين و أيضا لو أنا حضرت
+
+341
+00:26:04,300 --> 00:26:09,380
+منها محلول لازم المحلول يظل ثابت التركيز طيب إيش
+
+342
+00:26:09,380 --> 00:26:13,640
+يعني مثلا لو أنا حطيت مادة اللي هى powder بتنتص
+
+343
+00:26:13,640 --> 00:26:19,430
+رطوبةفي درجة حرارة الغرفة فبالتالي هذه المادة اللي
+
+344
+00:26:19,430 --> 00:26:24,490
+بتنتص رطوبة رح يتغير مكوناتها فبالتالي هي لاتصلح
+
+345
+00:26:24,490 --> 00:26:27,830
+لإنها تكون مكون من مكونات ال primary standard
+
+346
+00:26:27,830 --> 00:26:32,370
+solution تمام هذا معناه انه يكون stable in keeping
+
+347
+00:26:32,370 --> 00:26:35,670
+in solid state او in solution لو انا حضرت محلول
+
+348
+00:26:35,670 --> 00:26:39,270
+على أساس ان هو primary standard solution واتفاجأت
+
+349
+00:26:39,270 --> 00:26:43,680
+انه بيصير له evaporation بيتبخرمعناها هذا اشماله
+
+350
+00:26:43,680 --> 00:26:47,960
+لا يصلح انه يكون primary standard solution هاي
+
+351
+00:26:47,960 --> 00:26:52,970
+معناه انه يكون stable ثابتطبعا .. طبعا لازم كمان
+
+352
+00:26:52,970 --> 00:26:56,330
+يكون able to be accurately weighed or measured
+
+353
+00:26:56,330 --> 00:27:01,330
+لازم طبعا انا اكون جادرة ان انا اقيس اوزنه او
+
+354
+00:27:01,330 --> 00:27:06,630
+اقيسه او اعرف تركيزه بشكل دقيق جدا لانه انا تركيزه
+
+355
+00:27:06,630 --> 00:27:12,610
+هذا المعلوم تمام بدي استخدمه عشان اجي .. اجد
+
+356
+00:27:12,610 --> 00:27:18,720
+بدلقته تركيز مجهول موجود داخل العينةطيب لازم ايضا
+
+357
+00:27:18,720 --> 00:27:22,880
+يكون not hygroscopic اش يعني hygroscopic يعني يمتس
+
+358
+00:27:22,880 --> 00:27:27,200
+رتوبة hygroscopic معناها يمتس رتوبة not
+
+359
+00:27:27,200 --> 00:27:32,340
+hygroscopic يعني لا يمتس رتوبة هذا شرط من شروط ال
+
+360
+00:27:32,340 --> 00:27:35,760
+primary standard solution doesn't absorb moisture
+
+361
+00:27:35,760 --> 00:27:40,360
+and vaporize حتى كمان مابيتبخرش على درجة حرارة
+
+362
+00:27:40,360 --> 00:27:44,600
+الغرفة او حتى درجة حرارة عشرينهذا يعني الصبات
+
+363
+00:27:44,600 --> 00:27:49,880
+الصبات وإنه يكون pure هذا الخصائص اللي لازم تتوفر
+
+364
+00:27:49,880 --> 00:27:53,420
+في ال primary standard solution هو عبارة عن ال
+
+365
+00:27:53,420 --> 00:27:58,740
+highest pure solution اللي أنا من خلاله بجيب تركيز
+
+366
+00:27:58,740 --> 00:28:02,840
+مجهول داخل العينة بدلال التركيز معلوم على و هو ال
+
+367
+00:28:02,840 --> 00:28:07,240
+primary standard solution بمشي زي و زي العينة بضيف
+
+368
+00:28:07,240 --> 00:28:12,300
+عليهم الاتنين reagent و براقب التغيرلو صار تغير في
+
+369
+00:28:12,300 --> 00:28:15,160
+العينة ضعف التغير اللي في ال .. في ال standard
+
+370
+00:28:15,160 --> 00:28:18,040
+معناها التركيز في العينة ضعف التركيز اللي في ال
+
+371
+00:28:18,040 --> 00:28:24,740
+standard لو صار تغير في العينة تلت التركيز اللي ..
+
+372
+00:28:24,740 --> 00:28:27,880
+تلت التغير اللي صار في ال standard معناها التركيز
+
+373
+00:28:27,880 --> 00:28:33,820
+في العينة تلت التركيز في ال standard و هكذا تمام؟
+
+374
+00:28:34,900 --> 00:28:38,660
+طيب secondary standard solution ال secondary
+
+375
+00:28:38,660 --> 00:28:44,460
+standard solution هي عبارة عن محليل أقل نقاء lower
+
+376
+00:28:44,460 --> 00:28:50,400
+impurity تمام و إذا ان هي مابتستخدم directly علشان
+
+377
+00:28:50,400 --> 00:28:55,440
+انا اجيس فيها او استخدمها كمعلوم و اجيب يعني
+
+378
+00:28:55,440 --> 00:29:00,620
+بدلالتها تركيز مجهول لأ طبعا هي .. انا كمان
+
+379
+00:29:00,620 --> 00:29:04,460
+مابكونش عارفة تركيز تبعها بجيبهان انا بقارنها ب
+
+380
+00:29:04,460 --> 00:29:09,500
+primary standard تمام؟ اذا هو محلول مش كتير بيوت و
+
+381
+00:29:09,500 --> 00:29:14,920
+مش معلوم تركيزه بقارنه ب .. بمحالي ال primary
+
+382
+00:29:14,920 --> 00:29:19,000
+standard بمحالي ال .. ال .. ال هي يعني ال number
+
+383
+00:29:19,000 --> 00:29:23,240
+one معلومة التركيز و بعد هيك ممكن انا استخدمها بال
+
+384
+00:29:23,240 --> 00:29:26,500
+analytical procedures زي في التجارب التحليلية و
+
+385
+00:29:26,500 --> 00:29:31,970
+المعايرة و الأشياء هايطيب من الأمثلة على هذه
+
+386
+00:29:31,970 --> 00:29:35,230
+المحاليين ال nitric acid ال hydrochloric acid و ال
+
+387
+00:29:35,230 --> 00:29:40,890
+sulphuric acid طبعا إيش .. و أنا بحضر secondary
+
+388
+00:29:40,890 --> 00:29:45,980
+standard solution يعني في اعتبارات يجب أنتراهاأول
+
+389
+00:29:45,980 --> 00:29:49,400
+حاجة بدى انا و انا بحضره لازم استخدم analytical
+
+390
+00:29:49,400 --> 00:29:54,320
+balance ل .. ل .. لأخذ الأوزان يعني لو قاللي هذه
+
+391
+00:29:54,320 --> 00:29:58,720
+المادة ال powder خدي منها خمسة جرام و داوبيهم مثلا
+
+392
+00:29:58,720 --> 00:30:02,520
+بميت أمقل distilled water عشان تحصلي على secondary
+
+393
+00:30:02,520 --> 00:30:06,420
+standard solution لازم انا عشان اوزن الخمسة جرام
+
+394
+00:30:06,420 --> 00:30:11,650
+هدول استخدم analytical balanceالميزان اللي من نوع
+
+395
+00:30:11,650 --> 00:30:16,970
+analytical اش يعني يعني ميزان حساس و دقيق جدا تمام
+
+396
+00:30:16,970 --> 00:30:20,810
+طيب dissolving the weighted substance in the
+
+397
+00:30:20,810 --> 00:30:24,610
+prescribed volume الخمسة جرام هدول حكولي او
+
+398
+00:30:24,610 --> 00:30:29,150
+التعليمات اللي تحكيلي ان انا ادوبهم ب 100 ml من ال
+
+399
+00:30:29,150 --> 00:30:33,110
+solvent خلاص مية يعني مية مابنفعش 110 ولا 120
+
+400
+00:30:33,110 --> 00:30:38,160
+نهائيا انا مابنفع ألعبي الحجومdetermining the
+
+401
+00:30:38,160 --> 00:30:41,420
+exact concentration by converging against a
+
+402
+00:30:41,420 --> 00:30:45,980
+primary solution بعد ما أكون حضرت وزنتي الوزنة
+
+403
+00:30:45,980 --> 00:30:50,480
+وضفت ال exact volume of solvent من ال secondary
+
+404
+00:30:50,480 --> 00:30:56,580
+standard solution لازم أروح أنا أجيب تركيزه يعني
+
+405
+00:30:56,580 --> 00:31:01,440
+أعمله زي كأنه مجهول التركيز و أجيب تركيزه بدلالة
+
+406
+00:31:01,440 --> 00:31:04,560
+المعلوم مين هو المعلوم عندي ال primary standard
+
+407
+00:31:04,560 --> 00:31:08,310
+اللي هو رقم one في ال solutionال highest purity و
+
+408
+00:31:08,310 --> 00:31:14,890
+ال .. ال accurate concentration له طبعا و بعد هيك
+
+409
+00:31:14,890 --> 00:31:20,510
+ممكن أنا أستخدمه إما ك .. يعني ك stock عندي يعني
+
+410
+00:31:20,510 --> 00:31:25,970
+محلول مركز أصير أعمل منه dilution يعني حين
+
+411
+00:31:25,970 --> 00:31:31,130
+الاستخدام أو إن أنا أحضره جاهز ready to use طبعا
+
+412
+00:31:31,750 --> 00:31:37,190
+فإذا إيش يعني stock يعني محلولي مركز تمام كمخزون
+
+413
+00:31:37,190 --> 00:31:42,490
+عندى مركز تمام و لحين الاستخدام أنا ب .. ب .. بحضر
+
+414
+00:31:42,490 --> 00:31:45,970
+التخفيف اللى بديه او التركيز اللى بلزمني فى الشغل
+
+415
+00:31:45,970 --> 00:31:52,460
+هى معناهاطيب النوع ال .. ال .. الرابع و الأخير من
+
+416
+00:31:52,460 --> 00:31:57,620
+المحاليل اللي تستخدم في المختبرات الطبية هي ال
+
+417
+00:31:57,620 --> 00:32:01,260
+buffer solution إيش يعني buffer؟ ال buffer هو
+
+418
+00:32:01,260 --> 00:32:07,240
+المحلول الذي يقاوم التغير في ال pHتمام ال buffer
+
+419
+00:32:07,240 --> 00:32:13,020
+is a solution that resist change in pH وغالبا مكون
+
+420
+00:32:13,020 --> 00:32:18,380
+مكون من weak acid مثلا و strong base او strong
+
+421
+00:32:18,380 --> 00:32:23,420
+acid و weak base تمام اذا اما weak acid و strong
+
+422
+00:32:23,420 --> 00:32:28,370
+base لازم يكون واحد فيهم قوي و واحد ضعيف تمامweak
+
+423
+00:32:28,370 --> 00:32:32,790
+acid with strong base او strong acid with weak
+
+424
+00:32:32,790 --> 00:32:37,730
+base وفي يعني .. يعني كتب بتعبر عن هالكلام هذا
+
+425
+00:32:37,730 --> 00:32:44,210
+باستخدام مصطلح salt مصطلح salt اش يعني؟ يعني مثلا
+
+426
+00:32:44,210 --> 00:32:50,950
+ال weak acid زي ال .. ال ..ال acidic acid طبعا ال
+
+427
+00:32:50,950 --> 00:32:57,410
+acidic acid weak acid هذا طيب ايش ال .. ال .. ال
+
+428
+00:32:57,410 --> 00:33:01,750
+base طبعه اللي هو عبارة عن ال acetate طبعا و هذه
+
+429
+00:33:01,750 --> 00:33:06,790
+strong base تعتبر فاما بقول عنها ال conjugate base
+
+430
+00:33:06,790 --> 00:33:10,690
+طبعا يعني ال acid و ال base طبعته او ال acid و ال
+
+431
+00:33:10,690 --> 00:33:14,670
+salt طبعته بس المهم اللي يبديكم تعرفوه ان ال
+
+432
+00:33:14,670 --> 00:33:18,780
+buffer يستخدم من weak acid او strongbase أو strong
+
+433
+00:33:18,780 --> 00:33:24,420
+acid weaknessتمام؟ و هو من خصائصه انه يقاوم التغير
+
+434
+00:33:24,420 --> 00:33:28,020
+بالبيئة resisted change in the pH طبعا ال buffer
+
+435
+00:33:28,020 --> 00:33:35,220
+يستخدم لما انا بدي .. لما بيكون ال pH يعني مهم جدا
+
+436
+00:33:35,220 --> 00:33:40,640
+انه يكون مضبوط في التفاؤلات تبعتي او في الفحوصات
+
+437
+00:33:40,640 --> 00:33:44,960
+تبعتي يعني مثلا لو انا بستخدم او بدي اقيس تركيز
+
+438
+00:33:44,960 --> 00:33:51,950
+انزيمات او نشاط انزيمات يعني في عينة معينةيعني
+
+439
+00:33:51,950 --> 00:33:55,570
+مثلا لو أنا بدي أجيس وظائف الكبد وظائف الكبد إيش
+
+440
+00:33:55,570 --> 00:33:58,850
+يعني وظائف الكبد؟ كتير بتسمع وظائف كبد ووظائف كلة
+
+441
+00:33:58,850 --> 00:34:02,230
+طب ما هي دي أصلا وظائف كبد ووظائف كلة هي عبارة عن
+
+442
+00:34:02,230 --> 00:34:05,930
+بحوصات أنا بجيس فيها نشاط الإنزيمات اللي موجودة
+
+443
+00:34:05,930 --> 00:34:10,050
+داخل الكبد أو موجودة داخل الكلة وبتمكن الكلة من
+
+444
+00:34:10,050 --> 00:34:13,770
+إنها تشتغل شغلها أو الكبد إنه يشتغل شغله عشان هيك
+
+445
+00:34:13,770 --> 00:34:17,910
+لو كانت الإنزيمات هذه نشاط طبعها أو ال .. ال .. ال
+
+446
+00:34:17,910 --> 00:34:22,820
+activity طبعتهافعال تمام بحكي انه وظائف الكبد
+
+447
+00:34:22,820 --> 00:34:27,320
+ممتازة و وظائف الكلا ممتازة تمام؟ طيب هذه
+
+448
+00:34:27,320 --> 00:34:30,320
+الانزيمات طبعا احنا بنعرف انه اي انزيم بال ..
+
+449
+00:34:30,320 --> 00:34:34,760
+بالدنيا لازم انه يشتغل عن درجة حرارة مناسبة و pH
+
+450
+00:34:34,760 --> 00:34:40,300
+مناسب و مايكونش فيه الو inhibitor ولا يعني معيق
+
+451
+00:34:40,300 --> 00:34:46,700
+لشغله تمام؟طيب فإذا لازم انا عشان أجيس نشاط
+
+452
+00:34:46,700 --> 00:34:52,240
+الانزيمات هذه أوفر ال pH المناسب ودرجة الحرارة
+
+453
+00:34:52,240 --> 00:34:56,060
+المناسبة لإنه الانزيم يكون فعال عشان أجيس نشاطه
+
+454
+00:34:56,060 --> 00:35:00,540
+فبالتالي بستخدم buffers علشان أوفر ال pH المناسب
+
+455
+00:35:00,540 --> 00:35:04,960
+لنشاط هذا ال enzymeفبالتالي يكون الفحص طبيعي شغال
+
+456
+00:35:04,960 --> 00:35:09,800
+أو الفحص طبيعي دقيق أو معبر عن نشاط الانزيم، بنفعش
+
+457
+00:35:09,800 --> 00:35:13,440
+.. يعني أنا مثلا في عندى في جسمى انزيمات الماعدة
+
+458
+00:35:13,440 --> 00:35:18,740
+بتشتغل عند ال .. ال .. ال .. البيئات ال acidic،
+
+459
+00:35:18,740 --> 00:35:23,510
+تمام؟و عندي انزيمات ال intestine اللي هي الأمعاء
+
+460
+00:35:23,510 --> 00:35:28,670
+بتشتغل عند ال pH ال alkaline ما بنفع انا اروح اخد
+
+461
+00:35:28,670 --> 00:35:35,990
+اقيس تركيز انزيمات المعدة و انا حاطة ال pH للتفاعل
+
+462
+00:35:35,990 --> 00:35:39,850
+9 او 10 طب ما المعدة اصلا ال pH تبعها 3 و
+
+463
+00:35:39,850 --> 00:35:44,610
+الانزيمات مصممة انها تشتغل على pH 3 ليش انا اقيسها
+
+464
+00:35:44,610 --> 00:35:49,730
+في تفاعل ال pH تبعه 8 او 9؟ غلطساعتها الانزيمات مش
+
+465
+00:35:49,730 --> 00:35:53,090
+هتشتغل و انا .. و انا افكر ان الانزيمات مضروبة
+
+466
+00:35:53,090 --> 00:35:56,930
+يعني في مشكلة في المعي ده فبالتالي ابدا اعطي نتائج
+
+467
+00:35:56,930 --> 00:36:02,250
+خاطئة و تفسير خاطئ و diagnosis خاطئ فمن .. يفضل ان
+
+468
+00:36:02,250 --> 00:36:08,230
+انا اوفر ال pH المناسب لنشاط ال enzyme علشان انا
+
+469
+00:36:08,230 --> 00:36:14,010
+لما اعبر عن ال activity تبعته اعبر بشكل دقيقتمام و
+
+470
+00:36:14,010 --> 00:36:18,270
+كيف انا بوفر ال pH المناسب لهذا ال enzyme باستخدام
+
+471
+00:36:18,270 --> 00:36:24,510
+ال buffer تمام باستخدام ال bufferطيب المحاليل هذه
+
+472
+00:36:24,510 --> 00:36:29,950
+طبعا ال .. ال .. احنا حكينا يعني انه قصة التركيز
+
+473
+00:36:29,950 --> 00:36:34,650
+او نقطة التركيز لهذه المحاليل مهمة جدا، طبعا فإذا
+
+474
+00:36:34,650 --> 00:36:38,970
+لازم انا يكون طبعا انه انا اعبر عن محاليل او تركيز
+
+475
+00:36:38,970 --> 00:36:44,130
+المحاليل بشكل accurate، طبعا و باستخدام ال .. ال
+
+476
+00:36:44,130 --> 00:36:48,180
+.. ال unit المناسبةيعني لما انا بدي احكي تركيز
+
+477
+00:36:48,180 --> 00:36:54,680
+المحلول هذا خمسة طب خمسة إيش؟ ما بعرف خمسة وخلاص
+
+478
+00:36:54,680 --> 00:36:59,180
+أسكت ما .. هذا تركيز غير معبر لأ لما لكن أحكي أنا
+
+479
+00:36:59,180 --> 00:37:05,100
+خمسة milligram per deciliter أو خمسة في المية
+
+480
+00:37:05,100 --> 00:37:11,080
+weight volume أو خمسة molar إذا انا هيك افهمت إيش
+
+481
+00:37:11,080 --> 00:37:16,950
+يعني تركيزتمام؟ مجرد ما أنا ذكرت ال .. الوحدة،
+
+482
+00:37:16,950 --> 00:37:21,370
+وحدة القياس، إذا أنا عرفت أو عبرت عن التركيز بشكل
+
+483
+00:37:21,370 --> 00:37:26,520
+كتير أفضل من ما أني أعبر عن القيمة فقطو اذا لازم
+
+484
+00:37:26,520 --> 00:37:30,200
+انا اعبر او يعني يكون ال concentration دقيق و اعبر
+
+485
+00:37:30,200 --> 00:37:34,620
+عنه بالوحدة المناسبة لإله طبعا في عندي نوعين من
+
+486
+00:37:34,620 --> 00:37:38,620
+الوحدات اللي تستخدم في التعبير عن القياس في وحدات
+
+487
+00:37:38,620 --> 00:37:41,820
+بسميها physical unit ال physical unit ايش يعني
+
+488
+00:37:41,820 --> 00:37:46,060
+physical unit؟لمّا أحكي أنه وحدات فيزيائية، إيش
+
+489
+00:37:46,060 --> 00:37:49,960
+يعني؟ يعني مثلا باستخدام الوزن والطول وال ..
+
+490
+00:37:49,960 --> 00:37:55,060
+والحجم هك .. هيك يعني باعبر فيزيائيا، تمام؟
+
+491
+00:37:55,060 --> 00:37:58,820
+الفيزيائيا يعني أنه مثلا هي أخدته يمكن بال .. بال
+
+492
+00:37:58,820 --> 00:38:04,080
+.. بالفيزيا أنه يعني بأول محاضرة أن كانت اللي هي
+
+493
+00:38:04,080 --> 00:38:09,140
+عن ال .. ال .. الوحدات المعياريةال .. ال .. الوزن
+
+494
+00:38:09,140 --> 00:38:15,020
+و الطول و الحجم ال .. المسافة هي الطول اه يعني زي
+
+495
+00:38:15,020 --> 00:38:20,260
+هيك فإذا انا باستخدام هاي الوحدات بعبر عنها فإذا
+
+496
+00:38:20,260 --> 00:38:22,920
+انا باستخدم بكون physical unit للتعبير عن ال
+
+497
+00:38:22,920 --> 00:38:28,400
+concentrationأول وحدة أو اول مثال هنستخدمه اللي هو
+
+498
+00:38:28,400 --> 00:38:32,260
+ال weight to volume ال weight to volume هو عبارة
+
+499
+00:38:32,260 --> 00:38:36,920
+عن ايش يعني؟ وزن ال solute بالنسبة لحجم ال
+
+500
+00:38:36,920 --> 00:38:41,400
+solvent، تمام؟ اذا وزن ال solute بالنسبة لحجم ال
+
+501
+00:38:41,400 --> 00:38:45,300
+solvent هادي بسميها weight to volume unitفي عندي
+
+502
+00:38:45,300 --> 00:38:49,620
+محلول مثلا هيماعطينيا بيحكيلي أنه تركيز محلول ل
+
+503
+00:38:49,620 --> 00:38:54,400
+glucose solution هو عبارة عن أربعين جرام في المية
+
+504
+00:38:54,400 --> 00:38:58,240
+weight to volume أربعين جرام في المية weight to
+
+505
+00:38:58,240 --> 00:39:02,300
+volume هذي لو أنا بدي أفسرها إيش يعني؟ يعني مثلا
+
+506
+00:39:02,300 --> 00:39:08,580
+أربعين جرام من ال weight تمام؟ أربعين جرام من ال
+
+507
+00:39:08,580 --> 00:39:15,040
+weight لمين؟ لل glucoseفي ايش؟ في ميت أمقل المية
+
+508
+00:39:15,040 --> 00:39:18,680
+بالمية هذه معناها مية في ميت أمقل من ال volume
+
+509
+00:39:18,680 --> 00:39:23,600
+لمين؟ لال solvent طبعا؟ اذا كانت ال .. ال .. لو
+
+510
+00:39:23,600 --> 00:39:26,800
+انا بدي احكي اربعين جرام في المية weight و volume
+
+511
+00:39:26,800 --> 00:39:30,720
+glucose solution ايش معناه الكلام هذا؟ معناه انه
+
+512
+00:39:30,720 --> 00:39:35,980
+في محلول glucose طبعا؟ محلول سكر تم تحضيره بذابة
+
+513
+00:39:35,980 --> 00:39:39,080
+اربعين جرام ال weight هذا اللي هي وزن ال .. ال
+
+514
+00:39:39,080 --> 00:39:43,580
+solute اربعين جراممن ال glucose ال powder طبعا
+
+515
+00:39:43,580 --> 00:39:49,900
+اضافتها او اذابتها في ايش؟ في مية طبعا volume يعني
+
+516
+00:39:49,900 --> 00:39:57,120
+في ميت ml منين؟ من ال ال solvent طبعا و هنا بستخدم
+
+517
+00:39:57,120 --> 00:40:00,700
+ايش؟ ال physical unit ال physical unit ل ال weight
+
+518
+00:40:00,700 --> 00:40:06,180
+ل ال weight جرام و ال physical unit ل ال volume ml
+
+519
+00:40:06,180 --> 00:40:07,300
+طبعا
+
+520
+00:40:11,300 --> 00:40:15,020
+طيب النوع الأخر لما أحكي weight to weight طبعا
+
+521
+00:40:15,020 --> 00:40:17,960
+weight .. weight to weight أو weight per weight
+
+522
+00:40:17,960 --> 00:40:23,220
+طيب إيش يعني .. يعني وزن السوليوت بالنسبة لوزن ال
+
+523
+00:40:23,220 --> 00:40:27,440
+solvent يعني مثلا هي هنا مثال معطيني تلاتين جرام
+
+524
+00:40:27,440 --> 00:40:30,560
+في المية weight to weight hydrochloric acid إيش
+
+525
+00:40:30,560 --> 00:40:34,680
+معناها؟ معناها إنه تم تحضير هذا المحلول باستخدام
+
+526
+00:40:34,680 --> 00:40:40,140
+تلاتين جرام من ال hydrochloric acid طبعا هذا ال ..
+
+527
+00:40:40,140 --> 00:40:50,370
+السوليوتباش كملنا للمية هنا كملنا للمية يعني حطيت
+
+528
+00:40:50,370 --> 00:40:58,120
+سبعين gram من ال solventطبعا فها ده بيفرج عن ال
+
+529
+00:40:58,120 --> 00:41:01,560
+weight to volume ال weight to volume ال 40 في 100
+
+530
+00:41:01,560 --> 00:41:06,140
+طبعا أما اللي .. اللي لما .. اللي لما تكون weight
+
+531
+00:41:06,140 --> 00:41:10,320
+to weight بتكون يعني تلاتين في المية معناها تلاتين
+
+532
+00:41:10,320 --> 00:41:15,840
+في سبعين هذا الفرق ما بينهم و ال volume per volume
+
+533
+00:41:15,840 --> 00:41:21,610
+او ال volume to volume ratioمعناها هنا حجم السوليط
+
+534
+00:41:21,610 --> 00:41:26,670
+بالنسبة لحجم ال solvent تسعين في المية volume to
+
+535
+00:41:26,670 --> 00:41:30,490
+volume إيثانول إيش معناها؟ معناها أنا حضرت إيثانول
+
+536
+00:41:30,490 --> 00:41:37,280
+solution حطيت تسعين جرام من الإيثانول علىعشرة امال
+
+537
+00:41:37,280 --> 00:41:41,620
+من ال .. ال solvent أو ال distilled water تمام؟
+
+538
+00:41:41,620 --> 00:41:45,780
+اذا لما بتكون الوحدات ال ratio weight to weight
+
+539
+00:41:45,780 --> 00:41:49,780
+تمام؟ او volume to volume لما يكون هذا ال ratio
+
+540
+00:41:49,780 --> 00:41:54,580
+متجانس volume to volume هي متجانس حجم لحجم وزن
+
+541
+00:41:54,580 --> 00:42:00,280
+لوزن تمام؟ بيتم التحضير عن طريق التكميل يعني انا
+
+542
+00:42:00,280 --> 00:42:06,400
+بحط تلاتينو بكمل للمية، تمام؟ بكمل .. يعني سبعين،
+
+543
+00:42:06,400 --> 00:42:10,680
+مابحطش مش تلاتين و مية، لأ، تلاتين و سبعين، بكمل،
+
+544
+00:42:10,680 --> 00:42:14,960
+تكمل ال volume to volume نفس الاشي، متجانس هيه،
+
+545
+00:42:14,960 --> 00:42:19,420
+حطيت تسعين وكملت للمية، ضفت عشرة فقط لغير لكن لما
+
+546
+00:42:19,420 --> 00:42:25,270
+يكون weight to volume، لأما بكملش هنا وات اربعين
+
+547
+00:42:25,270 --> 00:42:29,330
+جرام و بحط المية .. المية في المية هذه يعني مية
+
+548
+00:42:29,330 --> 00:42:33,210
+أمقال distilled water ما بكملش طبعا انا .. طبعا
+
+549
+00:42:33,210 --> 00:42:36,730
+هنا الاربعين جرام انا مش عارفة ان هم .. هم ماجدش
+
+550
+00:42:36,730 --> 00:42:41,720
+حجمهم عشان اكمل تكميل مابنفعش اكملتمام فبالتالي
+
+551
+00:42:41,720 --> 00:42:45,300
+الاربعين جرام بحط عليهم ال volume زي ما هو مية في
+
+552
+00:42:45,300 --> 00:42:49,120
+المية لكن لما تكون الوحدة متجانسة بقى هستخدم اللي
+
+553
+00:42:49,120 --> 00:42:54,540
+هي التكميل يعني ان انا ب .. ال final volume الوزن
+
+554
+00:42:54,540 --> 00:42:58,480
+النهائي في النهائية بده يكون في عندي ميت جرام
+
+555
+00:42:58,480 --> 00:43:03,860
+تلاتين وسبعين الوزن النهائي مية هنا تسعين وعشرة ال
+
+556
+00:43:03,860 --> 00:43:08,790
+final volume بده يكون مية تمام؟هي معنى ال ..
+
+557
+00:43:08,790 --> 00:43:12,930
+الوحدات الفيزيائية طبعا في عندي وحدات كيميائية
+
+558
+00:43:12,930 --> 00:43:17,370
+اللي هي تستخدم في التركيز المحلي اللي .. اللي
+
+559
+00:43:17,370 --> 00:43:22,310
+تستخدم في ال .. المختبرات زي ال acid و ال bases و
+
+560
+00:43:22,310 --> 00:43:26,770
+ال reagents و أحيانا ال standards و الأشياء هذه
+
+561
+00:43:26,770 --> 00:43:32,710
+طبعاأحيانا بنعبر عنها بال specific gravity أو ال
+
+562
+00:43:32,710 --> 00:43:36,630
+percentage by weight طبعا أو أحيانا بنعبر بال ..
+
+563
+00:43:36,630 --> 00:43:41,630
+بال .. ال moral .. ال morality و ال normality طبعا
+
+564
+00:43:41,630 --> 00:43:45,970
+ال morality و ال normality طبعا طيب ال molar
+
+565
+00:43:45,970 --> 00:43:51,620
+solutionالـ molar solution أو المحلول اللي انا بده
+
+566
+00:43:51,620 --> 00:43:57,000
+اعبر عنه بالـ molarity تمام؟ اخدنا قانون مهم جدا
+
+567
+00:43:57,000 --> 00:44:00,340
+اللي هو عبارة عن او أخدتوه انتوا بالتحليلية مار
+
+568
+00:44:00,340 --> 00:44:05,160
+عليكم اللي هو عبارة عن ال molarity تساوي ال number
+
+569
+00:44:05,160 --> 00:44:09,940
+of moles على ال volume per literتمام ال number of
+
+570
+00:44:09,940 --> 00:44:14,320
+moles على ال volume per liter هذا بسميها ايش بجيس
+
+571
+00:44:14,320 --> 00:44:18,860
+انا ال molarity تبعت المحلول ال normality تمام
+
+572
+00:44:18,860 --> 00:44:24,780
+solution اللي هو عبارة عن ال ال ال molecular
+
+573
+00:44:24,780 --> 00:44:29,830
+weight على ال equivalent number على ال nتمام؟ و
+
+574
+00:44:29,830 --> 00:44:34,230
+هذه أيضا اللي هي .. اللي بسميها ال Valency تمام؟
+
+575
+00:44:34,230 --> 00:44:38,810
+بسميها ال Valency فهي الفرق ما بين ال molarity و
+
+576
+00:44:38,810 --> 00:44:48,090
+ال normality طبعا هرح أصورلكم محاضرة و أنا بكتب و
+
+577
+00:44:48,090 --> 00:44:52,550
+بتركلكم يعني و ب .. ب .. أضربلكم أمتلة على حساب ال
+
+578
+00:44:52,550 --> 00:44:58,610
+molarity و حساب ال normality تمام؟و راح توضح
+
+579
+00:44:58,610 --> 00:45:01,230
+الفكرة أكتر و راح أكتب معاها القوانين و تكون
+
+580
+00:45:01,230 --> 00:45:05,370
+المحاضرة مصورة عندكم و موجودة كمرجع فإن شاء الله
+
+581
+00:45:05,370 --> 00:45:11,070
+مش راح تتصعبوا منها مش زي ذكر المعلومة هيك و نمر
+
+582
+00:45:11,070 --> 00:45:13,730
+عنها مرور الكرام، لأ راح يكون إن شاء الله الأشي
+
+583
+00:45:13,970 --> 00:45:18,350
+ملموس عندكم من خلال ان انا راح أصور محاضرة تصوير و
+
+584
+00:45:18,350 --> 00:45:24,910
+انا بكتب و بعطيكم أمثلة و أشياء حسابية لحتى انعمق
+
+585
+00:45:24,910 --> 00:45:30,090
+فاهم إيش يعني molarity و إيش يعني normality، تمام؟
+
+586
+00:45:30,090 --> 00:45:34,330
+طيب ال .. ال dilution of solution تخفي في المحاليل
+
+587
+00:45:35,510 --> 00:45:41,790
+أيضا الجزئية هاي راح ناخد النظر منها و جزئية
+
+588
+00:45:41,790 --> 00:45:50,310
+التطبيق اللي هي الحسابات أيضا راح نتركها للمحاضرة
+
+589
+00:45:50,310 --> 00:45:54,370
+اللي .. اللي راح أصورها تصوير بحيث أنه يكون كل شيء
+
+590
+00:45:54,370 --> 00:45:58,700
+واضح ان شاء الله بالنسبة للكمخلينا بس بالأول نشوف
+
+591
+00:45:58,700 --> 00:46:02,980
+إيش يعني dilution of solution dilution يعني تخفيف
+
+592
+00:46:02,980 --> 00:46:07,620
+المحاليل تخفيف المحاليل تتذكروا أول المحاضرة إيش
+
+593
+00:46:07,620 --> 00:46:12,400
+أنا حكيتلكم معلومة حكيتلكم إن المحاليل ممكن تجيني
+
+594
+00:46:12,400 --> 00:46:16,480
+ready to use جاهزة للاستخدام ممكن تجيني powder و
+
+595
+00:46:16,480 --> 00:46:19,700
+أنا بحلها يعني بستخدم .. بتكون solute و بديف
+
+596
+00:46:19,700 --> 00:46:24,680
+solvent تمام و ممكن تجيني concentrated تمام و أنا
+
+597
+00:46:24,680 --> 00:46:29,510
+بدنا نعملي لها dilutionتكون مركزة و انا بحضر منها
+
+598
+00:46:29,510 --> 00:46:34,370
+تخفيف او تركيز معين بحيث انه يكون هو اللي يتم
+
+599
+00:46:34,370 --> 00:46:39,430
+استخدامه و اعتماده في التجربة فإذا ال dilution هو
+
+600
+00:46:39,430 --> 00:46:45,790
+عبارة عن تخفيف التركيز او تقليل التركيز لمحلول
+
+601
+00:46:45,790 --> 00:46:51,710
+معين تمام مثال تجربة عندك في البيتلو جبتي كاسة مي
+
+602
+00:46:51,710 --> 00:46:59,550
+و حطيتي فيها معلقة سكر معلقة صغيرة سكر و حطيتي نص
+
+603
+00:46:59,550 --> 00:47:05,630
+الكاسة عبيتيها ميه ضوبت السكر منيح و تذوقتي الحلقه
+
+604
+00:47:05,630 --> 00:47:11,570
+تبعته هيكون حلو مضاق حلو تمام؟طيب لنفرض أنه إنتي
+
+605
+00:47:11,570 --> 00:47:17,590
+كملتي الكاسة للآخر و دوبتي منيح عملتي إذابة منيح و
+
+606
+00:47:17,590 --> 00:47:23,210
+تذوقتي منه إيش راح نلاحظ؟ راح نلاحظ إنه مذاق الحلا
+
+607
+00:47:23,210 --> 00:47:30,010
+صار أقل أخف ليش؟ لإنه تركيز السكر صار أقل تمام؟ و
+
+608
+00:47:30,010 --> 00:47:34,410
+هذا اللي بيشرح تعريف ال dilution ال dilution هو
+
+609
+00:47:34,410 --> 00:47:40,630
+عملية تمام؟العملية اللى من خلالها ال concentration
+
+610
+00:47:40,630 --> 00:47:45,530
+تركيز او ال activity او النشاط لمحلول معين بيتم
+
+611
+00:47:45,530 --> 00:47:51,790
+ايش خفضه او تقليله تمام بيتم فيها خفض او تقليل
+
+612
+00:47:51,790 --> 00:47:56,350
+تمام ال concentration تركيز او نشاط محلول معين من
+
+613
+00:47:56,350 --> 00:48:02,230
+خلال ان انا ايش اعمل ات ضفة solventأنا قللت تركيز
+
+614
+00:48:02,230 --> 00:48:08,010
+السكر تمام قللت مستوى الحلاء تبع السكر من خلال أن
+
+615
+00:48:08,010 --> 00:48:11,410
+أنا ضفت إيش المزيد من الماء كملت الكاسة لأخرها
+
+616
+00:48:11,410 --> 00:48:17,790
+فإذا أنا ضفت المزيد من ال solvent تمامو بالتالي
+
+617
+00:48:17,790 --> 00:48:22,770
+أنا هيك قللت من تركيز السكر تمام؟ الكاسة اللي كانت
+
+618
+00:48:22,770 --> 00:48:26,950
+ممتلقة لحد نصها هي مركزة أكتر من الكاسة الممتلقة
+
+619
+00:48:26,950 --> 00:48:32,370
+كاملة فبالتالي ال dilution التخفيف يعني أن أنا
+
+620
+00:48:32,370 --> 00:48:38,190
+أضيف solvent أكتر فبالتالي أقل ال concentration ال
+
+621
+00:48:38,190 --> 00:48:46,150
+dilutionهو طبعا ممكن التعبير عنه بإيش ال ratio of
+
+622
+00:48:46,150 --> 00:48:49,750
+concentrated or stuck material to the total final
+
+623
+00:48:49,750 --> 00:48:55,330
+volume of solution هو قانون عبارة عن اللي هي الحجم
+
+624
+00:48:55,330 --> 00:48:59,910
+اللي أنا أخدته من المادة المركزة على الحجم الكائن
+
+625
+00:48:59,910 --> 00:49:04,270
+نهائي بعد مضيفة ال solventو أيضا هذا القانون راح
+
+626
+00:49:04,270 --> 00:49:09,630
+نعبر عنه راح نكتب .. نكتبه في المحاضرة المصورة و
+
+627
+00:49:09,630 --> 00:49:13,850
+راح نضرب عليه أمثلة و نشوف كيف احنا ممكن نعبر عن
+
+628
+00:49:13,850 --> 00:49:18,510
+هذا ال delusion و كيف انا ممكن اخد تطبيقات و أمثلة
+
+629
+00:49:18,510 --> 00:49:22,990
+حسابية على هذا القانون و كيف ممكن انا استخدمه في
+
+630
+00:49:22,990 --> 00:49:27,030
+حل المسائل الحسابية و لو مثلا عندي تركيز معين كيف
+
+631
+00:49:27,030 --> 00:49:30,850
+انا ممكن احضر منه تركيز آخرmany of the laboratory
+
+632
+00:49:30,850 --> 00:49:35,750
+procedures involve the use of dilution طبعا كتير
+
+633
+00:49:35,750 --> 00:49:40,350
+يعني المختبرات و تجارب المختبرات و أداء داخل
+
+634
+00:49:40,350 --> 00:49:45,150
+المختبرات بيحتاج التخفيف بيحتاج إجراء تخفيف لازم
+
+635
+00:49:45,150 --> 00:49:48,910
+أخفف العين أحيانا عشان يمكن قياسها لازم أخفف ال
+
+636
+00:49:48,910 --> 00:49:53,240
+reagent عشان يصير فعال و هكذافإذا أنا محتاجة
+
+637
+00:49:53,240 --> 00:49:58,260
+التخفيف بشكل كتير كبير و طبعا اللي بيفهم التخفيف
+
+638
+00:49:58,260 --> 00:50:03,700
+هنا في مادة المقدمة بيسهل عليه في المعامل الأخرى
+
+639
+00:50:03,700 --> 00:50:09,580
+في كل المواد بعدها انه لما يتطرقوا لجزئية ال
+
+640
+00:50:09,580 --> 00:50:13,080
+dilution يكون هو فاهمهاit's important to
+
+641
+00:50:13,080 --> 00:50:15,640
+understand the concept of dilution عشان هيك لازم
+
+642
+00:50:15,640 --> 00:50:20,460
+افهم انا ايش مبدأ ال dilution علشان بعد هيك في كل
+
+643
+00:50:20,460 --> 00:50:24,920
+المختبرات اكون فاهمة انا ايش بشتغل و كيف ممكن انا
+
+644
+00:50:24,920 --> 00:50:29,000
+احضر dilution من مادة مركزة و كيف انا استخدمها
+
+645
+00:50:29,000 --> 00:50:33,900
+طبعا these dilution have to be considered as they
+
+646
+00:50:33,900 --> 00:50:38,000
+make a quantitative difference what is going on
+
+647
+00:50:38,000 --> 00:50:44,830
+طبعا هي مما انه انا بلعب بالتركيزو بخفف تركيز عينة
+
+648
+00:50:44,830 --> 00:50:49,830
+معينة فبالتالي انا بعمل ايش؟ تغيير quantitative
+
+649
+00:50:49,830 --> 00:50:56,090
+تغيير كم على العينة طبعا ال dilution is made to
+
+650
+00:50:56,090 --> 00:51:00,470
+prepare بستخدم هذا ال dilution عشان لو كان في عندى
+
+651
+00:51:00,470 --> 00:51:05,030
+stockقبل شوية ذكرنا إيش يعني stock تمام لو كان
+
+652
+00:51:05,030 --> 00:51:08,770
+عندي stock solution مادة مركزة أحضر منها working
+
+653
+00:51:08,770 --> 00:51:14,810
+solution يعني أحضر منها تخفيف جاهز للاستخدام أو ل
+
+654
+00:51:14,810 --> 00:51:17,470
+measurable concentration of sample يعني مثلا
+
+655
+00:51:17,470 --> 00:51:22,270
+أحيانا بتكون العينة تركيز السكر فيها خمسمية يعني
+
+656
+00:51:22,270 --> 00:51:27,430
+كتير عالي و ال kit عندي أو المادة المتفاعلة بتجيس
+
+657
+00:51:27,430 --> 00:51:33,740
+لحد يعني أقصها لحد تلاتمية طب أنا إيش أعمل؟المادة
+
+658
+00:51:33,740 --> 00:51:36,840
+المتفاعلة مش هتجيس للنسبة للسكر لإن السكر في
+
+659
+00:51:36,840 --> 00:51:41,520
+العينة أكتر مش هتكون النتيجة معبرة عن العينة فانا
+
+660
+00:51:41,520 --> 00:51:46,340
+عشان أعمل أروح أخف في العينة تمام علشان أنزل السكر
+
+661
+00:51:46,340 --> 00:51:51,660
+اللي فيها وبالتالي أقدر أنا أجيسها باستخدام المادة
+
+662
+00:51:51,660 --> 00:51:54,950
+المتفاعلة اللي موجودة عندىلأن المادة المتفاعلة
+
+663
+00:51:54,950 --> 00:51:59,830
+السعة تبعتها او ال capacity تبعتها او قدرتها انها
+
+664
+00:51:59,830 --> 00:52:04,010
+تكشف ل 300 و العينة فيها 500 ايش الحل اخفف العينة
+
+665
+00:52:04,010 --> 00:52:08,990
+تمام؟ عشان تصير العينة اشمالها measurable يمكن
+
+666
+00:52:08,990 --> 00:52:14,530
+قياسهاطيب في عندي نوعين من ال .. من ال dilution
+
+667
+00:52:14,530 --> 00:52:20,510
+اللي هو واحد اسمه simple dilution تمام؟ و اللي هو
+
+668
+00:52:20,510 --> 00:52:24,790
+يعني مجرد مثلا انا اخد من مادة مركزة حجم معين و
+
+669
+00:52:24,790 --> 00:52:28,450
+اضيف عليه حجم اخر من ال solvent و هيك انا عملت
+
+670
+00:52:28,450 --> 00:52:32,580
+simple dilution تمام؟أما ال serial dilution هو
+
+671
+00:52:32,580 --> 00:52:36,840
+عبارة عن multiple و progressive dilution يعني مثلا
+
+672
+00:52:36,840 --> 00:52:40,220
+أنا كان عندي مادة مركزة أخدت منها حجم وضفت solvent
+
+673
+00:52:40,220 --> 00:52:44,500
+في أنبوبة رقم واحد بعدين أخدت من أنبوبة رقم واحد
+
+674
+00:52:44,500 --> 00:52:48,300
+حجم معين وحطته في تيوبة رقم اتنين وضفت عليها
+
+675
+00:52:48,300 --> 00:52:53,660
+solvent بعدين أخدت حجم من تيوبة رقم اتنين وضفته في
+
+676
+00:52:53,660 --> 00:52:56,880
+تيوبة رقم تلاتة وضفت عليها solvent فانا اش ما
+
+677
+00:52:56,880 --> 00:53:02,340
+عامالي بخفف أكتر و أكتر و أكترمن ال .. المادة
+
+678
+00:53:02,340 --> 00:53:07,160
+المراد عمل تخفيف لإلها و من استخدام ايش؟
+
+679
+00:53:07,160 --> 00:53:10,080
+بالاستخدام multiple dilutions انا استخدمت tu برقم
+
+680
+00:53:10,080 --> 00:53:13,400
+واحد بعد tu برقم اتنين بعد tu برقم تلاتة و هكذا
+
+681
+00:53:13,400 --> 00:53:17,080
+فبالتالي انا حصلت على multiple dilutions ففي
+
+682
+00:53:17,080 --> 00:53:21,500
+النهاية ال .. هذا بسميه serial dilution اللي هو
+
+683
+00:53:21,500 --> 00:53:25,880
+التخفيف المتسلسل من tu برقم واحد لاتنين لتلاتة و
+
+684
+00:53:25,880 --> 00:53:33,710
+هكذاطيب و أيضا ممكن انا استخدمه علشان انا لو كان ب
+
+685
+00:53:33,710 --> 00:53:38,190
+.. لو كان عندي .. بدي محاليل كتير تركيزها قليل او
+
+686
+00:53:38,190 --> 00:53:41,970
+علشان انا لو بدي احصل على محاليل ب .. في .. يعني
+
+687
+00:53:41,970 --> 00:53:47,870
+الفروق ما بينها ثابتة في ال .. التركيز تمام؟ هو
+
+688
+00:53:47,870 --> 00:53:51,910
+عبارة عن multiple حكينا و ال total dilution هو the
+
+689
+00:53:51,910 --> 00:53:55,490
+product of each dilution in the seriesهو ال total
+
+690
+00:53:55,490 --> 00:54:00,690
+dilution يعني التخفيف النهائي هو مجموع ضرب كل ال
+
+691
+00:54:00,690 --> 00:54:04,930
+dilution تبع الأنابيب كلها هنحكي إيش يعني بالظبط
+
+692
+00:54:04,930 --> 00:54:10,590
+تمام ال serial dilution بيبدأ في البداية بيكون
+
+693
+00:54:10,590 --> 00:54:14,210
+عبارة عن simple يعني أنا عندي stock تمام أخدت منه
+
+694
+00:54:14,210 --> 00:54:17,830
+حجم وحطت عليه solvent فتيوبة رقم واحد التيوبة رقم
+
+695
+00:54:17,830 --> 00:54:23,420
+واحد لحالة هاي بسميها simple dilution تمامو بعد
+
+696
+00:54:23,420 --> 00:54:27,200
+هيك التخفيفات التانية اللي في تيوب رقم اتنين و
+
+697
+00:54:27,200 --> 00:54:30,740
+تلاتة و هكذا هدول أشمع لهم بسميهم subsequent
+
+698
+00:54:30,740 --> 00:54:38,260
+dilutions ويعني تخفيفات متتابعة تيوب رقم واحد وما
+
+699
+00:54:38,260 --> 00:54:43,180
+يليها كلهم مع بعضهم بسميهم serial dilutionتمام
+
+700
+00:54:43,180 --> 00:54:48,120
+بسميهم serial dilution it has been known that if
+
+701
+00:54:48,120 --> 00:54:52,280
+we use larger volume we obtain a more accurate
+
+702
+00:54:52,280 --> 00:54:58,620
+dilution اذا كان .. اذا انا استخدمت حجوم اكتر يعني
+
+703
+00:54:58,620 --> 00:55:02,640
+حجوم اكبر بحصل على accurate dilution يعني مثلا لو
+
+704
+00:55:02,640 --> 00:55:09,960
+في النهاية لو انا بدي احصل على يعني بدي واحد لألف
+
+705
+00:55:09,960 --> 00:55:16,940
+واحد لألفdelution يعني أخد one microliter و أح ..
+
+706
+00:55:16,940 --> 00:55:22,100
+و أحطهم على تسعمية وتسعة وتسعين microliter مينفعش
+
+707
+00:55:22,100 --> 00:55:25,600
+واحد microliter يعني الحجم لا يكاد يورا بالعين
+
+708
+00:55:25,600 --> 00:55:30,930
+المجردة تمام؟فغلط طب لأ انا ليش ما اضاعف الكمية
+
+709
+00:55:30,930 --> 00:55:34,410
+اخد مثلا واحد امال بدل ال microliter و احطه في
+
+710
+00:55:34,410 --> 00:55:37,870
+تسعمية وتسعة وتسعين امال هيك بيكون احسن اذا انا
+
+711
+00:55:37,870 --> 00:55:42,410
+لازم لما بدي صح انا بكون بدي استخدم حجوم قليلة في
+
+712
+00:55:42,410 --> 00:55:46,370
+التفاعلات تبعتي لكن احضر ال dilution بكميات اكبر
+
+713
+00:55:46,370 --> 00:55:50,070
+بيكون التفاعل اضاق او يعني التركيز اللي انا بحصل
+
+714
+00:55:50,070 --> 00:55:54,090
+عليها او ال dilution اللي بحصل عليه اكثر دقةتمام
+
+715
+00:55:54,090 --> 00:55:57,190
+لما بدى اعمل performing serial dilution in the
+
+716
+00:55:57,190 --> 00:56:01,710
+clinical laboratory one must know the dilution of
+
+717
+00:56:01,710 --> 00:56:07,270
+each tube لازم اعرف التركيز لكل نبوبة داخل ال ..
+
+718
+00:56:07,270 --> 00:56:16,630
+ال ..العينة تبعتي طيب و بالتالي هذا طبعا هدول ال
+
+719
+00:56:16,630 --> 00:56:20,770
+.. هدول الأمثلة انا ان شاء الله راح أصور محاضرة و
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+720
+00:56:20,770 --> 00:56:27,230
+أنا بشرحلكم كيف انا بحصل على التركيز ل .. ل ..
+
+721
+00:56:27,230 --> 00:56:33,760
+يعنيش اسمه simple dilution و التركيز كيف انا ايضا
+
+722
+00:56:33,760 --> 00:56:37,200
+برضه بحصل عليها ل serial dilution يعني كيف انا
+
+723
+00:56:37,200 --> 00:56:40,160
+بطبق لو أجاتكم مسائل على ال primary dilution او
+
+724
+00:56:40,160 --> 00:56:42,820
+اللي هو ال simple dilution او على ال serial
+
+725
+00:56:42,820 --> 00:56:46,500
+dilution كيف انتوا تشتغلوا و هنحكي عن الأمثلة هذه
+
+726
+00:56:46,500 --> 00:56:52,710
+و نتناولها بشكل دقيق يعني أفضللمّا أكون أنا بشرح و
+
+727
+00:56:52,710 --> 00:56:56,310
+بكتب بنفس الوقت أفضل من ما اني بس أشار عليها بال
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+728
+00:56:56,310 --> 00:57:01,290
+mouse علشان يكون هيك عمقنا الفهم عندكم أكتر، لذا
+
+729
+00:57:01,290 --> 00:57:04,790
+هذه المحاضرة يتبعها محاضرة مصورة ان شاء الله ان
+
+730
+00:57:04,790 --> 00:57:10,190
+تدروها راح .. ان شاء الله راح تظهر لعندكم و
+
+731
+00:57:10,190 --> 00:57:14,330
+بالتالي يعني راح أحملكم إياها drive و بالتالي
+
+732
+00:57:14,330 --> 00:57:18,910
+تدرسوهم التنتين مع بعض علشان تتضح الصورة لديكم
+
+733
+00:57:19,510 --> 00:57:25,570
+يعطيكم العافية و ان شاء الله بنشوفكم يعني أو
+
+734
+00:57:25,570 --> 00:57:29,130
+بننزلكم المحاضرة المصورة قريبا
+
diff --git a/PL9fwy3NUQKwY9jl8xw-bWkVZkWcGGyspd/6stpniKSY70_raw.json b/PL9fwy3NUQKwY9jl8xw-bWkVZkWcGGyspd/6stpniKSY70_raw.json
new file mode 100644
index 0000000000000000000000000000000000000000..3d7e2112f297ee2d9ba767fb29d1939f28794551
--- /dev/null
+++ b/PL9fwy3NUQKwY9jl8xw-bWkVZkWcGGyspd/6stpniKSY70_raw.json
@@ -0,0 +1 @@
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"text": "هذا ال reagent لما انا بشتريه من الشركة ممكن يكون جاهز ready to use تمام ممكن يكون جاهز ready to use او ممكن يكون عبارة عن powder و انا بدي احله بدي احوله لمحلول يعني بدي اضيف عليه solvent ال solvent هذا ممكن تكون ميه distilled water تمام او اي محلول بتوصي فيه الشركة", "tokens": [3224, 15730, 2423, 26949, 317, 5296, 15042, 1975, 8315, 4724, 8592, 2655, 16572, 3224, 9154, 25124, 31747, 3660, 3714, 43020, 7251, 30544, 10874, 40294, 11622, 1919, 281, 764, 46811, 10943, 3714, 43020, 7251, 30544, 10874, 40294, 11622, 1919, 281, 764, 1975, 2407, 3714, 43020, 7251, 30544, 6225, 3555, 9640, 3660, 18871, 6341, 4032, 1975, 8315, 4724, 16254, 1975, 5016, 43761, 4724, 16254, 1975, 5016, 12610, 3224, 32767, 5016, 1211, 12610, 37495, 22653, 4724, 16254, 1975, 11242, 33911, 47356, 33575, 2423, 33575, 23758, 3714, 43020, 6055, 30544, 3714, 1829, 3224, 1483, 6261, 1281, 46811, 10943, 1975, 2407, 1975, 1829, 3714, 5016, 1211, 12610, 39894, 2407, 9381, 1829, 8978, 3224, 25124, 31747, 3660], 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"probability": 0.91650390625}, {"start": 437.05, "end": 437.73, "word": " و", "probability": 0.96337890625}, {"start": 437.73, "end": 438.91, "word": " انتباه", "probability": 0.83648681640625}, {"start": 438.91, "end": 440.11, "word": " أثناء", "probability": 0.9608154296875}, {"start": 440.11, "end": 441.29, "word": " تحضير", "probability": 0.98828125}, {"start": 441.29, "end": 441.93, "word": " هذا", "probability": 0.94091796875}, {"start": 441.93, "end": 442.19, "word": " ال", "probability": 0.89697265625}, {"start": 442.19, "end": 442.69, "word": " reagent", "probability": 0.861328125}], "temperature": 1.0}, {"id": 20, "seek": 46371, "start": 443.25, "end": 463.71, "text": "لازم يكون في عندي care اهتمام including the following should be taken in preparing a given reagent solution و انا بحضر reagent solution لازم يكون في عندي انتباه يستدعي انه انا احافظ على الأشياء التالية ايش هي ايش اللي بده احافظ عليه؟ اول حاجة ال chemical selection", "tokens": [1211, 31377, 2304, 7251, 30544, 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" احافظ", "probability": 0.9600830078125}, {"start": 461.49, "end": 462.19, "word": " عليه؟", "probability": 0.6971435546875}, {"start": 462.19, "end": 462.43, "word": " اول", "probability": 0.8447265625}, {"start": 462.43, "end": 462.75, "word": " حاجة", "probability": 0.9677734375}, {"start": 462.75, "end": 462.85, "word": " ال", "probability": 0.94140625}, {"start": 462.85, "end": 463.11, "word": " chemical", "probability": 0.95849609375}, {"start": 463.11, "end": 463.71, "word": " selection", "probability": 0.94482421875}], "temperature": 1.0}, {"id": 21, "seek": 49118, "start": 464.9, "end": 491.18, "text": "اش يعني chemical selection يعني انا اختار ال reagent الكيميائي المناسب لفحصي اذا انا بدي اقيس بروتينات مابنفع استخدم reagent بيكشف عن وجود مثلا السكر في الدم لازم يكون ايش ال reagent مختص بيه الفحص تبعي و احنا سبق و حكينا انه كل reagent بيكون مصمم لاجراء فحص معين فانا لازم اكون عندى ال selection تبع الاختيار لهذا ال reagent", "tokens": [33599, 37495, 22653, 7313, 9450, 37495, 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"end": 512.37, "text": "ختيار المادة الكيميائية المناسبة لهذا ال reagent بحيث انه اتناسب الفحص الطبيعي تمام؟ و اذا مابنفع انا يكون عندي فحص بدي اجيس مثلا مستوى الدهون في الجسم و اروح اختار اعمل selection ل reagent المادة الكيميائية اللى فيه متخصصة في الكشف عن البروتينات مش الدهون غلط", "tokens": [9778, 31371, 9640, 9673, 18513, 3660, 33251, 32640, 1829, 16373, 10632, 9673, 8315, 35457, 3660, 46740, 15730, 2423, 26949, 317, 4724, 5016, 1829, 12984, 16472, 3224, 1975, 2655, 8315, 35457, 27188, 5016, 9381, 41950, 21292, 3615, 1829, 46811, 10943, 22807, 4032, 1975, 15730, 19446, 3555, 1863, 5172, 3615, 1975, 8315, 7251, 30544, 18871, 16254, 6156, 5016, 9381, 4724, 16254, 1975, 7435, 1829, 3794, 50113, 15040, 3714, 14851, 2407, 7578, 32748, 3224, 11536, 8978, 25724, 38251, 4032, 1975, 32887, 5016, 1975, 46456, 9640, 1975, 25957, 1211, 9450, 5296, 26949, 317, 9673, 18513, 3660, 33251, 32640, 1829, 16373, 10632, 13672, 7578, 8978, 3224, 44650, 9778, 9381, 9381, 3660, 8978, 33251, 8592, 5172, 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ملاحظته بحيث ان انا بعد هيك احصل على قراءات او احصل على نتيجة طبعا و لازم انا استدعي الانتباه و الاهتمام اثناء", "tokens": [9566, 3555, 3615, 995, 23758, 20666, 1863, 35457, 6027, 1829, 2423, 5480, 3827, 1975, 15730, 13672, 1829, 4724, 16254, 1975, 3615, 28480, 3224, 18871, 2423, 5480, 3827, 16472, 31439, 6225, 3555, 9640, 3660, 18871, 9673, 18513, 3660, 9673, 2655, 5172, 995, 30241, 3660, 20449, 18863, 9957, 3660, 13672, 1829, 9154, 13412, 33456, 11296, 37279, 9566, 9957, 1829, 1319, 15844, 18863, 9957, 3660, 6055, 17082, 13546, 15844, 18863, 9957, 3660, 7251, 43020, 12174, 25528, 3794, 3224, 4032, 32640, 19452, 3714, 15040, 5016, 19913, 47395, 4724, 5016, 1829, 12984, 16472, 1975, 8315, 39182, 39896, 4117, 1975, 5016, 36520, 15844, 12174, 2288, 16606, 9307, 1975, 2407, 1975, 5016, 36520, 15844, 8717, 31371, 7435, 3660, 23032, 3555, 3615, 995, 4032, 5296, 31377, 2304, 1975, 8315, 44713, 3215, 3615, 1829, 2423, 7649, 2655, 3555, 40294, 4032, 2423, 40294, 39237, 10943, 1975, 12984, 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{"start": 760.24, "end": 761.34, "word": " ملاحظته", "probability": 0.98994140625}, {"start": 761.34, "end": 762.02, "word": " بحيث", "probability": 0.9716796875}, {"start": 762.02, "end": 762.2, "word": " ان", "probability": 0.82177734375}, {"start": 762.2, "end": 762.54, "word": " انا", "probability": 0.876220703125}, {"start": 762.54, "end": 762.86, "word": " بعد", "probability": 0.95556640625}, {"start": 762.86, "end": 763.56, "word": " هيك", "probability": 0.78515625}, {"start": 763.56, "end": 764.44, "word": " احصل", "probability": 0.9482421875}, {"start": 764.44, "end": 764.78, "word": " على", "probability": 0.8828125}, {"start": 764.78, "end": 765.52, "word": " قراءات", "probability": 0.83612060546875}, {"start": 765.52, "end": 765.68, "word": " او", "probability": 0.955810546875}, {"start": 765.68, "end": 765.98, "word": " احصل", "probability": 0.9791666666666666}, {"start": 765.98, "end": 766.18, "word": " على", "probability": 0.953125}, {"start": 766.18, "end": 766.82, "word": " نتيجة", "probability": 0.995361328125}, {"start": 766.82, "end": 767.44, "word": " طبعا", "probability": 0.88720703125}, {"start": 767.44, "end": 768.1, "word": " و", "probability": 0.88525390625}, {"start": 768.1, "end": 768.64, "word": " لازم", "probability": 0.8797200520833334}, {"start": 768.64, "end": 768.96, "word": " انا", "probability": 0.955078125}, {"start": 768.96, "end": 769.72, "word": " استدعي", "probability": 0.916015625}, {"start": 769.72, "end": 770.52, "word": " الانتباه", "probability": 0.9779296875}, {"start": 770.52, "end": 770.94, "word": " و", "probability": 0.67822265625}, {"start": 770.94, "end": 772.14, "word": " الاهتمام", "probability": 0.8814697265625}, {"start": 772.14, "end": 772.82, "word": " اثناء", "probability": 0.8919677734375}], "temperature": 1.0}, {"id": 35, "seek": 79455, "start": 772.97, "end": 794.55, "text": "اختيار نوع ال reagent يكون ملائم لنوع الفحص اللى بدنا احنا بصدد إجراءه و أيضا بدي أتبع تعليمات أثناء تحضيره تعليمات الشركة المصنية على هذا ال reagent بدي أصنعه بكميات و بأحجام دقيقة بحيث أن أنا أظل محافظة على تركيزه المناسب للفحص", "tokens": [47283, 31371, 9640, 8717, 45367, 2423, 26949, 317, 7251, 30544, 3714, 1211, 16373, 2304, 5296, 1863, 45367, 27188, 5016, 9381, 13672, 7578, 47525, 8315, 1975, 5016, 8315, 4724, 9381, 3215, 3215, 11933, 7435, 2288, 16606, 3224, 4032, 36632, 11242, 995, 4724, 16254, 5551, 2655, 3555, 3615, 37279, 20292, 2304, 9307, 5551, 12984, 1863, 16606, 6055, 5016, 11242, 13546, 3224, 37279, 20292, 2304, 9307, 25124, 31747, 3660, 9673, 9381, 1863, 10632, 15844, 23758, 2423, 26949, 317, 4724, 16254, 5551, 9381, 1863, 3615, 3224, 4724, 24793, 1829, 9307, 4032, 4724, 10721, 5016, 7435, 10943, 11778, 38436, 28671, 4724, 5016, 1829, 12984, 14739, 41850, 5551, 19913, 1211, 3714, 5016, 31845, 19913, 3660, 15844, 6055, 31747, 1829, 11622, 3224, 9673, 8315, 35457, 24976, 5172, 5016, 9381], "avg_logprob": -0.10118140098525256, "compression_ratio": 1.7296137339055795, "no_speech_prob": 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0.822265625}, {"start": 786.29, "end": 786.57, "word": " هذا", "probability": 0.88525390625}, {"start": 786.57, "end": 786.67, "word": " ال", "probability": 0.94482421875}, {"start": 786.67, "end": 787.27, "word": " reagent", "probability": 0.98486328125}, {"start": 787.27, "end": 788.01, "word": " بدي", "probability": 0.901123046875}, {"start": 788.01, "end": 789.39, "word": " أصنعه", "probability": 0.88857421875}, {"start": 789.39, "end": 790.09, "word": " بكميات", "probability": 0.9759521484375}, {"start": 790.09, "end": 790.23, "word": " و", "probability": 0.5419921875}, {"start": 790.23, "end": 790.89, "word": " بأحجام", "probability": 0.9677734375}, {"start": 790.89, "end": 791.39, "word": " دقيقة", "probability": 0.9954427083333334}, {"start": 791.39, "end": 791.69, "word": " بحيث", "probability": 0.9781494140625}, {"start": 791.69, "end": 791.83, "word": " أن", "probability": 0.50634765625}, {"start": 791.83, "end": 791.97, "word": " أنا", "probability": 0.5712890625}, {"start": 791.97, "end": 792.27, "word": " أظل", "probability": 0.7737630208333334}, {"start": 792.27, "end": 792.73, "word": " محافظة", "probability": 0.99580078125}, {"start": 792.73, "end": 792.87, "word": " على", "probability": 0.85205078125}, {"start": 792.87, "end": 793.35, "word": " تركيزه", "probability": 0.97783203125}, {"start": 793.35, "end": 793.85, "word": " المناسب", "probability": 0.9931640625}, {"start": 793.85, "end": 794.55, "word": " للفحص", "probability": 0.97412109375}], "temperature": 1.0}, {"id": 36, "seek": 81761, "start": 794.93, "end": 817.61, "text": "و أيضا أحطه في ال bottle أو ال container المناسبة لإله بحيث أني انا أضل أحافظ على فعاليته و أحافظ على خصائصه ما تتغير تمام؟ و حكينا أن هذا ال reagent بيجي من شركات كل reagent بيكون خاص بفحص معين ممكن يجيني powder عبارة عن solute و أنا بدي أضيفله solvent عشان أحوله ل solution", "tokens": [2407, 36632, 11242, 995, 5551, 5016, 9566, 3224, 8978, 2423, 7817, 34051, 2423, 10129, 9673, 8315, 35457, 3660, 5296, 28814, 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"word": " معين", "probability": 0.99365234375}, {"start": 812.21, "end": 812.81, "word": " ممكن", "probability": 0.974609375}, {"start": 812.81, "end": 813.29, "word": " يجيني", "probability": 0.9097900390625}, {"start": 813.29, "end": 813.67, "word": " powder", "probability": 0.416015625}, {"start": 813.67, "end": 814.17, "word": " عبارة", "probability": 0.9903564453125}, {"start": 814.17, "end": 814.35, "word": " عن", "probability": 0.99658203125}, {"start": 814.35, "end": 814.87, "word": " solute", "probability": 0.767822265625}, {"start": 814.87, "end": 814.97, "word": " و", "probability": 0.38916015625}, {"start": 814.97, "end": 815.07, "word": " أنا", "probability": 0.6201171875}, {"start": 815.07, "end": 815.27, "word": " بدي", "probability": 0.6923828125}, {"start": 815.27, "end": 815.71, "word": " أضيفله", "probability": 0.8319091796875}, {"start": 815.71, "end": 816.15, "word": " solvent", "probability": 0.57568359375}, {"start": 816.15, "end": 816.61, "word": " عشان", 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"temperature": 1.0}, {"id": 130, "seek": 294179, "start": 2919.83, "end": 2941.79, "text": "هو طبعا ممكن التعبير عنه بإيش ال ratio of concentrated or stuck material to the total final volume of solution هو قانون عبارة عن اللي هي الحجم اللي أنا أخدته من المادة المركزة على الحجم الكائن نهائي بعد مضيفة ال solvent", "tokens": [3224, 2407, 23032, 3555, 3615, 995, 3714, 43020, 16712, 3615, 3555, 13546, 18871, 3224, 4724, 28814, 1829, 8592, 2423, 8509, 295, 21321, 420, 5541, 2527, 281, 264, 3217, 2572, 5523, 295, 3827, 31439, 12174, 7649, 11536, 6225, 3555, 9640, 3660, 18871, 13672, 1829, 39896, 21542, 7435, 2304, 13672, 1829, 41850, 5551, 9778, 3215, 47395, 9154, 9673, 18513, 3660, 9673, 31747, 11622, 3660, 15844, 21542, 7435, 2304, 33251, 16373, 1863, 8717, 3224, 16373, 1829, 39182, 3714, 11242, 33911, 3660, 2423, 33575], "avg_logprob": -0.11168981775825407, "compression_ratio": 1.5305164319248827, "no_speech_prob": 1.7285346984863281e-06, "words": [{"start": 2919.83, "end": 2920.35, "word": "هو", "probability": 0.888916015625}, {"start": 2920.35, "end": 2920.99, "word": " طبعا", "probability": 0.981689453125}, {"start": 2920.99, "end": 2923.67, "word": " ممكن", "probability": 0.92919921875}, {"start": 2923.67, "end": 2924.23, "word": " التعبير", "probability": 0.9720458984375}, {"start": 2924.23, "end": 2924.65, "word": " عنه", "probability": 0.945556640625}, {"start": 2924.65, "end": 2925.19, "word": " بإيش", "probability": 0.6934661865234375}, {"start": 2925.19, "end": 2925.55, "word": " ال", "probability": 0.70556640625}, {"start": 2925.55, "end": 2925.95, "word": " ratio", "probability": 0.98876953125}, {"start": 2925.95, "end": 2926.15, "word": " of", "probability": 0.978515625}, {"start": 2926.15, "end": 2926.89, "word": " concentrated", "probability": 0.90478515625}, {"start": 2926.89, "end": 2927.21, "word": " or", "probability": 0.82373046875}, {"start": 2927.21, "end": 2927.61, "word": " stuck", "probability": 0.556640625}, {"start": 2927.61, "end": 2928.25, "word": " material", "probability": 0.75390625}, {"start": 2928.25, "end": 2928.67, "word": " to", "probability": 0.95556640625}, {"start": 2928.67, "end": 2928.85, "word": " the", "probability": 0.9013671875}, {"start": 2928.85, "end": 2929.17, "word": " total", "probability": 0.8251953125}, {"start": 2929.17, "end": 2929.75, "word": " final", "probability": 0.888671875}, {"start": 2929.75, "end": 2930.21, "word": " volume", "probability": 0.94775390625}, {"start": 2930.21, "end": 2930.43, "word": " of", "probability": 0.97509765625}, {"start": 2930.43, "end": 2931.03, "word": " solution", "probability": 0.92724609375}, {"start": 2931.03, "end": 2932.23, "word": " هو", "probability": 0.80517578125}, {"start": 2932.23, "end": 2933.03, "word": " قانون", "probability": 0.9944661458333334}, {"start": 2933.03, "end": 2933.81, "word": " عبارة", "probability": 0.99560546875}, {"start": 2933.81, "end": 2934.07, "word": " عن", "probability": 0.99560546875}, {"start": 2934.07, "end": 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"word": " حسابية", "probability": 0.99658203125}, {"start": 2959.15, "end": 2959.39, "word": " على", "probability": 0.88916015625}, {"start": 2959.39, "end": 2959.75, "word": " هذا", "probability": 0.98583984375}, {"start": 2959.75, "end": 2960.29, "word": " القانون", "probability": 0.9970703125}, {"start": 2960.29, "end": 2960.43, "word": " و", "probability": 0.99072265625}, {"start": 2960.43, "end": 2960.61, "word": " كيف", "probability": 0.93359375}, {"start": 2960.61, "end": 2960.95, "word": " ممكن", "probability": 0.990234375}, {"start": 2960.95, "end": 2961.31, "word": " انا", "probability": 0.91357421875}, {"start": 2961.31, "end": 2962.75, "word": " استخدمه", "probability": 0.9102783203125}, {"start": 2962.75, "end": 2962.99, "word": " في", "probability": 0.94140625}, {"start": 2962.99, "end": 2963.31, "word": " حل", "probability": 0.99169921875}, {"start": 2963.31, "end": 2963.87, "word": " المسائل", "probability": 0.8875732421875}, {"start": 2963.87, "end": 2964.77, "word": " 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هنا في مادة المقدمة بيسهل عليه في المعامل الأخرى في كل المواد بعدها انه لما يتطرقوا لجزئية ال dilution يكون هو فاهمها", "tokens": [5172, 28814, 15730, 41850, 3714, 33753, 26108, 3660, 16712, 9778, 5172, 33911, 4724, 8592, 28820, 9122, 2655, 13546, 9122, 3555, 13546, 4032, 23032, 3555, 3615, 995, 13672, 1829, 4724, 33911, 16095, 16712, 9778, 5172, 33911, 34105, 8978, 3714, 18513, 3660, 9673, 28543, 46007, 4724, 1829, 3794, 3224, 1211, 47356, 8978, 9673, 3615, 10943, 1211, 16247, 34740, 7578, 8978, 28242, 9673, 14407, 3215, 39182, 11296, 16472, 3224, 5296, 15042, 7251, 2655, 9566, 2288, 4587, 14407, 5296, 7435, 11622, 19986, 10632, 2423, 11504, 1448, 7251, 30544, 31439, 6156, 995, 16095, 11296], "avg_logprob": -0.0944444440305233, "compression_ratio": 1.6910994764397906, "no_speech_prob": 3.039836883544922e-06, "words": [{"start": 2992.04, "end": 2992.5, "word": "فإذا", "probability": 0.8990885416666666}, {"start": 2992.5, "end": 2992.72, "word": " أنا", "probability": 0.57080078125}, 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[{"start": 3411.33, "end": 3411.67, "word": "لمّا", "probability": 0.7854817708333334}, {"start": 3411.67, "end": 3411.95, "word": " أكون", "probability": 0.88330078125}, {"start": 3411.95, "end": 3412.17, "word": " أنا", "probability": 0.80322265625}, {"start": 3412.17, "end": 3412.61, "word": " بشرح", "probability": 0.974609375}, {"start": 3412.61, "end": 3412.71, "word": " و", "probability": 0.783203125}, {"start": 3412.71, "end": 3413.05, "word": " بكتب", "probability": 0.9837646484375}, {"start": 3413.05, "end": 3413.37, "word": " بنفس", "probability": 0.76708984375}, {"start": 3413.37, "end": 3413.77, "word": " الوقت", "probability": 0.9933268229166666}, {"start": 3413.77, "end": 3414.13, "word": " أفضل", "probability": 0.9677734375}, {"start": 3414.13, "end": 3414.27, "word": " من", "probability": 0.849609375}, {"start": 3414.27, "end": 3414.41, "word": " ما", "probability": 0.705078125}, {"start": 3414.41, "end": 3414.65, "word": " اني", "probability": 0.64453125}, {"start": 3414.65, "end": 3414.83, "word": " بس", "probability": 0.9716796875}, {"start": 3414.83, "end": 3415.79, "word": " أشار", "probability": 0.666259765625}, {"start": 3415.79, "end": 3416.13, "word": " عليها", "probability": 0.92919921875}, {"start": 3416.13, "end": 3416.31, "word": " بال", "probability": 0.978515625}, {"start": 3416.31, "end": 3416.69, "word": " mouse", "probability": 0.8955078125}, {"start": 3416.69, "end": 3417.73, "word": " علشان", "probability": 0.97900390625}, {"start": 3417.73, "end": 3418.31, "word": " يكون", "probability": 0.98974609375}, {"start": 3418.31, "end": 3418.75, "word": " هيك", "probability": 0.689208984375}, {"start": 3418.75, "end": 3419.27, "word": " عمقنا", "probability": 0.9384765625}, {"start": 3419.27, "end": 3419.77, "word": " الفهم", "probability": 0.94580078125}, {"start": 3419.77, "end": 3420.35, "word": " عندكم", "probability": 0.984130859375}, {"start": 3420.35, "end": 3421.11, "word": " أكتر،", "probability": 0.821630859375}, {"start": 3421.11, "end": 3421.29, "word": " لذا", "probability": 0.65185546875}, {"start": 3421.29, "end": 3421.59, "word": " هذه", "probability": 0.8388671875}, {"start": 3421.59, "end": 3422.27, "word": " المحاضرة", "probability": 0.9737548828125}, {"start": 3422.27, "end": 3423.03, "word": " يتبعها", "probability": 0.9791015625}, {"start": 3423.03, "end": 3423.55, "word": " محاضرة", "probability": 0.9886474609375}, {"start": 3423.55, "end": 3424.13, "word": " مصورة", "probability": 0.9193115234375}, {"start": 3424.13, "end": 3424.27, "word": " ان", "probability": 0.7607421875}, {"start": 3424.27, "end": 3424.43, "word": " شاء", "probability": 0.977294921875}, {"start": 3424.43, "end": 3424.59, "word": " الله", "probability": 0.9521484375}, {"start": 3424.59, "end": 3424.79, "word": " ان", "probability": 0.5859375}, {"start": 3424.79, "end": 3425.37, "word": " تدروها", "probability": 0.821044921875}, {"start": 3425.37, "end": 3426.33, "word": " راح", "probability": 0.5694580078125}, {"start": 3426.33, "end": 3426.43, "word": " ..", "probability": 0.8134765625}, {"start": 3426.43, "end": 3427.31, "word": " ان", "probability": 0.904296875}, {"start": 3427.31, "end": 3427.51, "word": " شاء", "probability": 0.99462890625}, {"start": 3427.51, "end": 3427.79, "word": " الله", "probability": 0.96044921875}, {"start": 3427.79, "end": 3428.47, "word": " راح", "probability": 0.96484375}, {"start": 3428.47, "end": 3429.09, "word": " تظهر", "probability": 0.9991455078125}, {"start": 3429.09, "end": 3429.87, "word": " لعندكم", "probability": 0.9715576171875}, {"start": 3429.87, "end": 3430.19, "word": " و", "probability": 0.7509765625}, {"start": 3430.19, "end": 3431.39, "word": " بالتالي", "probability": 0.888916015625}, {"start": 3431.39, "end": 3431.95, "word": " يعني", "probability": 0.8896484375}, {"start": 3431.95, "end": 3432.15, "word": " راح", "probability": 0.96630859375}, {"start": 3432.15, "end": 3432.67, "word": " أحملكم", "probability": 0.7667236328125}, {"start": 3432.67, "end": 3432.95, "word": " إياها", "probability": 0.8458658854166666}, {"start": 3432.95, "end": 3433.39, "word": " drive", "probability": 0.88134765625}, {"start": 3433.39, "end": 3433.71, "word": " و", "probability": 0.9462890625}, {"start": 3433.71, "end": 3434.33, "word": " بالتالي", "probability": 0.98046875}, {"start": 3434.33, "end": 3435.43, "word": " تدرسوهم", "probability": 0.8863932291666666}, {"start": 3435.43, "end": 3435.89, "word": " التنتين", "probability": 0.640869140625}, {"start": 3435.89, "end": 3436.11, "word": " مع", "probability": 0.99755859375}, {"start": 3436.11, "end": 3436.51, "word": " بعض", "probability": 0.994873046875}, {"start": 3436.51, "end": 3437.23, "word": " علشان", "probability": 0.9840494791666666}, {"start": 3437.23, "end": 3437.79, "word": " تتضح", "probability": 0.9461669921875}, {"start": 3437.79, "end": 3438.37, "word": " الصورة", "probability": 0.8489583333333334}, {"start": 3438.37, "end": 3438.91, "word": " لديكم", "probability": 0.8603515625}], "temperature": 1.0}, {"id": 152, "seek": 344913, "start": 3439.51, "end": 3449.13, "text": "يعطيكم العافية و ان شاء الله بنشوفكم يعني أو بننزلكم المحاضرة المصورة قريبا", "tokens": [40228, 9566, 1829, 24793, 18863, 31845, 10632, 4032, 16472, 13412, 16606, 21984, 44945, 8592, 38688, 24793, 37495, 22653, 34051, 44945, 1863, 11622, 23275, 2304, 9673, 5016, 46958, 25720, 9673, 9381, 13063, 3660, 12174, 16572, 3555, 995], "avg_logprob": -0.07469383768133216, "compression_ratio": 1.393939393939394, "no_speech_prob": 2.980232238769531e-07, "words": [{"start": 3439.51, "end": 3440.01, "word": "يعطيكم", "probability": 0.9390869140625}, {"start": 3440.01, "end": 3440.75, "word": " العافية", "probability": 0.9851888020833334}, {"start": 3440.75, "end": 3441.47, "word": " و", "probability": 0.9794921875}, {"start": 3441.47, "end": 3441.67, "word": " ان", "probability": 0.77099609375}, {"start": 3441.67, "end": 3441.89, "word": " شاء", "probability": 0.9873046875}, {"start": 3441.89, "end": 3442.23, "word": " الله", "probability": 0.96484375}, {"start": 3442.23, "end": 3443.75, "word": " بنشوفكم", "probability": 0.9869384765625}, {"start": 3443.75, "end": 3444.45, "word": " يعني", "probability": 0.762451171875}, {"start": 3444.45, "end": 3445.57, "word": " أو", "probability": 0.4501953125}, {"start": 3445.57, "end": 3447.57, "word": " بننزلكم", "probability": 0.9451171875}, {"start": 3447.57, "end": 3448.09, "word": " المحاضرة", "probability": 0.9561767578125}, {"start": 3448.09, "end": 3448.63, "word": " المصورة", "probability": 0.9847412109375}, {"start": 3448.63, "end": 3449.13, "word": " قريبا", "probability": 0.9925537109375}], "temperature": 1.0}], "language": "ar", "language_probability": 1.0, "duration": 3450.4155, "duration_after_vad": 3424.0906249999944}
\ No newline at end of file
diff --git a/PL9fwy3NUQKwY9jl8xw-bWkVZkWcGGyspd/6stpniKSY70_raw.srt b/PL9fwy3NUQKwY9jl8xw-bWkVZkWcGGyspd/6stpniKSY70_raw.srt
new file mode 100644
index 0000000000000000000000000000000000000000..f617d8a925e886cc574fcc522c9ea37b680060e0
--- /dev/null
+++ b/PL9fwy3NUQKwY9jl8xw-bWkVZkWcGGyspd/6stpniKSY70_raw.srt
@@ -0,0 +1,2936 @@
+1
+00:00:04,390 --> 00:00:07,410
+السلام عليكم ورحمة الله وبركاته عزيزات الطالبات
+
+2
+00:00:07,410 --> 00:00:13,150
+معكم ان شاء الله في المحاضرة نظرية رقم اتنين لمساق
+
+3
+00:00:13,150 --> 00:00:17,830
+مقدمة في التحاليل التدبية ان شاء الله اليوم راحنا
+
+4
+00:00:17,830 --> 00:00:22,790
+ناخد مختصر او نبدأ عن ال solutions المحاليل اللي
+
+5
+00:00:22,790 --> 00:00:26,810
+بتستخدم في المختبرات التدبية solutions used in
+
+6
+00:00:26,810 --> 00:00:30,590
+medical laboratory المحاليل اللي بتستخدم في
+
+7
+00:00:30,590 --> 00:00:36,270
+المختبرات التدبيةبداية بدنا نعرف إيش يعني solution
+
+8
+00:00:36,270 --> 00:00:40,090
+solution كمحلول هي عبارة عن هو عبارة عن
+
+9
+00:00:40,090 --> 00:00:43,950
+homogeneous mixture هو عبارة عن خليط متجانس من
+
+10
+00:00:43,950 --> 00:00:47,550
+مكونين أو أكثر طبعا إذا هو عبارة عن homogeneous
+
+11
+00:00:47,550 --> 00:00:52,910
+mixture of two or more substancesطبعا طيب كمحلول
+
+12
+00:00:52,910 --> 00:00:58,590
+لابد انه يحتوي او يتكون من شغلتان solute اللي هي
+
+13
+00:00:58,590 --> 00:01:03,970
+الأشياء اللي تم تدويبها في المحلول و ال solvent
+
+14
+00:01:03,970 --> 00:01:09,390
+طبعا فال soluteهو عبارة عن ال dissolved substance
+
+15
+00:01:09,390 --> 00:01:13,870
+المادة المذابة whereas solvent هو is a substance
+
+16
+00:01:13,870 --> 00:01:17,230
+in which solute dissolved to make the mixture اذا
+
+17
+00:01:17,230 --> 00:01:21,970
+المحلول هو عبارة عن خليط من مكونات او اكثر
+
+18
+00:01:21,970 --> 00:01:26,690
+والمكونات هذه ممكن تنقسم الى اما تكون solute يعني
+
+19
+00:01:26,690 --> 00:01:32,510
+اشياء تم تذوبها تمام اشياء مذابة و solvent المذيب
+
+20
+00:01:32,510 --> 00:01:39,720
+هو المادة اللى ال solute تذوب فيهتمام ففي عندي
+
+21
+00:01:39,720 --> 00:01:43,560
+أنواع .. يعني أنواع مختلفة من المحاليل اللي تستخدم
+
+22
+00:01:43,560 --> 00:01:47,060
+في ال medical laboratory و بتجارب اللي بنشريها في
+
+23
+00:01:47,060 --> 00:01:52,180
+داخل المختبر الطبي من خلال او من ضمن هذه المحاليل
+
+24
+00:01:52,180 --> 00:01:56,480
+يعني ممكن احنا نصنفها ل reagent solutionنوع من
+
+25
+00:01:56,480 --> 00:02:00,480
+أنواع المحلي اللي بسميها reagent solution نوع أخر
+
+26
+00:02:00,480 --> 00:02:04,900
+بسميه staining solution و standard solution and
+
+27
+00:02:04,900 --> 00:02:08,420
+buffer solution راح ناخد كل واحدة على حدة و نعرف
+
+28
+00:02:08,420 --> 00:02:11,240
+إيش يعني إيش يعني reagent إيش يعني staining
+
+29
+00:02:11,240 --> 00:02:15,500
+solution standard and buffer solution خلينا نبلش
+
+30
+00:02:15,500 --> 00:02:18,980
+بأول واحدة اللي هي reagent solutionsإيش يعني رأيها
+
+31
+00:02:18,980 --> 00:02:23,140
+genre solution؟ طبعا إحنا عارفين إنه بس نجري فحص
+
+32
+00:02:23,140 --> 00:02:27,360
+طبي على عينة مريض لابد إنه مثلا على فرض إن أنا بدي
+
+33
+00:02:27,360 --> 00:02:30,960
+أقيس مستوى السكر في الدم طبعا أنا بدي أقيس ال
+
+34
+00:02:30,960 --> 00:02:34,420
+glucose مستوى السكر في الدم اللي هو ال glucose طيب
+
+35
+00:02:35,150 --> 00:02:39,090
+لابد من إنه يكون في عندي مادة متفاعلة تتفاعل مع ال
+
+36
+00:02:39,090 --> 00:02:44,170
+glucose في الدم علشان في النهاية تعطيني إما لون أو
+
+37
+00:02:44,170 --> 00:02:49,330
+تفاعل يعني هيصير في change هيصير في تغير معين أنا
+
+38
+00:02:49,330 --> 00:02:53,770
+بقدر يعني أقيس هذا التغير وبالتالي هذا التغير
+
+39
+00:02:53,770 --> 00:02:58,210
+بيعبرلي عن تركيز ال glucose في الدمنرجع تاني نعيد
+
+40
+00:02:58,210 --> 00:03:02,790
+نحكي أنه على فرض أنا بدي أقيس مستوى السكر في الدم
+
+41
+00:03:02,790 --> 00:03:07,450
+لابد من وجود مادة متفاعلة تتفاعل مع السكر هذا مع
+
+42
+00:03:07,450 --> 00:03:12,110
+ال glucose و بتعطيني تغير معين التغير هذا ممكن
+
+43
+00:03:12,110 --> 00:03:16,370
+يكون تغير في اللون ممكن يكون تساعد غازات ممكن يكون
+
+44
+00:03:16,510 --> 00:03:21,410
+ممكن يكون ظهور فقاعات ممكن يكون يعني أي نوع من
+
+45
+00:03:21,410 --> 00:03:24,370
+التغير حتى يعني رائحة بس احنا مابنعتمد كتير على
+
+46
+00:03:24,370 --> 00:03:28,650
+الروائح والاشياء هذه احنا دايما التغيرات بتكون
+
+47
+00:03:28,650 --> 00:03:33,990
+تغير في اللون او حتى ظهور غاز او تساعد غازات او
+
+48
+00:03:33,990 --> 00:03:39,650
+فقاعات يعني اشياءيمكن قياسها التغير يمكن قياسه
+
+49
+00:03:39,650 --> 00:03:43,950
+تمام بمجرد تفاعل هذا ال reagent او المادة
+
+50
+00:03:43,950 --> 00:03:49,790
+المتفاعلة مع ال glucose حدوث تغير لابد ان يكون
+
+51
+00:03:49,790 --> 00:03:54,490
+التغير هذا يمكن قياسه بحيث انه يعبرلي عن مستوى
+
+52
+00:03:54,490 --> 00:03:58,630
+السكر في الدم يعني لو فرضنا ان المادة المتفاعلة
+
+53
+00:03:58,630 --> 00:04:01,590
+اللي هتتفاعل مع هذا ال glucose بتعطيني لون pink
+
+54
+00:04:02,140 --> 00:04:07,500
+طبعا لون pink فخلينا نفرض مثلا كل ما زادت شدة
+
+55
+00:04:07,500 --> 00:04:12,900
+اللون كل ما زاد ال pink طبعا هيعطين .. هي .. هيخطر
+
+56
+00:04:12,900 --> 00:04:18,880
+في دهني انه في عندي كمية كبيرة من الاتفاعل صارت
+
+57
+00:04:18,880 --> 00:04:23,480
+يعني كمية كبيرة من ال glucose اتفاعلت مع ال agent
+
+58
+00:04:23,480 --> 00:04:29,550
+و أعطتني اللون ال pink فبالتاليإذا التغير في اللون
+
+59
+00:04:29,550 --> 00:04:33,870
+هذا اللي انا قدرت أقيصه قدرت أعرف إيش شدت اللون
+
+60
+00:04:33,870 --> 00:04:39,790
+تمام عكسلي جدش في عندي تركيز في العينة تمام هذا
+
+61
+00:04:39,790 --> 00:04:43,110
+كتقديم لل reaction solution خلينا نشوف إيش مكتوب
+
+62
+00:04:43,110 --> 00:04:47,630
+عندنا بال reaction solutionهو عبارة عن أي محلول
+
+63
+00:04:47,630 --> 00:04:52,470
+يمكن يستخدمه that is used in conjunction with a
+
+64
+00:04:52,470 --> 00:04:57,770
+given sample يمكن يتم استخدامه مع عينة مع العينة
+
+65
+00:04:57,770 --> 00:05:02,490
+تمام و بالتالي expected to produce a measurable or
+
+66
+00:05:02,490 --> 00:05:06,630
+noticable change هيصير مجلد ما يتفاعل مع مكون من
+
+67
+00:05:06,630 --> 00:05:11,210
+مكونات العينة هذا ال reaction هيحدث measurable or
+
+68
+00:05:11,210 --> 00:05:16,350
+noticable changeيعني هيحدث تغير يمكن ملاحظته او
+
+69
+00:05:16,350 --> 00:05:21,810
+يمكن قياسه فهذا المحلول اللي بيحققل الشرط هذا انه
+
+70
+00:05:21,810 --> 00:05:26,370
+بيتفاعل مع مكون من مكونات العينة وبيحدث تغير
+
+71
+00:05:26,370 --> 00:05:30,770
+التغير هذا هيحدث تغير على العينة يمكن قياسه او
+
+72
+00:05:30,770 --> 00:05:35,610
+ملاحظته على طول بسمي هذا المحلول عبارة عن reagent
+
+73
+00:05:35,610 --> 00:05:40,050
+solution تمام؟ طيب وانا بشتغل طبعا ال reagent
+
+74
+00:05:40,050 --> 00:05:44,370
+solution هاي المحاليل المتفاعلةلما ب .. أنا بطلبها
+
+75
+00:05:44,370 --> 00:05:49,490
+من الشركات طبعا كل نوع فحص يلو reagent خاص فيه،
+
+76
+00:05:49,490 --> 00:05:52,910
+يعني لما بدي أقيس مستوى الدهون في الجسم ب .. ب ..
+
+77
+00:05:52,910 --> 00:05:56,150
+بستخدم reagent خاص في الكشف عن الدهون، لما بدي
+
+78
+00:05:56,150 --> 00:06:00,750
+أقيس مستوى السكر في الدم بستخدم reagent متخصص في
+
+79
+00:06:00,750 --> 00:06:05,060
+الكشف عن ال glucoseلو بدي اقيس مستوى بروتينات بدي
+
+80
+00:06:05,060 --> 00:06:08,900
+استخدم reagent متخصص في الكشف عن البروتينات نشاط
+
+81
+00:06:08,900 --> 00:06:12,320
+انزيمات بستخدم reagent متخصص في الكشف عن نشاط
+
+82
+00:06:12,320 --> 00:06:16,860
+الانزيمات و هكذا اذا هذه ال reagents انا بشتريها
+
+83
+00:06:16,860 --> 00:06:21,340
+من شركات خاصة هي بتصمملي الفحص يعني هي بتصمم هذا
+
+84
+00:06:21,340 --> 00:06:25,720
+ال reagent و بت .. بتعرف تركيبه الكيميائي و بتصممه
+
+85
+00:06:25,720 --> 00:06:30,280
+على اساس ان هو يكون متخصص لإجراء فحص طبي معين طيب
+
+86
+00:06:30,670 --> 00:06:34,830
+هذا ال reagent لما انا بشتريه من الشركة ممكن يكون
+
+87
+00:06:34,830 --> 00:06:40,050
+جاهز ready to use تمام ممكن يكون جاهز ready to use
+
+88
+00:06:40,050 --> 00:06:44,570
+او ممكن يكون عبارة عن powder و انا بدي احله بدي
+
+89
+00:06:44,570 --> 00:06:47,830
+احوله لمحلول يعني بدي اضيف عليه solvent ال solvent
+
+90
+00:06:47,830 --> 00:06:51,430
+هذا ممكن تكون ميه distilled water تمام او اي محلول
+
+91
+00:06:51,430 --> 00:06:56,570
+بتوصي فيه الشركةفإذا بيجيني powders solute بيكون
+
+92
+00:06:56,570 --> 00:07:00,370
+solute تمام و أنا بضيف عليه solvent عشان أكون
+
+93
+00:07:00,370 --> 00:07:06,130
+solution تمام أو ممكن يجيني مركز و أنا أخففه بحيث
+
+94
+00:07:06,130 --> 00:07:10,590
+أحصل على التخفيف المناسب للتجربة تبعتي أو للتحص
+
+95
+00:07:10,590 --> 00:07:17,730
+تبعي تمام أصبح في عندي necessary care اهتمام و
+
+96
+00:07:17,730 --> 00:07:24,610
+انتباه أثناء تحضير هذا ال reagentلازم يكون في عندي
+
+97
+00:07:24,610 --> 00:07:28,150
+care اهتمام including the following should be
+
+98
+00:07:28,150 --> 00:07:31,750
+taken in preparing a given reagent solution و انا
+
+99
+00:07:31,750 --> 00:07:35,830
+بحضر reagent solution لازم يكون في عندي انتباه
+
+100
+00:07:35,830 --> 00:07:40,770
+يستدعي انه انا احافظ على الأشياء التالية ايش هي
+
+101
+00:07:40,770 --> 00:07:43,110
+ايش اللي بده احافظ عليه؟ اول حاجة ال chemical
+
+102
+00:07:43,110 --> 00:07:47,740
+selectionاش يعني chemical selection يعني انا اختار
+
+103
+00:07:47,740 --> 00:07:51,400
+ال reagent الكيميائي المناسب لفحصي اذا انا بدي
+
+104
+00:07:51,400 --> 00:07:54,960
+اقيس بروتينات مابنفع استخدم reagent بيكشف عن وجود
+
+105
+00:07:54,960 --> 00:07:59,180
+مثلا السكر في الدم لازم يكون ايش ال reagent مختص
+
+106
+00:07:59,180 --> 00:08:02,900
+بيه الفحص تبعي و احنا سبق و حكينا انه كل reagent
+
+107
+00:08:02,900 --> 00:08:07,540
+بيكون مصمم لاجراء فحص معين فانا لازم اكون عندى ال
+
+108
+00:08:07,540 --> 00:08:12,390
+selection تبع الاختيار لهذا ال reagentختيار المادة
+
+109
+00:08:12,390 --> 00:08:15,790
+الكيميائية المناسبة لهذا ال reagent بحيث انه
+
+110
+00:08:15,790 --> 00:08:19,670
+اتناسب الفحص الطبيعي تمام؟ و اذا مابنفع انا يكون
+
+111
+00:08:19,670 --> 00:08:23,950
+عندي فحص بدي اجيس مثلا مستوى الدهون في الجسم و
+
+112
+00:08:23,950 --> 00:08:28,430
+اروح اختار اعمل selection ل reagent المادة
+
+113
+00:08:28,430 --> 00:08:31,190
+الكيميائية اللى فيه متخصصة في الكشف عن البروتينات
+
+114
+00:08:31,190 --> 00:08:35,750
+مش الدهون غلطساعتها الفحص الطبيعي هيكون خاطئ انا
+
+115
+00:08:35,750 --> 00:08:40,930
+مش هاخد نتيجة على مستوى الدهون هاخد نتيجة على
+
+116
+00:08:40,930 --> 00:08:46,190
+مستوى البروتينات وهذا غلط طيب الشغل التاني اللي
+
+117
+00:08:46,190 --> 00:08:49,290
+بدي أنتبهلها following instruction of preparation
+
+118
+00:08:49,290 --> 00:08:54,290
+ذاكرين لما حكينا بمحاضرة ال safety ان كل مادة
+
+119
+00:08:54,290 --> 00:09:01,950
+كيميائية بيكون عليها label بتكون معلمة label عليها
+
+120
+00:09:04,780 --> 00:09:08,880
+بالاسم والتركيبة الكيميائية وال expiration date
+
+121
+00:09:08,880 --> 00:09:13,940
+اللي هو تاريخ الانتهاء الصلاحية و .. يعني خطوات
+
+122
+00:09:13,940 --> 00:09:18,040
+أثناء تحضير هذا المحلول أو يعني تعليمات لتحضيره
+
+123
+00:09:18,040 --> 00:09:22,020
+تعليمات لاستخدامه تعليمات لمحافظة على سلامتنا
+
+124
+00:09:22,020 --> 00:09:26,160
+أثناء استخدامه ممكن يكون عليه علامات hazard
+
+125
+00:09:26,160 --> 00:09:29,940
+symbolsإشارات على هذا ال reagent أو هذه المادة
+
+126
+00:09:29,940 --> 00:09:32,680
+الكيميائية بحيث أنها تتنبهني هذا في acid، هذا
+
+127
+00:09:32,680 --> 00:09:39,500
+حارق، هذا ممكن يعملي تطير منه غازات و يسبب اختناق،
+
+128
+00:09:39,500 --> 00:09:43,560
+هذا ممكن ي .. تطلع منه إشعاعاتفهذه كلها بتكون
+
+129
+00:09:43,560 --> 00:09:47,480
+موجودة وين على ال label فأنا لازم أقرأ ال label
+
+130
+00:09:47,480 --> 00:09:55,340
+كتير منيح و أنتبه و أتبع تعليمات التحضير جيدا ليش؟
+
+131
+00:09:55,340 --> 00:10:00,020
+لإن أنا بدي أحضره بجودة عالية علشان أحصل على
+
+132
+00:10:00,020 --> 00:10:06,060
+reagentتركيزه دقيق فبالتالي يفحصلي او يشتغلي الفحص
+
+133
+00:10:06,060 --> 00:10:11,400
+بشكل دقيق عشان احصل على نتائج دقيقة وبالتالي اساهم
+
+134
+00:10:11,400 --> 00:10:16,580
+في ال accurate diagnosis و ال .. ال valuable ايش
+
+135
+00:10:16,580 --> 00:10:23,020
+treatment في التشخيص الدقيق و العلاج الفعال تمام؟
+
+136
+00:10:24,090 --> 00:10:28,050
+طيب، إذا الخطوة التالتة أو الشغل التالتة اللي أنا
+
+137
+00:10:28,050 --> 00:10:31,130
+بدي أنتبهلها أثناء تحضير ال reagent أنه أنا بدي
+
+138
+00:10:31,130 --> 00:10:36,170
+أستخدم using accurate measurement of substance،
+
+139
+00:10:36,170 --> 00:10:41,330
+تمام؟ حكينا إنه اللازم أكون منتبهة كتير على
+
+140
+00:10:41,330 --> 00:10:46,770
+الحجوم، جداش مثلا إذا طلع .. حكيلي مثلا خуди من
+
+141
+00:10:46,770 --> 00:10:54,010
+هذا المادة خمسة صنطي و اخلطيهم بلتر ميهتمام؟ بنفعش
+
+142
+00:10:54,010 --> 00:10:59,620
+أنا أخلطهم ب letter و ربع أو letter و نصغلط لازم
+
+143
+00:10:59,620 --> 00:11:02,980
+انا اعمل accurate measurement of substances لازم
+
+144
+00:11:02,980 --> 00:11:07,800
+انا يكون عندي احضره بحجوم دقيقة علشان احصل على
+
+145
+00:11:07,800 --> 00:11:12,340
+تركيز دقيق وبالتالي الشغل يكون كمان دقيق using of
+
+146
+00:11:12,340 --> 00:11:15,700
+appropriate type of glass or plasticware اذا هذا
+
+147
+00:11:15,700 --> 00:11:20,420
+المحلول انا بده احضره بعدك بده احطه في bottle لازم
+
+148
+00:11:20,420 --> 00:11:23,980
+ال bottle تكون مناسبة للحفاظ على ال reagent كده
+
+149
+00:11:23,980 --> 00:11:28,680
+يعنيفي reagentات و هيمر معاكم خلال رحلتكم في دراسة
+
+150
+00:11:28,680 --> 00:11:33,880
+التحاليل الطبية هيمر معاكم أنواع من ال reagents
+
+151
+00:11:33,880 --> 00:11:40,580
+يفضل حفظها ب dark bottle يعني هي حساسة للضوء و
+
+152
+00:11:40,580 --> 00:11:45,080
+لازم انا احطها في bottle لونها بني او أسود هيمر
+
+153
+00:11:45,080 --> 00:11:50,970
+معانا مثلا ال acid و ..و ال acid و ال .. و ال base
+
+154
+00:11:50,970 --> 00:11:55,970
+لازم أحطهم في glass container بس لازم الغطايا
+
+155
+00:11:55,970 --> 00:11:59,350
+تبعته ما تكون glass خدنا هذه المعلومة المحاضرة
+
+156
+00:11:59,350 --> 00:12:02,190
+الماضية حكينا أنه مش لازم تكون ال container ..
+
+157
+00:12:02,190 --> 00:12:07,990
+الغطايا glass علشان ما ت .. يعني تعلق و يصعب فتحه
+
+158
+00:12:08,540 --> 00:12:13,100
+تمام؟ في بعض المحليين لازم أحطها في plastic bottle
+
+159
+00:12:13,100 --> 00:12:17,540
+حسب فبالتالي نوع ال reagent هو اللي بيحكملي أو
+
+160
+00:12:17,540 --> 00:12:20,800
+تركيبته الكيميائية هي اللي بتحكملي ال bitter أو ال
+
+161
+00:12:20,800 --> 00:12:26,780
+best container لإلهطبعا هذا بالنسبالي ال reaction
+
+162
+00:12:26,780 --> 00:12:29,040
+solution اذا اللي بدي اعرفه عن ال reaction
+
+163
+00:12:29,040 --> 00:12:33,120
+solution ان هو عبارة عن المادة المتفاعلة مع العينة
+
+164
+00:12:33,120 --> 00:12:36,820
+اللي من شأنها تعطيني change على العينة تغير على
+
+165
+00:12:36,820 --> 00:12:42,860
+العينة يمكن قياسه ويمكن ملاحظته بحيث ان انا بعد
+
+166
+00:12:42,860 --> 00:12:48,640
+هيك احصل على قراءات او احصل على نتيجة طبعا و لازم
+
+167
+00:12:48,640 --> 00:12:54,470
+انا استدعي الانتباه و الاهتمام اثناءاختيار نوع ال
+
+168
+00:12:54,470 --> 00:12:59,210
+reagent يكون ملائم لنوع الفحص اللى بدنا احنا بصدد
+
+169
+00:12:59,210 --> 00:13:04,550
+إجراءه و أيضا بدي أتبع تعليمات أثناء تحضيره
+
+170
+00:13:04,550 --> 00:13:08,010
+تعليمات الشركة المصنية على هذا ال reagent بدي
+
+171
+00:13:08,010 --> 00:13:12,730
+أصنعه بكميات و بأحجام دقيقة بحيث أن أنا أظل محافظة
+
+172
+00:13:12,730 --> 00:13:17,030
+على تركيزه المناسب للفحصو أيضا أحطه في ال bottle
+
+173
+00:13:17,030 --> 00:13:20,350
+أو ال container المناسبة لإله بحيث أني انا أضل
+
+174
+00:13:20,350 --> 00:13:25,030
+أحافظ على فعاليته و أحافظ على خصائصه ما تتغير
+
+175
+00:13:25,030 --> 00:13:29,790
+تمام؟ و حكينا أن هذا ال reagent بيجي من شركات كل
+
+176
+00:13:29,790 --> 00:13:33,670
+reagent بيكون خاص بفحص معين ممكن يجيني powder
+
+177
+00:13:33,670 --> 00:13:36,610
+عبارة عن solute و أنا بدي أضيفله solvent عشان
+
+178
+00:13:36,610 --> 00:13:42,080
+أحوله ل solutionممكن يكون عبارة عن محاليل مركزة و
+
+179
+00:13:42,080 --> 00:13:46,060
+أنا لازم أخففها محلول مركز و أخففه لحين أصل
+
+180
+00:13:46,060 --> 00:13:52,240
+التركيز الفعال للفحص طبيعي أو ممكن يكون عبارة عن
+
+181
+00:13:52,240 --> 00:13:56,580
+ready to use reagent، reagent محضر و جاهز و بس
+
+182
+00:13:56,580 --> 00:14:01,440
+عليا أنا أستخدمهبالنسبة للنوع الأخر من المحاليل هي
+
+183
+00:14:01,440 --> 00:14:07,640
+ال staining solution stain .. stain معناها يصبغ
+
+184
+00:14:07,640 --> 00:14:12,500
+تمام؟ staining solution محاليل الصباغة staining
+
+185
+00:14:12,500 --> 00:14:16,420
+solution هي عبارة عن solutions that contain
+
+186
+00:14:16,420 --> 00:14:22,230
+colored dyes فيهاصبغات ملونة تمام محاليل فيها
+
+187
+00:14:22,230 --> 00:14:27,070
+صبغات ملونة طبعا هذه المحاليل ممكن تكون مشحونة
+
+188
+00:14:27,070 --> 00:14:30,370
+وممكن تكون غير مشحونة ايش يعني مشحونة وغير مشحونة
+
+189
+00:14:30,370 --> 00:14:34,490
+يعني ممكن تكون عبارة عن basic يعني شحنتها موجبة
+
+190
+00:14:34,490 --> 00:14:38,910
+ممكن تكون عبارة عن تركيبتها acidic يعني هي حمض
+
+191
+00:14:38,910 --> 00:14:42,570
+يعني تركيبتها حمضية فبالتالي لها negative charge
+
+192
+00:14:42,570 --> 00:14:46,130
+او ممكن تكون neutral يعني لا basic ولا acidic
+
+193
+00:14:46,130 --> 00:14:50,820
+وبالتالي شحنتها صفرتمام؟ فحسب طب .. طب أنا إيش
+
+194
+00:14:50,820 --> 00:14:57,080
+بستفيد من هاي المعلومة؟ لإنه أنا هاي الصبغة بصبغ
+
+195
+00:14:57,080 --> 00:15:01,320
+فيها البكتيريا، خلايا البكتيريا و الفطريات و بصبغ
+
+196
+00:15:01,320 --> 00:15:06,580
+فيها التفايليات و خلايا الدم و خلايا جسم الإنسان
+
+197
+00:15:06,580 --> 00:15:10,960
+فبالتالي لازم إنه طبعا هذه الخلايا أنا مابقدرش
+
+198
+00:15:10,960 --> 00:15:14,140
+أشوفها إلا تحت ال microscope إلا أصبغها لازم
+
+199
+00:15:14,140 --> 00:15:19,580
+تمصبغتها علشان أقدر أشوفهاطيب هذه الجزيق .. طبعا
+
+200
+00:15:19,580 --> 00:15:23,440
+جزيقات الخلايا هذه مشحونة احنا عندنا في خلاياتنا
+
+201
+00:15:23,440 --> 00:15:27,260
+بروتينات موجبة الشحنة وبروتينات سالبة الشحنة ال
+
+202
+00:15:27,260 --> 00:15:32,700
+DNA اش اسمه ال DNA؟ ديوكسي رايبون نيوكلييك أسد
+
+203
+00:15:32,700 --> 00:15:37,100
+مثلا صح؟ ديوكسي رايبون نيوكلييك أسد من اسمه acid
+
+204
+00:15:37,100 --> 00:15:43,740
+تمام؟ يعني هو شحنته سالبةفأكيد بحتاج صبغة موجبة
+
+205
+00:15:43,740 --> 00:15:47,860
+عشان ترتبط معاه و هي تصبغه عشان هيك احنا لازم تكون
+
+206
+00:15:47,860 --> 00:15:54,100
+المحاليل اما مشحونة موجبة يعني تركبتها basic فبتها
+
+207
+00:15:54,100 --> 00:15:58,300
+تصبغلي الأشياء السالبة في الخلية تمام او تكون
+
+208
+00:15:58,300 --> 00:16:03,140
+acidic و تصبغلي الأشياء الموجبة في الخلية او تكون
+
+209
+00:16:03,140 --> 00:16:06,960
+neutral يعني غير مشحونة و شحنتها صفر و بتصبغلي
+
+210
+00:16:06,960 --> 00:16:12,140
+الأشياء الغير مشحونة داخل الخلية تمام؟طيب
+
+211
+00:16:12,140 --> 00:16:15,660
+different stain are used in medical laboratories
+
+212
+00:16:15,660 --> 00:16:19,760
+في عندي أنواع كتير من الصبغات و أنواع يعني متنوعة
+
+213
+00:16:19,760 --> 00:16:24,080
+من الصبغات اللي بتستخدم في المختبرات الطبية طبعا
+
+214
+00:16:24,080 --> 00:16:28,120
+من شأن هاي ال stain الصبغاتانها تعطيني artificial
+
+215
+00:16:28,120 --> 00:16:32,660
+color لون مصطنع يعني هو مش لون الخلية الأصلي لون
+
+216
+00:16:32,660 --> 00:16:36,940
+مصطنع لحتى تمكنني من ان انا اش اعمل اقدر اعمل
+
+217
+00:16:36,940 --> 00:16:42,360
+identification تمام بتخلي الخلايا او المواد المراد
+
+218
+00:16:42,360 --> 00:16:46,680
+رؤيتها مثلا تحت ال microscope او في العينة to be
+
+219
+00:16:46,680 --> 00:16:50,620
+identified علشان انا اقدر اميزها فبنكسبها لون
+
+220
+00:16:50,620 --> 00:16:54,580
+وطبعا يمكن صباغة العينات اللي هي عبارة عن ال blood
+
+221
+00:16:55,100 --> 00:16:59,100
+دم أو body fluid سواء الجسم ال urine عشان نشوف إيش
+
+222
+00:16:59,100 --> 00:17:02,840
+فيها مايكروبات أو بكتيريا أو تفايليات أو فطريات أو
+
+223
+00:17:02,840 --> 00:17:07,700
+خلايا حتى تمام؟ إذا النوع الآخر هو ال staining
+
+224
+00:17:07,700 --> 00:17:10,580
+solution تمام؟ إيش بدي أعرف عن ال staining
+
+225
+00:17:10,580 --> 00:17:14,100
+solution؟ أول حاجة بدي أعرف إن هو مكون من colored
+
+226
+00:17:14,100 --> 00:17:18,990
+dye من سبغات ملونة تمام؟ممكن يكون شحنته موجبة يعني
+
+227
+00:17:18,990 --> 00:17:23,470
+تركيبه basic او تكون شحنته لهذا المحلول يعني ال
+
+228
+00:17:23,470 --> 00:17:27,250
+standing solution تكون شحنته سالبة تمام يعني هو
+
+229
+00:17:27,250 --> 00:17:30,730
+تركيبه acidic او تكون شحنته صفر يعني هو neutral
+
+230
+00:17:30,730 --> 00:17:34,710
+متعادل لا هو basic ولا acidic طب انا ايش بستفيد من
+
+231
+00:17:34,710 --> 00:17:39,390
+ان انا اعرف شحنة ال solution بستخدمها الاستخدام
+
+232
+00:17:39,390 --> 00:17:43,230
+الصحيح يعني انا لو بدى اصبغ نوات الخلية نوات
+
+233
+00:17:43,230 --> 00:17:48,930
+الخلية مليانة أحمض نوويةما ليها نقاش أحماض نووية
+
+234
+00:17:48,930 --> 00:17:56,550
+DNA وRNA فطبعا بما أنها هي فيها أحماض نووية لازم
+
+235
+00:17:56,550 --> 00:18:00,430
+أن أنا أصبغها بال basic solution اللي هي المحليل
+
+236
+00:18:00,430 --> 00:18:05,110
+القاعديةطبعا هي بده تكون شحنتها سالبة لإنها أحمض
+
+237
+00:18:05,110 --> 00:18:11,570
+تمام و الصبغة هتكون شحنتها موجبة لإنها basic فموجب
+
+238
+00:18:11,570 --> 00:18:15,830
+مع سالب يتم التجاذب و الالتقاء و بالتالي الصبغة
+
+239
+00:18:15,830 --> 00:18:21,210
+راح ترتبط بجزء اللي مراد صباغته و بالتالي ممكن
+
+240
+00:18:21,210 --> 00:18:25,490
+بتمكنني من رؤيته تحت ال microscope فبالتالي هذا
+
+241
+00:18:25,490 --> 00:18:29,370
+اللي بستفيده من معرفة الشحنة و حكينا إنه أنا في
+
+242
+00:18:29,370 --> 00:18:32,990
+عندي أنواع كتيرمن الصبغات اللى تستخدم في ال
+
+243
+00:18:32,990 --> 00:18:36,430
+medical laboratories اللى بتعطى المواد المراد
+
+244
+00:18:36,430 --> 00:18:39,210
+رؤيتها او الأجزاء المراد رؤيتها تحت ال microscope
+
+245
+00:18:39,210 --> 00:18:43,370
+بتعطيها artificial color لون إصطناعى طبعا انا ممكن
+
+246
+00:18:43,370 --> 00:18:49,050
+أصبغ عينات الدم سواء الجسم ال urine الخلايا ال كل
+
+247
+00:18:49,050 --> 00:18:55,490
+إشي يعني مسحات البكتيريا أي نوع يعني عينة clinical
+
+248
+00:18:55,490 --> 00:18:59,310
+sample يعني عينة تم أخدها من مريض ممكن سبغتها
+
+249
+00:18:59,310 --> 00:19:03,930
+لرؤيتهاأو يعني اللي فحصها تحت ال microscope طيب
+
+250
+00:19:03,930 --> 00:19:08,810
+نوع التالت من المحاليل هو عبارة عن ال standard
+
+251
+00:19:08,810 --> 00:19:12,710
+solutions إيش يعني standard solution؟ طبعا
+
+252
+00:19:12,710 --> 00:19:17,130
+standard solution يعني محاليل معيارية إيش يعني
+
+253
+00:19:17,130 --> 00:19:21,210
+معيارية؟ يعني أنا بعاير ب .. ب .. ب .. يعني
+
+254
+00:19:21,210 --> 00:19:26,430
+باستخدام هذا المحلول كيف بعاير يعني؟يعني مثلا انا
+
+255
+00:19:26,430 --> 00:19:34,570
+العينة تبعتي هي فيها تركيز مجهولة تمام؟ و ال
+
+256
+00:19:34,570 --> 00:19:41,090
+standard solution بيكون تركيز معلوم تمام؟ طيب فال
+
+257
+00:19:41,090 --> 00:19:46,790
+.. التركيز هاي المعلومة انا بستخدمها يعني علشان
+
+258
+00:19:46,790 --> 00:19:52,260
+اعرف التركيز داخل العينةيعني انا بستخدم محاليل
+
+259
+00:19:52,260 --> 00:19:58,140
+معلومة التركيز علشان احصل علاقة التركيز المجهولة
+
+260
+00:19:58,140 --> 00:20:02,260
+داخل العينة اذا انا بجيب التركيز داخل العينة
+
+261
+00:20:02,260 --> 00:20:07,420
+بدلالة معلوم اللى وهو ال standard solution طيب كيف
+
+262
+00:20:07,420 --> 00:20:11,600
+بيصير هذا ال issue لنفرض مثلا خليني .. لنفرض ان
+
+263
+00:20:11,600 --> 00:20:18,660
+عندي محلول standard solution تمام وعينة مريضلو انا
+
+264
+00:20:18,660 --> 00:20:22,880
+اضفت عليهم ال reagent المادة المتفاعلة مثلا عشان
+
+265
+00:20:22,880 --> 00:20:29,120
+تتفاعل معله glucose وتعطيني لون، تمام؟ طيب فعليا
+
+266
+00:20:29,120 --> 00:20:33,780
+.. فعلياال standard solution أنا عارفة تركيزه هو
+
+267
+00:20:33,780 --> 00:20:36,980
+دايما بيكون معلوم التركيز لو نفرض انه تركيزه ..
+
+268
+00:20:36,980 --> 00:20:43,140
+تركيز السكر داخل المحلول هذا مية تمام و أنا ضفت
+
+269
+00:20:43,140 --> 00:20:48,100
+لهم reagent و أعطاني لون pink نفرض فلو أعطى في
+
+270
+00:20:48,100 --> 00:20:54,220
+العينة نص شدة اللون تمام نص شدة اللون اللي موجودة
+
+271
+00:20:54,220 --> 00:20:58,460
+في ال standard راح أعرف انه تركيز العينة تبعتي
+
+272
+00:20:58,460 --> 00:21:05,030
+خمسينطبعا لإن المعلوم مية لو أعطف العينة ضعف شدة
+
+273
+00:21:05,030 --> 00:21:11,970
+اللون عن ال standard رح أعرف إنه في العينة تبعتي
+
+274
+00:21:11,970 --> 00:21:18,570
+يعني تركيز متين ضعف طبعا إذا أنا في عندي جهاز
+
+275
+00:21:18,570 --> 00:21:22,350
+بيقيص الشدة اللون اللي بيظهر من التفاعل و بيقارن
+
+276
+00:21:22,350 --> 00:21:28,320
+ليها باستخدام ال standard solution و بالتاليبيقارن
+
+277
+00:21:28,320 --> 00:21:31,720
+ليها باستخدام standard solution وبالتالي بيحسبلي
+
+278
+00:21:31,720 --> 00:21:35,960
+التركيز المجهولة داخل العينة بدلالة معلوم تمام؟
+
+279
+00:21:35,960 --> 00:21:39,880
+طيب they are used to determine the value of an
+
+280
+00:21:39,880 --> 00:21:42,760
+identical chemical with unknown concentration of a
+
+281
+00:21:42,760 --> 00:21:46,200
+given solutionيعني احنا حكينا ان هاي المحاليل
+
+282
+00:21:46,200 --> 00:21:51,180
+تستخدم على حتى نعرف ايش to determine the value of
+
+283
+00:21:51,180 --> 00:21:54,060
+an identical chemical with a non-concentration
+
+284
+00:21:54,060 --> 00:21:59,420
+يعني انا بدي اعرف كمية تركيز مادة معينة مجهولة في
+
+285
+00:21:59,420 --> 00:22:03,500
+المحلول او في العينة تبعتيطيب ال .. ال .. ال
+
+286
+00:22:03,500 --> 00:22:06,580
+chemicals that are used to prepare these solutions
+
+287
+00:22:06,580 --> 00:22:10,280
+should be of analytical grade لازم المحاليل
+
+288
+00:22:10,280 --> 00:22:13,660
+المستخدمة في تحضير ال standard solution أو المواد
+
+289
+00:22:13,660 --> 00:22:17,660
+الكيميائية المستخدمة في تحضير ال standard solution
+
+290
+00:22:17,660 --> 00:22:20,780
+تكون of analytical grade إيش يعني analytical
+
+291
+00:22:20,780 --> 00:22:26,020
+grade؟ يعني على درجة عالية من الثباتوالدقة و
+
+292
+00:22:26,020 --> 00:22:31,800
+النقاء لحتى تستخدم اتمكنني ان انا استخدمها في فحص
+
+293
+00:22:31,800 --> 00:22:36,960
+طب و اجيب تركيز العينة المجهول بدلالة هذا المعلوم
+
+294
+00:22:36,960 --> 00:22:43,660
+تمام؟ طيبSince poor standard solution cause error
+
+295
+00:22:43,660 --> 00:22:46,960
+on the estimation of the intended substance, their
+
+296
+00:22:46,960 --> 00:22:52,640
+accuracy preparation is of poor amount تمام؟ إيش
+
+297
+00:22:52,640 --> 00:22:56,000
+يعني الكلام هذا؟ يعني بيحكيلك إنه إذا كان ال
+
+298
+00:22:56,000 --> 00:23:00,160
+standard solution تم تحضيره أو تخزينه أو التعامل
+
+299
+00:23:00,160 --> 00:23:05,400
+معاه بطريقة سيئة بحيث صار تالف تمام؟هذا راح يسببلي
+
+300
+00:23:05,400 --> 00:23:11,340
+errors أخطاء وخلل في عملية تقدير أو إيجاد تركيز
+
+301
+00:23:11,340 --> 00:23:15,920
+المواد المجهولة داخل العينة تمام importance in
+
+302
+00:23:15,920 --> 00:23:18,760
+order to obtain accurate and precise laboratory
+
+303
+00:23:18,760 --> 00:23:22,740
+finding in the medical laboratory إذن أهمية
+
+304
+00:23:22,740 --> 00:23:28,020
+standard solution تنبع من إنها هي بتمكننيمن الحصول
+
+305
+00:23:28,020 --> 00:23:33,220
+على accurate and precise laboratory findings يعني
+
+306
+00:23:33,220 --> 00:23:39,540
+نتائج تمام laboratory result نتائج دقيقة وصحيحة
+
+307
+00:23:39,540 --> 00:23:42,100
+accurate and precise laboratory finding أو
+
+308
+00:23:42,100 --> 00:23:46,160
+laboratory result يعني من خلال استخدام ال standard
+
+309
+00:23:46,160 --> 00:23:50,220
+solutionممكن أنا أصنف هذا ال standard solution إلى
+
+310
+00:23:50,220 --> 00:23:54,160
+نوعين أول نوع هو ال primary standard solution
+
+311
+00:23:54,160 --> 00:23:58,460
+والنوع التاني هو ال secondary standard solution
+
+312
+00:23:58,460 --> 00:24:01,820
+طيب خلينا نشوف إيش يعني primary standard solution
+
+313
+00:24:02,120 --> 00:24:05,680
+ال primary standard solution هو عبارة عن chemical
+
+314
+00:24:05,680 --> 00:24:10,140
+solution that has the highest purity and can be
+
+315
+00:24:10,140 --> 00:24:12,820
+used directly for the exact measurement of
+
+316
+00:24:12,820 --> 00:24:15,840
+substance of unknown concentration in a given
+
+317
+00:24:15,840 --> 00:24:20,000
+solution بحكيلك أنه ال primary standard solution
+
+318
+00:24:20,000 --> 00:24:24,080
+هو عبارة عن محلول كيميائي with the highest purity
+
+319
+00:24:24,080 --> 00:24:30,720
+يعني أعلى درجات النقاء والدقة يعني الفعلية تبعته
+
+320
+00:24:31,330 --> 00:24:37,850
+فبالتالي مباشرة بقدر انا استخدمه في إيجاد تركيز
+
+321
+00:24:37,850 --> 00:24:41,790
+مجهولة باستخدام المعلوم يعني بدلالة معلوم اللي هو
+
+322
+00:24:41,790 --> 00:24:45,990
+ال primary standard solution من أمثلة هذه المحاليل
+
+323
+00:24:45,990 --> 00:24:50,010
+ال sodium chloride ال sodium bicarbonate و ال
+
+324
+00:24:50,010 --> 00:24:55,710
+potassium iodide تمام؟ طيباش لازم يعني او كيف ال
+
+325
+00:24:55,710 --> 00:24:58,210
+.. اش الخصائص اللي لازم تتوفر في ال primary
+
+326
+00:24:58,210 --> 00:25:03,230
+standard solution بحكيلك لازم ان هو يكون مصنوع من
+
+327
+00:25:03,230 --> 00:25:07,370
+مواد اول حاجة free of impurities يعني خالية من
+
+328
+00:25:07,370 --> 00:25:13,210
+الشوائب لازم يكون highest pure يعني نقي جداهذا أول
+
+329
+00:25:13,210 --> 00:25:18,890
+خاصية أو أول صفة لازم يعني تتوفر في المواد المكونة
+
+330
+00:25:18,890 --> 00:25:21,650
+لل primary standard او المكونات لل primary
+
+331
+00:25:21,650 --> 00:25:26,050
+standard تاني شغلة stable on keeping in solid
+
+332
+00:25:26,050 --> 00:25:30,530
+state and in solution يعني لازم يكون هذا المحلول
+
+333
+00:25:30,530 --> 00:25:33,950
+أو المواد المكونة لل primary أو المصنعة أو اللي من
+
+334
+00:25:33,950 --> 00:25:38,730
+خلالها انا ب ..بكون او ب .. بصنع primary standard
+
+335
+00:25:38,730 --> 00:25:43,930
+solution لازم تكون هذه المواد ثابتة فيه سواء كانت
+
+336
+00:25:43,930 --> 00:25:47,450
+في الحالة الصلبة او السائلة كيف يعني؟ يعني لو كانت
+
+337
+00:25:47,450 --> 00:25:51,470
+لساتها بودر و انا لسه محضرتش منها primary standard
+
+338
+00:25:51,470 --> 00:25:55,990
+solution طبعا لازم تظل هذه المادة تكون ثابتة لو
+
+339
+00:25:55,990 --> 00:25:59,090
+انا بدأ أحطها في درجة الحرارة حرارة الغرفة او في
+
+340
+00:25:59,090 --> 00:26:04,300
+تلاجةأو في درجة حرارة التخزين و أيضا لو أنا حضرت
+
+341
+00:26:04,300 --> 00:26:09,380
+منها محلول لازم المحلول يظل ثابت التركيز طيب إيش
+
+342
+00:26:09,380 --> 00:26:13,640
+يعني مثلا لو أنا حطيت مادة اللي هى powder بتنتص
+
+343
+00:26:13,640 --> 00:26:19,430
+رطوبةفي درجة حرارة الغرفة فبالتالي هذه المادة اللي
+
+344
+00:26:19,430 --> 00:26:24,490
+بتنتص رطوبة رح يتغير مكوناتها فبالتالي هي لاتصلح
+
+345
+00:26:24,490 --> 00:26:27,830
+لإنها تكون مكون من مكونات ال primary standard
+
+346
+00:26:27,830 --> 00:26:32,370
+solution تمام هذا معناه انه يكون stable in keeping
+
+347
+00:26:32,370 --> 00:26:35,670
+in solid state او in solution لو انا حضرت محلول
+
+348
+00:26:35,670 --> 00:26:39,270
+على أساس ان هو primary standard solution واتفاجأت
+
+349
+00:26:39,270 --> 00:26:43,680
+انه بيصير له evaporation بيتبخرمعناها هذا اشماله
+
+350
+00:26:43,680 --> 00:26:47,960
+لا يصلح انه يكون primary standard solution هاي
+
+351
+00:26:47,960 --> 00:26:52,970
+معناه انه يكون stable ثابتطبعا .. طبعا لازم كمان
+
+352
+00:26:52,970 --> 00:26:56,330
+يكون able to be accurately weighed or measured
+
+353
+00:26:56,330 --> 00:27:01,330
+لازم طبعا انا اكون جادرة ان انا اقيس اوزنه او
+
+354
+00:27:01,330 --> 00:27:06,630
+اقيسه او اعرف تركيزه بشكل دقيق جدا لانه انا تركيزه
+
+355
+00:27:06,630 --> 00:27:12,610
+هذا المعلوم تمام بدي استخدمه عشان اجي .. اجد
+
+356
+00:27:12,610 --> 00:27:18,720
+بدلقته تركيز مجهول موجود داخل العينةطيب لازم ايضا
+
+357
+00:27:18,720 --> 00:27:22,880
+يكون not hygroscopic اش يعني hygroscopic يعني يمتس
+
+358
+00:27:22,880 --> 00:27:27,200
+رتوبة hygroscopic معناها يمتس رتوبة not
+
+359
+00:27:27,200 --> 00:27:32,340
+hygroscopic يعني لا يمتس رتوبة هذا شرط من شروط ال
+
+360
+00:27:32,340 --> 00:27:35,760
+primary standard solution doesn't absorb moisture
+
+361
+00:27:35,760 --> 00:27:40,360
+and vaporize حتى كمان مابيتبخرش على درجة حرارة
+
+362
+00:27:40,360 --> 00:27:44,600
+الغرفة او حتى درجة حرارة عشرينهذا يعني الصبات
+
+363
+00:27:44,600 --> 00:27:49,880
+الصبات وإنه يكون pure هذا الخصائص اللي لازم تتوفر
+
+364
+00:27:49,880 --> 00:27:53,420
+في ال primary standard solution هو عبارة عن ال
+
+365
+00:27:53,420 --> 00:27:58,740
+highest pure solution اللي أنا من خلاله بجيب تركيز
+
+366
+00:27:58,740 --> 00:28:02,840
+مجهول داخل العينة بدلال التركيز معلوم على و هو ال
+
+367
+00:28:02,840 --> 00:28:07,240
+primary standard solution بمشي زي و زي العينة بضيف
+
+368
+00:28:07,240 --> 00:28:12,300
+عليهم الاتنين reagent و براقب التغيرلو صار تغير في
+
+369
+00:28:12,300 --> 00:28:15,160
+العينة ضعف التغير اللي في ال .. في ال standard
+
+370
+00:28:15,160 --> 00:28:18,040
+معناها التركيز في العينة ضعف التركيز اللي في ال
+
+371
+00:28:18,040 --> 00:28:24,740
+standard لو صار تغير في العينة تلت التركيز اللي ..
+
+372
+00:28:24,740 --> 00:28:27,880
+تلت التغير اللي صار في ال standard معناها التركيز
+
+373
+00:28:27,880 --> 00:28:33,820
+في العينة تلت التركيز في ال standard و هكذا تمام؟
+
+374
+00:28:34,900 --> 00:28:38,660
+طيب secondary standard solution ال secondary
+
+375
+00:28:38,660 --> 00:28:44,460
+standard solution هي عبارة عن محليل أقل نقاء lower
+
+376
+00:28:44,460 --> 00:28:50,400
+impurity تمام و إذا ان هي مابتستخدم directly علشان
+
+377
+00:28:50,400 --> 00:28:55,440
+انا اجيس فيها او استخدمها كمعلوم و اجيب يعني
+
+378
+00:28:55,440 --> 00:29:00,620
+بدلالتها تركيز مجهول لأ طبعا هي .. انا كمان
+
+379
+00:29:00,620 --> 00:29:04,460
+مابكونش عارفة تركيز تبعها بجيبهان انا بقارنها ب
+
+380
+00:29:04,460 --> 00:29:09,500
+primary standard تمام؟ اذا هو محلول مش كتير بيوت و
+
+381
+00:29:09,500 --> 00:29:14,920
+مش معلوم تركيزه بقارنه ب .. بمحالي ال primary
+
+382
+00:29:14,920 --> 00:29:19,000
+standard بمحالي ال .. ال .. ال هي يعني ال number
+
+383
+00:29:19,000 --> 00:29:23,240
+one معلومة التركيز و بعد هيك ممكن انا استخدمها بال
+
+384
+00:29:23,240 --> 00:29:26,500
+analytical procedures زي في التجارب التحليلية و
+
+385
+00:29:26,500 --> 00:29:31,970
+المعايرة و الأشياء هايطيب من الأمثلة على هذه
+
+386
+00:29:31,970 --> 00:29:35,230
+المحاليين ال nitric acid ال hydrochloric acid و ال
+
+387
+00:29:35,230 --> 00:29:40,890
+sulphuric acid طبعا إيش .. و أنا بحضر secondary
+
+388
+00:29:40,890 --> 00:29:45,980
+standard solution يعني في اعتبارات يجب أنتراهاأول
+
+389
+00:29:45,980 --> 00:29:49,400
+حاجة بدى انا و انا بحضره لازم استخدم analytical
+
+390
+00:29:49,400 --> 00:29:54,320
+balance ل .. ل .. لأخذ الأوزان يعني لو قاللي هذه
+
+391
+00:29:54,320 --> 00:29:58,720
+المادة ال powder خدي منها خمسة جرام و داوبيهم مثلا
+
+392
+00:29:58,720 --> 00:30:02,520
+بميت أمقل distilled water عشان تحصلي على secondary
+
+393
+00:30:02,520 --> 00:30:06,420
+standard solution لازم انا عشان اوزن الخمسة جرام
+
+394
+00:30:06,420 --> 00:30:11,650
+هدول استخدم analytical balanceالميزان اللي من نوع
+
+395
+00:30:11,650 --> 00:30:16,970
+analytical اش يعني يعني ميزان حساس و دقيق جدا تمام
+
+396
+00:30:16,970 --> 00:30:20,810
+طيب dissolving the weighted substance in the
+
+397
+00:30:20,810 --> 00:30:24,610
+prescribed volume الخمسة جرام هدول حكولي او
+
+398
+00:30:24,610 --> 00:30:29,150
+التعليمات اللي تحكيلي ان انا ادوبهم ب 100 ml من ال
+
+399
+00:30:29,150 --> 00:30:33,110
+solvent خلاص مية يعني مية مابنفعش 110 ولا 120
+
+400
+00:30:33,110 --> 00:30:38,160
+نهائيا انا مابنفع ألعبي الحجومdetermining the
+
+401
+00:30:38,160 --> 00:30:41,420
+exact concentration by converging against a
+
+402
+00:30:41,420 --> 00:30:45,980
+primary solution بعد ما أكون حضرت وزنتي الوزنة
+
+403
+00:30:45,980 --> 00:30:50,480
+وضفت ال exact volume of solvent من ال secondary
+
+404
+00:30:50,480 --> 00:30:56,580
+standard solution لازم أروح أنا أجيب تركيزه يعني
+
+405
+00:30:56,580 --> 00:31:01,440
+أعمله زي كأنه مجهول التركيز و أجيب تركيزه بدلالة
+
+406
+00:31:01,440 --> 00:31:04,560
+المعلوم مين هو المعلوم عندي ال primary standard
+
+407
+00:31:04,560 --> 00:31:08,310
+اللي هو رقم one في ال solutionال highest purity و
+
+408
+00:31:08,310 --> 00:31:14,890
+ال .. ال accurate concentration له طبعا و بعد هيك
+
+409
+00:31:14,890 --> 00:31:20,510
+ممكن أنا أستخدمه إما ك .. يعني ك stock عندي يعني
+
+410
+00:31:20,510 --> 00:31:25,970
+محلول مركز أصير أعمل منه dilution يعني حين
+
+411
+00:31:25,970 --> 00:31:31,130
+الاستخدام أو إن أنا أحضره جاهز ready to use طبعا
+
+412
+00:31:31,750 --> 00:31:37,190
+فإذا إيش يعني stock يعني محلولي مركز تمام كمخزون
+
+413
+00:31:37,190 --> 00:31:42,490
+عندى مركز تمام و لحين الاستخدام أنا ب .. ب .. بحضر
+
+414
+00:31:42,490 --> 00:31:45,970
+التخفيف اللى بديه او التركيز اللى بلزمني فى الشغل
+
+415
+00:31:45,970 --> 00:31:52,460
+هى معناهاطيب النوع ال .. ال .. الرابع و الأخير من
+
+416
+00:31:52,460 --> 00:31:57,620
+المحاليل اللي تستخدم في المختبرات الطبية هي ال
+
+417
+00:31:57,620 --> 00:32:01,260
+buffer solution إيش يعني buffer؟ ال buffer هو
+
+418
+00:32:01,260 --> 00:32:07,240
+المحلول الذي يقاوم التغير في ال pHتمام ال buffer
+
+419
+00:32:07,240 --> 00:32:13,020
+is a solution that resist change in pH وغالبا مكون
+
+420
+00:32:13,020 --> 00:32:18,380
+مكون من weak acid مثلا و strong base او strong
+
+421
+00:32:18,380 --> 00:32:23,420
+acid و weak base تمام اذا اما weak acid و strong
+
+422
+00:32:23,420 --> 00:32:28,370
+base لازم يكون واحد فيهم قوي و واحد ضعيف تمامweak
+
+423
+00:32:28,370 --> 00:32:32,790
+acid with strong base او strong acid with weak
+
+424
+00:32:32,790 --> 00:32:37,730
+base وفي يعني .. يعني كتب بتعبر عن هالكلام هذا
+
+425
+00:32:37,730 --> 00:32:44,210
+باستخدام مصطلح salt مصطلح salt اش يعني؟ يعني مثلا
+
+426
+00:32:44,210 --> 00:32:50,950
+ال weak acid زي ال .. ال ..ال acidic acid طبعا ال
+
+427
+00:32:50,950 --> 00:32:57,410
+acidic acid weak acid هذا طيب ايش ال .. ال .. ال
+
+428
+00:32:57,410 --> 00:33:01,750
+base طبعه اللي هو عبارة عن ال acetate طبعا و هذه
+
+429
+00:33:01,750 --> 00:33:06,790
+strong base تعتبر فاما بقول عنها ال conjugate base
+
+430
+00:33:06,790 --> 00:33:10,690
+طبعا يعني ال acid و ال base طبعته او ال acid و ال
+
+431
+00:33:10,690 --> 00:33:14,670
+salt طبعته بس المهم اللي يبديكم تعرفوه ان ال
+
+432
+00:33:14,670 --> 00:33:18,780
+buffer يستخدم من weak acid او strongbase أو strong
+
+433
+00:33:18,780 --> 00:33:24,420
+acid weaknessتمام؟ و هو من خصائصه انه يقاوم التغير
+
+434
+00:33:24,420 --> 00:33:28,020
+بالبيئة resisted change in the pH طبعا ال buffer
+
+435
+00:33:28,020 --> 00:33:35,220
+يستخدم لما انا بدي .. لما بيكون ال pH يعني مهم جدا
+
+436
+00:33:35,220 --> 00:33:40,640
+انه يكون مضبوط في التفاؤلات تبعتي او في الفحوصات
+
+437
+00:33:40,640 --> 00:33:44,960
+تبعتي يعني مثلا لو انا بستخدم او بدي اقيس تركيز
+
+438
+00:33:44,960 --> 00:33:51,950
+انزيمات او نشاط انزيمات يعني في عينة معينةيعني
+
+439
+00:33:51,950 --> 00:33:55,570
+مثلا لو أنا بدي أجيس وظائف الكبد وظائف الكبد إيش
+
+440
+00:33:55,570 --> 00:33:58,850
+يعني وظائف الكبد؟ كتير بتسمع وظائف كبد ووظائف كلة
+
+441
+00:33:58,850 --> 00:34:02,230
+طب ما هي دي أصلا وظائف كبد ووظائف كلة هي عبارة عن
+
+442
+00:34:02,230 --> 00:34:05,930
+بحوصات أنا بجيس فيها نشاط الإنزيمات اللي موجودة
+
+443
+00:34:05,930 --> 00:34:10,050
+داخل الكبد أو موجودة داخل الكلة وبتمكن الكلة من
+
+444
+00:34:10,050 --> 00:34:13,770
+إنها تشتغل شغلها أو الكبد إنه يشتغل شغله عشان هيك
+
+445
+00:34:13,770 --> 00:34:17,910
+لو كانت الإنزيمات هذه نشاط طبعها أو ال .. ال .. ال
+
+446
+00:34:17,910 --> 00:34:22,820
+activity طبعتهافعال تمام بحكي انه وظائف الكبد
+
+447
+00:34:22,820 --> 00:34:27,320
+ممتازة و وظائف الكلا ممتازة تمام؟ طيب هذه
+
+448
+00:34:27,320 --> 00:34:30,320
+الانزيمات طبعا احنا بنعرف انه اي انزيم بال ..
+
+449
+00:34:30,320 --> 00:34:34,760
+بالدنيا لازم انه يشتغل عن درجة حرارة مناسبة و pH
+
+450
+00:34:34,760 --> 00:34:40,300
+مناسب و مايكونش فيه الو inhibitor ولا يعني معيق
+
+451
+00:34:40,300 --> 00:34:46,700
+لشغله تمام؟طيب فإذا لازم انا عشان أجيس نشاط
+
+452
+00:34:46,700 --> 00:34:52,240
+الانزيمات هذه أوفر ال pH المناسب ودرجة الحرارة
+
+453
+00:34:52,240 --> 00:34:56,060
+المناسبة لإنه الانزيم يكون فعال عشان أجيس نشاطه
+
+454
+00:34:56,060 --> 00:35:00,540
+فبالتالي بستخدم buffers علشان أوفر ال pH المناسب
+
+455
+00:35:00,540 --> 00:35:04,960
+لنشاط هذا ال enzymeفبالتالي يكون الفحص طبيعي شغال
+
+456
+00:35:04,960 --> 00:35:09,800
+أو الفحص طبيعي دقيق أو معبر عن نشاط الانزيم، بنفعش
+
+457
+00:35:09,800 --> 00:35:13,440
+.. يعني أنا مثلا في عندى في جسمى انزيمات الماعدة
+
+458
+00:35:13,440 --> 00:35:18,740
+بتشتغل عند ال .. ال .. ال .. البيئات ال acidic،
+
+459
+00:35:18,740 --> 00:35:23,510
+تمام؟و عندي انزيمات ال intestine اللي هي الأمعاء
+
+460
+00:35:23,510 --> 00:35:28,670
+بتشتغل عند ال pH ال alkaline ما بنفع انا اروح اخد
+
+461
+00:35:28,670 --> 00:35:35,990
+اقيس تركيز انزيمات المعدة و انا حاطة ال pH للتفاعل
+
+462
+00:35:35,990 --> 00:35:39,850
+9 او 10 طب ما المعدة اصلا ال pH تبعها 3 و
+
+463
+00:35:39,850 --> 00:35:44,610
+الانزيمات مصممة انها تشتغل على pH 3 ليش انا اقيسها
+
+464
+00:35:44,610 --> 00:35:49,730
+في تفاعل ال pH تبعه 8 او 9؟ غلطساعتها الانزيمات مش
+
+465
+00:35:49,730 --> 00:35:53,090
+هتشتغل و انا .. و انا افكر ان الانزيمات مضروبة
+
+466
+00:35:53,090 --> 00:35:56,930
+يعني في مشكلة في المعي ده فبالتالي ابدا اعطي نتائج
+
+467
+00:35:56,930 --> 00:36:02,250
+خاطئة و تفسير خاطئ و diagnosis خاطئ فمن .. يفضل ان
+
+468
+00:36:02,250 --> 00:36:08,230
+انا اوفر ال pH المناسب لنشاط ال enzyme علشان انا
+
+469
+00:36:08,230 --> 00:36:14,010
+لما اعبر عن ال activity تبعته اعبر بشكل دقيقتمام و
+
+470
+00:36:14,010 --> 00:36:18,270
+كيف انا بوفر ال pH المناسب لهذا ال enzyme باستخدام
+
+471
+00:36:18,270 --> 00:36:24,510
+ال buffer تمام باستخدام ال bufferطيب المحاليل هذه
+
+472
+00:36:24,510 --> 00:36:29,950
+طبعا ال .. ال .. احنا حكينا يعني انه قصة التركيز
+
+473
+00:36:29,950 --> 00:36:34,650
+او نقطة التركيز لهذه المحاليل مهمة جدا، طبعا فإذا
+
+474
+00:36:34,650 --> 00:36:38,970
+لازم انا يكون طبعا انه انا اعبر عن محاليل او تركيز
+
+475
+00:36:38,970 --> 00:36:44,130
+المحاليل بشكل accurate، طبعا و باستخدام ال .. ال
+
+476
+00:36:44,130 --> 00:36:48,180
+.. ال unit المناسبةيعني لما انا بدي احكي تركيز
+
+477
+00:36:48,180 --> 00:36:54,680
+المحلول هذا خمسة طب خمسة إيش؟ ما بعرف خمسة وخلاص
+
+478
+00:36:54,680 --> 00:36:59,180
+أسكت ما .. هذا تركيز غير معبر لأ لما لكن أحكي أنا
+
+479
+00:36:59,180 --> 00:37:05,100
+خمسة milligram per deciliter أو خمسة في المية
+
+480
+00:37:05,100 --> 00:37:11,080
+weight volume أو خمسة molar إذا انا هيك افهمت إيش
+
+481
+00:37:11,080 --> 00:37:16,950
+يعني تركيزتمام؟ مجرد ما أنا ذكرت ال .. الوحدة،
+
+482
+00:37:16,950 --> 00:37:21,370
+وحدة القياس، إذا أنا عرفت أو عبرت عن التركيز بشكل
+
+483
+00:37:21,370 --> 00:37:26,520
+كتير أفضل من ما أني أعبر عن القيمة فقطو اذا لازم
+
+484
+00:37:26,520 --> 00:37:30,200
+انا اعبر او يعني يكون ال concentration دقيق و اعبر
+
+485
+00:37:30,200 --> 00:37:34,620
+عنه بالوحدة المناسبة لإله طبعا في عندي نوعين من
+
+486
+00:37:34,620 --> 00:37:38,620
+الوحدات اللي تستخدم في التعبير عن القياس في وحدات
+
+487
+00:37:38,620 --> 00:37:41,820
+بسميها physical unit ال physical unit ايش يعني
+
+488
+00:37:41,820 --> 00:37:46,060
+physical unit؟لمّا أحكي أنه وحدات فيزيائية، إيش
+
+489
+00:37:46,060 --> 00:37:49,960
+يعني؟ يعني مثلا باستخدام الوزن والطول وال ..
+
+490
+00:37:49,960 --> 00:37:55,060
+والحجم هك .. هيك يعني باعبر فيزيائيا، تمام؟
+
+491
+00:37:55,060 --> 00:37:58,820
+الفيزيائيا يعني أنه مثلا هي أخدته يمكن بال .. بال
+
+492
+00:37:58,820 --> 00:38:04,080
+.. بالفيزيا أنه يعني بأول محاضرة أن كانت اللي هي
+
+493
+00:38:04,080 --> 00:38:09,140
+عن ال .. ال .. الوحدات المعياريةال .. ال .. الوزن
+
+494
+00:38:09,140 --> 00:38:15,020
+و الطول و الحجم ال .. المسافة هي الطول اه يعني زي
+
+495
+00:38:15,020 --> 00:38:20,260
+هيك فإذا انا باستخدام هاي الوحدات بعبر عنها فإذا
+
+496
+00:38:20,260 --> 00:38:22,920
+انا باستخدم بكون physical unit للتعبير عن ال
+
+497
+00:38:22,920 --> 00:38:28,400
+concentrationأول وحدة أو اول مثال هنستخدمه اللي هو
+
+498
+00:38:28,400 --> 00:38:32,260
+ال weight to volume ال weight to volume هو عبارة
+
+499
+00:38:32,260 --> 00:38:36,920
+عن ايش يعني؟ وزن ال solute بالنسبة لحجم ال
+
+500
+00:38:36,920 --> 00:38:41,400
+solvent، تمام؟ اذا وزن ال solute بالنسبة لحجم ال
+
+501
+00:38:41,400 --> 00:38:45,300
+solvent هادي بسميها weight to volume unitفي عندي
+
+502
+00:38:45,300 --> 00:38:49,620
+محلول مثلا هيماعطينيا بيحكيلي أنه تركيز محلول ل
+
+503
+00:38:49,620 --> 00:38:54,400
+glucose solution هو عبارة عن أربعين جرام في المية
+
+504
+00:38:54,400 --> 00:38:58,240
+weight to volume أربعين جرام في المية weight to
+
+505
+00:38:58,240 --> 00:39:02,300
+volume هذي لو أنا بدي أفسرها إيش يعني؟ يعني مثلا
+
+506
+00:39:02,300 --> 00:39:08,580
+أربعين جرام من ال weight تمام؟ أربعين جرام من ال
+
+507
+00:39:08,580 --> 00:39:15,040
+weight لمين؟ لل glucoseفي ايش؟ في ميت أمقل المية
+
+508
+00:39:15,040 --> 00:39:18,680
+بالمية هذه معناها مية في ميت أمقل من ال volume
+
+509
+00:39:18,680 --> 00:39:23,600
+لمين؟ لال solvent طبعا؟ اذا كانت ال .. ال .. لو
+
+510
+00:39:23,600 --> 00:39:26,800
+انا بدي احكي اربعين جرام في المية weight و volume
+
+511
+00:39:26,800 --> 00:39:30,720
+glucose solution ايش معناه الكلام هذا؟ معناه انه
+
+512
+00:39:30,720 --> 00:39:35,980
+في محلول glucose طبعا؟ محلول سكر تم تحضيره بذابة
+
+513
+00:39:35,980 --> 00:39:39,080
+اربعين جرام ال weight هذا اللي هي وزن ال .. ال
+
+514
+00:39:39,080 --> 00:39:43,580
+solute اربعين جراممن ال glucose ال powder طبعا
+
+515
+00:39:43,580 --> 00:39:49,900
+اضافتها او اذابتها في ايش؟ في مية طبعا volume يعني
+
+516
+00:39:49,900 --> 00:39:57,120
+في ميت ml منين؟ من ال ال solvent طبعا و هنا بستخدم
+
+517
+00:39:57,120 --> 00:40:00,700
+ايش؟ ال physical unit ال physical unit ل ال weight
+
+518
+00:40:00,700 --> 00:40:06,180
+ل ال weight جرام و ال physical unit ل ال volume ml
+
+519
+00:40:06,180 --> 00:40:07,300
+طبعا
+
+520
+00:40:11,300 --> 00:40:15,020
+طيب النوع الأخر لما أحكي weight to weight طبعا
+
+521
+00:40:15,020 --> 00:40:17,960
+weight .. weight to weight أو weight per weight
+
+522
+00:40:17,960 --> 00:40:23,220
+طيب إيش يعني .. يعني وزن السوليوت بالنسبة لوزن ال
+
+523
+00:40:23,220 --> 00:40:27,440
+solvent يعني مثلا هي هنا مثال معطيني تلاتين جرام
+
+524
+00:40:27,440 --> 00:40:30,560
+في المية weight to weight hydrochloric acid إيش
+
+525
+00:40:30,560 --> 00:40:34,680
+معناها؟ معناها إنه تم تحضير هذا المحلول باستخدام
+
+526
+00:40:34,680 --> 00:40:40,140
+تلاتين جرام من ال hydrochloric acid طبعا هذا ال ..
+
+527
+00:40:40,140 --> 00:40:50,370
+السوليوتباش كملنا للمية هنا كملنا للمية يعني حطيت
+
+528
+00:40:50,370 --> 00:40:58,120
+سبعين gram من ال solventطبعا فها ده بيفرج عن ال
+
+529
+00:40:58,120 --> 00:41:01,560
+weight to volume ال weight to volume ال 40 في 100
+
+530
+00:41:01,560 --> 00:41:06,140
+طبعا أما اللي .. اللي لما .. اللي لما تكون weight
+
+531
+00:41:06,140 --> 00:41:10,320
+to weight بتكون يعني تلاتين في المية معناها تلاتين
+
+532
+00:41:10,320 --> 00:41:15,840
+في سبعين هذا الفرق ما بينهم و ال volume per volume
+
+533
+00:41:15,840 --> 00:41:21,610
+او ال volume to volume ratioمعناها هنا حجم السوليط
+
+534
+00:41:21,610 --> 00:41:26,670
+بالنسبة لحجم ال solvent تسعين في المية volume to
+
+535
+00:41:26,670 --> 00:41:30,490
+volume إيثانول إيش معناها؟ معناها أنا حضرت إيثانول
+
+536
+00:41:30,490 --> 00:41:37,280
+solution حطيت تسعين جرام من الإيثانول علىعشرة امال
+
+537
+00:41:37,280 --> 00:41:41,620
+من ال .. ال solvent أو ال distilled water تمام؟
+
+538
+00:41:41,620 --> 00:41:45,780
+اذا لما بتكون الوحدات ال ratio weight to weight
+
+539
+00:41:45,780 --> 00:41:49,780
+تمام؟ او volume to volume لما يكون هذا ال ratio
+
+540
+00:41:49,780 --> 00:41:54,580
+متجانس volume to volume هي متجانس حجم لحجم وزن
+
+541
+00:41:54,580 --> 00:42:00,280
+لوزن تمام؟ بيتم التحضير عن طريق التكميل يعني انا
+
+542
+00:42:00,280 --> 00:42:06,400
+بحط تلاتينو بكمل للمية، تمام؟ بكمل .. يعني سبعين،
+
+543
+00:42:06,400 --> 00:42:10,680
+مابحطش مش تلاتين و مية، لأ، تلاتين و سبعين، بكمل،
+
+544
+00:42:10,680 --> 00:42:14,960
+تكمل ال volume to volume نفس الاشي، متجانس هيه،
+
+545
+00:42:14,960 --> 00:42:19,420
+حطيت تسعين وكملت للمية، ضفت عشرة فقط لغير لكن لما
+
+546
+00:42:19,420 --> 00:42:25,270
+يكون weight to volume، لأما بكملش هنا وات اربعين
+
+547
+00:42:25,270 --> 00:42:29,330
+جرام و بحط المية .. المية في المية هذه يعني مية
+
+548
+00:42:29,330 --> 00:42:33,210
+أمقال distilled water ما بكملش طبعا انا .. طبعا
+
+549
+00:42:33,210 --> 00:42:36,730
+هنا الاربعين جرام انا مش عارفة ان هم .. هم ماجدش
+
+550
+00:42:36,730 --> 00:42:41,720
+حجمهم عشان اكمل تكميل مابنفعش اكملتمام فبالتالي
+
+551
+00:42:41,720 --> 00:42:45,300
+الاربعين جرام بحط عليهم ال volume زي ما هو مية في
+
+552
+00:42:45,300 --> 00:42:49,120
+المية لكن لما تكون الوحدة متجانسة بقى هستخدم اللي
+
+553
+00:42:49,120 --> 00:42:54,540
+هي التكميل يعني ان انا ب .. ال final volume الوزن
+
+554
+00:42:54,540 --> 00:42:58,480
+النهائي في النهائية بده يكون في عندي ميت جرام
+
+555
+00:42:58,480 --> 00:43:03,860
+تلاتين وسبعين الوزن النهائي مية هنا تسعين وعشرة ال
+
+556
+00:43:03,860 --> 00:43:08,790
+final volume بده يكون مية تمام؟هي معنى ال ..
+
+557
+00:43:08,790 --> 00:43:12,930
+الوحدات الفيزيائية طبعا في عندي وحدات كيميائية
+
+558
+00:43:12,930 --> 00:43:17,370
+اللي هي تستخدم في التركيز المحلي اللي .. اللي
+
+559
+00:43:17,370 --> 00:43:22,310
+تستخدم في ال .. المختبرات زي ال acid و ال bases و
+
+560
+00:43:22,310 --> 00:43:26,770
+ال reagents و أحيانا ال standards و الأشياء هذه
+
+561
+00:43:26,770 --> 00:43:32,710
+طبعاأحيانا بنعبر عنها بال specific gravity أو ال
+
+562
+00:43:32,710 --> 00:43:36,630
+percentage by weight طبعا أو أحيانا بنعبر بال ..
+
+563
+00:43:36,630 --> 00:43:41,630
+بال .. ال moral .. ال morality و ال normality طبعا
+
+564
+00:43:41,630 --> 00:43:45,970
+ال morality و ال normality طبعا طيب ال molar
+
+565
+00:43:45,970 --> 00:43:51,620
+solutionالـ molar solution أو المحلول اللي انا بده
+
+566
+00:43:51,620 --> 00:43:57,000
+اعبر عنه بالـ molarity تمام؟ اخدنا قانون مهم جدا
+
+567
+00:43:57,000 --> 00:44:00,340
+اللي هو عبارة عن او أخدتوه انتوا بالتحليلية مار
+
+568
+00:44:00,340 --> 00:44:05,160
+عليكم اللي هو عبارة عن ال molarity تساوي ال number
+
+569
+00:44:05,160 --> 00:44:09,940
+of moles على ال volume per literتمام ال number of
+
+570
+00:44:09,940 --> 00:44:14,320
+moles على ال volume per liter هذا بسميها ايش بجيس
+
+571
+00:44:14,320 --> 00:44:18,860
+انا ال molarity تبعت المحلول ال normality تمام
+
+572
+00:44:18,860 --> 00:44:24,780
+solution اللي هو عبارة عن ال ال ال molecular
+
+573
+00:44:24,780 --> 00:44:29,830
+weight على ال equivalent number على ال nتمام؟ و
+
+574
+00:44:29,830 --> 00:44:34,230
+هذه أيضا اللي هي .. اللي بسميها ال Valency تمام؟
+
+575
+00:44:34,230 --> 00:44:38,810
+بسميها ال Valency فهي الفرق ما بين ال molarity و
+
+576
+00:44:38,810 --> 00:44:48,090
+ال normality طبعا هرح أصورلكم محاضرة و أنا بكتب و
+
+577
+00:44:48,090 --> 00:44:52,550
+بتركلكم يعني و ب .. ب .. أضربلكم أمتلة على حساب ال
+
+578
+00:44:52,550 --> 00:44:58,610
+molarity و حساب ال normality تمام؟و راح توضح
+
+579
+00:44:58,610 --> 00:45:01,230
+الفكرة أكتر و راح أكتب معاها القوانين و تكون
+
+580
+00:45:01,230 --> 00:45:05,370
+المحاضرة مصورة عندكم و موجودة كمرجع فإن شاء الله
+
+581
+00:45:05,370 --> 00:45:11,070
+مش راح تتصعبوا منها مش زي ذكر المعلومة هيك و نمر
+
+582
+00:45:11,070 --> 00:45:13,730
+عنها مرور الكرام، لأ راح يكون إن شاء الله الأشي
+
+583
+00:45:13,970 --> 00:45:18,350
+ملموس عندكم من خلال ان انا راح أصور محاضرة تصوير و
+
+584
+00:45:18,350 --> 00:45:24,910
+انا بكتب و بعطيكم أمثلة و أشياء حسابية لحتى انعمق
+
+585
+00:45:24,910 --> 00:45:30,090
+فاهم إيش يعني molarity و إيش يعني normality، تمام؟
+
+586
+00:45:30,090 --> 00:45:34,330
+طيب ال .. ال dilution of solution تخفي في المحاليل
+
+587
+00:45:35,510 --> 00:45:41,790
+أيضا الجزئية هاي راح ناخد النظر منها و جزئية
+
+588
+00:45:41,790 --> 00:45:50,310
+التطبيق اللي هي الحسابات أيضا راح نتركها للمحاضرة
+
+589
+00:45:50,310 --> 00:45:54,370
+اللي .. اللي راح أصورها تصوير بحيث أنه يكون كل شيء
+
+590
+00:45:54,370 --> 00:45:58,700
+واضح ان شاء الله بالنسبة للكمخلينا بس بالأول نشوف
+
+591
+00:45:58,700 --> 00:46:02,980
+إيش يعني dilution of solution dilution يعني تخفيف
+
+592
+00:46:02,980 --> 00:46:07,620
+المحاليل تخفيف المحاليل تتذكروا أول المحاضرة إيش
+
+593
+00:46:07,620 --> 00:46:12,400
+أنا حكيتلكم معلومة حكيتلكم إن المحاليل ممكن تجيني
+
+594
+00:46:12,400 --> 00:46:16,480
+ready to use جاهزة للاستخدام ممكن تجيني powder و
+
+595
+00:46:16,480 --> 00:46:19,700
+أنا بحلها يعني بستخدم .. بتكون solute و بديف
+
+596
+00:46:19,700 --> 00:46:24,680
+solvent تمام و ممكن تجيني concentrated تمام و أنا
+
+597
+00:46:24,680 --> 00:46:29,510
+بدنا نعملي لها dilutionتكون مركزة و انا بحضر منها
+
+598
+00:46:29,510 --> 00:46:34,370
+تخفيف او تركيز معين بحيث انه يكون هو اللي يتم
+
+599
+00:46:34,370 --> 00:46:39,430
+استخدامه و اعتماده في التجربة فإذا ال dilution هو
+
+600
+00:46:39,430 --> 00:46:45,790
+عبارة عن تخفيف التركيز او تقليل التركيز لمحلول
+
+601
+00:46:45,790 --> 00:46:51,710
+معين تمام مثال تجربة عندك في البيتلو جبتي كاسة مي
+
+602
+00:46:51,710 --> 00:46:59,550
+و حطيتي فيها معلقة سكر معلقة صغيرة سكر و حطيتي نص
+
+603
+00:46:59,550 --> 00:47:05,630
+الكاسة عبيتيها ميه ضوبت السكر منيح و تذوقتي الحلقه
+
+604
+00:47:05,630 --> 00:47:11,570
+تبعته هيكون حلو مضاق حلو تمام؟طيب لنفرض أنه إنتي
+
+605
+00:47:11,570 --> 00:47:17,590
+كملتي الكاسة للآخر و دوبتي منيح عملتي إذابة منيح و
+
+606
+00:47:17,590 --> 00:47:23,210
+تذوقتي منه إيش راح نلاحظ؟ راح نلاحظ إنه مذاق الحلا
+
+607
+00:47:23,210 --> 00:47:30,010
+صار أقل أخف ليش؟ لإنه تركيز السكر صار أقل تمام؟ و
+
+608
+00:47:30,010 --> 00:47:34,410
+هذا اللي بيشرح تعريف ال dilution ال dilution هو
+
+609
+00:47:34,410 --> 00:47:40,630
+عملية تمام؟العملية اللى من خلالها ال concentration
+
+610
+00:47:40,630 --> 00:47:45,530
+تركيز او ال activity او النشاط لمحلول معين بيتم
+
+611
+00:47:45,530 --> 00:47:51,790
+ايش خفضه او تقليله تمام بيتم فيها خفض او تقليل
+
+612
+00:47:51,790 --> 00:47:56,350
+تمام ال concentration تركيز او نشاط محلول معين من
+
+613
+00:47:56,350 --> 00:48:02,230
+خلال ان انا ايش اعمل ات ضفة solventأنا قللت تركيز
+
+614
+00:48:02,230 --> 00:48:08,010
+السكر تمام قللت مستوى الحلاء تبع السكر من خلال أن
+
+615
+00:48:08,010 --> 00:48:11,410
+أنا ضفت إيش المزيد من الماء كملت الكاسة لأخرها
+
+616
+00:48:11,410 --> 00:48:17,790
+فإذا أنا ضفت المزيد من ال solvent تمامو بالتالي
+
+617
+00:48:17,790 --> 00:48:22,770
+أنا هيك قللت من تركيز السكر تمام؟ الكاسة اللي كانت
+
+618
+00:48:22,770 --> 00:48:26,950
+ممتلقة لحد نصها هي مركزة أكتر من الكاسة الممتلقة
+
+619
+00:48:26,950 --> 00:48:32,370
+كاملة فبالتالي ال dilution التخفيف يعني أن أنا
+
+620
+00:48:32,370 --> 00:48:38,190
+أضيف solvent أكتر فبالتالي أقل ال concentration ال
+
+621
+00:48:38,190 --> 00:48:46,150
+dilutionهو طبعا ممكن التعبير عنه بإيش ال ratio of
+
+622
+00:48:46,150 --> 00:48:49,750
+concentrated or stuck material to the total final
+
+623
+00:48:49,750 --> 00:48:55,330
+volume of solution هو قانون عبارة عن اللي هي الحجم
+
+624
+00:48:55,330 --> 00:48:59,910
+اللي أنا أخدته من المادة المركزة على الحجم الكائن
+
+625
+00:48:59,910 --> 00:49:04,270
+نهائي بعد مضيفة ال solventو أيضا هذا القانون راح
+
+626
+00:49:04,270 --> 00:49:09,630
+نعبر عنه راح نكتب .. نكتبه في المحاضرة المصورة و
+
+627
+00:49:09,630 --> 00:49:13,850
+راح نضرب عليه أمثلة و نشوف كيف احنا ممكن نعبر عن
+
+628
+00:49:13,850 --> 00:49:18,510
+هذا ال delusion و كيف انا ممكن اخد تطبيقات و أمثلة
+
+629
+00:49:18,510 --> 00:49:22,990
+حسابية على هذا القانون و كيف ممكن انا استخدمه في
+
+630
+00:49:22,990 --> 00:49:27,030
+حل المسائل الحسابية و لو مثلا عندي تركيز معين كيف
+
+631
+00:49:27,030 --> 00:49:30,850
+انا ممكن احضر منه تركيز آخرmany of the laboratory
+
+632
+00:49:30,850 --> 00:49:35,750
+procedures involve the use of dilution طبعا كتير
+
+633
+00:49:35,750 --> 00:49:40,350
+يعني المختبرات و تجارب المختبرات و أداء داخل
+
+634
+00:49:40,350 --> 00:49:45,150
+المختبرات بيحتاج التخفيف بيحتاج إجراء تخفيف لازم
+
+635
+00:49:45,150 --> 00:49:48,910
+أخفف العين أحيانا عشان يمكن قياسها لازم أخفف ال
+
+636
+00:49:48,910 --> 00:49:53,240
+reagent عشان يصير فعال و هكذافإذا أنا محتاجة
+
+637
+00:49:53,240 --> 00:49:58,260
+التخفيف بشكل كتير كبير و طبعا اللي بيفهم التخفيف
+
+638
+00:49:58,260 --> 00:50:03,700
+هنا في مادة المقدمة بيسهل عليه في المعامل الأخرى
+
+639
+00:50:03,700 --> 00:50:09,580
+في كل المواد بعدها انه لما يتطرقوا لجزئية ال
+
+640
+00:50:09,580 --> 00:50:13,080
+dilution يكون هو فاهمهاit's important to
+
+641
+00:50:13,080 --> 00:50:15,640
+understand the concept of dilution عشان هيك لازم
+
+642
+00:50:15,640 --> 00:50:20,460
+افهم انا ايش مبدأ ال dilution علشان بعد هيك في كل
+
+643
+00:50:20,460 --> 00:50:24,920
+المختبرات اكون فاهمة انا ايش بشتغل و كيف ممكن انا
+
+644
+00:50:24,920 --> 00:50:29,000
+احضر dilution من مادة مركزة و كيف انا استخدمها
+
+645
+00:50:29,000 --> 00:50:33,900
+طبعا these dilution have to be considered as they
+
+646
+00:50:33,900 --> 00:50:38,000
+make a quantitative difference what is going on
+
+647
+00:50:38,000 --> 00:50:44,830
+طبعا هي مما انه انا بلعب بالتركيزو بخفف تركيز عينة
+
+648
+00:50:44,830 --> 00:50:49,830
+معينة فبالتالي انا بعمل ايش؟ تغيير quantitative
+
+649
+00:50:49,830 --> 00:50:56,090
+تغيير كم على العينة طبعا ال dilution is made to
+
+650
+00:50:56,090 --> 00:51:00,470
+prepare بستخدم هذا ال dilution عشان لو كان في عندى
+
+651
+00:51:00,470 --> 00:51:05,030
+stockقبل شوية ذكرنا إيش يعني stock تمام لو كان
+
+652
+00:51:05,030 --> 00:51:08,770
+عندي stock solution مادة مركزة أحضر منها working
+
+653
+00:51:08,770 --> 00:51:14,810
+solution يعني أحضر منها تخفيف جاهز للاستخدام أو ل
+
+654
+00:51:14,810 --> 00:51:17,470
+measurable concentration of sample يعني مثلا
+
+655
+00:51:17,470 --> 00:51:22,270
+أحيانا بتكون العينة تركيز السكر فيها خمسمية يعني
+
+656
+00:51:22,270 --> 00:51:27,430
+كتير عالي و ال kit عندي أو المادة المتفاعلة بتجيس
+
+657
+00:51:27,430 --> 00:51:33,740
+لحد يعني أقصها لحد تلاتمية طب أنا إيش أعمل؟المادة
+
+658
+00:51:33,740 --> 00:51:36,840
+المتفاعلة مش هتجيس للنسبة للسكر لإن السكر في
+
+659
+00:51:36,840 --> 00:51:41,520
+العينة أكتر مش هتكون النتيجة معبرة عن العينة فانا
+
+660
+00:51:41,520 --> 00:51:46,340
+عشان أعمل أروح أخف في العينة تمام علشان أنزل السكر
+
+661
+00:51:46,340 --> 00:51:51,660
+اللي فيها وبالتالي أقدر أنا أجيسها باستخدام المادة
+
+662
+00:51:51,660 --> 00:51:54,950
+المتفاعلة اللي موجودة عندىلأن المادة المتفاعلة
+
+663
+00:51:54,950 --> 00:51:59,830
+السعة تبعتها او ال capacity تبعتها او قدرتها انها
+
+664
+00:51:59,830 --> 00:52:04,010
+تكشف ل 300 و العينة فيها 500 ايش الحل اخفف العينة
+
+665
+00:52:04,010 --> 00:52:08,990
+تمام؟ عشان تصير العينة اشمالها measurable يمكن
+
+666
+00:52:08,990 --> 00:52:14,530
+قياسهاطيب في عندي نوعين من ال .. من ال dilution
+
+667
+00:52:14,530 --> 00:52:20,510
+اللي هو واحد اسمه simple dilution تمام؟ و اللي هو
+
+668
+00:52:20,510 --> 00:52:24,790
+يعني مجرد مثلا انا اخد من مادة مركزة حجم معين و
+
+669
+00:52:24,790 --> 00:52:28,450
+اضيف عليه حجم اخر من ال solvent و هيك انا عملت
+
+670
+00:52:28,450 --> 00:52:32,580
+simple dilution تمام؟أما ال serial dilution هو
+
+671
+00:52:32,580 --> 00:52:36,840
+عبارة عن multiple و progressive dilution يعني مثلا
+
+672
+00:52:36,840 --> 00:52:40,220
+أنا كان عندي مادة مركزة أخدت منها حجم وضفت solvent
+
+673
+00:52:40,220 --> 00:52:44,500
+في أنبوبة رقم واحد بعدين أخدت من أنبوبة رقم واحد
+
+674
+00:52:44,500 --> 00:52:48,300
+حجم معين وحطته في تيوبة رقم اتنين وضفت عليها
+
+675
+00:52:48,300 --> 00:52:53,660
+solvent بعدين أخدت حجم من تيوبة رقم اتنين وضفته في
+
+676
+00:52:53,660 --> 00:52:56,880
+تيوبة رقم تلاتة وضفت عليها solvent فانا اش ما
+
+677
+00:52:56,880 --> 00:53:02,340
+عامالي بخفف أكتر و أكتر و أكترمن ال .. المادة
+
+678
+00:53:02,340 --> 00:53:07,160
+المراد عمل تخفيف لإلها و من استخدام ايش؟
+
+679
+00:53:07,160 --> 00:53:10,080
+بالاستخدام multiple dilutions انا استخدمت tu برقم
+
+680
+00:53:10,080 --> 00:53:13,400
+واحد بعد tu برقم اتنين بعد tu برقم تلاتة و هكذا
+
+681
+00:53:13,400 --> 00:53:17,080
+فبالتالي انا حصلت على multiple dilutions ففي
+
+682
+00:53:17,080 --> 00:53:21,500
+النهاية ال .. هذا بسميه serial dilution اللي هو
+
+683
+00:53:21,500 --> 00:53:25,880
+التخفيف المتسلسل من tu برقم واحد لاتنين لتلاتة و
+
+684
+00:53:25,880 --> 00:53:33,710
+هكذاطيب و أيضا ممكن انا استخدمه علشان انا لو كان ب
+
+685
+00:53:33,710 --> 00:53:38,190
+.. لو كان عندي .. بدي محاليل كتير تركيزها قليل او
+
+686
+00:53:38,190 --> 00:53:41,970
+علشان انا لو بدي احصل على محاليل ب .. في .. يعني
+
+687
+00:53:41,970 --> 00:53:47,870
+الفروق ما بينها ثابتة في ال .. التركيز تمام؟ هو
+
+688
+00:53:47,870 --> 00:53:51,910
+عبارة عن multiple حكينا و ال total dilution هو the
+
+689
+00:53:51,910 --> 00:53:55,490
+product of each dilution in the seriesهو ال total
+
+690
+00:53:55,490 --> 00:54:00,690
+dilution يعني التخفيف النهائي هو مجموع ضرب كل ال
+
+691
+00:54:00,690 --> 00:54:04,930
+dilution تبع الأنابيب كلها هنحكي إيش يعني بالظبط
+
+692
+00:54:04,930 --> 00:54:10,590
+تمام ال serial dilution بيبدأ في البداية بيكون
+
+693
+00:54:10,590 --> 00:54:14,210
+عبارة عن simple يعني أنا عندي stock تمام أخدت منه
+
+694
+00:54:14,210 --> 00:54:17,830
+حجم وحطت عليه solvent فتيوبة رقم واحد التيوبة رقم
+
+695
+00:54:17,830 --> 00:54:23,420
+واحد لحالة هاي بسميها simple dilution تمامو بعد
+
+696
+00:54:23,420 --> 00:54:27,200
+هيك التخفيفات التانية اللي في تيوب رقم اتنين و
+
+697
+00:54:27,200 --> 00:54:30,740
+تلاتة و هكذا هدول أشمع لهم بسميهم subsequent
+
+698
+00:54:30,740 --> 00:54:38,260
+dilutions ويعني تخفيفات متتابعة تيوب رقم واحد وما
+
+699
+00:54:38,260 --> 00:54:43,180
+يليها كلهم مع بعضهم بسميهم serial dilutionتمام
+
+700
+00:54:43,180 --> 00:54:48,120
+بسميهم serial dilution it has been known that if
+
+701
+00:54:48,120 --> 00:54:52,280
+we use larger volume we obtain a more accurate
+
+702
+00:54:52,280 --> 00:54:58,620
+dilution اذا كان .. اذا انا استخدمت حجوم اكتر يعني
+
+703
+00:54:58,620 --> 00:55:02,640
+حجوم اكبر بحصل على accurate dilution يعني مثلا لو
+
+704
+00:55:02,640 --> 00:55:09,960
+في النهاية لو انا بدي احصل على يعني بدي واحد لألف
+
+705
+00:55:09,960 --> 00:55:16,940
+واحد لألفdelution يعني أخد one microliter و أح ..
+
+706
+00:55:16,940 --> 00:55:22,100
+و أحطهم على تسعمية وتسعة وتسعين microliter مينفعش
+
+707
+00:55:22,100 --> 00:55:25,600
+واحد microliter يعني الحجم لا يكاد يورا بالعين
+
+708
+00:55:25,600 --> 00:55:30,930
+المجردة تمام؟فغلط طب لأ انا ليش ما اضاعف الكمية
+
+709
+00:55:30,930 --> 00:55:34,410
+اخد مثلا واحد امال بدل ال microliter و احطه في
+
+710
+00:55:34,410 --> 00:55:37,870
+تسعمية وتسعة وتسعين امال هيك بيكون احسن اذا انا
+
+711
+00:55:37,870 --> 00:55:42,410
+لازم لما بدي صح انا بكون بدي استخدم حجوم قليلة في
+
+712
+00:55:42,410 --> 00:55:46,370
+التفاعلات تبعتي لكن احضر ال dilution بكميات اكبر
+
+713
+00:55:46,370 --> 00:55:50,070
+بيكون التفاعل اضاق او يعني التركيز اللي انا بحصل
+
+714
+00:55:50,070 --> 00:55:54,090
+عليها او ال dilution اللي بحصل عليه اكثر دقةتمام
+
+715
+00:55:54,090 --> 00:55:57,190
+لما بدى اعمل performing serial dilution in the
+
+716
+00:55:57,190 --> 00:56:01,710
+clinical laboratory one must know the dilution of
+
+717
+00:56:01,710 --> 00:56:07,270
+each tube لازم اعرف التركيز لكل نبوبة داخل ال ..
+
+718
+00:56:07,270 --> 00:56:16,630
+ال ..العينة تبعتي طيب و بالتالي هذا طبعا هدول ال
+
+719
+00:56:16,630 --> 00:56:20,770
+.. هدول الأمثلة انا ان شاء الله راح أصور محاضرة و
+
+720
+00:56:20,770 --> 00:56:27,230
+أنا بشرحلكم كيف انا بحصل على التركيز ل .. ل ..
+
+721
+00:56:27,230 --> 00:56:33,760
+يعنيش اسمه simple dilution و التركيز كيف انا ايضا
+
+722
+00:56:33,760 --> 00:56:37,200
+برضه بحصل عليها ل serial dilution يعني كيف انا
+
+723
+00:56:37,200 --> 00:56:40,160
+بطبق لو أجاتكم مسائل على ال primary dilution او
+
+724
+00:56:40,160 --> 00:56:42,820
+اللي هو ال simple dilution او على ال serial
+
+725
+00:56:42,820 --> 00:56:46,500
+dilution كيف انتوا تشتغلوا و هنحكي عن الأمثلة هذه
+
+726
+00:56:46,500 --> 00:56:52,710
+و نتناولها بشكل دقيق يعني أفضللمّا أكون أنا بشرح و
+
+727
+00:56:52,710 --> 00:56:56,310
+بكتب بنفس الوقت أفضل من ما اني بس أشار عليها بال
+
+728
+00:56:56,310 --> 00:57:01,290
+mouse علشان يكون هيك عمقنا الفهم عندكم أكتر، لذا
+
+729
+00:57:01,290 --> 00:57:04,790
+هذه المحاضرة يتبعها محاضرة مصورة ان شاء الله ان
+
+730
+00:57:04,790 --> 00:57:10,190
+تدروها راح .. ان شاء الله راح تظهر لعندكم و
+
+731
+00:57:10,190 --> 00:57:14,330
+بالتالي يعني راح أحملكم إياها drive و بالتالي
+
+732
+00:57:14,330 --> 00:57:18,910
+تدرسوهم التنتين مع بعض علشان تتضح الصورة لديكم
+
+733
+00:57:19,510 --> 00:57:25,570
+يعطيكم العافية و ان شاء الله بنشوفكم يعني أو
+
+734
+00:57:25,570 --> 00:57:29,130
+بننزلكم المحاضرة المصورة قريبا
+
diff --git a/PL9fwy3NUQKwY9jl8xw-bWkVZkWcGGyspd/Bu-y4fC_shI.srt b/PL9fwy3NUQKwY9jl8xw-bWkVZkWcGGyspd/Bu-y4fC_shI.srt
new file mode 100644
index 0000000000000000000000000000000000000000..30c1ea9a71e4a99161e9c24718eede88db0b169e
--- /dev/null
+++ b/PL9fwy3NUQKwY9jl8xw-bWkVZkWcGGyspd/Bu-y4fC_shI.srt
@@ -0,0 +1,3422 @@
+1
+00:00:02,830 --> 00:00:06,730
+السلام عليكم ورحمة الله وبركاته، طالبات العزيزات
+
+2
+00:00:06,730 --> 00:00:09,970
+اليوم، إن شاء الله، راح نشرح محاضرة erythrocyte
+
+3
+00:00:09,970 --> 00:00:14,570
+sedimentation rate erythrocyte معناها red blood
+
+4
+00:00:14,570 --> 00:00:19,980
+cell، خلايا الدم الحمراء، sedimentation معناها ترسيب
+
+5
+00:00:19,980 --> 00:00:25,240
+rate معدل، إذاً ما عملنا اليوم بعنوان erythrocyte
+
+6
+00:00:25,240 --> 00:00:29,500
+sedimentation rate، أي معدل ترسيب خلايا الدم
+
+7
+00:00:29,500 --> 00:00:35,900
+الحمراء، فيما يعرف بـ ESR، تمام؟ اختصاراً لـ ESR قبل
+
+8
+00:00:35,900 --> 00:00:39,600
+ما نعرض الـ slides، خلونا بس مع بعض نشوف هاي الصورة
+
+9
+00:00:39,600 --> 00:00:43,340
+و هاي الصورة طبعاً مش غريبة علينا يا بنات، احنا
+
+10
+00:00:43,340 --> 00:00:47,560
+عرضناها في المعامل السابقة، و تحدثنا عن الـ blood
+
+11
+00:00:47,560 --> 00:00:51,140
+component، حكينا لو أنا عندي عينة blood في tube
+
+12
+00:00:51,140 --> 00:00:55,620
+وفصلتها، عملتلها separation on centrifuge باستخدام
+
+13
+00:00:55,620 --> 00:01:00,900
+جهاز الطرد المركزي، فرح تفصل العينة إلى 3 طبقات
+
+14
+00:01:01,150 --> 00:01:04,170
+الطبقة اللي بتكون موجودة في البطن هي طبقة الـ red
+
+15
+00:01:04,170 --> 00:01:08,790
+blood cell، على أساس إنها هي الـ red blood cell أو
+
+16
+00:01:08,790 --> 00:01:15,710
+الـ RBCs، على أساس إن هاي الطبقة بتكون الأسفل، تمام، و
+
+17
+00:01:15,710 --> 00:01:22,030
+تحتل ما بين 44 إلى 45% من الـ total volume of whole
+
+18
+00:01:22,030 --> 00:01:26,690
+blood، و يليها طبقة الـ buffy coat، اللي هي الطبقة
+
+19
+00:01:26,690 --> 00:01:31,570
+الكريمية اللي بتكون مكونة من، أو يعني تحتوي على الـ
+
+20
+00:01:31,570 --> 00:01:35,650
+white blood cell and the platelet، طبعاً خلايا الدم
+
+21
+00:01:35,650 --> 00:01:41,370
+البيضاء و الصفائح الدموية، هذه الطبقة لا تتجاوز الـ
+
+22
+00:01:41,370 --> 00:01:46,450
+1%، طبعاً، أو أحياناً نحكي مجازاً نحكي إنها 1% من الـ
+
+23
+00:01:46,450 --> 00:01:51,590
+total blood volume أو الـ whole blood volume، طيب و
+
+24
+00:01:51,590 --> 00:01:57,850
+يليها هاي طبقة طبقة الـ plasma، اللي بتحتل 55% من الـ
+
+25
+00:01:57,850 --> 00:02:01,730
+whole blood volume، و هي طبعاً بتكون موجودة at the
+
+26
+00:02:01,730 --> 00:02:06,600
+top، على أساس إنها هي أخف طبقة، طبعاً؟ طيب بس اليوم
+
+27
+00:02:06,600 --> 00:02:09,920
+أنا مش عم بذكر هاي الـ .. الـ .. أو مش عم بعرض هاي
+
+28
+00:02:09,920 --> 00:02:17,680
+الصورة لحتى فقط نذكر مكونات الـ .. الـ .. الدم لأَنَّ
+
+29
+00:02:17,680 --> 00:02:23,280
+أنا اليوم بدي .. بدي أحكي إنَّ عينة الدم طالما إنَّه
+
+30
+00:02:23,280 --> 00:02:27,960
+أنا سحبتها و عملتلها mixing، يعني وضعتها في tube و
+
+31
+00:02:27,960 --> 00:02:32,240
+عملتلها mixing مع anticoagulant، Anticoagulant يعني
+
+32
+00:02:32,240 --> 00:02:37,580
+مانع للتخثر، سواء كان هذا مانع التخثر EDTA أو
+
+33
+00:02:37,580 --> 00:02:42,420
+citrate أو heparin أو أي نوع من أنواع موانع التخثر
+
+34
+00:02:42,420 --> 00:02:46,840
+اللي تم ذكرها، أو يعني موجودة في محاضرة الـ blood
+
+35
+00:02:46,840 --> 00:02:53,290
+component، تمام؟ إذا تم سحب عينة و عملنا لها mixing
+
+36
+00:02:53,290 --> 00:02:57,690
+مع anticoagulant، يعني منعنا تخثر هذه العينة، منعنا
+
+37
+00:02:57,690 --> 00:03:02,130
+تجلطها، إذا بيبقى الدم في الصورة السائلة، وإذا
+
+38
+00:03:02,130 --> 00:03:06,530
+بقى الدم في الصورة السائلة، فهو يا بنات، يعني
+
+39
+00:03:06,530 --> 00:03:10,230
+tend to يميل إلى أنّه يفصل، لو أنا حطيته على الـ bench
+
+40
+00:03:10,230 --> 00:03:14,490
+لمدة يعني عشر دقائق، ربع ساعة، بيكون بلش يفصل، طبعاً
+
+41
+00:03:14,490 --> 00:03:20,530
+أنا بستخدم الـ centrifuge لحتى أسرّع العملية هذه
+
+42
+00:03:20,530 --> 00:03:24,810
+دقيقة، ثنتين، ثلاث، خمس دقائق، أنا بكون انتهيت من عملية
+
+43
+00:03:24,810 --> 00:03:30,930
+الفصل، طبعاً، لكن الـ blood في الصورة السائلة، هو
+
+44
+00:03:30,930 --> 00:03:35,230
+يميل إلى أنّه يفصل، بحيث أن الـ RBC بفعل الجاذبية
+
+45
+00:03:35,230 --> 00:03:39,770
+بتترسب لتحت، و بتفصل الـ plasma at the top، يعني بشكل
+
+46
+00:03:39,770 --> 00:03:44,060
+طبيعي، بس بدها تأخذ وقت هذه العملية، طبعاً، و هذه هي
+
+47
+00:03:44,060 --> 00:03:50,760
+فكرة فحصنا لليوم، يعني فكرة فحصنا لليوم بتحكي إنَّه
+
+48
+00:03:50,760 --> 00:03:56,500
+خلينا نرجع للصورة هاي، الـ ESR، الـ erythrocyte
+
+49
+00:03:56,500 --> 00:04:00,880
+sedimentation rate، فكرة الفحص طبعاً إنَّه أنا باستخدام
+
+50
+00:04:00,880 --> 00:04:05,420
+أنابيب خاصة يا بنات، و ركزولي على كلمة أنابيب خاصة
+
+51
+00:04:05,420 --> 00:04:08,700
+راح نذكر إيش أنواع الأنابيب اللي أنا بستخدمها
+
+52
+00:04:08,700 --> 00:04:14,800
+لاحقاً، ولكن باستخدام أنابيب خاصة، أنا بجمع عينة، عينة
+
+53
+00:04:14,800 --> 00:04:19,900
+whole blood، دم، و بعملها mixing مع anticoagulant،
+
+54
+00:04:19,900 --> 00:04:23,440
+يفضّل إنّي أستخدم الـ citrate، الـ sodium citrate
+
+55
+00:04:23,440 --> 00:04:28,700
+anticoagulant، و بعد هيك أنا بحطها على الـ bench
+
+56
+00:04:28,700 --> 00:04:33,400
+بشكل عمودي، تمام؟ بشكل عمودي، يعني إنها تصنع مع الـ
+
+57
+00:04:33,400 --> 00:04:38,240
+bench زاوية 90 درجة، تمام؟ لازم تكون vertical أو
+
+58
+00:04:38,240 --> 00:04:41,410
+perpendicular to the bench، تمام؟ بحطها في rack و
+
+59
+00:04:41,410 --> 00:04:45,590
+الـ rack بأضمن إنها هي .. هاي الـ .. الـ .. الأنبوبة بت
+
+60
+00:04:45,590 --> 00:04:51,390
+.. بشكل .. باتجاه عمودي، و تصنع زاوية 90 درجة مع
+
+61
+00:04:51,390 --> 00:04:56,270
+المستوى اللي .. اللي هي موجودة عليه، و بستنى عليها
+
+62
+00:04:56,270 --> 00:05:00,600
+ساعة، لأنّ حكينا اللي هو الـ blood في صورته السائلة
+
+63
+00:05:00,600 --> 00:05:05,440
+يميل إلى إنّه يفصل، فالـ RBCs راح تتجه إلى الـ bottom
+
+64
+00:05:05,440 --> 00:05:13,160
+و الـ plasma راح تفصل، بالنسبة للقراءة، أو النتيجة
+
+65
+00:05:13,160 --> 00:05:18,520
+اللي أنا بدي أقرأها، أنا طبعاً بقيس، أو يعني بدي قراءة
+
+66
+00:05:18,520 --> 00:05:23,340
+rate، معدل، يعني سرعة، تمام؟ و احنا يمكن أخذنا
+
+67
+00:05:23,340 --> 00:05:28,220
+بالفيزياء إنّ السرعة هي عبارة عن المسافة على الزمن
+
+68
+00:05:28,220 --> 00:05:34,500
+تمام؟ طيب، إذن طبعاً أنا بما إني بدي معدل ترسيب الـ
+
+69
+00:05:34,500 --> 00:05:38,820
+RBCs، إذا أنا بدي المسافة اللي تقطعها الـ RBCs لما
+
+70
+00:05:38,820 --> 00:05:44,180
+بتترسب على الزمن، تمام؟ إذا أنا بقيس الـ plasma
+
+71
+00:05:45,090 --> 00:05:50,210
+volume، أو مسافة الـ plasma، هذه المسافة أنا بقيسها و
+
+72
+00:05:50,210 --> 00:05:54,870
+بقسمها على الزمن، هذه المسافة يا بنات كانت مشغولة
+
+73
+00:05:54,870 --> 00:06:01,410
+بالـ RBC، لما كان الـ blood well mixed، يعني ما عملنه
+
+74
+00:06:01,410 --> 00:06:07,390
+mixing منيح، تمام؟ بمجرّد إنّه ترسب، يعني الـ RBCs
+
+75
+00:06:07,390 --> 00:06:10,110
+قطعت هذه المسافة كلّها لحتى صارت في الـ sediment
+
+76
+00:06:10,110 --> 00:06:15,450
+تمام؟ إذا أنا بدي أقيس هذه المسافة على الزمن، على
+
+77
+00:06:15,450 --> 00:06:18,110
+العكس من الـ hematocrite، الـ hematocrite أنا إيش كنت
+
+78
+00:06:18,110 --> 00:06:22,770
+أعمل؟ كنت أقيس نسبة الـ .. الـ RBCs، يعني، تمام؟
+
+79
+00:06:22,770 --> 00:06:26,090
+بالنسبة للـ whole blood volume، أعتبر إنها هي 100%،
+
+80
+00:06:26,090 --> 00:06:30,890
+و أشوف كم نسبة الـ RBCs منها بالمئة، ولكن في الـ ESR
+
+81
+00:06:30,890 --> 00:06:35,510
+لأ أنا بقيس المسافة للـ plasma على الـ .. الـ .. الزمن
+
+82
+00:06:35,510 --> 00:06:40,090
+اللي هي one hour، تمام؟ إذا هذه هي فكرة فحصنا لليوم
+
+83
+00:06:40,090 --> 00:06:43,890
+علشان لما نشرح كمان شوية، أو نبلش بالشرح، بعرض الـ
+
+84
+00:06:43,890 --> 00:06:50,430
+slides، نكون على الأقل متخيّلين إيش يعني فحص الـ ESR
+
+85
+00:06:51,000 --> 00:06:54,160
+Erythrocyte Sedimentation Rate، حكينا إنّي أنا
+
+86
+00:06:54,160 --> 00:06:59,500
+باستخدام أنابيب خاصة، بملأها بالـ blood اللي well
+
+87
+00:06:59,500 --> 00:07:04,280
+mixed، تمام، صار له mixing منيح مع anticoagulant، و
+
+88
+00:07:04,280 --> 00:07:10,380
+طبعاً بملأ هاي الأنابيب، و بجلسها بشكل عمودي، تمام،
+
+89
+00:07:10,380 --> 00:07:14,820
+على rack، طبعاً بيكون في خلفية الـ rack فيه تدريج، و
+
+90
+00:07:14,820 --> 00:07:18,800
+بعد ساعة، مرور ساعة من الزمن، أنا بقيس المسافة اللي
+
+91
+00:07:18,800 --> 00:07:22,600
+قطعتها الـ RBC، يعني مسافة البلازما، هاي فكرة الفحص
+
+92
+00:07:22,600 --> 00:07:27,020
+طبعاً الـ erythrocyte sedimentation rate، الـ erythrocyte
+
+93
+00:07:27,020 --> 00:07:32,740
+sedimentation rate، ESR is a non-specific measurement
+
+94
+00:07:32,740 --> 00:07:37,640
+used to detect and monitor an inflammatory response
+
+95
+00:07:37,640 --> 00:07:41,740
+to tissue injury and acute phase in which there is
+
+96
+00:07:41,740 --> 00:07:44,400
+a change in plasma concentration of several
+
+97
+00:07:44,400 --> 00:07:48,440
+proteins، ما معنى هذا الكلام؟ هلّأ الـ erythrocyte
+
+98
+00:07:48,440 --> 00:07:52,920
+sedimentation rate، فحص الـ ESR، هو عبارة عن فحص
+
+99
+00:07:52,920 --> 00:07:59,680
+يُستخدم، used to، يُستخدم ليه؟ لحتى أعمل detection and
+
+100
+00:07:59,680 --> 00:08:04,790
+monitoring، لمين؟ للـ inflammatory response، إذا كان
+
+101
+00:08:04,790 --> 00:08:09,670
+في .. في الجسم inflammation أو tissue injury أو
+
+102
+00:08:09,670 --> 00:08:14,210
+infection، تمام؟ ممكن أنا أعمل detection، تنبؤ عن
+
+103
+00:08:14,210 --> 00:08:18,230
+وجود هاي الـ .. الـ infection أو الـ inflammation أو
+
+104
+00:08:18,230 --> 00:08:21,950
+الـ tissue injury، ممكن أعمل monitoring، أشوف الوضع
+
+105
+00:08:21,950 --> 00:08:28,160
+بيتحسّن مع العلاج، أو بيزيد سوء مثلاً؟ تمام؟ بعمل
+
+106
+00:08:28,160 --> 00:08:33,600
+monitoring، مراقبة لحالة المريض من خلال فحص الـ ESR
+
+107
+00:08:33,600 --> 00:08:37,560
+المريض اللي بيكون عنده إما inflammation أو tissue
+
+108
+00:08:37,560 --> 00:08:43,100
+injury أو حتى infection، تمام؟ طيب ولكن هذا الفحص
+
+109
+00:08:43,100 --> 00:08:48,400
+is non-specific، يعني مثلاً إذا كانت نتيجة الـ ESR
+
+110
+00:08:48,400 --> 00:08:52,160
+عالية، بيكون يعني في عندي inflammation أو infection
+
+111
+00:08:52,160 --> 00:08:57,460
+أو tissue injury، طيب، خلاص أنا بعرف إنّه في
+
+112
+00:08:57,460 --> 00:09:02,840
+inflammation، لكن هل بـ .. يعني بإمكاني أحدّد وين الـ
+
+113
+00:09:02,840 --> 00:09:07,100
+infection أو وين الـ inflammation، في القلب، في الـ
+
+114
+00:09:07,100 --> 00:09:10,460
+kidney، في الـ liver، في الدم؟ وين بالظبط؟ أنا
+
+115
+00:09:10,460 --> 00:09:15,390
+ما بقدر أحدّدها، تمام؟ إذاً لا أستطيع تحديد مكان الـ
+
+116
+00:09:15,390 --> 00:09:18,150
+infection أو الـ inflammation، عشان هيك هو non-
+
+117
+00:09:18,150 --> 00:09:23,590
+specific، مش متخصص، فحص غير متخصص، ولكن هو فحص للـ
+
+118
+00:09:23,590 --> 00:09:28,910
+screening، تمام؟ للتشيك، لحتى أشيك، لحتى أكشف، أعمل
+
+119
+00:09:28,910 --> 00:09:33,510
+detection and monitoring للـ inflammation و الـ
+
+120
+00:09:33,510 --> 00:09:39,790
+tissue injury، و طبعاً يا بنات، لأنّه في حالة الـ
+
+121
+00:09:39,790 --> 00:09:42,330
+inflammation و الـ tissue injury و الـ infection
+
+122
+00:09:42,330 --> 00:09:48,010
+بيحدث، أو بيتم إنتاج بروتينات، أو بيزيد تركيز
+
+123
+00:09:48,010 --> 00:09:52,850
+بروتينات في الدم، في الـ plasma، بنسمّيها acute phase
+
+124
+00:09:52,850 --> 00:09:57,010
+protein، تمام؟ بنسمّيها الـ acute phase protein، طبعاً
+
+125
+00:09:57,010 --> 00:10:01,990
+هذه الـ acute phase protein بيلعب دور مهم جداً في
+
+126
+00:10:01,990 --> 00:10:08,580
+تسريع ترسيب الـ RBCs، طبعاً، فإذا وجود الـ acute phase
+
+127
+00:10:08,580 --> 00:10:12,880
+protein، دلالة على وجود acute phase of inflammation
+
+128
+00:10:12,880 --> 00:10:18,580
+أو tissue injury أو infection، إذاً ESR ما له بيكون
+
+129
+00:10:18,580 --> 00:10:24,300
+قيمته عالية، طيب this procedure very simply consists
+
+130
+00:10:24,300 --> 00:10:29,500
+of allowing a specific amount of blood to sit in
+
+131
+00:10:29,500 --> 00:10:33,000
+a vertical position for a period of time، يعني
+
+132
+00:10:33,000 --> 00:10:38,300
+وظيفته ببساطة، أو فكرة الفحص هذا ببساطة إنّه أنا
+
+133
+00:10:38,300 --> 00:10:44,660
+أخلي عينة الدم إنّها تكون موجودة بشكل عمودي لمدة
+
+134
+00:10:44,660 --> 00:10:50,100
+زمنية معيّنة، يعني one hour مثلاً، أو عادةً، يعني عادةً
+
+135
+00:10:50,190 --> 00:10:56,030
+نستخدمها يعني التوقيت one hour، و طبعاً أنا بقيس الـ
+
+136
+00:10:56,030 --> 00:10:58,890
+distance، اللي هي المسافة اللي قطعتها الـ RBCs، اللي
+
+137
+00:10:58,890 --> 00:11:04,210
+هي مسافة البلازما، تمام؟ بالـ millimeter per hour، إذاً
+
+138
+00:11:04,210 --> 00:11:10,140
+الوحدة القياس حتكون millimeter per hour، The ESR is
+
+139
+00:11:10,140 --> 00:11:15,740
+affected by three factors، الـ ESR هذا بيتأثّر بثلاث
+
+140
+00:11:15,740 --> 00:11:22,820
+عوامل، هناك ثلاثة players بيلعبوا دور مهم جداً في
+
+141
+00:11:22,820 --> 00:11:29,040
+نتيجة الـ ESR، أو قيمة الـ ESR، أوّل إيش؟ الـ erythrocyte
+
+142
+00:11:29,040 --> 00:11:32,220
+اللي .. اللي أصلاً مفعول بيه، أو اللي هي بتصير لها
+
+143
+00:11:32,220 --> 00:11:36,140
+sedimentation، أهم player عندي، أيضاً الـ plasma
+
+144
+00:11:36,140 --> 00:11:40,660
+composition، حكينا مكونات البلازما، البلازما بروتين
+
+145
+00:11:40,660 --> 00:11:46,140
+الـ acute phase protein اللي بتظهر، أو بيزيد تركيزها
+
+146
+00:11:46,140 --> 00:11:49,740
+في حالات الـ inflammation و الـ infection و الـ
+
+147
+00:11:49,740 --> 00:11:54,800
+tissue injury، طبعاً، هذه البروتينات موجودة في
+
+148
+00:11:54,800 --> 00:11:57,660
+البلازما، إذاً مكونات البلازما، البلازما
+
+149
+00:11:57,660 --> 00:12:05,100
+composition مهمّة جداً لحتى يعني نشوف كيف بتأثّر على
+
+150
+00:12:05,100 --> 00:12:08,700
+الـ ESR، أيضاً الـ mechanical و الـ technical factors،
+
+151
+00:12:08,700 --> 00:12:13,660
+عوامل يعني لها علاقة بشغلي، كيف أنا جمعت العينة، كيف
+
+152
+00:12:13,660 --> 00:12:16,780
+عملت لها mixing مع الـ anticoagulant، كيف اشتغلت
+
+153
+00:12:16,780 --> 00:12:20,300
+الفحص، البيئة و الظروف اللي اشتغلت فيها الفحص، هاي
+
+154
+00:12:20,300 --> 00:12:23,800
+ممكن تأثّر على النتيجة، راح نشوف كيف لاحقاً في الـ
+
+155
+00:12:23,800 --> 00:12:28,960
+slides التالية، خلينا نشوف أوّل player عندي اللي هي
+
+156
+00:12:28,960 --> 00:12:34,720
+الـ erythrocyte، erythrocyte is a factor of chief
+
+157
+00:12:34,720 --> 00:12:38,240
+importance in determining the distance that the
+
+158
+00:12:38,240 --> 00:12:43,320
+RBCs fall، تمام؟ بحكي لي إنّه الـ .. الـ .. الـ
+
+159
+00:12:43,320 --> 00:12:46,300
+erythrocyte، الـ erythrocyte يعني هي عبارة عن
+
+160
+00:12:46,300 --> 00:12:52,500
+عامل مهم جداً في التأثير على نتيجة الـ ESR، و لعله إنّه
+
+161
+00:12:52,500 --> 00:13:00,200
+أهم شيء بالـ RBCs هو حجمها، الـ size تبعها و الـ mass
+
+162
+00:13:00,200 --> 00:13:05,520
+تبعها، تمام؟ هو اللي بيتحكّم بمعدّل سقوطها، أو معدل
+
+163
+00:13:05,520 --> 00:13:12,210
+ترسيبها في الأنابيب، the larger the particle, the
+
+164
+00:13:12,210 --> 00:13:16,550
+faster its rate of fall، طبعاً يا بنات لو الـ slides
+
+165
+00:13:16,550 --> 00:13:21,390
+عندكم، المعلومة هي موجودة مختلفة، ياريت تنتبهوا لها و
+
+166
+00:13:21,390 --> 00:13:24,950
+تعدّلوها، the larger the particle, the faster its
+
+167
+00:13:24,950 --> 00:13:29,090
+rate of fall، طبعاً، يعني كل ما كبرت خلية الـ RBC
+
+168
+00:13:29,090 --> 00:13:36,670
+بيسرّع من سقوطها، من معدل سقوطها، تمام؟ طيب في عندي
+
+169
+00:13:36,670 --> 00:13:44,570
+أمراض ممكن تسبّب الـ RBCs size أكبر من الطبيعي، وفي
+
+170
+00:13:44,570 --> 00:13:49,910
+عندي أمراض ممكن تتسبّب إنّه الـ RBCs يكون حجمها أقلّ
+
+171
+00:13:49,910 --> 00:13:55,450
+من الطبيعي، فالأمراض اللي بتسبّب لي خلايا RBCs حجمها
+
+172
+00:13:55,450 --> 00:14:00,070
+أكبر من الطبيعي، يعني larger particle
+
+223
+00:17:49,190 --> 00:17:54,400
+بين الـ RBCs فيعني مدام صار في عندي crowding أو
+
+224
+00:17:54,400 --> 00:17:57,860
+تزاحم ما بين الـ RBC أو تقارب ما بينها خلينا نسمي
+
+225
+00:17:57,860 --> 00:18:01,920
+تقارب ما بينهم إذا صار في تجمعات من .. ما بين الـ
+
+226
+00:18:01,920 --> 00:18:08,360
+RBCs إذا طالما صار في عندي تجمعات من الـ RBCs يعني
+
+227
+00:18:08,360 --> 00:18:13,120
+صار في عندها .. عندي larger particle كبير حجمها
+
+228
+00:18:13,120 --> 00:18:19,150
+إذا تسقط تمام بيصير لها sedimentation طيب، هذا
+
+229
+00:18:19,150 --> 00:18:23,890
+الوضع الطبيعي احنا بنحكي، طبعا؟ طيب خلينا نشوف لو
+
+230
+00:18:23,890 --> 00:18:29,660
+.. لو في عندي certain disease لو في حالة certain
+
+231
+00:18:29,660 --> 00:18:34,720
+disease، الـ plasma protein concentration و may be
+
+232
+00:18:34,720 --> 00:18:39,900
+altered، تركيز الـ plasma protein هيتغير، هيزيد عندي
+
+233
+00:18:39,900 --> 00:18:44,760
+مين؟ هيزيد عندي الـ .. الـ acute phase protein في
+
+234
+00:18:44,760 --> 00:18:47,160
+حالات الـ infection و الـ inflammation و الـ tissue
+
+235
+00:18:47,160 --> 00:18:52,100
+injury، تمام؟ إذا هاي الـ acute phase protein اللي
+
+236
+00:18:52,100 --> 00:18:58,020
+زادت عن حدها، زادت على الحد الطبيعي، إيش هتعمل؟
+
+237
+00:18:58,020 --> 00:19:02,000
+هتعمل لي reduction في الـ negative charge أكثر و
+
+238
+00:19:02,000 --> 00:19:07,560
+هتعمل لي تجمعات أكثر و هتقلل الـ zeta potential اللي
+
+239
+00:19:07,560 --> 00:19:11,640
+ما بين الخلايا أكثر و تقرب الخلايا ما بينها و ما
+
+240
+00:19:11,640 --> 00:19:16,100
+بين بعضها أكثر، تمام؟ فبالتالي هذا إيش هيعمل لي؟
+
+241
+00:19:16,100 --> 00:19:21,820
+Larger mass، كتلة أكبر من الخلايا تسقط أسرع، إذا أنا
+
+242
+00:19:21,820 --> 00:19:27,260
+في عندي ESR أعلى، يعني أنا ترسيب عندي في .. بيصير
+
+243
+00:19:27,260 --> 00:19:31,540
+increase in the velocity، في سرعة الترسيب، تمام؟
+
+244
+00:19:31,540 --> 00:19:34,500
+increase sedimentation velocity أو increase
+
+245
+00:19:34,500 --> 00:19:39,600
+sedimentation rate، معدل الترسيب أشماله بيصير ..
+
+246
+00:19:39,600 --> 00:19:46,490
+بيصير أعلى، تمام؟ بيصير أعلى نتيجة إنه البروتينات
+
+247
+00:19:46,490 --> 00:19:53,110
+زادت، الـ acute phase protein فأدت إلى تقارب أكثر ما
+
+248
+00:19:53,110 --> 00:19:57,430
+بين الخلايا و تجمعات أكثر، هاي تجمعات الخلايا يا
+
+249
+00:19:57,430 --> 00:20:01,450
+بنات بسميها roll-off formation، إيش بسميها؟ roll
+
+250
+00:20:01,450 --> 00:20:04,790
+-off formation، ورح نشرح إيش يعني roll-off
+
+251
+00:20:04,790 --> 00:20:09,910
+formation خلال الـ slides القادمة، تمام؟ بس خليني
+
+252
+00:20:09,910 --> 00:20:18,350
+أشرح لكم على هاي الـ slide، خليني أشوف خلايا متباعدة
+
+253
+00:20:18,350 --> 00:20:24,190
+عن بعضها البعض مع عملات تجمعات، تمام؟ فيعني زي
+
+254
+00:20:24,190 --> 00:20:29,490
+السيارات كأنها بتقطع مسافة أو بسرعة متباعدة مع
+
+255
+00:20:29,490 --> 00:20:35,080
+بعضها البعض، تمام؟ تخيل .. تخيليها سيارات متباعدة
+
+256
+00:20:35,080 --> 00:20:40,240
+يعني هي زي الـ RBCs المتباعدة، طبعا؟ فمعدل سقوط هذه
+
+257
+00:20:40,240 --> 00:20:43,960
+الـ RBCs نتيجة إنها متباعدة يعني مافيش عندي ..
+
+258
+00:20:43,960 --> 00:20:50,500
+مافيش عندي تجمعات، طبعا؟ مافيش عندي تجمعات للـ
+
+259
+00:20:50,500 --> 00:20:54,400
+RBCs، إذن هل هتسقط بسرعة؟ لأ، يعني هت .. ممكن تاخد
+
+260
+00:20:54,400 --> 00:20:59,540
+الوضع الطبيعي للسقوط نتيجة التباعد الطبيعي ونتيجة
+
+261
+00:20:59,540 --> 00:21:03,980
+وجود الـ acute phase protein بالمعدل الطبيعي، تمام؟
+
+262
+00:21:03,980 --> 00:21:07,720
+زادت الـ acute phase protein يعني تقاربت الـ RBCs
+
+263
+00:21:07,720 --> 00:21:12,280
+أكثر، تمام؟ فصار في عندي، الـ .. الـ .. الـ .. زي
+
+264
+00:21:12,280 --> 00:21:15,500
+هنا السيارات مثلا صارت متقاربة من بعضها البعض
+
+265
+00:21:15,500 --> 00:21:19,860
+تمام؟ طيب فإذا طالما أنها تقاربت إذا صار في عندي
+
+266
+00:21:19,860 --> 00:21:25,530
+نسبة يعني .. معدل سقوطها على طريق ما، طريق يعني
+
+267
+00:21:25,530 --> 00:21:30,350
+نازل بنزول، هيكون أسرع، صح؟ تمام، نفس الفكرة هنا
+
+268
+00:21:30,350 --> 00:21:34,970
+طيب، لو كانت الخلايا متقاربة أكثر فأكثر فأكثر،
+
+269
+00:21:34,970 --> 00:21:38,610
+فتجمعت يعني، فهتكون زي كـ .. زي كـ .. زي كأنها قطار
+
+270
+00:21:38,610 --> 00:21:43,240
+ومسرع في النزول و زي .. هيك عمل لي تجمعات هذا
+
+271
+00:21:43,240 --> 00:21:48,340
+التجمعات أنا بسميها roll-off formation، تمام؟
+
+272
+00:21:48,340 --> 00:21:51,800
+بسميها roll-off formation، طب إيش يعني roll-off
+
+273
+00:21:51,800 --> 00:21:56,180
+formation؟ يعني إنه الخلايا الـ RBC بتكون متجمعة
+
+274
+00:21:56,180 --> 00:22:02,240
+على بعضها البعض بشكل like stack coins، إيش يعني like
+
+275
+00:22:02,240 --> 00:22:08,520
+stack coins؟ يعني زي القطع المعدنية اللي منزلقة
+
+276
+00:22:08,520 --> 00:22:13,020
+على بعضها البعض مثل القطع المعدنية اللي منزلقة على
+
+277
+00:22:13,020 --> 00:22:16,840
+بعضها البعض، تخيلي أنتِ عندك صفة شواكل زي هيك و جيتي
+
+278
+00:22:16,840 --> 00:22:20,880
+ده حرشتيهم و منزلقوا على بعضهم البعض، هذا بسميه شكل
+
+279
+00:22:20,880 --> 00:22:23,480
+الـ roll-off formation، نفس الشيء الخلايا بتاخد هذا
+
+280
+00:22:23,480 --> 00:22:29,020
+الشكل، خليني هنا مثلا أورجيكم إياه على الصور
+
+281
+00:22:29,020 --> 00:22:39,440
+لحتى تشوفوا كيف إيش يعني له low formation، تمام؟
+
+282
+00:22:39,440 --> 00:22:45,690
+هنشوف الصور هنا، Rouleau formation، هاي شايفين؟ خلايا
+
+283
+00:22:45,690 --> 00:22:50,130
+الـ RBCs تأخد شكل stacking coins، زي كأنها قطع
+
+284
+00:22:50,130 --> 00:22:53,110
+معدنية ومنزلقة على بعض البعض، زي صفة الشواكل اللي
+
+285
+00:22:53,110 --> 00:22:58,250
+أنتِ بتدحرجيهم تحت الـ microscope بتكون بهذا الشكل
+
+286
+00:22:58,250 --> 00:23:01,790
+شايفين؟ هاي هنا كمان أيضا تحت الـ microscope هذا
+
+287
+00:23:01,790 --> 00:23:05,350
+بسميه Rouleau formation، هذا Rouleau formation، هذا
+
+288
+00:23:05,350 --> 00:23:09,930
+Rouleau formation، تمام؟ إذن هذه صورة تقريبية، و هاي
+
+289
+00:23:09,930 --> 00:23:13,750
+صورة تحت الـ microscope له formation، زي الـ RBC's
+
+290
+00:23:13,750 --> 00:23:17,710
+منزلقة على بعضها البعض مثل الـ stack coins، تمام؟
+
+291
+00:23:17,710 --> 00:23:26,100
+like stack coins، طيب هيك بنكون شرحنا فكرة الفحص،
+
+292
+00:23:26,100 --> 00:23:29,580
+عرفنا إيش دور الاريثروسايت في الـ .. الـ
+
+293
+00:23:29,580 --> 00:23:34,980
+sedimentation، طبعا، حجمها ووجود البروتينة ..
+
+294
+00:23:34,980 --> 00:23:39,100
+وشحناتها، الـ negative charge اللي حواليها، التباعد
+
+295
+00:23:39,100 --> 00:23:45,670
+ما بينها، وجود البروتينات بنسبة معينة ممكن تقرب ما
+
+296
+00:23:45,670 --> 00:23:50,690
+بينها، يصير في عندي تجمعات، roll-off formation، يعني
+
+297
+00:23:50,690 --> 00:23:55,830
+صار في عندي larger particle، فهيسقط بشكل أسرع
+
+298
+00:23:55,830 --> 00:23:59,190
+وبالتالي هيصير في عندي sedimentation rate، هذا
+
+299
+00:23:59,190 --> 00:24:02,670
+بالوضع الطبيعي هيكون الـ sedimentation rate طبيعي
+
+300
+00:24:02,670 --> 00:24:05,630
+ونسبة أيضا البروتينات اللي ساهمت في التقارب ما بين
+
+301
+00:24:05,630 --> 00:24:09,210
+الـ RBC و الـ roll-off formation هتكون طبيعية، في حالة
+
+302
+00:24:09,210 --> 00:24:11,970
+disease، إذا أنا في عندي plasma protein
+
+303
+00:24:11,970 --> 00:24:15,830
+concentration عالي، يعني acute phase protein عالي
+
+304
+00:24:15,830 --> 00:24:19,950
+فبالتالي هيكون عندي .. هيصير في reduction في الـ
+
+305
+00:24:19,950 --> 00:24:23,010
+negative charge، هيكون في تقارب ما بين الـ RBCs
+
+306
+00:24:23,010 --> 00:24:27,370
+أكثر، rouleau formation أكثر، larger mass، increase
+
+307
+00:24:27,370 --> 00:24:31,190
+sedimentation rate، تمام؟ و أيضا في حالة الـ
+
+308
+00:24:31,190 --> 00:24:35,930
+agglutination يا بنات، تجلط الدم أو تجمع خلايا الدم
+
+309
+00:24:35,930 --> 00:24:40,930
+مع بعضها البعض و نتيجة تغير شكلها أو تغير سطحها
+
+310
+00:24:40,930 --> 00:24:47,190
+هذا أيضا ممكن إنه يعملي increase RBCs mass، يزود
+
+311
+00:24:47,190 --> 00:24:51,370
+من كتلة الـ RBCs، فيسرع سقوطها، يعني هلأ أنا طبعا لو
+
+312
+00:24:51,370 --> 00:24:57,390
+بدي أرمي يعني أسقط صخرة من علو ولا أسقط حجر صغير
+
+313
+00:24:57,390 --> 00:25:02,260
+الصخرة هتنزل أسرع نتيجة كتلتها أعلى، تمام؟ هاي هنا
+
+314
+00:25:02,260 --> 00:25:07,160
+بس الفكرة في الـ RBCs، larger particle، faster rate to
+
+315
+00:25:07,160 --> 00:25:14,450
+fall، طيب الـ macrocyte .. macro معناها كبير
+
+316
+00:25:14,450 --> 00:25:19,810
+حجمها كبير، very .. يعني large size،  خلية
+
+317
+00:25:19,810 --> 00:25:24,630
+الخلية اللي حجمها كبير tend to settle more rapidly
+
+318
+00:25:24,630 --> 00:25:29,350
+than .. mean than microcyte، micro يعني صغير و
+
+319
+00:25:29,350 --> 00:25:33,100
+cyte، خلية، إذا الخلايا اللي حجمها كبير نتيجة إن الـ
+
+320
+00:25:33,100 --> 00:25:36,380
+mass تبعتها كبيرة و الـ size تبعتها كبير، حكينا
+
+321
+00:25:36,380 --> 00:25:40,340
+أشماله fall أو settle أو sedimentation بيصير لها
+
+322
+00:25:40,340 --> 00:25:44,800
+sedimentation more rapidly أسرع من الـ microcyte
+
+323
+00:25:44,800 --> 00:25:51,020
+في حالة الـ anisocytosis و الـ poikilocytosis رح يصير
+
+324
+00:25:51,020 --> 00:25:57,480
+في عندي reduce للـ RBCs aggregation، فبالتالي هيقل
+
+325
+00:25:57,480 --> 00:26:02,980
+الـ ESR، إيش يعني أنيسوسايتوسز و بويكيلوسايتوسز؟
+
+326
+00:26:02,980 --> 00:26:06,340
+هلأ الانيسوسايتوسز يعني variation في الـ cell size
+
+327
+00:26:06,340 --> 00:26:12,240
+يعني تباين في حجم الخلايا، تمام؟ تباين في حجم
+
+328
+00:26:12,240 --> 00:26:16,740
+الخلايا، يعني الخلايا حجمها كبير، خلايا وسط، خلايا
+
+329
+00:26:16,740 --> 00:26:22,780
+صغيرة، هكذا يعني، طيب بويكيلوسايتوسز تباين في شكل
+
+330
+00:26:22,780 --> 00:26:26,680
+الخلايا، في shape للخلايا، يعني عندي خلايا شكلها
+
+331
+00:26:26,680 --> 00:26:30,180
+الشكل الطبيعي اللي هي biconcave أو discocyte، في
+
+332
+00:26:30,180 --> 00:26:34,360
+عندي خلايا spherocyte شكلها كروي، في خلايا شكلها
+
+333
+00:26:34,360 --> 00:26:39,280
+شكل الـ sickle cell، منجلية، تمام؟ فهذا التباين في
+
+334
+00:26:39,280 --> 00:26:45,000
+شكل الخلايا بسميه بويكيلوسايتوسز، طيب، هلأ تجمعات
+
+335
+00:26:45,000 --> 00:26:49,720
+الخلايا لازم تكون الخلايا .. يعني لحتى تحدث هاي
+
+336
+00:26:49,720 --> 00:26:52,200
+التجمعات، لحتى يحدث الـ roll-off formation أو الـ
+
+337
+00:26:52,200 --> 00:26:56,780
+RBC aggregation، هذا لازم إن الخلايا تكون uniform،
+
+338
+00:26:56,780 --> 00:27:00,540
+منتظمة الشكل والحجم عشان إنها تتجمع مع بعضها
+
+339
+00:27:00,540 --> 00:27:03,520
+البعض، تلتصق زي الـ stack coins زي ما شوفنا في
+
+340
+00:27:03,520 --> 00:27:09,220
+الصورة من شوي و تسقط مع بعضها البعض، كلن يعني يتجمع
+
+341
+00:27:09,220 --> 00:27:14,320
+على شكلها، طبعا الـ RBCs لازم تكون يعني بتشبه بعضها
+
+342
+00:27:14,320 --> 00:27:19,520
+بالشكل و الحجم علشان إيش أشماله إنه يعني يصير لها
+
+343
+00:27:19,520 --> 00:27:23,300
+sedimentation مع بعض، تصير لها aggregation وبعدها
+
+344
+00:27:23,300 --> 00:27:28,070
+sedimentation مع بعض، إحنا مثلا لما بنصادق ناس من
+
+345
+00:27:28,070 --> 00:27:31,610
+نصادقهم بيشبهونا، مش شرط بالشكل أو بالحجم، ممكن
+
+346
+00:27:31,610 --> 00:27:37,590
+يكونوا بيشبهونا بأشياء، تمام؟ فإنه يعني إحنا بيكون
+
+347
+00:27:37,590 --> 00:27:42,290
+في تواؤم بينهم، على نفس الفكرة الـ RBCs لازم إنه
+
+348
+00:27:42,290 --> 00:27:45,810
+علشان يصير لها aggregation و rouleau formation تكون ..
+
+349
+00:27:45,810 --> 00:27:52,010
+تكون على الأقل منتظمة الشكل و الحجم، طبعا طيب، لكن
+
+350
+00:27:52,010 --> 00:27:55,210
+إذا كان في عندي تباين في الحجم أو تباين في الشكل
+
+351
+00:27:55,210 --> 00:28:00,630
+هذا راح يقود إلى inhibition أو reduce the ability
+
+352
+00:28:00,630 --> 00:28:06,630
+of RBCs، يقلل من قدرة خلايا الـ RBCs على إنها تعمل
+
+353
+00:28:06,630 --> 00:28:12,790
+aggregation، تعمل larger particle، ويحصل، فخلايا الـ
+
+354
+00:28:12,790 --> 00:28:16,450
+RBCs راح تسقط يعني على الأغلب فرادى أو هي و هي
+
+355
+00:28:16,450 --> 00:28:22,070
+تجمعات قليلة صغيرة جدا، فهذا يقلل الـ ESR طبعا؟
+
+356
+00:28:22,070 --> 00:28:23,710
+معدل الترسيب
+
+357
+00:28:26,070 --> 00:28:30,630
+طيب RBCs، هنا بيشرح لي إيش معنى كلمة anisocytosis
+
+358
+00:28:30,630 --> 00:28:33,650
+and poikilocytosis، بتقلل الـ aggregation و الـ rouleau
+
+359
+00:28:33,650 --> 00:28:37,930
+formation و بتقلل الـ ESR، بيحكي لك الـ RBCs should
+
+360
+00:28:37,930 --> 00:28:41,730
+show an alteration in shape، يعني شكلها يعني متغير
+
+361
+00:28:41,730 --> 00:28:46,600
+زي الـ sickle cell، خلايا المنجلية و الكروية طبعا
+
+362
+00:28:46,600 --> 00:28:51,200
+ما .. ما .. يعني لا تتمكن أو لا تفتقد القدرة على
+
+363
+00:28:51,200 --> 00:28:54,740
+إنها تعمل لي aggregation أو rouleau formation، فبالتالي
+
+364
+00:28:54,740 --> 00:28:58,240
+الـ erythrocyte sedimentation rate أشماله بيكون
+
+365
+00:28:58,240 --> 00:29:02,580
+decreased، في حالة الانيسوسايتوسز و البويكيلوسايتوسز
+
+366
+00:29:04,000 --> 00:29:09,440
+تمام، في حالة الـ severe anemia، anemia فقر دم يعني
+
+367
+00:29:09,440 --> 00:29:14,420
+في .. severe، كتير .. يعني كتير متقدمة .. متقدمة
+
+368
+00:29:14,420 --> 00:29:18,760
+الحالة، يعني كتير anemia، مستوى الـ anemia كتير،
+
+369
+00:29:18,760 --> 00:29:24,120
+تمام؟ طيب، the ESR is markedly elevated، بيكون
+
+370
+00:29:24,120 --> 00:29:28,840
+عالي، ليش؟ لأن anemia يعني نقص hemoglobin، يعني
+
+371
+00:29:28,840 --> 00:29:35,930
+نقص RBCs، red blood cells، قليلة بالنسبة للبلازما، فلما
+
+372
+00:29:35,930 --> 00:29:40,270
+بتسقط أشماله أول حاجة ما بدهاش وقت لتسقط لإن عددها
+
+373
+00:29:40,270 --> 00:29:47,330
+قليل، ثاني شيء لإن نسبة البلازما بتأخذ حيز أكبر من
+
+374
+00:29:47,330 --> 00:29:54,340
+الخلايا، فهذا رح يقود إلى إن الـ ESR يزيد، إذا الـ
+
+375
+00:29:54,340 --> 00:29:58,380
+concentration of RBCs قليل في حالة الانيميا، تركيز
+
+376
+00:29:58,380 --> 00:30:02,520
+الخلايا، عدد الخلايا قليل في الـ plasma، فالـ
+
+377
+00:30:02,520 --> 00:30:05,860
+aggregation و الـ rouleau formation هيزيد، تمام؟
+
+378
+00:30:06,580 --> 00:30:12,060
+بالإضافة يعني هتسقط بسرعة لإنه عددها قليل و لإنه
+
+379
+00:30:12,060 --> 00:30:16,540
+تجمعها هيكون بسرعة و لإنه نسبة البلازما بالنسبة
+
+380
+00:30:16,540 --> 00:30:22,060
+للخلايا أعلى، فهذه أسباب كافية لإنه تخلي لي الـ ESR
+
+381
+00:30:22,060 --> 00:30:26,840
+markedly elevated، طبعا؟ طيب في حالة الـ
+
+382
+00:30:26,840 --> 00:30:33,000
+polycythemia، ESR is generally decreased، طبعا في
+
+383
+00:30:33,000 --> 00:30:36,860
+حالة البوليسيثيميا، الـ ESR is generally decreased
+
+384
+00:30:36,860 --> 00:30:41,440
+بيقل الـ ESR، أول شيء طبعا إحنا حكينا إنه
+
+385
+00:30:41,440 --> 00:30:46,120
+البوليسيثيميا يعني زيادة في خلايا الدم، الـ RBCs
+
+386
+00:30:46,120 --> 00:30:49,500
+طبعا، بنعكس الـ concentration هنا في حالة
+
+387
+00:30:49,500 --> 00:30:54,410
+البوليسيثيميا، إيش ماله هيكون increase، تمام؟ يعني الـ
+
+388
+00:30:54,410 --> 00:30:56,950
+aggregation و الـ rouleau formation أشماله هيكون
+
+389
+00:30:56,950 --> 00:31:00,350
+decrease، خلايا يعني بدها وقت لتتجمع مع بعض على
+
+390
+00:31:00,350 --> 00:31:04,550
+بعض، أو بدها وقت لتسقط لإن عددها كبير، تمام؟ و
+
+391
+00:31:04,550 --> 00:31:08,430
+أيضا في حالة البوليسايتيميا إحنا حكينا إنه نوع من
+
+392
+00:31:08,430 --> 00:31:13,410
+أنواعه أحيانا بيكون cancer، تمام؟ خلية الأم للـ
+
+393
+00:31:13,410 --> 00:31:17,790
+RBCs في الـ bone marrow بيصير فيها mutation
+
+445
+00:35:22,960 --> 00:35:29,850
+طبعًا طيب، فبالتالي although بالرغم من أن زيادة ال
+
+446
+00:35:29,850 --> 00:35:33,650
+plasma viscosity will tend to inhibit the fall of
+
+447
+00:35:33,650 --> 00:35:37,490
+RBCs, and the increase in plasma protein are
+
+448
+00:35:37,490 --> 00:35:41,170
+generally those which cause low and aggregation of
+
+449
+00:35:41,170 --> 00:35:44,690
+RBCs, which affect the ESR more greatly than the
+
+450
+00:35:44,690 --> 00:35:48,930
+increase in plasma viscosity. هلأ صح إن زيادة
+
+451
+00:35:48,930 --> 00:35:54,830
+البروتينات في البلازما ممكن إنها تزيد لزوجة
+
+452
+00:35:54,830 --> 00:36:01,250
+البلازمة، تمام؟ ولكن ممكن إنها تزيد لزوجة البلازمة
+
+453
+00:36:01,250 --> 00:36:09,650
+ولكن يعني .. يعني بغض النظر إنها لو زادت لزوجة
+
+454
+00:36:09,650 --> 00:36:13,650
+البلازمة، فبالتالي يعني إحنا عارفين إن إذا زادت
+
+455
+00:36:13,650 --> 00:36:17,950
+اللزوجة هي .. هي .. هي .. هيعمل إعاقة أو تبطيء
+
+456
+00:36:17,950 --> 00:36:24,200
+لسقوط خلايا الدم، تمام؟ طيب، هل هذه المعلومة
+
+457
+00:36:24,200 --> 00:36:27,500
+متناقدة مع المعلومة السابقة؟ آه، هي متناقدة
+
+458
+00:36:27,500 --> 00:36:31,920
+الصراحة، أنه إحنا .. طب stop يعني، إحنا هلأ بدنا
+
+459
+00:36:31,920 --> 00:36:35,200
+نعرف الـ plasma protein أو الـ acute phase protein هي
+
+460
+00:36:35,200 --> 00:36:39,680
+بتزود لزوجة، فبتقلل من معدل سقوط الـ RBCs، يعني
+
+461
+00:36:39,680 --> 00:36:44,180
+بتقلل الـ ESR، و اللي هي بتعمل role of formation و
+
+462
+00:36:44,180 --> 00:36:49,520
+aggregation، و بالتالي بتزود من تجمع الـ RBCs، فبتسرع
+
+463
+00:36:49,520 --> 00:36:54,340
+سقوطها. شوفوا معايا، هلأ الـ acute phase protein بتعمل
+
+464
+00:36:54,340 --> 00:36:59,180
+الشغلتين، ولكن كفة .. الكفة تبع الـ roll-off
+
+465
+00:36:59,180 --> 00:37:04,780
+formation و الـ aggregation بترجح أكتر، تمام؟ بتكون
+
+466
+00:37:04,780 --> 00:37:10,320
+تأثيرها أكبر، ما طالما إن هي تأثيرها في اتجاه الـ
+
+467
+00:37:10,320 --> 00:37:15,160
+roll-off and aggregation of RBCs أكبر، تمام؟
+
+468
+00:37:15,160 --> 00:37:22,040
+معناها الترسيب هيكون أسرع. طبعًا؟ طيب و .. لكن في
+
+469
+00:37:22,040 --> 00:37:26,560
+عندي بروتين اسمه الـ albumin طبعًا؟ هذا البروتين
+
+470
+00:37:26,560 --> 00:37:31,360
+تقريبا هو أعلى تركيز البروتين موجود في الدم، يعني
+
+471
+00:37:31,360 --> 00:37:34,920
+موجود و بيتصنع بنسبة معينة، هو أعلى بروتين موجود في
+
+472
+00:37:34,920 --> 00:37:41,420
+الدم طبعًا؟ هذا الـ protein هو اللي بيعطي الدم قوامه،
+
+473
+00:37:41,420 --> 00:37:46,720
+أنه هو يعني .. يعني أكتر لزوجة قليلًا من الميه،
+
+474
+00:37:46,720 --> 00:37:50,420
+تمام؟ هو اللي .. هو اللي مخلي الدم ما يصيرش ميه يا
+
+475
+00:37:50,420 --> 00:37:55,560
+بنات، هاي معناها بالعربي يعني هيك الـ albumin، تمام؟
+
+476
+00:37:55,560 --> 00:38:01,100
+لو زاد الـ albumin هذا نتيجة مرض معين، تمام؟ يعني
+
+477
+00:38:01,100 --> 00:38:06,120
+زادت لزوجة الدم، هنا شايفين الـ albumin وين بيلعب؟ بيلعب
+
+478
+00:38:06,120 --> 00:38:10,540
+أكتر على كفة مين؟ ع كفة اللزوجة، وليس ع كفة الـ roll
+
+479
+00:38:10,540 --> 00:38:14,440
+off formation and aggregation طبعًا؟ فإذا الـ albumin
+
+480
+00:38:14,440 --> 00:38:20,660
+إذا زاد، بزيد اللزوجة، فبيقلل الإياسار طبعًا؟ إذا
+
+481
+00:38:20,660 --> 00:38:26,480
+خلينا نلخص هلأ الـ protein بشكل عام، زيادة تركيزها
+
+482
+00:38:26,480 --> 00:38:31,470
+في الدم طبعًا؟ زيادة تركيزها في الدم ممكن تعملي
+
+483
+00:38:31,470 --> 00:38:34,510
+زيادة في الـ roll off formation و الـ aggregation،
+
+484
+00:38:34,510 --> 00:38:38,370
+فبالتالي بتسرع من سقوط الـ RBCs، فبالتالي بتسرع من
+
+485
+00:38:38,370 --> 00:38:45,470
+الـ ESR، تمام، increased ESR، أو أو ممكن لِبروتينات
+
+486
+00:38:45,470 --> 00:38:49,770
+high تعمل increase في الـ viscosity، يعني زيادة في
+
+487
+00:38:49,770 --> 00:38:55,790
+اللزوجة للبلازمة، فبالتالي بتعمل إعاقة لسقوط الـ RBCs،
+
+488
+00:38:55,790 --> 00:39:01,690
+فبتقلل الـ ESR. طيب، الإثنين موجودات، والإحتمالين
+
+489
+00:39:01,690 --> 00:39:07,050
+موجودين، طبعًا، ولكن الـ acute phase protein بتشتغل
+
+490
+00:39:07,050 --> 00:39:11,770
+باتجاه الـ roll off formation و الـ aggregation أكثر،
+
+491
+00:39:11,770 --> 00:39:14,870
+طبعًا، فبالتالي عشان هيك الـ acute phase protein
+
+492
+00:39:14,870 --> 00:39:21,290
+بتسرع الـ ESR، ولكن الـ albumin زيادته هيشتغل باتجاه
+
+493
+00:39:21,290 --> 00:39:26,330
+زيادة الـ viscosity أكثر. تمام؟ فبالتالي زيادة الـ ..
+
+494
+00:39:26,330 --> 00:39:31,610
+الـ albumin، يعني increased of viscosity، يعني هيعمل لي
+
+495
+00:39:31,610 --> 00:39:37,150
+inhibition للـ fall of RBCs، يعني هيعطيني lower ESR،
+
+496
+00:39:37,150 --> 00:39:44,230
+أو decreased ESR. تمام؟ إذًا هذا الـ .. الـ .. يعني
+
+497
+00:39:44,230 --> 00:39:48,370
+دور الـ plasma protein في الـ ESR. طيب، في عندي أيضًا
+
+498
+00:39:48,370 --> 00:39:52,750
+mechanical and technical factors، عوامل تقنية أو
+
+499
+00:39:52,750 --> 00:40:00,010
+ميكانيكية لحتى بتأثر على قيمة الـ ESR في شغلنا
+
+500
+00:40:00,010 --> 00:40:06,370
+أثناء شغلنا، و هاي العوامل بتأثر على قيمة الـ ESR.
+
+501
+00:40:06,960 --> 00:40:10,000
+يعني كيف أنا اشتغلت العينة، كيف سحبتها، كيف
+
+502
+00:40:10,000 --> 00:40:12,480
+عملت لها mixing مع الـ anticoagulant، نوع الـ
+
+503
+00:40:12,480 --> 00:40:16,520
+anticoagulant اللي استخدمته، هل خليت الـ .. الـ tube
+
+504
+00:40:16,520 --> 00:40:20,260
+vertical أو مش vertical، في درجة الحرارة، هذه كلها
+
+505
+00:40:20,260 --> 00:40:23,360
+عوامل mechanical and technical، عوامل تقنية
+
+506
+00:40:23,360 --> 00:40:29,410
+وميكانيكية بتؤثر على النتيجة. طبعًا مثال عليها إحنا
+
+507
+00:40:29,410 --> 00:40:34,210
+حكينا أن الـ ESR should be exactly perpendicular،
+
+508
+00:40:34,210 --> 00:40:39,510
+عمودية، تصنع درجة تسعين درجة، تمام؟ تصنع .. تصنع
+
+509
+00:40:39,510 --> 00:40:44,710
+زاوية تسعين درجة مع الـ bench، تمام؟ فبالتالي
+
+510
+00:40:44,710 --> 00:40:49,850
+الإمالة، الـ tilt .. الـ tilt يعني الإمالة، الإمالة
+
+511
+00:40:49,850 --> 00:40:54,470
+في هذه الـ tube تلاتين درجة هتعطيني error تلاتين في
+
+512
+00:40:54,470 --> 00:41:00,470
+المئة. طبعًا الـ error 30% يعني 30% زيادة في قيمة الـ
+
+513
+00:41:00,470 --> 00:41:06,690
+ESR، لأن إمالة الـ tube بتزود الـ ESR، بتزود القيمة
+
+514
+00:41:06,690 --> 00:41:11,570
+ليش طيب بتزود القيمة؟ الـ tube مائل، يعني عندي سطح
+
+515
+00:41:11,570 --> 00:41:17,770
+مساحة سطح، في .. لا، الـ roll off formation أكبر، يعني
+
+516
+00:41:17,770 --> 00:41:24,480
+roll off formation أكثر، يعني ESR أسرع و أعلى. تمام؟
+
+517
+00:41:24,480 --> 00:41:27,720
+إذا هذا هيأثر على النتائج، أنا عندي قيم عالية، يعني هي ممكن أشك أن الشخص هذا عنده
+
+518
+00:41:27,720 --> 00:41:29,860
+infection, inflammation, tissue injury، فإذا .. و
+
+519
+00:41:29,860 --> 00:41:33,660
+النتيجة ممكن تكون falsely elevated، يعني هي مرتفعة
+
+520
+00:41:33,660 --> 00:41:37,460
+بالغلط، نتيجة a tilting، نتيجة إيه معناه في
+
+521
+00:41:37,460 --> 00:41:40,820
+الإتيوب؟ طيب also the rack holding the tube، الـ rack
+
+522
+00:41:40,820 --> 00:41:45,870
+اللي أنا ببدأ أحط عليه القنابيب تبعتي shouldn't be
+
+523
+00:41:45,870 --> 00:41:49,270
+subject to any movement or vibration، مش لازم يتعرض
+
+524
+00:41:49,270 --> 00:41:54,910
+لي أي اهتزازات أو حركة، هذا الـ bench. تمام؟ طيب الـ
+
+525
+00:41:54,910 --> 00:42:01,260
+bench اللي أنا بدي أحط عليه الـ racks لازم يكون ثابت،
+
+526
+00:42:01,260 --> 00:42:03,760
+لأ ما فيش فيه عندي vibration، اهتزازات أو حركة، يعني
+
+527
+00:42:03,760 --> 00:42:08,780
+مثلاً أنا بدي أحط الـ rack تبع القنابيب تبعتي بعيد عن
+
+528
+00:42:08,780 --> 00:42:11,760
+جهاز الـ centrifuge، بعيد عن التلاجة، بعيد عن أي جهاز
+
+529
+00:42:11,760 --> 00:42:15,820
+مشغل و ممكن يعملي ذبذبات أو اهتزازات أو vibration،
+
+530
+00:42:15,820 --> 00:42:20,400
+للـ bench اللي محطوط عليه القنابيب، تمام؟ حتى ما
+
+531
+00:42:20,400 --> 00:42:24,080
+تهتز القنابيب، فبالتالي يتأثر الـ sedimentation. تمام،
+
+533
+00:42:29,090 --> 00:42:33,530
+هلأ minor everyday variation in room temperature
+
+534
+00:42:33,530 --> 00:42:40,420
+don't significantly affect the ESR. تغير قليل في
+
+535
+00:42:40,420 --> 00:42:45,800
+الـ ESR .. في درجة الحرارة ما بيأثر على الـ ESR.
+
+536
+00:42:45,800 --> 00:42:48,980
+اليوم كانت درجة الحرارة تلاتين، مبارح تسعة و
+
+537
+00:42:48,980 --> 00:42:52,960
+عشرين، بكرا واحد و تلاتين، لو أنا جبت عينة من شخص و
+
+538
+00:42:52,960 --> 00:42:56,380
+اشتغلتها على التلت أيام هذه، في اختلاف درجات
+
+539
+00:42:56,380 --> 00:43:01,480
+الحرارة، في الـ minor variation هذا ممكن يعني نتيجة
+
+540
+00:43:01,480 --> 00:43:07,400
+الـ ESR تكون نفسها أو فيها تغير بسيط جدًا، ما بيأثر
+
+541
+00:43:07,400 --> 00:43:10,960
+يعني don't significantly affect ESR، يعني ما بيغير
+
+542
+00:43:10,960 --> 00:43:15,380
+كتير على نتيجة الـ ESR، لكن large changes in
+
+543
+00:43:15,380 --> 00:43:20,120
+temperature، يعني مثلاً تغير من عشرين لتلاتين، من
+
+544
+00:43:20,120 --> 00:43:24,140
+خمسة عشر لتلاتين، يعني تغير كبير في درجة الحرارة،
+
+545
+00:43:24,140 --> 00:43:28,140
+فهذا ممكن يأثر على نتيجة الـ ESR. الـ sedimentation
+
+546
+00:43:28,140 --> 00:43:31,960
+rate increases as temperature increases، كل ما زاد
+
+547
+00:43:31,960 --> 00:43:37,120
+.. كل ما زادت درجة الحرارة بتزيد أو بيزيد قيمة الـ
+
+548
+00:43:37,120 --> 00:43:43,120
+ESR. the length and inner diameter of ESR tube also
+
+549
+00:43:43,120 --> 00:43:47,280
+affect the final test result. كمان طول و القطر
+
+550
+00:43:47,280 --> 00:43:52,370
+الداخلي للـ ESR tube بيأثر على الـ test result. إحنا
+
+551
+00:43:52,370 --> 00:43:57,570
+حكينا أنابيب خاصة، تتذكروا حكينا أنابيب خاصة لفحص الـ
+
+552
+00:43:57,570 --> 00:44:01,770
+ESR، كمان شوية راح نستعرضهم، راح نشوفهم، تمام؟ وهي
+
+553
+00:44:01,770 --> 00:44:07,050
+هم موجودين، هم في الخلفية. طيب، فالـ ESR tube، أنابيب الـ
+
+554
+00:44:07,050 --> 00:44:14,850
+ESR، كل ما كانت أضيق، تمام؟ يعني أرفع الـ .. الـ .. الـ
+
+555
+00:44:14,850 --> 00:44:17,750
+bore، الـ internal bore .. الـ bore تبعها قليل أو
+
+556
+00:44:17,750 --> 00:44:23,180
+صغيرة، هتعطيني lower sedimentation rate، lower ESR
+
+557
+00:44:23,180 --> 00:44:29,780
+result، ليش؟ أنبوب دقيق يعني أو .. أو رفيع، ضيق،
+
+558
+00:44:29,780 --> 00:44:34,320
+معناه ما فيش عندي حكينا مساحة سطح كافية لـ الـ role
+
+559
+00:44:34,320 --> 00:44:40,300
+of formation و الـ RBCs aggregation، فبالتالي الـ ESR
+
+560
+00:44:40,300 --> 00:44:40,780
+هيقل.
+
+561
+00:44:43,650 --> 00:44:47,990
+طيب، إذا فائدة significance of the test، أو
+
+562
+00:44:47,990 --> 00:44:52,790
+significance of the ESR، فائدة الفحص تبعي، حكينا إن
+
+563
+00:44:52,790 --> 00:44:58,330
+هو بيمثل الـ ESR، represent a non-specific response
+
+564
+00:44:58,330 --> 00:45:01,910
+to tissue damage, inflammation, infection، وجود
+
+565
+00:45:01,910 --> 00:45:08,190
+disease معين، وما بيأثر على أو ما بيدل أو ما بيدلل
+
+566
+00:45:08,190 --> 00:45:11,710
+على الـ severity تبعه، but not its severity، يعني
+
+567
+00:45:11,710 --> 00:45:16,900
+مثلاً بيدل لي على وجود infection معينة، زي التهابات
+
+568
+00:45:16,900 --> 00:45:22,040
+المفاصل، أو inflammation معينة، سوري، زي التهابات
+
+569
+00:45:22,040 --> 00:45:24,920
+المفاصل، infection معينة زي الـ tuberculosis، مرض
+
+570
+00:45:24,920 --> 00:45:30,940
+السل، طن خلل في أعضاء معينة، failure في أعضاء، tissue
+
+571
+00:45:30,940 --> 00:45:36,910
+injury، يعني هذه كلها ممكن يدل لي عليها، ولكن non-
+
+572
+00:45:36,910 --> 00:45:40,510
+specific، حكينا، طبعًا، يعني أنا ما بعرف وين الـ
+
+573
+00:45:40,510 --> 00:45:43,990
+infection هاي، وين الـ inflammation هاي، حادثة، أو الـ
+
+574
+00:45:43,990 --> 00:45:48,530
+tissue injury، أنا بعرف إنه فيه، بعمل detection and
+
+575
+00:45:48,530 --> 00:45:51,590
+monitoring لـ الـ tissue damage و الـ inflammation و
+
+576
+00:45:51,590 --> 00:45:54,790
+الـ infection، ولكن بيكون non-specific، يعني ما بعرف
+
+577
+00:45:54,790 --> 00:45:59,170
+وينها، و ما بقدر أميز الـ severity تبعها. it's
+
+578
+00:45:59,170 --> 00:46:02,610
+primarily reflected changes in plasma protein، طبعًا
+
+579
+00:46:02,610 --> 00:46:06,350
+هو بيتأثر أكتر شيء في الـ plasma protein اللي هي
+
+580
+00:46:06,350 --> 00:46:11,310
+عبارة اللي بتكون يعني اللي بتظهر أو ممكن تزيد أو
+
+581
+00:46:11,310 --> 00:46:15,190
+يتأثر تركيزها في حالات الـ acute و الـ chronic
+
+582
+00:46:15,190 --> 00:46:18,590
+infection، في حالات الـ tumor، في حالات الـ
+
+583
+00:46:18,590 --> 00:46:21,710
+degenerative disease، يعني الأمراض الناتجة عن organ
+
+584
+00:46:21,710 --> 00:46:25,790
+failure. طبعًا هذه كلها بتأثر الـ plasma protein،
+
+585
+00:46:25,790 --> 00:46:29,250
+فبالتالي هيتأثر الـ ESR، و ممكن يكشف لي عنها، نعمل ..
+
+586
+00:46:29,250 --> 00:46:33,350
+نعمل detection لها، و أيضًا حكينا بيعمل لي
+
+587
+00:46:33,350 --> 00:46:38,530
+monitoring طبعًا، يعني follow up لـ الـ progress of
+
+588
+00:46:38,530 --> 00:46:43,530
+certain disease، يعني ممكن يعطيني .. يعني أشوف الـ
+
+589
+00:46:43,530 --> 00:46:48,860
+.. يعني أراقب وضع المريض، بيتحسن ولا ما بيتحسن؟ بيتحسن
+
+590
+00:46:48,860 --> 00:46:53,940
+ولا بيزيد سوء؟ تمام؟ فهذا مثلاً شخص مصاب بمرض السل،
+
+591
+00:46:53,940 --> 00:46:57,080
+أو بمرض الـ Rheumatoid Arthritis، التهاب المفاصل،
+
+592
+00:46:58,070 --> 00:47:02,650
+طبعًا، فممكن يعني إذا أعطينا .. يعني الـ ESR عملنا له
+
+593
+00:47:02,650 --> 00:47:06,650
+يا كان عالي نتيجة inflammation، عملنا له specific
+
+594
+00:47:06,650 --> 00:47:11,510
+test، فحوصات متخصصة أكثر، فكشفت عن وجود إما
+
+595
+00:47:11,510 --> 00:47:16,110
+tuberculosis، يعني سل، عدوى يعني infection، هذه طبعًا
+
+596
+00:47:16,110 --> 00:47:20,350
+بكتيريا بتسبب مرض السل طبعًا، أو rheumatoid
+
+597
+00:47:20,350 --> 00:47:25,140
+arthritis، التهابات المفاصل، ده مرض مناعي. طبعًا طيب
+
+598
+00:47:25,140 --> 00:47:29,380
+فاكتشفنا في الفحوصات المتخصصة أنه عنده إما
+
+599
+00:47:29,380 --> 00:47:33,320
+tuberculosis أو rheumatoid arthritis، الطبيب هيعطي
+
+600
+00:47:33,320 --> 00:47:39,100
+المريض علاجه ممكن لحتى نشوف استجابته، أو نشوف حالته
+
+601
+00:47:39,100 --> 00:47:44,960
+بتتحسن أو بتسوء، ممكن أشتغل الـ ESR مرة ثانية، أشوفه
+
+602
+00:47:44,960 --> 00:47:51,500
+أقارنه بالنتيجة السابقة، زاد أو نقص. لكن يعني أنا ما
+
+603
+00:47:51,500 --> 00:47:57,320
+بحدد الـ severity، يعني مدى سوء الحالة، من أول فحص،
+
+604
+00:47:57,320 --> 00:48:03,400
+طبعًا، ممكن بعد العلاج أشوف الفحص الثاني و أقارنه
+
+605
+00:48:03,400 --> 00:48:07,780
+بالأول عشان أحكم هو إتحسن ولا ما إتحسن، لكن من فحص
+
+606
+00:48:07,780 --> 00:48:11,720
+واحد لأول مرة، يعني ممكن يدل لي إنه في infection أو
+
+607
+00:48:11,720 --> 00:48:17,360
+inflammation، هو المريض وضعه تعبان على الآخر ولا في
+
+608
+00:48:17,360 --> 00:48:23,180
+بدايات المرض، ممكن نلحّق ولا لأ، هذه أمور لا يمكن أن
+
+609
+00:48:23,180 --> 00:48:26,660
+إحنا نعرفها من خلال الـ ESR اللي هو فحص لحاله، يعني
+
+610
+00:48:26,660 --> 00:48:32,500
+أو أنه نتيجة واحدة، أو حتى فحص لحاله، بده other tests.
+
+611
+00:48:32,500 --> 00:48:35,100
+طيب.
+
+612
+00:48:36,180 --> 00:48:41,380
+An elevated ESR may be found in، زيادة الـ ESR ممكن
+
+613
+00:48:41,380 --> 00:48:45,220
+تكون موجودة في حالات، هلأ بتشوفوا الحالات هذه، هتكون
+
+614
+00:48:45,220 --> 00:48:47,920
+إما عبارة عن inflammation أو infection أو tissue
+
+615
+00:48:47,920 --> 00:48:51,940
+injury. خلينا نشوف acute and chronic infection،
+
+616
+00:48:51,940 --> 
+
+667
+00:52:47,320 --> 00:52:52,580
+بدون ما يفهم وين الـ... وين الخلل تمام؟ طيب بدون ما
+
+668
+00:52:52,580 --> 00:52:56,900
+يشوف أنَّ المشكلة في القلب مش في التصنيع فبيبدأ
+
+669
+00:52:56,900 --> 00:53:02,420
+يصنع أيش؟ RBC's كتيرة، تطلع RBCs زيادة على الـ
+
+670
+00:53:02,420 --> 00:53:05,420
+circulation، إذا أصبحت الحالة بتشبه الـ polycythemia
+
+671
+00:53:05,420 --> 00:53:10,500
+يعني زادت الـ RBCs، يعني بدها وقت أطول لأنها تترسب
+
+672
+00:53:10,500 --> 00:53:15,020
+يعني سرعتها هتكون بطيئة، هذا معناه أنه بدها وقت
+
+673
+00:53:15,020 --> 00:53:20,560
+أطول لتترسب، hypofibrogenemia يعني نقصان الفيبروجين
+
+674
+00:53:20,560 --> 00:53:26,470
+نقصان أحد الـ acute phase protein، تمام؟ نقصان الـ
+
+675
+00:53:26,470 --> 00:53:30,810
+fibrinogen اللي هو نقصان أحد الـ... الـ acute phase
+
+676
+00:53:30,810 --> 00:53:35,210
+protein بشكل طبيعي، يعني الجسم ما بيصنع fibrinogen
+
+677
+00:53:35,210 --> 00:53:39,230
+أقل من الطبيعي، فبالتالي برضه كمان هذا هيعمل لي
+
+678
+00:53:39,230 --> 00:53:44,310
+inhibition للـ aggregation و الـ raw formation of
+
+679
+00:53:44,310 --> 00:53:50,110
+RBCs، فبالتالي هيكون في عندي الـ ESR will decrease
+
+680
+00:53:50,750 --> 00:53:54,270
+كمان حالات الـ macrocytosis و الـ anisocytosis و
+
+681
+00:53:54,270 --> 00:53:58,730
+وجود... وجود spherocytes و sickle cell في الـ... مع
+
+682
+00:53:58,730 --> 00:54:03,070
+الخلايا أيضًا، يعني تباين في الحجم والشكل، هذا هيعمل
+
+683
+00:54:03,070 --> 00:54:05,330
+ليه؟ inhibition للـ roll off formation، إذا
+
+684
+00:54:05,330 --> 00:54:12,270
+decreased ESR، تمام؟ طيب خلينا نشوف كيف احنا بنشتغل
+
+685
+00:54:12,270 --> 00:54:16,970
+فحص الـ ESR، طبعًا في عندي نوعين أو طريقتين أنا بشتغل
+
+686
+00:54:16,970 --> 00:54:20,730
+فيهم، حسب نوع الـ tube اللي بشتغل فيها، نوعين من الـ
+
+687
+00:54:20,730 --> 00:54:25,750
+tubes وطريقتين، طبعًا ويستيرجرين method بستخدم فيها
+
+688
+00:54:25,750 --> 00:54:30,370
+أنابيب اسمها Westergren tube، طبعًا وفي عندي وينتروب
+
+689
+00:54:30,370 --> 00:54:34,950
+method بستخدم أنابيب اسمها Wintrobe tube، طبعًا، طيب
+
+690
+00:54:34,950 --> 00:54:41,630
+خلينا نشوف الـ... الـ... الـ Westergren method بيحكي لي أنا
+
+691
+00:54:41,630 --> 00:54:46,110
+ممكن أستخدم عينة، عينة طبعًا هو الـ blood طبيعي أكيد
+
+692
+00:54:46,110 --> 00:54:50,950
+يعني الدم كامل بدون فصل، أنا باخده، وطبعًا إذا بدي
+
+693
+00:54:50,950 --> 00:54:54,670
+آخذ مثلًا أربعة ملليلتر لازم أضيفه على one ml tube
+
+694
+00:54:54,670 --> 00:54:58,150
+فيها، أو على... على tube فيها one ml sodium
+
+695
+00:54:58,150 --> 00:55:03,460
+citrate، طبعًا؟ يعني النسبة أربعة لواحد، الـ dilution
+
+696
+00:55:03,460 --> 00:55:08,920
+هيكون كام؟ هيكون واحد لخمسة، مين اللي مخفف؟ الـ
+
+697
+00:55:08,920 --> 00:55:13,640
+citrate، والله اللي مخفف لأنه اللي هو الكمية الأقل
+
+698
+00:55:13,640 --> 00:55:18,840
+صح؟ واحد لخمسة لما أحكي dilution واحد لخمسة طبعًا؟
+
+699
+00:55:18,840 --> 00:55:23,800
+يعني أنَّ... إذا بدي أحكي خمسة لواحد بحكي عن الـ blood
+
+700
+00:55:24,830 --> 00:55:28,970
+طبعًا احنا أخذنا قوانين الـ dilution فبإمكانكم أنكم
+
+701
+00:55:28,970 --> 00:55:35,670
+تحسبوها وتعرفوا كم الـ dilution، طبعًا إذا أنا
+
+702
+00:55:35,670 --> 00:55:40,310
+استخدمت EDTA anticoagulated blood، يعني الـ blood
+
+703
+00:55:40,310 --> 00:55:43,490
+أنا ما حطيته في tube فيه، بعد ما جمعته ما حطيته في
+
+704
+00:55:43,490 --> 00:55:47,070
+tube فيها citrate، إنما حطيته في tube فيها EDTA
+
+705
+00:55:47,070 --> 00:55:53,470
+طبعًا، طبعًا فبيحكي لي أنَّ هاي الـ EDTA قبل ما أنا
+
+706
+00:55:53,470 --> 00:55:59,690
+أملأ... أملأ العينة بالـ Westergren tube لازم كل
+
+707
+00:55:59,690 --> 00:56:02,570
+اثنين ملليلتر من الـ blood الـ anticoagulated بالـ
+
+708
+00:56:02,570 --> 00:56:09,530
+EDTA أحط لهم نص ملليلتر من محلول ملح فيه تركيز
+
+709
+00:56:09,530 --> 00:56:12,450
+0.85% من sodium chloride اللي هو
+
+710
+00:56:12,450 --> 00:56:18,280
+الـ saline، تمام؟ أربعة ملليلتر بدي أحط لهم واحد ملليلتر
+
+711
+00:56:18,280 --> 00:56:22,980
+sodium chloride solution اللي هو إيش بنسميه؟ بنسميه
+
+712
+00:56:22,980 --> 00:56:26,600
+saline اللي هو المحلول الملحي اللي احنا ممكن نشتريه
+
+713
+00:56:26,600 --> 00:56:33,310
+من الـ... من الصيدلية، هو المحلول الملحي أيضًا اللي
+
+714
+00:56:33,310 --> 00:56:39,270
+احنا ممكن نحطه بالوريد للناس اللي بحتاجوا تخدير
+
+715
+00:56:39,270 --> 00:56:45,170
+وريدي، فبالتالي هذا هو المحلول الملحي مصمم بتركيز
+
+716
+00:56:45,170 --> 00:56:50,410
+0.85% Sodium Chloride لحتى يحافظ على شكل الخلية
+
+717
+00:56:50,410 --> 00:56:55,630
+على الـ osmotic pressure تبع الخلية، تمام؟ فيعني
+
+718
+00:56:55,630 --> 00:56:59,830
+بيستخدم لحتى أنا بضيفه على الـ EDTA tube، طب ليش
+
+719
+00:56:59,830 --> 00:57:02,070
+بضيفه على الـ EDTA؟ لأنَّ الـ EDTA مع الـ sodium
+
+720
+00:57:02,070 --> 00:57:05,410
+chloride بيعطيني نفس الـ osmotic pressure زي ما
+
+721
+00:57:05,410 --> 00:57:09,550
+أستخدم sodium citrate لحاله طبعًا، إذا يا إما أربعة
+
+722
+00:57:09,550 --> 00:57:14,190
+ملليلتر من الـ whole blood بضيفهم على tube فيها 1 ملليلتر من
+
+723
+00:57:14,190 --> 00:57:20,220
+sodium citrate أو أربعة ملليلتر من الـ EDTA بضيف لهم one
+
+724
+00:57:20,220 --> 00:57:26,100
+ml من الـ... الـ sodium chloride solution، تمام؟ و
+
+725
+00:57:26,100 --> 00:57:31,080
+بعد هيك بـ... يعني بعبي الـ... الـ... ويسترجرين tube
+
+726
+00:57:31,080 --> 00:57:41,200
+تمام؟ طيب ليش أنا ضفت على الـ EDTA... يعني ليش ضفت
+
+727
+00:57:41,200 --> 00:57:44,320
+على الـ EDTA saline؟ أول شيء... أول سبب علشان
+
+728
+00:57:44,320 --> 00:57:49,160
+أحافظ على الـ osmolality، تمام؟ وثاني شيء يا بنات
+
+729
+00:57:49,160 --> 00:57:53,900
+عشان... عامل الـ dilution، هلأ الـ sodium citrate...
+
+730
+00:57:53,900 --> 00:57:56,900
+الـ sodium citrate لما أنا أحط one ml volume هذا
+
+731
+00:57:56,900 --> 00:58:02,480
+تمام؟ هذا حجم الـ citrate سائل وأنا حطيت على الأربعة
+
+732
+00:58:02,480 --> 00:58:09,260
+ملليلتر من الـ blood one ملليلتر من هذا الـ citrate، طيب لكن الـ
+
+733
+00:58:09,260 --> 00:58:15,120
+EDTA بتكون يا بنات powder وجزء لا يكاد يذكر يكون
+
+734
+00:58:15,120 --> 00:58:19,060
+ممسوح في... في... في tube الـ EDTA، فلما أجي أنا
+
+735
+00:58:19,060 --> 00:58:24,610
+أضيف أربعة ملليلتر على tube الـ EDTA ما بتغيّر شيء أو
+
+736
+00:58:24,610 --> 00:58:27,790
+بيزيدوا بنسبة ضئيلة جدًا، يعني بظلوا أربعة زي ما
+
+737
+00:58:27,790 --> 00:58:30,990
+هما، هان أربعة أنا ضفت عليها one ml من الـ
+
+738
+00:58:30,990 --> 00:58:33,330
+saline، يعني صاروا الـ volume خمسة، الـ total volume
+
+739
+00:58:33,330 --> 00:58:37,190
+أما هنا الـ EDTA مع الـ blood لما أنا أضيف الـ blood
+
+740
+00:58:37,190 --> 00:58:41,030
+على الـ tube فيها EDTA، بيظلوا أربعة زي ما هو، لأنَّ
+
+741
+00:58:41,030 --> 00:58:46,050
+الـ EDTA بودرة وبتكون ذائبة يعني على جدار الـ
+
+742
+00:58:46,050 --> 00:58:51,330
+tube، كمية لا تكاد تذكر، فأنا لازم أضيف الـ one ml
+
+743
+00:58:51,330 --> 00:58:57,290
+solution من الـ saline solution علشان أحافظ على نفس
+
+744
+00:58:57,290 --> 00:59:01,150
+النسب، نفس الـ dilution، نفس الـ proportion، أنا أخذت
+
+745
+00:59:01,150 --> 00:59:06,130
+تمام؟ أتمنى أنَّها تكون واضحة، وبعد ما أنا أملأ
+
+746
+00:59:06,130 --> 00:59:09,750
+الـ Westergren pipe أو الـ Westergren tube بخليها
+
+747
+00:59:09,750 --> 00:59:16,410
+allow it to stand، بخليها أنَّها تكون... يعني بالـ vertical
+
+748
+00:59:16,410 --> 00:59:21,610
+position لمدة one hour وبعد هيك أنا بقيس المسافة
+
+749
+00:59:21,610 --> 00:59:24,430
+اللي تقطعها الـ RBC بالـ millimeter per one hour
+
+750
+00:59:24,430 --> 00:59:30,050
+المعدل الطبيعي للنساء من 0 إلى 15، عند الرجال من 0 إلى
+
+751
+00:59:30,050 --> 00:59:33,810
+10، أو عند الأطفال أيضًا من 0 إلى 10 millimeter per
+
+752
+00:59:33,810 --> 00:59:39,870
+hour، طبعًا عند النساء أكثر لأنَّ النساء يعني نسبة الـ
+
+753
+00:59:39,870 --> 00:59:43,870
+plasma protein عندهم مختلفة، تمام؟ بعدين النساء
+
+754
+00:59:43,870 --> 00:59:48,230
+بتكون يعني إما طالعة من... من period أو فائتة على
+
+755
+00:59:48,230 --> 00:59:51,730
+period أو يعني... يعني فالـ plasma protein والـ
+
+756
+00:59:51,730 --> 00:59:55,350
+tissue injury والحالات هذه يعني بتكون... بتأثر و
+
+757
+00:59:55,350 --> 00:59:59,270
+... فبالتالي هي نسبتها بتكون أعلى بشكل طبيعي، يعني
+
+758
+00:59:59,270 --> 01:00:00,090
+هذه بتكون أعلى
+
+759
+01:00:03,160 --> 01:00:10,460
+تمام؟ إذا هذا هو الـ normal ranges، طيب
+
+760
+01:00:10,460 --> 01:00:14,340
+إيش محتاجة؟ محتاجة للتجربة تبعي، عينة الـ blood
+
+761
+01:00:14,340 --> 01:00:17,720
+اللي أنا بدي أسحبها وأحطها في الـ... في الـ... إما
+
+762
+01:00:17,720 --> 01:00:21,940
+في سترات أو في EDTA tube، لو حطيتها في سترات خلاص
+
+763
+01:00:21,940 --> 01:00:26,390
+يعني هي 4 ml blood على 1 ml sodium citrate، وإذا
+
+764
+01:00:26,390 --> 01:00:29,110
+حطيتها في EDTA tube لازم أضيف one and a half ml من
+
+765
+01:00:29,110 --> 01:00:32,590
+الـ saline، عشان بالنهاية أنا بدي نفس النسبة ونفس
+
+766
+01:00:32,590 --> 01:00:35,810
+الـ dilution ونفس الـ proportion ونفس الـ osmolality
+
+767
+01:00:35,810 --> 01:00:39,550
+تمام؟ بعدين بملأ الـ Westergren tube زي ما
+
+768
+01:00:39,550 --> 01:00:42,830
+أنتم شايفين، الـ Westergren tube بهذا الشكل ولازم
+
+769
+01:00:42,830 --> 01:00:47,110
+يكون لها أبعاد، طبعًا احنا حكينا أنبوب خاص، يعني
+
+770
+01:00:47,110 --> 01:00:50,430
+ما قاش معنا specific tube، يعني أنها لازم تكون
+
+771
+01:00:50,430 --> 01:00:56,960
+أبعادها خاصة أو standard، الطول تبعها، الـ external
+
+772
+01:00:56,960 --> 01:01:00,940
+diameter، القطر الخارجي، القطر الداخلي، عليها تدريج
+
+773
+01:01:00,940 --> 01:01:07,690
+معين، هذا كله لازم يكون يعني ضمن معايير، وأيضًا بحتاج
+
+774
+01:01:07,690 --> 01:01:12,790
+الـ rack لهاي الـ... القنابيب، هذا اللي لونه أزرق هو
+
+775
+01:01:12,790 --> 01:01:17,430
+اللي بنسميه rack، تمام؟ وطبعًا كل الـ rackات لازم
+
+776
+01:01:17,430 --> 01:01:21,590
+تكون فيها حاجة زي الـ bulb بتأكد لي أنَّ هذا الـ rack
+
+777
+01:01:21,590 --> 01:01:26,850
+يعني جالس بشكل طبيعي أو بشكل يعني كويس على الـ
+
+778
+01:01:26,850 --> 01:01:31,730
+bench فهو عمودي، مئة بالمئة عمودي، تمام؟
+
+779
+01:01:34,970 --> 01:01:39,670
+يعني كيف هيكون فيه bulb، خليني بس أذكركم مثال
+
+780
+01:01:39,670 --> 01:01:44,270
+شوفتوا عامل البناء اللي بيكون بيبني جدران أو شيء
+
+781
+01:01:44,270 --> 01:01:47,970
+بيكون معه زي مصطرة حديدية بيسميها ميزان وهي
+
+782
+01:01:47,970 --> 01:01:52,110
+المصطرة الحديدية في نصها، في نصها، في أنبوبة زجاجية
+
+783
+01:01:52,110 --> 01:01:56,270
+فيها سائل أصفر، طبعًا هذا عامل البناء بيبني الجدار
+
+784
+01:01:56,270 --> 01:02:00,130
+بعد هيك، أو يعني لما بيجي بده يبني بيحط الـ... مصدر
+
+785
+01:02:00,130 --> 01:02:05,930
+الجدار تبعه وبيشوف مدى استواء أو اعتدال الجدار
+
+786
+01:02:06,660 --> 01:02:11,260
+لو كان في عندي خلل في مستوى الماء، يعني في إمالة
+
+787
+01:02:11,260 --> 01:02:15,660
+فيه، معناه هو الـ... الشيء مش عمودي أو مش مستوي، وهنا
+
+788
+01:02:15,660 --> 01:02:18,160
+نفس الفكرة، يعني لازم يكون في عندي bulb، لازم يكون
+
+789
+01:02:18,160 --> 01:02:22,020
+في عندي علامة من خلالها أعرف أنَّ هذا الـ... الـ rack
+
+790
+01:02:22,020 --> 01:02:27,010
+جالس بالشكل الـ vertical أو لا، وأيضًا كمان لازم يكون
+
+791
+01:02:27,010 --> 01:02:33,650
+في عندي timer لحتى يعني أضبطه على one hour ويـ
+
+792
+01:02:33,650 --> 01:02:37,890
+يحسب لي ساعة ويرن بعد نهاية الساعة عشان أجي أقرأ
+
+793
+01:02:37,890 --> 01:02:45,400
+النتائج، طيب إذا أنا إيش محتاجة؟ محتاجة إما عينة
+
+794
+01:02:45,400 --> 01:02:52,380
+blood أربعة ملليلتر في سترات one ml، أو أحط أربعة ملليلتر
+
+795
+01:02:52,380 --> 01:02:59,580
+من الـ EDTA وأحط عليهم one ml من الـ... الـ saline
+
+796
+01:02:59,580 --> 01:03:03,760
+أو كل اثنين ملليلتر أضيف لهم نص من الـ sodium chloride
+
+797
+01:03:03,760 --> 01:03:08,220
+solution اللي هو الـ saline، تمام؟ هي في عندكم point
+
+798
+01:03:08,220 --> 01:03:12,420
+0.85% weight per volume عندكم في محاضرة
+
+799
+01:03:12,420 --> 01:03:16,100
+للمناقشة إن شاء الله، هسألكم كيف حضرناها هذه عشان
+
+800
+01:03:16,100 --> 01:03:19,120
+تعرفوا لي الـ weight per volume، احنا كيف حضرنا محلول الـ
+
+801
+01:03:19,120 --> 01:03:19,500
+saline
+
+802
+01:03:22,320 --> 01:03:25,720
+طبعًا التجربة مش راح أشرحها لأنها شبه صارت واضحة، و
+
+803
+01:03:25,720 --> 01:03:30,940
+لأنَّ احنا راح نشتغل بالمعامل بطريقة مختلفة طبعًا، لا
+
+804
+01:03:30,940 --> 01:03:35,040
+هي Wintrobe ولا Westergren، طريقة مختلفة، فخلينا...
+
+805
+01:03:35,040 --> 01:03:37,520
+يعني أنتم هيك صرتوا أصلاً already فاهمين يعني إيش
+
+806
+01:03:37,520 --> 01:03:44,910
+التجربة أو كيف بتشتغل، ولكن يعني خلونا... نختصر
+
+807
+01:03:44,910 --> 01:03:52,430
+موضوع الـ procedure، Wintrobe and Landsberg method أو
+
+808
+01:03:52,430 --> 01:03:57,580
+اختصارًا بنحكي عنها Wintrobe method، تمام؟ الفكرة هنا
+
+809
+01:03:57,580 --> 01:04:02,000
+أنَّ أنا ممكن أستخدم EDTA Anti-coagulated blood
+
+810
+01:04:02,000 --> 01:04:05,940
+تمام؟ وعلى طول بس هيك أحط وأعبيه في tube اسمها
+
+811
+01:04:05,940 --> 01:04:11,620
+Wintrobe tube وأجلسها لمدة one hour بشكل على الوضع
+
+812
+01:04:11,620 --> 01:04:15,900
+الـ vertical وبعد هيك أقرأ النتائج، فعندي أنَّ النساء
+
+813
+01:04:15,900 --> 01:04:23,160
+المعدل الطبيعي من 0 إلى 20 والرجال
+
+814
+01:04:23,160 --> 01:04:31,090
+من 0 إلى 9، طبعًا فهذه هي الـ... الـ normal ranges للـ
+
+815
+01:04:31,090 --> 01:04:33,930
+Wintrobe tube، إيش محتاجة؟ محتاجة تيوبات Wintrobe
+
+816
+01:04:33,930 --> 01:04:37,890
+محتاجة الـ rack تبعهم، محتاجة... يعني ممكن أحتاج
+
+817
+01:04:37,890 --> 01:04:44,710
+capillary pipet أو يعني محتاجة a sticker يسكر طرف
+
+818
+01:04:44,710 --> 01:04:49,030
+الأنبوبة من جهة عشان أثبتها على الـ rack، بعد هيك و
+
+819
+01:04:49,030 --> 01:04:54,190
+محتاجة whole blood طبعًا one ml using EDTA
+
+820
+01:04:54,190 --> 01:05:00,730
+anticoagulant، نفس الفكرة عينة blood مجمّعة في EDTA
+
+821
+01:05:00,730 --> 01:05:05,790
+tube بعبي منها الـ capillary pipet أو يعني بعبي
+
+822
+01:05:05,790 --> 01:05:07,890
+منها باستخدام الـ capillary pipet اللي هي الـ Wintrobe
+
+823
+01:05:07,890 --> 01:05:13,650
+tube وبسكرها من طرفها وبعد هيك بحطها
+
+824
+01:05:13,650 --> 01:05:17,990
+على الـ rack بالـ vertical position لمدة ساعة وبقرأ
+
+825
+01:05:17,990 --> 01:05:22,360
+هنا، هَيْ هي الـ procedure تبعها، طبعًا خلينا بس نستعرض
+
+826
+01:05:22,360 --> 01:05:29,860
+كيف شكل الـ... الـ... الـ Wintrobe tube أو sorry، هاي
+
+827
+01:05:29,860 --> 01:05:34,840
+الـ Westergren، هاي Westergren tube مع الـ... الـ sodium
+
+828
+01:05:34,840 --> 01:05:37,780
+citrate tube اللي بيكون فيها one ml sodium citrate
+
+829
+01:05:37,780 --> 01:05:42,560
+وهذه هي الـ ESR tube مدرّجة، طبعًا أنا بفوتها وبكون
+
+830
+01:05:42,560 --> 01:05:47,620
+نهاية التدريج عند zero، طبعًا فالمنطقة الـ zero بتكون
+
+831
+01:05:47,620 --> 01:05:49,040
+في الأعلى طبعًا
+
+832
+01:05:55,410 --> 01:06:00,950
+هي... هذه... بحكي عنها Wintrobe tube، هي أسمك
+
+833
+01:06:00,950 --> 01:06:07,230
+شوية، تمام؟
+
+834
+01:06:07,230 --> 01:06:16,750
+تمام
+
+835
+01:06:20,360 --> 01:06:27,220
+طيب نيجي لمناقشة بعض النقاط المتعلقة بالفحص الـ ESR
+
+836
+01:06:27,220 --> 01:06:34,440
+أول شيء أثناء ترسيب خلايا الـ RBCs تمر عملية
+
+
+889
+01:10:38,750 --> 01:10:44,970
+فقدان للـ RBCs أكثر من الـ plasma فبالتالي هذا سيؤثر
+
+890
+01:10:44,970 --> 01:10:49,350
+على النتيجة ويجعلها لا تؤخذ بها، تمام؟ فلازم أعيد
+
+891
+01:10:49,350 --> 01:10:52,970
+الفحص. All the sedimentation racks should be
+
+892
+01:10:52,970 --> 01:10:56,170
+equipped with leveling screws and a spirit
+
+893
+01:10:56,170 --> 01:10:59,480
+level. لازم تكون هذه الـ... الـ racks اللي أنا
+
+894
+01:10:59,480 --> 01:11:04,700
+بستخدمها في عندها أمان أو ضابط أو شيء يعني يكون
+
+895
+01:11:04,700 --> 01:11:08,700
+دائماً يؤكد لي أن المستوى الذي أشتغل عليه مستوى
+
+896
+01:11:08,700 --> 01:11:15,440
+أفقي و الـ cube عمودي 100%، تمام؟ يصنع زاوية 90 درجة.
+
+897
+01:11:15,440 --> 01:11:22,240
+mechanical source of error، خلل أو مصدر خلل نتيجة
+
+898
+01:11:22,240 --> 01:11:29,090
+mechanical error. طيب إذا زاد تركيز الـ EDTA، طبعاً؟
+
+899
+01:11:29,090 --> 01:11:33,730
+إذا زاد تركيز الـ EDTA، طبعاً؟ فيعني... يعني...
+
+900
+01:11:33,730 --> 01:11:36,690
+ماذا يعني زاد تركيز الـ EDTA؟ أليس حكينا
+
+901
+01:11:36,690 --> 01:11:40,270
+أن القنابيب معلمة، يعني أنا لازم أعبيها حتى علامة
+
+902
+01:11:40,270 --> 01:11:45,360
+معينة، طبعاً لو عبيت القنابيب أقل من العلامة، يعني blood
+
+903
+01:11:45,360 --> 01:11:49,720
+نسبته أقل من اللازم بالنسبة للـ EDTA، يعني الـ
+
+904
+01:11:49,720 --> 01:11:53,880
+EDTA زادت، تمام؟ فإذا زادت الـ EDTA حكينا أنها
+
+905
+01:11:53,880 --> 01:11:57,620
+تُقلل الـ ESR، وهذه المعلومة مرت علينا قبل ذلك. طيب
+
+906
+01:11:57,620 --> 01:12:01,260
+لماذا؟ لأنها تعمل inhibition لـ roll-off
+
+907
+01:12:01,260 --> 01:12:06,900
+formation. الـ ESR يقل نتيجة inhibition للـ roll-off
+
+908
+01:12:06,900 --> 01:12:10,440
+formation لأن الـ EDTA إذا زادت ستعمل inhibition
+
+909
+01:12:10,440 --> 01:12:16,000
+للـ roll-off formation. طيب، أيضاً الـ anticoagulant
+
+910
+01:12:16,000 --> 01:12:20,680
+of sodium or potassium citrates، والهيبارين
+
+911
+01:12:20,680 --> 01:12:26,240
+يُسبب انكماشاً للـ RBCs، يعني
+
+912
+01:12:26,240 --> 01:12:31,600
+اضمحلالاً أو ضموراً للـ RBCs، أو يعني ستنكمش الـ RBCs
+
+913
+01:12:32,010 --> 01:12:36,390
+فبالتالي حجم الـ RBCs، الـ sediment تبعها سيقل.
+
+914
+01:12:36,390 --> 01:12:42,130
+البلازما ستزيد على حساب الـ RBCs، يعني ماذا؟ أنا أقيس
+
+915
+01:12:42,130 --> 01:12:45,310
+المسافة التي سقطتها، طيب هذه المسافة التي سقطتها
+
+916
+01:12:45,310 --> 01:12:48,090
+يعني سقطت مسافة وانكمشت نتيجة وجود الـ
+
+917
+01:12:48,090 --> 01:12:53,610
+anticoagulant، انكمشت. طبعاً هذا سيُزيد من قيمة ESR
+
+918
+01:12:53,610 --> 01:12:58,590
+falsely elevated، خلل. هذا ليس عالياً لأنه في خلل
+
+919
+01:12:58,590 --> 01:13:04,950
+عند المريض، لا هو عالي نتيجة خلل في سحب العينة أو في
+
+920
+01:13:04,950 --> 01:13:10,590
+اختيار النوع المناسب للـ anticoagulant. إذا if the
+
+921
+01:13:10,590 --> 01:13:14,590
+ESR stands for more than 60 minutes, the result will
+
+922
+01:13:14,590 --> 01:13:17,850
+be falsely elevated. و أيضاً لو نسيت الـ tube
+
+923
+01:13:17,850 --> 01:13:23,350
+أكثر من ساعة، ممكن أيضاً نتيجة ESR تكون مرتفعة
+
+924
+01:13:23,350 --> 01:13:28,390
+تمام؟ وهذا طبعاً إذا كان أيضاً الـ... الـ... الوقت
+
+925
+01:13:28,390 --> 01:13:33,210
+أقل من ساعة، هذا أيضاً ممكن أن يُعطيني low value، فـ
+
+926
+01:13:33,210 --> 01:13:38,670
+...و invalid، يعني لا تؤخذ بها طبعاً. Mark the
+
+927
+01:13:38,670 --> 01:13:41,890
+increase or decrease in room temperature leads to
+
+928
+01:13:41,890 --> 01:13:45,170
+increase or decrease in ESR result.  حكينا زيادة
+
+929
+01:13:45,170 --> 01:13:49,270
+الـ room temperature، كلما زادت درجة الحرارة ستزيد
+
+930
+01:13:49,270 --> 01:13:54,360
+قيمة الـ ESR. Tilting of the ESR tube increases the
+
+931
+01:13:54,360 --> 01:13:59,020
+ESR. إمالة الأنبوب، يعني أنها ليست في الـ
+
+932
+01:13:59,020 --> 01:14:02,980
+vertical position، تكون مائلة، ستزيد الـ ESR، وحكينا
+
+933
+01:14:02,980 --> 01:14:07,920
+لماذا؟ لأن يصير عندي مساحة سطح بسبب الميلان، هذا
+
+934
+01:14:07,920 --> 01:14:11,920
+يصير عندي مساحة سطح للـ roll-off formation أكبر
+
+935
+01:14:11,920 --> 01:14:17,820
+طبعاً؟ طيب. Bubbles in blood cause invalid results.
+
+936
+01:14:17,820 --> 01:14:22,840
+فقاعات من الهواء في الأنابيب
+
+937
+01:14:23,760 --> 01:14:27,340
+أيضاً ستؤثر على النتيجة، ستعطيني invalid
+
+938
+01:14:27,340 --> 01:14:32,120
+result، و...لأن أيضاً أنا أقيس مسافة، فلو
+
+939
+01:14:32,120 --> 01:14:35,680
+هذه المسافة مشغولة، يعني فيها فقاعات، يعني إذا
+
+940
+01:14:35,680 --> 01:14:40,320
+القراءة لن تكون دقيقة. Fibrin clots present
+
+941
+01:14:40,320 --> 01:14:43,500
+in the blood invalidate results. أيضاً لو يصير
+
+942
+01:14:43,500 --> 01:14:47,390
+عندي تخثر في عينة الدم، سيؤثر على
+
+943
+01:14:47,390 --> 01:14:51,230
+النتيجة، سيجعلها لا تؤخذ بها، وهذا الـ fibrin
+
+944
+01:14:51,230 --> 01:14:54,290
+clot،  ممكن أن تظهر نتيجة عدم قيامي بخلط
+
+945
+01:14:54,290 --> 01:14:58,260
+العينة مع الـ anticoagulant بشكل كافٍ، فيصير في
+
+946
+01:14:58,260 --> 01:15:02,640
+تخثر، يعني تجمع للـ RBCs، بدون أن يصير في
+
+947
+01:15:02,640 --> 01:15:07,020
+تجانس، يعني ستترسب بسرعة، وهذا يعني نتيجة خاطئة.
+
+948
+01:15:07,020 --> 01:15:12,200
+The ESR should be set within two hours of
+
+949
+01:15:12,200 --> 01:15:16,520
+blood collection. أنا لازم أشتغل الـ ESR خلال ساعتين
+
+950
+01:15:16,520 --> 01:15:21,180
+من جمع العينة، إذا أنا أستخدم، يعني إذا استخدمت الـ
+
+951
+01:15:21,180 --> 01:15:26,120
+EDTA anticoagulant، ممكن أن يعطيني ست ساعات، يعني وقت
+
+952
+01:15:26,120 --> 01:15:32,380
+يعني ما نحكي عنه، يعني الوقت الفاصل ما بين جمع
+
+953
+01:15:32,380 --> 01:15:37,440
+العينة، ويعني أني أجي أشتغلها، ممكن معي حتى ست
+
+954
+01:15:37,440 --> 01:15:40,300
+ساعات إذا كانت العينة مبردة باستخدام الـ
+
+955
+01:15:40,300 --> 01:15:45,480
+EDTA، تمام؟ باستخدام set rate؟ لا، ساعتين، تمام.
+
+956
+01:15:45,480 --> 01:15:47,660
+الآن الـ Westergren ESR
+
+957
+01:15:50,850 --> 01:15:55,510
+طبعاً أنبوبة Westergren more sensitive، لو الـ ESR له
+
+958
+01:15:55,510 --> 01:16:03,970
+طبعاً، يعني لو الـ ESR قليل، أي... يعني أي تغير في
+
+959
+01:16:03,970 --> 01:16:09,190
+قيمته، هي... هي... يعني ستتمكن من أن
+
+960
+01:16:09,190 --> 01:16:14,010
+تكشف عنه، طبعاً إذا sensitive، أي تغير في الـ ESR
+
+961
+01:16:14,010 --> 01:16:18,070
+الذي قيمته قليلة، Westergren method أفضل له
+
+962
+01:16:19,650 --> 01:16:25,710
+ممكن أن تُجري detection، أما Westergren method، هي
+
+963
+01:16:25,710 --> 01:16:30,630
+more accurate لو كان الـ ESR عالياً، تمام؟ و
+
+964
+01:16:30,630 --> 01:16:33,510
+Westergren method has been chosen as the standard
+
+965
+01:16:33,510 --> 01:16:36,110
+يعني هي الـ standard method والمتّبعة أكثر.
+
+966
+01:16:36,110 --> 01:16:40,330
+Westergren method. When reading the ESR result on
+
+967
+01:16:40,330 --> 01:16:43,930
+blood with an extremely high WBCs or platelet
+
+968
+01:16:43,930 --> 01:16:48,050
+count, the buffy coat should be excluded. نفس فكرة
+
+969
+01:16:48,050 --> 01:16:51,450
+الهيماتوكريت، لما كنت أقيس الهيماتوكريت كنت أقيس
+
+970
+01:16:51,450 --> 01:16:56,510
+نسبة الـ RBCs فقط بدون الـ buffy coat. طبعاً، وأيضاً لما
+
+971
+01:16:56,510 --> 01:17:00,290
+أريد أن أقيس الـ ESR، الترسيب، سأرى المسافة التي قطعتها
+
+972
+01:17:00,290 --> 01:17:03,550
+يعني مسافة البلازما أو الحجم... المسافة التي
+
+973
+01:17:03,550 --> 01:17:11,010
+احتلتها البلازما طبعاً، و... يعني هذا بدون أن
+
+974
+01:17:11,010 --> 01:17:13,790
+أقرأ الـ buffy coat، يعني should be excluded.
+
+975
+01:17:13,790 --> 01:17:17,290
+يعني الـ buffy coat يكون خارج القراءة.
+
+976
+01:17:17,290 --> 01:17:21,450
+If there is poor separation of the RBCs and the
+
+977
+01:17:21,450 --> 01:17:27,660
+plasma layer, this may be due to an increased number of
+
+978
+01:17:27,660 --> 01:17:31,300
+reticulocytes، و أسميها stratified sedimentation. الآن
+
+979
+01:17:31,300 --> 01:17:37,200
+ماذا تعني هذه الجملة؟ الآن في نوع من الخلايا أو يعني
+
+980
+01:17:37,200 --> 01:17:42,720
+مرحلة من مراحل نضج الـ RBCs أسميها reticulocyte.
+
+981
+01:17:42,720 --> 01:17:46,900
+مرحلة الـ reticulocyte هي المرحلة التي تسبق خروجها
+
+982
+01:17:46,900 --> 01:17:51,990
+مباشرة إلى الـ circulation. طبعاً هي نفس شكل
+
+983
+01:17:51,990 --> 01:17:55,490
+الخلية الـ RBCs، نعرف أنها أثناء نضجها تمر
+
+984
+01:17:55,490 --> 01:17:59,850
+بمراحل في الـ bone marrow، آخر مرحلة في الـ bone
+
+985
+01:17:59,850 --> 01:18:02,550
+marrow تكون الـ reticulocyte، وبعدين تخرج إلى الـ
+
+986
+01:18:02,550 --> 01:18:07,050
+circulation. طبعاً الـ reticulocyte تشبه الخلية التي
+
+987
+01:18:07,050 --> 01:18:09,810
+موجودة في الـ circulation، الخلية التي نعرفها
+
+988
+01:18:09,810 --> 01:18:17,040
+التي هي شكلها... تمام؟ ولكن يكون فيها... يعني كشبكة
+
+989
+01:18:17,040 --> 01:18:21,620
+RNA، لم تتخلص من RNA تبعها، تخرج... يعني
+
+990
+01:18:21,620 --> 01:18:24,700
+نسبة reticulocytes بشكل طبيعي في جسم الإنسان
+
+991
+01:18:24,700 --> 01:18:29,280
+1%، 1 إلى 2%... هكذا يعني.
+
+992
+01:18:29,280 --> 01:18:33,980
+تخرج هذه reticulocytes إلى الدم، تأخذ لها يوم و
+
+993
+01:18:33,980 --> 01:18:38,440
+تنضج، يعني... خلاص يختفي RNA، وتصبح الـ
+
+994
+01:18:38,440 --> 01:18:44,330
+RBCs طبيعية. طيب، increase number of reticulocytes، متى
+
+995
+01:18:44,330 --> 01:18:49,710
+يزيد هذا العدد من reticulocytes؟ متى تزيد نسبة
+
+996
+01:18:49,710 --> 01:18:53,610
+الـ reticulocytes في الـ circulation، وتزيد نسبتها، و
+
+997
+01:18:53,610 --> 01:18:57,930
+يزيد إنتاجها، أو خروجها من الـ... يعني من الـ bone
+
+998
+01:18:57,930 --> 01:19:05,250
+marrow؟ نتيجة blood loss، يعني فقدان دم، أنيميا، تكسر
+
+999
+01:19:05,250 --> 01:19:10,750
+خلايا الـ RBCs، أي مرض ممكن أن... أن... ينتج عنه
+
+1000
+01:19:10,750 --> 01:19:15,170
+فقدان RBCs،  ممكن الـ bone marrow يُصبح تحت ضغط
+
+1001
+01:19:15,170 --> 01:19:19,090
+أن والله نحتاج إلى إنتاج أكثر، يعني إنتاج أكثر RBCs، نحن
+
+1002
+01:19:19,090 --> 01:19:22,990
+نفقد RBCs، تمام؟ فبالتالي ممكن يخرج الـ
+
+1003
+01:19:22,990 --> 01:19:25,410
+reticulocytes، فيها غير ناضجة، ولكن يلا، هذا يُساعدكم
+
+1004
+01:19:25,410 --> 01:19:29,920
+فيها شيء، يعني تحتاج ليوم لتُصبح ناضجة، طبعاً؟ طيب، هذه الـ
+
+1005
+01:19:29,920 --> 01:19:34,420
+reticulocytes مع خلايا الدم الطبيعية الموجودة في
+
+1006
+01:19:34,420 --> 01:19:39,100
+الدم، مختلفة، اختلاف بينهما في الكثافة، في
+
+1007
+01:19:39,100 --> 01:19:44,840
+الحجم، في... في الوزن، يعني هي نفس الشكل تقريباً
+
+1008
+01:19:44,840 --> 01:19:48,980
+لكن حجمها أكبر قليلاً، كثافتها مختلفة، فوزنها
+
+1009
+01:19:48,980 --> 01:19:54,260
+مختلف. طبعاً، فلما تأتي لتترسب، لا تترسب كلها بطبقة واحدة
+
+1010
+01:19:54,260 --> 01:19:58,900
+الخلايا، يصير عندي ترسيب مخطط، stratified
+
+1011
+01:19:58,900 --> 01:20:03,280
+sedimentation، يعني طبقة خلايا، بلازما، طبقة خلايا
+
+1012
+01:20:03,280 --> 01:20:10,100
+بلازما، وهكذا، طبعاً فيصير عندي... يعني مثل البوب،
+
+1013
+01:20:10,100 --> 01:20:15,500
+يصير مخطط، RBCs أحمر، أصفر، أحمر، أصفر، مثل هذا طبعاً
+
+1014
+01:20:15,500 --> 01:20:19,650
+RBCs، بلازما، RBCs، بلازما، وهكذا. نتيجة وجود
+
+1015
+01:20:19,650 --> 01:20:24,710
+reticulocytosis، أو زيادة في نسبة الـ reticulocytes.
+
+1016
+01:20:24,710 --> 01:20:28,370
+The internal bore and the length of a graduated
+
+1017
+01:20:28,370 --> 01:20:30,670
+scale on the Westergren pipe are critical
+
+1018
+01:20:30,670 --> 01:20:34,990
+measurements. حكينا قطرها وطولها للأنبوب لازم يكون
+
+1019
+01:20:34,990 --> 01:20:39,510
+standard. أنا أستخدم قنابيب خاصة، لأن القنابيب إذا
+
+1020
+01:20:39,510 --> 01:20:43,940
+كانت مثلاً، إذا زاد ارتفاعها، يعني لا توجد مساحة سطح كافية
+
+1021
+01:20:43,940 --> 01:20:48,700
+لـ... لـ... للـ ESR، ممكن أن يقل لأن لا توجد مساحة سطح
+
+1022
+01:20:48,700 --> 01:20:51,860
+كافية للـ roll-off formation، يقل الـ ESR. وأنابيب
+
+1023
+01:20:51,860 --> 01:20:55,640
+سميكة، يعني مساحة سطح أكبر للـ roll-off formation
+
+1024
+01:20:55,640 --> 01:21:00,680
+سيزيد الـ ESR، تمام؟ فبالتالي أنه يجب أن تكون الأنابيب
+
+1025
+01:21:00,680 --> 01:21:03,580
+standard لما أجي أشتغل فيها، لأجل أن أحصل على نتائج
+
+1026
+01:21:03,580 --> 01:21:04,640
+accurate and precise.
+
+1027
+01:21:09,520 --> 01:21:16,080
+disposable ESR. مرت معنا هذه الجملة أو هذا المصطلح
+
+1028
+01:21:16,080 --> 01:21:21,580
+disposable، طبعاً ومعناها استخدام لمرة واحدة فقط، و
+
+1029
+01:21:21,580 --> 01:21:25,160
+بعدين هيطلع disposable يعني one use، لما أحكي لكم
+
+1030
+01:21:25,160 --> 01:21:29,560
+هذه الأداة disposable، ماذا يعني؟ يعني استخدميها، و
+
+1031
+01:21:29,560 --> 01:21:34,020
+بعدين تخلصي منها، أرميها، طبعاً. لا نُعيد استخدامها.
+
+1032
+01:21:34,020 --> 01:21:37,990
+نستخدمها مرة أخرى. الآن، في Westergren، وفي Wintrobe،
+
+1033
+01:21:37,990 --> 01:21:42,230
+تيوب زجاجية، نغسلها، ونُعقمها بعد كل استخدام، و
+
+1034
+01:21:42,230 --> 01:21:45,690
+نُعيد استخدامها مرة أخرى. لكن في أنابيب بلاستيكية
+
+1035
+01:21:45,690 --> 01:21:49,330
+مثل الموجودة في الخلفية، هذه، استخدام لمرة واحدة
+
+1036
+01:21:49,330 --> 01:21:53,490
+فقط، ويتم التخلص منها. هذه تُسمى disposable ESR.
+
+1037
+01:21:53,490 --> 01:21:59,610
+فعندي commercial kits،  كتات تجارية أصبحت متاحة
+
+1038
+01:21:59,610 --> 01:22:04,550
+أصبحت now available للـ disposable ESR. طبعاً الـ
+
+1039
+01:22:04,550 --> 01:22:07,830
+civil kit include a safety cap for the tubes that allow
+
+1040
+01:22:07,830 --> 01:22:10,970
+the blood to precisely fill to the zero. بيكون طبعاً
+
+1041
+01:22:10,970 --> 01:22:16,490
+فيه مثل صمام موجود في... في هذه الأنبوبة، وطبعاً
+
+1042
+01:22:16,490 --> 01:22:20,250
+أثناء دخول أنا... يعني أنا هذه الأنبوبة لها صمام، و
+
+1043
+01:22:20,250 --> 01:22:23,070
+أدخلها داخل عينة الدم، أنبوبة الدم التي أنا
+
+1044
+01:22:23,070 --> 01:22:28,040
+سحبتها، تمام؟ وهذا الصمام يسمح بأن...
+
+1045
+01:22:28,040 --> 01:22:32,580
+الأنبوبة تمتلئ بالـ blood حتى ماذا؟ حتى
+
+1046
+01:22:32,580 --> 01:22:36,480
+علامة الـ zero، هذه.  لو جئتم هنا، نكبر
+
+1047
+01:22:36,480 --> 01:22:42,820
+الصورة، ونرى في الخلفية هذه الأنبوبة، zero، خمسة،
+
+1048
+01:22:42,820 --> 01:22:47,080
+عشرة، تمام؟  حكينا أن الأنابيب يجب أن تُملأ حتى
+
+1049
+01:22:47,080 --> 01:22:51,940
+الـ zero blood،  مثل هذا، حتى الـ zero، أملأها، لا
+
+1050
+01:22:51,940 --> 01:22:56,900
+تكون... لو وصلت إلى الواحد، إلى الاثنين، إلى الثلاثة... الـ...
+
+1051
+01:22:56,900 --> 01:23:00,740
+هنا، ممكن أن أحلّ المشكلة by subtraction،
+
+1052
+01:23:00,740 --> 01:23:03,900
+يعني أطرح في النهاية القيمة التي تظهر معي من
+
+1053
+01:23:03,900 --> 01:23:10,310
+كمية الفاقد أو النقص في الـ volume، طبعاً لو كانت يعني
+
+1054
+01:23:10,310 --> 01:23:14,070
+أقل من الخمسة، يعني يوجد عندي فاقد أكثر من خمسة
+
+1055
+01:23:14,070 --> 01:23:19,770
+تمام؟ فبالتالي هذه invalid result.  طيب نحن نحكي
+
+1056
+01:23:19,770 --> 01:23:24,440
+أن في الـ disposable ESR tube فيها صمام،
+
+1057
+01:23:24,440 --> 01:23:29,160
+يسمح بمرور الأنبوبة داخل عينة الدم ويرتفع فيها الدم إلى
+
+1058
+01:23:29,160 --> 01:23:33,520
+نسبة الـ... الـ zero level، تمام، بشكل دقيق، وهذا
+
+1059
+01:23:33,520 --> 01:23:37,360
+يجعل by  a closed system، نظام
+
+1060
+01:23:37,360 --> 01:23:4
\ No newline at end of file
diff --git a/PL9fwy3NUQKwY9jl8xw-bWkVZkWcGGyspd/Bu-y4fC_shI_postprocess.srt b/PL9fwy3NUQKwY9jl8xw-bWkVZkWcGGyspd/Bu-y4fC_shI_postprocess.srt
new file mode 100644
index 0000000000000000000000000000000000000000..0cc61a5619a8d04a93c4d7971ebb487d51d22172
--- /dev/null
+++ b/PL9fwy3NUQKwY9jl8xw-bWkVZkWcGGyspd/Bu-y4fC_shI_postprocess.srt
@@ -0,0 +1,4416 @@
+1
+00:00:02,830 --> 00:00:06,730
+السلام عليكم ورحمة الله وبركاته طالبات العزيزات
+
+2
+00:00:06,730 --> 00:00:09,970
+اليوم ان شاء الله راح نشرح محاضرة erythrocyte
+
+3
+00:00:09,970 --> 00:00:14,570
+sedimentation rate erythrocyte معناها red blood
+
+4
+00:00:14,570 --> 00:00:19,980
+cell خلايا الدم الحمراء sedimentationمعناها ترسيب
+
+5
+00:00:19,980 --> 00:00:25,240
+rate معدل، إذا ما عملنا اليوم بعنوان erythrocyte
+
+6
+00:00:25,240 --> 00:00:29,500
+sedimentation rate أي معدل ترسيب خلايا الدم
+
+7
+00:00:29,500 --> 00:00:35,900
+الحمراء، فيما يعرف بـ ESR تمام؟ اختصار لإله ESRقبل
+
+8
+00:00:35,900 --> 00:00:39,600
+ما نعرض ال slides خلونا بس مع بعض نشوف هاي الصورة
+
+9
+00:00:39,600 --> 00:00:43,340
+و هاي الصورة طبعا مش غريبة علينا يا بنات احنا
+
+10
+00:00:43,340 --> 00:00:47,560
+عرضناها في المعامل السابقة و تحدثنا عن ال blood
+
+11
+00:00:47,560 --> 00:00:51,140
+component حكينا لو انا عندي عينة blood في tube
+
+12
+00:00:51,140 --> 00:00:55,620
+وفصلتها عملتلها separation on centrifuge باستخدام
+
+13
+00:00:55,620 --> 00:01:00,900
+جهاز الطرد المركزي فرح تفصل العينة الى 3 طبقات
+
+14
+00:01:01,150 --> 00:01:04,170
+الطبقة اللى بتكون موجودة في البطم هي طبقة ال red
+
+15
+00:01:04,170 --> 00:01:08,790
+blood cell على أساس إنها هي ال red blood cell أو
+
+16
+00:01:08,790 --> 00:01:15,710
+ال RBCs على أساس إن هاي الطبقة بتكون الأسقل تمام و
+
+17
+00:01:15,710 --> 00:01:22,030
+تحتل ما بين 44 إلى 45% من ال total volume of whole
+
+18
+00:01:22,030 --> 00:01:26,690
+bloodو يليها طبقة ال buffy coat اللى هى الطبقة
+
+19
+00:01:26,690 --> 00:01:31,570
+الكريمية اللى بتكون مكونة من او يعني تحتوي على ال
+
+20
+00:01:31,570 --> 00:01:35,650
+white blood cell and the platelet طبعا خلايا الدم
+
+21
+00:01:35,650 --> 00:01:41,370
+البيضاء و الصفائح الدموية هذه الطبقة لا تتجاوز ال
+
+22
+00:01:41,370 --> 00:01:46,450
+1% طبعا او احيانا نحكي تجاوزا نحكي انها 1% من ال
+
+23
+00:01:46,450 --> 00:01:51,590
+total blood volume او ال whole blood volumeطيب و
+
+24
+00:01:51,590 --> 00:01:57,850
+يلي هاي طبقة طبقة ال plasma اللى بتحتال 55% من ال
+
+25
+00:01:57,850 --> 00:02:01,730
+holy blood volume و هى طبعا بتكون موجودة at the
+
+26
+00:02:01,730 --> 00:02:06,600
+top على أساس أنها هى أخف طبقة طبعا؟طيب بس اليوم
+
+27
+00:02:06,600 --> 00:02:09,920
+انا مش عم بذكر هاي ال .. ال .. او مش عم بعرض هاي
+
+28
+00:02:09,920 --> 00:02:17,680
+الصورة لحتى فقط نذكر مكونات ال .. ال .. الدم لأ
+
+29
+00:02:17,680 --> 00:02:23,280
+انا اليوم بدي .. بدي احكي انه عينت الدم طالما انه
+
+30
+00:02:23,280 --> 00:02:27,960
+انا ساحبتها و عملتلها mixing يعني وضعتها في tube و
+
+31
+00:02:27,960 --> 00:02:32,240
+عملتلها mixing مع anticoagulantAnticoagulant يعني
+
+32
+00:02:32,240 --> 00:02:37,580
+مانع للتخثر سواء كان هذا مانع التخثر edita أو
+
+33
+00:02:37,580 --> 00:02:42,420
+citrate أو heparin أو أي نوع من أنواع موانع التخثر
+
+34
+00:02:42,420 --> 00:02:46,840
+اللي تم ذكرها أو يعني موجودة في محاضرة ل blood
+
+35
+00:02:46,840 --> 00:02:53,290
+componentتمام؟ إذا تم سحب عينة وعملنا لها mixing
+
+36
+00:02:53,290 --> 00:02:57,690
+مع anticoagulant يعني منعنا تخثر هذه العينة، منعنا
+
+37
+00:02:57,690 --> 00:03:02,130
+تجلطها، إذا يبقى الدم في الصورة السائلة لإله وإذا
+
+38
+00:03:02,130 --> 00:03:06,530
+بقيت دم في الصورة السائلة لإله، فهو يا بنات، يعني
+
+39
+00:03:06,530 --> 00:03:10,230
+tend to يميل إلى أنه يفصل لو أنا حطيته على الbench
+
+40
+00:03:10,230 --> 00:03:14,490
+لمدة يعني عشر دقائق، ربع ساعة، بيكون بلش يفصلطبعا
+
+41
+00:03:14,490 --> 00:03:20,530
+أنا بستخدم ال centrifuge لحتى أسرع العملية هذه
+
+42
+00:03:20,530 --> 00:03:24,810
+دقيقة تان تلاتة خمس دقائق أنا بكون انتهت من عملية
+
+43
+00:03:24,810 --> 00:03:30,930
+الفصل طبعا لكن ال blood في الصورة السائلة لإله هو
+
+44
+00:03:30,930 --> 00:03:35,230
+يميل إلى أنه يفصل بحيث أن ال RBC بفعل الجاذبية
+
+45
+00:03:35,230 --> 00:03:39,770
+بتترسب لتحت و بتفصل ل plasma at the top يعني بشكل
+
+46
+00:03:39,770 --> 00:03:44,060
+طبيعي بس بدها تاخد وقت هذه العملية طبعاو هذه هي
+
+47
+00:03:44,060 --> 00:03:50,760
+فكرة فحصنا لليوم يعني فكرة فحصنا لليوم بتحكي انه
+
+48
+00:03:50,760 --> 00:03:56,500
+خلينا بصناعد الصورة هاي ال ESR الاريثروسايت
+
+49
+00:03:56,500 --> 00:04:00,880
+سيديمنتاشن ريت فكرة الفحص طبعه انه انا باستخدام
+
+50
+00:04:00,880 --> 00:04:05,420
+انابيب خاصة يا بنات و ركزولي على كلمة انابيب خاصة
+
+51
+00:04:05,420 --> 00:04:08,700
+راح نذكر ايش أنواع الانابيب اللي انا بستخدمها
+
+52
+00:04:08,700 --> 00:04:14,800
+لاحقا ولكنباستخدام أنابيب خاصة أنا بجمع عينة عينة
+
+53
+00:04:14,800 --> 00:04:19,900
+holy blood دم و بعملها mixing مع anticoagulant
+
+54
+00:04:19,900 --> 00:04:23,440
+يفضل إن أنا أستخدم ال citrate ال sodium citrate
+
+55
+00:04:23,440 --> 00:04:28,700
+anticoagulant و بعد هيك أنا بحطها على ال bench
+
+56
+00:04:28,700 --> 00:04:33,400
+بشكل عمودي تمام؟ بشكل عمودي يعني إنها تصنع مع ال
+
+57
+00:04:33,400 --> 00:04:38,240
+bench زاوية 90 درجة تمام؟ لازم تكون vertical أو
+
+58
+00:04:38,240 --> 00:04:41,410
+perpendicular to the benchتمام؟ بحطها في راك و
+
+59
+00:04:41,410 --> 00:04:45,590
+الراك بأضمن أنها هي .. هاي ال .. ال .. النبوبة بت
+
+60
+00:04:45,590 --> 00:04:51,390
+.. بشكل .. باتجاه عمودي و تصنع زاوية 90 درجة مع
+
+61
+00:04:51,390 --> 00:04:56,270
+المستوى اللي .. اللي هي موجودة عليه و بستن عليها
+
+62
+00:04:56,270 --> 00:05:00,600
+ساعةلأن حكينا اللي هو ال blood في صورته السائلة
+
+63
+00:05:00,600 --> 00:05:05,440
+يميل إلى إنه يفصل فال RBCs راح تتجه إلى ال bottom
+
+64
+00:05:05,440 --> 00:05:13,160
+و ال plasma راح تفصل بالنسبة للقراقة أو النتيجة
+
+65
+00:05:13,160 --> 00:05:18,520
+اللي أنا بدي أقراها أنا طبعا بقيس أو يعنيبدي قراءة
+
+66
+00:05:18,520 --> 00:05:23,340
+rate معدل يعني سرعة تمام؟ و احنا يمكن أخدنا
+
+67
+00:05:23,340 --> 00:05:28,220
+بالفيزيا ان السرعة هي عبارة عن المسافة على الزمن
+
+68
+00:05:28,220 --> 00:05:34,500
+تمام؟ طيب، اذا طبعا انا بما اني بدي معدل ترسيب ال
+
+69
+00:05:34,500 --> 00:05:38,820
+RBCs اذا انا بدي المسافة اللي تقطعها ال RBCs لما
+
+70
+00:05:38,820 --> 00:05:44,180
+بتترسب على الزمن تمام؟ اذا انا بقيس ال plasma
+
+71
+00:05:45,090 --> 00:05:50,210
+volume أو مسافة ال plasma هذه المسافة أنا بقيسها و
+
+72
+00:05:50,210 --> 00:05:54,870
+بقسمها على الزمن هذه المسافة يا بنات كانت مشغولة
+
+73
+00:05:54,870 --> 00:06:01,410
+بال RBC لما كان ال blood well mixed يعني ماعمله
+
+74
+00:06:01,410 --> 00:06:07,390
+mixing منيحتمام؟ بمجرد انه ترى السبق يعني ال RBCs
+
+75
+00:06:07,390 --> 00:06:10,110
+قطعت هذه المسافة كلها لحتى صارت في ال sediment
+
+76
+00:06:10,110 --> 00:06:15,450
+تمام؟ اذا انا بدي اقيس هذه المسافة على الزمن على
+
+77
+00:06:15,450 --> 00:06:18,110
+العكس من ال hematocrite ال hematocrite انا ايش كنت
+
+78
+00:06:18,110 --> 00:06:22,770
+اعمل؟ كنت اقيس نسبة ال .. ال RBCs يعني تمام؟
+
+79
+00:06:22,770 --> 00:06:26,090
+بالنسبة لل whole blood volume اعتبرها انها هي 100%
+
+80
+00:06:26,090 --> 00:06:30,890
+وشوف كام نسبة ال RBCs منها في الميةولكن في ال ESR
+
+81
+00:06:30,890 --> 00:06:35,510
+لأ أنا بقيس المسافة ل plasma على ال .. ال .. الزمن
+
+82
+00:06:35,510 --> 00:06:40,090
+اللي هي one hour تمام؟ اذا هذه هي فكرة فحصنا لليوم
+
+83
+00:06:40,090 --> 00:06:43,890
+علشان لما نشرح كمان شوية او نبلش بالشرح بعرض ال
+
+84
+00:06:43,890 --> 00:06:50,430
+slides انكون على الأقل متخيلين ايش يعني فحص ال ESR
+
+85
+00:06:51,000 --> 00:06:54,160
+Erythrocyte Sedimentation Rate حكينا إنه أنا
+
+86
+00:06:54,160 --> 00:06:59,500
+باستخدام أنابيب خاصة بملأها بيه ال blood اللي well
+
+87
+00:06:59,500 --> 00:07:04,280
+mixed تمام صارله mixing منيح مع anticoagulant و
+
+88
+00:07:04,280 --> 00:07:10,380
+طبعا بملأ هاي الأنابيب و بجلسها بشكل عمودي تمام
+
+89
+00:07:10,380 --> 00:07:14,820
+على راجوطبعا بيكون في خلفية ال rack فيه تدريج و
+
+90
+00:07:14,820 --> 00:07:18,800
+بعد ساعة مرور ساعة من الزمن أنا بقيس المسافة اللي
+
+91
+00:07:18,800 --> 00:07:22,600
+قطعتها ال RBC يعني مسافة البلازما هاي فكرة الفحص
+
+92
+00:07:22,600 --> 00:07:27,020
+طبعا الاريثروسيت سيديمنتاشن ريت الاريثروسيت
+
+93
+00:07:27,020 --> 00:07:32,740
+سيديمنتاشن ريت ESR is a non specific measurement
+
+94
+00:07:32,740 --> 00:07:37,640
+used to detect and monitoran inflammatory response
+
+95
+00:07:37,640 --> 00:07:41,740
+to tissue injury and acute phase in which there is
+
+96
+00:07:41,740 --> 00:07:44,400
+a change in plasma concentration of several
+
+97
+00:07:44,400 --> 00:07:48,440
+proteins ما معنى هذا الكلام؟ هلأ ال erythrocyte
+
+98
+00:07:48,440 --> 00:07:52,920
+sedimentation rate فحص ال ESR هو عبارة عن فحص
+
+99
+00:07:52,920 --> 00:07:59,680
+يستخدم used to يستخدم ليه؟ لحتى أعمل detection and
+
+100
+00:07:59,680 --> 00:08:04,790
+monitoring لمين؟ لل inflammatory responseإذا كان
+
+101
+00:08:04,790 --> 00:08:09,670
+في .. في الجسم inflammation أو tissue injury أو
+
+102
+00:08:09,670 --> 00:08:14,210
+infection تمام؟ ممكن أنا أعمل detection تنبؤ عن
+
+103
+00:08:14,210 --> 00:08:18,230
+وجود هاي ال .. ال infection أو ال inflammation أو
+
+104
+00:08:18,230 --> 00:08:21,950
+ال tissue injury ممكن أعمل monitoring أشوف الوضع
+
+105
+00:08:21,950 --> 00:08:28,160
+بتحسن مع الهلاج أو بيزيد سوء مثلا؟تمام؟ بعمل
+
+106
+00:08:28,160 --> 00:08:33,600
+monitoring مراقبة لحالة المريض من خلال فحص ال ESR
+
+107
+00:08:33,600 --> 00:08:37,560
+المريض اللي بيكون عنده إما inflammation أو tissue
+
+108
+00:08:37,560 --> 00:08:43,100
+injury أو حتى infection تمام؟ طيب ولكن هذا الفحص
+
+109
+00:08:43,100 --> 00:08:48,400
+is non specific يعني مثلا إذا كان نتيجة ال ESR
+
+110
+00:08:48,400 --> 00:08:52,160
+عالية بيكون يعني في عندي inflammation أو infection
+
+111
+00:08:52,160 --> 00:08:57,460
+أو tissue injury طيبخلاص أنا بعرف إنه في
+
+112
+00:08:57,460 --> 00:09:02,840
+inflammation لكن هل ب .. يعني بإمكاني أحدد وين ال
+
+113
+00:09:02,840 --> 00:09:07,100
+infection أو وين ال inflammation في القلب، في ال
+
+114
+00:09:07,100 --> 00:09:10,460
+kidney، في ال liver، في الدم؟ وين بالظبط؟ أنا
+
+115
+00:09:10,460 --> 00:09:15,390
+مابقدر أحددهاتمام؟ إذا لا أستطيع تحديد مكان ال
+
+116
+00:09:15,390 --> 00:09:18,150
+infection أو ال inflammation عشان هيك هو non
+
+117
+00:09:18,150 --> 00:09:23,590
+-specific مش متخصص فحص غير متخصص ولكن هو فحص لل
+
+118
+00:09:23,590 --> 00:09:28,910
+screening تمام؟ للتشييك لحتى أشيك لحتى أكشف أعمل
+
+119
+00:09:28,910 --> 00:09:33,510
+detection and monitoring لل inflammation و ال
+
+120
+00:09:33,510 --> 00:09:39,790
+tissue injury و طبعا يا بناتلأنه في حالة ال
+
+121
+00:09:39,790 --> 00:09:42,330
+inflammation و ال tissue injury و ال infection
+
+122
+00:09:42,330 --> 00:09:48,010
+بيحدث أو بيتم إنتاج بروتينات أو بيزيد تركيز
+
+123
+00:09:48,010 --> 00:09:52,850
+بروتينات في الدم، في ال plasma، بنسميها acute face
+
+124
+00:09:52,850 --> 00:09:57,010
+protein، تمام؟ بنسميها ال acute face protein طبعا
+
+125
+00:09:57,010 --> 00:10:01,990
+هذه ال acute face protein بيطلعب دور مهم جدا في
+
+126
+00:10:01,990 --> 00:10:08,580
+تسريع ترسيب ال RBCsطبعا فإذا وجود ال acute face of
+
+127
+00:10:08,580 --> 00:10:12,880
+protein دلالة على وجود acute face of inflammation
+
+128
+00:10:12,880 --> 00:10:18,580
+أو tissue injury أو infection إذا ESR ماله بيكون
+
+129
+00:10:18,580 --> 00:10:24,300
+قيمته عاليةطيب this procedure very simply consist
+
+130
+00:10:24,300 --> 00:10:29,500
+of allowing specific amount of blood to sit in
+
+131
+00:10:29,500 --> 00:10:33,000
+vertical position for a period of time يعني
+
+132
+00:10:33,000 --> 00:10:38,300
+وظيفتها ببساطة أو فكرة الفحص هذا ببساطة انه انا
+
+133
+00:10:38,300 --> 00:10:44,660
+اخلي عينة الدم انها تكون موجودة بشكل عمودي لمدة
+
+134
+00:10:44,660 --> 00:10:50,100
+زمنية معينة يعني one hour مثلا او عادة يعني عادة
+
+135
+00:10:50,190 --> 00:10:56,030
+نستخدمها يعني التوقيت one hour وطبعا أنا بقيس ال
+
+136
+00:10:56,030 --> 00:10:58,890
+distance اللي هي المسافة اللي قطعتها ال RBCs اللي
+
+137
+00:10:58,890 --> 00:11:04,210
+هي مسافة البلازمة تمام؟ بالmillimeter per hour إذا
+
+138
+00:11:04,210 --> 00:11:10,140
+الوحدة القياس هتكون millimeter per hourThe ESR is
+
+139
+00:11:10,140 --> 00:11:15,740
+affected by three factors ال ESR هذا بيتأثر بتلت
+
+140
+00:11:15,740 --> 00:11:22,820
+عوامل هناك تلاتة players بيلعبوا دور مهم جدا في
+
+141
+00:11:22,820 --> 00:11:29,040
+نتيجة ال ESR أو قيمة ال ESRأول إيش الارتروسايت
+
+142
+00:11:29,040 --> 00:11:32,220
+اللي .. اللي أصلا مفعول بيه أو اللي هي عبتصري لها
+
+143
+00:11:32,220 --> 00:11:36,140
+sedimentation أهم player عندى أيضا ال plasma
+
+144
+00:11:36,140 --> 00:11:40,660
+composition حكينا مكونات البلازمة البلازمة بروتين
+
+145
+00:11:40,660 --> 00:11:46,140
+ال acute face protein اللي بتظهر أو بيزيد تركيزها
+
+146
+00:11:46,140 --> 00:11:49,740
+في حالات ال inflammation و ال infection و ال
+
+147
+00:11:49,740 --> 00:11:54,800
+tissue injury طبعاهذه البروتينات موجودة في
+
+148
+00:11:54,800 --> 00:11:57,660
+البلازمة إذا المكونات البلازمة البلازمة
+
+149
+00:11:57,660 --> 00:12:05,100
+composition مهمة جدا لحتى يعني نشوف كيف بتأثر على
+
+150
+00:12:05,100 --> 00:12:08,700
+ال ESR أيضا ال mechanical و ال technical factors
+
+151
+00:12:08,700 --> 00:12:13,660
+عوامل يعني لها علاقة بشغلي كيف أنا جمعت العينة كيف
+
+152
+00:12:13,660 --> 00:12:16,780
+عملت لها mixing مع ال anticoagulant كيف اشتغلت
+
+153
+00:12:16,780 --> 00:12:20,300
+الفحص البيئة و الظروف اللي اشتغلت فيها الفحص هاي
+
+154
+00:12:20,300 --> 00:12:23,800
+ممكن يأثر على النتيجة راح نشوف كيفلاحقا في ال
+
+155
+00:12:23,800 --> 00:12:28,960
+slides التالية خلينا نشوف أول player عندي اللي هي
+
+156
+00:12:28,960 --> 00:12:34,720
+ال erythrocyte erythrocyte is a factor of chief
+
+157
+00:12:34,720 --> 00:12:38,240
+importance in determining the distance that the
+
+158
+00:12:38,240 --> 00:12:43,320
+RBCs fall تمام؟بحكيلي إنه ال .. ال .. ال
+
+159
+00:12:43,320 --> 00:12:46,300
+erythrocyte .. ال erythrocyte يعني هي عبارة عن
+
+160
+00:12:46,300 --> 00:12:52,500
+عامل مهم جدا في التأثير على نتيجة ال ESR ولعله إنه
+
+161
+00:12:52,500 --> 00:13:00,200
+أهم إشي بال RBCs هو حجمها ال size تبعها و ال mass
+
+162
+00:13:00,200 --> 00:13:05,520
+تبعتها تمام؟ هو اللي بيتحكم بمعدل سقوتها أو معدل
+
+163
+00:13:05,520 --> 00:13:12,210
+ترسيبها في الأنابيبthe larger the particle, the
+
+164
+00:13:12,210 --> 00:13:16,550
+faster its rate of fall طبعا يا بنات لو ال slides
+
+165
+00:13:16,550 --> 00:13:21,390
+عندكم المعلومة هي موجودة مختلفة فياريت تنتبهولها و
+
+166
+00:13:21,390 --> 00:13:24,950
+تعدلوها the larger the particle, the faster its
+
+167
+00:13:24,950 --> 00:13:29,090
+rate of fall طبعا يعني كل ما كبرت خلية ال RBC
+
+168
+00:13:29,090 --> 00:13:36,670
+بيسرع من سقوتها من معدل سقوتهاتمام طيب في عندي
+
+169
+00:13:36,670 --> 00:13:44,570
+أمراض ممكن تسبب ال RBCs size أكبر من الطبيعي وفي
+
+170
+00:13:44,570 --> 00:13:49,910
+عندي أمراض ممكن تتسبب بإنه ال RBCs يكون حجمها أقل
+
+171
+00:13:49,910 --> 00:13:55,450
+من الطبيعي فالأمراض اللي بتسببلي خلايا RBCs حجمها
+
+172
+00:13:55,450 --> 00:14:00,070
+أكبر من الطبيعي يعني larger particle هذا هيكون ال
+
+173
+00:14:00,070 --> 00:14:05,570
+ESR أشماله عندها عاليلأنه faster rate of fall،
+
+174
+00:14:05,570 --> 00:14:12,470
+تمام؟ طيب، أما إذا كانت ال .. ال erythrocyte حجمها
+
+175
+00:14:12,470 --> 00:14:17,930
+صغير فمعنها هذا the slower rate to fall طيب، خليني
+
+176
+00:14:17,930 --> 00:14:21,490
+أنشوف في الوضع الطبيعي، في الوضع الطبيعي بيحكيلي
+
+177
+00:14:21,490 --> 00:14:26,920
+إن ال normal blood في الدم الطبيعيThe RBCs remain
+
+178
+00:14:26,920 --> 00:14:32,060
+more or less separated. They are negatively
+
+179
+00:14:32,060 --> 00:14:37,020
+charged and therefore rebel each other خلايا ال
+
+180
+00:14:37,020 --> 00:14:42,880
+RBCs موجودة على سطحها carbohydrate اسمها سيالك
+
+181
+00:14:42,880 --> 00:14:46,020
+أسد، نوع من أنواع ال carbohydrate اللي اسمه سيالك
+
+182
+00:14:46,020 --> 00:14:50,980
+أسديكسب خلية ال RBCs، تمام يا بنات؟ يكسبها
+
+183
+00:14:50,980 --> 00:14:55,160
+negative charge، اذا خلايا ال RBCs مشحونة من
+
+184
+00:14:55,160 --> 00:15:01,280
+الخارج بشحنة سالبة، negative charge، تمام؟ طيب
+
+185
+00:15:01,930 --> 00:15:05,690
+فخلايا الاربسيز ال negative charge اللي على سطحها
+
+186
+00:15:05,690 --> 00:15:10,370
+بتخليها separated عن بعضها البعض يعني في على
+
+187
+00:15:10,370 --> 00:15:14,170
+مسافات ثابتة من بعضها البعض او في متباعدة هي بتكون
+
+188
+00:15:14,170 --> 00:15:19,850
+مالها متباعدة في ما بينها قوة تنافر repulsion
+
+189
+00:15:19,850 --> 00:15:24,530
+forces ال repulsion forces هذه قوات تنافر بينشأ
+
+190
+00:15:24,530 --> 00:15:28,570
+عنها فرق جهد ما بين الخلايا بسميه zeta potential
+
+191
+00:15:29,200 --> 00:15:33,740
+هذا فرق الجهد اللي اسمه zeta potential ناتج من ال
+
+192
+00:15:33,740 --> 00:15:37,420
+revolution forces اللي هو قوات التنافر والشحنات
+
+193
+00:15:37,420 --> 00:15:41,060
+السالبة اللي موجودة على سطح خلايا ال RBCs فهذا
+
+194
+00:15:41,060 --> 00:15:45,440
+الفرق الجهد بيخلي الخلايا أشمالها separated بعيدة
+
+195
+00:15:45,440 --> 00:15:50,880
+عن بعضها البعضطبعا؟ طيب خلّينا بس نشوف نستعرض صورة
+
+196
+00:15:50,880 --> 00:15:55,640
+و لحتى نعرف إيش يعني إن ال RBC بتكون negatively
+
+197
+00:15:55,640 --> 00:15:58,960
+charged كيف بتكون متباعدها؟ يعني بتكون على سطحها
+
+198
+00:15:58,960 --> 00:16:03,560
+شحنة سالبة هذا بيخليها على مسافة معينة من الخلايا
+
+199
+00:16:03,560 --> 00:16:06,700
+المجاورة لإلها إذا الخلايا ال RBC ما بتكونش إيش
+
+200
+00:16:06,700 --> 00:16:09,860
+ملتصقة مع بعض البعض في العينة أو حتى في ال
+
+201
+00:16:09,860 --> 00:16:14,560
+circulation في الدمتمام؟ بتكون على مسافة معينة من
+
+202
+00:16:14,560 --> 00:16:20,640
+بعضها البعض طبعا هذه الخلايا في مابينها قوة تنافر
+
+203
+00:16:20,640 --> 00:16:24,760
+و أيضا zeta potential فرق جهد اسمه zeta potential
+
+204
+00:16:24,760 --> 00:16:30,400
+بي .. بي .. يعني بيضمن لإن ال RBCs تكون اشمالها
+
+205
+00:16:30,400 --> 00:16:37,450
+متباعدة عن بعضها البعضطبعا؟ طيب فاللي بيصير في
+
+206
+00:16:37,450 --> 00:16:42,790
+الترسيب أنا عندى نسبة البروتينات قليلة جدا فى الدم
+
+207
+00:16:42,790 --> 00:16:45,810
+يعنى وضعه الطبيعى انها تكون قليلة ال acute facet
+
+208
+00:16:45,810 --> 00:16:49,270
+protein طبعا؟ لإن فى الوضع الطبيعى احنا بنحكى يعنى
+
+209
+00:16:49,270 --> 00:16:53,990
+فش inflammation يعنى فش infection طبعا؟ فبالتالي
+
+210
+00:16:53,990 --> 00:16:58,850
+فش عندي acute facet protein بتركيز عالى عندي الوضع
+
+211
+00:16:58,850 --> 00:17:02,820
+الطبيعى او التركيز الطبيعى من هى البروتيناتفإيش
+
+212
+00:17:02,820 --> 00:17:06,160
+بتروح بتعمل هاي البروتينات؟ خلّينا نشوف في الوضع
+
+213
+00:17:06,160 --> 00:17:09,960
+الطبيعي الحد
+
+214
+00:17:09,960 --> 00:17:13,640
+الأدنى من البروتينات هذه أو الحد الطبيعي بيجي
+
+215
+00:17:13,640 --> 00:17:17,760
+وبيلتف حوالين ال RBC زي هيك وإحنا في ال tube وإحنا
+
+216
+00:17:17,760 --> 00:17:21,300
+مجلسينها بشكل عمودي لمدة ساعة بيجي ال ..
+
+217
+00:17:21,300 --> 00:17:25,000
+البروتينات و هي بتعادل شحنة ال erythrocyte بهذا
+
+218
+00:17:25,000 --> 00:17:30,070
+الشكلطبعا طيب بما أنها عادلة الشحنة إذا انقلت ال
+
+219
+00:17:30,070 --> 00:17:34,250
+rebellion forces ما بين ال RBCs إذا ال RBCs هتقترب
+
+220
+00:17:34,250 --> 00:17:39,050
+من بعضها البعض هذا .. هذا يعني number one طبعا
+
+221
+00:17:39,050 --> 00:17:44,510
+number two المسافة ما بين ال RBCs و أختها انشغلت
+
+222
+00:17:44,510 --> 00:17:49,190
+ببروتينات هذا رح يقدي إلى إيش crowding تزاهم ما
+
+223
+00:17:49,190 --> 00:17:54,400
+بين ال RBCsفيعني مدام صار في عندي crowding أو
+
+224
+00:17:54,400 --> 00:17:57,860
+تزاحم ما بين ال RBC أو تقارب ما بينها خلينا نسمي
+
+225
+00:17:57,860 --> 00:18:01,920
+تقارب ما بينهم اذا صار في تجمعات من .. ما بين ال
+
+226
+00:18:01,920 --> 00:18:08,360
+RBCs اذا طالما صار في عندي تجمعات من ال RBCs يعني
+
+227
+00:18:08,360 --> 00:18:13,120
+صار في عندها .. عندي larger particle كبير حجمها
+
+228
+00:18:13,120 --> 00:18:19,150
+اذا تسقط تمام بيصير لها sedimentationطيب، هذا
+
+229
+00:18:19,150 --> 00:18:23,890
+الوضع الطبيعي احنا بنحكي، طبعا؟ طيب خلينا نشوف لو
+
+230
+00:18:23,890 --> 00:18:29,660
+.. لوفي عندي certain disease لو في حالة certain
+
+231
+00:18:29,660 --> 00:18:34,720
+disease ال plasma protein concentration و may be
+
+232
+00:18:34,720 --> 00:18:39,900
+altered تركيز ال plasma protein هيتغير هيزيد عندي
+
+233
+00:18:39,900 --> 00:18:44,760
+مين؟ هيزيد عندي ال .. ال acute face protein في
+
+234
+00:18:44,760 --> 00:18:47,160
+حالات ال infection و ال inflammation و ال tissue
+
+235
+00:18:47,160 --> 00:18:52,100
+injury تمام؟ اذا هاي ال acute face protein اللي
+
+236
+00:18:52,100 --> 00:18:58,020
+زادت عن حدهازادت على الحد الطبيعي، إيش هتعمل؟
+
+237
+00:18:58,020 --> 00:19:02,000
+هتعمللي reduction في ال negative charge أكتر و
+
+238
+00:19:02,000 --> 00:19:07,560
+هتعمللي تجمعات أكتر و هتقلل ال zeta potential اللي
+
+239
+00:19:07,560 --> 00:19:11,640
+ما بين الخلايا أكتر و تقرب الخلايا ما بينها و ما
+
+240
+00:19:11,640 --> 00:19:16,100
+بين بعضها أكتر، تمام؟ فبالتالي هذا إيش هيعمللي؟
+
+241
+00:19:16,100 --> 00:19:21,820
+Larger massكتلة أكبر من الخلايا تسقط أسرع، إذا أنا
+
+242
+00:19:21,820 --> 00:19:27,260
+في عندي ESR أعلى، يعني أنا ترسيب عندي في .. بيصير
+
+243
+00:19:27,260 --> 00:19:31,540
+increase in the velocity، في سرعة الترسيب، تمام؟
+
+244
+00:19:31,540 --> 00:19:34,500
+increase sedimentation velocity أو increase
+
+245
+00:19:34,500 --> 00:19:39,600
+sedimentation rate، معدل الترسيب أشماله بيصير ..
+
+246
+00:19:39,600 --> 00:19:46,490
+بيصير أعلى، تمام؟ بيصير أعلىنتيجة إنه البروتينات
+
+247
+00:19:46,490 --> 00:19:53,110
+زادت ال acute phase protein فأدت إلى تقارب أكثر ما
+
+248
+00:19:53,110 --> 00:19:57,430
+بين الخلايا و تجمعات أكتر هاي تجمعات الخلايا يا
+
+249
+00:19:57,430 --> 00:20:01,450
+بنات بسميها roll-off formation إيش بسميها؟ roll
+
+250
+00:20:01,450 --> 00:20:04,790
+-off formation ورح نشرح إيش يعني roll-off
+
+251
+00:20:04,790 --> 00:20:09,910
+formation خلال ال slides القادمة، تمام؟ بس خليني
+
+252
+00:20:09,910 --> 00:20:18,350
+أشرحلكم على هاي ال slideخلّيني أشوف خلايا متباعدة
+
+253
+00:20:18,350 --> 00:20:24,190
+عن بعضها البعض مع عملات تجمعات، تمام؟ فيعني زي
+
+254
+00:20:24,190 --> 00:20:29,490
+السيارات كأنها بتقطع مسافة أو بسرعة متباعدة مع
+
+255
+00:20:29,490 --> 00:20:35,080
+بعضها البعض، تمام؟تخيل .. تخيليها سيارات متباعدة
+
+256
+00:20:35,080 --> 00:20:40,240
+يعني هي زي ال RBCs المتباعدة، طبعا؟ فمعدل سقوط هذه
+
+257
+00:20:40,240 --> 00:20:43,960
+ال RBCs نتيجة إنها متباعدة يعني مافيش عندي ..
+
+258
+00:20:43,960 --> 00:20:50,500
+مافيش عندي تجمعات، طبعا؟ مافيش عندي تجمعات لل
+
+259
+00:20:50,500 --> 00:20:54,400
+RBCs، إذن هل هتسقط بسرعة؟ لأ، يعني هت .. ممكن تاخد
+
+260
+00:20:54,400 --> 00:20:59,540
+الوضع الطبيعي للسقوطنتيجة التباعد الطبيعي ونتيجة
+
+261
+00:20:59,540 --> 00:21:03,980
+وجود ال acute face protein بالمعدل الطبيعي تمام؟
+
+262
+00:21:03,980 --> 00:21:07,720
+زادت ال acute face protein يعني تقاربت ال RBCs
+
+263
+00:21:07,720 --> 00:21:12,280
+أكتر تمام؟ فصار في عندي ash ال .. ال .. ال .. زي
+
+264
+00:21:12,280 --> 00:21:15,500
+هنا السيارات مثلا صارت متقاربة من بعضها البعض
+
+265
+00:21:15,500 --> 00:21:19,860
+تمام؟ طيب فإذا طالما أنها تقاربت إذا صار في عندي
+
+266
+00:21:19,860 --> 00:21:25,530
+نسبة يعني ..معدل سقوطها على طريق ما، طريق يعني
+
+267
+00:21:25,530 --> 00:21:30,350
+نازل بنزول، هيكون أسرع، صح؟ تمام، نفس الفكرة هنا
+
+268
+00:21:30,350 --> 00:21:34,970
+طيب، لو كانت الخلايا متقاربة أكثر فأكثر فأكثر،
+
+269
+00:21:34,970 --> 00:21:38,610
+فتجمعت يعني، فهتكون زي ك .. زي ك .. زي كأنها قطار
+
+270
+00:21:38,610 --> 00:21:43,240
+ومسرعفي النزول و زي .. هيك عملالي تجمعات هذا
+
+271
+00:21:43,240 --> 00:21:48,340
+التجمعات أنا بسميها roll-off formation تمام؟
+
+272
+00:21:48,340 --> 00:21:51,800
+بسميها roll-off formation طب إيش يعني roll-off
+
+273
+00:21:51,800 --> 00:21:56,180
+formation؟ يعني إنه الخلايا ال RBC بتكون متجمعة
+
+274
+00:21:56,180 --> 00:22:02,240
+على بعضها البعض بشكل likestack coins إيش يعني like
+
+275
+00:22:02,240 --> 00:22:08,520
+stack coins؟ يعني زي القطع المعدنية اللي منزلقة
+
+276
+00:22:08,520 --> 00:22:13,020
+على بعضها البعض مثل القطع المعدنية اللي منزلقة على
+
+277
+00:22:13,020 --> 00:22:16,840
+بعضها البعض تخيلي أنت عندك صفة شواكل زي هيك و جيتي
+
+278
+00:22:16,840 --> 00:22:20,880
+ده حرشتيهم و منزلقوا على بعضهم البعض هذا بسميه شكل
+
+279
+00:22:20,880 --> 00:22:23,480
+ال roll-off formation نفس الشيء الخلايا بتاخد هذا
+
+280
+00:22:23,480 --> 00:22:29,020
+الشكل خليني هنا مثلا أجيأورجيكم إياه على الصور
+
+281
+00:22:29,020 --> 00:22:39,440
+لحتى تشوفوا كيف إيش يعني له low formation تمام؟
+
+282
+00:22:39,440 --> 00:22:45,690
+هنشوف الصور هناRouleau formation هاي شايفين؟ خلايا
+
+283
+00:22:45,690 --> 00:22:50,130
+ال RBCs تأخد شكل stacking coins زي كأنها قطع
+
+284
+00:22:50,130 --> 00:22:53,110
+معدنية ومنزلقة على بعض البعض زي صفتة الشواكل اللي
+
+285
+00:22:53,110 --> 00:22:58,250
+انت بتدحرجيهم تحت ال microscope بتكون بهذا الشكل
+
+286
+00:22:58,250 --> 00:23:01,790
+شايفين؟ هاي هنا كمان أيضا تحت ال microscope هذا
+
+287
+00:23:01,790 --> 00:23:05,350
+بسميه Rouleau formation هذا Rouleau formation هذا
+
+288
+00:23:05,350 --> 00:23:09,930
+Rouleau formation تمام؟ إذن هذه صورة تقريبيةو هاي
+
+289
+00:23:09,930 --> 00:23:13,750
+صورة تحت ال microscope له formation زي ال RBC's
+
+290
+00:23:13,750 --> 00:23:17,710
+منظلقة على بعض هالبعض مثل ال stack coins تمام؟
+
+291
+00:23:17,710 --> 00:23:26,100
+like stack coins طيبهيك بنكون شرحنا فكرة الفحص،
+
+292
+00:23:26,100 --> 00:23:29,580
+عرفنا إيش دور الاريثروسايت في ال .. ال
+
+293
+00:23:29,580 --> 00:23:34,980
+sedimentation، طبعا، حجمها ووجود لبروتينة ..
+
+294
+00:23:34,980 --> 00:23:39,100
+وشحناتها، ال negative charge اللي حواليها، التباعد
+
+295
+00:23:39,100 --> 00:23:45,670
+ما بينها، وجود لبروتيناتبنسبة معينة ممكن تقرب ما
+
+296
+00:23:45,670 --> 00:23:50,690
+بينها يصير في عندي تجمعات roll-off formation يعني
+
+297
+00:23:50,690 --> 00:23:55,830
+صار في عندي larger particle فهيسقط بشكل أسرع
+
+298
+00:23:55,830 --> 00:23:59,190
+وبالتالي هيصير في عندي sedimentation rate هذا
+
+299
+00:23:59,190 --> 00:24:02,670
+بالوضع الطبيعي هيكون ال sedimentation rate طبيعي
+
+300
+00:24:02,670 --> 00:24:05,630
+ونسبة أيضا البروتينات اللي ساهمت في التقارب ما بين
+
+301
+00:24:05,630 --> 00:24:09,210
+ال RBC و ال roll-off formation هتكون طبيعيةفي حالة
+
+302
+00:24:09,210 --> 00:24:11,970
+disease إذا أنا في عندي plasma protein
+
+303
+00:24:11,970 --> 00:24:15,830
+concentration عالي يعني acute face protein عالي
+
+304
+00:24:15,830 --> 00:24:19,950
+فبالتالي هيكون عندي .. هيصير في reduction في ال
+
+305
+00:24:19,950 --> 00:24:23,010
+negative charge، هيكون في تقارب ما بين ال RBCs
+
+306
+00:24:23,010 --> 00:24:27,370
+أكتر، له low formation أكتر، larger mass، increase
+
+307
+00:24:27,370 --> 00:24:31,190
+sedimentation rate، تمام؟و أيضا في حالة ال
+
+308
+00:24:31,190 --> 00:24:35,930
+agglutination يا بنات تجلط الدم او تجمع خلايا الدم
+
+309
+00:24:35,930 --> 00:24:40,930
+مع بعضها البعض و نتيجة تغير شكلها او تغير سطحها
+
+310
+00:24:40,930 --> 00:24:47,190
+هذا ايضا ممكن انه يعملي increase RBCs ماسقى يزود
+
+311
+00:24:47,190 --> 00:24:51,370
+من كتلة ال RBCs فيسرع سقوتها يعني هلأ انا طبعا لو
+
+312
+00:24:51,370 --> 00:24:57,390
+بدي ارمي يعني أسقط صخرة من علو ولا أسقط حجر صغير
+
+313
+00:24:57,390 --> 00:25:02,260
+الصخرة هتنزل أسرع نتيجة كتلتهاأعلى، تمام؟ هاي هنا
+
+314
+00:25:02,260 --> 00:25:07,160
+بس الفكرة ف ال RBCs larger particle faster rate to
+
+315
+00:25:07,160 --> 00:25:14,450
+fall طيبال .. ال macro site .. macro معناها كبير
+
+316
+00:25:14,450 --> 00:25:19,810
+حجمها كبير very .. يعني large size site خلية
+
+317
+00:25:19,810 --> 00:25:24,630
+الخلية اللي حجمها كبير tend to settle more rapidly
+
+318
+00:25:24,630 --> 00:25:29,350
+than .. mean than micro site micro يعني صغير و
+
+319
+00:25:29,350 --> 00:25:33,100
+site خلية إذا الخلايا اللي حجمها كبيرنتيجة ان ال
+
+320
+00:25:33,100 --> 00:25:36,380
+mass تبعتها كبيرة و ال size تبعتها كبير حكينا
+
+321
+00:25:36,380 --> 00:25:40,340
+اشمالها fall او settle او sedimentation بيصيرلها
+
+322
+00:25:40,340 --> 00:25:44,800
+sedimentation more rapidly اسرع من ال micro site
+
+323
+00:25:44,800 --> 00:25:51,020
+في حالة ال anisocytosis و الbuchelocytosis رح يصير
+
+324
+00:25:51,020 --> 00:25:57,480
+في عندي reduce ل ال RBCs aggregation فبالتالي هيقل
+
+325
+00:25:57,480 --> 00:26:02,980
+ال ESRإيش يعني أنيسو سايتوسز و أبو كيلو سايتوسز؟
+
+326
+00:26:02,980 --> 00:26:06,340
+هلأ الانيسو سايتوسز يعني variation في ال cell size
+
+327
+00:26:06,340 --> 00:26:12,240
+يعني تباين في حجم الخلايا، تمام؟ تباين في حجم
+
+328
+00:26:12,240 --> 00:26:16,740
+الخلايايعني الخلايا حجمها كبير، خلايا وسط، خلايا
+
+329
+00:26:16,740 --> 00:26:22,780
+صغيرة، هكذا يعني طيب بوكيلوسايتوسز تباين في شكل
+
+330
+00:26:22,780 --> 00:26:26,680
+الخلايا في shape للخلايا يعني عندي خلايا شكلها
+
+331
+00:26:26,680 --> 00:26:30,180
+الشكل الطبيعي اللي هي bacon cave او discotheque في
+
+332
+00:26:30,180 --> 00:26:34,360
+عندي خلايا spherocyte شكلها كوراوي في خلايا شكلها
+
+333
+00:26:34,360 --> 00:26:39,280
+شكل ال sickle cell من جلي، تمام؟ فهذا التباين في
+
+334
+00:26:39,280 --> 00:26:45,000
+شكل الخلايا بسميه بوكيلوسايتوسزطيب، هلأ تجمعات
+
+335
+00:26:45,000 --> 00:26:49,720
+الخلايا لازم تكون الخلايا .. يعني لحتى تحدث هاي
+
+336
+00:26:49,720 --> 00:26:52,200
+التجمعات، لحتى يحدث ال roll-off formation أو ال
+
+337
+00:26:52,200 --> 00:26:56,780
+RBC aggregation هذا لازم ان الخلايا تكون uniform،
+
+338
+00:26:56,780 --> 00:27:00,540
+منتظمة الشكل والحجم عشان انها تتجمع مع بعضها
+
+339
+00:27:00,540 --> 00:27:03,520
+البعض، تلتصق زي ال stack coins زي ما شوفنا في
+
+340
+00:27:03,520 --> 00:27:09,220
+الصورة من شوي و تسقط مع بعضها البعضكلن يعني يتجمع
+
+341
+00:27:09,220 --> 00:27:14,320
+على شكلته طبعا ال RBCs لازم تكون يعني بتشبه بعضها
+
+342
+00:27:14,320 --> 00:27:19,520
+بالشكل و الحجم علشان ايه اشملها انه يعني يصيرها
+
+343
+00:27:19,520 --> 00:27:23,300
+sedimentation مع بعض تصيرها aggregation و بعدها
+
+344
+00:27:23,300 --> 00:27:28,070
+sedimentation مع بعض احنا مثلا لما بنصاحب ناسمن
+
+345
+00:27:28,070 --> 00:27:31,610
+صاحبهم بيشبهونا مش شرط بالشكل أو بالحجم ممكن
+
+346
+00:27:31,610 --> 00:27:37,590
+يكونوا بيشبهونا باصطفات تمام؟ فإنه يعني احنا بيكون
+
+347
+00:27:37,590 --> 00:27:42,290
+في تواؤمة بينهم على نفس الفكرة ال RBCs لازم انه
+
+348
+00:27:42,290 --> 00:27:45,810
+علشان يصير لها aggregation و low formation تكون ..
+
+349
+00:27:45,810 --> 00:27:52,010
+تكون على الأقل منتظمة الشكل و الحجمطبعا طيب لكن
+
+350
+00:27:52,010 --> 00:27:55,210
+إذا كان في عندي تباين في الحجم أو تباين في الشكل
+
+351
+00:27:55,210 --> 00:28:00,630
+هذا راح يقدي إلى inhibition أو reduce the ability
+
+352
+00:28:00,630 --> 00:28:06,630
+of RBCs يقلل من قدرة خلايا ال RBCs على إنها تعمل
+
+353
+00:28:06,630 --> 00:28:12,790
+aggregation تعمل larger particle ويحلهافخلايا ال
+
+354
+00:28:12,790 --> 00:28:16,450
+RBCs راح تسقط يعني على الأغلب فرادة أو هي و هي
+
+355
+00:28:16,450 --> 00:28:22,070
+تجمعات قليلة صغيرة جدا فهذا يقلل الإيسار طبعا؟
+
+356
+00:28:22,070 --> 00:28:23,710
+معدل الترسيب
+
+357
+00:28:26,070 --> 00:28:30,630
+طيب RBCs هنا بيشرحلي إيش معناة كلمة anisocytosis
+
+358
+00:28:30,630 --> 00:28:33,650
+and bukylocytosis بتقلل ال aggregation و ال low
+
+359
+00:28:33,650 --> 00:28:37,930
+formation و بتقلل ال ESR بيحكيلك ال RBCs should
+
+360
+00:28:37,930 --> 00:28:41,730
+show an alteration in shape يعني شكلها يعني متغير
+
+361
+00:28:41,730 --> 00:28:46,600
+زي ال sickle cellخلايا المنجالية والكوراوية طبعا
+
+362
+00:28:46,600 --> 00:28:51,200
+ما .. ما .. يعني لا تتمكن أو لا تفتقد القدرة علي
+
+363
+00:28:51,200 --> 00:28:54,740
+إنها تعمل لي aggregation أو low formation فبالتالي
+
+364
+00:28:54,740 --> 00:28:58,240
+الارترو سايت sedimentation rate أشماله بيكون
+
+365
+00:28:58,240 --> 00:29:02,580
+decreased في حالة الانيسو سايتوسز والكيلو سايتوسز
+
+366
+00:29:04,000 --> 00:29:09,440
+تمام، في حالة ال severe anemia، anemia فقر دم يعني
+
+367
+00:29:09,440 --> 00:29:14,420
+في .. severe، كتير .. يعني كتير متقدمة .. متقدمة
+
+368
+00:29:14,420 --> 00:29:18,760
+الحالة، يعني كتير anemia، مستوى ال anemia كتير،
+
+369
+00:29:18,760 --> 00:29:24,120
+تمام؟ طيب، the ESR is markedly elevated، بيكون
+
+370
+00:29:24,120 --> 00:29:28,840
+عالي، ليش؟ لأن anemia يعني نقص homoglobin، يعني
+
+371
+00:29:28,840 --> 00:29:35,930
+نقص RBCsRed blood cell قليلة بالنسبة للبلازمة فلما
+
+372
+00:29:35,930 --> 00:29:40,270
+بتسقط اشملها أول حاجة مابدهاش وقت لتسقط لإن عددها
+
+373
+00:29:40,270 --> 00:29:47,330
+قليل تاني إشي لإن نسبة البلازمة بيأخد حيث أكبر من
+
+374
+00:29:47,330 --> 00:29:54,340
+الخلايا فهذارح يقدّي إلى إن ال ESR يزيد، إذا ال
+
+375
+00:29:54,340 --> 00:29:58,380
+concentration of RBCs قليل في حالة الانيميا، تركيز
+
+376
+00:29:58,380 --> 00:30:02,520
+الخلايا، عدد الخلايا قليل في ال plasma فال
+
+377
+00:30:02,520 --> 00:30:05,860
+aggregation و ال role of formation هيزيد، تمام؟
+
+378
+00:30:06,580 --> 00:30:12,060
+بالإضاءة يعني هتسقط بسرعة لإنه عددها قليل و لإنه
+
+379
+00:30:12,060 --> 00:30:16,540
+تجمعها هيكون بسرعة و لإنه نسبة البلازمة بالنسبة
+
+380
+00:30:16,540 --> 00:30:22,060
+للخلايا أعلى فهذه أسباب كافية لإنه اتخليلي ال ESR
+
+381
+00:30:22,060 --> 00:30:26,840
+markedly elevated طبعا؟ طيب في حالة ال
+
+382
+00:30:26,840 --> 00:30:33,000
+polycythemiaESR is generally decreased طبعا في
+
+383
+00:30:33,000 --> 00:30:36,860
+حالة البوليسيثيميا ال ESR is generally decreased
+
+384
+00:30:36,860 --> 00:30:41,440
+بيقل ال ESR أول إشي طبعا إحنا حكينا إنه
+
+385
+00:30:41,440 --> 00:30:46,120
+البوليسيثيميا يعني زيادة في خلايا الدم ال RBCs
+
+386
+00:30:46,120 --> 00:30:49,500
+طبعا نعكس ال concentration هنا في حالة
+
+387
+00:30:49,500 --> 00:30:54,410
+البوليسيثيميا إيش معله هيكون increaseتمام؟ يعني ال
+
+388
+00:30:54,410 --> 00:30:56,950
+aggregation و ال role of formation اشماله هيكون
+
+389
+00:30:56,950 --> 00:31:00,350
+decrease خلايا يعني بدها وقت لا تتجمع مع بعض على
+
+390
+00:31:00,350 --> 00:31:04,550
+بعض أو بدها وقت لا تسقط لإن عددها كبير تمام؟ و
+
+391
+00:31:04,550 --> 00:31:08,430
+أيضا في حق البوليسايثيميا احنا حكينا إنه نوع من
+
+392
+00:31:08,430 --> 00:31:13,410
+أنواعها أحيانا بيكون cancer تمام؟ خلية الأم لل
+
+393
+00:31:13,410 --> 00:31:17,790
+RBCs في ال bone marrow بيصير فيها mutation بيصير
+
+394
+00:31:17,790 --> 00:31:22,030
+تنقسم بشكل مجنون فبتعطيني RBCs كتير على ال
+
+395
+00:31:22,030 --> 00:31:27,060
+circulationتمام؟ في حالات ال .. ال cancer بشكل عام
+
+396
+00:31:27,060 --> 00:31:33,620
+بتزيد لزوجة ال .. البلازمة تمام؟ بتزيد ال
+
+397
+00:31:33,620 --> 00:31:40,360
+viscosity للبلازمة بتزيد لزوجة البلازمة فال RBCs
+
+398
+00:31:40,360 --> 00:31:46,960
+اللي عددها كبير high count of RBCs تمام؟ لما تيجي
+
+399
+00:31:46,960 --> 00:31:54,230
+تسقط في سائل لزج هتاخد وقت أكبر يعني هتسقط أبطأإذا
+
+400
+00:31:54,230 --> 00:32:00,450
+ال ESR إيش ماله؟ decrease تمام؟ هيكون هذا معناه ال
+
+401
+00:32:00,450 --> 00:32:03,470
+.. ال .. انه ال ESR decrease في حالة ال
+
+402
+00:32:03,470 --> 00:32:09,470
+polycythemia لو أنا جبت كورةتمام و حطيتها في tube
+
+403
+00:32:09,470 --> 00:32:14,450
+فيه ميه أسقطتها في ال tube فيه ميه و بعدين جبت كرة
+
+404
+00:32:14,450 --> 00:32:17,510
+تانية نفس الحجم و نفس الشكل و أسقطتها في ال tube
+
+405
+00:32:17,510 --> 00:32:22,150
+لنفترض مثلا فيه زيت أو glycerol أو سائل لزج مين
+
+406
+00:32:22,150 --> 00:32:26,590
+هيكون سقوط ال RBC او سقوط القرة اللي معايا أسرع
+
+407
+00:32:26,590 --> 00:32:30,110
+طبعا أكيد في ال .. في ال .. ال tube اللي فيه ميه
+
+408
+00:32:30,970 --> 00:32:35,010
+طبعا و احنا عندنا ال plasma أصلا 90% منها ماء
+
+409
+00:32:35,010 --> 00:32:41,070
+water طبعا فالمفروض أنه سقوتها أسرع في الماء لكن
+
+410
+00:32:41,070 --> 00:32:46,170
+لو زادت لزوجة ال plasma هذه فبالتالي هيصير سقوط ال
+
+411
+00:32:46,170 --> 00:32:53,130
+RBC's أبطأ هاي فكرتهاطبعا نيجي لل second player
+
+412
+00:32:53,130 --> 00:32:57,750
+اللي بيلعب دور مهم بال ESR أيضا ل plasma
+
+413
+00:32:57,750 --> 00:33:02,510
+composition the plasma composition مكونات البلازما
+
+414
+00:33:02,510 --> 00:33:06,970
+is a most important factor مهم يعني عامل لا يقل
+
+415
+00:33:06,970 --> 00:33:11,570
+أهميته عن ال RBCs في ال determining لل ESR لحتى
+
+416
+00:33:11,570 --> 00:33:17,320
+أنا أعرف قيمة ال ESRأو أقيص ال ESR رولوه و
+
+417
+00:33:17,320 --> 00:33:20,660
+Aggregation of RBCs are controlled primarily by
+
+418
+00:33:20,660 --> 00:33:23,940
+the level of acute phosphoprotein احنا شرحناها هاي
+
+419
+00:33:23,940 --> 00:33:27,880
+المعلومة حكينا انه الـRolo Formation و ال
+
+420
+00:33:27,880 --> 00:33:31,760
+Aggregation لل RBCs تقارب ال RBCs وتجمعها وتعمل
+
+421
+00:33:31,760 --> 00:33:36,920
+رولو Formation عشان تصير كتلة خلايا وتسقط أسرعطبعا
+
+422
+00:33:36,920 --> 00:33:40,340
+هذا محكم بمين؟ بال level of acute face protein
+
+423
+00:33:40,340 --> 00:33:43,600
+لنفترق .. طبعا ال acute face protein هي ماعطيني
+
+424
+00:33:43,600 --> 00:33:49,880
+مثال عليها fibrinogen, α1-globulin و α2-globulin
+
+425
+00:33:49,880 --> 00:33:54,660
+طبعا هذه كلها؟ لأ طبعا مش كلها مش ال .. مش كل ال
+
+426
+00:33:54,660 --> 00:33:57,480
+acute face protein هاي في acute face protein
+
+427
+00:33:57,480 --> 00:34:03,770
+ماذكروها ولكن هذه مثال عليهاطبعا هاي ال acute
+
+428
+00:34:03,770 --> 00:34:07,110
+facial protein حكينا إنها بتزيد في حالات tissue
+
+429
+00:34:07,110 --> 00:34:15,090
+injury, inflammation, infection طبعا طيب و بالتالي
+
+430
+00:34:15,090 --> 00:34:21,030
+إذا زادت نسبة هاي البروتينات فهتسرع طبعا إنها
+
+431
+00:34:21,030 --> 00:34:23,630
+هتعادل أول إش تقلل من ال negative charge اللي
+
+432
+00:34:23,630 --> 00:34:28,150
+موجودة حوالين ال RBCs فبالتالي هتقلل من ال
+
+433
+00:34:28,150 --> 00:34:32,860
+repulsion forcesفهتقلل من ال zeta potential يعني
+
+434
+00:34:32,860 --> 00:34:36,880
+المسافة ما بين ال RBCs هتقل و ايضا هي هتشغل
+
+435
+00:34:36,880 --> 00:34:41,120
+المسافة ما بين ال RBCs يعني هتحدث تقارب اكبر ل ال
+
+436
+00:34:41,120 --> 00:34:45,380
+RBCs يعني aggregation يعني rollout formation يعني
+
+437
+00:34:45,380 --> 00:34:50,440
+سقوط سريع لل RBCs طبعا؟ طيب خلينا بس نحكي هاي
+
+438
+00:34:50,440 --> 00:34:54,000
+الفقرة مهمة جدا يا بنات ايش .. ايش بتحكيلنا هاي
+
+439
+00:34:54,000 --> 00:35:00,680
+الفقرة؟as the concentration of a protein increases
+
+440
+00:35:00,680 --> 00:35:06,940
+كل ما زادت تركيز البروتينات في الدم أو في البلازمة
+
+441
+00:35:06,940 --> 00:35:11,680
+فبالتالي the viscosity of a plasma أيضا increase
+
+442
+00:35:11,680 --> 00:35:15,500
+طبعا as the concentration of a protein increases
+
+443
+00:35:15,500 --> 00:35:18,740
+so does the viscosity of a plasma يعني كل ما بتزيد
+
+444
+00:35:18,740 --> 00:35:22,960
+البروتينات تركيز البروتينات بيزيد لزوجة البلازمة
+
+445
+00:35:22,960 --> 00:35:29,850
+طبعاطيب فبالتالي although بالرغم من أن زيادة ال
+
+446
+00:35:29,850 --> 00:35:33,650
+plasma viscosity will tend to inhibit the fall of
+
+447
+00:35:33,650 --> 00:35:37,490
+RBCs and the increase in plasma protein are
+
+448
+00:35:37,490 --> 00:35:41,170
+generally those which cause low and aggregation of
+
+449
+00:35:41,170 --> 00:35:44,690
+RBCs which affect the ESR more greatly than the
+
+450
+00:35:44,690 --> 00:35:48,930
+increase in plasma viscosityهلأ صح إن زيادة
+
+451
+00:35:48,930 --> 00:35:54,830
+البروتينات في البلازمة ممكن إنها تزيد لزوجة
+
+452
+00:35:54,830 --> 00:36:01,250
+البلازمة، تمام؟ ولكنممكن إنها تزيد لزوجة البلازمة
+
+453
+00:36:01,250 --> 00:36:09,650
+ولكن يعني .. يعني بغض النظر إنها لو زادت لزوجة
+
+454
+00:36:09,650 --> 00:36:13,650
+البلازمة فبالتالي يعني إحنا عارفين إن إذا زادت
+
+455
+00:36:13,650 --> 00:36:17,950
+اللزوجة هي .. هي .. هي .. هيعمل إعاقة أو تبطيء
+
+456
+00:36:17,950 --> 00:36:24,200
+لسقوط خلايا الدم، تمام؟طيب، هل هذه المعلومة
+
+457
+00:36:24,200 --> 00:36:27,500
+متناقدة مع المعلومة السابقة؟ آه، هي متناقدة
+
+458
+00:36:27,500 --> 00:36:31,920
+الصراحة، انه احنا .. طب stop يعني، احنا هلأ بدنا
+
+459
+00:36:31,920 --> 00:36:35,200
+نعرف ال plasma protein أو ال acute face protein هي
+
+460
+00:36:35,200 --> 00:36:39,680
+بتزود لزوجة، فبتقلل من معدل سقوط ال RBCs، يعني
+
+461
+00:36:39,680 --> 00:36:44,180
+بتقلل ال ESR و اللي هي بتعمل role of formation و
+
+462
+00:36:44,180 --> 00:36:49,520
+aggregation و بالتالي بتزود من تجمع ال RBCs فبتسرع
+
+463
+00:36:49,520 --> 00:36:54,340
+سقوتهاشوفوا معايا، هلأ ال acute face protein بتعمل
+
+464
+00:36:54,340 --> 00:36:59,180
+الشغلتين ولكن كفة .. الكفة تبع ال roll-off
+
+465
+00:36:59,180 --> 00:37:04,780
+formation و ال aggregation بترجح أكتر، تمام؟ بتكون
+
+466
+00:37:04,780 --> 00:37:10,320
+تأثيرها أكبر، ما طالما إن هي تأثيرها في اتجاه ال
+
+467
+00:37:10,320 --> 00:37:15,160
+roll-off and aggregation of RBCs أكبر، تمام؟
+
+468
+00:37:15,160 --> 00:37:22,040
+معناها الترسيب هيكون أسرعطبعا؟ طيب و .. لكن في
+
+469
+00:37:22,040 --> 00:37:26,560
+عندي بروتين اسمه ال albumin طبعا؟ هذا البروتين
+
+470
+00:37:26,560 --> 00:37:31,360
+تقريبا هو أعلى تركيز البروتين موجود في الدم يعني
+
+471
+00:37:31,360 --> 00:37:34,920
+موجود وبيتصنع بنسبة معينة هو أعلى بروتين موجود في
+
+472
+00:37:34,920 --> 00:37:41,420
+الدم طبعا؟هذا ال protein هو اللي بيعطي الدم قوامه،
+
+473
+00:37:41,420 --> 00:37:46,720
+ان هو يعني .. يعني أكتر لزوجة قليلا من الميه،
+
+474
+00:37:46,720 --> 00:37:50,420
+تمام؟ هو اللي .. هو اللي مخلي الدم مايصيرش ميه يا
+
+475
+00:37:50,420 --> 00:37:55,560
+بنات، هاي معناه بالعربي يعني هيك ال albumin، تمام؟
+
+476
+00:37:55,560 --> 00:38:01,100
+لو زاد ال albumin هذا نتيجة مرض معين، تمام؟يعني
+
+477
+00:38:01,100 --> 00:38:06,120
+زادت لزوجة الدم هنا شايفين ال album وين بلعب؟ بلعب
+
+478
+00:38:06,120 --> 00:38:10,540
+أكتر على كفة مين؟ ع كفة اللزوجة وليس ع كفة ال roll
+
+479
+00:38:10,540 --> 00:38:14,440
+of formation and aggregation طبعا؟ فإذا ال album
+
+480
+00:38:14,440 --> 00:38:20,660
+إذا زاد بزيد اللزوجة فبقلل الإياسار طبعا؟ إذا
+
+481
+00:38:20,660 --> 00:38:26,480
+خلينا نلخص هلأ ال protein بشكل عام زيادة تركيزها
+
+482
+00:38:26,480 --> 00:38:31,470
+في الدم طبعا؟ زيادة تركيزها في الدمممكن تعملي
+
+483
+00:38:31,470 --> 00:38:34,510
+زيادة في ال roll off formation و ال aggregation
+
+484
+00:38:34,510 --> 00:38:38,370
+فبالتالي بتسرع من سقوط ال RBCs فبالتالي بتسرع من
+
+485
+00:38:38,370 --> 00:38:45,470
+ال ESR تمام increased ESR او او ممكن لبروتينات
+
+486
+00:38:45,470 --> 00:38:49,770
+high تعمل increase في ال viscosity يعني زيادة في
+
+487
+00:38:49,770 --> 00:38:55,790
+اللزوجة لبلازمة فبالتالي بتعمل إعاقة لسقوط ال RBCs
+
+488
+00:38:55,790 --> 00:39:01,690
+فبتقلل ال ESR طيبالكافتين موجودات والاحتمالين
+
+489
+00:39:01,690 --> 00:39:07,050
+موجودين، طبعا، ولكن ال acute face protein بتشتغل
+
+490
+00:39:07,050 --> 00:39:11,770
+باتجاه ال roll of formation و ال aggregation أكثر،
+
+491
+00:39:11,770 --> 00:39:14,870
+طبعا، فبالتالي عشان هيك ال acute face protein
+
+492
+00:39:14,870 --> 00:39:21,290
+بتسرع ال ESR ولكن ال albumin زيادته هيشتغل باتجاه
+
+493
+00:39:21,290 --> 00:39:26,330
+زيادة ال viscosity أكثرتمام؟ فبالتالي زيادة ال ..
+
+494
+00:39:26,330 --> 00:39:31,610
+ال album يعني increased of viscosity يعني هيعمللي
+
+495
+00:39:31,610 --> 00:39:37,150
+inhibition للfall of RBCs يعني هيعطيني lower ESR
+
+496
+00:39:37,150 --> 00:39:44,230
+أو decreased ESRتمام؟ إذاً هذا ال .. ال .. يعني
+
+497
+00:39:44,230 --> 00:39:48,370
+دور ال plasma protein في ال ESR طيب في عندي أيضًا
+
+498
+00:39:48,370 --> 00:39:52,750
+mechanical and technical factors عوامل تقنية أو
+
+499
+00:39:52,750 --> 00:40:00,010
+ميكانيكية لحتى بتأثر على قيمة ال ESR في شغلنا
+
+500
+00:40:00,010 --> 00:40:06,370
+أثناء شغلنا و هاي العوامل بتأثر على قيمة ال ESR
+
+501
+00:40:06,960 --> 00:40:10,000
+يعني كيف انا اشتغلت العينة، كيف سحبتها، كيف
+
+502
+00:40:10,000 --> 00:40:12,480
+عملتلها mixing مع ال anticoagulant، نوع ال
+
+503
+00:40:12,480 --> 00:40:16,520
+anticoagulant اللي استخدمته، هل خليت ال .. ال tube
+
+504
+00:40:16,520 --> 00:40:20,260
+vertical او مش vertical، في درجة الحرارة، هذه كلها
+
+505
+00:40:20,260 --> 00:40:23,360
+عوامل mechanical، and technical عوامل تقنية
+
+506
+00:40:23,360 --> 00:40:29,410
+وميكانيكية بتؤثر على النتيجة طبعتيمثال عليها احنا
+
+507
+00:40:29,410 --> 00:40:34,210
+حكينا ان ال ESR should be exactly perpendicular
+
+508
+00:40:34,210 --> 00:40:39,510
+عمودية تصنع درجة تسعين درجة تمام؟ تصنع .. تصنع
+
+509
+00:40:39,510 --> 00:40:44,710
+زاوية تسعين درجة مع ال bench تمام؟ فبالتالي
+
+510
+00:40:44,710 --> 00:40:49,850
+الإيمالة ال tilt .. ال tilt يعني الإيمالة الإيمالة
+
+511
+00:40:49,850 --> 00:40:54,470
+في هذه ال tube تلاتين درجة هتعطيني error تلاتين في
+
+512
+00:40:54,470 --> 00:41:00,470
+الميةطبعا ال error 30% يعني 30% زيادة في قيمة ال
+
+513
+00:41:00,470 --> 00:41:06,690
+ESR لإن إيمالة ال tube بتزود ال ESR بتزود القيمة
+
+514
+00:41:06,690 --> 00:41:11,570
+ليش طيب بتزود القيمة؟ ال tube مائل يعني عندي سطح
+
+515
+00:41:11,570 --> 00:41:17,770
+مساحة سطح في .. لا ال roll off formation أكبر يعني
+
+516
+00:41:17,770 --> 00:41:24,480
+roll off formation أكتر يعني ESR أسرع و أعلىتمام؟
+
+517
+00:41:24,480 --> 00:41:27,720
+إذا هذا هيأثر على النتائج، أنا عندي إيه أسئلة
+
+518
+00:41:27,720 --> 00:41:29,860
+عالية، يعني هي ممكن أشك أن الشخص هذا عنده
+
+519
+00:41:29,860 --> 00:41:33,660
+infection, inflammation, tissue injury، فإذا .. و
+
+520
+00:41:33,660 --> 00:41:37,460
+النتيجة ممكن تكون falsely elevated، يعني هي مرتفعة
+
+521
+00:41:37,460 --> 00:41:40,820
+بالغلط، نتيجة a tilting، نتيجة إيه معناه في
+
+522
+00:41:40,820 --> 00:41:45,870
+الاتيوب؟طيب also the rack holding the tube الراك
+
+523
+00:41:45,870 --> 00:41:49,270
+اللي انا بدأ أحط عليه القنابيب تبعتي shouldn't be
+
+524
+00:41:49,270 --> 00:41:54,910
+subject to any movement or vibration مش لازم يتعرض
+
+525
+00:41:54,910 --> 00:42:01,260
+ليه اي اهتزازات او حركة هذا ال benchتمام؟ طيب ال
+
+526
+00:42:01,260 --> 00:42:03,760
+bench اللي أنا بدي أحط عليه الركات لازم يكون ثابت
+
+527
+00:42:03,760 --> 00:42:08,780
+لأ مافيش فيه عندي vibration اهتزازات أو حركة يعني
+
+528
+00:42:08,780 --> 00:42:11,760
+مثلا أنا بدي أحط ال rack تبع القنابيب تبعي بعيد عن
+
+529
+00:42:11,760 --> 00:42:15,820
+جهاز ال centrifuge بعيد عن التلاجة بعيد عن أي جهاز
+
+530
+00:42:15,820 --> 00:42:20,400
+مشغال و ممكن يعملي ذبذبات او اهتزازات او vibration
+
+531
+00:42:20,400 --> 00:42:24,080
+ل ال bench اللي محتوط عليه القنابيب تمام؟ حتى ما
+
+532
+00:42:24,080 --> 00:42:29,090
+تهتز القنابيب فبالتالي يتأثر ال sedimentationتمام،
+
+533
+00:42:29,090 --> 00:42:33,530
+هلأ minor everyday variation in room temperature
+
+534
+00:42:33,530 --> 00:42:40,420
+don't significantly affect the ACIتغير للقليل في
+
+535
+00:42:40,420 --> 00:42:45,800
+ال ESR .. في درجة الحرارة ما بيأثر على ال ESR
+
+536
+00:42:45,800 --> 00:42:48,980
+اليوم كانت درجة الحرارة تلاتين، امبارح تسعة و
+
+537
+00:42:48,980 --> 00:42:52,960
+عشرين، بكرا واحد و تلاتين لو أنا جبت عينة من شخص و
+
+538
+00:42:52,960 --> 00:42:56,380
+اشتغلتها على التلت أيام هذه في اختلاف درجات
+
+539
+00:42:56,380 --> 00:43:01,480
+الحرارة في ال minor variation هذا ممكن يعني نتيجة
+
+540
+00:43:01,480 --> 00:43:07,400
+ال ESR تكون نفسها أو فيها تغير بسيط جداما بيأثر
+
+541
+00:43:07,400 --> 00:43:10,960
+يعني don't significantly affect ESR يعني ما بيغير
+
+542
+00:43:10,960 --> 00:43:15,380
+كتير على نتيجة ال ESR لكن large changes in
+
+543
+00:43:15,380 --> 00:43:20,120
+temperature يعني مثلا تغير من عشرين لتلاتين من
+
+544
+00:43:20,120 --> 00:43:24,140
+خمستاشر لتلاتين يعني تغير كبير في درجة الحرارة
+
+545
+00:43:24,140 --> 00:43:28,140
+فهذا ممكن يأثر على نتيجة ال ESRالـ sedimentation
+
+546
+00:43:28,140 --> 00:43:31,960
+rate increases as temperature increases كل ما زاد
+
+547
+00:43:31,960 --> 00:43:37,120
+.. كل ما زادت درجة الحرارة بتزيد أو بيزيد قيمة ال
+
+548
+00:43:37,120 --> 00:43:43,120
+ESR the length and inner diameter of ESR tube also
+
+549
+00:43:43,120 --> 00:43:47,280
+affect the final test result كمان طول و القطر
+
+550
+00:43:47,280 --> 00:43:52,370
+الداخلي لل ESR tube بيأثر على ال test resultاحنا
+
+551
+00:43:52,370 --> 00:43:57,570
+حكينا أنبيب خاصة تتذكروا حكينا أنبيب خاصة لفحص ال
+
+552
+00:43:57,570 --> 00:44:01,770
+ESR كمان شوية راح نستعرضهم، راح نشوفهم، تمام؟ وهي
+
+553
+00:44:01,770 --> 00:44:07,050
+هم موجودين هم في الخلفية طيب فال ESR tube أنبيب ال
+
+554
+00:44:07,050 --> 00:44:14,850
+ESR كل ما كانت أضيق تمام؟ يعني أرفع ال .. ال .. ال
+
+555
+00:44:14,850 --> 00:44:17,750
+bore ال internal bore .. ال bore تبعها قليل أو
+
+556
+00:44:17,750 --> 00:44:23,180
+صغيرهتعطيني lower sedimentation rate، lower ESR
+
+557
+00:44:23,180 --> 00:44:29,780
+result، ليش؟ انبوب دقيق يعني أو .. او رفيع، ضيق،
+
+558
+00:44:29,780 --> 00:44:34,320
+معناه مافيش عندي حكينا مساحة سطح كافية ل ال role
+
+559
+00:44:34,320 --> 00:44:40,300
+of formation و ال RBCs aggregation فبالتالي ال ESR
+
+560
+00:44:40,300 --> 00:44:40,780
+هيقل
+
+561
+00:44:43,650 --> 00:44:47,990
+طيب إذا فائدة significance of the test أو
+
+562
+00:44:47,990 --> 00:44:52,790
+significance of the ESR فائدة الفحص تبعي حكينا إن
+
+563
+00:44:52,790 --> 00:44:58,330
+هو بيمثل ال ESR represent a non specific response
+
+564
+00:44:58,330 --> 00:45:01,910
+to tissue damage, inflammation, infection وجود
+
+565
+00:45:01,910 --> 00:45:08,190
+disease معين وما بيأثر على أو ما بيدل أو ما بيدلل
+
+566
+00:45:08,190 --> 00:45:11,710
+على ال severity طبعته but not its severity يعني
+
+567
+00:45:11,710 --> 00:45:16,900
+مثلابيدللي على وجود infection معينة زي التهابات
+
+568
+00:45:16,900 --> 00:45:22,040
+المفاصل أو inflammation معينة سوري زي التهابات
+
+569
+00:45:22,040 --> 00:45:24,920
+المفاصل infection معينة زي ال tuberculosis مرض
+
+570
+00:45:24,920 --> 00:45:30,940
+السلطن خلل في أعضاء معينة failure في أعضاء tissue
+
+571
+00:45:30,940 --> 00:45:36,910
+injury يعني هذه كلها ممكن يدللي عليهاولكن non
+
+572
+00:45:36,910 --> 00:45:40,510
+-specific حاكينا، طبعا يعني أنا ما بعرف وين ال
+
+573
+00:45:40,510 --> 00:45:43,990
+infection هاي وين ال inflammation هاي حادثة أو ال
+
+574
+00:45:43,990 --> 00:45:48,530
+tissue injury أنا بعرف إنه فيه، بعمل detection and
+
+575
+00:45:48,530 --> 00:45:51,590
+monitoring لأ ال tissue damage و ال inflammation و
+
+576
+00:45:51,590 --> 00:45:54,790
+ال infection ولكن بيكون non-specific يعني ما بعرف
+
+577
+00:45:54,790 --> 00:45:59,170
+وينها و ما بقدر أميز ال severity طبعتهاit's
+
+578
+00:45:59,170 --> 00:46:02,610
+primarily reflected changes in plasma protein طبعا
+
+579
+00:46:02,610 --> 00:46:06,350
+هو بيتأثر أكتر إشي في ال plasma protein اللي هي
+
+580
+00:46:06,350 --> 00:46:11,310
+عبارة اللي بتكون يعني اللي بتظهر أو ممكن تزيد أو
+
+581
+00:46:11,310 --> 00:46:15,190
+يتأثر تركيزها في حالات ال acute و ال chronic
+
+582
+00:46:15,190 --> 00:46:18,590
+infection في حالات ال tumor في حالات ال
+
+583
+00:46:18,590 --> 00:46:21,710
+degenerative disease يعني الأمراض الناتجة عن organ
+
+584
+00:46:21,710 --> 00:46:25,790
+failureطبعا هذه كلها بتأثر ال plasma بروتين
+
+585
+00:46:25,790 --> 00:46:29,250
+فبالتالي هيتأثر ال ESR و ممكن يكشفلي عنها نعمل ..
+
+586
+00:46:29,250 --> 00:46:33,350
+نعمل detection لقلها و أيضا حكينا بيعملي
+
+587
+00:46:33,350 --> 00:46:38,530
+monitoring طبعا يعني follow up ل ال progress of
+
+588
+00:46:38,530 --> 00:46:43,530
+certain disease يعني ممكن يعطيني .. يعني أشوف ال
+
+589
+00:46:43,530 --> 00:46:48,860
+..يعني أراقب وضع المريض بتحسن ولا ما بتحسن؟ بتحسن
+
+590
+00:46:48,860 --> 00:46:53,940
+ولا بزيد سوء؟ تمام؟ فهذا مثلا شخص مصاب بمرض السل
+
+591
+00:46:53,940 --> 00:46:57,080
+أو بمرض الـ Rheumatoid Arthritis التهاب المفاصل
+
+592
+00:46:58,070 --> 00:47:02,650
+طبعا فممكن يعني إذا أعطينا .. يعني ال ESR عملناله
+
+593
+00:47:02,650 --> 00:47:06,650
+يا كان عالي نتيجة inflammation عملناله specific
+
+594
+00:47:06,650 --> 00:47:11,510
+test فحوصات متخصصة أكثر فكشفت عن وجود إما
+
+595
+00:47:11,510 --> 00:47:16,110
+tuberculosis يعني سل عدوى يعني infection هذه طبعا
+
+596
+00:47:16,110 --> 00:47:20,350
+بكتيريا بتسبب مرض السل طبعا أو rheumatoid
+
+597
+00:47:20,350 --> 00:47:25,140
+arthritis التهابات المفاصلة ده مرض مناعي طبعاطيب
+
+598
+00:47:25,140 --> 00:47:29,380
+فاكتشفنا في الفحوصات المتخصة انه عنده اما
+
+599
+00:47:29,380 --> 00:47:33,320
+tuberculosis او rheumatoid arthritis الطبيب هيعطل
+
+600
+00:47:33,320 --> 00:47:39,100
+المريض علاجه ممكن لحتى نشوف استجابته او نشوف حالته
+
+601
+00:47:39,100 --> 00:47:44,960
+بتتحسن او بتسوق ممكن اشتغل ال ESR مرة تانية اشوفه
+
+602
+00:47:44,960 --> 00:47:51,500
+اقارنه بالنتجة السابقة زاد او نقصلكن يعني أنا ما
+
+603
+00:47:51,500 --> 00:47:57,320
+بحدد ال severity يعني مدى سوء الحالة من أول فحص
+
+604
+00:47:57,320 --> 00:48:03,400
+طبعا ممكن بعد العلاج أشوف الفحص التاني و أقارنه
+
+605
+00:48:03,400 --> 00:48:07,780
+بالأول عشان أحكم هو اتحسن ولا ما اتحسن لكن من فحص
+
+606
+00:48:07,780 --> 00:48:11,720
+واحد لأول مرة يعني ممكن يدللي إنه في infection أو
+
+607
+00:48:11,720 --> 00:48:17,360
+inflammationهو المريض وضعه تعبان على الآخر ولا في
+
+608
+00:48:17,360 --> 00:48:23,180
+بدايات المرض ممكن نلحج ولا لأ هذه أمور لا يمكن ان
+
+609
+00:48:23,180 --> 00:48:26,660
+احنا نعرفها من خلال ال ESR اللي هو فحص لحاله يعني
+
+610
+00:48:26,660 --> 00:48:32,500
+او انه نتيجة واحدة او حتى فحص لحاله بده other test
+
+611
+00:48:32,500 --> 00:48:35,100
+طيب
+
+612
+00:48:36,180 --> 00:48:41,380
+an elevated ESR may be found in زيادة ال ESR ممكن
+
+613
+00:48:41,380 --> 00:48:45,220
+تكون موجودة في حالات هلأ بتشوفوا الحالات هذه هتكون
+
+614
+00:48:45,220 --> 00:48:47,920
+إما عبارة عن inflammation أو infection أو tissue
+
+615
+00:48:47,920 --> 00:48:51,940
+injury خلينا نشوف acute and chronic infection
+
+616
+00:48:51,940 --> 00:48:55,120
+حالات ال acute و ال chronic infection في حالات ال
+
+617
+00:48:55,120 --> 00:48:58,460
+rheumatic fever اللي هو الحمه ال rheumatoid في
+
+618
+00:48:58,460 --> 00:49:03,680
+حالات ال rheumatoid arthritis التهيبات المفاصل
+
+619
+00:49:04,370 --> 00:49:08,530
+myocardial infection اللي هو عدوى او infection في
+
+620
+00:49:08,530 --> 00:49:13,330
+عضلة القلب nephrosis يعني infection في ال kidney
+
+621
+00:49:13,330 --> 00:49:18,190
+في ال nephrons acute hepatitis يعني التهابات في ال
+
+622
+00:49:18,190 --> 00:49:22,650
+liver menstruation ال period طب ما هي ال period
+
+623
+00:49:22,650 --> 00:49:26,410
+اصلا عبارة عن سقوط بطانة الرحم يعني tissue injury
+
+624
+00:49:26,410 --> 00:49:31,160
+صار طبعا فطبيعي يزيد ال ESRTuberculosis مراد السل،
+
+625
+00:49:31,160 --> 00:49:34,840
+infection high وأمراض الغضب الدراقية سواء كان
+
+626
+00:49:34,840 --> 00:49:39,180
+hypothyroidism يعني نقص في هرمونات الغضب الدراقية
+
+627
+00:49:39,180 --> 00:49:42,920
+أو hyperthyroidism يعني زيادة، hyp نقصان وhyper
+
+628
+00:49:42,920 --> 00:49:48,100
+زيادةالـ Pregnancy بعد الشهر التالت أيضًا بيزيد
+
+629
+00:49:48,100 --> 00:49:51,400
+فيها ال ESR بس مش بسبب وجود infection أو
+
+630
+00:49:51,400 --> 00:49:55,000
+inflammation أو tissue injury لأ انه في حق ال ..
+
+631
+00:49:55,000 --> 00:50:00,440
+ال pregnancy في الوضع الطبيعي بتزيد عندها بعد ش ..
+
+632
+00:50:00,440 --> 00:50:04,200
+ست الحامل بعد الشهر التالت بتزيد عندها نسبة ال
+
+633
+00:50:04,200 --> 00:50:06,900
+acute facial protein بيزيد عندها ال fibrinogen
+
+634
+00:50:06,900 --> 00:50:11,160
+طبعًا فطبيعي أنه ال ESR يزيد مش بسبب حالة مرضية
+
+635
+00:50:11,160 --> 00:50:15,500
+إما بحالة مؤقتة نتيجة وجود الحامل يعنيتمام؟ الشخص
+
+636
+00:50:15,500 --> 00:50:20,540
+اللي .. اللي عمره أكتر من ستين سنة تجاوز الستين
+
+637
+00:50:20,540 --> 00:50:25,200
+سنة ممكن ألاقي عنده slightly higher ESR يعني ESR
+
+638
+00:50:25,200 --> 00:50:30,380
+عالي نسبيا قليلا نتيجة إيش؟ أنه جسمه بشكل طبيعي
+
+639
+00:50:30,380 --> 00:50:35,180
+بلش ينتج album أقل من الطبيعي يعني أقل من ما هو
+
+640
+00:50:35,180 --> 00:50:41,230
+معتاد عليه في سن الشباب يعنيتمام؟ فإذا إجاني شخص
+
+641
+00:50:41,230 --> 00:50:46,610
+أكتر من ستين سنة وعملتله ESR ولاجئته عالي مش شرط
+
+642
+00:50:46,610 --> 00:50:49,670
+يكون عنده inflammation, infection or tissue injury
+
+643
+00:50:49,670 --> 00:50:55,110
+ممكن نتيجة السن أن هو جسمه بعد العمر هذا كله بطل
+
+644
+00:50:55,110 --> 00:50:58,930
+انه .. او يعني توقف عن الصناع المزيد او يعني كميات
+
+645
+00:50:58,930 --> 00:51:02,130
+كبيرة من ال album قلت نسبة ال album اللي بصنعها
+
+646
+00:51:02,130 --> 00:51:07,370
+يعني قلت لزوجة الدمإذا زاد ال ESR زاد الترسيب زاد
+
+647
+00:51:07,370 --> 00:51:14,150
+معدل سقوط ال RBCs طيب الأسباب التالية بت .. بت ..
+
+648
+00:51:14,150 --> 00:51:22,460
+يعني هي مسئولة عن decreased ESRdecreased ESR نقصان
+
+649
+00:51:22,460 --> 00:51:27,440
+ال ESR أو يعني قيمة قليلة لل ESR will be present
+
+650
+00:51:27,440 --> 00:51:31,720
+in هنشوفها وين في حالات ال polycythemia ذكرنا ليش؟
+
+651
+00:51:31,720 --> 00:51:35,820
+عندي RBCs كتيرة، بدها وقت لتسقط، role of formation
+
+652
+00:51:35,820 --> 00:51:40,480
+and aggregation قليل، viscosity عاليةفخصوصا لو
+
+653
+00:51:40,480 --> 00:51:44,040
+كانت حالتي ال polycythemia هذه سببها ورم فبالتالي
+
+654
+00:51:44,040 --> 00:51:49,200
+هيكون عندى ال ESR قليل ال RBCs بدها وقت أطول لتسقط
+
+655
+00:51:49,200 --> 00:51:54,940
+يعني المعدل سقوطها اشماله عالي معدل سقوطها قليل
+
+656
+00:51:54,940 --> 00:51:59,620
+سرعتها قليلة طبعا انا بحكي عن معدل سقوط يعني اشمال
+
+657
+00:51:59,620 --> 00:52:04,120
+.. يعني سرعة سرعتها قليلة فال ESR decreased في هذه
+
+658
+00:52:04,120 --> 00:52:09,160
+الحالة decreasedcongestive heart failure طبعا يعني
+
+659
+00:52:09,160 --> 00:52:14,940
+ف .. فشل في أي عضو من الأعضاء يعني أو في .. في
+
+660
+00:52:14,940 --> 00:52:20,860
+القلب تحديدا هذا هيعملي decreased ESR طيب ليش؟
+
+661
+00:52:20,860 --> 00:52:24,620
+congestive heart failure فشل في القلب يعني مافيش
+
+662
+00:52:24,620 --> 00:52:29,240
+ضخ دم كافيطبعا؟ يعني فيش oxygen delivery كافي،
+
+663
+00:52:29,240 --> 00:52:33,480
+طبعا؟ يعني في عندي hypoxia، نقص في ال oxygen، وين؟
+
+664
+00:52:33,480 --> 00:52:37,540
+في ال tissue و في ال organ، في جسم الإنسانطيب احنا
+
+665
+00:52:37,540 --> 00:52:42,060
+حكينا انه البون مارقش ماله بيشتغله مغمض عناق بس
+
+666
+00:52:42,060 --> 00:52:47,320
+بتحسس ال oxygen و اذا كان ناقص بيبدأ يصنع خلايا
+
+667
+00:52:47,320 --> 00:52:52,580
+بدون ما يفهم وين ال .. وين الخلل تمام؟ طيب بدون ما
+
+668
+00:52:52,580 --> 00:52:56,900
+يشوف انه المشكلة في القلب مش في التصنيع فبيبدأ
+
+669
+00:52:56,900 --> 00:53:02,420
+يصنع ايش RBC's كتيرةتطلع RBCs زيادة على ال
+
+670
+00:53:02,420 --> 00:53:05,420
+circulation إذا أصبحت الحالة بتشبه ال polycythemia
+
+671
+00:53:05,420 --> 00:53:10,500
+يعني زادت ال RBCs يعني بدها وقت أطول لإنه تترسم
+
+672
+00:53:10,500 --> 00:53:15,020
+يعني سرعتها هتكون بطيقة هذا معناه إنه بدها وقت
+
+673
+00:53:15,020 --> 00:53:20,560
+أطول لتترسم hypofibrogenemia يعني نقصان الفيبروجين
+
+674
+00:53:20,560 --> 00:53:26,470
+نقصان أحد ال acute face protein تمام؟نقصان الـ
+
+675
+00:53:26,470 --> 00:53:30,810
+fibrinogen اللي هو نقصان أحد ال .. ال acute face
+
+676
+00:53:30,810 --> 00:53:35,210
+protein بشكل طبيعي يعني الجسم ما بيصنع fibrinogen
+
+677
+00:53:35,210 --> 00:53:39,230
+أقل من الطبيعي فبالتالي برضه كمان هذا هيعمل لي
+
+678
+00:53:39,230 --> 00:53:44,310
+inhibition لل aggregation و ال raw formation of
+
+679
+00:53:44,310 --> 00:53:50,110
+RBCs فبالتالي هيكون في عندى ال ESR will decrease
+
+680
+00:53:50,750 --> 00:53:54,270
+كمان حالات الـ bukylocytosis و ال anisocytosis و
+
+681
+00:53:54,270 --> 00:53:58,730
+وجود .. وجود spherocytes و sickle cell في ال .. مع
+
+682
+00:53:58,730 --> 00:54:03,070
+خلايا أيضا يعني تباين في الحجم و الشكل هذا هيعمل
+
+683
+00:54:03,070 --> 00:54:05,330
+ليه inhibition لال roll off formation اذا
+
+684
+00:54:05,330 --> 00:54:12,270
+decreased ESR تمام؟طيب خلينا نشوف كيف احنا بنشتغل
+
+685
+00:54:12,270 --> 00:54:16,970
+فحص ال ESR طبعا في عندي نوعين او طريقتين انا بشتغل
+
+686
+00:54:16,970 --> 00:54:20,730
+فيهم حسب نوع ال tube اللي بشتغل فيها نوعين من ال
+
+687
+00:54:20,730 --> 00:54:25,750
+tubes وطريقتين طبعا ويستيرجرين method بستخدم فيها
+
+688
+00:54:25,750 --> 00:54:30,370
+أنابيب اسمها wystergren tube طبعا وفي عندي وينتروب
+
+689
+00:54:30,370 --> 00:54:34,950
+method بستخدم أنابيب اسمها وينتروب tube طبعا طيب
+
+690
+00:54:34,950 --> 00:54:41,630
+خلينا نشوف ال ال ..الوستيجرين method بيحكيلي انا
+
+691
+00:54:41,630 --> 00:54:46,110
+ممكن استخدم عينة عينة طبعا هو ال blood طبيعي اكيد
+
+692
+00:54:46,110 --> 00:54:50,950
+يعني الدم كامل بدون فصل انا باخده و طبعا اذا بدي
+
+693
+00:54:50,950 --> 00:54:54,670
+اخد مثلا اربع امقال لازم اضيفه على one امقال tube
+
+694
+00:54:54,670 --> 00:54:58,150
+فيها او على .. على tube فيها one امقال sodium
+
+695
+00:54:58,150 --> 00:55:03,460
+citrateطبعا؟ يعني النسبة أربعة لواحد ال dilution
+
+696
+00:55:03,460 --> 00:55:08,920
+هيكون كام؟ هيكون واحد لخمسة مين اللي مخفف؟ ال
+
+697
+00:55:08,920 --> 00:55:13,640
+citrate والله اللي مخفف لإنه اللي هو الكمية الأقل
+
+698
+00:55:13,640 --> 00:55:18,840
+صح؟ واحد لخمسة لما أحكي dilution واحد لخمسة طبعا؟
+
+699
+00:55:18,840 --> 00:55:23,800
+يعني انه .. إذا بدحكي خمسة لواحد بحكي عن ال blood
+
+700
+00:55:24,830 --> 00:55:28,970
+طبعا احنا اخدنا قوانين ال dilution فبإمكانكم انكم
+
+701
+00:55:28,970 --> 00:55:35,670
+تحسبوها و تعرفوا كم ال dilution طبعا اذا انا
+
+702
+00:55:35,670 --> 00:55:40,310
+استخدمت edita anticoagulated blood يعني ال blood
+
+703
+00:55:40,310 --> 00:55:43,490
+انا ماحطيته في تيوب فيه بعد ما جمعته ماحطيته في
+
+704
+00:55:43,490 --> 00:55:47,070
+تيوب فيها citrate انما حطيته في تيوب فيها edita
+
+705
+00:55:47,070 --> 00:55:53,470
+طبعاطبعا ف بيحكيلي انه هاي ال editor قبل ما انا
+
+706
+00:55:53,470 --> 00:55:59,690
+املأ .. املأ العينة بال Westigren tube لازم كل
+
+707
+00:55:59,690 --> 00:56:02,570
+اتنين امقال من ال blood ال anticoagulated بال
+
+708
+00:56:02,570 --> 00:56:09,530
+editor احطلهم نص امقال من محلول ملح فيه تركيز
+
+709
+00:56:09,530 --> 00:56:12,450
+point تمانية خمسة في المية sodium chloride اللي هو
+
+710
+00:56:12,450 --> 00:56:18,280
+ال salineتمام؟ أربع أمقال بدي أحطولهم واحد أمقال
+
+711
+00:56:18,280 --> 00:56:22,980
+sodium chloride solution اللي هو إيش بنسميه؟ بسميه
+
+712
+00:56:22,980 --> 00:56:26,600
+saline اللي هو المحلول الملح اللي إحنا ممكن نشتريه
+
+713
+00:56:26,600 --> 00:56:33,310
+من ال .. من السيدلياتهو المحلول الملحي أيضًا اللي
+
+714
+00:56:33,310 --> 00:56:39,270
+إحنا ممكن نحطه بالوريد للناس اللي بحتاجوا تخدية
+
+715
+00:56:39,270 --> 00:56:45,170
+وريدية فبالتالي هذا هو المحلول الملحي مصمم بتركيز
+
+716
+00:56:45,170 --> 00:56:50,410
+0.85% Sodium Chloride لحتى يحافظ على شكل الخلية
+
+717
+00:56:50,410 --> 00:56:55,630
+على الـ Osmotic Pressure تبع الخلية، تمام؟فيعني
+
+718
+00:56:55,630 --> 00:56:59,830
+بيستخدم لحتى أنا بضيفه على ال Edeta Tube طب ليش
+
+719
+00:56:59,830 --> 00:57:02,070
+بضيفه على ال Edeta لإنه ال Edeta مع ال Sodium
+
+720
+00:57:02,070 --> 00:57:05,410
+Chloride بيعطيني نفس ال Osmotic Pressure زي ما
+
+721
+00:57:05,410 --> 00:57:09,550
+أستخدم Sodium Citrate لحاله طبعا، إذا يا أما أربع
+
+722
+00:57:09,550 --> 00:57:14,190
+ML من ال Holy Blood بضيفهم على Tube فيها 1 ML من
+
+723
+00:57:14,190 --> 00:57:20,220
+Sodium Citrate أو أربع ML من ال Edeta بضيفلهمone
+
+724
+00:57:20,220 --> 00:57:26,100
+ml من ال .. ال sodium chloride solution تمام؟ و
+
+725
+00:57:26,100 --> 00:57:31,080
+بعد هيك ب .. يعني بعبي لل .. ال .. وستيجرين اتوم
+
+726
+00:57:31,080 --> 00:57:41,200
+تمام؟طيب ليش أنا ضفت على ال Edita .. يعني ليش ضفت
+
+727
+00:57:41,200 --> 00:57:44,320
+على ال Edita saline؟ أول إشي .. أول سبب علشان
+
+728
+00:57:44,320 --> 00:57:49,160
+أحافظ على ال osmolality تمام؟ و تانيش إشي يا بنات
+
+729
+00:57:49,160 --> 00:57:53,900
+عشان .. عامل ال dilution هلأ ال sodium citrate ..
+
+730
+00:57:53,900 --> 00:57:56,900
+ال sodium citrate لما أنا أحط one ml volume هذا
+
+731
+00:57:56,900 --> 00:58:02,480
+تمام؟ هذا حجمالستريد سائل و أنا حطيت على الأربعة
+
+732
+00:58:02,480 --> 00:58:09,260
+أمقال ل blood one أمقال من هذا الستريد طيب لكن ال
+
+733
+00:58:09,260 --> 00:58:15,120
+Edita بتكون يا بنات powder وجزء لا يكاد يذكر يكون
+
+734
+00:58:15,120 --> 00:58:19,060
+ممسوح في .. في .. في تيوب ال Edita فلما أجي أنا
+
+735
+00:58:19,060 --> 00:58:24,610
+أضيف أربعة أمقال على تيوب ال Edita ما بتغيروشأو
+
+736
+00:58:24,610 --> 00:58:27,790
+بيزيدوا بنسبة ضئيلة جدا، يعني بيضلوا أربعة زي ما
+
+737
+00:58:27,790 --> 00:58:30,990
+هما، هان أربعة أنا ضيفت عليها one أم ال من ال
+
+738
+00:58:30,990 --> 00:58:33,330
+secret يعني صاروا ال volume خمسة ال total volume
+
+739
+00:58:33,330 --> 00:58:37,190
+أما هنا ال Edita مع ال blood لما أنا أضيف ال blood
+
+740
+00:58:37,190 --> 00:58:41,030
+على ال tube فيها Edita، بيضال أربعة زي ما هو، لإن
+
+741
+00:58:41,030 --> 00:58:46,050
+ال Edita بودر وي بتكون يدوب يعني على الجدار ال
+
+742
+00:58:46,050 --> 00:58:51,330
+tube، كمية لات كانت تذكرفانا لازم اضيف ال one ml
+
+743
+00:58:51,330 --> 00:58:57,290
+solution من ال saline solution علشان احافظ على نفس
+
+744
+00:58:57,290 --> 00:59:01,150
+النسب نفس ال dilution نفس ال proportion انا اخدت
+
+745
+00:59:01,150 --> 00:59:06,130
+تمام؟ اتمنى انها تكون واضحة و بعد ما انا املأ
+
+746
+00:59:06,130 --> 00:59:09,750
+الwistigrine pipe او الwistigrine tube بخليها
+
+747
+00:59:09,750 --> 00:59:16,410
+allow it stand بخليها انها تكونو يعني بال vertical
+
+748
+00:59:16,410 --> 00:59:21,610
+position لمدة one hour و بعد هيك أنا بقيس المسافة
+
+749
+00:59:21,610 --> 00:59:24,430
+اللي تقطعها ال RBC بال millimeter per one hour
+
+750
+00:59:24,430 --> 00:59:30,050
+المعدد الطبيعي للنساء من 0 ل 15 عند الرجال من 0 ل
+
+751
+00:59:30,050 --> 00:59:33,810
+10 أو عند الأطفال أيضا من 0 ل 10 millimeter per
+
+752
+00:59:33,810 --> 00:59:39,870
+hourطبعا عند النساء أكتر لإنه النساء يعني نسبة ال
+
+753
+00:59:39,870 --> 00:59:43,870
+plasma protein عندهم مختلفة تمام؟ بعدين النساء
+
+754
+00:59:43,870 --> 00:59:48,230
+بتكون يعني إما طالعة من .. من period أو فايتة على
+
+755
+00:59:48,230 --> 00:59:51,730
+period أو يعني .. يعني فال plasma protein و ال
+
+756
+00:59:51,730 --> 00:59:55,350
+tissue injury و الحلجات هادى يعني بتكون .. بتأثر و
+
+757
+00:59:55,350 --> 00:59:59,270
+.. فبالتالي هي نسبتها بتكون أعلى بشكل طبيعي يعني
+
+758
+00:59:59,270 --> 01:00:00,090
+هاد بتكون أعلى
+
+759
+01:00:03,160 --> 01:00:10,460
+طمام؟ إذا هذا هو ال normal ranges طيب
+
+760
+01:00:10,460 --> 01:00:14,340
+إيش محتاجة؟ محتاجة للتجربة تبعتي عينة ال blood
+
+761
+01:00:14,340 --> 01:00:17,720
+اللي أنا بدي أسحبها و أحطها في ال .. في ال .. إما
+
+762
+01:00:17,720 --> 01:00:21,940
+في سترات أو في إيدي تتيوب لو حطيتها في سترات خلاص
+
+763
+01:00:21,940 --> 01:00:26,390
+يعني هي 4 ml blood على 1 ml sodium citrateو إذا
+
+764
+01:00:26,390 --> 01:00:29,110
+حطيتها في إيدي تتيوب لازم أضيف one and a half من
+
+765
+01:00:29,110 --> 01:00:32,590
+ال saline عشان بالنهاية أنا بدي نفس النسبة و نفس
+
+766
+01:00:32,590 --> 01:00:35,810
+ال dilution و نفس ال proportion و نفس ال is
+
+767
+01:00:35,810 --> 01:00:39,550
+molality تمام؟ بعدين بملأ ال westigran tube زي ما
+
+768
+01:00:39,550 --> 01:00:42,830
+أنتوا شايفين ال westigran tube بهذا الشكل و لازم
+
+769
+01:00:42,830 --> 01:00:47,110
+يكون إلها أبعاد طبعا إحنا حكينا انبوب خاصة يعني
+
+770
+01:00:47,110 --> 01:00:50,430
+ماقاش معنا specific tube يعني إنها لازم تكون
+
+771
+01:00:50,430 --> 01:00:56,960
+أبعادها خاصة أو standardالطول تبعها ال external
+
+772
+01:00:56,960 --> 01:01:00,940
+diameter القطر الخارجي القطر الداخلي عليها تدريج
+
+773
+01:01:00,940 --> 01:01:07,690
+معين هذا كله لازم يكون يعني ضمن معاييرو أيضا بحتاج
+
+774
+01:01:07,690 --> 01:01:12,790
+ال rack لهاي ال .. القنابيب هذا اللي لونه أزرق هو
+
+775
+01:01:12,790 --> 01:01:17,430
+اللي بسميه rack، تمام؟ و طبعا كل ال rackات لازم
+
+776
+01:01:17,430 --> 01:01:21,590
+تكون فيها حاجة زي ال bulb بتأكدلي أن هذا ال rack
+
+777
+01:01:21,590 --> 01:01:26,850
+يعني جالس بشكل طبيعي أو بشكل يعني كويس على ال
+
+778
+01:01:26,850 --> 01:01:31,730
+bench فهو عمودي، مية بالمية عمودي، تمام؟
+
+779
+01:01:34,970 --> 01:01:39,670
+يعني كيف هيكون فيها bulb خليني بس أذكرلكم مثال
+
+780
+01:01:39,670 --> 01:01:44,270
+شوفتوا عامل البناء اللي بيكون بيبني حيطان أو إشي
+
+781
+01:01:44,270 --> 01:01:47,970
+بيكون معاه زي مصطرة حديدية بيسميها ميزان وهي
+
+782
+01:01:47,970 --> 01:01:52,110
+المصطرة الحديدة في نصها في نصها في انبوبة زجاجية
+
+783
+01:01:52,110 --> 01:01:56,270
+فيها سائل أصفرطبعا هذه العامل البناء بيبني الحائط
+
+784
+01:01:56,270 --> 01:02:00,130
+بعد هيك او يعني لما بيجي بده يبني بيحط ال .. مصدر
+
+785
+01:02:00,130 --> 01:02:05,930
+الحائط طبعته و بيشوف مدى استيواق او اعتدال الحائط
+
+786
+01:02:06,660 --> 01:02:11,260
+لو كان في عندي خلل في مستوى الماء يعني في إيمالة
+
+787
+01:02:11,260 --> 01:02:15,660
+فيه معناه هو الله الاشي مش عمودي أو مش مستوي و هنا
+
+788
+01:02:15,660 --> 01:02:18,160
+نفس الفكرة يعني لازم يكون في عندي bulb لازم يكون
+
+789
+01:02:18,160 --> 01:02:22,020
+في عندي علامة من خلالها أعرف إن هذا ال .. الراك
+
+790
+01:02:22,020 --> 01:02:27,010
+جالس بالشكل ال vertical أو لاو أيضا كمان لازم يكون
+
+791
+01:02:27,010 --> 01:02:33,650
+في عندي timer لحتى يعني أضبطه على one hour و
+
+792
+01:02:33,650 --> 01:02:37,890
+يحسبلي ساعة و ي run بعد نهاية الساعة عشان أجي أقرأ
+
+793
+01:02:37,890 --> 01:02:45,400
+النتائجطيب إذا أنا إيش محتاجة؟ محتاجة إما عينة
+
+794
+01:02:45,400 --> 01:02:52,380
+blood أربع أمقال في سترية one ml أو أحط أربع أمقال
+
+795
+01:02:52,380 --> 01:02:59,580
+من ال Edita و أحط عليهم one ml من ال .. ال saline
+
+796
+01:02:59,580 --> 01:03:03,760
+أو كل اتنين أمقال أضيفلهم نص من ال sodium chloride
+
+797
+01:03:03,760 --> 01:03:08,220
+solution اللي هو ال saline، تمام؟هي في عندكم point
+
+798
+01:03:08,220 --> 01:03:12,420
+تمانية خمسة في المية wet per volume عندكم بمحاضرة
+
+799
+01:03:12,420 --> 01:03:16,100
+للمناقش ان شاء الله هسألكم كيف حضرناها هذه عشان
+
+800
+01:03:16,100 --> 01:03:19,120
+تعرفولي ال wet per volume احنا كيف حضرنا محلول ال
+
+801
+01:03:19,120 --> 01:03:19,500
+saline
+
+802
+01:03:22,320 --> 01:03:25,720
+طبعا التجربة مش رح أشرحها لإنها شبه صارت واضحة و
+
+803
+01:03:25,720 --> 01:03:30,940
+لإنه إحنا راح نشتغل بالمعامل بطريقة مختلفة طبعا لا
+
+804
+01:03:30,940 --> 01:03:35,040
+هي Winthrop ولا Westgren طريقة مختلفة فخلينا ..
+
+805
+01:03:35,040 --> 01:03:37,520
+يعني أنتوا هيكصرتوا أصلا already فاهمين يعني إيش
+
+806
+01:03:37,520 --> 01:03:44,910
+التجربة أو كيف بتنشغل ولكن يعني خلونا ..نختصر
+
+807
+01:03:44,910 --> 01:03:52,430
+موضوع ال procedure Wintrop and Landsberg method او
+
+808
+01:03:52,430 --> 01:03:57,580
+اختصارا بنحكي عنها Wintrop methodتمام؟ الفكرة هنا
+
+809
+01:03:57,580 --> 01:04:02,000
+أنه أنا ممكن أستخدم Edita Anti-coagulated blood
+
+810
+01:04:02,000 --> 01:04:05,940
+تمام؟ و على طول بس هك أحط و أعبيه في tube اسمها
+
+811
+01:04:05,940 --> 01:04:11,620
+windrop tube و أجلسها لمدة one hour شك على الوضع
+
+812
+01:04:11,620 --> 01:04:15,900
+ال vertical و بعد هك أقرأ النتائج فعندي أنه النساء
+
+813
+01:04:15,900 --> 01:04:23,160
+المعدل الطبيعي من 0 ل 20 و الرجال
+
+814
+01:04:23,160 --> 01:04:31,090
+من 0 ل 9طبعا فهذه هي ال .. ال normal ranges ل ال
+
+815
+01:04:31,090 --> 01:04:33,930
+windrop tube إيش محتاجة؟ محتاجة أتيوبات windrop
+
+816
+01:04:33,930 --> 01:04:37,890
+محتاجة ال rack تبعهم محتاجة .. يعني ممكن أحتاج
+
+817
+01:04:37,890 --> 01:04:44,710
+capillary pipe أو يعني محتاجة a sticker يسكر طرف
+
+818
+01:04:44,710 --> 01:04:49,030
+الإنبوبة من جهة عشان أثبتها على ال rack بعد هيك و
+
+819
+01:04:49,030 --> 01:04:54,190
+محتاجة whole blood طبعاone ml using edita
+
+820
+01:04:54,190 --> 01:05:00,730
+anticoagulant نفس الفكرة عينة plot مجموعة في edita
+
+821
+01:05:00,730 --> 01:05:05,790
+tube بعبي منها ال capillary pipet أو يعني بعبي
+
+822
+01:05:05,790 --> 01:05:07,890
+منها باستخدام ال capillary pipet اللي هي ال wind
+
+823
+01:05:07,890 --> 01:05:13,650
+turbine tube و بحط بسكرها بانطرفها و بعد هيك بحطها
+
+824
+01:05:13,650 --> 01:05:17,990
+على ال rack بال vertical position لمدة ساعة و بقرأ
+
+825
+01:05:17,990 --> 01:05:22,360
+هاي هنا أهل ال procedure تبعتيطبعا خلينا بس نستعرض
+
+826
+01:05:22,360 --> 01:05:29,860
+كيف شكل ال .. ال .. ال wind drop tube او sorry هاي
+
+827
+01:05:29,860 --> 01:05:34,840
+ال westigran هاي westigran tube مع ال .. ال sodium
+
+828
+01:05:34,840 --> 01:05:37,780
+citrate tube اللي بيكون فيها one ml sodium citrate
+
+829
+01:05:37,780 --> 01:05:42,560
+و هذه هي ال ESR tube مدرجة طبعا انا بفوتها و بيكون
+
+830
+01:05:42,560 --> 01:05:47,620
+نهاية التدريج عند zero طبعا فالمنطقة ال zero بتكون
+
+831
+01:05:47,620 --> 01:05:49,040
+في الأعلى طبعا
+
+832
+01:05:55,410 --> 01:06:00,950
+هي .. ها دي .. بحكي عنها winter بتيوب، هي أسمك
+
+833
+01:06:00,950 --> 01:06:07,230
+شوية، تمام؟
+
+834
+01:06:07,230 --> 01:06:16,750
+تمام
+
+835
+01:06:20,360 --> 01:06:27,220
+طيب نيجي لمناقشة بعض النقاط المتعلقة بالفحص ال ESR
+
+836
+01:06:27,220 --> 01:06:34,440
+أول إشي أثناء ترسيب خلايا ال RBCs تمر عملية
+
+837
+01:06:34,440 --> 01:06:39,090
+الترسيب بثلاث مراحلالمرحلة الأولى اللى هى خلال أول
+
+838
+01:06:39,090 --> 01:06:42,650
+عشر دقائق فى فحص الترسيب خلال أول عشر دقائق فى
+
+839
+01:06:42,650 --> 01:06:46,710
+الساعة بسميها ال lag phase و فى هذا .. فى هذا
+
+840
+01:06:46,710 --> 01:06:50,190
+المرحلة بيكون الخلايا بس عم تعمل roll off
+
+841
+01:06:50,190 --> 01:06:53,870
+formation تمام؟ ال sedimentation بيكون قليل slight
+
+842
+01:06:53,870 --> 01:06:58,960
+قليلطبعا طيب بعد في الاربع .. في الاربعين دقيقة
+
+843
+01:06:58,960 --> 01:07:04,340
+اللتي تليها بيبدأ او بيكون في طور او مرحلة اسمها
+
+844
+01:07:04,340 --> 01:07:07,280
+ال decantation phase او ال sedimentation phase
+
+845
+01:07:07,280 --> 01:07:10,640
+اللي بيكون فيها عملية ال sedimentation اشمالها
+
+846
+01:07:10,640 --> 01:07:15,940
+سريعة rapid and constant سريعة ومعدل ثابت للترسيب
+
+847
+01:07:16,460 --> 01:07:21,360
+طيب في آخر عشر دقائق بيحدث ال stationary phase ان
+
+848
+01:07:21,360 --> 01:07:27,280
+هو يعني بس مجرد تراكم و تفنيش لالترسيب يعني انه هو
+
+849
+01:07:27,280 --> 01:07:31,020
+finalization بس فقط لغير انه بيكون في عندي
+
+850
+01:07:31,020 --> 01:07:36,320
+accumulation of RBCs صار في تراكم لل RBCs في قاع
+
+851
+01:07:36,320 --> 01:07:39,500
+ال tube و صار في عندي ان ال sedimentation rate
+
+852
+01:07:39,500 --> 01:07:46,230
+بيكون slow و ..طبعا كل ما كان ال tube أطول كل ما
+
+853
+01:07:46,230 --> 01:07:49,350
+كان طول في هذا ال face بيكون .. يعني بياخد وقت
+
+854
+01:07:49,350 --> 01:07:53,890
+أطول هذا ال face طبعا هذا إذا ال stationary face
+
+855
+01:07:53,890 --> 01:08:00,010
+طيب إذا بتسأله عنهم إيش اسم ال face و ماذا يحدث
+
+856
+01:08:00,010 --> 01:08:05,450
+فيه طبعا ممكن أسألك عنه في .. انه في mentionفي ..
+
+857
+01:08:05,450 --> 01:08:12,230
+يعني مقال قصير أو ممكن في ال .. في ال in the blank
+
+858
+01:08:12,230 --> 01:08:17,550
+في المصطلح أو حتى في ال mcq في ال .. الاختيار من
+
+859
+01:08:17,550 --> 01:08:21,970
+المتعدد بتسألي عنهم هدول المعلومات فمهم تحفظيهم كل
+
+860
+01:08:21,970 --> 01:08:29,390
+واحد إيش بيصير فيه مدته واسم للمرحلة طيباحنا حكينا
+
+861
+01:08:29,390 --> 01:08:34,090
+إنه لتيوب بيكون مدرج بحيث إنه أعلى تدريج بيكون
+
+862
+01:08:34,090 --> 01:08:38,490
+لتحت و ال zero فوق، تمام؟ و أنا بملأ هاي لتيوب
+
+863
+01:08:38,490 --> 01:08:44,380
+فلازم أملأها لعندي ال zero، تمام؟عشان لما أجى أقرأ
+
+864
+01:08:44,380 --> 01:08:49,220
+أقرأ سقوط ال RPCs أقرأها من ال zero لوين لمتى يكون
+
+865
+01:08:49,220 --> 01:08:53,580
+الطرقة سهلة عندي طيب لو نفرض أنه أنا كمية ال blood
+
+866
+01:08:53,580 --> 01:08:56,480
+كانت ماكانتش كافية أن أنا أملأ كل tube بحيث أنه
+
+867
+01:08:56,480 --> 01:09:00,260
+يصل نهاية ال blood عند ال zero mark وصل عند ال
+
+868
+01:09:00,260 --> 01:09:05,340
+واحد mark او ال اتنين ليه قبل ال zero طبعا فمعناها
+
+869
+01:09:05,340 --> 01:09:10,860
+انا عندي volume أقل من ال volume اللي لازم اشتغل
+
+870
+01:09:10,860 --> 01:09:16,290
+عليه بواحد او اتنين مليطبعا لنفرض أنه كانت النتيجة
+
+871
+01:09:16,290 --> 01:09:23,250
+نهائية 18 18 إيش؟ هي يعني 18 كانت ترسيب مع 2 فاقد
+
+872
+01:09:23,250 --> 01:09:28,630
+2 نقص في ال volume طبعا إذا لأ ساعتها عشان أجيب
+
+873
+01:09:28,630 --> 01:09:33,490
+النتيجة الدقيقة بسجل النتيجة 18 نقص 2 ويساوي 16 mm
+
+874
+01:09:33,490 --> 01:09:38,230
+per hourطيب لو كانت ال .. ال .. عين .. يعني مستوى
+
+875
+01:09:38,230 --> 01:09:41,510
+ال blood وصل أقل من .. يعني لتحت الصفر بواحد،
+
+876
+01:09:41,510 --> 01:09:44,950
+باتنين، بتلاتة، باربعة، بشتغل بهذه الطريقة لكن
+
+877
+01:09:44,950 --> 01:09:49,350
+خمسة فأكثر أو أقل من الخمسة حتى يعني أنه تحت
+
+878
+01:09:49,350 --> 01:09:54,030
+الخمسة يعني في فقدان أكبر من خمسة millimeter هذا
+
+879
+01:09:54,030 --> 01:09:59,350
+معناها فبالتالي هيكون النتيجة not valid يعني لا
+
+880
+01:09:59,350 --> 01:10:03,640
+تُأخد بيهاnot .. not considered لا تعتبر يعني ما
+
+881
+01:10:03,640 --> 01:10:09,840
+تأخدش بعين الاعتبار لازم أعيد التجربة طيب فإذا ال
+
+882
+01:10:09,840 --> 01:10:15,010
+..النتيجة إذا كانت يعني إنه إذا كان الحجم تم
+
+883
+01:10:15,010 --> 01:10:18,850
+تعبئته لتحت الخمسة يعني في فاقد أكتر من خمسة
+
+884
+01:10:18,850 --> 01:10:22,490
+فبالتالي لازم أنا أعيد النتيجة if the upper level
+
+885
+01:10:22,490 --> 01:10:26,010
+of blood is below zero due to leakage of blood
+
+886
+01:10:26,010 --> 01:10:29,930
+from the bottom of tube فبالتالي أيضا النتيجة
+
+887
+01:10:29,930 --> 01:10:34,890
+invalid لو كان في عندي نزل مستوى blood عن ال zero
+
+888
+01:10:34,890 --> 01:10:38,750
+نتيجة تسريب احنا حكينا إنه في ال leakage هيكون فيه
+
+889
+01:10:38,750 --> 01:10:44,970
+فقدان لل RBCs أكترمن ال plasma فبالتالي هذا هيأثر
+
+890
+01:10:44,970 --> 01:10:49,350
+على النتيجة ويخليها لا يأخذ بيها، تمام؟ فلازم أعيد
+
+891
+01:10:49,350 --> 01:10:52,970
+الفحص All the sedimentation racks should be
+
+892
+01:10:52,970 --> 01:10:56,170
+equipped with leveling screw and super spirit
+
+893
+01:10:56,170 --> 01:10:59,480
+levelلازم تكون هذه ال .. ال racks اللي أنا
+
+894
+01:10:59,480 --> 01:11:04,700
+بستخدمها في عندها أمان أو ضابط أو إشي يعني يكون
+
+895
+01:11:04,700 --> 01:11:08,700
+دايما أكدلي أنه أنا المستوى اللي بشتغل عليه مستوى
+
+896
+01:11:08,700 --> 01:11:15,440
+أفقي و ال cube عمودي 100% تمام؟ يصنع زاوية 90 درجة
+
+897
+01:11:15,440 --> 01:11:22,240
+mechanical source of error خلل أو مصدر خلل نتيجة
+
+898
+01:11:22,240 --> 01:11:29,090
+mechanical errorطيب إذا زادت تركيز ال editة، طبعا؟
+
+899
+01:11:29,090 --> 01:11:33,730
+إذا زادت تركيز ال editة، طبعا؟ فيعني .. يعني ..
+
+900
+01:11:33,730 --> 01:11:36,690
+إيش يعني زاد تركيز ال editة؟ مش إحنا حكينا
+
+901
+01:11:36,690 --> 01:11:40,270
+القنابيب معلمة، يعني أنا لازم أعبيها لعند علامة
+
+902
+01:11:40,270 --> 01:11:45,360
+معينة، طبعا عبيت القنابيب أقل من العلامةيعني blood
+
+903
+01:11:45,360 --> 01:11:49,720
+نسبته أقل من ما ينبغي بالنسبة لل Edeta يعني ال
+
+904
+01:11:49,720 --> 01:11:53,880
+Edeta زادت، تمام؟ فإذا زادت ال Edeta حكينا إنها
+
+905
+01:11:53,880 --> 01:11:57,620
+بتقلل ال ESR وهي المعلومة مرت علينا قبل هيك طب
+
+906
+01:11:57,620 --> 01:12:01,260
+ليش؟ لإنها بتعمل inhibition ل ال roll-off
+
+907
+01:12:01,260 --> 01:12:06,900
+formation ال ESR بيقل نتيجة inhibition لل roll-off
+
+908
+01:12:06,900 --> 01:12:10,440
+formation لإن ال Edeta إذا زادت هتعمل inhibition
+
+909
+01:12:10,440 --> 01:12:16,000
+لل roll-off formationطيب، أيضًا ال anticoagulant
+
+910
+01:12:16,000 --> 01:12:20,680
+of sodium أجزالات or potassium أجزالات الهيبرين
+
+911
+01:12:20,680 --> 01:12:26,240
+بيعملولي ال RBCs بيسببوا الشرنكش لل RBCs، يعني
+
+912
+01:12:26,240 --> 01:12:31,600
+اضمحلال أو ضمور لل RBCs أو يعني انت هتكرمش ال RBCs
+
+913
+01:12:32,010 --> 01:12:36,390
+فبالتالي حجم ال RBCs ال sediment تبعها هيقل
+
+914
+01:12:36,390 --> 01:12:42,130
+البلازمة هتزيد على حساب ال RBCs يعني إيش؟ أنا بقيس
+
+915
+01:12:42,130 --> 01:12:45,310
+المسافة اللي سقطتها، طب هي المسافة اللي سقطتها؟
+
+916
+01:12:45,310 --> 01:12:48,090
+يعني سقطت مسافة و اضمحلت نتيجة وجود ال
+
+917
+01:12:48,090 --> 01:12:53,610
+anticoagulant، ضمرتطبعا فهذا هيزيد من قيمة EAS R
+
+918
+01:12:53,610 --> 01:12:58,590
+falsely elevated خلل هذا هو مش عالي لإنه في خلل
+
+919
+01:12:58,590 --> 01:13:04,950
+عند المريض لأ هو عالي نتيجة خلل في سحب العين او في
+
+920
+01:13:04,950 --> 01:13:10,590
+اختيار النوع المناسب لل anticoagulantإذا if the
+
+921
+01:13:10,590 --> 01:13:14,590
+ESR stand for more than 60 minutes the result will
+
+922
+01:13:14,590 --> 01:13:17,850
+be falsely elevated وبرضه كمان لو أنا نسيت ل tube
+
+923
+01:13:17,850 --> 01:13:23,350
+أكتر من ساعة ممكن أيضا النتيجة ESR تكون زيادة
+
+924
+01:13:23,350 --> 01:13:28,390
+تمام؟ و هذا طبعا إذا كان أيضا ال .. ال .. الوقت
+
+925
+01:13:28,390 --> 01:13:33,210
+أقل من ساعة هذا برضه كمان ممكن يعملي low value ف
+
+926
+01:13:33,210 --> 01:13:38,670
+.. و invalid يعني ما ..لا يُأخذ بها طبعا mark the
+
+927
+01:13:38,670 --> 01:13:41,890
+increase or decrease in room temperature lead to
+
+928
+01:13:41,890 --> 01:13:45,170
+increase or decrease ESR result احنا حكينا زيادة
+
+929
+01:13:45,170 --> 01:13:49,270
+ال room temperature كل ما زادت درجة الحرارة هتزيد
+
+930
+01:13:49,270 --> 01:13:54,360
+قيمة ال ESRtilting of the ESR tube increasing the
+
+931
+01:13:54,360 --> 01:13:59,020
+ESR إي مالة الأنابيد يعني إنها ما تكون في ال
+
+932
+01:13:59,020 --> 01:14:02,980
+vertical position تكون ميلة هتزود ال ESR وحكينا
+
+933
+01:14:02,980 --> 01:14:07,920
+ليش لإنه بيصير عندي مساحة سطح بسبب الميلان هذا
+
+934
+01:14:07,920 --> 01:14:11,920
+بيصير عندي مساحة سطح لل roll off formation أكبر
+
+935
+01:14:11,920 --> 01:14:17,820
+طبعا؟ طيبBubble in blood cause invalid result
+
+936
+01:14:17,820 --> 01:14:22,840
+فقعات من الهواء في الأنابيب
+
+937
+01:14:23,760 --> 01:14:27,340
+يعني برضه كمان هتأثر على النتيجة هتعطيني invalid
+
+938
+01:14:27,340 --> 01:14:32,120
+result و .. لإنه ايضا انا يعني بقيس مسافة فلو
+
+939
+01:14:32,120 --> 01:14:35,680
+المسافة هذه مشغولة يعني في فنوسها bubble يعني فإذا
+
+940
+01:14:35,680 --> 01:14:40,320
+القراعة تبعتي مش هتكون دقيقة fibrin clot present
+
+941
+01:14:40,320 --> 01:14:43,500
+in the blood invalidate result برضه كمان أنه يصير
+
+942
+01:14:43,500 --> 01:14:47,390
+في عندى clotting في عينة الدمأيضا هيأثر على
+
+943
+01:14:47,390 --> 01:14:51,230
+النتيجة، هيخلي نتيجة لا أخذ بها، وهذا ال fiber
+
+944
+01:14:51,230 --> 01:14:54,290
+nickelot ممكن تظهر نتيجة ان انا ماعمل mixing
+
+945
+01:14:54,290 --> 01:14:58,260
+للعينة مع ال anticoagulant بشكل كافيفصار في
+
+946
+01:14:58,260 --> 01:15:02,640
+clotting يعني aggregation لل RBCs بدون صار في
+
+947
+01:15:02,640 --> 01:15:07,020
+aggregation يعني هترسب بسرعة و هده يعني نتيجة خلال
+
+948
+01:15:07,020 --> 01:15:12,200
+error the ESR should be set within two hours of
+
+949
+01:15:12,200 --> 01:15:16,520
+blood collection أنا لازم أشتغل ال ESR خلال ساعتين
+
+950
+01:15:16,520 --> 01:15:21,180
+من جمع العينة إذا أنا بشتغل يعني إذا اعتمدت ال
+
+951
+01:15:21,180 --> 01:15:26,120
+EDTA anticoagulant هممكن يعطيني ست ساعاتيعني وقت
+
+952
+01:15:26,120 --> 01:15:32,380
+يعني اللي بنحكي عنه يعني الوقت الفاصل ما بين جمع
+
+953
+01:15:32,380 --> 01:15:37,440
+العينة ويعني أنه أنا أجي أشتغلها ممكن معايا لحد ست
+
+954
+01:15:37,440 --> 01:15:40,300
+ساعات إذا كانت العينة refrigerated باستخدام ال
+
+955
+01:15:40,300 --> 01:15:45,480
+editor تمام؟ باستخدام set rate؟ لأ ساعتين تمام،
+
+956
+01:15:45,480 --> 01:15:47,660
+هلأ ال windrop ESR
+
+957
+01:15:50,850 --> 01:15:55,510
+طبعا انبوبة windrup more sensitive لو ال ESR له
+
+958
+01:15:55,510 --> 01:16:03,970
+طبعا يعني لو ال ESR قليل اي .. يعني اي تغير في
+
+959
+01:16:03,970 --> 01:16:09,190
+قيمته هي .. هي .. يعني هي ال tube هتتمكن من انها
+
+960
+01:16:09,190 --> 01:16:14,010
+تكشف ليه عنه طبعا اذا sensitive اي تغير في ال ESR
+
+961
+01:16:14,010 --> 01:16:18,070
+اللي قيمته قليلة ال windrup method يعني افضل له
+
+962
+01:16:19,650 --> 01:16:25,710
+ممكن تعمله detection أما الوستيجرين method تمام هي
+
+963
+01:16:25,710 --> 01:16:30,630
+more accurate لو كان ال ESR عالي تمام؟ و
+
+964
+01:16:30,630 --> 01:16:33,510
+الوستيجرين method has been chosen as the standard
+
+965
+01:16:33,510 --> 01:16:36,110
+يعني هي ال standard method و المتمدة أكتر
+
+966
+01:16:36,110 --> 01:16:40,330
+الوستيجرين methodWhen reading the ESR result on
+
+967
+01:16:40,330 --> 01:16:43,930
+blood with an extremely high YBCs or platelet
+
+968
+01:16:43,930 --> 01:16:48,050
+count, the buffy coat should be excluded نفس فكرة
+
+969
+01:16:48,050 --> 01:16:51,450
+الهيموتقرات لما أنا كنت بقيس الهيموتقرات كنت بقيس
+
+970
+01:16:51,450 --> 01:16:56,510
+نسبة ال RBCs فقط بدون ال buffy coatطبعا و ايضا لما
+
+971
+01:16:56,510 --> 01:17:00,290
+بدي اقيس ال ESR الترسيب هشوف مسافة اللي قطعتها
+
+972
+01:17:00,290 --> 01:17:03,550
+يعني مسافة البلازمة او الحجم .. المسافة اللي
+
+973
+01:17:03,550 --> 01:17:11,010
+احتلتها البلازمة طبعا و .. يعني هذا الاشي بدون ما
+
+974
+01:17:11,010 --> 01:17:13,790
+انا اقرأ ال buffy quote يعني should be excluded
+
+975
+01:17:13,790 --> 01:17:17,290
+يعني ال buffy quote يعني بيكون خارج القراءة تبعتي
+
+976
+01:17:17,290 --> 01:17:21,450
+if there is poor separation of the RBCs and the
+
+977
+01:17:21,450 --> 01:17:27,660
+plasma layerthis may be due to increased number of
+
+978
+01:17:27,660 --> 01:17:31,300
+reticulocyte و بسمي stratified sedimentation هلأ
+
+979
+01:17:31,300 --> 01:17:37,200
+إيش معنى هذه الجملة؟ هلأ في نوع من الخلائق أو يعني
+
+980
+01:17:37,200 --> 01:17:42,720
+مرحلة من مراحل نضوج ال RBCs بسميها reticulocyte
+
+981
+01:17:42,720 --> 01:17:46,900
+مرحلة ال reticulocyte هي مرحلة ما قبل خروجها
+
+982
+01:17:46,900 --> 01:17:51,990
+مباشرة على ال circulationطبعا هي نفس الشكل يعني
+
+983
+01:17:51,990 --> 01:17:55,490
+الخلية ال RBCs احنا نعرف انها أثناء نضوجها بتمر
+
+984
+01:17:55,490 --> 01:17:59,850
+بمراحل في ال bone marrow أخر مرحلة في ال bone
+
+985
+01:17:59,850 --> 01:18:02,550
+marrow بتكون ال reticulocyte و بعدين بتطلع على ال
+
+986
+01:18:02,550 --> 01:18:07,050
+circulation طبعا ال reticulocyte بتشبه الخلية اللي
+
+987
+01:18:07,050 --> 01:18:09,810
+موجودة في ال circulation الخلية اللي احنا بنعرفها
+
+988
+01:18:09,810 --> 01:18:17,040
+اللي هي شكلهاتمام؟ ولكن بيكون فيها يعني زي شبكة
+
+989
+01:18:17,040 --> 01:18:21,620
+RNA لسه ما اتخلصت من ال RNA تبعها بتطلع .. يعني
+
+990
+01:18:21,620 --> 01:18:24,700
+نسبة ال reticulocyte بشكل طبيعي بالجسم الإنسان
+
+991
+01:18:24,700 --> 01:18:29,280
+واحد في المية واحد لا اتنين في المية .. هك يعني و
+
+992
+01:18:29,280 --> 01:18:33,980
+بتطلع هاي ال reticulocyte على الدم بتاخد لها يوم و
+
+993
+01:18:33,980 --> 01:18:38,440
+بتندج يعني بت .. خلاص بتختفي ال RNA و بتصير ال
+
+994
+01:18:38,440 --> 01:18:44,330
+RBCS طبيعيةطيب increase number of reticulocyte متى
+
+995
+01:18:44,330 --> 01:18:49,710
+بيزيد هذا العدد من ال reticulocyte؟ متى بتزيد نسبة
+
+996
+01:18:49,710 --> 01:18:53,610
+ال reticulocyte في ال circulation و بيزيد نسبتها و
+
+997
+01:18:53,610 --> 01:18:57,930
+بيزيد إنتاجها على ال .. يعني او خروجها من البون
+
+998
+01:18:57,930 --> 01:19:05,250
+مرة؟نتيجة ال blood loss يعني فقدان دم أنيميا تكسر
+
+999
+01:19:05,250 --> 01:19:10,750
+خلايا ال RBCs أي أمرض ممكن ي .. ي .. ينشأ عنه
+
+1000
+01:19:10,750 --> 01:19:15,170
+فقدان RBCs ممكن ال bone marrow يروح يصير ضغط عليه
+
+1001
+01:19:15,170 --> 01:19:19,090
+أنه والله بدنا تصنيع أكتر يعني طلع أكتر RBCs احنا
+
+1002
+01:19:19,090 --> 01:19:22,990
+عم نفقد RBCs تمام؟ فبالتالي ممكن يطلع ال
+
+1003
+01:19:22,990 --> 01:19:25,410
+reticulosa فيها مش ناضجة بس يلا ده بروح حالكوا
+
+1004
+01:19:25,410 --> 01:19:29,920
+فيها شوية يعني هنا بدها يوموا تندجطبعا؟ طيب هذه ال
+
+1005
+01:19:29,920 --> 01:19:34,420
+reticulocyte مع خلايا الدم الطبيعية اللي موجودة في
+
+1006
+01:19:34,420 --> 01:19:39,100
+الدم يعني مختلفة في اختلاف ما بينهم في الكثافة في
+
+1007
+01:19:39,100 --> 01:19:44,840
+الحجم في ال .. في الوزم يعني هي نفس الشكل تقريبا
+
+1008
+01:19:44,840 --> 01:19:48,980
+بس يعني حجمها أكبر شوية كثافتها مختلفة فقل وزمها
+
+1009
+01:19:48,980 --> 01:19:54,260
+مختلفطبعا فلما تيجي ترسب مابترسبش كلها بطبقة واحدة
+
+1010
+01:19:54,260 --> 01:19:58,900
+الخلايا بيصير في عندي ترسيب مخطط stratified
+
+1011
+01:19:58,900 --> 01:20:03,280
+sedimentation يعني طبقة خلايا بلازمة طبقة خلايا
+
+1012
+01:20:03,280 --> 01:20:10,100
+بلازمة و هكذا طبعا فبيصير في عندي يعني زي البوب
+
+1013
+01:20:10,100 --> 01:20:15,500
+بيصير مخططة RBCs أحمر أصفر أحمر أصفر زي هيك طبعا
+
+1014
+01:20:15,500 --> 01:20:19,650
+RBCs بلازمة RBCs بلازمة و هكذانتيجة وجود
+
+1015
+01:20:19,650 --> 01:20:24,710
+reticulocytosis او زيادة في نسبة ال reticulocyte
+
+1016
+01:20:24,710 --> 01:20:28,370
+the internal bore and the length of a graduated
+
+1017
+01:20:28,370 --> 01:20:30,670
+scale on the western pipe are critical
+
+1018
+01:20:30,670 --> 01:20:34,990
+measurements حكينا قطرها و طولها للأنبوب لازم يكون
+
+1019
+01:20:34,990 --> 01:20:39,510
+standard انا بستخدم قنابيب خاصة لان القنابيب اذا
+
+1020
+01:20:39,510 --> 01:20:43,940
+كانت مثلا اذا زادرفعها يعني مافيش مساحة سطح كافية
+
+1021
+01:20:43,940 --> 01:20:48,700
+لل .. لل .. لل ESR ممكن يقل لإن مافيش مساحة سطح
+
+1022
+01:20:48,700 --> 01:20:51,860
+كافية لل roll of formation بيقل ال ESR و نبوب
+
+1023
+01:20:51,860 --> 01:20:55,640
+سميكة يعني مساحة سطح أكبر لل roll of formation
+
+1024
+01:20:55,640 --> 01:21:00,680
+هيزيد ال ESR تمام؟ فبالتالي أنه لازم تكون الأنابيب
+
+1025
+01:21:00,680 --> 01:21:03,580
+standard لما أجي أشتغل فيها عشان أطلع نتائج
+
+1026
+01:21:03,580 --> 01:21:04,640
+accurate and precise
+
+1027
+01:21:09,520 --> 01:21:16,080
+disposable ESR مرت معانا هذه الجملة أو هذا المصطلح
+
+1028
+01:21:16,080 --> 01:21:21,580
+disposable طبعا و معنا استخدام لمرة واحدة فقط و
+
+1029
+01:21:21,580 --> 01:21:25,160
+بعدين هيطلف disposable يعني one use لما أحكيلكم
+
+1030
+01:21:25,160 --> 01:21:29,560
+هذه الأداة disposable إيش يعني؟ يعني استخدميها و
+
+1031
+01:21:29,560 --> 01:21:34,020
+بعدين قتلي فيها ارميها طبعا تخلصي منها هي ما بنرجع
+
+1032
+01:21:34,020 --> 01:21:37,990
+نستخدمها مرة تانيةهلأ و في ون تروب و في وستي جريني
+
+1033
+01:21:37,990 --> 01:21:42,230
+تيوب زجاجية و بنغسلها و بنعقمها بعد كل استخدام و
+
+1034
+01:21:42,230 --> 01:21:45,690
+بنرجع نستخدمها مرة تانية لكن في أنابيب بلاستيكية
+
+1035
+01:21:45,690 --> 01:21:49,330
+زي اللي موجودة في الخلفية هاي فاستخدام لمرة واحدة
+
+1036
+01:21:49,330 --> 01:21:53,490
+فقط و يتم التخلص منها هذه بسميها disposable ESR
+
+1037
+01:21:53,490 --> 01:21:59,610
+فعندي commercial kit كتات تجارية صارت تجيني متاحة
+
+1038
+01:21:59,610 --> 01:22:04,550
+صارت now available لال disposable ESRطبعا ال civil
+
+1039
+01:22:04,550 --> 01:22:07,830
+kit include safety cap for the babies that allow
+
+1040
+01:22:07,830 --> 01:22:10,970
+the blood to precisely fill to the zero بيكون طبعا
+
+1041
+01:22:10,970 --> 01:22:16,490
+في زي صمام موجود في خلايا .. في هذه النبوبة و طبعا
+
+1042
+01:22:16,490 --> 01:22:20,250
+أثناء دخول أنا .. يعني أنا هاي ال tube إلها صمام و
+
+1043
+01:22:20,250 --> 01:22:23,070
+بدخلها داخل عينة الدم تبعتي، انبوبة الدم اللي أنا
+
+1044
+01:22:23,070 --> 01:22:28,040
+شعبتهتمام؟ و هذا الصمام بيسمح انه الارق ..
+
+1045
+01:22:28,040 --> 01:22:32,580
+الانبوبة تفوت و تمتلق بال blood لعند ايش؟ لعند
+
+1046
+01:22:32,580 --> 01:22:36,480
+علامة ال zero هاي شايفين لو انتوا جيتوا هنا نكبر
+
+1047
+01:22:36,480 --> 01:22:42,820
+هاي الصورة و نطلع في الخلفية هاي الانبوب zeroخمسة،
+
+1048
+01:22:42,820 --> 01:22:47,080
+عشرة، تمام؟ إحنا حكينا إن الأنابيب لازم أعبيها لل
+
+1049
+01:22:47,080 --> 01:22:51,940
+zero blood اللي هي زي هيك لعند ال zero أعبيها ما
+
+1050
+01:22:51,940 --> 01:22:56,900
+تكون .. لو كانت وصلت للواحد، للاتنين، للتلقال ت ..
+
+1051
+01:22:56,900 --> 01:23:00,740
+ها دي يعني ها ممكن أنا أحل المشكلة by subtraction
+
+1052
+01:23:00,740 --> 01:23:03,900
+يعني أطرح في النهاية القيمة اللي تطلع معايا من
+
+1053
+01:23:03,900 --> 01:23:10,310
+كمية الفاقد أو النقصفي ال volume طبعا لو كانت يعني
+
+1054
+01:23:10,310 --> 01:23:14,070
+تحت الخمسة يعني في عندي فاقد انا اكتر من خمسة
+
+1055
+01:23:14,070 --> 01:23:19,770
+تمام؟ فبالتالي هذه invalid result طيب احنا بنحكي
+
+1056
+01:23:19,770 --> 01:23:24,440
+انه في ال disposable اي أسئار tube فيها صمامبيسمح
+
+1057
+01:23:24,440 --> 01:23:29,160
+مرور ل tube داخل عينة الدم ويرتفع فيها الدم الى
+
+1058
+01:23:29,160 --> 01:23:33,520
+نسبة ال .. ال zero level تمام بشكل دقيق و هذا
+
+1059
+01:23:33,520 --> 01:23:37,360
+بيخلي ان ال by بتتصير a closed system تصير نظام
+
+1060
+01:23:37,360 --> 01:23:42,080
+مغلق .. مغلق يعني هذا طبعا ال .. ال cab او ال ..
+
+1061
+01:23:44,050 --> 01:23:49,570
+الصمام بي .. بيضل ثابت على ال tube على عينة الدم و
+
+1062
+01:23:49,570 --> 01:23:53,090
+بعدين هك أنا بحطهم في الركات بهذا الشكل و الرك
+
+1063
+01:23:53,090 --> 01:23:57,470
+بيكون مدرج الخلفية بحسب ساعة على ال stop watch و
+
+1064
+01:23:57,470 --> 01:24:02,730
+بعدين هك باجي بقرأ النتيجة تبعتي، تمام؟ فهي .. هذا
+
+1065
+01:24:02,730 --> 01:24:08,190
+معناه ال ESR أو فكرة ال ESR ان شاء الله بس نرجع
+
+1066
+01:24:08,190 --> 01:24:14,930
+للمعامل بنطبقها عملي و حكينا انههتشتغله بطريقة
+
+1067
+01:24:14,930 --> 01:24:19,250
+مختلفة يعني هعطيكم قنابيب زجاجية بتعبوها blood at
+
+1068
+01:24:19,250 --> 01:24:23,730
+level معين و بعد هيك نجلسها على الراك و نحسب طبعا
+
+1069
+01:24:23,730 --> 01:24:26,990
+ال kit اللي تحكيلي او تعليمات ال kit او الشركة
+
+1070
+01:24:26,990 --> 01:24:30,290
+المصنعة لل kit اللي تحكيلي احصي بنص ساعة فقط و ليس
+
+1071
+01:24:30,290 --> 01:24:34,890
+ساعة و بعد هيك القنابيب disposable يعني اتخلصي
+
+1072
+01:24:34,890 --> 01:24:39,430
+منهم تمام؟ هذه اللي احنا راح نشتغلها في المختبرات
+
+1073
+01:24:39,430 --> 01:24:47,900
+تجربة نص ساعةو ان شاء الله إذا الله رد إلنا عودة و
+
+1074
+01:24:47,900 --> 01:24:53,240
+بنشتغل العملية هيك بنكون وصلنا لنهاية محاضرتنا
+
+1075
+01:24:53,240 --> 01:24:58,300
+لليوم و لكن في عندي بعض الصور لسه بدي أعرضها معاكم
+
+1076
+01:24:58,300 --> 01:25:01,360
+هي هنا في عندي هاي الصورة
+
+1077
+01:25:04,130 --> 01:25:09,610
+شايفين؟ هي هنا عندي fibrinogen البروتين تمام و زي
+
+1078
+01:25:09,610 --> 01:25:13,230
+مثلا عندي antibodies globulin بروتين هذه
+
+1079
+01:25:13,230 --> 01:25:19,230
+البروتينات بتروح إيش بتعمل؟ بتحيط ال RBC بتقلل من
+
+1080
+01:25:19,230 --> 01:25:23,790
+الشحنة السالبة، بتقلل من ال zeta potential تمام؟
+
+1081
+01:25:23,790 --> 01:25:28,360
+هي added positive chargeبتضيف شحنة موجة بقى
+
+1082
+01:25:28,360 --> 01:25:32,620
+neutralize negative charge بتعادل الشحنة السالي
+
+1083
+01:25:32,620 --> 01:25:37,600
+بقى ال zeta potential بيقل reduce zeta potential
+
+1084
+01:25:37,600 --> 01:25:41,500
+يعني هتقلل ال repulsion forces و تقلل المسافة ما
+
+1085
+01:25:41,500 --> 01:25:46,310
+بين ال RBCs مما يؤدي إلى ايش؟ تجمع ال RBCsstack of
+
+1086
+01:25:46,310 --> 01:25:50,810
+RBCs يعني rollout formation وهذا هيقدّي إلى إيش؟
+
+1087
+01:25:50,810 --> 01:25:55,410
+سرعة سقوط ال RBCs إيش يعني rollout؟ يعني إنها تكون
+
+1088
+01:25:55,410 --> 01:26:00,150
+ملتصقة مع بعض هالبعض like stack coins تمام؟
+
+1089
+01:26:00,150 --> 01:26:04,650
+ومذكورة في أحد ال slides
+
+1090
+01:26:22,920 --> 01:26:26,620
+مدكور في أحد ال slides في ال .. ال comments تحت
+
+1091
+01:26:26,620 --> 01:26:30,340
+إيش يعني rollout formation؟ rollout formation يعني
+
+1092
+01:26:30,340 --> 01:26:34,480
+RBCs aggregate like stack coins هورجيكم إياها و
+
+1093
+01:26:34,480 --> 01:26:36,100
+أختم المحاضرة ان شاء الله
+
+1094
+01:27:45,840 --> 01:27:56,900
+rolox معناها RBCs aggregate like
+
+1095
+01:27:56,900 --> 01:28:00,620
+stack
+
+1096
+01:28:00,620 --> 01:28:03,020
+coins
+
+1097
+01:28:04,420 --> 01:28:08,640
+زي القطع المعدنية أو اللي هي أو العملة الملتصقة مع
+
+1098
+01:28:08,640 --> 01:28:12,520
+بعضها البعض اللي هي يعني قائص أفطة الشواكر اللي
+
+1099
+01:28:12,520 --> 01:28:16,880
+أنا دربتلكم مثال عليها تمام؟ و هيك أيضا بت .. و
+
+1100
+01:28:16,880 --> 01:28:20,460
+هيك تعريفها في الكتب يعني RBCs aggregate like
+
+1101
+01:28:20,460 --> 01:28:24,000
+stack of coins يعني زي قطع معدنية منزلقة على بعضها
+
+1102
+01:28:24,000 --> 01:28:25,780
+البعض أو ملتصقة ببعضها البعض
+
+1103
+01:28:28,590 --> 01:28:32,730
+وصلنا لنهاية محاضرتنا لليوم، يعطيكم ألف عافية
+
+1104
+01:28:32,730 --> 01:28:34,010
+وموفقين بإبن الله
+
diff --git a/PL9fwy3NUQKwY9jl8xw-bWkVZkWcGGyspd/Bu-y4fC_shI_raw.json b/PL9fwy3NUQKwY9jl8xw-bWkVZkWcGGyspd/Bu-y4fC_shI_raw.json
new file mode 100644
index 0000000000000000000000000000000000000000..777b23a71cc006909589b4471e10528fb68f89ae
--- /dev/null
+++ b/PL9fwy3NUQKwY9jl8xw-bWkVZkWcGGyspd/Bu-y4fC_shI_raw.json
@@ -0,0 +1 @@
+{"segments": [{"id": 1, "seek": 1780, "start": 2.83, "end": 17.81, "text": "السلام عليكم ورحمة الله وبركاته طالبات العزيزات اليوم ان شاء الله راح نشرح محاضرة erythrocyte sedimentation rate erythrocyte معناها red blood cell خلايا الدم الحمراء sedimentation", "tokens": [6027, 3794, 37440, 25894, 24793, 4032, 2288, 35571, 3660, 21984, 4032, 26890, 4117, 9307, 3224, 23032, 6027, 3555, 9307, 18863, 11622, 1829, 11622, 9307, 45595, 20498, 16472, 13412, 16606, 21984, 12602, 39319, 8717, 46309, 5016, 3714, 5016, 46958, 25720, 1189, 88, 392, 340, 1344, 975, 32362, 399, 3314, 1189, 88, 392, 340, 1344, 975, 20449, 8315, 11296, 2182, 3390, 2815, 16490, 15040, 25528, 32748, 2304, 21542, 29973, 16606, 32362, 399], "avg_logprob": -0.11036531606190642, "compression_ratio": 1.5197740112994351, "no_speech_prob": 1.138448715209961e-05, "words": [{"start": 2.83, "end": 3.31, "word": "السلام", "probability": 0.8406575520833334}, {"start": 3.31, "end": 3.83, "word": " عليكم", "probability": 0.9560546875}, 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concentration of RBCs قليل في حالة الانيميا، تركيز الخلايا، عدد الخلايا قليل في ال plasma فال aggregation و ال role of formation هيزيد، تمام؟", "tokens": [2288, 5016, 7251, 28543, 11703, 1829, 30731, 36145, 2423, 12564, 49, 7251, 11622, 25708, 12399, 11933, 15730, 2423, 9856, 295, 497, 7869, 82, 12174, 20292, 1211, 8978, 11331, 6027, 3660, 2423, 7649, 32640, 25528, 12399, 6055, 31747, 1829, 11622, 33962, 15040, 25528, 12399, 6225, 3215, 3215, 33962, 15040, 25528, 12174, 20292, 1211, 8978, 2423, 22564, 6156, 6027, 16743, 399, 4032, 2423, 3090, 295, 11723, 39896, 11622, 25708, 12399, 46811, 10943, 22807], "avg_logprob": -0.1694878472222222, "compression_ratio": 1.5227272727272727, "no_speech_prob": 1.3887882232666016e-05, "words": [{"start": 1790.5, "end": 1791.28, "word": "رح", "probability": 0.715576171875}, {"start": 1791.28, "end": 1791.66, "word": " يقدّي", "probability": 0.75897216796875}, {"start": 1791.66, "end": 1791.84, "word": " إلى", "probability": 0.6455078125}, {"start": 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polycythemia هذه سببها ورم فبالتالي هيكون عندى ال ESR قليل ال RBCs بدها وقت أطول لتسقط يعني المعدل سقوطها اشماله عالي معدل سقوطها قليل سرعتها قليلة طبعا انا بحكي عن معدل سقوط يعني اشمال .. يعني سرعة سرعتها قليلة فال ESR decreased في هذه الحالة decreased", "tokens": [5172, 9778, 9381, 2407, 9381, 995, 45164, 25961, 2655, 11331, 6027, 31371, 2423, 6754, 1344, 392, 14058, 29538, 8608, 3555, 3555, 11296, 4032, 2288, 2304, 6156, 3555, 6027, 2655, 6027, 1829, 39896, 30544, 43242, 7578, 2423, 12564, 49, 12174, 20292, 1211, 2423, 497, 7869, 82, 47525, 11296, 4032, 38149, 5551, 9566, 12610, 5296, 2655, 3794, 47432, 37495, 22653, 9673, 22488, 1211, 8608, 4587, 2407, 9566, 11296, 1975, 8592, 2304, 6027, 3224, 6225, 6027, 1829, 3714, 22488, 1211, 8608, 4587, 2407, 9566, 11296, 12174, 20292, 1211, 8608, 2288, 34268, 11296, 12174, 20292, 37977, 23032, 3555, 3615, 995, 1975, 8315, 4724, 5016, 4117, 1829, 18871, 20449, 3215, 1211, 8608, 4587, 2407, 9566, 37495, 22653, 1975, 8592, 2304, 6027, 4386, 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ال westigran tube بهذا الشكل و لازم يكون إلها أبعاد طبعا إحنا حكينا انبوب خاصة يعني ماقاش معنا specific tube يعني إنها لازم تكون أبعادها خاصة أو standard", "tokens": [2407, 11933, 15730, 11331, 9566, 36081, 11296, 8978, 11933, 1829, 16254, 6055, 31371, 37746, 5296, 31377, 2304, 5551, 11242, 33911, 472, 293, 257, 1922, 9154, 2423, 1845, 533, 6225, 8592, 7649, 20666, 1863, 11296, 10632, 41850, 4724, 16254, 8717, 36178, 28239, 35457, 3660, 4032, 8717, 36178, 2423, 11504, 1448, 4032, 8717, 36178, 2423, 16068, 4032, 8717, 36178, 2423, 307, 8015, 1860, 46811, 10943, 22807, 39182, 9957, 4724, 42213, 10721, 2423, 7009, 328, 4257, 9917, 30767, 1829, 19446, 14739, 2655, 14407, 13412, 995, 33911, 9957, 2423, 7009, 328, 4257, 9917, 39627, 15730, 25124, 28820, 4032, 5296, 31377, 2304, 7251, 30544, 11933, 1211, 11296, 5551, 3555, 3615, 18513, 23032, 3555, 3615, 995, 11933, 5016, 8315, 11331, 4117, 1829, 8315, 16472, 3555, 37746, 16490, 33546, 3660, 37495, 22653, 19446, 4587, 33599, 20449, 8315, 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"probability": 0.9395751953125}, {"start": 3644.79, "end": 3645.41, "word": " انبوب", "probability": 0.861328125}, {"start": 3645.41, "end": 3646.27, "word": " خاصة", "probability": 0.9913736979166666}, {"start": 3646.27, "end": 3647.11, "word": " يعني", "probability": 0.767822265625}, {"start": 3647.11, "end": 3647.43, "word": " ماقاش", "probability": 0.6981608072916666}, {"start": 3647.43, "end": 3647.73, "word": " معنا", "probability": 0.835205078125}, {"start": 3647.73, "end": 3648.17, "word": " specific", "probability": 0.955078125}, {"start": 3648.17, "end": 3648.71, "word": " tube", "probability": 0.9306640625}, {"start": 3648.71, "end": 3649.37, "word": " يعني", "probability": 0.972412109375}, {"start": 3649.37, "end": 3649.75, "word": " إنها", "probability": 0.7447509765625}, {"start": 3649.75, "end": 3650.11, "word": " لازم", "probability": 0.9944661458333334}, {"start": 3650.11, "end": 3650.43, "word": " تكون", "probability": 0.989501953125}, {"start": 3650.43, "end": 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"end": 3875.93, "word": " محتاجة", "probability": 0.9239501953125}, {"start": 3875.93, "end": 3876.13, "word": " ..", "probability": 0.3349609375}, {"start": 3876.13, "end": 3876.95, "word": " يعني", "probability": 0.85693359375}, {"start": 3876.95, "end": 3877.35, "word": " ممكن", "probability": 0.993896484375}, {"start": 3877.35, "end": 3877.89, "word": " أحتاج", "probability": 0.9002278645833334}, {"start": 3877.89, "end": 3878.33, "word": " capillary", "probability": 0.681884765625}, {"start": 3878.33, "end": 3878.67, "word": " pipe", "probability": 0.70458984375}, {"start": 3878.67, "end": 3880.09, "word": " أو", "probability": 0.404052734375}, {"start": 3880.09, "end": 3881.29, "word": " يعني", "probability": 0.9091796875}, {"start": 3881.29, "end": 3883.23, "word": " محتاجة", "probability": 0.931396484375}, {"start": 3883.23, "end": 3883.39, "word": " a", "probability": 0.345947265625}, {"start": 3883.39, "end": 3883.73, "word": " sticker", "probability": 0.97119140625}, {"start": 3883.73, "end": 3884.27, "word": " يسكر", "probability": 0.94287109375}, {"start": 3884.27, "end": 3884.71, "word": " طرف", "probability": 0.98681640625}, {"start": 3884.71, "end": 3885.49, "word": " الإنبوبة", "probability": 0.8434326171875}, {"start": 3885.49, "end": 3885.65, "word": " من", "probability": 0.99560546875}, {"start": 3885.65, "end": 3886.07, "word": " جهة", "probability": 0.9114583333333334}, {"start": 3886.07, "end": 3886.53, "word": " عشان", "probability": 0.9822591145833334}, {"start": 3886.53, "end": 3887.15, "word": " أثبتها", "probability": 0.74873046875}, {"start": 3887.15, "end": 3887.35, "word": " على", "probability": 0.8818359375}, {"start": 3887.35, "end": 3887.49, "word": " ال", "probability": 0.96044921875}, {"start": 3887.49, "end": 3887.69, "word": " rack", "probability": 0.93994140625}, {"start": 3887.69, "end": 3887.99, "word": " بعد", "probability": 0.9775390625}, {"start": 3887.99, "end": 3888.55, "word": " هيك", "probability": 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"text": "طبعا فهذا هيزيد من قيمة EAS R falsely elevated خلل هذا هو مش عالي لإنه في خلل عند المريض لأ هو عالي نتيجة خلل في سحب العين او في اختيار النوع المناسب لل anticoagulant", "tokens": [9566, 3555, 3615, 995, 6156, 3224, 15730, 39896, 11622, 25708, 9154, 12174, 32640, 3660, 462, 3160, 497, 16720, 736, 23457, 16490, 1211, 1211, 23758, 31439, 37893, 6225, 6027, 1829, 5296, 28814, 1863, 3224, 8978, 16490, 1211, 1211, 43242, 9673, 16572, 11242, 5296, 10721, 31439, 6225, 6027, 1829, 8717, 31371, 7435, 3660, 16490, 1211, 1211, 8978, 8608, 5016, 3555, 18863, 9957, 1975, 2407, 8978, 1975, 9778, 31371, 9640, 28239, 45367, 9673, 8315, 35457, 24976, 2511, 2789, 559, 425, 394], "avg_logprob": -0.1908623391314398, "compression_ratio": 1.4833333333333334, "no_speech_prob": 1.430511474609375e-05, "words": [{"start": 4370.29, "end": 4370.75, "word": "طبعا", "probability": 0.8248291015625}, {"start": 4370.75, "end": 4371.41, "word": " فهذا", "probability": 0.7679036458333334}, {"start": 4371.41, 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4415.29, "end": 4430.35, "text": "لا يُأخذ بها طبعا mark the increase or decrease in room temperature lead to increase or decrease ESR result احنا حكينا زيادة ال room temperature كل ما زادت درجة الحرارة هتزيد قيمة ال ESR", "tokens": [15040, 7251, 10859, 10721, 9778, 8848, 4724, 11296, 23032, 3555, 3615, 995, 1491, 264, 3488, 420, 11514, 294, 1808, 4292, 1477, 281, 3488, 420, 11514, 12564, 49, 1874, 1975, 5016, 8315, 11331, 4117, 1829, 8315, 30767, 1829, 18513, 3660, 2423, 1808, 4292, 28242, 19446, 30767, 18513, 2655, 11778, 47341, 3660, 21542, 2288, 9640, 3660, 8032, 2655, 11622, 25708, 12174, 32640, 3660, 2423, 12564, 49], "avg_logprob": -0.15336538461538463, "compression_ratio": 1.473053892215569, "no_speech_prob": 7.450580596923828e-06, "words": [{"start": 4415.29, "end": 4415.75, "word": "لا", "probability": 0.8046875}, {"start": 4415.75, "end": 4416.11, "word": " يُأخذ", "probability": 0.7423828125}, {"start": 4416.11, "end": 4416.43, "word": " بها", "probability": 0.79345703125}, 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"probability": 0.970703125}, {"start": 4592.61, "end": 4592.89, "word": " as", "probability": 0.98193359375}, {"start": 4592.89, "end": 4593.05, "word": " the", "probability": 0.73486328125}, {"start": 4593.05, "end": 4593.51, "word": " standard", "probability": 0.9423828125}, {"start": 4593.51, "end": 4593.83, "word": " يعني", "probability": 0.786376953125}, {"start": 4593.83, "end": 4594.01, "word": " هي", "probability": 0.88818359375}, {"start": 4594.01, "end": 4594.19, "word": " ال", "probability": 0.8974609375}, {"start": 4594.19, "end": 4594.59, "word": " standard", "probability": 0.96337890625}, {"start": 4594.59, "end": 4594.95, "word": " method", "probability": 0.98388671875}, {"start": 4594.95, "end": 4595.09, "word": " و", "probability": 0.880859375}, {"start": 4595.09, "end": 4595.63, "word": " المتمدة", "probability": 0.55810546875}, {"start": 4595.63, "end": 4596.11, "word": " أكتر", "probability": 0.8424072265625}, {"start": 4596.11, "end": 4596.77, "word": " الوستيجرين", "probability": 0.8755580357142857}, {"start": 4596.77, "end": 4597.09, "word": " method", "probability": 0.9912109375}], "temperature": 1.0}, {"id": 204, "seek": 461449, "start": 4597.91, "end": 4614.49, "text": "When reading the ESR result on blood with an extremely high YBCs or platelet count, the buffy coat should be excluded نفس فكرة الهيموتقرات لما أنا كنت بقيس الهيموتقرات كنت بقيس نسبة ال RBCs فقط بدون ال buffy coat", "tokens": [11645, 3760, 264, 12564, 49, 1874, 322, 3390, 365, 364, 4664, 1090, 398, 7869, 82, 420, 3403, 15966, 1207, 11, 264, 9204, 88, 10690, 820, 312, 29486, 8717, 36178, 6156, 4117, 25720, 2423, 3224, 32640, 35473, 4587, 2288, 9307, 5296, 15042, 41850, 9122, 29399, 4724, 38436, 3794, 2423, 3224, 32640, 35473, 4587, 2288, 9307, 9122, 29399, 4724, 38436, 3794, 8717, 35457, 3660, 2423, 497, 7869, 82, 6156, 47432, 47525, 11536, 2423, 9204, 88, 10690], "avg_logprob": -0.2121875007947286, "compression_ratio": 1.373134328358209, "no_speech_prob": 0.0, "words": [{"start": 4597.91, "end": 4598.23, "word": "When", "probability": 0.37841796875}, {"start": 4598.23, "end": 4598.67, "word": " reading", "probability": 0.9208984375}, {"start": 4598.67, "end": 4598.89, "word": " the", "probability": 0.9306640625}, {"start": 4598.89, "end": 4599.45, "word": " ESR", "probability": 0.938720703125}, {"start": 4599.45, "end": 4600.03, "word": " result", "probability": 0.73095703125}, {"start": 4600.03, "end": 4600.33, "word": " on", "probability": 0.908203125}, {"start": 4600.33, "end": 4600.77, "word": " blood", "probability": 0.69921875}, {"start": 4600.77, "end": 4601.07, "word": " with", "probability": 0.9052734375}, {"start": 4601.07, "end": 4601.29, "word": " an", "probability": 0.87548828125}, {"start": 4601.29, "end": 4601.87, "word": " extremely", "probability": 0.8017578125}, {"start": 4601.87, "end": 4602.35, "word": " high", "probability": 0.86962890625}, {"start": 4602.35, "end": 4603.27, "word": " YBCs", "probability": 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الهيموتقرات", "probability": 0.59765625}, {"start": 4608.85, "end": 4609.05, "word": " لما", "probability": 0.7122802734375}, {"start": 4609.05, "end": 4609.25, "word": " أنا", "probability": 0.76318359375}, {"start": 4609.25, "end": 4609.57, "word": " كنت", "probability": 0.968505859375}, {"start": 4609.57, "end": 4610.03, "word": " بقيس", "probability": 0.8992513020833334}, {"start": 4610.03, "end": 4610.83, "word": " الهيموتقرات", "probability": 0.9649832589285714}, {"start": 4610.83, "end": 4611.03, "word": " كنت", "probability": 0.951416015625}, {"start": 4611.03, "end": 4611.45, "word": " بقيس", "probability": 0.9337565104166666}, {"start": 4611.45, "end": 4611.87, "word": " نسبة", "probability": 0.9923502604166666}, {"start": 4611.87, "end": 4611.97, "word": " ال", "probability": 0.9140625}, {"start": 4611.97, "end": 4612.77, "word": " RBCs", "probability": 0.818359375}, {"start": 4612.77, "end": 4613.15, "word": " فقط", "probability": 0.982421875}, {"start": 4613.15, "end": 4613.57, "word": " بدون", "probability": 0.994384765625}, {"start": 4613.57, "end": 4613.91, "word": " ال", "probability": 0.73779296875}, {"start": 4613.91, "end": 4614.23, "word": " buffy", "probability": 0.854736328125}, {"start": 4614.23, "end": 4614.49, "word": " coat", "probability": 0.9482421875}], "temperature": 1.0}, {"id": 205, "seek": 464235, "start": 4614.89, "end": 4642.35, "text": "طبعا و ايضا لما بدي اقيس ال ESR الترسيب هشوف مسافة اللي قطعتها يعني مسافة البلازمة او الحجم .. المسافة اللي احتلتها البلازمة طبعا و .. يعني هذا الاشي بدون ما انا اقرأ ال buffy quote يعني should be excluded يعني ال buffy quote يعني بيكون خارج القراءة تبعتي if there is poor separation of the RBCs and the plasma layer", "tokens": [9566, 3555, 3615, 995, 4032, 1975, 1829, 11242, 995, 5296, 15042, 4724, 16254, 1975, 38436, 3794, 2423, 12564, 49, 16712, 2288, 3794, 1829, 3555, 8032, 8592, 38688, 47524, 31845, 3660, 13672, 1829, 12174, 9566, 34268, 11296, 37495, 22653, 47524, 31845, 3660, 29739, 1211, 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4639.25, "word": " is", "probability": 0.92041015625}, {"start": 4639.25, "end": 4639.53, "word": " poor", "probability": 0.89013671875}, {"start": 4639.53, "end": 4640.09, "word": " separation", "probability": 0.9775390625}, {"start": 4640.09, "end": 4640.33, "word": " of", "probability": 0.95654296875}, {"start": 4640.33, "end": 4640.51, "word": " the", "probability": 0.88232421875}, {"start": 4640.51, "end": 4641.13, "word": " RBCs", "probability": 0.85400390625}, {"start": 4641.13, "end": 4641.31, "word": " and", "probability": 0.96484375}, {"start": 4641.31, "end": 4641.45, "word": " the", "probability": 0.7431640625}, {"start": 4641.45, "end": 4641.81, "word": " plasma", "probability": 0.9501953125}, {"start": 4641.81, "end": 4642.35, "word": " layer", "probability": 0.9140625}], "temperature": 1.0}, {"id": 206, "seek": 466924, "start": 4643.66, "end": 4669.24, "text": "this may be due to increased number of reticulocyte و بسمي stratified sedimentation هلأ إيش معنى هذه الجملة؟ هلأ في نوع من الخلائق أو يعني مرحلة من مراحل نضوج ال RBCs بسميها reticulocyte مرحلة ال reticulocyte هي مرحلة ما قبل خروجها مباشرة على ال circulation", "tokens": [11176, 815, 312, 3462, 281, 6505, 1230, 295, 1533, 299, 425, 31078, 975, 4032, 4724, 38251, 1829, 23674, 2587, 32362, 399, 8032, 1211, 10721, 11933, 1829, 8592, 20449, 1863, 7578, 29538, 25724, 2304, 37977, 22807, 8032, 1211, 10721, 8978, 8717, 45367, 9154, 33962, 1211, 16373, 4587, 34051, 37495, 22653, 3714, 2288, 5016, 37977, 9154, 3714, 23557, 5016, 1211, 8717, 11242, 29245, 2423, 497, 7869, 82, 4724, 38251, 1829, 11296, 1533, 299, 425, 31078, 975, 3714, 2288, 5016, 37977, 2423, 1533, 299, 425, 31078, 975, 39896, 3714, 2288, 5016, 37977, 19446, 12174, 36150, 16490, 2288, 29245, 11296, 3714, 3555, 33599, 25720, 15844, 2423, 23168], "avg_logprob": -0.1637620196606104, "compression_ratio": 1.5732758620689655, "no_speech_prob": 1.7881393432617188e-07, "words": [{"start": 4643.66, "end": 4643.98, "word": "this", "probability": 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"probability": 0.973876953125}, {"start": 4650.38, "end": 4651.3, "word": " هلأ", "probability": 0.7154947916666666}, {"start": 4651.3, "end": 4651.48, "word": " إيش", "probability": 0.8385416666666666}, {"start": 4651.48, "end": 4651.8, "word": " معنى", "probability": 0.9073893229166666}, {"start": 4651.8, "end": 4651.98, "word": " هذه", "probability": 0.436767578125}, {"start": 4651.98, "end": 4653.96, "word": " الجملة؟", "probability": 0.87158203125}, {"start": 4653.96, "end": 4654.34, "word": " هلأ", "probability": 0.8575846354166666}, {"start": 4654.34, "end": 4654.76, "word": " في", "probability": 0.74560546875}, {"start": 4654.76, "end": 4655.74, "word": " نوع", "probability": 0.92919921875}, {"start": 4655.74, "end": 4655.98, "word": " من", "probability": 0.98876953125}, {"start": 4655.98, "end": 4656.68, "word": " الخلائق", "probability": 0.8023681640625}, {"start": 4656.68, "end": 4656.9, "word": " أو", "probability": 0.82373046875}, {"start": 4656.9, "end": 4657.2, "word": " 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82, 26118, 411, 8630, 13561], "avg_logprob": -0.22370793727728036, "compression_ratio": 0.847457627118644, "no_speech_prob": 0.0, "words": [{"start": 5265.84, "end": 5266.44, "word": "rolox", "probability": 0.4810791015625}, {"start": 5266.44, "end": 5267.34, "word": " معناها", "probability": 0.9853515625}, {"start": 5267.34, "end": 5270.04, "word": " RBCs", "probability": 0.7799479166666666}, {"start": 5270.04, "end": 5272.26, "word": " aggregate", "probability": 0.7734375}, {"start": 5272.26, "end": 5276.9, "word": " like", "probability": 0.84423828125}, {"start": 5276.9, "end": 5280.62, "word": " stack", "probability": 0.93310546875}, {"start": 5280.62, "end": 5283.02, "word": " coins", "probability": 0.93896484375}], "temperature": 1.0}, {"id": 230, "seek": 530578, "start": 5284.42, "end": 5305.78, "text": "زي القطع المعدنية أو اللي هي أو العملة الملتصقة مع بعضها البعض اللي هي يعني قائص أفطة الشواكر اللي أنا دربتلكم مثال عليها تمام؟ و هيك أيضا بت .. و هيك تعريفها في الكتب يعني RBCs aggregate like stack of coins يعني زي قطع معدنية منزلقة على بعضها البعض أو ملتصقة ببعضها البعض", "tokens": [11622, 1829, 25062, 9566, 3615, 9673, 22488, 1863, 10632, 34051, 13672, 1829, 39896, 34051, 18863, 2304, 37977, 9673, 1211, 2655, 9381, 28671, 20449, 45030, 11242, 11296, 29739, 3615, 11242, 13672, 1829, 39896, 37495, 22653, 12174, 16373, 9381, 5551, 5172, 9566, 3660, 25124, 14407, 4117, 2288, 13672, 1829, 41850, 11778, 25513, 2655, 23275, 2304, 50113, 6027, 25894, 11296, 46811, 10943, 22807, 4032, 39896, 4117, 36632, 11242, 995, 39894, 4386, 4032, 39896, 4117, 37279, 16572, 5172, 11296, 8978, 33251, 2655, 3555, 37495, 22653, 497, 7869, 82, 26118, 411, 8630, 295, 13561, 37495, 22653, 30767, 1829, 12174, 9566, 3615, 20449, 3215, 1863, 10632, 9154, 11622, 1211, 28671, 15844, 45030, 11242, 11296, 29739, 3615, 11242, 34051, 3714, 1211, 2655, 9381, 28671, 4724, 3555, 3615, 11242, 11296, 29739, 3615, 11242], "avg_logprob": -0.13988095498274242, "compression_ratio": 1.897119341563786, "no_speech_prob": 4.0531158447265625e-06, "words": [{"start": 5284.42, "end": 5284.68, "word": "زي", "probability": 0.92333984375}, {"start": 5284.68, "end": 5285.1, "word": " القطع", "probability": 0.9523111979166666}, {"start": 5285.1, "end": 5285.98, "word": " المعدنية", "probability": 0.982177734375}, {"start": 5285.98, "end": 5286.34, "word": " أو", "probability": 0.72900390625}, {"start": 5286.34, "end": 5286.62, "word": " اللي", "probability": 0.89599609375}, {"start": 5286.62, "end": 5286.9, "word": " هي", "probability": 0.931640625}, {"start": 5286.9, "end": 5287.16, "word": " أو", "probability": 0.49853515625}, {"start": 5287.16, "end": 5287.68, "word": " العملة", "probability": 0.9637044270833334}, {"start": 5287.68, "end": 5288.44, "word": " الملتصقة", "probability": 0.9150390625}, {"start": 5288.44, "end": 5288.64, "word": " مع", "probability": 0.9951171875}, {"start": 5288.64, "end": 5289.06, "word": " بعضها", "probability": 0.9866536458333334}, {"start": 5289.06, "end": 5289.8, "word": " البعض", "probability": 0.9283854166666666}, {"start": 5289.8, "end": 5290.18, "word": " اللي", "probability": 0.773681640625}, {"start": 5290.18, "end": 5290.38, "word": " هي", "probability": 0.93798828125}, {"start": 5290.38, "end": 5290.9, "word": " يعني", "probability": 0.952392578125}, {"start": 5290.9, "end": 5291.56, "word": " قائص", "probability": 0.63427734375}, {"start": 5291.56, "end": 5291.86, "word": " أفطة", "probability": 0.697998046875}, {"start": 5291.86, "end": 5292.38, "word": " الشواكر", "probability": 0.7486572265625}, {"start": 5292.38, "end": 5292.52, "word": " اللي", "probability": 0.96044921875}, {"start": 5292.52, "end": 5292.76, "word": " أنا", "probability": 0.7578125}, {"start": 5292.76, "end": 5293.84, "word": " دربتلكم", "probability": 0.84228515625}, {"start": 5293.84, "end": 5294.18, "word": " مثال", "probability": 0.98779296875}, {"start": 5294.18, "end": 5294.72, "word": " عليها", "probability": 0.97705078125}, {"start": 5294.72, "end": 5295.48, "word": " تمام؟", "probability": 0.7791341145833334}, {"start": 5295.48, "end": 5295.56, "word": " و", "probability": 0.62646484375}, {"start": 5295.56, "end": 5295.74, "word": " هيك", "probability": 0.957763671875}, {"start": 5295.74, "end": 5296.04, "word": " أيضا", "probability": 0.9152018229166666}, {"start": 5296.04, "end": 5296.22, "word": " بت", "probability": 0.849609375}, {"start": 5296.22, "end": 5296.6, "word": " ..", "probability": 0.77685546875}, {"start": 5296.6, "end": 5296.88, "word": " و", "probability": 0.484130859375}, {"start": 5296.88, "end": 5297.34, "word": " هيك", "probability": 0.9111328125}, {"start": 5297.34, "end": 5297.76, "word": " تعريفها", "probability": 0.9156494140625}, {"start": 5297.76, "end": 5297.88, "word": " في", "probability": 0.9384765625}, {"start": 5297.88, "end": 5298.28, "word": " الكتب", "probability": 0.9830729166666666}, {"start": 5298.28, "end": 5298.68, "word": " يعني", "probability": 0.73583984375}, {"start": 5298.68, "end": 5299.7, "word": " RBCs", "probability": 0.7535807291666666}, {"start": 5299.7, "end": 5300.1, "word": " aggregate", "probability": 0.75537109375}, {"start": 5300.1, "end": 5300.46, "word": " like", "probability": 0.6845703125}, {"start": 5300.46, "end": 5300.86, "word": " stack", "probability": 0.65380859375}, {"start": 5300.86, "end": 5301.06, "word": " of", "probability": 0.97412109375}, {"start": 5301.06, "end": 5301.38, "word": " coins", "probability": 0.93115234375}, {"start": 5301.38, "end": 5301.64, "word": " يعني", "probability": 0.926513671875}, {"start": 5301.64, "end": 5301.82, "word": " زي", "probability": 0.986083984375}, {"start": 5301.82, "end": 5302.08, "word": " قطع", "probability": 0.9171549479166666}, {"start": 5302.08, "end": 5302.58, "word": " معدنية", "probability": 0.9815673828125}, {"start": 5302.58, "end": 5303.46, "word": " منزلقة", "probability": 0.800537109375}, {"start": 5303.46, "end": 5303.64, "word": " على", "probability": 0.8515625}, {"start": 5303.64, "end": 5304.0, "word": " بعضها", "probability": 0.9889322916666666}, {"start": 5304.0, "end": 5304.28, "word": " البعض", "probability": 0.9934895833333334}, {"start": 5304.28, "end": 5304.44, "word": " أو", "probability": 0.8681640625}, {"start": 5304.44, "end": 5304.92, "word": " ملتصقة", "probability": 0.95703125}, {"start": 5304.92, "end": 5305.38, "word": " ببعضها", "probability": 0.97568359375}, {"start": 5305.38, "end": 5305.78, "word": " البعض", "probability": 0.9973958333333334}], "temperature": 1.0}, {"id": 231, "seek": 531401, "start": 5308.59, "end": 5314.01, "text": "وصلنا لنهاية محاضرتنا لليوم، يعطيكم ألف عافية وموفقين بإبن الله", "tokens": [2407, 36520, 8315, 5296, 1863, 11296, 10632, 3714, 5016, 46958, 43500, 8315, 5296, 20292, 20498, 12399, 37495, 9566, 1829, 24793, 5551, 46538, 6225, 31845, 10632, 4032, 2304, 38688, 4587, 9957, 4724, 28814, 3555, 1863, 21984], "avg_logprob": -0.11545138744016488, "compression_ratio": 1.2717391304347827, "no_speech_prob": 6.556510925292969e-07, "words": [{"start": 5308.59, "end": 5309.63, "word": "وصلنا", "probability": 0.935546875}, {"start": 5309.63, "end": 5310.17, "word": " لنهاية", "probability": 0.9228515625}, {"start": 5310.17, "end": 5310.89, "word": " محاضرتنا", "probability": 0.94619140625}, {"start": 5310.89, "end": 5311.49, "word": " لليوم،", "probability": 0.79388427734375}, {"start": 5311.49, "end": 5311.95, "word": " يعطيكم", "probability": 0.9371337890625}, {"start": 5311.95, "end": 5312.23, "word": " ألف", "probability": 0.74853515625}, {"start": 5312.23, "end": 5312.73, "word": " عافية", "probability": 0.9840494791666666}, {"start": 5312.73, "end": 5313.41, "word": " وموفقين", "probability": 0.89462890625}, {"start": 5313.41, "end": 5313.75, "word": " بإبن", "probability": 0.904052734375}, {"start": 5313.75, "end": 5314.01, "word": " الله", "probability": 0.98095703125}], "temperature": 1.0}], "language": "ar", "language_probability": 1.0, "duration": 5315.3265, "duration_after_vad": 5146.220000000012}
\ No newline at end of file
diff --git a/PL9fwy3NUQKwY9jl8xw-bWkVZkWcGGyspd/Bu-y4fC_shI_raw.srt b/PL9fwy3NUQKwY9jl8xw-bWkVZkWcGGyspd/Bu-y4fC_shI_raw.srt
new file mode 100644
index 0000000000000000000000000000000000000000..0cc61a5619a8d04a93c4d7971ebb487d51d22172
--- /dev/null
+++ b/PL9fwy3NUQKwY9jl8xw-bWkVZkWcGGyspd/Bu-y4fC_shI_raw.srt
@@ -0,0 +1,4416 @@
+1
+00:00:02,830 --> 00:00:06,730
+السلام عليكم ورحمة الله وبركاته طالبات العزيزات
+
+2
+00:00:06,730 --> 00:00:09,970
+اليوم ان شاء الله راح نشرح محاضرة erythrocyte
+
+3
+00:00:09,970 --> 00:00:14,570
+sedimentation rate erythrocyte معناها red blood
+
+4
+00:00:14,570 --> 00:00:19,980
+cell خلايا الدم الحمراء sedimentationمعناها ترسيب
+
+5
+00:00:19,980 --> 00:00:25,240
+rate معدل، إذا ما عملنا اليوم بعنوان erythrocyte
+
+6
+00:00:25,240 --> 00:00:29,500
+sedimentation rate أي معدل ترسيب خلايا الدم
+
+7
+00:00:29,500 --> 00:00:35,900
+الحمراء، فيما يعرف بـ ESR تمام؟ اختصار لإله ESRقبل
+
+8
+00:00:35,900 --> 00:00:39,600
+ما نعرض ال slides خلونا بس مع بعض نشوف هاي الصورة
+
+9
+00:00:39,600 --> 00:00:43,340
+و هاي الصورة طبعا مش غريبة علينا يا بنات احنا
+
+10
+00:00:43,340 --> 00:00:47,560
+عرضناها في المعامل السابقة و تحدثنا عن ال blood
+
+11
+00:00:47,560 --> 00:00:51,140
+component حكينا لو انا عندي عينة blood في tube
+
+12
+00:00:51,140 --> 00:00:55,620
+وفصلتها عملتلها separation on centrifuge باستخدام
+
+13
+00:00:55,620 --> 00:01:00,900
+جهاز الطرد المركزي فرح تفصل العينة الى 3 طبقات
+
+14
+00:01:01,150 --> 00:01:04,170
+الطبقة اللى بتكون موجودة في البطم هي طبقة ال red
+
+15
+00:01:04,170 --> 00:01:08,790
+blood cell على أساس إنها هي ال red blood cell أو
+
+16
+00:01:08,790 --> 00:01:15,710
+ال RBCs على أساس إن هاي الطبقة بتكون الأسقل تمام و
+
+17
+00:01:15,710 --> 00:01:22,030
+تحتل ما بين 44 إلى 45% من ال total volume of whole
+
+18
+00:01:22,030 --> 00:01:26,690
+bloodو يليها طبقة ال buffy coat اللى هى الطبقة
+
+19
+00:01:26,690 --> 00:01:31,570
+الكريمية اللى بتكون مكونة من او يعني تحتوي على ال
+
+20
+00:01:31,570 --> 00:01:35,650
+white blood cell and the platelet طبعا خلايا الدم
+
+21
+00:01:35,650 --> 00:01:41,370
+البيضاء و الصفائح الدموية هذه الطبقة لا تتجاوز ال
+
+22
+00:01:41,370 --> 00:01:46,450
+1% طبعا او احيانا نحكي تجاوزا نحكي انها 1% من ال
+
+23
+00:01:46,450 --> 00:01:51,590
+total blood volume او ال whole blood volumeطيب و
+
+24
+00:01:51,590 --> 00:01:57,850
+يلي هاي طبقة طبقة ال plasma اللى بتحتال 55% من ال
+
+25
+00:01:57,850 --> 00:02:01,730
+holy blood volume و هى طبعا بتكون موجودة at the
+
+26
+00:02:01,730 --> 00:02:06,600
+top على أساس أنها هى أخف طبقة طبعا؟طيب بس اليوم
+
+27
+00:02:06,600 --> 00:02:09,920
+انا مش عم بذكر هاي ال .. ال .. او مش عم بعرض هاي
+
+28
+00:02:09,920 --> 00:02:17,680
+الصورة لحتى فقط نذكر مكونات ال .. ال .. الدم لأ
+
+29
+00:02:17,680 --> 00:02:23,280
+انا اليوم بدي .. بدي احكي انه عينت الدم طالما انه
+
+30
+00:02:23,280 --> 00:02:27,960
+انا ساحبتها و عملتلها mixing يعني وضعتها في tube و
+
+31
+00:02:27,960 --> 00:02:32,240
+عملتلها mixing مع anticoagulantAnticoagulant يعني
+
+32
+00:02:32,240 --> 00:02:37,580
+مانع للتخثر سواء كان هذا مانع التخثر edita أو
+
+33
+00:02:37,580 --> 00:02:42,420
+citrate أو heparin أو أي نوع من أنواع موانع التخثر
+
+34
+00:02:42,420 --> 00:02:46,840
+اللي تم ذكرها أو يعني موجودة في محاضرة ل blood
+
+35
+00:02:46,840 --> 00:02:53,290
+componentتمام؟ إذا تم سحب عينة وعملنا لها mixing
+
+36
+00:02:53,290 --> 00:02:57,690
+مع anticoagulant يعني منعنا تخثر هذه العينة، منعنا
+
+37
+00:02:57,690 --> 00:03:02,130
+تجلطها، إذا يبقى الدم في الصورة السائلة لإله وإذا
+
+38
+00:03:02,130 --> 00:03:06,530
+بقيت دم في الصورة السائلة لإله، فهو يا بنات، يعني
+
+39
+00:03:06,530 --> 00:03:10,230
+tend to يميل إلى أنه يفصل لو أنا حطيته على الbench
+
+40
+00:03:10,230 --> 00:03:14,490
+لمدة يعني عشر دقائق، ربع ساعة، بيكون بلش يفصلطبعا
+
+41
+00:03:14,490 --> 00:03:20,530
+أنا بستخدم ال centrifuge لحتى أسرع العملية هذه
+
+42
+00:03:20,530 --> 00:03:24,810
+دقيقة تان تلاتة خمس دقائق أنا بكون انتهت من عملية
+
+43
+00:03:24,810 --> 00:03:30,930
+الفصل طبعا لكن ال blood في الصورة السائلة لإله هو
+
+44
+00:03:30,930 --> 00:03:35,230
+يميل إلى أنه يفصل بحيث أن ال RBC بفعل الجاذبية
+
+45
+00:03:35,230 --> 00:03:39,770
+بتترسب لتحت و بتفصل ل plasma at the top يعني بشكل
+
+46
+00:03:39,770 --> 00:03:44,060
+طبيعي بس بدها تاخد وقت هذه العملية طبعاو هذه هي
+
+47
+00:03:44,060 --> 00:03:50,760
+فكرة فحصنا لليوم يعني فكرة فحصنا لليوم بتحكي انه
+
+48
+00:03:50,760 --> 00:03:56,500
+خلينا بصناعد الصورة هاي ال ESR الاريثروسايت
+
+49
+00:03:56,500 --> 00:04:00,880
+سيديمنتاشن ريت فكرة الفحص طبعه انه انا باستخدام
+
+50
+00:04:00,880 --> 00:04:05,420
+انابيب خاصة يا بنات و ركزولي على كلمة انابيب خاصة
+
+51
+00:04:05,420 --> 00:04:08,700
+راح نذكر ايش أنواع الانابيب اللي انا بستخدمها
+
+52
+00:04:08,700 --> 00:04:14,800
+لاحقا ولكنباستخدام أنابيب خاصة أنا بجمع عينة عينة
+
+53
+00:04:14,800 --> 00:04:19,900
+holy blood دم و بعملها mixing مع anticoagulant
+
+54
+00:04:19,900 --> 00:04:23,440
+يفضل إن أنا أستخدم ال citrate ال sodium citrate
+
+55
+00:04:23,440 --> 00:04:28,700
+anticoagulant و بعد هيك أنا بحطها على ال bench
+
+56
+00:04:28,700 --> 00:04:33,400
+بشكل عمودي تمام؟ بشكل عمودي يعني إنها تصنع مع ال
+
+57
+00:04:33,400 --> 00:04:38,240
+bench زاوية 90 درجة تمام؟ لازم تكون vertical أو
+
+58
+00:04:38,240 --> 00:04:41,410
+perpendicular to the benchتمام؟ بحطها في راك و
+
+59
+00:04:41,410 --> 00:04:45,590
+الراك بأضمن أنها هي .. هاي ال .. ال .. النبوبة بت
+
+60
+00:04:45,590 --> 00:04:51,390
+.. بشكل .. باتجاه عمودي و تصنع زاوية 90 درجة مع
+
+61
+00:04:51,390 --> 00:04:56,270
+المستوى اللي .. اللي هي موجودة عليه و بستن عليها
+
+62
+00:04:56,270 --> 00:05:00,600
+ساعةلأن حكينا اللي هو ال blood في صورته السائلة
+
+63
+00:05:00,600 --> 00:05:05,440
+يميل إلى إنه يفصل فال RBCs راح تتجه إلى ال bottom
+
+64
+00:05:05,440 --> 00:05:13,160
+و ال plasma راح تفصل بالنسبة للقراقة أو النتيجة
+
+65
+00:05:13,160 --> 00:05:18,520
+اللي أنا بدي أقراها أنا طبعا بقيس أو يعنيبدي قراءة
+
+66
+00:05:18,520 --> 00:05:23,340
+rate معدل يعني سرعة تمام؟ و احنا يمكن أخدنا
+
+67
+00:05:23,340 --> 00:05:28,220
+بالفيزيا ان السرعة هي عبارة عن المسافة على الزمن
+
+68
+00:05:28,220 --> 00:05:34,500
+تمام؟ طيب، اذا طبعا انا بما اني بدي معدل ترسيب ال
+
+69
+00:05:34,500 --> 00:05:38,820
+RBCs اذا انا بدي المسافة اللي تقطعها ال RBCs لما
+
+70
+00:05:38,820 --> 00:05:44,180
+بتترسب على الزمن تمام؟ اذا انا بقيس ال plasma
+
+71
+00:05:45,090 --> 00:05:50,210
+volume أو مسافة ال plasma هذه المسافة أنا بقيسها و
+
+72
+00:05:50,210 --> 00:05:54,870
+بقسمها على الزمن هذه المسافة يا بنات كانت مشغولة
+
+73
+00:05:54,870 --> 00:06:01,410
+بال RBC لما كان ال blood well mixed يعني ماعمله
+
+74
+00:06:01,410 --> 00:06:07,390
+mixing منيحتمام؟ بمجرد انه ترى السبق يعني ال RBCs
+
+75
+00:06:07,390 --> 00:06:10,110
+قطعت هذه المسافة كلها لحتى صارت في ال sediment
+
+76
+00:06:10,110 --> 00:06:15,450
+تمام؟ اذا انا بدي اقيس هذه المسافة على الزمن على
+
+77
+00:06:15,450 --> 00:06:18,110
+العكس من ال hematocrite ال hematocrite انا ايش كنت
+
+78
+00:06:18,110 --> 00:06:22,770
+اعمل؟ كنت اقيس نسبة ال .. ال RBCs يعني تمام؟
+
+79
+00:06:22,770 --> 00:06:26,090
+بالنسبة لل whole blood volume اعتبرها انها هي 100%
+
+80
+00:06:26,090 --> 00:06:30,890
+وشوف كام نسبة ال RBCs منها في الميةولكن في ال ESR
+
+81
+00:06:30,890 --> 00:06:35,510
+لأ أنا بقيس المسافة ل plasma على ال .. ال .. الزمن
+
+82
+00:06:35,510 --> 00:06:40,090
+اللي هي one hour تمام؟ اذا هذه هي فكرة فحصنا لليوم
+
+83
+00:06:40,090 --> 00:06:43,890
+علشان لما نشرح كمان شوية او نبلش بالشرح بعرض ال
+
+84
+00:06:43,890 --> 00:06:50,430
+slides انكون على الأقل متخيلين ايش يعني فحص ال ESR
+
+85
+00:06:51,000 --> 00:06:54,160
+Erythrocyte Sedimentation Rate حكينا إنه أنا
+
+86
+00:06:54,160 --> 00:06:59,500
+باستخدام أنابيب خاصة بملأها بيه ال blood اللي well
+
+87
+00:06:59,500 --> 00:07:04,280
+mixed تمام صارله mixing منيح مع anticoagulant و
+
+88
+00:07:04,280 --> 00:07:10,380
+طبعا بملأ هاي الأنابيب و بجلسها بشكل عمودي تمام
+
+89
+00:07:10,380 --> 00:07:14,820
+على راجوطبعا بيكون في خلفية ال rack فيه تدريج و
+
+90
+00:07:14,820 --> 00:07:18,800
+بعد ساعة مرور ساعة من الزمن أنا بقيس المسافة اللي
+
+91
+00:07:18,800 --> 00:07:22,600
+قطعتها ال RBC يعني مسافة البلازما هاي فكرة الفحص
+
+92
+00:07:22,600 --> 00:07:27,020
+طبعا الاريثروسيت سيديمنتاشن ريت الاريثروسيت
+
+93
+00:07:27,020 --> 00:07:32,740
+سيديمنتاشن ريت ESR is a non specific measurement
+
+94
+00:07:32,740 --> 00:07:37,640
+used to detect and monitoran inflammatory response
+
+95
+00:07:37,640 --> 00:07:41,740
+to tissue injury and acute phase in which there is
+
+96
+00:07:41,740 --> 00:07:44,400
+a change in plasma concentration of several
+
+97
+00:07:44,400 --> 00:07:48,440
+proteins ما معنى هذا الكلام؟ هلأ ال erythrocyte
+
+98
+00:07:48,440 --> 00:07:52,920
+sedimentation rate فحص ال ESR هو عبارة عن فحص
+
+99
+00:07:52,920 --> 00:07:59,680
+يستخدم used to يستخدم ليه؟ لحتى أعمل detection and
+
+100
+00:07:59,680 --> 00:08:04,790
+monitoring لمين؟ لل inflammatory responseإذا كان
+
+101
+00:08:04,790 --> 00:08:09,670
+في .. في الجسم inflammation أو tissue injury أو
+
+102
+00:08:09,670 --> 00:08:14,210
+infection تمام؟ ممكن أنا أعمل detection تنبؤ عن
+
+103
+00:08:14,210 --> 00:08:18,230
+وجود هاي ال .. ال infection أو ال inflammation أو
+
+104
+00:08:18,230 --> 00:08:21,950
+ال tissue injury ممكن أعمل monitoring أشوف الوضع
+
+105
+00:08:21,950 --> 00:08:28,160
+بتحسن مع الهلاج أو بيزيد سوء مثلا؟تمام؟ بعمل
+
+106
+00:08:28,160 --> 00:08:33,600
+monitoring مراقبة لحالة المريض من خلال فحص ال ESR
+
+107
+00:08:33,600 --> 00:08:37,560
+المريض اللي بيكون عنده إما inflammation أو tissue
+
+108
+00:08:37,560 --> 00:08:43,100
+injury أو حتى infection تمام؟ طيب ولكن هذا الفحص
+
+109
+00:08:43,100 --> 00:08:48,400
+is non specific يعني مثلا إذا كان نتيجة ال ESR
+
+110
+00:08:48,400 --> 00:08:52,160
+عالية بيكون يعني في عندي inflammation أو infection
+
+111
+00:08:52,160 --> 00:08:57,460
+أو tissue injury طيبخلاص أنا بعرف إنه في
+
+112
+00:08:57,460 --> 00:09:02,840
+inflammation لكن هل ب .. يعني بإمكاني أحدد وين ال
+
+113
+00:09:02,840 --> 00:09:07,100
+infection أو وين ال inflammation في القلب، في ال
+
+114
+00:09:07,100 --> 00:09:10,460
+kidney، في ال liver، في الدم؟ وين بالظبط؟ أنا
+
+115
+00:09:10,460 --> 00:09:15,390
+مابقدر أحددهاتمام؟ إذا لا أستطيع تحديد مكان ال
+
+116
+00:09:15,390 --> 00:09:18,150
+infection أو ال inflammation عشان هيك هو non
+
+117
+00:09:18,150 --> 00:09:23,590
+-specific مش متخصص فحص غير متخصص ولكن هو فحص لل
+
+118
+00:09:23,590 --> 00:09:28,910
+screening تمام؟ للتشييك لحتى أشيك لحتى أكشف أعمل
+
+119
+00:09:28,910 --> 00:09:33,510
+detection and monitoring لل inflammation و ال
+
+120
+00:09:33,510 --> 00:09:39,790
+tissue injury و طبعا يا بناتلأنه في حالة ال
+
+121
+00:09:39,790 --> 00:09:42,330
+inflammation و ال tissue injury و ال infection
+
+122
+00:09:42,330 --> 00:09:48,010
+بيحدث أو بيتم إنتاج بروتينات أو بيزيد تركيز
+
+123
+00:09:48,010 --> 00:09:52,850
+بروتينات في الدم، في ال plasma، بنسميها acute face
+
+124
+00:09:52,850 --> 00:09:57,010
+protein، تمام؟ بنسميها ال acute face protein طبعا
+
+125
+00:09:57,010 --> 00:10:01,990
+هذه ال acute face protein بيطلعب دور مهم جدا في
+
+126
+00:10:01,990 --> 00:10:08,580
+تسريع ترسيب ال RBCsطبعا فإذا وجود ال acute face of
+
+127
+00:10:08,580 --> 00:10:12,880
+protein دلالة على وجود acute face of inflammation
+
+128
+00:10:12,880 --> 00:10:18,580
+أو tissue injury أو infection إذا ESR ماله بيكون
+
+129
+00:10:18,580 --> 00:10:24,300
+قيمته عاليةطيب this procedure very simply consist
+
+130
+00:10:24,300 --> 00:10:29,500
+of allowing specific amount of blood to sit in
+
+131
+00:10:29,500 --> 00:10:33,000
+vertical position for a period of time يعني
+
+132
+00:10:33,000 --> 00:10:38,300
+وظيفتها ببساطة أو فكرة الفحص هذا ببساطة انه انا
+
+133
+00:10:38,300 --> 00:10:44,660
+اخلي عينة الدم انها تكون موجودة بشكل عمودي لمدة
+
+134
+00:10:44,660 --> 00:10:50,100
+زمنية معينة يعني one hour مثلا او عادة يعني عادة
+
+135
+00:10:50,190 --> 00:10:56,030
+نستخدمها يعني التوقيت one hour وطبعا أنا بقيس ال
+
+136
+00:10:56,030 --> 00:10:58,890
+distance اللي هي المسافة اللي قطعتها ال RBCs اللي
+
+137
+00:10:58,890 --> 00:11:04,210
+هي مسافة البلازمة تمام؟ بالmillimeter per hour إذا
+
+138
+00:11:04,210 --> 00:11:10,140
+الوحدة القياس هتكون millimeter per hourThe ESR is
+
+139
+00:11:10,140 --> 00:11:15,740
+affected by three factors ال ESR هذا بيتأثر بتلت
+
+140
+00:11:15,740 --> 00:11:22,820
+عوامل هناك تلاتة players بيلعبوا دور مهم جدا في
+
+141
+00:11:22,820 --> 00:11:29,040
+نتيجة ال ESR أو قيمة ال ESRأول إيش الارتروسايت
+
+142
+00:11:29,040 --> 00:11:32,220
+اللي .. اللي أصلا مفعول بيه أو اللي هي عبتصري لها
+
+143
+00:11:32,220 --> 00:11:36,140
+sedimentation أهم player عندى أيضا ال plasma
+
+144
+00:11:36,140 --> 00:11:40,660
+composition حكينا مكونات البلازمة البلازمة بروتين
+
+145
+00:11:40,660 --> 00:11:46,140
+ال acute face protein اللي بتظهر أو بيزيد تركيزها
+
+146
+00:11:46,140 --> 00:11:49,740
+في حالات ال inflammation و ال infection و ال
+
+147
+00:11:49,740 --> 00:11:54,800
+tissue injury طبعاهذه البروتينات موجودة في
+
+148
+00:11:54,800 --> 00:11:57,660
+البلازمة إذا المكونات البلازمة البلازمة
+
+149
+00:11:57,660 --> 00:12:05,100
+composition مهمة جدا لحتى يعني نشوف كيف بتأثر على
+
+150
+00:12:05,100 --> 00:12:08,700
+ال ESR أيضا ال mechanical و ال technical factors
+
+151
+00:12:08,700 --> 00:12:13,660
+عوامل يعني لها علاقة بشغلي كيف أنا جمعت العينة كيف
+
+152
+00:12:13,660 --> 00:12:16,780
+عملت لها mixing مع ال anticoagulant كيف اشتغلت
+
+153
+00:12:16,780 --> 00:12:20,300
+الفحص البيئة و الظروف اللي اشتغلت فيها الفحص هاي
+
+154
+00:12:20,300 --> 00:12:23,800
+ممكن يأثر على النتيجة راح نشوف كيفلاحقا في ال
+
+155
+00:12:23,800 --> 00:12:28,960
+slides التالية خلينا نشوف أول player عندي اللي هي
+
+156
+00:12:28,960 --> 00:12:34,720
+ال erythrocyte erythrocyte is a factor of chief
+
+157
+00:12:34,720 --> 00:12:38,240
+importance in determining the distance that the
+
+158
+00:12:38,240 --> 00:12:43,320
+RBCs fall تمام؟بحكيلي إنه ال .. ال .. ال
+
+159
+00:12:43,320 --> 00:12:46,300
+erythrocyte .. ال erythrocyte يعني هي عبارة عن
+
+160
+00:12:46,300 --> 00:12:52,500
+عامل مهم جدا في التأثير على نتيجة ال ESR ولعله إنه
+
+161
+00:12:52,500 --> 00:13:00,200
+أهم إشي بال RBCs هو حجمها ال size تبعها و ال mass
+
+162
+00:13:00,200 --> 00:13:05,520
+تبعتها تمام؟ هو اللي بيتحكم بمعدل سقوتها أو معدل
+
+163
+00:13:05,520 --> 00:13:12,210
+ترسيبها في الأنابيبthe larger the particle, the
+
+164
+00:13:12,210 --> 00:13:16,550
+faster its rate of fall طبعا يا بنات لو ال slides
+
+165
+00:13:16,550 --> 00:13:21,390
+عندكم المعلومة هي موجودة مختلفة فياريت تنتبهولها و
+
+166
+00:13:21,390 --> 00:13:24,950
+تعدلوها the larger the particle, the faster its
+
+167
+00:13:24,950 --> 00:13:29,090
+rate of fall طبعا يعني كل ما كبرت خلية ال RBC
+
+168
+00:13:29,090 --> 00:13:36,670
+بيسرع من سقوتها من معدل سقوتهاتمام طيب في عندي
+
+169
+00:13:36,670 --> 00:13:44,570
+أمراض ممكن تسبب ال RBCs size أكبر من الطبيعي وفي
+
+170
+00:13:44,570 --> 00:13:49,910
+عندي أمراض ممكن تتسبب بإنه ال RBCs يكون حجمها أقل
+
+171
+00:13:49,910 --> 00:13:55,450
+من الطبيعي فالأمراض اللي بتسببلي خلايا RBCs حجمها
+
+172
+00:13:55,450 --> 00:14:00,070
+أكبر من الطبيعي يعني larger particle هذا هيكون ال
+
+173
+00:14:00,070 --> 00:14:05,570
+ESR أشماله عندها عاليلأنه faster rate of fall،
+
+174
+00:14:05,570 --> 00:14:12,470
+تمام؟ طيب، أما إذا كانت ال .. ال erythrocyte حجمها
+
+175
+00:14:12,470 --> 00:14:17,930
+صغير فمعنها هذا the slower rate to fall طيب، خليني
+
+176
+00:14:17,930 --> 00:14:21,490
+أنشوف في الوضع الطبيعي، في الوضع الطبيعي بيحكيلي
+
+177
+00:14:21,490 --> 00:14:26,920
+إن ال normal blood في الدم الطبيعيThe RBCs remain
+
+178
+00:14:26,920 --> 00:14:32,060
+more or less separated. They are negatively
+
+179
+00:14:32,060 --> 00:14:37,020
+charged and therefore rebel each other خلايا ال
+
+180
+00:14:37,020 --> 00:14:42,880
+RBCs موجودة على سطحها carbohydrate اسمها سيالك
+
+181
+00:14:42,880 --> 00:14:46,020
+أسد، نوع من أنواع ال carbohydrate اللي اسمه سيالك
+
+182
+00:14:46,020 --> 00:14:50,980
+أسديكسب خلية ال RBCs، تمام يا بنات؟ يكسبها
+
+183
+00:14:50,980 --> 00:14:55,160
+negative charge، اذا خلايا ال RBCs مشحونة من
+
+184
+00:14:55,160 --> 00:15:01,280
+الخارج بشحنة سالبة، negative charge، تمام؟ طيب
+
+185
+00:15:01,930 --> 00:15:05,690
+فخلايا الاربسيز ال negative charge اللي على سطحها
+
+186
+00:15:05,690 --> 00:15:10,370
+بتخليها separated عن بعضها البعض يعني في على
+
+187
+00:15:10,370 --> 00:15:14,170
+مسافات ثابتة من بعضها البعض او في متباعدة هي بتكون
+
+188
+00:15:14,170 --> 00:15:19,850
+مالها متباعدة في ما بينها قوة تنافر repulsion
+
+189
+00:15:19,850 --> 00:15:24,530
+forces ال repulsion forces هذه قوات تنافر بينشأ
+
+190
+00:15:24,530 --> 00:15:28,570
+عنها فرق جهد ما بين الخلايا بسميه zeta potential
+
+191
+00:15:29,200 --> 00:15:33,740
+هذا فرق الجهد اللي اسمه zeta potential ناتج من ال
+
+192
+00:15:33,740 --> 00:15:37,420
+revolution forces اللي هو قوات التنافر والشحنات
+
+193
+00:15:37,420 --> 00:15:41,060
+السالبة اللي موجودة على سطح خلايا ال RBCs فهذا
+
+194
+00:15:41,060 --> 00:15:45,440
+الفرق الجهد بيخلي الخلايا أشمالها separated بعيدة
+
+195
+00:15:45,440 --> 00:15:50,880
+عن بعضها البعضطبعا؟ طيب خلّينا بس نشوف نستعرض صورة
+
+196
+00:15:50,880 --> 00:15:55,640
+و لحتى نعرف إيش يعني إن ال RBC بتكون negatively
+
+197
+00:15:55,640 --> 00:15:58,960
+charged كيف بتكون متباعدها؟ يعني بتكون على سطحها
+
+198
+00:15:58,960 --> 00:16:03,560
+شحنة سالبة هذا بيخليها على مسافة معينة من الخلايا
+
+199
+00:16:03,560 --> 00:16:06,700
+المجاورة لإلها إذا الخلايا ال RBC ما بتكونش إيش
+
+200
+00:16:06,700 --> 00:16:09,860
+ملتصقة مع بعض البعض في العينة أو حتى في ال
+
+201
+00:16:09,860 --> 00:16:14,560
+circulation في الدمتمام؟ بتكون على مسافة معينة من
+
+202
+00:16:14,560 --> 00:16:20,640
+بعضها البعض طبعا هذه الخلايا في مابينها قوة تنافر
+
+203
+00:16:20,640 --> 00:16:24,760
+و أيضا zeta potential فرق جهد اسمه zeta potential
+
+204
+00:16:24,760 --> 00:16:30,400
+بي .. بي .. يعني بيضمن لإن ال RBCs تكون اشمالها
+
+205
+00:16:30,400 --> 00:16:37,450
+متباعدة عن بعضها البعضطبعا؟ طيب فاللي بيصير في
+
+206
+00:16:37,450 --> 00:16:42,790
+الترسيب أنا عندى نسبة البروتينات قليلة جدا فى الدم
+
+207
+00:16:42,790 --> 00:16:45,810
+يعنى وضعه الطبيعى انها تكون قليلة ال acute facet
+
+208
+00:16:45,810 --> 00:16:49,270
+protein طبعا؟ لإن فى الوضع الطبيعى احنا بنحكى يعنى
+
+209
+00:16:49,270 --> 00:16:53,990
+فش inflammation يعنى فش infection طبعا؟ فبالتالي
+
+210
+00:16:53,990 --> 00:16:58,850
+فش عندي acute facet protein بتركيز عالى عندي الوضع
+
+211
+00:16:58,850 --> 00:17:02,820
+الطبيعى او التركيز الطبيعى من هى البروتيناتفإيش
+
+212
+00:17:02,820 --> 00:17:06,160
+بتروح بتعمل هاي البروتينات؟ خلّينا نشوف في الوضع
+
+213
+00:17:06,160 --> 00:17:09,960
+الطبيعي الحد
+
+214
+00:17:09,960 --> 00:17:13,640
+الأدنى من البروتينات هذه أو الحد الطبيعي بيجي
+
+215
+00:17:13,640 --> 00:17:17,760
+وبيلتف حوالين ال RBC زي هيك وإحنا في ال tube وإحنا
+
+216
+00:17:17,760 --> 00:17:21,300
+مجلسينها بشكل عمودي لمدة ساعة بيجي ال ..
+
+217
+00:17:21,300 --> 00:17:25,000
+البروتينات و هي بتعادل شحنة ال erythrocyte بهذا
+
+218
+00:17:25,000 --> 00:17:30,070
+الشكلطبعا طيب بما أنها عادلة الشحنة إذا انقلت ال
+
+219
+00:17:30,070 --> 00:17:34,250
+rebellion forces ما بين ال RBCs إذا ال RBCs هتقترب
+
+220
+00:17:34,250 --> 00:17:39,050
+من بعضها البعض هذا .. هذا يعني number one طبعا
+
+221
+00:17:39,050 --> 00:17:44,510
+number two المسافة ما بين ال RBCs و أختها انشغلت
+
+222
+00:17:44,510 --> 00:17:49,190
+ببروتينات هذا رح يقدي إلى إيش crowding تزاهم ما
+
+223
+00:17:49,190 --> 00:17:54,400
+بين ال RBCsفيعني مدام صار في عندي crowding أو
+
+224
+00:17:54,400 --> 00:17:57,860
+تزاحم ما بين ال RBC أو تقارب ما بينها خلينا نسمي
+
+225
+00:17:57,860 --> 00:18:01,920
+تقارب ما بينهم اذا صار في تجمعات من .. ما بين ال
+
+226
+00:18:01,920 --> 00:18:08,360
+RBCs اذا طالما صار في عندي تجمعات من ال RBCs يعني
+
+227
+00:18:08,360 --> 00:18:13,120
+صار في عندها .. عندي larger particle كبير حجمها
+
+228
+00:18:13,120 --> 00:18:19,150
+اذا تسقط تمام بيصير لها sedimentationطيب، هذا
+
+229
+00:18:19,150 --> 00:18:23,890
+الوضع الطبيعي احنا بنحكي، طبعا؟ طيب خلينا نشوف لو
+
+230
+00:18:23,890 --> 00:18:29,660
+.. لوفي عندي certain disease لو في حالة certain
+
+231
+00:18:29,660 --> 00:18:34,720
+disease ال plasma protein concentration و may be
+
+232
+00:18:34,720 --> 00:18:39,900
+altered تركيز ال plasma protein هيتغير هيزيد عندي
+
+233
+00:18:39,900 --> 00:18:44,760
+مين؟ هيزيد عندي ال .. ال acute face protein في
+
+234
+00:18:44,760 --> 00:18:47,160
+حالات ال infection و ال inflammation و ال tissue
+
+235
+00:18:47,160 --> 00:18:52,100
+injury تمام؟ اذا هاي ال acute face protein اللي
+
+236
+00:18:52,100 --> 00:18:58,020
+زادت عن حدهازادت على الحد الطبيعي، إيش هتعمل؟
+
+237
+00:18:58,020 --> 00:19:02,000
+هتعمللي reduction في ال negative charge أكتر و
+
+238
+00:19:02,000 --> 00:19:07,560
+هتعمللي تجمعات أكتر و هتقلل ال zeta potential اللي
+
+239
+00:19:07,560 --> 00:19:11,640
+ما بين الخلايا أكتر و تقرب الخلايا ما بينها و ما
+
+240
+00:19:11,640 --> 00:19:16,100
+بين بعضها أكتر، تمام؟ فبالتالي هذا إيش هيعمللي؟
+
+241
+00:19:16,100 --> 00:19:21,820
+Larger massكتلة أكبر من الخلايا تسقط أسرع، إذا أنا
+
+242
+00:19:21,820 --> 00:19:27,260
+في عندي ESR أعلى، يعني أنا ترسيب عندي في .. بيصير
+
+243
+00:19:27,260 --> 00:19:31,540
+increase in the velocity، في سرعة الترسيب، تمام؟
+
+244
+00:19:31,540 --> 00:19:34,500
+increase sedimentation velocity أو increase
+
+245
+00:19:34,500 --> 00:19:39,600
+sedimentation rate، معدل الترسيب أشماله بيصير ..
+
+246
+00:19:39,600 --> 00:19:46,490
+بيصير أعلى، تمام؟ بيصير أعلىنتيجة إنه البروتينات
+
+247
+00:19:46,490 --> 00:19:53,110
+زادت ال acute phase protein فأدت إلى تقارب أكثر ما
+
+248
+00:19:53,110 --> 00:19:57,430
+بين الخلايا و تجمعات أكتر هاي تجمعات الخلايا يا
+
+249
+00:19:57,430 --> 00:20:01,450
+بنات بسميها roll-off formation إيش بسميها؟ roll
+
+250
+00:20:01,450 --> 00:20:04,790
+-off formation ورح نشرح إيش يعني roll-off
+
+251
+00:20:04,790 --> 00:20:09,910
+formation خلال ال slides القادمة، تمام؟ بس خليني
+
+252
+00:20:09,910 --> 00:20:18,350
+أشرحلكم على هاي ال slideخلّيني أشوف خلايا متباعدة
+
+253
+00:20:18,350 --> 00:20:24,190
+عن بعضها البعض مع عملات تجمعات، تمام؟ فيعني زي
+
+254
+00:20:24,190 --> 00:20:29,490
+السيارات كأنها بتقطع مسافة أو بسرعة متباعدة مع
+
+255
+00:20:29,490 --> 00:20:35,080
+بعضها البعض، تمام؟تخيل .. تخيليها سيارات متباعدة
+
+256
+00:20:35,080 --> 00:20:40,240
+يعني هي زي ال RBCs المتباعدة، طبعا؟ فمعدل سقوط هذه
+
+257
+00:20:40,240 --> 00:20:43,960
+ال RBCs نتيجة إنها متباعدة يعني مافيش عندي ..
+
+258
+00:20:43,960 --> 00:20:50,500
+مافيش عندي تجمعات، طبعا؟ مافيش عندي تجمعات لل
+
+259
+00:20:50,500 --> 00:20:54,400
+RBCs، إذن هل هتسقط بسرعة؟ لأ، يعني هت .. ممكن تاخد
+
+260
+00:20:54,400 --> 00:20:59,540
+الوضع الطبيعي للسقوطنتيجة التباعد الطبيعي ونتيجة
+
+261
+00:20:59,540 --> 00:21:03,980
+وجود ال acute face protein بالمعدل الطبيعي تمام؟
+
+262
+00:21:03,980 --> 00:21:07,720
+زادت ال acute face protein يعني تقاربت ال RBCs
+
+263
+00:21:07,720 --> 00:21:12,280
+أكتر تمام؟ فصار في عندي ash ال .. ال .. ال .. زي
+
+264
+00:21:12,280 --> 00:21:15,500
+هنا السيارات مثلا صارت متقاربة من بعضها البعض
+
+265
+00:21:15,500 --> 00:21:19,860
+تمام؟ طيب فإذا طالما أنها تقاربت إذا صار في عندي
+
+266
+00:21:19,860 --> 00:21:25,530
+نسبة يعني ..معدل سقوطها على طريق ما، طريق يعني
+
+267
+00:21:25,530 --> 00:21:30,350
+نازل بنزول، هيكون أسرع، صح؟ تمام، نفس الفكرة هنا
+
+268
+00:21:30,350 --> 00:21:34,970
+طيب، لو كانت الخلايا متقاربة أكثر فأكثر فأكثر،
+
+269
+00:21:34,970 --> 00:21:38,610
+فتجمعت يعني، فهتكون زي ك .. زي ك .. زي كأنها قطار
+
+270
+00:21:38,610 --> 00:21:43,240
+ومسرعفي النزول و زي .. هيك عملالي تجمعات هذا
+
+271
+00:21:43,240 --> 00:21:48,340
+التجمعات أنا بسميها roll-off formation تمام؟
+
+272
+00:21:48,340 --> 00:21:51,800
+بسميها roll-off formation طب إيش يعني roll-off
+
+273
+00:21:51,800 --> 00:21:56,180
+formation؟ يعني إنه الخلايا ال RBC بتكون متجمعة
+
+274
+00:21:56,180 --> 00:22:02,240
+على بعضها البعض بشكل likestack coins إيش يعني like
+
+275
+00:22:02,240 --> 00:22:08,520
+stack coins؟ يعني زي القطع المعدنية اللي منزلقة
+
+276
+00:22:08,520 --> 00:22:13,020
+على بعضها البعض مثل القطع المعدنية اللي منزلقة على
+
+277
+00:22:13,020 --> 00:22:16,840
+بعضها البعض تخيلي أنت عندك صفة شواكل زي هيك و جيتي
+
+278
+00:22:16,840 --> 00:22:20,880
+ده حرشتيهم و منزلقوا على بعضهم البعض هذا بسميه شكل
+
+279
+00:22:20,880 --> 00:22:23,480
+ال roll-off formation نفس الشيء الخلايا بتاخد هذا
+
+280
+00:22:23,480 --> 00:22:29,020
+الشكل خليني هنا مثلا أجيأورجيكم إياه على الصور
+
+281
+00:22:29,020 --> 00:22:39,440
+لحتى تشوفوا كيف إيش يعني له low formation تمام؟
+
+282
+00:22:39,440 --> 00:22:45,690
+هنشوف الصور هناRouleau formation هاي شايفين؟ خلايا
+
+283
+00:22:45,690 --> 00:22:50,130
+ال RBCs تأخد شكل stacking coins زي كأنها قطع
+
+284
+00:22:50,130 --> 00:22:53,110
+معدنية ومنزلقة على بعض البعض زي صفتة الشواكل اللي
+
+285
+00:22:53,110 --> 00:22:58,250
+انت بتدحرجيهم تحت ال microscope بتكون بهذا الشكل
+
+286
+00:22:58,250 --> 00:23:01,790
+شايفين؟ هاي هنا كمان أيضا تحت ال microscope هذا
+
+287
+00:23:01,790 --> 00:23:05,350
+بسميه Rouleau formation هذا Rouleau formation هذا
+
+288
+00:23:05,350 --> 00:23:09,930
+Rouleau formation تمام؟ إذن هذه صورة تقريبيةو هاي
+
+289
+00:23:09,930 --> 00:23:13,750
+صورة تحت ال microscope له formation زي ال RBC's
+
+290
+00:23:13,750 --> 00:23:17,710
+منظلقة على بعض هالبعض مثل ال stack coins تمام؟
+
+291
+00:23:17,710 --> 00:23:26,100
+like stack coins طيبهيك بنكون شرحنا فكرة الفحص،
+
+292
+00:23:26,100 --> 00:23:29,580
+عرفنا إيش دور الاريثروسايت في ال .. ال
+
+293
+00:23:29,580 --> 00:23:34,980
+sedimentation، طبعا، حجمها ووجود لبروتينة ..
+
+294
+00:23:34,980 --> 00:23:39,100
+وشحناتها، ال negative charge اللي حواليها، التباعد
+
+295
+00:23:39,100 --> 00:23:45,670
+ما بينها، وجود لبروتيناتبنسبة معينة ممكن تقرب ما
+
+296
+00:23:45,670 --> 00:23:50,690
+بينها يصير في عندي تجمعات roll-off formation يعني
+
+297
+00:23:50,690 --> 00:23:55,830
+صار في عندي larger particle فهيسقط بشكل أسرع
+
+298
+00:23:55,830 --> 00:23:59,190
+وبالتالي هيصير في عندي sedimentation rate هذا
+
+299
+00:23:59,190 --> 00:24:02,670
+بالوضع الطبيعي هيكون ال sedimentation rate طبيعي
+
+300
+00:24:02,670 --> 00:24:05,630
+ونسبة أيضا البروتينات اللي ساهمت في التقارب ما بين
+
+301
+00:24:05,630 --> 00:24:09,210
+ال RBC و ال roll-off formation هتكون طبيعيةفي حالة
+
+302
+00:24:09,210 --> 00:24:11,970
+disease إذا أنا في عندي plasma protein
+
+303
+00:24:11,970 --> 00:24:15,830
+concentration عالي يعني acute face protein عالي
+
+304
+00:24:15,830 --> 00:24:19,950
+فبالتالي هيكون عندي .. هيصير في reduction في ال
+
+305
+00:24:19,950 --> 00:24:23,010
+negative charge، هيكون في تقارب ما بين ال RBCs
+
+306
+00:24:23,010 --> 00:24:27,370
+أكتر، له low formation أكتر، larger mass، increase
+
+307
+00:24:27,370 --> 00:24:31,190
+sedimentation rate، تمام؟و أيضا في حالة ال
+
+308
+00:24:31,190 --> 00:24:35,930
+agglutination يا بنات تجلط الدم او تجمع خلايا الدم
+
+309
+00:24:35,930 --> 00:24:40,930
+مع بعضها البعض و نتيجة تغير شكلها او تغير سطحها
+
+310
+00:24:40,930 --> 00:24:47,190
+هذا ايضا ممكن انه يعملي increase RBCs ماسقى يزود
+
+311
+00:24:47,190 --> 00:24:51,370
+من كتلة ال RBCs فيسرع سقوتها يعني هلأ انا طبعا لو
+
+312
+00:24:51,370 --> 00:24:57,390
+بدي ارمي يعني أسقط صخرة من علو ولا أسقط حجر صغير
+
+313
+00:24:57,390 --> 00:25:02,260
+الصخرة هتنزل أسرع نتيجة كتلتهاأعلى، تمام؟ هاي هنا
+
+314
+00:25:02,260 --> 00:25:07,160
+بس الفكرة ف ال RBCs larger particle faster rate to
+
+315
+00:25:07,160 --> 00:25:14,450
+fall طيبال .. ال macro site .. macro معناها كبير
+
+316
+00:25:14,450 --> 00:25:19,810
+حجمها كبير very .. يعني large size site خلية
+
+317
+00:25:19,810 --> 00:25:24,630
+الخلية اللي حجمها كبير tend to settle more rapidly
+
+318
+00:25:24,630 --> 00:25:29,350
+than .. mean than micro site micro يعني صغير و
+
+319
+00:25:29,350 --> 00:25:33,100
+site خلية إذا الخلايا اللي حجمها كبيرنتيجة ان ال
+
+320
+00:25:33,100 --> 00:25:36,380
+mass تبعتها كبيرة و ال size تبعتها كبير حكينا
+
+321
+00:25:36,380 --> 00:25:40,340
+اشمالها fall او settle او sedimentation بيصيرلها
+
+322
+00:25:40,340 --> 00:25:44,800
+sedimentation more rapidly اسرع من ال micro site
+
+323
+00:25:44,800 --> 00:25:51,020
+في حالة ال anisocytosis و الbuchelocytosis رح يصير
+
+324
+00:25:51,020 --> 00:25:57,480
+في عندي reduce ل ال RBCs aggregation فبالتالي هيقل
+
+325
+00:25:57,480 --> 00:26:02,980
+ال ESRإيش يعني أنيسو سايتوسز و أبو كيلو سايتوسز؟
+
+326
+00:26:02,980 --> 00:26:06,340
+هلأ الانيسو سايتوسز يعني variation في ال cell size
+
+327
+00:26:06,340 --> 00:26:12,240
+يعني تباين في حجم الخلايا، تمام؟ تباين في حجم
+
+328
+00:26:12,240 --> 00:26:16,740
+الخلايايعني الخلايا حجمها كبير، خلايا وسط، خلايا
+
+329
+00:26:16,740 --> 00:26:22,780
+صغيرة، هكذا يعني طيب بوكيلوسايتوسز تباين في شكل
+
+330
+00:26:22,780 --> 00:26:26,680
+الخلايا في shape للخلايا يعني عندي خلايا شكلها
+
+331
+00:26:26,680 --> 00:26:30,180
+الشكل الطبيعي اللي هي bacon cave او discotheque في
+
+332
+00:26:30,180 --> 00:26:34,360
+عندي خلايا spherocyte شكلها كوراوي في خلايا شكلها
+
+333
+00:26:34,360 --> 00:26:39,280
+شكل ال sickle cell من جلي، تمام؟ فهذا التباين في
+
+334
+00:26:39,280 --> 00:26:45,000
+شكل الخلايا بسميه بوكيلوسايتوسزطيب، هلأ تجمعات
+
+335
+00:26:45,000 --> 00:26:49,720
+الخلايا لازم تكون الخلايا .. يعني لحتى تحدث هاي
+
+336
+00:26:49,720 --> 00:26:52,200
+التجمعات، لحتى يحدث ال roll-off formation أو ال
+
+337
+00:26:52,200 --> 00:26:56,780
+RBC aggregation هذا لازم ان الخلايا تكون uniform،
+
+338
+00:26:56,780 --> 00:27:00,540
+منتظمة الشكل والحجم عشان انها تتجمع مع بعضها
+
+339
+00:27:00,540 --> 00:27:03,520
+البعض، تلتصق زي ال stack coins زي ما شوفنا في
+
+340
+00:27:03,520 --> 00:27:09,220
+الصورة من شوي و تسقط مع بعضها البعضكلن يعني يتجمع
+
+341
+00:27:09,220 --> 00:27:14,320
+على شكلته طبعا ال RBCs لازم تكون يعني بتشبه بعضها
+
+342
+00:27:14,320 --> 00:27:19,520
+بالشكل و الحجم علشان ايه اشملها انه يعني يصيرها
+
+343
+00:27:19,520 --> 00:27:23,300
+sedimentation مع بعض تصيرها aggregation و بعدها
+
+344
+00:27:23,300 --> 00:27:28,070
+sedimentation مع بعض احنا مثلا لما بنصاحب ناسمن
+
+345
+00:27:28,070 --> 00:27:31,610
+صاحبهم بيشبهونا مش شرط بالشكل أو بالحجم ممكن
+
+346
+00:27:31,610 --> 00:27:37,590
+يكونوا بيشبهونا باصطفات تمام؟ فإنه يعني احنا بيكون
+
+347
+00:27:37,590 --> 00:27:42,290
+في تواؤمة بينهم على نفس الفكرة ال RBCs لازم انه
+
+348
+00:27:42,290 --> 00:27:45,810
+علشان يصير لها aggregation و low formation تكون ..
+
+349
+00:27:45,810 --> 00:27:52,010
+تكون على الأقل منتظمة الشكل و الحجمطبعا طيب لكن
+
+350
+00:27:52,010 --> 00:27:55,210
+إذا كان في عندي تباين في الحجم أو تباين في الشكل
+
+351
+00:27:55,210 --> 00:28:00,630
+هذا راح يقدي إلى inhibition أو reduce the ability
+
+352
+00:28:00,630 --> 00:28:06,630
+of RBCs يقلل من قدرة خلايا ال RBCs على إنها تعمل
+
+353
+00:28:06,630 --> 00:28:12,790
+aggregation تعمل larger particle ويحلهافخلايا ال
+
+354
+00:28:12,790 --> 00:28:16,450
+RBCs راح تسقط يعني على الأغلب فرادة أو هي و هي
+
+355
+00:28:16,450 --> 00:28:22,070
+تجمعات قليلة صغيرة جدا فهذا يقلل الإيسار طبعا؟
+
+356
+00:28:22,070 --> 00:28:23,710
+معدل الترسيب
+
+357
+00:28:26,070 --> 00:28:30,630
+طيب RBCs هنا بيشرحلي إيش معناة كلمة anisocytosis
+
+358
+00:28:30,630 --> 00:28:33,650
+and bukylocytosis بتقلل ال aggregation و ال low
+
+359
+00:28:33,650 --> 00:28:37,930
+formation و بتقلل ال ESR بيحكيلك ال RBCs should
+
+360
+00:28:37,930 --> 00:28:41,730
+show an alteration in shape يعني شكلها يعني متغير
+
+361
+00:28:41,730 --> 00:28:46,600
+زي ال sickle cellخلايا المنجالية والكوراوية طبعا
+
+362
+00:28:46,600 --> 00:28:51,200
+ما .. ما .. يعني لا تتمكن أو لا تفتقد القدرة علي
+
+363
+00:28:51,200 --> 00:28:54,740
+إنها تعمل لي aggregation أو low formation فبالتالي
+
+364
+00:28:54,740 --> 00:28:58,240
+الارترو سايت sedimentation rate أشماله بيكون
+
+365
+00:28:58,240 --> 00:29:02,580
+decreased في حالة الانيسو سايتوسز والكيلو سايتوسز
+
+366
+00:29:04,000 --> 00:29:09,440
+تمام، في حالة ال severe anemia، anemia فقر دم يعني
+
+367
+00:29:09,440 --> 00:29:14,420
+في .. severe، كتير .. يعني كتير متقدمة .. متقدمة
+
+368
+00:29:14,420 --> 00:29:18,760
+الحالة، يعني كتير anemia، مستوى ال anemia كتير،
+
+369
+00:29:18,760 --> 00:29:24,120
+تمام؟ طيب، the ESR is markedly elevated، بيكون
+
+370
+00:29:24,120 --> 00:29:28,840
+عالي، ليش؟ لأن anemia يعني نقص homoglobin، يعني
+
+371
+00:29:28,840 --> 00:29:35,930
+نقص RBCsRed blood cell قليلة بالنسبة للبلازمة فلما
+
+372
+00:29:35,930 --> 00:29:40,270
+بتسقط اشملها أول حاجة مابدهاش وقت لتسقط لإن عددها
+
+373
+00:29:40,270 --> 00:29:47,330
+قليل تاني إشي لإن نسبة البلازمة بيأخد حيث أكبر من
+
+374
+00:29:47,330 --> 00:29:54,340
+الخلايا فهذارح يقدّي إلى إن ال ESR يزيد، إذا ال
+
+375
+00:29:54,340 --> 00:29:58,380
+concentration of RBCs قليل في حالة الانيميا، تركيز
+
+376
+00:29:58,380 --> 00:30:02,520
+الخلايا، عدد الخلايا قليل في ال plasma فال
+
+377
+00:30:02,520 --> 00:30:05,860
+aggregation و ال role of formation هيزيد، تمام؟
+
+378
+00:30:06,580 --> 00:30:12,060
+بالإضاءة يعني هتسقط بسرعة لإنه عددها قليل و لإنه
+
+379
+00:30:12,060 --> 00:30:16,540
+تجمعها هيكون بسرعة و لإنه نسبة البلازمة بالنسبة
+
+380
+00:30:16,540 --> 00:30:22,060
+للخلايا أعلى فهذه أسباب كافية لإنه اتخليلي ال ESR
+
+381
+00:30:22,060 --> 00:30:26,840
+markedly elevated طبعا؟ طيب في حالة ال
+
+382
+00:30:26,840 --> 00:30:33,000
+polycythemiaESR is generally decreased طبعا في
+
+383
+00:30:33,000 --> 00:30:36,860
+حالة البوليسيثيميا ال ESR is generally decreased
+
+384
+00:30:36,860 --> 00:30:41,440
+بيقل ال ESR أول إشي طبعا إحنا حكينا إنه
+
+385
+00:30:41,440 --> 00:30:46,120
+البوليسيثيميا يعني زيادة في خلايا الدم ال RBCs
+
+386
+00:30:46,120 --> 00:30:49,500
+طبعا نعكس ال concentration هنا في حالة
+
+387
+00:30:49,500 --> 00:30:54,410
+البوليسيثيميا إيش معله هيكون increaseتمام؟ يعني ال
+
+388
+00:30:54,410 --> 00:30:56,950
+aggregation و ال role of formation اشماله هيكون
+
+389
+00:30:56,950 --> 00:31:00,350
+decrease خلايا يعني بدها وقت لا تتجمع مع بعض على
+
+390
+00:31:00,350 --> 00:31:04,550
+بعض أو بدها وقت لا تسقط لإن عددها كبير تمام؟ و
+
+391
+00:31:04,550 --> 00:31:08,430
+أيضا في حق البوليسايثيميا احنا حكينا إنه نوع من
+
+392
+00:31:08,430 --> 00:31:13,410
+أنواعها أحيانا بيكون cancer تمام؟ خلية الأم لل
+
+393
+00:31:13,410 --> 00:31:17,790
+RBCs في ال bone marrow بيصير فيها mutation بيصير
+
+394
+00:31:17,790 --> 00:31:22,030
+تنقسم بشكل مجنون فبتعطيني RBCs كتير على ال
+
+395
+00:31:22,030 --> 00:31:27,060
+circulationتمام؟ في حالات ال .. ال cancer بشكل عام
+
+396
+00:31:27,060 --> 00:31:33,620
+بتزيد لزوجة ال .. البلازمة تمام؟ بتزيد ال
+
+397
+00:31:33,620 --> 00:31:40,360
+viscosity للبلازمة بتزيد لزوجة البلازمة فال RBCs
+
+398
+00:31:40,360 --> 00:31:46,960
+اللي عددها كبير high count of RBCs تمام؟ لما تيجي
+
+399
+00:31:46,960 --> 00:31:54,230
+تسقط في سائل لزج هتاخد وقت أكبر يعني هتسقط أبطأإذا
+
+400
+00:31:54,230 --> 00:32:00,450
+ال ESR إيش ماله؟ decrease تمام؟ هيكون هذا معناه ال
+
+401
+00:32:00,450 --> 00:32:03,470
+.. ال .. انه ال ESR decrease في حالة ال
+
+402
+00:32:03,470 --> 00:32:09,470
+polycythemia لو أنا جبت كورةتمام و حطيتها في tube
+
+403
+00:32:09,470 --> 00:32:14,450
+فيه ميه أسقطتها في ال tube فيه ميه و بعدين جبت كرة
+
+404
+00:32:14,450 --> 00:32:17,510
+تانية نفس الحجم و نفس الشكل و أسقطتها في ال tube
+
+405
+00:32:17,510 --> 00:32:22,150
+لنفترض مثلا فيه زيت أو glycerol أو سائل لزج مين
+
+406
+00:32:22,150 --> 00:32:26,590
+هيكون سقوط ال RBC او سقوط القرة اللي معايا أسرع
+
+407
+00:32:26,590 --> 00:32:30,110
+طبعا أكيد في ال .. في ال .. ال tube اللي فيه ميه
+
+408
+00:32:30,970 --> 00:32:35,010
+طبعا و احنا عندنا ال plasma أصلا 90% منها ماء
+
+409
+00:32:35,010 --> 00:32:41,070
+water طبعا فالمفروض أنه سقوتها أسرع في الماء لكن
+
+410
+00:32:41,070 --> 00:32:46,170
+لو زادت لزوجة ال plasma هذه فبالتالي هيصير سقوط ال
+
+411
+00:32:46,170 --> 00:32:53,130
+RBC's أبطأ هاي فكرتهاطبعا نيجي لل second player
+
+412
+00:32:53,130 --> 00:32:57,750
+اللي بيلعب دور مهم بال ESR أيضا ل plasma
+
+413
+00:32:57,750 --> 00:33:02,510
+composition the plasma composition مكونات البلازما
+
+414
+00:33:02,510 --> 00:33:06,970
+is a most important factor مهم يعني عامل لا يقل
+
+415
+00:33:06,970 --> 00:33:11,570
+أهميته عن ال RBCs في ال determining لل ESR لحتى
+
+416
+00:33:11,570 --> 00:33:17,320
+أنا أعرف قيمة ال ESRأو أقيص ال ESR رولوه و
+
+417
+00:33:17,320 --> 00:33:20,660
+Aggregation of RBCs are controlled primarily by
+
+418
+00:33:20,660 --> 00:33:23,940
+the level of acute phosphoprotein احنا شرحناها هاي
+
+419
+00:33:23,940 --> 00:33:27,880
+المعلومة حكينا انه الـRolo Formation و ال
+
+420
+00:33:27,880 --> 00:33:31,760
+Aggregation لل RBCs تقارب ال RBCs وتجمعها وتعمل
+
+421
+00:33:31,760 --> 00:33:36,920
+رولو Formation عشان تصير كتلة خلايا وتسقط أسرعطبعا
+
+422
+00:33:36,920 --> 00:33:40,340
+هذا محكم بمين؟ بال level of acute face protein
+
+423
+00:33:40,340 --> 00:33:43,600
+لنفترق .. طبعا ال acute face protein هي ماعطيني
+
+424
+00:33:43,600 --> 00:33:49,880
+مثال عليها fibrinogen, α1-globulin و α2-globulin
+
+425
+00:33:49,880 --> 00:33:54,660
+طبعا هذه كلها؟ لأ طبعا مش كلها مش ال .. مش كل ال
+
+426
+00:33:54,660 --> 00:33:57,480
+acute face protein هاي في acute face protein
+
+427
+00:33:57,480 --> 00:34:03,770
+ماذكروها ولكن هذه مثال عليهاطبعا هاي ال acute
+
+428
+00:34:03,770 --> 00:34:07,110
+facial protein حكينا إنها بتزيد في حالات tissue
+
+429
+00:34:07,110 --> 00:34:15,090
+injury, inflammation, infection طبعا طيب و بالتالي
+
+430
+00:34:15,090 --> 00:34:21,030
+إذا زادت نسبة هاي البروتينات فهتسرع طبعا إنها
+
+431
+00:34:21,030 --> 00:34:23,630
+هتعادل أول إش تقلل من ال negative charge اللي
+
+432
+00:34:23,630 --> 00:34:28,150
+موجودة حوالين ال RBCs فبالتالي هتقلل من ال
+
+433
+00:34:28,150 --> 00:34:32,860
+repulsion forcesفهتقلل من ال zeta potential يعني
+
+434
+00:34:32,860 --> 00:34:36,880
+المسافة ما بين ال RBCs هتقل و ايضا هي هتشغل
+
+435
+00:34:36,880 --> 00:34:41,120
+المسافة ما بين ال RBCs يعني هتحدث تقارب اكبر ل ال
+
+436
+00:34:41,120 --> 00:34:45,380
+RBCs يعني aggregation يعني rollout formation يعني
+
+437
+00:34:45,380 --> 00:34:50,440
+سقوط سريع لل RBCs طبعا؟ طيب خلينا بس نحكي هاي
+
+438
+00:34:50,440 --> 00:34:54,000
+الفقرة مهمة جدا يا بنات ايش .. ايش بتحكيلنا هاي
+
+439
+00:34:54,000 --> 00:35:00,680
+الفقرة؟as the concentration of a protein increases
+
+440
+00:35:00,680 --> 00:35:06,940
+كل ما زادت تركيز البروتينات في الدم أو في البلازمة
+
+441
+00:35:06,940 --> 00:35:11,680
+فبالتالي the viscosity of a plasma أيضا increase
+
+442
+00:35:11,680 --> 00:35:15,500
+طبعا as the concentration of a protein increases
+
+443
+00:35:15,500 --> 00:35:18,740
+so does the viscosity of a plasma يعني كل ما بتزيد
+
+444
+00:35:18,740 --> 00:35:22,960
+البروتينات تركيز البروتينات بيزيد لزوجة البلازمة
+
+445
+00:35:22,960 --> 00:35:29,850
+طبعاطيب فبالتالي although بالرغم من أن زيادة ال
+
+446
+00:35:29,850 --> 00:35:33,650
+plasma viscosity will tend to inhibit the fall of
+
+447
+00:35:33,650 --> 00:35:37,490
+RBCs and the increase in plasma protein are
+
+448
+00:35:37,490 --> 00:35:41,170
+generally those which cause low and aggregation of
+
+449
+00:35:41,170 --> 00:35:44,690
+RBCs which affect the ESR more greatly than the
+
+450
+00:35:44,690 --> 00:35:48,930
+increase in plasma viscosityهلأ صح إن زيادة
+
+451
+00:35:48,930 --> 00:35:54,830
+البروتينات في البلازمة ممكن إنها تزيد لزوجة
+
+452
+00:35:54,830 --> 00:36:01,250
+البلازمة، تمام؟ ولكنممكن إنها تزيد لزوجة البلازمة
+
+453
+00:36:01,250 --> 00:36:09,650
+ولكن يعني .. يعني بغض النظر إنها لو زادت لزوجة
+
+454
+00:36:09,650 --> 00:36:13,650
+البلازمة فبالتالي يعني إحنا عارفين إن إذا زادت
+
+455
+00:36:13,650 --> 00:36:17,950
+اللزوجة هي .. هي .. هي .. هيعمل إعاقة أو تبطيء
+
+456
+00:36:17,950 --> 00:36:24,200
+لسقوط خلايا الدم، تمام؟طيب، هل هذه المعلومة
+
+457
+00:36:24,200 --> 00:36:27,500
+متناقدة مع المعلومة السابقة؟ آه، هي متناقدة
+
+458
+00:36:27,500 --> 00:36:31,920
+الصراحة، انه احنا .. طب stop يعني، احنا هلأ بدنا
+
+459
+00:36:31,920 --> 00:36:35,200
+نعرف ال plasma protein أو ال acute face protein هي
+
+460
+00:36:35,200 --> 00:36:39,680
+بتزود لزوجة، فبتقلل من معدل سقوط ال RBCs، يعني
+
+461
+00:36:39,680 --> 00:36:44,180
+بتقلل ال ESR و اللي هي بتعمل role of formation و
+
+462
+00:36:44,180 --> 00:36:49,520
+aggregation و بالتالي بتزود من تجمع ال RBCs فبتسرع
+
+463
+00:36:49,520 --> 00:36:54,340
+سقوتهاشوفوا معايا، هلأ ال acute face protein بتعمل
+
+464
+00:36:54,340 --> 00:36:59,180
+الشغلتين ولكن كفة .. الكفة تبع ال roll-off
+
+465
+00:36:59,180 --> 00:37:04,780
+formation و ال aggregation بترجح أكتر، تمام؟ بتكون
+
+466
+00:37:04,780 --> 00:37:10,320
+تأثيرها أكبر، ما طالما إن هي تأثيرها في اتجاه ال
+
+467
+00:37:10,320 --> 00:37:15,160
+roll-off and aggregation of RBCs أكبر، تمام؟
+
+468
+00:37:15,160 --> 00:37:22,040
+معناها الترسيب هيكون أسرعطبعا؟ طيب و .. لكن في
+
+469
+00:37:22,040 --> 00:37:26,560
+عندي بروتين اسمه ال albumin طبعا؟ هذا البروتين
+
+470
+00:37:26,560 --> 00:37:31,360
+تقريبا هو أعلى تركيز البروتين موجود في الدم يعني
+
+471
+00:37:31,360 --> 00:37:34,920
+موجود وبيتصنع بنسبة معينة هو أعلى بروتين موجود في
+
+472
+00:37:34,920 --> 00:37:41,420
+الدم طبعا؟هذا ال protein هو اللي بيعطي الدم قوامه،
+
+473
+00:37:41,420 --> 00:37:46,720
+ان هو يعني .. يعني أكتر لزوجة قليلا من الميه،
+
+474
+00:37:46,720 --> 00:37:50,420
+تمام؟ هو اللي .. هو اللي مخلي الدم مايصيرش ميه يا
+
+475
+00:37:50,420 --> 00:37:55,560
+بنات، هاي معناه بالعربي يعني هيك ال albumin، تمام؟
+
+476
+00:37:55,560 --> 00:38:01,100
+لو زاد ال albumin هذا نتيجة مرض معين، تمام؟يعني
+
+477
+00:38:01,100 --> 00:38:06,120
+زادت لزوجة الدم هنا شايفين ال album وين بلعب؟ بلعب
+
+478
+00:38:06,120 --> 00:38:10,540
+أكتر على كفة مين؟ ع كفة اللزوجة وليس ع كفة ال roll
+
+479
+00:38:10,540 --> 00:38:14,440
+of formation and aggregation طبعا؟ فإذا ال album
+
+480
+00:38:14,440 --> 00:38:20,660
+إذا زاد بزيد اللزوجة فبقلل الإياسار طبعا؟ إذا
+
+481
+00:38:20,660 --> 00:38:26,480
+خلينا نلخص هلأ ال protein بشكل عام زيادة تركيزها
+
+482
+00:38:26,480 --> 00:38:31,470
+في الدم طبعا؟ زيادة تركيزها في الدمممكن تعملي
+
+483
+00:38:31,470 --> 00:38:34,510
+زيادة في ال roll off formation و ال aggregation
+
+484
+00:38:34,510 --> 00:38:38,370
+فبالتالي بتسرع من سقوط ال RBCs فبالتالي بتسرع من
+
+485
+00:38:38,370 --> 00:38:45,470
+ال ESR تمام increased ESR او او ممكن لبروتينات
+
+486
+00:38:45,470 --> 00:38:49,770
+high تعمل increase في ال viscosity يعني زيادة في
+
+487
+00:38:49,770 --> 00:38:55,790
+اللزوجة لبلازمة فبالتالي بتعمل إعاقة لسقوط ال RBCs
+
+488
+00:38:55,790 --> 00:39:01,690
+فبتقلل ال ESR طيبالكافتين موجودات والاحتمالين
+
+489
+00:39:01,690 --> 00:39:07,050
+موجودين، طبعا، ولكن ال acute face protein بتشتغل
+
+490
+00:39:07,050 --> 00:39:11,770
+باتجاه ال roll of formation و ال aggregation أكثر،
+
+491
+00:39:11,770 --> 00:39:14,870
+طبعا، فبالتالي عشان هيك ال acute face protein
+
+492
+00:39:14,870 --> 00:39:21,290
+بتسرع ال ESR ولكن ال albumin زيادته هيشتغل باتجاه
+
+493
+00:39:21,290 --> 00:39:26,330
+زيادة ال viscosity أكثرتمام؟ فبالتالي زيادة ال ..
+
+494
+00:39:26,330 --> 00:39:31,610
+ال album يعني increased of viscosity يعني هيعمللي
+
+495
+00:39:31,610 --> 00:39:37,150
+inhibition للfall of RBCs يعني هيعطيني lower ESR
+
+496
+00:39:37,150 --> 00:39:44,230
+أو decreased ESRتمام؟ إذاً هذا ال .. ال .. يعني
+
+497
+00:39:44,230 --> 00:39:48,370
+دور ال plasma protein في ال ESR طيب في عندي أيضًا
+
+498
+00:39:48,370 --> 00:39:52,750
+mechanical and technical factors عوامل تقنية أو
+
+499
+00:39:52,750 --> 00:40:00,010
+ميكانيكية لحتى بتأثر على قيمة ال ESR في شغلنا
+
+500
+00:40:00,010 --> 00:40:06,370
+أثناء شغلنا و هاي العوامل بتأثر على قيمة ال ESR
+
+501
+00:40:06,960 --> 00:40:10,000
+يعني كيف انا اشتغلت العينة، كيف سحبتها، كيف
+
+502
+00:40:10,000 --> 00:40:12,480
+عملتلها mixing مع ال anticoagulant، نوع ال
+
+503
+00:40:12,480 --> 00:40:16,520
+anticoagulant اللي استخدمته، هل خليت ال .. ال tube
+
+504
+00:40:16,520 --> 00:40:20,260
+vertical او مش vertical، في درجة الحرارة، هذه كلها
+
+505
+00:40:20,260 --> 00:40:23,360
+عوامل mechanical، and technical عوامل تقنية
+
+506
+00:40:23,360 --> 00:40:29,410
+وميكانيكية بتؤثر على النتيجة طبعتيمثال عليها احنا
+
+507
+00:40:29,410 --> 00:40:34,210
+حكينا ان ال ESR should be exactly perpendicular
+
+508
+00:40:34,210 --> 00:40:39,510
+عمودية تصنع درجة تسعين درجة تمام؟ تصنع .. تصنع
+
+509
+00:40:39,510 --> 00:40:44,710
+زاوية تسعين درجة مع ال bench تمام؟ فبالتالي
+
+510
+00:40:44,710 --> 00:40:49,850
+الإيمالة ال tilt .. ال tilt يعني الإيمالة الإيمالة
+
+511
+00:40:49,850 --> 00:40:54,470
+في هذه ال tube تلاتين درجة هتعطيني error تلاتين في
+
+512
+00:40:54,470 --> 00:41:00,470
+الميةطبعا ال error 30% يعني 30% زيادة في قيمة ال
+
+513
+00:41:00,470 --> 00:41:06,690
+ESR لإن إيمالة ال tube بتزود ال ESR بتزود القيمة
+
+514
+00:41:06,690 --> 00:41:11,570
+ليش طيب بتزود القيمة؟ ال tube مائل يعني عندي سطح
+
+515
+00:41:11,570 --> 00:41:17,770
+مساحة سطح في .. لا ال roll off formation أكبر يعني
+
+516
+00:41:17,770 --> 00:41:24,480
+roll off formation أكتر يعني ESR أسرع و أعلىتمام؟
+
+517
+00:41:24,480 --> 00:41:27,720
+إذا هذا هيأثر على النتائج، أنا عندي إيه أسئلة
+
+518
+00:41:27,720 --> 00:41:29,860
+عالية، يعني هي ممكن أشك أن الشخص هذا عنده
+
+519
+00:41:29,860 --> 00:41:33,660
+infection, inflammation, tissue injury، فإذا .. و
+
+520
+00:41:33,660 --> 00:41:37,460
+النتيجة ممكن تكون falsely elevated، يعني هي مرتفعة
+
+521
+00:41:37,460 --> 00:41:40,820
+بالغلط، نتيجة a tilting، نتيجة إيه معناه في
+
+522
+00:41:40,820 --> 00:41:45,870
+الاتيوب؟طيب also the rack holding the tube الراك
+
+523
+00:41:45,870 --> 00:41:49,270
+اللي انا بدأ أحط عليه القنابيب تبعتي shouldn't be
+
+524
+00:41:49,270 --> 00:41:54,910
+subject to any movement or vibration مش لازم يتعرض
+
+525
+00:41:54,910 --> 00:42:01,260
+ليه اي اهتزازات او حركة هذا ال benchتمام؟ طيب ال
+
+526
+00:42:01,260 --> 00:42:03,760
+bench اللي أنا بدي أحط عليه الركات لازم يكون ثابت
+
+527
+00:42:03,760 --> 00:42:08,780
+لأ مافيش فيه عندي vibration اهتزازات أو حركة يعني
+
+528
+00:42:08,780 --> 00:42:11,760
+مثلا أنا بدي أحط ال rack تبع القنابيب تبعي بعيد عن
+
+529
+00:42:11,760 --> 00:42:15,820
+جهاز ال centrifuge بعيد عن التلاجة بعيد عن أي جهاز
+
+530
+00:42:15,820 --> 00:42:20,400
+مشغال و ممكن يعملي ذبذبات او اهتزازات او vibration
+
+531
+00:42:20,400 --> 00:42:24,080
+ل ال bench اللي محتوط عليه القنابيب تمام؟ حتى ما
+
+532
+00:42:24,080 --> 00:42:29,090
+تهتز القنابيب فبالتالي يتأثر ال sedimentationتمام،
+
+533
+00:42:29,090 --> 00:42:33,530
+هلأ minor everyday variation in room temperature
+
+534
+00:42:33,530 --> 00:42:40,420
+don't significantly affect the ACIتغير للقليل في
+
+535
+00:42:40,420 --> 00:42:45,800
+ال ESR .. في درجة الحرارة ما بيأثر على ال ESR
+
+536
+00:42:45,800 --> 00:42:48,980
+اليوم كانت درجة الحرارة تلاتين، امبارح تسعة و
+
+537
+00:42:48,980 --> 00:42:52,960
+عشرين، بكرا واحد و تلاتين لو أنا جبت عينة من شخص و
+
+538
+00:42:52,960 --> 00:42:56,380
+اشتغلتها على التلت أيام هذه في اختلاف درجات
+
+539
+00:42:56,380 --> 00:43:01,480
+الحرارة في ال minor variation هذا ممكن يعني نتيجة
+
+540
+00:43:01,480 --> 00:43:07,400
+ال ESR تكون نفسها أو فيها تغير بسيط جداما بيأثر
+
+541
+00:43:07,400 --> 00:43:10,960
+يعني don't significantly affect ESR يعني ما بيغير
+
+542
+00:43:10,960 --> 00:43:15,380
+كتير على نتيجة ال ESR لكن large changes in
+
+543
+00:43:15,380 --> 00:43:20,120
+temperature يعني مثلا تغير من عشرين لتلاتين من
+
+544
+00:43:20,120 --> 00:43:24,140
+خمستاشر لتلاتين يعني تغير كبير في درجة الحرارة
+
+545
+00:43:24,140 --> 00:43:28,140
+فهذا ممكن يأثر على نتيجة ال ESRالـ sedimentation
+
+546
+00:43:28,140 --> 00:43:31,960
+rate increases as temperature increases كل ما زاد
+
+547
+00:43:31,960 --> 00:43:37,120
+.. كل ما زادت درجة الحرارة بتزيد أو بيزيد قيمة ال
+
+548
+00:43:37,120 --> 00:43:43,120
+ESR the length and inner diameter of ESR tube also
+
+549
+00:43:43,120 --> 00:43:47,280
+affect the final test result كمان طول و القطر
+
+550
+00:43:47,280 --> 00:43:52,370
+الداخلي لل ESR tube بيأثر على ال test resultاحنا
+
+551
+00:43:52,370 --> 00:43:57,570
+حكينا أنبيب خاصة تتذكروا حكينا أنبيب خاصة لفحص ال
+
+552
+00:43:57,570 --> 00:44:01,770
+ESR كمان شوية راح نستعرضهم، راح نشوفهم، تمام؟ وهي
+
+553
+00:44:01,770 --> 00:44:07,050
+هم موجودين هم في الخلفية طيب فال ESR tube أنبيب ال
+
+554
+00:44:07,050 --> 00:44:14,850
+ESR كل ما كانت أضيق تمام؟ يعني أرفع ال .. ال .. ال
+
+555
+00:44:14,850 --> 00:44:17,750
+bore ال internal bore .. ال bore تبعها قليل أو
+
+556
+00:44:17,750 --> 00:44:23,180
+صغيرهتعطيني lower sedimentation rate، lower ESR
+
+557
+00:44:23,180 --> 00:44:29,780
+result، ليش؟ انبوب دقيق يعني أو .. او رفيع، ضيق،
+
+558
+00:44:29,780 --> 00:44:34,320
+معناه مافيش عندي حكينا مساحة سطح كافية ل ال role
+
+559
+00:44:34,320 --> 00:44:40,300
+of formation و ال RBCs aggregation فبالتالي ال ESR
+
+560
+00:44:40,300 --> 00:44:40,780
+هيقل
+
+561
+00:44:43,650 --> 00:44:47,990
+طيب إذا فائدة significance of the test أو
+
+562
+00:44:47,990 --> 00:44:52,790
+significance of the ESR فائدة الفحص تبعي حكينا إن
+
+563
+00:44:52,790 --> 00:44:58,330
+هو بيمثل ال ESR represent a non specific response
+
+564
+00:44:58,330 --> 00:45:01,910
+to tissue damage, inflammation, infection وجود
+
+565
+00:45:01,910 --> 00:45:08,190
+disease معين وما بيأثر على أو ما بيدل أو ما بيدلل
+
+566
+00:45:08,190 --> 00:45:11,710
+على ال severity طبعته but not its severity يعني
+
+567
+00:45:11,710 --> 00:45:16,900
+مثلابيدللي على وجود infection معينة زي التهابات
+
+568
+00:45:16,900 --> 00:45:22,040
+المفاصل أو inflammation معينة سوري زي التهابات
+
+569
+00:45:22,040 --> 00:45:24,920
+المفاصل infection معينة زي ال tuberculosis مرض
+
+570
+00:45:24,920 --> 00:45:30,940
+السلطن خلل في أعضاء معينة failure في أعضاء tissue
+
+571
+00:45:30,940 --> 00:45:36,910
+injury يعني هذه كلها ممكن يدللي عليهاولكن non
+
+572
+00:45:36,910 --> 00:45:40,510
+-specific حاكينا، طبعا يعني أنا ما بعرف وين ال
+
+573
+00:45:40,510 --> 00:45:43,990
+infection هاي وين ال inflammation هاي حادثة أو ال
+
+574
+00:45:43,990 --> 00:45:48,530
+tissue injury أنا بعرف إنه فيه، بعمل detection and
+
+575
+00:45:48,530 --> 00:45:51,590
+monitoring لأ ال tissue damage و ال inflammation و
+
+576
+00:45:51,590 --> 00:45:54,790
+ال infection ولكن بيكون non-specific يعني ما بعرف
+
+577
+00:45:54,790 --> 00:45:59,170
+وينها و ما بقدر أميز ال severity طبعتهاit's
+
+578
+00:45:59,170 --> 00:46:02,610
+primarily reflected changes in plasma protein طبعا
+
+579
+00:46:02,610 --> 00:46:06,350
+هو بيتأثر أكتر إشي في ال plasma protein اللي هي
+
+580
+00:46:06,350 --> 00:46:11,310
+عبارة اللي بتكون يعني اللي بتظهر أو ممكن تزيد أو
+
+581
+00:46:11,310 --> 00:46:15,190
+يتأثر تركيزها في حالات ال acute و ال chronic
+
+582
+00:46:15,190 --> 00:46:18,590
+infection في حالات ال tumor في حالات ال
+
+583
+00:46:18,590 --> 00:46:21,710
+degenerative disease يعني الأمراض الناتجة عن organ
+
+584
+00:46:21,710 --> 00:46:25,790
+failureطبعا هذه كلها بتأثر ال plasma بروتين
+
+585
+00:46:25,790 --> 00:46:29,250
+فبالتالي هيتأثر ال ESR و ممكن يكشفلي عنها نعمل ..
+
+586
+00:46:29,250 --> 00:46:33,350
+نعمل detection لقلها و أيضا حكينا بيعملي
+
+587
+00:46:33,350 --> 00:46:38,530
+monitoring طبعا يعني follow up ل ال progress of
+
+588
+00:46:38,530 --> 00:46:43,530
+certain disease يعني ممكن يعطيني .. يعني أشوف ال
+
+589
+00:46:43,530 --> 00:46:48,860
+..يعني أراقب وضع المريض بتحسن ولا ما بتحسن؟ بتحسن
+
+590
+00:46:48,860 --> 00:46:53,940
+ولا بزيد سوء؟ تمام؟ فهذا مثلا شخص مصاب بمرض السل
+
+591
+00:46:53,940 --> 00:46:57,080
+أو بمرض الـ Rheumatoid Arthritis التهاب المفاصل
+
+592
+00:46:58,070 --> 00:47:02,650
+طبعا فممكن يعني إذا أعطينا .. يعني ال ESR عملناله
+
+593
+00:47:02,650 --> 00:47:06,650
+يا كان عالي نتيجة inflammation عملناله specific
+
+594
+00:47:06,650 --> 00:47:11,510
+test فحوصات متخصصة أكثر فكشفت عن وجود إما
+
+595
+00:47:11,510 --> 00:47:16,110
+tuberculosis يعني سل عدوى يعني infection هذه طبعا
+
+596
+00:47:16,110 --> 00:47:20,350
+بكتيريا بتسبب مرض السل طبعا أو rheumatoid
+
+597
+00:47:20,350 --> 00:47:25,140
+arthritis التهابات المفاصلة ده مرض مناعي طبعاطيب
+
+598
+00:47:25,140 --> 00:47:29,380
+فاكتشفنا في الفحوصات المتخصة انه عنده اما
+
+599
+00:47:29,380 --> 00:47:33,320
+tuberculosis او rheumatoid arthritis الطبيب هيعطل
+
+600
+00:47:33,320 --> 00:47:39,100
+المريض علاجه ممكن لحتى نشوف استجابته او نشوف حالته
+
+601
+00:47:39,100 --> 00:47:44,960
+بتتحسن او بتسوق ممكن اشتغل ال ESR مرة تانية اشوفه
+
+602
+00:47:44,960 --> 00:47:51,500
+اقارنه بالنتجة السابقة زاد او نقصلكن يعني أنا ما
+
+603
+00:47:51,500 --> 00:47:57,320
+بحدد ال severity يعني مدى سوء الحالة من أول فحص
+
+604
+00:47:57,320 --> 00:48:03,400
+طبعا ممكن بعد العلاج أشوف الفحص التاني و أقارنه
+
+605
+00:48:03,400 --> 00:48:07,780
+بالأول عشان أحكم هو اتحسن ولا ما اتحسن لكن من فحص
+
+606
+00:48:07,780 --> 00:48:11,720
+واحد لأول مرة يعني ممكن يدللي إنه في infection أو
+
+607
+00:48:11,720 --> 00:48:17,360
+inflammationهو المريض وضعه تعبان على الآخر ولا في
+
+608
+00:48:17,360 --> 00:48:23,180
+بدايات المرض ممكن نلحج ولا لأ هذه أمور لا يمكن ان
+
+609
+00:48:23,180 --> 00:48:26,660
+احنا نعرفها من خلال ال ESR اللي هو فحص لحاله يعني
+
+610
+00:48:26,660 --> 00:48:32,500
+او انه نتيجة واحدة او حتى فحص لحاله بده other test
+
+611
+00:48:32,500 --> 00:48:35,100
+طيب
+
+612
+00:48:36,180 --> 00:48:41,380
+an elevated ESR may be found in زيادة ال ESR ممكن
+
+613
+00:48:41,380 --> 00:48:45,220
+تكون موجودة في حالات هلأ بتشوفوا الحالات هذه هتكون
+
+614
+00:48:45,220 --> 00:48:47,920
+إما عبارة عن inflammation أو infection أو tissue
+
+615
+00:48:47,920 --> 00:48:51,940
+injury خلينا نشوف acute and chronic infection
+
+616
+00:48:51,940 --> 00:48:55,120
+حالات ال acute و ال chronic infection في حالات ال
+
+617
+00:48:55,120 --> 00:48:58,460
+rheumatic fever اللي هو الحمه ال rheumatoid في
+
+618
+00:48:58,460 --> 00:49:03,680
+حالات ال rheumatoid arthritis التهيبات المفاصل
+
+619
+00:49:04,370 --> 00:49:08,530
+myocardial infection اللي هو عدوى او infection في
+
+620
+00:49:08,530 --> 00:49:13,330
+عضلة القلب nephrosis يعني infection في ال kidney
+
+621
+00:49:13,330 --> 00:49:18,190
+في ال nephrons acute hepatitis يعني التهابات في ال
+
+622
+00:49:18,190 --> 00:49:22,650
+liver menstruation ال period طب ما هي ال period
+
+623
+00:49:22,650 --> 00:49:26,410
+اصلا عبارة عن سقوط بطانة الرحم يعني tissue injury
+
+624
+00:49:26,410 --> 00:49:31,160
+صار طبعا فطبيعي يزيد ال ESRTuberculosis مراد السل،
+
+625
+00:49:31,160 --> 00:49:34,840
+infection high وأمراض الغضب الدراقية سواء كان
+
+626
+00:49:34,840 --> 00:49:39,180
+hypothyroidism يعني نقص في هرمونات الغضب الدراقية
+
+627
+00:49:39,180 --> 00:49:42,920
+أو hyperthyroidism يعني زيادة، hyp نقصان وhyper
+
+628
+00:49:42,920 --> 00:49:48,100
+زيادةالـ Pregnancy بعد الشهر التالت أيضًا بيزيد
+
+629
+00:49:48,100 --> 00:49:51,400
+فيها ال ESR بس مش بسبب وجود infection أو
+
+630
+00:49:51,400 --> 00:49:55,000
+inflammation أو tissue injury لأ انه في حق ال ..
+
+631
+00:49:55,000 --> 00:50:00,440
+ال pregnancy في الوضع الطبيعي بتزيد عندها بعد ش ..
+
+632
+00:50:00,440 --> 00:50:04,200
+ست الحامل بعد الشهر التالت بتزيد عندها نسبة ال
+
+633
+00:50:04,200 --> 00:50:06,900
+acute facial protein بيزيد عندها ال fibrinogen
+
+634
+00:50:06,900 --> 00:50:11,160
+طبعًا فطبيعي أنه ال ESR يزيد مش بسبب حالة مرضية
+
+635
+00:50:11,160 --> 00:50:15,500
+إما بحالة مؤقتة نتيجة وجود الحامل يعنيتمام؟ الشخص
+
+636
+00:50:15,500 --> 00:50:20,540
+اللي .. اللي عمره أكتر من ستين سنة تجاوز الستين
+
+637
+00:50:20,540 --> 00:50:25,200
+سنة ممكن ألاقي عنده slightly higher ESR يعني ESR
+
+638
+00:50:25,200 --> 00:50:30,380
+عالي نسبيا قليلا نتيجة إيش؟ أنه جسمه بشكل طبيعي
+
+639
+00:50:30,380 --> 00:50:35,180
+بلش ينتج album أقل من الطبيعي يعني أقل من ما هو
+
+640
+00:50:35,180 --> 00:50:41,230
+معتاد عليه في سن الشباب يعنيتمام؟ فإذا إجاني شخص
+
+641
+00:50:41,230 --> 00:50:46,610
+أكتر من ستين سنة وعملتله ESR ولاجئته عالي مش شرط
+
+642
+00:50:46,610 --> 00:50:49,670
+يكون عنده inflammation, infection or tissue injury
+
+643
+00:50:49,670 --> 00:50:55,110
+ممكن نتيجة السن أن هو جسمه بعد العمر هذا كله بطل
+
+644
+00:50:55,110 --> 00:50:58,930
+انه .. او يعني توقف عن الصناع المزيد او يعني كميات
+
+645
+00:50:58,930 --> 00:51:02,130
+كبيرة من ال album قلت نسبة ال album اللي بصنعها
+
+646
+00:51:02,130 --> 00:51:07,370
+يعني قلت لزوجة الدمإذا زاد ال ESR زاد الترسيب زاد
+
+647
+00:51:07,370 --> 00:51:14,150
+معدل سقوط ال RBCs طيب الأسباب التالية بت .. بت ..
+
+648
+00:51:14,150 --> 00:51:22,460
+يعني هي مسئولة عن decreased ESRdecreased ESR نقصان
+
+649
+00:51:22,460 --> 00:51:27,440
+ال ESR أو يعني قيمة قليلة لل ESR will be present
+
+650
+00:51:27,440 --> 00:51:31,720
+in هنشوفها وين في حالات ال polycythemia ذكرنا ليش؟
+
+651
+00:51:31,720 --> 00:51:35,820
+عندي RBCs كتيرة، بدها وقت لتسقط، role of formation
+
+652
+00:51:35,820 --> 00:51:40,480
+and aggregation قليل، viscosity عاليةفخصوصا لو
+
+653
+00:51:40,480 --> 00:51:44,040
+كانت حالتي ال polycythemia هذه سببها ورم فبالتالي
+
+654
+00:51:44,040 --> 00:51:49,200
+هيكون عندى ال ESR قليل ال RBCs بدها وقت أطول لتسقط
+
+655
+00:51:49,200 --> 00:51:54,940
+يعني المعدل سقوطها اشماله عالي معدل سقوطها قليل
+
+656
+00:51:54,940 --> 00:51:59,620
+سرعتها قليلة طبعا انا بحكي عن معدل سقوط يعني اشمال
+
+657
+00:51:59,620 --> 00:52:04,120
+.. يعني سرعة سرعتها قليلة فال ESR decreased في هذه
+
+658
+00:52:04,120 --> 00:52:09,160
+الحالة decreasedcongestive heart failure طبعا يعني
+
+659
+00:52:09,160 --> 00:52:14,940
+ف .. فشل في أي عضو من الأعضاء يعني أو في .. في
+
+660
+00:52:14,940 --> 00:52:20,860
+القلب تحديدا هذا هيعملي decreased ESR طيب ليش؟
+
+661
+00:52:20,860 --> 00:52:24,620
+congestive heart failure فشل في القلب يعني مافيش
+
+662
+00:52:24,620 --> 00:52:29,240
+ضخ دم كافيطبعا؟ يعني فيش oxygen delivery كافي،
+
+663
+00:52:29,240 --> 00:52:33,480
+طبعا؟ يعني في عندي hypoxia، نقص في ال oxygen، وين؟
+
+664
+00:52:33,480 --> 00:52:37,540
+في ال tissue و في ال organ، في جسم الإنسانطيب احنا
+
+665
+00:52:37,540 --> 00:52:42,060
+حكينا انه البون مارقش ماله بيشتغله مغمض عناق بس
+
+666
+00:52:42,060 --> 00:52:47,320
+بتحسس ال oxygen و اذا كان ناقص بيبدأ يصنع خلايا
+
+667
+00:52:47,320 --> 00:52:52,580
+بدون ما يفهم وين ال .. وين الخلل تمام؟ طيب بدون ما
+
+668
+00:52:52,580 --> 00:52:56,900
+يشوف انه المشكلة في القلب مش في التصنيع فبيبدأ
+
+669
+00:52:56,900 --> 00:53:02,420
+يصنع ايش RBC's كتيرةتطلع RBCs زيادة على ال
+
+670
+00:53:02,420 --> 00:53:05,420
+circulation إذا أصبحت الحالة بتشبه ال polycythemia
+
+671
+00:53:05,420 --> 00:53:10,500
+يعني زادت ال RBCs يعني بدها وقت أطول لإنه تترسم
+
+672
+00:53:10,500 --> 00:53:15,020
+يعني سرعتها هتكون بطيقة هذا معناه إنه بدها وقت
+
+673
+00:53:15,020 --> 00:53:20,560
+أطول لتترسم hypofibrogenemia يعني نقصان الفيبروجين
+
+674
+00:53:20,560 --> 00:53:26,470
+نقصان أحد ال acute face protein تمام؟نقصان الـ
+
+675
+00:53:26,470 --> 00:53:30,810
+fibrinogen اللي هو نقصان أحد ال .. ال acute face
+
+676
+00:53:30,810 --> 00:53:35,210
+protein بشكل طبيعي يعني الجسم ما بيصنع fibrinogen
+
+677
+00:53:35,210 --> 00:53:39,230
+أقل من الطبيعي فبالتالي برضه كمان هذا هيعمل لي
+
+678
+00:53:39,230 --> 00:53:44,310
+inhibition لل aggregation و ال raw formation of
+
+679
+00:53:44,310 --> 00:53:50,110
+RBCs فبالتالي هيكون في عندى ال ESR will decrease
+
+680
+00:53:50,750 --> 00:53:54,270
+كمان حالات الـ bukylocytosis و ال anisocytosis و
+
+681
+00:53:54,270 --> 00:53:58,730
+وجود .. وجود spherocytes و sickle cell في ال .. مع
+
+682
+00:53:58,730 --> 00:54:03,070
+خلايا أيضا يعني تباين في الحجم و الشكل هذا هيعمل
+
+683
+00:54:03,070 --> 00:54:05,330
+ليه inhibition لال roll off formation اذا
+
+684
+00:54:05,330 --> 00:54:12,270
+decreased ESR تمام؟طيب خلينا نشوف كيف احنا بنشتغل
+
+685
+00:54:12,270 --> 00:54:16,970
+فحص ال ESR طبعا في عندي نوعين او طريقتين انا بشتغل
+
+686
+00:54:16,970 --> 00:54:20,730
+فيهم حسب نوع ال tube اللي بشتغل فيها نوعين من ال
+
+687
+00:54:20,730 --> 00:54:25,750
+tubes وطريقتين طبعا ويستيرجرين method بستخدم فيها
+
+688
+00:54:25,750 --> 00:54:30,370
+أنابيب اسمها wystergren tube طبعا وفي عندي وينتروب
+
+689
+00:54:30,370 --> 00:54:34,950
+method بستخدم أنابيب اسمها وينتروب tube طبعا طيب
+
+690
+00:54:34,950 --> 00:54:41,630
+خلينا نشوف ال ال ..الوستيجرين method بيحكيلي انا
+
+691
+00:54:41,630 --> 00:54:46,110
+ممكن استخدم عينة عينة طبعا هو ال blood طبيعي اكيد
+
+692
+00:54:46,110 --> 00:54:50,950
+يعني الدم كامل بدون فصل انا باخده و طبعا اذا بدي
+
+693
+00:54:50,950 --> 00:54:54,670
+اخد مثلا اربع امقال لازم اضيفه على one امقال tube
+
+694
+00:54:54,670 --> 00:54:58,150
+فيها او على .. على tube فيها one امقال sodium
+
+695
+00:54:58,150 --> 00:55:03,460
+citrateطبعا؟ يعني النسبة أربعة لواحد ال dilution
+
+696
+00:55:03,460 --> 00:55:08,920
+هيكون كام؟ هيكون واحد لخمسة مين اللي مخفف؟ ال
+
+697
+00:55:08,920 --> 00:55:13,640
+citrate والله اللي مخفف لإنه اللي هو الكمية الأقل
+
+698
+00:55:13,640 --> 00:55:18,840
+صح؟ واحد لخمسة لما أحكي dilution واحد لخمسة طبعا؟
+
+699
+00:55:18,840 --> 00:55:23,800
+يعني انه .. إذا بدحكي خمسة لواحد بحكي عن ال blood
+
+700
+00:55:24,830 --> 00:55:28,970
+طبعا احنا اخدنا قوانين ال dilution فبإمكانكم انكم
+
+701
+00:55:28,970 --> 00:55:35,670
+تحسبوها و تعرفوا كم ال dilution طبعا اذا انا
+
+702
+00:55:35,670 --> 00:55:40,310
+استخدمت edita anticoagulated blood يعني ال blood
+
+703
+00:55:40,310 --> 00:55:43,490
+انا ماحطيته في تيوب فيه بعد ما جمعته ماحطيته في
+
+704
+00:55:43,490 --> 00:55:47,070
+تيوب فيها citrate انما حطيته في تيوب فيها edita
+
+705
+00:55:47,070 --> 00:55:53,470
+طبعاطبعا ف بيحكيلي انه هاي ال editor قبل ما انا
+
+706
+00:55:53,470 --> 00:55:59,690
+املأ .. املأ العينة بال Westigren tube لازم كل
+
+707
+00:55:59,690 --> 00:56:02,570
+اتنين امقال من ال blood ال anticoagulated بال
+
+708
+00:56:02,570 --> 00:56:09,530
+editor احطلهم نص امقال من محلول ملح فيه تركيز
+
+709
+00:56:09,530 --> 00:56:12,450
+point تمانية خمسة في المية sodium chloride اللي هو
+
+710
+00:56:12,450 --> 00:56:18,280
+ال salineتمام؟ أربع أمقال بدي أحطولهم واحد أمقال
+
+711
+00:56:18,280 --> 00:56:22,980
+sodium chloride solution اللي هو إيش بنسميه؟ بسميه
+
+712
+00:56:22,980 --> 00:56:26,600
+saline اللي هو المحلول الملح اللي إحنا ممكن نشتريه
+
+713
+00:56:26,600 --> 00:56:33,310
+من ال .. من السيدلياتهو المحلول الملحي أيضًا اللي
+
+714
+00:56:33,310 --> 00:56:39,270
+إحنا ممكن نحطه بالوريد للناس اللي بحتاجوا تخدية
+
+715
+00:56:39,270 --> 00:56:45,170
+وريدية فبالتالي هذا هو المحلول الملحي مصمم بتركيز
+
+716
+00:56:45,170 --> 00:56:50,410
+0.85% Sodium Chloride لحتى يحافظ على شكل الخلية
+
+717
+00:56:50,410 --> 00:56:55,630
+على الـ Osmotic Pressure تبع الخلية، تمام؟فيعني
+
+718
+00:56:55,630 --> 00:56:59,830
+بيستخدم لحتى أنا بضيفه على ال Edeta Tube طب ليش
+
+719
+00:56:59,830 --> 00:57:02,070
+بضيفه على ال Edeta لإنه ال Edeta مع ال Sodium
+
+720
+00:57:02,070 --> 00:57:05,410
+Chloride بيعطيني نفس ال Osmotic Pressure زي ما
+
+721
+00:57:05,410 --> 00:57:09,550
+أستخدم Sodium Citrate لحاله طبعا، إذا يا أما أربع
+
+722
+00:57:09,550 --> 00:57:14,190
+ML من ال Holy Blood بضيفهم على Tube فيها 1 ML من
+
+723
+00:57:14,190 --> 00:57:20,220
+Sodium Citrate أو أربع ML من ال Edeta بضيفلهمone
+
+724
+00:57:20,220 --> 00:57:26,100
+ml من ال .. ال sodium chloride solution تمام؟ و
+
+725
+00:57:26,100 --> 00:57:31,080
+بعد هيك ب .. يعني بعبي لل .. ال .. وستيجرين اتوم
+
+726
+00:57:31,080 --> 00:57:41,200
+تمام؟طيب ليش أنا ضفت على ال Edita .. يعني ليش ضفت
+
+727
+00:57:41,200 --> 00:57:44,320
+على ال Edita saline؟ أول إشي .. أول سبب علشان
+
+728
+00:57:44,320 --> 00:57:49,160
+أحافظ على ال osmolality تمام؟ و تانيش إشي يا بنات
+
+729
+00:57:49,160 --> 00:57:53,900
+عشان .. عامل ال dilution هلأ ال sodium citrate ..
+
+730
+00:57:53,900 --> 00:57:56,900
+ال sodium citrate لما أنا أحط one ml volume هذا
+
+731
+00:57:56,900 --> 00:58:02,480
+تمام؟ هذا حجمالستريد سائل و أنا حطيت على الأربعة
+
+732
+00:58:02,480 --> 00:58:09,260
+أمقال ل blood one أمقال من هذا الستريد طيب لكن ال
+
+733
+00:58:09,260 --> 00:58:15,120
+Edita بتكون يا بنات powder وجزء لا يكاد يذكر يكون
+
+734
+00:58:15,120 --> 00:58:19,060
+ممسوح في .. في .. في تيوب ال Edita فلما أجي أنا
+
+735
+00:58:19,060 --> 00:58:24,610
+أضيف أربعة أمقال على تيوب ال Edita ما بتغيروشأو
+
+736
+00:58:24,610 --> 00:58:27,790
+بيزيدوا بنسبة ضئيلة جدا، يعني بيضلوا أربعة زي ما
+
+737
+00:58:27,790 --> 00:58:30,990
+هما، هان أربعة أنا ضيفت عليها one أم ال من ال
+
+738
+00:58:30,990 --> 00:58:33,330
+secret يعني صاروا ال volume خمسة ال total volume
+
+739
+00:58:33,330 --> 00:58:37,190
+أما هنا ال Edita مع ال blood لما أنا أضيف ال blood
+
+740
+00:58:37,190 --> 00:58:41,030
+على ال tube فيها Edita، بيضال أربعة زي ما هو، لإن
+
+741
+00:58:41,030 --> 00:58:46,050
+ال Edita بودر وي بتكون يدوب يعني على الجدار ال
+
+742
+00:58:46,050 --> 00:58:51,330
+tube، كمية لات كانت تذكرفانا لازم اضيف ال one ml
+
+743
+00:58:51,330 --> 00:58:57,290
+solution من ال saline solution علشان احافظ على نفس
+
+744
+00:58:57,290 --> 00:59:01,150
+النسب نفس ال dilution نفس ال proportion انا اخدت
+
+745
+00:59:01,150 --> 00:59:06,130
+تمام؟ اتمنى انها تكون واضحة و بعد ما انا املأ
+
+746
+00:59:06,130 --> 00:59:09,750
+الwistigrine pipe او الwistigrine tube بخليها
+
+747
+00:59:09,750 --> 00:59:16,410
+allow it stand بخليها انها تكونو يعني بال vertical
+
+748
+00:59:16,410 --> 00:59:21,610
+position لمدة one hour و بعد هيك أنا بقيس المسافة
+
+749
+00:59:21,610 --> 00:59:24,430
+اللي تقطعها ال RBC بال millimeter per one hour
+
+750
+00:59:24,430 --> 00:59:30,050
+المعدد الطبيعي للنساء من 0 ل 15 عند الرجال من 0 ل
+
+751
+00:59:30,050 --> 00:59:33,810
+10 أو عند الأطفال أيضا من 0 ل 10 millimeter per
+
+752
+00:59:33,810 --> 00:59:39,870
+hourطبعا عند النساء أكتر لإنه النساء يعني نسبة ال
+
+753
+00:59:39,870 --> 00:59:43,870
+plasma protein عندهم مختلفة تمام؟ بعدين النساء
+
+754
+00:59:43,870 --> 00:59:48,230
+بتكون يعني إما طالعة من .. من period أو فايتة على
+
+755
+00:59:48,230 --> 00:59:51,730
+period أو يعني .. يعني فال plasma protein و ال
+
+756
+00:59:51,730 --> 00:59:55,350
+tissue injury و الحلجات هادى يعني بتكون .. بتأثر و
+
+757
+00:59:55,350 --> 00:59:59,270
+.. فبالتالي هي نسبتها بتكون أعلى بشكل طبيعي يعني
+
+758
+00:59:59,270 --> 01:00:00,090
+هاد بتكون أعلى
+
+759
+01:00:03,160 --> 01:00:10,460
+طمام؟ إذا هذا هو ال normal ranges طيب
+
+760
+01:00:10,460 --> 01:00:14,340
+إيش محتاجة؟ محتاجة للتجربة تبعتي عينة ال blood
+
+761
+01:00:14,340 --> 01:00:17,720
+اللي أنا بدي أسحبها و أحطها في ال .. في ال .. إما
+
+762
+01:00:17,720 --> 01:00:21,940
+في سترات أو في إيدي تتيوب لو حطيتها في سترات خلاص
+
+763
+01:00:21,940 --> 01:00:26,390
+يعني هي 4 ml blood على 1 ml sodium citrateو إذا
+
+764
+01:00:26,390 --> 01:00:29,110
+حطيتها في إيدي تتيوب لازم أضيف one and a half من
+
+765
+01:00:29,110 --> 01:00:32,590
+ال saline عشان بالنهاية أنا بدي نفس النسبة و نفس
+
+766
+01:00:32,590 --> 01:00:35,810
+ال dilution و نفس ال proportion و نفس ال is
+
+767
+01:00:35,810 --> 01:00:39,550
+molality تمام؟ بعدين بملأ ال westigran tube زي ما
+
+768
+01:00:39,550 --> 01:00:42,830
+أنتوا شايفين ال westigran tube بهذا الشكل و لازم
+
+769
+01:00:42,830 --> 01:00:47,110
+يكون إلها أبعاد طبعا إحنا حكينا انبوب خاصة يعني
+
+770
+01:00:47,110 --> 01:00:50,430
+ماقاش معنا specific tube يعني إنها لازم تكون
+
+771
+01:00:50,430 --> 01:00:56,960
+أبعادها خاصة أو standardالطول تبعها ال external
+
+772
+01:00:56,960 --> 01:01:00,940
+diameter القطر الخارجي القطر الداخلي عليها تدريج
+
+773
+01:01:00,940 --> 01:01:07,690
+معين هذا كله لازم يكون يعني ضمن معاييرو أيضا بحتاج
+
+774
+01:01:07,690 --> 01:01:12,790
+ال rack لهاي ال .. القنابيب هذا اللي لونه أزرق هو
+
+775
+01:01:12,790 --> 01:01:17,430
+اللي بسميه rack، تمام؟ و طبعا كل ال rackات لازم
+
+776
+01:01:17,430 --> 01:01:21,590
+تكون فيها حاجة زي ال bulb بتأكدلي أن هذا ال rack
+
+777
+01:01:21,590 --> 01:01:26,850
+يعني جالس بشكل طبيعي أو بشكل يعني كويس على ال
+
+778
+01:01:26,850 --> 01:01:31,730
+bench فهو عمودي، مية بالمية عمودي، تمام؟
+
+779
+01:01:34,970 --> 01:01:39,670
+يعني كيف هيكون فيها bulb خليني بس أذكرلكم مثال
+
+780
+01:01:39,670 --> 01:01:44,270
+شوفتوا عامل البناء اللي بيكون بيبني حيطان أو إشي
+
+781
+01:01:44,270 --> 01:01:47,970
+بيكون معاه زي مصطرة حديدية بيسميها ميزان وهي
+
+782
+01:01:47,970 --> 01:01:52,110
+المصطرة الحديدة في نصها في نصها في انبوبة زجاجية
+
+783
+01:01:52,110 --> 01:01:56,270
+فيها سائل أصفرطبعا هذه العامل البناء بيبني الحائط
+
+784
+01:01:56,270 --> 01:02:00,130
+بعد هيك او يعني لما بيجي بده يبني بيحط ال .. مصدر
+
+785
+01:02:00,130 --> 01:02:05,930
+الحائط طبعته و بيشوف مدى استيواق او اعتدال الحائط
+
+786
+01:02:06,660 --> 01:02:11,260
+لو كان في عندي خلل في مستوى الماء يعني في إيمالة
+
+787
+01:02:11,260 --> 01:02:15,660
+فيه معناه هو الله الاشي مش عمودي أو مش مستوي و هنا
+
+788
+01:02:15,660 --> 01:02:18,160
+نفس الفكرة يعني لازم يكون في عندي bulb لازم يكون
+
+789
+01:02:18,160 --> 01:02:22,020
+في عندي علامة من خلالها أعرف إن هذا ال .. الراك
+
+790
+01:02:22,020 --> 01:02:27,010
+جالس بالشكل ال vertical أو لاو أيضا كمان لازم يكون
+
+791
+01:02:27,010 --> 01:02:33,650
+في عندي timer لحتى يعني أضبطه على one hour و
+
+792
+01:02:33,650 --> 01:02:37,890
+يحسبلي ساعة و ي run بعد نهاية الساعة عشان أجي أقرأ
+
+793
+01:02:37,890 --> 01:02:45,400
+النتائجطيب إذا أنا إيش محتاجة؟ محتاجة إما عينة
+
+794
+01:02:45,400 --> 01:02:52,380
+blood أربع أمقال في سترية one ml أو أحط أربع أمقال
+
+795
+01:02:52,380 --> 01:02:59,580
+من ال Edita و أحط عليهم one ml من ال .. ال saline
+
+796
+01:02:59,580 --> 01:03:03,760
+أو كل اتنين أمقال أضيفلهم نص من ال sodium chloride
+
+797
+01:03:03,760 --> 01:03:08,220
+solution اللي هو ال saline، تمام؟هي في عندكم point
+
+798
+01:03:08,220 --> 01:03:12,420
+تمانية خمسة في المية wet per volume عندكم بمحاضرة
+
+799
+01:03:12,420 --> 01:03:16,100
+للمناقش ان شاء الله هسألكم كيف حضرناها هذه عشان
+
+800
+01:03:16,100 --> 01:03:19,120
+تعرفولي ال wet per volume احنا كيف حضرنا محلول ال
+
+801
+01:03:19,120 --> 01:03:19,500
+saline
+
+802
+01:03:22,320 --> 01:03:25,720
+طبعا التجربة مش رح أشرحها لإنها شبه صارت واضحة و
+
+803
+01:03:25,720 --> 01:03:30,940
+لإنه إحنا راح نشتغل بالمعامل بطريقة مختلفة طبعا لا
+
+804
+01:03:30,940 --> 01:03:35,040
+هي Winthrop ولا Westgren طريقة مختلفة فخلينا ..
+
+805
+01:03:35,040 --> 01:03:37,520
+يعني أنتوا هيكصرتوا أصلا already فاهمين يعني إيش
+
+806
+01:03:37,520 --> 01:03:44,910
+التجربة أو كيف بتنشغل ولكن يعني خلونا ..نختصر
+
+807
+01:03:44,910 --> 01:03:52,430
+موضوع ال procedure Wintrop and Landsberg method او
+
+808
+01:03:52,430 --> 01:03:57,580
+اختصارا بنحكي عنها Wintrop methodتمام؟ الفكرة هنا
+
+809
+01:03:57,580 --> 01:04:02,000
+أنه أنا ممكن أستخدم Edita Anti-coagulated blood
+
+810
+01:04:02,000 --> 01:04:05,940
+تمام؟ و على طول بس هك أحط و أعبيه في tube اسمها
+
+811
+01:04:05,940 --> 01:04:11,620
+windrop tube و أجلسها لمدة one hour شك على الوضع
+
+812
+01:04:11,620 --> 01:04:15,900
+ال vertical و بعد هك أقرأ النتائج فعندي أنه النساء
+
+813
+01:04:15,900 --> 01:04:23,160
+المعدل الطبيعي من 0 ل 20 و الرجال
+
+814
+01:04:23,160 --> 01:04:31,090
+من 0 ل 9طبعا فهذه هي ال .. ال normal ranges ل ال
+
+815
+01:04:31,090 --> 01:04:33,930
+windrop tube إيش محتاجة؟ محتاجة أتيوبات windrop
+
+816
+01:04:33,930 --> 01:04:37,890
+محتاجة ال rack تبعهم محتاجة .. يعني ممكن أحتاج
+
+817
+01:04:37,890 --> 01:04:44,710
+capillary pipe أو يعني محتاجة a sticker يسكر طرف
+
+818
+01:04:44,710 --> 01:04:49,030
+الإنبوبة من جهة عشان أثبتها على ال rack بعد هيك و
+
+819
+01:04:49,030 --> 01:04:54,190
+محتاجة whole blood طبعاone ml using edita
+
+820
+01:04:54,190 --> 01:05:00,730
+anticoagulant نفس الفكرة عينة plot مجموعة في edita
+
+821
+01:05:00,730 --> 01:05:05,790
+tube بعبي منها ال capillary pipet أو يعني بعبي
+
+822
+01:05:05,790 --> 01:05:07,890
+منها باستخدام ال capillary pipet اللي هي ال wind
+
+823
+01:05:07,890 --> 01:05:13,650
+turbine tube و بحط بسكرها بانطرفها و بعد هيك بحطها
+
+824
+01:05:13,650 --> 01:05:17,990
+على ال rack بال vertical position لمدة ساعة و بقرأ
+
+825
+01:05:17,990 --> 01:05:22,360
+هاي هنا أهل ال procedure تبعتيطبعا خلينا بس نستعرض
+
+826
+01:05:22,360 --> 01:05:29,860
+كيف شكل ال .. ال .. ال wind drop tube او sorry هاي
+
+827
+01:05:29,860 --> 01:05:34,840
+ال westigran هاي westigran tube مع ال .. ال sodium
+
+828
+01:05:34,840 --> 01:05:37,780
+citrate tube اللي بيكون فيها one ml sodium citrate
+
+829
+01:05:37,780 --> 01:05:42,560
+و هذه هي ال ESR tube مدرجة طبعا انا بفوتها و بيكون
+
+830
+01:05:42,560 --> 01:05:47,620
+نهاية التدريج عند zero طبعا فالمنطقة ال zero بتكون
+
+831
+01:05:47,620 --> 01:05:49,040
+في الأعلى طبعا
+
+832
+01:05:55,410 --> 01:06:00,950
+هي .. ها دي .. بحكي عنها winter بتيوب، هي أسمك
+
+833
+01:06:00,950 --> 01:06:07,230
+شوية، تمام؟
+
+834
+01:06:07,230 --> 01:06:16,750
+تمام
+
+835
+01:06:20,360 --> 01:06:27,220
+طيب نيجي لمناقشة بعض النقاط المتعلقة بالفحص ال ESR
+
+836
+01:06:27,220 --> 01:06:34,440
+أول إشي أثناء ترسيب خلايا ال RBCs تمر عملية
+
+837
+01:06:34,440 --> 01:06:39,090
+الترسيب بثلاث مراحلالمرحلة الأولى اللى هى خلال أول
+
+838
+01:06:39,090 --> 01:06:42,650
+عشر دقائق فى فحص الترسيب خلال أول عشر دقائق فى
+
+839
+01:06:42,650 --> 01:06:46,710
+الساعة بسميها ال lag phase و فى هذا .. فى هذا
+
+840
+01:06:46,710 --> 01:06:50,190
+المرحلة بيكون الخلايا بس عم تعمل roll off
+
+841
+01:06:50,190 --> 01:06:53,870
+formation تمام؟ ال sedimentation بيكون قليل slight
+
+842
+01:06:53,870 --> 01:06:58,960
+قليلطبعا طيب بعد في الاربع .. في الاربعين دقيقة
+
+843
+01:06:58,960 --> 01:07:04,340
+اللتي تليها بيبدأ او بيكون في طور او مرحلة اسمها
+
+844
+01:07:04,340 --> 01:07:07,280
+ال decantation phase او ال sedimentation phase
+
+845
+01:07:07,280 --> 01:07:10,640
+اللي بيكون فيها عملية ال sedimentation اشمالها
+
+846
+01:07:10,640 --> 01:07:15,940
+سريعة rapid and constant سريعة ومعدل ثابت للترسيب
+
+847
+01:07:16,460 --> 01:07:21,360
+طيب في آخر عشر دقائق بيحدث ال stationary phase ان
+
+848
+01:07:21,360 --> 01:07:27,280
+هو يعني بس مجرد تراكم و تفنيش لالترسيب يعني انه هو
+
+849
+01:07:27,280 --> 01:07:31,020
+finalization بس فقط لغير انه بيكون في عندي
+
+850
+01:07:31,020 --> 01:07:36,320
+accumulation of RBCs صار في تراكم لل RBCs في قاع
+
+851
+01:07:36,320 --> 01:07:39,500
+ال tube و صار في عندي ان ال sedimentation rate
+
+852
+01:07:39,500 --> 01:07:46,230
+بيكون slow و ..طبعا كل ما كان ال tube أطول كل ما
+
+853
+01:07:46,230 --> 01:07:49,350
+كان طول في هذا ال face بيكون .. يعني بياخد وقت
+
+854
+01:07:49,350 --> 01:07:53,890
+أطول هذا ال face طبعا هذا إذا ال stationary face
+
+855
+01:07:53,890 --> 01:08:00,010
+طيب إذا بتسأله عنهم إيش اسم ال face و ماذا يحدث
+
+856
+01:08:00,010 --> 01:08:05,450
+فيه طبعا ممكن أسألك عنه في .. انه في mentionفي ..
+
+857
+01:08:05,450 --> 01:08:12,230
+يعني مقال قصير أو ممكن في ال .. في ال in the blank
+
+858
+01:08:12,230 --> 01:08:17,550
+في المصطلح أو حتى في ال mcq في ال .. الاختيار من
+
+859
+01:08:17,550 --> 01:08:21,970
+المتعدد بتسألي عنهم هدول المعلومات فمهم تحفظيهم كل
+
+860
+01:08:21,970 --> 01:08:29,390
+واحد إيش بيصير فيه مدته واسم للمرحلة طيباحنا حكينا
+
+861
+01:08:29,390 --> 01:08:34,090
+إنه لتيوب بيكون مدرج بحيث إنه أعلى تدريج بيكون
+
+862
+01:08:34,090 --> 01:08:38,490
+لتحت و ال zero فوق، تمام؟ و أنا بملأ هاي لتيوب
+
+863
+01:08:38,490 --> 01:08:44,380
+فلازم أملأها لعندي ال zero، تمام؟عشان لما أجى أقرأ
+
+864
+01:08:44,380 --> 01:08:49,220
+أقرأ سقوط ال RPCs أقرأها من ال zero لوين لمتى يكون
+
+865
+01:08:49,220 --> 01:08:53,580
+الطرقة سهلة عندي طيب لو نفرض أنه أنا كمية ال blood
+
+866
+01:08:53,580 --> 01:08:56,480
+كانت ماكانتش كافية أن أنا أملأ كل tube بحيث أنه
+
+867
+01:08:56,480 --> 01:09:00,260
+يصل نهاية ال blood عند ال zero mark وصل عند ال
+
+868
+01:09:00,260 --> 01:09:05,340
+واحد mark او ال اتنين ليه قبل ال zero طبعا فمعناها
+
+869
+01:09:05,340 --> 01:09:10,860
+انا عندي volume أقل من ال volume اللي لازم اشتغل
+
+870
+01:09:10,860 --> 01:09:16,290
+عليه بواحد او اتنين مليطبعا لنفرض أنه كانت النتيجة
+
+871
+01:09:16,290 --> 01:09:23,250
+نهائية 18 18 إيش؟ هي يعني 18 كانت ترسيب مع 2 فاقد
+
+872
+01:09:23,250 --> 01:09:28,630
+2 نقص في ال volume طبعا إذا لأ ساعتها عشان أجيب
+
+873
+01:09:28,630 --> 01:09:33,490
+النتيجة الدقيقة بسجل النتيجة 18 نقص 2 ويساوي 16 mm
+
+874
+01:09:33,490 --> 01:09:38,230
+per hourطيب لو كانت ال .. ال .. عين .. يعني مستوى
+
+875
+01:09:38,230 --> 01:09:41,510
+ال blood وصل أقل من .. يعني لتحت الصفر بواحد،
+
+876
+01:09:41,510 --> 01:09:44,950
+باتنين، بتلاتة، باربعة، بشتغل بهذه الطريقة لكن
+
+877
+01:09:44,950 --> 01:09:49,350
+خمسة فأكثر أو أقل من الخمسة حتى يعني أنه تحت
+
+878
+01:09:49,350 --> 01:09:54,030
+الخمسة يعني في فقدان أكبر من خمسة millimeter هذا
+
+879
+01:09:54,030 --> 01:09:59,350
+معناها فبالتالي هيكون النتيجة not valid يعني لا
+
+880
+01:09:59,350 --> 01:10:03,640
+تُأخد بيهاnot .. not considered لا تعتبر يعني ما
+
+881
+01:10:03,640 --> 01:10:09,840
+تأخدش بعين الاعتبار لازم أعيد التجربة طيب فإذا ال
+
+882
+01:10:09,840 --> 01:10:15,010
+..النتيجة إذا كانت يعني إنه إذا كان الحجم تم
+
+883
+01:10:15,010 --> 01:10:18,850
+تعبئته لتحت الخمسة يعني في فاقد أكتر من خمسة
+
+884
+01:10:18,850 --> 01:10:22,490
+فبالتالي لازم أنا أعيد النتيجة if the upper level
+
+885
+01:10:22,490 --> 01:10:26,010
+of blood is below zero due to leakage of blood
+
+886
+01:10:26,010 --> 01:10:29,930
+from the bottom of tube فبالتالي أيضا النتيجة
+
+887
+01:10:29,930 --> 01:10:34,890
+invalid لو كان في عندي نزل مستوى blood عن ال zero
+
+888
+01:10:34,890 --> 01:10:38,750
+نتيجة تسريب احنا حكينا إنه في ال leakage هيكون فيه
+
+889
+01:10:38,750 --> 01:10:44,970
+فقدان لل RBCs أكترمن ال plasma فبالتالي هذا هيأثر
+
+890
+01:10:44,970 --> 01:10:49,350
+على النتيجة ويخليها لا يأخذ بيها، تمام؟ فلازم أعيد
+
+891
+01:10:49,350 --> 01:10:52,970
+الفحص All the sedimentation racks should be
+
+892
+01:10:52,970 --> 01:10:56,170
+equipped with leveling screw and super spirit
+
+893
+01:10:56,170 --> 01:10:59,480
+levelلازم تكون هذه ال .. ال racks اللي أنا
+
+894
+01:10:59,480 --> 01:11:04,700
+بستخدمها في عندها أمان أو ضابط أو إشي يعني يكون
+
+895
+01:11:04,700 --> 01:11:08,700
+دايما أكدلي أنه أنا المستوى اللي بشتغل عليه مستوى
+
+896
+01:11:08,700 --> 01:11:15,440
+أفقي و ال cube عمودي 100% تمام؟ يصنع زاوية 90 درجة
+
+897
+01:11:15,440 --> 01:11:22,240
+mechanical source of error خلل أو مصدر خلل نتيجة
+
+898
+01:11:22,240 --> 01:11:29,090
+mechanical errorطيب إذا زادت تركيز ال editة، طبعا؟
+
+899
+01:11:29,090 --> 01:11:33,730
+إذا زادت تركيز ال editة، طبعا؟ فيعني .. يعني ..
+
+900
+01:11:33,730 --> 01:11:36,690
+إيش يعني زاد تركيز ال editة؟ مش إحنا حكينا
+
+901
+01:11:36,690 --> 01:11:40,270
+القنابيب معلمة، يعني أنا لازم أعبيها لعند علامة
+
+902
+01:11:40,270 --> 01:11:45,360
+معينة، طبعا عبيت القنابيب أقل من العلامةيعني blood
+
+903
+01:11:45,360 --> 01:11:49,720
+نسبته أقل من ما ينبغي بالنسبة لل Edeta يعني ال
+
+904
+01:11:49,720 --> 01:11:53,880
+Edeta زادت، تمام؟ فإذا زادت ال Edeta حكينا إنها
+
+905
+01:11:53,880 --> 01:11:57,620
+بتقلل ال ESR وهي المعلومة مرت علينا قبل هيك طب
+
+906
+01:11:57,620 --> 01:12:01,260
+ليش؟ لإنها بتعمل inhibition ل ال roll-off
+
+907
+01:12:01,260 --> 01:12:06,900
+formation ال ESR بيقل نتيجة inhibition لل roll-off
+
+908
+01:12:06,900 --> 01:12:10,440
+formation لإن ال Edeta إذا زادت هتعمل inhibition
+
+909
+01:12:10,440 --> 01:12:16,000
+لل roll-off formationطيب، أيضًا ال anticoagulant
+
+910
+01:12:16,000 --> 01:12:20,680
+of sodium أجزالات or potassium أجزالات الهيبرين
+
+911
+01:12:20,680 --> 01:12:26,240
+بيعملولي ال RBCs بيسببوا الشرنكش لل RBCs، يعني
+
+912
+01:12:26,240 --> 01:12:31,600
+اضمحلال أو ضمور لل RBCs أو يعني انت هتكرمش ال RBCs
+
+913
+01:12:32,010 --> 01:12:36,390
+فبالتالي حجم ال RBCs ال sediment تبعها هيقل
+
+914
+01:12:36,390 --> 01:12:42,130
+البلازمة هتزيد على حساب ال RBCs يعني إيش؟ أنا بقيس
+
+915
+01:12:42,130 --> 01:12:45,310
+المسافة اللي سقطتها، طب هي المسافة اللي سقطتها؟
+
+916
+01:12:45,310 --> 01:12:48,090
+يعني سقطت مسافة و اضمحلت نتيجة وجود ال
+
+917
+01:12:48,090 --> 01:12:53,610
+anticoagulant، ضمرتطبعا فهذا هيزيد من قيمة EAS R
+
+918
+01:12:53,610 --> 01:12:58,590
+falsely elevated خلل هذا هو مش عالي لإنه في خلل
+
+919
+01:12:58,590 --> 01:13:04,950
+عند المريض لأ هو عالي نتيجة خلل في سحب العين او في
+
+920
+01:13:04,950 --> 01:13:10,590
+اختيار النوع المناسب لل anticoagulantإذا if the
+
+921
+01:13:10,590 --> 01:13:14,590
+ESR stand for more than 60 minutes the result will
+
+922
+01:13:14,590 --> 01:13:17,850
+be falsely elevated وبرضه كمان لو أنا نسيت ل tube
+
+923
+01:13:17,850 --> 01:13:23,350
+أكتر من ساعة ممكن أيضا النتيجة ESR تكون زيادة
+
+924
+01:13:23,350 --> 01:13:28,390
+تمام؟ و هذا طبعا إذا كان أيضا ال .. ال .. الوقت
+
+925
+01:13:28,390 --> 01:13:33,210
+أقل من ساعة هذا برضه كمان ممكن يعملي low value ف
+
+926
+01:13:33,210 --> 01:13:38,670
+.. و invalid يعني ما ..لا يُأخذ بها طبعا mark the
+
+927
+01:13:38,670 --> 01:13:41,890
+increase or decrease in room temperature lead to
+
+928
+01:13:41,890 --> 01:13:45,170
+increase or decrease ESR result احنا حكينا زيادة
+
+929
+01:13:45,170 --> 01:13:49,270
+ال room temperature كل ما زادت درجة الحرارة هتزيد
+
+930
+01:13:49,270 --> 01:13:54,360
+قيمة ال ESRtilting of the ESR tube increasing the
+
+931
+01:13:54,360 --> 01:13:59,020
+ESR إي مالة الأنابيد يعني إنها ما تكون في ال
+
+932
+01:13:59,020 --> 01:14:02,980
+vertical position تكون ميلة هتزود ال ESR وحكينا
+
+933
+01:14:02,980 --> 01:14:07,920
+ليش لإنه بيصير عندي مساحة سطح بسبب الميلان هذا
+
+934
+01:14:07,920 --> 01:14:11,920
+بيصير عندي مساحة سطح لل roll off formation أكبر
+
+935
+01:14:11,920 --> 01:14:17,820
+طبعا؟ طيبBubble in blood cause invalid result
+
+936
+01:14:17,820 --> 01:14:22,840
+فقعات من الهواء في الأنابيب
+
+937
+01:14:23,760 --> 01:14:27,340
+يعني برضه كمان هتأثر على النتيجة هتعطيني invalid
+
+938
+01:14:27,340 --> 01:14:32,120
+result و .. لإنه ايضا انا يعني بقيس مسافة فلو
+
+939
+01:14:32,120 --> 01:14:35,680
+المسافة هذه مشغولة يعني في فنوسها bubble يعني فإذا
+
+940
+01:14:35,680 --> 01:14:40,320
+القراعة تبعتي مش هتكون دقيقة fibrin clot present
+
+941
+01:14:40,320 --> 01:14:43,500
+in the blood invalidate result برضه كمان أنه يصير
+
+942
+01:14:43,500 --> 01:14:47,390
+في عندى clotting في عينة الدمأيضا هيأثر على
+
+943
+01:14:47,390 --> 01:14:51,230
+النتيجة، هيخلي نتيجة لا أخذ بها، وهذا ال fiber
+
+944
+01:14:51,230 --> 01:14:54,290
+nickelot ممكن تظهر نتيجة ان انا ماعمل mixing
+
+945
+01:14:54,290 --> 01:14:58,260
+للعينة مع ال anticoagulant بشكل كافيفصار في
+
+946
+01:14:58,260 --> 01:15:02,640
+clotting يعني aggregation لل RBCs بدون صار في
+
+947
+01:15:02,640 --> 01:15:07,020
+aggregation يعني هترسب بسرعة و هده يعني نتيجة خلال
+
+948
+01:15:07,020 --> 01:15:12,200
+error the ESR should be set within two hours of
+
+949
+01:15:12,200 --> 01:15:16,520
+blood collection أنا لازم أشتغل ال ESR خلال ساعتين
+
+950
+01:15:16,520 --> 01:15:21,180
+من جمع العينة إذا أنا بشتغل يعني إذا اعتمدت ال
+
+951
+01:15:21,180 --> 01:15:26,120
+EDTA anticoagulant هممكن يعطيني ست ساعاتيعني وقت
+
+952
+01:15:26,120 --> 01:15:32,380
+يعني اللي بنحكي عنه يعني الوقت الفاصل ما بين جمع
+
+953
+01:15:32,380 --> 01:15:37,440
+العينة ويعني أنه أنا أجي أشتغلها ممكن معايا لحد ست
+
+954
+01:15:37,440 --> 01:15:40,300
+ساعات إذا كانت العينة refrigerated باستخدام ال
+
+955
+01:15:40,300 --> 01:15:45,480
+editor تمام؟ باستخدام set rate؟ لأ ساعتين تمام،
+
+956
+01:15:45,480 --> 01:15:47,660
+هلأ ال windrop ESR
+
+957
+01:15:50,850 --> 01:15:55,510
+طبعا انبوبة windrup more sensitive لو ال ESR له
+
+958
+01:15:55,510 --> 01:16:03,970
+طبعا يعني لو ال ESR قليل اي .. يعني اي تغير في
+
+959
+01:16:03,970 --> 01:16:09,190
+قيمته هي .. هي .. يعني هي ال tube هتتمكن من انها
+
+960
+01:16:09,190 --> 01:16:14,010
+تكشف ليه عنه طبعا اذا sensitive اي تغير في ال ESR
+
+961
+01:16:14,010 --> 01:16:18,070
+اللي قيمته قليلة ال windrup method يعني افضل له
+
+962
+01:16:19,650 --> 01:16:25,710
+ممكن تعمله detection أما الوستيجرين method تمام هي
+
+963
+01:16:25,710 --> 01:16:30,630
+more accurate لو كان ال ESR عالي تمام؟ و
+
+964
+01:16:30,630 --> 01:16:33,510
+الوستيجرين method has been chosen as the standard
+
+965
+01:16:33,510 --> 01:16:36,110
+يعني هي ال standard method و المتمدة أكتر
+
+966
+01:16:36,110 --> 01:16:40,330
+الوستيجرين methodWhen reading the ESR result on
+
+967
+01:16:40,330 --> 01:16:43,930
+blood with an extremely high YBCs or platelet
+
+968
+01:16:43,930 --> 01:16:48,050
+count, the buffy coat should be excluded نفس فكرة
+
+969
+01:16:48,050 --> 01:16:51,450
+الهيموتقرات لما أنا كنت بقيس الهيموتقرات كنت بقيس
+
+970
+01:16:51,450 --> 01:16:56,510
+نسبة ال RBCs فقط بدون ال buffy coatطبعا و ايضا لما
+
+971
+01:16:56,510 --> 01:17:00,290
+بدي اقيس ال ESR الترسيب هشوف مسافة اللي قطعتها
+
+972
+01:17:00,290 --> 01:17:03,550
+يعني مسافة البلازمة او الحجم .. المسافة اللي
+
+973
+01:17:03,550 --> 01:17:11,010
+احتلتها البلازمة طبعا و .. يعني هذا الاشي بدون ما
+
+974
+01:17:11,010 --> 01:17:13,790
+انا اقرأ ال buffy quote يعني should be excluded
+
+975
+01:17:13,790 --> 01:17:17,290
+يعني ال buffy quote يعني بيكون خارج القراءة تبعتي
+
+976
+01:17:17,290 --> 01:17:21,450
+if there is poor separation of the RBCs and the
+
+977
+01:17:21,450 --> 01:17:27,660
+plasma layerthis may be due to increased number of
+
+978
+01:17:27,660 --> 01:17:31,300
+reticulocyte و بسمي stratified sedimentation هلأ
+
+979
+01:17:31,300 --> 01:17:37,200
+إيش معنى هذه الجملة؟ هلأ في نوع من الخلائق أو يعني
+
+980
+01:17:37,200 --> 01:17:42,720
+مرحلة من مراحل نضوج ال RBCs بسميها reticulocyte
+
+981
+01:17:42,720 --> 01:17:46,900
+مرحلة ال reticulocyte هي مرحلة ما قبل خروجها
+
+982
+01:17:46,900 --> 01:17:51,990
+مباشرة على ال circulationطبعا هي نفس الشكل يعني
+
+983
+01:17:51,990 --> 01:17:55,490
+الخلية ال RBCs احنا نعرف انها أثناء نضوجها بتمر
+
+984
+01:17:55,490 --> 01:17:59,850
+بمراحل في ال bone marrow أخر مرحلة في ال bone
+
+985
+01:17:59,850 --> 01:18:02,550
+marrow بتكون ال reticulocyte و بعدين بتطلع على ال
+
+986
+01:18:02,550 --> 01:18:07,050
+circulation طبعا ال reticulocyte بتشبه الخلية اللي
+
+987
+01:18:07,050 --> 01:18:09,810
+موجودة في ال circulation الخلية اللي احنا بنعرفها
+
+988
+01:18:09,810 --> 01:18:17,040
+اللي هي شكلهاتمام؟ ولكن بيكون فيها يعني زي شبكة
+
+989
+01:18:17,040 --> 01:18:21,620
+RNA لسه ما اتخلصت من ال RNA تبعها بتطلع .. يعني
+
+990
+01:18:21,620 --> 01:18:24,700
+نسبة ال reticulocyte بشكل طبيعي بالجسم الإنسان
+
+991
+01:18:24,700 --> 01:18:29,280
+واحد في المية واحد لا اتنين في المية .. هك يعني و
+
+992
+01:18:29,280 --> 01:18:33,980
+بتطلع هاي ال reticulocyte على الدم بتاخد لها يوم و
+
+993
+01:18:33,980 --> 01:18:38,440
+بتندج يعني بت .. خلاص بتختفي ال RNA و بتصير ال
+
+994
+01:18:38,440 --> 01:18:44,330
+RBCS طبيعيةطيب increase number of reticulocyte متى
+
+995
+01:18:44,330 --> 01:18:49,710
+بيزيد هذا العدد من ال reticulocyte؟ متى بتزيد نسبة
+
+996
+01:18:49,710 --> 01:18:53,610
+ال reticulocyte في ال circulation و بيزيد نسبتها و
+
+997
+01:18:53,610 --> 01:18:57,930
+بيزيد إنتاجها على ال .. يعني او خروجها من البون
+
+998
+01:18:57,930 --> 01:19:05,250
+مرة؟نتيجة ال blood loss يعني فقدان دم أنيميا تكسر
+
+999
+01:19:05,250 --> 01:19:10,750
+خلايا ال RBCs أي أمرض ممكن ي .. ي .. ينشأ عنه
+
+1000
+01:19:10,750 --> 01:19:15,170
+فقدان RBCs ممكن ال bone marrow يروح يصير ضغط عليه
+
+1001
+01:19:15,170 --> 01:19:19,090
+أنه والله بدنا تصنيع أكتر يعني طلع أكتر RBCs احنا
+
+1002
+01:19:19,090 --> 01:19:22,990
+عم نفقد RBCs تمام؟ فبالتالي ممكن يطلع ال
+
+1003
+01:19:22,990 --> 01:19:25,410
+reticulosa فيها مش ناضجة بس يلا ده بروح حالكوا
+
+1004
+01:19:25,410 --> 01:19:29,920
+فيها شوية يعني هنا بدها يوموا تندجطبعا؟ طيب هذه ال
+
+1005
+01:19:29,920 --> 01:19:34,420
+reticulocyte مع خلايا الدم الطبيعية اللي موجودة في
+
+1006
+01:19:34,420 --> 01:19:39,100
+الدم يعني مختلفة في اختلاف ما بينهم في الكثافة في
+
+1007
+01:19:39,100 --> 01:19:44,840
+الحجم في ال .. في الوزم يعني هي نفس الشكل تقريبا
+
+1008
+01:19:44,840 --> 01:19:48,980
+بس يعني حجمها أكبر شوية كثافتها مختلفة فقل وزمها
+
+1009
+01:19:48,980 --> 01:19:54,260
+مختلفطبعا فلما تيجي ترسب مابترسبش كلها بطبقة واحدة
+
+1010
+01:19:54,260 --> 01:19:58,900
+الخلايا بيصير في عندي ترسيب مخطط stratified
+
+1011
+01:19:58,900 --> 01:20:03,280
+sedimentation يعني طبقة خلايا بلازمة طبقة خلايا
+
+1012
+01:20:03,280 --> 01:20:10,100
+بلازمة و هكذا طبعا فبيصير في عندي يعني زي البوب
+
+1013
+01:20:10,100 --> 01:20:15,500
+بيصير مخططة RBCs أحمر أصفر أحمر أصفر زي هيك طبعا
+
+1014
+01:20:15,500 --> 01:20:19,650
+RBCs بلازمة RBCs بلازمة و هكذانتيجة وجود
+
+1015
+01:20:19,650 --> 01:20:24,710
+reticulocytosis او زيادة في نسبة ال reticulocyte
+
+1016
+01:20:24,710 --> 01:20:28,370
+the internal bore and the length of a graduated
+
+1017
+01:20:28,370 --> 01:20:30,670
+scale on the western pipe are critical
+
+1018
+01:20:30,670 --> 01:20:34,990
+measurements حكينا قطرها و طولها للأنبوب لازم يكون
+
+1019
+01:20:34,990 --> 01:20:39,510
+standard انا بستخدم قنابيب خاصة لان القنابيب اذا
+
+1020
+01:20:39,510 --> 01:20:43,940
+كانت مثلا اذا زادرفعها يعني مافيش مساحة سطح كافية
+
+1021
+01:20:43,940 --> 01:20:48,700
+لل .. لل .. لل ESR ممكن يقل لإن مافيش مساحة سطح
+
+1022
+01:20:48,700 --> 01:20:51,860
+كافية لل roll of formation بيقل ال ESR و نبوب
+
+1023
+01:20:51,860 --> 01:20:55,640
+سميكة يعني مساحة سطح أكبر لل roll of formation
+
+1024
+01:20:55,640 --> 01:21:00,680
+هيزيد ال ESR تمام؟ فبالتالي أنه لازم تكون الأنابيب
+
+1025
+01:21:00,680 --> 01:21:03,580
+standard لما أجي أشتغل فيها عشان أطلع نتائج
+
+1026
+01:21:03,580 --> 01:21:04,640
+accurate and precise
+
+1027
+01:21:09,520 --> 01:21:16,080
+disposable ESR مرت معانا هذه الجملة أو هذا المصطلح
+
+1028
+01:21:16,080 --> 01:21:21,580
+disposable طبعا و معنا استخدام لمرة واحدة فقط و
+
+1029
+01:21:21,580 --> 01:21:25,160
+بعدين هيطلف disposable يعني one use لما أحكيلكم
+
+1030
+01:21:25,160 --> 01:21:29,560
+هذه الأداة disposable إيش يعني؟ يعني استخدميها و
+
+1031
+01:21:29,560 --> 01:21:34,020
+بعدين قتلي فيها ارميها طبعا تخلصي منها هي ما بنرجع
+
+1032
+01:21:34,020 --> 01:21:37,990
+نستخدمها مرة تانيةهلأ و في ون تروب و في وستي جريني
+
+1033
+01:21:37,990 --> 01:21:42,230
+تيوب زجاجية و بنغسلها و بنعقمها بعد كل استخدام و
+
+1034
+01:21:42,230 --> 01:21:45,690
+بنرجع نستخدمها مرة تانية لكن في أنابيب بلاستيكية
+
+1035
+01:21:45,690 --> 01:21:49,330
+زي اللي موجودة في الخلفية هاي فاستخدام لمرة واحدة
+
+1036
+01:21:49,330 --> 01:21:53,490
+فقط و يتم التخلص منها هذه بسميها disposable ESR
+
+1037
+01:21:53,490 --> 01:21:59,610
+فعندي commercial kit كتات تجارية صارت تجيني متاحة
+
+1038
+01:21:59,610 --> 01:22:04,550
+صارت now available لال disposable ESRطبعا ال civil
+
+1039
+01:22:04,550 --> 01:22:07,830
+kit include safety cap for the babies that allow
+
+1040
+01:22:07,830 --> 01:22:10,970
+the blood to precisely fill to the zero بيكون طبعا
+
+1041
+01:22:10,970 --> 01:22:16,490
+في زي صمام موجود في خلايا .. في هذه النبوبة و طبعا
+
+1042
+01:22:16,490 --> 01:22:20,250
+أثناء دخول أنا .. يعني أنا هاي ال tube إلها صمام و
+
+1043
+01:22:20,250 --> 01:22:23,070
+بدخلها داخل عينة الدم تبعتي، انبوبة الدم اللي أنا
+
+1044
+01:22:23,070 --> 01:22:28,040
+شعبتهتمام؟ و هذا الصمام بيسمح انه الارق ..
+
+1045
+01:22:28,040 --> 01:22:32,580
+الانبوبة تفوت و تمتلق بال blood لعند ايش؟ لعند
+
+1046
+01:22:32,580 --> 01:22:36,480
+علامة ال zero هاي شايفين لو انتوا جيتوا هنا نكبر
+
+1047
+01:22:36,480 --> 01:22:42,820
+هاي الصورة و نطلع في الخلفية هاي الانبوب zeroخمسة،
+
+1048
+01:22:42,820 --> 01:22:47,080
+عشرة، تمام؟ إحنا حكينا إن الأنابيب لازم أعبيها لل
+
+1049
+01:22:47,080 --> 01:22:51,940
+zero blood اللي هي زي هيك لعند ال zero أعبيها ما
+
+1050
+01:22:51,940 --> 01:22:56,900
+تكون .. لو كانت وصلت للواحد، للاتنين، للتلقال ت ..
+
+1051
+01:22:56,900 --> 01:23:00,740
+ها دي يعني ها ممكن أنا أحل المشكلة by subtraction
+
+1052
+01:23:00,740 --> 01:23:03,900
+يعني أطرح في النهاية القيمة اللي تطلع معايا من
+
+1053
+01:23:03,900 --> 01:23:10,310
+كمية الفاقد أو النقصفي ال volume طبعا لو كانت يعني
+
+1054
+01:23:10,310 --> 01:23:14,070
+تحت الخمسة يعني في عندي فاقد انا اكتر من خمسة
+
+1055
+01:23:14,070 --> 01:23:19,770
+تمام؟ فبالتالي هذه invalid result طيب احنا بنحكي
+
+1056
+01:23:19,770 --> 01:23:24,440
+انه في ال disposable اي أسئار tube فيها صمامبيسمح
+
+1057
+01:23:24,440 --> 01:23:29,160
+مرور ل tube داخل عينة الدم ويرتفع فيها الدم الى
+
+1058
+01:23:29,160 --> 01:23:33,520
+نسبة ال .. ال zero level تمام بشكل دقيق و هذا
+
+1059
+01:23:33,520 --> 01:23:37,360
+بيخلي ان ال by بتتصير a closed system تصير نظام
+
+1060
+01:23:37,360 --> 01:23:42,080
+مغلق .. مغلق يعني هذا طبعا ال .. ال cab او ال ..
+
+1061
+01:23:44,050 --> 01:23:49,570
+الصمام بي .. بيضل ثابت على ال tube على عينة الدم و
+
+1062
+01:23:49,570 --> 01:23:53,090
+بعدين هك أنا بحطهم في الركات بهذا الشكل و الرك
+
+1063
+01:23:53,090 --> 01:23:57,470
+بيكون مدرج الخلفية بحسب ساعة على ال stop watch و
+
+1064
+01:23:57,470 --> 01:24:02,730
+بعدين هك باجي بقرأ النتيجة تبعتي، تمام؟ فهي .. هذا
+
+1065
+01:24:02,730 --> 01:24:08,190
+معناه ال ESR أو فكرة ال ESR ان شاء الله بس نرجع
+
+1066
+01:24:08,190 --> 01:24:14,930
+للمعامل بنطبقها عملي و حكينا انههتشتغله بطريقة
+
+1067
+01:24:14,930 --> 01:24:19,250
+مختلفة يعني هعطيكم قنابيب زجاجية بتعبوها blood at
+
+1068
+01:24:19,250 --> 01:24:23,730
+level معين و بعد هيك نجلسها على الراك و نحسب طبعا
+
+1069
+01:24:23,730 --> 01:24:26,990
+ال kit اللي تحكيلي او تعليمات ال kit او الشركة
+
+1070
+01:24:26,990 --> 01:24:30,290
+المصنعة لل kit اللي تحكيلي احصي بنص ساعة فقط و ليس
+
+1071
+01:24:30,290 --> 01:24:34,890
+ساعة و بعد هيك القنابيب disposable يعني اتخلصي
+
+1072
+01:24:34,890 --> 01:24:39,430
+منهم تمام؟ هذه اللي احنا راح نشتغلها في المختبرات
+
+1073
+01:24:39,430 --> 01:24:47,900
+تجربة نص ساعةو ان شاء الله إذا الله رد إلنا عودة و
+
+1074
+01:24:47,900 --> 01:24:53,240
+بنشتغل العملية هيك بنكون وصلنا لنهاية محاضرتنا
+
+1075
+01:24:53,240 --> 01:24:58,300
+لليوم و لكن في عندي بعض الصور لسه بدي أعرضها معاكم
+
+1076
+01:24:58,300 --> 01:25:01,360
+هي هنا في عندي هاي الصورة
+
+1077
+01:25:04,130 --> 01:25:09,610
+شايفين؟ هي هنا عندي fibrinogen البروتين تمام و زي
+
+1078
+01:25:09,610 --> 01:25:13,230
+مثلا عندي antibodies globulin بروتين هذه
+
+1079
+01:25:13,230 --> 01:25:19,230
+البروتينات بتروح إيش بتعمل؟ بتحيط ال RBC بتقلل من
+
+1080
+01:25:19,230 --> 01:25:23,790
+الشحنة السالبة، بتقلل من ال zeta potential تمام؟
+
+1081
+01:25:23,790 --> 01:25:28,360
+هي added positive chargeبتضيف شحنة موجة بقى
+
+1082
+01:25:28,360 --> 01:25:32,620
+neutralize negative charge بتعادل الشحنة السالي
+
+1083
+01:25:32,620 --> 01:25:37,600
+بقى ال zeta potential بيقل reduce zeta potential
+
+1084
+01:25:37,600 --> 01:25:41,500
+يعني هتقلل ال repulsion forces و تقلل المسافة ما
+
+1085
+01:25:41,500 --> 01:25:46,310
+بين ال RBCs مما يؤدي إلى ايش؟ تجمع ال RBCsstack of
+
+1086
+01:25:46,310 --> 01:25:50,810
+RBCs يعني rollout formation وهذا هيقدّي إلى إيش؟
+
+1087
+01:25:50,810 --> 01:25:55,410
+سرعة سقوط ال RBCs إيش يعني rollout؟ يعني إنها تكون
+
+1088
+01:25:55,410 --> 01:26:00,150
+ملتصقة مع بعض هالبعض like stack coins تمام؟
+
+1089
+01:26:00,150 --> 01:26:04,650
+ومذكورة في أحد ال slides
+
+1090
+01:26:22,920 --> 01:26:26,620
+مدكور في أحد ال slides في ال .. ال comments تحت
+
+1091
+01:26:26,620 --> 01:26:30,340
+إيش يعني rollout formation؟ rollout formation يعني
+
+1092
+01:26:30,340 --> 01:26:34,480
+RBCs aggregate like stack coins هورجيكم إياها و
+
+1093
+01:26:34,480 --> 01:26:36,100
+أختم المحاضرة ان شاء الله
+
+1094
+01:27:45,840 --> 01:27:56,900
+rolox معناها RBCs aggregate like
+
+1095
+01:27:56,900 --> 01:28:00,620
+stack
+
+1096
+01:28:00,620 --> 01:28:03,020
+coins
+
+1097
+01:28:04,420 --> 01:28:08,640
+زي القطع المعدنية أو اللي هي أو العملة الملتصقة مع
+
+1098
+01:28:08,640 --> 01:28:12,520
+بعضها البعض اللي هي يعني قائص أفطة الشواكر اللي
+
+1099
+01:28:12,520 --> 01:28:16,880
+أنا دربتلكم مثال عليها تمام؟ و هيك أيضا بت .. و
+
+1100
+01:28:16,880 --> 01:28:20,460
+هيك تعريفها في الكتب يعني RBCs aggregate like
+
+1101
+01:28:20,460 --> 01:28:24,000
+stack of coins يعني زي قطع معدنية منزلقة على بعضها
+
+1102
+01:28:24,000 --> 01:28:25,780
+البعض أو ملتصقة ببعضها البعض
+
+1103
+01:28:28,590 --> 01:28:32,730
+وصلنا لنهاية محاضرتنا لليوم، يعطيكم ألف عافية
+
+1104
+01:28:32,730 --> 01:28:34,010
+وموفقين بإبن الله
+
diff --git a/PL9fwy3NUQKwY9jl8xw-bWkVZkWcGGyspd/C6AH0Ptcsrk.srt b/PL9fwy3NUQKwY9jl8xw-bWkVZkWcGGyspd/C6AH0Ptcsrk.srt
new file mode 100644
index 0000000000000000000000000000000000000000..47c85326d7581076c3467285828a5b52ff57e957
--- /dev/null
+++ b/PL9fwy3NUQKwY9jl8xw-bWkVZkWcGGyspd/C6AH0Ptcsrk.srt
@@ -0,0 +1,2428 @@
+1
+00:00:05,480 --> 00:00:09,460
+السلام عليكم ورحمة الله وبركاته. نستكمل مع بعض إن
+
+2
+00:00:09,460 --> 00:00:13,080
+شاء الله الجزء الثاني من المحاضرة بعنوان الـ venipuncture
+
+3
+00:00:13,080 --> 00:00:17,080
+(venipuncture) أي اللي هو الحصول على عينة من الوريد الـ
+
+4
+00:00:17,080 --> 00:00:20,560
+Venipuncture must be performed with care. يعني لازم
+
+5
+00:00:20,560 --> 00:00:27,020
+أنا لما بدي أجري أو بدي أحصل على عينة دم، لازم أحصل
+
+6
+00:00:27,020 --> 00:00:30,560
+عليها بعناية. إذا .. إذا بدي أحصل على عينة دم من
+
+7
+00:00:30,560 --> 00:00:35,440
+الوريد، أحصل عليها بعناية. ليش؟ لأنه the vein of the
+
+8
+00:00:35,440 --> 00:00:38,640
+patient are the main source of blood for testing
+
+9
+00:00:38,640 --> 00:00:42,740
+and the entry point for medication solutions and
+
+10
+00:00:42,740 --> 00:00:47,160
+blood transfusions. يعني لأن الوريد، الوريد
+
+11
+00:00:47,160 --> 00:00:51,440
+للمريض هو الـ .. الـ .. المكان اللي بيسير من خلاله
+
+12
+00:00:51,440 --> 00:00:56,380
+contact مع المريض، منه بيتم عملية الـ testing
+
+13
+00:00:56,380 --> 00:01:01,240
+يعني إحنا بنحصل على عينة و .. يعني بيصير إجراء
+
+14
+00:01:01,240 --> 00:01:04,980
+فحوصات عليها أيضا. هو الـ entry point للـ medication
+
+15
+00:01:04,980 --> 00:01:11,940
+من خلاله بيتم إدخال أدوية أو محاليل للمريض، وأيضا
+
+16
+00:01:11,940 --> 00:01:17,020
+من خلال الـ .. الوريد أيضا يتم نقل دم إذا استدعى
+
+17
+00:01:17,020 --> 00:01:22,360
+الأمر. فبالتالي، إذا لازم أنا أتعامل مع الوريد بحذر
+
+18
+00:01:22,360 --> 00:01:26,020
+علشان أنه أنا ما أقعد في المريض، because there are
+
+19
+00:01:26,020 --> 00:01:29,760
+only a limited number of easily accessible veins. لأنه
+
+20
+00:01:29,760 --> 00:01:34,200
+أنا في عندي عدد محدود من الأوردة اللي سهل الوصول
+
+21
+00:01:34,200 --> 00:01:37,400
+إليها في المريض. طبعا، it is important that
+
+22
+00:01:37,400 --> 00:01:40,860
+everything be done to preserve their good
+
+23
+00:01:40,860 --> 00:01:44,080
+condition and availability. طبعا عشان أنا في عندي
+
+24
+00:01:44,080 --> 00:01:47,860
+عدد محدود من الأوردة، لازم أضمن أنه كل شيء عم
+
+25
+00:01:47,860 --> 00:01:53,500
+بيشتغل أنا بحضره أو بشتغله، في .. أنه يكون هو يعني
+
+26
+00:01:53,500 --> 00:01:58,760
+بأحسن ظروفه، وبـ .. ضامن الـ quality تبعته، والـ .. والـ
+
+27
+00:01:58,760 --> 00:02:02,160
+الـ availability تبعته. يعني الـ good practice طبعا
+
+28
+00:02:02,160 --> 00:02:05,520
+يعني الـ good practice يعني أن أنا لما أسحب عينة
+
+29
+00:02:05,520 --> 00:02:10,620
+الدم، أسحبها بـ .. بـ .. اتباع التعليمات بحذافيرها
+
+30
+00:02:10,620 --> 00:02:14,580
+بحيث أني ما اضطر أن أنا أبحث عن وريد آخر. العدد
+
+31
+00:02:14,580 --> 00:02:19,280
+محدود. تمام. Part of this responsibility lies on the
+
+32
+00:02:19,280 --> 00:02:22,700
+medical technologist. طبعا الجزء من هذه العملية
+
+33
+00:02:22,700 --> 00:02:27,840
+بيقع على كاهل أخصائيي العلوم الطبية المخبرية، أو حتى
+
+34
+00:02:27,840 --> 00:02:31,900
+كمان الجزء الكبير، إذا ما كانت المسؤولية كلها. The
+
+35
+00:02:31,900 --> 00:02:35,540
+ideal procedure is to have the patient lie down.
+
+36
+00:02:35,540 --> 00:02:40,020
+أفضل طريقة في الحصول على عينة دم من الوريد، أن أنا
+
+37
+00:02:40,020 --> 00:02:45,960
+أخلي المريض يستلقي. If this is not possible، طبعا
+
+38
+00:02:45,960 --> 00:02:54,720
+إذا مش متاح سرير، أو يعني كرسي يسمح له أنه يتمدد
+
+39
+00:02:54,720 --> 00:02:57,920
+the patient should sit in a sturdy, comfortable
+
+40
+00:02:57,920 --> 00:03:02,280
+chair with his or her arm firmly supported on the
+
+41
+00:03:02,280 --> 00:03:07,180
+table or chair arm, and is easily accessible to the
+
+42
+00:03:07,180 --> 00:03:11,000
+technologist. هلأ إذا ما كان متاح أن يكون في عندي
+
+43
+00:03:11,000 --> 00:03:15,820
+سرير، أو يكون في عندي كرسي يستلقي عليه المريض.
+
+44
+00:03:15,820 --> 00:03:19,060
+طبعا في أحياناً كراسي بيكون درجها ممكن أنه
+
+45
+00:03:19,060 --> 00:03:22,680
+يتمدد عليه، يعني أنه ينفرج درج الكرسي فإنه يتمدد
+
+46
+00:03:22,680 --> 00:03:27,140
+عليه. ساعتها، إذا ما كان هذا الشيء متاح، أنا بدي
+
+47
+00:03:27,140 --> 00:03:31,520
+أجلس المريض على كرسي مريح وثابت، وطبعاً مثل ما
+
+48
+00:03:31,520 --> 00:03:36,110
+حكينا الـ .. في الجزء الأول، أنه ما يكونش الكرسي عالي
+
+49
+00:03:36,110 --> 00:03:41,250
+ويكون طبعاً لازم يكون إله درج، و .. أساس إنه يمسك
+
+50
+00:03:41,250 --> 00:03:44,410
+المريض منيح، في حال لو هو عليه أو فقد وعيه، إن ما
+
+51
+00:03:44,410 --> 00:03:48,910
+ييجي على الأرض وتكون وجهته محددة. حكينا، تمام؟ طيب.
+
+52
+00:03:48,910 --> 00:03:53,290
+A patient should never stand or .. or sit on a
+
+53
+00:03:53,290 --> 00:03:57,150
+high stool during any process of blood collection.
+
+54
+00:03:57,150 --> 00:04:02,670
+مش لازم الـ .. الـ .. المريض يجلس على كرسي عالي
+
+55
+00:04:02,670 --> 00:04:07,090
+أثناء عملية سحب الدم، عشان إحنا حكينا كل ما زودت
+
+56
+00:04:07,090 --> 00:04:11,130
+المسافة بينه وبين الأرض، هتكون الـ .. يعني الـ إصابة
+
+57
+00:04:11,130 --> 00:04:16,540
+أخطر. تمام؟ طيب، وطبعاً لازم الـ .. المريض بس يجلس على
+
+58
+00:04:16,540 --> 00:04:23,180
+الكرسي اللي هو مريح وآمن، وبتوفر فيه كل الشروط.
+
+59
+00:04:23,180 --> 00:04:28,880
+لازم إنه يـ .. يفرد إيده على الـ .. الـ bench أو الـ
+
+60
+00:04:28,880 --> 00:04:34,180
+طاولة، أو إيدي الكرسي، بحيث إنها تكون إنه سهل
+
+61
+00:04:34,180 --> 00:04:37,640
+الـ technologist يصل لإيده، ويبحث عن الوريد المناسب
+
+62
+00:04:37,640 --> 00:04:42,070
+لحتى يسحب منه العينة. إيش هي الـ reagents والـ
+
+63
+00:04:42,070 --> 00:04:48,290
+equipment اللي لازم أنا أستخدمها؟ Tourniquet، تمام؟
+
+64
+00:04:48,290 --> 00:04:53,770
+وطبعاً tourniquet إيش يعني؟ يعني رباطة علشان تعملي
+
+65
+00:04:53,770 --> 00:04:58,730
+ضغط على الـ .. منطقة الوريد، بحيث يبرز الوريد ويسهل
+
+66
+00:04:58,730 --> 00:05:03,620
+إني أنا أحصل على عينة من الوريد. Alcohol wipes.
+
+67
+00:05:03,620 --> 00:05:07,520
+طبعاً لازم يكون في عندي قطن مغموس بالكحول 70%.
+
+68
+00:05:07,520 --> 00:05:11,800
+أيزوبربانول أو 70% إيثانول. Gauze sponge. طبعاً لازم
+
+69
+00:05:11,800 --> 00:05:18,940
+يكون في عندي قطن مثلاً، أو شاش. Adhesive bandage. حكاية
+
+70
+00:05:18,940 --> 00:05:26,020
+لازم يكون في عندي لاصق، تمام. بلاستر، ولازم يكون في
+
+71
+00:05:26,020 --> 00:05:30,560
+عندي gloves. إذا Nitrile أو Latex أو Vinyl حسب إيش
+
+72
+00:05:30,560 --> 00:05:35,140
+النوع المتاح أو متوفر. طبعاً، sterile، disposable
+
+73
+00:05:35,140 --> 00:05:39,760
+syringe. هاي كلمة disposable اللي معناها أنه
+
+74
+00:05:43,030 --> 00:05:47,390
+هي كلمة disposable اللي معناها أنه الشيء يستخدم
+
+75
+00:05:47,390 --> 00:05:51,630
+لمرة واحدة فقط، تمام؟ طيب، لازم يكون في عندي
+
+76
+00:05:51,630 --> 00:05:55,570
+سرنجات بستخدمها لمرة واحدة فقط، disposable syringe
+
+77
+00:05:55,570 --> 00:06:01,170
+وأيضا disposable needle، والإبرة، تمام؟ طيب، the
+
+78
+00:06:01,170 --> 00:06:04,330
+choice of needle depends on the size of the vein.
+
+79
+00:06:04,330 --> 00:06:10,660
+طبعاً الـ needle هي بتكون بأحجام. إحنا لما بنختار
+
+80
+00:06:10,660 --> 00:06:15,980
+الإبرة، بتكون بأحجام. أنا بختارها حسب حجم الوريد،
+
+81
+00:06:15,980 --> 00:06:19,700
+إذا الوريد رفيع، بختار حجم الـ .. الـ needle أنه
+
+82
+00:06:19,700 --> 00:06:24,080
+يكون رفيع، تمام؟ تكون الـ needle رفيعة. The most
+
+83
+00:06:24,080 --> 00:06:29,720
+commonly used needles. طبعاً الـ needle بيعبر عن قطرها
+
+84
+00:06:29,720 --> 00:06:33,940
+أو عن حجمها، السمك تبعها، رقتها، أو هل هي رفيعة أو
+
+85
+00:06:33,940 --> 00:06:39,790
+سميكة، بيعبر عنه برقم اسمه gauge number، تمام؟ Gauge
+
+86
+00:06:39,790 --> 00:06:47,170
+number، وأيضاً كمان طبعاً بيعبر أيضاً عن .. عن
+
+87
+00:06:47,170 --> 00:06:52,710
+طولها. يعني كمان الـ gauge number.  فهلأ الـ .. زي ما
+
+88
+00:06:52,710 --> 00:06:56,490
+إنتوا شايفين، الـ gauge number عشرين، واحد وعشرين،
+
+89
+00:06:56,490 --> 00:07:00,450
+واثنين وعشرين، تمام؟ هدول الـ most commonly used
+
+90
+00:07:01,540 --> 00:07:05,860
+طبعاً هنا بحكيلك أنه the higher the gauge number
+
+91
+00:07:05,860 --> 00:07:11,480
+the smaller the inner diameter. يعني كل ما زاد الـ
+
+92
+00:07:11,480 --> 00:07:16,020
+gauge number، كل ما قل الـ diameter، يعني كانت الإبرة
+
+93
+00:07:16,020 --> 00:07:20,170
+أرفع، تمام؟ إحنا لما بنيجي بناخد .. يعني بناخد إبرة
+
+94
+00:07:20,170 --> 00:07:23,630
+بالعضل، بنحب ناخد الـ .. في الإبرة الرفيعة، عشان ما
+
+95
+00:07:23,630 --> 00:07:27,650
+نتوجع، تمام؟ يعني إيش الإبرة الرفيعة؟ يعني الـ
+
+96
+00:07:27,650 --> 00:07:31,750
+gauge number العالي. يعني هدول التلات أرقام، مين
+
+97
+00:07:31,750 --> 00:07:35,550
+هم .. يعني أرفع إبرة فيهم؟ أرفع إبرة هي الـ gauge
+
+98
+00:07:35,550 --> 00:07:39,610
+number إثنين وعشرين، وأسمك إبرة هي الـ gauge عشرين،
+
+99
+00:07:39,610 --> 00:07:43,290
+تمام؟ إذا في علاقة عكسية ما بين السمك، ما بين الـ
+
+100
+00:07:43,290 --> 00:07:48,040
+diameter، القطر تبع الإبرة، وما بين الـ gauge number.
+
+101
+00:07:48,040 --> 00:07:51,500
+تمام؟ The length of the needle is also chosen
+
+102
+00:07:51,500 --> 00:07:55,040
+according to the depth of the vein. برضه كمان أنا
+
+103
+00:07:55,040 --> 00:08:00,440
+بختار طول الإبرة تبعاً لعمق الـ .. إذا كانت الـ .. الـ
+
+104
+00:08:00,440 --> 00:08:05,220
+الـ .. الوريد عميق،  داخل لجوة، تمام؟ بختار إبرة
+
+105
+00:08:05,220 --> 00:08:09,340
+طولها يعني طويلة، عشان أنا أفوت لجوة أكتر، تمام؟ أفوت
+
+106
+00:08:09,340 --> 00:08:14,950
+عمق يعني كبير. إذا كان الوريد سطحي، فبختار إبرة طولها
+
+107
+00:08:14,950 --> 00:08:19,370
+قصير. ولكن الـ most commonly used length هو واحد ونص
+
+108
+00:08:19,370 --> 00:08:22,730
+إنش. يعني إنه يكون طول الإبرة واحد ونص إنش. خلينا
+
+109
+00:08:22,730 --> 00:08:26,830
+بس أنا أعمل skip بس لهذه الـ slide، وأشوف هذه
+
+110
+00:08:26,830 --> 00:08:31,070
+المكونات.السرنج والإبرة. هذه هي السرنجة طبعاً.
+
+111
+00:08:31,070 --> 00:08:35,550
+السرنجة زي ما إنتوا شايفين، مكونة من قطعتين، قطعة
+
+112
+00:08:35,550 --> 00:08:39,950
+متحركة، هذه القطعة المتحركة، وهذه الثابتة. طبعاً
+
+113
+00:08:39,950 --> 00:08:44,930
+القطعة الثابتة بسميها barrel of syringe. طبعاً هذه
+
+114
+00:08:44,930 --> 00:08:49,310
+بتكون عليها تدريج. الـ barrel هذا، هي عند 10 سنتي
+
+115
+00:08:49,310 --> 00:08:53,490
+مثلاً، عشرة مل، فيه خمسة مل، فيه ثلاثة مل، فيه
+
+116
+00:08:53,490 --> 00:08:57,180
+فيه اثنين مل. إذا يعني السرنجات مش كلها نفس
+
+117
+00:08:57,180 --> 00:09:06,200
+الحجم، تمام؟ وهذا الجزء المتحرك بسميه plunger، زي
+
+118
+00:09:06,200 --> 00:09:09,080
+الـ plunger تبع الـ pipet. هو الجزء المتحرك اللي أنا
+
+119
+00:09:09,080 --> 00:09:12,400
+كنت بضغط عليه، بكبس عليه كبسة أولى أو كبسة ثانية
+
+120
+00:09:12,400 --> 00:09:15,540
+علشان آخذ الحجوم، صح؟ فهذا نفس الشيء، الـ plunger
+
+121
+00:09:15,540 --> 00:09:18,980
+هو الجزء المتحرك اللي بيرجع لورا، عشان أنا أسحب
+
+122
+00:09:18,980 --> 00:09:26,220
+الحجم الدم المناسب من الـ .. من الوريد. طبعاً حكينا
+
+123
+00:09:26,220 --> 00:09:30,240
+إنه للسرنجات هذه أحجام، طبعاً أنا بختار حسب حجم
+
+124
+00:09:30,240 --> 00:09:33,100
+العينة اللي بدي إياها. إذا أنا بدي أعمل فحوصات
+
+125
+00:09:33,100 --> 00:09:37,140
+قليلة، بختار حجم عينة قليل، يعني السرنجة قليلة، يعني
+
+126
+00:09:37,140 --> 00:09:40,920
+بنهاية كمان بفضيها في تيوب صغيرة. حكينا عن أحجام
+
+127
+00:09:40,920 --> 00:09:46,610
+للتيوب في المحاضرة الماضية. طيب، إذا هذا ما يتعلق بالـ
+
+128
+00:09:46,610 --> 00:09:51,370
+syringe disposable، باستخدام واحد فقط. يعني برميها
+
+129
+00:09:51,370 --> 00:09:55,330
+بعد ما أنا أسحبت فيها. ما بستخدمها لمريض آخر. وهذه الـ
+
+130
+00:09:55,330 --> 00:09:59,310
+needle أيضاً disposable. الإبرة هاي هي الـ needle زي
+
+131
+00:09:59,310 --> 00:10:03,290
+ما أنتوا شايفين. الإبرة أو الـ needle مكونة من اللي
+
+132
+00:10:03,290 --> 00:10:07,890
+هو الجزء المعدني، وجزء بلاستيكي ملوّن، أزرق
+
+133
+00:10:07,890 --> 00:10:12,250
+أخضر، رمادي، حسب نوع لون الإبرة، تمام؟ طب هذا الـ ..
+
+134
+00:10:12,250 --> 00:10:17,440
+الـ .. الجزء بيكون مكتوب عليه الـ gauge number، طبعاً
+
+135
+00:10:17,440 --> 00:10:22,340
+هي العشرين، هي شايفين أسمك واحدة، تمام؟ وأطول واحدة.
+
+136
+00:10:23,310 --> 00:10:29,170
+عشرين أقل سمكاً، واحد وعشرين أقل سمكاً، أرفع واحدة
+
+137
+00:10:29,170 --> 00:10:33,570
+اللي هي الخمسة وعشرين. إذا كل ما زاد الـ .. الـ ..
+
+138
+00:10:33,570 --> 00:10:37,850
+الـ gauge number، تمام؟ هي عشرين، هي زي ما هذه
+
+139
+00:10:37,850 --> 00:10:42,150
+عشرين نفسها، تمام؟ إذا نفس السمك ونفس الطول، الـ
+
+140
+00:10:42,150 --> 00:10:47,210
+gauge واحد وعشرين أقل سمكاً، الخمسة وعشرين أقل
+
+141
+00:10:47,210 --> 00:10:51,790
+سمكاً، أرفع واحدة، تمام؟ وهي أرفع. فبالتالي، إذا كل ما
+
+142
+00:10:51,790 --> 00:10:55,710
+زاد الـ gauge number، كل ما قل السمك، تمام؟ طيب.
+
+143
+00:10:55,710 --> 00:10:59,930
+الجزء المعدني هذا زي ما إنتوا شايفين، مكون من الـ
+
+144
+00:10:59,930 --> 00:11:03,870
+shaft، اللي هو يعني جزء الإبرة، الإسطوانة تبع
+
+145
+00:11:03,870 --> 00:11:10,990
+الإبرة، وجزء منها مدبّب، وفي فتحة بسميها الـ bevel،
+
+146
+00:11:10,990 --> 00:11:13,970
+الفتحة هذه بسميها الـ bevel of needle، فتحة الـ
+
+147
+00:11:13,970 --> 00:11:17,960
+needle، تمام؟ الـ bevel of needle مهم جداً. هذه
+
+148
+00:11:17,960 --> 00:11:22,760
+الجزيئات.  نعرف تركيبة الـ needle ومكوناتها،
+
+149
+00:11:22,760 --> 00:11:26,700
+جزيئاتها، ونعرف تركيبة السرنج، عشان كمان شوية لما
+
+150
+00:11:26,700 --> 00:11:30,740
+نشرح باقي المحاضرة أو الجزء المتبقي من المحاضرة، أنه
+
+151
+00:11:30,740 --> 00:11:34,220
+حنصير نحكي عن الـ bevel، تمام؟ أذكر الـ bevel كتير
+
+152
+00:11:34,220 --> 00:11:38,310
+لنعرف أن الـ bevel هو فتحة الـ needle، تمام؟ الجزء
+
+153
+00:11:38,310 --> 00:11:42,470
+اللي .. اللي هو الـ .. زي مشطوف، بيكون شفتوا الـ ..
+
+154
+00:11:42,470 --> 00:11:45,950
+شكل المكرونة بالبشاميل، كانت بتكون جاية بزاوية،
+
+155
+00:11:45,950 --> 00:11:50,810
+نفس الشيء، فتحة الـ .. الـ .. الـ needle بتكون جاية
+
+156
+00:11:50,810 --> 00:11:56,870
+بزاوية. تمام. نرجع على الـ slide اللي تركناها. الـ evac-
+
+157
+00:11:56,870 --> 00:12:00,030
+uated، الـ vacutainer system. الـ vacutainer system هو
+
+158
+00:12:00,030 --> 00:12:06,050
+أنه بيكون عندي الـ syringe هذه كبيرة، وطبعاً ويلها
+
+159
+00:12:06,050 --> 00:12:10,590
+needle مشبوك فيها، needle، تمام؟ وهاد الـ .. الـ
+
+160
+00:12:10,590 --> 00:12:14,570
+syringe طبعاً أنا بفوتها في وريد المريض، وبستخدم
+
+161
+00:12:14,570 --> 00:12:19,610
+أنبوب خاصة، بفوتها داخل الـ .. الـ .. داخل الـ syringe
+
+162
+00:12:19,610 --> 00:12:24,300
+هذه طبعاً، وبيصير يعبي فيها مباشرة. إنه .. إنه هذه
+
+163
+00:12:24,300 --> 00:12:31,100
+السرنجة بتكون متصلة بإبرة، الإبرة هذه بتاخد الـ ..
+
+164
+00:12:31,100 --> 00:12:33,900
+يعني جزء منها بيكون في الوريد، وجزء منها بيكون
+
+165
+00:12:33,900 --> 00:12:37,960
+بيخترق الـ tube.  هرجيكم إياها إن شاء الله من خلال
+
+166
+00:12:37,960 --> 00:12:44,140
+video اللي بيشرح عملية السحب. فهتشوفوا بإذن الله
+
+167
+00:12:44,140 --> 00:12:48,700
+كيف إحنا يعني كيف بنستخدم .. بنجمع عينة الدم
+
+168
+00:12:48,700 --> 00:12:51,420
+باستخدام needle وسرنج، أو باستخدام الـ vacutainer
+
+169
+00:12:51,420 --> 00:12:56,040
+system. تمام؟ طيب، الـ vacutainer system هو عبارة عن
+
+170
+00:12:56,040 --> 00:13:01,040
+أنه أنا بستخدم أنابيب evacuated، يعني
+
+223
+00:16:42,210 --> 00:16:45,690
+The cubital or the cephalic vein of the arm are used
+
+224
+00:16:45,690 --> 00:16:50,610
+more frequently. و طبعا إذا كان ال .. ال .. ال rest
+
+225
+00:16:50,610 --> 00:16:54,250
+و ال hand veins are also acceptable. أحيانا بناخد
+
+226
+00:16:54,590 --> 00:17:01,150
+يعني ال .. الأوردة اللي موجودين في قبضة الإيد بس
+
+227
+00:17:01,150 --> 00:17:04,570
+يعني بيفضل أن أنا أبعد عنهم و أتجنبهم لأن هم
+
+228
+00:17:04,570 --> 00:17:08,130
+مؤلمين أحيانا، بكونوا، فالأفضل أن أنا أستخدم في
+
+229
+00:17:08,130 --> 00:17:11,710
+منطقة اللي هي الكوع في ال .. في المنطقة اللي هي
+
+230
+00:17:11,710 --> 00:17:14,970
+اللي بنسميها Anticubital Area، في المنطقة اللي
+
+231
+00:17:14,970 --> 00:17:21,230
+اسمها Anticubital Area, and trace the path of the
+
+232
+00:17:21,230 --> 00:17:25,990
+vein with the index finger. بأصبعك السبابة تحسسي
+
+233
+00:17:25,990 --> 00:17:30,370
+الوريد و حاولي أنك تشوفي مساره و تعرفي اتجاهه. ليش؟
+
+234
+00:17:30,370 --> 00:17:34,370
+علشان أنا من خلال .. أنا بدي أفوت .. أكون محادية
+
+235
+00:17:34,370 --> 00:17:40,230
+لاتجاهه، علشان أفوت صح داخل الوريد، تمام؟ الوريد كيف
+
+236
+00:17:40,230 --> 00:17:44,290
+أنا بحسه؟ بحسه أنه في إشي مطاطي تحت إيدي، تحت إصبع
+
+237
+00:17:44,290 --> 00:17:48,130
+السبابة، يعني أنا لما بضغط عليه بحسه بيطلع و بينزل
+
+238
+00:17:48,130 --> 00:17:54,470
+زي كإني بضغط على أنبوب مطاطي أو على إشي زي مثلا
+
+239
+00:17:54,470 --> 00:18:00,330
+مغيطة، تمام؟ و طبعا إله .. الوريد إله thick wall
+
+240
+00:18:00,330 --> 00:18:08,660
+إذا كان الوريد superficial، إذا كان سطحي، ف .. طبعا
+
+241
+00:18:08,660 --> 00:18:13,720
+يعني لازم أنا أفوت له ب .. إذا بكمية .. يعني أفوت
+
+242
+00:18:13,720 --> 00:18:19,740
+سطحية، إذا كان طبعا الوريد عميق، بدي أفوت بعمق،
+
+243
+00:18:19,740 --> 00:18:23,380
+تمام؟ If superficial veins are not readily
+
+244
+00:18:23,380 --> 00:18:27,080
+apparent، طب لو ما كانش مبين ال superficial veins،
+
+245
+00:18:27,080 --> 00:18:31,120
+إيش بدي أعمل؟ You can force blood into the vein
+
+246
+00:18:31,120 --> 00:18:36,490
+by massaging the arm from wrist to elbow, tap the
+
+247
+00:18:36,490 --> 00:18:41,410
+site with index and second finger, apply warm damp
+
+248
+00:18:41,410 --> 00:18:47,990
+cloth to the site for five minutes. يعني لو أنا ما شوفتش
+
+249
+00:18:47,990 --> 00:18:52,390
+الوريد، معناها إن أنا بدي أضغط على المنطقة أو بدي
+
+250
+00:18:52,390 --> 00:18:56,370
+أعمل massaging يمتد من عند اللي هي ال wrist اللي
+
+251
+00:18:56,370 --> 00:19:05,090
+هي من عند الرسغ لحد الكوع، و بقى، أو ممكن أنا
+
+252
+00:19:05,090 --> 00:19:08,530
+يعني أضغط .. أتَحَسَّس من خلال أني أشوف الوريد
+
+253
+00:19:08,530 --> 00:19:12,510
+بالسبابة، ممكن أنا أُدفِّئ المكان من خلال أني
+
+254
+00:19:12,510 --> 00:19:17,550
+أحط قطعة قماش لمدة خمس دقائق تكون دافئة، أو أني
+
+255
+00:19:17,550 --> 00:19:21,970
+أنزل الإيد لأدنى مستوى الجسم، حكينا أنه ساعتها
+
+256
+00:19:21,970 --> 00:19:26,050
+بيتضَفَّق الدم بفعل الجاذبية لأسفل الإيد و بتبين
+
+257
+00:19:26,050 --> 00:19:30,010
+بتبرز الأوردة، زي ما حكينا أو شرحنا بالمحاضرة أو
+
+258
+00:19:30,010 --> 00:19:33,450
+بالجزء الأول من المحاضرة، هي منطقة الـ Anticubital
+
+259
+00:19:33,450 --> 00:19:36,630
+يا بنات، هي منطقة الكوع، أنا من هنا بسحب عينة
+
+260
+00:19:36,630 --> 00:19:40,410
+الدم، وهنا الرسغ، فأنا ببدأ أعمل massage لو الوريد،
+
+261
+00:19:40,410 --> 00:19:43,670
+بَعمل massage بهذا الشكل، بحيث أنه أنا أحرك الدم
+
+262
+00:19:43,670 --> 00:19:47,510
+بهذا الاتجاه عشان يبرز الوريد عندي هنا، أو أحط قطعة
+
+263
+00:19:47,510 --> 00:19:51,700
+قماش دافئة، طبعا إيش الأوردة المناسبة؟ هاي زي ما
+
+264
+00:19:51,700 --> 00:19:54,700
+إنتوا شايفين، دا قَطُوعها عامل زي .. اللي هي رقم 7
+
+265
+00:19:54,700 --> 00:19:59,220
+اللي هم ال main .. ال median cubital، تمام؟ ال
+
+266
+00:19:59,220 --> 00:20:02,200
+median cubital vein و ال cephalic vein، هدول أفضل
+
+267
+00:20:02,200 --> 00:20:05,500
+أوردة، تمام؟ ممكن أنا أروح على الأوردة اللي هنا
+
+268
+00:20:05,500 --> 00:20:07,960
+اللي على .. اللي ال deep، أو اللي على الأطراف هنا
+
+269
+00:20:07,960 --> 00:20:13,980
+ولكن هذا يعني .. بي .. بيختار .. بيعتمد حسب حالة
+
+270
+00:20:13,980 --> 00:20:17,660
+المريض، حسب إيش الوريد الواضح عنده، أحيانا بتكون
+
+271
+00:20:17,660 --> 00:20:20,320
+اللي على الأطراف هنا مؤلمة، فالأفضل أن أنا أكون في
+
+272
+00:20:20,320 --> 00:20:23,500
+منطقة المنتصف قدر المستطاع، يعني في ال cubital و ال
+
+273
+00:20:23,500 --> 00:20:28,000
+cephalic، تمام؟ هذا هو، مع الممارسة يا بنات، هتِسيرِي
+
+274
+00:20:28,000 --> 00:20:32,880
+تِعرفِي ال anatomy تبع الإيد، تِعرفِي مكان الأوردة منك
+
+275
+00:20:32,880 --> 00:20:36,300
+لحالك، يعني مجرد ما ت .. يعني تفوتي ال needle، بتعرفي
+
+276
+00:20:36,300 --> 00:20:41,530
+على الوريد و بتحصلي على الدم، تمام؟ يعني الإشي بده
+
+277
+00:20:41,530 --> 00:20:45,590
+ممارسة، إن شاء الله هنرجع للمختبرات، هنعلمكم، هو هيسحب
+
+278
+00:20:45,590 --> 00:20:50,470
+قدامكم، هخليكم تسحبوا من بعض، و تجيبوا يعني ناس مثلا
+
+279
+00:20:50,470 --> 00:20:53,790
+متبرعين، volunteers، تسحبوا منهم، هعطيكم أدوات تسحبوا
+
+280
+00:20:53,790 --> 00:20:57,910
+من أهلكم في البيت، فيعني هعلمكم إن شاء الله، أسحب و
+
+281
+00:20:57,910 --> 00:21:03,190
+لما أتأكَّد إنكم يعني أتقنتوا، هخليكم يعني أنه تجرّبوا
+
+282
+00:21:03,190 --> 00:21:07,870
+على إما متطوعين أو حدا من أهلكم، لحتى أنه تتقنوا
+
+283
+00:21:07,870 --> 00:21:11,290
+الإشي أكثر و أكثر، و تمارسوا أكثر و أكثر، تمام؟
+
+284
+00:21:11,290 --> 00:21:15,270
+Performance of vein puncture، أنا هلأ يعني اتعرفت
+
+285
+00:21:15,270 --> 00:21:18,450
+على المنطقة اللي بدي أسحب منها، و عرفت إيش الأدوات
+
+286
+00:21:18,450 --> 00:21:23,380
+اللي بدي أحضّرهم، بدي أُجري ال vein puncture، أول إشي
+
+287
+00:21:23,380 --> 00:21:28,540
+طبعا ال .. ال slide زي ما إنتوا شايفين، مكررة، وردت
+
+288
+00:21:28,540 --> 00:21:31,740
+معانا في ال skin puncture، بدي أتعامل مع المريض
+
+289
+00:21:31,740 --> 00:21:36,260
+بشكل friendly، بشكل calm manner، بهدوء، ما أكون
+
+290
+00:21:36,260 --> 00:21:41,600
+aggressive معه، أحاول إن أنا أكون ودودة، لطيفة معاه،
+
+291
+00:21:41,600 --> 00:21:46,220
+طبعا أريحه، أحاول إن أنا أريحه، إذا كان هو متوتر،
+
+292
+00:21:46,220 --> 00:21:51,370
+أخليه ياخد نفس عميق، يستلقي، يسترخي مثلا،
+
+293
+00:21:51,370 --> 00:21:55,330
+إذا هو خايف من ال .. ال procedure، أحكيله مثلا يلف
+
+294
+00:21:55,330 --> 00:21:57,870
+وجهه على الجهة التانية عشان ما يشوف أنا إيش بعمل،
+
+295
+00:21:57,870 --> 00:22:03,070
+طبعا بدي أخليه يتعاون معايا، بدي أتعرف عليه منيح،
+
+296
+00:22:03,070 --> 00:22:05,850
+identify the patient correctly، و يعرفني إيش يعني،
+
+297
+00:22:05,850 --> 00:22:09,130
+كيف أنا أتعرف على المريض منيح و أتأكَّد إن هو هذا
+
+298
+00:22:09,130 --> 00:22:12,150
+المريض الصح، مع ال request الصح، عشان أسحب العينة
+
+299
+00:22:12,150 --> 00:22:17,430
+الصح، و بدي أتعامل مع الجزء اللي خاص فيا بال request
+
+300
+00:22:17,430 --> 00:22:20,950
+form، اللي هو يعني ال date و ال time و ال initial
+
+301
+00:22:20,950 --> 00:22:26,210
+تبعي، أيضا بدي أتأكَّد هل المريض مستوفي لشروط إجراء
+
+302
+00:22:26,210 --> 00:22:31,910
+الفحص، صايم؟ مش صايم؟ أكل إشي معين؟ متجنب أكل معين؟
+
+303
+00:22:31,910 --> 00:22:36,090
+ما أخد دواء؟ في فحوصات حكينالكم تجرى صباحا، في
+
+304
+00:22:36,090 --> 00:22:40,110
+فحوصات تجرى مساءً، في فحوصات تجرى بعد exercise،
+
+305
+00:22:40,110 --> 00:22:43,830
+بعد ما ياخد تمرين رياضة، بعد ما ياخد جرعة سكر أو
+
+306
+00:22:43,830 --> 00:22:47,390
+.. أو إشي معين، فبالتالي أنا لازم أتأكَّد إن هو
+
+307
+00:22:47,390 --> 00:22:51,330
+مستوفي الشروط لإجراء الفحص، بدي أسأله: صايم؟ طب
+
+308
+00:22:51,330 --> 00:22:56,490
+كم ساعة صمت؟ عشان أتأكَّد من متى لمتى؟ صمت؟ طب أكلت
+
+309
+00:22:56,490 --> 00:22:59,150
+إشي؟ طب إيش الأدوية اللي بتاخدها؟ كل هذه الأشياء
+
+310
+00:22:59,150 --> 00:23:03,570
+لازم أنا أتأكَّد منها، تبعا لنوع الفحص اللي مطلوب،
+
+311
+00:23:03,570 --> 00:23:07,550
+prepare the blood collection assembly، طبعا لازم
+
+312
+00:23:07,550 --> 00:23:10,910
+أحضر الأدوات تبعَتي، يعني أركّب ال syringe و ال
+
+313
+00:23:10,910 --> 00:23:14,570
+needle، و أتأكَّد إن ال syringe .. ال syringe يا بنات،
+
+314
+00:23:14,570 --> 00:23:19,630
+ال plunger تبعها بيكون hectares، فلازم ألَيّنه و أخلي
+
+315
+00:23:19,630 --> 00:23:24,000
+أتأكَّد من إنه بيروح و بيجي بسهولة، جاهز أجي أصحى
+
+316
+00:23:24,000 --> 00:23:31,460
+بالعينة، تمام؟ و طبعا بتأكَّد أنه ب .. ب .. بحرك ال
+
+317
+00:23:31,460 --> 00:23:34,080
+needle .. بركّب ال needle و بحركها بحيث أن ال bevel
+
+318
+00:23:34,080 --> 00:23:40,340
+تبعها، الفتحة تبعها، تكون مقابلة لإيش؟ للتدريج،
+
+319
+00:23:40,340 --> 00:23:43,400
+تكون الفتحة مقابلة للتدريج اللي موجود على ال
+
+320
+00:23:43,400 --> 00:23:49,780
+syringe، تمام؟ طيب، فإذا، إذا بركّب الأدوات تبعَتي، ال
+
+321
+00:23:49,780 --> 00:23:51,940
+needle is firmly adjusted to the tip of the
+
+322
+00:23:51,940 --> 00:23:55,140
+syringe، بركّب ال needle كويس على ال syringe، it
+
+323
+00:23:55,140 --> 00:23:58,360
+check is then made to see that the plunger is not
+
+324
+00:23:58,360 --> 00:24:02,000
+stick with the barrel، بتأكَّد أنه ال plunger مش
+
+325
+00:24:02,000 --> 00:24:07,700
+ملتصق بال barrel، يعني مش .. مش تارس، طبعا لأ، بالعكس، ألَيّن
+
+326
+00:24:07,700 --> 00:24:14,390
+بخليه، بحركه مرة، مرتين، تلاتة، بحيث أنه أتأكَّد إن هو
+
+327
+00:24:14,390 --> 00:24:19,470
+صار ليّن، بعدين طبعا بجلس ال patient، position ده،
+
+328
+00:24:19,470 --> 00:24:26,450
+patient، بجلسه على كرسي أو بخليه يستلقي و بيمدّ لي
+
+329
+00:24:26,450 --> 00:24:31,570
+إيده، تمام؟ و بعد هيك إيش بدي أعمل؟ Apply the tourniquet
+
+330
+00:24:31,570 --> 00:24:34,810
+from three to four inches above the selected
+
+331
+00:24:34,810 --> 00:24:39,630
+puncture site، مكان ما أنا بدي أسحب العينة، بروح بحط
+
+332
+00:24:39,630 --> 00:24:44,940
+ال tourniquet لفوقها، تمام؟ يعني باتجاه الكتف، لفوق،
+
+333
+00:24:44,940 --> 00:24:49,640
+من تلاتة لأربعة inches، طبعا، طب كيف هذي بدي أخدها؟
+
+334
+00:24:49,640 --> 00:24:54,920
+يعني أنا بحط، يعني فوق منطقة الكوع، بتلات لأربع أصابع،
+
+335
+00:24:54,920 --> 00:24:59,400
+بحط تلات أصابع من إيدي و بحدد المنطقة اللي أنا بدي
+
+336
+00:24:59,400 --> 00:25:01,400
+أربط فيها ال tourniquet، زي ما إنتوا شايفين ال
+
+337
+00:25:01,400 --> 00:25:05,300
+tourniquet هي عبارة عن رباط مطاطي أو حزام، بربط فيه
+
+338
+00:25:05,300 --> 00:25:10,080
+بحيث إنه يعملي ضغط على منطقة الكوع بحيث تبرز
+
+339
+00:25:10,080 --> 00:25:14,820
+منطقة ال anticubital، بحيث تبرز ال .. الأوردة، طبعا
+
+340
+00:25:14,820 --> 00:25:21,160
+don't place too tightly or leave on more than two
+
+341
+00:25:21,160 --> 00:25:25,040
+minutes، يعني مش لازم أنا أضغط، يعني أعملها حزْجة
+
+342
+00:25:25,040 --> 00:25:28,900
+كتير ال tourniquet، و بنفس الوقت مش لازم أنا أخليها
+
+343
+00:25:28,900 --> 00:25:33,500
+مربوطة أكتر من .. من دقيقتين، لأن ساعتها راح يصير
+
+344
+00:25:33,500 --> 00:25:37,780
+فيه تأثير على العينة، كمان شوية راح نحكي عنه، تمام؟
+
+345
+00:25:37,780 --> 00:25:43,360
+طيب، فبالتالي مش لازم، عشان هيك أنا بحضّر أدواتي،
+
+346
+00:25:43,360 --> 00:25:47,940
+تمام؟ Prepare the blood collection assembly، بعدين
+
+347
+00:25:47,940 --> 00:25:53,870
+بربط الرابطة، تمام؟ طيب، بعدين the patient should
+
+348
+00:25:53,870 --> 00:25:59,350
+make a fist without bumping the hand، بخليه ب .. ب
+
+349
+00:25:59,350 --> 00:26:02,810
+.. بحكي للمريض إنه يسكر إيده و يضغط على إيده، بس ما
+
+350
+00:26:02,810 --> 00:26:06,090
+ي .. ما .. ما يفتحهاش و يسكرها، without bumping، أنا
+
+351
+00:26:06,090 --> 00:26:09,670
+بدي إياه يظل يفتح و يسكر فيها، لأ، بس يسكرها و يضغط
+
+352
+00:26:09,670 --> 00:26:14,410
+عليها على سكّر إيده، تمام؟ عشان تبرز الأوردة، طبعا
+
+353
+00:26:14,410 --> 00:26:18,420
+يا بنات، ال .. ال .. الرابطة .. رابطة ال tourniquet
+
+354
+00:26:18,420 --> 00:26:22,600
+إلها طريقة بحيث إنها تكون ماخدة شكل لفّة، طبعا؟
+
+355
+00:26:22,600 --> 00:26:26,200
+على أساس إن أنا أسهل أفكها، إن شاء الله راح أعلمكم
+
+356
+00:26:26,200 --> 00:26:30,720
+إياها داخل المختبر، و زي ما حكيت لكم، راح أصوّر video
+
+357
+00:26:30,720 --> 00:26:37,350
+و هذا ال .. ال video راح أبث لكم إياه، بحيث إنه أو
+
+358
+00:26:37,350 --> 00:26:40,630
+يعني أرسل لكم إياه، بحيث إنه إنتوا تشوفوا كيف عملية
+
+359
+00:26:40,630 --> 00:26:44,750
+السحب، كيف أنا بربط الرابطة، كيف بحضّر الأدوات، كيف
+
+360
+00:26:44,750 --> 00:26:51,310
+بخلي ال plunger تبع ال tourniquet يصير سهل ليّن، و كيف
+
+361
+00:26:51,310 --> 00:26:57,230
+بحس الوريد، أي وريد بقَرِّر إن أنا آخده للسحب، و بعد هيك
+
+362
+00:26:57,230 --> 00:27:01,750
+طبعا، بعد ما أختار الوريد اللي بده سحب، طبعا بحسه من
+
+363
+00:27:01,750 --> 00:27:06,970
+خلال بإصبع السبابة، بعدين بعقّم، بحط على قطنة كحول و
+
+364
+00:27:06,970 --> 00:27:12,150
+بعقّم بشكل دائري، تمام؟ بعقّم بشكل دائري يا بنات، من
+
+365
+00:27:12,150 --> 00:27:16,930
+المركز و بأخذ، بأبعد على الأطراف، يعني ب .. يعني
+
+366
+00:27:16,930 --> 00:27:20,570
+دائرة ضيقة و ببدأ أوسّع، أوسّع، أوسّع، لِمن ما أصل
+
+367
+00:27:20,570 --> 00:27:26,020
+للأطراف، و هكذا، كيف يعني؟ لأنه أنا ساعتها بدي آخذ ال
+
+368
+00:27:26,020 --> 00:27:28,780
+contamination اللي موجودة في المركز، عند منطقة
+
+369
+00:27:28,780 --> 00:27:35,240
+الوريد و أُبعدها للأطراف، تمام؟ فإذا، بقت الحركة
+
+370
+00:27:35,240 --> 00:27:40,300
+دائرية من .. تتسع من المركز للأطراف، هاي حركة
+
+371
+00:27:40,300 --> 00:27:44,120
+التعقيم، أو ممكن تكون مسحة و خلاص، ما أرجعش أعيد تاني،
+
+372
+00:27:45,050 --> 00:27:48,450
+تمام؟ ليش .. ليش أنا ب .. بعملش العكس، بعملش من
+
+373
+00:27:48,450 --> 00:27:51,430
+الأطراف للمركز، ساعتها أنا بكون .. قاعدة بلمّ كل ال
+
+374
+00:27:51,430 --> 00:27:55,010
+.. ال contamination و بحطّ هوايا في منطقة السحب،
+
+375
+00:27:55,010 --> 00:28:00,800
+إذا بدي أنزح من الأطراف للمركز، تمام؟ لو بدي أعمل
+
+376
+00:28:00,800 --> 00:28:03,200
+مسحة و أرجع أعيد عليها، معناها أنا أخدت ال
+
+377
+00:28:03,200 --> 00:28:07,440
+contamination و رجعت عيدت عليها تاني، تمام؟ و رجعت
+
+378
+00:28:07,440 --> 00:28:12,120
+رجعتها مكانها، فهذا غلط، إذا بختار الوريد اللي أنا
+
+379
+00:28:12,120 --> 00:28:19,340
+بدي أسحب و بعقّم المكان بشكل دائري، و بعد هيك، طبعا
+
+380
+00:28:19,340 --> 00:28:24,080
+بخلي الكحول يجف، طبعا إذا أنا استخدمت كحول كتير،
+
+381
+00:28:24,080 --> 00:28:28,780
+لازم أجفّف وأنا بنفسي، ليش؟ يعني بدي أستخدم قطعة
+
+382
+00:28:28,780 --> 00:28:35,540
+قَطْنة كحول .. قطعة قطن معقّمة وأنشّف فيها الكحول، لكن لو
+
+383
+00:28:35,540 --> 00:28:40,520
+أنا بدي .. طب استخدام الكحول كمية معقولة، هو على
+
+384
+00:28:40,520 --> 00:28:43,880
+طول راح يتبخر و يِعدي، طب ليش أنا بدي أجفّفه بنفسي؟
+
+385
+00:28:43,880 --> 00:28:47,400
+الحكاية، عشان ما يتبخر و ياخد معاه ماء، و بالتالي يصير
+
+386
+00:28:47
+
+445
+00:33:26,540 --> 00:33:30,560
+يا بنات، ليش أنا بلمّ .. ب .. بحسّ اتجاه الوريد؟
+
+446
+00:33:30,560 --> 00:33:35,060
+يعني ب .. ألمسه بالسبابة و بحاول أعرف اتجاهه؟ عشان
+
+447
+00:33:35,060 --> 00:33:38,560
+أنا لما بدي أفوت في الإبرة، أفوت و أنا في .. بكون
+
+448
+00:33:38,560 --> 00:33:42,420
+في نفس اتجاه المريض، في اتجاه الوريد، sorry، في نفس
+
+449
+00:33:42,420 --> 00:33:46,420
+اتجاه الوريد، لو الوريد هيك جاي، بروح بعكس اتجاه
+
+450
+00:33:46,420 --> 00:33:51,260
+الإبرة تبعتي بحيث أن أنا أجيله هيك، تمام؟ طيب، هي
+
+451
+00:33:51,260 --> 00:33:54,280
+الوريد .. لكن لو شايفين هنا هدي ال .. ال .. ال
+
+452
+00:33:54,280 --> 00:33:58,430
+needle مش في اتجاه الوريد، جاية معاكسها، معاها مدى
+
+453
+00:33:58,430 --> 00:34:02,290
+ضيق، إنها تفوت داخل الوريد، طبعا؟ معاها مدى ضيق،
+
+454
+00:34:02,290 --> 00:34:05,090
+يعني ممكن إنها تيجي تخترق الوريد و تفوت من الجهة
+
+455
+00:34:05,090 --> 00:34:07,510
+التانية، تخترق الوريد من الجهة التانية، ساعتها الدم
+
+456
+00:34:07,510 --> 00:34:10,810
+الذي يصير يسرب من الجهة التانية و يتجمع تحت
+
+457
+00:34:10,810 --> 00:34:15,470
+الدم، تحت الجلد، تحت الوريد، وهذا مشكلة، هلاجي
+
+458
+00:34:15,470 --> 00:34:20,180
+إيد المريض، أنتِ فخْدِ غلط، لازم أنا أفوت في اتجاه
+
+459
+00:34:20,180 --> 00:34:23,460
+الوريد، معايا الـ needle، شايفين؟ طول و أنا فايتة، لو
+
+460
+00:34:23,460 --> 00:34:27,260
+فوتت أكثر، لو فوتت أكثر، بلاحظ أنه إيش أنا معايا
+
+461
+00:34:27,260 --> 00:34:33,440
+اتجاهها أكثر لحتى إني أفوت فيه، فبالتالي يعني بقلّل
+
+462
+00:34:33,440 --> 00:34:36,900
+من خطر أنّه يحدث عند المريض انتفاخ في إيده أو تسرب
+
+463
+00:34:36,900 --> 00:34:41,760
+في الدم تحت الـ skin، المعروف بـ hematoma، تمام؟
+
+464
+00:34:44,030 --> 00:34:48,750
+بُحضّر الأدوات تبعتي، بربط الـ tourniquet، بحدّد الوريد،
+
+465
+00:34:48,750 --> 00:34:53,290
+بعقّم تماما الوريد، طبعا في عندي مسكة للـ syringe،
+
+466
+00:34:53,290 --> 00:34:56,350
+شايفين؟ شايفين؟ الـ .. الـ .. الـ syringe و الـ needle،
+
+467
+00:34:56,350 --> 00:35:00,590
+السبابة بتكون عند الـ hub، و شايفين بإيدي الإبهام،
+
+468
+00:35:00,590 --> 00:35:05,930
+أنا شادة الـ skin بحيث أنه يبرز الوريد، و بمسك الـ ..
+
+469
+00:35:05,930 --> 00:35:09,530
+ب .. باقي ال .. الأصابع كلها هيك، شايفينها كلها
+
+470
+00:35:09,630 --> 00:35:12,690
+بتكون موجودة تحت الـ syringe، و الإبهام هذا بيكون
+
+471
+00:35:12,690 --> 00:35:15,710
+موجود فوق الـ syringe، حأورّجيكم إياها إن شاء الله
+
+472
+00:35:15,710 --> 00:35:20,330
+حسَوِّرْكُم في الـ video، حتشوفوا بعدين، بفوت في اتجاه
+
+473
+00:35:20,330 --> 00:35:24,550
+الوريد في زاوية معينة، من خمسة عشر إلى ثلاثين، لو كان يا
+
+474
+00:35:24,550 --> 00:35:29,130
+بنت، الوريد deep، بكبّر الزاوية، لو كان الوريد
+
+475
+00:35:29,130 --> 00:35:33,130
+superficial، بُصَغِّر الزاوية، تمام؟ مهم جدا هذه الشغلة،
+
+476
+00:35:33,130 --> 00:35:37,370
+و بعد هيك، مجرد ما أفوت في الوريد و أتعلم، عندي هنا
+
+477
+00:35:37,370 --> 00:35:43,090
+قطرة دم، ببدأ أرجع الـ plunger لورى، بسحب بيدي
+
+478
+00:35:43,090 --> 00:35:48,230
+التانية الـ plunger، و ببدأ يرجع عندي الـ blood في الـ
+
+479
+00:35:48,230 --> 00:35:52,540
+syringe، مجرد ما خلصت، أخدت الحجم اللي بدّي إياه، أول إيش
+
+480
+00:35:52,540 --> 00:35:55,640
+بعمله، بربط ب .. ب .. ب .. ب .. ب .. ب .. ب .. ب ..
+
+481
+00:35:55,640 --> 00:35:55,880
+ب .. ب .. ب .. ب .. ب .. ب .. ب .. ب .. ب .. ب ..
+
+482
+00:35:55,880 --> 00:35:58,300
+ب .. ب .. ب .. ب .. ب .. ب .. ب .. ب .. ب .. ب ..
+
+483
+00:35:58,300 --> 00:35:58,780
+ب .. ب .. ب .. ب .. ب .. ب .. ب .. ب .. ب .. ب ..
+
+484
+00:35:58,780 --> 00:35:58,800
+ب .. ب .. ب .. ب .. ب .. ب .. ب .. ب .. ب .. ب ..
+
+485
+00:35:58,800 --> 00:35:59,100
+ب .. ب .. ب .. ب .. ب .. ب .. ب .. ب .. ب .. ب ..
+
+486
+00:35:59,100 --> 00:35:59,860
+ب .. ب .. ب .. ب .. ب .. ب .. ب .. ب .. ب .. ب ..
+
+487
+00:35:59,860 --> 00:36:00,800
+ب .. ب .. ب .. ب .. ب .. ب .. ب .. ب .. ب .. ب ..
+
+488
+00:36:00,800 --> 00:36:11,140
+ب .. ب .. ب .. ب .. ب .. ب .. ب .. ب ..
+
+489
+00:36:18,780 --> 00:36:22,560
+تمام؟ علشان لو أنا عملت العكس، لو طلعت من الوريد،
+
+490
+00:36:22,560 --> 00:36:26,400
+و لسه فيه ضغط، لسه فيه الـ .. الـ tourniquet مربوطة،
+
+491
+00:36:26,400 --> 00:36:31,560
+راح الدم .. الإجْتِماع، يندفع إِلى خارج الـ
+
+492
+00:36:31,560 --> 00:36:34,680
+skin، راح يصير في زي الـ bleeding، و جزء منه كمان
+
+493
+00:36:34,680 --> 00:36:37,460
+حَيُعْبِي تحت الـ skin و يعملي انتفاخ، يعني هيعملي
+
+494
+00:36:37,460 --> 00:36:41,900
+hematoma، تمام؟ هذه شغلة، الشغلة التانية هنا، الترتيب
+
+495
+00:36:41,900 --> 00:36:45,860
+أيضا، أن أنا أطلع الـ needle بعدين أحط القطنة، تخيّل
+
+496
+00:36:45,860 --> 00:36:50,520
+لو أنا الـ needle جوا، حطّيت القطنة و ضغطت، يعني أنا
+
+497
+00:36:50,520 --> 00:36:53,200
+ضغطت على الـ needle اللي هو جوا، إذن أنا سبّبت ألم
+
+498
+00:36:53,200 --> 00:36:56,980
+للمريض و هي الإبرة جواته، ضغطت عليها، فغلط، لازم
+
+499
+00:36:56,980 --> 00:37:02,740
+أطلعها بعدين أحط القطنة و أخليه يضغط، تمام؟ خلينا نشوف
+
+500
+00:37:02,740 --> 00:37:08,800
+الـ .. الـ discussion، يعني المناقشة أو النقاط المهمة
+
+501
+00:37:08,800 --> 00:37:15,700
+المتعلقة بعملية جمع عينة الدم من الـ vein، تمام؟ الـ
+
+502
+00:37:15,700 --> 00:37:20,180
+venipuncture، أول شيء، there are certain areas to
+
+503
+00:37:20,180 --> 00:37:23,120
+be avoided when choosing a site for venipuncture،
+
+504
+00:37:23,120 --> 00:37:26,860
+يعني أنا لما ببدأ أختار مكان الوريد، بدّي أبعد عن هاي
+
+505
+00:37:26,860 --> 00:37:30,920
+الشغلات، أول شيء طبعا هذا أيضا بيكون موضوع السؤال،
+
+506
+00:37:30,920 --> 00:37:37,960
+extensive scars، يعني لو كان فيه ندوب أو حروق أو أثر
+
+507
+00:37:37,960 --> 00:37:42,380
+جراحة، أثر جروح موجود في مكان الوريد، بأبعد عنه،
+
+508
+00:37:42,380 --> 00:37:47,140
+تمام؟ و أيضا إذا كان في الـ .. في الإيد المحاذية،
+
+509
+00:37:47,140 --> 00:37:54,030
+لما كان صار له mastectomy، يعني مثلا في الأماكن،
+
+510
+00:37:54,030 --> 00:38:00,850
+اللي، يعني لو الست صار عندها استئصال لثدي مثلا،
+
+511
+00:38:00,850 --> 00:38:05,170
+الثدي الأيمن، ممنوع أنا أسحب من الإيد اليمنى، الإيد
+
+512
+00:38:05,170 --> 00:38:07,610
+المحاذية للمكان اللي صار فيه mastectomy، صار
+
+513
+00:38:07,610 --> 00:38:13,610
+الاستئصال، للكتلة، ممنوع أسحب منها، تمام؟ عشان هيك
+
+514
+00:38:13,610 --> 00:38:16,890
+بتلاحظوا أن مرضى الـ .. الـ .. الـ breast cancer،
+
+515
+00:38:16,890 --> 00:38:22,330
+الناس اللي تعرضوا لاستئصال، بيلبسوا دائما إسورة في
+
+516
+00:38:22,330 --> 00:38:26,630
+إيدهم المحاذية لمنطقة الاستئصال، و مكتوب على هذه
+
+517
+00:38:26,630 --> 00:38:31,280
+الإسورة، "don't touch my arm"، تمام؟ يعني لا تلمس إيدي،
+
+518
+00:38:31,280 --> 00:38:34,340
+عشان هدول الناس، لو تعرضوا لإغماء أو لو نقلوا إلى
+
+519
+00:38:34,340 --> 00:38:37,560
+المستشفى، أو لو كانوا مش بوعيهم، و إجينا بنسحب منهم
+
+520
+00:38:37,560 --> 00:38:41,220
+عينة، مجرد بنشوف هذه الإسورة اللي مكتوب عليها "don't
+
+521
+00:38:41,220 --> 00:38:44,360
+touch my arm"، راح أعرف إنه في هذه المنطقة أو في
+
+522
+00:38:44,360 --> 00:38:49,480
+المنطقة المحاذية، صار في mastectomy، وبالتالي مش
+
+523
+00:38:49,480 --> 00:38:52,830
+ممنوع، أنا أسحب منها عينة دم، طب لو سحبت عينة الدم، إيش
+
+524
+00:38:52,830 --> 00:38:57,290
+بيصير؟ بيصير في عند المريض lymphedema، بتنتفخ الغدد
+
+525
+00:38:57,290 --> 00:39:01,670
+اللمفاوية عنده، بيصير في عنده آلام كبيرة، تمام؟ و
+
+526
+00:39:01,670 --> 00:39:06,910
+أيضا، المنطقة اللي فيها hematoma، لو شخص من .. يعني
+
+527
+00:39:06,910 --> 00:39:11,390
+انسحب له عينة دم قبلًا و صار في عنده hematoma، تمام؟
+
+528
+00:39:11,390 --> 00:39:16,500
+يعني انتفاخ في منطقة السحب نتيجة تراكم الدم تحت الـ
+
+529
+00:39:16,500 --> 00:39:20,400
+skin، هذا برضه كمان، بتجنّب أن أنا أسحب له من منطقة الـ
+
+530
+00:39:20,400 --> 00:39:25,580
+hematoma، لإنه هسحب دم مكسر، هسحب دم مكسر، ممنوع أن
+
+531
+00:39:25,580 --> 00:39:29,300
+أنا أسحب في المنطقة هذه، بسحب بعدها أو قبلها أو من
+
+532
+00:39:29,300 --> 00:39:32,640
+الإيد التانية، بستنى عليها، للطّيب، الـ hematoma يا
+
+533
+00:39:32,640 --> 00:39:35,620
+بنات، بالطّيب، يعني بتاخد لها فترة من أسبوع لعشرة أيام،
+
+534
+00:39:35,620 --> 00:39:42,150
+و بتروح، يعني الدم يعني، يترَكّب تحت الـ skin، الجسم بيتخلّص
+
+535
+00:39:42,150 --> 00:39:47,140
+منه بعد فترة، تمام؟ طيب، في .. أيضا في منطقة الـ
+
+536
+00:39:47,140 --> 00:39:49,900
+intravenous therapy، لو الشخص بياخد blood
+
+537
+00:39:49,900 --> 00:39:55,840
+transfusion، عمّ ننقله دم بالوريد أو عمّ ننقله سائل
+
+538
+00:39:55,840 --> 00:39:59,980
+محلول، فبالتالي، ساعتها، هيكون الدم، إذا بننقله
+
+539
+00:39:59,980 --> 00:40:03,940
+محلول، يعني الدم مخفّف في هاي المنطقة، تمام؟ إذا عمّ
+
+540
+00:40:03,940 --> 00:40:07,780
+ننقله دم، يعني وحدة دم، معناه الدم اللي في المنطقة
+
+541
+00:40:07,780 --> 00:40:12,030
+هذه مش دمه لسه، تمام؟ هذا الدم اللي انْتُقِل، فلازم
+
+542
+00:40:12,030 --> 00:40:16,330
+يصير فيه circulation، علشان أنا ا .. يختلط الدم في
+
+543
+00:40:16,330 --> 00:40:21,730
+بعض، و .. و يصير يعني العينة معبرة، فبالتالي .. طب ..
+
+544
+00:40:21,730 --> 00:40:24,910
+فبالحالة هذه، أنا المنطقة هذه مش لازم أسحب منها،
+
+545
+00:40:24,910 --> 00:40:30,890
+المنطقة اللي فيها cannula لمحلول أو لوحـة نقل، وحتى
+
+546
+00:40:30,890 --> 00:40:35,690
+الدم، طب إيش بدّي أعمل لو كان مثلا الشخص هذا، يا إما
+
+547
+00:40:35,690 --> 00:40:39,850
+بنجي ل .. ب .. بسحب له من الإيد التانية، أو إيش بعمل
+
+548
+00:40:39,850 --> 00:40:47,930
+لو الشخص هذا عم بياخد سائل؟ محلول وريدي،
+
+549
+00:40:47,930 --> 00:40:54,510
+أول شيء، turn off the IV، بسكّر الـ .. الـ flow، بسكّر الـ
+
+550
+00:40:54,510 --> 00:40:58,290
+cannula، بحيث أنه ما يفوت له IV لمدة دقيقتين على
+
+551
+00:40:58,290 --> 00:41:03,090
+الأقل، قبل الـ venipuncture، تمام؟ و بحط الـ tourniquet
+
+552
+00:41:03,090 --> 00:41:08,710
+تحت مكان الـ IV site، مكان ما هو داخل الـ cannula، و
+
+553
+00:41:08,710 --> 00:41:13,010
+مكان ما دخل المحلول، أنا بربط الـ tourniquet تحتها،
+
+554
+00:41:13,010 --> 00:41:17,430
+ليش؟ لإنه يا بنات، خليني مثلا أشرح لكم على الصورة،
+
+555
+00:41:17,430 --> 00:41:22,910
+هنا، على صورة الأوردة، نرجع هنا، نفرض إنه هنا في
+
+556
+00:41:22,910 --> 00:41:26,470
+cannula، تمام؟ بسكّر أول شيء الـ cannula، بحيث إنه
+
+557
+00:41:26,470 --> 00:41:33,330
+يتوقف الـ IV، و تاني شغلة يا بنات إيش بعمل؟ بركب الـ
+
+558
+00:41:33,330 --> 00:41:37,150
+tourniquet تحت، هلّأ المحلول هو رايح فوق، هيك
+
+559
+00:41:37,150 --> 00:41:41,150
+رايح، في هذا الاتجاه، تمام؟ فالدم اللي هنا مش
+
+560
+00:41:41,150 --> 00:41:44,170
+مختلط بالمحلول، عشان هيك أنا بحط الـ tourniquet تحت،
+
+561
+00:41:44,170 --> 00:41:49,070
+و بسحب من الأوردة اللي تحت، مكان الـ IV، تمام؟
+
+562
+00:41:49,070 --> 00:41:54,810
+هاي معنى كلمة "apply the tourniquet below the IV"،
+
+563
+00:41:54,810 --> 00:42:00,090
+site، select a vein، بدوّر على vein كويس، وريد منيح، و
+
+564
+00:42:00,090 --> 00:42:05,850
+مناسب لسحب العينة، و بأجري الـ venipuncture، بسحب
+
+565
+00:42:05,850 --> 00:42:10,250
+أول خمسة ملي، و بتخلص منهم، بعدين بسحب العينة اللي
+
+566
+00:42:10,250 --> 00:42:15,090
+بدّي إياها، كمان كل هذه إجراءات بعملها علشان أتأكّد أن
+
+567
+00:42:15,090 --> 00:42:19,990
+أنا بأخذ عينة ممثلة، representative sample، عينة لما
+
+568
+00:42:19,990 --> 00:42:23,830
+أحصل، يعني لما أعمل عليها تحاليل، أحصل على نتائج
+
+569
+00:42:23,830 --> 00:42:29,660
+تكون النتائج معبرة عن وضع المريض، تمام؟ hematoma، إيش
+
+570
+00:42:29,660 --> 00:42:33,780
+يعني hematoma؟ حكينا هو انتفاخ في منطقة السحب،
+
+571
+00:42:33,780 --> 00:42:40,380
+تمام؟ نتيجة إيش؟ نتيجة أن blood accumulates ..
+
+572
+00:42:40,380 --> 00:42:46,260
+blood accumulates under the skin، تمام؟ ف .. هي تحدث
+
+573
+00:42:46,260 --> 00:42:49,900
+from under the skin adjacent to the puncture site،
+
+574
+00:42:49,900 --> 00:42:54,280
+تحدث تحت الـ skin، في منطقة إيش؟ الـ .. الـ .. السحب،
+
+575
+00:42:54,800 --> 00:42:58,160
+release، طبعا، إذا أول ما أشوفها، أول ما أشوف أنه صار
+
+576
+00:42:58,160 --> 00:43:01,100
+فيه انتفاخ في هذه المنطقة، يعني الدم اتجمع في هذه
+
+577
+00:43:01,100 --> 00:43:04,360
+المنطقة، أول شيء بعمله، release the tourniquet
+
+578
+00:43:04,360 --> 00:43:08,320
+immediately، برفع الـ tourniquet immediately، و بسحب
+
+579
+00:43:08,320 --> 00:43:13,020
+الـ needle، و بخلي المريض يحط قطنة و يضغط عليها، طب
+
+580
+00:43:13,020 --> 00:43:16,060
+إيش أسباب الـ hematoma؟ إيش اللي بيخلي الدم يتجمع
+
+581
+00:43:16,060 --> 00:43:21,800
+تحت الـ skin؟ هنا يطلع من الوريد و يفوت داخل الـ skin،
+
+582
+00:43:23,000 --> 00:43:26,920
+يعني يتجمع ما بين .. تحت الـ skin، أول شيء، the
+
+583
+00:43:26,920 --> 00:43:29,780
+needle may have gone through the vein، يعني الـ ..
+
+584
+00:43:29,780 --> 00:43:33,700
+الـ .. الـ .. الـ needle اخترقت الوريد، هاي الوريد
+
+585
+00:43:33,700 --> 00:43:36,640
+اللي لونه أحمر، فاتت فيه و طلعت من الجهة التانية،
+
+586
+00:43:36,640 --> 00:43:38,800
+يعني أنتِ فاقِعْتِه من الجهة التانية، الجهة التانية
+
+587
+00:43:38,800 --> 00:43:42,440
+هدا، راح تصير تمزف و يتجمع الدم تحت الـ skin، إذا
+
+588
+00:43:42,440 --> 00:43:46,800
+سَيْـ .. راح يصير انتفاخ زي هيك، remove the needle
+
+589
+00:43:46,800 --> 00:43:50,440
+before removing the tourniquet، لو أنا رفعت الـ
+
+590
+00:43:50,440 --> 00:43:53,760
+needle قبل الـ tourniquet، حكينا حيصير أول شيء
+
+591
+00:43:53,760 --> 00:43:58,760
+bleeding، و جزء من الدم حيتحبس تحت الـ skin، فهيصير في
+
+592
+00:43:58,760 --> 00:44:03,700
+عندي hematoma، تمام؟ عشان هيك حكينا الترتيب مهم،
+
+593
+00:44:03,700 --> 00:44:07,030
+برفع الـ tourniquet بالأول، بعدين الـ needle، the
+
+594
+00:44:07,030 --> 00:44:12,130
+needle may not fully penetrate the uppermost wall
+
+595
+00:44:12,130 --> 00:44:18,330
+of the vein، يعني لو أنا الـ .. الـ .. الـ needle لو كانت
+
+596
+00:44:18,330 --> 00:44:24,070
+يعني زي ما أنتم شايفين هنا، مش مخترقة بشكل كامل،
+
+597
+00:44:24,070 --> 00:44:27,210
+الوريد، هذا الـ bevel نصّه جاي .. هاي الـ bevel،
+
+598
+00:44:27,210 --> 00:44:31,590
+شايفينه؟ الجزء المائل هذا اللي بزاوية، جاي فتحة الـ
+
+599
+00:44:31,590 --> 00:44:37,250
+needle، طبعا جزء هذا الـ bevel، زي ما أنتم شايفين، جزء
+
+600
+00:44:37,250 --> 00:44:39,910
+من الـ bevel هذا مخترق الوريد، والجزء التاني برا،
+
+601
+00:44:39,910 --> 00:44:43,130
+يعني هلّأ الدم اللي في الوريد، بيجي بِعْبِي في الـ bevel،
+
+602
+00:44:43,130 --> 00:44:47,670
+بيطلع من الجزء اللي برا، و بيتحوّش تحت الـ skin، يعني
+
+603
+00:44:47,670 --> 00:44:50,770
+بتكون تحت الـ skin هنا، accumulates under the skin،
+
+604
+00:44:50,770 --> 00:44:56,970
+و بيعمل لي انتفاخ، إذا hematoma، تمام؟ فهذا أيضا أحد
+
+605
+00:44:56,970 --> 00:45:03,090
+الأسباب، و أيضا، failure to apply pressure، إذا أنا
+
+606
+00:45:03,090 --> 00:45:06,690
+حكيت للمريض اضغط بعد ما خلصت، إذا أنا سحبت بطريقة
+
+6
+
+667
+00:49:34,810 --> 00:49:40,110
+الإيد أكثر من two minutes، تمام؟ يعني مانع اختلاط
+
+668
+00:49:40,110 --> 00:49:44,330
+الدم اللي موجود في منطقة السحب مع باقي الدم اللي
+
+669
+00:49:44,330 --> 00:49:47,330
+موجود في الـ circulation طالما أنا منعت اختلاطه
+
+670
+00:49:47,330 --> 00:49:50,790
+يعني الدم هذا أتركز صار .. صار فيه
+
+671
+00:49:50,790 --> 00:49:55,560
+hemoconcentration، فبالتالي يعني أن كل شيء .. كل
+
+672
+00:49:55,560 --> 00:49:59,940
+شيء صار له increased concentration، تمام؟ إذا الـ
+
+673
+00:49:59,940 --> 00:50:03,780
+hemoconcentration هي تعريفها يأتي بالمصطلح is
+
+674
+00:50:03,780 --> 00:50:07,000
+defined as increased concentration of larger
+
+675
+00:50:07,000 --> 00:50:11,000
+molecules and formed elements in the blood or non-
+
+676
+00:50:11,000 --> 00:50:13,260
+filtrable elements، تمام؟
+
+677
+00:50:17,100 --> 00:50:21,340
+طبعاً أيضاً، كمان أني أربط الـ tourniquet أكثر من
+
+678
+00:50:21,340 --> 00:50:25,040
+two minutes، حكينا لما آجي أسحب ممكن ينقبض الوريد
+
+679
+00:50:25,040 --> 00:50:29,440
+يصير فيه vein collapse، ويتوقف السحب، يعني بأصحب شوية
+
+680
+00:50:29,440 --> 00:50:32,340
+دم بلاجي وجافقاش فجأة، أه ممكن أنا أكون
+
+681
+00:50:32,340 --> 00:50:36,080
+طولت على الـ tourniquet، تشوف الـ كيميا هلاحظ أن صار
+
+682
+00:50:36,080 --> 00:50:38,800
+فيه ازرقاق في المنطقة نتيجة أن الـ oxygen delivery
+
+683
+00:50:38,800 --> 00:50:42,360
+أو الـ oxygen قل في المنطقة هذه لأن أنا محبسة، يعني
+
+684
+00:50:42,360 --> 00:50:46,580
+أنا ضاغطة، تخيل أنت شخص .. يعني أحياناً الطفل لما
+
+685
+00:50:46,580 --> 00:50:51,380
+بيجي بيكون لف على إيده خيط أو مغيطة أو ضاغط عليها
+
+686
+00:50:51,380 --> 00:50:55,500
+بتحس أن المنطقة ازرقت نتيجة أن هو إيش؟ اتحبس الدم
+
+687
+00:50:55,500 --> 00:50:59,050
+فهي نفس الفكرة، هذه معناها كلمة tissue ischemia، يعني
+
+688
+00:50:59,050 --> 00:51:03,830
+ازرقاق ونقص في الـ oxygen، تمام؟ air must never be
+
+689
+00:51:03,830 --> 00:51:07,950
+pumped into a vein because a few cubic centimeters
+
+690
+00:51:07,950 --> 00:51:13,260
+of air pumped into a vein could cause death، لازم أنا
+
+691
+00:51:13,260 --> 00:51:19,460
+أتأكد أن الـ syringe مسكرة أثناء سحب العينة، يعني
+
+692
+00:51:19,460 --> 00:51:23,440
+أنا صح بلينها، بحرك البلانجر مرة مرتين تلات عشان
+
+693
+00:51:23,440 --> 00:51:28,020
+ألينها، بعدين بسكرها، يعني بفرغها من الهواء قبل ما أبدأ في السحب، لأن إذا أنا حقنت هواء في الوريد هذا
+
+694
+00:51:28,020 --> 00:51:31,320
+قاتل، تمام؟ ممنوع، plunger of syringe mustn't be
+
+695
+00:51:37,590 --> 00:51:41,710
+pulled back with too much force، لما أنا .. أنا .. لما
+
+696
+00:51:41,710 --> 00:51:45,830
+أفوت في الوريد ممنوع أ .. أرجع الـ plunger بقوة تبع
+
+697
+00:51:45,830 --> 00:51:49,930
+الـ syringe، ليش؟ أول حاجة حكينا بيسبب hemolysis أو
+
+698
+00:51:49,930 --> 00:51:54,150
+ممكن أن يسبب vein collapse، حكينا كمان طبعاً؟
+
+699
+00:51:54,240 --> 00:52:00,780
+ممكن أن أيضاً الجدار تبع الوريد يلتصق بالـ bevel، و
+
+700
+00:52:00,780 --> 00:52:05,220
+يتوقف الدم، إذا vein collapse، void، ممكن يصير فيه
+
+701
+00:52:05,220 --> 00:52:09,860
+hemolysis، ممكن أن الـ wall of vein، جدار الوريد
+
+702
+00:52:09,860 --> 00:52:16,060
+يعني ينسحب، يصير له pull أو يصير له الالتصاق adhere to
+
+703
+00:52:16,060 --> 00:52:21,650
+the bevel of needle، فبالتالي يتوقف سحب الدم، طيب
+
+704
+00:52:21,650 --> 00:52:26,230
+لو أنا في .. لو أنا بدأت أسحب دم وتوقف الدم فجأة
+
+705
+00:52:26,230 --> 00:52:29,630
+يعني أنا طلعت شوية دم بس مش الكمية الكافية، توقف
+
+706
+00:52:29,630 --> 00:52:32,790
+السحب، صارت ب .. برجع بالبلانجر ما عم بيعطيني الـ ..
+
+707
+00:52:32,790 --> 00:52:38,710
+يعني more blood، تمام؟ إيش بدي أعمل ساعتها؟ أول شيء
+
+708
+00:52:38,710 --> 00:52:42,950
+بدي أحرك، ممكن أنا أكون يعني اتحركت معايا الـ needle
+
+709
+00:52:42,950 --> 00:52:46,910
+والـ syringe، يعني فوتت بعمق أكثر أو غيرت الزاوية
+
+710
+00:52:46,910 --> 00:52:51,990
+أو غيرت الاتجاه، تمام؟ فساعتها أنا بدي أظبط الـ ..
+
+711
+00:52:51,990 --> 00:52:55,550
+الـ .. الـ position تبع الـ needle، يعني بدي أحركها
+
+712
+00:52:55,550 --> 00:53:01,290
+إما للأمام، تمام؟ أو أرجعها للخلف، وفي كل مرة أجرب
+
+713
+00:53:01,290 --> 00:53:04,950
+أن أرجع الـ plunger أشوف هو هيرجع يسحب ولا لا،
+
+714
+00:53:04,950 --> 00:53:11,010
+بكبر الزاوية، ب .. ب .. ب .. أصغرها، بشوف يعني بـ
+
+715
+00:53:11,010 --> 00:53:15,190
+.. ب .. ب .. بلفها يمين، بلفها يسار، بحيث أن يعني
+
+716
+00:53:15,190 --> 00:53:18,790
+أظبط أيضاً الاتجاه، إذا أول حاجة change the
+
+717
+00:53:18,790 --> 00:53:22,470
+position of the needle، move it forward، بحركها
+
+718
+00:53:22,470 --> 00:53:28,390
+للأمام or move it backward، أو للخلف، أسحبها للخلف
+
+719
+00:53:28,390 --> 00:53:32,550
+يعني أطلعها شوي، بحيث أن ممكن، ممكن أنا اخترقت
+
+720
+00:53:32,550 --> 00:53:38,050
+زيادة عن الـ zone، ممكن أكون adjust the angle، أظبط
+
+721
+00:53:38,050 --> 00:53:41,190
+الزاوية، أرفع الزاوية أو يعني أكبر الزاوية أو
+
+722
+00:53:41,190 --> 00:53:45,650
+أصغرها، ممكن يكون صار في عندي vein collapse، فالأفضل
+
+723
+00:53:45,650 --> 00:53:51,590
+أن أنا أعمل، أفتح يعني أفك الـ tourniquet، تمام، و ..
+
+724
+00:53:51,590 --> 00:53:54,950
+أو ممكن أرجع أدور على الوريد مرة ثانية عشان أشوف
+
+725
+00:53:54,950 --> 00:53:59,710
+وين اتجاهه وأدخله من خلال نفس الاتجاه، هذه الشغلات
+
+726
+00:53:59,710 --> 00:54:05,650
+كلها هتلاحظوا أنه .. هتلاحظوني بعلمكم إياها أثناء
+
+727
+00:54:05,650 --> 00:54:11,260
+الـ .. الممارسة العملية، لما مثلاً أنت تجربي مع زميلتك
+
+728
+00:54:11,260 --> 00:54:15,800
+وت .. تفاجئي أن .. أن سحبتي منها شوية blood
+
+729
+00:54:15,800 --> 00:54:19,740
+بعدين توقف الدم، إيش المشكلة؟ بصير أهاجي جنبك و
+
+730
+00:54:19,740 --> 00:54:24,920
+أحكي لك، ارجعيها لورا شوية، جربي، ما سحبتش، بحكيلك
+
+731
+00:54:24,920 --> 00:54:28,840
+طب دخليها لأكثر شوية، جربي، ما سحبتش، طب ارفعي الزاوية
+
+732
+00:54:28,840 --> 00:54:36,640
+طب قليليها، فكي الـ tourniquet وهكذا، طيب في blood stop
+
+733
+00:54:36,640 --> 00:54:44,020
+flowing، إذا الدم توقف تماماً أثناء يعني الـ syringe
+
+734
+00:54:44,020 --> 00:54:47,220
+فمعناها .. يعني أثناء الـ syringe في الـ syringe
+
+735
+00:54:47,220 --> 00:54:50,920
+فمعناها ممكن يكون أحد الأسباب غير أن أنا أغير
+
+736
+00:54:50,920 --> 00:54:54,420
+الموضوع، ممكن يكون صار فيه vein collapse، فبدي أنا
+
+737
+00:54:54,420 --> 00:54:57,140
+أرفع الـ tourniquet، لأن ممكن .. حكينا أن الـ
+
+738
+00:54:57,140 --> 00:55:01,240
+tourniquet إذا طولت ممكن تسبب لي أيضاً .. إيش ..
+
+739
+00:55:01,240 --> 00:55:07,620
+vein collapse، فبالتالي .. فإني أرفع الـ tourniquet
+
+740
+00:55:07,620 --> 00:55:11,820
+يعني أنا رفعت الضغط عن الوريد، فصار فيه سريان في ..
+
+741
+00:55:11,820 --> 00:55:17,340
+لـ .. للـ blood داخل الوريد، فبالتالي ممكن يرجع يتكو
+
+742
+00:55:17,340 --> 00:55:21,400
+.. يطلع عند blood، يرجع يصير الـ flow مرة ثانية، يطلع
+
+743
+00:55:21,400 --> 00:55:27,660
+في السرنج، يعبأ في السرنج، إذا ما نجحتش في أن أنا
+
+744
+00:55:27,660 --> 00:55:32,780
+أرجع سريان الدم داخل الـ syringe، ب .. برفع الـ needle
+
+745
+00:55:32,780 --> 00:55:38,080
+بطلعها، وبحط يعني بفك الـ tourniquet وبطلع الـ
+
+746
+00:55:38,080 --> 00:55:43,020
+needle وبحط قطنة وبخليه أن to apply pressure، و
+
+747
+00:55:43,020 --> 00:55:47,380
+بشوف مكان ثاني أني أسحب منه volume آخر من الدم
+
+748
+00:55:47,380 --> 00:55:50,980
+من اليد الثانية أو من وريد آخر في نفس اليد، the
+
+749
+00:55:50,980 --> 00:55:56,130
+needle may have pulled out of the vein، أحياناً طبعاً
+
+750
+00:55:56,130 --> 00:56:00,530
+يعني أن الدم، حكينا أن إذا توقف، إما كان صار له
+
+751
+00:56:00,530 --> 00:56:04,870
+collapse الوريد أو أن الـ needle اتحركت، وساعتها
+
+752
+00:56:04,870 --> 00:56:07,970
+أن أنا بدي أركز، بدي أرجع الـ .. الـ needle مكانها
+
+753
+00:56:07,970 --> 00:56:11,750
+مرة ثانية، إما بـ .. بالحركة للأمام أو للخلف أو أظبط
+
+754
+00:56:11,750 --> 00:56:17,690
+الزاوية أو أفك الـ tourniquet، تمام؟ هيك بنكون خلصنا
+
+755
+00:56:17,690 --> 00:56:22,230
+الجزئية المتعلقة بالـ vein puncture أو الـ phlebotomy، و
+
+756
+00:56:22,230 --> 00:56:25,230
+حكينا أن الـ phlebotomy هي نفسها الـ vein puncture، يعني
+
+757
+00:56:25,230 --> 00:56:31,190
+الحصول على عينة دم من خلال إحداث خنخز أو ثقب في
+
+758
+00:56:31,190 --> 00:56:34,850
+الوريد، يعني أني أحصل على عينة من الوريد، تمام؟
+
+759
+00:56:34,850 --> 00:56:39,770
+عينة blood، whole blood، طبعاً مجرد ما حصلت على العينة
+
+760
+00:56:39,770 --> 00:56:43,630
+هذه، بفضيها في الأنبوبة المناسبة، سواء كانت أنبوبة
+
+761
+00:56:43,630 --> 00:56:46,950
+plain أو فيها EDTA أو فيها citrate أو heparin، حسب
+
+762
+00:56:46,950 --> 00:56:51,790
+نوع الفحص، طبعاً كل فحص بده بيتطلب نوع معين من الـ
+
+763
+00:56:51,790 --> 00:56:57,390
+tube، طبعاً، وطبعاً بعمل mix وبعمل labeling منيح، و
+
+764
+00:56:57,390 --> 00:57:01,720
+بارسلها للمختبر لحتى نجري الفحص عليها، هيك بنكون
+
+765
+00:57:01,720 --> 00:57:05,720
+خلصنا الجزئية اليوم، محاضرتنا الـ part one والـ part
+
+766
+00:57:05,720 --> 00:57:09,700
+two لـ blood collection، واتعرفنا على أني
+
+767
+00:57:09,700 --> 00:57:12,440
+كيف أسحب عينة إما من الـ skin سواء كان finger أو
+
+768
+00:57:12,440 --> 00:57:16,800
+heel أو من الوريد في الـ vein puncture، وتعلمنا
+
+769
+00:57:16,800 --> 00:57:22,600
+الأشياء النظرية المتعلقة بسحب العينة، تمام؟
+
+770
+00:57:22,600 --> 00:57:27,830
+المتعلقة بسحب العينة، بيضل الأشياء العملية، أول شيء
+
+771
+00:57:27,830 --> 00:57:30,790
+راح أشرح لكم .. راح أورجي لكم فيديو على الـ YouTube
+
+772
+00:57:30,790 --> 00:57:35,650
+تمام؟ وأشرح لكم الخطوات، يعني أرجع أذكركم
+
+773
+00:57:35,650 --> 00:57:41,650
+بالتعليمات اللي حكيناها، وأيضاً راح أصور لكم فيديو
+
+774
+00:57:41,650 --> 00:57:46,030
+اللي أنا .. أنا عم بسحب عينة الدم، وأورجي لكم الأدوات
+
+775
+00:57:46,030 --> 00:57:50,970
+اللي أنا بستخدمها ومكوناتها، بحيث أن نستذكر ونـ
+
+776
+00:57:50,970 --> 00:57:55,320
+.. ن .. نثبت المعلومات اللي ذكرناها خلال المحاضرة
+
+777
+00:57:55,320 --> 00:57:57,820
+يعطيكم العافية، موثقين بإذن الله
diff --git a/PL9fwy3NUQKwY9jl8xw-bWkVZkWcGGyspd/C6AH0Ptcsrk_raw.srt b/PL9fwy3NUQKwY9jl8xw-bWkVZkWcGGyspd/C6AH0Ptcsrk_raw.srt
new file mode 100644
index 0000000000000000000000000000000000000000..05517c017ffc4858c67af93da2ffcd5b183ee484
--- /dev/null
+++ b/PL9fwy3NUQKwY9jl8xw-bWkVZkWcGGyspd/C6AH0Ptcsrk_raw.srt
@@ -0,0 +1,3116 @@
+1
+00:00:05,480 --> 00:00:09,460
+السلام عليكم ورحمة الله وبركاته نستكمل مع بعض ان
+
+2
+00:00:09,460 --> 00:00:13,080
+شاء الله الجزء الثاني من المحاضرة بعنوان ال van
+
+3
+00:00:13,080 --> 00:00:17,080
+puncture أي اللي هو الحصول على عينة من الوريد ال
+
+4
+00:00:17,080 --> 00:00:20,560
+van puncture must be performed with care يعني لازم
+
+5
+00:00:20,560 --> 00:00:27,020
+انا لما بدي اجري او بدي احصل علىعين الدم لازم أحصل
+
+6
+00:00:27,020 --> 00:00:30,560
+عليها بعناية إذا .. إذا بدي أحصل على عين الدم من
+
+7
+00:00:30,560 --> 00:00:35,440
+الوريد أحصل عليها بعناية ليش؟ لإنه the vein of the
+
+8
+00:00:35,440 --> 00:00:38,640
+patient are the main source of blood for testing
+
+9
+00:00:38,640 --> 00:00:42,740
+and the entry point for medication solutions and
+
+10
+00:00:42,740 --> 00:00:47,160
+the blood transfusion يعني لإن الوريد هو الوريد
+
+11
+00:00:47,160 --> 00:00:51,440
+للمريض هو ال .. ال .. المكان اللي بيسير من خلاله
+
+12
+00:00:51,440 --> 00:00:56,380
+contact مع المريضمن .. منه بيتم عملية ال testing
+
+13
+00:00:56,380 --> 00:01:01,240
+يعني احنا بنحصل على عينة و .. يعني بيصير إجراء
+
+14
+00:01:01,240 --> 00:01:04,980
+افحوصات عليها أيضا هو ال entry point لل medication
+
+15
+00:01:04,980 --> 00:01:11,940
+من خلاله بيتم إدخال أدوية أو محاليل للمريض و أيضا
+
+16
+00:01:11,940 --> 00:01:17,020
+من خلال ال .. الوريد أيضا يتم ..نقل دم إذا استدعى
+
+17
+00:01:17,020 --> 00:01:22,360
+القمر فبالتالي إذا لازم أنا أتعامل مع الوريد بحذر
+
+18
+00:01:22,360 --> 00:01:26,020
+علشان أنه أنا ما أقعد في المريض because there are
+
+19
+00:01:26,020 --> 00:01:29,760
+only limited number of easily accessible vein لإنه
+
+20
+00:01:29,760 --> 00:01:34,200
+أنا في عندى عدد محدود من الأوردة اللى سهل الوصول
+
+21
+00:01:34,200 --> 00:01:37,400
+إليها في المريض طبعا it is important that
+
+22
+00:01:37,400 --> 00:01:40,860
+everything to be done to preserve their good
+
+23
+00:01:40,860 --> 00:01:44,080
+condition and availabilityطبعا عشان أنا في عندي
+
+24
+00:01:44,080 --> 00:01:47,860
+عدد محدود من الأوردة لازم أضمن أنه كل إشي عم
+
+25
+00:01:47,860 --> 00:01:53,500
+بيشتغل أنا بحضره أو بشتغله في .. أنه يكون هو يعني
+
+26
+00:01:53,500 --> 00:01:58,760
+بأحسن ظروفه و ب .. ضامن ال quality تبعته و ال .. و
+
+27
+00:01:58,760 --> 00:02:02,160
+ال availability تبعته يعني ال good practice طبعا
+
+28
+00:02:02,160 --> 00:02:05,520
+يعني ال good practice يعني أن أنا لما أسحب عين
+
+29
+00:02:05,520 --> 00:02:10,620
+الدم أسحبها ب .. ب .. اتباع التعليمات بحذافيرها
+
+30
+00:02:10,620 --> 00:02:14,580
+بحيث أني أناما اضطر ان انا ابحث على وريد اخر العدد
+
+31
+00:02:14,580 --> 00:02:19,280
+محدود تمام part of this responsibility lies on the
+
+32
+00:02:19,280 --> 00:02:22,700
+medical technologist طبعا الجزء من هذه العملية
+
+33
+00:02:22,700 --> 00:02:27,840
+بيقع على كاهل خصائي العلم الطبية المخبري او حتى
+
+34
+00:02:27,840 --> 00:02:31,900
+كمان الجزء الكبير اذا ما كانت المسئولية كلهاthe
+
+35
+00:02:31,900 --> 00:02:35,540
+ideal procedure is to have the patient lie down
+
+36
+00:02:35,540 --> 00:02:40,020
+افضل طريقة في الحصول على عين الدم من الوريد ان انا
+
+37
+00:02:40,020 --> 00:02:45,960
+اخلي المريض يستلقي if this is not possible طبعا
+
+38
+00:02:45,960 --> 00:02:54,720
+اذا مش متاح سرير او يعني كرسي يسمحله ان هويتمدد
+
+39
+00:02:54,720 --> 00:02:57,920
+the patient should sit in a sturdy comfortable
+
+40
+00:02:57,920 --> 00:03:02,280
+chair with his or her arm firmly supported on the
+
+41
+00:03:02,280 --> 00:03:07,180
+table or chair arm and is easily accessible to the
+
+42
+00:03:07,180 --> 00:03:11,000
+technologist هلأ إذا ما كان متاح أن يكون في عندي
+
+43
+00:03:11,000 --> 00:03:15,820
+سرير أو يكون في شخص يعنيكرسي أستلقي عليه المريض
+
+44
+00:03:15,820 --> 00:03:19,060
+طبعا في أحيانا كراسي بيرجع دهر تباعه ممكن أنه
+
+45
+00:03:19,060 --> 00:03:22,680
+يتمدد عليه يعني أنه ينفرقي في رد الكرسي فإنه يتمدد
+
+46
+00:03:22,680 --> 00:03:27,140
+عليه فساعتها إذا ما كان هذا الاشي متاح أنا بدي
+
+47
+00:03:27,140 --> 00:03:31,520
+أجلس المريض على كرسي مريح و ثابت و طبعا مثل ما
+
+48
+00:03:31,520 --> 00:03:36,110
+حكيناال .. في الجزء الأول إنه مايكونش الكرسي عالي
+
+49
+00:03:36,110 --> 00:03:41,250
+و يكون طبعا لازم يكون إله دهر و .. أساس إنه يمسك
+
+50
+00:03:41,250 --> 00:03:44,410
+المريض منيح في حال لو هم عليه أو فقد وعيه إن ما
+
+51
+00:03:44,410 --> 00:03:48,910
+ييجي على الأرض و تكون وجهته محرزة، حكيناتمام؟ طيب
+
+52
+00:03:48,910 --> 00:03:53,290
+a patient should never stand or .. or sit on a
+
+53
+00:03:53,290 --> 00:03:57,150
+high stool during any process of blood collection
+
+54
+00:03:57,150 --> 00:04:02,670
+مش لازم ال .. ال .. المريض يجلس على كرسي عالي
+
+55
+00:04:02,670 --> 00:04:07,090
+أثناء عملية السحب الدمع عشان احنا حكينا كل ما زودت
+
+56
+00:04:07,090 --> 00:04:11,130
+المسافة بينه و بين الأرض هتكون ال .. يعني الوجعة
+
+57
+00:04:11,130 --> 00:04:16,540
+خطرةتمام؟ طيب و طبعا لازم ال .. المريض بس يجلس على
+
+58
+00:04:16,540 --> 00:04:23,180
+الكرسي اللي هو مريح و آمن و بتوفر فيه كل الشروط
+
+59
+00:04:23,180 --> 00:04:28,880
+لازم انه ي .. يفرد ايده على ال .. ال bench او يا
+
+60
+00:04:28,880 --> 00:04:34,180
+طاولة او ال .. ايدي الكرسي بحيث انها تكون انه سهل
+
+61
+00:04:34,180 --> 00:04:37,640
+ال technology يصل لايده و يبحث عن الوريد المناسب
+
+62
+00:04:37,640 --> 00:04:42,070
+لحتى يسحب منه العينةإيش هي ال reagents و ال
+
+63
+00:04:42,070 --> 00:04:48,290
+equipment اللي لازم أنا أستخدمها? tourniquet تمام
+
+64
+00:04:48,290 --> 00:04:53,770
+و طبعا tourniquet إيش يعني؟ يعني ربطة علشان تعملي
+
+65
+00:04:53,770 --> 00:04:58,730
+ضغط على ال .. منطقة الوريد بحيث يبرز الوريد و يسهل
+
+66
+00:04:58,730 --> 00:05:03,620
+إيش أنه أنا أحصل على عينة من الوريدalcohol wipes
+
+67
+00:05:03,620 --> 00:05:07,520
+طبعا لازم يكون في عندي قطن مغموس بالالكهول 70%
+
+68
+00:05:07,520 --> 00:05:11,800
+ايزوبربانول او 70% ايثانول gauze sponge طبعا لازم
+
+69
+00:05:11,800 --> 00:05:18,940
+يكون في عندي قطن مثلا او شاش ادهيسف باندش حكاية
+
+70
+00:05:18,940 --> 00:05:26,020
+لازم يكون في عندي لاسق تمام بلاستر و لازم يكون في
+
+71
+00:05:26,020 --> 00:05:30,560
+عندي glovesإذا nitril أو latex أو vinyl حسب إيش
+
+72
+00:05:30,560 --> 00:05:35,140
+النوع المتاح أو متوفر طبعا استرال disposable
+
+73
+00:05:35,140 --> 00:05:39,760
+syringe هاي كلمة disposable اللي معناها أنه
+
+74
+00:05:43,030 --> 00:05:47,390
+هي كلمة disposable اللي معناها انه الاشي يستخدم
+
+75
+00:05:47,390 --> 00:05:51,630
+لمرة واحدة فقط، تمام؟ طيب، لازم يكون في عندى
+
+76
+00:05:51,630 --> 00:05:55,570
+سرنجات بستخدمها لمرة واحدة فقط، disposable syringe
+
+77
+00:05:55,570 --> 00:06:01,170
+و أيضا disposable needle و الإبرة، تمام؟ طيب، the
+
+78
+00:06:01,170 --> 00:06:04,330
+choice of needle depends on the size of the van
+
+79
+00:06:04,330 --> 00:06:10,660
+طبعا ال needle هي بنات بتكون أحجاماحنا لما بنختار
+
+80
+00:06:10,660 --> 00:06:15,980
+الإبرة بتكون أحجام أنا بختارها حسب الحجم الوريد،
+
+81
+00:06:15,980 --> 00:06:19,700
+إذا الوريد رفيع بختار الحجم ال .. ال needle أنه
+
+82
+00:06:19,700 --> 00:06:24,080
+يكون رفيع، تمام؟ تكون ال needle رفيع The most
+
+83
+00:06:24,080 --> 00:06:29,720
+commonly used needle طبعا ال needle يعبر عن قطرها
+
+84
+00:06:29,720 --> 00:06:33,940
+أو عن حجمها، السمك تبعها، رفعها أو هل هي رفيعة أو
+
+85
+00:06:33,940 --> 00:06:39,790
+سميكةبيعبر ب number اسمه gauge number، تمام؟ gauge
+
+86
+00:06:39,790 --> 00:06:47,170
+number و أيضا كمان طبعا بيعبر أيضا على .. عن
+
+87
+00:06:47,170 --> 00:06:52,710
+طولها، يعني كمان ال gauge number فهلأ ال .. زي ما
+
+88
+00:06:52,710 --> 00:06:56,490
+انتوا شايفين ال gauge number عشرين، واحد و عشرين،
+
+89
+00:06:56,490 --> 00:07:00,450
+اتنين و عشرين، تمام؟ هدولة ال most commonly used
+
+90
+00:07:01,540 --> 00:07:05,860
+طبعا هنا بحكيلك أنه the higher the gauge number
+
+91
+00:07:05,860 --> 00:07:11,480
+the smaller the inner diameter يعني كل ما زاد ال
+
+92
+00:07:11,480 --> 00:07:16,020
+gauge number كل ما قل ال diameter يعني كانت الإبرة
+
+93
+00:07:16,020 --> 00:07:20,170
+أرفع، تمام؟احنا لما بنيجي بناخد .. يعني ناخد إبرة
+
+94
+00:07:20,170 --> 00:07:23,630
+بالعضل بنحب ناخد ال .. في الإبرة الرفيعة عشان ما
+
+95
+00:07:23,630 --> 00:07:27,650
+نتوجع، تمام؟ يعني إيش الإبرة الرفيعة؟ يعني ال
+
+96
+00:07:27,650 --> 00:07:31,750
+gauge number العالي، يعني هدول التلات أرقام، مين
+
+97
+00:07:31,750 --> 00:07:35,550
+هم .. يعني أرفع إبرة فيهم؟ أرفع إبرة هي ال gauge
+
+98
+00:07:35,550 --> 00:07:39,610
+number اتنين وعشرين، وأسمك إبرة هي ال gauge عشرين،
+
+99
+00:07:39,610 --> 00:07:43,290
+تمام؟ إذا في علاقة عكسية ما بين الصمك، ما بين ال
+
+100
+00:07:43,290 --> 00:07:48,040
+diameter، القطر تبع الإبرةمابين ال gauge number
+
+101
+00:07:48,040 --> 00:07:51,500
+تمام؟ the length of the needle also chosen
+
+102
+00:07:51,500 --> 00:07:55,040
+according to the depth of the vein برضه كمان أنا
+
+103
+00:07:55,040 --> 00:08:00,440
+بختار طول الإبرة تبعا لعمق يعني إذا كانت العمق ال
+
+104
+00:08:00,440 --> 00:08:05,220
+.. ال .. الوريد عميق سميء لجوة تمام؟ بختار إبرة
+
+105
+00:08:05,220 --> 00:08:09,340
+طولة يعني طويلة عشان أنا أفوت لجوة أكتر تمام؟ أفوت
+
+106
+00:08:09,340 --> 00:08:14,950
+عمق يعني كبيرإذا كان الوريد سطحي فبختار قبرة طولها
+
+107
+00:08:14,950 --> 00:08:19,370
+قصير ولكن ال most commonly used depth واحد و نص
+
+108
+00:08:19,370 --> 00:08:22,730
+انش يعني انه يكون طول القبرة واحد و نص انش خلينا
+
+109
+00:08:22,730 --> 00:08:26,830
+بس انا اعمل skip بس لهذه ال slide و اشوف هذه
+
+110
+00:08:26,830 --> 00:08:31,070
+المكوناتالسرنج و ال needle هذه هي السرنجة طبعا
+
+111
+00:08:31,070 --> 00:08:35,550
+السرنجة زي ما انتوا شايفين مكونة من قطعتين قطعة
+
+112
+00:08:35,550 --> 00:08:39,950
+متحركة هذه القطعة المتحركة و هذه الثابتة طبعا
+
+113
+00:08:39,950 --> 00:08:44,930
+القطعة الثابتة بسميها barrel of syringe طبعا هذه
+
+114
+00:08:44,930 --> 00:08:49,310
+بتكون عليها تدريج ال barrel هذا هي عند 10 سانتي
+
+115
+00:08:49,310 --> 00:08:53,490
+مثلا عشرة أمقال فيه لخمسة أمقال فيه لتلاتة أمقال
+
+116
+00:08:53,490 --> 00:08:57,180
+فيه لاتنين أمقالإذا يعني الصرنجات مش كلها نفس
+
+117
+00:08:57,180 --> 00:09:06,200
+الحجم، تمام؟ وهذا الجزء المتحرك بسميه blangar زي
+
+118
+00:09:06,200 --> 00:09:09,080
+ال blangar تبع ال pipet هو الجزء المتحرك اللي أنا
+
+119
+00:09:09,080 --> 00:09:12,400
+كنت بضغط عليه، بكبس عليه كبسة أول أو كبسة تانية
+
+120
+00:09:12,400 --> 00:09:15,540
+علشان أخد الحجوم، صح؟ فهذا نفس الأشياء، ال blangar
+
+121
+00:09:15,540 --> 00:09:18,980
+هو الجزء المتحرك اللي بيرجع لورا عشان أنا أسحب
+
+122
+00:09:18,980 --> 00:09:26,220
+الحجم الدم المناسب من ال .. من الوريدطبعا حكينا
+
+123
+00:09:26,220 --> 00:09:30,240
+إنه لسرنجات هذه أحجام، طبعا أنا بختار حسب حجم
+
+124
+00:09:30,240 --> 00:09:33,100
+العينة اللي بدي إياها، إذا أنا بدي أعمل فحوصات
+
+125
+00:09:33,100 --> 00:09:37,140
+قليلة بختار حجم عينة قليل، يعني السرنجة قليل، يعني
+
+126
+00:09:37,140 --> 00:09:40,920
+بنهاية كمان بفضيها في تيوب صغيرة، حكينا عن حجوم
+
+127
+00:09:40,920 --> 00:09:46,610
+للتيوبات المحاضرة الناضيةطيب إذا هذا ما يتعلق بال
+
+128
+00:09:46,610 --> 00:09:51,370
+syringe disposable باستخدام واحد فقط يعني برميها
+
+129
+00:09:51,370 --> 00:09:55,330
+بعد ما أنا أسحبتيها ما بستخدمها لمريض آخر و هذه ال
+
+130
+00:09:55,330 --> 00:09:59,310
+needle أيضا disposable الإبرة هاي هي ال needle زي
+
+131
+00:09:59,310 --> 00:10:03,290
+ما أنتوا شايفين الإبرة أو ال needle مكونة من اللي
+
+132
+00:10:03,290 --> 00:10:07,890
+هو الجزء المعدني و جزء بلاستيكي مكون ملون أزرق
+
+133
+00:10:07,890 --> 00:10:12,250
+أخضر رمادي حسب نوع لون الإبرة تمام؟ طب هذا ال ..
+
+134
+00:10:12,250 --> 00:10:17,440
+ال ..الجزء بيكون مكتوب عليه ال gauge number طبعا
+
+135
+00:10:17,440 --> 00:10:22,340
+هي العشرين هي شايفين أسمك واحدة تمام؟ و أطول واحدة
+
+136
+00:10:23,310 --> 00:10:29,170
+عشرين أقل سمكا، واحد وعشرين أقل سمكا، أرفع واحدة
+
+137
+00:10:29,170 --> 00:10:33,570
+اللي هي الخمسة وعشرين، إذا كل ما زاد ال .. ال ..
+
+138
+00:10:33,570 --> 00:10:37,850
+ال gauge number، تمام؟ هي عشرين، هي زي ما هذه
+
+139
+00:10:37,850 --> 00:10:42,150
+عشرين نفسها، تمام؟ إذا نفس السمك ونفس الطول، ال
+
+140
+00:10:42,150 --> 00:10:47,210
+gauge واحد وعشرين أقل سمكا، الخمسة وعشرين أقل
+
+141
+00:10:47,210 --> 00:10:51,790
+سمكا، أرفع واحدة، تمام؟ وهي أكثرفبالتالي إذا كل ما
+
+142
+00:10:51,790 --> 00:10:55,710
+زاد ال gauge number كل ما قل الصمت، تمام؟ طيب
+
+143
+00:10:55,710 --> 00:10:59,930
+الجزء المعدني هذا زي ما أنتوا شايفين مكون من ال
+
+144
+00:10:59,930 --> 00:11:03,870
+shaft اللي هو يعني الجزء الإبرة، الاستوانة طبعة
+
+145
+00:11:03,870 --> 00:11:10,990
+الإبرة و جزء منها مدبّب و في فتحة بسميها ال bevel،
+
+146
+00:11:10,990 --> 00:11:13,970
+الفتحة هذه بسميها ال bevel of needle، فتحة ال
+
+147
+00:11:13,970 --> 00:11:17,960
+needle، تمام؟ال bevel of needle مهم جدا هذه
+
+148
+00:11:17,960 --> 00:11:22,760
+الجزيئات نعرف تركيبة ال needle و مكوناتها،
+
+149
+00:11:22,760 --> 00:11:26,700
+جزيئاتها و نعرف تركيبة السرنج عشان كمان شوية لما
+
+150
+00:11:26,700 --> 00:11:30,740
+نشرح باق المحاضرة أو الجزء المتبقي من المحاضرة أنه
+
+151
+00:11:30,740 --> 00:11:34,220
+هصير نحكي عن ال bevel، تمام؟ أذكر ال bevel كتير
+
+152
+00:11:34,220 --> 00:11:38,310
+لنعرف أن ال bevel هو فتحة ال needle، تمام؟الجزء
+
+153
+00:11:38,310 --> 00:11:42,470
+اللي .. اللي هو ال .. زي مشطوف، بيكون شفتوا ال ..
+
+154
+00:11:42,470 --> 00:11:45,950
+شكل المكارونة بالبشاميل كانت بيكون جاية بزاوية،
+
+155
+00:11:45,950 --> 00:11:50,810
+نفس الاشي فاتحت ال .. ال .. ال needle بتكون جاية
+
+156
+00:11:50,810 --> 00:11:56,870
+بزاويةتمام نرجع على ال slide اللي تركناها ال evac
+
+157
+00:11:56,870 --> 00:12:00,030
+.. ال vacotainer system ال vacotainer system هو
+
+158
+00:12:00,030 --> 00:12:06,050
+انه بيكون عندى ال syringe هذه كبيرة و طبعا ويلها
+
+159
+00:12:06,050 --> 00:12:10,590
+needle مشبوك فيها needle تمام؟ و هاد ال .. ال
+
+160
+00:12:10,590 --> 00:12:14,570
+syringe طبعا أنا بفوتها في وريد المريض و بستخدم
+
+161
+00:12:14,570 --> 00:12:19,610
+أنبيب خاصة بفوتهم داخل ال .. ال .. داخل ال syringe
+
+162
+00:12:19,610 --> 00:12:24,300
+هذه طبعا و بيصير يعبي فيها مباشرةإنه .. إنه هذه
+
+163
+00:12:24,300 --> 00:12:31,100
+السرنجة بتكون متصلة بإبرة، الإبرة هذه بتاخد ال ..
+
+164
+00:12:31,100 --> 00:12:33,900
+يعني جزء منها بيكون في الوريد و جزء منها بيكون
+
+165
+00:12:33,900 --> 00:12:37,960
+بيخترق ال tube هورجيكم إياها إن شاء الله من خلال
+
+166
+00:12:37,960 --> 00:12:44,140
+video اللي بيشرح عملية السحب فهتشوفوا بإذن الله
+
+167
+00:12:44,140 --> 00:12:48,700
+كيف احنا يعني كيف بنستخدم .. بنجمع عين الدم
+
+168
+00:12:48,700 --> 00:12:51,420
+باستخدام needle و سرنج أو باستخدام ال vacotainer
+
+169
+00:12:51,420 --> 00:12:56,040
+systemتمام؟ طيب ال vacotainer system هو عبارة عن
+
+170
+00:12:56,040 --> 00:13:01,040
+أنه أنا بستخدم أنابيب evacuated يعني مفرغة من
+
+171
+00:13:01,040 --> 00:13:05,280
+الهواء و هاد ال .. ال .. ال .. ال أنابيب designed
+
+172
+00:13:05,280 --> 00:13:09,160
+to fill with a predetermined volume by .. of a
+
+173
+00:13:09,160 --> 00:13:12,800
+blood by vacuumيعني من .. هي مفرغة من الهواء أو
+
+174
+00:13:12,800 --> 00:13:17,420
+على جد ما هي مفرغة من الهواء بتاخد حجم مناسب للدم،
+
+175
+00:13:17,420 --> 00:13:20,680
+يعني لما أنا ب .. بعبي فيها الدم بتاخد حجم مناسب
+
+176
+00:13:20,680 --> 00:13:24,940
+على حسب هي .. على قدر ما هي مفرغة من الهواء، تمام؟
+
+177
+00:13:25,970 --> 00:13:29,270
+طبعا غطيتها بتكون مصنوعة من ال rubber هذه القنابيب
+
+178
+00:13:29,270 --> 00:13:32,770
+بحيث أنه يسهل ثقبها من خلال ال needle اللي بتكون
+
+179
+00:13:32,770 --> 00:13:36,070
+موجودة في ال syringe اللي أنا حكيتلكم عنها طبعا في
+
+180
+00:13:36,070 --> 00:13:43,010
+عندي عدة .. انا عدة أحجوم متوفرة حسب .. قولتلكم أن
+
+181
+00:13:43,010 --> 00:13:46,550
+القنابيب حجامها مختلفة فبالتالي حسب حجم العينة أنا
+
+182
+00:13:46,550 --> 00:13:50,300
+باخد النبوبة المناسبةBlood should never be poured
+
+183
+00:13:50,300 --> 00:13:53,640
+from one tube to another since that tube can have
+
+184
+00:13:53,640 --> 00:13:58,700
+different additive or coating طبعا ال .. ال .. ال
+
+185
+00:13:58,700 --> 00:14:03,000
+tubeات لإن محتوها يعني أنا بستخدمها لما بدي أسحب
+
+186
+00:14:03,000 --> 00:14:06,640
+أكتر من عينة في أكتر من انبوبة و الانبوبة دي فيها
+
+187
+00:14:06,640 --> 00:14:10,420
+أكتر من محتوى يعني مثلا بدي أسحب عينة سترة و عينة
+
+188
+00:14:10,420 --> 00:14:14,670
+هبارين و عينة إيدة و عينة بلانطبعا هدول يعني انا
+
+189
+00:14:14,670 --> 00:14:19,510
+.. يعني بدي كمية يعني ف .. يعني بحضر القنابيب و
+
+190
+00:14:19,510 --> 00:14:22,750
+على طول ب .. بفو .. بفوتهم داخل ال .. ال needle
+
+191
+00:14:22,750 --> 00:14:27,050
+اللي .. اللي انا حكيتلكم عنها و بيصير يعبي داخلها
+
+192
+00:14:27,050 --> 00:14:32,980
+من الوريد الى ال tube مباشرة، تمام؟طبعا مكون ايضا
+
+193
+00:14:32,980 --> 00:14:35,480
+اذا هذا ال vacuum cleaner system مكون من ليتيوبات
+
+194
+00:14:35,480 --> 00:14:39,120
+المفرغة من الهواء مكون من ال needle ال needle هذه
+
+195
+00:14:39,120 --> 00:14:42,500
+طبعا انه the gauge number indicate the pore size
+
+196
+00:14:42,500 --> 00:14:47,260
+قولنا كل ما جاء زاد ال gauge number هيقل ال .. ال
+
+197
+00:14:47,260 --> 00:14:51,520
+needle pore و طبعا هذه ال needle يا بنات زي ما
+
+198
+00:14:51,520 --> 00:14:58,240
+حكيتلكم جزءين جزء موجود خارج ال syringe و ب ..
+
+199
+00:15:01,570 --> 00:15:04,990
+بيخترق الوريد وجزء آخر موجود داخل الـ syringe
+
+200
+00:15:04,990 --> 00:15:09,090
+بيخترق فتحة ال tube تمام؟ عشان هيك ال YouTube
+
+201
+00:15:09,090 --> 00:15:15,630
+غطيتها من المطاط طبعا في holder أو adapter موجود
+
+202
+00:15:15,630 --> 00:15:17,970
+في هذا ال vacuum container system على أساس إن أنا
+
+203
+00:15:17,970 --> 00:15:21,550
+من خلاله أمسك ال tube و أثبتها أثناء السحب وفي
+
+204
+00:15:21,550 --> 00:15:25,890
+عنده اللي هي ال needle disposable unitإنه needle
+
+205
+00:15:25,890 --> 00:15:31,670
+should never .. طبعا إنه يعني ال .. بعد ما أنا
+
+206
+00:15:31,670 --> 00:15:35,410
+أخلصها ممكن أنا يبقى أفك ال needle و أتخلص منها و
+
+207
+00:15:35,410 --> 00:15:38,250
+أركب needle جديدة يعني هذه ال needle ممكن تتغير
+
+208
+00:15:38,250 --> 00:15:42,770
+طبعا برجع بكرر القاعدة اللي أنا ذكرت و ركزت عليها
+
+209
+00:15:42,770 --> 00:15:45,550
+كتير المحاضرة الماضية أو الجزء الأول من المحاضرة
+
+210
+00:15:45,550 --> 00:15:51,150
+إن هو neverbroke, bent, recap needle ممنوع أكسر أو
+
+211
+00:15:51,150 --> 00:15:56,370
+أتني أو أرجع أغطي ال needle المستعملة تمام عشان
+
+212
+00:15:56,370 --> 00:15:59,290
+أتجنب إن أنا أقضي حالي أجرح نفسي أو أنخذ نفسي في
+
+213
+00:15:59,290 --> 00:16:02,770
+ال needle المستعملة needles should be placed in a
+
+214
+00:16:02,770 --> 00:16:06,590
+proper disposable unit لازم أتخلص منها immediately
+
+215
+00:16:06,590 --> 00:16:11,110
+فورا في ال sharp box في ال disposable unit تبعتها
+
+216
+00:16:11,110 --> 00:16:13,530
+يعني في ال container تبعها الخاص فيها اللي هو
+
+217
+00:16:13,530 --> 00:16:18,590
+sharp box تمام؟طيب هذا بالنسبة ل ال .. ال .. ال
+
+218
+00:16:18,590 --> 00:16:21,890
+vacotainer system طبعا ال procedure طبعتي كيف أنا
+
+219
+00:16:21,890 --> 00:16:26,370
+بدي أسحب عينة من الوريد أول إشي ب .. بختار الوريد
+
+220
+00:16:26,370 --> 00:16:29,690
+إما اللي هو ال median cubital أو ال cephalic vein
+
+221
+00:16:29,690 --> 00:16:36,430
+طبعا في منطقة ال .. الكوة ال .. ال .. في منطقة
+
+222
+00:16:36,430 --> 00:16:42,210
+الكوة طبعاإذن بختار ال .. ال .. إما ال median
+
+223
+00:16:42,210 --> 00:16:45,690
+cubital أو ال cephalic vein of the arm are used
+
+224
+00:16:45,690 --> 00:16:50,610
+more frequently و طبعا إذا كان ال .. ال .. ال rest
+
+225
+00:16:50,610 --> 00:16:54,250
+و ال hand vein are also acceptable أحيانا بناخد
+
+226
+00:16:54,590 --> 00:17:01,150
+يعني ال .. الأورده اللي موجودين في قبضة الإيد بس
+
+227
+00:17:01,150 --> 00:17:04,570
+يعني بيفضل أن أنا أبعد عنهم و أتجنبهم لإن هم
+
+228
+00:17:04,570 --> 00:17:08,130
+مؤلمين أحيانا بكونوا فالأفضل أن أنا أستخدم في
+
+229
+00:17:08,130 --> 00:17:11,710
+منطقة اللي هي الكوع في ال .. في المنطقة اللي هي
+
+230
+00:17:11,710 --> 00:17:14,970
+اللي بنسميها Anticubital Area في المنطقة اللي
+
+231
+00:17:14,970 --> 00:17:21,230
+اسمها Anticubital Areaand trace the path of the
+
+232
+00:17:21,230 --> 00:17:25,990
+vein with the index finger بأصبعك السبابة تحسسي
+
+233
+00:17:25,990 --> 00:17:30,370
+الوريد و حاولي أنك تشوفي مصاره و تعرفي اتجاهه ليش؟
+
+234
+00:17:30,370 --> 00:17:34,370
+علشان انا من خلال .. انا بدي افوت .. اكون محادية
+
+235
+00:17:34,370 --> 00:17:40,230
+لاتجاهه علشان افوت صح داخل الوريد تمام؟الوريد كيف
+
+236
+00:17:40,230 --> 00:17:44,290
+أنا بحسه؟ بحس أنه في إشي مطاطي تحت إيدي تحت إصبع
+
+237
+00:17:44,290 --> 00:17:48,130
+السبابة يعني أنا لما بضغط عليه بحسه بطلع و بينزل
+
+238
+00:17:48,130 --> 00:17:54,470
+زي كإني بضغط على انبوب مطاطي أو على إشي زي مثلا
+
+239
+00:17:54,470 --> 00:18:00,330
+مغيطة تمام؟ و طبعا إله .. الوريد إله thick wall
+
+240
+00:18:00,330 --> 00:18:08,660
+إذا كان الوريد superficial إذا كان سطحي ف ..طبعا
+
+241
+00:18:08,660 --> 00:18:13,720
+يعني لازم انا افوتله ب .. اذا بكمية .. يعني افوت
+
+242
+00:18:13,720 --> 00:18:19,740
+سطحية، اذا كان طبعا الوريد عميق، بدي افوت بعمق،
+
+243
+00:18:19,740 --> 00:18:23,380
+تمام؟ if superficial veins are not readily
+
+244
+00:18:23,380 --> 00:18:27,080
+apparent طب لو ماكنش مبين ال superficial veins،
+
+245
+00:18:27,080 --> 00:18:31,120
+إيش بدني أعمل؟ you can force blood into the vein
+
+246
+00:18:31,120 --> 00:18:36,490
+by massaging the armfrom wrist to elbow, tap the
+
+247
+00:18:36,490 --> 00:18:41,410
+site with index and second finger, apply warm damp
+
+248
+00:18:41,410 --> 00:18:47,990
+to the site for five minutes يعني لو أنا ماشوفتش
+
+249
+00:18:47,990 --> 00:18:52,390
+الوريد معناها إن أنا بدي أضغط على المنطقة أو بدي
+
+250
+00:18:52,390 --> 00:18:56,370
+أعمل massaging يمتد من عند اللي هي ال wrist اللي
+
+251
+00:18:56,370 --> 00:19:05,090
+هومن عند الرسغ لحد القلب و الكوع و بقى او ممكن انا
+
+252
+00:19:05,090 --> 00:19:08,530
+يعني اض .. اتحسس من خلال ان انا اشوف الوريد
+
+253
+00:19:08,530 --> 00:19:12,510
+بالسبابة ممكن انا ادف .. ادف المكان من خلال ان انا
+
+254
+00:19:12,510 --> 00:19:17,550
+احط قطعة قماش لمدة خمس دقائق تكون دافئة او اني
+
+255
+00:19:17,550 --> 00:19:21,970
+انزل الإيد لأدنى مستوى الجسم حكينا انه ساعتها
+
+256
+00:19:21,970 --> 00:19:26,050
+بيتضفق الدم بفعل الجاذبية لأسفل الإيد و بتبين
+
+257
+00:19:26,050 --> 00:19:30,010
+بتبرز الأوردةزي ما حكينا أو شرحنا بالمحاضرة أو
+
+258
+00:19:30,010 --> 00:19:33,450
+بالجزء الأول من المحاضرة، هي منطقة الـ Anticubital
+
+259
+00:19:33,450 --> 00:19:36,630
+يا بنات، هي منطقة الكوع، أنا من هنا بسحب عينة
+
+260
+00:19:36,630 --> 00:19:40,410
+الدم، وهنا الرسغ، فأنا بدأ أعمل massage لو الوريد،
+
+261
+00:19:40,410 --> 00:19:43,670
+بعمل massage بهذا الشكل، بحيث أنه أنا أحرك الدم
+
+262
+00:19:43,670 --> 00:19:47,510
+بهذا الاتجاه عشان يبرز الوريد عندي هنا أو أحط قطعة
+
+263
+00:19:47,510 --> 00:19:51,700
+قماش دافعةطبعا إيش الأوردة المناسبة؟ هاي زي ما
+
+264
+00:19:51,700 --> 00:19:54,700
+انتوا شايفين دا قطوعها عامل زي .. اللي هي رقم 7
+
+265
+00:19:54,700 --> 00:19:59,220
+اللي هم ال main .. ال median cubital تمام؟ ال
+
+266
+00:19:59,220 --> 00:20:02,200
+median cubital van و ال cephalic van هدول أفضل
+
+267
+00:20:02,200 --> 00:20:05,500
+أوردة تمام؟ ممكن أنا أروح على الأوردة اللي هنا
+
+268
+00:20:05,500 --> 00:20:07,960
+اللي على .. اللي ال deep أو اللي على الأطراف هنا
+
+269
+00:20:07,960 --> 00:20:13,980
+ولكن هذا يعني ..بي .. بيختار .. بيعتمد حسب حالة
+
+270
+00:20:13,980 --> 00:20:17,660
+المريض حسب إيش الوريد الواضح عنده أحيانا بتكون
+
+271
+00:20:17,660 --> 00:20:20,320
+اللي على الأطراف هنا مؤلمة فالأفضل أن أنا أكون في
+
+272
+00:20:20,320 --> 00:20:23,500
+منطقة المنتصف قدر المستطاع يعني في ال cubital و
+
+273
+00:20:23,500 --> 00:20:28,000
+الcephalic تمام؟ هذا هو مع الممارسة يا بنات هتسيري
+
+274
+00:20:28,000 --> 00:20:32,880
+تعرفي ال anatomy تبع الأيد تعرفي مكان الأوردة منك
+
+275
+00:20:32,880 --> 00:20:36,300
+لحالك يعني مجرد ما ت .. يعني فوت ال needle بتعتري
+
+276
+00:20:36,300 --> 00:20:41,530
+على الوريد و بتحصلي على الدم تمام؟يعني الاشي بده
+
+277
+00:20:41,530 --> 00:20:45,590
+ممارسة ان شاء الله هنرجع المختبرات هنعلمكم هو هسحب
+
+278
+00:20:45,590 --> 00:20:50,470
+قدامكم هخليكم تسحبوا من بعض و تجيبوا يعني ناس مثلا
+
+279
+00:20:50,470 --> 00:20:53,790
+متبرعين volunteer تسحبوا منهم هعطيكم أدوات تسحبوا
+
+280
+00:20:53,790 --> 00:20:57,910
+من أهلكم في البيت فيعني هعلمكم ان شاء الله أسحب و
+
+281
+00:20:57,910 --> 00:21:03,190
+لما أتأكدنكم يعني أتقنتوا هخليكم يعني أنه تجربوا
+
+282
+00:21:03,190 --> 00:21:07,870
+على إما متطوعين أو حدا من أهلكملحتى انه تتقنوا
+
+283
+00:21:07,870 --> 00:21:11,290
+الاشي اكثر و اكثر و تمارسوا اكثر و اكتر تمام
+
+284
+00:21:11,290 --> 00:21:15,270
+performance of vent puncture انا هلأ يعني اتعرفت
+
+285
+00:21:15,270 --> 00:21:18,450
+على المنطقة اللي بدي اسحب منها و عرفت ايش الأدوات
+
+286
+00:21:18,450 --> 00:21:23,380
+اللي بدي احضرهم بدي اجري ال vent punctureأول إشي
+
+287
+00:21:23,380 --> 00:21:28,540
+طبعا ال .. ال slide زي ما انتوا شايفين مكررة وردت
+
+288
+00:21:28,540 --> 00:21:31,740
+معانا في ال skin puncture بدي أتعامل مع المريض
+
+289
+00:21:31,740 --> 00:21:36,260
+بشكل friendly بشكل calm manner بهدوء ما أكون
+
+290
+00:21:36,260 --> 00:21:41,600
+aggressive معه أحاول إن أنا أكون ودودة لطيفة معاه
+
+291
+00:21:41,600 --> 00:21:46,220
+طبعا أريحه أحاول إن أنا أريحه إذا كان هو متوتر
+
+292
+00:21:46,220 --> 00:21:51,370
+أخليه ياخد نفس عميقيستهل .. يستلقي .. يسترخي مثلا
+
+293
+00:21:51,370 --> 00:21:55,330
+إذا هو خايف من ال .. ال procedure أحكيله مثلا يلف
+
+294
+00:21:55,330 --> 00:21:57,870
+وجهه على الجهة التانية عشان مايشوف أنا إيش بعمل
+
+295
+00:21:57,870 --> 00:22:03,070
+طبعا بدي أخليه يتعاون معايا بدي أتعرف عليه منيح
+
+296
+00:22:03,070 --> 00:22:05,850
+identify the patient correctly و يعرفني إيش يعني
+
+297
+00:22:05,850 --> 00:22:09,130
+كيف أنا أتعرف على المريض منيح و أتأكد إن هو هذا
+
+298
+00:22:09,130 --> 00:22:12,150
+المريض الصح مع ال request الصح عشان أسحب العين
+
+299
+00:22:12,150 --> 00:22:17,430
+الصحو بدي أتعابى الجزء اللى خاص فيا بال request
+
+300
+00:22:17,430 --> 00:22:20,950
+form اللى هو يعني ال date و ال time و ال initial
+
+301
+00:22:20,950 --> 00:22:26,210
+تبعى أيضا بدي أتأكد هل المريض مستوفى لشروط إجراء
+
+302
+00:22:26,210 --> 00:22:31,910
+الفحص صايم مش صايمماكل إشي معين، متجنب أكل معين،
+
+303
+00:22:31,910 --> 00:22:36,090
+مااخد دواء، في فحوصات حكينالكم تجرى صباحا، في
+
+304
+00:22:36,090 --> 00:22:40,110
+فحوصات تجرى مساقا، في فحوصات تجرى بعد exercise،
+
+305
+00:22:40,110 --> 00:22:43,830
+بعد ما ياخد تمرين رياضة، بعد ما ياخد جرعة سكر أو
+
+306
+00:22:43,830 --> 00:22:47,390
+.. أو إشي معين، فبالتالي أنا لازم أتأكد إن هو
+
+307
+00:22:47,390 --> 00:22:51,330
+مستوى في الشروط لإجراء الفحص. بدي أسأله صايم؟ طب
+
+308
+00:22:51,330 --> 00:22:56,490
+كام ساعة صومت؟ عشان أتأكد من متى لمتى؟صمت طب أكلت
+
+309
+00:22:56,490 --> 00:22:59,150
+إشي؟ طب إيش الأدوية اللي تاخدها؟ كل هذه الأشياء
+
+310
+00:22:59,150 --> 00:23:03,570
+لازم أنا أتأكد منها تبعا لنوع الفحص اللي مطلوب
+
+311
+00:23:03,570 --> 00:23:07,550
+prepare the blood collection assembly طبعا لازم
+
+312
+00:23:07,550 --> 00:23:10,910
+أحضر الأدوات تبعتي يعني أركب ال syringe و ال
+
+313
+00:23:10,910 --> 00:23:14,570
+needle و أتأكد إن ال syringe .. ال syringe يا بنات
+
+314
+00:23:14,570 --> 00:23:19,630
+ال plunger تبعها بيكون hectares فلازم ألينه و أخلي
+
+315
+00:23:19,630 --> 00:23:24,000
+أتأكد من إنه بيروح و بيجي بسهولةجابل أجي أصحى
+
+316
+00:23:24,000 --> 00:23:31,460
+بالعينة، تمام؟ و طبعا بتأكد أنه ب .. ب .. بحرك ال
+
+317
+00:23:31,460 --> 00:23:34,080
+needle .. بركب ال needle و بحركها بحيث أن ال bevel
+
+318
+00:23:34,080 --> 00:23:40,340
+تبعتها، الفتحة تبعتها تكون مقابلة لإيش؟ لالتدريج،
+
+319
+00:23:40,340 --> 00:23:43,400
+تكون الفتحة مقابلة للتدريج اللي موجود على ال
+
+320
+00:23:43,400 --> 00:23:49,780
+syringe، تمام؟طيب فإذا إذا بركب الأدوات تبعتي ال
+
+321
+00:23:49,780 --> 00:23:51,940
+needle is firmly adjusted to the tip of the
+
+322
+00:23:51,940 --> 00:23:55,140
+syringe بركب ال needle كويس على ال syringe it
+
+323
+00:23:55,140 --> 00:23:58,360
+check is then made to see that the plunger is not
+
+324
+00:23:58,360 --> 00:24:02,000
+stick with the barrel بتأكد أنه ال plunger مش
+
+325
+00:24:02,000 --> 00:24:07,700
+ملتصق بال barrel يعني مش مش تارس طبعا لأ بالعكسلين
+
+326
+00:24:07,700 --> 00:24:14,390
+بخليه بحركه مرة مرتين تلاتة بحيث أنهأتأكد إن هو
+
+327
+00:24:14,390 --> 00:24:19,470
+صار ليّن بعدين طبعا بجلس ال patient position ده
+
+328
+00:24:19,470 --> 00:24:26,450
+patient بجلسه على كرسي أو بخليه يستلقي و بيمدلي
+
+329
+00:24:26,450 --> 00:24:31,570
+إيده تمام و بعد هيك إيش بده أعمل apply the tear
+
+330
+00:24:31,570 --> 00:24:34,810
+naked من تلاتة لاربع inches above the selected
+
+331
+00:24:34,810 --> 00:24:39,630
+puncture site مكان ما أنا بدي أسحب العينة بروح بحط
+
+332
+00:24:39,630 --> 00:24:44,940
+ال tear naked لفوقها تمام؟يعني باتجاه الكتف لفوق
+
+333
+00:24:44,940 --> 00:24:49,640
+من تلاتة لاربع inches طبعا طب كيف هذي بدي اخدها
+
+334
+00:24:49,640 --> 00:24:54,920
+يعني انا بحط يعني فوق منطقة الكوع بتلت لاربع أصابع
+
+335
+00:24:54,920 --> 00:24:59,400
+بحط تلت أصابع من إيدي و بحدد المنطقة اللي انا بده
+
+336
+00:24:59,400 --> 00:25:01,400
+اربط فيها ال turnicut زي ما انتوا شايفين ال
+
+337
+00:25:01,400 --> 00:25:05,300
+turnicut هي عبارة عن ربطة مطاطية او حزام بربط فيه
+
+338
+00:25:05,300 --> 00:25:10,080
+بحيث انه يعملي ضغط على منطقة الكوع بحيث تبرز على
+
+339
+00:25:10,080 --> 00:25:14,820
+منطقة ال anticubital بحيث تبرزال .. الأوردة طبعا
+
+340
+00:25:14,820 --> 00:25:21,160
+don't place too tightly or leave on more than two
+
+341
+00:25:21,160 --> 00:25:25,040
+minutes يعني مش لازم انا اضغط يعني اعملها حزجة
+
+342
+00:25:25,040 --> 00:25:28,900
+كتير الternicate و بنفس الوقت مش لازم انا اخليها
+
+343
+00:25:28,900 --> 00:25:33,500
+مربوطة اكتر من .. من دقيقتين لإن ساعتهاراح يصير
+
+344
+00:25:33,500 --> 00:25:37,780
+فيه تأثير على العينة كمان شوية راح نحكي عنه، تمام؟
+
+345
+00:25:37,780 --> 00:25:43,360
+طيب فبالتالي مش لازم، عشان هيك أنا بحضر أدواتي،
+
+346
+00:25:43,360 --> 00:25:47,940
+تمام؟ prepare the blood collection assembly، بعدين
+
+347
+00:25:47,940 --> 00:25:53,870
+بربط الرابطة، تمام؟ طيببعدين the patient should
+
+348
+00:25:53,870 --> 00:25:59,350
+make a fist without bumping the hand بخليه ب .. ب
+
+349
+00:25:59,350 --> 00:26:02,810
+.. بحكي للمريض انه يسكر ايده و يضغط على ايده بس ما
+
+350
+00:26:02,810 --> 00:26:06,090
+ي .. ما .. ما يفتحاش و يسكرها without bumping انا
+
+351
+00:26:06,090 --> 00:26:09,670
+بديش هي يظل يفتح و يسكر فيها لأ بس يسكرها و يضغط
+
+352
+00:26:09,670 --> 00:26:14,410
+عليه على تسكرة ايده تمام؟ عشان تبرز الأوردة طبعا
+
+353
+00:26:14,410 --> 00:26:18,420
+يا بناتال .. ال .. الرابطة .. رابطة الternicate
+
+354
+00:26:18,420 --> 00:26:22,600
+إلها طريقة بحيث إنها تكون ماخدة شكل لفينكا، طبعا؟
+
+355
+00:26:22,600 --> 00:26:26,200
+على أساس إن أنا أسهل أفكها، إن شاء الله راح أعلمكم
+
+356
+00:26:26,200 --> 00:26:30,720
+إياها داخل المختبر و زي ما حكيتلكم هرح أصور video
+
+357
+00:26:30,720 --> 00:26:37,350
+و هذا ال .. ال video راح أبثلكم إياهبحيث انه او
+
+358
+00:26:37,350 --> 00:26:40,630
+يعني ارسلكم يا بحيث انه انتوا تشوفوا كيف عملية
+
+359
+00:26:40,630 --> 00:26:44,750
+السحب كيف انا بربط الرابطة كيف بحضر الأدوات كيف
+
+360
+00:26:44,750 --> 00:26:51,310
+بخلي ال blanger تبع الternic تصير سهل لين و كيف
+
+361
+00:26:51,310 --> 00:26:57,230
+بحس الوريد اي وريد بقرر ان انا اخدللسحب و بعد هيك
+
+362
+00:26:57,230 --> 00:27:01,750
+طبعا بعد ما اختار الوريد اللى بده سحب طبعا بحسه من
+
+363
+00:27:01,750 --> 00:27:06,970
+خلال بإصبع السبابة بعدين بعقم بحط على قطنة كحول و
+
+364
+00:27:06,970 --> 00:27:12,150
+بعقم بشكل دائري تمام؟ بعقم بشكل دائري يا بنات من
+
+365
+00:27:12,150 --> 00:27:16,930
+المركز و باخد ببعد على الأطراف يعني ب .. يعني
+
+366
+00:27:16,930 --> 00:27:20,570
+دائرة ضيقة و ببدأ أوسع أوسع أوسع لمن ما أصل
+
+367
+00:27:20,570 --> 00:27:26,020
+للأطراف و هكذاكيف يعني؟ لأنه أنا ساعتها بدي أخد ال
+
+368
+00:27:26,020 --> 00:27:28,780
+contaminated اللي موجودة في المركز عند منطقة
+
+369
+00:27:28,780 --> 00:27:35,240
+الوريد و أبعدها للأطراف، تمام؟ فإذا بقى الحركة
+
+370
+00:27:35,240 --> 00:27:40,300
+دائرية من .. تتسع من المركز للأطراف، هاي حركة
+
+371
+00:27:40,300 --> 00:27:44,120
+التعقيم أو ممكن تكون مسحة و خلاص، مارجعش أعيد تاني
+
+372
+00:27:45,050 --> 00:27:48,450
+تمام؟ ليش .. ليش أنا ب .. بعملش العكس، بعملش من
+
+373
+00:27:48,450 --> 00:27:51,430
+الأطراف للمركز، ساعتها أنا بكون .. قاعدة بلم كل ال
+
+374
+00:27:51,430 --> 00:27:55,010
+.. ال contamination و بحط هوايا في منطقة السحاب،
+
+375
+00:27:55,010 --> 00:28:00,800
+إذا بدي أنزح من الأطراف للمركز، تمام؟لو بدي أعمل
+
+376
+00:28:00,800 --> 00:28:03,200
+مسحة و أرجع أعيد عليها معناها أنا أخدت ال
+
+377
+00:28:03,200 --> 00:28:07,440
+contamination و رجعت عيدت عليها تاني، تمام؟ و رجعت
+
+378
+00:28:07,440 --> 00:28:12,120
+رجعتها مكانها، فهذا غلط، إذا بختار الوريد اللي أنا
+
+379
+00:28:12,120 --> 00:28:19,340
+بدي أسحب و بعقم بالمكان بشكل دائري و بعد هيكطبعا
+
+380
+00:28:19,340 --> 00:28:24,080
+بخلي الكحول يجف، طبعا اذا أنا استخدمت كحول كتير
+
+381
+00:28:24,080 --> 00:28:28,780
+لازم اجفف وانا بنفسي، ليش؟ يعني بدي استخدم قطعة
+
+382
+00:28:28,780 --> 00:28:35,540
+كحول .. قطعة كوتن معقمة وانشف فيها الكحوللكن لو
+
+383
+00:28:35,540 --> 00:28:40,520
+انا بدى .. طب استخدام الكحول كمية معقولة هو على
+
+384
+00:28:40,520 --> 00:28:43,880
+طول راح يتبخر و يعدى طب ليش انا بدى اجففه بنفسى
+
+385
+00:28:43,880 --> 00:28:47,400
+حكاية عشان ما يتبخر و ياخد معاه ماء و بالتالي يصير
+
+386
+00:28:47,400 --> 00:28:50,560
+في عندى hemoconcentration تمام؟
+
+387
+00:28:52,300 --> 00:28:56,540
+طيب طبعا prepare the patient arm using alcohol
+
+388
+00:28:56,540 --> 00:29:00,200
+swab it cleans in circular fashion بعقم في شكل
+
+389
+00:29:00,200 --> 00:29:05,200
+دائري وحكينا طبعا انه ال .. ببدأ من منطقة السحب و
+
+390
+00:29:05,200 --> 00:29:09,500
+ببدأ اوسع outward للخارج و عرفنا ليش عشان ال
+
+391
+00:29:09,500 --> 00:29:13,180
+contamination انا ابعدها عن مكان السحب grasp the
+
+392
+00:29:13,180 --> 00:29:19,700
+patient arm firmly بمسك ايد المريض ب ..ب .. يعني ب
+
+393
+00:29:19,700 --> 00:29:25,240
+.. بحيث أن أنا أكون مثبتاها تمام using your thumb
+
+394
+00:29:25,240 --> 00:29:30,220
+to draw the skin and anchor the vein ب .. ب ..
+
+395
+00:29:30,220 --> 00:29:36,180
+ممكن أنا يعني أمسك أيدي المريض أشد ال skin تبعه
+
+396
+00:29:36,180 --> 00:29:41,280
+بخلال ..الابهام تبعى عساس انه الوريد يظل ثابت و
+
+397
+00:29:41,280 --> 00:29:46,960
+يبرز اكتر و طبعا بدخل ال needle بدرجة .. بدرجة ما
+
+398
+00:29:46,960 --> 00:29:52,760
+بين 15 او بزاوية ما بين 15 ل 30 درجة و طبعا هدى هى
+
+399
+00:29:52,760 --> 00:29:57,520
+الزاوية المعتمدة و بعد هيك ببدأ ارجع ال blanker
+
+400
+00:29:57,520 --> 00:30:00,860
+لوراق طبعا مجرد ما انا دخلت فى الوريد يا بنات فى
+
+401
+00:30:00,860 --> 00:30:05,580
+منطقة ال hub تبعت ال needle بتظهر قطر قطر الدم
+
+402
+00:30:05,580 --> 00:30:11,720
+نرجع هنالما أركب ال needle على ال syringe بتأكد أن
+
+403
+00:30:11,720 --> 00:30:18,720
+ال bevel مقابل للتدريج، تمام؟ لما أدخل ال needle
+
+404
+00:30:18,720 --> 00:30:23,240
+داخل الوريد في زاوية ما بين خمستاشر لتلاتين إذا
+
+405
+00:30:23,240 --> 00:30:27,100
+أنا في الوريد في المكان الصحهنا فى منطقة ال hub فى
+
+406
+00:30:27,100 --> 00:30:31,040
+الجزء البلاستيكى الملون هتعلم عندى نقطة دم مجرد ما
+
+407
+00:30:31,040 --> 00:30:34,880
+شوفت نقطة دم معناه أنا فى الوريد الصح ببدأ اش اعمل
+
+408
+00:30:34,880 --> 00:30:38,020
+ارجع هذا البلانجر هذا البلانجر بيكون فى الداخل
+
+409
+00:30:38,020 --> 00:30:41,920
+ببدأ ارجعه لورا شوية شوية شوية شوية على أساس ايش
+
+410
+00:30:41,920 --> 00:30:48,170
+ان انا اسحب ال volume اللى بديه من الدمتمام هذا
+
+411
+00:30:48,170 --> 00:30:55,330
+بالنسبة للسحب اذا بفوت في زاوية من 15 ل 30 we
+
+412
+00:30:55,330 --> 00:30:58,870
+swiftly insert the needle through the skin and
+
+413
+00:30:58,870 --> 00:31:02,430
+into the lumen of the vein بفوت ال .. ال .. يعني
+
+414
+00:31:02,430 --> 00:31:08,220
+ال needleبخترق ال skin و بدخلها في الوريد و طبعا
+
+415
+00:31:08,220 --> 00:31:12,580
+the needle should be in the level in the bevel up
+
+416
+00:31:12,580 --> 00:31:17,600
+position يعني ال needle لازم يكون ال bevel مقابل
+
+417
+00:31:17,600 --> 00:31:23,320
+للتدريج و ال bevel اتجاهه لأعلى طبعا اتجاهه لأعلى
+
+418
+00:31:23,320 --> 00:31:26,860
+when the last tube is to be drawn is filling
+
+419
+00:31:26,860 --> 00:31:29,820
+removed aternity يعني انا بسحب .. اذا كنت بستخدم
+
+420
+00:31:29,820 --> 00:31:36,580
+طبعا نظام ال vacotainerو ب .. بحط .. ببدل ال tube
+
+421
+00:31:36,580 --> 00:31:40,600
+back عند آخر ال tube بفك ال tourniquet لو بستخدم
+
+422
+00:31:40,600 --> 00:31:44,320
+السيرنج يعني لما أحصل على ال volume اللي بديا و ال
+
+423
+00:31:44,320 --> 00:31:48,440
+needle لساتها جوا بفت .. بفك ال tourniquet بطلع ال
+
+424
+00:31:48,440 --> 00:31:52,460
+needle و بعدين remove the needle اذا بعد ما افك ال
+
+425
+00:31:52,460 --> 00:31:57,440
+tourniquet و طبعا اش بعمل يعني برجع لورا بطلع ال
+
+426
+00:31:57,440 --> 00:32:02,610
+needleو بعدك بحط قطرة قطن بحيث انه بخل المريض يضغط
+
+427
+00:32:02,610 --> 00:32:07,350
+على ايده بحيث انه يتوقف النظيف و مايصير فيه عنده
+
+428
+00:32:07,350 --> 00:32:10,510
+حاجة اسمها hematoma هنعرف اشي يعني hematoma كمان
+
+429
+00:32:10,510 --> 00:32:12,650
+شوية تمام
+
+430
+00:32:14,380 --> 00:32:17,600
+dispose of contaminated material supplies in
+
+431
+00:32:17,600 --> 00:32:21,080
+designed containers طبعا حكينا القطن الملوث بالدم
+
+432
+00:32:21,080 --> 00:32:25,440
+في السلة المكتوب عليها biohazard القطن اللي بس
+
+433
+00:32:25,440 --> 00:32:30,700
+عليه كحول و التغليف ال nylon اللي بيغلف ال syringe
+
+434
+00:32:30,700 --> 00:32:35,610
+و ال needle و الأشياء هذه كلها في السلة العاديةال
+
+435
+00:32:35,610 --> 00:32:39,230
+needle عشانها sharp بتنحط في ال sharp box، ال
+
+436
+00:32:39,230 --> 00:32:44,430
+syringe عشان كان فيها دم بحطها في السلة اللي
+
+437
+00:32:44,430 --> 00:32:48,350
+موجودة .. اللي محطوط عليها علامة biohazard، تمام؟
+
+438
+00:32:48,350 --> 00:32:53,790
+طيب mix and label أول ما بفضي ال .. ال .. ال ..
+
+439
+00:32:53,790 --> 00:33:00,570
+الدم من ال syringeالى ال tube المناسبة طبعا بعمل
+
+440
+00:33:00,570 --> 00:33:03,710
+بعدها بسكر ال tube بعمل mix gently يعني by
+
+441
+00:33:03,710 --> 00:33:09,950
+inversion يعني هيك بجلب ال .. ال tube by inversion
+
+442
+00:33:09,950 --> 00:33:16,230
+يعني لفجو لتحت و بعد هيك ب .. أعملها label مهم جدا
+
+443
+00:33:16,230 --> 00:33:20,710
+و ال patient لسه عندى بعمل labeling عشان ما أنسى و
+
+444
+00:33:20,710 --> 00:33:26,540
+ب .. ببعتها بعدها العينة في الحال الى المختبرطبعا
+
+445
+00:33:26,540 --> 00:33:30,560
+يا بنات، ليش أنا بلم .. ب .. بحس اتجاه الوريد؟
+
+446
+00:33:30,560 --> 00:33:35,060
+يعني ب .. ألمسه بالسبابة و بحاول أعرف اتجاهه؟ عشان
+
+447
+00:33:35,060 --> 00:33:38,560
+أنا لما بدي أفوت في الإبرة، أفوت و أنا في .. بكون
+
+448
+00:33:38,560 --> 00:33:42,420
+في نفس اتجاه المريض، في اتجاه الوريد، sorryفي نفس
+
+449
+00:33:42,420 --> 00:33:46,420
+اتجاه الوريد، لو الوريد هيك جاي، بروح بعكس اتجاه
+
+450
+00:33:46,420 --> 00:33:51,260
+الإبرة تبعتي بحيث إن أنا أجيله هيك، تمام؟ طيب، هي
+
+451
+00:33:51,260 --> 00:33:54,280
+الوريد .. لكن لو شايفين هنا هدي ال .. ال .. ال
+
+452
+00:33:54,280 --> 00:33:58,430
+needle مش في اتجاه الوريد، جاية معاكسهامعاها مدى
+
+453
+00:33:58,430 --> 00:34:02,290
+ضيق إنها تفوت داخل الوريد، طبعا؟ معاها مدى ضيق،
+
+454
+00:34:02,290 --> 00:34:05,090
+يعني ممكن إنها تيجي تخترق الوريد و تفوت من الجهة
+
+455
+00:34:05,090 --> 00:34:07,510
+التانية، تتخب الوريد من الجهة التانية، ساعتها الدم
+
+456
+00:34:07,510 --> 00:34:10,810
+الاشرح يصير يسرب من الجهة التانية و يتجمع تحت
+
+457
+00:34:10,810 --> 00:34:15,470
+الدم، تحت الاسكان، تحت الوريد، وهذا مشكلة، هلاجي
+
+458
+00:34:15,470 --> 00:34:20,180
+إيدي المريض انت فخدغلط لازم انا افوت في اتجاه
+
+459
+00:34:20,180 --> 00:34:23,460
+الوريد معايا ال needle شايفين طول و انا فايتة لو
+
+460
+00:34:23,460 --> 00:34:27,260
+فوتت اكتر لو فوتت اكتر بلاحظ انه ايش انا معايا
+
+461
+00:34:27,260 --> 00:34:33,440
+اتجاهها اكتر لحتى اني افوت فيه فبالتالي يعني بقلل
+
+462
+00:34:33,440 --> 00:34:36,900
+من خطر انه يحدث عند المريض انتفاخ في ايده او تسرب
+
+463
+00:34:36,900 --> 00:34:41,760
+في الدم تحت ال skin المعروف ب hematoma تمام؟
+
+464
+00:34:44,030 --> 00:34:48,750
+بحضر الأدوات تبعتي بربط ال tourniquet بحدد الوريد
+
+465
+00:34:48,750 --> 00:34:53,290
+بعقم تمام الوريد طبعا في عندي مسكة لل syringe
+
+466
+00:34:53,290 --> 00:34:56,350
+شايفين؟ شايفين؟ ال .. ال .. ال syringe و ال needle
+
+467
+00:34:56,350 --> 00:35:00,590
+السبابة بتكون عند ال hub و شايفين بإيدي الإبهام
+
+468
+00:35:00,590 --> 00:35:05,930
+أنا شادة ال skin بحيث أنه يبرز الوريد و بمسك ال ..
+
+469
+00:35:05,930 --> 00:35:09,530
+ب .. باقي ال .. الأصابع كلها هايت شايفينها كلها
+
+470
+00:35:09,630 --> 00:35:12,690
+بتكون موجودة تحت ال syringe و الإبهام هذا بيكون
+
+471
+00:35:12,690 --> 00:35:15,710
+موجود فوق ال syringe هورجيكم إياها ان شاء الله
+
+472
+00:35:15,710 --> 00:35:20,330
+هصوركم في ال video هتشوفوا بعدين بفوت في اتجاه
+
+473
+00:35:20,330 --> 00:35:24,550
+الوريد في زاوية معينة من خمستاشر لتلاتينلو كان يا
+
+474
+00:35:24,550 --> 00:35:29,130
+بنت الوريد deep بكبر الزاوية لو كان الوريد
+
+475
+00:35:29,130 --> 00:35:33,130
+superficial بظغر الزاوية تمام؟ مهم جدا هذه الشغله
+
+476
+00:35:33,130 --> 00:35:37,370
+و بعد هيك مجرد ما أفوت في الوريد و أتعلم عندي هنا
+
+477
+00:35:37,370 --> 00:35:43,090
+قطر الدم ببدأش أعمل أرجع البلانجر لوره بسحب بيد
+
+478
+00:35:43,090 --> 00:35:48,230
+التانية البلانجر و ببدأ يرعبي عندي ال blood في ال
+
+479
+00:35:48,230 --> 00:35:52,540
+syringeمجرد ما خلصت أخدت الحجم اللي بدياه أول إيش
+
+480
+00:35:52,540 --> 00:35:55,640
+بعمله بربط ب .. ب .. ب .. ب .. ب .. ب .. ب .. ب ..
+
+481
+00:35:55,640 --> 00:35:55,880
+ب .. ب .. ب .. ب .. ب .. ب .. ب .. ب .. ب .. ب ..
+
+482
+00:35:55,880 --> 00:35:58,300
+ب .. ب .. ب .. ب .. ب .. ب .. ب .. ب .. ب .. ب ..
+
+483
+00:35:58,300 --> 00:35:58,780
+ب .. ب .. ب .. ب .. ب .. ب .. ب .. ب .. ب .. ب ..
+
+484
+00:35:58,780 --> 00:35:58,800
+ب .. ب .. ب .. ب .. ب .. ب .. ب .. ب .. ب .. ب ..
+
+485
+00:35:58,800 --> 00:35:59,100
+ب .. ب .. ب .. ب .. ب .. ب .. ب .. ب .. ب .. ب ..
+
+486
+00:35:59,100 --> 00:35:59,860
+ب .. ب .. ب .. ب .. ب .. ب .. ب .. ب .. ب .. ب ..
+
+487
+00:35:59,860 --> 00:35:59,860
+ب .. ب .. ب .. ب .. ب .. ب .. ب .. ب .. ب .. ب ..
+
+488
+00:35:59,860 --> 00:36:00,800
+ب .. ب .. ب .. ب .. ب .. ب .. ب .. ب .. ب .. ب ..
+
+489
+00:36:00,800 --> 00:36:11,140
+ب .. ب .. ب .. ب .. ب .. ب .. ب .. ب ..
+
+490
+00:36:18,780 --> 00:36:22,560
+تمام؟ علشان لو أنا عملت العكس، لو طلعت من الواليد
+
+491
+00:36:22,560 --> 00:36:26,400
+و لسه فيه ضغط، لسه فيه ال .. الternicات مربوطة،
+
+492
+00:36:26,400 --> 00:36:31,560
+راح الدم .. الاجتدام بيندفع اش .. الى خارج ال
+
+493
+00:36:31,560 --> 00:36:34,680
+skin، راح يصير في زي ال bleeding و جزء منه كمان
+
+494
+00:36:34,680 --> 00:36:37,460
+هيعبي تحت ال skin و يعملي انتفاخ يعني هيعملي
+
+495
+00:36:37,460 --> 00:36:41,900
+hematoma، تمام؟هذه شغلة الشغلة التانية هنا الترتيب
+
+496
+00:36:41,900 --> 00:36:45,860
+أيضا إن أنا أطلع ال needle بعدين أحط القطنة، تخيل
+
+497
+00:36:45,860 --> 00:36:50,520
+لو أنا ال needle جوا حطت القطنة و دغطت، يعني أنا
+
+498
+00:36:50,520 --> 00:36:53,200
+دغطت على ال needle اللي هو جوا، إذا أنا سببت ألم
+
+499
+00:36:53,200 --> 00:36:56,980
+للمريض و هي الإبرة جواته، دغطت عليها، فغلط، لازم
+
+500
+00:36:56,980 --> 00:37:02,740
+أطلع بعدين أحط القطة و أخليه، اضغطتمام خلينا نشوف
+
+501
+00:37:02,740 --> 00:37:08,800
+ال .. ال discussion يعني المناقشة أو النقاط المهمة
+
+502
+00:37:08,800 --> 00:37:15,700
+المتعلقة بعملية جمع العين الدم من ال van تمام؟ ال
+
+503
+00:37:15,700 --> 00:37:20,180
+van punctureأول إشي there are certain area are to
+
+504
+00:37:20,180 --> 00:37:23,120
+be avoided when choosing a site of vent puncture
+
+505
+00:37:23,120 --> 00:37:26,860
+يعني أنا لما بدأ أختار مكان الوريد بدي أبعد عن هاي
+
+506
+00:37:26,860 --> 00:37:30,920
+الشغلات أول إشي طبعا هذا أيضا بيكون موضوع السؤال
+
+507
+00:37:30,920 --> 00:37:37,960
+extensive scarيعني لو كان فيه ندوب أو حروق أو أثر
+
+508
+00:37:37,960 --> 00:37:42,380
+جراحة، أثر جروح موجود في مكان الوريدبة بعيد عنه،
+
+509
+00:37:42,380 --> 00:37:47,140
+تمام؟ و أيضا إذا كان في ال .. في الإيد المحاذية
+
+510
+00:37:47,140 --> 00:37:54,030
+لما كان صار له mastectomy، يعني مثلافي الأماكن
+
+511
+00:37:54,030 --> 00:38:00,850
+اللي يعني لو الست صار عندها استقصال لسدي مثلا،
+
+512
+00:38:00,850 --> 00:38:05,170
+السدي الأيمن ممنوع أنا أسحب من الإيد اليمنى، الإيد
+
+513
+00:38:05,170 --> 00:38:07,610
+المحادية للمكان اللي صار domestic to me صار
+
+514
+00:38:07,610 --> 00:38:13,610
+الاستقصال لكتلة ممنوع أسحب منهاتمام؟ عشان هيك
+
+515
+00:38:13,610 --> 00:38:16,890
+بتلاحظوا ان مرضى ال .. ال .. ال breast cancer
+
+516
+00:38:16,890 --> 00:38:22,330
+الناس اللي تعرضوا لاستقصال بيلابسهم دايما اسورة في
+
+517
+00:38:22,330 --> 00:38:26,630
+إيدهم المحادية لمنطقة الاستقصال و مكتوب على هذه
+
+518
+00:38:26,630 --> 00:38:31,280
+الإيد don't touch my armتمام؟ يعني لا تلمس إيدي
+
+519
+00:38:31,280 --> 00:38:34,340
+عشان هدول الناس لو تعرضوا لإغماء أو لو نكلوا إلى
+
+520
+00:38:34,340 --> 00:38:37,560
+المستشفى أو لو كانوا مش بواعيهم و إجينا بنسحب منهم
+
+521
+00:38:37,560 --> 00:38:41,220
+عينة مجرد بنشوف هذه الإسورة اللي مكتوب عليها don't
+
+522
+00:38:41,220 --> 00:38:44,360
+touch my arm راح أعرف إنه في هذه المنطقة أو في
+
+523
+00:38:44,360 --> 00:38:49,480
+المنطقة المحادية صار في mastectomy وبالتالي مش
+
+524
+00:38:49,480 --> 00:38:52,830
+ممنوع أنا أسحب منها عينة دمطب لو سحبت عين الدم إيش
+
+525
+00:38:52,830 --> 00:38:57,290
+بيصير؟ بيصير في عند المريض lymphedema بتنتفخ الغدط
+
+526
+00:38:57,290 --> 00:39:01,670
+اللينفوية عنده بيصير في عنده آلام كبيرة، تمام؟ و
+
+527
+00:39:01,670 --> 00:39:06,910
+أيضا المنطقة اللي فيها hematoma لو شخص من .. يعني
+
+528
+00:39:06,910 --> 00:39:11,390
+انسحبله عين أصابقا و صار في عنده hematoma، تمام؟
+
+529
+00:39:11,390 --> 00:39:16,500
+يعني انتفاخفي منطقة السحب نتيجة تراكم الدم تحت ال
+
+530
+00:39:16,500 --> 00:39:20,400
+skin هذا برضه كمان بتجنب أن أنا أسحبله من منطقة ال
+
+531
+00:39:20,400 --> 00:39:25,580
+hematoma لإنه هسحب دم مكسر هسحب دم مكسر ممنوع أن
+
+532
+00:39:25,580 --> 00:39:29,300
+أنا أسحب في المنطقة هذه بسحب بعدها أو قبلها أو من
+
+533
+00:39:29,300 --> 00:39:32,640
+الإيد التانية بستنى عليها للطيب ال hematoma يا
+
+534
+00:39:32,640 --> 00:39:35,620
+بنات بالطيب يعني بتاخدلها فترة من أسبوع لعشر أيام
+
+535
+00:39:35,620 --> 00:39:42,150
+و بتروحيعني دم يعني ترعكب تحت ال skin الجسم بتخلص
+
+536
+00:39:42,150 --> 00:39:47,140
+منه بعد فترةتمام؟ طيب في .. أيضا في منطقة ال
+
+537
+00:39:47,140 --> 00:39:49,900
+intravenous therapy لو الشخص بياخد blood
+
+538
+00:39:49,900 --> 00:39:55,840
+transfusion عم ننقله دم بالوريد أو عم ننقله سائل
+
+539
+00:39:55,840 --> 00:39:59,980
+محلول فبالتالي ساعتها يأم هيكون الدم إذا بننقله
+
+540
+00:39:59,980 --> 00:40:03,940
+محلول يعني الدم مخفف في هاي المنطقة تمام؟ إذا عم
+
+541
+00:40:03,940 --> 00:40:07,780
+ننقله دم يعني وحدة دم معناه الدم اللي في المنطقة
+
+542
+00:40:07,780 --> 00:40:12,030
+هذه مش دمه لسه تمام؟ هذا الدم اللي انتقلهفلازم
+
+543
+00:40:12,030 --> 00:40:16,330
+يصير في circulation علشان انا ا .. يختلط الدم في
+
+544
+00:40:16,330 --> 00:40:21,730
+بعض و .. و يصير يعني العينة معبرةفبالتالي .. طب ..
+
+545
+00:40:21,730 --> 00:40:24,910
+فبالحالة هذه أنا المنطقة هذه مش لازم أسحب منها،
+
+546
+00:40:24,910 --> 00:40:30,890
+المنطقة اللي فيها canula لمحلول أو لوح .. نقل وحت
+
+547
+00:40:30,890 --> 00:40:35,690
+الدم طب إيش بدي أعمل لو كان مثلا الشخص هذا يا إما
+
+548
+00:40:35,690 --> 00:40:39,850
+بنجي ل .. ب .. بسحبله من الإيد التانية أو إيش بعمل
+
+549
+00:40:39,850 --> 00:40:47,930
+لو الشخص هذا عم بياخد سائل؟محلول وريدي
+
+550
+00:40:47,930 --> 00:40:54,510
+أول إشي turn off the IV بسكر ال .. ال flow بسكر ال
+
+551
+00:40:54,510 --> 00:40:58,290
+cannula بحيث أنه مايفوتله IV لمدة دقيقتين على
+
+552
+00:40:58,290 --> 00:41:03,090
+الأقل قبل ال pen punctureتمام؟ و بحط الternicate
+
+553
+00:41:03,090 --> 00:41:08,710
+تحت مكان ال IV site مكان ما هو داخل ال cannula و
+
+554
+00:41:08,710 --> 00:41:13,010
+مكان ما دخل المحلول انا بربط الternicate لتحتها
+
+555
+00:41:13,010 --> 00:41:17,430
+ليش؟ لإنه يا بنات خليني مثلا أشرحلكم على الصورة
+
+556
+00:41:17,430 --> 00:41:22,910
+هنا على صورة الأوردة نرجع هنا نفرض إنه هنا في
+
+557
+00:41:22,910 --> 00:41:26,470
+cannula تمام؟ بسكر أول إشي ال cannula بحيث إنه
+
+558
+00:41:26,470 --> 00:41:33,330
+يتوقف ال IVو تاني شغلة يا بنات إيش بعمل؟ بركب ال
+
+559
+00:41:33,330 --> 00:41:37,150
+tourniquet لتحت، هلأ المحلول هو رايح فوق، هيك
+
+560
+00:41:37,150 --> 00:41:41,150
+رايح، في هذا الاتجاه، تمام؟ فالدم اللي هنا مش
+
+561
+00:41:41,150 --> 00:41:44,170
+مختلف بالمحلول، عشان هيك أنا بحط ال tourniquet تحت
+
+562
+00:41:44,170 --> 00:41:49,070
+و بسحب من الأوردة اللي تحت، المكان ال IV، تمام؟
+
+563
+00:41:49,070 --> 00:41:54,810
+هاي معنى كلمة apply the tourniquet below the IV
+
+564
+00:41:54,810 --> 00:42:00,090
+siteselect a vein بدور على vein كويس و ريد منيح و
+
+565
+00:42:00,090 --> 00:42:05,850
+مناسب لسحب العينة و باجري ال vein puncture بسحب
+
+566
+00:42:05,850 --> 00:42:10,250
+أول خمسة أمال و بتخلص منهم بعدين بسحب العينة اللي
+
+567
+00:42:10,250 --> 00:42:15,090
+بديها كمان كل هذه إجراءات بعملها علشان أتأكد أن
+
+568
+00:42:15,090 --> 00:42:19,990
+أنا باخد عينة ممثلة representative sample عينة لما
+
+569
+00:42:19,990 --> 00:42:23,830
+أحصل يعني لما أعمل عليها بحوصاته أحصل على نتائج
+
+570
+00:42:23,830 --> 00:42:29,660
+تكون النتائج معبرة عن وضع المريضتمام hematoma إيش
+
+571
+00:42:29,660 --> 00:42:33,780
+يعني hematoma؟ حكينا هو انتفاخ في منطقة السحب
+
+572
+00:42:33,780 --> 00:42:40,380
+تمام؟ نتيجة إيش؟ نتيجة إن ال blood accumulate ..
+
+573
+00:42:40,380 --> 00:42:46,260
+blood accumulate under the skin تمام؟ ف .. هي تحدث
+
+574
+00:42:46,260 --> 00:42:49,900
+form under the skin adjacent to the puncture site
+
+575
+00:42:49,900 --> 00:42:54,280
+تحدث تحت ال skin في منطقة إيش؟ ال .. ال .. السحب
+
+576
+00:42:54,800 --> 00:42:58,160
+release طبعا اذا اول ما شوفها اول ما شوف انه صار
+
+577
+00:42:58,160 --> 00:43:01,100
+في انتفاخ في هذه المنطقة يعني الدم اتجمع في هذه
+
+578
+00:43:01,100 --> 00:43:04,360
+المنطقة اول اشي بعمله release the tourniquet
+
+579
+00:43:04,360 --> 00:43:08,320
+immediately برفع ال tourniquet immediately و بسحب
+
+580
+00:43:08,320 --> 00:43:13,020
+ال needle و بخلي المريض يحط قطنه و يضغط عليها طب
+
+581
+00:43:13,020 --> 00:43:16,060
+ايش اسباب ال hematoma ايش اللي بيخلي الدم يتجمع
+
+582
+00:43:16,060 --> 00:43:21,800
+تحت ال skin هنا يطلع من الوريد و يفوت داخل ال skin
+
+583
+00:43:23,000 --> 00:43:26,920
+يعني يتجمع ما بين .. تحت ال skin أول إشي the
+
+584
+00:43:26,920 --> 00:43:29,780
+needle may have gone through the vein يعني ال ..
+
+585
+00:43:29,780 --> 00:43:33,700
+ال .. ال .. ال needle اخترقت الوريد، هاي الوريد
+
+586
+00:43:33,700 --> 00:43:36,640
+اللي لونه أحمر فاتت فيه و طلعت من الجهة التانية،
+
+587
+00:43:36,640 --> 00:43:38,800
+يعني أنت فاقبتي من الجهة التانية، الجهة التانية
+
+588
+00:43:38,800 --> 00:43:42,440
+هدى، راح تصير تمزف و يتجمع الدم تحت ال skin، إذا
+
+589
+00:43:42,440 --> 00:43:46,800
+سي .. راح يصير انتفاخ زي هيكremove the needle
+
+590
+00:43:46,800 --> 00:43:50,440
+before removing the tourniquet لو أنا رفعت ال
+
+591
+00:43:50,440 --> 00:43:53,760
+needle قبل ال tourniquet حكينا هيصير أول إشي
+
+592
+00:43:53,760 --> 00:43:58,760
+bleeding وجزء من الدم هيتحبس تحت ال skin فهيصير في
+
+593
+00:43:58,760 --> 00:44:03,700
+عندي hematoma تمام؟ عشان هيك حكينا الترتيب مهم
+
+594
+00:44:03,700 --> 00:44:07,030
+برفع ال tourniquet بالأول بعدين ال needlethe
+
+595
+00:44:07,030 --> 00:44:12,130
+needle may not fully penetrate the uppermost wall
+
+596
+00:44:12,130 --> 00:44:18,330
+of vein يعني لو انا ال .. ال .. ال needle لو كانت
+
+597
+00:44:18,330 --> 00:44:24,070
+يعني زي ما انتوا شايفين هنا مش مخترقة بشكل كامل
+
+598
+00:44:24,070 --> 00:44:27,210
+الوريد هذا ال bevel نصه جاي .. هاي ال bevel
+
+599
+00:44:27,210 --> 00:44:31,590
+شايفينه الجزء المائل هذا اللي بزاوية جاي فتحت ال
+
+600
+00:44:31,590 --> 00:44:37,250
+needle طبعاجزء هذا ال bevel زي ما انتوا شايفين جزء
+
+601
+00:44:37,250 --> 00:44:39,910
+من ال bevel هذا مخترق الوريد والجزء التاني برا
+
+602
+00:44:39,910 --> 00:44:43,130
+يعني هلأ الدم اللي في الوريد بيجي بعب في ال bevel
+
+603
+00:44:43,130 --> 00:44:47,670
+بيطلع من الجزء اللي برا وبيتحوش تحت ال skin يعني
+
+604
+00:44:47,670 --> 00:44:50,770
+بتكون تحت ال skin هنا accumulate under the skin
+
+605
+00:44:50,770 --> 00:44:56,970
+وبيعمللي انتفاخ اذا hematoma تمام؟ فهذا ايضا احد
+
+606
+00:44:56,970 --> 00:45:03,090
+الأسباب و ايضا failureto apply pressure إذا أنا
+
+607
+00:45:03,090 --> 00:45:06,690
+حكيت للمريض اضغط بعد ما خلصت إذا أنا سحبت بطريقة
+
+608
+00:45:06,690 --> 00:45:10,630
+صح و حكيت لأنا المريض اضغط و المريض ماضغط منيح
+
+609
+00:45:10,630 --> 00:45:14,150
+معناها أيضا كمان ممكن أن الدم يتخلص من تحت ال skin
+
+610
+00:45:14,150 --> 00:45:21,610
+و يعمل لي hematomaحاكي هنا إنه ال .. إما إنه ال ..
+
+611
+00:45:21,610 --> 00:45:25,990
+ال .. الإبرة بتخترق الويد بشكل كامل أو إنها تخترق
+
+612
+00:45:25,990 --> 00:45:32,990
+partially و .. أو إنه أرفع الternicate قبل ما أرفع
+
+613
+00:45:32,990 --> 00:45:36,710
+ال needle .. sorry بعد ما أرفع ال needle، يعني ال
+
+614
+00:45:36,710 --> 00:45:40,090
+needle قبل، هذا بسبب لي hematoma الصح إنه أرفع
+
+615
+00:45:40,090 --> 00:45:45,010
+الternicate بالأولتمام هى احد .. هى .. هذا هو ال
+
+616
+00:45:45,010 --> 00:45:47,970
+correct position هى الوريد جاي هيك و انا جاية في
+
+617
+00:45:47,970 --> 00:45:54,170
+اتجاهه بزاوية معينة و بسحب ال blood لو انا جيت و
+
+618
+00:45:54,170 --> 00:45:57,790
+اخترقته هيصير في عندي ترق .. ترق .. هي .. الدم
+
+619
+00:45:57,790 --> 00:46:01,170
+هيطلع من الوريد و يتركم تحت ال skin و يعمللي
+
+620
+00:46:01,170 --> 00:46:05,510
+hematoma اذا انا اخترقت بشكل partially يعني جزء من
+
+621
+00:46:05,510 --> 00:46:08,750
+ال pivotal في الوريد و جزء منه تحت ال skin معناه
+
+622
+00:46:08,750 --> 00:46:12,700
+ان الدم هيجي يعبي و يطلع من الجهة التانيةو يتراكم
+
+623
+00:46:12,700 --> 00:46:17,600
+تحت ال skin و أيضا هيسبب لهما طبعا إذا أنا جيت ال
+
+624
+00:46:17,600 --> 00:46:22,860
+.. ال .. ال bevel resting on vein wall إذا جيت على
+
+625
+00:46:22,860 --> 00:46:27,880
+منطقة انحناق أو انحناق للوريد و جيت سحبت ممكن أنه
+
+626
+00:46:27,880 --> 00:46:31,700
+ال .. ال .. ال bevel هذا يلتصق بجدار الوريد و
+
+627
+00:46:31,700 --> 00:46:37,520
+يتوقف سحب الدم تمام؟ و إذا كان أنا دخلت .. دخلت ال
+
+628
+00:46:37,520 --> 00:46:45,260
+needle في مكانطبعا في .. في مكان تفرع أو في وريد
+
+629
+00:46:45,260 --> 00:46:49,120
+يعني من وريد ضيق إلى رفيع ممكن يتوقف سحب الدم يعني
+
+630
+00:46:49,120 --> 00:46:53,780
+يسحب شوية و بعدين يتوقف فهذا برضه خطأ الحل أن أنا
+
+631
+00:46:53,780 --> 00:46:57,600
+أرجع لورا شوية أطلع ال needle شوية أرجع لورا
+
+632
+00:46:57,600 --> 00:47:04,140
+فأخليها set في الوريد العميق السميك و أسحب عين في
+
+633
+00:47:04,140 --> 00:47:10,340
+الدم تمام؟لو أنا جيت رفع .. طلعت البلانجر، رجعت
+
+634
+00:47:10,340 --> 00:47:15,520
+البلانجر تابع ال needle بقوة سحبته لورا، راح يدفع
+
+635
+00:47:15,520 --> 00:47:19,160
+الدم في داخل ال syringe بقوة و هيصير في عندي
+
+636
+00:47:19,160 --> 00:47:22,420
+انقباض في الوريد أو لو حتى كمان طولت في رابطة
+
+637
+00:47:22,420 --> 00:47:25,620
+الternicate ممكن يصير في انقباض في الوريد و يتوقف
+
+638
+00:47:25,620 --> 00:47:30,750
+الدمand يعني هيسحب شوية من الدم و يتوقف بعد هيك
+
+639
+00:47:30,750 --> 00:47:34,950
+هيبطل يطلع دم فالأفضل أن أنا أفك الternicate علشان
+
+640
+00:47:34,950 --> 00:47:37,010
+يرجع الوريد أشماله
+
+641
+00:47:39,010 --> 00:47:43,410
+يبطل منقبض يرجع يفتح و يرجع يصير في مسار للدم و
+
+642
+00:47:43,410 --> 00:47:48,410
+أرجع أسحب عينة الدم تمام هذا بالنسبة لشكل أو
+
+643
+00:47:48,410 --> 00:47:53,090
+position أو ضاع ال needle داخل الوريد عشان أمنع ال
+
+644
+00:47:53,090 --> 00:47:58,330
+hemolysis أمنع تكسر الدم أول إشي لازم لما أفضل ال
+
+645
+00:47:58,330 --> 00:48:03,790
+blood في ال .. ال tube أعمل mixing لل tube تمام
+
+646
+00:48:03,790 --> 00:48:10,520
+mixing لل blood gently تماممن خمسة لعشر مرات مع ال
+
+647
+00:48:10,520 --> 00:48:12,840
+anticoagulant اللي موجود فيها اللي هو ال additive
+
+648
+00:48:12,840 --> 00:48:16,780
+اللي موجود داخل ال tube avoid drawing blood from
+
+649
+00:48:16,780 --> 00:48:19,960
+hematoma ممنوع أسحب من مكان فيه hematoma حكيتلكم
+
+650
+00:48:19,960 --> 00:48:23,820
+لإني راح أحصل على دم مكسر hemolized blood avoid
+
+651
+00:48:23,820 --> 00:48:28,160
+drawing the plunger back to forcely برضه كمان لما
+
+652
+00:48:28,160 --> 00:48:32,760
+أنا أجي أسحب ال plunger بقوة الدم هيجي يندفع بقوة
+
+653
+00:48:32,760 --> 00:48:35,600
+على حفة ال needle أو على فتحة ال needle اللي هي
+
+654
+00:48:35,600 --> 00:48:40,530
+المكان اللي .. اللي ..اللي بزاوية هدا جاي ال bevel
+
+655
+00:48:40,530 --> 00:48:45,050
+ه .. الخلايا هتتكسر عند هذا الجزء او نتيجة
+
+656
+00:48:45,050 --> 00:48:49,490
+اندفاعها بسرعة داخل ال syringe ايضا هتتكسر make
+
+657
+00:48:49,490 --> 00:48:52,830
+sure the paint puncture is dry مافيش بقايا كحول
+
+658
+00:48:52,830 --> 00:48:56,350
+على مكان ال paint puncture عشان اختلاط الكحول مع
+
+659
+00:48:56,350 --> 00:49:00,110
+ال blood هيعمللي hemolysis ايضاو avoid probing
+
+660
+00:49:00,110 --> 00:49:05,570
+ممنوع انا اربط .. اضرب على مكان الوريد او اخد او
+
+661
+00:49:05,570 --> 00:49:08,830
+اصحب من منطقة فيها trauma فيها جرح او فيها ضربة او
+
+662
+00:49:08,830 --> 00:49:13,390
+فيها كدمة و طبعا ال massaging ان انا اعمل
+
+663
+00:49:13,390 --> 00:49:18,290
+massaging قوي او اعصر او انا انه انا يعني اضرب او
+
+664
+00:49:18,290 --> 00:49:22,770
+اربط على المكان هذا ايضا ممكن يحفظ حدوط ال homoses
+
+665
+00:49:22,770 --> 00:49:27,490
+تمام؟ طيبإذا أنا ربطت ال tourniquet أكتر من two
+
+666
+00:49:27,490 --> 00:49:31,750
+minutes إيش هيصير؟ أول حاجة أنا .. أنا لما أربط ال
+
+667
+00:49:31,750 --> 00:49:34,810
+tourniquet يعني أنا عاملة ضغط حابسة الدم داخل
+
+668
+00:49:34,810 --> 00:49:40,110
+الإيد أكتر من two minutes، تمام؟ يعني مانع اختلاط
+
+669
+00:49:40,110 --> 00:49:44,330
+الدم اللي موجود في منطقة السحب مع باقي الدم اللي
+
+670
+00:49:44,330 --> 00:49:47,330
+موجود في ال circulation طالما أنا منعت اختلاطه
+
+671
+00:49:47,330 --> 00:49:50,790
+يعني الدم هذا اتركز صار .. صار فيه
+
+672
+00:49:50,790 --> 00:49:55,560
+hemoconcentrationفبالتالي يعني انه كل إشي .. كل
+
+673
+00:49:55,560 --> 00:49:59,940
+إشي صارله increased concentration تمام؟ إذا ال
+
+674
+00:49:59,940 --> 00:50:03,780
+hemoconcentration هي تعريفها تيجي بالمصطلح is
+
+675
+00:50:03,780 --> 00:50:07,000
+defined as increased concentration of larger
+
+676
+00:50:07,000 --> 00:50:11,000
+molecules and formed element in the blood or non
+
+677
+00:50:11,000 --> 00:50:13,260
+-filtrable element تمام؟
+
+678
+00:50:17,100 --> 00:50:21,340
+طبعا ايضا كمان ان انا اربط ال tourniquet اكتر من
+
+679
+00:50:21,340 --> 00:50:25,040
+two minutes حكينا لما اجي اسحب ممكن ينقبض الوريد
+
+680
+00:50:25,040 --> 00:50:29,440
+يصير فيه van collapse و يتوقف السحب يعني بصحب شوية
+
+681
+00:50:29,440 --> 00:50:32,340
+دم بلاجي و جافقاش فقاش الفجأة اه ممكن انا اكون
+
+682
+00:50:32,340 --> 00:50:36,080
+طولت على ال tourniquet تشوف ال كيميا هلاحظ انه صار
+
+683
+00:50:36,080 --> 00:50:38,800
+في اذرقاق في المنطقة نتيجة ان ال oxygen delivery
+
+684
+00:50:38,800 --> 00:50:42,360
+او ال oxygenقل في المنطقة هذه لإنه أنا محبسة يعني
+
+685
+00:50:42,360 --> 00:50:46,580
+أنا ضاغطة تخيل أنت شخص .. يعني أحيانا الطفل لما
+
+686
+00:50:46,580 --> 00:50:51,380
+بيجي بيكون لف على إيده خيط أو مغيطة أو ضاغط عليها
+
+687
+00:50:51,380 --> 00:50:55,500
+بتحس إنه المنطقة إزرقت لنتيجة إن هو إيش اتحبس الدم
+
+688
+00:50:55,500 --> 00:50:59,050
+فهي نفس الفكرةهذه معناه كلمة tissue ischemia يعني
+
+689
+00:50:59,050 --> 00:51:03,830
+إذ رقاغ ونقص في ال oxygen تمام؟ air must never be
+
+690
+00:51:03,830 --> 00:51:07,950
+pumped into a van because a few cubic centimeter
+
+691
+00:51:07,950 --> 00:51:13,260
+of air pumped into a van could cause deathلازم انا
+
+692
+00:51:13,260 --> 00:51:19,460
+اتأكد انه ال syringe مسكرة أثناء سحب العينة يعني
+
+693
+00:51:19,460 --> 00:51:23,440
+انا صح بلينها بحرك البلانجر مرة مرتين تلات عشان
+
+694
+00:51:23,440 --> 00:51:28,020
+ألينها بعدين بسكرها منيع بفرغها من الهواء جاب اللي
+
+695
+00:51:28,020 --> 00:51:31,320
+أبدأ في السحب لإنه اذا انا حقنت هوا في الوريد هذا
+
+696
+00:51:31,320 --> 00:51:37,590
+قاتل تمام؟ ممنوعblanger of syringe mustn't be
+
+697
+00:51:37,590 --> 00:51:41,710
+pulled back with too force لما انا .. انا .. لما
+
+698
+00:51:41,710 --> 00:51:45,830
+افوت في الوريد ممنوع ا .. ارجع الblanger بقوة تبع
+
+699
+00:51:45,830 --> 00:51:49,930
+ال syringe ليش؟ اول حاجة حكينا بيسبب homolysis او
+
+700
+00:51:49,930 --> 00:51:54,150
+ممكن انه يسبب ash vein collapse حكينا كمان طبعا؟
+
+701
+00:51:54,240 --> 00:52:00,780
+ممكن انه ايضا الجدار تبع الوريد يلتصق بال bevel و
+
+702
+00:52:00,780 --> 00:52:05,220
+يتوقف الدم، اذا van collapse, void ممكن يصير في
+
+703
+00:52:05,220 --> 00:52:09,860
+homolescence، ممكن انه ال wall of van، جدار الوريد
+
+704
+00:52:09,860 --> 00:52:16,060
+يعني ينسحب، يصيرله pull او يصيرله القصاق adhere to
+
+705
+00:52:16,060 --> 00:52:21,650
+the bevel of needle فبالتالي يتوقف السحب الدمطيب
+
+706
+00:52:21,650 --> 00:52:26,230
+لو انا في .. لو انا بديت اسحب دم و توقف الدم فجأة
+
+707
+00:52:26,230 --> 00:52:29,630
+يعني انا طلعت شوية دم بس مش الكمية الكافية، توقف
+
+708
+00:52:29,630 --> 00:52:32,790
+السحب، صارت ب .. برجع بالبلانجر ماعم بيعطيني ال ..
+
+709
+00:52:32,790 --> 00:52:38,710
+يعني more blood، تمام؟إيش بدي أعمل ساعتها؟ أول إشي
+
+710
+00:52:38,710 --> 00:52:42,950
+بدي أحرك ممكن أنا أكون يعني اتحركت معايا ال needle
+
+711
+00:52:42,950 --> 00:52:46,910
+و ال syringe يعني فوتت بعمق أكتر أو غيرت الزاوية
+
+712
+00:52:46,910 --> 00:52:51,990
+أو غيرت الاتجاه، تمام؟ فساعتها أنا بدي أظبط ال ..
+
+713
+00:52:51,990 --> 00:52:55,550
+ال .. ال position تبع ال needle يعني بدي أحركها
+
+714
+00:52:55,550 --> 00:53:01,290
+إما للأمام تمام؟ أو أرجعها للخلفو في كل مرة أجرب
+
+715
+00:53:01,290 --> 00:53:04,950
+أنه أرجع ال blender أشوف هو هيرجع يسحب ولا لا،
+
+716
+00:53:04,950 --> 00:53:11,010
+بكبر الزاوية، ب .. ب .. ب .. أصغرها، بشوف يعني ب
+
+717
+00:53:11,010 --> 00:53:15,190
+.. ب .. ب .. بلفها يمين، بلفها يسار، بحيث أنه يعني
+
+718
+00:53:15,190 --> 00:53:18,790
+أظبط أيضا الاتجاه، اذا أول حاجة change the
+
+719
+00:53:18,790 --> 00:53:22,470
+position of the needlemove it forward بحركها
+
+720
+00:53:22,470 --> 00:53:28,390
+للأمام or move it backward او للخلف اسحبها للخلف
+
+721
+00:53:28,390 --> 00:53:32,550
+يعني اطلعها شوي بحيث انه ممكن ممكن انا اخترقت
+
+722
+00:53:32,550 --> 00:53:38,050
+زيادة عن ال zone ممكن اكون adjust the angle اظبط
+
+723
+00:53:38,050 --> 00:53:41,190
+الزاوية ارفع الزاوية او يعني اكبر الزاوية او
+
+724
+00:53:41,190 --> 00:53:45,650
+اصغرها ممكن يكون صار في عندى van collapse فالافضل
+
+725
+00:53:45,650 --> 00:53:51,590
+ان انا اعمل افتح يعني افك ال tourniquet تمامو ..
+
+726
+00:53:51,590 --> 00:53:54,950
+أو ممكن أرجع أدور على الوريد مرة تانية عشان أشوف
+
+727
+00:53:54,950 --> 00:53:59,710
+وين اتجاهه و أدخله من خلال نفس الاتجاه هذه الشغلات
+
+728
+00:53:59,710 --> 00:54:05,650
+كلها هتلاحظوا أنه .. هتلاحظوني بعلمكم إياها أثناء
+
+729
+00:54:05,650 --> 00:54:11,260
+ال .. الممارس العمليةلما مثلا انت تجربى مع زميلتك
+
+730
+00:54:11,260 --> 00:54:15,800
+و ت .. تفاجأى انه .. انه سحبتى منها شوية blood
+
+731
+00:54:15,800 --> 00:54:19,740
+بعدين توقف الدم ايش المشكلة بصيل اهاجى جنبك و
+
+732
+00:54:19,740 --> 00:54:24,920
+احكىلك رجعيها لورا شوية جربى اصحبي ماصحبش بحكيلك
+
+733
+00:54:24,920 --> 00:54:28,840
+طب دخليها لأكتر شوية جربى اصحبي طب ارفعي الزاوية
+
+734
+00:54:28,840 --> 00:54:36,640
+طب قليليها فكي الternicate و هكذاطيب في blood stop
+
+735
+00:54:36,640 --> 00:54:44,020
+flowing إذا الدم توقف تماما أثناء يعني ال seriano
+
+736
+00:54:44,020 --> 00:54:47,220
+فمعناها .. يعني أثناء ال seriano في ال syringe
+
+737
+00:54:47,220 --> 00:54:50,920
+فمعناها ممكن يكون أحد الأسباب غير أنه أنا اتغير
+
+738
+00:54:50,920 --> 00:54:54,420
+الموضوع ممكن يكون صار فيه vein collapse فبدي أنا
+
+739
+00:54:54,420 --> 00:54:57,140
+أرفع ال tourniquet لإنه ممكن .. حكينا إنه ال
+
+740
+00:54:57,140 --> 00:55:01,240
+tourniquet إذا طولت ممكن تسببلي أيضا .. إيش ..
+
+741
+00:55:01,240 --> 00:55:07,620
+vein collapseفبالتالي .. فإني أرفع الternicate
+
+742
+00:55:07,620 --> 00:55:11,820
+يعني أنا رفعت الضغط عن الوريد فصار فيه سريان في ..
+
+743
+00:55:11,820 --> 00:55:17,340
+لل .. لل blood داخل الوريد فبالتالي ممكن يرجع يتكو
+
+744
+00:55:17,340 --> 00:55:21,400
+.. يطلع عند blood يرجع يصير ال flow مرة تانية يطلع
+
+745
+00:55:21,400 --> 00:55:27,660
+في السرنج، يعب في السرنج إذا ما نجحتش في إن أنا
+
+746
+00:55:27,660 --> 00:55:32,780
+أرجع سريان الدمداخل ال syringe ب .. برفع ال needle
+
+747
+00:55:32,780 --> 00:55:38,080
+بطلعها و بحط يعني بفك ال tourniquet و بطلع ال
+
+748
+00:55:38,080 --> 00:55:43,020
+needle و بحط قطنة و بخليه انه to apply pressure و
+
+749
+00:55:43,020 --> 00:55:47,380
+بشوف مكان تاني ان انا اسحب منه volume اخر من الدم
+
+750
+00:55:47,380 --> 00:55:50,980
+من اليد التانية او من الوريد اخر في نفس اليد the
+
+751
+00:55:50,980 --> 00:55:56,130
+needle may have pulled out of the veinاحيانا طبعا
+
+752
+00:55:56,130 --> 00:56:00,530
+يعني ان الدم حكينا ان اذا اتوقف اما كان صارله
+
+753
+00:56:00,530 --> 00:56:04,870
+collapse الوريد او انه ال needle اتحركت و ساعتها
+
+754
+00:56:04,870 --> 00:56:07,970
+ان انا بده اركز بده ارجع ال .. ال needle مكانها
+
+755
+00:56:07,970 --> 00:56:11,750
+مرة تانية اما ب .. بالحركة للامام او للخلف او اظبط
+
+756
+00:56:11,750 --> 00:56:17,690
+الزاوية او افك الترنكتتمام؟ هيك بنكون خلصنا
+
+757
+00:56:17,690 --> 00:56:22,230
+الجزئية المتعلقة بال van puncture أو الفليبتمي و
+
+758
+00:56:22,230 --> 00:56:25,230
+حكينا إن الفليبتمي هي نفسها ال van puncture يعني
+
+759
+00:56:25,230 --> 00:56:31,190
+الحصول على عينة دم من خلال إحداث خنخز أو تقب في
+
+760
+00:56:31,190 --> 00:56:34,850
+الوريد يعني إن أنا أحصل على عينة من الوريد تمام؟
+
+761
+00:56:34,850 --> 00:56:39,770
+عينة blood whole bloodطبعا مجرد ما حصلت على العينة
+
+762
+00:56:39,770 --> 00:56:43,630
+هذه بفضيها في الأنبوبة المناسبة سواء كانت أنبوبة
+
+763
+00:56:43,630 --> 00:56:46,950
+plain أو فيها edita أو فيها citrate أو heparin حسب
+
+764
+00:56:46,950 --> 00:56:51,790
+نوع الفحص طبعا كل فحص بده بيتطلب نوع معين من ال
+
+765
+00:56:51,790 --> 00:56:57,390
+tube طبعا و طبعا بعمل mix و بعمل labeling منيه و
+
+766
+00:56:57,390 --> 00:57:01,720
+بارسلها للمختبر لحتى نجري الفحص عليهاهك بنكون
+
+767
+00:57:01,720 --> 00:57:05,720
+خلصنا الجزئية اليوم محاضرتنا ال part one و ال part
+
+768
+00:57:05,720 --> 00:57:09,700
+two ل ال blood collection و اتعرفنا على انه انا
+
+769
+00:57:09,700 --> 00:57:12,440
+كيف اسحب عينة اما من ال skin سواء كان finger أو
+
+770
+00:57:12,440 --> 00:57:16,800
+heel او من الوريد في ال vent puncture و اتعلمنا
+
+771
+00:57:16,800 --> 00:57:22,600
+الأشياء النظرية المتعلقة بسحب العينة تمام؟
+
+772
+00:57:22,600 --> 00:57:27,830
+المتعلقة بسحب العينةبيضل الأشياء العملية أول إشي
+
+773
+00:57:27,830 --> 00:57:30,790
+راح أشرحلكم .. راح أورجيكم فيديو على ال YouTube
+
+774
+00:57:30,790 --> 00:57:35,650
+تمام؟ و أشرحلكم الخطوات يعني أرجع أذكركم
+
+775
+00:57:35,650 --> 00:57:41,650
+بالتعليمات اللي حكيناها و أيضا راح أصورلكم فيديو
+
+776
+00:57:41,650 --> 00:57:46,030
+اللي أنا .. أنا عم بسحب عين الدم و أورجيكم الأدوات
+
+777
+00:57:46,030 --> 00:57:50,970
+اللي أنا بستخدمها و مكوناتها بحيث أنه نستفكر و ن
+
+778
+00:57:50,970 --> 00:57:55,320
+.. ن .. نثبت المعلومات اللي ذكرناها خلالالمحاضرة
+
+779
+00:57:55,320 --> 00:57:57,820
+يعطيكم العافية موثقين بإذن الله
+
diff --git a/PL9fwy3NUQKwY9jl8xw-bWkVZkWcGGyspd/DG-2O71i3Fw.srt b/PL9fwy3NUQKwY9jl8xw-bWkVZkWcGGyspd/DG-2O71i3Fw.srt
new file mode 100644
index 0000000000000000000000000000000000000000..eb824bdfb9bafcf41cd69df85d068d8835b71837
--- /dev/null
+++ b/PL9fwy3NUQKwY9jl8xw-bWkVZkWcGGyspd/DG-2O71i3Fw.srt
@@ -0,0 +1,2366 @@
+1
+00:00:09,950 --> 00:00:13,210
+السلام عليكم ورحمة الله وبركاته طالبات العزيزات
+
+2
+00:00:13,210 --> 00:00:17,230
+اليوم إن شاء الله راح نشرح محاضرة بعنوان white
+
+3
+00:00:17,230 --> 00:00:21,530
+blood cell count. في نهاية هذه المحاضرة راح نكون
+
+4
+00:00:21,530 --> 00:00:26,370
+بنعرف كيف احنا بنعد خلايا الدم البيضاء بطريقة
+
+5
+00:00:26,370 --> 00:00:31,670
+manual، بطريقة يدوية، وكيف بنحصل على عدد خلايا الدم
+
+6
+00:00:31,670 --> 00:00:35,230
+البيضاء as a total، إذا white blood cell count
+
+7
+00:00:35,230 --> 00:00:40,330
+معناها عدد خلايا الدم البيضاء. في البداية خلينا نحكي
+
+8
+00:00:40,330 --> 00:00:44,090
+كتقديم بسيط عن الـ white blood cells و الـ
+
+9
+00:00:44,090 --> 00:00:48,110
+characteristics لها، خصائص هذه الخلايا. بيحكي لي
+
+10
+00:00:48,110 --> 00:00:53,530
+whenever a germ or infection enters the body, the
+
+11
+00:00:53,530 --> 00:00:57,450
+white blood cells have a variety of ways by which
+
+12
+00:00:57,450 --> 00:01:03,950
+they can attack. إذا كان في عندي germ، جراثيم أو
+
+13
+00:01:03,950 --> 00:01:09,850
+عدوى، مسبب لعدوى، أو حتى أجسام غريبة دخلت إلى جسم
+
+14
+00:01:09,850 --> 00:01:14,610
+الإنسان، تمام؟ فالـ white blood cells عندها variety
+
+15
+00:01:14,610 --> 00:01:19,690
+of ways، طرق متنوعة لتهاجم بها هذا الجسم الغريب
+
+16
+00:01:19,690 --> 00:01:24,230
+أو هذا الكائن الغريب اللي غزا جسم الإنسان أو دخل
+
+17
+00:01:24,230 --> 00:01:29,790
+إليه. Some will produce protective antibodies، بعض
+
+18
+00:01:29,790 --> 00:01:33,750
+هذه الخلايا، بعض أنواع من الـ white species ممكن
+
+19
+00:01:33,750 --> 00:01:39,750
+أنها تنتج antibodies، تمام؟ أجسام مضادة لحتى تحارب
+
+20
+00:01:39,750 --> 00:01:44,050
+فيها هذه الكائنات الغريبة أو الأجسام الغريبة that
+
+21
+00:01:44,050 --> 00:01:47,910
+will overpower the germ، تتغلب فيها من خلال الـ
+
+22
+00:01:47,910 --> 00:01:53,010
+antibodies، تتغلب على الكائنات الدقيقة أو
+
+23
+00:01:53,010 --> 00:01:57,350
+الميكروبات أو الأجسام الغريبة اللي غزت الجسم أو
+
+24
+00:01:57,350 --> 00:02:02,050
+دخلت إلى الجسم. Other will surround and devour the
+
+25
+00:02:02,050 --> 00:02:07,110
+bacteria. أنواع أخرى من هذه الخلايا ممكن أن تحيط
+
+26
+00:02:07,110 --> 00:02:13,890
+بالكائنات الدقيقة و تغزوها أو يعني تلتهمها أو
+
+27
+00:02:13,890 --> 00:02:20,130
+تقتلها، تمام؟ The white blood cells have a rather
+
+28
+00:02:20,130 --> 00:02:28,270
+short life cycle. بنظر إلى الـ RBCs، حكينا إنه فترة
+
+29
+00:02:28,270 --> 00:02:33,290
+حياتها 120 يوم، تمام. الـ white blood cells بالمقارنة
+
+30
+00:02:33,290 --> 00:02:37,610
+عندها short life cycle، حياتها أو فترة حياتها قصيرة
+
+31
+00:02:37,610 --> 00:02:42,050
+من few days إلى few weeks، من أيام قليلة إلى أسابيع
+
+32
+00:02:42,050 --> 00:02:47,770
+قليلة، تمام؟ إذا الـ RBC بتعيش مائة و عشرين يوم، الـ
+
+33
+00:02:47,770 --> 00:02:52,030
+white BC من few days إلى few weeks، البلاتلت بتعيش
+
+34
+00:02:52,030 --> 00:02:57,940
+عشر أيام تقريبًا. إذا كل خلية الها life span أو life
+
+35
+00:02:57,940 --> 00:03:03,000
+cycle، يعني فترة حياة هي بتعيش فيها. فالـ white
+
+36
+00:03:03,000 --> 00:03:06,080
+species بتعتبر فترة حياتها قصيرة، يعني لإنها
+
+37
+00:03:06,080 --> 00:03:12,760
+باستمرار بتقابل مثلاً خلايا متوفية، يعني dead cells،
+
+38
+00:03:12,760 --> 00:03:20,430
+أجسام غريبة، كائنات غريبة، بقايا، T-Shirt، يعني waste،
+
+39
+00:03:20,430 --> 00:03:24,330
+أحيانًا كل هاي الشغلات بتخلصنا منها داخل جسم
+
+40
+00:03:24,330 --> 00:03:30,410
+الإنسان، ف... لذلك هي فترة حياتها قصيرة، نوعًا ما.
+
+41
+00:03:30,410 --> 00:03:34,650
+Several different and diverse types of leukocytes
+
+42
+00:03:34,650 --> 00:03:40,530
+exist. احنا ذكرنا في محاضرة الـ blood components إنه
+
+43
+00:03:40,530 --> 00:03:46,490
+أنا في عندي أنواع من خلايا الدم البيضاء. حكينا إنه
+
+44
+00:03:46,490 --> 00:03:52,930
+في عندي خلايا بسميها محببة، تمام؟ granulocytes، ماذا
+
+45
+00:03:52,930 --> 00:03:57,610
+يعني محببة أو granulocytes؟ هي الخلايا اللي يوجد في
+
+46
+00:03:57,610 --> 00:04:02,270
+الـ cytoplasm تبعها حبيبات، تمام؟ هاي الحبيبات طبعًا
+
+47
+00:04:02,270 --> 00:04:06,830
+بيكون فيها إنزيمات هاضمة وإنزيمات قاتلة، والسلاح
+
+48
+00:04:06,830 --> 00:04:14,270
+تبعها هذا الـ store، مخزن السلاح لخلايا الدم المحببة،
+
+49
+00:04:14,270 --> 00:04:20,590
+تمام؟ في عندي خلايا غير محببة، تمام؟ وهاي الخلايا
+
+50
+00:04:20,590 --> 00:04:24,270
+غير المحببة اللي احنا بنسميها agranulocytes، و
+
+51
+00:04:24,270 --> 00:04:28,950
+حكينا إضافة الـ A في بداية الكلمة للنفي، تمام؟ A-
+
+52
+00:04:28,950 --> 00:04:33,330
+granulocytes يعني لا تحتوي على حبيبات، وحكينا إنها
+
+53
+00:04:33,330 --> 00:04:37,200
+هي الـ monocytes و الـ lymphocytes. هلأ رح نذكرهم و
+
+54
+00:04:37,200 --> 00:04:41,460
+نستعرضهم مع بعض مرة ثانية، ولكن خلينا بس نكمل.
+
+55
+00:04:41,460 --> 00:04:45,180
+بيحكي لي إنه هذه الخلايا، صح، أنا في عندي different
+
+56
+00:04:45,180 --> 00:04:49,260
+and diverse types of leukocytes، صح هي متنوعة و
+
+57
+00:04:49,260 --> 00:04:57,530
+عديدة، تمام؟ ولكن they are all، كلهم produced and
+
+58
+00:04:57,530 --> 00:05:03,110
+derived، تم إنتاجهم أو يعني مشتقين من multipotent
+
+59
+00:05:03,110 --> 00:05:06,890
+stem cells، من نفس الخلية الأم اللي بنسميها
+
+60
+00:05:06,890 --> 00:05:11,450
+hematopoietic stem cells، موجودة في الـ bone marrow. طيب نذكر
+
+61
+00:05:11,450 --> 00:05:15,890
+مع بعض أو نستعرض مع بعض هذه الصورة اللي بتوضح
+
+62
+00:05:15,890 --> 00:05:22,770
+خلايا الدم كلها بشكل كامل، وهي من وين جاية أو من
+
+63
+00:05:22,770 --> 00:05:26,870
+وين تم اشتقاقها. في عندي، احنا طبعًا احنا حكينا إنه
+
+64
+00:05:26,870 --> 00:05:30,830
+يتم تصنيع خلايا الدم في الـ bone marrow، في نخاع
+
+65
+00:05:30,830 --> 00:05:36,810
+العظم، بيكون في عندي hematopoietic stem cells. نجرب
+
+66
+00:05:36,810 --> 00:05:42,460
+نكبر الصورة. Hematopoietic Stem Cell اللي هي عبارة عن
+
+67
+00:05:42,460 --> 00:05:45,980
+multipotent stem cell، خلية جدعية ممكن تعطيني
+
+68
+00:05:45,980 --> 00:05:50,920
+عدد معين من الخلايا، يعني ممكن إنها تتميز إلى عدد
+
+69
+00:05:50,920 --> 00:05:57,820
+معين من الخلايا. فهي بصراحة لما بتنقسم تعطيني two
+
+70
+00:05:57,820 --> 00:06:04,080
+mother cells، خليتين أم، واحدة اسمها myeloid، myeloid
+
+71
+00:06:04,080 --> 00:06:08,120
+progenitor cells، myeloid، تمام؟ والخلية الأم
+
+72
+00:06:08,120 --> 00:06:13,140
+الثانية اسمها lymphoid. طيب الـ... الخلية الـ... الـ
+
+73
+00:06:13,140 --> 00:06:17,860
+myeloid، هاي لما بدها تنقسم وتتميز أيضًا في عندها
+
+74
+00:06:17,860 --> 00:06:22,280
+بعض خصائص التمايز، فهي بتنقسم وتتميز لمين؟ لـ
+
+75
+00:06:22,280 --> 00:06:28,280
+eosinophils، و basophils، و neutrophils، اللي هي الخلايا
+
+76
+00:06:28,280 --> 00:06:32,460
+الـ granulocytes، المحببة. ممكن تعطيني أيضًا monocytes،
+
+77
+00:06:32,460 --> 00:06:35,960
+وتعطيني erythrocytes اللي هي الـ RBCs، و platelets.
+
+78
+00:06:35,960 --> 00:06:38,980
+هدول كلهم مشتقين من نفس الخلية الأم اللي هي
+
+79
+00:06:38,980 --> 00:06:43,600
+الـ myeloid. طبعًا الـ lymphoid، إيش هتعطيني؟ الـ lymphoid
+
+80
+00:06:43,600 --> 00:06:48,620
+بتعطيني الـ T cells lymphocytes، و B cell lymphocytes،
+
+81
+00:06:48,620 --> 00:06:52,920
+و natural killer lymphocytes، و dendritic cells. يعني
+
+82
+00:06:52,920 --> 00:06:56,080
+في النهاية هي بتعطيني lymphocytes لحالها بأنواعها.
+
+83
+00:06:56,080 --> 00:06:59,860
+طبعًا lymphoid lymphocytes، myeloid بتعطيني الـ
+
+84
+00:06:59,860 --> 00:07:02,960
+granulocytes اللي هم ال eosinophils، basophils، و
+
+85
+00:07:02,960 --> 00:07:07,780
+neutrophils، بالإضافة لـ RBCs، و monocytes، و platelets.
+
+86
+00:07:07,780 --> 00:07:13,160
+طبعًا. طيب، بغض النظر، احنا اليوم عنوان محاضرتنا اللي
+
+87
+00:07:13,160 --> 00:07:16,100
+هو الـ white blood cell count، يعني أنا معنية في مين؟
+
+88
+00:07:16,100 --> 00:07:18,980
+معنية في الـ granulocytes اللي هم ال eosinophils، و
+
+89
+00:07:18,980 --> 00:07:23,600
+basophils، و neutrophils، ومعنية في الـ agranulocytes
+
+90
+00:07:23,600 --> 00:07:29,330
+اللي هي monocytes و lymphocytes، تمام؟ طيب حكينا إن
+
+91
+00:07:29,330 --> 00:07:34,630
+هم مشتقين من مين؟ من خلية واحدة بالأصل اللي هي الـ
+
+92
+00:07:34,630 --> 00:07:38,070
+hematopoietic stem cells، حكينا بنسميها multi-
+
+93
+00:07:38,070 --> 00:07:40,450
+potent stem cells. إيش يعني multipotent؟ يعني
+
+94
+00:07:40,450 --> 00:07:43,790
+خلايا... خلية جدعية يمكنها أن تتميز وتعطيني عدد
+
+95
+00:07:43,790 --> 00:07:54,070
+معين من الخلايا، تمام؟
+
+96
+00:07:58,070 --> 00:08:01,330
+Leukocytes are found throughout the body، موجودة في
+
+97
+00:08:01,330 --> 00:08:04,850
+جسم الإنسان، موجودة في الـ blood، في الـ lymphatic
+
+98
+00:08:04,850 --> 00:08:09,690
+system، في... موجودة أحيانًا في الـ tissues، يعني في
+
+99
+00:08:09,690 --> 00:08:13,970
+في الكبد، خلايا lymph... white species خاصة فيه،
+
+100
+00:08:13,970 --> 00:08:18,250
+يعني to haggle، أي كائن ممكن يخترق الكبد، في في الـ
+
+101
+00:08:18,250 --> 00:08:22,730
+spleen، في في الـ intestine، يعني وين الأعضاء اللي
+
+102
+00:08:22,730 --> 00:08:26,990
+موجودة، ممكن أن تتعرض إلى infection، موجود فيها خلايا
+
+103
+00:08:26,990 --> 00:08:30,970
+متمركزة من الـ leukocytes، بالإضافة إلى إنه أيضًا كمان
+
+104
+00:08:30,970 --> 00:08:35,010
+الـ leukocytes،  الـ white blood cells، يعني ممكن تكون
+
+105
+00:08:35,010 --> 00:08:39,110
+بتوجد في الـ circulation، في الدم، في مسار الدم، في
+
+106
+00:08:39,110 --> 00:08:42,390
+الدورة الدموية، وأيضًا في الـ lymphatic system،
+
+107
+00:08:43,370 --> 00:08:48,550
+بالجهاز الليمفاوي. The name white blood cells، من وين
+
+108
+00:08:48,550 --> 00:08:51,690
+جا؟ ليش سميناها احنا white blood cells؟ هل فعلاً هي
+
+109
+00:08:51,690 --> 00:08:56,670
+اسمها أو لونها أبيض لحتى سميناها white؟ بيحكي لي
+
+110
+00:08:56,670 --> 00:09:02,530
+فعليًا تم اشتقاق الاسم هذا لأنه from the fact that
+
+111
+00:09:02,530 --> 00:09:05,930
+after centrifugation of a blood sample, the white
+
+112
+00:09:05,930 --> 00:09:09,700
+blood cells are found in the buffy coat. تم اشتقاق
+
+113
+00:09:09,700 --> 00:09:12,700
+اسم الـ white blood cells لإنه لما احنا بنعمل
+
+114
+00:09:12,700 --> 00:09:16,680
+centrifugation، نفس عينة الدم على الـ centrifuge، الـ
+
+115
+00:09:16,680 --> 00:09:19,240
+white blood cells بتكون موجودة في طبقة الـ buffy
+
+116
+00:09:19,240 --> 00:09:24,460
+coat اللي هي أصلًا لونها أبيض. فعليًا الـ white blood
+
+117
+00:09:24,460 --> 00:09:29,020
+cells هي خلايا colorless، مش ملونة، تمام؟ ولكن نظرًا
+
+118
+00:09:29,020 --> 00:09:33,240
+لأنه هي موجودة في منطقة الـ buffy coat اللي بتأخذ
+
+119
+00:09:33,240 --> 00:09:41,000
+اللون الأبيض، تمام؟ فبنسميها احنا white blood cells، و
+
+120
+00:09:41,000 --> 00:09:46,500
+... احنا هذا... هنا بيشرح لي إنه لما احنا بنفصل عينة
+
+121
+00:09:46,500 --> 00:09:50,300
+الدم على الـ centrifuge، بيكون في عندي الـ buffy coat،
+
+122
+00:09:50,300 --> 00:09:53,500
+اللي هو عبارة عن thin layer of the nucleated cells،
+
+123
+00:09:53,500 --> 00:09:57,780
+تمام؟ موجود between the sedimented red blood cells
+
+124
+00:09:57,780 --> 00:10:01,400
+وبين البلازما، موجود بين الـ RBCs و البلازما، وهذا
+
+125
+00:10:01,400 --> 00:10:06,060
+ممكن يجيني تعريف في الأسئلة، ماذا... يعني إنه لما
+
+126
+00:10:06,060 --> 00:10:09,380
+أجي أحكيلك the thin layer of the nucleated cells
+
+127
+00:10:10,010 --> 00:10:14,370
+that is found between the RBCs layer and the plasma
+
+128
+00:10:14,370 --> 00:10:18,150
+layer، اللي بتحكي لي هي الـ buffy coat، وممكن أسألك
+
+129
+00:10:18,150 --> 00:10:20,610
+ماذا يحتوي الـ buffy coat؟ اللي بتحكي لي يحتوي على
+
+130
+00:10:20,610 --> 00:10:26,410
+white blood cells و platelets، طبعًا. طيب الـ... الـ white BCs
+
+131
+00:10:26,410 --> 00:10:30,410
+لها اسم ثاني اللي هي leukocytes. ماذا تعني
+
+132
+00:10:30,410 --> 00:10:34,870
+leukocytes؟ Leukocytes هي كلمة يونانية، تمام؟ و
+
+133
+00:10:34,870 --> 00:10:39,990
+معناها الـ white cells. Leuko white، معناها، و cyte
+
+134
+00:10:39,990 --> 00:10:44,650
+يعني cells. زي فكرة الـ RBCs، بنسميها erythrocytes، و
+
+135
+00:10:44,650 --> 00:10:48,430
+أيضًا هي كلمة يونانية، erythro معناها red، و الـ
+
+136
+00:10:48,430 --> 00:10:54,740
+cyte معناها cell، تمام؟ طيب مبدأ عد خلايا الدم
+
+137
+00:10:54,740 --> 00:10:58,320
+البيضاء، كيف احنا بنعد خلايا الدم البيضاء؟ في
+
+138
+00:10:58,320 --> 00:11:03,040
+البداية في عندي free-flowing capillary، أو ممكن
+
+139
+00:11:03,040 --> 00:11:06,040
+استخدم well-mixed anticoagulated venous blood.
+
+140
+00:11:08,000 --> 00:11:11,740
+بستخدم، يعني بأخذ حجم معين من الدم، ويكون في عندي
+
+141
+00:11:11,740 --> 00:11:15,640
+محلول EDTA، أنا بخلطه مع هذا الدم بحيث
+
+142
+00:11:15,640 --> 00:11:21,040
+إنه هذا المحلول يخلصني من الـ RBCs ويحفظ لي الـ WBCs
+
+143
+00:11:21,040 --> 00:11:25,820
+عشان أنا أتمكن من عدها. باستخدام شريحة معينة مخصصة
+
+144
+00:11:25,820 --> 00:11:30,720
+للعد، أنا بحط السائل أو المحلول اللي أنا حضرته، اللي
+
+145
+00:11:30,720 --> 00:11:34,700
+هو عبارة عن الدم المخفف، بحطه على هذه الشريحة،
+
+146
+00:11:34,700 --> 00:11:39,760
+وببدأ أعد على الـ microscope، تمام؟ هذه هي الفكرة
+
+147
+00:11:39,760 --> 00:11:43,560
+ببساطة. خلينا نتعمق فيها أكثر ونشوف إيش تفاصيلها
+
+148
+00:11:43,560 --> 00:11:47,890
+العلمية. Free-flowing capillary or well-mixed
+
+149
+00:11:47,890 --> 00:11:51,730
+anticoagulated venous blood is added to a specific
+
+150
+00:11:51,730 --> 00:11:56,390
+volume of the diluent in the micropipette. طبعًا احنا
+
+151
+00:11:56,390 --> 00:12:00,970
+ممكن أنا أستخدم عينة من الوريد، تمام؟ وأحطها في
+
+152
+00:12:00,970 --> 00:12:05,110
+tube، أنبوبة يعني عليها anticoagulant، تمام؟ وتصير هذه
+
+153
+00:12:05,110 --> 00:12:08,930
+العينة اسمها well-mixed anticoagulated venous
+
+154
+00:12:08,930 --> 00:12:09,370
+blood.
+
+155
+00:12:14,850 --> 00:12:17,990
+في tube، أنبوبة، لإنه احنا لما بنسحب عينة دم في الـ
+
+156
+00:12:17,990 --> 00:12:22,370
+أنبوبة، أنا بحكي لكم اعملوا good mixing، تمام؟ بنعمل
+
+157
+00:12:22,370 --> 00:12:29,290
+good mixing، وبعد هيك طبعًا أنا حكينا إنه هذا الـ
+
+158
+00:12:29,290 --> 00:12:34,130
+blood ممكن أنا أستخدمه عشان أعمله dilution مع
+
+159
+00:12:34,130 --> 00:12:38,610
+diluent. طيب في نوع آخر أو مصدر آخر للعينة تبعتي
+
+160
+00:12:38,610 --> 00:12:42,590
+ممكن يكون هو عبارة عن free-flowing capillary، يعني
+
+161
+00:12:42,590 --> 00:12:46,490
+من الـ skin puncture، تمام؟ من الـ... من الإصبع، و
+
+162
+00:12:46,490 --> 00:12:49,930
+مباشرة أعبي capillary. طب الـ capillary هذه إيش
+
+163
+00:12:49,930 --> 00:12:54,670
+خصائصها؟ الخصائص... اسمها؟ هي Thoma pipette. خلينا
+
+164
+00:12:54,670 --> 00:12:58,710
+نستعرض مع بعض صورة لهاي الـ pipette اللي هو الـ... الـ
+
+165
+00:12:58,710 --> 00:13:03,510
+... الـ capillary اللي احنا بنستخدمها ونشوف إيش هي
+
+166
+00:13:03,510 --> 00:13:07,470
+خصائصها. هي مش capillary بمعنى capillary زي الـ
+
+167
+00:13:07,470 --> 00:13:11,750
+capillary tubes اللي أنتم شفتوهم، لكن هي من الداخل
+
+168
+00:13:11,750 --> 00:13:18,010
+تمام، مصممة على إنه المحلول يتم تعبئته فيها
+
+169
+00:13:18,010 --> 00:13:21,890
+بالخاصية الشعرية، يعني هي من الداخل رفيعة كثير ولو
+
+170
+00:13:21,890 --> 00:13:25,810
+إنها ممكن تكون سميكة من برا، تمام؟ لكن من جوا رفيعة
+
+171
+00:13:25,810 --> 00:13:31,010
+كثير، تمام؟ في عندي طبعًا واحدة بتكون مخصصة لعد الـ
+
+172
+00:13:31,010 --> 00:13:35,510
+RBCs، وواحدة مخصصة لعد الـ white blood cells. احنا اليوم راح
+
+173
+00:13:35,510 --> 00:13:39,950
+نذكر اللي احنا بصدد نتعرف عليها اللي هي الـ white
+
+174
+00:13:39,950 --> 00:13:44,070
+blood cells. Thoma pipette. لما نشرح الـ RBCs count راح نتطرق لـ
+
+175
+00:13:44,070 --> 00:13:49,740
+الـ RBCs pipette. طيب
+
+223
+00:17:46,560 --> 00:17:50,380
+reagents اللي ممكن أنا أستخدمها؟ طبعا إحنا حكينا
+
+224
+00:17:50,380 --> 00:17:54,000
+أن أنا لازم عينة الدم أخففها، بدي أستخدم diluting
+
+225
+00:17:54,000 --> 00:17:58,180
+fluid، طبعا الـ diluting fluid هذا في عندي أنواع منه
+
+226
+00:17:58,340 --> 00:18:01,560
+ممكن استخدم ascetic acid بتركيز 2% volume to
+
+227
+00:18:01,560 --> 00:18:07,020
+volume، وطبعا إحنا أخذنا محاضرة الـ dilution وشرحنا
+
+228
+00:18:07,020 --> 00:18:13,100
+كيف أنا بحضر تركيز volume to volume 2% مثلا أو 3%
+
+229
+00:18:13,100 --> 00:18:18,020
+حسب، وهذا ممكن تسأله عنه فبتراجعوا لمحاضرة الـ
+
+230
+00:18:18,020 --> 00:18:21,520
+dilution وبتعرفوا كيف إحنا بنحضر volume to volume
+
+231
+00:18:21,520 --> 00:18:27,440
+wet to wet أو wet to volume، تمام؟ طيب، diluting
+
+232
+00:18:27,440 --> 00:18:32,600
+fluid ممكن يكون Acetic acid 2% volume to volume أو
+
+233
+00:18:32,600 --> 00:18:37,760
+HCl 1% volume to volume أو حتى ممكن يكون عندي نوع
+
+234
+00:18:37,760 --> 00:18:41,400
+ثالث اللي هو الأكثر شيوعا واستخداما هو الـ Terg
+
+235
+00:18:41,400 --> 00:18:45,480
+solution وهو المحلول اللي إحنا راح نستخدمه، تمام؟
+
+236
+00:18:45,480 --> 00:18:50,100
+في المختبر، مكون من glacial acetic
+
+237
+00:18:50,100 --> 00:18:56,010
+acid، تمام؟ وcrystal violet صبغة اسمها crystal
+
+238
+00:18:56,010 --> 00:18:59,550
+violet، وdistilled water، هلأ الـ glacial acetic
+
+239
+00:18:59,550 --> 00:19:04,110
+acid حمض، تمام، وظيفته أنه يكسر لي الخلايا بمساعدة
+
+240
+00:19:04,110 --> 00:19:08,530
+أيضا الصبغة كمان بتساعد على أنها تخرب الـ membrane
+
+241
+00:19:08,530 --> 00:19:14,580
+للخلايا، فبسهولة تكسرهالـ... لـ crystal violet راح
+
+242
+00:19:14,580 --> 00:19:18,100
+تصبغ لي أنوية... بما أنها صبغة راح تصبغ أنوية الـ
+
+243
+00:19:18,100 --> 00:19:23,720
+white pieces، فإذن أنا بإمكاني أن أشوف الخلايا
+
+244
+00:19:23,720 --> 00:19:27,900
+... تمام ... أنويتها من الداخل أو الخلايا من الداخل
+
+245
+00:19:27,900 --> 00:19:31,480
+مخدة اللون الأزرق اللي هو نوع اللون لـ crystal
+
+246
+00:19:31,480 --> 00:19:36,940
+violet، فإذن بإمكاني يعني أعدها بسهولة ب... من خلال
+
+247
+00:19:36,940 --> 00:19:43,990
+أن أعرف أنها هي مخدة اللون، تمام؟ طيب، الأدوات
+
+248
+00:19:43,990 --> 00:19:49,690
+اللي بدي إياها، محتاجة diluting fluid وحكينا راح
+
+249
+00:19:49,690 --> 00:19:55,730
+نستخدم في المختبر Terg ومحتاجة Thoma white pipet
+
+250
+00:19:55,730 --> 00:20:01,830
+اللي هي المدرجة من نصف لواحد لحد أحد عشر، حكينا الـ RBC's
+
+251
+00:20:01,830 --> 00:20:05,570
+النوع بتاعي بتكون مدرجة من نصف، واحد، مية، واحد، والـ bulb
+
+252
+00:20:05,570 --> 00:20:08,650
+تبعها كبير، أكبر من الـ white pieces زي ما أنتم
+
+253
+00:20:08,650 --> 00:20:11,750
+شايفين، كنتوا في الصورة هنا، الـ... الـ... الـ white
+
+254
+00:20:11,750 --> 00:20:16,510
+pieces، ال... الحجم تبعها أكبر، تمام؟ فهي عشان هيك
+
+255
+00:20:16,510 --> 00:20:22,110
+نهاية التدريج تبعها لـ 101 و... لإن عددها أكبر بكون
+
+256
+00:20:22,110 --> 00:20:26,430
+محتاجة أنها تتخفف أكثر، عشان هيك ال... ال... التدريج
+
+257
+00:20:26,430 --> 00:20:32,160
+أكبر هنا، التفاصيل هاي رح ناخدها في الـ RBCs count
+
+258
+00:20:32,160 --> 00:20:37,280
+lecture، ولكن إحنا حاليا رح نحكي فقط عن الـ
+
+259
+00:20:37,280 --> 00:20:41,080
+thermopipet اللي هي خاصة بعد الـ white pieces، حكينا
+
+260
+00:20:41,080 --> 00:20:45,940
+تدريجها نصف، وواحد، ولحد 11، تمام، محتاجة هذه الـ
+
+261
+00:20:45,940 --> 00:20:49,700
+thermopipet، محتاجة الـ hematocytometer chamber اللي
+
+262
+00:20:49,700 --> 00:20:53,580
+هي الشريحة المخصصة للعد، محتاجة cover slip اللي هي
+
+263
+00:20:53,580 --> 00:20:56,340
+القطعة الزجاجية اللي أنا بحطها على الشريحة علشان
+
+264
+00:20:56,340 --> 00:21:01,440
+تغطي لي العينة، وبإمكاني بعد هيك أن أستخدم... يعني
+
+265
+00:21:01,440 --> 00:21:05,220
+أحطها على الـ microscope وأعد، بلزمني microscope،
+
+266
+00:21:05,220 --> 00:21:10,260
+بلزمني wipe شاش أو جوز أو قطن، بلزمني برضه كمان
+
+267
+00:21:10,260 --> 00:21:15,120
+قطع أو... قطن مغموسة بالكحول اللي هي alcohol pads
+
+268
+00:21:17,120 --> 00:21:19,960
+تمام، إذن إيش الأدوات اللي بتلزمني؟ حكينا
+
+269
+00:21:19,960 --> 00:21:24,540
+Thoma pipet، بيكون في عندي أيضا متصلة بـ rubber، هذا
+
+270
+00:21:24,540 --> 00:21:28,160
+الـ rubber بيكون يا بنات معقم ونظيف، ومتصل بقطعة
+
+271
+00:21:28,160 --> 00:21:33,480
+عشان إحنا أحيانا بنستخدم الفم في سحب المحلول، وأيضا
+
+272
+00:21:33,480 --> 00:21:37,660
+في عندي هنا hematocytometer chamber اللي هو عبارة
+
+273
+00:21:37,660 --> 00:21:43,120
+عن شريحة مخصصة للعد، شايفين أنه مش زي الشرائح
+
+274
+00:21:43,120 --> 00:21:46,620
+العادية، الزجاجية العادية البسيطة، هي شريحة مصممة
+
+275
+00:21:46,620 --> 00:21:51,300
+بطريقة معينة تمكنني من عد الخلايا وحساب العدد
+
+276
+00:21:51,300 --> 00:21:56,480
+بطريقة accurate، تمام؟ فإذن هذه هي الأدوات اللي
+
+277
+00:21:56,480 --> 00:22:00,800
+بتلزمني، طبعا اللي هي hematocytometer، الـ chamber بيكون
+
+278
+00:22:00,800 --> 00:22:05,720
+موجود في plastic container خاص فيه، عشان إحنا نحافظ
+
+279
+00:22:05,720 --> 00:22:09,220
+عليه ما ينكسر، لإنه بنرجع بنستخدمه، مش زي الشريحة
+
+280
+00:22:09,220 --> 00:22:13,640
+العادية، استخدمته، حطيت عليه مسحة دم مثلا، بعد ما
+
+281
+00:22:13,640 --> 00:22:18,230
+أخلص بكبها، لأ، في عندي هذا، يعني أنا بعقمه وبرجع
+
+282
+00:22:18,230 --> 00:22:23,230
+بستخدمه لأكثر من عينة، لإنه يعني هو غالي الثمن و
+
+283
+00:22:23,230 --> 00:22:28,090
+ما بنفع إحنا نتلفه، ولازم نحافظ عليه، فإذن هو
+
+284
+00:22:28,090 --> 00:22:31,560
+بيكون موجود في plastic container، الـ
+
+285
+00:22:31,560 --> 00:22:35,920
+hematocytometer، تفاصيل أكثر عن هذا الـ chamber، الـ
+
+286
+00:22:35,920 --> 00:22:39,100
+hematocytometer counting chamber is used for cell
+
+287
+00:22:39,100 --> 00:22:42,960
+counting، هو عبارة عن... إحنا بنسميه hematocytometer
+
+288
+00:22:42,960 --> 00:22:47,380
+chamber... chamber، ولكن هو عبارة عن شريحة مخصصة
+
+289
+00:22:47,380 --> 00:22:51,500
+لعد الخلايا مثل ما ذكرنا، it's constructed so that
+
+290
+00:22:51,500 --> 00:22:54,220
+the distance between the bottom of the cover slip
+
+291
+00:22:54,220 --> 00:22:57,840
+and the surface of the counting area is point one
+
+292
+00:22:57,840 --> 00:23:02,300
+millimeter، هو عبارة عن مصمم بطريقة معينة بحيث أن لو
+
+293
+00:23:02,300 --> 00:23:05,620
+حطيت عليه cover slip اللي هو الغطاء الزجاجي اللي
+
+294
+00:23:05,620 --> 00:23:09,500
+أنا بحط عليه، تمام، المسافة حتكون ما بين الـ cover
+
+295
+00:23:09,500 --> 00:23:13,860
+slip والـ chamber حتكون مسافة point واحد
+
+296
+00:23:13,860 --> 00:23:18,320
+millimeter، تمام؟ اللي هي يعني ال... ال... المنطقة
+
+297
+00:23:18,320 --> 00:23:21,860
+أو المسافة اللي أنا بعبي فيها العينة، ارتفاع العينة
+
+298
+00:23:21,860 --> 00:23:25,800
+حيكون point واحد millimeter، لإنها حتكون موجودة في
+
+299
+00:23:25,800 --> 00:23:29,880
+ال... المنطقة ما بين الـ chamber والـ cover slip، the
+
+300
+00:23:29,880 --> 00:23:33,300
+surface of that chamber contains two square ruled
+
+301
+00:23:33,300 --> 00:23:38,240
+areas separated by a H shape، خليني أشوف، زي ما
+
+302
+00:23:38,240 --> 00:23:42,880
+أنتم شايفين، هذا هو الـ chamber، تمام؟ زي عامل شكل
+
+303
+00:23:42,880 --> 00:23:47,580
+الـ H، وإحنا بنعد؟ في عندي هنا مربع وهنا مربع
+
+304
+00:23:47,580 --> 00:23:52,540
+وبيكونوا مسطرين ب... تسطيرة معينة، حورجيها لكم
+
+305
+00:23:52,540 --> 00:23:56,660
+بالعين المجردة، أول ما نرجع على المختبر، على شان
+
+306
+00:23:56,660 --> 00:24:01,960
+أساس أنكم تشوفوها منيح، طبعا لو أنا جيت حطيت cover
+
+307
+00:24:01,960 --> 00:24:06,400
+slip، قطعة زجاجية cover على هذا الـ chamber، طبعا أنا
+
+308
+00:24:06,400 --> 00:24:10,780
+بحط الـ cover slip بعدين بعبي العينة، طيب، فإذن لو أنا
+
+309
+00:24:10,780 --> 00:24:14,140
+حطيت الـ cover slip راح يكون في عندي مسافة فاصلة
+
+310
+00:24:14,140 --> 00:24:17,540
+هنا بين ال... بين الـ chamber والـ cover slip، هذه
+
+311
+00:24:17,540 --> 00:24:20,820
+المسافة الفاصلة، ارتفاعها واحد من عشرة millimeter
+
+312
+00:24:20,820 --> 00:24:25,960
+هنا بتكون العينة، أو يعني هنا أنا ب... ب... بعمل
+
+313
+00:24:25,960 --> 00:24:30,170
+تعبيّة على العينة، بعبي العينة في هذه المنطقة، طبعا
+
+314
+00:24:30,170 --> 00:24:33,510
+لازم أنا لما أعبي العينة، تكون العينة فقط على
+
+315
+00:24:33,510 --> 00:24:37,630
+المربع، يعني حوافها جاية على حواف المربع بالضبط،
+
+316
+00:24:37,630 --> 00:24:42,210
+ما بنفع أن العينة تكون خارج عن حواف المربع، زي هذا
+
+317
+00:24:42,210 --> 00:24:46,070
+الشكل، أو حتى فيها air bubble، فيها فقاعات، يعني
+
+318
+00:24:46,070 --> 00:24:51,350
+فراغات، لإن هذا هيأثر على الحسابات، طبعا، فهيكون في
+
+319
+00:24:51,350 --> 00:24:55,710
+عندي خطأ، error، إذن لازم أنا أعبيها بشكل أن ما يكون
+
+320
+00:24:55,710 --> 00:25:02,000
+فيه فراغات، إنها ما تطفح على الجوانب، لازم تكون فقط
+
+321
+00:25:02,000 --> 00:25:06,340
+على حواف المربع اللي مخصص للعد، هذا المربع وهذا
+
+322
+00:25:06,340 --> 00:25:12,860
+تمام، وتكون يعني حجمها مناسب وكميتها مناسبة، تمام،
+
+323
+00:25:12,860 --> 00:25:17,760
+وما تكون طبعا يعني إنها هيك عاملة زي التفاف، دائرة،
+
+324
+00:25:17,760 --> 00:25:20,960
+أنه يعني الحجم ناقص، لأ، لازم تكون well-fit، يعني
+
+325
+00:25:20,960 --> 00:25:25,720
+تماماً، مخدة شكل المربع اللي هي جالسة فيه، هنا...
+
+326
+00:25:25,720 --> 00:25:29,080
+هذا شكل إيه؟ أو يعني التعليمات اللي خاصة بالتعبئة،
+
+327
+00:25:29,080 --> 00:25:34,200
+طبعا إحنا بنعد... ممكن نعد في مربع واحد في واحد
+
+328
+00:25:34,200 --> 00:25:37,760
+منهم، وممكن أعد في المربعين، وفي نهاية القراءة
+
+329
+00:25:37,760 --> 00:25:41,140
+أقسمها على اثنين، آخذ الـ average لها، أقسمها على
+
+330
+00:25:41,140 --> 00:25:44,660
+اثنين، تمام؟ طيب،
+
+331
+00:25:47,820 --> 00:25:51,640
+إيش ال... التجربة طبعاً؟ أول حاجة، سواء كان أنا
+
+332
+00:25:51,640 --> 00:25:55,040
+بستخدم معي الـ blood من skin puncture، يعني من
+
+333
+00:25:55,040 --> 00:26:00,040
+الإصبع، أو من EDTA tube، يعني سحبت... سحبت من وريد
+
+334
+00:26:00,040 --> 00:26:03,640
+ووضعتها في ال... في EDTA tube، عملت mixing منيح
+
+335
+00:26:03,640 --> 00:26:08,730
+مع الـ EDTA، سواء كانت العينة skin puncture أو vein
+
+336
+00:26:08,730 --> 00:26:14,210
+puncture، أنا لازم أملأ ال... ال... ال... ال... الـ
+
+337
+00:26:14,210 --> 00:26:14,490
+... ال... ال... ال... ال... ال... ال... ال... ال...
+
+338
+00:26:14,490 --> 00:26:15,030
+ال... ال... ال... ال... ال... ال... ال... ال... الـ
+
+339
+00:26:15,030 --> 00:26:15,590
+... ال... ال... ال... ال... ال... ال... ال... ال...
+
+340
+00:26:15,590 --> 00:26:16,330
+ال... ال... ال... ال... ال... ال... ال... ال... الـ
+
+341
+00:26:16,330 --> 00:26:16,870
+ال... ال... ال... ال... ال... ال... ال... ال... الـ
+
+342
+00:26:16,870 --> 00:26:19,630
+... ال... ال... ال... ال... ال... ال... ال... ال...
+
+343
+00:26:19,630 --> 00:26:19,690
+ال... ال... ال... ال... ال... ال... ال... ال... الـ
+
+344
+00:26:19,690 --> 00:26:21,850
+... ال... ال... ال... ال... ال... ال... ال... ال...
+
+345
+00:26:21,850 --> 00:26:26,170
+ال... ال... ال... الـ capillary أو الـ Thoma pipet
+
+346
+00:26:26,170 --> 00:26:29,890
+بالـ blood، طبعا، إذا أنا حطيتها في tube، حتتبهدل من
+
+347
+00:26:29,890 --> 00:26:35,610
+برا، فلازم أنا أمسحها بـ wipe، تمام، عشان أنظفها، أشيل
+
+348
+00:26:35,610 --> 00:26:40,390
+الـ excess من الـ blood اللي موجودة على السطح، أو
+
+349
+00:26:40,390 --> 00:26:43,550
+عليها من برا، تمام، لإن هذا هيأثر على الـ dilution،
+
+350
+00:26:43,800 --> 00:26:47,460
+تمام؟ إذن، أول حاجة، أملأ إيه؟ ال... ال... الـ Thoma
+
+351
+00:26:47,460 --> 00:26:52,320
+pipet لعند علامة النصف بالـ blood، بعدين امسحي، wipe
+
+352
+00:26:52,320 --> 00:26:55,020
+the outside of the capillary، بنمسح الـ capillary
+
+353
+00:26:55,020 --> 00:26:57,920
+من برا، علشان الـ excess blood اللي موجودة عليها من
+
+354
+00:26:57,920 --> 00:27:02,640
+برا، ممكن يأثر على الـ dilution، تمام؟ ليش؟ لإن أنا
+
+355
+00:27:02,640 --> 00:27:05,960
+هاي الـ capillary، بعد ما أملأها بالـ blood، بدي أحطها
+
+356
+00:27:05,960 --> 00:27:10,820
+بشكل عمودي في tube، وهذا الـ tube موجود فيه diluent،
+
+357
+00:27:10,820 --> 00:27:16,080
+تمام؟ و... و أسحب بالـ diluent، لأ، ال... الـ
+
+358
+00:27:16,080 --> 00:27:20,080
+capillary بالـ diluent لعند العلامة 11، تمام؟ لعند
+
+359
+00:27:20,080 --> 00:27:26,410
+العلامة 11، طيب، إيش اللي راح يصير بالضبط؟ خلينا نرجع
+
+360
+00:27:26,410 --> 00:27:30,110
+لشكل الكابيلاري عشان نشرح عليها، أنا مملأتها لعند
+
+361
+00:27:30,110 --> 00:27:33,970
+العلامة هنا نصف، blood، مسحتها ونظفتها من برا، عشان
+
+362
+00:27:33,970 --> 00:27:37,030
+الـ excess blood ممكن يؤثر على الـ dilution، بعد هيك
+
+363
+00:27:37,030 --> 00:27:40,870
+بأخذ الكابيلاري هادي بشكل عمودي، بفوت في محلول، تمام،
+
+364
+00:27:40,870 --> 00:27:45,050
+المحلول هذا عبارة عن diluent، تمام، it's a solution،
+
+365
+00:27:45,050 --> 00:27:51,490
+حكينا ال... رح أسحب، أو يعني رح أ... ال... ال...
+
+366
+00:27:51,490 --> 00:27:56,450
+أكمل في... تعبئة التوما بايبِت، بدي أوصل لعند
+
+367
+00:27:56,450 --> 00:28:01,890
+العلامة 11، تمام؟ طيب، إيش اللي راح يصير؟ الـ blood
+
+368
+00:28:01,890 --> 00:28:06,330
+هنا لما يجي يعبى التارك، الـ blood هيطلع... كله
+
+369
+00:28:06,330 --> 00:28:10,910
+حيستقر وين؟ هيستقر في منطقة المستودع، يعني هنا الـ
+
+370
+00:28:10,910 --> 00:28:15,210
+blood حيكون موجود، تمام؟ وهنا حيصير فقط موجود
+
+371
+00:28:15,210 --> 00:28:20,370
+تارك، وهنا موجود أيضا بقايا الـ blood مع ال...
+
+372
+00:28:20,370 --> 00:28:27,320
+التارك، طيب، بعد ما أملأ لحد العلامة 11، بجيب قطنة و
+
+373
+00:28:27,320 --> 00:28:32,540
+بفضي محتويات الـ stem هنا، اللي هي إيش صارت موجود
+
+374
+00:28:32,540 --> 00:28:36,940
+فيها؟ موجود فيها فقط تارك، تمام؟ فقط موجود فيها
+
+375
+00:28:36,940 --> 00:28:40,420
+تارك solution اللي هو الـ diluent، لإن الدم خلاص
+
+376
+00:28:40,420 --> 00:28:48,330
+صعد إلى منطقة المستودع، طيب، إذن بعد ما أملأ الـ capillary
+
+377
+00:28:48,330 --> 00:28:57,410
+لحد العلامة 11، طبعا لازم أسكرها هيك بعد ما أطلعها
+
+378
+00:28:57,410 --> 00:29:01,170
+من الـ diluent، بسكرها بالسبابة، لإن أي فتحة فيها
+
+379
+00:29:01,170 --> 00:29:05,390
+ممكن يرجع يكب المحلول منها، طبعا لازم أعملها مغلقة،
+
+380
+00:29:05,390 --> 00:29:11,450
+وبعد هيك بروح على قطنة، على قطعة قطن، بفضي محتويات
+
+381
+00:29:11,450 --> 00:29:16,670
+الـ stem هي الواحدة هذه، طبعا يعني بفضي تقريبا ثلاث
+
+382
+00:29:16,670 --> 00:29:21,490
+قطرات أو أربع قطرات، اللي هم محتويات الـ stem، وبعد
+
+383
+00:29:21,490 --> 00:29:26,770
+هيك بروح بمسح الـ... الـ hematocytometer وبنظفه
+
+384
+00:29:26,770 --> 00:29:33,930
+بالكحول، بعد بقطعة قطنية أو قمع... يعني جوز شاش
+
+385
+00:29:33,930 --> 00:29:39,630
+مبلل بالكحول، بنظفه، بحط عليه cover slip و
+
+386
+00:29:39,630 --> 00:29:44,310
+باستخدام الـ pipette من عن الطرف هنا، من عند حواف، أو
+
+387
+00:29:44,310 --> 00:29:49,510
+حافة الـ cover slip، بسير أفرغ محلول بحيث أنه يملأ
+
+388
+00:29:49,510 --> 00:29:56,630
+المربع ويعبيه بشكل حكينا متناسق، أنه ما يطفح من عن
+
+389
+00:29:56,630 --> 00:29:59,930
+الجوانب، ما يكون excess فيه عندي، وما يكون
+
+445
+00:34:12,650 --> 00:34:15,470
+المضغوط بهذا الشكل، و في المربع الرابع لما يكون
+
+446
+00:34:15,470 --> 00:34:19,570
+بهذا الشكل تمام؟ فإذا أنتِ when كدك تعرفي حالكِ في
+
+447
+00:34:19,570 --> 00:34:23,230
+أي مربع، مثلاً كنتِ هنا في أي مربع في .. مثلاً كنتِ هنا
+
+448
+00:34:23,230 --> 00:34:27,210
+و شفتِ إنه مضغوط على الجانب من هنا و من .. من تحت
+
+449
+00:34:27,210 --> 00:34:30,510
+فمعناها بدكِ تتخيلي الرسمة كلها، و تعرفي حالكِ إنكِ أنتِ
+
+450
+00:34:30,510 --> 00:34:34,230
+في المربع الثاني لو مثلاً، لإنه طبعاً هو ما بعرض لك كل
+
+451
+00:34:34,230 --> 00:34:38,430
+الرسمة، هذه الـ field، الـ field بيكون مركز على الـ 16
+
+452
+00:34:38,430 --> 00:34:42,470
+مربع اللي هنا تمام؟ و جايب لك .. بس عرض لك جزء من
+
+453
+00:34:42,470 --> 00:34:46,490
+المضغوط اللي محيط في الـ 16 مربع اللي أنتِ بتقرئي
+
+454
+00:34:46,490 --> 00:34:54,130
+فيهم تمام؟ ف .. وين اتجاه المضغوط، بتتخيلي أنتِ في
+
+455
+00:34:54,130 --> 00:34:59,230
+مخيلتكِ كيف الرسمة أو الشكل بشكل مكبر، و بتعرفي حالكِ
+
+456
+00:34:59,230 --> 00:35:02,390
+أنتِ وين بالظبط، ف .. و بتعرفي حالكِ من وين .. وين
+
+457
+00:35:02,390 --> 00:35:05,330
+بدكِ تتنقلي، يعني أنتِ مثلاً في المربع الأول شفتِ
+
+458
+00:35:05,330 --> 00:35:08,970
+المضغوط هنا و هنا، معناها أنتِ في المربع الأول، وين
+
+459
+00:35:08,970 --> 00:35:12,310
+بدكِ تنتقلي؟ بدكِ تنتقلي للثاني، فمعناها مثلاً بدكِ
+
+460
+00:35:12,310 --> 00:35:17,130
+تتحركي بهذا الاتجاه في الـ field طبعاً، أو بدكِ تنزلي
+
+461
+00:35:17,130 --> 00:35:21,310
+هيك عشان تروحي على الثالث، فإذا بيساعدكِ وين ..
+
+462
+00:35:21,310 --> 00:35:26,970
+تحددي وين أنتِ موجودة، و كيف بدكِ تنتقلي لأي مربع، و
+
+463
+00:35:26,970 --> 00:35:32,610
+كيف الاتجاه تبع حركتكِ تمام؟ هذه التفاصيل كلها راح
+
+464
+00:35:32,610 --> 00:35:37,430
+نشتغلها خطوة بخطوة مع بعض، فيعني إذا في شيء مبهم، أو
+
+465
+00:35:37,430 --> 00:35:42,070
+شيء تساؤلات، كيف احنا بنعمل هاي الشغلة، حاولوا أن
+
+466
+00:35:42,070 --> 00:35:48,090
+يعني تكونوا relaxed، و ما .. يعني ما تستغربوا، أو
+
+467
+00:35:48,090 --> 00:35:51,550
+تخافوا، لإنه الشيء راح يكون على التطبيق إن شاء الله
+
+468
+00:35:52,450 --> 00:35:56,310
+ما بحكيش إن هو هيكون سهل جداً، ممكن يكون في صعوبة
+
+469
+00:35:56,310 --> 00:35:59,510
+ولكن يمكن تجاوزها، و راح تتعلموا، و تكون إن شاء الله
+
+470
+00:35:59,510 --> 00:36:05,830
+متمكنين، و شاطرين، طيب احنا بس في النهاية بدي أذكر
+
+471
+00:36:05,830 --> 00:36:08,550
+أنّي أنا لما بتحرك بهذا الاتجاه، يعني أنا بتحرك
+
+472
+00:36:08,550 --> 00:36:12,790
+لليسار في الشريحة، بكون بتحرك لليمين على الـ chamber
+
+473
+00:36:12,790 --> 00:36:16,030
+لإنّ الحركة بتكون معاكسة، و مقلوبة، أيضاً الصورة
+
+474
+00:36:16,030 --> 00:36:20,230
+بتكون معاكسة، و مقلوبة تمام، طيب إيش اللي أنا بدي
+
+475
+00:36:20,230 --> 00:36:24,330
+أعدّه في الـ 16 مربع هدول؟ بدي أعد النقاط السوداء هاي
+
+476
+00:36:24,330 --> 00:36:29,010
+طب كيف أنا أعرف هذه النقطة السوداء؟ خلية، أو دبرس، و
+
+477
+00:36:29,010 --> 00:36:36,030
+غبار، أو مثلاً أشياء موسّخة، الشريحة ممكن تكون يعني
+
+478
+00:36:36,030 --> 00:36:40,020
+شيء مش خلايا، كيف أنا بدي أعرفه؟ ذاكرين تركيب الـ
+
+479
+00:36:40,020 --> 00:36:43,300
+microscope، لما حكينا إنّ الـ course adjustment عشان
+
+480
+00:36:43,300 --> 00:36:46,020
+أجيب الـ field، عشان أقرب المسافة ما بين الـ stage و
+
+481
+00:36:46,020 --> 00:36:49,900
+العدسات، و الـ fine adjustment علشان أشوف التفاصيل
+
+482
+00:36:49,900 --> 00:36:54,660
+التفاصيل تمام، فأنا بلعب بالـ fine adjustment إذا
+
+483
+00:36:54,660 --> 00:36:58,540
+ظهرت لي في داخل الخلية، هذه في داخل النقطة السوداء
+
+484
+00:36:58,540 --> 00:37:02,320
+لون أزرق تمام، أو حتى أخضر، يعني هو ممكن يكون على
+
+485
+00:37:02,320 --> 00:37:05,600
+أخضرار، لأ بسبب إضاءة الـ microscope، بس هو فعلياً
+
+486
+00:37:05,600 --> 00:37:11,390
+الصبغة لونها أزرق تمام، فإذا ظهر عندي لون في الداخل
+
+487
+00:37:11,390 --> 00:37:14,610
+معناه عادي، خليها ما أخذة لون، مصبوغة بالصبغة اللي
+
+488
+00:37:14,610 --> 00:37:18,030
+موجودة في مكونات الـ Turk solution، في مكونات الـ
+
+489
+00:37:18,030 --> 00:37:24,580
+dilutant اللي أنا استخدمتها، طيب الحساب، أو الحسابات
+
+490
+00:37:24,580 --> 00:37:30,300
+اللي احنا بدنا نجريها، لحتى نجيب التراكيز، أو العدد
+
+491
+00:37:30,300 --> 00:37:32,820
+الخلايا، الـ white pieces count، الـ total white
+
+492
+00:37:32,820 --> 00:37:36,960
+pieces count، طبعاً أنا عندي عمق العينة، طبعتي اللي
+
+493
+00:37:36,960 --> 00:37:40,920
+هي المسافة اللي أنا عملت فيها، أو ملأت فيها العينة
+
+494
+00:37:40,920 --> 00:37:45,280
+كانت 1 من 10 ملي متر، اللي هي المسافة الفاصلة ما
+
+495
+00:37:45,280 --> 00:37:50,840
+بين الـ chamber و cover slip تمام؟ طيب الـ .. الـ
+
+496
+00:37:50,840 --> 00:37:54,500
+dilution اللي أنا بستخدمه، أنا بعمل dilution واحد
+
+497
+00:37:54,500 --> 00:37:59,900
+لعشرين، من أين أجا .. من أين أتى هذا الواحد لعشرين؟
+
+498
+00:37:59,900 --> 00:38:05,310
+خلينا نرجع للـ Thaumabiotic و نستذكر مع بعض احنا إيش
+
+499
+00:38:05,310 --> 00:38:10,830
+ذكرنا بخصوص الـ Tom and pie، أنا ملأت الدم لعند النص
+
+500
+00:38:10,830 --> 00:38:19,530
+تمام، و بعد هيك كملت بالـ daily one لعند 11، يعني
+
+501
+00:38:19,530 --> 00:38:26,590
+كمان في عندي، نرجع هنا نعمل
+
+502
+00:38:29,870 --> 00:38:35,010
+بنحط notes، كان في عندي blood، خدنا نكتب هنا بس الـ
+
+503
+00:38:35,010 --> 00:38:45,050
+blood إلى diluent تمام؟ كان في عندي الـ blood حطيت
+
+504
+00:38:45,050 --> 00:38:58,330
+أنا نص تمام؟ و الـ .. الـ diluent كان 11 تمام؟ طيب، في
+
+505
+00:38:58,330 --> 00:39:02,170
+عندي محتويات الـ stem، أنا حكيت، فضّيتها، اللي كانت
+
+506
+00:39:02,170 --> 00:39:05,510
+daily want لحاله، تمام؟ ذاكرين .. ذاكرين لما
+
+507
+00:39:05,510 --> 00:39:10,230
+حكيت لكم الـ blood بيطلع على المستودع، و الـ .. الـ stem
+
+508
+00:39:10,230 --> 00:39:15,230
+اللي هي من صفر لـ واحد من .. لعند الواحد، هذه بتصير
+
+509
+00:39:15,230 --> 00:39:20,590
+daily want كامل، أو daily want صافي، ترك لحاله تمام، و
+
+510
+00:39:20,590 --> 00:39:24,530
+أنا بفضّي محتويات هذا الـ stem على قطعة قطن، اللي هي
+
+511
+00:39:24,530 --> 00:39:28,230
+الثلاث أو أربع قطرات اللي أنا بتخلص منهم، فأصبح أنا
+
+512
+00:39:28,230 --> 00:39:31,850
+خلصت .. اتخلصت من محتويات الـ stem، اللي هي عبارة عن
+
+513
+00:39:31,850 --> 00:39:41,210
+واحد، يعني صار عندي الـ dilution نص إلى .. إلى
+
+514
+00:39:41,210 --> 00:39:46,270
+عشرة، اللي هي الواحد اللي خلصت منه
+
+515
+00:39:49,070 --> 00:39:53,110
+تمام؟ اتخلصت منه، الواحد، طب ليش أنا عملت أصلاً، عبيته
+
+516
+00:39:53,110 --> 00:39:57,810
+لعند الـ 11؟ لأ، لإني أنا معنية إنّه يعني بهذه الـ ..
+
+517
+00:39:57,810 --> 00:40:00,950
+الـ .. الكمية من الـ daily want، أنا بكون فوتت الدم
+
+518
+00:40:00,950 --> 00:40:06,160
+للمستودع، يعني أمنتّه موجود في المستودع تمام؟ طيب
+
+519
+00:40:06,160 --> 00:40:09,560
+فإذا، و بعد هيك بقايا الـ stem اللي هي فيها daily
+
+520
+00:40:09,560 --> 00:40:13,880
+one لحالي، اللي هي أصلاً كلها حجمها واحد، بتخلص منه
+
+521
+00:40:13,880 --> 00:40:18,420
+على قطعة قطن، فبيصير عندي في النهاية أنا أخذة نص لـ
+
+522
+00:40:18,420 --> 00:40:24,800
+11، في واحد اتخلصت منه، يعني نص لـ 10، يعني واحد تمام
+
+523
+00:40:24,800 --> 00:40:25,440
+إلى
+
+524
+00:40:31,120 --> 00:40:34,940
+عشرين تمام؟ الـ dilution طبيعي واحد إلى
+
+525
+00:40:34,940 --> 00:40:40,980
+عشرين، تمام، جديد بالذكر، أحكيلكم إنّ هذه الـ .. الـ
+
+526
+00:40:40,980 --> 00:40:47,140
+bead اللي هنا، بعد ما أنا أوصل الدم للمستودع، هذه الـ
+
+527
+00:40:47,140 --> 00:40:50,860
+bead، لو أنا عملت mixing، رجّيت الـ .. الـ by bit طبعاً
+
+528
+00:40:50,860 --> 00:40:55,140
+سَكَرْتها بـ .. بأصابعي، سَكَرْت فوها من هنا و من هنا، و
+
+529
+00:40:55,140 --> 00:40:59,700
+عملت mixing تمام، هذه الـ beads راح تتحرك، و هي تخلط
+
+530
+00:40:59,700 --> 00:41:04,280
+الدم مع الـ diluent، و راح تساعد على تكسير خلايا الـ
+
+531
+00:41:04,280 --> 00:41:07,860
+RBCs، و تنصبغ الـ white BCs بالصبغة اللي موجودة في
+
+532
+00:41:07,860 --> 00:41:12,740
+المحلول، علشان في النهاية أنا أحصل على محلول متجانس
+
+533
+00:41:12,740 --> 00:41:19,890
+أملؤه داخل الـ chamber، طيب أنا عدّيت في مربعات، المربع
+
+534
+00:41:19,890 --> 00:41:24,990
+كل واحد منه كان ستة .. يعني مقسّم إلى ستة عشر مربع
+
+535
+00:41:24,990 --> 00:41:29,430
+صغير، هنا المربعات هذه، أنا عدّيت أربعة مربعات، كل واحد
+
+536
+00:41:29,430 --> 00:41:33,190
+فيهم ستة عشر مربع صغير، طب هذا المربع الكبير اللي فيه
+
+537
+00:41:33,190 --> 00:41:37,170
+ستة عشر، إيش أبعاده؟ عبارة عن واحد millimeter في واحد
+
+538
+00:41:37,170 --> 00:41:41,910
+millimeter أبعاده؟ تمام؟ يعني، طيب فإذا لو أنا بدي
+
+539
+00:41:41,910 --> 00:41:46,210
+أجي أخد حجم المربع الواحد، عبارة عن .. طب الحجم هو
+
+540
+00:41:46,210 --> 00:41:49,750
+عبارة عن إيش؟ ضرب ثلاثة أبعاد، طول في عرض في ارتفاع، طيب
+
+541
+00:41:49,750 --> 00:41:53,770
+واحد millimeter طول، و عرض واحد millimeter، طول، طب
+
+542
+00:41:53,770 --> 00:41:58,270
+ارتفاعه قديش كان؟ كان هو اللي هو المسافة، أو البعد
+
+543
+00:41:58,270 --> 00:42:04,210
+ما بين الـ chamber و الـ slide، الـ cover slip اللي
+
+544
+00:42:04,210 --> 00:42:11,620
+محطوط تمام؟ فإذا .. حجم المربع هيكون واحد من عشرة
+
+545
+00:42:11,620 --> 00:42:17,720
+ملي متر مكعب، و بما إنّ السوائل بتأخذ حجم الإناء اللي
+
+546
+00:42:17,720 --> 00:42:23,260
+بتوضع فيه، تمام؟ إذا حجم السائل اللي أنا قرأته في
+
+547
+00:42:23,260 --> 00:42:28,060
+المربع هذا، هو حجم المربع، الطول في العرض في
+
+548
+00:42:28,060 --> 00:42:31,540
+الارتفاع، في كم مسافة، كم ما بين الـ chamber هذا و
+
+549
+00:42:31,540 --> 00:42:37,960
+الـ cover slip تمام؟ طيب، أنا عدّيت في أربعة مربعات،
+
+550
+00:42:37,960 --> 00:42:41,000
+يعني خلينا نحسب إنّه أنا عدّيت في منطقة واحدة من
+
+551
+00:42:41,000 --> 00:42:45,100
+الـ chamber، في أربعة مربعات، هيّهم، تمام؟ المنطقة
+
+552
+00:42:45,100 --> 00:42:51,520
+الواحدة من الـ chamber بتكون هيك مسطّرة، تمام؟
+
+553
+00:42:51,520 --> 00:42:55,580
+هي الـ chamber، المنطقة الواحدة بتكون هيك مسطّرة، زي ما
+
+554
+00:42:55,580 --> 00:43:01,920
+شفنا، و هنا كمان موجود أيضاً مسطّرة أخرى، طيب أنا
+
+555
+00:43:01,920 --> 00:43:05,520
+عدّيت فيه أربعة مربعات، معناها الحجم اللي حسبته
+
+556
+00:43:05,520 --> 00:43:10,140
+للمربع الواحد، بضربه فيه أربعة، ففي عندي الحجم لأربعة
+
+557
+00:43:10,140 --> 00:43:13,660
+مربعات هو عبارة عن أربعة في الواحد من عشرة
+
+558
+00:43:13,660 --> 00:43:18,380
+millimeter مكعب، و هيعطيني 0.4 من عشرة، أو
+
+559
+00:43:18,380 --> 00:43:22,860
+point أربعة millimeter مكعب، الـ millimeter مكعب هو
+
+560
+00:43:22,860 --> 00:43:25,540
+نفس الـ microliter، طيب
+
+561
+00:43:31,840 --> 00:43:36,740
+نفترض إنّه أنا عدّيت في أربعة مربعات، خمسين خلية تمام؟
+
+562
+00:43:36,740 --> 00:43:39,540
+في الأربع مربعات كان مجموع العدد خمسين، نفترض
+
+563
+00:43:39,540 --> 00:43:46,020
+افتراض، طيب الخمسين كانوا موجودين في حجم 0.4 من
+
+564
+00:43:46,020 --> 00:43:51,560
+عشرة، يعني ملي متر مكعب، طب كم بيكون موجود
+
+565
+00:43:51,560 --> 00:43:57,330
+في الواحد ملي متر مكعب؟ لإنه في النهاية أنا، وحدة
+
+566
+00:43:57,330 --> 00:44:03,470
+قياس الخلايا اللي هي عبارة عن cells، أو اللي هي الوحدة
+
+567
+00:44:03,470 --> 00:44:10,030
+لعد الخلايا، cells per micrometer مكعب، تمام؟ per
+
+568
+00:44:10,030 --> 00:44:11,230
+micrometer مكعب
+
+569
+00:44:16,630 --> 00:44:20,150
+مش موجود القص هنا، مش عارفة من وين أجيبه، ما علينا
+
+570
+00:44:20,150 --> 00:44:24,050
+ملي متر مكعب، أو ممكن تكون اللي هي عبارة
+
+571
+00:44:24,050 --> 00:44:31,690
+عن cells per microliter، تمام، per microliter، طيب أنا
+
+572
+00:44:31,690 --> 00:44:34,930
+في الـ microliter الواحد، كم بيكون موجود في الـ
+
+573
+00:44:34,930 --> 00:44:38,350
+millimeter مكعب الواحد، كم بيكون موجود خلايا، هذا
+
+574
+00:44:38,350 --> 00:44:44,660
+اللي بيلزمني، ففي النهاية أنا بعمل زي ضرب تبادلي، و
+
+575
+00:44:44,660 --> 00:44:49,460
+بطلع عندي الخمسين في واحد على أربعة من عشرة، طبعاً
+
+576
+00:44:49,460 --> 00:44:53,760
+الـ volume correction هو الواحد لأربعة من عشرة
+
+577
+00:44:53,760 --> 00:44:57,100
+فدايماً بضربه في، و في العدد الخلايا اللي بيطلع
+
+578
+00:44:57,100 --> 00:45:01,020
+معايا طبعاً، و هيك طبعاً بضربه كمان أيضاً في الـ
+
+579
+00:45:01,020 --> 00:45:06,800
+dilution factor، في الـ dilution factor طبعاً، و
+
+580
+00:45:06,800 --> 00:45:18,360
+بالنهاية بحصل على الـ total طبعاً، of White PCs تمام؟
+
+581
+00:45:18,360 --> 00:45:22,880
+إذا في النهاية أنا بيلزمني أكون عارفة أبعاد المربع
+
+582
+00:45:22,880 --> 00:45:28,020
+عشان أجيب الحجم، و .. و أجيب .. و الحجم بيلزمني
+
+583
+00:45:28,020 --> 00:45:33,400
+عشان أنا في النهاية بدي، بدل ما أحسب عدد الخلايا في
+
+584
+00:45:33,400 --> 00:45:38,360
+0.4 ملي متر مكعب، بدي أحسبه في واحد ملي متر
+
+585
+00:45:38,360 --> 00:45:42,900
+مكعب تمام؟ فبـ .. بـ .. بعمل يعني .. بضرب العدد في
+
+586
+00:45:42,900 --> 00:45:48,270
+مقلوب هذا الـ volume تمام؟ فهيك بكون جبت الـ volume
+
+587
+00:45:48,270 --> 00:45:52,290
+correction، طب، العينة مخففة، فعندي، فأنا بدي
+
+588
+00:45:52,290 --> 00:45:57,670
+العينة كـ .. كـ .. كـ .. الأصل، ما فيها عدد خلايا، فبدي
+
+589
+00:45:57,670 --> 00:46:00,830
+أضرب في الـ dilution factor، و احنا عرفنا دائماً
+
+590
+00:46:00,830 --> 00:46:04,430
+لما بدي أجيب الأصل، الـ original، بضرب في الـ dilution
+
+591
+00:46:04,430 --> 00:46:09,590
+factor، فإذا الـ total of white pieces count هيكون هو
+
+592
+00:46:09,590 --> 00:46:12,910
+عدد الخلايا اللي أنا عدّيتها في كم مربع؟ في أربعة
+
+593
+00:46:12,910 --> 00:46:17,090
+مربعات، عشان هيك لو أنا جبت يعني يا بعد في جهة
+
+594
+00:46:17,090 --> 00:46:20,790
+واحدة، يا إما بعد في الجهتين، و بقسم على اثنين، لإنه
+
+595
+00:46:20,790 --> 00:46:24,050
+لو عدّيت في الجهتين، هيكون في عندي ثمان قراءات، فأنا
+
+596
+00:46:24,050 --> 00:46:28,330
+بدي لأ، أنا بدي فقط أربعة قراءات، مجموع أربعة قراءات
+
+597
+00:46:28,330 --> 00:46:33,800
+تمام؟ مجموع الخلايا في أربعة مربعات فقط، لا غير، و
+
+598
+00:46:33,800 --> 00:46:36,560
+بضربه في الـ volume correction، و أيضاً في الـ
+
+599
+00:46:36,560 --> 00:46:40,120
+dilution factor، طبعاً هذه التفاصيل إن شاء الله ممكن
+
+600
+00:46:40,120 --> 00:46:48,380
+نوضحها أكثر في المعمل، و ننتقل إلى الـ discussion، أو
+
+601
+00:46:48,380 --> 00:46:53,500
+الجزئية اللي بتتعلق بصرْد .. صرد مصادر الـ error، و
+
+602
+00:46:53,500 --> 00:46:56,820
+كيف احنا نحلها، لو كان في عند الشخص عنده
+
+603
+00:46:56,820 --> 00:47:02,800
+leukocytosis، إيش يعني leukocytosis؟ يعني زيادة، high
+
+604
+00:47:03,520 --> 00:47:08,640
+أو elevated leukocyte count، عدد كبير، أو ارتفاع
+
+605
+00:47:08,640 --> 00:47:13,060
+كبير في عدد خلايا الدم البيضاء، معناها إيش بدي أعمل؟
+
+606
+00:47:13,060 --> 00:47:16,220
+بدي أعمل dilution أكبر، بدل ما أعمل dilution واحد
+
+607
+00:47:16,220 --> 00:47:20,160
+لعشرين، بعمل واحد لماية، dilution خارجي في الـ tube، مش
+
+608
+00:47:20,160 --> 00:47:22,860
+شرط أستخدم الـ Thaumapipet، لأنها مش هتوفر لي هذا الـ
+
+609
+00:4
+
+667
+00:51:45,020 --> 00:51:50,260
+عنده leukocytopenia ما عملت dilution كويس في حالات
+
+668
+00:51:50,260 --> 00:51:54,720
+الـ leukocytosis ما أخدتش الحجم الدم اللازم فإذا أنا
+
+669
+00:51:54,720 --> 00:52:01,760
+ما أخدتش كمية دم معبرة تمام، فبالتالي الفحص التابعي
+
+670
+00:52:01,760 --> 00:52:04,940
+أيضا مش راح يكون معبر، والنتائج انتباهتي مش راح
+
+671
+00:52:04,940 --> 00:52:08,610
+تكون دقيقة. failure to mix well إذا أنا ما عملت
+
+672
+00:52:08,610 --> 00:52:14,070
+mixing منيح للـ blood مع الـ .. الـ diluent. معناها
+
+673
+00:52:14,070 --> 00:52:18,210
+أنا برضه كمان هنا ممكن آخد concentrated أو آخد
+
+674
+00:52:18,210 --> 00:52:24,430
+مثلا دم مش مكسرة فيه الـ RBCs، فبالتالي هتعيق عملية
+
+675
+00:52:24,430 --> 00:52:29,660
+القراءة. failure to discard the first four drops لو
+
+676
+00:52:29,660 --> 00:52:33,560
+أنا ما اتخلصت من الأربع قطرات أو الثلاث قطرات اللي
+
+677
+00:52:33,560 --> 00:52:40,380
+بيكونوا موجودين في أول الجزء من الـ chamber.  بعيبة اللي
+
+678
+00:52:40,380 --> 00:52:45,060
+هو الـ stem. معناها أنه أنا لما هأجي أملأ الـ chamber
+
+679
+00:52:45,060 --> 00:52:50,000
+هاملاه فيهم هدول القطرات، يعني هاملأه بـ تركيز
+
+680
+00:52:50,000 --> 00:52:53,320
+solution لحال، مش راح يكون في عندي عينة كافية على
+
+681
+00:52:53,320 --> 00:52:56,140
+الـ chamber، فبالتالي هيكون الـ white blood cells count
+
+682
+00:52:56,140 --> 00:53:01,430
+قليل نتيجة أن أنا ما حطيتش عينة كفاية. failure to .. و
+
+683
+00:53:01,430 --> 00:53:04,850
+أيضا كمان الـ dilution تبعي هيكون فيه خلال. failure
+
+684
+00:53:04,850 --> 00:53:07,710
+to barely charge the counting chamber لو أنا
+
+685
+00:53:07,710 --> 00:53:11,910
+ما مملقتش الـ chamber بطريقة صحيحة، أيضا كمان راح يكون
+
+686
+00:53:11,910 --> 00:53:15,730
+في عندي خلال، لإن زي ما أنتم شايفين الحسابات تبعتي
+
+687
+00:53:15,730 --> 00:53:19,930
+بلزمني الحجم اللي كان .. الحجم الـ blood اللي هو
+
+688
+00:53:19,930 --> 00:53:25,790
+حجم المربعات اللي كان يملأها الـ blood، تمام؟ و
+
+689
+00:53:25,790 --> 00:53:30,070
+علشان أحصل على volume correction، وبلزمني أيضًا
+
+690
+00:53:30,070 --> 00:53:33,510
+dilution factor عشان أحصل على اللي .. اللي هو
+
+691
+00:53:33,510 --> 00:53:39,240
+الصيغة كاملة. failure to properly charge the
+
+692
+00:53:39,240 --> 00:53:41,960
+counting chamber إذا أنا ما مملقتش الـ chamber بشكل
+
+693
+00:53:41,960 --> 00:53:46,060
+منيح، معناها القانون اللي أنا بأطبقه خطأ و مش معبر
+
+694
+00:53:46,060 --> 00:53:50,380
+فبالتالي نتائجي فيها خطأ. أيضا كمان في عندي مشاكل
+
+695
+00:53:50,380 --> 00:53:55,060
+تانية لـ الـ error أقل شيوعا، ولكن ممكن تكون
+
+696
+00:53:55,060 --> 00:53:58,760
+موجودة. الـ pipette بتكون فيها مشكلة مثلا، أو الـ chamber
+
+697
+00:53:58,760 --> 00:54:04,070
+فيه مشكلة، يكون inaccurate، تستيرته خطأ، أبعاده،
+
+698
+00:54:04,070 --> 00:54:09,070
+الـ pipette فيها مشكلة، انكسرات، سدادات أو إشي، moist
+
+699
+00:54:09,070 --> 00:54:12,850
+or unclean. الـ pipette إذا كانت الـ .. الـ pipette من جوا فيها
+
+700
+00:54:12,850 --> 00:54:17,390
+مبللة أو فيها سائل أو مش نظيفة، أيضا كمان هذا ..
+
+701
+00:54:17,390 --> 00:54:22,070
+يعني إشي راح يشغل حيز، و بالتالي راح يأثر على الحجم
+
+702
+00:54:22,070 --> 00:54:25,470
+و يأثر على الـ dilution تبعي. excessive pressure in
+
+703
+00:54:25,470 --> 00:54:29,690
+finger لو أنا أخدت عينة من الـ .. الـ finger من الـ
+
+704
+00:54:29,690 --> 00:54:33,490
+skin puncture و عملت pressure، يعني ضغط جامد، فمعناها
+
+705
+00:54:33,490 --> 00:54:37,170
+أنا بكون عم بطلع عصارة خلايا شجوس، فبالتالي الـ
+
+706
+00:54:37,170 --> 00:54:40,470
+blood هيكون diluted، فبالتالي مش هيكون أيضا معبر.
+
+707
+00:54:41,390 --> 00:54:46,050
+هأحصل على reduced أو low white blood cells count too.
+
+708
+00:54:46,050 --> 00:54:48,930
+little or too much diluting fluid لو أنا حطيت
+
+709
+00:54:48,930 --> 00:54:52,870
+diluting fluid بكميات كبيرة أو بكميات قليلة، هذا
+
+710
+00:54:52,870 --> 00:54:56,930
+يأثر على الـ dilution. slowness in manipulation وهي
+
+711
+00:54:56,930 --> 00:55:04,190
+نقطة مهمة، أنه نسرع أو أنه نكون هيك يعني، كيف دي
+
+712
+00:55:04,190 --> 00:55:09,650
+أحكيها ناشطين، يعني أنه مش بطيء في الخطوات، و روح
+
+713
+00:55:09,650 --> 00:55:13,470
+نفارطة لأ، لإنه فعليا مثلا لو أنت بدك تاخدي عينة من
+
+714
+00:55:13,470 --> 00:55:16,510
+الـ skin puncture عشان تعبي فيها الـ chamber، لو أنت ما
+
+715
+00:55:16,510 --> 00:55:20,330
+أسرعتيش و حطيتيه و على طول ملأتي الـ blood للنص، وراه
+
+716
+00:55:20,330 --> 00:55:24,030
+على طول مباشرة حطيتِ الـ diluent، الدم رح يتجلط لإن
+
+717
+00:55:24,030 --> 00:55:27,570
+أنت عم تاخديه مباشرة من الـ skin puncture، يعني
+
+718
+00:55:27,570 --> 00:55:31,590
+ما فيش anticoagulant في الموضوع، فممكن يتجلط و يسدد
+
+719
+00:55:31,590 --> 00:55:35,600
+الـ chamber. لو كان في الـ .. في الـ chamber
+
+720
+00:55:35,600 --> 00:55:38,760
+air bubbles، برضه كمان معناها في مشكلة، فلذلك لازم
+
+721
+00:55:38,760 --> 00:55:43,440
+أنا أتحكم بنفسي منيح، و أنا عم باخد الـ volumes، و
+
+722
+00:55:43,440 --> 00:55:49,580
+أيضا كمان لما أفوت الـ pipette مثلا في solution
+
+723
+00:55:49,580 --> 00:55:53,380
+أفوتها بشكل عمودي، يعني في tricks  هتريح تتعلموها
+
+724
+00:55:53,380 --> 00:55:59,100
+بالشغل العملي، كيف أنت تمنعي حدوث الـ air bubbles أو
+
+725
+00:55:59,100 --> 00:56:02,750
+يعني ظهور الـ air bubbles في الشغل. برضه كمان الـ
+
+726
+00:56:02,750 --> 00:56:05,590
+air bubble في الـ counting chamber هتأثر على الحجم
+
+727
+00:56:05,590 --> 00:56:10,930
+اللي أنا عم بعده، فبالتالي ممكن تعطيني error إذا
+
+728
+00:56:10,930 --> 00:56:14,750
+كان الـ .. الـ .. الـ chamber مش نظيف مثلا، أو في
+
+729
+00:56:14,750 --> 00:56:20,110
+المحلول تبعي مش نظيف، مثلا في yeast أو fungi أو فطر
+
+730
+00:56:20,110 --> 00:56:23,900
+معين، عفن في المحلول، أو حتى كمان ممكن يكون على الـ
+
+731
+00:56:23,900 --> 00:56:28,520
+chamber، ما .. عليه غبار، أو ما مسح كويس، فبالتالي
+
+732
+00:56:28,520 --> 00:56:33,740
+هذا هيأثر، لإن هتعيق عملية العد، هذه الغبار و الـ ..
+
+733
+00:56:33,740 --> 00:56:39,840
+الـ contamination. Presence of many nucleated red
+
+734
+00:56:39,840 --> 00:56:43,280
+cells causing a high white cell count إذا كان في
+
+735
+00:56:43,280 --> 00:56:48,580
+عندي العديد من الخلايا الـ .. الـ red blood cells nucleated
+
+736
+00:56:48,580 --> 00:56:52,920
+تمام، معناها هذا هيعطيني error، فلذلك، أو إذا كان أنا
+
+737
+00:56:52,920 --> 00:56:57,360
+بشتغل white blood cells count مع blood film مع بعض، و لمحت
+
+738
+00:56:57,360 --> 00:57:01,820
+في الـ blood film nucleated red blood cells، على طول لازم
+
+739
+00:57:01,820 --> 00:57:05,700
+أروح أطبق قانون الـ corrected white blood cells count
+
+740
+00:57:07,130 --> 00:57:10,110
+mistakes in counting or calculation إذا كان أنا
+
+741
+00:57:10,110 --> 00:57:14,950
+طبقت القانون غلط، مثلا يعني هنا حطيت الـ  N  تكون
+
+742
+00:57:14,950 --> 00:57:20,010
+اللي هي مجموع عدد الخلايا في الـ 8 مربعات مثلا، أو
+
+743
+00:57:20,010 --> 00:57:23,710
+في 3 مربعات غلط، إذا أنا عديت في 8، يعني عديت في
+
+744
+00:57:23,710 --> 00:57:30,730
+الجهتين فوق و تحت، لازم أقسم على 2، لازم هذا يكون الـ
+
+745
+00:57:30,730 --> 00:57:33,870
+number of cells، هو مجموع الخلايا في الأربع مربعات
+
+746
+00:57:33,870 --> 00:57:34,730
+تمام؟
+
+747
+00:57:39,680 --> 00:57:43,900
+أو حتى في الحسابات، يعني مثلا ما جبت الـ correction
+
+748
+00:57:43,900 --> 00:57:50,920
+volume أو الـ volume correction صح، ما جبت الـ
+
+749
+00:57:50,920 --> 00:57:57,000
+dilution factor بشكل صح، دي برضه كمان رح يأثر، بهيك
+
+750
+00:57:57,000 --> 00:58:00,460
+بنكون وصلنا لنهاية محاضرتنا لليوم، أتمنى أنها تكون
+
+751
+00:58:00,460 --> 00:58:04,180
+واضحة و مش صعبة عليكم، يعطيكم ألف عافية
diff --git a/PL9fwy3NUQKwY9jl8xw-bWkVZkWcGGyspd/DG-2O71i3Fw_postprocess.srt b/PL9fwy3NUQKwY9jl8xw-bWkVZkWcGGyspd/DG-2O71i3Fw_postprocess.srt
new file mode 100644
index 0000000000000000000000000000000000000000..a854e0fd7d96724f3574eb0a87fa2fe0918d02f9
--- /dev/null
+++ b/PL9fwy3NUQKwY9jl8xw-bWkVZkWcGGyspd/DG-2O71i3Fw_postprocess.srt
@@ -0,0 +1,3004 @@
+1
+00:00:09,950 --> 00:00:13,210
+السلام عليكم ورحمة الله وبركاته طالبات العزيزات
+
+2
+00:00:13,210 --> 00:00:17,230
+اليوم ان شاء الله راح نشرح محاضرة بعنوان white
+
+3
+00:00:17,230 --> 00:00:21,530
+blood cell count في نهاية هذه المحاضرة راح نكون
+
+4
+00:00:21,530 --> 00:00:26,370
+بنعرف كيف احنا بنعد خلايا الدم البيضاء بطريقة
+
+5
+00:00:26,370 --> 00:00:31,670
+manual بطريقة يدوية وكيف بنحصل على عدد خلايا الدم
+
+6
+00:00:31,670 --> 00:00:35,230
+البيضاء as a total اذا white blood cell count
+
+7
+00:00:35,230 --> 00:00:40,330
+معناها عد خلايا الدم البيضاءفي البداية خلونا نحكي
+
+8
+00:00:40,330 --> 00:00:44,090
+كتقديم بسيط عن ال white blood cells و ال
+
+9
+00:00:44,090 --> 00:00:48,110
+characteristic لإلها، خصائص هذه الخلايا بيحكيلي
+
+10
+00:00:48,110 --> 00:00:53,530
+whenever a germ or infection enter the body the
+
+11
+00:00:53,530 --> 00:00:57,450
+white blood cell have a variety of ways by which
+
+12
+00:00:57,450 --> 00:01:03,950
+they can attack إذا كان في عندي germ، جراثيم أو
+
+13
+00:01:03,950 --> 00:01:09,850
+عدوى مسبب لعدوى أو حتى أجسام غريبةدخلت إلى جسم
+
+14
+00:01:09,850 --> 00:01:14,610
+الإنسان، تمام؟ فال white blood cells عندها variety
+
+15
+00:01:14,610 --> 00:01:19,690
+of ways، أطرق متنوعة لتهاجم بها هذا الجسم الغريب
+
+16
+00:01:19,690 --> 00:01:24,230
+أو هذا الكائن الغريب اللي غزى جسم الإنسان أو دخل
+
+17
+00:01:24,230 --> 00:01:29,790
+إليهSome will produce protective antibodies، بعض
+
+18
+00:01:29,790 --> 00:01:33,750
+هذه الخلايا، بعض أنواع من ال white species ممكن
+
+19
+00:01:33,750 --> 00:01:39,750
+إنها تنتج antibodies تمام؟ أجسام مضادة لحتى تحارث
+
+20
+00:01:39,750 --> 00:01:44,050
+فيها هذه الكائنات الغريبة أو الأجسام الغريبةthat
+
+21
+00:01:44,050 --> 00:01:47,910
+will overpower the germ تتغلب فيها من خلال ال
+
+22
+00:01:47,910 --> 00:01:53,010
+antibodies تتغلب على الكائنات الدقيقة أو
+
+23
+00:01:53,010 --> 00:01:57,350
+الميكروبات أو الأجسام الغريبة اللي غزت الجسم أو
+
+24
+00:01:57,350 --> 00:02:02,050
+دخلت إلى الجسمother will surround and devour the
+
+25
+00:02:02,050 --> 00:02:07,110
+bacteria أنواع أخرى من هذه الخلايا ممكن أن تحيط
+
+26
+00:02:07,110 --> 00:02:13,890
+بالكائنات الدقيقة و تغزوها أو يعني تلتهمها أو
+
+27
+00:02:13,890 --> 00:02:20,130
+تقتلها تمام؟ the white blood cellhave a rather
+
+28
+00:02:20,130 --> 00:02:28,270
+short life cycle بنظر إلى ال RBCs حكينا إنه فترة
+
+29
+00:02:28,270 --> 00:02:33,290
+حياتها 120 يوم تمام ال white blood cell بالمقارنة
+
+30
+00:02:33,290 --> 00:02:37,610
+عندها short life cycleحياتها أو فترة حياتها قصيرة
+
+31
+00:02:37,610 --> 00:02:42,050
+من few days ل few weeks من أيام قليلة إلى أسابيع
+
+32
+00:02:42,050 --> 00:02:47,770
+قليلة، تمام؟ إذا ال RBC بتعيش مائة و عشرين يوم ال
+
+33
+00:02:47,770 --> 00:02:52,030
+white BC من few days ل few weeks البلاتلت بتعيش
+
+34
+00:02:52,030 --> 00:02:57,940
+عشر أيام تقريبافإذا كل خلية إلها life span أو life
+
+35
+00:02:57,940 --> 00:03:03,000
+cycle يعني فترة حياة هي بتعيش فيها فال white
+
+36
+00:03:03,000 --> 00:03:06,080
+species بتعتبر فترة حياتها قصيرة يعني لإنها
+
+37
+00:03:06,080 --> 00:03:12,760
+باستمرار بتقابل مثلا خلايا متوفة يعني dead cells
+
+38
+00:03:12,760 --> 00:03:20,430
+أجسام غريبة كائنات غريبة بقايا T-Shirtيعني waste
+
+39
+00:03:20,430 --> 00:03:24,330
+أحيانا كل هاي الشغلات بتكون بتخلصنا منها داخل جسم
+
+40
+00:03:24,330 --> 00:03:30,410
+الإنسان ف .. لذلك هي فترة حياتها قصيرة نوع عامة
+
+41
+00:03:30,410 --> 00:03:34,650
+several different and diverse types of leukocyte
+
+42
+00:03:34,650 --> 00:03:40,530
+exist احنا ذكرنا في محاضرة ال blood component أنه
+
+43
+00:03:40,530 --> 00:03:46,490
+أنا في عندي أنواع من خلايا الدم البيضاءحكينا إنه
+
+44
+00:03:46,490 --> 00:03:52,930
+في عندي خلايا بسميها محببة تمام granulocyte وماذا
+
+45
+00:03:52,930 --> 00:03:57,610
+يعني محببة أو granulocyte؟ هي الخلايا اللي يوجد في
+
+46
+00:03:57,610 --> 00:04:02,270
+الcytoplasm تبعها حبيبات تمام؟ هاي الحبيبات طبعا
+
+47
+00:04:02,270 --> 00:04:06,830
+بيكون فيها انزيمات هاضمة وانزيمات قاتلة و السلاح
+
+48
+00:04:06,830 --> 00:04:14,270
+تبعها هذا ال store مخزن السلاح لخلايا الدم المحببة
+
+49
+00:04:14,270 --> 00:04:20,590
+تمام؟في عندي خلايا غير محببة، تمام؟ و هاي الخلايا
+
+50
+00:04:20,590 --> 00:04:24,270
+غير المحببة اللي احنا بنسميها A-granulocyte و
+
+51
+00:04:24,270 --> 00:04:28,950
+حكينا إضافة ال A في بداية الكلمة للنفي، تمام؟ A
+
+52
+00:04:28,950 --> 00:04:33,330
+-granulocyte يعني لا تحتوي على حبيبات و حكينا إنها
+
+53
+00:04:33,330 --> 00:04:37,200
+هي ال monocyte و ال lymphocyteهلأ رح نذكرهم و
+
+54
+00:04:37,200 --> 00:04:41,460
+نستعرضهم مع بعض مرة تانية ولكن خلونا بس نكمل
+
+55
+00:04:41,460 --> 00:04:45,180
+بحكيلي انه هذه الخلايا صح أنا في عندى different
+
+56
+00:04:45,180 --> 00:04:49,260
+and diverse types of leukocytes صح هي متنوعة و
+
+57
+00:04:49,260 --> 00:04:57,530
+عديدة تمام ولكن they are all كلهمproduced and
+
+58
+00:04:57,530 --> 00:05:03,110
+derived تم إنتاجهم أو يعني مشتقين من multi-potent
+
+59
+00:05:03,110 --> 00:05:06,890
+stem cell من نفس الخلية الأم اللي بنسميها
+
+60
+00:05:06,890 --> 00:05:11,450
+hematobiotic stem cell موجودة في البومارو طيب نذكر
+
+61
+00:05:11,450 --> 00:05:15,890
+مع بعض أو نستعرض مع بعض هذه الصورة اللي بتوضح
+
+62
+00:05:15,890 --> 00:05:22,770
+خلايا الدم كلها بشكل كامل وهي من وين جاية او من
+
+63
+00:05:22,770 --> 00:05:26,870
+وين تم اشتقاقهافي عندي احنا طبعا احنا حكينا انه
+
+64
+00:05:26,870 --> 00:05:30,830
+يتم تصنيع خلايا الدم في ال bone marrow في نخاع
+
+65
+00:05:30,830 --> 00:05:36,810
+العضم بيكون في عندي hematobiotic stem cell نجرب
+
+66
+00:05:36,810 --> 00:05:42,460
+نكبر الصورةHematobiotic Stem Cell اللي هي عبارة عن
+
+67
+00:05:42,460 --> 00:05:45,980
+Multipotential Stem Cell خلية جدعية ممكن تعطيني
+
+68
+00:05:45,980 --> 00:05:50,920
+عدد معين من الخلايا يعني ممكن إنها تتميز إلى عدد
+
+69
+00:05:50,920 --> 00:05:57,820
+معين من الخلايا فهي بصراحة لما بتنقسم تعطيني two
+
+70
+00:05:57,820 --> 00:06:04,080
+mother cells خليتين أم واحدة اسمها Myeloidmyeloid
+
+71
+00:06:04,080 --> 00:06:08,120
+progenitor cells myeloid تمام و الخلية الأم
+
+72
+00:06:08,120 --> 00:06:13,140
+التانية اسمها lymphoid طيب ال .. الخلية ال .. ال
+
+73
+00:06:13,140 --> 00:06:17,860
+myeloid هاي لما بدها تنقسم و تتميز ايضا في عندها
+
+74
+00:06:17,860 --> 00:06:22,280
+بعض خصائص الاستمثال فهي بتنقسم و تتميز لمين؟ ل
+
+75
+00:06:22,280 --> 00:06:28,280
+Xenophilو Bezophil و Neutrophil اللى هى الخلايا
+
+76
+00:06:28,280 --> 00:06:32,460
+الى granulocyte المحددة ممكن تعطينى ايضا monocyte
+
+77
+00:06:32,460 --> 00:06:35,960
+و تعطينى erythrocyte اللى هى ال RBCs و Blatulate
+
+78
+00:06:35,960 --> 00:06:38,980
+هدول كلهم مشتقين من نفس الخلية الأم اللى هى
+
+79
+00:06:38,980 --> 00:06:43,600
+الmyeloidطبعا ال lymphoid ايش هتعطيني؟ ال lymphoid
+
+80
+00:06:43,600 --> 00:06:48,620
+بتعطيني ال T cells lymphocyte و B cell lymphocyte
+
+81
+00:06:48,620 --> 00:06:52,920
+و natural killer lymphocyte و dendritic cell يعني
+
+82
+00:06:52,920 --> 00:06:56,080
+فى النهاية هى بتعطينى lymphocyte لحالها بأنواعها
+
+83
+00:06:56,080 --> 00:06:59,860
+طبعا lymphoid lymphocyte myeloid بتعطينى ال
+
+84
+00:06:59,860 --> 00:07:02,960
+granulocyte اللى هم ال xenophil, bezophil و
+
+85
+00:07:02,960 --> 00:07:07,780
+neutrophil بالاضافة ل RBCs و monocyte و black lead
+
+86
+00:07:07,780 --> 00:07:13,160
+طبعاطيب، بغض النظر احنا اليوم عنوان محاضرتنا اللي
+
+87
+00:07:13,160 --> 00:07:16,100
+هو ال white pieces count يعني أنا معنية في مين؟
+
+88
+00:07:16,100 --> 00:07:18,980
+معنية في ال granulocyte اللي هم ال xenophil و
+
+89
+00:07:18,980 --> 00:07:23,600
+bezophil و neutrophil و معنية في ال agranulocyte
+
+90
+00:07:23,600 --> 00:07:29,330
+اللي هي monocyte و lymphocyte، تمام؟طيب حكينا إن
+
+91
+00:07:29,330 --> 00:07:34,630
+هم مشتقين من مين؟ من خلية واحدة بالاصل اللي هي ال
+
+92
+00:07:34,630 --> 00:07:38,070
+hematobiotic stem cell حكينا بنسميها multi
+
+93
+00:07:38,070 --> 00:07:40,450
+potential stem cell إيش يعني multi potential؟ يعني
+
+94
+00:07:40,450 --> 00:07:43,790
+خلايا .. خلية جدعية يمكنها أن تتميز وتعطيني عدد
+
+95
+00:07:43,790 --> 00:07:54,070
+معين من الخلايا تمام؟
+
+96
+00:07:58,070 --> 00:08:01,330
+leukocytes are found throughout the body موجودة في
+
+97
+00:08:01,330 --> 00:08:04,850
+جسم الإنسان موجودة في ال blood في ال lymphatic
+
+98
+00:08:04,850 --> 00:08:09,690
+system في .. موجودة أحيانا في ال tissues يعني في
+
+99
+00:08:09,690 --> 00:08:13,970
+في الكبد خلايا lymph .. white species خاصة فيه
+
+100
+00:08:13,970 --> 00:08:18,250
+يعني to haggle أي كائن ممكن يخزق الكبد في في ال
+
+101
+00:08:18,250 --> 00:08:22,730
+spleen في في ال intestine يعني وين الأعضاء اللي
+
+102
+00:08:22,730 --> 00:08:26,990
+موجودة ممكن أن تتعرض إلى infectionموجود فيها خلايا
+
+103
+00:08:26,990 --> 00:08:30,970
+مثبتة من الـ leukocyte بالإضافة إلى إنه أيضًا كمان
+
+104
+00:08:30,970 --> 00:08:35,010
+الـ leukocyte ليه ال white disease يعني ممكن تكون
+
+105
+00:08:35,010 --> 00:08:39,110
+بتوجد بال circulation في الدم في مسار الدم في
+
+106
+00:08:39,110 --> 00:08:42,390
+الدورة الدموية و أيضًا في ال lymphatic system
+
+107
+00:08:43,370 --> 00:08:48,550
+بالجهاز اللينفاوي the name white blood cell من وين
+
+108
+00:08:48,550 --> 00:08:51,690
+اجا؟ ليش صميناها احنا white blood cell؟ هل فعلا هي
+
+109
+00:08:51,690 --> 00:08:56,670
+اسمها او لونها white لحتى صميناها white؟ بحكيلي
+
+110
+00:08:56,670 --> 00:09:02,530
+فعليا تم اشتقاق الاسم هذا لانه from the fact that
+
+111
+00:09:02,530 --> 00:09:05,930
+after centrifugation of a blood sample the white
+
+112
+00:09:05,930 --> 00:09:09,700
+blood cell are found in the puffy coatتم اشتقاق
+
+113
+00:09:09,700 --> 00:09:12,700
+اسم ال white blood cell لإنه لما احنا بنعمل
+
+114
+00:09:12,700 --> 00:09:16,680
+centrifugation نفس العين الدم على ال centrifuge ال
+
+115
+00:09:16,680 --> 00:09:19,240
+white blood cell بتكون موجودة في طبقة ال buffy
+
+116
+00:09:19,240 --> 00:09:24,460
+coat اللي هي أصلا لونها أبيض فعليا ال white blood
+
+117
+00:09:24,460 --> 00:09:29,020
+cell هي خلايا colorless مش ملونة تمام؟ ولكن نظرا
+
+118
+00:09:29,020 --> 00:09:33,240
+لإنه هي موجودة في منطقة ال buffy coat اللي تتاخذ
+
+119
+00:09:33,240 --> 00:09:41,000
+اللون الأبيض تمام؟فبنسميها احنا white blood cellو
+
+120
+00:09:41,000 --> 00:09:46,500
+.. احنا هذا .. هنا بيشرحلي انه لما احنا بنفصل عينة
+
+121
+00:09:46,500 --> 00:09:50,300
+الدم على ال centrifuge بيكون في عندي ال buffy coat
+
+122
+00:09:50,300 --> 00:09:53,500
+اللي هو عبارة عن thin layer of the nucleated cell
+
+123
+00:09:53,500 --> 00:09:57,780
+تمام؟ موجود between the sedimented red blood cell
+
+124
+00:09:57,780 --> 00:10:01,400
+وبتوين البلازمة موجود بين ال RBCs و البلازمة وهذا
+
+125
+00:10:01,400 --> 00:10:06,060
+ممكن يجيني تعريف في الأسئلة ماذا .. يعني انه لما
+
+126
+00:10:06,060 --> 00:10:09,380
+أجي أحكيلك the thin layer of the nucleated cell
+
+127
+00:10:10,010 --> 00:10:14,370
+that found between the RBCs layer and the plasma
+
+128
+00:10:14,370 --> 00:10:18,150
+layer اللي بتحكيلي هي ال buffet code وممكن أسألك
+
+129
+00:10:18,150 --> 00:10:20,610
+ماذا يحتوي ال buffet code اللي بتحكيلي يحتوي على
+
+130
+00:10:20,610 --> 00:10:26,410
+white pieces وplatelet طبعا طيبال .. ال white bc's
+
+131
+00:10:26,410 --> 00:10:30,410
+لها اسم تاني اللي هي lycosides ماذا تعني
+
+132
+00:10:30,410 --> 00:10:34,870
+lycosides؟ lycosides هي كلمة يونانية، تمام؟ و
+
+133
+00:10:34,870 --> 00:10:39,990
+معناها ال white cells lyco white معناها و site
+
+134
+00:10:39,990 --> 00:10:44,650
+يعني cells زي فكرة ال rbc's بنسميها erythrocyte و
+
+135
+00:10:44,650 --> 00:10:48,430
+أيضا هي كلمة يونانية ال erythro معناها red و ال
+
+136
+00:10:48,430 --> 00:10:54,740
+site معناها cell، تمام؟طيب مبدأ اعد خلايا الدم
+
+137
+00:10:54,740 --> 00:10:58,320
+البيضاء كيف احنا بنعد خلايا الدم البيضاء؟ في
+
+138
+00:10:58,320 --> 00:11:03,040
+البداية في عندي free-flowing capillary او ممكن
+
+139
+00:11:03,040 --> 00:11:06,040
+استخدم well-mixed anticoagulated venous blood
+
+140
+00:11:08,000 --> 00:11:11,740
+بستخدم يعني باخد حجم معين من الدم و يكون في عندي
+
+141
+00:11:11,740 --> 00:11:15,640
+محلول daily you want انا بخلطه مع هذا الدم بحيث
+
+142
+00:11:15,640 --> 00:11:21,040
+انه هذا المحلول يخلصني من ال RBCs و يحفظلي ال YBCs
+
+143
+00:11:21,040 --> 00:11:25,820
+علشان انا اتمكن من عدهاباستخدام شريحة معينة مخصصة
+
+144
+00:11:25,820 --> 00:11:30,720
+للعد انا بحط السائل او المحلول اللي انا حضرته اللي
+
+145
+00:11:30,720 --> 00:11:34,700
+هو عبارة عن الدم ال diligent بحطه على هذه الشريحة
+
+146
+00:11:34,700 --> 00:11:39,760
+و ببدأ اعد على ال microscope تمام؟ هذه هي الفكرة
+
+147
+00:11:39,760 --> 00:11:43,560
+ببساطة خلينا نتعمق فيها اكتر و نشوف ايش تفاصيلها
+
+148
+00:11:43,560 --> 00:11:47,890
+العلميةfree-flowing capillary or well-mixed
+
+149
+00:11:47,890 --> 00:11:51,730
+anticoagulated venous blood is added to a specific
+
+150
+00:11:51,730 --> 00:11:56,390
+volume of the diluent in the thermopiped طبعا احنا
+
+151
+00:11:56,390 --> 00:12:00,970
+ممكن انا استخدم عينة من الوريد تمام و احطها في
+
+152
+00:12:00,970 --> 00:12:05,110
+tube إدتة يعني عليها anticoagulant تمام و تصير هذه
+
+153
+00:12:05,110 --> 00:12:08,930
+العينة اسمها well-mixed anticoagulated venous
+
+154
+00:12:08,930 --> 00:12:09,370
+blood
+
+155
+00:12:14,850 --> 00:12:17,990
+فى tube editor لإنه احنا لما بنسحب عين تدام فى ال
+
+156
+00:12:17,990 --> 00:12:22,370
+editor انا بحكيلكم اعمله good mixing تمام بنعمل
+
+157
+00:12:22,370 --> 00:12:29,290
+good mixing و بعد هيك طبعا انا حكينا انه هذا ال
+
+158
+00:12:29,290 --> 00:12:34,130
+blood ممكن انا استخدمه عشان اعمله dilution مع
+
+159
+00:12:34,130 --> 00:12:38,610
+diluentطيب في نوع اخر او مصدر اخر للعينة تبعتي
+
+160
+00:12:38,610 --> 00:12:42,590
+ممكن يكون هو عبارة عن free flowing capillary يعني
+
+161
+00:12:42,590 --> 00:12:46,490
+من ال skin puncture تمام من ال .. من الاصبع و
+
+162
+00:12:46,490 --> 00:12:49,930
+مباشرة اعبي capillary طب ال capillary هذه اش
+
+163
+00:12:49,930 --> 00:12:54,670
+خصائصها؟ الخصائصها اسمها؟هي Thoma Bibet خلينا
+
+164
+00:12:54,670 --> 00:12:58,710
+نستعرض مع بعض صورة لهاي ال Bibet اللي هو ال .. ال
+
+165
+00:12:58,710 --> 00:13:03,510
+.. ال capillary اللي إحنا بنستخدمها و نشوف إيش هي
+
+166
+00:13:03,510 --> 00:13:07,470
+خصائصها، هي مش capillary بمعنى capillary زي ال
+
+167
+00:13:07,470 --> 00:13:11,750
+capillary tubes اللي إنتوا شفتوهملكن هي من الداخل
+
+168
+00:13:11,750 --> 00:13:18,010
+تمام مصممة على إنه المحلول يتم تعبئته فيها
+
+169
+00:13:18,010 --> 00:13:21,890
+بالخاصية الشعرية يعني هي من الداخل رفيعة كتير ولو
+
+170
+00:13:21,890 --> 00:13:25,810
+انها ممكن تكون سميكة من برا تمام؟ لكن من جوا رفيعة
+
+171
+00:13:25,810 --> 00:13:31,010
+كتيرتمام؟ في عندي طبعا واحدة بتكون مخصصة لعد ال
+
+172
+00:13:31,010 --> 00:13:35,510
+RPCs وواحدة مخصصة لعد ال white PCs احنا اليوم راح
+
+173
+00:13:35,510 --> 00:13:39,950
+نذكر اللي احنا بصدد نتعرف عليها اللي هي ال white
+
+174
+00:13:39,950 --> 00:13:44,070
+PCs ثومة بايبت لما نشرح ال RPCs count راح نتطرق ل
+
+175
+00:13:44,070 --> 00:13:49,740
+ال RPCs by bitطيب زي ما انتوا شايفين عبارة عن
+
+176
+00:13:49,740 --> 00:13:54,060
+ثومابايبت مكونة من هنا اسمها capillary stem stem
+
+177
+00:13:54,060 --> 00:14:00,360
+تمام؟ capillary stem و هذا ال capillary stem درج
+
+178
+00:14:00,360 --> 00:14:07,460
+لنص و واحد تمام؟ طيب في عندى هنا bulb مستودع او
+
+179
+00:14:07,460 --> 00:14:12,280
+انتفاخ تمام؟ مستودع او انتفاخ بسميه bulb هي هنا
+
+180
+00:14:12,280 --> 00:14:18,170
+capillary stemستم فيه من جوا يعني مصمم ان هو
+
+181
+00:14:18,170 --> 00:14:23,050
+المحلول يتم تعبقته فيه بالخاصية الشعرية تمام؟ لإنه
+
+182
+00:14:23,050 --> 00:14:30,910
+رفيع كتير و المدرج تمام؟ و في عندي أيضا مستودع هذا
+
+183
+00:14:30,910 --> 00:14:36,710
+المستودع بسميه bulb تمام؟ و هو عبارة عن انتفاخ في
+
+184
+00:14:36,710 --> 00:14:41,150
+داخله فيه قطعة القطعة هاي معدنية كرة معدنية بسميها
+
+185
+00:14:41,150 --> 00:14:48,870
+bead تمام؟ بسميها beadو أخر تدريج لأ ال .. ال ..
+
+186
+00:14:48,870 --> 00:14:55,090
+capillary طبعا بيكون عند 11 تمام؟ يعني نص و واحد
+
+187
+00:14:55,090 --> 00:14:58,870
+تمام؟ هذا ال .. ال .. هنا volume كبير بيكون موجود
+
+188
+00:14:58,870 --> 00:15:03,530
+فيه تمام؟ يعني تقريبا ال .. ال .. العشرة كلهم
+
+189
+00:15:03,530 --> 00:15:07,070
+موجودين هنا و على نهاية ال bulb بيكون في تدريج ..
+
+190
+00:15:07,070 --> 00:15:11,890
+رقم 11 تمام؟ بيكون في رقم 11هنا هاي الأشياء
+
+191
+00:15:11,890 --> 00:15:17,590
+التفاصيل .. هلأ راحت لزمني بشرح ال .. طريقة تعبئة
+
+192
+00:15:17,590 --> 00:15:23,170
+ال capillary و طريقة الحصول على الحسابات فإذا هذا
+
+193
+00:15:23,170 --> 00:15:28,450
+هو شكل ال white pieces بايبت تمام؟ أو ال white
+
+194
+00:15:28,450 --> 00:15:33,090
+pieces thermopiped خلونا نرجع لمحاضرتنا إذا حكينا
+
+195
+00:15:33,090 --> 00:15:37,270
+طبعا أنا حكينا ممكن هادي ال capillary أنه مباشرة
+
+196
+00:15:38,680 --> 00:15:42,780
+تمتع بيئتها لحد النص اللى عند علامة النص بال blood
+
+197
+00:15:42,780 --> 00:15:47,600
+تمام؟ او ممكن من ال skin puncture طبعا او ممكن من
+
+198
+00:15:47,600 --> 00:15:51,900
+ال tube ادتها انا سحبت منه .. سحبت يعني دم وريدي
+
+199
+00:15:51,900 --> 00:15:56,460
+وفضيته في tube ادتها وعملت ال will mixing تمام؟
+
+200
+00:15:56,460 --> 00:15:56,800
+طيب
+
+201
+00:16:02,850 --> 00:16:07,710
+the diluent أنا بستخدم diluent lies erythrocyte
+
+202
+00:16:07,710 --> 00:16:11,350
+بيكسرلي ال erythrocyte علشان ال erythrocyte عددها
+
+203
+00:16:11,350 --> 00:16:14,770
+كتير كبير بالملايين تمام لكن عدد ال white pieces
+
+204
+00:16:14,770 --> 00:16:19,570
+بالآلاف فإذا أنا لازم أكسر هاي ال erythrocyte عشان
+
+205
+00:16:19,570 --> 00:16:23,410
+ما تعملي noise و ما تأثر على القراءة أنا بدي بس ال
+
+206
+00:16:23,410 --> 00:16:26,070
+leucocyte اللي ماتكون موجودة علشان أعرف أقرأها
+
+207
+00:16:26,340 --> 00:16:30,060
+تمام؟ فوجود ال erythrocyte لإنه عددها كبير ممكن
+
+208
+00:16:30,060 --> 00:16:34,840
+يعيق عملية القراءة فإذا لازم أستخدم محلول من خواصه
+
+209
+00:16:34,840 --> 00:16:39,520
+إنه بيكسرلي ال erythrocyte من خواصه إنه بيكسر ال
+
+210
+00:16:39,520 --> 00:16:46,810
+erythrocyte تمام؟the diluted blood is added to the
+
+211
+00:16:46,810 --> 00:16:51,830
+hematocytometer chamber هذا الدم المخفف أنا بضيفه
+
+212
+00:16:51,830 --> 00:16:55,090
+على حاجة اسمها hematocytometer chamber اللي هي
+
+213
+00:16:55,090 --> 00:17:01,290
+حكينا شريحة مخصصة للعد إلها خواص أو مميزات إنه
+
+214
+00:17:01,290 --> 00:17:08,260
+يعني هي تستخدم فقط لعد الخلاياطيب إذا العينة تبعتي
+
+215
+00:17:08,260 --> 00:17:11,680
+إيش هتكون إما edata anticoagulated venous blood
+
+216
+00:17:11,680 --> 00:17:16,980
+يعني دم وريدي موجود في تيوب edata أو حتى ممكن تكون
+
+217
+00:17:16,980 --> 00:17:21,540
+من ال skin puncture ولكن ال edata anticoagulated
+
+218
+00:17:21,540 --> 00:17:27,050
+blood طبعايعني سواء كان هو أو كان من ال capillary
+
+219
+00:17:27,050 --> 00:17:31,590
+يعني من ال .. ال venous فهم يعني تقريبا أفضل أنواع
+
+220
+00:17:31,590 --> 00:17:35,230
+يعني أستخدم إيديتا أفضل نوع anticoagulant ممكن
+
+221
+00:17:35,230 --> 00:17:39,810
+يكون موجود أو حتى من ال .. ال skin puncture مباشرة
+
+222
+00:17:39,810 --> 00:17:46,560
+إلى ال thermopipedطيب، إيش الأدوات و ال .. ال
+
+223
+00:17:46,560 --> 00:17:50,380
+reagents اللي ممكن أنا أستخدمها؟ طبعا إحنا حكينا
+
+224
+00:17:50,380 --> 00:17:54,000
+إنه أنا لازم عينة الدم أخففها، بدي أستخدم diluting
+
+225
+00:17:54,000 --> 00:17:58,180
+fluid طبعا ال diluting fluid هذا في عندي أنواع منه
+
+226
+00:17:58,340 --> 00:18:01,560
+ممكن استخدم ascetic acid بتركيز 2% volume to
+
+227
+00:18:01,560 --> 00:18:07,020
+volume وطبعا احنا أخدنا محاضرة ال dilution وشرحنا
+
+228
+00:18:07,020 --> 00:18:13,100
+كيف انا بحضر تركيز volume to volume 2% مثلا او 3%
+
+229
+00:18:13,100 --> 00:18:18,020
+حسب و هذا ممكن تسأله عنه فبترجعوا لمحاضرة ال
+
+230
+00:18:18,020 --> 00:18:21,520
+dilution و بتعرفوا كيف احنا بنحضر volume to volume
+
+231
+00:18:21,520 --> 00:18:27,440
+wet to wet او wet to volume تمام؟طيب، diluting
+
+232
+00:18:27,440 --> 00:18:32,600
+fluid ممكن يكون Acetic acid 2% volume to volume أو
+
+233
+00:18:32,600 --> 00:18:37,760
+HCl 1% volume to volume أو حتى ممكن يكون عندي نوع
+
+234
+00:18:37,760 --> 00:18:41,400
+تالت اللي هو الأكثر شيوعا واستخداما هو ال terg
+
+235
+00:18:41,400 --> 00:18:45,480
+solution و هو المحلول اللي احنا راح نستخدمه، تمام؟
+
+236
+00:18:45,480 --> 00:18:50,100
+في المختبر، مما مكون .. مكون من glacial acetic
+
+237
+00:18:50,100 --> 00:18:56,010
+acid، تمام؟و crystal violet صبغة اسمها crystal
+
+238
+00:18:56,010 --> 00:18:59,550
+violet و distilled water هلأ ال glacial acetic
+
+239
+00:18:59,550 --> 00:19:04,110
+acid حمض تمام وظيفته انه يكسرلي الخلايا بمساعدة
+
+240
+00:19:04,110 --> 00:19:08,530
+ايضا الصبغة كمان بتساعد على انها تخرب ال membrane
+
+241
+00:19:08,530 --> 00:19:14,580
+للخلايا فبسهول تكسرهال .. لcrystal violet راح
+
+242
+00:19:14,580 --> 00:19:18,100
+تصبغلي أنوية .. بما أنها صابغة راح تصبغ أنوية ال
+
+243
+00:19:18,100 --> 00:19:23,720
+white pieces فإذن أنا بإمكاني إن أنا أشوف الخلايا
+
+244
+00:19:23,720 --> 00:19:27,900
+.. تمام .. أنويتها من الداخل أو الخلايا من الداخل
+
+245
+00:19:27,900 --> 00:19:31,480
+مخدة اللون الأزرق اللي هو نوع اللون لcrystal
+
+246
+00:19:31,480 --> 00:19:36,940
+violet فإذن بإمكاني يعني أعدها بسهولة ب .. من خلال
+
+247
+00:19:36,940 --> 00:19:43,990
+إنه أنا أعرف إنها هي مخدة اللون تمام؟ طيبالأدوات
+
+248
+00:19:43,990 --> 00:19:49,690
+اللى بدي اياها محتاجة diluting fluid وحكينا راح
+
+249
+00:19:49,690 --> 00:19:55,730
+نستخدم في المختبر TERC ومحتاجة thoma white pipet
+
+250
+00:19:55,730 --> 00:20:01,830
+اللى هى المدرجة نص لواحد لحداشر حكينا ال RBC's
+
+251
+00:20:01,830 --> 00:20:05,570
+النوع بتاعى بتكون تدريجة نص واحد مية واحد وال bulb
+
+252
+00:20:05,570 --> 00:20:08,650
+تبعها كبير اكبر من ال white pieces زى ما انتوا
+
+253
+00:20:08,650 --> 00:20:11,750
+شايفين كنتوا فى الصورة هناال .. ال .. ال white
+
+254
+00:20:11,750 --> 00:20:16,510
+pieces ال .. الحجم تبعها أكبر، تمام؟ فهي عشان هيك
+
+255
+00:20:16,510 --> 00:20:22,110
+نهاية التدريج تبعها ل 101 و .. لإن عددها أكبر بكون
+
+256
+00:20:22,110 --> 00:20:26,430
+محتاجة أنه تتخفف أكتر عشان هيك ال .. ال .. التدريج
+
+257
+00:20:26,430 --> 00:20:32,160
+أكبر هناالتفاصيل هاي رح ناخدها في ال RBCs count
+
+258
+00:20:32,160 --> 00:20:37,280
+lecture ولكن احنا حاليا رح نحكي فقط عن ال
+
+259
+00:20:37,280 --> 00:20:41,080
+thermopipet اللي هي خاصة بعد ال white pieces حكينا
+
+260
+00:20:41,080 --> 00:20:45,940
+تدريجها نص و واحد و لعند 11 تمام محتاجة هذه ال
+
+261
+00:20:45,940 --> 00:20:49,700
+thermopipet محتاجة ال hematocytometer chamber اللي
+
+262
+00:20:49,700 --> 00:20:53,580
+هي الشريحة المخصصة للعدمحتاجة cover slip اللي هي
+
+263
+00:20:53,580 --> 00:20:56,340
+القطعة الزجاجية اللي أنا بحطها على الشريحة علشان
+
+264
+00:20:56,340 --> 00:21:01,440
+تغطيلي العينة و بإمكاني بعد هيك أنه أستخدم .. يعني
+
+265
+00:21:01,440 --> 00:21:05,220
+أحطها على ال microscope و أعد بلزمني microscope،
+
+266
+00:21:05,220 --> 00:21:10,260
+بلزمني wipe شاش أو جوز أو قطن، بلزمني برضه كمان
+
+267
+00:21:10,260 --> 00:21:15,120
+قطع أو .. قطن مغموسة بالكحول اللي هي alcohol pads
+
+268
+00:21:17,120 --> 00:21:19,960
+تمام، إذا إيش الأدوات اللي بتلزمني؟ حكينا
+
+269
+00:21:19,960 --> 00:21:24,540
+thomapipet، بيكون في عندي أيضا متصلة ب rubber، هذا
+
+270
+00:21:24,540 --> 00:21:28,160
+ال rubber بيكون يا بنات معقّم ونظيف ومتصل بقطعة
+
+271
+00:21:28,160 --> 00:21:33,480
+عشان احنا أحيانا بنستخدم الفم في سحب المحلولو أيضا
+
+272
+00:21:33,480 --> 00:21:37,660
+في عندي هنا hematocytometer chamber اللي هو عبارة
+
+273
+00:21:37,660 --> 00:21:43,120
+عن شريحة مخصصة للعدد شايفين انه مش زي الشرائح
+
+274
+00:21:43,120 --> 00:21:46,620
+العادية الزجاجية العادية البسيطة هي شريحة مصممة
+
+275
+00:21:46,620 --> 00:21:51,300
+بطريقة معينة تمكنني من عدد الخلايا و حساب العدد
+
+276
+00:21:51,300 --> 00:21:56,480
+بطريقة accurate تمام؟ فإذا هذه هي الإدوات اللي
+
+277
+00:21:56,480 --> 00:22:00,800
+بتلزمنيطبعا اللي هي metacitometer ال chamber بيكون
+
+278
+00:22:00,800 --> 00:22:05,720
+موجود في plastic container خاص فيه عشان احنا نحافظ
+
+279
+00:22:05,720 --> 00:22:09,220
+عليه ما ينكسر لإنه بنرجع بنستخدمه مش زي الشريحة
+
+280
+00:22:09,220 --> 00:22:13,640
+العادية استخدمته حطيت عليه مسحة دم مثلا بعد ما
+
+281
+00:22:13,640 --> 00:22:18,230
+أخلص بكبها لأفي عندي هذا يعني أنا بعقمه و برجع
+
+282
+00:22:18,230 --> 00:22:23,230
+بستخدمه لأكتر من عينة لإنه يعني هو غالي الثمن و
+
+283
+00:22:23,230 --> 00:22:28,090
+مابنفع احنا نتلفه و لازم نحافظ عليه من الكثر فإذا
+
+284
+00:22:28,090 --> 00:22:31,560
+هو بيكون موجود في plastic containerالـ
+
+285
+00:22:31,560 --> 00:22:35,920
+hematocytometer تفاصيل أكثر عن هذا الـ chamber الـ
+
+286
+00:22:35,920 --> 00:22:39,100
+hematocytometer counting chamber is used for cell
+
+287
+00:22:39,100 --> 00:22:42,960
+counting هو عبارة عن .. احنا بنسمي hematocytometer
+
+288
+00:22:42,960 --> 00:22:47,380
+chamber .. chamber ولكن هو عبارة عن شريحة مخصصة
+
+289
+00:22:47,380 --> 00:22:51,500
+لعد الخلايا مثل ما ذكرنا it's constructed so that
+
+290
+00:22:51,500 --> 00:22:54,220
+the distance between the bottom of the cover slip
+
+291
+00:22:54,220 --> 00:22:57,840
+and the surface of the counting area point one
+
+292
+00:22:57,840 --> 00:23:02,300
+millimeterهو عبارة عن مصمم بطريقة معينة بحيث ان لو
+
+293
+00:23:02,300 --> 00:23:05,620
+حطيت عليه cover slip اللي هو الغطاء الزجاجي اللي
+
+294
+00:23:05,620 --> 00:23:09,500
+انا بحط عليه تمام المسافة هتكون ما بين ال cover
+
+295
+00:23:09,500 --> 00:23:13,860
+slip و ال chamber هتكون مسافة point واحد
+
+296
+00:23:13,860 --> 00:23:18,320
+millimeterتمام؟ اللي هي يعني ال .. ال .. المنطقة
+
+297
+00:23:18,320 --> 00:23:21,860
+أو المسافة اللي أنا بعبي فيها العينة ارتفاع العينة
+
+298
+00:23:21,860 --> 00:23:25,800
+هيكون point واحد millimeter لإنها هتكون موجودة في
+
+299
+00:23:25,800 --> 00:23:29,880
+ال .. المنطقة مابين ال chamber و ال cover slab the
+
+300
+00:23:29,880 --> 00:23:33,300
+surface of that chamber contain two square ruled
+
+301
+00:23:33,300 --> 00:23:38,240
+areas separated by at shape mode خليني أشوفزي ما
+
+302
+00:23:38,240 --> 00:23:42,880
+انتوا شايفين هذا هو ال chamber تمام؟ زي عامل شكل
+
+303
+00:23:42,880 --> 00:23:47,580
+الأتشاب وين احنا بنعد؟ في عندي هنا مربع و هنا مربع
+
+304
+00:23:47,580 --> 00:23:52,540
+و بكونوا مسطرين ه .. تسطيرة معينة هورجيكم إياها
+
+305
+00:23:52,540 --> 00:23:56,660
+بالعين المجردة أول ما نرجع على المختبر على شأع
+
+306
+00:23:56,660 --> 00:24:01,960
+أساس أنه تشوفوها منيحطبعا لو أنا جيت حطيت cover
+
+307
+00:24:01,960 --> 00:24:06,400
+slip قطعة زجاجية cover على هذا ال chamber طبعا أنا
+
+308
+00:24:06,400 --> 00:24:10,780
+بحط ال cover slip بعدين بعب العينة طيب فإذا لو أنا
+
+309
+00:24:10,780 --> 00:24:14,140
+حطيت ال cover slip راح يكون في عندي مسافة فاصلة
+
+310
+00:24:14,140 --> 00:24:17,540
+هنا بين ال .. بين ال chamber و ال cover slip هذه
+
+311
+00:24:17,540 --> 00:24:20,820
+المسافة الفاصلة ارتفاعها واحد من عشرة millimeter
+
+312
+00:24:20,820 --> 00:24:25,960
+هنا تكمن العينة او يعني هنا انا بتم .. ب .. بعمل
+
+313
+00:24:25,960 --> 00:24:30,170
+تعبي على العينةبعبي العينة في هذه المنطقة طبعا
+
+314
+00:24:30,170 --> 00:24:33,510
+لازم انا لما اعبي العينة تكون العينة فقط على
+
+315
+00:24:33,510 --> 00:24:37,630
+المربع يعني حوافها جاي على حواف المربع بالظبط
+
+316
+00:24:37,630 --> 00:24:42,210
+مابنفع ان العينة تكون خارج عن حواف المربع زي هذا
+
+317
+00:24:42,210 --> 00:24:46,070
+الشكل او حتى فيها air bubble فيها فقعات يعني
+
+318
+00:24:46,070 --> 00:24:51,350
+فراغات لإن هذا هيأثر على الحسابات طبعتي فهيكون في
+
+319
+00:24:51,350 --> 00:24:55,710
+عندي خطأ error اذا لازم انا اعبيها بشكل ان مايكون
+
+320
+00:24:55,710 --> 00:25:02,000
+فيهافراغات إنها ما تطف على الجوانب لازم تكون فقط
+
+321
+00:25:02,000 --> 00:25:06,340
+على حواف المربع اللي مخصص للعدد هذا المربع و هذا
+
+322
+00:25:06,340 --> 00:25:12,860
+تمام و تكون يعني حجمها مناسب و كميتها مناسبة تمام
+
+323
+00:25:12,860 --> 00:25:17,760
+و ما تكون طبعا يعني إنها هيك عاملة زي التفاف دائرة
+
+324
+00:25:17,760 --> 00:25:20,960
+إنه يعني الحجم ناقص لأ لازم تكون well-fit يعني
+
+325
+00:25:20,960 --> 00:25:25,720
+تماما مخدة شكل المربع اللي هي جالسة فيهه .. هنا ..
+
+326
+00:25:25,720 --> 00:25:29,080
+هذا شكل ايه؟ او يعني التعليمات اللي خاصة بالتعبئة
+
+327
+00:25:29,080 --> 00:25:34,200
+طبعا احنا بنعد .. ممكن نعد في مربع واحد في واحد
+
+328
+00:25:34,200 --> 00:25:37,760
+منهم و ممكن اعد في المربعين و في نهاية القراءة
+
+329
+00:25:37,760 --> 00:25:41,140
+اقسمها على اتنين اخد ال average لإلها اقسمها على
+
+330
+00:25:41,140 --> 00:25:44,660
+اتنين تمام؟ طيب
+
+331
+00:25:47,820 --> 00:25:51,640
+إيش ال .. التجربة طبعتي؟ أول حاجة سواء كان أنا
+
+332
+00:25:51,640 --> 00:25:55,040
+بستخدم معايا ال blood من skin puncture يعني من
+
+333
+00:25:55,040 --> 00:26:00,040
+الإصبع أو من edita tube يعني سحبت .. سحبت من وريد
+
+334
+00:26:00,040 --> 00:26:03,640
+وقع ملقته في ال .. في edita tube عملت mixing منيح
+
+335
+00:26:03,640 --> 00:26:08,730
+مع ال editaسواء كانت العينة skin puncture أو vein
+
+336
+00:26:08,730 --> 00:26:14,210
+puncture أنا لازم أملأ ال .. ال .. ال .. ال .. ال
+
+337
+00:26:14,210 --> 00:26:14,490
+.. ال .. ال .. ال .. ال .. ال .. ال .. ال .. ال ..
+
+338
+00:26:14,490 --> 00:26:15,030
+ال .. ال .. ال .. ال .. ال .. ال .. ال .. ال .. ال
+
+339
+00:26:15,030 --> 00:26:15,590
+.. ال .. ال .. ال .. ال .. ال .. ال .. ال .. ال ..
+
+340
+00:26:15,590 --> 00:26:16,330
+ال .. ال .. ال .. ال .. ال .. ال .. ال .. ال .. ال
+
+341
+00:26:16,330 --> 00:26:16,870
+ال .. ال .. ال .. ال .. ال .. ال .. ال .. ال .. ال
+
+342
+00:26:16,870 --> 00:26:19,630
+.. ال .. ال .. ال .. ال .. ال .. ال .. ال .. ال ..
+
+343
+00:26:19,630 --> 00:26:19,690
+ال .. ال .. ال .. ال .. ال .. ال .. ال .. ال .. ال
+
+344
+00:26:19,690 --> 00:26:21,850
+.. ال .. ال .. ال .. ال .. ال .. ال .. ال .. ال ..
+
+345
+00:26:21,850 --> 00:26:26,170
+ال .. ال .. ال ..الـ Cabillary أو الـ Thaumabibet
+
+346
+00:26:26,170 --> 00:26:29,890
+بالـ blood طبعا إذا أنا حطيتها في تيوب هتتبهدل من
+
+347
+00:26:29,890 --> 00:26:35,610
+برا فلازم أنا أمسحها ب wipe تمام عشان أنظفها أشيلي
+
+348
+00:26:35,610 --> 00:26:40,390
+ال excess من ال blood اللي موجودة على السطحة أو
+
+349
+00:26:40,390 --> 00:26:43,550
+عليها من برا تمام لإن هذا هيأثر على ال dilution
+
+350
+00:26:43,800 --> 00:26:47,460
+طمام؟ اذا اول حاجة املا ايه ال .. ال .. ال thoma
+
+351
+00:26:47,460 --> 00:26:52,320
+pipet لعند علابة النص بال blood بعدين امسحي wipe
+
+352
+00:26:52,320 --> 00:26:55,020
+the outside of the capillary بنمسحي ال capillary
+
+353
+00:26:55,020 --> 00:26:57,920
+من برا علشان ال excess blood اللي موجودة عليها من
+
+354
+00:26:57,920 --> 00:27:02,640
+برا ممكن يأثر على ال dilution طمام؟ ليش؟ لإن انا
+
+355
+00:27:02,640 --> 00:27:05,960
+هاي ال capillary بعد ما املأها بال blood بدي احطها
+
+356
+00:27:05,960 --> 00:27:10,820
+بشكل عمودي في tube و هذا ال tube موجود فيه diluent
+
+357
+00:27:10,820 --> 00:27:16,080
+طمام؟و .. و أصحى بال diligent ام لأ ال .. ال
+
+358
+00:27:16,080 --> 00:27:20,080
+capillary بال diligent لعند العلامة 11 تمام؟ لعند
+
+359
+00:27:20,080 --> 00:27:26,410
+العلامة 11 طيب اش اللي راح يصير بالظبط؟خلّينا نرجع
+
+360
+00:27:26,410 --> 00:27:30,110
+لشكل الكابلري عشان نشرح عليها أنا ملاقت لعند
+
+361
+00:27:30,110 --> 00:27:33,970
+العلامة هنا نص blood مسحتها و نظفتها من برا عشان
+
+362
+00:27:33,970 --> 00:27:37,030
+ال excess blood ممكن يؤثر على ال dilution بعد هيك
+
+363
+00:27:37,030 --> 00:27:40,870
+باخد الكابلري هادي بشكل عمودي بفوت في محلول تمام
+
+364
+00:27:40,870 --> 00:27:45,050
+المحلول هذا عبارة عن diluent تمام it's a solution
+
+365
+00:27:45,050 --> 00:27:51,490
+حكيناال .. رح أسحب او يعني رح ا .. ال .. ال ..
+
+366
+00:27:51,490 --> 00:27:56,450
+أكمل ل .. في .. تعبقت الثومة بايبت، بدلي one لعندي
+
+367
+00:27:56,450 --> 00:28:01,890
+العلامة 11، تمام؟طيب، أش اللي راح يصير؟ ال blood
+
+368
+00:28:01,890 --> 00:28:06,330
+هنا لما ييجي عب التارك ال blood هيطلع .. كله
+
+369
+00:28:06,330 --> 00:28:10,910
+هيستقر وين؟ هيستقر في منطقة المستودع، يعني هنا ال
+
+370
+00:28:10,910 --> 00:28:15,210
+blood هيكون موجود، تمام؟ و هنا هيصير فقط موجود
+
+371
+00:28:15,210 --> 00:28:20,370
+تارك و هنا موجود أيضا بقايا ال blood مع ال ..
+
+372
+00:28:20,370 --> 00:28:27,320
+التاركطيب بعد ما أملأ لحد العلامة 11 بجيب قطنة و
+
+373
+00:28:27,320 --> 00:28:32,540
+بفضّي محتويات ال stem هنا اللي هي إيش صارت موجود
+
+374
+00:28:32,540 --> 00:28:36,940
+فيها؟ موجود فيها فقط تارك، تمام؟ فقط موجود فيها
+
+375
+00:28:36,940 --> 00:28:40,420
+تارك solution اللي هو ال daily one لإن الدم خلاص
+
+376
+00:28:40,420 --> 00:28:48,330
+صعد إلى منطقة المستودعطيب إذا بعد ملق ال capillary
+
+377
+00:28:48,330 --> 00:28:57,410
+لحد العلامة 11 طبعا لازم أسكرها هيك بعد ما أطلعها
+
+378
+00:28:57,410 --> 00:29:01,170
+من ال daily want بسكرها بالسبابة لإن أي فتحة فيها
+
+379
+00:29:01,170 --> 00:29:05,390
+ممكن يرجع يكب المحلول منها طبعا لازم أعملها مغلقة
+
+380
+00:29:05,390 --> 00:29:11,450
+و بعد هيك بروح على قطنة على قطعة قطن بفضي محتويات
+
+381
+00:29:11,450 --> 00:29:16,670
+الاستمال هي الواحدة هذه طبعايعني بفضّي تقريبا تلت
+
+382
+00:29:16,670 --> 00:29:21,490
+قطرات أو أربع قطرات اللي هم محتويات الستم و بعد
+
+383
+00:29:21,490 --> 00:29:26,770
+هيك بروح بمسح ال .. ال hematocytometer و بنضفه
+
+384
+00:29:26,770 --> 00:29:33,930
+بقالكحول بعد بقطعة قطنية أو قمع .. يعني جوز شاش
+
+385
+00:29:33,930 --> 00:29:39,630
+مبلل بيه الكحولبنطفه بحط عليه cover slip و
+
+386
+00:29:39,630 --> 00:29:44,310
+باستخدام ال by bit من عن الطرف هنا من عند حواف أو
+
+387
+00:29:44,310 --> 00:29:49,510
+حافة ال cover slip بسير افرغ محلول بحيث انه يملأ
+
+388
+00:29:49,510 --> 00:29:56,630
+المربع و يعبيه بشكل حكينا متناسق انه ما يطف من عن
+
+389
+00:29:56,630 --> 00:29:59,930
+الجوانب مايكون excess فيه عندي و مايكون فيه فراغات
+
+390
+00:29:59,930 --> 00:30:04,790
+او حتى airdrops اللي هي يعني فقعة هوا
+
+391
+00:30:07,660 --> 00:30:12,480
+مجرد ما أنا .. ما لاقت ال chamber بروح بحطه على ال
+
+392
+00:30:12,480 --> 00:30:17,320
+microscope أول إشي على عشرة، تمام؟ ممكن أجيب ال
+
+393
+00:30:17,320 --> 00:30:20,680
+field على أربعة، بعدين أروح أحط على عشرة علشان
+
+394
+00:30:20,680 --> 00:30:24,640
+أعد، تمام؟ و بعد أنا على العدسة رقم عشرة، تمام؟
+
+395
+00:30:24,640 --> 00:30:28,260
+إذا لازم أجيب ال field على أربعةطيب لإنه إحنا
+
+396
+00:30:28,260 --> 00:30:30,820
+حاكينا .. اتعلمنا إنه أنا بجيب ال field أول إشي
+
+397
+00:30:30,820 --> 00:30:36,100
+على عدسة تكبيرها صغير بعدين بكبر على العدسة اللي
+
+398
+00:30:36,100 --> 00:30:39,120
+أنا مراد استخدامها أو العدسة اللي تكبيرها أكبر
+
+399
+00:30:39,120 --> 00:30:45,640
+شويةبب .. طبعا كيف ال .. ال .. شكل ال .. ال field
+
+400
+00:30:45,640 --> 00:30:50,860
+اللي هجيبه؟ هشوفه بهذا الشكل، إشي مسطر، تمام؟ طيب،
+
+401
+00:30:50,860 --> 00:30:54,500
+إحنا حكينا إنه ال .. ال .. ال chamber إله تستيرة
+
+402
+00:30:54,500 --> 00:30:59,640
+معينة، تمام؟ و التستيرة هذه أنا هورجيكم إياها على
+
+403
+00:30:59,640 --> 00:31:03,600
+ال .. يعني هتشوفوها بالعين المجردة، ولكن إحنا ما
+
+404
+00:31:03,600 --> 00:31:07,260
+راح نشوف تفاصيلها، تفاصيل هذه التستيرة راح تكون
+
+405
+00:31:07,260 --> 00:31:11,720
+واضحة أكتر على ال microscopeال .. التصطيرة بهذا
+
+406
+00:31:11,720 --> 00:31:17,940
+الشكل أنا في عندى يعني خطوط مضغوطة تمام؟ خطوط
+
+407
+00:31:17,940 --> 00:31:23,780
+مضغوطة بهذا الشكل تمام؟ متقاطعة وفي على الحواف
+
+408
+00:31:23,780 --> 00:31:27,660
+مربعات تمام؟ أربع مربعات في المنطقة الوحدة يا بنات
+
+409
+00:31:27,660 --> 00:31:30,820
+يعني أنا لو جيت على ال chamber من هنا في المنطقة
+
+410
+00:31:30,820 --> 00:31:35,240
+الوحدةتمام؟ هنا موجود نفس التصطيرة اللي في ال
+
+411
+00:31:35,240 --> 00:31:38,400
+slide السابق اللي .. اللي كان .. من شوية كنا فيها
+
+412
+00:31:38,400 --> 00:31:42,300
+و هنا أيضا موجود نفس التصطيرة حكينا أنا بعين ممكن
+
+413
+00:31:42,300 --> 00:31:45,080
+أقعد هنا لحال أو أقعد هنا لحال أو ممكن أقعد في
+
+414
+00:31:45,080 --> 00:31:49,600
+الجهتين و أقسم على اتنين تمام؟
+
+415
+00:31:51,820 --> 00:32:01,160
+طيب إذا حكينا إنه هيك التستيرة مضغوط يعني زي ال ..
+
+416
+00:32:01,160 --> 00:32:06,200
+ال cross هيك المضغوط تمام؟ و يعني .. مش يعني مضغوط
+
+417
+00:32:06,200 --> 00:32:10,100
+يعني ال .. الستور أو الخطوط جنب بعضها متلاسقة يعني
+
+418
+00:32:10,100 --> 00:32:14,590
+مافي مسافات، شايفين؟ جنب بعضهاو هنا أيضا مضغوط
+
+419
+00:32:14,590 --> 00:32:18,910
+فهذا التقاطع المضغوط على أطرافه موجود عندي أربع
+
+420
+00:32:18,910 --> 00:32:22,950
+مربعات الأربع مربعات هذه هي اللي بنعد فيها ال
+
+421
+00:32:22,950 --> 00:32:25,590
+white pieces شايفين علامة ال W هذه يعنيها هذه
+
+422
+00:32:25,590 --> 00:32:29,570
+مخصصة لل white pieces لعد ال white pieces تمام ال
+
+423
+00:32:29,570 --> 00:32:34,790
+R هذه مخصصة لعد ال RPCs راح نشوف تفاصيلها في
+
+424
+00:32:34,790 --> 00:32:41,540
+محاضرة ال RPCs count تمام؟هذه المربعات عبارة عن
+
+425
+00:32:41,540 --> 00:32:46,040
+ستاشر مربع أربعة في أربعة تمام؟ هي اللي أنا بعد
+
+426
+00:32:46,040 --> 00:32:49,860
+فيها طيب حوافها من هنا بتكون مستطيلات على الأطراف
+
+427
+00:32:49,860 --> 00:32:52,400
+هنا مستطيلات يعني هو مش جايب ليها موضح ليها بس
+
+428
+00:32:52,400 --> 00:32:58,600
+أنتوا بتشوفوها في ال .. تحت ال microscope في عندي
+
+429
+00:32:58,600 --> 00:33:06,890
+مستطيلات هنا تمام؟طيب فإذا أنا بعد في هذه المربعات
+
+430
+00:33:06,890 --> 00:33:12,190
+الأربعة، تمام؟ في جهة واحدة ممكن أعد أيضا كمان
+
+431
+00:33:12,190 --> 00:33:17,090
+أربعة في الجهة التانية من ال at shed من ال .. ال
+
+432
+00:33:17,090 --> 00:33:22,310
+chamberو يكون أنا هيك عديت في 8 مربعات أربعة فوق
+
+433
+00:33:22,310 --> 00:33:26,130
+أربعة تحت فبقسم على اتنين لإنه في النهاية أنا بدي
+
+434
+00:33:26,130 --> 00:33:30,130
+قراة لأربع مربعات أطبق فيها في القانون سواء عديت
+
+435
+00:33:30,130 --> 00:33:33,530
+في أربعة من جهة واحدة أو عديت في تمانية و قسمت على
+
+436
+00:33:33,530 --> 00:33:37,610
+اتنين مش مهم المهم أنا يعني بدي في النهاية مجموع
+
+437
+00:33:37,610 --> 00:33:43,890
+القراة يكون لأربع مربعات طيبإذا زي ما أنتوا شايفين
+
+438
+00:33:43,890 --> 00:33:49,830
+المنطقة المضغوطة هذه بتفيدني علشان أعرف أنا في أين
+
+439
+00:33:49,830 --> 00:33:53,870
+في أي مربع تمام؟ يعني انت مثلا لو كانت ال .. ال ..
+
+440
+00:33:53,870 --> 00:33:57,710
+طبعا هي بنت ال field بيكون بس مسلط على منطقة معينة
+
+441
+00:33:57,710 --> 00:34:01,090
+زي هيك فانت لو عرفت .. لو شوفت المضغوط من هذه
+
+442
+00:34:01,090 --> 00:34:04,950
+الجهة و من هذه الجهة مضغوط فانت معناها موجودة في
+
+443
+00:34:04,950 --> 00:34:08,490
+المربع الأول في المربع التاني لما تكون المضغوط
+
+444
+00:34:08,490 --> 00:34:12,650
+بهذا الشكلو في المربع التالت لما تكون .. يكون
+
+445
+00:34:12,650 --> 00:34:15,470
+المضغوط بهذا الشكل و في المربع الرابع لما يكون
+
+446
+00:34:15,470 --> 00:34:19,570
+بهذا الشكل تمام؟ فإذا إنت when كدك تعرفي حالك في
+
+447
+00:34:19,570 --> 00:34:23,230
+أي مربع مثلا كنت هنا في أي مربع في .. مثلا كنت هنا
+
+448
+00:34:23,230 --> 00:34:27,210
+و شفتي إنه مضغوط على الجانب من هنا و من .. من تحت
+
+449
+00:34:27,210 --> 00:34:30,510
+فمعناها بدك تتخيل الرسمة كلها و تعرفي حالك إنك إنت
+
+450
+00:34:30,510 --> 00:34:34,230
+في المربع التاني لو مثلا لإنه طبعا هو ما بعرضلك كل
+
+451
+00:34:34,230 --> 00:34:38,430
+الرسمة هذه ال field ال field بيكون مركز على ال 16
+
+452
+00:34:38,430 --> 00:34:42,470
+مربع اللي هناتمام؟ و جايبلك .. بس عرضلك جزء من
+
+453
+00:34:42,470 --> 00:34:46,490
+المضغوط اللي محيط في ال 16 مربع اللي انت بتقرأ
+
+454
+00:34:46,490 --> 00:34:54,130
+فيهم تمام؟ ف .. وين اتجاه المضغوط بتتخيلي انت في
+
+455
+00:34:54,130 --> 00:34:59,230
+مخيلتك كيف الرسمة او الشكل بشكل مكبر و بتعرفي حالك
+
+456
+00:34:59,230 --> 00:35:02,390
+انت وين بالظبط ف .. و بتعرفي حالك من وين .. وين
+
+457
+00:35:02,390 --> 00:35:05,330
+بدك تتنقلي يعني انت مثلا في المربع الأول شوفت
+
+458
+00:35:05,330 --> 00:35:08,970
+المضغوط هنا و هنا معناها انت في المربع الأولوين
+
+459
+00:35:08,970 --> 00:35:12,310
+بدك تنتقلي؟ بدك تنتقلي للتاني فمعناها مثلا بدك
+
+460
+00:35:12,310 --> 00:35:17,130
+تتحركي بهذا الاتجاه في ال field طبعا او بدك تنزلي
+
+461
+00:35:17,130 --> 00:35:21,310
+هيك عشان تروحي على التالت فإذا بيساعدك وين ..
+
+462
+00:35:21,310 --> 00:35:26,970
+تحددي وين انت موجودة و كيف بدك تنتقلي لأي مربع و
+
+463
+00:35:26,970 --> 00:35:32,610
+كيف الاتجاه تبع حركتكتمام؟ هذه التفاصيل كلها راح
+
+464
+00:35:32,610 --> 00:35:37,430
+نشتغلها خطوة بخطوة مع بعض فيعني اذا في اشي مبهم او
+
+465
+00:35:37,430 --> 00:35:42,070
+اشي تساؤلات كيف احنا بنعمل هاي الشغلة حاولوا انه
+
+466
+00:35:42,070 --> 00:35:48,090
+يعني تكونوا relaxed و ما .. يعني ما تستغربوا او
+
+467
+00:35:48,090 --> 00:35:51,550
+تخافوا لإنه الاشي راح يكون ع التطبيق ان شاء الله
+
+468
+00:35:52,450 --> 00:35:56,310
+مابحكيش ان هو هيكون سهل جدا ممكن يكون في صعوبة
+
+469
+00:35:56,310 --> 00:35:59,510
+ولكن يمكن تجاوزها و راح تتعلموا و تكون ان شاء الله
+
+470
+00:35:59,510 --> 00:36:05,830
+متمكنين و شطرين طيب احنا بس في النهاية بدي اذكر
+
+471
+00:36:05,830 --> 00:36:08,550
+انه انا لما بتحرك بهذا الاتجاه يعني انا بتحرك
+
+472
+00:36:08,550 --> 00:36:12,790
+لليسار في الشريحة بكون بتحرك لليمين على ال chamber
+
+473
+00:36:12,790 --> 00:36:16,030
+لإن الحركة بتكون معاكوسة و مقلوبة ايضا الصورة
+
+474
+00:36:16,030 --> 00:36:20,230
+بتكون معاكوسة و مقلوبةتمام طيب إيش اللي أنا بده
+
+475
+00:36:20,230 --> 00:36:24,330
+أعده في ال 16 مربع هدول؟ بده أعد النقاط السودة هاي
+
+476
+00:36:24,330 --> 00:36:29,010
+طب كيف أنا أعرف هذه النقطة السودة؟ خلية ولا دبرس و
+
+477
+00:36:29,010 --> 00:36:36,030
+غبار أو مثلا أشياء موسخة الشريحة ممكن تكون يعني
+
+478
+00:36:36,030 --> 00:36:40,020
+إشي مش خلايا كيف أنا بده أعرفه؟زاكرين تركيب ال
+
+479
+00:36:40,020 --> 00:36:43,300
+microscope لما حكينا انه ال course adjustment عشان
+
+480
+00:36:43,300 --> 00:36:46,020
+اجيب ال field عشان اقرب المسافة ما بين ال stage و
+
+481
+00:36:46,020 --> 00:36:49,900
+العدسات و ال fine adjustment علشان اشوف ال details
+
+482
+00:36:49,900 --> 00:36:54,660
+التفاصيل تمام فانا بلعب بال fine adjustment إذا
+
+483
+00:36:54,660 --> 00:36:58,540
+ظهرت إلي في داخل الخلية هذه في داخل النقطة السودة
+
+484
+00:36:58,540 --> 00:37:02,320
+لون أزرق تمام أو حتى أخضر يعني هو ممكن يكون على
+
+485
+00:37:02,320 --> 00:37:05,600
+اخضرار لأ بسبب إضاءة ال microscope بس هو فعليا
+
+486
+00:37:05,600 --> 00:37:11,390
+الصبغة لونها أزرق تمامفإذا ظهر عندى لون فى الداخل
+
+487
+00:37:11,390 --> 00:37:14,610
+معناه عادي خليها ماخدة لون مصبوغة بالصبغة اللى
+
+488
+00:37:14,610 --> 00:37:18,030
+موجودة فى مكونات ال Turk solution فى مكونات ال
+
+489
+00:37:18,030 --> 00:37:24,580
+diligent اللى أنا استخدمتهاطيب الحساب او الحسابات
+
+490
+00:37:24,580 --> 00:37:30,300
+اللى احنا بدنا نجريها لحتى نجيب التراكيز او العدد
+
+491
+00:37:30,300 --> 00:37:32,820
+الخلايا ال white pieces count ال total white
+
+492
+00:37:32,820 --> 00:37:36,960
+pieces count طبعا انا عندي عمق العينة طبعتي اللى
+
+493
+00:37:36,960 --> 00:37:40,920
+هى المسافة اللى انا عبقت فيها او ملقت فيها العينة
+
+494
+00:37:40,920 --> 00:37:45,280
+كانت 1 من 10 ملي متر اللى هى المسافة الفاصلة ما
+
+495
+00:37:45,280 --> 00:37:50,840
+بين ال chamber و cover slipتمام؟ طيب ال .. ال
+
+496
+00:37:50,840 --> 00:37:54,500
+dilution اللي أنا بستخدمه أنا بعمل dilution واحد
+
+497
+00:37:54,500 --> 00:37:59,900
+لعشرين من أين أجا .. من أين أتى هذا الواحد لعشرين؟
+
+498
+00:37:59,900 --> 00:38:05,310
+خلينا نرجع لل Thaumabioticو نستفكر مع بعض احنا ايش
+
+499
+00:38:05,310 --> 00:38:10,830
+ذكرنا بخصوص ال Tom and pie أنا ملقت الدم لعند نص
+
+500
+00:38:10,830 --> 00:38:19,530
+تمام و بعد هيك كملت بال daily one لعند 11 يعني
+
+501
+00:38:19,530 --> 00:38:26,590
+كمان في عندي نرجع هنا نعمل
+
+502
+00:38:29,870 --> 00:38:35,010
+بنحط notes كان في عندي blood خدنا نكتب هنا بس ال
+
+503
+00:38:35,010 --> 00:38:45,050
+blood إلى diluent تمام؟ كان في عندي ال blood حطيت
+
+504
+00:38:45,050 --> 00:38:58,330
+أنا نص تمام؟ و ال .. ال diluent كان 11 تمام؟ طيبفي
+
+505
+00:38:58,330 --> 00:39:02,170
+عندى محتويات ال stem انا حكيت فضيتها اللى كانت
+
+506
+00:39:02,170 --> 00:39:05,510
+daily want لحاله، تمام؟ ذاكرين .. ذاكرين لما
+
+507
+00:39:05,510 --> 00:39:10,230
+حكيتلكم ال blood بيطلع ع المستودع و ال .. ال stem
+
+508
+00:39:10,230 --> 00:39:15,230
+اللى هى من صفر لواق من .. لعند الواحد هذه بتصير
+
+509
+00:39:15,230 --> 00:39:20,590
+daily want كامل او daily want صافىترك لحاله تمام و
+
+510
+00:39:20,590 --> 00:39:24,530
+أنا بفضّي محتويات هذا ال stem على قطعة قطن اللي هي
+
+511
+00:39:24,530 --> 00:39:28,230
+التلت أو أربع قطرات اللي أنا بتخلص منهم فأصبح أنا
+
+512
+00:39:28,230 --> 00:39:31,850
+خلصت .. اتخلصت من محتويات ال stem اللي هي عبارة عن
+
+513
+00:39:31,850 --> 00:39:41,210
+واحد يعني صار عندي ال dilution نص إلى .. إلى
+
+514
+00:39:41,210 --> 00:39:46,270
+عشرة اللي هي الواحد اللي خلصت منه
+
+515
+00:39:49,070 --> 00:39:53,110
+تمام؟ اتخلصت منه الواحد طب ليش انا عملت اصلا عبيته
+
+516
+00:39:53,110 --> 00:39:57,810
+لعند ال 11؟ لأ لإنه انا معنية انه يعني بهذه ال ..
+
+517
+00:39:57,810 --> 00:40:00,950
+ال .. الكمية من ال daily want انا بكون فوتت الدم
+
+518
+00:40:00,950 --> 00:40:06,160
+للمستودع يعني أمنته موجود في المستودعتمام؟ طيب
+
+519
+00:40:06,160 --> 00:40:09,560
+فإذا و بعد هيك بقايا ال stem اللي هي فيها daily
+
+520
+00:40:09,560 --> 00:40:13,880
+one لحالي اللي هي أصلا كلها حجمها واحد بتخلص منه
+
+521
+00:40:13,880 --> 00:40:18,420
+على قطعة قطن فبصير عندي في النهاية أنا ماخدة نص ل
+
+522
+00:40:18,420 --> 00:40:24,800
+11 في واحد اتخلصت منه يعني نص ل 10 يعني واحد تمام
+
+523
+00:40:24,800 --> 00:40:25,440
+إلى
+
+524
+00:40:31,120 --> 00:40:34,940
+عشرين تمام؟ take ال delayotion طبيعي واحد إلى
+
+525
+00:40:34,940 --> 00:40:40,980
+عشرين تمام جديد بالذكر احكيلكم انه هذه ال .. ال
+
+526
+00:40:40,980 --> 00:40:47,140
+bead اللي هنا بعد ما انا اوصل الدم للمستودع هذه ال
+
+527
+00:40:47,140 --> 00:40:50,860
+bead لو انا عملت mixing رجيت ال .. ال by bit طبعا
+
+528
+00:40:50,860 --> 00:40:55,140
+سكرتها ب ..بأصابعي سكرت فوها من هنا و من هنا و
+
+529
+00:40:55,140 --> 00:40:59,700
+عملت mixing تمام هذه البيض راح تتحرك و هي تخلط
+
+530
+00:40:59,700 --> 00:41:04,280
+الدم مع ال diligent و راح تساعد على تكسير خلايا ال
+
+531
+00:41:04,280 --> 00:41:07,860
+RBCs و تنصبغ ال white BCs بالصبغة اللي موجودة في
+
+532
+00:41:07,860 --> 00:41:12,740
+المحلول علشان في النهاية انا احصل على محلول متجانس
+
+533
+00:41:12,740 --> 00:41:19,890
+أملؤه داخل ال chamberطيب أنا عديت في مربعات المربع
+
+534
+00:41:19,890 --> 00:41:24,990
+كل واحد منه كان ستة .. يعني مقسم إلى ستاشر مربع
+
+535
+00:41:24,990 --> 00:41:29,430
+صغير هنا المربعات هذه أنا عديت أربع مربعات كل واحد
+
+536
+00:41:29,430 --> 00:41:33,190
+فيهم ستاشر مربع صغير طب هذا المربع الكبير اللي فيه
+
+537
+00:41:33,190 --> 00:41:37,170
+ستاش ايش أبعاده عبارة عن واحد millimeter في واحد
+
+538
+00:41:37,170 --> 00:41:41,910
+millimeter أبعاده؟تمام؟ يعني طيب فإذا لو أنا بدي
+
+539
+00:41:41,910 --> 00:41:46,210
+أجي أخد حجم المربع الواحد عبارة عن .. طب الحجم هو
+
+540
+00:41:46,210 --> 00:41:49,750
+عبارة عن إيش؟ تلت أبعاد طول في عرض في ارتفاع طيب
+
+541
+00:41:49,750 --> 00:41:53,770
+واحد millimeter طول و عرض واحد millimeter طول طب
+
+542
+00:41:53,770 --> 00:41:58,270
+ارتفاعه جداش كان؟ كان هو اللي هو المسافة أو البعد
+
+543
+00:41:58,270 --> 00:42:04,210
+ما بين ال chamber و ال slide ال cover slip اللي
+
+544
+00:42:04,210 --> 00:42:11,620
+محطوط تمام؟ فإذا ..حجم المربع هيكون واحد من عشرة
+
+545
+00:42:11,620 --> 00:42:17,720
+ملي متر مكعب وبما إن السوائل تاخد حجم القناع اللي
+
+546
+00:42:17,720 --> 00:42:23,260
+بتوضع فيه، تمام؟ إذا حجم السائل اللي أنا قرأته في
+
+547
+00:42:23,260 --> 00:42:28,060
+المربع هذا هو حجم المربع الطول في العرض في
+
+548
+00:42:28,060 --> 00:42:31,540
+الارتفاع في كام مسافة كام ما بين ال chamber هذا و
+
+549
+00:42:31,540 --> 00:42:37,960
+ال cover slip تمام؟طيب، أنا عديت في أربع مربعات،
+
+550
+00:42:37,960 --> 00:42:41,000
+يعني خلّينا نحسب إنه أنا عديت في منطقة واحدة من
+
+551
+00:42:41,000 --> 00:42:45,100
+الأتشاب، في أربع مربعات، هيهم، تمام؟ المنطقة
+
+552
+00:42:45,100 --> 00:42:51,520
+الواحدة من الأتشاب بتكون هيك مسطرة، تمام؟
+
+553
+00:42:51,520 --> 00:42:55,580
+هي الأتشاب، المنطقة الواحدة بتكون هيك مسطرة، زي ما
+
+554
+00:42:55,580 --> 00:43:01,920
+شفنا، و هنا كمان موجود أيضًا تسطيرة أخرىطيب أنا
+
+555
+00:43:01,920 --> 00:43:05,520
+عديت فيه أربع مربعات معناها الحجم اللي حسبته
+
+556
+00:43:05,520 --> 00:43:10,140
+للمربع الواحد بضربه فيه أربعة ففي عندي الحجم لأربع
+
+557
+00:43:10,140 --> 00:43:13,660
+مربعات هو عبارة عن أربعة في الواحد من عشرة
+
+558
+00:43:13,660 --> 00:43:18,380
+millimeter مكعب و هيعطيني فاصلة أربعة من عشرة أو
+
+559
+00:43:18,380 --> 00:43:22,860
+point أربعة millimeter مكعب ال millimeter مكعب هو
+
+560
+00:43:22,860 --> 00:43:25,540
+نفس ال microliter طيب
+
+561
+00:43:31,840 --> 00:43:36,740
+نفترض إنه أنا عدت في أربع مربعات خمسين خلية تمام؟
+
+562
+00:43:36,740 --> 00:43:39,540
+في الأربع مربعات كان مجموع العدد خمسين نفترض
+
+563
+00:43:39,540 --> 00:43:46,020
+افتراض طيب الخمسين كانوا موجودين في حجم فاصلة من
+
+564
+00:43:46,020 --> 00:43:51,560
+أربعة من عشرة يعني ملي متر مكعب طب كام بيكون موجود
+
+565
+00:43:51,560 --> 00:43:57,330
+في الواحد ملي متر مكعب؟ لإنه في النهاية أناوحدة
+
+566
+00:43:57,330 --> 00:44:03,470
+قياس الخلايا اللي هي عبارة عن سل أو اللي هي الوحدة
+
+567
+00:44:03,470 --> 00:44:10,030
+لعد الخلايا سل per micrometer مكعب، تمام؟ per
+
+568
+00:44:10,030 --> 00:44:11,230
+micrometer مكعب
+
+569
+00:44:16,630 --> 00:44:20,150
+مش موجود القص هنا مش عارفة من وين اجيبه ما علينا
+
+570
+00:44:20,150 --> 00:44:24,050
+متر م .. ملي متر مكعب او ممكن تكون اللي هي عبارة
+
+571
+00:44:24,050 --> 00:44:31,690
+عن سل بير microliter تمام بير microliter طيب انا
+
+572
+00:44:31,690 --> 00:44:34,930
+في ال microliter الواحد كام بيكون موجود في ال
+
+573
+00:44:34,930 --> 00:44:38,350
+millimeter مكعب الواحد كام بيكون موجود خلايا هذا
+
+574
+00:44:38,350 --> 00:44:44,660
+اللي بلزمني ففي النهاية انا بعمل زي ضرب تبادليو
+
+575
+00:44:44,660 --> 00:44:49,460
+بطلع عندي الخمسين في واحد على أربعة من عشرة طبعا
+
+576
+00:44:49,460 --> 00:44:53,760
+ال volume correction هو الواحد لأربعة من عشرة
+
+577
+00:44:53,760 --> 00:44:57,100
+فدايما بضربه في ال and في العدد الخلايا اللي بيطلع
+
+578
+00:44:57,100 --> 00:45:01,020
+معايا طبعا و هيك طبعا بضربه كمان أيضا في ال
+
+579
+00:45:01,020 --> 00:45:06,800
+dilution factor في ال dilution factor طبعا و
+
+580
+00:45:06,800 --> 00:45:18,360
+بالنهاية بحصل على ال total طبعا ofWhite PCsتمام؟
+
+581
+00:45:18,360 --> 00:45:22,880
+إذا في النهاية أنا بالزمني أكون عارفة أبعاد المربع
+
+582
+00:45:22,880 --> 00:45:28,020
+عشان أجيب الحجم و .. و أجيب .. و الحجم بالزمني
+
+583
+00:45:28,020 --> 00:45:33,400
+عشان أنا في النهاية بدي بدل ما أحسب أدد الخلايا في
+
+584
+00:45:33,400 --> 00:45:38,360
+point أربعة ملي متر مكعب بدي أحسبه في واحد ملي متر
+
+585
+00:45:38,360 --> 00:45:42,900
+مكعب تمام؟ فب .. ب .. بعمل يعني .. بدرب العدد في
+
+586
+00:45:42,900 --> 00:45:48,270
+مقلوب هذا ال volume تمام؟فهيك بكون جبت ال volume
+
+587
+00:45:48,270 --> 00:45:52,290
+correction طب دلت العينة مخففة فعندي فانا بدي
+
+588
+00:45:52,290 --> 00:45:57,670
+العينة ك .. ك .. ك .. أصل مجدش فيها عدد خلايا فبدي
+
+589
+00:45:57,670 --> 00:46:00,830
+أضرب في ال dilution factor و احنا عرفنا لما دايما
+
+590
+00:46:00,830 --> 00:46:04,430
+لما بدي أجيب الأصل ال original بدرب في ال dilution
+
+591
+00:46:04,430 --> 00:46:09,590
+factorفإذا ال total of white pieces count هيكون هو
+
+592
+00:46:09,590 --> 00:46:12,910
+عدد الخلايا اللي أنا عدتها في كام مربع؟ في أربع
+
+593
+00:46:12,910 --> 00:46:17,090
+مربعات عشان هيك لو أنا جبت يعني يا بعد في جهة
+
+594
+00:46:17,090 --> 00:46:20,790
+واحدة يا إما بعد في الجهتين و بقسم على اتنين لإن
+
+595
+00:46:20,790 --> 00:46:24,050
+لو عدت في الجهتين هيكون في عندي تمان قراءات فأنا
+
+596
+00:46:24,050 --> 00:46:28,330
+بدي لأ أنا بدي فقط أربع قراءات مجموع أربع قراءات
+
+597
+00:46:28,330 --> 00:46:33,800
+تمام؟ مجموع الخلايا في أربع مربعات فقط لا غيرو
+
+598
+00:46:33,800 --> 00:46:36,560
+بدربه في ال volume correction و ايضا في ال
+
+599
+00:46:36,560 --> 00:46:40,120
+dilution factor طبعا هذه التفاصيل ان شاء الله ممكن
+
+600
+00:46:40,120 --> 00:46:48,380
+نوضحها اكتر في المعمل و ننتقل الى ال discussion او
+
+601
+00:46:48,380 --> 00:46:53,500
+الجزئية اللى بتتعلق بصرد .. صرد مصادر ال error و
+
+602
+00:46:53,500 --> 00:46:56,820
+كيف احنا نحلها لو كان في عند الشخص عنده
+
+603
+00:46:56,820 --> 00:47:02,800
+leukocytosis اش يعني leukocytosis؟ يعني زيادة high
+
+604
+00:47:03,520 --> 00:47:08,640
+أو elevated leukocyte count عدد كبير او ارتفاع
+
+605
+00:47:08,640 --> 00:47:13,060
+كبير في عدد خلايا الدم البيضاء معناه اش بده اعمل؟
+
+606
+00:47:13,060 --> 00:47:16,220
+بده اعمل dilution اكبر بدل ما اعمل dilution واحد
+
+607
+00:47:16,220 --> 00:47:20,160
+لعشرين بعمل واحد لمية dilution خارجي في ال tube مش
+
+608
+00:47:20,160 --> 00:47:22,860
+شرط استخدم الثومابايبت لأنها مش هتوفرلي هذا ال
+
+609
+00:47:22,860 --> 00:47:26,380
+dilution طيب
+
+610
+00:47:26,380 --> 00:47:29,760
+لو كان الشخص عنده
+
+611
+00:47:33,780 --> 00:47:39,120
+ليكو سايتو بينيا
+
+612
+00:47:40,620 --> 00:47:45,840
+leukocytopenia اللي هي يعني low like leukocyte
+
+613
+00:47:45,840 --> 00:47:49,920
+count تمام او reduced leukocyte count يعني عدد
+
+614
+00:47:49,920 --> 00:47:55,080
+قليل من ال ال white pieces تمام يعني اقل مثلا من
+
+615
+00:47:55,080 --> 00:48:01,060
+3000 خلية per millimeter فبالتالي انا بدي اقلل ال
+
+616
+00:48:01,060 --> 00:48:03,940
+dilution هنا لو انا عملت dilution 1 ل 20 و هو اصلا
+
+617
+00:48:03,940 --> 00:48:07,560
+عنده نقص في خلايا الدم البيضاء معناه مش هشوف خلايا
+
+618
+00:48:07,560 --> 00:48:11,720
+لإن انا مخففاهافبدى اقلل ال dilution بعمل dilution
+
+619
+00:48:11,720 --> 00:48:17,840
+واحد لعشرة بيكون كافي ممكن انه يعني مثلا بعبي الدم
+
+620
+00:48:17,840 --> 00:48:24,400
+لعندي الواحد و بكمل لالإحداش تمام؟ بتخلص من واحد
+
+621
+00:48:24,400 --> 00:48:28,700
+من خلال القطارات فبيصير ال dilution واحد لعشرة او
+
+622
+00:48:28,700 --> 00:48:33,180
+ممكن اعمله ك dilution خارجي في طيوب و هنعلمكم كيف
+
+623
+00:48:33,180 --> 00:48:34,880
+بنعمل dilution خارجي في طيوب
+
+624
+00:48:39,440 --> 00:48:43,600
+طيب لو كان .. لو أنا كنت عاملة مسحة دم ل .. للمريض
+
+625
+00:48:43,600 --> 00:48:49,260
+و لاجيت عنده في ال blood smear in nucleated RBCs
+
+626
+00:48:49,260 --> 00:48:53,700
+هاي ال nucleated RBCs ممكن ما تتكسر بفعل ال terg
+
+627
+00:48:53,700 --> 00:48:56,600
+solution و تظل موجودة و لما قاعد .. قاعد .. قاعدها
+
+628
+00:48:56,600 --> 00:49:02,420
+على أساس إنها white BCs تمام؟ و .. يعني تعملي
+
+629
+00:49:02,420 --> 00:49:07,050
+errorتزودلي ال .. ال count ال total count طبعا
+
+630
+00:49:07,050 --> 00:49:10,610
+فأنا لازم ساعتها هنا لو لمحت في ال blood smear في
+
+631
+00:49:10,610 --> 00:49:14,430
+ال .. في الفيلم في مسحة الدم خلايا nucleated وكنت
+
+632
+00:49:14,430 --> 00:49:17,790
+عاملة في نفس الوقت white pieces count لازم أروح
+
+633
+00:49:17,790 --> 00:49:21,250
+أطبق قانون اسمه corrected white pieces count طبعا
+
+634
+00:49:21,250 --> 00:49:26,330
+لإن أنا بكون ساعتها أكيد عديت خلايا RPCs على أساس
+
+635
+00:49:26,330 --> 00:49:29,990
+إنها white piecesإيش هي الـ Nucleated RBCs؟ الـ
+
+636
+00:49:29,990 --> 00:49:34,490
+Nucleated RBCs هي عبارة عن خلايا RBCs غير نادجة،
+
+637
+00:49:34,490 --> 00:49:38,570
+تمام؟ الخلايا النادجة هي اللي بتفقد النواة تبعتها،
+
+638
+00:49:38,570 --> 00:49:42,490
+لطالما موجودة النواة معناها هي خلايا غير نادجة
+
+639
+00:49:42,490 --> 00:49:46,270
+طلعت ع ال circulation بفعل ضغط على البون مارو مثلا
+
+640
+00:49:46,270 --> 00:49:50,330
+في نزيف أو أنيميا أو مشكلة معينة أن ال RBCs مش
+
+641
+00:49:50,330 --> 00:49:53,810
+كافية بس بيصير البون مارو يطلع ال RBCs وهي غير
+
+642
+00:49:53,810 --> 00:49:58,310
+نادجةعلى الدم عشان تحلي المشكلة اللي برا تحلي هي
+
+643
+00:49:58,310 --> 00:50:04,530
+مشكلة ال high box تمام حكينا انه هاي ال RBCs ال
+
+644
+00:50:04,530 --> 00:50:09,150
+nucleated RBCs resist lysis ما .. ما بتتكسر بفعل
+
+645
+00:50:09,150 --> 00:50:12,410
+ال diluent فانا لو لمحتها في ال blood film طبعا
+
+646
+00:50:12,410 --> 00:50:15,690
+انا مابشوفها على ال .. ال chamber ممكن اشوفها في
+
+647
+00:50:15,690 --> 00:50:21,710
+مسحة الدمإذا لمحتها في مسحة الدم معناها وكنت عاملة
+
+648
+00:50:21,710 --> 00:50:24,790
+في نفس الوقت white pieces count معناها في عندي خلل
+
+649
+00:50:24,790 --> 00:50:27,890
+أو في عندي زيادة في ال white pieces count سببه هذه
+
+650
+00:50:27,890 --> 00:50:30,750
+ال nucleated RBCs اللي انعدت على أساس أنها white
+
+651
+00:50:30,750 --> 00:50:34,230
+pieces فلازم أطبق هذا القانون اللي هو عبارة عن
+
+652
+00:50:34,230 --> 00:50:37,030
+خلايا ال white pieces اللي .. اللي أنا عديتها
+
+653
+00:50:37,030 --> 00:50:42,450
+وكانت يعني فيها زيادة فيها .. هي uncorrect ضرب 100
+
+654
+00:50:42,960 --> 00:50:48,320
+و على 100 زائد اللي هي ال nucleated RBCs اللي
+
+655
+00:50:48,320 --> 00:50:52,920
+موجودة في .. يعني عدد ال nucleated RBCs اللي
+
+656
+00:50:52,920 --> 00:50:56,780
+موجودة في مسحة الدم تمام؟ و أنا بعمل differential
+
+657
+00:50:56,780 --> 00:51:01,920
+لما أجي أعد خلايا white bases على ال blood smear
+
+658
+00:51:01,920 --> 00:51:06,500
+بشوف .. يعني ب .. بعد ال nucleated RBCs و بستخدمها
+
+659
+00:51:06,500 --> 00:51:10,280
+في تطبيق هذا القانونإيش يعني مسحة الدم و كيف أنا
+
+660
+00:51:10,280 --> 00:51:13,300
+بشوف ال nucleated RBCs و إيش يعني أصلا white
+
+661
+00:51:13,300 --> 00:51:16,840
+pieces differential هذا راح ناخده في معمل blood
+
+662
+00:51:16,840 --> 00:51:21,440
+film أو blood smear تمام؟ هتضح .. هتضح إلكم الصورة
+
+663
+00:51:21,440 --> 00:51:30,970
+أكترطيب sources of error حكينا إن الخلل ممكن يكون
+
+664
+00:51:30,970 --> 00:51:36,770
+موجود فيه سببه failure to have required blood
+
+665
+00:51:36,770 --> 00:51:40,600
+volume يعني أنا ما ملقت الدم للنصفي حال .. في
+
+666
+00:51:40,600 --> 00:51:45,020
+الحالات الطبيعية ماملقته للواحد في حالات انه مثلا
+
+667
+00:51:45,020 --> 00:51:50,260
+عنده leukocytopenia ماعملت dilution كويس في حالات
+
+668
+00:51:50,260 --> 00:51:54,720
+ال leukocytosis مااخدتش الحجم الدم اللازم فإذا انا
+
+669
+00:51:54,720 --> 00:52:01,760
+مااخدتش كمية دم معبرة تمام فبالتالي الفحص التابعي
+
+670
+00:52:01,760 --> 00:52:04,940
+أيضا مش راح يكون معبر و النتائج انتباهتي مش راح
+
+671
+00:52:04,940 --> 00:52:08,610
+تكون دقيقةfailure to mix well إذا أنا ماعملت
+
+672
+00:52:08,610 --> 00:52:14,070
+mixing منيح لل blood مع ال .. ال daily one معناها
+
+673
+00:52:14,070 --> 00:52:18,210
+أنا برضه كمان هنا ممكن أاخد concentrated أو أخد
+
+674
+00:52:18,210 --> 00:52:24,430
+مثلا دم مش مكسرة فيه ال RBCs فبالتالي اتعيق عملية
+
+675
+00:52:24,430 --> 00:52:29,660
+القراءةfailure to discard the first four drops لو
+
+676
+00:52:29,660 --> 00:52:33,560
+أنا ما اتخلصت من الأربع قطرات أو الثلاث قطرات اللي
+
+677
+00:52:33,560 --> 00:52:40,380
+بيكونوا موجودين في أول الجزء من الثومة بعيبة اللي
+
+678
+00:52:40,380 --> 00:52:45,060
+هو الstemمعناها انه انا لما اهجأ املأ ال chamber
+
+679
+00:52:45,060 --> 00:52:50,000
+هاملأه فيهم هدول القطارات يعني هاملأه بترك
+
+680
+00:52:50,000 --> 00:52:53,320
+solution لحال مش راح يكون في عندي عينة كافية على
+
+681
+00:52:53,320 --> 00:52:56,140
+ال chamber فبالتالي هيكون ال white pieces count
+
+682
+00:52:56,140 --> 00:53:01,430
+قليل نتيجة ان انا محطتش عينة كفايةfailure to .. و
+
+683
+00:53:01,430 --> 00:53:04,850
+ايضا كمان ال delution تبعي هيكون فيه خلال failure
+
+684
+00:53:04,850 --> 00:53:07,710
+to barely charge the counting chamber لو انا
+
+685
+00:53:07,710 --> 00:53:11,910
+مملقتش ال chamber بطريقة صحيحة ايضا كمان راح يكون
+
+686
+00:53:11,910 --> 00:53:15,730
+في عندي خلال لإن زي ما انتوا شايفين الحسابات تبعتي
+
+687
+00:53:15,730 --> 00:53:19,930
+بلزمني الحجم اللي كان .. الحجم ال blood اللي هو
+
+688
+00:53:19,930 --> 00:53:25,790
+حجم المربعات اللي كان يملقها ال blood تمام؟ و
+
+689
+00:53:25,790 --> 00:53:30,070
+علشان احصل على volume correctionوبلزمني أيضًا
+
+690
+00:53:30,070 --> 00:53:33,510
+delusion factor عشان أحصل على اللي .. اللي هو
+
+691
+00:53:33,510 --> 00:53:39,240
+الصيغة كاملةfailure to preparely chart the
+
+692
+00:53:39,240 --> 00:53:41,960
+counting chamber إذا أنا ما ملاقت ال chamber بشكل
+
+693
+00:53:41,960 --> 00:53:46,060
+منيح معناها القانون اللي أنا بتبقه خطأ و مش معبر
+
+694
+00:53:46,060 --> 00:53:50,380
+فبالتالي نتائجي فيها خطأ أيضا كمان في عندي مشاكل
+
+695
+00:53:50,380 --> 00:53:55,060
+تانية ل ال error أقل يعني شيوعا ولكن ممكن تكون
+
+696
+00:53:55,060 --> 00:53:58,760
+موجودة ال by بتكون فيها مشكلة مثلا أو ال chamber
+
+697
+00:53:58,760 --> 00:54:04,070
+في مشكلة يكون inaccurateتستيرته خطأ، أبعاده،
+
+698
+00:54:04,070 --> 00:54:09,070
+البيبط فيها مشكلة، انكسرات، سبددات أو إشي، moist
+
+699
+00:54:09,070 --> 00:54:12,850
+or unclean بيبط إذا كانت ال .. البيبط من جوا فيها
+
+700
+00:54:12,850 --> 00:54:17,390
+مبللة أو فيها سائل أو مش نظيفة، أيضا كمان هذا ..
+
+701
+00:54:17,390 --> 00:54:22,070
+يعني إشي راح يشغل حيز و بالتالي راح يأثر على الحجم
+
+702
+00:54:22,070 --> 00:54:25,470
+و يأثر على ال dilution تبعيexcessive pressure in
+
+703
+00:54:25,470 --> 00:54:29,690
+finger لو أنا أخدت عينة من ال .. ال finger من ال
+
+704
+00:54:29,690 --> 00:54:33,490
+skin puncture وعملت pressure يعني ضغط جامد فمعناها
+
+705
+00:54:33,490 --> 00:54:37,170
+أنا بكون عم بطلع عصارة خلاياتي شجوس فبالتالي ال
+
+706
+00:54:37,170 --> 00:54:40,470
+blood هيكون diluted فبالتالي مش هيكون أيضا معبر
+
+707
+00:54:41,390 --> 00:54:46,050
+هحصل على reduced او low white basis count too
+
+708
+00:54:46,050 --> 00:54:48,930
+little or too much diluting fluid لو انا حطيت
+
+709
+00:54:48,930 --> 00:54:52,870
+diluting fluid بكميات كبيرة او بكميات قليلة هدها
+
+710
+00:54:52,870 --> 00:54:56,930
+يأثر على ال dilution slowness in manipulation وهي
+
+711
+00:54:56,930 --> 00:55:04,190
+نقطة مهمة انه نسرع او انه نكون هيك يعنيكيف دي
+
+712
+00:55:04,190 --> 00:55:09,650
+أحكيها ناشطين يعني انه مش بطق في الخطوات و روح
+
+713
+00:55:09,650 --> 00:55:13,470
+نفارطة لأ لإنه فعليا مثلا لو انت بدك تاخدي عينة من
+
+714
+00:55:13,470 --> 00:55:16,510
+ال skin puncture عشان تعبي فيها ثمابيبت لو انت ما
+
+715
+00:55:16,510 --> 00:55:20,330
+أسرعتي و حطيه و على طول ملقتي ال blood للنص و وراه
+
+716
+00:55:20,330 --> 00:55:24,030
+على طول مباشرة حطيت daily one الدم رح يتجلط لإن
+
+717
+00:55:24,030 --> 00:55:27,570
+انت عم تاخديه مباشرة من ال skin puncture يعني
+
+718
+00:55:27,570 --> 00:55:31,590
+مافيش anticoagulant في الموضوع فممكن يتجلط و يسدد
+
+719
+00:55:31,590 --> 00:55:35,600
+ال themabibetلو كان في ال .. في ال thermo pipet
+
+720
+00:55:35,600 --> 00:55:38,760
+air bubbles برضه كمان معناها في مشكلة فلذلك لازم
+
+721
+00:55:38,760 --> 00:55:43,440
+انا اتحكم بنفسي منيح و انا عم باخد ال volumes و
+
+722
+00:55:43,440 --> 00:55:49,580
+ايضا كمان لما افوت ال pipet مثلا في solution
+
+723
+00:55:49,580 --> 00:55:53,380
+افوتها بشكل عمودي يعني في trickات ريحت تعلموها
+
+724
+00:55:53,380 --> 00:55:59,100
+بالشغل العملي كيف انت تمنعي حدوث ال air bubbles او
+
+725
+00:55:59,100 --> 00:56:02,750
+يعني ظهور ال air bubbles في الشغلو برضه كمان ال
+
+726
+00:56:02,750 --> 00:56:05,590
+air bubble في ال counting chamber هتأثر على الحجم
+
+727
+00:56:05,590 --> 00:56:10,930
+اللي أنا عم بعده فبالتالي ممكن تعطيني error إذا
+
+728
+00:56:10,930 --> 00:56:14,750
+كان ال .. ال .. ال chamber مش نظيف مثلا أو في
+
+729
+00:56:14,750 --> 00:56:20,110
+المحلول تبعي مش نظيف مثلا في yeast أو fungi أو فطر
+
+730
+00:56:20,110 --> 00:56:23,900
+معين عفنفي المحلول أو حتى كمان ممكن يكون على ال
+
+731
+00:56:23,900 --> 00:56:28,520
+chamber ما .. عليه غبار أو ما مسح كويس فبالتالي
+
+732
+00:56:28,520 --> 00:56:33,740
+هذا هيأثر لإن هتعيق عملية العد هذه الغبار و ال ..
+
+733
+00:56:33,740 --> 00:56:39,840
+ال contaminationPresence of many nucleated red
+
+734
+00:56:39,840 --> 00:56:43,280
+cells causing a high white cell count إذا كان في
+
+735
+00:56:43,280 --> 00:56:48,580
+عندي عديد من الخلايا ال .. ال red cell نيوكلياتد
+
+736
+00:56:48,580 --> 00:56:52,920
+تمام معناه هذا هيعطيني error فلذلك أو إذا كان أنا
+
+737
+00:56:52,920 --> 00:56:57,360
+بشتغل white bc count مع blood film مع بعض و لمحت
+
+738
+00:56:57,360 --> 00:57:01,820
+في ال blood film نيوكلياتد red cell على طول لازم
+
+739
+00:57:01,820 --> 00:57:05,700
+أروح أطبق قانون ال corrected white bc count
+
+740
+00:57:07,130 --> 00:57:10,110
+mistakes in counting or calculation إذا كان أنا
+
+741
+00:57:10,110 --> 00:57:14,950
+طبقت القانون غلط مثلا يعني هنا حطيت ال and تكون
+
+742
+00:57:14,950 --> 00:57:20,010
+اللي هي مجموع عدد الخلايا في ال 8 مربعات مثلا أو
+
+743
+00:57:20,010 --> 00:57:23,710
+في 3 مربعات غلط إذا أنا عديت في 8 يعني عديت في
+
+744
+00:57:23,710 --> 00:57:30,730
+الجهتين فوق و تحت لازم أقسم ع 2 لازم هذا يكون ال
+
+745
+00:57:30,730 --> 00:57:33,870
+number of cells هو مجموع الخلايا في الأربع مربعات
+
+746
+00:57:33,870 --> 00:57:34,730
+تمام؟
+
+747
+00:57:39,680 --> 00:57:43,900
+أو حتى في الحسابات يعني مثلا ما جبت ال correction
+
+748
+00:57:43,900 --> 00:57:50,920
+volume أو ال volume correction صح ما جبت ال
+
+749
+00:57:50,920 --> 00:57:57,000
+dilution factor بشكل صح دي برضه كمان رح يأثر بهيك
+
+750
+00:57:57,000 --> 00:58:00,460
+بنكون وصلنا لنهاية محاضرتنا لليوم أتمنى أنها تكون
+
+751
+00:58:00,460 --> 00:58:04,180
+واضحة ومش صعبة عليكم يعطيكم ألف عافية
+
diff --git a/PL9fwy3NUQKwY9jl8xw-bWkVZkWcGGyspd/DG-2O71i3Fw_raw.json b/PL9fwy3NUQKwY9jl8xw-bWkVZkWcGGyspd/DG-2O71i3Fw_raw.json
new file mode 100644
index 0000000000000000000000000000000000000000..2d2f9cee6e3fa4ccb8376bcb526144ecc81c18d8
--- /dev/null
+++ b/PL9fwy3NUQKwY9jl8xw-bWkVZkWcGGyspd/DG-2O71i3Fw_raw.json
@@ -0,0 +1 @@
+{"segments": [{"id": 1, "seek": 3809, "start": 9.95, "end": 38.09, "text": "السلام عليكم ورحمة الله وبركاته طالبات العزيزات اليوم ان شاء الله راح نشرح محاضرة بعنوان white blood cell count في نهاية هذه المحاضرة راح نكون بنعرف كيف احنا بنعد خلايا الدم البيضاء بطريقة manual بطريقة يدوية وكيف بنحصل على عدد خلايا الدم البيضاء as a total اذا white blood cell count معناها عد خلايا الدم البيضاء", "tokens": [6027, 3794, 37440, 25894, 24793, 4032, 2288, 35571, 3660, 21984, 4032, 26890, 4117, 9307, 3224, 23032, 6027, 3555, 9307, 18863, 11622, 1829, 11622, 9307, 45595, 20498, 16472, 13412, 16606, 21984, 12602, 39319, 8717, 46309, 5016, 3714, 5016, 46958, 25720, 45030, 1863, 2407, 7649, 2418, 3390, 2815, 1207, 8978, 8717, 11296, 10632, 29538, 9673, 5016, 46958, 25720, 12602, 39319, 8717, 30544, 44945, 3615, 28480, 9122, 33911, 1975, 5016, 8315, 44945, 22488, 16490, 15040, 25528, 32748, 2304, 2423, 21292, 11242, 16606, 4724, 9566, 16572, 28671, 9688, 4724, 9566, 16572, 28671, 7251, 3215, 2407, 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"end": 704.24, "text": "باستخدام شريحة معينة مخصصة للعد انا بحط السائل او المحلول اللي انا حضرته اللي هو عبارة عن الدم ال diligent بحطه على هذه الشريحة و ببدأ اعد على ال microscope تمام؟ هذه هي الفكرة ببساطة خلينا نتعمق فيها اكتر و نشوف ايش تفاصيلها العلمية", "tokens": [3555, 995, 14851, 9778, 3215, 10943, 13412, 16572, 5016, 3660, 20449, 9957, 3660, 3714, 9778, 9381, 9381, 3660, 24976, 22488, 1975, 8315, 4724, 5016, 9566, 21136, 16373, 1211, 1975, 2407, 9673, 5016, 1211, 12610, 13672, 1829, 1975, 8315, 11331, 11242, 43500, 3224, 13672, 1829, 31439, 6225, 3555, 9640, 3660, 18871, 32748, 2304, 2423, 50251, 4724, 5016, 9566, 3224, 15844, 29538, 25124, 16572, 5016, 3660, 4032, 4724, 44510, 10721, 1975, 22488, 15844, 2423, 4532, 4417, 1114, 46811, 10943, 22807, 29538, 39896, 27188, 4117, 25720, 4724, 3555, 3794, 41193, 3660, 16490, 1211, 9957, 995, 8717, 2655, 25957, 4587, 8978, 11296, 1975, 4117, 2655, 2288, 4032, 8717, 8592, 38688, 1975, 1829, 8592, 6055, 5172, 33546, 26895, 11296, 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754.77, "text": "فى tube editor لإنه احنا لما بنسحب عين تدام فى ال editor انا بحكيلكم اعمله good mixing تمام بنعمل good mixing و بعد هيك طبعا انا حكينا انه هذا ال blood ممكن انا استخدمه عشان اعمله dilution مع diluent", "tokens": [5172, 7578, 9917, 8129, 284, 5296, 28814, 1863, 3224, 1975, 5016, 8315, 5296, 15042, 44945, 3794, 5016, 3555, 6225, 9957, 6055, 3215, 10943, 6156, 7578, 2423, 8129, 284, 1975, 8315, 4724, 5016, 4117, 1829, 23275, 2304, 1975, 25957, 43761, 665, 11983, 46811, 10943, 44945, 25957, 1211, 665, 11983, 4032, 39182, 39896, 4117, 23032, 3555, 3615, 995, 1975, 8315, 11331, 4117, 1829, 8315, 16472, 3224, 23758, 2423, 3390, 3714, 43020, 1975, 8315, 44713, 9778, 40448, 3224, 6225, 8592, 7649, 1975, 25957, 43761, 11504, 1448, 20449, 11504, 19226], "avg_logprob": -0.2139008572731895, "compression_ratio": 1.625668449197861, "no_speech_prob": 1.2874603271484375e-05, "words": [{"start": 734.85, "end": 734.95, "word": "فى", "probability": 0.6019287109375}, {"start": 734.95, 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"probability": 0.9879557291666666}, {"start": 3443.61, "end": 3443.71, "word": " في", "probability": 0.984375}, {"start": 3443.71, "end": 3444.31, "word": " الجهتين", "probability": 0.9759521484375}, {"start": 3444.31, "end": 3444.67, "word": " فوق", "probability": 0.86181640625}, {"start": 3444.67, "end": 3445.77, "word": " و", "probability": 0.37060546875}, {"start": 3445.77, "end": 3446.15, "word": " تحت", "probability": 0.92822265625}, {"start": 3446.15, "end": 3446.89, "word": " لازم", "probability": 0.9716796875}, {"start": 3446.89, "end": 3447.57, "word": " أقسم", "probability": 0.9514973958333334}, {"start": 3447.57, "end": 3447.75, "word": " ع", "probability": 0.6513671875}, {"start": 3447.75, "end": 3448.05, "word": " 2", "probability": 0.88818359375}, {"start": 3448.05, "end": 3449.07, "word": " لازم", "probability": 0.98193359375}, {"start": 3449.07, "end": 3449.99, "word": " هذا", "probability": 0.8955078125}, {"start": 3449.99, "end": 3450.51, "word": " يكون", "probability": 0.98876953125}, {"start": 3450.51, "end": 3450.73, "word": " ال", "probability": 0.75927734375}, {"start": 3450.73, "end": 3451.03, "word": " number", "probability": 0.98779296875}, {"start": 3451.03, "end": 3451.23, "word": " of", "probability": 0.98583984375}, {"start": 3451.23, "end": 3451.47, "word": " cells", "probability": 0.904296875}, {"start": 3451.47, "end": 3451.65, "word": " هو", "probability": 0.9716796875}, {"start": 3451.65, "end": 3452.09, "word": " مجموع", "probability": 0.982421875}, {"start": 3452.09, "end": 3452.51, "word": " الخلايا", "probability": 0.9820963541666666}, {"start": 3452.51, "end": 3452.65, "word": " في", "probability": 0.97998046875}, {"start": 3452.65, "end": 3453.03, "word": " الأربع", "probability": 0.7923177083333334}, {"start": 3453.03, "end": 3453.87, "word": " مربعات", "probability": 0.9952392578125}, {"start": 3453.87, "end": 3454.73, "word": " تمام؟", "probability": 0.8543294270833334}], "temperature": 1.0}, {"id": 153, "seek": 348418, "start": 3459.68, "end": 3484.18, "text": "أو حتى في الحسابات يعني مثلا ما جبت ال correction volume أو ال volume correction صح ما جبت ال dilution factor بشكل صح دي برضه كمان رح يأثر بهيك بنكون وصلنا لنهاية محاضرتنا لليوم أتمنى أنها تكون واضحة ومش صعبة عليكم يعطيكم ألف عافية", "tokens": [10721, 2407, 11331, 49975, 8978, 21542, 3794, 16758, 9307, 37495, 22653, 50113, 15040, 19446, 10874, 3555, 2655, 2423, 19984, 5523, 34051, 2423, 5523, 19984, 20328, 5016, 19446, 10874, 3555, 2655, 2423, 11504, 1448, 5952, 4724, 8592, 28820, 20328, 5016, 11778, 1829, 4724, 43042, 3224, 9122, 2304, 7649, 12602, 5016, 7251, 10721, 49115, 39627, 1829, 4117, 44945, 30544, 4032, 36520, 8315, 5296, 1863, 11296, 10632, 3714, 5016, 46958, 43500, 8315, 5296, 20292, 20498, 5551, 39237, 1863, 7578, 14739, 11296, 6055, 30544, 4032, 46958, 5016, 3660, 4032, 2304, 8592, 20328, 3615, 49401, 25894, 24793, 37495, 9566, 1829, 24793, 5551, 46538, 6225, 31845, 10632], "avg_logprob": -0.12423407125706766, "compression_ratio": 1.6801801801801801, "no_speech_prob": 9.357929229736328e-06, "words": [{"start": 3459.68, "end": 3459.96, "word": "أو", "probability": 0.7919921875}, {"start": 3459.96, "end": 3460.18, "word": " حتى", "probability": 0.962890625}, {"start": 3460.18, "end": 3460.32, "word": " في", "probability": 0.8984375}, {"start": 3460.32, "end": 3460.86, "word": " الحسابات", "probability": 0.88916015625}, {"start": 3460.86, "end": 3461.08, "word": " يعني", "probability": 0.8076171875}, {"start": 3461.08, "end": 3461.52, "word": " مثلا", "probability": 0.94775390625}, {"start": 3461.52, "end": 3463.12, "word": " ما", "probability": 0.74951171875}, {"start": 3463.12, "end": 3463.44, "word": " جبت", "probability": 0.88427734375}, {"start": 3463.44, "end": 3463.56, "word": " ال", "probability": 0.96484375}, {"start": 3463.56, "end": 3463.9, "word": " correction", "probability": 0.95361328125}, {"start": 3463.9, "end": 3464.34, "word": " volume", "probability": 0.9619140625}, {"start": 3464.34, "end": 3465.7, "word": " أو", "probability": 0.5126953125}, {"start": 3465.7, "end": 3465.82, "word": " ال", "probability": 0.94384765625}, {"start": 3465.82, "end": 3466.04, "word": " volume", "probability": 0.9794921875}, {"start": 3466.04, "end": 3466.58, "word": " correction", "probability": 0.96044921875}, {"start": 3466.58, "end": 3467.12, "word": " صح", "probability": 0.99609375}, {"start": 3467.12, "end": 3469.04, "word": " ما", "probability": 0.78515625}, {"start": 3469.04, "end": 3469.66, "word": " جبت", "probability": 0.9806315104166666}, {"start": 3469.66, "end": 3470.92, "word": " ال", "probability": 0.96337890625}, {"start": 3470.92, "end": 3471.28, "word": " dilution", "probability": 0.828369140625}, {"start": 3471.28, "end": 3471.76, "word": " factor", "probability": 0.90869140625}, {"start": 3471.76, "end": 3472.18, "word": " بشكل", "probability": 0.9954427083333334}, {"start": 3472.18, "end": 3472.58, "word": " صح", "probability": 0.9970703125}, {"start": 3472.58, "end": 3472.84, "word": " دي", "probability": 0.4931640625}, {"start": 3472.84, "end": 3473.1, "word": " برضه", "probability": 0.9501953125}, {"start": 3473.1, "end": 3473.52, "word": " كمان", "probability": 0.9752604166666666}, {"start": 3473.52, "end": 3474.68, "word": " رح", "probability": 0.752685546875}, {"start": 3474.68, "end": 3475.16, "word": " يأثر", "probability": 0.9034830729166666}, {"start": 3475.16, "end": 3477.0, "word": " بهيك", "probability": 0.7316080729166666}, {"start": 3477.0, "end": 3477.64, "word": " بنكون", "probability": 0.912841796875}, {"start": 3477.64, "end": 3478.02, "word": " وصلنا", "probability": 0.9796549479166666}, {"start": 3478.02, "end": 3478.48, "word": " لنهاية", "probability": 0.9669189453125}, {"start": 3478.48, "end": 3479.1, "word": " محاضرتنا", "probability": 0.939453125}, {"start": 3479.1, "end": 3479.5, "word": " لليوم", "probability": 0.9274088541666666}, {"start": 3479.5, "end": 3479.92, "word": " أتمنى", "probability": 0.8160400390625}, {"start": 3479.92, "end": 3480.14, "word": " أنها", "probability": 0.75341796875}, {"start": 3480.14, "end": 3480.46, "word": " تكون", "probability": 0.98974609375}, {"start": 3480.46, "end": 3481.1, "word": " واضحة", "probability": 0.982177734375}, {"start": 3481.1, "end": 3482.3, "word": " ومش", "probability": 0.8541666666666666}, {"start": 3482.3, "end": 3482.56, "word": " صعبة", "probability": 0.9827473958333334}, {"start": 3482.56, "end": 3483.06, "word": " عليكم", "probability": 0.963623046875}, {"start": 3483.06, "end": 3483.54, "word": " يعطيكم", "probability": 0.895263671875}, {"start": 3483.54, "end": 3483.78, "word": " ألف", "probability": 0.791748046875}, {"start": 3483.78, "end": 3484.18, "word": " عافية", "probability": 0.9759114583333334}], "temperature": 1.0}], "language": "ar", "language_probability": 1.0, "duration": 3488.75175, "duration_after_vad": 3381.9937499999946}
\ No newline at end of file
diff --git a/PL9fwy3NUQKwY9jl8xw-bWkVZkWcGGyspd/ELhdSBMOyuo.srt b/PL9fwy3NUQKwY9jl8xw-bWkVZkWcGGyspd/ELhdSBMOyuo.srt
new file mode 100644
index 0000000000000000000000000000000000000000..dbc93615d92cd241d32340982de781dee9c9b24a
--- /dev/null
+++ b/PL9fwy3NUQKwY9jl8xw-bWkVZkWcGGyspd/ELhdSBMOyuo.srt
@@ -0,0 +1,2872 @@
+1
+00:00:11,190 --> 00:00:14,530
+السلام عليكم ورحمة الله وبركاته طالبات العزيزات
+
+2
+00:00:14,530 --> 00:00:18,470
+اليوم إن شاء الله راح نتحدث عن blood smear
+
+3
+00:00:18,470 --> 00:00:22,770
+examination and preparation، تمام؟ هو blood smear
+
+4
+00:00:22,770 --> 00:00:27,750
+preparation and examination، المفروض اليوم نتعلم شو
+
+5
+00:00:27,750 --> 00:00:31,970
+يعني مسحة الدم؟ كيف أنا بحضر هاي المسحة؟ وكيف ممكن
+
+6
+00:00:31,970 --> 00:00:35,780
+أنا أصبغها وأشوفها تحت ال microscope؟ لحتى أستكشف
+
+7
+00:00:35,780 --> 00:00:41,800
+خلايا الدم في البداية، ال blood smear هو عبارة عن
+
+8
+00:00:41,800 --> 00:00:46,940
+مسحة أو فرد للدم على الشريحة، تمام؟ فرد لعينة الدم
+
+9
+00:00:46,940 --> 00:00:50,940
+على الشريحة بحيث أني أنا بإمكاني أن أنا أثبت هذه
+
+10
+00:00:50,940 --> 00:00:55,560
+العينة على الشريحة، ومن ثم أصبغها، وبعد هيك أشوف
+
+11
+00:00:55,560 --> 00:01:00,180
+أتعرف على أشكال خلايا الدم، ليش طيب أنا بدي أتعرف
+
+12
+00:01:00,180 --> 00:01:04,620
+على ال morphology of RBCs أو..أو ال morphology
+
+13
+00:01:04,620 --> 00:01:10,480
+of white BCs؟ لأنه أشكال خلايا الدم سواء كانت white
+
+14
+00:01:10,480 --> 00:01:16,580
+BCs أو RBCs بتكون ذات دلالة أو بتساعدنا في ال
+
+15
+00:01:16,580 --> 00:01:23,210
+diagnosis، في تشخيص بعض الأمراض، تمام؟ مثلا إذا كانت
+
+16
+00:01:23,210 --> 00:01:27,410
+..إذا كان الشخص عنده anemia، فأنا بلاحظ إن ال RBC
+
+17
+00:01:27,410 --> 00:01:31,670
+بتكون فاضية أو ما فيش فيها لون أحمر كثير، تمام؟ إذا
+
+18
+00:01:31,670 --> 00:01:36,010
+كان مثلا في عندي white BC كثيرة، أو ال white BC هذه
+
+19
+00:01:36,010 --> 00:01:39,610
+immature، معناها بأشك أن الشخص ممكن يكون مصاب
+
+20
+00:01:39,610 --> 00:01:46,410
+ب leukemia، حتى يعني زيادة نوع واحد من الخلايا هذا
+
+21
+00:01:46,410 --> 00:01:53,470
+أيضا بيرتبط ب .. بتشخيص معين، إذا أنا لازم أتعرف على
+
+22
+00:01:53,470 --> 00:01:57,810
+أشكال الخلايا هذه، وأتعرف ممكن كمان إن أنا أعد أو
+
+23
+00:01:57,810 --> 00:02:03,250
+أعمل differential لها لحتى أتمكن من المساعدة في
+
+24
+00:02:03,250 --> 00:02:08,410
+التشخيص، تمام، ال blood film هذا دائما بيكون مرافق
+
+25
+00:02:08,410 --> 00:02:11,630
+لل CBC، يعني إذا أنا عملت CBC أو عملت مثلا ال
+
+26
+00:02:11,630 --> 00:02:17,090
+hemoglobin test، عملت hematocrit، حسابة ال MCV و ال
+
+27
+00:02:17,090 --> 00:02:26,370
+..ال .. قانون الثلاثة مثلا، الأشياء هذه، فطبعا لازم
+
+28
+00:02:26,370 --> 00:02:31,790
+معها أكون عملت برضه كمان blood film، there are
+
+29
+00:02:31,790 --> 00:02:35,290
+three types of blood smear، طبعا blood smear أو
+
+30
+00:02:35,290 --> 00:02:39,290
+blood film نفس المعنى، اللي هي مسحة الدم، في عندي
+
+31
+00:02:39,290 --> 00:02:43,670
+ثلاث أنواع منها، مسحة تجرع على ال cover glass أو اللي
+
+32
+00:02:43,670 --> 00:02:47,650
+هو ال .. ال .. ال cover slip اللي أنتو كنتوا
+
+33
+00:02:47,650 --> 00:02:51,810
+تستخدموه لحتى تغطوا ال chamber، ممكن أنا أفرد عليه
+
+34
+00:02:51,810 --> 00:02:56,730
+الدم، فبسميه cover glass smear، ممكن أنا بسم .. أعمل
+
+35
+00:02:56,730 --> 00:03:02,490
+wedge smear باستخدام شريحتين زجاجيات، طبعا، فبنسمي
+
+36
+00:03:02,490 --> 00:03:07,770
+هذا ال .. ال .. السمير wedge smear طبعا، أو ال sponge
+
+37
+00:03:07,770 --> 00:03:13,470
+smear باستخدام الأجهزة، تمام؟ يعني هو electronic
+
+38
+00:03:13,470 --> 00:03:18,730
+أو تقني، تقني، تمام؟ آلي، إذا في عندي ثلاث أنواع من
+
+39
+00:03:18,730 --> 00:03:21,670
+ال blood films أو ال blood smears، احنا اليوم راح
+
+40
+00:03:21,670 --> 00:03:25,850
+نتحدث و أنتو راح تتعلموا بالمختبر ال wedge smear،
+
+41
+00:03:25,850 --> 00:03:29,930
+تمام؟ اللي هو باستخدام two slides، باستخدام two
+
+42
+00:03:29,930 --> 00:03:34,840
+slides، طبعا في عندي أنواع أخرى من ال blood smears
+
+43
+00:03:34,840 --> 00:03:40,020
+أو ال blood film، بيتم تحضيرهم for specific
+
+44
+00:03:40,020 --> 00:03:46,240
+purposes، لأغراض خاصة، مثلا buffy coat smear، يعني أنه
+
+45
+00:03:46,240 --> 00:03:51,340
+أنا أفصل الدم باستخدام ال centrifuge، وآخذ عينة من
+
+46
+00:03:51,340 --> 00:03:54,680
+منطقة ال buffy coat، تمام؟ علشان أنا أفردها على ال
+
+47
+00:03:54,680 --> 00:04:02,640
+slide، وأشوف ال white cells، أشكالها وعدادها، و
+
+48
+00:04:02,640 --> 00:04:06,700
+هذه تجرى للناس اللي عندهم ال white cells أقل من
+
+49
+00:04:06,700 --> 00:04:12,300
+ألف، تمام؟ يعني عندهم leukocytopenia، يعني leukocytes
+
+50
+00:04:12,300 --> 00:04:18,060
+قليلة، تمام؟ طيب في نوع آخر من ال blood films أو ال
+
+51
+00:04:18,060 --> 00:04:22,180
+blood smears، بنسميه thick blood smears، يعني أنه
+
+52
+00:04:22,180 --> 00:04:28,760
+أنا أحضر blood film سميك، تمام؟ أحضر blood film
+
+53
+00:04:28,760 --> 00:04:33,580
+سميك، وهذا بيستخدم لتشخيص ال blood parasites، لتشخيص
+
+54
+00:04:33,580 --> 00:04:38,500
+الطفليات اللي بتعيش في الدم وبتسبب أمراض الدم زي
+
+55
+00:04:38,500 --> 00:04:44,230
+ال malaria مثلا، طيب فإذا أنا بحضر لحتى مثلا أشخص
+
+56
+00:04:44,230 --> 00:04:47,570
+malaria، لازم يكون في عندي thick blood film، إيش
+
+57
+00:04:47,570 --> 00:04:50,330
+يعني thick blood film؟ يعني أنا بستخدم كمية كبيرة
+
+58
+00:04:50,330 --> 00:04:53,950
+من الدم وبفرضها على الشريحة، على .. حتى تعطيني سمك
+
+59
+00:04:53,950 --> 00:04:59,840
+..تعطي سماكة للفيلم، طيب إيش نوع العينة اللي عندي؟
+
+60
+00:04:59,840 --> 00:05:03,020
+لازم تكون العينة EDTA anticoagulant، ممكن أنا
+
+61
+00:05:03,020 --> 00:05:07,340
+أستخدم أنواع أخرى من ال .. ال anticoagulant، ولكن
+
+62
+00:05:07,340 --> 00:05:11,100
+ال most common، اللي أكثر شيوعا وأكثر استخداما هو
+
+63
+00:05:11,100 --> 00:05:15,640
+ال EDTA، طبعا لازم تكون العينة خلال ساعتين أو
+
+64
+00:05:15,640 --> 00:05:20,920
+ثلاث من جمعها، أنا أجري ال blood film أو أحضر ال
+
+65
+00:05:20,920 --> 00:05:26,640
+blood film ليش؟ لأنه إذا طولت العينة ممكن يحدث
+
+66
+00:05:26,640 --> 00:05:32,880
+change على ال RBCs morphology، مثلا يعني أنه تتغير
+
+67
+00:05:32,880 --> 00:05:37,360
+ال RBCs، تبدأ خلايا تموت، تتكسر، ال white BCs ممكن
+
+68
+00:05:37,360 --> 00:05:41,580
+تتغير، فبالتالي إذا أنا لازم أستخدم العينة تكون
+
+69
+00:05:41,580 --> 00:05:45,400
+fresh، أو خلال ساعتين لثلاث من سحبها، أنا أكون
+
+70
+00:05:45,400 --> 00:05:50,150
+حضرت ال blood film، طيب في عوامل أخرى أيضا تؤثر على
+
+71
+00:05:50,150 --> 00:05:54,090
+شكل ال RBCs، ما يتغيرش RBCs morphology، ممكن
+
+72
+00:05:54,090 --> 00:05:57,150
+تخليها speculated أو granulated، يعني shrinkage
+
+73
+00:05:57,150 --> 00:06:00,950
+يصير لها shrinkage، تمام؟ إيش اللي ممكن يسبب لي
+
+74
+00:06:00,950 --> 00:06:04,510
+ال shrinkage؟ أكيد ال excessive amount of anticoagulant،
+
+75
+00:06:04,510 --> 00:06:08,710
+يعني إذا أنا مثلا ما عبيّت دم في أنبوب ال EDTA عند
+
+76
+00:06:08,710 --> 00:06:12,610
+العلامة، معناها ال EDTA زيادة حتكون بالنسبة لل
+
+77
+00:06:12,610 --> 00:06:17,890
+blood، في عندي excessive amount of anticoagulant،
+
+78
+00:06:17,890 --> 00:06:21,130
+إذا ال RBCs راح يصير .. يصير لها shrinkage، راح ي
+
+79
+00:06:21,130 --> 00:06:25,790
+.. ال shrinkage يعني راح تبرز من الخلية شيء ما
+
+80
+00:06:25,790 --> 00:06:30,750
+يشبه ال .. ال .. الأشواك، تمام؟ شيء ما يشبه
+
+81
+00:06:30,750 --> 00:06:36,450
+الأشواك، all the blood longstanding، تمام؟ all the
+
+82
+00:06:36,450 --> 00:06:41,470
+blood longstanding، يعني عينة قديمة أو طولت
+
+83
+00:06:41,470 --> 00:06:45,330
+أيضا بتأثر على ال RBCs morphology، واحنا كنا عم
+
+84
+00:06:45,330 --> 00:06:50,790
+نحكي أن ال RBCs أو ال blood بشكل عام، إذا طولت
+
+85
+00:06:50,790 --> 00:06:55,270
+العينة من ساعتين لثلاث أو أكثر، يعني تجاوزت
+
+86
+00:06:55,270 --> 00:07:01,180
+ساعتين لثلاث، فهذا معناه أن ال RBCs رح يبدأ يتغير
+
+87
+00:07:01,180 --> 00:07:05,280
+شكلها، ال warm environment، ال warm environment
+
+88
+00:07:05,280 --> 00:07:10,040
+هذه بتؤدي إلى أني أنا لما أجيب .. يعني جو حار أو بيئة
+
+89
+00:07:10,040 --> 00:07:14,880
+دافئة، حتؤدي إلى أني وأنا بفرد الدم على الشريحة
+
+90
+00:07:14,880 --> 00:07:21,710
+ممكن يتبخر سائل من ال plasma، تمام؟ هذا راح يؤدي إلى
+
+91
+00:07:21,710 --> 00:07:26,790
+جفاف ال blood بسرعة، أو اللي هي زيادة تركيزه أو
+
+92
+00:07:26,790 --> 00:07:33,370
+جفافه كثير، فبالتالي هيأثر على ال .. ال .. ال RBCs
+
+93
+00:07:33,370 --> 00:07:36,470
+morphology، ويمكن كنتوا تشوفوا أنتو أثناء عد ال
+
+94
+00:07:36,470 --> 00:07:41,990
+RBCs على ال chamber، لما كنتوا تطولوا بالعد كان يجف
+
+95
+00:07:41,990 --> 00:07:46,550
+ال saline، وال RBCs تلاحظوا أنها هربت إلى المنتصف
+
+96
+00:07:46,550 --> 00:07:50,510
+بحثا عن الرطوبة، وتلاحظوا أنه على ال morphology
+
+97
+00:07:50,510 --> 00:07:55,890
+يعني على المربعات كانت ال RBCs تبدو زي كأنها
+
+98
+00:07:55,890 --> 00:07:59,710
+صار لها shrinkage، طبعا يعني صار لها speculated أو
+
+99
+00:07:59,710 --> 00:08:04,170
+crenated، هلأ لحتى بس نعرف إيش يعني speculated أو
+
+100
+00:08:04,170 --> 00:08:08,810
+crenated، بدي بس .. حتى أعرفكم إيش يعني ال .. ال ..
+
+101
+00:08:08,810 --> 00:08:16,350
+إن ال RBCs يصير لها speculated، خليني أحكي
+
+102
+00:08:16,350 --> 00:08:20,330
+speculated
+
+103
+00:08:20,330 --> 00:08:24,230
+RBCs
+
+104
+00:08:24,230 --> 00:08:31,510
+تمام؟ نشوف الصور، إيش يعني؟ هيك high speculated يعني
+
+105
+00:08:31,510 --> 00:08:35,970
+إنها لها زي .. زي أشواك، نتوءات بارزة منها، تمام؟
+
+106
+00:08:35,970 --> 00:08:39,590
+هذه معناه إن ال RBCs صار لها shrinkage، ويمكن
+
+107
+00:08:39,590 --> 00:08:42,850
+كنتوا تشوفوا هذا الشكل، كنتوا تشوفوا إن ال RBCs زي
+
+108
+00:08:42,850 --> 00:08:49,270
+لها هيك أطراف معرجة أو يعني بارز منها بروزات، و
+
+109
+00:08:49,270 --> 00:08:52,590
+أثناء ما كانت تجف العينة في عد ال RBCs، نفس الشيء
+
+110
+00:08:52,590 --> 00:08:57,870
+أنا ممكن أشوفه في حالة ال blood film، لو أنا كنت
+
+111
+00:08:57,870 --> 00:09:00,470
+بستخدم .. لو كان عندي excessive amount of
+
+112
+00:09:00,470 --> 00:09:04,930
+anticoagulant، لو العينة قديمة، أو حتى لو ال ..
+
+113
+00:09:04,930 --> 00:09:12,060
+البيئة حارة فأدت إلى جفاف في العينة بسرعة، تمام؟ طيب،
+
+114
+00:09:12,060 --> 00:09:15,900
+هلأ إيش التجربة اللي أنا بدي أجريها؟ أنا بدي أحط
+
+115
+00:09:15,900 --> 00:09:19,020
+قطرة من الدم، طبعا القطرة هذه لازم يكون حجمها
+
+116
+00:09:19,020 --> 00:09:23,100
+مناسب، ما تكونش كبيرة كثير أو صغيرة كثير، على clean
+
+117
+00:09:23,100 --> 00:09:27,920
+glass slide، على glass slide، شريحة زجاجية، تمام؟ و
+
+118
+00:09:27,920 --> 00:09:32,560
+بدي أجيب شريحة أخرى وأفرد فيها هذا القطر، عشان هيك
+
+119
+00:09:32,560 --> 00:09:38,100
+أنا بستخدم شريحتين زجاجيتين، تمام؟ وطبعا الشريحة
+
+120
+00:09:38,100 --> 00:09:41,460
+اللي أنا بأفرد فيها بكون مثبتاها بزاوية من ثلاثين
+
+121
+00:09:41,460 --> 00:09:45,870
+لأربعين، بتحكم طبعا هذه الزاوية هي اللي بتحكم لي
+
+122
+00:09:45,870 --> 00:09:49,210
+بال thickness of the smear، وهلأ راح نعرف إيش يعني
+
+123
+00:09:49,210 --> 00:09:55,350
+وطبعا بعد ما أنا بأفرد الدم على الشريحة بدي أخليه
+
+124
+00:09:55,350 --> 00:10:02,720
+يجف خلال الهواء، بعدين أنا أحضره للصباغة، هذا كل ما
+
+125
+00:10:02,720 --> 00:10:06,180
+يتعلق بالتجربة، طبعا بيحكي لي هنا أنه ما لازم أنا
+
+126
+00:10:06,180 --> 00:10:12,040
+أنفخ على الشريحة لحتى تجف، بدي أتركها تجف لحالها
+
+127
+00:10:12,040 --> 00:10:16,700
+لأنه اللي بنفخ مع البخار اللي بيطلع ممكن يؤدي إلى
+
+128
+00:10:16,700 --> 00:10:20,160
+RBCs artifact، يعني أن ال RBCs تتشوه أو يصير في
+
+129
+00:10:20,160 --> 00:10:25,380
+عليها أشياء إضافية، ما من .. ما من الخلية ذات نفسها
+
+130
+00:10:26,360 --> 00:10:32,080
+طيب إذا أنا بدي أحكينا place a drop of blood، بدي أحط
+
+131
+00:10:32,080 --> 00:10:36,500
+قطرة .. قطرة من الدم على الشريحة، وباستخدام شريحة
+
+132
+00:10:36,500 --> 00:10:40,880
+أخرى بدي أفرد هذه القطرة، طبعا بهذا الشكل، بأحطها
+
+133
+00:10:40,880 --> 00:10:45,900
+باتجاه معاكس، بخلي قطرة الدم تفرد على طرف الشريحة، و
+
+134
+00:10:45,900 --> 00:10:49,980
+ب .. بحركها بهذا الاتجاه بحيث أنها .. أنه أنا كون
+
+135
+00:10:49,980 --> 00:10:54,280
+فردت العينة، تمام؟ وهيك بيكون شكل ال .. ال .. ال
+
+136
+00:10:54,280 --> 00:10:59,240
+.. مسحة الدم، الدم تم مسحه على الشريحة، وهيك بيكون
+
+137
+00:10:59,240 --> 00:11:03,140
+شكله في النهاية، بتركه to .. to air dry، علشان يجف
+
+138
+00:11:03,140 --> 00:11:09,240
+بالهواء، طيب characteristics of a good smear، إيش هي
+
+139
+00:11:09,240 --> 00:11:14,100
+خواص أو خصائص ال good smear، لحتى أحكي أنا هذا
+
+140
+00:11:14,100 --> 00:11:20,460
+الفيلم أو هذه المسحة للدم جيدة، أو مواصفاتها جيدة،
+
+141
+00:11:20,460 --> 00:11:25,680
+إيش لازم .. إيش الشروط اللي لازم تتحقق فيها؟ أول
+
+142
+00:11:25,680 --> 00:11:31,120
+حاجة، thick at one end, thinning out to a smooth
+
+143
+00:11:31,120 --> 00:11:34,980
+round feather edge، هلأ إيش يعني؟ لازم تكون thick
+
+144
+00:11:34,980 --> 00:11:39,580
+في منطقة و thin في منطقة أخرى، thick في منطقة ال
+
+145
+00:11:39,580 --> 00:11:43,540
+drop، عند منطقة ال drop، هيكون في أعلى تركيز للدم،
+
+146
+00:11:43,540 --> 00:11:49,200
+يعني حتكون thick، سميكة، هيبدأ يخف تركيزه قدام مع أنه
+
+147
+00:11:49,200 --> 00:11:53,360
+كل ما بمسح لقدام شوية، معناه أنه هيكون في عندي أقل
+
+148
+00:11:53,360 --> 00:11:58,680
+تركيز، فبالتالي حتكون هي ذيل، يعني، ولازم تكون
+
+149
+00:11:58,680 --> 00:12:03,060
+أطرافها دائرية ومنظمة، ما .. ما تكون مشرشرة،
+
+150
+00:12:03,060 --> 00:12:06,700
+أطرافها بهذا الشكل، زي ما أنتو شايفين، هي أطرافها
+
+151
+00:12:06,700 --> 00:12:13,870
+دائرية ومنظمة، طيب occupy two thirds of the total
+
+152
+00:12:13,870 --> 00:12:18,610
+slide area، تحتل تقريبا ثلثا الشريحة، وأيضا
+
+153
+00:12:19,700 --> 00:12:22,720
+shouldn't touch any edge of the slide، ما لازم
+
+154
+00:12:22,720 --> 00:12:28,760
+تلامس أي طرف أو أي حافة من حواف الشريحة، تمام؟
+
+155
+00:12:28,760 --> 00:12:34,400
+وكمان should be margin free، ما يكون فيه فراغات، مع
+
+156
+00:12:34,400 --> 00:12:40,940
+دية منطقة القطرة، هذه هنا أنا حطيت القطرة، وفردت
+
+157
+00:12:40,940 --> 00:12:46,020
+الشريحة، مكان القطرة حيصير في عندي زي الفراغات، طبعا
+
+158
+00:12:46,020 --> 00:12:50,260
+هذا مسموح فيه، لكن لازم المسحة تكون منتظمة ومستمرة،
+
+159
+00:12:50,260 --> 00:12:54,800
+إن الدم مفروض بشكل مستمر وما يكون فيه فراغات، should
+
+160
+00:12:54,800 --> 00:13:00,460
+be margin free، طبعا، و as soon as the drop of blood
+
+161
+00:13:00,460 --> 00:13:04,400
+is placed on the glass slide, the smear should be
+
+162
+00:13:04,400 --> 00:13:08,060
+made without delay، مجرد ما أحط قطرة الدم على
+
+163
+00:13:08,060 --> 00:13:12,700
+الشريحة لازم أنا أعمل الفردة أو ال smear، المسحة،
+
+164
+00:13:12,700 --> 00:13:18,600
+بشكل سريع بدون تأخير، لأن أي تأخير راح يؤدي إلى
+
+165
+00:13:18,600 --> 00:13:22,220
+أول حاجة، abnormal distribution of the white blood
+
+166
+00:13:22,220 --> 00:13:29,380
+cells، تمام؟ ثاني شيء، ممكن حواف القطرة تجف، وبالتالي
+
+167
+00:13:29,380 --> 00:13:33,280
+تجرّح الفردة، يعني الفردة تصير فيها فراغات، تصير
+
+168
+00:13:33,280 --> 00:13:38,490
+فيها تجريح، ما تكونش هيك مستمرة وملساء، طبعا
+
+169
+00:13:38,490 --> 00:13:42,990
+فبالتالي بسبب الدم الجاف، فبالتالي أنا لازم أنا
+
+170
+00:13:42,990 --> 00:13:48,090
+أعمل ال .. ال .. الفرد ل .. ل .. لل blood film بشكل
+
+171
+00:13:48,090 --> 00:13:54,070
+سريع، طبعا؟ طيب وحكينا أيضا أن هذا بيأثر ..
+
+172
+00:13:54,070 --> 00:13:56,770
+التأخير في الفرد راح يأثر على distribution of
+
+173
+00:13:56,770 --> 00:14:00,930
+white blood cells، يعني مثلا ال large white cells
+
+174
+00:14:00,930 --> 00:14:04,530
+accumulating at the thin edges of the smear، ممكن
+
+175
+00:14:04,
+
+223
+00:17:58,490 --> 00:18:03,770
+.. ب .. يعني بفرد و ب .. بقفز مع الفرد المتقطع هذا
+
+224
+00:18:03,770 --> 00:18:07,510
+راح تظهر عندي أكثر من منطقة وما بينهم فراغات وهذا
+
+225
+00:18:07,510 --> 00:18:11,050
+غير مقبول، هذا احنا حكينا أنه من شروط الـ blood
+
+226
+00:18:11,050 --> 00:18:14,730
+film أنه يكون margin free ما فيه فراغات، فإذا
+
+227
+00:18:14,730 --> 00:18:20,170
+هذا غلط. طيب بعدين لازم كمان failure to keep the
+
+228
+00:18:20,170 --> 00:18:23,210
+entire edge of the spreader slide against the
+
+229
+00:18:23,210 --> 00:18:26,990
+slide while making the smear. هاي جملة معناها أنه
+
+230
+00:18:26,990 --> 00:18:34,210
+أنا يعني هو بصراحة وأنا بفرد في الـ .. في الـ blood
+
+231
+00:18:34,210 --> 00:18:39,190
+film لازم أنا أكمل فرد لآخر الشريحة يعني ما أوقف
+
+232
+00:18:39,190 --> 00:18:44,780
+فجأة، لحد ما يخلص معي الدم لحاله بي .. بيعطي الدم
+
+233
+00:18:44,780 --> 00:18:48,340
+.. لو أول ما يخلص الدم لحاله بيعطي الشكل الدائري،
+
+234
+00:18:48,340 --> 00:18:52,680
+الطرف الدائري هذا، تمام؟ طيب لو أنا .. يعني ما
+
+235
+00:18:52,680 --> 00:18:56,360
+استمرت بالحركة لآخر الـ slide، توقفت فجأة، إيش رح
+
+236
+00:18:56,360 --> 00:19:01,040
+يصير في عندي؟ رح يصير بهذا الشكل، أنه في عندي الـ
+
+237
+00:19:01,040 --> 00:19:04,860
+.. الـ .. الـ blood film نهايته بدل ما هي دائرية،
+
+238
+00:19:04,860 --> 00:19:09,520
+نهايته لها خط، يعني مثلاً نتوقع .. نتخيل هذا
+
+239
+00:19:09,520 --> 00:19:14,660
+الفيلم هيك وأنا توقفت فجأة نهايته بدل ما هي دائرية
+
+240
+00:19:14,660 --> 00:19:18,960
+هيك خط، تمام؟ فهذا معناها أنه أنا .. أن أنا ما
+
+241
+00:19:18,960 --> 00:19:23,020
+استمرت بالحركة على الـ entire slide يعني ما كملت
+
+242
+00:19:23,020 --> 00:19:29,070
+الحركة للاخر فهذا غلط. failure to keep the spreader
+
+243
+00:19:29,070 --> 00:19:34,610
+slide at 30 degree angle. طبعاً إذا أنا ما .. يعني
+
+244
+00:19:34,610 --> 00:19:38,110
+ما خلت الزاوية ثلاثين أو من ثلاثين لأربعين فهذا
+
+245
+00:19:38,110 --> 00:19:41,650
+معناها أنه أنا إذا كبرت الزاوية رح يكون عندي short و
+
+246
+00:19:41,650 --> 00:19:48,570
+thick أو إذا صغرتها رح يكون عندي thin و long وهذا
+
+247
+00:19:48,570 --> 00:19:52,680
+غلط. تمام؟ أنا فعلياً بس بكبر الزاوية في حالة الـ
+
+248
+00:19:52,680 --> 00:19:56,120
+decreased hematocrit وبصغر الزاوية في حالة الـ
+
+249
+00:19:56,120 --> 00:19:59,780
+increased hematocrit. شخص normal أو ما عنده المشكلة
+
+250
+00:19:59,780 --> 00:20:03,880
+هاي فبالتالي لازم الزاوية تكون من 30 لـ 40. failure
+
+251
+00:20:03,880 --> 00:20:06,900
+to push the spreader slide completely across the
+
+252
+00:20:06,900 --> 00:20:11,030
+slide. حكينا إذا أنا ما .. إذا ما حطيت الشريحة اللي
+
+253
+00:20:11,030 --> 00:20:17,290
+أنا بفرد فيها ما كملت حركتها لآخر الشريحة اللي أنا
+
+254
+00:20:17,290 --> 00:20:22,750
+بفرد عليها فبالتالي هذا برضه كمان راح يعطيني smear
+
+255
+00:20:22,750 --> 00:20:27,170
+نهايته مش دائرية. irregular spread with ridges and
+
+256
+00:20:27,170 --> 00:20:33,030
+long tail. إذا كان في عندي الـ .. الفرد مش منتظم و
+
+257
+00:20:33,030 --> 00:20:38,770
+فيه حواف أو فيه زي أطراف يعني مش منتظمة
+
+258
+00:20:38,770 --> 00:20:43,750
+هذا معناه أنه الشريحة اللي أنا بفرد فيها هي إما
+
+259
+00:20:43,750 --> 00:20:50,150
+dirty عليها غبرة أو ممكن تكون مكسورة يعني في ..
+
+260
+00:20:50,150 --> 00:20:56,300
+فيها خدوش أو كسور فهذا برضه كمان ممكن يأثر. Hole
+
+261
+00:20:56,300 --> 00:21:01,560
+in films. إذا حسيت أنه الـ blood film فيه حفورات
+
+262
+00:21:01,560 --> 00:21:07,180
+فهذا معناها أن الشريحة عليها fat أو عليها دهون
+
+263
+00:21:07,180 --> 00:21:12,080
+تمام، عليها زيت مثلاً فهذه لازم أنا أنظفها بالكحول و
+
+264
+00:21:12,080 --> 00:21:16,820
+أستخدمها بعدين أو أنا أستخدم يعني نوعية أفضل. في
+
+265
+00:21:16,820 --> 00:21:20,580
+عندي بعض الشرائح هي already أصلاً تيجي محفوظة بشمع
+
+266
+00:21:21,050 --> 00:21:24,790
+فيعني عشان ما تجرح بعضها البعض فبيكون في عليها طبقة
+
+267
+00:21:24,790 --> 00:21:30,530
+رفيعة من الشمع. فالمفروض أنه إذا كانت الشريحة أو
+
+268
+00:21:30,530 --> 00:21:34,990
+الشرائح اللي موجودة عندي من هذا النوع فالمفروض أن
+
+269
+00:21:34,990 --> 00:21:38,630
+أنا أنقعها في الكحول ليلة قبل ما أستخدمها يعني
+
+270
+00:21:38,630 --> 00:21:45,210
+overnight، تكون محطوطة في .. في jar في كحول قبل
+
+271
+00:21:45,210 --> 00:21:48,910
+الاستخدام علشان الكحول يعمل على إذابة الشمع اللي
+
+272
+00:21:48,910 --> 00:21:52,390
+موجود عليها. Cellular degenerative changes. إذا كان
+
+273
+00:21:52,390 --> 00:22:00,990
+في عندي تأخير في عملية الـ fixation في تثبيت الفيلم
+
+274
+00:22:00,990 --> 00:22:04,110
+على الشريحة أو حتى الـ fixative اللي أنا بستخدمه
+
+275
+00:22:04,110 --> 00:22:07,790
+اللي هو الـ methanol إذا تم تلويثه بماء أو يعني
+
+276
+00:22:07,790 --> 00:22:11,190
+إجى عليه ماء أو امتص رطوبة فهذا راح يأثر على
+
+277
+00:22:11,190 --> 00:22:14,210
+الخلايا ممكن يقود إلى تحللها تمام؟ يعمل لي
+
+278
+00:22:14,210 --> 00:22:18,070
+cellular degenerative changes. إذا هذه كلها أسباب
+
+279
+00:22:18,070 --> 00:22:22,760
+ممكن إنها تأثر على جودة الـ blood film. هي هذه الـ في
+
+280
+00:22:22,760 --> 00:22:26,000
+either edge أو irregular edges يعني أطراف مشرشرة
+
+281
+00:22:26,000 --> 00:22:30,160
+هذا نتيجة أنه إما الشريحة مش نظيفة عليها غبرة أو
+
+282
+00:22:30,160 --> 00:22:33,520
+أن الشريحة اللي أنا بفرد فيها أطرافها مكسورة أو في
+
+283
+00:22:33,520 --> 00:22:37,180
+عليها شقوق. هذا الـ jerky manner اللي هو الأسلوب
+
+284
+00:22:37,180 --> 00:22:41,580
+المتقطع هذا أنه يعني يُقال أنه كويس بس المفروض أنه
+
+285
+00:22:41,580 --> 00:22:45,300
+يكون أطول شوية. هذا too short قليل جداً نتيجة أنه
+
+286
+00:22:45,300 --> 00:22:52,250
+أنا مثلاً كبرت الزاوية. هذا رقيق كثير، مشكلته أنه
+
+287
+00:22:52,250 --> 00:22:56,150
+يعني لازم يكون هو أعرض من هيك شوية. هذه فيها holes
+
+288
+00:22:56,150 --> 00:23:03,470
+نتيجة أن الشريحة عليها شمع أو عليها زيت. تمام. oil
+
+289
+00:23:03,470 --> 00:23:09,470
+هذه أنه معناها .. هذه الشكل يعني أنه إيدي مالت هيك
+
+290
+00:23:09,470 --> 00:23:13,640
+انحرفت باتجاه معين وأنا بفرد، فلازم أنا أضل على
+
+291
+00:23:13,640 --> 00:23:18,820
+الـ entire هيك ما أـ .. ما أميل وأضل باتجاه يعني
+
+292
+00:23:18,820 --> 00:23:22,980
+مستقيم. وهذا هيو بيحكي لك failure to keep the
+
+293
+00:23:22,980 --> 00:23:25,720
+entire edge of the spreader slide against the
+
+294
+00:23:25,720 --> 00:23:31,800
+slide while making the smear. يعني لازم لحظة
+
+295
+00:23:33,580 --> 00:23:39,080
+لازم حركة الـ .. الـ .. الشريحة الفاردة اللي بفرد
+
+296
+00:23:39,080 --> 00:23:47,080
+فيها تكون يعني على نفس اتجاه الشريحة، تمام؟ الحركة
+
+297
+00:23:47,080 --> 00:23:49,860
+على نفس الاتجاه، ولكن لما أنا ببتعد، ببتعد
+
+298
+00:23:49,860 --> 00:23:53,140
+بالاتجاه المعاكس، وهذا الكلام كله رح أورjيكم إياه في
+
+299
+00:23:53,140 --> 00:23:56,460
+العمل، إيش معناه؟ تمام؟ هنا في عندي air drop عشان
+
+300
+00:23:56,460 --> 00:24:01,980
+هيك طلعت في عندي منطقة فاضية في نص القطرة أو في نص
+
+301
+00:24:01,980 --> 00:24:07,970
+الفيلم. تمام. biological causes of a poor smear يعني
+
+302
+00:24:07,970 --> 00:24:14,430
+أسباب يعني بتؤدي إلى أنه السمير يكون سيئ أو رديء
+
+303
+00:24:14,430 --> 00:24:20,110
+ولكن المشكلة في العينة وليس في تحضير الفيلم. أول
+
+304
+00:24:20,110 --> 00:24:27,000
+شيء called agglutination. مشكلة أن الـ RBCs إذا ما كانت
+
+305
+00:24:27,000 --> 00:24:30,620
+موجودة على درجة حرارة أقل من 37 أو على درجة حرارة
+
+306
+00:24:30,620 --> 00:24:36,620
+الغرفة مثلاً ممكن أن الدم يتجمع أو يتجلط حتى لو
+
+307
+00:24:36,620 --> 00:24:39,300
+أنا حاطة فيه anticoagulant ممكن يتجلط نتيجة وجود
+
+308
+00:24:39,300 --> 00:24:42,340
+antibody بيجمع الـ RBCs مع بعضها البعض وبيؤدي إلى
+
+309
+00:24:42,340 --> 00:24:47,640
+aggregation. فالحل أنه طبعاً هذا الـ antibody بيشتغل
+
+310
+00:24:47,640 --> 00:24:51,340
+على درجة حرارة منخفضة يعني درجة حرارة الغرفة فأقل
+
+311
+00:24:51,340 --> 00:24:56,530
+من 25 وتحت. طيب فالحل أنه أنا أدفئ العينة أحطها على
+
+312
+00:24:56,530 --> 00:25:00,230
+37 علشان هذا الـ antibody يفك عن الخلايا ويُبطل
+
+313
+00:25:00,230 --> 00:25:05,610
+يُجمعها ويُبطل في عندي agglutination. تمام؟ فالفكرة
+
+314
+00:25:05,610 --> 00:25:10,770
+أن أنا لو لاحظت أن في شخص عنده هذه المشكلة عنده
+
+315
+00:25:10,770 --> 00:25:14,450
+antibody ممكن يجمع الـ RBC يعني عنده حالة called
+
+316
+00:25:14,450 --> 00:25:18,330
+agglutination. تمام؟ لازم أن أنا أعمل incubation للـ
+
+317
+00:25:18,330 --> 00:25:22,690
+RBCs على 37 درجة أحطها مثلاً في الـ water bath أو في
+
+318
+00:25:22,690 --> 00:25:26,190
+الـ incubator بعدين أدفئ العينة بعدين أحضر منها
+
+319
+00:25:26,190 --> 00:25:33,280
+السمير. ليبيميا إذا الشخص عنده دهون عالية تمام
+
+320
+00:25:33,280 --> 00:25:36,480
+lipemia يعني نسبة الدهون عالية أو مستوى الدهون
+
+321
+00:25:36,480 --> 00:25:40,880
+عالي تمام فهذا معناه أنه راح يظهر holes حفورات في
+
+322
+00:25:40,880 --> 00:25:44,260
+الـ blood film. هذه بصراحة المشكلة أنا ما أقدر يعني
+
+323
+00:25:44,260 --> 00:25:50,570
+أحلها لو كانت المشكلة على الفيلم. إذا كان الـ .. يعني
+
+324
+00:25:50,570 --> 00:25:53,410
+إذا كانت مشكلة الدهون موجودة على الشريحة فأنا ممكن
+
+325
+00:25:53,410 --> 00:25:55,850
+أنظف الشريحة. بس إذا كانت الدهون موجودة في العينة
+
+326
+00:25:55,850 --> 00:26:01,290
+ذات نفسها أنا ما .. يعني لا أستطيع أن أنا أصحح هذا
+
+327
+00:26:01,290 --> 00:26:07,250
+الخطأ أو هذه المشكلة. Rouleau Formation. إذا الـ RBCs
+
+328
+00:26:07,250 --> 00:26:10,810
+وإنها هي بتعمل stack resembling coins. هذا طبعاً
+
+329
+00:26:10,810 --> 00:26:13,570
+تعريف الـ Rouleau Formation وأظن أنه مر معنا في
+
+330
+00:26:13,570 --> 00:26:18,950
+معمل الـ ESR. طبعاً هذا نتيجة أنه في عندي بروتينات
+
+331
+00:26:18,950 --> 00:26:21,970
+عالية بتُقلل المسافة ما بين الـ RBCs
+
+332
+00:26:21,970 --> 00:26:25,310
+بتؤدي إلى تجمعها والـ .. والبروتينات هذه بتكون
+
+333
+00:26:25,310 --> 00:26:28,330
+مرتبطة بحالات مرضية معينة زي الـ infection والـ
+
+334
+00:26:28,330 --> 00:26:31,380
+inflammation والـ tissue injury. والصراحة أنا ..
+
+335
+00:26:31,380 --> 00:26:34,820
+يعني إذا الشخص عنده مشكلة مرضية فأنا ما أقدر أحلها
+
+336
+00:26:34,820 --> 00:26:40,300
+لحتى أمنع أن يتكون الـ rouleau أثناء فرد العينة
+
+337
+00:26:40,300 --> 00:26:47,720
+تمام؟ فهذا طبعاً الـ .. الشيء ما أقدر أحله. نقرأ
+
+338
+00:26:47,720 --> 00:26:52,500
+الشريحة وبنعملها examination وهي موجودة لهذه
+
+339
+00:26:52,500 --> 00:26:57,380
+المشكلة عادي يعني بنحاول نعمل .. نعملها skip. طيب
+
+340
+00:26:59,340 --> 00:27:03,800
+although this is the easiest and most popular
+
+341
+00:27:03,800 --> 00:27:07,020
+method for producing a blood smear it doesn't
+
+342
+00:27:07,020 --> 00:27:11,680
+produce a quality smear as a result of. هلأ صح الـ ..
+
+343
+00:27:11,680 --> 00:27:15,320
+الـ smear. طريقة الـ smear اللي هي السمير اللي بيتحضر
+
+344
+00:27:15,320 --> 00:27:20,280
+بـ two slides هي أفضل طريقة .. يعني أسهل طريقة هي
+
+345
+00:27:20,280 --> 00:27:25,010
+الـ easiest الأسهل والـ most popular الأكثر شيوعاً
+
+346
+00:27:25,010 --> 00:27:29,870
+ولكن هي ما بتعطيني يعني smear جودته عالية جداً
+
+347
+00:27:29,870 --> 00:27:34,270
+مقارنة مثلاً بالـ automated بالـ .. بالـ spun تمام؟
+
+348
+00:27:34,270 --> 00:27:41,690
+ليش؟ لأنه أول حاجة الـ white blood cells أحياناً ما بتتوزعش
+
+349
+00:27:41,690 --> 00:27:44,350
+بشكل منتظم وممكن الـ .. والـ RBCs بيصير لها
+
+350
+00:27:44,350 --> 00:27:49,690
+distortion بتتشوه شكلها على الحواف. أيضاً إذا كانت
+
+351
+00:27:49,690 --> 00:27:56,990
+أطراف السمير مشرشرة زي هيك فإنه في عندي .. أن الـ
+
+352
+00:27:56,990 --> 00:27:59,410
+.. الـ smaller white blood cells زي الـ lymphocytes الـ
+
+353
+00:27:59,410 --> 00:28:02,850
+smaller white blood cells زي الـ lymphocytes بتكون موجودة
+
+354
+00:28:02,850 --> 00:28:07,170
+في .. في منتصف هذه الأطراف المشرشرة والـ large
+
+355
+00:28:07,170 --> 00:28:12,610
+lymphocytes اللي هي زي الـ monocytes بتكون موجودة على
+
+356
+00:28:12,610 --> 00:28:16,430
+الحواف. تمام؟ بتكون موجودة على الحواف يعني لو نيجي
+
+357
+00:28:16,430 --> 00:28:20,280
+هنا هنا مثلاً على هذه الحافة في المنتصف فيه
+
+358
+00:28:20,280 --> 00:28:24,760
+lymphocytes وعلى الحواف على الأطراف بيكون موجود الـ
+
+359
+00:28:24,760 --> 00:28:28,080
+.. الـ monocytes اللي هي الـ large lymphocytes .. الـ
+
+360
+00:28:28,080 --> 00:28:32,580
+large white blood cells. Spun Smear reproduce the most
+
+361
+00:28:32,580 --> 00:28:35,460
+uniform distribution of blood cells. يعني الـ Spun
+
+362
+00:28:35,460 --> 00:28:39,920
+Smear أو الـ automated Smear بيعطيني يعني توزيع
+
+363
+00:28:39,920 --> 00:28:46,620
+أفضل للـ .. للـ .. للـ blood cells. طيب حضرت أنا الـ ..
+
+364
+00:28:46,620 --> 00:28:52,880
+الشريحة، فردت الـ blood عملت فيلم وخليته air dry
+
+365
+00:28:52,880 --> 00:28:57,990
+يجف بالهواء. هلأ دور أني أنا أعمل fixation and
+
+366
+00:28:57,990 --> 00:29:02,150
+staining. تمام؟ fixation and staining. هلأ بنعمل
+
+367
+00:29:02,150 --> 00:29:05,690
+fixation تثبيت لهذه العينة أو لهذا الدم على
+
+368
+00:29:05,690 --> 00:29:10,750
+الشريحة. بدي أستخدم fixative. تمام؟ وهذا الـ
+
+369
+00:29:10,750 --> 00:29:14,190
+fixative إيش دوره؟ preserve the morphology of cells
+
+370
+00:29:14,190 --> 00:29:19,650
+بيحافظ على شكل الخلايا. تمام؟ علشان أظلّها محتفظة
+
+371
+00:29:19,650 --> 00:29:23,290
+بشكلها ولما أجي أنا أدرس الـ morphology تبعها يعني
+
+372
+00:29:23,290 --> 00:29:26,630
+أكون شكلها معبر زي كأنها موجودة في الـ circulation
+
+373
+00:29:26,630 --> 00:29:33,770
+فبالتالي يعني أشخص صح. طيب إذا الـ .. الـ .. الـ films
+
+374
+00:29:33,770 --> 00:29:38,490
+must be fixed. لازم .. لازم أثبتها وعلى طول أول ما
+
+375
+00:29:38,490 --> 00:29:42,210
+تجف لازم أثبتها باستخدام fixative. الـ fixative
+
+376
+00:29:42,210 --> 00:29:45,550
+اللي أنا بستخدمه الـ methyl alcohol اللي هو الـ
+
+377
+00:29:45,550 --> 00:29:49,690
+methanol أو ممكن أستخدم الـ ethyl alcohol اللي هو
+
+378
+00:29:49,690 --> 00:29:53,290
+الـ ethanol. طبعاً لازم يكون absolute. الـ methanol
+
+379
+00:29:53,290 --> 00:29:56,670
+طبعاً اللي أنا بستخدمه لازم يكون pure والـ ethanol
+
+380
+00:29:56,670 --> 00:30:00,610
+طبعاً أنتم مار عليكم 70% ethanol يعني أنا
+
+381
+00:30:00,610 --> 00:30:04,850
+بحضر مثلاً باخذ 70% من الـ ethanol وبحط عليه 30
+
+382
+00:30:04,850 --> 00:30:12,990
+water. تمام؟ لا، لكن كلمة absolute هذا معناها أنه
+
+383
+00:30:12,990 --> 00:30:20,270
+الـ ethyl يعني تقريباً 99.9% pure ما عليه أي قطرة
+
+384
+00:30:20,270 --> 00:30:24,250
+ماء. تمام؟ والـ methanol لازم أيضاً يكون pure مش
+
+385
+00:30:24,250 --> 00:30:29,010
+مخلوط بماء زي ما بنشتريه من الشركة ما يكون عليه
+
+386
+00:30:29,010 --> 00:30:34,730
+ماء أو معمول له dilution بالماء نهائياً. تمام؟ ليش؟
+
+387
+00:30:34,730 --> 00:30:39,920
+لأن الماء بيؤدي إلى تغيرات، degenerative changes،
+
+388
+00:30:39,920 --> 00:30:44,580
+بيؤدي إلى تحلل الخلايا أو تكسيرها أو ممكن يعمل لي
+
+389
+00:30:44,580 --> 00:30:51,520
+artifact، يعيق عملية الرؤية، فبالتالي لازم يكون الـ
+
+390
+00:30:51,520 --> 00:30:55,140
+fixative pure علشان تكون جودته عالية ويثبت لي
+
+391
+00:30:55,140 --> 00:30:58,480
+الخلايا ويعمل لي preserve للـ morphology بشكل جيد
+
+392
+00:31:03,360 --> 00:31:07,400
+طبعاً أنا بكون حاطة هذا الكحول في jar في مرتبان
+
+393
+00:31:07,400 --> 00:31:12,300
+زجاجي وهذا المرتبان فيه فرزات لأنه تجلس الشريحة
+
+394
+00:31:12,300 --> 00:31:16,620
+فيه. فبالتالي والمرتبان بيكون بيستوعب أكثر من
+
+395
+00:31:16,620 --> 00:31:21,880
+شريحة فبحط الش
+
+445
+00:34:59,340 --> 00:35:03,020
+تأثر على ال diagnosis to prevent the alcohol from
+
+446
+00:35:03,020 --> 00:35:07,740
+becoming contaminated by absorbed water. طبعا علشان
+
+447
+00:35:07,740 --> 00:35:12,620
+أمنع الكحول من أن يمتص ماء أو رطوبة من الجو أو
+
+448
+00:35:12,620 --> 00:35:16,600
+يصبح contaminated بماء. لازم أن يكون موجود
+
+449
+00:35:16,600 --> 00:35:20,540
+في bottle with a tightly fitting stopper، ويكون مغطى
+
+450
+00:35:20,540 --> 00:35:26,090
+بشكل جيد جدًا، and not left exposed to the
+
+451
+00:35:26,090 --> 00:35:30,490
+atmosphere. وأنا مثلاً ما بنفع أحطه في الجار وأترك
+
+452
+00:35:30,490 --> 00:35:35,610
+الجار مفتوح؛ لهيك بيمتص رطوبة وبيؤثر على الشغل،
+
+453
+00:35:35,610 --> 00:35:38,270
+تمام؟ وخصوصاً في الـ humid climates، خصوصاً في
+
+454
+00:35:38,270 --> 00:35:43,510
+الأجواء الرطبة أو في الرطوبة العالية. تمام، إذًا هيك
+
+455
+00:35:43,510 --> 00:35:47,310
+إحنا خلصنا الـ fixation. ثبتنا الخلايا واحتفظنا
+
+456
+00:35:47,310 --> 00:35:51,810
+بِشكلها ومنعنا وصول الماء إليها. بعد هيك، بعد الـ fixation
+
+457
+00:35:51,810 --> 00:35:55,370
+غسلناها، مش مشكلة. بس قبل ذلك، ممنوع يلامسها ماء أو
+
+458
+00:35:55,370 --> 00:35:59,830
+يكون فيه الـ methanol أو الـ ethanol ملوث بالماء. طيب،
+
+459
+00:35:59,830 --> 00:36:05,070
+بعد هيك بدي أنا أبدأ أصبغ الشريحة أو أصبغ الفيلم.
+
+460
+00:36:05,070 --> 00:36:09,790
+بدي أستخدم Romanovski stains. تمام؟ إيش يعني
+
+461
+00:36:09,790 --> 00:36:12,450
+Romanovski stains؟ هلأ هالـ Romanovski
+
+462
+00:36:12,450 --> 00:36:16,910
+stains هي عبارة عن عائلة من الصبغات. تمام؟ هي يعني
+
+463
+00:36:16,910 --> 00:36:20,750
+مش صبغة واحدة؛ هي مجموعة. هو مصطلح يُطلق على
+
+464
+00:36:20,750 --> 00:36:24,790
+مجموعة من الصبغات، عائلة من الصبغات. تمام؟ هاي
+
+465
+00:36:24,790 --> 00:36:31,840
+الصبغات تُستخدم لصبغ الـ blood film، وبتعطيني
+
+466
+00:36:31,840 --> 00:36:36,400
+نتائج very satisfactory. تمام؟ ومن أمثلة هذه
+
+467
+00:36:36,400 --> 00:36:40,840
+الصبغات اللي تنتمي لعائلة الـ رومانوفسكي ستينز، الـ Giemsa
+
+468
+00:36:40,840 --> 00:36:45,180
+stain. تمام؟ وهي مهمة، يعني
+
+469
+00:36:45,180 --> 00:36:49,660
+كثير مناسبة للـ routine work، للشغل الروتيني، الـ
+
+470
+00:36:49,660 --> 00:36:54,080
+Giemsa stain بتستخدم
+
+471
+00:36:54,080 --> 00:36:57,680
+طبعًا بتعطيني delicate stain، يعني تفاصيل جيدة جدًا.
+
+472
+00:36:59,740 --> 00:37:04,680
+Leishman’s stain. Leishman’s هي عبارة عن the
+
+473
+00:37:04,680 --> 00:37:08,520
+simplest method، أبسط طريقة، مش أفضل، أبسط
+
+474
+00:37:08,520 --> 00:37:12,140
+طريقة. يعني في أفضل منها، بس هي أبسط طريقة و
+
+475
+00:37:12,140 --> 00:37:16,940
+أسهل طريقة تُستخدم لتحضير الـ blood film، وإحنا
+
+476
+00:37:16,940 --> 00:37:20,700
+بنستخدمها لما بيكون الـ blood film مطلوب قراءته
+
+477
+00:37:20,700 --> 00:37:23,860
+urgently. إيش يعني كلمة urgently؟ هتمرّ معكم كثير
+
+478
+00:37:23,860 --> 00:37:29,800
+خلال دراستكم. التحليل urgently يعني فحص عاجل، أو شو
+
+479
+00:37:29,800 --> 00:37:33,770
+يعني؟ إحضار عينة عاجلاً، لازم أنت تقرأها
+
+480
+00:37:33,770 --> 00:37:37,590
+لأنّه بيكون الشخص يعني حالته حرجة جدًا، ما بين الحياة
+
+481
+00:37:37,590 --> 00:37:42,070
+والموت، فأنت لازم تشتغلي له فحوصاته urgently. تمام؟
+
+482
+00:37:42,070 --> 00:37:46,110
+يعني طارئ، أو يعني عاجل. هاي معنى كلمة urgently.
+
+483
+00:37:46,110 --> 00:37:53,050
+تمام؟ وطبعًا ليش أنا بستخدمها مع الـ urgent
+
+484
+00:37:53,050 --> 00:38:00,360
+cases أو الـ urgent samples؟ لأنها الصبغة منها
+
+485
+00:38:00,360 --> 00:38:04,700
+وفيها fixative وصبغة، يعني هي 2 في 1. يعني هي الصبغة
+
+486
+00:38:04,700 --> 00:38:08,760
+نفسها، الـ powder تم adapt بـ methanol، فأنا ما بحتاج
+
+487
+00:38:08,760 --> 00:38:12,180
+لخطوة fixation. يعني أنا وفرت من 2 لـ 3 minutes في الـ
+
+488
+00:38:12,180 --> 00:38:16,700
+fixation. فبحط على طول، يعني بعد ما أفرغ العينة،
+
+489
+00:38:16,700 --> 00:38:21,320
+وأخليها air dry، تمام، على طول بصبغها بالـ Leishman’s. ليش؟
+
+490
+00:38:21,320 --> 00:38:24,800
+لأنها فيها صبغة وفيها ميثانول، هتثبت وتصبغ في
+
+491
+00:38:24,800 --> 00:38:28,420
+نفس الوقت. عشان هيك إحنا بنحكي عنها simple، بسيطة، و
+
+492
+00:38:28,420 --> 00:38:31,540
+بتنفع للـ urgent cases، لحالات الطارئة، لأنها ما بتأخذ
+
+493
+00:38:31,540 --> 00:38:34,640
+وقت، خصوصًا الشغل اللي بيجي at night مثلاً، يعني في
+
+494
+00:38:34,640 --> 00:38:38,040
+الليل، عينة بُعتت في الليل، بدنا نقرأ لها blood film،
+
+495
+00:38:38,040 --> 00:38:41,180
+أكيد. يعني هي طريقة سريعة، يعني المريض بيكون وضع
+
+496
+00:38:41,180 --> 00:38:45,650
+حرج، عشان هيك بُعتت. Field stain، صبغة الـ field
+
+497
+00:38:45,650 --> 00:38:53,090
+طبعًا هي يعني صبغة سريعة تُستخدم لـ thin
+
+498
+00:38:53,090 --> 00:38:56,770
+films أو الـ thick films of parasites، malaria، الـ
+
+499
+00:38:56,770 --> 00:39:00,950
+parasites، تمام؟ تُستخدم لتشخيص الطفيليات، خصوصاً
+
+500
+00:39:00,950 --> 00:39:08,200
+الملاريا. هلأ كيف تشتغل الـ Romanovski stains؟ يعني سواء
+
+501
+00:39:08,200 --> 00:39:12,700
+كانت Giemsa أو Wright أو كانت Leishman’s أو whatever، إيش
+
+502
+00:39:12,700 --> 00:39:17,600
+نوعها، تمام؟ إيه كيف تشتغل؟ هلأ طالما أن هي تنتمي
+
+503
+00:39:17,600 --> 00:39:21,900
+لعائلة الـ Romanovski stains، إذا هي عبارة عن صبغة
+
+504
+00:39:21,900 --> 00:39:27,660
+مركبة، موجود فيها جزء حامضي وجزء قاعدي، تمام؟ الجزء
+
+505
+00:39:27,660 --> 00:39:32,020
+القاعدي بسميه cationic أو basic dye، زي الميثيلين
+
+506
+00:39:32,020 --> 00:39:36,450
+blue، أو أحد مشتقات الأكسدة تبعته، اللي هو الـ
+
+507
+00:39:36,450 --> 00:39:41,870
+oxidation product للميثيلين بلو، زي الـ Azur. طبعًا
+
+508
+00:39:41,870 --> 00:39:46,490
+سواء كانت ميثيلين بلو أو Azur، طبعًا هي صبغة
+
+509
+00:39:46,490 --> 00:39:51,590
+قاعدية، cationic. طبعًا يعني شحنتها موجبة، فإذا هي
+
+510
+00:39:51,590 --> 00:39:56,070
+هتصبغ الـ anionic أو الـ acidic أو
+
+511
+00:39:56,070 --> 00:40:01,400
+الأجزاء اللي شحنتها سالبة في الخلية. تمام؟ إيش اللي
+
+512
+00:40:01,400 --> 00:40:04,140
+هي الأجزاء الحامضية أو الأجزاء اللي شحنتها سالبة
+
+513
+00:40:04,140 --> 00:40:09,560
+في الخلية؟ ما فيش أشهر من الأحماض النووية، الـ
+
+514
+00:40:09,560 --> 00:40:13,780
+nucleic acid، الـ DNA والـ RNA، فبتصبغهم وبتعطيهم
+
+515
+00:40:13,780 --> 00:40:17,400
+اللون ما بين blue لـ grey. تمام؟ أحيانًا بتعطيهم
+
+516
+00:40:17,400 --> 00:40:22,380
+اللون الـ violet. تمام؟ أيضًا للـ nucleoproteins،
+
+517
+00:40:22,380 --> 00:40:28,480
+بروتينات النواة، وحبيبات الـ basophil برضه كمان
+
+518
+00:40:28,480 --> 00:40:33,000
+بتنصبغ فيها، وأحيانًا يعني بشكل ضعيف جدًا حبيبات الـ
+
+519
+00:40:33,000 --> 00:40:36,580
+neutrophil بتأخذ هاي الصبغة. طيب، هلأ إيش يعني
+
+520
+00:40:36,580 --> 00:40:39,300
+حبيبات الـ basophil؟ مش إحنا حكينا إنه الـ
+
+521
+00:40:39,300 --> 00:40:43,170
+neutrophil والـ basophil والـ eosinophil، تمام، هدول
+
+522
+00:40:43,170 --> 00:40:47,830
+الثلاث خلايا بنحكي عنهم granulocytes، يعني خلايا
+
+523
+00:40:47,830 --> 00:40:51,870
+محببة، الـ cytoplasm تبعهم فيهم حبيبات؟ اه، طيب،
+
+524
+00:40:51,870 --> 00:40:56,650
+الـ basophil، ليش سميناها أصلاً basophil؟ لأن حبيباتها
+
+525
+00:40:56,650 --> 00:41:04,200
+عبارة عن حبيبات محبة للـ basic dye، تمام؟ إذا هي
+
+526
+00:41:04,200 --> 00:41:08,460
+أخذت اسمها من ظهورها على الـ blood film، يعني ليش
+
+527
+00:41:08,460 --> 00:41:12,200
+سميناها basophil؟ لأنها تحتوي على حبيبات، phil يعني
+
+528
+00:41:12,200 --> 00:41:15,780
+محبة، تمام؟ جاي من كلمة philic، محبة. لما نقول
+
+529
+00:41:15,780 --> 00:41:21,070
+hydrophilic، محبة للماء، تمام؟ phil محبة، و baso
+
+530
+00:41:21,070 --> 00:41:26,250
+محبة للقاعدة. إذا حبيباتها، تركيبها حامض، محبة
+
+531
+00:41:26,250 --> 00:41:31,890
+للصبغة القاعدية، اللي هي الـ basic dye، اللي هي الـ
+
+532
+00:41:31,890 --> 00:41:38,140
+methylene blue. تمام؟ طيب، وإذًا هل هذا هو المكون
+
+533
+00:41:38,140 --> 00:41:42,460
+الوحيد للصبغة؟ لأ، في مكون آخر بيكون موجود فيها
+
+534
+00:41:42,460 --> 00:41:47,360
+اللي هو الشق الحامضي، أو الـ anionic، أو اللي هو سالب
+
+535
+00:41:47,360 --> 00:41:52,420
+الشحنة، زي الـ eosin Y والـ eosin B، مثال على هذا الشق
+
+536
+00:41:52,420 --> 00:41:57,280
+أو النوع، اللي هي صبغة eosin Y و eosin B. طبعًا هدا
+
+537
+00:41:57,280 --> 00:42:03,940
+الجزء أو هذا التكوين من الصبغة بيصبغ لي الـ
+
+538
+00:42:03,940 --> 00:42:10,500
+cationic، طبعًا هو سالب، فهيرتبط أو يصبغ
+
+539
+00:42:10,500 --> 00:42:16,760
+الأجزاء اللي شحنتها موجبة، زي الـ hemoglobin و
+
+540
+00:42:16,760 --> 00:42:19,740
+الـ azinophilic granules، وهيعطيها اللون ما بين الـ
+
+541
+00:42:19,740 --> 00:42:25,070
+orange للـ red. تمام؟ طيب، الـ azinophilic granules نفس
+
+542
+00:42:25,070 --> 00:42:29,950
+فكرة الـ basophil. هلأ الـ azinophilic هي خلية
+
+543
+00:42:29,950 --> 00:42:34,290
+granulocyte، في الـ cytoplasm تبعها حبيبات،
+
+544
+00:42:34,290 --> 00:42:39,890
+الحبيبات هذه، phil لمين؟ محبة لمين؟ للـ eosin، اللي
+
+545
+00:42:39,890 --> 00:42:44,230
+هي للـ acidic dye، عشان هيك سميناها azinophilic granules.
+
+546
+00:42:44,230 --> 00:42:49,040
+أو azinophilic cells. تمام؟ إذا الصبغة بتكون موجود فيها
+
+547
+00:42:49,040 --> 00:42:53,240
+يعني سواء كانت Giemsa, Wright, Leishman’s, Field، أي
+
+548
+00:42:53,240 --> 00:42:56,520
+نوع من هذه الصبغات، هيكون فيه methylene blue أو أحد
+
+549
+00:42:56,520 --> 00:42:59,920
+مشتقات الأكسدة تبعته، زي الـ Azur، وهيكون فيها
+
+550
+00:42:59,920 --> 00:43:05,900
+eosin Y و eosin B. الـ methylene blue هو قاعدي وشحنته
+
+551
+00:43:05,900 --> 00:43:10,060
+موجبة، هيصبغ الأشياء السالبة في الشحنة، اللي هي
+
+552
+00:43:10,060 --> 00:43:13,480
+الحامضية، زي الـ DNA والـ RNA والـ nucleoproteins،
+
+553
+00:43:14,420 --> 00:43:20,660
+وحبيبات الـ basophil. تمام؟ طيب، أما الـ
+
+554
+00:43:20,660 --> 00:43:24,860
+الجزء الحامضي، اللي هو سالب الشحنة، الـ anionic، زي
+
+555
+00:43:24,860 --> 00:43:30,560
+الـ eosin Y والـ eosin B، هيرتبط أو يصبغ بالـ cationic sites، أو
+
+556
+00:43:30,560 --> 00:43:34,320
+اللي هو إيه؟ يعني الأجزاء القاعدية الموجبة الشحنة
+
+557
+00:43:34,320 --> 00:43:37,830
+في الخلية، ويعطيها اللون الـ orange للـ red، زي الـ
+
+558
+00:43:37,830 --> 00:43:41,010
+hemoglobin اللي موجود في الـ RBCs، هو قاعدي، يرتبط بالـ
+
+559
+00:43:41,010 --> 00:43:44,870
+ينصبغ بالـ eosin، وأيضًا الـ azinophilic granules.
+
+560
+00:43:44,870 --> 00:43:49,310
+حبيبات الـ azinophil. طيب، حبيبات الـ neutrophil من اسمها
+
+561
+00:43:49,310 --> 00:43:53,550
+neutral، متعادلة، فمحبتها يعني بتنصبغ
+
+562
+00:43:53,550 --> 00:43:56,830
+شوية بالـ methylene blue وشوية بالـ eosin، عشان هيك
+
+563
+00:43:56,830 --> 00:44:00,130
+بتكون لونها دائمًا، حبيبات الـ neutrophil بيكون لونها
+
+564
+00:44:00,130 --> 00:44:04,630
+pink، ما أخذة الـ يعني خليط من اللون الـ blue
+
+565
+00:44:04,630 --> 00:44:08,950
+مع اللون الـ orange للـ red، هذا فبتعطيني اللون الـ
+
+566
+00:44:08,950 --> 00:44:13,510
+pink. طبعًا لازم أنا أستخدم buffer، phosphate buffer،
+
+567
+00:44:13,510 --> 00:44:18,050
+أضيفه على الصبغة، علشان يؤدي إلى تأيينها، الشق
+
+568
+00:44:18,050 --> 00:44:22,350
+القاعدي تبعها ياخد الشحنة الموجبة، فيصبغ الأجزاء
+
+569
+00:44:22,350 --> 00:44:29,230
+السالبة، والجزء الحامضي ياخد الشحنة السالبة، فيصبغ الـ
+
+570
+00:44:29,230 --> 00:44:36,170
+الأجزاء الموجبة في الخلية. طبعًا هنا، ميعطيني طريقة
+
+571
+00:44:36,170 --> 00:44:40,330
+صباغة بالـ Leishman’s stain. طبعًا، حاكي إنّه بحضر الـ smear،
+
+572
+00:44:40,330 --> 00:44:45,330
+بخليه air-dry، على طول بعد الـ air-dry بحط الـ stain،
+
+573
+00:44:45,330 --> 00:44:50,150
+ليش؟ لأن الـ stain هي منها fixative ومنها صبغة في نفس
+
+574
+00:44:50,150 --> 00:44:56,210
+الوقت، وصبغها، بعد هيك بخليها تجف، وطبعًا بحط الصبغة،
+
+575
+00:44:56,210 --> 00:44:59,250
+بضيف الـ buffer، الـ buffer بيخلي الصبغة تتأين وتصبغ،
+
+576
+00:44:59,250 --> 00:45:03,810
+بتركها من عشر لـ خمس عشرة دقيقة، بغسلها، بخليها تجف،
+
+577
+00:45:03,810 --> 00:45:08,770
+بعدين بشوفها على الـ microscope. تمام؟ فإذا إحنا
+
+578
+00:45:08,770 --> 00:45:12,410
+هناخد طريقة ثانية، لأنّه هنستخدم الـ Giemsa، وهشرح لكم
+
+579
+00:45:12,410 --> 00:45:18,800
+الطريقة بالمختبر. طيب، إيش الألوان اللي هتأخذها
+
+580
+00:45:18,800 --> 00:45:22,640
+أجزاء الخلايا؟ طبعًا إحنا حكينا أنوية الخلايا موجود
+
+581
+00:45:22,640 --> 00:45:28,560
+فيها DNA وRNA وnuclear proteins، فهتأخذ الصبغة
+
+582
+00:45:28,560 --> 00:45:32,320
+القاعدية، اللي هي الـ methylene blue أو الـ azur،
+
+583
+00:45:32,320 --> 00:45:36,380
+فهتكون أخذة اللون الـ purple، حكيت لكم هي إمّا violet
+
+584
+00:45:36,380 --> 00:45:40,470
+أو الـ blue، فهتكون أخذة اللون الـ purple. الـ
+
+585
+00:45:40,470 --> 00:45:44,010
+cytoplasm، الـ cytoplasmal erythrocyte فيه
+
+586
+00:45:44,010 --> 00:45:47,110
+هيموغلوبين، خلايا الدم الحمراء، الـ cytoplasm تبعها
+
+587
+00:45:47,110 --> 00:45:52,110
+مليان هيموغلوبين، فهتأخذ الـ eosin، فهي ينصبغ بالـ
+
+588
+00:45:52,110 --> 00:45:58,590
+eosin وتأخذ اللون الـ red. تمام، الـ neutrophil الـ
+
+589
+00:45:58,590 --> 00:46:03,230
+cytoplasm تبعها موجود فيه حبيبات، والحبيبات هذه
+
+590
+00:46:03,230 --> 00:46:07,550
+متعادلة، فهتأخذ الـ blue بقدر ما، يعني الـ methylene
+
+591
+00:46:07,550 --> 00:46:15,000
+blue بقدر ما هتنصبغ بالـ eosin Y أو الـ eosin
+
+592
+00:46:15,000 --> 00:46:19,860
+B، فبالتالي هيكون لونها ما بين الـ orange للـ pink.
+
+593
+00:46:19,860 --> 00:46:23,800
+طبعًا الـ lymphocytes، الـ cytoplasm تبعها هيكون blue،
+
+594
+00:46:23,800 --> 00:46:28,440
+يعني منصبغ بالـ methylene blue. تمام، الـ monocytes هيكون
+
+595
+00:46:28,440 --> 00:46:31,660
+برضه كمان الـ cytoplasm تبعها blue، والـ basophils
+
+596
+00:46:31,660 --> 00:46:35,160
+هيكون الـ cytoplasm تبعها blue. الحبيبات، الحبيبات للـ
+
+597
+00:46:35,160 --> 00:46:38,480
+neutrophils، حكينا هيكون لونها fine purple أو يعني
+
+598
+00:46:38,480 --> 00:46:43,140
+موف فاتح أو pink، حكينا ليش؟ والـ eosinophilic
+
+599
+00:46:43,140 --> 00:46:47,320
+حبيباتها هتكون أخذة صبغة الـ eosin، اللي هي red لـ
+
+600
+00:46:47,320 --> 00:46:52,060
+orange. الـ basophil أخذة صبغة الـ basic، اللي هي لون
+
+601
+00:46:52,060 --> 00:46:58,360
+الـ methylene blue، هتكون أخذة
+
+602
+00:46:58,360 --> 00:47:01,680
+اللون الـ blue أو الـ purple، أو من كثر ما هي
+
+603
+00:47:01,680 --> 00:47:05,320
+basophilic هتكون أخذة الـ purple كثير لدرجة أنها صارت
+
+604
+00:47:05,320 --> 00:47:11,060
+black، black. تمام؟ الـ monocytes، طبعًا اللي
+
+605
+00:47:11,060 --> 00:47:14,920
+فيها granules، وما تأخذ fine reddish، أو azurophil
+
+606
+00:47:14,920 --> 00:47:18,950
+granules. طب، طب لحظة. إحنا مش صنّفنا إنه الـ monocytes والـ
+
+607
+00:47:18,950 --> 00:47:22,370
+lymphocytes هي عبارة عن agranulocytes، يعني ما فيش
+
+608
+00:47:22,370 --> 00:47:26,690
+فيها حبيبات؟ شوفوا هو لما صنّفوهم، حكوا إنه الـ white
+
+609
+00:47:26,690 --> 00:47:31,010
+blood cells نوعين، granulocytes، اللي هم الـ
+
+610
+00:47:31,010 --> 00:47:
+
+667
+00:51:53,490 --> 00:51:57,410
+cytoplasm تبعها في حبيباتها طيب نجي للـ agranulocyte
+
+668
+00:51:57,410 --> 00:52:00,190
+اللي هي الخلايا الغير محببة أو على الأقل الـ
+
+669
+00:52:00,190 --> 00:52:05,830
+monocyte إحنا حكينا بنمشيها على أنها agranulocytes
+
+670
+00:52:05,830 --> 00:52:09,410
+يولوسايت ليش؟ لأن الحبيبات تبعها بتكون غير واضحة إلا
+
+671
+00:52:09,410 --> 00:52:11,610
+لازم أشوفها على electron microscope يعني على
+
+672
+00:52:11,610 --> 00:52:15,790
+microscope  ذو تكبير عالٍ جدًا تمام، يعني في الـ
+
+673
+00:52:15,790 --> 00:52:18,050
+light microscope  الميكروسكوب العادي اللي إحنا
+
+674
+00:52:18,050 --> 00:52:22,030
+بنستخدمه، صعب أشوف حبيبات الـ monocyte عشان هيك
+
+675
+00:52:22,030 --> 00:52:25,930
+ممكن نحكي عنها مجازًا أنها agranulocytes لكن
+
+676
+00:52:25,930 --> 00:52:30,500
+اللي بدي إياكم تعرفوه أنه هي فيها حبيبات، طيب ما
+
+677
+00:52:30,500 --> 00:52:33,660
+علي الـ lymphocyte هي عبارة عن خلية صغيرة small
+
+678
+00:52:33,660 --> 00:52:40,540
+white blood cells والنواة تبعها round or oval تمام
+
+679
+00:52:40,540 --> 00:52:46,340
+بتكون إما دائرية أو بيضاوية، يعني مقاربة لحجم الـ
+
+680
+00:52:46,340 --> 00:52:50,130
+RBCs، يعني لو أنتِ شفتِ خلية وشكيتي أنها lymphocyte
+
+681
+00:52:50,130 --> 00:52:53,010
+على طول اطلعي على النواة تبعها واطلعي على الـ RBCs
+
+682
+00:52:53,010 --> 00:52:58,750
+اللي جنبها، إذا لقيتهم متقاربين في الحجم معناها
+
+683
+00:52:58,750 --> 00:53:02,050
+أنها هي على الأغلب lymphocyte، الـ cytoplasm تبعها
+
+684
+00:53:02,050 --> 00:53:09,650
+بيكون أزرق ورمادي، وبتكون طبعًا النواة تشغل حجمًا
+
+685
+00:53:09,650 --> 00:53:14,320
+كبيرًا من الـ cytoplasm لدرجة أنه بس عندي حلقة رفيعة
+
+686
+00:53:14,320 --> 00:53:17,180
+من الـ cytoplasm، شايفين هنا حلقة رفيعة من الـ
+
+687
+00:53:17,180 --> 00:53:21,060
+cytoplasm هي اللي بتكون مبينة، بنسميها thin rim of
+
+688
+00:53:21,060 --> 00:53:28,360
+cytoplasm، إذا نيجي هنا ثاني الـ lymphocyte
+
+689
+00:53:28,360 --> 00:53:36,780
+lymphocyte has a thin rim of
+
+690
+00:53:39,550 --> 00:53:40,590
+cytoplasm
+
+691
+00:53:43,420 --> 00:53:47,700
+طبعًا، فإذا الـ lymphocyte لها هيك زي حلقة بسيطة من
+
+692
+00:53:47,700 --> 00:53:52,280
+الـ cytoplasm بتكون موجودة لأن النواة محتلة معظم
+
+693
+00:53:52,280 --> 00:53:56,380
+حجم الخلية، طبعًا زي ما أنتم شايفين هنا هذه اللي هي
+
+694
+00:53:56,380 --> 00:53:59,480
+الـ lymphocyte، طب نجي للـ monocyte، الـ monocyte
+
+695
+00:53:59,480 --> 00:54:03,420
+غالبا بتكون النواة تبعها kidney .. sorry، النواة تبعها
+
+696
+00:54:03,420 --> 00:54:06,960
+kidney-shaped، طبعًا زي شكل الكلية زي شكل الـ kidney
+
+697
+00:54:06,960 --> 00:54:09,640
+shape أو حدوة الفرس، ولكن
+
+698
+00:54:11,840 --> 00:54:17,380
+طيب، ولكن الـ .. الـ .. ممكن أنا أشوف monocyte
+
+699
+00:54:17,380 --> 00:54:21,020
+النواة تبعها شكلها irregular يعني غير منتظم برضه كمان
+
+700
+00:54:21,020 --> 00:54:24,880
+بيكون شكلها غير منتظم، بعرفها أنها عبارة عن خلية
+
+701
+00:54:24,880 --> 00:54:29,440
+كبيرة جدًا والنواة تبعها غير منتظمة والـ
+
+702
+00:54:29,440 --> 00:54:35,130
+cytoplasm تبعها فيه زي غبش هنا نتيجة وجود  أيه
+
+703
+00:54:35,130 --> 00:54:38,590
+مش حاكية هنا الـ azurophilic granules اللي هي غير واضحة
+
+704
+00:54:38,590 --> 00:54:43,130
+على الـ light microscope طبعًا، فعشانها غير واضحة
+
+705
+00:54:43,130 --> 00:54:46,990
+بتكون الـ cytoplasm غير نقي أو غير pure زي مثلًا هنا
+
+706
+00:54:46,990 --> 00:54:51,630
+طبعًا، فبيكون زي غبش فيه، هذا بسبب وجود الـ
+
+707
+00:54:51,630 --> 00:54:56,660
+azurophilic granules، طيب، طب أنا كيف بدي أفرّق ما
+
+708
+00:54:56,660 --> 00:55:00,500
+بين مثلًا الـ band  وما .. ما بين الـ monocyte؟ أول
+
+709
+00:55:00,500 --> 00:55:05,320
+حاجة في حال .. في حالة الـ band الحبيبات إيه بتفرج؟
+
+710
+00:55:05,320 --> 00:55:07,900
+يعني لو كانت حبيباتها وردية فهي band  band
+
+711
+00:55:07,900 --> 00:55:12,160
+neutrophil، لو كانت حبيباتها حمراء معناها هي band
+
+712
+00:55:12,160 --> 00:55:16,700
+eosinophil، ولو كانت حبيباتها بنفسجية غامقة لا زرقاء لا
+
+713
+00:55:16,700 --> 00:55:21,880
+سوداء فمعناها هي band basophil، تمام؟ طيب، هذه شغلة
+
+714
+00:55:21,880 --> 00:55:25,760
+ثانية، لكن أنه أنتم زي .. شايفين النواة نفس الشكل
+
+715
+00:55:25,760 --> 00:55:31,020
+طيب، هلأ لو أنا جيت سكرت الـ .. النواة و .. وكملتها
+
+716
+00:55:31,020 --> 00:55:36,440
+بشكل دائري، تمام؟ ورسمت خط وهمي من نهاية النواة
+
+717
+00:55:36,440 --> 00:55:43,110
+إلى الـ .. الـ .. لهنا، تمام؟ إذا كان الخط هذا هو
+
+718
+00:55:43,110 --> 00:55:48,970
+يعني أكبر من .. من نصف قطر الدائرة اللي أنا سكرتها
+
+719
+00:55:48,970 --> 00:55:54,130
+معناها دي band، تمام؟ يعني مثلًا بسكر وبحدد المركز،
+
+720
+00:55:54,130 --> 00:55:57,950
+نقطة المركز، وبَرسم، لو المسافة أكثر من نصف القطر
+
+721
+00:55:57,950 --> 00:56:03,570
+معناها أنه دي band، تمام؟ لكن لو أنا هنا جيت سكرت و
+
+722
+00:56:03,570 --> 00:56:09,010
+رسمت خط، تمام وعلى اعتبار أنه مثلًا نصف القطر هنا فـ
+
+723
+00:56:09,010 --> 00:56:13,490
+.. أو المركز هنا، هيكون فالمسافة اللي مفتوحة هذه
+
+724
+00:56:13,490 --> 00:56:17,450
+الفرزة هي عبارة عن مساوية أو أقل من نصف .. لا نصف
+
+725
+00:56:17,450 --> 00:56:22,690
+القطر فمعناها أنه هذه خلية monocyte أو نواة
+
+726
+00:56:22,690 --> 00:56:24,190
+monocyte مش نواة band
+
+727
+00:56:27,180 --> 00:56:31,120
+طيب، إذا هيك بتكون عرفنا على أشكال الخلايا، طب
+
+728
+00:56:31,120 --> 00:56:34,860
+البلاتلت، إحنا حكينا أنه البلاتلت هي فتافيت من
+
+729
+00:56:34,860 --> 00:56:39,680
+خلية الـ megakaryocyte صح؟ تمام، فالـ megakaryocyte
+
+730
+00:56:39,680 --> 00:56:43,400
+هي خلية كبيرة موجودة في bone marrow لما بيتفتت الـ
+
+731
+00:56:43,400 --> 00:56:46,160
+cytoplasm تبعها وبيطلع على الـ circulation بيطلع على
+
+732
+00:56:46,160 --> 00:56:52,460
+هيئة البلاتلت، اللي هي الصفائح الدموية، تمام؟ فهذه
+
+733
+00:56:52,460 --> 00:56:56,700
+الحبيبات أو النقاط اللي لونها بنفسجي هذه عبارة عن
+
+734
+00:56:56,700 --> 00:57:00,980
+بلاتلت، هي هنا هذه بلاتلت، تمام، هذه بلاتلت، هذه
+
+735
+00:57:00,980 --> 00:57:05,520
+بنسميها بلاتلت، اللي هنا تكون موجودة ما بين الـ RBCs
+
+736
+00:57:05,520 --> 00:57:09,080
+فبنسميها بلاتلت، هذا تجمع من
+
+737
+00:57:09,080 --> 00:57:13,760
+البلاتلت، طيب الـ RBCs اللي هو الـ red blood cells زي
+
+738
+00:57:13,760 --> 00:57:19,950
+ما أنتم شايفين، خلايا شكلها قرصي، تمام، وممكن
+
+739
+00:57:19,950 --> 00:57:23,630
+تكون أخذت شكل بيضاوي أو شيء من الضغط، لكن
+
+740
+00:57:23,630 --> 00:57:26,950
+هي شكلها قرصي، أنا لو جيت طلعت عليها، حكينا الـ RBCs
+
+741
+00:57:28,050 --> 00:57:32,590
+لما أجي أطلع عليها من الـ .. الـ .. الـ .. الجهة
+
+742
+00:57:32,590 --> 00:57:36,250
+العلوية بشوفها قرصية، لكن لما أجي أطلع عليها
+
+743
+00:57:36,250 --> 00:57:39,690
+من الجانب بشوفها مقعرة، طبعًا، فإذا إني نجي
+
+744
+00:57:39,690 --> 00:57:43,190
+أطلع عليها من فوق قرصية زي القرص، طبعًا زي
+
+745
+00:57:43,190 --> 00:57:47,570
+القرص، وفيها الهيموجلوبين، الـ
+
+746
+00:57:47,570 --> 00:57:50,990
+الهيموجلوبين بيحتل أكثر من ثلثي حجمها، يعني أكثر من 60%
+
+747
+00:57:50,990 --> 00:57:55,270
+من حجمها، وفي في الوسط منطقة فاضية، هذه المنطقة
+
+748
+00:57:55,270 --> 00:57:59,530
+الفاضية بسميها الـ central pallor، تمام شحوب، تمام
+
+749
+00:57:59,530 --> 00:58:03,170
+central pallor، فهذا الـ central pallor بيحتل طولًا
+
+750
+00:58:03,170 --> 00:58:08,330
+الخلية، central pallor
+
+751
+00:58:08,330 --> 00:58:09,310
+of
+
+752
+00:58:11,880 --> 00:58:17,320
+RBC's، طبعًا؟ شوفوا إيش يعني؟ central pallor
+
+753
+00:58:17,320 --> 00:58:22,700
+الشحوب، كلمة pallor شحوب طبعًا؟ of RBC's، طيب ليش أنا
+
+754
+00:58:22,700 --> 00:58:26,820
+سميتها central pallor of RBC's؟ طلعوا معي، إحنا هلأ
+
+755
+00:58:26,820 --> 00:58:29,280
+جينا لما نحكي عن شخص شاحب، إيش يعني؟ والله بنقول والله
+
+756
+00:58:29,280 --> 00:58:34,180
+دمه ضعيف وهو شاحب، تمام؟ يعني ما في عنده
+
+757
+00:58:34,180 --> 00:58:37,880
+هيموجلوبين كافي، فنفس الشيء المنطقة هذه ما في فيها
+
+758
+00:58:37,880 --> 00:58:41,660
+هيموجلوبين، فبالتالي بسميها شاحبة، المنطقة الشاحبة
+
+759
+00:58:41,660 --> 00:58:45,300
+من الخلية لأنها منطقة ما في فيها هيموجلوبين ومش
+
+760
+00:58:45,300 --> 00:58:49,020
+ماخدة لون الهيموجلوبين، تمام؟ لكن لما نحكي عن شخص
+
+761
+00:58:49,020 --> 00:58:53,580
+والله أنه متورد، ليش؟ لأنه .. لأنه مثلًا الدم بيسير
+
+762
+00:58:53,580 --> 00:58:58,450
+عنده نتيجة وجود هيموجلوبين، تمام؟ فإذا مرتبط دائمًا
+
+763
+00:58:58,450 --> 00:59:01,670
+مصطلح الشحوب في أنه ما في عنده هيموجلوبين أو أنه
+
+764
+00:59:01,670 --> 00:59:05,810
+عنده دم فقير أو كذا، فنفس الشيء أن هذه المنطقة
+
+765
+00:59:05,810 --> 00:59:10,350
+سميت بالـ pallor، بالمنطقة الشاحبة المركزية اللي
+
+766
+00:59:10,350 --> 00:59:17,050
+بتحتل طول الخلية، نظرًا لأنها فراغ مش
+
+767
+00:59:17,050 --> 00:59:21,080
+موجود فيها هيموجلوبين، طب إيش موجود فيها؟ موجود فيها
+
+768
+00:59:21,080 --> 00:59:25,060
+cytoplasm ومكونات أخرى للخلية، يعني إنزيمات
+
+769
+00:59:25,060 --> 00:59:30,400
+وبروتينات أخرى بتلزم الخلية في حياتها اللي هو خلال
+
+770
+00:59:30,400 --> 00:59:32,760
+الـ 120 يوم رحلة حياتها
+
+771
+00:59:37,700 --> 00:59:44,760
+طيب، أنا هيك سبقت الـ .. الـ .. الشريحة، واتعرفت كيف
+
+772
+00:59:44,760 --> 00:59:49,740
+يعني .. أصبح صار عندي فكرة على الـ .. الخلايا و
+
+773
+00:59:49,740 --> 00:59:55,820
+أجزائها، وكيف هيكون شكلها بعد ما تاخد الصبغة و ..
+
+774
+00:59:55,820 --> 01:00:00,030
+هلأ بدي أنا أشوفها تحت الـ microscope، بدي أجيب الـ
+
+775
+01:00:00,030 --> 01:00:06,130
+field على 10x، بعدين أحوّل على 100x، طبعًا الـ 100x أنا
+
+776
+01:00:06,130 --> 01:00:10,430
+محتاجة أني أحط قطرة زيت علشان أشوف بشكل واضح
+
+777
+01:00:10,430 --> 01:00:14,770
+طبعًا، طيب، ممكن أعمل differential، إيش يعني
+
+778
+01:00:14,770 --> 01:00:18,510
+differential؟ يعني أشوف أعدّ 100 خلية من الـ white
+
+779
+01:00:18,510 --> 01:00:24,390
+blood cells، وكل ما أعد خلية بعرف بجيب نوعها، طبعًا أو
+
+780
+01:00:24,390 --> 01:00:28,080
+يعني بحدد نوعها، في النهاية بعد ما أكون عديت 100
+
+781
+01:00:28,080 --> 01:00:31,820
+خلية، بدي أشوف من المئة هدول كم كانوا monocyte،
+
+782
+01:00:31,820 --> 01:00:34,200
+كم كانوا neutrophil، كم كانوا eosinophil أو
+
+783
+01:00:34,200 --> 01:00:38,520
+basophil، أو يعني الخلايا الأخرى، فبالتالي هيك بكون
+
+784
+01:00:38,520 --> 01:00:42,220
+أنا عملت differential، جبت نسبة كل نوع من أنواع
+
+785
+01:00:42,220 --> 01:00:46,740
+الـ white blood cells من أصل 100 خلية أنا عدّيتهم، تمام؟
+
+786
+01:00:47,580 --> 01:00:52,400
+طيب، بس أنتم هالشيء مش .. يعني في المرحلة الحالية
+
+787
+01:00:52,400 --> 01:00:55,120
+مش مطلوب منكم تعملوا differential، هتعملوا إن شاء
+
+788
+01:00:55,120 --> 01:00:59,620
+الله بس تروحوا على الـ hematology، لكن المطلوب منكم
+
+789
+01:00:59,620 --> 01:01:02,720
+حاليًا أنكم تكونوا بتعرفوا تجيبوا الـ field، بتعرفوا
+
+790
+01:01:02,720 --> 01:01:07,500
+إيش الأساسيات الميكروسكوب وكيف تستكشف الشريحة، كيف
+
+791
+01:01:07,500 --> 01:01:10,720
+تتعرفوا على شكل الخلايا، يعني هذه RBCs هذه بلاتلت
+
+792
+01:01:10,720 --> 01:01:13,140
+هذه lymphocyte، monocyte، eosinophil،
+
+793
+01:01:13,360 --> 01:01:19,450
+neutrophil، basophil وهكذا، تمام؟ طيب، أنا بدي أجيب
+
+794
+01:01:19,450 --> 01:01:23,690
+الـ field على عشرة، طبعًا اللي هي مثلًا الشريحة تبعيتي
+
+795
+01:01:23,690 --> 01:01:28,050
+وعليها الـ blood film اللي أنا فردته، طبعًا لازم أنا
+
+796
+01:01:28,050 --> 01:01:31,410
+لما أجي أركز معانا مركز الشريحة، يعني بدي أحط
+
+797
+01:01:31,410 --> 01:01:35,050
+المنطقة القراءة اللي بدي أقرأها بدي يكون تحتها الـ
+
+798
+01:01:35,050 --> 01:01:40,870
+condenser وفوقها مباشرة اللي هي العدسة، طبعًا؟ طيب
+
+799
+01:01:40,870 --> 01:01:47,030
+فالمنطقة اللي بتقرأ هي منطقة الـ .. الـ .. الـ .. الـ
+
+800
+01:01:47,030 --> 01:01:53,030
+.. يعني الطرف الرفيع أو المنطقة الرفيعة في الـ
+
+801
+01:01:53,030 --> 01:01:58,070
+blood smear، تمام؟ هذه هي المنطقة اللي أنا بقراها
+
+802
+01:01:58,070 --> 01:02:01,710
+فإذا هذه المنطقة اللي بدي يكون تحتها الـ condenser
+
+803
+01:02:01,710 --> 01:02:08,400
+وفوقها العدسة، طيب، وإذا بـ .. بحط عدسة عشرة on وبـ
+
+804
+01:02:08,400 --> 01:02:12,520
+ببدأ أرفع الـ stage شوي شوي لحد ما أشوف الخلايا، بعد
+
+805
+01:02:12,520 --> 01:02:17,720
+هيك بروح بدور على منطقة في .. يعني على .. يعني ..
+
+806
+01:02:17,720 --> 01:02:21,920
+مكان، في هذه المنطقة أنه يكون الخلايا evenly
+
+807
+01:02:21,920 --> 01:02:25,900
+distributed، إيش يعني evenly distributed؟ يعني أنه
+
+808
+01:02:25,900 --> 01:02:32,900
+تكون الخلايا موزعة بشكل منيح، وتكون متباعدة شوي، ومش
+
+809
+01:02:32,900 --> 01:02:38,020
+يعني مش متراكبة على بعضها البعض، وما في زحمة، و
+
+810
+01:02:38,020 --> 01:02:42,720
+تكون عشان أنا أتعرف على شكل الخلايا بشكل واضح و
+
+811
+01:02:42,720 --> 01:02:50,670
+أصنفها بشكل دقيق، تمام؟ طيب، وبقيم من خلالها الـ RBCs
+
+812
+01:02:50,670 --> 01:02:54,990
+distribution، شكلها عام، information، مش عاملة
+
+813
+01:02:54,990 --> 01:02:59,870
+role، الـ .. الخلايا، الهيموجلوبين تبعها كويس ولا
+
+814
+01:02:59,870 --> 01:03:03,250
+فاضي، إذا طبعًا كيف بـ .. بـ .. بحكم إذا الـ central
+
+815
+01:03:03,250 --> 01:03:06,170
+pallor هذا محتل أكثر من طول الخلية، معناها أنها
+
+816
+01:03:06,170 --> 01:03:08,490
+خلية شاحبة وما في فيها هيموجلوبين، يعني الشخص عنده
+
+817
+01:03:08,490 --> 01:03:14,250
+anemia، طيب
+
+818
+01:03:16,450 --> 01:03:21,910
+وبعد هيك بحط قطرة زيت وبحول على المية، وما تنسوش أنه
+
+819
+01:03:21,910 --> 01:03:26,050
+إحنا بنفتح القرص في هذه اللحظة، علشان أنا بدي إضاءة
+
+820
+01:03:26,050 --> 01:03:31,190
+عالية، وببدأ أفحص العينة بشكل systematic، بشكل زي
+
+821
+01:03:31,190 --> 01:03:34,710
+زاج، طبعًا، إحنا كمان حكينا أنه العد برضه كمان بيكون
+
+822
+01:03:34,710 --> 01:03:38,110
+بشكل زي زاج، إذا بروح من الطرف اللي أنا ببدأ وبـ
+
+823
+01:03:38,110 --> 01:03:44,010
+بفوت لجوه بهذا الشكل، طبعًا زي زاج، وبكون أنا بعد
+
+824
+01:03:44,010 --> 01:03:48,990
+هيك استكشفت الخلية، طبعًا أو استكشفت أشكال الخلايا و
+
+825
+01:03:48,990 --> 01:03:52,830
+اتعرفت عليها، الـ .. هذه الـ basophil شايفين؟ two
+
+826
+01:03:52,830 --> 01:03:57,590
+lobes، طبعًا والحبيبات كبيرة، بتغطي النواة، أو يعني
+
+827
+01:03:57,590 --> 01:04:00,910
+موجودة، أحيانًا بتكون بتغطي كل الـ .. كل النواة
+
+828
+01:04:00,910 --> 01:04:04,850
+ما .. يعني ما بيبين تفاصيل النواة منها، الـ
+
+829
+01:04:04,850 --> 01:04:08,390
+neutrophil، حبيباتها ناعمة ولونها وردي، وهذه شايفين
+
+830
+01:04:08,390 --> 01:04:09,370
+هذه أربعة lobes
+
+831
+01:04:12,390 --> 01:04:16,210
+ممكن يكونوا خمسة، يعني ممكن يكون هذا الخامس هنا
+
+832
+01:04:16,210 --> 01:04:21,470
+eosinophil، هذه ثلاثة lobes وحبيباتها لونها أحمر، هذه
+
+833
+01:04:21,470 --> 01:04:26,890
+lymphocyte، طبعًا اللي حكينا أنه في عندي thin rim of
+
+834
+01:04:26,890 --> 01:04:30,15
+
+889
+01:09:31,660 --> 01:09:34,680
+parasite ضد الطفيليات و أيضا لها دور في ال
+
+890
+01:09:34,680 --> 01:09:38,320
+allergy و الـ basophil كمان لها دور في الـ allergy
+
+891
+01:09:38,320 --> 01:09:42,110
+و في الـ antigen representation، يعني في تقديم الـ
+
+892
+01:09:42,110 --> 01:09:47,210
+antigen للخلايا الأخرى عشان انها تقتله، تمام؟ هذا
+
+893
+01:09:47,210 --> 01:09:52,410
+وظائف الخلايا أو حسب يعني .. أو تخصصها في الدفاع
+
+894
+01:09:52,410 --> 01:09:57,730
+عن جسم الإنسان. طيب، لو كان في عندي زيادة في الـ
+
+895
+01:09:57,730 --> 01:10:02,050
+lymphocyte، إيش بسميها هذا المصطلح؟ بسميه
+
+896
+01:10:02,050 --> 01:10:10,000
+lymphocytosis، تمام. lymphocytosis. وإذا كانت في
+
+897
+01:10:10,000 --> 01:10:15,160
+عندي طبعا قلة في الـ lymphocyte أو نقص في عدد الـ
+
+898
+01:10:15,160 --> 01:10:20,900
+lymphocyte، فمعناها هذا بسميه lympho
+
+899
+01:10:20,900 --> 01:10:29,400
+lymphocytopenia
+
+900
+01:10:29,400 --> 01:10:35,450
+lymphocytopenia. على كل حال، ليكوبينيا حكينا الليكوبينيا
+
+901
+01:10:35,450 --> 01:10:39,310
+يعني قلة في الـ white blood cells ككل، والـ .. والـ ..
+
+902
+01:10:39,310 --> 01:10:42,990
+ليكو سايتوسز يعني زيادة في الـ white blood cells ككل، و
+
+903
+01:10:42,990 --> 01:10:49,110
+هاد .. هاد الشيء ذكرناه في محاضرة الـ .. الـ white
+
+904
+01:10:49,110 --> 01:10:53,010
+blood cells، تمام؟ وذكرناه أيضا في .. في فحص الـ
+
+905
+01:10:53,010 --> 01:10:57,890
+hemoglobin، تمام،
+
+906
+01:10:57,890 --> 01:11:02,650
+monocytosis. نفس فكرة الـ lymphocyte، ما هي اصلا كلمة
+
+907
+01:11:02,650 --> 01:11:06,570
+lymphocyte تنتهي بـ cyte، والـ monocyte تنتهي بـ cyte
+
+908
+01:11:06,570 --> 01:11:12,090
+فعشان هما بينتهوا زي بعض، فبيكون
+
+909
+01:11:12,090 --> 01:11:16,670
+المصطلح اللي بيعبر عن مجموعهما أو عن زيادتهما أو
+
+910
+01:11:16,670 --> 01:11:20,230
+نقصانهما نفس الشيء. مثلا زيادة الـ lymphocyte
+
+911
+01:11:20,230 --> 01:11:26,110
+lymphocytosis، زيادة الـ monocyte monocytosis.
+
+912
+01:11:28,340 --> 01:11:38,620
+وطبعا نقص الـ monocyte هيكون monocytopenia.
+
+913
+01:11:38,620 --> 01:11:45,160
+.. monocytopenia. طبعا. طيب، الـ .. الـ neutrophil في
+
+914
+01:11:45,160 --> 01:11:51,660
+الزيادة neutrophilia.
+
+915
+01:11:51,660 --> 01:11:56,660
+.. طبعا neutrophilia، ونقصانها
+
+916
+01:12:04,910 --> 01:12:08,250
+neutropenia. نفس الشيء، الـ eosinophil زيادتها
+
+917
+01:12:08,250 --> 01:12:14,370
+eosinophilia، ونقصانها eosinopenia. basophilia,
+
+918
+01:12:14,770 --> 01:12:20,550
+basocytopenia. طبعا، فبالتالي لازم هذه الشغلات أنتم
+
+919
+01:12:20,550 --> 01:12:22,890
+تكونوا بتعرفوها، أو المصطلحات تكونوا بتعرفوها، و
+
+920
+01:12:22,890 --> 01:12:25,570
+تكونوا بتعرفوا إيش فكرة الـ differential يعني، طبعا
+
+921
+01:12:25,570 --> 01:12:30,030
+لكن أنه فعليا تفاصيل الـ differential count إيش مطلوب منكم
+
+922
+01:12:30,030 --> 01:12:35,010
+أو كيف أنه تعملوا هذا، تاخدوا في الـ hematology
+
+923
+01:12:35,010 --> 01:12:43,870
+تمام؟ بهيك بنكون وصلنا لنهاية محاضرتنا لليوم، إن
+
+924
+01:12:43,870 --> 01:12:46,570
+شاء الله تكون واضحة وسهلة بالنسبة لكم، ويعطيكم
+
+925
+01:12:46,570 --> 01:12:47,390
+ألف عافية
diff --git a/PL9fwy3NUQKwY9jl8xw-bWkVZkWcGGyspd/ELhdSBMOyuo_postprocess.srt b/PL9fwy3NUQKwY9jl8xw-bWkVZkWcGGyspd/ELhdSBMOyuo_postprocess.srt
new file mode 100644
index 0000000000000000000000000000000000000000..e8677e61d6ccfd2e22a0c7653adea71ac711ab14
--- /dev/null
+++ b/PL9fwy3NUQKwY9jl8xw-bWkVZkWcGGyspd/ELhdSBMOyuo_postprocess.srt
@@ -0,0 +1,3700 @@
+1
+00:00:11,190 --> 00:00:14,530
+السلام عليكم ورحمة الله وبركاته طالبات العزيزات
+
+2
+00:00:14,530 --> 00:00:18,470
+اليوم ان شاء الله راح نتحدث عن blood smear
+
+3
+00:00:18,470 --> 00:00:22,770
+examination and preparation تمام؟ هو blood smear
+
+4
+00:00:22,770 --> 00:00:27,750
+preparation and examination المفروض اليوم نتعلم شو
+
+5
+00:00:27,750 --> 00:00:31,970
+يعني مسحة الدم؟ كيف أنا بحضر هاي المسحة؟ وكيف ممكن
+
+6
+00:00:31,970 --> 00:00:35,780
+أنا أسبخها و أشوفها تحت ال microscope؟لحتى أستكشف
+
+7
+00:00:35,780 --> 00:00:41,800
+خلايا الدم في البداية ال blood smear هو عبارة عن
+
+8
+00:00:41,800 --> 00:00:46,940
+مسحة أو فرد للدم على الشريحة تمام؟ فرد لعينة الدم
+
+9
+00:00:46,940 --> 00:00:50,940
+على الشريحة بحيث اني انا بإمكاني ان انا اثبت هذه
+
+10
+00:00:50,940 --> 00:00:55,560
+العينة على الشريحة و من ثم اصبغها و بعد هيك اشوف
+
+11
+00:00:55,560 --> 00:01:00,180
+اتعرف على أشكال خلايا الدم ليش طيب انا بدى اتعرف
+
+12
+00:01:00,180 --> 00:01:04,620
+على ال morphology؟of RBCs أو .. أو ال morphology
+
+13
+00:01:04,620 --> 00:01:10,480
+of white BCs لإنه أشكال خلايا الدم سواء كانت white
+
+14
+00:01:10,480 --> 00:01:16,580
+BCs أو RBCs بتكون ذات دلالة أو بتساعدنا في ال
+
+15
+00:01:16,580 --> 00:01:23,210
+diagnosis في تشخيص بعد الأمراض تمام؟مثلا إذا كانت
+
+16
+00:01:23,210 --> 00:01:27,410
+.. إذا كان الشخص عنده anemia فأنا بلاحظ إن ال RBC
+
+17
+00:01:27,410 --> 00:01:31,670
+بتكون فاضية أو مافيش فيها لون أحمر كتير تمام؟ إذا
+
+18
+00:01:31,670 --> 00:01:36,010
+كان مثلا في عندي white bc كتيرة أو ال white bc هذي
+
+19
+00:01:36,010 --> 00:01:39,610
+immature معناها بشك أن الشخص ممكن يكون مصاب
+
+20
+00:01:39,610 --> 00:01:46,410
+بلوكيميا حتى يعني زيادة نوع واحد من الخلايا هذا
+
+21
+00:01:46,410 --> 00:01:53,470
+أيضا بيرتبط ب ..بتشخيص معين إذا أنا لازم أتعرف على
+
+22
+00:01:53,470 --> 00:01:57,810
+أشكال الخلايا هاي و أتعرف ممكن كمان إن أنا أعد أو
+
+23
+00:01:57,810 --> 00:02:03,250
+أعمل differential لإلها لحتى أتمكن من المساعدة في
+
+24
+00:02:03,250 --> 00:02:08,410
+التشخيص تمام ال blood film هذا دايما بيكون مرافق
+
+25
+00:02:08,410 --> 00:02:11,630
+لل CBC يعني إذا أنا عملت CBC أو عملت مثلا ال
+
+26
+00:02:11,630 --> 00:02:17,090
+homoglobin test عملت hematocrit حسبة ال MCV و ال
+
+27
+00:02:17,090 --> 00:02:26,370
+.. ال ..قانون التلاتة مثلا الأشياء هاي فطبعا لازم
+
+28
+00:02:26,370 --> 00:02:31,790
+معها أكون عملت برضه كمان blood film there are
+
+29
+00:02:31,790 --> 00:02:35,290
+three types of blood smear طبعا blood smear أو
+
+30
+00:02:35,290 --> 00:02:39,290
+blood film نفس المعنى اللي هي مسحة الدم في عندي
+
+31
+00:02:39,290 --> 00:02:43,670
+تلت أنواع منهامسحة تجرع على ال cover glass أو اللي
+
+32
+00:02:43,670 --> 00:02:47,650
+هو ال .. ال .. ال cover slip اللي أنتوا كنتوا
+
+33
+00:02:47,650 --> 00:02:51,810
+تستخدموه لحتى تغطوا ال chamber ممكن أنا أفرد عليه
+
+34
+00:02:51,810 --> 00:02:56,730
+الدم فبسميه cover glass سمير ممكن أنا بسم .. أعمل
+
+35
+00:02:56,730 --> 00:03:02,490
+wedge سمير باستخدام شريحتين زجاجيات طبعا فبنسمي
+
+36
+00:03:02,490 --> 00:03:07,770
+هذا ال .. ال .. السمير wedge سمير طبعاأو ال sponge
+
+37
+00:03:07,770 --> 00:03:13,470
+smear باستخدام الأجهزة، تمام؟ يعني هو electronic
+
+38
+00:03:13,470 --> 00:03:18,730
+أو تقني، تقني، تمام؟ آلي، اذا في عندي تلت أنواع من
+
+39
+00:03:18,730 --> 00:03:21,670
+ال blood films أو ال blood smears، احنا اليوم راح
+
+40
+00:03:21,670 --> 00:03:25,850
+نتحدث و انت راح تتعلموا بالمختبر ال wedge smear،
+
+41
+00:03:25,850 --> 00:03:29,930
+تمام؟ اللي هو باستخدام two slides، باستخدام two
+
+42
+00:03:29,930 --> 00:03:34,840
+slidesطبعا في عندي أنواع أخرى من ال blood smears
+
+43
+00:03:34,840 --> 00:03:40,020
+أو ال blood film بيتم تحضيرهم for specific
+
+44
+00:03:40,020 --> 00:03:46,240
+purposes لأغراض خاصة مثلا buffy coat smearيعني أنه
+
+45
+00:03:46,240 --> 00:03:51,340
+أنا أفصل الدم باستخدام ال centrifuge و أخد عينة من
+
+46
+00:03:51,340 --> 00:03:54,680
+منطقة ال buffy code تمام علشان أنا أفرضها على ال
+
+47
+00:03:54,680 --> 00:04:02,640
+slide و أشوف ال white pieces أشكالها و عدادها و
+
+48
+00:04:02,640 --> 00:04:06,700
+هذه تجرى للناس اللي عندهم ال white pieces أقل من
+
+49
+00:04:06,700 --> 00:04:12,300
+ألف تمام يعني عندهم leukocytopenia يعني ليكو سايتس
+
+50
+00:04:12,300 --> 00:04:18,060
+قليلة تمامطيب في نوع آخر من ال blood films أو ال
+
+51
+00:04:18,060 --> 00:04:22,180
+blood smears بنسميه thick blood smears يعني أنه
+
+52
+00:04:22,180 --> 00:04:28,760
+أنا أحضر blood film سميك تمام؟ أحضر blood film
+
+53
+00:04:28,760 --> 00:04:33,580
+سميك وهذا بيستخدم لتشخيص ال blood parasites لتشخيص
+
+54
+00:04:33,580 --> 00:04:38,500
+الطفليات اللي بتعيش في الدم و بتسبب أمراض الدم زي
+
+55
+00:04:38,500 --> 00:04:44,230
+ال malaria مثلاطيب فإذا أنا بحضر لحتى مثلا أشخص
+
+56
+00:04:44,230 --> 00:04:47,570
+malaria لازم يكون في عندي thick blood film إيش
+
+57
+00:04:47,570 --> 00:04:50,330
+يعني thick blood film؟ يعني أنا بستخدم كمية كبيرة
+
+58
+00:04:50,330 --> 00:04:53,950
+من الدم و بفرضها على الشريحة على .. حتى تعطيني ثمك
+
+59
+00:04:53,950 --> 00:04:59,840
+.. تعطي ثماكة للفيلمطيب إيش نوع العينة اللي عندي؟
+
+60
+00:04:59,840 --> 00:05:03,020
+لازم تكون العينة edita anticoagulant ممكن أنا
+
+61
+00:05:03,020 --> 00:05:07,340
+أستخدم أنواع أخرى من ال .. ال anticoagulant ولكن
+
+62
+00:05:07,340 --> 00:05:11,100
+ال most common اللي أكثر شيوعا و أكثر استخداما هو
+
+63
+00:05:11,100 --> 00:05:15,640
+ال edita طبعا لازم تكون العينة خلال ساعتين أو
+
+64
+00:05:15,640 --> 00:05:20,920
+تلاتة من جامعهاانا اجري ال blood film او احضر ال
+
+65
+00:05:20,920 --> 00:05:26,640
+blood film ليش؟ لإنه اذا طولت العينة ممكن يحدث
+
+66
+00:05:26,640 --> 00:05:32,880
+change على ال RBCs morphology مثلا يعني انه تتغير
+
+67
+00:05:32,880 --> 00:05:37,360
+ال RBCs تبدأ خلايا تموت تتكسر ال white BCs ممكن
+
+68
+00:05:37,360 --> 00:05:41,580
+تتغير فبالتالي اذا انا لازم استخدم العينة تكون
+
+69
+00:05:41,580 --> 00:05:45,400
+fresh او خلال ساعتين لتلاتة من ساحبها انا اكون
+
+70
+00:05:45,400 --> 00:05:50,150
+حضرت ال blood filmطيب في عوامل أخرى أيضا تؤثر على
+
+71
+00:05:50,150 --> 00:05:54,090
+شكل ال RBC's ما يتشينج RBC's morphology ممكن
+
+72
+00:05:54,090 --> 00:05:57,150
+تخليها speculated او granulated يعني shrinkage
+
+73
+00:05:57,150 --> 00:06:00,950
+يصيرلها shrinkage تمام؟ ايش اللي ممكن يسببلي
+
+74
+00:06:00,950 --> 00:06:04,510
+الشرنكج؟ اكيد ال excessive amount of anticoagulant
+
+75
+00:06:04,510 --> 00:06:08,710
+يعني اذا انا مثلا ماعبيت دم في طيوب الاديتال عند
+
+76
+00:06:08,710 --> 00:06:12,610
+العلامة معناها الاديتال زيادة هتكون بالنسبة لل
+
+77
+00:06:12,610 --> 00:06:17,890
+bloodفي عندي excessive amount of anticoagulant،
+
+78
+00:06:17,890 --> 00:06:21,130
+إذا ال RBCs راح يصير .. يصيرلها shrinkage، راح ي
+
+79
+00:06:21,130 --> 00:06:25,790
+.. ال shrinkage يعني راح تبرز من الخلية إشي ما
+
+80
+00:06:25,790 --> 00:06:30,750
+يشبه ال .. ال .. الأشواك، تمام؟ إشي ما يشبه
+
+81
+00:06:30,750 --> 00:06:36,450
+الأشواكall the blood longstanding تمام all the
+
+82
+00:06:36,450 --> 00:06:41,470
+blood and longstanding يعني عينة قديمة أو طولت
+
+83
+00:06:41,470 --> 00:06:45,330
+أيضا بتتأثر على ال RBCs morphology و احنا كنا عم
+
+84
+00:06:45,330 --> 00:06:50,790
+نحكي ان ال RBCs او ال blood بشكل عام اذا طولت
+
+85
+00:06:50,790 --> 00:06:55,270
+العينة من ساعتين الى تلاتة او اكتر يعني تجاوزت
+
+86
+00:06:55,270 --> 00:07:01,180
+ساعتين لتلاتة فهذا معناه انهال RBCs رح يبدأ يتغير
+
+87
+00:07:01,180 --> 00:07:05,280
+شكلها ال warm environment .. ال warm environment
+
+88
+00:07:05,280 --> 00:07:10,040
+هادي بتقدي انه انا لما اجي .. يعني جو حار او بيئة
+
+89
+00:07:10,040 --> 00:07:14,880
+دافئة هتقدي الى انه و انا بفرد الدم على الشريحة
+
+90
+00:07:14,880 --> 00:07:21,710
+ممكن يتبخر سائلمن ال plasma تمام هذا راح يأدي إلى
+
+91
+00:07:21,710 --> 00:07:26,790
+جفاف ال blood بسرعة أو اللي هي زيادة تركيزه او
+
+92
+00:07:26,790 --> 00:07:33,370
+جفافه كتير فبالتالي هيأثر على ال .. ال ..ال RBCs
+
+93
+00:07:33,370 --> 00:07:36,470
+morphology ويمكن كنتوا تشوفوا أنتوا أثناء عد ال
+
+94
+00:07:36,470 --> 00:07:41,990
+RBCs على ال chamber لما كنتوا تطولوا بالعد كان يجف
+
+95
+00:07:41,990 --> 00:07:46,550
+ال saline و ال RBCs تلاحظوا أنها هربت إلى المنتصف
+
+96
+00:07:46,550 --> 00:07:50,510
+بحثا عن الرطوبة و تلاحظوا أنه على ال morphology
+
+97
+00:07:50,510 --> 00:07:55,890
+يعني على المربعات كانت ال RBCs تبدو زي كأنه
+
+98
+00:07:55,890 --> 00:07:59,710
+صاريلها shrinkage طبعا يعني صاريلها speculated أو
+
+99
+00:07:59,710 --> 00:08:04,170
+createdهلأ لحتى بس نعرف إيش يعني speculated أو
+
+100
+00:08:04,170 --> 00:08:08,810
+created بدي بس .. حتى أعرفكم إيش يعني ال .. ال ..
+
+101
+00:08:08,810 --> 00:08:16,350
+إنه ال RBCs يصيرلها speculated خليني أحكي
+
+102
+00:08:16,350 --> 00:08:20,330
+Speculated
+
+103
+00:08:20,330 --> 00:08:24,230
+RBCs
+
+104
+00:08:24,230 --> 00:08:31,510
+تمام؟ نشوف الصور إيش يعنيهيك high speculated يعني
+
+105
+00:08:31,510 --> 00:08:35,970
+إنها إلها زي .. زي أشواك، نطوقات بارزة منها، تمام؟
+
+106
+00:08:35,970 --> 00:08:39,590
+ها دي معناه إن ال RBCs صارلها shrinkage و يمكن
+
+107
+00:08:39,590 --> 00:08:42,850
+كنتوا تشوفوا هذا الشكل، كنتوا تشوفوا إن ال RBCs زي
+
+108
+00:08:42,850 --> 00:08:49,270
+إلها هيكأطراف معرجة أو يعني بارز منها بروزات و
+
+109
+00:08:49,270 --> 00:08:52,590
+أثناء ما كانت تجف العينة في عد ال RBCs نفس الاشي
+
+110
+00:08:52,590 --> 00:08:57,870
+أنا ممكن أشوفه في حالة ال blood film لو أنا كنت
+
+111
+00:08:57,870 --> 00:09:00,470
+بستخدم .. لو كان عندي excessive amount of
+
+112
+00:09:00,470 --> 00:09:04,930
+anticoagulant لو العينة قديمة أو حتى لو ال ..
+
+113
+00:09:04,930 --> 00:09:12,060
+البيئة حارة فأدت إلى جفاة في العينة بسرعة تمامطيب،
+
+114
+00:09:12,060 --> 00:09:15,900
+هلأ إيش التجربة اللي أنا بدي أجريها؟ أنا بدي أحط
+
+115
+00:09:15,900 --> 00:09:19,020
+قطرة من الدم، طبعا القطرة هذه لازم يكون حجمها
+
+116
+00:09:19,020 --> 00:09:23,100
+مناسب، ماتكونش كبيرة كتير أو صغيرة كتير على clean
+
+117
+00:09:23,100 --> 00:09:27,920
+glass slide، على glass slide، شريحة زجاجيةتمام؟ و
+
+118
+00:09:27,920 --> 00:09:32,560
+بدي أجيب شريحة أخرى و أفلت فيها هذا القطر عشان هيك
+
+119
+00:09:32,560 --> 00:09:38,100
+أنا بستخدم شريحتين زجاجيتين تمام؟ و طبعا الشريحة
+
+120
+00:09:38,100 --> 00:09:41,460
+اللي أنا بفلت فيها بكون مثبتاها بزاوية من تلاتين
+
+121
+00:09:41,460 --> 00:09:45,870
+لاربعينو بتحكم طبعا هذه الزاوية هي اللي بتتحكملي
+
+122
+00:09:45,870 --> 00:09:49,210
+بال thickness of the smear و هلأ راح نعرف إيش يعني
+
+123
+00:09:49,210 --> 00:09:55,350
+و طبعا بعد ما أنا بفرد الدم على الشريحة بدي أخليه
+
+124
+00:09:55,350 --> 00:10:02,720
+جف خلال الهواء بعدين أنا أحضره للصباغةهذا كل ما
+
+125
+00:10:02,720 --> 00:10:06,180
+يتعلق بالتجربة طبعا بيحكيلي هنا أنه مش لازم أنا
+
+126
+00:10:06,180 --> 00:10:12,040
+أنفخ على الشريحة لحتى تجف، بدي أتركها تجف لحالها
+
+127
+00:10:12,040 --> 00:10:16,700
+لإنه اللي نفخ مع البخار اللي بيطلع ممكن يقدي إلى
+
+128
+00:10:16,700 --> 00:10:20,160
+RBCs artifact يعني أن ال RBCs تتشوه أو يصير في
+
+129
+00:10:20,160 --> 00:10:25,380
+عليها أشياء داخلة مش من .. مش من الخلية ذات نفسها
+
+130
+00:10:26,360 --> 00:10:32,080
+طيب إذا أنا بدي أحكينا place drop of blood بدي أحط
+
+131
+00:10:32,080 --> 00:10:36,500
+قطرة .. قطرة من الدم على الشريحة و باستخدام شريحة
+
+132
+00:10:36,500 --> 00:10:40,880
+أخرى بدي أفرد هذه القطرة طبعا بهذا الشكل بأحطها
+
+133
+00:10:40,880 --> 00:10:45,900
+باتجاه معاكس بخلي قطرة الدم تفرد على طرف الشريحة و
+
+134
+00:10:45,900 --> 00:10:49,980
+ب .. بحركها بهذا الاتجاه بحيث انها .. انه انا كون
+
+135
+00:10:49,980 --> 00:10:54,280
+فردت العينة تمام؟ و هيك بيكون شكل ال .. ال .. ال
+
+136
+00:10:54,280 --> 00:10:59,240
+..مسحة الدم، الدم تم مسحه على الشريحة و هيك بيكون
+
+137
+00:10:59,240 --> 00:11:03,140
+شكله في النهاية بتركه to .. to air dry علشان يجف
+
+138
+00:11:03,140 --> 00:11:09,240
+بالهواءطيب characteristics of good smear إيش هي
+
+139
+00:11:09,240 --> 00:11:14,100
+خواص أو خصائص ال good smear لحتى أحكي أنا هذا
+
+140
+00:11:14,100 --> 00:11:20,460
+الفيلم أو هذه المسحة للدم جيدة أو مواصفاتها جيدة
+
+141
+00:11:20,460 --> 00:11:25,680
+إيش لازم .. إيش الشروط اللي لازم تتحقق فيها؟أول
+
+142
+00:11:25,680 --> 00:11:31,120
+حاجة thick at one end, thinning out to a smooth
+
+143
+00:11:31,120 --> 00:11:34,980
+round feather edge هلأ إيش يعني؟ لازم تكون thick
+
+144
+00:11:34,980 --> 00:11:39,580
+في منطقة و thin في منطقة أخرى، thick في منطقة ال
+
+145
+00:11:39,580 --> 00:11:43,540
+drop عند منطقة ال drop هيكون في أعلى تركيز للدم
+
+146
+00:11:43,540 --> 00:11:49,200
+يعني هتكون thick سميكهيبدأ يخف تركيزه قدام مع أنه
+
+147
+00:11:49,200 --> 00:11:53,360
+كل ما بمسح لقدام شوية معناه أنه هيكون في عندي أقل
+
+148
+00:11:53,360 --> 00:11:58,680
+تركيز فبالتالي هتكون هي سن يعني و لازم تكون
+
+149
+00:11:58,680 --> 00:12:03,060
+أطرافها دائرية و منتظمة ما .. مايتكون مشرشرة
+
+150
+00:12:03,060 --> 00:12:06,700
+أطرافها بهذا الشكل زي ما أنتوا شايفين هي أطرافها
+
+151
+00:12:06,700 --> 00:12:13,870
+دائرية و منتظمةطيب occupy two thirds of the total
+
+152
+00:12:13,870 --> 00:12:18,610
+slide area تحتل تقريبا تلتان الشريحة و أيضا
+
+153
+00:12:19,700 --> 00:12:22,720
+shouldn't touch any edge of the slide مش لازم
+
+154
+00:12:22,720 --> 00:12:28,760
+تلامس أي طرف أو أي حافة من حواف الشريحة تمام؟
+
+155
+00:12:28,760 --> 00:12:34,400
+وكمان should be margin free مايكونش فيها فراغات مع
+
+156
+00:12:34,400 --> 00:12:40,940
+ده منطقة القطرة هاي هنا أنا حطيت القطرة و فردت
+
+157
+00:12:40,940 --> 00:12:46,020
+الشريحة مكان القطرة هيصير في عندي زي الفراغطبعا
+
+158
+00:12:46,020 --> 00:12:50,260
+هذا مسموح فيه لكن لازم المسحة تكون منتظمة ومستمرة
+
+159
+00:12:50,260 --> 00:12:54,800
+ان الدم مفروض بشكل مستمر ومايكون فيه فراغات should
+
+160
+00:12:54,800 --> 00:13:00,460
+be margin free طبعا و as soon as the drop of blood
+
+161
+00:13:00,460 --> 00:13:04,400
+is placed on the glass slide the smear should be
+
+162
+00:13:04,400 --> 00:13:08,060
+made without delay مجرد ما احط قطرة الدم على
+
+163
+00:13:08,060 --> 00:13:12,700
+الشريحة لازم انا اعمل الفردة او ال smearالمسحة
+
+164
+00:13:12,700 --> 00:13:18,600
+بشكل سريع بدون تأخير لإنه أي تأخير راح يقدي إلى
+
+165
+00:13:18,600 --> 00:13:22,220
+أول حاجة abnormal distribution of the white blood
+
+166
+00:13:22,220 --> 00:13:29,380
+cell تمام تاني إشي ممكن حواف القطرة يجفوا وبالتالي
+
+167
+00:13:29,380 --> 00:13:33,280
+يجرحوا الفردة يعني الفردة تصير فيها فراغة تصير
+
+168
+00:13:33,280 --> 00:13:38,490
+فيها تجريحات ما تكونش هيك مستمرة وملساقطبعا
+
+169
+00:13:38,490 --> 00:13:42,990
+فبالتالي بسبب الدم الجاف فبالتالي انا لازم انا
+
+170
+00:13:42,990 --> 00:13:48,090
+اعمل ال .. ال .. الفرد ل .. ل .. ل blood film بشكل
+
+171
+00:13:48,090 --> 00:13:54,070
+سريع طبعا؟ طيب و حكينا ايضا ان هذا بيأثر ..
+
+172
+00:13:54,070 --> 00:13:56,770
+التأخير في الفرد راح يأثر على distribution of
+
+173
+00:13:56,770 --> 00:14:00,930
+white blood cellيعني مثلا ال large white cells
+
+174
+00:14:00,930 --> 00:14:04,530
+accumulating at the thin edges of the smear ممكن
+
+175
+00:14:04,530 --> 00:14:07,950
+يخلي انه ال large white cells يعني الخلايا الكبيرة
+
+176
+00:14:07,950 --> 00:14:13,110
+زي ال .. ال monocyte و ال neutrophil هدولة يروحوا
+
+177
+00:14:13,110 --> 00:14:16,990
+على الأطراف اللي .. اللي هي very thin هنافي الآخر
+
+178
+00:14:16,990 --> 00:14:20,850
+و لما اجى اقعد مثلا انا بعيد في المنطقة اللى بتكون
+
+179
+00:14:20,850 --> 00:14:24,390
+sin فلما اجى اقعد والله ممكن انا احكم ان الشخص هذا
+
+180
+00:14:24,390 --> 00:14:28,890
+مثلا عنده neutrophilia او عنده monocytosis يعني
+
+181
+00:14:28,890 --> 00:14:31,610
+عدد كبير من ال neutrophils او عدد كبير من ال
+
+182
+00:14:31,610 --> 00:14:36,930
+monocytes هذا ممكن يكون خاطر انه بس اتغيرت توزيع
+
+183
+00:14:36,930 --> 00:14:42,970
+الخلايا بسبب اني انا اتأخرت في فرد الشريحة تمام؟
+
+184
+00:14:43,740 --> 00:14:49,000
+طيب the thickness of the spread كيف أنا أتحكم بصمك
+
+185
+00:14:49,000 --> 00:14:55,900
+الفرد أو صمك ال .. ال blood smear فعليا أنا بتحكم
+
+186
+00:14:55,900 --> 00:14:59,700
+فيه من خلال الزاوية حكينا أنا ب .. بستعمل زاوية من
+
+187
+00:14:59,700 --> 00:15:07,660
+تلاتين لاربعين تمام؟ لكن لو أنا شخص أجاني و عنده
+
+188
+00:15:07,660 --> 00:15:12,300
+ال hematocrite عاليتمام؟ ال hemotocrit عالي يعني
+
+189
+00:15:12,300 --> 00:15:19,280
+عنده RBC's يعني كتيرة تمام؟ فبالتالي هذا الشخص أنا
+
+190
+00:15:19,280 --> 00:15:25,360
+لازم أعمله فيلم طويل تمام؟ أفرد الدم على أكبر
+
+191
+00:15:25,360 --> 00:15:29,780
+مساحة ممكنة لازم ال .. ال .. الدم يكون .. ال .. ال
+
+192
+00:15:29,780 --> 00:15:34,490
+.. السمير يكون .. الفيلم يكون طويل و يكون سنفهذا
+
+193
+00:15:34,490 --> 00:15:38,770
+إيش بدي أعمل مع هذه الحالة؟ بدي أقلل الزاوية،
+
+194
+00:15:38,770 --> 00:15:43,450
+تمام؟ بقلل الزاوية فالدم بيفرض معايا كمية أكبر و
+
+195
+00:15:43,450 --> 00:15:47,650
+هيك بيطلع معايا thin طيب لو كان في عندي لوح
+
+196
+00:15:47,650 --> 00:15:54,520
+ماتكرتني يعني الخلايا الدم تركيزها قليلتمام؟ هذا
+
+197
+00:15:54,520 --> 00:15:58,560
+معناه انه انا لازم يكون في عندي ثمك لل .. لل blood
+
+198
+00:15:58,560 --> 00:16:03,140
+film علشان الخلايا ماتكونش هيك بعيدة عن بعض و
+
+199
+00:16:03,140 --> 00:16:08,380
+متفرقة فلازم انا اعلي الزاوية تمام؟ اكبر الزاوية
+
+200
+00:16:08,380 --> 00:16:12,620
+بحيث انه هاي الزاوية اللي انا كبرتها تفردلي الدم
+
+201
+00:16:12,620 --> 00:16:18,660
+على مساحة اقل تمام؟ فبالتالي يكون هو thickماشي،
+
+202
+00:16:18,660 --> 00:16:24,000
+فإذا الزاوية بتتحكملي بطول وسمك ال .. ال .. الفيلم
+
+203
+00:16:24,000 --> 00:16:29,240
+كل ما قلت الزاوية ال .. الفيلم هيكون طويل و سن و
+
+204
+00:16:29,240 --> 00:16:32,140
+هذا بيظبط مع الناس اللي ال hemotocrit عندهم عالي
+
+205
+00:16:32,140 --> 00:16:38,300
+كل ما زادت الزاوية هيكون ال .. ال .. الفيلم قصير و
+
+206
+00:16:38,300 --> 00:16:40,840
+سميك فبالتالي هذا بيظبط مع الناس اللي ال
+
+207
+00:16:40,840 --> 00:16:46,330
+hemotocrit عندهم قليلطيب common causes of boring
+
+208
+00:16:46,330 --> 00:16:51,390
+blood film هلأ إيش ممكن يسببلي أو يعني إيش ممكن
+
+209
+00:16:51,390 --> 00:16:55,890
+يحصل لحتى أحكي عن ال blood film هذا بور سيء أو غير
+
+210
+00:16:55,890 --> 00:17:03,510
+مقبول لقراية أشكال و أعداد و أنواع الخلايا؟هلأ أول
+
+211
+00:17:03,510 --> 00:17:06,990
+حاجة ل drop of blood is too large or too small لو
+
+212
+00:17:06,990 --> 00:17:11,370
+كانت قطرة الدم كتير كبيرة معناها الفيلم هيكون كتير
+
+213
+00:17:11,370 --> 00:17:15,650
+سميك فبالتالي ممكن يعيق عملية التصنيف أنواع
+
+214
+00:17:15,650 --> 00:17:19,750
+الخلايا و دراسة ال morphology تبعها، دراسة شكلهاو
+
+215
+00:17:19,750 --> 00:17:24,010
+اذا كان نفس الاشي ال .. ال .. القطر سميكة كتير
+
+216
+00:17:24,010 --> 00:17:28,790
+ممكن تعطيني فيلم صغير و الفيلم هذا او .. او يكون
+
+217
+00:17:28,790 --> 00:17:34,850
+thin فبالتالي ماتعبرش عن وضع الخلايا، تمام؟
+
+218
+00:17:34,850 --> 00:17:38,890
+فبالتالي لازم يكون الحجم مناسب و ال spreader slide
+
+219
+00:17:38,890 --> 00:17:42,270
+pushed across the slide in jerky manner لو احنا
+
+220
+00:17:44,170 --> 00:17:50,030
+فردنا الدم بشكل متقطع تمام فهذا برضه كمان غلط هذا
+
+221
+00:17:50,030 --> 00:17:54,970
+هيطلع معايا ال .. ال .. الدم هيك بشكل .. بهك ..
+
+222
+00:17:54,970 --> 00:17:58,490
+بهذا الشكل لو أنا مثلا كنت بفرد في الدم و ب .. ب
+
+223
+00:17:58,490 --> 00:18:03,770
+.. ب .. يعني بفرد و ب .. بقففمع الفرد المتقطع هذا
+
+224
+00:18:03,770 --> 00:18:07,510
+راح تظهر عندى اكتر من منطقة وما بينهم فراغات و هذا
+
+225
+00:18:07,510 --> 00:18:11,050
+غير مقبول، هذا احنا حكينا انه من شروط ال blood
+
+226
+00:18:11,050 --> 00:18:14,730
+film انه يكون margin free مافيش فيه فراغات، فإذا
+
+227
+00:18:14,730 --> 00:18:20,170
+هذا غلططيب بعدين لازم كمان failure to keep the
+
+228
+00:18:20,170 --> 00:18:23,210
+entire edge of the spreader slide against the
+
+229
+00:18:23,210 --> 00:18:26,990
+slide while making the smear هاي جملة معناها انه
+
+230
+00:18:26,990 --> 00:18:34,210
+انا يعني هو بصراحة و انا بفرض في ال .. في ال blood
+
+231
+00:18:34,210 --> 00:18:39,190
+film لازم انا اكمل فرد لآخر الشريحة يعني ما اتوقف
+
+232
+00:18:39,190 --> 00:18:44,780
+فجأةلحد ما يخلص معايا الدم لحاله بي .. بيعطي الدم
+
+233
+00:18:44,780 --> 00:18:48,340
+.. لو أول ما يخلص الدم لحاله بيعطي الشكل الدائري،
+
+234
+00:18:48,340 --> 00:18:52,680
+الطرف الدائري هذا، تمام؟ طيب لو أنا .. يعني ما
+
+235
+00:18:52,680 --> 00:18:56,360
+استمرت بالحركة لآخر ال slide، توقفت فجأة، إيش رح
+
+236
+00:18:56,360 --> 00:19:01,040
+يصير في عندى؟ رح يصير بهذا الشكل، إنه في عندي ال
+
+237
+00:19:01,040 --> 00:19:04,860
+.. ال .. ال blood film نهايته بدل ما هي دائرية،
+
+238
+00:19:04,860 --> 00:19:09,520
+نهايته إلها خط، يعني مثلا نتوقع .. نتخيل هذا
+
+239
+00:19:09,520 --> 00:19:14,660
+الفيلمهيك و أنا توقفت فجأة نهايته بدل ما هي دائرية
+
+240
+00:19:14,660 --> 00:19:18,960
+هيك خط، تمام؟ فهد معناها انه انا .. ان انا ما
+
+241
+00:19:18,960 --> 00:19:23,020
+استمرت بالحركة على ال entire slide يعني ماكملت
+
+242
+00:19:23,020 --> 00:19:29,070
+الحركة للاخر فهد غلطfailure to keep the spreader
+
+243
+00:19:29,070 --> 00:19:34,610
+slide at 30 degree angle طبعا اذا انا ما .. يعني
+
+244
+00:19:34,610 --> 00:19:38,110
+ماخلت الزاوية تلاتين او من تلاتين لاربعين فهذا
+
+245
+00:19:38,110 --> 00:19:41,650
+معناها انه انا اذا كبرت الزاوية هيكون عندى short و
+
+246
+00:19:41,650 --> 00:19:48,570
+thick او اذا زغرتها فهيكون عندى thin و long و هذا
+
+247
+00:19:48,570 --> 00:19:52,680
+غلططمام؟ انا فعليا بس بكبر الزاوية في حالة ال
+
+248
+00:19:52,680 --> 00:19:56,120
+decreased hematocrit و بظغر الزاوية في حالة ال
+
+249
+00:19:56,120 --> 00:19:59,780
+increased hematocrit شخص normal او ماعندوش المشكلة
+
+250
+00:19:59,780 --> 00:20:03,880
+هاي فبالتالي لازم الزاوية تكون من 30 ل 40 failure
+
+251
+00:20:03,880 --> 00:20:06,900
+to push the spreader slide completely across the
+
+252
+00:20:06,900 --> 00:20:11,030
+slide حكينا اذا انا ما .. اذا ماحطيتالشريحة اللي
+
+253
+00:20:11,030 --> 00:20:17,290
+أنا بفرد فيها ماكملت حركتها لآخر الشريحة اللي أنا
+
+254
+00:20:17,290 --> 00:20:22,750
+بفرد عليها فبالتالي هذا برضه كمان راح يعطيني سمير
+
+255
+00:20:22,750 --> 00:20:27,170
+نهايته مش دائرية irregular spread with ridges and
+
+256
+00:20:27,170 --> 00:20:33,030
+long tail إذا كان في عنديال .. الفرد مش منتظم و
+
+257
+00:20:33,030 --> 00:20:38,770
+إيه له حواف أو إيه له زي أطراف يعني مغير منتظمة
+
+258
+00:20:38,770 --> 00:20:43,750
+هذا معناه إنه الشريحة اللي أنا بفرض فيها هي إما
+
+259
+00:20:43,750 --> 00:20:50,150
+dirty عليها غبرة أو ممكن تكون مكسرة يعني في ..
+
+260
+00:20:50,150 --> 00:20:56,300
+فيها خدوش أو كسور فهذا بقى أيضا كمان ممكن يأثرHole
+
+261
+00:20:56,300 --> 00:21:01,560
+in films إذا حسيت إنه ال blood film فيه خفورات
+
+262
+00:21:01,560 --> 00:21:07,180
+فهذا معناها إن الشريحة عليها fat أو عليها دهون
+
+263
+00:21:07,180 --> 00:21:12,080
+تمام عليها زيت مثلا فهذه لازم أنا أنظفها بالكحول و
+
+264
+00:21:12,080 --> 00:21:16,820
+أستخدمها بعدين أو أنا أستخدم يعني نوعية أفضل في
+
+265
+00:21:16,820 --> 00:21:20,580
+عندي بعض الشرائح هي already أصلا تيجي محفوظة بشمع
+
+266
+00:21:21,050 --> 00:21:24,790
+فيعني عشان ما تجرح بعض هالبعض فبيكون في عليها طبقة
+
+267
+00:21:24,790 --> 00:21:30,530
+رفيعة من الشامع فالمفروض إنه إذا كانت الشريحة أو
+
+268
+00:21:30,530 --> 00:21:34,990
+الشرائح اللي موجودة عندي من هذا النوع فالمفروض إن
+
+269
+00:21:34,990 --> 00:21:38,630
+أنا أنجعها في الكحول ليلة قبل ما أستخدمها يعني
+
+270
+00:21:38,630 --> 00:21:45,210
+overnightتكون محطوطة في .. في jar في كحول قبل
+
+271
+00:21:45,210 --> 00:21:48,910
+الاستخدام علشان الكحول يعمل على إذابة الشمع اللي
+
+272
+00:21:48,910 --> 00:21:52,390
+موجود عليها Cellular degenerative changes إذا كان
+
+273
+00:21:52,390 --> 00:22:00,990
+في عنديتأخير في عملية ال fixation في تثبيت الفيلم
+
+274
+00:22:00,990 --> 00:22:04,110
+على الشريحة أو حتى ال fixative اللي أنا بستخدمه
+
+275
+00:22:04,110 --> 00:22:07,790
+اللي هو ال methanol إذا تم تلويثه بماء أو يعني
+
+276
+00:22:07,790 --> 00:22:11,190
+إيجي عليه ماء أو مطص رطوبة فهذا راح يأثر على
+
+277
+00:22:11,190 --> 00:22:14,210
+الخلايا ممكن يقدي إلى تحلولها تمام؟ يعمللي
+
+278
+00:22:14,210 --> 00:22:18,070
+cellular degenerative changes إذا هذه كلها أسباب
+
+279
+00:22:18,070 --> 00:22:22,760
+ممكن إنها تأثر على جودة ال blood filmهي هذه الو في
+
+280
+00:22:22,760 --> 00:22:26,000
+either edge أو irregular edges يعني أطراف مشارشرة
+
+281
+00:22:26,000 --> 00:22:30,160
+هذا نتيجة إنه إما الشريحة مش نظيفة عليها غبرة أو
+
+282
+00:22:30,160 --> 00:22:33,520
+إن الشريحة اللي أنا بفرض فيها أطرافها مكسرة أو في
+
+283
+00:22:33,520 --> 00:22:37,180
+عليها شقوق هذا ال jerky manner اللي هو الأسلوب
+
+284
+00:22:37,180 --> 00:22:41,580
+المتقطع هذا إنه يعني يُقال إنه كويس بس المفروض إنه
+
+285
+00:22:41,580 --> 00:22:45,300
+يكون أطول شوية هذا too short قليل جدا نتيجة إنه
+
+286
+00:22:45,300 --> 00:22:52,250
+أنا مثلاكبرت الزاوية هذا ارفيع كتير مشكلته أنه
+
+287
+00:22:52,250 --> 00:22:56,150
+يعني لازم يكون هو أعرض من هيك شوية هذه فيها holes
+
+288
+00:22:56,150 --> 00:23:03,470
+نتيجة أن الشريحة عليها شمع أو عليها زيت تمام oil
+
+289
+00:23:03,470 --> 00:23:09,470
+هذه أنه معناها .. هذه الشكل يعني أنه إيدي مالت هيك
+
+290
+00:23:09,470 --> 00:23:13,640
+انتشوحتباتجاه معين و انا بفرد فلأ لازم انا اضل على
+
+291
+00:23:13,640 --> 00:23:18,820
+ال entire هيك ما ا .. ما اميل و اضل باتجاه يعني
+
+292
+00:23:18,820 --> 00:23:22,980
+مستقيم و هذا هيو بيحكيلك failure to keep the
+
+293
+00:23:22,980 --> 00:23:25,720
+entire edge of the spreader slide against the
+
+294
+00:23:25,720 --> 00:23:31,800
+slide while making the smile يعني لازم لحظة
+
+295
+00:23:33,580 --> 00:23:39,080
+لازم حركة ال .. ال .. الشريحة الفاردة اللي بفرض
+
+296
+00:23:39,080 --> 00:23:47,080
+فيها تكون يعني على نفس اتجاه الشريحة، تمام؟ الحركة
+
+297
+00:23:47,080 --> 00:23:49,860
+على نفس الاتجاه، ولكن لما أنا بتبتها، بتبتها
+
+298
+00:23:49,860 --> 00:23:53,140
+بالاتجاه المعاكس، وهذا الكلام كله هورجيكم إياه في
+
+299
+00:23:53,140 --> 00:23:56,460
+العمل، إيش معناه؟ تمام؟ هنا في عندي air drop عشان
+
+300
+00:23:56,460 --> 00:24:01,980
+هيك طلعت في عندي منطقة فاضية في نص القطرة أو في نص
+
+301
+00:24:01,980 --> 00:24:07,970
+الفيلمتمام biological causes of a poor smear يعني
+
+302
+00:24:07,970 --> 00:24:14,430
+أسباب يعني بتأدي إلى أنه السمير يكون سيئ أو رديق
+
+303
+00:24:14,430 --> 00:24:20,110
+ولكن المشكلة في العينة و ليس في تحضير الفيلم أول
+
+304
+00:24:20,110 --> 00:24:27,000
+إشي called agglutininمشكلة إن ال RBCs إذا ما كانت
+
+305
+00:24:27,000 --> 00:24:30,620
+موجودة على درجة حرارة أقل من 37 أو على درجة حرارة
+
+306
+00:24:30,620 --> 00:24:36,620
+الغرفة مثلا ممكن إنه الدم يتجمع أو يتجلط حتى لو
+
+307
+00:24:36,620 --> 00:24:39,300
+أنا حاطة في anticoagulant ممكن يتجلط نتيجة وجود
+
+308
+00:24:39,300 --> 00:24:42,340
+antibody بيجمع ال RBCs مع بعضها البعض و بيقدي إلى
+
+309
+00:24:42,340 --> 00:24:47,640
+aggregation فالحل إنه طبعا هذا ال antibody بيشتغل
+
+310
+00:24:47,640 --> 00:24:51,340
+على درجة حرارة منخفضة يعني درجة حرارة الغرفة فأقل
+
+311
+00:24:51,340 --> 00:24:56,530
+من 25 و تحتطيب فالحل انه انا ادفع العينة احطها على
+
+312
+00:24:56,530 --> 00:25:00,230
+37 علشان هذا ال antibody فك عن الخلايا و يبطل
+
+313
+00:25:00,230 --> 00:25:05,610
+مجمعها و يبطل في عندي agglutination تمام؟ فالفكرة
+
+314
+00:25:05,610 --> 00:25:10,770
+انه انا لو لاحظت انه في شخص عنده هذه المشكلة عنده
+
+315
+00:25:10,770 --> 00:25:14,450
+antibody ممكن يجمع ال RBC يعني عنده حالة called
+
+316
+00:25:14,450 --> 00:25:18,330
+agglutininتمام؟ اللازم إن أنا أعمل incubation لل
+
+317
+00:25:18,330 --> 00:25:22,690
+RBCs على 37 درجة أحطها مثلا في ال water bath أو في
+
+318
+00:25:22,690 --> 00:25:26,190
+ال incubator بعدين أدفع العينة بعدين أحضر منها
+
+319
+00:25:26,190 --> 00:25:33,280
+لسمير لايبينيا إذا الشخص عنده دهونعالية تمام
+
+320
+00:25:33,280 --> 00:25:36,480
+lipemia يعني نسبة الدهون عالية او مستوى الدهون
+
+321
+00:25:36,480 --> 00:25:40,880
+عالي تمام فهذا معناه انه راح يظهر holes حفورات في
+
+322
+00:25:40,880 --> 00:25:44,260
+ال blood film هذه الصراحة المشكلة انا مابقدر يعني
+
+323
+00:25:44,260 --> 00:25:50,570
+احلها لو كانت المشكلة على الفيلمإذا كان ال .. يعني
+
+324
+00:25:50,570 --> 00:25:53,410
+إذا كانت مشكلة الدهون موجودة على الشريحة فأنا ممكن
+
+325
+00:25:53,410 --> 00:25:55,850
+أنظف الشريحة بس إذا كانت الدهون موجودة من العين
+
+326
+00:25:55,850 --> 00:26:01,290
+ذات نفسها أنا ما .. يعني لأ أستطيع إن أنا أصحح هذا
+
+327
+00:26:01,290 --> 00:26:07,250
+الخطأ أو هذه المشكلةRouleau Formation إذا ال RBCs
+
+328
+00:26:07,250 --> 00:26:10,810
+و إنها هي بتعمل stack resembling coins هذا طبعا
+
+329
+00:26:10,810 --> 00:26:13,570
+تعريف ال Rouleau Formation و أظن إنه مار معانا في
+
+330
+00:26:13,570 --> 00:26:18,950
+معمال ال ESR طبعا هذا نتيجة إنه في عندي بروتينات
+
+331
+00:26:18,950 --> 00:26:21,970
+عالية بتجم بت .. بتقلل المسافة ما بين ال RBCs
+
+332
+00:26:21,970 --> 00:26:25,310
+بتقدي إلى تجمعها و ال .. و البروتينات هذه بتكون
+
+333
+00:26:25,310 --> 00:26:28,330
+مرتبطة بحالات مرضية معينة زي ال infection و ال
+
+334
+00:26:28,330 --> 00:26:31,380
+inflammation و ال tissue injuryو الصراحة انا ..
+
+335
+00:26:31,380 --> 00:26:34,820
+يعني اذا الشخص عنده مشكلة مرضية فانا مابقدر أحلها
+
+336
+00:26:34,820 --> 00:26:40,300
+لحتى أمنع انه يتكون ال role and أثناء فرد العينة
+
+337
+00:26:40,300 --> 00:26:47,720
+تمام؟ فهذا طبعا ال .. الاشي مابقدر أحله تقرأ
+
+338
+00:26:47,720 --> 00:26:52,500
+الشريحة و بنعملها examination و هي موجودة لهذه
+
+339
+00:26:52,500 --> 00:26:57,380
+المشكلة عادي يعني بنحاول نعمل .. نعملها skip طيب
+
+340
+00:26:59,340 --> 00:27:03,800
+although this is the easiest and most popular
+
+341
+00:27:03,800 --> 00:27:07,020
+method for producing a blood smear it doesn't
+
+342
+00:27:07,020 --> 00:27:11,680
+produce quality smear as a result of هلأ صح ال ..
+
+343
+00:27:11,680 --> 00:27:15,320
+الوجسمير طريقة الوجسمير اللي هي السمير اللي بيتحدر
+
+344
+00:27:15,320 --> 00:27:20,280
+ب two slides هي أفضل طريقة .. يعني أسهل طريقة هي
+
+345
+00:27:20,280 --> 00:27:25,010
+ال easiest الأسهل و ال most popular الأكثرشيوعا
+
+346
+00:27:25,010 --> 00:27:29,870
+ولكن هي ما بتعطيني يعني سمير جودته عالية جدا
+
+347
+00:27:29,870 --> 00:27:34,270
+مقارنة مثلا بال automated بال .. بال spun تمام؟
+
+348
+00:27:34,270 --> 00:27:41,690
+ليش؟ لأنه أول حاجة ال white PCs أحيانا ما بتتوزعش
+
+349
+00:27:41,690 --> 00:27:44,350
+بشكل منتظم وممكن ال .. و ال RPCs بيصيرلها
+
+350
+00:27:44,350 --> 00:27:49,690
+distortion بتتشوّه شكلها على الحوافأيضا إذا كانت
+
+351
+00:27:49,690 --> 00:27:56,990
+أطراف السمير مشرشرة زي هيك فإنه في عندي .. إنه ال
+
+352
+00:27:56,990 --> 00:27:59,410
+.. ال smaller white pieces زي ال lymphocyte ال
+
+353
+00:27:59,410 --> 00:28:02,850
+smaller white pieces زي ال lymphocyte بتكون موجودة
+
+354
+00:28:02,850 --> 00:28:07,170
+في .. في منتصف هذه الأطراف المشرشرة و ال large
+
+355
+00:28:07,170 --> 00:28:12,610
+lymphocyte اللي هي زي ال monocyte بتكون موجودة على
+
+356
+00:28:12,610 --> 00:28:16,430
+الحواف تمام؟ بتكون موجودة على الحواف يعني لو نيجي
+
+357
+00:28:16,430 --> 00:28:20,280
+هناهنا مثلا على هذه الحافة في المنتصف فيه
+
+358
+00:28:20,280 --> 00:28:24,760
+lymphocyte و على الحواف على الأطراف بيكون موجود ال
+
+359
+00:28:24,760 --> 00:28:28,080
+.. ال monocyte اللي هي ال large lymphocyte .. ال
+
+360
+00:28:28,080 --> 00:28:32,580
+large white piecesSpun Smear reproduce the most
+
+361
+00:28:32,580 --> 00:28:35,460
+uniform distribution of a blood cell يعني ال Spun
+
+362
+00:28:35,460 --> 00:28:39,920
+Smear أو ال automated Smear بيعطيني يعني توزيع
+
+363
+00:28:39,920 --> 00:28:46,620
+أفضل ل .. ل .. ل blood cells طيب حضرت أنا ال ..
+
+364
+00:28:46,620 --> 00:28:52,880
+الشريحة فردت ال blood عملت فيلم و خليته air dry
+
+365
+00:28:52,880 --> 00:28:57,990
+يجف بالهواء هلأ دور اني انا اعملfixation and
+
+366
+00:28:57,990 --> 00:29:02,150
+staining تمام؟ fixation and staining هلأ بنعمل
+
+367
+00:29:02,150 --> 00:29:05,690
+fixation تثبيت لهذه العينة أو لهذا الدم على
+
+368
+00:29:05,690 --> 00:29:10,750
+الشريحة بدي استخدم fixative تمام؟ و هذا ال
+
+369
+00:29:10,750 --> 00:29:14,190
+fixative إيش دوره؟ preserve the morphology of cell
+
+370
+00:29:14,190 --> 00:29:19,650
+بيحافظ على شكل الخلايا تمام؟علشان أتضالها محتفظة
+
+371
+00:29:19,650 --> 00:29:23,290
+بشكلها و لما أجي أنا أدرس ال morphology تبعها يعني
+
+372
+00:29:23,290 --> 00:29:26,630
+أكون شكلها معبر زي كأنها موجودة في ال circulation
+
+373
+00:29:26,630 --> 00:29:33,770
+فبالتالي يعني أشخص صح طيب إذا ال .. ال .. ال films
+
+374
+00:29:33,770 --> 00:29:38,490
+must be fixed لازم .. لازم أثبتها و على طول أول ما
+
+375
+00:29:38,490 --> 00:29:42,210
+اتجفباش بدي أثبتها باستخدام fixative ال fixative
+
+376
+00:29:42,210 --> 00:29:45,550
+اللي أنا بستخدمه ال methyl alcohol اللي هو ال
+
+377
+00:29:45,550 --> 00:29:49,690
+methanol أو ممكن أستخدم ال ethyl alcohol اللي هو
+
+378
+00:29:49,690 --> 00:29:53,290
+ال ethanol طبعا لازم يكون absolute ال methanol
+
+379
+00:29:53,290 --> 00:29:56,670
+طبعا اللي أنا بستخدمه لازم يكون pure و ال ethanol
+
+380
+00:29:56,670 --> 00:30:00,610
+طبعا احنا انتوا ماري عليكم 70% ethanol يعني انا
+
+381
+00:30:00,610 --> 00:30:04,850
+بحضر مثلا باخد 70% من ال ethanol و بحط عليه 30
+
+382
+00:30:04,850 --> 00:30:12,990
+water تمام؟لأ لكن كلمة absolute هذا معناها انه
+
+383
+00:30:12,990 --> 00:30:20,270
+الethyl يعني تقريبا 99.9% pure مافيش عليه اي قطرة
+
+384
+00:30:20,270 --> 00:30:24,250
+ماء تمام؟ و ال methanol لازم ايضا يكون pure مش
+
+385
+00:30:24,250 --> 00:30:29,010
+مخلوط بماء زي ما بنشتريه من الشركة مايكونش عليه
+
+386
+00:30:29,010 --> 00:30:34,730
+ماء او معموله dilution بالماء نهائيا تمام؟ ليش؟
+
+387
+00:30:34,730 --> 00:30:39,920
+لإن الماءبيقدّي إلى تغيرات، degenerative changes،
+
+388
+00:30:39,920 --> 00:30:44,580
+بيقدّي إلى تحلل الخلايا أو تكسّرها أو ممكن يعملي
+
+389
+00:30:44,580 --> 00:30:51,520
+artifact، يعيق عملية الرؤية، فبالتالي لازم يكون ال
+
+390
+00:30:51,520 --> 00:30:55,140
+fixative pure علشان تكون جودته عالية ويثبتلي
+
+391
+00:30:55,140 --> 00:30:58,480
+الخلايا ويعملي preserve لل morphology بشكل جيد
+
+392
+00:31:03,360 --> 00:31:07,400
+طبعا أنا بكون حاطة هذا الكحول في جار في مرتبان
+
+393
+00:31:07,400 --> 00:31:12,300
+زجاجي و هذا المرتبان فيه فرزات لإنه تجلس الشريحة
+
+394
+00:31:12,300 --> 00:31:16,620
+فيه فبالتالي و المرتبان بيكون بيستوعب أكتر من
+
+395
+00:31:16,620 --> 00:31:21,880
+شريحة فبحط الشريحة فيه و بحسب من دقيقتين إلى تلت
+
+396
+00:31:21,880 --> 00:31:26,100
+دقائق و بعدين بطلعها و بخسلها بعد هيك بالماء طبعا
+
+397
+00:31:26,400 --> 00:31:32,240
+لكن قبل لأ ممنوع و الشريحة يلامسها ماء و ممنوع
+
+398
+00:31:32,240 --> 00:31:34,560
+ايضا ان انا انفخ عليها عشان مايجيش بخار الماء
+
+399
+00:31:34,560 --> 00:31:37,840
+عليها و ايضا كمان ممنوع ان انا استخدم fixative
+
+400
+00:31:37,840 --> 00:31:44,110
+سارله تخفيف او يلامسه الماء تمام؟بعد التخفيق ..
+
+401
+00:31:44,110 --> 00:31:47,390
+بعد ال fixation مش مشكلة لو أنا غسلت بالماء طيب
+
+402
+00:31:47,390 --> 00:31:50,710
+هنا بيحكيلي in humid climates it might necessary
+
+403
+00:31:50,710 --> 00:31:54,630
+to replace the methanol 2 to 3 times per day هلأ
+
+404
+00:31:54,630 --> 00:31:58,270
+بيحكيلي انه .. انه في ال .. في الأجواء الرطبة يفضل
+
+405
+00:31:58,270 --> 00:32:01,470
+انه إذا احنا بنشتغل يعني و في blood films كتير
+
+406
+00:32:01,470 --> 00:32:05,270
+بتيجي على المختبر و بدنا نحضرها و نصبغها فبالتالي
+
+407
+00:32:05,270 --> 00:32:07,890
+لازم انه ال methanol يتغير من مرتين لتلاتة في
+
+408
+00:32:07,890 --> 00:32:12,700
+اليوملأنه في هاي الأجواء أو في هاي ال .. ال ..
+
+409
+00:32:12,700 --> 00:32:16,840
+المناخ الرطب ال methanol ممكن يمتص رطوبة و ممكن
+
+410
+00:32:16,840 --> 00:32:21,440
+يصير له dilution أو انه يصير فيه وصله ميه من رطوبة
+
+411
+00:32:21,440 --> 00:32:25,540
+الجو فبالتالي هذا يأثر على عملية ال fixation تمام؟
+
+412
+00:32:26,410 --> 00:32:32,950
+ممكن القديم اللي أنا بدي أستبدله يعني ممكن أستخدمه
+
+413
+00:32:32,950 --> 00:32:39,270
+علشان أنظف فيه الخلايا خصوصا لو كانت الخلايا يعني
+
+414
+00:32:39,270 --> 00:32:45,170
+مثلا عليها الشمع، المادة الشمعية أو عليها زيت أو
+
+415
+00:32:45,170 --> 00:32:49,530
+حتى كمان يعني أنه يعني عليها أي artifact أو
+
+416
+00:32:49,530 --> 00:32:50,430
+contamination
+
+417
+00:32:52,970 --> 00:32:56,070
+it's important to prevent contact with water
+
+418
+00:32:56,070 --> 00:33:01,130
+before fixation is complete طبعا ممنوع هنا ممنوع
+
+419
+00:33:01,130 --> 00:33:04,970
+to prevent contact with water ممنوع الماء يلامس
+
+420
+00:33:04,970 --> 00:33:07,730
+الشريحة قبل ال fixation حكينا بعد ال fixation مش
+
+421
+00:33:07,730 --> 00:33:11,810
+مشكلة لكن قبل ال fixation ممنوع ملامسة الماء ممنوع
+
+422
+00:33:11,810 --> 00:33:14,330
+ال methanol او ال ethanol absolute اللي انا بدى
+
+423
+00:33:14,330 --> 00:33:19,980
+استخدمه يكون عليه ماء تمام؟ليش؟ لإنه the presence
+
+424
+00:33:19,980 --> 00:33:23,380
+of water during methanol fixation produce
+
+425
+00:33:23,380 --> 00:33:26,720
+refractile body artifact water spot in the
+
+426
+00:33:26,720 --> 00:33:31,060
+erythrocyte تمام؟ هذا رح يقديلي إلى artifact إلى
+
+427
+00:33:31,060 --> 00:33:35,090
+أشياء داخلة على ال RBC أزاي كأنها هيك spotنقاط
+
+428
+00:33:35,090 --> 00:33:39,870
+لامعة هذه النقاط اللامعة ممكن إنها تعيق عملية
+
+429
+00:33:39,870 --> 00:33:44,170
+الرؤية ممكن إنها اتشتت الشخص اللي بيقرأ أو بيقيم
+
+430
+00:33:44,170 --> 00:33:48,090
+ال blood film وممكن أيضا إنها تتداخل في ال
+
+431
+00:33:48,090 --> 00:33:52,270
+diagnosis لإن أحيانا أنا بكون بدور على spots معينة
+
+432
+00:33:52,270 --> 00:33:59,600
+داخل ال RBCs علشان أشقص مرض معين أويعني أشخص حالة
+
+433
+00:33:59,600 --> 00:34:05,280
+فبالتالي وجود هاي ال .. ال artifact و يكون سببها
+
+434
+00:34:05,280 --> 00:34:10,240
+الماء ممكن يعطيني diagnosis خاطرthis water spot
+
+435
+00:34:10,240 --> 00:34:16,800
+هاي النقاط أو القطرات من الماء بتبقى موجودة حتى
+
+436
+00:34:16,800 --> 00:34:22,520
+بعد ما أصبغ العينة تمام؟ و ممكن كمان بتكون موجودة
+
+437
+00:34:22,520 --> 00:34:27,480
+ممكن أنها cover items ممكن تغطي أشياء يعني تيجي
+
+438
+00:34:27,480 --> 00:34:33,400
+على خلايا أو تيجي على مثلا طفيل أو أي إشي موجود
+
+439
+00:34:33,400 --> 00:34:37,020
+داخل الخلايا أو موجود في الدم و تغطيه
+
+440
+00:34:40,260 --> 00:34:44,060
+أيضا they are distracting the person ممكن تشتت
+
+441
+00:34:44,060 --> 00:34:48,880
+الشخص لإنها لامعة هيك و .. يعني بتشتت الشخص أو
+
+442
+00:34:48,880 --> 00:34:53,760
+تزرج الشخص اللي بيقيم ال .. ال blood film و in
+
+443
+00:34:53,760 --> 00:34:56,580
+some cases the water spit may .. the water spots
+
+444
+00:34:56,580 --> 00:34:59,340
+may interfere with diagnosis و حكينا إنها ممكن
+
+445
+00:34:59,340 --> 00:35:03,020
+تأثر على ال diagnosisto prevent the alcohol from
+
+446
+00:35:03,020 --> 00:35:07,740
+becoming contaminated by absorbed water طبعا علشان
+
+447
+00:35:07,740 --> 00:35:12,620
+امنع الكحول من انه يمتص ماء او رطوبة من الجو او
+
+448
+00:35:12,620 --> 00:35:16,600
+نصيرله contaminated ب .. بماء لازم انه يكون موجود
+
+449
+00:35:16,600 --> 00:35:20,540
+في bottle with tightly fitting stopper و يكون مغطى
+
+450
+00:35:20,540 --> 00:35:26,090
+بشكل كتير منيح andnot left exposed to the
+
+451
+00:35:26,090 --> 00:35:30,490
+atmosphere و أنا مثلا مابنفع أحطه في الجار و أترك
+
+452
+00:35:30,490 --> 00:35:35,610
+الجار مفتوح، لهيك بيمتص رطوبة و بيأثر على الشغل،
+
+453
+00:35:35,610 --> 00:35:38,270
+تمام؟ و خصوصا في ال humid climates، خصوصا في
+
+454
+00:35:38,270 --> 00:35:43,510
+الأجواء الراطبة أو في الرطوبة العاليةتمام اذا هيك
+
+455
+00:35:43,510 --> 00:35:47,310
+احنا خلصنا ال fixation ثبتنا الخلايا واحتفظنا
+
+456
+00:35:47,310 --> 00:35:51,810
+بشكلها ومنعنا وصولها للماء بعد هيك بعد ال fixation
+
+457
+00:35:51,810 --> 00:35:55,370
+غسلناها مش مشكلة بس قبل لأ ممنوع يلامسها ماء او
+
+458
+00:35:55,370 --> 00:35:59,830
+يكون فيه ال methanol او ال ethanol ملوث بالماءطيب
+
+459
+00:35:59,830 --> 00:36:05,070
+بعد هيك بدي أنا أبدأ أصبغ الشريحة أو أصبغ الفيلم
+
+460
+00:36:05,070 --> 00:36:09,790
+بدي أستخدم Romano Whiskey Stains تمام؟ إيش يعني
+
+461
+00:36:09,790 --> 00:36:12,450
+Romano Whiskey Stains؟ هلأ هال Romano Whiskey
+
+462
+00:36:12,450 --> 00:36:16,910
+Stains هي عبارة عن عائلة من الصبغات تمام؟ هي يعني
+
+463
+00:36:16,910 --> 00:36:20,750
+مش صبغة واحدة هي لأ هي مجموعة هو مصطلح يطلق على
+
+464
+00:36:20,750 --> 00:36:24,790
+مجموعة من الصبغات عائلة من الصبغات تمام؟ هاي
+
+465
+00:36:24,790 --> 00:36:31,840
+الصبغات تستخدم لال ..صبغ ال blood film و بتعطيني
+
+466
+00:36:31,840 --> 00:36:36,400
+نتائج very satisfactory تمام؟ و من أمثلة هذه
+
+467
+00:36:36,400 --> 00:36:40,840
+الصبغات اللي تنتمي لعائلة ال رومانووسكستين ال my
+
+468
+00:36:40,840 --> 00:36:45,180
+.. my grand world gemza تمام؟ و هي مهم .. يعني
+
+469
+00:36:45,180 --> 00:36:49,660
+كتير نيحة لل routine work للشغل ال routine ال
+
+470
+00:36:49,660 --> 00:36:54,080
+gemza stain بتستخدم
+
+471
+00:36:54,080 --> 00:36:57,680
+طبعا تعطيني delicate stain يعني تفاصيل جيدة جدا
+
+472
+00:36:59,740 --> 00:37:04,680
+Lishmans تمام؟unleashments هي عبارة عن the
+
+473
+00:37:04,680 --> 00:37:08,520
+simplest method أفضل أو أبسط طريقة مش أفضل أبسط
+
+474
+00:37:08,520 --> 00:37:12,140
+طريقة يعني في .. في أفضل منها بس هي أبسط طريقة و
+
+475
+00:37:12,140 --> 00:37:16,940
+أسهل طريقة تستخدم لتحضير ال blood film و إحنا
+
+476
+00:37:16,940 --> 00:37:20,700
+بنستخدمها لما بيكون ال blood film مطلوب قراته
+
+477
+00:37:20,700 --> 00:37:23,860
+urgently إيش يعني كلمة urgently هتمور معاكم كتير
+
+478
+00:37:23,860 --> 00:37:29,800
+خلال دراستكم التحليل urgently يعني فحص عاجل أو شو
+
+479
+00:37:29,800 --> 00:37:33,770
+يعنيإيجاك يا عجاتك عينة عاجلان لازم انت تقرا إيها
+
+480
+00:37:33,770 --> 00:37:37,590
+لإنه بيكون الشخص يعني حالته حرجة جدا ما بين الحياة
+
+481
+00:37:37,590 --> 00:37:42,070
+و الموت فانت لازم تشتغليله فحوصاته urgently تمام؟
+
+482
+00:37:42,070 --> 00:37:46,110
+يعني طارق او يعني عاجل هاي معنى كلمة urgently
+
+483
+00:37:46,110 --> 00:37:53,050
+تمام؟ و طبعا ليه .. ليش أنا بستخدمها مع ال urgent
+
+484
+00:37:53,050 --> 00:38:00,360
+cases أو ال urgent samples لإنههي الصبغة منها
+
+485
+00:38:00,360 --> 00:38:04,700
+وفيها fixative وصبغة يعني هي 2 في 1 يعني هي الصبغة
+
+486
+00:38:04,700 --> 00:38:08,760
+نفسها ال powder تم adapt ب methanol فانا مابحتاج
+
+487
+00:38:08,760 --> 00:38:12,180
+لخطوط fixation يعني انا وفرت من 2 ل 3 minute في ال
+
+488
+00:38:12,180 --> 00:38:16,700
+fixation فبحط على طول يعني بعد ما افرض العينة
+
+489
+00:38:16,700 --> 00:38:21,320
+واخليها air dry تمام على طول بصبغها بالشمنالليشمن
+
+490
+00:38:21,320 --> 00:38:24,800
+لإنه هي فيها صبغة وفيها ميثانول هتثبت و تصبغ في
+
+491
+00:38:24,800 --> 00:38:28,420
+نفس الوقت عشان هيك احنا بنحكي عنها simple بسيطة و
+
+492
+00:38:28,420 --> 00:38:31,540
+بتنفع لل urgent cases لحالات الطريقة لإنها مابتاخد
+
+493
+00:38:31,540 --> 00:38:34,640
+وقت خصوصا الشغل اللي بيجي at night مثلا يعني في
+
+494
+00:38:34,640 --> 00:38:38,040
+الليل عينا بعتد في الليل بدنا نقرألها blood film
+
+495
+00:38:38,040 --> 00:38:41,180
+أكيد يعني هي طريقة أو تمر يعني المريض بيكون وضع
+
+496
+00:38:41,180 --> 00:38:45,650
+وحرج عشان هيك بعتوهاfield stains صبغة ال field
+
+497
+00:38:45,650 --> 00:38:53,090
+طبعا هو يعني صبغة سريعة تستخدم لال .. لال thin
+
+498
+00:38:53,090 --> 00:38:56,770
+films أو ال thick films of parasites .. malaria ال
+
+499
+00:38:56,770 --> 00:39:00,950
+parasites، تمام؟ تستخدم لتشخيص التفايليات خصوصا
+
+500
+00:39:00,950 --> 00:39:08,200
+الملارياهلأ كيف تشتغل الرومان ويسكستان؟ يعني سواء
+
+501
+00:39:08,200 --> 00:39:12,700
+كانت جمزة أو رايت أو كانت لشمن أو whatever إيش
+
+502
+00:39:12,700 --> 00:39:17,600
+نوعها، تمام؟ إيه كيف تشتغل؟ هلأ طالما إن هي تنتمي
+
+503
+00:39:17,600 --> 00:39:21,900
+لعائلة الرومان وويسكستان إذا هي عبارة عن صبغة
+
+504
+00:39:21,900 --> 00:39:27,660
+مركبة موجود فيها جزء حامضي وجزء قاعدي، تمام؟ الجزء
+
+505
+00:39:27,660 --> 00:39:32,020
+القاعدي بسميه cationic أو basic dye زي البيثيلين
+
+506
+00:39:32,020 --> 00:39:36,450
+blueو أو أحد مشتقات الأكسدة تبعتها اللي هو ال
+
+507
+00:39:36,450 --> 00:39:41,870
+oxidation product للميثيليني بلو زي ال Azorbi طبعا
+
+508
+00:39:41,870 --> 00:39:46,490
+سواء كانت ميثيليني بلو أو Azorbi طبعا هي صبغة
+
+509
+00:39:46,490 --> 00:39:51,590
+قاعدية cationic طبعا يعني شحنتها موجبة فإذا هي
+
+510
+00:39:51,590 --> 00:39:56,070
+هتصبغ ال anionic أو ال .. ال .. ال acidic أو
+
+511
+00:39:56,070 --> 00:40:01,400
+الأجزاء اللي شحنتها سالبة في الخليةتمام؟ إيش اللي
+
+512
+00:40:01,400 --> 00:40:04,140
+هي الأجزاء الحامضية أو الأجزاء اللي شحنتها سالبة
+
+513
+00:40:04,140 --> 00:40:09,560
+في الخلية؟ مافيش أشهر من الأحماض النووية لل
+
+514
+00:40:09,560 --> 00:40:13,780
+nucleic acid ال DNA و ال RNA فبتصبغهم و بتعطيهم
+
+515
+00:40:13,780 --> 00:40:17,400
+اللون ماباني blue ل grey تمام؟ أحيانا بتعطيهم
+
+516
+00:40:17,400 --> 00:40:22,380
+اللون ال violet تمام؟أيضًا للـ nucleoprotein،
+
+517
+00:40:22,380 --> 00:40:28,480
+بروتينات النواة وحبيبات ال bezophil برضه كمان
+
+518
+00:40:28,480 --> 00:40:33,000
+بتنصبغ فيها و أحيانًا يعني بشكل ضعيف جدًا حبيبات
+
+519
+00:40:33,000 --> 00:40:36,580
+ال neutrophil بتاخد هاي الصبغة طيب، هلأ إيش يعني
+
+520
+00:40:36,580 --> 00:40:39,300
+حبيبات ال bezophil؟ مش إحنا حكينا إنه ال
+
+521
+00:40:39,300 --> 00:40:43,170
+neutrophil و ال bezophil و ال xenophilتمام هدول
+
+522
+00:40:43,170 --> 00:40:47,830
+التلت خلايا بنحكي عنهم granulocyte يعني خلايا
+
+523
+00:40:47,830 --> 00:40:51,870
+محببة الcytoplasm تبعهم فيهم حبيبات؟ اه طيب
+
+524
+00:40:51,870 --> 00:40:56,650
+الbezofilish ليش سميناها أصلا bezofil لإن حبيباتها
+
+525
+00:40:56,650 --> 00:41:04,200
+عبارة عن حبيبات محبةلل basic dye، تمام؟ إذا هي
+
+526
+00:41:04,200 --> 00:41:08,460
+أخدت اسمها من ظهورها على ال blood film، يعني ليش
+
+527
+00:41:08,460 --> 00:41:12,200
+صمينها basophil؟ لأنها تحتوي على حبيبات، phil يعني
+
+528
+00:41:12,200 --> 00:41:15,780
+محبة، تمام؟ جاي من كلمة philic، محبة، لما نقول
+
+529
+00:41:15,780 --> 00:41:21,070
+hydrophilic، محبة للماء، تمام؟ phil محبةو Bezo
+
+530
+00:41:21,070 --> 00:41:26,250
+محبة للقاعدة، اذا حبيباتها تركيبها حامض محبة
+
+531
+00:41:26,250 --> 00:41:31,890
+للصبغة القاعدية اللي هي ال basic dye اللي هي ال
+
+532
+00:41:31,890 --> 00:41:38,140
+methylene blueتمام؟ طيب، وإذاً هل هذا هو المكون
+
+533
+00:41:38,140 --> 00:41:42,460
+الوحيد للصبغة؟ لأ، في مكون آخر بيكون موجود فيها
+
+534
+00:41:42,460 --> 00:41:47,360
+اللي هو الشق الحامضي أو ال anionic أو اللي هو سالب
+
+535
+00:41:47,360 --> 00:41:52,420
+الشحنة زي ال Eocin Y و Eocin B مثال على هذا الشق
+
+536
+00:41:52,420 --> 00:41:57,280
+أو النوع اللي هي صبغة Eocin Y و Eocin Bطبعا هدى
+
+537
+00:41:57,280 --> 00:42:03,940
+الشق أو هدى الجزء او تكوين من الصبغة بيصبغلى ال
+
+538
+00:42:03,940 --> 00:42:10,500
+cationic طبعا هو سالب فهيربط او يرتبط او يصبغ
+
+539
+00:42:10,500 --> 00:42:16,760
+الأجزاء اللى شحنتها موجة بقى زى ال hemoglobin و
+
+540
+00:42:16,760 --> 00:42:19,740
+الazinophilic granule و هيعطيها اللون ما بين ال
+
+541
+00:42:19,740 --> 00:42:25,070
+orange لل red تمام؟طيب ال azinophil لgranule نفس
+
+542
+00:42:25,070 --> 00:42:29,950
+فكرة ال bezophil، هلأ ال azinophil هي خلية
+
+543
+00:42:29,950 --> 00:42:34,290
+granulocyte، في في ال cytoplasm تبعها حبيبات،
+
+544
+00:42:34,290 --> 00:42:39,890
+الحبيبات هادي، phil لمين؟ محب لمين؟ لل-eosin اللي
+
+545
+00:42:39,890 --> 00:42:44,230
+هي لل acidic dye عشان هيكسميناها azinophil granule
+
+546
+00:42:44,230 --> 00:42:49,040
+أو azinophil cellطمام؟ إذا الصبغة بتكون موجود فيها
+
+547
+00:42:49,040 --> 00:42:53,240
+يعني سواء كانت Gemza, Wright, Solishman, Field أي
+
+548
+00:42:53,240 --> 00:42:56,520
+نوع من هذه الصبغات هيكون في methylene blue أو أحد
+
+549
+00:42:56,520 --> 00:42:59,920
+مشتقات الأكسيدات تبعتها زي ال AzorB و هيكون فيها
+
+550
+00:42:59,920 --> 00:43:05,900
+Acy Y و Acy B ال methylene blue هو قاعدي و شحنته
+
+551
+00:43:05,900 --> 00:43:10,060
+موجبة هيصبغ الأشياء السالبة في الشحنة اللي هي
+
+552
+00:43:10,060 --> 00:43:13,480
+الحامضية زي ال DNA و ال RNA و ال nucleoprotein
+
+553
+00:43:14,420 --> 00:43:20,660
+حبيبات ال .. ال .. ال .. بيزوفل تمام؟ طيب أما ال
+
+554
+00:43:20,660 --> 00:43:24,860
+.. الجزء الحامدى اللى هو سالب الشحنة ال anionic زى
+
+555
+00:43:24,860 --> 00:43:30,560
+ال EUCY و EUCB هيرتبط أو يصبغ بال cationic site او
+
+556
+00:43:30,560 --> 00:43:34,320
+اللى هو ايه يعني الأجزاء القاعدية الموجبة الشحنة
+
+557
+00:43:34,320 --> 00:43:37,830
+في الخلية ويعطيها اللون ال orange لل redزي الـ
+
+558
+00:43:37,830 --> 00:43:41,010
+hemoglobin اللى موجود فى ال RBCs هو قاعر يرتبط بال
+
+559
+00:43:41,010 --> 00:43:44,870
+.. ينصبغ بالإيسين و أيضا الازينوفيل ال granule
+
+560
+00:43:44,870 --> 00:43:49,310
+حبيبات الازينوفيل طيب حبيبات للنيوتروفيل من اسمها
+
+561
+00:43:49,310 --> 00:43:53,550
+neutral متعادلة و في المحبة يعنى بتاعها بتنصبغ
+
+562
+00:43:53,550 --> 00:43:56,830
+شوية بال methylene blue و شوية بالازين عشان هيك
+
+563
+00:43:56,830 --> 00:44:00,130
+بتكون لونها دايما حبيبات للنيوتروفيل بيكون لونها
+
+564
+00:44:00,130 --> 00:44:04,630
+pink ماخدة ال .. ده ..يعني خليط من اللون ال blue
+
+565
+00:44:04,630 --> 00:44:08,950
+مع اللون ال orange ل red هذا فبتعطيني اللون ال
+
+566
+00:44:08,950 --> 00:44:13,510
+pink طبعا لازم انا استخدم buffer phosphate buffer
+
+567
+00:44:13,510 --> 00:44:18,050
+اضيفه على الصبغة علشان يؤدي الى تأيينها الشق
+
+568
+00:44:18,050 --> 00:44:22,350
+القاعدى تبعها ياخد الشحنة الموجبة فيصبغل الأجزاء
+
+569
+00:44:22,350 --> 00:44:29,230
+السالبة و الجزء الحامضى ياخد الشحنة السالبة فيصبغل
+
+570
+00:44:29,230 --> 00:44:36,170
+الأجزاء الموجبة في الخليةطبعا هانا ميعطيني طريقة
+
+571
+00:44:36,170 --> 00:44:40,330
+صباغة بالليشمانستان طبعا حاكي ان هين بحضر الاسمير
+
+572
+00:44:40,330 --> 00:44:45,330
+بخليه air-dry على طول بعد ال air-dry بحط الستان
+
+573
+00:44:45,330 --> 00:44:50,150
+ليش؟ لإن الستان هي منها fixative ومنها صبغة في نفس
+
+574
+00:44:50,150 --> 00:44:56,210
+الوقتو بصبغها بعد هيك بخليها تجف و طبعا بحط الصبغة
+
+575
+00:44:56,210 --> 00:44:59,250
+بضيف ال buffer ال buffer بيخلي الصبغة تتأين و تصبغ
+
+576
+00:44:59,250 --> 00:45:03,810
+بتركها من عشر الى خمستاشر دقيقة بغسلها بخليها تجف
+
+577
+00:45:03,810 --> 00:45:08,770
+بعدين بشوفها على ال microscope تمام؟ فإذا احنا
+
+578
+00:45:08,770 --> 00:45:12,410
+هناخد طريقة تانية لإنه هنستخدم الجمزة و هشرحلكم
+
+579
+00:45:12,410 --> 00:45:18,800
+الطريقة بالمختبرطبعا طيب إيش الألوان اللي هتاخدها
+
+580
+00:45:18,800 --> 00:45:22,640
+أجزاء الخلايا؟ طبعا إحنا حكينا أنوية الخلايا موجود
+
+581
+00:45:22,640 --> 00:45:28,560
+فيها DNA و RNA و Nuclear Protein فهتاخد الصبغة
+
+582
+00:45:28,560 --> 00:45:32,320
+القاعدية اللي هي ال methylene blue أو ال azorbia
+
+583
+00:45:32,320 --> 00:45:36,380
+فهتكون مخدة اللون ال purple حكيتلكم هي إما violet
+
+584
+00:45:36,380 --> 00:45:40,470
+أو ال blue فهتكون مخدة اللون ال purpleالـ
+
+585
+00:45:40,470 --> 00:45:44,010
+cytoplasm الـ cytoplasmal erythrocyte فيه
+
+586
+00:45:44,010 --> 00:45:47,110
+هموجلوبيين خلايا الدم الحمرا الـ cytoplasm تبعها
+
+587
+00:45:47,110 --> 00:45:52,110
+مليان هموجلوبيين فهتاخد ال Eucine فهي ينصبغ بال
+
+588
+00:45:52,110 --> 00:45:58,590
+Eucine وتاخد اللون ال Red تمام ال neutrophil ال
+
+589
+00:45:58,590 --> 00:46:03,230
+cytoplasm تبعها موجود في حبيبات و الحبيبات هذه
+
+590
+00:46:03,230 --> 00:46:07,550
+متعادلة فهتاخد ال blue بقدر ما يعني ال methylene
+
+591
+00:46:07,550 --> 00:46:15,000
+blueبقدر ما هتنسبق بال أيوسين Y أو ال .. ال أيوسين
+
+592
+00:46:15,000 --> 00:46:19,860
+دي فبالتالي هيكون لونها ما بين ال orange ل ال pink
+
+593
+00:46:19,860 --> 00:46:23,800
+طبعا ال lymphocytes ال cytoplasm تبعها هيكون blue
+
+594
+00:46:23,800 --> 00:46:28,440
+يعني انسبق بال مثيلين blue تمام ال monocyte هيكون
+
+595
+00:46:28,440 --> 00:46:31,660
+برضه كمان ال cytoplasm تبعها blue و ال mesophil
+
+596
+00:46:31,660 --> 00:46:35,160
+هيكون ال cytoplasm تبعها blueالحبيبات، الحبيبات لل
+
+597
+00:46:35,160 --> 00:46:38,480
+neutral fill حكينا هيكون لونها fine purple أو يعني
+
+598
+00:46:38,480 --> 00:46:43,140
+موف فاتح أو pink، حكينا ليش؟ وال isenophile
+
+599
+00:46:43,140 --> 00:46:47,320
+حبيباتها هتكون مخدة صبغة ال eosin اللي هي red ل
+
+600
+00:46:47,320 --> 00:46:52,060
+orange، ال basophil مخدة صبغة ال basic اللي هي لون
+
+601
+00:46:52,060 --> 00:46:58,360
+ال .. ال ..ال .. ال methylene blue هتكون مخدة
+
+602
+00:46:58,360 --> 00:47:01,680
+اللون اللي ال blue او ال purple او من كتر ما هي
+
+603
+00:47:01,680 --> 00:47:05,320
+bezofil هتكون مخدة ال purple كتير لدرجة انها صارت
+
+604
+00:47:05,320 --> 00:47:11,060
+black .. black تمام؟ ال monocyte هيكون اللي طبعا
+
+605
+00:47:11,060 --> 00:47:14,920
+فيها granules و ما تاخد fine reddish او azerophil
+
+606
+00:47:14,920 --> 00:47:18,950
+طب .. طب لحظةاحنا مش نصنفنا انه ال monocyte و ال
+
+607
+00:47:18,950 --> 00:47:22,370
+lymphocyte هي عبارة عن اي granulocyte يعني مافيش
+
+608
+00:47:22,370 --> 00:47:26,690
+فيها حبيبات شوفوا هو لما صنفوهم حكوا انه ال white
+
+609
+00:47:26,690 --> 00:47:31,010
+species يعني نوعين granulocyte اللي هم ال
+
+610
+00:47:31,010 --> 00:47:34,420
+neutrophil و الesenophil و الbesophilو ال a
+
+611
+00:47:34,420 --> 00:47:37,260
+-granulocyte اللي هما ال monocyte و ال lymphocyte
+
+612
+00:47:37,260 --> 00:47:41,200
+طيب و بعد التصنيف هذا اكتشفوا انه ال monocyte فيها
+
+613
+00:47:41,200 --> 00:47:45,620
+granule ناعمة جدا بنسميها azorophilic granule و
+
+614
+00:47:45,620 --> 00:47:49,220
+هذه بتاخد اللون ال reddish او بتاخد الصبغة اللي هي
+
+615
+00:47:49,220 --> 00:47:55,490
+الصبغة ال a و cطبعا و كيف ال monocyte طبعا بتتحول
+
+616
+00:47:55,490 --> 00:48:00,350
+ل macrophage و بتلتهم و .. و ال .. ال microbes و
+
+617
+00:48:00,350 --> 00:48:04,690
+بتقتلها لازم يكون في عندها حبيبات لإنه يعني العدة
+
+618
+00:48:04,690 --> 00:48:08,150
+و العتاد و أسلحة القتل بتكون مخزنة في الحبيبات هذه
+
+619
+00:48:08,150 --> 00:48:11,630
+ال platelet بيكون فيها حبيبات و مخد اللون ال
+
+620
+00:48:11,630 --> 00:48:17,100
+purpleتمام؟ هاي هنا الخلايا طبعا الخلايا لل
+
+621
+00:48:17,100 --> 00:48:20,800
+neutral fill و الazino fill و ال basophil النواة
+
+622
+00:48:20,800 --> 00:48:25,140
+تبعتهم بتكون segmented تمام؟ بتكون مقسمة و بي ..
+
+623
+00:48:25,140 --> 00:48:31,960
+بيربط ما بينها هيك زي loop تمام؟ يعني ..زي هيك
+
+624
+00:48:31,960 --> 00:48:38,240
+اختناق طبعا طيب جبل تنضج الخلية و بتتقسم النواة
+
+625
+00:48:38,240 --> 00:48:43,780
+بتكون النواة على شكل C-shape زي هيك بهذا الشكل و
+
+626
+00:48:43,780 --> 00:48:48,580
+مش مقسمة فبنسميها band سل طبعا ال neutral فيل إلها
+
+627
+00:48:48,580 --> 00:48:52,660
+band الisinofil إلها band و الbesophil إلها band
+
+628
+00:48:52,750 --> 00:48:56,970
+تمام؟ يعني لازم الخلية أثناء .. قبل ندوجها .. احنا
+
+629
+00:48:56,970 --> 00:48:59,790
+حكينا أنه الـ neutrophil والازينوفل والبيزوفل
+
+630
+00:48:59,790 --> 00:49:06,870
+النواة تبعتها بتكون مقسمة وفي مابينها هيك زي .. زي
+
+631
+00:49:06,870 --> 00:49:12,170
+loop بيربط .. بيربط مابين القطع هذه المقسمةطب جابل
+
+632
+00:49:12,170 --> 00:49:17,630
+ما تتجسم كيف كانت النواة كانت band طبعا لما ندجت
+
+633
+00:49:17,630 --> 00:49:24,410
+الخلية و طلعت من البون مارو على الدم فصارت
+
+634
+00:49:24,410 --> 00:49:30,090
+segmented طبعا فإذا في عندي band neutrophil, band
+
+635
+00:49:30,090 --> 00:49:34,090
+eosinophil, band besophil وفي عندي segmented
+
+636
+00:49:34,090 --> 00:49:37,350
+neutrophil segmented eosinophil, segmented
+
+637
+00:49:37,350 --> 00:49:42,770
+besophil طبعاطيب، هلأ نشوف الـ neutrophil العدد
+
+638
+00:49:42,770 --> 00:49:47,630
+الـ segments للنواء بيتروح ما بين 2 ل 5 طبعا، في
+
+639
+00:49:47,630 --> 00:49:54,470
+عندي من 2 ل 5 loops طبعا، و الحبيبات طبعتها لونها
+
+640
+00:49:54,470 --> 00:49:59,690
+pink و زي ما انتوا شايفين هي خلية كبيرة و ال .. ال
+
+641
+00:49:59,690 --> 00:50:03,650
+cytoplasm طبعها بيكون هيك لونه على pink برضه جاي
+
+642
+00:50:03,650 --> 00:50:06,750
+طيب، ال azinophil .. ال azinophil زي ما انتوا
+
+643
+00:50:06,750 --> 00:50:12,900
+شايفينبتكون النواة غالبا من اتنين لاربعة عدد ال
+
+644
+00:50:12,900 --> 00:50:15,620
+loops تبعتها و ال most common انه يكونوا اتنين
+
+645
+00:50:15,620 --> 00:50:20,680
+مرتبطين مع بعض هم البعض زي عاملين زي شكل النضارة و
+
+646
+00:50:20,680 --> 00:50:25,220
+الحبيبات لونها red و كبيرة، تمام؟ فهذه بسميها a
+
+647
+00:50:25,220 --> 00:50:29,000
+xenophil طيب ال bezophil kid بتكون .. بتكون ايضا
+
+648
+00:50:29,280 --> 00:50:33,020
+فيها two loops هايهم two loops غالبا ال most
+
+649
+00:50:33,020 --> 00:50:37,040
+common two loops تمام؟ و بتكون عليها حبيبات كبيرة
+
+650
+00:50:37,040 --> 00:50:40,400
+تمام؟ زي ما انتوا شايفين هذه الحبيبات من ال black
+
+651
+00:50:40,400 --> 00:50:46,300
+ل purple لونها و بيكون لونها ذامج و بنسميها او او
+
+652
+00:50:46,300 --> 00:50:49,500
+يعني بتكون مخدة شكل الجلاء النوع بتكون مخدة شكل
+
+653
+00:50:49,500 --> 00:50:54,820
+glass shape و بنحكي عن الحبيبات obscure nucleus اش
+
+654
+00:50:54,820 --> 00:50:56,760
+يعني obscure nucleus؟
+
+655
+00:50:58,980 --> 00:51:03,420
+هنا نحكيها basophil
+
+656
+00:51:03,420 --> 00:51:10,100
+granules .. granules
+
+657
+00:51:10,100 --> 00:51:17,280
+تمام obscure nucleus
+
+658
+00:51:20,410 --> 00:51:25,470
+تمام؟ obscure يعني أنها بتغطي النواة لدرجة أنه
+
+659
+00:51:25,470 --> 00:51:29,450
+احنا مش .. مش قادرين نشوف تفاصيل النواة تمام؟ لكن
+
+660
+00:51:29,450 --> 00:51:32,490
+احنا لو .. لو مافيش granules تكون .. هتكون النواة
+
+661
+00:51:32,490 --> 00:51:35,250
+عبارة ال most common أنها تكون two loops بس ممكن
+
+662
+00:51:35,250 --> 00:51:39,570
+تكون من اتنين لاربعة زي ال azenote تمام؟ و بيكونوا
+
+663
+00:51:39,570 --> 00:51:42,870
+مرتبط .. متصلين مع بعضهم البعض هيك بخطرة فيه و
+
+664
+00:51:42,870 --> 00:51:46,390
+عاملين زي شكل النضارة تمام؟ glass chip ال nucleus
+
+665
+00:51:46,390 --> 00:51:50,970
+تمام؟طيب إذا هى الـ neutrophil والإيزينوفل
+
+666
+00:51:50,970 --> 00:51:53,490
+والبيزوفل خلايا محببة زى ما أنتوا شايفين الـ
+
+667
+00:51:53,490 --> 00:51:57,410
+cytoplasm تبعها في حبيبها طيب نجى للـ agranulocyte
+
+668
+00:51:57,410 --> 00:52:00,190
+اللى هى الخلايا الغير محببة أو على الأقل الـ
+
+669
+00:52:00,190 --> 00:52:05,830
+monocyte احنا حكينا بنمشيها على انها هىأجران
+
+670
+00:52:05,830 --> 00:52:09,410
+يولوسايت ليش لإن الحبيبات تبعتها بتكونوش واضحة إلا
+
+671
+00:52:09,410 --> 00:52:11,610
+لازم أشوفها على electron microscope يعني على
+
+672
+00:52:11,610 --> 00:52:15,790
+microscope كتير قوة تكبيره عالية تمام يعني في ال
+
+673
+00:52:15,790 --> 00:52:18,050
+light microscope ال microscope العادي اللي إحنا
+
+674
+00:52:18,050 --> 00:52:22,030
+بنستخدمه صعب أشوف حبيبات ال ال monocyte عشان هيك
+
+675
+00:52:22,030 --> 00:52:25,930
+ممكن نحكي عنها تجاوزا إنها هي أجران يولوسايت لكن
+
+676
+00:52:25,930 --> 00:52:30,500
+اللي بديلكم تعرفوا إنه لأ هي فيها حبيباتطيب ما
+
+677
+00:52:30,500 --> 00:52:33,660
+علينا ال lymphocyte هي عبارة عن خلية صغيرة small
+
+678
+00:52:33,660 --> 00:52:40,540
+white pieces و النواة تبعتها round or oval تمام
+
+679
+00:52:40,540 --> 00:52:46,340
+بتكون إما دائرية أو بيضاوية يعني مقاربة لحجم ال
+
+680
+00:52:46,340 --> 00:52:50,130
+RBCsيعني لو انت شفتي خلية وشكاتي انها lymphocyte
+
+681
+00:52:50,130 --> 00:52:53,010
+على طول اطلعي على النواء تبعتها واطلعي على ال RBCs
+
+682
+00:52:53,010 --> 00:52:58,750
+اللي جنبها اذا لاجيتيهم متقاربين في الحجم معناها
+
+683
+00:52:58,750 --> 00:53:02,050
+انها هي على الأغلب lymphocyte ال cytoplasm تبعها
+
+684
+00:53:02,050 --> 00:53:09,650
+بيكون blue و ال grey و بتكون طبعا ال نواء شغل حجم
+
+685
+00:53:09,650 --> 00:53:14,320
+كبيرمن ال cytoplasm لدرجة انه بس عندي حلقة رفيعة
+
+686
+00:53:14,320 --> 00:53:17,180
+من ال cytoplasm شايفين هنا حلقة رفيعة من ال
+
+687
+00:53:17,180 --> 00:53:21,060
+cytoplasm هي اللي بتكون مبينة بنسميها thin rim of
+
+688
+00:53:21,060 --> 00:53:28,360
+cytoplasm اذا نيجي هنا تاني ال lymphocyte
+
+689
+00:53:28,360 --> 00:53:36,780
+lymphocyte has thin rim of
+
+690
+00:53:39,550 --> 00:53:40,590
+cytoplasm
+
+691
+00:53:43,420 --> 00:53:47,700
+طبعا فإذا ال lymphocyte لها هيك زي حلقة بسيطة من
+
+692
+00:53:47,700 --> 00:53:52,280
+ال cytoplasm بتكون موجودة لإن النواة محتلة معظم
+
+693
+00:53:52,280 --> 00:53:56,380
+حجم الخلية طبعا زي ما انتوا شايفين هنا هذه اللي هي
+
+694
+00:53:56,380 --> 00:53:59,480
+ال lymphocyte طب نيجي ل ال monocyte ال monocyte
+
+695
+00:53:59,480 --> 00:54:03,420
+غالبا بتكون ال kidney .. sorry النواة تبعتها
+
+696
+00:54:03,420 --> 00:54:06,960
+kidney shape طبعا زي شكل الكيلة زي شكل ال kidney
+
+697
+00:54:06,960 --> 00:54:09,640
+shape أو حدوة الفرس ولكن
+
+698
+00:54:11,840 --> 00:54:17,380
+طيب و لكن ال .. ال .. ممكن أنا أشوف monocyte
+
+699
+00:54:17,380 --> 00:54:21,020
+النواء تبعتها شكلها irregular يعني عادي برضه كمان
+
+700
+00:54:21,020 --> 00:54:24,880
+بيكون شكلها غير منتظم بعرفها إنها عبارة عن خلية
+
+701
+00:54:24,880 --> 00:54:29,440
+كتير كبيرة و النواء تبعتها غير منتظمة و
+
+702
+00:54:29,440 --> 00:54:35,130
+الcytoplasm تبعها فيه زي الغباشهنا نتيجة وجود ايه
+
+703
+00:54:35,130 --> 00:54:38,590
+مش حاكيه هنا ال Azirophil granule اللي هي مش واضحة
+
+704
+00:54:38,590 --> 00:54:43,130
+على ال ال light microscope طبعا فعشانها مش واضحة
+
+705
+00:54:43,130 --> 00:54:46,990
+بتكون ال cytoplasm مش نقي ولا pure زي مثلا هنا
+
+706
+00:54:46,990 --> 00:54:51,630
+طبعا فبيكون زي الغباش فيه هذا بسبب وجود ال
+
+707
+00:54:51,630 --> 00:54:56,660
+azirophil granule طبعاطيب طب أنا كيف دي أفرق ما
+
+708
+00:54:56,660 --> 00:55:00,500
+بين مثلا ال band و ما .. ما بين ال monocyte أول
+
+709
+00:55:00,500 --> 00:55:05,320
+حاجة في حال .. في حالة ال band الحبيبات إيه بتفرج؟
+
+710
+00:55:05,320 --> 00:55:07,900
+يعني لو كانت حبيباتها pink فهي band .. band
+
+711
+00:55:07,900 --> 00:55:12,160
+neutrophil لو كانت حبيباتها red معناها هي band
+
+712
+00:55:12,160 --> 00:55:16,700
+xenophil و لو كانت حبيباتها purple غامق لا blue لا
+
+713
+00:55:16,700 --> 00:55:21,880
+black فمعناها هي band bisophil تمام؟طيب، هذه شغلة
+
+714
+00:55:21,880 --> 00:55:25,760
+تانية، لكن أنه أنتوا زي .. شايفين النواة نفس الشكل
+
+715
+00:55:25,760 --> 00:55:31,020
+طيب، هلأ لو أنا جيت سكرت ال .. النواة و .. وكملتها
+
+716
+00:55:31,020 --> 00:55:36,440
+بشكل دائري، تمام؟ و رسمت خط وهمي من نهاية النواة
+
+717
+00:55:36,440 --> 00:55:43,110
+إلى ال .. ال .. ل هنا، تمام؟إذا كان الخط هذا هو
+
+718
+00:55:43,110 --> 00:55:48,970
+يعني أكبر من .. من نصف قطر الدائرة اللي أنا سكرتها
+
+719
+00:55:48,970 --> 00:55:54,130
+معناها دي band تمام؟ يعني مثلا بسكر و بحدد المركز،
+
+720
+00:55:54,130 --> 00:55:57,950
+نقطة المركز و برسم لو المسافة أكتر من نصف القطر
+
+721
+00:55:57,950 --> 00:56:03,570
+معناها أنه دي band تمام؟ لكن لو أنا هنا جيت سكرت و
+
+722
+00:56:03,570 --> 00:56:09,010
+رسمت خطتمام و على اعتبار انه مثلا نص القطر هنا ف
+
+723
+00:56:09,010 --> 00:56:13,490
+.. او المركز هنا هيكون فالمسافة اللي مفتوحة هذه
+
+724
+00:56:13,490 --> 00:56:17,450
+الفرزة هي عبارة عن مساوية او اقل من نص .. لا نصف
+
+725
+00:56:17,450 --> 00:56:22,690
+القطر فمعناها انه هذه خلية monocyte او نواة
+
+726
+00:56:22,690 --> 00:56:24,190
+monocyte مش نواة band
+
+727
+00:56:27,180 --> 00:56:31,120
+طيب إذا هيك بتكون تعرفنا على أشكال الخلايا طب
+
+728
+00:56:31,120 --> 00:56:34,860
+البلاتلت احنا حاكيناه اللي بلاتلت هي فتافيت من
+
+729
+00:56:34,860 --> 00:56:39,680
+خلية ال mega-karyocyte صح؟ تمام فال mega-karyocyte
+
+730
+00:56:39,680 --> 00:56:43,400
+هي خلية كبيرة موجودة في البون مارو لما بيتفتفت ال
+
+731
+00:56:43,400 --> 00:56:46,160
+cytoplasm تبعها وبيطلع ع ال circulation بيطلع على
+
+732
+00:56:46,160 --> 00:56:52,460
+هيئة البلاتلت اللي الصفائح أو الصفايحات تمام؟فهذه
+
+733
+00:56:52,460 --> 00:56:56,700
+الحبيبات أو النقاط اللي لونها purple هذه عبارة عن
+
+734
+00:56:56,700 --> 00:57:00,980
+بليتلت هي هنا هذه بليتلت تمام هذه بليتلت هذه
+
+735
+00:57:00,980 --> 00:57:05,520
+بنسميها بليتلت اللي هنا تكون موجودة ما بين ال RBCs
+
+736
+00:57:05,520 --> 00:57:09,080
+فبنسميها بليتلت هذا بليتلت aggregation تجمع من
+
+737
+00:57:09,080 --> 00:57:13,760
+البليتلت طيب ال RBCs اللي هو ال red blood cell زي
+
+738
+00:57:13,760 --> 00:57:19,950
+ما انتوا شايفين خلاياشكلها discotheque قرصي تمام و
+
+739
+00:57:19,950 --> 00:57:23,630
+ممكن تكون أخدت شكل هيكا oval أو إشي من الضغط لكن
+
+740
+00:57:23,630 --> 00:57:26,950
+هي شكلها قرصي أنا لو شيته طلعت عليها حكينا ال RBCs
+
+741
+00:57:28,050 --> 00:57:32,590
+لما أجى أطلع عليها من ال .. ال .. ال .. الجهة
+
+742
+00:57:32,590 --> 00:57:36,250
+العلوية بشوفها discotheque لكن لما أجى أطلع عليها
+
+743
+00:57:36,250 --> 00:57:39,690
+من side view بشوفها biconcave طبعا فإذا انهي ناجيت
+
+744
+00:57:39,690 --> 00:57:43,190
+أطلع عليها من فوق discotheque زي القرص طبعا زي
+
+745
+00:57:43,190 --> 00:57:47,570
+القرص مخدشك للقرص طبعا و فيها homoglobin ال
+
+746
+00:57:47,570 --> 00:57:50,990
+homoglobin يحتل أكتر من تلتينها يعني أكتر من 60%
+
+747
+00:57:50,990 --> 00:57:55,270
+من حجمهاوفي في المص منطقة فاضية هذه المنطقة
+
+748
+00:57:55,270 --> 00:57:59,530
+الفاضية بسميها ال central baller تمام شحوب تمام
+
+749
+00:57:59,530 --> 00:58:03,170
+central baller فهذا ال central baller يحتل طولة
+
+750
+00:58:03,170 --> 00:58:08,330
+الخلية central baller
+
+751
+00:58:08,330 --> 00:58:09,310
+of
+
+752
+00:58:11,880 --> 00:58:17,320
+RBC's طبعا؟ شوفوا إيش يعني؟ Central المركزي بعلر
+
+753
+00:58:17,320 --> 00:58:22,700
+الشحوب كلمة بعلر شحوب طبعا؟ of RBC's طيب ليش أنا
+
+754
+00:58:22,700 --> 00:58:26,820
+سميتها Central بعلر of RBC's؟ طلعوا معايا إحنا هل
+
+755
+00:58:26,820 --> 00:58:29,280
+جيت لما نحكي عن شخص شاحب إيش يعني؟ و الله بنقول و
+
+756
+00:58:29,280 --> 00:58:34,180
+الله دمه ضعيف و هو شاحبتمام؟ يعني مافيش عنده
+
+757
+00:58:34,180 --> 00:58:37,880
+homoglobin كفاية فنفس الاشي المنطقة هذه مافيش فيها
+
+758
+00:58:37,880 --> 00:58:41,660
+homoglobin فبالتالي بسميها شاحبة المنطقة الشاحبة
+
+759
+00:58:41,660 --> 00:58:45,300
+من الخلية لإن هي منطقة مافيش فيها homoglobin ومش
+
+760
+00:58:45,300 --> 00:58:49,020
+ماخدة لون ال homoglobin تمام؟ لكن لما بنحكي عن شخص
+
+761
+00:58:49,020 --> 00:58:53,580
+والله إنه متورد ليش؟ لإنه .. لإن مثلا الدم بيسر
+
+762
+00:58:53,580 --> 00:58:58,450
+عنده نتيجة وجود homoglobin تمام؟فإذا ارتبط دايما
+
+763
+00:58:58,450 --> 00:59:01,670
+مصطلح الشحوب في انه مافيش عنده homoglobin او انه
+
+764
+00:59:01,670 --> 00:59:05,810
+عنده دم مضريف او كذا فنفس الاشي ان هذه المنطقة
+
+765
+00:59:05,810 --> 00:59:10,350
+سميت بالبعلربش بالمنطقة الشاحبة المركزية اللي
+
+766
+00:59:10,350 --> 00:59:17,050
+بتحتال طول الخلية من طولة الحجم نظرا لإنها فراغ مش
+
+767
+00:59:17,050 --> 00:59:21,080
+موجود فيها homoglobinطب إيش موجود فيها؟ موجود فيها
+
+768
+00:59:21,080 --> 00:59:25,060
+cytoplasm ومكونات أخرى للخلية يعني أنزيمات
+
+769
+00:59:25,060 --> 00:59:30,400
+وبروتينات أخرى بتلزم الخلية في حياتها اللي هو خلال
+
+770
+00:59:30,400 --> 00:59:32,760
+ال 120 يوم رحلة حياتها
+
+771
+00:59:37,700 --> 00:59:44,760
+طيب أنا هيك سبقت ال .. ال .. الشريحة و اتعرفت كي
+
+772
+00:59:44,760 --> 00:59:49,740
+يعني .. أصير صار عندي فكرة على ال .. الخلايا و
+
+773
+00:59:49,740 --> 00:59:55,820
+أجزائها و كيف هيكون شكلها بعد ما تاخد الصباغة و ..
+
+774
+00:59:55,820 --> 01:00:00,030
+هلأ بدي أنا أشوفها تحت ال microscopeبدي اجيب ال
+
+775
+01:00:00,030 --> 01:00:06,130
+field على 10x بعدين احول على 100x طبعا ال 100x انا
+
+776
+01:00:06,130 --> 01:00:10,430
+محتاجة ان انا احط قطرة زيت علشان اشوف بشكل واضح
+
+777
+01:00:10,430 --> 01:00:14,770
+طبعا طيب ممكن اعمل differential اش يعني
+
+778
+01:00:14,770 --> 01:00:18,510
+differential يعني اشوف اعد 100 خلية من ال white
+
+779
+01:00:18,510 --> 01:00:24,390
+pieces وكل ما بعيد خلية بعرف بجيب نوعها طبعا او
+
+780
+01:00:24,390 --> 01:00:28,080
+يعني بحدد نوعهافي النهاية بعد ما أكون عديت 100
+
+781
+01:00:28,080 --> 01:00:31,820
+خلية، بدي أشوف من المية هدولة كم كانوا monocyte،
+
+782
+01:00:31,820 --> 01:00:34,200
+كم كانوا neutrophil، أه كم كانوا eosinophil أو
+
+783
+01:00:34,200 --> 01:00:38,520
+bezophil أو يعني الخلايا الأخرى، فبالتالي هيك بكون
+
+784
+01:00:38,520 --> 01:00:42,220
+أنا عملت differential، جيبت نسبة كل نوع من أنواع
+
+785
+01:00:42,220 --> 01:00:46,740
+ال white species من أصل 100 خلية أنا عديتهم، تمام؟
+
+786
+01:00:47,580 --> 01:00:52,400
+طيب بس انتوا هالده مش .. يعني في المرحلة الحالية
+
+787
+01:00:52,400 --> 01:00:55,120
+مش مطلوب منكم تعملوا differential هتعملوا ان شاء
+
+788
+01:00:55,120 --> 01:00:59,620
+الله بس تروحوا على ال hematology لكن المطلوب منكم
+
+789
+01:00:59,620 --> 01:01:02,720
+حاليا انكوا تكونوا بتعرفوا تجيبوا ال field بتعرفوا
+
+790
+01:01:02,720 --> 01:01:07,500
+ايه الأساسيات الميكروسكوب وكيف تستكشف الشريحة كيف
+
+791
+01:01:07,500 --> 01:01:10,720
+تتعرفي على شكل الخلايا يعني هادي RBCs هادي بلاتلت
+
+792
+01:01:10,720 --> 01:01:13,140
+هادي lymphocyte , monocyte, azenophile,
+
+793
+01:01:13,360 --> 01:01:19,450
+neutrophil, bezophil و هكذا تمام؟طيب أنا بدي أجيب
+
+794
+01:01:19,450 --> 01:01:23,690
+ال field على عشرة طبعا اللي هي مثلا الشريحة تبعتي
+
+795
+01:01:23,690 --> 01:01:28,050
+و عليها ال blood film اللي أنا فردته طبعا لازم أنا
+
+796
+01:01:28,050 --> 01:01:31,410
+لما أعيش معانا أ center الشريحة يعني بدي أحط
+
+797
+01:01:31,410 --> 01:01:35,050
+المنطقة القراءة اللي بدي أقرأها بدي يكون تحتها ال
+
+798
+01:01:35,050 --> 01:01:40,870
+condenser و فوقها مباشرة اللي .. العدسة طبعا؟ طيب
+
+799
+01:01:40,870 --> 01:01:47,030
+فالمنطقة اللي يتقرأ هي منطقة ال .. ال ..ال .. ال
+
+800
+01:01:47,030 --> 01:01:53,030
+.. يعني الطرف الرافيع أو المنطقة ال thin في ال
+
+801
+01:01:53,030 --> 01:01:58,070
+blood smear تمام؟ هذه هي المنطقة اللي أنا بقراها
+
+802
+01:01:58,070 --> 01:02:01,710
+فإذا هذه المنطقة اللي بدي يكون تحتها ال condenser
+
+803
+01:02:01,710 --> 01:02:08,400
+و فوقها العدسةطيب و إذا ب .. بحط عدسة عشرة on و
+
+804
+01:02:08,400 --> 01:02:12,520
+ببدأ أرفع ال stage شوي شوي لحد ما أشوف الخلايا بعد
+
+805
+01:02:12,520 --> 01:02:17,720
+هيك بروح بدور على منطقة في .. يعني على .. يعني ..
+
+806
+01:02:17,720 --> 01:02:21,920
+مكانفي هذه المنطقة انه يكون الخلايا evenly
+
+807
+01:02:21,920 --> 01:02:25,900
+distributed اش يعني evenly distributed؟ يعني انه
+
+808
+01:02:25,900 --> 01:02:32,900
+تكون الخلايا موزعة بشكل منيح و تكون متباعدة شوي و
+
+809
+01:02:32,900 --> 01:02:38,020
+مش يعني مش متراكبة على بعض هالبعض و مافيش زحمة و
+
+810
+01:02:38,020 --> 01:02:42,720
+تكون عشان انا أتعرف على شكل الخلايا بشكل واضح و
+
+811
+01:02:42,720 --> 01:02:50,670
+أصنفها بشكل دقيق تمام؟طيب و بقيم من خلالها ال RBCs
+
+812
+01:02:50,670 --> 01:02:54,990
+distribution شكلها عاملة role information مش عاملة
+
+813
+01:02:54,990 --> 01:02:59,870
+role ال .. الخلايا ال homoglobin تبعها كويس ولا
+
+814
+01:02:59,870 --> 01:03:03,250
+فاضي اذا طبعا كيف ب .. ب .. بحكم اذا ال central
+
+815
+01:03:03,250 --> 01:03:06,170
+barrier هذا محتل اكتر من طولة الخلية معناها انها
+
+816
+01:03:06,170 --> 01:03:08,490
+خلية فاضية و مافيش فيها homoglobin يعني الشخص عنده
+
+817
+01:03:08,490 --> 01:03:14,250
+anemia طيب
+
+818
+01:03:16,450 --> 01:03:21,910
+و بعد هيك بحط قطرة زيت و بحول عالمية و ماتنسوش انه
+
+819
+01:03:21,910 --> 01:03:26,050
+احنا بنفتح القرص في هاي اللحظة علشان انا بدي إضاءة
+
+820
+01:03:26,050 --> 01:03:31,190
+عالية و ببدأ أفحص العينة بشكل systematic بشكل زيك
+
+821
+01:03:31,190 --> 01:03:34,710
+زاج طبعا احنا كمان حكينا انه العد برضه كمان بيكون
+
+822
+01:03:34,710 --> 01:03:38,110
+بشكل زيك زاج اذا بروح من الطرف اللي أنا ببدأ و
+
+823
+01:03:38,110 --> 01:03:44,010
+بفوت لجوة بهذا الشكل طبعا زيك زاجو بكون انا بعد
+
+824
+01:03:44,010 --> 01:03:48,990
+هيك استكشفت الخلية طبعا او استكشفت اشكال الخلايا و
+
+825
+01:03:48,990 --> 01:03:52,830
+اتعرفت عليها ال .. هاي ال bezelfield شايفين؟ two
+
+826
+01:03:52,830 --> 01:03:57,590
+loops طبعا و الحبيبات كبيرة مغطية الأنوية او يعني
+
+827
+01:03:57,590 --> 01:04:00,910
+موجودة .. احيانا بتكون مغطية كل ال .. كل الأنوية
+
+828
+01:04:00,910 --> 01:04:04,850
+ما .. يعني مابيبين تفاصيل النواء منها ال
+
+829
+01:04:04,850 --> 01:04:08,390
+neutrophil حبيباتها ناعم و لونها pink و هاي شايفين
+
+830
+01:04:08,390 --> 01:04:09,370
+هاي أربعة loops
+
+831
+01:04:12,390 --> 01:04:16,210
+ممكن يكونوا خمسة يعني ممكن يكون هذا الخامس هنا
+
+832
+01:04:16,210 --> 01:04:21,470
+isenophile هاي تلاتة loops وحبيبتها لونها أحمر هاي
+
+833
+01:04:21,470 --> 01:04:26,890
+lymphocyte طبعا اللي حكينا إنه في عندي thin rim of
+
+834
+01:04:26,890 --> 01:04:30,150
+cytoplasm وهي هنا ال monocyte اللي هي النواة
+
+835
+01:04:30,150 --> 01:04:38,590
+kidney shape وهنا herpesis تمام؟ بهذا الشكلال RBCs
+
+836
+01:04:38,590 --> 01:04:45,070
+اللي يعني ما بيكون إليها central pattern بتكون
+
+837
+01:04:45,070 --> 01:04:51,530
+spherocyte يعني مخدة شكل كوروي و ليس ديسكوتيك طبعا
+
+838
+01:04:51,530 --> 01:04:58,550
+طيب هلأ أنا إيش بدي أعمل مطلوب مني يعني أعرف إيش
+
+839
+01:04:58,550 --> 01:05:02,730
+فكرة ال differential differential
+
+840
+01:05:02,730 --> 01:05:04,670
+طبعا
+
+841
+01:05:09,560 --> 01:05:13,480
+طيب إيش يعني differential؟ حكينا معنى كلمة
+
+842
+01:05:13,480 --> 01:05:30,880
+differential إنه أنا .. لحظة شوف .. ليش ..
+
+843
+01:05:30,880 --> 01:05:32,140
+ما بعرف ليش ..
+
+844
+01:05:42,090 --> 01:05:46,790
+طبعا، هلأ أنا بدي أروح أجيب يعني و أنا ب .. أبعمل
+
+845
+01:05:46,790 --> 01:05:51,810
+scanning و مسح لل .. لل blood film و بفحصه بشكل
+
+846
+01:05:51,810 --> 01:05:57,190
+zigzag زي هيك، طبعا، بدي أكون بعد ميت خلية white
+
+847
+01:05:57,190 --> 01:06:00,950
+pieces و في نفس الوقت الميت خلية هدول مثلا بدي
+
+848
+01:06:00,950 --> 01:06:05,430
+أكون أنا على الورقام صنفاهم يعني على ال .. ال
+
+849
+01:06:05,430 --> 01:06:08,090
+report اللي .. أو المكان اللي أنا بدي أسجل فيه
+
+850
+01:06:08,090 --> 01:06:10,590
+نتائج تبعتي، مثلا cat بالينفا
+
+851
+01:06:18,200 --> 01:06:25,120
+lympho mono neutro
+
+852
+01:06:25,120 --> 01:06:34,960
+ايو زينو طبعا انا بس باختصر الكلمات على أساس انه
+
+853
+01:06:34,960 --> 01:06:40,620
+ماأطول في الكتابة بس يعني انه lymphocyte,
+
+854
+01:06:40,700 --> 01:06:45,180
+monocyte, neutrophil, xenophil and bisophil تمام؟
+
+855
+01:06:45,330 --> 01:06:50,150
+طيب، هلأ مثلا أنا و أنا بعد لأ وجدت اتنين ..
+
+856
+01:06:50,150 --> 01:06:54,530
+اتنتين lymphocyte بروح بحط مثلا زي هيك تمام، هي
+
+857
+01:06:54,530 --> 01:07:00,490
+عصيتين، مثلا وجدت واحدة monocyte، هي زي هيك تمام
+
+858
+01:07:00,490 --> 01:07:05,150
+ال neutrophil، ماشيت مثلا على field تاني، طبعا أنا
+
+859
+01:07:05,150 --> 01:07:10,740
+بكون بعمل scanning، ماشية، بتحرك، هيكو نزلت و كمان
+
+860
+01:07:10,740 --> 01:07:14,660
+لفت و نزلت و لفت و هكذا تمام؟ أثناء اللعب مثلا
+
+861
+01:07:14,660 --> 01:07:22,580
+وجدت تلاتة lymphocyte فهي .. هاي تلاتة مثلا ال ..
+
+862
+01:07:22,580 --> 01:07:31,600
+وجدت مثلا واحدة xenofel وجدت مثلا zerobezofel رجعت
+
+863
+01:07:31,600 --> 01:07:36,580
+هنا تاني كمان و روحت على field أخر مثلا وجدت أنه
+
+864
+01:07:36,580 --> 01:07:46,410
+في عندي ..مثلا تنتين lymph تنتين mono تنتين
+
+865
+01:07:46,410 --> 01:07:50,590
+neutral زي هيك بروح بحط الرابعة و الخامسة بحطها
+
+866
+01:07:50,590 --> 01:07:55,030
+بشكل مائل يعني بسكر فيها ال .. الأربعة دول كل خمسة
+
+867
+01:07:55,030 --> 01:07:58,810
+.. كل أربعة الخمسة بتسكرهم يعني زي الحزم في
+
+868
+01:07:58,810 --> 01:08:03,840
+النهاية عدد الخلايا هنا بديكون مية خليةو ال
+
+869
+01:08:03,840 --> 01:08:07,920
+lymphocyte في الآخر عدد العصيات اللي أنا حطيتهم أو
+
+870
+01:08:07,920 --> 01:08:13,760
+عدد الخلايا اللي أنا عديتهم هو عبارة عن نسبة
+
+871
+01:08:13,760 --> 01:08:17,560
+المئوية لل lymphocyte لإن هو عدد الخلايا خلال 100
+
+872
+01:08:17,560 --> 01:08:21,820
+خليةطبعا و نفس الاشي للمونو للنيوترو والإيزينو طيب
+
+873
+01:08:21,820 --> 01:08:26,160
+ال .. ال .. الوضع الطبيعي طبعا من .. من تقريبا من
+
+874
+01:08:26,160 --> 01:08:30,860
+15 إلى 20 أو إلى 25 نسبة طبيعية لل lymphocyte ال
+
+875
+01:08:30,860 --> 01:08:36,140
+monocyte مثلا من 8 ل 10% أو ل 15% لل neutral من 60
+
+876
+01:08:36,140 --> 01:08:40,760
+ل 70% هو ال normal .. ال normal ال eizinophil من 1
+
+877
+01:08:40,760 --> 01:08:46,510
+إلى 2%أو حتى كمان أحيانا بيحكوا أنه من 0 إلى 2%
+
+878
+01:08:46,510 --> 01:08:50,450
+لإنه ممكن ما أشوفش ولا إزينو و ال basophil أيضا
+
+879
+01:08:50,450 --> 01:08:55,430
+نفس الأشي من 0 إلى 1 أو إلى 2% برضه كمان لإنه ال
+
+880
+01:08:55,430 --> 01:08:58,990
+basophil يعني كمان برضه كمان نادر لما أشوفها، هلأ
+
+881
+01:08:58,990 --> 01:09:02,890
+ال lymphocyte وظيفتها إنها بتحافظ على الخلق يعني
+
+882
+01:09:02,890 --> 01:09:07,690
+بت .. بتهاجم ال .. ال virusتمام؟ هي بتحافظ على
+
+883
+01:09:07,690 --> 01:09:11,890
+الجسم من ال viruses من ال viral infection ال
+
+884
+01:09:11,890 --> 01:09:15,670
+monocyte بتلتهم الخلايا بتتحول طبعاً ل macrophage
+
+885
+01:09:15,670 --> 01:09:19,610
+و بتروح تلتهم الخلايا الميتة أو الخلايا السرطانية
+
+886
+01:09:19,610 --> 01:09:24,030
+أو حتى ال .. البكتيريا و بتقتلها ال neutrophil بت
+
+887
+01:09:24,030 --> 01:09:27,770
+.. طبعاً هي بتهاجم ضد البكتيريا يعني ضد ال
+
+888
+01:09:27,770 --> 01:09:31,660
+bacterial infectionتمام ال azinophil ضد ال
+
+889
+01:09:31,660 --> 01:09:34,680
+parasite ضد الطفاليات و أيضا إلها دور في ال
+
+890
+01:09:34,680 --> 01:09:38,320
+allergy و ال bisophil كمان إلها دور في ال allergy
+
+891
+01:09:38,320 --> 01:09:42,110
+و في ال antigen representationيعني في تقديم ال
+
+892
+01:09:42,110 --> 01:09:47,210
+antigen للخلايا التانية عشان انها تقتله تمام؟ هذا
+
+893
+01:09:47,210 --> 01:09:52,410
+وظائف الخلايا او حسب يعني .. او تخصصها في الدفاع
+
+894
+01:09:52,410 --> 01:09:57,730
+عن جسم الإنسان طيب لو كان في عندي زيادة في ال
+
+895
+01:09:57,730 --> 01:10:02,050
+lymphocyte ايش بسميها هذا المصطلح؟ بسميه
+
+896
+01:10:02,050 --> 01:10:10,000
+lymphocytetosis تمام lymphocytosis وإذا كانت في
+
+897
+01:10:10,000 --> 01:10:15,160
+عندي طبعا قلة في ال lymphocyte أو نقص في عدد ال
+
+898
+01:10:15,160 --> 01:10:20,900
+lymphocyte فمعناها هذا بسميه lympho
+
+899
+01:10:20,900 --> 01:10:29,400
+lymphocytopenia
+
+900
+01:10:29,400 --> 01:10:35,450
+lymphocytopeniaعلى وزم ليكوبينيا حكينا الليكوبينيا
+
+901
+01:10:35,450 --> 01:10:39,310
+يعني قلة في ال white pieces ككل و اللي .. و ال ..
+
+902
+01:10:39,310 --> 01:10:42,990
+ليكو سايتوسز يعني زيادة في ال white pieces ككل و
+
+903
+01:10:42,990 --> 01:10:49,110
+هاد .. هاد الاشي ذكرناه في محاضرة ال .. ال white
+
+904
+01:10:49,110 --> 01:10:53,010
+pieces، تمام؟ و ذكرناه أيضا في .. في فحص ال
+
+905
+01:10:53,010 --> 01:10:57,890
+homoglobin تمام،
+
+906
+01:10:57,890 --> 01:11:02,650
+monocytosisنفس فكرة ال lymphocyte ما هي اصلا كلمة
+
+907
+01:11:02,650 --> 01:11:06,570
+lymphocyte تنتهي ب site و ال monocyte تنتهي ب site
+
+908
+01:11:06,570 --> 01:11:12,090
+فعشان هم بينتهوا زي بعض فهيكون
+
+909
+01:11:12,090 --> 01:11:16,670
+المصطلح اللي بيعبر عن جامعهم او عن زيادتهم او
+
+910
+01:11:16,670 --> 01:11:20,230
+نقصانهم نفس الاشي مثلا زيادة ال lymphocyte
+
+911
+01:11:20,230 --> 01:11:26,110
+lymphocytosis زيادة ال monocyte monocytosis
+
+912
+01:11:28,340 --> 01:11:38,620
+طبعا نقف ال monocyte هيكون monocytopenia
+
+913
+01:11:38,620 --> 01:11:45,160
+.. monocytopenia طبعا طيب ال .. ال neutral في
+
+914
+01:11:45,160 --> 01:11:51,660
+الزيادتها neutrophilia
+
+915
+01:11:51,660 --> 01:11:56,660
+.. طبعا neutrophilia و نقصانها
+
+916
+01:12:04,910 --> 01:12:08,250
+neutropenia نفس الأشي ال azenophil زيادتها
+
+917
+01:12:08,250 --> 01:12:14,370
+azenophilia ونقصانها azenopenia, bezophilia,
+
+918
+01:12:14,770 --> 01:12:20,550
+bezocytopenia طبعا فبالتالي لازم هذه الشغلات أنتوا
+
+919
+01:12:20,550 --> 01:12:22,890
+تكونوا بتعرفوها أو المصطلحات تكونوا بتعرفوها و
+
+920
+01:12:22,890 --> 01:12:25,570
+تكونوا بتعرفوا إيش فكرة ال difference يعني طبعا
+
+921
+01:12:25,570 --> 01:12:30,030
+لكن أنهفعليا تفاصيل ال differential إيش مطلوب منكم
+
+922
+01:12:30,030 --> 01:12:35,010
+أو كيف أنه تعملوا هذا تاخدوا في ال hematology
+
+923
+01:12:35,010 --> 01:12:43,870
+تمام؟ بهيك بنكون وصلنا لنهاية محاضرتنا لليوم ان
+
+924
+01:12:43,870 --> 01:12:46,570
+شاء الله تكون واضحة وسهلة بالنسبة لإلكم ويعطيكم
+
+925
+01:12:46,570 --> 01:12:47,390
+ألف عافية
+
diff --git a/PL9fwy3NUQKwY9jl8xw-bWkVZkWcGGyspd/ELhdSBMOyuo_raw.json b/PL9fwy3NUQKwY9jl8xw-bWkVZkWcGGyspd/ELhdSBMOyuo_raw.json
new file mode 100644
index 0000000000000000000000000000000000000000..fbd5e257fb93a14d327300f921e951f29f208d8d
--- /dev/null
+++ b/PL9fwy3NUQKwY9jl8xw-bWkVZkWcGGyspd/ELhdSBMOyuo_raw.json
@@ -0,0 +1 @@
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"compression_ratio": 1.0693069306930694, "no_speech_prob": 2.86102294921875e-06, "words": [{"start": 729.93, "end": 730.55, "word": "طيب", "probability": 0.990234375}, {"start": 730.55, "end": 731.45, "word": " occupy", "probability": 0.88427734375}, {"start": 731.45, "end": 732.63, "word": " two", "probability": 0.93212890625}, {"start": 732.63, "end": 733.11, "word": " thirds", "probability": 0.34033203125}, {"start": 733.11, "end": 733.41, "word": " of", "probability": 0.990234375}, {"start": 733.41, "end": 733.55, "word": " the", "probability": 0.89599609375}, {"start": 733.55, "end": 733.87, "word": " total", "probability": 0.86962890625}, {"start": 733.87, "end": 734.31, "word": " slide", "probability": 0.98486328125}, {"start": 734.31, "end": 734.63, "word": " area", "probability": 0.88671875}, {"start": 734.63, "end": 735.17, "word": " تحتل", "probability": 0.8328450520833334}, {"start": 735.17, "end": 735.77, "word": " تقريبا", "probability": 0.98935546875}, {"start": 735.77, "end": 736.25, "word": " تلتان", "probability": 0.9132080078125}, {"start": 736.25, "end": 737.09, "word": " الشريحة", "probability": 0.9814453125}, {"start": 737.09, "end": 737.79, "word": " و", "probability": 0.7490234375}, {"start": 737.79, "end": 738.61, "word": " أيضا", "probability": 0.8787434895833334}], "temperature": 1.0}, {"id": 34, "seek": 76528, "start": 739.7, "end": 765.28, "text": "shouldn't touch any edge of the slide مش لازم تلامس أي طرف أو أي حافة من حواف الشريحة تمام؟ وكمان should be margin free مايكونش فيها فراغات مع ده منطقة القطرة هاي هنا أنا حطيت القطرة و فردت الشريحة مكان القطرة هيصير في عندي زي الفراغ", "tokens": [2716, 429, 77, 380, 2557, 604, 4691, 295, 264, 4137, 37893, 5296, 31377, 2304, 6055, 37440, 3794, 36632, 23032, 28480, 34051, 36632, 11331, 31845, 3660, 9154, 11331, 14407, 5172, 25124, 16572, 5016, 3660, 46811, 10943, 22807, 4032, 24793, 7649, 820, 312, 10270, 1737, 19446, 1829, 30544, 8592, 8978, 11296, 6156, 2288, 5718, 118, 9307, 20449, 11778, 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{"start": 758.34, "end": 758.5, "word": " هنا", "probability": 0.92041015625}, {"start": 758.5, "end": 758.7, "word": " أنا", "probability": 0.6748046875}, {"start": 758.7, "end": 759.08, "word": " حطيت", "probability": 0.9195963541666666}, {"start": 759.08, "end": 759.62, "word": " القطرة", "probability": 0.9890950520833334}, {"start": 759.62, "end": 760.22, "word": " و", "probability": 0.96484375}, {"start": 760.22, "end": 760.94, "word": " فردت", "probability": 0.863525390625}, {"start": 760.94, "end": 761.94, "word": " الشريحة", "probability": 0.99560546875}, {"start": 761.94, "end": 763.1, "word": " مكان", "probability": 0.86279296875}, {"start": 763.1, "end": 763.62, "word": " القطرة", "probability": 0.9933268229166666}, {"start": 763.62, "end": 764.04, "word": " هيصير", "probability": 0.89501953125}, {"start": 764.04, "end": 764.14, "word": " في", "probability": 0.97705078125}, {"start": 764.14, "end": 764.46, "word": " عندي", "probability": 0.845703125}, {"start": 764.46, 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"probability": 0.9691162109375}, {"start": 786.48, "end": 786.6, "word": " ما", "probability": 0.91943359375}, {"start": 786.6, "end": 786.96, "word": " احط", "probability": 0.7855631510416666}, {"start": 786.96, "end": 787.38, "word": " قطرة", "probability": 0.9806315104166666}, {"start": 787.38, "end": 787.82, "word": " الدم", "probability": 0.98583984375}, {"start": 787.82, "end": 788.06, "word": " على", "probability": 0.63720703125}, {"start": 788.06, "end": 788.86, "word": " الشريحة", "probability": 0.992431640625}, {"start": 788.86, "end": 789.52, "word": " لازم", "probability": 0.9842122395833334}, {"start": 789.52, "end": 789.72, "word": " انا", "probability": 0.93359375}, {"start": 789.72, "end": 790.22, "word": " اعمل", "probability": 0.9694010416666666}, {"start": 790.22, "end": 790.78, "word": " الفردة", "probability": 0.9534505208333334}, {"start": 790.78, "end": 791.04, "word": " او", "probability": 0.954833984375}, {"start": 791.04, "end": 791.18, "word": " ال", 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اللازم إن أنا أعمل incubation لل RBCs على 37 درجة أحطها مثلا في ال water bath أو في ال incubator بعدين أدفع العينة بعدين أحضر منها لسمير لايبينيا إذا الشخص عنده دهون", "tokens": [39237, 10943, 22807, 13672, 31377, 2304, 36145, 41850, 5551, 25957, 1211, 33345, 399, 24976, 497, 7869, 82, 15844, 13435, 11778, 47341, 3660, 5551, 5016, 9566, 11296, 50113, 15040, 8978, 2423, 1281, 6079, 34051, 8978, 2423, 33345, 1639, 39182, 9957, 5551, 3215, 5172, 3615, 18863, 9957, 3660, 39182, 9957, 5551, 5016, 11242, 2288, 9154, 11296, 5296, 38251, 13546, 5296, 47302, 3555, 9957, 25528, 11933, 15730, 25124, 9778, 9381, 43242, 3224, 11778, 3224, 11536], "avg_logprob": -0.18985445532080245, "compression_ratio": 1.4450261780104712, "no_speech_prob": 1.1324882507324219e-06, "words": [{"start": 1515.63, "end": 1516.47, "word": "تمام؟", "probability": 0.6580403645833334}, {"start": 1516.47, "end": 1516.95, "word": " اللازم", "probability": 0.9443359375}, {"start": 1516.95, "end": 1517.11, "word": " إن", 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صبغة", "probability": 0.9962158203125}, {"start": 2810.32, "end": 2810.48, "word": " ال", "probability": 0.98095703125}, {"start": 2810.48, "end": 2810.88, "word": " basic", "probability": 0.9794921875}, {"start": 2810.88, "end": 2811.14, "word": " اللي", "probability": 0.981689453125}, {"start": 2811.14, "end": 2811.68, "word": " هي", "probability": 0.93017578125}, {"start": 2811.68, "end": 2812.06, "word": " لون", "probability": 0.846923828125}, {"start": 2812.06, "end": 2812.42, "word": " ال", "probability": 0.822265625}, {"start": 2812.42, "end": 2812.52, "word": " ..", "probability": 0.931640625}, {"start": 2812.52, "end": 2812.88, "word": " ال", "probability": 0.88623046875}, {"start": 2812.88, "end": 2813.26, "word": " ..", "probability": 0.98046875}], "temperature": 1.0}, {"id": 125, "seek": 283634, "start": 2814.82, "end": 2836.34, "text": "ال .. ال methylene blue هتكون مخدة اللون اللي ال blue او ال purple او من كتر ما هي bezofil هتكون مخدة ال purple كتير لدرجة انها صارت black .. black تمام؟ ال monocyte هيكون اللي طبعا فيها granules و ما تاخد fine reddish او azerophil طب .. طب لحظة", "tokens": [6027, 4386, 2423, 36599, 21323, 3344, 8032, 2655, 30544, 3714, 9778, 41891, 13672, 11536, 13672, 1829, 2423, 3344, 1975, 2407, 2423, 9656, 1975, 2407, 9154, 9122, 2655, 2288, 19446, 39896, 312, 4765, 19776, 8032, 2655, 30544, 3714, 9778, 41891, 2423, 9656, 9122, 2655, 13546, 5296, 3215, 47341, 3660, 16472, 11296, 20328, 9640, 2655, 2211, 4386, 2211, 46811, 10943, 22807, 2423, 1108, 31078, 975, 8032, 1829, 30544, 13672, 1829, 23032, 3555, 3615, 995, 8978, 11296, 9370, 3473, 4032, 19446, 6055, 47283, 3215, 2489, 2182, 40974, 1975, 2407, 7883, 260, 5317, 388, 23032, 3555, 4386, 23032, 3555, 5296, 5016, 19913, 3660], "avg_logprob": -0.21031250104308127, "compression_ratio": 1.6572769953051643, "no_speech_prob": 1.7881393432617188e-07, "words": [{"start": 2814.82, "end": 2815.28, "word": "ال", "probability": 0.74853515625}, {"start": 2815.28, "end": 2815.72, "word": " ..", 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loops طبعا، و الحبيبات طبعتها لونها pink و زي ما انتوا شايفين هي خلية كبيرة و ال .. ال cytoplasm طبعها بيكون هيك لونه على pink برضه جاي طيب، ال azinophil .. ال azinophil زي ما انتوا شايفين", "tokens": [9566, 1829, 3555, 12399, 8032, 1211, 10721, 8717, 8592, 38688, 2423, 39184, 7989, 11741, 388, 18863, 3215, 3215, 2423, 39184, 19904, 24976, 1863, 2407, 16606, 4724, 36081, 32887, 5016, 19446, 49374, 568, 5296, 1025, 23032, 3555, 3615, 995, 12399, 8978, 18871, 16254, 9154, 568, 5296, 1025, 16121, 23032, 3555, 3615, 995, 12399, 4032, 21542, 21292, 3555, 9307, 23032, 3555, 34268, 11296, 5296, 11536, 11296, 7022, 4032, 30767, 1829, 19446, 16472, 2655, 14407, 13412, 995, 33911, 9957, 39896, 16490, 1211, 10632, 9122, 3555, 48923, 4032, 2423, 4386, 2423, 3185, 19337, 7743, 76, 23032, 3555, 3615, 11296, 4724, 1829, 30544, 39896, 4117, 5296, 11536, 3224, 15844, 7022, 4724, 43042, 3224, 10874, 47302, 23032, 1829, 3555, 12399, 2423, 7883, 259, 5317, 388, 4386, 2423, 7883, 259, 5317, 388, 30767, 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ممكن تكون من اتنين لاربعة زي ال azenote تمام؟ و بيكونوا مرتبط .. متصلين مع بعضهم البعض هيك بخطرة فيه و عاملين زي شكل النضارة تمام؟ glass chip ال nucleus تمام؟", "tokens": [39237, 10943, 22807, 34443, 37495, 22653, 14739, 11296, 39894, 17082, 9566, 1829, 28239, 14407, 3660, 5296, 3215, 47341, 3660, 14739, 3224, 1975, 5016, 8315, 37893, 4386, 37893, 12174, 18513, 2288, 9957, 8717, 8592, 38688, 6055, 5172, 33546, 26895, 28239, 14407, 3660, 46811, 10943, 22807, 44381, 1975, 5016, 8315, 45164, 4386, 45164, 19446, 41185, 8592, 9370, 3473, 6055, 30544, 4386, 8032, 2655, 30544, 28239, 14407, 3660, 6225, 3555, 9640, 3660, 2423, 881, 2689, 14739, 11296, 6055, 30544, 732, 16121, 4724, 3794, 3714, 43020, 6055, 30544, 9154, 1975, 2655, 1863, 9957, 5296, 9640, 3555, 27884, 30767, 1829, 2423, 257, 2904, 1370, 46811, 10943, 22807, 4032, 4724, 1829, 30544, 14407, 3714, 43500, 3555, 9566, 4386, 44650, 36520, 9957, 20449, 45030, 11242, 16095, 29739, 3615, 11242, 39896, 4117, 4724, 9778, 9566, 25720, 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{"id": 138, "seek": 312261, "start": 3108.41, "end": 3122.61, "text": "طيب إذا هى الـ neutrophil والإيزينوفل والبيزوفل خلايا محببة زى ما أنتوا شايفين الـ cytoplasm تبعها في حبيبها طيب نجى للـ agranulocyte اللى هى الخلايا الغير محببة أو على الأقل الـ monocyte احنا حكينا بنمشيها على انها هى", "tokens": [9566, 1829, 3555, 11933, 15730, 8032, 7578, 2423, 39184, 7989, 11741, 388, 16070, 28814, 1829, 11622, 9957, 38688, 1211, 16070, 21292, 11622, 38688, 1211, 16490, 15040, 25528, 3714, 5016, 3555, 49401, 30767, 7578, 19446, 14739, 2655, 14407, 13412, 995, 33911, 9957, 2423, 39184, 3185, 19337, 7743, 76, 6055, 3555, 3615, 11296, 8978, 11331, 21292, 3555, 11296, 23032, 1829, 3555, 8717, 7435, 7578, 24976, 39184, 623, 4257, 425, 31078, 975, 13672, 7578, 8032, 7578, 33962, 15040, 25528, 6024, 118, 13546, 3714, 5016, 3555, 49401, 34051, 15844, 16247, 4587, 1211, 2423, 39184, 1108, 31078, 975, 1975, 5016, 8315, 11331, 4117, 1829, 8315, 4724, 1863, 2304, 8592, 1829, 11296, 15844, 16472, 11296, 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lymphocyte هي عبارة عن خلية صغيرة small white pieces و النواة تبعتها round or oval تمام بتكون إما دائرية أو بيضاوية يعني مقاربة لحجم ال RBCs", "tokens": [9566, 1829, 3555, 19446, 25894, 8315, 2423, 31070, 31078, 975, 39896, 6225, 3555, 9640, 3660, 18871, 16490, 1211, 10632, 20328, 17082, 48923, 1359, 2418, 3755, 4032, 28239, 14407, 3660, 6055, 3555, 34268, 11296, 3098, 420, 37175, 46811, 10943, 39894, 30544, 11933, 15042, 11778, 16373, 2288, 10632, 34051, 4724, 1829, 11242, 995, 2407, 10632, 37495, 22653, 3714, 4587, 9640, 49401, 5296, 5016, 7435, 2304, 2423, 497, 7869, 82], "avg_logprob": -0.13970588322948008, "compression_ratio": 1.3444444444444446, "no_speech_prob": 3.5762786865234375e-07, "words": [{"start": 3149.4, "end": 3149.86, "word": "طيب", "probability": 0.970703125}, {"start": 3149.86, "end": 3150.5, "word": " ما", "probability": 0.492431640625}, {"start": 3150.5, "end": 3150.88, "word": " علينا", "probability": 0.7122802734375}, {"start": 3150.88, "end": 3151.06, "word": " 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عدد العصيات اللي أنا حطيتهم أو عدد الخلايا اللي أنا عديتهم هو عبارة عن نسبة المئوية لل lymphocyte لإن هو عدد الخلايا خلال 100 خلية", "tokens": [2407, 2423, 31070, 31078, 975, 8978, 6024, 95, 34740, 6225, 3215, 3215, 18863, 9381, 1829, 9307, 13672, 1829, 41850, 11331, 9566, 36081, 16095, 34051, 6225, 3215, 3215, 33962, 15040, 25528, 13672, 1829, 41850, 6225, 16254, 2655, 16095, 31439, 6225, 3555, 9640, 3660, 18871, 8717, 35457, 3660, 9673, 19986, 2407, 10632, 24976, 31070, 31078, 975, 5296, 28814, 1863, 31439, 6225, 3215, 3215, 33962, 15040, 25528, 16490, 1211, 6027, 2319, 16490, 1211, 10632], "avg_logprob": -0.1222873270097706, "compression_ratio": 1.72, "no_speech_prob": 5.960464477539063e-08, "words": [{"start": 4083.44, "end": 4083.6, "word": "و", "probability": 0.9716796875}, {"start": 4083.6, "end": 4083.84, "word": " ال", "probability": 0.71240234375}, {"start": 4083.84, "end": 4084.46, "word": " lymphocyte", "probability": 0.6944986979166666}, {"start": 4084.46, "end": 4084.58, 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"temperature": 1.0}, {"id": 188, "seek": 431666, "start": 4288.34, "end": 4316.66, "text": "طبعا نقف ال monocyte هيكون monocytopenia .. monocytopenia طبعا طيب ال .. ال neutral في الزيادتها neutrophilia .. طبعا neutrophilia و نقصانها", "tokens": [9566, 3555, 3615, 995, 8717, 4587, 5172, 2423, 1108, 31078, 975, 39896, 30544, 1108, 31078, 19337, 268, 654, 4386, 1108, 31078, 19337, 268, 654, 23032, 3555, 3615, 995, 23032, 1829, 3555, 2423, 4386, 2423, 10598, 8978, 6024, 110, 1829, 18513, 2655, 11296, 7989, 11741, 24169, 4386, 23032, 3555, 3615, 995, 7989, 11741, 24169, 4032, 8717, 4587, 9381, 7649, 11296], "avg_logprob": -0.2628906384110451, "compression_ratio": 1.4803149606299213, "no_speech_prob": 5.960464477539063e-08, "words": [{"start": 4288.34, "end": 4288.92, "word": "طبعا", "probability": 0.72747802734375}, {"start": 4288.92, "end": 4290.5, "word": " نقف", "probability": 0.8564453125}, {"start": 4290.5, "end": 4291.4, "word": " ال", "probability": 0.947265625}, {"start": 4291.4, "end": 4292.38, "word": " monocyte", "probability": 0.8505859375}, {"start": 4292.38, "end": 4293.62, "word": " هيكون", "probability": 0.90283203125}, {"start": 4293.62, "end": 4298.62, "word": " monocytopenia", "probability": 0.72548828125}, {"start": 4298.62, "end": 4298.72, "word": " ..", "probability": 0.307373046875}, {"start": 4298.72, "end": 4300.3, "word": " monocytopenia", "probability": 0.8767578125}, {"start": 4300.3, "end": 4301.04, "word": " طبعا", "probability": 0.9090576171875}, {"start": 4301.04, "end": 4302.26, "word": " طيب", "probability": 0.7779134114583334}, {"start": 4302.26, "end": 4303.68, "word": " ال", "probability": 0.88916015625}, {"start": 4303.68, "end": 4303.8, "word": " ..", "probability": 0.1640625}, {"start": 4303.8, "end": 4304.52, "word": " ال", "probability": 0.70703125}, {"start": 4304.52, "end": 4304.88, "word": " neutral", "probability": 0.430419921875}, {"start": 4304.88, "end": 4305.16, "word": " في", "probability": 0.361572265625}, {"start": 4305.16, "end": 4306.34, "word": " الزيادتها", "probability": 0.9049479166666666}, {"start": 4306.34, "end": 4311.66, "word": " neutrophilia", "probability": 0.9122721354166666}, {"start": 4311.66, "end": 4312.26, "word": " ..", "probability": 0.6552734375}, {"start": 4312.26, "end": 4312.84, "word": " طبعا", "probability": 0.825439453125}, {"start": 4312.84, "end": 4314.08, "word": " neutrophilia", "probability": 0.9064127604166666}, {"start": 4314.08, "end": 4315.6, "word": " و", "probability": 0.8642578125}, {"start": 4315.6, "end": 4316.66, "word": " نقصانها", "probability": 0.9396484375}], "temperature": 1.0}, {"id": 189, "seek": 434635, "start": 4324.91, "end": 4346.35, "text": "neutropenia نفس الأشي ال azenophil زيادتها azenophilia ونقصانها azenopenia, bezophilia, bezocytopenia طبعا فبالتالي لازم هذه الشغلات أنتوا تكونوا بتعرفوها أو المصطلحات تكونوا بتعرفوها و تكونوا بتعرفوا إيش فكرة ال difference يعني طبعا لكن أنه", "tokens": [716, 325, 1513, 268, 654, 8717, 36178, 16247, 8592, 1829, 2423, 257, 2904, 5317, 388, 30767, 1829, 18513, 2655, 11296, 257, 2904, 5317, 24169, 4032, 1863, 4587, 9381, 7649, 11296, 257, 2904, 15752, 654, 11, 10782, 5317, 24169, 11, 10782, 31078, 19337, 268, 654, 23032, 3555, 3615, 995, 6156, 3555, 6027, 2655, 6027, 1829, 5296, 31377, 2304, 29538, 25124, 17082, 1211, 9307, 14739, 2655, 14407, 6055, 30544, 14407, 39894, 3615, 28480, 2407, 11296, 34051, 9673, 9381, 9566, 1211, 5016, 9307, 6055, 30544, 14407, 39894, 3615, 28480, 2407, 11296, 4032, 6055, 30544, 14407, 39894, 3615, 28480, 14407, 11933, 1829, 8592, 6156, 4117, 25720, 2423, 2649, 37495, 22653, 23032, 3555, 3615, 995, 44381, 14739, 3224], "avg_logprob": -0.1987390386169417, "compression_ratio": 1.759433962264151, "no_speech_prob": 2.8014183044433594e-06, "words": [{"start": 4324.91, "end": 4325.71, "word": "neutropenia", "probability": 0.864453125}, {"start": 4325.71, "end": 4326.51, "word": " نفس", "probability": 0.71240234375}, {"start": 4326.51, "end": 4326.89, "word": " الأشي", "probability": 0.6993815104166666}, {"start": 4326.89, "end": 4326.99, "word": " ال", "probability": 0.64404296875}, {"start": 4326.99, "end": 4327.65, "word": " azenophil", "probability": 0.41864013671875}, {"start": 4327.65, "end": 4328.25, "word": " زيادتها", "probability": 0.9865234375}, {"start": 4328.25, "end": 4329.27, "word": " azenophilia", "probability": 0.88671875}, {"start": 4329.27, "end": 4332.01, "word": " ونقصانها", "probability": 0.8978678385416666}, {"start": 4332.01, "end": 4333.23, "word": " azenopenia,", "probability": 0.932861328125}, {"start": 4333.59, "end": 4334.37, "word": " bezophilia,", "probability": 0.8434244791666666}, {"start": 4334.77, "end": 4336.37, "word": " bezocytopenia", "probability": 0.653662109375}, {"start": 4336.37, "end": 4336.89, "word": " طبعا", "probability": 0.7894287109375}, {"start": 4336.89, "end": 4338.59, "word": " فبالتالي", "probability": 0.9326171875}, {"start": 4338.59, "end": 4339.65, "word": " لازم", "probability": 0.9954427083333334}, {"start": 4339.65, "end": 4339.85, "word": " هذه", "probability": 0.83544921875}, {"start": 4339.85, "end": 4340.25, "word": " الشغلات", "probability": 0.9765625}, {"start": 4340.25, "end": 4340.55, "word": " أنتوا", "probability": 0.68896484375}, {"start": 4340.55, "end": 4340.73, "word": " تكونوا", "probability": 0.9095052083333334}, {"start": 4340.73, "end": 4341.35, "word": " بتعرفوها", "probability": 0.97431640625}, {"start": 4341.35, "end": 4341.53, "word": " أو", "probability": 0.626953125}, {"start": 4341.53, "end": 4341.99, "word": " المصطلحات", "probability": 0.9798990885416666}, {"start": 4341.99, "end": 4342.25, "word": " تكونوا", "probability": 0.8702799479166666}, {"start": 4342.25, "end": 4342.79, "word": " بتعرفوها", "probability": 0.98759765625}, {"start": 4342.79, "end": 4342.89, "word": " و", "probability": 0.79833984375}, {"start": 4342.89, "end": 4343.13, "word": " تكونوا", "probability": 0.8614908854166666}, {"start": 4343.13, "end": 4343.53, "word": " بتعرفوا", "probability": 0.9827880859375}, {"start": 4343.53, "end": 4343.69, "word": " إيش", "probability": 0.830078125}, {"start": 4343.69, "end": 4343.95, "word": " فكرة", "probability": 0.9920247395833334}, {"start": 4343.95, "end": 4344.17, "word": " ال", "probability": 0.9248046875}, {"start": 4344.17, "end": 4344.57, "word": " difference", "probability": 0.8662109375}, {"start": 4344.57, "end": 4344.87, "word": " يعني", "probability": 0.88525390625}, {"start": 4344.87, "end": 4345.57, "word": " طبعا", "probability": 0.932861328125}, {"start": 4345.57, "end": 4345.93, "word": " لكن", "probability": 0.6494140625}, {"start": 4345.93, "end": 4346.35, "word": " أنه", "probability": 0.672119140625}], "temperature": 1.0}, {"id": 190, "seek": 436739, "start": 4346.61, "end": 4367.39, "text": "فعليا تفاصيل ال differential إيش مطلوب منكم أو كيف أنه تعملوا هذا تاخدوا في ال hematology تمام؟ بهيك بنكون وصلنا لنهاية محاضرتنا لليوم ان شاء الله تكون واضحة وسهلة بالنسبة لإلكم ويعطيكم ألف عافية", "tokens": [5172, 3615, 20292, 995, 6055, 5172, 33546, 26895, 2423, 15756, 11933, 1829, 8592, 3714, 9566, 1211, 37746, 9154, 24793, 34051, 9122, 33911, 14739, 3224, 6055, 25957, 1211, 14407, 23758, 6055, 47283, 3215, 14407, 8978, 2423, 8636, 267, 1793, 46811, 10943, 22807, 39627, 1829, 4117, 44945, 30544, 4032, 36520, 8315, 5296, 1863, 11296, 10632, 3714, 5016, 46958, 43500, 8315, 5296, 20292, 20498, 16472, 13412, 16606, 21984, 6055, 30544, 4032, 46958, 5016, 3660, 46952, 3224, 37977, 20666, 1863, 35457, 3660, 5296, 28814, 23275, 2304, 4032, 1829, 3615, 9566, 1829, 24793, 5551, 46538, 6225, 31845, 10632], "avg_logprob": -0.15242686376292655, "compression_ratio": 1.543778801843318, "no_speech_prob": 2.4437904357910156e-06, "words": [{"start": 4346.61, "end": 4347.11, "word": "فعليا", "probability": 0.80609130859375}, {"start": 4347.11, "end": 4347.69, "word": " تفاصيل", "probability": 0.9486083984375}, {"start": 4347.69, "end": 4348.55, "word": " ال", "probability": 0.97021484375}, {"start": 4348.55, "end": 4349.01, "word": " differential", "probability": 0.90185546875}, {"start": 4349.01, "end": 4349.33, "word": " إيش", "probability": 0.775146484375}, {"start": 4349.33, "end": 4349.65, "word": " مطلوب", "probability": 0.937744140625}, {"start": 4349.65, "end": 4350.03, "word": " منكم", "probability": 0.973388671875}, {"start": 4350.03, "end": 4350.75, "word": " أو", "probability": 0.91455078125}, {"start": 4350.75, "end": 4351.01, "word": " كيف", "probability": 0.9619140625}, {"start": 4351.01, "end": 4351.33, "word": " أنه", "probability": 0.750732421875}, {"start": 4351.33, "end": 4352.03, "word": " تعملوا", "probability": 0.783447265625}, {"start": 4352.03, "end": 4352.89, "word": " هذا", "probability": 0.72314453125}, {"start": 4352.89, "end": 4353.23, "word": " تاخدوا", "probability": 0.8226318359375}, {"start": 4353.23, "end": 4353.31, "word": " في", "probability": 0.92578125}, {"start": 4353.31, "end": 4353.41, "word": " ال", "probability": 0.96923828125}, {"start": 4353.41, "end": 4355.01, "word": " hematology", "probability": 0.82568359375}, {"start": 4355.01, "end": 4357.65, "word": " تمام؟", "probability": 0.7721354166666666}, {"start": 4357.65, "end": 4358.01, "word": " بهيك", "probability": 0.75439453125}, {"start": 4358.01, "end": 4358.67, "word": " بنكون", "probability": 0.94580078125}, {"start": 4358.67, "end": 4359.37, "word": " وصلنا", "probability": 0.9847005208333334}, {"start": 4359.37, "end": 4360.43, "word": " لنهاية", "probability": 0.926025390625}, {"start": 4360.43, "end": 4362.41, "word": " محاضرتنا", "probability": 0.93876953125}, {"start": 4362.41, "end": 4363.01, "word": " لليوم", "probability": 0.9352213541666666}, {"start": 4363.01, "end": 4363.87, "word": " ان", "probability": 0.4951171875}, {"start": 4363.87, "end": 4364.01, "word": " شاء", "probability": 0.981689453125}, {"start": 4364.01, "end": 4364.15, "word": " الله", "probability": 0.9609375}, {"start": 4364.15, "end": 4364.49, "word": " تكون", "probability": 0.959716796875}, {"start": 4364.49, "end": 4364.87, "word": " واضحة", "probability": 0.958984375}, {"start": 4364.87, "end": 4365.21, "word": " وسهلة", "probability": 0.8753255208333334}, {"start": 4365.21, "end": 4365.59, "word": " بالنسبة", "probability": 0.9168701171875}, {"start": 4365.59, "end": 4366.03, "word": " لإلكم", "probability": 0.7938232421875}, {"start": 4366.03, "end": 4366.57, "word": " ويعطيكم", "probability": 0.866455078125}, {"start": 4366.57, "end": 4366.93, "word": " ألف", "probability": 0.7939453125}, {"start": 4366.93, "end": 4367.39, "word": " عافية", "probability": 0.9677734375}], "temperature": 1.0}], "language": "ar", "language_probability": 1.0, "duration": 4370.994, "duration_after_vad": 4272.1312499999985}
\ No newline at end of file
diff --git a/PL9fwy3NUQKwY9jl8xw-bWkVZkWcGGyspd/ELhdSBMOyuo_raw.srt b/PL9fwy3NUQKwY9jl8xw-bWkVZkWcGGyspd/ELhdSBMOyuo_raw.srt
new file mode 100644
index 0000000000000000000000000000000000000000..e8677e61d6ccfd2e22a0c7653adea71ac711ab14
--- /dev/null
+++ b/PL9fwy3NUQKwY9jl8xw-bWkVZkWcGGyspd/ELhdSBMOyuo_raw.srt
@@ -0,0 +1,3700 @@
+1
+00:00:11,190 --> 00:00:14,530
+السلام عليكم ورحمة الله وبركاته طالبات العزيزات
+
+2
+00:00:14,530 --> 00:00:18,470
+اليوم ان شاء الله راح نتحدث عن blood smear
+
+3
+00:00:18,470 --> 00:00:22,770
+examination and preparation تمام؟ هو blood smear
+
+4
+00:00:22,770 --> 00:00:27,750
+preparation and examination المفروض اليوم نتعلم شو
+
+5
+00:00:27,750 --> 00:00:31,970
+يعني مسحة الدم؟ كيف أنا بحضر هاي المسحة؟ وكيف ممكن
+
+6
+00:00:31,970 --> 00:00:35,780
+أنا أسبخها و أشوفها تحت ال microscope؟لحتى أستكشف
+
+7
+00:00:35,780 --> 00:00:41,800
+خلايا الدم في البداية ال blood smear هو عبارة عن
+
+8
+00:00:41,800 --> 00:00:46,940
+مسحة أو فرد للدم على الشريحة تمام؟ فرد لعينة الدم
+
+9
+00:00:46,940 --> 00:00:50,940
+على الشريحة بحيث اني انا بإمكاني ان انا اثبت هذه
+
+10
+00:00:50,940 --> 00:00:55,560
+العينة على الشريحة و من ثم اصبغها و بعد هيك اشوف
+
+11
+00:00:55,560 --> 00:01:00,180
+اتعرف على أشكال خلايا الدم ليش طيب انا بدى اتعرف
+
+12
+00:01:00,180 --> 00:01:04,620
+على ال morphology؟of RBCs أو .. أو ال morphology
+
+13
+00:01:04,620 --> 00:01:10,480
+of white BCs لإنه أشكال خلايا الدم سواء كانت white
+
+14
+00:01:10,480 --> 00:01:16,580
+BCs أو RBCs بتكون ذات دلالة أو بتساعدنا في ال
+
+15
+00:01:16,580 --> 00:01:23,210
+diagnosis في تشخيص بعد الأمراض تمام؟مثلا إذا كانت
+
+16
+00:01:23,210 --> 00:01:27,410
+.. إذا كان الشخص عنده anemia فأنا بلاحظ إن ال RBC
+
+17
+00:01:27,410 --> 00:01:31,670
+بتكون فاضية أو مافيش فيها لون أحمر كتير تمام؟ إذا
+
+18
+00:01:31,670 --> 00:01:36,010
+كان مثلا في عندي white bc كتيرة أو ال white bc هذي
+
+19
+00:01:36,010 --> 00:01:39,610
+immature معناها بشك أن الشخص ممكن يكون مصاب
+
+20
+00:01:39,610 --> 00:01:46,410
+بلوكيميا حتى يعني زيادة نوع واحد من الخلايا هذا
+
+21
+00:01:46,410 --> 00:01:53,470
+أيضا بيرتبط ب ..بتشخيص معين إذا أنا لازم أتعرف على
+
+22
+00:01:53,470 --> 00:01:57,810
+أشكال الخلايا هاي و أتعرف ممكن كمان إن أنا أعد أو
+
+23
+00:01:57,810 --> 00:02:03,250
+أعمل differential لإلها لحتى أتمكن من المساعدة في
+
+24
+00:02:03,250 --> 00:02:08,410
+التشخيص تمام ال blood film هذا دايما بيكون مرافق
+
+25
+00:02:08,410 --> 00:02:11,630
+لل CBC يعني إذا أنا عملت CBC أو عملت مثلا ال
+
+26
+00:02:11,630 --> 00:02:17,090
+homoglobin test عملت hematocrit حسبة ال MCV و ال
+
+27
+00:02:17,090 --> 00:02:26,370
+.. ال ..قانون التلاتة مثلا الأشياء هاي فطبعا لازم
+
+28
+00:02:26,370 --> 00:02:31,790
+معها أكون عملت برضه كمان blood film there are
+
+29
+00:02:31,790 --> 00:02:35,290
+three types of blood smear طبعا blood smear أو
+
+30
+00:02:35,290 --> 00:02:39,290
+blood film نفس المعنى اللي هي مسحة الدم في عندي
+
+31
+00:02:39,290 --> 00:02:43,670
+تلت أنواع منهامسحة تجرع على ال cover glass أو اللي
+
+32
+00:02:43,670 --> 00:02:47,650
+هو ال .. ال .. ال cover slip اللي أنتوا كنتوا
+
+33
+00:02:47,650 --> 00:02:51,810
+تستخدموه لحتى تغطوا ال chamber ممكن أنا أفرد عليه
+
+34
+00:02:51,810 --> 00:02:56,730
+الدم فبسميه cover glass سمير ممكن أنا بسم .. أعمل
+
+35
+00:02:56,730 --> 00:03:02,490
+wedge سمير باستخدام شريحتين زجاجيات طبعا فبنسمي
+
+36
+00:03:02,490 --> 00:03:07,770
+هذا ال .. ال .. السمير wedge سمير طبعاأو ال sponge
+
+37
+00:03:07,770 --> 00:03:13,470
+smear باستخدام الأجهزة، تمام؟ يعني هو electronic
+
+38
+00:03:13,470 --> 00:03:18,730
+أو تقني، تقني، تمام؟ آلي، اذا في عندي تلت أنواع من
+
+39
+00:03:18,730 --> 00:03:21,670
+ال blood films أو ال blood smears، احنا اليوم راح
+
+40
+00:03:21,670 --> 00:03:25,850
+نتحدث و انت راح تتعلموا بالمختبر ال wedge smear،
+
+41
+00:03:25,850 --> 00:03:29,930
+تمام؟ اللي هو باستخدام two slides، باستخدام two
+
+42
+00:03:29,930 --> 00:03:34,840
+slidesطبعا في عندي أنواع أخرى من ال blood smears
+
+43
+00:03:34,840 --> 00:03:40,020
+أو ال blood film بيتم تحضيرهم for specific
+
+44
+00:03:40,020 --> 00:03:46,240
+purposes لأغراض خاصة مثلا buffy coat smearيعني أنه
+
+45
+00:03:46,240 --> 00:03:51,340
+أنا أفصل الدم باستخدام ال centrifuge و أخد عينة من
+
+46
+00:03:51,340 --> 00:03:54,680
+منطقة ال buffy code تمام علشان أنا أفرضها على ال
+
+47
+00:03:54,680 --> 00:04:02,640
+slide و أشوف ال white pieces أشكالها و عدادها و
+
+48
+00:04:02,640 --> 00:04:06,700
+هذه تجرى للناس اللي عندهم ال white pieces أقل من
+
+49
+00:04:06,700 --> 00:04:12,300
+ألف تمام يعني عندهم leukocytopenia يعني ليكو سايتس
+
+50
+00:04:12,300 --> 00:04:18,060
+قليلة تمامطيب في نوع آخر من ال blood films أو ال
+
+51
+00:04:18,060 --> 00:04:22,180
+blood smears بنسميه thick blood smears يعني أنه
+
+52
+00:04:22,180 --> 00:04:28,760
+أنا أحضر blood film سميك تمام؟ أحضر blood film
+
+53
+00:04:28,760 --> 00:04:33,580
+سميك وهذا بيستخدم لتشخيص ال blood parasites لتشخيص
+
+54
+00:04:33,580 --> 00:04:38,500
+الطفليات اللي بتعيش في الدم و بتسبب أمراض الدم زي
+
+55
+00:04:38,500 --> 00:04:44,230
+ال malaria مثلاطيب فإذا أنا بحضر لحتى مثلا أشخص
+
+56
+00:04:44,230 --> 00:04:47,570
+malaria لازم يكون في عندي thick blood film إيش
+
+57
+00:04:47,570 --> 00:04:50,330
+يعني thick blood film؟ يعني أنا بستخدم كمية كبيرة
+
+58
+00:04:50,330 --> 00:04:53,950
+من الدم و بفرضها على الشريحة على .. حتى تعطيني ثمك
+
+59
+00:04:53,950 --> 00:04:59,840
+.. تعطي ثماكة للفيلمطيب إيش نوع العينة اللي عندي؟
+
+60
+00:04:59,840 --> 00:05:03,020
+لازم تكون العينة edita anticoagulant ممكن أنا
+
+61
+00:05:03,020 --> 00:05:07,340
+أستخدم أنواع أخرى من ال .. ال anticoagulant ولكن
+
+62
+00:05:07,340 --> 00:05:11,100
+ال most common اللي أكثر شيوعا و أكثر استخداما هو
+
+63
+00:05:11,100 --> 00:05:15,640
+ال edita طبعا لازم تكون العينة خلال ساعتين أو
+
+64
+00:05:15,640 --> 00:05:20,920
+تلاتة من جامعهاانا اجري ال blood film او احضر ال
+
+65
+00:05:20,920 --> 00:05:26,640
+blood film ليش؟ لإنه اذا طولت العينة ممكن يحدث
+
+66
+00:05:26,640 --> 00:05:32,880
+change على ال RBCs morphology مثلا يعني انه تتغير
+
+67
+00:05:32,880 --> 00:05:37,360
+ال RBCs تبدأ خلايا تموت تتكسر ال white BCs ممكن
+
+68
+00:05:37,360 --> 00:05:41,580
+تتغير فبالتالي اذا انا لازم استخدم العينة تكون
+
+69
+00:05:41,580 --> 00:05:45,400
+fresh او خلال ساعتين لتلاتة من ساحبها انا اكون
+
+70
+00:05:45,400 --> 00:05:50,150
+حضرت ال blood filmطيب في عوامل أخرى أيضا تؤثر على
+
+71
+00:05:50,150 --> 00:05:54,090
+شكل ال RBC's ما يتشينج RBC's morphology ممكن
+
+72
+00:05:54,090 --> 00:05:57,150
+تخليها speculated او granulated يعني shrinkage
+
+73
+00:05:57,150 --> 00:06:00,950
+يصيرلها shrinkage تمام؟ ايش اللي ممكن يسببلي
+
+74
+00:06:00,950 --> 00:06:04,510
+الشرنكج؟ اكيد ال excessive amount of anticoagulant
+
+75
+00:06:04,510 --> 00:06:08,710
+يعني اذا انا مثلا ماعبيت دم في طيوب الاديتال عند
+
+76
+00:06:08,710 --> 00:06:12,610
+العلامة معناها الاديتال زيادة هتكون بالنسبة لل
+
+77
+00:06:12,610 --> 00:06:17,890
+bloodفي عندي excessive amount of anticoagulant،
+
+78
+00:06:17,890 --> 00:06:21,130
+إذا ال RBCs راح يصير .. يصيرلها shrinkage، راح ي
+
+79
+00:06:21,130 --> 00:06:25,790
+.. ال shrinkage يعني راح تبرز من الخلية إشي ما
+
+80
+00:06:25,790 --> 00:06:30,750
+يشبه ال .. ال .. الأشواك، تمام؟ إشي ما يشبه
+
+81
+00:06:30,750 --> 00:06:36,450
+الأشواكall the blood longstanding تمام all the
+
+82
+00:06:36,450 --> 00:06:41,470
+blood and longstanding يعني عينة قديمة أو طولت
+
+83
+00:06:41,470 --> 00:06:45,330
+أيضا بتتأثر على ال RBCs morphology و احنا كنا عم
+
+84
+00:06:45,330 --> 00:06:50,790
+نحكي ان ال RBCs او ال blood بشكل عام اذا طولت
+
+85
+00:06:50,790 --> 00:06:55,270
+العينة من ساعتين الى تلاتة او اكتر يعني تجاوزت
+
+86
+00:06:55,270 --> 00:07:01,180
+ساعتين لتلاتة فهذا معناه انهال RBCs رح يبدأ يتغير
+
+87
+00:07:01,180 --> 00:07:05,280
+شكلها ال warm environment .. ال warm environment
+
+88
+00:07:05,280 --> 00:07:10,040
+هادي بتقدي انه انا لما اجي .. يعني جو حار او بيئة
+
+89
+00:07:10,040 --> 00:07:14,880
+دافئة هتقدي الى انه و انا بفرد الدم على الشريحة
+
+90
+00:07:14,880 --> 00:07:21,710
+ممكن يتبخر سائلمن ال plasma تمام هذا راح يأدي إلى
+
+91
+00:07:21,710 --> 00:07:26,790
+جفاف ال blood بسرعة أو اللي هي زيادة تركيزه او
+
+92
+00:07:26,790 --> 00:07:33,370
+جفافه كتير فبالتالي هيأثر على ال .. ال ..ال RBCs
+
+93
+00:07:33,370 --> 00:07:36,470
+morphology ويمكن كنتوا تشوفوا أنتوا أثناء عد ال
+
+94
+00:07:36,470 --> 00:07:41,990
+RBCs على ال chamber لما كنتوا تطولوا بالعد كان يجف
+
+95
+00:07:41,990 --> 00:07:46,550
+ال saline و ال RBCs تلاحظوا أنها هربت إلى المنتصف
+
+96
+00:07:46,550 --> 00:07:50,510
+بحثا عن الرطوبة و تلاحظوا أنه على ال morphology
+
+97
+00:07:50,510 --> 00:07:55,890
+يعني على المربعات كانت ال RBCs تبدو زي كأنه
+
+98
+00:07:55,890 --> 00:07:59,710
+صاريلها shrinkage طبعا يعني صاريلها speculated أو
+
+99
+00:07:59,710 --> 00:08:04,170
+createdهلأ لحتى بس نعرف إيش يعني speculated أو
+
+100
+00:08:04,170 --> 00:08:08,810
+created بدي بس .. حتى أعرفكم إيش يعني ال .. ال ..
+
+101
+00:08:08,810 --> 00:08:16,350
+إنه ال RBCs يصيرلها speculated خليني أحكي
+
+102
+00:08:16,350 --> 00:08:20,330
+Speculated
+
+103
+00:08:20,330 --> 00:08:24,230
+RBCs
+
+104
+00:08:24,230 --> 00:08:31,510
+تمام؟ نشوف الصور إيش يعنيهيك high speculated يعني
+
+105
+00:08:31,510 --> 00:08:35,970
+إنها إلها زي .. زي أشواك، نطوقات بارزة منها، تمام؟
+
+106
+00:08:35,970 --> 00:08:39,590
+ها دي معناه إن ال RBCs صارلها shrinkage و يمكن
+
+107
+00:08:39,590 --> 00:08:42,850
+كنتوا تشوفوا هذا الشكل، كنتوا تشوفوا إن ال RBCs زي
+
+108
+00:08:42,850 --> 00:08:49,270
+إلها هيكأطراف معرجة أو يعني بارز منها بروزات و
+
+109
+00:08:49,270 --> 00:08:52,590
+أثناء ما كانت تجف العينة في عد ال RBCs نفس الاشي
+
+110
+00:08:52,590 --> 00:08:57,870
+أنا ممكن أشوفه في حالة ال blood film لو أنا كنت
+
+111
+00:08:57,870 --> 00:09:00,470
+بستخدم .. لو كان عندي excessive amount of
+
+112
+00:09:00,470 --> 00:09:04,930
+anticoagulant لو العينة قديمة أو حتى لو ال ..
+
+113
+00:09:04,930 --> 00:09:12,060
+البيئة حارة فأدت إلى جفاة في العينة بسرعة تمامطيب،
+
+114
+00:09:12,060 --> 00:09:15,900
+هلأ إيش التجربة اللي أنا بدي أجريها؟ أنا بدي أحط
+
+115
+00:09:15,900 --> 00:09:19,020
+قطرة من الدم، طبعا القطرة هذه لازم يكون حجمها
+
+116
+00:09:19,020 --> 00:09:23,100
+مناسب، ماتكونش كبيرة كتير أو صغيرة كتير على clean
+
+117
+00:09:23,100 --> 00:09:27,920
+glass slide، على glass slide، شريحة زجاجيةتمام؟ و
+
+118
+00:09:27,920 --> 00:09:32,560
+بدي أجيب شريحة أخرى و أفلت فيها هذا القطر عشان هيك
+
+119
+00:09:32,560 --> 00:09:38,100
+أنا بستخدم شريحتين زجاجيتين تمام؟ و طبعا الشريحة
+
+120
+00:09:38,100 --> 00:09:41,460
+اللي أنا بفلت فيها بكون مثبتاها بزاوية من تلاتين
+
+121
+00:09:41,460 --> 00:09:45,870
+لاربعينو بتحكم طبعا هذه الزاوية هي اللي بتتحكملي
+
+122
+00:09:45,870 --> 00:09:49,210
+بال thickness of the smear و هلأ راح نعرف إيش يعني
+
+123
+00:09:49,210 --> 00:09:55,350
+و طبعا بعد ما أنا بفرد الدم على الشريحة بدي أخليه
+
+124
+00:09:55,350 --> 00:10:02,720
+جف خلال الهواء بعدين أنا أحضره للصباغةهذا كل ما
+
+125
+00:10:02,720 --> 00:10:06,180
+يتعلق بالتجربة طبعا بيحكيلي هنا أنه مش لازم أنا
+
+126
+00:10:06,180 --> 00:10:12,040
+أنفخ على الشريحة لحتى تجف، بدي أتركها تجف لحالها
+
+127
+00:10:12,040 --> 00:10:16,700
+لإنه اللي نفخ مع البخار اللي بيطلع ممكن يقدي إلى
+
+128
+00:10:16,700 --> 00:10:20,160
+RBCs artifact يعني أن ال RBCs تتشوه أو يصير في
+
+129
+00:10:20,160 --> 00:10:25,380
+عليها أشياء داخلة مش من .. مش من الخلية ذات نفسها
+
+130
+00:10:26,360 --> 00:10:32,080
+طيب إذا أنا بدي أحكينا place drop of blood بدي أحط
+
+131
+00:10:32,080 --> 00:10:36,500
+قطرة .. قطرة من الدم على الشريحة و باستخدام شريحة
+
+132
+00:10:36,500 --> 00:10:40,880
+أخرى بدي أفرد هذه القطرة طبعا بهذا الشكل بأحطها
+
+133
+00:10:40,880 --> 00:10:45,900
+باتجاه معاكس بخلي قطرة الدم تفرد على طرف الشريحة و
+
+134
+00:10:45,900 --> 00:10:49,980
+ب .. بحركها بهذا الاتجاه بحيث انها .. انه انا كون
+
+135
+00:10:49,980 --> 00:10:54,280
+فردت العينة تمام؟ و هيك بيكون شكل ال .. ال .. ال
+
+136
+00:10:54,280 --> 00:10:59,240
+..مسحة الدم، الدم تم مسحه على الشريحة و هيك بيكون
+
+137
+00:10:59,240 --> 00:11:03,140
+شكله في النهاية بتركه to .. to air dry علشان يجف
+
+138
+00:11:03,140 --> 00:11:09,240
+بالهواءطيب characteristics of good smear إيش هي
+
+139
+00:11:09,240 --> 00:11:14,100
+خواص أو خصائص ال good smear لحتى أحكي أنا هذا
+
+140
+00:11:14,100 --> 00:11:20,460
+الفيلم أو هذه المسحة للدم جيدة أو مواصفاتها جيدة
+
+141
+00:11:20,460 --> 00:11:25,680
+إيش لازم .. إيش الشروط اللي لازم تتحقق فيها؟أول
+
+142
+00:11:25,680 --> 00:11:31,120
+حاجة thick at one end, thinning out to a smooth
+
+143
+00:11:31,120 --> 00:11:34,980
+round feather edge هلأ إيش يعني؟ لازم تكون thick
+
+144
+00:11:34,980 --> 00:11:39,580
+في منطقة و thin في منطقة أخرى، thick في منطقة ال
+
+145
+00:11:39,580 --> 00:11:43,540
+drop عند منطقة ال drop هيكون في أعلى تركيز للدم
+
+146
+00:11:43,540 --> 00:11:49,200
+يعني هتكون thick سميكهيبدأ يخف تركيزه قدام مع أنه
+
+147
+00:11:49,200 --> 00:11:53,360
+كل ما بمسح لقدام شوية معناه أنه هيكون في عندي أقل
+
+148
+00:11:53,360 --> 00:11:58,680
+تركيز فبالتالي هتكون هي سن يعني و لازم تكون
+
+149
+00:11:58,680 --> 00:12:03,060
+أطرافها دائرية و منتظمة ما .. مايتكون مشرشرة
+
+150
+00:12:03,060 --> 00:12:06,700
+أطرافها بهذا الشكل زي ما أنتوا شايفين هي أطرافها
+
+151
+00:12:06,700 --> 00:12:13,870
+دائرية و منتظمةطيب occupy two thirds of the total
+
+152
+00:12:13,870 --> 00:12:18,610
+slide area تحتل تقريبا تلتان الشريحة و أيضا
+
+153
+00:12:19,700 --> 00:12:22,720
+shouldn't touch any edge of the slide مش لازم
+
+154
+00:12:22,720 --> 00:12:28,760
+تلامس أي طرف أو أي حافة من حواف الشريحة تمام؟
+
+155
+00:12:28,760 --> 00:12:34,400
+وكمان should be margin free مايكونش فيها فراغات مع
+
+156
+00:12:34,400 --> 00:12:40,940
+ده منطقة القطرة هاي هنا أنا حطيت القطرة و فردت
+
+157
+00:12:40,940 --> 00:12:46,020
+الشريحة مكان القطرة هيصير في عندي زي الفراغطبعا
+
+158
+00:12:46,020 --> 00:12:50,260
+هذا مسموح فيه لكن لازم المسحة تكون منتظمة ومستمرة
+
+159
+00:12:50,260 --> 00:12:54,800
+ان الدم مفروض بشكل مستمر ومايكون فيه فراغات should
+
+160
+00:12:54,800 --> 00:13:00,460
+be margin free طبعا و as soon as the drop of blood
+
+161
+00:13:00,460 --> 00:13:04,400
+is placed on the glass slide the smear should be
+
+162
+00:13:04,400 --> 00:13:08,060
+made without delay مجرد ما احط قطرة الدم على
+
+163
+00:13:08,060 --> 00:13:12,700
+الشريحة لازم انا اعمل الفردة او ال smearالمسحة
+
+164
+00:13:12,700 --> 00:13:18,600
+بشكل سريع بدون تأخير لإنه أي تأخير راح يقدي إلى
+
+165
+00:13:18,600 --> 00:13:22,220
+أول حاجة abnormal distribution of the white blood
+
+166
+00:13:22,220 --> 00:13:29,380
+cell تمام تاني إشي ممكن حواف القطرة يجفوا وبالتالي
+
+167
+00:13:29,380 --> 00:13:33,280
+يجرحوا الفردة يعني الفردة تصير فيها فراغة تصير
+
+168
+00:13:33,280 --> 00:13:38,490
+فيها تجريحات ما تكونش هيك مستمرة وملساقطبعا
+
+169
+00:13:38,490 --> 00:13:42,990
+فبالتالي بسبب الدم الجاف فبالتالي انا لازم انا
+
+170
+00:13:42,990 --> 00:13:48,090
+اعمل ال .. ال .. الفرد ل .. ل .. ل blood film بشكل
+
+171
+00:13:48,090 --> 00:13:54,070
+سريع طبعا؟ طيب و حكينا ايضا ان هذا بيأثر ..
+
+172
+00:13:54,070 --> 00:13:56,770
+التأخير في الفرد راح يأثر على distribution of
+
+173
+00:13:56,770 --> 00:14:00,930
+white blood cellيعني مثلا ال large white cells
+
+174
+00:14:00,930 --> 00:14:04,530
+accumulating at the thin edges of the smear ممكن
+
+175
+00:14:04,530 --> 00:14:07,950
+يخلي انه ال large white cells يعني الخلايا الكبيرة
+
+176
+00:14:07,950 --> 00:14:13,110
+زي ال .. ال monocyte و ال neutrophil هدولة يروحوا
+
+177
+00:14:13,110 --> 00:14:16,990
+على الأطراف اللي .. اللي هي very thin هنافي الآخر
+
+178
+00:14:16,990 --> 00:14:20,850
+و لما اجى اقعد مثلا انا بعيد في المنطقة اللى بتكون
+
+179
+00:14:20,850 --> 00:14:24,390
+sin فلما اجى اقعد والله ممكن انا احكم ان الشخص هذا
+
+180
+00:14:24,390 --> 00:14:28,890
+مثلا عنده neutrophilia او عنده monocytosis يعني
+
+181
+00:14:28,890 --> 00:14:31,610
+عدد كبير من ال neutrophils او عدد كبير من ال
+
+182
+00:14:31,610 --> 00:14:36,930
+monocytes هذا ممكن يكون خاطر انه بس اتغيرت توزيع
+
+183
+00:14:36,930 --> 00:14:42,970
+الخلايا بسبب اني انا اتأخرت في فرد الشريحة تمام؟
+
+184
+00:14:43,740 --> 00:14:49,000
+طيب the thickness of the spread كيف أنا أتحكم بصمك
+
+185
+00:14:49,000 --> 00:14:55,900
+الفرد أو صمك ال .. ال blood smear فعليا أنا بتحكم
+
+186
+00:14:55,900 --> 00:14:59,700
+فيه من خلال الزاوية حكينا أنا ب .. بستعمل زاوية من
+
+187
+00:14:59,700 --> 00:15:07,660
+تلاتين لاربعين تمام؟ لكن لو أنا شخص أجاني و عنده
+
+188
+00:15:07,660 --> 00:15:12,300
+ال hematocrite عاليتمام؟ ال hemotocrit عالي يعني
+
+189
+00:15:12,300 --> 00:15:19,280
+عنده RBC's يعني كتيرة تمام؟ فبالتالي هذا الشخص أنا
+
+190
+00:15:19,280 --> 00:15:25,360
+لازم أعمله فيلم طويل تمام؟ أفرد الدم على أكبر
+
+191
+00:15:25,360 --> 00:15:29,780
+مساحة ممكنة لازم ال .. ال .. الدم يكون .. ال .. ال
+
+192
+00:15:29,780 --> 00:15:34,490
+.. السمير يكون .. الفيلم يكون طويل و يكون سنفهذا
+
+193
+00:15:34,490 --> 00:15:38,770
+إيش بدي أعمل مع هذه الحالة؟ بدي أقلل الزاوية،
+
+194
+00:15:38,770 --> 00:15:43,450
+تمام؟ بقلل الزاوية فالدم بيفرض معايا كمية أكبر و
+
+195
+00:15:43,450 --> 00:15:47,650
+هيك بيطلع معايا thin طيب لو كان في عندي لوح
+
+196
+00:15:47,650 --> 00:15:54,520
+ماتكرتني يعني الخلايا الدم تركيزها قليلتمام؟ هذا
+
+197
+00:15:54,520 --> 00:15:58,560
+معناه انه انا لازم يكون في عندي ثمك لل .. لل blood
+
+198
+00:15:58,560 --> 00:16:03,140
+film علشان الخلايا ماتكونش هيك بعيدة عن بعض و
+
+199
+00:16:03,140 --> 00:16:08,380
+متفرقة فلازم انا اعلي الزاوية تمام؟ اكبر الزاوية
+
+200
+00:16:08,380 --> 00:16:12,620
+بحيث انه هاي الزاوية اللي انا كبرتها تفردلي الدم
+
+201
+00:16:12,620 --> 00:16:18,660
+على مساحة اقل تمام؟ فبالتالي يكون هو thickماشي،
+
+202
+00:16:18,660 --> 00:16:24,000
+فإذا الزاوية بتتحكملي بطول وسمك ال .. ال .. الفيلم
+
+203
+00:16:24,000 --> 00:16:29,240
+كل ما قلت الزاوية ال .. الفيلم هيكون طويل و سن و
+
+204
+00:16:29,240 --> 00:16:32,140
+هذا بيظبط مع الناس اللي ال hemotocrit عندهم عالي
+
+205
+00:16:32,140 --> 00:16:38,300
+كل ما زادت الزاوية هيكون ال .. ال .. الفيلم قصير و
+
+206
+00:16:38,300 --> 00:16:40,840
+سميك فبالتالي هذا بيظبط مع الناس اللي ال
+
+207
+00:16:40,840 --> 00:16:46,330
+hemotocrit عندهم قليلطيب common causes of boring
+
+208
+00:16:46,330 --> 00:16:51,390
+blood film هلأ إيش ممكن يسببلي أو يعني إيش ممكن
+
+209
+00:16:51,390 --> 00:16:55,890
+يحصل لحتى أحكي عن ال blood film هذا بور سيء أو غير
+
+210
+00:16:55,890 --> 00:17:03,510
+مقبول لقراية أشكال و أعداد و أنواع الخلايا؟هلأ أول
+
+211
+00:17:03,510 --> 00:17:06,990
+حاجة ل drop of blood is too large or too small لو
+
+212
+00:17:06,990 --> 00:17:11,370
+كانت قطرة الدم كتير كبيرة معناها الفيلم هيكون كتير
+
+213
+00:17:11,370 --> 00:17:15,650
+سميك فبالتالي ممكن يعيق عملية التصنيف أنواع
+
+214
+00:17:15,650 --> 00:17:19,750
+الخلايا و دراسة ال morphology تبعها، دراسة شكلهاو
+
+215
+00:17:19,750 --> 00:17:24,010
+اذا كان نفس الاشي ال .. ال .. القطر سميكة كتير
+
+216
+00:17:24,010 --> 00:17:28,790
+ممكن تعطيني فيلم صغير و الفيلم هذا او .. او يكون
+
+217
+00:17:28,790 --> 00:17:34,850
+thin فبالتالي ماتعبرش عن وضع الخلايا، تمام؟
+
+218
+00:17:34,850 --> 00:17:38,890
+فبالتالي لازم يكون الحجم مناسب و ال spreader slide
+
+219
+00:17:38,890 --> 00:17:42,270
+pushed across the slide in jerky manner لو احنا
+
+220
+00:17:44,170 --> 00:17:50,030
+فردنا الدم بشكل متقطع تمام فهذا برضه كمان غلط هذا
+
+221
+00:17:50,030 --> 00:17:54,970
+هيطلع معايا ال .. ال .. الدم هيك بشكل .. بهك ..
+
+222
+00:17:54,970 --> 00:17:58,490
+بهذا الشكل لو أنا مثلا كنت بفرد في الدم و ب .. ب
+
+223
+00:17:58,490 --> 00:18:03,770
+.. ب .. يعني بفرد و ب .. بقففمع الفرد المتقطع هذا
+
+224
+00:18:03,770 --> 00:18:07,510
+راح تظهر عندى اكتر من منطقة وما بينهم فراغات و هذا
+
+225
+00:18:07,510 --> 00:18:11,050
+غير مقبول، هذا احنا حكينا انه من شروط ال blood
+
+226
+00:18:11,050 --> 00:18:14,730
+film انه يكون margin free مافيش فيه فراغات، فإذا
+
+227
+00:18:14,730 --> 00:18:20,170
+هذا غلططيب بعدين لازم كمان failure to keep the
+
+228
+00:18:20,170 --> 00:18:23,210
+entire edge of the spreader slide against the
+
+229
+00:18:23,210 --> 00:18:26,990
+slide while making the smear هاي جملة معناها انه
+
+230
+00:18:26,990 --> 00:18:34,210
+انا يعني هو بصراحة و انا بفرض في ال .. في ال blood
+
+231
+00:18:34,210 --> 00:18:39,190
+film لازم انا اكمل فرد لآخر الشريحة يعني ما اتوقف
+
+232
+00:18:39,190 --> 00:18:44,780
+فجأةلحد ما يخلص معايا الدم لحاله بي .. بيعطي الدم
+
+233
+00:18:44,780 --> 00:18:48,340
+.. لو أول ما يخلص الدم لحاله بيعطي الشكل الدائري،
+
+234
+00:18:48,340 --> 00:18:52,680
+الطرف الدائري هذا، تمام؟ طيب لو أنا .. يعني ما
+
+235
+00:18:52,680 --> 00:18:56,360
+استمرت بالحركة لآخر ال slide، توقفت فجأة، إيش رح
+
+236
+00:18:56,360 --> 00:19:01,040
+يصير في عندى؟ رح يصير بهذا الشكل، إنه في عندي ال
+
+237
+00:19:01,040 --> 00:19:04,860
+.. ال .. ال blood film نهايته بدل ما هي دائرية،
+
+238
+00:19:04,860 --> 00:19:09,520
+نهايته إلها خط، يعني مثلا نتوقع .. نتخيل هذا
+
+239
+00:19:09,520 --> 00:19:14,660
+الفيلمهيك و أنا توقفت فجأة نهايته بدل ما هي دائرية
+
+240
+00:19:14,660 --> 00:19:18,960
+هيك خط، تمام؟ فهد معناها انه انا .. ان انا ما
+
+241
+00:19:18,960 --> 00:19:23,020
+استمرت بالحركة على ال entire slide يعني ماكملت
+
+242
+00:19:23,020 --> 00:19:29,070
+الحركة للاخر فهد غلطfailure to keep the spreader
+
+243
+00:19:29,070 --> 00:19:34,610
+slide at 30 degree angle طبعا اذا انا ما .. يعني
+
+244
+00:19:34,610 --> 00:19:38,110
+ماخلت الزاوية تلاتين او من تلاتين لاربعين فهذا
+
+245
+00:19:38,110 --> 00:19:41,650
+معناها انه انا اذا كبرت الزاوية هيكون عندى short و
+
+246
+00:19:41,650 --> 00:19:48,570
+thick او اذا زغرتها فهيكون عندى thin و long و هذا
+
+247
+00:19:48,570 --> 00:19:52,680
+غلططمام؟ انا فعليا بس بكبر الزاوية في حالة ال
+
+248
+00:19:52,680 --> 00:19:56,120
+decreased hematocrit و بظغر الزاوية في حالة ال
+
+249
+00:19:56,120 --> 00:19:59,780
+increased hematocrit شخص normal او ماعندوش المشكلة
+
+250
+00:19:59,780 --> 00:20:03,880
+هاي فبالتالي لازم الزاوية تكون من 30 ل 40 failure
+
+251
+00:20:03,880 --> 00:20:06,900
+to push the spreader slide completely across the
+
+252
+00:20:06,900 --> 00:20:11,030
+slide حكينا اذا انا ما .. اذا ماحطيتالشريحة اللي
+
+253
+00:20:11,030 --> 00:20:17,290
+أنا بفرد فيها ماكملت حركتها لآخر الشريحة اللي أنا
+
+254
+00:20:17,290 --> 00:20:22,750
+بفرد عليها فبالتالي هذا برضه كمان راح يعطيني سمير
+
+255
+00:20:22,750 --> 00:20:27,170
+نهايته مش دائرية irregular spread with ridges and
+
+256
+00:20:27,170 --> 00:20:33,030
+long tail إذا كان في عنديال .. الفرد مش منتظم و
+
+257
+00:20:33,030 --> 00:20:38,770
+إيه له حواف أو إيه له زي أطراف يعني مغير منتظمة
+
+258
+00:20:38,770 --> 00:20:43,750
+هذا معناه إنه الشريحة اللي أنا بفرض فيها هي إما
+
+259
+00:20:43,750 --> 00:20:50,150
+dirty عليها غبرة أو ممكن تكون مكسرة يعني في ..
+
+260
+00:20:50,150 --> 00:20:56,300
+فيها خدوش أو كسور فهذا بقى أيضا كمان ممكن يأثرHole
+
+261
+00:20:56,300 --> 00:21:01,560
+in films إذا حسيت إنه ال blood film فيه خفورات
+
+262
+00:21:01,560 --> 00:21:07,180
+فهذا معناها إن الشريحة عليها fat أو عليها دهون
+
+263
+00:21:07,180 --> 00:21:12,080
+تمام عليها زيت مثلا فهذه لازم أنا أنظفها بالكحول و
+
+264
+00:21:12,080 --> 00:21:16,820
+أستخدمها بعدين أو أنا أستخدم يعني نوعية أفضل في
+
+265
+00:21:16,820 --> 00:21:20,580
+عندي بعض الشرائح هي already أصلا تيجي محفوظة بشمع
+
+266
+00:21:21,050 --> 00:21:24,790
+فيعني عشان ما تجرح بعض هالبعض فبيكون في عليها طبقة
+
+267
+00:21:24,790 --> 00:21:30,530
+رفيعة من الشامع فالمفروض إنه إذا كانت الشريحة أو
+
+268
+00:21:30,530 --> 00:21:34,990
+الشرائح اللي موجودة عندي من هذا النوع فالمفروض إن
+
+269
+00:21:34,990 --> 00:21:38,630
+أنا أنجعها في الكحول ليلة قبل ما أستخدمها يعني
+
+270
+00:21:38,630 --> 00:21:45,210
+overnightتكون محطوطة في .. في jar في كحول قبل
+
+271
+00:21:45,210 --> 00:21:48,910
+الاستخدام علشان الكحول يعمل على إذابة الشمع اللي
+
+272
+00:21:48,910 --> 00:21:52,390
+موجود عليها Cellular degenerative changes إذا كان
+
+273
+00:21:52,390 --> 00:22:00,990
+في عنديتأخير في عملية ال fixation في تثبيت الفيلم
+
+274
+00:22:00,990 --> 00:22:04,110
+على الشريحة أو حتى ال fixative اللي أنا بستخدمه
+
+275
+00:22:04,110 --> 00:22:07,790
+اللي هو ال methanol إذا تم تلويثه بماء أو يعني
+
+276
+00:22:07,790 --> 00:22:11,190
+إيجي عليه ماء أو مطص رطوبة فهذا راح يأثر على
+
+277
+00:22:11,190 --> 00:22:14,210
+الخلايا ممكن يقدي إلى تحلولها تمام؟ يعمللي
+
+278
+00:22:14,210 --> 00:22:18,070
+cellular degenerative changes إذا هذه كلها أسباب
+
+279
+00:22:18,070 --> 00:22:22,760
+ممكن إنها تأثر على جودة ال blood filmهي هذه الو في
+
+280
+00:22:22,760 --> 00:22:26,000
+either edge أو irregular edges يعني أطراف مشارشرة
+
+281
+00:22:26,000 --> 00:22:30,160
+هذا نتيجة إنه إما الشريحة مش نظيفة عليها غبرة أو
+
+282
+00:22:30,160 --> 00:22:33,520
+إن الشريحة اللي أنا بفرض فيها أطرافها مكسرة أو في
+
+283
+00:22:33,520 --> 00:22:37,180
+عليها شقوق هذا ال jerky manner اللي هو الأسلوب
+
+284
+00:22:37,180 --> 00:22:41,580
+المتقطع هذا إنه يعني يُقال إنه كويس بس المفروض إنه
+
+285
+00:22:41,580 --> 00:22:45,300
+يكون أطول شوية هذا too short قليل جدا نتيجة إنه
+
+286
+00:22:45,300 --> 00:22:52,250
+أنا مثلاكبرت الزاوية هذا ارفيع كتير مشكلته أنه
+
+287
+00:22:52,250 --> 00:22:56,150
+يعني لازم يكون هو أعرض من هيك شوية هذه فيها holes
+
+288
+00:22:56,150 --> 00:23:03,470
+نتيجة أن الشريحة عليها شمع أو عليها زيت تمام oil
+
+289
+00:23:03,470 --> 00:23:09,470
+هذه أنه معناها .. هذه الشكل يعني أنه إيدي مالت هيك
+
+290
+00:23:09,470 --> 00:23:13,640
+انتشوحتباتجاه معين و انا بفرد فلأ لازم انا اضل على
+
+291
+00:23:13,640 --> 00:23:18,820
+ال entire هيك ما ا .. ما اميل و اضل باتجاه يعني
+
+292
+00:23:18,820 --> 00:23:22,980
+مستقيم و هذا هيو بيحكيلك failure to keep the
+
+293
+00:23:22,980 --> 00:23:25,720
+entire edge of the spreader slide against the
+
+294
+00:23:25,720 --> 00:23:31,800
+slide while making the smile يعني لازم لحظة
+
+295
+00:23:33,580 --> 00:23:39,080
+لازم حركة ال .. ال .. الشريحة الفاردة اللي بفرض
+
+296
+00:23:39,080 --> 00:23:47,080
+فيها تكون يعني على نفس اتجاه الشريحة، تمام؟ الحركة
+
+297
+00:23:47,080 --> 00:23:49,860
+على نفس الاتجاه، ولكن لما أنا بتبتها، بتبتها
+
+298
+00:23:49,860 --> 00:23:53,140
+بالاتجاه المعاكس، وهذا الكلام كله هورجيكم إياه في
+
+299
+00:23:53,140 --> 00:23:56,460
+العمل، إيش معناه؟ تمام؟ هنا في عندي air drop عشان
+
+300
+00:23:56,460 --> 00:24:01,980
+هيك طلعت في عندي منطقة فاضية في نص القطرة أو في نص
+
+301
+00:24:01,980 --> 00:24:07,970
+الفيلمتمام biological causes of a poor smear يعني
+
+302
+00:24:07,970 --> 00:24:14,430
+أسباب يعني بتأدي إلى أنه السمير يكون سيئ أو رديق
+
+303
+00:24:14,430 --> 00:24:20,110
+ولكن المشكلة في العينة و ليس في تحضير الفيلم أول
+
+304
+00:24:20,110 --> 00:24:27,000
+إشي called agglutininمشكلة إن ال RBCs إذا ما كانت
+
+305
+00:24:27,000 --> 00:24:30,620
+موجودة على درجة حرارة أقل من 37 أو على درجة حرارة
+
+306
+00:24:30,620 --> 00:24:36,620
+الغرفة مثلا ممكن إنه الدم يتجمع أو يتجلط حتى لو
+
+307
+00:24:36,620 --> 00:24:39,300
+أنا حاطة في anticoagulant ممكن يتجلط نتيجة وجود
+
+308
+00:24:39,300 --> 00:24:42,340
+antibody بيجمع ال RBCs مع بعضها البعض و بيقدي إلى
+
+309
+00:24:42,340 --> 00:24:47,640
+aggregation فالحل إنه طبعا هذا ال antibody بيشتغل
+
+310
+00:24:47,640 --> 00:24:51,340
+على درجة حرارة منخفضة يعني درجة حرارة الغرفة فأقل
+
+311
+00:24:51,340 --> 00:24:56,530
+من 25 و تحتطيب فالحل انه انا ادفع العينة احطها على
+
+312
+00:24:56,530 --> 00:25:00,230
+37 علشان هذا ال antibody فك عن الخلايا و يبطل
+
+313
+00:25:00,230 --> 00:25:05,610
+مجمعها و يبطل في عندي agglutination تمام؟ فالفكرة
+
+314
+00:25:05,610 --> 00:25:10,770
+انه انا لو لاحظت انه في شخص عنده هذه المشكلة عنده
+
+315
+00:25:10,770 --> 00:25:14,450
+antibody ممكن يجمع ال RBC يعني عنده حالة called
+
+316
+00:25:14,450 --> 00:25:18,330
+agglutininتمام؟ اللازم إن أنا أعمل incubation لل
+
+317
+00:25:18,330 --> 00:25:22,690
+RBCs على 37 درجة أحطها مثلا في ال water bath أو في
+
+318
+00:25:22,690 --> 00:25:26,190
+ال incubator بعدين أدفع العينة بعدين أحضر منها
+
+319
+00:25:26,190 --> 00:25:33,280
+لسمير لايبينيا إذا الشخص عنده دهونعالية تمام
+
+320
+00:25:33,280 --> 00:25:36,480
+lipemia يعني نسبة الدهون عالية او مستوى الدهون
+
+321
+00:25:36,480 --> 00:25:40,880
+عالي تمام فهذا معناه انه راح يظهر holes حفورات في
+
+322
+00:25:40,880 --> 00:25:44,260
+ال blood film هذه الصراحة المشكلة انا مابقدر يعني
+
+323
+00:25:44,260 --> 00:25:50,570
+احلها لو كانت المشكلة على الفيلمإذا كان ال .. يعني
+
+324
+00:25:50,570 --> 00:25:53,410
+إذا كانت مشكلة الدهون موجودة على الشريحة فأنا ممكن
+
+325
+00:25:53,410 --> 00:25:55,850
+أنظف الشريحة بس إذا كانت الدهون موجودة من العين
+
+326
+00:25:55,850 --> 00:26:01,290
+ذات نفسها أنا ما .. يعني لأ أستطيع إن أنا أصحح هذا
+
+327
+00:26:01,290 --> 00:26:07,250
+الخطأ أو هذه المشكلةRouleau Formation إذا ال RBCs
+
+328
+00:26:07,250 --> 00:26:10,810
+و إنها هي بتعمل stack resembling coins هذا طبعا
+
+329
+00:26:10,810 --> 00:26:13,570
+تعريف ال Rouleau Formation و أظن إنه مار معانا في
+
+330
+00:26:13,570 --> 00:26:18,950
+معمال ال ESR طبعا هذا نتيجة إنه في عندي بروتينات
+
+331
+00:26:18,950 --> 00:26:21,970
+عالية بتجم بت .. بتقلل المسافة ما بين ال RBCs
+
+332
+00:26:21,970 --> 00:26:25,310
+بتقدي إلى تجمعها و ال .. و البروتينات هذه بتكون
+
+333
+00:26:25,310 --> 00:26:28,330
+مرتبطة بحالات مرضية معينة زي ال infection و ال
+
+334
+00:26:28,330 --> 00:26:31,380
+inflammation و ال tissue injuryو الصراحة انا ..
+
+335
+00:26:31,380 --> 00:26:34,820
+يعني اذا الشخص عنده مشكلة مرضية فانا مابقدر أحلها
+
+336
+00:26:34,820 --> 00:26:40,300
+لحتى أمنع انه يتكون ال role and أثناء فرد العينة
+
+337
+00:26:40,300 --> 00:26:47,720
+تمام؟ فهذا طبعا ال .. الاشي مابقدر أحله تقرأ
+
+338
+00:26:47,720 --> 00:26:52,500
+الشريحة و بنعملها examination و هي موجودة لهذه
+
+339
+00:26:52,500 --> 00:26:57,380
+المشكلة عادي يعني بنحاول نعمل .. نعملها skip طيب
+
+340
+00:26:59,340 --> 00:27:03,800
+although this is the easiest and most popular
+
+341
+00:27:03,800 --> 00:27:07,020
+method for producing a blood smear it doesn't
+
+342
+00:27:07,020 --> 00:27:11,680
+produce quality smear as a result of هلأ صح ال ..
+
+343
+00:27:11,680 --> 00:27:15,320
+الوجسمير طريقة الوجسمير اللي هي السمير اللي بيتحدر
+
+344
+00:27:15,320 --> 00:27:20,280
+ب two slides هي أفضل طريقة .. يعني أسهل طريقة هي
+
+345
+00:27:20,280 --> 00:27:25,010
+ال easiest الأسهل و ال most popular الأكثرشيوعا
+
+346
+00:27:25,010 --> 00:27:29,870
+ولكن هي ما بتعطيني يعني سمير جودته عالية جدا
+
+347
+00:27:29,870 --> 00:27:34,270
+مقارنة مثلا بال automated بال .. بال spun تمام؟
+
+348
+00:27:34,270 --> 00:27:41,690
+ليش؟ لأنه أول حاجة ال white PCs أحيانا ما بتتوزعش
+
+349
+00:27:41,690 --> 00:27:44,350
+بشكل منتظم وممكن ال .. و ال RPCs بيصيرلها
+
+350
+00:27:44,350 --> 00:27:49,690
+distortion بتتشوّه شكلها على الحوافأيضا إذا كانت
+
+351
+00:27:49,690 --> 00:27:56,990
+أطراف السمير مشرشرة زي هيك فإنه في عندي .. إنه ال
+
+352
+00:27:56,990 --> 00:27:59,410
+.. ال smaller white pieces زي ال lymphocyte ال
+
+353
+00:27:59,410 --> 00:28:02,850
+smaller white pieces زي ال lymphocyte بتكون موجودة
+
+354
+00:28:02,850 --> 00:28:07,170
+في .. في منتصف هذه الأطراف المشرشرة و ال large
+
+355
+00:28:07,170 --> 00:28:12,610
+lymphocyte اللي هي زي ال monocyte بتكون موجودة على
+
+356
+00:28:12,610 --> 00:28:16,430
+الحواف تمام؟ بتكون موجودة على الحواف يعني لو نيجي
+
+357
+00:28:16,430 --> 00:28:20,280
+هناهنا مثلا على هذه الحافة في المنتصف فيه
+
+358
+00:28:20,280 --> 00:28:24,760
+lymphocyte و على الحواف على الأطراف بيكون موجود ال
+
+359
+00:28:24,760 --> 00:28:28,080
+.. ال monocyte اللي هي ال large lymphocyte .. ال
+
+360
+00:28:28,080 --> 00:28:32,580
+large white piecesSpun Smear reproduce the most
+
+361
+00:28:32,580 --> 00:28:35,460
+uniform distribution of a blood cell يعني ال Spun
+
+362
+00:28:35,460 --> 00:28:39,920
+Smear أو ال automated Smear بيعطيني يعني توزيع
+
+363
+00:28:39,920 --> 00:28:46,620
+أفضل ل .. ل .. ل blood cells طيب حضرت أنا ال ..
+
+364
+00:28:46,620 --> 00:28:52,880
+الشريحة فردت ال blood عملت فيلم و خليته air dry
+
+365
+00:28:52,880 --> 00:28:57,990
+يجف بالهواء هلأ دور اني انا اعملfixation and
+
+366
+00:28:57,990 --> 00:29:02,150
+staining تمام؟ fixation and staining هلأ بنعمل
+
+367
+00:29:02,150 --> 00:29:05,690
+fixation تثبيت لهذه العينة أو لهذا الدم على
+
+368
+00:29:05,690 --> 00:29:10,750
+الشريحة بدي استخدم fixative تمام؟ و هذا ال
+
+369
+00:29:10,750 --> 00:29:14,190
+fixative إيش دوره؟ preserve the morphology of cell
+
+370
+00:29:14,190 --> 00:29:19,650
+بيحافظ على شكل الخلايا تمام؟علشان أتضالها محتفظة
+
+371
+00:29:19,650 --> 00:29:23,290
+بشكلها و لما أجي أنا أدرس ال morphology تبعها يعني
+
+372
+00:29:23,290 --> 00:29:26,630
+أكون شكلها معبر زي كأنها موجودة في ال circulation
+
+373
+00:29:26,630 --> 00:29:33,770
+فبالتالي يعني أشخص صح طيب إذا ال .. ال .. ال films
+
+374
+00:29:33,770 --> 00:29:38,490
+must be fixed لازم .. لازم أثبتها و على طول أول ما
+
+375
+00:29:38,490 --> 00:29:42,210
+اتجفباش بدي أثبتها باستخدام fixative ال fixative
+
+376
+00:29:42,210 --> 00:29:45,550
+اللي أنا بستخدمه ال methyl alcohol اللي هو ال
+
+377
+00:29:45,550 --> 00:29:49,690
+methanol أو ممكن أستخدم ال ethyl alcohol اللي هو
+
+378
+00:29:49,690 --> 00:29:53,290
+ال ethanol طبعا لازم يكون absolute ال methanol
+
+379
+00:29:53,290 --> 00:29:56,670
+طبعا اللي أنا بستخدمه لازم يكون pure و ال ethanol
+
+380
+00:29:56,670 --> 00:30:00,610
+طبعا احنا انتوا ماري عليكم 70% ethanol يعني انا
+
+381
+00:30:00,610 --> 00:30:04,850
+بحضر مثلا باخد 70% من ال ethanol و بحط عليه 30
+
+382
+00:30:04,850 --> 00:30:12,990
+water تمام؟لأ لكن كلمة absolute هذا معناها انه
+
+383
+00:30:12,990 --> 00:30:20,270
+الethyl يعني تقريبا 99.9% pure مافيش عليه اي قطرة
+
+384
+00:30:20,270 --> 00:30:24,250
+ماء تمام؟ و ال methanol لازم ايضا يكون pure مش
+
+385
+00:30:24,250 --> 00:30:29,010
+مخلوط بماء زي ما بنشتريه من الشركة مايكونش عليه
+
+386
+00:30:29,010 --> 00:30:34,730
+ماء او معموله dilution بالماء نهائيا تمام؟ ليش؟
+
+387
+00:30:34,730 --> 00:30:39,920
+لإن الماءبيقدّي إلى تغيرات، degenerative changes،
+
+388
+00:30:39,920 --> 00:30:44,580
+بيقدّي إلى تحلل الخلايا أو تكسّرها أو ممكن يعملي
+
+389
+00:30:44,580 --> 00:30:51,520
+artifact، يعيق عملية الرؤية، فبالتالي لازم يكون ال
+
+390
+00:30:51,520 --> 00:30:55,140
+fixative pure علشان تكون جودته عالية ويثبتلي
+
+391
+00:30:55,140 --> 00:30:58,480
+الخلايا ويعملي preserve لل morphology بشكل جيد
+
+392
+00:31:03,360 --> 00:31:07,400
+طبعا أنا بكون حاطة هذا الكحول في جار في مرتبان
+
+393
+00:31:07,400 --> 00:31:12,300
+زجاجي و هذا المرتبان فيه فرزات لإنه تجلس الشريحة
+
+394
+00:31:12,300 --> 00:31:16,620
+فيه فبالتالي و المرتبان بيكون بيستوعب أكتر من
+
+395
+00:31:16,620 --> 00:31:21,880
+شريحة فبحط الشريحة فيه و بحسب من دقيقتين إلى تلت
+
+396
+00:31:21,880 --> 00:31:26,100
+دقائق و بعدين بطلعها و بخسلها بعد هيك بالماء طبعا
+
+397
+00:31:26,400 --> 00:31:32,240
+لكن قبل لأ ممنوع و الشريحة يلامسها ماء و ممنوع
+
+398
+00:31:32,240 --> 00:31:34,560
+ايضا ان انا انفخ عليها عشان مايجيش بخار الماء
+
+399
+00:31:34,560 --> 00:31:37,840
+عليها و ايضا كمان ممنوع ان انا استخدم fixative
+
+400
+00:31:37,840 --> 00:31:44,110
+سارله تخفيف او يلامسه الماء تمام؟بعد التخفيق ..
+
+401
+00:31:44,110 --> 00:31:47,390
+بعد ال fixation مش مشكلة لو أنا غسلت بالماء طيب
+
+402
+00:31:47,390 --> 00:31:50,710
+هنا بيحكيلي in humid climates it might necessary
+
+403
+00:31:50,710 --> 00:31:54,630
+to replace the methanol 2 to 3 times per day هلأ
+
+404
+00:31:54,630 --> 00:31:58,270
+بيحكيلي انه .. انه في ال .. في الأجواء الرطبة يفضل
+
+405
+00:31:58,270 --> 00:32:01,470
+انه إذا احنا بنشتغل يعني و في blood films كتير
+
+406
+00:32:01,470 --> 00:32:05,270
+بتيجي على المختبر و بدنا نحضرها و نصبغها فبالتالي
+
+407
+00:32:05,270 --> 00:32:07,890
+لازم انه ال methanol يتغير من مرتين لتلاتة في
+
+408
+00:32:07,890 --> 00:32:12,700
+اليوملأنه في هاي الأجواء أو في هاي ال .. ال ..
+
+409
+00:32:12,700 --> 00:32:16,840
+المناخ الرطب ال methanol ممكن يمتص رطوبة و ممكن
+
+410
+00:32:16,840 --> 00:32:21,440
+يصير له dilution أو انه يصير فيه وصله ميه من رطوبة
+
+411
+00:32:21,440 --> 00:32:25,540
+الجو فبالتالي هذا يأثر على عملية ال fixation تمام؟
+
+412
+00:32:26,410 --> 00:32:32,950
+ممكن القديم اللي أنا بدي أستبدله يعني ممكن أستخدمه
+
+413
+00:32:32,950 --> 00:32:39,270
+علشان أنظف فيه الخلايا خصوصا لو كانت الخلايا يعني
+
+414
+00:32:39,270 --> 00:32:45,170
+مثلا عليها الشمع، المادة الشمعية أو عليها زيت أو
+
+415
+00:32:45,170 --> 00:32:49,530
+حتى كمان يعني أنه يعني عليها أي artifact أو
+
+416
+00:32:49,530 --> 00:32:50,430
+contamination
+
+417
+00:32:52,970 --> 00:32:56,070
+it's important to prevent contact with water
+
+418
+00:32:56,070 --> 00:33:01,130
+before fixation is complete طبعا ممنوع هنا ممنوع
+
+419
+00:33:01,130 --> 00:33:04,970
+to prevent contact with water ممنوع الماء يلامس
+
+420
+00:33:04,970 --> 00:33:07,730
+الشريحة قبل ال fixation حكينا بعد ال fixation مش
+
+421
+00:33:07,730 --> 00:33:11,810
+مشكلة لكن قبل ال fixation ممنوع ملامسة الماء ممنوع
+
+422
+00:33:11,810 --> 00:33:14,330
+ال methanol او ال ethanol absolute اللي انا بدى
+
+423
+00:33:14,330 --> 00:33:19,980
+استخدمه يكون عليه ماء تمام؟ليش؟ لإنه the presence
+
+424
+00:33:19,980 --> 00:33:23,380
+of water during methanol fixation produce
+
+425
+00:33:23,380 --> 00:33:26,720
+refractile body artifact water spot in the
+
+426
+00:33:26,720 --> 00:33:31,060
+erythrocyte تمام؟ هذا رح يقديلي إلى artifact إلى
+
+427
+00:33:31,060 --> 00:33:35,090
+أشياء داخلة على ال RBC أزاي كأنها هيك spotنقاط
+
+428
+00:33:35,090 --> 00:33:39,870
+لامعة هذه النقاط اللامعة ممكن إنها تعيق عملية
+
+429
+00:33:39,870 --> 00:33:44,170
+الرؤية ممكن إنها اتشتت الشخص اللي بيقرأ أو بيقيم
+
+430
+00:33:44,170 --> 00:33:48,090
+ال blood film وممكن أيضا إنها تتداخل في ال
+
+431
+00:33:48,090 --> 00:33:52,270
+diagnosis لإن أحيانا أنا بكون بدور على spots معينة
+
+432
+00:33:52,270 --> 00:33:59,600
+داخل ال RBCs علشان أشقص مرض معين أويعني أشخص حالة
+
+433
+00:33:59,600 --> 00:34:05,280
+فبالتالي وجود هاي ال .. ال artifact و يكون سببها
+
+434
+00:34:05,280 --> 00:34:10,240
+الماء ممكن يعطيني diagnosis خاطرthis water spot
+
+435
+00:34:10,240 --> 00:34:16,800
+هاي النقاط أو القطرات من الماء بتبقى موجودة حتى
+
+436
+00:34:16,800 --> 00:34:22,520
+بعد ما أصبغ العينة تمام؟ و ممكن كمان بتكون موجودة
+
+437
+00:34:22,520 --> 00:34:27,480
+ممكن أنها cover items ممكن تغطي أشياء يعني تيجي
+
+438
+00:34:27,480 --> 00:34:33,400
+على خلايا أو تيجي على مثلا طفيل أو أي إشي موجود
+
+439
+00:34:33,400 --> 00:34:37,020
+داخل الخلايا أو موجود في الدم و تغطيه
+
+440
+00:34:40,260 --> 00:34:44,060
+أيضا they are distracting the person ممكن تشتت
+
+441
+00:34:44,060 --> 00:34:48,880
+الشخص لإنها لامعة هيك و .. يعني بتشتت الشخص أو
+
+442
+00:34:48,880 --> 00:34:53,760
+تزرج الشخص اللي بيقيم ال .. ال blood film و in
+
+443
+00:34:53,760 --> 00:34:56,580
+some cases the water spit may .. the water spots
+
+444
+00:34:56,580 --> 00:34:59,340
+may interfere with diagnosis و حكينا إنها ممكن
+
+445
+00:34:59,340 --> 00:35:03,020
+تأثر على ال diagnosisto prevent the alcohol from
+
+446
+00:35:03,020 --> 00:35:07,740
+becoming contaminated by absorbed water طبعا علشان
+
+447
+00:35:07,740 --> 00:35:12,620
+امنع الكحول من انه يمتص ماء او رطوبة من الجو او
+
+448
+00:35:12,620 --> 00:35:16,600
+نصيرله contaminated ب .. بماء لازم انه يكون موجود
+
+449
+00:35:16,600 --> 00:35:20,540
+في bottle with tightly fitting stopper و يكون مغطى
+
+450
+00:35:20,540 --> 00:35:26,090
+بشكل كتير منيح andnot left exposed to the
+
+451
+00:35:26,090 --> 00:35:30,490
+atmosphere و أنا مثلا مابنفع أحطه في الجار و أترك
+
+452
+00:35:30,490 --> 00:35:35,610
+الجار مفتوح، لهيك بيمتص رطوبة و بيأثر على الشغل،
+
+453
+00:35:35,610 --> 00:35:38,270
+تمام؟ و خصوصا في ال humid climates، خصوصا في
+
+454
+00:35:38,270 --> 00:35:43,510
+الأجواء الراطبة أو في الرطوبة العاليةتمام اذا هيك
+
+455
+00:35:43,510 --> 00:35:47,310
+احنا خلصنا ال fixation ثبتنا الخلايا واحتفظنا
+
+456
+00:35:47,310 --> 00:35:51,810
+بشكلها ومنعنا وصولها للماء بعد هيك بعد ال fixation
+
+457
+00:35:51,810 --> 00:35:55,370
+غسلناها مش مشكلة بس قبل لأ ممنوع يلامسها ماء او
+
+458
+00:35:55,370 --> 00:35:59,830
+يكون فيه ال methanol او ال ethanol ملوث بالماءطيب
+
+459
+00:35:59,830 --> 00:36:05,070
+بعد هيك بدي أنا أبدأ أصبغ الشريحة أو أصبغ الفيلم
+
+460
+00:36:05,070 --> 00:36:09,790
+بدي أستخدم Romano Whiskey Stains تمام؟ إيش يعني
+
+461
+00:36:09,790 --> 00:36:12,450
+Romano Whiskey Stains؟ هلأ هال Romano Whiskey
+
+462
+00:36:12,450 --> 00:36:16,910
+Stains هي عبارة عن عائلة من الصبغات تمام؟ هي يعني
+
+463
+00:36:16,910 --> 00:36:20,750
+مش صبغة واحدة هي لأ هي مجموعة هو مصطلح يطلق على
+
+464
+00:36:20,750 --> 00:36:24,790
+مجموعة من الصبغات عائلة من الصبغات تمام؟ هاي
+
+465
+00:36:24,790 --> 00:36:31,840
+الصبغات تستخدم لال ..صبغ ال blood film و بتعطيني
+
+466
+00:36:31,840 --> 00:36:36,400
+نتائج very satisfactory تمام؟ و من أمثلة هذه
+
+467
+00:36:36,400 --> 00:36:40,840
+الصبغات اللي تنتمي لعائلة ال رومانووسكستين ال my
+
+468
+00:36:40,840 --> 00:36:45,180
+.. my grand world gemza تمام؟ و هي مهم .. يعني
+
+469
+00:36:45,180 --> 00:36:49,660
+كتير نيحة لل routine work للشغل ال routine ال
+
+470
+00:36:49,660 --> 00:36:54,080
+gemza stain بتستخدم
+
+471
+00:36:54,080 --> 00:36:57,680
+طبعا تعطيني delicate stain يعني تفاصيل جيدة جدا
+
+472
+00:36:59,740 --> 00:37:04,680
+Lishmans تمام؟unleashments هي عبارة عن the
+
+473
+00:37:04,680 --> 00:37:08,520
+simplest method أفضل أو أبسط طريقة مش أفضل أبسط
+
+474
+00:37:08,520 --> 00:37:12,140
+طريقة يعني في .. في أفضل منها بس هي أبسط طريقة و
+
+475
+00:37:12,140 --> 00:37:16,940
+أسهل طريقة تستخدم لتحضير ال blood film و إحنا
+
+476
+00:37:16,940 --> 00:37:20,700
+بنستخدمها لما بيكون ال blood film مطلوب قراته
+
+477
+00:37:20,700 --> 00:37:23,860
+urgently إيش يعني كلمة urgently هتمور معاكم كتير
+
+478
+00:37:23,860 --> 00:37:29,800
+خلال دراستكم التحليل urgently يعني فحص عاجل أو شو
+
+479
+00:37:29,800 --> 00:37:33,770
+يعنيإيجاك يا عجاتك عينة عاجلان لازم انت تقرا إيها
+
+480
+00:37:33,770 --> 00:37:37,590
+لإنه بيكون الشخص يعني حالته حرجة جدا ما بين الحياة
+
+481
+00:37:37,590 --> 00:37:42,070
+و الموت فانت لازم تشتغليله فحوصاته urgently تمام؟
+
+482
+00:37:42,070 --> 00:37:46,110
+يعني طارق او يعني عاجل هاي معنى كلمة urgently
+
+483
+00:37:46,110 --> 00:37:53,050
+تمام؟ و طبعا ليه .. ليش أنا بستخدمها مع ال urgent
+
+484
+00:37:53,050 --> 00:38:00,360
+cases أو ال urgent samples لإنههي الصبغة منها
+
+485
+00:38:00,360 --> 00:38:04,700
+وفيها fixative وصبغة يعني هي 2 في 1 يعني هي الصبغة
+
+486
+00:38:04,700 --> 00:38:08,760
+نفسها ال powder تم adapt ب methanol فانا مابحتاج
+
+487
+00:38:08,760 --> 00:38:12,180
+لخطوط fixation يعني انا وفرت من 2 ل 3 minute في ال
+
+488
+00:38:12,180 --> 00:38:16,700
+fixation فبحط على طول يعني بعد ما افرض العينة
+
+489
+00:38:16,700 --> 00:38:21,320
+واخليها air dry تمام على طول بصبغها بالشمنالليشمن
+
+490
+00:38:21,320 --> 00:38:24,800
+لإنه هي فيها صبغة وفيها ميثانول هتثبت و تصبغ في
+
+491
+00:38:24,800 --> 00:38:28,420
+نفس الوقت عشان هيك احنا بنحكي عنها simple بسيطة و
+
+492
+00:38:28,420 --> 00:38:31,540
+بتنفع لل urgent cases لحالات الطريقة لإنها مابتاخد
+
+493
+00:38:31,540 --> 00:38:34,640
+وقت خصوصا الشغل اللي بيجي at night مثلا يعني في
+
+494
+00:38:34,640 --> 00:38:38,040
+الليل عينا بعتد في الليل بدنا نقرألها blood film
+
+495
+00:38:38,040 --> 00:38:41,180
+أكيد يعني هي طريقة أو تمر يعني المريض بيكون وضع
+
+496
+00:38:41,180 --> 00:38:45,650
+وحرج عشان هيك بعتوهاfield stains صبغة ال field
+
+497
+00:38:45,650 --> 00:38:53,090
+طبعا هو يعني صبغة سريعة تستخدم لال .. لال thin
+
+498
+00:38:53,090 --> 00:38:56,770
+films أو ال thick films of parasites .. malaria ال
+
+499
+00:38:56,770 --> 00:39:00,950
+parasites، تمام؟ تستخدم لتشخيص التفايليات خصوصا
+
+500
+00:39:00,950 --> 00:39:08,200
+الملارياهلأ كيف تشتغل الرومان ويسكستان؟ يعني سواء
+
+501
+00:39:08,200 --> 00:39:12,700
+كانت جمزة أو رايت أو كانت لشمن أو whatever إيش
+
+502
+00:39:12,700 --> 00:39:17,600
+نوعها، تمام؟ إيه كيف تشتغل؟ هلأ طالما إن هي تنتمي
+
+503
+00:39:17,600 --> 00:39:21,900
+لعائلة الرومان وويسكستان إذا هي عبارة عن صبغة
+
+504
+00:39:21,900 --> 00:39:27,660
+مركبة موجود فيها جزء حامضي وجزء قاعدي، تمام؟ الجزء
+
+505
+00:39:27,660 --> 00:39:32,020
+القاعدي بسميه cationic أو basic dye زي البيثيلين
+
+506
+00:39:32,020 --> 00:39:36,450
+blueو أو أحد مشتقات الأكسدة تبعتها اللي هو ال
+
+507
+00:39:36,450 --> 00:39:41,870
+oxidation product للميثيليني بلو زي ال Azorbi طبعا
+
+508
+00:39:41,870 --> 00:39:46,490
+سواء كانت ميثيليني بلو أو Azorbi طبعا هي صبغة
+
+509
+00:39:46,490 --> 00:39:51,590
+قاعدية cationic طبعا يعني شحنتها موجبة فإذا هي
+
+510
+00:39:51,590 --> 00:39:56,070
+هتصبغ ال anionic أو ال .. ال .. ال acidic أو
+
+511
+00:39:56,070 --> 00:40:01,400
+الأجزاء اللي شحنتها سالبة في الخليةتمام؟ إيش اللي
+
+512
+00:40:01,400 --> 00:40:04,140
+هي الأجزاء الحامضية أو الأجزاء اللي شحنتها سالبة
+
+513
+00:40:04,140 --> 00:40:09,560
+في الخلية؟ مافيش أشهر من الأحماض النووية لل
+
+514
+00:40:09,560 --> 00:40:13,780
+nucleic acid ال DNA و ال RNA فبتصبغهم و بتعطيهم
+
+515
+00:40:13,780 --> 00:40:17,400
+اللون ماباني blue ل grey تمام؟ أحيانا بتعطيهم
+
+516
+00:40:17,400 --> 00:40:22,380
+اللون ال violet تمام؟أيضًا للـ nucleoprotein،
+
+517
+00:40:22,380 --> 00:40:28,480
+بروتينات النواة وحبيبات ال bezophil برضه كمان
+
+518
+00:40:28,480 --> 00:40:33,000
+بتنصبغ فيها و أحيانًا يعني بشكل ضعيف جدًا حبيبات
+
+519
+00:40:33,000 --> 00:40:36,580
+ال neutrophil بتاخد هاي الصبغة طيب، هلأ إيش يعني
+
+520
+00:40:36,580 --> 00:40:39,300
+حبيبات ال bezophil؟ مش إحنا حكينا إنه ال
+
+521
+00:40:39,300 --> 00:40:43,170
+neutrophil و ال bezophil و ال xenophilتمام هدول
+
+522
+00:40:43,170 --> 00:40:47,830
+التلت خلايا بنحكي عنهم granulocyte يعني خلايا
+
+523
+00:40:47,830 --> 00:40:51,870
+محببة الcytoplasm تبعهم فيهم حبيبات؟ اه طيب
+
+524
+00:40:51,870 --> 00:40:56,650
+الbezofilish ليش سميناها أصلا bezofil لإن حبيباتها
+
+525
+00:40:56,650 --> 00:41:04,200
+عبارة عن حبيبات محبةلل basic dye، تمام؟ إذا هي
+
+526
+00:41:04,200 --> 00:41:08,460
+أخدت اسمها من ظهورها على ال blood film، يعني ليش
+
+527
+00:41:08,460 --> 00:41:12,200
+صمينها basophil؟ لأنها تحتوي على حبيبات، phil يعني
+
+528
+00:41:12,200 --> 00:41:15,780
+محبة، تمام؟ جاي من كلمة philic، محبة، لما نقول
+
+529
+00:41:15,780 --> 00:41:21,070
+hydrophilic، محبة للماء، تمام؟ phil محبةو Bezo
+
+530
+00:41:21,070 --> 00:41:26,250
+محبة للقاعدة، اذا حبيباتها تركيبها حامض محبة
+
+531
+00:41:26,250 --> 00:41:31,890
+للصبغة القاعدية اللي هي ال basic dye اللي هي ال
+
+532
+00:41:31,890 --> 00:41:38,140
+methylene blueتمام؟ طيب، وإذاً هل هذا هو المكون
+
+533
+00:41:38,140 --> 00:41:42,460
+الوحيد للصبغة؟ لأ، في مكون آخر بيكون موجود فيها
+
+534
+00:41:42,460 --> 00:41:47,360
+اللي هو الشق الحامضي أو ال anionic أو اللي هو سالب
+
+535
+00:41:47,360 --> 00:41:52,420
+الشحنة زي ال Eocin Y و Eocin B مثال على هذا الشق
+
+536
+00:41:52,420 --> 00:41:57,280
+أو النوع اللي هي صبغة Eocin Y و Eocin Bطبعا هدى
+
+537
+00:41:57,280 --> 00:42:03,940
+الشق أو هدى الجزء او تكوين من الصبغة بيصبغلى ال
+
+538
+00:42:03,940 --> 00:42:10,500
+cationic طبعا هو سالب فهيربط او يرتبط او يصبغ
+
+539
+00:42:10,500 --> 00:42:16,760
+الأجزاء اللى شحنتها موجة بقى زى ال hemoglobin و
+
+540
+00:42:16,760 --> 00:42:19,740
+الazinophilic granule و هيعطيها اللون ما بين ال
+
+541
+00:42:19,740 --> 00:42:25,070
+orange لل red تمام؟طيب ال azinophil لgranule نفس
+
+542
+00:42:25,070 --> 00:42:29,950
+فكرة ال bezophil، هلأ ال azinophil هي خلية
+
+543
+00:42:29,950 --> 00:42:34,290
+granulocyte، في في ال cytoplasm تبعها حبيبات،
+
+544
+00:42:34,290 --> 00:42:39,890
+الحبيبات هادي، phil لمين؟ محب لمين؟ لل-eosin اللي
+
+545
+00:42:39,890 --> 00:42:44,230
+هي لل acidic dye عشان هيكسميناها azinophil granule
+
+546
+00:42:44,230 --> 00:42:49,040
+أو azinophil cellطمام؟ إذا الصبغة بتكون موجود فيها
+
+547
+00:42:49,040 --> 00:42:53,240
+يعني سواء كانت Gemza, Wright, Solishman, Field أي
+
+548
+00:42:53,240 --> 00:42:56,520
+نوع من هذه الصبغات هيكون في methylene blue أو أحد
+
+549
+00:42:56,520 --> 00:42:59,920
+مشتقات الأكسيدات تبعتها زي ال AzorB و هيكون فيها
+
+550
+00:42:59,920 --> 00:43:05,900
+Acy Y و Acy B ال methylene blue هو قاعدي و شحنته
+
+551
+00:43:05,900 --> 00:43:10,060
+موجبة هيصبغ الأشياء السالبة في الشحنة اللي هي
+
+552
+00:43:10,060 --> 00:43:13,480
+الحامضية زي ال DNA و ال RNA و ال nucleoprotein
+
+553
+00:43:14,420 --> 00:43:20,660
+حبيبات ال .. ال .. ال .. بيزوفل تمام؟ طيب أما ال
+
+554
+00:43:20,660 --> 00:43:24,860
+.. الجزء الحامدى اللى هو سالب الشحنة ال anionic زى
+
+555
+00:43:24,860 --> 00:43:30,560
+ال EUCY و EUCB هيرتبط أو يصبغ بال cationic site او
+
+556
+00:43:30,560 --> 00:43:34,320
+اللى هو ايه يعني الأجزاء القاعدية الموجبة الشحنة
+
+557
+00:43:34,320 --> 00:43:37,830
+في الخلية ويعطيها اللون ال orange لل redزي الـ
+
+558
+00:43:37,830 --> 00:43:41,010
+hemoglobin اللى موجود فى ال RBCs هو قاعر يرتبط بال
+
+559
+00:43:41,010 --> 00:43:44,870
+.. ينصبغ بالإيسين و أيضا الازينوفيل ال granule
+
+560
+00:43:44,870 --> 00:43:49,310
+حبيبات الازينوفيل طيب حبيبات للنيوتروفيل من اسمها
+
+561
+00:43:49,310 --> 00:43:53,550
+neutral متعادلة و في المحبة يعنى بتاعها بتنصبغ
+
+562
+00:43:53,550 --> 00:43:56,830
+شوية بال methylene blue و شوية بالازين عشان هيك
+
+563
+00:43:56,830 --> 00:44:00,130
+بتكون لونها دايما حبيبات للنيوتروفيل بيكون لونها
+
+564
+00:44:00,130 --> 00:44:04,630
+pink ماخدة ال .. ده ..يعني خليط من اللون ال blue
+
+565
+00:44:04,630 --> 00:44:08,950
+مع اللون ال orange ل red هذا فبتعطيني اللون ال
+
+566
+00:44:08,950 --> 00:44:13,510
+pink طبعا لازم انا استخدم buffer phosphate buffer
+
+567
+00:44:13,510 --> 00:44:18,050
+اضيفه على الصبغة علشان يؤدي الى تأيينها الشق
+
+568
+00:44:18,050 --> 00:44:22,350
+القاعدى تبعها ياخد الشحنة الموجبة فيصبغل الأجزاء
+
+569
+00:44:22,350 --> 00:44:29,230
+السالبة و الجزء الحامضى ياخد الشحنة السالبة فيصبغل
+
+570
+00:44:29,230 --> 00:44:36,170
+الأجزاء الموجبة في الخليةطبعا هانا ميعطيني طريقة
+
+571
+00:44:36,170 --> 00:44:40,330
+صباغة بالليشمانستان طبعا حاكي ان هين بحضر الاسمير
+
+572
+00:44:40,330 --> 00:44:45,330
+بخليه air-dry على طول بعد ال air-dry بحط الستان
+
+573
+00:44:45,330 --> 00:44:50,150
+ليش؟ لإن الستان هي منها fixative ومنها صبغة في نفس
+
+574
+00:44:50,150 --> 00:44:56,210
+الوقتو بصبغها بعد هيك بخليها تجف و طبعا بحط الصبغة
+
+575
+00:44:56,210 --> 00:44:59,250
+بضيف ال buffer ال buffer بيخلي الصبغة تتأين و تصبغ
+
+576
+00:44:59,250 --> 00:45:03,810
+بتركها من عشر الى خمستاشر دقيقة بغسلها بخليها تجف
+
+577
+00:45:03,810 --> 00:45:08,770
+بعدين بشوفها على ال microscope تمام؟ فإذا احنا
+
+578
+00:45:08,770 --> 00:45:12,410
+هناخد طريقة تانية لإنه هنستخدم الجمزة و هشرحلكم
+
+579
+00:45:12,410 --> 00:45:18,800
+الطريقة بالمختبرطبعا طيب إيش الألوان اللي هتاخدها
+
+580
+00:45:18,800 --> 00:45:22,640
+أجزاء الخلايا؟ طبعا إحنا حكينا أنوية الخلايا موجود
+
+581
+00:45:22,640 --> 00:45:28,560
+فيها DNA و RNA و Nuclear Protein فهتاخد الصبغة
+
+582
+00:45:28,560 --> 00:45:32,320
+القاعدية اللي هي ال methylene blue أو ال azorbia
+
+583
+00:45:32,320 --> 00:45:36,380
+فهتكون مخدة اللون ال purple حكيتلكم هي إما violet
+
+584
+00:45:36,380 --> 00:45:40,470
+أو ال blue فهتكون مخدة اللون ال purpleالـ
+
+585
+00:45:40,470 --> 00:45:44,010
+cytoplasm الـ cytoplasmal erythrocyte فيه
+
+586
+00:45:44,010 --> 00:45:47,110
+هموجلوبيين خلايا الدم الحمرا الـ cytoplasm تبعها
+
+587
+00:45:47,110 --> 00:45:52,110
+مليان هموجلوبيين فهتاخد ال Eucine فهي ينصبغ بال
+
+588
+00:45:52,110 --> 00:45:58,590
+Eucine وتاخد اللون ال Red تمام ال neutrophil ال
+
+589
+00:45:58,590 --> 00:46:03,230
+cytoplasm تبعها موجود في حبيبات و الحبيبات هذه
+
+590
+00:46:03,230 --> 00:46:07,550
+متعادلة فهتاخد ال blue بقدر ما يعني ال methylene
+
+591
+00:46:07,550 --> 00:46:15,000
+blueبقدر ما هتنسبق بال أيوسين Y أو ال .. ال أيوسين
+
+592
+00:46:15,000 --> 00:46:19,860
+دي فبالتالي هيكون لونها ما بين ال orange ل ال pink
+
+593
+00:46:19,860 --> 00:46:23,800
+طبعا ال lymphocytes ال cytoplasm تبعها هيكون blue
+
+594
+00:46:23,800 --> 00:46:28,440
+يعني انسبق بال مثيلين blue تمام ال monocyte هيكون
+
+595
+00:46:28,440 --> 00:46:31,660
+برضه كمان ال cytoplasm تبعها blue و ال mesophil
+
+596
+00:46:31,660 --> 00:46:35,160
+هيكون ال cytoplasm تبعها blueالحبيبات، الحبيبات لل
+
+597
+00:46:35,160 --> 00:46:38,480
+neutral fill حكينا هيكون لونها fine purple أو يعني
+
+598
+00:46:38,480 --> 00:46:43,140
+موف فاتح أو pink، حكينا ليش؟ وال isenophile
+
+599
+00:46:43,140 --> 00:46:47,320
+حبيباتها هتكون مخدة صبغة ال eosin اللي هي red ل
+
+600
+00:46:47,320 --> 00:46:52,060
+orange، ال basophil مخدة صبغة ال basic اللي هي لون
+
+601
+00:46:52,060 --> 00:46:58,360
+ال .. ال ..ال .. ال methylene blue هتكون مخدة
+
+602
+00:46:58,360 --> 00:47:01,680
+اللون اللي ال blue او ال purple او من كتر ما هي
+
+603
+00:47:01,680 --> 00:47:05,320
+bezofil هتكون مخدة ال purple كتير لدرجة انها صارت
+
+604
+00:47:05,320 --> 00:47:11,060
+black .. black تمام؟ ال monocyte هيكون اللي طبعا
+
+605
+00:47:11,060 --> 00:47:14,920
+فيها granules و ما تاخد fine reddish او azerophil
+
+606
+00:47:14,920 --> 00:47:18,950
+طب .. طب لحظةاحنا مش نصنفنا انه ال monocyte و ال
+
+607
+00:47:18,950 --> 00:47:22,370
+lymphocyte هي عبارة عن اي granulocyte يعني مافيش
+
+608
+00:47:22,370 --> 00:47:26,690
+فيها حبيبات شوفوا هو لما صنفوهم حكوا انه ال white
+
+609
+00:47:26,690 --> 00:47:31,010
+species يعني نوعين granulocyte اللي هم ال
+
+610
+00:47:31,010 --> 00:47:34,420
+neutrophil و الesenophil و الbesophilو ال a
+
+611
+00:47:34,420 --> 00:47:37,260
+-granulocyte اللي هما ال monocyte و ال lymphocyte
+
+612
+00:47:37,260 --> 00:47:41,200
+طيب و بعد التصنيف هذا اكتشفوا انه ال monocyte فيها
+
+613
+00:47:41,200 --> 00:47:45,620
+granule ناعمة جدا بنسميها azorophilic granule و
+
+614
+00:47:45,620 --> 00:47:49,220
+هذه بتاخد اللون ال reddish او بتاخد الصبغة اللي هي
+
+615
+00:47:49,220 --> 00:47:55,490
+الصبغة ال a و cطبعا و كيف ال monocyte طبعا بتتحول
+
+616
+00:47:55,490 --> 00:48:00,350
+ل macrophage و بتلتهم و .. و ال .. ال microbes و
+
+617
+00:48:00,350 --> 00:48:04,690
+بتقتلها لازم يكون في عندها حبيبات لإنه يعني العدة
+
+618
+00:48:04,690 --> 00:48:08,150
+و العتاد و أسلحة القتل بتكون مخزنة في الحبيبات هذه
+
+619
+00:48:08,150 --> 00:48:11,630
+ال platelet بيكون فيها حبيبات و مخد اللون ال
+
+620
+00:48:11,630 --> 00:48:17,100
+purpleتمام؟ هاي هنا الخلايا طبعا الخلايا لل
+
+621
+00:48:17,100 --> 00:48:20,800
+neutral fill و الazino fill و ال basophil النواة
+
+622
+00:48:20,800 --> 00:48:25,140
+تبعتهم بتكون segmented تمام؟ بتكون مقسمة و بي ..
+
+623
+00:48:25,140 --> 00:48:31,960
+بيربط ما بينها هيك زي loop تمام؟ يعني ..زي هيك
+
+624
+00:48:31,960 --> 00:48:38,240
+اختناق طبعا طيب جبل تنضج الخلية و بتتقسم النواة
+
+625
+00:48:38,240 --> 00:48:43,780
+بتكون النواة على شكل C-shape زي هيك بهذا الشكل و
+
+626
+00:48:43,780 --> 00:48:48,580
+مش مقسمة فبنسميها band سل طبعا ال neutral فيل إلها
+
+627
+00:48:48,580 --> 00:48:52,660
+band الisinofil إلها band و الbesophil إلها band
+
+628
+00:48:52,750 --> 00:48:56,970
+تمام؟ يعني لازم الخلية أثناء .. قبل ندوجها .. احنا
+
+629
+00:48:56,970 --> 00:48:59,790
+حكينا أنه الـ neutrophil والازينوفل والبيزوفل
+
+630
+00:48:59,790 --> 00:49:06,870
+النواة تبعتها بتكون مقسمة وفي مابينها هيك زي .. زي
+
+631
+00:49:06,870 --> 00:49:12,170
+loop بيربط .. بيربط مابين القطع هذه المقسمةطب جابل
+
+632
+00:49:12,170 --> 00:49:17,630
+ما تتجسم كيف كانت النواة كانت band طبعا لما ندجت
+
+633
+00:49:17,630 --> 00:49:24,410
+الخلية و طلعت من البون مارو على الدم فصارت
+
+634
+00:49:24,410 --> 00:49:30,090
+segmented طبعا فإذا في عندي band neutrophil, band
+
+635
+00:49:30,090 --> 00:49:34,090
+eosinophil, band besophil وفي عندي segmented
+
+636
+00:49:34,090 --> 00:49:37,350
+neutrophil segmented eosinophil, segmented
+
+637
+00:49:37,350 --> 00:49:42,770
+besophil طبعاطيب، هلأ نشوف الـ neutrophil العدد
+
+638
+00:49:42,770 --> 00:49:47,630
+الـ segments للنواء بيتروح ما بين 2 ل 5 طبعا، في
+
+639
+00:49:47,630 --> 00:49:54,470
+عندي من 2 ل 5 loops طبعا، و الحبيبات طبعتها لونها
+
+640
+00:49:54,470 --> 00:49:59,690
+pink و زي ما انتوا شايفين هي خلية كبيرة و ال .. ال
+
+641
+00:49:59,690 --> 00:50:03,650
+cytoplasm طبعها بيكون هيك لونه على pink برضه جاي
+
+642
+00:50:03,650 --> 00:50:06,750
+طيب، ال azinophil .. ال azinophil زي ما انتوا
+
+643
+00:50:06,750 --> 00:50:12,900
+شايفينبتكون النواة غالبا من اتنين لاربعة عدد ال
+
+644
+00:50:12,900 --> 00:50:15,620
+loops تبعتها و ال most common انه يكونوا اتنين
+
+645
+00:50:15,620 --> 00:50:20,680
+مرتبطين مع بعض هم البعض زي عاملين زي شكل النضارة و
+
+646
+00:50:20,680 --> 00:50:25,220
+الحبيبات لونها red و كبيرة، تمام؟ فهذه بسميها a
+
+647
+00:50:25,220 --> 00:50:29,000
+xenophil طيب ال bezophil kid بتكون .. بتكون ايضا
+
+648
+00:50:29,280 --> 00:50:33,020
+فيها two loops هايهم two loops غالبا ال most
+
+649
+00:50:33,020 --> 00:50:37,040
+common two loops تمام؟ و بتكون عليها حبيبات كبيرة
+
+650
+00:50:37,040 --> 00:50:40,400
+تمام؟ زي ما انتوا شايفين هذه الحبيبات من ال black
+
+651
+00:50:40,400 --> 00:50:46,300
+ل purple لونها و بيكون لونها ذامج و بنسميها او او
+
+652
+00:50:46,300 --> 00:50:49,500
+يعني بتكون مخدة شكل الجلاء النوع بتكون مخدة شكل
+
+653
+00:50:49,500 --> 00:50:54,820
+glass shape و بنحكي عن الحبيبات obscure nucleus اش
+
+654
+00:50:54,820 --> 00:50:56,760
+يعني obscure nucleus؟
+
+655
+00:50:58,980 --> 00:51:03,420
+هنا نحكيها basophil
+
+656
+00:51:03,420 --> 00:51:10,100
+granules .. granules
+
+657
+00:51:10,100 --> 00:51:17,280
+تمام obscure nucleus
+
+658
+00:51:20,410 --> 00:51:25,470
+تمام؟ obscure يعني أنها بتغطي النواة لدرجة أنه
+
+659
+00:51:25,470 --> 00:51:29,450
+احنا مش .. مش قادرين نشوف تفاصيل النواة تمام؟ لكن
+
+660
+00:51:29,450 --> 00:51:32,490
+احنا لو .. لو مافيش granules تكون .. هتكون النواة
+
+661
+00:51:32,490 --> 00:51:35,250
+عبارة ال most common أنها تكون two loops بس ممكن
+
+662
+00:51:35,250 --> 00:51:39,570
+تكون من اتنين لاربعة زي ال azenote تمام؟ و بيكونوا
+
+663
+00:51:39,570 --> 00:51:42,870
+مرتبط .. متصلين مع بعضهم البعض هيك بخطرة فيه و
+
+664
+00:51:42,870 --> 00:51:46,390
+عاملين زي شكل النضارة تمام؟ glass chip ال nucleus
+
+665
+00:51:46,390 --> 00:51:50,970
+تمام؟طيب إذا هى الـ neutrophil والإيزينوفل
+
+666
+00:51:50,970 --> 00:51:53,490
+والبيزوفل خلايا محببة زى ما أنتوا شايفين الـ
+
+667
+00:51:53,490 --> 00:51:57,410
+cytoplasm تبعها في حبيبها طيب نجى للـ agranulocyte
+
+668
+00:51:57,410 --> 00:52:00,190
+اللى هى الخلايا الغير محببة أو على الأقل الـ
+
+669
+00:52:00,190 --> 00:52:05,830
+monocyte احنا حكينا بنمشيها على انها هىأجران
+
+670
+00:52:05,830 --> 00:52:09,410
+يولوسايت ليش لإن الحبيبات تبعتها بتكونوش واضحة إلا
+
+671
+00:52:09,410 --> 00:52:11,610
+لازم أشوفها على electron microscope يعني على
+
+672
+00:52:11,610 --> 00:52:15,790
+microscope كتير قوة تكبيره عالية تمام يعني في ال
+
+673
+00:52:15,790 --> 00:52:18,050
+light microscope ال microscope العادي اللي إحنا
+
+674
+00:52:18,050 --> 00:52:22,030
+بنستخدمه صعب أشوف حبيبات ال ال monocyte عشان هيك
+
+675
+00:52:22,030 --> 00:52:25,930
+ممكن نحكي عنها تجاوزا إنها هي أجران يولوسايت لكن
+
+676
+00:52:25,930 --> 00:52:30,500
+اللي بديلكم تعرفوا إنه لأ هي فيها حبيباتطيب ما
+
+677
+00:52:30,500 --> 00:52:33,660
+علينا ال lymphocyte هي عبارة عن خلية صغيرة small
+
+678
+00:52:33,660 --> 00:52:40,540
+white pieces و النواة تبعتها round or oval تمام
+
+679
+00:52:40,540 --> 00:52:46,340
+بتكون إما دائرية أو بيضاوية يعني مقاربة لحجم ال
+
+680
+00:52:46,340 --> 00:52:50,130
+RBCsيعني لو انت شفتي خلية وشكاتي انها lymphocyte
+
+681
+00:52:50,130 --> 00:52:53,010
+على طول اطلعي على النواء تبعتها واطلعي على ال RBCs
+
+682
+00:52:53,010 --> 00:52:58,750
+اللي جنبها اذا لاجيتيهم متقاربين في الحجم معناها
+
+683
+00:52:58,750 --> 00:53:02,050
+انها هي على الأغلب lymphocyte ال cytoplasm تبعها
+
+684
+00:53:02,050 --> 00:53:09,650
+بيكون blue و ال grey و بتكون طبعا ال نواء شغل حجم
+
+685
+00:53:09,650 --> 00:53:14,320
+كبيرمن ال cytoplasm لدرجة انه بس عندي حلقة رفيعة
+
+686
+00:53:14,320 --> 00:53:17,180
+من ال cytoplasm شايفين هنا حلقة رفيعة من ال
+
+687
+00:53:17,180 --> 00:53:21,060
+cytoplasm هي اللي بتكون مبينة بنسميها thin rim of
+
+688
+00:53:21,060 --> 00:53:28,360
+cytoplasm اذا نيجي هنا تاني ال lymphocyte
+
+689
+00:53:28,360 --> 00:53:36,780
+lymphocyte has thin rim of
+
+690
+00:53:39,550 --> 00:53:40,590
+cytoplasm
+
+691
+00:53:43,420 --> 00:53:47,700
+طبعا فإذا ال lymphocyte لها هيك زي حلقة بسيطة من
+
+692
+00:53:47,700 --> 00:53:52,280
+ال cytoplasm بتكون موجودة لإن النواة محتلة معظم
+
+693
+00:53:52,280 --> 00:53:56,380
+حجم الخلية طبعا زي ما انتوا شايفين هنا هذه اللي هي
+
+694
+00:53:56,380 --> 00:53:59,480
+ال lymphocyte طب نيجي ل ال monocyte ال monocyte
+
+695
+00:53:59,480 --> 00:54:03,420
+غالبا بتكون ال kidney .. sorry النواة تبعتها
+
+696
+00:54:03,420 --> 00:54:06,960
+kidney shape طبعا زي شكل الكيلة زي شكل ال kidney
+
+697
+00:54:06,960 --> 00:54:09,640
+shape أو حدوة الفرس ولكن
+
+698
+00:54:11,840 --> 00:54:17,380
+طيب و لكن ال .. ال .. ممكن أنا أشوف monocyte
+
+699
+00:54:17,380 --> 00:54:21,020
+النواء تبعتها شكلها irregular يعني عادي برضه كمان
+
+700
+00:54:21,020 --> 00:54:24,880
+بيكون شكلها غير منتظم بعرفها إنها عبارة عن خلية
+
+701
+00:54:24,880 --> 00:54:29,440
+كتير كبيرة و النواء تبعتها غير منتظمة و
+
+702
+00:54:29,440 --> 00:54:35,130
+الcytoplasm تبعها فيه زي الغباشهنا نتيجة وجود ايه
+
+703
+00:54:35,130 --> 00:54:38,590
+مش حاكيه هنا ال Azirophil granule اللي هي مش واضحة
+
+704
+00:54:38,590 --> 00:54:43,130
+على ال ال light microscope طبعا فعشانها مش واضحة
+
+705
+00:54:43,130 --> 00:54:46,990
+بتكون ال cytoplasm مش نقي ولا pure زي مثلا هنا
+
+706
+00:54:46,990 --> 00:54:51,630
+طبعا فبيكون زي الغباش فيه هذا بسبب وجود ال
+
+707
+00:54:51,630 --> 00:54:56,660
+azirophil granule طبعاطيب طب أنا كيف دي أفرق ما
+
+708
+00:54:56,660 --> 00:55:00,500
+بين مثلا ال band و ما .. ما بين ال monocyte أول
+
+709
+00:55:00,500 --> 00:55:05,320
+حاجة في حال .. في حالة ال band الحبيبات إيه بتفرج؟
+
+710
+00:55:05,320 --> 00:55:07,900
+يعني لو كانت حبيباتها pink فهي band .. band
+
+711
+00:55:07,900 --> 00:55:12,160
+neutrophil لو كانت حبيباتها red معناها هي band
+
+712
+00:55:12,160 --> 00:55:16,700
+xenophil و لو كانت حبيباتها purple غامق لا blue لا
+
+713
+00:55:16,700 --> 00:55:21,880
+black فمعناها هي band bisophil تمام؟طيب، هذه شغلة
+
+714
+00:55:21,880 --> 00:55:25,760
+تانية، لكن أنه أنتوا زي .. شايفين النواة نفس الشكل
+
+715
+00:55:25,760 --> 00:55:31,020
+طيب، هلأ لو أنا جيت سكرت ال .. النواة و .. وكملتها
+
+716
+00:55:31,020 --> 00:55:36,440
+بشكل دائري، تمام؟ و رسمت خط وهمي من نهاية النواة
+
+717
+00:55:36,440 --> 00:55:43,110
+إلى ال .. ال .. ل هنا، تمام؟إذا كان الخط هذا هو
+
+718
+00:55:43,110 --> 00:55:48,970
+يعني أكبر من .. من نصف قطر الدائرة اللي أنا سكرتها
+
+719
+00:55:48,970 --> 00:55:54,130
+معناها دي band تمام؟ يعني مثلا بسكر و بحدد المركز،
+
+720
+00:55:54,130 --> 00:55:57,950
+نقطة المركز و برسم لو المسافة أكتر من نصف القطر
+
+721
+00:55:57,950 --> 00:56:03,570
+معناها أنه دي band تمام؟ لكن لو أنا هنا جيت سكرت و
+
+722
+00:56:03,570 --> 00:56:09,010
+رسمت خطتمام و على اعتبار انه مثلا نص القطر هنا ف
+
+723
+00:56:09,010 --> 00:56:13,490
+.. او المركز هنا هيكون فالمسافة اللي مفتوحة هذه
+
+724
+00:56:13,490 --> 00:56:17,450
+الفرزة هي عبارة عن مساوية او اقل من نص .. لا نصف
+
+725
+00:56:17,450 --> 00:56:22,690
+القطر فمعناها انه هذه خلية monocyte او نواة
+
+726
+00:56:22,690 --> 00:56:24,190
+monocyte مش نواة band
+
+727
+00:56:27,180 --> 00:56:31,120
+طيب إذا هيك بتكون تعرفنا على أشكال الخلايا طب
+
+728
+00:56:31,120 --> 00:56:34,860
+البلاتلت احنا حاكيناه اللي بلاتلت هي فتافيت من
+
+729
+00:56:34,860 --> 00:56:39,680
+خلية ال mega-karyocyte صح؟ تمام فال mega-karyocyte
+
+730
+00:56:39,680 --> 00:56:43,400
+هي خلية كبيرة موجودة في البون مارو لما بيتفتفت ال
+
+731
+00:56:43,400 --> 00:56:46,160
+cytoplasm تبعها وبيطلع ع ال circulation بيطلع على
+
+732
+00:56:46,160 --> 00:56:52,460
+هيئة البلاتلت اللي الصفائح أو الصفايحات تمام؟فهذه
+
+733
+00:56:52,460 --> 00:56:56,700
+الحبيبات أو النقاط اللي لونها purple هذه عبارة عن
+
+734
+00:56:56,700 --> 00:57:00,980
+بليتلت هي هنا هذه بليتلت تمام هذه بليتلت هذه
+
+735
+00:57:00,980 --> 00:57:05,520
+بنسميها بليتلت اللي هنا تكون موجودة ما بين ال RBCs
+
+736
+00:57:05,520 --> 00:57:09,080
+فبنسميها بليتلت هذا بليتلت aggregation تجمع من
+
+737
+00:57:09,080 --> 00:57:13,760
+البليتلت طيب ال RBCs اللي هو ال red blood cell زي
+
+738
+00:57:13,760 --> 00:57:19,950
+ما انتوا شايفين خلاياشكلها discotheque قرصي تمام و
+
+739
+00:57:19,950 --> 00:57:23,630
+ممكن تكون أخدت شكل هيكا oval أو إشي من الضغط لكن
+
+740
+00:57:23,630 --> 00:57:26,950
+هي شكلها قرصي أنا لو شيته طلعت عليها حكينا ال RBCs
+
+741
+00:57:28,050 --> 00:57:32,590
+لما أجى أطلع عليها من ال .. ال .. ال .. الجهة
+
+742
+00:57:32,590 --> 00:57:36,250
+العلوية بشوفها discotheque لكن لما أجى أطلع عليها
+
+743
+00:57:36,250 --> 00:57:39,690
+من side view بشوفها biconcave طبعا فإذا انهي ناجيت
+
+744
+00:57:39,690 --> 00:57:43,190
+أطلع عليها من فوق discotheque زي القرص طبعا زي
+
+745
+00:57:43,190 --> 00:57:47,570
+القرص مخدشك للقرص طبعا و فيها homoglobin ال
+
+746
+00:57:47,570 --> 00:57:50,990
+homoglobin يحتل أكتر من تلتينها يعني أكتر من 60%
+
+747
+00:57:50,990 --> 00:57:55,270
+من حجمهاوفي في المص منطقة فاضية هذه المنطقة
+
+748
+00:57:55,270 --> 00:57:59,530
+الفاضية بسميها ال central baller تمام شحوب تمام
+
+749
+00:57:59,530 --> 00:58:03,170
+central baller فهذا ال central baller يحتل طولة
+
+750
+00:58:03,170 --> 00:58:08,330
+الخلية central baller
+
+751
+00:58:08,330 --> 00:58:09,310
+of
+
+752
+00:58:11,880 --> 00:58:17,320
+RBC's طبعا؟ شوفوا إيش يعني؟ Central المركزي بعلر
+
+753
+00:58:17,320 --> 00:58:22,700
+الشحوب كلمة بعلر شحوب طبعا؟ of RBC's طيب ليش أنا
+
+754
+00:58:22,700 --> 00:58:26,820
+سميتها Central بعلر of RBC's؟ طلعوا معايا إحنا هل
+
+755
+00:58:26,820 --> 00:58:29,280
+جيت لما نحكي عن شخص شاحب إيش يعني؟ و الله بنقول و
+
+756
+00:58:29,280 --> 00:58:34,180
+الله دمه ضعيف و هو شاحبتمام؟ يعني مافيش عنده
+
+757
+00:58:34,180 --> 00:58:37,880
+homoglobin كفاية فنفس الاشي المنطقة هذه مافيش فيها
+
+758
+00:58:37,880 --> 00:58:41,660
+homoglobin فبالتالي بسميها شاحبة المنطقة الشاحبة
+
+759
+00:58:41,660 --> 00:58:45,300
+من الخلية لإن هي منطقة مافيش فيها homoglobin ومش
+
+760
+00:58:45,300 --> 00:58:49,020
+ماخدة لون ال homoglobin تمام؟ لكن لما بنحكي عن شخص
+
+761
+00:58:49,020 --> 00:58:53,580
+والله إنه متورد ليش؟ لإنه .. لإن مثلا الدم بيسر
+
+762
+00:58:53,580 --> 00:58:58,450
+عنده نتيجة وجود homoglobin تمام؟فإذا ارتبط دايما
+
+763
+00:58:58,450 --> 00:59:01,670
+مصطلح الشحوب في انه مافيش عنده homoglobin او انه
+
+764
+00:59:01,670 --> 00:59:05,810
+عنده دم مضريف او كذا فنفس الاشي ان هذه المنطقة
+
+765
+00:59:05,810 --> 00:59:10,350
+سميت بالبعلربش بالمنطقة الشاحبة المركزية اللي
+
+766
+00:59:10,350 --> 00:59:17,050
+بتحتال طول الخلية من طولة الحجم نظرا لإنها فراغ مش
+
+767
+00:59:17,050 --> 00:59:21,080
+موجود فيها homoglobinطب إيش موجود فيها؟ موجود فيها
+
+768
+00:59:21,080 --> 00:59:25,060
+cytoplasm ومكونات أخرى للخلية يعني أنزيمات
+
+769
+00:59:25,060 --> 00:59:30,400
+وبروتينات أخرى بتلزم الخلية في حياتها اللي هو خلال
+
+770
+00:59:30,400 --> 00:59:32,760
+ال 120 يوم رحلة حياتها
+
+771
+00:59:37,700 --> 00:59:44,760
+طيب أنا هيك سبقت ال .. ال .. الشريحة و اتعرفت كي
+
+772
+00:59:44,760 --> 00:59:49,740
+يعني .. أصير صار عندي فكرة على ال .. الخلايا و
+
+773
+00:59:49,740 --> 00:59:55,820
+أجزائها و كيف هيكون شكلها بعد ما تاخد الصباغة و ..
+
+774
+00:59:55,820 --> 01:00:00,030
+هلأ بدي أنا أشوفها تحت ال microscopeبدي اجيب ال
+
+775
+01:00:00,030 --> 01:00:06,130
+field على 10x بعدين احول على 100x طبعا ال 100x انا
+
+776
+01:00:06,130 --> 01:00:10,430
+محتاجة ان انا احط قطرة زيت علشان اشوف بشكل واضح
+
+777
+01:00:10,430 --> 01:00:14,770
+طبعا طيب ممكن اعمل differential اش يعني
+
+778
+01:00:14,770 --> 01:00:18,510
+differential يعني اشوف اعد 100 خلية من ال white
+
+779
+01:00:18,510 --> 01:00:24,390
+pieces وكل ما بعيد خلية بعرف بجيب نوعها طبعا او
+
+780
+01:00:24,390 --> 01:00:28,080
+يعني بحدد نوعهافي النهاية بعد ما أكون عديت 100
+
+781
+01:00:28,080 --> 01:00:31,820
+خلية، بدي أشوف من المية هدولة كم كانوا monocyte،
+
+782
+01:00:31,820 --> 01:00:34,200
+كم كانوا neutrophil، أه كم كانوا eosinophil أو
+
+783
+01:00:34,200 --> 01:00:38,520
+bezophil أو يعني الخلايا الأخرى، فبالتالي هيك بكون
+
+784
+01:00:38,520 --> 01:00:42,220
+أنا عملت differential، جيبت نسبة كل نوع من أنواع
+
+785
+01:00:42,220 --> 01:00:46,740
+ال white species من أصل 100 خلية أنا عديتهم، تمام؟
+
+786
+01:00:47,580 --> 01:00:52,400
+طيب بس انتوا هالده مش .. يعني في المرحلة الحالية
+
+787
+01:00:52,400 --> 01:00:55,120
+مش مطلوب منكم تعملوا differential هتعملوا ان شاء
+
+788
+01:00:55,120 --> 01:00:59,620
+الله بس تروحوا على ال hematology لكن المطلوب منكم
+
+789
+01:00:59,620 --> 01:01:02,720
+حاليا انكوا تكونوا بتعرفوا تجيبوا ال field بتعرفوا
+
+790
+01:01:02,720 --> 01:01:07,500
+ايه الأساسيات الميكروسكوب وكيف تستكشف الشريحة كيف
+
+791
+01:01:07,500 --> 01:01:10,720
+تتعرفي على شكل الخلايا يعني هادي RBCs هادي بلاتلت
+
+792
+01:01:10,720 --> 01:01:13,140
+هادي lymphocyte , monocyte, azenophile,
+
+793
+01:01:13,360 --> 01:01:19,450
+neutrophil, bezophil و هكذا تمام؟طيب أنا بدي أجيب
+
+794
+01:01:19,450 --> 01:01:23,690
+ال field على عشرة طبعا اللي هي مثلا الشريحة تبعتي
+
+795
+01:01:23,690 --> 01:01:28,050
+و عليها ال blood film اللي أنا فردته طبعا لازم أنا
+
+796
+01:01:28,050 --> 01:01:31,410
+لما أعيش معانا أ center الشريحة يعني بدي أحط
+
+797
+01:01:31,410 --> 01:01:35,050
+المنطقة القراءة اللي بدي أقرأها بدي يكون تحتها ال
+
+798
+01:01:35,050 --> 01:01:40,870
+condenser و فوقها مباشرة اللي .. العدسة طبعا؟ طيب
+
+799
+01:01:40,870 --> 01:01:47,030
+فالمنطقة اللي يتقرأ هي منطقة ال .. ال ..ال .. ال
+
+800
+01:01:47,030 --> 01:01:53,030
+.. يعني الطرف الرافيع أو المنطقة ال thin في ال
+
+801
+01:01:53,030 --> 01:01:58,070
+blood smear تمام؟ هذه هي المنطقة اللي أنا بقراها
+
+802
+01:01:58,070 --> 01:02:01,710
+فإذا هذه المنطقة اللي بدي يكون تحتها ال condenser
+
+803
+01:02:01,710 --> 01:02:08,400
+و فوقها العدسةطيب و إذا ب .. بحط عدسة عشرة on و
+
+804
+01:02:08,400 --> 01:02:12,520
+ببدأ أرفع ال stage شوي شوي لحد ما أشوف الخلايا بعد
+
+805
+01:02:12,520 --> 01:02:17,720
+هيك بروح بدور على منطقة في .. يعني على .. يعني ..
+
+806
+01:02:17,720 --> 01:02:21,920
+مكانفي هذه المنطقة انه يكون الخلايا evenly
+
+807
+01:02:21,920 --> 01:02:25,900
+distributed اش يعني evenly distributed؟ يعني انه
+
+808
+01:02:25,900 --> 01:02:32,900
+تكون الخلايا موزعة بشكل منيح و تكون متباعدة شوي و
+
+809
+01:02:32,900 --> 01:02:38,020
+مش يعني مش متراكبة على بعض هالبعض و مافيش زحمة و
+
+810
+01:02:38,020 --> 01:02:42,720
+تكون عشان انا أتعرف على شكل الخلايا بشكل واضح و
+
+811
+01:02:42,720 --> 01:02:50,670
+أصنفها بشكل دقيق تمام؟طيب و بقيم من خلالها ال RBCs
+
+812
+01:02:50,670 --> 01:02:54,990
+distribution شكلها عاملة role information مش عاملة
+
+813
+01:02:54,990 --> 01:02:59,870
+role ال .. الخلايا ال homoglobin تبعها كويس ولا
+
+814
+01:02:59,870 --> 01:03:03,250
+فاضي اذا طبعا كيف ب .. ب .. بحكم اذا ال central
+
+815
+01:03:03,250 --> 01:03:06,170
+barrier هذا محتل اكتر من طولة الخلية معناها انها
+
+816
+01:03:06,170 --> 01:03:08,490
+خلية فاضية و مافيش فيها homoglobin يعني الشخص عنده
+
+817
+01:03:08,490 --> 01:03:14,250
+anemia طيب
+
+818
+01:03:16,450 --> 01:03:21,910
+و بعد هيك بحط قطرة زيت و بحول عالمية و ماتنسوش انه
+
+819
+01:03:21,910 --> 01:03:26,050
+احنا بنفتح القرص في هاي اللحظة علشان انا بدي إضاءة
+
+820
+01:03:26,050 --> 01:03:31,190
+عالية و ببدأ أفحص العينة بشكل systematic بشكل زيك
+
+821
+01:03:31,190 --> 01:03:34,710
+زاج طبعا احنا كمان حكينا انه العد برضه كمان بيكون
+
+822
+01:03:34,710 --> 01:03:38,110
+بشكل زيك زاج اذا بروح من الطرف اللي أنا ببدأ و
+
+823
+01:03:38,110 --> 01:03:44,010
+بفوت لجوة بهذا الشكل طبعا زيك زاجو بكون انا بعد
+
+824
+01:03:44,010 --> 01:03:48,990
+هيك استكشفت الخلية طبعا او استكشفت اشكال الخلايا و
+
+825
+01:03:48,990 --> 01:03:52,830
+اتعرفت عليها ال .. هاي ال bezelfield شايفين؟ two
+
+826
+01:03:52,830 --> 01:03:57,590
+loops طبعا و الحبيبات كبيرة مغطية الأنوية او يعني
+
+827
+01:03:57,590 --> 01:04:00,910
+موجودة .. احيانا بتكون مغطية كل ال .. كل الأنوية
+
+828
+01:04:00,910 --> 01:04:04,850
+ما .. يعني مابيبين تفاصيل النواء منها ال
+
+829
+01:04:04,850 --> 01:04:08,390
+neutrophil حبيباتها ناعم و لونها pink و هاي شايفين
+
+830
+01:04:08,390 --> 01:04:09,370
+هاي أربعة loops
+
+831
+01:04:12,390 --> 01:04:16,210
+ممكن يكونوا خمسة يعني ممكن يكون هذا الخامس هنا
+
+832
+01:04:16,210 --> 01:04:21,470
+isenophile هاي تلاتة loops وحبيبتها لونها أحمر هاي
+
+833
+01:04:21,470 --> 01:04:26,890
+lymphocyte طبعا اللي حكينا إنه في عندي thin rim of
+
+834
+01:04:26,890 --> 01:04:30,150
+cytoplasm وهي هنا ال monocyte اللي هي النواة
+
+835
+01:04:30,150 --> 01:04:38,590
+kidney shape وهنا herpesis تمام؟ بهذا الشكلال RBCs
+
+836
+01:04:38,590 --> 01:04:45,070
+اللي يعني ما بيكون إليها central pattern بتكون
+
+837
+01:04:45,070 --> 01:04:51,530
+spherocyte يعني مخدة شكل كوروي و ليس ديسكوتيك طبعا
+
+838
+01:04:51,530 --> 01:04:58,550
+طيب هلأ أنا إيش بدي أعمل مطلوب مني يعني أعرف إيش
+
+839
+01:04:58,550 --> 01:05:02,730
+فكرة ال differential differential
+
+840
+01:05:02,730 --> 01:05:04,670
+طبعا
+
+841
+01:05:09,560 --> 01:05:13,480
+طيب إيش يعني differential؟ حكينا معنى كلمة
+
+842
+01:05:13,480 --> 01:05:30,880
+differential إنه أنا .. لحظة شوف .. ليش ..
+
+843
+01:05:30,880 --> 01:05:32,140
+ما بعرف ليش ..
+
+844
+01:05:42,090 --> 01:05:46,790
+طبعا، هلأ أنا بدي أروح أجيب يعني و أنا ب .. أبعمل
+
+845
+01:05:46,790 --> 01:05:51,810
+scanning و مسح لل .. لل blood film و بفحصه بشكل
+
+846
+01:05:51,810 --> 01:05:57,190
+zigzag زي هيك، طبعا، بدي أكون بعد ميت خلية white
+
+847
+01:05:57,190 --> 01:06:00,950
+pieces و في نفس الوقت الميت خلية هدول مثلا بدي
+
+848
+01:06:00,950 --> 01:06:05,430
+أكون أنا على الورقام صنفاهم يعني على ال .. ال
+
+849
+01:06:05,430 --> 01:06:08,090
+report اللي .. أو المكان اللي أنا بدي أسجل فيه
+
+850
+01:06:08,090 --> 01:06:10,590
+نتائج تبعتي، مثلا cat بالينفا
+
+851
+01:06:18,200 --> 01:06:25,120
+lympho mono neutro
+
+852
+01:06:25,120 --> 01:06:34,960
+ايو زينو طبعا انا بس باختصر الكلمات على أساس انه
+
+853
+01:06:34,960 --> 01:06:40,620
+ماأطول في الكتابة بس يعني انه lymphocyte,
+
+854
+01:06:40,700 --> 01:06:45,180
+monocyte, neutrophil, xenophil and bisophil تمام؟
+
+855
+01:06:45,330 --> 01:06:50,150
+طيب، هلأ مثلا أنا و أنا بعد لأ وجدت اتنين ..
+
+856
+01:06:50,150 --> 01:06:54,530
+اتنتين lymphocyte بروح بحط مثلا زي هيك تمام، هي
+
+857
+01:06:54,530 --> 01:07:00,490
+عصيتين، مثلا وجدت واحدة monocyte، هي زي هيك تمام
+
+858
+01:07:00,490 --> 01:07:05,150
+ال neutrophil، ماشيت مثلا على field تاني، طبعا أنا
+
+859
+01:07:05,150 --> 01:07:10,740
+بكون بعمل scanning، ماشية، بتحرك، هيكو نزلت و كمان
+
+860
+01:07:10,740 --> 01:07:14,660
+لفت و نزلت و لفت و هكذا تمام؟ أثناء اللعب مثلا
+
+861
+01:07:14,660 --> 01:07:22,580
+وجدت تلاتة lymphocyte فهي .. هاي تلاتة مثلا ال ..
+
+862
+01:07:22,580 --> 01:07:31,600
+وجدت مثلا واحدة xenofel وجدت مثلا zerobezofel رجعت
+
+863
+01:07:31,600 --> 01:07:36,580
+هنا تاني كمان و روحت على field أخر مثلا وجدت أنه
+
+864
+01:07:36,580 --> 01:07:46,410
+في عندي ..مثلا تنتين lymph تنتين mono تنتين
+
+865
+01:07:46,410 --> 01:07:50,590
+neutral زي هيك بروح بحط الرابعة و الخامسة بحطها
+
+866
+01:07:50,590 --> 01:07:55,030
+بشكل مائل يعني بسكر فيها ال .. الأربعة دول كل خمسة
+
+867
+01:07:55,030 --> 01:07:58,810
+.. كل أربعة الخمسة بتسكرهم يعني زي الحزم في
+
+868
+01:07:58,810 --> 01:08:03,840
+النهاية عدد الخلايا هنا بديكون مية خليةو ال
+
+869
+01:08:03,840 --> 01:08:07,920
+lymphocyte في الآخر عدد العصيات اللي أنا حطيتهم أو
+
+870
+01:08:07,920 --> 01:08:13,760
+عدد الخلايا اللي أنا عديتهم هو عبارة عن نسبة
+
+871
+01:08:13,760 --> 01:08:17,560
+المئوية لل lymphocyte لإن هو عدد الخلايا خلال 100
+
+872
+01:08:17,560 --> 01:08:21,820
+خليةطبعا و نفس الاشي للمونو للنيوترو والإيزينو طيب
+
+873
+01:08:21,820 --> 01:08:26,160
+ال .. ال .. الوضع الطبيعي طبعا من .. من تقريبا من
+
+874
+01:08:26,160 --> 01:08:30,860
+15 إلى 20 أو إلى 25 نسبة طبيعية لل lymphocyte ال
+
+875
+01:08:30,860 --> 01:08:36,140
+monocyte مثلا من 8 ل 10% أو ل 15% لل neutral من 60
+
+876
+01:08:36,140 --> 01:08:40,760
+ل 70% هو ال normal .. ال normal ال eizinophil من 1
+
+877
+01:08:40,760 --> 01:08:46,510
+إلى 2%أو حتى كمان أحيانا بيحكوا أنه من 0 إلى 2%
+
+878
+01:08:46,510 --> 01:08:50,450
+لإنه ممكن ما أشوفش ولا إزينو و ال basophil أيضا
+
+879
+01:08:50,450 --> 01:08:55,430
+نفس الأشي من 0 إلى 1 أو إلى 2% برضه كمان لإنه ال
+
+880
+01:08:55,430 --> 01:08:58,990
+basophil يعني كمان برضه كمان نادر لما أشوفها، هلأ
+
+881
+01:08:58,990 --> 01:09:02,890
+ال lymphocyte وظيفتها إنها بتحافظ على الخلق يعني
+
+882
+01:09:02,890 --> 01:09:07,690
+بت .. بتهاجم ال .. ال virusتمام؟ هي بتحافظ على
+
+883
+01:09:07,690 --> 01:09:11,890
+الجسم من ال viruses من ال viral infection ال
+
+884
+01:09:11,890 --> 01:09:15,670
+monocyte بتلتهم الخلايا بتتحول طبعاً ل macrophage
+
+885
+01:09:15,670 --> 01:09:19,610
+و بتروح تلتهم الخلايا الميتة أو الخلايا السرطانية
+
+886
+01:09:19,610 --> 01:09:24,030
+أو حتى ال .. البكتيريا و بتقتلها ال neutrophil بت
+
+887
+01:09:24,030 --> 01:09:27,770
+.. طبعاً هي بتهاجم ضد البكتيريا يعني ضد ال
+
+888
+01:09:27,770 --> 01:09:31,660
+bacterial infectionتمام ال azinophil ضد ال
+
+889
+01:09:31,660 --> 01:09:34,680
+parasite ضد الطفاليات و أيضا إلها دور في ال
+
+890
+01:09:34,680 --> 01:09:38,320
+allergy و ال bisophil كمان إلها دور في ال allergy
+
+891
+01:09:38,320 --> 01:09:42,110
+و في ال antigen representationيعني في تقديم ال
+
+892
+01:09:42,110 --> 01:09:47,210
+antigen للخلايا التانية عشان انها تقتله تمام؟ هذا
+
+893
+01:09:47,210 --> 01:09:52,410
+وظائف الخلايا او حسب يعني .. او تخصصها في الدفاع
+
+894
+01:09:52,410 --> 01:09:57,730
+عن جسم الإنسان طيب لو كان في عندي زيادة في ال
+
+895
+01:09:57,730 --> 01:10:02,050
+lymphocyte ايش بسميها هذا المصطلح؟ بسميه
+
+896
+01:10:02,050 --> 01:10:10,000
+lymphocytetosis تمام lymphocytosis وإذا كانت في
+
+897
+01:10:10,000 --> 01:10:15,160
+عندي طبعا قلة في ال lymphocyte أو نقص في عدد ال
+
+898
+01:10:15,160 --> 01:10:20,900
+lymphocyte فمعناها هذا بسميه lympho
+
+899
+01:10:20,900 --> 01:10:29,400
+lymphocytopenia
+
+900
+01:10:29,400 --> 01:10:35,450
+lymphocytopeniaعلى وزم ليكوبينيا حكينا الليكوبينيا
+
+901
+01:10:35,450 --> 01:10:39,310
+يعني قلة في ال white pieces ككل و اللي .. و ال ..
+
+902
+01:10:39,310 --> 01:10:42,990
+ليكو سايتوسز يعني زيادة في ال white pieces ككل و
+
+903
+01:10:42,990 --> 01:10:49,110
+هاد .. هاد الاشي ذكرناه في محاضرة ال .. ال white
+
+904
+01:10:49,110 --> 01:10:53,010
+pieces، تمام؟ و ذكرناه أيضا في .. في فحص ال
+
+905
+01:10:53,010 --> 01:10:57,890
+homoglobin تمام،
+
+906
+01:10:57,890 --> 01:11:02,650
+monocytosisنفس فكرة ال lymphocyte ما هي اصلا كلمة
+
+907
+01:11:02,650 --> 01:11:06,570
+lymphocyte تنتهي ب site و ال monocyte تنتهي ب site
+
+908
+01:11:06,570 --> 01:11:12,090
+فعشان هم بينتهوا زي بعض فهيكون
+
+909
+01:11:12,090 --> 01:11:16,670
+المصطلح اللي بيعبر عن جامعهم او عن زيادتهم او
+
+910
+01:11:16,670 --> 01:11:20,230
+نقصانهم نفس الاشي مثلا زيادة ال lymphocyte
+
+911
+01:11:20,230 --> 01:11:26,110
+lymphocytosis زيادة ال monocyte monocytosis
+
+912
+01:11:28,340 --> 01:11:38,620
+طبعا نقف ال monocyte هيكون monocytopenia
+
+913
+01:11:38,620 --> 01:11:45,160
+.. monocytopenia طبعا طيب ال .. ال neutral في
+
+914
+01:11:45,160 --> 01:11:51,660
+الزيادتها neutrophilia
+
+915
+01:11:51,660 --> 01:11:56,660
+.. طبعا neutrophilia و نقصانها
+
+916
+01:12:04,910 --> 01:12:08,250
+neutropenia نفس الأشي ال azenophil زيادتها
+
+917
+01:12:08,250 --> 01:12:14,370
+azenophilia ونقصانها azenopenia, bezophilia,
+
+918
+01:12:14,770 --> 01:12:20,550
+bezocytopenia طبعا فبالتالي لازم هذه الشغلات أنتوا
+
+919
+01:12:20,550 --> 01:12:22,890
+تكونوا بتعرفوها أو المصطلحات تكونوا بتعرفوها و
+
+920
+01:12:22,890 --> 01:12:25,570
+تكونوا بتعرفوا إيش فكرة ال difference يعني طبعا
+
+921
+01:12:25,570 --> 01:12:30,030
+لكن أنهفعليا تفاصيل ال differential إيش مطلوب منكم
+
+922
+01:12:30,030 --> 01:12:35,010
+أو كيف أنه تعملوا هذا تاخدوا في ال hematology
+
+923
+01:12:35,010 --> 01:12:43,870
+تمام؟ بهيك بنكون وصلنا لنهاية محاضرتنا لليوم ان
+
+924
+01:12:43,870 --> 01:12:46,570
+شاء الله تكون واضحة وسهلة بالنسبة لإلكم ويعطيكم
+
+925
+01:12:46,570 --> 01:12:47,390
+ألف عافية
+
diff --git a/PL9fwy3NUQKwY9jl8xw-bWkVZkWcGGyspd/Eut_8KsMs84.srt b/PL9fwy3NUQKwY9jl8xw-bWkVZkWcGGyspd/Eut_8KsMs84.srt
new file mode 100644
index 0000000000000000000000000000000000000000..c14c458b7dddb0a957d848ae482fd620f99dacf8
--- /dev/null
+++ b/PL9fwy3NUQKwY9jl8xw-bWkVZkWcGGyspd/Eut_8KsMs84.srt
@@ -0,0 +1,2712 @@
+1
+00:00:04,860 --> 00:00:08,240
+السلام عليكم ورحمة الله وبركاته طالبات العزيزات
+
+2
+00:00:08,240 --> 00:00:10,600
+اليوم إن شاء الله راح نشرح محاضرة بعنوان
+
+3
+00:00:10,600 --> 00:00:14,780
+microscope راح نتعرف على الـ microscope، أجزاءه
+
+4
+00:00:14,780 --> 00:00:20,080
+وظائف هذه الأجزاء، وأيضاً أنواع الـ microscope، وطريقة
+
+5
+00:00:20,080 --> 00:00:25,120
+استخدام الـ microscope، وطريقة العناية به، ومبدأ عمل
+
+6
+00:00:25,120 --> 00:00:29,470
+الـ microscope. في البداية، الـ microscope هو عبارة عن
+
+7
+00:00:29,470 --> 00:00:34,470
+is an important device that enables us to visualize
+
+8
+00:00:34,470 --> 00:00:39,430
+minute objects. هو عبارة عن جهاز مهم
+
+9
+00:00:39,430 --> 00:00:46,550
+device that enables us تمكننا من رؤية أو إيضاح الـ
+
+10
+00:00:46,550 --> 00:00:51,630
+minute objects، الأجزاء أو المكونات أو العناصر
+
+11
+00:00:51,630 --> 00:00:55,490
+الدقيقة جداً في العينة، سواء كانت هذه العناصر
+
+12
+00:00:55,490 --> 00:01:01,730
+حية أو غير حية، وهي الـ objects.
+
+13
+00:01:01,730 --> 00:01:07,190
+طبعاً لا نتمكن من رؤيتها بالعين المجردة، لذلك نستعين
+
+14
+00:01:07,190 --> 00:01:10,470
+بالـ microscope. نراها تحت الـ microscope that you
+
+15
+00:01:10,470 --> 00:01:14,730
+can't be seen by our naked eye. لذلك لا يمكن رؤيتها
+
+16
+00:01:14,730 --> 00:01:19,510
+بالعين المجردة. نراها تحت الـ microscope. إذاً هو
+
+17
+00:01:19,510 --> 00:01:22,770
+الأداة التي تمكننا من رؤية الـ minor objects، سواء
+
+18
+00:01:22,770 --> 00:01:25,530
+كانت هذه الـ objects حية أو غير حية، سواء كانت
+
+19
+00:01:25,530 --> 00:01:29,510
+المكونات أو العناصر أو الأجزاء المراد إيضاحها حية
+
+20
+00:01:29,510 --> 00:01:34,410
+أو غير حية. بداية هذا هو الـ microscope. مما يتكون الـ
+
+21
+00:01:34,410 --> 00:01:39,410
+microscope؟ أول شيء يتكون من ocular lenses، تمام؟
+
+22
+00:01:39,410 --> 00:01:43,050
+ocular lenses أو eyepiece، نفس المعنى، سواء حكينا
+
+23
+00:01:43,050 --> 00:01:50,110
+eyepiece أو ocular lenses، نفس المعنى. طيب، وأيضاً
+
+24
+00:01:50,110 --> 00:01:56,170
+مكون من الـ tube، وهذه الـ tube هي التي تنقل الصورة
+
+25
+00:01:56,170 --> 00:01:59,990
+التي تأخذها العدسات الشيئية، وتوديها على
+
+26
+00:01:59,990 --> 00:02:06,730
+العدسات العينية، عشان يتم تكبيرها، تمام؟ والمكون من
+
+27
+00:02:06,730 --> 00:02:15,470
+ذراع arm، هذا الـ arm. طيب، مكون أيضاً من base، قاعدة. مكون
+
+28
+00:02:15,470 --> 00:02:19,310
+من course adjustment، العجل هذا الكبير، اسمه course
+
+29
+00:02:19,310 --> 00:02:22,950
+adjustment، والعجل الصغير fine adjustment. طبعاً
+
+30
+00:02:22,950 --> 00:02:26,090
+موجودين، ها هما course adjustment و fine adjustment.
+
+31
+00:02:26,090 --> 00:02:32,190
+طيب، مكون من light source، و iris diaphragm، و
+
+32
+00:02:32,190 --> 00:02:35,910
+condenser، وهذه stage، هذه المنصة أو الطاولة التي
+
+33
+00:02:35,910 --> 00:02:40,470
+نحط عليها العينة. العينة يتم تثبيتها من خلال ملاقط
+
+34
+00:02:40,470 --> 00:02:46,600
+نسميها clips أو stage clips، تمام؟ هذه الـ
+
+35
+00:02:46,600 --> 00:02:49,180
+objective lenses، التي هي العدسات الشيئية التي..
+
+36
+00:02:49,180 --> 00:02:52,600
+التي هي تكبر الشيء المراد فحصه، التي.. التي..
+
+37
+00:02:52,600 --> 00:02:57,860
+التي نكون حاطينه في شريحة على الـ stage هنا، تمام؟
+
+38
+00:02:57,860 --> 00:03:02,080
+فإذاً هذه هي مكونات الـ.. الـ microscope. راح نتحدث
+
+39
+00:03:02,080 --> 00:03:06,220
+عنها بالتفصيل. بداية، خلونا لما بنحكي عن الـ major
+
+40
+00:03:06,220 --> 00:03:08,940
+parts of microscope، الأجزاء الأساسية في الـ
+
+41
+00:03:08,940 --> 00:03:13,360
+microscope، نقسمهم إلى أجزاء. أول شيء، خلونا نحكي عن
+
+42
+00:03:13,360 --> 00:03:16,720
+الهيكل، الـ framework of microscope، الشكل الخارجي للـ
+
+43
+00:03:16,720 --> 00:03:21,280
+microscope، مما يتكون.. يتكون من ثلاث قطع أو ثلاث
+
+44
+00:03:21,280 --> 00:03:26,000
+مناطق، تمام؟ الشكل الخارجي للـ microscope، أول شيء الـ
+
+45
+00:03:26,000 --> 00:03:30,480
+arm، هذه التي أنتم شايفينها، هذه الـ arm، هي التي
+
+46
+00:03:30,480 --> 00:03:36,370
+توصل الـ.. الـ.. الـ.. جسم الـ microscope ببعضه.
+
+47
+00:03:36,370 --> 00:03:42,550
+إذاً هي التي تعمل attachment أو اتصال أو ربط ما بين
+
+48
+00:03:42,550 --> 00:03:49,500
+الذي هو العدسات والـ stage والقاعدة، تمام؟ فإذاً هي
+
+49
+00:03:49,500 --> 00:03:53,380
+الجزء الأساسي في، أو التي هي عماد الـ microscope،
+
+50
+00:03:53,380 --> 00:03:58,260
+التي عليها يتم إيصال الأجزاء كلها ببعضها البعض. الـ
+
+51
+00:03:58,260 --> 00:04:02,580
+stage، هو عبارة عن الـ table، الطاولة هذه أو المنصة
+
+52
+00:04:02,580 --> 00:04:05,960
+هذه التي عليها نحن نحط الـ slide، والـ slide يكون
+
+53
+00:04:05,960 --> 00:04:10,320
+عليها الشريحة، أو هي الـ slide هي الشريحة التي يكون
+
+54
+00:04:10,320 --> 00:04:15,780
+عليها العينة المراد فحصها. Foot أو base، القدم أو
+
+55
+00:04:15,780 --> 00:04:20,120
+القاعدة، هي القاعدة المستطيلة التي الجهاز كله
+
+56
+00:04:20,120 --> 00:04:23,760
+يرتاح عليها، ويجلس عليها، ويستند عليها.
+
+57
+00:04:23,760 --> 00:04:28,280
+طبعاً، فإذاً لو أنا بدي أقسمه، أقسمه إلى ثلاث مناطق،
+
+58
+00:04:29,690 --> 00:04:33,790
+تمام؟ والـ.. الـ stage، والـ base. هذا هو الهيكل
+
+59
+00:04:33,790 --> 00:04:36,670
+الخارجي للـ microscope، the frame work أو التي
+
+60
+00:04:36,670 --> 00:04:41,590
+هي the framework of the microscope. طيب، نشوف الجهاز
+
+61
+00:04:41,590 --> 00:04:46,670
+أو الـ.. الـ.. الـ.. الـ system الثاني من تركيب الـ
+
+62
+00:04:46,670 --> 00:04:50,810
+microscope، الذي هو الـ focusing system، نظام التوضيح
+
+63
+00:04:50,810 --> 00:04:53,830
+في الـ microscope. إحنا حكينا أن الـ microscope يوضح،
+
+64
+00:04:53,830 --> 00:04:58,150
+يكبر، بيمكنني من رؤية الأجزاء التي هي لا ترى بالعين
+
+65
+00:04:58,150 --> 00:05:03,300
+المجردة. طيب، أكيد عنده نظام توضيح ونظام تكبير خاص
+
+66
+00:05:03,300 --> 00:05:08,200
+يعرض الصور بدقة متناهية. إذاً الـ.. الـ focusing
+
+67
+00:05:08,200 --> 00:05:12,720
+system، هو نظام الـ.. الـ.. الـ.. التوضيح أو
+
+68
+00:05:12,720 --> 00:05:17,810
+التركيز في الـ microscope، وهذا النظام يتكون من
+
+69
+00:05:17,810 --> 00:05:23,910
+عجلين، تمام، two knobs. هذان الـ two knobs نسميهما
+
+70
+00:05:23,910 --> 00:05:29,030
+الكبير نسميه course focusing adjustment، والصغير
+
+71
+00:05:29,030 --> 00:05:33,200
+fine focusing adjustment. للاختصار نحكي course
+
+72
+00:05:33,200 --> 00:05:38,800
+adjustment و fine adjustment، تمام؟ إذاً مكون من two
+
+73
+00:05:38,800 --> 00:05:43,600
+knobs، هذان هما pair of knobs، والكبير course
+
+74
+00:05:43,600 --> 00:05:47,300
+adjustment، والصغير fine adjustment. طيب، ما هو
+
+75
+00:05:47,300 --> 00:05:49,780
+وظيفة الـ course adjustment والـ fine adjustment؟
+
+76
+00:05:49,780 --> 00:05:54,350
+شوفوا معي. الـ course adjustment، خلّيني أرجع على الـ
+
+77
+00:05:54,350 --> 00:05:58,870
+microscope هنا. الـ course، ها هما هذان العجلين أو
+
+78
+00:05:58,870 --> 00:06:02,610
+القضيبان، هذان نسميهما course adjustment الكبير، و
+
+79
+00:06:02,610 --> 00:06:07,890
+الصغير fine adjustment. طيب، إيش وظيفة الـ course
+
+80
+00:06:07,890 --> 00:06:10,910
+adjustment؟ شوفوا معي، أنا لما أحط الشريحة هنا،
+
+81
+00:06:10,910 --> 00:06:15,810
+التي عليها العينة، محتاجة أقرب المسافة ما بين
+
+82
+00:06:15,810 --> 00:06:19,770
+العدسات الشيئية التي ترى الشيء، تمام، أو تكبر الشيء
+
+83
+00:06:19,770 --> 00:06:23,930
+المراد فحصه، وما بين الطاولة أو المنصة أو الـ stage
+
+84
+00:06:23,930 --> 00:06:28,310
+الذي موجود عليه هذه العينة، تمام؟ محتاجة أن المسافة
+
+85
+00:06:28,310 --> 00:06:33,790
+تكون قريبة، عشان يتم تكبير الـ.. الـ.. الـ.. العنصر،
+
+86
+00:06:33,790 --> 00:06:40,430
+وأيضاً يتم عرضه بإيضاح، يتم عرضه بإيضاح. طيب، إذاً
+
+87
+00:06:40,430 --> 00:06:45,090
+لا بد من أنه يا إما العدسات الشيئية تقترب من الـ
+
+88
+00:06:45,090 --> 00:06:50,130
+stage، أو الـ stage يقترب من العدسات الشيئية، تمام؟
+
+89
+00:06:51,210 --> 00:06:56,270
+فبتحريك هذا الـ knobs، الذي هو الـ.. الـ.. الـ course
+
+90
+00:06:56,270 --> 00:07:01,330
+adjustment، تمام؟ بتحريك هذا الـ knob يتم إما تقريب
+
+91
+00:07:01,330 --> 00:07:04,850
+العدسات الشيئية من الـ stage، أو تقريب الـ stage من
+
+92
+00:07:04,850 --> 00:07:07,880
+العدسات الشيئية. تمام، ستشوفون في مايكروسكوبات
+
+93
+00:07:07,880 --> 00:07:11,780
+العدسات الشيئية هي التي تتحرك، وفي مايكروسكوبات لأ،
+
+94
+00:07:11,780 --> 00:07:14,220
+الـ stage التي تتحرك. أنتم ستشتغلون تقريباً معظم
+
+95
+00:07:14,220 --> 00:07:17,440
+مايكروسكوبات التي عندنا بالقسم، هو الـ stage هو الذي
+
+96
+00:07:17,440 --> 00:07:23,080
+يتحرك، يصعد بالقرب من العدسات الشيئية. تقترب
+
+97
+00:07:23,080 --> 00:07:26,940
+العدسات الشيئية من الشريحة التي موجودة عليها
+
+98
+00:07:26,940 --> 00:07:31,620
+العينة المراد فحصها، وبالتالي، وبالتالي تكون
+
+99
+00:07:31,620 --> 00:07:36,220
+العدسات الشيئية قريبة من الـ objects، تمام، to be
+
+100
+00:07:36,220 --> 00:07:40,120
+visualized، التي نحن بدنا نوضحها. فتلتقط الصورة
+
+101
+00:07:40,120 --> 00:07:44,940
+المكبرة، وهذه الصورة المكبرة تنتقل عبر الـ tube أو
+
+102
+00:07:44,940 --> 00:07:50,550
+القناة التي هنا إلى العدسات الـ.. العينية، يتم
+
+103
+00:07:50,550 --> 00:07:55,210
+تكبيرها مرة أخرى. وإذا نحن نأخذ الـ.. الصورة
+
+104
+00:07:55,210 --> 00:07:58,950
+النهائية، تكون مكبرة مرتين، مرة بقوة تكبير العدسات
+
+105
+00:07:58,950 --> 00:08:02,710
+الشيئية أو العدسة الشيئية المستخدمة، والـ.. التي هي
+
+106
+00:08:02,710 --> 00:08:06,470
+تكون صورة أولية، والصورة الأولية هذه تنتقل إلى
+
+107
+00:08:06,470 --> 00:08:10,990
+العدسات العينية، ويتم تكبيرها مرة أخرى باستخدام
+
+108
+00:08:10,990 --> 00:08:17,430
+العدسات العينية، تمام؟ فبالتالي، إذاً لحتى الآن نحن
+
+109
+00:08:17,430 --> 00:08:20,810
+نعرف أن الـ course adjustment هو الذي مسؤول عن
+
+110
+00:08:20,810 --> 00:08:25,410
+تقريب الـ stage من العدسات الشيئية، أو تقريب العدسات
+
+111
+00:08:25,410 --> 00:08:29,050
+الشيئية من الـ stage، ليتم التقاط الصورة الأولية،
+
+112
+00:08:29,050 --> 00:08:32,730
+أنه يعني العدسات الشيئية تكبر لي الـ.. الـ.. الـ
+
+113
+00:08:32,730 --> 00:08:37,730
+objects، تمام؟ أو الـ.. التي هي محتويات العينة. طيب،
+
+114
+00:08:37,730 --> 00:08:42,970
+الـ fine adjustment، هذا طيب، لإيش وظيفته؟ كل خلية،
+
+115
+00:08:42,970 --> 00:08:48,630
+كل كائن حي، كل molecule، كل مكون object،
+
+116
+00:08:48,630 --> 00:08:53,550
+عنصر نحن نفحصه، يكون له أبعاد، هو ما يكون مصمّم
+
+117
+00:08:53,550 --> 00:08:56,110
+يعني هكذا نايم على الـ slide، وما.. لأ، يكون له
+
+118
+00:08:56,110 --> 00:09:02,190
+طول، عرض، ارتفاع، تمام؟ فالـ.. الـ.. الـ fine
+
+119
+00:09:02,190 --> 00:09:06,760
+adjustment، الـ fine adjustment، تمام؟ هو عبارة عن
+
+120
+00:09:06,760 --> 00:09:11,960
+العجل الذي يتحكم أيضاً بـ.. بقرب العدسات الشيئية
+
+121
+00:09:11,960 --> 00:09:16,660
+من الـ.. الـ.. الـ object، ولكن كيف يتم.. يعني أو
+
+122
+00:09:16,660 --> 00:09:24,750
+كيف يتم تحكمه؟ هو يتحكم بعمق الصورة، يتحكم بعمق
+
+123
+00:09:24,750 --> 00:09:28,150
+الصورة، يعني ممكن يعرض لي تفاصيل مثلاً هنا، أقل أجبت،
+
+124
+00:09:28,150 --> 00:09:32,070
+خلية، بدي أشوفها خلية animal cell، خلية حيوانية،
+
+125
+00:09:32,070 --> 00:09:36,730
+تمام؟ هذه الخلية الحيوانية في في.. هي مش.. يعني
+
+126
+00:09:36,730 --> 00:09:41,850
+إيه؟ هي لها أبعاد، ولها طول، عرض، ارتفاع، تمام؟ طيب،
+
+127
+00:09:41,850 --> 00:09:45,030
+وهذه الخلية في داخلها، في عندي مثلاً endoplasmic
+
+128
+00:09:45,030 --> 00:09:48,860
+reticulum، شبكة إندوبلازمية، فيها golgi apparatus،
+
+129
+00:09:48,860 --> 00:09:52,420
+جهاز جولجي، فيها نواة، فيها ريبوسومات، فيها
+
+130
+00:09:52,420 --> 00:09:56,760
+سيتوبلازم، تمام؟ الآن، لو أنا أشوفها أول مرة، لما
+
+131
+00:09:56,760 --> 00:10:00,680
+أحطها على الـ slide، وأقرب باستخدام الـ course
+
+132
+00:10:00,680 --> 00:10:04,380
+adjustment، أقرب الـ.. الـ stage من العدسة الشيئية،
+
+133
+00:10:04,380 --> 00:10:08,700
+وأشوفها، ممكن ما أشوف كل تفاصيل الصورة، يعني ممكن
+
+134
+00:10:08,700 --> 00:10:12,600
+بس تظهر لي الـ membrane تبع الخلية، وتظهر لي النواة
+
+135
+00:10:12,600 --> 00:10:19,110
+فقط، لا غير، تمام؟ تظهر لي نواة الخلية فقط. طيب، لو
+
+136
+00:10:19,110 --> 00:10:22,670
+أنا بدي أشوف تفاصيل أكثر، وهي مش موجودة.. يعني
+
+137
+00:10:22,670 --> 00:10:25,070
+هذه التفاصيل، ممكن يكون golgi مش موجودة على نفس
+
+138
+00:10:25,070 --> 00:10:29,350
+العمق تبع الـ.. الـ.. النواة، فـ.. أو الـ endoplasmic
+
+139
+00:10:29,350 --> 00:10:33,490
+reticulum، مثلاً مش موجودة على عمق golgi، تمام؟ لأنه
+
+140
+00:10:33,490 --> 00:10:38,160
+نحن حكينا الخلية لها أبعاد. فالـ.. الـ fine
+
+141
+00:10:38,160 --> 00:10:43,320
+adjustment هو الذي يتحكم بعمق الصورة، يعني ممكن
+
+142
+00:10:43,320 --> 00:10:46,640
+إذا كان أنا الصورة الأولية كان فقط مبين فيها
+
+143
+00:10:46,640 --> 00:10:51,140
+membrane ونواة، الـ fine adjustment، لما أحركه، تمام،
+
+144
+00:10:51,140 --> 00:10:56,110
+لما ألعب فيه، ممكن يبين لي golgi، يختفي golgi، يتبين
+
+145
+00:10:56,110 --> 00:10:59,670
+endoplasmic reticulum. إذاً هو يبحث على أبعاد أو
+
+146
+00:10:59,670 --> 00:11:04,310
+على أعماق مختلفة من الصورة ذات نفسها، تمام؟ ليش؟
+
+147
+00:11:04,310 --> 00:11:08,230
+لأن مكونات الـ object المراد فحصه أحياناً تكون
+
+148
+00:11:08,230 --> 00:11:11,290
+شكلها على نفس المسافة، لأن الـ object هذا له
+
+149
+00:11:11,290 --> 00:11:15,970
+أبعاد، تمام؟ له أبعاد. فإذاً الـ fine adjustment
+
+150
+00:11:15,970 --> 00:11:20,910
+يُوريني الـ details، تمام، يُوريني الـ details. هذا
+
+151
+00:11:20,910 --> 00:11:25,430
+الـ fine adjustment، أستخدمه خصوصاً لو كنت أنا أفحص
+
+152
+00:11:25,430 --> 00:11:30,970
+العينة على الـ high power objective lens، على الـ..
+
+153
+00:11:30,970 --> 00:11:36,770
+العدسات الشيئية التي قوة تكبيرها كبيرة، نوعاً عاماً.
+
+154
+00:11:36,770 --> 00:11:41,710
+طيب، Condenser Adjustment، فيه عندي أيضاً ناب قضيب أو
+
+155
+00:11:41,710 --> 00:11:46,110
+عجل هنا، هذا نسميه Condenser Adjustment. إيش وظيفة
+
+156
+00:11:46,110 --> 00:11:49,790
+الـ Condenser Adjustment؟ هو يتحكم، هـ.. this moves
+
+157
+00:11:49,790 --> 00:11:53,850
+the condenser up or down. يحرك الـ condenser إلى
+
+158
+00:11:53,850 --> 00:11:58,490
+أعلى وإلى أسفل، تمام؟ يحركه إلى أعلى وإلى أسفل.
+
+159
+00:11:59,380 --> 00:12:03,900
+فإذاً خلّينا بس نرجع نشوف كيف يتحرك. هذا هو الـ
+
+160
+00:12:03,900 --> 00:12:06,920
+condenser الذي هنا، الذي هنا نسميه condenser، تحت
+
+161
+00:12:06,920 --> 00:12:10,580
+الـ stage مباشرة، وهي هذه عدسته التي مبينة من فتحة
+
+162
+00:12:10,580 --> 00:12:15,560
+الـ stage. طيب، إيش وظيفة الـ condenser؟ هو يأخذ
+
+163
+00:12:15,560 --> 00:12:18,000
+الضوء من الـ light source، هنا في عندي light source،
+
+164
+00:12:18,000 --> 00:12:22,220
+في عندي لمبة تعطيني ضوءاً، يستلم الضوء، ومن اسمه
+
+165
+00:12:22,220 --> 00:12:25,400
+condenser، يكثّف الضوء، يجمعه، علشان الذي جاء الضوء
+
+166
+00:12:25,400 --> 00:12:29,400
+قادر يخترق العينة ويصل إلى العدسة الشيئية، فهنا
+
+167
+00:12:29,400 --> 00:12:34,980
+يتم وصول صورة واضحة جداً إلى العدسة الشيئية، تمام؟
+
+168
+00:12:35,610 --> 00:12:42,390
+لأن الضوء تم تكثيفه واختراقه في الـ.. في العينة.
+
+169
+00:12:42,390 --> 00:12:47,050
+الآن نحن مثلاً، إيش مبدأ الرؤية طبعاً؟ الأشياء..
+
+170
+00:12:47,050 --> 00:12:50,510
+الضوء يسقط على الأشياء، ينعكس إلى.. إلى عيوننا،
+
+171
+00:12:50,510 --> 00:12:55,310
+فنرى الأشياء، صح؟ بدليل أنه بالعتمة نحن ما نشوف،
+
+172
+00:12:55,990 --> 00:13:00,230
+لأنه ما في ضوء يسقط على الأشياء وينعكس على عيوننا،
+
+173
+00:13:00,230 --> 00:13:
+
+223
+00:16:56,830 --> 00:17:00,950
+The principal purpose of the condenser is to condense
+
+224
+00:17:00,950 --> 00:17:04,690
+the light. وظيفة الـ condenser أنه يكسر الضوء
+
+225
+00:17:04,690 --> 00:17:07,870
+الذي... والذي يخترق العينة، والذي نحن
+
+226
+00:17:07,870 --> 00:17:12,430
+نحتاجه للـ visualization للإضاءة والـ... والـ
+
+227
+00:17:12,430 --> 00:17:18,260
+...والتكبير، وأنه يلتقط صورة واضحة. طيب، حكينا
+
+228
+00:17:18,260 --> 00:17:22,920
+لحتى الآن عن الـ frame، الهيكل للميكروسكوب، حكينا
+
+229
+00:17:22,920 --> 00:17:27,600
+عن جهاز التركيز أو نظام التركيز، الذي هو عبارة عن
+
+230
+00:17:27,600 --> 00:17:31,580
+course adjustment, fine adjustment, condenser
+
+231
+00:17:31,580 --> 00:17:38,590
+adjustment، والـ iris diaphragm، وأيضًا راح نحكي عن
+
+232
+00:17:38,590 --> 00:17:41,770
+نظام التكبير، طبعًا؟ خلّيني أحكي... حكينا عن نظام
+
+233
+00:17:41,770 --> 00:17:46,230
+التركيز، هنحكي عن نظام التكبير، طبعًا؟ ما هي
+
+234
+00:17:46,230 --> 00:17:50,730
+الأدوات التي مسؤولة عن تكبير الـ object في الـ
+
+235
+00:17:50,730 --> 00:17:56,150
+microscope؟ هي الـ objective lenses، طبعًا؟ والـ
+
+236
+00:17:56,150 --> 00:17:59,130
+ocular lenses، إذن العدسات التي موجودة في الـ
+
+237
+00:17:59,130 --> 00:18:04,480
+microscope هي التي مسؤولة عن التكبير. في عندي عدسات
+
+238
+00:18:04,480 --> 00:18:09,000
+objective lenses، تمام؟ ومن اسمها objective، ليش
+
+239
+00:18:09,000 --> 00:18:12,120
+تسمى objective؟ لأنها قريبة من الـ object المراد
+
+240
+00:18:12,120 --> 00:18:17,700
+فحصه، تمام؟ قريبة من الـ object المراد فحصه، لذلك تسمى
+
+241
+00:18:17,700 --> 00:18:23,760
+objective. طيب هذه العدسات أربعة، كل عدسة لها قوة
+
+242
+00:18:23,760 --> 00:18:29,160
+تكبير مختلفة، هي أنا عندي 4x، يعني الـ... الـ object
+
+243
+00:18:29,160 --> 00:18:34,760
+بيكون مكبر أربع مرات، تمام؟ الـ... في... في الصورة
+
+244
+00:18:34,760 --> 00:18:38,100
+الأولية التي يتم التقاطها.
+
+245
+00:18:39,060 --> 00:18:44,300
+تمام؟ هنا الـ object يتم تكبيره 10x، يعني عشر مرات
+
+246
+00:18:44,300 --> 00:18:49,180
+في الصورة الأولية، هنا عندي 40x وهنا 100x، تمام؟
+
+247
+00:18:49,180 --> 00:18:54,300
+لما أحكيلك low power objective، تمام؟ يعني قوة
+
+248
+00:18:54,300 --> 00:18:56,980
+تكبير قليلة من الـ objective، معناها بدك تستخدم
+
+249
+00:18:56,980 --> 00:19:01,210
+العدسة أربعة أو عشرة. لمّا أحكيلك high power
+
+250
+00:19:01,210 --> 00:19:05,130
+objective، يعني بدك تستخدم العدسة أربعين، لما
+
+251
+00:19:05,130 --> 00:19:09,190
+أحكيلك oil immersion objective، يعني العدسة مئة،
+
+252
+00:19:09,190 --> 00:19:13,670
+تمام؟ إذًا إما بنعرفهم بقوة التكبير تبعتهم أو
+
+253
+00:19:13,670 --> 00:19:18,370
+بـ power تبعهم، هذه low power, high power, oil
+
+254
+00:19:18,370 --> 00:19:18,850
+immersion.
+
+255
+00:19:21,980 --> 00:19:27,360
+طيب، إذًا، والعدسات العينية هي الـ upper optical
+
+256
+00:19:27,360 --> 00:19:30,260
+component، التي هي الجزء الذي... الذي نحن نشوف
+
+257
+00:19:30,260 --> 00:19:35,520
+فيه، ويكون موجود في أعلى الـ microscope، وطبعًا
+
+258
+00:19:35,520 --> 00:19:39,960
+وظيفته، حكينا، أنه العدسات الشيئية تلتقط صورة أولية
+
+259
+00:19:39,960 --> 00:19:46,880
+مكبرة، إما 4X أو 10X أو 40X أو 100X، تمام؟ هذه الصورة
+
+260
+00:19:46,880 --> 00:19:54,070
+الأولية، تمام؟ تنتقل عبر الـ tube إلى الـ... الـ... الـ
+
+261
+00:19:54,070 --> 00:19:58,190
+ocular lenses، فهي الـ ocular lenses، وظيفتها أنها
+
+262
+00:19:58,190 --> 00:20:03,030
+magnify the primary image، تكبر الصورة الأولية مرة
+
+263
+00:20:03,030 --> 00:20:06,810
+أخرى، تمام؟ وهذه العدسات موجودة، ممكن يكون قوة
+
+264
+00:20:06,810 --> 00:20:13,290
+تكبيرها 4x, 6x, 7x أو 10 أو حتى 15 أو 20x، تمام؟
+
+265
+00:20:13,290 --> 00:20:16,630
+فإذًا لها قوات تكبير مختلفة، الـ more common use
+
+266
+00:20:16,630 --> 00:20:20,150
+الذي أنتم ستستخدموه في الميكروسكوبات، الـ ocular lenses
+
+267
+00:20:20,150 --> 00:20:26,760
+تبعها 10x، تمام؟ ف... إذًا هي تكبر الصورة
+
+268
+00:20:26,760 --> 00:20:31,840
+المبدئية أو الأولية التي وصلتها من الـ objective في
+
+269
+00:20:31,840 --> 00:20:41,160
+عندي microscope، والـ... طبعًا الـ... بنوعين حسب عدد
+
+270
+00:20:41,160 --> 00:20:44,800
+الـ eyepiece الذي موجود فيه في عندي الـ microscope
+
+271
+00:20:44,800 --> 00:20:48,960
+الذي يكون فيه one ocular lenses أو one eyepiece،
+
+272
+00:20:49,460 --> 00:20:57,700
+نسميه mono-ocular، طبعًا، إذا كان في عدستين عينيتين، two
+
+273
+00:20:57,700 --> 00:21:02,880
+أو ocular lenses، نسميه bi-ocular، طبعًا، إذا الـ
+
+274
+00:21:02,880 --> 00:21:06,340
+microscope نسميه bi-ocular أو mono-ocular حسب عدد
+
+275
+00:21:06,340 --> 00:21:10,600
+العدسات العينية التي موجودة فيه. جدير بالذكر أن
+
+276
+00:21:10,600 --> 00:21:14,360
+أحكي eyepiece أو أحكي ocular lenses، نفس المعنى.
+
+277
+00:21:16,460 --> 00:21:21,920
+طيب، حكينا عن جهاز أو نظام التكبير، نحكي عن نظام الـ
+
+278
+00:21:21,920 --> 00:21:26,500
+illumination، الإضاءة، والتحكم بالإضاءة، أيضًا الـ
+
+279
+00:21:26,500 --> 00:21:32,720
+condenser والـ iris، من الـ... يتبعوا للنظام التوضيح
+
+280
+00:21:32,720 --> 00:21:36,650
+و الإضاءة، تمام؟ الذي هو، احنا حكينا الـ Condenser
+
+281
+00:21:36,650 --> 00:21:40,330
+وظيفته أنه بي... بيجمع الضوء الذي واصل من الـ
+
+282
+00:21:40,330 --> 00:21:43,550
+light source، تمام؟ ويخليه يخترق العينة، التي
+
+283
+00:21:43,550 --> 00:21:47,190
+يمكن رؤية الصورة الأولية بوسط الـ objective lens
+
+284
+00:21:47,190 --> 00:21:52,070
+تمام؟ وده هي الـ... the condenser، عدسة الـ condenser
+
+285
+00:21:52,070 --> 00:21:55,710
+هاي التي تكون... حكينا، تكون مبينة من فتحة الـ...
+
+286
+00:21:55,710 --> 00:21:59,150
+الـ... الـ stage، بتكون تستلم الضوء من وين؟ من الـ
+
+287
+00:21:59,150 --> 00:22:02,850
+light source، وبتمرره خلال العينة وإلى الـ
+
+288
+00:22:02,850 --> 00:22:05,890
+objective. هذا الـ iris، ده في الرقم شايفينه، بيكون
+
+289
+00:22:05,890 --> 00:22:07,230
+موجود تحت الـ condenser.
+
+290
+00:22:12,640 --> 00:22:19,000
+طبعًا، مع حركة القرص هذا، زي حجاب بيسكر وبيفتح، طبعًا
+
+291
+00:22:19,000 --> 00:22:22,720
+بيسكر وبيفتح، إذًا بتحكم بكمية الضوء التي مارة من
+
+292
+00:22:22,720 --> 00:22:26,140
+الـ light source إلى الـ condenser. هين زي ما أنتم
+
+293
+00:22:26,140 --> 00:22:29,460
+شايفين، هي هذا القرص مفتوح على الآخر، هذا مغلق
+
+294
+00:22:29,460 --> 00:22:33,580
+شوية، هذا مغلق أكثر، فإذًا حسب حركة الـ... الـ...
+
+295
+00:22:33,580 --> 00:22:37,480
+القرص diaphragm هذا بي... بي... بيسكرلي الـ
+
+296
+00:22:37,480 --> 00:22:42,130
+diaphragm أو بيفتحه، تمام؟ إذًا the iris is
+
+297
+00:22:42,130 --> 00:22:46,230
+a mechanical device mounted underneath the condenser
+
+298
+00:22:46,230 --> 00:22:50,930
+موجودة تحت الـ condenser، and controls the amount of
+
+299
+00:22:50,930 --> 00:22:55,470
+light، بتتحكم بكمية الضوء التي داخلة على الـ condenser
+
+300
+00:22:55,470 --> 00:22:58,730
+من وين؟ من الـ light source، تمام؟ من الـ light
+
+301
+00:22:58,730 --> 00:23:03,710
+source. يوجد أيضًا، أحيانًا بيكون mirror، وين بتكون الـ
+
+302
+00:23:03,710 --> 00:23:08,020
+mirror هذه؟ نرجع لصورة الـ microscope، بيكون هنا
+
+303
+00:23:08,020 --> 00:23:12,040
+أحيانًا فوق الـ light source، بيكون فيه مراية، ليش؟
+
+304
+00:23:12,040 --> 00:23:15,180
+المراية هذه وظيفتها أنها تأخذ الضوء الذي جاي من الـ
+
+305
+00:23:15,180 --> 00:23:21,000
+light source وتعكسه لحتى يتم انعكاسه إلى الـ
+
+306
+00:23:21,000 --> 00:23:25,020
+condenser، تمام؟ ليعكس إلى الـ condenser، بس يعني
+
+307
+00:23:25,020 --> 00:23:32,720
+لتعزيز كفاءة استخدام الضوء فقط لا غير. أحيانًا في...
+
+308
+00:23:34,540 --> 00:23:37,880
+ميكروسكوبات ما بتعمل على... يعني ما فيها لمبة،
+
+309
+00:23:37,880 --> 00:23:41,100
+بتعمل على ضوء النهار أو ضوء المنطقة المحيطة بها،
+
+310
+00:23:41,100 --> 00:23:44,720
+فلزام يكون في عندي عدسة جامع للضوء، لحتى
+
+311
+00:23:44,720 --> 00:23:47,360
+تجمع الضوء الذي موجود في المنطقة التي موجودة فيها
+
+312
+00:23:47,360 --> 00:23:51,880
+المايكروسكوب، وتوجهه باتجاه الـ Condenser، تمام؟
+
+313
+00:23:51,880 --> 00:23:57,120
+فإذًا هذه فائدة الـ mirror، الـ mirror بتكون تحت الـ
+
+314
+00:23:57,120 --> 00:24:02,260
+condenser، وتحت القرص أيضًا، وفوق الـ light source
+
+315
+00:24:02,260 --> 00:24:06,440
+مباشرة، وإيش وظيفتها؟ reflect the beam of light،
+
+316
+00:24:06,440 --> 00:24:10,560
+تعكس، اللي هو الضوء إلى... باتجاه الـ condenser، into
+
+317
+00:24:10,560 --> 00:24:16,580
+the condenser. ماشي؟ وسواء كان هذا الضوء جاي من الـ
+
+318
+00:24:16,580 --> 00:24:19,520
+daylight أو من الـ electrical light، من الضوء
+
+319
+00:24:19,520 --> 00:24:21,860
+الكهربائي أو اللمبة التي موجودة في الـ microscope.
+
+320
+00:24:23,750 --> 00:24:28,950
+طيب، إذًا مصادر الإضاءة، daylight، الذي هو يعني ضوء
+
+321
+00:24:28,950 --> 00:24:34,330
+المحيط بالجهاز، يعني أن أكتفي بالضوء النهار مثلًا، و
+
+322
+00:24:34,330 --> 00:24:40,110
+هذا ممكن يكون sufficient، يعني كافي في بعض الأشياء،
+
+323
+00:24:40,110 --> 00:24:44,750
+ولكن أحيانًا بيكون غير كافي، إذا أنا بدأت أستخدم قوة
+
+324
+00:24:44,750 --> 00:24:47,650
+تكبير كبيرة زي الـ oil immersion، التي هي الـ 100x.
+
+325
+00:24:47,950 --> 00:24:52,430
+حكينا إنه مع قوة التكبير القليلة، تمام؟ الـ
+
+326
+00:24:52,430 --> 00:24:56,070
+objective lenses ما بتكون محتاجة ضوء كثير، لكن مع
+
+327
+00:24:56,070 --> 00:24:59,630
+الـ... الـ... القوة التكبير العالية، الـ objective
+
+328
+00:24:59,630 --> 00:25:05,190
+بتكون محتاجة كمية ضوء أكبر، تمام؟ بتكون محتاجة
+
+329
+00:25:05,190 --> 00:25:12,510
+كمية ضوء أكبر، الـ electric light، تمام؟ الذي هو الـ
+
+330
+00:25:12,510 --> 00:25:16,830
+...الـ... الـ مصدر الضوء، اللمبة، بتكون tungsten lamp،
+
+331
+00:25:16,830 --> 00:25:20,810
+تمام؟ بتكون tungsten lamp، أحيانًا ممكن تكون طبعًا
+
+332
+00:25:20,810 --> 00:25:25,230
+tungsten lamp أو quartz halogen lamp، حسب يعني نوع
+
+333
+00:25:25,230 --> 00:25:28,850
+الـ microscope، وحسب نوع الإضاءة... نوع الإضاءة
+
+334
+00:25:28,850 --> 00:25:34,050
+التي موجودة أو التي نحن نحتاجها، طبعًا، في الـ
+
+335
+00:25:34,050 --> 00:25:39,590
+microscope. طيب، فلاتر، أحيانًا بيكون أيضًا موجود في الـ
+
+336
+00:25:39,590 --> 00:25:43,990
+...الـ... الـ... الـ illumination system، فلاتر.
+
+337
+00:25:47,230 --> 00:25:50,630
+طيب، الفلتر هذا عبارة عن إيش؟ هو عبارة عن قطعة
+
+338
+00:25:50,630 --> 00:25:55,790
+زجاجية ملونة، تمام؟ وإيش وظيفته؟ يقلل شدة الضوء
+
+339
+00:25:55,790 --> 00:25:58,970
+الذي مع الـ... دو كمان، بيكون موجود الفلتر
+
+340
+00:25:58,970 --> 00:26:04,910
+موجود، يعني، ممكن أحطه هنا، هو بـ... طبعًا هو الذي
+
+341
+00:26:04,910 --> 00:26:09,530
+بتحكي، يعني ممكن أنا أركبه أو أشيله، تمام؟ يعني أنا
+
+342
+00:26:09,530 --> 00:26:13,110
+بتحكم بإن هو موجود أو غير موجود، بس هو بيجي مع الـ
+
+343
+00:26:13,110 --> 00:26:17,370
+microscope، فلاتر ضوئية، تمام؟ قطعة زجاجية ملونة، تكون
+
+344
+00:26:17,370 --> 00:26:22,170
+موجودة على الـ... الـ light source، أنا بأحطها على الـ
+
+345
+00:26:22,170 --> 00:26:25,250
+light source هنا، عشان تتحكم بكمية الضوء التي
+
+346
+00:26:25,250 --> 00:26:29,650
+موجودة، ولعل هذه الفكرة طرحناها في محاضرة الـ
+
+347
+00:26:29,650 --> 00:26:34,510
+calorie meter، وذكرنا أنه لو كان في عندي لمبة وأنا
+
+348
+00:26:34,510 --> 00:26:39,850
+حطيت زجاج ملون، هذا الزجاج... الزجاج راح يحجب كمية
+
+349
+00:26:39,850 --> 00:26:46,010
+من الضوء، ويمرر ما تبقى، تمام؟ إذًا هو بيتحكم بكمية
+
+350
+00:26:46,010 --> 00:26:48,830
+الضوء التي موجودة أو التي... التي تعبر إلى
+
+351
+00:26:48,830 --> 00:26:52,850
+الـ condenser ومن ثم إلى الـ objective. طيب، ممكن كمان
+
+352
+00:26:52,850 --> 00:26:56,270
+أحيانًا في بعض الأجزاء، خصوصًا الأجزاء التي غير
+
+353
+00:26:56,270 --> 00:27:02,310
+مسبوغة، ممكن يزيد الإيضاح، increase resolution، تمام؟
+
+354
+00:27:02,310 --> 00:27:06,370
+لأنه في بعض الأشياء التي نحن نكون محتاجين نشوفها
+
+355
+00:27:06,530 --> 00:27:12,830
+بتكون هي معتمة، تمام؟ فمش محتاجة ضوء كثير يخليها
+
+356
+00:27:12,830 --> 00:27:17,890
+فاتحة وغير مرئية. طيب، وأيضًا كمان وظيفة الـ filter
+
+357
+00:27:17,890 --> 00:27:21,190
+أنّه protect the eye from injury، هلأ احنا مع
+
+358
+00:27:21,190 --> 00:27:28,650
+الاستخدام المطول للـ Microscope، يتم... بيصير يعني
+
+359
+00:27:28,650 --> 00:27:32,030
+لمعة الضوء في عيننا مؤذية، فممكن نستخدم الـ filter
+
+360
+00:27:32,030 --> 00:27:35,050
+عشان نحد من كمية الضوء التي يعني بيخبط في عينينا،
+
+361
+00:27:35,050 --> 00:27:40,770
+فبالتالي، أو أنه يعني نحافظ على سلامة العين أو نكون
+
+362
+00:27:40,770 --> 00:27:45,930
+مرتاحين أكثر وأحنا بنشتغل. طيب، هيك بنكون عرضنا
+
+363
+00:27:45,930 --> 00:27:53,240
+مكونات الـ... الـ... الـ microscope، سردناها كسرد أولي،
+
+364
+00:27:53,240 --> 00:27:57,740
+وبعد هيك حكينا إنها مكونة من أجزاء، الجزء الأول هو
+
+365
+00:27:57,740 --> 00:28:03,260
+الهيكل، ثلاثة... ثلاثة مناطق، منطقة الـ arm والـ stage، و
+
+366
+00:28:03,260 --> 00:28:07,820
+منطقة الـ base. الجزء الثاني هو منطقة، اللي هو الـ
+
+367
+00:28:07,820 --> 00:28:10,900
+focusing system، الذي هو عبارة عن الـ course والـ
+
+368
+00:28:10,900 --> 00:28:14,220
+fine adjustment، وأيضًا الـ condenser adjustment، و
+
+369
+00:28:14,220 --> 00:28:18,000
+ممكن ييجي معهم القرص. حكينا بعدين عن الـ elimination
+
+370
+00:28:18,000 --> 00:28:23,560
+system، الذي هو الإضاءة، الـ light source، وأيضًا كمان
+
+371
+00:28:23,560 --> 00:28:27,400
+الـ condenser والقرص، ممكن يكونوا جزء من الـ light
+
+372
+00:28:27,400 --> 00:28:32,040
+source أو من الـ illumination system، والـ filter، و
+
+373
+00:28:32,040 --> 00:28:36,360
+الـ mirror. حكينا عن الـ magnification system، نظام
+
+374
+00:28:36,360 --> 00:28:40,740
+التكبير، الذي هو يشمل الـ objective lenses والـ
+
+375
+00:28:40,740 --> 00:28:46,070
+ocular lenses. طبعًا حتى كمان شدة الضوء يا بنات، ممكن
+
+376
+00:28:46,070 --> 00:28:50,830
+أنا أتحكم فيها من هنا، في عندي عجل بيزيد الضوء لـ...
+
+377
+00:28:50,830 --> 00:28:56,110
+الضوء، اللمبة نفسها، بيزيد... بيزيدها وبيقلل، طبعًا،
+
+378
+00:28:56,110 --> 00:29:00,610
+وفي... في كبسة أيضًا هنا موجودة، بتفتح وبتسكر
+
+379
+00:29:00,610 --> 00:29:10,190
+الضوء. طيب، هذا بالنسبة لـ... مكونات. خلينا نشوف مبدأ
+
+380
+00:29:10,190 --> 00:29:13,770
+عمل الـ oil immersion، ليش إحنا بنحكي عن العدسة التي
+
+381
+00:29:13,770 --> 00:29:22,010
+قوة تكبيرها 100X، تمام؟ ليش بنسميها oil immersion؟
+
+382
+00:29:22,010 --> 00:29:26,670
+تمام. طيب، قبل أن نحكي عن الـ oil immersion، خلينا
+
+383
+00:29:26,670 --> 00:29:31,100
+نحكي عن الـ microscope بشكل عام، إيش مبدأ عمله؟ الـ...
+
+384
+00:29:31,100 --> 00:29:35,940
+الـ microscope بشكل عام هو magnifying instrument،
+
+385
+00:29:35,940 --> 00:29:42,500
+هو عبارة عن جهاز تكبير، تمام؟ والصورة التي تصل
+
+386
+00:29:42,500 --> 00:29:46,400
+الـ... الـ... طبعًا الـ... الـ... لأ، الـ objective
+
+387
+00:29:46,400 --> 00:29:51,320
+lenses بتكون هي عبارة عن الـ primary، تمام؟ image.
+
+388
+00:29:52,800 --> 00:29:56,920
+بأول حاجة، الضوء يخترق الـ object التي موجودة على
+
+389
+00:29:56,920 --> 00:30:00,760
+الـ stage، تمام؟ ويصل إلى الـ objective، فالـ
+
+390
+00:30:00,760 --> 00:30:05,040
+objective تجمع الضوء هذا، وتلتقط صورة، الصورة هاي
+
+391
+00:30:05,040 --> 00:30:09,420
+عبارة عن الـ primary image، مكبرة بقوة تكبير الـ
+
+392
+00
+
+445
+00:34:17,770 --> 00:34:22,330
+بحافة العدسة، هذه الزاوية بنسميها الـmu أو هذه
+
+446
+00:34:22,330 --> 00:34:25,450
+الـmu، واحدة منهم، طبعا؟ فهذه
+
+447
+00:34:27,650 --> 00:34:32,750
+الزاوية assigned  للميو  تستخدم في حساب الـ numerical
+
+448
+00:34:32,750 --> 00:34:36,290
+aperture. طيب بيحكي لي هنا الـ numerical aperture
+
+449
+00:34:37,410 --> 00:34:41,510
+depends on the radius of the lens. تمام؟ هذا الـ
+
+450
+00:34:41,510 --> 00:34:48,370
+numerical aperture يعتمد على قطر العدسة. اه والله
+
+451
+00:34:48,370 --> 00:34:55,010
+كل ما زاد قطر العدسة، تمام؟ شايفين؟ كل ما زاد قطر
+
+452
+00:34:55,010 --> 00:35:02,520
+العدسة، معناها أن الزاوية الجديدة هذه
+
+453
+00:35:02,520 --> 00:35:06,940
+أكبر، تمام؟ ومحتاجة ضوء أكثر. هي دي، شايفين كمية
+
+454
+00:35:06,940 --> 00:35:10,020
+الضوء اللي داخلة أكثر، فبالتالي الـ numerical
+
+455
+00:35:10,020 --> 00:35:14,280
+aperture يعتمد على قطر العدسة. هي هنا، مثلاً، مثلاً
+
+456
+00:35:14,280 --> 00:35:19,840
+شايفين؟ numerical aperture 95%، كمية الضوء كبيرة.
+
+457
+00:35:19,840 --> 00:35:24,700
+قلّت الـ numerical aperture، قلت كمية الضوء، فقلت
+
+458
+00:35:24,700 --> 00:35:27,640
+وأكثر، فأكثر. نتيجة أن الـ numerical aperture قليلة.
+
+459
+00:35:27,640 --> 00:35:30,480
+إذا هو بيعبر لي عن إيش؟ كمية الضوء اللي بده تروح على
+
+460
+00:35:30,480 --> 00:35:36,080
+الـ objective lens، على العدسة الشيئية، تمام؟ إذا
+
+461
+00:35:36,080 --> 00:35:39,240
+بنحسبه بالـ R، refractive index of glass، حكينا لأن
+
+462
+00:35:39,240 --> 00:35:42,780
+العدسة مصنوعة من الـ glass، وأيضاً الـ mu، اللي هي الزاوية
+
+463
+00:35:42,780 --> 00:35:47,220
+الـ angle، الزاوية المصنوعة made by، مصنوعة من ray،
+
+464
+00:35:47,220 --> 00:35:53,690
+شعاع يمر بالحافة العدسة، the edge of lens، والآخر
+
+465
+00:35:53,690 --> 00:35:59,110
+يمر بـ the center of the lens، مركز العدسة، تمام؟
+
+466
+00:35:59,110 --> 00:36:06,230
+resolving power، قوة الإضاح، تمام؟ وطيب، جدير بالذكر
+
+467
+00:36:06,230 --> 00:36:10,970
+أو يعني حتى أذكر، إحنا حكينا معلومة مهمة أن
+
+468
+00:36:10,970 --> 00:36:19,030
+العدسات اللي قوة تكبيرها كبيرة، تمام؟ محتاجة أن ضوء
+
+469
+00:36:19,030 --> 00:36:23,130
+أكثر، فمحتاجة أن أنا، مثلاً، أفتح القرص عشان أتمكن أن
+
+470
+00:36:23,130 --> 00:36:29,250
+أن الضوء يصل الـ condenser أكثر. أما العدسات اللي
+
+471
+00:36:29,250 --> 00:36:34,930
+قوة تكبيرها قليلة، زي الـ low power lens، تمام؟ هي
+
+472
+00:36:34,930 --> 00:36:40,510
+بتكون مش محتاجة ضوء كثير، فبالتالي ممكن أنا أسكر
+
+473
+00:36:40,510 --> 00:36:43,910
+القرص عشان كمية الضوء اللي داخلة للعدسة تكون قليلة.
+
+474
+00:36:47,590 --> 00:36:53,950
+resolving power، اللي هي قوة التكبير، تمام؟ هي يعني
+
+475
+00:36:53,950 --> 00:36:56,430
+التعبير عن الـ magnification، بنعبر عن الـ
+
+476
+00:36:56,430 --> 00:37:00,070
+magnification باستخدام الـ resolving power، بنعبر
+
+477
+00:37:00,070 --> 00:37:01,770
+عن التكبير بقوة الإضاءة.
+
+478
+00:37:04,950 --> 00:37:09,230
+يُعرف هذا الـ resolving power بأنه هو the ability of
+
+479
+00:37:09,230 --> 00:37:12,750
+microscope، قدرة الميكروسكوب، at the specific
+
+480
+00:37:12,750 --> 00:37:17,210
+magnification، على قوة تكبير معينة، to distinguish
+
+481
+00:37:17,210 --> 00:37:22,590
+بين أنه يميز two separate objects situated close to
+
+482
+00:37:22,590 --> 00:37:26,410
+one another، أنه يميز ما بين نقطتين أو two objects
+
+483
+00:37:26,410 --> 00:37:29,850
+أو عنصرين موجودين في الشريحة أو في العينة المراد
+
+484
+00:37:29,850 --> 00:37:34,150
+فحصها ويكونوا قراب من بعضهم البعض. كل ما كانت
+
+485
+00:37:34,150 --> 00:37:37,070
+المسافة ما بين هذين الـ two objects، هذين النقطتين
+
+486
+00:37:37,070 --> 00:37:41,310
+أو العنصرين، كل ما كانت المسافة ما بينهم قليلة، the
+
+487
+00:37:41,310 --> 00:37:46,790
+smaller the distance، تمام؟ the smaller the
+
+488
+00:37:46,790 --> 00:37:50,850
+distance، هيكون أن كل ما
+
+489
+00:37:50,850 --> 00:37:55,530
+كانت المسافة ما بينهم صغيرة، وتمكن الـ microscope من
+
+490
+00:37:55,530 --> 00:37:59,930
+إيضاحهم وتكبيرهم. إذا والله هذا الـ microscope عنده
+
+491
+00:37:59,930 --> 00:38:05,310
+greater resolving power، تمام؟ عنده قوة إيضاح أكبر.
+
+492
+00:38:05,310 --> 00:38:12,770
+طالما أنه بيفصل لي، يعني تفاصيل، وبيوضح لي، يعني نقاط
+
+493
+00:38:12,770 --> 00:38:16,750
+أو عناصر أو أشياء قريبة جداً من بعضهم البعض، المسافة
+
+494
+00:38:16,750 --> 00:38:20,470
+ما بينهم قليلة جداً، كانت المسافة ما بينهم قليلة
+
+495
+00:38:20,470 --> 00:38:25,830
+كثيراً، وهو تمكن من إيضاحهم، إذا هو عنده greater
+
+496
+00:38:25,830 --> 00:38:31,070
+resolving power، تمام؟ طيب، الـ minimum distance،
+
+497
+00:38:31,070 --> 00:38:34,850
+المسافة ما بين الـ two objects، ممكن حسابها بهذا
+
+498
+00:38:34,850 --> 00:38:41,640
+القانون، تمام؟ والـ … هنا عملية ربط. The
+
+499
+00:38:41,640 --> 00:38:45,300
+larger numerical aperture، إيش يعني larger numerical
+
+500
+00:38:45,300 --> 00:38:48,200
+aperture؟ يعني كمية الضوء داخلة للعدسة الشيئية
+
+501
+00:38:48,200 --> 00:38:53,320
+كبيرة. وحكينا أن العدسة الشيئية اللي قوة تكبيرها
+
+502
+00:38:53,320 --> 00:38:57,060
+كبيرة، اللي magnification تبعها كبير، هي اللي بدها
+
+503
+00:38:57,060 --> 00:39:02,670
+larger NA، تمام؟ بدها larger numerical aperture. طيب،
+
+504
+00:39:02,670 --> 00:39:08,710
+فمعناها هيكون هذا الـ numerical
+
+505
+00:39:08,710 --> 00:39:11,230
+aperture، وهذه العدسة اللي قوة تكبيرها
+
+506
+00:39:11,230 --> 00:39:16,530
+كبيرة، بيوضح لي smaller distance between objects.
+
+507
+00:39:16,530 --> 00:39:21,410
+هيوضح لي مسافات… يعني يفصل لي الـ objects أو النقاط
+
+508
+00:39:21,410 --> 00:39:27,030
+اللي ما بينهم مسافات قليلة جداً، تمام؟ إذا هو عنده
+
+509
+00:39:27,030 --> 00:39:32,030
+efficient أو greater resolving power، تمام؟ بيزيد
+
+510
+00:39:32,030 --> 00:39:35,430
+الـ numerical aperture، بيزيد كمية الضوء اللي داخلة
+
+511
+00:39:35,430 --> 00:39:39,410
+للعدسة الشيئية. إذا العدسة الشيئية نفسها، هتكون قوة
+
+512
+00:39:39,410 --> 00:39:43,130
+تكبيرها كبيرة، عشان هي اللي بتحتاج ضوء أكبر.
+
+513
+00:39:43,130 --> 00:39:48,510
+إذا قوة التوضيح هتكون أكبر، وهيوضح لي الـ objects اللي
+
+514
+00:39:48,510 --> 00:39:51,450
+بينهم مسافات أصغر، تمام؟
+
+515
+00:39:54,070 --> 00:39:57,530
+working principle of an oil immersion، مبدأ عمل الـ
+
+516
+00:39:57,530 --> 00:40:02,550
+oil immersion، كيف إحنا العدسة الـ 100x أو الـ
+
+517
+00:40:02,550 --> 00:40:08,010
+oil immersion lens، كيف بتشتغل؟ شوفوا معايا، نرجع لـ
+
+518
+00:40:08,010 --> 00:40:14,570
+الميكروسكوب ونتخيل مع بعض مسارات الضوء، مسارات الضوء.
+
+519
+00:40:16,490 --> 00:40:21,930
+طيب، الضوء ينتقل من الـ light source، ممكن يمر بمرآة
+
+520
+00:40:21,930 --> 00:40:27,590
+أو بـ filter، وبعدين بالقرص بيتحكم بكمية وجوده أو
+
+521
+00:40:27,590 --> 00:40:31,670
+كميته اللي داخلة للـ condenser، والـ condenser
+
+522
+00:40:31,670 --> 00:40:37,010
+بيجمعه وبيخليه يعني يفوت في العدسة الشيئية اللي
+
+523
+00:40:37,010 --> 00:40:42,220
+هي 100X أو الـ oil immersion. وإحنا متفقين أنه بين
+
+524
+00:40:42,220 --> 00:40:47,280
+أن الـ oil immersion هي أكبر عدسة أو أكبر قوة تكبير
+
+525
+00:40:47,280 --> 00:40:50,320
+في الـ microscope، في الـ light microscope، هذا
+
+526
+00:40:50,320 --> 00:40:54,660
+تمام؟ الميكروسكوب الضوئي اللي إحنا بنشرحه، تمام؟
+
+527
+00:40:54,660 --> 00:41:01,050
+فمعناها أنها محتاجة أكبر كمية ضوء. طيب، الضوء يمر
+
+528
+00:41:01,050 --> 00:41:06,750
+بأوساط مختلفة: glass اللامبة، هواء، الـ glass الـ…
+
+529
+00:41:06,750 --> 00:41:07,970
+الـ…الـ…الـ…الـ…الـ…الـ…الـ…الـ…
+
+530
+00:41:07,970 --> 00:41:08,250
+الـ…الـ…الـ…الـ…الـ…الـ…الـ…الـ…
+
+531
+00:41:08,250 --> 00:41:11,670
+الـ…الـ…الـ…الـ…الـ…الـ…الـ…الـ…
+
+532
+00:41:11,670 --> 00:41:12,430
+الـ…الـ…الـ…الـ…الـ…الـ…الـ…الـ…
+
+533
+00:41:12,430 --> 00:41:12,450
+الـ…الـ…الـ…الـ…الـ…الـ…الـ…الـ…
+
+534
+00:41:12,450 --> 00:41:12,970
+الـ…الـ…الـ…الـ…الـ…الـ…الـ…الـ…
+
+535
+00:41:12,970 --> 00:41:13,030
+الـ…الـ…الـ…الـ…الـ…الـ…الـ…الـ…
+
+536
+00:41:13,030 --> 00:41:13,750
+الـ…الـ…الـ…الـ…الـ…الـ…الـ…الـ…
+
+537
+00:41:13,750 --> 00:41:14,830
+الـ…الـ…الـ…الـ…الـ…الـ…الـ…الـ…
+
+538
+00:41:14,830 --> 00:41:14,850
+الـ…الـ…الـ…الـ…الـ…الـ…الـ…الـ…
+
+539
+00:41:14,850 --> 00:41:19,150
+الـ…
+
+540
+00:41:19,150 --> 00:41:23,430
+الـ…الـ…الـ…
+
+541
+00:41:29,750 --> 00:41:35,750
+للعدسة، مية مش كثيرة كما ينبغي، تمام؟ مش كثيرة كما
+
+542
+00:41:35,750 --> 00:41:39,810
+ينبغي. ليش؟ لأن جزء من هذا الضوء يتشتت نتيجة
+
+543
+00:41:39,810 --> 00:41:45,850
+انتقاله في أوساط مختلفة وانكساراته، تمام؟ طيب، طب
+
+544
+00:41:45,850 --> 00:41:49,370
+إيش إحنا نعمله علشان إحنا نحافظ على كمية الضوء هذه
+
+545
+00:41:49,370 --> 00:41:52,990
+أنها كلها تفوت للعدسة الشيئية أو ما يتم تشتت هذا
+
+546
+00:41:52,990 --> 00:41:58,430
+الضوء؟ شوفوا معايا، إيش بنساوي؟ نرجع بس للـ slide.
+
+547
+00:42:01,070 --> 00:42:07,110
+هي هنا، الضوء بيمر من زجاج إلى هواء إلى زجاج، مرة…
+
+548
+00:42:07,110 --> 00:42:11,350
+إلى هواء، مرة أخرى إلى زجاج، فإيش؟ بيحكي لنا أنه
+
+549
+00:42:11,350 --> 00:42:16,390
+بيتشتت، تمام؟ طيب، لكن لو أنا حطيت على الشريحة تبعي
+
+550
+00:42:16,390 --> 00:42:23,080
+هذه هي الشريحة، لو حطيت عليها قطرة زيت، oil، غمرتها بـ
+
+551
+00:42:23,080 --> 00:42:27,180
+oil، فصارت oil، وطبعاً المسافة اللي ما بين…
+
+552
+00:42:27,180 --> 00:42:30,220
+المسافة اللي ما بين العدسة الـ 100x، يا بنات، والـ
+
+553
+00:42:30,220 --> 00:42:34,860
+slide، بتكون كثيرة قليلة، طبعاً. فإذا، المساحة أو
+
+554
+00:42:34,860 --> 00:42:38,640
+المسافة ما بينهم بقطرة oil، فساعتها العدسة هذه
+
+555
+00:42:38,640 --> 00:42:42,140
+بتكون عدسة مغمورة بالزيت، عشان هيك إحنا بنحكي عنها
+
+556
+00:42:42,140 --> 00:42:46,420
+إما 100x أو بنحكي عنها الـ oil immersion، العدسة اللي
+
+557
+00:42:46,420 --> 00:42:52,360
+تغمر بالزيت، طبعاً. طيب، إيش اللي هيصير بهذه الوضع؟ لو
+
+558
+00:42:52,360 --> 00:42:59,480
+أنا حطيت oil immersion، فمعناها أن الزيت هذا اللي أنا حطيته
+
+559
+00:42:59,480 --> 00:43:03,860
+له نفس معامل انكسار
+
+560
+00:43:03,860 --> 00:43:09,470
+الزجاج، فبيصير الضوء بيمر من الـ light source، بيروح
+
+561
+00:43:09,470 --> 00:43:13,770
+على الزجاج اللي هو زجاج الـ condenser، بعدين زجاج
+
+562
+00:43:13,770 --> 00:43:17,890
+الشريحة، بعدين بيمر في المنطقة هذه اللي هي مغمورة
+
+563
+00:43:17,890 --> 00:43:21,410
+بالـ oil اللي له نفس معامل انكسار الزجاج، يعني إزاي
+
+564
+00:43:21,410 --> 00:43:26,250
+كأنه الضوء ماشي بزجاج أصلاً، تمام؟ وينتقل إلى العدسة
+
+565
+00:43:26,250 --> 00:43:30,610
+الشيئية اللي هي أيضاً مصنوعة من الزجاج. إذا أصبح
+
+566
+00:43:30,610 --> 00:43:34,630
+إحنا هيك وحدنا مسارات الضوء، أو كأنه وحدنا مسارات
+
+567
+00:43:34,630 --> 00:43:38,330
+باستخدام الزيت اللي له نفس معامل انكسار الزجاج،
+
+568
+00:43:38,330 --> 00:43:42,230
+فزي كأنه الضوء بيمر بنفس المسار من زجاج
+
+569
+00:43:42,230 --> 00:43:46,210
+الـ condenser إلى الـ slide إلى الـ oil إلى زجاج
+
+570
+00:43:46,210 --> 00:43:52,510
+العدسة الشيئية. يعني، وهيك بنكون إحنا وقفنا تشتت
+
+571
+00:43:52,510 --> 00:43:56,890
+للضوء. جمعنا الضوء، جمعناه كله لحتى يفوت في العدسة
+
+572
+00:43:56,890 --> 00:44:01,310
+الشيئية. ليش؟ لأنه إحنا مستخدمين قوة تكبير كبيرة، عدسة
+
+573
+00:44:01,310 --> 00:44:06,570
+100X، فمحتاجين كمية الضوء أكبر، محتاجين أن الـ NA، الـ
+
+574
+00:44:06,570 --> 00:44:10,950
+numerical aperture، يكون أعلى، تمام؟ ومش محتاجين ولا
+
+575
+00:44:10,950 --> 00:44:15,600
+شيء في الضوء يتشتت، تمام؟ ما بدي أي ضوء يتشتت. فهي هي
+
+576
+00:44:15,600 --> 00:44:18,900
+فكرة الـ oil immersion، الضوء يتشتت نتيجة مروره
+
+577
+00:44:18,900 --> 00:44:23,640
+بمسارات مختلفة. مع وجود الـ oil، الضوء رح ينحجب، و
+
+578
+00:44:23,640 --> 00:44:30,220
+رح يظل كأنه… زي كأنه ماشي بمسار واحد، فبالتالي كله
+
+579
+00:44:30,220 --> 00:44:33,600
+هيفوت في الـ objective، وهيك هيكون في عندي
+
+580
+00:44:33,600 --> 00:44:38,480
+قوة إيضاح أفضل وتكبير أفضل. نشوف الكلام اللي مكتوب
+
+581
+00:44:38,480 --> 00:44:42,300
+هنا، هو نفسه اللي شرحنا. و in a beam of light، لما
+
+582
+00:44:42,300 --> 00:44:47,080
+تكون عندي حزمة ضوء، passes from air into glass، it
+
+583
+00:44:47,080 --> 00:44:50,860
+is bent، تمام؟ لما بتنتقل من الهواء اللي موجود تحت
+
+584
+00:44:50,860 --> 00:44:54,220
+الـ condenser للـ glass اللي في الـ condenser، بتنكسر.
+
+585
+00:44:54,580 --> 00:44:58,320
+طبعاً، and when it passes back from glass to air، و
+
+586
+00:44:58,320 --> 00:45:01,560
+لما أيضاً كمان هذا الضوء ينتقل من الـ glass تبع
+
+587
+00:45:01,560 --> 00:45:08,060
+الـ condenser والهواء، المسافة اللي ما بين الشريحة و
+
+588
+00:45:08,060 --> 00:45:14,300
+العدسة، أيضاً كمان بينكسر مرة ثانية، فبالتالي لما
+
+589
+00:45:14,300 --> 00:45:19,310
+بيرجع لمساره بيكون تشتت جزء منه. لما يمر على مسار
+
+590
+00:45:19,310 --> 00:45:23,570
+أو بيمشي في مساره باتجاه العدسة الشيئية، بيكون إحنا
+
+591
+00:45:23,570 --> 00:45:27,510
+فقدنا جزء من هذا الضوء نتيجة انكساراته. ينكسر ويرجع،
+
+592
+00:45:27,510 --> 00:45:32,230
+ينعكس، ينكسر ويرجع ينعكس. إذا… إذا اتشتت، تمام؟
+
+593
+00:45:32,230 --> 00:45:37,490
+طيب، فهذا هيأثر على الـ oil immersion objective، ليش؟
+
+594
+00:45:37,490 --> 00:45:40,510
+لأنه هيأثر على الـ numerical aperture، كمية الضوء
+
+595
+00:45:40,510 --> 00:45:44,110
+اللي داخلة على الـ objective. وإحنا متفقين، وكررنا
+
+596
+00:45:44,110 --> 00:45:48,270
+مراراً وتكراراً، أنه والله كل ما استخدمنا قوة تكبير
+
+597
+00:45:48,270 --> 00:45:52,510
+كبيرة، معناها بدي ضوء كبير، بدي كمية ضوء أعلى تصل
+
+598
+00:45:52,510 --> 00:45:57,390
+أو أكبر، تصل إلى الـ objective lens، تمام؟ فإذا أيضاً
+
+599
+00:45:57,390 --> 00:46:00,770
+هتأثر على الـ resolving power، على قوة الإيضاح لهذه
+
+600
+00:46:00,770 --> 00:46:06,030
+العدسة. فبالتالي، المفروض أن أنا أمنع انكسار الضوء
+
+601
+00:46:06,030 --> 00:46:14,420
+هذا من خلال أن أنا أحل الهواء اللي موجود… الهواء
+
+602
+00:46:14,420 --> 00:46:21,280
+اللي موجود بين العينة والعدسة، أشغله بقطرة
+
+603
+00:46:21,280 --> 00:46:27,140
+زيت، تمام؟ هذا الزيت… هذا الزيت له the same
+
+604
+00:46:27,140 --> 00:46:30,600
+optical properties of glass، له نفس الخصائص
+
+605
+00:46:30,600 --> 00:46:36,280
+الضوئية أو الخصائص للزجاج، يعني له نفس معامل
+
+606
+00:46:36,280 --> 00:46:42,980
+انكسار الضوء في الزجاج. فإذا، لما الضوء بيطلع من
+
+607
+00:46:42,980 --> 00:46:48,000
+الـ condenser، بيمر بالـ slide، وبعدين بيمر بالزيت،
+
+608
+00:46:48,000 --> 00:46:50,960
+نفس معامل انكسار الزجاج، ده، يعني ذلك أنه ينتقل من
+
+609
+00:46:50,960 --> 00:46:54,820
+زجاج إلى زجاج إلى ما يشبه الزجاج، اللي هو الـ oil، ومن
+
+610
+00:46:54,820 --> 00:47:01,530
+ثم إلى الـ oil immersion، العدسة اللي هي أيضاً زجاج.
+
+611
+00:47:01,530 --> 00:47:06,670
+إذا أنا هيك وحدت مسارات الضوء، منعت تشتته
+
+667
+00:51:27,680 --> 00:51:31,720
+مصبوغة بـ fluorescent dye وبشوفها لامعة تحت الـ
+
+668
+00:51:31,720 --> 00:51:35,160
+microscope تمام؟ هذه فكرة الـ fluorescent
+
+669
+00:51:35,160 --> 00:51:39,790
+microscope. طيب، الـ dark field microscope، الـ dark
+
+670
+00:51:39,790 --> 00:51:43,470
+field microscope هو عبارة عن microscope يعطيني
+
+671
+00:51:43,470 --> 00:51:49,330
+black background، اللي هو خلفية معتمة للصورة أو
+
+672
+00:51:49,330 --> 00:51:53,930
+للعينة، وفقط البكتيريا أو الخلية أو العنصر المراد
+
+673
+00:51:53,930 --> 00:51:58,760
+رؤيته هو اللي بيكون مضئًا. تمام؟ الـ suspended bacteria
+
+674
+00:51:58,760 --> 00:52:01,740
+أو الـ element، العنصر اللي مراد رؤيته هو اللي بيكون
+
+675
+00:52:01,740 --> 00:52:08,100
+bright، لامعًا ومضئًا. تمام؟ كيف يتم تصوير بهذا الـ...
+
+676
+00:52:08,100 --> 00:52:12,480
+بهذه الطريقة؟ الـ... الـ microscope هذا عنده special
+
+677
+00:52:12,480 --> 00:52:17,260
+condenser. هذا الـ condenser بيجمع الضوء تمامًا، وفقط
+
+678
+00:52:17,260 --> 00:52:20,840
+بيخليه يخترق مين؟ بيخليه يخترق الـ object اللي على
+
+679
+00:52:20,840 --> 00:52:25,360
+الـ... على العدسة، على الـ slide. تمام؟ بيخترق الـ... الـ
+
+680
+00:52:25,360 --> 00:52:29,250
+... الـ... الـ... فقط اللي هو البكتيريا أو الـ
+
+681
+00:52:29,250 --> 00:52:33,810
+element المراد رؤيته على الـ slide، وينتقل الضوء منه
+
+682
+00:52:33,810 --> 00:52:37,570
+إلى العدسة الشيئية. أي شيء آخر، في حوالين هذا الـ
+
+683
+00:52:37,570 --> 00:52:42,110
+... الـ element، يتبدد. فإحنا بنشوف كل شيء معتم إلا
+
+684
+00:52:42,110 --> 00:52:47,360
+الخلية أو العنصر المراد رؤيته بيكون مضئًا، تمام؟ فـ
+
+685
+00:52:47,360 --> 00:52:50,560
+... وعندي نوع رابع اللي هو الـ face contrast
+
+686
+00:52:50,560 --> 00:52:54,600
+مايكروسكوب. الـ face contrast مايكروسكوب هو طبعًا
+
+687
+00:52:54,600 --> 00:52:58,460
+في الأنواع السابقة الذكر، إحنا بنشوف الخلايا و
+
+688
+00:52:58,460 --> 00:53:03,700
+البكتيريا والفطريات والطلائعيات، أي خلايا سواء
+
+689
+00:53:03,700 --> 00:53:08,160
+كانت طبعًا يعني animal cells or plant cells أو حتى
+
+690
+00:53:08,160 --> 00:53:16,320
+كمان الـ prokaryotes. تمام؟ بنشوفها وهي ميتة، الخلايا
+
+691
+00:53:16,320 --> 00:53:20,960
+يعني لابد من قتلها وتجفيفها وصباغتها. should be
+
+692
+00:53:20,960 --> 00:53:25,760
+killed, dried, and stained. وصباغتها عشان نتمكن من
+
+693
+00:53:25,760 --> 00:53:28,540
+رؤيتها. لكن هذا الـ microscope اللي هو الـ face
+
+694
+00:53:28,540 --> 00:53:33,520
+contrast microscope بيُرْجيني
+
+695
+00:53:33,520 --> 00:53:37,970
+الخلايا. visualize the living bacteria and cells.
+
+696
+00:53:37,970 --> 00:53:42,070
+تمام؟ بيُرْجيني الخلايا وهي حية. كيف؟ والله إن أنا
+
+697
+00:53:42,070 --> 00:53:46,450
+في عندي معامل، معامل انكسار مختلفة لمرور الضوء أثناء
+
+698
+00:53:46,450 --> 00:53:55,390
+مروره خلال كائن حي، وأثناء مرور الضوء في كائن أو خلية
+
+699
+00:53:55,390 --> 00:54:00,130
+ميتة، تمام؟ فهذا الـ... الـ... الـ microscope يوظف
+
+700
+00:54:00,130 --> 00:54:04,650
+هذا الفرق في معامل الانكسار للضوء، تمام؟ إذا
+
+701
+00:54:04,650 --> 00:54:07,890
+معامل الانكسار للضوء يختلف أثناء مروره في خلية
+
+702
+00:54:07,890 --> 00:54:12,580
+حية عن مروره في خلية ميتة. هذا الـ microscope enhances
+
+703
+00:54:12,580 --> 00:54:16,820
+بيعزز أو يستخدم الـ slight difference، هذا الفرق ما
+
+704
+00:54:16,820 --> 00:54:19,760
+بين الـ... الـ... الـ... هذا الفرق في الـ refractive
+
+705
+00:54:19,760 --> 00:54:24,120
+index، في معامل الانكسار، ما بين مروره في خلايا حية
+
+706
+00:54:24,120 --> 00:54:27,840
+وما بين مروره في الوسط أو الخلايا الميتة المحيطة
+
+707
+00:54:27,840 --> 00:54:34,560
+بها. فبالتالي بيوضح لي الـ... الخلايا الحية. الإلكترون
+
+708
+00:54:34,560 --> 00:54:38,580
+مايكروسكوب، طبعًا هو تقريبًا أفضل مايكروسكوب وأعلى
+
+709
+00:54:38,580 --> 00:54:43,540
+قوة تكبير. لمايكروسكوب بتقريبًا بيعطيني قوة تكبير
+
+710
+00:54:43,540 --> 00:54:50,120
+1500x أو 2000x، وفي قوة عند خمسين ألف x. تمام؟
+
+711
+00:54:50,120 --> 00:54:51,300
+بيكون كبير.
+
+712
+00:54:54,250 --> 00:54:59,850
+الإلكترون مايكروسكوب، فيه منه نوعين، طبعًا فيه الـ
+
+713
+00:54:59,850 --> 00:55:02,850
+transmission electron microscope، بيعطيني صورة
+
+714
+00:55:02,850 --> 00:55:06,490
+ثنائية الأبعاد، وفيه scanning electron microscope
+
+715
+00:55:06,490 --> 00:55:14,650
+اللي هو ثلاثي الأبعاد، تمام؟ فهو عنده يعني... بـ...
+
+716
+00:55:14,650 --> 00:55:16,990
+بـ... إحنا... إحنا بنفضل نستخدمه عن الـ light
+
+717
+00:55:16,990 --> 00:55:20,210
+microscope. ليش؟ لأن الـ light microscope يعني مُقْصِّر
+
+718
+00:55:20,210 --> 00:55:24,550
+فيه، عنده decreased efficiency و resolving power،
+
+719
+00:55:24,550 --> 00:55:28,390
+تمام؟ لكن هو... هذا عنده high degree of resolving
+
+720
+00:55:28,390 --> 00:55:33,900
+power. الـ electron microscopy. طيب، routine use of
+
+721
+00:55:33,900 --> 00:55:37,400
+microscope. هذه الـ... النقاط أنا راح أذكرها بشكل
+
+722
+00:55:37,400 --> 00:55:42,300
+سريع، ولكن راح أشرحها بالتفصيل داخل المختبر، على
+
+723
+00:55:42,300 --> 00:55:46,500
+المختبر... على الـ... الـ microscope نفسه، تمام؟ فـ
+
+724
+00:55:46,500 --> 00:55:50,260
+... لحتى يكون أسهل وأوضح. أول شيء، أنا لما بدي أجيء
+
+725
+00:55:50,260 --> 00:55:53,280
+أستخدم الـ microscope، لازم أتأكد إن هو موجود على
+
+726
+00:55:53,280 --> 00:55:56,560
+firm bench، على bench ثابت. ما فيش... الـ bench هذا مش
+
+727
+00:55:56,560 --> 00:56:00,660
+موجود جنب جهاز تبريد أو centrifuge أو شيء بيعملي
+
+728
+00:56:00,660 --> 00:56:05,300
+اهتزازات، لأن هذا هيأثر على وضوح الصورة، تمام؟ و make
+
+729
+00:56:05,300 --> 00:56:08,140
+sure that it is not exposed to direct sunlight. إنه
+
+730
+00:56:08,140 --> 00:56:13,200
+هذا الجهاز مش لازم يتعرض لأشعة الشمس مباشرة لأنه
+
+731
+00:56:13,200 --> 00:56:20,380
+ممكن يَتَلَف، يَتَصَدَّع، يخرب. وأيضًا الـ user، المستخدم
+
+732
+00:56:20,380 --> 00:56:24,680
+للـ microscope لازم يجلس على ارتفاع مناسب من الـ
+
+733
+00:56:24,680 --> 00:56:28,260
+microscope. should be seated at the correct height.
+
+734
+00:56:28,260 --> 00:56:35,650
+يعني على ارتفاع مناسب من الـ microscope. select the
+
+735
+00:56:35,650 --> 00:56:40,310
+appropriate source of light. لازم نختار مصدر الضوء
+
+736
+00:56:40,310 --> 00:56:45,950
+سواء كان لمبة أو daylight، وبشغل الجهاز بالكهرباء،
+
+737
+00:56:45,950 --> 00:56:48,870
+بضبط الضوء من عند الـ knob اللي حكيت لكم عنها، بتحكم
+
+738
+00:56:48,870 --> 00:56:54,260
+بشدة الإضاءة، ومن أيضًا المكان اللي أشرت لكم إليه في
+
+739
+00:56:54,260 --> 00:57:00,260
+أول الـ slides. وطبعًا بحط العينة بتاعتي على الـ stage،
+
+740
+00:57:00,260 --> 00:57:05,620
+بثبتها بالـ clips. وطبعًا بتأكد إن الـ slide من تحت
+
+741
+00:57:05,620 --> 00:57:10,500
+طبعًا تكون ناشفة، لأن أي بقايا صبغة أو وسائل راح
+
+742
+00:57:10,500 --> 00:57:15,460
+يأثر على وضوح الصورة. فبمسحها قبل ما أحطها على الـ
+
+743
+00:57:15,460 --> 00:57:20,040
+slide، على الـ stage. select the objective to be used.
+
+744
+00:57:22,490 --> 00:57:28,510
+بختار العدسة الشيئية المراد استخدامها. وهنا يفضل يا
+
+745
+00:57:28,510 --> 00:57:32,410
+بنات، إذا أنا بدي أستخدم قوة تكبير كبيرة، يفضل إن
+
+746
+00:57:32,410 --> 00:57:36,010
+أنا أبدأ بقوة تكبير صغيرة، بعدين أنتقل لكبيرة. it's
+
+747
+00:57:36,010 --> 00:57:38,490
+better to begin examination with low power with
+
+748
+00:57:38,490 --> 00:57:43,850
+a 10x، مثلًا، تمام؟ وطبعًا ليش... ليش أنا بـ... ببدأ في
+
+749
+00:57:43,850 --> 00:57:46,510
+الـ 10x؟ عشان أـ... أظبط الـ... الـ... الـ
+
+750
+00:57:46,510 --> 00:57:51,930
+illumination، الإضاءة، أجيب الـ field، أجيب الصورة،
+
+751
+00:57:51,930 --> 00:57:57,450
+تمام؟ أتأكد إن كل الأمور تمام، بعدين أنتقل
+
+752
+00:57:57,450 --> 00:58:00,410
+للـ high power field، اللي هي 40x أو حتى oil
+
+753
+00:58:00,410 --> 00:58:05,520
+immersion، ممكن أنتقل إليها. طبعًا، كيف أنا بجيب الـ
+
+754
+00:58:05,520 --> 00:58:09,940
+field؟ bring the objective as close as possible to
+
+755
+00:58:09,940 --> 00:58:15,780
+the slide preparation. يعني أنا بقرب المسافة ما بين
+
+756
+00:58:15,780 --> 00:58:20,160
+الـ stage اللي عليها الـ slide وما بين الـ objective
+
+757
+00:58:20,160 --> 00:58:25,100
+lenses. كيف؟ بحرك الـ coarse adjustment. مجرد ما أجيب
+
+758
+00:58:25,100 --> 00:58:29,720
+coarse adjustment، ممكن أنا أتحكم بكمية الضوء اللي
+
+759
+00:58:29,720 --> 00:58:34,540
+مارة من خلال إن أنا أعمل adjustment للـ light وـ...
+
+760
+00:58:34,540 --> 00:58:40,630
+وـ... للـ condenser. ممكن أفتح القرص أو أسكره حسب كمية
+
+761
+00:58:40,630 --> 00:58:43,130
+الضوء اللي بضبطها، وحسب نوع العدسة اللي أنا
+
+762
+00:58:43,130 --> 00:58:47,330
+بستخدمها. وطبعًا، حكينا على الـ low power، القرص يفضل
+
+763
+00:58:47,330 --> 00:58:50,990
+أنه يكون مغلق، وبفتحه على الـ high power والـ oil
+
+764
+00:58:50,990 --> 00:58:55,490
+immersion. طيب، وطبعًا هنا هي بيحكي لي the wider the
+
+765
+00:58:55,490 --> 00:59:00,670
+condenser aperture. كلما زادت وسعت فتحة الـ aperture،
+
+766
+00:59:00,670 --> 00:59:04,990
+فتحة الحجاب، اللي... اللي تحت الـ condenser، طبعًا
+
+767
+00:59:04,990 --> 00:59:09,110
+معناها كمية ضوء أكبر هتفوت للـ condenser، معناها
+
+768
+00:59:09,110 --> 00:59:13,650
+الصورة إيش هتكون؟ brighter، مضيئة أكثر ولامعة أكثر.
+
+769
+00:59:13,650 --> 00:59:18,610
+طبعًا، وأحيانًا... أحيانًا بتكون الـ details قليلة
+
+770
+00:59:18,610 --> 00:59:23,250
+اللي بنشوفها في هذه الحالة، إلا إذا كنت بستخدم، يعني
+
+771
+00:59:23,250 --> 00:59:27,890
+عدسة كبيرة. فلأ يعني الـ wider condenser هيعطيني
+
+772
+00:59:27,890 --> 00:59:33,830
+brighter، وبرده كمان أوضح. فعشان هيك لازم إن أنا
+
+773
+00:59:33,830 --> 00:59:37,830
+أسكره في العدسات اللي قوة تكبيرها قليلة، لأن ممكن
+
+774
+00:59:37,830 --> 00:59:40,590
+تكون الـ details مش واضحة كتير. يعني أنا بس شايفها
+
+775
+00:59:40,590 --> 00:59:44,650
+صورة لامعة فقط لا غير، تمام؟ إذا في هذه النقطتين
+
+776
+00:59:44,650 --> 00:59:48,350
+ينطبقان على العدسة 10x، إنه كل ما كان
+
+777
+00:59:48,350 --> 00:59:50,830
+الـ condenser فاتح أكثر، يعني ضوء أكثر، يعني
+
+778
+00:59:50,830 --> 00:59:53,870
+الـ details ما بشوفهاش كتير، بتكون الصورة بس لامعة
+
+779
+00:59:53,870 --> 00:59:57,750
+على الفاضي، تمام؟ فإذا كان الـ smaller aperture،
+
+780
+00:59:57,750 --> 01:00:02,150
+يعني greater will be contrast، يعني في تباين في
+
+781
+01:00:02,150 --> 01:00:06,530
+الصورة، فبتكون واضحة أكثر. هذا ينطبق على 10x، لكن
+
+782
+01:00:06,530 --> 01:00:11,150
+على العدسات اللي قوة تكبيرها أعلى، العكس، يعني أنا
+
+783
+01:00:11,150 --> 01:00:13,850
+محتاجة wider condenser ومحتاجة ضوء أكثر.
+
+784
+01:00:16,710 --> 01:00:20,330
+تمام؟ عادة الصورة، كيف أنا بشوفها؟ بشوفها upside
+
+785
+01:00:20,330 --> 01:00:26,450
+down، مقلوبة ومعكوسة كمان. upside down and in
+
+786
+01:00:26,450 --> 01:00:29,110
+opposite direction. يعني اللي تحت فوق، واللي فوق
+
+787
+01:00:29,110 --> 01:00:33,710
+تحت، وعلى المعكوسة.
+
+788
+01:00:33,710 --> 01:00:40,510
+يعني اليمين يسار، واليسار يمين. عشان هيك في مكان
+
+789
+01:00:40,510 --> 01:00:43,510
+لتحريك الشريحة
+
+790
+01:00:44,850 --> 01:00:52,430
+على الـ stage، هنا هنا.
+
+791
+01:00:52,430 --> 01:00:58,290
+هادول... هادول الاثنين بيحركولي الشريحة، طبعًا؟
+
+792
+01:00:58,290 --> 01:01:04,130
+فواحد بيحركها لفوق وتحت، وواحد بيحركها يمين يسار،
+
+793
+01:01:04,130 --> 01:01:09,410
+طبعًا؟ فلما أنا بكون بروح يمين على... لما بحرك
+
+794
+01:01:09,410 --> 01:01:13,210
+الشريحة
+
+795
+01:01:13,210 --> 01:01:17,350
+إلى اليمين على الـ stage، معناه أنا بكون بروح لليسار
+
+796
+01:01:17,350 --> 01:01:23,290
+في الصورة. ولما بحرك الـ... الـ... هدول، يعني بحرك الـ
+
+797
+01:01:23,290 --> 01:01:26,890
+... الـ slide إلى اليسار على الـ stage، معناها أنا
+
+798
+01:01:26,890 --> 01:01:30,010
+بروح لليمين في الصورة، لأن الصورة معكوسة. ونفس
+
+799
+01:01:30,010 --> 01:01:33,490
+الشيء، لو أنا كنت بطلع لفوق على الـ... في الـ... في
+
+800
+01:01:33,490 --> 01:01:37,450
+الـ slide، تمام؟ معناها أنا بكون بنزل لتحت في الصورة.
+
+801
+01:01:37,450 --> 01:01:41,420
+ولما بنزل لتحت في الـ slide، بكون بطلع لفوق في
+
+802
+01:01:41,420 --> 01:01:44,700
+الصورة، لأن الصورة بتكون upside-down. إذا، الاتجاهات
+
+803
+01:01:44,700 --> 01:01:47,380
+بتكون معكوسة على الـ microscope. وهذه شغل... شغل
+
+804
+01:01:47,380 --> 01:01:51,820
+لازم ننتبه لها كثير منيح، وهنتعلمها مع الممارسة.
+
+805
+01:02:00,350 --> 01:02:05,210
+تمام؟ بعد هيك ممكن أصل لـ... يعني أغير على 40x بعد
+
+806
+01:02:05,210 --> 01:02:10,090
+ما جبت الصورة باستخدام الـ 10x، وأبدأ أفحص الـ... الـ
+
+807
+01:02:10,090 --> 01:02:15,370
+... العينة بتاعتي. وممكن أنا إذا محتاجة oil
+
+808
+01:02:15,370 --> 01:02:22,170
+immersion أو 100x lenses، فبحط قطرة زيت على الشريحة
+
+809
+01:02:22,170 --> 01:02:28,870
+بتاعتي، علشان أتمكن من شو... رؤية الأجزاء والصورة
+
+810
+01:02:28,870 --> 01:02:32,670
+موضحة أكثر. هي عندي طبعًا الـ oil immersion، هنستخدمها
+
+811
+01:02:32,670 --> 01:02:36,190
+لما تعملوا الـ blood smear، مسحة الدم، وهتستخدموها في
+
+812
+01:02:36,190 --> 01:02:40,630
+مختبرات الـ microbiology، لما تستخدموا الـ asbestos
+
+813
+01:02:40,630 --> 01:02:46,010
+ثاني، اللي هي صبغة لصباغة البكتيريا. وكل ما بتكونوا
+
+814
+01:02:46,010 --> 01:02:49,730
+تَصبغوا بكتيريا أو خلايا، راح تستخدموا الـ oil
+
+815
+01:02:49,730 --> 01:02:54,880
+immersion. طيب، care, cleaning and repair of the
+
+816
+01:02:54,880 --> 01:02:57,640
+microscope. كيف أنا أحافظ على الـ microscope؟ أول
+
+817
+01:02:57,640 --> 01:03:03,140
+شيء، الـ microscope طبعًا معرض للتلف، فلازم أنا
+
+818
+01:03:03,140 --> 01:03:07,020
+أراعي أو يكون في عندي اعتبارات تُراعَى أثناء
+
+819
+01:03:07,020 --> 01:03:10,910
+استخدامه. أول شيء، بدي أمسكه بإيدين اثنتين، ممنوع أمسكه
+
+820
+01:03:10,910 --> 01:03:14,690
+بإيد واحدة، لإنه ممكن يعني إذا وقع خلاص، يخرب، يتكسر.
+
+821
+01:03:14,690 --> 01:03:18,310
+و not in use، إذا أنا ما استخدمته، فمعناها إن أنا
+
+822
+01:03:18,310 --> 01:03:22,750
+لازم أحميه، أول شيء من الغبار، من الرطوبة، من الـ
+
+823
+01:03:22,750 --> 01:03:29,370
+sunlight. فيعني إله بيكون sack، إله حقيبة بلاستيكية
+
+824
+01:03:29,370 --> 01:03:33,390
+أو بـ case أو cover بيغطيه. فلازم أكون مغطية، أكون
+
+825
+01:03:33,390 --> 01:03:37,630
+مفصلة من الكهرباء. مش لازم يضلّ مُشبَك بالكهرباء، ولازم
+
+826
+01:03:37,630 --> 01:03:44,420
+يكون طبعًا... إذا كان أنا... إذا بدي أخليه في مكان
+
+827
+01:03:44,420 --> 01:03:48,680
+الشغل جاهز، يعني لأي وقت بستخدمه فيه، بس على الأقل
+
+828
+01:03:48,680 --> 01:03:53,020
+يعني أغطيه بالـ cover بتاعه، تمام؟ في الـ humidity
+
+829
+01:03:53,020 --> 01:03:56,960
+climate، في المناطق اللي الجو بتاعها رطب، ممكن إنه
+
+830
+01:03:56,960 --> 01:03:59,880
+يَتَصَدَّع هذا الـ microscope، نتيجة تكوين قطرات ندى عليه،
+
+831
+01:03:59,880 --> 01:04:04,420
+وهذا فبيَتَصَدَّع. فيفضل إن الـ cover بتاعه اللي هو الـ
+
+832
+01:04:04,420 --> 01:04:08,160
+plastic bag بتاعته اللي هو الكيس أو الحقيبة اللي
+
+833
+01:04:08,160 --> 01:04:14,850
+بتغطيه، إنه تكون عليها silica gel، مادة silica gel،
+
+834
+01:04:14,850 --> 01:04:18,970
+وهي مادة drying agent، عامل مجفف، يمتص الرطوبة، عشان
+
+835
+01:04:18,970 --> 01:04:25,03
\ No newline at end of file
diff --git a/PL9fwy3NUQKwY9jl8xw-bWkVZkWcGGyspd/Eut_8KsMs84_postprocess.srt b/PL9fwy3NUQKwY9jl8xw-bWkVZkWcGGyspd/Eut_8KsMs84_postprocess.srt
new file mode 100644
index 0000000000000000000000000000000000000000..e4fc884b3aefb3d90a1eb9b43c3b6647b0bdd62c
--- /dev/null
+++ b/PL9fwy3NUQKwY9jl8xw-bWkVZkWcGGyspd/Eut_8KsMs84_postprocess.srt
@@ -0,0 +1,3480 @@
+1
+00:00:04,860 --> 00:00:08,240
+السلام عليكم ورحمة الله وبركاته طالبات العزيزيات
+
+2
+00:00:08,240 --> 00:00:10,600
+اليوم ان شاء الله راح نشرح محاضرة بعنوان
+
+3
+00:00:10,600 --> 00:00:14,780
+microscope راح نتعرف على ال microscope أجزاءه
+
+4
+00:00:14,780 --> 00:00:20,080
+وظائف هذه الأجزاء وأيضا أنواع ال microscope طريقة
+
+5
+00:00:20,080 --> 00:00:25,120
+استخدام ال microscope وطريقة العناية به ومبدأ عمل
+
+6
+00:00:25,120 --> 00:00:29,470
+ال microscopeففي البداية ال microscope هو عبارة عن
+
+7
+00:00:29,470 --> 00:00:34,470
+is an important device that enable us to visualize
+
+8
+00:00:34,470 --> 00:00:39,430
+minute objects هو عبارة عن أداء مهمة important
+
+9
+00:00:39,430 --> 00:00:46,550
+device that enable us تمكننا من رؤية أو إيضاح ال
+
+10
+00:00:46,550 --> 00:00:51,630
+minute objects الأجزاء أو المكونات أو العناصر
+
+11
+00:00:51,630 --> 00:00:55,490
+الدقيقة جدا في العينة سواء كانت هذه العناصر
+
+12
+00:00:55,490 --> 00:01:01,730
+animateحية أو in animate غير حية وهي ال objects
+
+13
+00:01:01,730 --> 00:01:07,190
+طبعا لا نتمكن من رؤيتها بالعين المجردة لذلك نستعين
+
+14
+00:01:07,190 --> 00:01:10,470
+بال microscope نراها تحت ال microscope that you
+
+15
+00:01:10,470 --> 00:01:14,730
+can't be seen by our naked eye لذلك لا يمكن رؤيتها
+
+16
+00:01:14,730 --> 00:01:19,510
+بالعين المجردةنراها تحت ال microscope إذا هو
+
+17
+00:01:19,510 --> 00:01:22,770
+الأداة اللي بتمكننا من رؤية ال minor objects سواء
+
+18
+00:01:22,770 --> 00:01:25,530
+كانت هذه ال objects حية أو غير حية سواء كانت
+
+19
+00:01:25,530 --> 00:01:29,510
+المكونات أو العناصر أو الأجزاء المراد إيضاحها حية
+
+20
+00:01:29,510 --> 00:01:34,410
+أو غير حية بداية هذا هو ال microscope مما يتكون ال
+
+21
+00:01:34,410 --> 00:01:39,410
+microscope أول إشي يتكون من ocular lenses تمام؟
+
+22
+00:01:39,410 --> 00:01:43,050
+ocular lenses أو eyepiece نفس المعنى سواء حكينا
+
+23
+00:01:43,050 --> 00:01:50,110
+eyepiece أوocular lenses نفس المعنى طيب و أيضا
+
+24
+00:01:50,110 --> 00:01:56,170
+مكون من ال tube و هذه ال tube هي اللي بتنقل الصورة
+
+25
+00:01:56,170 --> 00:01:59,990
+طبعا اللي بتاخدها العدسات الشيئية و بتوديها على
+
+26
+00:01:59,990 --> 00:02:06,730
+العدسات العينية عشان يتم تكبيرها تمام؟ و المكون من
+
+27
+00:02:06,730 --> 00:02:15,470
+ذراع arm هذا c arm طيب مكون أيضا من base قاعدةمكون
+
+28
+00:02:15,470 --> 00:02:19,310
+من course adjustment العجل هذا الكبير اسمه course
+
+29
+00:02:19,310 --> 00:02:22,950
+adjustment و العجل الصغير fine adjustment طبعا
+
+30
+00:02:22,950 --> 00:02:26,090
+موجودين هايهم course adjustment و fine adjustment
+
+31
+00:02:26,090 --> 00:02:32,190
+طيب مكون من light source و iris diaphragm و
+
+32
+00:02:32,190 --> 00:02:35,910
+condenser و هذه stage هذه المنصة أو الطاولة اللي
+
+33
+00:02:35,910 --> 00:02:40,470
+بنحط عليها العينة العينة يتم تثبيتها من خلال ملاقت
+
+34
+00:02:40,470 --> 00:02:46,600
+بنسميها clips أو stage clipsتمام؟ هذه الـ
+
+35
+00:02:46,600 --> 00:02:49,180
+objective lenses اللي هي العدسات الشيئية اللي ..
+
+36
+00:02:49,180 --> 00:02:52,600
+اللي هي بتكبر الشيء المراد فحصه اللي .. اللي ..
+
+37
+00:02:52,600 --> 00:02:57,860
+اللي بنكون حاطينه في شريحة على ال stage هنا تمام؟
+
+38
+00:02:57,860 --> 00:03:02,080
+فإذا هذه هي مكونات ال .. ال microscope راح نتحدث
+
+39
+00:03:02,080 --> 00:03:06,220
+عنها بالتفصيل بداية خلونا لما بنحكي عن ال major
+
+40
+00:03:06,220 --> 00:03:08,940
+parts of microscope الأجزاء الأساسية في ال
+
+41
+00:03:08,940 --> 00:03:13,360
+microscope نقسمهم إلى أجزاءأول إشي خلونا نحكي عن
+
+42
+00:03:13,360 --> 00:03:16,720
+الهيكل ال framework of microscope الشكل الخارجي لل
+
+43
+00:03:16,720 --> 00:03:21,280
+microscope مما يتكون .. يتكون من تلت قطع أو تلت
+
+44
+00:03:21,280 --> 00:03:26,000
+مناطق تمام؟ الشكل الخارجي لل microscope أول إشي ال
+
+45
+00:03:26,000 --> 00:03:30,480
+arm هادي اللي انتوا شايفينها هاي ال arm هي اللي
+
+46
+00:03:30,480 --> 00:03:36,370
+بتوصل ال .. ال .. ال ..الجسم ال microscope ببعضه
+
+47
+00:03:36,370 --> 00:03:42,550
+إذا هي اللي بتعمل attachment أو يصال أو ربط ما بين
+
+48
+00:03:42,550 --> 00:03:49,500
+اللي هو العدسات و ال stage و القاعدة، تمام؟فإذا هي
+
+49
+00:03:49,500 --> 00:03:53,380
+الجزء الأساسي في أو اللي هي عماد ال microscope
+
+50
+00:03:53,380 --> 00:03:58,260
+اللي عليها يتم إيصال الأجزاء كلها ببعضها البعض ال
+
+51
+00:03:58,260 --> 00:04:02,580
+stage هو عبارة عن ال table الطاولة هاي أو المنصة
+
+52
+00:04:02,580 --> 00:04:05,960
+هاي اللي عليها إحنا بنحط ال slide و ال slide بيكون
+
+53
+00:04:05,960 --> 00:04:10,320
+عليها الشريحة أو هي ال slide هي الشريحة اللي بيكون
+
+54
+00:04:10,320 --> 00:04:15,780
+عليها العينة المراد فحصهاFoot or base القدم أو
+
+55
+00:04:15,780 --> 00:04:20,120
+القاعدة هي القاعدة المستطيلة high اللي الجهاز كله
+
+56
+00:04:20,120 --> 00:04:23,760
+rest عليها يرتاح عليها و يجلس عليها و يستند عليها
+
+57
+00:04:23,760 --> 00:04:28,280
+طبعا فإذا لو أنا بدي أقسمه بقسمه إلى تلت مناطق
+
+58
+00:04:29,690 --> 00:04:33,790
+تمام؟ و ال .. ال stage و ال base هذا هو الهيكل
+
+59
+00:04:33,790 --> 00:04:36,670
+الخارجي لل microscope the frame out work أو اللي
+
+60
+00:04:36,670 --> 00:04:41,590
+هي the framework of the microscope طيب نشوف الجهاز
+
+61
+00:04:41,590 --> 00:04:46,670
+او ال .. ال .. ال .. ال system التاني من تركيب ال
+
+62
+00:04:46,670 --> 00:04:50,810
+microscope اللي هو ال focusing system نظام التوضيح
+
+63
+00:04:50,810 --> 00:04:53,830
+في ال microscope احنا حكينا ان ال microscope بيوضح
+
+64
+00:04:53,830 --> 00:04:58,150
+بيكبر بيمكني من رؤية الأجزاء اللي هي لا ترى بالعين
+
+65
+00:04:58,150 --> 00:05:03,300
+المجردةطيب أكيد عنده نظام توضيح ونظام تكبير خاص
+
+66
+00:05:03,300 --> 00:05:08,200
+بعرض الصور بدقة متناهية إذا ال .. ال focusing
+
+67
+00:05:08,200 --> 00:05:12,720
+system هو نظام ال .. ال .. ال .. التوضيح أو
+
+68
+00:05:12,720 --> 00:05:17,810
+التركيز في ال microscopeو هذا النظام يتكون من
+
+69
+00:05:17,810 --> 00:05:23,910
+عجلين تمام two knobs هدول ال two knobs بنسميهم
+
+70
+00:05:23,910 --> 00:05:29,030
+لكبير بنسمي course focusing adjustment و لصغير
+
+71
+00:05:29,030 --> 00:05:33,200
+fine focusing adjustmentللاختصار بنحكي course
+
+72
+00:05:33,200 --> 00:05:38,800
+adjustment و fine adjustment تمام؟ إذا مكون من two
+
+73
+00:05:38,800 --> 00:05:43,600
+knobs هدول هيهم pair of knobs و الكبير course
+
+74
+00:05:43,600 --> 00:05:47,300
+adjustment و الصغير fine adjustment طيب ما هو
+
+75
+00:05:47,300 --> 00:05:49,780
+وظيفة ال course adjustment و ال fine adjustment؟
+
+76
+00:05:49,780 --> 00:05:54,350
+شوفوا معاياال course adjustment خلّيني أرجع على ال
+
+77
+00:05:54,350 --> 00:05:58,870
+microscope هنا ال course هايهم هدول العجلين أو
+
+78
+00:05:58,870 --> 00:06:02,610
+القضيبين هدول بنسميهم course adjustment الكبير و
+
+79
+00:06:02,610 --> 00:06:07,890
+الصغير fine adjustment طيب إيش وظيفة ال course
+
+80
+00:06:07,890 --> 00:06:10,910
+adjustment؟ شوفوا معايا أنا لما أحط الشريحة هنا
+
+81
+00:06:10,910 --> 00:06:15,810
+اللي عليها العينةمحتاجة أقرب المسافة ما بين
+
+82
+00:06:15,810 --> 00:06:19,770
+العدسات الشيئية التي ترى الشيء تمام أو تكبر الشيء
+
+83
+00:06:19,770 --> 00:06:23,930
+المراد فحصه وما بين الطاولة أو المنصة أو ال stage
+
+84
+00:06:23,930 --> 00:06:28,310
+اللي موجود عليه هذه العينة تمام؟ محتاجة أن المسافة
+
+85
+00:06:28,310 --> 00:06:33,790
+تكون قريبة عشان يتم تكبير ال .. ال .. ال ..العنصر
+
+86
+00:06:33,790 --> 00:06:40,430
+و أيضا يتم عرضه بإيضاح، يتم عرضه بإيضاح طيب، إذا
+
+87
+00:06:40,430 --> 00:06:45,090
+لابد من أنه يا إما العدسات الشيئية تقترب من ال
+
+88
+00:06:45,090 --> 00:06:50,130
+stage أو ال stage يقترب من العدسات الشيئية، تمام؟
+
+89
+00:06:51,210 --> 00:06:56,270
+فبتحريك هذا ال knobs اللي هو ال .. ال .. ال course
+
+90
+00:06:56,270 --> 00:07:01,330
+adjustment تمام؟ بتحريك هذا ال knob يتم إما تقريب
+
+91
+00:07:01,330 --> 00:07:04,850
+العدسات الشيئية من ال stage أو تقريب ال stage من
+
+92
+00:07:04,850 --> 00:07:07,880
+العدسات الشيئيةتمام هتشوفوا في مايكروسكوبات
+
+93
+00:07:07,880 --> 00:07:11,780
+العدسات الشيئية هي اللي بتتحرك وفي مايكروسكوبات لأ
+
+94
+00:07:11,780 --> 00:07:14,220
+ال stage اللي بتتحرك انتوا هتشتغلوا تقريبا معظم
+
+95
+00:07:14,220 --> 00:07:17,440
+مايكروسكوبات اللي عندنا بالقسم هو ال stage هو اللي
+
+96
+00:07:17,440 --> 00:07:23,080
+بيتحرك بيصعد بالقرب من العدسات الشيئيةتقترب
+
+97
+00:07:23,080 --> 00:07:26,940
+العدسات الشيئية من الشريحة اللى موجودة عليها
+
+98
+00:07:26,940 --> 00:07:31,620
+العينة المراد فحصها وبالتالي وبالتالي بتكون
+
+99
+00:07:31,620 --> 00:07:36,220
+العدسات الشيئية قريبة من ال objects تمام to be
+
+100
+00:07:36,220 --> 00:07:40,120
+visualized اللى احنا بدنا نوضحه فبتلتقط الى صورة
+
+101
+00:07:40,120 --> 00:07:44,940
+مكبرة وهذه الصورة المكبرة تنتقل عبر ال tube او
+
+102
+00:07:44,940 --> 00:07:50,550
+القناع اللى هنا الى العدساتال .. العينية يتم
+
+103
+00:07:50,550 --> 00:07:55,210
+تكبيره مرة أخرى وإذا احنا بناخد ال .. الصورة
+
+104
+00:07:55,210 --> 00:07:58,950
+النهائية بتكون مكبرة مرتين مرة بقوة تكبير العدسات
+
+105
+00:07:58,950 --> 00:08:02,710
+الشيئية أو العدسة الشيئية المستخدمة و .. اللي هي
+
+106
+00:08:02,710 --> 00:08:06,470
+بتكون صورة أولية و الصورة الأولية هدي تنتقل إلى
+
+107
+00:08:06,470 --> 00:08:10,990
+العدسات العينية ويتم تكبيرها مرة أخرى باستخدام
+
+108
+00:08:10,990 --> 00:08:17,430
+العدسات العينية تمام؟فبالتالي إذا لحتى الآن إحنا
+
+109
+00:08:17,430 --> 00:08:20,810
+بنعرف إنه ال course adjustment هو اللي مسؤول عن
+
+110
+00:08:20,810 --> 00:08:25,410
+تقريب ال stage من العدسات الشيئية أو تقريب العدسات
+
+111
+00:08:25,410 --> 00:08:29,050
+الشيئية من ال stage ليتم التقاط الصورة الأولية،
+
+112
+00:08:29,050 --> 00:08:32,730
+إنه يعني العدسات الشيئية تكبرلي ال .. ال .. ال
+
+113
+00:08:32,730 --> 00:08:37,730
+objects تمام؟ أو ال .. اللي هي محتويات العينةطيب
+
+114
+00:08:37,730 --> 00:08:42,970
+ال fine adjustment هاي طب .. لإش وظيفته؟ كل خلية
+
+115
+00:08:42,970 --> 00:08:48,630
+.. كل كائن حي .. كل molecule .. كل مكون object
+
+116
+00:08:48,630 --> 00:08:53,550
+عنصر بإحنا بنتحصه بيكون إله أبعاد هو ما بيكون مصمم
+
+117
+00:08:53,550 --> 00:08:56,110
+يعني هيك نايم على ال slide و ما .. لأ بيكون إله
+
+118
+00:08:56,110 --> 00:09:02,190
+طولة عرض ارتفاع تمام؟ فال .. ال .. ال fine
+
+119
+00:09:02,190 --> 00:09:06,760
+adjustment .. ال fine adjustment تمام؟هو عبارة عن
+
+120
+00:09:06,760 --> 00:09:11,960
+العجل اللي بيتحكم أيضا ب .. بقرب العدسات الشيئية
+
+121
+00:09:11,960 --> 00:09:16,660
+من ال .. ال .. ال object ولكن كيف بيتم .. يعني أو
+
+122
+00:09:16,660 --> 00:09:24,750
+كيف بيتم تحكمه؟هو بيتحكم بعمق الصورة يتحكم بعمق
+
+123
+00:09:24,750 --> 00:09:28,150
+الصورة يعني ممكن يعرضلي تفاصيل مثلا هنا أقل أجبت
+
+124
+00:09:28,150 --> 00:09:32,070
+خلية بدي أشوفها خلية animal cell خلية حيوانية
+
+125
+00:09:32,070 --> 00:09:36,730
+تمام؟ هاي الخلية الحيوانية في في .. هي مش .. يعني
+
+126
+00:09:36,730 --> 00:09:41,850
+إيه؟ هي إلها أبعاد وإلها طول عرض ارتفاع تمام؟ طيب
+
+127
+00:09:41,850 --> 00:09:45,030
+و هاي الخلية في داخلها في عندي مثلا endoplasmic
+
+128
+00:09:45,030 --> 00:09:48,860
+reticulum شبكة endoplasmiaفيها geology apparatus،
+
+129
+00:09:48,860 --> 00:09:52,420
+جهاز geology، فيها نواة، فيها ريبوزومات، فيها
+
+130
+00:09:52,420 --> 00:09:56,760
+سيتوبلازم، تمام؟ هلأ، لو أنا أشوفها أول مرة، لما
+
+131
+00:09:56,760 --> 00:10:00,680
+أحطها على ال slide، و أقرب باستخدام ال course
+
+132
+00:10:00,680 --> 00:10:04,380
+adjustment، أقرب ال .. الاستاج من العدسة الشيئية،
+
+133
+00:10:04,380 --> 00:10:08,700
+و أشوفها، ممكن ما أشوف كل تفاصيل الصورة، يعني ممكن
+
+134
+00:10:08,700 --> 00:10:12,600
+بس تظهرلي ال membrane تبع الخلية، و تظهرلي النواة
+
+135
+00:10:12,600 --> 00:10:19,110
+فقط، لا غيرتمام؟ تظهر لي نواة الخلية فقط طيب لو
+
+136
+00:10:19,110 --> 00:10:22,670
+أنا بدي أشوف تفاصيل أكتر و هي مش موجودة .. يعني
+
+137
+00:10:22,670 --> 00:10:25,070
+هذه التفاصيل ممكن يكون جولجي مش موجودة على نفس
+
+138
+00:10:25,070 --> 00:10:29,350
+العمق تبع ال .. ال .. النوا ف .. او ال endoplasmic
+
+139
+00:10:29,350 --> 00:10:33,490
+reticulum مثلا مش موجودة على عمق جولجي تمام؟ لإنه
+
+140
+00:10:33,490 --> 00:10:38,160
+احنا حكينا الخلية إلها أبعادف ال .. ال fine
+
+141
+00:10:38,160 --> 00:10:43,320
+adjustment هو اللي بيتحكم بعمق الصورة يعني ممكن
+
+142
+00:10:43,320 --> 00:10:46,640
+إذا كان أنا الصورة الأولية كان فقط مبين فيها
+
+143
+00:10:46,640 --> 00:10:51,140
+membrane و نواة ال fine adjustment لما أحركه تمام
+
+144
+00:10:51,140 --> 00:10:56,110
+لما ألعبتيممكن يبينلي جولجي يختفي جولجي يتبين
+
+145
+00:10:56,110 --> 00:10:59,670
+endoplasmic reticulum إذا هو بيبحث على أبعاد أو
+
+146
+00:10:59,670 --> 00:11:04,310
+على أعماق مختلفة من الصورة ذات نفسها، تمام؟ ليش؟
+
+147
+00:11:04,310 --> 00:11:08,230
+لإن مكونات ال object المراد فحصه أحيانا بتكون
+
+148
+00:11:08,230 --> 00:11:11,290
+الشكلها على نفس المسافة لإن ال object هذا إله
+
+149
+00:11:11,290 --> 00:11:15,970
+أبعاد، تمام؟ إله أبعادفإذا ال fine adjustment
+
+150
+00:11:15,970 --> 00:11:20,910
+بيورجيني ال details تمام بيورجيني ال details هذا
+
+151
+00:11:20,910 --> 00:11:25,430
+ال fine adjustment بستخدمه خصوصا لو كنت أنا بفحص
+
+152
+00:11:25,430 --> 00:11:30,970
+العينة على ال high power objective lens علىال ..
+
+153
+00:11:30,970 --> 00:11:36,770
+العدسات الشيئية اللي قوة تكبيرها كبيرة نوع عامة
+
+154
+00:11:36,770 --> 00:11:41,710
+طيب، Condenser Adjustment فيه عندي أيضا نب قديب أو
+
+155
+00:11:41,710 --> 00:11:46,110
+عجل هنا هذا بسميه Condenser Adjustment إيش وظيفة
+
+156
+00:11:46,110 --> 00:11:49,790
+ال Condenser Adjustment؟ هو بيتحكم ه .. this moves
+
+157
+00:11:49,790 --> 00:11:53,850
+the condenser up or down بيحرك ال condenser إلى
+
+158
+00:11:53,850 --> 00:11:58,490
+أعلى و إلى أسفل، تمام؟ بيحرك إلى أعلى و إلى أسفل
+
+159
+00:11:59,380 --> 00:12:03,900
+فإذا خلّينا بس نرجع نشوف كيف بيتحرك هذا هو ال
+
+160
+00:12:03,900 --> 00:12:06,920
+condenser اللي هنا اللي هنا بنسميه condenser تحت
+
+161
+00:12:06,920 --> 00:12:10,580
+ال stage مباشرة وهي هذه عدسته اللي مبينة من فتحة
+
+162
+00:12:10,580 --> 00:12:15,560
+ال stage طيب إيش وظيفة ال condenser؟ هو بياخد
+
+163
+00:12:15,560 --> 00:12:18,000
+الضوء من ال light source هنا في عندي light source
+
+164
+00:12:18,000 --> 00:12:22,220
+في عندي لمبة بتعطيني ضوءبيستلم الضوء و من اسمه
+
+165
+00:12:22,220 --> 00:12:25,400
+condenser يكثف الضوء يجمعه علشان اللي جه الضوء
+
+166
+00:12:25,400 --> 00:12:29,400
+قادر يخترق العينة و يصل الى العدسة الشيئية فهنا
+
+167
+00:12:29,400 --> 00:12:34,980
+بيتم وصول صورة واضحة جدا الى العدسة الشيئية تمام؟
+
+168
+00:12:35,610 --> 00:12:42,390
+لإن الضوء تم تكثيفه واختراقه في ال .. في العينة
+
+169
+00:12:42,390 --> 00:12:47,050
+هلأ إحنا مثلا إيش مبدأ الرؤية طبعتنا؟ الأشياء ..
+
+170
+00:12:47,050 --> 00:12:50,510
+الضوء يسقط على الأشياء ينعكس إلى .. إلى عيوننا
+
+171
+00:12:50,510 --> 00:12:55,310
+فنرى الأشياء، صح؟ بدليل إنه بالعتمة إحنا ما بنشوف
+
+172
+00:12:55,990 --> 00:13:00,230
+لأنه مافيش ضوء يسقط على الأشياء وينعكس على عيوننا
+
+173
+00:13:00,230 --> 00:13:04,390
+فبالتالي ما .. ما بنشوف يعني بتكون الأشياء يعني
+
+174
+00:13:04,390 --> 00:13:13,450
+غير مرئية طبعا طيب فبالتالي اللي لازم أنه يكون في
+
+175
+00:13:13,450 --> 00:13:16,990
+عندي تجميع للضوء لحتى يسقط أو يخترق العين على اللي
+
+176
+00:13:16,990 --> 00:13:21,730
+يتم رؤية حتى العدسة الشيئية هي يمكنها رؤيتها طبعا
+
+177
+00:13:21,730 --> 00:13:28,870
+و تكبيرها وصورة أولية منهاطيب ال .. ال knob هذا
+
+178
+00:13:28,870 --> 00:13:31,590
+القضيب اللى عالجنب هنا من هنا اللى بنسميه
+
+179
+00:13:31,590 --> 00:13:38,390
+condenser adjustment هو بيتحكم ببعد او تحريك هذا
+
+180
+00:13:38,390 --> 00:13:42,930
+ال condenser up او down بنزله لتحت فبيبعد هذا ال
+
+181
+00:13:42,930 --> 00:13:47,070
+condenser عن ال stage فبصير كمية ضوء قليلة تخترق
+
+182
+00:13:47,070 --> 00:13:51,270
+العينة هذا يتناسب مع العدسات اللى قوة تكبيرها
+
+183
+00:13:51,270 --> 00:13:57,190
+قليلةو ممكن إن أنا أقربه من العدسة أحركه للأعلى
+
+184
+00:13:57,190 --> 00:14:01,230
+فيقترب أكتر من العينة فيصير يجمع لي كمية ضوء أكبر
+
+185
+00:14:01,230 --> 00:14:04,370
+لتختارق العينة وهذا يتناسب مع العدسات اللي قوة
+
+186
+00:14:04,370 --> 00:14:08,630
+تكبيرها كبيرة تمام؟ لإن مع العدسات اللي قوة
+
+187
+00:14:08,630 --> 00:14:13,090
+تكبيرها كبيرة احنا محتاجين كمية ضوء أكبر عشان
+
+188
+00:14:13,090 --> 00:14:19,180
+العدسة تتمكن من التقاط صورة مكبرة واضحةتمام؟ و مع
+
+189
+00:14:19,180 --> 00:14:24,060
+العدسات اللي قوة تكبيرها صغيرة محتاجين كمية ضوء
+
+190
+00:14:24,060 --> 00:14:27,100
+أقل تمام؟ للإضاءة
+
+191
+00:14:29,530 --> 00:14:33,230
+طيب، إذا صيرنا بنعرف إيش يعني course adjustment،
+
+192
+00:14:33,230 --> 00:14:37,970
+إيش يعني fine adjustment، وإيش معنى ال condenser
+
+193
+00:14:37,970 --> 00:14:43,470
+adjustment طيب، في مكون آخر أيضا يتحكم بكمية الضوء
+
+194
+00:14:43,470 --> 00:14:47,410
+اللي مارة للعينة، اللي هو عبارة عن ال iris
+
+195
+00:14:47,410 --> 00:14:52,110
+diaphragm، تمام؟ ال iris diaphragmخلينا نشوف إيش
+
+196
+00:14:52,110 --> 00:14:55,810
+هو ال iris diaphragm؟ ال iris diaphragm هو عبارة
+
+197
+00:14:55,810 --> 00:14:59,450
+عن اللسان المعدني هذا اللي مطرود هذا اللسان
+
+198
+00:14:59,450 --> 00:15:02,750
+المعدني هذا هو عبارة عن ال iris diaphragm اللي
+
+199
+00:15:02,750 --> 00:15:05,850
+أنتوا شايفينه طب هذا إيش وظيفته؟ شوفوا معايا
+
+200
+00:15:06,520 --> 00:15:10,340
+الcondenser موجود تحته القرص diaphragm، diaphragm
+
+201
+00:15:10,340 --> 00:15:16,220
+يعني حجاب أو يعني إشي .. يعني غطاء حاجز، هذا معناه
+
+202
+00:15:16,220 --> 00:15:21,780
+diaphragm طيب هذا ال .. ال .. القرص تبعه، القرص
+
+203
+00:15:21,780 --> 00:15:27,640
+diaphragm بيتحرك يمين و يسار و مع حركته بيسكر
+
+204
+00:15:27,640 --> 00:15:33,610
+الحجاب و بي .. و بيفتحطبعا إذا أغلق الحجاب إذا
+
+205
+00:15:33,610 --> 00:15:37,150
+كمية الضوء اللى واصل على الcondenser هتكون قليلة
+
+206
+00:15:37,150 --> 00:15:41,450
+فبالتالي كمية الضوء اللى تخترق العينة قليلة و هذا
+
+207
+00:15:41,450 --> 00:15:47,430
+حكينا أشماله أيضا يناسب من .. يناسب العدسات اللى
+
+208
+00:15:47,430 --> 00:15:54,270
+قوة تكبيرها قليلةوإذا تم فتح هذا القرص إذا الحجاب
+
+209
+00:15:54,270 --> 00:15:57,610
+مفتوح إذا كمية الضوء اللي بتعبر إلى الcondenser
+
+210
+00:15:57,610 --> 00:16:02,590
+كتيرة وكمية الضوء اللي يخترق العينة كبيرة فهذا
+
+211
+00:16:02,590 --> 00:16:08,260
+يناسب لعدسات اللي قوة تكبيرها كبيرةتمام؟ إذا في
+
+212
+00:16:08,260 --> 00:16:12,520
+عندي نوعين من التحكم في كمية الضوء اللي مارة
+
+213
+00:16:12,520 --> 00:16:18,960
+للعينة أول طريقة التحكم اللي هي عبارة عن ال .. ال
+
+214
+00:16:18,960 --> 00:16:23,120
+.. الـ Condenser Adjustment وطريقة التحكم الثانية
+
+215
+00:16:23,120 --> 00:16:27,180
+هي القرص الأكثر شيوعا واستخداما هي القرص يعني أنه
+
+216
+00:16:27,180 --> 00:16:30,680
+احنا بنستخدمها أكثر من أنه احنا نرفع وننزل
+
+217
+00:16:30,680 --> 00:16:34,150
+Condenserولكن يمكن استخدام أيضًا ال .. ال .. ال ..
+
+218
+00:16:34,150 --> 00:16:38,350
+الـ Condenser Adjustment Knob علشان نتحكم بكمية
+
+219
+00:16:38,350 --> 00:16:42,690
+الضوء اللي مارة إلى ال .. تخترق العينة وتمر إلى
+
+220
+00:16:42,690 --> 00:16:49,630
+العدسات الشيية تمام؟فهذا هو ال .. ال .. طريقة
+
+221
+00:16:49,630 --> 00:16:54,310
+التحكم course condenser adjustment وعندي كمان اللي
+
+222
+00:16:54,310 --> 00:16:56,830
+هو ال earth diaphragm هاي هنا بيحكيلي the
+
+223
+00:16:56,830 --> 00:17:00,950
+principal purpose of the condenser is to condense
+
+224
+00:17:00,950 --> 00:17:04,690
+the light وظيفته ال condenser انه يكسر في الضوء
+
+225
+00:17:04,690 --> 00:17:07,870
+اللي .. و .. اللي يخترق العينة و اللي احنا
+
+226
+00:17:07,870 --> 00:17:12,430
+محتاجينه لاش لل visualization للاضاح و ال .. و ال
+
+227
+00:17:12,430 --> 00:17:18,260
+.. و التكبير و انه التقاط صورة واضحةطيب، حكينا
+
+228
+00:17:18,260 --> 00:17:22,920
+لحتى الآن عن ال frame، الهيكل للميكروسكوب، حكينا
+
+229
+00:17:22,920 --> 00:17:27,600
+عن جهاز التركيز أو نظام التركيز اللي هو عبارة عن
+
+230
+00:17:27,600 --> 00:17:31,580
+course adjustment, fine adjustment, condenser
+
+231
+00:17:31,580 --> 00:17:38,590
+adjustment و ال iris diaphragmو أيضًا راح نحكي عن
+
+232
+00:17:38,590 --> 00:17:41,770
+نظام التكبير، طبعًا؟ خلّيني أحكينا عن نظام
+
+233
+00:17:41,770 --> 00:17:46,230
+التركيز، هنحكي عن نظام التكبير، طبعًا؟ ما هي
+
+234
+00:17:46,230 --> 00:17:50,730
+الأدوات اللي مسؤولة عن تكبير ال object في ال
+
+235
+00:17:50,730 --> 00:17:56,150
+microscope؟ هي ال objective lenses، طبعًا؟ و ال
+
+236
+00:17:56,150 --> 00:17:59,130
+ocular lenses، إذا العدسات اللي موجودة في ال
+
+237
+00:17:59,130 --> 00:18:04,480
+microscope هي اللي مسؤولة عن التكبيرفي عندى عدسات
+
+238
+00:18:04,480 --> 00:18:09,000
+objective lenses تمام ومن اسمها objective ليش
+
+239
+00:18:09,000 --> 00:18:12,120
+تسمينها objective لإنها قريبة من ال object المراد
+
+240
+00:18:12,120 --> 00:18:17,700
+فحصه تمام قريبة من ال object المراد فحصه لذلك تسمى
+
+241
+00:18:17,700 --> 00:18:23,760
+objectiveطيب هذه العدسات أربعة، كل عدسة إلها قوة
+
+242
+00:18:23,760 --> 00:18:29,160
+تكبير مختلفة، هي أنا عندي 4x يعني ال .. ال object
+
+243
+00:18:29,160 --> 00:18:34,760
+بيكون مكبر أربع مرات، تمام؟ ال .. في .. في الصورة
+
+244
+00:18:34,760 --> 00:18:38,100
+الأولية اللي يتم التقاطها
+
+245
+00:18:39,060 --> 00:18:44,300
+تمام؟ هنا ال object بيتم تكبيره 10x يعني عشر مرات
+
+246
+00:18:44,300 --> 00:18:49,180
+في الصورة الأولية، هنا عندي 40x وهنا 100x تمام؟
+
+247
+00:18:49,180 --> 00:18:54,300
+لما أحكيلك low power objective تمام؟ يعني قوة
+
+248
+00:18:54,300 --> 00:18:56,980
+تكبير قليلة من ال objective معناها بدك تستخدم
+
+249
+00:18:56,980 --> 00:19:01,210
+العدسة أربعة أو عشرةلمّا أحكيلك high power
+
+250
+00:19:01,210 --> 00:19:05,130
+objective يعني بدك تستخدم العدسة أربعين، لما
+
+251
+00:19:05,130 --> 00:19:09,190
+أحكيلك oil immersion objective يعني العدسة مية،
+
+252
+00:19:09,190 --> 00:19:13,670
+تمام؟ إذا إما بحبنعرفهم بقوة التكبير تبعتهم أو
+
+253
+00:19:13,670 --> 00:19:18,370
+بpower تبعهم، هذه low power, high power, oil
+
+254
+00:19:18,370 --> 00:19:18,850
+immersion
+
+255
+00:19:21,980 --> 00:19:27,360
+طيب إذا و العدسات العينية هي ال upper optical
+
+256
+00:19:27,360 --> 00:19:30,260
+component اللي هي الجزء اللي .. اللي إحنا بنشوف
+
+257
+00:19:30,260 --> 00:19:35,520
+فيه و بيكون موجود في أعلى ال microscope و طبعا
+
+258
+00:19:35,520 --> 00:19:39,960
+وظيفة حكينا إنه العدسات الشيئية بتلتقط صورة أولية
+
+259
+00:19:39,960 --> 00:19:46,880
+مكبرة إما 4X أو 10X أو 40X أو 100Xتمام؟ هذه الصورة
+
+260
+00:19:46,880 --> 00:19:54,070
+الأولية تمام بتنتقل عبر ال tube إلى ال .. ال ..الـ
+
+261
+00:19:54,070 --> 00:19:58,190
+ocular lenses فهي ال ocular lenses وظيفتها انها
+
+262
+00:19:58,190 --> 00:20:03,030
+magnify the primary image بتكبر الصورة الأولية مرة
+
+263
+00:20:03,030 --> 00:20:06,810
+أخرى تمام؟ و هذه العدسات موجودة ممكن يكون قوة
+
+264
+00:20:06,810 --> 00:20:13,290
+تكبيرها 4x, 6x, 7x او 10 او حتى 15 او 20x تمام؟
+
+265
+00:20:13,290 --> 00:20:16,630
+فإذا إلها قوات تكبير مختلفة ال more common use
+
+266
+00:20:16,630 --> 00:20:20,150
+اللي انتوا هتستخدموه مايكروسكوبات ال ocular lenses
+
+267
+00:20:20,150 --> 00:20:26,760
+تبعتها 10xتمام؟ ف .. اذا ان هي بتكبر الصورة
+
+268
+00:20:26,760 --> 00:20:31,840
+المبدئية او الاولية اللى وصلتها من ال objective فى
+
+269
+00:20:31,840 --> 00:20:41,160
+عندى microscope و ال .. طبعا ال .. بنوعين حسب عدد
+
+270
+00:20:41,160 --> 00:20:44,800
+ال eyepiece اللى موجود فيه فى عندى ال microscope
+
+271
+00:20:44,800 --> 00:20:48,960
+اللى بيكون فيه one ocular lenses او one eyepiece
+
+272
+00:20:49,460 --> 00:20:57,700
+بنسمي mono-ocular طبعا إذا كان في عدستين عينية two
+
+273
+00:20:57,700 --> 00:21:02,880
+او اوكيولار lenses بنسمي bi-ocular طبعا إذا ال
+
+274
+00:21:02,880 --> 00:21:06,340
+microscope بنسمي bi-ocular او mono-ocular حسب عدد
+
+275
+00:21:06,340 --> 00:21:10,600
+العدسات العينية اللي موجودة فيه جدير بتذكر انه
+
+276
+00:21:10,600 --> 00:21:14,360
+احكي eyepiece او احكي اوكيولار lenses نفس المعنى
+
+277
+00:21:16,460 --> 00:21:21,920
+طيب حكينا عن جهاز أو نظام التكبير نحكي عن نظام ال
+
+278
+00:21:21,920 --> 00:21:26,500
+illumination الإضاءة و التحكم بالإضاءة أيضا ال
+
+279
+00:21:26,500 --> 00:21:32,720
+condenser و ال iris من ال .. يتبعوا للنظام التوضيح
+
+280
+00:21:32,720 --> 00:21:36,650
+و الإضاءة تمام؟اللي هو احنا حكينا الـ Condenser
+
+281
+00:21:36,650 --> 00:21:40,330
+وظيفته انه بي .. بيجمع الضوء اللي واصله من ال
+
+282
+00:21:40,330 --> 00:21:43,550
+light source تمام؟ و بيخليه يختارق العينة اللي
+
+283
+00:21:43,550 --> 00:21:47,190
+يمكن رؤية الصورة الأولية بوسط ال objective lens
+
+284
+00:21:47,190 --> 00:21:52,070
+تمام؟و ده هي ال .. the condenser عدسة ال condenser
+
+285
+00:21:52,070 --> 00:21:55,710
+هاي اللي بتكون .. حكينا بتكون مبينة من فتحة ال ..
+
+286
+00:21:55,710 --> 00:21:59,150
+ال .. ال stage بتكون تستلم الضوء من وين؟ من ال
+
+287
+00:21:59,150 --> 00:22:02,850
+light source و بتمرره خلال العينة و إلى ال
+
+288
+00:22:02,850 --> 00:22:05,890
+objective هذا ال earth ده في الرقم شايفينه بيكون
+
+289
+00:22:05,890 --> 00:22:07,230
+موجود تحت ال condenser
+
+290
+00:22:12,640 --> 00:22:19,000
+طبعا مع حركة القرص هذا زي حجاب بيسكر و بيفتح، طبعا
+
+291
+00:22:19,000 --> 00:22:22,720
+بيسكر و بيفتح، اذا بتحكم بكمية الضوء اللي مارة من
+
+292
+00:22:22,720 --> 00:22:26,140
+ال light source إلى الcondenserهين زي ما انتوا
+
+293
+00:22:26,140 --> 00:22:29,460
+شايفين هي هذا القرص مفتوح على الآخر، هذا مغلق
+
+294
+00:22:29,460 --> 00:22:33,580
+شوية، هذا مغلق أكتر، فإذا حسب حركة ال .. ال ..
+
+295
+00:22:33,580 --> 00:22:37,480
+القرص diaphragm هذا بي .. بي .. بيسكرلي ال
+
+296
+00:22:37,480 --> 00:22:42,130
+diaphragm أو بيفتحهمتمام؟ إذا the iris is
+
+297
+00:22:42,130 --> 00:22:46,230
+mechanical device mounted underneath the condenser
+
+298
+00:22:46,230 --> 00:22:50,930
+موجودة تحت الcondenser and controls the amount of
+
+299
+00:22:50,930 --> 00:22:55,470
+light بتتحكم بكمية الضوء اللي فايت على الcondenser
+
+300
+00:22:55,470 --> 00:22:58,730
+من وين؟ من ال light source تمام؟ من ال light
+
+301
+00:22:58,730 --> 00:23:03,710
+source يوجد أيضا أحيانا بيكون mirror وين بتكون ال
+
+302
+00:23:03,710 --> 00:23:08,020
+mirror هادي؟نرجع لصورة ال microscope بيكون هنا
+
+303
+00:23:08,020 --> 00:23:12,040
+أحيانا فوق ال light source بيكون فيه مراية ليش؟
+
+304
+00:23:12,040 --> 00:23:15,180
+المراية هذه وظيفتها أنها تاخد الضوء اللي جاي من ال
+
+305
+00:23:15,180 --> 00:23:21,000
+light source و تعكسه لحتى يتم انعكاسه إلى ال
+
+306
+00:23:21,000 --> 00:23:25,020
+condenser تمام؟ لينعكس إلى ال condenser بس يعني
+
+307
+00:23:25,020 --> 00:23:32,720
+لتعزيز كفاءة استخدام الضوء فقط لا غير أحيانا في ..
+
+308
+00:23:34,540 --> 00:23:37,880
+مايكروسكوبات ما بتعمل على .. يعني مافيها لمبة
+
+309
+00:23:37,880 --> 00:23:41,100
+بتعمل على ضوء النهار أو ضوء المنطقة المحيطة فيها
+
+310
+00:23:41,100 --> 00:23:44,720
+فلازم يكون في عندي عدسة جامع على الضوء و .. لحتى
+
+311
+00:23:44,720 --> 00:23:47,360
+تجمع الضوء اللي موجود في المنطقة اللي موجود فيها
+
+312
+00:23:47,360 --> 00:23:51,880
+المايكروسكوب و توجهه باتجاه الـ Condenserتمام؟
+
+313
+00:23:51,880 --> 00:23:57,120
+فإذا هذه فائدة الـ mirror الـ mirror بتكون تحت الـ
+
+314
+00:23:57,120 --> 00:24:02,260
+condenser و تحت القرص أيضا و فوق ال light source
+
+315
+00:24:02,260 --> 00:24:06,440
+مباشرة و إيش وظيفتها reflect the beam of light
+
+316
+00:24:06,440 --> 00:24:10,560
+تعكس اللي هو الضوء إلى .. باتجاه ال condenser into
+
+317
+00:24:10,560 --> 00:24:16,580
+the condenserماشي و سواء كان هذا الضوء جاي من ال
+
+318
+00:24:16,580 --> 00:24:19,520
+daylight أو من ال electrical light من الضوء
+
+319
+00:24:19,520 --> 00:24:21,860
+الكهربائي أو اللمبة اللي موجودة في ال microscope
+
+320
+00:24:23,750 --> 00:24:28,950
+طيب إذا مصادر الإضاءة daylight اللي هو يعني ضوء
+
+321
+00:24:28,950 --> 00:24:34,330
+المحيط بالجهاز يعني إن أكتفي بالضوء للنهار مثلا و
+
+322
+00:24:34,330 --> 00:24:40,110
+هذا ممكن يكون sufficient يعني كافي في بعض الأشياء
+
+323
+00:24:40,110 --> 00:24:44,750
+ولكن أحيانا بيكون غير كافي إذا أنا بدأ أستخدم قوة
+
+324
+00:24:44,750 --> 00:24:47,650
+تكبير كبيرة زي ال oil immersion اللي هي ال 100x
+
+325
+00:24:47,950 --> 00:24:52,430
+حكينا إنه مع القوة التكبير القليلة، تمام؟ ال
+
+326
+00:24:52,430 --> 00:24:56,070
+objective lenses ما بتكون محتاجة ضوء كتير، لكن مع
+
+327
+00:24:56,070 --> 00:24:59,630
+ال .. ال .. القوة التكبير العالية، ال objective
+
+328
+00:24:59,630 --> 00:25:05,190
+بتكون محتاجة كمية ضوء أكبر، تمام؟ بتكون محتاجة
+
+329
+00:25:05,190 --> 00:25:12,510
+كمية ضوء أكبر ال electric light، تمام؟اللي هو ال
+
+330
+00:25:12,510 --> 00:25:16,830
+.. ال .. ال مصدر الضوء اللمبة بتكون tungsten lamp
+
+331
+00:25:16,830 --> 00:25:20,810
+تمام؟ بتكون tungsten lamp أحيانا ممكن تكون طبعا
+
+332
+00:25:20,810 --> 00:25:25,230
+tungsten lamp أو quartz halogen lamp حسب يعني نوع
+
+333
+00:25:25,230 --> 00:25:28,850
+ال microscope و حسب نوع الإضاءة .. النوع الإضاءة
+
+334
+00:25:28,850 --> 00:25:34,050
+اللي موجودة أو اللي إحنا محتاجينها طبعا في ال
+
+335
+00:25:34,050 --> 00:25:39,590
+microscopeطيب فلاتر أحيانا بيكون أيضا موجود في ال
+
+336
+00:25:39,590 --> 00:25:43,990
+.. ال .. ال .. ال illumination system فلاتر
+
+337
+00:25:47,230 --> 00:25:50,630
+طيب الفيلتر هذا عبارة عن إيش؟ هو عبارة عن قطعة
+
+338
+00:25:50,630 --> 00:25:55,790
+زجاجية ملونة تمام؟ وإيش وظيفته؟ بيقلل شدة الضوء
+
+339
+00:25:55,790 --> 00:25:58,970
+اللي مع ال rubber دو كمان بيكون موجود الفيلتر
+
+340
+00:25:58,970 --> 00:26:04,910
+موجود يعني الممكن أحطه هنا هو بيه طبعا هو اللي
+
+341
+00:26:04,910 --> 00:26:09,530
+بتحكي يعني ممكن أنا أركبه أو أشيله تمام؟ يعني أنا
+
+342
+00:26:09,530 --> 00:26:13,110
+بتحكم بإن هو موجود أو غير موجود بس هو بيجي مع ال
+
+343
+00:26:13,110 --> 00:26:17,370
+microscope فلاتر ضوئيةتمام قطعة زجاجية ملونة بتكون
+
+344
+00:26:17,370 --> 00:26:22,170
+موجودة على ال .. ال light source أنا بحطها على ال
+
+345
+00:26:22,170 --> 00:26:25,250
+light source هنا عشان تتحكم بكمية الضوء اللي
+
+346
+00:26:25,250 --> 00:26:29,650
+موجودة ولعل هذه الفكرة طرحناها في محاضرة ال
+
+347
+00:26:29,650 --> 00:26:34,510
+calorie meter وذكرنا انهلو كان في عندي لمبة و أنا
+
+348
+00:26:34,510 --> 00:26:39,850
+حطيت زجاج ملون هذا الزجاج .. الزجاج راح يحجب كمية
+
+349
+00:26:39,850 --> 00:26:46,010
+من الضوء و يمرر ما تبقى، تمام؟ إذا هو بيتحكم بكمية
+
+350
+00:26:46,010 --> 00:26:48,830
+الضوء اللي موجودة أو اللي .. اللي تعبر إلى
+
+351
+00:26:48,830 --> 00:26:52,850
+الcondenser و من ثم إلى ال objectiveطيب ممكن كمان
+
+352
+00:26:52,850 --> 00:26:56,270
+أحيانا في بعض الأجزاء خصوصا الأجزاء اللي غير
+
+353
+00:26:56,270 --> 00:27:02,310
+مسبوغة ممكن يزيد الإيضاح increase resolution تمام؟
+
+354
+00:27:02,310 --> 00:27:06,370
+لإنه في بعض الأشياء اللي احنا بنكون محتاجين نشوفها
+
+355
+00:27:06,530 --> 00:27:12,830
+بتكون هي معتمة، تمام؟ فمش محتاجة ضو كتير يخليها
+
+356
+00:27:12,830 --> 00:27:17,890
+فاتحة و غير مرئية طيب و أيضا كمان وظيفة ال filter
+
+357
+00:27:17,890 --> 00:27:21,190
+انه protect the eye from injury، هلأ احنا مع
+
+358
+00:27:21,190 --> 00:27:28,650
+الاستخدامالمطول لـ Microscope بيتم .. بيصير يعني
+
+359
+00:27:28,650 --> 00:27:32,030
+لمعة الضوء في عينا مؤضية فممكن نستخدم ال filter
+
+360
+00:27:32,030 --> 00:27:35,050
+عشان نحد من كمية الضوء اللي يعني بيخبط في عينا
+
+361
+00:27:35,050 --> 00:27:40,770
+فبالتالي او انه يعني نحافظ على سلامة العين او نكون
+
+362
+00:27:40,770 --> 00:27:45,930
+مرتاحين اكثر و احنا بنشتغل طيب، هيك بنكون عرضنا
+
+363
+00:27:45,930 --> 00:27:53,240
+مكونات ال .. ال .. ال microscopeسردناها كصرد أولي
+
+364
+00:27:53,240 --> 00:27:57,740
+و بعد هيك حكينا إنها مكون من أجزاء الجزء الأول هو
+
+365
+00:27:57,740 --> 00:28:03,260
+الهيكل تلت .. تلت مناطق منطقة ال arm و ال stage و
+
+366
+00:28:03,260 --> 00:28:07,820
+منطقة ال base الجزء الثاني هو منطق اللي هو ال
+
+367
+00:28:07,820 --> 00:28:10,900
+focusing system اللي هو عبارة عن ال course و ال
+
+368
+00:28:10,900 --> 00:28:14,220
+fine adjustment و أيضا ال condenser adjustment و
+
+369
+00:28:14,220 --> 00:28:18,000
+ممكن ييجي معهم القرص حكينا بعدين عن ال elimination
+
+370
+00:28:18,000 --> 00:28:23,560
+system اللي هوالإضاءة ال light source و أيضًا كمان
+
+371
+00:28:23,560 --> 00:28:27,400
+ال condenser و القرص ممكن يكونوا جزء من ال light
+
+372
+00:28:27,400 --> 00:28:32,040
+source او من ال illumination system و ال filter و
+
+373
+00:28:32,040 --> 00:28:36,360
+ال mirror حكينا عن ال magnification system نظام
+
+374
+00:28:36,360 --> 00:28:40,740
+التكبير اللي هو يشمل ال objective lenses و ال
+
+375
+00:28:40,740 --> 00:28:46,070
+ocular lensesطبعا حتى كمان شدة الضوء يا بنات ممكن
+
+376
+00:28:46,070 --> 00:28:50,830
+انا اتحكم فيها من هنا في عندي عجل بيزود الضوء ل ..
+
+377
+00:28:50,830 --> 00:28:56,110
+الضوء اللامبا نفسها بيزود .. بيزودها و بيقلل طبعا
+
+378
+00:28:56,110 --> 00:29:00,610
+و في .. في كبسة أيضا هنا موجودة بتفتح و بتسكر
+
+379
+00:29:00,610 --> 00:29:10,190
+الضوء طيب هذا بالنسبة لال ..مكونات خلينا نشوف مبدأ
+
+380
+00:29:10,190 --> 00:29:13,770
+عمل ال oil immersion ليش احنا بنحكي عن العدسة اللي
+
+381
+00:29:13,770 --> 00:29:22,010
+قوة تكبيرها 100X تمام؟ ليش بنسميها oil immersion؟
+
+382
+00:29:22,010 --> 00:29:26,670
+تمام طيب قبل ان نحكي عن ال oil immersion خلينا
+
+383
+00:29:26,670 --> 00:29:31,100
+نحكي عن ال microscope بشكل عام ايش مبدأ عمله؟ال ..
+
+384
+00:29:31,100 --> 00:29:35,940
+ال microscope بشكل عام هو magnifying instrument،
+
+385
+00:29:35,940 --> 00:29:42,500
+هو عبارة عن جهاز تكبير، تمام؟ و الصورة اللي بتصل
+
+386
+00:29:42,500 --> 00:29:46,400
+ال .. ال .. طبعا ال .. ال .. لأ ال objective
+
+387
+00:29:46,400 --> 00:29:51,320
+lenses بتكون هي عبارة عن ال primary، تمام؟ image
+
+388
+00:29:52,800 --> 00:29:56,920
+بأول حاجة الضوء بيخترق ال object اللى موجودة على
+
+389
+00:29:56,920 --> 00:30:00,760
+ال stage تمام وبيصل الى ال objective ف ال
+
+390
+00:30:00,760 --> 00:30:05,040
+objective هتجمع الضوء هذا و تلتقط صورة الصورة هاي
+
+391
+00:30:05,040 --> 00:30:09,420
+عبارة عن ال primary image مكبرة بقوة تكبير ال
+
+392
+00:30:09,420 --> 00:30:15,300
+objectiveتمام؟ هذه الصورة راح تنتقل إلى عدسة أخرى
+
+393
+00:30:15,300 --> 00:30:18,020
+اللي هي near the eye بالقرب من العين عشان هيك
+
+394
+00:30:18,020 --> 00:30:22,220
+بنسميها ocular lenses أو eyepiece و هذه العدسة
+
+395
+00:30:22,220 --> 00:30:27,140
+العينية راح تكبر ال primary image مرة ثانية تمام؟
+
+396
+00:30:27,140 --> 00:30:32,100
+إذا أنا ممكن أعرف ال total magnification التكبير
+
+397
+00:30:32,100 --> 00:30:37,100
+الكل للميكروسكوب أو للصورة اللي أنا عم بشوفها هو
+
+398
+00:30:37,100 --> 00:30:41,160
+عبارة عن حاصل تكبير ال objective lens ضربحاصل
+
+399
+00:30:41,160 --> 00:30:45,500
+تكبير ال .. ال .. ال ocular lens أو ال Ibs يعني
+
+400
+00:30:45,500 --> 00:30:49,200
+مثلا لو كنت أنا مستخدمة ب .. بشوف خلايا بكتيرية
+
+401
+00:30:49,200 --> 00:30:54,770
+على العدسة عشرة X objective تمام؟وعندي ال IBS
+
+402
+00:30:54,770 --> 00:30:58,750
+حكيتلكم ال most common انها تكون عشرة في قوة تكبير
+
+403
+00:30:58,750 --> 00:31:02,590
+أخرى ولكن احنا عندنا اللي بنستخدمهم عشرة طيب طيب
+
+404
+00:31:02,590 --> 00:31:07,590
+العدسة الشيئية التقطت صورة مكبرة عشر مرات عشرة X
+
+405
+00:31:07,590 --> 00:31:11,970
+ونقلتها إلى ال IBS فكبرتها أيضا عشر مرات اذا
+
+406
+00:31:11,970 --> 00:31:17,290
+التكبير ال total هو عبارة عن عشرة في عشرة ميت X
+
+407
+00:31:17,290 --> 00:31:22,560
+بتكون مكبرة ميتAX 100 مرة تمام هذا ال total
+
+408
+00:31:22,560 --> 00:31:26,020
+magnification طيب لو أنا كنت مستخدمة ال 40X اللي
+
+409
+00:31:26,020 --> 00:31:31,710
+هي ال high power lensفالصورة الأولية هتتكبر 40x
+
+410
+00:31:31,710 --> 00:31:36,050
+ويتنتقل إلى ال Ibs فهتكبرها عشر مرات كمان فالتكبير
+
+411
+00:31:36,050 --> 00:31:40,450
+الكل هيكون 400 تمام؟ في ال oil immersion أو ال
+
+412
+00:31:40,450 --> 00:31:46,130
+100x هتكون الصورة الأولية مكبرة 100 مرة ويتنتقل
+
+413
+00:31:46,130 --> 00:31:50,330
+إلى ال Ibs فتكبرها عشر مرات كمان فبصير في عندي
+
+414
+00:31:50,330 --> 00:32:00,110
+التكبير الكل ألف x تمام؟ ألف xطيب إذا الـ total
+
+415
+00:32:00,110 --> 00:32:04,730
+magnification هو عبارة عن ال .. ال magnification
+
+416
+00:32:04,730 --> 00:32:09,250
+of .. of objective تكبير العدسة الشيئية multiplied
+
+417
+00:32:09,250 --> 00:32:14,230
+مضروب ب that of the eyepiece بتكبير العدسة ال
+
+418
+00:32:14,230 --> 00:32:19,740
+eyepiece وهذا بنسميه ال total magnification طيبطيب
+
+419
+00:32:19,740 --> 00:32:24,840
+ال numerical aperture مصطلح لازم نذكره قبل نشرح
+
+420
+00:32:24,840 --> 00:32:31,920
+مبدأ عمل العدسة ال oil immersion يعني هاي الشغلات
+
+421
+00:32:31,920 --> 00:32:36,350
+لازم نذكرها قبل تمام the numerical apertureال
+
+422
+00:32:36,350 --> 00:32:40,510
+numerical aperture هو عبارة عن the amount أو
+
+423
+00:32:40,510 --> 00:32:45,770
+amount of light entering the objective from the
+
+424
+00:32:45,770 --> 00:32:50,410
+microscopic field كمية الضوء اللي بتصل إلى العدسة
+
+425
+00:32:50,410 --> 00:32:55,790
+الشيئية بنسميها numerical aperture طب ماذا يتحكم
+
+426
+00:32:55,790 --> 00:33:00,290
+بكمية الضوء اللي مار إلى العدسة الشيئية؟ حكينا ال
+
+427
+00:33:00,290 --> 00:33:05,360
+condenserوالارس ده فراجم وجود mirror أو لأ وجود
+
+428
+00:33:05,360 --> 00:33:09,180
+filter أو لأ شدة الضوء أصلا في ال light source قرب
+
+429
+00:33:09,180 --> 00:33:13,060
+الcondenser و بعضه عن ال .. ال .. ال stage كل هذه
+
+430
+00:33:13,060 --> 00:33:17,060
+عوامل تؤثر في كمية الضوء اللي مار إلى العدسة
+
+431
+00:33:17,060 --> 00:33:21,860
+الشيئية تمام؟ طيب لو أنا بدي أجي أحسبه بقانون
+
+432
+00:33:21,860 --> 00:33:27,070
+بينحسب PRفي sign لم يو، إيش sign لم يو هذه؟ تشوفوا
+
+433
+00:33:27,070 --> 00:33:34,170
+معايا R هو ال refractive index of glassاللي هو
+
+434
+00:33:34,170 --> 00:33:37,810
+يعني معامل انكسار الزجاج لإنه العدسة هي زجاج يعني
+
+435
+00:33:37,810 --> 00:33:42,110
+الضوء أصلا بيطلع من لمبة زجاجية و بيفوت على
+
+436
+00:33:42,110 --> 00:33:45,930
+الcondenser و عدسته زجاجية بيخترق ال slide اللي هي
+
+437
+00:33:45,930 --> 00:33:50,850
+عدسة زجاجية او اللي هي شريحة زجاجية sorry و بيروح
+
+438
+00:33:50,850 --> 00:33:53,710
+على ال objective أيضا ال objective اللي الضوء بيمر
+
+439
+00:33:53,710 --> 00:33:56,590
+من خلالها هي عدسة زجاجية إذا لازم أنا أستخدم ال
+
+440
+00:33:56,590 --> 00:34:00,830
+refractive index of glass تمام؟طيب الـmu هي عبارة
+
+441
+00:34:00,830 --> 00:34:04,410
+عن إيش الـmu هذه؟ هي الزاوية اللي يصنعها الشعاع
+
+442
+00:34:04,410 --> 00:34:09,010
+اللي يمر بمركز أو منتصف العدسة مع الشعاع اللي يمر
+
+443
+00:34:09,010 --> 00:34:13,930
+بحافة العدسة أو بطرف العدسة، طبعا؟ الشعاع يمر
+
+444
+00:34:13,930 --> 00:34:17,770
+بمركز، تخيلوا أرسموه بشكل وهمي و الشعاع اللي يمر
+
+445
+00:34:17,770 --> 00:34:22,330
+بحافة العدسة، هذه الزاوية بنسميها الـmu أو هذه
+
+446
+00:34:22,330 --> 00:34:25,450
+الـmu، واحدة منهم، طبعا؟ فهذه
+
+447
+00:34:27,650 --> 00:34:32,750
+الزاوية assign لميو يستخدم في حساب ال numerical
+
+448
+00:34:32,750 --> 00:34:36,290
+aperture طيب بيحكيلي هنا ال numerical aperture
+
+449
+00:34:37,410 --> 00:34:41,510
+depend on the radius of the lens تمام؟ هذا ال
+
+450
+00:34:41,510 --> 00:34:48,370
+numerical aperture يعتمد على القطر العدسة اه والله
+
+451
+00:34:48,370 --> 00:34:55,010
+كل ما زاد قطر العدسة تمام؟ شايفين؟ كل ما زاد قطر
+
+452
+00:34:55,010 --> 00:35:02,520
+العدسة معناها انه ال .. ال ..الزاوية ال new ها دي
+
+453
+00:35:02,520 --> 00:35:06,940
+أكبر تمام و محتاجة ضوء أكتر هي دي شايفين كمية
+
+454
+00:35:06,940 --> 00:35:10,020
+الضوء اللي فاتة أكتر فبالتالي ال numerical
+
+455
+00:35:10,020 --> 00:35:14,280
+aperture يعتبد على قطر العدسة هي هنا مثلا مثلا
+
+456
+00:35:14,280 --> 00:35:19,840
+شايفين numerical aperture 95% كمية الضوء كبيرة
+
+457
+00:35:19,840 --> 00:35:24,700
+هنقل numerical aperture قلت كمية الضوء فقلت و قلت
+
+458
+00:35:24,700 --> 00:35:27,640
+أكتر فأكترنتيجة ان ال numerical aperture قليلة،
+
+459
+00:35:27,640 --> 00:35:30,480
+اذا هو بيعبرلي عن ايش كمية الضوء اللى بده تروح على
+
+460
+00:35:30,480 --> 00:35:36,080
+ال objective lens، على العدسة الشيئية، تمام؟ اذا و
+
+461
+00:35:36,080 --> 00:35:39,240
+بنحسبه بالR، refractive index of glass، حكينا لإن
+
+462
+00:35:39,240 --> 00:35:42,780
+العدسة مصنوعة من ال glass، و ايضا ال mu اللى هى ال
+
+463
+00:35:42,780 --> 00:35:47,220
+angle، الزاوية اللى مصنوعة made by، مصنوعة من Ray،
+
+464
+00:35:47,220 --> 00:35:53,690
+شعاريمر بي الحافة العدسة the edge of lens والتاني
+
+465
+00:35:53,690 --> 00:35:59,110
+يمر بي the center of the lens مركز العدسة تمام
+
+466
+00:35:59,110 --> 00:36:06,230
+resolving power قوة الإضاح تمام؟ وطيب جدير بالذكر
+
+467
+00:36:06,230 --> 00:36:10,970
+او يعني حتى اذكر احنا حكينا معلومة مهمة انه
+
+468
+00:36:10,970 --> 00:36:19,030
+العدسات اللي قوة تكبيرهاكبيرة تمام محتاجة ان ضوء
+
+469
+00:36:19,030 --> 00:36:23,130
+اكثر فمحتاجة ان انا مثلا افتح القرص عشان اتمكن انه
+
+470
+00:36:23,130 --> 00:36:29,250
+ان الضوء يصل ال condenser اكثر اما العدسات اللي
+
+471
+00:36:29,250 --> 00:36:34,930
+قوة تكبيرها قليلة زي ال low power lens تمام هى
+
+472
+00:36:34,930 --> 00:36:40,510
+بتكون مش محتاجة ضوء كتير فبالتالي ممكن انا اسكر
+
+473
+00:36:40,510 --> 00:36:43,910
+القرص عشان كمية الضوء اللي نافظة العدسة تكون قليلة
+
+474
+00:36:47,590 --> 00:36:53,950
+resolving power اللي هي قوة التكبير، تمام؟ هي يعني
+
+475
+00:36:53,950 --> 00:36:56,430
+التوظيف لل magnification، بنعبر عن ال
+
+476
+00:36:56,430 --> 00:37:00,070
+magnification باستخدام ال resolving power، بنعبر
+
+477
+00:37:00,070 --> 00:37:01,770
+عن التكبير بقوة الإضاءة
+
+478
+00:37:04,950 --> 00:37:09,230
+يُعرف هذا ال resolving power بإن هو the ability of
+
+479
+00:37:09,230 --> 00:37:12,750
+microscope قدرة الميكروسكوب at the specific
+
+480
+00:37:12,750 --> 00:37:17,210
+magnification على قوة تكبير معينة to distinguish
+
+481
+00:37:17,210 --> 00:37:22,590
+أنه يميز two separate objects situated close to
+
+482
+00:37:22,590 --> 00:37:26,410
+one another أنه يميز ما بين نقطتين أو two objects
+
+483
+00:37:26,410 --> 00:37:29,850
+أو منصرين موجودين في الشريحة أو في العينة المراد
+
+484
+00:37:29,850 --> 00:37:34,150
+فحصها ويكونوا قراب من بعضهم البعضفكل ما كانت
+
+485
+00:37:34,150 --> 00:37:37,070
+المسافة ما بين هدول ال two objects هدول النقطتين
+
+486
+00:37:37,070 --> 00:37:41,310
+أو العنصرين كل ما كانت المسافة ما بينهم قليلة the
+
+487
+00:37:41,310 --> 00:37:46,790
+smaller the distance تمام؟ the smaller the
+
+488
+00:37:46,790 --> 00:37:50,850
+distance هيكون انه ال .. ال .. ال .. ال .. كل ما
+
+489
+00:37:50,850 --> 00:37:55,530
+كانت المسافة ما بينهم صغيرة وتمكن ال microscope من
+
+490
+00:37:55,530 --> 00:37:59,930
+إيضاحهم وتكبيرهم إذا والله هذا ال microscope عنده
+
+491
+00:37:59,930 --> 00:38:05,310
+greater resolution power تمام؟عنده قوة إيضاح أكبر
+
+492
+00:38:05,310 --> 00:38:12,770
+طالما أن هو بيفصللي يعني تفاصيل وبيوضحلي يعني نقاط
+
+493
+00:38:12,770 --> 00:38:16,750
+أو عناصر أو أشياء قريبة جدا من بعضهم البعض المسافة
+
+494
+00:38:16,750 --> 00:38:20,470
+ما بينهم قليلة جدا كانت المسافة ما بينهم قليلة
+
+495
+00:38:20,470 --> 00:38:25,830
+كتير و هو تمكن من إيضاحهم إذا هو عنده greater
+
+496
+00:38:25,830 --> 00:38:31,070
+resolution power تمام؟طيب ال minimum ال distance
+
+497
+00:38:31,070 --> 00:38:34,850
+المسافة ما بين ال two objects ممكن حسابها بهذا
+
+498
+00:38:34,850 --> 00:38:41,640
+القانون تمام؟ و ال .. ال .. هنا عملية ربطThe
+
+499
+00:38:41,640 --> 00:38:45,300
+larger numerical aperture اش يعني larger numerical
+
+500
+00:38:45,300 --> 00:38:48,200
+aperture؟ يعني كمية الضوء فايتة للعدسة الشيئية
+
+501
+00:38:48,200 --> 00:38:53,320
+كبيرة وحكينا انه العدسة الشيئية اللي قوة تكبيرها
+
+502
+00:38:53,320 --> 00:38:57,060
+كبيرة، اللي magnification تبعها كبير هي اللي بدها
+
+503
+00:38:57,060 --> 00:39:02,670
+larger NA تمام؟ بدها larger numerical apertureطيب
+
+504
+00:39:02,670 --> 00:39:08,710
+فمعناها هيكون هذا ال .. ال .. ال .. ال numerical
+
+505
+00:39:08,710 --> 00:39:11,230
+aperture وهذه العدسة اللي .. اللي قوة تكبيرها
+
+506
+00:39:11,230 --> 00:39:16,530
+كبيرة بيوضحلي smaller distance between objects
+
+507
+00:39:16,530 --> 00:39:21,410
+هيوضحلي مسافات .. يعني يفصللي ال objects أو النقات
+
+508
+00:39:21,410 --> 00:39:27,030
+اللي ما بينهم مسافات قليلة جدا، تمام؟ إذا هو عنده
+
+509
+00:39:27,030 --> 00:39:32,030
+efficientأو greater resolution power تمام؟ بيزيد
+
+510
+00:39:32,030 --> 00:39:35,430
+ال numerical aperture بيزيد كمية الضوء اللي فايتة
+
+511
+00:39:35,430 --> 00:39:39,410
+للعدسة الشيئية إذا العدسة الشيئية نفسها هتكون قوة
+
+512
+00:39:39,410 --> 00:39:43,130
+تكبيرها كبيرة عشان هي .. هي اللي بتحتاج ضوء أكبر
+
+513
+00:39:43,130 --> 00:39:48,510
+إذا قوة توضيح هتكون أكبر و هيوضحلي ال objects اللي
+
+514
+00:39:48,510 --> 00:39:51,450
+بينهم مسافات أصغر تمام؟
+
+515
+00:39:54,070 --> 00:39:57,530
+working principle of an oil immersion مبدأ عمل ال
+
+516
+00:39:57,530 --> 00:40:02,550
+oil immersion كيف احنا العدسة ال ال ال 100x او ال
+
+517
+00:40:02,550 --> 00:40:08,010
+oil immersion lens كيف بتشتغل شوفوا معايا نرجع ل
+
+518
+00:40:08,010 --> 00:40:14,570
+ال microscope و نتخيل مع بعض مصار الدوء مصار الدوء
+
+519
+00:40:16,490 --> 00:40:21,930
+طيب الضوء ينتقل من ال light source ممكن يمر بمرقاء
+
+520
+00:40:21,930 --> 00:40:27,590
+او ب filter و بعدين بالقرص بيتحكم بكمية وجوده او
+
+521
+00:40:27,590 --> 00:40:31,670
+كميته اللي فايتة ل ال condenser و ال condenser
+
+522
+00:40:31,670 --> 00:40:37,010
+بيجمعه و بيخليه يعني يفوت في العدسة الشريقية اللي
+
+523
+00:40:37,010 --> 00:40:42,220
+هي 100X او ال oil immersionو احنا متفقين انه مبين
+
+524
+00:40:42,220 --> 00:40:47,280
+ان ال oil immersion هي اكبر عدسة او اكبر قوة تكبير
+
+525
+00:40:47,280 --> 00:40:50,320
+في ال microscope، في ال light microscope، هذا
+
+526
+00:40:50,320 --> 00:40:54,660
+تمام؟ ال microscope الضوءي اللي احنا بنشرحه، تمام؟
+
+527
+00:40:54,660 --> 00:41:01,050
+فمعناها انها محتاجة اكبر كمية ضوءطيب الضوء يمر
+
+528
+00:41:01,050 --> 00:41:06,750
+بأوساط مختلفة glass اللامبة، هواء ال glass ال ..
+
+529
+00:41:06,750 --> 00:41:07,970
+ال .. ال .. ال .. ال .. ال .. ال .. ال .. ال .. ال
+
+530
+00:41:07,970 --> 00:41:08,250
+.. ال .. ال .. ال .. ال .. ال .. ال .. ال .. ال ..
+
+531
+00:41:08,250 --> 00:41:11,670
+ال .. ال .. ال .. ال .. ال .. ال .. ال .. ال .. ال
+
+532
+00:41:11,670 --> 00:41:12,430
+.. ال .. ال .. ال .. ال .. ال .. ال .. ال .. ال ..
+
+533
+00:41:12,430 --> 00:41:12,450
+ال .. ال .. ال .. ال .. ال .. ال .. ال .. ال .. ال
+
+534
+00:41:12,450 --> 00:41:12,970
+.. ال .. ال .. ال .. ال .. ال .. ال .. ال .. ال ..
+
+535
+00:41:12,970 --> 00:41:13,030
+ال .. ال .. ال .. ال .. ال .. ال .. ال .. ال .. ال
+
+536
+00:41:13,030 --> 00:41:13,750
+.. ال .. ال .. ال .. ال .. ال .. ال .. ال .. ال ..
+
+537
+00:41:13,750 --> 00:41:14,830
+.. ال .. ال .. ال .. ال .. ال .. ال .. ال .. ال ..
+
+538
+00:41:14,830 --> 00:41:14,850
+ال .. ال .. ال .. ال .. ال .. ال .. ال .. ال .. ال
+
+539
+00:41:14,850 --> 00:41:19,150
+.. ال ..
+
+540
+00:41:19,150 --> 00:41:23,430
+ال .. ال .. ال
+
+541
+00:41:29,750 --> 00:41:35,750
+للعدسة مية مش كتيرة كما ينبغي تمام؟ مش كتيرة كما
+
+542
+00:41:35,750 --> 00:41:39,810
+ينبغي ليش؟ لإن جزء من هذا الضوء يتشتت نتيجة
+
+543
+00:41:39,810 --> 00:41:45,850
+انتقاله في أوصات مختلفة وانكساراته تمام؟ طيب، طب
+
+544
+00:41:45,850 --> 00:41:49,370
+إيش هم نعمل علشان إحنا نحافظ على كمية الضوء هذه
+
+545
+00:41:49,370 --> 00:41:52,990
+إنها كلها تفوت للعدسة الشيئية أو مايتم تشتت هذا
+
+546
+00:41:52,990 --> 00:41:58,430
+الضوء؟ شوفوا معايا إيش بنساوي نرجع بس لل slide
+
+547
+00:42:01,070 --> 00:42:07,110
+هي هنا الضوء بيمر من زجاج إلى هواء إلى زجاج مرة ..
+
+548
+00:42:07,110 --> 00:42:11,350
+إلى هواء مرة أخرى إلى زجاج فإيش؟ بيحكينا إن هو
+
+549
+00:42:11,350 --> 00:42:16,390
+بيتشتت تمام؟ طيب لكن لو أنا حطيت على الشريحة تبعتي
+
+550
+00:42:16,390 --> 00:42:23,080
+هذه هي الشريحة لو حطيت عليها قطرة زيتoil غمرتها بـ
+
+551
+00:42:23,080 --> 00:42:27,180
+oil فصارت oil و طبعا شغالتي المسافة اللي ما بين ..
+
+552
+00:42:27,180 --> 00:42:30,220
+المسافة اللي ما بين العدسة ال 100x يا بنات و ال
+
+553
+00:42:30,220 --> 00:42:34,860
+slide بتكون كتير قليلة طبعا فإذا شغالتي المساحة أو
+
+554
+00:42:34,860 --> 00:42:38,640
+المسافة ما بينهم بقطرة oil فساعتها العدسة هذه
+
+555
+00:42:38,640 --> 00:42:42,140
+بتكون عدسة مغمورة بالزيت عشان هيك احنا بنحكي عنها
+
+556
+00:42:42,140 --> 00:42:46,420
+اما 100x او بنحكي عنها ال oil emerging العدسة اللي
+
+557
+00:42:46,420 --> 00:42:52,360
+تغمر بالزيت طبعا طيبإيش اللي هيصير بهذه الوضع؟ لو
+
+558
+00:42:52,360 --> 00:42:59,480
+أنا حطيت oil immersion فمعناها إنه ال .. ال .. ال
+
+559
+00:42:59,480 --> 00:43:03,860
+.. الزيت هذا اللي أنا حطيته إليه نفس معامل انكسار
+
+560
+00:43:03,860 --> 00:43:09,470
+الزجاج فبيصير الضوء بيمر منالـ light source بيروح
+
+561
+00:43:09,470 --> 00:43:13,770
+على الزجاج اللي هو زجاج الـ condenser بعدين زجاج
+
+562
+00:43:13,770 --> 00:43:17,890
+الشريحة بعدين بيمر في المنطقة هذه اللي هي مغمورة
+
+563
+00:43:17,890 --> 00:43:21,410
+بال oil اللي يلو نفس معامل انكسار الزجاج يعني إزاي
+
+564
+00:43:21,410 --> 00:43:26,250
+كأنه الضوء ماشي بزجاج أصلا تمام وينتقل إلى العدسة
+
+565
+00:43:26,250 --> 00:43:30,610
+الشيئية اللي هي أيضا مصنوعة من الزجاج إذا أصبح
+
+566
+00:43:30,610 --> 00:43:34,630
+إحنا هيك وحدنا مصاري الضوء أو لا كأنه وحدنا اللي
+
+567
+00:43:34,630 --> 00:43:38,330
+باستخدامالزيت اللي يقيله نفس معامل انكسار الزجاج
+
+568
+00:43:38,330 --> 00:43:42,230
+فزي كأنه الضوء بيمور بنفس المسار من زجاج
+
+569
+00:43:42,230 --> 00:43:46,210
+الcondenser إلى ال slide إلى ال oil إلى زجاج
+
+570
+00:43:46,210 --> 00:43:52,510
+العدسة الشيء يعنيو هيك بنكون احنا بطل في تشتت
+
+571
+00:43:52,510 --> 00:43:56,890
+للضوء لمينا الضوء جمعناه كله لحتى يفوت في العدسة
+
+572
+00:43:56,890 --> 00:44:01,310
+الشيئية ليش؟ لأنه احنا مستخدم قوة تكبير كبيرة عدسة
+
+573
+00:44:01,310 --> 00:44:06,570
+100X فمحتاجين كمية الضوء أكبر محتاجين ان ال NA ال
+
+574
+00:44:06,570 --> 00:44:10,950
+numerical aperture يكون أعلى تمام؟ و مش محتاجة ولا
+
+575
+00:44:10,950 --> 00:44:15,600
+نت في الضوء يتشتت تمام؟ مابدي أي ضوء يتشتتفهي هي
+
+576
+00:44:15,600 --> 00:44:18,900
+فكرة ال oil immersion، الضوء يتشبط نتيجة مروره
+
+577
+00:44:18,900 --> 00:44:23,640
+بمسارات مختلفة، مع وجود ال oil، الضوء رح ينحجب و
+
+578
+00:44:23,640 --> 00:44:30,220
+رح يظل كأنه زي كأنه ماشي بمسار واحد، فبالتالي كله
+
+579
+00:44:30,220 --> 00:44:33,600
+هيفوت في ال .. ال objective و هيك هيكون في عندي
+
+580
+00:44:33,600 --> 00:44:38,480
+قوة إيضاح أفضل و تكبير أفضلنشوف الكلام اللي مكتوب
+
+581
+00:44:38,480 --> 00:44:42,300
+هنا هو نفسه اللي شرحنا و in a beam of light لما
+
+582
+00:44:42,300 --> 00:44:47,080
+تكون عندى حزمة ضوء passes from air into glass it
+
+583
+00:44:47,080 --> 00:44:50,860
+is bend تمام لما بتنتقل من الهواء اللى موجود تحت
+
+584
+00:44:50,860 --> 00:44:54,220
+الcondenser لل glass اللى فى الcondenser بتنكسر
+
+585
+00:44:54,580 --> 00:44:58,320
+طبعا and when it passed back from glass to air و
+
+586
+00:44:58,320 --> 00:45:01,560
+لما ايضا كمان هذا الضوء ينتقل من ال glass تبع
+
+587
+00:45:01,560 --> 00:45:08,060
+الcondenser و الهواء المسافة اللي ما بين الشريحة و
+
+588
+00:45:08,060 --> 00:45:14,300
+العدسة ايضا كمان بينكسر مرة تانية فبالتالي لما
+
+589
+00:45:14,300 --> 00:45:19,310
+بيرجع لمصاره بيكون تشتت جزء منهلما بيرجع على مسار
+
+590
+00:45:19,310 --> 00:45:23,570
+أو بيمشي في مساره باتجاه العدسة الشيئية بيكون احنا
+
+591
+00:45:23,570 --> 00:45:27,510
+فقدنا جزء من هذا الضوء نتيجة انكساراته ينكسر ويرجع
+
+592
+00:45:27,510 --> 00:45:32,230
+ينعكس، ينكسر ويرجع ينعكس، اذا .. اذا اتشتتتمام؟
+
+593
+00:45:32,230 --> 00:45:37,490
+طيب فهذا هيأثر على ال oil immersion objective ليش؟
+
+594
+00:45:37,490 --> 00:45:40,510
+لإنه هيأثر على ال numerical aperture كمية الضوء
+
+595
+00:45:40,510 --> 00:45:44,110
+اللى فايت على ال objective وإحنا متفقين وكررنا
+
+596
+00:45:44,110 --> 00:45:48,270
+مرارا وتكرارا إنه والله كل ما استخدمنا قوة تكبير
+
+597
+00:45:48,270 --> 00:45:52,510
+كبيرة معناها بدي أنقى كبير بدي كمية ضوء أعلى تصل
+
+598
+00:45:52,510 --> 00:45:57,390
+أو أكبر تصل ل ال objective lensتمام؟ فإذا أيضا
+
+599
+00:45:57,390 --> 00:46:00,770
+هيأثر على ال resolving power على قوة الإيضاح لهذه
+
+600
+00:46:00,770 --> 00:46:06,030
+العدسة فبالتالي المفروض أنه أنا أمنع انكسار الضوء
+
+601
+00:46:06,030 --> 00:46:14,420
+هذا من خلال أن أنا أحل الهواء اللي موجودالهواء
+
+602
+00:46:14,420 --> 00:46:21,280
+اللي موجود بين العينة و العدسة أشغله ب .. بقطرة
+
+603
+00:46:21,280 --> 00:46:27,140
+زيت، تمام؟ هذا الزيت .. هذا الزيت إله the same
+
+604
+00:46:27,140 --> 00:46:30,600
+optical properties of a glass، إله نفس الخصائص
+
+605
+00:46:30,600 --> 00:46:36,280
+الضوئية أو الخصائص للزجاج، يعني إله نفس معامل
+
+606
+00:46:36,280 --> 00:46:42,980
+انكسارالضوء في الزجاج فإذا لما الضوء بيطلع من
+
+607
+00:46:42,980 --> 00:46:48,000
+الcondenser بيمور بال slide و بعدين بيمور بالزيت
+
+608
+00:46:48,000 --> 00:46:50,960
+نفس معامل انكسار الزجاج ده يعني ذلك إنه ينتقل من
+
+609
+00:46:50,960 --> 00:46:54,820
+زجاج إلى زجاج إلى ما يشبه الزجاج اللي هو ال oil و
+
+610
+00:46:54,820 --> 00:47:01,530
+من ثم إلىال oil emerging العدسة اللي هي أيضا زجاج
+
+611
+00:47:01,530 --> 00:47:06,670
+اذا انا هيك وحدت مصار الضوء منعت تشتته لميته كله
+
+612
+00:47:06,670 --> 00:47:11,770
+وجمعته عشان يخترق العدسة الشيئية لإنها محتاجة NA
+
+613
+00:47:11,770 --> 00:47:15,870
+أكبر لإن قوة تكبيرها عالية حكينا هي أعلى شمية X في
+
+614
+00:47:15,870 --> 00:47:20,290
+ال microscope في ال light microscopeتمام؟ اذا انا
+
+615
+00:47:20,290 --> 00:47:25,530
+هيك اش بعمل collecting ل ال light تمام؟ بجمع الضوء
+
+616
+00:47:25,530 --> 00:47:30,650
+و بخليه يختارق العينة اذا ال oil فائدة ال oil
+
+617
+00:47:30,650 --> 00:47:34,590
+بيعطيني better resolution تمام؟ و brighter image
+
+618
+00:47:34,590 --> 00:47:41,430
+وضوء يعني صورة مضيئة اكثر و واضحة اكثروحكينا ليش
+
+619
+00:47:41,430 --> 00:47:45,670
+بسمي العدسة oil immersion لإن المسافة ما بين ال
+
+620
+00:47:45,670 --> 00:47:49,410
+slide و العدسة بتكون قليلة فلما أحط قطرة الزيت
+
+621
+00:47:49,410 --> 00:47:53,670
+العدسة تنغمر في قطرة الزيت هذه فبتصبح المسافة
+
+622
+00:47:53,670 --> 00:47:58,850
+مشغولة من هنا بالزيت فلإنها بتنغمر بالزيت بنسميها
+
+623
+00:47:58,850 --> 00:48:02,450
+oil immersion طيب
+
+624
+00:48:04,410 --> 00:48:10,830
+types of microscopy تمام؟ أنواع الميكروسكوبات في
+
+625
+00:48:10,830 --> 00:48:13,710
+عندي أنواع للميكروسكوبات مش كلها واحد احنا اللي
+
+626
+00:48:13,710 --> 00:48:16,730
+شرحناه هو ال light microscope هو الميكروسكوب
+
+627
+00:48:16,730 --> 00:48:21,730
+الضوءي لكن في عندي أنواع أخرى من الميكروسكوب زي ال
+
+628
+00:48:21,730 --> 00:48:26,010
+ultraviolet microscopeالـ Ultraviolet Microscopy
+
+629
+00:48:26,010 --> 00:48:29,810
+أو Ultraviolet Microscope هو بيستخدم ال light
+
+630
+00:48:29,810 --> 00:48:37,750
+source عنده UV Ultraviolet ضوء فوق بنفسجي أشعة فوق
+
+631
+00:48:37,750 --> 00:48:46,230
+بنفسجية و طبعا قوة التوضيح بيوضح المسافة
+
+632
+00:48:46,230 --> 00:48:49,490
+ما بين ال two objects ممكن تكون point one
+
+633
+00:48:49,490 --> 00:48:54,590
+micrometerيعني قوة توضيح عالية، تمام؟ احنا إيش
+
+634
+00:48:54,590 --> 00:48:57,730
+عرفنا ال resolution؟ حكينا هي قوة التوضيح ما بين
+
+635
+00:48:57,730 --> 00:49:04,350
+two objects أو نقطتين، يعني two points في الصورة
+
+636
+00:49:04,350 --> 00:49:07,670
+أو في العيلة المراد فحصها، ال distance ما بينهم
+
+637
+00:49:07,670 --> 00:49:12,530
+كتير قليلة، تمام؟ هو بوضح لحد distance point one
+
+638
+00:49:12,530 --> 00:49:20,010
+micrometer، طيببغض النظر عن ان ال UV light is
+
+639
+00:49:20,010 --> 00:49:23,350
+invisible احنا انا مابشوف الأشعة فوق البنفسجية
+
+640
+00:49:23,350 --> 00:49:26,330
+يعني صعب ان اشوف ال .. يعني أنظر على العدسات
+
+641
+00:49:26,330 --> 00:49:30,810
+العينية واشوف صورة، تمام؟ فالصورة هاي اللي
+
+642
+00:49:30,810 --> 00:49:34,330
+بيلتقطها المايكروسكوب باستخدام ال ultraviolet
+
+643
+00:49:34,330 --> 00:49:40,920
+مايكروسكوبيهو راح ينقلها إلى photographic plate أو
+
+644
+00:49:40,920 --> 00:49:46,800
+fluorescent screen إلى أشعة أو صورة يعني شاشة
+
+645
+00:49:46,800 --> 00:49:52,540
+فلورنسية فتبدو الصورة مضيقة على هذه الشاشة وممكن
+
+646
+00:49:52,540 --> 00:49:57,290
+رؤيتهاطمام؟ إذا أنا ما بتمكن من رؤية الصورة على
+
+647
+00:49:57,290 --> 00:50:01,910
+العدسات العينية للمايكروسكوب لإنه أنا أصلا مابشوفش
+
+648
+00:50:01,910 --> 00:50:06,370
+العدسات .. الأشعة فوق البنفسجية مابشوفش مصدر ضوء
+
+649
+00:50:06,370 --> 00:50:10,170
+بإنتي تعالي تطلعي في .. في .. في مكان معتم إنتي مش
+
+650
+00:50:10,170 --> 00:50:14,030
+شايفة فيه ضوء فمش هتشوفي صورة لازم تنقل الصورة إلى
+
+651
+00:50:14,030 --> 00:50:19,450
+microscope إلى .. إلى photographic plate إلى ..
+
+652
+00:50:19,450 --> 00:50:24,580
+يعني ..جهاز مخصص لنقل الصور أو شاشة فلورنسية أو
+
+653
+00:50:24,580 --> 00:50:29,300
+جهاز computer لحتى أشوفها و العدسات بتكون مصنوعة
+
+654
+00:50:29,300 --> 00:50:35,120
+من liquids كمانلأ علشان هي أفضل إشي حكينا لل UV
+
+655
+00:50:35,120 --> 00:50:39,420
+light تمام؟ هي أفضل إشي لـ UV light طيب
+
+656
+00:50:39,420 --> 00:50:43,380
+fluorescence ميكروسكوبي نوع من أنواع الميكروسكوبات
+
+657
+00:50:43,380 --> 00:50:49,080
+اللي أيضا بيستخدم ال UV light ولكن هنا العينة
+
+658
+00:50:49,080 --> 00:50:53,040
+تبعتي بتكون مصبوغة بصبغة بنسميها fluorescence dye
+
+659
+00:50:53,040 --> 00:50:57,160
+هذه ال fluorescence dye هي عبارة عن صبغات أول ما
+
+660
+00:50:57,160 --> 00:51:02,020
+بي .. يعني صبغةأول ما بيضروا فيها ال .. ال .. أشعة
+
+661
+00:51:02,020 --> 00:51:06,320
+ال UV light بتتأين و بيصير ت .. بيصير لامعة، بيصير
+
+662
+00:51:06,320 --> 00:51:10,780
+تعطي إشعاع فلورنزي، تمام؟ فبشوف الصورة مشعة بهذا
+
+663
+00:51:10,780 --> 00:51:14,620
+الشكل تحت ال microscope، تمام؟ يعني نفس فكرة ال
+
+664
+00:51:14,620 --> 00:51:18,000
+ultraviolet، بس اللهم بدل ما أنقل الصورة على
+
+665
+00:51:18,000 --> 00:51:21,460
+fluorescent screen أو photographic plate أو
+
+666
+00:51:21,460 --> 00:51:27,680
+computer systemالعدسة نفسها .. العينة نفسها بتكون
+
+667
+00:51:27,680 --> 00:51:31,720
+مصبوغة ب fluorescent dye و بشوفها لامعة تحت ال
+
+668
+00:51:31,720 --> 00:51:35,160
+microscope تمام؟ هذا فكرة ال fluorescent
+
+669
+00:51:35,160 --> 00:51:39,790
+microscope طيب ال dark field microscopeال dark
+
+670
+00:51:39,790 --> 00:51:43,470
+field microscope هو عبارة عن microscope بيعطيني
+
+671
+00:51:43,470 --> 00:51:49,330
+black background اللي هو خلفية معتمة للصورة أو
+
+672
+00:51:49,330 --> 00:51:53,930
+للعينة و فقط البكتيريا أو الخلية أو العنصر المراد
+
+673
+00:51:53,930 --> 00:51:58,760
+رؤيته هو اللي بيكون مضيقتمام ال suspended bacteria
+
+674
+00:51:58,760 --> 00:52:01,740
+أو ال element العنصر اللي مراد رؤيته هو اللي بيكون
+
+675
+00:52:01,740 --> 00:52:08,100
+bright لامع و مضيق تمام كيف بيتم تصوير بهذا ال ..
+
+676
+00:52:08,100 --> 00:52:12,480
+بهذه القالية ال .. ال microscope هذا عنده special
+
+677
+00:52:12,480 --> 00:52:17,260
+condenser هذا الcondenser بيجمع الضوء تمام و فقط
+
+678
+00:52:17,260 --> 00:52:20,840
+بيخليه يخترق مين؟ بيخليه يخترق ال object اللي على
+
+679
+00:52:20,840 --> 00:52:25,360
+ال .. على العدسة على ال slide تمام بيخترق ال .. ال
+
+680
+00:52:25,360 --> 00:52:29,250
+.. ال .. ال ..فقط اللي هو ال bacteria أو ال
+
+681
+00:52:29,250 --> 00:52:33,810
+element المراد رؤيته على ال slide وينتقل الضوء منه
+
+682
+00:52:33,810 --> 00:52:37,570
+إلى العدسة الشيئية، أي شيء آخر، في حوالين هذا ال
+
+683
+00:52:37,570 --> 00:52:42,110
+.. ال element يتبدد فاحنا بنشوف كل إشي معتم إلا
+
+684
+00:52:42,110 --> 00:52:47,360
+الخلية أو العنصر المراد رؤيته بيكون مضيع، تمام؟ف
+
+685
+00:52:47,360 --> 00:52:50,560
+.. وعندي نوعة رابع اللي هو ال face contrast
+
+686
+00:52:50,560 --> 00:52:54,600
+مايكروسكوبي ال face contrast مايكروسكوبي هو طبعا
+
+687
+00:52:54,600 --> 00:52:58,460
+احنا في الأنواع السابق ذكرها احنا بنشوف الخلايا و
+
+688
+00:52:58,460 --> 00:53:03,700
+البكتيريا و الفطريات و الطفاليات اي خلايا سواء
+
+689
+00:53:03,700 --> 00:53:08,160
+كانت طبعا يعني animal cells or plant cells او حتى
+
+690
+00:53:08,160 --> 00:53:16,320
+كمان اللي بروكاريوت تمام؟بنشوفها و هي ميتة الخلايا
+
+691
+00:53:16,320 --> 00:53:20,960
+يعني لابد من قتلها و نجففها و نصبغها should be
+
+692
+00:53:20,960 --> 00:53:25,760
+killed dried and stained و نصبغها عشان نتمكن من
+
+693
+00:53:25,760 --> 00:53:28,540
+رؤيتها لكن هذا ال microscope اللي هو ال face
+
+694
+00:53:28,540 --> 00:53:33,520
+contrast مايكروسكوبي بيورجيني
+
+695
+00:53:33,520 --> 00:53:37,970
+الخلايا visualize the living bacteria andcells
+
+696
+00:53:37,970 --> 00:53:42,070
+تمام بيورجيني الخلايا و هي حية كيف والله انه انا
+
+697
+00:53:42,070 --> 00:53:46,450
+في عندى معامل معامل انكسار مختلف لمرور الضوء اثناء
+
+698
+00:53:46,450 --> 00:53:55,390
+خلال كائن حي وأثناء مرور الضوء في كائن أو خلية
+
+699
+00:53:55,390 --> 00:54:00,130
+ميتة، تمام؟ فهذا ال .. ال .. ال microscope يوظف
+
+700
+00:54:00,130 --> 00:54:04,650
+هذا الفرق في المعامل الانكسار للضوء، تمام؟ إذا
+
+701
+00:54:04,650 --> 00:54:07,890
+المعامل الانكسار للضوء يختلف أثناء مروره في خلية
+
+702
+00:54:07,890 --> 00:54:12,580
+حيةعن مروره في خلية ميتة هذا ال microscope enhance
+
+703
+00:54:12,580 --> 00:54:16,820
+بيعزز أو بيستخدم ال slight difference هذا الفرق ما
+
+704
+00:54:16,820 --> 00:54:19,760
+بين ال .. ال .. ال .. هذا الفرق في ال refractive
+
+705
+00:54:19,760 --> 00:54:24,120
+index في معامل الانكسار ما بين مروره في خلايا حية
+
+706
+00:54:24,120 --> 00:54:27,840
+و ما بين مروره في الوسط أو الخلايا الميتة المحيطة
+
+707
+00:54:27,840 --> 00:54:34,560
+فيها فبالتالي بيوضحلي ال .. الخلايا الحيةالكترون
+
+708
+00:54:34,560 --> 00:54:38,580
+مايكروسكوبي طبعا هو تقريبا افضل مايكروسكوب و اعلى
+
+709
+00:54:38,580 --> 00:54:43,540
+قوة تكبير لمايكروسكوب بتقريبا بيعطيني قوة تكبير
+
+710
+00:54:43,540 --> 00:54:50,120
+1500x او 2000x وفي قوة عند خمسين الف اكس تمام
+
+711
+00:54:50,120 --> 00:54:51,300
+بيكون كبير
+
+712
+00:54:54,250 --> 00:54:59,850
+الإلكترون مايكروسكوبي فيه منه نوعين طبعا فيه ال
+
+713
+00:54:59,850 --> 00:55:02,850
+transmission electron microscope بيعطيني صورة
+
+714
+00:55:02,850 --> 00:55:06,490
+ثنائية الأبعادو في scanning electron microscope
+
+715
+00:55:06,490 --> 00:55:14,650
+اللي هو ثلاثي الأبعاد، تمام؟ فهو عنده يعني .. ب ..
+
+716
+00:55:14,650 --> 00:55:16,990
+ب .. احنا .. احنا بنفضل نستخدمه عن ال light
+
+717
+00:55:16,990 --> 00:55:20,210
+microscope ليش؟ لإن ال light microscope يعني مقنعة
+
+718
+00:55:20,210 --> 00:55:24,550
+فيه عنده decreased efficiency و resolving power،
+
+719
+00:55:24,550 --> 00:55:28,390
+تمام؟ لكن هو .. هذا عنده high degree of resolving
+
+720
+00:55:28,390 --> 00:55:33,900
+power، ال electron microscopyطيب routine use of
+
+721
+00:55:33,900 --> 00:55:37,400
+microscope هاي ال .. النقاط أنا راح أذكرها بشكل
+
+722
+00:55:37,400 --> 00:55:42,300
+سرد سريع ولكن راح أشرحها بالتفصيل داخل المختبر على
+
+723
+00:55:42,300 --> 00:55:46,500
+المختبر .. على ال .. ال microscope ده نفسه تمام؟ ف
+
+724
+00:55:46,500 --> 00:55:50,260
+.. لحتى يكون أسهل و أوضحأول إشي أنا لما بدي أجي
+
+725
+00:55:50,260 --> 00:55:53,280
+أستخدم ال microscope لازم أتأكد إن هو موجود على
+
+726
+00:55:53,280 --> 00:55:56,560
+farm bench على bench ثابت مافيش .. ال bench هذا مش
+
+727
+00:55:56,560 --> 00:56:00,660
+موجود جنب جهاز طلاجة أو centrifuge أو إشي بيعملي
+
+728
+00:56:00,660 --> 00:56:05,300
+اهتزازات لإن هذا هيأثر على وضوح الصورة تمام؟و make
+
+729
+00:56:05,300 --> 00:56:08,140
+sure that is not exposed to direct sunlight انه
+
+730
+00:56:08,140 --> 00:56:13,200
+هذا الجهاز مش لازم يتعرض لأشعة الشمس مباشرة لإنه
+
+731
+00:56:13,200 --> 00:56:20,380
+ممكن يطلف، يصدق، يخرب و أيضا ال user المستخدم
+
+732
+00:56:20,380 --> 00:56:24,680
+لmicroscope لازم يجلس على ارتفاع مناسب من ال
+
+733
+00:56:24,680 --> 00:56:28,260
+microscope should be seated at the correct height
+
+734
+00:56:28,260 --> 00:56:35,650
+يعني على ارتفاع مناسب من ال microscopeselect the
+
+735
+00:56:35,650 --> 00:56:40,310
+appropriate source of light لازم نختار مصدر الضوء
+
+736
+00:56:40,310 --> 00:56:45,950
+سواء كان لمبة أو daylight و بشغل الجهاز بالكهربا
+
+737
+00:56:45,950 --> 00:56:48,870
+بضوي الضوء من عند الكابسا اللي حكيتلكم عنها بتحكم
+
+738
+00:56:48,870 --> 00:56:54,260
+بشدة الإضاءة من أيضا المكان الليأشرتلكم إليه في
+
+739
+00:56:54,260 --> 00:57:00,260
+أول ال slides و طبعا بحط العينة تبعتي على ال stage
+
+740
+00:57:00,260 --> 00:57:05,620
+بثبتها بال clips و طبعا بتأكد إن ال slide من تحت
+
+741
+00:57:05,620 --> 00:57:10,500
+طبعا تكون ناشفة لإن أي بقايا صبغ عليها أوسائل راح
+
+742
+00:57:10,500 --> 00:57:15,460
+يأثر على وضوح الصورة فبمسحها قبل ما أحطها على ال
+
+743
+00:57:15,460 --> 00:57:20,040
+slide على ال stage select the objective to be used
+
+744
+00:57:22,490 --> 00:57:28,510
+بختار العدسة الشيئية المراد استخدامها وهنا يفضل يا
+
+745
+00:57:28,510 --> 00:57:32,410
+بنات إذا أنا بدي أستخدم قوة تكبير كبيرة يفضل إن
+
+746
+00:57:32,410 --> 00:57:36,010
+أنا أبدأ بقوة تكبير صغيرة بعدين أنتقل لكبيرة it's
+
+747
+00:57:36,010 --> 00:57:38,490
+better to begin examination with low power with
+
+748
+00:57:38,490 --> 00:57:43,850
+A10X مثلاتمام؟ و طبعا ليش .. ليش أنا ب .. ببدأ في
+
+749
+00:57:43,850 --> 00:57:46,510
+العشرة X؟ عشان أ .. أظبط ال .. ال .. ال
+
+750
+00:57:46,510 --> 00:57:51,930
+illumination، الإضاءة، أجيب ال field، أجيب الصورة،
+
+751
+00:57:51,930 --> 00:57:57,450
+تمام؟ أتأكد إن كل ال .. الأمور تمام، بعدين أنتقل
+
+752
+00:57:57,450 --> 00:58:00,410
+لل high power field، اللي هي أربعين X أو حتى oil
+
+753
+00:58:00,410 --> 00:58:05,520
+immersion، ممكن أنتقل لإلهاطبعا كيف أنا بجيب ال
+
+754
+00:58:05,520 --> 00:58:09,940
+field? bring the objective as close as possible to
+
+755
+00:58:09,940 --> 00:58:15,780
+the slide preparation يعني أنا بقرب المسافة ما بين
+
+756
+00:58:15,780 --> 00:58:20,160
+ال stage اللي عليها ال slide و ما بين ال objective
+
+757
+00:58:20,160 --> 00:58:25,100
+lenses كيف؟ بحرك ال course adjustmentمجرد ما اجيب
+
+758
+00:58:25,100 --> 00:58:29,720
+course adjustment ممكن انا اتحكم بكمية الضوء اللي
+
+759
+00:58:29,720 --> 00:58:34,540
+مارة من خلال ان انا اعمل adjustment لل light و ..
+
+760
+00:58:34,540 --> 00:58:40,630
+و .. لل condenserممكن أفتح القرص أو أسكره حسب كمية
+
+761
+00:58:40,630 --> 00:58:43,130
+الضوء اللي بضيقها و حسب نوع العدسة اللي أنا
+
+762
+00:58:43,130 --> 00:58:47,330
+بستخدمها و طبعا حكينا على ال low power القرص يفضل
+
+763
+00:58:47,330 --> 00:58:50,990
+أنه يكون مغلق و بفتحه على ال high power و ال oil
+
+764
+00:58:50,990 --> 00:58:55,490
+immersion طيب و طبعا هنا هاي بيحكيلي the wider the
+
+765
+00:58:55,490 --> 00:59:00,670
+condenser aperture كلما زاؤ وسعت فتحة ال aperture
+
+766
+00:59:00,670 --> 00:59:04,990
+فتحة الحجاباللي .. اللي تحت ال condenser طبعا
+
+767
+00:59:04,990 --> 00:59:09,110
+معناها كمية ضوء أكبر هتفوت ل ال condenser معناها
+
+768
+00:59:09,110 --> 00:59:13,650
+الصورة إيش هتكون brighter مضيقة أكتر ولامعة أكتر
+
+769
+00:59:13,650 --> 00:59:18,610
+طبعا و أحيانا .. أحيانا بتكون ال details قليلة
+
+770
+00:59:18,610 --> 00:59:23,250
+اللي بنشوفها في هاي الشغلة إلا إذا كنت بستخدميعني
+
+771
+00:59:23,250 --> 00:59:27,890
+عدسة كبيرة فلأ يعني ال wider condenser هيعطيني
+
+772
+00:59:27,890 --> 00:59:33,830
+brighter و برضه كمان أوضح فعشان هيك لازم أن أنا
+
+773
+00:59:33,830 --> 00:59:37,830
+أسكره في العدسات اللي قوة تكبيرها قليلة لإن ممكن
+
+774
+00:59:37,830 --> 00:59:40,590
+تكون ال details مش واضحة كتير يعني أنا بس شايفها
+
+775
+00:59:40,590 --> 00:59:44,650
+صورة لامعة فقط لا غير تمام؟ إذا في هذه دولة
+
+776
+00:59:44,650 --> 00:59:48,350
+النقطتين ينطبقوا على العدسة 10xإنه كل ما كان
+
+777
+00:59:48,350 --> 00:59:50,830
+الـcondenser فاتح أكتر، يعني ضوء أكتر، يعني
+
+778
+00:59:50,830 --> 00:59:53,870
+الـdetails مابشوفهاش كتير، بتكون الصورة بس لامعة
+
+779
+00:59:53,870 --> 00:59:57,750
+على الفاضي، تمام؟ فإذا كان الـsmaller aperture،
+
+780
+00:59:57,750 --> 01:00:02,150
+يعني great will be contrast، يعني في تباين في
+
+781
+01:00:02,150 --> 01:00:06,530
+الصورة، فبتكون واضحة أكتر، هذا ينطبق على 10X، لكن
+
+782
+01:00:06,530 --> 01:00:11,150
+على العدسات اللي قوة تكبيرها أعلى، العكس، يعني أنا
+
+783
+01:00:11,150 --> 01:00:13,850
+محتاجة wider condenser ومحتاجة ضوء أكتر
+
+784
+01:00:16,710 --> 01:00:20,330
+تمام؟ العادة الصورة كيف أنا بشوفها؟ بشوفها upside
+
+785
+01:00:20,330 --> 01:00:26,450
+down مقلوبة و معكوسة كمان upside down and in
+
+786
+01:00:26,450 --> 01:00:29,110
+opposite direction يعني اللي تحت فوق و اللي فوق
+
+787
+01:00:29,110 --> 01:00:33,710
+تحت و على المعكوسة
+
+788
+01:00:33,710 --> 01:00:40,510
+يعني اليمين يسار و اليسار يمين عشان هيك في مكان
+
+789
+01:00:40,510 --> 01:00:43,510
+لتحريك الشريحة
+
+790
+01:00:44,850 --> 01:00:52,430
+على ال stage هنا هنا
+
+791
+01:00:52,430 --> 01:00:58,290
+هادول .. هادول الإتنينبيحركولي الشريحة، طبعا؟
+
+792
+01:00:58,290 --> 01:01:04,130
+فواحد بيحركها لفوق وتحت وواحد بيحركها يمين يسار،
+
+793
+01:01:04,130 --> 01:01:09,410
+طبعا؟ فلما أنا بكون بروح يمين على .. لما بحرك
+
+794
+01:01:09,410 --> 01:01:13,210
+الشريحة
+
+795
+01:01:13,210 --> 01:01:17,350
+إلى اليمين على ال stage معناه أنا بكون بروح لليسار
+
+796
+01:01:17,350 --> 01:01:23,290
+في الصورةو لما بحرك ال .. ال .. هدول يعني بحرك ال
+
+797
+01:01:23,290 --> 01:01:26,890
+.. ال slide إلى اليسار على ال stage معناها أنا
+
+798
+01:01:26,890 --> 01:01:30,010
+بروح لليمين في الصورة لإن الصورة معكوسة و نفس
+
+799
+01:01:30,010 --> 01:01:33,490
+الاشي لو أنا كنت بطلع لفوق على ال .. في ال .. في
+
+800
+01:01:33,490 --> 01:01:37,450
+ال slide تمام معناها أنا بكون بنزل لتحت في الصورة
+
+801
+01:01:37,450 --> 01:01:41,420
+و لما بنزل لتحت في ال slide بكون بطلعلفوق في
+
+802
+01:01:41,420 --> 01:01:44,700
+الصورة لإن الصورة بتكون upside-down إذا الإتجار
+
+803
+01:01:44,700 --> 01:01:47,380
+بتكون معكوسة على ال microscope وهذه شغل .. شغل
+
+804
+01:01:47,380 --> 01:01:51,820
+لازم ينتبهلها كتير منيح و هنتعلمها مع الممارسة
+
+805
+01:02:00,350 --> 01:02:05,210
+تمام بعد هيك ممكن أصل ل .. يعني أغير على 40x بعد
+
+806
+01:02:05,210 --> 01:02:10,090
+ما جبت الصورة باستخدام ال 10x و أبدأ أفحص ال .. ال
+
+807
+01:02:10,090 --> 01:02:15,370
+.. العينة تبعتي و ممكن انا إذا محتاجة oil
+
+808
+01:02:15,370 --> 01:02:22,170
+immersion أو 100x lenses فبحط قطرة زيت على الشريحة
+
+809
+01:02:22,170 --> 01:02:28,870
+تبعتي علشان أتمكن من شو ..رؤية الأجزاء والصورة
+
+810
+01:02:28,870 --> 01:02:32,670
+موضحة أكتر هي عندي طبعا ال oil immersion هنستخدمها
+
+811
+01:02:32,670 --> 01:02:36,190
+لما تعملوا ال blood smear مسحة الدم وهتستخدموها في
+
+812
+01:02:36,190 --> 01:02:40,630
+مختبرات ال microbiology لما تستخدموا ال asbestos
+
+813
+01:02:40,630 --> 01:02:46,010
+تاني اللي هي صبغة لسباغة البكتيريا وكل ما بتكون
+
+814
+01:02:46,010 --> 01:02:49,730
+تصبغوا بكتيريا أو خلايا راح تستخدموا ال oil
+
+815
+01:02:49,730 --> 01:02:54,880
+immersionطيب care, cleaning and repair of the
+
+816
+01:02:54,880 --> 01:02:57,640
+microscope كيف أنا أقعدتني في ال microscope؟ أول
+
+817
+01:02:57,640 --> 01:03:03,140
+إشي ال microscope طبعا يعني معرب للتلف فلازم أنا
+
+818
+01:03:03,140 --> 01:03:07,020
+أراعي أو يكون في عندي اعتبارات تراعى أثناء
+
+819
+01:03:07,020 --> 01:03:10,910
+استخدامهأول إشي بدي أمسكه بإيديا تنتين ممنوع أمسكه
+
+820
+01:03:10,910 --> 01:03:14,690
+بإيد واحدة لإنه ممكن يعني إذا واجع خلاص خيرب يتكسر
+
+821
+01:03:14,690 --> 01:03:18,310
+و not in use إذا أنا ما استخدمته فمعناها إن أنا
+
+822
+01:03:18,310 --> 01:03:22,750
+لازم أحمي أول إشي من الغبرة من الرطوبة من ال
+
+823
+01:03:22,750 --> 01:03:29,370
+sunlight فيعنيإله بيكون sack إله حقيبة بلاستيكية
+
+824
+01:03:29,370 --> 01:03:33,390
+أو بكات أو cover بيغطيه فلازم أكون مغطية أكون
+
+825
+01:03:33,390 --> 01:03:37,630
+فاصلة من الكهربا مش لازم يضل مشبوك بالكهربا و لازم
+
+826
+01:03:37,630 --> 01:03:44,420
+يكون طبعا ..إذا كان أنا .. إذا بدي أخليه في مكان
+
+827
+01:03:44,420 --> 01:03:48,680
+الشغل جاهز يعني لأي وقت بستخدمه فيه بس على الأقل
+
+828
+01:03:48,680 --> 01:03:53,020
+يعني أغطيه بال cover تبعه تمام؟ في ال humidity
+
+829
+01:03:53,020 --> 01:03:56,960
+climate في المناطق اللي الجو تبعها رطب ممكن إنه
+
+830
+01:03:56,960 --> 01:03:59,880
+يصدق هذا ال microscope نتيجة تكوين قطرات ندى عليه
+
+831
+01:03:59,880 --> 01:04:04,420
+وهذا فبيصدق فيفضل إنه ال cover تبعه اللي هو ال
+
+832
+01:04:04,420 --> 01:04:08,160
+plastic bag تبعته اللي هو الكيس أو الحقيبة اللي
+
+833
+01:04:08,160 --> 01:04:14,850
+بتغطيهفإنه تكون عليها silica gel مادة silica gel
+
+834
+01:04:14,850 --> 01:04:18,970
+وهي مادة drying agent عامل مجفف يمتص الرطوبة عشان
+
+835
+01:04:18,970 --> 01:04:25,030
+العدسات ما تخرب وما ينمو عليها فطر والهيكل المعدني
+
+836
+01:04:25,030 --> 01:04:26,470
+للميكروسكوب لا يصدق
+
+837
+01:04:29,100 --> 01:04:33,220
+طبعا اكيد at the end of each day في نهاية كل يوم
+
+838
+01:04:33,220 --> 01:04:37,840
+لازم انا انظف العدسات هاي تمام؟ انظف ال stage من
+
+839
+01:04:37,840 --> 01:04:42,040
+بقايا الزيت و الشرايح انظف ال objective lenses و
+
+840
+01:04:42,040 --> 01:04:45,000
+ال eye lenses و الcondenser طبعا كيف انا .. طبعا
+
+841
+01:04:45,000 --> 01:04:47,720
+الcondenser يا بنات ممكن تتجمع عليه غبار عدسته
+
+842
+01:04:47,720 --> 01:04:52,980
+فمهم جدا انك انتي تمسحيه قبل استخدامهو طبعا كيف
+
+843
+01:04:52,980 --> 01:04:57,260
+بنظفهم؟ بنظفهم بمادة مخصصة لها اللي هي للزايلين و
+
+844
+01:04:57,260 --> 01:05:01,080
+يفضل إن أنا ما أستخدم الكحول إذا .. إلا إذا مش
+
+845
+01:05:01,080 --> 01:05:04,380
+متوفر زايلين يعني فيفضل إن أنا أبعد عن الكحول لإن
+
+846
+01:05:04,380 --> 01:05:08,560
+الكحول بسرع تلف العدساتrepair of the microscope
+
+847
+01:05:08,560 --> 01:05:13,680
+بيحكيلي انه لو تعرض ال .. ال .. ال .. ال
+
+848
+01:05:13,680 --> 01:05:18,240
+microscope لتلف تمام صارله damaged اما optically
+
+849
+01:05:18,240 --> 01:05:21,180
+يعني نظام العدسات فيه خربط او mechanically انكسر
+
+850
+01:05:22,490 --> 01:05:26,650
+خرب ف it's better to send it or .. or the damaged
+
+851
+01:05:26,650 --> 01:05:30,310
+part to reliable scientific instrument repairer or
+
+852
+01:05:30,310 --> 01:05:33,890
+the .. to the manufacturer يعني إما ب .. ب .. يعني
+
+853
+01:05:33,890 --> 01:05:39,130
+بارسله لمهندس صيانة للأجهزة الطبية أو للجهة
+
+854
+01:05:39,130 --> 01:05:43,330
+المصنعة له لحتى هي لإن هي بتكون أكتر حد فاهم تصنيع
+
+855
+01:05:43,330 --> 01:05:49,770
+هذا الجهاز و .. فبتكون أفضل حد ممكن يصلحه تمام؟ و
+
+856
+01:05:49,770 --> 01:05:56,940
+..يعني إذا كان التلف إشي بسيط ممكن أنتي ت .. يعني
+
+857
+01:05:56,940 --> 01:06:00,200
+ال .. يعني كان simple measures يعني أشياء بسيطة
+
+858
+01:06:00,200 --> 01:06:05,240
+جدا أنتي ممكن تعمليها فعادي يعني ممكن أنتي تقومي
+
+859
+01:06:05,240 --> 01:06:09,730
+بالعملية هاييعني damage كبير optically or
+
+860
+01:06:09,730 --> 01:06:12,870
+mechanically لأ ودي إما المهندس صيانة أو الشركة
+
+861
+01:06:12,870 --> 01:06:17,370
+المصنعة هيك بنكون أوصلنا لنهاية محاضرتنا لليوم
+
+862
+01:06:17,370 --> 01:06:20,010
+يعطيكم العافية اتمنى تكونوا استفدتوا ان شاء الله
+
+863
+01:06:20,010 --> 01:06:25,520
+المعمل .. المعمل ال microscopeرح نعمل
+
+864
+01:06:25,520 --> 01:06:30,340
+demonstration في بداية المعمل نشوف كيف احنا مع بعض
+
+865
+01:06:30,340 --> 01:06:33,940
+كيف ن .. نش .. نشبك ال microscope كيف بحضره للشغل
+
+866
+01:06:33,940 --> 01:06:38,260
+و كيف انا بجيب ال field و كيف بستخدمه و رح نتعرف
+
+867
+01:06:38,260 --> 01:06:41,720
+عن الأدوات أجزاء ال .. ال microscope عن قرب و كل
+
+868
+01:06:41,720 --> 01:06:45,640
+واحدة اش وظيفتها يعني إعادة بسيطة للمحاضرة أو
+
+869
+01:06:45,640 --> 01:06:52,140
+إفراء للمحاضرة على أرض الواقع يعطيكم العافية و
+
+870
+01:06:52,140 --> 01:06:53,320
+موفقين بإذن الله
+
diff --git a/PL9fwy3NUQKwY9jl8xw-bWkVZkWcGGyspd/Eut_8KsMs84_raw.json b/PL9fwy3NUQKwY9jl8xw-bWkVZkWcGGyspd/Eut_8KsMs84_raw.json
new file mode 100644
index 0000000000000000000000000000000000000000..e0a2f9311a832e2a7ab1337e351e45fdd016b461
--- /dev/null
+++ b/PL9fwy3NUQKwY9jl8xw-bWkVZkWcGGyspd/Eut_8KsMs84_raw.json
@@ -0,0 +1 @@
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مية، تمام؟ إذا إما بحبنعرفهم بقوة التكبير تبعتهم أو بpower تبعهم، هذه low power, high power, oil immersion", "tokens": [19528, 11703, 995, 5551, 5016, 4117, 1829, 23275, 1090, 1347, 10024, 37495, 22653, 47525, 4117, 6055, 14851, 9778, 40448, 18863, 3215, 3794, 3660, 5551, 25513, 3615, 9957, 12399, 5296, 15042, 5551, 5016, 4117, 1829, 23275, 3184, 40348, 10024, 37495, 22653, 18863, 3215, 3794, 3660, 3714, 10632, 12399, 46811, 10943, 22807, 11933, 15730, 11933, 15042, 4724, 5016, 3555, 1863, 3615, 28480, 16095, 4724, 4587, 2407, 3660, 16712, 4117, 3555, 13546, 6055, 3555, 34268, 16095, 34051, 4724, 9513, 6055, 3555, 3615, 16095, 12399, 29538, 2295, 1347, 11, 1090, 1347, 11, 3184, 40348], "avg_logprob": -0.17178915031663664, "compression_ratio": 1.625, "no_speech_prob": 1.5497207641601562e-06, "words": [{"start": 1139.03, "end": 1139.39, "word": "لمّا", "probability": 0.7975260416666666}, {"start": 1139.39, "end": 1140.13, "word": " أحكيلك", "probability": 0.93291015625}, {"start": 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"start": 2416.49, "end": 2438.87, "text": "طيب الضوء ينتقل من ال light source ممكن يمر بمرقاء او ب filter و بعدين بالقرص بيتحكم بكمية وجوده او كميته اللي فايتة ل ال condenser و ال condenser بيجمعه و بيخليه يعني يفوت في العدسة الشريقية اللي هي 100X او ال oil immersion", "tokens": [9566, 1829, 3555, 2423, 11242, 2407, 38207, 7251, 29399, 4587, 1211, 9154, 2423, 1442, 4009, 3714, 43020, 7251, 29973, 4724, 29973, 4587, 16606, 1975, 2407, 4724, 6608, 4032, 39182, 9957, 20666, 4587, 2288, 9381, 4724, 36081, 5016, 24793, 4724, 24793, 10632, 49610, 23328, 3224, 1975, 2407, 9122, 2304, 36081, 3224, 13672, 1829, 6156, 995, 36081, 3660, 5296, 2423, 2224, 30041, 4032, 2423, 2224, 30041, 4724, 1829, 7435, 2304, 3615, 3224, 4032, 4724, 1829, 9778, 20292, 3224, 37495, 22653, 7251, 5172, 35473, 8978, 18863, 3215, 3794, 3660, 25124, 16572, 4587, 10632, 13672, 1829, 39896, 2319, 55, 1975, 2407, 2423, 3184, 40348], "avg_logprob": -0.16104578971862793, "compression_ratio": 1.6164383561643836, 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3846.3, "word": " الكيس", "probability": 0.8958333333333334}, {"start": 3846.3, "end": 3847.08, "word": " أو", "probability": 0.213134765625}, {"start": 3847.08, "end": 3847.96, "word": " الحقيبة", "probability": 0.982421875}, {"start": 3847.96, "end": 3848.16, "word": " اللي", "probability": 0.94677734375}, {"start": 3848.16, "end": 3849.38, "word": " بتغطيه", "probability": 0.9732421875}], "temperature": 1.0}, {"id": 178, "seek": 386647, "start": 3849.71, "end": 3866.47, "text": "فإنه تكون عليها silica gel مادة silica gel وهي مادة drying agent عامل مجفف يمتص الرطوبة عشان العدسات ما تخرب وما ينمو عليها فطر والهيكل المعدني للميكروسكوب لا يصدق", "tokens": [5172, 28814, 1863, 3224, 6055, 30544, 25894, 11296, 3425, 2262, 4087, 3714, 18513, 3660, 3425, 2262, 4087, 37037, 1829, 3714, 18513, 3660, 22709, 9461, 6225, 10943, 1211, 3714, 7435, 5172, 5172, 7251, 2304, 2655, 9381, 34892, 9566, 37746, 3660, 6225, 8592, 7649, 18863, 3215, 3794, 9307, 19446, 6055, 9778, 25513, 4032, 15042, 7251, 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الشرايح انظف ال objective lenses و ال eye lenses و الcondenser طبعا كيف انا .. طبعا الcondenser يا بنات ممكن تتجمع عليه غبار عدسته فمهم جدا انك انتي تمسحيه قبل استخدامه", "tokens": [9566, 3555, 3615, 995, 1975, 4117, 25708, 412, 264, 917, 295, 1184, 786, 8978, 8717, 11296, 10632, 28242, 7251, 20498, 5296, 31377, 2304, 1975, 8315, 16472, 19913, 5172, 18863, 3215, 3794, 9307, 8032, 47302, 46811, 10943, 22807, 16472, 19913, 5172, 2423, 3233, 9154, 4724, 4587, 995, 25528, 6024, 110, 36081, 4032, 25124, 23557, 1829, 5016, 16472, 19913, 5172, 2423, 10024, 18059, 4032, 2423, 3313, 18059, 4032, 2423, 18882, 30041, 23032, 3555, 3615, 995, 9122, 33911, 1975, 8315, 4386, 23032, 3555, 3615, 995, 2423, 18882, 30041, 35186, 4724, 8315, 2655, 3714, 43020, 6055, 2655, 7435, 2304, 3615, 47356, 32771, 3555, 9640, 6225, 3215, 14851, 3224, 6156, 2304, 16095, 10874, 28259, 16472, 4117, 16472, 31371, 46811, 3794, 5016, 1829, 3224, 12174, 36150, 44713, 9778, 3215, 10943, 3224], "avg_logprob": 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الكحول إذا .. إلا إذا مش متوفر زايلين يعني فيفضل إن أنا أبعد عن الكحول لإن الكحول بسرع تلف العدسات", "tokens": [2407, 23032, 3555, 3615, 995, 9122, 33911, 44945, 19913, 5172, 16095, 22807, 44945, 19913, 5172, 16095, 4724, 15042, 41891, 3714, 9778, 9381, 9381, 3660, 5296, 11296, 13672, 1829, 39896, 24976, 11622, 995, 26895, 9957, 4032, 7251, 5172, 11242, 1211, 36145, 41850, 19446, 5551, 14851, 9778, 40448, 33251, 5016, 12610, 11933, 15730, 4386, 11933, 15040, 11933, 15730, 37893, 44650, 38688, 2288, 30767, 995, 26895, 9957, 37495, 22653, 6156, 33911, 11242, 1211, 36145, 41850, 5551, 3555, 22488, 18871, 33251, 5016, 12610, 5296, 28814, 1863, 33251, 5016, 12610, 4724, 3794, 2288, 3615, 6055, 46538, 18863, 3215, 3794, 9307], "avg_logprob": -0.1162109380432715, "compression_ratio": 1.8837209302325582, "no_speech_prob": 1.5079975128173828e-05, "words": [{"start": 3891.42, "end": 3891.74, "word": "و", "probability": 0.9580078125}, {"start": 3891.74, "end": 3892.78, "word": " طبعا", 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.. نش .. نشبك ال microscope كيف بحضره للشغل و كيف انا بجيب ال field و كيف بستخدمه و رح نتعرف عن الأدوات أجزاء ال .. ال microscope عن قرب و كل واحدة اش وظيفتها يعني إعادة بسيطة للمحاضرة أو إفراء للمحاضرة على أرض الواقع يعطيكم العافية و موفقين بإذن الله", "tokens": [2288, 5016, 8717, 25957, 1211, 16520, 8978, 4724, 28259, 10632, 9673, 25957, 1211, 8717, 8592, 38688, 9122, 33911, 1975, 5016, 8315, 20449, 45030, 11242, 9122, 33911, 8717, 4386, 8717, 8592, 4386, 8717, 8592, 3555, 4117, 2423, 29753, 9122, 33911, 4724, 5016, 11242, 2288, 3224, 24976, 8592, 17082, 1211, 4032, 9122, 33911, 1975, 8315, 4724, 7435, 1829, 3555, 2423, 2519, 4032, 9122, 33911, 4724, 14851, 9778, 40448, 3224, 4032, 12602, 5016, 8717, 2655, 3615, 28480, 18871, 16247, 3215, 2407, 9307, 5551, 7435, 11622, 16606, 2423, 4386, 2423, 29753, 18871, 12174, 25513, 4032, 28242, 36764, 24401, 3660, 1975, 8592, 4032, 19913, 33911, 2655, 11296, 37495, 22653, 11933, 3615, 18513, 3660, 4724, 3794, 1829, 9566, 3660, 5296, 19528, 5016, 46958, 25720, 34051, 11933, 5172, 2288, 16606, 5296, 19528, 5016, 46958, 25720, 15844, 5551, 43042, 2423, 14407, 4587, 3615, 37495, 9566, 1829, 24793, 18863, 31845, 10632, 4032, 3714, 38688, 4587, 9957, 4724, 28814, 8848, 1863, 21984], "avg_logprob": -0.11080473778294582, "compression_ratio": 1.7918088737201365, "no_speech_prob": 2.282857894897461e-05, "words": [{"start": 3984.86, "end": 3985.16, "word": "رح", "probability": 0.707275390625}, {"start": 3985.16, "end": 3985.52, "word": " نعمل", "probability": 0.9959309895833334}, {"start": 3985.52, "end": 3986.4, "word": " demonstration", "probability": 0.775390625}, {"start": 3986.4, "end": 3986.8, "word": " في", "probability": 0.75390625}, {"start": 3986.8, "end": 3987.2, "word": " بداية", "probability": 0.9777018229166666}, {"start": 3987.2, "end": 3987.78, "word": " المعمل", "probability": 0.9944661458333334}, {"start": 3987.78, "end": 3988.98, "word": " نشوف", "probability": 0.9189453125}, {"start": 3988.98, "end": 3989.22, "word": " كيف", "probability": 0.957763671875}, {"start": 3989.22, "end": 3989.58, "word": " احنا", "probability": 0.8927408854166666}, {"start": 3989.58, "end": 3989.92, "word": " مع", "probability": 0.9873046875}, {"start": 3989.92, "end": 3990.34, "word": " بعض", "probability": 0.9951171875}, {"start": 3990.34, "end": 3990.84, "word": " كيف", "probability": 0.85400390625}, {"start": 3990.84, "end": 3991.04, "word": " ن", "probability": 0.425048828125}, {"start": 3991.04, "end": 3991.26, "word": " ..", "probability": 0.9150390625}, {"start": 3991.26, "end": 3991.54, "word": " نش", "probability": 0.806396484375}, {"start": 3991.54, "end": 3991.64, "word": " ..", "probability": 0.306396484375}, {"start": 3991.64, "end": 3992.12, "word": " نشبك", "probability": 0.9112548828125}, {"start": 3992.12, "end": 3992.22, "word": " ال", "probability": 0.88232421875}, {"start": 3992.22, "end": 3992.66, "word": " microscope", "probability": 0.57568359375}, {"start": 3992.66, "end": 3992.94, "word": " كيف", "probability": 0.90185546875}, {"start": 3992.94, "end": 3993.4, "word": " بحضره", "probability": 0.944140625}, {"start": 3993.4, "end": 3993.94, "word": " للشغل", "probability": 0.9259033203125}, {"start": 3993.94, "end": 3994.1, "word": " و", "probability": 0.97314453125}, {"start": 3994.1, "end": 3994.4, "word": " كيف", "probability": 0.885986328125}, {"start": 3994.4, "end": 3994.66, "word": " انا", "probability": 0.7421875}, {"start": 3994.66, "end": 3995.02, "word": " بجيب", "probability": 0.9925537109375}, {"start": 3995.02, "end": 3995.16, "word": " ال", "probability": 0.96240234375}, {"start": 3995.16, "end": 3995.46, "word": " field", "probability": 0.96875}, {"start": 3995.46, "end": 3995.66, "word": " و", "probability": 0.9814453125}, {"start": 3995.66, "end": 3995.98, "word": " كيف", "probability": 0.931396484375}, {"start": 3995.98, "end": 3996.76, "word": " بستخدمه", "probability": 0.973046875}, {"start": 3996.76, "end": 3997.66, "word": " و", "probability": 0.84814453125}, {"start": 3997.66, "end": 3997.84, "word": " رح", "probability": 0.817138671875}, {"start": 3997.84, "end": 3998.26, "word": " نتعرف", "probability": 0.9959716796875}, {"start": 3998.26, "end": 3998.46, "word": " عن", "probability": 0.92919921875}, {"start": 3998.46, "end": 3998.94, "word": " الأدوات", "probability": 0.821533203125}, {"start": 3998.94, "end": 3999.92, "word": " أجزاء", "probability": 0.837646484375}, {"start": 3999.92, "end": 4000.18, "word": " ال", "probability": 0.98876953125}, {"start": 4000.18, "end": 4000.18, "word": " ..", "probability": 0.9482421875}, {"start": 4000.18, "end": 4000.34, "word": " ال", "probability": 0.89697265625}, {"start": 4000.34, "end": 4000.86, "word": " microscope", "probability": 0.90234375}, {"start": 4000.86, "end": 4001.14, "word": " عن", "probability": 0.943359375}, {"start": 4001.14, "end": 4001.44, "word": " قرب", "probability": 0.981689453125}, {"start": 4001.44, "end": 4001.58, "word": " و", "probability": 0.99267578125}, {"start": 4001.58, "end": 4001.72, "word": " كل", "probability": 0.85400390625}, {"start": 4001.72, "end": 4002.0, "word": " واحدة", "probability": 0.97216796875}, {"start": 4002.0, "end": 4002.12, "word": " اش", "probability": 0.657958984375}, {"start": 4002.12, "end": 4002.8, "word": " وظيفتها", "probability": 0.9666015625}, {"start": 4002.8, "end": 4004.02, "word": " يعني", "probability": 0.940673828125}, {"start": 4004.02, "end": 4004.44, "word": " إعادة", "probability": 0.927978515625}, {"start": 4004.44, "end": 4004.86, "word": " بسيطة", "probability": 0.99697265625}, {"start": 4004.86, "end": 4005.48, "word": " للمحاضرة", "probability": 0.9283203125}, {"start": 4005.48, "end": 4005.64, "word": " أو", "probability": 0.689453125}, {"start": 4005.64, "end": 4006.04, "word": " إفراء", "probability": 0.8299560546875}, {"start": 4006.04, "end": 4007.04, "word": " للمحاضرة", "probability": 0.96474609375}, {"start": 4007.04, "end": 4008.7, "word": " على", "probability": 0.9169921875}, {"start": 4008.7, "end": 4009.04, "word": " أرض", "probability": 0.9765625}, {"start": 4009.04, "end": 4009.56, "word": " الواقع", "probability": 0.983642578125}, {"start": 4009.56, "end": 4010.78, "word": " يعطيكم", "probability": 0.8712158203125}, {"start": 4010.78, "end": 4011.64, "word": " العافية", "probability": 0.98974609375}, {"start": 4011.64, "end": 4012.14, "word": " و", "probability": 0.99365234375}, {"start": 4012.14, "end": 4012.76, "word": " موفقين", "probability": 0.9256591796875}, {"start": 4012.76, "end": 4013.08, "word": " بإذن", "probability": 0.843505859375}, {"start": 4013.08, "end": 4013.32, "word": " الله", "probability": 0.9443359375}], "temperature": 1.0}], "language": "ar", "language_probability": 1.0, "duration": 4014.79975, "duration_after_vad": 3927.640624999989}
\ No newline at end of file
diff --git a/PL9fwy3NUQKwY9jl8xw-bWkVZkWcGGyspd/FJ1Ngmal9to_postprocess.srt b/PL9fwy3NUQKwY9jl8xw-bWkVZkWcGGyspd/FJ1Ngmal9to_postprocess.srt
new file mode 100644
index 0000000000000000000000000000000000000000..57b6420fbb7295a876dbd4cb4303fd1e6a206c60
--- /dev/null
+++ b/PL9fwy3NUQKwY9jl8xw-bWkVZkWcGGyspd/FJ1Ngmal9to_postprocess.srt
@@ -0,0 +1,2264 @@
+1
+00:00:06,630 --> 00:00:10,490
+السلام عليكم ورحمة الله وبركاته طالبات العزيزات
+
+2
+00:00:10,490 --> 00:00:15,050
+اليوم ان شاء الله راح نشرح محاضرة بعنوان red blood
+
+3
+00:00:15,050 --> 00:00:19,530
+cell count RBC count في نهاية هذه المحاضرة راح
+
+4
+00:00:19,530 --> 00:00:25,070
+نكون قادرين بإذن الله انه نتعرف على طريقة لعد
+
+5
+00:00:25,070 --> 00:00:31,310
+خلايا الدمطريقة manual يدوية إنه كيف بنعد خلايا
+
+6
+00:00:31,310 --> 00:00:37,310
+الدم الحمراء باستخدام ال chamber شريحة ومخصصة لعد
+
+7
+00:00:37,310 --> 00:00:41,930
+الخلايا وباستخدام ال microscope وكيف بنعمل
+
+8
+00:00:41,930 --> 00:00:45,370
+dilution ل blood لحتى نتمكن من عد خلايا الدم
+
+9
+00:00:45,370 --> 00:00:49,740
+الحمراءمعلومات مشابهة لما ذكرناها بال white blood
+
+10
+00:00:49,740 --> 00:00:55,340
+cell count ولكن مع اختلاف في بعض التفاصيل خلينا في
+
+11
+00:00:55,340 --> 00:01:02,300
+البداية نذكر كتقديم بسيط للموضوع نذكر أهمية ال ..
+
+12
+00:01:02,300 --> 00:01:08,020
+ال red blood cells ووظائفهاRed blood cell make up
+
+13
+00:01:08,020 --> 00:01:14,540
+almost 45% of the blood volume تقريبا تحت الخلايا
+
+14
+00:01:14,540 --> 00:01:19,980
+الدم الحمراء حجم 45% من حجم الدم ككل في بعض
+
+15
+00:01:19,980 --> 00:01:24,780
+المراجع ذكرنا أنه 44% على أساس أن ال buffy coat 1%
+
+16
+00:01:25,150 --> 00:01:32,050
+طبعا مش خلال 45% او 44% المهم انها بتحتل جزء ..
+
+17
+00:01:32,050 --> 00:01:38,790
+خلينا نحكي تقريبا 45% من حجم الدم ككل their
+
+18
+00:01:38,790 --> 00:01:44,450
+primary function is to carry oxygen وظيفة الخلايا
+
+19
+00:01:44,450 --> 00:01:49,830
+الدم الحمراء الأساسية طبعا انها تحمل ال oxygen من
+
+20
+00:01:49,830 --> 00:01:54,010
+وين؟ من ال lungلوين؟ to the every cell in the body
+
+21
+00:01:54,010 --> 00:01:58,310
+ل البلد tissue ل البلد organs بتوصل ال oxygen
+
+22
+00:01:58,310 --> 00:02:03,290
+تمام؟ Red blood cell الخلايا الدم الحمراء composed
+
+23
+00:02:03,290 --> 00:02:07,650
+predominantly of a protein and iron compound طبعا
+
+24
+00:02:07,650 --> 00:02:15,930
+هي الجزء او المساحة العظمى من ال RBCs موجود فيها
+
+25
+00:02:15,930 --> 00:02:20,570
+او بيحتلها homoglobinموجود فيها بروتين هو عبارة عن
+
+26
+00:02:20,570 --> 00:02:26,210
+iron containing بروتين بروتين يحمل ذرات ال iron
+
+27
+00:02:26,210 --> 00:02:30,630
+هذا البروتين هو ال homoglobin ال homoglobin هو
+
+28
+00:02:30,630 --> 00:02:35,830
+الجزء او البروتين المسئول عن حمل ال oxygen و نقله
+
+29
+00:02:35,830 --> 00:02:42,150
+حكينا انه من خصائصه انه بيحمل او بيتلقى oxygen في
+
+30
+00:02:42,150 --> 00:02:45,690
+المناطق اللي بيكون فيها ال oxygen pressure عالي زي
+
+31
+00:02:45,690 --> 00:02:51,090
+ال lung تمام؟وبيترك ال oxygen release تمام؟ يترك
+
+32
+00:02:51,090 --> 00:02:54,690
+ال oxygen أو release ال oxygen في المناطق اللي
+
+33
+00:02:54,690 --> 00:02:58,590
+بيكون فيها ال oxygen pressure منخفض زي ان ال
+
+34
+00:02:58,590 --> 00:03:01,610
+tissues و ال organs و ال blood cells every blood
+
+35
+00:03:01,610 --> 00:03:07,740
+cells تمام؟ال .. ال homoglobin هو اللي لونه أحمر
+
+36
+00:03:07,740 --> 00:03:13,640
+لإنه بيحتوي على ال iron تمام فبالتالي هو اللي يكسب
+
+37
+00:03:13,640 --> 00:03:18,540
+خلايا الدم لونها الأحمر يكسب خلايا الدم الحمراء
+
+38
+00:03:18,540 --> 00:03:23,620
+طبعا لونها الأحمر the blood vessels through body
+
+39
+00:03:23,620 --> 00:03:27,760
+tissueshomoglobin then release the oxygen to the
+
+40
+00:03:27,760 --> 00:03:31,840
+cell or the body حكينا إنه هو بيحمل ال oxygen من
+
+41
+00:03:31,840 --> 00:03:37,820
+ال lung تمام أثناء مرور الدم عبر الرقا تمام بيحمل
+
+42
+00:03:37,820 --> 00:03:41,920
+ال oxygen لإن ضغط ال oxygen في ال lung عاليو as
+
+43
+00:03:41,920 --> 00:03:46,120
+blood pass through the body لما ال .. ال .. الدم
+
+44
+00:03:46,120 --> 00:03:51,360
+بي .. بي circulate طبعا، بي .. بيدور .. بي ..
+
+45
+00:03:51,360 --> 00:03:54,640
+بينتقل الى ال tissue و بيروح يغذي ال tissue يعنى،
+
+46
+00:03:54,640 --> 00:04:00,520
+طبعا فبالتالي هو في هذه المرحلة بيوصل oxygen، it
+
+47
+00:04:00,520 --> 00:04:05,100
+releasethe oxygen، تمام؟ red blood cells are so
+
+48
+00:04:05,100 --> 00:04:09,000
+packed with homoglobin، حكينا إنها هي معبقة،
+
+49
+00:04:09,000 --> 00:04:14,640
+مملوقة بالهمoglobin، بيشغل حجم كتير كبير من الحجم
+
+50
+00:04:14,640 --> 00:04:18,740
+الكل لخلية الدم الحمراء، ال homoglobinincluding
+
+51
+00:04:18,740 --> 00:04:23,740
+طبعا هذا بيخلي ال RBCs lack .. lack money
+
+52
+00:04:23,740 --> 00:04:28,220
+component طبعا تفتقر او انها تفتقد كثير من
+
+53
+00:04:28,220 --> 00:04:32,640
+المكونات اللي موجودة في الخلايا الأخرى او الخلايا
+
+54
+00:04:32,640 --> 00:04:36,900
+الطبيعية العادية يعني مثلا خلية الدم الحمراء
+
+55
+00:04:36,900 --> 00:04:41,160
+ماعندهاش nucleus ماعندهاش organelles طبعا؟ ليش؟
+
+56
+00:04:41,160 --> 00:04:45,640
+لإن لحتىاتمكنها من حمل كمية كافية من ال
+
+57
+00:04:45,640 --> 00:04:49,360
+homoglobin، مافيش nucleus، مافيش organelles علشان
+
+58
+00:04:49,360 --> 00:04:55,760
+الخلية تحمل homoglobin بالقدر الكافي اللي يمكنها
+
+59
+00:04:55,760 --> 00:05:00,940
+من توصيل ال oxygen إلى كل خلية داخل جسم الإنسان
+
+60
+00:05:02,620 --> 00:05:07,720
+RBCs structure the membrane or out layer of the
+
+61
+00:05:07,720 --> 00:05:11,560
+red blood cell is flexible تمام؟ Like a soup
+
+62
+00:05:11,560 --> 00:05:16,760
+bubble هلأ الخلايا أو ال membrane، الغلاف تبعي
+
+63
+00:05:16,760 --> 00:05:23,520
+خلايا ال RBCsهو غلاف مرن جدا، flexible، تمام؟ مرن
+
+64
+00:05:23,520 --> 00:05:27,720
+جدا، يعني بيشبهوها بفقاعة صابون، شوفنا كيف لعبة
+
+65
+00:05:27,720 --> 00:05:31,240
+الفقاعات هاي اللي بتطلع منها الفقاعة من حلقة
+
+66
+00:05:31,240 --> 00:05:35,610
+دائرية، بنحط ميه و صابون في العلبة و ..و من خلال
+
+67
+00:05:35,610 --> 00:05:38,850
+النفخ عليها بتطلع فقاعة صبون شفتوا كيف فقاعة
+
+68
+00:05:38,850 --> 00:05:42,710
+الصبون بالأول بتتشكل و بتنحني لحد بعد ما تطلع من
+
+69
+00:05:42,710 --> 00:05:46,810
+الإطار ال RBCs زي هات يعني نفس .. بيشبهوها نفس
+
+70
+00:05:46,810 --> 00:05:51,810
+المرونة ليش؟ لإن ال RBCs bend able to bend ممكن
+
+71
+00:05:51,810 --> 00:05:56,890
+إنها تنحني أو تلتف على نفسها أو يعني يصير فيها
+
+72
+00:05:56,890 --> 00:06:02,420
+انثناقات تمام؟و بدون ما تنكسر without breaking
+
+73
+00:06:02,420 --> 00:06:06,220
+طبعا .. without breaking وين طبعا بيصير فيها هاي
+
+74
+00:06:06,220 --> 00:06:10,320
+الانحناقات و السهولة
+
+75
+00:06:10,320 --> 00:06:14,120
+و المرونة فى الحركة فى لما بدها تفوت فى ال blood
+
+76
+00:06:14,120 --> 00:06:16,860
+vessels اللى حجمها صغير فى ال capillaries الشعيرات
+
+77
+00:06:16,860 --> 00:06:20,500
+الدموية الدقيقة جدا لما بتفوت أحيانا تضطر ان هي
+
+78
+00:06:20,500 --> 00:06:25,100
+تتنحالها علشان تروح توصل oxygen طبعا او انها ..
+
+79
+00:06:25,100 --> 00:06:29,720
+يعني يصير فيها انحناقات تلتوي تلتف على نفسهافي
+
+80
+00:06:29,720 --> 00:06:34,180
+أيضًا غير الأوعية الدموية الضيقة على التفافات
+
+81
+00:06:34,180 --> 00:06:37,360
+والانحناقات
+
+82
+00:06:37,360 --> 00:06:41,840
+اللي موجودة أيضًا في الأوعية الدموية برضه كمان
+
+83
+00:06:41,840 --> 00:06:47,980
+بتكون ال RBCs قادرة على أنها تفني نفسها و تفوت
+
+84
+00:06:47,980 --> 00:06:51,400
+لحتى توصل أكسوجين everywhere this is important
+
+85
+00:06:51,400 --> 00:06:56,330
+طبعًا هي خاصية كتير مهمة في خلايا الدم الحمراءليش؟
+
+86
+00:06:56,330 --> 00:07:00,050
+لإن هي بتمكنها able to pass through the thinnest
+
+87
+00:07:00,050 --> 00:07:05,710
+blood vessels هي بتمكنها من إنها تتعبر من خلال
+
+88
+00:07:05,710 --> 00:07:09,730
+يعني أدق خلاء الأوعية الدموية thinnest blood
+
+89
+00:07:09,730 --> 00:07:12,190
+vessels يعني أدق الأوعية الدموية اللي هي
+
+90
+00:07:12,190 --> 00:07:16,310
+الكابيلاريز الشعيرات طبعا لحتى توصل ال oxygen to
+
+91
+00:07:16,310 --> 00:07:19,910
+deliver oxygen whenever it is needed في أي مكان
+
+92
+00:07:19,910 --> 00:07:24,400
+احنا محتاجينه طبعا بتوصل هذا ال oxygenخلّونا بس
+
+93
+00:07:24,400 --> 00:07:29,700
+برضه كمان نربط خاصية ال flexibility بال fragility
+
+94
+00:07:29,700 --> 00:07:35,700
+لل RBCs هي أي نعم ال RBCs مرنة و بإمكانها إنها هي
+
+95
+00:07:35,700 --> 00:07:39,740
+تلتف على نفسها يصير في انحناقات انثناقات في ال
+
+96
+00:07:39,740 --> 00:07:46,780
+membrane و تروح يعني تتعبر نفسها أو تتمرر نفسها من
+
+97
+00:07:46,780 --> 00:07:51,260
+خلال capillaries ضيقة جدا لحتى توصي ال oxygen إلا
+
+98
+00:07:51,260 --> 00:07:56,980
+إنهأيضًا الخلايا الـ RBCs تُعتبر إلى حد ما .. إلى
+
+99
+00:07:56,980 --> 00:08:02,100
+حد ما تُعتبر fragile تُعتبر هشة سهلة الكسر لهيك
+
+100
+00:08:02,100 --> 00:08:05,840
+إحنا لما بنحكيلكم اصحى بيعين الدم عوّلي تعملي
+
+101
+00:08:05,840 --> 00:08:10,220
+gentle mixing ديري بالك الخلايا تتكسر لإنها
+
+102
+00:08:10,220 --> 00:08:16,020
+mechanicalي مع الحركة القوية أو مع السوق التعامل
+
+103
+00:08:16,020 --> 00:08:20,870
+ممكن إنها أيضًا تتكسر هاي الخلاياthe capillaries
+
+104
+00:08:20,870 --> 00:08:25,250
+are so narrow تمام الأوعية الدموية الضيقة هاي the
+
+105
+00:08:25,250 --> 00:08:29,230
+thinnest blood vessels الأشعيرات الدموية are so
+
+106
+00:08:29,230 --> 00:08:35,030
+narrow ضيقة جدا to the limit that لحد ان ال RBCs
+
+107
+00:08:35,030 --> 00:08:38,610
+اللي بشكلها الطبيعي عاملة زي القرص او زي ال bacon
+
+108
+00:08:38,610 --> 00:08:45,330
+cap shape لازم انها must bend and twist to pass
+
+109
+00:08:45,330 --> 00:08:49,810
+through them علشان تتعدي من خلالهم تمام؟bend and
+
+110
+00:08:49,810 --> 00:08:54,250
+twist معناها إنها يعني تنحني وتلتف، هيك معناها،
+
+111
+00:08:54,250 --> 00:08:58,050
+طبعا، طيب الشكل الطبيعي لل RBCs إذا أنا جيت ألقيت
+
+112
+00:08:58,050 --> 00:09:02,250
+عليها نظرة علوية، طبعا، لو كانت ال RBCs موجودة على
+
+113
+00:09:02,250 --> 00:09:05,790
+شريحة و أنا جيت و طلعت عليها من فوق، بشوفها ..
+
+114
+00:09:05,790 --> 00:09:08,470
+بشوف ال top view تبعها إن هي عبارة عن زي ال discs
+
+115
+00:09:08,470 --> 00:09:13,250
+زي القرص، طبعا، بنشوفها circular أو discotheque،
+
+116
+00:09:13,250 --> 00:09:16,850
+طبعاcircular أو discotheque خلينا نكتب كلمة
+
+117
+00:09:16,850 --> 00:09:19,530
+discotheque عشان ما تكونش غريبة عليكم،
+
+118
+00:09:19,530 --> 00:09:27,890
+discotheque، تمام؟ أو بنسميها disc
+
+119
+00:09:27,890 --> 00:09:33,810
+shape، تمام؟ زي شكل القرص، discotheque أو disc
+
+120
+00:09:33,810 --> 00:09:39,490
+shapeطيب انا لو جيت اطلعت من الجانب على خلايا ال
+
+121
+00:09:39,490 --> 00:09:43,470
+RBC's ايش بشوفها؟ طب لو ان احنا جينا هنا .. انتوا
+
+122
+00:09:43,470 --> 00:09:47,550
+شايفين هنا زي في .. زي كأنه حفرة او ايشي ضيق ..
+
+123
+00:09:47,550 --> 00:09:50,870
+ايشي داخل لجوا في الخلية لو جيت انا اطلعت من
+
+124
+00:09:50,870 --> 00:09:54,690
+الجانب من هنابشوف في عندي انتثاح و انتثاح و في
+
+125
+00:09:54,690 --> 00:09:59,450
+المصمنطقة ضيقة اللي هي بسميها biconcave shape
+
+126
+00:09:59,450 --> 00:10:04,730
+مقعرة الوجهين، من هنا مقعر و من هنا مقعر، تمام؟
+
+127
+00:10:04,730 --> 00:10:09,930
+إذا مقعرة الوجهين، هي مقعر و أيضا مقعر آخر فبسميها
+
+128
+00:10:09,930 --> 00:10:15,960
+biconcave shape، تمام؟إذا ال top view، النظرة
+
+129
+00:10:15,960 --> 00:10:19,800
+العلوية لل RBCs بشوفها circular، بشوفها
+
+130
+00:10:19,800 --> 00:10:24,760
+discotheque، disc shape تمام؟ أما النظرة الجانبية
+
+131
+00:10:24,760 --> 00:10:30,820
+لل RBCs فبشوفها عبارة عن biconcave تمام؟ بشوفها
+
+132
+00:10:30,820 --> 00:10:36,380
+biconcave يعني مقعرة الوجهين concave top تمام؟ and
+
+133
+00:10:36,380 --> 00:10:43,780
+bottomطيب Erythrocyte count طبعا أنا ب .. ب ..
+
+134
+00:10:43,780 --> 00:10:49,940
+يعني بعدي الخلايا per microliter تمام؟ يعني وحدة
+
+135
+00:10:49,940 --> 00:10:55,180
+القياس سل per microliter أو سل per millimeter مكعب
+
+136
+00:10:55,180 --> 00:10:57,840
+و هذا الكلام ذكرناه في محاضرة ال white pieces
+
+137
+00:10:57,840 --> 00:11:03,870
+countنيجي لل normal ranges of RBCs المال الوضره
+
+138
+00:11:03,870 --> 00:11:09,930
+الطبيعى من 4.2 ل 5.4 مليون خلية حكينا عددها
+
+139
+00:11:09,930 --> 00:11:16,890
+بالملايين ال female 3.6 ل 5 و ال newborn 5.5 ل 6.5
+
+140
+00:11:16,890 --> 00:11:20,250
+طبعا صيرنا بنعرف ليش ال newborn عنده خلايا RBCs
+
+141
+00:11:20,250 --> 00:11:24,150
+اكبر او اكثر و صيرنا بنعرف ليش المال عنده خلايا
+
+142
+00:11:24,150 --> 00:11:28,650
+RBCs اكثر من ال female تمام؟هذه عوامل أو أسباب
+
+143
+00:11:28,650 --> 00:11:33,550
+احنا ذكرناها سابقا وصرنا ايضا بنعرف ان زيادة
+
+144
+00:11:33,550 --> 00:11:38,210
+الارثروسايت count تمام لما يكون عندى زيادة فى عدد
+
+145
+00:11:38,210 --> 00:11:43,410
+ال RBCs زيادة فى حجم ال RBCs ال hematocrit زيادة
+
+146
+00:11:43,410 --> 00:11:46,510
+فى ال homoglobin هذه كلها بسميها polycythemia
+
+147
+00:11:47,190 --> 00:11:52,870
+مؤشرات على polycythemia أما نقص عدد ال RBCs، نقص
+
+148
+00:11:52,870 --> 00:11:55,790
+ال hematocrit اللي هو الحجم اللي تحتله ال RBCs،
+
+149
+00:11:55,790 --> 00:12:00,750
+نقص ال hemoglobin بسميه أنيميا، تمام؟ بسميه أنيميا
+
+150
+00:12:00,750 --> 00:12:04,830
+يعني فقرة دم، وحكينا إن فيلة ال polycythemia حالات
+
+151
+00:12:04,830 --> 00:12:11,900
+و أيضا فيلة الأنيميا أنواعطيب erythrocyte count
+
+152
+00:12:11,900 --> 00:12:16,660
+the principle مبدأ عمل ال erythrocyte count in
+
+153
+00:12:16,660 --> 00:12:21,040
+order to facilitate RBCs count a specified volume
+
+154
+00:12:21,040 --> 00:12:24,800
+of blood is diluted with a specified volume of
+
+155
+00:12:24,800 --> 00:12:31,180
+isotonic fluidطبعا أنا بدي أاخد حجم من الدم طبعا و
+
+156
+00:12:31,180 --> 00:12:37,220
+holy blood لإنه أنا بشتغل على الخلايا هلأ و لازم
+
+157
+00:12:37,220 --> 00:12:42,800
+أخففه بحجم معين من ال daily one نفس فكرة ال white
+
+158
+00:12:42,800 --> 00:12:47,420
+pieces countتمام؟ وكنت باخد حجم من الدم و باخد حجم
+
+159
+00:12:47,420 --> 00:12:51,140
+من ال diligent طيب خصائص ال diligent كان في ال
+
+160
+00:12:51,140 --> 00:12:56,940
+white pieces count انه لازم يكسرلي ال .. ال RPCs و
+
+161
+00:12:56,940 --> 00:13:02,140
+يحفظلي ال .. ال white pieces و يصبغها علشان أشوفها
+
+162
+00:13:02,140 --> 00:13:08,360
+تمام؟ لكن هنا أنا صح بعمل dilution باخد حجم من ال
+
+163
+00:13:08,360 --> 00:13:14,650
+blood تمام؟ و حجم من ال diligent ولكنلازم يكون ال
+
+164
+00:13:14,650 --> 00:13:19,490
+diligent isotonic fluid وطبعا احنا في محاضرة من
+
+165
+00:13:19,490 --> 00:13:23,450
+المحاضرات فرقنا اش يعني hypotonic؟ اش يعني
+
+166
+00:13:23,450 --> 00:13:27,250
+hypertonic؟ واش يعني isotonic؟ وحكينا ان ال
+
+167
+00:13:27,250 --> 00:13:31,570
+isotonic هو اللي بيحافظ على شكل الخلايا مابيخليهاش
+
+168
+00:13:31,570 --> 00:13:37,050
+تتكسر يصير لها lysis او swelling او حتى كمان
+
+169
+00:13:37,050 --> 00:13:41,340
+مابيصيرلهاش shrinkageتمام؟ فإذا هو ال .. ال
+
+170
+00:13:41,340 --> 00:13:44,840
+diligent لازم يكون من خصائص ان هو isotonic يعني ال
+
+171
+00:13:44,840 --> 00:13:47,700
+.. الضغط الإسموزي تبعه مضبوط يحافظ على ضغط
+
+172
+00:13:47,700 --> 00:13:51,340
+الإسموزي للخلايا تمام؟ فبالتالي نسبة الأملح فيه
+
+173
+00:13:51,340 --> 00:13:55,580
+مضبوطة حتى .. حتى يحافظ على ضغط الإسموزي للخلايا
+
+174
+00:13:55,580 --> 00:14:02,250
+وبالتالي يحافظ على شكل الخلاياأيضا هناك شروط أخرى
+
+175
+00:14:02,250 --> 00:14:07,030
+لازم تتوفر في red cell diluting fluid إيش هي
+
+176
+00:14:07,030 --> 00:14:10,790
+الشروط اللي لازم تتوفر في ال diluent اللي أنا بدي
+
+177
+00:14:10,790 --> 00:14:15,230
+أستخدمه لعد خلايا ال red blood cells أول حاجة لازم
+
+178
+00:14:15,230 --> 00:14:20,140
+يكون anticoagulantيمنع تخسر الدم لإن إذا تخسر الدم
+
+179
+00:14:20,140 --> 00:14:25,060
+.. إذا تخسر الدم رح يصير في عندي clotting تجمع
+
+180
+00:14:25,060 --> 00:14:29,400
+للدم .. تجمع لل RBC عاملة زي clot زي كتلة و
+
+181
+00:14:29,400 --> 00:14:34,420
+بالتالي جلطة يعني فبالتالي هيك استهلاك عدد من
+
+182
+00:14:34,420 --> 00:14:38,460
+خلايا ال red blood cell صارلها trapping في ال clot
+
+183
+00:14:38,460 --> 00:14:43,180
+و يعني انحجزت في ال clot فبالتالي هيك رح يقلل عدد
+
+184
+00:14:43,180 --> 00:14:48,180
+خلايا الدم لل RBC ويعطيني error تمام؟لازم كمان
+
+185
+00:14:48,180 --> 00:14:51,800
+يكون antihemolysis يعني إيه إيش antihemolysis؟
+
+186
+00:14:51,800 --> 00:14:56,080
+يمنع تكسر الدم، يعني isotonic. Anti aggregation
+
+187
+00:14:56,080 --> 00:14:59,640
+يمنع تجمع خلاهي ال RBCs جنب بعضها البعض أو
+
+188
+00:14:59,640 --> 00:15:02,740
+تقاربها، لازم تكون هيك بعيدة عن بعضها البعض عشان
+
+189
+00:15:02,740 --> 00:15:08,550
+أنا أتمكن من عدهاAnti-relaw أيضًا ما يمنع تكدس ال
+
+190
+00:15:08,550 --> 00:15:12,210
+RBCs على بعضها البعض ويعرفنا إيش يعني كلمة relaw
+
+191
+00:15:12,210 --> 00:15:18,190
+يعني stacks like coins صح؟ أنه تلتثق ببعضها البعض
+
+192
+00:15:18,190 --> 00:15:22,030
+زي القطع المعدنية وغلط هذا طب أنا كده أعدها لازم
+
+193
+00:15:22,030 --> 00:15:26,370
+تكون منفصلة علشان أعدها لازم أيضًا من شروطه أنه
+
+194
+00:15:26,370 --> 00:15:31,870
+يكون ب reserve RBCs ship تمام؟ أنه يحافظ على شكل
+
+195
+00:15:31,870 --> 00:15:36,300
+ال RBCs اللي أتمكن من عدهافي عندي خيارات تمام
+
+196
+00:15:36,300 --> 00:15:40,860
+isotonic solution اللي هو isotonic saline و اتذكر
+
+197
+00:15:40,860 --> 00:15:43,980
+ان يعني ا .. ان احنا ذكرنا في محاضرة ال ESR ذكرنا
+
+198
+00:15:43,980 --> 00:15:47,200
+ال saline حكينا محلول ملحي المحلول الملحي المعقم
+
+199
+00:15:47,200 --> 00:15:51,380
+اللي انا بشتريه من الصيدلية لتعقيم جرح ل .. احيانا
+
+200
+00:15:51,380 --> 00:15:56,920
+برضه كمان بيحطوه ك .. ك .. في الوريد يعني كغداء او
+
+201
+00:15:56,920 --> 00:16:00,770
+يعني لحتى يرفع مثلا ..حجم الدم الناس اللي ضغطها
+
+202
+00:16:00,770 --> 00:16:03,870
+وعطي الناس اللي مغمة عليها كذا ممكن يحطولها إياها
+
+203
+00:16:03,870 --> 00:16:07,510
+ده محلول ملحي موجود فيه ملح الطعام Sodium Chloride
+
+204
+00:16:07,510 --> 00:16:12,930
+بس بنسبة معينة بنسبة 85% تمام؟ وبالتالي
+
+205
+00:16:14,890 --> 00:16:20,210
+كمية الأملح هذه مضبوطة على إنها تحافظ على الضغط
+
+206
+00:16:20,210 --> 00:16:23,690
+الأسموزي للخلايا فبالتالي هو Anti-Coagulant, Anti
+
+207
+00:16:23,690 --> 00:16:25,990
+-Hemolysis, Anti-Aggregation, Anti-Rouleau, We
+
+208
+00:16:25,990 --> 00:16:31,670
+preserve RBCs count أيضًا في عندي من المحليل
+
+209
+00:16:31,670 --> 00:16:36,290
+Hayam's solution تمام؟ Hayam's solution مكون من
+
+210
+00:16:36,290 --> 00:16:40,860
+Sodium Chlorideو ال sodium chloride عشان يحافظ على
+
+211
+00:16:40,860 --> 00:16:43,720
+ال osmotic pressure يعني عشان يكون isotonic تمام ب
+
+212
+00:16:43,720 --> 00:16:47,160
+provide isotonicity يعني عشان يخل المحلول isotonic
+
+213
+00:16:47,160 --> 00:16:51,580
+sodium sulfate طبعا وظيفة ال sodium sulfate أن هو
+
+214
+00:16:51,580 --> 00:16:56,380
+prevent roll off formation بيمنع ال roll off
+
+215
+00:16:56,380 --> 00:16:59,840
+formation تكون ال roll off formationmercuric
+
+216
+00:16:59,840 --> 00:17:03,320
+chloride اللي هو act as antiseptic اللي هو يعني زي
+
+217
+00:17:03,320 --> 00:17:08,720
+مادة متهرة او معاقمة تمنع نمو اي فطريات او عفن او
+
+218
+00:17:08,720 --> 00:17:12,860
+اي مايكروبات داخل هذا المحلول فبيضل نظيف بيضل خالي
+
+219
+00:17:12,860 --> 00:17:16,700
+من ال مايكروبات فمابيأثرش على القراءة انا وين ما
+
+220
+00:17:16,700 --> 00:17:22,480
+استخدمه بيضل نظيف ومايأثرلي على القراءةتمام؟ ذكرنا
+
+221
+00:17:22,480 --> 00:17:25,760
+احنا انه في محاضرة ال white pieces count انه لما
+
+222
+00:17:25,760 --> 00:17:30,040
+يكون في عندي في المحلول او على ال chamber او حتى
+
+223
+00:17:30,040 --> 00:17:34,000
+على العدسة في yeast خمائر او فطريات او عصن او اي
+
+224
+00:17:34,000 --> 00:17:38,930
+شيء ممكن يأثرلي على العد تمام؟فالmercuricucloride
+
+225
+00:17:38,930 --> 00:17:42,770
+act as antiseptic بيمنع نمو هذه الكائنات الدقيقة
+
+226
+00:17:42,770 --> 00:17:46,430
+اللي من شأنها تعيق عملية العد Distilled water أكيد
+
+227
+00:17:46,430 --> 00:17:50,770
+يعني المذيب في لهذه solutes كلها المذيب تبعها
+
+228
+00:17:50,770 --> 00:17:54,550
+distilled water تمام؟ إذا هذا هيام solution
+
+229
+00:17:54,550 --> 00:17:59,990
+isotonic بيمنع الرلوة وبرضه كمان antiseptic ويعني
+
+230
+00:18:01,520 --> 00:18:06,480
+سائل منيح لحتى نستخدمه ك diluent ل ال red blood
+
+231
+00:18:06,480 --> 00:18:10,300
+cell count أيضا في عندي gore solution موجود في
+
+232
+00:18:10,300 --> 00:18:13,760
+sodium sulfate صارنا بنعرف إيش يعني sodium sulfate
+
+233
+00:18:13,760 --> 00:18:16,780
+وضيفته ب prevention of low formation و ال glacial
+
+234
+00:18:16,780 --> 00:18:21,180
+acetic acid تمام و موجود أيضا distilled water
+
+235
+00:18:21,600 --> 00:18:25,620
+Citrate Formalin Solution النوع الرابع من المحاليل
+
+236
+00:18:25,620 --> 00:18:30,140
+مكوّن من الـ Trisodium Citrate و Formalin زي يعني
+
+237
+00:18:30,140 --> 00:18:34,860
+زي كأنه Buffer حتى يحافظ لي على الـRBCs تمام؟ أنتم
+
+238
+00:18:34,860 --> 00:18:38,540
+لاحظين هنا المكونات المحاليل مش محتاجين صبغلش لإن
+
+239
+00:18:38,540 --> 00:18:42,940
+الـRBCs أصلا يعني هي لونها Red فأنا لو جيت على ال
+
+240
+00:18:42,940 --> 00:18:46,220
+microscope شوفتها لو لعبت بالـFine Adjustment شوية
+
+241
+00:18:46,220 --> 00:18:49,080
+عشان أشوف ال details ممكن أشوف اللون الأحمر اللي
+
+242
+00:18:49,080 --> 00:18:53,220
+موجود داخل الخلايافسهل جدا أميزها لكن في ال white
+
+243
+00:18:53,220 --> 00:18:56,480
+pieces count كان ال solution لازم يكون فيه crystal
+
+244
+00:18:56,480 --> 00:19:00,160
+violet أو يعني صبغة معينة لحتى لما ألعب بال fine
+
+245
+00:19:00,160 --> 00:19:04,380
+adjustment أشوف أن ال white pieces مصبوغة عشان
+
+246
+00:19:04,380 --> 00:19:10,350
+أقدر أميزهاطيب بيحكيلي في بعض الحالات in certain
+
+247
+00:19:10,350 --> 00:19:15,150
+conditions Hayan solution may cause the clumping
+
+248
+00:19:15,150 --> 00:19:20,110
+of RBCs under law، تمام؟ و .. و .. و لكن يعني في
+
+249
+00:19:20,110 --> 00:19:24,550
+بعض الحالات ال Hayan solution ممكن يعملي clumping
+
+250
+00:19:24,550 --> 00:19:28,810
+لل RBCs، ممكن يعملي law formation، تجمعات لل RBCs،
+
+251
+00:19:28,810 --> 00:19:33,370
+فبالتالي إذا أنا صدفو أنا بستخدم Hayam و إجا عندي
+
+252
+00:19:33,370 --> 00:19:38,250
+patient و وجدت إنه عينته صار فيها clumping أو لورة
+
+253
+00:19:38,250 --> 00:19:41,250
+معناه هو هذا الشخص مش لازم أنا .. يعني هو ممكن
+
+254
+00:19:41,250 --> 00:19:46,250
+يكون حساس لمكونات Hayam solution فبالتالي مش لازم
+
+255
+00:19:46,250 --> 00:19:50,670
+أستخدم مع Hayam solution لازم أستخدمله للعد gore
+
+256
+00:19:50,670 --> 00:19:53,450
+solution تمام؟ Gore solution prevent these
+
+257
+00:19:53,450 --> 00:19:58,210
+problems بيمنع هذه المشاكلإذن في بعض الأحيان وليس
+
+258
+00:19:58,210 --> 00:20:04,030
+دوما تمام، gower solution يتفوق على هيام في منع ال
+
+259
+00:20:04,030 --> 00:20:07,470
+clumping و ال roll-off و هذا طبعا بيرجع لنوع
+
+260
+00:20:07,470 --> 00:20:12,240
+المريض يعني، ان دمه بيتأثر بمكونات هيام ولا لأ؟طيب
+
+261
+00:20:12,240 --> 00:20:16,120
+holy blood using edita or heparin as anticoagulant
+
+262
+00:20:16,120 --> 00:20:21,040
+طبعا انا عندى العينة تبعتي لازم يكون holy blood
+
+263
+00:20:21,040 --> 00:20:26,500
+بستخدم .. يكون طبعا ال tube edita او heparin طبعا
+
+264
+00:20:26,500 --> 00:20:30,670
+يكون فيها edita او heparinو ممكن كمان استخدم
+
+265
+00:20:30,670 --> 00:20:34,210
+capillary blood يعني من ال skin puncture باستخدام
+
+266
+00:20:34,210 --> 00:20:38,050
+الثومة بيبت يعني اعبي الثومة بيبت مباشرة طيب انا
+
+267
+00:20:38,050 --> 00:20:43,750
+ايش بلزمني؟ بلزمني بيبت تمام اما micro bibet او
+
+268
+00:20:43,750 --> 00:20:46,390
+اللي هي ال automatic bibet اللي انتوا بتشتغلوا
+
+269
+00:20:46,390 --> 00:20:50,350
+عليها في المختبرات او الثومة بيبت اللي مخصصة لل
+
+270
+00:20:50,350 --> 00:20:55,940
+RBCsتفتذكروا هاي الصورة اللي احنا ذكرنا .. عرضنا
+
+271
+00:20:55,940 --> 00:21:01,280
+فيها ثومة بيبت حكينا انه في عندي ثومة بيبت خاصة
+
+272
+00:21:01,280 --> 00:21:06,300
+بال white pieces count و ثومة بيبت خاصة بال R
+
+273
+00:21:06,300 --> 00:21:13,070
+pieces count تمام؟فإذا أنا بستخدم ال thoma pivot
+
+274
+00:21:13,070 --> 00:21:17,410
+اللي خاصة بال RBCs count حكينا حجمها أكبر شوية
+
+275
+00:21:17,410 --> 00:21:22,030
+لإنه نهاية التدريج عند 101 على خلاف ال YPCs نهاية
+
+276
+00:21:22,030 --> 00:21:25,790
+التدريج عند 11 طبعا أنا بملأها سواء كان من skin
+
+277
+00:21:25,790 --> 00:21:29,410
+puncture أو من ال tube اللي أنا عبتها يعني ال tube
+
+278
+00:21:29,410 --> 00:21:33,940
+فيها إيدي أو هبارين تمام؟ملقت فيها الدم اللي جمعته
+
+279
+00:21:33,940 --> 00:21:38,660
+من الوريد عملت mixing و بديت أعبي ال .. طبعا ال ..
+
+280
+00:21:38,660 --> 00:21:39,680
+ال .. ال .. ال .. ال .. ال .. ال .. ال .. ال .. ال
+
+281
+00:21:39,680 --> 00:21:39,800
+.. ال .. ال .. ال .. ال .. ال .. ال .. ال .. ال ..
+
+282
+00:21:39,800 --> 00:21:39,880
+ال .. ال .. ال .. ال .. ال .. ال .. ال .. ال .. ال
+
+283
+00:21:39,880 --> 00:21:40,580
+.. ال .. ال .. ال .. ال .. ال .. ال .. ال .. ال ..
+
+284
+00:21:40,580 --> 00:21:41,500
+ال .. ال .. ال .. ال .. ال .. ال .. ال .. ال .. ال
+
+285
+00:21:41,500 --> 00:21:41,840
+.. ال .. ال .. ال .. ال .. ال .. ال .. ال .. ال ..
+
+286
+00:21:41,840 --> 00:21:42,040
+ال .. ال .. ال .. ال .. ال .. ال .. ال .. ال .. ال
+
+287
+00:21:42,040 --> 00:21:42,480
+.. ال .. ال .. ال .. ال .. ال .. ال .. ال .. ال ..
+
+288
+00:21:42,480 --> 00:21:42,560
+ال .. ال .. ال .. ال .. ال .. ال .. ال .. ال .. ال
+
+289
+00:21:42,560 --> 00:21:44,940
+.. ال .. ال .. ال .. ال .. ال .. ال .. ال .. ال ..
+
+290
+00:21:44,940 --> 00:21:50,760
+.. التمام مع أنها سميكة من برا شوي لكن لأ المسار
+
+291
+00:21:50,760 --> 00:21:54,160
+دقيق جدا و الدم بيطلع بخاصية ال capillary action
+
+292
+00:21:54,160 --> 00:21:58,800
+تمام بملأ الدم لعند النص و بعد هيك بمسح ال
+
+293
+00:21:58,800 --> 00:22:01,800
+capillary بنظف ال excess blood اللي عليها من برا
+
+294
+00:22:01,800 --> 00:22:05,600
+لإنه هيأثرلي على ال dilution إذا دل و بعد هيك إيش
+
+295
+00:22:05,600 --> 00:22:11,600
+بعمل بروح بملأ لحد ال 101 بال diluent طبعا ال
+
+296
+00:22:11,600 --> 00:22:14,560
+diluent اللي إحنا راح نستخدمه في المعمل هو ال
+
+297
+00:22:14,560 --> 00:22:22,990
+saline هوأول محلول ذكرناه ك daily one هو
+
+298
+00:22:22,990 --> 00:22:25,670
+ال isotonic saline اللي هو المحلول الملحي الطبيعي
+
+299
+00:22:25,670 --> 00:22:28,750
+يعني اللي .. اللي احنا ذكرناه متوفر وموجود في
+
+300
+00:22:28,750 --> 00:22:32,430
+الصيدليات بيتعقم نستخدمه كتير لل seal suspension
+
+301
+00:22:32,430 --> 00:22:38,170
+لحتى يعني أستخدم .. أعمل معلق خلايا فيه يعني سهل
+
+302
+00:22:38,170 --> 00:22:41,370
+ومتوفر سهل تحضيره أيضا
+
+303
+00:22:45,910 --> 00:22:46,410
+تمام
+
+304
+00:22:50,990 --> 00:22:55,710
+إذن أنا محتاجة bybit سواء كانت thermobibet المخصصة
+
+305
+00:22:55,710 --> 00:22:59,570
+لل RBCs أو حتى automatic bybit محتاجة ال chamber
+
+306
+00:22:59,570 --> 00:23:04,990
+تمام اللي هو يعني ال slide المخصصة لعد الخلايا و
+
+307
+00:23:04,990 --> 00:23:08,750
+ال .. شوفوا معايا ال .. ال .. ال chamber بيكون
+
+308
+00:23:08,750 --> 00:23:12,870
+مقسم لال .. ال .. ال .. يعني لمناطق على عد ال
+
+309
+00:23:12,870 --> 00:23:17,400
+white pieces ومناطق لعد ال RBCsهلأ اذا أحيانا في
+
+310
+00:23:17,400 --> 00:23:21,740
+بعض ال chambers تمام؟ في بعض ال chambers ما بيكون
+
+311
+00:23:21,740 --> 00:23:25,120
+.. هذه المنطقة بتكون ممسوحة يعني مافيش فيها ..
+
+312
+00:23:25,120 --> 00:23:28,980
+يعني مش مخصص لعبد RBCs بس ل white BCs فبسميه new
+
+313
+00:23:28,980 --> 00:23:34,280
+power chamber تمام؟إذا كان ال chamber موجود فيه
+
+314
+00:23:34,280 --> 00:23:38,600
+مناطق لعد ال RBCs بالإضافة لعد ال YBCs بسميه
+
+315
+00:23:38,600 --> 00:23:42,960
+improved neopower chamber، طبعا؟ يعني محسن، يعني
+
+316
+00:23:42,960 --> 00:23:48,360
+chamber مضافة عليه منطقة عد ال .. ال RBCsطمام؟
+
+317
+00:23:48,360 --> 00:23:52,820
+محتاجة ال light microscope، محتاجة جوز، شاش أو
+
+318
+00:23:52,820 --> 00:23:57,680
+cotton أو قطعة يعني قماشية لتنظيف إما ال .. ال ..
+
+319
+00:23:57,680 --> 00:24:02,040
+ال chamber لتنظيف ال pipe من برا، ثم ال pipe
+
+320
+00:24:02,040 --> 00:24:06,500
+لتنظيف عدسات ال microscope، إحنا محتاجين جوزطيب
+
+321
+00:24:06,500 --> 00:24:09,620
+انا بدى اعمل dilution احنا حكينا انا بعمل dilution
+
+322
+00:24:09,620 --> 00:24:13,540
+في ال thermobibet باخد لنص بعدين ل 101 خلينا نحسب
+
+323
+00:24:13,540 --> 00:24:18,280
+ال total dilution لهذه التجربة
+
+324
+00:24:18,280 --> 00:24:24,080
+انا طبعا حكينا باخد نص blood بعدين بكمل ل 101
+
+325
+00:24:24,080 --> 00:24:33,710
+تمام؟طيب و ال .. فبالتالي حكينا إنه الدم دايما
+
+326
+00:24:33,710 --> 00:24:39,290
+بيرجع أو بيستقر ب .. ال .. المستودع هنا يعني هذه
+
+327
+00:24:39,290 --> 00:24:41,710
+المنطقة فقط بيكون فيها ال diligent في ال white
+
+328
+00:24:41,710 --> 00:24:44,670
+pieces بيكون فيها فقط tech في ال RPCs هيكون فيها
+
+329
+00:24:44,670 --> 00:24:50,830
+فقط saline تمام؟ طيبو لما اجي انا اعبي ال chamber
+
+330
+00:24:50,830 --> 00:24:53,770
+بتخلص من المحتوى ال stem اللي هو هذا الواحد اللي
+
+331
+00:24:53,770 --> 00:24:57,510
+هنا اللي هو عبارة عن diluent فقط لحاله فإذا انا
+
+332
+00:24:57,510 --> 00:25:02,410
+بتخلص من ال .. ال واحد عبارة عن تلت قطرات او اربع
+
+333
+00:25:02,410 --> 00:25:05,410
+قطرات بنزلهم على ال tissue فبصير ال dilution نص
+
+334
+00:25:05,410 --> 00:25:14,850
+لمية تمام؟ يعني واحد إلى متين sorry واحد إلى متين
+
+335
+00:25:15,880 --> 00:25:19,980
+تمام فهذه هي الفكرة هذا ال dilution اللي انا بحضره
+
+336
+00:25:19,980 --> 00:25:22,760
+يعني واحد إلى مية او واحد ل متين واحد يعني هذا ال
+
+337
+00:25:22,760 --> 00:25:28,420
+total dilution فممكن انا احضره باستخدام بايبت
+
+338
+00:25:28,420 --> 00:25:32,720
+يوتيوب غير ال thomabibet اه عادي يعني شوفوا انا
+
+339
+00:25:32,720 --> 00:25:38,820
+بدي احضر واحد ل .. ل dilution طبعا اللي هو متين و
+
+340
+00:25:38,820 --> 00:25:45,870
+ال total متين واحد طيب جدش بدي احط لو حطيت هنالو
+
+341
+00:25:45,870 --> 00:25:53,810
+حطيت من ال blood عشرين microliter تمام؟ هيكون في
+
+342
+00:25:53,810 --> 00:26:00,830
+ال .. ال .. هيكون في ال tube .. اه و هستخدم
+
+343
+00:26:00,830 --> 00:26:07,750
+diluent عبارة عن ال volume تبعه4000 اللي هو عشرين
+
+344
+00:26:07,750 --> 00:26:11,310
+في متين طبعا اللي هو الضعف إذا انا استخدمت .. إذا
+
+345
+00:26:11,310 --> 00:26:14,090
+الواحد كان عبارة عن عشرين المتين مرة من العشرين
+
+346
+00:26:14,090 --> 00:26:19,210
+كام؟ 4000 طبعا يعني أربعة أمقال فإذا ممكن أنا أحضر
+
+347
+00:26:19,210 --> 00:26:22,190
+في تيوب أحط عشرين microliter من ال blood و أربعة
+
+348
+00:26:22,190 --> 00:26:27,090
+أمقال من ال diluting fluid اللي هو ال saline طبعا
+
+349
+00:26:27,090 --> 00:26:29,290
+طيب
+
+350
+00:26:30,820 --> 00:26:35,060
+إذا ممكن أحضره إما بطريقة ثومابيبت أسحب للنص أكمل
+
+351
+00:26:35,060 --> 00:26:39,840
+ل 101 أتخلص من أعمل mixing أتخلص من أول 3-4 قطرات
+
+352
+00:26:39,840 --> 00:26:44,120
+إذا ال dilution 1 ل 2 ك dilution أولي طبعا أنا
+
+353
+00:26:44,120 --> 00:26:48,180
+مابحكيش عن ال total dilution أنا بحكي نسبة ال
+
+354
+00:26:48,180 --> 00:26:53,300
+blood إلى ال diluent لما بقول 1 ل 2 تمام؟ أو إما
+
+355
+00:26:53,300 --> 00:26:58,180
+باستخدام ال micro pipette العادية باستخدام تيوب
+
+356
+00:26:58,180 --> 00:27:03,930
+باخد diluent و بحط عليه ال bloodطيب، لاحظوا هنا
+
+357
+00:27:03,930 --> 00:27:08,090
+أنا بحط أربع أمقال من ال saline، تمام؟ و بحط عليها
+
+358
+00:27:08,090 --> 00:27:11,210
+عشرين microliter من ال blood عشرين microliter كمية
+
+359
+00:27:11,210 --> 00:27:17,030
+قليلة جدا، تمام؟ فأنا لما ب .. ب .. بحاول أفضّيها،
+
+360
+00:27:17,030 --> 00:27:21,030
+بفضّيها في داخل المحلول و بعمل mixing منيح لحتى
+
+361
+00:27:21,030 --> 00:27:24,710
+أتأكد أن الطب و أنا طالعة تكون نظيفة و بطلع ضغطة
+
+362
+00:27:24,710 --> 00:27:27,750
+علشان ما أاخد volume معايا و أنا طالعة ما أؤثر على
+
+363
+00:27:27,750 --> 00:27:31,250
+ال dilution، تمام؟نفس الخطوات اللى اتبعناها
+
+364
+00:27:31,250 --> 00:27:35,650
+ودكرناها وشددنا عليها فى فحص ال homoglobin لما كنا
+
+365
+00:27:35,650 --> 00:27:39,030
+ناخد خمسة أمقال من ال drapkin اضيف عليها عشرين
+
+366
+00:27:39,030 --> 00:27:42,610
+microliter من ال blood وحكيتلكم انه بنفوت لجوا
+
+367
+00:27:42,610 --> 00:27:46,690
+المحلول بفضل عشرين microliter و بعمل mixing منيح
+
+368
+00:27:46,690 --> 00:27:49,790
+عشان تطلع التد نظيفة و بطلع دفتر عساس ما اخد
+
+369
+00:27:49,790 --> 00:27:54,810
+volumeتمام طيب بملق ال chamber هعلمكم ان شاء الله
+
+370
+00:27:54,810 --> 00:27:59,190
+بالمختبر كيف احنا بنعبي ال chamber بحط عليه cover
+
+371
+00:27:59,190 --> 00:28:02,230
+slip و المسافة الفاصلة اللي هي ال 1 من 10 ملي متر
+
+372
+00:28:02,230 --> 00:28:06,750
+مكعب هادي بنملقها في المحلول اللي احنا حضرناه و
+
+373
+00:28:06,750 --> 00:28:10,490
+بحط هذا ال hematocytometer على ال microscope و
+
+374
+00:28:10,490 --> 00:28:13,730
+ببدأ اجيب ال field طبعا انا بجيب ال field على
+
+375
+00:28:13,730 --> 00:28:17,440
+العدسة عشرةتمام؟ في ال white pieces بجيبه على
+
+376
+00:28:17,440 --> 00:28:21,220
+أربعة و بعدين بعيد على أشرة تمام؟ لكن هنا بجيبه
+
+377
+00:28:21,220 --> 00:28:24,920
+على عشرة و بعيد على أربعين طيب فبجيب ال field على
+
+378
+00:28:24,920 --> 00:28:29,840
+عشرة إيش بدي أشوف؟ خلونا مع بعض نكبر ال slide هنا
+
+379
+00:28:29,840 --> 00:28:35,720
+هاي أنا طبعا بدي أركز أخلي اللي تحت العدسة هو
+
+380
+00:28:35,720 --> 00:28:40,070
+المربع المركزي هذا اللي هو تقاطع المضغوطتمام؟ بحط
+
+381
+00:28:40,070 --> 00:28:44,770
+على تقاطع المضغوط تحت العدسة طيب التقاطع المضغوط
+
+382
+00:28:44,770 --> 00:28:47,870
+هذا أول ما أجيبه طبعا هو عبارة عن إيش؟ هو عبارة عن
+
+383
+00:28:47,870 --> 00:28:52,650
+خمسة وعشرين مربع صغير، خمسة في خمسة، هاي واحد،
+
+384
+00:28:52,650 --> 00:28:57,430
+اتنين، تلاتة، أربعة، خمسةواحد اتنين تلاتة اربعة
+
+385
+00:28:57,430 --> 00:29:03,430
+خمسة خمسة خمسة خمسة وعشرين تمام؟ طيب ايش المربعات
+
+386
+00:29:03,430 --> 00:29:06,370
+اللي انا ب .. طبعا كل مربع من هدول الخمسة وعشرين
+
+387
+00:29:06,370 --> 00:29:11,970
+مقسم إلى ستاشر مربع يعني مقسم إلى ستاشر زي هنا
+
+388
+00:29:11,970 --> 00:29:16,050
+طيب؟ فعادي يعني لما انا اكبره على اربعين بيكبر
+
+389
+00:29:16,050 --> 00:29:19,250
+بيصير زي هيك بيصير عبارة عن ستاشر مربع كبار في بعض
+
+390
+00:29:19,250 --> 00:29:25,190
+طيب؟فالمربعات اللي أنا بستخدمها علشان أعد عليها هي
+
+391
+00:29:25,190 --> 00:29:29,230
+الأربع اللي على الأطراف و اللي في المركز تمام؟ إذا
+
+392
+00:29:29,230 --> 00:29:35,790
+أول إشي بحط ال chamber على العدسة
+
+393
+00:29:35,790 --> 00:29:41,040
+عشرة بجيب ال field تمام؟بجيب ال field بحيث انه
+
+394
+00:29:41,040 --> 00:29:44,240
+تكون منطق التقاطع المضغوط هي اللي تحت ال
+
+395
+00:29:44,240 --> 00:29:48,520
+microscope او تحت العدسة و بعدين بركب .. ب .. بكبر
+
+396
+00:29:48,520 --> 00:29:53,020
+طبعا على العدسة 40 فبي .. ب .. بخلي ان العدسة
+
+397
+00:29:53,020 --> 00:29:56,780
+تتوجه على احد المربعات هدول هذا المربع الكبير اللي
+
+398
+00:29:56,780 --> 00:30:00,860
+هو تقاطع المضغوط هو عبارة عن 25 مربع كل واحد من ال
+
+399
+00:30:00,860 --> 00:30:07,890
+25 مقسم إلى 16 مربع صغار يعني زي هدولتمام؟ طيب ف
+
+400
+00:30:07,890 --> 00:30:12,230
+.. ب .. ببدأ أعد فيهم طبعا ب .. ببدأ أعد كيف أعد
+
+401
+00:30:12,230 --> 00:30:16,510
+.. كيف بعرف حالي إن أنا في المربع الأول أو التاني
+
+402
+00:30:16,510 --> 00:30:19,730
+أو التالت أو كذا شوفوا معايا ب .. من خلال
+
+403
+00:30:19,730 --> 00:30:23,150
+المستطيلات هذه اللي على الجانب تمام؟ ب .. شوف ..
+
+404
+00:30:23,150 --> 00:30:26,130
+إذا كان في مستطيلات هنا على الجانب و هنا معناها
+
+405
+00:30:26,130 --> 00:30:30,310
+أنا في الأول إذا كانت المستطيلات هيك و هيك معناها
+
+406
+00:30:30,310 --> 00:30:35,340
+أنا في اللي .. اللي على الطرف التاني رقم اتنينهنا
+
+407
+00:30:35,340 --> 00:30:39,960
+رقم تلاتة تمام المستطيلات بهذا الشكل هنا أربعة و
+
+408
+00:30:39,960 --> 00:30:43,660
+هذا اللي في المركز بيكون طبعا كل مربع له حدود تلت
+
+409
+00:30:43,660 --> 00:30:47,260
+خطوط هك و تلت خطوط هك تمام هم مش مباينين بيكونوا
+
+410
+00:30:47,260 --> 00:30:51,660
+في ال .. في الصورة هنا لكن على ال .. ال microscope
+
+411
+00:30:51,660 --> 00:30:54,580
+هتشوفوهم فانت طبعا كيف بديك تمشي لما تصل إلى
+
+412
+00:30:54,580 --> 00:30:59,000
+المركز اما بمشي تلت خطوات لتحت يعني هي واحد اتنين
+
+413
+00:30:59,000 --> 00:31:04,040
+تلاتة واحد اتنين تلاتة اذا انا في المركزتمام؟ هيك
+
+414
+00:31:04,040 --> 00:31:08,000
+بيكون ال .. او يعني انه انت في المربع اترك اتنين
+
+415
+00:31:08,000 --> 00:31:12,700
+بعدين كمان اترك امشي اتنين بتلاقي حالك في المركز
+
+416
+00:31:12,700 --> 00:31:17,780
+هنتعلم هذه كلها خطوة بخطوة كيف احنا بنتحرك تمام؟
+
+417
+00:31:17,780 --> 00:31:21,480
+طيب بالزمني اعرف انه هذا المربع الكبير اللي بيحتوي
+
+418
+00:31:21,480 --> 00:31:25,400
+على خمسة و عشرين مربع صغير هو نفس أبعد هذا يعني
+
+419
+00:31:25,400 --> 00:31:31,060
+هذا واحدو هذا واحد millimeter تمام طب هذا الصغير
+
+420
+00:31:31,060 --> 00:31:35,880
+طب ما هو إذا هذا واحد و مقسم إلى خمس أجزاء تمام
+
+421
+00:31:35,880 --> 00:31:40,340
+خمس مربعات معناها البعد المربع الصغير اللي هو
+
+422
+00:31:40,340 --> 00:31:45,450
+عبارة عن اتنين من عشرة اتنين من عشرة تماممهمين
+
+423
+00:31:45,450 --> 00:31:51,350
+للحسابات، لذا هاي هنا تقاطع المضغوط، بعدين بجيبه
+
+424
+00:31:51,350 --> 00:31:55,210
+على العدسة عشرة، بحول على أربعين، بحدد أي مربع أنا
+
+425
+00:31:55,210 --> 00:31:58,710
+فيه، ببدأ أعد، بعدين بنتقل إلى المربعات الأخرى، و
+
+426
+00:31:58,710 --> 00:32:03,450
+بعدها، تمام؟ و عارفنا كيف بحدد حالي وين أنا في أي
+
+427
+00:32:03,450 --> 00:32:07,890
+مربع، بعد ما أخلص عد، بدي أجي أعمل الحساباتطبعا ال
+
+428
+00:32:07,890 --> 00:32:12,210
+total RPCs count تبعي نفس قانون ال total YPCs
+
+429
+00:32:12,210 --> 00:32:16,110
+count اللي هو عبارة عن N ولكن هنا N هو مجموع
+
+430
+00:32:16,110 --> 00:32:20,270
+الخلايا في خمس مربعات، تمام؟ يعني إذا عديت في
+
+431
+00:32:20,270 --> 00:32:26,870
+منطقة واحدة فقط بجمع الخمس مربعات و بعود في N إذا
+
+432
+00:32:26,870 --> 00:32:30,110
+عديت في المنطقة تانية من الات shape لازم أقسم ع
+
+433
+00:32:30,110 --> 00:32:33,250
+اتنين لإن بكون عديت عشر مربعات، خمسة فوق وخمسة تحت
+
+434
+00:32:33,680 --> 00:32:38,280
+تمام؟ فبقى أقسم على اتنين ضرب ال dilution factor
+
+435
+00:32:38,280 --> 00:32:43,760
+طبعا احنا حكينا ان ال dilution واحد إلى متين تمام؟
+
+436
+00:32:43,760 --> 00:32:49,880
+فال .. ال .. ال dilution factor متين تمام؟ انا
+
+437
+00:32:49,880 --> 00:32:54,900
+بفضل يا بنات ان هذه ال .. ال .. المعلومة بس
+
+438
+00:32:54,900 --> 00:32:59,120
+اتعدلوها و اتخلوها .. اتخلوها متين هيك لإنه طبعا
+
+439
+00:32:59,120 --> 00:33:03,790
+هي .. هي الأفضل هي الأدق هيك لتكون متينطيب ف .. ك
+
+440
+00:33:03,790 --> 00:33:07,990
+.. ك total يعني لإنه أنا باخد الحجم نص و بكمل لل
+
+441
+00:33:07,990 --> 00:33:12,190
+.. للمية و واحد يعني النص أصلا عبارة .. يعني لما
+
+442
+00:33:12,190 --> 00:33:17,390
+أجيب .. لما أفضي واحد كمان النص و ال .. ال .. ال
+
+443
+00:33:17,390 --> 00:33:20,810
+.. المية ما هو النص محتل جزء من المية تمام؟ يعني
+
+444
+00:33:20,810 --> 00:33:25,050
+ال total هي المية تمام؟ فبده .. لما بدي أحاول
+
+445
+00:33:25,050 --> 00:33:27,910
+أتخلص من النص هذا يعني أخليه واحد بده يصير هذه
+
+446
+00:33:27,910 --> 00:33:28,390
+متين
+
+447
+00:33:33,940 --> 00:33:38,400
+تمام في ال dilution factor اللي هو 200 و ال volume
+
+448
+00:33:38,400 --> 00:33:42,160
+correction factor طب احنا بنعد per microliter واحد
+
+449
+00:33:42,160 --> 00:33:46,100
+طب انا هلأ عديت الحجم لازم يكون microliter واحد
+
+450
+00:33:46,100 --> 00:33:49,600
+لإن ال .. ال .. الوحدة اللي بدي استخدمها هي عبارة
+
+451
+00:33:49,600 --> 00:33:58,020
+عن سل per microliter تمام؟ سل per
+
+452
+00:33:58,020 --> 00:34:05,250
+microliter تمام؟طيب ف .. بدي بال microliter الواحد
+
+453
+00:34:05,250 --> 00:34:08,630
+كام بيكون خلايا فبدأ أعمل بحاجة اسمها volume
+
+454
+00:34:08,630 --> 00:34:12,050
+correction و أجيبكوا volume correction factor طب
+
+455
+00:34:12,050 --> 00:34:15,010
+نحسب الحجم نفس ما كنا نعمل في ال white pieces بدي
+
+456
+00:34:15,010 --> 00:34:19,090
+أحسب الحجم اللي انعدت فيه الخلايا احنا حكينا ان
+
+457
+00:34:19,090 --> 00:34:24,440
+المربع هذا كل هذانفس الكبير هذا هذا حكينا في ال
+
+458
+00:34:24,440 --> 00:34:26,840
+white pieces كام حجم وكنا نعد فيه white pieces هذا
+
+459
+00:34:26,840 --> 00:34:32,460
+واحد في واحد تمام؟ أبعاده فهذا بيكون واحد في واحد
+
+460
+00:34:32,460 --> 00:34:36,940
+زيه طب أنا بعد في هذا الصغير اللي هو عبارة عن خمس
+
+461
+00:34:36,940 --> 00:34:42,220
+المسافة أبعاده صح؟ هي واحد اتنين تلاتة أربعة خمسة
+
+462
+00:34:42,220 --> 00:34:46,260
+إذا هذا البعد هو عبارة عن خمس المسافة كلها إذا
+
+463
+00:34:46,260 --> 00:34:50,670
+المسافة كلها واحد خمسها كم رح يكون؟الخمس يعني
+
+464
+00:34:50,670 --> 00:34:55,230
+اتنين من عشرة .. اتنين من عشرة في اتنين من عشرة
+
+465
+00:34:55,230 --> 00:34:59,950
+تمام في ال point واحد اللي هو المسافة ما بين ال
+
+466
+00:34:59,950 --> 00:35:04,870
+chamber نفسه و ال cover slip اللي هي الارتفاع تبع
+
+467
+00:35:04,870 --> 00:35:07,910
+العينة او الارتفاع اللي موجود .. اللي موجودة فيه
+
+468
+00:35:07,910 --> 00:35:10,490
+العينة اللي هو المسافة ما بين ال chamber و ال
+
+469
+00:35:10,490 --> 00:35:14,900
+cover slip واحد من عشرة حكينا .. ذكرناهاتمام؟ فإذا
+
+470
+00:35:14,900 --> 00:35:19,640
+بدي يكون الحجم فاصلة اتنين ضرب فاصلة اتنين ضرب
+
+471
+00:35:19,640 --> 00:35:24,960
+فاصلة واحد طول عرض ارتفاع فبدي يكون هذا هو الحجم
+
+472
+00:35:24,960 --> 00:35:28,200
+تمام؟ أنا بعيد في خمس مربعات إذا الحجم الكل اللي
+
+473
+00:35:28,200 --> 00:35:33,200
+عديت فيه خمسة في هذا فهيطلع ليإتنين من مية تمام؟
+
+474
+00:35:33,200 --> 00:35:36,700
+إتنين من مية لما أعمل .. أجيب factor يعني مقلوبها
+
+475
+00:35:36,700 --> 00:35:41,180
+اللي هو عبارة عن خمسين فإذا اللي هو عبارة عن ال ..
+
+476
+00:35:41,180 --> 00:35:46,960
+ال .. القانون ال N عدد المجموع الخلايا في خمس
+
+477
+00:35:46,960 --> 00:35:55,020
+مربعات ضرب متين ضرب خمسين يعني N في عشر تلاف بطل
+
+478
+00:35:55,020 --> 00:35:59,740
+عدد ال Rbcsطبعا in certain conditions such as
+
+479
+00:35:59,740 --> 00:36:02,540
+polycythemia, red blood cell count may be
+
+480
+00:36:02,540 --> 00:36:05,500
+extremely high في بعض الحالات زي ال polycythemia
+
+481
+00:36:05,500 --> 00:36:09,980
+ال RBCs بتكون عالية جدا، إيش بدي أعمل؟ والله هذه
+
+482
+00:36:09,980 --> 00:36:13,500
+المعلومة ذكرنا إشي مشابه لإلها في ال YBCs count
+
+483
+00:36:13,620 --> 00:36:16,020
+حكينا في ال white pieces count لما يكون عندي
+
+484
+00:36:16,020 --> 00:36:20,620
+leukocytosis يعني ارتفاع في ال white pieces بروح
+
+485
+00:36:20,620 --> 00:36:25,260
+بزود ال dilution تمام؟ و هنا أيضا كمان بدي ازود
+
+486
+00:36:25,260 --> 00:36:28,780
+نفس الاشي بدي ازود ال dilution ففي حالات ال
+
+487
+00:36:28,780 --> 00:36:32,000
+polycythemia اللي فيها ارتفاع في ال red blood cell
+
+488
+00:36:32,000 --> 00:36:36,780
+بحكيلي انه زودي ال dilution يعني ده اخففي اكتر
+
+489
+00:36:36,780 --> 00:36:40,320
+اعملي larger dilution يعني بدل ما اعمل 1 ل 200
+
+490
+00:36:40,320 --> 00:36:46,260
+خليني اعمل 1 ل 300 تمام؟خليني أعمل واحد لتلت مية
+
+491
+00:36:46,260 --> 00:36:51,060
+يعني بدي أضيف عشرين microliter لست أمقال ال blood
+
+492
+00:36:51,060 --> 00:36:55,960
+طيب و الشخص
+
+493
+00:36:55,960 --> 00:37:03,140
+اللي عنده .. الشخص اللي عنده anemia تمامالشخص اللي
+
+494
+00:37:03,140 --> 00:37:08,220
+عنده anemia يعني low RBC count بقلل ال dilution
+
+495
+00:37:08,220 --> 00:37:11,600
+بعمل smaller dilution يعني بدل ما هو 1 ل 200 خلينا
+
+496
+00:37:11,600 --> 00:37:17,690
+نعمل 1 ل 100 يعني 20 microliter على 2 ml في tubeمش
+
+497
+00:37:17,690 --> 00:37:22,370
+في الصومة بايبت make certain تكوني متأكدة انه ال
+
+498
+00:37:22,370 --> 00:37:25,610
+بايبت ال hematocytometer and the covering glass
+
+499
+00:37:25,610 --> 00:37:29,350
+are free from their cleaned and dried blood لازم
+
+500
+00:37:29,350 --> 00:37:32,510
+الأدوات اللي بنستخدمها سواء كانت بايبت او
+
+501
+00:37:32,510 --> 00:37:35,250
+hematocytometer او حتى ال cover slip ال covering
+
+502
+00:37:35,250 --> 00:37:41,870
+glassلازم يكون خالي من الغبرة، أي أشياء بتعمل
+
+503
+00:37:41,870 --> 00:37:46,830
+اتساخ، بتلتسق عليه، بتعمل يعني إعاقة للرؤية و
+
+504
+00:37:46,830 --> 00:37:50,970
+للعدد و أيضا الدم الجاف ensure that the diluting
+
+505
+00:37:50,970 --> 00:37:54,670
+fluid is free from blood، كمان ال diluting fluid
+
+506
+00:37:54,670 --> 00:37:58,190
+اللي أنا بدى أستخدمه لازم يكون نظيف، مافيش فيه دم
+
+507
+00:37:58,190 --> 00:38:03,250
+تمام أو أي contamination أخرىو ال RPCs takes
+
+508
+00:38:03,250 --> 00:38:07,910
+longer to perform than YPCs طبعا عد ال RPCs بيكون
+
+509
+00:38:07,910 --> 00:38:11,910
+بياخد وقت أطول لإن ان انت بتعدي في خمس مربعات و
+
+510
+00:38:11,910 --> 00:38:15,250
+بديك فترة لما تنتقل من مربع لآخر و أصلا تجيب
+
+511
+00:38:15,250 --> 00:38:19,410
+المربع الأساسي اللي هو تقاطع المضغوط فيعني بديك
+
+512
+00:38:19,410 --> 00:38:24,930
+فترة ل .. تتمرني عليه فهو بياخد وقت أطول ال RPCs
+
+513
+00:38:24,930 --> 00:38:29,840
+countفي .. ك .. كعمل يعني كمدة عمل فبالتالي بيحكي
+
+514
+00:38:29,840 --> 00:38:31,320
+لي إنه لازم
+
+515
+00:38:34,000 --> 00:38:40,040
+لازم انت تتأكدي انه ال .. ال .. ال chamber ما جاف
+
+516
+00:38:40,040 --> 00:38:45,040
+تمام؟ لإنه جفاف ال chamber هي .. هيأثرلي على العدل
+
+517
+00:38:45,040 --> 00:38:48,020
+لازم انا ساعتها اشتغل proceed as quickly as
+
+518
+00:38:48,020 --> 00:38:51,840
+possible تمام؟ و ايضا احد العوامل اللي بيخلي انه
+
+519
+00:38:51,840 --> 00:38:56,060
+عملية ال RBCs count تاخد وقت أطول انه اصلا انت
+
+520
+00:38:56,060 --> 00:39:00,880
+بتعدي عدد خلايا اكبريعني هتشوفوا ال RPC ال white
+
+521
+00:39:00,880 --> 00:39:05,000
+PCs يعني يلّا .. يلّا يلّا تشوفي خلايا في المربعات
+
+522
+00:39:05,000 --> 00:39:08,080
+لكن في ال RPC بتشوفي عدد كبير من ال .. من الخلايا
+
+523
+00:39:08,080 --> 00:39:13,040
+و لازم تعديها فلإن عددها أكبر بتاخد وقت أطول أيضًا
+
+524
+00:39:13,040 --> 00:39:16,140
+في العد the range of error نسبة الخطأ في هذه
+
+525
+00:39:16,140 --> 00:39:18,660
+الطريقة في هذه التجربة مقارنتها بال .. بال
+
+526
+00:39:18,660 --> 00:39:24,500
+automatic يعني بال .. بالأجهزة من 10 إلى 20% نسبة
+
+527
+00:39:24,500 --> 00:39:29,360
+خطأهيك بنكون انتهينا من محاضرتنا لليوم طبعا بس بدي
+
+528
+00:39:29,360 --> 00:39:34,240
+أذكرلكم معلومة احنا ما ذكرناها بال white pieces
+
+529
+00:39:34,240 --> 00:39:36,120
+count حكينا انه
+
+530
+00:39:39,360 --> 00:39:43,980
+هنا ال .. ال R .. هذا ال normal range ل ال .. ال
+
+531
+00:39:43,980 --> 00:39:46,940
+.. ال RPCs count وحكينا عددها بالملايين عشر أقل
+
+532
+00:39:46,940 --> 00:39:50,080
+ستة سل بين microliter الوحدة سل بين microliter
+
+533
+00:39:50,080 --> 00:39:56,160
+تمام ال white PCs عددها بالألف تمام و هي من أربعة
+
+534
+00:39:56,160 --> 00:40:01,360
+و نص ألف لحداشر ألفsilver microliter طبعا لما بدي
+
+535
+00:40:01,360 --> 00:40:06,320
+احول ل liter بدي اضرب في عشرة او استسعى بس لو per
+
+536
+00:40:06,320 --> 00:40:17,360
+microliter بدها تكون زي هيك من اربعة و نص بدها
+
+537
+00:40:17,360 --> 00:40:24,360
+تكون هيك تمام ضرب عشرة
+
+538
+00:40:26,020 --> 00:40:30,520
+هي العشرة أس تلاتة
+
+539
+00:40:30,520 --> 00:40:38,900
+تمام هدي أس تلاتة مافيش عندي طريقة أرفعها مش مهم
+
+540
+00:40:38,900 --> 00:40:42,020
+هي هدي أس يعني power تمام
+
+541
+00:40:49,340 --> 00:40:53,100
+تمام انتوا افهموها انها هي هدي أصبور عشرة أص تلاتة
+
+542
+00:40:53,100 --> 00:41:00,640
+طبعا ايش ال .. هي .. هي كبير microliter تمام؟ هاي
+
+543
+00:41:00,640 --> 00:41:04,680
+ال microliter طبعا يعني انا ب .. بكتبها تجاوزا أم
+
+544
+00:41:04,680 --> 00:41:06,700
+ال .. sorry
+
+545
+00:41:09,010 --> 00:41:14,010
+ب .. بكتوبها تجاوزا هي اللي زي ال .. ال capital M
+
+546
+00:41:14,010 --> 00:41:18,470
+و ال L لكن هي يعني microliter بتنكتب بشكل .. بشكل
+
+547
+00:41:18,470 --> 00:41:23,310
+مختلف اللي هي هيك، تمام؟ أو ممكن أحكي زي .. يعني
+
+548
+00:41:23,310 --> 00:41:28,690
+أخد و أحكي سلper microliter يعني نفس ال .. الوحدة
+
+549
+00:41:28,690 --> 00:41:32,730
+يعني سواء كتبت العدد و قولت per microliter او حكيت
+
+550
+00:41:32,730 --> 00:41:36,210
+silver microliter نفس المعنى تمام؟ اذا هذا هو ال
+
+551
+00:41:36,210 --> 00:41:40,430
+range من اربعة و نص لحداشر الف او من اي يعني اربعة
+
+552
+00:41:40,430 --> 00:41:43,170
+تلاف و نص لحداشر الف و في ناس بتحكي من تلت تلاف
+
+553
+00:41:43,170 --> 00:41:48,030
+لحداشر الفطبعا و Jadir بتذكر أن هذه ال normal
+
+554
+00:41:48,030 --> 00:41:52,750
+ranges بتختلف طبعا من فئة إلى أخرى من ناس من
+
+555
+00:41:52,750 --> 00:41:56,650
+population ل population من مختبر ل مختبر فبالتالي
+
+556
+00:41:56,650 --> 00:41:59,610
+احنا مابنحفظ normal ranges ولكن على الأقل بنكون
+
+557
+00:41:59,610 --> 00:42:04,280
+يعني ..مدركينها يعني okay طبعا أنا مش .. مش طالبة
+
+558
+00:42:04,280 --> 00:42:07,200
+منك تحفظي بال .. بالضبط بالضبط ال normal ranges بس
+
+559
+00:42:07,200 --> 00:42:11,340
+على الأقل تكوني عارفة يعني أشياء في .. بنسميها
+
+560
+00:42:11,340 --> 00:42:14,880
+headlines يعني أنه مثلا والله ال homoglobin عند ال
+
+561
+00:42:14,880 --> 00:42:20,160
+female من ال 11 ل 13 أو من 11 ل 14 من 3000 ل 11000
+
+562
+00:42:20,160 --> 00:42:25,560
+أو من 4000 ل .. ل 11000 ال YPCs ما بين الأربعة ل
+
+563
+00:42:25,560 --> 00:42:30,820
+.. ل الخمسة و نص ال .. ال .. ال Y .. ال RPCs وها
+
+564
+00:42:30,820 --> 00:42:31,120
+كذا
+
+565
+00:42:34,850 --> 00:42:39,770
+تمام؟ هيك بنكون وصلنا لمحاضرتنا .. لنهاية محاضرتنا
+
+566
+00:42:39,770 --> 00:42:43,690
+لليوم أتمنى إنها تكون بسيطة عليكم يعطيكم ألف عافية
+
diff --git a/PL9fwy3NUQKwY9jl8xw-bWkVZkWcGGyspd/FJ1Ngmal9to_raw.json b/PL9fwy3NUQKwY9jl8xw-bWkVZkWcGGyspd/FJ1Ngmal9to_raw.json
new file mode 100644
index 0000000000000000000000000000000000000000..fa602f6c4e17597ea390cbb20ca2270ec0b2f2f8
--- /dev/null
+++ b/PL9fwy3NUQKwY9jl8xw-bWkVZkWcGGyspd/FJ1Ngmal9to_raw.json
@@ -0,0 +1 @@
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حجم من الدم و باخد حجم من ال diligent طيب خصائص ال diligent كان في ال white pieces count انه لازم يكسرلي ال .. ال RPCs و يحفظلي ال .. ال white pieces و يصبغها علشان أشوفها تمام؟ لكن هنا أنا صح بعمل dilution باخد حجم من ال blood تمام؟ و حجم من ال diligent ولكن", "tokens": [39237, 10943, 22807, 4032, 19452, 2655, 4724, 47283, 3215, 11331, 7435, 2304, 9154, 32748, 2304, 4032, 4724, 47283, 3215, 11331, 7435, 2304, 9154, 2423, 50251, 23032, 1829, 3555, 16490, 9381, 16373, 9381, 2423, 50251, 25961, 8978, 2423, 2418, 3755, 1207, 16472, 3224, 5296, 31377, 2304, 7251, 4117, 3794, 2288, 20292, 2423, 4386, 2423, 497, 12986, 82, 4032, 7251, 5016, 5172, 19913, 20292, 2423, 4386, 2423, 2418, 3755, 4032, 7251, 9381, 3555, 17082, 11296, 11203, 8592, 7649, 5551, 8592, 38688, 11296, 46811, 10943, 22807, 44381, 34105, 41850, 20328, 5016, 4724, 25957, 1211, 11504, 1448, 4724, 47283, 3215, 11331, 7435, 2304, 9154, 2423, 3390, 46811, 10943, 22807, 4032, 11331, 7435, 2304, 9154, 2423, 50251, 35525, 19452], 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"لازم يكون ال diligent isotonic fluid وطبعا احنا في محاضرة من المحاضرات فرقنا اش يعني hypotonic؟ اش يعني hypertonic؟ واش يعني isotonic؟ وحكينا ان ال isotonic هو اللي بيحافظ على شكل الخلايا مابيخليهاش تتكسر يصير لها lysis او swelling او حتى كمان مابيصيرلهاش shrinkage", "tokens": [1211, 31377, 2304, 7251, 30544, 2423, 50251, 38018, 11630, 9113, 4032, 9566, 3555, 3615, 995, 1975, 5016, 8315, 8978, 3714, 5016, 46958, 25720, 9154, 9673, 5016, 46958, 2288, 9307, 6156, 2288, 4587, 8315, 1975, 8592, 37495, 22653, 7420, 310, 11630, 22807, 1975, 8592, 37495, 22653, 9848, 1756, 299, 22807, 4032, 33599, 37495, 22653, 38018, 11630, 22807, 4032, 5016, 4117, 1829, 8315, 16472, 2423, 38018, 11630, 31439, 13672, 1829, 4724, 1829, 5016, 31845, 19913, 15844, 13412, 28820, 33962, 15040, 25528, 3714, 16758, 1829, 9778, 20292, 3224, 33599, 6055, 2655, 4117, 3794, 2288, 7251, 9381, 13546, 5296, 11296, 287, 4642, 1975, 2407, 33127, 1975, 2407, 11331, 49975, 9122, 2304, 7649, 3714, 16758, 1829, 9381, 13546, 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1010.24, "end": 1010.9, "word": " sulfate", "probability": 0.92919921875}, {"start": 1010.9, "end": 1011.34, "word": " أن", "probability": 0.47802734375}, {"start": 1011.34, "end": 1011.58, "word": " هو", "probability": 0.64501953125}, {"start": 1011.58, "end": 1012.16, "word": " prevent", "probability": 0.64013671875}, {"start": 1012.16, "end": 1012.48, "word": " roll", "probability": 0.505859375}, {"start": 1012.48, "end": 1012.7, "word": " off", "probability": 0.39501953125}, {"start": 1012.7, "end": 1013.24, "word": " formation", "probability": 0.970703125}, {"start": 1013.24, "end": 1014.38, "word": " بيمنع", "probability": 0.8306884765625}, {"start": 1014.38, "end": 1014.8, "word": " ال", "probability": 0.44580078125}, {"start": 1014.8, "end": 1016.22, "word": " roll", "probability": 0.8857421875}, {"start": 1016.22, "end": 1016.38, "word": " off", "probability": 0.7998046875}, {"start": 1016.38, "end": 1016.82, "word": " formation", "probability": 0.984375}, {"start": 1016.82, 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1163.49, "end": 1164.03, "word": " يعملي", "probability": 0.8567708333333334}, {"start": 1164.03, "end": 1164.55, "word": " clumping", "probability": 0.908935546875}, {"start": 1164.55, "end": 1164.79, "word": " لل", "probability": 0.86279296875}, {"start": 1164.79, "end": 1165.43, "word": " RBCs،", "probability": 0.82135009765625}, {"start": 1165.43, "end": 1165.61, "word": " ممكن", "probability": 0.985595703125}, {"start": 1165.61, "end": 1166.01, "word": " يعملي", "probability": 0.7980143229166666}, {"start": 1166.01, "end": 1166.27, "word": " law", "probability": 0.57861328125}, {"start": 1166.27, "end": 1166.85, "word": " formation،", "probability": 0.808837890625}, {"start": 1166.85, "end": 1167.61, "word": " تجمعات", "probability": 0.98037109375}, {"start": 1167.61, "end": 1167.77, "word": " لل", "probability": 0.96435546875}, {"start": 1167.77, "end": 1168.81, "word": " RBCs،", "probability": 0.8546142578125}, {"start": 1168.81, "end": 1169.49, "word": " فبالتالي", "probability": 0.98193359375}, {"start": 1169.49, "end": 1170.01, "word": " إذا", "probability": 0.877685546875}, {"start": 1170.01, "end": 1170.25, "word": " أنا", "probability": 0.89990234375}, {"start": 1170.25, "end": 1170.75, "word": " صدف", "probability": 0.9391276041666666}], "temperature": 1.0}, {"id": 53, "seek": 119558, "start": 1171.11, "end": 1195.59, "text": "و أنا بستخدم Hayam و إجا عندي patient و وجدت إنه عينته صار فيها clumping أو لورة معناه هو هذا الشخص مش لازم أنا .. يعني هو ممكن يكون حساس لمكونات Hayam solution فبالتالي مش لازم أستخدم مع Hayam solution لازم أستخدمله للعد gore solution تمام؟ Gore solution prevent these problems بيمنع هذه المشاكل", "tokens": [2407, 41850, 4724, 14851, 9778, 40448, 8721, 335, 4032, 11933, 7435, 995, 18871, 16254, 4537, 4032, 49610, 3215, 2655, 36145, 3224, 6225, 9957, 47395, 20328, 9640, 8978, 11296, 596, 35313, 34051, 5296, 13063, 3660, 20449, 8315, 3224, 31439, 23758, 25124, 9778, 9381, 37893, 5296, 31377, 2304, 41850, 4386, 37495, 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"probability": 0.9763997395833334}, {"start": 1181.93, "end": 1182.73, "word": " لمكونات", "probability": 0.88779296875}, {"start": 1182.73, "end": 1183.05, "word": " Hayam", "probability": 0.84375}, {"start": 1183.05, "end": 1183.63, "word": " solution", "probability": 0.583984375}, {"start": 1183.63, "end": 1185.11, "word": " فبالتالي", "probability": 0.985107421875}, {"start": 1185.11, "end": 1185.95, "word": " مش", "probability": 0.966796875}, {"start": 1185.95, "end": 1186.25, "word": " لازم", "probability": 0.9957682291666666}, {"start": 1186.25, "end": 1186.79, "word": " أستخدم", "probability": 0.9339599609375}, {"start": 1186.79, "end": 1187.33, "word": " مع", "probability": 0.96044921875}, {"start": 1187.33, "end": 1187.81, "word": " Hayam", "probability": 0.7900390625}, {"start": 1187.81, "end": 1188.37, "word": " solution", "probability": 0.97021484375}, {"start": 1188.37, "end": 1189.07, "word": " لازم", "probability": 0.9371744791666666}, {"start": 1189.07, "end": 1189.75, "word": " أستخدمله", "probability": 0.8775390625}, {"start": 1189.75, "end": 1190.23, "word": " للعد", "probability": 0.6217447916666666}, {"start": 1190.23, "end": 1190.67, "word": " gore", "probability": 0.5806884765625}, {"start": 1190.67, "end": 1191.27, "word": " solution", "probability": 0.9755859375}, {"start": 1191.27, "end": 1191.91, "word": " تمام؟", "probability": 0.7140299479166666}, {"start": 1191.91, "end": 1192.13, "word": " Gore", "probability": 0.39501953125}, {"start": 1192.13, "end": 1192.73, "word": " solution", "probability": 0.96875}, {"start": 1192.73, "end": 1193.13, "word": " prevent", "probability": 0.69970703125}, {"start": 1193.13, "end": 1193.45, "word": " these", "probability": 0.787109375}, {"start": 1193.45, "end": 1194.07, "word": " problems", "probability": 0.86572265625}, {"start": 1194.07, "end": 1194.75, "word": " بيمنع", "probability": 0.8154296875}, {"start": 1194.75, "end": 1194.97, "word": " هذه", "probability": 0.6865234375}, {"start": 1194.97, "end": 1195.59, "word": " المشاكل", "probability": 0.992919921875}], "temperature": 1.0}, {"id": 54, "seek": 121115, "start": 1195.95, "end": 1211.15, "text": "إذن في بعض الأحيان وليس دوما تمام، gower solution يتفوق على هيام في منع ال clumping و ال roll-off و هذا طبعا بيرجع لنوع المريض يعني، ان دمه بيتأثر بمكونات هيام ولا لأ؟", "tokens": [28814, 8848, 1863, 8978, 45030, 11242, 16247, 5016, 1829, 7649, 4032, 20292, 3794, 11778, 2407, 15042, 46811, 10943, 12399, 352, 1554, 3827, 7251, 2655, 5172, 30543, 15844, 39896, 10943, 8978, 9154, 3615, 2423, 596, 35313, 4032, 2423, 3373, 12, 4506, 4032, 23758, 23032, 3555, 3615, 995, 4724, 13546, 7435, 3615, 5296, 1863, 45367, 9673, 16572, 11242, 37495, 22653, 12399, 16472, 11778, 2304, 3224, 4724, 36081, 10721, 49115, 4724, 2304, 4117, 2407, 8315, 2655, 39896, 10943, 49429, 5296, 10721, 22807], "avg_logprob": -0.2744140762835741, "compression_ratio": 1.4293193717277486, "no_speech_prob": 1.6689300537109375e-06, "words": [{"start": 1195.95, "end": 1196.27, "word": "إذن", "probability": 0.555908203125}, {"start": 1196.27, "end": 1196.43, "word": " في", "probability": 0.87744140625}, {"start": 1196.43, "end": 1196.89, "word": " بعض", "probability": 0.994140625}, {"start": 1196.89, "end": 1197.75, "word": " الأحيان", "probability": 0.9498291015625}, {"start": 1197.75, "end": 1198.21, "word": " وليس", "probability": 0.8736979166666666}, {"start": 1198.21, "end": 1198.89, "word": " دوما", "probability": 0.8253580729166666}, {"start": 1198.89, "end": 1200.03, "word": " تمام،", "probability": 0.6444498697916666}, {"start": 1200.03, "end": 1200.37, "word": " gower", "probability": 0.28668212890625}, {"start": 1200.37, "end": 1200.79, "word": " solution", "probability": 0.92529296875}, {"start": 1200.79, "end": 1201.45, "word": " يتفوق", "probability": 0.9603271484375}, {"start": 1201.45, "end": 1201.71, "word": " على", "probability": 0.93603515625}, {"start": 1201.71, "end": 1202.23, "word": " هيام", "probability": 0.636474609375}, 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1206.83, "end": 1207.47, "word": " لنوع", "probability": 0.9064127604166666}, {"start": 1207.47, "end": 1208.25, "word": " المريض", "probability": 0.9228515625}, {"start": 1208.25, "end": 1208.61, "word": " يعني،", "probability": 0.70263671875}, {"start": 1208.61, "end": 1208.69, "word": " ان", "probability": 0.31396484375}, {"start": 1208.69, "end": 1208.91, "word": " دمه", "probability": 0.9864908854166666}, {"start": 1208.91, "end": 1209.51, "word": " بيتأثر", "probability": 0.9765625}, {"start": 1209.51, "end": 1210.15, "word": " بمكونات", "probability": 0.8990071614583334}, {"start": 1210.15, "end": 1210.49, "word": " هيام", "probability": 0.963134765625}, {"start": 1210.49, "end": 1210.65, "word": " ولا", "probability": 0.89208984375}, {"start": 1210.65, "end": 1211.15, "word": " لأ؟", "probability": 0.93212890625}], "temperature": 1.0}, {"id": 55, "seek": 122885, "start": 1211.62, "end": 1228.86, "text": "طيب holy blood using edita or heparin as anticoagulant طبعا انا عندى العينة تبعتي لازم يكون holy blood بستخدم .. يكون طبعا ال tube edita او heparin طبعا يكون فيها edita او heparin", "tokens": [9566, 1829, 3555, 10622, 3390, 1228, 1257, 2786, 420, 415, 2181, 259, 382, 2511, 2789, 559, 425, 394, 23032, 3555, 3615, 995, 1975, 8315, 43242, 7578, 18863, 9957, 3660, 6055, 3555, 3615, 31371, 5296, 31377, 2304, 7251, 30544, 10622, 3390, 4724, 14851, 9778, 40448, 4386, 7251, 30544, 23032, 3555, 3615, 995, 2423, 9917, 1257, 2786, 1975, 2407, 415, 2181, 259, 23032, 3555, 3615, 995, 7251, 30544, 8978, 11296, 1257, 2786, 1975, 2407, 415, 2181, 259], "avg_logprob": -0.15810032522207812, "compression_ratio": 1.564102564102564, "no_speech_prob": 5.960464477539062e-07, "words": [{"start": 1211.62, "end": 1212.24, "word": "طيب", "probability": 0.9384765625}, {"start": 1212.24, "end": 1212.84, "word": " holy", "probability": 0.775390625}, {"start": 1212.84, "end": 1213.18, "word": " blood", "probability": 0.95556640625}, {"start": 1213.18, "end": 1213.68, "word": " using", "probability": 0.95166015625}, {"start": 1213.68, "end": 1214.18, "word": " edita", "probability": 0.6025390625}, {"start": 1214.18, "end": 1214.48, "word": " or", "probability": 0.896484375}, {"start": 1214.48, "end": 1215.06, "word": " heparin", "probability": 0.9130859375}, {"start": 1215.06, "end": 1215.28, "word": " as", "probability": 0.97216796875}, {"start": 1215.28, "end": 1216.12, "word": " anticoagulant", "probability": 0.93515625}, {"start": 1216.12, "end": 1216.6, "word": " طبعا", "probability": 0.8743896484375}, {"start": 1216.6, "end": 1217.38, "word": " انا", "probability": 0.7255859375}, {"start": 1217.38, "end": 1218.24, "word": " عندى", "probability": 0.689208984375}, {"start": 1218.24, "end": 1218.84, "word": " العينة", "probability": 0.84521484375}, {"start": 1218.84, "end": 1219.38, "word": " تبعتي", "probability": 0.822265625}, {"start": 1219.38, "end": 1220.04, "word": " لازم", "probability": 0.9957682291666666}, {"start": 1220.04, "end": 1220.3, "word": " يكون", "probability": 0.9931640625}, {"start": 1220.3, "end": 1220.6, "word": " holy", "probability": 0.9365234375}, {"start": 1220.6, "end": 1221.04, "word": " blood", "probability": 0.9580078125}, {"start": 1221.04, "end": 1222.12, "word": " بستخدم", "probability": 0.941162109375}, {"start": 1222.12, "end": 1222.34, "word": " ..", "probability": 0.429443359375}, {"start": 1222.34, "end": 1223.02, "word": " يكون", "probability": 0.915771484375}, {"start": 1223.02, "end": 1223.44, "word": " طبعا", "probability": 0.965087890625}, {"start": 1223.44, "end": 1223.56, "word": " ال", "probability": 0.489990234375}, {"start": 1223.56, "end": 1224.36, "word": " tube", "probability": 0.164306640625}, {"start": 1224.36, "end": 1224.94, "word": " edita", "probability": 0.923095703125}, {"start": 1224.94, "end": 1225.32, "word": " او", "probability": 0.905029296875}, {"start": 1225.32, "end": 1225.86, "word": " heparin", "probability": 0.9650065104166666}, {"start": 1225.86, "end": 1226.5, "word": " طبعا", "probability": 0.8258056640625}, {"start": 1226.5, "end": 1227.14, "word": " يكون", "probability": 0.965087890625}, {"start": 1227.14, "end": 1227.46, "word": " فيها", "probability": 0.978515625}, {"start": 1227.46, "end": 1227.88, "word": " edita", "probability": 0.97216796875}, {"start": 1227.88, "end": 1228.26, "word": " او", "probability": 0.976318359375}, {"start": 1228.26, "end": 1228.86, "word": " heparin", "probability": 0.9391276041666666}], "temperature": 1.0}, {"id": 56, "seek": 125163, "start": 1229.21, "end": 1251.63, "text": "و ممكن كمان استخدم capillary blood يعني من ال skin puncture باستخدام الثومة بيبت يعني اعبي الثومة بيبت مباشرة طيب انا ايش بلزمني؟ بلزمني بيبت تمام اما micro bibet او اللي هي ال automatic bibet اللي انتوا بتشتغلوا عليها في المختبرات او الثومة بيبت اللي مخصصة لل RBCs", "tokens": [2407, 3714, 43020, 9122, 2304, 7649, 44713, 9778, 40448, 1410, 46367, 3390, 37495, 22653, 9154, 2423, 3178, 27006, 540, 4724, 995, 14851, 9778, 3215, 10943, 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"probability": 0.9695638020833334}, {"start": 1246.39, "end": 1246.61, "word": " عليها", "probability": 0.92041015625}, {"start": 1246.61, "end": 1246.69, "word": " في", "probability": 0.7568359375}, {"start": 1246.69, "end": 1247.39, "word": " المختبرات", "probability": 0.9859619140625}, {"start": 1247.39, "end": 1247.95, "word": " او", "probability": 0.90234375}, {"start": 1247.95, "end": 1248.45, "word": " الثومة", "probability": 0.8743896484375}, {"start": 1248.45, "end": 1248.75, "word": " بيبت", "probability": 0.986572265625}, {"start": 1248.75, "end": 1248.89, "word": " اللي", "probability": 0.919677734375}, {"start": 1248.89, "end": 1249.71, "word": " مخصصة", "probability": 0.98173828125}, {"start": 1249.71, "end": 1250.35, "word": " لل", "probability": 0.451171875}, {"start": 1250.35, "end": 1251.63, "word": " RBCs", "probability": 0.8030598958333334}], "temperature": 1.0}, {"id": 57, "seek": 126816, "start": 1251.84, "end": 1268.16, "text": "تفتذكروا هاي الصورة اللي احنا ذكرنا .. عرضنا فيها ثومة بيبت حكينا انه في عندي ثومة بيبت خاصة بال white pieces count و ثومة بيبت خاصة بال R pieces count تمام؟", "tokens": [2655, 5172, 2655, 8848, 37983, 14407, 8032, 47302, 31767, 13063, 3660, 13672, 1829, 1975, 5016, 8315, 29910, 37983, 8315, 4386, 6225, 43042, 8315, 8978, 11296, 38637, 20498, 3660, 4724, 1829, 3555, 2655, 11331, 4117, 1829, 8315, 16472, 3224, 8978, 18871, 16254, 38637, 20498, 3660, 4724, 1829, 3555, 2655, 16490, 33546, 3660, 20666, 2418, 3755, 1207, 4032, 38637, 20498, 3660, 4724, 1829, 3555, 2655, 16490, 33546, 3660, 20666, 497, 3755, 1207, 46811, 10943, 22807], "avg_logprob": -0.20703125402734085, "compression_ratio": 1.6710526315789473, "no_speech_prob": 1.7881393432617188e-07, "words": [{"start": 1251.84, "end": 1252.5, "word": "تفتذكروا", "probability": 0.6834920247395834}, {"start": 1252.5, "end": 1252.84, "word": " هاي", "probability": 0.8349609375}, {"start": 1252.84, "end": 1253.88, "word": " الصورة", "probability": 0.9899088541666666}, 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.. ال .. ال .. ال .. ال .. ال .. ال .. ال .. ال .. ال .. ال .. ال .. ال .. ال .. ال .. ال .. ال .. ال .. ال .. ال .. ال .. ال .. ال .. ال .. ال .. ال .. ال .. ال .. ال .. ال .. ال .. ال .. ال .. ال .. ال .. ال .. ال .. ال .. ال .. ال .. ال .. ال .. ال .. ال .. ال .. ال .. ال .. ال .. ال .. ال .. ال .. ال .. ال .. ال .. ال .. ال .. ال .. ال .. ال .. ال .. ال .. ال .. ال .. ال .. ال .. ال .. ال .. ال .. ال .. ال .. ال .. ال .. ال .. ال .. ال .. ال .. ال .. ال .. ال .. ال .. ال .. ال .. ال .. ال .. ال .. ال .. ال .. ال .. ال .. ال .. ال .. ال .. ال", "tokens": [42213, 38149, 8978, 11296, 32748, 2304, 13672, 1829, 10874, 2304, 34268, 3224, 9154, 2423, 13063, 25708, 6225, 42213, 2655, 11983, 4032, 4724, 16254, 2655, 5551, 3615, 21292, 2423, 4386, 23032, 3555, 3615, 995, 2423, 4386, 2423, 4386, 2423, 4386, 2423, 4386, 2423, 4386, 2423, 4386, 2423, 4386, 2423, 4386, 2423, 4386, 2423, 4386, 2423, 4386, 2423, 4386, 2423, 4386, 2423, 4386, 2423, 4386, 2423, 4386, 2423, 4386, 2423, 4386, 2423, 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"word": " احضر", "probability": 0.9686279296875}, {"start": 1533.96, "end": 1534.84, "word": " واحد", "probability": 0.988525390625}, {"start": 1534.84, "end": 1535.56, "word": " ل", "probability": 0.9501953125}, {"start": 1535.56, "end": 1536.18, "word": " ..", "probability": 0.482421875}, {"start": 1536.18, "end": 1536.56, "word": " ل", "probability": 0.6015625}, {"start": 1536.56, "end": 1537.12, "word": " dilution", "probability": 0.922119140625}, {"start": 1537.12, "end": 1537.54, "word": " طبعا", "probability": 0.9910888671875}, {"start": 1537.54, "end": 1537.76, "word": " اللي", "probability": 0.95751953125}, {"start": 1537.76, "end": 1538.0, "word": " هو", "probability": 0.9912109375}, {"start": 1538.0, "end": 1538.64, "word": " متين", "probability": 0.910888671875}, {"start": 1538.64, "end": 1538.82, "word": " و", "probability": 0.71484375}, {"start": 1538.82, "end": 1539.14, "word": " ال", "probability": 0.90087890625}, {"start": 1539.14, "end": 1539.4, "word": " total", "probability": 0.9052734375}, {"start": 1539.4, "end": 1540.88, "word": " متين", "probability": 0.8818359375}, {"start": 1540.88, "end": 1541.48, "word": " واحد", "probability": 0.91455078125}, {"start": 1541.48, "end": 1542.02, "word": " طيب", "probability": 0.98291015625}, {"start": 1542.02, "end": 1542.32, "word": " جدش", "probability": 0.7496744791666666}, {"start": 1542.32, "end": 1542.54, "word": " بدي", "probability": 0.937744140625}, {"start": 1542.54, "end": 1542.84, "word": " احط", "probability": 0.82470703125}, {"start": 1542.84, "end": 1543.0, "word": " لو", "probability": 0.78759765625}, {"start": 1543.0, "end": 1543.76, "word": " حطيت", "probability": 0.8123372395833334}, {"start": 1543.76, "end": 1544.06, "word": " هنا", "probability": 0.99462890625}], "temperature": 1.0}, {"id": 71, "seek": 156385, "start": 1545.61, "end": 1563.85, "text": "لو حطيت من ال blood عشرين microliter تمام؟ هيكون في ال .. ال .. هيكون في ال tube .. اه و هستخدم diluent عبارة عن ال volume تبعه", "tokens": [1211, 2407, 11331, 9566, 36081, 9154, 2423, 3390, 6225, 46309, 9957, 4532, 40632, 46811, 10943, 22807, 39896, 30544, 8978, 2423, 4386, 2423, 4386, 39896, 30544, 8978, 2423, 9917, 4386, 1975, 3224, 4032, 8032, 14851, 9778, 40448, 11504, 19226, 6225, 3555, 9640, 3660, 18871, 2423, 5523, 6055, 3555, 3615, 3224], "avg_logprob": -0.12609374463558198, "compression_ratio": 1.35, "no_speech_prob": 2.6226043701171875e-06, "words": [{"start": 1545.61, "end": 1545.87, "word": "لو", "probability": 0.99267578125}, {"start": 1545.87, "end": 1546.33, "word": " حطيت", "probability": 0.9807942708333334}, {"start": 1546.33, "end": 1546.53, "word": " من", "probability": 0.99169921875}, {"start": 1546.53, "end": 1546.69, "word": " ال", "probability": 0.83251953125}, {"start": 1546.69, "end": 1547.17, "word": " blood", "probability": 0.92529296875}, {"start": 1547.17, "end": 1549.77, "word": " عشرين", "probability": 0.8548177083333334}, {"start": 1549.77, "end": 1550.59, "word": " microliter", 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أمقال فإذا ممكن أنا أحضر في تيوب أحط عشرين microliter من ال blood و أربعة أمقال من ال diluting fluid اللي هو ال saline طبعا طيب", "tokens": [19, 1360, 13672, 1829, 31439, 6225, 46309, 9957, 8978, 44650, 9957, 23032, 3555, 3615, 995, 13672, 1829, 31439, 6024, 114, 3615, 5172, 11933, 15730, 1975, 8315, 44713, 9778, 40448, 2655, 4386, 11933, 15730, 2423, 14407, 24401, 25961, 6225, 3555, 9640, 3660, 18871, 6225, 46309, 9957, 9673, 2655, 9957, 3714, 25720, 9154, 18863, 46309, 9957, 9122, 10943, 22807, 31104, 23032, 3555, 3615, 995, 37495, 22653, 5551, 25513, 27884, 5551, 2304, 4587, 6027, 6156, 28814, 15730, 3714, 43020, 41850, 5551, 5016, 11242, 2288, 8978, 6055, 1829, 37746, 5551, 5016, 9566, 6225, 46309, 9957, 4532, 40632, 9154, 2423, 3390, 4032, 5551, 25513, 27884, 5551, 2304, 4587, 6027, 9154, 2423, 11504, 10861, 9113, 13672, 1829, 31439, 2423, 1845, 533, 23032, 3555, 3615, 995, 23032, 1829, 3555], "avg_logprob": -0.19715447299848726, "compression_ratio": 1.9051724137931034, 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"end": 1574.09, "word": " العشرين", "probability": 0.9562174479166666}, {"start": 1574.09, "end": 1574.93, "word": " كام؟", "probability": 0.8732096354166666}, {"start": 1574.93, "end": 1575.37, "word": " 4000", "probability": 0.5986328125}, {"start": 1575.37, "end": 1576.47, "word": " طبعا", "probability": 0.925537109375}, {"start": 1576.47, "end": 1576.85, "word": " يعني", "probability": 0.797119140625}, {"start": 1576.85, "end": 1577.23, "word": " أربعة", "probability": 0.71240234375}, {"start": 1577.23, "end": 1577.63, "word": " أمقال", "probability": 0.811767578125}, {"start": 1577.63, "end": 1578.35, "word": " فإذا", "probability": 0.93798828125}, {"start": 1578.35, "end": 1578.67, "word": " ممكن", "probability": 0.841064453125}, {"start": 1578.67, "end": 1578.85, "word": " أنا", "probability": 0.59375}, {"start": 1578.85, "end": 1579.21, "word": " أحضر", "probability": 0.97509765625}, {"start": 1579.21, "end": 1579.35, "word": " في", "probability": 0.89697265625}, {"start": 1579.35, "end": 1579.57, "word": " تيوب", "probability": 0.7473958333333334}, {"start": 1579.57, "end": 1579.89, "word": " أحط", "probability": 0.8899739583333334}, {"start": 1579.89, "end": 1580.27, "word": " عشرين", "probability": 0.9720052083333334}, {"start": 1580.27, "end": 1580.81, "word": " microliter", "probability": 0.72265625}, {"start": 1580.81, "end": 1581.03, "word": " من", "probability": 0.99560546875}, {"start": 1581.03, "end": 1581.17, "word": " ال", "probability": 0.81787109375}, {"start": 1581.17, "end": 1581.47, "word": " blood", "probability": 0.953125}, {"start": 1581.47, "end": 1581.77, "word": " و", "probability": 0.8857421875}, {"start": 1581.77, "end": 1582.19, "word": " أربعة", "probability": 0.7763671875}, {"start": 1582.19, "end": 1582.79, "word": " أمقال", "probability": 0.95361328125}, {"start": 1582.79, "end": 1583.39, "word": " من", "probability": 0.998046875}, {"start": 1583.39, "end": 1584.25, "word": " ال", "probability": 0.455810546875}, {"start": 1584.25, "end": 1584.79, "word": " diluting", "probability": 0.8056640625}, {"start": 1584.79, "end": 1585.17, "word": " fluid", "probability": 0.67431640625}, {"start": 1585.17, "end": 1585.37, "word": " اللي", "probability": 0.991455078125}, {"start": 1585.37, "end": 1585.53, "word": " هو", "probability": 0.9892578125}, {"start": 1585.53, "end": 1585.71, "word": " ال", "probability": 0.888671875}, {"start": 1585.71, "end": 1586.27, "word": " saline", "probability": 0.6602783203125}, {"start": 1586.27, "end": 1587.09, "word": " طبعا", "probability": 0.9542236328125}, {"start": 1587.09, "end": 1589.29, "word": " طيب", "probability": 0.8839518229166666}], "temperature": 1.0}, {"id": 73, "seek": 162002, "start": 1590.82, "end": 1620.02, "text": "إذا ممكن أحضره إما بطريقة ثومابيبت أسحب للنص أكمل ل 101 أتخلص من أعمل mixing أتخلص من أول 3-4 قطرات إذا ال dilution 1 ل 2 ك dilution أولي طبعا أنا مابحكيش عن ال total dilution أنا بحكي نسبة ال blood إلى ال diluent لما بقول 1 ل 2 تمام؟ أو إما باستخدام ال micro pipette العادية باستخدام تيوب باخد diluent و بحط عليه ال blood", "tokens": [28814, 15730, 3714, 43020, 5551, 5016, 11242, 2288, 3224, 11933, 15042, 4724, 9566, 16572, 28671, 38637, 20498, 16758, 1829, 3555, 2655, 5551, 3794, 5016, 3555, 24976, 1863, 9381, 5551, 24793, 1211, 5296, 21055, 5551, 2655, 9778, 1211, 9381, 9154, 5551, 25957, 1211, 11983, 5551, 2655, 9778, 1211, 9381, 9154, 5551, 12610, 805, 12, 19, 12174, 9566, 2288, 9307, 11933, 15730, 2423, 11504, 1448, 502, 5296, 568, 9122, 11504, 1448, 5551, 12610, 1829, 23032, 3555, 3615, 995, 41850, 3714, 16758, 5016, 4117, 1829, 8592, 18871, 2423, 3217, 11504, 1448, 41850, 4724, 5016, 4117, 1829, 8717, 35457, 3660, 2423, 3390, 30731, 2423, 11504, 19226, 5296, 15042, 4724, 39648, 502, 5296, 568, 46811, 10943, 22807, 34051, 11933, 15042, 4724, 995, 14851, 9778, 3215, 10943, 2423, 4532, 8489, 3007, 18863, 18513, 10632, 4724, 995, 14851, 9778, 3215, 10943, 6055, 1829, 37746, 4724, 47283, 3215, 11504, 19226, 4032, 4724, 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2234.46, "end": 2234.9, "word": " ml", "probability": 0.61572265625}, {"start": 2234.9, "end": 2235.28, "word": " في", "probability": 0.8583984375}, {"start": 2235.28, "end": 2235.66, "word": " tube", "probability": 0.30712890625}], "temperature": 1.0}, {"id": 100, "seek": 225567, "start": 2237.35, "end": 2255.67, "text": "مش في الصومة بايبت make certain تكوني متأكدة انه ال بايبت ال hematocytometer and the covering glass are free from their cleaned and dried blood لازم الأدوات اللي بنستخدمها سواء كانت بايبت او hematocytometer او حتى ال cover slip ال covering glass", "tokens": [2304, 8592, 8978, 31767, 20498, 3660, 4724, 995, 1829, 3555, 2655, 652, 1629, 6055, 30544, 1829, 44650, 10721, 4117, 41891, 16472, 3224, 2423, 4724, 995, 1829, 3555, 2655, 2423, 8636, 2513, 1344, 83, 13606, 293, 264, 10322, 4276, 366, 1737, 490, 641, 596, 68, 282, 292, 293, 13538, 3390, 5296, 31377, 2304, 16247, 3215, 2407, 9307, 13672, 1829, 44945, 14851, 9778, 40448, 11296, 8608, 2407, 16606, 25961, 2655, 4724, 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الجاف ensure that the diluting fluid is free from blood، كمان ال diluting fluid اللي أنا بدى أستخدمه لازم يكون نظيف، مافيش فيه دم تمام أو أي contamination أخرى", "tokens": [1211, 31377, 2304, 7251, 30544, 16490, 6027, 1829, 9154, 6024, 118, 3555, 25720, 12399, 36632, 5551, 8592, 1829, 16606, 39894, 25957, 1211, 1975, 2655, 3794, 47283, 12399, 39894, 1211, 2655, 3794, 4587, 47356, 12399, 39894, 25957, 1211, 37495, 22653, 11933, 3615, 995, 28671, 24976, 2288, 33604, 10632, 4032, 24976, 22488, 3215, 4032, 36632, 11242, 995, 32748, 2304, 25724, 31845, 5586, 300, 264, 11504, 10861, 9113, 307, 1737, 490, 3390, 12399, 9122, 2304, 7649, 2423, 11504, 10861, 9113, 13672, 1829, 41850, 47525, 7578, 5551, 14851, 9778, 40448, 3224, 5296, 31377, 2304, 7251, 30544, 8717, 19913, 33911, 12399, 19446, 41185, 8592, 8978, 3224, 11778, 2304, 46811, 10943, 34051, 36632, 33012, 5551, 34740, 7578], "avg_logprob": -0.13999721221625805, "compression_ratio": 1.619607843137255, "no_speech_prob": 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بتعمل", "probability": 0.9899088541666666}, {"start": 2263.83, "end": 2265.57, "word": " يعني", "probability": 0.95361328125}, {"start": 2265.57, "end": 2266.21, "word": " إعاقة", "probability": 0.89892578125}, {"start": 2266.21, "end": 2266.71, "word": " للرؤية", "probability": 0.950927734375}, {"start": 2266.71, "end": 2266.83, "word": " و", "probability": 0.53271484375}, {"start": 2266.83, "end": 2267.61, "word": " للعدد", "probability": 0.7685546875}, {"start": 2267.61, "end": 2267.75, "word": " و", "probability": 0.4296875}, {"start": 2267.75, "end": 2268.19, "word": " أيضا", "probability": 0.8844401041666666}, {"start": 2268.19, "end": 2268.61, "word": " الدم", "probability": 0.968994140625}, {"start": 2268.61, "end": 2269.15, "word": " الجاف", "probability": 0.98583984375}, {"start": 2269.15, "end": 2269.89, "word": " ensure", "probability": 0.496826171875}, {"start": 2269.89, "end": 2270.25, "word": " that", "probability": 0.95361328125}, {"start": 2270.25, "end": 2270.49, "word": " the", "probability": 0.92529296875}, {"start": 2270.49, "end": 2270.97, "word": " diluting", "probability": 0.97705078125}, {"start": 2270.97, "end": 2271.43, "word": " fluid", "probability": 0.8466796875}, {"start": 2271.43, "end": 2271.67, "word": " is", "probability": 0.9765625}, {"start": 2271.67, "end": 2272.01, "word": " free", "probability": 0.9365234375}, {"start": 2272.01, "end": 2272.41, "word": " from", "probability": 0.91357421875}, {"start": 2272.41, "end": 2273.21, "word": " blood،", "probability": 0.632080078125}, {"start": 2273.21, "end": 2273.67, "word": " كمان", "probability": 0.9908854166666666}, {"start": 2273.67, "end": 2273.89, "word": " ال", "probability": 0.95947265625}, {"start": 2273.89, "end": 2274.33, "word": " diluting", "probability": 0.96728515625}, {"start": 2274.33, "end": 2274.67, "word": " fluid", "probability": 0.90625}, {"start": 2274.67, "end": 2274.81, "word": " اللي", "probability": 0.9150390625}, {"start": 2274.81, "end": 2274.95, "word": " أنا", "probability": 0.73486328125}, {"start": 2274.95, "end": 2275.11, "word": " بدى", "probability": 0.421630859375}, {"start": 2275.11, "end": 2275.61, "word": " أستخدمه", "probability": 0.879443359375}, {"start": 2275.61, "end": 2275.85, "word": " لازم", "probability": 0.98876953125}, {"start": 2275.85, "end": 2276.11, "word": " يكون", "probability": 0.98876953125}, {"start": 2276.11, "end": 2276.99, "word": " نظيف،", "probability": 0.9376220703125}, {"start": 2276.99, "end": 2277.37, "word": " مافيش", "probability": 0.8889973958333334}, {"start": 2277.37, "end": 2277.77, "word": " فيه", "probability": 0.979736328125}, {"start": 2277.77, "end": 2278.19, "word": " دم", "probability": 0.99267578125}, {"start": 2278.19, "end": 2278.91, "word": " تمام", "probability": 0.88671875}, {"start": 2278.91, "end": 2279.43, "word": " أو", "probability": 0.59326171875}, {"start": 2279.43, "end": 2279.63, "word": " أي", "probability": 0.64990234375}, {"start": 2279.63, "end": 2280.21, 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ما بين الأربعة ل .. ل الخمسة و نص ال .. ال .. ال Y .. ال RPCs وها كذا", "tokens": [2304, 3215, 31747, 9957, 11296, 37495, 22653, 1392, 23032, 3555, 3615, 995, 41850, 37893, 4386, 37893, 23032, 6027, 49401, 9154, 4117, 6055, 5016, 5172, 19913, 1829, 20666, 4386, 20666, 11242, 3555, 9566, 20666, 11242, 3555, 9566, 2423, 2710, 22526, 4724, 3794, 15844, 16247, 4587, 1211, 6055, 30544, 1829, 6225, 9640, 5172, 3660, 37495, 22653, 5551, 8592, 1829, 16606, 8978, 4386, 44945, 38251, 1829, 11296, 23867, 37495, 22653, 14739, 3224, 50113, 15040, 16070, 43761, 2423, 3655, 45841, 13496, 43242, 2423, 6556, 9154, 2423, 2975, 5296, 3705, 34051, 9154, 2975, 5296, 3499, 9154, 20984, 5296, 2975, 1360, 34051, 9154, 31104, 5296, 4386, 5296, 2975, 1360, 2423, 398, 12986, 82, 19446, 49374, 16247, 25513, 27884, 5296, 4386, 5296, 33962, 2304, 3794, 3660, 4032, 8717, 9381, 2423, 4386, 2423, 4386, 2423, 398, 4386, 2423, 497, 12986, 82, 4032, 11296, 9122, 15730], "avg_logprob": -0.20323822830898175, 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{"start": 2536.76, "end": 2537.14, "word": " 14", "probability": 0.8876953125}, {"start": 2537.14, "end": 2538.96, "word": " من", "probability": 0.9443359375}, {"start": 2538.96, "end": 2539.42, "word": " 3000", "probability": 0.8466796875}, {"start": 2539.42, "end": 2539.64, "word": " ل", "probability": 0.96240234375}, {"start": 2539.64, "end": 2540.16, "word": " 11000", "probability": 0.860595703125}, {"start": 2540.16, "end": 2540.58, "word": " أو", "probability": 0.56689453125}, {"start": 2540.58, "end": 2540.76, "word": " من", "probability": 0.97265625}, {"start": 2540.76, "end": 2541.22, "word": " 4000", "probability": 0.93798828125}, {"start": 2541.22, "end": 2542.0, "word": " ل", "probability": 0.98193359375}, {"start": 2542.0, "end": 2542.4, "word": " ..", "probability": 0.65625}, {"start": 2542.4, "end": 2542.54, "word": " ل", "probability": 0.8671875}, {"start": 2542.54, "end": 2543.16, "word": " 11000", "probability": 0.926513671875}, {"start": 2543.16, "end": 2543.38, 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39896, 4117, 44945, 30544, 4032, 36520, 8315, 32767, 5016, 46958, 43500, 8315, 4386, 5296, 1863, 11296, 10632, 3714, 5016, 46958, 43500, 8315, 5296, 20292, 20498, 5551, 39237, 1863, 7578, 36145, 11296, 6055, 30544, 4724, 3794, 1829, 9566, 3660, 25894, 24793, 37495, 9566, 1829, 24793, 5551, 46538, 6225, 31845, 10632], "avg_logprob": -0.11615565756581864, "compression_ratio": 1.4838709677419355, "no_speech_prob": 1.5497207641601562e-06, "words": [{"start": 2554.85, "end": 2555.69, "word": "تمام؟", "probability": 0.8046875}, {"start": 2555.69, "end": 2556.53, "word": " هيك", "probability": 0.927978515625}, {"start": 2556.53, "end": 2556.87, "word": " بنكون", "probability": 0.9794921875}, {"start": 2556.87, "end": 2557.25, "word": " وصلنا", "probability": 0.98388671875}, {"start": 2557.25, "end": 2558.19, "word": " لمحاضرتنا", "probability": 0.882421875}, {"start": 2558.19, "end": 2558.75, "word": " ..", "probability": 0.78369140625}, {"start": 2558.75, "end": 2559.15, "word": " لنهاية", 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\ No newline at end of file
diff --git a/PL9fwy3NUQKwY9jl8xw-bWkVZkWcGGyspd/FJ1Ngmal9to_raw.srt b/PL9fwy3NUQKwY9jl8xw-bWkVZkWcGGyspd/FJ1Ngmal9to_raw.srt
new file mode 100644
index 0000000000000000000000000000000000000000..7bfd86b27a3d99e0b5210bfbf0639035189b13ac
--- /dev/null
+++ b/PL9fwy3NUQKwY9jl8xw-bWkVZkWcGGyspd/FJ1Ngmal9to_raw.srt
@@ -0,0 +1,2268 @@
+1
+00:00:06,630 --> 00:00:10,490
+السلام عليكم ورحمة الله وبركاته طالبات العزيزات
+
+2
+00:00:10,490 --> 00:00:15,050
+اليوم ان شاء الله راح نشرح محاضرة بعنوان red blood
+
+3
+00:00:15,050 --> 00:00:19,530
+cell count RBC count في نهاية هذه المحاضرة راح
+
+4
+00:00:19,530 --> 00:00:25,070
+نكون قادرين بإذن الله انه نتعرف على طريقة لعد
+
+5
+00:00:25,070 --> 00:00:31,310
+خلايا الدمطريقة manual يدوية إنه كيف بنعد خلايا
+
+6
+00:00:31,310 --> 00:00:37,310
+الدم الحمراء باستخدام ال chamber شريحة ومخصصة لعد
+
+7
+00:00:37,310 --> 00:00:41,930
+الخلايا وباستخدام ال microscope وكيف بنعمل
+
+8
+00:00:41,930 --> 00:00:45,370
+dilution ل blood لحتى نتمكن من عد خلايا الدم
+
+9
+00:00:45,370 --> 00:00:49,740
+الحمراءمعلومات مشابهة لما ذكرناها بال white blood
+
+10
+00:00:49,740 --> 00:00:55,340
+cell count ولكن مع اختلاف في بعض التفاصيل خلينا في
+
+11
+00:00:55,340 --> 00:01:02,300
+البداية نذكر كتقديم بسيط للموضوع نذكر أهمية ال ..
+
+12
+00:01:02,300 --> 00:01:08,020
+ال red blood cells ووظائفهاRed blood cell make up
+
+13
+00:01:08,020 --> 00:01:14,540
+almost 45% of the blood volume تقريبا تحت الخلايا
+
+14
+00:01:14,540 --> 00:01:19,980
+الدم الحمراء حجم 45% من حجم الدم ككل في بعض
+
+15
+00:01:19,980 --> 00:01:24,780
+المراجع ذكرنا أنه 44% على أساس أن ال buffy coat 1%
+
+16
+00:01:25,150 --> 00:01:32,050
+طبعا مش خلال 45% او 44% المهم انها بتحتل جزء ..
+
+17
+00:01:32,050 --> 00:01:38,790
+خلينا نحكي تقريبا 45% من حجم الدم ككل their
+
+18
+00:01:38,790 --> 00:01:44,450
+primary function is to carry oxygen وظيفة الخلايا
+
+19
+00:01:44,450 --> 00:01:49,830
+الدم الحمراء الأساسية طبعا انها تحمل ال oxygen من
+
+20
+00:01:49,830 --> 00:01:54,010
+وين؟ من ال lungلوين؟ to the every cell in the body
+
+21
+00:01:54,010 --> 00:01:58,310
+ل البلد tissue ل البلد organs بتوصل ال oxygen
+
+22
+00:01:58,310 --> 00:02:03,290
+تمام؟ Red blood cell الخلايا الدم الحمراء composed
+
+23
+00:02:03,290 --> 00:02:07,650
+predominantly of a protein and iron compound طبعا
+
+24
+00:02:07,650 --> 00:02:15,930
+هي الجزء او المساحة العظمى من ال RBCs موجود فيها
+
+25
+00:02:15,930 --> 00:02:20,570
+او بيحتلها homoglobinموجود فيها بروتين هو عبارة عن
+
+26
+00:02:20,570 --> 00:02:26,210
+iron containing بروتين بروتين يحمل ذرات ال iron
+
+27
+00:02:26,210 --> 00:02:30,630
+هذا البروتين هو ال homoglobin ال homoglobin هو
+
+28
+00:02:30,630 --> 00:02:35,830
+الجزء او البروتين المسئول عن حمل ال oxygen و نقله
+
+29
+00:02:35,830 --> 00:02:42,150
+حكينا انه من خصائصه انه بيحمل او بيتلقى oxygen في
+
+30
+00:02:42,150 --> 00:02:45,690
+المناطق اللي بيكون فيها ال oxygen pressure عالي زي
+
+31
+00:02:45,690 --> 00:02:51,090
+ال lung تمام؟وبيترك ال oxygen release تمام؟ يترك
+
+32
+00:02:51,090 --> 00:02:54,690
+ال oxygen أو release ال oxygen في المناطق اللي
+
+33
+00:02:54,690 --> 00:02:58,590
+بيكون فيها ال oxygen pressure منخفض زي ان ال
+
+34
+00:02:58,590 --> 00:03:01,610
+tissues و ال organs و ال blood cells every blood
+
+35
+00:03:01,610 --> 00:03:07,740
+cells تمام؟ال .. ال homoglobin هو اللي لونه أحمر
+
+36
+00:03:07,740 --> 00:03:13,640
+لإنه بيحتوي على ال iron تمام فبالتالي هو اللي يكسب
+
+37
+00:03:13,640 --> 00:03:18,540
+خلايا الدم لونها الأحمر يكسب خلايا الدم الحمراء
+
+38
+00:03:18,540 --> 00:03:23,620
+طبعا لونها الأحمر the blood vessels through body
+
+39
+00:03:23,620 --> 00:03:27,760
+tissueshomoglobin then release the oxygen to the
+
+40
+00:03:27,760 --> 00:03:31,840
+cell or the body حكينا إنه هو بيحمل ال oxygen من
+
+41
+00:03:31,840 --> 00:03:37,820
+ال lung تمام أثناء مرور الدم عبر الرقا تمام بيحمل
+
+42
+00:03:37,820 --> 00:03:41,920
+ال oxygen لإن ضغط ال oxygen في ال lung عاليو as
+
+43
+00:03:41,920 --> 00:03:46,120
+blood pass through the body لما ال .. ال .. الدم
+
+44
+00:03:46,120 --> 00:03:51,360
+بي .. بي circulate طبعا، بي .. بيدور .. بي ..
+
+45
+00:03:51,360 --> 00:03:54,640
+بينتقل الى ال tissue و بيروح يغذي ال tissue يعنى،
+
+46
+00:03:54,640 --> 00:04:00,520
+طبعا فبالتالي هو في هذه المرحلة بيوصل oxygen، it
+
+47
+00:04:00,520 --> 00:04:05,100
+releasethe oxygen، تمام؟ red blood cells are so
+
+48
+00:04:05,100 --> 00:04:09,000
+packed with homoglobin، حكينا إنها هي معبقة،
+
+49
+00:04:09,000 --> 00:04:14,640
+مملوقة بالهمoglobin، بيشغل حجم كتير كبير من الحجم
+
+50
+00:04:14,640 --> 00:04:18,740
+الكل لخلية الدم الحمراء، ال homoglobinincluding
+
+51
+00:04:18,740 --> 00:04:23,740
+طبعا هذا بيخلي ال RBCs lack .. lack money
+
+52
+00:04:23,740 --> 00:04:28,220
+component طبعا تفتقر او انها تفتقد كثير من
+
+53
+00:04:28,220 --> 00:04:32,640
+المكونات اللي موجودة في الخلايا الأخرى او الخلايا
+
+54
+00:04:32,640 --> 00:04:36,900
+الطبيعية العادية يعني مثلا خلية الدم الحمراء
+
+55
+00:04:36,900 --> 00:04:41,160
+ماعندهاش nucleus ماعندهاش organelles طبعا؟ ليش؟
+
+56
+00:04:41,160 --> 00:04:45,640
+لإن لحتىاتمكنها من حمل كمية كافية من ال
+
+57
+00:04:45,640 --> 00:04:49,360
+homoglobin، مافيش nucleus، مافيش organelles علشان
+
+58
+00:04:49,360 --> 00:04:55,760
+الخلية تحمل homoglobin بالقدر الكافي اللي يمكنها
+
+59
+00:04:55,760 --> 00:05:00,940
+من توصيل ال oxygen إلى كل خلية داخل جسم الإنسان
+
+60
+00:05:02,620 --> 00:05:07,720
+RBCs structure the membrane or out layer of the
+
+61
+00:05:07,720 --> 00:05:11,560
+red blood cell is flexible تمام؟ Like a soup
+
+62
+00:05:11,560 --> 00:05:16,760
+bubble هلأ الخلايا أو ال membrane، الغلاف تبعي
+
+63
+00:05:16,760 --> 00:05:23,520
+خلايا ال RBCsهو غلاف مرن جدا، flexible، تمام؟ مرن
+
+64
+00:05:23,520 --> 00:05:27,720
+جدا، يعني بيشبهوها بفقاعة صابون، شوفنا كيف لعبة
+
+65
+00:05:27,720 --> 00:05:31,240
+الفقاعات هاي اللي بتطلع منها الفقاعة من حلقة
+
+66
+00:05:31,240 --> 00:05:35,610
+دائرية، بنحط ميه و صابون في العلبة و ..و من خلال
+
+67
+00:05:35,610 --> 00:05:38,850
+النفخ عليها بتطلع فقاعة صبون شفتوا كيف فقاعة
+
+68
+00:05:38,850 --> 00:05:42,710
+الصبون بالأول بتتشكل و بتنحني لحد بعد ما تطلع من
+
+69
+00:05:42,710 --> 00:05:46,810
+الإطار ال RBCs زي هات يعني نفس .. بيشبهوها نفس
+
+70
+00:05:46,810 --> 00:05:51,810
+المرونة ليش؟ لإن ال RBCs bend able to bend ممكن
+
+71
+00:05:51,810 --> 00:05:56,890
+إنها تنحني أو تلتف على نفسها أو يعني يصير فيها
+
+72
+00:05:56,890 --> 00:06:02,420
+انثناقات تمام؟و بدون ما تنكسر without breaking
+
+73
+00:06:02,420 --> 00:06:06,220
+طبعا .. without breaking وين طبعا بيصير فيها هاي
+
+74
+00:06:06,220 --> 00:06:10,320
+الانحناقات و السهولة
+
+75
+00:06:10,320 --> 00:06:14,120
+و المرونة فى الحركة فى لما بدها تفوت فى ال blood
+
+76
+00:06:14,120 --> 00:06:16,860
+vessels اللى حجمها صغير فى ال capillaries الشعيرات
+
+77
+00:06:16,860 --> 00:06:20,500
+الدموية الدقيقة جدا لما بتفوت أحيانا تضطر ان هي
+
+78
+00:06:20,500 --> 00:06:25,100
+تتنحالها علشان تروح توصل oxygen طبعا او انها ..
+
+79
+00:06:25,100 --> 00:06:29,720
+يعني يصير فيها انحناقات تلتوي تلتف على نفسهافي
+
+80
+00:06:29,720 --> 00:06:34,180
+أيضًا غير الأوعية الدموية الضيقة على التفافات
+
+81
+00:06:34,180 --> 00:06:37,360
+والانحناقات
+
+82
+00:06:37,360 --> 00:06:41,840
+اللي موجودة أيضًا في الأوعية الدموية برضه كمان
+
+83
+00:06:41,840 --> 00:06:47,980
+بتكون ال RBCs قادرة على أنها تفني نفسها و تفوت
+
+84
+00:06:47,980 --> 00:06:51,400
+لحتى توصل أكسوجين everywhere this is important
+
+85
+00:06:51,400 --> 00:06:56,330
+طبعًا هي خاصية كتير مهمة في خلايا الدم الحمراءليش؟
+
+86
+00:06:56,330 --> 00:07:00,050
+لإن هي بتمكنها able to pass through the thinnest
+
+87
+00:07:00,050 --> 00:07:05,710
+blood vessels هي بتمكنها من إنها تتعبر من خلال
+
+88
+00:07:05,710 --> 00:07:09,730
+يعني أدق خلاء الأوعية الدموية thinnest blood
+
+89
+00:07:09,730 --> 00:07:12,190
+vessels يعني أدق الأوعية الدموية اللي هي
+
+90
+00:07:12,190 --> 00:07:16,310
+الكابيلاريز الشعيرات طبعا لحتى توصل ال oxygen to
+
+91
+00:07:16,310 --> 00:07:19,910
+deliver oxygen whenever it is needed في أي مكان
+
+92
+00:07:19,910 --> 00:07:24,400
+احنا محتاجينه طبعا بتوصل هذا ال oxygenخلّونا بس
+
+93
+00:07:24,400 --> 00:07:29,700
+برضه كمان نربط خاصية ال flexibility بال fragility
+
+94
+00:07:29,700 --> 00:07:35,700
+لل RBCs هي أي نعم ال RBCs مرنة و بإمكانها إنها هي
+
+95
+00:07:35,700 --> 00:07:39,740
+تلتف على نفسها يصير في انحناقات انثناقات في ال
+
+96
+00:07:39,740 --> 00:07:46,780
+membrane و تروح يعني تتعبر نفسها أو تتمرر نفسها من
+
+97
+00:07:46,780 --> 00:07:51,260
+خلال capillaries ضيقة جدا لحتى توصي ال oxygen إلا
+
+98
+00:07:51,260 --> 00:07:56,980
+إنهأيضًا الخلايا الـ RBCs تُعتبر إلى حد ما .. إلى
+
+99
+00:07:56,980 --> 00:08:02,100
+حد ما تُعتبر fragile تُعتبر هشة سهلة الكسر لهيك
+
+100
+00:08:02,100 --> 00:08:05,840
+إحنا لما بنحكيلكم اصحى بيعين الدم عوّلي تعملي
+
+101
+00:08:05,840 --> 00:08:10,220
+gentle mixing ديري بالك الخلايا تتكسر لإنها
+
+102
+00:08:10,220 --> 00:08:16,020
+mechanicalي مع الحركة القوية أو مع السوق التعامل
+
+103
+00:08:16,020 --> 00:08:20,870
+ممكن إنها أيضًا تتكسر هاي الخلاياthe capillaries
+
+104
+00:08:20,870 --> 00:08:25,250
+are so narrow تمام الأوعية الدموية الضيقة هاي the
+
+105
+00:08:25,250 --> 00:08:29,230
+thinnest blood vessels الأشعيرات الدموية are so
+
+106
+00:08:29,230 --> 00:08:35,030
+narrow ضيقة جدا to the limit that لحد ان ال RBCs
+
+107
+00:08:35,030 --> 00:08:38,610
+اللي بشكلها الطبيعي عاملة زي القرص او زي ال bacon
+
+108
+00:08:38,610 --> 00:08:45,330
+cap shape لازم انها must bend and twist to pass
+
+109
+00:08:45,330 --> 00:08:49,810
+through them علشان تتعدي من خلالهم تمام؟bend and
+
+110
+00:08:49,810 --> 00:08:54,250
+twist معناها إنها يعني تنحني وتلتف، هيك معناها،
+
+111
+00:08:54,250 --> 00:08:58,050
+طبعا، طيب الشكل الطبيعي لل RBCs إذا أنا جيت ألقيت
+
+112
+00:08:58,050 --> 00:09:02,250
+عليها نظرة علوية، طبعا، لو كانت ال RBCs موجودة على
+
+113
+00:09:02,250 --> 00:09:05,790
+شريحة و أنا جيت و طلعت عليها من فوق، بشوفها ..
+
+114
+00:09:05,790 --> 00:09:08,470
+بشوف ال top view تبعها إن هي عبارة عن زي ال discs
+
+115
+00:09:08,470 --> 00:09:13,250
+زي القرص، طبعا، بنشوفها circular أو discotheque،
+
+116
+00:09:13,250 --> 00:09:16,850
+طبعاcircular أو discotheque خلينا نكتب كلمة
+
+117
+00:09:16,850 --> 00:09:19,530
+discotheque عشان ما تكونش غريبة عليكم،
+
+118
+00:09:19,530 --> 00:09:27,890
+discotheque، تمام؟ أو بنسميها disc
+
+119
+00:09:27,890 --> 00:09:33,810
+shape، تمام؟ زي شكل القرص، discotheque أو disc
+
+120
+00:09:33,810 --> 00:09:39,490
+shapeطيب انا لو جيت اطلعت من الجانب على خلايا ال
+
+121
+00:09:39,490 --> 00:09:43,470
+RBC's ايش بشوفها؟ طب لو ان احنا جينا هنا .. انتوا
+
+122
+00:09:43,470 --> 00:09:47,550
+شايفين هنا زي في .. زي كأنه حفرة او ايشي ضيق ..
+
+123
+00:09:47,550 --> 00:09:50,870
+ايشي داخل لجوا في الخلية لو جيت انا اطلعت من
+
+124
+00:09:50,870 --> 00:09:54,690
+الجانب من هنابشوف في عندي انتثاح و انتثاح و في
+
+125
+00:09:54,690 --> 00:09:59,450
+المصمنطقة ضيقة اللي هي بسميها biconcave shape
+
+126
+00:09:59,450 --> 00:10:04,730
+مقعرة الوجهين، من هنا مقعر و من هنا مقعر، تمام؟
+
+127
+00:10:04,730 --> 00:10:09,930
+إذا مقعرة الوجهين، هي مقعر و أيضا مقعر آخر فبسميها
+
+128
+00:10:09,930 --> 00:10:15,960
+biconcave shape، تمام؟إذا ال top view، النظرة
+
+129
+00:10:15,960 --> 00:10:19,800
+العلوية لل RBCs بشوفها circular، بشوفها
+
+130
+00:10:19,800 --> 00:10:24,760
+discotheque، disc shape تمام؟ أما النظرة الجانبية
+
+131
+00:10:24,760 --> 00:10:30,820
+لل RBCs فبشوفها عبارة عن biconcave تمام؟ بشوفها
+
+132
+00:10:30,820 --> 00:10:36,380
+biconcave يعني مقعرة الوجهين concave top تمام؟ and
+
+133
+00:10:36,380 --> 00:10:43,780
+bottomطيب Erythrocyte count طبعا أنا ب .. ب ..
+
+134
+00:10:43,780 --> 00:10:49,940
+يعني بعدي الخلايا per microliter تمام؟ يعني وحدة
+
+135
+00:10:49,940 --> 00:10:55,180
+القياس سل per microliter أو سل per millimeter مكعب
+
+136
+00:10:55,180 --> 00:10:57,840
+و هذا الكلام ذكرناه في محاضرة ال white pieces
+
+137
+00:10:57,840 --> 00:11:03,870
+countنيجي لل normal ranges of RBCs المال الوضره
+
+138
+00:11:03,870 --> 00:11:09,930
+الطبيعى من 4.2 ل 5.4 مليون خلية حكينا عددها
+
+139
+00:11:09,930 --> 00:11:16,890
+بالملايين ال female 3.6 ل 5 و ال newborn 5.5 ل 6.5
+
+140
+00:11:16,890 --> 00:11:20,250
+طبعا صيرنا بنعرف ليش ال newborn عنده خلايا RBCs
+
+141
+00:11:20,250 --> 00:11:24,150
+اكبر او اكثر و صيرنا بنعرف ليش المال عنده خلايا
+
+142
+00:11:24,150 --> 00:11:28,650
+RBCs اكثر من ال female تمام؟هذه عوامل أو أسباب
+
+143
+00:11:28,650 --> 00:11:33,550
+احنا ذكرناها سابقا وصرنا ايضا بنعرف ان زيادة
+
+144
+00:11:33,550 --> 00:11:38,210
+الارثروسايت count تمام لما يكون عندى زيادة فى عدد
+
+145
+00:11:38,210 --> 00:11:43,410
+ال RBCs زيادة فى حجم ال RBCs ال hematocrit زيادة
+
+146
+00:11:43,410 --> 00:11:46,510
+فى ال homoglobin هذه كلها بسميها polycythemia
+
+147
+00:11:47,190 --> 00:11:52,870
+مؤشرات على polycythemia أما نقص عدد ال RBCs، نقص
+
+148
+00:11:52,870 --> 00:11:55,790
+ال hematocrit اللي هو الحجم اللي تحتله ال RBCs،
+
+149
+00:11:55,790 --> 00:12:00,750
+نقص ال hemoglobin بسميه أنيميا، تمام؟ بسميه أنيميا
+
+150
+00:12:00,750 --> 00:12:04,830
+يعني فقرة دم، وحكينا إن فيلة ال polycythemia حالات
+
+151
+00:12:04,830 --> 00:12:11,900
+و أيضا فيلة الأنيميا أنواعطيب erythrocyte count
+
+152
+00:12:11,900 --> 00:12:16,660
+the principle مبدأ عمل ال erythrocyte count in
+
+153
+00:12:16,660 --> 00:12:21,040
+order to facilitate RBCs count a specified volume
+
+154
+00:12:21,040 --> 00:12:24,800
+of blood is diluted with a specified volume of
+
+155
+00:12:24,800 --> 00:12:31,180
+isotonic fluidطبعا أنا بدي أاخد حجم من الدم طبعا و
+
+156
+00:12:31,180 --> 00:12:37,220
+holy blood لإنه أنا بشتغل على الخلايا هلأ و لازم
+
+157
+00:12:37,220 --> 00:12:42,800
+أخففه بحجم معين من ال daily one نفس فكرة ال white
+
+158
+00:12:42,800 --> 00:12:47,420
+pieces countتمام؟ وكنت باخد حجم من الدم و باخد حجم
+
+159
+00:12:47,420 --> 00:12:51,140
+من ال diligent طيب خصائص ال diligent كان في ال
+
+160
+00:12:51,140 --> 00:12:56,940
+white pieces count انه لازم يكسرلي ال .. ال RPCs و
+
+161
+00:12:56,940 --> 00:13:02,140
+يحفظلي ال .. ال white pieces و يصبغها علشان أشوفها
+
+162
+00:13:02,140 --> 00:13:08,360
+تمام؟ لكن هنا أنا صح بعمل dilution باخد حجم من ال
+
+163
+00:13:08,360 --> 00:13:14,650
+blood تمام؟ و حجم من ال diligent ولكنلازم يكون ال
+
+164
+00:13:14,650 --> 00:13:19,490
+diligent isotonic fluid وطبعا احنا في محاضرة من
+
+165
+00:13:19,490 --> 00:13:23,450
+المحاضرات فرقنا اش يعني hypotonic؟ اش يعني
+
+166
+00:13:23,450 --> 00:13:27,250
+hypertonic؟ واش يعني isotonic؟ وحكينا ان ال
+
+167
+00:13:27,250 --> 00:13:31,570
+isotonic هو اللي بيحافظ على شكل الخلايا مابيخليهاش
+
+168
+00:13:31,570 --> 00:13:37,050
+تتكسر يصير لها lysis او swelling او حتى كمان
+
+169
+00:13:37,050 --> 00:13:41,340
+مابيصيرلهاش shrinkageتمام؟ فإذا هو ال .. ال
+
+170
+00:13:41,340 --> 00:13:44,840
+diligent لازم يكون من خصائص ان هو isotonic يعني ال
+
+171
+00:13:44,840 --> 00:13:47,700
+.. الضغط الإسموزي تبعه مضبوط يحافظ على ضغط
+
+172
+00:13:47,700 --> 00:13:51,340
+الإسموزي للخلايا تمام؟ فبالتالي نسبة الأملح فيه
+
+173
+00:13:51,340 --> 00:13:55,580
+مضبوطة حتى .. حتى يحافظ على ضغط الإسموزي للخلايا
+
+174
+00:13:55,580 --> 00:14:02,250
+وبالتالي يحافظ على شكل الخلاياأيضا هناك شروط أخرى
+
+175
+00:14:02,250 --> 00:14:07,030
+لازم تتوفر في red cell diluting fluid إيش هي
+
+176
+00:14:07,030 --> 00:14:10,790
+الشروط اللي لازم تتوفر في ال diluent اللي أنا بدي
+
+177
+00:14:10,790 --> 00:14:15,230
+أستخدمه لعد خلايا ال red blood cells أول حاجة لازم
+
+178
+00:14:15,230 --> 00:14:20,140
+يكون anticoagulantيمنع تخسر الدم لإن إذا تخسر الدم
+
+179
+00:14:20,140 --> 00:14:25,060
+.. إذا تخسر الدم رح يصير في عندي clotting تجمع
+
+180
+00:14:25,060 --> 00:14:29,400
+للدم .. تجمع لل RBC عاملة زي clot زي كتلة و
+
+181
+00:14:29,400 --> 00:14:34,420
+بالتالي جلطة يعني فبالتالي هيك استهلاك عدد من
+
+182
+00:14:34,420 --> 00:14:38,460
+خلايا ال red blood cell صارلها trapping في ال clot
+
+183
+00:14:38,460 --> 00:14:43,180
+و يعني انحجزت في ال clot فبالتالي هيك رح يقلل عدد
+
+184
+00:14:43,180 --> 00:14:48,180
+خلايا الدم لل RBC ويعطيني error تمام؟لازم كمان
+
+185
+00:14:48,180 --> 00:14:51,800
+يكون antihemolysis يعني إيه إيش antihemolysis؟
+
+186
+00:14:51,800 --> 00:14:56,080
+يمنع تكسر الدم، يعني isotonic. Anti aggregation
+
+187
+00:14:56,080 --> 00:14:59,640
+يمنع تجمع خلاهي ال RBCs جنب بعضها البعض أو
+
+188
+00:14:59,640 --> 00:15:02,740
+تقاربها، لازم تكون هيك بعيدة عن بعضها البعض عشان
+
+189
+00:15:02,740 --> 00:15:08,550
+أنا أتمكن من عدهاAnti-relaw أيضًا ما يمنع تكدس ال
+
+190
+00:15:08,550 --> 00:15:12,210
+RBCs على بعضها البعض ويعرفنا إيش يعني كلمة relaw
+
+191
+00:15:12,210 --> 00:15:18,190
+يعني stacks like coins صح؟ أنه تلتثق ببعضها البعض
+
+192
+00:15:18,190 --> 00:15:22,030
+زي القطع المعدنية وغلط هذا طب أنا كده أعدها لازم
+
+193
+00:15:22,030 --> 00:15:26,370
+تكون منفصلة علشان أعدها لازم أيضًا من شروطه أنه
+
+194
+00:15:26,370 --> 00:15:31,870
+يكون ب reserve RBCs ship تمام؟ أنه يحافظ على شكل
+
+195
+00:15:31,870 --> 00:15:36,300
+ال RBCs اللي أتمكن من عدهافي عندي خيارات تمام
+
+196
+00:15:36,300 --> 00:15:40,860
+isotonic solution اللي هو isotonic saline و اتذكر
+
+197
+00:15:40,860 --> 00:15:43,980
+ان يعني ا .. ان احنا ذكرنا في محاضرة ال ESR ذكرنا
+
+198
+00:15:43,980 --> 00:15:47,200
+ال saline حكينا محلول ملحي المحلول الملحي المعقم
+
+199
+00:15:47,200 --> 00:15:51,380
+اللي انا بشتريه من الصيدلية لتعقيم جرح ل .. احيانا
+
+200
+00:15:51,380 --> 00:15:56,920
+برضه كمان بيحطوه ك .. ك .. في الوريد يعني كغداء او
+
+201
+00:15:56,920 --> 00:16:00,770
+يعني لحتى يرفع مثلا ..حجم الدم الناس اللي ضغطها
+
+202
+00:16:00,770 --> 00:16:03,870
+وعطي الناس اللي مغمة عليها كذا ممكن يحطولها إياها
+
+203
+00:16:03,870 --> 00:16:07,510
+ده محلول ملحي موجود فيه ملح الطعام Sodium Chloride
+
+204
+00:16:07,510 --> 00:16:12,930
+بس بنسبة معينة بنسبة 85% تمام؟ وبالتالي
+
+205
+00:16:14,890 --> 00:16:20,210
+كمية الأملح هذه مضبوطة على إنها تحافظ على الضغط
+
+206
+00:16:20,210 --> 00:16:23,690
+الأسموزي للخلايا فبالتالي هو Anti-Coagulant, Anti
+
+207
+00:16:23,690 --> 00:16:25,990
+-Hemolysis, Anti-Aggregation, Anti-Rouleau, We
+
+208
+00:16:25,990 --> 00:16:31,670
+preserve RBCs count أيضًا في عندي من المحليل
+
+209
+00:16:31,670 --> 00:16:36,290
+Hayam's solution تمام؟ Hayam's solution مكون من
+
+210
+00:16:36,290 --> 00:16:40,860
+Sodium Chlorideو ال sodium chloride عشان يحافظ على
+
+211
+00:16:40,860 --> 00:16:43,720
+ال osmotic pressure يعني عشان يكون isotonic تمام ب
+
+212
+00:16:43,720 --> 00:16:47,160
+provide isotonicity يعني عشان يخل المحلول isotonic
+
+213
+00:16:47,160 --> 00:16:51,580
+sodium sulfate طبعا وظيفة ال sodium sulfate أن هو
+
+214
+00:16:51,580 --> 00:16:56,380
+prevent roll off formation بيمنع ال roll off
+
+215
+00:16:56,380 --> 00:16:59,840
+formation تكون ال roll off formationmercuric
+
+216
+00:16:59,840 --> 00:17:03,320
+chloride اللي هو act as antiseptic اللي هو يعني زي
+
+217
+00:17:03,320 --> 00:17:08,720
+مادة متهرة او معاقمة تمنع نمو اي فطريات او عفن او
+
+218
+00:17:08,720 --> 00:17:12,860
+اي مايكروبات داخل هذا المحلول فبيضل نظيف بيضل خالي
+
+219
+00:17:12,860 --> 00:17:16,700
+من ال مايكروبات فمابيأثرش على القراءة انا وين ما
+
+220
+00:17:16,700 --> 00:17:22,480
+استخدمه بيضل نظيف ومايأثرلي على القراءةتمام؟ ذكرنا
+
+221
+00:17:22,480 --> 00:17:25,760
+احنا انه في محاضرة ال white pieces count انه لما
+
+222
+00:17:25,760 --> 00:17:30,040
+يكون في عندي في المحلول او على ال chamber او حتى
+
+223
+00:17:30,040 --> 00:17:34,000
+على العدسة في yeast خمائر او فطريات او عصن او اي
+
+224
+00:17:34,000 --> 00:17:38,930
+شيء ممكن يأثرلي على العد تمام؟فالmercuricucloride
+
+225
+00:17:38,930 --> 00:17:42,770
+act as antiseptic بيمنع نمو هذه الكائنات الدقيقة
+
+226
+00:17:42,770 --> 00:17:46,430
+اللي من شأنها تعيق عملية العد Distilled water أكيد
+
+227
+00:17:46,430 --> 00:17:50,770
+يعني المذيب في لهذه solutes كلها المذيب تبعها
+
+228
+00:17:50,770 --> 00:17:54,550
+distilled water تمام؟ إذا هذا هيام solution
+
+229
+00:17:54,550 --> 00:17:59,990
+isotonic بيمنع الرلوة وبرضه كمان antiseptic ويعني
+
+230
+00:18:01,520 --> 00:18:06,480
+سائل منيح لحتى نستخدمه ك diluent ل ال red blood
+
+231
+00:18:06,480 --> 00:18:10,300
+cell count أيضا في عندي gore solution موجود في
+
+232
+00:18:10,300 --> 00:18:13,760
+sodium sulfate صارنا بنعرف إيش يعني sodium sulfate
+
+233
+00:18:13,760 --> 00:18:16,780
+وضيفته ب prevention of low formation و ال glacial
+
+234
+00:18:16,780 --> 00:18:21,180
+acetic acid تمام و موجود أيضا distilled water
+
+235
+00:18:21,600 --> 00:18:25,620
+Citrate Formalin Solution النوع الرابع من المحاليل
+
+236
+00:18:25,620 --> 00:18:30,140
+مكوّن من الـ Trisodium Citrate و Formalin زي يعني
+
+237
+00:18:30,140 --> 00:18:34,860
+زي كأنه Buffer حتى يحافظ لي على الـRBCs تمام؟ أنتم
+
+238
+00:18:34,860 --> 00:18:38,540
+لاحظين هنا المكونات المحاليل مش محتاجين صبغلش لإن
+
+239
+00:18:38,540 --> 00:18:42,940
+الـRBCs أصلا يعني هي لونها Red فأنا لو جيت على ال
+
+240
+00:18:42,940 --> 00:18:46,220
+microscope شوفتها لو لعبت بالـFine Adjustment شوية
+
+241
+00:18:46,220 --> 00:18:49,080
+عشان أشوف ال details ممكن أشوف اللون الأحمر اللي
+
+242
+00:18:49,080 --> 00:18:53,220
+موجود داخل الخلايافسهل جدا أميزها لكن في ال white
+
+243
+00:18:53,220 --> 00:18:56,480
+pieces count كان ال solution لازم يكون فيه crystal
+
+244
+00:18:56,480 --> 00:19:00,160
+violet أو يعني صبغة معينة لحتى لما ألعب بال fine
+
+245
+00:19:00,160 --> 00:19:04,380
+adjustment أشوف أن ال white pieces مصبوغة عشان
+
+246
+00:19:04,380 --> 00:19:10,350
+أقدر أميزهاطيب بيحكيلي في بعض الحالات in certain
+
+247
+00:19:10,350 --> 00:19:15,150
+conditions Hayan solution may cause the clumping
+
+248
+00:19:15,150 --> 00:19:20,110
+of RBCs under law، تمام؟ و .. و .. و لكن يعني في
+
+249
+00:19:20,110 --> 00:19:24,550
+بعض الحالات ال Hayan solution ممكن يعملي clumping
+
+250
+00:19:24,550 --> 00:19:28,810
+لل RBCs، ممكن يعملي law formation، تجمعات لل RBCs،
+
+251
+00:19:28,810 --> 00:19:33,370
+فبالتالي إذا أنا صدفو أنا بستخدم Hayam و إجا عندي
+
+252
+00:19:33,370 --> 00:19:38,250
+patient و وجدت إنه عينته صار فيها clumping أو لورة
+
+253
+00:19:38,250 --> 00:19:41,250
+معناه هو هذا الشخص مش لازم أنا .. يعني هو ممكن
+
+254
+00:19:41,250 --> 00:19:46,250
+يكون حساس لمكونات Hayam solution فبالتالي مش لازم
+
+255
+00:19:46,250 --> 00:19:50,670
+أستخدم مع Hayam solution لازم أستخدمله للعد gore
+
+256
+00:19:50,670 --> 00:19:53,450
+solution تمام؟ Gore solution prevent these
+
+257
+00:19:53,450 --> 00:19:58,210
+problems بيمنع هذه المشاكلإذن في بعض الأحيان وليس
+
+258
+00:19:58,210 --> 00:20:04,030
+دوما تمام، gower solution يتفوق على هيام في منع ال
+
+259
+00:20:04,030 --> 00:20:07,470
+clumping و ال roll-off و هذا طبعا بيرجع لنوع
+
+260
+00:20:07,470 --> 00:20:12,240
+المريض يعني، ان دمه بيتأثر بمكونات هيام ولا لأ؟طيب
+
+261
+00:20:12,240 --> 00:20:16,120
+holy blood using edita or heparin as anticoagulant
+
+262
+00:20:16,120 --> 00:20:21,040
+طبعا انا عندى العينة تبعتي لازم يكون holy blood
+
+263
+00:20:21,040 --> 00:20:26,500
+بستخدم .. يكون طبعا ال tube edita او heparin طبعا
+
+264
+00:20:26,500 --> 00:20:30,670
+يكون فيها edita او heparinو ممكن كمان استخدم
+
+265
+00:20:30,670 --> 00:20:34,210
+capillary blood يعني من ال skin puncture باستخدام
+
+266
+00:20:34,210 --> 00:20:38,050
+الثومة بيبت يعني اعبي الثومة بيبت مباشرة طيب انا
+
+267
+00:20:38,050 --> 00:20:43,750
+ايش بلزمني؟ بلزمني بيبت تمام اما micro bibet او
+
+268
+00:20:43,750 --> 00:20:46,390
+اللي هي ال automatic bibet اللي انتوا بتشتغلوا
+
+269
+00:20:46,390 --> 00:20:50,350
+عليها في المختبرات او الثومة بيبت اللي مخصصة لل
+
+270
+00:20:50,350 --> 00:20:55,940
+RBCsتفتذكروا هاي الصورة اللي احنا ذكرنا .. عرضنا
+
+271
+00:20:55,940 --> 00:21:01,280
+فيها ثومة بيبت حكينا انه في عندي ثومة بيبت خاصة
+
+272
+00:21:01,280 --> 00:21:06,300
+بال white pieces count و ثومة بيبت خاصة بال R
+
+273
+00:21:06,300 --> 00:21:13,070
+pieces count تمام؟فإذا أنا بستخدم ال thoma pivot
+
+274
+00:21:13,070 --> 00:21:17,410
+اللي خاصة بال RBCs count حكينا حجمها أكبر شوية
+
+275
+00:21:17,410 --> 00:21:22,030
+لإنه نهاية التدريج عند 101 على خلاف ال YPCs نهاية
+
+276
+00:21:22,030 --> 00:21:25,790
+التدريج عند 11 طبعا أنا بملأها سواء كان من skin
+
+277
+00:21:25,790 --> 00:21:29,410
+puncture أو من ال tube اللي أنا عبتها يعني ال tube
+
+278
+00:21:29,410 --> 00:21:33,940
+فيها إيدي أو هبارين تمام؟ملقت فيها الدم اللي جمعته
+
+279
+00:21:33,940 --> 00:21:38,660
+من الوريد عملت mixing و بديت أعبي ال .. طبعا ال ..
+
+280
+00:21:38,660 --> 00:21:39,680
+ال .. ال .. ال .. ال .. ال .. ال .. ال .. ال .. ال
+
+281
+00:21:39,680 --> 00:21:39,800
+.. ال .. ال .. ال .. ال .. ال .. ال .. ال .. ال ..
+
+282
+00:21:39,800 --> 00:21:39,880
+ال .. ال .. ال .. ال .. ال .. ال .. ال .. ال .. ال
+
+283
+00:21:39,880 --> 00:21:40,580
+.. ال .. ال .. ال .. ال .. ال .. ال .. ال .. ال ..
+
+284
+00:21:40,580 --> 00:21:41,500
+ال .. ال .. ال .. ال .. ال .. ال .. ال .. ال .. ال
+
+285
+00:21:41,500 --> 00:21:41,840
+.. ال .. ال .. ال .. ال .. ال .. ال .. ال .. ال ..
+
+286
+00:21:41,840 --> 00:21:42,040
+ال .. ال .. ال .. ال .. ال .. ال .. ال .. ال .. ال
+
+287
+00:21:42,040 --> 00:21:42,480
+.. ال .. ال .. ال .. ال .. ال .. ال .. ال .. ال ..
+
+288
+00:21:42,480 --> 00:21:42,560
+ال .. ال .. ال .. ال .. ال .. ال .. ال .. ال .. ال
+
+289
+00:21:42,560 --> 00:21:44,940
+.. ال .. ال .. ال .. ال .. ال .. ال .. ال .. ال ..
+
+290
+00:21:44,940 --> 00:21:44,940
+ال .. ال .. ال .. ال .. ال .. ال .. ال .. ال .. ال
+
+291
+00:21:44,940 --> 00:21:50,760
+.. التمام مع أنها سميكة من برا شوي لكن لأ المسار
+
+292
+00:21:50,760 --> 00:21:54,160
+دقيق جدا و الدم بيطلع بخاصية ال capillary action
+
+293
+00:21:54,160 --> 00:21:58,800
+تمام بملأ الدم لعند النص و بعد هيك بمسح ال
+
+294
+00:21:58,800 --> 00:22:01,800
+capillary بنظف ال excess blood اللي عليها من برا
+
+295
+00:22:01,800 --> 00:22:05,600
+لإنه هيأثرلي على ال dilution إذا دل و بعد هيك إيش
+
+296
+00:22:05,600 --> 00:22:11,600
+بعمل بروح بملأ لحد ال 101 بال diluent طبعا ال
+
+297
+00:22:11,600 --> 00:22:14,560
+diluent اللي إحنا راح نستخدمه في المعمل هو ال
+
+298
+00:22:14,560 --> 00:22:22,990
+saline هوأول محلول ذكرناه ك daily one هو
+
+299
+00:22:22,990 --> 00:22:25,670
+ال isotonic saline اللي هو المحلول الملحي الطبيعي
+
+300
+00:22:25,670 --> 00:22:28,750
+يعني اللي .. اللي احنا ذكرناه متوفر وموجود في
+
+301
+00:22:28,750 --> 00:22:32,430
+الصيدليات بيتعقم نستخدمه كتير لل seal suspension
+
+302
+00:22:32,430 --> 00:22:38,170
+لحتى يعني أستخدم .. أعمل معلق خلايا فيه يعني سهل
+
+303
+00:22:38,170 --> 00:22:41,370
+ومتوفر سهل تحضيره أيضا
+
+304
+00:22:45,910 --> 00:22:46,410
+تمام
+
+305
+00:22:50,990 --> 00:22:55,710
+إذن أنا محتاجة bybit سواء كانت thermobibet المخصصة
+
+306
+00:22:55,710 --> 00:22:59,570
+لل RBCs أو حتى automatic bybit محتاجة ال chamber
+
+307
+00:22:59,570 --> 00:23:04,990
+تمام اللي هو يعني ال slide المخصصة لعد الخلايا و
+
+308
+00:23:04,990 --> 00:23:08,750
+ال .. شوفوا معايا ال .. ال .. ال chamber بيكون
+
+309
+00:23:08,750 --> 00:23:12,870
+مقسم لال .. ال .. ال .. يعني لمناطق على عد ال
+
+310
+00:23:12,870 --> 00:23:17,400
+white pieces ومناطق لعد ال RBCsهلأ اذا أحيانا في
+
+311
+00:23:17,400 --> 00:23:21,740
+بعض ال chambers تمام؟ في بعض ال chambers ما بيكون
+
+312
+00:23:21,740 --> 00:23:25,120
+.. هذه المنطقة بتكون ممسوحة يعني مافيش فيها ..
+
+313
+00:23:25,120 --> 00:23:28,980
+يعني مش مخصص لعبد RBCs بس ل white BCs فبسميه new
+
+314
+00:23:28,980 --> 00:23:34,280
+power chamber تمام؟إذا كان ال chamber موجود فيه
+
+315
+00:23:34,280 --> 00:23:38,600
+مناطق لعد ال RBCs بالإضافة لعد ال YBCs بسميه
+
+316
+00:23:38,600 --> 00:23:42,960
+improved neopower chamber، طبعا؟ يعني محسن، يعني
+
+317
+00:23:42,960 --> 00:23:48,360
+chamber مضافة عليه منطقة عد ال .. ال RBCsطمام؟
+
+318
+00:23:48,360 --> 00:23:52,820
+محتاجة ال light microscope، محتاجة جوز، شاش أو
+
+319
+00:23:52,820 --> 00:23:57,680
+cotton أو قطعة يعني قماشية لتنظيف إما ال .. ال ..
+
+320
+00:23:57,680 --> 00:24:02,040
+ال chamber لتنظيف ال pipe من برا، ثم ال pipe
+
+321
+00:24:02,040 --> 00:24:06,500
+لتنظيف عدسات ال microscope، إحنا محتاجين جوزطيب
+
+322
+00:24:06,500 --> 00:24:09,620
+انا بدى اعمل dilution احنا حكينا انا بعمل dilution
+
+323
+00:24:09,620 --> 00:24:13,540
+في ال thermobibet باخد لنص بعدين ل 101 خلينا نحسب
+
+324
+00:24:13,540 --> 00:24:18,280
+ال total dilution لهذه التجربة
+
+325
+00:24:18,280 --> 00:24:24,080
+انا طبعا حكينا باخد نص blood بعدين بكمل ل 101
+
+326
+00:24:24,080 --> 00:24:33,710
+تمام؟طيب و ال .. فبالتالي حكينا إنه الدم دايما
+
+327
+00:24:33,710 --> 00:24:39,290
+بيرجع أو بيستقر ب .. ال .. المستودع هنا يعني هذه
+
+328
+00:24:39,290 --> 00:24:41,710
+المنطقة فقط بيكون فيها ال diligent في ال white
+
+329
+00:24:41,710 --> 00:24:44,670
+pieces بيكون فيها فقط tech في ال RPCs هيكون فيها
+
+330
+00:24:44,670 --> 00:24:50,830
+فقط saline تمام؟ طيبو لما اجي انا اعبي ال chamber
+
+331
+00:24:50,830 --> 00:24:53,770
+بتخلص من المحتوى ال stem اللي هو هذا الواحد اللي
+
+332
+00:24:53,770 --> 00:24:57,510
+هنا اللي هو عبارة عن diluent فقط لحاله فإذا انا
+
+333
+00:24:57,510 --> 00:25:02,410
+بتخلص من ال .. ال واحد عبارة عن تلت قطرات او اربع
+
+334
+00:25:02,410 --> 00:25:05,410
+قطرات بنزلهم على ال tissue فبصير ال dilution نص
+
+335
+00:25:05,410 --> 00:25:14,850
+لمية تمام؟ يعني واحد إلى متين sorry واحد إلى متين
+
+336
+00:25:15,880 --> 00:25:19,980
+تمام فهذه هي الفكرة هذا ال dilution اللي انا بحضره
+
+337
+00:25:19,980 --> 00:25:22,760
+يعني واحد إلى مية او واحد ل متين واحد يعني هذا ال
+
+338
+00:25:22,760 --> 00:25:28,420
+total dilution فممكن انا احضره باستخدام بايبت
+
+339
+00:25:28,420 --> 00:25:32,720
+يوتيوب غير ال thomabibet اه عادي يعني شوفوا انا
+
+340
+00:25:32,720 --> 00:25:38,820
+بدي احضر واحد ل .. ل dilution طبعا اللي هو متين و
+
+341
+00:25:38,820 --> 00:25:45,870
+ال total متين واحد طيب جدش بدي احط لو حطيت هنالو
+
+342
+00:25:45,870 --> 00:25:53,810
+حطيت من ال blood عشرين microliter تمام؟ هيكون في
+
+343
+00:25:53,810 --> 00:26:00,830
+ال .. ال .. هيكون في ال tube .. اه و هستخدم
+
+344
+00:26:00,830 --> 00:26:07,750
+diluent عبارة عن ال volume تبعه4000 اللي هو عشرين
+
+345
+00:26:07,750 --> 00:26:11,310
+في متين طبعا اللي هو الضعف إذا انا استخدمت .. إذا
+
+346
+00:26:11,310 --> 00:26:14,090
+الواحد كان عبارة عن عشرين المتين مرة من العشرين
+
+347
+00:26:14,090 --> 00:26:19,210
+كام؟ 4000 طبعا يعني أربعة أمقال فإذا ممكن أنا أحضر
+
+348
+00:26:19,210 --> 00:26:22,190
+في تيوب أحط عشرين microliter من ال blood و أربعة
+
+349
+00:26:22,190 --> 00:26:27,090
+أمقال من ال diluting fluid اللي هو ال saline طبعا
+
+350
+00:26:27,090 --> 00:26:29,290
+طيب
+
+351
+00:26:30,820 --> 00:26:35,060
+إذا ممكن أحضره إما بطريقة ثومابيبت أسحب للنص أكمل
+
+352
+00:26:35,060 --> 00:26:39,840
+ل 101 أتخلص من أعمل mixing أتخلص من أول 3-4 قطرات
+
+353
+00:26:39,840 --> 00:26:44,120
+إذا ال dilution 1 ل 2 ك dilution أولي طبعا أنا
+
+354
+00:26:44,120 --> 00:26:48,180
+مابحكيش عن ال total dilution أنا بحكي نسبة ال
+
+355
+00:26:48,180 --> 00:26:53,300
+blood إلى ال diluent لما بقول 1 ل 2 تمام؟ أو إما
+
+356
+00:26:53,300 --> 00:26:58,180
+باستخدام ال micro pipette العادية باستخدام تيوب
+
+357
+00:26:58,180 --> 00:27:03,930
+باخد diluent و بحط عليه ال bloodطيب، لاحظوا هنا
+
+358
+00:27:03,930 --> 00:27:08,090
+أنا بحط أربع أمقال من ال saline، تمام؟ و بحط عليها
+
+359
+00:27:08,090 --> 00:27:11,210
+عشرين microliter من ال blood عشرين microliter كمية
+
+360
+00:27:11,210 --> 00:27:17,030
+قليلة جدا، تمام؟ فأنا لما ب .. ب .. بحاول أفضّيها،
+
+361
+00:27:17,030 --> 00:27:21,030
+بفضّيها في داخل المحلول و بعمل mixing منيح لحتى
+
+362
+00:27:21,030 --> 00:27:24,710
+أتأكد أن الطب و أنا طالعة تكون نظيفة و بطلع ضغطة
+
+363
+00:27:24,710 --> 00:27:27,750
+علشان ما أاخد volume معايا و أنا طالعة ما أؤثر على
+
+364
+00:27:27,750 --> 00:27:31,250
+ال dilution، تمام؟نفس الخطوات اللى اتبعناها
+
+365
+00:27:31,250 --> 00:27:35,650
+ودكرناها وشددنا عليها فى فحص ال homoglobin لما كنا
+
+366
+00:27:35,650 --> 00:27:39,030
+ناخد خمسة أمقال من ال drapkin اضيف عليها عشرين
+
+367
+00:27:39,030 --> 00:27:42,610
+microliter من ال blood وحكيتلكم انه بنفوت لجوا
+
+368
+00:27:42,610 --> 00:27:46,690
+المحلول بفضل عشرين microliter و بعمل mixing منيح
+
+369
+00:27:46,690 --> 00:27:49,790
+عشان تطلع التد نظيفة و بطلع دفتر عساس ما اخد
+
+370
+00:27:49,790 --> 00:27:54,810
+volumeتمام طيب بملق ال chamber هعلمكم ان شاء الله
+
+371
+00:27:54,810 --> 00:27:59,190
+بالمختبر كيف احنا بنعبي ال chamber بحط عليه cover
+
+372
+00:27:59,190 --> 00:28:02,230
+slip و المسافة الفاصلة اللي هي ال 1 من 10 ملي متر
+
+373
+00:28:02,230 --> 00:28:06,750
+مكعب هادي بنملقها في المحلول اللي احنا حضرناه و
+
+374
+00:28:06,750 --> 00:28:10,490
+بحط هذا ال hematocytometer على ال microscope و
+
+375
+00:28:10,490 --> 00:28:13,730
+ببدأ اجيب ال field طبعا انا بجيب ال field على
+
+376
+00:28:13,730 --> 00:28:17,440
+العدسة عشرةتمام؟ في ال white pieces بجيبه على
+
+377
+00:28:17,440 --> 00:28:21,220
+أربعة و بعدين بعيد على أشرة تمام؟ لكن هنا بجيبه
+
+378
+00:28:21,220 --> 00:28:24,920
+على عشرة و بعيد على أربعين طيب فبجيب ال field على
+
+379
+00:28:24,920 --> 00:28:29,840
+عشرة إيش بدي أشوف؟ خلونا مع بعض نكبر ال slide هنا
+
+380
+00:28:29,840 --> 00:28:35,720
+هاي أنا طبعا بدي أركز أخلي اللي تحت العدسة هو
+
+381
+00:28:35,720 --> 00:28:40,070
+المربع المركزي هذا اللي هو تقاطع المضغوطتمام؟ بحط
+
+382
+00:28:40,070 --> 00:28:44,770
+على تقاطع المضغوط تحت العدسة طيب التقاطع المضغوط
+
+383
+00:28:44,770 --> 00:28:47,870
+هذا أول ما أجيبه طبعا هو عبارة عن إيش؟ هو عبارة عن
+
+384
+00:28:47,870 --> 00:28:52,650
+خمسة وعشرين مربع صغير، خمسة في خمسة، هاي واحد،
+
+385
+00:28:52,650 --> 00:28:57,430
+اتنين، تلاتة، أربعة، خمسةواحد اتنين تلاتة اربعة
+
+386
+00:28:57,430 --> 00:29:03,430
+خمسة خمسة خمسة خمسة وعشرين تمام؟ طيب ايش المربعات
+
+387
+00:29:03,430 --> 00:29:06,370
+اللي انا ب .. طبعا كل مربع من هدول الخمسة وعشرين
+
+388
+00:29:06,370 --> 00:29:11,970
+مقسم إلى ستاشر مربع يعني مقسم إلى ستاشر زي هنا
+
+389
+00:29:11,970 --> 00:29:16,050
+طيب؟ فعادي يعني لما انا اكبره على اربعين بيكبر
+
+390
+00:29:16,050 --> 00:29:19,250
+بيصير زي هيك بيصير عبارة عن ستاشر مربع كبار في بعض
+
+391
+00:29:19,250 --> 00:29:25,190
+طيب؟فالمربعات اللي أنا بستخدمها علشان أعد عليها هي
+
+392
+00:29:25,190 --> 00:29:29,230
+الأربع اللي على الأطراف و اللي في المركز تمام؟ إذا
+
+393
+00:29:29,230 --> 00:29:35,790
+أول إشي بحط ال chamber على العدسة
+
+394
+00:29:35,790 --> 00:29:41,040
+عشرة بجيب ال field تمام؟بجيب ال field بحيث انه
+
+395
+00:29:41,040 --> 00:29:44,240
+تكون منطق التقاطع المضغوط هي اللي تحت ال
+
+396
+00:29:44,240 --> 00:29:48,520
+microscope او تحت العدسة و بعدين بركب .. ب .. بكبر
+
+397
+00:29:48,520 --> 00:29:53,020
+طبعا على العدسة 40 فبي .. ب .. بخلي ان العدسة
+
+398
+00:29:53,020 --> 00:29:56,780
+تتوجه على احد المربعات هدول هذا المربع الكبير اللي
+
+399
+00:29:56,780 --> 00:30:00,860
+هو تقاطع المضغوط هو عبارة عن 25 مربع كل واحد من ال
+
+400
+00:30:00,860 --> 00:30:07,890
+25 مقسم إلى 16 مربع صغار يعني زي هدولتمام؟ طيب ف
+
+401
+00:30:07,890 --> 00:30:12,230
+.. ب .. ببدأ أعد فيهم طبعا ب .. ببدأ أعد كيف أعد
+
+402
+00:30:12,230 --> 00:30:16,510
+.. كيف بعرف حالي إن أنا في المربع الأول أو التاني
+
+403
+00:30:16,510 --> 00:30:19,730
+أو التالت أو كذا شوفوا معايا ب .. من خلال
+
+404
+00:30:19,730 --> 00:30:23,150
+المستطيلات هذه اللي على الجانب تمام؟ ب .. شوف ..
+
+405
+00:30:23,150 --> 00:30:26,130
+إذا كان في مستطيلات هنا على الجانب و هنا معناها
+
+406
+00:30:26,130 --> 00:30:30,310
+أنا في الأول إذا كانت المستطيلات هيك و هيك معناها
+
+407
+00:30:30,310 --> 00:30:35,340
+أنا في اللي .. اللي على الطرف التاني رقم اتنينهنا
+
+408
+00:30:35,340 --> 00:30:39,960
+رقم تلاتة تمام المستطيلات بهذا الشكل هنا أربعة و
+
+409
+00:30:39,960 --> 00:30:43,660
+هذا اللي في المركز بيكون طبعا كل مربع له حدود تلت
+
+410
+00:30:43,660 --> 00:30:47,260
+خطوط هك و تلت خطوط هك تمام هم مش مباينين بيكونوا
+
+411
+00:30:47,260 --> 00:30:51,660
+في ال .. في الصورة هنا لكن على ال .. ال microscope
+
+412
+00:30:51,660 --> 00:30:54,580
+هتشوفوهم فانت طبعا كيف بديك تمشي لما تصل إلى
+
+413
+00:30:54,580 --> 00:30:59,000
+المركز اما بمشي تلت خطوات لتحت يعني هي واحد اتنين
+
+414
+00:30:59,000 --> 00:31:04,040
+تلاتة واحد اتنين تلاتة اذا انا في المركزتمام؟ هيك
+
+415
+00:31:04,040 --> 00:31:08,000
+بيكون ال .. او يعني انه انت في المربع اترك اتنين
+
+416
+00:31:08,000 --> 00:31:12,700
+بعدين كمان اترك امشي اتنين بتلاقي حالك في المركز
+
+417
+00:31:12,700 --> 00:31:17,780
+هنتعلم هذه كلها خطوة بخطوة كيف احنا بنتحرك تمام؟
+
+418
+00:31:17,780 --> 00:31:21,480
+طيب بالزمني اعرف انه هذا المربع الكبير اللي بيحتوي
+
+419
+00:31:21,480 --> 00:31:25,400
+على خمسة و عشرين مربع صغير هو نفس أبعد هذا يعني
+
+420
+00:31:25,400 --> 00:31:31,060
+هذا واحدو هذا واحد millimeter تمام طب هذا الصغير
+
+421
+00:31:31,060 --> 00:31:35,880
+طب ما هو إذا هذا واحد و مقسم إلى خمس أجزاء تمام
+
+422
+00:31:35,880 --> 00:31:40,340
+خمس مربعات معناها البعد المربع الصغير اللي هو
+
+423
+00:31:40,340 --> 00:31:45,450
+عبارة عن اتنين من عشرة اتنين من عشرة تماممهمين
+
+424
+00:31:45,450 --> 00:31:51,350
+للحسابات، لذا هاي هنا تقاطع المضغوط، بعدين بجيبه
+
+425
+00:31:51,350 --> 00:31:55,210
+على العدسة عشرة، بحول على أربعين، بحدد أي مربع أنا
+
+426
+00:31:55,210 --> 00:31:58,710
+فيه، ببدأ أعد، بعدين بنتقل إلى المربعات الأخرى، و
+
+427
+00:31:58,710 --> 00:32:03,450
+بعدها، تمام؟ و عارفنا كيف بحدد حالي وين أنا في أي
+
+428
+00:32:03,450 --> 00:32:07,890
+مربع، بعد ما أخلص عد، بدي أجي أعمل الحساباتطبعا ال
+
+429
+00:32:07,890 --> 00:32:12,210
+total RPCs count تبعي نفس قانون ال total YPCs
+
+430
+00:32:12,210 --> 00:32:16,110
+count اللي هو عبارة عن N ولكن هنا N هو مجموع
+
+431
+00:32:16,110 --> 00:32:20,270
+الخلايا في خمس مربعات، تمام؟ يعني إذا عديت في
+
+432
+00:32:20,270 --> 00:32:26,870
+منطقة واحدة فقط بجمع الخمس مربعات و بعود في N إذا
+
+433
+00:32:26,870 --> 00:32:30,110
+عديت في المنطقة تانية من الات shape لازم أقسم ع
+
+434
+00:32:30,110 --> 00:32:33,250
+اتنين لإن بكون عديت عشر مربعات، خمسة فوق وخمسة تحت
+
+435
+00:32:33,680 --> 00:32:38,280
+تمام؟ فبقى أقسم على اتنين ضرب ال dilution factor
+
+436
+00:32:38,280 --> 00:32:43,760
+طبعا احنا حكينا ان ال dilution واحد إلى متين تمام؟
+
+437
+00:32:43,760 --> 00:32:49,880
+فال .. ال .. ال dilution factor متين تمام؟ انا
+
+438
+00:32:49,880 --> 00:32:54,900
+بفضل يا بنات ان هذه ال .. ال .. المعلومة بس
+
+439
+00:32:54,900 --> 00:32:59,120
+اتعدلوها و اتخلوها .. اتخلوها متين هيك لإنه طبعا
+
+440
+00:32:59,120 --> 00:33:03,790
+هي .. هي الأفضل هي الأدق هيك لتكون متينطيب ف .. ك
+
+441
+00:33:03,790 --> 00:33:07,990
+.. ك total يعني لإنه أنا باخد الحجم نص و بكمل لل
+
+442
+00:33:07,990 --> 00:33:12,190
+.. للمية و واحد يعني النص أصلا عبارة .. يعني لما
+
+443
+00:33:12,190 --> 00:33:17,390
+أجيب .. لما أفضي واحد كمان النص و ال .. ال .. ال
+
+444
+00:33:17,390 --> 00:33:20,810
+.. المية ما هو النص محتل جزء من المية تمام؟ يعني
+
+445
+00:33:20,810 --> 00:33:25,050
+ال total هي المية تمام؟ فبده .. لما بدي أحاول
+
+446
+00:33:25,050 --> 00:33:27,910
+أتخلص من النص هذا يعني أخليه واحد بده يصير هذه
+
+447
+00:33:27,910 --> 00:33:28,390
+متين
+
+448
+00:33:33,940 --> 00:33:38,400
+تمام في ال dilution factor اللي هو 200 و ال volume
+
+449
+00:33:38,400 --> 00:33:42,160
+correction factor طب احنا بنعد per microliter واحد
+
+450
+00:33:42,160 --> 00:33:46,100
+طب انا هلأ عديت الحجم لازم يكون microliter واحد
+
+451
+00:33:46,100 --> 00:33:49,600
+لإن ال .. ال .. الوحدة اللي بدي استخدمها هي عبارة
+
+452
+00:33:49,600 --> 00:33:58,020
+عن سل per microliter تمام؟ سل per
+
+453
+00:33:58,020 --> 00:34:05,250
+microliter تمام؟طيب ف .. بدي بال microliter الواحد
+
+454
+00:34:05,250 --> 00:34:08,630
+كام بيكون خلايا فبدأ أعمل بحاجة اسمها volume
+
+455
+00:34:08,630 --> 00:34:12,050
+correction و أجيبكوا volume correction factor طب
+
+456
+00:34:12,050 --> 00:34:15,010
+نحسب الحجم نفس ما كنا نعمل في ال white pieces بدي
+
+457
+00:34:15,010 --> 00:34:19,090
+أحسب الحجم اللي انعدت فيه الخلايا احنا حكينا ان
+
+458
+00:34:19,090 --> 00:34:24,440
+المربع هذا كل هذانفس الكبير هذا هذا حكينا في ال
+
+459
+00:34:24,440 --> 00:34:26,840
+white pieces كام حجم وكنا نعد فيه white pieces هذا
+
+460
+00:34:26,840 --> 00:34:32,460
+واحد في واحد تمام؟ أبعاده فهذا بيكون واحد في واحد
+
+461
+00:34:32,460 --> 00:34:36,940
+زيه طب أنا بعد في هذا الصغير اللي هو عبارة عن خمس
+
+462
+00:34:36,940 --> 00:34:42,220
+المسافة أبعاده صح؟ هي واحد اتنين تلاتة أربعة خمسة
+
+463
+00:34:42,220 --> 00:34:46,260
+إذا هذا البعد هو عبارة عن خمس المسافة كلها إذا
+
+464
+00:34:46,260 --> 00:34:50,670
+المسافة كلها واحد خمسها كم رح يكون؟الخمس يعني
+
+465
+00:34:50,670 --> 00:34:55,230
+اتنين من عشرة .. اتنين من عشرة في اتنين من عشرة
+
+466
+00:34:55,230 --> 00:34:59,950
+تمام في ال point واحد اللي هو المسافة ما بين ال
+
+467
+00:34:59,950 --> 00:35:04,870
+chamber نفسه و ال cover slip اللي هي الارتفاع تبع
+
+468
+00:35:04,870 --> 00:35:07,910
+العينة او الارتفاع اللي موجود .. اللي موجودة فيه
+
+469
+00:35:07,910 --> 00:35:10,490
+العينة اللي هو المسافة ما بين ال chamber و ال
+
+470
+00:35:10,490 --> 00:35:14,900
+cover slip واحد من عشرة حكينا .. ذكرناهاتمام؟ فإذا
+
+471
+00:35:14,900 --> 00:35:19,640
+بدي يكون الحجم فاصلة اتنين ضرب فاصلة اتنين ضرب
+
+472
+00:35:19,640 --> 00:35:24,960
+فاصلة واحد طول عرض ارتفاع فبدي يكون هذا هو الحجم
+
+473
+00:35:24,960 --> 00:35:28,200
+تمام؟ أنا بعيد في خمس مربعات إذا الحجم الكل اللي
+
+474
+00:35:28,200 --> 00:35:33,200
+عديت فيه خمسة في هذا فهيطلع ليإتنين من مية تمام؟
+
+475
+00:35:33,200 --> 00:35:36,700
+إتنين من مية لما أعمل .. أجيب factor يعني مقلوبها
+
+476
+00:35:36,700 --> 00:35:41,180
+اللي هو عبارة عن خمسين فإذا اللي هو عبارة عن ال ..
+
+477
+00:35:41,180 --> 00:35:46,960
+ال .. القانون ال N عدد المجموع الخلايا في خمس
+
+478
+00:35:46,960 --> 00:35:55,020
+مربعات ضرب متين ضرب خمسين يعني N في عشر تلاف بطل
+
+479
+00:35:55,020 --> 00:35:59,740
+عدد ال Rbcsطبعا in certain conditions such as
+
+480
+00:35:59,740 --> 00:36:02,540
+polycythemia, red blood cell count may be
+
+481
+00:36:02,540 --> 00:36:05,500
+extremely high في بعض الحالات زي ال polycythemia
+
+482
+00:36:05,500 --> 00:36:09,980
+ال RBCs بتكون عالية جدا، إيش بدي أعمل؟ والله هذه
+
+483
+00:36:09,980 --> 00:36:13,500
+المعلومة ذكرنا إشي مشابه لإلها في ال YBCs count
+
+484
+00:36:13,620 --> 00:36:16,020
+حكينا في ال white pieces count لما يكون عندي
+
+485
+00:36:16,020 --> 00:36:20,620
+leukocytosis يعني ارتفاع في ال white pieces بروح
+
+486
+00:36:20,620 --> 00:36:25,260
+بزود ال dilution تمام؟ و هنا أيضا كمان بدي ازود
+
+487
+00:36:25,260 --> 00:36:28,780
+نفس الاشي بدي ازود ال dilution ففي حالات ال
+
+488
+00:36:28,780 --> 00:36:32,000
+polycythemia اللي فيها ارتفاع في ال red blood cell
+
+489
+00:36:32,000 --> 00:36:36,780
+بحكيلي انه زودي ال dilution يعني ده اخففي اكتر
+
+490
+00:36:36,780 --> 00:36:40,320
+اعملي larger dilution يعني بدل ما اعمل 1 ل 200
+
+491
+00:36:40,320 --> 00:36:46,260
+خليني اعمل 1 ل 300 تمام؟خليني أعمل واحد لتلت مية
+
+492
+00:36:46,260 --> 00:36:51,060
+يعني بدي أضيف عشرين microliter لست أمقال ال blood
+
+493
+00:36:51,060 --> 00:36:55,960
+طيب و الشخص
+
+494
+00:36:55,960 --> 00:37:03,140
+اللي عنده .. الشخص اللي عنده anemia تمامالشخص اللي
+
+495
+00:37:03,140 --> 00:37:08,220
+عنده anemia يعني low RBC count بقلل ال dilution
+
+496
+00:37:08,220 --> 00:37:11,600
+بعمل smaller dilution يعني بدل ما هو 1 ل 200 خلينا
+
+497
+00:37:11,600 --> 00:37:17,690
+نعمل 1 ل 100 يعني 20 microliter على 2 ml في tubeمش
+
+498
+00:37:17,690 --> 00:37:22,370
+في الصومة بايبت make certain تكوني متأكدة انه ال
+
+499
+00:37:22,370 --> 00:37:25,610
+بايبت ال hematocytometer and the covering glass
+
+500
+00:37:25,610 --> 00:37:29,350
+are free from their cleaned and dried blood لازم
+
+501
+00:37:29,350 --> 00:37:32,510
+الأدوات اللي بنستخدمها سواء كانت بايبت او
+
+502
+00:37:32,510 --> 00:37:35,250
+hematocytometer او حتى ال cover slip ال covering
+
+503
+00:37:35,250 --> 00:37:41,870
+glassلازم يكون خالي من الغبرة، أي أشياء بتعمل
+
+504
+00:37:41,870 --> 00:37:46,830
+اتساخ، بتلتسق عليه، بتعمل يعني إعاقة للرؤية و
+
+505
+00:37:46,830 --> 00:37:50,970
+للعدد و أيضا الدم الجاف ensure that the diluting
+
+506
+00:37:50,970 --> 00:37:54,670
+fluid is free from blood، كمان ال diluting fluid
+
+507
+00:37:54,670 --> 00:37:58,190
+اللي أنا بدى أستخدمه لازم يكون نظيف، مافيش فيه دم
+
+508
+00:37:58,190 --> 00:38:03,250
+تمام أو أي contamination أخرىو ال RPCs takes
+
+509
+00:38:03,250 --> 00:38:07,910
+longer to perform than YPCs طبعا عد ال RPCs بيكون
+
+510
+00:38:07,910 --> 00:38:11,910
+بياخد وقت أطول لإن ان انت بتعدي في خمس مربعات و
+
+511
+00:38:11,910 --> 00:38:15,250
+بديك فترة لما تنتقل من مربع لآخر و أصلا تجيب
+
+512
+00:38:15,250 --> 00:38:19,410
+المربع الأساسي اللي هو تقاطع المضغوط فيعني بديك
+
+513
+00:38:19,410 --> 00:38:24,930
+فترة ل .. تتمرني عليه فهو بياخد وقت أطول ال RPCs
+
+514
+00:38:24,930 --> 00:38:29,840
+countفي .. ك .. كعمل يعني كمدة عمل فبالتالي بيحكي
+
+515
+00:38:29,840 --> 00:38:31,320
+لي إنه لازم
+
+516
+00:38:34,000 --> 00:38:40,040
+لازم انت تتأكدي انه ال .. ال .. ال chamber ما جاف
+
+517
+00:38:40,040 --> 00:38:45,040
+تمام؟ لإنه جفاف ال chamber هي .. هيأثرلي على العدل
+
+518
+00:38:45,040 --> 00:38:48,020
+لازم انا ساعتها اشتغل proceed as quickly as
+
+519
+00:38:48,020 --> 00:38:51,840
+possible تمام؟ و ايضا احد العوامل اللي بيخلي انه
+
+520
+00:38:51,840 --> 00:38:56,060
+عملية ال RBCs count تاخد وقت أطول انه اصلا انت
+
+521
+00:38:56,060 --> 00:39:00,880
+بتعدي عدد خلايا اكبريعني هتشوفوا ال RPC ال white
+
+522
+00:39:00,880 --> 00:39:05,000
+PCs يعني يلّا .. يلّا يلّا تشوفي خلايا في المربعات
+
+523
+00:39:05,000 --> 00:39:08,080
+لكن في ال RPC بتشوفي عدد كبير من ال .. من الخلايا
+
+524
+00:39:08,080 --> 00:39:13,040
+و لازم تعديها فلإن عددها أكبر بتاخد وقت أطول أيضًا
+
+525
+00:39:13,040 --> 00:39:16,140
+في العد the range of error نسبة الخطأ في هذه
+
+526
+00:39:16,140 --> 00:39:18,660
+الطريقة في هذه التجربة مقارنتها بال .. بال
+
+527
+00:39:18,660 --> 00:39:24,500
+automatic يعني بال .. بالأجهزة من 10 إلى 20% نسبة
+
+528
+00:39:24,500 --> 00:39:29,360
+خطأهيك بنكون انتهينا من محاضرتنا لليوم طبعا بس بدي
+
+529
+00:39:29,360 --> 00:39:34,240
+أذكرلكم معلومة احنا ما ذكرناها بال white pieces
+
+530
+00:39:34,240 --> 00:39:36,120
+count حكينا انه
+
+531
+00:39:39,360 --> 00:39:43,980
+هنا ال .. ال R .. هذا ال normal range ل ال .. ال
+
+532
+00:39:43,980 --> 00:39:46,940
+.. ال RPCs count وحكينا عددها بالملايين عشر أقل
+
+533
+00:39:46,940 --> 00:39:50,080
+ستة سل بين microliter الوحدة سل بين microliter
+
+534
+00:39:50,080 --> 00:39:56,160
+تمام ال white PCs عددها بالألف تمام و هي من أربعة
+
+535
+00:39:56,160 --> 00:40:01,360
+و نص ألف لحداشر ألفsilver microliter طبعا لما بدي
+
+536
+00:40:01,360 --> 00:40:06,320
+احول ل liter بدي اضرب في عشرة او استسعى بس لو per
+
+537
+00:40:06,320 --> 00:40:17,360
+microliter بدها تكون زي هيك من اربعة و نص بدها
+
+538
+00:40:17,360 --> 00:40:24,360
+تكون هيك تمام ضرب عشرة
+
+539
+00:40:26,020 --> 00:40:30,520
+هي العشرة أس تلاتة
+
+540
+00:40:30,520 --> 00:40:38,900
+تمام هدي أس تلاتة مافيش عندي طريقة أرفعها مش مهم
+
+541
+00:40:38,900 --> 00:40:42,020
+هي هدي أس يعني power تمام
+
+542
+00:40:49,340 --> 00:40:53,100
+تمام انتوا افهموها انها هي هدي أصبور عشرة أص تلاتة
+
+543
+00:40:53,100 --> 00:41:00,640
+طبعا ايش ال .. هي .. هي كبير microliter تمام؟ هاي
+
+544
+00:41:00,640 --> 00:41:04,680
+ال microliter طبعا يعني انا ب .. بكتبها تجاوزا أم
+
+545
+00:41:04,680 --> 00:41:06,700
+ال .. sorry
+
+546
+00:41:09,010 --> 00:41:14,010
+ب .. بكتوبها تجاوزا هي اللي زي ال .. ال capital M
+
+547
+00:41:14,010 --> 00:41:18,470
+و ال L لكن هي يعني microliter بتنكتب بشكل .. بشكل
+
+548
+00:41:18,470 --> 00:41:23,310
+مختلف اللي هي هيك، تمام؟ أو ممكن أحكي زي .. يعني
+
+549
+00:41:23,310 --> 00:41:28,690
+أخد و أحكي سلper microliter يعني نفس ال .. الوحدة
+
+550
+00:41:28,690 --> 00:41:32,730
+يعني سواء كتبت العدد و قولت per microliter او حكيت
+
+551
+00:41:32,730 --> 00:41:36,210
+silver microliter نفس المعنى تمام؟ اذا هذا هو ال
+
+552
+00:41:36,210 --> 00:41:40,430
+range من اربعة و نص لحداشر الف او من اي يعني اربعة
+
+553
+00:41:40,430 --> 00:41:43,170
+تلاف و نص لحداشر الف و في ناس بتحكي من تلت تلاف
+
+554
+00:41:43,170 --> 00:41:48,030
+لحداشر الفطبعا و Jadir بتذكر أن هذه ال normal
+
+555
+00:41:48,030 --> 00:41:52,750
+ranges بتختلف طبعا من فئة إلى أخرى من ناس من
+
+556
+00:41:52,750 --> 00:41:56,650
+population ل population من مختبر ل مختبر فبالتالي
+
+557
+00:41:56,650 --> 00:41:59,610
+احنا مابنحفظ normal ranges ولكن على الأقل بنكون
+
+558
+00:41:59,610 --> 00:42:04,280
+يعني ..مدركينها يعني okay طبعا أنا مش .. مش طالبة
+
+559
+00:42:04,280 --> 00:42:07,200
+منك تحفظي بال .. بالضبط بالضبط ال normal ranges بس
+
+560
+00:42:07,200 --> 00:42:11,340
+على الأقل تكوني عارفة يعني أشياء في .. بنسميها
+
+561
+00:42:11,340 --> 00:42:14,880
+headlines يعني أنه مثلا والله ال homoglobin عند ال
+
+562
+00:42:14,880 --> 00:42:20,160
+female من ال 11 ل 13 أو من 11 ل 14 من 3000 ل 11000
+
+563
+00:42:20,160 --> 00:42:25,560
+أو من 4000 ل .. ل 11000 ال YPCs ما بين الأربعة ل
+
+564
+00:42:25,560 --> 00:42:30,820
+.. ل الخمسة و نص ال .. ال .. ال Y .. ال RPCs وها
+
+565
+00:42:30,820 --> 00:42:31,120
+كذا
+
+566
+00:42:34,850 --> 00:42:39,770
+تمام؟ هيك بنكون وصلنا لمحاضرتنا .. لنهاية محاضرتنا
+
+567
+00:42:39,770 --> 00:42:43,690
+لليوم أتمنى إنها تكون بسيطة عليكم يعطيكم ألف عافية
+
diff --git a/PL9fwy3NUQKwY9jl8xw-bWkVZkWcGGyspd/FgXeSCpsx3M.srt b/PL9fwy3NUQKwY9jl8xw-bWkVZkWcGGyspd/FgXeSCpsx3M.srt
new file mode 100644
index 0000000000000000000000000000000000000000..13dfb5df698fbabbeea9fc268bebd1f13f9d538b
--- /dev/null
+++ b/PL9fwy3NUQKwY9jl8xw-bWkVZkWcGGyspd/FgXeSCpsx3M.srt
@@ -0,0 +1,1517 @@
+1
+00:00:06,410 --> 00:00:10,510
+السلام عليكم ورحمة الله وبركاته طالبات العزيزات،
+
+2
+00:00:10,510 --> 00:00:14,410
+إن شاء الله اليوم راح نبلش محاضرة جديدة بعنوان
+
+3
+00:00:14,410 --> 00:00:18,990
+centrifuges، أي أجهزة الطرد المركزي أو جهاز
+
+4
+00:00:18,990 --> 00:00:25,310
+الطرد المركزي، تمام؟ طيب، بدايةً خلونا نحكي عن
+
+5
+00:00:25,310 --> 00:00:30,350
+مكونات الدم blood components، تمام؟ لما حكينا إنه عينة
+
+6
+00:00:30,350 --> 00:00:34,690
+الدم إذا أنا فصلتها باستخدام جهاز الـ centrifuge
+
+7
+00:00:34,690 --> 00:00:39,270
+باستخدام جهاز الطرد المركزي، الـ RBCs خلايا
+
+8
+00:00:39,270 --> 00:00:42,210
+ثقيلة الوزن راح تكون موجودة في الـ sediment في
+
+9
+00:00:42,210 --> 00:00:47,610
+القاع، تمام، at the bottom، ويليها طبقة من الـ
+
+10
+00:00:47,610 --> 00:00:51,290
+الـ buffy coat التي تحتوي على الـ white BCs والـ
+
+11
+00:00:51,290 --> 00:00:55,510
+platelets خلايا الدم البيضاء والصفائح الدموية، و
+
+12
+00:00:55,510 --> 00:00:59,350
+راح يكون بعدها البلازما التي هي أخف وزناً، و
+
+13
+00:00:59,350 --> 00:01:04,910
+تكون at the top، تمام. الـ RBCs راح تحتل نسبة خمسة وأربعين
+
+14
+00:01:04,910 --> 00:01:09,710
+بالمائة، والـ buffy coat أقل من
+
+15
+00:01:09,710 --> 00:01:15,010
+واحد بالمائة، والـ plasma خمسة وخمسون
+
+16
+00:01:15,010 --> 00:01:21,450
+بالمائة، تمام؟ طبعاً في مراجع يعني ممكن تكون تذكر
+
+17
+00:01:21,450 --> 00:01:25,450
+أن الـ RBCs تحتل نسبة أربعة وأربعين بالمائة، والـ
+
+18
+00:01:25,450 --> 00:01:29,070
+buffy coat واحد بالمائة، والـ plasma خمسة وخمسون
+
+19
+00:01:29,070 --> 00:01:33,400
+بالمائة، يعني مجموعهم مئة بالمائة. هلأ لو كانت
+
+20
+00:01:33,400 --> 00:01:36,420
+لو أنا حكيت لك الـ RBCs خمسة وأربعون
+
+21
+00:01:36,420 --> 00:01:38,820
+بالمائة أو أربعة وأربعون بالمائة ما في خلاف
+
+22
+00:01:38,820 --> 00:01:43,060
+طبعاً؟ لو كنت… لو حكيت أنه الـ RBCs خمسة وخمسون
+
+23
+00:01:43,060 --> 00:01:46,980
+بالمائة، بكون بعتبر أنه الـ buffy coat
+
+24
+00:01:46,980 --> 00:01:50,600
+negligible، يعني مهمل. أما إذا حكيت لك أنه أربعة وأربعون
+
+25
+00:01:50,600 --> 00:01:52,920
+بالمائة، بكون معتبرة أن الـ buffy coat واحد
+
+26
+00:01:52,920 --> 00:01:57,710
+بالمائة، طبعاً؟ طيب، الفكرة أنه باستخدام جهاز الطرد
+
+27
+00:01:57,710 --> 00:02:02,250
+المركزي، يتم فصل أجزاء الـ blood أو عينة الدم إلى
+
+28
+00:02:02,250 --> 00:02:07,130
+طبقة من الخلايا الثقيلة موجودة في القاع، يليها طبقة
+
+29
+00:02:07,130 --> 00:02:11,830
+أخف وزناً، الموجودة وهي الـ buffy coat، ثم الطبقة
+
+30
+00:02:11,830 --> 00:02:15,670
+الأخف وزناً على الإطلاق، أو أخف واحدة فيهم، حتكون موجودة
+
+31
+00:02:15,670 --> 00:02:21,670
+at the top. إذن هذا ما يفعله الـ centrifuge، يحدث بفعل
+
+32
+00:02:21,670 --> 00:02:25,850
+الـ centrifuge، باستخدام جهاز الطرد المركزي أو جهاز
+
+33
+00:02:25,850 --> 00:02:30,150
+الـ centrifuge الذي نستخدمه في المختبرات. centrifuge
+
+34
+00:02:30,150 --> 00:02:34,990
+شو يعني centrifuge؟ هو عبارة عن equipment، it's
+
+35
+00:02:34,990 --> 00:02:38,870
+equipment that is used to separate solid matter
+
+36
+00:02:38,870 --> 00:02:42,450
+from liquid suspension by means of centrifugal
+
+37
+00:02:42,450 --> 00:02:46,790
+forces. أنا بفصل الأجزاء الصلبة أو الأجزاء الثقيلة
+
+38
+00:02:46,790 --> 00:02:51,770
+المعلقة داخل سائل بفعل أو بتأثير أو بأثر الـ
+
+39
+00:02:51,770 --> 00:02:56,190
+centrifugal forces، تمام؟ نفس الفكرة بالنسبة للـ
+
+40
+00:02:56,190 --> 00:02:59,750
+blood، الـ blood هو عبارة عن خلايا، عن أجزاء صلبة،
+
+41
+00:02:59,750 --> 00:03:03,950
+خلايا صلبة، تمام؟ معلقة في الـ plasma التي هي السائل
+
+42
+00:03:03,950 --> 00:03:08,680
+تمام؟ إذن solid matter هو الخلايا، from يعني أنا بدي
+
+43
+00:03:08,680 --> 00:03:12,880
+أفصلها from liquid suspension من السائل الذي…
+
+44
+00:03:12,880 --> 00:03:16,100
+الذي… الذي معلقة فيه، الذي هو عبارة عن الـ plasma
+
+45
+00:03:16,100 --> 00:03:20,420
+بتأثير الـ centrifugal forces، الـ sediment أو الـ
+
+46
+00:03:20,420 --> 00:03:27,250
+solid matter، ممكن يكون خلايا، ممكن يكون بكتريا، cast
+
+47
+00:03:27,250 --> 00:03:32,690
+رواسب، ممكن يكون parasites، طفيليات، ويكون معلق
+
+48
+00:03:32,690 --> 00:03:37,510
+suspended in a fluid، معلق في سائل. لما أنا بأثر
+
+49
+00:03:37,510 --> 00:03:42,570
+عليه بقوة الـ centrifugal forces، بقوة الطرد المركزي،
+
+50
+00:03:42,570 --> 00:03:47,610
+أنا ساعتها بفصله، بحيث أن هذا الـ sediment بيترسب،
+
+51
+00:03:47,610 --> 00:03:52,490
+هذا الـ solid matter بيترسب في قاع الـ tube، والسائل
+
+52
+00:03:52,490 --> 00:03:57,070
+بيكون موجود على السطح، بينتقل إلى السطح،
+
+53
+00:03:57,070 --> 00:04:01,710
+تمام؟ هذه centrifugal forces هي عبارة عن force
+
+54
+00:04:01,710 --> 00:04:07,850
+greater than that of gravity، هي أضعاف قوة الجاذبية
+
+55
+00:04:07,850 --> 00:04:11,560
+الأرضية، هي قوة الطرد المركزي. the suspended
+
+56
+00:04:11,560 --> 00:04:17,540
+material، المواد المعلقة في سائل، are deposited in
+
+57
+00:04:17,540 --> 00:04:21,940
+the order of their weight، بتترسب بترتيب وزنها،
+
+58
+00:04:21,940 --> 00:04:27,440
+الأثقل على الإطلاق يكون في القاع، والأخف فيهم، أخف
+
+59
+00:04:27,440 --> 00:04:32,540
+شيء يكون موجود at the top، تمام؟ واحنا حكينا مش
+
+60
+00:04:32,540 --> 00:04:38,260
+why، إنه في حالة مثلاً عينة blood، الـ RBCs بتكون
+
+61
+00:04:38,260 --> 00:04:44,080
+موجودة at the bottom لأنها أثقل شيء، يليها أخف وزناً
+
+62
+00:04:44,080 --> 00:04:47,920
+منها طبقة الـ white BCs والـ platelets، ويليها الطبقة
+
+63
+00:04:47,920 --> 00:04:51,240
+الأخف وزناً على الإطلاق، التي هي البلازما في العينة
+
+64
+00:04:51,240 --> 00:04:55,580
+دي، فبالتالي بتكون هي موجودة على الـ top، تمام، أو على
+
+65
+00:04:55,580 --> 00:04:59,400
+الـ surface، على السطح، تمام. إذن the suspended
+
+66
+00:04:59,400 --> 00:05:03,680
+material، الأجزاء المعلقة أو الجزيئات المعلقة، بترتب
+
+67
+00:05:03,680 --> 00:05:08,060
+حسب وزنها، أثقل شيء في القاع، وأخف شيء at the top.
+
+68
+00:05:08,060 --> 00:05:12,760
+طبعاً there are many types of centrifuges، في عندي
+
+69
+00:05:12,760 --> 00:05:17,060
+أكثر من نوع من الـ centrifuges، ولكن كلها تقريباً
+
+70
+00:05:17,060 --> 00:05:22,160
+لها نفس مبدأ العمل طبعاً، وهي بتستخدم أو توظف الـ
+
+71
+00:05:22,160 --> 00:05:25,720
+centrifugal forces، التي هي قوة الطرد المركزي، في
+
+72
+00:05:25,720 --> 00:05:29,180
+فصل الجزيئات. طب خلونا نشوف مع بعض شو يعني
+
+73
+00:05:29,180 --> 00:05:34,610
+centrifugal forces، وكيف هي الأجهزة تطبق مبدأ الـ
+
+74
+00:05:34,610 --> 00:05:39,150
+centrifugal forces لحتى تفصل المواد. طيب، تمام. when
+
+75
+00:05:39,150 --> 00:05:43,570
+a body is rotated in a circular movement at speed,
+
+76
+00:05:43,570 --> 00:05:48,130
+centrifugal force is created that drives the body
+
+77
+00:05:48,130 --> 00:05:51,390
+away from the center of the circular movement.
+
+78
+00:05:51,390 --> 00:05:58,260
+تمام؟ هلأ لما بيكون في جسم body، تمام؟ بيتحرك حركة
+
+79
+00:05:58,260 --> 00:06:02,600
+دائرية circular movement at speed، بسرعة معينة،
+
+80
+00:06:02,600 --> 00:06:08,080
+حيكون بيؤثر على هذا الجسم قوة اسمها centrifugal
+
+81
+00:06:08,080 --> 00:06:12,620
+force، طبعاً؟ بت… هذا… بت… بت… بت… يعني
+
+82
+00:06:12,620 --> 00:06:17,580
+القوة هذه، لها نزعة بعيد عن المركز، يعني بت… بت…
+
+83
+00:06:17,580 --> 00:06:23,400
+بتحرك هذا الجسم أنه يروح بعيد عن المركز، طبعاً؟ إيش
+
+84
+00:06:23,400 --> 00:06:28,080
+يعني؟ هل في في الطبيعة أو في حولنا شيء يحاول
+
+85
+00:06:28,080 --> 00:06:32,580
+يوضح لنا معنى الكلام هذا؟ آه فيه، أكيد، كلنا روحنا
+
+86
+00:06:32,580 --> 00:06:37,320
+على مدينة الملاهي وكلنا لعبنا بلعبة السلاسل، طبعاً؟
+
+87
+00:06:37,320 --> 00:06:43,820
+لعبة السلاسل هي عبارة عن إيش؟ هي عبارة عن زي عمود
+
+88
+00:06:43,820 --> 00:06:51,570
+أو محور مركزي، تمام؟ وهذا المحور المركزي مرتبط
+
+89
+00:06:51,570 --> 00:06:56,550
+فيه عدة كراسي، هذه الكراسي هي الـ body، تمام؟ هي الـ
+
+90
+00:06:56,550 --> 00:07:01,990
+body، تمام؟ لما بت… بنشغل لعبة السلاسل، والـ
+
+91
+00:07:01,990 --> 00:07:06,670
+body هذا بيبدأ يتحرك بـ circular movement، بيبدأ
+
+92
+00:07:06,670 --> 00:07:10,690
+يصير في حركة دائرية وسرعة لهذه الكراسي، ساعتها إيش
+
+93
+00:07:10,690 --> 00:07:14,290
+بيصير؟ بنلاحظ أن هذه الكراسي بعدت عن المركز،
+
+94
+00:07:14,290 --> 00:07:17,550
+صح؟ بتصير بعيدة، بتصير طايرة في الهواء، تمام؟
+
+95
+00:07:17,550 --> 00:07:22,420
+نتيجة بعدها عن المركز، ما الذي أبعدها عن المركز؟ هي
+
+96
+00:07:22,420 --> 00:07:26,700
+الـ centrifugal forces، القوة التي طردتها من المركز،
+
+97
+00:07:26,700 --> 00:07:34,240
+قوة الطرد المركزي، تمام؟ نفس الشيء يحدث في الجهاز
+
+98
+00:07:34,240 --> 00:07:38,360
+الـ centrifuge، أنا بحط الـ body التي هي الأنابيب،
+
+99
+00:07:38,360 --> 00:07:43,880
+أنابيب العينات، وبتحرك بحركة دائرية بسرعة
+
+100
+00:07:43,880 --> 00:07:47,620
+فبالتالي هذه الأنابيب إيش مالها؟ بتبعد، بتصير
+
+101
+00:07:47,620 --> 00:07:51,960
+بعيدة، بتصير بعيدة عن المركز، منتشرة، تمام؟
+
+102
+00:07:51,960 --> 00:07:59,370
+فبالتالي بيفصل محتوياتها بحيث إنه إيش؟ الأثقل شيء
+
+103
+00:07:59,370 --> 00:08:03,310
+بيكون… بيروح… بيروح أبعد ما يمكن عن المركز،
+
+104
+00:08:03,310 --> 00:08:07,630
+والأخف شيء بيكون أقرب ما يمكن من المركز. إيش معنى
+
+105
+00:08:07,630 --> 00:08:13,510
+هذا الكلام؟ يعني أبعد شيء عن المركز هو أثقل جزء في
+
+106
+00:08:13,510 --> 00:08:17,070
+العينة، يعني بيكون at the bottom of the tube، في
+
+107
+00:08:17,070 --> 00:08:23,970
+قاع الـ tube، طبعاً. ولكن الجزء الذي يكون خفيف، light،
+
+108
+00:08:23,970 --> 00:08:29,750
+وزنه خفيف، بيكون قريب من المركز، يعني بيكون at the
+
+109
+00:08:29,750 --> 00:08:33,890
+top of the tube، على سطح الـ tube أو في السطح العلوي
+
+110
+00:08:33,890 --> 00:08:38,960
+من الـ tube. طبعاً هذه فكرة عمل الـ centrifuge، وهي
+
+111
+00:08:38,960 --> 00:08:43,460
+معنى كلمة centrifugal forces، قوة تطرد الـ objects،
+
+112
+00:08:43,460 --> 00:08:48,920
+تطرد الـ body بعيداً عن المركز أثناء دورانه بحركة
+
+113
+00:08:48,920 --> 00:08:53,930
+دائرية بسرعة معينة، طبعاً؟ the greater the
+
+114
+00:08:53,930 --> 00:08:57,890
+outward pull due to rotation that is centrifugal
+
+115
+00:08:57,890 --> 00:09:01,730
+force, the more rapid and effective is the
+
+116
+00:09:01,730 --> 00:09:06,090
+sedimentation. طبعاً كل ما زاد السحب أو البعد أو الطرد
+
+117
+00:09:06,090 --> 00:09:10,830
+بعيداً عن المركز، طبعاً؟ معناه بيكون في عندي أكثر
+
+118
+00:09:10,830 --> 00:09:16,210
+سرعة وأكثر فعالية للفصل، للـ sedimentation، لترسيب
+
+119
+00:09:16,210 --> 00:09:21,930
+الأجزاء الثقيلة، طبعاً؟ طيب، as a result، فبالتالي
+
+120
+00:09:21,930 --> 00:09:27,370
+ينتج عن ذلك heavier elements are thrown to the
+
+121
+00:09:27,370 --> 00:09:32,470
+bottom of the tube، الأجزاء الثقيلة بتكون أبعد ما
+
+122
+00:09:32,470 --> 00:09:36,930
+يمكن عن المركز، يعني بتكون في the bottom of the tube،
+
+123
+00:09:36,930 --> 00:09:40,290
+في قاع الـ tube، لأن قاع الـ tube هو بيكون أبعد جزء
+
+124
+00:09:40,290 --> 00:09:44,830
+عن المركز، تمام؟ followed by lighter particles، وبعدها
+
+125
+00:09:44,830 --> 00:09:49,530
+يليها الأجزاء الخفيفة التي تكون at the top
+
+126
+00:09:49,530 --> 00:09:52,670
+of the tube، يعني بتكون قريبة نوعاً ما من المركز.
+
+127
+00:09:52,670 --> 00:09:57,750
+لحتى نفهم الكلام الذي حكيناه، خلونا نشوف صورة الـ
+
+128
+00:09:57,750 --> 00:10:01,830
+centrifuge، ونعرف كيف بيشتغل. هذه الـ centrifuge، و
+
+129
+00:10:01,830 --> 00:10:05,370
+موجود فيه، فرضاً، موجود حطينا فيه أنابيب. هذا الـ
+
+130
+00:10:05,370 --> 00:10:10,630
+centrifuge وشغلناه، صار يتحرك حركة دائرية بسرعة
+
+131
+00:10:10,630 --> 00:10:14,260
+معينة، راح يؤثر عليه قوة طرد مركزي، إيش يعني؟ يعني
+
+132
+00:10:14,260 --> 00:10:17,920
+الأنابيب هذه إيش مالها؟ راح تبعد عن المركز، إيش
+
+133
+00:10:17,920 --> 00:10:20,620
+يعني راح تبعد عن المركز؟ يعني قاعها راح يبعد
+
+134
+00:10:20,620 --> 00:10:25,800
+عن المركز، راح تتحرك بعيداً عن المركز، وهذه الأنابيب
+
+135
+00:10:25,800 --> 00:10:29,940
+إذا قاعها بيكون بعيداً عن المركز، طبعاً؟ طيب،
+
+136
+00:10:30,900 --> 00:10:34,240
+تبعاً لقوة الطرد المركزي، centrifugal force، as a result
+
+137
+00:11:00,990 --> 00:11:06,270
+تمام؟ هي معناه أنني أفصل عينة باستخدام الـ
+
+138
+00:11:06,270 --> 00:11:09,550
+centrifuge، وتطبيق الـ centrifugal forces، التي هي
+
+139
+00:11:09,550 --> 00:11:15,710
+قوة الطرد المركزي، تمام؟ طيب، centrifugal forces
+
+140
+00:11:15,710 --> 00:11:19,870
+increase with the speed of rotation, that is
+
+141
+00:11:19,870 --> 00:11:23,290
+revolutions of the rotor per minute and the radius
+
+142
+00:11:23,290 --> 00:11:28,560
+of rotation. هلأ قوة الطرد المركزي بت… تزيد كل ما
+
+143
+00:11:28,560 --> 00:11:32,820
+زادت السرعة، والسرعة نعبر عنها revolutions per
+
+144
+00:11:32,820 --> 00:11:36,240
+minute أو rounds per minute، كم دقيقة… كم لفة
+
+145
+00:11:36,240 --> 00:11:41,660
+يلف الجهاز بالدقيقة الواحدة، هي سرعته، تمام؟ و
+
+146
+00:11:41,660 --> 00:11:46,060
+أيضاً قطر الجهاز، لأنه إحنا كل ما كان قطره كبير، يعني
+
+147
+00:11:46,060 --> 00:11:51,340
+فيه، حيكون إيش؟ حيكون… حيصير طرد بعيداً عن المركز،
+
+148
+00:11:51,340 --> 00:11:55,920
+تمام؟ وحيكون… ليه قوة الطرد المركزي أكثر، لذا
+
+149
+00:11:55,920 --> 00:12:04,840
+قوة الطرد المركزي بتتأثر يا بنات بال… بال… السرعة
+
+150
+00:12:04,840 --> 00:12:11,360
+… سرعة الجهاز، وأيضاً بالقطر، قطر الجهاز، تمام؟
+
+151
+00:12:11,360 --> 00:12:17,640
+هذا يعني بتتأثر بسرعة الجهاز وقطر الجهاز، تمام؟
+
+152
+00:12:17,640 --> 00:12:24,660
+إذن هذا بالنسبة لمن؟ للـ centrifugal forces.
+
+153
+00:12:24,660 --> 00:12:30,500
+the actual sedimentation achieved at a given speed
+
+154
+00:12:30,500 --> 00:12:34,120
+depends therefore on the radius of centrifugation.
+
+155
+00:12:34,120 --> 00:12:40,360
+هلأ لو أنا بدي أثبت السرعة، a given speed، يعني سرعة
+
+156
+00:12:40,360 --> 00:12:44,560
+معينة، معناها الـ sedimentation ح تعتمد على مين؟
+
+157
+00:12:44,560 --> 00:12:47,840
+ح تعتمد على الـ radius، على القطر. يعني الـ centrifugal
+
+158
+00:12:47,840 --> 00:12:51,180
+forces، طالما هي بتعتمد على السرعة والـ radius، إذن
+
+159
+00:12:51,180 --> 00:12:55,160
+السرعة ثابتة، معناها بتعتمد على الـ radius، طبعاً؟ طيب،
+
+160
+00:12:55,160 --> 00:13:00,020
+إذن يعني شيء واضح ومنطقي. most techniques
+
+161
+00:13:00,020 --> 00:13:03,460
+requiring centrifugation will usually specify that
+
+162
+00:13:03,460 --> 00:13:07,080
+require a relative centrifugal force expressed in
+
+163
+00:13:07,080 --> 00:13:12,810
+g’s (gravity). هلأ معظم التقنيات والتجارب والفحوصات التي
+
+164
+00:13:12,810 --> 00:13:17,370
+إحنا بنستخدمها، لابد أن نمر بمرحلة فصل للعينة لحتى
+
+165
+00:13:17,370 --> 00:13:21,370
+نحصل على مثلاً plasma، نحصل على serum إذا أنا
+
+166
+00:13:21,370 --> 00:13:25,550
+بستخدم blood، أو نحصل على sediment إذا أنا بستخدم
+
+167
+00:13:25,550 --> 00:13:30,490
+عينة urine أو stool أو whatever، تمام؟ لازم أنا قبل
+
+168
+00:13:30,490 --> 00:13:34,550
+ما أجري الفحص أستخدم إيش؟ أستخدم الـ…
+
+169
+00:13:37,540 --> 00:13:41,440
+يعني قبل ما أجري الفحص، أستخدم الـ centrifuge حتى أفصل
+
+170
+00:13:41,440 --> 00:13:48,140
+العينة تبعي، طبعاً. طيب، التجربة تبعي بيكون محدد لي
+
+171
+00:13:48,140 --> 00:13:52,640
+أو محدد لي أن أفصل على سرعة كذا أو باستخدام
+
+172
+00:13:52,640 --> 00:13:57,660
+relative centrifugal forces قيمتها كذا، طبعاً. و دائماً
+
+173
+00:13:57,660 --> 00:14:00,660
+بيعبّر عن الـ relative centrifugal forces على إنها
+
+174
+00:14:00,660 --> 00:14:05,560
+هي بدلالة الـ g’s،  احنا حكينا هي أضعاف قوة الجاذبية،
+
+175
+00:14:05,5
+
+223
+00:17:46,050 --> 00:17:50,030
+القنابيب إذا حُطّت وموجودة على الـ head خلاص طالما
+
+224
+00:17:50,030 --> 00:17:53,630
+أنّه لفّ الـ .. الـ .. الـ central shaft إذا صار يدور
+
+225
+00:17:53,630 --> 00:17:57,650
+معناها القنابيب صارت تدور معه، تمام؟ طيب الجزء
+
+226
+00:17:57,650 --> 00:18:00,470
+الثالث اللي هي الـ buckets أو الـ tubes حكينا إنها
+
+227
+00:18:00,470 --> 00:18:06,050
+هذه قنابيب مصممة أو buckets مصممة لتستقبل قنابيب
+
+228
+00:18:06,050 --> 00:18:10,770
+العينة إذا إن هي عبارة عن a portion جزء، تمام؟ that
+
+229
+00:18:10,770 --> 00:18:15,010
+hold test tubes بتثبت أنا بِها بالفحص أو أنا بِها
+
+230
+00:18:15,010 --> 00:18:18,030
+باللي أنا بشتغل فيها اللي فيها عينتي to best band
+
+231
+00:18:18,030 --> 00:18:22,130
+طبعاً الجهاز الـ centrifuge يا بنات بيكون طبعاً هذا
+
+232
+00:18:22,130 --> 00:18:26,650
+الجزء أنا ما بشوفه ده، يعني ما بيكون مرئي هنا يعني
+
+233
+00:18:26,650 --> 00:18:32,190
+زي يعملي زي كأنه نازع الجزء الأمامي من الجهاز عشان
+
+234
+00:18:32,190 --> 00:18:37,290
+يشوفها يعني هذه رسمة توضيحية لمحتويات الجهاز، ولكن
+
+235
+00:18:37,290 --> 00:18:40,370
+أنا لما بشوف .. بس بشوف الـ .. الـ .. يعني أنا لما
+
+236
+00:18:40,370 --> 00:18:43,490
+بدي أُلقي نظرة بأُلقي نظرة علوية على الجهاز من هنا
+
+237
+00:18:43,490 --> 00:18:48,970
+تمام، من الفتحة تبعته بشوف بس الـ head و الـ .. الـ
+
+238
+00:18:48,970 --> 00:18:52,150
+buckets تمام؟ ما بشوفش الـ shaft الـ central shaft
+
+239
+00:18:52,150 --> 00:18:59,950
+ما بشوفه تمام؟ إذا هذه هي مكونات الجهاز، طيب نيجي
+
+240
+00:18:59,950 --> 00:19:03,650
+لأنواع الـ .. الـ centrifuge classification of
+
+241
+00:19:03,650 --> 00:19:07,730
+centrifuge تصنيف الـ centrifuge أو أنواعه، طبعاً حسب
+
+242
+00:19:07,730 --> 00:19:12,810
+على .. على ماذا يُدار هذا الجهاز أو كيف يتم إدارة
+
+243
+00:19:12,810 --> 00:19:18,190
+أو تشغيل هذا الجهاز، يُصنّف الـ centrifuges إلى hand
+
+244
+00:19:18,190 --> 00:19:22,980
+centrifuges و electrical centrifuges، من اسمها hand
+
+245
+00:19:22,980 --> 00:19:27,720
+centrifuges إذا .. إذا تُدار باستخدام .. إيش؟
+
+246
+00:19:27,720 --> 00:19:31,780
+باستخدام اليد، أما الـ .. الـ electrical centrifuges
+
+247
+00:19:31,780 --> 00:19:35,980
+تُدار باستخدام الكهرباء طبعاً؟ طيب خلينا نشوف إيش
+
+248
+00:19:35,980 --> 00:19:40,080
+يعني hand centrifuges زي ما أنتو شايفين هي عبارة
+
+249
+00:19:40,080 --> 00:19:44,300
+عن old fashion of centrifuge يعني الـ centrifuge
+
+250
+00:19:44,300 --> 00:19:51,060
+هيك موديل قديم، دقة قديمة، تمام؟ ويعني الـ .. الـ ..
+
+251
+00:19:51,060 --> 00:19:54,780
+الـ .. موديل القديم من الـ centrifuge بـ .. بـ .. يُتمّ
+
+252
+00:19:54,780 --> 00:19:57,980
+تشغيله باستخدام الـ hand يعني باليدوي، تمام؟ أنا
+
+253
+00:19:57,980 --> 00:20:03,290
+بحرك الـ .. اليد هادي وبيصير يدور تمام؟ هي زي ما
+
+254
+00:20:03,290 --> 00:20:06,370
+أنتم شايفين الـ central shaft و head هي الدائرة
+
+255
+00:20:06,370 --> 00:20:09,970
+اللي عاملة زي الـ cross و الـ buckets هي هي طبعاً
+
+256
+00:20:09,970 --> 00:20:14,150
+هادِ أنا بحط فيها القنابيب تبعتي، طيب إذا يُتمّ
+
+257
+00:20:14,150 --> 00:20:17,490
+تشغيله إما بالـ hand أو بالـ water pressure يعني
+
+258
+00:20:17,490 --> 00:20:20,950
+ممكن ضغط ماء يأثر على اليد هادي و يصير يحركها
+
+259
+00:20:20,950 --> 00:20:25,570
+فاتلف ويلف معها الجهاز ويتم الفصل، طبعاً بما أن
+
+260
+00:20:25,570 --> 00:20:28,610
+old fashion معناها بيستخدم في الـ small
+
+261
+00:20:28,610 --> 00:20:34,170
+laboratories كان يُستخدم قديماً، بيستخدم ممكن في الـ
+
+262
+00:20:34,170 --> 00:20:37,870
+routine purposes، إيش يعني routine purposes؟ لما
+
+263
+00:20:37,870 --> 00:20:44,510
+أحكي routine test أو routine lab work يعني urine
+
+264
+00:20:44,510 --> 00:20:50,250
+and stool analysis طبعاً؟ إذا يُستخدم هذا النوع من
+
+265
+00:20:51,010 --> 00:20:55,410
+الـ .. الـ .. الـ centrifuges في الـ routine work يعني
+
+266
+00:20:55,410 --> 00:20:58,810
+في الـ urinary sediment عشان أُحضّر الـ urinary
+
+267
+00:20:58,810 --> 00:21:02,210
+sediment أُفصل عينة الـ urine عشان أعمل
+
+268
+00:21:02,210 --> 00:21:05,110
+concentration للـ parasites اللي موجود في الـ stool
+
+269
+00:21:05,110 --> 00:21:08,730
+تمام؟ إذا عينات الـ urine هو الـ stool اللي بيستخدم
+
+270
+00:21:08,730 --> 00:21:15,030
+it is not advisable، not advisable مش يعني غير مُوصى
+
+271
+00:21:15,030 --> 00:21:19,340
+أو غير .. لا يُنصح بأن هذا الجهاز يُستخدم عشان أُفصل
+
+272
+00:21:19,340 --> 00:21:25,260
+عينة دم فيه، ليش؟ لإنه أنا بأستخدم يدوي وحركة يدي
+
+273
+00:21:25,260 --> 00:21:28,820
+مش منتظمة، إذا الـ relative centrifugal forces
+
+274
+00:21:28,820 --> 00:21:33,220
+هتكون irregular، طالما إنه كانت القوة irregular
+
+275
+00:21:33,220 --> 00:21:37,660
+معناها عملية الفصل غير منتظمة، يعني ممكن الـ RBCs و
+
+276
+00:21:37,660 --> 00:21:41,860
+هي عمّ تنفصل وهي عمّ بتتجه باتجاه الـ sediment تتكسر
+
+277
+00:21:42,510 --> 00:21:45,690
+بالتالي الـ serum أو الـ plasma اللي أنا بدي أخدها
+
+278
+00:21:45,690 --> 00:21:50,810
+بدل ما يكون لونها لون أصفر و قوي strong color و
+
+279
+00:21:50,810 --> 00:21:56,570
+تكون clear ممكن تتحول لونها للون الأحمر نتيجة تكسر
+
+280
+00:21:56,570 --> 00:22:02,350
+الـ RBCs وأيضاً خروج محتويات الـ RBCs على الـ serum
+
+281
+00:22:02,350 --> 00:22:06,710
+أو على الـ plasma ممكن يؤثر على عدة فحوصات وتصبح
+
+282
+00:22:06,710 --> 00:22:11,870
+العينة غير صالحة لإجراء الفحوصات تمام؟ فبالتالي إذا
+
+283
+00:22:11,870 --> 00:22:16,110
+لا يُنصح به لفصل عينة دم، خلينا نشوف إيش يعني
+
+284
+00:22:16,110 --> 00:22:20,450
+electrical centrifuges، electrical centrifuges هي
+
+285
+00:22:20,450 --> 00:22:23,610
+عبارة عن الـ centrifuges that are operated by
+
+286
+00:22:23,610 --> 00:22:27,890
+electrical power تمام؟ يُتمّ إدارتها باستخدام
+
+287
+00:22:27,890 --> 00:22:33,070
+الكهرباء and produce high centrifugal force بـ ..
+
+288
+00:22:33,070 --> 00:22:41,000
+يعني .. بتنتج يعني قوة طاردة مركزية عالية جداً و
+
+289
+00:22:41,000 --> 00:22:44,860
+بتستخدم في الـ most medical laboratories و بتستخدم
+
+290
+00:22:44,860 --> 00:22:49,200
+أيضاً لفصل عينات الدم، يعني بتعطيني إنّه يعني فصل
+
+291
+00:22:49,200 --> 00:22:55,570
+دقيق و فصل يعني جيد تمام؟ هذا بالنسبة لـ .. how it
+
+292
+00:22:55,570 --> 00:22:59,870
+operated كيف أنا ممكن أشغّل الجهاز by hand or by
+
+293
+00:22:59,870 --> 00:23:04,590
+electric .. electric أو electricity تمام؟ طيب، هلّأ
+
+294
+00:23:04,590 --> 00:23:10,470
+بالنسبة لحجم الـ centrifuge، في عندي أنواع منه، في
+
+295
+00:23:10,470 --> 00:23:14,310
+micro centrifuge يعني بيستخدم أنابيب صغيرة أو
+
+296
+00:23:14,310 --> 00:23:18,490
+بسمّيه zero centrifuge هذا يا بنات بيفصل، بينستخدمه
+
+297
+00:23:18,490 --> 00:23:22,750
+for spinning لفصل small tubes يعني أنا العينات
+
+298
+00:23:22,750 --> 00:23:25,410
+تبعتي بتكون موجودة في الـ small tubes زي الـ
+
+299
+00:23:25,410 --> 00:23:28,570
+centrifuges اللي بتكون موجودة في الـ blood bank
+
+300
+00:23:28,570 --> 00:23:32,990
+laboratories في مختبرات اللي هو بنوك الدم طبعاً
+
+301
+00:23:32,990 --> 00:23:39,100
+خلينا نشوف إيش يعني الـ .. الـ .. الـ small tubes، هلّأ
+
+302
+00:23:39,100 --> 00:23:42,520
+في عندي tube بسمّيها eppendorf tube هي صغيرة كتير
+
+303
+00:23:42,520 --> 00:23:45,480
+يعني إنها بتاخد بس واحد ملّي، واحد ملّي أو واحد و
+
+304
+00:23:45,480 --> 00:23:49,940
+نصف ملّي تمام هي زي ما أنتم شايفين هنا هي مدرّجة و
+
+305
+00:23:49,940 --> 00:23:55,040
+بتاخد يعني واحد ملّي أو واحد ونصف ملّي، فصغيرة
+
+306
+00:23:55,040 --> 00:23:59,220
+كتير، محتواها صغير، العينة تبعتي اللي هتكون فيها small
+
+307
+00:23:59,220 --> 00:24:04,250
+amount، فبالتالي هي small tubes، فالجهاز تبعي الـ
+
+308
+00:24:04,250 --> 00:24:08,530
+centrifuge بسمّي اللي بيفصل أو بيستقبل هاي القنابيب
+
+309
+00:24:08,530 --> 00:24:12,110
+وبفصلها بسمّيه micro centrifuge أو sericentrifuge
+
+310
+00:24:12,110 --> 00:24:14,310
+معناها هذا الـ micro centrifuge أو الـ
+
+311
+00:24:14,310 --> 00:24:17,190
+sericentrifuge الـ bucket تبعته هتكون صغيرة مصمّمة
+
+312
+00:24:17,190 --> 00:24:23,040
+على جِد عشان الـ .. الـ .. الـ eppendorf tube تمام؟ طيب
+
+313
+00:24:23,040 --> 00:24:27,660
+الـ .. بالنسبة لـ الـ medium-sized centrifuge حجم
+
+314
+00:24:27,660 --> 00:24:31,000
+متوسط يعني بنستخدمه لفصل عينات الـ urine، ممكن
+
+315
+00:24:31,000 --> 00:24:35,420
+لعينات الـ blood، بيستخدم medium-sized tubes، بيفصل
+
+316
+00:24:35,420 --> 00:24:38,080
+medium-sized tubes اللي هي بنسمّيها centrifuge
+
+317
+00:24:38,080 --> 00:24:43,120
+tubes اللي أنا بتبعته بسمّيها centrifuge tubes زي
+
+318
+00:24:43,120 --> 00:24:47,900
+كيف شكلها، ممكن يكون هيك شكلها مثلاً هذه ممكن أسمّيها
+
+319
+00:24:47,900 --> 00:24:52,200
+هي falcon tube و إلها غطاية، هي اسمها falcon tube
+
+320
+00:24:52,200 --> 00:24:56,740
+تمام، و إلها غطاية، ولكن هي centrifuge tube، نحطها في
+
+321
+00:24:56,740 --> 00:25:00,240
+الـ centrifuge و بتفصل تمام، ولكن مش هي الأكثر
+
+322
+00:25:00,240 --> 00:25:03,720
+شيوعاً، في عندي نوع من القنابيب أكثر شيوعاً زيها بس
+
+323
+00:25:03,720 --> 00:25:08,020
+ما إلها غطاية، هلّأ بورجيكم إياها تمام
+
+324
+00:25:12,270 --> 00:25:18,370
+وينو كان موجود من شوي هنا، هاي .. هذا بسمّيه
+
+325
+00:25:18,370 --> 00:25:22,470
+centrifuge tube تمام؟ و بنستخدم .. و هنستخدمه كتير
+
+326
+00:25:22,470 --> 00:25:28,850
+في المختبرات، طبعاً medium size تمام؟ طيب، لكن الـ
+
+327
+00:25:28,850 --> 00:25:33,110
+.. الـ .. الـ large centrifuges الـ .. الـ centrifuges
+
+328
+00:25:33,110 --> 00:25:36,290
+الكبيرة بتاخد أنابيب كتير كبيرة يا بنات، يعني هاي
+
+329
+00:25:36,290 --> 00:25:42,940
+مثلاً ممكن زي هاي الأنابيب، شايفين هنا الحجم تبعها
+
+330
+00:25:42,940 --> 00:25:50,280
+بيصل لخمسين ملّي يعني، تمام؟ هذا هو الحجم اللي
+
+331
+00:25:50,280 --> 00:25:54,640
+أنا بِحط فيه أنابيب كبيرة، ممكن يعني بياخد لخمسين ملّي،
+
+332
+00:25:54,640 --> 00:25:59,500
+ممكن فلاسكات، تيوبات حجمها كبير، فبالتالي هذه
+
+333
+00:25:59,500 --> 00:26:03,080
+بسمّيها إيش؟ Large Centrifuges، نستخدمها في الـ
+
+334
+00:26:03,080 --> 00:26:07,640
+bacteriology أو أحياناً كمان في الـ cytology في الـ
+
+335
+00:26:07,640 --> 00:26:15,420
+.. الـ ..  أبحاث الخلايا و الـ .. يعني علم الخلايا و
+
+336
+00:26:15,420 --> 00:26:18,900
+العلم الـ micro part لو أنا مثلاً عاملة enrichment
+
+337
+00:26:18,900 --> 00:26:22,800
+عاملة cultivation .. culture لبكتيريا، زراعة
+
+338
+00:26:22,800 --> 00:26:27,340
+بكتيريا بكمية، بكميات يعني في .. في أنابيب كبيرة و
+
+339
+00:26:27,340 --> 00:26:30,160
+هلّأ بدي أُفصل الـ .. أنا الـ bacteria بحيث أني
+
+340
+00:26:30,160 --> 00:26:33,320
+أُرسبها وأستخدمها، أعمل عليها تطبيقات، بأستخدم الـ
+
+341
+00:26:33,320 --> 00:26:38,000
+large centrifuge، تمام؟ طيب، هذا بالنسبة لمين؟ لـ
+
+342
+00:26:38,000 --> 00:26:43,160
+الـ .. الـ أنواع الـ centrifuges حسب حجمها، تمام؟ أي
+
+343
+00:26:43,160 --> 00:26:48,160
+جهاز أنا بدي أستخدمه طبعاً لازم في عندي تعليمات كيف
+
+344
+00:26:48,160 --> 00:26:51,960
+أنا أستخدمه وكيف أنا أعتني بهذا الجهاز وأحافظ
+
+345
+00:26:51,960 --> 00:26:55,760
+عليه، أول إشي reading of the manufacturer
+
+346
+00:26:55,760 --> 00:27:00,520
+instruction لما أنا أقرأ تعليمات الجهة المصنّعة لهذا
+
+347
+00:27:00,520 --> 00:27:05,280
+الجهاز، الكتيب اللي بيجي مع الجهاز، ساعتها أنا عرفت
+
+348
+00:27:05,280 --> 00:27:09,500
+كيف أستخدمه، كيف أحافظ عليه، كيف أنا يعني المحاذير
+
+349
+00:27:09,500 --> 00:27:14,640
+اللي بتكون يعني مرفقة مع هذا الجهاز، طبعاً؟ طيب، و
+
+350
+00:27:14,640 --> 00:27:21,560
+أيضاً لازم كجهاز centrifuge أنا أعمله placing على
+
+351
+00:27:21,560 --> 00:27:25,140
+firm level bench، إيش يعني؟ يعني أحطه على bench
+
+352
+00:27:25,140 --> 00:27:29,320
+ثابت، غير .. يعني مش .. غير قابل للاهتزاز أو الحركة
+
+353
+00:27:29,320 --> 00:27:35,760
+أو إنّه ممكن الجهاز يقع منه، طبعاً؟ وأيضاً لإنه يا
+
+354
+00:27:35,760 --> 00:27:41,300
+بنات لما إحنا بنشغل هذا الجهاز بيعطيني ذبذبات أو
+
+355
+00:27:41,300 --> 00:27:46,320
+vibrations، اهتزازات لو ما كانش على بنش ثابت و
+
+356
+00:27:46,320 --> 00:27:51,420
+الجهاز موجود يعني ثابت على الـ .. الـ bench، فممكن
+
+357
+00:27:51,420 --> 00:27:56,160
+إنّه يتحرك ويقع تمام؟ وأيضاً لازم أنا أُبعده عن الـ
+
+358
+00:27:56,160 --> 00:28:01,890
+sunlight، عن ضوء الشمس، يخرب، وبحطه داخل الـ bench
+
+359
+00:28:01,890 --> 00:28:05,630
+يعني مش على طرف الـ bench لإنه مع الاهتزازات ممكن
+
+360
+00:28:05,630 --> 00:28:09,930
+إنّه يقع، الجهاز always closing the centrifuge
+
+361
+00:28:09,930 --> 00:28:15,010
+before turning it on، ده طبعاً لازم أن أنا أسكر
+
+362
+00:28:15,010 --> 00:28:18,990
+غطاية الـ centrifuge لما بدي أشغّل الـ centrifuge
+
+363
+00:28:18,990 --> 00:28:24,220
+لازم أنا أسكر الـ cover تبعه، بعدين أشغّله، ليش؟ لإنه
+
+364
+00:28:24,220 --> 00:28:27,880
+لو أنا شغّلته وهو فاتح ممكن تطاير الأنابيب أو يطاير
+
+365
+00:28:27,880 --> 00:28:32,820
+محتوياتها أو ينكب نتيجة فعل الـ .. يعني الـ rotation،
+
+366
+00:28:32,820 --> 00:28:37,900
+تمام؟ فبالتالي خطأ إن أنا أشغّله وهو فاتح، لازم أسكر
+
+367
+00:28:37,900 --> 00:28:43,480
+الجهاز قبل ما أشغّله، Always balancing the tubes that
+
+368
+00:28:43,480 --> 00:28:47,890
+are being centrifuged، هلّأ طبعاً لازم أنا أعمل
+
+369
+00:28:47,890 --> 00:28:52,270
+balance للأنابيب على مرجوحة الـ .. الـ .. السلاسل
+
+370
+00:28:52,270 --> 00:28:55,590
+لما بيجيبوا بيـ .. بيجلسوا الناس اللي جاية تلعب
+
+371
+00:28:55,590 --> 00:29:00,850
+بيجلسوهم كل اثنين مقابل بعضهم، وبيكونوا طبعاً نفس
+
+372
+00:29:00,850 --> 00:29:04,610
+الحجم أو نفس الوزن، يعني نفس الكتلة، تمام؟ يُفضّل،
+
+373
+00:29:04,610 --> 00:29:09,000
+يُراعَى، هيك طبعاً، فنفس الفكرة في جهاز الـ centrifuge
+
+374
+00:29:09,000 --> 00:29:13,640
+لما أنا بدي أحط القنابيب داخل الـ centrifuge لازم
+
+375
+00:29:13,640 --> 00:29:17,440
+أحطها balance، إيش يعني؟ كل tube مقابلها tube نفس
+
+376
+00:29:17,440 --> 00:29:21,800
+الشكل ونفس الحجم ونفس المحتوى، طبعاً بحطهم مقابل
+
+377
+00:29:21,800 --> 00:29:25,770
+بعضهم البعض، طب إذا أنا ما عملت balancing راح يصير
+
+378
+00:29:25,770 --> 00:29:29,950
+فيه اهتزازات للجهاز، ممكن يقع، ممكن تتكسر الأنابيب
+
+379
+00:29:29,950 --> 00:29:35,110
+في الداخل أو ينكب محتواها، تمام؟ وبيعطي صوت يعني
+
+380
+00:29:35,110 --> 00:29:38,830
+إزعاج، بيبطل يستوعب الجهاز، نتيجة أنّه ما فيش
+
+381
+00:29:38,830 --> 00:29:44,890
+balance، تمام؟ فإذا جزئية الـ balance أو نقطة الـ
+
+382
+00:29:44,890 --> 00:29:48,630
+balance مهمّة جداً يا بنات، الـ tubes should also be
+
+383
+00:29:48,630 --> 00:29:51,230
+the same size and should contain the same amount
+
+384
+00:29:51,230 --> 00:29:53,610
+الأنابيب اللي أنا بدي أحطها بالمقابل بعضها البعض
+
+385
+00:29:53,610 --> 00:29:58,770
+لازم تكون نفس الحجم ومحتواها أيضاً نفس الكمية، طب أنا
+
+386
+00:29:58,770 --> 00:30:05,030
+عندي أنبوب واحدة فيها 5 cm أو 5 ملّي من الـ blood
+
+387
+00:30:05,030 --> 00:30:08,790
+طب أنا كيف بدي أُفصلها؟ كيف بدي أعمل لها balance؟
+
+388
+00:30:08,790 --> 00:30:12,590
+مالهاش balance أحطها لحالها؟ لأ، يستحسن أحطها لحالها
+
+389
+00:30:12,950 --> 00:30:17,830
+لازم أحط tube .. أجيب tube نفس الشكل ونفس الحجم و
+
+390
+00:30:17,830 --> 00:30:23,150
+أحط فيها خمسة ملّي water، تمام؟ distilled water أو
+
+391
+00:30:23,150 --> 00:30:27,290
+water .. tap water تمام؟ وأحطهم مقابل بعضهم البعض
+
+392
+00:30:27,290 --> 00:30:33,150
+في الجهاز، نهائياً الجهاز ما بيستوعب ولا بيشتغل إذا
+
+393
+00:30:33,15
+
+445
+00:34:25,080 --> 00:34:30,440
+ويقف لحاله طبعا في timer في الجهاز أنا بحدد السرعة
+
+446
+00:34:30,440 --> 00:34:35,040
+اللي بدي إياها هنا، وبحدد ال timer يعني كام وقت
+
+447
+00:34:35,040 --> 00:34:38,200
+الفصل five minutes، بعد الجهاز عن five minutes بس
+
+448
+00:34:38,200 --> 00:34:44,460
+يخلصه، بيبدأ...بي... بيعطيني run هذا الجرس تبعه
+
+449
+00:34:44,460 --> 00:34:47,760
+بيعطيني run أنه خلاص توقف الفصل وتوقف الجهاز و
+
+450
+00:34:47,760 --> 00:34:53,580
+خلصت المدة، فبتبدأ تتباطأ حركته إلى أن يقف لحاله
+
+451
+00:34:53,580 --> 00:34:58,850
+طبعا؟ هذا بالنسبة لجهاز ال centrifuge إن شاء الله
+
+452
+00:34:58,850 --> 00:35:03,290
+كتطبيق على جهاز ال centrifuge راح نأخذ فحص اسمه
+
+453
+00:35:03,290 --> 00:35:07,830
+hematocrit تمام؟ أو نسبة ال RBC's، نسبة...نسبة
+
+454
+00:35:07,830 --> 00:35:11,270
+المئوية للـ RBC's من إيش؟ من ال whole blood
+
+455
+00:35:11,270 --> 00:35:15,750
+volume يعني قد إيش بتحتل ال RBC's من ال whole volume
+
+456
+00:35:15,750 --> 00:35:19,730
+ده أعتبر أن العينة ال blood كلها 100%، وأشوف ال
+
+457
+00:35:19,730 --> 00:35:24,170
+RBC's كام في المية من هذه العينة تمام؟ إحنا أخذنا
+
+458
+00:35:24,170 --> 00:35:28,150
+أنه هي theoretical 45%، بنشوف عند...في ال...في
+
+459
+00:35:28,150 --> 00:35:31,890
+البناء آدمي كام بتكون نسبتها أو يعني كام ال range
+
+460
+00:35:31,890 --> 00:35:38,060
+طبعا تمام هذا ال...التطبيق العملي رح يكون يعني
+
+461
+00:35:38,060 --> 00:35:42,100
+أنا راح أسحب عينة الدم، راح أحطها في tube طبعا ال...
+
+462
+00:35:42,100 --> 00:35:46,060
+ال...ال...هذا شرح عشان ي...يقرب لي الفكرة ولكن
+
+463
+00:35:46,060 --> 00:35:49,820
+أنه أنا بدي أحطها في tube خاصة في الفحص ال
+
+464
+00:35:49,820 --> 00:35:53,500
+hematocrite وبعدين بحطها في جهاز centrifuge أصلا
+
+465
+00:35:53,500 --> 00:35:57,870
+أيضا خاص في...فحص ال hematocrit بسميه hematocrit
+
+466
+00:35:57,870 --> 00:36:02,350
+centrifuge وبفصل، بعدين بقيس باستخدام meter
+
+467
+00:36:02,350 --> 00:36:08,410
+باستخدام مقياس مخصص للـ hematocrit بشوف نسبة ال...
+
+468
+00:36:08,410 --> 00:36:12,230
+ال...ال RBC's كام في المية من اللي هو المية في
+
+469
+00:36:12,230 --> 00:36:17,160
+المية العينة كلها تمام؟ هذا هو اللي راح يكون عنوان
+
+470
+00:36:17,160 --> 00:36:21,080
+محاضرتنا العملية اللي هي hematocrit تمام؟
+
+471
+00:36:21,080 --> 00:36:26,040
+طيب إذا وصلنا لنهاية محاضرتنا اليوم، أتمنى تكونوا
+
+472
+00:36:26,040 --> 00:36:29,540
+استفدتوا، يكون الشيء بسيط بالنسبة لكم، أي سؤال ما
+
+473
+00:36:29,540 --> 00:36:32,580
+تترددوا تسألوني إما بـ group ال whatsapp أو إن شاء
+
+474
+00:36:32,580 --> 00:36:38,000
+الله بالـ discussion الإسبوعي اللي إحنا موعدنا كل
+
+475
+00:36:38,000 --> 00:36:42,600
+ثابت الساعة ثمانية على google meet، وبتمنى لكم
+
+476
+00:36:42,600 --> 00:36:48,230
+التوفيق إن شاء الله، موعدنا يوم السبت الجاي على ال
+
+477
+00:36:48,230 --> 00:36:54,690
+google meet لحتى نتناقش بمحتوى المحاضرة، يعطيكم
+
+478
+00:36:54,690 --> 00:36:55,210
+العافية
diff --git a/PL9fwy3NUQKwY9jl8xw-bWkVZkWcGGyspd/FgXeSCpsx3M_raw.json b/PL9fwy3NUQKwY9jl8xw-bWkVZkWcGGyspd/FgXeSCpsx3M_raw.json
new file mode 100644
index 0000000000000000000000000000000000000000..5a586a51cc830e5eaf89ed3f8e28e212f88852ae
--- /dev/null
+++ b/PL9fwy3NUQKwY9jl8xw-bWkVZkWcGGyspd/FgXeSCpsx3M_raw.json
@@ -0,0 +1 @@
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220334, "start": 2173.62, "end": 2203.34, "text": "تمام؟ هذا هو اللي راح يكون عنوان محاضرتنا العملية اللي هي math of creative تمام؟ طيب إذا وصلنا لنهاية محاضرتنا اليوم أتمنى تكونوا استفدتوا يكون الأشي بسيط بالنسبالي لكم أي سؤال ما تترددوا تسألوني إما ب group ال whatsapp أو ان شاء الله بال discussion الإسبوعي اللي احنا موعدنا كل ثابت الساعة تماني على google meet و بتمنى لكم التوفيق", "tokens": [39237, 10943, 22807, 23758, 31439, 13672, 1829, 12602, 39319, 7251, 30544, 18871, 2407, 7649, 3714, 5016, 46958, 43500, 8315, 18863, 42213, 10632, 13672, 1829, 39896, 3803, 71, 295, 5880, 46811, 10943, 22807, 23032, 1829, 3555, 11933, 15730, 4032, 36520, 8315, 5296, 1863, 11296, 10632, 3714, 5016, 46958, 43500, 8315, 45595, 20498, 5551, 2655, 27842, 7578, 6055, 30544, 14407, 44713, 5172, 3215, 2655, 14407, 7251, 30544, 16247, 8592, 1829, 4724, 3794, 1829, 9566, 20666, 1863, 35457, 6027, 1829, 5296, 24793, 36632, 8608, 33604, 6027, 19446, 6055, 2655, 2288, 3215, 3215, 14407, 6055, 3794, 10721, 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2192.58, "end": 2192.78, "word": " الله", "probability": 0.94873046875}, {"start": 2192.78, "end": 2193.72, "word": " بال", "probability": 0.5576171875}, {"start": 2193.72, "end": 2195.3, "word": " discussion", "probability": 0.97412109375}, {"start": 2195.3, "end": 2196.14, "word": " الإسبوعي", "probability": 0.8631591796875}, {"start": 2196.14, "end": 2196.38, "word": " اللي", "probability": 0.9697265625}, {"start": 2196.38, "end": 2196.62, "word": " احنا", "probability": 0.8282063802083334}, {"start": 2196.62, "end": 2197.2, "word": " موعدنا", "probability": 0.85400390625}, {"start": 2197.2, "end": 2198.0, "word": " كل", "probability": 0.9853515625}, {"start": 2198.0, "end": 2198.4, "word": " ثابت", "probability": 0.7986653645833334}, {"start": 2198.4, "end": 2198.72, "word": " الساعة", "probability": 0.97802734375}, {"start": 2198.72, "end": 2199.1, "word": " تماني", "probability": 0.6394856770833334}, {"start": 2199.1, "end": 2199.3, "word": " على", "probability": 0.93115234375}, 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\ No newline at end of file
diff --git a/PL9fwy3NUQKwY9jl8xw-bWkVZkWcGGyspd/FgXeSCpsx3M_raw.srt b/PL9fwy3NUQKwY9jl8xw-bWkVZkWcGGyspd/FgXeSCpsx3M_raw.srt
new file mode 100644
index 0000000000000000000000000000000000000000..a5a0b9d2b121c5f69e625416e8abcd9c99e9c6b3
--- /dev/null
+++ b/PL9fwy3NUQKwY9jl8xw-bWkVZkWcGGyspd/FgXeSCpsx3M_raw.srt
@@ -0,0 +1,1912 @@
+1
+00:00:06,410 --> 00:00:10,510
+السلام عليكم و رحمة الله و بركاته طالبات العزيزات،
+
+2
+00:00:10,510 --> 00:00:14,410
+إذا ان شاء الله اليوم راح نبلش محاضرة جديدة بعنوان
+
+3
+00:00:14,410 --> 00:00:18,990
+centrifuges، أي أجهزة الطارد المركزي أو جهاز
+
+4
+00:00:18,990 --> 00:00:25,310
+الطارد المركزي، تمام؟ طيببس بداية خلونا نحكي عن
+
+5
+00:00:25,310 --> 00:00:30,350
+محاضرة ل blood component تمام؟ لما حكينا إنه عينة
+
+6
+00:00:30,350 --> 00:00:34,690
+الدم إذا أنا فصلتها باستخدام جهاز ال centrifuge
+
+7
+00:00:34,690 --> 00:00:39,270
+باستخدام جهاز الطارد المركزيال .. ال RBCs خلايا
+
+8
+00:00:39,270 --> 00:00:42,210
+ثقيلة الوزن راح تكون موجودة في ال sediment في
+
+9
+00:00:42,210 --> 00:00:47,610
+القاع تمام at the bottom و يليها طبق من ال .. ال
+
+10
+00:00:47,610 --> 00:00:51,290
+.. ال buffy coat اللي بتحتوي على ال white BCs و ال
+
+11
+00:00:51,290 --> 00:00:55,510
+blatelet خلايا الدم البيضاء و الصفائح الدموية و
+
+12
+00:00:55,510 --> 00:00:59,350
+راح يكون بعد هيك البلازمة اللي هى أخفقش في الوزن و
+
+13
+00:00:59,350 --> 00:01:04,910
+تكون at the top تمام ال RBCs راح تحتل نسبة خمسة و
+
+14
+00:01:04,910 --> 00:01:09,710
+..و أربعين في المية و ال .. و ال buffy coat أقل من
+
+15
+00:01:09,710 --> 00:01:15,010
+واحد في المية و ال .. ال .. ال plasma خمسة و خمسين
+
+16
+00:01:15,010 --> 00:01:21,450
+في المية تمام؟ طبعا في مراجع يعني ممكن تكون يذكروا
+
+17
+00:01:21,450 --> 00:01:25,450
+أن ال RBC بتحتل نسبة أربع و أربعين في المية و ال
+
+18
+00:01:25,450 --> 00:01:29,070
+buffy coat واحد في المية و ال .. ال plasma خمسة و
+
+19
+00:01:29,070 --> 00:01:33,400
+خمسين في المية يعني مجموحهم مية بالميةهلأ لو كانت
+
+20
+00:01:33,400 --> 00:01:36,420
+.. لو احنا .. لو انا حكيتلك ال RBC's خمسة و أربعين
+
+21
+00:01:36,420 --> 00:01:38,820
+في المية أو أربع و أربعين في المية مافيش خلاف
+
+22
+00:01:38,820 --> 00:01:43,060
+طبعا؟ لو كنت .. لو حكيت أنه ال RBC's خمسة و خمسين
+
+23
+00:01:43,060 --> 00:01:46,980
+في المية بكون بعتبر أنه ال .. ال .. ال buffy coat
+
+24
+00:01:46,980 --> 00:01:50,600
+negligable يعني مهمل أما إذا حكيتلك أنه أربع و
+
+25
+00:01:50,600 --> 00:01:52,920
+أربعين في المية بكون معتبرة أن ال buffy coat واحد
+
+26
+00:01:52,920 --> 00:01:57,710
+في المية طبعا؟ طيبالفكرة أنه باستخدام جهاز الطرد
+
+27
+00:01:57,710 --> 00:02:02,250
+المركزي بيتم فصل الأجزاء ل blood أو عينة الدم إلى
+
+28
+00:02:02,250 --> 00:02:07,130
+طبقة من الخلايا الثقيلة موجودة في القاع يليها طبقة
+
+29
+00:02:07,130 --> 00:02:11,830
+أخف وزنا موجودة اللي هي buffy coat و ثم الطبقة
+
+30
+00:02:11,830 --> 00:02:15,670
+الأخف على الإطلاق أو أخف واحدة فيهم هتكون موجودة
+
+31
+00:02:15,670 --> 00:02:21,670
+at the topإذاً هذا ما بفعل ال centrifuge يحدث بفعل
+
+32
+00:02:21,670 --> 00:02:25,850
+ال centrifuge باستخدام جهاز الطرد المركزي أو جهاز
+
+33
+00:02:25,850 --> 00:02:30,150
+ال fossil اللي احنا بنستخدمه بالمختبرات centrifuge
+
+34
+00:02:30,150 --> 00:02:34,990
+شو يعني centrifuge؟ هو عبارة عن equipment it's
+
+35
+00:02:34,990 --> 00:02:38,870
+equipment that is used to separate solid matter
+
+36
+00:02:38,870 --> 00:02:42,450
+from liquid suspension by mean of centrifugal
+
+37
+00:02:42,450 --> 00:02:46,790
+forcesأنا بفصل الأجزاء الصلبة أو الأجزاء الثقيلة
+
+38
+00:02:46,790 --> 00:02:51,770
+المعلقة داخل سائل بفعل أو بتأثير أو بأثر ال
+
+39
+00:02:51,770 --> 00:02:56,190
+centrifugal forces تمام؟ نفس الفكرة بالنسبة لل
+
+40
+00:02:56,190 --> 00:02:59,750
+blood ال blood هو عبارة عن خلايا عن أجزاء صلبة
+
+41
+00:02:59,750 --> 00:03:03,950
+خلايا صلبة تمام؟ معلقة في ال plasma اللي هي السائل
+
+42
+00:03:03,950 --> 00:03:08,680
+تمام؟ إذا solid matter هو الخلاياfrom يعني أنا بدي
+
+43
+00:03:08,680 --> 00:03:12,880
+أفصلها from liquid suspension من السائل اللي ..
+
+44
+00:03:12,880 --> 00:03:16,100
+اللي .. اللي معلقة فيه اللي هو عبارة عن ال plasma
+
+45
+00:03:16,100 --> 00:03:20,420
+بتأثير ال centrifugal forces ال sediment أو ال
+
+46
+00:03:20,420 --> 00:03:27,250
+solid matter ممكن يكون خلايا ممكن يكون بكتيرياcast
+
+47
+00:03:27,250 --> 00:03:32,690
+رواسب ممكن يكون parasite توفيليات وبيكون معلق
+
+48
+00:03:32,690 --> 00:03:37,510
+suspended in a fluid معلق فيه سائل لما انا بأثر
+
+49
+00:03:37,510 --> 00:03:42,570
+عليه بقوة ال centrifugal forces بقوة الطرد المركزي
+
+50
+00:03:42,570 --> 00:03:47,610
+انا ساعتها بفصله بحيث ان هذا ال sediment بيترسب
+
+51
+00:03:47,610 --> 00:03:52,490
+هذا ال ..ال solid matter بيترسب في قاعل tube و
+
+52
+00:03:52,490 --> 00:03:57,070
+السائل بيكون موجود على السطح، بينتقل إلى السطح،
+
+53
+00:03:57,070 --> 00:04:01,710
+تمام؟ هذه centrifugal forces هي عبارة عن force
+
+54
+00:04:01,710 --> 00:04:07,850
+greater than of the gravity، هي أضعاف قوة الجاذبية
+
+55
+00:04:07,850 --> 00:04:11,560
+الأرضية، هي قوة الطارد المركزيthe suspended
+
+56
+00:04:11,560 --> 00:04:17,540
+material المواد المعلقة في سائل are deposited in
+
+57
+00:04:17,540 --> 00:04:21,940
+the order of their weight بتترثبت بمعالجة وزنها
+
+58
+00:04:21,940 --> 00:04:27,440
+الأثقل على الإطلاق يكون في القاع و الأخف فيهم أخف
+
+59
+00:04:27,440 --> 00:04:32,540
+إشي بيكون موجود at the top تمام؟ و احنا حكينا مش
+
+60
+00:04:32,540 --> 00:04:38,260
+whyإنه في حالة مثلا عينة blood ال RBCs بتكون
+
+61
+00:04:38,260 --> 00:04:44,080
+موجودة at the bottom لإنها أثقل إشي يليها أخف وزنا
+
+62
+00:04:44,080 --> 00:04:47,920
+منها طبقة ال white BCs و البليلت و يليها الطبقة
+
+63
+00:04:47,920 --> 00:04:51,240
+الأخف إشي على الإطلاق اللي هي البلازمة في العينة
+
+64
+00:04:51,240 --> 00:04:55,580
+دي فبالتالي بتكون هي موجودة على ال top تمام أو على
+
+65
+00:04:55,580 --> 00:04:59,400
+ال surface على السطح تمام إذا the suspended
+
+66
+00:04:59,400 --> 00:05:03,680
+material الأجزاء المعلقة أو الجزيئات المعلقةبترتب
+
+67
+00:05:03,680 --> 00:05:08,060
+حسب وزنها أفقل إشي في القاع و أخف إشي at the top
+
+68
+00:05:08,060 --> 00:05:12,760
+طبعا there are many types of centrifuges فى عندى
+
+69
+00:05:12,760 --> 00:05:17,060
+أكتر من نوع من ال centrifuges و لكن كلها تقريبا
+
+70
+00:05:17,060 --> 00:05:22,160
+لها نفس مبدأ العمل طبعا و هى بتستخدم أو توظف ال
+
+71
+00:05:22,160 --> 00:05:25,720
+centrifugal forces اللى هى قوة الطرد المركزى فى
+
+72
+00:05:25,720 --> 00:05:29,180
+فصل الجزيعة طب خلونا نشوف مع بعض شو يعني
+
+73
+00:05:29,180 --> 00:05:34,610
+centrifugal forces و كيف هى الأجهزةتطبق مبدأ الـ
+
+74
+00:05:34,610 --> 00:05:39,150
+centrifugal forces لحتى تفصل المواد طيب تمام when
+
+75
+00:05:39,150 --> 00:05:43,570
+a body is rotated in a circular movement at speed
+
+76
+00:05:43,570 --> 00:05:48,130
+centrifugal force is created that drive the body
+
+77
+00:05:48,130 --> 00:05:51,390
+away from the center of the circular movement
+
+78
+00:05:51,390 --> 00:05:58,260
+تمام؟ هلأ لما بيكون في جسم body تمام؟بيتحرك حركة
+
+79
+00:05:58,260 --> 00:06:02,600
+دائرية circular movement at speed بسرعة معينة
+
+80
+00:06:02,600 --> 00:06:08,080
+هيكون بيأثر على هذا الجسم قوة اسمها centrifugal
+
+81
+00:06:08,080 --> 00:06:12,620
+force طبعا؟ بت .. هذا .. بت .. بت .. بت .. يعني
+
+82
+00:06:12,620 --> 00:06:17,580
+القوة هذه لها نزع بعيد عن المركز يعني بت .. بت ..
+
+83
+00:06:17,580 --> 00:06:23,400
+بتحرك هذا الجسم انه يروح بعيد عن المركزطبعا؟ إيش
+
+84
+00:06:23,400 --> 00:06:28,080
+يعني؟ هل في في الطبيعة أو في حوالنا إشي يحاول
+
+85
+00:06:28,080 --> 00:06:32,580
+يوضحلنا معنى الكلام هذا؟ أه فيه أكيد كلنا روحنا
+
+86
+00:06:32,580 --> 00:06:37,320
+على مدينة الملاهي و كلنا لعبنا بلعبة السلاسل طبعا؟
+
+87
+00:06:37,320 --> 00:06:43,820
+لعبة السلاسل هي عبارة عن إيش؟ هي عبارة عن زي عمود
+
+88
+00:06:43,820 --> 00:06:51,570
+أو ..محور مركزي تمام؟ و هذا المحور المركزي مرتبط
+
+89
+00:06:51,570 --> 00:06:56,550
+فيه عدة كراسي، هذا الكراسي هي ال body تمام؟ هي ال
+
+90
+00:06:56,550 --> 00:07:01,990
+body تمام؟ لما بت .. بنشغل لعبة ال .. السلاسلو ال
+
+91
+00:07:01,990 --> 00:07:06,670
+body هذا بيبدأ يتحرك ب circular movement، بيبدأ
+
+92
+00:07:06,670 --> 00:07:10,690
+يصير في حركة دائرية وسرعة لهذه الكراسي، ساعتها إيش
+
+93
+00:07:10,690 --> 00:07:14,290
+بيصير؟ بنلاحظ إن هي الكراسي هذه بعدت عن المركز،
+
+94
+00:07:14,290 --> 00:07:17,550
+صح؟ بتصير بعيدة، بتصير طايرة في الهواء، تمام؟
+
+95
+00:07:17,550 --> 00:07:22,420
+نتيجة بعضها عن المركز، ما الذي أبعدها عن المركز؟هي
+
+96
+00:07:22,420 --> 00:07:26,700
+ال centrifugal forces القوة اللي طردتها من المركز،
+
+97
+00:07:26,700 --> 00:07:34,240
+قوة طرد المركزي، تمام؟ نفس الاشي بيحدث في الجهاز
+
+98
+00:07:34,240 --> 00:07:38,360
+ال centrifuge أنا بحط ال body اللي هي الأنابيب،
+
+99
+00:07:38,360 --> 00:07:43,880
+أنابيب العيناتو بتتحرك بحركة دائرية مع سرعة
+
+100
+00:07:43,880 --> 00:07:47,620
+فبالتالي هذه الأنابيب إيش مالها؟ بتفرد، بتصير
+
+101
+00:07:47,620 --> 00:07:51,960
+بعيدة، بتصير بعيدة عن المركز، منتشرة، تمام؟
+
+102
+00:07:51,960 --> 00:07:59,370
+فبالتالي بيفصل محتوها بحيث إنه إيش؟الأسقل إشي
+
+103
+00:07:59,370 --> 00:08:03,310
+بيكون .. بيروح على .. بيروح أبعد ما يمكن عن المركز
+
+104
+00:08:03,310 --> 00:08:07,630
+و أخاف إشي بيكون أقرب ما يمكن من المركز إش معنى
+
+105
+00:08:07,630 --> 00:08:13,510
+هذا الكلام؟ يعني أبعد إشي عن المركز هو أسقل جزء في
+
+106
+00:08:13,510 --> 00:08:17,070
+العينة، يعني بيكون at the bottom of a tube، في
+
+107
+00:08:17,070 --> 00:08:23,970
+قاعلة tubeطبعا، ولكن الجزء اللي بيكون خفيف، light،
+
+108
+00:08:23,970 --> 00:08:29,750
+وزنه خفيف، بيكون قريب من المركز، يعني بيكون at the
+
+109
+00:08:29,750 --> 00:08:33,890
+top of التيوب، على سطح التيوب أو في السطح العلوي
+
+110
+00:08:33,890 --> 00:08:38,960
+من التيوبطبعا هاي فكرة عمل ال .. ال centrifuge وهي
+
+111
+00:08:38,960 --> 00:08:43,460
+معناه كلمة centrifugal forces قوة بتطرد ال objects
+
+112
+00:08:43,460 --> 00:08:48,920
+بتطرد ال body بعيدا عن المركز أثناء دورانه بحركة
+
+113
+00:08:48,920 --> 00:08:53,930
+دائرية على سرعة معينة، طبعا؟the greater the
+
+114
+00:08:53,930 --> 00:08:57,890
+outward pull due to rotation that is centrifugal
+
+115
+00:08:57,890 --> 00:09:01,730
+force, the more rapid and effective is the
+
+116
+00:09:01,730 --> 00:09:06,090
+sedimentation طبعا كل مكان فيه سحب أو بعد أو طرد
+
+117
+00:09:06,090 --> 00:09:10,830
+بعيد عن المركز، طبعا؟ معناه بيكون في عندي أكثر
+
+118
+00:09:10,830 --> 00:09:16,210
+سرعة و أكثر فعالية للفصل، لل sedimentation، لترسيب
+
+119
+00:09:16,210 --> 00:09:21,930
+الأجزاء الثقيلة، طبعا؟ طيبas a result فبالتالي
+
+120
+00:09:21,930 --> 00:09:27,370
+وينتج عن ذلك heavier elements are thrown to the
+
+121
+00:09:27,370 --> 00:09:32,470
+bottom of the tube الأجزاء السقيلة بتكون أبعد ما
+
+122
+00:09:32,470 --> 00:09:36,930
+يمكن عن المركز يعني بتكون في the bottom of ال tube
+
+123
+00:09:36,930 --> 00:09:40,290
+في قاعل ال tube لإن قاعل ال tube هو بيكون أبعد جزء
+
+124
+00:09:40,290 --> 00:09:44,830
+عن المركز تمام? followed by lighter particle و
+
+125
+00:09:44,830 --> 00:09:49,530
+بعدها بيليها الأجزاء الخفيفة اللي بتكونat the top
+
+126
+00:09:49,530 --> 00:09:52,670
+of the tube يعني بتكون قريبة نوعا ما من المركز
+
+127
+00:09:52,670 --> 00:09:57,750
+علشان نفهم الكلام اللي حكيناه خلونا نشوف صورة ال
+
+128
+00:09:57,750 --> 00:10:01,830
+centrifuge و نعرف كيف بيشتغل هاي ال centrifuge و
+
+129
+00:10:01,830 --> 00:10:05,370
+موجود فينا فرض موجود حطينا فيه أنبيض هذا ال
+
+130
+00:10:05,370 --> 00:10:10,630
+centrifuge و شغلناه صار يتحرك حركة دائرية على سرعة
+
+131
+00:10:10,630 --> 00:10:14,260
+معينةراح يأثر على القوة طارد مركزي، إيش يعني؟ يعني
+
+132
+00:10:14,260 --> 00:10:17,920
+الأنابيب هذه إيش معلها؟ راح تبعد عن المركز، إيش
+
+133
+00:10:17,920 --> 00:10:20,620
+يعني راح تبعد عن المركز؟ يعني البطم تبعها راح يبعد
+
+134
+00:10:20,620 --> 00:10:25,800
+عن المركز، راح تتحرك بعيد عن المركز، وهذه الأنابيب
+
+135
+00:10:25,800 --> 00:10:29,940
+إذا البطم تبعها بيكون بعيد عن المركز، طبعا؟ طيب
+
+136
+00:10:30,900 --> 00:10:34,240
+تبعًا لـ centrifugal force as a result
+
+137
+00:11:00,990 --> 00:11:06,270
+تمام؟ هى معناه انه انا افصل عينة باستخدام ال
+
+138
+00:11:06,270 --> 00:11:09,550
+centrifuge و تطبيق ال centrifugal forces اللى هى
+
+139
+00:11:09,550 --> 00:11:15,710
+قوة الطرد المركزى، تمام؟ طيب centrifugal forces
+
+140
+00:11:15,710 --> 00:11:19,870
+increase with the speed of rotation that is
+
+141
+00:11:19,870 --> 00:11:23,290
+revolution of the rotor per minute and the radius
+
+142
+00:11:23,290 --> 00:11:28,560
+of rotation هلأ قوة الطرد المركزى ب ..تزيد كل ما
+
+143
+00:11:28,560 --> 00:11:32,820
+زادت السرعة و السرعة بنعبر عنها revolution per
+
+144
+00:11:32,820 --> 00:11:36,240
+minute أو round per minute، كام دقيقة .. كام لفة
+
+145
+00:11:36,240 --> 00:11:41,660
+بيلف الجهاز بالدقيقة الواحدة هي سرعته، تمام؟ و
+
+146
+00:11:41,660 --> 00:11:46,060
+أيضا قطر الجهاز لإنه إحنا كل ما كان قطره كبير يعني
+
+147
+00:11:46,060 --> 00:11:51,340
+فيه هيكون إيش؟ هيكون .. هيصير طرد بعيد عن المركز،
+
+148
+00:11:51,340 --> 00:11:55,920
+تمام؟و هيكون بتاع ليه قوة الطرد المركزي أكتر، لذا
+
+149
+00:11:55,920 --> 00:12:04,840
+قوة الطرد المركزي بتتأثر يا بنات ب ..بال .. السرعة
+
+150
+00:12:04,840 --> 00:12:11,360
+.. سرعة الجهاز و أيضا بالقطر، قطر الجهاز، تمام؟
+
+151
+00:12:11,360 --> 00:12:17,640
+هذا إذا بتتأثر بسرعة الجهاز و بقطر الجهاز، تمام؟
+
+152
+00:12:17,640 --> 00:12:24,660
+إذا هذا بالنسبة لمين لال ..الcentrifugal forces
+
+153
+00:12:24,660 --> 00:12:30,500
+the actual sedimentation achieved at a given speed
+
+154
+00:12:30,500 --> 00:12:34,120
+depends therefore on the radius of centrifusion
+
+155
+00:12:34,120 --> 00:12:40,360
+هلأ لو أنا بدي أثبت السرعة a given speed يعني سرعة
+
+156
+00:12:40,360 --> 00:12:44,560
+معينة معناها ال sedimentation هيعتمد على مين؟
+
+157
+00:12:44,560 --> 00:12:47,840
+هيعتمد على ال radius على القطريعني ال centrifugal
+
+158
+00:12:47,840 --> 00:12:51,180
+forces طالما هي بتعتمد على السرعة و ال radius إذا
+
+159
+00:12:51,180 --> 00:12:55,160
+السرعة ثابتة معناها بتعتمد على ال radius طبعا؟ طيب
+
+160
+00:12:55,160 --> 00:13:00,020
+إذا يعني إشي واضح و منطقي most techniques
+
+161
+00:13:00,020 --> 00:13:03,460
+requiring centrifugation will usually specify that
+
+162
+00:13:03,460 --> 00:13:07,080
+require a relative centrifugal force expressed in
+
+163
+00:13:07,080 --> 00:13:12,810
+gravityهلأ معظم التقنيات و التجارب و الفحوصات اللي
+
+164
+00:13:12,810 --> 00:13:17,370
+إحنا بنستخدمها لابد إنه نمر بمرحلة فصل للعينة عشان
+
+165
+00:13:17,370 --> 00:13:21,370
+نحصل على مثلا plasma نحصل على serum إذا كان أنا
+
+166
+00:13:21,370 --> 00:13:25,550
+بستخدم blood أو نحصل على sediment إذا أنا بستخدم
+
+167
+00:13:25,550 --> 00:13:30,490
+عينة urine أو stool أو whatever تمام؟ لازم أنا قبل
+
+168
+00:13:30,490 --> 00:13:34,550
+ما أجري الفحص أستخدم إيش؟ أستخدم ال ..
+
+169
+00:13:37,540 --> 00:13:41,440
+يعني قبل لأجر الفحص أستخدم الcentrifugal حتى أفصل
+
+170
+00:13:41,440 --> 00:13:48,140
+العينة تبعتي طبعا طيب التجربة تبعتي بيكون مانشنلي
+
+171
+00:13:48,140 --> 00:13:52,640
+او محددلي انه افصلي على سرعة كذا او باستخدام
+
+172
+00:13:52,640 --> 00:13:57,660
+relative centrifugal forces قيمتها كذا طبعاو دايما
+
+173
+00:13:57,660 --> 00:14:00,660
+بيعبّر عن ال relative centrifugal forces على إنها
+
+174
+00:14:00,660 --> 00:14:05,560
+هي بدلالة ال G احنا حاكينا هي أضعف أضعف ل gravity،
+
+175
+00:14:05,560 --> 00:14:08,520
+لذا هيعبّر عنها بدلالة ال gravity، زي هنا مثال
+
+176
+00:14:08,520 --> 00:14:13,880
+بيحكي 2000 X gravity، يعني إن ال relative
+
+177
+00:14:13,880 --> 00:14:18,620
+centrifugal forcesهي تقريبا ألفين ضعف أو ألفين مرة
+
+178
+00:14:18,620 --> 00:14:23,340
+جد ل gravity طبعا ألفين ضعف ل gravity قوة الجاذبية
+
+179
+00:14:23,340 --> 00:14:28,320
+طبعا وهي هنا بنعبر عنها بقانون عبارة عن حاصل ضرب
+
+180
+00:14:28,320 --> 00:14:33,640
+ثابت واحد point واحد اتنين في عشرة أسالب خمسة في
+
+181
+00:14:33,640 --> 00:14:37,550
+الار في ال round per minute استربيةطبعا ال round
+
+182
+00:14:37,550 --> 00:14:39,790
+per minute او ال revolution per minute اللي هي
+
+183
+00:14:39,790 --> 00:14:42,650
+السرعة حكينا ان ال relative centrifuge forces
+
+184
+00:14:42,650 --> 00:14:46,630
+هتتعد تعتمد على ال R اللي هو ال radius و تعتمد على
+
+185
+00:14:46,630 --> 00:14:52,660
+السرعة ال speedتمام؟ هذا الكلام هو بس شرح
+
+186
+00:14:52,660 --> 00:14:56,360
+للاختصارات إيش يعني RCF؟ centrifugal forces أو
+
+187
+00:14:56,360 --> 00:15:00,320
+relative centrifugal forces قوة الطرد المركزي ال R
+
+188
+00:15:00,320 --> 00:15:03,840
+هو ال radius و ال round per minute أو ال
+
+189
+00:15:03,840 --> 00:15:06,740
+revolution per minute اللي هي السرعة و ال G
+
+190
+00:15:06,740 --> 00:15:11,000
+gravitational force طبعاً ده الوحدة اللي بنقيس
+
+191
+00:15:11,000 --> 00:15:16,700
+فيها إذا هذا الرقم بطلعلي مثلا لو أنا حسبت عوضت
+
+192
+00:15:16,700 --> 00:15:23,750
+بقيمة ال Rقطر الجهاز وسرعته وطلع عندي قيمة، هذه
+
+193
+00:15:23,750 --> 00:15:27,730
+القيمة عبارة هي عن .. مثلا طلعت خمس تلاف، هي خمس
+
+194
+00:15:27,730 --> 00:15:32,690
+تلاف G بكتب، يعني خمس تلاف مرة ضعف ال gravity اللي
+
+195
+00:15:32,690 --> 00:15:39,270
+هو الجاذبية الأرضية، تمام؟طيب نشوف إيش هي مكونات
+
+196
+00:15:39,270 --> 00:15:44,610
+هذا الجهاز لو أنا جيت أشوف إيش مكونات الجهاز يعني
+
+197
+00:15:44,610 --> 00:15:48,610
+مكوّن من ال central shaft المكوين الأول central
+
+198
+00:15:48,610 --> 00:15:53,310
+shaft، تمام؟ ال central shaft هو عبارة عن المحور
+
+199
+00:15:53,310 --> 00:16:00,280
+المركزي اللي موجود في منتصف الجهاز، تمام؟ و ..هو
+
+200
+00:16:00,280 --> 00:16:03,440
+اللي مسؤول عن الدوران هو اللي بيلف و بيدور طبعا
+
+201
+00:16:03,440 --> 00:16:09,960
+اذا هو the part that rotates الجزء اللذي يدور طبعا
+
+202
+00:16:09,960 --> 00:16:13,860
+لما بيصير فيه عملية spinning او centrifugation او
+
+203
+00:16:13,860 --> 00:16:17,380
+sedimentation او fossil separation طبعا هذه كلها
+
+204
+00:16:17,380 --> 00:16:20,540
+مصطلحات نفس الاشي لما اقول انا عملت spinning
+
+205
+00:16:20,540 --> 00:16:24,940
+للعينة اعملت centrifugation اعملت sedimentation
+
+206
+00:16:25,180 --> 00:16:28,680
+عملت separation نفس المعنى يعني أنا عملت فصلتها
+
+207
+00:16:28,680 --> 00:16:33,440
+باستخدام جهاز ال centrifuge طبعا طيب إذا هو الجزء
+
+208
+00:16:33,440 --> 00:16:40,800
+اللي بي rotate بيلف طبعا طيبال .. ال head هي ال ..
+
+209
+00:16:40,800 --> 00:16:45,400
+الرأس يعني هذه الحلقة الدائرية اللي موجودة في ..
+
+210
+00:16:45,400 --> 00:16:50,600
+يعني at the top of centrifuge موجودة في .. يعني
+
+211
+00:16:50,600 --> 00:16:54,680
+على .. أعلى ال centrifuge، هاي الحلقة الدائرية،
+
+212
+00:16:54,680 --> 00:16:57,600
+تمام؟ زي ما تشتركي ان هذا ال course الدائري بسميه
+
+213
+00:16:57,600 --> 00:17:01,360
+headتمام؟ هذا الhead عبارة عن فتحات و الفتحات ها
+
+214
+00:17:01,360 --> 00:17:06,140
+دي موجود فيها buckets موجود فيها مش أنبيب يعني هي
+
+215
+00:17:06,140 --> 00:17:10,880
+.. يعني مصممة ع شكل الإنبوب و أنا ما بحط فيها
+
+216
+00:17:10,880 --> 00:17:15,000
+العينة مباشرة لأ أنا بحط داخلها ال tube و داخل ال
+
+217
+00:17:15,000 --> 00:17:19,600
+tube بيكون فيه العينة تمام؟ اذا موجود فيها ال
+
+218
+00:17:19,600 --> 00:17:25,790
+buckets اللي يعني اللي بتستقبل العينة تمام؟إذا الـ
+
+219
+00:17:25,790 --> 00:17:30,010
+head هي عبارة عن it's a part that hold the bucket
+
+220
+00:17:30,010 --> 00:17:35,450
+إذا هو الجزء اللي بيحمل أو بيثبت ال buckets، اللي
+
+221
+00:17:35,450 --> 00:17:42,650
+هي مكان وضع العينة، تمام؟وهو اللي بيوصل القنابيب
+
+222
+00:17:42,650 --> 00:17:46,050
+حلقة وصل القنابيب مع ال central shaft يعني
+
+223
+00:17:46,050 --> 00:17:50,030
+القنابيب إذا حطت وموجودة على ال head خلاص طالما
+
+224
+00:17:50,030 --> 00:17:53,630
+أنه لف ال .. ال .. ال central shaft إذا صار يدور
+
+225
+00:17:53,630 --> 00:17:57,650
+معناها القنابيب صارت تدور معاه، تمام؟ طيب الجزء
+
+226
+00:17:57,650 --> 00:18:00,470
+التالت اللي هي ال buckets أو ال tubes حكينا إنها
+
+227
+00:18:00,470 --> 00:18:06,050
+هذه قنابيب مصممة أو buckets مصممة لتستقبل قنابيب
+
+228
+00:18:06,050 --> 00:18:10,770
+العينة إذا إن هي عبارة عن a portion جزء تمام؟that
+
+229
+00:18:10,770 --> 00:18:15,010
+hold test tubes بتثبت أنا بيه بالفحص أو أنا بيه
+
+230
+00:18:15,010 --> 00:18:18,030
+باللي أنا بشتغل فيها اللي فيها عينتي to beast band
+
+231
+00:18:18,030 --> 00:18:22,130
+طبعا الجهاز ال centrifuge يا بنات بيكون طبعا هذا
+
+232
+00:18:22,130 --> 00:18:26,650
+الجزء أنا ما بشوفه ده يعني ما بيكون مرئي هنا يعني
+
+233
+00:18:26,650 --> 00:18:32,190
+زي يعملي زي كأنه نازعالجزء الأمامي من الجهاز عشان
+
+234
+00:18:32,190 --> 00:18:37,290
+يشوفها يعني هذه رسمة توضيحية لمحتويات الجهاز ولكن
+
+235
+00:18:37,290 --> 00:18:40,370
+انا لما بشوف .. بس بشوف ال .. ال .. يعني انا لما
+
+236
+00:18:40,370 --> 00:18:43,490
+بدي ألقي نظرة بلقي نظرة علوية على الجهاز من هنا
+
+237
+00:18:43,490 --> 00:18:48,970
+تمام من الفتحة تبعته بشوف بس ال head و ال .. ال
+
+238
+00:18:48,970 --> 00:18:52,150
+buckets تمام؟ مابشوفش ال shaft ال central shaft
+
+239
+00:18:52,150 --> 00:18:59,950
+مابشوفهتمام؟ إذا هذه هي مكونات الجهاز طيب نيجي
+
+240
+00:18:59,950 --> 00:19:03,650
+لأنواع ال .. ال centrifuge classification of
+
+241
+00:19:03,650 --> 00:19:07,730
+centrifuge تصنيف ال centrifuge أو أنواعه طبعا حسب
+
+242
+00:19:07,730 --> 00:19:12,810
+على .. على ماذا يدار هذا الجهاز أو كيف يتم إدارة
+
+243
+00:19:12,810 --> 00:19:18,190
+أو تشغيل هذا الجهاز يتصنف ال centrifuges ل hand
+
+244
+00:19:18,190 --> 00:19:22,980
+centrifuges و electrical centrifugesمن اسمها hand
+
+245
+00:19:22,980 --> 00:19:27,720
+centrifuges إذا .. إذا تدار باستخدام .. إيش؟
+
+246
+00:19:27,720 --> 00:19:31,780
+باستخدام اليد أما ال .. ال electrical centrifuges
+
+247
+00:19:31,780 --> 00:19:35,980
+تدار باستخدام الكهربا طبعا؟ طيب خلينا نشوف إيش
+
+248
+00:19:35,980 --> 00:19:40,080
+يعني hand centrifugesزي ما انتوا شايفين هي عبارة
+
+249
+00:19:40,080 --> 00:19:44,300
+عن old fashion of centrifuge يعني ال centrifuge
+
+250
+00:19:44,300 --> 00:19:51,060
+هيك موديل قديم، دقة قديمة، تمام؟ ويعني ال .. ال ..
+
+251
+00:19:51,060 --> 00:19:54,780
+ال .. موديل القديم من ال centrifuge ب .. ب .. بيتم
+
+252
+00:19:54,780 --> 00:19:57,980
+تشغيله باستخدام ال hand يعني باليدوي، تمام؟ انا
+
+253
+00:19:57,980 --> 00:20:03,290
+بحرك ال .. اليد هادي و بيصير يدورطمام؟ هى زى ما
+
+254
+00:20:03,290 --> 00:20:06,370
+انتوا شايفين الـ central shaft و head هى الدائرة
+
+255
+00:20:06,370 --> 00:20:09,970
+اللى عاملة زى ال cross و ال buckets هى هى طبعا
+
+256
+00:20:09,970 --> 00:20:14,150
+هادى انا بحط فيها القنابيب تبعتي طيب اذا بيتم
+
+257
+00:20:14,150 --> 00:20:17,490
+تشغيله اما بال hand او بال water pressure يعني
+
+258
+00:20:17,490 --> 00:20:20,950
+ممكن ضغط ماء يأثر على اليد هادى و تصير يحركها
+
+259
+00:20:20,950 --> 00:20:25,570
+فاتلف و يلف معاها الجهاز و يتم الفصلطبعا بما ان
+
+260
+00:20:25,570 --> 00:20:28,610
+old fashion معناها بيستخدم في ال small
+
+261
+00:20:28,610 --> 00:20:34,170
+laboratories كان يستخدم قديما بيستخدم ممكن في ال
+
+262
+00:20:34,170 --> 00:20:37,870
+routine purposes اش يعني routine purposes؟ لما
+
+263
+00:20:37,870 --> 00:20:44,510
+احكي routine test او routine lab work يعني urine
+
+264
+00:20:44,510 --> 00:20:50,250
+and stool analysis طبعا؟ اذا بيستخدم هذا النوع من
+
+265
+00:20:51,010 --> 00:20:55,410
+ال .. ال .. ال centrifuges في ال routine work يعني
+
+266
+00:20:55,410 --> 00:20:58,810
+في ال urinary sediment عشان أحضر ال urinary
+
+267
+00:20:58,810 --> 00:21:02,210
+sediment أفصل عينة ال urine عشان أعمل
+
+268
+00:21:02,210 --> 00:21:05,110
+concentration لل parasites اللي موجود في ال stool
+
+269
+00:21:05,110 --> 00:21:08,730
+تمام؟ إذا عينات ال urine هو ال stool اللي بيستخدم
+
+270
+00:21:08,730 --> 00:21:15,030
+it is not advisable not advisable مش يعني غير موصف
+
+271
+00:21:15,030 --> 00:21:19,340
+أو غير .. لا ينصحبإنه هذا الجهاز يستخدم عشان أفصل
+
+272
+00:21:19,340 --> 00:21:25,260
+عينة دم فيه، ليش؟ لإنه أنا بستخدم يدوي وحركة يدي
+
+273
+00:21:25,260 --> 00:21:28,820
+مش منتظمة، إذا ال relative centrifugal forces
+
+274
+00:21:28,820 --> 00:21:33,220
+هتكون irregular طالما إنه كانت القوة irregular
+
+275
+00:21:33,220 --> 00:21:37,660
+معناها عملية الفصل غير منتظمة، يعني ممكن ال RBCs و
+
+276
+00:21:37,660 --> 00:21:41,860
+هي عم تنفصل و هي عم بتتجه باتجاه ال sediment تتكسر
+
+277
+00:21:42,510 --> 00:21:45,690
+بالتالي ال serum أو ال plasma اللي أنا بدي أخدها
+
+278
+00:21:45,690 --> 00:21:50,810
+بدل ما يكون لونها لون أصفر و قشي strong color و
+
+279
+00:21:50,810 --> 00:21:56,570
+تكون clear ممكن تتحول لونها للون الأحمر نتيجة تكسر
+
+280
+00:21:56,570 --> 00:22:02,350
+ال RBCs و أيضا خروج محتويات ال RBCs على ال serum
+
+281
+00:22:02,350 --> 00:22:06,710
+أو على ال plasma ممكن يؤثر على عدة فحوصات و تصبح
+
+282
+00:22:06,710 --> 00:22:11,870
+العينة غير صالحة لإجراء الفحوصاتتمام؟ فبالتالي إذا
+
+283
+00:22:11,870 --> 00:22:16,110
+لا ينصح بيه لفصل عينة قدام خلينا نشوف إيش يعني
+
+284
+00:22:16,110 --> 00:22:20,450
+electrical centrifuges electrical centrifuges هي
+
+285
+00:22:20,450 --> 00:22:23,610
+عبارة عن ال centrifuges that are operated by
+
+286
+00:22:23,610 --> 00:22:27,890
+electrical power تمام؟ بيتم إدارتها باستخدام
+
+287
+00:22:27,890 --> 00:22:33,070
+الكهرباء and produce high centrifugal force ب ..
+
+288
+00:22:33,070 --> 00:22:41,000
+يعني ..بتنتج يعني قوة طارد مركزي عالية جدا و
+
+289
+00:22:41,000 --> 00:22:44,860
+بتستخدم في ال most medical laboratories و بتستخدم
+
+290
+00:22:44,860 --> 00:22:49,200
+ايضا لفصل عينات الدم يعني بتعطيني انه يعني فصل
+
+291
+00:22:49,200 --> 00:22:55,570
+دقيق و فصل يعني جيدتمام؟ هذا بالنسبة ليه .. how it
+
+292
+00:22:55,570 --> 00:22:59,870
+operated كيف أنا ممكن أشغّل الجهاز by hand or by
+
+293
+00:22:59,870 --> 00:23:04,590
+electric .. electric أو electricity تمام؟ طيب، هلأ
+
+294
+00:23:04,590 --> 00:23:10,470
+بالنسبة لحجم ال centrifuge، في عندي أنواع منهفي
+
+295
+00:23:10,470 --> 00:23:14,310
+micro centrifuge يعني بيستخدم أنا بيب صغيرة أو
+
+296
+00:23:14,310 --> 00:23:18,490
+بسميه zero centrifuge هذا يا بنات بيفصل بينستخدمه
+
+297
+00:23:18,490 --> 00:23:22,750
+for spinning لفصل small tubes يعني انا العينات
+
+298
+00:23:22,750 --> 00:23:25,410
+تبعتي بتكون موجودة في ال small tubes زي ال
+
+299
+00:23:25,410 --> 00:23:28,570
+centrifuges اللي بتكون موجودة في ال blood bank
+
+300
+00:23:28,570 --> 00:23:32,990
+laboratories في مختبرات اللي هو بنوك الدم طبعا
+
+301
+00:23:32,990 --> 00:23:39,100
+خلينا نشوف إيش يعني ال .. ال ..ال small tubes هلأ
+
+302
+00:23:39,100 --> 00:23:42,520
+في عندي tube بسميها ebendrof tube هي صغيرة كتير
+
+303
+00:23:42,520 --> 00:23:45,480
+يعني انها بتاخد بس واحد صندي واحد امقال او واحد و
+
+304
+00:23:45,480 --> 00:23:49,940
+نص امقال تمام هي زي ما انتوا شايفين هنا هي مدرجة و
+
+305
+00:23:49,940 --> 00:23:55,040
+بتاخد يعني واحد امقال او واحد و نص امقال فصغيرة
+
+306
+00:23:55,040 --> 00:23:59,220
+كتير محتوها صغير العينة تبعت اللي هتكون فيها small
+
+307
+00:23:59,220 --> 00:24:04,250
+amountفبالتالي هي small tubes فالجهاز تبعي ال
+
+308
+00:24:04,250 --> 00:24:08,530
+centrifuge بسمي اللي بيفصل او بيستقبل هاي القنابيب
+
+309
+00:24:08,530 --> 00:24:12,110
+و بفصلها بسمي micro centrifuge او sericentrifuge
+
+310
+00:24:12,110 --> 00:24:14,310
+معناها هذا ال micro centrifuge او ال
+
+311
+00:24:14,310 --> 00:24:17,190
+sericentrifuge ال bucket تبعته هتكون صغيرة مصممة
+
+312
+00:24:17,190 --> 00:24:23,040
+على جد عشان ال .. ال .. ال ependrofetubeتمام؟ طيب
+
+313
+00:24:23,040 --> 00:24:27,660
+ال .. بالنسبة ل ال medium-sized centrifuge حجم
+
+314
+00:24:27,660 --> 00:24:31,000
+متوسط يعني بنستخدمه لفصل عينات ال urine ممكن
+
+315
+00:24:31,000 --> 00:24:35,420
+لعينات ال blood بيستخدم medium-sized tubes بيفصل
+
+316
+00:24:35,420 --> 00:24:38,080
+medium-sized tubes اللي هي بنسميها centrifuge
+
+317
+00:24:38,080 --> 00:24:43,120
+tubes اللي أنا بتبعته بسميها centrifuge tubesزي
+
+318
+00:24:43,120 --> 00:24:47,900
+كيف شكلها ممكن يكون هيك شكلها مثلا هذه ممكن أسميها
+
+319
+00:24:47,900 --> 00:24:52,200
+هي falcon tube ويلها غطاية هي اسمها falcon tube
+
+320
+00:24:52,200 --> 00:24:56,740
+تمام ويلها غطاية و لكن هي centrifuge tube نحطها في
+
+321
+00:24:56,740 --> 00:25:00,240
+ال centrifuge و بتفصل تمام و لكن مش هي الأكتر
+
+322
+00:25:00,240 --> 00:25:03,720
+شيوعا في عندي نوع من القنابيب أكتر شيوعا زيها بس
+
+323
+00:25:03,720 --> 00:25:08,020
+مايله غطاية هلأ بورجيكميا هي تمام
+
+324
+00:25:12,270 --> 00:25:18,370
+وينو كان موجود من شوي هنا هاي .. هذا بسميه
+
+325
+00:25:18,370 --> 00:25:22,470
+centrifuge tube تمام؟ و بنستخدم .. و هنستخدمه كتير
+
+326
+00:25:22,470 --> 00:25:28,850
+في المختبرات طبعتنا medium size تمام؟ طيب لكن ال
+
+327
+00:25:28,850 --> 00:25:33,110
+.. ال .. ال large centrifuges ال .. ال centrifuges
+
+328
+00:25:33,110 --> 00:25:36,290
+الكبيرة بتاخد أنابيب كتير كبيرة يا بنات يعني هاي
+
+329
+00:25:36,290 --> 00:25:42,940
+مثلا ممكن زي هاي الأنابيبشايفين هنا الحجم تبعها
+
+330
+00:25:42,940 --> 00:25:50,280
+بيصل لخمسين أمقال يعني، تمام؟ هذا هو الحجم اللي
+
+331
+00:25:50,280 --> 00:25:54,640
+أنا بيبلي كبيرة، ممكن يعني بياخد لخمسين أمقال،
+
+332
+00:25:54,640 --> 00:25:59,500
+ممكن فلسكات، تيوبات حجمها كبير، فبالتالي هذه
+
+333
+00:25:59,500 --> 00:26:03,080
+بسميها إيش؟ Large Centrifugesنستخدمها في ال
+
+334
+00:26:03,080 --> 00:26:07,640
+bacteriology أو أحيانا كمان في ال cytology في ال
+
+335
+00:26:07,640 --> 00:26:15,420
+.. ال .. لعبات الخلايا و ال .. يعني علم الخلايا و
+
+336
+00:26:15,420 --> 00:26:18,900
+العلم ال micro part لو أنا مثلا عاملة enrichment
+
+337
+00:26:18,900 --> 00:26:22,800
+عاملة cultivation .. culture لبكتيريا، زراعة
+
+338
+00:26:22,800 --> 00:26:27,340
+بكتيريا بكميةبكميات يعني في .. في أنابيب كبيرة و
+
+339
+00:26:27,340 --> 00:26:30,160
+هلأ بدي أفصل ال .. أنا ال bacteria بحيث أني
+
+340
+00:26:30,160 --> 00:26:33,320
+أرسّبها و أستخدمها، أعمل عليها تطبيقات، بستخدم ال
+
+341
+00:26:33,320 --> 00:26:38,000
+dark centrifuge، تمام؟ طيب، هذا بالنسبة لمين؟ لـ
+
+342
+00:26:38,000 --> 00:26:43,160
+ال .. ال أنواع ال centrifuges حسب حجمها، تمام؟أي
+
+343
+00:26:43,160 --> 00:26:48,160
+جهاز أنا بدي أستخدمه طبعا لازم في عندي تعليمات كيف
+
+344
+00:26:48,160 --> 00:26:51,960
+أنا أستخدمه و كيف أنا أعتني بهذا الجهاز و أحافظ
+
+345
+00:26:51,960 --> 00:26:55,760
+عليه أول إشي reading of the manufacturer
+
+346
+00:26:55,760 --> 00:27:00,520
+instruction لما أنا أقرأ تعليمات الجهة المصنع لهذا
+
+347
+00:27:00,520 --> 00:27:05,280
+الجهاز الكتيب اللي بيجي مع الجهاز ساعتها أنا عرفت
+
+348
+00:27:05,280 --> 00:27:09,500
+كيف أستخدمه كيف أحافظ عليه كيف انا يعني المحاذير
+
+349
+00:27:09,500 --> 00:27:14,640
+اللي بتكون يعني مرفقة مع هذاالجهاز طبعا؟ طيب و
+
+350
+00:27:14,640 --> 00:27:21,560
+أيضا لازم كجهاز centrifuge انا اعمله placing على
+
+351
+00:27:21,560 --> 00:27:25,140
+firm level bench ايش يعني؟ يعني احطه على bench
+
+352
+00:27:25,140 --> 00:27:29,320
+ثابت غير .. يعني مش .. غير قبل الاهتزاز او الحركة
+
+353
+00:27:29,320 --> 00:27:35,760
+او انه ممكن الجهاز ييجع منه طبعا؟و أيضا لإنه يا
+
+354
+00:27:35,760 --> 00:27:41,300
+بنات لما إحنا بنشغل هذا الجهاز بيعطيني ذدابات أو
+
+355
+00:27:41,300 --> 00:27:46,320
+vibrations اهتزازات لو ماكانش على بنش ثابت و
+
+356
+00:27:46,320 --> 00:27:51,420
+الجهاز موجود يعني ثابت على ال .. ال bench فممكن
+
+357
+00:27:51,420 --> 00:27:56,160
+إنه يتحرك و يجعل تمام؟ و أيضا لازم أنا أبعده عن ال
+
+358
+00:27:56,160 --> 00:28:01,890
+sunlight عن ضوء الشمسيخرب و بحطه داخل ال bench
+
+359
+00:28:01,890 --> 00:28:05,630
+يعني مش على طرف ال bench لإنه مع الاهتزازات ممكن
+
+360
+00:28:05,630 --> 00:28:09,930
+أنه يقع الجهاز always closing the centrifuge
+
+361
+00:28:09,930 --> 00:28:15,010
+before turning it on ده طبعا لازم أن أنا أسكر
+
+362
+00:28:15,010 --> 00:28:18,990
+غطايت ال centrifuge لما بدي أشغل ال centrifuge
+
+363
+00:28:18,990 --> 00:28:24,220
+لازم أنا أسكر ال cover طبعه بعدين أشغلهليش؟ لإنه
+
+364
+00:28:24,220 --> 00:28:27,880
+لو أنا شغلته هو فاتح ممكن تطاير الأنابيب أو يطاير
+
+365
+00:28:27,880 --> 00:28:32,820
+محتوياها أو ينكب نتيجة فعل ال .. يعني ال rotation،
+
+366
+00:28:32,820 --> 00:28:37,900
+تمام؟ فبالتالي خطأ إن أنا أشغله هو فاتح لازم أسكر
+
+367
+00:28:37,900 --> 00:28:43,480
+الجهاز قبل لأشغله Always balancing the tubes that
+
+368
+00:28:43,480 --> 00:28:47,890
+are being centrifugedهلأ طبعا لازم انا اعمل
+
+369
+00:28:47,890 --> 00:28:52,270
+balance لليوبيات على مرجوحة ال .. ال .. السلاسل
+
+370
+00:28:52,270 --> 00:28:55,590
+لما بيشوا بي .. بيجلسوا الناس اللي جاية تلعب
+
+371
+00:28:55,590 --> 00:29:00,850
+بيجلسوهم كل اتنين مقابل بعضهم و بيكونوا طبعا نفس
+
+372
+00:29:00,850 --> 00:29:04,610
+الحجم أو نفس الوزن، يعني نفس الكتلة، تمام؟ يفضل،
+
+373
+00:29:04,610 --> 00:29:09,000
+يرعى، هيكطبعا فنفس الفكرة في جهاز ال centrifuge
+
+374
+00:29:09,000 --> 00:29:13,640
+لما انا بدي احط القنابيب داخل ال centrifuge لازم
+
+375
+00:29:13,640 --> 00:29:17,440
+احطها balance اش يعني كل tube مقابلها tube نفس
+
+376
+00:29:17,440 --> 00:29:21,800
+الشكل و نفس الحجم و نفس المحتوى طبعا بحطهم مقابل
+
+377
+00:29:21,800 --> 00:29:25,770
+بعضهم البعضطب إذا أنا ماعملت balancing راح يصير
+
+378
+00:29:25,770 --> 00:29:29,950
+فيه اهتزازات للجهاز ممكن يقع ممكن تتكسر الأنابيب
+
+379
+00:29:29,950 --> 00:29:35,110
+في الداخل أو ينكب محتوها، تمام؟ وبيعطي صوت يعني
+
+380
+00:29:35,110 --> 00:29:38,830
+إزعاج، بيبطل يستوعب الجهاز، نتيجة أنه مافيش
+
+381
+00:29:38,830 --> 00:29:44,890
+balance، تمام؟ فإذا جزئية ال balance أو نقطة ال
+
+382
+00:29:44,890 --> 00:29:48,630
+balance مهمة جدا يا بناتال tubes should also be
+
+383
+00:29:48,630 --> 00:29:51,230
+the same size and should contain the same amount
+
+384
+00:29:51,230 --> 00:29:53,610
+الأنابيب اللي أنا بدي أحطها بالمقابل بعضها البعض
+
+385
+00:29:53,610 --> 00:29:58,770
+لازم تكون نفس الحجم ومحتوها أيضا نفس الكمية طب أنا
+
+386
+00:29:58,770 --> 00:30:05,030
+عندي انبوب واحدة فيها 5 cm أو 5 أمقال من ال blood
+
+387
+00:30:05,030 --> 00:30:08,790
+طب أنا كيف بدي أفصلها كيف بدي أعمل لها balance؟
+
+388
+00:30:08,790 --> 00:30:12,590
+مالهاش balance أحطها لحالها؟ لأ يستحق أحطها لحالها
+
+389
+00:30:12,950 --> 00:30:17,830
+لازم أحط tube .. أجيب tube نفس الشكل و نفس الحجم و
+
+390
+00:30:17,830 --> 00:30:23,150
+أحط فيها خمسة أمقال water تمام? distilled water أو
+
+391
+00:30:23,150 --> 00:30:27,290
+water .. tap water تمام؟ و أحطهم مقابل بعضهم البعض
+
+392
+00:30:27,290 --> 00:30:33,150
+في الجهاز نهائيا الجهاز ما بيستوعب ولا بيشتغل إذا
+
+393
+00:30:33,150 --> 00:30:37,450
+ما .. انا ماحطيت balanceتمام؟ إذا ما حطيتله إشي
+
+394
+00:30:37,450 --> 00:30:41,770
+usingه يعني كل عينة مقابلها عينة أو كل tube
+
+395
+00:30:41,770 --> 00:30:45,730
+مقابلها tube نفس الشكل، نفس الحجم، نفس المحتوى أو
+
+396
+00:30:45,730 --> 00:30:50,660
+نفس الكمية من المحتوى، تمام؟ إذا أنا ما ..يعني
+
+397
+00:30:50,660 --> 00:30:54,000
+مابدى أحط balance الجهاز بتنح بيبقى بيصير ما
+
+398
+00:30:54,000 --> 00:30:57,620
+بيشتغل بيكسر القنابيب و بيكب القنابيب و بطلع صوت
+
+399
+00:30:57,620 --> 00:31:00,460
+كتير كبير عالي و بيصير فيه اهتزازات و vibration
+
+400
+00:31:00,460 --> 00:31:05,540
+يعني الجهاز بطل يستوعب عن جد تمام never open the
+
+401
+00:31:05,540 --> 00:31:09,240
+centrifuge while it is spinning never try to slow
+
+402
+00:31:09,240 --> 00:31:13,800
+it down with your hand طبعا ممنوع منعا باتا انه
+
+403
+00:31:13,800 --> 00:31:18,500
+احاول اوقف الجهاز أثناء دورانه او يعني او احاول
+
+404
+00:31:18,500 --> 00:31:23,890
+ابطق بإيديأول إشي ممنوع أفتح الجهاز أثناء دورانه
+
+405
+00:31:23,890 --> 00:31:27,170
+لإنه ممكن تطير الأنابيب و .. أو يكبل محتوى .. أو
+
+406
+00:31:27,170 --> 00:31:31,370
+يطير محتواها، تمام؟ هاي شغلة، نتيجة السرعة
+
+407
+00:31:31,370 --> 00:31:36,990
+العالية، لو إنتي على .. على لعبة سلاسل و إن فتح
+
+408
+00:31:36,990 --> 00:31:41,070
+الكرسي، بطل فيه أمان للكرسي، بتطير الشخص اللي
+
+409
+00:31:41,070 --> 00:31:44,690
+بيرعب على الكرسي، يعني جارس على اللعبة، تمام؟ نفس
+
+410
+00:31:44,690 --> 00:31:50,880
+الفكرة هناإذا أنا فتحت ال .. ال cover تبع الجهاز
+
+411
+00:31:50,880 --> 00:31:56,910
+بتطير الأنابيب أو محتوهاتمام؟ ممنوع لما ال ..
+
+412
+00:31:56,910 --> 00:32:01,870
+الجهاز بيتوقف عن الدوران أو بتخلص مدة دورانه لحاله
+
+413
+00:32:01,870 --> 00:32:06,890
+بيصير يبطء تدريجي لإلى أن يقف تمام؟ ممنوع أنا
+
+414
+00:32:06,890 --> 00:32:12,530
+أحاول أ .. أسرع او أباطئ حركته بإيدي، أسرع إيقافه
+
+415
+00:32:12,530 --> 00:32:16,690
+أو أبطئ حركته بإيدي، أعمله slow down بإيدي، بyour
+
+416
+00:32:16,690 --> 00:32:22,350
+handممنوع؟ ليش؟ طيب خلينا نشوف هنا هي عين الدم
+
+417
+00:32:22,350 --> 00:32:25,210
+انفصلت باستخدام جهاز ال centrifuge أفضل اشي ال
+
+418
+00:32:25,210 --> 00:32:28,090
+RBCs نزلت لتحت في ال bottom بعدها ال buffy coat
+
+419
+00:32:28,090 --> 00:32:34,050
+بعدها ال plasma طبعا لو أنا جيت أثناء الجهاز هو عم
+
+420
+00:32:34,050 --> 00:32:39,950
+بيوقف أو بيبطق سرعته لحتى يقف بعد ما خلص فصل طبعا
+
+421
+00:32:39,950 --> 00:32:43,010
+بعد ما صار الشكل اللي منضوب بهذا الشكل يعني خلص
+
+422
+00:32:43,010 --> 00:32:48,710
+فصل طبعالو أنا جيت حطيت إيدي عشان أسرع إيقافه، جزء
+
+423
+00:32:48,710 --> 00:32:52,350
+من ال red blood cell اللي هي ال sediment اللي في
+
+424
+00:32:52,350 --> 00:32:56,570
+القاع بيندفع باتجاه ال .. ال .. الطب، باتجاه ال
+
+425
+00:32:56,570 --> 00:33:00,450
+surface، تمام؟ يعني بتختلط الأنبوبة مرة تانية،
+
+426
+00:33:00,450 --> 00:33:04,530
+أصبح ساعتها ال sedimentationال separation ال
+
+427
+00:33:04,530 --> 00:33:09,470
+centrifugation ال spinning اشماله not efficient مش
+
+428
+00:33:09,470 --> 00:33:13,730
+فعال مش كويس انا مافصلتش كويس لإنه انا بال .. يعني
+
+429
+00:33:13,730 --> 00:33:22,230
+بالاخر يعني .. فصلت العينة بالاخر انا جيت خربتها
+
+430
+00:33:22,230 --> 00:33:25,710
+بنفسي لما حطيت إيدي على الجهاز عشان أسرع إيقافه
+
+431
+00:33:25,710 --> 00:33:27,270
+طبعا؟ طيب
+
+432
+00:33:29,630 --> 00:33:34,370
+إذا بستناله لحتى يتوقف بنفسه ما بوقفه بإيدي أو
+
+433
+00:33:34,370 --> 00:33:38,350
+بحاول أبطق سرعته بإيدي لو في حال .. لو في حال أنا
+
+434
+00:33:38,350 --> 00:33:42,810
+مثلا انسيت أن أنا أعمل balance انسيت أضيف tube أو
+
+435
+00:33:42,810 --> 00:33:46,980
+أحط بعض القنابيب يعنيحطت جزء منهم و ماحطت الجزء
+
+436
+00:33:46,980 --> 00:33:50,560
+التاني فممكن في عندي حاجة اسمها break في الجهاز
+
+437
+00:33:50,560 --> 00:33:54,760
+بيوقفوا بسرعة بحيث أن أنا أفتح الجهاز و أحط
+
+438
+00:33:54,760 --> 00:33:59,920
+قنابيبي يعني القنابيب اللي بدي أحطهم و بعدين أرجع
+
+439
+00:33:59,920 --> 00:34:05,820
+أسكر الجهاز و يرجع الجهاز ي .. يعمل around أو
+
+440
+00:34:05,820 --> 00:34:11,380
+centrifugal forces أو ..rotation مرة تانية، طبعا،
+
+441
+00:34:11,380 --> 00:34:14,300
+إذا ال break أنا مابستخدمه إلا إذا في حالة طارقة،
+
+442
+00:34:14,300 --> 00:34:17,880
+أنا بدي وقفه بحق .. بدي أوقف الجهاز في حالة طارقة،
+
+443
+00:34:17,880 --> 00:34:21,680
+طبعا، ولكن أنا إذا بدي أشتغل، بدي أفصل منيح، يفضل
+
+444
+00:34:21,680 --> 00:34:25,080
+إن أنا أستنى على الجهاز لحتى يتباطأ سرعته تدريجيا
+
+445
+00:34:25,080 --> 00:34:30,440
+ويقف لحالهطبعا في timer في الجهاز انا بحدد السرعة
+
+446
+00:34:30,440 --> 00:34:35,040
+اللي بدياها هاي هنا و بحدد ال timer يعني كام وقت
+
+447
+00:34:35,040 --> 00:34:38,200
+الفصل five minutes بعد الجهاز عن five minutes بس
+
+448
+00:34:38,200 --> 00:34:44,460
+يخلصه بيبدأ .. بي .. بيعطيني run هذا الجرس تبعه
+
+449
+00:34:44,460 --> 00:34:47,760
+بيعطيني run انه خلاص توقف الفصل و توقف الجهاز و
+
+450
+00:34:47,760 --> 00:34:53,580
+خلصت المدة فبتبدأ تتباطأ حركته الى ان يقف لحاله
+
+451
+00:34:53,580 --> 00:34:58,850
+طبعا؟هذا بالنسبة لجهاز ال centrifuge ان شاء الله
+
+452
+00:34:58,850 --> 00:35:03,290
+كتطبيق على جهاز ال centrifuge راح ناخد فحص اسمه
+
+453
+00:35:03,290 --> 00:35:07,830
+hematocrit تمام؟ او نسبة ال RBC's نسبة .. نسبة
+
+454
+00:35:07,830 --> 00:35:11,270
+المئوية ل ال RBC's من ايش؟ من ال whole blood
+
+455
+00:35:11,270 --> 00:35:15,750
+volume يعني جدش بتحتل ال RBC's من ال whole volume
+
+456
+00:35:15,750 --> 00:35:19,730
+ده اعتبر ان العينة ال blood كلها 100% و اشوف ال
+
+457
+00:35:19,730 --> 00:35:24,170
+RBC's كام في المية من هذه العينةتمام؟ احنا أخدنا
+
+458
+00:35:24,170 --> 00:35:28,150
+إنه هي theoretical 45% بنشوف عند .. في ال .. في
+
+459
+00:35:28,150 --> 00:35:31,890
+البناء أدمين كام بتكون نسبتها أو يعني كام ال range
+
+460
+00:35:31,890 --> 00:35:38,060
+طبعهاتمام هذا ال .. التطبيق العملي رح يكون يعني
+
+461
+00:35:38,060 --> 00:35:42,100
+أنا راح أسحب عين الدم راح أحطها في tube طبعا ال ..
+
+462
+00:35:42,100 --> 00:35:46,060
+ال .. ال .. هذا شرح عشان ي .. يقربلي الفكرة ولكن
+
+463
+00:35:46,060 --> 00:35:49,820
+أنه أنا بدي أحطها في tube خاصة في الفحص ال
+
+464
+00:35:49,820 --> 00:35:53,500
+hematocrite و بعدين بحطها في جهاز centrifuge أصلا
+
+465
+00:35:53,500 --> 00:35:57,870
+أيضا خاص في ..فحص الـ hematocrit بسميه hematocrit
+
+466
+00:35:57,870 --> 00:36:02,350
+centrifuge و بفصل بعدين بقيس على باستخدام meter
+
+467
+00:36:02,350 --> 00:36:08,410
+باستخدام مقياس مخصص لل hematocrit بشوف نسبة ال ..
+
+468
+00:36:08,410 --> 00:36:12,230
+ال .. ال RBC's كام في المية من اللي هو المية في
+
+469
+00:36:12,230 --> 00:36:17,160
+المية العينة كلهاتمام؟ هذا هو اللي راح يكون عنوان
+
+470
+00:36:17,160 --> 00:36:21,080
+محاضرتنا العملية اللي هي math of creative تمام؟
+
+471
+00:36:21,080 --> 00:36:26,040
+طيب إذا وصلنا لنهاية محاضرتنا اليوم أتمنى تكونوا
+
+472
+00:36:26,040 --> 00:36:29,540
+استفدتوا يكون الأشي بسيط بالنسبالي لكم أي سؤال ما
+
+473
+00:36:29,540 --> 00:36:32,580
+تترددوا تسألوني إما ب group ال whatsapp أو ان شاء
+
+474
+00:36:32,580 --> 00:36:38,000
+الله بال discussion الإسبوعي اللي احنا موعدنا كل
+
+475
+00:36:38,000 --> 00:36:42,600
+ثابت الساعة تماني على google meet و بتمنى لكم
+
+476
+00:36:42,600 --> 00:36:48,230
+التوفيقان شاء الله موعدنا يوم السبت الجاي على ال
+
+477
+00:36:48,230 --> 00:36:54,690
+google meet لحتى نتناقش بمحتوى المحلرة يعطيكم
+
+478
+00:36:54,690 --> 00:36:55,210
+العافية
+
diff --git a/PL9fwy3NUQKwY9jl8xw-bWkVZkWcGGyspd/KXPW3iBAdP0.srt b/PL9fwy3NUQKwY9jl8xw-bWkVZkWcGGyspd/KXPW3iBAdP0.srt
new file mode 100644
index 0000000000000000000000000000000000000000..7e1a2c55dac29e67f175be6c701b3ca021990ba5
--- /dev/null
+++ b/PL9fwy3NUQKwY9jl8xw-bWkVZkWcGGyspd/KXPW3iBAdP0.srt
@@ -0,0 +1,2803 @@
+1
+00:00:05,900 --> 00:00:09,780
+السلام عليكم ورحمة الله وبركاته، طالباتي العزيزات، إن
+
+2
+00:00:09,780 --> 00:00:13,380
+شاء الله، اليوم رح نكون مع بعض بمحاضرة عملية بعنوان
+
+3
+00:00:13,380 --> 00:00:19,080
+micro-hematocrit، طبعاً micro-hematocrit، ولكن قبل ما
+
+4
+00:00:19,080 --> 00:00:24,220
+نبدأ بمحاضرة micro-hematocrit، خلونا نسترجع مع بعض
+
+5
+00:00:24,220 --> 00:00:28,060
+إحنا إيش أخذنا بالمحاضرة النظرية السابقة؟ تحدثنا عن
+
+6
+00:00:28,060 --> 00:00:32,280
+جهاز الـ centrifuge، حكينا عن مكونات جهاز الـ
+
+7
+00:00:32,280 --> 00:00:36,140
+centrifuge، حكينا أيضاً على أنواع جهاز الـ centrifuge،
+
+8
+00:00:36,140 --> 00:00:40,200
+وعلى مبدأ عمله، وقوة التردد المركزي اللي من خلالها
+
+9
+00:00:40,200 --> 00:00:45,640
+بيتنفصل العينات، وبحيث أنه بترسب الأجزاء الثقيلة
+
+10
+00:00:45,640 --> 00:00:49,520
+أو المكونات الثقيلة في العينة في الـ bottom of the tube،
+
+11
+00:00:49,520 --> 00:00:53,650
+والأجزاء الخفيفة بتكون at the surface أو at the top،
+
+12
+00:00:53,650 --> 00:01:00,030
+تمام؟ وايضاً تحدثنا عن التعليمات الهامة للاستخدام،
+
+13
+00:01:00,030 --> 00:01:03,910
+instantaneous refusal، حكينا أنه لازم أنا أَتبع الـ
+
+14
+00:01:03,910 --> 00:01:07,290
+manufacturer recommendations، اللي هي توصيات الجهة
+
+15
+00:01:07,290 --> 00:01:12,430
+المصنعة، وإنه أنا أيضاً لازم آخذ بعين الاعتبار بعض
+
+16
+00:01:12,430 --> 00:01:17,410
+التعليمات، زي مثلاً أن أحط الجهاز على بنش ثابت، أسّكّف
+
+17
+00:01:17,410 --> 00:01:21,490
+الجهاز قبل ما أنا أشغله، ممنوع أوقف الجهاز إيقافاً
+
+18
+00:01:21,490 --> 00:01:24,470
+مفاجئاً، أو أحاول أن أنا أبطئ سرعته بإيدي أثناء
+
+19
+00:01:24,470 --> 00:01:29,470
+إيقافه، وغيرها من التعليمات اللي يجب مراعاتها ويجب
+
+20
+00:01:29,470 --> 00:01:34,130
+اتباعها أثناء العمل أو تشغيل جهاز الـ centrifuge.
+
+21
+00:01:34,680 --> 00:01:40,120
+كتوظيف لمعرفتنا حول جهاز الـ centrifuge، رح نأخذ
+
+22
+00:01:40,120 --> 00:01:44,220
+تطبيقاً عليه، وهو فحص الـ micro hematocrit، خلونا
+
+23
+00:01:44,220 --> 00:01:46,980
+بالأول نشوف إيش يعني كلمة micro hematocrit؟
+
+24
+00:01:46,980 --> 00:01:54,280
+hematocrit لحالها هي عبارة عن نسبة الـ RBCs بالنسبة
+
+25
+00:01:54,280 --> 00:01:58,380
+للـ whole blood، أو الـ volume اللي بتحتله الـ RBCs،
+
+26
+00:01:58,380 --> 00:02:02,080
+الحجم اللي بتحتله الـ RBCs بالنسبة للحجم الكل لعينة
+
+27
+00:02:02,080 --> 00:02:07,040
+الدم، تمام؟ النسبة المئوية للـ RBCs بالنسبة للـ
+
+28
+00:02:07,040 --> 00:02:09,810
+whole blood، أو عينة الـ whole blood، و
+
+29
+00:02:09,810 --> 00:02:14,650
+microhematocrit هو يعني إضافة كلمة micro  إلى hematocrit،
+
+30
+00:02:14,650 --> 00:02:20,210
+ما هي إلا تعبير عن أن أنا لما بشتغل الفحص هذا
+
+31
+00:02:20,210 --> 00:02:27,600
+بأخذ small amount of sample، micro sample، من خلال الـ
+
+32
+00:02:27,600 --> 00:02:32,560
+skin puncture، طبعاً من خلال الـ skin puncture بشتغل،
+
+33
+00:02:32,560 --> 00:02:35,140
+طبعاً إحنا حكينا micro sampling أو الـ skin puncture
+
+34
+00:02:35,140 --> 00:02:40,660
+نفس المعنى، فإذن الـ micro sampling إنه أنا بأخذ
+
+35
+00:02:40,660 --> 00:02:46,320
+عينة حجمها صغير في tube دقيق جداً، رفيع جداً، micro
+
+36
+00:02:46,320 --> 00:02:50,680
+tube، طبعاً عشان هيك صار اسمه micro hematocrit، طبعاً
+
+37
+00:02:50,680 --> 00:02:55,000
+إذن إضافة micro فقط تعبر عن حجم العينة وإنه
+
+38
+00:02:55,000 --> 00:02:58,340
+أنا بأخذ كمية قليلة من الـ blood لحتى أشتغل هذا
+
+39
+00:02:58,340 --> 00:03:02,320
+الفحص، micro hematocrit، له اسم آخر، أو الـ
+
+40
+00:03:02,320 --> 00:03:06,640
+hematocrit له اسم آخر، packed cell volume of whole
+
+41
+00:03:06,640 --> 00:03:10,020
+blood، إيش يعني packed cell volume؟ يعني أنا بدي
+
+42
+00:03:10,020 --> 00:03:15,720
+آخذ الحجم اللي بتحتله الـ RBCs، الخلايا، بعد ما
+
+43
+00:03:15,720 --> 00:03:18,900
+يصير لها packaging أو packing، بعد ما يصير إيش صار لها؟
+
+44
+00:03:18,900 --> 00:03:22,180
+packaging، يعني بعد ما صار لها sedimentation أو
+
+45
+00:03:22,180 --> 00:03:25,480
+spinning أو centrifugation أو separation، أنا يعني
+
+46
+00:03:25,480 --> 00:03:31,180
+جمعت الـ RBCs، لميتها على بعضها، يعني رسّبتها، أو يعني
+
+47
+00:03:31,180 --> 00:03:36,940
+حصرتها في volume معين، وبدي أشوف نسبة الـ volume
+
+48
+00:03:36,940 --> 00:03:40,960
+هذا، كم بتحتله نسبة الـ RBCs بالنسبة للـ whole blood،
+
+49
+00:03:40,960 --> 00:03:44,880
+بالنسبة لحجم العينة كلها. إذن، hematocrit is
+
+50
+00:03:44,880 --> 00:03:49,900
+defined as the volume occupied by erythrocytes in a
+
+51
+00:03:49,900 --> 00:03:54,180
+given volume of blood and is usually expressed as
+
+52
+00:03:54,180 --> 00:03:56,780
+a percentage of the volume of the whole blood
+
+53
+00:03:56,780 --> 00:04:01,240
+sample. The hematocrit may also be referred to as
+
+54
+00:04:01,240 --> 00:04:05,660
+packed cell volume. الهيماتوكريت يُعرّف على أنه
+
+55
+00:04:05,660 --> 00:04:10,440
+إيش؟ الـ volume occupied، الحجم اللي بتحتله مين؟ الـ
+
+56
+00:04:10,440 --> 00:04:15,780
+RBCs فقط، الـ RBCs، تمام؟ وطبعاً بالنسبة لإيش؟
+
+57
+00:04:15,780 --> 00:04:19,100
+بالنسبة لـ a given volume of blood، بالنسبة للحجم
+
+58
+00:04:19,100 --> 00:04:24,700
+اللي هو الـ total blood أو الـ whole blood، تمام؟ والـ
+
+59
+00:04:24,700 --> 00:04:28,560
+hematocrit بنسميه packed cell volume، وعرفنا إيش يعني
+
+60
+00:04:28,560 --> 00:04:32,780
+كلمة أو الترجمة الحرفية لـ packed cell volume، فباختصار
+
+61
+00:04:32,780 --> 00:04:37,060
+شديد، إحنا اليوم رح نشتغل، أو يعني رح نتعلم كيف نكتب و
+
+62
+00:04:37,060 --> 00:04:40,820
+بنعمل الـ micro hematocrit، من خلال أنه أنا أجيب
+
+63
+00:04:40,820 --> 00:04:45,000
+tube رفيعة جداً، دقيقة جداً، بسميها capillary tube،
+
+64
+00:04:45,000 --> 00:04:49,420
+طبعاً هذه الـ capillary tube بتكون مفتوحة الطرفين، و أنا بـ
+
+65
+00:04:49,420 --> 00:04:54,000
+أحدث من خلال skin puncture،
+
+66
+00:04:54,000 --> 00:04:57,380
+puncture في الـ skin، أو من خلال أن أنا أغمسها في
+
+67
+00:04:57,380 --> 00:05:01,780
+الـ blood، فالـ الدم راح يطلع في هذه الـ blood،
+
+68
+00:05:01,780 --> 00:05:05,200
+أو راح يملأ هذه الـ capillary، الـ blood راح يطلع
+
+69
+00:05:05,200 --> 00:05:08,940
+يملأ هذه الـ capillary بالخاصية الشعرية، عشان هيك
+
+70
+00:05:08,940 --> 00:05:12,300
+بسميها capillary tube، طبعاً بالـ capillary action
+
+71
+00:05:12,300 --> 00:05:16,440
+بيملأ الـ tube بالخاصية الشعرية، الـ capillary action،
+
+72
+00:05:16,440 --> 00:05:20,250
+عشان هيك الـ tube اسمها capillary tube، طبعاً طيب بعد
+
+73
+00:05:20,250 --> 00:05:23,990
+ما أنا أملأ الـ tube لحتى تقريباً ثلاثة أرباعها، رح
+
+74
+00:05:23,990 --> 00:05:28,950
+أسكر طرفاً من أطرافها باستخدام clay، إيش يعني clay؟
+
+75
+00:05:28,950 --> 00:05:32,290
+معجونة أو طينة، طينة اللي بيلعبوا فيها
+
+76
+00:05:32,290 --> 00:05:34,930
+الأطفال، هي اللي بتكون في ألعاب الأطفال، هي اللي
+
+77
+00:05:34,930 --> 00:05:40,020
+باستخدمها، طبعاً، طيب أصبح الـ tube صارت مغلقة الطرفين،
+
+78
+00:05:40,020 --> 00:05:43,980
+ولو أنا حطيتها في جهاز centrifuge خاص فيها، بسميه
+
+79
+00:05:43,980 --> 00:05:48,860
+micro hematocrit centrifuge، تمام؟ فراح يفصلها لي
+
+80
+00:05:48,860 --> 00:05:53,340
+بحيث أنه الأجزاء الثقيلة راح تكون at the bottom،
+
+81
+00:05:54,260 --> 00:05:57,440
+والأجزاء الخفيفة زي الـ plasma راح تكون at the top،
+
+82
+00:05:57,440 --> 00:06:04,000
+فأنا بحسب كم، أو يعني كم تحتل الـ RBCs بالنسبة
+
+83
+00:06:04,000 --> 00:06:07,760
+للـ whole blood، وبيطلع عندي قراءة الـ hematocrit، طب
+
+84
+00:06:07,760 --> 00:06:10,280
+أنا لما أحكي مثلاً الـ hematocrit ثلاثين، الـ
+
+85
+00:06:10,280 --> 00:06:15,910
+hematocrit أربعين، ستة وثلاثين، ستة وثلاثين، ٣٦
+
+86
+00:06:15,910 --> 00:06:21,770
+بالمئة، إذن هو بنعبر عنه as a percentage، تمام؟
+
+87
+00:06:21,770 --> 00:06:25,990
+إذن بنعبر عنه as a percentage، نسبة مئوية، كم
+
+88
+00:06:25,990 --> 00:06:29,510
+تحتل نسبة الـ RBCs بالنسبة للـ whole blood؟ أنا
+
+89
+00:06:29,510 --> 00:06:33,610
+بعتبر أن العينة كلها اللي أنا جمعتها مئة بالمئة،
+
+90
+00:06:33,610 --> 00:06:38,650
+كم الـ RBCs منها؟ فرح نشوف كيف أنا ممكن أقيسها،
+
+91
+00:06:38,650 --> 00:06:43,070
+تمام؟ إذن هذه هي فكرة الفحص، إذن الـ principle، مبدأ
+
+92
+00:06:43,070 --> 00:06:48,790
+عمل الفحص، أو فكرة الفحص، أو يعني الافتراض أو نظرية
+
+93
+00:06:48,790 --> 00:06:53,350
+الفحص، مبدأ عمل الفحص هو أن أنا الـ hematocrit is
+
+94
+00:06:53,350 --> 00:06:56,630
+usually determined by spinning a blood-filled
+
+95
+00:06:56,630 --> 00:07:02,180
+capillary tube in a centrifuge. بعمل determination،
+
+96
+00:07:02,180 --> 00:07:07,020
+قياس، معرفة للـ hematocrit من خلال أن أنا أعمل
+
+97
+00:07:07,020 --> 00:07:11,700
+عملية spinning، centrifugation، separation،
+
+98
+00:07:11,700 --> 00:07:15,020
+sedimentation، لاحظين المصطلحات دي كلها نفس المعنى،
+
+99
+00:07:15,020 --> 00:07:18,180
+للـ blood في الـ capillary، blood في الـ capillary،
+
+100
+00:07:18,180 --> 00:07:21,480
+capillary ممتلئة بالـ blood، من خلال الـ centrifuge،
+
+101
+00:07:21,480 --> 00:07:26,180
+هذه الـ capillary tube أنا عبّيتها لثلاثة أرباعها بالـ
+
+102
+00:07:26,180 --> 00:07:31,410
+blood، سكّرتها من الطرفين، أصبحت مغلقة، أحد الأطراف مغلق،
+
+103
+00:07:31,410 --> 00:07:36,330
+وهذا before centrifugation، after centrifugation، راح يصير
+
+104
+00:07:36,330 --> 00:07:40,310
+فيه packing للـ RBCs، الـ RBCs كلها راح
+
+105
+00:07:40,310 --> 00:07:44,890
+تتركّز في منطقة واحدة، اللي هي منطقة القاع، يليها الـ
+
+106
+00:07:44,890 --> 00:07:47,830
+buffy coat، ويليها الـ plasma، هذه الـ plasma هذه
+
+107
+00:07:47,830 --> 00:07:53,260
+كلها plasma، تمام؟ طبعاً فأنا بدي أشوف كم تحتل الـ
+
+108
+00:07:53,260 --> 00:07:57,600
+RBCs بالنسبة للـ whole blood، لو افترضت أن هذه كلها
+
+109
+00:07:57,600 --> 00:08:02,600
+مئة بالمئة، كم الـ RBCs من المئة بالمئة هذول؟ طبعاً طيب، العينة
+
+110
+00:08:02,600 --> 00:08:07,280
+تبعيتي بأخذها إما venous blood، يعني إما دم من
+
+111
+00:08:07,280 --> 00:08:12,350
+الوريد، بجمعه، أو من الـ skin puncture، طبعاً إذا
+
+112
+00:08:12,350 --> 00:08:16,790
+أنا سحبت دم من الوريد وحطيته في EDTA tube، في الـ
+
+113
+00:08:16,790 --> 00:08:21,070
+tube اللي لونها lavender، الـ cap تبعها لونه mauve،
+
+114
+00:08:21,070 --> 00:08:24,950
+lavender، طبعاً هذا بيكون فيه EDTA anticoagulant،
+
+115
+00:08:24,950 --> 00:08:31,720
+طبعاً، فإذا أنا حطيته فيه، بس ممكن بعدها أعبي أو أملأ
+
+116
+00:08:31,720 --> 00:08:36,060
+capillary من خلاله، أو ممكن مباشرة زي هنا في الصورة
+
+117
+00:08:36,060 --> 00:08:40,100
+من الـ skin puncture، أملأ الـ capillary tube، بس في
+
+118
+00:08:40,100 --> 00:08:44,040
+عندي شغلة، الـ capillary tube، لو أنا هنا استعرضت
+
+119
+00:08:44,040 --> 00:08:48,900
+معاكم كيف شكل الـ capillary tube وكيف أنواعها، هذه
+
+120
+00:08:48,900 --> 00:08:52,640
+زي ما أنتم شايفين هنا في عندي capillary tube،
+
+121
+00:08:55,110 --> 00:08:58,450
+هَيْها، هذه الـ capillary tube، هيك بتكون، طبعاً بتكون
+
+122
+00:08:58,450 --> 00:09:03,510
+عندي علبة يعني فيها أكثر، يعني تقريباً مئة tube، الـ
+
+123
+00:09:03,510 --> 00:09:07,370
+capillary tube، هذه يا بنات، طبعاً إلها color code،
+
+124
+00:09:07,370 --> 00:09:12,670
+نفس فكرة الأنابيب، وكل لون يعني إنه هي من الداخل
+
+125
+00:09:12,670 --> 00:09:17,340
+ممسوحة بنوع معين من الـ anticoagulant، تمام؟ هذه اللي
+
+126
+00:09:17,340 --> 00:09:21,580
+لونها أحمر، ممسوحة من الداخل بـ heparin، فهذه تُستخدم
+
+127
+00:09:21,580 --> 00:09:26,620
+مباشرة لـ blood اللي من skin،
+
+128
+00:09:26,620 --> 00:09:31,000
+puncture، لكن مثلاً في هذه عندي نوع آخر من الـ
+
+129
+00:09:31,000 --> 00:09:38,090
+capillary، زي اللي لونها blue طبعاً، blue، فهذا النوع
+
+130
+00:09:38,090 --> 00:09:41,730
+من الـ capillary فاضي، ما فيه شيء، مش ممسوح بالداخل بـ
+
+131
+00:09:41,730 --> 00:09:44,790
+anticoagulant، بسميها bland، إحنا اتفقنا، إذا ما فيه
+
+132
+00:09:44,790 --> 00:09:48,270
+فيه شيء بالـ tube، بسميها bland، نفس الشيء، capillary
+
+133
+00:09:48,270 --> 00:09:52,470
+لأنها مش، من الداخل ما فيها anticoagulant، فهي
+
+134
+00:09:52,470 --> 00:09:57,780
+bland capillary، طبعاً هذه بجمع فيها أو بأملأها من الدم
+
+135
+00:09:57,780 --> 00:10:01,780
+اللي من الـ EDTA tube، يعني لو أنا جمعت blood من الوريد،
+
+136
+00:10:01,780 --> 00:10:05,300
+وحطيته في EDTA tube، بعدين بدي أملأ capillary وأشتغل
+
+137
+00:10:05,300 --> 00:10:09,840
+micro hematocrit، بستخدم اللي bland، لكن لو بدي
+
+138
+00:10:09,840 --> 00:10:13,680
+أعمل skin puncture ومباشرة أملأ capillary من الـ
+
+139
+00:10:13,680 --> 00:10:16,660
+skin puncture، بستخدم الـ heparinized، دي اللي لونها
+
+140
+00:10:16,660 --> 00:10:23,540
+أحمر، طبعاً ما ينفع، ما ينفع أنا أجمع عينة blood في
+
+141
+00:10:23,540 --> 00:10:28,080
+EDTA tube وأروح أستخدم الـ heparinized capillary،
+
+142
+00:10:28,080 --> 00:10:33,800
+معاها، يعني ما ينفع، ما ينفع أنا، ما ينفع أمرر
+
+143
+00:10:33,800 --> 00:10:37,580
+الـ blood على نوعين anticoagulant، بالأول EDTA في
+
+144
+00:10:37,580 --> 00:10:42,460
+tube، بعدين الـ heparin في الـ capillary، ممنوع، ليش؟ لأن
+
+145
+00:10:42,460 --> 00:10:48,060
+هذه الـ anticoagulant عبارة عن salts، أملاح، طبعاً،
+
+146
+00:10:48,060 --> 00:10:54,000
+طالما إنها أملاح، وجدت في العينة، راح تأثر على
+
+147
+00:10:54,000 --> 00:10:57,980
+الضغط الأسموزي، على الـ osmolality للعينة، والضغط
+
+148
+00:10:57,980 --> 00:11:03,080
+الأسموزي للخلايا، فبما إنها أثّرت على الضغط الأسموزي
+
+149
+00:11:03,080 --> 00:11:07,640
+للخلايا، يعني اختلف شكل الخلايا، طبعاً اختلف شكل
+
+150
+00:11:07,640 --> 00:11:13,910
+الخلايا، وطالما اختلف شكلها، إذن راح تتأثر قراءة الـ
+
+151
+00:11:13,910 --> 00:11:18,700
+hematocrit، وراح نشرح هذا لاحقاً خلال المحاضرات
+
+152
+00:11:18,700 --> 00:11:23,320
+الجاية، طبعاً الـ specimen لازم أنا
+
+153
+00:11:23,320 --> 00:11:26,620
+العينة تبعيتي، سواء كان يعني specimen هي نفسها
+
+154
+00:11:26,620 --> 00:11:30,900
+معناها sample، نفس الشيء، عينة، طبعاً العينة تبعيتي
+
+155
+00:11:30,900 --> 00:11:34,200
+لازم أنا أفصلها باستخدام الـ centrifuge، should be
+
+156
+00:11:34,200 --> 00:11:37,820
+centrifuged within six hours of collection، يعني
+
+157
+00:11:37,820 --> 00:11:43,940
+خلال ست ساعات من جمع العينة، لازم أنا أكون عملت
+
+158
+00:11:43,940 --> 00:11:50,140
+centrifugation، لازم أكون عملت centrifugation، ليش؟
+
+159
+00:11:50,140 --> 00:11:55,260
+لأنّه إذا أنا بدي أتأخر أكثر من هيك، ممكن تتأثر، يعني
+
+160
+00:11:55,260 --> 00:12:00,540
+تتغير مكونات العينة، في خلايا ممكن تتكسر، تتحلّل،
+
+161
+00:12:00,540 --> 00:12:03,560
+يصير في عندي فاقد في الخلايا، وأنا أصلاً قراءتي هي
+
+162
+00:12:03,560 --> 00:12:06,560
+عبارة عن نسبة خلايا، حجم اللي بتحتله الخلايا،
+
+163
+00:12:06,560 --> 00:12:10,160
+بالنسبة للـ whole blood، فإذا صار في عندي فاقد، إذن
+
+164
+00:12:10,160 --> 00:12:14,680
+هيكون في عندي يعني فاقد أيضاً في القراءة، في عندي
+
+165
+00:12:14,680 --> 00:12:21,060
+نسبة قراءة ناقصة، يعني decreased، طبعاً، في قراءة
+
+166
+00:12:21,060 --> 00:12:25,920
+الـ hematocrit. Hemolyzed samples can't be used for
+
+167
+00:12:25,920 --> 00:12:31,300
+testing، عينة تمت، يعني صار فيها تكسر في الخلايا،
+
+168
+00:12:31,300 --> 00:12:36,260
+أيضاً مش proper، مش جيدة لحتى أنا أشتغل فيها
+
+169
+00:12:36,260 --> 00:12:41,500
+hematocrit، why؟ لأن الـ hemolyzed sample بيصير فيه
+
+170
+00:12:41,500 --> 00:12:45,240
+فقد عندي في الخلايا، اتكسرت خلايا الـ RBCs، طب أنا
+
+171
+00:12:45,240 --> 00:12:50,140
+أصلاً عم بحسب حجم الـ RBCs، حجم الـ erythrocytes،
+
+172
+00:12:50,1
+
+223
+00:16:36,390 --> 00:16:42,010
+أنه تفهموها لأنها مهمة يا بنات. طيب بعد ما أنا أملأ
+
+224
+00:16:42,010 --> 00:16:45,370
+الـ tube بتلات ... بتلات أرباعها. طبعا حكينا إن الـ
+
+225
+00:16:45,370 --> 00:16:49,290
+blood بيملأها بالخاصية الشعرية capillary action
+
+226
+00:16:49,290 --> 00:16:53,450
+seal the end of the tube with the non-coloring
+
+227
+00:16:53,450 --> 00:17:00,510
+ring with the non-absorbent clay. هلأ يعني إنه من
+
+228
+00:17:00,510 --> 00:17:07,750
+الجهة المش ملونة مثلا. سكري الـ capillary بالـ clay
+
+229
+00:17:07,750 --> 00:17:16,040
+يعني فوتيها ... يعني هيك ... فوتيها في داخل الـ container
+
+230
+00:17:16,040 --> 00:17:20,560
+أو يعني bottle أو cup موجود فيه الـ clay فهتلاحظي
+
+231
+00:17:20,560 --> 00:17:24,820
+أن الـ clay هيدخل جوا الـ ... الـ capillary ويسكر
+
+232
+00:17:24,820 --> 00:17:29,940
+الفتحة أحد الطرفين. balance the tube in the
+
+233
+00:17:29,940 --> 00:17:32,600
+centrifuge with the clay. طبعا أنا بستخدم two
+
+234
+00:17:32,600 --> 00:17:36,520
+capillary للعينة الواحدة. why؟ أو ليش؟ عشان أنا
+
+235
+00:17:36,520 --> 00:17:40,550
+أعمل الـ ... الـ ... الـ tube بدها balance تمام؟ ثاني
+
+236
+00:17:40,550 --> 00:17:44,530
+شيء لإنه ب ... بجيب القراءة على الـ two capillaries
+
+237
+00:17:44,530 --> 00:17:49,670
+tube وبعدين ب ... بجيب الـ average لإلهم. وطالما
+
+238
+00:17:49,670 --> 00:17:52,170
+أنا جبت الـ average لقراقتين معناها أنا more
+
+239
+00:17:52,170 --> 00:17:57,280
+accurate and precise. بحط الأنابيب في الـ centrifuge
+
+240
+00:17:57,280 --> 00:18:03,100
+مقابل بعضها البعض. وبعد هيك بشغل جهاز الـ
+
+241
+00:18:03,100 --> 00:18:07,040
+centrifuge لمدة خمس دقائق على سرعة ما بين العشر
+
+242
+00:18:07,040 --> 00:18:12,060
+ألف إلى خمسة عشر ألف round per minute. use the brake
+
+243
+00:18:12,060 --> 00:18:14,980
+to stop the centrifuge. ممنوع أنا أستخدم الـ brake
+
+244
+00:18:14,980 --> 00:18:19,060
+لحتى أوقف الـ centrifuge لإنه إذا حكينا إحنا قبل
+
+245
+00:18:19,060 --> 00:18:23,080
+هيك إنه أنا إذا عملت إيقاف مفاجئ للـ centrifuge
+
+246
+00:18:23,080 --> 00:18:27,920
+معناها الـ sediment اللي اترسب راح يندفع باتجاه
+
+247
+00:18:27,920 --> 00:18:30,780
+الأعلى. فبالتالي راح يصير في mixing للعينة أو
+
+248
+00:18:30,780 --> 00:18:34,520
+انفصال للـ sediment وهذا راح يعطيني error، خطأ في
+
+249
+00:18:34,520 --> 00:18:40,050
+القراءة. طبعا بعد ما يتوقف الجهاز، لأ يعني بعد ما
+
+250
+00:18:40,050 --> 00:18:43,670
+ينتهي، بيقول خمس دقائق بيرن جرس يعني عشان تنبيه
+
+251
+00:18:43,670 --> 00:18:49,870
+أن انتهت المدة وبيبدأ الجهاز يبطئ سرعته بنفسه
+
+252
+00:18:49,870 --> 00:18:56,550
+طبعا إلى أن يقف، يعني to stop. بعد ما الجهاز يعني تـ
+
+253
+00:18:56,550 --> 00:19:03,070
+stop، يقف ب ... أنا بفتح الـ ... الـ cover تبعه وباخد
+
+254
+00:19:03,070 --> 00:19:07,770
+القنابيب. وباستخدام تدريج خاص ب ... بعمل
+
+255
+00:19:07,770 --> 00:19:10,430
+determination للـ hematocrit، بعرف الـ hematocrit
+
+256
+00:19:13,130 --> 00:19:16,330
+كيف هذا ... فكرة الـ ... الـ reader تبعي؟ زي ما أنتم
+
+257
+00:19:16,330 --> 00:19:20,330
+شايفين، هو عبارة عن تدريج بيبدأ بالصفر وبينتهي
+
+258
+00:19:20,330 --> 00:19:26,090
+بالمئة، طبعا؟ هذا التدريج بتشوفوه زي عم بيتسع كل
+
+259
+00:19:26,090 --> 00:19:30,330
+ما اتجهنا بهذا الاتجاه، طبعا؟ ضيق هنا وبيتسع هنا
+
+260
+00:19:30,330 --> 00:19:34,830
+ونفس الفكرة أن الـ ... الـ ... بدايته صفر ونهايته
+
+261
+00:19:34,830 --> 00:19:39,080
+مئة. طب ليش ... ليش عم بالتساع؟ لحتى يناسب جميع
+
+262
+00:19:39,080 --> 00:19:42,280
+الأطوال وجميع محتويات العينة. يعني أنا لو ... لو
+
+263
+00:19:42,280 --> 00:19:46,300
+عينة طويلة أو tube طويلة أو عينة قصيرة، tube
+
+264
+00:19:46,300 --> 00:19:49,440
+ما ملقتها كتير، tube ما ... ما ... ما عبيتها كتير
+
+265
+00:19:49,440 --> 00:19:53,640
+فبالتالي أن في النهاية يكون التدريج مناسب لإلها،
+
+266
+00:19:53,640 --> 00:19:56,040
+لا tube طويلة و الله بروح على الجهة الطويلة من
+
+267
+00:19:56,040 --> 00:20:01,540
+التدريج، tube قصيرة أو يعني tube محتواها قليل،
+
+268
+00:20:01,540 --> 00:20:06,260
+بروح على الجهة القصيرة من التدريج. بروح بطابق يعني
+
+269
+00:20:06,260 --> 00:20:09,140
+زي ما أنتم شايفين، بداية الـ blood لازم تكون عند خط
+
+270
+00:20:09,140 --> 00:20:13,720
+الصفر. ونهاية الـ plasma لازم تكون متطابقة لخط
+
+271
+00:20:13,720 --> 00:20:18,340
+المئة. بعدين بشوف الـ RBCs وبقرأ من تحت الـ buffer
+
+272
+00:20:18,340 --> 00:20:23,880
+code بشوف هي مقابل لأي قراءة وبعرف كم القراءة الـ
+
+273
+00:20:23,880 --> 00:20:27,820
+RBCs، يعني كم نسبة الـ RBCs بالنسبة للـ whole blood
+
+274
+00:20:27,820 --> 00:20:32,920
+تمام؟ هي فكرة استخدام التدريج. عندنا أنواع و ...
+
+275
+00:20:32,920 --> 00:20:35,860
+يعني أشكال مختلفة من التدريج. راح إن شاء الله بس
+
+276
+00:20:35,860 --> 00:20:38,700
+نرجع المختبرات راح أعلمكم عليها وكيف تستخدموها
+
+277
+00:20:38,700 --> 00:20:44,640
+تمام؟ بحكي لي وأنت بتقرأ إيه؟ don't include the
+
+278
+00:20:44,640 --> 00:20:48,020
+buffy coat, leukocyte and the platelet. why؟ احنا
+
+279
+00:20:48,020 --> 00:20:51,260
+بنقرأ micro hematocrit. الـ volume occupied by
+
+280
+00:20:51,260 --> 00:20:57,520
+erythrocyte by erythrocyte. طبعا؟ الحجم اللي بتحتله
+
+281
+00:20:57,520 --> 00:21:03,330
+الـ RBCs فقط. لذلك أنا ما بدخل ... ما ... الـ ...
+
+282
+00:21:03,330 --> 00:21:06,610
+الـ ... يعني ما بحسب الـ ... الـ buffy coat في القراءة
+
+283
+00:21:06,610 --> 00:21:10,210
+don't include the buffy coat in the reading. تمام؟
+
+284
+00:21:10,210 --> 00:21:13,970
+ممنوع تدخلي أو يعني تحسبي الـ buffy coat داخل
+
+285
+00:21:13,970 --> 00:21:18,170
+القراءة. طب أنا بقرأ من تحت الـ buffy coat تمام؟
+
+286
+00:21:18,170 --> 00:21:21,490
+اللازم الـ tubes ... الـ tubes تبعتي، الـ two tubes
+
+287
+00:21:21,490 --> 00:21:26,160
+يكون على الأقل ... يعني أو يعني على ... على أكثر الفرق
+
+288
+00:21:26,160 --> 00:21:29,680
+ما بينهم اثنين بالمئة، إيش يعني؟ يعني ممكن يكون
+
+289
+00:21:29,680 --> 00:21:33,740
+الفرق ما بينهم نصف بالمئة، واحد بالمئة، اثنين
+
+290
+00:21:33,740 --> 00:21:37,180
+بالمئة، plus minus طبعا يا بنات، أكثر من هيك
+
+291
+00:21:37,180 --> 00:21:40,320
+ممنوع، يعني plus minus ثلاثة لأ، plus minus اثنين
+
+292
+00:21:40,320 --> 00:21:44,100
+ونصف لأ، معناها في variation كتير في النتائج هيك،
+
+293
+00:21:44,100 --> 00:21:46,140
+أنا ممنوع ... مش لازم يكون في عندي variation، لازم
+
+294
+00:21:46,140 --> 00:21:50,830
+يكون في تقارب في النتائج. تمام، إيش يعني الكلام هذا؟
+
+295
+00:21:50,830 --> 00:21:54,510
+لنفرض إنه في عندي tube اللي hematocrite فيها طلع
+
+296
+00:21:54,510 --> 00:22:00,910
+36%، تمام؟ معناها الـ tube الثانية كم لازم يطلع؟
+
+297
+00:22:00,910 --> 00:22:06,330
+ممكن يطلع 36 زيها بالظبط، ممكن 36.5 أو 35.5 يعني
+
+298
+00:22:06,330 --> 00:22:11,250
+plus minus نصف، تمام؟ ممكن يكون خمسة وثلاثين أو
+
+299
+00:22:11,250 --> 00:22:14,890
+سبعة وثلاثين plus minus واحد هيك تمام؟ ممكن
+
+300
+00:22:14,890 --> 00:22:19,830
+يكونوا أربعة وثلاثين أو ثمانية وثلاثين هيك plus
+
+301
+00:22:19,830 --> 00:22:25,170
+minus اثنين. بس نهائيا ... نهائيا ما بقبل نتيجة إذا
+
+302
+00:22:25,170 --> 00:22:29,130
+كانت واحدة فيهم قيمتها ستة وثلاثين مثلا والقيمة
+
+303
+00:22:29,130 --> 00:22:31,610
+الثانية ثلاثة وثلاثين، يعني في فرق ما بينهم ثلاثة
+
+304
+00:22:31,610 --> 00:22:34,910
+بالمئة. أو إذا كانت ستة وثلاثين والثانية تسعة و
+
+305
+00:22:34,910 --> 00:22:38,030
+ثلاثين، في فرق ما بينهم ثلاثة بالمئة، غلط. لازم
+
+306
+00:22:38,030 --> 00:22:40,910
+within اثنين بالمئة يكون الفرق ما بينهم علشان
+
+307
+00:22:40,910 --> 00:22:45,790
+أحكي والله إنه في تقارب في نتيجة تبعتي. تمام؟ باخد
+
+308
+00:22:45,790 --> 00:22:49,530
+القيمتين بقرأ الـ ... الـ ... الـ two tubes وبجيب الـ
+
+309
+00:22:49,530 --> 00:22:52,870
+average تبعهم علشان أكون more accurate and precise
+
+310
+00:22:54,880 --> 00:22:59,440
+طيب خلينا بس نشوف كيف شكل الـ centrifuge اللي احنا
+
+311
+00:22:59,440 --> 00:23:02,160
+... احنا حكينا أن في عندي micro hematocrit
+
+312
+00:23:02,160 --> 00:23:05,840
+centrifuge. هاي شكل الـ centrifuge. طبعا هذا typical
+
+313
+00:23:05,840 --> 00:23:09,380
+موجود عندنا بالمختبرات زيه، typically، يعني أخوه،
+
+314
+00:23:09,380 --> 00:23:13,710
+نفس الألوان، نفس الشكل. هذا الـ cover الخارجي وفي
+
+315
+00:23:13,710 --> 00:23:18,710
+cover داخلي. تمام، وهذا تدريج timer هو already set
+
+316
+00:23:18,710 --> 00:23:22,330
+على سرعة معينة. يعني أنا ما بحدد سرعته. هذا الجرس
+
+317
+00:23:22,330 --> 00:23:26,590
+الإنذار، أنه يعني توقف الجهاز، وهذا الـ break. تمام.
+
+318
+00:23:26,590 --> 00:23:30,730
+لو جيت أنا فتحت الـ cover الخارجي والداخلي، كيف رح
+
+319
+00:23:30,730 --> 00:23:35,860
+يكون شكل الـ ... الـ ... الـ hematocrit؟ زي ما أنتم
+
+320
+00:23:35,860 --> 00:23:41,320
+شايفين، بهذا الشكل بيكون شكل الـ ... الـ ... الـ ... الـ
+
+321
+00:23:41,320 --> 00:23:45,240
+micro hematocrit centrifuge من الداخل. الـ ... هذه الـ
+
+322
+00:23:45,240 --> 00:23:49,680
+head وهي فيها buckets، فيها فرزات. تمام؟ هذا الفرز
+
+323
+00:23:49,680 --> 00:23:52,980
+مخصص للـ capillary. هذا عشان هيك أنا بسميه
+
+324
+00:23:52,980 --> 00:23:56,520
+centrifuge خاص بالـ hematocrit، التيوب. لإنه هو
+
+325
+00:23:56,520 --> 00:23:59,940
+ما بيستوعب تيوبات كبيرة، هو بيستوعب فقط الـ ... الـ ...
+
+326
+00:23:59,940 --> 00:24:06,200
+الـ micro hematocrit. تمام؟ طيب، هذا بس الـ ... الـ ...
+
+327
+00:24:06,200 --> 00:24:10,280
+الشكل الـ micro centrifuge أو الـ micro hematocrit
+
+328
+00:24:10,280 --> 00:24:13,900
+الـ centrifuge من الداخل. هاي كمان صورة أخرى لإله.
+
+329
+00:24:13,900 --> 00:24:18,580
+شايفين؟ هاد ... هاد الـ central shaft، هادي الـ head
+
+330
+00:24:19,040 --> 00:24:24,000
+وفيها buckets طبعا؟ أنتم شوفتوا الـ buckets في الـ
+
+331
+00:24:24,000 --> 00:24:28,940
+centrifuges الثانية عبارة عن مكان زي وعاء بيشبه
+
+332
+00:24:28,940 --> 00:24:35,820
+شكل الـ tube طبعا؟ هذا أيضا فرز أو زي مكان مخصص
+
+333
+00:24:35,820 --> 00:24:39,780
+بيشبه شكل الـ capillary، بيشبه شكل الـ capillary tube
+
+334
+00:24:41,240 --> 00:24:46,060
+لذلك هو مخصص لـ micro-hematocrit. هذا الجهاز للـ
+
+335
+00:24:46,060 --> 00:24:48,600
+centrifuge هذه. شايفين هي الغطاية الداخلية وهي
+
+336
+00:24:48,600 --> 00:24:52,140
+الغطاية الخارجية. طبعا يا بنات لما بحط لي tubes
+
+337
+00:24:52,140 --> 00:24:57,760
+لازم أراعي الـ balance، كل tube مقابل الثانية، كل
+
+338
+00:24:57,760 --> 00:25:02,940
+tube مقابل الثانية. تمام؟ طيب وفي شغلة لازم
+
+339
+00:25:02,940 --> 00:25:08,160
+أنتبه لها، أنه لما أسكر الـ tube بالـ clay لازم
+
+340
+00:25:08,160 --> 00:25:12,840
+المنطقة المغلقة وين تكون؟ تكون على الحواف. تمام؟
+
+341
+00:25:12,840 --> 00:25:16,580
+تكون هنا المنطقة المغلقة أو هذه المنطقة المفتوحة
+
+342
+00:25:16,910 --> 00:25:21,910
+ليش بقى أننتبه لهاي الشغلة؟ لإنه هذا جهاز طارد
+
+343
+00:25:21,910 --> 00:25:24,830
+مركزي، الأجزاء ... الأجزاء الثقيلة وين راح تروح؟
+
+344
+00:25:24,830 --> 00:25:27,890
+راح تروح بعيد عن الـ central shaft، بعيد عن
+
+345
+00:25:27,890 --> 00:25:32,210
+المركز، وين راح تتطرد؟ بهذا الاتجاه، يعني
+
+346
+00:25:32,210 --> 00:25:36,380
+هنا الـ RBCs راح تتجمع. تخيلي لو المكان هذا مفتوح مش
+
+347
+00:25:36,380 --> 00:25:40,080
+مسكر بالـ clay، إيش يعني؟ يعني راح يصير ... يصير
+
+348
+00:25:40,080 --> 00:25:42,960
+طردها خالص، يعني إحنا طردناها و زيادة وكت ...
+
+349
+00:25:42,960 --> 00:25:49,160
+عملنا وكترنا، فبيصير إيش؟ طردنا الـ ... الـ ... الـ ...
+
+350
+00:25:49,160 --> 00:25:54,040
+الـ RBCs خارج التيوب وليس at the bottom of التيوب
+
+351
+00:25:54,540 --> 00:25:58,420
+طبعا. فعشان هيك لازم المنطقة المغلقة تكون في هذا
+
+352
+00:25:58,420 --> 00:26:02,920
+الاتجاه. عشان لما تيجي الـ RBCs يتم طردها مركزيا أو
+
+353
+00:26:02,920 --> 00:26:06,120
+يعني تترسب أو يصير لها sedimentation separation في
+
+354
+00:26:06,120 --> 00:26:09,620
+at the bottom of the tube، يكون فيها عائق على الأقل
+
+355
+00:26:09,620 --> 00:26:15,380
+يمنعها من أن هي يصير لها loss، فقدان. طبعا تخيلي
+
+356
+00:26:15,380 --> 00:26:19,150
+لو أنا عكستها، في ... بيصير في طارد مركزي، إيش يعني؟
+
+357
+00:26:19,150 --> 00:26:22,270
+أن أنا ماسكة التيوب وكابتها، هاي معناها، وكأنك
+
+358
+00:26:22,270 --> 00:26:27,010
+يا أبو زيد ما غزيت، تمام؟ أنا بكون كابسة إيدي ...
+
+359
+00:26:27,010 --> 00:26:34,860
+أنا ببي بإيدي. طيب الـ ... الـ ... الـ cover الداخلي
+
+360
+00:26:34,860 --> 00:26:38,740
+هذا بيغلق ... بيتم إغلاقه من خلال ترس يا بنات، اسمه
+
+361
+00:26:38,740 --> 00:26:44,440
+يعني بيحبك على الـ capillary tube. بعدين بسكر الـ
+
+362
+00:26:44,440 --> 00:26:48,160
+cover الخارجي. عشان هيك لازم أتأكد أن كل tube
+
+363
+00:26:48,160 --> 00:26:51,280
+موجودة داخل الفرز المخصص لإلها، مش طالعة على بره أو
+
+364
+00:26:51,280 --> 00:26:55,520
+بارزة. لإنه إذا كانت مش موجودة بالـ ... بالفرز يعني
+
+365
+00:26:55,520 --> 00:27:00,310
+تماماً، موجودة داخل الفرز المخصص لإلها. هاي الـ ... الـ
+
+366
+00:27:00,310 --> 00:27:05,050
+... الغطاية لما أجي أسكرها وتحبك راح تطحن الـ tube
+
+367
+00:27:05,050 --> 00:27:09,530
+الـ capillary راح تكسرها، تفتفتها، طبعا؟ وهيك أنا
+
+368
+00:27:09,530 --> 00:27:15,630
+بكون فقدت العينة تبعتي. فإحذروا أن تفقدوا العينة
+
+369
+00:27:15,630 --> 00:27:20,430
+تبعتكم. اتأكدي أن الـ ... الـ tube جالسة بالمكان
+
+370
+00:27:20,430 --> 00:27:25,350
+المخصص لإلها، بالفرز المخصص لإلها. ومنطقة المغلقة
+
+371
+00:27:25,350 --> 00:27:29,990
+بالـ clay هي اللي موجودة outside، بعيدة عن المركز، و
+
+372
+00:27:29,990 --> 00:27:34,590
+المنطقة المفتوحة هي اللي أقرب ما يكون لمركزها. تمام.
+
+373
+00:27:36,940 --> 00:27:41,140
+طيب بعد ما أنا خلصت وحكينا بـ ... بـ ... بحطها على
+
+374
+00:27:41,140 --> 00:27:46,400
+التدريج الخاص فيها وبحط بداية الـ blood عند خط الـ
+
+375
+00:27:46,400 --> 00:27:50,960
+zero ونهاية الـ plasma عند خط المئة. بقرا الـ ... الـ
+
+376
+00:27:50,960 --> 00:27:56,860
+... نسبة الـ RBCs بالنسبة يعني للـ whole blood ومن
+
+377
+00:27:56,860 --> 00:27:59,680
+تحت الـ buffy coat بقرا. يعني أنا بس بدي نسبة الـ ...
+
+378
+00:27:59,680 --> 00:28:04,430
+الـ erythrocyte فقط لا غير. طيب يا بنات، هذه الـ
+
+379
+00:28:04,430 --> 00:28:08,550
+reference ranges، يعني الـ normal range. حكينا إنه الـ
+
+380
+00:28:08,550 --> 00:28:14,070
+normal value أنا بعبّر عنها بـ range وليس قيمة
+
+381
+00:28:14,070 --> 00:28:18,890
+واحدة. لـ newborn، إيش قيمة الهيماتوكريت الطبيعية عندهم؟
+
+382
+00:28:18,890 --> 00:28:26,530
+من 53% لـ 65%. طب ليش أنا بحكي 53% لـ 65%؟ هي عالية
+
+383
+00:28:26,530 --> 00:28:29,970
+أنتم لاحظوا معي، أنا مقارنة بالقيم الثانية هي أعلى شيء
+
+384
+00:28:30,550 --> 00:28:34,990
+حكينا لإنه الـ newborn، الأطفال حديثي الولادة
+
+385
+00:28:34,990 --> 00:28:39,030
+بيولدوا عندهم نسبة الـ hemoglobin عالية كتير والـ
+
+386
+00:28:39,030 --> 00:28:42,310
+RBCs قيمتها بتكون عالية كتير. يعني عندهم خلايا
+
+387
+00:28:42,310 --> 00:28:46,750
+عالية، عندهم خلايا كتيرة. ليش؟ لإنه أول حاجة هم في
+
+388
+00:28:46,750 --> 00:28:50,810
+حالة growth burst، يعني انفجار في النمو عندهم، يعني
+
+389
+00:28:50,810 --> 00:28:56,030
+هم في حالة نمو هائل. تمام؟ ومحتاجين oxygen
+
+390
+00:28:56,030 --> 00:29:03,850
+delivery، يعني محتاجين RBCs كتيرة. وأيضا عندهم مشكلة
+
+391
+00:29:03,850 --> 0
+
+445
+00:33:16,360 --> 00:33:21,940
+عندكم الـ polysaccharide و زي كلمة poly عديد، طبعا؟
+
+446
+00:33:21,940 --> 00:33:25,480
+طيب، polysaccharide، polypeptide، poly .. كذا، أي
+
+447
+00:33:25,480 --> 00:33:31,340
+poly فهو عديد معناها كلمة .. كلمة poly  جاية
+
+448
+00:33:31,340 --> 00:33:36,510
+من كلمة خلايا، تمام؟ site جاي من كلمة خلايا .. site
+
+449
+00:33:36,510 --> 00:33:41,310
+.. cytology علم الخلايا .. site خلايا .. emia كأي
+
+450
+00:33:41,310 --> 00:33:46,790
+كلمة بتنتهي بـ emia يعني في الدم، تمام؟ يعني زي
+
+451
+00:33:46,790 --> 00:33:50,450
+.. خلايا عديدة في الدم، يعني زيادة عدد خلايا الدم
+
+452
+00:33:50,450 --> 00:33:55,660
+الـ RBCs، تمام؟ هاي معناها كلمة polycythemia طيب يا
+
+453
+00:33:55,660 --> 00:33:59,780
+بنات إيش يعني polycythemia؟ في بعض الحالات طبعا هي
+
+454
+00:33:59,780 --> 00:34:06,180
+مجموعة من الأمراض، مش مرض واحد، وبينتج عنه أن الجسم
+
+455
+00:34:06,180 --> 00:34:11,580
+يصنع خلايا RBCs زيادة عن الطبيعي، يعني مثلا لو الشخص
+
+456
+00:34:11,580 --> 00:34:14,620
+.. ممكن .. ممكن يكون الشخص نتيجة الظروف اللي بيعيش
+
+457
+00:34:14,620 --> 00:34:18,880
+فيها ممكن يصير عنده polycythemia، ممكن نتيجة organ
+
+458
+00:34:18,880 --> 00:34:23,280
+dysfunction، يعني مشكلة عنده في أحد أعضاء جسمه فأدت
+
+459
+00:34:23,280 --> 00:34:26,460
+إلى أن الجسم راح يصنع polycythemia .. يعني راح
+
+460
+00:34:26,460 --> 00:34:30,520
+يصنع RBCs بالزيادة فيكون عنده polycythemia أو حالة
+
+461
+00:34:30,520 --> 00:34:34,360
+الـ tumor ورم يعني الخلايا بتتكثر بشكل مش طبيعي
+
+462
+00:34:35,110 --> 00:34:38,850
+تمام؟ طيب نبدأ نحكي عنهم واحدة واحدة بالدور الـ ..
+
+463
+00:34:38,850 --> 00:34:42,450
+الـ .. الظروف اللي بعيش فيها الإنسان، الناس اللي
+
+464
+00:34:42,450 --> 00:34:46,070
+بعيشوا على المرتفعات نظرا لوجود نقص في الـ oxygen
+
+465
+00:34:46,070 --> 00:34:50,890
+جسمهم بيصنع خلايا RBCs بزيادة، تمام؟ هذه حالة
+
+466
+00:34:50,890 --> 00:34:54,050
+polycythemia، صح هم normal بس بيندرجوا تحت الـ
+
+467
+00:34:54,050 --> 00:35:01,370
+polycythemia، تمام؟ والـ .. الناس اللي عندهم organ
+
+468
+00:35:01,370 --> 00:35:05,630
+dysfunction، إذا عند شخص عنده مشكلة بالقلب، القلب
+
+469
+00:35:05,630 --> 00:35:09,690
+هو مضخة الدم، هو اللي بيضخ الدم لـ .. لـ .. لـ سائر
+
+470
+00:35:09,690 --> 00:35:13,630
+أعضاء الجسم، طبعا؟ طيب، فإذا في عندي مشكلة في
+
+471
+00:35:13,630 --> 00:35:16,590
+القلب يعني في عندي مشكلة في ضخ الدم، يعني في عندي
+
+472
+00:35:16,590 --> 00:35:21,900
+مشكلة في توصيل الـ oxygen، طبعا؟ الـ bone marrow اللي
+
+473
+00:35:21,900 --> 00:35:27,340
+بيصنع لي الـ .. الدم مش راح يفهم أن المشكلة في الـ
+
+474
+00:35:27,340 --> 00:35:33,340
+heart، في القلب طبعا؟ لأ، هو بس عنده مجسات بيحس نسبة
+
+475
+00:35:33,340 --> 00:35:37,620
+الـ oxygen، مادام في عندي أي خلل أدى إلى نقص نسبة الـ
+
+476
+00:35:37,620 --> 00:35:41,340
+oxygen في الدم بيبدأ يصنع RBC's وهو مغمض عيناه
+
+477
+00:35:41,340 --> 00:35:49,620
+طبعا؟ يعني هو مصنع ولا بيسأل زي .. يعني مغمض عيناه
+
+478
+00:35:49,990 --> 00:35:54,650
+تمام؟ بيشتغل وهو مغمض عينه، مش شايف من المشكلة بس
+
+479
+00:35:54,650 --> 00:35:59,900
+حاسس أنه ما فيش oxygen، هذا هو الـ bone marrow، نتيجة مشكلة
+
+480
+00:35:59,900 --> 00:36:02,960
+في القلب، يعني في ضخ الدم أو مشكلة في الرئتين، في
+
+481
+00:36:02,960 --> 00:36:08,540
+التنفس، في هذه الحالتين المحصلة hypoxia، إيش يعني
+
+482
+00:36:08,540 --> 00:36:14,380
+hypoxia؟ hypo قليل، oxia أكسجين، تمام؟ فبالتالي
+
+483
+00:36:14,380 --> 00:36:19,500
+المحصلة نقص في الأكسجين في الجسم، فالـ bone marrow
+
+484
+00:36:19,500 --> 00:36:24,170
+كان مصنع بيشتغل وهو مغمض عيناه، بس بيحس في .. في
+
+485
+00:36:24,170 --> 00:36:28,890
+oxygen كافي بيبطل يصنع، مش oxygen كافي بيصير يصنع،
+
+486
+00:36:28,890 --> 00:36:31,850
+عندي مشكلة في القلب أو في الرئتين، راح تخلي الـ
+
+487
+00:36:31,850 --> 00:36:37,270
+bone marrow أنه يحس أنه ما في oxygen كافي وبالتالي
+
+488
+00:36:37,270 --> 00:36:42,750
+معناها أنه راح يصنع خلايا RBCs بزيادة، والمحصلة
+
+489
+00:36:42,750 --> 00:36:47,320
+polycythemia، الحالة الثالثة أو النوع الثالث من الـ
+
+490
+00:36:47,320 --> 00:36:51,740
+polycythemia أنه يكون في عندي tumor للخلايا الأم لـ
+
+491
+00:36:51,740 --> 00:36:55,320
+RBCs اللي موجودة في الـ bone marrow، طبعا كل نوع من
+
+492
+00:36:55,320 --> 00:36:59,940
+أنواع خلايا الدم له خلايا أم في الـ bone marrow، خلايا
+
+493
+00:36:59,940 --> 00:37:05,920
+من .. بس تتكثر بتنتج،  ليه هذا النوع طبعا، الخلايا اللي
+
+494
+00:37:05,920 --> 00:37:09,300
+أم لـ RBCs اللي موجودة في الـ bone marrow صار فيها
+
+495
+00:37:09,300 --> 00:37:13,380
+خلل، طفرة معينة أدت إلى أنها بتتكاثر بشكل مجنون
+
+496
+00:37:13,380 --> 00:37:17,680
+فبالتالي المحصلة إنتاج المزيد من الـ RBCs above
+
+497
+00:37:17,680 --> 00:37:20,740
+normal، أكثر من أعلى من الـ normal، فيعني
+
+498
+00:37:20,740 --> 00:37:24,960
+polycythemia، إذا هي مجموعة من الـ disorders وكلها
+
+499
+00:37:24,960 --> 00:37:28,700
+بندرجها تحت هذا الاسم، تمام؟ فهي معنى كلمة
+
+500
+00:37:28,700 --> 00:37:31,860
+polycythemia، اللي بدي أكون تعرفوا أن hematocrite
+
+501
+00:37:31,860 --> 00:37:37,880
+lower than normal anemia، hematocrite above than
+
+502
+00:37:37,880 --> 00:37:42,580
+normal or more than normal polycythemia
+
+503
+00:37:45,160 --> 00:37:49,460
+طيب فالـ .. إذا هذا هي .. هاي .. هذا تفسير الصورة
+
+504
+00:37:49,460 --> 00:37:54,320
+طيب أول خطوة أو أول نقطة في source of error and
+
+505
+00:37:54,320 --> 00:37:58,540
+comments بيحكي لي عن الـ improper sealing، يعني لو أنا
+
+506
+00:37:58,540 --> 00:38:01,720
+ما سكرتش كويس الـ  capillary وكل شوية .. من الشوية
+
+507
+00:38:01,720 --> 00:38:05,360
+كنا عم نحكي عنها، حكينا إنه إيش راح يصير؟ راح يصير
+
+508
+00:38:05,360 --> 00:38:09,300
+فيه decrease للـ hematocrit، why؟ لأنه في عندي loss
+
+509
+00:38:09,300 --> 00:38:13,660
+للـ blood during centrifugation، why؟ لأنه احنا
+
+510
+00:38:13,660 --> 00:38:18,260
+ما سكرناش منيح، فأثناء الطرد المركزي صار في عندي طرد
+
+511
+00:38:18,260 --> 00:38:22,620
+للخلايا باتجاه .. باتجاه منطقة مش مسكرة منيح، يعني
+
+512
+00:38:22,620 --> 00:38:27,360
+تسريب للـ blood، ونظرا لأن الخلايا RBCs هي اللي
+
+513
+00:38:27,360 --> 00:38:32,080
+بتروح بهذا الاتجاه لأنها هي أثقل فاحنا بنفقد RBCs
+
+514
+00:38:32,080 --> 00:38:36,780
+أكثر من الـ plasma، فطالما أنا بقيس القراءة عندي
+
+515
+00:38:36,780 --> 00:38:39,460
+تبع نسبة الـ RBCs إذا راح يكون في عندي
+
+516
+00:38:39,460 --> 00:38:43,140
+decreased hematocrit، إذن a higher number of
+
+517
+00:38:43,140 --> 00:38:46,980
+erythrocytes are lost in relation to the plasma
+
+518
+00:38:46,980 --> 00:38:51,140
+نسبة الـ RBCs اللي أنا بفقدها بالنسبة للبلازما أكثر
+
+519
+00:38:51,140 --> 00:38:56,300
+لأنها هي الأثقل، هي اللي بتتجه باتجاه من .. باتجاه
+
+520
+00:38:56,300 --> 00:38:59,960
+الـ .. المنطقة اللي .. اللي المفروض تكون مسكرة، إذا
+
+521
+00:38:59,960 --> 00:39:03,320
+مش مسكرة منيح راح يصير فقدان لهذه الـ erythrocytes
+
+522
+00:39:03,320 --> 00:39:10,150
+إذا أنا عم بقرأ نسبة وفيها فاقد، تمام؟ طيب an
+
+523
+00:39:10,150 --> 00:39:13,870
+increased amount of anticoagulant decreased the
+
+524
+00:39:13,870 --> 00:39:16,670
+hematocrit reading as a result of erythrocyte
+
+525
+00:39:16,670 --> 00:39:21,110
+shrinkage، إيش معنى الكلام هذا؟ احنا لاحظتوا إنه
+
+526
+00:39:21,110 --> 00:39:27,170
+أنا ركزت كثير أول المحاضرة على موضوع أنه إذا بأخذ
+
+527
+00:39:27,170 --> 00:39:32,130
+عينة من skin puncture على طول مباشرة بأخذ
+
+528
+00:39:32,130 --> 00:39:36,050
+الـ heparinized capillary، الـ red top أو الـ red cap
+
+529
+00:39:36,760 --> 00:39:42,240
+capillary، إذا أنا بأخذ عينة من وريد والعينة
+
+530
+00:39:42,240 --> 00:39:45,740
+فرقتها في إيدي tube بعدين بدي أعبي capillary، بدي
+
+531
+00:39:45,740 --> 00:39:50,020
+أملأ capillary من هاي الـ EDTA tube، طبعا فالمفروض
+
+532
+00:39:50,020 --> 00:39:54,880
+أن الـ .. الـ .. الـ capillary هاي تكون plain، يعني
+
+533
+00:39:54,880 --> 00:40:01,920
+blue cap أو blue top، طبعا، وحكيت لكم إنه ممنوع أستخدم
+
+534
+00:40:01,920 --> 00:40:06,280
+الهيبارين من tube  EDTA، ممنوع أمرر البلد على
+
+535
+00:40:06,280 --> 00:40:10,080
+نوعين من الـ anticoagulants لأن الـ anticoagulants
+
+536
+00:40:10,080 --> 00:40:14,680
+هذه عبارة عن أملاح والأملاح هاي بتأثر على الـ
+
+537
+00:40:14,680 --> 00:40:18,970
+osmolality والـ osmotic pressure للخلايا، طبعا
+
+538
+00:40:18,970 --> 00:40:23,950
+خلينا بس نشوف مع بعض هذه الصورة لحتى تتضح الصورة
+
+539
+00:40:23,950 --> 00:40:32,410
+أكثر لحتى تتضح الصورة، عندي خلايا دم موجودة في وسط أو
+
+540
+00:40:32,410 --> 00:40:37,090
+في محلول بسميه hypertonic، إيش يعني hypertonic؟
+
+541
+00:40:37,090 --> 00:40:41,590
+يعني نسبة الأملاح فيها عالية، طبعا؟ يعني blood
+
+542
+00:40:41,590 --> 00:40:46,130
+موجود في EDTA وهيبارين كمان، نسبة الأملاح عالية
+
+543
+00:40:46,130 --> 00:40:51,250
+حوالين الخلايا، فاحنا عارفين أن الـ .. الـ water اللي
+
+544
+00:40:51,250 --> 00:40:56,430
+موجود داخل هذه الخلايا .. الـ water اللي موجود داخل
+
+545
+00:40:56,430 --> 00:41:02,850
+هذه الخلايا، عارفين إنه بينتقل من الضغط الاسموزي
+
+546
+00:41:02,850 --> 00:41:07,250
+القليل للضغط الاسموزي العالي، يعني من تركيز أملاح
+
+547
+00:41:07,250 --> 00:41:12,370
+قليل لتركيز أملاح عالي فأصبح الـ water هذا الماء
+
+548
+00:41:12,370 --> 00:41:16,050
+اللي موجود داخل الخلايا إيش راح يصير فيه؟ راح
+
+549
+00:41:16,050 --> 00:41:21,090
+ينتقل أو يخرج من الخلايا لحتى يحاول إنه يعادل أو
+
+550
+00:41:21,090 --> 00:41:25,130
+يخفف نسبة الأملاح اللي موجودة حوالين الخلايا اللي
+
+551
+00:41:25,130 --> 00:41:30,960
+هي الـ EDTA والـ heparin مع بعض، طبعا؟ فالمحصلة الخلية
+
+552
+00:41:30,960 --> 00:41:35,520
+لما بتفقد ماء إيش بتصير؟ بتكرمش طبعا؟ فيصير فيها
+
+553
+00:41:35,520 --> 00:41:40,520
+shrinkage، بيصير فيها حاجة اسمها shrinkage طبعا؟
+
+554
+00:41:40,520 --> 00:41:45,100
+الخلية لو كان ضغط الأملاح أو ضغط الاسموزي أو نسبة
+
+555
+00:41:45,100 --> 00:41:51,180
+الأملاح حوالين الخلايا مساوية لـ .. الـ .. الـ .. نسبة
+
+556
+00:41:51,180 --> 00:41:56,240
+الأملاح داخل الخلايا، إذا الماء هيكون حركته منتظمة
+
+557
+00:41:56,240 --> 00:42:01,080
+من وإلى الخلية، إذا الخلية هتبقى intact، سليمة،
+
+558
+00:42:01,080 --> 00:42:04,200
+تمام؟ intact يعني كلمة .. كلمة intact معناها أن
+
+559
+00:42:04,200 --> 00:42:10,330
+الخلايا سليمة، بالشكل السليم، تمام؟ لو كان في عندي الـ
+
+560
+00:42:10,330 --> 00:42:13,970
+.. الـ .. طبعا هذا المحلول اللي بيكون فيه انتظام في
+
+561
+00:42:13,970 --> 00:42:19,610
+نسبة الأملاح أو نسبة الأملاح فيه مساوية لتركيزها داخل
+
+562
+00:42:19,610 --> 00:42:27,020
+الخلايا بسميه isotonic solution، تمام؟ طيب، محلول نسبة
+
+563
+00:42:27,020 --> 00:42:34,080
+الأملاح فيه أقل من داخل الخلايا بسميه hypotonic، إذا
+
+564
+00:42:34,080 --> 00:42:39,060
+الأملاح خارج الخلايا أكثر من داخلها hypertonic
+
+565
+00:42:39,060 --> 00:42:44,340
+أكثر، hyper، تمام؟ محلول نسبة الأملاح خارج الخلايا
+
+566
+00:42:44,340 --> 00:42:50,260
+مساوية لداخلها بسميه isotonic solution، محلول نسبة
+
+567
+00:42:50,260 --> 00:42:55,500
+الأملاح فيه أقل من داخل الخلايا أو الوسط يعني
+
+568
+00:42:55,500 --> 00:43:01,140
+الوسط للخلية في نسبة الأملاح أقل من داخلها بسميها
+
+569
+00:43:01,140 --> 00:43:07,260
+hypotonic، أقل، hypo، طيب، مادام نسبة الأملاح حوالين
+
+570
+00:43:07,260 --> 00:43:11,060
+الخلايا أقل، معناها كيف هتصير حركة الماء؟ هتصير
+
+571
+00:43:11,060 --> 00:43:14,120
+حركة الماء من التركيز الأقل إلى داخل الخلايا،
+
+572
+00:43:14,120 --> 00:43:18,920
+التركيز الأعلى هيكون هو داخل الخلايا، تمام؟ فإنه
+
+573
+00:43:18,920 --> 00:43:26,720
+نظرا لأن الماء بيتحرك إلى داخل الخلية، معناها إنه
+
+574
+00:43:26,720 --> 00:43:31,320
+ممكن يدخل إلى الخلية ماء أكثر من طاقتها
+
+575
+00:43:31,320 --> 00:43:36,000
+الاستيعابية، مما يؤدي إلى إيش؟ انفجار الخلايا،
+
+576
+00:43:36,000 --> 00:43:42,600
+lysis للخلايا، تكسر، breakdown للخلايا، تمام؟
+
+577
+00:43:42,600 --> 00:43:47,820
+طيب، هاي في عندي كمان صورة ثانية لحتى تتضح الفكرة
+
+578
+00:43:47,820 --> 00:43:53,510
+أكثر، طبعا هاي محلول hypertonic، إيش يعني؟ يعني
+
+579
+00:43:53,510 --> 00:43:57,850
+الأملاح حوالين الخلايا .. هاي الخلايا اللي لونها
+
+580
+00:43:57,850 --> 00:44:02,470
+أحمر، الأملاح حوالين الخلايا أكثر من داخلها طبعا
+
+581
+00:44:02,470 --> 00:44:06,170
+أكثر من داخلها، شايفين؟ هاي الدائرة هذه .. هاي
+
+582
+00:44:06,170 --> 00:44:09,730
+الدائرة، شايفين؟ فيها نسبة أملاح اللي لونهم أزرق
+
+583
+00:44:09,730 --> 00:44:14,130
+هدول طبعا أكثر من الأملاح اللي موجودة داخل الخلية
+
+584
+00:44:14,130 --> 00:44:23,810
+إيش معناها بدو يصير يا بنات؟ معناها بدو الـ .. الـ ..
+
+585
+00:44:23,810 --> 00:44:28,810
+إيش اللي بدو يصير؟ بدو يصير عندي shrinkage، تكرمش
+
+586
+00:44:28,810 --> 00:44:32,490
+للخلية، هذه المصطلحات سيبكم منها طبعا، سيبكم منها
+
+587
+00:44:32,490 --> 00:44:35,270
+هذه المصطلحات، هلأ أنا هكتب لكم إيش يعني كلمة
+
+588
+00:44:35,270 --> 00:44:39,790
+shrinkage وإيش يعني intact و .. و .. و lysis
+
+589
+00:44:39,790 --> 00:44:45,370
+بس المهم الفكرة، طيب هي هذه الدائرة نسبة الأملاح
+
+590
+00:44:45,370 --> 00:44:52,460
+فيها أقل من اللي داخل الخلية، يعني hypotonic، ماشي،
+
+591
+00:44:52,460 --> 00:44:55,500
+معناها الـ .. الـ .. الخلية إيش راح يصير؟ راح تنتفخ،
+
+592
+00:44:55,500 --> 00:44:58,960
+تنتفخ، شايفين؟ هي انتفخت، تنتفخ إلى أن
+
+593
+00:44:58,960 --> 00:45:03,540
+يصل فيها الماء أكثر من طاقتها الاستيعابية وبتقوم
+
+594
+00:45:03,540 --> 00:45:09,460
+.. بتنفجر، تمام؟ طيب isotonic يعني تركيز الأملاح في
+
+595
+00:45:09,460 --> 00:45:12,960
+.. حوالين الخلايا مساوي لتركيزها في الداخل فهي
+
+596
+00:45:12,960 --> 00:45:17,880
+يعني awesome، رائع، wow، تمام؟ intact الخلية، طيب،
+
+597
+00:45:17,880 --> 00:45:23,760
+خلينا بس نلخص الشيء عشان أورجيكم الـ .. الكلمات
+
+598
+00:45:23,760 --> 00:45:27,520
+يعني لو كانت الـ .. الـ .. الخلايا موجودة في
+
+599
+00:45:27,520 --> 00:45:31,280
+isotonic تمام؟ solution
+
+600
+00:45:35,280 --> 00:45:41,480
+معناها إيش رح يكون؟ رح تكون intact، سليمة، يعني
+
+601
+00:45:41,480 --> 00:45:46,960
+تركيز الأملاح حواليها مساوي لتركيز الأملاح داخلها،
+
+602
+00:45:46,960 --> 00:45:51,260
+حركة الماء منتظمة من وإلى الخلية، الخلية هتظل
+
+603
+00:45:51,260 --> 00:45:58,060
+intact، طيب، لو كان في عندي محلول hypertonic، تمام؟
+
+604
+00:45:58,060 --> 00:46:00,140
+hypertonic solution
+
+605
+00:46:04,350 --> 00:46:08,870
+hypertonic solution، إيش معناها؟ معناها تركيز
+
+606
+00:46:08,870 --> 00:46:13,210
+الأملاح حوالين الخلايا أكثر من تركيز الأملاح داخلها
+
+607
+00:46:13,210 --> 00:46:19,870
+هذا إيش رح يؤدي؟ رح يؤدي إلى أن الماء رح يخرج من
+
+608
+00:46:19,870 --> 00:46:25,970
+الخلية إلى خارجها، يعني المحصلة أن الخلية فقدت سائل
+
+609
+00:46:25,970 --> 00:46:32,510
+فقدت سائل، إيش يعني؟ رح يصير فيها shrinkage، رح يصير
+
+610
+00:46:32,510 --> 00:46:38,830
+فيها تكرمش، طيب لو كان في عندي محلول صار فيه إيه؟ هو
+
+611
+00:46:38,830 --> 00:46:47,670
+عبارة عن hypotonic .. hypotonic solution، تمام؟
+
+612
+00:46:49,230 --> 00:46:53,170
+معناها هذا المحلول أو الوسط المحيط بالخلايا يا
+
+613
+00:46:53,170 --> 00:46:55,910
+بنات، سواء محلول أو وسط المحيط، يعني نفس المعنى
+
+614
+00:46:55,910 --> 00:46:59,790
+انتوا تخيلوها الوسط المحيط بالخلايا hypotonic
+
+615
+00:4
+
+667
+00:51:13,220 --> 00:51:18,260
+هيك بإيدي أو أحاول أبطئ سرعته، why؟ لإنه حكينا ال
+
+668
+00:51:18,260 --> 00:51:24,520
+.. ال .. يعني ممنوع أن أجبره إنه يقف من خلال
+
+669
+00:51:24,520 --> 00:51:27,100
+applying pressure to the metal cover plate، عندي أنا
+
+670
+00:51:27,100 --> 00:51:32,870
+أحط إيدي أو يعني أعمل ضغط على الـ .. الغطاء، هذا
+
+671
+00:51:32,870 --> 00:51:37,030
+will cause، ليش حكينا؟ لإنه هيخلي الـ RBCs، ليه؟ طبقة
+
+672
+00:51:37,030 --> 00:51:41,070
+الـ RBCs اللي اترسبت راح يصير لها sling، تنفصل باتجاه
+
+673
+00:51:41,070 --> 00:51:43,810
+الـ plasma، طبعا راح يصير لها forward إلى الأمام
+
+674
+00:51:43,810 --> 00:51:47,890
+باتجاه الـ plasma، و الـ result راح ينتج عنها إيه؟
+
+675
+00:51:47,890 --> 00:51:51,530
+أنه falsely elevated، elevated، يعني increased، طبعا
+
+676
+00:51:51,530 --> 00:51:56,750
+result، طيب if too much time elapses between when
+
+677
+00:51:56,750 --> 00:51:59,170
+the centrifuge stops and the capillary tube is
+
+678
+00:51:59,170 --> 00:52:04,890
+removed، هلأ إذا في وقت طويل ما بين توقف الـ
+
+679
+00:52:04,890 --> 00:52:08,030
+centrifuge ولما أنا روحت أفضيت و روحت أقرأ
+
+680
+00:52:08,030 --> 00:52:12,110
+النتيجة، هذا أيضا ممكن يأثر على النتائج، لإنه في هذا
+
+681
+00:52:12,110 --> 00:52:18,630
+الوقت ممكن تكون الـ RBCs بدأت تتحلل، بدأت يعني يبطل
+
+682
+00:52:18,630 --> 00:52:26,290
+ترسيبها أو يعني sedimentation، تمام؟ فبالتالي ممكن أن
+
+683
+00:52:26,290 --> 00:52:29,990
+تتحرك أيضا باتجاه البلازما، وهذا ممكن يعملي falsely
+
+684
+00:52:29,990 --> 00:52:32,010
+elevated hematocrit، تمام؟
+
+685
+00:52:35,890 --> 00:52:39,130
+The buffy coat of a specimen shouldn't be included
+
+686
+00:52:39,130 --> 00:52:43,070
+أنا مش لازم أقرأ وأضمن الـ buffy coat من ضمن
+
+687
+00:52:43,070 --> 00:52:45,670
+القراءة، حكينا بقرأ من تحت الـ .. الـ .. الـ buffy
+
+688
+00:52:45,670 --> 00:52:47,890
+coat، shouldn't be included with the reading
+
+689
+00:52:47,890 --> 00:52:51,750
+because it's inclusion falsely elevates the result
+
+690
+00:52:51,750 --> 00:52:55,570
+يعني القراءة تبعته، لو أنا بدي أدخل الـ buffy coat
+
+691
+00:52:55,570 --> 00:52:58,890
+في القراءة تبعتي، هذا هيرفع لي الـ .. الـ hematocrit
+
+692
+00:52:58,890 --> 00:53:02,980
+وهذا خطأ، a decrease or increase in the reading may
+
+693
+00:53:02,980 --> 00:53:06,320
+be seen if the microhematocrit reader is not used
+
+694
+00:53:06,320 --> 00:53:09,840
+properly، إذا أنا ما استخدمت التدريج صح ممكن أيضا
+
+695
+00:53:09,840 --> 00:53:12,160
+يكون عندي فيه error في النتيجة، decrease or
+
+696
+00:53:12,160 --> 00:53:18,210
+increase، تمام؟ ركزوا معايا في النقطة رقم 9، في عندي
+
+697
+00:53:18,210 --> 00:53:23,870
+أمراض، a number of disorders، عدد من الأمراض زي الـ
+
+698
+00:53:23,870 --> 00:53:27,310
+sickle cell anemia، انيميا الخلايا المنجلية، اللي
+
+699
+00:53:27,310 --> 00:53:30,630
+بيكون فيها الخلايا الـ RBCs ماخدة شكل الـ .. الـ ..
+
+700
+00:53:30,630 --> 00:53:33,810
+الـ .. منجل، شكل الـ .. الـ .. الـ .. الـ .. الـ ..
+
+701
+00:53:33,810 --> 00:53:33,990
+الـ .. الـ .. الـ .. الـ .. الـ .. الـ .. الـ .. الـ
+
+702
+00:53:33,990 --> 00:53:34,290
+.. الـ .. الـ .. الـ .. الـ .. الـ .. الـ .. الـ .. الـ
+
+703
+00:53:34,290 --> 00:53:34,630
+الـ .. الـ .. الـ .. الـ .. الـ .. الـ .. الـ .. الـ ..
+
+704
+00:53:34,630 --> 00:53:34,950
+.. الـ .. الـ .. الـ .. الـ .. الـ .. الـ .. الـ .. الـ ..
+
+705
+00:53:34,950 --> 00:53:36,030
+الـ .. الـ .. الـ .. الـ .. الـ .. الـ .. الـ .. الـ ..
+
+706
+00:53:36,030 --> 00:53:38,790
+.. الـ .. الـ .. الـ .. الـ .. الـ .. الـ .. الـ .. الـ ..
+
+707
+00:53:38,790 --> 00:53:38,850
+الـ .. الـ .. الـ .. الـ .. الـ .. الـ .. الـ .. الـ ..
+
+708
+00:53:38,850 --> 00:53:42,450
+.. الـ .. الـ .. الـ .. الـ .. الـ ..
+
+709
+00:53:46,230 --> 00:53:49,470
+hypochromic anemia، الخلايا اللي بيكون فيها مشكلة
+
+710
+00:53:49,470 --> 00:53:51,750
+في الـ hemoglobin، يعني الخلايا بتكون فيها صغيرة
+
+711
+00:53:51,750 --> 00:53:55,930
+أصغر من الطبيعي، spherocytosis، بتكون الخلايا عاملة
+
+712
+00:53:55,930 --> 00:53:59,570
+زي شكل الكرة، مش discocytic و biconcave، احنا أخدنا
+
+713
+00:53:59,570 --> 00:54:03,950
+أن الـ RBCs، إذا طلعت عليها نظرة من فوق، بشوفها
+
+714
+00:54:03,950 --> 00:54:09,630
+discocytic، side view بشوفها biconcave، صح؟ طيب،
+
+715
+00:54:09,630 --> 00:54:14,530
+فإذا الـ .. الـ .. لما أجي أنا أطلع عليها من النظرة
+
+716
+00:54:14,530 --> 00:54:17,870
+الجانبية، ما بشوفهاش biconcave، بشوفها دائرة، يعني
+
+717
+00:54:17,870 --> 00:54:23,280
+هذه ماخدة شكل الكرة، spherocytosis، ثلاثية مرض
+
+718
+00:54:23,280 --> 00:54:27,020
+الأنيميا البحر المتوسط، وكلنا سمعنا عن الثلاثيميا
+
+719
+00:54:27,020 --> 00:54:32,160
+ومشكلة الثلاثيميا إنه بيكون في تباين في شكل الـ
+
+720
+00:54:32,160 --> 00:54:39,460
+RBCs، طبعا هدول بينتج عنها الخلل في شكل الـ RBCs، انيميا
+
+721
+00:54:39,460 --> 00:54:43,340
+خلايا منجلية، Sickle Cell anemia، يعني الـ RBCs زي
+
+722
+00:54:43,340 --> 00:54:49,570
+شكل المنجل، macrocytic anemia، خلايا كبيرة، وحجم
+
+723
+00:54:49,570 --> 00:54:53,350
+الخلايا كبيرة، يعني الخلية نفسها كبيرة، hypochromic
+
+724
+00:54:53,350 --> 00:54:57,030
+anemia، الخلايا .. الخلية صغيرة، spherocytosis
+
+725
+00:54:57,030 --> 00:55:00,330
+الخلية مش ماخدة شكل طبيعي، مش discocytic ولا biconcave
+
+726
+00:55:00,330 --> 00:55:07,340
+ماخدة شكل كروي، تمام؟ ثلاثية، تباين في شكل الخلية هذا
+
+727
+00:55:07,340 --> 00:55:11,860
+راح يودي أثناء الـ .. الـ .. الفصل، راح يودي أثناء
+
+728
+00:55:11,860 --> 00:55:15,160
+الفصل، أثناء الـ centrifugation، إلى إنه جزء من اللي
+
+729
+00:55:15,160 --> 00:55:20,800
+بلازما يصير له trapped، ينحجز بين الخلايا، وهذا راح
+
+730
+00:55:20,800 --> 00:55:25,300
+يأثر على القراءة تبعتي، طيب عشان نفهم الفكرة هذه، أنا
+
+731
+00:55:25,300 --> 00:55:31,040
+جبت لكم صورة عبارة عن كرات، تمام؟ خلينا لو نشوف
+
+732
+00:55:31,040 --> 00:55:35,260
+أنا عندي سلة و بدي أصفّ فيها الكرات، وتكون
+
+733
+00:55:35,260 --> 00:55:40,300
+الكرات بشكل منتظم، تمام؟ ونفس الحجم ونفس الشكل
+
+734
+00:55:40,300 --> 00:55:45,620
+سهل جدا أصفّها بين بعضها، تمام؟ لو جيت هذه الكرات
+
+735
+00:55:45,620 --> 00:55:48,540
+كبّيتها في .. في داخل السلة أو في داخل الـ .. الـ .. الـ
+
+736
+00:55:48,540 --> 00:55:52,040
+container، خلينا نفترض أنها هي داخل صندوق وحطيت
+
+737
+00:55:52,040 --> 00:55:57,400
+صفّتها بشكل منتظم على بعضها البعض وجيت أكبّ عليها
+
+738
+00:55:57,400 --> 00:56:03,060
+مثلا سائل، ماء، نسبة الماء اللي ممكن يحتجز داخل أو
+
+739
+00:56:03,060 --> 00:56:06,760
+ما بين الخلايا هتكون قليلة لإنه منتظمة، لإنه ما فيش
+
+740
+00:56:06,760 --> 00:56:15,980
+فراغات ما بينها، تمام؟ ونفس الفكرة هنا شايفين؟
+
+741
+00:56:15,980 --> 00:56:21,260
+هذه من لعبة، ماخذينها من لعبة الكرات، تمام؟ بس أنا
+
+742
+00:56:21,260 --> 00:56:25,420
+أخذت الفكرة منها، اتخيلي أن هذه الـ RBCs
+
+743
+00:56:25,420 --> 00:56:29,060
+تبعتي كلها نفس الشكل وتقريبا كلها لونها أحمر،
+
+744
+00:56:29,060 --> 00:56:32,910
+يعني كلها red blood cells، تمام؟ لو أنا جيت داخل الـ
+
+745
+00:56:32,910 --> 00:56:36,450
+capillary tube وبعد ما عملت لها centrifugation
+
+746
+00:56:36,450 --> 00:56:39,710
+صار لها sedimentation، صارت تترسب على بعضها بشكل
+
+747
+00:56:39,710 --> 00:56:44,230
+regular، تمام؟ بشكل طبعا هيكون المسافات ما بينها
+
+748
+00:56:44,230 --> 00:56:49,410
+قليلة جدا، يعني البلازما يا بنات مش راح تنحجز بينها
+
+749
+00:56:49,410 --> 00:56:55,030
+مش راح تنحجز بينها، تمام؟ فإذا أنا النسبة اللي
+
+750
+00:56:55,030 --> 00:56:59,430
+بقرأها هذه .. نسبة اللي بقرأها .. نسبة الخلايا هي
+
+751
+00:56:59,430 --> 00:57:04,070
+فقط خلايا، مش خلايا وبلازما، صح؟ يعني الـ hematocrit
+
+752
+00:57:04,070 --> 00:57:09,370
+هيكون بيعبر لي بشكل أدق عن نسبة الـ RBCs لو كانت
+
+753
+00:57:09,370 --> 00:57:15,080
+الخلايا منتظمة ونفس الشكل، لكن لو كانت الخلايا منها
+
+754
+00:57:15,080 --> 00:57:20,600
+.. منها زي هيك .. زي هذا الشكل، تمام؟ وهي بينها
+
+755
+00:57:20,600 --> 00:57:24,520
+المنجل، الخلايا اللي .. اللي عاملة .. ماخدة شكل
+
+756
+00:57:24,520 --> 00:57:27,960
+المنجل، الخلايا المنجلية أو خلايا كبيرة، وخلايا
+
+757
+00:57:27,960 --> 00:57:31,120
+صغيرة في الـ macrocytic أو خلايا صغيرة وخلايا
+
+758
+00:57:31,120 --> 00:57:35,300
+طبيعية في الـ hypochromic أو خلايا ماخدة شكل قرصي أو
+
+759
+00:57:35,300 --> 00:57:38,680
+biconcave، وخلايا ماخدة شكل كرة أو أن في عندي
+
+760
+00:57:38,680 --> 00:57:45,160
+تباين في أشكال الخلايا، زي هذا بالظبط، خلينا بس نعرض
+
+761
+00:57:45,160 --> 00:57:51,520
+الصورة هاي، نشوف، افترض أن هدول خلايا RBCs متباينة
+
+762
+00:57:51,520 --> 00:57:55,200
+الشكل، إيش صغير، إيش كبير، إيش كروي، إيش بيضاوي، إيش
+
+763
+00:57:55,200 --> 00:58:00,000
+منجل، وبدك تحطيهم في container وتفصلّيهم مع بعض
+
+764
+00:58:00,000 --> 00:58:04,290
+عشان تاخدي الحجم، هيكون طبعا مش هيجلسوا على بعض، مش
+
+765
+00:58:04,290 --> 00:58:08,010
+هيتصفّوا على بعض بانتظام، فبالتالي هيكون في فراغات
+
+766
+00:58:08,010 --> 00:58:12,070
+ما بينهم، سهلة الفراغات هتتعبيها البلازما، إذا أنا
+
+767
+00:58:12,070 --> 00:58:15,230
+لما بدي أقرأ نسبة الخلايا، الـ volume اللي بتحتله
+
+768
+00:58:15,230 --> 00:58:21,110
+هذه الخلايا المتباينة، هكون بقرأ RBCs وبيتلخللها
+
+769
+00:58:21,110 --> 00:58:23,910
+بلازما، إذا نسبة الـ hematocrit تبعتي يا بنات
+
+770
+00:58:23,910 --> 00:58:31,090
+إشمالها هتكون خطأ، نتيجة إيش؟ وجود trapped plasma
+
+771
+00:58:31,090 --> 00:58:35,250
+طبعا، الـ plasma to be trapped، الـ plasma انحجزت
+
+772
+00:58:35,250 --> 00:58:38,610
+بين الخلايا، الأفضل أن تكون الخلايا منتظمة عشان
+
+773
+00:58:38,610 --> 00:58:41,130
+ما يكونش في بينها فراغات وما يصير لل plasma ما تصير
+
+774
+00:58:41,130 --> 00:58:46,430
+لها trapping، The trapping of plasma يعني أنه ينحجز
+
+775
+00:58:46,430 --> 00:58:50,290
+للبلازما داخل أو ما بين، في المسافات البينية بين الـ
+
+776
+00:58:50,290 --> 00:58:54,650
+RBCs غير منتظمة، هذا ممكن يخلي أن الـ hematocrit
+
+777
+00:58:54,650 --> 00:58:59,890
+تبعتي يكون 1 إلى 3% higher than the obtained of the
+
+778
+00:58:59,890 --> 00:59:03,280
+automated instrument، طبعا يعني لو أنا حطيته على
+
+779
+00:59:03,280 --> 00:59:09,060
+جهاز .. جهاز الـ CBC وحسبت hematocrit طبعا، وقرأته
+
+780
+00:59:09,060 --> 00:59:11,940
+بالتجربة اللي احنا شرحناها، بتجربة الـ micro
+
+781
+00:59:11,940 --> 00:59:15,240
+hematocrit، هلاحظ أن التجربة بتعطيني نسبة
+
+782
+00:59:15,240 --> 00:59:18,520
+hematocrit أعلى من 1 إلى 3%، يعني لو جهاز الـ CBC
+
+783
+00:59:18,520 --> 00:59:25,620
+أعطاني القيمة خمسة وثلاثين، تمام؟ على جهاز الـ CBC
+
+784
+00:59:25,620 --> 00:59:30,160
+ممكن القراءة تبعتي باستخدام الـ capillary tube
+
+785
+00:59:30,160 --> 00:59:33,100
+تكون ستة وثلاثين، أو سبعة وثلاثين، أو ثمانية و
+
+786
+00:59:33,100 --> 00:59:39,930
+ثلاثين، طبعا من 1 إلى 3% أعلى، ليش؟ لإنه في نسبة trapped
+
+787
+00:59:39,930 --> 00:59:44,870
+plasma، وأنا لما قرأت قرأت الـ RBCs الغير منتظمة
+
+788
+00:59:44,870 --> 00:59:49,230
+هذه والـ plasma المحجوزة بينها، إذا زادت نسبة الـ
+
+789
+00:59:49,230 --> 00:59:52,550
+hematocrit عندي، فالأفضل أن الناس اللي عندهم
+
+790
+00:59:52,550 --> 00:59:56,290
+الأمراض هذه ما أشتغل لهم الـ hematocrit بطريقة الـ
+
+791
+00:59:56,290 --> 01:00:01,350
+capillary tube، أشتغله بجهاز الـ CBC لإنه جهاز الـ CBC
+
+792
+01:00:01,350 --> 01:00:05,290
+بيعمله .. بيعمله calculation وليس measurement
+
+793
+01:00:05,290 --> 01:00:12,190
+بيحسب الـ .. الـ hematocrit من الـ .. من حجم الـ RBC و
+
+794
+01:00:12,190 --> 01:00:17,470
+عددها، تمام؟ يعني بيطبق قانون معين وبعدين بي ..
+
+795
+01:00:17,470 --> 01:00:21,690
+بيحسبه، ليه؟ نسبة الـ hematocrit إذا ما بتأثر قيمة الـ
+
+796
+01:00:21,690 --> 01:00:24,890
+hematocrit اللي جاية من الجهاز، من جهاز الـ CBC، ما
+
+797
+01:00:24,890 --> 01:00:30,030
+بتتأثر بالـ trapped plasma، طيب فإذا هذه فكرة trapped
+
+798
+01:00:30,030 --> 01:00:33,310
+plasma، الناس اللي عندهم الأمراض هدول بقرأ لهم على
+
+799
+01:00:33,310 --> 01:00:36,850
+جهاز الـ CBC، ما بشتغل بطريقة micro hematocrit tube،
+
+800
+01:00:36,850 --> 01:00:40,910
+capillary tube، طيب a temporarily low hematocrit
+
+801
+01:00:40,910 --> 01:00:45,490
+reading may result immediately after blood loss، لو
+
+802
+01:00:45,490 --> 01:00:51,310
+شخص عنده نزيف، شخص عنده blood loss، معناها راح يكون
+
+803
+01:00:51,310 --> 01:00:54,530
+في عنده low hematocrit، نتيجة فقدان الـ .. الـ .. الـ
+
+804
+01:00:54,530 --> 01:01:03,140
+blood، نتيجة فقدان الـ .. الخلايا، أول شيء السبب في
+
+805
+01:01:03,140 --> 01:01:06,200
+الـ low hematocrit إنه فقد خلايا، شخص عنده blood
+
+806
+01:01:06,200 --> 01:01:12,240
+loss، ثاني سبب، إنه الجسم لما بده يعود
+
+807
+01:01:12,240 --> 01:01:17,720
+بيعود للـ plasma أسرع ما بيعود للخلايا، فبيكون في
+
+808
+01:01:17,720 --> 01:01:22,860
+عندي لفترة مؤقتة بعد النزيف، حتى لو توقف، هيضل إنه
+
+809
+01:01:22,860 --> 01:01:26,140
+في عنده لفترة مؤقتة، الـ hematocrit هيضل له إلى أن
+
+810
+01:01:26,140 --> 01:01:29,300
+الجسم لبين ما يأخذ وقته في تعويض الـ erythrocyte
+
+811
+01:01:29,300 --> 01:01:35,000
+اللي تم فقدانها، طيب proper specimen collection is
+
+812
+01:01:35,000 --> 01:01:38,240
+an important consideration، أن أنا أسحب العينة بشكل صح
+
+813
+01:01:38,240 --> 01:01:42,960
+يا بنات، برضه كمان بيأثر على النتيجة، اتخيلي سحبتي
+
+814
+01:01:42,960 --> 01:01:46,240
+من منطقة فيها hematoma، يعني سحبتي hemolyzed blood
+
+815
+01:01:46,240 --> 01:01:49,880
+blood، مكسر، يعني الـ hematoma هيصير له decreased
+
+816
+01:01:49,880 --> 01:01:56,320
+اتخيلي أنتي سحبتي الـ .. الـ .. الـ .. الـ .. الـ tube ..
+
+817
+01:01:56,320 --> 01:02:00,880
+الـ blood وما فضّيتيه كويس في الـ tube، فمعناها صار في
+
+818
+01:02:00,880 --> 01:02:03,860
+clot، يعني في صار عندي consuming في الـ erythrocytes
+
+819
+01:02:03,860 --> 01:02:08,860
+معناها هتتأثر النتيجة، تخيلي أنتي سحبتي العينة و
+
+820
+01:02:08,860 --> 01:02:12,620
+حطّيتيها في EDTA tube، بعدين ملقيتيها hyperionized
+
+821
+01:02:12,880 --> 01:02:15,660
+vocabulary، معناها صار في عندي shrinkage في الخلايا
+
+822
+01:02:15,660 --> 01:02:19,980
+نتيجة إنه في عندي double anticoagulant، نوعين من الـ
+
+823
+01:02:19,980 --> 01:02:22,380
+anticoagulant، معناها يصير فيه decrease في الـ
+
+824
+01:02:22,380 --> 01:02:28,020
+hematocrit، إذا طريقة جمع العينة وإجراء الفحص مهمة
+
+825
+01:02:28,020 --> 01:02:33,720
+جدا لحتى أحصل على representative results أو
+
+826
+01:02:33,720 --> 01:02:40,290
+accurate and precise results، نتائج ممثلة، معبرة عن
+
+827
+01:02:40,290 --> 01:02:46,950
+حالة الشخص اللي أنا عم بشتغل له الفحص أو يعني نتائج
+
+828
+01:02:46,950 --> 01:02:50,350
+accurate and precise، صحيحة ودقيقة، the
+
+829
+01:02:50,350 --> 01:02:53,490
+introduction of interstitial fluid from skin، لو
+
+830
+01:02:53,490 --> 01:02:57,390
+أنا اتخيلي بـ .. بدي أسحب عينة، skin puncture
+
+831
+01:02:57,390 --> 01:03:03,090
+ويصير أعيصِر فيها، إيش يعني؟ عصرت فيها، حكينا إنه هيصير
+
+832
+01:03:03,090 --> 01:03:07,150
+في عندي tissue juice، عصارة الخلايا، هتطلع مع الـ
+
+833
+01:03:07,150 --> 01:03:11,810
+blood، يعني أنا بأخذ diluted blood، مع إني أخدت
+
+834
+01:03:11,810 --> 01:03:
+
+889
+01:07:45,010 --> 01:07:48,210
+بيطبقوا قانون التلاتة اللي هو ايش بيحكي قانون
+
+890
+01:07:48,210 --> 01:07:54,490
+التلاتة أنه ال hemoglobin يساوي sorry أنه ال
+
+891
+01:07:54,490 --> 01:07:57,530
+hemoglobin بيساوي ال hematocrit على تلاتة plus
+
+892
+01:07:57,530 --> 01:08:00,690
+minus تلاتة أو ال hematocrit يساوي ال hemoglobin
+
+893
+01:08:00,690 --> 01:08:04,990
+ضارب تلاتة plus minus تلاتة طبعا، وهاي هنا يعطيني
+
+894
+01:08:04,990 --> 01:08:13,380
+مثال لو كان عندك أنه ال hemoglobin 12 طبعا، فهي تطبق
+
+895
+01:08:13,380 --> 01:08:16,560
+في قانون ال hematocrit في القانون هذا هي اللي
+
+896
+01:08:16,560 --> 01:08:23,340
+هو 12 في 3 = 36 طبعا، اذا ال hematocrit 36 أو هو plus
+
+897
+01:08:23,340 --> 01:08:28,880
+minus 3، المفروض إنه يقع داخل ال range ما بين تلاتة
+
+898
+01:08:28,880 --> 01:08:33,000
+و تلاتين لتسعة و تلاتين في المية، طبعا لو بدي
+
+899
+01:08:33,000 --> 01:08:35,780
+أحولها letter per letter تلاتة و تلاتين من مئة
+
+900
+01:08:35,780 --> 01:08:40,240
+لتسعة و تلاتين من مئة letter per letter طبعا، إذا
+
+901
+01:08:40,240 --> 01:08:44,720
+أحكي ستة و تلاتين في المئة أو أحكي point ستة و
+
+902
+01:08:44,720 --> 01:08:48,620
+تلاتين من مئة letter per letter نفس المعنى، تمام؟
+
+903
+01:08:48,620 --> 01:08:52,620
+هذه طريقتين للتعبير عن قيمة ال hematocrit، إذن هيك
+
+904
+01:08:52,620 --> 01:08:56,940
+بنكون وصلنا لنهاية محاضرتنا اليوم، ان شاء الله
+
+905
+01:08:56,940 --> 01:09:01,640
+تكونوا استفدتوا واستمتعتوا في المحاضرة، واتمنى ان
+
+906
+01:09:01,640 --> 01:09:05,600
+يكون كل شيء واضح، ولو ما كان في شيء واضح أو عندكم
+
+907
+01:09:05,600 --> 01:09:10,140
+سؤال أو استفسار ما تترددوا تسألوني اما على group
+
+908
+01:09:10,140 --> 01:09:14,380
+ال whatsapp أو ان شاء الله من خلال الاجتماع ال Google meet
+
+909
+01:09:14,380 --> 01:09:17,880
+اللي احنا بنعقده اسبوعيا، يعطيكم
+
+910
+01:09:17,880 --> 01:09:20,240
+العافية، وموثقين بإذن الله
diff --git a/PL9fwy3NUQKwY9jl8xw-bWkVZkWcGGyspd/KXPW3iBAdP0_postprocess.srt b/PL9fwy3NUQKwY9jl8xw-bWkVZkWcGGyspd/KXPW3iBAdP0_postprocess.srt
new file mode 100644
index 0000000000000000000000000000000000000000..0f90c9919cb114a75ea0cafb35d1ab0bddcd41d1
--- /dev/null
+++ b/PL9fwy3NUQKwY9jl8xw-bWkVZkWcGGyspd/KXPW3iBAdP0_postprocess.srt
@@ -0,0 +1,3640 @@
+1
+00:00:05,900 --> 00:00:09,780
+السلام عليكم ورحمة الله وبركاته طالبات العزيزات ان
+
+2
+00:00:09,780 --> 00:00:13,380
+شاء الله اليوم رح نكون مع بعض بمحاضرة عملية بعنوان
+
+3
+00:00:13,380 --> 00:00:19,080
+micro-hematocrat طبعا micro-hematocrat ولكن قبل ما
+
+4
+00:00:19,080 --> 00:00:24,220
+نبدأ بمحاضرة micro-hematocrat خلونا نسترجع مع بعض
+
+5
+00:00:24,220 --> 00:00:28,060
+احنا ايش أخدنا بالمحاضرة النظرية السابقةتحدثنا عن
+
+6
+00:00:28,060 --> 00:00:32,280
+جهاز ال centrifuge حكينا عن المكونات جهاز ال
+
+7
+00:00:32,280 --> 00:00:36,140
+centrifuge حكينا أيضا على أنواع جهاز ال centrifuge
+
+8
+00:00:36,140 --> 00:00:40,200
+على مبدأ عمله و قوة الترد المركزي اللي من خلالها
+
+9
+00:00:40,200 --> 00:00:45,640
+بيتنفصل العينات و بحيث انه بتترسب الأجزاء الثقيلة
+
+10
+00:00:45,640 --> 00:00:49,520
+او المكونات الثقيلة في العينة في ال bottom of tube
+
+11
+00:00:49,520 --> 00:00:53,650
+و الأجزاء الخفيفة بتكونat surface او at that top
+
+12
+00:00:53,650 --> 00:01:00,030
+تمام؟ و ايضا تحدثنا عن التعليمات الهامة للاستخدام
+
+13
+00:01:00,030 --> 00:01:03,910
+instant refuse حكينا انه لازم انا اتبع ال
+
+14
+00:01:03,910 --> 00:01:07,290
+manufacturer recommendation اللي هي توصيات الجهة
+
+15
+00:01:07,290 --> 00:01:12,430
+المصنعة و انه انا ايضا لازم اخد بعين الاعتباربعض
+
+16
+00:01:12,430 --> 00:01:17,410
+التعليمات زي مثلا أن أحط الجهاز على بنش ثابت أسكّف
+
+17
+00:01:17,410 --> 00:01:21,490
+الجهاز قبل ما أنا أشغله ممنوع أوقف الجهاز إيقاف
+
+18
+00:01:21,490 --> 00:01:24,470
+مفاجئ أو أحاول أن أنا أبطئ سرعته بإيدي أثناء
+
+19
+00:01:24,470 --> 00:01:29,470
+إيقافه وغيرها من التعليمات اللي يجب مراعتها ويجب
+
+20
+00:01:29,470 --> 00:01:34,130
+اتباعها أثناء العمل أو تشغيل جهاز ال centrifuge
+
+21
+00:01:34,680 --> 00:01:40,120
+كتوظيف لمعرفتنا حول جهاز ال centrifuge رح ناخد
+
+22
+00:01:40,120 --> 00:01:44,220
+تطبيق عليه وهو فحص ال micro hematocrit خلونا
+
+23
+00:01:44,220 --> 00:01:46,980
+بالأول نشوف إيش يعني كلمة micro hematocrit
+
+24
+00:01:46,980 --> 00:01:54,280
+hematocrit لحالهاهي عبارة عن نسبة ال RBCs بالنسبة
+
+25
+00:01:54,280 --> 00:01:58,380
+لل whole blood أو ال volume اللي بتحتله ال RBCs،
+
+26
+00:01:58,380 --> 00:02:02,080
+الحجم اللي بتحتله ال RBCs بالنسبة للحجم الكل لعينة
+
+27
+00:02:02,080 --> 00:02:07,040
+الدم، تمام؟ النسبة المقاوية لل RBCs بالنسبة لل
+
+28
+00:02:07,040 --> 00:02:09,810
+whole blood أو عينة ال whole bloodو
+
+29
+00:02:09,810 --> 00:02:14,650
+microhematocrit هو يعني إضافة كلمة microhematocrit
+
+30
+00:02:14,650 --> 00:02:20,210
+ما هي إلا تعبير على إن أنا لما بشتغل الفحص هذا
+
+31
+00:02:20,210 --> 00:02:27,600
+باخد small amount of samplemicro sample من خلال ال
+
+32
+00:02:27,600 --> 00:02:32,560
+skin puncture طبعا من خلال ال skin puncture بشتغل
+
+33
+00:02:32,560 --> 00:02:35,140
+طبعا احنا حكينا micro sampling او ال skin puncture
+
+34
+00:02:35,140 --> 00:02:40,660
+نفس المعنى فإذا ال micro sampling انه انا باخد
+
+35
+00:02:40,660 --> 00:02:46,320
+عينة حجمها صغير في tube دقيق جدا رفيع جدا micro
+
+36
+00:02:46,320 --> 00:02:50,680
+tubeطبعا عشان هيك صار اسمه micro hematocrit طبعا
+
+37
+00:02:50,680 --> 00:02:55,000
+إذا إضافة micro فقط على التعبير عن حجم العينة وإنه
+
+38
+00:02:55,000 --> 00:02:58,340
+أنا باخد كمية قليلة من ال blood لحتى أشتغل هذا
+
+39
+00:02:58,340 --> 00:03:02,320
+الفحص micro hematocrit إله اسم آخر أو ال
+
+40
+00:03:02,320 --> 00:03:06,640
+hematocrit إله اسم آخرbacked cell volume of holy
+
+41
+00:03:06,640 --> 00:03:10,020
+blood إيش يعني backed cell volume؟ يعني أنا بدي
+
+42
+00:03:10,020 --> 00:03:15,720
+أخد حجم اللي بتحتله ال RBCs الخلايا أثناء بعد ما
+
+43
+00:03:15,720 --> 00:03:18,900
+يصيرلها packaging أو backed بعد ما يصير إيش صارلها
+
+44
+00:03:18,900 --> 00:03:22,180
+packaging يعني بعد ما صارلهاsedimentation أو
+
+45
+00:03:22,180 --> 00:03:25,480
+spinning أو centrifugation أو separation أنا يعني
+
+46
+00:03:25,480 --> 00:03:31,180
+جمعت ال RBCs لمّتها على بعضها يعني رسّبتها أو يعني
+
+47
+00:03:31,180 --> 00:03:36,940
+حصرتها في volume معين و بدي أشوف نسبة ال volume
+
+48
+00:03:36,940 --> 00:03:40,960
+هذا كام بتحتال نسبة ال RBCs بالنسبة لل holy blood
+
+49
+00:03:40,960 --> 00:03:44,880
+بالنسبة لحجم العينة كلهاإذن, hematocrite is
+
+50
+00:03:44,880 --> 00:03:49,900
+defined as the volume occupied by erythrocyte in a
+
+51
+00:03:49,900 --> 00:03:54,180
+given volume of blood and is usually expressed as
+
+52
+00:03:54,180 --> 00:03:56,780
+a percentage of the volume of the whole blood
+
+53
+00:03:56,780 --> 00:04:01,240
+sample. The hematocrite may also be referred to as
+
+54
+00:04:01,240 --> 00:04:05,660
+bagged cell volumeIran الهيماتوكره بيعرف على انه
+
+55
+00:04:05,660 --> 00:04:10,440
+ايش؟ ال volume occupied الحجم اللي بتحتله مين ال
+
+56
+00:04:10,440 --> 00:04:15,780
+RBCs فقط ال RBCs تمام؟ و طبعا بالنسبة لإيش؟
+
+57
+00:04:15,780 --> 00:04:19,100
+بالنسبة ل a given volume of blood بالنسبة للحجم
+
+58
+00:04:19,100 --> 00:04:24,700
+اللي هو ال total blood او ال whole bloodتمام؟ و ال
+
+59
+00:04:24,700 --> 00:04:28,560
+hematocrit بنسميه backs of volume و عرفنا إيش يعني
+
+60
+00:04:28,560 --> 00:04:32,780
+كلمة أو الترجمة الحرفية لbacks of volume فباختصار
+
+61
+00:04:32,780 --> 00:04:37,060
+شديد احنا اليوم راح نشتغل أو يعني راح نتعلم كتب و
+
+62
+00:04:37,060 --> 00:04:40,820
+بنشغل ال micro hematocrit من خلال أنه أنا أجيب
+
+63
+00:04:40,820 --> 00:04:45,000
+tube رفيعة جدا دقيقة جدا بسميها capillary tube
+
+64
+00:04:45,000 --> 00:04:49,420
+طبعا هاي ال capillary tube بتكون مفتوحة الطرفينو
+
+65
+00:04:49,420 --> 00:04:54,000
+انا ب .. ب .. ب .. من خلال skin puncture احداث
+
+66
+00:04:54,000 --> 00:04:57,380
+puncture في ال skin او من خلال ان انا اغمسها في
+
+67
+00:04:57,380 --> 00:05:01,780
+tube في blood فال .. الدم راح يطلع في هاي ال blood
+
+68
+00:05:01,780 --> 00:05:05,200
+او راح يملأ هاي ال capillary ال blood راح يطلع
+
+69
+00:05:05,200 --> 00:05:08,940
+يملأ هاي ال capillary بالخاصية الشعرية عشان هيك
+
+70
+00:05:08,940 --> 00:05:12,300
+بسميها capillary tube طبعا بال capillary action
+
+71
+00:05:12,300 --> 00:05:16,440
+بيملأ ال tube بالخاصية الشعرية ال capillary action
+
+72
+00:05:16,440 --> 00:05:20,250
+عشان هيك ال tube اسمها capillary tubeطبعا طيب بعد
+
+73
+00:05:20,250 --> 00:05:23,990
+ما أنا أملأ اليوتيوب لحتى تقريبا تلت أرباعها رح
+
+74
+00:05:23,990 --> 00:05:28,950
+أسكر طرف من أطرافها باستخدام clay اش يعني clay؟
+
+75
+00:05:28,950 --> 00:05:32,290
+معجونة أو طينة حرة المعجونة اللي بيلعبوا فيها
+
+76
+00:05:32,290 --> 00:05:34,930
+الأطفال هي اللي بتكون في ألعاب الأطفال هي اللي
+
+77
+00:05:34,930 --> 00:05:40,020
+باستخدمها طبعاطيب أصبح ليتيوب صارت مغلقة الطرفين
+
+78
+00:05:40,020 --> 00:05:43,980
+لو أنا حاطيتها في جهاز centrifuge خاص فيها بسميه
+
+79
+00:05:43,980 --> 00:05:48,860
+micro hematocrit centrifuge تمام؟ فراح يفصللياها
+
+80
+00:05:48,860 --> 00:05:53,340
+بحيث أنه الأجزاء الثقيلة راح تكون at the bottom
+
+81
+00:05:54,260 --> 00:05:57,440
+والأجزاء الخفيفة زي ال plasma هتكون at the top
+
+82
+00:05:57,440 --> 00:06:04,000
+فانا بحسب كام .. او يعني كام تحتل ال RBC بالنسبة
+
+83
+00:06:04,000 --> 00:06:07,760
+لل whole blood وبيطلع عندي قراقة ال hematocrit طب
+
+84
+00:06:07,760 --> 00:06:10,280
+انا لما احكي مثلا ال hematocrit تلاتين، ال
+
+85
+00:06:10,280 --> 00:06:15,910
+hematocrit اربعين، ستة و تلاتين، ستة و تلاتين اش36
+
+86
+00:06:15,910 --> 00:06:21,770
+في المية، اذا هو بنعبر عنه as a percentage، تمام؟
+
+87
+00:06:21,770 --> 00:06:25,990
+اذا بنعبر عنه as a percentage، نسبة مئوية، كام
+
+88
+00:06:25,990 --> 00:06:29,510
+تحتل نسبة ال .. ال RBCs بالنسبة لل holy blood؟ انا
+
+89
+00:06:29,510 --> 00:06:33,610
+بعتبر ان العينة كلها اللي انا جمعتها مية بالمية،
+
+90
+00:06:33,610 --> 00:06:38,650
+كام ال RBCs منها؟فرح نشوف كيف أنا ممكن أقيسها،
+
+91
+00:06:38,650 --> 00:06:43,070
+تمام؟ إذا هذه هي فكرة الفحص، إذا ال principle مبدأ
+
+92
+00:06:43,070 --> 00:06:48,790
+عمل الفحص أو فكرة الفحص أو يعني الافتراض أو نظرية
+
+93
+00:06:48,790 --> 00:06:53,350
+الفحص، مبدأ عمل الفحص هو إن أنا ال hematocrite is
+
+94
+00:06:53,350 --> 00:06:56,630
+usually determined by spinning a blood filled
+
+95
+00:06:56,630 --> 00:07:02,180
+capillary tube in a centrifugeبعمل determination،
+
+96
+00:07:02,180 --> 00:07:07,020
+قياس، معرفة للـ hematocrit من خلال ان انا اعمل
+
+97
+00:07:07,020 --> 00:07:11,700
+عملية spinning، centrifugation، separation،
+
+98
+00:07:11,700 --> 00:07:15,020
+sedimentation، لاحظين المصطلحات دي كلها نفس المعنى
+
+99
+00:07:15,020 --> 00:07:18,180
+لل blood في ال capillary blood في ال capillary،
+
+100
+00:07:18,180 --> 00:07:21,480
+capillary ممتلقة بال blood من خلال ال centrifuge
+
+101
+00:07:21,480 --> 00:07:26,180
+هاي capillary tube أنا عبقتها لتلت أربعها بال
+
+102
+00:07:26,180 --> 00:07:31,410
+bloodسكرتها بيه لكليه أصبحت مغلقة أحد الأطراف مغلق
+
+103
+00:07:31,410 --> 00:07:36,330
+و هذا before centrifuge after centrifuge راح يصير
+
+104
+00:07:36,330 --> 00:07:40,310
+فيه packaging لال RBC's ال RBC's كلها راح قاش
+
+105
+00:07:40,310 --> 00:07:44,890
+تتركز في منطقة واحدة اللي هي منطقة البطم يليها ال
+
+106
+00:07:44,890 --> 00:07:47,830
+buffy coat و يليها ال plasma هاي ال plasma هذه
+
+107
+00:07:47,830 --> 00:07:53,260
+كلها plasma تمام؟طبعا فأنا بدي أشوف كام تحتل ال
+
+108
+00:07:53,260 --> 00:07:57,600
+RBC بالنسبة ل ال whole blood لو افترضت ان هذه كلها
+
+109
+00:07:57,600 --> 00:08:02,600
+100% كام ال RBC من ال 100% هدول طبعا طيب العينة
+
+110
+00:08:02,600 --> 00:08:07,280
+تبعتي باخد اما .. اما venous blood يعني اما دم من
+
+111
+00:08:07,280 --> 00:08:12,350
+الوريد بصحبه و .. او من ال skin punctureطبعا إذا
+
+112
+00:08:12,350 --> 00:08:16,790
+أنا سحبت دم من الوريد وحطيته في edita tube في ال
+
+113
+00:08:16,790 --> 00:08:21,070
+tube اللي اللونها lavender ال cap تبعها لونه mauve
+
+114
+00:08:21,070 --> 00:08:24,950
+lavender طبعا هذا بيكون فيها edita anticoagulant
+
+115
+00:08:24,950 --> 00:08:31,720
+طبعا فإذا أنا حطيته فيهابس ممكن بعدها أعبي أو أملأ
+
+116
+00:08:31,720 --> 00:08:36,060
+capillary من خلاله أو ممكن مباشرة زي هنا في الصورة
+
+117
+00:08:36,060 --> 00:08:40,100
+من ال skin puncture أملأ ال capillary tube بس في
+
+118
+00:08:40,100 --> 00:08:44,040
+عندي شغلة ال capillary tube لو أنا هنا استعرضت
+
+119
+00:08:44,040 --> 00:08:48,900
+معاكم كيف شكل ال capillary tube و كيف أنواعها هاي
+
+120
+00:08:48,900 --> 00:08:52,640
+زي ما انتوا شايفين هنا في عندي capillary tube
+
+121
+00:08:55,110 --> 00:08:58,450
+هيها، هادى ال capillary tube هيك بتكون، طبعا بتكون
+
+122
+00:08:58,450 --> 00:09:03,510
+عندي علبة يعني فيها أكتر، يعني تقريبا ميت tube ال
+
+123
+00:09:03,510 --> 00:09:07,370
+capillary tube هاي بنات ده طبعا إلها color code
+
+124
+00:09:07,370 --> 00:09:12,670
+نفس فكرة الأنابيب وكل لون يعني إن هي في الداخل
+
+125
+00:09:12,670 --> 00:09:17,340
+ممسوحة بنوع معين من ال anticoagulantتمام؟ هاي اللي
+
+126
+00:09:17,340 --> 00:09:21,580
+لونها أحمر ممسوحة من الداخل بيه برين فهذه تستخدم
+
+127
+00:09:21,580 --> 00:09:26,620
+مباشرة ل ال blood اللي .. اللي من .. مجموع من skin
+
+128
+00:09:26,620 --> 00:09:31,000
+puncture لكن مثلا في هاي عندي نوع آخر من ال
+
+129
+00:09:31,000 --> 00:09:38,090
+capillary زي اللي لونهاblue طبعا، blue فهدي النوع
+
+130
+00:09:38,090 --> 00:09:41,730
+من الكابلري فاضي مافيش فيه إشي مش ممسوح بالداخل ب
+
+131
+00:09:41,730 --> 00:09:44,790
+anticoagulant بسميها bland احنا اتفقنا إذا مافيش
+
+132
+00:09:44,790 --> 00:09:48,270
+فيه إشي retube بسميه bland نفس الإشي الكابلري
+
+133
+00:09:48,270 --> 00:09:52,470
+لإنها مش .. من الداخل مافيش فيها anticoagulant فهي
+
+134
+00:09:52,470 --> 00:09:57,780
+blandcabulary طبعا هذه بجمع فيها أو بملقها من الدم
+
+135
+00:09:57,780 --> 00:10:01,780
+اللي من إيدي تتيوب يعني لو أنا جمعت blob من الوريد
+
+136
+00:10:01,780 --> 00:10:05,300
+و حطيته في إيدي تتيوب بعدين بدي أملق capillary و
+
+137
+00:10:05,300 --> 00:10:09,840
+أشتغل micro hematocrit بستخدم اللي بلان لكن لو بدي
+
+138
+00:10:09,840 --> 00:10:13,680
+أعمل skin puncture و مباشرة أملق capillary من ال
+
+139
+00:10:13,680 --> 00:10:16,660
+skin puncture بستخدم ال heparinase دي اللي لونها
+
+140
+00:10:16,660 --> 00:10:23,540
+أحمر طبعا مابنفعما بنفع أنا أجمع عينة blood في
+
+141
+00:10:23,540 --> 00:10:28,080
+إيديتا تيوب و أروح أستخدم الهيبرينized capillary
+
+142
+00:10:28,080 --> 00:10:33,800
+معاها يعني ما بنفع .. ما بنفع أنا .. ما بنفع أمرر
+
+143
+00:10:33,800 --> 00:10:37,580
+ال blood على نوعين anticoagulant بالأول إيديتا في
+
+144
+00:10:37,580 --> 00:10:42,460
+تيوب بعدين الهيبرين في ال capillaryممنوع ليش؟ لإن
+
+145
+00:10:42,460 --> 00:10:48,060
+هادي ال anticoagulant عبارة عن salts أملاح طبعا
+
+146
+00:10:48,060 --> 00:10:54,000
+طالما إن هي أملاح وجدت في العينة راح تأثر على
+
+147
+00:10:54,000 --> 00:10:57,980
+الضغط الأسموزي على ال osmolality للعينة والضغط
+
+148
+00:10:57,980 --> 00:11:03,080
+الأسموزي للخلايا فبما إنها أثرت على الضغط الأسموزي
+
+149
+00:11:03,080 --> 00:11:07,640
+للخلايا يعني اختلف شكل الخلايا طبعا اختلف شكل
+
+150
+00:11:07,640 --> 00:11:13,910
+الخلاياو طالما اختلف شكلها إذا راح تتأثر قراءة ال
+
+151
+00:11:13,910 --> 00:11:18,700
+hematocratو راح نشرح حد لاحقا خلال المحاضرات
+
+152
+00:11:18,700 --> 00:11:23,320
+الجاية طبعا ال .. ال .. ال specimen لازم انا
+
+153
+00:11:23,320 --> 00:11:26,620
+العينة تبعتي سواء كان يعني specimen هي نفسها
+
+154
+00:11:26,620 --> 00:11:30,900
+معناها sample نفس الاشي عينة طبعا العينة تبعتي
+
+155
+00:11:30,900 --> 00:11:34,200
+لازم انا افصلها باستخدام ال centrifuge should be
+
+156
+00:11:34,200 --> 00:11:37,820
+centrifuged within six hours of collection يعني
+
+157
+00:11:37,820 --> 00:11:43,940
+خلال ست ساعاتمن جمع العينة لازم انا اكون اعمل
+
+158
+00:11:43,940 --> 00:11:50,140
+centrifugation لازم اكون عملت centrifugation ليش؟
+
+159
+00:11:50,140 --> 00:11:55,260
+لأنه اذا انا بدي اتأخر اكتر من هيك ممكن تتأثر يعني
+
+160
+00:11:55,260 --> 00:12:00,540
+تتغيرمكونات العينة، في خلايا ممكن تتكسر، تتحلل،
+
+161
+00:12:00,540 --> 00:12:03,560
+يصير في عندي فاقد في الخلايا، وأنا أصلا قراءتي هي
+
+162
+00:12:03,560 --> 00:12:06,560
+عبارة عن نسبة خلايا، حجم اللي بتحتل الخلايا
+
+163
+00:12:06,560 --> 00:12:10,160
+بالنسبة لل holy blood، فإذا صار في عندي فاقد، إذن
+
+164
+00:12:10,160 --> 00:12:14,680
+هيكون في عندي يعني فاقد أيضا في القراءة، في عندي
+
+165
+00:12:14,680 --> 00:12:21,060
+نسبة قراءة ناقصة، يعني decreased، طبعا، في قراءة
+
+166
+00:12:21,060 --> 00:12:25,920
+ال hematocritHemolyse sample can't be used for
+
+167
+00:12:25,920 --> 00:12:31,300
+testing عينة تمت .. يعني صار فيها تكسر في الخلايا
+
+168
+00:12:31,300 --> 00:12:36,260
+أيضا مش proper مش جيدة لحتى أنا أشتغل فيها
+
+169
+00:12:36,260 --> 00:12:41,500
+hematocrit why؟ لإن ال hemolyse sample بيصير فيه
+
+170
+00:12:41,500 --> 00:12:45,240
+فقد عندي في الخلايا اتكسرت خلايا ال RBCs طب أنا
+
+171
+00:12:45,240 --> 00:12:50,140
+أصلا عم بحسب حجم ال RBCs حجم ال erythrocyte
+
+172
+00:12:50,140 --> 00:12:55,140
+بالنسبة لل holy bloodطالما صار في عندي فقد في هذه
+
+173
+00:12:55,140 --> 00:12:59,480
+الخلايا اتكسرت معناها ايضا القراءة تبعتي راح يصير
+
+174
+00:12:59,480 --> 00:13:03,560
+فيها ايش؟ راح تكون .. تصير minus، في نقص فيها،
+
+175
+00:13:03,560 --> 00:13:07,440
+يعني في decrease في القراءة وهي مشكلة، ال decrease
+
+176
+00:13:07,440 --> 00:13:11,360
+مش .. مش جاية من حالة المريض، لأ هي جاية من ال
+
+177
+00:13:11,360 --> 00:13:15,040
+technical error تبعي، انه انا اتأخرت على العينة،
+
+178
+00:13:15,040 --> 00:13:17,700
+اتعاملت مع العينة بشكل خاطئ فصار في تكسر في
+
+179
+00:13:17,700 --> 00:13:25,010
+الخلايا وها كدهطيب إيش بلزمني أدوات ومواد لحتى
+
+180
+00:13:25,010 --> 00:13:28,610
+أشتغل ال hematocrit؟ أول إشي بلزمني capillary tube
+
+181
+00:13:28,610 --> 00:13:33,050
+إما تكون heparinized يعني red tip احنا حكينا إن
+
+182
+00:13:33,050 --> 00:13:39,210
+اللي معلمة بي ال red بالخط الأحمر من طرفها هدي
+
+183
+00:13:39,210 --> 00:13:44,530
+فيها heparin أما إذا كانت bland فهي blue يعني
+
+184
+00:13:44,530 --> 00:13:47,830
+بتكون blue tip يعني إذا كانت فاضية فهي blue أربطها
+
+185
+00:13:47,830 --> 00:13:56,780
+بي blandP Plain و P Blue Plain فلونها blue احفظوها
+
+186
+00:13:56,780 --> 00:14:01,500
+هك تمام؟ ومحتاجة clay اللي حكينا عنه المعجونة او
+
+187
+00:14:01,500 --> 00:14:05,420
+الطينة و ال micro hematocrit centrifuge محتاجة
+
+188
+00:14:05,420 --> 00:14:09,620
+readerتدريج خاص لحتى أقرأ عليه لحتى أعتبر أن
+
+189
+00:14:09,620 --> 00:14:13,940
+العينة كلها 100 و أشوف نسبة ال RBC منها هذا بيتم
+
+190
+00:14:13,940 --> 00:14:20,120
+من خلال تدريج خاص محتاجة جوز شاش طبعا او يعني قطعة
+
+191
+00:14:20,120 --> 00:14:26,500
+كماشية قطنية لحتى انا يعني انظف ال tube اذا تم
+
+192
+00:14:26,500 --> 00:14:30,160
+غمرها ب tube blood اذا انا انظف ال capillary من
+
+193
+00:14:30,160 --> 00:14:34,100
+برا اذا تم غمرها ب tube blood و اتبهدلت من برا انا
+
+194
+00:14:34,100 --> 00:14:38,380
+بدي انظفهاطبعا باستخدام ايش يا بنات؟ Goose شاش
+
+195
+00:14:38,380 --> 00:14:44,260
+يعني اللي هو زي شاش لجروح، تمام؟ طيب هاي زي ما
+
+196
+00:14:44,260 --> 00:14:48,060
+انتوا شايفين في الصورة ال capillary أنواع و كل نوع
+
+197
+00:14:48,060 --> 00:14:51,740
+له color code زي فكرة الأنابيب يا بنات،يلها color
+
+198
+00:14:51,740 --> 00:14:55,920
+code كل نوع معلم بلون زي لون الغطاية بيكون ايضا
+
+199
+00:14:55,920 --> 00:14:59,830
+معلمة لل capillary نفسها في هذا اللونوكل لون يعني
+
+200
+00:14:59,830 --> 00:15:03,930
+محتوها مختلف احنا بس هلاقى حاليا المطلوب مننا انه
+
+201
+00:15:03,930 --> 00:15:09,070
+نعرف ان ال red فيها heparin و لبلان blue طبعا هذا
+
+202
+00:15:09,070 --> 00:15:12,930
+اللي بدياكم تعرفو حاليا و بدياكم تعرفوا اذا العينة
+
+203
+00:15:12,930 --> 00:15:16,350
+جاية من skin puncture معناه انا بدي استخدم ال
+
+204
+00:15:16,350 --> 00:15:21,350
+heparinized capillary tubeوإذا العينة جاية من vain
+
+205
+00:15:21,350 --> 00:15:25,390
+من وريد و أنا فضيتها في إيدي تتيوب و بدى أملأ
+
+206
+00:15:25,390 --> 00:15:28,970
+capillary بستخدم bland لإن العينة already صارت
+
+207
+00:15:28,970 --> 00:15:32,410
+anticoagulated already مضعف عليها anticoagulant
+
+208
+00:15:32,410 --> 00:15:35,810
+اللي هو الإيدي تعالى فمش محتاجة أنا أصلا أستخدم
+
+209
+00:15:35,810 --> 00:15:41,120
+capillary أيضا فيها anticoagulant تمام؟طيب، إيش هي
+
+210
+00:15:41,120 --> 00:15:45,840
+ال procedure التجري ده اللي أنا بستخدمها أو ب ..
+
+211
+00:15:45,840 --> 00:15:50,620
+بجري من خلالها ال hematocrit؟ أول إشي بيحكيلك في
+
+212
+00:15:50,620 --> 00:15:54,380
+ال two capillary tube approximately three quarters
+
+213
+00:15:54,380 --> 00:15:59,130
+full with blood anticoagulatedeither with edita or
+
+214
+00:15:59,130 --> 00:16:03,710
+with hiberin يعني بدي أملأ two capillary tube
+
+215
+00:16:03,710 --> 00:16:08,410
+تقريبا لتلت أرباعهم بال blood اللي anticoagulated
+
+216
+00:16:08,410 --> 00:16:12,190
+اللي مضعف عليه anticoagulant إما edita أو إذا كان
+
+217
+00:16:12,190 --> 00:16:15,290
+أنا بستخدم حكاية skin puncture على طول باخد ال
+
+218
+00:16:15,290 --> 00:16:19,930
+hiberin طيب ال hiberinized capillary tubeهلأ ال
+
+219
+00:16:19,930 --> 00:16:23,910
+blood for hiperionized capillary tube حكينا أنه
+
+220
+00:16:23,910 --> 00:16:26,950
+إيش من إيش أنا باخده من ال puncture من ال skin
+
+221
+00:16:26,950 --> 00:16:31,650
+puncture لكن اللي من ال edita بفضيه في plain
+
+222
+00:16:31,650 --> 00:16:36,390
+capillary tube هذه الجزئية أنا كررتها كذا مرة لحتى
+
+223
+00:16:36,390 --> 00:16:42,010
+أنه تفهموها لأنها مهمة يا بناتطيب بعد ما أنا أملأ
+
+224
+00:16:42,010 --> 00:16:45,370
+ال tube بتلات .. بتلات أربعها طبعا حكينا إن ال
+
+225
+00:16:45,370 --> 00:16:49,290
+blood بيملأها بالخاصية الشعرية capillary action
+
+226
+00:16:49,290 --> 00:16:53,450
+seal the end of the tube with the non-colouring
+
+227
+00:16:53,450 --> 00:17:00,510
+ring with the non-absorbent clay هلأ يعني إنه من
+
+228
+00:17:00,510 --> 00:17:07,750
+الجهة المش ملونة مثلا سكري ال capillary بالclay
+
+229
+00:17:07,750 --> 00:17:16,040
+يعنيفوتي .. يعني هيك ..فوتيها في داخل ال container
+
+230
+00:17:16,040 --> 00:17:20,560
+او يعني bottle او cup موجود فيه ال clay فهتلاحظى
+
+231
+00:17:20,560 --> 00:17:24,820
+انه ال clay هيدخل جوا ال .. ال capillary و يسكر
+
+232
+00:17:24,820 --> 00:17:29,940
+الفتحة احد الطرفين balance the tube in the
+
+233
+00:17:29,940 --> 00:17:32,600
+centrifuge with the clay طبعا انا بستخدم two
+
+234
+00:17:32,600 --> 00:17:36,520
+capillary للعينة الواحدة why؟ او ليش؟ عشان انا
+
+235
+00:17:36,520 --> 00:17:40,550
+اعمل ال .. ال .. ال tube بدها balance تمام؟تاني
+
+236
+00:17:40,550 --> 00:17:44,530
+إشي لإنه ب .. بجيب القراءة على ال two capillaries
+
+237
+00:17:44,530 --> 00:17:49,670
+tube و بعدين ب .. بجيب ال average لإلهم و طالما
+
+238
+00:17:49,670 --> 00:17:52,170
+أنا جبت ال average لقراقتين معناها أنا more
+
+239
+00:17:52,170 --> 00:17:57,280
+accurate and preciseبحط الأنابيب في ال centrifuge
+
+240
+00:17:57,280 --> 00:18:03,100
+مقابل بعضها البعض و بعد هيك بشغل جهاز ال
+
+241
+00:18:03,100 --> 00:18:07,040
+centrifuge لمدة خمس دقائق على سرعة ما بين العشر
+
+242
+00:18:07,040 --> 00:18:12,060
+ألف إلى خمستاشر ألف round per minuteuse the brake
+
+243
+00:18:12,060 --> 00:18:14,980
+to stop the centrifuge ممنوع أنا أستخدم ال brake
+
+244
+00:18:14,980 --> 00:18:19,060
+لحتى أوقف ال centrifuge لإنه إذا حكينا إحنا قبل
+
+245
+00:18:19,060 --> 00:18:23,080
+هيك إنه انا إذا عملت إيقاط مفاجئ لل centrifuge
+
+246
+00:18:23,080 --> 00:18:27,920
+معناها ال sediment اللي اترسب راح يندفع باتجاه
+
+247
+00:18:27,920 --> 00:18:30,780
+الأعلى فبالتالي راح يصير في mixing للعينة أو
+
+248
+00:18:30,780 --> 00:18:34,520
+انفصال لل sediment وهذا راح يعطيني error خطأ في
+
+249
+00:18:34,520 --> 00:18:40,050
+القراءةطبعا بعد ما يتوقف الجهاز لأ يعني بعد ما
+
+250
+00:18:40,050 --> 00:18:43,670
+ينتهي يقول خمسة minute بيرن جرس يعني عشان تنبيه
+
+251
+00:18:43,670 --> 00:18:49,870
+أنه انتهت المدة وبيبدأ الجهاز يبطئ سرعته مع نفسه
+
+252
+00:18:49,870 --> 00:18:56,550
+طبعا إلى أن يقف يعني to stopبعد ما الجهاز يعني ت
+
+253
+00:18:56,550 --> 00:19:03,070
+stop يقف ب .. انا بفتح ال .. ال cover تبعه و باخد
+
+254
+00:19:03,070 --> 00:19:07,770
+القنابيب و باستخدام تدريج خاص ب .. بعمل
+
+255
+00:19:07,770 --> 00:19:10,430
+determination لل hematocrit، بعرف ال hematocrit
+
+256
+00:19:13,130 --> 00:19:16,330
+كيف هذا .. فكرة ال .. ال reader تبعي؟ زي ما انتوا
+
+257
+00:19:16,330 --> 00:19:20,330
+شايفين هو عبارة عن تدريج بيبدأ بالصفر و بينتهي
+
+258
+00:19:20,330 --> 00:19:26,090
+بالمية، طبعا؟ هذا التدريج بتشوفوه زي عم بيتسع كل
+
+259
+00:19:26,090 --> 00:19:30,330
+ما اتجهنا بهذا الاتجاه، طبعا؟ ضيق هنا و بيتسع هنا
+
+260
+00:19:30,330 --> 00:19:34,830
+و نفس الفكرة انه ال .. ال .. بيدعته صفر و نهايته
+
+261
+00:19:34,830 --> 00:19:39,080
+ميةطب ليش .. ليش عم بالتساع؟ لحتى يناسب جميع
+
+262
+00:19:39,080 --> 00:19:42,280
+الأطوال و جميع محتويات العينة يعني انا لو .. لو
+
+263
+00:19:42,280 --> 00:19:46,300
+عينة طويلة أو تيوب طويلة أو عينة قصيرة، تيوب
+
+264
+00:19:46,300 --> 00:19:49,440
+مالقتها كتير، تيوب ما .. ما .. ما عبيتها كتير
+
+265
+00:19:49,440 --> 00:19:53,640
+فبالتالي انه في النهاية يكون التدريج مناسب لإلها،
+
+266
+00:19:53,640 --> 00:19:56,040
+لا تيوب طويلة و الله بروح على الجهة الطويلة من
+
+267
+00:19:56,040 --> 00:20:01,540
+التدريج، تيوب قصيرة أو يعني تيوب محتواها قليل،
+
+268
+00:20:01,540 --> 00:20:06,260
+بروح على الجهة القصيرة من التدريجبروح بطابق يعني
+
+269
+00:20:06,260 --> 00:20:09,140
+زي ما انتوا شايفين بداية ال blood لازم تكون عند خط
+
+270
+00:20:09,140 --> 00:20:13,720
+الصفر و نهاية ال plasma لازم تكون متابقة لخط
+
+271
+00:20:13,720 --> 00:20:18,340
+المياهبعدين بشوف ال RBCs و بقرأ من تحت ال buffer
+
+272
+00:20:18,340 --> 00:20:23,880
+code بشوف هي مقابل لأي قراءة و بعرف كام القراءة ال
+
+273
+00:20:23,880 --> 00:20:27,820
+RBCs يعني كام نسبة ال RBCs بالنسبة لل holy blood
+
+274
+00:20:27,820 --> 00:20:32,920
+تمام؟ هي فكرة استخدام التدريج عندنا أنواع و ..
+
+275
+00:20:32,920 --> 00:20:35,860
+يعني أشكال مختلفة من التدريج راح ان شاء الله بس
+
+276
+00:20:35,860 --> 00:20:38,700
+نرجع المختبرات راح أعلمكم عليها و كيف تستخدموها
+
+277
+00:20:38,700 --> 00:20:44,640
+تمام؟بحكيلي وانت بتقرا ايه؟ don't include the
+
+278
+00:20:44,640 --> 00:20:48,020
+buffy coat, leukocyte and the platelet why؟ احنا
+
+279
+00:20:48,020 --> 00:20:51,260
+بنقرا micro hematocrit ال volume occupied by
+
+280
+00:20:51,260 --> 00:20:57,520
+erythrocyte by erythrocyte طبعا؟ الحجم اللي بتحتله
+
+281
+00:20:57,520 --> 00:21:03,330
+ال RBCs فقط لغانفلذلك انا ما بدخل .. ما .. ال ..
+
+282
+00:21:03,330 --> 00:21:06,610
+ال .. يعني مابحسب ال .. ال buffy code في القراءة
+
+283
+00:21:06,610 --> 00:21:10,210
+don't include the buffy code in the reading تمام؟
+
+284
+00:21:10,210 --> 00:21:13,970
+ممنوع تدرجي او يعني تحسبي ال buffy code داخل
+
+285
+00:21:13,970 --> 00:21:18,170
+القراءة طبعتك انا بقرأ من تحت ال buffy code تمام؟
+
+286
+00:21:18,170 --> 00:21:21,490
+اللازم ال tubes .. ال tubes تبعتي ال two tubes
+
+287
+00:21:21,490 --> 00:21:26,160
+يكون على الأقليعني او يعني على .. على اكتر الفرق
+
+288
+00:21:26,160 --> 00:21:29,680
+ما بينهم اتنين في المية، ايش يعني؟ يعني ممكن يكون
+
+289
+00:21:29,680 --> 00:21:33,740
+الفرق ما بينهم نصف في المية، واحد في المية، اتنين
+
+290
+00:21:33,740 --> 00:21:37,180
+في المية، plus minus طبعا يا بنات، اكتر من هيك
+
+291
+00:21:37,180 --> 00:21:40,320
+ممنوع، يعني plus minus تلاتة لأ، plus minus اتنين
+
+292
+00:21:40,320 --> 00:21:44,100
+و نصف لأ، معناه في variation كتير في النتائج هيك،
+
+293
+00:21:44,100 --> 00:21:46,140
+انا ممنوع .. مش لازم يكون في عندي variation، لازم
+
+294
+00:21:46,140 --> 00:21:50,830
+يكون في تقارب في النتائجتمام، إيش يعني الكلام هذا؟
+
+295
+00:21:50,830 --> 00:21:54,510
+لنفرض إنه في عندي tube اللي hematocrite فيها طلع
+
+296
+00:21:54,510 --> 00:22:00,910
+36%، تمام؟ معناه ال tube التانية كام لازم يطلع؟
+
+297
+00:22:00,910 --> 00:22:06,330
+ممكن يطلع 36 زيها بالظبط، ممكن 36.5 أو 35.5 يعني
+
+298
+00:22:06,330 --> 00:22:11,250
+plus minus نص، تمام؟ممكن يكون خمسة و تلاتين أو
+
+299
+00:22:11,250 --> 00:22:14,890
+سبعة و تلاتين plus minus واحد هيك تمام؟ ممكن
+
+300
+00:22:14,890 --> 00:22:19,830
+يكونوا اربعة و تلاتين او تمانية و تلاتين هيك plus
+
+301
+00:22:19,830 --> 00:22:25,170
+minus اتنين بس نهائيا .. نهائيا ما بقبل نتيجة اذا
+
+302
+00:22:25,170 --> 00:22:29,130
+كانت واحدة فيهم قيمتها ستة و تلاتين مثلا و القيمة
+
+303
+00:22:29,130 --> 00:22:31,610
+التانية تلاتة و تلاتين يعني في فرق ما بينهم تلاتة
+
+304
+00:22:31,610 --> 00:22:34,910
+في المية او اذا كانت ستة و تلاتين و التانية تسعة و
+
+305
+00:22:34,910 --> 00:22:38,030
+تلاتين في فرق ما بينهم تلاتة في المية غلطلازم
+
+306
+00:22:38,030 --> 00:22:40,910
+within اتنين في المية يكون الفارق ما بينهم علشان
+
+307
+00:22:40,910 --> 00:22:45,790
+احكي و الله انه في تقارب في نتيجة تبعتي تمام؟ باخد
+
+308
+00:22:45,790 --> 00:22:49,530
+القيمتين بقرأ ال .. ال .. ال two tubes و بجيب ال
+
+309
+00:22:49,530 --> 00:22:52,870
+average تبعهم علشان اكون more accurate and precise
+
+310
+00:22:54,880 --> 00:22:59,440
+طيب خلّينا بس نشوف كيف شكل ال centrifuge اللي احنا
+
+311
+00:22:59,440 --> 00:23:02,160
+.. احنا حكينا ان في عندي micro hematocrit
+
+312
+00:23:02,160 --> 00:23:05,840
+centrifuge هاي شكل ال centrifuge طبعا هذا typical
+
+313
+00:23:05,840 --> 00:23:09,380
+موجود عندنا بالمختبرات زيه، typically، يعني أخوه،
+
+314
+00:23:09,380 --> 00:23:13,710
+نفس الألوان، نفس الشكلهذا ال cover الخارجي و في
+
+315
+00:23:13,710 --> 00:23:18,710
+cover داخلي تمام و هذا تدريج timer هو already set
+
+316
+00:23:18,710 --> 00:23:22,330
+على سرعة معينة يعني انا مابحدد سرعته هذا الجرس
+
+317
+00:23:22,330 --> 00:23:26,590
+الإنذار انه يعني توقف الجهاز و هذا ال break تمام
+
+318
+00:23:26,590 --> 00:23:30,730
+لو جيت انا فتحت ال cover الخارجي و الداخلي كيف رح
+
+319
+00:23:30,730 --> 00:23:35,860
+يكون شكل ال .. ال .. ال hematocritزي ما انتوا
+
+320
+00:23:35,860 --> 00:23:41,320
+شايفين بهذا الشكل بيكون شكل ال .. ال .. ال .. ال
+
+321
+00:23:41,320 --> 00:23:45,240
+micro hematocrit centrifuge من الداخل ال .. هذه ال
+
+322
+00:23:45,240 --> 00:23:49,680
+head وهي فيها buckets فيها فرزات تمام؟ هذا الفرز
+
+323
+00:23:49,680 --> 00:23:52,980
+مخصص لل capillary هذا عشان هيك أنا بسميه
+
+324
+00:23:52,980 --> 00:23:56,520
+centrifuge خاص بال hematocrit التيوب لإن هو
+
+325
+00:23:56,520 --> 00:23:59,940
+مابيستوعبش تيوبات كبيرة هو بيستوعب فقط ال .. ال ..
+
+326
+00:23:59,940 --> 00:24:06,200
+ال micro hematocrit تمام؟ طيبهدا بس ال .. ال ..
+
+327
+00:24:06,200 --> 00:24:10,280
+الشكل ال micro centrifuge أو ال micro hematocrite
+
+328
+00:24:10,280 --> 00:24:13,900
+ال centrifuge من الداخل هاي كمان صورة أخرى لإله
+
+329
+00:24:13,900 --> 00:24:18,580
+شايفين؟ هاد .. هاد ال central shaft هادي ال head
+
+330
+00:24:19,040 --> 00:24:24,000
+وفيها buckets طبعا؟ انتوا شوفتوا ال buckets في ال
+
+331
+00:24:24,000 --> 00:24:28,940
+centrifuges التانية عبارة عن مكان زي وعاء بيشبه
+
+332
+00:24:28,940 --> 00:24:35,820
+شكل ال tube طبعا؟ هذا أيضا فرز او زي مكان مخصص
+
+333
+00:24:35,820 --> 00:24:39,780
+بيشبه شكل ال capillary بيشبه شكل ال capillary tube
+
+334
+00:24:41,240 --> 00:24:46,060
+لذلك هو مخصص لـ micro-hematocrit هذا الجهاز للـ
+
+335
+00:24:46,060 --> 00:24:48,600
+centrifuge هذه شايفين هي الغطاية الداخلية وهي
+
+336
+00:24:48,600 --> 00:24:52,140
+الغطاية الخارجية طبعا يا بنات لما بحط لي tubes
+
+337
+00:24:52,140 --> 00:24:57,760
+لازم أراعي ال balance كل tube مقابل التانية كل
+
+338
+00:24:57,760 --> 00:25:02,940
+tube مقابل التانيةتمام؟ طيب و في شغلة لازم
+
+339
+00:25:02,940 --> 00:25:08,160
+أنتبهلها أنه لما أسكر ال tube بال clay لازم
+
+340
+00:25:08,160 --> 00:25:12,840
+المنطقة المغلقة وين تكون؟ تكون على الحواف تمام؟
+
+341
+00:25:12,840 --> 00:25:16,580
+تكون هنا المنطقة المغلقة أو هذه المنطقة المفتوحة
+
+342
+00:25:16,910 --> 00:25:21,910
+ليش بقى انتبه لهاي الشغلة؟ لإنه هذا جهاز طارد
+
+343
+00:25:21,910 --> 00:25:24,830
+مركزي، الأجزاء .. الأجزاء الثقيلة وين راح تروح؟
+
+344
+00:25:24,830 --> 00:25:27,890
+راح تحدد تروح بعيد عن ال central shaft، بعيد عن
+
+345
+00:25:27,890 --> 00:25:32,210
+المركز، وين راح تقى تمطردها؟ بهذا الاتجاه، يعني
+
+346
+00:25:32,210 --> 00:25:36,380
+هنا ال RBCs راح تتجمعاتخيلي لو المكان هذا مفتوح مش
+
+347
+00:25:36,380 --> 00:25:40,080
+مسكر بالـ Clay، إيش يعني؟ يعني راح يصير .. يصير
+
+348
+00:25:40,080 --> 00:25:42,960
+طردها خالص، يعني إحنا طردناها و زيادة و كت ..
+
+349
+00:25:42,960 --> 00:25:49,160
+عملنا و كترنا، فبصير إيش؟ طردنا ال .. ال .. ال ..
+
+350
+00:25:49,160 --> 00:25:54,040
+ال RBCs خارج التيوب و ليس at the bottom of التيوب
+
+351
+00:25:54,540 --> 00:25:58,420
+طبعا فعشان هيك لازم المنطقة المغلقة تكون في هذا
+
+352
+00:25:58,420 --> 00:26:02,920
+الاتجاه عشان لما تيجي ال RBCs يتم طردها مركزيا او
+
+353
+00:26:02,920 --> 00:26:06,120
+يعني تترثب او يصير لها sedimentation separation في
+
+354
+00:26:06,120 --> 00:26:09,620
+at the bottom of the tube يكون فيها عائق على الأقل
+
+355
+00:26:09,620 --> 00:26:15,380
+يمنعها من انها هي يصير لها loss فقدان طبعا اتخيلي
+
+356
+00:26:15,380 --> 00:26:19,150
+لو انا عكستو في .. بيصير في طارد مركزي إيش يعني؟
+
+357
+00:26:19,150 --> 00:26:22,270
+إن أنا مساكت الأتيوب و كباتها، هاي معناها، و كأنك
+
+358
+00:26:22,270 --> 00:26:27,010
+يا أبو زاد ما غزيت، تمام؟ أنا بكون كبات إيدي ..
+
+359
+00:26:27,010 --> 00:26:34,860
+أنا بيبي بإيدياطيب ال .. ال .. ال cover الداخلي
+
+360
+00:26:34,860 --> 00:26:38,740
+هذا بيغلق .. بيتم إغلاقه من خلال ترس يا بنات سم
+
+361
+00:26:38,740 --> 00:26:44,440
+يعني بيحبك على ال capillary tube بعدين بسكر ال
+
+362
+00:26:44,440 --> 00:26:48,160
+cover الخارجي عشان هيك لازم أتأكد أن كل tube
+
+363
+00:26:48,160 --> 00:26:51,280
+موجودة داخل الفرز المخصص لإلها مش طالع على بره أو
+
+364
+00:26:51,280 --> 00:26:55,520
+برزه لإنه إذا كانت مش موجودة بال .. بالفرز يعني
+
+365
+00:26:55,520 --> 00:27:00,310
+تماما موجودة داخل الفرز المخصص لإلهاهاي ال .. ال
+
+366
+00:27:00,310 --> 00:27:05,050
+.. الغطاية لما أجي أسكرها و تحبك راح تطحم ال tube
+
+367
+00:27:05,050 --> 00:27:09,530
+ال cabillary راح تكسرها، تفتفتها، طبعا؟ و هيك أنا
+
+368
+00:27:09,530 --> 00:27:15,630
+بكون فقدت العينة تبعتيفإذا احذروا ان تفقدوا العينة
+
+369
+00:27:15,630 --> 00:27:20,430
+تبعتكم اتأكدي ان ال .. ال tube جالسة بالمكان
+
+370
+00:27:20,430 --> 00:27:25,350
+المخصص لإلها بالفرز المخصص لإلها ومنطقة المغلقة
+
+371
+00:27:25,350 --> 00:27:29,990
+بال clay هي اللي موجودة outside بعيدة عن المركز و
+
+372
+00:27:29,990 --> 00:27:34,590
+المنطقة المفتوحة هي اللي أقرب ما يكون لمركزها تمام
+
+373
+00:27:36,940 --> 00:27:41,140
+طيب بعد ما أنا خلصت وحاكينا ب .. ب .. بحطها على
+
+374
+00:27:41,140 --> 00:27:46,400
+التدريج الخاص فيها و بحط بداية ال blood عند خط ال
+
+375
+00:27:46,400 --> 00:27:50,960
+zero و نهاية ال plasma عند خط المية بقرا ال .. ال
+
+376
+00:27:50,960 --> 00:27:56,860
+.. نسبة ال RBCs بالنسبة يعني لل whole blood و من
+
+377
+00:27:56,860 --> 00:27:59,680
+تحت ال buffy quote بقرا يعني أنا بس بدي نسبة ال ..
+
+378
+00:27:59,680 --> 00:28:04,430
+ال erythrocyte فقط لا غيرطيب يا بنات هذه ال
+
+379
+00:28:04,430 --> 00:28:08,550
+reference ranges يعني ال normal range حكينا إنه ال
+
+380
+00:28:08,550 --> 00:28:14,070
+normal value أنا بعبّر عنها ب range و ليس قيمة
+
+381
+00:28:14,070 --> 00:28:18,890
+واحدة ل newborn إيش قيمة الهيمتكرة الطبيعية عندهم
+
+382
+00:28:18,890 --> 00:28:26,530
+من 53% ل 65%؟ طب ليش أنا بحكي 53% ل 65%؟ هي عالية
+
+383
+00:28:26,530 --> 00:28:29,970
+أنتم لاحظيني أنا مقارنة بالقيم التانية هي أعلى إشي
+
+384
+00:28:30,550 --> 00:28:34,990
+حكينا لإنه ال newborn الأطفال حديثي الولادة
+
+385
+00:28:34,990 --> 00:28:39,030
+بيولدوا عندهم نسبة ال hemoglobin عالية كتير و ال
+
+386
+00:28:39,030 --> 00:28:42,310
+RBCs قيمتها بتكون عالية كتير يعني عندهم خلايا
+
+387
+00:28:42,310 --> 00:28:46,750
+عالية عندهم خلايا كتيرة ليش؟ لإنه أول حاجة هم في
+
+388
+00:28:46,750 --> 00:28:50,810
+حالة growth burst يعني انفجار في النمو عندهم يعني
+
+389
+00:28:50,810 --> 00:28:56,030
+هم في حالة نمو هائل تمام؟ و فمحتاجين oxygen
+
+390
+00:28:56,030 --> 00:29:03,850
+delivery يعني محتاجينRBCs كتيرة و أيضا عندهم مشكلة
+
+391
+00:29:03,850 --> 00:29:07,470
+ان ال homoglobin تبعهم ال affinity تبعته الجاذبية
+
+392
+00:29:07,470 --> 00:29:10,910
+تبعته للأكسوجين عالية يعني لما بيروح يوصل ال
+
+393
+00:29:10,910 --> 00:29:15,590
+oxygen لل tissue تمام بيكون مش هاين عليه يترك ال
+
+394
+00:29:15,590 --> 00:29:21,140
+oxygen تمام بصعوبة بيترك ال oxygenفبالتالي ربنا
+
+395
+00:29:21,140 --> 00:29:25,640
+عوضهم بزيادة RBCs وزيادة Hemoglobin طبعا إذا في
+
+396
+00:29:25,640 --> 00:29:28,680
+سبب بين أول حاجة أن ال Hemoglobin تبعهم إله
+
+397
+00:29:28,680 --> 00:29:32,420
+affinity عالية للأكسوجين تاني إشي لإنهم في حالة
+
+398
+00:29:32,420 --> 00:29:36,000
+growth burst طبعا خليني أورجيكم إيه كيف .. إيش
+
+399
+00:29:36,000 --> 00:29:42,180
+يعني كيف تنكتب كلمة growth burst طبعا growth burst
+
+400
+00:29:43,530 --> 00:29:47,410
+هدى الحالة اللى توصف وضع النمو عند ال .. ال
+
+401
+00:29:47,410 --> 00:29:53,030
+newborn تمام؟ طيب ال infant او ال child يعني لبس
+
+402
+00:29:53,030 --> 00:29:56,890
+يكبر شوية بتبدأ ايش ال homoglobin تبعهم اتقل ال
+
+403
+00:29:56,890 --> 00:30:01,710
+affinity تبعته لل Oxygen فبتبدأ ايش انه نسبة ال
+
+404
+00:30:01,710 --> 00:30:07,590
+RBCs تبعتهم اتقل ف ال hematocrit بيحتل من 30 ل 43%
+
+405
+00:30:07,590 --> 00:30:12,280
+من حجم ال holy bloodطبعا يا بنات هذا في العينة و
+
+406
+00:30:12,280 --> 00:30:15,220
+يمثل ال blood مش أنا أخدت عينة ممثلة عن ال blood
+
+407
+00:30:15,220 --> 00:30:19,600
+أخدت whole blood من الوريد و الله أخدت 2 cm و لا
+
+408
+00:30:19,600 --> 00:30:24,260
+أخدت 3 cm و لا 4 cm هم بيقش في محتوهم هيعبرلي عن
+
+409
+00:30:24,260 --> 00:30:32,170
+محتوى ال blood كله داخل الجسم طبعناف 30% ل 43%
+
+410
+00:30:32,170 --> 00:30:39,110
+adult male من 42% ل 52% ال adult female من 37% ل
+
+411
+00:30:39,110 --> 00:30:44,350
+47% why ال male أعلى؟ ليش ال adult male أعلى في ال
+
+412
+00:30:44,350 --> 00:30:49,270
+hemotocrit من ال adult female؟ أول إشي لإنه نسبة
+
+413
+00:30:49,270 --> 00:30:53,290
+ال muscle mass الكتلة العضلية عند ال .. ال .. ال
+
+414
+00:30:53,290 --> 00:30:57,740
+male أكترطبعا فهم محتاجين طالما عندهم muscle mass
+
+415
+00:30:57,740 --> 00:31:00,540
+أكتر يعني عندهم خلايا أكتر يعني محتاجين oxygen
+
+416
+00:31:00,540 --> 00:31:06,390
+delivery أكتر فبالتالي ال RBCs عندهم أكترطيب ال
+
+417
+00:31:06,390 --> 00:31:11,790
+adult female نظرا لإنه أيضا نسبة ال RBC قليلة
+
+418
+00:31:11,790 --> 00:31:19,510
+عندهم هم في عندهم regular blood loss every month
+
+419
+00:31:19,510 --> 00:31:24,430
+كل شهر بيصير فيه فقدان لل blood عند ال female من
+
+420
+00:31:24,430 --> 00:31:29,340
+خلال ال menstruation تمام؟ف .. فبالتالي هذا اللي
+
+421
+00:31:29,340 --> 00:31:33,180
+عامل نظرا لإنه عندهم الكتلة العضلية أقل و عندهم
+
+422
+00:31:33,180 --> 00:31:37,760
+فقدان ل ال blood كل .. بشكل شهري فبالتالي ال .. ال
+
+423
+00:31:37,760 --> 00:31:43,280
+.. نسبة ال hematocrita عندهم قليلة، تمام؟ أقل من
+
+424
+00:31:43,280 --> 00:31:47,000
+ال adult male، بس هذا ال normal تبعهم يعني، طيب
+
+425
+00:31:48,750 --> 00:31:55,370
+خلّينا نشوف ال .. إذا هك إحنا بنكون شرحنا كل إشي
+
+426
+00:31:55,370 --> 00:31:59,870
+بتعلق بالتجربة مع ال normal ranges هلأ بنحكي عن ال
+
+427
+00:31:59,870 --> 00:32:04,750
+source of error يعني بعض الملاحظات و بعض ال
+
+428
+00:32:04,750 --> 00:32:08,650
+trickات اللي ممكن تعملي خلل أو تعملي error في
+
+429
+00:32:08,650 --> 00:32:12,770
+النتيجة أو تعطيني قراءة خاطئة لإنسبة ال hematocrit
+
+430
+00:32:13,400 --> 00:32:16,540
+خلينا بس نشوف بالأول هنا الصورة نناقش مع بعض
+
+431
+00:32:16,540 --> 00:32:20,940
+الصورة إذا كانت هذه هي ال normal range ل ال .. ال
+
+432
+00:32:20,940 --> 00:32:25,080
+hematocrit يعني نسبة ال RBCs فإنه أقل من ال .. من
+
+433
+00:32:25,080 --> 00:32:28,460
+ال normal لو كان شخص عنده ال .. يعني هاي ال .. هاي
+
+434
+00:32:28,460 --> 00:32:32,160
+ال reference ranges أنا بقارن بهدول، تمام؟ فإذا
+
+435
+00:32:32,160 --> 00:32:35,800
+كان شخص عنده نسبة ال hematocrit أقل من ال normal،
+
+436
+00:32:35,800 --> 00:32:42,640
+تمام؟ فمعناه هذا الشخص عنده anemiaفقر دم عنده،
+
+437
+00:32:42,640 --> 00:32:45,720
+عنده نسبة ال RBC's قليلة بالنسبة لل holy blood،
+
+438
+00:32:45,720 --> 00:32:50,000
+يعني الخلايا عنده قليلة، فبالتالي خلايا الدم ال
+
+439
+00:32:50,000 --> 00:32:53,940
+RBC's، ال oxygen delivery قليل، عنده hypoxia، عنده
+
+440
+00:32:53,940 --> 00:32:59,790
+فقر دم، أنيمياطب لو كانت نسبة ال RBCs أعلى من
+
+441
+00:32:59,790 --> 00:33:02,690
+الطبيعي، أعلى من ال normal، هاي نفترض أنه هذا ال
+
+442
+00:33:02,690 --> 00:33:07,350
+normal، أعلى منه، بسمي هذه الحالة polycythemia،
+
+443
+00:33:07,350 --> 00:33:12,000
+إيش يعني polycythemia؟ poly يعني عديدكلمة poly
+
+444
+00:33:12,000 --> 00:33:16,360
+عديد خدتوها أكيد في الحيوية مرت عليكم لما ذكر
+
+445
+00:33:16,360 --> 00:33:21,940
+عندكم ال polysaccharide و زي كلمة poly عديد، طبعا؟
+
+446
+00:33:21,940 --> 00:33:25,480
+طيب، polysaccharide، polypeptide، poly .. كذا، أي
+
+447
+00:33:25,480 --> 00:33:31,340
+poly فهو عديد معناها كلمة .. كلمة poly site جاية
+
+448
+00:33:31,340 --> 00:33:36,510
+من كلمة خلايةتمام? site جاي من كلمة خلاية .. site
+
+449
+00:33:36,510 --> 00:33:41,310
+.. cytology علم الخلاية .. site خلاية .. emia كأي
+
+450
+00:33:41,310 --> 00:33:46,790
+كلمة بتنتهي ب emia يعني في الدم، تمام؟ يعني زي عدي
+
+451
+00:33:46,790 --> 00:33:50,450
+.. خلاية عديدة في الدم، يعني زيادة عدد خلاية الدم
+
+452
+00:33:50,450 --> 00:33:55,660
+ال RBCsتمام؟ هاي معناه كلمة polycythemia طيب يا
+
+453
+00:33:55,660 --> 00:33:59,780
+بنات إيش يعني polycythemia؟ في بعض الحالات طبعا هي
+
+454
+00:33:59,780 --> 00:34:06,180
+مجموعة من الأمراض مش مرض واحد وبينتج عنه أن الجسم
+
+455
+00:34:06,180 --> 00:34:11,580
+يصنع خلايا RBCs زيادة عن الطبيعييعني مثلا لو الشخص
+
+456
+00:34:11,580 --> 00:34:14,620
+.. ممكن .. ممكن يكون الشخص نتيجة الظروف اللي بيعيش
+
+457
+00:34:14,620 --> 00:34:18,880
+فيها ممكن يصير عنده polycythemia ممكن نتيجة organ
+
+458
+00:34:18,880 --> 00:34:23,280
+dysfunction يعني مشكلة عنده في أحد أعضاء جسمه فأدت
+
+459
+00:34:23,280 --> 00:34:26,460
+إلى إنه الجسم راح يصنع polycythemia .. يعني راح
+
+460
+00:34:26,460 --> 00:34:30,520
+يصنع RBCs بالزيادة فيكون عنده polycythemia أو حالة
+
+461
+00:34:30,520 --> 00:34:34,360
+ال humor ورم يعني الخلايا بتتكثر بشكل مش طبيعي
+
+462
+00:34:35,110 --> 00:34:38,850
+تمام؟ طيب نبدأ نحكي عنهم واحدة واحدة بالدور ال ..
+
+463
+00:34:38,850 --> 00:34:42,450
+ال .. الظروف اللي بعيش فيها الإنسان الناس اللي
+
+464
+00:34:42,450 --> 00:34:46,070
+بعيشوا على المرتفعات نظرا لو وجود نقص في ال oxygen
+
+465
+00:34:46,070 --> 00:34:50,890
+جسمهم بيصنع خلايا RBCs بزيادة تمام؟ هذه حالة
+
+466
+00:34:50,890 --> 00:34:54,050
+polycythemia صح هم normal بس بيندرجوا تحت ال
+
+467
+00:34:54,050 --> 00:35:01,370
+polycythemia تمام؟و ال .. الناس اللي عندهم organ
+
+468
+00:35:01,370 --> 00:35:05,630
+dysfunction، اذا عند شخص عنده مشكلة بالقلب، القلب
+
+469
+00:35:05,630 --> 00:35:09,690
+هو مضخة الدم، هو اللي بيضخ الدم ل .. ل .. ل سائر
+
+470
+00:35:09,690 --> 00:35:13,630
+أعضاء الجسم، طبعا؟ طيب، فإذا في عندي مشكلة في
+
+471
+00:35:13,630 --> 00:35:16,590
+القلب يعني في عندي مشكلة في ضخ الدم يعني في عندي
+
+472
+00:35:16,590 --> 00:35:21,900
+مشكلة في توصيل ال oxygen، طبعا؟البون مارو اللي
+
+473
+00:35:21,900 --> 00:35:27,340
+بيصنعلي ال .. الدم مش راح يفهم انه المشكلة في ال
+
+474
+00:35:27,340 --> 00:35:33,340
+heart في القلب طبعا؟ لأ هو بس عنده مجسات بيحس نسبة
+
+475
+00:35:33,340 --> 00:35:37,620
+ال oxygen مادام في عندي أي خلل أدى الى نقص نسبة ال
+
+476
+00:35:37,620 --> 00:35:41,340
+oxygen في الدم بيبدأ يصنع RBC's وهو مغمض عيناه
+
+477
+00:35:41,340 --> 00:35:49,620
+طبعا؟ يعني هو مصنع ولا بيسأش زي .. يعني مغم عيناه
+
+478
+00:35:49,990 --> 00:35:54,650
+تمام؟ بيشتغلوا هو مغمض عنه، مش شايف من المشكلة بس
+
+479
+00:35:54,650 --> 00:35:59,900
+حاسس أنه مافيش oxygen، هذا هو البومرةنتيجة مشكلة
+
+480
+00:35:59,900 --> 00:36:02,960
+في القلب، يعني في ضخ الدم أو مشكلة في الرئتين، في
+
+481
+00:36:02,960 --> 00:36:08,540
+التنفس، في هذه الحالتين المحصلة hypoxia، إيش يعني
+
+482
+00:36:08,540 --> 00:36:14,380
+hypoxia؟ hypo قليل، oxia أكسوجين، تمام؟ فبالتالي
+
+483
+00:36:14,380 --> 00:36:19,500
+المحصلة نقص في الأكسوجين في الجسم فال bone مروحة
+
+484
+00:36:19,500 --> 00:36:24,170
+كان مصنع بيشتغل و هو مغمض عيناه، بس بيحسفي .. في
+
+485
+00:36:24,170 --> 00:36:28,890
+Oxygen كافي بيبطل يصنع، مش Oxygen كافي بيصير يصنع،
+
+486
+00:36:28,890 --> 00:36:31,850
+عندي مشكلة في القلب أو في الرئتين، راح تخلي ال
+
+487
+00:36:31,850 --> 00:36:37,270
+bone marrow انه يحس انه مافي Oxygen كافي وبالتالي
+
+488
+00:36:37,270 --> 00:36:42,750
+معناها انه راح يصنع خلايا RBCs بزيادة و المحصلة
+
+489
+00:36:42,750 --> 00:36:47,320
+Polycythemiaالحالة التالتة أو النوع التالت من ال
+
+490
+00:36:47,320 --> 00:36:51,740
+polycythemia أنه يكون في عند تيومر الخلايا الأم لل
+
+491
+00:36:51,740 --> 00:36:55,320
+RBCs اللي موجودة في البون مارو طبعا كل نوع من
+
+492
+00:36:55,320 --> 00:36:59,940
+أنواع خلايا الدم يلو خلايا أم في البون مارو خلايا
+
+493
+00:36:59,940 --> 00:37:05,920
+من .. بس تتكثر بتنتج ليه هذا النوع طبعاالخلاق اللي
+
+494
+00:37:05,920 --> 00:37:09,300
+أم لل RBCs اللي موجودة في ال bone marrow صار فيها
+
+495
+00:37:09,300 --> 00:37:13,380
+خلل طفرة معينة أدت إلى إن هي بتتكاتر بشكل مجنون
+
+496
+00:37:13,380 --> 00:37:17,680
+فبت المحصلة إنه إنتاج المزيد من ال RBCs above
+
+497
+00:37:17,680 --> 00:37:20,740
+normal أكتر من أعلى من ال normal فيعني
+
+498
+00:37:20,740 --> 00:37:24,960
+polycythemiaإذا هي مجموعة من ال disorder و كلهم
+
+499
+00:37:24,960 --> 00:37:28,700
+بندرجوا تحت هذا الإسم، تمام؟ فهي معناه كلمة
+
+500
+00:37:28,700 --> 00:37:31,860
+polycythemia اللي بدي أكم تعرفوا انه hematocrite
+
+501
+00:37:31,860 --> 00:37:37,880
+lower than normal anemia hematocrite above than
+
+502
+00:37:37,880 --> 00:37:42,580
+normal or more than normal polycythemia
+
+503
+00:37:45,160 --> 00:37:49,460
+طيب فال .. اذا هذا هي .. هاي .. هذا التفسير الصورة
+
+504
+00:37:49,460 --> 00:37:54,320
+طيب أول خطوة أو أول نقطة في source of error and
+
+505
+00:37:54,320 --> 00:37:58,540
+comments بيحكيلي عن الimproper sealing يعني لو أنا
+
+506
+00:37:58,540 --> 00:38:01,720
+ما سكرتش كويس ال capability و كل شوية .. من الشوية
+
+507
+00:38:01,720 --> 00:38:05,360
+كنا عم نحكي عنهاحكينا إنه إيش راح يصير؟ راح يصير
+
+508
+00:38:05,360 --> 00:38:09,300
+بيه decrease لل hematocrit Why؟ لإنه في عندي loss
+
+509
+00:38:09,300 --> 00:38:13,660
+لل blood during centrifugation Why؟ لإنه احنا
+
+510
+00:38:13,660 --> 00:38:18,260
+ماسكرناش منيح فأثناء الطرد المركزي صار في عندي طرد
+
+511
+00:38:18,260 --> 00:38:22,620
+للخلايا باتجاه .. باتجاه منطقة مش مسكرة منيح، يعني
+
+512
+00:38:22,620 --> 00:38:27,360
+تثريب لل bloodونظرا لإنه الخلايا ال RBCs هي اللي
+
+513
+00:38:27,360 --> 00:38:32,080
+بتروح بهذا الاتجاه لإنها هي أثقل فاحنا بنفقد RBCs
+
+514
+00:38:32,080 --> 00:38:36,780
+أكتر من ال plasma فطالما أنا بقيس القراءة عندي
+
+515
+00:38:36,780 --> 00:38:39,460
+تبعت هي نسبة ال RBCs إذا راح يكون في عندي
+
+516
+00:38:39,460 --> 00:38:43,140
+decreased hematocritإذن a higher number of
+
+517
+00:38:43,140 --> 00:38:46,980
+erythrocyte are lost in relation to the plasma
+
+518
+00:38:46,980 --> 00:38:51,140
+نسبة ال RBCs اللي أنا بفقدها بالنسبة للبلازمة أكتر
+
+519
+00:38:51,140 --> 00:38:56,300
+لإنها هي الأثقل هي اللي بتتجه باتجاه من .. باتجاه
+
+520
+00:38:56,300 --> 00:38:59,960
+ال .. المنطقة اللي .. اللي المفروض تكون مسكرة إذا
+
+521
+00:38:59,960 --> 00:39:03,320
+مش مسكرة منيح راح يصير فقدان لهذه ال erythrocyte
+
+522
+00:39:03,320 --> 00:39:10,150
+إذا أنا عم بقرأ نسبة و فيها فاقد تمام؟طيب an
+
+523
+00:39:10,150 --> 00:39:13,870
+increased amount of anticoagulant decreased the
+
+524
+00:39:13,870 --> 00:39:16,670
+hematocrit reading as a result of erythrocyte
+
+525
+00:39:16,670 --> 00:39:21,110
+shrinkage ايش معنى الكلام هذا؟ احنا لاحظتوا انه
+
+526
+00:39:21,110 --> 00:39:27,170
+انا ركزت كتير اول المحاضرة على موضوع انه اذا باخد
+
+527
+00:39:27,170 --> 00:39:32,130
+عينة من skin puncture على طول مباشرة بدي اخد
+
+528
+00:39:32,130 --> 00:39:36,050
+hiperionized capillary ال red top او ال red cap
+
+529
+00:39:36,760 --> 00:39:42,240
+capillary إذا أنا باخد عينة من وريد و العينة
+
+530
+00:39:42,240 --> 00:39:45,740
+فرقتها في إيدي تتيوب بعدين بدي أعبي capillary بدي
+
+531
+00:39:45,740 --> 00:39:50,020
+أملأ capillary من هاي ال إيدي تتيوب طبعا فالمفروض
+
+532
+00:39:50,020 --> 00:39:54,880
+انه ال .. ال .. ال capillary هاي تكون plain يعني
+
+533
+00:39:54,880 --> 00:40:01,920
+blue cap او blue top طبعاوحكيتلكم إنه ممنوع أستخدم
+
+534
+00:40:01,920 --> 00:40:06,280
+الهيبرينيز من tube edita ممنوع أمرر البلد على
+
+535
+00:40:06,280 --> 00:40:10,080
+نوعين من ال anticoagulant لإن ال anticoagulants
+
+536
+00:40:10,080 --> 00:40:14,680
+هذه عبارة عن أملاح و الأملاح هاي بتأثر على ال
+
+537
+00:40:14,680 --> 00:40:18,970
+osmolality و ال osmotic pressure ل الخلاياطبعا
+
+538
+00:40:18,970 --> 00:40:23,950
+خلّينا بس نشوف مع بعض هذه الصورة لحتى تتضح الصورة
+
+539
+00:40:23,950 --> 00:40:32,410
+أكتر لحتى تتضح الصورة عندي خلايا دم وجدت في وسط أو
+
+540
+00:40:32,410 --> 00:40:37,090
+في محلول بسميه hypertonic إيش يعني hypertonic؟
+
+541
+00:40:37,090 --> 00:40:41,590
+يعني نسبة الأملح فيها عالية، طبعا؟ يعني blood
+
+542
+00:40:41,590 --> 00:40:46,130
+موجود في إيديتا وهيبرين كمان، نسبة الأملح عالية
+
+543
+00:40:46,130 --> 00:40:51,250
+حوالين الخلايافاحنا عارفين ان ال .. ال water اللي
+
+544
+00:40:51,250 --> 00:40:56,430
+موجود داخل هذه الخلايا .. ال water اللي موجود داخل
+
+545
+00:40:56,430 --> 00:41:02,850
+هذه الخلاياعارفين إن هو بينتقل من الضغط الاسموزي
+
+546
+00:41:02,850 --> 00:41:07,250
+القليل للضغط الاسموزي العالي يعني من تركيز أملح
+
+547
+00:41:07,250 --> 00:41:12,370
+قليل لتركيز أملح عالي فأصبح ال water هذا الماء
+
+548
+00:41:12,370 --> 00:41:16,050
+اللي موجود داخل الخلايا إيش راح يصير فيه؟ راح
+
+549
+00:41:16,050 --> 00:41:21,090
+ينتقل أو يخرج من الخلايا لحتى يحاول إنه يعادل أو
+
+550
+00:41:21,090 --> 00:41:25,130
+يخفف نسبة الأملح اللي موجودة حوالين الخلايا اللي
+
+551
+00:41:25,130 --> 00:41:30,960
+هي ال edit و الhebrine مع بعضطبعا؟ فالمحصلة الخلية
+
+552
+00:41:30,960 --> 00:41:35,520
+لما بتفقد ما إيش بتصير؟ بتكرمش طبعا؟ فبيصير فيها
+
+553
+00:41:35,520 --> 00:41:40,520
+shrinkage بيصير فيها حاجة اسمها shrinkage طبعا؟
+
+554
+00:41:40,520 --> 00:41:45,100
+الخلية لو كان ضغط الأملاح أو ضغط الاسموزي أو نسبة
+
+555
+00:41:45,100 --> 00:41:51,180
+الأملاح حوالين الخلايا مساوية ل .. ال .. ال ..نسبة
+
+556
+00:41:51,180 --> 00:41:56,240
+الأملاح داخل الخلايا إذا الماء هيكون حركته منتظمة
+
+557
+00:41:56,240 --> 00:42:01,080
+من و إلى الخلية إذا الخلية هتبقى intact، سليمة،
+
+558
+00:42:01,080 --> 00:42:04,200
+تمام؟ intact يعني كلمة .. كلمة intact معناها أن
+
+559
+00:42:04,200 --> 00:42:10,330
+الخلايا سليمة، بالشكل السليمتمام لو كان في عندي ال
+
+560
+00:42:10,330 --> 00:42:13,970
+.. ال .. طبعا هذا المحلول اللي بيكون فيه انتظام في
+
+561
+00:42:13,970 --> 00:42:19,610
+نسبة الأملح أو نسبة الأملح في مساوية لتركيزها داخل
+
+562
+00:42:19,610 --> 00:42:27,020
+الخلايا بسمي isotonic solution تمام؟ طيبمحلول نسبة
+
+563
+00:42:27,020 --> 00:42:34,080
+الأملاح فيه أقل من داخل الخلايا بسمي hybotonic إذا
+
+564
+00:42:34,080 --> 00:42:39,060
+الأملاح خارج الخلايا أكتر من داخلها hypertonic
+
+565
+00:42:39,060 --> 00:42:44,340
+أكتر hyper تمام؟ محلول نسبة الأملاح خارج الخلايا
+
+566
+00:42:44,340 --> 00:42:50,260
+مساوية لداخلها بسمي isotonic solutionمحلول نسبة
+
+567
+00:42:50,260 --> 00:42:55,500
+الأملاح فيه أقل من داخل الخلايا أو الوسط يعني
+
+568
+00:42:55,500 --> 00:43:01,140
+الوسط للخلية في نسبة الأملاح أقل من داخلها بسميها
+
+569
+00:43:01,140 --> 00:43:07,260
+hypotonic أقل hypoطيب، مدام نسبة الأملع حوالين
+
+570
+00:43:07,260 --> 00:43:11,060
+الخلايا أقل، معناها كيف هتصير حركة الماء؟ هتصير
+
+571
+00:43:11,060 --> 00:43:14,120
+حركة الماء من التركيز الأقل إلى داخل الخلايا،
+
+572
+00:43:14,120 --> 00:43:18,920
+التركيز الأعلى هيكون هو داخل الخلايا، تمام؟ فإنه
+
+573
+00:43:18,920 --> 00:43:26,720
+نظرا لإن الماء بيتحرك إلى داخل الخلية، معناها إنه
+
+574
+00:43:26,720 --> 00:43:31,320
+ممكن يدخل إلى الخلية ماءأكثر من طاقتها
+
+575
+00:43:31,320 --> 00:43:36,000
+الاستيعابية، مما يؤدي إلى إيش؟ انفجار الخلايا،
+
+576
+00:43:36,000 --> 00:43:42,600
+license للخلايا، تكسر breakdown للخلايا، تمام؟
+
+577
+00:43:42,600 --> 00:43:47,820
+طيب، هاي في عندي كمان صورة تانية لحتى تتضح الفكرة
+
+578
+00:43:47,820 --> 00:43:53,510
+أكترطبعا هاي محلول hypertonic إيش يعني؟ يعني
+
+579
+00:43:53,510 --> 00:43:57,850
+الأملح حوالين الخلايا .. هاي الخلايا اللي لونها
+
+580
+00:43:57,850 --> 00:44:02,470
+أحمر الأملح حوالين الخلايا أكثر من داخلها طبعا
+
+581
+00:44:02,470 --> 00:44:06,170
+أكثر من داخلها شايفين؟ هاي الدائرة هذه .. هاي
+
+582
+00:44:06,170 --> 00:44:09,730
+الدائرة شايفين؟ فيها نسبة أملح اللي لونهم أزرق
+
+583
+00:44:09,730 --> 00:44:14,130
+هدول طبعا أكتر من الأملح اللي موجودة داخل الخلية
+
+584
+00:44:14,130 --> 00:44:23,810
+إيش معناها بدو يصير يا بنات؟معناها بدو ال .. ال ..
+
+585
+00:44:23,810 --> 00:44:28,810
+ايش اللي بدو يصير؟ بدو يصير عندي الشرنكج تكرمش
+
+586
+00:44:28,810 --> 00:44:32,490
+للخلية هذه المصطلحات سيبوكم منها طبعا سيبوكم منها
+
+587
+00:44:32,490 --> 00:44:35,270
+هذه المصطلحات هلأ انا هكتبلكم ايش يعني كلمة
+
+588
+00:44:35,270 --> 00:44:39,790
+shrinkage و ايش يعني intact و .. و .. و licensed
+
+589
+00:44:39,790 --> 00:44:45,370
+بس المهم الفكرة طيب هي هذا الدائرة نسبة الأملح
+
+590
+00:44:45,370 --> 00:44:52,460
+فيهاأقل من اللي داخل الخلية، يعني hypotonic، ماشي،
+
+591
+00:44:52,460 --> 00:44:55,500
+معناها ال .. ال .. الخلية إيش راح يصير؟ راح تنتفخ،
+
+592
+00:44:55,500 --> 00:44:58,960
+تنتفخ، شايفين؟ هي انتفخت، انت تنتفخ، تنتفخ إلى ان
+
+593
+00:44:58,960 --> 00:45:03,540
+يصل فيها الماء أكثر من طاقتها الاستيعابية و بتقوم
+
+594
+00:45:03,540 --> 00:45:09,460
+.. بتنتفجرتمام؟ طيب isotonic يعني تركيز الأملح في
+
+595
+00:45:09,460 --> 00:45:12,960
+.. حوالين الخلايا مساوية تركيزها في الداخل فهي
+
+596
+00:45:12,960 --> 00:45:17,880
+يعني awesome، رائع، wow تمام؟ intact الخلية طيب،
+
+597
+00:45:17,880 --> 00:45:23,760
+خلّينا بس نلخص الاشي عشان أورجيكم ال .. الكلمات
+
+598
+00:45:23,760 --> 00:45:27,520
+يعني لو كانت ال .. ال .. الخلايا موجودة في
+
+599
+00:45:27,520 --> 00:45:31,280
+isotonic تمام؟ solution
+
+600
+00:45:35,280 --> 00:45:41,480
+معناها إيش رح يكون؟ رح تكون intact، سليمة، يعني
+
+601
+00:45:41,480 --> 00:45:46,960
+تركيز الأملح حواليها مساوية تركيز الأملح داخلها،
+
+602
+00:45:46,960 --> 00:45:51,260
+حركة الماء منتظمة من و إلى الخلية، الخلية هتظل
+
+603
+00:45:51,260 --> 00:45:58,060
+intact طيب، لو كان في عندي محلول hypertonic، تمام؟
+
+604
+00:45:58,060 --> 00:46:00,140
+hypertonic solution
+
+605
+00:46:04,350 --> 00:46:08,870
+hypertonic solution إيش معناها؟ معناها تركيز
+
+606
+00:46:08,870 --> 00:46:13,210
+الأملح حوالين الخلايا أكتر من تركيز الأملح داخلها
+
+607
+00:46:13,210 --> 00:46:19,870
+هذا إيش رح يقدي؟ رح يقدي إلى إنه الماء رح يخرج من
+
+608
+00:46:19,870 --> 00:46:25,970
+الخلية إلى خارجها يعني المحص لإن الخلية فقدت سائل
+
+609
+00:46:25,970 --> 00:46:32,510
+فقدت سائل إيش يعني؟ رح يصير فيها shrinkage رح يصير
+
+610
+00:46:32,510 --> 00:46:38,830
+فيها تكرمشطيب لو كان في عندي محلول صار فيه إيه؟ هو
+
+611
+00:46:38,830 --> 00:46:47,670
+عبارة عن hypotonic .. hypotonic solution، تمام؟
+
+612
+00:46:49,230 --> 00:46:53,170
+معناها هذا المحلول أو الوسط المحيط بالخلايا يا
+
+613
+00:46:53,170 --> 00:46:55,910
+بنات سواء محلول أو وسط المحيط يعني نفس المعنى
+
+614
+00:46:55,910 --> 00:46:59,790
+انتوا تخيلوها الوسط المحيط في الخلايا hypotonic
+
+615
+00:46:59,790 --> 00:47:03,850
+يعني تركيز الأملح فيه أقل من تركيزها داخل الخلية
+
+616
+00:47:03,850 --> 00:47:08,030
+طبعا؟ معناها المائش راح ينتقل إلى داخل الخلية لإن
+
+617
+00:47:08,030 --> 00:47:12,400
+التركيز العالي جوا الخليةتمام؟ هذا رح يقدي إلى
+
+618
+00:47:12,400 --> 00:47:17,880
+إيش؟ أول حاجة ال swell ل ال RBCs رح تنتفخ بعدين
+
+619
+00:47:17,880 --> 00:47:22,740
+إلى أن يصل يعني القمر يزيد .. يفيد عن حده و يصير
+
+620
+00:47:22,740 --> 00:47:29,840
+بعديها إيش؟ Lices تمام؟
+
+621
+00:47:29,840 --> 00:47:40,300
+Lices orbreak down تكسر طبعا او خلّيني على كلمة
+
+622
+00:47:40,300 --> 00:47:43,060
+license بلاش يعني break down ممكن تيجي مع ال
+
+623
+00:47:43,060 --> 00:47:46,400
+molecules أكتر من الخلايا فخليها على كلمة license
+
+624
+00:47:46,400 --> 00:47:52,950
+تكسر للخلايا طبعا؟ تحللهذا اللي بيصير في ال .. في
+
+625
+00:47:52,950 --> 00:47:58,290
+الأوساط المختلفة فإذا فكرة أنه ال blood يكون في
+
+626
+00:47:58,290 --> 00:48:02,830
+إيدي تتيوب و أروح أعبيه أو أملأه في hyperionized
+
+627
+00:48:02,830 --> 00:48:08,800
+capillary خطأ hypertonicنوعين من الملح، يعني في
+
+628
+00:48:08,800 --> 00:48:13,220
+عندي hypertonic صار، يعني shrinkage، يعني طالما ..
+
+629
+00:48:13,220 --> 00:48:16,860
+طب إيش يعني shrinkage؟ محصر يعني .. طب أنا قلي
+
+630
+00:48:16,860 --> 00:48:22,800
+ساعة بشرح .. إيش يعني shrinkage؟ معناهاإنه ال
+
+631
+00:48:22,800 --> 00:48:29,440
+RBC's مدام هتكرمشت راح تحتل حجم أصغر، خلايا مكرمشة
+
+632
+00:48:29,440 --> 00:48:33,200
+مش ماخدة الحجم الطبيعي تبعها، معناها الحجم اللي
+
+633
+00:48:33,200 --> 00:48:37,320
+بتحتله أصغر، إذا هيصير في عندي إيش؟ decreased of
+
+634
+00:48:37,320 --> 00:48:40,000
+the hematocrit، تمام؟ راح يصير في عندي decreased
+
+635
+00:48:40,000 --> 00:48:44,290
+of the hematocritفهمتوا يا بنات؟ إيش الفكرة؟ ليش
+
+636
+00:48:44,290 --> 00:48:48,410
+أنا ركزت على إنه أنا بختار الكابلة للصح علشان
+
+637
+00:48:48,410 --> 00:48:52,690
+أشتغل صح، تمام، طيب a decreased or increased
+
+638
+00:48:52,690 --> 00:48:56,210
+result may occur if the specimen was not properly
+
+639
+00:48:56,210 --> 00:49:00,330
+mixed برضه كمان يا بنات إذا أنا ما .. ماعملت
+
+640
+00:49:00,330 --> 00:49:04,450
+mixing منيح للعينة معناها يا إما أنا هقع في خطأ يا
+
+641
+00:49:04,450 --> 00:49:08,370
+إما hypertonic يا hypotonic يعني هي إما هيصير في
+
+642
+00:49:08,370 --> 00:49:11,190
+عند الشرنكش للخلايا فبالتالي decreased hematocrat
+
+643
+00:49:11,370 --> 00:49:15,070
+يعني ممكن يصير swell للخلايا انتفاخ فبيصير ..
+
+644
+00:49:15,070 --> 00:49:18,610
+بيصير increase أو حتى license يعني فقدان للخلايا
+
+645
+00:49:18,610 --> 00:49:24,300
+يعني decrease تمام؟ ده القراءةthe time and speed
+
+646
+00:49:24,300 --> 00:49:28,120
+of the centrifugation and the time when the
+
+647
+00:49:28,120 --> 00:49:30,620
+results are read are very important
+
+648
+00:49:53,150 --> 00:49:58,430
+فلازم يعني طبعا احنا كيف حددوها السرعة هاي و ال ..
+
+649
+00:49:58,430 --> 00:50:02,330
+و الوقت المناسب ل ال centrifugation من خلال عدة
+
+650
+00:50:02,330 --> 00:50:06,230
+دراسات وجدوا انه افضل وقت انه ال five minutes في
+
+651
+00:50:06,230 --> 00:50:08,850
+اعلى سرعة ما بين العشر الف الى خمستاشر الف minute
+
+652
+00:50:09,400 --> 00:50:13,460
+تمام؟ طيب ال insufficient centrifugation لو أنا
+
+653
+00:50:13,460 --> 00:50:17,960
+ماعملتش centrifugation كافى، إيش معناها؟ معناها إن
+
+654
+00:50:17,960 --> 00:50:23,260
+ال RBCs ماترسبتش بشكل كافى، إذا ممكن يكون تحتل حجم
+
+655
+00:50:23,260 --> 00:50:27,330
+أكبر من اللازم، إذا يصير فيه مشكلة في القراءةtime
+
+656
+00:50:27,330 --> 00:50:29,830
+of complete backing should be determined for each
+
+657
+00:50:29,830 --> 00:50:33,390
+centrifuge and rechecked at regular intervals كل
+
+658
+00:50:33,390 --> 00:50:36,270
+centrifuge بيجيني على المختبر يعني اذا انا مثلا
+
+659
+00:50:36,270 --> 00:50:38,990
+عطل عندي centrifuge اشتريت واحد جديد او استلمت
+
+660
+00:50:38,990 --> 00:50:45,990
+واحد جديد لازم انا اجري عدة تجارب لحتى احدد انه
+
+661
+00:50:45,990 --> 00:50:50,470
+يعني اعرف الوقت المناسب والسرعة المناسبة للفصل
+
+662
+00:50:51,060 --> 00:50:55,440
+طبعا؟ من خلال أن أنا مثلا أشتغل عينة عليه و
+
+663
+00:50:55,440 --> 00:51:01,260
+أشتغلها على جهاز آخر و أقارنهم مع بعضطيب the micro
+
+664
+00:51:01,260 --> 00:51:04,240
+hematocrit centrifuge should never be forced to
+
+665
+00:51:04,240 --> 00:51:08,580
+stop حكينا هذا في هذه النقطة واستفدنا حكينا ..
+
+666
+00:51:08,580 --> 00:51:13,220
+حكينا إنه ممنوع أنا أجبر الجهاز إنه يقف أو أوقفه
+
+667
+00:51:13,220 --> 00:51:18,260
+هيك بإيدي أو أحاول أبطق سرعته why؟ لإنه حكينا ال
+
+668
+00:51:18,260 --> 00:51:24,520
+.. ال .. يعني ممنوع أنا أجبره إنه يقف من خلال
+
+669
+00:51:24,520 --> 00:51:27,100
+applying pressure to the metal cover plate عند أنا
+
+670
+00:51:27,100 --> 00:51:32,870
+أحط إيدي أو يعني أعمل ضغط علىال .. الغطى طبعه this
+
+671
+00:51:32,870 --> 00:51:37,030
+will cause ليش حكينا لإنه هيخلى ال RBCs ليه؟ طبقة
+
+672
+00:51:37,030 --> 00:51:41,070
+ال RBCs اللي اترثبت راح يصيرلها sling تنفصل باتجاه
+
+673
+00:51:41,070 --> 00:51:43,810
+ال plasma طبعا راح يصيرلها forward إلى الأمام
+
+674
+00:51:43,810 --> 00:51:47,890
+باتجاه ال plasma و ال result راح ينتج عنها إيه؟
+
+675
+00:51:47,890 --> 00:51:51,530
+انه falsely elevated elevated يعني increased طبعا
+
+676
+00:51:51,530 --> 00:51:56,750
+resultطيب if too much time elapses between when
+
+677
+00:51:56,750 --> 00:51:59,170
+the centrifuge stop and the capillary tube is
+
+678
+00:51:59,170 --> 00:52:04,890
+removed هلأ إذا في وقت طويل مابان توقف ال
+
+679
+00:52:04,890 --> 00:52:08,030
+centrifuge و لما أنا روحت أفضيت و روحت أقرأ
+
+680
+00:52:08,030 --> 00:52:12,110
+النتيجة هذا أيضا ممكن يأثر على النتائج لإنه في هذا
+
+681
+00:52:12,110 --> 00:52:18,630
+الوقت ممكن تكون ال RBCs بدت تحل بدت يعني يبطل
+
+682
+00:52:18,630 --> 00:52:26,290
+ترسيب أو يعني sedimentationتمام فبالتالي ممكن انها
+
+683
+00:52:26,290 --> 00:52:29,990
+تتحرك ايضا باتجاه البلازم وهذا ممكن يعملي falsely
+
+684
+00:52:29,990 --> 00:52:32,010
+elevated hematocrit تمام
+
+685
+00:52:35,890 --> 00:52:39,130
+the buffy coat of a specimen shouldn't be included
+
+686
+00:52:39,130 --> 00:52:43,070
+أنا مش لازم أقرأ و أضمن ال buffy coat من ضمن
+
+687
+00:52:43,070 --> 00:52:45,670
+القراءة حكينا بقرأ من تحت ال .. ال .. ال buffy
+
+688
+00:52:45,670 --> 00:52:47,890
+coat shouldn't be included with the reading
+
+689
+00:52:47,890 --> 00:52:51,750
+because it's inclusion falsely elevated the result
+
+690
+00:52:51,750 --> 00:52:55,570
+يعني القراءة تبعته لو أنا بدي أدخل ال buffy coat
+
+691
+00:52:55,570 --> 00:52:58,890
+في القراءة تبعتي هذا هيرفعلي ال .. ال hematocrit
+
+692
+00:52:58,890 --> 00:53:02,980
+وهذا خطأa decrease or increase in the reading may
+
+693
+00:53:02,980 --> 00:53:06,320
+be seen if the microhematocrit reader is not used
+
+694
+00:53:06,320 --> 00:53:09,840
+properly إذا أنا ما استخدمت التدريج صح ممكن أيضا
+
+695
+00:53:09,840 --> 00:53:12,160
+يكون عندى فيه error في النتيجة decrease or
+
+696
+00:53:12,160 --> 00:53:18,210
+increase تمامركزوا معايا فى النقطة رقم 9 فى عندى
+
+697
+00:53:18,210 --> 00:53:23,870
+أمراض number of disorders عدد من الأمراض زى ال
+
+698
+00:53:23,870 --> 00:53:27,310
+sickles الانيميا انيميا الخلايا المنجالية اللى
+
+699
+00:53:27,310 --> 00:53:30,630
+بيكون فيها الخلايا ال RBC's ماخدة شكل ال .. ال ..
+
+700
+00:53:30,630 --> 00:53:33,810
+ال .. منجل شكل ال .. ال .. ال .. ال .. ال .. ال ..
+
+701
+00:53:33,810 --> 00:53:33,990
+ال .. ال .. ال .. ال .. ال .. ال .. ال .. ال .. ال
+
+702
+00:53:33,990 --> 00:53:34,290
+.. ال .. ال .. ال .. ال .. ال .. ال .. ال .. ال ..
+
+703
+00:53:34,290 --> 00:53:34,630
+ال .. ال .. ال .. ال .. ال .. ال .. ال .. ال .. ال
+
+704
+00:53:34,630 --> 00:53:34,950
+.. ال .. ال .. ال .. ال .. ال .. ال .. ال .. ال ..
+
+705
+00:53:34,950 --> 00:53:36,030
+ال .. ال .. ال .. ال .. ال .. ال .. ال .. ال .. ال
+
+706
+00:53:36,030 --> 00:53:38,790
+.. ال .. ال .. ال .. ال .. ال .. ال .. ال .. ال ..
+
+707
+00:53:38,790 --> 00:53:38,850
+ال .. ال .. ال .. ال .. ال .. ال .. ال .. ال .. ال
+
+708
+00:53:38,850 --> 00:53:42,450
+.. ال .. ال .. ال .. ال .. ال ..
+
+709
+00:53:46,230 --> 00:53:49,470
+hypochromic anemia الخلايا اللى بيكون فيها مشكلة
+
+710
+00:53:49,470 --> 00:53:51,750
+في ال homoglobin يعنى الخلايا بتكون فيها صغيرة
+
+711
+00:53:51,750 --> 00:53:55,930
+اصغر من الطبيعى spherocytosis بتكون الخلايا عاملة
+
+712
+00:53:55,930 --> 00:53:59,570
+زى شكل الكرة مش diskotic و bi concave احنا أخدنا
+
+713
+00:53:59,570 --> 00:54:03,950
+ان ال RBCs إذا طلعت عليها نظرة من فوقبشوفها
+
+714
+00:54:03,950 --> 00:54:09,630
+discotheque، side view بشوفها biconcave، صح؟ طيب،
+
+715
+00:54:09,630 --> 00:54:14,530
+فإذا ال .. ال .. لما أجي أنا أطلع عليها من النظرة
+
+716
+00:54:14,530 --> 00:54:17,870
+الجانبية، بشوفهاش biconcave، بشوفها دائرة، فيعني
+
+717
+00:54:17,870 --> 00:54:23,280
+هذه مخدة شكل الكرة, sphero sideثلاثيميا مرض
+
+718
+00:54:23,280 --> 00:54:27,020
+الانيميا البحر المتوسط وكلنا سمعنا عن الثلاثيميا
+
+719
+00:54:27,020 --> 00:54:32,160
+ومشكلة الثلاثيميا انه بيكون في تباين في شكل ال
+
+720
+00:54:32,160 --> 00:54:39,460
+RBCs طبعا هدولة يبنات الخلل في شكل ال RBCs انيميا
+
+721
+00:54:39,460 --> 00:54:43,340
+خلايا منجلية Sickle Cell انيميا يعني ال RBCs زي
+
+722
+00:54:43,340 --> 00:54:49,570
+شكل الأسشاب macrocytic انيميا خلايا كبيرةوحجم
+
+723
+00:54:49,570 --> 00:54:53,350
+الخلايا كبيرة يعني الخلية نفسها كبيرة hypochromic
+
+724
+00:54:53,350 --> 00:54:57,030
+anemia الخلايا .. الخلية صغيرة spherocytosis
+
+725
+00:54:57,030 --> 00:55:00,330
+الخلية مش مخدة شكل طبيعي مش dystopic ولا biconcave
+
+726
+00:55:00,330 --> 00:55:07,340
+مخدة شكل كروي تمام ثلاثيمياتباين في شكل الخلية هذا
+
+727
+00:55:07,340 --> 00:55:11,860
+رح يقدي أثناء ال .. ال .. الفصل رح يقدي أثناء
+
+728
+00:55:11,860 --> 00:55:15,160
+الفصل أثناء ال centrifugation إلى إنه جزء من اللي
+
+729
+00:55:15,160 --> 00:55:20,800
+بلازمة يصير له trapped ينحجز بين الخلايا وهذا رح
+
+730
+00:55:20,800 --> 00:55:25,300
+يأثر على القراءة تبعتيطيب عشان نفهم الفكرة هذه أنا
+
+731
+00:55:25,300 --> 00:55:31,040
+جبتلكم صورة عبارة عن كورات، تمام؟ خلينا لو نشوف
+
+732
+00:55:31,040 --> 00:55:35,260
+أنا عندي سلة و بدي أصفط فيها الكورات و تكون
+
+733
+00:55:35,260 --> 00:55:40,300
+الكورات بشكل منتظم، تمام؟ و نفس الحجم و نفس الشكل
+
+734
+00:55:40,300 --> 00:55:45,620
+سهل جدا أصفطهابين بعضها، تمام؟ لو جيت هذه الكورات
+
+735
+00:55:45,620 --> 00:55:48,540
+كبيت في .. في داخل السلق أو في داخل ال .. ال .. ال
+
+736
+00:55:48,540 --> 00:55:52,040
+container خلينا نفترض أنها هي داخل صندوق و حطيت
+
+737
+00:55:52,040 --> 00:55:57,400
+صفطتها بشكل منتظم على بعضها البعض و جيت أكب عليها
+
+738
+00:55:57,400 --> 00:56:03,060
+مثلا سائل، مية نسبة الماء اللي ممكن يحتجز داخل أو
+
+739
+00:56:03,060 --> 00:56:06,760
+ما بين الخلايا هتكون قليلة لإنها منتظمة لإن مافيش
+
+740
+00:56:06,760 --> 00:56:15,980
+فراغات ما بينها، تمام؟و نفس الفكرة هنا شايفين؟
+
+741
+00:56:15,980 --> 00:56:21,260
+هذي من لعبة ماخدينها، في لعبة الكورات، تمام؟ بس ان
+
+742
+00:56:21,260 --> 00:56:25,420
+انا أخدوا الفكرة منها، اتخيلي ان هذي ال RBC's
+
+743
+00:56:25,420 --> 00:56:29,060
+تبعتي كلها نفس الشكل و تقضي ان كلها لونها أحمر،
+
+744
+00:56:29,060 --> 00:56:32,910
+يعني كلها red blood cell، تمام؟لو أنا جيت داخل ال
+
+745
+00:56:32,910 --> 00:56:36,450
+cabillary tube و بعد ما عملتي لها centrifugation
+
+746
+00:56:36,450 --> 00:56:39,710
+صار لها sedimentation صفة اتواقع على بعضها بشكل
+
+747
+00:56:39,710 --> 00:56:44,230
+regular تمام؟ بشكل طبعا هيكون المسافات ما بينها
+
+748
+00:56:44,230 --> 00:56:49,410
+قليلة جدا يعني البلازمة يا بنات مش روح تنهجز بينها
+
+749
+00:56:49,410 --> 00:56:55,030
+مش روح تنهجز بينهاتمام؟ فإذا أنا النسبة اللي
+
+750
+00:56:55,030 --> 00:56:59,430
+بقرأها هذه .. نسبة اللي بقرأها .. نسبة الخلايا هي
+
+751
+00:56:59,430 --> 00:57:04,070
+فقط خلايا مش خلايا و plasma صح؟ يعني ال hemotocrit
+
+752
+00:57:04,070 --> 00:57:09,370
+هيكون بيعبرلي بشكل أدق عن نسبة ال RBCs لو كانت
+
+753
+00:57:09,370 --> 00:57:15,080
+الخلايا منتظمة و نفس الشكللكن لو كانت الخلايا منها
+
+754
+00:57:15,080 --> 00:57:20,600
+.. منها زي هيك .. زي هذا الشكل تمام و هي بينها
+
+755
+00:57:20,600 --> 00:57:24,520
+الأسشاب الخلايا اللي .. اللي عاملة .. ماخدة شكل
+
+756
+00:57:24,520 --> 00:57:27,960
+الأسشاب الخلايا المنجلية أو خلايا كبيرة و خلايا
+
+757
+00:57:27,960 --> 00:57:31,120
+صغيرة في ال macrocytic أو خلايا صغيرة و خلايا
+
+758
+00:57:31,120 --> 00:57:35,300
+طبيعية في الhypochromic أو خلايا ماخدة شكل قرصي أو
+
+759
+00:57:35,300 --> 00:57:38,680
+biconcave و خلايا ماخدة شكل كرة أو ان في عندي
+
+760
+00:57:38,680 --> 00:57:45,160
+تباين في أشكال الخلايازي هذا بالظبط خلينا بس نعرض
+
+761
+00:57:45,160 --> 00:57:51,520
+الصورة هاي نشوف افترض ان هدول خلايا RBCs متباينة
+
+762
+00:57:51,520 --> 00:57:55,200
+الشكل إش صغير، إش كبير، إش كروي، إش بيضاوي، إش
+
+763
+00:57:55,200 --> 00:58:00,000
+أسشاب و بدريك تحطيهم في container و تفصليهم مع بعض
+
+764
+00:58:00,000 --> 00:58:04,290
+عشان تاخد الحجمهيكون طبعا مش هيجلسوا على بعض مش
+
+765
+00:58:04,290 --> 00:58:08,010
+هيتصفطوا على بعض بانتظام فبالتالي هيكون في فراغات
+
+766
+00:58:08,010 --> 00:58:12,070
+ما بينهم سهل الفراغات هتتعبيها البلازما إذا أنا
+
+767
+00:58:12,070 --> 00:58:15,230
+لما بدأ أقرأ نسبة الخلايا ال volume اللي بتحتله
+
+768
+00:58:15,230 --> 00:58:21,110
+هذه الخلايا المتباينة هكون بقرأ RBCs وبيتخللها
+
+769
+00:58:21,110 --> 00:58:23,910
+بلازما إذا نسبة ال hemotocrit تبعتي يا بنات
+
+770
+00:58:23,910 --> 00:58:31,090
+إشمالها هتكون خطأنتيجة إيش؟ وجود trapped plasma
+
+771
+00:58:31,090 --> 00:58:35,250
+طبعا، ال plasma to be trapped، ال plasma انحجزت
+
+772
+00:58:35,250 --> 00:58:38,610
+بين الخلايا، الأفضل أن تكون الخلايا منتظمة عشان
+
+773
+00:58:38,610 --> 00:58:41,130
+مايكونش في بينها فرغات و مايقولي plasma ما تصير
+
+774
+00:58:41,130 --> 00:58:46,430
+لها trappingThe trapping of plasma يعني انه ينحجز
+
+775
+00:58:46,430 --> 00:58:50,290
+لبلازما داخل او ما بين في المسافات البينية بين ال
+
+776
+00:58:50,290 --> 00:58:54,650
+RBCs غير منتظمة هذا ممكن يخلي ان ال hematocrit
+
+777
+00:58:54,650 --> 00:58:59,890
+تبعي يكون 1 ل 3% higher than the obtained of the
+
+778
+00:58:59,890 --> 00:59:03,280
+automated instrumentطبعا يعني لو أنا حطيته على
+
+779
+00:59:03,280 --> 00:59:09,060
+جهاز .. جهاز ال CBC و حسبت hematocrit طبعا و قرأته
+
+780
+00:59:09,060 --> 00:59:11,940
+بالتجربة اللي احنا شرحناها بتجربة ال micro
+
+781
+00:59:11,940 --> 00:59:15,240
+hematocrit هلاحظ ان التجربة بتعطيني نسبة
+
+782
+00:59:15,240 --> 00:59:18,520
+hematocrit أعلى من 1 ل 3% يعني لو جهاز ال CBC
+
+783
+00:59:18,520 --> 00:59:25,620
+أعطاني القيمةخمسة و تلاتين تمام على جهاز ال CPC
+
+784
+00:59:25,620 --> 00:59:30,160
+ممكن القراقة باعتى بالاستخدام ال Cabillary Tube
+
+785
+00:59:30,160 --> 00:59:33,100
+تكون ستة و تلاتين أو سبعة و تلاتين أو تمانية و
+
+786
+00:59:33,100 --> 00:59:39,930
+تلاتينطبعا من 1 ل 3% أعلى ليش؟ لإن في نسبة trapped
+
+787
+00:59:39,930 --> 00:59:44,870
+plasma و أنا لما قرأت قرأت ال RBCs الغير منتظمة
+
+788
+00:59:44,870 --> 00:59:49,230
+هذه و ال plasma المحجوزة بينها إذا زادت نسبة ال
+
+789
+00:59:49,230 --> 00:59:52,550
+hematocrit عندي فالأفضل إن الناس اللي عندهم
+
+790
+00:59:52,550 --> 00:59:56,290
+الأمراض هذه ما أشتغللهم ال hematocrit بطريقة ال
+
+791
+00:59:56,290 --> 01:00:01,350
+capillary tubeأشتغله بجهاز ال CBC لإنه جهاز ال CBC
+
+792
+01:00:01,350 --> 01:00:05,290
+بيعمله .. بيعمله calculation و ليس measurement
+
+793
+01:00:05,290 --> 01:00:12,190
+بيحسب ال .. ال hematocrit من ال .. من حجم ال RBC و
+
+794
+01:00:12,190 --> 01:00:17,470
+عددها تمام؟ يعني بيطبق قانون معين و بعدين بي ..
+
+795
+01:00:17,470 --> 01:00:21,690
+بيحسب ليه نسبة ال hematocrit إذا ما بتأثر قيمة ال
+
+796
+01:00:21,690 --> 01:00:24,890
+hematocrit اللي جاية من الجهاز من جهاز ال CBC ما
+
+797
+01:00:24,890 --> 01:00:30,030
+بتتأثر بال trapped plasmaطيب فإذا هذه فكرة trapped
+
+798
+01:00:30,030 --> 01:00:33,310
+plasma الناس اللي عندهم الأمرض هدولة بقرألهم على
+
+799
+01:00:33,310 --> 01:00:36,850
+جهاز ال CBC مابشتغل بطريقة micro hematocrit tube،
+
+800
+01:00:36,850 --> 01:00:40,910
+capillary tube طيب a temporarily low hematocrit
+
+801
+01:00:40,910 --> 01:00:45,490
+reading may result immediately after blood loss لو
+
+802
+01:00:45,490 --> 01:00:51,310
+شخص عنده نزيفسال عنده blood loss، معناها راح يكون
+
+803
+01:00:51,310 --> 01:00:54,530
+في عنده low hematocrit، نتيجة فقدان ال .. ال .. ال
+
+804
+01:00:54,530 --> 01:01:03,140
+blood، نتيجة فقدان ال .. الخلاياأول اشي السبب في
+
+805
+01:01:03,140 --> 01:01:06,200
+ال low hematocrit انه فقد خلايا سفي عنده blood
+
+806
+01:01:06,200 --> 01:01:12,240
+loss نظيف السبب التانى انه الجسم لما بده يعود
+
+807
+01:01:12,240 --> 01:01:17,720
+بيعود ل plasma أسرع مام بعود الخلايا فهيكون في
+
+808
+01:01:17,720 --> 01:01:22,860
+عندى لفترة مؤقتة بعد النظيف حتى لو توقف هيضل انه
+
+809
+01:01:22,860 --> 01:01:26,140
+في عنده لفترة مؤقتة ال hematocrit هيضل له إلى ان
+
+810
+01:01:26,140 --> 01:01:29,300
+الجسم لبين ما ياخد وقته في تعويض ال erythrocyte
+
+811
+01:01:29,300 --> 01:01:35,000
+اللى تم فقدانهاطيب proper specimen collection is
+
+812
+01:01:35,000 --> 01:01:38,240
+important consideration إن أنا أسحب العينة بشكل صح
+
+813
+01:01:38,240 --> 01:01:42,960
+يا بنات برضه كمان بيأثر على النتيجة اتخيلي سحبتي
+
+814
+01:01:42,960 --> 01:01:46,240
+من منطقة فيها hematoma يعني سحبتي hemolized blood
+
+815
+01:01:46,240 --> 01:01:49,880
+blood مكسر يعني ال hematoma هيصير له decreased
+
+816
+01:01:49,880 --> 01:01:56,320
+اتخيلي انت سحبتي ال .. ال .. ال .. ال ..ال tube ..
+
+817
+01:01:56,320 --> 01:02:00,880
+ال blood و مافضطيها كويس فى ال tube فمعناها صار فى
+
+818
+01:02:00,880 --> 01:02:03,860
+clot يعنى فى صار عندى consuming فى ال herbicides
+
+819
+01:02:03,860 --> 01:02:08,860
+معناها هتتأثر النتيجة تخيلي أنه انت سحبت العينة و
+
+820
+01:02:08,860 --> 01:02:12,620
+حطيها فى إيدي تا tube بعدين ملقته hiperionized
+
+821
+01:02:12,880 --> 01:02:15,660
+cabulary معناها صار في عندي shrinkage في الخلايا
+
+822
+01:02:15,660 --> 01:02:19,980
+نتيجة انه في عندي double anticoagulant نوعين من ال
+
+823
+01:02:19,980 --> 01:02:22,380
+anticoagulant معناها يصير فيه decrease في ال
+
+824
+01:02:22,380 --> 01:02:28,020
+hematocrat اذا طريقة جمع العينة و اجراء الفحص مهمة
+
+825
+01:02:28,020 --> 01:02:33,720
+جدا لحتى احصل على representative results او
+
+826
+01:02:33,720 --> 01:02:40,290
+accurate and precise resultنتائج ممثلة معبرة عن
+
+827
+01:02:40,290 --> 01:02:46,950
+حالة الشخص اللى انا عم بشتغله الفحص او يعني نتائج
+
+828
+01:02:46,950 --> 01:02:50,350
+accurate and precise صحيحة و دقيقة the
+
+829
+01:02:50,350 --> 01:02:53,490
+introduction of interstitial fluid from skin لو
+
+830
+01:02:53,490 --> 01:02:57,390
+انا تخيلي ب .. بدي اسحب عينة ال skin puncture
+
+831
+01:02:57,390 --> 01:03:03,090
+ويصيرت اعصر فيها اش يعني؟عصرت فيها حكينا إنه هيصير
+
+832
+01:03:03,090 --> 01:03:07,150
+في عندي tissue juice عصارة الخلايا هتطلع مع ال
+
+833
+01:03:07,150 --> 01:03:11,810
+blood يعني أنا باخد diluted blood مع إني أخدت
+
+834
+01:03:11,810 --> 01:03:15,210
+diluted blood طبيعي إنه نسبة ال RBC هتقل لإنها
+
+835
+01:03:15,210 --> 01:03:22,150
+تخففت يعني decreased hematocrit reading تمام؟طيب
+
+836
+01:03:22,150 --> 01:03:26,370
+أخر نقطة في المحاضرة اللي هي rule of three قانون
+
+837
+01:03:26,370 --> 01:03:30,110
+التلاتة بحكيلك أنه من نسبة ال hematocrit اللي أنت
+
+838
+01:03:30,110 --> 01:03:35,350
+بتحسبيها ممكن تجيبي ال hemoglobin تمام؟ تحسبي نسبة
+
+839
+01:03:35,350 --> 01:03:41,030
+ال hemoglobin يعني قوة الدم تمام؟ بالبلد من القرأة
+
+840
+01:03:41,030 --> 01:03:44,790
+ال hematocritمن خلال تطبيق ال rule of three ولكن
+
+841
+01:03:44,790 --> 01:03:47,850
+هاي القانون يا بنات أنا بس بطبقه عن الناس ال
+
+842
+01:03:47,850 --> 01:03:50,550
+normal هو مابيكونش صالح ان اطبقه عن الناس ال
+
+843
+01:03:50,550 --> 01:03:53,710
+abnormal و اللي عندها مشاكل يعني لو شخص normal
+
+844
+01:03:53,710 --> 01:04:01,150
+طبيعي و كان مثلا ال hematocrit تبعه اتناش ال
+
+845
+01:04:01,150 --> 01:04:06,210
+hematocrit تبعه ستة و تلاتين تمام؟ ستة و تلاتين
+
+846
+01:04:07,920 --> 01:04:12,560
+بحكيلك انه الهموجلبين هو ال hematocrit على تلاتة
+
+847
+01:04:12,560 --> 01:04:18,420
+plus minus تلاتة طبعا خلينا
+
+848
+01:04:18,420 --> 01:04:24,800
+انا اكتب القانون هنا الهموجلبين يا بنات يساوي ال
+
+849
+01:04:24,800 --> 01:04:30,440
+hematocrit على
+
+850
+01:04:30,440 --> 01:04:32,320
+تلاتة
+
+851
+01:04:38,480 --> 01:04:46,500
+اللي هي ماتكرت على تلاتة plus minus plus
+
+852
+01:04:46,500 --> 01:04:52,400
+minus يعني مش عارفة انا اطلعها من ال keyboard plus
+
+853
+01:04:52,400 --> 01:04:59,500
+minus بس انتوا يعني هيها لحظة خليني أجيبها من هنا
+
+854
+01:04:59,500 --> 01:05:04,840
+plus
+
+855
+01:05:04,840 --> 01:05:10,530
+minus تلاتةطبعا لو كان عندي الهيمتوكرت مثلا طلعته
+
+856
+01:05:10,530 --> 01:05:15,010
+36 أعطتكم مثال معناه الهيمتوكرت على تلاتة ستة و
+
+857
+01:05:15,010 --> 01:05:19,670
+تلاتين على تلاتة اتناش طبعا اتناش plus minus تلاتة
+
+858
+01:05:19,670 --> 01:05:23,890
+يعني ال homoglobin بدو يكون من اتناش ناقص تلاتة
+
+859
+01:05:23,890 --> 01:05:29,910
+يعني من تسعة لاتناش plus تلاتة لخمستاش جرام per
+
+860
+01:05:29,910 --> 01:05:33,870
+deciliter هذه واحدة قياس ال homoglobinهذا في حال
+
+861
+01:05:33,870 --> 01:05:37,030
+لو فرضت إن الهموجلبين تلاتة بطلعوا fringe بنات مش
+
+862
+01:05:37,030 --> 01:05:44,970
+قيمة طبعا طيب طب لو أنا بعرف الهموجلبين بس ما بعرف
+
+863
+01:05:44,970 --> 01:05:49,630
+ال hematocrat عادي سهل جدا والله إن ال hematocrat
+
+864
+01:05:49,630 --> 01:05:54,790
+يساوي الهموجلبين
+
+865
+01:05:54,790 --> 01:06:05,850
+ضرب تلاتة plus minus تلاتةلو أنا ب .. بعرف نفرض
+
+866
+01:06:05,850 --> 01:06:11,590
+إنه اللي هم جلبين عشرة plus .. ضرب تلاتة يعني
+
+867
+01:06:11,590 --> 01:06:15,530
+تلاتين plus minus تلاتين يعني من سبعة و عشرين
+
+868
+01:06:15,530 --> 01:06:20,890
+لتلاتة و تلاتين في المية ال hemotocrit طبعا؟ طيب
+
+869
+01:06:20,890 --> 01:06:25,920
+في ممكن انا .. اذا حكينا انا ال hemotocritبعبر عنه
+
+870
+01:06:25,920 --> 01:06:30,520
+بال percentage النسبة المقاوية و ممكن انا ما اعبر
+
+871
+01:06:30,520 --> 01:06:35,820
+عنه بالنسبة المقاوية اعبر عنه بال letter تمام
+
+872
+01:06:35,820 --> 01:06:43,070
+letter bear letter طيب letter bear letterيعني لو
+
+873
+01:06:43,070 --> 01:06:48,350
+كان ال hematocrit 36 في المية كبدأ أحولها لوحدة
+
+874
+01:06:48,350 --> 01:06:52,310
+letter per letter بقسمها عالميا ال 36 بدها تصيراش
+
+875
+01:06:52,310 --> 01:06:57,870
+36 من 100 letter per letter طبعا هذه بنات letter
+
+876
+01:06:57,870 --> 01:07:00,550
+per letter بتروح مع بعضها يعني انا هيك بس حطاها
+
+877
+01:07:00,550 --> 01:07:05,500
+decorطبعا و الفكرة يعني هو بيعبر عنها بال
+
+878
+01:07:05,500 --> 01:07:08,080
+percentage بس انا ممكن يعني اعبر عنها بال letter
+
+879
+01:07:08,080 --> 01:07:11,240
+per letter بس اقسم القيمة على 100 عشان تخلص من
+
+880
+01:07:11,240 --> 01:07:15,420
+النسبة المئوية هذه طبعا اذا عشان لما تشوفوا قيمة
+
+881
+01:07:15,420 --> 01:07:18,920
+ال hemotocrit ماعطيكم مياها بالقيمة هذه سهل جدا
+
+882
+01:07:18,920 --> 01:07:23,050
+تحولوها لنسبة مئوية و تفهموش يعني الكلام هذاهي هنا
+
+883
+01:07:23,050 --> 01:07:27,510
+معطيني انه when specimens are analyzed by either
+
+884
+01:07:27,510 --> 01:07:31,490
+automated or manual method a quick visual check of
+
+885
+01:07:31,490 --> 01:07:34,470
+the result of hemoglobin and hematocrit can be
+
+886
+01:07:34,470 --> 01:07:36,910
+done لو انا اشتغلت ال hemoglobin او ال hematocrit
+
+887
+01:07:36,910 --> 01:07:41,330
+بسواق automated او manual ممكن سهل جدا اطلع عليهم
+
+888
+01:07:41,330 --> 01:07:45,010
+و اشوف هل هم a blind بيطبقوا ال rule of three
+
+889
+01:07:45,010 --> 01:07:48,210
+بيطبقوا قانون التلاتة اللي هو اش بيحكي قانون
+
+890
+01:07:48,210 --> 01:07:54,490
+التلاتة انه ال hemoglobin يساويsorry أنه ال
+
+891
+01:07:54,490 --> 01:07:57,530
+homoglobin بيساوي ال hemotocrit على تلاتة plus
+
+892
+01:07:57,530 --> 01:08:00,690
+minus تلاتة أو ال hemotocrit يساوي ال homoglobin
+
+893
+01:08:00,690 --> 01:08:04,990
+ضارب تلاتة plus minus تلاتة طبعا و هاي هنا يعطيني
+
+894
+01:08:04,990 --> 01:08:13,380
+مثال لو كان عندك أنه ال homoglobin12 طبعا فهي تطبق
+
+895
+01:08:13,380 --> 01:08:16,560
+في قانون ال الهيماتو كريت في القانون هذا هى اللى
+
+896
+01:08:16,560 --> 01:08:23,340
+هو 12 في 3 36 طبعا اذا الهيماتو كريت 36 او هو plus
+
+897
+01:08:23,340 --> 01:08:28,880
+minus 3فالمفروض إنه يقع داخل ال range ما بين تلاتة
+
+898
+01:08:28,880 --> 01:08:33,000
+و تلاتين لتسعة و تلاتين في المية طبعا لو بدي
+
+899
+01:08:33,000 --> 01:08:35,780
+أحولها letter per letter تلاتة و تلاتين من مية
+
+900
+01:08:35,780 --> 01:08:40,240
+لتسعة و تلاتين من مية letter per letter طبعا إذا
+
+901
+01:08:40,240 --> 01:08:44,720
+أحكي ستة و تلاتين في المية أو أحكي point ستة و
+
+902
+01:08:44,720 --> 01:08:48,620
+تلاتين من مية letter per letter نفس المعنىتمام؟
+
+903
+01:08:48,620 --> 01:08:52,620
+هذه طريقتين للتعبير عن قيمة ال hematocrit لذن هيك
+
+904
+01:08:52,620 --> 01:08:56,940
+بنكون وصلنا لنهاية محاضرتنا اليوم ان شاء الله
+
+905
+01:08:56,940 --> 01:09:01,640
+تكونوا استفدتوا واستمتعتوا في المحاضرة و اتمنى ان
+
+906
+01:09:01,640 --> 01:09:05,600
+يكون كل شيء واضح و لو ما كان في شيء واضح او عندكم
+
+907
+01:09:05,600 --> 01:09:10,140
+سؤال او استفسار ما تترددوا تسألوني اما على group
+
+908
+01:09:10,140 --> 01:09:14,380
+ال whats او ان شاء الله من خلال الاجتماع ال meet
+
+909
+01:09:14,380 --> 01:09:17,880
+ال google meet اللي احنا بنعقده اسبوعيايعطيكم
+
+910
+01:09:17,880 --> 01:09:20,240
+العافية وموثقين بإذن الله
+
diff --git a/PL9fwy3NUQKwY9jl8xw-bWkVZkWcGGyspd/KXPW3iBAdP0_raw.json b/PL9fwy3NUQKwY9jl8xw-bWkVZkWcGGyspd/KXPW3iBAdP0_raw.json
new file mode 100644
index 0000000000000000000000000000000000000000..d22cb0dc7a75a09d30d7dad85a5b472578502e17
--- /dev/null
+++ b/PL9fwy3NUQKwY9jl8xw-bWkVZkWcGGyspd/KXPW3iBAdP0_raw.json
@@ -0,0 +1 @@
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"word": " إيش", "probability": 0.8362630208333334}, {"start": 105.32, "end": 105.62, "word": " يعني", "probability": 0.945556640625}, {"start": 105.62, "end": 105.98, "word": " كلمة", "probability": 0.9879557291666666}, {"start": 105.98, "end": 106.34, "word": " micro", "probability": 0.970703125}, {"start": 106.34, "end": 106.98, "word": " hematocrit", "probability": 0.8919677734375}, {"start": 106.98, "end": 108.0, "word": " hematocrit", "probability": 0.74859619140625}, {"start": 108.0, "end": 108.68, "word": " لحالها", "probability": 0.98291015625}], "temperature": 1.0}, {"id": 6, "seek": 12894, "start": 109.5, "end": 128.94, "text": "هي عبارة عن نسبة ال RBCs بالنسبة لل whole blood أو ال volume اللي بتحتله ال RBCs، الحجم اللي بتحتله ال RBCs بالنسبة للحجم الكل لعينة الدم، تمام؟ النسبة المقاوية لل RBCs بالنسبة لل whole blood أو عينة ال whole blood", "tokens": [3224, 1829, 6225, 3555, 9640, 3660, 18871, 8717, 35457, 3660, 2423, 497, 7869, 82, 20666, 1863, 35457, 3660, 24976, 1379, 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"text": "و microhematocrit هو يعني إضافة كلمة microhematocrit ما هي إلا تعبير على إن أنا لما بشتغل الفحص هذا باخد small amount of sample", "tokens": [2407, 4532, 71, 8615, 905, 3210, 31439, 37495, 22653, 11933, 11242, 31845, 3660, 9122, 19528, 3660, 4532, 71, 8615, 905, 3210, 19446, 39896, 11933, 15040, 37279, 3555, 13546, 15844, 36145, 41850, 5296, 15042, 4724, 8592, 2655, 17082, 1211, 27188, 5016, 9381, 23758, 4724, 47283, 3215, 1359, 2372, 295, 6889], "avg_logprob": -0.17765625655651093, "compression_ratio": 1.2887323943661972, "no_speech_prob": 1.1920928955078125e-07, "words": [{"start": 129.51, "end": 129.81, "word": "و", "probability": 0.9453125}, {"start": 129.81, "end": 131.45, "word": " microhematocrit", "probability": 0.6720703125}, {"start": 131.45, "end": 131.93, "word": " هو", "probability": 0.389892578125}, {"start": 131.93, "end": 132.73, "word": " يعني", "probability": 0.911865234375}, {"start": 132.73, "end": 133.29, "word": " إضافة", "probability": 0.9766845703125}, 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376.43, "end": 377.55, "word": " المية،", "probability": 0.7308756510416666}, {"start": 377.55, "end": 377.71, "word": " اذا", "probability": 0.7252197265625}, {"start": 377.71, "end": 377.97, "word": " هو", "probability": 0.9619140625}, {"start": 377.97, "end": 378.59, "word": " بنعبر", "probability": 0.8741861979166666}, {"start": 378.59, "end": 378.95, "word": " عنه", "probability": 0.9833984375}, {"start": 378.95, "end": 379.25, "word": " as", "probability": 0.962890625}, {"start": 379.25, "end": 379.53, "word": " a", "probability": 0.9794921875}, {"start": 379.53, "end": 380.87, "word": " percentage،", "probability": 0.73193359375}, {"start": 380.87, "end": 381.77, "word": " تمام؟", "probability": 0.84814453125}, {"start": 381.77, "end": 381.99, "word": " اذا", "probability": 0.7626953125}, {"start": 381.99, "end": 382.55, "word": " بنعبر", "probability": 0.9529622395833334}, {"start": 382.55, "end": 382.99, "word": " عنه", "probability": 0.985107421875}, {"start": 382.99, "end": 383.33, "word": " as", "probability": 0.98291015625}, {"start": 383.33, "end": 383.57, "word": " a", "probability": 0.98828125}, {"start": 383.57, "end": 384.71, "word": " percentage،", "probability": 0.763671875}, {"start": 384.71, "end": 385.13, "word": " نسبة", "probability": 0.9892578125}, {"start": 385.13, "end": 385.77, "word": " مئوية،", "probability": 0.8517578125}, {"start": 385.77, "end": 385.99, "word": " كام", "probability": 0.822265625}, {"start": 385.99, "end": 386.51, "word": " تحتل", "probability": 0.8707682291666666}, {"start": 386.51, "end": 386.97, "word": " نسبة", "probability": 0.9773763020833334}, {"start": 386.97, "end": 387.21, "word": " ال", "probability": 0.9609375}, {"start": 387.21, "end": 387.21, "word": " ..", "probability": 0.6669921875}, {"start": 387.21, "end": 387.49, "word": " ال", "probability": 0.96630859375}, {"start": 387.49, "end": 388.13, "word": " RBCs", "probability": 0.6223958333333334}, {"start": 388.13, "end": 388.61, "word": " بالنسبة", "probability": 0.99560546875}, {"start": 388.61, "end": 388.77, "word": " لل", "probability": 0.88916015625}, {"start": 388.77, "end": 388.99, "word": " holy", "probability": 0.2459716796875}, {"start": 388.99, "end": 389.37, "word": " blood؟", "probability": 0.88916015625}, {"start": 389.37, "end": 389.51, "word": " انا", "probability": 0.75634765625}, {"start": 389.51, "end": 389.93, "word": " بعتبر", "probability": 0.8026529947916666}, {"start": 389.93, "end": 390.11, "word": " ان", "probability": 0.73681640625}, {"start": 390.11, "end": 390.73, "word": " العينة", "probability": 0.8583984375}, {"start": 390.73, "end": 391.11, "word": " كلها", "probability": 0.99658203125}, {"start": 391.11, "end": 391.29, "word": " اللي", "probability": 0.86181640625}, {"start": 391.29, "end": 391.43, "word": " انا", "probability": 0.7164306640625}, {"start": 391.43, "end": 392.09, "word": " جمعتها", "probability": 0.970703125}, {"start": 392.09, "end": 392.79, "word": " مية", "probability": 0.733154296875}, {"start": 392.79, "end": 393.61, "word": " بالمية،", "probability": 0.953125}, {"start": 393.61, "end": 393.85, "word": " كام", "probability": 0.9638671875}, {"start": 393.85, "end": 393.97, "word": " ال", "probability": 0.99560546875}, {"start": 393.97, "end": 394.61, "word": " RBCs", "probability": 0.9215494791666666}, {"start": 394.61, "end": 395.45, "word": " منها؟", "probability": 0.9934895833333334}], "temperature": 1.0}, {"id": 20, "seek": 41845, "start": 395.61, "end": 418.45, "text": "فرح نشوف كيف أنا ممكن أقيسها، تمام؟ إذا هذه هي فكرة الفحص، إذا ال principle مبدأ عمل الفحص أو فكرة الفحص أو يعني الافتراض أو نظرية الفحص، مبدأ عمل الفحص هو إن أنا ال hematocrite is usually determined by spinning a blood filled capillary tube in a centrifuge", "tokens": [5172, 2288, 5016, 8717, 8592, 38688, 9122, 33911, 41850, 3714, 43020, 5551, 38436, 3794, 11296, 12399, 46811, 10943, 22807, 11933, 15730, 29538, 39896, 6156, 4117, 25720, 27188, 5016, 9381, 12399, 11933, 15730, 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0.9879557291666666}, {"start": 410.71, "end": 411.21, "word": " هو", "probability": 0.857421875}, {"start": 411.21, "end": 411.53, "word": " إن", "probability": 0.69921875}, {"start": 411.53, "end": 411.95, "word": " أنا", "probability": 0.488037109375}, {"start": 411.95, "end": 412.29, "word": " ال", "probability": 0.95068359375}, {"start": 412.29, "end": 413.11, "word": " hematocrite", "probability": 0.62774658203125}, {"start": 413.11, "end": 413.35, "word": " is", "probability": 0.94580078125}, {"start": 413.35, "end": 413.95, "word": " usually", "probability": 0.91650390625}, {"start": 413.95, "end": 414.61, "word": " determined", "probability": 0.94775390625}, {"start": 414.61, "end": 415.17, "word": " by", "probability": 0.9833984375}, {"start": 415.17, "end": 415.73, "word": " spinning", "probability": 0.923828125}, {"start": 415.73, "end": 416.03, "word": " a", "probability": 0.94189453125}, {"start": 416.03, "end": 416.31, "word": " blood", "probability": 0.9208984375}, {"start": 416.31, "end": 416.63, "word": " filled", "probability": 0.59423828125}, {"start": 416.63, "end": 417.09, "word": " capillary", "probability": 0.968994140625}, {"start": 417.09, "end": 417.45, "word": " tube", "probability": 0.8955078125}, {"start": 417.45, "end": 417.63, "word": " in", "probability": 0.96630859375}, {"start": 417.63, "end": 417.79, "word": " a", "probability": 0.951171875}, {"start": 417.79, "end": 418.45, "word": " centrifuge", "probability": 0.941162109375}], "temperature": 1.0}, {"id": 21, "seek": 44660, "start": 420.34, "end": 446.6, "text": "بعمل determination، قياس، معرفة للـ hematocrit من خلال ان انا اعمل عملية spinning، centrifugation، separation، sedimentation، لاحظين المصطلحات دي كلها نفس المعنى لل blood في ال capillary blood في ال capillary، capillary ممتلقة بال blood من خلال ال centrifuge هاي capillary tube أنا عبقتها لتلت أربعها بال blood", "tokens": [3555, 25957, 1211, 18432, 12399, 12174, 25528, 3794, 12399, 20449, 28480, 3660, 24976, 39184, 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"word": " sedimentation،", "probability": 0.776611328125}, {"start": 432.98, "end": 433.3, "word": " لاحظين", "probability": 0.918701171875}, {"start": 433.3, "end": 433.92, "word": " المصطلحات", "probability": 0.9740397135416666}, {"start": 433.92, "end": 434.1, "word": " دي", "probability": 0.57830810546875}, {"start": 434.1, "end": 434.32, "word": " كلها", "probability": 0.979736328125}, {"start": 434.32, "end": 434.56, "word": " نفس", "probability": 0.99365234375}, {"start": 434.56, "end": 435.02, "word": " المعنى", "probability": 0.97998046875}, {"start": 435.02, "end": 435.22, "word": " لل", "probability": 0.6015625}, {"start": 435.22, "end": 435.66, "word": " blood", "probability": 0.83642578125}, {"start": 435.66, "end": 435.92, "word": " في", "probability": 0.927734375}, {"start": 435.92, "end": 436.02, "word": " ال", "probability": 0.87158203125}, {"start": 436.02, "end": 436.54, "word": " capillary", "probability": 0.807373046875}, {"start": 436.54, "end": 436.92, "word": " blood", "probability": 0.70166015625}, {"start": 436.92, "end": 437.16, "word": " في", "probability": 0.767578125}, {"start": 437.16, "end": 437.26, "word": " ال", "probability": 0.7587890625}, {"start": 437.26, "end": 438.18, "word": " capillary،", "probability": 0.770751953125}, {"start": 438.18, "end": 438.54, "word": " capillary", "probability": 0.824951171875}, {"start": 438.54, "end": 439.24, "word": " ممتلقة", "probability": 0.88466796875}, {"start": 439.24, "end": 439.48, "word": " بال", "probability": 0.97802734375}, {"start": 439.48, "end": 439.94, "word": " blood", "probability": 0.52099609375}, {"start": 439.94, "end": 440.38, "word": " من", "probability": 0.982421875}, {"start": 440.38, "end": 440.68, "word": " خلال", "probability": 0.994140625}, {"start": 440.68, "end": 440.8, "word": " ال", "probability": 0.9267578125}, {"start": 440.8, "end": 441.48, "word": " centrifuge", "probability": 0.900390625}, {"start": 441.48, "end": 442.14, "word": " هاي", "probability": 0.6138916015625}, {"start": 442.14, "end": 442.62, "word": " capillary", "probability": 0.913330078125}, {"start": 442.62, "end": 443.12, "word": " tube", "probability": 0.88037109375}, {"start": 443.12, "end": 444.04, "word": " أنا", "probability": 0.425537109375}, {"start": 444.04, "end": 444.82, "word": " عبقتها", "probability": 0.9329833984375}, {"start": 444.82, "end": 445.18, "word": " لتلت", "probability": 0.7042236328125}, {"start": 445.18, "end": 445.86, "word": " أربعها", "probability": 0.8717041015625}, {"start": 445.86, "end": 446.18, "word": " بال", "probability": 0.955078125}, {"start": 446.18, "end": 446.6, "word": " blood", "probability": 0.9453125}], "temperature": 1.0}, {"id": 22, "seek": 46949, "start": 446.71, "end": 469.49, "text": "سكرتها بيه لكليه أصبحت مغلقة أحد الأطراف مغلق و هذا before centrifuge after centrifuge راح يصير فيه packaging لال RBC's ال RBC's كلها راح قاش تتركز في منطقة واحدة اللي هي منطقة البطم يليها ال buffy coat و يليها ال plasma هاي ال plasma هذه كلها plasma تمام؟", "tokens": [3794, 37983, 2655, 11296, 4724, 1829, 3224, 5296, 4117, 20292, 3224, 5551, 9381, 3555, 33753, 3714, 17082, 1211, 28671, 5551, 24401, 16247, 9566, 23557, 5172, 3714, 17082, 1211, 4587, 4032, 23758, 949, 44828, 7181, 934, 44828, 7181, 12602, 39319, 7251, 9381, 13546, 8978, 3224, 16836, 5296, 6027, 497, 7869, 311, 2423, 497, 7869, 311, 28242, 11296, 12602, 39319, 12174, 33599, 6055, 2655, 31747, 11622, 8978, 9154, 9566, 28671, 36764, 24401, 3660, 13672, 1829, 39896, 9154, 9566, 28671, 29739, 9566, 2304, 7251, 20292, 11296, 2423, 9204, 88, 10690, 4032, 7251, 20292, 11296, 2423, 22564, 8032, 47302, 2423, 22564, 29538, 28242, 11296, 22564, 46811, 10943, 22807], "avg_logprob": -0.22157737754640125, "compression_ratio": 1.6371308016877637, "no_speech_prob": 8.940696716308594e-07, "words": [{"start": 446.71, "end": 447.75, "word": "سكرتها", "probability": 0.919677734375}, {"start": 447.75, "end": 448.09, "word": " بيه", "probability": 0.66259765625}, {"start": 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24, "seek": 50773, "start": 491.45, "end": 507.73, "text": "طبعا إذا أنا سحبت دم من الوريد وحطيته في edita tube في ال tube اللي اللونها lavender ال cap تبعها لونه mauve lavender طبعا هذا بيكون فيها edita anticoagulant طبعا فإذا أنا حطيته فيها", "tokens": [9566, 3555, 3615, 995, 11933, 15730, 41850, 8608, 5016, 3555, 2655, 11778, 2304, 9154, 2423, 13063, 25708, 4032, 5016, 9566, 36081, 3224, 8978, 1257, 2786, 9917, 8978, 2423, 9917, 13672, 1829, 13672, 11536, 11296, 43757, 2423, 1410, 6055, 3555, 3615, 11296, 5296, 11536, 3224, 22074, 303, 43757, 23032, 3555, 3615, 995, 23758, 4724, 1829, 30544, 8978, 11296, 1257, 2786, 2511, 2789, 559, 425, 394, 23032, 3555, 3615, 995, 6156, 28814, 15730, 41850, 11331, 9566, 36081, 3224, 8978, 11296], "avg_logprob": -0.22804588305799267, "compression_ratio": 1.6727272727272726, "no_speech_prob": 7.748603820800781e-06, "words": [{"start": 491.45, "end": 491.85, "word": "طبعا", "probability": 0.78955078125}, {"start": 491.85, "end": 492.35, "word": " 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لازم اكون عملت centrifugation ليش؟ لأنه اذا انا بدي اتأخر اكتر من هيك ممكن تتأثر يعني تتغير", "tokens": [27842, 10874, 2304, 3615, 18863, 9957, 3660, 5296, 31377, 2304, 1975, 8315, 1975, 30544, 1975, 25957, 1211, 44828, 697, 399, 5296, 31377, 2304, 1975, 30544, 6225, 42213, 2655, 44828, 697, 399, 32239, 8592, 22807, 5296, 33456, 3224, 1975, 15730, 1975, 8315, 4724, 16254, 1975, 2655, 10721, 34740, 1975, 4117, 2655, 2288, 9154, 39896, 4117, 3714, 43020, 6055, 2655, 10721, 49115, 37495, 22653, 6055, 2655, 17082, 13546], "avg_logprob": -0.15590018834640731, "compression_ratio": 1.527027027027027, "no_speech_prob": 0.0001239776611328125, "words": [{"start": 699.42, "end": 699.96, "word": "من", "probability": 0.9248046875}, {"start": 699.96, "end": 700.38, "word": " جمع", "probability": 0.9928385416666666}, {"start": 700.38, "end": 701.08, "word": " العينة", "probability": 0.8850911458333334}, {"start": 701.08, "end": 701.74, "word": " لازم", "probability": 0.9739583333333334}, {"start": 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إذن هيكون في عندي يعني فاقد أيضا في القراءة، في عندي نسبة قراءة ناقصة، يعني decreased، طبعا، في قراءة ال hematocrit", "tokens": [2304, 4117, 2407, 8315, 2655, 18863, 9957, 3660, 12399, 8978, 16490, 15040, 25528, 3714, 43020, 6055, 2655, 4117, 3794, 2288, 12399, 6055, 2655, 5016, 1211, 1211, 12399, 7251, 9381, 13546, 8978, 18871, 16254, 6156, 995, 28543, 8978, 33962, 15040, 25528, 12399, 36725, 8315, 5551, 9381, 15040, 12174, 2288, 16606, 31371, 39896, 6225, 3555, 9640, 3660, 18871, 8717, 35457, 3660, 16490, 15040, 25528, 12399, 11331, 7435, 2304, 13672, 1829, 39894, 33753, 1211, 33962, 15040, 25528, 20666, 1863, 35457, 3660, 24976, 10622, 3390, 12399, 6156, 28814, 15730, 20328, 9640, 8978, 18871, 16254, 6156, 995, 28543, 12399, 11933, 8848, 1863, 39896, 30544, 8978, 18871, 16254, 37495, 22653, 6156, 995, 28543, 36632, 11242, 995, 8978, 25062, 2288, 16606, 3660, 12399, 8978, 18871, 16254, 8717, 35457, 3660, 12174, 2288, 16606, 3660, 8717, 995, 4587, 9381, 3660, 12399, 37495, 22653, 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"probability": 0.93798828125}, {"start": 729.92, "end": 730.16, "word": " إذن", "probability": 0.641845703125}, {"start": 730.16, "end": 730.6, "word": " هيكون", "probability": 0.8720703125}, {"start": 730.6, "end": 730.74, "word": " في", "probability": 0.9765625}, {"start": 730.74, "end": 731.08, "word": " عندي", "probability": 0.975830078125}, {"start": 731.08, "end": 732.44, "word": " يعني", "probability": 0.749755859375}, {"start": 732.44, "end": 732.76, "word": " فاقد", "probability": 0.9895833333333334}, {"start": 732.76, "end": 733.08, "word": " أيضا", "probability": 0.93359375}, {"start": 733.08, "end": 733.2, "word": " في", "probability": 0.97607421875}, {"start": 733.2, "end": 733.88, "word": " القراءة،", "probability": 0.883740234375}, {"start": 733.88, "end": 733.98, "word": " في", "probability": 0.9208984375}, {"start": 733.98, "end": 734.68, "word": " عندي", "probability": 0.978759765625}, {"start": 734.68, "end": 736.08, "word": " نسبة", "probability": 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لإن ال hemolyse sample بيصير فيه فقد عندي في الخلايا اتكسرت خلايا ال RBCs طب أنا أصلا عم بحسب حجم ال RBCs حجم ال erythrocyte بالنسبة لل holy blood", "tokens": [39, 443, 16453, 405, 6889, 393, 380, 312, 1143, 337, 4997, 6225, 9957, 3660, 46811, 2655, 4386, 37495, 22653, 20328, 9640, 8978, 11296, 6055, 4117, 3794, 2288, 8978, 33962, 15040, 25528, 36632, 11242, 995, 37893, 2296, 37893, 10874, 25708, 3660, 5296, 33753, 7578, 41850, 5551, 8592, 2655, 17082, 1211, 8978, 11296, 8636, 267, 905, 3210, 983, 22807, 5296, 28814, 1863, 2423, 8636, 16453, 405, 6889, 4724, 1829, 9381, 13546, 8978, 3224, 6156, 28543, 18871, 16254, 8978, 33962, 15040, 25528, 1975, 2655, 4117, 3794, 43500, 16490, 15040, 25528, 2423, 497, 7869, 82, 23032, 3555, 41850, 5551, 9381, 15040, 6225, 2304, 4724, 5016, 35457, 11331, 7435, 2304, 2423, 497, 7869, 82, 11331, 7435, 2304, 2423, 1189, 88, 392, 340, 1344, 975, 20666, 1863, 35457, 3660, 24976, 10622, 3390], "avg_logprob": -0.1711368086769825, "compression_ratio": 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"probability": 0.98583984375}, {"start": 1678.9, "end": 1679.14, "word": " بدي", "probability": 0.91064453125}, {"start": 1679.14, "end": 1679.48, "word": " نسبة", "probability": 0.99560546875}, {"start": 1679.48, "end": 1679.66, "word": " ال", "probability": 0.98828125}, {"start": 1679.66, "end": 1679.68, "word": " ..", "probability": 0.68896484375}, {"start": 1679.68, "end": 1679.92, "word": " ال", "probability": 0.982421875}, {"start": 1679.92, "end": 1680.64, "word": " erythrocyte", "probability": 0.8008626302083334}, {"start": 1680.64, "end": 1680.94, "word": " فقط", "probability": 0.990234375}, {"start": 1680.94, "end": 1681.12, "word": " لا", "probability": 0.63671875}, {"start": 1681.12, "end": 1681.4, "word": " غير", "probability": 0.9912109375}], "temperature": 1.0}, {"id": 80, "seek": 170997, "start": 1682.47, "end": 1709.97, "text": "طيب يا بنات هذه ال reference ranges يعني ال normal range حكينا إنه ال normal value أنا بعبّر عنها ب range و ليس قيمة واحدة ل newborn إيش قيمة 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"word": " دراسات", "probability": 0.9920654296875}, {"start": 3003.01, "end": 3003.47, "word": " وجدوا", "probability": 0.9007161458333334}, {"start": 3003.47, "end": 3003.71, "word": " انه", "probability": 0.728271484375}, {"start": 3003.71, "end": 3004.07, "word": " افضل", "probability": 0.9315185546875}, {"start": 3004.07, "end": 3004.43, "word": " وقت", "probability": 0.9912109375}, {"start": 3004.43, "end": 3004.71, "word": " انه", "probability": 0.90869140625}, {"start": 3004.71, "end": 3004.99, "word": " ال", "probability": 0.8798828125}, {"start": 3004.99, "end": 3005.21, "word": " five", "probability": 0.78076171875}, {"start": 3005.21, "end": 3005.57, "word": " minutes", "probability": 0.94140625}, {"start": 3005.57, "end": 3006.23, "word": " في", "probability": 0.2235107421875}, {"start": 3006.23, "end": 3006.47, "word": " اعلى", "probability": 0.7994791666666666}, {"start": 3006.47, "end": 3006.81, "word": " سرعة", "probability": 0.9521484375}, {"start": 3006.81, "end": 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النتائج", "probability": 0.9639892578125}, {"start": 3131.11, "end": 3131.75, "word": " لإنه", "probability": 0.80908203125}, {"start": 3131.75, "end": 3131.87, "word": " في", "probability": 0.9150390625}, {"start": 3131.87, "end": 3132.11, "word": " هذا", "probability": 0.947265625}, {"start": 3132.11, "end": 3132.47, "word": " الوقت", "probability": 0.9811197916666666}, {"start": 3132.47, "end": 3132.75, "word": " ممكن", "probability": 0.985595703125}, {"start": 3132.75, "end": 3133.07, "word": " تكون", "probability": 0.97119140625}, {"start": 3133.07, "end": 3133.21, "word": " ال", "probability": 0.98388671875}, {"start": 3133.21, "end": 3134.13, "word": " RBCs", "probability": 0.7296549479166666}, {"start": 3134.13, "end": 3134.61, "word": " بدت", "probability": 0.8271484375}, {"start": 3134.61, "end": 3135.05, "word": " تحل", "probability": 0.7517903645833334}, {"start": 3135.05, "end": 3135.43, "word": " بدت", "probability": 0.831298828125}, {"start": 3135.43, "end": 3136.57, "word": " يعني", "probability": 0.796875}, {"start": 3136.57, "end": 3138.63, "word": " يبطل", "probability": 0.8837890625}, {"start": 3138.63, "end": 3140.47, "word": " ترسيب", "probability": 0.83046875}, {"start": 3140.47, "end": 3140.75, "word": " أو", "probability": 0.7216796875}, {"start": 3140.75, "end": 3141.29, "word": " يعني", "probability": 0.91357421875}, {"start": 3141.29, "end": 3142.97, "word": " sedimentation", "probability": 0.944580078125}], "temperature": 1.0}, {"id": 147, "seek": 315201, "start": 3143.19, "end": 3152.01, "text": "تمام فبالتالي ممكن انها تتحرك ايضا باتجاه البلازم وهذا ممكن يعملي falsely elevated hematocrit تمام", "tokens": [39237, 10943, 6156, 3555, 6027, 2655, 6027, 1829, 3714, 43020, 16472, 11296, 6055, 2655, 5016, 31747, 1975, 1829, 11242, 995, 4724, 9307, 7435, 40294, 29739, 1211, 31377, 2304, 37037, 15730, 3714, 43020, 7251, 25957, 20292, 16720, 736, 23457, 8636, 267, 905, 3210, 46811, 10943], "avg_logprob": -0.20885417328940498, "compression_ratio": 1.246031746031746, "no_speech_prob": 2.580881118774414e-05, "words": [{"start": 3143.19, "end": 3144.03, "word": "تمام", "probability": 0.739013671875}, {"start": 3144.03, "end": 3145.47, "word": " فبالتالي", "probability": 0.9136555989583334}, {"start": 3145.47, "end": 3145.87, "word": " ممكن", "probability": 0.922607421875}, {"start": 3145.87, "end": 3146.29, "word": " انها", "probability": 0.676513671875}, {"start": 3146.29, "end": 3146.77, "word": " تتحرك", "probability": 0.9842529296875}, {"start": 3146.77, "end": 3147.09, "word": " ايضا", "probability": 0.9437255859375}, {"start": 3147.09, "end": 3147.53, "word": " باتجاه", "probability": 0.910400390625}, {"start": 3147.53, "end": 3147.97, "word": " البلازم", "probability": 0.7789306640625}, {"start": 3147.97, "end": 3148.25, "word": " وهذا", "probability": 0.7314453125}, {"start": 3148.25, "end": 3148.55, "word": " ممكن", "probability": 0.856689453125}, {"start": 3148.55, "end": 3149.07, "word": " يعملي", "probability": 0.8328450520833334}, {"start": 3149.07, "end": 3149.99, "word": " falsely", "probability": 0.779296875}, {"start": 3149.99, "end": 3150.47, "word": " elevated", "probability": 0.93603515625}, {"start": 3150.47, "end": 3151.21, "word": " hematocrit", "probability": 0.787109375}, {"start": 3151.21, "end": 3152.01, "word": " تمام", "probability": 0.855712890625}], "temperature": 1.0}, {"id": 148, "seek": 317967, "start": 3155.89, "end": 3179.67, "text": "the buffy coat of a specimen shouldn't be included أنا مش لازم أقرأ و أضمن ال buffy coat من ضمن القراءة حكينا بقرأ من تحت ال .. ال .. ال buffy coat shouldn't be included with the reading because it's inclusion falsely elevated the result يعني القراءة تبعته لو أنا بدي أدخل ال buffy coat في القراءة تبعتي هذا هيرفعلي ال .. ال hematocrit وهذا خطأ", "tokens": [3322, 9204, 88, 10690, 295, 257, 34204, 4659, 380, 312, 5556, 41850, 37893, 5296, 31377, 2304, 5551, 4587, 2288, 10721, 4032, 5551, 11242, 27842, 2423, 9204, 88, 10690, 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"avg_logprob": -0.17624999418854714, "compression_ratio": 1.743718592964824, "no_speech_prob": 1.0728836059570312e-06, "words": [{"start": 3801.65, "end": 3802.15, "word": "طيب", "probability": 0.9833984375}, {"start": 3802.15, "end": 3802.79, "word": " أخر", "probability": 0.6824951171875}, {"start": 3802.79, "end": 3803.21, "word": " نقطة", "probability": 0.9889322916666666}, {"start": 3803.21, "end": 3803.35, "word": " في", "probability": 0.90478515625}, {"start": 3803.35, "end": 3804.03, "word": " المحاضرة", "probability": 0.97265625}, {"start": 3804.03, "end": 3804.27, "word": " اللي", "probability": 0.782470703125}, {"start": 3804.27, "end": 3804.47, "word": " هي", "probability": 0.85595703125}, {"start": 3804.47, "end": 3804.83, "word": " rule", "probability": 0.55517578125}, {"start": 3804.83, "end": 3805.03, "word": " of", "probability": 0.94384765625}, {"start": 3805.03, "end": 3805.37, "word": " three", "probability": 0.78662109375}, {"start": 3805.37, "end": 3806.37, "word": " قانون", "probability": 0.86962890625}, {"start": 3806.37, "end": 3807.47, "word": " التلاتة", "probability": 0.9384765625}, {"start": 3807.47, "end": 3808.37, "word": " بحكيلك", "probability": 0.9236328125}, {"start": 3808.37, "end": 3808.67, "word": " أنه", "probability": 0.746826171875}, {"start": 3808.67, "end": 3808.75, "word": " من", "probability": 0.84619140625}, {"start": 3808.75, "end": 3809.13, "word": " نسبة", "probability": 0.9913736979166666}, {"start": 3809.13, "end": 3809.23, "word": " ال", "probability": 0.99169921875}, {"start": 3809.23, "end": 3809.75, "word": " hematocrit", "probability": 0.70306396484375}, {"start": 3809.75, "end": 3809.97, "word": " اللي", "probability": 0.947265625}, {"start": 3809.97, "end": 3810.11, "word": " أنت", "probability": 0.6788330078125}, {"start": 3810.11, "end": 3810.95, "word": " بتحسبيها", "probability": 0.873828125}, {"start": 3810.95, "end": 3811.61, "word": " ممكن", "probability": 0.9873046875}, {"start": 3811.61, "end": 3812.13, "word": " تجيبي", "probability": 0.829248046875}, {"start": 3812.13, "end": 3812.27, "word": " ال", "probability": 0.86669921875}, {"start": 3812.27, "end": 3812.95, "word": " hemoglobin", "probability": 0.8859049479166666}, {"start": 3812.95, "end": 3814.37, "word": " تمام؟", "probability": 0.605712890625}, {"start": 3814.37, "end": 3814.97, "word": " تحسبي", "probability": 0.8592529296875}, {"start": 3814.97, "end": 3815.35, "word": " نسبة", "probability": 0.9947916666666666}, {"start": 3815.35, "end": 3815.45, "word": " ال", "probability": 0.99267578125}, {"start": 3815.45, "end": 3815.95, "word": " hemoglobin", "probability": 0.8779296875}, {"start": 3815.95, "end": 3816.39, "word": " يعني", "probability": 0.884033203125}, {"start": 3816.39, "end": 3817.61, "word": " قوة", "probability": 0.84912109375}, {"start": 3817.61, "end": 3818.05, "word": " الدم", "probability": 0.955078125}, {"start": 3818.05, "end": 3818.63, "word": " تمام؟", "probability": 0.7578125}, {"start": 3818.63, "end": 3819.07, "word": " بالبلد", "probability": 0.8323567708333334}, {"start": 3819.07, "end": 3820.19, "word": " من", "probability": 0.8671875}, {"start": 3820.19, "end": 3821.03, "word": " القرأة", "probability": 0.78277587890625}, {"start": 3821.03, "end": 3821.13, "word": " ال", "probability": 0.95849609375}, {"start": 3821.13, "end": 3821.69, "word": " hematocrit", "probability": 0.8189697265625}], "temperature": 1.0}, {"id": 179, "seek": 384621, "start": 3822.05, "end": 3846.21, "text": "من خلال تطبيق ال rule of three ولكن هاي القانون يا بنات أنا بس بطبقه عن الناس ال normal هو مابيكونش صالح ان اطبقه عن الناس ال abnormal و اللي عندها مشاكل يعني لو شخص normal طبيعي و كان مثلا ال hematocrit تبعه اتناش ال hematocrit تبعه ستة و تلاتين تمام؟ ستة و تلاتين", "tokens": [27842, 16490, 1211, 6027, 6055, 9566, 21292, 4587, 2423, 4978, 295, 1045, 35525, 19452, 8032, 47302, 25062, 7649, 11536, 35186, 4724, 8315, 2655, 41850, 4724, 3794, 4724, 9566, 3555, 4587, 3224, 18871, 2423, 8315, 3794, 2423, 2710, 31439, 3714, 16758, 1829, 30544, 8592, 20328, 6027, 5016, 16472, 1975, 9566, 3555, 4587, 3224, 18871, 2423, 8315, 3794, 2423, 32847, 4032, 13672, 1829, 43242, 11296, 37893, 995, 28820, 37495, 22653, 45164, 13412, 9778, 9381, 2710, 23032, 21292, 3615, 1829, 4032, 25961, 50113, 15040, 2423, 8636, 267, 3617, 270, 6055, 3555, 3615, 3224, 1975, 2655, 1863, 33599, 2423, 8636, 267, 3617, 270, 6055, 3555, 3615, 3224, 8608, 2655, 3660, 4032, 6055, 1211, 9307, 9957, 46811, 10943, 22807, 8608, 2655, 3660, 4032, 6055, 1211, 9307, 9957], "avg_logprob": -0.13211382622641277, "compression_ratio": 1.8933333333333333, "no_speech_prob": 1.1324882507324219e-06, "words": [{"start": 3822.05, "end": 3822.25, "word": "من", "probability": 0.99169921875}, {"start": 3822.25, "end": 3822.59, "word": " خلال", "probability": 0.9894205729166666}, {"start": 3822.59, "end": 3823.27, "word": " تطبيق", "probability": 0.9892578125}, {"start": 3823.27, "end": 3823.51, "word": " ال", "probability": 0.95263671875}, {"start": 3823.51, "end": 3823.77, "word": " rule", "probability": 0.85205078125}, {"start": 3823.77, "end": 3823.95, "word": " of", "probability": 0.9755859375}, {"start": 3823.95, "end": 3824.27, "word": " three", "probability": 0.78076171875}, {"start": 3824.27, "end": 3824.79, "word": " ولكن", "probability": 0.80859375}, {"start": 3824.79, "end": 3825.11, "word": " هاي", "probability": 0.7705078125}, {"start": 3825.11, "end": 3825.61, "word": " القانون", "probability": 0.9920247395833334}, {"start": 3825.61, "end": 3825.77, "word": " يا", "probability": 0.953125}, {"start": 3825.77, "end": 3826.25, "word": " بنات", "probability": 0.9186197916666666}, {"start": 3826.25, "end": 3826.71, "word": " أنا", "probability": 0.6083984375}, {"start": 3826.71, "end": 3826.93, "word": " بس", "probability": 0.9326171875}, {"start": 3826.93, "end": 3827.35, "word": " بطبقه", "probability": 0.86982421875}, {"start": 3827.35, "end": 3827.51, "word": " عن", "probability": 0.84912109375}, {"start": 3827.51, "end": 3827.71, "word": " الناس", "probability": 0.8052571614583334}, {"start": 3827.71, "end": 3827.85, "word": " ال", "probability": 0.9052734375}, {"start": 3827.85, "end": 3828.13, "word": " normal", "probability": 0.8857421875}, {"start": 3828.13, "end": 3828.41, "word": " هو", "probability": 0.8076171875}, {"start": 3828.41, "end": 3829.11, "word": " مابيكونش", "probability": 0.8125}, {"start": 3829.11, "end": 3829.59, "word": " صالح", "probability": 0.99609375}, {"start": 3829.59, "end": 3829.73, "word": " ان", "probability": 0.54248046875}, {"start": 3829.73, "end": 3830.11, "word": " اطبقه", "probability": 0.883203125}, {"start": 3830.11, "end": 3830.23, "word": " عن", "probability": 0.94873046875}, {"start": 3830.23, "end": 3830.41, "word": " الناس", "probability": 0.9630533854166666}, {"start": 3830.41, "end": 3830.55, "word": " ال", "probability": 0.9931640625}, {"start": 3830.55, "end": 3830.91, "word": " abnormal", "probability": 0.8916015625}, {"start": 3830.91, "end": 3831.13, "word": " و", "probability": 0.619140625}, {"start": 3831.13, "end": 3831.25, "word": " اللي", "probability": 0.80615234375}, {"start": 3831.25, "end": 3831.49, "word": " عندها", "probability": 0.993408203125}, {"start": 3831.49, "end": 3831.99, "word": " مشاكل", "probability": 0.9913736979166666}, {"start": 3831.99, "end": 3832.77, "word": " يعني", "probability": 0.878173828125}, {"start": 3832.77, "end": 3832.95, "word": " لو", "probability": 0.9794921875}, {"start": 3832.95, "end": 3833.29, "word": " شخص", "probability": 0.9983723958333334}, {"start": 3833.29, "end": 3833.71, "word": " normal", "probability": 0.91552734375}, {"start": 3833.71, "end": 3834.47, "word": " طبيعي", "probability": 0.9659423828125}, {"start": 3834.47, "end": 3834.97, "word": " و", "probability": 0.98974609375}, {"start": 3834.97, "end": 3835.21, "word": " كان", "probability": 0.85400390625}, {"start": 3835.21, "end": 3835.61, "word": " مثلا", "probability": 0.986083984375}, {"start": 3835.61, "end": 3835.77, "word": " ال", "probability": 0.994140625}, {"start": 3835.77, "end": 3836.37, "word": " hematocrit", "probability": 0.67236328125}, {"start": 3836.37, "end": 3837.49, "word": " تبعه", "probability": 0.9219970703125}, {"start": 3837.49, "end": 3840.39, "word": " اتناش", "probability": 0.8572998046875}, {"start": 3840.39, "end": 3841.15, "word": " ال", "probability": 0.47705078125}, {"start": 3841.15, "end": 3842.77, "word": " hematocrit", "probability": 0.912109375}, {"start": 3842.77, "end": 3843.33, "word": " تبعه", "probability": 0.97607421875}, {"start": 3843.33, "end": 3843.69, "word": " ستة", "probability": 0.9508463541666666}, {"start": 3843.69, "end": 3843.75, "word": " و", "probability": 0.794921875}, {"start": 3843.75, "end": 3844.19, "word": " تلاتين", "probability": 0.9656982421875}, {"start": 3844.19, "end": 3845.29, "word": " تمام؟", "probability": 0.583984375}, {"start": 3845.29, "end": 3845.67, "word": " ستة", "probability": 0.98583984375}, {"start": 3845.67, "end": 3845.77, "word": " و", "probability": 0.970703125}, {"start": 3845.77, "end": 3846.21, "word": " تلاتين", "probability": 0.9854736328125}], "temperature": 1.0}, {"id": 180, "seek": 387232, "start": 3847.92, "end": 3872.32, "text": "بحكيلك انه الهموجلبين هو ال hematocrit على تلاتة plus minus تلاتة طبعا خلينا انا اكتب القانون هنا الهموجلبين يا بنات يساوي ال hematocrit على تلاتة", "tokens": [49628, 4117, 1829, 23275, 16472, 3224, 2423, 16095, 29245, 1211, 21292, 1863, 31439, 2423, 8636, 267, 3617, 270, 15844, 6055, 1211, 9307, 3660, 1804, 3175, 6055, 1211, 9307, 3660, 23032, 3555, 3615, 995, 16490, 20292, 8315, 1975, 8315, 1975, 4117, 2655, 3555, 25062, 7649, 11536, 34105, 2423, 16095, 29245, 1211, 21292, 1863, 35186, 4724, 8315, 2655, 7251, 3794, 995, 45865, 2423, 8636, 267, 3617, 270, 15844, 6055, 1211, 9307, 3660], "avg_logprob": -0.22931338405944932, "compression_ratio": 1.6950354609929077, "no_speech_prob": 4.76837158203125e-07, "words": [{"start": 3847.92, "end": 3848.56, "word": "بحكيلك", "probability": 0.9293212890625}, {"start": 3848.56, "end": 3848.9, "word": " انه", "probability": 0.37451171875}, {"start": 3848.9, "end": 3849.78, "word": " الهموجلبين", "probability": 0.6527913411458334}, {"start": 3849.78, "end": 3850.42, "word": " هو", "probability": 0.98828125}, {"start": 3850.42, "end": 3850.72, "word": " ال", "probability": 0.99072265625}, {"start": 3850.72, "end": 3851.42, "word": " hematocrit", "probability": 0.56231689453125}, {"start": 3851.42, "end": 3851.94, "word": " على", "probability": 0.8115234375}, {"start": 3851.94, "end": 3852.56, "word": " تلاتة", "probability": 0.83868408203125}, {"start": 3852.56, "end": 3852.94, "word": " plus", "probability": 0.89404296875}, {"start": 3852.94, "end": 3853.34, "word": " minus", "probability": 0.9658203125}, {"start": 3853.34, "end": 3853.96, "word": " تلاتة", "probability": 0.96484375}, {"start": 3853.96, "end": 3854.94, "word": " طبعا", "probability": 0.764892578125}, {"start": 3854.94, "end": 3858.42, "word": " خلينا", "probability": 0.7091471354166666}, {"start": 3858.42, "end": 3858.68, "word": " انا", "probability": 0.686279296875}, {"start": 3858.68, "end": 3859.04, "word": " اكتب", "probability": 0.9625244140625}, {"start": 3859.04, "end": 3859.46, "word": " القانون", "probability": 0.98486328125}, {"start": 3859.46, "end": 3859.76, "word": " هنا", "probability": 0.98828125}, {"start": 3859.76, "end": 3861.32, "word": " الهموجلبين", "probability": 0.8889973958333334}, {"start": 3861.32, "end": 3861.74, "word": " يا", "probability": 0.953125}, {"start": 3861.74, "end": 3862.36, "word": " بنات", "probability": 0.94384765625}, {"start": 3862.36, "end": 3863.42, "word": " يساوي", "probability": 0.97412109375}, {"start": 3863.42, "end": 3864.8, "word": " ال", "probability": 0.49609375}, {"start": 3864.8, "end": 3866.48, "word": " hematocrit", "probability": 0.8470458984375}, {"start": 3866.48, "end": 3870.44, "word": " على", "probability": 0.489990234375}, {"start": 3870.44, "end": 3872.32, "word": " تلاتة", "probability": 0.9801025390625}], "temperature": 1.0}, {"id": 181, "seek": 390580, "start": 3878.48, "end": 3905.8, "text": "اللي هي ماتكرت على تلاتة plus minus plus minus يعني مش عارفة انا اطلعها من ال keyboard plus minus بس انتوا يعني هيها لحظة خليني أجيبها من هنا plus minus تلاتة", "tokens": [6027, 20292, 39896, 3714, 9307, 37983, 2655, 15844, 6055, 1211, 9307, 3660, 1804, 3175, 1804, 3175, 37495, 22653, 37893, 6225, 9640, 5172, 3660, 1975, 8315, 1975, 9566, 1211, 3615, 11296, 9154, 2423, 10186, 1804, 3175, 4724, 3794, 16472, 2655, 14407, 37495, 22653, 39896, 11296, 5296, 5016, 19913, 3660, 16490, 1211, 9957, 1829, 5551, 7435, 1829, 3555, 11296, 9154, 34105, 1804, 3175, 6055, 1211, 9307, 3660], "avg_logprob": -0.15980112959038129, "compression_ratio": 1.6026490066225165, "no_speech_prob": 4.76837158203125e-07, "words": [{"start": 3878.48, "end": 3878.7, "word": "اللي", "probability": 0.9658203125}, {"start": 3878.7, "end": 3878.8, "word": " هي", "probability": 0.438720703125}, {"start": 3878.8, "end": 3879.24, "word": " ماتكرت", "probability": 0.6343994140625}, {"start": 3879.24, "end": 3879.46, "word": " على", "probability": 0.8359375}, {"start": 3879.46, "end": 3880.2, "word": " تلاتة", "probability": 0.9033203125}, {"start": 3880.2, "end": 3881.34, "word": " plus", "probability": 0.6845703125}, {"start": 3881.34, "end": 3882.26, "word": " minus", "probability": 0.90966796875}, {"start": 3882.26, "end": 3886.5, "word": " plus", "probability": 0.8876953125}, {"start": 3886.5, "end": 3887.1, "word": " minus", "probability": 0.99560546875}, {"start": 3887.1, "end": 3887.76, "word": " يعني", "probability": 0.736572265625}, {"start": 3887.76, "end": 3890.24, "word": " مش", "probability": 0.70654296875}, {"start": 3890.24, "end": 3890.7, "word": " عارفة", "probability": 0.922119140625}, {"start": 3890.7, "end": 3890.82, "word": " انا", "probability": 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0.868896484375}, {"start": 3898.72, "end": 3899.06, "word": " أجيبها", "probability": 0.89072265625}, {"start": 3899.06, "end": 3899.2, "word": " من", "probability": 0.9970703125}, {"start": 3899.2, "end": 3899.5, "word": " هنا", "probability": 0.99609375}, {"start": 3899.5, "end": 3904.84, "word": " plus", "probability": 0.970703125}, {"start": 3904.84, "end": 3905.24, "word": " minus", "probability": 0.99365234375}, {"start": 3905.24, "end": 3905.8, "word": " تلاتة", "probability": 0.963623046875}], "temperature": 1.0}, {"id": 182, "seek": 393231, "start": 3906.61, "end": 3932.31, "text": "طبعا لو كان عندي الهيمتوكرت مثلا طلعته 36 أعطتكم مثال معناه الهيمتوكرت على تلاتة ستة و تلاتين على تلاتة اتناش طبعا اتناش plus minus تلاتة يعني ال homoglobin بدو يكون من اتناش ناقص تلاتة يعني من تسعة لاتناش plus تلاتة لخمستاش جرام per deciliter هذه واحدة قياس ال homoglobin", "tokens": [9566, 3555, 3615, 995, 45164, 25961, 18871, 16254, 2423, 3224, 32640, 2655, 2407, 37983, 2655, 50113, 15040, 23032, 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hemotocrit", "probability": 0.94775390625}], "temperature": 1.0}, {"id": 185, "seek": 400194, "start": 3984.72, "end": 4001.94, "text": "بعبر عنه بال percentage النسبة المقاوية و ممكن انا ما اعبر عنه بالنسبة المقاوية اعبر عنه بال letter تمام letter bear letter طيب letter bear letter", "tokens": [3555, 3615, 3555, 2288, 18871, 3224, 20666, 9668, 28239, 35457, 3660, 9673, 4587, 995, 2407, 10632, 4032, 3714, 43020, 1975, 8315, 19446, 1975, 3615, 3555, 2288, 18871, 3224, 20666, 1863, 35457, 3660, 9673, 4587, 995, 2407, 10632, 1975, 3615, 3555, 2288, 18871, 3224, 20666, 5063, 46811, 10943, 5063, 6155, 5063, 23032, 1829, 3555, 5063, 6155, 5063], "avg_logprob": -0.1839363972345988, "compression_ratio": 1.9043478260869566, "no_speech_prob": 2.086162567138672e-06, "words": [{"start": 3984.72, "end": 3985.42, "word": "بعبر", "probability": 0.796142578125}, {"start": 3985.42, "end": 3985.92, "word": " عنه", "probability": 0.9814453125}, {"start": 3985.92, "end": 3986.58, "word": " بال", 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اعبر", "probability": 0.8829345703125}, {"start": 3992.2, "end": 3992.72, "word": " عنه", "probability": 0.990234375}, {"start": 3992.72, "end": 3993.52, "word": " بال", "probability": 0.8310546875}, {"start": 3993.52, "end": 3994.32, "word": " letter", "probability": 0.6181640625}, {"start": 3994.32, "end": 3995.82, "word": " تمام", "probability": 0.7193603515625}, {"start": 3995.82, "end": 3996.82, "word": " letter", "probability": 0.440185546875}, {"start": 3996.82, "end": 3998.6, "word": " bear", "probability": 0.370361328125}, {"start": 3998.6, "end": 3999.54, "word": " letter", "probability": 0.72509765625}, {"start": 3999.54, "end": 4000.92, "word": " طيب", "probability": 0.9703776041666666}, {"start": 4000.92, "end": 4001.3, "word": " letter", "probability": 0.9306640625}, {"start": 4001.3, "end": 4001.58, "word": " bear", "probability": 0.896484375}, {"start": 4001.58, "end": 4001.94, "word": " letter", "probability": 0.96630859375}], "temperature": 1.0}, {"id": 186, "seek": 402095, "start": 4002.55, "end": 4020.95, "text": "يعني لو كان ال hematocrit 36 في المية كبدأ أحولها لوحدة letter per letter بقسمها عالميا ال 36 بدها تصيراش 36 من 100 letter per letter طبعا هذه بنات letter per letter بتروح مع بعضها يعني انا هيك بس حطاها decor", "tokens": [40228, 22653, 45164, 25961, 2423, 8636, 267, 905, 3210, 8652, 8978, 9673, 10632, 9122, 44510, 10721, 5551, 5016, 12610, 11296, 45164, 24401, 3660, 5063, 680, 5063, 4724, 4587, 38251, 11296, 6225, 45340, 25528, 2423, 8652, 47525, 11296, 6055, 9381, 13546, 33599, 8652, 9154, 2319, 5063, 680, 5063, 23032, 3555, 3615, 995, 29538, 4724, 8315, 2655, 5063, 680, 5063, 39894, 32887, 5016, 20449, 45030, 11242, 11296, 37495, 22653, 1975, 8315, 39896, 4117, 4724, 3794, 11331, 9566, 995, 11296, 7919], "avg_logprob": -0.20668513035472436, "compression_ratio": 1.641711229946524, "no_speech_prob": 1.3113021850585938e-06, "words": [{"start": 4002.55, "end": 4002.89, "word": "يعني", "probability": 0.9111328125}, {"start": 4002.89, 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"end": 4009.23, "word": " letter", "probability": 0.96923828125}, {"start": 4009.23, "end": 4010.19, "word": " بقسمها", "probability": 0.9256591796875}, {"start": 4010.19, "end": 4010.63, "word": " عالميا", "probability": 0.6704915364583334}, {"start": 4010.63, "end": 4010.75, "word": " ال", "probability": 0.71728515625}, {"start": 4010.75, "end": 4011.29, "word": " 36", "probability": 0.93115234375}, {"start": 4011.29, "end": 4011.55, "word": " بدها", "probability": 0.75439453125}, {"start": 4011.55, "end": 4012.31, "word": " تصيراش", "probability": 0.7501220703125}, {"start": 4012.31, "end": 4013.43, "word": " 36", "probability": 0.94970703125}, {"start": 4013.43, "end": 4013.81, "word": " من", "probability": 0.9697265625}, {"start": 4013.81, "end": 4014.21, "word": " 100", "probability": 0.71337890625}, {"start": 4014.21, "end": 4014.99, "word": " letter", "probability": 0.76416015625}, {"start": 4014.99, "end": 4015.87, "word": " per", "probability": 0.96630859375}, {"start": 4015.87, "end": 4016.47, "word": " letter", "probability": 0.94189453125}, {"start": 4016.47, "end": 4017.17, "word": " طبعا", "probability": 0.98779296875}, {"start": 4017.17, "end": 4017.35, "word": " هذه", "probability": 0.453857421875}, {"start": 4017.35, "end": 4017.67, "word": " بنات", "probability": 0.668212890625}, {"start": 4017.67, "end": 4017.87, "word": " letter", "probability": 0.93994140625}, {"start": 4017.87, "end": 4018.05, "word": " per", "probability": 0.81591796875}, {"start": 4018.05, "end": 4018.33, "word": " letter", "probability": 0.98583984375}, {"start": 4018.33, "end": 4018.65, "word": " بتروح", "probability": 0.97802734375}, {"start": 4018.65, "end": 4018.79, "word": " مع", "probability": 0.99755859375}, {"start": 4018.79, "end": 4019.31, "word": " بعضها", "probability": 0.99755859375}, {"start": 4019.31, "end": 4019.67, "word": " يعني", "probability": 0.94140625}, {"start": 4019.67, "end": 4019.87, "word": " انا", "probability": 0.7357177734375}, {"start": 4019.87, "end": 4020.05, "word": " هيك", "probability": 0.958740234375}, {"start": 4020.05, "end": 4020.19, "word": " بس", "probability": 0.97607421875}, {"start": 4020.19, "end": 4020.55, "word": " حطاها", "probability": 0.8331298828125}, {"start": 4020.55, "end": 4020.95, "word": " decor", "probability": 0.86328125}], "temperature": 1.0}, {"id": 187, "seek": 404206, "start": 4021.46, "end": 4042.06, "text": "طبعا و الفكرة يعني هو بيعبر عنها بال percentage بس انا ممكن يعني اعبر عنها بال letter per letter بس اقسم القيمة على 100 عشان تخلص من النسبة المئوية هذه طبعا اذا عشان لما تشوفوا قيمة ال hemotocrit ماعطيكم مياها بالقيمة هذه سهل جدا تحولوها لنسبة مئوية و تفهموش يعني الكلام هذا", "tokens": [9566, 3555, 3615, 995, 4032, 27188, 4117, 25720, 37495, 22653, 31439, 4724, 40228, 26890, 18871, 11296, 20666, 9668, 4724, 3794, 1975, 8315, 3714, 43020, 37495, 22653, 1975, 3615, 26890, 18871, 11296, 20666, 5063, 680, 5063, 4724, 3794, 1975, 4587, 38251, 25062, 32640, 3660, 15844, 2319, 6225, 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"end": 4024.5, "word": " هو", "probability": 0.7431640625}, {"start": 4024.5, "end": 4025.08, "word": " بيعبر", "probability": 0.5200602213541666}, {"start": 4025.08, "end": 4025.34, "word": " عنها", "probability": 0.994140625}, {"start": 4025.34, "end": 4025.5, "word": " بال", "probability": 0.96142578125}, {"start": 4025.5, "end": 4026.1, "word": " percentage", "probability": 0.90283203125}, {"start": 4026.1, "end": 4026.42, "word": " بس", "probability": 0.964599609375}, {"start": 4026.42, "end": 4026.56, "word": " انا", "probability": 0.6671142578125}, {"start": 4026.56, "end": 4026.86, "word": " ممكن", "probability": 0.9921875}, {"start": 4026.86, "end": 4027.08, "word": " يعني", "probability": 0.966796875}, {"start": 4027.08, "end": 4027.44, "word": " اعبر", "probability": 0.8175455729166666}, {"start": 4027.44, "end": 4027.7, "word": " عنها", "probability": 0.995849609375}, {"start": 4027.7, "end": 4027.86, "word": " بال", "probability": 0.85302734375}, {"start": 4027.86, "end": 4028.08, "word": " letter", "probability": 0.81005859375}, {"start": 4028.08, "end": 4028.28, "word": " per", "probability": 0.64794921875}, {"start": 4028.28, "end": 4028.6, "word": " letter", "probability": 0.943359375}, {"start": 4028.6, "end": 4029.12, "word": " بس", "probability": 0.989013671875}, {"start": 4029.12, "end": 4029.54, "word": " اقسم", "probability": 0.8600260416666666}, {"start": 4029.54, "end": 4030.04, "word": " القيمة", "probability": 0.9747721354166666}, {"start": 4030.04, "end": 4030.24, "word": " على", "probability": 0.6513671875}, {"start": 4030.24, "end": 4030.44, "word": " 100", "probability": 0.60791015625}, {"start": 4030.44, "end": 4030.66, "word": " عشان", "probability": 0.9869791666666666}, {"start": 4030.66, "end": 4031.08, "word": " تخلص", "probability": 0.9842529296875}, {"start": 4031.08, "end": 4031.24, "word": " من", "probability": 0.98193359375}, {"start": 4031.24, "end": 4031.62, "word": " النسبة", "probability": 0.9383138020833334}, {"start": 4031.62, "end": 4032.1, "word": " المئوية", "probability": 0.87451171875}, {"start": 4032.1, "end": 4032.52, "word": " هذه", "probability": 0.72509765625}, {"start": 4032.52, "end": 4033.42, "word": " طبعا", "probability": 0.9276123046875}, {"start": 4033.42, "end": 4033.98, "word": " اذا", "probability": 0.668701171875}, {"start": 4033.98, "end": 4034.36, "word": " عشان", "probability": 0.99072265625}, {"start": 4034.36, "end": 4034.54, "word": " لما", "probability": 0.974609375}, {"start": 4034.54, "end": 4034.9, "word": " تشوفوا", "probability": 0.9908447265625}, {"start": 4034.9, "end": 4035.42, "word": " قيمة", "probability": 0.9964192708333334}, {"start": 4035.42, "end": 4035.74, "word": " ال", "probability": 0.98583984375}, {"start": 4035.74, "end": 4036.28, "word": " hemotocrit", "probability": 0.6341552734375}, {"start": 4036.28, "end": 4036.78, "word": " ماعطيكم", "probability": 0.731640625}, {"start": 4036.78, "end": 4037.06, "word": " مياها", "probability": 0.6565755208333334}, {"start": 4037.06, "end": 4037.56, "word": " بالقيمة", "probability": 0.948486328125}, {"start": 4037.56, "end": 4037.92, "word": " هذه", "probability": 0.81640625}, {"start": 4037.92, "end": 4038.68, "word": " سهل", "probability": 0.974609375}, {"start": 4038.68, "end": 4038.92, "word": " جدا", "probability": 0.99462890625}, {"start": 4038.92, "end": 4039.42, "word": " تحولوها", "probability": 0.90830078125}, {"start": 4039.42, "end": 4039.86, "word": " لنسبة", "probability": 0.981689453125}, {"start": 4039.86, "end": 4040.34, "word": " مئوية", "probability": 0.96875}, {"start": 4040.34, "end": 4040.46, "word": " و", "probability": 0.6826171875}, {"start": 4040.46, "end": 4041.06, "word": " تفهموش", "probability": 0.84833984375}, {"start": 4041.06, "end": 4041.36, "word": " يعني", "probability": 0.76513671875}, {"start": 4041.36, "end": 4041.78, "word": " الكلام", "probability": 0.9837239583333334}, {"start": 4041.78, "end": 4042.06, "word": " هذا", "probability": 0.96044921875}], "temperature": 1.0}, {"id": 188, "seek": 407149, "start": 4042.57, "end": 4071.49, "text": "هي هنا معطيني انه when specimens are analyzed by either automated or manual method a quick visual check of the result of hemoglobin and hematocrit can be done لو انا اشتغلت ال hemoglobin او ال hematocrit بسواق automated او manual ممكن سهل جدا اطلع عليهم و اشوف هل هم a blind بيطبقوا ال rule of three بيطبقوا قانون التلاتة اللي هو اش بيحكي قانون التلاتة انه ال hemoglobin يساوي", "tokens": [3224, 1829, 34105, 20449, 9566, 9957, 1829, 16472, 3224, 562, 41007, 366, 28181, 538, 2139, 18473, 420, 9688, 3170, 257, 1702, 5056, 1520, 295, 264, 1874, 295, 8636, 45841, 13496, 293, 8636, 267, 905, 3210, 393, 312, 1096, 45164, 1975, 8315, 1975, 8592, 2655, 17082, 1211, 2655, 2423, 8636, 45841, 13496, 1975, 2407, 2423, 8636, 267, 905, 3210, 4724, 3794, 14407, 4587, 18473, 1975, 2407, 9688, 3714, 43020, 8608, 3224, 1211, 10874, 28259, 1975, 9566, 1211, 3615, 25894, 16095, 4032, 1975, 8592, 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4045.23, "end": 4045.61, "word": " are", "probability": 0.962890625}, {"start": 4045.61, "end": 4046.29, "word": " analyzed", "probability": 0.8134765625}, {"start": 4046.29, "end": 4046.99, "word": " by", "probability": 0.9609375}, {"start": 4046.99, "end": 4047.51, "word": " either", "probability": 0.951171875}, {"start": 4047.51, "end": 4048.09, "word": " automated", "probability": 0.9521484375}, {"start": 4048.09, "end": 4048.47, "word": " or", "probability": 0.890625}, {"start": 4048.47, "end": 4048.89, "word": " manual", "probability": 0.97119140625}, {"start": 4048.89, "end": 4049.47, "word": " method", "probability": 0.93896484375}, {"start": 4049.47, "end": 4049.91, "word": " a", "probability": 0.59375}, {"start": 4049.91, "end": 4050.19, "word": " quick", "probability": 0.9501953125}, {"start": 4050.19, "end": 4050.65, "word": " visual", "probability": 0.9072265625}, {"start": 4050.65, "end": 4051.07, "word": " check", "probability": 0.93505859375}, {"start": 4051.07, "end": 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"word": " في", "probability": 0.95947265625}, {"start": 4121.48, "end": 4121.76, "word": " المية", "probability": 0.99267578125}, {"start": 4121.76, "end": 4121.96, "word": " أو", "probability": 0.73583984375}, {"start": 4121.96, "end": 4122.88, "word": " أحكي", "probability": 0.926025390625}, {"start": 4122.88, "end": 4123.92, "word": " point", "probability": 0.962890625}, {"start": 4123.92, "end": 4124.66, "word": " ستة", "probability": 0.8932291666666666}, {"start": 4124.66, "end": 4124.72, "word": " و", "probability": 0.99755859375}, {"start": 4124.72, "end": 4125.04, "word": " تلاتين", "probability": 0.9862060546875}, {"start": 4125.04, "end": 4125.22, "word": " من", "probability": 0.99658203125}, {"start": 4125.22, "end": 4125.42, "word": " مية", "probability": 0.964111328125}, {"start": 4125.42, "end": 4125.72, "word": " letter", "probability": 0.90966796875}, {"start": 4125.72, "end": 4125.96, "word": " per", "probability": 0.96728515625}, {"start": 4125.96, "end": 4126.28, "word": " letter", "probability": 0.9658203125}, {"start": 4126.28, "end": 4126.6, "word": " نفس", "probability": 0.980224609375}, {"start": 4126.6, "end": 4127.14, "word": " المعنى", "probability": 0.988037109375}], "temperature": 1.0}, {"id": 192, "seek": 415684, "start": 4127.5, "end": 4156.84, "text": "تمام؟ هذه طريقتين للتعبير عن قيمة ال hematocrit لذن هيك بنكون وصلنا لنهاية محاضرتنا اليوم ان شاء الله تكونوا استفدتوا واستمتعتوا في المحاضرة و اتمنى ان يكون كل شيء واضح و لو ما كان في شيء واضح او عندكم سؤال او استفسار ما تترددوا تسألوني اما على group ال whats او ان شاء الله من خلال الاجتماع ال meet ال google meet اللي احنا بنعقده اسبوعيا", "tokens": [39237, 10943, 22807, 29538, 23032, 16572, 38149, 9957, 24976, 2655, 3615, 3555, 13546, 18871, 12174, 32640, 3660, 2423, 8636, 267, 905, 3210, 5296, 8848, 1863, 39896, 4117, 44945, 30544, 4032, 36520, 8315, 5296, 1863, 11296, 10632, 3714, 5016, 46958, 43500, 8315, 45595, 20498, 16472, 13412, 16606, 21984, 6055, 30544, 14407, 44713, 5172, 3215, 2655, 14407, 36764, 14851, 2304, 2655, 34268, 14407, 8978, 9673, 5016, 46958, 25720, 4032, 1975, 2655, 27842, 7578, 16472, 7251, 30544, 28242, 44049, 38207, 4032, 46958, 5016, 4032, 45164, 19446, 25961, 8978, 44049, 38207, 4032, 46958, 5016, 1975, 2407, 43242, 24793, 8608, 33604, 6027, 1975, 2407, 44713, 36178, 9640, 19446, 6055, 2655, 2288, 3215, 3215, 14407, 6055, 3794, 10721, 1211, 11536, 1829, 1975, 15042, 15844, 1594, 2423, 29625, 1975, 2407, 16472, 13412, 16606, 21984, 9154, 16490, 1211, 6027, 2423, 26108, 2655, 15042, 3615, 2423, 1677, 2423, 20742, 1677, 13672, 1829, 1975, 5016, 8315, 44945, 3615, 28543, 3224, 24525, 3555, 45367, 25528], "avg_logprob": -0.1252016077118535, "compression_ratio": 1.867741935483871, "no_speech_prob": 2.872943878173828e-05, "words": [{"start": 4127.5, "end": 4128.62, "word": "تمام؟", "probability": 0.85205078125}, {"start": 4128.62, "end": 4128.88, "word": " هذه", "probability": 0.69775390625}, {"start": 4128.88, "end": 4129.42, "word": " طريقتين", "probability": 0.9754638671875}, {"start": 4129.42, "end": 4130.0, "word": " للتعبير", "probability": 0.951953125}, {"start": 4130.0, "end": 4130.24, "word": " عن", "probability": 0.99365234375}, {"start": 4130.24, "end": 4130.72, "word": " قيمة", "probability": 0.9972330729166666}, {"start": 4130.72, "end": 4130.86, "word": " ال", "probability": 0.96875}, {"start": 4130.86, "end": 4131.42, "word": " hematocrit", "probability": 0.6531982421875}, {"start": 4131.42, "end": 4132.28, "word": " لذن", "probability": 0.4383544921875}, {"start": 4132.28, "end": 4132.62, "word": " هيك", "probability": 0.96142578125}, {"start": 4132.62, "end": 4133.24, "word": " بنكون", "probability": 0.9716796875}, {"start": 4133.24, "end": 4134.2, "word": " وصلنا", "probability": 0.9837239583333334}, {"start": 4134.2, "end": 4135.08, "word": " لنهاية", "probability": 0.962890625}, {"start": 4135.08, "end": 4135.84, "word": " محاضرتنا", "probability": 0.9572265625}, {"start": 4135.84, "end": 4136.32, "word": " اليوم", "probability": 0.807861328125}, {"start": 4136.32, "end": 4136.76, "word": " ان", "probability": 0.8212890625}, {"start": 4136.76, "end": 4136.9, "word": " شاء", "probability": 0.97021484375}, {"start": 4136.9, "end": 4136.94, "word": " الله", "probability": 0.94677734375}, {"start": 4136.94, "end": 4137.36, "word": " تكونوا", "probability": 0.9903971354166666}, {"start": 4137.36, "end": 4138.04, "word": " استفدتوا", "probability": 0.8978515625}, {"start": 4138.04, "end": 4139.4, "word": " واستمتعتوا", "probability": 0.8928629557291666}, {"start": 4139.4, "end": 4139.66, "word": " في", "probability": 0.9169921875}, {"start": 4139.66, "end": 4140.5, "word": " المحاضرة", "probability": 0.9951171875}, {"start": 4140.5, "end": 4140.92, "word": " و", "probability": 0.71337890625}, {"start": 4140.92, "end": 4141.5, "word": " اتمنى", "probability": 0.734619140625}, {"start": 4141.5, "end": 4141.64, "word": " ان", "probability": 0.76220703125}, {"start": 4141.64, "end": 4141.94, "word": " يكون", "probability": 0.943115234375}, {"start": 4141.94, "end": 4142.14, "word": " كل", "probability": 0.98779296875}, {"start": 4142.14, "end": 4142.44, "word": " شيء", "probability": 0.625244140625}, {"start": 4142.44, "end": 4142.82, "word": " واضح", "probability": 0.94970703125}, {"start": 4142.82, "end": 4143.0, "word": " و", "probability": 0.8701171875}, {"start": 4143.0, "end": 4143.3, "word": " لو", "probability": 0.96484375}, {"start": 4143.3, "end": 4143.56, "word": " ما", "probability": 0.97021484375}, {"start": 4143.56, "end": 4143.9, "word": " كان", "probability": 0.66357421875}, {"start": 4143.9, "end": 4144.08, "word": " في", "probability": 0.89306640625}, {"start": 4144.08, "end": 4144.4, "word": " شيء", "probability": 0.850341796875}, {"start": 4144.4, "end": 4144.82, "word": " واضح", "probability": 0.9724934895833334}, {"start": 4144.82, "end": 4145.2, "word": " او", "probability": 0.787353515625}, {"start": 4145.2, "end": 4145.6, "word": " عندكم", "probability": 0.976806640625}, {"start": 4145.6, "end": 4146.08, "word": " سؤال", "probability": 0.99755859375}, {"start": 4146.08, "end": 4146.34, "word": " او", "probability": 0.921875}, {"start": 4146.34, "end": 4146.92, "word": " استفسار", "probability": 0.98095703125}, {"start": 4146.92, "end": 4147.36, "word": " ما", "probability": 0.8603515625}, {"start": 4147.36, "end": 4147.96, "word": " تترددوا", "probability": 0.9874674479166666}, {"start": 4147.96, "end": 4148.92, "word": " تسألوني", "probability": 0.944091796875}, {"start": 4148.92, "end": 4149.66, "word": " اما", "probability": 0.90478515625}, {"start": 4149.66, "end": 4149.92, "word": " على", "probability": 0.875}, {"start": 4149.92, "end": 4150.14, "word": " group", "probability": 0.87744140625}, {"start": 4150.14, "end": 4150.32, "word": " ال", "probability": 0.97314453125}, {"start": 4150.32, "end": 4150.64, "word": " whats", "probability": 0.391845703125}, {"start": 4150.64, "end": 4151.44, "word": " او", "probability": 0.762939453125}, {"start": 4151.44, "end": 4152.18, "word": " ان", "probability": 0.931640625}, {"start": 4152.18, "end": 4152.32, "word": " شاء", "probability": 0.99267578125}, {"start": 4152.32, "end": 4152.54, "word": " الله", "probability": 0.9521484375}, {"start": 4152.54, "end": 4152.78, "word": " من", "probability": 0.99462890625}, {"start": 4152.78, "end": 4153.22, "word": " خلال", "probability": 0.99560546875}, {"start": 4153.22, "end": 4153.98, "word": " الاجتماع", "probability": 0.930078125}, {"start": 4153.98, "end": 4154.14, "word": " ال", "probability": 0.89697265625}, {"start": 4154.14, "end": 4154.38, "word": " meet", "probability": 0.896484375}, {"start": 4154.38, "end": 4154.72, "word": " ال", "probability": 0.86083984375}, {"start": 4154.72, "end": 4155.08, "word": " google", "probability": 0.68994140625}, {"start": 4155.08, "end": 4155.36, "word": " meet", "probability": 0.97998046875}, {"start": 4155.36, "end": 4155.5, "word": " اللي", "probability": 0.934326171875}, {"start": 4155.5, "end": 4155.68, "word": " احنا", "probability": 0.93505859375}, {"start": 4155.68, "end": 4156.24, "word": " بنعقده", "probability": 0.9681396484375}, {"start": 4156.24, "end": 4156.84, "word": " اسبوعيا", "probability": 0.856689453125}], "temperature": 1.0}, {"id": 193, "seek": 416024, "start": 4157.36, "end": 4160.24, "text": "يعطيكم العافية وموثقين بإذن الله", "tokens": [40228, 9566, 1829, 24793, 18863, 31845, 10632, 4032, 2304, 2407, 12984, 4587, 9957, 4724, 28814, 8848, 1863, 21984], "avg_logprob": -0.056589228150091674, "compression_ratio": 0.9523809523809523, "no_speech_prob": 0.0, "words": [{"start": 4157.36, "end": 4157.88, "word": "يعطيكم", "probability": 0.902099609375}, {"start": 4157.88, "end": 4158.62, "word": " العافية", "probability": 0.9724934895833334}, {"start": 4158.62, "end": 4159.64, "word": " وموثقين", "probability": 0.9439290364583334}, {"start": 4159.64, "end": 4160.02, "word": " بإذن", "probability": 0.980224609375}, {"start": 4160.02, "end": 4160.24, "word": " الله", "probability": 0.95654296875}], "temperature": 1.0}], "language": "ar", "language_probability": 1.0, "duration": 4161.6195, "duration_after_vad": 4094.794374999991}
\ No newline at end of file
diff --git a/PL9fwy3NUQKwY9jl8xw-bWkVZkWcGGyspd/KXPW3iBAdP0_raw.srt b/PL9fwy3NUQKwY9jl8xw-bWkVZkWcGGyspd/KXPW3iBAdP0_raw.srt
new file mode 100644
index 0000000000000000000000000000000000000000..0f90c9919cb114a75ea0cafb35d1ab0bddcd41d1
--- /dev/null
+++ b/PL9fwy3NUQKwY9jl8xw-bWkVZkWcGGyspd/KXPW3iBAdP0_raw.srt
@@ -0,0 +1,3640 @@
+1
+00:00:05,900 --> 00:00:09,780
+السلام عليكم ورحمة الله وبركاته طالبات العزيزات ان
+
+2
+00:00:09,780 --> 00:00:13,380
+شاء الله اليوم رح نكون مع بعض بمحاضرة عملية بعنوان
+
+3
+00:00:13,380 --> 00:00:19,080
+micro-hematocrat طبعا micro-hematocrat ولكن قبل ما
+
+4
+00:00:19,080 --> 00:00:24,220
+نبدأ بمحاضرة micro-hematocrat خلونا نسترجع مع بعض
+
+5
+00:00:24,220 --> 00:00:28,060
+احنا ايش أخدنا بالمحاضرة النظرية السابقةتحدثنا عن
+
+6
+00:00:28,060 --> 00:00:32,280
+جهاز ال centrifuge حكينا عن المكونات جهاز ال
+
+7
+00:00:32,280 --> 00:00:36,140
+centrifuge حكينا أيضا على أنواع جهاز ال centrifuge
+
+8
+00:00:36,140 --> 00:00:40,200
+على مبدأ عمله و قوة الترد المركزي اللي من خلالها
+
+9
+00:00:40,200 --> 00:00:45,640
+بيتنفصل العينات و بحيث انه بتترسب الأجزاء الثقيلة
+
+10
+00:00:45,640 --> 00:00:49,520
+او المكونات الثقيلة في العينة في ال bottom of tube
+
+11
+00:00:49,520 --> 00:00:53,650
+و الأجزاء الخفيفة بتكونat surface او at that top
+
+12
+00:00:53,650 --> 00:01:00,030
+تمام؟ و ايضا تحدثنا عن التعليمات الهامة للاستخدام
+
+13
+00:01:00,030 --> 00:01:03,910
+instant refuse حكينا انه لازم انا اتبع ال
+
+14
+00:01:03,910 --> 00:01:07,290
+manufacturer recommendation اللي هي توصيات الجهة
+
+15
+00:01:07,290 --> 00:01:12,430
+المصنعة و انه انا ايضا لازم اخد بعين الاعتباربعض
+
+16
+00:01:12,430 --> 00:01:17,410
+التعليمات زي مثلا أن أحط الجهاز على بنش ثابت أسكّف
+
+17
+00:01:17,410 --> 00:01:21,490
+الجهاز قبل ما أنا أشغله ممنوع أوقف الجهاز إيقاف
+
+18
+00:01:21,490 --> 00:01:24,470
+مفاجئ أو أحاول أن أنا أبطئ سرعته بإيدي أثناء
+
+19
+00:01:24,470 --> 00:01:29,470
+إيقافه وغيرها من التعليمات اللي يجب مراعتها ويجب
+
+20
+00:01:29,470 --> 00:01:34,130
+اتباعها أثناء العمل أو تشغيل جهاز ال centrifuge
+
+21
+00:01:34,680 --> 00:01:40,120
+كتوظيف لمعرفتنا حول جهاز ال centrifuge رح ناخد
+
+22
+00:01:40,120 --> 00:01:44,220
+تطبيق عليه وهو فحص ال micro hematocrit خلونا
+
+23
+00:01:44,220 --> 00:01:46,980
+بالأول نشوف إيش يعني كلمة micro hematocrit
+
+24
+00:01:46,980 --> 00:01:54,280
+hematocrit لحالهاهي عبارة عن نسبة ال RBCs بالنسبة
+
+25
+00:01:54,280 --> 00:01:58,380
+لل whole blood أو ال volume اللي بتحتله ال RBCs،
+
+26
+00:01:58,380 --> 00:02:02,080
+الحجم اللي بتحتله ال RBCs بالنسبة للحجم الكل لعينة
+
+27
+00:02:02,080 --> 00:02:07,040
+الدم، تمام؟ النسبة المقاوية لل RBCs بالنسبة لل
+
+28
+00:02:07,040 --> 00:02:09,810
+whole blood أو عينة ال whole bloodو
+
+29
+00:02:09,810 --> 00:02:14,650
+microhematocrit هو يعني إضافة كلمة microhematocrit
+
+30
+00:02:14,650 --> 00:02:20,210
+ما هي إلا تعبير على إن أنا لما بشتغل الفحص هذا
+
+31
+00:02:20,210 --> 00:02:27,600
+باخد small amount of samplemicro sample من خلال ال
+
+32
+00:02:27,600 --> 00:02:32,560
+skin puncture طبعا من خلال ال skin puncture بشتغل
+
+33
+00:02:32,560 --> 00:02:35,140
+طبعا احنا حكينا micro sampling او ال skin puncture
+
+34
+00:02:35,140 --> 00:02:40,660
+نفس المعنى فإذا ال micro sampling انه انا باخد
+
+35
+00:02:40,660 --> 00:02:46,320
+عينة حجمها صغير في tube دقيق جدا رفيع جدا micro
+
+36
+00:02:46,320 --> 00:02:50,680
+tubeطبعا عشان هيك صار اسمه micro hematocrit طبعا
+
+37
+00:02:50,680 --> 00:02:55,000
+إذا إضافة micro فقط على التعبير عن حجم العينة وإنه
+
+38
+00:02:55,000 --> 00:02:58,340
+أنا باخد كمية قليلة من ال blood لحتى أشتغل هذا
+
+39
+00:02:58,340 --> 00:03:02,320
+الفحص micro hematocrit إله اسم آخر أو ال
+
+40
+00:03:02,320 --> 00:03:06,640
+hematocrit إله اسم آخرbacked cell volume of holy
+
+41
+00:03:06,640 --> 00:03:10,020
+blood إيش يعني backed cell volume؟ يعني أنا بدي
+
+42
+00:03:10,020 --> 00:03:15,720
+أخد حجم اللي بتحتله ال RBCs الخلايا أثناء بعد ما
+
+43
+00:03:15,720 --> 00:03:18,900
+يصيرلها packaging أو backed بعد ما يصير إيش صارلها
+
+44
+00:03:18,900 --> 00:03:22,180
+packaging يعني بعد ما صارلهاsedimentation أو
+
+45
+00:03:22,180 --> 00:03:25,480
+spinning أو centrifugation أو separation أنا يعني
+
+46
+00:03:25,480 --> 00:03:31,180
+جمعت ال RBCs لمّتها على بعضها يعني رسّبتها أو يعني
+
+47
+00:03:31,180 --> 00:03:36,940
+حصرتها في volume معين و بدي أشوف نسبة ال volume
+
+48
+00:03:36,940 --> 00:03:40,960
+هذا كام بتحتال نسبة ال RBCs بالنسبة لل holy blood
+
+49
+00:03:40,960 --> 00:03:44,880
+بالنسبة لحجم العينة كلهاإذن, hematocrite is
+
+50
+00:03:44,880 --> 00:03:49,900
+defined as the volume occupied by erythrocyte in a
+
+51
+00:03:49,900 --> 00:03:54,180
+given volume of blood and is usually expressed as
+
+52
+00:03:54,180 --> 00:03:56,780
+a percentage of the volume of the whole blood
+
+53
+00:03:56,780 --> 00:04:01,240
+sample. The hematocrite may also be referred to as
+
+54
+00:04:01,240 --> 00:04:05,660
+bagged cell volumeIran الهيماتوكره بيعرف على انه
+
+55
+00:04:05,660 --> 00:04:10,440
+ايش؟ ال volume occupied الحجم اللي بتحتله مين ال
+
+56
+00:04:10,440 --> 00:04:15,780
+RBCs فقط ال RBCs تمام؟ و طبعا بالنسبة لإيش؟
+
+57
+00:04:15,780 --> 00:04:19,100
+بالنسبة ل a given volume of blood بالنسبة للحجم
+
+58
+00:04:19,100 --> 00:04:24,700
+اللي هو ال total blood او ال whole bloodتمام؟ و ال
+
+59
+00:04:24,700 --> 00:04:28,560
+hematocrit بنسميه backs of volume و عرفنا إيش يعني
+
+60
+00:04:28,560 --> 00:04:32,780
+كلمة أو الترجمة الحرفية لbacks of volume فباختصار
+
+61
+00:04:32,780 --> 00:04:37,060
+شديد احنا اليوم راح نشتغل أو يعني راح نتعلم كتب و
+
+62
+00:04:37,060 --> 00:04:40,820
+بنشغل ال micro hematocrit من خلال أنه أنا أجيب
+
+63
+00:04:40,820 --> 00:04:45,000
+tube رفيعة جدا دقيقة جدا بسميها capillary tube
+
+64
+00:04:45,000 --> 00:04:49,420
+طبعا هاي ال capillary tube بتكون مفتوحة الطرفينو
+
+65
+00:04:49,420 --> 00:04:54,000
+انا ب .. ب .. ب .. من خلال skin puncture احداث
+
+66
+00:04:54,000 --> 00:04:57,380
+puncture في ال skin او من خلال ان انا اغمسها في
+
+67
+00:04:57,380 --> 00:05:01,780
+tube في blood فال .. الدم راح يطلع في هاي ال blood
+
+68
+00:05:01,780 --> 00:05:05,200
+او راح يملأ هاي ال capillary ال blood راح يطلع
+
+69
+00:05:05,200 --> 00:05:08,940
+يملأ هاي ال capillary بالخاصية الشعرية عشان هيك
+
+70
+00:05:08,940 --> 00:05:12,300
+بسميها capillary tube طبعا بال capillary action
+
+71
+00:05:12,300 --> 00:05:16,440
+بيملأ ال tube بالخاصية الشعرية ال capillary action
+
+72
+00:05:16,440 --> 00:05:20,250
+عشان هيك ال tube اسمها capillary tubeطبعا طيب بعد
+
+73
+00:05:20,250 --> 00:05:23,990
+ما أنا أملأ اليوتيوب لحتى تقريبا تلت أرباعها رح
+
+74
+00:05:23,990 --> 00:05:28,950
+أسكر طرف من أطرافها باستخدام clay اش يعني clay؟
+
+75
+00:05:28,950 --> 00:05:32,290
+معجونة أو طينة حرة المعجونة اللي بيلعبوا فيها
+
+76
+00:05:32,290 --> 00:05:34,930
+الأطفال هي اللي بتكون في ألعاب الأطفال هي اللي
+
+77
+00:05:34,930 --> 00:05:40,020
+باستخدمها طبعاطيب أصبح ليتيوب صارت مغلقة الطرفين
+
+78
+00:05:40,020 --> 00:05:43,980
+لو أنا حاطيتها في جهاز centrifuge خاص فيها بسميه
+
+79
+00:05:43,980 --> 00:05:48,860
+micro hematocrit centrifuge تمام؟ فراح يفصللياها
+
+80
+00:05:48,860 --> 00:05:53,340
+بحيث أنه الأجزاء الثقيلة راح تكون at the bottom
+
+81
+00:05:54,260 --> 00:05:57,440
+والأجزاء الخفيفة زي ال plasma هتكون at the top
+
+82
+00:05:57,440 --> 00:06:04,000
+فانا بحسب كام .. او يعني كام تحتل ال RBC بالنسبة
+
+83
+00:06:04,000 --> 00:06:07,760
+لل whole blood وبيطلع عندي قراقة ال hematocrit طب
+
+84
+00:06:07,760 --> 00:06:10,280
+انا لما احكي مثلا ال hematocrit تلاتين، ال
+
+85
+00:06:10,280 --> 00:06:15,910
+hematocrit اربعين، ستة و تلاتين، ستة و تلاتين اش36
+
+86
+00:06:15,910 --> 00:06:21,770
+في المية، اذا هو بنعبر عنه as a percentage، تمام؟
+
+87
+00:06:21,770 --> 00:06:25,990
+اذا بنعبر عنه as a percentage، نسبة مئوية، كام
+
+88
+00:06:25,990 --> 00:06:29,510
+تحتل نسبة ال .. ال RBCs بالنسبة لل holy blood؟ انا
+
+89
+00:06:29,510 --> 00:06:33,610
+بعتبر ان العينة كلها اللي انا جمعتها مية بالمية،
+
+90
+00:06:33,610 --> 00:06:38,650
+كام ال RBCs منها؟فرح نشوف كيف أنا ممكن أقيسها،
+
+91
+00:06:38,650 --> 00:06:43,070
+تمام؟ إذا هذه هي فكرة الفحص، إذا ال principle مبدأ
+
+92
+00:06:43,070 --> 00:06:48,790
+عمل الفحص أو فكرة الفحص أو يعني الافتراض أو نظرية
+
+93
+00:06:48,790 --> 00:06:53,350
+الفحص، مبدأ عمل الفحص هو إن أنا ال hematocrite is
+
+94
+00:06:53,350 --> 00:06:56,630
+usually determined by spinning a blood filled
+
+95
+00:06:56,630 --> 00:07:02,180
+capillary tube in a centrifugeبعمل determination،
+
+96
+00:07:02,180 --> 00:07:07,020
+قياس، معرفة للـ hematocrit من خلال ان انا اعمل
+
+97
+00:07:07,020 --> 00:07:11,700
+عملية spinning، centrifugation، separation،
+
+98
+00:07:11,700 --> 00:07:15,020
+sedimentation، لاحظين المصطلحات دي كلها نفس المعنى
+
+99
+00:07:15,020 --> 00:07:18,180
+لل blood في ال capillary blood في ال capillary،
+
+100
+00:07:18,180 --> 00:07:21,480
+capillary ممتلقة بال blood من خلال ال centrifuge
+
+101
+00:07:21,480 --> 00:07:26,180
+هاي capillary tube أنا عبقتها لتلت أربعها بال
+
+102
+00:07:26,180 --> 00:07:31,410
+bloodسكرتها بيه لكليه أصبحت مغلقة أحد الأطراف مغلق
+
+103
+00:07:31,410 --> 00:07:36,330
+و هذا before centrifuge after centrifuge راح يصير
+
+104
+00:07:36,330 --> 00:07:40,310
+فيه packaging لال RBC's ال RBC's كلها راح قاش
+
+105
+00:07:40,310 --> 00:07:44,890
+تتركز في منطقة واحدة اللي هي منطقة البطم يليها ال
+
+106
+00:07:44,890 --> 00:07:47,830
+buffy coat و يليها ال plasma هاي ال plasma هذه
+
+107
+00:07:47,830 --> 00:07:53,260
+كلها plasma تمام؟طبعا فأنا بدي أشوف كام تحتل ال
+
+108
+00:07:53,260 --> 00:07:57,600
+RBC بالنسبة ل ال whole blood لو افترضت ان هذه كلها
+
+109
+00:07:57,600 --> 00:08:02,600
+100% كام ال RBC من ال 100% هدول طبعا طيب العينة
+
+110
+00:08:02,600 --> 00:08:07,280
+تبعتي باخد اما .. اما venous blood يعني اما دم من
+
+111
+00:08:07,280 --> 00:08:12,350
+الوريد بصحبه و .. او من ال skin punctureطبعا إذا
+
+112
+00:08:12,350 --> 00:08:16,790
+أنا سحبت دم من الوريد وحطيته في edita tube في ال
+
+113
+00:08:16,790 --> 00:08:21,070
+tube اللي اللونها lavender ال cap تبعها لونه mauve
+
+114
+00:08:21,070 --> 00:08:24,950
+lavender طبعا هذا بيكون فيها edita anticoagulant
+
+115
+00:08:24,950 --> 00:08:31,720
+طبعا فإذا أنا حطيته فيهابس ممكن بعدها أعبي أو أملأ
+
+116
+00:08:31,720 --> 00:08:36,060
+capillary من خلاله أو ممكن مباشرة زي هنا في الصورة
+
+117
+00:08:36,060 --> 00:08:40,100
+من ال skin puncture أملأ ال capillary tube بس في
+
+118
+00:08:40,100 --> 00:08:44,040
+عندي شغلة ال capillary tube لو أنا هنا استعرضت
+
+119
+00:08:44,040 --> 00:08:48,900
+معاكم كيف شكل ال capillary tube و كيف أنواعها هاي
+
+120
+00:08:48,900 --> 00:08:52,640
+زي ما انتوا شايفين هنا في عندي capillary tube
+
+121
+00:08:55,110 --> 00:08:58,450
+هيها، هادى ال capillary tube هيك بتكون، طبعا بتكون
+
+122
+00:08:58,450 --> 00:09:03,510
+عندي علبة يعني فيها أكتر، يعني تقريبا ميت tube ال
+
+123
+00:09:03,510 --> 00:09:07,370
+capillary tube هاي بنات ده طبعا إلها color code
+
+124
+00:09:07,370 --> 00:09:12,670
+نفس فكرة الأنابيب وكل لون يعني إن هي في الداخل
+
+125
+00:09:12,670 --> 00:09:17,340
+ممسوحة بنوع معين من ال anticoagulantتمام؟ هاي اللي
+
+126
+00:09:17,340 --> 00:09:21,580
+لونها أحمر ممسوحة من الداخل بيه برين فهذه تستخدم
+
+127
+00:09:21,580 --> 00:09:26,620
+مباشرة ل ال blood اللي .. اللي من .. مجموع من skin
+
+128
+00:09:26,620 --> 00:09:31,000
+puncture لكن مثلا في هاي عندي نوع آخر من ال
+
+129
+00:09:31,000 --> 00:09:38,090
+capillary زي اللي لونهاblue طبعا، blue فهدي النوع
+
+130
+00:09:38,090 --> 00:09:41,730
+من الكابلري فاضي مافيش فيه إشي مش ممسوح بالداخل ب
+
+131
+00:09:41,730 --> 00:09:44,790
+anticoagulant بسميها bland احنا اتفقنا إذا مافيش
+
+132
+00:09:44,790 --> 00:09:48,270
+فيه إشي retube بسميه bland نفس الإشي الكابلري
+
+133
+00:09:48,270 --> 00:09:52,470
+لإنها مش .. من الداخل مافيش فيها anticoagulant فهي
+
+134
+00:09:52,470 --> 00:09:57,780
+blandcabulary طبعا هذه بجمع فيها أو بملقها من الدم
+
+135
+00:09:57,780 --> 00:10:01,780
+اللي من إيدي تتيوب يعني لو أنا جمعت blob من الوريد
+
+136
+00:10:01,780 --> 00:10:05,300
+و حطيته في إيدي تتيوب بعدين بدي أملق capillary و
+
+137
+00:10:05,300 --> 00:10:09,840
+أشتغل micro hematocrit بستخدم اللي بلان لكن لو بدي
+
+138
+00:10:09,840 --> 00:10:13,680
+أعمل skin puncture و مباشرة أملق capillary من ال
+
+139
+00:10:13,680 --> 00:10:16,660
+skin puncture بستخدم ال heparinase دي اللي لونها
+
+140
+00:10:16,660 --> 00:10:23,540
+أحمر طبعا مابنفعما بنفع أنا أجمع عينة blood في
+
+141
+00:10:23,540 --> 00:10:28,080
+إيديتا تيوب و أروح أستخدم الهيبرينized capillary
+
+142
+00:10:28,080 --> 00:10:33,800
+معاها يعني ما بنفع .. ما بنفع أنا .. ما بنفع أمرر
+
+143
+00:10:33,800 --> 00:10:37,580
+ال blood على نوعين anticoagulant بالأول إيديتا في
+
+144
+00:10:37,580 --> 00:10:42,460
+تيوب بعدين الهيبرين في ال capillaryممنوع ليش؟ لإن
+
+145
+00:10:42,460 --> 00:10:48,060
+هادي ال anticoagulant عبارة عن salts أملاح طبعا
+
+146
+00:10:48,060 --> 00:10:54,000
+طالما إن هي أملاح وجدت في العينة راح تأثر على
+
+147
+00:10:54,000 --> 00:10:57,980
+الضغط الأسموزي على ال osmolality للعينة والضغط
+
+148
+00:10:57,980 --> 00:11:03,080
+الأسموزي للخلايا فبما إنها أثرت على الضغط الأسموزي
+
+149
+00:11:03,080 --> 00:11:07,640
+للخلايا يعني اختلف شكل الخلايا طبعا اختلف شكل
+
+150
+00:11:07,640 --> 00:11:13,910
+الخلاياو طالما اختلف شكلها إذا راح تتأثر قراءة ال
+
+151
+00:11:13,910 --> 00:11:18,700
+hematocratو راح نشرح حد لاحقا خلال المحاضرات
+
+152
+00:11:18,700 --> 00:11:23,320
+الجاية طبعا ال .. ال .. ال specimen لازم انا
+
+153
+00:11:23,320 --> 00:11:26,620
+العينة تبعتي سواء كان يعني specimen هي نفسها
+
+154
+00:11:26,620 --> 00:11:30,900
+معناها sample نفس الاشي عينة طبعا العينة تبعتي
+
+155
+00:11:30,900 --> 00:11:34,200
+لازم انا افصلها باستخدام ال centrifuge should be
+
+156
+00:11:34,200 --> 00:11:37,820
+centrifuged within six hours of collection يعني
+
+157
+00:11:37,820 --> 00:11:43,940
+خلال ست ساعاتمن جمع العينة لازم انا اكون اعمل
+
+158
+00:11:43,940 --> 00:11:50,140
+centrifugation لازم اكون عملت centrifugation ليش؟
+
+159
+00:11:50,140 --> 00:11:55,260
+لأنه اذا انا بدي اتأخر اكتر من هيك ممكن تتأثر يعني
+
+160
+00:11:55,260 --> 00:12:00,540
+تتغيرمكونات العينة، في خلايا ممكن تتكسر، تتحلل،
+
+161
+00:12:00,540 --> 00:12:03,560
+يصير في عندي فاقد في الخلايا، وأنا أصلا قراءتي هي
+
+162
+00:12:03,560 --> 00:12:06,560
+عبارة عن نسبة خلايا، حجم اللي بتحتل الخلايا
+
+163
+00:12:06,560 --> 00:12:10,160
+بالنسبة لل holy blood، فإذا صار في عندي فاقد، إذن
+
+164
+00:12:10,160 --> 00:12:14,680
+هيكون في عندي يعني فاقد أيضا في القراءة، في عندي
+
+165
+00:12:14,680 --> 00:12:21,060
+نسبة قراءة ناقصة، يعني decreased، طبعا، في قراءة
+
+166
+00:12:21,060 --> 00:12:25,920
+ال hematocritHemolyse sample can't be used for
+
+167
+00:12:25,920 --> 00:12:31,300
+testing عينة تمت .. يعني صار فيها تكسر في الخلايا
+
+168
+00:12:31,300 --> 00:12:36,260
+أيضا مش proper مش جيدة لحتى أنا أشتغل فيها
+
+169
+00:12:36,260 --> 00:12:41,500
+hematocrit why؟ لإن ال hemolyse sample بيصير فيه
+
+170
+00:12:41,500 --> 00:12:45,240
+فقد عندي في الخلايا اتكسرت خلايا ال RBCs طب أنا
+
+171
+00:12:45,240 --> 00:12:50,140
+أصلا عم بحسب حجم ال RBCs حجم ال erythrocyte
+
+172
+00:12:50,140 --> 00:12:55,140
+بالنسبة لل holy bloodطالما صار في عندي فقد في هذه
+
+173
+00:12:55,140 --> 00:12:59,480
+الخلايا اتكسرت معناها ايضا القراءة تبعتي راح يصير
+
+174
+00:12:59,480 --> 00:13:03,560
+فيها ايش؟ راح تكون .. تصير minus، في نقص فيها،
+
+175
+00:13:03,560 --> 00:13:07,440
+يعني في decrease في القراءة وهي مشكلة، ال decrease
+
+176
+00:13:07,440 --> 00:13:11,360
+مش .. مش جاية من حالة المريض، لأ هي جاية من ال
+
+177
+00:13:11,360 --> 00:13:15,040
+technical error تبعي، انه انا اتأخرت على العينة،
+
+178
+00:13:15,040 --> 00:13:17,700
+اتعاملت مع العينة بشكل خاطئ فصار في تكسر في
+
+179
+00:13:17,700 --> 00:13:25,010
+الخلايا وها كدهطيب إيش بلزمني أدوات ومواد لحتى
+
+180
+00:13:25,010 --> 00:13:28,610
+أشتغل ال hematocrit؟ أول إشي بلزمني capillary tube
+
+181
+00:13:28,610 --> 00:13:33,050
+إما تكون heparinized يعني red tip احنا حكينا إن
+
+182
+00:13:33,050 --> 00:13:39,210
+اللي معلمة بي ال red بالخط الأحمر من طرفها هدي
+
+183
+00:13:39,210 --> 00:13:44,530
+فيها heparin أما إذا كانت bland فهي blue يعني
+
+184
+00:13:44,530 --> 00:13:47,830
+بتكون blue tip يعني إذا كانت فاضية فهي blue أربطها
+
+185
+00:13:47,830 --> 00:13:56,780
+بي blandP Plain و P Blue Plain فلونها blue احفظوها
+
+186
+00:13:56,780 --> 00:14:01,500
+هك تمام؟ ومحتاجة clay اللي حكينا عنه المعجونة او
+
+187
+00:14:01,500 --> 00:14:05,420
+الطينة و ال micro hematocrit centrifuge محتاجة
+
+188
+00:14:05,420 --> 00:14:09,620
+readerتدريج خاص لحتى أقرأ عليه لحتى أعتبر أن
+
+189
+00:14:09,620 --> 00:14:13,940
+العينة كلها 100 و أشوف نسبة ال RBC منها هذا بيتم
+
+190
+00:14:13,940 --> 00:14:20,120
+من خلال تدريج خاص محتاجة جوز شاش طبعا او يعني قطعة
+
+191
+00:14:20,120 --> 00:14:26,500
+كماشية قطنية لحتى انا يعني انظف ال tube اذا تم
+
+192
+00:14:26,500 --> 00:14:30,160
+غمرها ب tube blood اذا انا انظف ال capillary من
+
+193
+00:14:30,160 --> 00:14:34,100
+برا اذا تم غمرها ب tube blood و اتبهدلت من برا انا
+
+194
+00:14:34,100 --> 00:14:38,380
+بدي انظفهاطبعا باستخدام ايش يا بنات؟ Goose شاش
+
+195
+00:14:38,380 --> 00:14:44,260
+يعني اللي هو زي شاش لجروح، تمام؟ طيب هاي زي ما
+
+196
+00:14:44,260 --> 00:14:48,060
+انتوا شايفين في الصورة ال capillary أنواع و كل نوع
+
+197
+00:14:48,060 --> 00:14:51,740
+له color code زي فكرة الأنابيب يا بنات،يلها color
+
+198
+00:14:51,740 --> 00:14:55,920
+code كل نوع معلم بلون زي لون الغطاية بيكون ايضا
+
+199
+00:14:55,920 --> 00:14:59,830
+معلمة لل capillary نفسها في هذا اللونوكل لون يعني
+
+200
+00:14:59,830 --> 00:15:03,930
+محتوها مختلف احنا بس هلاقى حاليا المطلوب مننا انه
+
+201
+00:15:03,930 --> 00:15:09,070
+نعرف ان ال red فيها heparin و لبلان blue طبعا هذا
+
+202
+00:15:09,070 --> 00:15:12,930
+اللي بدياكم تعرفو حاليا و بدياكم تعرفوا اذا العينة
+
+203
+00:15:12,930 --> 00:15:16,350
+جاية من skin puncture معناه انا بدي استخدم ال
+
+204
+00:15:16,350 --> 00:15:21,350
+heparinized capillary tubeوإذا العينة جاية من vain
+
+205
+00:15:21,350 --> 00:15:25,390
+من وريد و أنا فضيتها في إيدي تتيوب و بدى أملأ
+
+206
+00:15:25,390 --> 00:15:28,970
+capillary بستخدم bland لإن العينة already صارت
+
+207
+00:15:28,970 --> 00:15:32,410
+anticoagulated already مضعف عليها anticoagulant
+
+208
+00:15:32,410 --> 00:15:35,810
+اللي هو الإيدي تعالى فمش محتاجة أنا أصلا أستخدم
+
+209
+00:15:35,810 --> 00:15:41,120
+capillary أيضا فيها anticoagulant تمام؟طيب، إيش هي
+
+210
+00:15:41,120 --> 00:15:45,840
+ال procedure التجري ده اللي أنا بستخدمها أو ب ..
+
+211
+00:15:45,840 --> 00:15:50,620
+بجري من خلالها ال hematocrit؟ أول إشي بيحكيلك في
+
+212
+00:15:50,620 --> 00:15:54,380
+ال two capillary tube approximately three quarters
+
+213
+00:15:54,380 --> 00:15:59,130
+full with blood anticoagulatedeither with edita or
+
+214
+00:15:59,130 --> 00:16:03,710
+with hiberin يعني بدي أملأ two capillary tube
+
+215
+00:16:03,710 --> 00:16:08,410
+تقريبا لتلت أرباعهم بال blood اللي anticoagulated
+
+216
+00:16:08,410 --> 00:16:12,190
+اللي مضعف عليه anticoagulant إما edita أو إذا كان
+
+217
+00:16:12,190 --> 00:16:15,290
+أنا بستخدم حكاية skin puncture على طول باخد ال
+
+218
+00:16:15,290 --> 00:16:19,930
+hiberin طيب ال hiberinized capillary tubeهلأ ال
+
+219
+00:16:19,930 --> 00:16:23,910
+blood for hiperionized capillary tube حكينا أنه
+
+220
+00:16:23,910 --> 00:16:26,950
+إيش من إيش أنا باخده من ال puncture من ال skin
+
+221
+00:16:26,950 --> 00:16:31,650
+puncture لكن اللي من ال edita بفضيه في plain
+
+222
+00:16:31,650 --> 00:16:36,390
+capillary tube هذه الجزئية أنا كررتها كذا مرة لحتى
+
+223
+00:16:36,390 --> 00:16:42,010
+أنه تفهموها لأنها مهمة يا بناتطيب بعد ما أنا أملأ
+
+224
+00:16:42,010 --> 00:16:45,370
+ال tube بتلات .. بتلات أربعها طبعا حكينا إن ال
+
+225
+00:16:45,370 --> 00:16:49,290
+blood بيملأها بالخاصية الشعرية capillary action
+
+226
+00:16:49,290 --> 00:16:53,450
+seal the end of the tube with the non-colouring
+
+227
+00:16:53,450 --> 00:17:00,510
+ring with the non-absorbent clay هلأ يعني إنه من
+
+228
+00:17:00,510 --> 00:17:07,750
+الجهة المش ملونة مثلا سكري ال capillary بالclay
+
+229
+00:17:07,750 --> 00:17:16,040
+يعنيفوتي .. يعني هيك ..فوتيها في داخل ال container
+
+230
+00:17:16,040 --> 00:17:20,560
+او يعني bottle او cup موجود فيه ال clay فهتلاحظى
+
+231
+00:17:20,560 --> 00:17:24,820
+انه ال clay هيدخل جوا ال .. ال capillary و يسكر
+
+232
+00:17:24,820 --> 00:17:29,940
+الفتحة احد الطرفين balance the tube in the
+
+233
+00:17:29,940 --> 00:17:32,600
+centrifuge with the clay طبعا انا بستخدم two
+
+234
+00:17:32,600 --> 00:17:36,520
+capillary للعينة الواحدة why؟ او ليش؟ عشان انا
+
+235
+00:17:36,520 --> 00:17:40,550
+اعمل ال .. ال .. ال tube بدها balance تمام؟تاني
+
+236
+00:17:40,550 --> 00:17:44,530
+إشي لإنه ب .. بجيب القراءة على ال two capillaries
+
+237
+00:17:44,530 --> 00:17:49,670
+tube و بعدين ب .. بجيب ال average لإلهم و طالما
+
+238
+00:17:49,670 --> 00:17:52,170
+أنا جبت ال average لقراقتين معناها أنا more
+
+239
+00:17:52,170 --> 00:17:57,280
+accurate and preciseبحط الأنابيب في ال centrifuge
+
+240
+00:17:57,280 --> 00:18:03,100
+مقابل بعضها البعض و بعد هيك بشغل جهاز ال
+
+241
+00:18:03,100 --> 00:18:07,040
+centrifuge لمدة خمس دقائق على سرعة ما بين العشر
+
+242
+00:18:07,040 --> 00:18:12,060
+ألف إلى خمستاشر ألف round per minuteuse the brake
+
+243
+00:18:12,060 --> 00:18:14,980
+to stop the centrifuge ممنوع أنا أستخدم ال brake
+
+244
+00:18:14,980 --> 00:18:19,060
+لحتى أوقف ال centrifuge لإنه إذا حكينا إحنا قبل
+
+245
+00:18:19,060 --> 00:18:23,080
+هيك إنه انا إذا عملت إيقاط مفاجئ لل centrifuge
+
+246
+00:18:23,080 --> 00:18:27,920
+معناها ال sediment اللي اترسب راح يندفع باتجاه
+
+247
+00:18:27,920 --> 00:18:30,780
+الأعلى فبالتالي راح يصير في mixing للعينة أو
+
+248
+00:18:30,780 --> 00:18:34,520
+انفصال لل sediment وهذا راح يعطيني error خطأ في
+
+249
+00:18:34,520 --> 00:18:40,050
+القراءةطبعا بعد ما يتوقف الجهاز لأ يعني بعد ما
+
+250
+00:18:40,050 --> 00:18:43,670
+ينتهي يقول خمسة minute بيرن جرس يعني عشان تنبيه
+
+251
+00:18:43,670 --> 00:18:49,870
+أنه انتهت المدة وبيبدأ الجهاز يبطئ سرعته مع نفسه
+
+252
+00:18:49,870 --> 00:18:56,550
+طبعا إلى أن يقف يعني to stopبعد ما الجهاز يعني ت
+
+253
+00:18:56,550 --> 00:19:03,070
+stop يقف ب .. انا بفتح ال .. ال cover تبعه و باخد
+
+254
+00:19:03,070 --> 00:19:07,770
+القنابيب و باستخدام تدريج خاص ب .. بعمل
+
+255
+00:19:07,770 --> 00:19:10,430
+determination لل hematocrit، بعرف ال hematocrit
+
+256
+00:19:13,130 --> 00:19:16,330
+كيف هذا .. فكرة ال .. ال reader تبعي؟ زي ما انتوا
+
+257
+00:19:16,330 --> 00:19:20,330
+شايفين هو عبارة عن تدريج بيبدأ بالصفر و بينتهي
+
+258
+00:19:20,330 --> 00:19:26,090
+بالمية، طبعا؟ هذا التدريج بتشوفوه زي عم بيتسع كل
+
+259
+00:19:26,090 --> 00:19:30,330
+ما اتجهنا بهذا الاتجاه، طبعا؟ ضيق هنا و بيتسع هنا
+
+260
+00:19:30,330 --> 00:19:34,830
+و نفس الفكرة انه ال .. ال .. بيدعته صفر و نهايته
+
+261
+00:19:34,830 --> 00:19:39,080
+ميةطب ليش .. ليش عم بالتساع؟ لحتى يناسب جميع
+
+262
+00:19:39,080 --> 00:19:42,280
+الأطوال و جميع محتويات العينة يعني انا لو .. لو
+
+263
+00:19:42,280 --> 00:19:46,300
+عينة طويلة أو تيوب طويلة أو عينة قصيرة، تيوب
+
+264
+00:19:46,300 --> 00:19:49,440
+مالقتها كتير، تيوب ما .. ما .. ما عبيتها كتير
+
+265
+00:19:49,440 --> 00:19:53,640
+فبالتالي انه في النهاية يكون التدريج مناسب لإلها،
+
+266
+00:19:53,640 --> 00:19:56,040
+لا تيوب طويلة و الله بروح على الجهة الطويلة من
+
+267
+00:19:56,040 --> 00:20:01,540
+التدريج، تيوب قصيرة أو يعني تيوب محتواها قليل،
+
+268
+00:20:01,540 --> 00:20:06,260
+بروح على الجهة القصيرة من التدريجبروح بطابق يعني
+
+269
+00:20:06,260 --> 00:20:09,140
+زي ما انتوا شايفين بداية ال blood لازم تكون عند خط
+
+270
+00:20:09,140 --> 00:20:13,720
+الصفر و نهاية ال plasma لازم تكون متابقة لخط
+
+271
+00:20:13,720 --> 00:20:18,340
+المياهبعدين بشوف ال RBCs و بقرأ من تحت ال buffer
+
+272
+00:20:18,340 --> 00:20:23,880
+code بشوف هي مقابل لأي قراءة و بعرف كام القراءة ال
+
+273
+00:20:23,880 --> 00:20:27,820
+RBCs يعني كام نسبة ال RBCs بالنسبة لل holy blood
+
+274
+00:20:27,820 --> 00:20:32,920
+تمام؟ هي فكرة استخدام التدريج عندنا أنواع و ..
+
+275
+00:20:32,920 --> 00:20:35,860
+يعني أشكال مختلفة من التدريج راح ان شاء الله بس
+
+276
+00:20:35,860 --> 00:20:38,700
+نرجع المختبرات راح أعلمكم عليها و كيف تستخدموها
+
+277
+00:20:38,700 --> 00:20:44,640
+تمام؟بحكيلي وانت بتقرا ايه؟ don't include the
+
+278
+00:20:44,640 --> 00:20:48,020
+buffy coat, leukocyte and the platelet why؟ احنا
+
+279
+00:20:48,020 --> 00:20:51,260
+بنقرا micro hematocrit ال volume occupied by
+
+280
+00:20:51,260 --> 00:20:57,520
+erythrocyte by erythrocyte طبعا؟ الحجم اللي بتحتله
+
+281
+00:20:57,520 --> 00:21:03,330
+ال RBCs فقط لغانفلذلك انا ما بدخل .. ما .. ال ..
+
+282
+00:21:03,330 --> 00:21:06,610
+ال .. يعني مابحسب ال .. ال buffy code في القراءة
+
+283
+00:21:06,610 --> 00:21:10,210
+don't include the buffy code in the reading تمام؟
+
+284
+00:21:10,210 --> 00:21:13,970
+ممنوع تدرجي او يعني تحسبي ال buffy code داخل
+
+285
+00:21:13,970 --> 00:21:18,170
+القراءة طبعتك انا بقرأ من تحت ال buffy code تمام؟
+
+286
+00:21:18,170 --> 00:21:21,490
+اللازم ال tubes .. ال tubes تبعتي ال two tubes
+
+287
+00:21:21,490 --> 00:21:26,160
+يكون على الأقليعني او يعني على .. على اكتر الفرق
+
+288
+00:21:26,160 --> 00:21:29,680
+ما بينهم اتنين في المية، ايش يعني؟ يعني ممكن يكون
+
+289
+00:21:29,680 --> 00:21:33,740
+الفرق ما بينهم نصف في المية، واحد في المية، اتنين
+
+290
+00:21:33,740 --> 00:21:37,180
+في المية، plus minus طبعا يا بنات، اكتر من هيك
+
+291
+00:21:37,180 --> 00:21:40,320
+ممنوع، يعني plus minus تلاتة لأ، plus minus اتنين
+
+292
+00:21:40,320 --> 00:21:44,100
+و نصف لأ، معناه في variation كتير في النتائج هيك،
+
+293
+00:21:44,100 --> 00:21:46,140
+انا ممنوع .. مش لازم يكون في عندي variation، لازم
+
+294
+00:21:46,140 --> 00:21:50,830
+يكون في تقارب في النتائجتمام، إيش يعني الكلام هذا؟
+
+295
+00:21:50,830 --> 00:21:54,510
+لنفرض إنه في عندي tube اللي hematocrite فيها طلع
+
+296
+00:21:54,510 --> 00:22:00,910
+36%، تمام؟ معناه ال tube التانية كام لازم يطلع؟
+
+297
+00:22:00,910 --> 00:22:06,330
+ممكن يطلع 36 زيها بالظبط، ممكن 36.5 أو 35.5 يعني
+
+298
+00:22:06,330 --> 00:22:11,250
+plus minus نص، تمام؟ممكن يكون خمسة و تلاتين أو
+
+299
+00:22:11,250 --> 00:22:14,890
+سبعة و تلاتين plus minus واحد هيك تمام؟ ممكن
+
+300
+00:22:14,890 --> 00:22:19,830
+يكونوا اربعة و تلاتين او تمانية و تلاتين هيك plus
+
+301
+00:22:19,830 --> 00:22:25,170
+minus اتنين بس نهائيا .. نهائيا ما بقبل نتيجة اذا
+
+302
+00:22:25,170 --> 00:22:29,130
+كانت واحدة فيهم قيمتها ستة و تلاتين مثلا و القيمة
+
+303
+00:22:29,130 --> 00:22:31,610
+التانية تلاتة و تلاتين يعني في فرق ما بينهم تلاتة
+
+304
+00:22:31,610 --> 00:22:34,910
+في المية او اذا كانت ستة و تلاتين و التانية تسعة و
+
+305
+00:22:34,910 --> 00:22:38,030
+تلاتين في فرق ما بينهم تلاتة في المية غلطلازم
+
+306
+00:22:38,030 --> 00:22:40,910
+within اتنين في المية يكون الفارق ما بينهم علشان
+
+307
+00:22:40,910 --> 00:22:45,790
+احكي و الله انه في تقارب في نتيجة تبعتي تمام؟ باخد
+
+308
+00:22:45,790 --> 00:22:49,530
+القيمتين بقرأ ال .. ال .. ال two tubes و بجيب ال
+
+309
+00:22:49,530 --> 00:22:52,870
+average تبعهم علشان اكون more accurate and precise
+
+310
+00:22:54,880 --> 00:22:59,440
+طيب خلّينا بس نشوف كيف شكل ال centrifuge اللي احنا
+
+311
+00:22:59,440 --> 00:23:02,160
+.. احنا حكينا ان في عندي micro hematocrit
+
+312
+00:23:02,160 --> 00:23:05,840
+centrifuge هاي شكل ال centrifuge طبعا هذا typical
+
+313
+00:23:05,840 --> 00:23:09,380
+موجود عندنا بالمختبرات زيه، typically، يعني أخوه،
+
+314
+00:23:09,380 --> 00:23:13,710
+نفس الألوان، نفس الشكلهذا ال cover الخارجي و في
+
+315
+00:23:13,710 --> 00:23:18,710
+cover داخلي تمام و هذا تدريج timer هو already set
+
+316
+00:23:18,710 --> 00:23:22,330
+على سرعة معينة يعني انا مابحدد سرعته هذا الجرس
+
+317
+00:23:22,330 --> 00:23:26,590
+الإنذار انه يعني توقف الجهاز و هذا ال break تمام
+
+318
+00:23:26,590 --> 00:23:30,730
+لو جيت انا فتحت ال cover الخارجي و الداخلي كيف رح
+
+319
+00:23:30,730 --> 00:23:35,860
+يكون شكل ال .. ال .. ال hematocritزي ما انتوا
+
+320
+00:23:35,860 --> 00:23:41,320
+شايفين بهذا الشكل بيكون شكل ال .. ال .. ال .. ال
+
+321
+00:23:41,320 --> 00:23:45,240
+micro hematocrit centrifuge من الداخل ال .. هذه ال
+
+322
+00:23:45,240 --> 00:23:49,680
+head وهي فيها buckets فيها فرزات تمام؟ هذا الفرز
+
+323
+00:23:49,680 --> 00:23:52,980
+مخصص لل capillary هذا عشان هيك أنا بسميه
+
+324
+00:23:52,980 --> 00:23:56,520
+centrifuge خاص بال hematocrit التيوب لإن هو
+
+325
+00:23:56,520 --> 00:23:59,940
+مابيستوعبش تيوبات كبيرة هو بيستوعب فقط ال .. ال ..
+
+326
+00:23:59,940 --> 00:24:06,200
+ال micro hematocrit تمام؟ طيبهدا بس ال .. ال ..
+
+327
+00:24:06,200 --> 00:24:10,280
+الشكل ال micro centrifuge أو ال micro hematocrite
+
+328
+00:24:10,280 --> 00:24:13,900
+ال centrifuge من الداخل هاي كمان صورة أخرى لإله
+
+329
+00:24:13,900 --> 00:24:18,580
+شايفين؟ هاد .. هاد ال central shaft هادي ال head
+
+330
+00:24:19,040 --> 00:24:24,000
+وفيها buckets طبعا؟ انتوا شوفتوا ال buckets في ال
+
+331
+00:24:24,000 --> 00:24:28,940
+centrifuges التانية عبارة عن مكان زي وعاء بيشبه
+
+332
+00:24:28,940 --> 00:24:35,820
+شكل ال tube طبعا؟ هذا أيضا فرز او زي مكان مخصص
+
+333
+00:24:35,820 --> 00:24:39,780
+بيشبه شكل ال capillary بيشبه شكل ال capillary tube
+
+334
+00:24:41,240 --> 00:24:46,060
+لذلك هو مخصص لـ micro-hematocrit هذا الجهاز للـ
+
+335
+00:24:46,060 --> 00:24:48,600
+centrifuge هذه شايفين هي الغطاية الداخلية وهي
+
+336
+00:24:48,600 --> 00:24:52,140
+الغطاية الخارجية طبعا يا بنات لما بحط لي tubes
+
+337
+00:24:52,140 --> 00:24:57,760
+لازم أراعي ال balance كل tube مقابل التانية كل
+
+338
+00:24:57,760 --> 00:25:02,940
+tube مقابل التانيةتمام؟ طيب و في شغلة لازم
+
+339
+00:25:02,940 --> 00:25:08,160
+أنتبهلها أنه لما أسكر ال tube بال clay لازم
+
+340
+00:25:08,160 --> 00:25:12,840
+المنطقة المغلقة وين تكون؟ تكون على الحواف تمام؟
+
+341
+00:25:12,840 --> 00:25:16,580
+تكون هنا المنطقة المغلقة أو هذه المنطقة المفتوحة
+
+342
+00:25:16,910 --> 00:25:21,910
+ليش بقى انتبه لهاي الشغلة؟ لإنه هذا جهاز طارد
+
+343
+00:25:21,910 --> 00:25:24,830
+مركزي، الأجزاء .. الأجزاء الثقيلة وين راح تروح؟
+
+344
+00:25:24,830 --> 00:25:27,890
+راح تحدد تروح بعيد عن ال central shaft، بعيد عن
+
+345
+00:25:27,890 --> 00:25:32,210
+المركز، وين راح تقى تمطردها؟ بهذا الاتجاه، يعني
+
+346
+00:25:32,210 --> 00:25:36,380
+هنا ال RBCs راح تتجمعاتخيلي لو المكان هذا مفتوح مش
+
+347
+00:25:36,380 --> 00:25:40,080
+مسكر بالـ Clay، إيش يعني؟ يعني راح يصير .. يصير
+
+348
+00:25:40,080 --> 00:25:42,960
+طردها خالص، يعني إحنا طردناها و زيادة و كت ..
+
+349
+00:25:42,960 --> 00:25:49,160
+عملنا و كترنا، فبصير إيش؟ طردنا ال .. ال .. ال ..
+
+350
+00:25:49,160 --> 00:25:54,040
+ال RBCs خارج التيوب و ليس at the bottom of التيوب
+
+351
+00:25:54,540 --> 00:25:58,420
+طبعا فعشان هيك لازم المنطقة المغلقة تكون في هذا
+
+352
+00:25:58,420 --> 00:26:02,920
+الاتجاه عشان لما تيجي ال RBCs يتم طردها مركزيا او
+
+353
+00:26:02,920 --> 00:26:06,120
+يعني تترثب او يصير لها sedimentation separation في
+
+354
+00:26:06,120 --> 00:26:09,620
+at the bottom of the tube يكون فيها عائق على الأقل
+
+355
+00:26:09,620 --> 00:26:15,380
+يمنعها من انها هي يصير لها loss فقدان طبعا اتخيلي
+
+356
+00:26:15,380 --> 00:26:19,150
+لو انا عكستو في .. بيصير في طارد مركزي إيش يعني؟
+
+357
+00:26:19,150 --> 00:26:22,270
+إن أنا مساكت الأتيوب و كباتها، هاي معناها، و كأنك
+
+358
+00:26:22,270 --> 00:26:27,010
+يا أبو زاد ما غزيت، تمام؟ أنا بكون كبات إيدي ..
+
+359
+00:26:27,010 --> 00:26:34,860
+أنا بيبي بإيدياطيب ال .. ال .. ال cover الداخلي
+
+360
+00:26:34,860 --> 00:26:38,740
+هذا بيغلق .. بيتم إغلاقه من خلال ترس يا بنات سم
+
+361
+00:26:38,740 --> 00:26:44,440
+يعني بيحبك على ال capillary tube بعدين بسكر ال
+
+362
+00:26:44,440 --> 00:26:48,160
+cover الخارجي عشان هيك لازم أتأكد أن كل tube
+
+363
+00:26:48,160 --> 00:26:51,280
+موجودة داخل الفرز المخصص لإلها مش طالع على بره أو
+
+364
+00:26:51,280 --> 00:26:55,520
+برزه لإنه إذا كانت مش موجودة بال .. بالفرز يعني
+
+365
+00:26:55,520 --> 00:27:00,310
+تماما موجودة داخل الفرز المخصص لإلهاهاي ال .. ال
+
+366
+00:27:00,310 --> 00:27:05,050
+.. الغطاية لما أجي أسكرها و تحبك راح تطحم ال tube
+
+367
+00:27:05,050 --> 00:27:09,530
+ال cabillary راح تكسرها، تفتفتها، طبعا؟ و هيك أنا
+
+368
+00:27:09,530 --> 00:27:15,630
+بكون فقدت العينة تبعتيفإذا احذروا ان تفقدوا العينة
+
+369
+00:27:15,630 --> 00:27:20,430
+تبعتكم اتأكدي ان ال .. ال tube جالسة بالمكان
+
+370
+00:27:20,430 --> 00:27:25,350
+المخصص لإلها بالفرز المخصص لإلها ومنطقة المغلقة
+
+371
+00:27:25,350 --> 00:27:29,990
+بال clay هي اللي موجودة outside بعيدة عن المركز و
+
+372
+00:27:29,990 --> 00:27:34,590
+المنطقة المفتوحة هي اللي أقرب ما يكون لمركزها تمام
+
+373
+00:27:36,940 --> 00:27:41,140
+طيب بعد ما أنا خلصت وحاكينا ب .. ب .. بحطها على
+
+374
+00:27:41,140 --> 00:27:46,400
+التدريج الخاص فيها و بحط بداية ال blood عند خط ال
+
+375
+00:27:46,400 --> 00:27:50,960
+zero و نهاية ال plasma عند خط المية بقرا ال .. ال
+
+376
+00:27:50,960 --> 00:27:56,860
+.. نسبة ال RBCs بالنسبة يعني لل whole blood و من
+
+377
+00:27:56,860 --> 00:27:59,680
+تحت ال buffy quote بقرا يعني أنا بس بدي نسبة ال ..
+
+378
+00:27:59,680 --> 00:28:04,430
+ال erythrocyte فقط لا غيرطيب يا بنات هذه ال
+
+379
+00:28:04,430 --> 00:28:08,550
+reference ranges يعني ال normal range حكينا إنه ال
+
+380
+00:28:08,550 --> 00:28:14,070
+normal value أنا بعبّر عنها ب range و ليس قيمة
+
+381
+00:28:14,070 --> 00:28:18,890
+واحدة ل newborn إيش قيمة الهيمتكرة الطبيعية عندهم
+
+382
+00:28:18,890 --> 00:28:26,530
+من 53% ل 65%؟ طب ليش أنا بحكي 53% ل 65%؟ هي عالية
+
+383
+00:28:26,530 --> 00:28:29,970
+أنتم لاحظيني أنا مقارنة بالقيم التانية هي أعلى إشي
+
+384
+00:28:30,550 --> 00:28:34,990
+حكينا لإنه ال newborn الأطفال حديثي الولادة
+
+385
+00:28:34,990 --> 00:28:39,030
+بيولدوا عندهم نسبة ال hemoglobin عالية كتير و ال
+
+386
+00:28:39,030 --> 00:28:42,310
+RBCs قيمتها بتكون عالية كتير يعني عندهم خلايا
+
+387
+00:28:42,310 --> 00:28:46,750
+عالية عندهم خلايا كتيرة ليش؟ لإنه أول حاجة هم في
+
+388
+00:28:46,750 --> 00:28:50,810
+حالة growth burst يعني انفجار في النمو عندهم يعني
+
+389
+00:28:50,810 --> 00:28:56,030
+هم في حالة نمو هائل تمام؟ و فمحتاجين oxygen
+
+390
+00:28:56,030 --> 00:29:03,850
+delivery يعني محتاجينRBCs كتيرة و أيضا عندهم مشكلة
+
+391
+00:29:03,850 --> 00:29:07,470
+ان ال homoglobin تبعهم ال affinity تبعته الجاذبية
+
+392
+00:29:07,470 --> 00:29:10,910
+تبعته للأكسوجين عالية يعني لما بيروح يوصل ال
+
+393
+00:29:10,910 --> 00:29:15,590
+oxygen لل tissue تمام بيكون مش هاين عليه يترك ال
+
+394
+00:29:15,590 --> 00:29:21,140
+oxygen تمام بصعوبة بيترك ال oxygenفبالتالي ربنا
+
+395
+00:29:21,140 --> 00:29:25,640
+عوضهم بزيادة RBCs وزيادة Hemoglobin طبعا إذا في
+
+396
+00:29:25,640 --> 00:29:28,680
+سبب بين أول حاجة أن ال Hemoglobin تبعهم إله
+
+397
+00:29:28,680 --> 00:29:32,420
+affinity عالية للأكسوجين تاني إشي لإنهم في حالة
+
+398
+00:29:32,420 --> 00:29:36,000
+growth burst طبعا خليني أورجيكم إيه كيف .. إيش
+
+399
+00:29:36,000 --> 00:29:42,180
+يعني كيف تنكتب كلمة growth burst طبعا growth burst
+
+400
+00:29:43,530 --> 00:29:47,410
+هدى الحالة اللى توصف وضع النمو عند ال .. ال
+
+401
+00:29:47,410 --> 00:29:53,030
+newborn تمام؟ طيب ال infant او ال child يعني لبس
+
+402
+00:29:53,030 --> 00:29:56,890
+يكبر شوية بتبدأ ايش ال homoglobin تبعهم اتقل ال
+
+403
+00:29:56,890 --> 00:30:01,710
+affinity تبعته لل Oxygen فبتبدأ ايش انه نسبة ال
+
+404
+00:30:01,710 --> 00:30:07,590
+RBCs تبعتهم اتقل ف ال hematocrit بيحتل من 30 ل 43%
+
+405
+00:30:07,590 --> 00:30:12,280
+من حجم ال holy bloodطبعا يا بنات هذا في العينة و
+
+406
+00:30:12,280 --> 00:30:15,220
+يمثل ال blood مش أنا أخدت عينة ممثلة عن ال blood
+
+407
+00:30:15,220 --> 00:30:19,600
+أخدت whole blood من الوريد و الله أخدت 2 cm و لا
+
+408
+00:30:19,600 --> 00:30:24,260
+أخدت 3 cm و لا 4 cm هم بيقش في محتوهم هيعبرلي عن
+
+409
+00:30:24,260 --> 00:30:32,170
+محتوى ال blood كله داخل الجسم طبعناف 30% ل 43%
+
+410
+00:30:32,170 --> 00:30:39,110
+adult male من 42% ل 52% ال adult female من 37% ل
+
+411
+00:30:39,110 --> 00:30:44,350
+47% why ال male أعلى؟ ليش ال adult male أعلى في ال
+
+412
+00:30:44,350 --> 00:30:49,270
+hemotocrit من ال adult female؟ أول إشي لإنه نسبة
+
+413
+00:30:49,270 --> 00:30:53,290
+ال muscle mass الكتلة العضلية عند ال .. ال .. ال
+
+414
+00:30:53,290 --> 00:30:57,740
+male أكترطبعا فهم محتاجين طالما عندهم muscle mass
+
+415
+00:30:57,740 --> 00:31:00,540
+أكتر يعني عندهم خلايا أكتر يعني محتاجين oxygen
+
+416
+00:31:00,540 --> 00:31:06,390
+delivery أكتر فبالتالي ال RBCs عندهم أكترطيب ال
+
+417
+00:31:06,390 --> 00:31:11,790
+adult female نظرا لإنه أيضا نسبة ال RBC قليلة
+
+418
+00:31:11,790 --> 00:31:19,510
+عندهم هم في عندهم regular blood loss every month
+
+419
+00:31:19,510 --> 00:31:24,430
+كل شهر بيصير فيه فقدان لل blood عند ال female من
+
+420
+00:31:24,430 --> 00:31:29,340
+خلال ال menstruation تمام؟ف .. فبالتالي هذا اللي
+
+421
+00:31:29,340 --> 00:31:33,180
+عامل نظرا لإنه عندهم الكتلة العضلية أقل و عندهم
+
+422
+00:31:33,180 --> 00:31:37,760
+فقدان ل ال blood كل .. بشكل شهري فبالتالي ال .. ال
+
+423
+00:31:37,760 --> 00:31:43,280
+.. نسبة ال hematocrita عندهم قليلة، تمام؟ أقل من
+
+424
+00:31:43,280 --> 00:31:47,000
+ال adult male، بس هذا ال normal تبعهم يعني، طيب
+
+425
+00:31:48,750 --> 00:31:55,370
+خلّينا نشوف ال .. إذا هك إحنا بنكون شرحنا كل إشي
+
+426
+00:31:55,370 --> 00:31:59,870
+بتعلق بالتجربة مع ال normal ranges هلأ بنحكي عن ال
+
+427
+00:31:59,870 --> 00:32:04,750
+source of error يعني بعض الملاحظات و بعض ال
+
+428
+00:32:04,750 --> 00:32:08,650
+trickات اللي ممكن تعملي خلل أو تعملي error في
+
+429
+00:32:08,650 --> 00:32:12,770
+النتيجة أو تعطيني قراءة خاطئة لإنسبة ال hematocrit
+
+430
+00:32:13,400 --> 00:32:16,540
+خلينا بس نشوف بالأول هنا الصورة نناقش مع بعض
+
+431
+00:32:16,540 --> 00:32:20,940
+الصورة إذا كانت هذه هي ال normal range ل ال .. ال
+
+432
+00:32:20,940 --> 00:32:25,080
+hematocrit يعني نسبة ال RBCs فإنه أقل من ال .. من
+
+433
+00:32:25,080 --> 00:32:28,460
+ال normal لو كان شخص عنده ال .. يعني هاي ال .. هاي
+
+434
+00:32:28,460 --> 00:32:32,160
+ال reference ranges أنا بقارن بهدول، تمام؟ فإذا
+
+435
+00:32:32,160 --> 00:32:35,800
+كان شخص عنده نسبة ال hematocrit أقل من ال normal،
+
+436
+00:32:35,800 --> 00:32:42,640
+تمام؟ فمعناه هذا الشخص عنده anemiaفقر دم عنده،
+
+437
+00:32:42,640 --> 00:32:45,720
+عنده نسبة ال RBC's قليلة بالنسبة لل holy blood،
+
+438
+00:32:45,720 --> 00:32:50,000
+يعني الخلايا عنده قليلة، فبالتالي خلايا الدم ال
+
+439
+00:32:50,000 --> 00:32:53,940
+RBC's، ال oxygen delivery قليل، عنده hypoxia، عنده
+
+440
+00:32:53,940 --> 00:32:59,790
+فقر دم، أنيمياطب لو كانت نسبة ال RBCs أعلى من
+
+441
+00:32:59,790 --> 00:33:02,690
+الطبيعي، أعلى من ال normal، هاي نفترض أنه هذا ال
+
+442
+00:33:02,690 --> 00:33:07,350
+normal، أعلى منه، بسمي هذه الحالة polycythemia،
+
+443
+00:33:07,350 --> 00:33:12,000
+إيش يعني polycythemia؟ poly يعني عديدكلمة poly
+
+444
+00:33:12,000 --> 00:33:16,360
+عديد خدتوها أكيد في الحيوية مرت عليكم لما ذكر
+
+445
+00:33:16,360 --> 00:33:21,940
+عندكم ال polysaccharide و زي كلمة poly عديد، طبعا؟
+
+446
+00:33:21,940 --> 00:33:25,480
+طيب، polysaccharide، polypeptide، poly .. كذا، أي
+
+447
+00:33:25,480 --> 00:33:31,340
+poly فهو عديد معناها كلمة .. كلمة poly site جاية
+
+448
+00:33:31,340 --> 00:33:36,510
+من كلمة خلايةتمام? site جاي من كلمة خلاية .. site
+
+449
+00:33:36,510 --> 00:33:41,310
+.. cytology علم الخلاية .. site خلاية .. emia كأي
+
+450
+00:33:41,310 --> 00:33:46,790
+كلمة بتنتهي ب emia يعني في الدم، تمام؟ يعني زي عدي
+
+451
+00:33:46,790 --> 00:33:50,450
+.. خلاية عديدة في الدم، يعني زيادة عدد خلاية الدم
+
+452
+00:33:50,450 --> 00:33:55,660
+ال RBCsتمام؟ هاي معناه كلمة polycythemia طيب يا
+
+453
+00:33:55,660 --> 00:33:59,780
+بنات إيش يعني polycythemia؟ في بعض الحالات طبعا هي
+
+454
+00:33:59,780 --> 00:34:06,180
+مجموعة من الأمراض مش مرض واحد وبينتج عنه أن الجسم
+
+455
+00:34:06,180 --> 00:34:11,580
+يصنع خلايا RBCs زيادة عن الطبيعييعني مثلا لو الشخص
+
+456
+00:34:11,580 --> 00:34:14,620
+.. ممكن .. ممكن يكون الشخص نتيجة الظروف اللي بيعيش
+
+457
+00:34:14,620 --> 00:34:18,880
+فيها ممكن يصير عنده polycythemia ممكن نتيجة organ
+
+458
+00:34:18,880 --> 00:34:23,280
+dysfunction يعني مشكلة عنده في أحد أعضاء جسمه فأدت
+
+459
+00:34:23,280 --> 00:34:26,460
+إلى إنه الجسم راح يصنع polycythemia .. يعني راح
+
+460
+00:34:26,460 --> 00:34:30,520
+يصنع RBCs بالزيادة فيكون عنده polycythemia أو حالة
+
+461
+00:34:30,520 --> 00:34:34,360
+ال humor ورم يعني الخلايا بتتكثر بشكل مش طبيعي
+
+462
+00:34:35,110 --> 00:34:38,850
+تمام؟ طيب نبدأ نحكي عنهم واحدة واحدة بالدور ال ..
+
+463
+00:34:38,850 --> 00:34:42,450
+ال .. الظروف اللي بعيش فيها الإنسان الناس اللي
+
+464
+00:34:42,450 --> 00:34:46,070
+بعيشوا على المرتفعات نظرا لو وجود نقص في ال oxygen
+
+465
+00:34:46,070 --> 00:34:50,890
+جسمهم بيصنع خلايا RBCs بزيادة تمام؟ هذه حالة
+
+466
+00:34:50,890 --> 00:34:54,050
+polycythemia صح هم normal بس بيندرجوا تحت ال
+
+467
+00:34:54,050 --> 00:35:01,370
+polycythemia تمام؟و ال .. الناس اللي عندهم organ
+
+468
+00:35:01,370 --> 00:35:05,630
+dysfunction، اذا عند شخص عنده مشكلة بالقلب، القلب
+
+469
+00:35:05,630 --> 00:35:09,690
+هو مضخة الدم، هو اللي بيضخ الدم ل .. ل .. ل سائر
+
+470
+00:35:09,690 --> 00:35:13,630
+أعضاء الجسم، طبعا؟ طيب، فإذا في عندي مشكلة في
+
+471
+00:35:13,630 --> 00:35:16,590
+القلب يعني في عندي مشكلة في ضخ الدم يعني في عندي
+
+472
+00:35:16,590 --> 00:35:21,900
+مشكلة في توصيل ال oxygen، طبعا؟البون مارو اللي
+
+473
+00:35:21,900 --> 00:35:27,340
+بيصنعلي ال .. الدم مش راح يفهم انه المشكلة في ال
+
+474
+00:35:27,340 --> 00:35:33,340
+heart في القلب طبعا؟ لأ هو بس عنده مجسات بيحس نسبة
+
+475
+00:35:33,340 --> 00:35:37,620
+ال oxygen مادام في عندي أي خلل أدى الى نقص نسبة ال
+
+476
+00:35:37,620 --> 00:35:41,340
+oxygen في الدم بيبدأ يصنع RBC's وهو مغمض عيناه
+
+477
+00:35:41,340 --> 00:35:49,620
+طبعا؟ يعني هو مصنع ولا بيسأش زي .. يعني مغم عيناه
+
+478
+00:35:49,990 --> 00:35:54,650
+تمام؟ بيشتغلوا هو مغمض عنه، مش شايف من المشكلة بس
+
+479
+00:35:54,650 --> 00:35:59,900
+حاسس أنه مافيش oxygen، هذا هو البومرةنتيجة مشكلة
+
+480
+00:35:59,900 --> 00:36:02,960
+في القلب، يعني في ضخ الدم أو مشكلة في الرئتين، في
+
+481
+00:36:02,960 --> 00:36:08,540
+التنفس، في هذه الحالتين المحصلة hypoxia، إيش يعني
+
+482
+00:36:08,540 --> 00:36:14,380
+hypoxia؟ hypo قليل، oxia أكسوجين، تمام؟ فبالتالي
+
+483
+00:36:14,380 --> 00:36:19,500
+المحصلة نقص في الأكسوجين في الجسم فال bone مروحة
+
+484
+00:36:19,500 --> 00:36:24,170
+كان مصنع بيشتغل و هو مغمض عيناه، بس بيحسفي .. في
+
+485
+00:36:24,170 --> 00:36:28,890
+Oxygen كافي بيبطل يصنع، مش Oxygen كافي بيصير يصنع،
+
+486
+00:36:28,890 --> 00:36:31,850
+عندي مشكلة في القلب أو في الرئتين، راح تخلي ال
+
+487
+00:36:31,850 --> 00:36:37,270
+bone marrow انه يحس انه مافي Oxygen كافي وبالتالي
+
+488
+00:36:37,270 --> 00:36:42,750
+معناها انه راح يصنع خلايا RBCs بزيادة و المحصلة
+
+489
+00:36:42,750 --> 00:36:47,320
+Polycythemiaالحالة التالتة أو النوع التالت من ال
+
+490
+00:36:47,320 --> 00:36:51,740
+polycythemia أنه يكون في عند تيومر الخلايا الأم لل
+
+491
+00:36:51,740 --> 00:36:55,320
+RBCs اللي موجودة في البون مارو طبعا كل نوع من
+
+492
+00:36:55,320 --> 00:36:59,940
+أنواع خلايا الدم يلو خلايا أم في البون مارو خلايا
+
+493
+00:36:59,940 --> 00:37:05,920
+من .. بس تتكثر بتنتج ليه هذا النوع طبعاالخلاق اللي
+
+494
+00:37:05,920 --> 00:37:09,300
+أم لل RBCs اللي موجودة في ال bone marrow صار فيها
+
+495
+00:37:09,300 --> 00:37:13,380
+خلل طفرة معينة أدت إلى إن هي بتتكاتر بشكل مجنون
+
+496
+00:37:13,380 --> 00:37:17,680
+فبت المحصلة إنه إنتاج المزيد من ال RBCs above
+
+497
+00:37:17,680 --> 00:37:20,740
+normal أكتر من أعلى من ال normal فيعني
+
+498
+00:37:20,740 --> 00:37:24,960
+polycythemiaإذا هي مجموعة من ال disorder و كلهم
+
+499
+00:37:24,960 --> 00:37:28,700
+بندرجوا تحت هذا الإسم، تمام؟ فهي معناه كلمة
+
+500
+00:37:28,700 --> 00:37:31,860
+polycythemia اللي بدي أكم تعرفوا انه hematocrite
+
+501
+00:37:31,860 --> 00:37:37,880
+lower than normal anemia hematocrite above than
+
+502
+00:37:37,880 --> 00:37:42,580
+normal or more than normal polycythemia
+
+503
+00:37:45,160 --> 00:37:49,460
+طيب فال .. اذا هذا هي .. هاي .. هذا التفسير الصورة
+
+504
+00:37:49,460 --> 00:37:54,320
+طيب أول خطوة أو أول نقطة في source of error and
+
+505
+00:37:54,320 --> 00:37:58,540
+comments بيحكيلي عن الimproper sealing يعني لو أنا
+
+506
+00:37:58,540 --> 00:38:01,720
+ما سكرتش كويس ال capability و كل شوية .. من الشوية
+
+507
+00:38:01,720 --> 00:38:05,360
+كنا عم نحكي عنهاحكينا إنه إيش راح يصير؟ راح يصير
+
+508
+00:38:05,360 --> 00:38:09,300
+بيه decrease لل hematocrit Why؟ لإنه في عندي loss
+
+509
+00:38:09,300 --> 00:38:13,660
+لل blood during centrifugation Why؟ لإنه احنا
+
+510
+00:38:13,660 --> 00:38:18,260
+ماسكرناش منيح فأثناء الطرد المركزي صار في عندي طرد
+
+511
+00:38:18,260 --> 00:38:22,620
+للخلايا باتجاه .. باتجاه منطقة مش مسكرة منيح، يعني
+
+512
+00:38:22,620 --> 00:38:27,360
+تثريب لل bloodونظرا لإنه الخلايا ال RBCs هي اللي
+
+513
+00:38:27,360 --> 00:38:32,080
+بتروح بهذا الاتجاه لإنها هي أثقل فاحنا بنفقد RBCs
+
+514
+00:38:32,080 --> 00:38:36,780
+أكتر من ال plasma فطالما أنا بقيس القراءة عندي
+
+515
+00:38:36,780 --> 00:38:39,460
+تبعت هي نسبة ال RBCs إذا راح يكون في عندي
+
+516
+00:38:39,460 --> 00:38:43,140
+decreased hematocritإذن a higher number of
+
+517
+00:38:43,140 --> 00:38:46,980
+erythrocyte are lost in relation to the plasma
+
+518
+00:38:46,980 --> 00:38:51,140
+نسبة ال RBCs اللي أنا بفقدها بالنسبة للبلازمة أكتر
+
+519
+00:38:51,140 --> 00:38:56,300
+لإنها هي الأثقل هي اللي بتتجه باتجاه من .. باتجاه
+
+520
+00:38:56,300 --> 00:38:59,960
+ال .. المنطقة اللي .. اللي المفروض تكون مسكرة إذا
+
+521
+00:38:59,960 --> 00:39:03,320
+مش مسكرة منيح راح يصير فقدان لهذه ال erythrocyte
+
+522
+00:39:03,320 --> 00:39:10,150
+إذا أنا عم بقرأ نسبة و فيها فاقد تمام؟طيب an
+
+523
+00:39:10,150 --> 00:39:13,870
+increased amount of anticoagulant decreased the
+
+524
+00:39:13,870 --> 00:39:16,670
+hematocrit reading as a result of erythrocyte
+
+525
+00:39:16,670 --> 00:39:21,110
+shrinkage ايش معنى الكلام هذا؟ احنا لاحظتوا انه
+
+526
+00:39:21,110 --> 00:39:27,170
+انا ركزت كتير اول المحاضرة على موضوع انه اذا باخد
+
+527
+00:39:27,170 --> 00:39:32,130
+عينة من skin puncture على طول مباشرة بدي اخد
+
+528
+00:39:32,130 --> 00:39:36,050
+hiperionized capillary ال red top او ال red cap
+
+529
+00:39:36,760 --> 00:39:42,240
+capillary إذا أنا باخد عينة من وريد و العينة
+
+530
+00:39:42,240 --> 00:39:45,740
+فرقتها في إيدي تتيوب بعدين بدي أعبي capillary بدي
+
+531
+00:39:45,740 --> 00:39:50,020
+أملأ capillary من هاي ال إيدي تتيوب طبعا فالمفروض
+
+532
+00:39:50,020 --> 00:39:54,880
+انه ال .. ال .. ال capillary هاي تكون plain يعني
+
+533
+00:39:54,880 --> 00:40:01,920
+blue cap او blue top طبعاوحكيتلكم إنه ممنوع أستخدم
+
+534
+00:40:01,920 --> 00:40:06,280
+الهيبرينيز من tube edita ممنوع أمرر البلد على
+
+535
+00:40:06,280 --> 00:40:10,080
+نوعين من ال anticoagulant لإن ال anticoagulants
+
+536
+00:40:10,080 --> 00:40:14,680
+هذه عبارة عن أملاح و الأملاح هاي بتأثر على ال
+
+537
+00:40:14,680 --> 00:40:18,970
+osmolality و ال osmotic pressure ل الخلاياطبعا
+
+538
+00:40:18,970 --> 00:40:23,950
+خلّينا بس نشوف مع بعض هذه الصورة لحتى تتضح الصورة
+
+539
+00:40:23,950 --> 00:40:32,410
+أكتر لحتى تتضح الصورة عندي خلايا دم وجدت في وسط أو
+
+540
+00:40:32,410 --> 00:40:37,090
+في محلول بسميه hypertonic إيش يعني hypertonic؟
+
+541
+00:40:37,090 --> 00:40:41,590
+يعني نسبة الأملح فيها عالية، طبعا؟ يعني blood
+
+542
+00:40:41,590 --> 00:40:46,130
+موجود في إيديتا وهيبرين كمان، نسبة الأملح عالية
+
+543
+00:40:46,130 --> 00:40:51,250
+حوالين الخلايافاحنا عارفين ان ال .. ال water اللي
+
+544
+00:40:51,250 --> 00:40:56,430
+موجود داخل هذه الخلايا .. ال water اللي موجود داخل
+
+545
+00:40:56,430 --> 00:41:02,850
+هذه الخلاياعارفين إن هو بينتقل من الضغط الاسموزي
+
+546
+00:41:02,850 --> 00:41:07,250
+القليل للضغط الاسموزي العالي يعني من تركيز أملح
+
+547
+00:41:07,250 --> 00:41:12,370
+قليل لتركيز أملح عالي فأصبح ال water هذا الماء
+
+548
+00:41:12,370 --> 00:41:16,050
+اللي موجود داخل الخلايا إيش راح يصير فيه؟ راح
+
+549
+00:41:16,050 --> 00:41:21,090
+ينتقل أو يخرج من الخلايا لحتى يحاول إنه يعادل أو
+
+550
+00:41:21,090 --> 00:41:25,130
+يخفف نسبة الأملح اللي موجودة حوالين الخلايا اللي
+
+551
+00:41:25,130 --> 00:41:30,960
+هي ال edit و الhebrine مع بعضطبعا؟ فالمحصلة الخلية
+
+552
+00:41:30,960 --> 00:41:35,520
+لما بتفقد ما إيش بتصير؟ بتكرمش طبعا؟ فبيصير فيها
+
+553
+00:41:35,520 --> 00:41:40,520
+shrinkage بيصير فيها حاجة اسمها shrinkage طبعا؟
+
+554
+00:41:40,520 --> 00:41:45,100
+الخلية لو كان ضغط الأملاح أو ضغط الاسموزي أو نسبة
+
+555
+00:41:45,100 --> 00:41:51,180
+الأملاح حوالين الخلايا مساوية ل .. ال .. ال ..نسبة
+
+556
+00:41:51,180 --> 00:41:56,240
+الأملاح داخل الخلايا إذا الماء هيكون حركته منتظمة
+
+557
+00:41:56,240 --> 00:42:01,080
+من و إلى الخلية إذا الخلية هتبقى intact، سليمة،
+
+558
+00:42:01,080 --> 00:42:04,200
+تمام؟ intact يعني كلمة .. كلمة intact معناها أن
+
+559
+00:42:04,200 --> 00:42:10,330
+الخلايا سليمة، بالشكل السليمتمام لو كان في عندي ال
+
+560
+00:42:10,330 --> 00:42:13,970
+.. ال .. طبعا هذا المحلول اللي بيكون فيه انتظام في
+
+561
+00:42:13,970 --> 00:42:19,610
+نسبة الأملح أو نسبة الأملح في مساوية لتركيزها داخل
+
+562
+00:42:19,610 --> 00:42:27,020
+الخلايا بسمي isotonic solution تمام؟ طيبمحلول نسبة
+
+563
+00:42:27,020 --> 00:42:34,080
+الأملاح فيه أقل من داخل الخلايا بسمي hybotonic إذا
+
+564
+00:42:34,080 --> 00:42:39,060
+الأملاح خارج الخلايا أكتر من داخلها hypertonic
+
+565
+00:42:39,060 --> 00:42:44,340
+أكتر hyper تمام؟ محلول نسبة الأملاح خارج الخلايا
+
+566
+00:42:44,340 --> 00:42:50,260
+مساوية لداخلها بسمي isotonic solutionمحلول نسبة
+
+567
+00:42:50,260 --> 00:42:55,500
+الأملاح فيه أقل من داخل الخلايا أو الوسط يعني
+
+568
+00:42:55,500 --> 00:43:01,140
+الوسط للخلية في نسبة الأملاح أقل من داخلها بسميها
+
+569
+00:43:01,140 --> 00:43:07,260
+hypotonic أقل hypoطيب، مدام نسبة الأملع حوالين
+
+570
+00:43:07,260 --> 00:43:11,060
+الخلايا أقل، معناها كيف هتصير حركة الماء؟ هتصير
+
+571
+00:43:11,060 --> 00:43:14,120
+حركة الماء من التركيز الأقل إلى داخل الخلايا،
+
+572
+00:43:14,120 --> 00:43:18,920
+التركيز الأعلى هيكون هو داخل الخلايا، تمام؟ فإنه
+
+573
+00:43:18,920 --> 00:43:26,720
+نظرا لإن الماء بيتحرك إلى داخل الخلية، معناها إنه
+
+574
+00:43:26,720 --> 00:43:31,320
+ممكن يدخل إلى الخلية ماءأكثر من طاقتها
+
+575
+00:43:31,320 --> 00:43:36,000
+الاستيعابية، مما يؤدي إلى إيش؟ انفجار الخلايا،
+
+576
+00:43:36,000 --> 00:43:42,600
+license للخلايا، تكسر breakdown للخلايا، تمام؟
+
+577
+00:43:42,600 --> 00:43:47,820
+طيب، هاي في عندي كمان صورة تانية لحتى تتضح الفكرة
+
+578
+00:43:47,820 --> 00:43:53,510
+أكترطبعا هاي محلول hypertonic إيش يعني؟ يعني
+
+579
+00:43:53,510 --> 00:43:57,850
+الأملح حوالين الخلايا .. هاي الخلايا اللي لونها
+
+580
+00:43:57,850 --> 00:44:02,470
+أحمر الأملح حوالين الخلايا أكثر من داخلها طبعا
+
+581
+00:44:02,470 --> 00:44:06,170
+أكثر من داخلها شايفين؟ هاي الدائرة هذه .. هاي
+
+582
+00:44:06,170 --> 00:44:09,730
+الدائرة شايفين؟ فيها نسبة أملح اللي لونهم أزرق
+
+583
+00:44:09,730 --> 00:44:14,130
+هدول طبعا أكتر من الأملح اللي موجودة داخل الخلية
+
+584
+00:44:14,130 --> 00:44:23,810
+إيش معناها بدو يصير يا بنات؟معناها بدو ال .. ال ..
+
+585
+00:44:23,810 --> 00:44:28,810
+ايش اللي بدو يصير؟ بدو يصير عندي الشرنكج تكرمش
+
+586
+00:44:28,810 --> 00:44:32,490
+للخلية هذه المصطلحات سيبوكم منها طبعا سيبوكم منها
+
+587
+00:44:32,490 --> 00:44:35,270
+هذه المصطلحات هلأ انا هكتبلكم ايش يعني كلمة
+
+588
+00:44:35,270 --> 00:44:39,790
+shrinkage و ايش يعني intact و .. و .. و licensed
+
+589
+00:44:39,790 --> 00:44:45,370
+بس المهم الفكرة طيب هي هذا الدائرة نسبة الأملح
+
+590
+00:44:45,370 --> 00:44:52,460
+فيهاأقل من اللي داخل الخلية، يعني hypotonic، ماشي،
+
+591
+00:44:52,460 --> 00:44:55,500
+معناها ال .. ال .. الخلية إيش راح يصير؟ راح تنتفخ،
+
+592
+00:44:55,500 --> 00:44:58,960
+تنتفخ، شايفين؟ هي انتفخت، انت تنتفخ، تنتفخ إلى ان
+
+593
+00:44:58,960 --> 00:45:03,540
+يصل فيها الماء أكثر من طاقتها الاستيعابية و بتقوم
+
+594
+00:45:03,540 --> 00:45:09,460
+.. بتنتفجرتمام؟ طيب isotonic يعني تركيز الأملح في
+
+595
+00:45:09,460 --> 00:45:12,960
+.. حوالين الخلايا مساوية تركيزها في الداخل فهي
+
+596
+00:45:12,960 --> 00:45:17,880
+يعني awesome، رائع، wow تمام؟ intact الخلية طيب،
+
+597
+00:45:17,880 --> 00:45:23,760
+خلّينا بس نلخص الاشي عشان أورجيكم ال .. الكلمات
+
+598
+00:45:23,760 --> 00:45:27,520
+يعني لو كانت ال .. ال .. الخلايا موجودة في
+
+599
+00:45:27,520 --> 00:45:31,280
+isotonic تمام؟ solution
+
+600
+00:45:35,280 --> 00:45:41,480
+معناها إيش رح يكون؟ رح تكون intact، سليمة، يعني
+
+601
+00:45:41,480 --> 00:45:46,960
+تركيز الأملح حواليها مساوية تركيز الأملح داخلها،
+
+602
+00:45:46,960 --> 00:45:51,260
+حركة الماء منتظمة من و إلى الخلية، الخلية هتظل
+
+603
+00:45:51,260 --> 00:45:58,060
+intact طيب، لو كان في عندي محلول hypertonic، تمام؟
+
+604
+00:45:58,060 --> 00:46:00,140
+hypertonic solution
+
+605
+00:46:04,350 --> 00:46:08,870
+hypertonic solution إيش معناها؟ معناها تركيز
+
+606
+00:46:08,870 --> 00:46:13,210
+الأملح حوالين الخلايا أكتر من تركيز الأملح داخلها
+
+607
+00:46:13,210 --> 00:46:19,870
+هذا إيش رح يقدي؟ رح يقدي إلى إنه الماء رح يخرج من
+
+608
+00:46:19,870 --> 00:46:25,970
+الخلية إلى خارجها يعني المحص لإن الخلية فقدت سائل
+
+609
+00:46:25,970 --> 00:46:32,510
+فقدت سائل إيش يعني؟ رح يصير فيها shrinkage رح يصير
+
+610
+00:46:32,510 --> 00:46:38,830
+فيها تكرمشطيب لو كان في عندي محلول صار فيه إيه؟ هو
+
+611
+00:46:38,830 --> 00:46:47,670
+عبارة عن hypotonic .. hypotonic solution، تمام؟
+
+612
+00:46:49,230 --> 00:46:53,170
+معناها هذا المحلول أو الوسط المحيط بالخلايا يا
+
+613
+00:46:53,170 --> 00:46:55,910
+بنات سواء محلول أو وسط المحيط يعني نفس المعنى
+
+614
+00:46:55,910 --> 00:46:59,790
+انتوا تخيلوها الوسط المحيط في الخلايا hypotonic
+
+615
+00:46:59,790 --> 00:47:03,850
+يعني تركيز الأملح فيه أقل من تركيزها داخل الخلية
+
+616
+00:47:03,850 --> 00:47:08,030
+طبعا؟ معناها المائش راح ينتقل إلى داخل الخلية لإن
+
+617
+00:47:08,030 --> 00:47:12,400
+التركيز العالي جوا الخليةتمام؟ هذا رح يقدي إلى
+
+618
+00:47:12,400 --> 00:47:17,880
+إيش؟ أول حاجة ال swell ل ال RBCs رح تنتفخ بعدين
+
+619
+00:47:17,880 --> 00:47:22,740
+إلى أن يصل يعني القمر يزيد .. يفيد عن حده و يصير
+
+620
+00:47:22,740 --> 00:47:29,840
+بعديها إيش؟ Lices تمام؟
+
+621
+00:47:29,840 --> 00:47:40,300
+Lices orbreak down تكسر طبعا او خلّيني على كلمة
+
+622
+00:47:40,300 --> 00:47:43,060
+license بلاش يعني break down ممكن تيجي مع ال
+
+623
+00:47:43,060 --> 00:47:46,400
+molecules أكتر من الخلايا فخليها على كلمة license
+
+624
+00:47:46,400 --> 00:47:52,950
+تكسر للخلايا طبعا؟ تحللهذا اللي بيصير في ال .. في
+
+625
+00:47:52,950 --> 00:47:58,290
+الأوساط المختلفة فإذا فكرة أنه ال blood يكون في
+
+626
+00:47:58,290 --> 00:48:02,830
+إيدي تتيوب و أروح أعبيه أو أملأه في hyperionized
+
+627
+00:48:02,830 --> 00:48:08,800
+capillary خطأ hypertonicنوعين من الملح، يعني في
+
+628
+00:48:08,800 --> 00:48:13,220
+عندي hypertonic صار، يعني shrinkage، يعني طالما ..
+
+629
+00:48:13,220 --> 00:48:16,860
+طب إيش يعني shrinkage؟ محصر يعني .. طب أنا قلي
+
+630
+00:48:16,860 --> 00:48:22,800
+ساعة بشرح .. إيش يعني shrinkage؟ معناهاإنه ال
+
+631
+00:48:22,800 --> 00:48:29,440
+RBC's مدام هتكرمشت راح تحتل حجم أصغر، خلايا مكرمشة
+
+632
+00:48:29,440 --> 00:48:33,200
+مش ماخدة الحجم الطبيعي تبعها، معناها الحجم اللي
+
+633
+00:48:33,200 --> 00:48:37,320
+بتحتله أصغر، إذا هيصير في عندي إيش؟ decreased of
+
+634
+00:48:37,320 --> 00:48:40,000
+the hematocrit، تمام؟ راح يصير في عندي decreased
+
+635
+00:48:40,000 --> 00:48:44,290
+of the hematocritفهمتوا يا بنات؟ إيش الفكرة؟ ليش
+
+636
+00:48:44,290 --> 00:48:48,410
+أنا ركزت على إنه أنا بختار الكابلة للصح علشان
+
+637
+00:48:48,410 --> 00:48:52,690
+أشتغل صح، تمام، طيب a decreased or increased
+
+638
+00:48:52,690 --> 00:48:56,210
+result may occur if the specimen was not properly
+
+639
+00:48:56,210 --> 00:49:00,330
+mixed برضه كمان يا بنات إذا أنا ما .. ماعملت
+
+640
+00:49:00,330 --> 00:49:04,450
+mixing منيح للعينة معناها يا إما أنا هقع في خطأ يا
+
+641
+00:49:04,450 --> 00:49:08,370
+إما hypertonic يا hypotonic يعني هي إما هيصير في
+
+642
+00:49:08,370 --> 00:49:11,190
+عند الشرنكش للخلايا فبالتالي decreased hematocrat
+
+643
+00:49:11,370 --> 00:49:15,070
+يعني ممكن يصير swell للخلايا انتفاخ فبيصير ..
+
+644
+00:49:15,070 --> 00:49:18,610
+بيصير increase أو حتى license يعني فقدان للخلايا
+
+645
+00:49:18,610 --> 00:49:24,300
+يعني decrease تمام؟ ده القراءةthe time and speed
+
+646
+00:49:24,300 --> 00:49:28,120
+of the centrifugation and the time when the
+
+647
+00:49:28,120 --> 00:49:30,620
+results are read are very important
+
+648
+00:49:53,150 --> 00:49:58,430
+فلازم يعني طبعا احنا كيف حددوها السرعة هاي و ال ..
+
+649
+00:49:58,430 --> 00:50:02,330
+و الوقت المناسب ل ال centrifugation من خلال عدة
+
+650
+00:50:02,330 --> 00:50:06,230
+دراسات وجدوا انه افضل وقت انه ال five minutes في
+
+651
+00:50:06,230 --> 00:50:08,850
+اعلى سرعة ما بين العشر الف الى خمستاشر الف minute
+
+652
+00:50:09,400 --> 00:50:13,460
+تمام؟ طيب ال insufficient centrifugation لو أنا
+
+653
+00:50:13,460 --> 00:50:17,960
+ماعملتش centrifugation كافى، إيش معناها؟ معناها إن
+
+654
+00:50:17,960 --> 00:50:23,260
+ال RBCs ماترسبتش بشكل كافى، إذا ممكن يكون تحتل حجم
+
+655
+00:50:23,260 --> 00:50:27,330
+أكبر من اللازم، إذا يصير فيه مشكلة في القراءةtime
+
+656
+00:50:27,330 --> 00:50:29,830
+of complete backing should be determined for each
+
+657
+00:50:29,830 --> 00:50:33,390
+centrifuge and rechecked at regular intervals كل
+
+658
+00:50:33,390 --> 00:50:36,270
+centrifuge بيجيني على المختبر يعني اذا انا مثلا
+
+659
+00:50:36,270 --> 00:50:38,990
+عطل عندي centrifuge اشتريت واحد جديد او استلمت
+
+660
+00:50:38,990 --> 00:50:45,990
+واحد جديد لازم انا اجري عدة تجارب لحتى احدد انه
+
+661
+00:50:45,990 --> 00:50:50,470
+يعني اعرف الوقت المناسب والسرعة المناسبة للفصل
+
+662
+00:50:51,060 --> 00:50:55,440
+طبعا؟ من خلال أن أنا مثلا أشتغل عينة عليه و
+
+663
+00:50:55,440 --> 00:51:01,260
+أشتغلها على جهاز آخر و أقارنهم مع بعضطيب the micro
+
+664
+00:51:01,260 --> 00:51:04,240
+hematocrit centrifuge should never be forced to
+
+665
+00:51:04,240 --> 00:51:08,580
+stop حكينا هذا في هذه النقطة واستفدنا حكينا ..
+
+666
+00:51:08,580 --> 00:51:13,220
+حكينا إنه ممنوع أنا أجبر الجهاز إنه يقف أو أوقفه
+
+667
+00:51:13,220 --> 00:51:18,260
+هيك بإيدي أو أحاول أبطق سرعته why؟ لإنه حكينا ال
+
+668
+00:51:18,260 --> 00:51:24,520
+.. ال .. يعني ممنوع أنا أجبره إنه يقف من خلال
+
+669
+00:51:24,520 --> 00:51:27,100
+applying pressure to the metal cover plate عند أنا
+
+670
+00:51:27,100 --> 00:51:32,870
+أحط إيدي أو يعني أعمل ضغط علىال .. الغطى طبعه this
+
+671
+00:51:32,870 --> 00:51:37,030
+will cause ليش حكينا لإنه هيخلى ال RBCs ليه؟ طبقة
+
+672
+00:51:37,030 --> 00:51:41,070
+ال RBCs اللي اترثبت راح يصيرلها sling تنفصل باتجاه
+
+673
+00:51:41,070 --> 00:51:43,810
+ال plasma طبعا راح يصيرلها forward إلى الأمام
+
+674
+00:51:43,810 --> 00:51:47,890
+باتجاه ال plasma و ال result راح ينتج عنها إيه؟
+
+675
+00:51:47,890 --> 00:51:51,530
+انه falsely elevated elevated يعني increased طبعا
+
+676
+00:51:51,530 --> 00:51:56,750
+resultطيب if too much time elapses between when
+
+677
+00:51:56,750 --> 00:51:59,170
+the centrifuge stop and the capillary tube is
+
+678
+00:51:59,170 --> 00:52:04,890
+removed هلأ إذا في وقت طويل مابان توقف ال
+
+679
+00:52:04,890 --> 00:52:08,030
+centrifuge و لما أنا روحت أفضيت و روحت أقرأ
+
+680
+00:52:08,030 --> 00:52:12,110
+النتيجة هذا أيضا ممكن يأثر على النتائج لإنه في هذا
+
+681
+00:52:12,110 --> 00:52:18,630
+الوقت ممكن تكون ال RBCs بدت تحل بدت يعني يبطل
+
+682
+00:52:18,630 --> 00:52:26,290
+ترسيب أو يعني sedimentationتمام فبالتالي ممكن انها
+
+683
+00:52:26,290 --> 00:52:29,990
+تتحرك ايضا باتجاه البلازم وهذا ممكن يعملي falsely
+
+684
+00:52:29,990 --> 00:52:32,010
+elevated hematocrit تمام
+
+685
+00:52:35,890 --> 00:52:39,130
+the buffy coat of a specimen shouldn't be included
+
+686
+00:52:39,130 --> 00:52:43,070
+أنا مش لازم أقرأ و أضمن ال buffy coat من ضمن
+
+687
+00:52:43,070 --> 00:52:45,670
+القراءة حكينا بقرأ من تحت ال .. ال .. ال buffy
+
+688
+00:52:45,670 --> 00:52:47,890
+coat shouldn't be included with the reading
+
+689
+00:52:47,890 --> 00:52:51,750
+because it's inclusion falsely elevated the result
+
+690
+00:52:51,750 --> 00:52:55,570
+يعني القراءة تبعته لو أنا بدي أدخل ال buffy coat
+
+691
+00:52:55,570 --> 00:52:58,890
+في القراءة تبعتي هذا هيرفعلي ال .. ال hematocrit
+
+692
+00:52:58,890 --> 00:53:02,980
+وهذا خطأa decrease or increase in the reading may
+
+693
+00:53:02,980 --> 00:53:06,320
+be seen if the microhematocrit reader is not used
+
+694
+00:53:06,320 --> 00:53:09,840
+properly إذا أنا ما استخدمت التدريج صح ممكن أيضا
+
+695
+00:53:09,840 --> 00:53:12,160
+يكون عندى فيه error في النتيجة decrease or
+
+696
+00:53:12,160 --> 00:53:18,210
+increase تمامركزوا معايا فى النقطة رقم 9 فى عندى
+
+697
+00:53:18,210 --> 00:53:23,870
+أمراض number of disorders عدد من الأمراض زى ال
+
+698
+00:53:23,870 --> 00:53:27,310
+sickles الانيميا انيميا الخلايا المنجالية اللى
+
+699
+00:53:27,310 --> 00:53:30,630
+بيكون فيها الخلايا ال RBC's ماخدة شكل ال .. ال ..
+
+700
+00:53:30,630 --> 00:53:33,810
+ال .. منجل شكل ال .. ال .. ال .. ال .. ال .. ال ..
+
+701
+00:53:33,810 --> 00:53:33,990
+ال .. ال .. ال .. ال .. ال .. ال .. ال .. ال .. ال
+
+702
+00:53:33,990 --> 00:53:34,290
+.. ال .. ال .. ال .. ال .. ال .. ال .. ال .. ال ..
+
+703
+00:53:34,290 --> 00:53:34,630
+ال .. ال .. ال .. ال .. ال .. ال .. ال .. ال .. ال
+
+704
+00:53:34,630 --> 00:53:34,950
+.. ال .. ال .. ال .. ال .. ال .. ال .. ال .. ال ..
+
+705
+00:53:34,950 --> 00:53:36,030
+ال .. ال .. ال .. ال .. ال .. ال .. ال .. ال .. ال
+
+706
+00:53:36,030 --> 00:53:38,790
+.. ال .. ال .. ال .. ال .. ال .. ال .. ال .. ال ..
+
+707
+00:53:38,790 --> 00:53:38,850
+ال .. ال .. ال .. ال .. ال .. ال .. ال .. ال .. ال
+
+708
+00:53:38,850 --> 00:53:42,450
+.. ال .. ال .. ال .. ال .. ال ..
+
+709
+00:53:46,230 --> 00:53:49,470
+hypochromic anemia الخلايا اللى بيكون فيها مشكلة
+
+710
+00:53:49,470 --> 00:53:51,750
+في ال homoglobin يعنى الخلايا بتكون فيها صغيرة
+
+711
+00:53:51,750 --> 00:53:55,930
+اصغر من الطبيعى spherocytosis بتكون الخلايا عاملة
+
+712
+00:53:55,930 --> 00:53:59,570
+زى شكل الكرة مش diskotic و bi concave احنا أخدنا
+
+713
+00:53:59,570 --> 00:54:03,950
+ان ال RBCs إذا طلعت عليها نظرة من فوقبشوفها
+
+714
+00:54:03,950 --> 00:54:09,630
+discotheque، side view بشوفها biconcave، صح؟ طيب،
+
+715
+00:54:09,630 --> 00:54:14,530
+فإذا ال .. ال .. لما أجي أنا أطلع عليها من النظرة
+
+716
+00:54:14,530 --> 00:54:17,870
+الجانبية، بشوفهاش biconcave، بشوفها دائرة، فيعني
+
+717
+00:54:17,870 --> 00:54:23,280
+هذه مخدة شكل الكرة, sphero sideثلاثيميا مرض
+
+718
+00:54:23,280 --> 00:54:27,020
+الانيميا البحر المتوسط وكلنا سمعنا عن الثلاثيميا
+
+719
+00:54:27,020 --> 00:54:32,160
+ومشكلة الثلاثيميا انه بيكون في تباين في شكل ال
+
+720
+00:54:32,160 --> 00:54:39,460
+RBCs طبعا هدولة يبنات الخلل في شكل ال RBCs انيميا
+
+721
+00:54:39,460 --> 00:54:43,340
+خلايا منجلية Sickle Cell انيميا يعني ال RBCs زي
+
+722
+00:54:43,340 --> 00:54:49,570
+شكل الأسشاب macrocytic انيميا خلايا كبيرةوحجم
+
+723
+00:54:49,570 --> 00:54:53,350
+الخلايا كبيرة يعني الخلية نفسها كبيرة hypochromic
+
+724
+00:54:53,350 --> 00:54:57,030
+anemia الخلايا .. الخلية صغيرة spherocytosis
+
+725
+00:54:57,030 --> 00:55:00,330
+الخلية مش مخدة شكل طبيعي مش dystopic ولا biconcave
+
+726
+00:55:00,330 --> 00:55:07,340
+مخدة شكل كروي تمام ثلاثيمياتباين في شكل الخلية هذا
+
+727
+00:55:07,340 --> 00:55:11,860
+رح يقدي أثناء ال .. ال .. الفصل رح يقدي أثناء
+
+728
+00:55:11,860 --> 00:55:15,160
+الفصل أثناء ال centrifugation إلى إنه جزء من اللي
+
+729
+00:55:15,160 --> 00:55:20,800
+بلازمة يصير له trapped ينحجز بين الخلايا وهذا رح
+
+730
+00:55:20,800 --> 00:55:25,300
+يأثر على القراءة تبعتيطيب عشان نفهم الفكرة هذه أنا
+
+731
+00:55:25,300 --> 00:55:31,040
+جبتلكم صورة عبارة عن كورات، تمام؟ خلينا لو نشوف
+
+732
+00:55:31,040 --> 00:55:35,260
+أنا عندي سلة و بدي أصفط فيها الكورات و تكون
+
+733
+00:55:35,260 --> 00:55:40,300
+الكورات بشكل منتظم، تمام؟ و نفس الحجم و نفس الشكل
+
+734
+00:55:40,300 --> 00:55:45,620
+سهل جدا أصفطهابين بعضها، تمام؟ لو جيت هذه الكورات
+
+735
+00:55:45,620 --> 00:55:48,540
+كبيت في .. في داخل السلق أو في داخل ال .. ال .. ال
+
+736
+00:55:48,540 --> 00:55:52,040
+container خلينا نفترض أنها هي داخل صندوق و حطيت
+
+737
+00:55:52,040 --> 00:55:57,400
+صفطتها بشكل منتظم على بعضها البعض و جيت أكب عليها
+
+738
+00:55:57,400 --> 00:56:03,060
+مثلا سائل، مية نسبة الماء اللي ممكن يحتجز داخل أو
+
+739
+00:56:03,060 --> 00:56:06,760
+ما بين الخلايا هتكون قليلة لإنها منتظمة لإن مافيش
+
+740
+00:56:06,760 --> 00:56:15,980
+فراغات ما بينها، تمام؟و نفس الفكرة هنا شايفين؟
+
+741
+00:56:15,980 --> 00:56:21,260
+هذي من لعبة ماخدينها، في لعبة الكورات، تمام؟ بس ان
+
+742
+00:56:21,260 --> 00:56:25,420
+انا أخدوا الفكرة منها، اتخيلي ان هذي ال RBC's
+
+743
+00:56:25,420 --> 00:56:29,060
+تبعتي كلها نفس الشكل و تقضي ان كلها لونها أحمر،
+
+744
+00:56:29,060 --> 00:56:32,910
+يعني كلها red blood cell، تمام؟لو أنا جيت داخل ال
+
+745
+00:56:32,910 --> 00:56:36,450
+cabillary tube و بعد ما عملتي لها centrifugation
+
+746
+00:56:36,450 --> 00:56:39,710
+صار لها sedimentation صفة اتواقع على بعضها بشكل
+
+747
+00:56:39,710 --> 00:56:44,230
+regular تمام؟ بشكل طبعا هيكون المسافات ما بينها
+
+748
+00:56:44,230 --> 00:56:49,410
+قليلة جدا يعني البلازمة يا بنات مش روح تنهجز بينها
+
+749
+00:56:49,410 --> 00:56:55,030
+مش روح تنهجز بينهاتمام؟ فإذا أنا النسبة اللي
+
+750
+00:56:55,030 --> 00:56:59,430
+بقرأها هذه .. نسبة اللي بقرأها .. نسبة الخلايا هي
+
+751
+00:56:59,430 --> 00:57:04,070
+فقط خلايا مش خلايا و plasma صح؟ يعني ال hemotocrit
+
+752
+00:57:04,070 --> 00:57:09,370
+هيكون بيعبرلي بشكل أدق عن نسبة ال RBCs لو كانت
+
+753
+00:57:09,370 --> 00:57:15,080
+الخلايا منتظمة و نفس الشكللكن لو كانت الخلايا منها
+
+754
+00:57:15,080 --> 00:57:20,600
+.. منها زي هيك .. زي هذا الشكل تمام و هي بينها
+
+755
+00:57:20,600 --> 00:57:24,520
+الأسشاب الخلايا اللي .. اللي عاملة .. ماخدة شكل
+
+756
+00:57:24,520 --> 00:57:27,960
+الأسشاب الخلايا المنجلية أو خلايا كبيرة و خلايا
+
+757
+00:57:27,960 --> 00:57:31,120
+صغيرة في ال macrocytic أو خلايا صغيرة و خلايا
+
+758
+00:57:31,120 --> 00:57:35,300
+طبيعية في الhypochromic أو خلايا ماخدة شكل قرصي أو
+
+759
+00:57:35,300 --> 00:57:38,680
+biconcave و خلايا ماخدة شكل كرة أو ان في عندي
+
+760
+00:57:38,680 --> 00:57:45,160
+تباين في أشكال الخلايازي هذا بالظبط خلينا بس نعرض
+
+761
+00:57:45,160 --> 00:57:51,520
+الصورة هاي نشوف افترض ان هدول خلايا RBCs متباينة
+
+762
+00:57:51,520 --> 00:57:55,200
+الشكل إش صغير، إش كبير، إش كروي، إش بيضاوي، إش
+
+763
+00:57:55,200 --> 00:58:00,000
+أسشاب و بدريك تحطيهم في container و تفصليهم مع بعض
+
+764
+00:58:00,000 --> 00:58:04,290
+عشان تاخد الحجمهيكون طبعا مش هيجلسوا على بعض مش
+
+765
+00:58:04,290 --> 00:58:08,010
+هيتصفطوا على بعض بانتظام فبالتالي هيكون في فراغات
+
+766
+00:58:08,010 --> 00:58:12,070
+ما بينهم سهل الفراغات هتتعبيها البلازما إذا أنا
+
+767
+00:58:12,070 --> 00:58:15,230
+لما بدأ أقرأ نسبة الخلايا ال volume اللي بتحتله
+
+768
+00:58:15,230 --> 00:58:21,110
+هذه الخلايا المتباينة هكون بقرأ RBCs وبيتخللها
+
+769
+00:58:21,110 --> 00:58:23,910
+بلازما إذا نسبة ال hemotocrit تبعتي يا بنات
+
+770
+00:58:23,910 --> 00:58:31,090
+إشمالها هتكون خطأنتيجة إيش؟ وجود trapped plasma
+
+771
+00:58:31,090 --> 00:58:35,250
+طبعا، ال plasma to be trapped، ال plasma انحجزت
+
+772
+00:58:35,250 --> 00:58:38,610
+بين الخلايا، الأفضل أن تكون الخلايا منتظمة عشان
+
+773
+00:58:38,610 --> 00:58:41,130
+مايكونش في بينها فرغات و مايقولي plasma ما تصير
+
+774
+00:58:41,130 --> 00:58:46,430
+لها trappingThe trapping of plasma يعني انه ينحجز
+
+775
+00:58:46,430 --> 00:58:50,290
+لبلازما داخل او ما بين في المسافات البينية بين ال
+
+776
+00:58:50,290 --> 00:58:54,650
+RBCs غير منتظمة هذا ممكن يخلي ان ال hematocrit
+
+777
+00:58:54,650 --> 00:58:59,890
+تبعي يكون 1 ل 3% higher than the obtained of the
+
+778
+00:58:59,890 --> 00:59:03,280
+automated instrumentطبعا يعني لو أنا حطيته على
+
+779
+00:59:03,280 --> 00:59:09,060
+جهاز .. جهاز ال CBC و حسبت hematocrit طبعا و قرأته
+
+780
+00:59:09,060 --> 00:59:11,940
+بالتجربة اللي احنا شرحناها بتجربة ال micro
+
+781
+00:59:11,940 --> 00:59:15,240
+hematocrit هلاحظ ان التجربة بتعطيني نسبة
+
+782
+00:59:15,240 --> 00:59:18,520
+hematocrit أعلى من 1 ل 3% يعني لو جهاز ال CBC
+
+783
+00:59:18,520 --> 00:59:25,620
+أعطاني القيمةخمسة و تلاتين تمام على جهاز ال CPC
+
+784
+00:59:25,620 --> 00:59:30,160
+ممكن القراقة باعتى بالاستخدام ال Cabillary Tube
+
+785
+00:59:30,160 --> 00:59:33,100
+تكون ستة و تلاتين أو سبعة و تلاتين أو تمانية و
+
+786
+00:59:33,100 --> 00:59:39,930
+تلاتينطبعا من 1 ل 3% أعلى ليش؟ لإن في نسبة trapped
+
+787
+00:59:39,930 --> 00:59:44,870
+plasma و أنا لما قرأت قرأت ال RBCs الغير منتظمة
+
+788
+00:59:44,870 --> 00:59:49,230
+هذه و ال plasma المحجوزة بينها إذا زادت نسبة ال
+
+789
+00:59:49,230 --> 00:59:52,550
+hematocrit عندي فالأفضل إن الناس اللي عندهم
+
+790
+00:59:52,550 --> 00:59:56,290
+الأمراض هذه ما أشتغللهم ال hematocrit بطريقة ال
+
+791
+00:59:56,290 --> 01:00:01,350
+capillary tubeأشتغله بجهاز ال CBC لإنه جهاز ال CBC
+
+792
+01:00:01,350 --> 01:00:05,290
+بيعمله .. بيعمله calculation و ليس measurement
+
+793
+01:00:05,290 --> 01:00:12,190
+بيحسب ال .. ال hematocrit من ال .. من حجم ال RBC و
+
+794
+01:00:12,190 --> 01:00:17,470
+عددها تمام؟ يعني بيطبق قانون معين و بعدين بي ..
+
+795
+01:00:17,470 --> 01:00:21,690
+بيحسب ليه نسبة ال hematocrit إذا ما بتأثر قيمة ال
+
+796
+01:00:21,690 --> 01:00:24,890
+hematocrit اللي جاية من الجهاز من جهاز ال CBC ما
+
+797
+01:00:24,890 --> 01:00:30,030
+بتتأثر بال trapped plasmaطيب فإذا هذه فكرة trapped
+
+798
+01:00:30,030 --> 01:00:33,310
+plasma الناس اللي عندهم الأمرض هدولة بقرألهم على
+
+799
+01:00:33,310 --> 01:00:36,850
+جهاز ال CBC مابشتغل بطريقة micro hematocrit tube،
+
+800
+01:00:36,850 --> 01:00:40,910
+capillary tube طيب a temporarily low hematocrit
+
+801
+01:00:40,910 --> 01:00:45,490
+reading may result immediately after blood loss لو
+
+802
+01:00:45,490 --> 01:00:51,310
+شخص عنده نزيفسال عنده blood loss، معناها راح يكون
+
+803
+01:00:51,310 --> 01:00:54,530
+في عنده low hematocrit، نتيجة فقدان ال .. ال .. ال
+
+804
+01:00:54,530 --> 01:01:03,140
+blood، نتيجة فقدان ال .. الخلاياأول اشي السبب في
+
+805
+01:01:03,140 --> 01:01:06,200
+ال low hematocrit انه فقد خلايا سفي عنده blood
+
+806
+01:01:06,200 --> 01:01:12,240
+loss نظيف السبب التانى انه الجسم لما بده يعود
+
+807
+01:01:12,240 --> 01:01:17,720
+بيعود ل plasma أسرع مام بعود الخلايا فهيكون في
+
+808
+01:01:17,720 --> 01:01:22,860
+عندى لفترة مؤقتة بعد النظيف حتى لو توقف هيضل انه
+
+809
+01:01:22,860 --> 01:01:26,140
+في عنده لفترة مؤقتة ال hematocrit هيضل له إلى ان
+
+810
+01:01:26,140 --> 01:01:29,300
+الجسم لبين ما ياخد وقته في تعويض ال erythrocyte
+
+811
+01:01:29,300 --> 01:01:35,000
+اللى تم فقدانهاطيب proper specimen collection is
+
+812
+01:01:35,000 --> 01:01:38,240
+important consideration إن أنا أسحب العينة بشكل صح
+
+813
+01:01:38,240 --> 01:01:42,960
+يا بنات برضه كمان بيأثر على النتيجة اتخيلي سحبتي
+
+814
+01:01:42,960 --> 01:01:46,240
+من منطقة فيها hematoma يعني سحبتي hemolized blood
+
+815
+01:01:46,240 --> 01:01:49,880
+blood مكسر يعني ال hematoma هيصير له decreased
+
+816
+01:01:49,880 --> 01:01:56,320
+اتخيلي انت سحبتي ال .. ال .. ال .. ال ..ال tube ..
+
+817
+01:01:56,320 --> 01:02:00,880
+ال blood و مافضطيها كويس فى ال tube فمعناها صار فى
+
+818
+01:02:00,880 --> 01:02:03,860
+clot يعنى فى صار عندى consuming فى ال herbicides
+
+819
+01:02:03,860 --> 01:02:08,860
+معناها هتتأثر النتيجة تخيلي أنه انت سحبت العينة و
+
+820
+01:02:08,860 --> 01:02:12,620
+حطيها فى إيدي تا tube بعدين ملقته hiperionized
+
+821
+01:02:12,880 --> 01:02:15,660
+cabulary معناها صار في عندي shrinkage في الخلايا
+
+822
+01:02:15,660 --> 01:02:19,980
+نتيجة انه في عندي double anticoagulant نوعين من ال
+
+823
+01:02:19,980 --> 01:02:22,380
+anticoagulant معناها يصير فيه decrease في ال
+
+824
+01:02:22,380 --> 01:02:28,020
+hematocrat اذا طريقة جمع العينة و اجراء الفحص مهمة
+
+825
+01:02:28,020 --> 01:02:33,720
+جدا لحتى احصل على representative results او
+
+826
+01:02:33,720 --> 01:02:40,290
+accurate and precise resultنتائج ممثلة معبرة عن
+
+827
+01:02:40,290 --> 01:02:46,950
+حالة الشخص اللى انا عم بشتغله الفحص او يعني نتائج
+
+828
+01:02:46,950 --> 01:02:50,350
+accurate and precise صحيحة و دقيقة the
+
+829
+01:02:50,350 --> 01:02:53,490
+introduction of interstitial fluid from skin لو
+
+830
+01:02:53,490 --> 01:02:57,390
+انا تخيلي ب .. بدي اسحب عينة ال skin puncture
+
+831
+01:02:57,390 --> 01:03:03,090
+ويصيرت اعصر فيها اش يعني؟عصرت فيها حكينا إنه هيصير
+
+832
+01:03:03,090 --> 01:03:07,150
+في عندي tissue juice عصارة الخلايا هتطلع مع ال
+
+833
+01:03:07,150 --> 01:03:11,810
+blood يعني أنا باخد diluted blood مع إني أخدت
+
+834
+01:03:11,810 --> 01:03:15,210
+diluted blood طبيعي إنه نسبة ال RBC هتقل لإنها
+
+835
+01:03:15,210 --> 01:03:22,150
+تخففت يعني decreased hematocrit reading تمام؟طيب
+
+836
+01:03:22,150 --> 01:03:26,370
+أخر نقطة في المحاضرة اللي هي rule of three قانون
+
+837
+01:03:26,370 --> 01:03:30,110
+التلاتة بحكيلك أنه من نسبة ال hematocrit اللي أنت
+
+838
+01:03:30,110 --> 01:03:35,350
+بتحسبيها ممكن تجيبي ال hemoglobin تمام؟ تحسبي نسبة
+
+839
+01:03:35,350 --> 01:03:41,030
+ال hemoglobin يعني قوة الدم تمام؟ بالبلد من القرأة
+
+840
+01:03:41,030 --> 01:03:44,790
+ال hematocritمن خلال تطبيق ال rule of three ولكن
+
+841
+01:03:44,790 --> 01:03:47,850
+هاي القانون يا بنات أنا بس بطبقه عن الناس ال
+
+842
+01:03:47,850 --> 01:03:50,550
+normal هو مابيكونش صالح ان اطبقه عن الناس ال
+
+843
+01:03:50,550 --> 01:03:53,710
+abnormal و اللي عندها مشاكل يعني لو شخص normal
+
+844
+01:03:53,710 --> 01:04:01,150
+طبيعي و كان مثلا ال hematocrit تبعه اتناش ال
+
+845
+01:04:01,150 --> 01:04:06,210
+hematocrit تبعه ستة و تلاتين تمام؟ ستة و تلاتين
+
+846
+01:04:07,920 --> 01:04:12,560
+بحكيلك انه الهموجلبين هو ال hematocrit على تلاتة
+
+847
+01:04:12,560 --> 01:04:18,420
+plus minus تلاتة طبعا خلينا
+
+848
+01:04:18,420 --> 01:04:24,800
+انا اكتب القانون هنا الهموجلبين يا بنات يساوي ال
+
+849
+01:04:24,800 --> 01:04:30,440
+hematocrit على
+
+850
+01:04:30,440 --> 01:04:32,320
+تلاتة
+
+851
+01:04:38,480 --> 01:04:46,500
+اللي هي ماتكرت على تلاتة plus minus plus
+
+852
+01:04:46,500 --> 01:04:52,400
+minus يعني مش عارفة انا اطلعها من ال keyboard plus
+
+853
+01:04:52,400 --> 01:04:59,500
+minus بس انتوا يعني هيها لحظة خليني أجيبها من هنا
+
+854
+01:04:59,500 --> 01:05:04,840
+plus
+
+855
+01:05:04,840 --> 01:05:10,530
+minus تلاتةطبعا لو كان عندي الهيمتوكرت مثلا طلعته
+
+856
+01:05:10,530 --> 01:05:15,010
+36 أعطتكم مثال معناه الهيمتوكرت على تلاتة ستة و
+
+857
+01:05:15,010 --> 01:05:19,670
+تلاتين على تلاتة اتناش طبعا اتناش plus minus تلاتة
+
+858
+01:05:19,670 --> 01:05:23,890
+يعني ال homoglobin بدو يكون من اتناش ناقص تلاتة
+
+859
+01:05:23,890 --> 01:05:29,910
+يعني من تسعة لاتناش plus تلاتة لخمستاش جرام per
+
+860
+01:05:29,910 --> 01:05:33,870
+deciliter هذه واحدة قياس ال homoglobinهذا في حال
+
+861
+01:05:33,870 --> 01:05:37,030
+لو فرضت إن الهموجلبين تلاتة بطلعوا fringe بنات مش
+
+862
+01:05:37,030 --> 01:05:44,970
+قيمة طبعا طيب طب لو أنا بعرف الهموجلبين بس ما بعرف
+
+863
+01:05:44,970 --> 01:05:49,630
+ال hematocrat عادي سهل جدا والله إن ال hematocrat
+
+864
+01:05:49,630 --> 01:05:54,790
+يساوي الهموجلبين
+
+865
+01:05:54,790 --> 01:06:05,850
+ضرب تلاتة plus minus تلاتةلو أنا ب .. بعرف نفرض
+
+866
+01:06:05,850 --> 01:06:11,590
+إنه اللي هم جلبين عشرة plus .. ضرب تلاتة يعني
+
+867
+01:06:11,590 --> 01:06:15,530
+تلاتين plus minus تلاتين يعني من سبعة و عشرين
+
+868
+01:06:15,530 --> 01:06:20,890
+لتلاتة و تلاتين في المية ال hemotocrit طبعا؟ طيب
+
+869
+01:06:20,890 --> 01:06:25,920
+في ممكن انا .. اذا حكينا انا ال hemotocritبعبر عنه
+
+870
+01:06:25,920 --> 01:06:30,520
+بال percentage النسبة المقاوية و ممكن انا ما اعبر
+
+871
+01:06:30,520 --> 01:06:35,820
+عنه بالنسبة المقاوية اعبر عنه بال letter تمام
+
+872
+01:06:35,820 --> 01:06:43,070
+letter bear letter طيب letter bear letterيعني لو
+
+873
+01:06:43,070 --> 01:06:48,350
+كان ال hematocrit 36 في المية كبدأ أحولها لوحدة
+
+874
+01:06:48,350 --> 01:06:52,310
+letter per letter بقسمها عالميا ال 36 بدها تصيراش
+
+875
+01:06:52,310 --> 01:06:57,870
+36 من 100 letter per letter طبعا هذه بنات letter
+
+876
+01:06:57,870 --> 01:07:00,550
+per letter بتروح مع بعضها يعني انا هيك بس حطاها
+
+877
+01:07:00,550 --> 01:07:05,500
+decorطبعا و الفكرة يعني هو بيعبر عنها بال
+
+878
+01:07:05,500 --> 01:07:08,080
+percentage بس انا ممكن يعني اعبر عنها بال letter
+
+879
+01:07:08,080 --> 01:07:11,240
+per letter بس اقسم القيمة على 100 عشان تخلص من
+
+880
+01:07:11,240 --> 01:07:15,420
+النسبة المئوية هذه طبعا اذا عشان لما تشوفوا قيمة
+
+881
+01:07:15,420 --> 01:07:18,920
+ال hemotocrit ماعطيكم مياها بالقيمة هذه سهل جدا
+
+882
+01:07:18,920 --> 01:07:23,050
+تحولوها لنسبة مئوية و تفهموش يعني الكلام هذاهي هنا
+
+883
+01:07:23,050 --> 01:07:27,510
+معطيني انه when specimens are analyzed by either
+
+884
+01:07:27,510 --> 01:07:31,490
+automated or manual method a quick visual check of
+
+885
+01:07:31,490 --> 01:07:34,470
+the result of hemoglobin and hematocrit can be
+
+886
+01:07:34,470 --> 01:07:36,910
+done لو انا اشتغلت ال hemoglobin او ال hematocrit
+
+887
+01:07:36,910 --> 01:07:41,330
+بسواق automated او manual ممكن سهل جدا اطلع عليهم
+
+888
+01:07:41,330 --> 01:07:45,010
+و اشوف هل هم a blind بيطبقوا ال rule of three
+
+889
+01:07:45,010 --> 01:07:48,210
+بيطبقوا قانون التلاتة اللي هو اش بيحكي قانون
+
+890
+01:07:48,210 --> 01:07:54,490
+التلاتة انه ال hemoglobin يساويsorry أنه ال
+
+891
+01:07:54,490 --> 01:07:57,530
+homoglobin بيساوي ال hemotocrit على تلاتة plus
+
+892
+01:07:57,530 --> 01:08:00,690
+minus تلاتة أو ال hemotocrit يساوي ال homoglobin
+
+893
+01:08:00,690 --> 01:08:04,990
+ضارب تلاتة plus minus تلاتة طبعا و هاي هنا يعطيني
+
+894
+01:08:04,990 --> 01:08:13,380
+مثال لو كان عندك أنه ال homoglobin12 طبعا فهي تطبق
+
+895
+01:08:13,380 --> 01:08:16,560
+في قانون ال الهيماتو كريت في القانون هذا هى اللى
+
+896
+01:08:16,560 --> 01:08:23,340
+هو 12 في 3 36 طبعا اذا الهيماتو كريت 36 او هو plus
+
+897
+01:08:23,340 --> 01:08:28,880
+minus 3فالمفروض إنه يقع داخل ال range ما بين تلاتة
+
+898
+01:08:28,880 --> 01:08:33,000
+و تلاتين لتسعة و تلاتين في المية طبعا لو بدي
+
+899
+01:08:33,000 --> 01:08:35,780
+أحولها letter per letter تلاتة و تلاتين من مية
+
+900
+01:08:35,780 --> 01:08:40,240
+لتسعة و تلاتين من مية letter per letter طبعا إذا
+
+901
+01:08:40,240 --> 01:08:44,720
+أحكي ستة و تلاتين في المية أو أحكي point ستة و
+
+902
+01:08:44,720 --> 01:08:48,620
+تلاتين من مية letter per letter نفس المعنىتمام؟
+
+903
+01:08:48,620 --> 01:08:52,620
+هذه طريقتين للتعبير عن قيمة ال hematocrit لذن هيك
+
+904
+01:08:52,620 --> 01:08:56,940
+بنكون وصلنا لنهاية محاضرتنا اليوم ان شاء الله
+
+905
+01:08:56,940 --> 01:09:01,640
+تكونوا استفدتوا واستمتعتوا في المحاضرة و اتمنى ان
+
+906
+01:09:01,640 --> 01:09:05,600
+يكون كل شيء واضح و لو ما كان في شيء واضح او عندكم
+
+907
+01:09:05,600 --> 01:09:10,140
+سؤال او استفسار ما تترددوا تسألوني اما على group
+
+908
+01:09:10,140 --> 01:09:14,380
+ال whats او ان شاء الله من خلال الاجتماع ال meet
+
+909
+01:09:14,380 --> 01:09:17,880
+ال google meet اللي احنا بنعقده اسبوعيايعطيكم
+
+910
+01:09:17,880 --> 01:09:20,240
+العافية وموثقين بإذن الله
+
diff --git a/PL9fwy3NUQKwY9jl8xw-bWkVZkWcGGyspd/OkcVPozKdZ4_raw.json b/PL9fwy3NUQKwY9jl8xw-bWkVZkWcGGyspd/OkcVPozKdZ4_raw.json
new file mode 100644
index 0000000000000000000000000000000000000000..5aa7962dc8b5cb05c47606bb9a20b9eb36224691
--- /dev/null
+++ b/PL9fwy3NUQKwY9jl8xw-bWkVZkWcGGyspd/OkcVPozKdZ4_raw.json
@@ -0,0 +1 @@
+{"segments": [{"id": 1, "seek": 1898, "start": 5.97, "end": 18.99, "text": "السلام عليكم ورحمة الله وبركاته طالبات العزيزات اليوم ان شاء الله راح ناخد مع بعض محاضرة بعنوان glucose test تمام؟ فحص السكر او فحص السكر في الدم او ل glucose test", "tokens": [6027, 3794, 37440, 25894, 24793, 4032, 2288, 35571, 3660, 21984, 4032, 26890, 4117, 9307, 3224, 23032, 6027, 3555, 9307, 18863, 11622, 1829, 11622, 9307, 45595, 20498, 16472, 13412, 16606, 21984, 12602, 39319, 8717, 47283, 3215, 20449, 45030, 11242, 3714, 5016, 46958, 25720, 45030, 1863, 2407, 7649, 23997, 1500, 46811, 10943, 22807, 6156, 5016, 9381, 21136, 37983, 1975, 2407, 6156, 5016, 9381, 21136, 37983, 8978, 32748, 2304, 1975, 2407, 5296, 23997, 1500], "avg_logprob": -0.0934787295344803, "compression_ratio": 1.630952380952381, "no_speech_prob": 1.4841556549072266e-05, "words": [{"start": 5.97, "end": 6.45, "word": "السلام", "probability": 0.90380859375}, {"start": 6.45, "end": 6.85, "word": " عليكم", "probability": 0.9326171875}, {"start": 6.85, "end": 7.21, "word": " ورحمة", "probability": 0.8643798828125}, {"start": 7.21, "end": 7.37, "word": " الله", "probability": 0.97509765625}, {"start": 7.37, "end": 8.03, "word": " وبركاته", "probability": 0.9912109375}, {"start": 8.03, "end": 8.61, "word": " طالبات", "probability": 0.9774169921875}, {"start": 8.61, "end": 9.19, "word": " العزيزات", "probability": 0.98505859375}, {"start": 9.19, "end": 9.47, "word": " اليوم", "probability": 0.87939453125}, {"start": 9.47, "end": 9.59, "word": " ان", "probability": 0.81787109375}, {"start": 9.59, "end": 9.79, "word": " شاء", "probability": 0.9873046875}, {"start": 9.79, "end": 9.95, "word": " الله", "probability": 0.95849609375}, {"start": 9.95, "end": 10.19, "word": " راح", "probability": 0.86669921875}, {"start": 10.19, "end": 10.57, "word": " ناخد", "probability": 0.9747721354166666}, {"start": 10.57, "end": 10.75, "word": " مع", "probability": 0.99560546875}, {"start": 10.75, "end": 11.13, "word": " بعض", "probability": 0.989990234375}, {"start": 11.13, "end": 12.01, "word": " محاضرة", "probability": 0.9681396484375}, {"start": 12.01, "end": 12.67, "word": " بعنوان", "probability": 0.92822265625}, {"start": 12.67, "end": 13.35, "word": " glucose", "probability": 0.9267578125}, {"start": 13.35, "end": 13.75, "word": " test", "probability": 0.896484375}, {"start": 13.75, "end": 14.83, "word": " تمام؟", "probability": 0.6446940104166666}, {"start": 14.83, "end": 15.29, "word": " فحص", "probability": 0.9814453125}, {"start": 15.29, "end": 16.01, "word": " السكر", "probability": 0.936279296875}, {"start": 16.01, "end": 16.31, "word": " او", "probability": 0.7210693359375}, {"start": 16.31, "end": 16.61, "word": " فحص", "probability": 0.9952799479166666}, {"start": 16.61, "end": 17.01, "word": " السكر", "probability": 0.979736328125}, {"start": 17.01, "end": 17.19, "word": " في", "probability": 0.92724609375}, {"start": 17.19, "end": 17.55, "word": " الدم", "probability": 0.98095703125}, 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0.99169921875}, {"start": 419.56, "end": 420.24, "word": " خلال", "probability": 0.99609375}, {"start": 420.24, "end": 420.66, "word": " التلت", "probability": 0.76171875}, {"start": 420.66, "end": 421.04, "word": " شهور", "probability": 0.9969075520833334}, {"start": 421.04, "end": 421.48, "word": " الماضية", "probability": 0.9558919270833334}, {"start": 421.48, "end": 421.82, "word": " خلينا", "probability": 0.721923828125}, {"start": 421.82, "end": 422.18, "word": " نحكي", "probability": 0.9937744140625}, {"start": 422.18, "end": 422.34, "word": " مش", "probability": 0.9833984375}, {"start": 422.34, "end": 422.78, "word": " الأربع", "probability": 0.8663736979166666}, {"start": 422.78, "end": 423.22, "word": " شهور", "probability": 0.9970703125}, {"start": 423.22, "end": 424.0, "word": " تمام؟", "probability": 0.8684895833333334}, {"start": 424.0, "end": 424.12, "word": " ك", "probability": 0.9912109375}, {"start": 424.12, "end": 424.44, "word": " average", "probability": 0.8642578125}, {"start": 424.44, "end": 424.94, "word": " period", "probability": 0.99365234375}, {"start": 424.94, "end": 425.22, "word": " يعني", "probability": 0.959716796875}, {"start": 425.22, "end": 425.66, "word": " كمدة", "probability": 0.9659830729166666}, {"start": 425.66, "end": 426.4, "word": " هيك", "probability": 0.7113037109375}, {"start": 426.4, "end": 427.82, "word": " تقريبية", "probability": 0.923046875}, {"start": 427.82, "end": 428.82, "word": " تمام؟", "probability": 0.9440104166666666}], "temperature": 1.0}, {"id": 19, "seek": 45388, "start": 429.92, "end": 453.88, "text": "والله إذا كان ال homoglobin A1C عالي تمام معناها إنه كان في سكر كتير عالي في الجسم عشان هيك هو ارتبط مع ال homoglobin بشكل كتير كبير و أعطانا تركيز ل homoglobin A1C عالي هذه هي الفكرة إذا homoglobin A1C this test provide an overview for your blood sugar glucose level over a three month time span", "tokens": [2407, 6027, 43761, 11933, 15730, 25961, 2423, 3655, 45841, 13496, 316, 16, 34, 6225, 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"probability": 0.85791015625}, {"start": 436.74, "end": 437.08, "word": " الجسم", "probability": 0.96923828125}, {"start": 437.08, "end": 437.42, "word": " عشان", "probability": 0.9615885416666666}, {"start": 437.42, "end": 437.7, "word": " هيك", "probability": 0.861328125}, {"start": 437.7, "end": 437.82, "word": " هو", "probability": 0.94189453125}, {"start": 437.82, "end": 438.2, "word": " ارتبط", "probability": 0.9493408203125}, {"start": 438.2, "end": 438.34, "word": " مع", "probability": 0.99072265625}, {"start": 438.34, "end": 438.46, "word": " ال", "probability": 0.9697265625}, {"start": 438.46, "end": 439.0, "word": " homoglobin", "probability": 0.9142252604166666}, {"start": 439.0, "end": 439.56, "word": " بشكل", "probability": 0.9933268229166666}, {"start": 439.56, "end": 439.88, "word": " كتير", "probability": 0.9851888020833334}, {"start": 439.88, "end": 440.22, "word": " كبير", "probability": 0.9944661458333334}, {"start": 440.22, "end": 440.38, "word": " و", "probability": 0.923828125}, {"start": 440.38, "end": 440.7, "word": " أعطانا", "probability": 0.850732421875}, {"start": 440.7, "end": 441.04, "word": " تركيز", "probability": 0.989501953125}, {"start": 441.04, "end": 441.12, "word": " ل", "probability": 0.5205078125}, {"start": 441.12, "end": 441.56, "word": " homoglobin", "probability": 0.89111328125}, {"start": 441.56, "end": 442.12, "word": " A1C", "probability": 0.9879557291666666}, {"start": 442.12, "end": 442.54, "word": " عالي", "probability": 0.8938802083333334}, {"start": 442.54, "end": 443.16, "word": " هذه", "probability": 0.48779296875}, {"start": 443.16, "end": 443.36, "word": " هي", "probability": 0.9072265625}, {"start": 443.36, "end": 443.8, "word": " الفكرة", "probability": 0.994140625}, {"start": 443.8, "end": 444.14, "word": " إذا", "probability": 0.52838134765625}, {"start": 444.14, "end": 445.08, "word": " homoglobin", "probability": 0.9083658854166666}, {"start": 445.08, "end": 445.8, "word": " A1C", "probability": 0.9871419270833334}, {"start": 445.8, "end": 446.42, "word": " this", "probability": 0.55029296875}, {"start": 446.42, "end": 446.8, "word": " test", "probability": 0.9033203125}, {"start": 446.8, "end": 447.46, "word": " provide", "probability": 0.70263671875}, {"start": 447.46, "end": 447.82, "word": " an", "probability": 0.85205078125}, {"start": 447.82, "end": 448.54, "word": " overview", "probability": 0.89404296875}, {"start": 448.54, "end": 449.22, "word": " for", "probability": 0.8779296875}, {"start": 449.22, "end": 449.48, "word": " your", "probability": 0.62841796875}, {"start": 449.48, "end": 449.82, "word": " blood", "probability": 0.96630859375}, {"start": 449.82, "end": 450.66, "word": " sugar", "probability": 0.73388671875}, {"start": 450.66, "end": 451.2, "word": " glucose", "probability": 0.8994140625}, {"start": 451.2, "end": 451.8, "word": " level", "probability": 0.94677734375}, {"start": 451.8, "end": 452.52, "word": " over", "probability": 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"end": 456.98, "word": " على", "probability": 0.85693359375}, {"start": 456.98, "end": 457.24, "word": " وضرة", "probability": 0.7633463541666666}, {"start": 457.24, "end": 457.62, "word": " السكر", "probability": 0.912109375}, {"start": 457.62, "end": 457.76, "word": " في", "probability": 0.9609375}, {"start": 457.76, "end": 458.12, "word": " الجسم،", "probability": 0.8470052083333334}, {"start": 458.12, "end": 458.28, "word": " هل", "probability": 0.976318359375}, {"start": 458.28, "end": 458.46, "word": " كان", "probability": 0.9921875}, {"start": 458.46, "end": 458.66, "word": " فيه", "probability": 0.954833984375}, {"start": 458.66, "end": 459.5, "word": " تنظيم،", "probability": 0.93173828125}, {"start": 459.5, "end": 459.68, "word": " هل", "probability": 0.98291015625}, {"start": 459.68, "end": 459.86, "word": " كان", "probability": 0.994140625}, {"start": 459.86, "end": 460.32, "word": " السكر", "probability": 0.955322265625}, {"start": 460.32, "end": 461.08, "word": " طبيعي", "probability": 0.95703125}, {"start": 461.08, "end": 461.38, "word": " خلال", "probability": 0.9871419270833334}, {"start": 461.38, "end": 461.82, "word": " الفترات", "probability": 0.94873046875}, {"start": 461.82, "end": 462.44, "word": " هذه،", "probability": 0.845947265625}, {"start": 462.44, "end": 462.72, "word": " هل", "probability": 0.985107421875}, {"start": 462.72, "end": 462.9, "word": " كان", "probability": 0.9921875}, {"start": 462.9, "end": 463.66, "word": " فيه", "probability": 0.836181640625}, {"start": 463.66, "end": 464.38, "word": " ارتفاع", "probability": 0.9842529296875}, {"start": 464.38, "end": 464.58, "word": " او", "probability": 0.7587890625}, {"start": 464.58, "end": 464.72, "word": " لا", "probability": 0.80517578125}, {"start": 464.72, "end": 466.12, "word": " fasting", "probability": 0.55859375}, {"start": 466.12, "end": 467.02, "word": " blood", "probability": 0.95263671875}, {"start": 467.02, "end": 467.84, "word": " glucose", "probability": 0.9423828125}, {"start": 467.84, "end": 468.6, "word": " fasting", "probability": 0.2080078125}, {"start": 468.6, "end": 469.04, "word": " blood", "probability": 0.98583984375}, {"start": 469.04, "end": 469.74, "word": " glucose", "probability": 0.9716796875}, {"start": 469.74, "end": 470.32, "word": " اللي", "probability": 0.920654296875}, {"start": 470.32, "end": 470.5, "word": " هو", "probability": 0.982421875}, {"start": 470.5, "end": 471.14, "word": " عبارة", "probability": 0.9951171875}, {"start": 471.14, "end": 471.48, "word": " كان", "probability": 0.86865234375}, {"start": 471.48, "end": 471.86, "word": " زمان", "probability": 0.990234375}, {"start": 471.86, "end": 472.32, "word": " نسميه", "probability": 0.942138671875}, {"start": 472.32, "end": 472.46, "word": " أو", "probability": 0.5859375}, {"start": 472.46, "end": 472.98, "word": " لساتنا", "probability": 0.9765625}, {"start": 472.98, "end": 473.22, "word": " يعني", "probability": 0.751220703125}, {"start": 473.22, "end": 474.0, "word": " لازال", "probability": 0.9386393229166666}, {"start": 474.0, "end": 474.36, "word": " الاسم", "probability": 0.9060872395833334}, {"start": 474.36, "end": 474.8, "word": " دارش", "probability": 0.89599609375}, {"start": 474.8, "end": 475.24, "word": " fasting", "probability": 0.83154296875}, {"start": 475.24, "end": 475.62, "word": " blood", "probability": 0.9716796875}, {"start": 475.62, "end": 475.94, "word": " sugar", "probability": 0.86474609375}], "temperature": 1.0}, {"id": 21, "seek": 50288, "start": 476.96, "end": 502.88, "text": "تمام؟ سواء fasting blood glucose أو fasting blood sugar هو نفس المعنى هو الفحص اللي احنا بنقيز فيه مستوى السكر في الدم لشخص صايم تمام؟ على الأقل تمان ساعات احنا زمان او في بداية المساق يعني مش زمان كتير انه حكينا انه كل فحص له شروط وحكيتلكم انه احيانا بيتطلب الفحص ان الشخص يكون صايم تمان ساعات مثلا", "tokens": [39237, 10943, 22807, 8608, 2407, 16606, 22371, 3390, 23997, 34051, 22371, 3390, 5076, 31439, 8717, 36178, 9673, 3615, 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{"start": 477.78, "end": 478.2, "word": " سواء", "probability": 0.7227783203125}, {"start": 478.2, "end": 478.6, "word": " fasting", "probability": 0.974609375}, {"start": 478.6, "end": 479.0, "word": " blood", "probability": 0.97509765625}, {"start": 479.0, "end": 479.58, "word": " glucose", "probability": 0.9638671875}, {"start": 479.58, "end": 479.76, "word": " أو", "probability": 0.80322265625}, {"start": 479.76, "end": 480.14, "word": " fasting", "probability": 0.9892578125}, {"start": 480.14, "end": 480.52, "word": " blood", "probability": 0.9794921875}, {"start": 480.52, "end": 480.84, "word": " sugar", "probability": 0.8720703125}, {"start": 480.84, "end": 481.04, "word": " هو", "probability": 0.9560546875}, {"start": 481.04, "end": 481.34, "word": " نفس", "probability": 0.9921875}, {"start": 481.34, "end": 481.88, "word": " المعنى", "probability": 0.986328125}, {"start": 481.88, "end": 482.34, "word": " هو", "probability": 0.67529296875}, {"start": 482.34, "end": 482.88, 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{"start": 905.43, "end": 905.97, "word": " فينول", "probability": 0.6918131510416666}, {"start": 905.97, "end": 906.47, "word": " في", "probability": 0.58984375}, {"start": 906.47, "end": 906.87, "word": " وجود", "probability": 0.990478515625}, {"start": 906.87, "end": 907.49, "word": " أنزايم", "probability": 0.7779541015625}, {"start": 907.49, "end": 907.63, "word": " اللي", "probability": 0.975830078125}, {"start": 907.63, "end": 907.81, "word": " هو", "probability": 0.986328125}, {"start": 907.81, "end": 908.79, "word": " peroxidease", "probability": 0.5869140625}], "temperature": 1.0}, {"id": 42, "seek": 92489, "start": 909.21, "end": 924.89, "text": "هذا طبعا ال amino phenazone هو عبارة عن oxygen receptor هياخد oxygen من ال peroxide و يتحد مع الفينول و يعطيهم quinamine و هذا ال quinamine اللي هو عبارة عن colored complex لونه pink لونه زهري تمام؟", "tokens": [3224, 15730, 23032, 3555, 3615, 995, 2423, 24674, 7279, 921, 546, 31439, 6225, 3555, 9640, 3660, 18871, 9169, 32264, 39896, 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مثلا وجود الخلايا هتستهلك السكر فهتأثرلي على النتيجة انا مش هقيز كل مستوى السكر ساعتها لإن في جزء تماشي استهلاكه بواسطة الخلايا فلأ يفضل فصل الملازمة عن الخلايا على طول فورا بعد سحب العينة أفضل", "tokens": [5172, 15042, 4724, 16254, 6225, 10943, 1211, 19753, 34740, 7251, 1829, 7435, 1829, 7251, 10721, 49115, 15844, 28239, 31371, 7435, 3660, 30767, 1829, 50113, 15040, 49610, 23328, 33962, 15040, 25528, 8032, 2655, 14851, 3224, 23275, 21136, 37983, 6156, 3224, 2655, 10721, 49115, 20292, 15844, 28239, 31371, 7435, 3660, 1975, 8315, 37893, 8032, 38436, 11622, 28242, 3714, 14851, 2407, 7578, 21136, 37983, 8608, 995, 34268, 11296, 5296, 28814, 1863, 8978, 10874, 11622, 38207, 46811, 33599, 1829, 44713, 3224, 15040, 4117, 3224, 4724, 14407, 3794, 9566, 3660, 33962, 15040, 25528, 6156, 1211, 10721, 7251, 5172, 11242, 1211, 6156, 36520, 9673, 1211, 31377, 46007, 18871, 33962, 15040, 25528, 15844, 23032, 12610, 6156, 13063, 995, 39182, 8608, 5016, 3555, 18863, 9957, 3660, 5551, 5172, 11242, 1211], "avg_logprob": -0.10480183181239337, "compression_ratio": 1.90625, "no_speech_prob": 1.0132789611816406e-06, "words": [{"start": 1230.45, "end": 1230.73, "word": "فما", "probability": 0.96923828125}, {"start": 1230.73, "end": 1231.13, "word": " بدي", "probability": 0.809814453125}, {"start": 1231.13, "end": 1231.61, "word": " عامل", "probability": 0.9978841145833334}, {"start": 1231.61, "end": 1232.01, "word": " آخر", "probability": 0.787353515625}, {"start": 1232.01, "end": 1232.33, "word": " ييجي", "probability": 0.9024658203125}, {"start": 1232.33, "end": 1232.71, "word": " يأثر", "probability": 0.8610026041666666}, {"start": 1232.71, "end": 1232.97, "word": " على", "probability": 0.88916015625}, {"start": 1232.97, "end": 1233.53, "word": " النتيجة", "probability": 0.9639892578125}, {"start": 1233.53, "end": 1233.73, "word": " زي", "probability": 0.723388671875}, {"start": 1233.73, "end": 1234.01, "word": " مثلا", "probability": 0.954833984375}, {"start": 1234.01, "end": 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"end": 1240.37, "word": " السكر", "probability": 0.97216796875}, {"start": 1240.37, "end": 1240.99, "word": " ساعتها", "probability": 0.995849609375}, {"start": 1240.99, "end": 1241.63, "word": " لإن", "probability": 0.9124348958333334}, {"start": 1241.63, "end": 1241.81, "word": " في", "probability": 0.42431640625}, {"start": 1241.81, "end": 1242.29, "word": " جزء", "probability": 0.9840494791666666}, {"start": 1242.29, "end": 1243.45, "word": " تماشي", "probability": 0.78515625}, {"start": 1243.45, "end": 1244.11, "word": " استهلاكه", "probability": 0.9716796875}, {"start": 1244.11, "end": 1244.69, "word": " بواسطة", "probability": 0.941015625}, {"start": 1244.69, "end": 1245.29, "word": " الخلايا", "probability": 0.9793294270833334}, {"start": 1245.29, "end": 1245.95, "word": " فلأ", "probability": 0.95947265625}, {"start": 1245.95, "end": 1246.69, "word": " يفضل", "probability": 0.9857177734375}, {"start": 1246.69, "end": 1247.15, "word": " فصل", "probability": 0.9091796875}, {"start": 1247.15, "end": 1247.95, "word": " الملازمة", "probability": 0.7896728515625}, {"start": 1247.95, "end": 1248.19, "word": " عن", "probability": 0.99267578125}, {"start": 1248.19, "end": 1248.95, "word": " الخلايا", "probability": 0.9742838541666666}, {"start": 1248.95, "end": 1249.99, "word": " على", "probability": 0.529296875}, {"start": 1249.99, "end": 1250.33, "word": " طول", "probability": 0.9921875}, {"start": 1250.33, "end": 1250.87, "word": " فورا", "probability": 0.9583333333333334}, {"start": 1250.87, "end": 1251.15, "word": " بعد", "probability": 0.9775390625}, {"start": 1251.15, "end": 1251.57, "word": " سحب", "probability": 0.9716796875}, {"start": 1251.57, "end": 1252.35, "word": " العينة", "probability": 0.87890625}, {"start": 1252.35, "end": 1253.65, "word": " أفضل", "probability": 0.948974609375}], "temperature": 1.0}, {"id": 58, "seek": 126868, "start": 1254.52, "end": 1268.68, "text": "أو خلال نص ساعة طيب التجربة تبعتي إيش بدني أعمل؟ بدي أجيب تلت قنابيد و أعلمهم انبوبة بسميها blank انبوبة بسميها standard و انبوبة بسميها ال sample أو ال test", "tokens": [10721, 2407, 16490, 1211, 6027, 8717, 9381, 8608, 995, 27884, 23032, 1829, 3555, 16712, 7435, 25513, 3660, 6055, 3555, 3615, 31371, 11933, 1829, 8592, 47525, 22653, 5551, 25957, 1211, 22807, 4724, 16254, 5551, 7435, 1829, 3555, 6055, 1211, 2655, 12174, 8315, 21292, 3215, 4032, 5551, 3615, 19528, 16095, 16472, 3555, 37746, 3660, 4724, 38251, 1829, 11296, 8247, 16472, 3555, 37746, 3660, 4724, 38251, 1829, 11296, 3832, 4032, 16472, 3555, 37746, 3660, 4724, 38251, 1829, 11296, 2423, 6889, 34051, 2423, 1500], "avg_logprob": -0.11622299382716049, "compression_ratio": 1.6477987421383649, "no_speech_prob": 4.291534423828125e-06, "words": [{"start": 1254.52, "end": 1254.8, "word": "أو", "probability": 0.821533203125}, {"start": 1254.8, "end": 1255.14, "word": " خلال", "probability": 0.99755859375}, {"start": 1255.14, "end": 1255.4, "word": " نص", "probability": 0.970703125}, {"start": 1255.4, 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1260.12, "end": 1260.96, "word": " أعلمهم", "probability": 0.9454345703125}, {"start": 1260.96, "end": 1261.74, "word": " انبوبة", "probability": 0.82611083984375}, {"start": 1261.74, "end": 1262.24, "word": " بسميها", "probability": 0.9857177734375}, {"start": 1262.24, "end": 1262.8, "word": " blank", "probability": 0.6552734375}, {"start": 1262.8, "end": 1264.0, "word": " انبوبة", "probability": 0.9183349609375}, {"start": 1264.0, "end": 1264.56, "word": " بسميها", "probability": 0.99462890625}, {"start": 1264.56, "end": 1265.3, "word": " standard", "probability": 0.95947265625}, {"start": 1265.3, "end": 1266.1, "word": " و", "probability": 0.9794921875}, {"start": 1266.1, "end": 1266.78, "word": " انبوبة", "probability": 0.9486083984375}, {"start": 1266.78, "end": 1267.32, "word": " بسميها", "probability": 0.995361328125}, {"start": 1267.32, "end": 1267.56, "word": " ال", "probability": 0.841796875}, {"start": 1267.56, "end": 1267.92, "word": " sample", "probability": 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"probability": 0.83837890625}, {"start": 1278.14, "end": 1278.78, "word": " standard", "probability": 0.97119140625}, {"start": 1278.78, "end": 1279.38, "word": " ولا", "probability": 0.9453125}, {"start": 1279.38, "end": 1279.94, "word": " sample", "probability": 0.9443359375}, {"start": 1279.94, "end": 1281.6, "word": " طيب", "probability": 0.9488932291666666}, {"start": 1281.6, "end": 1283.42, "word": " التقيوب", "probability": 0.5654296875}, {"start": 1283.42, "end": 1283.58, "word": " اللي", "probability": 0.7783203125}, {"start": 1283.58, "end": 1283.86, "word": " فيها", "probability": 0.9833984375}, {"start": 1283.86, "end": 1284.44, "word": " standard", "probability": 0.9736328125}, {"start": 1284.44, "end": 1284.78, "word": " فيها", "probability": 0.964111328125}, {"start": 1284.78, "end": 1284.88, "word": " ال", "probability": 0.87939453125}, {"start": 1284.88, "end": 1285.48, "word": " reagent", "probability": 0.988037109375}, {"start": 1285.48, "end": 1285.72, "word": " مع", "probability": 0.98974609375}, {"start": 1285.72, "end": 1286.52, "word": " مين؟", "probability": 0.9313151041666666}, {"start": 1286.52, "end": 1286.92, "word": " مع", "probability": 0.97705078125}, {"start": 1286.92, "end": 1287.52, "word": " ال", "probability": 0.970703125}, {"start": 1287.52, "end": 1288.0, "word": " standard", "probability": 0.97802734375}], "temperature": 1.0}, {"id": 60, "seek": 130268, "start": 1289.02, "end": 1302.68, "text": "والتيوب اللي فيها ال sample هيكون فيها ال reagent مع ال sample، اذا التيوب ال standard هيكون فيها reagent و standard وتيوب ال sample هيكون فيها ال reagent و ال sample أو ال specimen", "tokens": [2407, 6027, 31371, 37746, 13672, 1829, 8978, 11296, 2423, 6889, 39896, 30544, 8978, 11296, 2423, 26949, 317, 20449, 2423, 6889, 12399, 1975, 15730, 2423, 31371, 37746, 2423, 3832, 39896, 30544, 8978, 11296, 26949, 317, 4032, 3832, 4032, 31371, 37746, 2423, 6889, 39896, 30544, 8978, 11296, 2423, 26949, 317, 4032, 2423, 6889, 34051, 2423, 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"word": " إيش", "probability": 0.659912109375}, {"start": 1911.38, "end": 1911.9, "word": " استهلاك", "probability": 0.7911376953125}, {"start": 1911.9, "end": 1912.22, "word": " طاقة", "probability": 0.9718424479166666}], "temperature": 1.0}, {"id": 87, "seek": 192069, "start": 1913.59, "end": 1920.69, "text": "هيك بنكون وصلنا لنهاية محاضرتنا لليوم أتمنى أنها تكون بسيطة وواضحة لإلكم ويعطيكم ألف عافية", "tokens": [3224, 1829, 4117, 44945, 30544, 4032, 36520, 8315, 5296, 1863, 11296, 10632, 3714, 5016, 46958, 43500, 8315, 5296, 20292, 20498, 5551, 39237, 1863, 7578, 14739, 11296, 6055, 30544, 4724, 3794, 1829, 9566, 3660, 4032, 2407, 46958, 5016, 3660, 5296, 28814, 23275, 2304, 4032, 40228, 9566, 1829, 24793, 5551, 46538, 6225, 31845, 10632], "avg_logprob": -0.12138856019613878, "compression_ratio": 1.4310344827586208, "no_speech_prob": 5.960464477539063e-08, "words": [{"start": 1913.59, "end": 1913.93, "word": "هيك", "probability": 0.7993977864583334}, {"start": 1913.93, "end": 1914.51, "word": " بنكون", "probability": 0.983154296875}, {"start": 1914.51, "end": 1915.15, "word": " وصلنا", "probability": 0.9864908854166666}, {"start": 1915.15, "end": 1915.71, "word": " لنهاية", "probability": 0.9495849609375}, {"start": 1915.71, "end": 1916.39, "word": " محاضرتنا", "probability": 0.94443359375}, {"start": 1916.39, "end": 1916.83, "word": " لليوم", "probability": 0.9114583333333334}, {"start": 1916.83, "end": 1917.39, "word": " أتمنى", "probability": 0.73394775390625}, {"start": 1917.39, "end": 1917.57, "word": " أنها", "probability": 0.72509765625}, {"start": 1917.57, "end": 1917.91, "word": " تكون", "probability": 0.992919921875}, {"start": 1917.91, "end": 1918.49, "word": " بسيطة", "probability": 0.9908203125}, {"start": 1918.49, "end": 1919.05, "word": " وواضحة", "probability": 0.9033203125}, {"start": 1919.05, "end": 1919.49, "word": " لإلكم", "probability": 0.8902587890625}, {"start": 1919.49, "end": 1919.95, "word": " ويعطيكم", "probability": 0.88515625}, {"start": 1919.95, "end": 1920.21, "word": " ألف", "probability": 0.797119140625}, {"start": 1920.21, "end": 1920.69, "word": " عافية", "probability": 0.9541015625}], "temperature": 1.0}], "language": "ar", "language_probability": 1.0, "duration": 1925.189625, "duration_after_vad": 1897.318749999998}
\ No newline at end of file
diff --git a/PL9fwy3NUQKwY9jl8xw-bWkVZkWcGGyspd/OkcVPozKdZ4_raw.srt b/PL9fwy3NUQKwY9jl8xw-bWkVZkWcGGyspd/OkcVPozKdZ4_raw.srt
new file mode 100644
index 0000000000000000000000000000000000000000..ef8b293bdc3be9f516e220733d8802ecf9506f4a
--- /dev/null
+++ b/PL9fwy3NUQKwY9jl8xw-bWkVZkWcGGyspd/OkcVPozKdZ4_raw.srt
@@ -0,0 +1,1728 @@
+1
+00:00:05,970 --> 00:00:09,190
+السلام عليكم ورحمة الله وبركاته طالبات العزيزات
+
+2
+00:00:09,190 --> 00:00:12,670
+اليوم ان شاء الله راح ناخد مع بعض محاضرة بعنوان
+
+3
+00:00:12,670 --> 00:00:17,550
+glucose test تمام؟ فحص السكر او فحص السكر في الدم
+
+4
+00:00:17,550 --> 00:00:22,900
+او ل glucose testفي البداية blood glucose is the
+
+5
+00:00:22,900 --> 00:00:26,140
+main sugar that the body made from the food in the
+
+6
+00:00:26,140 --> 00:00:31,440
+diet فعليا احنا بنتناوأ الأغذية، هذه الأغذية ممكن
+
+7
+00:00:31,440 --> 00:00:36,280
+تكون سكريات، ممكن تكون carbohydrate معقدة، نشويات
+
+8
+00:00:36,280 --> 00:00:42,220
+مثلا وما شابه، ممكن تكون بروتينات أو دهونلابد من
+
+9
+00:00:42,220 --> 00:00:46,760
+تحويل هذه المواد الغذائية إلى سكر في الدم، تمام؟ و
+
+10
+00:00:46,760 --> 00:00:51,200
+هذا السكر هو ال glucose، تمام؟ لازم يكون glucose
+
+11
+00:00:51,200 --> 00:00:56,400
+لحتى الخلايا تستطيع انها تاخد هذا ال glucose و
+
+12
+00:00:56,400 --> 00:01:00,940
+تصنع منه طاقة، اذا ال blood glucose هو .. يعني
+
+13
+00:01:00,940 --> 00:01:05,370
+السكر الرئيسي اللي احنا بنشوفه في ال ..في الدم أو
+
+14
+00:01:05,370 --> 00:01:10,310
+في الجسم نظرا لإن هو النوع السكري الوحيد اللي ممكن
+
+15
+00:01:10,310 --> 00:01:15,710
+تعرف الخلايا تتعامل معاه تاخدوا تدخلوا اليها عبر
+
+16
+00:01:15,710 --> 00:01:21,430
+مستقبل على سطحها و تصنع من خلاله الطاقة تمام؟
+
+17
+00:01:21,430 --> 00:01:26,600
+produce energyglucose is a simple sugar هو عبارة
+
+18
+00:01:26,600 --> 00:01:30,900
+عن سكر بسيط، monosaccharide، سكر أحادي، the body
+
+19
+00:01:30,900 --> 00:01:35,480
+produce it from protein, fat and in largest part
+
+20
+00:01:35,480 --> 00:01:41,040
+carbohydrate الجسم بيصنعه بشكل أساسي أو بشكل كتير
+
+21
+00:01:41,040 --> 00:01:44,620
+كبير من ال carbohydrate إلى أنوقات أيضا يتم تصنيعه
+
+22
+00:01:44,620 --> 00:01:48,820
+من البروتينات و الدهون مثل ما ذكرنا سابقا
+
+23
+00:01:49,380 --> 00:01:52,820
+الجليجلوكوز is carried through the blood stream to
+
+24
+00:01:52,820 --> 00:01:55,820
+provide energy to all cells in the body اذا ايش
+
+25
+00:01:55,820 --> 00:02:00,260
+وظيفة الجليجلوكوز او ايش اهمته ان هو ينقل عبارة دم
+
+26
+00:02:00,260 --> 00:02:05,040
+الى الخلايا الخلايا بتعمله uptake بتاخده و بتصنع
+
+27
+00:02:05,040 --> 00:02:08,600
+من خلاله الطاقة من خلال عمليات ال glycolysis و
+
+28
+00:02:08,600 --> 00:02:12,460
+creep cycle و ال energy production metabolism اللي
+
+29
+00:02:12,460 --> 00:02:18,420
+انتوا تعلمتوها في ال biochemengusted glucose ال ..
+
+30
+00:02:18,420 --> 00:02:22,820
+ال .. ال glucose اللي يتم تناولوه عبر الفم،
+
+31
+00:02:22,820 --> 00:02:26,740
+engusted glucose is absorbed directly into the
+
+32
+00:02:26,740 --> 00:02:29,780
+blood from the intestine and result in rapid
+
+33
+00:02:29,780 --> 00:02:33,200
+increase in blood glucose. هلأ إذا احنا تناولنا
+
+34
+00:02:33,200 --> 00:02:38,050
+glucose تمامهذا ال glucose يتم امتصاصه مباشرة من
+
+35
+00:02:38,050 --> 00:02:41,710
+ال intestine و على طول بيفوت الدم مابيحتاجش عملية
+
+36
+00:02:41,710 --> 00:02:45,230
+تحويل مش زي السكريات المعقدة أو السكريات اللي هي
+
+37
+00:02:45,230 --> 00:02:48,150
+بنسميها مثلا disaccharide أو polysaccharide أو
+
+38
+00:02:48,150 --> 00:02:52,430
+starch أو whatever فهدول لأ لازم يمروا بمراحل
+
+39
+00:02:52,430 --> 00:02:57,810
+تكسير وتحويل علشان يتحولوا لglucose بعدين يعني يتم
+
+40
+00:02:57,810 --> 00:03:01,920
+نقلهم عبر الدم إلى الخلايالكن اذا احنا عملنا
+
+41
+00:03:01,920 --> 00:03:06,940
+ingested glucose يعني تناولنا glucose pure فهذا
+
+42
+00:03:06,940 --> 00:03:10,260
+على طول يتم امتصاصه من ال intestine بيفوت الدم و
+
+43
+00:03:10,260 --> 00:03:14,640
+بتنقله الى الخلايا الخلايا طبعا يا اما تستخدمه
+
+44
+00:03:14,640 --> 00:03:19,800
+كمصدر للطاقة اذا هي محتاجة طاقة او راح اتخزنهفإذا
+
+45
+00:03:19,800 --> 00:03:22,720
+ال cells can't use glucose without the help of
+
+46
+00:03:22,720 --> 00:03:26,800
+insulin طبعا بعد إحنا ما نتناول ال glucose و يتم
+
+47
+00:03:26,800 --> 00:03:31,480
+امتصاصه من ال intestine تمام و يفوت على الدم هذا
+
+48
+00:03:31,480 --> 00:03:36,500
+رح يؤدي إلى أو ينتج عنه rapid increase in blood
+
+49
+00:03:36,500 --> 00:03:42,600
+glucose هذا رح يزود أو يعلي تركيز ال glucose في
+
+50
+00:03:42,600 --> 00:03:46,570
+الدمcells can't use glucose without the help of
+
+51
+00:03:46,570 --> 00:03:51,610
+insulin طبعا الخلايا ما بتستطيع انها تصنع او انه
+
+52
+00:03:51,610 --> 00:03:56,650
+تستخدم ال glucose او تصنع منه طاقة بدون مساعدة ال
+
+53
+00:03:56,650 --> 00:04:00,250
+insulin اذا ال insulin هو المفتاح اللي بيفتح بوابة
+
+54
+00:04:00,250 --> 00:04:04,130
+ال glucose و بيخلي الخلايا مثلا اتمرر ال glucose
+
+55
+00:04:04,130 --> 00:04:09,030
+الى داخلها وبالتالي يمكن بعد هيك تصنيع منه طاقة
+
+56
+00:04:09,030 --> 00:04:13,630
+اذا كنا محتاجين energy او تخزينهالجلوكوز إيه له
+
+57
+00:04:13,630 --> 00:04:17,330
+اسم آخر اللي هو dextrose، تمام؟ إيه له اسم آخر؟
+
+58
+00:04:17,330 --> 00:04:23,770
+Dextrose، في عندي عدة فحوصات احنا ب .. ممكن نجريها
+
+59
+00:04:23,770 --> 00:04:28,630
+على عينة المريض و هي مرتبطة بشكل ما بال blood
+
+60
+00:04:28,630 --> 00:04:33,860
+glucose testهي عدة أشكال لتقييم موضع السكر في الدم
+
+61
+00:04:33,860 --> 00:04:38,740
+من هذه الفحصات ال homoglobin A1C الفحص الأول
+
+62
+00:04:38,740 --> 00:04:42,240
+homoglobin A1C هلأ ال .. ال .. ال .. احنا كلنا
+
+63
+00:04:42,240 --> 00:04:45,520
+عارفين انه ال glucose بيفوت على الخلايا إحدى
+
+64
+00:04:45,520 --> 00:04:49,040
+الخلايا اللي بيفوت عليها ال glucose هي ال RBCs
+
+65
+00:04:49,040 --> 00:04:53,910
+خلايا الدم الحمرعفهناك جزء منه بيرتبط مع ال
+
+66
+00:04:53,910 --> 00:04:57,450
+homoglobin و بنسمي ال homoglobin المرتبط مع ال
+
+67
+00:04:57,450 --> 00:05:04,090
+glucose-glycated homoglobin أو homoglobin A1C طبعا
+
+68
+00:05:04,090 --> 00:05:09,210
+إذا كان في عندي زيادة في نسبة السكر في الدم هذا رح
+
+69
+00:05:09,210 --> 00:05:15,470
+يؤدي إلى زيادة في ارتباط السكر مع ال homoglobin A1
+
+70
+00:05:15,470 --> 00:05:19,130
+.. ال homoglobin اللي موجود في ال RBCs فبالتالي رح
+
+71
+00:05:19,130 --> 00:05:24,880
+يؤدي إلى زيادةفي تركيز ال homoglobin A1C ونظرا
+
+72
+00:05:24,880 --> 00:05:31,960
+لإنه ال RBCs يعني ال average لحياتها معدل حياتها
+
+73
+00:05:31,960 --> 00:05:37,320
+او معدل ال life span تبعتها 120 يوم اذا انا ب .. ب
+
+74
+00:05:37,320 --> 00:05:43,100
+.. يعني بنسبة بقياس نسبة homoglobin A1C بإمكاني
+
+75
+00:05:43,100 --> 00:05:47,800
+اني انا اطلع على وضع السكر في الجسم خلال مثلا
+
+76
+00:05:47,800 --> 00:05:52,050
+التلات شهور اللي ماضين ك average timeتمام؟ إذا
+
+77
+00:05:52,050 --> 00:05:56,570
+نرتب أفكارنا شوية إحنا متفقين إنه ال glucose بيكون
+
+78
+00:05:56,570 --> 00:06:02,030
+موجود في ال blood يسري في ال blood ممكن إنه يفوت
+
+79
+00:06:02,030 --> 00:06:06,210
+على الخلايا تاخد الخلايا تصنع منه طاقة أحد الخلايا
+
+80
+00:06:06,210 --> 00:06:09,730
+اللي ممكن يدخل ال glucose لإلها هي ال RBCs حالها
+
+81
+00:06:09,730 --> 00:06:14,890
+حال أي خلية في الجسمولكن جزء من ال glucose هذا
+
+82
+00:06:14,890 --> 00:06:18,830
+بيروح بيرتبط مع ال homoglobin اللي موجود في ال ..
+
+83
+00:06:18,830 --> 00:06:23,250
+ال RBCs فبي .. بينتجلي حاجة اسمها glycated
+
+84
+00:06:23,250 --> 00:06:27,690
+homoglobin أو homoglobin A1C هلأ هذا ال homoglobin
+
+85
+00:06:27,690 --> 00:06:31,870
+A1C كل ما زاد السكر في الدم كل ما زاد دخول السكر
+
+86
+00:06:31,870 --> 00:06:35,970
+على الخلايا كل ما زاد دخوله على ال RBCs كل ما زاد
+
+87
+00:06:35,970 --> 00:06:39,650
+ارتباطه بال homoglobin إذا زاد .. هيزيد تركيزي ال
+
+88
+00:06:39,650 --> 00:06:45,840
+homoglobin A1Cتمام؟ و لإنه عمر خلية ال RBCs 120
+
+89
+00:06:45,840 --> 00:06:50,620
+يوم يعني هم 4 شهور بس أنا ك average ممكن أنا لو
+
+90
+00:06:50,620 --> 00:06:54,700
+جست ال homoglobin A1C في أي لحظة أكون يعني عندي
+
+91
+00:06:54,700 --> 00:07:00,240
+نظرة أو عندي تخمين كيف كان وضع السكر في الجسم خلال
+
+92
+00:07:00,240 --> 00:07:04,000
+التلت شهور الماضية خلينا نحكي مش الأربع شهور تمام؟
+
+93
+00:07:04,000 --> 00:07:10,620
+ك average period يعني كمدة هيك تقريبية تمام؟والله
+
+94
+00:07:10,620 --> 00:07:14,980
+إذا كان ال homoglobin A1C عالي تمام معناها إنه كان
+
+95
+00:07:14,980 --> 00:07:18,460
+في سكر كتير عالي في الجسم عشان هيك هو ارتبط مع ال
+
+96
+00:07:18,460 --> 00:07:21,120
+homoglobin بشكل كتير كبير و أعطانا تركيز ل
+
+97
+00:07:21,120 --> 00:07:25,080
+homoglobin A1C عالي هذه هي الفكرة إذا homoglobin
+
+98
+00:07:25,080 --> 00:07:29,820
+A1C this test provide an overview for your blood
+
+99
+00:07:29,820 --> 00:07:33,880
+sugar glucose level over a three month time span
+
+100
+00:07:34,440 --> 00:07:38,120
+خلال تلت شهور بيحكم ليه على وضرة السكر في الجسم،
+
+101
+00:07:38,120 --> 00:07:41,820
+هل كان فيه تنظيم، هل كان السكر طبيعي خلال الفترات
+
+102
+00:07:41,820 --> 00:07:47,840
+هذه، هل كان فيه ارتفاع او لا fasting blood glucose
+
+103
+00:07:47,840 --> 00:07:52,320
+fasting blood glucose اللي هو عبارة كان زمان نسميه
+
+104
+00:07:52,320 --> 00:07:55,620
+أو لساتنا يعني لازال الاسم دارش fasting blood
+
+105
+00:07:55,620 --> 00:08:00,140
+sugarتمام؟ سواء fasting blood glucose أو fasting
+
+106
+00:08:00,140 --> 00:08:03,960
+blood sugar هو نفس المعنى هو الفحص اللي احنا بنقيز
+
+107
+00:08:03,960 --> 00:08:09,840
+فيه مستوى السكر في الدم لشخص صايم تمام؟ على الأقل
+
+108
+00:08:09,840 --> 00:08:14,520
+تمان ساعات احنا زمان او في بداية المساق يعني مش
+
+109
+00:08:14,520 --> 00:08:18,480
+زمان كتير انه حكينا انه كل فحص له شروط وحكيتلكم
+
+110
+00:08:18,480 --> 00:08:22,120
+انه احيانا بيتطلب الفحص ان الشخص يكون صايم تمان
+
+111
+00:08:22,120 --> 00:08:28,940
+ساعات مثلاو لحتى ما تتأثر النتيجة فإذا فحص ال
+
+112
+00:08:28,940 --> 00:08:31,900
+fasting blood sugar أو fasting blood glucose هو
+
+113
+00:08:31,900 --> 00:08:38,140
+أحد الفحوصات اللي بتطلب انه المريض ييجي صايم على
+
+114
+00:08:38,140 --> 00:08:41,760
+الأقل تمان ساعات مش أقل منهم أو أكتر منهم يعني
+
+115
+00:08:41,760 --> 00:08:46,810
+بكتيرRandom blood glucose هو الفحص السكري
+
+116
+00:08:46,810 --> 00:08:50,930
+العشوائي، يعني أنه بدون تحضيرات، بدون ما الشخص
+
+117
+00:08:50,930 --> 00:08:54,730
+ييجي صايم، مش شرط إيش ما أكل، يعني فجأة هيك، يعني
+
+118
+00:08:54,730 --> 00:08:57,370
+في نص اليوم أو في نص النهار إجاك شخص يحكيلك و الله
+
+119
+00:08:57,370 --> 00:09:00,290
+بدي .. بدي أشوف وضع السكر عندي، طب صايم؟ لأ، ما
+
+120
+00:09:00,290 --> 00:09:04,530
+أكلت؟ أه، طب خلاص، ماشي، هذه طالما أنه أجى بدون
+
+121
+00:09:04,530 --> 00:09:09,960
+تحضيرات، بنسميها random blood glucoseإذن this test
+
+122
+00:09:09,960 --> 00:09:13,460
+is performed via blood draw at the lab when you
+
+123
+00:09:13,460 --> 00:09:17,200
+are not fasting إذا شخص مش صايم سحبناله عين الدم
+
+124
+00:09:17,200 --> 00:09:21,740
+بنجيسله random blood glucose معناها هادية الفحص
+
+125
+00:09:21,740 --> 00:09:26,040
+يعني بنسميه random blood glucose testit gives an
+
+126
+00:09:26,040 --> 00:09:29,440
+indication in two hours your body handle the
+
+127
+00:09:29,440 --> 00:09:34,560
+increased blood sugar level triggered by eating طب
+
+128
+00:09:34,560 --> 00:09:38,840
+إيش فايدة هذا الفحص؟ بيعطيني indication دلالة كيف
+
+129
+00:09:38,840 --> 00:09:43,280
+الجسم بيتعامل مع السكر أو نسبة الغذاء أو نسبة
+
+130
+00:09:43,280 --> 00:09:47,240
+السكريات اللي أنا تناولتها أو ال carbohydrate اللي
+
+131
+00:09:47,240 --> 00:09:54,560
+تناولتها على الأقل قبل ساعتين هلتخلص منها أو نظمها
+
+132
+00:09:54,560 --> 00:10:00,560
+أو لا، تمام؟ هذه الفائدة الفحص هذه طبعا a random
+
+133
+00:10:00,560 --> 00:10:04,080
+blood glucose test result over 200 mg per
+
+134
+00:10:04,080 --> 00:10:07,440
+deciliter may indicate diabetes إذا كانت العيلة
+
+135
+00:10:07,440 --> 00:10:12,010
+العشوائيةنتيجة السكر فيها أعلى من 200 milligram
+
+136
+00:10:12,010 --> 00:10:16,310
+per deciliter معناها هذا الشخص بنشك أن هو عنده
+
+137
+00:10:16,310 --> 00:10:19,710
+diabetes ممكن يكون دلالة على ال diabetes فانا بروح
+
+138
+00:10:19,710 --> 00:10:24,070
+بتأكد بفحوصات تانية جابل أحكم أن هذا الشخص مريض
+
+139
+00:10:24,070 --> 00:10:29,110
+diabetes، تمام؟لازم انه على الأقل .. على الأقل
+
+140
+00:10:29,110 --> 00:10:36,010
+خلال ساعتين يكون ال .. السكر في الدم رجع للطبيعي
+
+141
+00:10:36,010 --> 00:10:41,110
+أو أقل من 140 milligram per deciliter، تمام؟ فإذا
+
+142
+00:10:41,110 --> 00:10:47,530
+كان يعني جاني شخص ..يعني بده اعمله random blood
+
+143
+00:10:47,530 --> 00:10:51,850
+glucose test عينة عشوائية بدون fasting فبسأله مثلا
+
+144
+00:10:51,850 --> 00:10:56,690
+اه امتى اخر مرة تناول طعام حكالي خلال ساعة ساعتين
+
+145
+00:10:56,690 --> 00:11:00,950
+مثلا ماشي okay تمام ب .. بجريله الفحص ولكن بكون
+
+146
+00:11:00,950 --> 00:11:04,740
+حاطة في الاعتبار ان هو والله ماكل ..جابل الساعتين
+
+147
+00:11:04,740 --> 00:11:09,880
+ما ي .. ما يخلصوا يعني انه .. انه أخر مرة أكل فيها
+
+148
+00:11:09,880 --> 00:11:14,580
+كان المدة ما بينها و ما بين أخد العينة أقل من
+
+149
+00:11:14,580 --> 00:11:19,640
+ساعتين إذا ساعتين تمام لازم النتيجة ما تكونش ..
+
+150
+00:11:19,640 --> 00:11:23,020
+يعني ما تكونش تتخطى ال 200 milligram per deciliter
+
+151
+00:11:23,020 --> 00:11:27,360
+لإن هاي معناها indicate diabetes glucose tolerance
+
+152
+00:11:27,360 --> 00:11:32,880
+testاللي هو الفحص اللي أنا بيجيني الشخص صايم أول
+
+153
+00:11:32,880 --> 00:11:37,760
+إشي بعمله fasting blood glucose لما بيجي صايم و
+
+154
+00:11:37,760 --> 00:11:42,900
+بعد هيك أنا بعطيه محلول سكري فيه خمسة و سبعين جرام
+
+155
+00:11:42,900 --> 00:11:49,790
+من ال glucoseو ببلش أقيسله السكر تمام بيصير هيك
+
+156
+00:11:49,790 --> 00:11:53,110
+random صار لما أنا بدى أعمله افحاصات سكر بعد ما
+
+157
+00:11:53,110 --> 00:11:57,330
+أكل خمسة و سبعين جرام صارت random تمام؟ فبعمله فحص
+
+158
+00:11:57,330 --> 00:12:03,630
+خلال مثلا بعد نص ساعة، بعد ساعة، بعد ساعتينتمام؟ و
+
+159
+00:12:03,630 --> 00:12:06,130
+في أحيانا لأ بيعملوا بس على ال fasting و بعد
+
+160
+00:12:06,130 --> 00:12:09,870
+ساعتين، في لأ بيعملوا على ال fasting لما بيجيني
+
+161
+00:12:09,870 --> 00:12:14,750
+صايم و في بعد نص ساعة من تناوله ال glucose drink و
+
+162
+00:12:14,750 --> 00:12:18,900
+بعد ساعة .. بعد ساعة و نص .. بعد ساعتينتمام؟ إذا
+
+163
+00:12:18,900 --> 00:12:22,580
+هو هذا عبارة عن multi-test لـ Glucose Tolerance
+
+164
+00:12:22,580 --> 00:12:28,580
+Test هو يعني أكتر من فحص في واحد أول إشي بعمله عال
+
+165
+00:12:28,580 --> 00:12:34,140
+fasting تمام؟ بعدين بعمله بعد ما ياكل 75 gram كذا
+
+166
+00:12:34,140 --> 00:12:37,620
+random يعني إما random واحد بعد ساعتين أو كل
+
+167
+00:12:37,620 --> 00:12:42,680
+مصارعة بعمله random testتمام؟ ويبرسم curve في
+
+168
+00:12:42,680 --> 00:12:47,040
+القيم اللي طلعت معايا لازم تكون حكينا النتيجة خلال
+
+169
+00:12:47,040 --> 00:12:51,660
+الساعتين هدول انه رجعت لأ الطبيعي او على الأقل
+
+170
+00:12:51,660 --> 00:12:57,790
+تكون اقل من 140 milligram per deciliterطبعا إذا
+
+171
+00:12:57,790 --> 00:13:03,650
+أنا الشخص أجاني عملتله random و طلع القيمة تبعته
+
+172
+00:13:03,650 --> 00:13:07,290
+أكتر من 200 فعلى طول باتجه لل blood .. لل fasting
+
+173
+00:13:07,290 --> 00:13:11,010
+blood glucose لجيته عالي على طول باتجه لل glucose
+
+174
+00:13:11,010 --> 00:13:15,170
+tolerance test لجيت أيضا هو abnormal فخلاص يعني
+
+175
+00:13:15,170 --> 00:13:19,030
+مابكون شبه متأكدة أن هو الشخص هذا يعاني من
+
+176
+00:13:19,030 --> 00:13:24,370
+diabetes مرض السكريطيب إيش مبدأ أعمل الفحص؟ the
+
+177
+00:13:24,370 --> 00:13:28,530
+principle of glucose test هلأ ال glucose مو .. وين
+
+178
+00:13:28,530 --> 00:13:31,330
+موجود هيكون؟ موجود في البلازمة، إذا العيلة تبع ..
+
+179
+00:13:31,330 --> 00:13:35,890
+العينة تبعتى بدها تكون إما بلازمة أو حتى سيرم، ما
+
+180
+00:13:35,890 --> 00:13:38,710
+هو السيرم هو نفسه البلازمة، بس اللهم إنه فقد ال
+
+181
+00:13:38,710 --> 00:13:43,390
+fibrinogenتمام، إذا العينة تبعتي هتكون إما plasma
+
+182
+00:13:43,390 --> 00:13:47,610
+أو serum، إذا ال blood اللي أنا بدي أجمعه من
+
+183
+00:13:47,610 --> 00:13:52,770
+المريض بدي أفصله على جهاز ال centrifuge علشان أحصل
+
+184
+00:13:52,770 --> 00:13:57,600
+إما على plasma أو serumهذا ال .. ال .. طبعا رح
+
+185
+00:13:57,600 --> 00:14:03,060
+أضيف كمية من ال plasma على ال .. ال reagent تبعي
+
+186
+00:14:03,060 --> 00:14:06,660
+طبعا احنا حكينا .. عرفنا المحليل قبل هيك و حكينا
+
+187
+00:14:06,660 --> 00:14:10,780
+إنه لازم يكون في عندي reagent هو اللي بيجري تغيير
+
+188
+00:14:10,780 --> 00:14:14,160
+ما، change معين على العينة، ممكن هذا التغيير يكون
+
+189
+00:14:14,160 --> 00:14:18,320
+measurable، يمكن قياسه، و هنا الفكرة اليوم ان شاء
+
+190
+00:14:18,320 --> 00:14:21,260
+الله رح نوظف إيش معنا كلمة reagent و إيش معنا كلمة
+
+191
+00:14:21,260 --> 00:14:27,810
+standardتمام؟ فال reagent تبعي بيكون موجود فيه
+
+192
+00:14:27,810 --> 00:14:31,070
+انزيم .. هاي طبعا .. هاي ال glucose جاي من وين؟
+
+193
+00:14:31,070 --> 00:14:34,750
+جاي من العينة تبعتي، من ال plasma، مع oxygen، مع
+
+194
+00:14:34,750 --> 00:14:40,010
+water، جاي أيضا من العينة تبعتي ال reagent glucose
+
+195
+00:14:40,010 --> 00:14:44,730
+.. موجود فيه اللي هو ال glucose oxidase enzyme هي
+
+196
+00:14:44,730 --> 00:14:50,370
+oxid ال glucose ويحوله من glucose لgluconate أو
+
+197
+00:14:50,370 --> 00:14:55,870
+لgluconic acidمع hydrogen peroxide، هذه الـ
+
+198
+00:14:55,870 --> 00:15:00,650
+hydrogen peroxide رح تتفاعل مع مكون أخر من .. أو
+
+199
+00:15:00,650 --> 00:15:04,150
+مكونات أخرى من الـ reagent اللي هي الأربع
+
+200
+00:15:04,150 --> 00:15:07,810
+أمينوفينازون مع فينول في وجود أنزايم اللي هو
+
+201
+00:15:07,810 --> 00:15:12,690
+peroxideaseهذا طبعا ال amino phenazone هو عبارة عن
+
+202
+00:15:12,690 --> 00:15:16,650
+oxygen receptor هياخد oxygen من ال peroxide و يتحد
+
+203
+00:15:16,650 --> 00:15:20,230
+مع الفينول و يعطيهم quinamine و هذا ال quinamine
+
+204
+00:15:20,230 --> 00:15:23,710
+اللي هو عبارة عن colored complex لونه pink لونه
+
+205
+00:15:23,710 --> 00:15:28,710
+زهري تمام؟فإنا .. إذا أنا بقيس التغير في اللون
+
+206
+00:15:28,710 --> 00:15:32,330
+العينة طبيعتي بتكون اللون ال straw color لما أضيف
+
+207
+00:15:32,330 --> 00:15:36,910
+عليها ال reagent و أعطيها مدة للتفاعل راح تحقي ..
+
+208
+00:15:36,910 --> 00:15:39,990
+تعطيني اللون ال pink هذا دليل على إنه ال reagent
+
+209
+00:15:39,990 --> 00:15:44,630
+عمل exchange على العينة تبعتي و هذا ال exchange
+
+210
+00:15:44,630 --> 00:15:48,330
+اللي هي تغير في اللون أنا بدي أقيصه باستخدام جهاز
+
+211
+00:15:48,330 --> 00:15:51,950
+معيناللي هو جهاز ال spectrophotometer اللي احنا
+
+212
+00:15:51,950 --> 00:15:57,770
+راح نتحدث عنه تركيبه أجزاءه وظيفته فكرة عمله كلها
+
+213
+00:15:57,770 --> 00:16:04,270
+راح نحكي عنها الإسبوع اللي جاي طيب فإذا الفكرة
+
+214
+00:16:04,270 --> 00:16:07,130
+خلينا نشوف إيش مكتوب لجلوكوز اللي موجود في
+
+215
+00:16:07,130 --> 00:16:11,630
+البلازمة راح يتأكسد بفعل ال enzyme لجلوكوز
+
+216
+00:16:11,630 --> 00:16:15,690
+اكسيدازي اللي موجود في ال reagent و هيحوله إلى
+
+217
+00:16:15,690 --> 00:16:20,790
+gluconic acid أو gluconateطبعا و مع تحرر hydrogen
+
+218
+00:16:20,790 --> 00:16:27,010
+peroxide hydrogen peroxide اللي هي هتتحول لماء
+
+219
+00:16:27,010 --> 00:16:32,150
+بفعل ال peroxidase و طبعا الإلكترونات أو ال oxygen
+
+220
+00:16:32,150 --> 00:16:35,560
+اللي راح يتحرر منهاراح يرتبط مع الأربع
+
+221
+00:16:35,560 --> 00:16:39,900
+أمينوفينازون لإنها oxygen receptor و هذا ال oxygen
+
+222
+00:16:39,900 --> 00:16:44,040
+receptor راح أيضا يتحد مع ال phenol ويعطيني اللي
+
+223
+00:16:44,040 --> 00:16:47,480
+هو ال colored chromogene المادة الملوقة المنتجة
+
+224
+00:16:47,480 --> 00:16:51,120
+لللون chromogene يعني منتجة لللون chromolone و
+
+225
+00:16:51,120 --> 00:16:55,520
+gene منتج producingفإذا color reproducing و
+
+226
+00:16:55,520 --> 00:16:58,440
+هيعطيني لون اللي هو اللون ال pink اللي أنا بقيسه
+
+227
+00:16:58,440 --> 00:17:01,540
+باستخدام جهاز المقياف الدوقي أو ال
+
+228
+00:17:01,540 --> 00:17:05,660
+spectrophotometer اللي هو على طول موجي 500
+
+229
+00:17:05,660 --> 00:17:10,320
+nanometer حكينا إنه هذا الجهاز راح نتحدث عنه
+
+230
+00:17:10,320 --> 00:17:15,800
+الإسبوع الجايلـ clinical significance إيش أهمية أو
+
+231
+00:17:15,800 --> 00:17:19,640
+الأهمية الطبية لفحص ال glucose test the detection
+
+232
+00:17:19,640 --> 00:17:24,080
+of glucose in body fluid is important in the
+
+233
+00:17:24,080 --> 00:17:29,320
+diagnosis of diabetes إذا هو مهم جدا في تشخيص مرض
+
+234
+00:17:29,320 --> 00:17:33,440
+السكر تمام اللي هو يعني ارتفاع السكر في الدم،
+
+235
+00:17:33,440 --> 00:17:37,380
+diabetes يعني ارتفاع السكر في الدم و أيضا للكشف عن
+
+236
+00:17:37,380 --> 00:17:41,500
+حالات الhypoglycemiaطيب خلينا بس نشوف إيش يعني
+
+237
+00:17:41,500 --> 00:17:46,160
+كلمة hypoglycemia hypo يعني low، glyce يعني
+
+238
+00:17:46,160 --> 00:17:49,820
+glucose وحكيتلكم emia يعني في الدم، وين ما تشوفي
+
+239
+00:17:49,820 --> 00:17:54,400
+كلمة emia يعني في الدم، إذا ال .. ال hypo low
+
+240
+00:17:54,400 --> 00:17:58,660
+blood glucose level in .. يعني أو low blood
+
+241
+00:17:58,660 --> 00:18:03,170
+glucose in bloodلو blood glucose level، هيك هيك
+
+242
+00:18:03,170 --> 00:18:09,110
+بتصير، تمام؟ هيبقوا لو glycemia glucose level in
+
+243
+00:18:09,110 --> 00:18:14,920
+blood، low level of glucose in bloodطبعا؟ و طيب لو
+
+244
+00:18:14,920 --> 00:18:20,200
+أنا بدي أعبر عن ال diabetes بهذا ال .. ب .. ب term
+
+245
+00:18:20,200 --> 00:18:23,740
+أو بمصطلح مشابه لهذا إيش بدي أحكي؟ ال diabetes
+
+246
+00:18:23,740 --> 00:18:28,320
+إحنا حكينا ارتفاع السكر في الدم فإذا بدها تصير
+
+247
+00:18:28,320 --> 00:18:34,080
+hyperglycemia إذا hyperglycemia اللي هي يعني
+
+248
+00:18:34,080 --> 00:18:37,980
+diabetes طبعا؟ أو ارتفاع مستوى السكر في الدم
+
+249
+00:18:39,300 --> 00:18:43,120
+hyperiglycemia hyperارتفاع إجليسيميا حكينا السكر
+
+250
+00:18:43,120 --> 00:18:47,280
+في الدم طب العين اللي أنا بستخدمها حكينا إما تكون
+
+251
+00:18:47,280 --> 00:18:54,760
+serum أو plasma ال plasma هي الخيار الأمثل لقياس
+
+252
+00:18:54,760 --> 00:19:00,070
+مستوى السكر في الدمو طبعا ال plasma glucose level،
+
+253
+00:19:00,070 --> 00:19:04,890
+نسبة السكر في ال plasma بتكون ثابتة خلال 6 ساعات
+
+254
+00:19:04,890 --> 00:19:08,210
+إذا ضلت العينة في ال room temperature، يعني أنا
+
+255
+00:19:08,210 --> 00:19:12,130
+حاطة ال plasma في ال room temperature، بيضل نسبة
+
+256
+00:19:12,130 --> 00:19:17,210
+السكر ثابتة لمدة 6 ساعات، ممكن تضل ثابتة ل 24 ساعة
+
+257
+00:19:17,210 --> 00:19:22,370
+إذا أنا حاطتها في التلاجةIt's important that the
+
+258
+00:19:22,370 --> 00:19:26,150
+plasma should be separated from the cell soon
+
+259
+00:19:26,150 --> 00:19:31,230
+after collection طبعا هذا احنا بنحكي عن ثابت مستوى
+
+260
+00:19:31,230 --> 00:19:35,090
+الجلوكوز في البلازمة طبعا لازم تكون البلازمة
+
+261
+00:19:35,090 --> 00:19:39,850
+منفصلة عن الخلايا لإنه احنا اذا مافصلناها عن
+
+262
+00:19:39,850 --> 00:19:44,570
+الخلايا فالخلايا راح تظل تسحب جلوكوز يعني هي خلايا
+
+263
+00:19:44,570 --> 00:19:49,090
+عايشة وعم تعمل glycolysis و بتنتج طاقةو بتشوف
+
+264
+00:19:49,090 --> 00:19:52,150
+شغلها فممكن تسحب ال glucose اللي موجود في ال
+
+265
+00:19:52,150 --> 00:19:55,870
+plasma فيبين عندي مثلا انه ال glucose ممكن يكون
+
+266
+00:19:55,870 --> 00:19:59,750
+طبيعي او اقل من الطبيعي و المشكلة تكون عندي سببها
+
+267
+00:19:59,750 --> 00:20:04,130
+ان انا مافصلتش ال plasma عن الخلايا اذا لازم افصل
+
+268
+00:20:04,130 --> 00:20:08,030
+ال plasma عن الخلايا على الأقل خلال نص ساعة من سحب
+
+269
+00:20:08,030 --> 00:20:13,530
+العينة و يفضل كمان اقل من نص ساعةلحتى الخلايا
+
+270
+00:20:13,530 --> 00:20:19,050
+حكينا ما تستهلك السكر اللي موجود في البلازمة، أنا
+
+271
+00:20:19,050 --> 00:20:23,690
+بدي أقيس مستوى السكر اللي في البلازمة عشان أاخد
+
+272
+00:20:23,690 --> 00:20:26,850
+indication دلالة على وضع المريض، هو عنده
+
+273
+00:20:26,850 --> 00:20:31,610
+hyperglycemia ولا عنده hypoglycemiaفما بدي عامل
+
+274
+00:20:31,610 --> 00:20:34,970
+آخر ييجي يأثر على النتيجة زي مثلا وجود الخلايا
+
+275
+00:20:34,970 --> 00:20:39,610
+هتستهلك السكر فهتأثرلي على النتيجة انا مش هقيز كل
+
+276
+00:20:39,610 --> 00:20:44,110
+مستوى السكر ساعتها لإن في جزء تماشي استهلاكه
+
+277
+00:20:44,110 --> 00:20:48,950
+بواسطة الخلايا فلأ يفضل فصل الملازمة عن الخلايا
+
+278
+00:20:48,950 --> 00:20:55,740
+على طول فورا بعد سحب العينة أفضلأو خلال نص ساعة
+
+279
+00:20:55,740 --> 00:20:58,900
+طيب التجربة تبعتي إيش بدني أعمل؟ بدي أجيب تلت
+
+280
+00:20:58,900 --> 00:21:04,560
+قنابيد و أعلمهم انبوبة بسميها blank انبوبة بسميها
+
+281
+00:21:04,560 --> 00:21:10,980
+standard و انبوبة بسميها ال sample أو ال testطيب
+
+282
+00:21:10,980 --> 00:21:14,020
+الانبوبة الأولى blank لا يوجد فيها غير ال reagent
+
+283
+00:21:14,020 --> 00:21:17,040
+هي فاضية مافيش فيها أي مادة تانية يتفاعل معها ال
+
+284
+00:21:17,040 --> 00:21:23,580
+reagent لا standard ولا sample طيب التقيوب اللي
+
+285
+00:21:23,580 --> 00:21:27,520
+فيها standard فيها ال reagent مع مين؟ مع ال
+
+286
+00:21:27,520 --> 00:21:32,540
+standardوالتيوب اللي فيها ال sample هيكون فيها ال
+
+287
+00:21:32,540 --> 00:21:36,940
+reagent مع ال sample، اذا التيوب ال standard هيكون
+
+288
+00:21:36,940 --> 00:21:40,160
+فيها reagent و standard وتيوب ال sample هيكون فيها
+
+289
+00:21:40,160 --> 00:21:45,270
+ال reagent و ال sample أو ال specimenطيب احنا
+
+290
+00:21:45,270 --> 00:21:49,730
+بتتذكروا المحاضرة اليدوية اللي كتبتها بإيدي و لما
+
+291
+00:21:49,730 --> 00:21:54,090
+رسمتلكم two tubes و حكيتلكم هي في عينة فيها أو
+
+292
+00:21:54,090 --> 00:21:57,150
+tube فيها عينة أو tube فيها standard و بضيف عليهم
+
+293
+00:21:57,150 --> 00:22:01,210
+reagent هيصير فيهم تغير في اللون و طبعا ال
+
+294
+00:22:01,210 --> 00:22:05,410
+standard بيكون معلوم التركيز فانا بجيب ال .. يعني
+
+295
+00:22:05,410 --> 00:22:09,050
+اذا كانت تغير باللون مثلافي عينة ال sample ضعف
+
+296
+00:22:09,050 --> 00:22:11,550
+التغير في ال standard معناها انه التركيز في ال
+
+297
+00:22:11,550 --> 00:22:15,730
+sample ضعف التغير التركيز في ال standard وطالما
+
+298
+00:22:15,730 --> 00:22:18,870
+واذا كان ال standard تركيزه مثلا مية معناها العينة
+
+299
+00:22:18,870 --> 00:22:22,770
+هيكون تركيزها 200 اذا انا بجيب تركيز ال sample
+
+300
+00:22:22,770 --> 00:22:27,070
+المجهول بدلالة المعلوم اللي هو ال standard اذا ان
+
+301
+00:22:27,070 --> 00:22:30,630
+هي ان هي عرفت ال sample ايش وظيفتها هالتيوب والله
+
+302
+00:22:30,630 --> 00:22:34,450
+انا بقيس فحص او بعمل فحص لمريض طبيعي يعني بدي ا ..
+
+303
+00:22:34,450 --> 00:22:38,460
+بدي يكون عندي تيوب فيها العينة تبعتهطيب إيش أهمية
+
+304
+00:22:38,460 --> 00:22:42,000
+ال standard؟ والله هذا محلول معلوم التركيز من
+
+305
+00:22:42,000 --> 00:22:45,640
+السكر، يحتوي على سكر فقط، سكر و ماء، ولكن معلوم
+
+306
+00:22:45,640 --> 00:22:53,660
+التركيز، تمام؟ و هذا المحلول، المعلوم التركيز أنا
+
+307
+00:22:53,660 --> 00:22:58,140
+بدلالته بجيب المجهول اللي موجود في ال sample طيب
+
+308
+00:22:58,140 --> 00:23:02,580
+إيش وظيفة ال blank؟مش وظيفة ال blank اللي هو
+
+309
+00:23:02,580 --> 00:23:05,980
+الفاضي هذا ال blank يعني فاضي مش فيه إشي غير agent
+
+310
+00:23:05,980 --> 00:23:10,780
+طيب ال وظيفة tube ل blank إحنا حكينا إنه النتيجة
+
+311
+00:23:10,780 --> 00:23:15,040
+أنا بقرأها .. بقرأ شدة التغير في اللون أو ال ..
+
+312
+00:23:15,040 --> 00:23:18,400
+بقرأ اللون تمام باستخدام جهاز ال spectrophotometer
+
+313
+00:23:19,140 --> 00:23:22,940
+طيب ال .. طب أنا ب .. بقيس التغير في اللون بال
+
+314
+00:23:22,940 --> 00:23:26,540
+standard و التغير في ال .. ال .. اللون في ال
+
+315
+00:23:26,540 --> 00:23:31,120
+sample و بقارنهم مع بعض طب إيش وظيفة ال blank؟ طيب
+
+316
+00:23:31,120 --> 00:23:36,180
+هو مش ال reagent نفسه إله لون؟ أه إله لون طيب أنا
+
+317
+00:23:36,180 --> 00:23:41,170
+لازم ألغي اللون اللي جاي من ال reagentتمام؟ علشان
+
+318
+00:23:41,170 --> 00:23:45,230
+بس لما أقيس على الجهاز أقيس اللون اللي جاي فقط من
+
+319
+00:23:45,230 --> 00:23:48,590
+تفاعل ال reagent مع ال standard أو من تفاعل ال
+
+320
+00:23:48,590 --> 00:23:53,910
+reagent مع ال sample في tube ال sample تمام؟ فإذا
+
+321
+00:23:53,910 --> 00:23:58,930
+ال .. ال blank بس علشان أصفر عليه و ألغي اللون
+
+322
+00:23:58,930 --> 00:24:04,930
+اللي جاي من ال reagent تمام؟إذا بحضر التلات قنابيب
+
+323
+00:24:04,930 --> 00:24:08,730
+بحط في كل انبوبة one ML من ال reagent بعد ما
+
+324
+00:24:08,730 --> 00:24:12,610
+أعلمهم بعد ما أعمللهم labeling و ال blank بسيبها
+
+325
+00:24:12,610 --> 00:24:16,450
+فاضية ال standard بضيف عليها عشرة microliter من ال
+
+326
+00:24:16,450 --> 00:24:19,530
+standard و عينة ال sample بضيف عليها عشرة
+
+327
+00:24:19,530 --> 00:24:24,490
+microliter من عينة المريض اللي هي إما تكون plasma
+
+328
+00:24:24,490 --> 00:24:29,490
+أو serum و بعد هيك بعمل mixing منيح للقنابيب و
+
+329
+00:24:29,490 --> 00:24:32,590
+بعمل incubation يعني بتركها تتفاعل فترة التفاعل
+
+330
+00:24:32,590 --> 00:24:37,040
+incubationحضانة فترة التفاعل لمدة عشر دقائق على
+
+331
+00:24:37,040 --> 00:24:40,420
+درجة حرارة الغرفة أو خمس دقائق في ال incubator على
+
+332
+00:24:40,420 --> 00:24:44,320
+درجة حرارة سبعة وتلاتين أو في ال water bath في ال
+
+333
+00:24:44,320 --> 00:24:49,840
+water bath تمام measure the absorbance of the
+
+334
+00:24:49,840 --> 00:24:54,260
+standard بقيس التغير في النون من خلال ال ..
+
+335
+00:24:54,260 --> 00:24:59,580
+باستخدام الجهاز و طبعا against the blank لازم أصفر
+
+336
+00:24:59,580 --> 00:25:02,720
+على ال blankبقيس التغير في اللون في ال standard و
+
+337
+00:25:02,720 --> 00:25:06,900
+في ال specimen tube على طول موجي 500 nanometer و
+
+338
+00:25:06,900 --> 00:25:09,800
+القراءة ممكن تظل ثابتة يعني اللي أنا بيبهد لما
+
+339
+00:25:09,800 --> 00:25:13,320
+أحضرها لو انشغلت عنها ممكن خلال ساعة أرجعلها و
+
+340
+00:25:13,320 --> 00:25:16,720
+أشتغللها أكثر من ساعة لأ بيكون صار تغير في النتيجة
+
+341
+00:25:16,720 --> 00:25:22,080
+يعني ممكنش النتيجة دقيقةطيب بعد هيك أنا جيبت
+
+342
+00:25:22,080 --> 00:25:25,100
+القراقة أو التغير اللى فى اللون اللى حدث فى ال
+
+343
+00:25:25,100 --> 00:25:28,140
+sample على التغير اللى فى اللون اللى حدث فى ال
+
+344
+00:25:28,140 --> 00:25:32,640
+standard ضرب تركيز ال standard بجيب هيك تركيز ال
+
+345
+00:25:32,640 --> 00:25:36,540
+glucose ووحدة القياس milligram per deciliter هذا
+
+346
+00:25:36,540 --> 00:25:42,560
+القانون بنسميه peers lambert law و قانون peers و
+
+347
+00:25:42,560 --> 00:25:46,840
+lambert ورح نشرحه ان شاء الله لما نحكى على جهاز ال
+
+348
+00:25:46,840 --> 00:25:49,840
+spectrophotometer نعرف من وين أجى هذا القانون
+
+349
+00:25:50,170 --> 00:25:55,050
+linearity إيش يعني ال linearity؟ يعني أقصى قيمة بي
+
+350
+00:25:55,050 --> 00:26:00,370
+.. بيقيصها ال .. ال .. الطريقة هذه أو ال .. يعني
+
+351
+00:26:00,370 --> 00:26:05,310
+الفحص طبيعي هنا في الطريقة هاي اللي ذكرناها طبعا،
+
+352
+00:26:05,310 --> 00:26:10,830
+طريقة ل .. لglucose oxidase و ال .. ال peroxidase
+
+353
+00:26:10,830 --> 00:26:17,110
+في هاي الطريقة و إنتاج الكلو .. الكوينونأعلى قيمة
+
+354
+00:26:17,110 --> 00:26:19,870
+بيقيسها بتقيسها هاي الطريقة 500 milligram per
+
+355
+00:26:19,870 --> 00:26:23,070
+deciliter يعني لو العينة تركيزها أعلى من 500
+
+356
+00:26:23,070 --> 00:26:26,370
+milligram per deciliter الطريقة هاي مش هتتمكن من
+
+357
+00:26:26,370 --> 00:26:30,230
+قياسها و لازم انا إيش أعمل؟ أعمل dilution لل
+
+358
+00:26:30,230 --> 00:26:35,570
+sample أخفف واحد لاتنين مثلا و بعد هيك لما بجيب
+
+359
+00:26:35,570 --> 00:26:38,990
+القراءة بدربها باتنين اللي هو ال dilution factor
+
+360
+00:26:38,990 --> 00:26:43,850
+علشان أحصل على التركيز الأصلي زي ما احنا اتعلمنا
+
+361
+00:26:43,850 --> 00:26:49,620
+في محاضرة ال dilutionطيب إيش يعني .. طيب من وين
+
+362
+00:26:49,620 --> 00:26:53,080
+جاية كلمة linearity؟ شوفوا معايا هو العلاقة ما بين
+
+363
+00:26:53,080 --> 00:26:55,920
+ال absorbance اللي هي القراءة اللي بيعطينا إياها
+
+364
+00:26:55,920 --> 00:27:00,540
+الجهاز و التركيز دايما علاقة تردية يعني أو خلينا
+
+365
+00:27:00,540 --> 00:27:03,340
+نحكي غالبا بتكون علاقة تردية في بعض الفحوصات
+
+366
+00:27:03,340 --> 00:27:07,180
+بتكونش علاقة تردية بس خلينا نحكي غالبا الغالب أنه
+
+367
+00:27:07,180 --> 00:27:11,020
+كل ما زاد التركيز هتزيد التفاعل فهيزيد شدة اللون
+
+368
+00:27:11,020 --> 00:27:14,940
+اللي ناتج عن التفاعل فهتزيد قراءة ال .. ال ..
+
+369
+00:27:14,940 --> 00:27:19,090
+الجهازفإذا القراءة اللي بيعطيني الجهة جهاز تتناسب
+
+370
+00:27:19,090 --> 00:27:24,130
+ترضيا مع التركيز في العينة طبعا طيب لو زادت .. لو
+
+371
+00:27:24,130 --> 00:27:27,210
+زادت التركيز في العينة كتير كتير كتير يعني تجاوز
+
+372
+00:27:27,210 --> 00:27:31,010
+مثلا ال 500 milligram per deciliter طب ما هو ال ..
+
+373
+00:27:31,010 --> 00:27:35,110
+يعني أنا ماعنديش كمية reaction كفاية تتفاعل مع كل
+
+374
+00:27:35,110 --> 00:27:37,950
+هال glucose اللي موجود في العينة عشان أول إشي
+
+375
+00:27:37,950 --> 00:27:43,190
+يعطيني شدة لون كافيةتانيا لو أعطاني شدة لون عالية
+
+376
+00:27:43,190 --> 00:27:46,830
+الجهاز نفسه ممكن مايتحمل يقرأها يعني مايستطيع ..
+
+377
+00:27:46,830 --> 00:27:51,370
+يعني مايستطيع يقرأها فبالتالي تنعدم العلاقة
+
+378
+00:27:51,370 --> 00:27:55,130
+الترضية ما بين ال .. ال .. ال absorbance أو القيمة
+
+379
+00:27:55,130 --> 00:28:00,620
+اللي بيعطينيها الجهاز و التركيزفبالتالي احنا بنحكي
+
+380
+00:28:00,620 --> 00:28:04,660
+والله هذا الفحص بتضل العلاقة ترضية ما بين القراءة
+
+381
+00:28:04,660 --> 00:28:08,560
+اللي على الجهاز و التركيز لحد قيمة خمسمية زادت
+
+382
+00:28:08,560 --> 00:28:12,280
+القي التركيز في العين عن خمسمية بيبدأ الجهاز يخربط
+
+383
+00:28:12,280 --> 00:28:17,220
+بيبدأ يعطيني قراءة .. قراءات او شدة لون مالهاش
+
+384
+00:28:17,220 --> 00:28:21,480
+علاقة بالتركيز مش ترضية مية لمية العلاقة فبتنعدم
+
+385
+00:28:21,480 --> 00:28:25,800
+ال linearity بتنعدم العلاقة الخطية ما بينهم تمام؟
+
+386
+00:28:25,800 --> 00:28:31,330
+فهي هي الفكرةال reference value القيمة الطبيعية ل
+
+387
+00:28:31,330 --> 00:28:35,890
+fasting blood glucose أو ال plasma glucose إذا
+
+388
+00:28:35,890 --> 00:28:39,610
+الشخص صايم طبعا من 70 ل110 ميلي جرام per
+
+389
+00:28:39,610 --> 00:28:44,030
+deciliter، هذا الطبيعي ال postprandial plasma
+
+390
+00:28:44,030 --> 00:28:47,890
+glucose ال two hour يعني بعد وجبات أو بين الوجبات
+
+391
+00:28:48,030 --> 00:28:53,410
+ولكن يعني بعد .. بعد ساعتين مثلا من الواجبة لازم
+
+392
+00:28:53,410 --> 00:28:56,950
+يكون اقل من 140 ميلي جرام per deciliter و هذه
+
+393
+00:28:56,950 --> 00:29:01,430
+المعلومة احنا حاكيناها انه سواء في ال random او في
+
+394
+00:29:01,430 --> 00:29:08,780
+ال glucose oral tolerance test سواء كانيعني الشخص
+
+395
+00:29:08,780 --> 00:29:14,280
+جيتله .. عملتله عينة random أو بال .. بال GOT اللي
+
+396
+00:29:14,280 --> 00:29:19,320
+هو ال glucose tolerance test لازم يعني الشخص خلال
+
+397
+00:29:19,320 --> 00:29:24,120
+ساعتين نسبة السكر تكون رجعت للطبيعي أو أتكون أقل
+
+398
+00:29:24,120 --> 00:29:28,950
+من 140 milligram per deciliterو في ال random في
+
+399
+00:29:28,950 --> 00:29:31,910
+العينة ال random برضه كمان حكينا لازم تكون اقل من
+
+400
+00:29:31,910 --> 00:29:34,950
+140 milligram per deciliter اذا كانت اكتر من 200
+
+401
+00:29:34,950 --> 00:29:38,750
+ببدأ شك ان الشخص هذا عنده diabetes فلازم اروح
+
+402
+00:29:38,750 --> 00:29:43,490
+اتأكد بفحوصات تانية serum glucose value are 1
+
+403
+00:29:43,490 --> 00:29:49,150
+point 15% lower than plasma glucose value يعني
+
+404
+00:29:49,150 --> 00:29:57,760
+دايما لو اجاني شخصيعني صايم و أنا عملتله فحص سكر
+
+405
+00:29:57,760 --> 00:30:02,020
+fasting blood sugar على عينة أخدت سحب منه عينة و
+
+406
+00:30:02,020 --> 00:30:05,820
+فضيتها واحدة في إيديتا تيوب و واحدة في بلان تيوب
+
+407
+00:30:05,820 --> 00:30:09,840
+الإيديتا هتعطيني بلازمة و البلان تيوب هتعطيني
+
+408
+00:30:09,840 --> 00:30:14,710
+سيرامتمام؟ لو انا خدت .. طبعا انا الشخص نفسه في
+
+409
+00:30:14,710 --> 00:30:18,750
+نفس اللحظة سحبت العينا و هو جاييني صايم بس اللهم
+
+410
+00:30:18,750 --> 00:30:22,450
+ان واحدة عمل .. خليتها تعطيني plasma يعني جزء
+
+411
+00:30:22,450 --> 00:30:26,030
+فصلته plasma من العينا و جزء فصلته يعطيني serum
+
+412
+00:30:26,840 --> 00:30:30,600
+تمام؟ و لو مشيت الفحص مع بعض و روحت اشتغلتلهم للـ
+
+413
+00:30:30,600 --> 00:30:35,520
+serum و لبلازمة لنفس الشخص عملتلهم glucose test
+
+414
+00:30:35,520 --> 00:30:39,980
+راح ألاحظ أنه ال serum فيه قيمة السكر أقل من
+
+415
+00:30:39,980 --> 00:30:49,890
+البلازمة بكم؟ بنسبة 1.15%ليش؟ لإنه ال serum احتاج
+
+416
+00:30:49,890 --> 00:30:55,230
+إنه لازم الخلايا أو لازم الدم يتجلط بالأول و عشان
+
+417
+00:30:55,230 --> 00:31:01,510
+الدم يتجلط طبعا هذه عمليات .. عمليات التجلط و إنه
+
+418
+00:31:01,510 --> 00:31:05,970
+يتكون فيبرين و إنه الدم يمسك و يتكون و يعمل ال
+
+419
+00:31:05,970 --> 00:31:11,250
+clot هذه عمليات تتطلب إنتاج طاقة من الخلاياتمام؟
+
+420
+00:31:11,250 --> 00:31:15,090
+فعشان تنتج الخلايا هي الطاقة و تقوي تجلط الدم
+
+421
+00:31:15,090 --> 00:31:19,170
+بتكون ساحبت لها شوية glucose من ال serum تمام؟
+
+422
+00:31:19,170 --> 00:31:22,670
+عشان هيك دايما قيمة السيرا ال glucose .. السكر في
+
+423
+00:31:22,670 --> 00:31:28,140
+ال serum أقل منه في البلازمةال plasma لأ عادي يعني
+
+424
+00:31:28,140 --> 00:31:31,980
+أنا سحبت العين أو على طول روحت فصلتها و الدم لسه
+
+425
+00:31:31,980 --> 00:31:36,680
+تسائل و أخدت ال plasma اللي جمعتها من .. من فوق
+
+426
+00:31:36,680 --> 00:31:40,580
+الخلايا و فصلتها و خلاص يعني ما .. ما .. ماخلتش
+
+427
+00:31:40,580 --> 00:31:44,000
+احتكاك يعني بين ال plasma و الخلايا فترة طويلة لكن
+
+428
+00:31:44,000 --> 00:31:47,020
+لأ السيرم عشان أنا أفصله لازم الأول الدم يتجلط
+
+429
+00:31:47,020 --> 00:31:51,380
+بعدين أنا أفصله فعشان الدم يتجلط هادي بيكون إيش
+
+430
+00:31:51,380 --> 00:31:56,830
+استهلاك طاقةهيك بنكون وصلنا لنهاية محاضرتنا لليوم
+
+431
+00:31:56,830 --> 00:32:00,210
+أتمنى أنها تكون بسيطة وواضحة لإلكم ويعطيكم ألف
+
+432
+00:32:00,210 --> 00:32:00,690
+عافية
+
diff --git a/PL9fwy3NUQKwY9jl8xw-bWkVZkWcGGyspd/QS6fztFTb_Y.srt b/PL9fwy3NUQKwY9jl8xw-bWkVZkWcGGyspd/QS6fztFTb_Y.srt
new file mode 100644
index 0000000000000000000000000000000000000000..a008ca359eef76a215b818f5159d1e7e5122f6c9
--- /dev/null
+++ b/PL9fwy3NUQKwY9jl8xw-bWkVZkWcGGyspd/QS6fztFTb_Y.srt
@@ -0,0 +1,3215 @@
+1
+00:00:05,720 --> 00:00:09,280
+السلام عليكم ورحمة الله وبركاته. اليوم، إن شاء الله،
+
+2
+00:00:09,280 --> 00:00:14,000
+سنأخذ أول محاضرة نظرية في موضوع المقدمة في
+
+3
+00:00:14,000 --> 00:00:18,300
+التحليل الطبي أو مقدمة في المختبرات الطبية، إن شاء
+
+4
+00:00:18,300 --> 00:00:21,380
+الله. اليوم موضوعنا عن الـ medical laboratories، سن
+
+5
+00:00:21,380 --> 00:00:25,560
+نعرف ماذا يعني مختبر طبي، وما هي رسالة المختبر الطبي،
+
+6
+00:00:25,560 --> 00:00:30,240
+أو الهدف الذي يجب أن يحققه المختبر الطبي، وما هي
+
+7
+00:00:30,240 --> 00:00:33,520
+طبيعة الأعمال التي يقوم بها، وأنواع المختبرات
+
+8
+00:00:33,520 --> 00:00:38,350
+الطبية، بإذن الله. ولكن في البداية، يجب أن نعرج على
+
+9
+00:00:38,350 --> 00:00:44,630
+مصطلح اسمه health care provider، مزودو الصحة أو
+
+10
+00:00:44,630 --> 00:00:49,310
+العناية الصحية. ماذا يعني مزودو العناية الصحية؟ هذا
+
+11
+00:00:49,310 --> 00:00:54,530
+المصطلح يندرج تحته أي فرد، أي شخص،
+
+12
+00:00:54,530 --> 00:01:00,750
+أي مؤسسة، أي وكالة، أية جهة هي من شأنها أن تزود أو
+
+13
+00:01:00,750 --> 00:01:05,910
+تقدم health care أو health services لشخص
+
+14
+00:01:05,910 --> 00:01:09,310
+المستفيدين من الخدمات الصحية. يعني health care
+
+15
+00:01:09,310 --> 00:01:14,310
+provider، مزودو الرعاية الصحية، هو مصطلح يشمل أي
+
+16
+00:01:14,310 --> 00:01:19,510
+شخص، أي مؤسسة، أي وكالة تقدم رعاية صحية أو خدمات
+
+17
+00:01:19,510 --> 00:01:24,010
+صحية للناس الذين يعتبرون مستهلكين لهذه الخدمات
+
+18
+00:01:24,010 --> 00:01:32,280
+الصحية، الذين هم، بتعريف آخر، المرضى. ويندرج تحت هؤلاء
+
+19
+00:01:32,280 --> 00:01:36,500
+الأشخاص: الأطباء، الـ physicians، الـ
+
+20
+00:01:36,500 --> 00:01:42,020
+dentist، أطباء الأسنان، الممرضين، الأمراض، الذين
+
+21
+00:01:42,020 --> 00:01:46,740
+هم نحن، الـ medical laboratory scientists، تمام؟
+
+22
+00:01:46,740 --> 00:01:50,580
+medical laboratory scientists، الذين هم نحن، تمام؟ الـ
+
+23
+00:01:50,580 --> 00:01:56,890
+mental health counselors، الذين هم يعني، المزودين أو
+
+24
+00:01:56,890 --> 00:02:00,750
+يعني الاستشاريون حول الصحة النفسية والصحة العقلية،
+
+25
+00:02:00,750 --> 00:02:04,910
+الـ nursing، التمريض، وخدمات أخرى، يعني مثل التصوير
+
+26
+00:02:04,910 --> 00:02:11,650
+الطبي، والحكمة، والاختصاصات الأخرى لخدمات الصحية.
+
+27
+00:02:11,650 --> 00:02:15,470
+كلهم هؤلاء يندرجون تحت مصطلح health care provider.
+
+28
+00:02:16,090 --> 00:02:20,550
+في عصر التكنولوجيا الحديثة، تمام؟ أصبحنا... أصبحنا
+
+29
+00:02:20,550 --> 00:02:23,550
+محتاجين لكي نزود أو نعمل health care... health
+
+30
+00:02:23,550 --> 00:02:29,070
+care delivery، نزود أو نقدم خدمات صحية، لابد أن يكون
+
+31
+00:02:29,070 --> 00:02:32,970
+في عندي الكثير، أو so many different personnel، و
+
+32
+00:02:32,970 --> 00:02:36,530
+specialists. ماذا يعني هذا الكلام؟ زمان كان فقط في
+
+33
+00:02:36,530 --> 00:02:41,120
+طبيب، وفي حكيم، وخلاص، يعني ما... ما كان فيه لا
+
+34
+00:02:41,120 --> 00:02:46,480
+صيدلاني، ولا كان فيه مختبر، ولا من يعمل التصوير الطبي. هذه
+
+35
+00:02:46,480 --> 00:02:51,200
+التخصصات كلها نشأت حديثاً مع تقدم التكنولوجيا، أصبحنا
+
+36
+00:02:51,200 --> 00:02:55,200
+محتاجين many personnel، يعني أشخاص كثير، موظفين
+
+37
+00:02:55,200 --> 00:03:00,360
+كثير، بتخصصات مختلفة، حتى أيضاً أصبحنا محتاجين التخصّص
+
+38
+00:03:00,360 --> 00:03:03,520
+...في... في المؤسسات التي تقدم رعاية صحية،
+
+39
+00:03:03,520 --> 00:03:06,720
+أصبحنا محتاجين تخصصات ما لها علاقة، يعني الـ... الـ
+
+40
+00:03:06,720 --> 00:03:10,590
+...الـ medicine ليس هو الخلفية الخاصة بها، مثل على سبيل المثال الـ
+
+41
+00:03:10,590 --> 00:03:13,870
+secretariat، الإدارة والتدبير الصحي، والإدارة
+
+42
+00:03:13,870 --> 00:03:17,150
+التدبيرية. هذه كلها كانت... يعني الذين لهم علاقة
+
+43
+00:03:17,150 --> 00:03:22,250
+بأرشفة الملفات وحفظ الملفات واستخراجها، ونماذج
+
+44
+00:03:22,250 --> 00:03:28,450
+...الحفظ، نماذج التشخيص، ونماذج طلب الفحوصات،
+
+45
+00:03:28,450 --> 00:03:32,290
+هذا... هذا عملهم، تمام؟ ولكن أيضاً أصبحنا
+
+46
+00:03:32,290 --> 00:03:38,050
+محتاجين لهم في... منظومة تقديم الرعاية الصحية.
+
+47
+00:03:38,820 --> 00:03:42,560
+إذن، مع تقدم التكنولوجيا، أصبحنا محتاجين so many
+
+48
+00:03:42,560 --> 00:03:45,320
+different personnel and specialists. أصبحنا نعرف
+
+49
+00:03:45,320 --> 00:03:50,140
+ماذا تعني هذه الجملة، تمام؟ الـ caregivers، الناس الذين
+
+50
+00:03:50,140 --> 00:03:55,600
+يقدمون لي رعاية صحية. ما هي الشروط الواجب توفرها فيها؟ أول
+
+51
+00:03:55,600 --> 00:03:58,880
+شيء، لابد أن يكون لديها understanding and working
+
+52
+00:03:58,880 --> 00:04:02,740
+knowledge، يعني لديها معرفة، لديها background، خلفية
+
+53
+00:04:02,740 --> 00:04:09,510
+بإمكانها أن تقوم بتقديم diagnostic evaluation،
+
+54
+00:04:09,510 --> 00:04:14,790
+يعني تقييم وضع المريض، وتقييم تشخيصي، لكي تقدم رعاية
+
+55
+00:04:14,790 --> 00:04:18,850
+صحية صحيحة، تمام؟ ومن ضمن هؤلاء الناس الذين هم
+
+56
+00:04:18,850 --> 00:04:21,530
+caregivers، أو الـ care provider، health care
+
+57
+00:04:21,530 --> 00:04:24,680
+provider، الذين هم، كما قلنا، الـ medical laboratory
+
+58
+00:04:24,680 --> 00:04:29,640
+scientists. فبالتالي، الـ laboratory، والـ diagnostic
+
+59
+00:04:29,640 --> 00:04:33,460
+tests، فحوصات المختبر، والفحوصات التشخيصية التي تقام
+
+60
+00:04:33,460 --> 00:04:38,140
+أو تجرى داخل المختبر، هي عبارة عن أداة مهمة جداً لـ
+
+61
+00:04:38,140 --> 00:04:44,060
+health care delivery، لتقديم الرعاية الصحية. ولو أنها
+
+62
+00:04:44,060 --> 00:04:46,540
+...أنها هي هذه الخدمات التي نقدمها not
+
+63
+00:04:46,540 --> 00:04:49,460
+therapeutic. ماذا يعني not therapeutic؟ يعني أنا فقط
+
+64
+00:04:49,460 --> 00:04:52,740
+...أجري فحوصات طبية، أنا فعلياً ما أساهم في
+
+65
+00:04:52,740 --> 00:04:56,660
+علاج المريض بشكل مباشر، ولكن أساهم في علاج
+
+66
+00:04:56,660 --> 00:05:01,070
+المريض بشكل غير مباشر. كيف هذا الأمر يحدث؟ من خلال أن
+
+67
+00:05:01,070 --> 00:05:05,350
+مثلاً، المريض، الطبيب لديه الـ... الـ history،
+
+68
+00:05:05,350 --> 00:05:09,910
+التاريخ، أو الـ... الـ... الصورة العامة عن الـ...
+
+69
+00:05:09,910 --> 00:05:12,930
+تاريخ الـ... الـ... الـ patient، هل لديه أمراض
+
+70
+00:05:12,930 --> 00:05:16,650
+وراثية، هل هو يشكو من أمراض أو من أعراض سابقة، يعني
+
+71
+00:05:16,650 --> 00:05:20,630
+صورة... صورة المريض، أو الـ... الـ... الملف الـ... الـ
+
+72
+00:05:20,630 --> 00:05:25,960
+history الخاص به، تمام؟ سيرته المرضية، ويكون
+
+73
+00:05:25,960 --> 00:05:29,040
+أيضاً لديه physical examination. ماذا يعني
+
+74
+00:05:29,040 --> 00:05:33,600
+physical examination؟ يعني قام بـ... قام بفحصه، رأى
+
+75
+00:05:33,600 --> 00:05:39,540
+الأعراض، والـ... الـ... العلامات الظاهرة على المريض،
+
+76
+00:05:39,540 --> 00:05:44,100
+قام بفحصه، ورأى ما هو وضع المريض من الشكل الخارجي،
+
+77
+00:05:44,100 --> 00:05:51,240
+تمام؟ ونحن كـ MLS نقدم خدمات غير therapeutic، بل
+
+78
+00:05:51,240 --> 00:05:55,790
+يعني، بـ... بـ... بمساعدة الطبيب على التأكد، to
+
+79
+00:05:55,790 --> 00:06:00,650
+confirm diagnosis، تمام؟ ومن خلال أني أقدم value
+
+80
+00:06:00,650 --> 00:06:03,730
+بالـ information، معلومات قيّمة عن وضع المريض،
+
+81
+00:06:03,730 --> 00:06:09,210
+فبالتالي، الطبيب يستطيع أن يشخص بشكل صحيح، فيصف علاجاً صحيحاً، وبـ
+
+82
+00:06:09,210 --> 00:06:13,610
+التالي، يكون هناك response للـ therapy، المريض
+
+83
+00:06:13,610 --> 00:06:18,770
+ستتحسن حالته، الـ patient status ستتحسن، وأيضاً
+
+84
+00:06:18,770 --> 00:06:23,950
+سيكون هناك response للـ therapy. نلخص ماذا
+
+85
+00:06:23,950 --> 00:06:26,930
+الذي... الذي قلناه. قلنا إن مع
+
+86
+00:06:26,930 --> 00:06:31,010
+التكنولوجيا الحديثة، أصبحنا محتاجين تخصصات أكثر، عدد
+
+87
+00:06:31,010 --> 00:06:35,150
+موظفين أكثر. كان زمان المنشآت الصحية يقتصر عملها
+
+88
+00:06:35,150 --> 00:06:39,310
+على فقط ممرض وطبيب. الآن، أنتم في المنشأة الصحية طبعاً،
+
+89
+00:06:39,310 --> 00:06:42,690
+لديكم مثلاً، مثال مستشفى الشفاء، عدد مهول من
+
+90
+00:06:42,690 --> 00:06:48,670
+الموظفين، secretariat، إدارة صحية، إدارة طبية، أرشفة
+
+91
+00:06:48,670 --> 00:06:56,670
+ملفات، حتى موظفين للإشراف على الجودة، وضمان صحة
+
+92
+00:06:56,670 --> 00:07:01,530
+العمل، وجودة العمل. لديكم أطباء، أخصائيو مختبرات
+
+93
+00:07:01,530 --> 00:07:06,250
+طبية، صيادلة. إذن، عدد كبير من الـ personnel والـ
+
+94
+00:07:06,250 --> 00:07:11,670
+specialists، التخصصات أيضاً. وقلنا إن الشخص
+
+95
+00:07:11,670 --> 00:07:14,530
+الذي يقدم الرعاية الصحية، لابد أن يكون لديه
+
+96
+00:07:14,530 --> 00:07:17,410
+understanding... understanding and working
+
+97
+00:07:17,410 --> 00:07:21,410
+knowledge، يعني لابد أن يكون لديه خلفية، ويفهم الوضع
+
+98
+00:07:21,410 --> 00:07:28,710
+الصحي للمريض، أو الحالة الطبية التي تأتي
+
+99
+00:07:28,710 --> 00:07:32,410
+إليه، لكي يساعد في شيء اسمه diagnostic
+
+100
+00:07:32,410 --> 00:07:36,290
+evaluation، الذي هو يعني التقييم التشخيصي الصحي.
+
+101
+00:07:36,290 --> 00:07:41,930
+وقلنا أيضاً إن المختبرات، بغض النظر عن أنها هي لا
+
+102
+00:07:41,930 --> 00:07:46,310
+تقدم therapeutic approach، يعني لا تعطي، لا تساهم
+
+103
+00:07:46,310 --> 00:07:51,030
+مباشرة في العلاج، ولكن تساهم بطريقة غير مباشرة
+
+104
+00:07:51,030 --> 00:07:56,100
+في العلاج. كيف تساهم بطريقة غير مباشرة في العلاج؟ بأنها
+
+105
+00:07:56,100 --> 00:08:00,960
+تعمل diagnostic tests، تساهم، تساعد الطبيب على أن
+
+106
+00:08:00,960 --> 00:08:05,380
+يشخص، يؤكد الـ diagnosis الخاص به. من خلال، كيف يعني يؤكد
+
+107
+00:08:05,380 --> 00:08:09,700
+التشخيص الخاص به؟ من خلال ربط نتائج الفحوصات
+
+108
+00:08:09,700 --> 00:08:14,360
+الخاصة به مع الـ history الخاص بالمريض، ملف المريض، و
+
+109
+00:08:14,360 --> 00:08:18,940
+سيرته المرضية، مع الأعراض والعلامات الظاهرة
+
+110
+00:08:18,940 --> 00:08:22,040
+على المريض التي ظهرت من خلال الفحص physically،
+
+111
+00:08:22,040 --> 00:08:26,000
+وبالتالي، يكون لدينا معلومات قيّمة عن وضع المريض،
+
+112
+00:08:26,000 --> 00:08:32,440
+ومدى استجابته للعلاج. إذا أجريت الفحوصات بدقة، نتائج
+
+113
+00:08:32,440 --> 00:08:37,700
+صحيحة، تشخيص صحيح، وبالتالي علاج فعال. هذه هي السلسلة
+
+114
+00:08:37,700 --> 00:08:42,000
+التي يمر بها، خلال مرحلة الـ health care
+
+115
+00:08:42,000 --> 00:08:47,450
+providing، تمام؟ وأيضاً، نحن كمختبرات، لا نساعد فقط
+
+116
+00:08:47,450 --> 00:08:51,750
+الـ... الـ... الأطباء على أنهم هم يقومون بتأكيد
+
+117
+00:08:51,750 --> 00:08:57,110
+diagnosis، لا، نحن أيضاً نساعد في الـ epidemiology.
+
+118
+00:08:57,110 --> 00:09:01,930
+ماذا تعني الـ epidemiology؟ يعني مدى التقصي لمدى
+
+119
+00:09:01,930 --> 00:09:06,130
+انتشار الوباء. فعلياً، أنتم ترون مثلاً حالياً نحن
+
+120
+00:09:06,130 --> 00:09:11,980
+في... في ظل جائحة جائحة coronavirus، من الذي... الذي
+
+121
+00:09:11,980 --> 00:09:15,960
+على قدم وساق، ومن خلالهم نعرف عدد العينات التي
+
+122
+00:09:15,960 --> 00:09:20,120
+تُفحص، والـ positive results، كم عددهم، والـ
+
+123
+00:09:20,120 --> 00:09:24,340
+negative results. ومن الذي يقوم بهذا كله؟ من خلال الـ lab work،
+
+124
+00:09:24,340 --> 00:09:30,080
+نعرفه. فبالتالي، نحن نساهم من خلال المساعدة، أو
+
+125
+00:09:30,080 --> 00:09:37,210
+التقصي المتعلق بالوبائيات، وأيضاً research
+
+126
+00:09:37,210 --> 00:09:42,510
+purposes، أيضاً. المختبر له دور في أغراض البحث
+
+127
+00:09:42,510 --> 00:09:46,250
+العلمي. كيف يعني أغراض البحث العلمي؟ يعني من خلال
+
+128
+00:09:46,250 --> 00:09:51,450
+الدراسات والأبحاث التي تجرى داخل المختبرات. عندما نقول
+
+129
+00:09:51,450 --> 00:09:57,170
+مثلاً، والله، تم اكتشاف أن دواءً أو formula معين،
+
+130
+00:09:57,170 --> 00:10:04,150
+بصيغة معينة، أو مادة كيميائية معينة، مثلاً، لها دور
+
+131
+00:10:04,150 --> 00:10:08,420
+فعال في إذابة الجلطات، تمام؟ كيف اكتشفنا هذا؟
+
+132
+00:10:08,420 --> 00:10:12,680
+اكتشفناه من خلال أننا أجرينا عدة فحوصات، أو عدة
+
+133
+00:10:12,680 --> 00:10:18,000
+تجارب داخل المختبر، وأكدنا هذه العلاقة. فبالتالي،
+
+134
+00:10:18,000 --> 00:10:22,600
+أيضاً، الأبحاث المعتبرة، والنتائج، والاكتشافات العظيمة،
+
+135
+00:10:22,600 --> 00:10:28,580
+تخرج من المختبر. المختبر يؤكد الـ... الفحوصات، يساعد
+
+136
+00:10:28,580 --> 00:10:32,420
+في تقصي الحالات الوبائية، وظهور الـ... واكتشاف ظهور
+
+137
+00:10:32,420 --> 00:10:35,940
+الأمراض الجديدة، يساعد في إجراء الدراسات العلمية،
+
+138
+00:10:35,940 --> 00:10:40,600
+والتقدم العلمي. المختبر، أو أخصائي المختبر، هو عين
+
+139
+00:10:40,600 --> 00:10:46,020
+الطبيب الثانية. الطبيب له عين ثانية، عين ترى الـ
+
+140
+00:10:46,020 --> 00:10:49,180
+physical examination، التي هي العلامات الظاهرة
+
+141
+00:10:49,180 --> 00:10:55,000
+خارجياً على المريض، وطبعاً، هذه العلامات طبعاً متداخلة في
+
+142
+00:10:55,000 --> 00:10:58,020
+كل... في معظم الأمراض. ماذا يعني متداخلة في معظم
+
+143
+00:10:58,020 --> 00:11:01,700
+الأمراض؟ يعني مثلاً، الأعراض التي تظهر على مريض
+
+144
+00:11:01,700 --> 00:11:10,610
+معين، هي عبارة عن حمى، ألم في العضلات والعظام، يعني
+
+145
+00:11:10,610 --> 00:11:18,090
+مثلاً، قد يكون هناك diarrhea. هذه العلامات الثلاث، كثير
+
+146
+00:11:18,090 --> 00:11:22,550
+من الأمراض يكون من أعراضها وجود حمى، وإسهال، وألم في
+
+147
+00:11:22,550 --> 00:11:27,730
+العضلات والمفاصل أو في العظام، وأيضاً ارتفاع في درجة
+
+148
+00:11:27,730 --> 00:11:32,030
+الحرارة: الـ influenza، والرشح، والتهابات أو نزلات
+
+149
+00:11:32,030 --> 00:11:37,740
+المعوية، وكثير من الأمراض، حتى COVID-19، من هذه العلامات
+
+150
+00:11:37,740 --> 00:11:41,840
+من أعراضه. إذن، المريض لن... الطبيب لن... لن
+
+151
+00:11:41,840 --> 00:11:48,980
+يستطيع حصر الـ... الـ... الأمراض التي قد يخمنها،
+
+152
+00:11:48,980 --> 00:11:54,300
+وتفسيرها، أو تشخيصها، وماذا يعاني منه المريض. إلا إذا نحن
+
+153
+00:11:54,300 --> 00:11:58,820
+قمنا بإجراء فحوصات، وقلنا له، والله، إن هذا المريض،
+
+154
+00:11:58,820 --> 00:12:04,560
+الذي لديه حمى، وألم في العضلات والعظام، ولديه أيضاً
+
+155
+00:12:04,560 --> 00:12:12,100
+diarrhea، مثلاً، هو لديه، مثلاً، طفل معين في القمعة، وجود
+
+156
+00:12:12,100 --> 00:12:15,180
+...اكتشفنا وجود طفل معين في القمعة، إذن، سيذهب
+
+157
+00:12:15,180 --> 00:12:19,990
+تفكير المريض... تفكير الطبيب، أن هذا المريض يعاني من
+
+158
+00:12:19,990 --> 00:12:25,150
+نازلة معوية، ليس من virus، ولا influenza، ولا رشح، ولا
+
+159
+00:12:25,150 --> 00:12:29,850
+أي شيء آخر. إذن، الطبيب بعينه رأى العلامات
+
+160
+00:12:29,850 --> 00:12:34,690
+الخارجية التي ظهرت على المريض، ونحن بالعين
+
+161
+00:12:34,690 --> 00:12:40,810
+الثانية للطبيب، رأينا العلامات الداخلية، استكشفنا داخل
+
+162
+00:12:40,810 --> 00:12:44,970
+المريض ما به، من خلال أننا فحصنا عيناته، و
+
+163
+00:12:44,970 --> 00:12:49,170
+أكدنا الـ diagnosis. إذن، دورنا لا يقل أهمية عن
+
+164
+00:12:49,170 --> 00:12:54,310
+الطبيب في التشخيص، تمام؟ نحن نساعد، ونساند، وعين
+
+165
+00:12:54,310 --> 00:12:58,600
+الطبيب الثانية. طيب، introduction to medical
+
+166
+00:12:58,600 --> 00:13:01,340
+laboratory technology. ماذا تعني مقدمة في التحاليل
+
+167
+00:13:01,340 --> 00:13:07,360
+الطبية؟ هو عبارة... قلنا هو basic course، مساق تأسيسي،
+
+168
+00:13:07,360 --> 00:13:11,420
+يساعد الطلاب على أن يأخذوا الـ essential
+
+169
+00:13:11,420 --> 00:13:15,780
+knowledge، المعرفة الأساسية، والمهارات الأساسية،
+
+170
+00:13:15,780 --> 00:13:20,400
+لكي يعرفوا كيف يتصرفون داخل المختبرات الطبية، و
+
+171
+00:13:20,400 --> 00:13:25,440
+هذا الكلام طبعاً، ذكرناه في محاضرة التقديم. طبعاً،
+
+172
+00:13:25,440 --> 00:13:30,280
+ما هي أهمية المختبرات الطبية، أو أهمية المساق؟ طبعاً،
+
+173
+00:13:30,280 --> 00:13:37,400
+سيتعلمنا أهمية، أو مدى أهمية الخدمات التي يقدمها
+
+17
+
+223
+00:17:23,890 --> 00:17:28,590
+آجي أشيك على درجة حرارة الثلاجة، أنْها هي ده يعني
+
+224
+00:17:28,590 --> 00:17:33,520
+درجة حرارتها مضبوطة تمام؟ أنا لازم عشان أنمّي
+
+225
+00:17:33,520 --> 00:17:37,480
+بكتيريا، وأتعرف عليها، وأشخص الـ ... نوع البكتيريا
+
+226
+00:17:37,480 --> 00:17:42,580
+اللي عاملة مرض لمريض معيّن، لازم أنمّي هذه البكتيريا
+
+227
+00:17:42,580 --> 00:17:47,140
+على درجة حرارة ٣٧، إذا جهاز الـ incubator الحاضنة
+
+228
+00:17:47,140 --> 00:17:50,120
+اللي أنا بدي أنمّي فيه البكتيريا هذه، كل يوم بدي
+
+229
+00:17:50,120 --> 00:17:54,870
+أشيك عليه، وأتأكد أنْ درجة حرارته ٣٧، إذا أنا بدي
+
+230
+00:17:54,870 --> 00:17:59,850
+أشتغل، بدي أفصل عينات، مثلاً بدي أفصلها على جهاز
+
+231
+00:17:59,850 --> 00:18:07,650
+الجهاز هذا طبعاً إله عدد لفات معيّنة في الدقيقة
+
+232
+00:18:07,650 --> 00:18:11,830
+الواحدة، بيشتغل على الكهربا، إذا لازم أشيك على التيار
+
+233
+00:18:11,830 --> 00:18:16,920
+الكهربائي اللي بيجي لعنده، شغله لازم يكون فيه، الجهاز
+
+234
+00:18:16,920 --> 00:18:22,120
+هذا يكون موزون، أحقق الـ balance تبعه، إذا لما أنا
+
+235
+00:18:22,120 --> 00:18:25,900
+أكون متأكدة أن كل الأجهزة، وكل الأدوات تبعتي
+
+236
+00:18:25,900 --> 00:18:31,100
+مضبوطة، إذا أنا وفّرت controlled conditions، ظروف
+
+237
+00:18:31,100 --> 00:18:35,360
+مضبوطة، لحتى أشتغل scientific research، تجارب
+
+238
+00:18:35,360 --> 00:18:39,880
+experiments، وقياسات measurements، وتكون كلها
+
+239
+00:18:39,880 --> 00:18:46,340
+أشْمالها، نتائجها تعتمد، تمام؟ لكن مثلاً لما تكون الثلاجة
+
+240
+00:18:46,340 --> 00:18:49,940
+مرة بتفصل، مرة بترتفع، طب أنا مش متأكدة أن
+
+241
+00:18:49,940 --> 00:18:53,120
+الـ reagent هذا، أو المادة الكيميائية اللي موجودة في
+
+242
+00:18:53,120 --> 00:18:57,500
+الظروف فعّالة، ولا خربت، درجة الحرارة مش ثابتة، معناه
+
+243
+00:18:57,500 --> 00:19:01,440
+أنا مش هتأكد، الفحص تبعي، أو التجربة تبعتي اللي بدي
+
+244
+00:19:01,440 --> 00:19:04,760
+أشتغلها باستخدام المادة الكيميائية هذه، هتعطيني
+
+245
+00:19:04,760 --> 00:19:09,360
+نتائج دقيقة، فبالتالي، هذا معنى كلمة controlled
+
+246
+00:19:09,360 --> 00:19:13,240
+conditions، كل شيء داخل المختبر يجب أن يكون مضبوط،
+
+247
+00:19:13,240 --> 00:19:19,480
+نشيك عليه، نتأكد من ... من أنْ هو بيشتغل في الظروف
+
+248
+00:19:19,480 --> 00:19:23,980
+اللي لازم يكون موجود عليها، طبعاً medical laboratory
+
+249
+00:19:23,980 --> 00:19:28,390
+طبعاً laboratory هي عبارة عن كلمة أو مصطلح عام،
+
+250
+00:19:28,390 --> 00:19:32,210
+medical laboratory هو مصطلح خاص، ونفس المعنى،
+
+251
+00:19:32,210 --> 00:19:37,310
+clinical laboratory، مختبر طبي، أو مختبر سريري هو
+
+252
+00:19:37,310 --> 00:19:41,330
+عبارة عن المختبر، أو المنشأة، تمام، المختبر، أو
+
+253
+00:19:41,330 --> 00:19:46,330
+المنشأة اللي من خلالها أنا أش... بشتغل، أش نوعية
+
+254
+00:19:46,330 --> 00:19:49,770
+التجارب اللي بدي اشتغلها، بدي اشتغلها على clinical
+
+255
+00:19:49,770 --> 00:19:54,630
+specimen، تمام؟ ومن خلالها أنا بدي آخذ نتائج، get
+
+256
+00:19:54,630 --> 00:19:58,810
+information، علشان تساعدني في الـ diagnosis، ومن ثمّ لـ
+
+257
+00:19:58,810 --> 00:20:02,410
+treatment، وايضاً لـ prevention of disease، أمنع
+
+258
+00:20:02,410 --> 00:20:05,170
+انتشار الأمراض، من خلال أنْ أنا أحقق الـ diagnosis
+
+259
+00:20:05,170 --> 00:20:09,470
+الصحية، treatment صحية، فبالتالي أُحدّ من انتشار الأمراض
+
+260
+00:20:09,470 --> 00:20:13,580
+بهذا الشكل، طيب إيش الفرق ما بين laboratory و
+
+261
+00:20:13,580 --> 00:20:16,020
+medical laboratory؟ حكينا laboratory هي الكلمة
+
+262
+00:20:16,020 --> 00:20:20,840
+العامة، أو التعريف العام لمصطلح مختبر، طبعاً أنتم
+
+263
+00:20:20,840 --> 00:20:28,600
+يمكن ارتدّتوا، أو شفتوا مبنى لمختبرات، كان مكوّن من
+
+264
+00:20:28,600 --> 00:20:32,240
+عدّة طوابق، الفيزياء عندهم مختبرات، الكيمياء عندهم
+
+265
+00:20:32,240 --> 00:20:35,960
+مختبرات، إحنا عندنا في العلوم الطبية المخبرية
+
+266
+00:20:35,960 --> 00:20:40,340
+عندنا مختبرات، وحتى في علوم الأرض والبيئة عندهم
+
+267
+00:20:40,340 --> 00:20:44,160
+مختبرات، ولكن طبعاً كلهم نفس الشيء، عندهم control
+
+268
+00:20:44,160 --> 00:20:47,360
+conditions، وبيشتغلوا research، وبيشتغلوا experiments
+
+269
+00:20:47,360 --> 00:20:52,140
+و measurements، ولكن نوعية الدراسات، أو نوعية الـ
+
+270
+00:20:52,140 --> 00:20:54,800
+measurements، والفحوصات، والـ experiment، والتجارب
+
+271
+00:20:54,800 --> 00:20:59,380
+اللي بيشتغلوها تختلف، تمام؟ مثلاً بالكيمياء بيجيبوا
+
+272
+00:20:59,380 --> 00:21:02,260
+مادة unknown، مش معروفة، بيجروا عليها فحوصات
+
+273
+00:21:02,260 --> 00:21:04,940
+كيميائية معيّنة، بيتعرفوا على مثلاً الـ molecular
+
+274
+00:21:04,940 --> 00:21:10,020
+weight تبعتها، بيتعرفوا على خصائصها، هي Redox ولا ...
+
+275
+00:21:11,550 --> 00:21:15,410
+مثلاً Reductant أو Oxidant، بتعرفوا عليها أنها ...
+
+276
+00:21:15,410 --> 00:21:18,810
+يعني بالنهاية بياخدوا صورة كاملة عن المادة، وممكن
+
+277
+00:21:18,810 --> 00:21:21,390
+يعرفوها أنها هذه المادة، أو هذا المحلول، أو هذه
+
+278
+00:21:21,390 --> 00:21:28,030
+الصيغة ... صيغتها الكيميائية تصير معروفة، ال... الأرض
+
+279
+00:21:28,030 --> 00:21:33,070
+والبيئة بيجروا فحوصات، وأبحاث، وقياسات، ولكن ممكن
+
+280
+00:21:33,070 --> 00:21:38,190
+تكون على عينات تربة، عينات ماء، عينات صخور، يعني هيك
+
+281
+00:21:38,190 --> 00:21:41,950
+... هيك علم الأرض والبيئة، لكن إحنا الـ medical
+
+282
+00:21:41,950 --> 00:21:47,170
+laboratory، بتخصصنا، بنجري فحوصات tests، بنشــتري
+
+283
+00:21:47,170 --> 00:21:51,310
+فحوصات على إيش؟ على clinical specimen، إيش يعني
+
+284
+00:21:51,310 --> 00:21:56,070
+clinical specimen؟ عينة مرضية، أو سريرية، يعني عينة
+
+285
+00:21:56,070 --> 00:22:00,530
+أنا أخدتها من شخص، هو suspected، شك فيه أنه يكون
+
+286
+00:22:00,530 --> 00:22:04,310
+مريض، طبعاً مش ... أنا بجزمش أنه يكون مريض، لكن شك فيه
+
+287
+00:22:04,310 --> 00:22:09,270
+أنه يكون مريض، تمام؟ فبالتالي أي عينة تُؤخذ من شخص
+
+288
+00:22:09,270 --> 00:22:13,490
+suspected أنه يكون مريض، فبالتالي بسمّيها clinical
+
+289
+00:22:13,490 --> 00:22:18,210
+sample، عينة الـ ... الـ urine، الـ bowel، عينة الدم،
+
+290
+00:22:18,210 --> 00:22:22,270
+عينة الـ ... حتى الـ ... الـ ... الشعر، الأظافر، هذه
+
+291
+00:22:22,270 --> 00:22:27,730
+كلها عينات، سواء الجسم، الأنسجة، هذه كلها عينات
+
+292
+00:22:27,730 --> 00:22:30,770
+إحنا بناخدها من ناس شكّاكين فيهم إن هم يكونوا مرضى، أو
+
+293
+00:22:30,770 --> 00:22:35,480
+لأ، والفحوصات تثبت، أو تنفي مرضهم، تمام؟ فبالتالي
+
+294
+00:22:35,480 --> 00:22:39,880
+هذه بيسمّوها ... كلها clinical samples، تمام؟ إيش
+
+295
+00:22:39,880 --> 00:22:43,820
+يعني medical laboratory science؟ إيش يعني تخصصنا؟
+
+296
+00:22:43,820 --> 00:22:49,020
+تخصصنا هو أنْ أنا أوظّف، أو أستخدم الـ clinical
+
+297
+00:22:49,020 --> 00:22:54,780
+laboratory، علشان إيش أعمل؟ أكتشف، أشخص، أراقب، و
+
+298
+00:22:54,780 --> 00:22:59,660
+أعالج المرض، تمام؟ detect، بدي أشوف هذا الشخص مريض
+
+299
+00:22:59,660 --> 00:23:04,920
+ولا لأ، وبالتالي أ... أ... أعمله diagnosis، وأراقب
+
+300
+00:23:04,920 --> 00:23:09,320
+حالة المريض، أحياناً بيجيني مريض، ممكن أطلب، يطلع يعني
+
+301
+00:23:09,320 --> 00:23:12,820
+الدكتور يوجّه له عندي، أعمله فحص في بداية الخطة
+
+302
+00:23:12,820 --> 00:23:16,880
+العلاجية، في نصف الخطة العلاجية، برضه كمان أجري له فحص
+
+303
+00:23:16,880 --> 00:23:20,720
+ثاني، عشان أشوفه تحسّن، ولا ما تحسّنش، طبعاً وفي نهاية
+
+304
+00:23:20,720 --> 00:23:23,040
+الخطة العلاجية، ممكن أجري له فحص، هذا بسمّيه
+
+305
+00:23:23,040 --> 00:23:27,800
+monitoring، مراقبة، طبعاً، وايضاً علشان نقدر إحنا
+
+306
+00:23:27,800 --> 00:23:33,060
+نساهم indirectly، بعلاج المرض، طبعاً إحنا حكينا أنا
+
+307
+00:23:33,060 --> 00:23:37,380
+ما بساهم بشكل direct، ولكن بشكل indirect، طبعاً إيش
+
+308
+00:23:37,380 --> 00:23:39,860
+نوع العينات اللي بتيجيني في الـ clinical laboratory؟
+
+309
+00:23:39,860 --> 00:23:43,860
+قلنا clinical samples، أو clinical specimen، طبعاً
+
+310
+00:23:43,860 --> 00:23:48,380
+clinical specimen، أو clinical samples، نفس المعنى،
+
+311
+00:23:48,380 --> 00:23:55,410
+طبعاً، طيب إيش يعني؟ ممكن تكون دم blood، tissue، تمام،
+
+312
+00:23:55,410 --> 00:23:59,050
+body fluid، سواء الجسم، ممكن تكون دم أنتجته سواء
+
+313
+00:23:59,050 --> 00:24:03,470
+الجسم، أي نوع من ... أي عينة تؤخذ من شخص suspected
+
+314
+00:24:03,470 --> 00:24:07,050
+أنه يكون مريض، بسمّيها clinical samples، طبعاً بحلّلها
+
+315
+00:24:07,050 --> 00:24:11,000
+كيميائياً، بعمل لها chemical analysis، و examination
+
+316
+00:24:11,000 --> 00:24:14,980
+علشان أشوف والله هذا الشخص عنده foreign organism،
+
+317
+00:24:14,980 --> 00:24:20,080
+عنده مثلاً جسم غريب، أو كائن غريب، زي بكتيريا، فطريات،
+
+318
+00:24:20,080 --> 00:24:24,740
+فيروسات، عملت له عدوى، واللي عنده مثلاً abnormalities،
+
+319
+00:24:24,740 --> 00:24:30,260
+وظائف كبد، وظائف كلية، وظائف قلب، وظائف الأعضاء
+
+320
+00:24:30,260 --> 00:24:34,060
+كلها، أشوفها طبيعية، ولا abnormal، normal، ولا
+
+321
+00:24:34,060 --> 00:24:39,210
+abnormal، تمام؟ هذا برضه كمان من ضمن شغلنا كأخصائي
+
+322
+00:24:39,210 --> 00:24:44,250
+مختبرات طبية، كمان المعلومات اللي إحنا حكينا بت...
+
+323
+00:24:44,250 --> 00:24:49,250
+اللي بنستخدمها، بتساعد الفريق الطبي، عشان يأخذ
+
+324
+00:24:49,250 --> 00:24:55,690
+قرارات تبع هذا المريض، بتساعد طبعاً، أنا لما بقدّم
+
+325
+00:24:55,690 --> 00:25:00,430
+نتائج الفحوصات، الطبيب بيصير... تبقى... بيربط
+
+326
+00:25:00,430 --> 00:25:03,350
+النتائج تبعتي، حكينا بالـ history تبعت المريض،
+
+327
+00:25:03,350 --> 00:25:06,210
+بالعلامات، والـ physical examination اللي ظاهرة على
+
+328
+00:25:06,210 --> 00:25:10,690
+المريض، وبالتالي بياخذ قرار، أن هذا المريض مُشخص بمرض
+
+329
+00:25:10,690 --> 00:25:16,920
+كذا، ويسرّف له العلاج كذا، فبالتالي، وبرسم له الخطة
+
+330
+00:25:16,920 --> 00:25:21,820
+العلاجية، طبعاً ٨٥٪ من القرارات
+
+331
+00:25:21,820 --> 00:25:26,940
+الطبية تعتمد على إيش؟ على الـ ... الـ ... النتائج
+
+332
+00:25:26,940 --> 00:25:31,380
+المختبر تبعتي، أو نتائج البحوث تبعتي، إذا أنا العين
+
+333
+00:25:31,380 --> 00:25:35,640
+العين، الطبيب الثاني اللي بتشوف بنسبة ٨٥٪
+
+334
+00:25:35,640 --> 00:25:40,480
+في المئة، أما عين الطبيب alone بتشوف بنسبة ١٥٪، إذا
+
+335
+00:25:40,480 --> 00:25:45,020
+لا تستهينوا بتخصصكم، احبّوا تخصصكم، احبّوا الشيء اللي
+
+336
+00:25:45,020 --> 00:25:49,680
+أنتم جايين تتعلموه، ويكونوا فخورين بما تصنعون، لأنّ
+
+337
+00:25:49,680 --> 00:25:55,080
+أنتم تخصصكم لا يُستهان فيه، ولا يمكن الاستغناء عنه،
+
+338
+00:25:55,450 --> 00:26:02,870
+يعني حتى الأطباء، والصيادلة، والأطباء لما بدهم ييجوا
+
+339
+00:26:02,870 --> 00:26:06,710
+يعملوا دراسات عليا، لابد من أنهم يقوموا بإجراء
+
+340
+00:26:06,710 --> 00:26:12,320
+فحوصات، وتجارب داخل مختبراتنا، تمام؟ فبالتالي يعني
+
+341
+00:26:12,320 --> 00:26:18,580
+إحنا تخصصنا كثير مهم، وهذا يعني أنه لا يقل أهمية
+
+342
+00:26:18,580 --> 00:26:22,180
+عن التخصصات الأخرى، أنا لا أقول أن التخصصات الأخرى
+
+343
+00:26:22,180 --> 00:26:26,620
+غير مهمة، هي مهمة، وهي حجر أساس، ولكن أيضاً تخصصنا
+
+344
+00:26:26,620 --> 00:26:32,040
+مهم جداً، ويجب أن أنتم تحبّوا، وتفتخروا فيه، وتحسّوا
+
+345
+00:26:32,040 --> 00:26:38,180
+بقدْره، أنتم رح يكون دوركم فعّال، وقيم في عملية الـ
+
+346
+00:26:38,180 --> 00:26:43,400
+health care providing، عمليات تزويد الخدمات الصحية
+
+347
+00:26:43,400 --> 00:26:50,740
+لمستحقيها، أو مستهلكيها، what are lab tests؟ إيش
+
+348
+00:26:50,740 --> 00:26:55,600
+يعني الفحوصات الطبية؟ الفحوصات الطبية هي عبارة عن
+
+349
+00:26:55,600 --> 00:26:59,060
+الـ medical procedures، التجارب الطبية اللي من
+
+350
+00:26:59,060 --> 00:27:02,180
+خلالها اللي بنبدأ نطلب الحصول على عينة clinical
+
+351
+00:27:02,180 --> 00:27:05,920
+sample، أو clinical specimen، اللي هي عبارة عن blood،
+
+352
+00:27:05,920 --> 00:27:10,090
+أو urine، أو other tissue، تمام؟ إذا الـ ... الـ ...
+
+353
+00:27:10,090 --> 00:27:13,630
+المصطلحات، بتلاحظوا إنها مرتبطة في بعضها البعض، و
+
+354
+00:27:13,630 --> 00:27:17,030
+تندرج تحت بعض، يعني بنعرف إيش يعني تخصص lab، بنصير
+
+355
+00:27:17,030 --> 00:27:19,870
+نفهم إيش يعني clinical lab، أو medical lab، بنصير
+
+356
+00:27:19,870 --> 00:27:23,810
+نفهم إيش يعني lab test، وإيش يعني clinical sample،
+
+357
+00:27:23,810 --> 00:27:29,250
+فكل هذه يعني معلومات مترابطة مع بعضها البعض، طيب إيش
+
+358
+00:27:29,250 --> 00:27:33,930
+الدكتور بيستفيد من نتائج المختبر؟ طبعاً الدكتور
+
+359
+00:27:33,930 --> 00:27:41,890
+دوره أنه يلاحظ، يعرف التغيرات اللي حاصلة على صحة
+
+360
+00:27:41,890 --> 00:27:46,450
+المريض، طبعاً ممكن خلال، أو بعد، أو قبل ظهور الـ
+
+361
+00:27:46,450 --> 00:27:52,270
+symptoms، الأعراض، من خلال نتائج المختبر، أيضاً بتساعد
+
+362
+00:27:52,270 --> 00:27:57,070
+نتائج المختبر، أو الـ laboratory test، بتساعد الطبيب
+
+363
+00:27:57,070 --> 00:28:02,950
+أنه يعمل diagnoses، يشخص المرض، يرسم خطة علاجية plan،
+
+364
+00:28:02,950 --> 00:28:09,670
+patient treatment، وايضاً يقيم وضع المريض، هل استجاب
+
+365
+00:28:09,670 --> 00:28:14,190
+هل صار في عنده response، أو لأ، للـ treatment، وايضاً
+
+366
+00:28:14,190 --> 00:28:19,350
+يراقب وضع المريض، هل الحالة بتستقر، أو بتتحسّن، وهذا
+
+367
+00:28:19,350 --> 00:28:25,430
+الكلام ملخّص في الجملة، هذا الكلام كله، هذا ملخّص في
+
+368
+00:28:25,430 --> 00:28:30,130
+الجملة اللي إحنا حكيناها، detect, diagnose, monitor،
+
+369
+00:28:30,130 --> 00:28:36,170
+and treat، طبعاً إذا أنظروا detect، بيكتشف إيش
+
+370
+00:28:36,170 --> 00:28:40,670
+التغيرات اللي حدثت على صحة المريض، diagnose، بيشخص، بي
+
+371
+00:28:40,670 --> 00:28:45,870
+treat، بيعالج، وايضاً بيعمل monitoring، و evaluation
+
+372
+00:28:45,870 --> 00:28:53,570
+لحالة المريض، طيب نتائج المختبر، أو نتائج الفحوصات
+
+373
+00:28:53,570 --> 00:28:59,710
+الطبية، طبعاً فيه عدّة عوامل بتأثّر عليها، زي age، مثلاً
+
+374
+00:28:59,710 --> 00:29:05,480
+زي الـ gender، الـ gender، الـ sex، والـ age، تمام؟ إيش
+
+375
+00:29:05,480 --> 00:29:11,180
+يعني gender؟ يعني بتختلف؟ كيف يعني الـ gender بتختلف
+
+376
+00:29:11,180 --> 00:29:17,700
+نتائج الفحوصات؟ طبعاً لإله؟ مثلاً إحنا كإناث معروف
+
+377
+00:29:17,700 --> 00:29:23,160
+أن نسبة الـ hemoglobin عندنا في الدم من ١١ ل ١٣، لكن
+
+378
+00:29:23,160 --> 00:29:27,820
+الـ ... الـ ... الذكور، الـ males، عندهم نسبة الـ
+
+379
+00:29:27,820 --> 00:29:35,760
+hemoglobin، تمام، من ١٤ ل ١٧، أو ل ١٧، طبعاً
+
+380
+00:29:35,760 --> 00:29:40,940
+هم عندهم نسبة hemoglobin أعلى، طب ليش؟ لأنّ فيه فروق
+
+381
+00:29:40,940 --> 00:29:45,380
+تبعاً للـ sex، أو الـ gender، طبعاً ... طب ... إحنا كـ
+
+382
+00:29:45,380 --> 00:29:50,780
+كإناث، إحنا كل يوم ... كل شهر عندنا مقدار loss، مقدار
+
+383
+00:29:50,780 --> 00:29:54,840
+فقدان من الدم، من خلال الـ menstruation، تمام؟ وعندنا أيضاً الـ ... الـ muscle mass، الكتلة العضلية
+
+384
+00:29:54,840 --> 00:29:58,960
+عندنا أقل منهم، فبالتالي هيكون عندنا نسبة الـ
+
+385
+00:29:58,960 --> 00:30:03,310
+hemoglobin أقل، يعني إحنا لأنّ بنفقد blood بشكل
+
+386
+00:30:09,370 --> 00:30:14,550
+شهري، ولأنّ عندي عضلات أقل، فأنا محتاجة hemoglobin
+
+387
+00:30:14,550 --> 00:30:19,030
+أو بيكون عندي الـ hemoglobin أقل، هم عندهم الـ body
+
+388
+00:30:19,030 --> 00:3
+
+445
+00:34:37,500 --> 00:34:42,660
+قبل إجراء الفحص، وبتأثر نتائج الفحصات بنوع الأدوية
+
+446
+00:34:42,660 --> 00:34:47,340
+اللي بياخدها المريض قبل إجراء الفحص، وأيضا جدّا هوش
+
+447
+00:34:47,340 --> 00:34:51,240
+التابع للتعليمات. طبعًا في بحوث بيجي المريض أنا
+
+448
+00:34:51,240 --> 00:34:55,620
+بشرح له التعليمات كيف هو يجي.. مثلا في.. في عينات
+
+449
+00:34:55,620 --> 00:34:58,820
+أنا بجمعها من المريض، يعني أنا مثلا باخد منه عينة
+
+450
+00:34:58,820 --> 00:35:03,960
+الدم، أنا باخد يعني، أو طبيب كمان ممكن ياخد منه
+
+451
+00:35:03,960 --> 00:35:11,600
+عينات سواء من الجسم، أو عينات الأنسجة، لكن في عينات ال
+
+452
+00:35:11,600 --> 00:35:15,240
+... المريض بنفسه بيجمعها، فبالتالي أنا لازم أعطي
+
+453
+00:35:15,240 --> 00:35:18,360
+تعليمات كيف يجمعها بشكل صحيح، عشان أنا أجري فحص و
+
+454
+00:35:18,360 --> 00:35:23,460
+آخذ نتائج صحيحة، وبالتالي تكون النتائج فعّالة.
+
+455
+00:35:23,880 --> 00:35:28,380
+فبالتالي أيضا جدّا هو التزم واتبع ال... ال
+
+456
+00:35:28,380 --> 00:35:34,060
+pre-examination instructions التعليمات ما قبل الفحص، بي...
+
+457
+00:35:34,060 --> 00:35:38,360
+بتكون الفحص ناجحًا. التعليمات هذه كمان مش بس بيجمع
+
+458
+00:35:38,360 --> 00:35:42,220
+العينات، يعني كيف هو يجمع العينة، لأ، برضه كمان بال
+
+459
+00:35:42,220 --> 00:35:45,980
+... بالمعلومات السابقة، يعني مثلا ما يأخذش مضادات
+
+460
+00:35:45,980 --> 00:35:49,280
+حيوية قبل إجراء الفحص، إذا هو التزم، أيضا الفحص
+
+461
+00:35:49,280 --> 00:35:53,580
+هينجح. ما يأكلش، ما يشربش، تعال صائمًا مثلا من 6 ل 8
+
+462
+00:35:53,580 --> 00:35:58,840
+ساعات. أنواع معينة من الأكل لازم تتجنبها قبل إجراء
+
+463
+00:35:58,840 --> 00:36:04,200
+الفحص. في فحوصات لازم يأكل شيء معين قبل إجراءه، يعني
+
+464
+00:36:04,200 --> 00:36:10,840
+مثلا أحيانا عشان نَشخص مشاكل الإنسولين، بعطي أنا
+
+465
+00:36:10,840 --> 00:36:15,780
+محلول سكري بخليه يشربه، وبجري الفحص بعد نص ساعة
+
+466
+00:36:15,780 --> 00:36:22,400
+منه، وبعد ساعة، وبعد ساعتين. فإذًا التعليمات قبل إجراء
+
+467
+00:36:22,400 --> 00:36:30,320
+الفحص مهمة جدًا، وعلى أساسها بتختلف أو بتتأثر نتائج
+
+468
+00:36:30,320 --> 00:36:38,020
+الفحصّات. طبعًا ال... ال... نتائج المختبر أو فحوصات
+
+469
+00:36:38,020 --> 00:36:44,960
+المختبر بت... بتورجيني قدّاش الشخص هذا normal أو
+
+470
+00:36:44,960 --> 00:36:50,000
+abnormal. يعني نتائجه إذا وقعت داخل الـ normal range
+
+471
+00:36:50,000 --> 00:36:54,240
+معناها هو شخص normal، أما إذا وقعت النتائج خارج الـ
+
+472
+00:36:54,240 --> 00:36:58,040
+normal range، أقل أو أعلى، فهو شخص abnormal، عنده
+
+473
+00:36:58,040 --> 00:37:03,460
+مشاكل ويجب أن تعالج. طبعًا النتائج المختبر ما بعطيهاش
+
+474
+00:37:03,460 --> 00:37:08,660
+قيمة، والله مثلا نسبة الـ hemoglobin الطبيعية 11 لأ
+
+475
+00:37:08,660 --> 00:37:12,060
+غلط، أحكي هيك: نسبة الـ hemoglobin الطبيعية عند
+
+476
+00:37:12,060 --> 00:37:15,920
+الإناث من 11 ل 13، وعند الرجال من 14 ل 16، أو من
+
+477
+00:37:15,920 --> 00:37:21,000
+14 ل 17. هذا الكلام الصحيح، لإن أنا ممكن يكون دمي 11
+
+478
+00:37:21,000 --> 00:37:24,800
+وزميلتي 11.5، وزميلتي الثانية 12، وزميلتي
+
+479
+00:37:24,800 --> 00:37:29,940
+الثالثة 13، وكلنا normal. فبالتالي فيه فروق فردية
+
+480
+00:37:29,940 --> 00:37:34,840
+من شخص لآخر، من حسب الكتلة الجسمية تبعته، حسب
+
+481
+00:37:34,840 --> 00:37:40,580
+الاستجابة للهرمونات تبعته، ومعدّل نموه. فبالتالي هذه
+
+482
+00:37:40,580 --> 00:37:44,200
+كلها بتأثر على نتائج المختبر، فأنا ما أقدر أحكي أنه
+
+483
+00:37:44,200 --> 00:37:49,880
+النتيجة الطبيعية لفحص معين قيمة بحد ذاتها، لأ.
+
+484
+00:37:50,290 --> 00:37:55,030
+النتيجة الطبيعية لفحص هي لازم تكون واقعة في range
+
+485
+00:37:55,030 --> 00:38:00,210
+في مدى من إلى، تمام؟ لإن في فروق فردية. فعشان هيك
+
+486
+00:38:00,210 --> 00:38:03,890
+بنراعي الفروق الفردية، وبنعتبر أنه normal يقع من
+
+487
+00:38:03,890 --> 00:38:10,130
+إلى. أي شخص نتيجته خارج هذا المدى اللي من إلى،
+
+488
+00:38:10,130 --> 00:38:14,730
+بنعتبره abnormal، سواء أقل أو أعلى، هو عنده مشكلة، و
+
+489
+00:38:14,730 --> 00:38:21,350
+يجب أن تعالج. طيب، أنواع المختبرات، التدريبية، في عندي
+
+490
+00:38:21,350 --> 00:38:27,050
+طبعًا حسب موقعها، إذا كانت داخل مستشفى، attached to
+
+491
+00:38:27,050 --> 00:38:30,530
+hospital، متصلة بالمستشفى، فبسميها hospital
+
+492
+00:38:30,530 --> 00:38:37,010
+laboratory، تمام؟ وهذه بتجري فحوصات على المرضى، إذا
+
+493
+00:38:37,010 --> 00:38:41,110
+كان المختبر
+
+494
+00:38:41,110 --> 00:38:47,340
+مش متصل بمستشفى، فبسميه private أو community lab.
+
+495
+00:38:47,340 --> 00:38:52,380
+طبعًا مختبر خاص بيستلم عينات من ال... من الأطباء، كان
+
+496
+00:38:52,380 --> 00:38:56,400
+من الأطباء، بيوجهوهم، يعني الطبيب بيحكي للمريض: روح
+
+497
+00:38:56,400 --> 00:39:00,700
+على المختبر، قول لهم واعمل فحص كذا. المريض نفسه
+
+498
+00:39:00,700 --> 00:39:03,940
+بيروح على المختبر، وممكن يجري فحوصات عشان يطمئن على
+
+499
+00:39:03,940 --> 00:39:10,040
+صحته. في بعض الشركات بتعمل تقييمات لهذه المختبرات
+
+500
+00:39:10,040 --> 00:39:16,000
+الخاصة، بحيث إنها هي تشتغلها، وتقيّم... وتشوف هذه
+
+501
+00:39:16,000 --> 00:39:20,660
+الشركات تقيم شغل المختبرات الخاصة، وعلى أساس هذا
+
+502
+00:39:20,660 --> 00:39:26,560
+المختبر بيرتقي، أو أنه بياخد لفت نظر. بنقيم ال
+
+503
+00:39:26,560 --> 00:39:30,740
+quality تبعته من خلال ال... هذا عشان... عشان برضه
+
+504
+00:39:30,740 --> 00:39:34,860
+كمان أنا أعطي ترخيص، أو أجدد له ترخيص، لازم برضه كمان
+
+505
+00:39:34,860 --> 00:39:40,880
+أنا أختبر شغله. أيضًا بيجري داخل الـ private labs
+
+506
+00:39:40,880 --> 00:39:50,040
+يعني أبحاث طبية، ممكن برضه كمان نشتغلها. إذا في عندي
+
+507
+00:39:50,040 --> 00:39:54,700
+نوعين حسب موقعهم: الـ laboratory، الـ hospital lab، و
+
+508
+00:39:54,700 --> 00:39:57,860
+الـ private lab، اللي هو مختبر المستشفى، ومختبر
+
+509
+00:39:57,860 --> 00:40:03,400
+الخاص. طبعًا في حاجة اسمها تدوير عينات، a lot of
+
+510
+00:40:03,400 --> 00:40:06,680
+samples are sent between different labs for
+
+511
+00:40:06,680 --> 00:40:11,000
+uncommon tests. يعني مثلا إحنا فاتحين مختبر خاص
+
+512
+00:40:11,000 --> 00:40:15,300
+private lab على قدنا، وما عنديش كل الأجهزة متوفرة،
+
+513
+00:40:15,300 --> 00:40:19,820
+إجا مريض، المريض هذا بده فحص مثلا growth hormone،
+
+514
+00:40:19,820 --> 00:40:24,420
+فحص هرمون النمو، وهذا أنا ما أقدر أوفر له، يعني، فأنا
+
+515
+00:40:24,420 --> 00:40:29,680
+ممكن آخذ عينة من المريض، وبعدين أبعت العينة بشكل
+
+516
+00:40:29,680 --> 00:40:33,740
+أنا أتواصل من مختبر لمختبر آخر، أبعت العينة على
+
+517
+00:40:33,740 --> 00:40:39,000
+مختبر آخر، هو يعني عنده أجهزة أكثر تطورًا، وعنده
+
+518
+00:40:39,000 --> 00:40:42,520
+فحوصات أكثر، بيقدم خدمات أكثر، أبعت له إياها، وهو
+
+519
+00:40:42,520 --> 00:40:48,880
+يشتغلها، بحيث أنه في النهاية ثمن الفحص بيتقاسمه
+
+520
+00:40:48,880 --> 00:40:54,870
+المختبرين، طبعًا. فإذا it's more cost effective، أنه
+
+521
+00:40:54,870 --> 00:41:00,390
+يعني ال... بي... بيأثر على سعر الفحص، يعني أنه
+
+522
+00:41:00,390 --> 00:41:03,650
+بيوفر في سعر الفحص. مثلا إن مختبرات مثلا تسحب
+
+523
+00:41:03,650 --> 00:41:07,670
+العينة، ومختبرات بتشتغل الشغل. فبالتالي هم هيك
+
+524
+00:41:07,670 --> 00:41:14,950
+بيتقاسموا العينات والـ money ما بينهم. طيب، أنواع
+
+525
+00:41:14,950 --> 00:41:20,670
+المختبرات، أو أقسام المختبرات تبعًا لتخصيصيتها، يعني
+
+526
+00:41:20,670 --> 00:41:25,130
+داخل المختبر لازم يكون في عندي أقسام، في أقسام
+
+527
+00:41:25,130 --> 00:41:29,330
+مختلفة، طبعًا. إن هم ال... ال... الـ main department،
+
+528
+00:41:29,330 --> 00:41:34,170
+الأقسام الأساسية تلاتة، ولكن بتفرع تحتها أشياء أخرى
+
+529
+00:41:34,170 --> 00:41:37,950
+زي مثلا في قسم لازم يكون داخل المختبر، اسمه clinical
+
+530
+00:41:37,950 --> 00:41:42,900
+pathology، اللي هو الأمراض السريرية. طبعًا هذا بي...
+
+531
+00:41:42,900 --> 00:41:46,720
+بيجري فيه كل فحوصات الـ hematology، كل فحوصات
+
+532
+00:41:46,720 --> 00:41:51,400
+الأنسجة، كل فحوصات الأمراض الخلوية، وحتى الـ routine
+
+533
+00:41:51,400 --> 00:41:56,720
+pathology، يعني، فحوصات الـ urine وال... الـ stool.
+
+534
+00:41:56,720 --> 00:42:02,450
+أيضًا فيه. في قسم الـ microbiology، يعني قسم الـ
+
+535
+00:42:02,450 --> 00:42:05,770
+microbiology، يعني قسم الأحياء الدقيقة، اللي بيكشف
+
+536
+00:42:05,770 --> 00:42:09,250
+عن وجود بكتيريا، الـ bacteriology، الـ virology،
+
+537
+00:42:09,250 --> 00:42:14,210
+الفيروسات، الـ mycology، الفطريات، الفطريات، الـ
+
+538
+00:42:14,210 --> 00:42:18,370
+parasitology، الطفيليات، وحتى بعض فحوصات المناعة، و
+
+539
+00:42:18,370 --> 00:42:23,760
+الأمصال بتكون بتجرى داخل هذا القسم. قسم الكيميا، أو
+
+540
+00:42:23,760 --> 00:42:28,760
+الكيميا الحيوية، أو الكيميا الطبية، بنجري فيه فحوصات
+
+541
+00:42:28,760 --> 00:42:32,160
+الكيميا، يعني وظائف الأعضاء، وظائف الكلى، وظائف
+
+542
+00:42:32,160 --> 00:42:37,100
+الكبد وكذا، وأيضًا فحوصات السكر والدهون، وكل هاي
+
+543
+00:42:37,100 --> 00:42:41,240
+الشغلات بنسميها biochemical tests، تمام؟ وأيضًا
+
+544
+00:42:41,240 --> 00:42:43,900
+فحوصات الهرمونات بتجرى داخل هذا القسم.
+
+545
+00:42:46,480 --> 00:42:53,740
+طبعًا بيتفرّعوا، كل قسم بيتفرع إلى فروع داخل القسم.
+
+546
+00:42:54,720 --> 00:42:58,660
+طبعًا في عندي منشأة اسمها الـ blood bank، بنك الدم.
+
+547
+00:42:58,660 --> 00:43:04,980
+طبعًا هذا هو جسم منفرد بنفسه، separate body يعني، أو
+
+548
+00:43:04,980 --> 00:43:09,340
+منشأة مش منفردة بحد ذاتها، يعني ممكن أنا ألاقي بنك
+
+549
+00:43:09,340 --> 00:43:13,380
+دم مالوش علاقة لا بمختبر خاص ولا بمستشفى، زي بنك
+
+550
+00:43:13,380 --> 00:43:20,660
+الدم العام هذا، طبعًا؟ فهو ممكن يكون متصل أو لا يكون
+
+551
+00:43:20,660 --> 00:43:26,000
+متصل بمستشفى. blood bank، طبعًا؟ وإيش وظيفته؟ وظيفته
+
+552
+00:43:26,000 --> 00:43:32,020
+إن هو بيشتغل فحوصات، سواء كان... يعني بيتأكد إن
+
+553
+00:43:32,020 --> 00:43:36,310
+الدم نظيف، ما فيهوش infectious disease، يعني الدم
+
+554
+00:43:36,310 --> 00:43:39,230
+اللي هي... اللي... اللي إحنا بناخده من المتبرعين، و
+
+555
+00:43:39,230 --> 00:43:43,470
+بدنا نصرفه للمستقبلين. أول حاجة هو يتأكد من نظافته،
+
+556
+00:43:43,470 --> 00:43:46,970
+مش فيه بكتيريا ولا فيروسات، مثلا زي الـ hepatitis،
+
+557
+00:43:46,970 --> 00:43:52,570
+إنها ممكن تقضي على المستقبل. وأيضًا من خلاله بيجري
+
+558
+00:43:52,570 --> 00:43:56,570
+فحوصات على عينات الوحدات الدم اللي بناخد... طبعًا،
+
+559
+00:43:56,570 --> 00:44:01,270
+من المتبرعين، بنعرف فصيلة الدم، الـ blood group، نسبة
+
+560
+00:44:01,270 --> 00:44:05,070
+الدم، أو نسبة الـ hemoglobin داخل الوحدة، وأيضًا مدى
+
+561
+00:44:05,070 --> 00:44:10,990
+تطابق ال... ال... الدم هذا. يعني مثلا أجاني متبرع،
+
+562
+00:44:10,990 --> 00:44:14,610
+أخذت منه وحدة دم، وحدة الدم هذه في ال... في الـ
+
+563
+00:44:14,610 --> 00:44:17,090
+blood bank، أنا ببدأ أتأكد إنها نظيفة، ما فيش فيها
+
+564
+00:44:17,090 --> 00:44:20,690
+فيروسات أو بكتيريا ممكن تسبب أمراض مرضية للمستقبل.
+
+565
+00:44:20,690 --> 00:44:25,870
+ببدأ أتأكد من وحدتها، ونسبة الـ hemoglobin فيها. ولما
+
+566
+00:44:25,870 --> 00:44:31,510
+يجيني مستقبل محتاج وحدة الدم هذه، آخذ منه عينة، و
+
+567
+00:44:31,510 --> 00:44:35,610
+أجري اختبار تطابق، عشان أشوف هذه العينة مطابقة للدم
+
+568
+00:44:35,610 --> 00:44:39,790
+تبعه، وبالتالي لو هو أخدها مش هتظهر عليه أعراض
+
+569
+00:44:39,790 --> 00:44:45,010
+ممكن ت... تودي بحياته، أو لأ، تمام؟ فإذا فحوصات
+
+570
+00:44:45,010 --> 00:44:50,110
+التطابق، وفصائل الدم، وأيضًا الفحوصات للـ
+
+571
+00:44:50,110 --> 00:44:53,290
+infectious disease، اللي بتجرى على وحدات الدم اللي
+
+572
+00:44:53,290 --> 00:44:57,310
+بتتلقاها من المتبرعين، هذه كلها بتجرى داخل الـ blood
+
+573
+00:44:57,310 --> 00:45:02,530
+bank، ونقول إن هو ممكن... ممكن يكون، أو لا يكون
+
+574
+00:45:02,530 --> 00:45:06,010
+متصل بمستشفى. طيب،
+
+575
+00:45:07,940 --> 00:45:12,180
+أنواع المختبر، طبعًا حسب الـ service، حسب الخدمة اللي
+
+576
+00:45:12,180 --> 00:45:17,840
+بيقدموها. إحنا لاحظنا أنه حسب المكان، طبعًا، إذا كان
+
+577
+00:45:17,840 --> 00:45:20,440
+متصل في مستشفى، بسميه hospital lab، إذا ما... إذا
+
+578
+00:45:20,440 --> 00:45:27,910
+ما كان، يعني private، فبسميه private lab. أقسام المختبر
+
+579
+00:45:27,910 --> 00:45:32,410
+وأيضًا أنواعه حسب الخدمة اللي بيقدموها، في حاجة
+
+580
+00:45:32,410 --> 00:45:35,130
+بسميها community business lab، يعني مختبر الرعاية
+
+581
+00:45:35,130 --> 00:45:40,530
+الأولية، بيجري فحوصات بسيطة جدًا، اللي هي يعني ال...
+
+582
+00:45:42,740 --> 00:45:46,840
+فحوصات urine و stool ونسبة الدم، ويعني فحوصات
+
+583
+00:45:46,840 --> 00:45:53,980
+بسيطة جدًا. district hospital lab، يعني هو مثلا زي
+
+584
+00:45:53,980 --> 00:45:58,800
+الفحوصات اللي بتكون موجودة في المدينة، يعني مثلا
+
+585
+00:45:58,800 --> 00:46:02,160
+أنا عندي قرية تابعة لمدينة، هذه القرية فيها
+
+586
+00:46:02,160 --> 00:46:07,380
+مختبر صغير. المختبر الصغير هذا إله district hospital
+
+587
+00:46:07,380 --> 00:46:12,400
+مختبر آخر بعيد عنه، موجود في المدينة، متصل معه، يعني
+
+588
+00:46:12,400 --> 00:46:17,560
+في تواصل ما بينهم، في communication، إجت عينة بتتطلب
+
+589
+00:46:17,560 --> 00:46:21,540
+فحوصات دقيقة ومتطورة، بيرسلوها على الـ district
+
+590
+00:46:21,540 --> 00:46:25,820
+hospital lab علشان يشتغلوها، تمام؟ فإذا الـ
+
+591
+00:46:25,820 --> 00:46:29,160
+district hospital lab، زي الفحص... زي المختبر اللي
+
+592
+00:46:29,160 --> 00:46:33,160
+في المدينة، تابع لمختبر في القرية، تمام؟ الـ regional
+
+593
+00:46:33,160 --> 00:46:39,980
+hospital lab، يعني مثلا زي مختبر اللي مثلا عندي عدة
+
+594
+00:46:39,980 --> 00:46:45,240
+مدن، إلها مختبر مرجعي في العاصمة، تمام؟ يعني أكبر
+
+595
+00:46:45,240 --> 00:46:49,220
+شوية من الـ district hospital lab، وأيضًا أو موجود
+
+596
+00:46:49,220 --> 00:46:53,800
+في إقليم معين، أو في منظومة معينة، فبالتالي بيكون
+
+597
+00:46:53,800 --> 00:46:58,660
+مرجعي له عدة مختبرات أصغر منه. الـ central أو الـ
+
+598
+00:46:58,660 --> 00:47:02,200
+public health lab، زي مختبرات منظمة الصحة العالمية.
+
+599
+00:47:02,200 --> 00:47:07,160
+طبعًا هذا إيش؟ بتشرف على مختبرات مش على قد قرى ومدن،
+
+600
+00:47:07,160 --> 00:47:12,260
+لأ، على قد دول، تمام؟ وبتتابع شغل الـ public health
+
+601
+00:47:12,260 --> 00:47:18,700
+إيش يعني؟ يعني بتتابع مستوى الصحة العامة، ل... يعني
+
+602
+00:47:18,700 --> 00:47:25,320
+شريحة كبيرة جدًا من الناس. طيب، إيش يعني lab
+
+603
+00:47:25,320 --> 00:47:28,260
+informatics؟ الـ lab informatics، طبعًا اللي جيتوا
+
+604
+00:47:28,260 --> 00:47:32,380
+الزمان، كان شغل المختبرات، النتائج، يجيني طلب الفحص
+
+605
+00:47:32,380 --> 00:47:37,020
+على ورقة، أنا أجري الفحص، وأسلم النتائج أيضًا على
+
+606
+00:47:37,020 --> 00:47:40,900
+ورقة أخرى، وأبعتها. فكان في تبادل ورق ما بين
+
+607
+00:47:40,900 --> 00:47:45,380
+الأقسام. هلأ إحنا بنشوف كل شيء صار محوسب، حتى
+
+608
+00:47:45,380 --> 00:47:51,500
+تعليمنا الأيام هذه صار محوسبًا، فبالتالي ال... ال...
+
+609
+00:47:51,970 --> 00:47:56,750
+الـ lab informatics هو عبارة عن منظومة محوسبة، من خلالها
+
+610
+00:47:56,750 --> 00:48:02
+
+667
+00:52:30,930 --> 00:52:38,030
+ووصف العلاج. هكذا نكون قد أنهينا جزئية الـ medical
+
+668
+00:52:38,030 --> 00:52:41,410
+laboratories. طبعاً تعرفنا على ماذا يعني مختبر، وماذا يعني
+
+669
+00:52:41,410 --> 00:52:45,430
+clinical أو medical laboratory وما هي وظيفتنا
+
+670
+00:52:45,430 --> 00:52:49,910
+وأنواع المختبرات مع أقسامها وأنواع الخدمات التي
+
+671
+00:52:49,910 --> 00:52:57,430
+تقدمها داخل المختبرات. سنتحدث  جزئية بسيطة عن الـ ...
+
+672
+00:52:57,430 --> 00:53:04,710
+الـ lab safety طبعاً جزئية بسيطة جداً س...نختصرها...
+
+673
+00:53:04,710 --> 00:53:08,770
+يعني هذه مختصرة، موضوع المحاضرة العملية سيكون
+
+674
+00:53:08,770 --> 00:53:14,810
+بإسهاب أكثر عنها طبعاً، قواعد الـ lab safety rules
+
+675
+00:53:14,810 --> 00:53:19,670
+السلامة والأمان داخل المختبر، طبعاً هيَ أمر مهم جداً، هيَ
+
+676
+00:53:19,670 --> 00:53:26,190
+major aspect أمر مهم جداً في كل مختبر، كل طالب طبعاً
+
+677
+00:53:26,190 --> 00:53:32,530
+في المختبر لابد أن يتبع قواعد السلامة والأمان لكل
+
+678
+00:53:32,530 --> 00:53:37,890
+تجربة يجريها، طبعاً قواعد الـ lab safety rules من قواعد
+
+679
+00:53:37,890 --> 00:53:42,610
+السلامة والأمان، حتى العلامات والإشارات، نحن
+
+680
+00:53:42,610 --> 00:53:50,110
+أحياناً نكتب على ورق ملصق أو رموز على الـ ...
+
+681
+00:53:50,110 --> 00:53:54,650
+ملصقة على الأنابيب، على الـ bottles، الزجاجات
+
+682
+00:53:54,650 --> 00:53:59,130
+مطبوعة على الجدران، على الخزائن،  لنُحذر أن هنا يوجد
+
+683
+00:53:59,130 --> 00:54:03,170
+شيء حارق، هنا يوجد شيء قد يؤذي الشخص، هنا شيء ...
+
+684
+00:54:03,170 --> 00:54:09,350
+فبالتالي لابد لنا أن نعرف هذه الأشياء، وأن
+
+685
+00:54:09,350 --> 00:54:13,410
+نتبعها، سواء كانت قواعد، أو أنني ألتزم
+
+686
+00:54:13,410 --> 00:54:16,790
+بالتعليمات المكتوبة على الخزانة مثلاً، ليس من الضروري
+
+687
+00:54:16,790 --> 00:54:20,350
+أن... أي شيء داخل الخزانة خطير، وأنا لستُ أرتدي
+
+688
+00:54:20,350 --> 00:54:23,270
+gloves، فبالتالي سأتبع هذه الأشياء، لماذا؟  لكي
+
+689
+00:54:23,270 --> 00:54:27,100
+أحمي نفسي، وأحمي زملائي classmates الذين
+
+690
+00:54:27,100 --> 00:54:31,220
+داخل الفصل أو داخل المحاضرة، طبعاً ما هي قواعد الـ lab
+
+691
+00:54:31,220 --> 00:54:37,020
+safety rules بشكل عام؟ أنا داخل المختبر لابد أن ألتزم
+
+692
+00:54:37,020 --> 00:54:41,360
+بأن أرتدي الـ lab coat، أرتدي الـ gloves، و
+
+693
+00:54:41,360 --> 00:54:45,380
+أحياناً بعد الـ ... الـ ... العمل المخبري يحتاج أن أرتدي
+
+694
+00:54:45,380 --> 00:54:50,920
+goggles، نظارات، وأحذية مغلقة، تمام؟ صندل لا، كعب
+
+695
+00:54:50,920 --> 00:54:57,660
+لا، الأشياء التي تحقق الأناقة ليست
+
+696
+00:54:57,660 --> 00:55:01,220
+ضرورية داخل المختبر، أحذية مغلقة close shoes لابد
+
+697
+00:55:01,220 --> 00:55:05,570
+أن تكون موجودة، يعني أنا أريد أناقتي أم سلامتي؟
+
+698
+00:55:05,570 --> 00:55:09,590
+لا، سلامتي أهم من الأناقة، تمام؟ فبالتالي لابد أن
+
+699
+00:55:09,590 --> 00:55:14,310
+الـ shoes تكون مغلقة، والـ gloves أمر ... أمر مهم
+
+700
+00:55:14,310 --> 00:55:18,210
+تمام؟ الـ lab coat مطلوب، الـ goggles  ذكرنا أن بعض
+
+701
+00:55:18,210 --> 00:55:23,100
+الأعمال تحتاجها، يعني إذا كان التفاعل متطايراً أو قد
+
+702
+00:55:23,100 --> 00:55:27,280
+يعطي شراراً أو قد يعطي رشاشاً، فبالتالي أنا لابد أن أرتدي
+
+703
+00:55:27,280 --> 00:55:32,700
+goggles، نظارات، لحماية نفسي، طبعاً الـ issues لابد أن
+
+704
+00:55:32,700 --> 00:55:38,740
+تكون مغلقة، تمام؟ ليس من الضروري أن تكون مفتوحة، والأشياء
+
+705
+00:55:38,740 --> 00:55:43,140
+المريحة والعملية، والأنيقة، والـ ... والـ ... الـ new
+
+706
+00:55:43,140 --> 00:55:47,560
+fashion هذه نتركها، سنحققها إن شاء
+
+707
+00:55:47,560 --> 00:55:50,080
+الله خارج المختبر، وداخل المختبر سلامة أولاً.
+
+708
+00:55:52,070 --> 00:55:58,370
+الخواتم طبعاً don't wear خواتم، ماشي، الـ ... الـ ...
+
+709
+00:55:58,370 --> 00:56:01,770
+الناس الذين اعتادت على ارتداء الـ accessories
+
+710
+00:56:01,770 --> 00:56:06,570
+الإكسسوارات ولا تستطيع الخروج من دونها، ستجد نفسها
+
+711
+00:56:06,570 --> 00:56:12,850
+تواجه صعوبة داخل المختبر، فبالتالي ليس من الضروري أن
+
+712
+00:56:12,850 --> 00:56:16,970
+ترتديها، وأيضاً الملابس الواسعة والفضفاضة، الكم
+
+713
+00:56:16,970 --> 00:56:21,780
+النازل كالفراشة، والـ... والملابس الواسعة، و
+
+714
+00:56:21,780 --> 00:56:26,680
+الشيفونات وهذه الأشياء أيضاً ليست مطلوبة طبعاً
+
+715
+00:56:26,680 --> 00:56:31,920
+ليس من الضروري، يعني نرى هذا الشخص يعمل في
+
+716
+00:56:31,920 --> 00:56:34,960
+هذه المنطقة... هذه تسمى safety cabinets، يعني الذي
+
+717
+00:56:34,960 --> 00:56:38,180
+يدخل إليها لابد أن يكون معقماً، و... وأحياناً تكون ...
+
+718
+00:56:38,180 --> 00:56:40,820
+نعمل بأشياء معدية داخل... في داخل هذه
+
+719
+00:56:40,820 --> 00:56:43,910
+الـ cabinet، تمام؟ فهو... هو يرتدي الـ gloves، يعني كان
+
+720
+00:56:43,910 --> 00:56:47,110
+يتعامل مع الأشياء الموجودة في الداخل،  مسك هاتفه
+
+721
+00:56:47,110 --> 00:56:50,650
+الخلوي وهذا خطأ، وأنا أرتدي الـ gloves،
+
+722
+00:56:50,650 --> 00:56:54,950
+وأنا أعمل في... يعني لمست العينات ولمست الأدوات،
+
+723
+00:56:54,950 --> 00:56:59,010
+حتى المواد الكيميائية، ليس من الضروري أن أعود بنفس الـ... الـ
+
+724
+00:56:59,010 --> 00:57:03,850
+gloves، أو بنفس اليدين، لمس الأقلام والهواتف، و
+
+725
+00:57:03,850 --> 00:57:12,170
+حتى مقابض الأبواب، ليس من الضروري، طبعاً يُفضل أن أخلع الـ
+
+726
+00:57:12,170 --> 00:57:16,170
+gloves وأغسل يدي، وبعدين أذهب لأخذ أغراضي، وإلا
+
+727
+00:57:16,170 --> 00:57:20,950
+سأكون قد نقلت مواد معدية أو مواد سامة،
+
+728
+00:57:20,950 --> 00:57:24,110
+وأضعها على أغراضي لأُنشرها بعد ذلك، بعد
+
+729
+00:57:24,110 --> 00:57:28,350
+ما أخرج من المختبر، أنشرها everywhere، طبعاً سأُعطي زميلاتي
+
+730
+00:57:28,350 --> 00:57:31,390
+شيئاً من تخصصي، وزميلاتي من التخصصات الأخرى،
+
+731
+00:57:31,390 --> 00:57:37,190
+سأُعطيهم شيئاً من المواد... مواد سامة ومُعدية وهكذا
+
+732
+00:57:37,190 --> 00:57:43,230
+يعني أكون كمنارة، أُنشر، هذا خطأ طبعاً،
+
+733
+00:57:43,230 --> 00:57:47,580
+يجب أن نُحضر أنفسنا، الأقلام، طبعاً إذا كنت أكتب بها وهي
+
+734
+00:57:47,580 --> 00:57:51,680
+موجودة في أروقة المختبر ليس من الضروري أن أضعها في جيبي أو
+
+735
+00:57:51,680 --> 00:57:56,020
+أنني، يعني شعرت بأن القلم قد تلوث، يعني
+
+736
+00:57:56,020 --> 00:58:02,450
+لا أُعيد وضعه مع أغراضي أو داخل الحقيبة، لا تترك
+
+737
+00:58:02,450 --> 00:58:07,510
+الـ benz burner شغالاً وأذهب لأُحادِث أو... أَتفسّح أو
+
+738
+00:58:07,510 --> 00:58:12,290
+أ... أذهب لأ... أعمل عملاً آخر، لأنه قد
+
+739
+00:58:12,290 --> 00:58:16,890
+يُؤدي إلى حريق، تمام؟ بمجرد أن أنهي عملي، أُطفئ الـ benz
+
+740
+00:58:16,890 --> 00:58:20,590
+burner، وهو موقد الـ benz، لأضمن ألا يكون
+
+741
+00:58:20,590 --> 00:58:24,830
+هناك حريق، وطبعاً أتأكد أن مفتاح الغاز
+
+742
+00:58:24,830 --> 00:58:28,010
+الرئيسي للغاز مغلق، كي لا يحدث تسرب غاز، لأن هناك حوادث كثيرة وقعت
+
+743
+00:58:28,010 --> 00:58:32,270
+مثل، أن يكون
+
+744
+00:58:32,270 --> 00:58:35,250
+هناك تسرب غاز مع إضاءة كهربائية، فيحدث حريق.
+
+745
+00:58:36,170 --> 00:58:39,070
+طبعاً  النساء اللواتي... الحمد لله على نعمة الإسلام، نحن
+
+746
+00:58:39,070 --> 00:58:46,090
+نغطي رؤوسنا، ولكن النساء غير المحجبات يجب ألا يتركن
+
+747
+00:58:46,090 --> 00:58:52,690
+الشعر منسدلاً، يُفضل ربطه أو رفعه، كي لا يكون
+
+748
+00:58:52,690 --> 00:58:56,530
+مُعرضاً لـ...  يعني مثلاً لا يحترق أو يحدث به contamination
+
+749
+00:58:56,530 --> 00:59:02,910
+فيحدث خطر hazard أو contamination، تمام؟ don't
+
+750
+00:59:02,910 --> 00:59:07,150
+food or drink water in the lab ليس من الضروري أن نأكل أو
+
+751
+00:59:07,150 --> 00:59:12,330
+نشرب داخل المختبر أو بجانب أماكن العمل، لأنه قد
+
+752
+00:59:12,330 --> 00:59:16,230
+تتلوث هذه الأطعمة والمشروبات، وبالتالي نؤذي
+
+753
+00:59:16,230 --> 00:59:20,960
+أنفسنا، protect your safety، وأيضاً ليس من الضروري أن
+
+754
+00:59:20,960 --> 00:59:24,640
+يخطر لي أن أتذوق شيئاً داخل
+
+755
+00:59:24,640 --> 00:59:29,860
+المختبر، protect your hand safety، يجب مثلاً عندما
+
+756
+00:59:29,860 --> 00:59:34,880
+ألمس شيئاً ساخناً، مثلاً الـ flask ساخن، أرتدي قفازات عازلة gloves
+
+757
+00:59:34,880 --> 00:59:41,820
+للحرارة، أو أمسكه بواسطة ملقط، تمام؟ كيف أحافظ على
+
+758
+00:59:41,820 --> 00:59:46,820
+الـ... الـ... يدي، سلامة يدي، أنني لا
+
+759
+00:59:46,820 --> 00:59:51,140
+أمسك الأشياء الـ... الزجاج المكسور أو الأدوات
+
+760
+00:59:51,140 --> 00:59:54,940
+الحادة بيدي مباشرةً، لا، لابد أن أتعامل معها
+
+761
+00:59:54,940 --> 01:00:00,510
+بحذر، وأيضاً أحرص على غسل يدي باستمرار، هذا أيضاً
+
+762
+01:00:00,510 --> 01:00:05,370
+يُحافظ على سلامة اليدين، الـ electrical safety، هو
+
+763
+01:00:05,370 --> 01:00:10,710
+بمجرد أن أنهي عملي بجهاز computer، أفصل قابسه من
+
+764
+01:00:10,710 --> 01:00:19,430
+الكهرباء، وطبعاً ممنوع أن ألمس أو أترك الأسلاك والأحبال
+
+765
+01:00:19,430 --> 01:00:24,690
+الكهربائية مكشوفة، طبعاً الـ chemical safety ليس من الضروري
+
+766
+01:00:24,690 --> 01:00:33,030
+أن ألمس أو أشعر أو أشم أو أتذوق أي مادة كيميائية داخل
+
+767
+01:00:33,030 --> 01:00:39,500
+المختبر، تمام؟ وأيضاً ليس من الضروري أن أخلط مواد كيميائية هكذا
+
+768
+01:00:39,500 --> 01:00:43,200
+يخطر لي حسّ... الحسّ الكيميائي عندي، وأبدأ أخلط
+
+769
+01:00:43,200 --> 01:00:47,020
+مواد كيميائية، طالما أنا لم أُعطِكم تعليمات
+
+770
+01:00:47,020 --> 01:00:52,280
+بأن أضيف مادة ألف إلى باء، أنتِ لا تُضيفين،
+
+771
+01:00:52,280 --> 01:00:55,550
+تمام؟ لأن خلط المواد الكيميائية مع بعضها البعض قد
+
+772
+01:00:55,550 --> 01:00:59,430
+تكون نتائجه... أو عواقبه وخيمة، keep lids on
+
+773
+01:00:59,430 --> 01:01:04,830
+chemical container، طبعاً المواد... آسفة، الأغطية يجب أن
+
+774
+01:01:04,830 --> 01:01:08,130
+أترك الغطاء عليها، keep lids، الـ lids يعني
+
+775
+01:01:08,130 --> 01:01:11,090
+الغطاء، يجب أن أضع... أضعها على الـ chemical
+
+776
+01:01:11,090 --> 01:01:14,290
+container، لأنه في النهاية هناك مواد قد تمتص
+
+777
+01:01:14,290 --> 01:01:19,590
+الرطوبة، فيتغير تركيزها، هناك مواد قد تمتص
+
+778
+01:01:19,590 --> 01:01:25,410
+الرطوبة، فتصبح مُشتعلة، هناك مواد قد تتبخر، فبالتالي
+
+779
+01:01:25,410 --> 01:01:28,530
+يجب أن أبقي الأغطية lids على الـ
+
+780
+01:01:28,530 --> 01:01:33,390
+container، يعني أُغلقها، don't take any culture
+
+781
+01:01:33,390 --> 01:01:38,350
+out of the lab، إذا كنت أعمل بمزارع، تمام؟ ليس من الضروري
+
+782
+01:01:38,350 --> 01:01:43,570
+هذه أطباق عبارة عن مزارع، بها مواد مُغذية، وتنمو
+
+783
+01:01:43,570 --> 01:01:46,930
+عليها البكتيريا أو الفطريات، ومن خلالها أعرف
+
+784
+01:01:46,930 --> 01:01:52,410
+على الفطريات أو البكتيريا التي قد تسبب عدوى، تمام؟
+
+785
+01:01:52,410 --> 01:01:57,810
+ف... وهذه أيضاً عبارة عن culture، ولكن هنا مثلاً
+
+786
+01:01:57,810 --> 01:02:01,590
+المادة الغذائية صلبة، هنا المادة الغذائية سائلة، طبعاً
+
+787
+01:02:01,590 --> 01:02:07,330
+هذه
+
+788
+01:02:07,330 --> 01:02:10,810
+الـ cultures، هذه المواد المُغذية التي تنمو عليها
+
+789
+01:02:10,810 --> 01:02:16,270
+البكتيريا، ممنوع أن آخذها خارج المختبر، طبعاً؟ لماذا؟
+
+790
+01:02:16,270 --> 01:02:21,050
+لأنها قد تنتشر وتنتقل، يعني في النهاية تنتشر
+
+791
+01:02:21,050 --> 01:02:27,060
+العدوى خارج المختبر، لا، فبالتالي يجب، سواء كانت الـ
+
+792
+01:02:27,060 --> 01:02:31,840
+الـ... الـ... نتيجة الـ... يعني نتيجة المزرعة هذه
+
+793
+01:02:31,840 --> 01:02:35,780
+البكتيريا التي نمت لديّ، معدية أو غير معدية، تسبب
+
+794
+01:02:35,780 --> 01:02:39,360
+أمراضاً أو لا تسبب أمراضاً، تُعامل جميعها على أنها
+
+795
+01:02:39,360 --> 01:02:41,960
+pathogenic، ماذا يعني أن أُعامل... أُعاملها على أنها
+
+796
+01:02:41,960 --> 01:02:46,120
+pathogenic؟ على أنها مُمرضة، تمام؟ يعني ممنوع أن
+
+797
+01:02:46,120 --> 01:02:49,620
+أتأكد أن هذه البكتيريا لا تسبب أمراضاً،  خلاص... خلاص
+
+798
+01:02:49,620 --> 01:02:53,820
+أ... أشتغل بها، لا، don't trust  لا تثقي بالبكتيريا، لا تُفَقِّي في
+
+799
+01:02:53,820 --> 01:02:57,500
+البكتيريا، لأنها قد تتحول فجأة من non
+
+800
+01:02:57,500 --> 01:03:02,900
+-pathogenic إلى pathogenic، ويعني وهناك العديد من البكتيريا
+
+801
+01:03:02,900 --> 01:03:06,460
+نسميها البكتيريا الانتهازية، التي تنتَهاز الفرصة وهي
+
+802
+01:03:06,460 --> 01:03:12,690
+ليست ممرضة، ولكن تنتَهِز فرصة وتصبح ممرضة، أيضاً الـ
+
+803
+01:03:12,690 --> 01:03:17,650
+liquid culture، وهي الـ culture السائلة، هذه يجب
+
+804
+01:03:17,650 --> 01:03:21,090
+أن تكون داخل الـ rack، هذا الـ rack هو
+
+805
+01:03:21,090 --> 01:03:24,770
+حاملها، وتكون موجودة على الـ bench إلى الداخل
+
+806
+01:03:24,770 --> 01:03:28,270
+كي لا تُسكب أو تُكسر أو تنتشر مني، تنتشر
+
+807
+01:03:28,270 --> 01:03:32,930
+العدوى أو تنتشر البكتيريا على... على الأرض، و
+
+808
+01:03:32,930 --> 01:03:37,190
+أيضاً ليس من الضروري، يعني المهم ألا تُكسر وتتسرب
+
+809
+01:03:38,290 --> 01:03:43,690
+عندي، don't engage in practical jokes or horseplay
+
+810
+01:03:43,690 --> 01:03:47,830
+in the lab، يعني مثلاً لا تحاولوا أن تنصبوا لبعضكم
+
+811
+01:03:47,830 --> 01:03:54,310
+فخاً داخل المختبر، أو أن تُظهروا حسّ الدعابة لديكم، وتبدأوا تعملون مع بعضكم مجازفة،
+
+812
+01:03:54,310 --> 01:03:59,930
+تضعين لصديقتك مادة غريبة، ولا تُخليها تشمها أو أنكِ مثلاً
+
+813
+01:03:59,930 --> 01:04:06,510
+ترشينها عليها، أو تخوّفينها، تجلبين لها شيئاً غريباً داخل
+
+814
+01:04:06,510 --> 01:04:12,350
+المختبر، لأن أيّ ركضة، تركض مثلاً وهي لا شعورياً
+
+815
+01:04:12,350 --> 01:04:18,050
+خائفة أو تُكسر أو ينسكب عليها شيء، قد إذا
+
+816
+01:04:18,050 --> 01:04:23,370
+قدّمْتِ لها مادة أخرى، يعني يلحقها إذا أو
+
+817
+01:04:23,370 --> 01:04:28,630
+إذا مثلاً طلع... يعني قلتِ لها إنها ليست سامة...
+
+818
+01:04:28,630 --> 01:04:33,070
+لمستها، قالت شمّي، جرّبته وهي لمستها ولم تقل... يعني أنتِ
+
+819
+01:04:37,570 --> 01:04:42,150
+تتركيها، في عام من الأعوام
+
+820
+01:04:42,150 --> 01:04:47,170
+حدث... كنا نعمل فحصاً... في مختبر الكيمياء
+
+821
+01:04:47,170 --> 01:04:53,310
+الحياتية، وكان... كنا نستخدم الـ acetic acid المركز
+
+822
+01:04:53,310 --> 01:04:59,450
+طبعاً، خالٍ يعني مركز جداً، فال... البنت قالت لزميلتها
+
+823
+01:04:59,450 --> 01:05:07,450
+شمّيه، يعني زميلتها شمّته، يعني بقيت فترة طويلة، يعني
+
+824
+01:05:07,450 --> 01:05:12,170
+تقريباً نصف ساعة اختناق لديها، يعني بكت البنت بشدة
+
+825
+01:05:12,170 --> 01:05:15,790
+جداً، وليست قادرة على الشمّ، وهو أصلاً عندما شمّته
+
+826
+01:05:15,790 --> 01:05:23,210
+أحدث تهيجاً للأنسجة في الداخل، يعني نحن البنت...
+
+827
+01:05:23,270 --> 01:05:28,790
+احتجنا أن نُخليها تشرب ماءً وأنها... نضعها
+
+828
+01:05:28,790 --> 01:05:33,330
+في مكان به تهوية جيدة، ونُفكّ لها الشال، ويعني
+
+829
+01:05:33,330 --> 01:05:38,390
+قمنا بإسعافات أولية حتى نُخلصها من أثر أنها هي
+
+830
+01:05:38,390 --> 01:05:43,370
+شمّت حمضاً قوياً، فبالتالي رجاءً لا تمزحوا مع بعضكم هكذا
+
+831
+01:05:43,370 --> 01:05:46,550
+مزاح جديد، حسّ الدعابة خارج المختبر، ليس
+
+889
+01:09:57,490 --> 01:10:00,890
+الحمض هذا مثلاً لو أنا بدي أضيف عليه ماء ممكن يشتعل
+
+890
+01:10:00,890 --> 01:10:04,290
+لأ، بروح بحط له base عشان يعادله، يعمله
+
+891
+01:10:04,290 --> 01:10:08,950
+neutralization، بعدين ب... ب... أنشفه وبامسح بماء
+
+892
+01:10:08,950 --> 01:10:12,410
+بعدين بامسح بمعقم، وبالتالي هيك يعني في خطوات
+
+893
+01:10:12,410 --> 01:10:19,350
+للتخلص من آثار الحوادث، وبالتالي لازم نسمع... نسأل
+
+894
+01:10:19,350 --> 01:10:23,270
+المسؤولة عنها، علشان هي تحكيلنا كيف... كيف نتعامل
+
+895
+01:10:23,270 --> 01:10:27,330
+معها، ونقلل من أثر الضرر اللي ممكن يلحق فينا أو
+
+896
+01:10:27,330 --> 01:10:32,210
+في زملائنا نتيجة هذا الحادث، كسر أو... انسكاب أو أي
+
+897
+01:10:32,210 --> 01:10:39,020
+شيء، إيش الأدوات اللي لازم نستخدمها لحتى نحقق الـ
+
+898
+01:10:39,020 --> 01:10:42,900
+safety، الأمان؟ طبعاً في عندي شاور، الشاور هذا و
+
+899
+01:10:42,900 --> 01:10:49,080
+مجرد ما أني أنا أشدّ الجزء هذا، طبعاً هذا الجزء،
+
+900
+01:10:49,080 --> 01:10:52,440
+مجرد ما أشده بتنزل ماء، هذا طبعاً إذا إيش انكبّ
+
+901
+01:10:52,440 --> 01:10:56,520
+علينا، أي... على ال... على إيدينا أو على أي جزء من
+
+902
+01:10:56,520 --> 01:11:01,510
+جسمنا، مادة، ممكن تكون حارقة أو ممكن تكون مؤذية. الـ
+
+903
+01:11:01,510 --> 01:11:07,130
+...في عندي شاور... شاور للـ eye، طبعاً كمان هذا إذا
+
+904
+01:11:07,130 --> 01:11:11,710
+كان أي رذاذ داخل... داخل العين، لازم أنه نعرض العين
+
+905
+01:11:11,710 --> 01:11:17,550
+لهذا الشاور. الـ fire blanket، إذا احنا مثلاً شخص
+
+906
+01:11:17,550 --> 01:11:22,690
+يعني لا سمح الله، انكبّ عليه شيء مشتعل أو عم بينحرق
+
+907
+01:11:22,690 --> 01:11:28,650
+فلازم نلفّه في بطانية عشان تمنع من أنّه هذا النار
+
+908
+01:11:28,650 --> 01:11:33,510
+تبقى مشتعلة، وفي عندي fire extinguisher برضه كمان
+
+909
+01:11:33,510 --> 01:11:42,970
+اللي هو الجرة الغاز اللي بتطفي النيران، طبعاً
+
+910
+01:11:42,970 --> 01:11:51,450
+في عندي إشارات للـ hazards، تمام؟ في عندي إشارات
+
+911
+01:11:51,450 --> 01:11:58,640
+للخطر، علامات الخطر، إيش يعني هذه الإشارات؟ مثلاً إذا
+
+912
+01:11:58,640 --> 01:12:02,560
+أنا شفت... ممكن أشوفها على أدوات أو على الخزانات
+
+913
+01:12:02,560 --> 01:12:07,620
+أو على الحائط، موجودة طبعاً، يعني إذا أنا شفت هذه
+
+914
+01:12:07,620 --> 01:12:10,840
+العلامة، معناها المكان هذا فيه animal، والـ animal
+
+915
+01:12:10,840 --> 01:12:14,160
+هذه ممكن تكون معديّة أو ممرضة أو في... يعني احنا
+
+916
+01:12:14,160 --> 01:12:20,000
+بنُجري عليها تجارب، فمش لازم أُقترب من هذا المكان هذا
+
+917
+01:12:20,000 --> 01:12:22,960
+يعني أن الأدوات اللي... العلامة اللي بتكون موجودة
+
+918
+01:12:22,960 --> 01:12:28,420
+على هذه الأدوات أو ال... الأماكن، يعني الأدوات هذه
+
+919
+01:12:28,420 --> 01:12:33,520
+أو الأماكن موجود داخلها أدوات sharp، يعني أدوات
+
+920
+01:12:33,520 --> 01:12:41,080
+حادة، ممكن أنها تسبب جروح أو جروح عميقة مثلاً
+
+921
+01:12:41,080 --> 01:12:45,700
+للشخص إذا ما تعامل معها بطريقة صحيحة. heat hazard،
+
+922
+01:12:45,700 --> 01:12:51,160
+الأدوات الساخنة أو الأجهزة اللي بتعطي حرارة، لازم
+
+923
+01:12:51,160 --> 01:12:53,860
+يكون عليها هذه العلامة، عشان أنا أول ما أشوفها، أن
+
+924
+01:12:53,860 --> 01:12:56,760
+أنا ما ألامسهاش بإيدي على طول مباشرة، ثانياً أعرف أن هي
+
+925
+01:12:56,760 --> 01:13:02,370
+ممكن تعمل حرق، فبالتالي الأشياء الساخنة لازم أنا
+
+926
+01:13:02,370 --> 01:13:06,150
+ألبس الـ gloves السميكة قبل ألامسها أو أمسكها بالـ
+
+927
+01:13:06,150 --> 01:13:10,090
+الملقاط. مثل ما حكيت هنا، glassware hazard، يعني هذا
+
+928
+01:13:10,090 --> 01:13:14,330
+المكان فيه أشياء زجاجية، أنا مش لازم مثلاً الـ rough
+
+929
+01:13:14,330 --> 01:13:17,930
+handling، هذا موجود عليه هذه العلامة، يعني أدوات
+
+930
+01:13:17,930 --> 01:13:21,990
+زجاجية، مش لازم أنا أجي وأنا مش متدرب على الـ rough handling أجي
+
+931
+01:13:21,990 --> 01:13:28,190
+أحاول أن أنا أخذ شيء منه، ممكن يجعل أدوات زجاجية
+
+932
+01:13:28,190 --> 01:13:33,130
+تنكسر وتؤذيني. chemical hazard، طبعاً في مواد كيميائية
+
+933
+01:13:33,130 --> 01:13:39,790
+بتكون موجودة، ممكن مؤذية، حارقة، أو بتسبب تهيج للجلد
+
+934
+01:13:39,790 --> 01:13:46,990
+أو ممكن أن اجتماعها بيبقى مؤذٍ، أو فبالتالي بتسبب
+
+935
+01:13:46,990 --> 01:13:50,870
+خطر معين، أو مش لازم أخلطها مع بعضها البعض، فبالتالي
+
+936
+01:13:50,870 --> 01:13:53,610
+أنا بتعامل معها بحذر، بيكون عليها هذه العلامة
+
+937
+01:13:53,610 --> 01:13:56,800
+chemical hazard، هذه المواد أنا مش لازم لا أشمها ولا
+
+938
+01:13:56,800 --> 01:14:01,080
+ألامسها أو أتذوقها أو حتى أخلطها مع مواد أخرى بدون
+
+939
+01:14:01,080 --> 01:14:04,020
+تعليمات، لازم أستنى، ألمس، أيش بتحكي لي كيف أتعامل مع
+
+940
+01:14:04,020 --> 01:14:07,440
+هذه المادة، عشان أنا أظلّ سالم. electrical hazard،
+
+941
+01:14:07,440 --> 01:14:10,480
+طبعاً هذه العلامة اللي يعني على الأسلاك اللي بشوفها
+
+942
+01:14:10,480 --> 01:14:14,460
+عند الأجهزة الكهربائية، أنّه هذا الجهاز من الخطر أنّه
+
+943
+01:14:14,460 --> 01:14:19,650
+يظلّ موصول بالكهرباء، فلازم أفصله بعد ما أخلص. eye and face
+
+944
+01:14:19,650 --> 01:14:24,010
+...hazard، طبعاً أن المكان هذا بيتنبعث
+
+945
+01:14:24,010 --> 01:14:29,110
+منه رذاذ أو أبخرة ممكن تكون مؤذية للعين أو الوجه،
+
+946
+01:14:29,110 --> 01:14:33,490
+فبالتالي لازم أن مثلاً أحطّ shield، أحطّ mask على
+
+947
+01:14:33,490 --> 01:14:38,650
+وجهي أو شيء، فبالتالي بدّي انتبه لهذه العلامة.
+
+948
+01:14:38,650 --> 01:14:41,830
+العلامة هذه بتكون موجودة على الأشياء القابلة
+
+949
+01:14:41,830 --> 01:14:46,110
+للاشتعال أو اللي ممكن تحترق. العلامة هذه بتكون
+
+950
+01:14:46,110 --> 01:14:49,990
+موجودة على الـ biohazard، هتشوفوها على السلات اللي
+
+951
+01:14:49,990 --> 01:14:54,590
+بنحطّ فيها الأدوات اللي لازم تتعقم قبل نُطلفها.
+
+952
+01:14:54,590 --> 01:14:58,490
+حكيت لكم إنّه في أدوات لازم أنا أعقمها قبل أُطلفها،
+
+953
+01:14:58,490 --> 01:15:03,850
+فهذه الـ biohazard موجودة عليها، يعني خطر بيولوجي،
+
+954
+01:15:03,850 --> 01:15:08,210
+إيش خطر بيولوجي؟ يعني فيروسات، بكتيريا، فطريات، أشياء
+
+955
+01:15:08,210 --> 01:15:12,600
+معديّة ممكن تكون موجودة. هتشوفوا... هنشوفها على السلات و
+
+956
+01:15:12,600 --> 01:15:18,140
+على ال... الـ safety boxes وبعض الأدوات وبعض
+
+957
+01:15:18,140 --> 01:15:22,540
+الأكياس، هرجيكم إياها، خليكم تاخدوا انتباهكم منها.
+
+958
+01:15:22,540 --> 01:15:27,100
+laser radiation، طبعاً يعني مثلاً هذه يعني أن المواد
+
+959
+01:15:27,100 --> 01:15:31,700
+مشعّة، وأنا مش لازم أقترب أو ألامسها، هيك ليش؟ لأنّه
+
+960
+01:15:31,700 --> 01:15:37,510
+أنا إذا بدّي أقترب من هاي ال... المادة، يعني أنا أخذت
+
+961
+01:15:37,510 --> 01:15:40,870
+إشعاع بإيدي، روحت لمست كتبي، الكتب صارت مشعّة،
+
+962
+01:15:40,870 --> 01:15:44,090
+لمست زميلتي، زميلتي صار في عندها إشعاع،كذا، و
+
+963
+01:15:44,090 --> 01:15:48,750
+الإشعاعات carcinogenic، مسببة للـ cancer، فبالتالي
+
+964
+01:15:48,750 --> 01:15:55,630
+مشكلة، أو... وكمان ممكن تعمل abnormal hormonal
+
+965
+01:15:55,630 --> 01:15:59,170
+profile، إيش يعني؟ يعني بتخرب كل شغل ال... الـ
+
+966
+01:15:59,170 --> 01:16:04,050
+hormones داخل الجسم، فبالتالي لازم أنا انتبه لهي
+
+967
+01:16:04,050 --> 01:16:08,070
+المواد المشعّة، حكينا أن نُلفّها حسب التعليمات، في طرق
+
+968
+01:16:08,070 --> 01:16:13,770
+معيّنة. الأداة هذه يعني برضه كمان أن في عندي إشعاع
+
+969
+01:16:13,770 --> 01:16:17,950
+هذا إشعاع laser، وهو هذي إشعاع من أنواع أخرى أيضاً
+
+970
+01:16:17,950 --> 01:16:22,430
+برضه كمان، يعني مواد مشعّة وخطرة. explosive، يعني هذه
+
+971
+01:16:22,430 --> 01:16:25,590
+المادة قابلة للانفجار، مش لازم أنّه أنا مثلاً أتركها
+
+972
+01:16:25,590 --> 01:16:29,490
+مفتوحة، مش لازم أ... أ... أحطّها في... أ... أضلّ أرجّ
+
+973
+01:16:29,490 --> 01:16:33,090
+فيها، مش لازم أ... أ... أضيف عليها مواد تساعد من
+
+974
+01:16:33,090 --> 01:16:37,230
+انفجارها، فبالتالي بدّي أتعامل مع كل المواد هذه
+
+975
+01:16:37,230 --> 01:16:41,430
+أتعامل طبقا للتعليمات، كيف يعني طبقا للتعليمات؟
+
+976
+01:16:41,430 --> 01:16:44,470
+يعني الـ master بتحكيلكم أضيفوا بالأول كذا، بعدين
+
+977
+01:16:44,470 --> 01:16:49,050
+كذا، طبعاً أنا ليش حكيت لكم أضيفوا بالأول ال... المادة
+
+978
+01:16:49,050 --> 01:16:53,160
+الفلانية، عشان لو أنتم عكسْتوا ممكن يصير في مشكلة، طبعاً
+
+979
+01:16:53,160 --> 01:16:56,840
+فبالتالي بتنتبهوا لتعليمات، مُستوى منيح، واحنا
+
+980
+01:16:56,840 --> 01:17:01,620
+طبعاً يعني الشيء ما بيناش ياكم تخافوا كثير، لأ احنا
+
+981
+01:17:01,620 --> 01:17:05,880
+بنكون منتبهين كثير معاكم ومركّزين، وبنمشي خطوة
+
+982
+01:17:05,880 --> 01:17:09,020
+بخطوة، وبالعكس أنتم بتكونوا يعني موجودين على الـ
+
+983
+01:17:09,020 --> 01:17:14,180
+bench حواليّ، أنا بكون أول شيء بشتغل التجربة ب...
+
+984
+01:17:14,180 --> 01:17:18,800
+بإيدي، بورجيكم كيف التجربة بتنشغل، بتشوفوها لأول مرة
+
+985
+01:17:18,800 --> 01:17:24,010
+بأيديكم... بعيونكم، sorry، وبعد ما تشوفوا ال...
+
+986
+01:17:24,010 --> 01:17:31,110
+الشيء، بتروحوا تشتغلوا بأيديكم، بعدين طبعاً وأنتم
+
+987
+01:17:31,110 --> 01:17:34,890
+بتشتغلوا أنا بكون متابعة معاكم كمان، يعني ما بَسيبكم
+
+988
+01:17:34,890 --> 01:17:37,790
+تشتغلوا هيك لحالكم، لأ، يعني أنتم بتكونوا شفتوا
+
+989
+01:17:37,790 --> 01:17:41,250
+التجربة لأول مرة، وأنا بشتغلها بعد ما أكون أنا
+
+990
+01:17:41,250 --> 01:17:45,930
+شرحتها، يعني بتكون التجربة مرت عليكي للمرة الثانية، و
+
+991
+01:17:45,930 --> 01:17:49,410
+بعدين لما تيجي تشتغليها بأيديكي، أنا بمشي معاكم
+
+992
+01:17:49,410 --> 01:17:52,810
+خطوة بخطوة، بقول لكم يلا خطوة رقم واحد، أضيفي المادة
+
+993
+01:17:52,810 --> 01:17:56,010
+كذا، خطوة رقم اثنين، أضيفي المادة كذا، وطبعاً
+
+994
+01:17:56,010 --> 01:17:59,490
+ما بننقلش للخطوة رقم اثنين إلا لما يكون الكل أضاف
+
+995
+01:17:59,490 --> 01:18:02,710
+والكل اشتغل، والكل ماشي، علشان في النهاية نكون
+
+996
+01:18:02,710 --> 01:18:07,590
+كلنا ماشيين زي بعض، وكلنا بنشتغل مع بعض، الكل أخذ
+
+997
+01:18:07,590 --> 01:18:10,910
+...منتبه، وأخذ التعليمات أول بأول، لكن لو تركت
+
+998
+01:18:10,910 --> 01:18:14,410
+مثلاً ناس خلصت وناس لسه، وناس سابت من نص الشغل،
+
+999
+01:18:14,410 --> 01:18:19,050
+حيكون في فوضى، حيكون في ناس مش سامعة تعليمات كويس،
+
+1000
+01:18:19,050 --> 01:18:22,730
+في ناس ملتزمة كويس، فلأ، خطوة بخطوة ومع بعض، و
+
+1001
+01:18:22,730 --> 01:18:27,230
+متابعين أول بأول، علشان في النهاية تكونوا بتشتغلوا
+
+1002
+01:18:27,230 --> 01:18:31,730
+صح، وبتتعلموا صح، ومركزين معايا لكل كلمة بحكيها،
+
+1003
+01:18:31,730 --> 01:18:38,450
+كل كلمة بحكيها بتكون مهمة. في عندي أشياء أخرى من الـ
+
+1004
+01:18:38,450 --> 01:18:47,090
+...الرموز، هي inflammable، يعني مادة قابلة للاشتعال،
+
+1005
+01:18:47,090 --> 01:18:52,150
+تمام، هذه مادة بتسبب تهيج للجسم، مادة حارقة بتسبب
+
+1006
+01:18:52,150 --> 01:18:55,610
+حروق. الـ biohazard عرفنا إيش يعني، الـ explosion
+
+1007
+01:18:55,610 --> 01:19:00,870
+قابلة للانفجار، عرفنا إيش يعني. هذه مادة سامة،
+
+1008
+01:19:00,870 --> 01:19:06,580
+هذه مادة قابلة للأكسدة والاشتعال. وهذه طبعاً المادة
+
+1009
+01:19:06,580 --> 01:19:10,640
+هذه، إذا أنا لازم أتلفها بطريقة معيّنة، مش لازم أوصي
+
+1010
+01:19:10,640 --> 01:19:15,280
+...يعني أكبها كبّ بالحوض أو بالسلة، لأنها لو وصلت
+
+1011
+01:19:15,280 --> 01:19:19,500
+للطرب أو للنباتات أو للبحار، لل... ممكن تؤذي
+
+1012
+01:19:19,500 --> 01:19:23,200
+النباتات أو تؤذي ثروة السمكية أو تؤذي الحيوانات،
+
+1013
+01:19:23,200 --> 01:19:28,600
+فبالتالي بتسبب خطر على البيئة، تعمل تلوث بيئي، فإذا
+
+1014
+01:19:28,600 --> 01:19:32,000
+وجدت هذه المادة... العلامة على أي مادة فأنا لازم
+
+1015
+01:19:32,000 --> 01:19:36,380
+أتلفها بطريقة صحيحة. وهذه معناها مادة مشعّة وحكينا
+
+1016
+01:19:36,380 --> 01:19:41,580
+إيش يعني، هيك بنكون خلصنا مختصر الـ lab safety، و
+
+1017
+01:19:41,580 --> 01:19:44,620
+عرفنا إيش يعني الـ medical laboratory، هذا عنوان
+
+1018
+01:19:44,620 --> 01:19:52,250
+محاضرتنا. ال... المادة... النظرية كان، وراح إن شاء
+
+1019
+01:19:52,250 --> 01:19:57,170
+الله نحكي عن الـ lab safety أيضاً في العمل، بمعلومات
+
+1020
+01:19:57,170 --> 01:20:01,730
+أخرى، تمام؟ هتشوفوا فيها تكرار، تمام؟ ولكن هنضيف بعض
+
+1021
+01:20:01,730 --> 01:20:05,050
+المعلومات الجديدة، وإن شاء الله تكون بسيطة وسهلة
+
+1022
+01:20:05,050 --> 01:20:08,150
+عليكم، يعطيكم ألف عافية، وشكراً لكم.
diff --git a/PL9fwy3NUQKwY9jl8xw-bWkVZkWcGGyspd/QS6fztFTb_Y_postprocess.srt b/PL9fwy3NUQKwY9jl8xw-bWkVZkWcGGyspd/QS6fztFTb_Y_postprocess.srt
new file mode 100644
index 0000000000000000000000000000000000000000..12c2a336a3f6a9f408e2d0b4701bd7b567102743
--- /dev/null
+++ b/PL9fwy3NUQKwY9jl8xw-bWkVZkWcGGyspd/QS6fztFTb_Y_postprocess.srt
@@ -0,0 +1,4088 @@
+1
+00:00:05,720 --> 00:00:09,280
+السلام عليكم ورحمة الله وبركاته اليوم ان شاء الله
+
+2
+00:00:09,280 --> 00:00:14,000
+رح ناخد أول محاضرة نظرية في موضوع المقدمة في
+
+3
+00:00:14,000 --> 00:00:18,300
+التحليل الطبية أو مقدمة في المختبرات الطبية ان شاء
+
+4
+00:00:18,300 --> 00:00:21,380
+الله اليوم موضوعنا عن ال medical laboratories رح
+
+5
+00:00:21,380 --> 00:00:25,560
+نعرف إيش يعني مختبر طبي، إيش رسالة المختبر الطبي
+
+6
+00:00:25,560 --> 00:00:30,240
+أو الهدف الذي يجب أن يحققه المختبر الطبي، وإيش
+
+7
+00:00:30,240 --> 00:00:33,520
+طبيعة الأعمال اللي بيقوم فيها وأنواع المختبرات
+
+8
+00:00:33,520 --> 00:00:38,350
+الطبية بإذن اللهولكن في البداية يجب أن نعرج على
+
+9
+00:00:38,350 --> 00:00:44,630
+مصطلح اسمه health care provider مزودو الصحة أو
+
+10
+00:00:44,630 --> 00:00:49,310
+العناية الصحية إيش يعني مزودو العناية الصحية؟ هذا
+
+11
+00:00:49,310 --> 00:00:54,530
+المصطلح بيندرج تحت .. تحته any individual أي شخص،
+
+12
+00:00:54,530 --> 00:01:00,750
+أي مؤسسة، أي وكالة، أجنسي هي من شأنها أنها تزودأو
+
+13
+00:01:00,750 --> 00:01:05,910
+تقدم health care أو health services لشخص
+
+14
+00:01:05,910 --> 00:01:09,310
+المستفيدين من الخدمات الصحية يعني health care
+
+15
+00:01:09,310 --> 00:01:14,310
+provider مزودية الرعاية الصحية هو مصطلح بيشمل أي
+
+16
+00:01:14,310 --> 00:01:19,510
+شخص، أي مؤسسة، أي وكالة بتقدم رعاية صحية أو خدمات
+
+17
+00:01:19,510 --> 00:01:24,010
+صحية للناس اللي يعتبروا مستهلكين لهذه الخدمات
+
+18
+00:01:24,010 --> 00:01:32,280
+الصحية اللي هم بتعريف آخر المرضىوبيندرج تحت هاي
+
+19
+00:01:32,280 --> 00:01:36,500
+الأشخاص ممكن يكونوا ال physicians، الأطباء، ال
+
+20
+00:01:36,500 --> 00:01:42,020
+dentist، أطباء الأسنان، الممرضين، الأم الأسز اللي
+
+21
+00:01:42,020 --> 00:01:46,740
+هم احنا ال medical laboratory scientist تمام
+
+22
+00:01:46,740 --> 00:01:50,580
+medical laboratory scientist اللي هم احنا تمام ال
+
+23
+00:01:50,580 --> 00:01:56,890
+mental health counselor اللي هم يعنيالمزودي أو
+
+24
+00:01:56,890 --> 00:02:00,750
+يعني الاستشاريين حول الصحة النفسية والصحة العقلية
+
+25
+00:02:00,750 --> 00:02:04,910
+ال nursing التمريد وخدمات أخرى يعني زي التصوير
+
+26
+00:02:04,910 --> 00:02:11,650
+التدبي و الحكمة و التفارعات الأخرى لخدمات الصحية
+
+27
+00:02:11,650 --> 00:02:15,470
+كلهم هدول بيندرجوا تحت مصطلح health care provider
+
+28
+00:02:16,090 --> 00:02:20,550
+في عصر التكنولوجيا الحديثة تمام صار محت .. صرنا
+
+29
+00:02:20,550 --> 00:02:23,550
+محتاجين لحتى نزود او نعمل health care .. health
+
+30
+00:02:23,550 --> 00:02:29,070
+care delivery نزود او نقدم خدمات صحية لازم ان يكون
+
+31
+00:02:29,070 --> 00:02:32,970
+في عندي كتير او so many different personnel و
+
+32
+00:02:32,970 --> 00:02:36,530
+specialists ايش يعني الكلام هذا؟ زمان كان بس في
+
+33
+00:02:36,530 --> 00:02:41,120
+طبيب و في حكيم و خلاص يعنيما .. ماكنش فيه لا
+
+34
+00:02:41,120 --> 00:02:46,480
+صيدلاني ولا كان في مختبر ولا تبع تصوير طب دي، هاي
+
+35
+00:02:46,480 --> 00:02:51,200
+التخصصات كلها نشأت جديد مع تقدم التكنولوجيا، صرنا
+
+36
+00:02:51,200 --> 00:02:55,200
+محتاجين many personnel، يعني أشخاص كتير، موظفين
+
+37
+00:02:55,200 --> 00:03:00,360
+كتير، بتخصصات مختلفة، حتى كمان صرنا محتاجين التخص
+
+38
+00:03:00,360 --> 00:03:03,520
+.. في ال .. في المؤسسات اللي بتقدم رعاية صحية،
+
+39
+00:03:03,520 --> 00:03:06,720
+صرنا محتاجين تخصصات ما قالها علاقة، يعني ال .. ال
+
+40
+00:03:06,720 --> 00:03:10,590
+.. ال medicine مش هو ال background تبعهازي مثلا ال
+
+41
+00:03:10,590 --> 00:03:13,870
+Secretariat التدبية و الإدارة الصحية و الإدارة
+
+42
+00:03:13,870 --> 00:03:17,150
+التدبية، هاي كلها كانت .. يعني اللي هم علاقة
+
+43
+00:03:17,150 --> 00:03:22,250
+بأرشفة الملفات و حفظ الملفات و استخراجها و نماذج
+
+44
+00:03:22,250 --> 00:03:28,450
+ال .. الحفظ نماذج التشخيص و نماذج طلب الفحوصات،
+
+45
+00:03:28,450 --> 00:03:32,290
+هاي .. هاي عملهم تمام؟ ولكن برضه كمان زرنا
+
+46
+00:03:32,290 --> 00:03:38,050
+محتاجينهم في ال .. منظومة التقديم الرعاية الصحية
+
+47
+00:03:38,820 --> 00:03:42,560
+إذن مع تقدم التكنولوجيا صيرنا محتاجين so many
+
+48
+00:03:42,560 --> 00:03:45,320
+different personnel and specialists صيرنا بنعرف
+
+49
+00:03:45,320 --> 00:03:50,140
+إيش يعني هاي الجملة تمام ال caregivers الناس اللي
+
+50
+00:03:50,140 --> 00:03:55,600
+بتعطيني رعاية صحية إيش لازم يتوفر فيها شروط أول
+
+51
+00:03:55,600 --> 00:03:58,880
+حاجة لازم يكون عندها understanding and working
+
+52
+00:03:58,880 --> 00:04:02,740
+knowledge يعني عندها معرفة عندها background خلفية
+
+53
+00:04:02,740 --> 00:04:09,510
+بإمكانها إنها هياش تعمل تقدم diagnostic evaluation
+
+54
+00:04:09,510 --> 00:04:14,790
+يعني تقيم وضع المريض وتقييم تشخيصي لحتى تقدم رعاية
+
+55
+00:04:14,790 --> 00:04:18,850
+صحية صح تمام؟ ومن ضمن هدول الناس اللي هم ال
+
+56
+00:04:18,850 --> 00:04:21,530
+caregivers او ال care provider health care
+
+57
+00:04:21,530 --> 00:04:24,680
+providerاللي هم احنا حكينا ال medical laboratory
+
+58
+00:04:24,680 --> 00:04:29,640
+scientist فبالتالي ال laboratory و ال diagnostic
+
+59
+00:04:29,640 --> 00:04:33,460
+test تبعته المختبر و الفحوصات التشخيصية اللي بتقام
+
+60
+00:04:33,460 --> 00:04:38,140
+او بتجري داخل المختبر هي عبارة عن أداة مهمة جدا لل
+
+61
+00:04:38,140 --> 00:04:44,060
+health care delivery لتقديم الرعاية الصحيةولو انها
+
+62
+00:04:44,060 --> 00:04:46,540
+.. انها هي هذه الخدمات اللي احنا بنقدمها not
+
+63
+00:04:46,540 --> 00:04:49,460
+therapeutic، اش يعني not therapeutic؟ يعني أنا بس
+
+64
+00:04:49,460 --> 00:04:52,740
+.. بس أجرف حصات طبية، انا فعليا ما بكون ساهمت في
+
+65
+00:04:52,740 --> 00:04:56,660
+علاج المريض بشكل direct ولكن انا بساهم في علاج
+
+66
+00:04:56,660 --> 00:05:01,070
+المريض indirect، كيف هذا الاشي بيصير؟من خلال أنه
+
+67
+00:05:01,070 --> 00:05:05,350
+مثلا المريض الطبيب بيكون عنده ال .. ال history،
+
+68
+00:05:05,350 --> 00:05:09,910
+التاريخ أو ال .. ال .. الصورة العامة عن ال ..
+
+69
+00:05:09,910 --> 00:05:12,930
+التاريخ ال .. ال .. ال patient، هل عنده أمراض
+
+70
+00:05:12,930 --> 00:05:16,650
+ورثية، هل هو شكا من أمراض أو من أعراض سابقة، يعني
+
+71
+00:05:16,650 --> 00:05:20,630
+صورة .. صورة المريض أو ال .. ال .. الملف ال .. ال
+
+72
+00:05:20,630 --> 00:05:25,960
+history تبعه، تمام؟ السيرة تبعته، المرضيةو بيكون
+
+73
+00:05:25,960 --> 00:05:29,040
+أيضا كمان عند الطبيب physical examination اش يعني
+
+74
+00:05:29,040 --> 00:05:33,600
+physical examination يعني عملله .. عملله تشخيص شاف
+
+75
+00:05:33,600 --> 00:05:39,540
+الأعراض و ال .. ال .. علامات اللي ظاهرة على المريض
+
+76
+00:05:39,540 --> 00:05:44,100
+عملله فحص و شاف اش وضع المريض من الشكل الخارجي
+
+77
+00:05:44,100 --> 00:05:51,240
+تمام؟ و احنا كMLS بنقدم خدمات صحينا therapeutic بس
+
+78
+00:05:51,240 --> 00:05:55,790
+يعني بت .. بت ..كد بتساعد أنه الطبيب يتأكد to
+
+79
+00:05:55,790 --> 00:06:00,650
+confirm diagnosis تمام و من خلال أن أنا أقدم value
+
+80
+00:06:00,650 --> 00:06:03,730
+بال information معلومات قيمة عن وضع المريض
+
+81
+00:06:03,730 --> 00:06:09,210
+فبالتالي الطبيب بيقدر أنه يشخص صح فبيوصف علاج صح و
+
+82
+00:06:09,210 --> 00:06:13,610
+بالتالي بيصير في عنده response لل therapy المريض
+
+83
+00:06:13,610 --> 00:06:18,770
+راح تتحسن حالته ال patient status راح تتحسن و أيضا
+
+84
+00:06:18,770 --> 00:06:23,950
+راح يصير في عنده response لل therapyال .. نلخص إيش
+
+85
+00:06:23,950 --> 00:06:26,930
+اللي .. اللي حكيناها، حكينا إنه في .. مع
+
+86
+00:06:26,930 --> 00:06:31,010
+التكنولوجيا الحديثة صيرنا محتاجين تخصصات أكثر، عدد
+
+87
+00:06:31,010 --> 00:06:35,150
+موظفين أكثر، كان زمان المنشأات الصحية بيقتصر عملها
+
+88
+00:06:35,150 --> 00:06:39,310
+على فقط ممرض وطبيب، الآن أنتوا المنشأة الصحية طبعا
+
+89
+00:06:39,310 --> 00:06:42,690
+عندكم مثلا ميثال مستشفى الشفاء، عدد مهول من
+
+90
+00:06:42,690 --> 00:06:48,670
+الموظفينsecretaria، إدارة صحية، إدارة طبية، أرشفة
+
+91
+00:06:48,670 --> 00:06:56,670
+ملفات، حتى موظفين للإشراف على الجودة وضمان لصحة
+
+92
+00:06:56,670 --> 00:07:01,530
+العمل وجودة العمل، عندك أطباء، أخصائي مختبرات
+
+93
+00:07:01,530 --> 00:07:06,250
+طبية، صيادلة، إذن عدد كبير من ال personnel و ال
+
+94
+00:07:06,250 --> 00:07:11,670
+specialists التخصصات أيضاوحكينا إنه ال .. الشخص
+
+95
+00:07:11,670 --> 00:07:14,530
+اللي بيقدم الرعاية الصحية لازم يكون في عنده
+
+96
+00:07:14,530 --> 00:07:17,410
+understanding .. understanding and working
+
+97
+00:07:17,410 --> 00:07:21,410
+knowledge يعني لازم يكون في عنده خلفية ويفهم للوضع
+
+98
+00:07:21,410 --> 00:07:28,710
+الصحي لل ..المريض او الحالة الطبية اللى بتيجي
+
+99
+00:07:28,710 --> 00:07:32,410
+لعنده علشان يساعد في حاجة اسمها diagnostic
+
+100
+00:07:32,410 --> 00:07:36,290
+evaluation اللى هو يعني التقييم التشخيصى الصحى
+
+101
+00:07:36,290 --> 00:07:41,930
+وحكينا ايضا ان المختبرات بغض النظر عن انه هي ما
+
+102
+00:07:41,930 --> 00:07:46,310
+بتقدم therapeutic approach يعني ما بتعطيش بتساهمش
+
+103
+00:07:46,310 --> 00:07:51,030
+مباشرة في العلاج ولكن بتساهم بطريقة غير مباشرة
+
+104
+00:07:51,030 --> 00:07:56,100
+للعلاجكيف بتساهم بطريقة غير مباشرة في العلاج بإنها
+
+105
+00:07:56,100 --> 00:08:00,960
+تعملي diagnostic test بتساهق بتساعد الطبيب على إنه
+
+106
+00:08:00,960 --> 00:08:05,380
+يشخق يأكد ال diagnosis تبعه من خلال كيف يعني أكد
+
+107
+00:08:05,380 --> 00:08:09,700
+التشخيص تبعه من خلال رابط هذا النتائج الفحوصات
+
+108
+00:08:09,700 --> 00:08:14,360
+تبعتي مع ال history تبع المريض الملف المريض و
+
+109
+00:08:14,360 --> 00:08:18,940
+السيرة المرضية تبعتهمع الأعراض والعلامات الظاهرة
+
+110
+00:08:18,940 --> 00:08:22,040
+على المريض اللي ظهرت من خلال الفحص ال physically
+
+111
+00:08:22,040 --> 00:08:26,000
+وبالتالي بيصير في عندنا معلومات قيمة عن وضع المريض
+
+112
+00:08:26,000 --> 00:08:32,440
+ومدى استجابته للعلاج إذا فحوصات أجريت بدقة نتائج
+
+113
+00:08:32,440 --> 00:08:37,700
+صحيحة تشخيص صحيح وبالتالي علاج فعال هذه هي السلسلة
+
+114
+00:08:37,700 --> 00:08:42,000
+اللي بيقوم بتمر فيها مرحلة ال health care
+
+115
+00:08:42,000 --> 00:08:47,450
+providingتمام؟ و أيضا احنا كمختبرات مش بس بنساعد
+
+116
+00:08:47,450 --> 00:08:51,750
+ال .. ال .. الاطباء على انهم هم يعملوا confirm
+
+117
+00:08:51,750 --> 00:08:57,110
+diagnosis لأ احنا ايضا بنساعد في ال epidemiology
+
+118
+00:08:57,110 --> 00:09:01,930
+اش يعني ال epidemiology؟ يعني مدى التقصي لمدى
+
+119
+00:09:01,930 --> 00:09:06,130
+انتشار الوباء فعليا انتوا شايفين مثلا حاليا احنا
+
+120
+00:09:06,130 --> 00:09:11,980
+في .. في ظل جائحة جائحة coronavirusمين اللي اللي
+
+121
+00:09:11,980 --> 00:09:15,960
+على قدم و ساق و من خلالهم بنعرف عدد العينات اللي
+
+122
+00:09:15,960 --> 00:09:20,120
+تنفحصها و ال positive result كام عددهم و ال
+
+123
+00:09:20,120 --> 00:09:24,340
+negative result و مين هذا كله من خلال ال lab work
+
+124
+00:09:24,340 --> 00:09:30,080
+بنعرفه فبالتالي احنا بنساهم من خلال المساعدة او
+
+125
+00:09:30,080 --> 00:09:37,210
+التقصي المتعلق بالوبائيات وايضابال research
+
+126
+00:09:37,210 --> 00:09:42,510
+purposes أيضا المختبر إله دور في أغراض البحث
+
+127
+00:09:42,510 --> 00:09:46,250
+العلمى كيف يعني أغراض البحث العلمى يعني من خلال
+
+128
+00:09:46,250 --> 00:09:51,450
+الدراسات و الأبحاث تجري داخل المختبرات لما نحكي
+
+129
+00:09:51,450 --> 00:09:57,170
+مثلا والله تم اكتشاف أنه دواء أو formula معين
+
+130
+00:09:57,170 --> 00:10:04,150
+كصيغة معينة أو مادة كيميائية معينة مثلا إلها دور
+
+131
+00:10:04,150 --> 00:10:08,420
+فعال في إذابة الجلطاتتمام؟ هاي كيف احنا اكتشفناها؟
+
+132
+00:10:08,420 --> 00:10:12,680
+اكتشفناها من خلال ان احنا أجرينا عدة تحوصات او عدة
+
+133
+00:10:12,680 --> 00:10:18,000
+تجارب داخل المختبر و أكدنا هاي العلاقة فبالتالي
+
+134
+00:10:18,000 --> 00:10:22,600
+أيضا الأبحاث المحترمة والنتائج والاكتشافات العظيمة
+
+135
+00:10:22,600 --> 00:10:28,580
+تخرج من المختبر المختبر بيأكدال .. الفحوصات بيساعد
+
+136
+00:10:28,580 --> 00:10:32,420
+في تقصي الحالات الوبائية وظهور ال .. و تكتشاف ظهور
+
+137
+00:10:32,420 --> 00:10:35,940
+الأمراض الجديدة، بيساعد في إجراء الدراسات العلمية
+
+138
+00:10:35,940 --> 00:10:40,600
+و التقدم العلمي، المختبر أو أخصائي المختبر هو عين
+
+139
+00:10:40,600 --> 00:10:46,020
+الطبيب الثانيةالطبيب له عين تان عين بتشوف ال
+
+140
+00:10:46,020 --> 00:10:49,180
+physical examination اللي هي العلامات الظاهرة
+
+141
+00:10:49,180 --> 00:10:55,000
+خارجيا على المرض و طبعا هذه علامات طبعا متداخلة في
+
+142
+00:10:55,000 --> 00:10:58,020
+كل .. في معظم الأمراض كإيش يعني متداخلة في معظم
+
+143
+00:10:58,020 --> 00:11:01,700
+الأمراض؟ يعني مثلا الأعراض اللي ظاهرة على مريض
+
+144
+00:11:01,700 --> 00:11:10,610
+معين هي عبارة عن حمة ألم في العضلات والعظاميعني
+
+145
+00:11:10,610 --> 00:11:18,090
+مثلا ممكن تكون diarrhea فهذه العلامات التلاتة كتير
+
+146
+00:11:18,090 --> 00:11:22,550
+أمراض بيكون أعراضهم وجود قيق وإسهال وقلم في
+
+147
+00:11:22,550 --> 00:11:27,730
+العضلات والمفاصل أو في العظام وأيضا ارتفاع في درجة
+
+148
+00:11:27,730 --> 00:11:32,030
+الحرارة ال influenza والرشح والاتهابات أو النزلات
+
+149
+00:11:32,030 --> 00:11:37,740
+المعاوية وكتير أمراض حتى COVID-19 منهذه العلامات
+
+150
+00:11:37,740 --> 00:11:41,840
+من أعراضه، إذا المريض مش راح .. الطبيب راح .. مش
+
+151
+00:11:41,840 --> 00:11:48,980
+راح يقدر يحصر ال .. ال .. الأمراض اللي ممكن يخمنها
+
+152
+00:11:48,980 --> 00:11:54,300
+و تفسرله أو تشخصله إيش المريض عندهالا اذا احنا
+
+153
+00:11:54,300 --> 00:11:58,820
+عملنا له فحوصات وحكينا له والله انه المريض هذا
+
+154
+00:11:58,820 --> 00:12:04,560
+اللي عنده حمة وقلام في العضلات والعظام وعنده ايضا
+
+155
+00:12:04,560 --> 00:12:12,100
+دياريا مثلا هو عنده مثلا طفل معين في القمعة وجود
+
+156
+00:12:12,100 --> 00:12:15,180
+..كشفنا عن وجود طفل معين في القمعة، اذا راح يروح
+
+157
+00:12:15,180 --> 00:12:19,990
+فكرة المريضفكرة الطبيب أنه هذا المريض بعاني من
+
+158
+00:12:19,990 --> 00:12:25,150
+نازلة معاوية مش من virus ولا influenza ولا رشح ولا
+
+159
+00:12:25,150 --> 00:12:29,850
+أي إشي تاني، إذا الطبيب بعينه شاف العلامات
+
+160
+00:12:29,850 --> 00:12:34,690
+الخارجية اللي ظاهرة على المريض وإحنا بالعين
+
+161
+00:12:34,690 --> 00:12:40,810
+الثانية للطبيب شفنا العلامات الداخليةاستكشفنا داخل
+
+162
+00:12:40,810 --> 00:12:44,970
+المريض إيش فيه من خلال إن أحنا فحصنا عيناته و
+
+163
+00:12:44,970 --> 00:12:49,170
+أكدنا ال diagnosis، إذا إحنا دورنا لا يقل أهمية عن
+
+164
+00:12:49,170 --> 00:12:54,310
+الطبيب دي التشخيص، تمام؟ إحنا ساعد و ساند و عين
+
+165
+00:12:54,310 --> 00:12:58,600
+الطبيب الثانيطيب introduction to medical
+
+166
+00:12:58,600 --> 00:13:01,340
+laboratory technology ايش يعني مقدمة في التحاليل
+
+167
+00:13:01,340 --> 00:13:07,360
+الطبية؟ هو عبارة حكينا هو basic course مثاق تأسيسي
+
+168
+00:13:07,360 --> 00:13:11,420
+بيساعد الطلاب على انهم ياخدوا ال essential
+
+169
+00:13:11,420 --> 00:13:15,780
+knowledge المعرفة الأساسية و المهارات الأساسية
+
+170
+00:13:15,780 --> 00:13:20,400
+لحتى يعرفوا كيف يتصرفوا داخل المختبرات الطبية و
+
+171
+00:13:20,400 --> 00:13:25,440
+هذا الكلام طبعا احنا حكيناه في محاضرة التقديمطبعا
+
+172
+00:13:25,440 --> 00:13:30,280
+ايش اهمية المختبرات الطبية او اهمية المصاد طبعا
+
+173
+00:13:30,280 --> 00:13:37,400
+هيعلمنا اهمية او مدى اهمية الخدمات اللي بيقدمها
+
+174
+00:13:37,400 --> 00:13:42,310
+المختبر ايضا دورال medical laboratory technologist
+
+175
+00:13:42,310 --> 00:13:45,030
+انت ك medical laboratory technologist او medical
+
+176
+00:13:45,030 --> 00:13:49,010
+laboratory scientist ايش دورك بالضبط برضه كمان رح
+
+177
+00:13:49,010 --> 00:13:53,370
+تتعرفي عليه من خلال هذا المثاق كيف انا استخدم ال
+
+178
+00:13:53,370 --> 00:13:56,530
+laboratory wares ادوات المختبر ال instruments
+
+179
+00:13:56,530 --> 00:14:00,250
+اجهزة المختبر و كيف انا اقوم بعملية ال
+
+180
+00:14:00,250 --> 00:14:03,700
+sterilization technique اللي هي كيف اناأقوم
+
+181
+00:14:03,700 --> 00:14:09,180
+بعمليات التعقيم لحتى أضمن حكينا سلامتي، سلامة
+
+182
+00:14:09,180 --> 00:14:12,500
+المريض الذي يرتدي المختبر وسلامة زملائي اللي
+
+183
+00:14:12,500 --> 00:14:16,360
+بيشتغلوا معايا في نفس المكان، في نفس بيئة العمل و
+
+184
+00:14:16,360 --> 00:14:22,370
+لحتى أعمل sorry لحتى أعمل preventionand control of
+
+185
+00:14:22,370 --> 00:14:27,010
+laboratory accident طبعا بدرايتي كيف أستخدم
+
+186
+00:14:27,010 --> 00:14:30,350
+الأدوات كيف أستخدم الأجهزة كيف أعقم كيف أستخدم
+
+187
+00:14:30,350 --> 00:14:34,870
+المواد الكيميائية هيك أنا بكون يعني اتعلمت أو قطعت
+
+188
+00:14:34,870 --> 00:14:40,690
+شغط كتير كبير في كيف أمنع و أتحكم بحدوث ال .. ال
+
+189
+00:14:40,690 --> 00:14:44,090
+.. ال accidents اللي هي الحوادث المختبرة اللي ممكن
+
+190
+00:14:44,090 --> 00:14:49,610
+تكون ضارة أو ممكن تكون تقضيني أو تقضي زملائيأيضا
+
+191
+00:14:49,610 --> 00:14:53,250
+institution of quality control system كيف أنا أطبق
+
+192
+00:14:53,250 --> 00:14:59,370
+معايير الجودة و ضمان السلامة و ضمان الجودة داخل
+
+193
+00:14:59,370 --> 00:15:04,350
+المختبر لحتى أرفع مستوى ال accuracy و ال precision
+
+194
+00:15:04,630 --> 00:15:08,090
+عشان انا أكون بحصل على .. ب .. بشتغل مختار ..
+
+195
+00:15:08,090 --> 00:15:14,210
+مفحوصات و بحصل على نتائج بمستوى عالي من الدقة و
+
+196
+00:15:14,210 --> 00:15:17,690
+الصحة أيضا، هذا طبعا بضمنلي ال equality، برفع
+
+197
+00:15:17,690 --> 00:15:20,670
+مستوى ال quality طب .. طبعا انا .. طبعا اذا اشتغلت
+
+198
+00:15:20,670 --> 00:15:24,310
+بدقة عالية و صحة عالية و جودة عالية إيش بستفيد؟
+
+199
+00:15:24,310 --> 00:15:29,690
+بستفيد انه انا ب .. بكسب ثقةال consumers او ال
+
+200
+00:15:29,690 --> 00:15:33,750
+customers ثقة للمرضى اللى بيرتادوا المختبر تسمعوا
+
+201
+00:15:33,750 --> 00:15:37,290
+ان هذا المختبر نتائجه موثوقه هذا المختبر نتائجه مش
+
+202
+00:15:37,290 --> 00:15:42,610
+كتير ليش لأنه اذا .. اذا طبقت معايير ل quality
+
+203
+00:15:42,610 --> 00:15:46,890
+جيدا داخل المختبرات اذا انا ب .. بحصل على نتائج
+
+204
+00:15:46,890 --> 00:15:52,030
+accurate precise معايير جودة عاليةإذا أنا برتقي
+
+205
+00:15:52,030 --> 00:15:55,570
+بالمختبر تبعي وبخدمات المختبر تبعتي وبالتالي بكسب
+
+206
+00:15:55,570 --> 00:15:59,550
+ثقة ال .. ال .. الأشخاص اللي بيرتعدوا المختبر أو
+
+207
+00:15:59,550 --> 00:16:02,790
+بيستفيدوا من خدمات المختبر وبالتالي هيك أنا بكون
+
+208
+00:16:02,790 --> 00:16:08,550
+بحص العائد كتير منيح و أيضا بتزيد ال confidence
+
+209
+00:16:08,550 --> 00:16:13,870
+تبعتي بتزيد ثقتي بنفسي طالما أنه أنا مطبقة لمعايير
+
+210
+00:16:13,870 --> 00:16:18,770
+الجودة أو يعني ماشي على قوانين الجودة العالمية
+
+211
+00:16:20,600 --> 00:16:24,080
+اش يعني تعريف مختبر laboratory؟ laboratory هو
+
+212
+00:16:24,080 --> 00:16:27,640
+عبارة عن facility لما أحكي عن facility يعني منشأة
+
+213
+00:16:27,640 --> 00:16:32,800
+تمام؟ المختبر هو عبارة عن منشأة that provides
+
+214
+00:16:32,800 --> 00:16:39,540
+controlled condition تزودنا بظروف محكمة و مضبوطة
+
+215
+00:16:39,540 --> 00:16:45,440
+تمام؟ ليش؟من خلالها انه انا بقدر اشتغل scientific
+
+216
+00:16:45,440 --> 00:16:49,320
+and technological research من خلال هذه الظروف
+
+217
+00:16:49,320 --> 00:16:56,240
+المحكمة و المضبوطة انا بقدر اشتغل ابحاث علمية بقدر
+
+218
+00:16:56,240 --> 00:17:01,280
+اجري تجارب experiments and measurements و قياسات
+
+219
+00:17:01,280 --> 00:17:07,240
+تمام ايش يعني ظروف مضبطة او مضبوطة يعني controlled
+
+220
+00:17:07,240 --> 00:17:12,630
+conditionو الله انا لازم افح .. لازم اخزن ال
+
+221
+00:17:12,630 --> 00:17:16,970
+reagents تبعتي و المواد الكيميائية على درجة حرارة
+
+222
+00:17:16,970 --> 00:17:23,890
+مثلا من سفر لاربعة، اذا لازم انا اتأكد انه كل يوم
+
+223
+00:17:23,890 --> 00:17:28,590
+اجي اشيك على درجة حرارة التلاجة انها هي ده يعني
+
+224
+00:17:28,590 --> 00:17:33,520
+درجة حرارتها مضبوطةتمام؟ أنا لازم عشان أنمي
+
+225
+00:17:33,520 --> 00:17:37,480
+بكتيريا و أتعرف عليها و أشخص ال .. نوع البكتيريا
+
+226
+00:17:37,480 --> 00:17:42,580
+اللي عاملة مرض لمريد معين لازم أنمي هذه البكتيريا
+
+227
+00:17:42,580 --> 00:17:47,140
+على درجة حرارة 37 إذا جهاز ال incubator الحاضنة
+
+228
+00:17:47,140 --> 00:17:50,120
+اللي أنا بدي أنمي فيه البكتيريا هذه كل يوم بدي
+
+229
+00:17:50,120 --> 00:17:54,870
+أشيك عليه و أتأكد أنه درجة حرارته 37إذا أنا بدي
+
+230
+00:17:54,870 --> 00:17:59,850
+أشتغل بدي أفصل عينات مثلا بدي أفصلها على جهاز
+
+231
+00:17:59,850 --> 00:18:07,650
+الجهاز هذا طبعا إله عدد لفات معينة في الدقيقة
+
+232
+00:18:07,650 --> 00:18:11,830
+الواحدة بيشتغل على الكهربا إذا لازم أشيك على طيار
+
+233
+00:18:11,830 --> 00:18:16,920
+الكهربا اللي بيجي لعنده شغله لازم يكون فيهالجهاز
+
+234
+00:18:16,920 --> 00:18:22,120
+هذا يكون موزون احقق ال balance تبعه اذا لما انا
+
+235
+00:18:22,120 --> 00:18:25,900
+اكون متأكدة ان كل الأجهزة و كل الأدوات تبعتي
+
+236
+00:18:25,900 --> 00:18:31,100
+مضبوطة اذا انا وفرت اش controlled condition ضروف
+
+237
+00:18:31,100 --> 00:18:35,360
+مضبوطة لحتى اشتغل scientific research تجارب
+
+238
+00:18:35,360 --> 00:18:39,880
+experiments و قياسات measurements و تكون كلها
+
+239
+00:18:39,880 --> 00:18:46,340
+اشمالهانتائجها تعتمد تمام؟ لكن مثلا لما تكوني
+
+240
+00:18:46,340 --> 00:18:49,940
+التلاجة مرة بتفصل مرة بترتفع طب انا مش متأكدة ان
+
+241
+00:18:49,940 --> 00:18:53,120
+ال reagent هذا او المادة الكيميائية اللي موجودة في
+
+242
+00:18:53,120 --> 00:18:57,500
+ضايلة فعالة ولا خربت درجة الحرارة مش ثابتة معناه
+
+243
+00:18:57,500 --> 00:19:01,440
+انا مش هتأكد الفحص تبعي او التجربة تبعتي اللي بدي
+
+244
+00:19:01,440 --> 00:19:04,760
+اشتغلها باستخدام المادة الكيميائية هذه هتعطيني
+
+245
+00:19:04,760 --> 00:19:09,360
+نتائج دقيقة فبالتاليهذا معنى كلمة controlled
+
+246
+00:19:09,360 --> 00:19:13,240
+condition كل شيء داخل المختبر يجب أن يكون مضبوط
+
+247
+00:19:13,240 --> 00:19:19,480
+نشكي عليه نتأكد من .. من أن هو بيشتغل في الظروف
+
+248
+00:19:19,480 --> 00:19:23,980
+اللي لازم يكون موجود عليها طبعا medical laboratory
+
+249
+00:19:23,980 --> 00:19:28,390
+طبعا laboratory highعبارة عن كلمة أو مصطلح عام
+
+250
+00:19:28,390 --> 00:19:32,210
+medical laboratory هو مصطلح خاص و نفس المعنى
+
+251
+00:19:32,210 --> 00:19:37,310
+clinical laboratory مختبر طبي او مختبر سريري هو
+
+252
+00:19:37,310 --> 00:19:41,330
+عبارة عن المختبر او المنشأة تمام المختبر او
+
+253
+00:19:41,330 --> 00:19:46,330
+المنشأة اللي من خلالها انا اش بشتغل اش نوعية
+
+254
+00:19:46,330 --> 00:19:49,770
+التجارب اللي بدي اشتغلها بدي اشتغلها على clinical
+
+255
+00:19:49,770 --> 00:19:54,630
+specimen تمام؟ومن خلالها انا بدي اخد نتائج get
+
+256
+00:19:54,630 --> 00:19:58,810
+information علشان تساعدني في ال diagnosis ومن ثم ل
+
+257
+00:19:58,810 --> 00:20:02,410
+treatment و ايضا ل prevention of disease امنع
+
+258
+00:20:02,410 --> 00:20:05,170
+انتشار الأمراض من خلال ان انا احقق ال diagnosis
+
+259
+00:20:05,170 --> 00:20:09,470
+صحية treatment صحية فبالتالي بحد من انتشار الأمراض
+
+260
+00:20:09,470 --> 00:20:13,580
+بهذا الشكلطيب إيش الفرق ما بين laboratory و
+
+261
+00:20:13,580 --> 00:20:16,020
+medical laboratory؟ حكينا laboratory هي الكلمة
+
+262
+00:20:16,020 --> 00:20:20,840
+العامة أو التعريف العام لمصطلح مختبر، طبعا انتوا
+
+263
+00:20:20,840 --> 00:20:28,600
+يمكن ارتدتوا او شوفتوا مبنى لمختبرات كان مكون من
+
+264
+00:20:28,600 --> 00:20:32,240
+رد التوابق، الفيزيا عندهم مختبراتالكيميا عندهم
+
+265
+00:20:32,240 --> 00:20:35,960
+مختبرات، احنا عندنا في العلوم الطبية المخبرية
+
+266
+00:20:35,960 --> 00:20:40,340
+عندنا مختبرات وحتى في علوم الأرض والبيئة عندهم
+
+267
+00:20:40,340 --> 00:20:44,160
+مختبرات ولكن طبعا كلهم نفس الاشي عندهم control
+
+268
+00:20:44,160 --> 00:20:47,360
+condition وبيشتغلوا research وبيشتغلوا experiments
+
+269
+00:20:47,360 --> 00:20:52,140
+و measurementsولكن نوعية الدراسات أو نوعية ال
+
+270
+00:20:52,140 --> 00:20:54,800
+measurements و الفحوصات و ال experiment و التجارب
+
+271
+00:20:54,800 --> 00:20:59,380
+اللي بيشتغلوها تختلف تمام؟ مثلا بالكيميا بيجيبوا
+
+272
+00:20:59,380 --> 00:21:02,260
+مادة unknown مش معروفة بيجروا عليها فحوصات
+
+273
+00:21:02,260 --> 00:21:04,940
+كيميائية معينة بيتعرفوا على مثلا ال molecular
+
+274
+00:21:04,940 --> 00:21:10,020
+weight تبعتها بيتعرفوا على خصائصها هي Redox ولا ..
+
+275
+00:21:11,550 --> 00:21:15,410
+مثلا Reductant أو Oxidant بتعرفوا عليها أنها ..
+
+276
+00:21:15,410 --> 00:21:18,810
+يعني بالنهاية بياخدوا صورة كاملة عن المادة و ممكن
+
+277
+00:21:18,810 --> 00:21:21,390
+يعرفوها أنها هذه المادة أو هذا المحلول أو هذه
+
+278
+00:21:21,390 --> 00:21:28,030
+الصيغة .. صيغتها الكيميائية تصير معروفةال .. الأرض
+
+279
+00:21:28,030 --> 00:21:33,070
+والبيئة بيجروا في خوصات و أبحاث و قياسات ولكن ممكن
+
+280
+00:21:33,070 --> 00:21:38,190
+تكون على عينات تربة عينات ماء عينات سخور يعني هيك
+
+281
+00:21:38,190 --> 00:21:41,950
+.. هيك علم الأرض والبيئة لكن احنا ال medical
+
+282
+00:21:41,950 --> 00:21:47,170
+laboratory بتخصصنا بنجري في خوصاتtests بنشتري
+
+283
+00:21:47,170 --> 00:21:51,310
+افحوصات على إيش؟ على clinical specimen إيش يعني
+
+284
+00:21:51,310 --> 00:21:56,070
+clinical specimen عينة مرضية أو سريرية يعني عينة
+
+285
+00:21:56,070 --> 00:22:00,530
+أنا أخدتها من شخص هو suspected شك فيه أنه يكون
+
+286
+00:22:00,530 --> 00:22:04,310
+مريض طبعا مش .. أنا بجزمش أنه يكون مريض لكن شك فيه
+
+287
+00:22:04,310 --> 00:22:09,270
+أنه يكون مريضتمام؟ فبالتالي أي عينة تُأخد من شخص
+
+288
+00:22:09,270 --> 00:22:13,490
+suspected أنه يكون مريض فبالتالي بسميها clinical
+
+289
+00:22:13,490 --> 00:22:18,210
+sample عينة ال .. ال urine، ال bowel، عينة الدم،
+
+290
+00:22:18,210 --> 00:22:22,270
+عينة ال .. حتى ال .. ال .. الشعر، الأضافر، هذه
+
+291
+00:22:22,270 --> 00:22:27,730
+كلها عينات سواء الجسم، الانسجة، هذه كلها عينات
+
+292
+00:22:27,730 --> 00:22:30,770
+إحنا بناخدها من ناس شكين فيهم إن هم يكونوا مرضة أو
+
+293
+00:22:30,770 --> 00:22:35,480
+لأ و الفحوصات تثبت أو تنفي مرضهم، تمام؟فبالتالي
+
+294
+00:22:35,480 --> 00:22:39,880
+هذه بيسميها .. كلها clinical samples تمام؟ إيش
+
+295
+00:22:39,880 --> 00:22:43,820
+يعني medical laboratory science؟ إيش يعني تخصصنا؟
+
+296
+00:22:43,820 --> 00:22:49,020
+تخصصنا هو إن أنا أوظف أو أستخدم ال clinical
+
+297
+00:22:49,020 --> 00:22:54,780
+laboratory علشان إيش أعمل؟ أكتشف، أشخص، أراقب و
+
+298
+00:22:54,780 --> 00:22:59,660
+أعالج المرض تمام؟detect بدي أشوف هذا الشخص مريض
+
+299
+00:22:59,660 --> 00:23:04,920
+ولا لأ وبالتالي أ .. أ .. أعمله diagnosis و أراقب
+
+300
+00:23:04,920 --> 00:23:09,320
+حالة المريض أحيانا بيجيني مريض ممكن أطلب يطلع يعني
+
+301
+00:23:09,320 --> 00:23:12,820
+الدكتور يوجهه لعندي أعمله فحص في بداية الخطة
+
+302
+00:23:12,820 --> 00:23:16,880
+العلاجية في نص الخطة العلاجية برضه كمان أجريله فحص
+
+303
+00:23:16,880 --> 00:23:20,720
+تاني عشان أشوفه اتحسن ولا ماتحسنشطبعا و في نهاية
+
+304
+00:23:20,720 --> 00:23:23,040
+الخطة العلاجية ممكن اجريله فحص هذا بسميه
+
+305
+00:23:23,040 --> 00:23:27,800
+monitoring مراقبة طبعا و أيضا علشان نقدر احنا
+
+306
+00:23:27,800 --> 00:23:33,060
+نساهم indirectly بعلاج المرض طبعا احنا حكينا انا
+
+307
+00:23:33,060 --> 00:23:37,380
+مابساهم بشكل direct ولكن بشكل indirect طبعا ايش
+
+308
+00:23:37,380 --> 00:23:39,860
+نوع العينات اللي بتيجيني في ال clinical laboratory
+
+309
+00:23:39,860 --> 00:23:43,860
+قولنا clinical samples او clinical specimen طبعا
+
+310
+00:23:43,860 --> 00:23:48,380
+clinical specimen او clinical samples نفس المعنى
+
+311
+00:23:48,380 --> 00:23:55,410
+طبعاطيب إيش يعني؟ ممكن تكون دم blood، tissue تمام،
+
+312
+00:23:55,410 --> 00:23:59,050
+body fluid سواء الجسم، ممكن تكون دم أنتجة سواء
+
+313
+00:23:59,050 --> 00:24:03,470
+الجسم، أي نوع من .. أي عينة تأخد من شخص suspected
+
+314
+00:24:03,470 --> 00:24:07,050
+أنه يكون مريض بسميها clinical samples طبعا بحللها
+
+315
+00:24:07,050 --> 00:24:11,000
+كيميائيا، بعمللها chemical analysisو examination
+
+316
+00:24:11,000 --> 00:24:14,980
+علشان أشوف والله هذا الشخص عنده foreign organism
+
+317
+00:24:14,980 --> 00:24:20,080
+عنده مثلا جسم غريب أو كائن غريب زي بكتيريا، فطريات
+
+318
+00:24:20,080 --> 00:24:24,740
+وفيليات عملاله عدوى و اللي عنده مثلا abnormalities
+
+319
+00:24:24,740 --> 00:24:30,260
+وظائف كبد وظائف كيلة وظائف القلب وظائف الأعضاء
+
+320
+00:24:30,260 --> 00:24:34,060
+كلها أشوفها طبيعية ولا abnormal normal ولا
+
+321
+00:24:34,060 --> 00:24:39,210
+abnormal تمام هذا برضه كمانمن ضمن شغلنا كأخصائي
+
+322
+00:24:39,210 --> 00:24:44,250
+مختبرات طبية، كمان المعلومات اللي احنا حكينا بت ..
+
+323
+00:24:44,250 --> 00:24:49,250
+اللي بنستخدمها بتساعد الفريق الطبي عشان ياخد
+
+324
+00:24:49,250 --> 00:24:55,690
+قرارات تبعا لهذا المريضبتساعد طبعا انا لما بقدم
+
+325
+00:24:55,690 --> 00:25:00,430
+نتائج الفحوصات الطبيب بيصير .. تبقى .. بيربط
+
+326
+00:25:00,430 --> 00:25:03,350
+النتائج تبعتي حكينا بال history تبعت المريض
+
+327
+00:25:03,350 --> 00:25:06,210
+بالعلامات وال physical examination اللي ظاهرة على
+
+328
+00:25:06,210 --> 00:25:10,690
+المريض وبالتالي بياخد قرار ان هذا المريض مشخص بمرض
+
+329
+00:25:10,690 --> 00:25:16,920
+كذاو يسرف له العلاج كذا فبالتالي و برسمله الخطة
+
+330
+00:25:16,920 --> 00:25:21,820
+العلاجية طبعته خمسة و تمانين في المية من القرارات
+
+331
+00:25:21,820 --> 00:25:26,940
+الطبية تعتمد على ايش؟ على ال .. ال .. النتائج
+
+332
+00:25:26,940 --> 00:25:31,380
+المختبر تبعي او نتائج البحثات تبعتي اذا انا العين
+
+333
+00:25:31,380 --> 00:25:35,640
+العين الطبيب الثاني اللي بتشوف بنسبة خمسة و تمانين
+
+334
+00:25:35,640 --> 00:25:40,480
+في المية اما عين الطبيب aloneبتشوف بنسبة 15%، اذا
+
+335
+00:25:40,480 --> 00:25:45,020
+لا تستهينوا بتخصصكم، حبوا بتخصصكم، حبوا الاشي اللي
+
+336
+00:25:45,020 --> 00:25:49,680
+انتوا جايين تتعلموه و يكونوا فخورين بما تصنعوا لإن
+
+337
+00:25:49,680 --> 00:25:55,080
+انتوا تخصصكم لا يستهان فيه ولا يمكن الاستغناء عنه
+
+338
+00:25:55,450 --> 00:26:02,870
+يعني حتى الأطباء والصيادلة والطمريد لما بدهم ييجوا
+
+339
+00:26:02,870 --> 00:26:06,710
+يعملوا دراسات عليا لابد من إنهم يقوموا بإجراء
+
+340
+00:26:06,710 --> 00:26:12,320
+فحوصات وتجارب داخل مختبراتنا، تمام؟فبالتالي يعني
+
+341
+00:26:12,320 --> 00:26:18,580
+احنا تخصصنا كتير مهم و هذا يعني انه لا يقل أهمية
+
+342
+00:26:18,580 --> 00:26:22,180
+عن التخصصات الأخرى انا لا اقول ان التخصصات الأخرى
+
+343
+00:26:22,180 --> 00:26:26,620
+غير مهمة هي مهمنا و هي حجر أساس و لكن أيضا تخصصنا
+
+344
+00:26:26,620 --> 00:26:32,040
+مهم جدا و يجب ان انتوا تحبوا و تفتخروا فيه و تحسوا
+
+345
+00:26:32,040 --> 00:26:38,180
+قد جداش انتوا رح يكون دوركم فعال و قيم في عملية ال
+
+346
+00:26:38,180 --> 00:26:43,400
+health care providingعمليات تزويد الخدمات الصحية
+
+347
+00:26:43,400 --> 00:26:50,740
+لمستحقيها أو مستهلكيها what are lab tests؟ إيش
+
+348
+00:26:50,740 --> 00:26:55,600
+يعني الفحوصات الطبية؟ الفحوصات الطبية هي عبارة عن
+
+349
+00:26:55,600 --> 00:26:59,060
+ال medical procedures التجارب الطبية اللي من
+
+350
+00:26:59,060 --> 00:27:02,180
+خلالها اللي بدأ تطلب الحصول على عينة clinical
+
+351
+00:27:02,180 --> 00:27:05,920
+sample أو clinical specimen اللي هي عبارة عن blood
+
+352
+00:27:05,920 --> 00:27:10,090
+أو urine أو other tissueتمام؟ إذا ال .. ال ..
+
+353
+00:27:10,090 --> 00:27:13,630
+المصطلحات بتلاحظوا إنها مرتبطة في بعضها البعض و
+
+354
+00:27:13,630 --> 00:27:17,030
+تندرج تحت بعض، يعني بنعرف إيش يعني تخصص lab، بنصير
+
+355
+00:27:17,030 --> 00:27:19,870
+نفهم إيش يعني clinical lab أو medical lab، بنصير
+
+356
+00:27:19,870 --> 00:27:23,810
+نفهم إيش يعني lab test و إيش يعني clinical sample،
+
+357
+00:27:23,810 --> 00:27:29,250
+فكل هذه يعني معلومات مترابطة مع بعضها البعضطيب إيش
+
+358
+00:27:29,250 --> 00:27:33,930
+الدكتور هيستفيد من نتائج المختبر؟ طبعا الدكتور
+
+359
+00:27:33,930 --> 00:27:41,890
+دوره إنه يلاحظ، يعرف التغيرات اللي حاصلة على صحة
+
+360
+00:27:41,890 --> 00:27:46,450
+المريض طبعا ممكن خلال أو بعد أو قبل ظهور ال
+
+361
+00:27:46,450 --> 00:27:52,270
+symptoms الأعراض من خلال نتائج المختبرأيضا بتساعد
+
+362
+00:27:52,270 --> 00:27:57,070
+نتائج المختبر أو ال laboratory test بتساعد الربيب
+
+363
+00:27:57,070 --> 00:28:02,950
+انه يعمل diagnoses يشخص المرض يرسم خطة علاجية plan
+
+364
+00:28:02,950 --> 00:28:09,670
+patient treatment و أيضا يقيم وضع المريض هل استجاب
+
+365
+00:28:09,670 --> 00:28:14,190
+هل صار في عنده response او لألل treatment و أيضا
+
+366
+00:28:14,190 --> 00:28:19,350
+يراقب وضع المريض هالحال تتسوق أو تتحسن و هذا
+
+367
+00:28:19,350 --> 00:28:25,430
+الكلام ملخص في الجملة هذا الكلام كله هذا ملخص في
+
+368
+00:28:25,430 --> 00:28:30,130
+الجملة اللي احنا حكيناها detect, diagnose, monitor
+
+369
+00:28:30,130 --> 00:28:36,170
+and treat طبعا اذا انظروا detect بيكتشف ايش
+
+370
+00:28:36,170 --> 00:28:40,670
+التغيرات اللي حدثة على صحة المريضdiagnose بيشخص بي
+
+371
+00:28:40,670 --> 00:28:45,870
+treat بيعالج و أيضا بيعمل monitoring و evaluation
+
+372
+00:28:45,870 --> 00:28:53,570
+لحالة المريض طيب نتائج المختبر او نتائج الفحوصات
+
+373
+00:28:53,570 --> 00:28:59,710
+الطبية طبعا في عدة عوامل بتأثر عليها زي ash مثلا
+
+374
+00:28:59,710 --> 00:29:05,480
+زي ال gender ال gender ال sex و ال ash تمام؟إيش
+
+375
+00:29:05,480 --> 00:29:11,180
+يعني gender يعني بختلف؟ كيف يعني ال gender بتختلف
+
+376
+00:29:11,180 --> 00:29:17,700
+نتائج الفحوصات طبعا لإله؟ مثلا إحنا كإناس معروف
+
+377
+00:29:17,700 --> 00:29:23,160
+إنه نسبة ال hemoglobin عندنا في الدم من 11 ل 13لكن
+
+378
+00:29:23,160 --> 00:29:27,820
+ال .. ال .. الدكور ال males عندهم نسبة ال
+
+379
+00:29:27,820 --> 00:29:35,760
+homoglobin تمام من اربعتاش ل سبتاش او لسبعتاش طبعا
+
+380
+00:29:35,760 --> 00:29:40,940
+هم عندهم نسبة homoglobin اعلى طب ليش؟لأنه فيه فروق
+
+381
+00:29:40,940 --> 00:29:45,380
+تبعا لل sex أو ال gender طبعا .. طب .. احنا ك ..
+
+382
+00:29:45,380 --> 00:29:50,780
+كإناث احنا كل يوم .. كل شهر عندنا مقدار loss مقدار
+
+383
+00:29:50,780 --> 00:29:54,840
+فقدار من الدم من خلال ال administration تمام؟ و
+
+384
+00:29:54,840 --> 00:29:58,960
+عندنا أيضا ال .. ال muscle mass .. الكتلة الجسمية
+
+385
+00:29:58,960 --> 00:30:03,310
+عندنا أقل منهمفبالتالي هيكون عندنا نسبة ال
+
+386
+00:30:03,310 --> 00:30:09,370
+homoglobin أقل يعني احنا لإنه بنفقد blood بشكل
+
+387
+00:30:09,370 --> 00:30:14,550
+شهري و لإنه عندي عضلات أقل فانا محتاجة homoglobin
+
+388
+00:30:14,550 --> 00:30:19,030
+او بيكون عند ال homoglobin أقل هم عندهم ال body
+
+389
+00:30:19,030 --> 00:30:25,230
+mass أكترفمحتاجين أكتر فمحتاجين هموجلوبين أكتر لإن
+
+390
+00:30:25,230 --> 00:30:28,250
+الهموجلوبين هو اللي بيحمل ال oxygen بوصله للخلايا
+
+391
+00:30:28,250 --> 00:30:32,550
+تمام؟ عشان هيك نسبة الهموجلوبين عندهم أعلى من 14 ل
+
+392
+00:30:32,550 --> 00:30:37,290
+17 لكن إحنا عندنا الهموجلوبين من 11 ل 13، ليش؟أنا
+
+393
+00:30:37,290 --> 00:30:40,870
+بيصير عندي فقدان لل blood ب .. ب .. بفقد معاه
+
+394
+00:30:40,870 --> 00:30:45,790
+homoglobin و أيضا عندي عضلات أقل فمحتاجة oxygen
+
+395
+00:30:45,790 --> 00:30:50,610
+أقل فنسبة ال homoglobin عندي هتكون أقل من مقارنة
+
+396
+00:30:50,610 --> 00:30:57,630
+بالرجل اللي من عمري، تمام؟ طيب ال age أيضا بيأثر،
+
+397
+00:30:57,630 --> 00:31:05,720
+هلأ نسبة الموليد اللي عمرهممثلا عشرين .. sorry
+
+398
+00:31:05,720 --> 00:31:10,920
+عشرين يوم، عشر أيام أو حتى بالساعات، يعني الموليد
+
+399
+00:31:10,920 --> 00:31:15,920
+الصغيرة بالعمر أو الحديثي الولادة هدول بنلاحظ أن
+
+400
+00:31:15,920 --> 00:31:20,600
+الدم عندهم بيصل خمستاشر، سبعتاشر، عشرين بالنسبالي
+
+401
+00:31:20,600 --> 00:31:24,840
+ال homoglobinلكن مجرد مثلا ما يكبر يصير عمره سنة،
+
+402
+00:31:24,840 --> 00:31:27,260
+سنتين، بيرجع على الطبيعة، بيصير اتناش، تلاتاش،
+
+403
+00:31:27,260 --> 00:31:31,900
+احداش، زينا، زي ال adult طب ليش هذه الفوارق؟ لإن
+
+404
+00:31:31,900 --> 00:31:35,540
+هم أول ما ينولدوا بيكون .. بتكون ال .. لسه عمل
+
+405
+00:31:35,540 --> 00:31:40,560
+الرئتين مش كامل فبدهم كمية هموجلبين أكتر علشان
+
+406
+00:31:40,560 --> 00:31:45,500
+تحمي ال oxygen أكترهي هذا سبب السبب التاني هم
+
+407
+00:31:45,500 --> 00:31:49,120
+بيكونوا في حالة growth burst إيش يعني growth burst
+
+408
+00:31:49,120 --> 00:31:54,520
+انفجار في النمو تمام يعني في حالة نمو هائل يعني
+
+409
+00:31:54,520 --> 00:31:58,640
+تلاحظي أن الطفل حجمه هو عمره يوم غير عن حجمه عمره
+
+410
+00:31:58,640 --> 00:32:02,680
+شهر غير عن حجمه عمره شهرين تلاتة يعني معدل النمو
+
+411
+00:32:02,680 --> 00:32:06,740
+كتير عالي فهذا أيضا محتاج oxygen delivery فمحتاجهم
+
+412
+00:32:06,740 --> 00:32:12,110
+مجلبين أكترهذا سبب التاني و السبب التالت ان ال
+
+413
+00:32:12,110 --> 00:32:16,090
+homoglobin تبعهم نوعه .. نوع ال homoglobin اللي
+
+414
+00:32:16,090 --> 00:32:21,970
+عندهم بيكون بيمسك بال oxygen كتير ف .. فكقالية
+
+415
+00:32:21,970 --> 00:32:25,030
+تعويض ربنا عوضهم على ان هو بيمسك بال oxygen مبرداش
+
+416
+00:32:25,030 --> 00:32:30,130
+يتركه عند الخلايا مبرداش يوديله الخلايا يوصلهفربنا
+
+417
+00:32:30,130 --> 00:32:34,710
+يعوضهم انه يكون نسبة ال homoglobin عالية عشان انه
+
+418
+00:32:34,710 --> 00:32:37,990
+جد ما يمسك بال oxygen يكون في نسبة .. في نسبة
+
+419
+00:32:37,990 --> 00:32:43,710
+homoglobin جادرة توصلتمام؟ إذا هاي نتائج مختبر
+
+420
+00:32:43,710 --> 00:32:48,030
+اختلفت تبعا ل ال gender و ال age طبعا what you eat
+
+421
+00:32:48,030 --> 00:32:52,130
+and what you drink أكلتي سكريات كتير بيرتفع نسبة
+
+422
+00:32:52,130 --> 00:32:55,750
+السكر في الدم أكلتي دهون كتير أو كنتي لسه ماكلة
+
+423
+00:32:55,750 --> 00:33:00,330
+وجبة و بعدين أخدنا منك عينة blood و روحنا نفحصلك
+
+424
+00:33:00,330 --> 00:33:03,810
+دهون بنلاقي الدهون عالية لإن انت لسه يدهبك ماكلة
+
+425
+00:33:03,810 --> 00:33:08,110
+وجبة في بعض الفحوصات مثلا من .. من .. منشرط على
+
+426
+00:33:08,110 --> 00:33:11,320
+الشخص أنه لازم تيجي صايملأنها بتتأثر بالأكل
+
+427
+00:33:11,320 --> 00:33:16,060
+والشرب، في بعض الفحوصات بنشرط على المريض أو بنح ..
+
+428
+00:33:16,060 --> 00:33:20,400
+يعني بنعطي تعليمات للمريض إنه نوعيات معينة من
+
+429
+00:33:20,400 --> 00:33:24,180
+الأكل ممنوعة تاكلها، يعني مثلا في ف .. في فحص ال
+
+430
+00:33:24,180 --> 00:33:28,620
+adrenaline، هرمون ال adrenaline، لما أنا بدي أعمله
+
+431
+00:33:28,620 --> 00:33:33,000
+بحكي للمريض ماتيجيش تاكل أي إشي م .. م .. محتوط
+
+432
+00:33:33,000 --> 00:33:39,620
+عليه vanilla، تمام؟أي مشتقة لكريمة و ال cake و ال
+
+433
+00:33:39,620 --> 00:33:45,400
+ice cream و أي إشي يحتوي على vanilla ما .. مايجيش
+
+434
+00:33:45,400 --> 00:33:49,860
+ماكله المريض لإنه هيأثر على الفحص لإن المادة اللي
+
+435
+00:33:49,860 --> 00:33:53,800
+بقيسها بتشبه تركيب ال vanilla يعني بتأثر عليها
+
+436
+00:33:53,800 --> 00:33:58,940
+يعني فبالتالي إش إحنا بناكل، إش إحنا بنشرب أيضا
+
+437
+00:33:58,940 --> 00:34:04,380
+بتأثر على نتائج المختبر الأدوية اللي بناخدها أيضا
+
+438
+00:34:04,380 --> 00:34:08,270
+بتأثر على المختبراحيانا مثلا لما أنا بدي أجي أشوف
+
+439
+00:34:08,270 --> 00:34:13,970
+أعمل مزرعة لمريد لازم المريض يكون مش ماخد مضادات
+
+440
+00:34:13,970 --> 00:34:19,390
+حيوية لإنه إذا هو ماخد مضادات حيوية مش راح يبين
+
+441
+00:34:19,390 --> 00:34:24,670
+عندي بكتيريا مش راح تنمو البكتيريا أو الفطريات
+
+442
+00:34:24,670 --> 00:34:28,470
+مثلا اللي مسببة ال infection مسببة العدوى عنده
+
+443
+00:34:28,470 --> 00:34:33,000
+فبالتالي نتائج المزرعة راح تفشلفبالتالي ايضا بعض
+
+444
+00:34:33,000 --> 00:34:37,500
+الأدوية يحذر او يعني ما .. ما يحبذ انهم ياخدوها
+
+445
+00:34:37,500 --> 00:34:42,660
+قبل إجراء الفحص و بتأثر نتائج الفحصات بنوع الأدوية
+
+446
+00:34:42,660 --> 00:34:47,340
+اللي بياخدها المريض قبل إجراء الفحصو أيضا جدش هو
+
+447
+00:34:47,340 --> 00:34:51,240
+التابع التعليمات طبعا في بحوصات بيجي المريض أنا
+
+448
+00:34:51,240 --> 00:34:55,620
+بشرحله التعليمات كيف هو يجي .. مثلا في .. في عينات
+
+449
+00:34:55,620 --> 00:34:58,820
+أنا بجمعها من المريض يعني أنا مثلا باخد منه عينة
+
+450
+00:34:58,820 --> 00:35:03,960
+الدم انا باخد يعني او طبيب كمان ممكن ياخد منه
+
+451
+00:35:03,960 --> 00:35:11,600
+عينات سواء الجسمو عينات الأنسجات لكن في العينات ال
+
+452
+00:35:11,600 --> 00:35:15,240
+.. المريض بنفسه بيجمعها فبالتالي أنا لازم أعطي
+
+453
+00:35:15,240 --> 00:35:18,360
+تعليمات كيف يجمعها بشكل صحيح عشان أنا أجري فحص و
+
+454
+00:35:18,360 --> 00:35:23,460
+أخد نتائج صحيحة و بالتالي تكون النتائج فعالة
+
+455
+00:35:23,880 --> 00:35:28,380
+فبالتالي أيضا جدش هو التزم و اتبع ال .. ال
+
+456
+00:35:28,380 --> 00:35:34,060
+brightest instruction التعليمات ما قبل الفحص بي ..
+
+457
+00:35:34,060 --> 00:35:38,360
+بتكون الفحص ناجح التعليمات هذه كمان مش بس بيجمع
+
+458
+00:35:38,360 --> 00:35:42,220
+العينات يعني كيف هو يجمع العينة لأ برضه كمان بال
+
+459
+00:35:42,220 --> 00:35:45,980
+.. بالمعلومات السابقة يعني مثلا ماتاخدش مضادات
+
+460
+00:35:45,980 --> 00:35:49,280
+حيوية قبل إجراء الفحص إذا هو التزم أيضا الفحص
+
+461
+00:35:49,280 --> 00:35:53,580
+هينجحما تاكلش ما تشربش تعال صايم مثلا من 6 ل 8
+
+462
+00:35:53,580 --> 00:35:58,840
+ساعات أنواع معينة من الأكل لازم تتجنبها قبل إجراء
+
+463
+00:35:58,840 --> 00:36:04,200
+الفحص في فحوصات لازم ياكل إشي معين قبل إجراءه يعني
+
+464
+00:36:04,200 --> 00:36:10,840
+مثلا أحيانا عشان نشخص مشاكل الانسولين بعطي أنا
+
+465
+00:36:10,840 --> 00:36:15,780
+محلول سكري بخليه يشربه و بجري الفحص بعد نص ساعة
+
+466
+00:36:15,780 --> 00:36:22,400
+منه و بعد ساعة و بعد ساعتين فإذاتعليمات قبل إجراء
+
+467
+00:36:22,400 --> 00:36:30,320
+الفحص مهمة جدا وعلى أساسها بتختلف أو بتتأثر نتائج
+
+468
+00:36:30,320 --> 00:36:38,020
+الفحصات طبعاال .. ال .. نتائج المختبر أو فحوصات
+
+469
+00:36:38,020 --> 00:36:44,960
+المختبر بت .. بتورجيني قداش الشخص هذا normal او
+
+470
+00:36:44,960 --> 00:36:50,000
+abnormal يعني نتائجه إذا وقعت داخل ال normal range
+
+471
+00:36:50,000 --> 00:36:54,240
+معناها هو شخص normal أما إذا وقعت النتائج خارج ال
+
+472
+00:36:54,240 --> 00:36:58,040
+normal range أقل أو أعلى فهو شخص abnormal عنده
+
+473
+00:36:58,040 --> 00:37:03,460
+مشاكل ويجب أن تعالج طبعاالنتائج المختبر مابعطيهاش
+
+474
+00:37:03,460 --> 00:37:08,660
+قيمة والله مثلا نسبة ال homoglobin الطبيعية 11 لأ
+
+475
+00:37:08,660 --> 00:37:12,060
+غلط احكي هيك نسبة ال homoglobin الطبيعية عند
+
+476
+00:37:12,060 --> 00:37:15,920
+الإينات من 11 ل 13 و عند الرجال من 14 ل 16 او من
+
+477
+00:37:15,920 --> 00:37:21,000
+14 ل 17 هذا الكلام الصحيح لإن انا ممكن يكون دمي 11
+
+478
+00:37:21,000 --> 00:37:24,800
+و ازميلتي 11.5وزميلتي التانية اتناش وزميلتي
+
+479
+00:37:24,800 --> 00:37:29,940
+التالتة تلتاش وكلنا normal فبالتالي فيه فروق فردية
+
+480
+00:37:29,940 --> 00:37:34,840
+من شخص لآخر من حسب الكتلة الجسمية تبعته حسب
+
+481
+00:37:34,840 --> 00:37:40,580
+الاستجابة للهرمونات تبعته ومعدل نموه فبالتالي هذه
+
+482
+00:37:40,580 --> 00:37:44,200
+كلها بتأثر على نتائج المختبر فانا مابقدر احكي انه
+
+483
+00:37:44,200 --> 00:37:49,880
+النتيجة الطبيعية لفحص معين قيمة بحد ذاتها لأ
+
+484
+00:37:50,290 --> 00:37:55,030
+النتيجة الطبيعية لفحص هي لازم تكون واقعة في range
+
+485
+00:37:55,030 --> 00:38:00,210
+في مدى من إلى تمام لإنه في فروق فردية فعشان هيك
+
+486
+00:38:00,210 --> 00:38:03,890
+بنراعي الفروق الفردية و بنعتبر أنه normal يقع من
+
+487
+00:38:03,890 --> 00:38:10,130
+إلى أي شخص نتيجته خارج هذا المدى اللي من إلى
+
+488
+00:38:10,130 --> 00:38:14,730
+بنعتبره abnormal سواء أقل أو أعلى هو عنده مشكلة و
+
+489
+00:38:14,730 --> 00:38:21,350
+يجب أن تعالجطيب أنواع المختبرات التدبية في عندي
+
+490
+00:38:21,350 --> 00:38:27,050
+طبعا حسب موقعها إذا كانت داخل مستشفى attached to
+
+491
+00:38:27,050 --> 00:38:30,530
+hospital متصلة بالمستشفى فبسميها hospital
+
+492
+00:38:30,530 --> 00:38:37,010
+laboratory تمام؟ و هذه بتجري بحوصات على المرضى إذا
+
+493
+00:38:37,010 --> 00:38:41,110
+كان المختبر
+
+494
+00:38:41,110 --> 00:38:47,340
+مش متصل بمستشفى فبسميه privateأو community lab
+
+495
+00:38:47,340 --> 00:38:52,380
+طبعا مختبر خاص بيستلم عينات من ال .. من السواق كان
+
+496
+00:38:52,380 --> 00:38:56,400
+من الأطباء بيواجهوهم يعني الطبيب بيحكي للمريض روح
+
+497
+00:38:56,400 --> 00:39:00,700
+على المختبر يقولاني وعملي فحص كذا المريض نفسه
+
+498
+00:39:00,700 --> 00:39:03,940
+بيروح على المختبر وممكن يجري فحوصات عشان يطمن على
+
+499
+00:39:03,940 --> 00:39:10,040
+صحته في بعض الشركاتبتبع تعيينات لهذه المختبرات
+
+500
+00:39:10,040 --> 00:39:16,000
+الخاصة بحيث إنها هي تشتغلها و تقيم .. و تشوف هذه
+
+501
+00:39:16,000 --> 00:39:20,660
+الشركات تقيم شغل المختبرات الخاصة و على أساسها هذا
+
+502
+00:39:20,660 --> 00:39:26,560
+المختبر يرتقي أو إنه بياخد لفت نظرفبنقيم ال
+
+503
+00:39:26,560 --> 00:39:30,740
+quality تبعته من خلال ال .. هذا عشان .. عشان برضه
+
+504
+00:39:30,740 --> 00:39:34,860
+كمان انا اعطي ترخيص او اجددله ترخيص لازم برضه كمان
+
+505
+00:39:34,860 --> 00:39:40,880
+انا اختبر شغله، ايضا يجرى داخل ال private labs
+
+506
+00:39:40,880 --> 00:39:50,040
+يعني أبحاث طبية ممكن برضه كمان نشتغلهاإذا في عندي
+
+507
+00:39:50,040 --> 00:39:54,700
+نوعين حسب موقعهم ال laboratory ال hospital lab و
+
+508
+00:39:54,700 --> 00:39:57,860
+ال private lab اللي هو المختبر المستشفى و المختبر
+
+509
+00:39:57,860 --> 00:40:03,400
+الخاص طبعا في حاجة اسمها تدوير عينات a lot of
+
+510
+00:40:03,400 --> 00:40:06,680
+samples are sent between different labs for
+
+511
+00:40:06,680 --> 00:40:11,000
+uncommon testsيعني مثلا احنا فاتهين مختبر خاص
+
+512
+00:40:11,000 --> 00:40:15,300
+private lab على جدنا و ماعنديش كل الأجهزة متوفرة،
+
+513
+00:40:15,300 --> 00:40:19,820
+إجها مريض، المريض هذا بده فحص مثلا growth hormone،
+
+514
+00:40:19,820 --> 00:40:24,420
+فحص هرمون النمو، و هذا أنا مابقدر أوفرله يعنىفأنا
+
+515
+00:40:24,420 --> 00:40:29,680
+ممكن أخد عينة من المريض و بعدين أبعت العينة بشكل
+
+516
+00:40:29,680 --> 00:40:33,740
+أنا أتواصل من مختبر لمختبر آخر أبعت العينة على
+
+517
+00:40:33,740 --> 00:40:39,000
+مختبر آخر هو يعني عنده أجهزة أكتر تطوران و عنده
+
+518
+00:40:39,000 --> 00:40:42,520
+فحوصات أكتر بيجدم خدمات أكتر أبعتله إياها و هو
+
+519
+00:40:42,520 --> 00:40:48,880
+يشتغلها بحيث أنه في النهاية ثمن الفحص بيتقاسموا
+
+520
+00:40:48,880 --> 00:40:54,870
+المختبرين طبعا فإذا it's more cost effectiveانه
+
+521
+00:40:54,870 --> 00:41:00,390
+يعني ال .. بي .. بيأثر على سعر الفحص يعني انه
+
+522
+00:41:00,390 --> 00:41:03,650
+بيوفر في سعر الفحص مثلا ان مختبرات مثلا تسحب
+
+523
+00:41:03,650 --> 00:41:07,670
+العينة و مختبرات بتشتغل الشغل فتالي هي انهم هيك
+
+524
+00:41:07,670 --> 00:41:14,950
+بتقاسموا العينات و ال money ما بينهمطيب أنواع
+
+525
+00:41:14,950 --> 00:41:20,670
+المختبرات أو أقسام المختبرات تبعا لتخصيصيتها يعني
+
+526
+00:41:20,670 --> 00:41:25,130
+داخل المختبر لازم يكون في عندى أقسام في أقسام
+
+527
+00:41:25,130 --> 00:41:29,330
+مختلفة طبعا ان هم ال .. ال .. ال main department
+
+528
+00:41:29,330 --> 00:41:34,170
+الأقسام الأساسية تلاتة ولكن يتفرع تحتها أشياء أخرى
+
+529
+00:41:34,170 --> 00:41:37,950
+زى مثلا في قسم لازم يكون داخل المختبر اسم clinical
+
+530
+00:41:37,950 --> 00:41:42,900
+pathology اللي هو الأمراض السريريةطبعا هذا بي ..
+
+531
+00:41:42,900 --> 00:41:46,720
+بيجرى في كل فحوصات الهيماتولوجي، كل فحوصات
+
+532
+00:41:46,720 --> 00:41:51,400
+الانسجا، كل فحوصات الأمراض الخلاية وحتى ال routine
+
+533
+00:41:51,400 --> 00:41:56,720
+pathology يعني، فحوصات ال urine و ال .. ال stool
+
+534
+00:41:56,720 --> 00:42:02,450
+أيضا فيهمفي قسم ال microbiology اش يعني قسم ال
+
+535
+00:42:02,450 --> 00:42:05,770
+microbiology يعني قسم الأحياء الدقيقة اللى بيكشف
+
+536
+00:42:05,770 --> 00:42:09,250
+عن وجود بكتيريا ال bacteriology ال virology
+
+537
+00:42:09,250 --> 00:42:14,210
+الفيروسات ال mycology الفطريات الفطريات ال
+
+538
+00:42:14,210 --> 00:42:18,370
+parasitology طفيليات وحتى بعض فحوصات المناعة و
+
+539
+00:42:18,370 --> 00:42:23,760
+الأمثال بتكون بتجرى داخل هذا القسمقسم الكيميا أو
+
+540
+00:42:23,760 --> 00:42:28,760
+الكيميا الحيوية أو الكيميا الطبية بنجري فيه فحوصات
+
+541
+00:42:28,760 --> 00:42:32,160
+الكيميا يعني وظائف الأعضاء وظائف الكيلا وظائف
+
+542
+00:42:32,160 --> 00:42:37,100
+الكبد وكذا و أيضا فحوصات السكر و الدهون و كل هاي
+
+543
+00:42:37,100 --> 00:42:41,240
+الشغلات بنسميها biochemical tests تمام؟ و أيضا
+
+544
+00:42:41,240 --> 00:42:43,900
+فحوصات الهرمونات يجري داخل هذا القسم
+
+545
+00:42:46,480 --> 00:42:53,740
+طبعا بيتفرعوا كل قسم بيتفرع إلى فروع داخل القسم
+
+546
+00:42:54,720 --> 00:42:58,660
+طبعا في عندي منشأة اسمها ال blood bank، بنك الدم
+
+547
+00:42:58,660 --> 00:43:04,980
+طبعا هذا هو جسم منفرد بنفسه separate body يعني أو
+
+548
+00:43:04,980 --> 00:43:09,340
+منشأة مش منفردة بحد ذاتها يعني ممكن أنا ألاجي بنك
+
+549
+00:43:09,340 --> 00:43:13,380
+دم مالوش علاقة لا بمختبر خاص ولا بمستشفة زي بنك
+
+550
+00:43:13,380 --> 00:43:20,660
+الدم العام هذاطبعا؟ فهو ممكن يكون متصل أو لا يكون
+
+551
+00:43:20,660 --> 00:43:26,000
+متصل بمستشفى Bank الدم، طبعا؟ وإيش وظيفته؟ وظيفته
+
+552
+00:43:26,000 --> 00:43:32,020
+إن هو بيشتغل فحوصات سواء كان .. يعني بيتأكد إن
+
+553
+00:43:32,020 --> 00:43:36,310
+الدم نظيف مافيش فيه ..infectious disease يعني الدم
+
+554
+00:43:36,310 --> 00:43:39,230
+اللي هي .. اللي .. اللي احنا بناخده من المتبرعين و
+
+555
+00:43:39,230 --> 00:43:43,470
+بدنا نصرفه لالمستقبلين أول حاجة هو يتأكد من نظافته
+
+556
+00:43:43,470 --> 00:43:46,970
+مش فيه بكتيريا ولا فيروسات مثلا زي ال hepatitis
+
+557
+00:43:46,970 --> 00:43:52,570
+انها ممكن تقضي المستقبل و أيضا من خلال بيجري
+
+558
+00:43:52,570 --> 00:43:56,570
+فحوصات على عينا الوحدات الدم اللي بناخد .. طلقاها
+
+559
+00:43:56,570 --> 00:44:01,270
+من المتبرعين بنعرف فصيلة الدم ال blood groupنسبة
+
+560
+00:44:01,270 --> 00:44:05,070
+الدم أو نسبة ال homoglobin داخل الوحدة و أيضا مدى
+
+561
+00:44:05,070 --> 00:44:10,990
+تطابق ال .. ال .. الدم هذا يعني مثلا أجاني متبرع
+
+562
+00:44:10,990 --> 00:44:14,610
+أخدت منه وحدة دم وحدة الدم هذه في ال .. في ال
+
+563
+00:44:14,610 --> 00:44:17,090
+blood bank أنا بدأ أتأكد إنها نظيفة فاش فيها
+
+564
+00:44:17,090 --> 00:44:20,690
+فيروسات أو بكتيريا ممكن تسبب أمراض مرضية للمستقبل
+
+565
+00:44:20,690 --> 00:44:25,870
+بدأ أتأكد من وحدتها و نسبة ال homoglobin فيهاولمّا
+
+566
+00:44:25,870 --> 00:44:31,510
+يجيني مستقبل محتاج وحدة الدم هذه أخد منه عينة و
+
+567
+00:44:31,510 --> 00:44:35,610
+أجرب حصاد تطابق عشان أشوف هذه العينة مطابقة للدم
+
+568
+00:44:35,610 --> 00:44:39,790
+تبعه و بالتالي لو هو أخدها مش هتظهر عليه أعراض
+
+569
+00:44:39,790 --> 00:44:45,010
+ممكن ت .. تودي بحياته أو لأتمام؟ فإذا فحوصات
+
+570
+00:44:45,010 --> 00:44:50,110
+التطابق و الفصائل الدم و أيضا الفحوصات ل ال
+
+571
+00:44:50,110 --> 00:44:53,290
+infectious disease اللي بتجرى على وحدات الدم اللي
+
+572
+00:44:53,290 --> 00:44:57,310
+بتلقاها من المتبرعين هذه كلها بتجرى داخل ال blood
+
+573
+00:44:57,310 --> 00:45:02,530
+bank و نقولنا أن هو ممكن .. ممكن يكون أو لا يكون
+
+574
+00:45:02,530 --> 00:45:06,010
+متصل بمستشفى طيب
+
+575
+00:45:07,940 --> 00:45:12,180
+أنواع المختبر طبعا حسب ال service حسب الخدمة اللي
+
+576
+00:45:12,180 --> 00:45:17,840
+بيقدموها احنا لحظوا انه حسب المكان طبعه اذا كان
+
+577
+00:45:17,840 --> 00:45:20,440
+متصل في مستشفى بسميه hospital lab اذا ما .. اذا
+
+578
+00:45:20,440 --> 00:45:27,910
+ماكان يعني private فبسميه private labأقسام المختبر
+
+579
+00:45:27,910 --> 00:45:32,410
+و أيضا أنواعه حسب الخدمة اللي بيقدموها، في حاجة
+
+580
+00:45:32,410 --> 00:45:35,130
+بسميها community business lab يعني مختبر الرعاية
+
+581
+00:45:35,130 --> 00:45:40,530
+الأولية بجري بحوصات بسيطة جدا اللي هي يعني ال ..
+
+582
+00:45:42,740 --> 00:45:46,840
+فحوصات urine و stool و نسبة الدم و يعني فحوصات
+
+583
+00:45:46,840 --> 00:45:53,980
+بسيطة جدا district hospital lab يعني هو مثلا زي
+
+584
+00:45:53,980 --> 00:45:58,800
+الفحوصات اللي بتكون موجودة في المدينة يعني مثلا
+
+585
+00:45:58,800 --> 00:46:02,160
+انا عندي قرية تابعة على مدينة هذه القرية فيها
+
+586
+00:46:02,160 --> 00:46:07,380
+مختبر صغيرالمختبر الصغير هذا إله district hospital
+
+587
+00:46:07,380 --> 00:46:12,400
+مختبر آخر بعيد عنه موجود في المدينة متصل معاه يعني
+
+588
+00:46:12,400 --> 00:46:17,560
+في تواصل ما بينهم في communication إجت عينة بتتطلب
+
+589
+00:46:17,560 --> 00:46:21,540
+فحوصات دقيقة ومتطورة بيرسلوها على ال district
+
+590
+00:46:21,540 --> 00:46:25,820
+hospital لاب علشان يشتغلوها، تمام؟ فإذا ال
+
+591
+00:46:25,820 --> 00:46:29,160
+district hospital lab زي الفحص .. زي المختبر اللي
+
+592
+00:46:29,160 --> 00:46:33,160
+في المدينة تابع لمختبر في القرية، تمام؟ال regional
+
+593
+00:46:33,160 --> 00:46:39,980
+hospital lab يعني مثلا زي مختبر اللي مثلا عندي عدة
+
+594
+00:46:39,980 --> 00:46:45,240
+مدن إلها مختبر مرجع في العاصمة، تمام؟ يعني أكبر
+
+595
+00:46:45,240 --> 00:46:49,220
+شوية من ال district hospital lab و أيضا أو موجود
+
+596
+00:46:49,220 --> 00:46:53,800
+في إقليم معين أو في منظومة معينة فبالتالي بيكون
+
+597
+00:46:53,800 --> 00:46:58,660
+مرجع له عدة مختبرات أصغر منهال central أو ال
+
+598
+00:46:58,660 --> 00:47:02,200
+public health lab زي مختبرات منظمة الصحة العالمية
+
+599
+00:47:02,200 --> 00:47:07,160
+طبعا هذا إيش بتشرف على مختبر مش على جد قرى و مدن
+
+600
+00:47:07,160 --> 00:47:12,260
+لأ على جد دول تمام و بتتابع شغل ال public health
+
+601
+00:47:12,260 --> 00:47:18,700
+إيش يعني؟ يعني بتتابع مستوى الصحة العامة ليعني
+
+602
+00:47:18,700 --> 00:47:25,320
+شريحة كتير كبيرة من الناسطيب إيش يعني lab
+
+603
+00:47:25,320 --> 00:47:28,260
+informatics؟ ال lab informatics طبعا اللي جيتها
+
+604
+00:47:28,260 --> 00:47:32,380
+الزمان كان شغل المختبرات النتائج يجيني طلب الفحص
+
+605
+00:47:32,380 --> 00:47:37,020
+على ورقة أنا أجري الفحص و أسلم النتائج أيضا على
+
+606
+00:47:37,020 --> 00:47:40,900
+ورقة أخرى و أبعتها فكان في تبادل ورق ما بين
+
+607
+00:47:40,900 --> 00:47:45,380
+الأقسام هلأ إحنا بنشوف كل شي صار محاوسب حتى
+
+608
+00:47:45,380 --> 00:47:51,500
+تعليمنا الأيام هذه صار محاوسب فبالتالي ال .. ال ..
+
+609
+00:47:51,970 --> 00:47:56,750
+ال lab informatics هو عبارة عن منظومة محوثبة من
+
+610
+00:47:56,750 --> 00:48:02,390
+خلالها بيتم تبادل exchange لل data هي عبارة عن
+
+611
+00:48:02,390 --> 00:48:06,350
+software program and computer هي برامج وأجهزة
+
+612
+00:48:06,350 --> 00:48:10,910
+computer بي .. وظيفتها إيش تعملي exchange لل data
+
+613
+00:48:11,650 --> 00:48:16,530
+طبعا data لاقاش يعني معلومات لاقاش اللي بنتبدلها
+
+614
+00:48:16,530 --> 00:48:21,690
+حسب عن المرضى و الفخصات اللي انطلبت لهم و النتائج
+
+615
+00:48:21,690 --> 00:48:26,270
+اللي اتسجلت لهم و هذه كلها بسميها lab informatics
+
+616
+00:48:26,270 --> 00:48:31,770
+systemطيب مثال عليها، هلأ بيجي .. هلأ صار احنا
+
+617
+00:48:31,770 --> 00:48:34,410
+بيجينا في المختبرات .. هذا الاشي اللي ان شاء الله
+
+618
+00:48:34,410 --> 00:48:38,150
+لما تروحوا على مستشفيات و تتدربوا، راح تشوفوه
+
+619
+00:48:38,150 --> 00:48:42,510
+بعينكم طلب الفحص بيجي من خلال إشعار على كمبيوتر
+
+620
+00:48:42,510 --> 00:48:50,870
+المختبر، تمام؟ال .. المختبر .. بيجي الكاتب بيفوت
+
+621
+00:48:50,870 --> 00:48:56,630
+على الطلبات الفحوصات و بيعرف من خلالها أخصاء
+
+622
+00:48:56,630 --> 00:49:00,770
+المختبر إيش الفحوصات المطلوبة على العينات و
+
+623
+00:49:00,770 --> 00:49:05,870
+بيجريهاطبعا طلعت النتائج على نفس الطلب اللي إجا
+
+624
+00:49:05,870 --> 00:49:10,410
+على الكمبيوتر بيجي أخسها المختبر و بيسجل النتائج
+
+625
+00:49:10,410 --> 00:49:13,490
+اللي طلعت لكل عينة كل عينة إلها بيكون طلب على
+
+626
+00:49:13,490 --> 00:49:18,730
+الكمبيوتر فبعبيها و باعتمدها الدكتور بيروح بيفتح
+
+627
+00:49:18,730 --> 00:49:25,900
+على نفس الملفتمام؟ و بيقرأ نتائج المختبر فبالتالي
+
+628
+00:49:25,900 --> 00:49:31,500
+بيشخص و بيكتب علاج على ال computer أيضا و
+
+629
+00:49:31,500 --> 00:49:36,540
+بيعتمدوا، بيروح الصيدلاني بيفتح على ال .. نفس
+
+630
+00:49:36,540 --> 00:49:41,410
+الملف للمريضوبيشوف أشي العلاج المطلوب، بيوصف
+
+631
+00:49:41,410 --> 00:49:45,830
+وبيعطي إله، تمام؟ الكاتب بده يعطي موعد لصورة أشعة
+
+632
+00:49:45,830 --> 00:49:53,210
+أو موعد لفحص يعني تصوير، أي فحصرانين، أي إشي من
+
+633
+00:49:53,210 --> 00:49:57,550
+خلال أنه يعني بيفوت على صفحة ال .. على ملف المريض
+
+634
+00:49:57,550 --> 00:50:02,700
+وبيكتبله الموعد داخل هذا الملفأصبح المريض صار
+
+635
+00:50:02,700 --> 00:50:06,460
+عندنا عبارة عن إيش؟ عبارة عن رقم ملف لازم يكون هو
+
+636
+00:50:06,460 --> 00:50:11,300
+المريض محتفظ فيه و من خلاله إحنا بنفوت على هذا
+
+637
+00:50:11,300 --> 00:50:17,460
+الملف بنلاقي كل المعلومات كله بيسجل معلوماته على
+
+638
+00:50:17,460 --> 00:50:21,060
+هذا الملف فصار إيش في عندي? exchange that data أنا
+
+639
+00:50:21,060 --> 00:50:26,380
+لما بحصل على رقم المريض الطبي رقم ملفه بفوت على
+
+640
+00:50:26,380 --> 00:50:29,600
+هذا البرنامج على ال software program و ال computer
+
+641
+00:50:30,360 --> 00:50:34,460
+و من خلالها بعرف إيش انطلب له فحوصات، إيش أنا سجلت
+
+642
+00:50:34,460 --> 00:50:37,960
+نتائج، إيش الدكتور كتاب له علاج، إيش القصيدلاني
+
+643
+00:50:37,960 --> 00:50:42,360
+صرف له علاج، طبع السكرتارية الطبية إمتى أعطاه موعد
+
+644
+00:50:42,360 --> 00:50:48,380
+مراجعة، كإمتى انكتبله موعد صور أشعة، إمتى انكتبله
+
+645
+00:50:48,380 --> 00:50:53,280
+موعد مثلا جلسات علاج طبيعي، و هكذا، كلها هذه عبارة
+
+646
+00:50:53,280 --> 00:51:00,020
+عن إيش بنسميها informatics، تمام؟الجزء اللى بيتعلق
+
+647
+00:51:00,020 --> 00:51:04,900
+بشغلى انا مثلا امتى اعطانى عينة و امتى انا صح
+
+648
+00:51:04,900 --> 00:51:08,860
+اشتغلتها و امتى سجلت نتائجى و اش النتائج اللى
+
+649
+00:51:08,860 --> 00:51:13,220
+سجلتها و اش الفحوصات اللى كانت مطلوبة بسميها lab
+
+650
+00:51:13,220 --> 00:51:17,920
+informatics لإنها معلومات بتخص شغلى انا بتخص شغلى
+
+651
+00:51:17,920 --> 00:51:22,940
+ك lab فبسميها lab informatics طبعا هى معناه اش
+
+652
+00:51:22,940 --> 00:51:25,400
+يعنى ال informatics و اش يعنى ال lab informatics
+
+653
+00:51:25,400 --> 00:51:31,620
+systemتمام؟ طيب فهذا ال system إيش .. إيش مميزاته
+
+654
+00:51:31,620 --> 00:51:37,480
+أصبح يسهل عمل المختبر،
+
+655
+00:51:37,480 --> 00:51:42,170
+صار في عندي سهولة لطلب الفحوصات لكل مريضصار في
+
+656
+00:51:42,170 --> 00:51:45,290
+عندى tracking، إيش يعني tracking؟ يعني متابعة،
+
+657
+00:51:45,290 --> 00:51:50,090
+أشوف المريض وصلت العينة للمختبر ولا لأ، إشتغلوها
+
+658
+00:51:50,090 --> 00:51:53,350
+أو لأ، طلعت النتائج، سجلوا النتائج أو لأ، هذه كلها
+
+659
+00:51:53,350 --> 00:51:55,930
+عبارة عن متابعة من خلال إن أنا ضغطت زر، بس أشوف
+
+660
+00:51:55,930 --> 00:52:00,390
+ملف المريض إيش انكتب عليه معلومات أو بياناتو أيضا
+
+661
+00:52:00,390 --> 00:52:04,490
+تضمن help guarantee a better quality for result
+
+662
+00:52:04,490 --> 00:52:09,330
+يعني بتضمنلي انه انا يكون في عندى جودة عالية
+
+663
+00:52:09,330 --> 00:52:13,870
+للنتائج بدل ما انا اضل اكتب على ورق و الورق هذا
+
+664
+00:52:13,870 --> 00:52:21,730
+يضيع يتلف نفقدها فبالتالي نضيع النتائج تبعتنا
+
+665
+00:52:21,730 --> 00:52:27,710
+بتساعد الدكاترة من ان هم ايشيعملوا تشريك على ما تم
+
+666
+00:52:27,710 --> 00:52:30,930
+كتابة على ملف المريض وبالتالي بتساعدهم في التشخيص
+
+667
+00:52:30,930 --> 00:52:38,030
+ووصف العلاج. هك بنكون خلصنا جزئية ال medical
+
+668
+00:52:38,030 --> 00:52:41,410
+laboratories. طبعا تعرفنا إيش يعني مختبر، إيش يعني
+
+669
+00:52:41,410 --> 00:52:45,430
+clinical أو medical laboratory وإيش إحنا وظيفتنا
+
+670
+00:52:45,430 --> 00:52:49,910
+وأنواع المختبرات مع أقسامها ونوع الخدمات اللي
+
+671
+00:52:49,910 --> 00:52:57,430
+بتقدمها داخل المختبرات.هنحكي جزئية بسيطة عن ال ..
+
+672
+00:52:57,430 --> 00:53:04,710
+ال lab safety طبعا جزئية بسيطة جدا ه .. نختصرها ..
+
+673
+00:53:04,710 --> 00:53:08,770
+يعني هذه مختصرة موضوع المحاضرة العملية هيكون
+
+674
+00:53:08,770 --> 00:53:14,810
+باسهاب أكتر عنها طبعا ال lab safety ruleقواعد
+
+675
+00:53:14,810 --> 00:53:19,670
+السلام والأمان داخل المختبر طبعا هي اشي مهم جدا هي
+
+676
+00:53:19,670 --> 00:53:26,190
+major aspect اشي مهم جدا في كل مختبر كل طالب طبعا
+
+677
+00:53:26,190 --> 00:53:32,530
+في المختبر لازم يتبع القواعد السلام والأمان لكل
+
+678
+00:53:32,530 --> 00:53:37,890
+تجربة بيجريهاطبعا ال lab safety rule من القواعد
+
+679
+00:53:37,890 --> 00:53:42,610
+السلامة و الأمان حتى العلامات و الإشارات احنا
+
+680
+00:53:42,610 --> 00:53:50,110
+أحيانا بنكون كاتبين ورق معالجينه او رموز على ال ..
+
+681
+00:53:50,110 --> 00:53:54,650
+معالجينه على ال أنابيب على ال bottles الذجاجات
+
+682
+00:53:54,650 --> 00:53:59,130
+طبعتناعلى الحيط على الخزانات عشان انحذر ان هنا في
+
+683
+00:53:59,130 --> 00:54:03,170
+اشي حارق هنا في اشي ممكن يأذي الشخص هنا اشي ..
+
+684
+00:54:03,170 --> 00:54:09,350
+فبالتالي لازم احنا ايضا نكون بنعرف هاي الشغلات و
+
+685
+00:54:09,350 --> 00:54:13,410
+بنتابعها سواء كانت قواعد او ان انا التزم
+
+686
+00:54:13,410 --> 00:54:16,790
+بالتعليمات اللي مكتوبة على الخزانة مثلا مش لازم
+
+687
+00:54:16,790 --> 00:54:20,350
+انا .. اي اشي داخل الخزانة هادى من هو انا مش لابسة
+
+688
+00:54:20,350 --> 00:54:23,270
+gloves فبالتالي انا بدى اتبع هاي الشغلات ليش؟ عشان
+
+689
+00:54:23,270 --> 00:54:27,100
+أحمي نفسيو أحمي ال classmates تبعوني زملائي اللي
+
+690
+00:54:27,100 --> 00:54:31,220
+داخل الفصل أو داخل المحاضرة طبعا إيش هي ال lab
+
+691
+00:54:31,220 --> 00:54:37,020
+safety rules بشكل عام؟أنا داخل المختبر لازم ألتزم
+
+692
+00:54:37,020 --> 00:54:41,360
+بإن أنا ألبس ال lab coat لأ ألبس ال gloves و
+
+693
+00:54:41,360 --> 00:54:45,380
+أحيانا بعد ال .. ال .. العمل المخبر يحتاج إن أنا
+
+694
+00:54:45,380 --> 00:54:50,920
+ألبس goggles نضارات و ألبس shoes تمام صندل لأ كعب
+
+695
+00:54:50,920 --> 00:54:57,660
+لأ الأشياء اللي ت .. اللي هتتحقق معها الأناقة مش
+
+696
+00:54:57,660 --> 00:55:01,220
+ضرورية داخل المختبر أناقتك بي close shoes لازم
+
+697
+00:55:01,220 --> 00:55:05,570
+تكون موجودةيعني أنا بدي .. بدي أناقتي ولا سلامتي؟
+
+698
+00:55:05,570 --> 00:55:09,590
+لأ سلامتي أبدا من الأناقة، تمام؟ فبالتالي لازم انه
+
+699
+00:55:09,590 --> 00:55:14,310
+ال shoes يكون مغلق، فال gloves اشي .. اشي مهم
+
+700
+00:55:14,310 --> 00:55:18,210
+تمام؟ ال lab coat مطلوب، ال goggles حكينا بعض
+
+701
+00:55:18,210 --> 00:55:23,100
+الشغل محتاجة يعني إذا كانالتفاعل متطاير أو ممكن
+
+702
+00:55:23,100 --> 00:55:27,280
+يعطي شرر أو ممكن يعطي رزاد فبالتالي أنا لازم ألبس
+
+703
+00:55:27,280 --> 00:55:32,700
+goggles نضارات علشان أحمي نفسي طبعا ال issues لازم
+
+704
+00:55:32,700 --> 00:55:38,740
+يكون مغلق تمام مش لازم يكون مفتوح و الأشياء
+
+705
+00:55:38,740 --> 00:55:43,140
+المريحة و العملية و الأنيقة و ال .. ال .. ال new
+
+706
+00:55:43,140 --> 00:55:47,560
+fashion هاي نتركنا منها أنا قدنا نحققها ان شاء
+
+707
+00:55:47,560 --> 00:55:50,080
+الله برا المختبر و داخل المختبر سلامتنا أو لا
+
+708
+00:55:52,070 --> 00:55:58,370
+الغوايش طبعا don't wear غوايش ماشي ال .. ال ..
+
+709
+00:55:58,370 --> 00:56:01,770
+الناس اللي معودة نفسها على ال accessories
+
+710
+00:56:01,770 --> 00:56:06,570
+الاكسسوارات و مابتطلعش من غيرها هتلاقي حالها ان هي
+
+711
+00:56:06,570 --> 00:56:12,850
+بتتغلب داخل المختبر فبالتالي مش لازم ان هي ت ..
+
+712
+00:56:12,850 --> 00:56:16,970
+يعني تلبسها و أيضا الملابس الواسعة و الفطفادة الكم
+
+713
+00:56:16,970 --> 00:56:21,780
+اللي نازل زي الفراشة و ال ..و الملابس الواسعة و
+
+714
+00:56:21,780 --> 00:56:26,680
+الشيفونات و الحاجات هذه برضه كمان مش مطلوبة طبعا
+
+715
+00:56:26,680 --> 00:56:31,920
+مش لازم انا يعني شايفين هذا الشخص قاعد بيشتغل في
+
+716
+00:56:31,920 --> 00:56:34,960
+منطقة هذه .. هذه اسمها safety cabinets يعني اللي
+
+717
+00:56:34,960 --> 00:56:38,180
+بيفوت جوا لازم يكون معقم و لا و أحيانا بتكون ..
+
+718
+00:56:38,180 --> 00:56:40,820
+بنكون بنشتغل في أشياء معضية داخل .. في الداخل هذه
+
+719
+00:56:40,820 --> 00:56:43,910
+ال cabinetتمام؟ فهو .. هو لابس ال gloves يعني كان
+
+720
+00:56:43,910 --> 00:56:47,110
+متعامل مع الأشياء اللي في الداخل مسك التليفون
+
+721
+00:56:47,110 --> 00:56:50,650
+الخاص فيه و هذا غلط فأنا .. و أنا لابسة ال gloves
+
+722
+00:56:50,650 --> 00:56:54,950
+و أنا بشتغل في .. يعني لمست العينات و لمست الأدوات
+
+723
+00:56:54,950 --> 00:56:59,010
+حتى المواد الكيميائية مش لازم أرجع في نفس ال .. ال
+
+724
+00:56:59,010 --> 00:57:03,850
+gloves أو في نفس الأيدالمس الأقلام و التليفونات و
+
+725
+00:57:03,850 --> 00:57:12,170
+حتى مقابد الغرف مش لازم طبعا يفضل إن أنا أخلع ال
+
+726
+00:57:12,170 --> 00:57:16,170
+gloves و أغسل إيديا و بعدين أروح أخد أغراضي و إلا
+
+727
+00:57:16,170 --> 00:57:20,950
+بكون أنا ساعتها أشبع باخد مواد معضية أو مواد سامة
+
+728
+00:57:20,950 --> 00:57:24,110
+و بروح بحطها على أغراضي علشان أنشرها بعد هيك بعد
+
+729
+00:57:24,110 --> 00:57:28,350
+ما أطلع المختبر أنشرها everywhereطبعا أعطى زميلاتى
+
+730
+00:57:28,350 --> 00:57:31,390
+شوية من تخصصى و زميلاتى اللى من التخصص التانى
+
+731
+00:57:31,390 --> 00:57:37,190
+أعطهم شوية مواد .. مواد سامة و .. معدية و هكذا
+
+732
+00:57:37,190 --> 00:57:43,230
+يعني بكون انا ايهاش برج ارسال بنشر هذا غلط طبعا
+
+733
+00:57:43,230 --> 00:57:47,580
+لازم ناخد حضرناالأقلم طبعا إذا أنا بكتب فيها و هي
+
+734
+00:57:47,580 --> 00:57:51,680
+موجودة عند الأوضاع المختبر مش لازم أحطها بالثم أو
+
+735
+00:57:51,680 --> 00:57:56,020
+إن أنا خلاص يعني حاسيت إن الجلم اتلوث ما .. يعني
+
+736
+00:57:56,020 --> 00:58:02,450
+ما أرجع أحطه مع باقي أغراضي أو داخل الشنطةمااتريكش
+
+737
+00:58:02,450 --> 00:58:07,510
+ال benz burner شغال و أروح أتحدث أو أ .. أتفسح أو
+
+738
+00:58:07,510 --> 00:58:12,290
+أ .. أروح في .. أشغل شغل تاني لإنه ممكن يعني
+
+739
+00:58:12,290 --> 00:58:16,890
+يتأيدي بحرائق تمام؟ أول ما أخلص شغلي بسكر ال benz
+
+740
+00:58:16,890 --> 00:58:20,590
+burner اللي هو لها ال benz علشان أضمن إنه مايكونش
+
+741
+00:58:20,590 --> 00:58:24,830
+في عندي حرائق و طبعا أتأكد إنه الغاز المقبض
+
+742
+00:58:24,830 --> 00:58:28,010
+الرئيسي للغاز أنا كمان أغلقته عشان مايصيرش في عندي
+
+743
+00:58:28,010 --> 00:58:32,270
+تسريب غاز لإنه في حوادث كتيرة صارتانه مثلا يكون
+
+744
+00:58:32,270 --> 00:58:35,250
+فيه تسريب للغاز مع إضاءة الكهربا يصير في حرائق.
+
+745
+00:58:36,170 --> 00:58:39,070
+طبعا الناس اللي .. الحمد لله نعمة الإسلام احنا
+
+746
+00:58:39,070 --> 00:58:46,090
+بنغطي شعارنا ولكن الناس اللي مش محجبة لازم ما تترك
+
+747
+00:58:46,090 --> 00:58:52,690
+الشعر سايح وسايب لأ يفضل ربطه او رفعه علشانمايكونش
+
+748
+00:58:52,690 --> 00:58:56,530
+مش .. يعني مثلا مايحترقش او يصير فيه contamination
+
+749
+00:58:56,530 --> 00:59:02,910
+فيصير في خطر hazard او contamination تمام؟ don't
+
+750
+00:59:02,910 --> 00:59:07,150
+food or drink water in the lab مش لازم ناكل او
+
+751
+00:59:07,150 --> 00:59:12,330
+نشرب داخل المختبر او بجانب اماكن العمل لإنه ممكن
+
+752
+00:59:12,330 --> 00:59:16,230
+تتلوث هاي المأكولات و المشروبات و بالتالي انه نؤذي
+
+753
+00:59:16,230 --> 00:59:20,960
+أنفسناprotect your safety و أيضا كمان مش لازم ان
+
+754
+00:59:20,960 --> 00:59:24,640
+انا يجي على بالي هيك من الفضول اتذوق اشي داخل
+
+755
+00:59:24,640 --> 00:59:29,860
+المختبر protect your hand safety لازم مثلا لما انا
+
+756
+00:59:29,860 --> 00:59:34,880
+ألمس اشي ساخنمثلا ال flask ساخن ألبس gloves عازل
+
+757
+00:59:34,880 --> 00:59:41,820
+للحرارة أو أمسكه من خلال ملقط تمام؟ كيف أيضا ب ..
+
+758
+00:59:41,820 --> 00:59:46,820
+بحافظ على ال .. ال .. إيديا سلامة إيديا أن أنا ما
+
+759
+00:59:46,820 --> 00:59:51,140
+أمسك الأشياء ال .. الزجاج المكسور أو الأدوات
+
+760
+00:59:51,140 --> 00:59:54,940
+الحادة بإيدي على طول لأ لازم أن أنا أتعامل معها
+
+761
+00:59:54,940 --> 01:00:00,510
+بحذر أيضا أتابع أعمل wash hand باستمرارهذا أيضا
+
+762
+01:00:00,510 --> 01:00:05,370
+بيحافظلي على سلامة اليدان ال electrical safety هو
+
+763
+01:00:05,370 --> 01:00:10,710
+أول ما بخلص شغل بجهاز computer بفصل الكبش تبعه من
+
+764
+01:00:10,710 --> 01:00:19,430
+الكهربا و طبعا ممنوع ألامس أو أتركالأسلاك والأحبال
+
+765
+01:00:19,430 --> 01:00:24,690
+الكهربائية مكشوفة طبعا ال chemical safety مش لازم
+
+766
+01:00:24,690 --> 01:00:33,030
+أنا ألمس أحس أو أشم أو أتذوق أي مادة كيميائية داخل
+
+767
+01:00:33,030 --> 01:00:39,500
+المختبر تمام؟ و أيضا مش لازمأخلط مواد كيميائية هيك
+
+768
+01:00:39,500 --> 01:00:43,200
+يطلع فيا حس ال .. الحس الكيميائي عندي و أبلش أخلط
+
+769
+01:00:43,200 --> 01:00:47,020
+مواد كيميائية مدام أنا ما .. ما أعطيتكم تعليمات
+
+770
+01:00:47,020 --> 01:00:52,280
+أنه اضيفي مادة ألف على بها انت ما تعملي إضافة،
+
+771
+01:00:52,280 --> 01:00:55,550
+تمام؟لأن خلط المواد الكيميائية مع بعضها البعض ممكن
+
+772
+01:00:55,550 --> 01:00:59,430
+تكون نتائجه يعني أو عواقب وخيمة keep lids on
+
+773
+01:00:59,430 --> 01:01:04,830
+chemical container طبعا المواد sorry المغطية لازم
+
+774
+01:01:04,830 --> 01:01:08,130
+انا اترك الغطاية عليها keep lids ال lids يعني
+
+775
+01:01:08,130 --> 01:01:11,090
+الغطاة لازم اضل احط .. احطها على ال chemical
+
+776
+01:01:11,090 --> 01:01:14,290
+container علشان في النهاية في مواد ممكن تمتسر
+
+777
+01:01:14,290 --> 01:01:19,590
+وطوبها فيتغير تركيزهافي مواد ممكن أنها هي تمتص
+
+778
+01:01:19,590 --> 01:01:25,410
+الوطوبة فتصير مشتعلة، في مواد ممكن تتبخر فبالتالي
+
+779
+01:01:25,410 --> 01:01:28,530
+أنا لازم أضل حاطة ال lids اللي هي الغطايا على ال
+
+780
+01:01:28,530 --> 01:01:33,390
+container يعني أسكر منيهاdon't take any culture
+
+781
+01:01:33,390 --> 01:01:38,350
+out of the lab إذا أنا بشتغل بمزارع تمام مش لازم
+
+782
+01:01:38,350 --> 01:01:43,570
+هذه أطباق عبارة عن مزرعة فيها مواد مغذية و بتنمو
+
+783
+01:01:43,570 --> 01:01:46,930
+عليها البكتيريا أو الفطريات و من خلالها أنا بتعرف
+
+784
+01:01:46,930 --> 01:01:52,410
+على الفطريات أو البكتيريا اللي ممكن تسبب عدوى تمام
+
+785
+01:01:52,410 --> 01:01:57,810
+ف .. و هذه أيضا عبارة عن culture ولكن هنا مثلا
+
+786
+01:01:57,810 --> 01:02:01,590
+المادة الغذائية صلبة هنا المادة الغذائية سائلةطبعا
+
+787
+01:02:01,590 --> 01:02:07,330
+هذه
+
+788
+01:02:07,330 --> 01:02:10,810
+ال cultures هذه المواد المغذية اللي نامي عليها
+
+789
+01:02:10,810 --> 01:02:16,270
+بكتيريا أنا ممنوع أخدها برا المختبر طبعا؟ ليش؟
+
+790
+01:02:16,270 --> 01:02:21,050
+علشان ممكن تنتوج و تنكسر يعني في النهاية تنتشر
+
+791
+01:02:21,050 --> 01:02:27,060
+العدوى خارج المختبر لأ فبالتالي لازمسواء كانت ال
+
+792
+01:02:27,060 --> 01:02:31,840
+.. ال .. نتيجة ال .. يعني نتيجة المزرعة هاي
+
+793
+01:02:31,840 --> 01:02:35,780
+البكتيريا اللي نمت عندى معضية أو غير معضية بتسبب
+
+794
+01:02:35,780 --> 01:02:39,360
+أمراض أو ما بتسبب أمراض كلها بتتعامل على أنها هي
+
+795
+01:02:39,360 --> 01:02:41,960
+pathogenic إيش يعني أتعامل .. أعملها على أنها
+
+796
+01:02:41,960 --> 01:02:46,120
+pathogenic؟ على أنها ممرضة تمام؟ يعني ممنوع أنا
+
+797
+01:02:46,120 --> 01:02:49,620
+خلاص أضمني هاي البكتيريا بتسببش أمراض مصرا .. خلاص
+
+798
+01:02:49,620 --> 01:02:53,820
+أ ..اشتغل فيها لأ don't trust بكتيريا ماتفقيش في
+
+799
+01:02:53,820 --> 01:02:57,500
+البكتيريا لإن هي فجأة انت ممكن تتحول من non
+
+800
+01:02:57,500 --> 01:03:02,900
+-pathogenic لpathogenic فيعني و في كتير بكتيريا
+
+801
+01:03:02,900 --> 01:03:06,460
+بنسميها البكتيريا الانتهازية اللي هي تنتهز فرصة هي
+
+802
+01:03:06,460 --> 01:03:12,690
+بتكونش ممرضة ولكن تنتهز فرصة بتصير ممرضةايضا ال
+
+803
+01:03:12,690 --> 01:03:17,650
+liquid culture اللي هي ال culture السائلة هذه لازم
+
+804
+01:03:17,650 --> 01:03:21,090
+انها تكون داخل ال rack موجودة هذا ال rack اللي هو
+
+805
+01:03:21,090 --> 01:03:24,770
+الحامل تبعها و تكون موجودة على ال bench إلى الداخل
+
+806
+01:03:24,770 --> 01:03:28,270
+ان انا لما امر مثلا ماتجعش تنكب تنكسر مني تنتشر
+
+807
+01:03:28,270 --> 01:03:32,930
+العدوى او تنتشر البكتيريا اللي في .. على الأرض و
+
+808
+01:03:32,930 --> 01:03:37,190
+ايضا مش لازم انا يعني انه المهم ما تنكسرش و تسرب
+
+809
+01:03:38,290 --> 01:03:43,690
+عندي don't engage in practical jokes or horseplay
+
+810
+01:03:43,690 --> 01:03:47,830
+in the lab يعني مثلا ما تحاولوش أنكوا تنصبوا لبعض
+
+811
+01:03:47,830 --> 01:03:54,310
+فخ داخل المختبر أو أنكوا انتوا تعملوا يعني تطلعوا
+
+812
+01:03:54,310 --> 01:03:59,930
+حس الدعابة عندكم و تبدوا تعملوا مع بعض مجالب فتحطي
+
+813
+01:03:59,930 --> 01:04:06,510
+لصحبيتك مادة غريبة ولاتخليها تشم أو انك انت مثلا
+
+814
+01:04:06,510 --> 01:04:12,350
+ترشيها عليها او تخوفيها تجيبيلها اشي غريب داخل
+
+815
+01:04:12,350 --> 01:04:18,050
+المختبر لإن ممكن اي ركضة تركض مثلا و هي باللاوعي
+
+816
+01:04:18,050 --> 01:04:23,370
+تبعها خايفة او تنكسر او ينكب عليها اشي ممكن اذا
+
+817
+01:04:23,370 --> 01:04:28,630
+انت بدلتيلها مادة بمادة اخرى يعني يلحقها اذا او
+
+818
+01:04:28,630 --> 01:04:33,070
+اذا مثلا طلع .. يعني حكيتيلهاهي ماكنتش سامة ..
+
+819
+01:04:33,070 --> 01:04:37,570
+المس حكت شمه، دوجه و هي المس ماحكتش .. يعني انت
+
+820
+01:04:37,570 --> 01:04:42,150
+بتتركني روحتيها يعني كانت عندنا في سنة من السنوات
+
+821
+01:04:42,150 --> 01:04:47,170
+صارت .. كنا بنشتغل في فحص .. في مختبر الكيميا
+
+822
+01:04:47,170 --> 01:04:53,310
+الحيوية و كانت .. كنا بنستخدم الacetic acid المركز
+
+823
+01:04:53,310 --> 01:04:59,450
+طبعا خالي يعني مركز جدا فال .. البنت حكت لزملتها
+
+824
+01:04:59,450 --> 01:05:07,450
+شمهفيعني زميلتها اشتمته فيعني قعدت فترة طويلة يعني
+
+825
+01:05:07,450 --> 01:05:12,170
+تقريبا نص ساعة اختناق عندها يعني بتسعى للبنت بشكل
+
+826
+01:05:12,170 --> 01:05:15,790
+قوي جدا و مش قادرة تشم و هو اصلا يعني لما هي شمته
+
+827
+01:05:15,790 --> 01:05:23,210
+عمل تهيج للانسجة في الداخل فيعني احنا البنت ..
+
+828
+01:05:23,270 --> 01:05:28,790
+احتجنا ان احنا مثلا نخليها تشرب مي و انها .. حطها
+
+829
+01:05:28,790 --> 01:05:33,330
+في مكان تهوية فيه كتيرة و نفكلها الشالة و يعني
+
+830
+01:05:33,330 --> 01:05:38,390
+عملنا لها إسعافات أولية لحتى نخلصها من أثر أنها هي
+
+831
+01:05:38,390 --> 01:05:43,370
+شمّة حمض قوي فبالتالي رجاء ما تمزحوا مع بعض هيك
+
+832
+01:05:43,370 --> 01:05:46,550
+مزحات جديدة، حس الدعابة برا المختبر، مش داخل
+
+833
+01:05:46,550 --> 01:05:52,140
+المختبرطبعا keep non-essential book and clothing
+
+834
+01:05:52,140 --> 01:05:54,920
+far away from your working area يعني أنت ماعندك
+
+835
+01:05:54,920 --> 01:05:58,160
+الكتب و الملازم و الأشياء مش لازم تكون موجودة على
+
+836
+01:05:58,160 --> 01:06:01,340
+نفس ال bench اللي بتشتغلي فيه لأ ممكن أنت مثلا
+
+837
+01:06:01,340 --> 01:06:06,140
+تشتغلي على ال bench أو على الأماكن المخصصة للعمل و
+
+838
+01:06:06,140 --> 01:06:09,200
+لما تطلع معاكي نتيجة على طول تشلح ال gloves تغسل
+
+839
+01:06:09,200 --> 01:06:12,540
+يديك و تروح تسجل النتيجة تبعك على دفترك هيك أكثر
+
+840
+01:06:12,540 --> 01:06:18,300
+أمانا و سلاما بحيث أن دفاترك تبقى نظيفة تبقى مش ..
+
+841
+01:06:18,480 --> 01:06:22,900
+مولوثة فبالتالي تظلي محافظة على سلامتك و سلامة أي
+
+842
+01:06:22,900 --> 01:06:27,300
+حدا ممكن يستخدم شنتك او يستخدم ي .. يلامس أغراضك
+
+843
+01:06:27,300 --> 01:06:31,220
+او يلامسك انت بشكل مباشر او يحتك فيك بشكل مباشر
+
+844
+01:06:31,220 --> 01:06:36,320
+طيب بعد ما احنا نخلص شغلنا لازم انه انا انظف ال
+
+845
+01:06:36,320 --> 01:06:42,360
+bench ب disinfectant طبعا و يفضل انه قبل و بعد
+
+846
+01:06:42,360 --> 01:06:45,880
+الشغل انظف او اعقم ال bench ب disinfectant
+
+847
+01:06:47,740 --> 01:06:50,960
+disinfectant يعني مادة معاقمة طبعا بنستخدم احنا
+
+848
+01:06:50,960 --> 01:06:56,020
+detol أحيانا نستخدم ethanol كحول ممكن bleach عشرة
+
+849
+01:06:56,020 --> 01:07:00,980
+في المية chlorine محلول فبالتالي حسب الشيء المتوفر
+
+850
+01:07:00,980 --> 01:07:07,400
+و بنستخدمه قبل و بعد لتنظيف و تعقيم البنشاتdispose
+
+851
+01:07:07,400 --> 01:07:11,560
+of waste products according to instructions بعض ال
+
+852
+01:07:11,560 --> 01:07:18,460
+.. طبعا إن أنا أتخلص بال waste يعني بالفضلات في
+
+853
+01:07:18,460 --> 01:07:23,380
+مكانها المناسب في فضلات أتخلص فيها بالسلة العادية
+
+854
+01:07:23,380 --> 01:07:28,340
+في فضلات ممكن تكون ذات إشعاع يعني مواد مشعة فأنا
+
+855
+01:07:28,340 --> 01:07:31,240
+بدأ أتخلص فيها داخل صناديق و الصناديق هذي أحكم
+
+856
+01:07:31,240 --> 01:07:35,260
+أغلاقها أو أحطها في صندوق رصاصو بعدين يعني يتم ..
+
+857
+01:07:35,260 --> 01:07:40,860
+يعني اتلفها بشكل معين، في مواد معضية أو ملوثة
+
+858
+01:07:40,860 --> 01:07:46,120
+ببكتيريا أو فيروسات أو فطريات فبالتالي لما أجي بدي
+
+859
+01:07:46,120 --> 01:07:51,440
+أتلفها بدي أحطها في كيسو الكيس هذا بده يفوت على
+
+860
+01:07:51,440 --> 01:07:55,460
+جهاز التعقيم ال autoclave و بعدين يطلع .. بعدين
+
+861
+01:07:55,460 --> 01:08:00,240
+أتخلص منه، إذا أنا ما أقدر كل الفضلات عندي أرميها
+
+862
+01:08:00,240 --> 01:08:02,580
+بنفس ال container، في عندي container بده يفوت على
+
+863
+01:08:02,580 --> 01:08:05,980
+التعقيم، في container بده يفوت على التعامل مع
+
+864
+01:08:05,980 --> 01:08:08,880
+المواد المشعة، في container بده أنا خاص بأتلفه
+
+865
+01:08:08,880 --> 01:08:13,380
+عادي يعني مع نفايات عاديةفبالتالي كل نوع فضلات
+
+866
+01:08:13,380 --> 01:08:15,600
+لازم يكون في إيه له container خاص و هذا داخل
+
+867
+01:08:15,600 --> 01:08:19,040
+المختبر، فيه مثلا أدوات حادة كنا هنشتغل فيها بكرا
+
+868
+01:08:19,040 --> 01:08:24,480
+الإبر و ال ..ال .. سرنجات مثلا بصير أحكيلك أنه
+
+869
+01:08:24,480 --> 01:08:28,260
+طبعا منكون في عندي سناديج لإطلاق الأدوات الحادة
+
+870
+01:08:28,260 --> 01:08:32,020
+فهتلاقيني أنا بعطيكم تعليمات أنه بس تخلصي شغلك
+
+871
+01:08:32,020 --> 01:08:38,300
+الإبرة بترميها بهذا ال box و الباقي الأدوات اللي
+
+872
+01:08:38,300 --> 01:08:43,320
+استخدمتيهم ترميهم بالسلة فهتلاقوني أنا خلال الشغل
+
+873
+01:08:43,320 --> 01:08:46,960
+العملي هعطيكم تعليمات كيف تطلفوا الفضلات اللي احنا
+
+874
+01:08:46,960 --> 01:08:51,360
+أو ال waste اللي بيطلع من شغلناعلشان نكون محافظين
+
+875
+01:08:51,360 --> 01:08:58,290
+على السلامة و الأمان تبعتناأي مادة أو حادث بيحدث
+
+876
+01:08:58,290 --> 01:09:01,170
+داخل .. يعني أي حادث بيحدث داخل المختبر، حادث كسر،
+
+877
+01:09:01,170 --> 01:09:06,370
+أي مادة بت .. بت .. ت .. تنكب، وعاء بينكسر، أي إشي
+
+878
+01:09:06,370 --> 01:09:10,690
+بي .. بينصلا بيلامسنا، أول إشي بدنا نسجله، في
+
+879
+01:09:10,690 --> 01:09:16,490
+عندنا دفتر للحوادث، لازم يسجل بالتاريخ وال ..
+
+880
+01:09:16,490 --> 01:09:21,910
+اليوم وإيش صار بالظبط وكيف تم معالجة الخطأ ومع
+
+881
+01:09:21,910 --> 01:09:26,520
+توقيع الناس اللي موجودة داخل المختبرتمام؟ و أيضا
+
+882
+01:09:26,520 --> 01:09:30,920
+لازم أنا علشان أتخلص من آثار هذا الحادث أسأل ال
+
+883
+01:09:30,920 --> 01:09:33,340
+supervisor تبعي أسأل ال miss اللي موجودة داخل
+
+884
+01:09:33,340 --> 01:09:36,720
+المختبر miss أنا كيف أنظف هذا ..اللي انكب او اللي
+
+885
+01:09:36,720 --> 01:09:41,480
+انكسل مثلا عندي دم انكب على ال bench هذا ال blood
+
+886
+01:09:41,480 --> 01:09:46,100
+بدي انا امسحه اول حاجة ب .. ب cotton بعدين ا ..
+
+887
+01:09:46,100 --> 01:09:51,280
+انظف اعقم مكانه بكحول فبالتالي لازم يكون في عندي
+
+888
+01:09:51,280 --> 01:09:57,490
+طرق كيف اتعامل معاها مثلا انكب بحمضعلى ال bench
+
+889
+01:09:57,490 --> 01:10:00,890
+الحمض هذا مثلا لو انا بدي أدير عليه ميه ممكن يشتعل
+
+890
+01:10:00,890 --> 01:10:04,290
+لأ بروح بحطله base عشان يعادله يعمله
+
+891
+01:10:04,290 --> 01:10:08,950
+neutralization بعدين ب .. ب .. أنشفه و بامسح بميه
+
+892
+01:10:08,950 --> 01:10:12,410
+بعدين بامسح بمعقم وبالتالي هيك يعني في خطوات
+
+893
+01:10:12,410 --> 01:10:19,350
+للتخلص من أثار الحوادث فبالتاليلازم نسمع .. نسأل
+
+894
+01:10:19,350 --> 01:10:23,270
+المسة عنها علشان هي تحكيلنا كيف .. كيف نتعامل
+
+895
+01:10:23,270 --> 01:10:27,330
+معاها و نقلل من أثر الضرر اللي ممكن يلحق فينا أو
+
+896
+01:10:27,330 --> 01:10:32,210
+في زملائنا نتيجة هذا الحادث كسر أو .. انسكاب أو أي
+
+897
+01:10:32,210 --> 01:10:39,020
+إشيتمام، إيش أدوات اللي لازم نستخدمها لحتى نحقق ال
+
+898
+01:10:39,020 --> 01:10:42,900
+safety، الأمان؟ طبعا في عندي شاور، الشاور هذا و
+
+899
+01:10:42,900 --> 01:10:49,080
+مجرد ما أني أنا أشد الجزء هذا، طبعا هاي الجزء هاد،
+
+900
+01:10:49,080 --> 01:10:52,440
+مجرد ما أشده بتنزل مي، هذا طبعا إذا إيش انكب
+
+901
+01:10:52,440 --> 01:10:56,520
+علينا، أي .. على ال .. على إيدانا أو على أي جزء من
+
+902
+01:10:56,520 --> 01:11:01,510
+جسمنا، مادة، ممكن تكون حارقة أو ممكن تكون مؤذيةال
+
+903
+01:11:01,510 --> 01:11:07,130
+.. في عندي شوار .. شوار لل eye طبعا كمان هذا إذا
+
+904
+01:11:07,130 --> 01:11:11,710
+كان أي رذاذ داخل .. داخل العين لازم أنه نعرض العين
+
+905
+01:11:11,710 --> 01:11:17,550
+لهذا الشوارط ال fire blanket إذا احنا مثلا شخص
+
+906
+01:11:17,550 --> 01:11:22,690
+يعني لا سمح الله نكب عليه إشي مشتعل أو عم بينحرج
+
+907
+01:11:22,690 --> 01:11:28,650
+فلازم نلفه في بطانية عشان تمنع من أنه هذا النار
+
+908
+01:11:28,650 --> 01:11:33,510
+تبقى شاعلةو في عندي fire extinguisher برضه كمان
+
+909
+01:11:33,510 --> 01:11:42,970
+اللي هو الجرة الغاز اللي بتطفي ال .. النيران طبعا
+
+910
+01:11:42,970 --> 01:11:51,450
+في عندي إشارات لل hazards تمام؟ في عندي إشارات
+
+911
+01:11:51,450 --> 01:11:58,640
+للخطر علامات الخطر إيش يعني هذه الإشارات؟مثلا إذا
+
+912
+01:11:58,640 --> 01:12:02,560
+أنا شوفت .. ممكن أشوفها على أدوات أو على الخزانات
+
+913
+01:12:02,560 --> 01:12:07,620
+أو على الحائط موجودة طبعا يعني إذا أنا شوفت هذه
+
+914
+01:12:07,620 --> 01:12:10,840
+العلامة معناها المكان هذا في animal و ال animal
+
+915
+01:12:10,840 --> 01:12:14,160
+هذه ممكن تكون معضية أو ممرضة أو في .. يعني إحنا
+
+916
+01:12:14,160 --> 01:12:20,000
+بنجري عليها تجارب فمش لازم أقترب من هذا المكان هذا
+
+917
+01:12:20,000 --> 01:12:22,960
+يعني أن الأدوات اللي .. العلامة اللي بتكون موجودة
+
+918
+01:12:22,960 --> 01:12:28,420
+على هذه الأدوات أو ال ..الاماكن يعني الأدوات هذه
+
+919
+01:12:28,420 --> 01:12:33,520
+أو الأماكن موجود داخلها أدوات sharp يعني أدوات
+
+920
+01:12:33,520 --> 01:12:41,080
+حادة ممكن أنها تسبب جرؤ جروح أو أدى نظيف مثلا
+
+921
+01:12:41,080 --> 01:12:45,700
+للشخص إذا ما اتعامل معاها بطريقة صحيةheat hazard
+
+922
+01:12:45,700 --> 01:12:51,160
+الأدوات الساخنة أو الأجهزة اللي بتعطي حرارة لازم
+
+923
+01:12:51,160 --> 01:12:53,860
+يكون عليها هاي العلامة عشان انا أول ما أشوفها ان
+
+924
+01:12:53,860 --> 01:12:56,760
+انا ماألمساش بإيدي على طول مباشرة تاني أعرف ان هي
+
+925
+01:12:56,760 --> 01:13:02,370
+ممكن تعملي حرق فبالتاليهي الأشياء ساخنة لازم أنا
+
+926
+01:13:02,370 --> 01:13:06,150
+ألبس ال guante لسميكة قبل ألمسها أو أمسكها بال ..
+
+927
+01:13:06,150 --> 01:13:10,090
+بالملقة مثل ما حاكي هنا glassware hazard يعني هذا
+
+928
+01:13:10,090 --> 01:13:14,330
+المكان فيه أشياء زجاجية أنا مش لازم مثلا ال rough
+
+929
+01:13:14,330 --> 01:13:17,930
+العالي هذا موجود عليه هذه العلامة يعني أدوات
+
+930
+01:13:17,930 --> 01:13:21,990
+زجاجية مش لازم أنا أجي و أنا مش طايلة لل rough أجي
+
+931
+01:13:21,990 --> 01:13:28,190
+أحاول أن أنا أخد إشي منه ممكن يجع أداء زجاجية
+
+932
+01:13:28,190 --> 01:13:33,130
+تنكسر تأذنىchemical hazard طبعا في مواد كيميائية
+
+933
+01:13:33,130 --> 01:13:39,790
+بتكون موجودة ممكن مؤذية حارقة او بتسبب تهيج للجلد
+
+934
+01:13:39,790 --> 01:13:46,990
+او ممكن ان اجتمامها بيبقى مؤذى او فبالتالي بتسبب
+
+935
+01:13:46,990 --> 01:13:50,870
+خطر معين او مش لازم اخلطها مع بعض هالبعض فبالتالي
+
+936
+01:13:50,870 --> 01:13:53,610
+انا بتتعامل معها بحضر بيكون عليها هذه العلامة
+
+937
+01:13:53,610 --> 01:13:56,800
+chemical hazardهذه المواد أنا مش لازم لا أشمها ولا
+
+938
+01:13:56,800 --> 01:14:01,080
+ألامسها أو أتذوقها أو حتى أخلطها مع مواد أخرى بدون
+
+939
+01:14:01,080 --> 01:14:04,020
+تعليمات لازم أستنى ألمس أيش بتحكيلي كيف أتعامل مع
+
+940
+01:14:04,020 --> 01:14:07,440
+هذه المادة عشان أنا أظل بالسليم electrical hazard
+
+941
+01:14:07,440 --> 01:14:10,480
+طبعا هذه الماء اللي يعني على الأسلاك اللي بشوفها
+
+942
+01:14:10,480 --> 01:14:14,460
+عند الأجهزة الكهربائية انه هذا الجهاز من الخطر انه
+
+943
+01:14:14,460 --> 01:14:19,650
+يظل مشبوك بالكهربا فلازم أفصله بعد ما أخلصI face
+
+944
+01:14:19,650 --> 01:14:24,010
+.. I and face hazard طبعا ان المكان هذا بيتنبعث
+
+945
+01:14:24,010 --> 01:14:29,110
+منه رداد او ابخرة ممكن تكون مؤدية للعين او الوجه
+
+946
+01:14:29,110 --> 01:14:33,490
+فبالتالي لازم ان مثلا احط ال shield احط mask على
+
+947
+01:14:33,490 --> 01:14:38,650
+وجهي او اشي فبالتالي بدي انتبه لهذه العلامة
+
+948
+01:14:38,650 --> 01:14:41,830
+العلامة هذه بتكون موجودة على الأشياء القابلة
+
+949
+01:14:41,830 --> 01:14:46,110
+للاشتعال او اللي ممكن تحترقالعلامة هذه بتكون
+
+950
+01:14:46,110 --> 01:14:49,990
+موجودة على ال biohazard هتشوفوها على السلات اللي
+
+951
+01:14:49,990 --> 01:14:54,590
+بنطلف فيها الأدوات اللي لازم تتعقم قبل تطلف
+
+952
+01:14:54,590 --> 01:14:58,490
+حكيتلكم إنه في أدوات لازم أنا أعقمها قبل أطلفها
+
+953
+01:14:58,490 --> 01:15:03,850
+فهذه ال biohazard موجودة عليها يعني خطر بيولوجي
+
+954
+01:15:03,850 --> 01:15:08,210
+إيش خطر بيولوجي يعني فيروسات بكتيريا فطريات أشياء
+
+955
+01:15:08,210 --> 01:15:12,600
+معضية ممكن تكون موجودةهشوف .. هنشوفها على السلات و
+
+956
+01:15:12,600 --> 01:15:18,140
+على ال .. ال safety boxes و بعض الأدوات و بعض
+
+957
+01:15:18,140 --> 01:15:22,540
+الأكياس هورجيكم إياها خليكم تاخدوا انتباهكم منها
+
+958
+01:15:22,540 --> 01:15:27,100
+laser radiation طبعا يعني مثلا هذه يعني أن المواد
+
+959
+01:15:27,100 --> 01:15:31,700
+مشعة و أنا مش لازم أقترب أو ألامسها هيك ليش؟ لأنه
+
+960
+01:15:31,700 --> 01:15:37,510
+انا إذا بدي أقترب من هايال .. المادة يعني أنا أخدت
+
+961
+01:15:37,510 --> 01:15:40,870
+إشعاع بإيدي روحت لمست كتبي، الكتبي صارت مشععة،
+
+962
+01:15:40,870 --> 01:15:44,090
+لمست زميلتي، زميلتي صار في عندها إشعاع، كذا، و
+
+963
+01:15:44,090 --> 01:15:48,750
+الإشعاعات carcinogen مسببة لل cancer فبالتالي
+
+964
+01:15:48,750 --> 01:15:55,630
+مشكلة أو .. وكمان ممكن تعملي abnormal hormonal
+
+965
+01:15:55,630 --> 01:15:59,170
+profile، إيش يعني؟ يعني بتخرب كل شغل ال .. ال
+
+966
+01:15:59,170 --> 01:16:04,050
+hormonات داخل الجسمفبالتالي لازم انا انتبه لهاي
+
+967
+01:16:04,050 --> 01:16:08,070
+المواد المشعة حكينا ان تلفها حسب التعليمات في طرق
+
+968
+01:16:08,070 --> 01:16:13,770
+معينة الأداة هذه يعني برضه كمان ان في عندى إشعاع
+
+969
+01:16:13,770 --> 01:16:17,950
+هذا إشعاع laser وهو هذي إشعاع من أنواع أخرى أيضا
+
+970
+01:16:17,950 --> 01:16:22,430
+برضه كمان يعني مواد مشعة وخاطرة explosive يعني هذه
+
+971
+01:16:22,430 --> 01:16:25,590
+المادة قابلة للانفجار مش لازم انه انا مثلا اتركها
+
+972
+01:16:25,590 --> 01:16:29,490
+مفتوحةمش لازم ا .. ا .. احطها في .. ا .. اضل ارج
+
+973
+01:16:29,490 --> 01:16:33,090
+فيها مش لازم ا .. ا .. اضيف عليها مواد اتساعد من
+
+974
+01:16:33,090 --> 01:16:37,230
+انفجارها فبالتالي بدي اتعامل مع كل المواد هذه
+
+975
+01:16:37,230 --> 01:16:41,430
+تتعامل طبقا للتعليمات كيف يعني طبقا للتعليمات؟
+
+976
+01:16:41,430 --> 01:16:44,470
+يعني ال master بتحكيلكوا اضيفوا بالأول كذا بعدين
+
+977
+01:16:44,470 --> 01:16:49,050
+كذا طبعا انا ليش حاكتلك اضيفي بالأول ال .. المادة
+
+978
+01:16:49,050 --> 01:16:53,160
+الفلانية عشان لو انت عكستي ممكن يصير في مشكلةطبعا
+
+979
+01:16:53,160 --> 01:16:56,840
+فبالتالي بتنتبهوا لتعليمات لمست كتير منيحة و احنا
+
+980
+01:16:56,840 --> 01:17:01,620
+طبعا يعني الاشي مابناش ياكم تخافوا كتير لأ احنا
+
+981
+01:17:01,620 --> 01:17:05,880
+بنكون منتبهين كتير معاكم و مركزين و بنمشي خطوة
+
+982
+01:17:05,880 --> 01:17:09,020
+بخطوة و بالعكس أنتوا بتكونوا يعني موجودين على ال
+
+983
+01:17:09,020 --> 01:17:14,180
+bench حواليا انا بكون اول اشي بشتغل التجربة ب ..
+
+984
+01:17:14,180 --> 01:17:18,800
+بإيدي بورجيكم كيف التجربة بتنشغل بتشوفوها لأول مرة
+
+985
+01:17:18,800 --> 01:17:24,010
+بإيديكم .. بعينيكم sorryو بعد ما تشوفوا ال ..
+
+986
+01:17:24,010 --> 01:17:31,110
+الاشي بتروحوا تشتغلوا بإيداكم بعدين طبعا و أنتوا
+
+987
+01:17:31,110 --> 01:17:34,890
+بتشتغلوا أنا بكون متبعة معاكم كمان يعني ما بسيبكم
+
+988
+01:17:34,890 --> 01:17:37,790
+تشتغلوا هيك لحالكم لأ يعني أنتوا بتكونوا شوفتي
+
+989
+01:17:37,790 --> 01:17:41,250
+التجربة لأول مرة و أنا بشتغلها بعد ما أكون أنا
+
+990
+01:17:41,250 --> 01:17:45,930
+شرحتهايعني بتكون التجربة مرت عليكي للمرة التانية و
+
+991
+01:17:45,930 --> 01:17:49,410
+بعدين لما تيجي تشتغليها بإيداكي أنا بمشي معاكم
+
+992
+01:17:49,410 --> 01:17:52,810
+خطوة خطوة بقولكم يلا خطوة رقم واحد ضيفي المادة
+
+993
+01:17:52,810 --> 01:17:56,010
+كذا، خطوة رقم اتنين ضيفي المادة كذا و طبعا
+
+994
+01:17:56,010 --> 01:17:59,490
+مابننطقلش للخطوة رقم اتنين إلا لما يكون الكل أضاف
+
+995
+01:17:59,490 --> 01:18:02,710
+و الكل اشتغل و الكل ماشي علشان في النهاية نكون
+
+996
+01:18:02,710 --> 01:18:07,590
+كلنا ماشيين زي بعض و كلنا بنشتغل مع بعضو الكل أخد
+
+997
+01:18:07,590 --> 01:18:10,910
+.. منتبه و ماخد التعليمات أول بأول لكن لو تركت
+
+998
+01:18:10,910 --> 01:18:14,410
+مثلا ناس خلص و ناس لسه بدين و ناس نسلم من نص الشغل
+
+999
+01:18:14,410 --> 01:18:19,050
+هيصير في فوضى، هيصير في ناس مش صامعة تعليمات كويس،
+
+1000
+01:18:19,050 --> 01:18:22,730
+في ناس ملتزماتش كويس فلأ خطوة بخطوة و مع بعض و
+
+1001
+01:18:22,730 --> 01:18:27,230
+متابعين أول بأول علشان في النهاية تكونوا بتشتغلوا
+
+1002
+01:18:27,230 --> 01:18:31,730
+صح و بتتعلموا صح و مركزين معايا لكل كلمة بحكيها،
+
+1003
+01:18:31,730 --> 01:18:38,450
+كل كلمة بحكيها بتكون مهمةفي عندي أشياء أخرى من ال
+
+1004
+01:18:38,450 --> 01:18:47,090
+.. الرموز هي inflammable يعني مادة قابلة للاشتعال
+
+1005
+01:18:47,090 --> 01:18:52,150
+تمام هذه مادة بتسبب تهيج للجسم مادة حارقة بتسبب
+
+1006
+01:18:52,150 --> 01:18:55,610
+حروق ال bio hazard عرفنا إيش يعني ال explosion
+
+1007
+01:18:55,610 --> 01:19:00,870
+قابلة للانفجار يعرفنا إيش يعني ال .. هذه مادة سامة
+
+1008
+01:19:00,870 --> 01:19:06,580
+هذه مادة قابلة للأكسدة والاشتعالو هذه طبعا المادة
+
+1009
+01:19:06,580 --> 01:19:10,640
+ده إذا أنا لازم أتلفها بطريقة معينة مش لازم أوصي
+
+1010
+01:19:10,640 --> 01:19:15,280
+.. يعني أكبها كب بالحوض أو بالسلة لإنها لو وصلت
+
+1011
+01:19:15,280 --> 01:19:19,500
+للطربة أو للنباتات أو للبحار لل .. ممكن تأذي
+
+1012
+01:19:19,500 --> 01:19:23,200
+النباتات أو تأذي ثروة السمكية أو تأذي الحيوانات
+
+1013
+01:19:23,200 --> 01:19:28,600
+فبالتالي بتسبب خطر على البيئة تعمل تلوث بيئي فإذا
+
+1014
+01:19:28,600 --> 01:19:32,000
+وجدت هذه المادة .. العلامة على أي مادة فأنا لازم
+
+1015
+01:19:32,000 --> 01:19:36,380
+نتلفها بطريقة صحيحةو هذه معناها مادة مشعة و حكينا
+
+1016
+01:19:36,380 --> 01:19:41,580
+إيش يعني، هيك بنكون خلصنا مختصر ال lab safety و
+
+1017
+01:19:41,580 --> 01:19:44,620
+عرفنا إيش يعني ال medical laboratory، هذا عنوان
+
+1018
+01:19:44,620 --> 01:19:52,250
+محاضرتناال .. المادة .. النظرية كان و راح ان شاء
+
+1019
+01:19:52,250 --> 01:19:57,170
+الله نحكي عن ال lab safety أيضا في العمل بمعلومات
+
+1020
+01:19:57,170 --> 01:20:01,730
+أخرى تمام؟ هتشوفوا فيها تكرار تمام؟ ولكن هنضيف بعض
+
+1021
+01:20:01,730 --> 01:20:05,050
+المعلومات الجديدة و ان شاء الله تكون بسيطة وسهلة
+
+1022
+01:20:05,050 --> 01:20:08,150
+عليكم يعطيكم ألف عافية و شكرا لكم
+
diff --git a/PL9fwy3NUQKwY9jl8xw-bWkVZkWcGGyspd/QS6fztFTb_Y_raw.srt b/PL9fwy3NUQKwY9jl8xw-bWkVZkWcGGyspd/QS6fztFTb_Y_raw.srt
new file mode 100644
index 0000000000000000000000000000000000000000..12c2a336a3f6a9f408e2d0b4701bd7b567102743
--- /dev/null
+++ b/PL9fwy3NUQKwY9jl8xw-bWkVZkWcGGyspd/QS6fztFTb_Y_raw.srt
@@ -0,0 +1,4088 @@
+1
+00:00:05,720 --> 00:00:09,280
+السلام عليكم ورحمة الله وبركاته اليوم ان شاء الله
+
+2
+00:00:09,280 --> 00:00:14,000
+رح ناخد أول محاضرة نظرية في موضوع المقدمة في
+
+3
+00:00:14,000 --> 00:00:18,300
+التحليل الطبية أو مقدمة في المختبرات الطبية ان شاء
+
+4
+00:00:18,300 --> 00:00:21,380
+الله اليوم موضوعنا عن ال medical laboratories رح
+
+5
+00:00:21,380 --> 00:00:25,560
+نعرف إيش يعني مختبر طبي، إيش رسالة المختبر الطبي
+
+6
+00:00:25,560 --> 00:00:30,240
+أو الهدف الذي يجب أن يحققه المختبر الطبي، وإيش
+
+7
+00:00:30,240 --> 00:00:33,520
+طبيعة الأعمال اللي بيقوم فيها وأنواع المختبرات
+
+8
+00:00:33,520 --> 00:00:38,350
+الطبية بإذن اللهولكن في البداية يجب أن نعرج على
+
+9
+00:00:38,350 --> 00:00:44,630
+مصطلح اسمه health care provider مزودو الصحة أو
+
+10
+00:00:44,630 --> 00:00:49,310
+العناية الصحية إيش يعني مزودو العناية الصحية؟ هذا
+
+11
+00:00:49,310 --> 00:00:54,530
+المصطلح بيندرج تحت .. تحته any individual أي شخص،
+
+12
+00:00:54,530 --> 00:01:00,750
+أي مؤسسة، أي وكالة، أجنسي هي من شأنها أنها تزودأو
+
+13
+00:01:00,750 --> 00:01:05,910
+تقدم health care أو health services لشخص
+
+14
+00:01:05,910 --> 00:01:09,310
+المستفيدين من الخدمات الصحية يعني health care
+
+15
+00:01:09,310 --> 00:01:14,310
+provider مزودية الرعاية الصحية هو مصطلح بيشمل أي
+
+16
+00:01:14,310 --> 00:01:19,510
+شخص، أي مؤسسة، أي وكالة بتقدم رعاية صحية أو خدمات
+
+17
+00:01:19,510 --> 00:01:24,010
+صحية للناس اللي يعتبروا مستهلكين لهذه الخدمات
+
+18
+00:01:24,010 --> 00:01:32,280
+الصحية اللي هم بتعريف آخر المرضىوبيندرج تحت هاي
+
+19
+00:01:32,280 --> 00:01:36,500
+الأشخاص ممكن يكونوا ال physicians، الأطباء، ال
+
+20
+00:01:36,500 --> 00:01:42,020
+dentist، أطباء الأسنان، الممرضين، الأم الأسز اللي
+
+21
+00:01:42,020 --> 00:01:46,740
+هم احنا ال medical laboratory scientist تمام
+
+22
+00:01:46,740 --> 00:01:50,580
+medical laboratory scientist اللي هم احنا تمام ال
+
+23
+00:01:50,580 --> 00:01:56,890
+mental health counselor اللي هم يعنيالمزودي أو
+
+24
+00:01:56,890 --> 00:02:00,750
+يعني الاستشاريين حول الصحة النفسية والصحة العقلية
+
+25
+00:02:00,750 --> 00:02:04,910
+ال nursing التمريد وخدمات أخرى يعني زي التصوير
+
+26
+00:02:04,910 --> 00:02:11,650
+التدبي و الحكمة و التفارعات الأخرى لخدمات الصحية
+
+27
+00:02:11,650 --> 00:02:15,470
+كلهم هدول بيندرجوا تحت مصطلح health care provider
+
+28
+00:02:16,090 --> 00:02:20,550
+في عصر التكنولوجيا الحديثة تمام صار محت .. صرنا
+
+29
+00:02:20,550 --> 00:02:23,550
+محتاجين لحتى نزود او نعمل health care .. health
+
+30
+00:02:23,550 --> 00:02:29,070
+care delivery نزود او نقدم خدمات صحية لازم ان يكون
+
+31
+00:02:29,070 --> 00:02:32,970
+في عندي كتير او so many different personnel و
+
+32
+00:02:32,970 --> 00:02:36,530
+specialists ايش يعني الكلام هذا؟ زمان كان بس في
+
+33
+00:02:36,530 --> 00:02:41,120
+طبيب و في حكيم و خلاص يعنيما .. ماكنش فيه لا
+
+34
+00:02:41,120 --> 00:02:46,480
+صيدلاني ولا كان في مختبر ولا تبع تصوير طب دي، هاي
+
+35
+00:02:46,480 --> 00:02:51,200
+التخصصات كلها نشأت جديد مع تقدم التكنولوجيا، صرنا
+
+36
+00:02:51,200 --> 00:02:55,200
+محتاجين many personnel، يعني أشخاص كتير، موظفين
+
+37
+00:02:55,200 --> 00:03:00,360
+كتير، بتخصصات مختلفة، حتى كمان صرنا محتاجين التخص
+
+38
+00:03:00,360 --> 00:03:03,520
+.. في ال .. في المؤسسات اللي بتقدم رعاية صحية،
+
+39
+00:03:03,520 --> 00:03:06,720
+صرنا محتاجين تخصصات ما قالها علاقة، يعني ال .. ال
+
+40
+00:03:06,720 --> 00:03:10,590
+.. ال medicine مش هو ال background تبعهازي مثلا ال
+
+41
+00:03:10,590 --> 00:03:13,870
+Secretariat التدبية و الإدارة الصحية و الإدارة
+
+42
+00:03:13,870 --> 00:03:17,150
+التدبية، هاي كلها كانت .. يعني اللي هم علاقة
+
+43
+00:03:17,150 --> 00:03:22,250
+بأرشفة الملفات و حفظ الملفات و استخراجها و نماذج
+
+44
+00:03:22,250 --> 00:03:28,450
+ال .. الحفظ نماذج التشخيص و نماذج طلب الفحوصات،
+
+45
+00:03:28,450 --> 00:03:32,290
+هاي .. هاي عملهم تمام؟ ولكن برضه كمان زرنا
+
+46
+00:03:32,290 --> 00:03:38,050
+محتاجينهم في ال .. منظومة التقديم الرعاية الصحية
+
+47
+00:03:38,820 --> 00:03:42,560
+إذن مع تقدم التكنولوجيا صيرنا محتاجين so many
+
+48
+00:03:42,560 --> 00:03:45,320
+different personnel and specialists صيرنا بنعرف
+
+49
+00:03:45,320 --> 00:03:50,140
+إيش يعني هاي الجملة تمام ال caregivers الناس اللي
+
+50
+00:03:50,140 --> 00:03:55,600
+بتعطيني رعاية صحية إيش لازم يتوفر فيها شروط أول
+
+51
+00:03:55,600 --> 00:03:58,880
+حاجة لازم يكون عندها understanding and working
+
+52
+00:03:58,880 --> 00:04:02,740
+knowledge يعني عندها معرفة عندها background خلفية
+
+53
+00:04:02,740 --> 00:04:09,510
+بإمكانها إنها هياش تعمل تقدم diagnostic evaluation
+
+54
+00:04:09,510 --> 00:04:14,790
+يعني تقيم وضع المريض وتقييم تشخيصي لحتى تقدم رعاية
+
+55
+00:04:14,790 --> 00:04:18,850
+صحية صح تمام؟ ومن ضمن هدول الناس اللي هم ال
+
+56
+00:04:18,850 --> 00:04:21,530
+caregivers او ال care provider health care
+
+57
+00:04:21,530 --> 00:04:24,680
+providerاللي هم احنا حكينا ال medical laboratory
+
+58
+00:04:24,680 --> 00:04:29,640
+scientist فبالتالي ال laboratory و ال diagnostic
+
+59
+00:04:29,640 --> 00:04:33,460
+test تبعته المختبر و الفحوصات التشخيصية اللي بتقام
+
+60
+00:04:33,460 --> 00:04:38,140
+او بتجري داخل المختبر هي عبارة عن أداة مهمة جدا لل
+
+61
+00:04:38,140 --> 00:04:44,060
+health care delivery لتقديم الرعاية الصحيةولو انها
+
+62
+00:04:44,060 --> 00:04:46,540
+.. انها هي هذه الخدمات اللي احنا بنقدمها not
+
+63
+00:04:46,540 --> 00:04:49,460
+therapeutic، اش يعني not therapeutic؟ يعني أنا بس
+
+64
+00:04:49,460 --> 00:04:52,740
+.. بس أجرف حصات طبية، انا فعليا ما بكون ساهمت في
+
+65
+00:04:52,740 --> 00:04:56,660
+علاج المريض بشكل direct ولكن انا بساهم في علاج
+
+66
+00:04:56,660 --> 00:05:01,070
+المريض indirect، كيف هذا الاشي بيصير؟من خلال أنه
+
+67
+00:05:01,070 --> 00:05:05,350
+مثلا المريض الطبيب بيكون عنده ال .. ال history،
+
+68
+00:05:05,350 --> 00:05:09,910
+التاريخ أو ال .. ال .. الصورة العامة عن ال ..
+
+69
+00:05:09,910 --> 00:05:12,930
+التاريخ ال .. ال .. ال patient، هل عنده أمراض
+
+70
+00:05:12,930 --> 00:05:16,650
+ورثية، هل هو شكا من أمراض أو من أعراض سابقة، يعني
+
+71
+00:05:16,650 --> 00:05:20,630
+صورة .. صورة المريض أو ال .. ال .. الملف ال .. ال
+
+72
+00:05:20,630 --> 00:05:25,960
+history تبعه، تمام؟ السيرة تبعته، المرضيةو بيكون
+
+73
+00:05:25,960 --> 00:05:29,040
+أيضا كمان عند الطبيب physical examination اش يعني
+
+74
+00:05:29,040 --> 00:05:33,600
+physical examination يعني عملله .. عملله تشخيص شاف
+
+75
+00:05:33,600 --> 00:05:39,540
+الأعراض و ال .. ال .. علامات اللي ظاهرة على المريض
+
+76
+00:05:39,540 --> 00:05:44,100
+عملله فحص و شاف اش وضع المريض من الشكل الخارجي
+
+77
+00:05:44,100 --> 00:05:51,240
+تمام؟ و احنا كMLS بنقدم خدمات صحينا therapeutic بس
+
+78
+00:05:51,240 --> 00:05:55,790
+يعني بت .. بت ..كد بتساعد أنه الطبيب يتأكد to
+
+79
+00:05:55,790 --> 00:06:00,650
+confirm diagnosis تمام و من خلال أن أنا أقدم value
+
+80
+00:06:00,650 --> 00:06:03,730
+بال information معلومات قيمة عن وضع المريض
+
+81
+00:06:03,730 --> 00:06:09,210
+فبالتالي الطبيب بيقدر أنه يشخص صح فبيوصف علاج صح و
+
+82
+00:06:09,210 --> 00:06:13,610
+بالتالي بيصير في عنده response لل therapy المريض
+
+83
+00:06:13,610 --> 00:06:18,770
+راح تتحسن حالته ال patient status راح تتحسن و أيضا
+
+84
+00:06:18,770 --> 00:06:23,950
+راح يصير في عنده response لل therapyال .. نلخص إيش
+
+85
+00:06:23,950 --> 00:06:26,930
+اللي .. اللي حكيناها، حكينا إنه في .. مع
+
+86
+00:06:26,930 --> 00:06:31,010
+التكنولوجيا الحديثة صيرنا محتاجين تخصصات أكثر، عدد
+
+87
+00:06:31,010 --> 00:06:35,150
+موظفين أكثر، كان زمان المنشأات الصحية بيقتصر عملها
+
+88
+00:06:35,150 --> 00:06:39,310
+على فقط ممرض وطبيب، الآن أنتوا المنشأة الصحية طبعا
+
+89
+00:06:39,310 --> 00:06:42,690
+عندكم مثلا ميثال مستشفى الشفاء، عدد مهول من
+
+90
+00:06:42,690 --> 00:06:48,670
+الموظفينsecretaria، إدارة صحية، إدارة طبية، أرشفة
+
+91
+00:06:48,670 --> 00:06:56,670
+ملفات، حتى موظفين للإشراف على الجودة وضمان لصحة
+
+92
+00:06:56,670 --> 00:07:01,530
+العمل وجودة العمل، عندك أطباء، أخصائي مختبرات
+
+93
+00:07:01,530 --> 00:07:06,250
+طبية، صيادلة، إذن عدد كبير من ال personnel و ال
+
+94
+00:07:06,250 --> 00:07:11,670
+specialists التخصصات أيضاوحكينا إنه ال .. الشخص
+
+95
+00:07:11,670 --> 00:07:14,530
+اللي بيقدم الرعاية الصحية لازم يكون في عنده
+
+96
+00:07:14,530 --> 00:07:17,410
+understanding .. understanding and working
+
+97
+00:07:17,410 --> 00:07:21,410
+knowledge يعني لازم يكون في عنده خلفية ويفهم للوضع
+
+98
+00:07:21,410 --> 00:07:28,710
+الصحي لل ..المريض او الحالة الطبية اللى بتيجي
+
+99
+00:07:28,710 --> 00:07:32,410
+لعنده علشان يساعد في حاجة اسمها diagnostic
+
+100
+00:07:32,410 --> 00:07:36,290
+evaluation اللى هو يعني التقييم التشخيصى الصحى
+
+101
+00:07:36,290 --> 00:07:41,930
+وحكينا ايضا ان المختبرات بغض النظر عن انه هي ما
+
+102
+00:07:41,930 --> 00:07:46,310
+بتقدم therapeutic approach يعني ما بتعطيش بتساهمش
+
+103
+00:07:46,310 --> 00:07:51,030
+مباشرة في العلاج ولكن بتساهم بطريقة غير مباشرة
+
+104
+00:07:51,030 --> 00:07:56,100
+للعلاجكيف بتساهم بطريقة غير مباشرة في العلاج بإنها
+
+105
+00:07:56,100 --> 00:08:00,960
+تعملي diagnostic test بتساهق بتساعد الطبيب على إنه
+
+106
+00:08:00,960 --> 00:08:05,380
+يشخق يأكد ال diagnosis تبعه من خلال كيف يعني أكد
+
+107
+00:08:05,380 --> 00:08:09,700
+التشخيص تبعه من خلال رابط هذا النتائج الفحوصات
+
+108
+00:08:09,700 --> 00:08:14,360
+تبعتي مع ال history تبع المريض الملف المريض و
+
+109
+00:08:14,360 --> 00:08:18,940
+السيرة المرضية تبعتهمع الأعراض والعلامات الظاهرة
+
+110
+00:08:18,940 --> 00:08:22,040
+على المريض اللي ظهرت من خلال الفحص ال physically
+
+111
+00:08:22,040 --> 00:08:26,000
+وبالتالي بيصير في عندنا معلومات قيمة عن وضع المريض
+
+112
+00:08:26,000 --> 00:08:32,440
+ومدى استجابته للعلاج إذا فحوصات أجريت بدقة نتائج
+
+113
+00:08:32,440 --> 00:08:37,700
+صحيحة تشخيص صحيح وبالتالي علاج فعال هذه هي السلسلة
+
+114
+00:08:37,700 --> 00:08:42,000
+اللي بيقوم بتمر فيها مرحلة ال health care
+
+115
+00:08:42,000 --> 00:08:47,450
+providingتمام؟ و أيضا احنا كمختبرات مش بس بنساعد
+
+116
+00:08:47,450 --> 00:08:51,750
+ال .. ال .. الاطباء على انهم هم يعملوا confirm
+
+117
+00:08:51,750 --> 00:08:57,110
+diagnosis لأ احنا ايضا بنساعد في ال epidemiology
+
+118
+00:08:57,110 --> 00:09:01,930
+اش يعني ال epidemiology؟ يعني مدى التقصي لمدى
+
+119
+00:09:01,930 --> 00:09:06,130
+انتشار الوباء فعليا انتوا شايفين مثلا حاليا احنا
+
+120
+00:09:06,130 --> 00:09:11,980
+في .. في ظل جائحة جائحة coronavirusمين اللي اللي
+
+121
+00:09:11,980 --> 00:09:15,960
+على قدم و ساق و من خلالهم بنعرف عدد العينات اللي
+
+122
+00:09:15,960 --> 00:09:20,120
+تنفحصها و ال positive result كام عددهم و ال
+
+123
+00:09:20,120 --> 00:09:24,340
+negative result و مين هذا كله من خلال ال lab work
+
+124
+00:09:24,340 --> 00:09:30,080
+بنعرفه فبالتالي احنا بنساهم من خلال المساعدة او
+
+125
+00:09:30,080 --> 00:09:37,210
+التقصي المتعلق بالوبائيات وايضابال research
+
+126
+00:09:37,210 --> 00:09:42,510
+purposes أيضا المختبر إله دور في أغراض البحث
+
+127
+00:09:42,510 --> 00:09:46,250
+العلمى كيف يعني أغراض البحث العلمى يعني من خلال
+
+128
+00:09:46,250 --> 00:09:51,450
+الدراسات و الأبحاث تجري داخل المختبرات لما نحكي
+
+129
+00:09:51,450 --> 00:09:57,170
+مثلا والله تم اكتشاف أنه دواء أو formula معين
+
+130
+00:09:57,170 --> 00:10:04,150
+كصيغة معينة أو مادة كيميائية معينة مثلا إلها دور
+
+131
+00:10:04,150 --> 00:10:08,420
+فعال في إذابة الجلطاتتمام؟ هاي كيف احنا اكتشفناها؟
+
+132
+00:10:08,420 --> 00:10:12,680
+اكتشفناها من خلال ان احنا أجرينا عدة تحوصات او عدة
+
+133
+00:10:12,680 --> 00:10:18,000
+تجارب داخل المختبر و أكدنا هاي العلاقة فبالتالي
+
+134
+00:10:18,000 --> 00:10:22,600
+أيضا الأبحاث المحترمة والنتائج والاكتشافات العظيمة
+
+135
+00:10:22,600 --> 00:10:28,580
+تخرج من المختبر المختبر بيأكدال .. الفحوصات بيساعد
+
+136
+00:10:28,580 --> 00:10:32,420
+في تقصي الحالات الوبائية وظهور ال .. و تكتشاف ظهور
+
+137
+00:10:32,420 --> 00:10:35,940
+الأمراض الجديدة، بيساعد في إجراء الدراسات العلمية
+
+138
+00:10:35,940 --> 00:10:40,600
+و التقدم العلمي، المختبر أو أخصائي المختبر هو عين
+
+139
+00:10:40,600 --> 00:10:46,020
+الطبيب الثانيةالطبيب له عين تان عين بتشوف ال
+
+140
+00:10:46,020 --> 00:10:49,180
+physical examination اللي هي العلامات الظاهرة
+
+141
+00:10:49,180 --> 00:10:55,000
+خارجيا على المرض و طبعا هذه علامات طبعا متداخلة في
+
+142
+00:10:55,000 --> 00:10:58,020
+كل .. في معظم الأمراض كإيش يعني متداخلة في معظم
+
+143
+00:10:58,020 --> 00:11:01,700
+الأمراض؟ يعني مثلا الأعراض اللي ظاهرة على مريض
+
+144
+00:11:01,700 --> 00:11:10,610
+معين هي عبارة عن حمة ألم في العضلات والعظاميعني
+
+145
+00:11:10,610 --> 00:11:18,090
+مثلا ممكن تكون diarrhea فهذه العلامات التلاتة كتير
+
+146
+00:11:18,090 --> 00:11:22,550
+أمراض بيكون أعراضهم وجود قيق وإسهال وقلم في
+
+147
+00:11:22,550 --> 00:11:27,730
+العضلات والمفاصل أو في العظام وأيضا ارتفاع في درجة
+
+148
+00:11:27,730 --> 00:11:32,030
+الحرارة ال influenza والرشح والاتهابات أو النزلات
+
+149
+00:11:32,030 --> 00:11:37,740
+المعاوية وكتير أمراض حتى COVID-19 منهذه العلامات
+
+150
+00:11:37,740 --> 00:11:41,840
+من أعراضه، إذا المريض مش راح .. الطبيب راح .. مش
+
+151
+00:11:41,840 --> 00:11:48,980
+راح يقدر يحصر ال .. ال .. الأمراض اللي ممكن يخمنها
+
+152
+00:11:48,980 --> 00:11:54,300
+و تفسرله أو تشخصله إيش المريض عندهالا اذا احنا
+
+153
+00:11:54,300 --> 00:11:58,820
+عملنا له فحوصات وحكينا له والله انه المريض هذا
+
+154
+00:11:58,820 --> 00:12:04,560
+اللي عنده حمة وقلام في العضلات والعظام وعنده ايضا
+
+155
+00:12:04,560 --> 00:12:12,100
+دياريا مثلا هو عنده مثلا طفل معين في القمعة وجود
+
+156
+00:12:12,100 --> 00:12:15,180
+..كشفنا عن وجود طفل معين في القمعة، اذا راح يروح
+
+157
+00:12:15,180 --> 00:12:19,990
+فكرة المريضفكرة الطبيب أنه هذا المريض بعاني من
+
+158
+00:12:19,990 --> 00:12:25,150
+نازلة معاوية مش من virus ولا influenza ولا رشح ولا
+
+159
+00:12:25,150 --> 00:12:29,850
+أي إشي تاني، إذا الطبيب بعينه شاف العلامات
+
+160
+00:12:29,850 --> 00:12:34,690
+الخارجية اللي ظاهرة على المريض وإحنا بالعين
+
+161
+00:12:34,690 --> 00:12:40,810
+الثانية للطبيب شفنا العلامات الداخليةاستكشفنا داخل
+
+162
+00:12:40,810 --> 00:12:44,970
+المريض إيش فيه من خلال إن أحنا فحصنا عيناته و
+
+163
+00:12:44,970 --> 00:12:49,170
+أكدنا ال diagnosis، إذا إحنا دورنا لا يقل أهمية عن
+
+164
+00:12:49,170 --> 00:12:54,310
+الطبيب دي التشخيص، تمام؟ إحنا ساعد و ساند و عين
+
+165
+00:12:54,310 --> 00:12:58,600
+الطبيب الثانيطيب introduction to medical
+
+166
+00:12:58,600 --> 00:13:01,340
+laboratory technology ايش يعني مقدمة في التحاليل
+
+167
+00:13:01,340 --> 00:13:07,360
+الطبية؟ هو عبارة حكينا هو basic course مثاق تأسيسي
+
+168
+00:13:07,360 --> 00:13:11,420
+بيساعد الطلاب على انهم ياخدوا ال essential
+
+169
+00:13:11,420 --> 00:13:15,780
+knowledge المعرفة الأساسية و المهارات الأساسية
+
+170
+00:13:15,780 --> 00:13:20,400
+لحتى يعرفوا كيف يتصرفوا داخل المختبرات الطبية و
+
+171
+00:13:20,400 --> 00:13:25,440
+هذا الكلام طبعا احنا حكيناه في محاضرة التقديمطبعا
+
+172
+00:13:25,440 --> 00:13:30,280
+ايش اهمية المختبرات الطبية او اهمية المصاد طبعا
+
+173
+00:13:30,280 --> 00:13:37,400
+هيعلمنا اهمية او مدى اهمية الخدمات اللي بيقدمها
+
+174
+00:13:37,400 --> 00:13:42,310
+المختبر ايضا دورال medical laboratory technologist
+
+175
+00:13:42,310 --> 00:13:45,030
+انت ك medical laboratory technologist او medical
+
+176
+00:13:45,030 --> 00:13:49,010
+laboratory scientist ايش دورك بالضبط برضه كمان رح
+
+177
+00:13:49,010 --> 00:13:53,370
+تتعرفي عليه من خلال هذا المثاق كيف انا استخدم ال
+
+178
+00:13:53,370 --> 00:13:56,530
+laboratory wares ادوات المختبر ال instruments
+
+179
+00:13:56,530 --> 00:14:00,250
+اجهزة المختبر و كيف انا اقوم بعملية ال
+
+180
+00:14:00,250 --> 00:14:03,700
+sterilization technique اللي هي كيف اناأقوم
+
+181
+00:14:03,700 --> 00:14:09,180
+بعمليات التعقيم لحتى أضمن حكينا سلامتي، سلامة
+
+182
+00:14:09,180 --> 00:14:12,500
+المريض الذي يرتدي المختبر وسلامة زملائي اللي
+
+183
+00:14:12,500 --> 00:14:16,360
+بيشتغلوا معايا في نفس المكان، في نفس بيئة العمل و
+
+184
+00:14:16,360 --> 00:14:22,370
+لحتى أعمل sorry لحتى أعمل preventionand control of
+
+185
+00:14:22,370 --> 00:14:27,010
+laboratory accident طبعا بدرايتي كيف أستخدم
+
+186
+00:14:27,010 --> 00:14:30,350
+الأدوات كيف أستخدم الأجهزة كيف أعقم كيف أستخدم
+
+187
+00:14:30,350 --> 00:14:34,870
+المواد الكيميائية هيك أنا بكون يعني اتعلمت أو قطعت
+
+188
+00:14:34,870 --> 00:14:40,690
+شغط كتير كبير في كيف أمنع و أتحكم بحدوث ال .. ال
+
+189
+00:14:40,690 --> 00:14:44,090
+.. ال accidents اللي هي الحوادث المختبرة اللي ممكن
+
+190
+00:14:44,090 --> 00:14:49,610
+تكون ضارة أو ممكن تكون تقضيني أو تقضي زملائيأيضا
+
+191
+00:14:49,610 --> 00:14:53,250
+institution of quality control system كيف أنا أطبق
+
+192
+00:14:53,250 --> 00:14:59,370
+معايير الجودة و ضمان السلامة و ضمان الجودة داخل
+
+193
+00:14:59,370 --> 00:15:04,350
+المختبر لحتى أرفع مستوى ال accuracy و ال precision
+
+194
+00:15:04,630 --> 00:15:08,090
+عشان انا أكون بحصل على .. ب .. بشتغل مختار ..
+
+195
+00:15:08,090 --> 00:15:14,210
+مفحوصات و بحصل على نتائج بمستوى عالي من الدقة و
+
+196
+00:15:14,210 --> 00:15:17,690
+الصحة أيضا، هذا طبعا بضمنلي ال equality، برفع
+
+197
+00:15:17,690 --> 00:15:20,670
+مستوى ال quality طب .. طبعا انا .. طبعا اذا اشتغلت
+
+198
+00:15:20,670 --> 00:15:24,310
+بدقة عالية و صحة عالية و جودة عالية إيش بستفيد؟
+
+199
+00:15:24,310 --> 00:15:29,690
+بستفيد انه انا ب .. بكسب ثقةال consumers او ال
+
+200
+00:15:29,690 --> 00:15:33,750
+customers ثقة للمرضى اللى بيرتادوا المختبر تسمعوا
+
+201
+00:15:33,750 --> 00:15:37,290
+ان هذا المختبر نتائجه موثوقه هذا المختبر نتائجه مش
+
+202
+00:15:37,290 --> 00:15:42,610
+كتير ليش لأنه اذا .. اذا طبقت معايير ل quality
+
+203
+00:15:42,610 --> 00:15:46,890
+جيدا داخل المختبرات اذا انا ب .. بحصل على نتائج
+
+204
+00:15:46,890 --> 00:15:52,030
+accurate precise معايير جودة عاليةإذا أنا برتقي
+
+205
+00:15:52,030 --> 00:15:55,570
+بالمختبر تبعي وبخدمات المختبر تبعتي وبالتالي بكسب
+
+206
+00:15:55,570 --> 00:15:59,550
+ثقة ال .. ال .. الأشخاص اللي بيرتعدوا المختبر أو
+
+207
+00:15:59,550 --> 00:16:02,790
+بيستفيدوا من خدمات المختبر وبالتالي هيك أنا بكون
+
+208
+00:16:02,790 --> 00:16:08,550
+بحص العائد كتير منيح و أيضا بتزيد ال confidence
+
+209
+00:16:08,550 --> 00:16:13,870
+تبعتي بتزيد ثقتي بنفسي طالما أنه أنا مطبقة لمعايير
+
+210
+00:16:13,870 --> 00:16:18,770
+الجودة أو يعني ماشي على قوانين الجودة العالمية
+
+211
+00:16:20,600 --> 00:16:24,080
+اش يعني تعريف مختبر laboratory؟ laboratory هو
+
+212
+00:16:24,080 --> 00:16:27,640
+عبارة عن facility لما أحكي عن facility يعني منشأة
+
+213
+00:16:27,640 --> 00:16:32,800
+تمام؟ المختبر هو عبارة عن منشأة that provides
+
+214
+00:16:32,800 --> 00:16:39,540
+controlled condition تزودنا بظروف محكمة و مضبوطة
+
+215
+00:16:39,540 --> 00:16:45,440
+تمام؟ ليش؟من خلالها انه انا بقدر اشتغل scientific
+
+216
+00:16:45,440 --> 00:16:49,320
+and technological research من خلال هذه الظروف
+
+217
+00:16:49,320 --> 00:16:56,240
+المحكمة و المضبوطة انا بقدر اشتغل ابحاث علمية بقدر
+
+218
+00:16:56,240 --> 00:17:01,280
+اجري تجارب experiments and measurements و قياسات
+
+219
+00:17:01,280 --> 00:17:07,240
+تمام ايش يعني ظروف مضبطة او مضبوطة يعني controlled
+
+220
+00:17:07,240 --> 00:17:12,630
+conditionو الله انا لازم افح .. لازم اخزن ال
+
+221
+00:17:12,630 --> 00:17:16,970
+reagents تبعتي و المواد الكيميائية على درجة حرارة
+
+222
+00:17:16,970 --> 00:17:23,890
+مثلا من سفر لاربعة، اذا لازم انا اتأكد انه كل يوم
+
+223
+00:17:23,890 --> 00:17:28,590
+اجي اشيك على درجة حرارة التلاجة انها هي ده يعني
+
+224
+00:17:28,590 --> 00:17:33,520
+درجة حرارتها مضبوطةتمام؟ أنا لازم عشان أنمي
+
+225
+00:17:33,520 --> 00:17:37,480
+بكتيريا و أتعرف عليها و أشخص ال .. نوع البكتيريا
+
+226
+00:17:37,480 --> 00:17:42,580
+اللي عاملة مرض لمريد معين لازم أنمي هذه البكتيريا
+
+227
+00:17:42,580 --> 00:17:47,140
+على درجة حرارة 37 إذا جهاز ال incubator الحاضنة
+
+228
+00:17:47,140 --> 00:17:50,120
+اللي أنا بدي أنمي فيه البكتيريا هذه كل يوم بدي
+
+229
+00:17:50,120 --> 00:17:54,870
+أشيك عليه و أتأكد أنه درجة حرارته 37إذا أنا بدي
+
+230
+00:17:54,870 --> 00:17:59,850
+أشتغل بدي أفصل عينات مثلا بدي أفصلها على جهاز
+
+231
+00:17:59,850 --> 00:18:07,650
+الجهاز هذا طبعا إله عدد لفات معينة في الدقيقة
+
+232
+00:18:07,650 --> 00:18:11,830
+الواحدة بيشتغل على الكهربا إذا لازم أشيك على طيار
+
+233
+00:18:11,830 --> 00:18:16,920
+الكهربا اللي بيجي لعنده شغله لازم يكون فيهالجهاز
+
+234
+00:18:16,920 --> 00:18:22,120
+هذا يكون موزون احقق ال balance تبعه اذا لما انا
+
+235
+00:18:22,120 --> 00:18:25,900
+اكون متأكدة ان كل الأجهزة و كل الأدوات تبعتي
+
+236
+00:18:25,900 --> 00:18:31,100
+مضبوطة اذا انا وفرت اش controlled condition ضروف
+
+237
+00:18:31,100 --> 00:18:35,360
+مضبوطة لحتى اشتغل scientific research تجارب
+
+238
+00:18:35,360 --> 00:18:39,880
+experiments و قياسات measurements و تكون كلها
+
+239
+00:18:39,880 --> 00:18:46,340
+اشمالهانتائجها تعتمد تمام؟ لكن مثلا لما تكوني
+
+240
+00:18:46,340 --> 00:18:49,940
+التلاجة مرة بتفصل مرة بترتفع طب انا مش متأكدة ان
+
+241
+00:18:49,940 --> 00:18:53,120
+ال reagent هذا او المادة الكيميائية اللي موجودة في
+
+242
+00:18:53,120 --> 00:18:57,500
+ضايلة فعالة ولا خربت درجة الحرارة مش ثابتة معناه
+
+243
+00:18:57,500 --> 00:19:01,440
+انا مش هتأكد الفحص تبعي او التجربة تبعتي اللي بدي
+
+244
+00:19:01,440 --> 00:19:04,760
+اشتغلها باستخدام المادة الكيميائية هذه هتعطيني
+
+245
+00:19:04,760 --> 00:19:09,360
+نتائج دقيقة فبالتاليهذا معنى كلمة controlled
+
+246
+00:19:09,360 --> 00:19:13,240
+condition كل شيء داخل المختبر يجب أن يكون مضبوط
+
+247
+00:19:13,240 --> 00:19:19,480
+نشكي عليه نتأكد من .. من أن هو بيشتغل في الظروف
+
+248
+00:19:19,480 --> 00:19:23,980
+اللي لازم يكون موجود عليها طبعا medical laboratory
+
+249
+00:19:23,980 --> 00:19:28,390
+طبعا laboratory highعبارة عن كلمة أو مصطلح عام
+
+250
+00:19:28,390 --> 00:19:32,210
+medical laboratory هو مصطلح خاص و نفس المعنى
+
+251
+00:19:32,210 --> 00:19:37,310
+clinical laboratory مختبر طبي او مختبر سريري هو
+
+252
+00:19:37,310 --> 00:19:41,330
+عبارة عن المختبر او المنشأة تمام المختبر او
+
+253
+00:19:41,330 --> 00:19:46,330
+المنشأة اللي من خلالها انا اش بشتغل اش نوعية
+
+254
+00:19:46,330 --> 00:19:49,770
+التجارب اللي بدي اشتغلها بدي اشتغلها على clinical
+
+255
+00:19:49,770 --> 00:19:54,630
+specimen تمام؟ومن خلالها انا بدي اخد نتائج get
+
+256
+00:19:54,630 --> 00:19:58,810
+information علشان تساعدني في ال diagnosis ومن ثم ل
+
+257
+00:19:58,810 --> 00:20:02,410
+treatment و ايضا ل prevention of disease امنع
+
+258
+00:20:02,410 --> 00:20:05,170
+انتشار الأمراض من خلال ان انا احقق ال diagnosis
+
+259
+00:20:05,170 --> 00:20:09,470
+صحية treatment صحية فبالتالي بحد من انتشار الأمراض
+
+260
+00:20:09,470 --> 00:20:13,580
+بهذا الشكلطيب إيش الفرق ما بين laboratory و
+
+261
+00:20:13,580 --> 00:20:16,020
+medical laboratory؟ حكينا laboratory هي الكلمة
+
+262
+00:20:16,020 --> 00:20:20,840
+العامة أو التعريف العام لمصطلح مختبر، طبعا انتوا
+
+263
+00:20:20,840 --> 00:20:28,600
+يمكن ارتدتوا او شوفتوا مبنى لمختبرات كان مكون من
+
+264
+00:20:28,600 --> 00:20:32,240
+رد التوابق، الفيزيا عندهم مختبراتالكيميا عندهم
+
+265
+00:20:32,240 --> 00:20:35,960
+مختبرات، احنا عندنا في العلوم الطبية المخبرية
+
+266
+00:20:35,960 --> 00:20:40,340
+عندنا مختبرات وحتى في علوم الأرض والبيئة عندهم
+
+267
+00:20:40,340 --> 00:20:44,160
+مختبرات ولكن طبعا كلهم نفس الاشي عندهم control
+
+268
+00:20:44,160 --> 00:20:47,360
+condition وبيشتغلوا research وبيشتغلوا experiments
+
+269
+00:20:47,360 --> 00:20:52,140
+و measurementsولكن نوعية الدراسات أو نوعية ال
+
+270
+00:20:52,140 --> 00:20:54,800
+measurements و الفحوصات و ال experiment و التجارب
+
+271
+00:20:54,800 --> 00:20:59,380
+اللي بيشتغلوها تختلف تمام؟ مثلا بالكيميا بيجيبوا
+
+272
+00:20:59,380 --> 00:21:02,260
+مادة unknown مش معروفة بيجروا عليها فحوصات
+
+273
+00:21:02,260 --> 00:21:04,940
+كيميائية معينة بيتعرفوا على مثلا ال molecular
+
+274
+00:21:04,940 --> 00:21:10,020
+weight تبعتها بيتعرفوا على خصائصها هي Redox ولا ..
+
+275
+00:21:11,550 --> 00:21:15,410
+مثلا Reductant أو Oxidant بتعرفوا عليها أنها ..
+
+276
+00:21:15,410 --> 00:21:18,810
+يعني بالنهاية بياخدوا صورة كاملة عن المادة و ممكن
+
+277
+00:21:18,810 --> 00:21:21,390
+يعرفوها أنها هذه المادة أو هذا المحلول أو هذه
+
+278
+00:21:21,390 --> 00:21:28,030
+الصيغة .. صيغتها الكيميائية تصير معروفةال .. الأرض
+
+279
+00:21:28,030 --> 00:21:33,070
+والبيئة بيجروا في خوصات و أبحاث و قياسات ولكن ممكن
+
+280
+00:21:33,070 --> 00:21:38,190
+تكون على عينات تربة عينات ماء عينات سخور يعني هيك
+
+281
+00:21:38,190 --> 00:21:41,950
+.. هيك علم الأرض والبيئة لكن احنا ال medical
+
+282
+00:21:41,950 --> 00:21:47,170
+laboratory بتخصصنا بنجري في خوصاتtests بنشتري
+
+283
+00:21:47,170 --> 00:21:51,310
+افحوصات على إيش؟ على clinical specimen إيش يعني
+
+284
+00:21:51,310 --> 00:21:56,070
+clinical specimen عينة مرضية أو سريرية يعني عينة
+
+285
+00:21:56,070 --> 00:22:00,530
+أنا أخدتها من شخص هو suspected شك فيه أنه يكون
+
+286
+00:22:00,530 --> 00:22:04,310
+مريض طبعا مش .. أنا بجزمش أنه يكون مريض لكن شك فيه
+
+287
+00:22:04,310 --> 00:22:09,270
+أنه يكون مريضتمام؟ فبالتالي أي عينة تُأخد من شخص
+
+288
+00:22:09,270 --> 00:22:13,490
+suspected أنه يكون مريض فبالتالي بسميها clinical
+
+289
+00:22:13,490 --> 00:22:18,210
+sample عينة ال .. ال urine، ال bowel، عينة الدم،
+
+290
+00:22:18,210 --> 00:22:22,270
+عينة ال .. حتى ال .. ال .. الشعر، الأضافر، هذه
+
+291
+00:22:22,270 --> 00:22:27,730
+كلها عينات سواء الجسم، الانسجة، هذه كلها عينات
+
+292
+00:22:27,730 --> 00:22:30,770
+إحنا بناخدها من ناس شكين فيهم إن هم يكونوا مرضة أو
+
+293
+00:22:30,770 --> 00:22:35,480
+لأ و الفحوصات تثبت أو تنفي مرضهم، تمام؟فبالتالي
+
+294
+00:22:35,480 --> 00:22:39,880
+هذه بيسميها .. كلها clinical samples تمام؟ إيش
+
+295
+00:22:39,880 --> 00:22:43,820
+يعني medical laboratory science؟ إيش يعني تخصصنا؟
+
+296
+00:22:43,820 --> 00:22:49,020
+تخصصنا هو إن أنا أوظف أو أستخدم ال clinical
+
+297
+00:22:49,020 --> 00:22:54,780
+laboratory علشان إيش أعمل؟ أكتشف، أشخص، أراقب و
+
+298
+00:22:54,780 --> 00:22:59,660
+أعالج المرض تمام؟detect بدي أشوف هذا الشخص مريض
+
+299
+00:22:59,660 --> 00:23:04,920
+ولا لأ وبالتالي أ .. أ .. أعمله diagnosis و أراقب
+
+300
+00:23:04,920 --> 00:23:09,320
+حالة المريض أحيانا بيجيني مريض ممكن أطلب يطلع يعني
+
+301
+00:23:09,320 --> 00:23:12,820
+الدكتور يوجهه لعندي أعمله فحص في بداية الخطة
+
+302
+00:23:12,820 --> 00:23:16,880
+العلاجية في نص الخطة العلاجية برضه كمان أجريله فحص
+
+303
+00:23:16,880 --> 00:23:20,720
+تاني عشان أشوفه اتحسن ولا ماتحسنشطبعا و في نهاية
+
+304
+00:23:20,720 --> 00:23:23,040
+الخطة العلاجية ممكن اجريله فحص هذا بسميه
+
+305
+00:23:23,040 --> 00:23:27,800
+monitoring مراقبة طبعا و أيضا علشان نقدر احنا
+
+306
+00:23:27,800 --> 00:23:33,060
+نساهم indirectly بعلاج المرض طبعا احنا حكينا انا
+
+307
+00:23:33,060 --> 00:23:37,380
+مابساهم بشكل direct ولكن بشكل indirect طبعا ايش
+
+308
+00:23:37,380 --> 00:23:39,860
+نوع العينات اللي بتيجيني في ال clinical laboratory
+
+309
+00:23:39,860 --> 00:23:43,860
+قولنا clinical samples او clinical specimen طبعا
+
+310
+00:23:43,860 --> 00:23:48,380
+clinical specimen او clinical samples نفس المعنى
+
+311
+00:23:48,380 --> 00:23:55,410
+طبعاطيب إيش يعني؟ ممكن تكون دم blood، tissue تمام،
+
+312
+00:23:55,410 --> 00:23:59,050
+body fluid سواء الجسم، ممكن تكون دم أنتجة سواء
+
+313
+00:23:59,050 --> 00:24:03,470
+الجسم، أي نوع من .. أي عينة تأخد من شخص suspected
+
+314
+00:24:03,470 --> 00:24:07,050
+أنه يكون مريض بسميها clinical samples طبعا بحللها
+
+315
+00:24:07,050 --> 00:24:11,000
+كيميائيا، بعمللها chemical analysisو examination
+
+316
+00:24:11,000 --> 00:24:14,980
+علشان أشوف والله هذا الشخص عنده foreign organism
+
+317
+00:24:14,980 --> 00:24:20,080
+عنده مثلا جسم غريب أو كائن غريب زي بكتيريا، فطريات
+
+318
+00:24:20,080 --> 00:24:24,740
+وفيليات عملاله عدوى و اللي عنده مثلا abnormalities
+
+319
+00:24:24,740 --> 00:24:30,260
+وظائف كبد وظائف كيلة وظائف القلب وظائف الأعضاء
+
+320
+00:24:30,260 --> 00:24:34,060
+كلها أشوفها طبيعية ولا abnormal normal ولا
+
+321
+00:24:34,060 --> 00:24:39,210
+abnormal تمام هذا برضه كمانمن ضمن شغلنا كأخصائي
+
+322
+00:24:39,210 --> 00:24:44,250
+مختبرات طبية، كمان المعلومات اللي احنا حكينا بت ..
+
+323
+00:24:44,250 --> 00:24:49,250
+اللي بنستخدمها بتساعد الفريق الطبي عشان ياخد
+
+324
+00:24:49,250 --> 00:24:55,690
+قرارات تبعا لهذا المريضبتساعد طبعا انا لما بقدم
+
+325
+00:24:55,690 --> 00:25:00,430
+نتائج الفحوصات الطبيب بيصير .. تبقى .. بيربط
+
+326
+00:25:00,430 --> 00:25:03,350
+النتائج تبعتي حكينا بال history تبعت المريض
+
+327
+00:25:03,350 --> 00:25:06,210
+بالعلامات وال physical examination اللي ظاهرة على
+
+328
+00:25:06,210 --> 00:25:10,690
+المريض وبالتالي بياخد قرار ان هذا المريض مشخص بمرض
+
+329
+00:25:10,690 --> 00:25:16,920
+كذاو يسرف له العلاج كذا فبالتالي و برسمله الخطة
+
+330
+00:25:16,920 --> 00:25:21,820
+العلاجية طبعته خمسة و تمانين في المية من القرارات
+
+331
+00:25:21,820 --> 00:25:26,940
+الطبية تعتمد على ايش؟ على ال .. ال .. النتائج
+
+332
+00:25:26,940 --> 00:25:31,380
+المختبر تبعي او نتائج البحثات تبعتي اذا انا العين
+
+333
+00:25:31,380 --> 00:25:35,640
+العين الطبيب الثاني اللي بتشوف بنسبة خمسة و تمانين
+
+334
+00:25:35,640 --> 00:25:40,480
+في المية اما عين الطبيب aloneبتشوف بنسبة 15%، اذا
+
+335
+00:25:40,480 --> 00:25:45,020
+لا تستهينوا بتخصصكم، حبوا بتخصصكم، حبوا الاشي اللي
+
+336
+00:25:45,020 --> 00:25:49,680
+انتوا جايين تتعلموه و يكونوا فخورين بما تصنعوا لإن
+
+337
+00:25:49,680 --> 00:25:55,080
+انتوا تخصصكم لا يستهان فيه ولا يمكن الاستغناء عنه
+
+338
+00:25:55,450 --> 00:26:02,870
+يعني حتى الأطباء والصيادلة والطمريد لما بدهم ييجوا
+
+339
+00:26:02,870 --> 00:26:06,710
+يعملوا دراسات عليا لابد من إنهم يقوموا بإجراء
+
+340
+00:26:06,710 --> 00:26:12,320
+فحوصات وتجارب داخل مختبراتنا، تمام؟فبالتالي يعني
+
+341
+00:26:12,320 --> 00:26:18,580
+احنا تخصصنا كتير مهم و هذا يعني انه لا يقل أهمية
+
+342
+00:26:18,580 --> 00:26:22,180
+عن التخصصات الأخرى انا لا اقول ان التخصصات الأخرى
+
+343
+00:26:22,180 --> 00:26:26,620
+غير مهمة هي مهمنا و هي حجر أساس و لكن أيضا تخصصنا
+
+344
+00:26:26,620 --> 00:26:32,040
+مهم جدا و يجب ان انتوا تحبوا و تفتخروا فيه و تحسوا
+
+345
+00:26:32,040 --> 00:26:38,180
+قد جداش انتوا رح يكون دوركم فعال و قيم في عملية ال
+
+346
+00:26:38,180 --> 00:26:43,400
+health care providingعمليات تزويد الخدمات الصحية
+
+347
+00:26:43,400 --> 00:26:50,740
+لمستحقيها أو مستهلكيها what are lab tests؟ إيش
+
+348
+00:26:50,740 --> 00:26:55,600
+يعني الفحوصات الطبية؟ الفحوصات الطبية هي عبارة عن
+
+349
+00:26:55,600 --> 00:26:59,060
+ال medical procedures التجارب الطبية اللي من
+
+350
+00:26:59,060 --> 00:27:02,180
+خلالها اللي بدأ تطلب الحصول على عينة clinical
+
+351
+00:27:02,180 --> 00:27:05,920
+sample أو clinical specimen اللي هي عبارة عن blood
+
+352
+00:27:05,920 --> 00:27:10,090
+أو urine أو other tissueتمام؟ إذا ال .. ال ..
+
+353
+00:27:10,090 --> 00:27:13,630
+المصطلحات بتلاحظوا إنها مرتبطة في بعضها البعض و
+
+354
+00:27:13,630 --> 00:27:17,030
+تندرج تحت بعض، يعني بنعرف إيش يعني تخصص lab، بنصير
+
+355
+00:27:17,030 --> 00:27:19,870
+نفهم إيش يعني clinical lab أو medical lab، بنصير
+
+356
+00:27:19,870 --> 00:27:23,810
+نفهم إيش يعني lab test و إيش يعني clinical sample،
+
+357
+00:27:23,810 --> 00:27:29,250
+فكل هذه يعني معلومات مترابطة مع بعضها البعضطيب إيش
+
+358
+00:27:29,250 --> 00:27:33,930
+الدكتور هيستفيد من نتائج المختبر؟ طبعا الدكتور
+
+359
+00:27:33,930 --> 00:27:41,890
+دوره إنه يلاحظ، يعرف التغيرات اللي حاصلة على صحة
+
+360
+00:27:41,890 --> 00:27:46,450
+المريض طبعا ممكن خلال أو بعد أو قبل ظهور ال
+
+361
+00:27:46,450 --> 00:27:52,270
+symptoms الأعراض من خلال نتائج المختبرأيضا بتساعد
+
+362
+00:27:52,270 --> 00:27:57,070
+نتائج المختبر أو ال laboratory test بتساعد الربيب
+
+363
+00:27:57,070 --> 00:28:02,950
+انه يعمل diagnoses يشخص المرض يرسم خطة علاجية plan
+
+364
+00:28:02,950 --> 00:28:09,670
+patient treatment و أيضا يقيم وضع المريض هل استجاب
+
+365
+00:28:09,670 --> 00:28:14,190
+هل صار في عنده response او لألل treatment و أيضا
+
+366
+00:28:14,190 --> 00:28:19,350
+يراقب وضع المريض هالحال تتسوق أو تتحسن و هذا
+
+367
+00:28:19,350 --> 00:28:25,430
+الكلام ملخص في الجملة هذا الكلام كله هذا ملخص في
+
+368
+00:28:25,430 --> 00:28:30,130
+الجملة اللي احنا حكيناها detect, diagnose, monitor
+
+369
+00:28:30,130 --> 00:28:36,170
+and treat طبعا اذا انظروا detect بيكتشف ايش
+
+370
+00:28:36,170 --> 00:28:40,670
+التغيرات اللي حدثة على صحة المريضdiagnose بيشخص بي
+
+371
+00:28:40,670 --> 00:28:45,870
+treat بيعالج و أيضا بيعمل monitoring و evaluation
+
+372
+00:28:45,870 --> 00:28:53,570
+لحالة المريض طيب نتائج المختبر او نتائج الفحوصات
+
+373
+00:28:53,570 --> 00:28:59,710
+الطبية طبعا في عدة عوامل بتأثر عليها زي ash مثلا
+
+374
+00:28:59,710 --> 00:29:05,480
+زي ال gender ال gender ال sex و ال ash تمام؟إيش
+
+375
+00:29:05,480 --> 00:29:11,180
+يعني gender يعني بختلف؟ كيف يعني ال gender بتختلف
+
+376
+00:29:11,180 --> 00:29:17,700
+نتائج الفحوصات طبعا لإله؟ مثلا إحنا كإناس معروف
+
+377
+00:29:17,700 --> 00:29:23,160
+إنه نسبة ال hemoglobin عندنا في الدم من 11 ل 13لكن
+
+378
+00:29:23,160 --> 00:29:27,820
+ال .. ال .. الدكور ال males عندهم نسبة ال
+
+379
+00:29:27,820 --> 00:29:35,760
+homoglobin تمام من اربعتاش ل سبتاش او لسبعتاش طبعا
+
+380
+00:29:35,760 --> 00:29:40,940
+هم عندهم نسبة homoglobin اعلى طب ليش؟لأنه فيه فروق
+
+381
+00:29:40,940 --> 00:29:45,380
+تبعا لل sex أو ال gender طبعا .. طب .. احنا ك ..
+
+382
+00:29:45,380 --> 00:29:50,780
+كإناث احنا كل يوم .. كل شهر عندنا مقدار loss مقدار
+
+383
+00:29:50,780 --> 00:29:54,840
+فقدار من الدم من خلال ال administration تمام؟ و
+
+384
+00:29:54,840 --> 00:29:58,960
+عندنا أيضا ال .. ال muscle mass .. الكتلة الجسمية
+
+385
+00:29:58,960 --> 00:30:03,310
+عندنا أقل منهمفبالتالي هيكون عندنا نسبة ال
+
+386
+00:30:03,310 --> 00:30:09,370
+homoglobin أقل يعني احنا لإنه بنفقد blood بشكل
+
+387
+00:30:09,370 --> 00:30:14,550
+شهري و لإنه عندي عضلات أقل فانا محتاجة homoglobin
+
+388
+00:30:14,550 --> 00:30:19,030
+او بيكون عند ال homoglobin أقل هم عندهم ال body
+
+389
+00:30:19,030 --> 00:30:25,230
+mass أكترفمحتاجين أكتر فمحتاجين هموجلوبين أكتر لإن
+
+390
+00:30:25,230 --> 00:30:28,250
+الهموجلوبين هو اللي بيحمل ال oxygen بوصله للخلايا
+
+391
+00:30:28,250 --> 00:30:32,550
+تمام؟ عشان هيك نسبة الهموجلوبين عندهم أعلى من 14 ل
+
+392
+00:30:32,550 --> 00:30:37,290
+17 لكن إحنا عندنا الهموجلوبين من 11 ل 13، ليش؟أنا
+
+393
+00:30:37,290 --> 00:30:40,870
+بيصير عندي فقدان لل blood ب .. ب .. بفقد معاه
+
+394
+00:30:40,870 --> 00:30:45,790
+homoglobin و أيضا عندي عضلات أقل فمحتاجة oxygen
+
+395
+00:30:45,790 --> 00:30:50,610
+أقل فنسبة ال homoglobin عندي هتكون أقل من مقارنة
+
+396
+00:30:50,610 --> 00:30:57,630
+بالرجل اللي من عمري، تمام؟ طيب ال age أيضا بيأثر،
+
+397
+00:30:57,630 --> 00:31:05,720
+هلأ نسبة الموليد اللي عمرهممثلا عشرين .. sorry
+
+398
+00:31:05,720 --> 00:31:10,920
+عشرين يوم، عشر أيام أو حتى بالساعات، يعني الموليد
+
+399
+00:31:10,920 --> 00:31:15,920
+الصغيرة بالعمر أو الحديثي الولادة هدول بنلاحظ أن
+
+400
+00:31:15,920 --> 00:31:20,600
+الدم عندهم بيصل خمستاشر، سبعتاشر، عشرين بالنسبالي
+
+401
+00:31:20,600 --> 00:31:24,840
+ال homoglobinلكن مجرد مثلا ما يكبر يصير عمره سنة،
+
+402
+00:31:24,840 --> 00:31:27,260
+سنتين، بيرجع على الطبيعة، بيصير اتناش، تلاتاش،
+
+403
+00:31:27,260 --> 00:31:31,900
+احداش، زينا، زي ال adult طب ليش هذه الفوارق؟ لإن
+
+404
+00:31:31,900 --> 00:31:35,540
+هم أول ما ينولدوا بيكون .. بتكون ال .. لسه عمل
+
+405
+00:31:35,540 --> 00:31:40,560
+الرئتين مش كامل فبدهم كمية هموجلبين أكتر علشان
+
+406
+00:31:40,560 --> 00:31:45,500
+تحمي ال oxygen أكترهي هذا سبب السبب التاني هم
+
+407
+00:31:45,500 --> 00:31:49,120
+بيكونوا في حالة growth burst إيش يعني growth burst
+
+408
+00:31:49,120 --> 00:31:54,520
+انفجار في النمو تمام يعني في حالة نمو هائل يعني
+
+409
+00:31:54,520 --> 00:31:58,640
+تلاحظي أن الطفل حجمه هو عمره يوم غير عن حجمه عمره
+
+410
+00:31:58,640 --> 00:32:02,680
+شهر غير عن حجمه عمره شهرين تلاتة يعني معدل النمو
+
+411
+00:32:02,680 --> 00:32:06,740
+كتير عالي فهذا أيضا محتاج oxygen delivery فمحتاجهم
+
+412
+00:32:06,740 --> 00:32:12,110
+مجلبين أكترهذا سبب التاني و السبب التالت ان ال
+
+413
+00:32:12,110 --> 00:32:16,090
+homoglobin تبعهم نوعه .. نوع ال homoglobin اللي
+
+414
+00:32:16,090 --> 00:32:21,970
+عندهم بيكون بيمسك بال oxygen كتير ف .. فكقالية
+
+415
+00:32:21,970 --> 00:32:25,030
+تعويض ربنا عوضهم على ان هو بيمسك بال oxygen مبرداش
+
+416
+00:32:25,030 --> 00:32:30,130
+يتركه عند الخلايا مبرداش يوديله الخلايا يوصلهفربنا
+
+417
+00:32:30,130 --> 00:32:34,710
+يعوضهم انه يكون نسبة ال homoglobin عالية عشان انه
+
+418
+00:32:34,710 --> 00:32:37,990
+جد ما يمسك بال oxygen يكون في نسبة .. في نسبة
+
+419
+00:32:37,990 --> 00:32:43,710
+homoglobin جادرة توصلتمام؟ إذا هاي نتائج مختبر
+
+420
+00:32:43,710 --> 00:32:48,030
+اختلفت تبعا ل ال gender و ال age طبعا what you eat
+
+421
+00:32:48,030 --> 00:32:52,130
+and what you drink أكلتي سكريات كتير بيرتفع نسبة
+
+422
+00:32:52,130 --> 00:32:55,750
+السكر في الدم أكلتي دهون كتير أو كنتي لسه ماكلة
+
+423
+00:32:55,750 --> 00:33:00,330
+وجبة و بعدين أخدنا منك عينة blood و روحنا نفحصلك
+
+424
+00:33:00,330 --> 00:33:03,810
+دهون بنلاقي الدهون عالية لإن انت لسه يدهبك ماكلة
+
+425
+00:33:03,810 --> 00:33:08,110
+وجبة في بعض الفحوصات مثلا من .. من .. منشرط على
+
+426
+00:33:08,110 --> 00:33:11,320
+الشخص أنه لازم تيجي صايملأنها بتتأثر بالأكل
+
+427
+00:33:11,320 --> 00:33:16,060
+والشرب، في بعض الفحوصات بنشرط على المريض أو بنح ..
+
+428
+00:33:16,060 --> 00:33:20,400
+يعني بنعطي تعليمات للمريض إنه نوعيات معينة من
+
+429
+00:33:20,400 --> 00:33:24,180
+الأكل ممنوعة تاكلها، يعني مثلا في ف .. في فحص ال
+
+430
+00:33:24,180 --> 00:33:28,620
+adrenaline، هرمون ال adrenaline، لما أنا بدي أعمله
+
+431
+00:33:28,620 --> 00:33:33,000
+بحكي للمريض ماتيجيش تاكل أي إشي م .. م .. محتوط
+
+432
+00:33:33,000 --> 00:33:39,620
+عليه vanilla، تمام؟أي مشتقة لكريمة و ال cake و ال
+
+433
+00:33:39,620 --> 00:33:45,400
+ice cream و أي إشي يحتوي على vanilla ما .. مايجيش
+
+434
+00:33:45,400 --> 00:33:49,860
+ماكله المريض لإنه هيأثر على الفحص لإن المادة اللي
+
+435
+00:33:49,860 --> 00:33:53,800
+بقيسها بتشبه تركيب ال vanilla يعني بتأثر عليها
+
+436
+00:33:53,800 --> 00:33:58,940
+يعني فبالتالي إش إحنا بناكل، إش إحنا بنشرب أيضا
+
+437
+00:33:58,940 --> 00:34:04,380
+بتأثر على نتائج المختبر الأدوية اللي بناخدها أيضا
+
+438
+00:34:04,380 --> 00:34:08,270
+بتأثر على المختبراحيانا مثلا لما أنا بدي أجي أشوف
+
+439
+00:34:08,270 --> 00:34:13,970
+أعمل مزرعة لمريد لازم المريض يكون مش ماخد مضادات
+
+440
+00:34:13,970 --> 00:34:19,390
+حيوية لإنه إذا هو ماخد مضادات حيوية مش راح يبين
+
+441
+00:34:19,390 --> 00:34:24,670
+عندي بكتيريا مش راح تنمو البكتيريا أو الفطريات
+
+442
+00:34:24,670 --> 00:34:28,470
+مثلا اللي مسببة ال infection مسببة العدوى عنده
+
+443
+00:34:28,470 --> 00:34:33,000
+فبالتالي نتائج المزرعة راح تفشلفبالتالي ايضا بعض
+
+444
+00:34:33,000 --> 00:34:37,500
+الأدوية يحذر او يعني ما .. ما يحبذ انهم ياخدوها
+
+445
+00:34:37,500 --> 00:34:42,660
+قبل إجراء الفحص و بتأثر نتائج الفحصات بنوع الأدوية
+
+446
+00:34:42,660 --> 00:34:47,340
+اللي بياخدها المريض قبل إجراء الفحصو أيضا جدش هو
+
+447
+00:34:47,340 --> 00:34:51,240
+التابع التعليمات طبعا في بحوصات بيجي المريض أنا
+
+448
+00:34:51,240 --> 00:34:55,620
+بشرحله التعليمات كيف هو يجي .. مثلا في .. في عينات
+
+449
+00:34:55,620 --> 00:34:58,820
+أنا بجمعها من المريض يعني أنا مثلا باخد منه عينة
+
+450
+00:34:58,820 --> 00:35:03,960
+الدم انا باخد يعني او طبيب كمان ممكن ياخد منه
+
+451
+00:35:03,960 --> 00:35:11,600
+عينات سواء الجسمو عينات الأنسجات لكن في العينات ال
+
+452
+00:35:11,600 --> 00:35:15,240
+.. المريض بنفسه بيجمعها فبالتالي أنا لازم أعطي
+
+453
+00:35:15,240 --> 00:35:18,360
+تعليمات كيف يجمعها بشكل صحيح عشان أنا أجري فحص و
+
+454
+00:35:18,360 --> 00:35:23,460
+أخد نتائج صحيحة و بالتالي تكون النتائج فعالة
+
+455
+00:35:23,880 --> 00:35:28,380
+فبالتالي أيضا جدش هو التزم و اتبع ال .. ال
+
+456
+00:35:28,380 --> 00:35:34,060
+brightest instruction التعليمات ما قبل الفحص بي ..
+
+457
+00:35:34,060 --> 00:35:38,360
+بتكون الفحص ناجح التعليمات هذه كمان مش بس بيجمع
+
+458
+00:35:38,360 --> 00:35:42,220
+العينات يعني كيف هو يجمع العينة لأ برضه كمان بال
+
+459
+00:35:42,220 --> 00:35:45,980
+.. بالمعلومات السابقة يعني مثلا ماتاخدش مضادات
+
+460
+00:35:45,980 --> 00:35:49,280
+حيوية قبل إجراء الفحص إذا هو التزم أيضا الفحص
+
+461
+00:35:49,280 --> 00:35:53,580
+هينجحما تاكلش ما تشربش تعال صايم مثلا من 6 ل 8
+
+462
+00:35:53,580 --> 00:35:58,840
+ساعات أنواع معينة من الأكل لازم تتجنبها قبل إجراء
+
+463
+00:35:58,840 --> 00:36:04,200
+الفحص في فحوصات لازم ياكل إشي معين قبل إجراءه يعني
+
+464
+00:36:04,200 --> 00:36:10,840
+مثلا أحيانا عشان نشخص مشاكل الانسولين بعطي أنا
+
+465
+00:36:10,840 --> 00:36:15,780
+محلول سكري بخليه يشربه و بجري الفحص بعد نص ساعة
+
+466
+00:36:15,780 --> 00:36:22,400
+منه و بعد ساعة و بعد ساعتين فإذاتعليمات قبل إجراء
+
+467
+00:36:22,400 --> 00:36:30,320
+الفحص مهمة جدا وعلى أساسها بتختلف أو بتتأثر نتائج
+
+468
+00:36:30,320 --> 00:36:38,020
+الفحصات طبعاال .. ال .. نتائج المختبر أو فحوصات
+
+469
+00:36:38,020 --> 00:36:44,960
+المختبر بت .. بتورجيني قداش الشخص هذا normal او
+
+470
+00:36:44,960 --> 00:36:50,000
+abnormal يعني نتائجه إذا وقعت داخل ال normal range
+
+471
+00:36:50,000 --> 00:36:54,240
+معناها هو شخص normal أما إذا وقعت النتائج خارج ال
+
+472
+00:36:54,240 --> 00:36:58,040
+normal range أقل أو أعلى فهو شخص abnormal عنده
+
+473
+00:36:58,040 --> 00:37:03,460
+مشاكل ويجب أن تعالج طبعاالنتائج المختبر مابعطيهاش
+
+474
+00:37:03,460 --> 00:37:08,660
+قيمة والله مثلا نسبة ال homoglobin الطبيعية 11 لأ
+
+475
+00:37:08,660 --> 00:37:12,060
+غلط احكي هيك نسبة ال homoglobin الطبيعية عند
+
+476
+00:37:12,060 --> 00:37:15,920
+الإينات من 11 ل 13 و عند الرجال من 14 ل 16 او من
+
+477
+00:37:15,920 --> 00:37:21,000
+14 ل 17 هذا الكلام الصحيح لإن انا ممكن يكون دمي 11
+
+478
+00:37:21,000 --> 00:37:24,800
+و ازميلتي 11.5وزميلتي التانية اتناش وزميلتي
+
+479
+00:37:24,800 --> 00:37:29,940
+التالتة تلتاش وكلنا normal فبالتالي فيه فروق فردية
+
+480
+00:37:29,940 --> 00:37:34,840
+من شخص لآخر من حسب الكتلة الجسمية تبعته حسب
+
+481
+00:37:34,840 --> 00:37:40,580
+الاستجابة للهرمونات تبعته ومعدل نموه فبالتالي هذه
+
+482
+00:37:40,580 --> 00:37:44,200
+كلها بتأثر على نتائج المختبر فانا مابقدر احكي انه
+
+483
+00:37:44,200 --> 00:37:49,880
+النتيجة الطبيعية لفحص معين قيمة بحد ذاتها لأ
+
+484
+00:37:50,290 --> 00:37:55,030
+النتيجة الطبيعية لفحص هي لازم تكون واقعة في range
+
+485
+00:37:55,030 --> 00:38:00,210
+في مدى من إلى تمام لإنه في فروق فردية فعشان هيك
+
+486
+00:38:00,210 --> 00:38:03,890
+بنراعي الفروق الفردية و بنعتبر أنه normal يقع من
+
+487
+00:38:03,890 --> 00:38:10,130
+إلى أي شخص نتيجته خارج هذا المدى اللي من إلى
+
+488
+00:38:10,130 --> 00:38:14,730
+بنعتبره abnormal سواء أقل أو أعلى هو عنده مشكلة و
+
+489
+00:38:14,730 --> 00:38:21,350
+يجب أن تعالجطيب أنواع المختبرات التدبية في عندي
+
+490
+00:38:21,350 --> 00:38:27,050
+طبعا حسب موقعها إذا كانت داخل مستشفى attached to
+
+491
+00:38:27,050 --> 00:38:30,530
+hospital متصلة بالمستشفى فبسميها hospital
+
+492
+00:38:30,530 --> 00:38:37,010
+laboratory تمام؟ و هذه بتجري بحوصات على المرضى إذا
+
+493
+00:38:37,010 --> 00:38:41,110
+كان المختبر
+
+494
+00:38:41,110 --> 00:38:47,340
+مش متصل بمستشفى فبسميه privateأو community lab
+
+495
+00:38:47,340 --> 00:38:52,380
+طبعا مختبر خاص بيستلم عينات من ال .. من السواق كان
+
+496
+00:38:52,380 --> 00:38:56,400
+من الأطباء بيواجهوهم يعني الطبيب بيحكي للمريض روح
+
+497
+00:38:56,400 --> 00:39:00,700
+على المختبر يقولاني وعملي فحص كذا المريض نفسه
+
+498
+00:39:00,700 --> 00:39:03,940
+بيروح على المختبر وممكن يجري فحوصات عشان يطمن على
+
+499
+00:39:03,940 --> 00:39:10,040
+صحته في بعض الشركاتبتبع تعيينات لهذه المختبرات
+
+500
+00:39:10,040 --> 00:39:16,000
+الخاصة بحيث إنها هي تشتغلها و تقيم .. و تشوف هذه
+
+501
+00:39:16,000 --> 00:39:20,660
+الشركات تقيم شغل المختبرات الخاصة و على أساسها هذا
+
+502
+00:39:20,660 --> 00:39:26,560
+المختبر يرتقي أو إنه بياخد لفت نظرفبنقيم ال
+
+503
+00:39:26,560 --> 00:39:30,740
+quality تبعته من خلال ال .. هذا عشان .. عشان برضه
+
+504
+00:39:30,740 --> 00:39:34,860
+كمان انا اعطي ترخيص او اجددله ترخيص لازم برضه كمان
+
+505
+00:39:34,860 --> 00:39:40,880
+انا اختبر شغله، ايضا يجرى داخل ال private labs
+
+506
+00:39:40,880 --> 00:39:50,040
+يعني أبحاث طبية ممكن برضه كمان نشتغلهاإذا في عندي
+
+507
+00:39:50,040 --> 00:39:54,700
+نوعين حسب موقعهم ال laboratory ال hospital lab و
+
+508
+00:39:54,700 --> 00:39:57,860
+ال private lab اللي هو المختبر المستشفى و المختبر
+
+509
+00:39:57,860 --> 00:40:03,400
+الخاص طبعا في حاجة اسمها تدوير عينات a lot of
+
+510
+00:40:03,400 --> 00:40:06,680
+samples are sent between different labs for
+
+511
+00:40:06,680 --> 00:40:11,000
+uncommon testsيعني مثلا احنا فاتهين مختبر خاص
+
+512
+00:40:11,000 --> 00:40:15,300
+private lab على جدنا و ماعنديش كل الأجهزة متوفرة،
+
+513
+00:40:15,300 --> 00:40:19,820
+إجها مريض، المريض هذا بده فحص مثلا growth hormone،
+
+514
+00:40:19,820 --> 00:40:24,420
+فحص هرمون النمو، و هذا أنا مابقدر أوفرله يعنىفأنا
+
+515
+00:40:24,420 --> 00:40:29,680
+ممكن أخد عينة من المريض و بعدين أبعت العينة بشكل
+
+516
+00:40:29,680 --> 00:40:33,740
+أنا أتواصل من مختبر لمختبر آخر أبعت العينة على
+
+517
+00:40:33,740 --> 00:40:39,000
+مختبر آخر هو يعني عنده أجهزة أكتر تطوران و عنده
+
+518
+00:40:39,000 --> 00:40:42,520
+فحوصات أكتر بيجدم خدمات أكتر أبعتله إياها و هو
+
+519
+00:40:42,520 --> 00:40:48,880
+يشتغلها بحيث أنه في النهاية ثمن الفحص بيتقاسموا
+
+520
+00:40:48,880 --> 00:40:54,870
+المختبرين طبعا فإذا it's more cost effectiveانه
+
+521
+00:40:54,870 --> 00:41:00,390
+يعني ال .. بي .. بيأثر على سعر الفحص يعني انه
+
+522
+00:41:00,390 --> 00:41:03,650
+بيوفر في سعر الفحص مثلا ان مختبرات مثلا تسحب
+
+523
+00:41:03,650 --> 00:41:07,670
+العينة و مختبرات بتشتغل الشغل فتالي هي انهم هيك
+
+524
+00:41:07,670 --> 00:41:14,950
+بتقاسموا العينات و ال money ما بينهمطيب أنواع
+
+525
+00:41:14,950 --> 00:41:20,670
+المختبرات أو أقسام المختبرات تبعا لتخصيصيتها يعني
+
+526
+00:41:20,670 --> 00:41:25,130
+داخل المختبر لازم يكون في عندى أقسام في أقسام
+
+527
+00:41:25,130 --> 00:41:29,330
+مختلفة طبعا ان هم ال .. ال .. ال main department
+
+528
+00:41:29,330 --> 00:41:34,170
+الأقسام الأساسية تلاتة ولكن يتفرع تحتها أشياء أخرى
+
+529
+00:41:34,170 --> 00:41:37,950
+زى مثلا في قسم لازم يكون داخل المختبر اسم clinical
+
+530
+00:41:37,950 --> 00:41:42,900
+pathology اللي هو الأمراض السريريةطبعا هذا بي ..
+
+531
+00:41:42,900 --> 00:41:46,720
+بيجرى في كل فحوصات الهيماتولوجي، كل فحوصات
+
+532
+00:41:46,720 --> 00:41:51,400
+الانسجا، كل فحوصات الأمراض الخلاية وحتى ال routine
+
+533
+00:41:51,400 --> 00:41:56,720
+pathology يعني، فحوصات ال urine و ال .. ال stool
+
+534
+00:41:56,720 --> 00:42:02,450
+أيضا فيهمفي قسم ال microbiology اش يعني قسم ال
+
+535
+00:42:02,450 --> 00:42:05,770
+microbiology يعني قسم الأحياء الدقيقة اللى بيكشف
+
+536
+00:42:05,770 --> 00:42:09,250
+عن وجود بكتيريا ال bacteriology ال virology
+
+537
+00:42:09,250 --> 00:42:14,210
+الفيروسات ال mycology الفطريات الفطريات ال
+
+538
+00:42:14,210 --> 00:42:18,370
+parasitology طفيليات وحتى بعض فحوصات المناعة و
+
+539
+00:42:18,370 --> 00:42:23,760
+الأمثال بتكون بتجرى داخل هذا القسمقسم الكيميا أو
+
+540
+00:42:23,760 --> 00:42:28,760
+الكيميا الحيوية أو الكيميا الطبية بنجري فيه فحوصات
+
+541
+00:42:28,760 --> 00:42:32,160
+الكيميا يعني وظائف الأعضاء وظائف الكيلا وظائف
+
+542
+00:42:32,160 --> 00:42:37,100
+الكبد وكذا و أيضا فحوصات السكر و الدهون و كل هاي
+
+543
+00:42:37,100 --> 00:42:41,240
+الشغلات بنسميها biochemical tests تمام؟ و أيضا
+
+544
+00:42:41,240 --> 00:42:43,900
+فحوصات الهرمونات يجري داخل هذا القسم
+
+545
+00:42:46,480 --> 00:42:53,740
+طبعا بيتفرعوا كل قسم بيتفرع إلى فروع داخل القسم
+
+546
+00:42:54,720 --> 00:42:58,660
+طبعا في عندي منشأة اسمها ال blood bank، بنك الدم
+
+547
+00:42:58,660 --> 00:43:04,980
+طبعا هذا هو جسم منفرد بنفسه separate body يعني أو
+
+548
+00:43:04,980 --> 00:43:09,340
+منشأة مش منفردة بحد ذاتها يعني ممكن أنا ألاجي بنك
+
+549
+00:43:09,340 --> 00:43:13,380
+دم مالوش علاقة لا بمختبر خاص ولا بمستشفة زي بنك
+
+550
+00:43:13,380 --> 00:43:20,660
+الدم العام هذاطبعا؟ فهو ممكن يكون متصل أو لا يكون
+
+551
+00:43:20,660 --> 00:43:26,000
+متصل بمستشفى Bank الدم، طبعا؟ وإيش وظيفته؟ وظيفته
+
+552
+00:43:26,000 --> 00:43:32,020
+إن هو بيشتغل فحوصات سواء كان .. يعني بيتأكد إن
+
+553
+00:43:32,020 --> 00:43:36,310
+الدم نظيف مافيش فيه ..infectious disease يعني الدم
+
+554
+00:43:36,310 --> 00:43:39,230
+اللي هي .. اللي .. اللي احنا بناخده من المتبرعين و
+
+555
+00:43:39,230 --> 00:43:43,470
+بدنا نصرفه لالمستقبلين أول حاجة هو يتأكد من نظافته
+
+556
+00:43:43,470 --> 00:43:46,970
+مش فيه بكتيريا ولا فيروسات مثلا زي ال hepatitis
+
+557
+00:43:46,970 --> 00:43:52,570
+انها ممكن تقضي المستقبل و أيضا من خلال بيجري
+
+558
+00:43:52,570 --> 00:43:56,570
+فحوصات على عينا الوحدات الدم اللي بناخد .. طلقاها
+
+559
+00:43:56,570 --> 00:44:01,270
+من المتبرعين بنعرف فصيلة الدم ال blood groupنسبة
+
+560
+00:44:01,270 --> 00:44:05,070
+الدم أو نسبة ال homoglobin داخل الوحدة و أيضا مدى
+
+561
+00:44:05,070 --> 00:44:10,990
+تطابق ال .. ال .. الدم هذا يعني مثلا أجاني متبرع
+
+562
+00:44:10,990 --> 00:44:14,610
+أخدت منه وحدة دم وحدة الدم هذه في ال .. في ال
+
+563
+00:44:14,610 --> 00:44:17,090
+blood bank أنا بدأ أتأكد إنها نظيفة فاش فيها
+
+564
+00:44:17,090 --> 00:44:20,690
+فيروسات أو بكتيريا ممكن تسبب أمراض مرضية للمستقبل
+
+565
+00:44:20,690 --> 00:44:25,870
+بدأ أتأكد من وحدتها و نسبة ال homoglobin فيهاولمّا
+
+566
+00:44:25,870 --> 00:44:31,510
+يجيني مستقبل محتاج وحدة الدم هذه أخد منه عينة و
+
+567
+00:44:31,510 --> 00:44:35,610
+أجرب حصاد تطابق عشان أشوف هذه العينة مطابقة للدم
+
+568
+00:44:35,610 --> 00:44:39,790
+تبعه و بالتالي لو هو أخدها مش هتظهر عليه أعراض
+
+569
+00:44:39,790 --> 00:44:45,010
+ممكن ت .. تودي بحياته أو لأتمام؟ فإذا فحوصات
+
+570
+00:44:45,010 --> 00:44:50,110
+التطابق و الفصائل الدم و أيضا الفحوصات ل ال
+
+571
+00:44:50,110 --> 00:44:53,290
+infectious disease اللي بتجرى على وحدات الدم اللي
+
+572
+00:44:53,290 --> 00:44:57,310
+بتلقاها من المتبرعين هذه كلها بتجرى داخل ال blood
+
+573
+00:44:57,310 --> 00:45:02,530
+bank و نقولنا أن هو ممكن .. ممكن يكون أو لا يكون
+
+574
+00:45:02,530 --> 00:45:06,010
+متصل بمستشفى طيب
+
+575
+00:45:07,940 --> 00:45:12,180
+أنواع المختبر طبعا حسب ال service حسب الخدمة اللي
+
+576
+00:45:12,180 --> 00:45:17,840
+بيقدموها احنا لحظوا انه حسب المكان طبعه اذا كان
+
+577
+00:45:17,840 --> 00:45:20,440
+متصل في مستشفى بسميه hospital lab اذا ما .. اذا
+
+578
+00:45:20,440 --> 00:45:27,910
+ماكان يعني private فبسميه private labأقسام المختبر
+
+579
+00:45:27,910 --> 00:45:32,410
+و أيضا أنواعه حسب الخدمة اللي بيقدموها، في حاجة
+
+580
+00:45:32,410 --> 00:45:35,130
+بسميها community business lab يعني مختبر الرعاية
+
+581
+00:45:35,130 --> 00:45:40,530
+الأولية بجري بحوصات بسيطة جدا اللي هي يعني ال ..
+
+582
+00:45:42,740 --> 00:45:46,840
+فحوصات urine و stool و نسبة الدم و يعني فحوصات
+
+583
+00:45:46,840 --> 00:45:53,980
+بسيطة جدا district hospital lab يعني هو مثلا زي
+
+584
+00:45:53,980 --> 00:45:58,800
+الفحوصات اللي بتكون موجودة في المدينة يعني مثلا
+
+585
+00:45:58,800 --> 00:46:02,160
+انا عندي قرية تابعة على مدينة هذه القرية فيها
+
+586
+00:46:02,160 --> 00:46:07,380
+مختبر صغيرالمختبر الصغير هذا إله district hospital
+
+587
+00:46:07,380 --> 00:46:12,400
+مختبر آخر بعيد عنه موجود في المدينة متصل معاه يعني
+
+588
+00:46:12,400 --> 00:46:17,560
+في تواصل ما بينهم في communication إجت عينة بتتطلب
+
+589
+00:46:17,560 --> 00:46:21,540
+فحوصات دقيقة ومتطورة بيرسلوها على ال district
+
+590
+00:46:21,540 --> 00:46:25,820
+hospital لاب علشان يشتغلوها، تمام؟ فإذا ال
+
+591
+00:46:25,820 --> 00:46:29,160
+district hospital lab زي الفحص .. زي المختبر اللي
+
+592
+00:46:29,160 --> 00:46:33,160
+في المدينة تابع لمختبر في القرية، تمام؟ال regional
+
+593
+00:46:33,160 --> 00:46:39,980
+hospital lab يعني مثلا زي مختبر اللي مثلا عندي عدة
+
+594
+00:46:39,980 --> 00:46:45,240
+مدن إلها مختبر مرجع في العاصمة، تمام؟ يعني أكبر
+
+595
+00:46:45,240 --> 00:46:49,220
+شوية من ال district hospital lab و أيضا أو موجود
+
+596
+00:46:49,220 --> 00:46:53,800
+في إقليم معين أو في منظومة معينة فبالتالي بيكون
+
+597
+00:46:53,800 --> 00:46:58,660
+مرجع له عدة مختبرات أصغر منهال central أو ال
+
+598
+00:46:58,660 --> 00:47:02,200
+public health lab زي مختبرات منظمة الصحة العالمية
+
+599
+00:47:02,200 --> 00:47:07,160
+طبعا هذا إيش بتشرف على مختبر مش على جد قرى و مدن
+
+600
+00:47:07,160 --> 00:47:12,260
+لأ على جد دول تمام و بتتابع شغل ال public health
+
+601
+00:47:12,260 --> 00:47:18,700
+إيش يعني؟ يعني بتتابع مستوى الصحة العامة ليعني
+
+602
+00:47:18,700 --> 00:47:25,320
+شريحة كتير كبيرة من الناسطيب إيش يعني lab
+
+603
+00:47:25,320 --> 00:47:28,260
+informatics؟ ال lab informatics طبعا اللي جيتها
+
+604
+00:47:28,260 --> 00:47:32,380
+الزمان كان شغل المختبرات النتائج يجيني طلب الفحص
+
+605
+00:47:32,380 --> 00:47:37,020
+على ورقة أنا أجري الفحص و أسلم النتائج أيضا على
+
+606
+00:47:37,020 --> 00:47:40,900
+ورقة أخرى و أبعتها فكان في تبادل ورق ما بين
+
+607
+00:47:40,900 --> 00:47:45,380
+الأقسام هلأ إحنا بنشوف كل شي صار محاوسب حتى
+
+608
+00:47:45,380 --> 00:47:51,500
+تعليمنا الأيام هذه صار محاوسب فبالتالي ال .. ال ..
+
+609
+00:47:51,970 --> 00:47:56,750
+ال lab informatics هو عبارة عن منظومة محوثبة من
+
+610
+00:47:56,750 --> 00:48:02,390
+خلالها بيتم تبادل exchange لل data هي عبارة عن
+
+611
+00:48:02,390 --> 00:48:06,350
+software program and computer هي برامج وأجهزة
+
+612
+00:48:06,350 --> 00:48:10,910
+computer بي .. وظيفتها إيش تعملي exchange لل data
+
+613
+00:48:11,650 --> 00:48:16,530
+طبعا data لاقاش يعني معلومات لاقاش اللي بنتبدلها
+
+614
+00:48:16,530 --> 00:48:21,690
+حسب عن المرضى و الفخصات اللي انطلبت لهم و النتائج
+
+615
+00:48:21,690 --> 00:48:26,270
+اللي اتسجلت لهم و هذه كلها بسميها lab informatics
+
+616
+00:48:26,270 --> 00:48:31,770
+systemطيب مثال عليها، هلأ بيجي .. هلأ صار احنا
+
+617
+00:48:31,770 --> 00:48:34,410
+بيجينا في المختبرات .. هذا الاشي اللي ان شاء الله
+
+618
+00:48:34,410 --> 00:48:38,150
+لما تروحوا على مستشفيات و تتدربوا، راح تشوفوه
+
+619
+00:48:38,150 --> 00:48:42,510
+بعينكم طلب الفحص بيجي من خلال إشعار على كمبيوتر
+
+620
+00:48:42,510 --> 00:48:50,870
+المختبر، تمام؟ال .. المختبر .. بيجي الكاتب بيفوت
+
+621
+00:48:50,870 --> 00:48:56,630
+على الطلبات الفحوصات و بيعرف من خلالها أخصاء
+
+622
+00:48:56,630 --> 00:49:00,770
+المختبر إيش الفحوصات المطلوبة على العينات و
+
+623
+00:49:00,770 --> 00:49:05,870
+بيجريهاطبعا طلعت النتائج على نفس الطلب اللي إجا
+
+624
+00:49:05,870 --> 00:49:10,410
+على الكمبيوتر بيجي أخسها المختبر و بيسجل النتائج
+
+625
+00:49:10,410 --> 00:49:13,490
+اللي طلعت لكل عينة كل عينة إلها بيكون طلب على
+
+626
+00:49:13,490 --> 00:49:18,730
+الكمبيوتر فبعبيها و باعتمدها الدكتور بيروح بيفتح
+
+627
+00:49:18,730 --> 00:49:25,900
+على نفس الملفتمام؟ و بيقرأ نتائج المختبر فبالتالي
+
+628
+00:49:25,900 --> 00:49:31,500
+بيشخص و بيكتب علاج على ال computer أيضا و
+
+629
+00:49:31,500 --> 00:49:36,540
+بيعتمدوا، بيروح الصيدلاني بيفتح على ال .. نفس
+
+630
+00:49:36,540 --> 00:49:41,410
+الملف للمريضوبيشوف أشي العلاج المطلوب، بيوصف
+
+631
+00:49:41,410 --> 00:49:45,830
+وبيعطي إله، تمام؟ الكاتب بده يعطي موعد لصورة أشعة
+
+632
+00:49:45,830 --> 00:49:53,210
+أو موعد لفحص يعني تصوير، أي فحصرانين، أي إشي من
+
+633
+00:49:53,210 --> 00:49:57,550
+خلال أنه يعني بيفوت على صفحة ال .. على ملف المريض
+
+634
+00:49:57,550 --> 00:50:02,700
+وبيكتبله الموعد داخل هذا الملفأصبح المريض صار
+
+635
+00:50:02,700 --> 00:50:06,460
+عندنا عبارة عن إيش؟ عبارة عن رقم ملف لازم يكون هو
+
+636
+00:50:06,460 --> 00:50:11,300
+المريض محتفظ فيه و من خلاله إحنا بنفوت على هذا
+
+637
+00:50:11,300 --> 00:50:17,460
+الملف بنلاقي كل المعلومات كله بيسجل معلوماته على
+
+638
+00:50:17,460 --> 00:50:21,060
+هذا الملف فصار إيش في عندي? exchange that data أنا
+
+639
+00:50:21,060 --> 00:50:26,380
+لما بحصل على رقم المريض الطبي رقم ملفه بفوت على
+
+640
+00:50:26,380 --> 00:50:29,600
+هذا البرنامج على ال software program و ال computer
+
+641
+00:50:30,360 --> 00:50:34,460
+و من خلالها بعرف إيش انطلب له فحوصات، إيش أنا سجلت
+
+642
+00:50:34,460 --> 00:50:37,960
+نتائج، إيش الدكتور كتاب له علاج، إيش القصيدلاني
+
+643
+00:50:37,960 --> 00:50:42,360
+صرف له علاج، طبع السكرتارية الطبية إمتى أعطاه موعد
+
+644
+00:50:42,360 --> 00:50:48,380
+مراجعة، كإمتى انكتبله موعد صور أشعة، إمتى انكتبله
+
+645
+00:50:48,380 --> 00:50:53,280
+موعد مثلا جلسات علاج طبيعي، و هكذا، كلها هذه عبارة
+
+646
+00:50:53,280 --> 00:51:00,020
+عن إيش بنسميها informatics، تمام؟الجزء اللى بيتعلق
+
+647
+00:51:00,020 --> 00:51:04,900
+بشغلى انا مثلا امتى اعطانى عينة و امتى انا صح
+
+648
+00:51:04,900 --> 00:51:08,860
+اشتغلتها و امتى سجلت نتائجى و اش النتائج اللى
+
+649
+00:51:08,860 --> 00:51:13,220
+سجلتها و اش الفحوصات اللى كانت مطلوبة بسميها lab
+
+650
+00:51:13,220 --> 00:51:17,920
+informatics لإنها معلومات بتخص شغلى انا بتخص شغلى
+
+651
+00:51:17,920 --> 00:51:22,940
+ك lab فبسميها lab informatics طبعا هى معناه اش
+
+652
+00:51:22,940 --> 00:51:25,400
+يعنى ال informatics و اش يعنى ال lab informatics
+
+653
+00:51:25,400 --> 00:51:31,620
+systemتمام؟ طيب فهذا ال system إيش .. إيش مميزاته
+
+654
+00:51:31,620 --> 00:51:37,480
+أصبح يسهل عمل المختبر،
+
+655
+00:51:37,480 --> 00:51:42,170
+صار في عندي سهولة لطلب الفحوصات لكل مريضصار في
+
+656
+00:51:42,170 --> 00:51:45,290
+عندى tracking، إيش يعني tracking؟ يعني متابعة،
+
+657
+00:51:45,290 --> 00:51:50,090
+أشوف المريض وصلت العينة للمختبر ولا لأ، إشتغلوها
+
+658
+00:51:50,090 --> 00:51:53,350
+أو لأ، طلعت النتائج، سجلوا النتائج أو لأ، هذه كلها
+
+659
+00:51:53,350 --> 00:51:55,930
+عبارة عن متابعة من خلال إن أنا ضغطت زر، بس أشوف
+
+660
+00:51:55,930 --> 00:52:00,390
+ملف المريض إيش انكتب عليه معلومات أو بياناتو أيضا
+
+661
+00:52:00,390 --> 00:52:04,490
+تضمن help guarantee a better quality for result
+
+662
+00:52:04,490 --> 00:52:09,330
+يعني بتضمنلي انه انا يكون في عندى جودة عالية
+
+663
+00:52:09,330 --> 00:52:13,870
+للنتائج بدل ما انا اضل اكتب على ورق و الورق هذا
+
+664
+00:52:13,870 --> 00:52:21,730
+يضيع يتلف نفقدها فبالتالي نضيع النتائج تبعتنا
+
+665
+00:52:21,730 --> 00:52:27,710
+بتساعد الدكاترة من ان هم ايشيعملوا تشريك على ما تم
+
+666
+00:52:27,710 --> 00:52:30,930
+كتابة على ملف المريض وبالتالي بتساعدهم في التشخيص
+
+667
+00:52:30,930 --> 00:52:38,030
+ووصف العلاج. هك بنكون خلصنا جزئية ال medical
+
+668
+00:52:38,030 --> 00:52:41,410
+laboratories. طبعا تعرفنا إيش يعني مختبر، إيش يعني
+
+669
+00:52:41,410 --> 00:52:45,430
+clinical أو medical laboratory وإيش إحنا وظيفتنا
+
+670
+00:52:45,430 --> 00:52:49,910
+وأنواع المختبرات مع أقسامها ونوع الخدمات اللي
+
+671
+00:52:49,910 --> 00:52:57,430
+بتقدمها داخل المختبرات.هنحكي جزئية بسيطة عن ال ..
+
+672
+00:52:57,430 --> 00:53:04,710
+ال lab safety طبعا جزئية بسيطة جدا ه .. نختصرها ..
+
+673
+00:53:04,710 --> 00:53:08,770
+يعني هذه مختصرة موضوع المحاضرة العملية هيكون
+
+674
+00:53:08,770 --> 00:53:14,810
+باسهاب أكتر عنها طبعا ال lab safety ruleقواعد
+
+675
+00:53:14,810 --> 00:53:19,670
+السلام والأمان داخل المختبر طبعا هي اشي مهم جدا هي
+
+676
+00:53:19,670 --> 00:53:26,190
+major aspect اشي مهم جدا في كل مختبر كل طالب طبعا
+
+677
+00:53:26,190 --> 00:53:32,530
+في المختبر لازم يتبع القواعد السلام والأمان لكل
+
+678
+00:53:32,530 --> 00:53:37,890
+تجربة بيجريهاطبعا ال lab safety rule من القواعد
+
+679
+00:53:37,890 --> 00:53:42,610
+السلامة و الأمان حتى العلامات و الإشارات احنا
+
+680
+00:53:42,610 --> 00:53:50,110
+أحيانا بنكون كاتبين ورق معالجينه او رموز على ال ..
+
+681
+00:53:50,110 --> 00:53:54,650
+معالجينه على ال أنابيب على ال bottles الذجاجات
+
+682
+00:53:54,650 --> 00:53:59,130
+طبعتناعلى الحيط على الخزانات عشان انحذر ان هنا في
+
+683
+00:53:59,130 --> 00:54:03,170
+اشي حارق هنا في اشي ممكن يأذي الشخص هنا اشي ..
+
+684
+00:54:03,170 --> 00:54:09,350
+فبالتالي لازم احنا ايضا نكون بنعرف هاي الشغلات و
+
+685
+00:54:09,350 --> 00:54:13,410
+بنتابعها سواء كانت قواعد او ان انا التزم
+
+686
+00:54:13,410 --> 00:54:16,790
+بالتعليمات اللي مكتوبة على الخزانة مثلا مش لازم
+
+687
+00:54:16,790 --> 00:54:20,350
+انا .. اي اشي داخل الخزانة هادى من هو انا مش لابسة
+
+688
+00:54:20,350 --> 00:54:23,270
+gloves فبالتالي انا بدى اتبع هاي الشغلات ليش؟ عشان
+
+689
+00:54:23,270 --> 00:54:27,100
+أحمي نفسيو أحمي ال classmates تبعوني زملائي اللي
+
+690
+00:54:27,100 --> 00:54:31,220
+داخل الفصل أو داخل المحاضرة طبعا إيش هي ال lab
+
+691
+00:54:31,220 --> 00:54:37,020
+safety rules بشكل عام؟أنا داخل المختبر لازم ألتزم
+
+692
+00:54:37,020 --> 00:54:41,360
+بإن أنا ألبس ال lab coat لأ ألبس ال gloves و
+
+693
+00:54:41,360 --> 00:54:45,380
+أحيانا بعد ال .. ال .. العمل المخبر يحتاج إن أنا
+
+694
+00:54:45,380 --> 00:54:50,920
+ألبس goggles نضارات و ألبس shoes تمام صندل لأ كعب
+
+695
+00:54:50,920 --> 00:54:57,660
+لأ الأشياء اللي ت .. اللي هتتحقق معها الأناقة مش
+
+696
+00:54:57,660 --> 00:55:01,220
+ضرورية داخل المختبر أناقتك بي close shoes لازم
+
+697
+00:55:01,220 --> 00:55:05,570
+تكون موجودةيعني أنا بدي .. بدي أناقتي ولا سلامتي؟
+
+698
+00:55:05,570 --> 00:55:09,590
+لأ سلامتي أبدا من الأناقة، تمام؟ فبالتالي لازم انه
+
+699
+00:55:09,590 --> 00:55:14,310
+ال shoes يكون مغلق، فال gloves اشي .. اشي مهم
+
+700
+00:55:14,310 --> 00:55:18,210
+تمام؟ ال lab coat مطلوب، ال goggles حكينا بعض
+
+701
+00:55:18,210 --> 00:55:23,100
+الشغل محتاجة يعني إذا كانالتفاعل متطاير أو ممكن
+
+702
+00:55:23,100 --> 00:55:27,280
+يعطي شرر أو ممكن يعطي رزاد فبالتالي أنا لازم ألبس
+
+703
+00:55:27,280 --> 00:55:32,700
+goggles نضارات علشان أحمي نفسي طبعا ال issues لازم
+
+704
+00:55:32,700 --> 00:55:38,740
+يكون مغلق تمام مش لازم يكون مفتوح و الأشياء
+
+705
+00:55:38,740 --> 00:55:43,140
+المريحة و العملية و الأنيقة و ال .. ال .. ال new
+
+706
+00:55:43,140 --> 00:55:47,560
+fashion هاي نتركنا منها أنا قدنا نحققها ان شاء
+
+707
+00:55:47,560 --> 00:55:50,080
+الله برا المختبر و داخل المختبر سلامتنا أو لا
+
+708
+00:55:52,070 --> 00:55:58,370
+الغوايش طبعا don't wear غوايش ماشي ال .. ال ..
+
+709
+00:55:58,370 --> 00:56:01,770
+الناس اللي معودة نفسها على ال accessories
+
+710
+00:56:01,770 --> 00:56:06,570
+الاكسسوارات و مابتطلعش من غيرها هتلاقي حالها ان هي
+
+711
+00:56:06,570 --> 00:56:12,850
+بتتغلب داخل المختبر فبالتالي مش لازم ان هي ت ..
+
+712
+00:56:12,850 --> 00:56:16,970
+يعني تلبسها و أيضا الملابس الواسعة و الفطفادة الكم
+
+713
+00:56:16,970 --> 00:56:21,780
+اللي نازل زي الفراشة و ال ..و الملابس الواسعة و
+
+714
+00:56:21,780 --> 00:56:26,680
+الشيفونات و الحاجات هذه برضه كمان مش مطلوبة طبعا
+
+715
+00:56:26,680 --> 00:56:31,920
+مش لازم انا يعني شايفين هذا الشخص قاعد بيشتغل في
+
+716
+00:56:31,920 --> 00:56:34,960
+منطقة هذه .. هذه اسمها safety cabinets يعني اللي
+
+717
+00:56:34,960 --> 00:56:38,180
+بيفوت جوا لازم يكون معقم و لا و أحيانا بتكون ..
+
+718
+00:56:38,180 --> 00:56:40,820
+بنكون بنشتغل في أشياء معضية داخل .. في الداخل هذه
+
+719
+00:56:40,820 --> 00:56:43,910
+ال cabinetتمام؟ فهو .. هو لابس ال gloves يعني كان
+
+720
+00:56:43,910 --> 00:56:47,110
+متعامل مع الأشياء اللي في الداخل مسك التليفون
+
+721
+00:56:47,110 --> 00:56:50,650
+الخاص فيه و هذا غلط فأنا .. و أنا لابسة ال gloves
+
+722
+00:56:50,650 --> 00:56:54,950
+و أنا بشتغل في .. يعني لمست العينات و لمست الأدوات
+
+723
+00:56:54,950 --> 00:56:59,010
+حتى المواد الكيميائية مش لازم أرجع في نفس ال .. ال
+
+724
+00:56:59,010 --> 00:57:03,850
+gloves أو في نفس الأيدالمس الأقلام و التليفونات و
+
+725
+00:57:03,850 --> 00:57:12,170
+حتى مقابد الغرف مش لازم طبعا يفضل إن أنا أخلع ال
+
+726
+00:57:12,170 --> 00:57:16,170
+gloves و أغسل إيديا و بعدين أروح أخد أغراضي و إلا
+
+727
+00:57:16,170 --> 00:57:20,950
+بكون أنا ساعتها أشبع باخد مواد معضية أو مواد سامة
+
+728
+00:57:20,950 --> 00:57:24,110
+و بروح بحطها على أغراضي علشان أنشرها بعد هيك بعد
+
+729
+00:57:24,110 --> 00:57:28,350
+ما أطلع المختبر أنشرها everywhereطبعا أعطى زميلاتى
+
+730
+00:57:28,350 --> 00:57:31,390
+شوية من تخصصى و زميلاتى اللى من التخصص التانى
+
+731
+00:57:31,390 --> 00:57:37,190
+أعطهم شوية مواد .. مواد سامة و .. معدية و هكذا
+
+732
+00:57:37,190 --> 00:57:43,230
+يعني بكون انا ايهاش برج ارسال بنشر هذا غلط طبعا
+
+733
+00:57:43,230 --> 00:57:47,580
+لازم ناخد حضرناالأقلم طبعا إذا أنا بكتب فيها و هي
+
+734
+00:57:47,580 --> 00:57:51,680
+موجودة عند الأوضاع المختبر مش لازم أحطها بالثم أو
+
+735
+00:57:51,680 --> 00:57:56,020
+إن أنا خلاص يعني حاسيت إن الجلم اتلوث ما .. يعني
+
+736
+00:57:56,020 --> 00:58:02,450
+ما أرجع أحطه مع باقي أغراضي أو داخل الشنطةمااتريكش
+
+737
+00:58:02,450 --> 00:58:07,510
+ال benz burner شغال و أروح أتحدث أو أ .. أتفسح أو
+
+738
+00:58:07,510 --> 00:58:12,290
+أ .. أروح في .. أشغل شغل تاني لإنه ممكن يعني
+
+739
+00:58:12,290 --> 00:58:16,890
+يتأيدي بحرائق تمام؟ أول ما أخلص شغلي بسكر ال benz
+
+740
+00:58:16,890 --> 00:58:20,590
+burner اللي هو لها ال benz علشان أضمن إنه مايكونش
+
+741
+00:58:20,590 --> 00:58:24,830
+في عندي حرائق و طبعا أتأكد إنه الغاز المقبض
+
+742
+00:58:24,830 --> 00:58:28,010
+الرئيسي للغاز أنا كمان أغلقته عشان مايصيرش في عندي
+
+743
+00:58:28,010 --> 00:58:32,270
+تسريب غاز لإنه في حوادث كتيرة صارتانه مثلا يكون
+
+744
+00:58:32,270 --> 00:58:35,250
+فيه تسريب للغاز مع إضاءة الكهربا يصير في حرائق.
+
+745
+00:58:36,170 --> 00:58:39,070
+طبعا الناس اللي .. الحمد لله نعمة الإسلام احنا
+
+746
+00:58:39,070 --> 00:58:46,090
+بنغطي شعارنا ولكن الناس اللي مش محجبة لازم ما تترك
+
+747
+00:58:46,090 --> 00:58:52,690
+الشعر سايح وسايب لأ يفضل ربطه او رفعه علشانمايكونش
+
+748
+00:58:52,690 --> 00:58:56,530
+مش .. يعني مثلا مايحترقش او يصير فيه contamination
+
+749
+00:58:56,530 --> 00:59:02,910
+فيصير في خطر hazard او contamination تمام؟ don't
+
+750
+00:59:02,910 --> 00:59:07,150
+food or drink water in the lab مش لازم ناكل او
+
+751
+00:59:07,150 --> 00:59:12,330
+نشرب داخل المختبر او بجانب اماكن العمل لإنه ممكن
+
+752
+00:59:12,330 --> 00:59:16,230
+تتلوث هاي المأكولات و المشروبات و بالتالي انه نؤذي
+
+753
+00:59:16,230 --> 00:59:20,960
+أنفسناprotect your safety و أيضا كمان مش لازم ان
+
+754
+00:59:20,960 --> 00:59:24,640
+انا يجي على بالي هيك من الفضول اتذوق اشي داخل
+
+755
+00:59:24,640 --> 00:59:29,860
+المختبر protect your hand safety لازم مثلا لما انا
+
+756
+00:59:29,860 --> 00:59:34,880
+ألمس اشي ساخنمثلا ال flask ساخن ألبس gloves عازل
+
+757
+00:59:34,880 --> 00:59:41,820
+للحرارة أو أمسكه من خلال ملقط تمام؟ كيف أيضا ب ..
+
+758
+00:59:41,820 --> 00:59:46,820
+بحافظ على ال .. ال .. إيديا سلامة إيديا أن أنا ما
+
+759
+00:59:46,820 --> 00:59:51,140
+أمسك الأشياء ال .. الزجاج المكسور أو الأدوات
+
+760
+00:59:51,140 --> 00:59:54,940
+الحادة بإيدي على طول لأ لازم أن أنا أتعامل معها
+
+761
+00:59:54,940 --> 01:00:00,510
+بحذر أيضا أتابع أعمل wash hand باستمرارهذا أيضا
+
+762
+01:00:00,510 --> 01:00:05,370
+بيحافظلي على سلامة اليدان ال electrical safety هو
+
+763
+01:00:05,370 --> 01:00:10,710
+أول ما بخلص شغل بجهاز computer بفصل الكبش تبعه من
+
+764
+01:00:10,710 --> 01:00:19,430
+الكهربا و طبعا ممنوع ألامس أو أتركالأسلاك والأحبال
+
+765
+01:00:19,430 --> 01:00:24,690
+الكهربائية مكشوفة طبعا ال chemical safety مش لازم
+
+766
+01:00:24,690 --> 01:00:33,030
+أنا ألمس أحس أو أشم أو أتذوق أي مادة كيميائية داخل
+
+767
+01:00:33,030 --> 01:00:39,500
+المختبر تمام؟ و أيضا مش لازمأخلط مواد كيميائية هيك
+
+768
+01:00:39,500 --> 01:00:43,200
+يطلع فيا حس ال .. الحس الكيميائي عندي و أبلش أخلط
+
+769
+01:00:43,200 --> 01:00:47,020
+مواد كيميائية مدام أنا ما .. ما أعطيتكم تعليمات
+
+770
+01:00:47,020 --> 01:00:52,280
+أنه اضيفي مادة ألف على بها انت ما تعملي إضافة،
+
+771
+01:00:52,280 --> 01:00:55,550
+تمام؟لأن خلط المواد الكيميائية مع بعضها البعض ممكن
+
+772
+01:00:55,550 --> 01:00:59,430
+تكون نتائجه يعني أو عواقب وخيمة keep lids on
+
+773
+01:00:59,430 --> 01:01:04,830
+chemical container طبعا المواد sorry المغطية لازم
+
+774
+01:01:04,830 --> 01:01:08,130
+انا اترك الغطاية عليها keep lids ال lids يعني
+
+775
+01:01:08,130 --> 01:01:11,090
+الغطاة لازم اضل احط .. احطها على ال chemical
+
+776
+01:01:11,090 --> 01:01:14,290
+container علشان في النهاية في مواد ممكن تمتسر
+
+777
+01:01:14,290 --> 01:01:19,590
+وطوبها فيتغير تركيزهافي مواد ممكن أنها هي تمتص
+
+778
+01:01:19,590 --> 01:01:25,410
+الوطوبة فتصير مشتعلة، في مواد ممكن تتبخر فبالتالي
+
+779
+01:01:25,410 --> 01:01:28,530
+أنا لازم أضل حاطة ال lids اللي هي الغطايا على ال
+
+780
+01:01:28,530 --> 01:01:33,390
+container يعني أسكر منيهاdon't take any culture
+
+781
+01:01:33,390 --> 01:01:38,350
+out of the lab إذا أنا بشتغل بمزارع تمام مش لازم
+
+782
+01:01:38,350 --> 01:01:43,570
+هذه أطباق عبارة عن مزرعة فيها مواد مغذية و بتنمو
+
+783
+01:01:43,570 --> 01:01:46,930
+عليها البكتيريا أو الفطريات و من خلالها أنا بتعرف
+
+784
+01:01:46,930 --> 01:01:52,410
+على الفطريات أو البكتيريا اللي ممكن تسبب عدوى تمام
+
+785
+01:01:52,410 --> 01:01:57,810
+ف .. و هذه أيضا عبارة عن culture ولكن هنا مثلا
+
+786
+01:01:57,810 --> 01:02:01,590
+المادة الغذائية صلبة هنا المادة الغذائية سائلةطبعا
+
+787
+01:02:01,590 --> 01:02:07,330
+هذه
+
+788
+01:02:07,330 --> 01:02:10,810
+ال cultures هذه المواد المغذية اللي نامي عليها
+
+789
+01:02:10,810 --> 01:02:16,270
+بكتيريا أنا ممنوع أخدها برا المختبر طبعا؟ ليش؟
+
+790
+01:02:16,270 --> 01:02:21,050
+علشان ممكن تنتوج و تنكسر يعني في النهاية تنتشر
+
+791
+01:02:21,050 --> 01:02:27,060
+العدوى خارج المختبر لأ فبالتالي لازمسواء كانت ال
+
+792
+01:02:27,060 --> 01:02:31,840
+.. ال .. نتيجة ال .. يعني نتيجة المزرعة هاي
+
+793
+01:02:31,840 --> 01:02:35,780
+البكتيريا اللي نمت عندى معضية أو غير معضية بتسبب
+
+794
+01:02:35,780 --> 01:02:39,360
+أمراض أو ما بتسبب أمراض كلها بتتعامل على أنها هي
+
+795
+01:02:39,360 --> 01:02:41,960
+pathogenic إيش يعني أتعامل .. أعملها على أنها
+
+796
+01:02:41,960 --> 01:02:46,120
+pathogenic؟ على أنها ممرضة تمام؟ يعني ممنوع أنا
+
+797
+01:02:46,120 --> 01:02:49,620
+خلاص أضمني هاي البكتيريا بتسببش أمراض مصرا .. خلاص
+
+798
+01:02:49,620 --> 01:02:53,820
+أ ..اشتغل فيها لأ don't trust بكتيريا ماتفقيش في
+
+799
+01:02:53,820 --> 01:02:57,500
+البكتيريا لإن هي فجأة انت ممكن تتحول من non
+
+800
+01:02:57,500 --> 01:03:02,900
+-pathogenic لpathogenic فيعني و في كتير بكتيريا
+
+801
+01:03:02,900 --> 01:03:06,460
+بنسميها البكتيريا الانتهازية اللي هي تنتهز فرصة هي
+
+802
+01:03:06,460 --> 01:03:12,690
+بتكونش ممرضة ولكن تنتهز فرصة بتصير ممرضةايضا ال
+
+803
+01:03:12,690 --> 01:03:17,650
+liquid culture اللي هي ال culture السائلة هذه لازم
+
+804
+01:03:17,650 --> 01:03:21,090
+انها تكون داخل ال rack موجودة هذا ال rack اللي هو
+
+805
+01:03:21,090 --> 01:03:24,770
+الحامل تبعها و تكون موجودة على ال bench إلى الداخل
+
+806
+01:03:24,770 --> 01:03:28,270
+ان انا لما امر مثلا ماتجعش تنكب تنكسر مني تنتشر
+
+807
+01:03:28,270 --> 01:03:32,930
+العدوى او تنتشر البكتيريا اللي في .. على الأرض و
+
+808
+01:03:32,930 --> 01:03:37,190
+ايضا مش لازم انا يعني انه المهم ما تنكسرش و تسرب
+
+809
+01:03:38,290 --> 01:03:43,690
+عندي don't engage in practical jokes or horseplay
+
+810
+01:03:43,690 --> 01:03:47,830
+in the lab يعني مثلا ما تحاولوش أنكوا تنصبوا لبعض
+
+811
+01:03:47,830 --> 01:03:54,310
+فخ داخل المختبر أو أنكوا انتوا تعملوا يعني تطلعوا
+
+812
+01:03:54,310 --> 01:03:59,930
+حس الدعابة عندكم و تبدوا تعملوا مع بعض مجالب فتحطي
+
+813
+01:03:59,930 --> 01:04:06,510
+لصحبيتك مادة غريبة ولاتخليها تشم أو انك انت مثلا
+
+814
+01:04:06,510 --> 01:04:12,350
+ترشيها عليها او تخوفيها تجيبيلها اشي غريب داخل
+
+815
+01:04:12,350 --> 01:04:18,050
+المختبر لإن ممكن اي ركضة تركض مثلا و هي باللاوعي
+
+816
+01:04:18,050 --> 01:04:23,370
+تبعها خايفة او تنكسر او ينكب عليها اشي ممكن اذا
+
+817
+01:04:23,370 --> 01:04:28,630
+انت بدلتيلها مادة بمادة اخرى يعني يلحقها اذا او
+
+818
+01:04:28,630 --> 01:04:33,070
+اذا مثلا طلع .. يعني حكيتيلهاهي ماكنتش سامة ..
+
+819
+01:04:33,070 --> 01:04:37,570
+المس حكت شمه، دوجه و هي المس ماحكتش .. يعني انت
+
+820
+01:04:37,570 --> 01:04:42,150
+بتتركني روحتيها يعني كانت عندنا في سنة من السنوات
+
+821
+01:04:42,150 --> 01:04:47,170
+صارت .. كنا بنشتغل في فحص .. في مختبر الكيميا
+
+822
+01:04:47,170 --> 01:04:53,310
+الحيوية و كانت .. كنا بنستخدم الacetic acid المركز
+
+823
+01:04:53,310 --> 01:04:59,450
+طبعا خالي يعني مركز جدا فال .. البنت حكت لزملتها
+
+824
+01:04:59,450 --> 01:05:07,450
+شمهفيعني زميلتها اشتمته فيعني قعدت فترة طويلة يعني
+
+825
+01:05:07,450 --> 01:05:12,170
+تقريبا نص ساعة اختناق عندها يعني بتسعى للبنت بشكل
+
+826
+01:05:12,170 --> 01:05:15,790
+قوي جدا و مش قادرة تشم و هو اصلا يعني لما هي شمته
+
+827
+01:05:15,790 --> 01:05:23,210
+عمل تهيج للانسجة في الداخل فيعني احنا البنت ..
+
+828
+01:05:23,270 --> 01:05:28,790
+احتجنا ان احنا مثلا نخليها تشرب مي و انها .. حطها
+
+829
+01:05:28,790 --> 01:05:33,330
+في مكان تهوية فيه كتيرة و نفكلها الشالة و يعني
+
+830
+01:05:33,330 --> 01:05:38,390
+عملنا لها إسعافات أولية لحتى نخلصها من أثر أنها هي
+
+831
+01:05:38,390 --> 01:05:43,370
+شمّة حمض قوي فبالتالي رجاء ما تمزحوا مع بعض هيك
+
+832
+01:05:43,370 --> 01:05:46,550
+مزحات جديدة، حس الدعابة برا المختبر، مش داخل
+
+833
+01:05:46,550 --> 01:05:52,140
+المختبرطبعا keep non-essential book and clothing
+
+834
+01:05:52,140 --> 01:05:54,920
+far away from your working area يعني أنت ماعندك
+
+835
+01:05:54,920 --> 01:05:58,160
+الكتب و الملازم و الأشياء مش لازم تكون موجودة على
+
+836
+01:05:58,160 --> 01:06:01,340
+نفس ال bench اللي بتشتغلي فيه لأ ممكن أنت مثلا
+
+837
+01:06:01,340 --> 01:06:06,140
+تشتغلي على ال bench أو على الأماكن المخصصة للعمل و
+
+838
+01:06:06,140 --> 01:06:09,200
+لما تطلع معاكي نتيجة على طول تشلح ال gloves تغسل
+
+839
+01:06:09,200 --> 01:06:12,540
+يديك و تروح تسجل النتيجة تبعك على دفترك هيك أكثر
+
+840
+01:06:12,540 --> 01:06:18,300
+أمانا و سلاما بحيث أن دفاترك تبقى نظيفة تبقى مش ..
+
+841
+01:06:18,480 --> 01:06:22,900
+مولوثة فبالتالي تظلي محافظة على سلامتك و سلامة أي
+
+842
+01:06:22,900 --> 01:06:27,300
+حدا ممكن يستخدم شنتك او يستخدم ي .. يلامس أغراضك
+
+843
+01:06:27,300 --> 01:06:31,220
+او يلامسك انت بشكل مباشر او يحتك فيك بشكل مباشر
+
+844
+01:06:31,220 --> 01:06:36,320
+طيب بعد ما احنا نخلص شغلنا لازم انه انا انظف ال
+
+845
+01:06:36,320 --> 01:06:42,360
+bench ب disinfectant طبعا و يفضل انه قبل و بعد
+
+846
+01:06:42,360 --> 01:06:45,880
+الشغل انظف او اعقم ال bench ب disinfectant
+
+847
+01:06:47,740 --> 01:06:50,960
+disinfectant يعني مادة معاقمة طبعا بنستخدم احنا
+
+848
+01:06:50,960 --> 01:06:56,020
+detol أحيانا نستخدم ethanol كحول ممكن bleach عشرة
+
+849
+01:06:56,020 --> 01:07:00,980
+في المية chlorine محلول فبالتالي حسب الشيء المتوفر
+
+850
+01:07:00,980 --> 01:07:07,400
+و بنستخدمه قبل و بعد لتنظيف و تعقيم البنشاتdispose
+
+851
+01:07:07,400 --> 01:07:11,560
+of waste products according to instructions بعض ال
+
+852
+01:07:11,560 --> 01:07:18,460
+.. طبعا إن أنا أتخلص بال waste يعني بالفضلات في
+
+853
+01:07:18,460 --> 01:07:23,380
+مكانها المناسب في فضلات أتخلص فيها بالسلة العادية
+
+854
+01:07:23,380 --> 01:07:28,340
+في فضلات ممكن تكون ذات إشعاع يعني مواد مشعة فأنا
+
+855
+01:07:28,340 --> 01:07:31,240
+بدأ أتخلص فيها داخل صناديق و الصناديق هذي أحكم
+
+856
+01:07:31,240 --> 01:07:35,260
+أغلاقها أو أحطها في صندوق رصاصو بعدين يعني يتم ..
+
+857
+01:07:35,260 --> 01:07:40,860
+يعني اتلفها بشكل معين، في مواد معضية أو ملوثة
+
+858
+01:07:40,860 --> 01:07:46,120
+ببكتيريا أو فيروسات أو فطريات فبالتالي لما أجي بدي
+
+859
+01:07:46,120 --> 01:07:51,440
+أتلفها بدي أحطها في كيسو الكيس هذا بده يفوت على
+
+860
+01:07:51,440 --> 01:07:55,460
+جهاز التعقيم ال autoclave و بعدين يطلع .. بعدين
+
+861
+01:07:55,460 --> 01:08:00,240
+أتخلص منه، إذا أنا ما أقدر كل الفضلات عندي أرميها
+
+862
+01:08:00,240 --> 01:08:02,580
+بنفس ال container، في عندي container بده يفوت على
+
+863
+01:08:02,580 --> 01:08:05,980
+التعقيم، في container بده يفوت على التعامل مع
+
+864
+01:08:05,980 --> 01:08:08,880
+المواد المشعة، في container بده أنا خاص بأتلفه
+
+865
+01:08:08,880 --> 01:08:13,380
+عادي يعني مع نفايات عاديةفبالتالي كل نوع فضلات
+
+866
+01:08:13,380 --> 01:08:15,600
+لازم يكون في إيه له container خاص و هذا داخل
+
+867
+01:08:15,600 --> 01:08:19,040
+المختبر، فيه مثلا أدوات حادة كنا هنشتغل فيها بكرا
+
+868
+01:08:19,040 --> 01:08:24,480
+الإبر و ال ..ال .. سرنجات مثلا بصير أحكيلك أنه
+
+869
+01:08:24,480 --> 01:08:28,260
+طبعا منكون في عندي سناديج لإطلاق الأدوات الحادة
+
+870
+01:08:28,260 --> 01:08:32,020
+فهتلاقيني أنا بعطيكم تعليمات أنه بس تخلصي شغلك
+
+871
+01:08:32,020 --> 01:08:38,300
+الإبرة بترميها بهذا ال box و الباقي الأدوات اللي
+
+872
+01:08:38,300 --> 01:08:43,320
+استخدمتيهم ترميهم بالسلة فهتلاقوني أنا خلال الشغل
+
+873
+01:08:43,320 --> 01:08:46,960
+العملي هعطيكم تعليمات كيف تطلفوا الفضلات اللي احنا
+
+874
+01:08:46,960 --> 01:08:51,360
+أو ال waste اللي بيطلع من شغلناعلشان نكون محافظين
+
+875
+01:08:51,360 --> 01:08:58,290
+على السلامة و الأمان تبعتناأي مادة أو حادث بيحدث
+
+876
+01:08:58,290 --> 01:09:01,170
+داخل .. يعني أي حادث بيحدث داخل المختبر، حادث كسر،
+
+877
+01:09:01,170 --> 01:09:06,370
+أي مادة بت .. بت .. ت .. تنكب، وعاء بينكسر، أي إشي
+
+878
+01:09:06,370 --> 01:09:10,690
+بي .. بينصلا بيلامسنا، أول إشي بدنا نسجله، في
+
+879
+01:09:10,690 --> 01:09:16,490
+عندنا دفتر للحوادث، لازم يسجل بالتاريخ وال ..
+
+880
+01:09:16,490 --> 01:09:21,910
+اليوم وإيش صار بالظبط وكيف تم معالجة الخطأ ومع
+
+881
+01:09:21,910 --> 01:09:26,520
+توقيع الناس اللي موجودة داخل المختبرتمام؟ و أيضا
+
+882
+01:09:26,520 --> 01:09:30,920
+لازم أنا علشان أتخلص من آثار هذا الحادث أسأل ال
+
+883
+01:09:30,920 --> 01:09:33,340
+supervisor تبعي أسأل ال miss اللي موجودة داخل
+
+884
+01:09:33,340 --> 01:09:36,720
+المختبر miss أنا كيف أنظف هذا ..اللي انكب او اللي
+
+885
+01:09:36,720 --> 01:09:41,480
+انكسل مثلا عندي دم انكب على ال bench هذا ال blood
+
+886
+01:09:41,480 --> 01:09:46,100
+بدي انا امسحه اول حاجة ب .. ب cotton بعدين ا ..
+
+887
+01:09:46,100 --> 01:09:51,280
+انظف اعقم مكانه بكحول فبالتالي لازم يكون في عندي
+
+888
+01:09:51,280 --> 01:09:57,490
+طرق كيف اتعامل معاها مثلا انكب بحمضعلى ال bench
+
+889
+01:09:57,490 --> 01:10:00,890
+الحمض هذا مثلا لو انا بدي أدير عليه ميه ممكن يشتعل
+
+890
+01:10:00,890 --> 01:10:04,290
+لأ بروح بحطله base عشان يعادله يعمله
+
+891
+01:10:04,290 --> 01:10:08,950
+neutralization بعدين ب .. ب .. أنشفه و بامسح بميه
+
+892
+01:10:08,950 --> 01:10:12,410
+بعدين بامسح بمعقم وبالتالي هيك يعني في خطوات
+
+893
+01:10:12,410 --> 01:10:19,350
+للتخلص من أثار الحوادث فبالتاليلازم نسمع .. نسأل
+
+894
+01:10:19,350 --> 01:10:23,270
+المسة عنها علشان هي تحكيلنا كيف .. كيف نتعامل
+
+895
+01:10:23,270 --> 01:10:27,330
+معاها و نقلل من أثر الضرر اللي ممكن يلحق فينا أو
+
+896
+01:10:27,330 --> 01:10:32,210
+في زملائنا نتيجة هذا الحادث كسر أو .. انسكاب أو أي
+
+897
+01:10:32,210 --> 01:10:39,020
+إشيتمام، إيش أدوات اللي لازم نستخدمها لحتى نحقق ال
+
+898
+01:10:39,020 --> 01:10:42,900
+safety، الأمان؟ طبعا في عندي شاور، الشاور هذا و
+
+899
+01:10:42,900 --> 01:10:49,080
+مجرد ما أني أنا أشد الجزء هذا، طبعا هاي الجزء هاد،
+
+900
+01:10:49,080 --> 01:10:52,440
+مجرد ما أشده بتنزل مي، هذا طبعا إذا إيش انكب
+
+901
+01:10:52,440 --> 01:10:56,520
+علينا، أي .. على ال .. على إيدانا أو على أي جزء من
+
+902
+01:10:56,520 --> 01:11:01,510
+جسمنا، مادة، ممكن تكون حارقة أو ممكن تكون مؤذيةال
+
+903
+01:11:01,510 --> 01:11:07,130
+.. في عندي شوار .. شوار لل eye طبعا كمان هذا إذا
+
+904
+01:11:07,130 --> 01:11:11,710
+كان أي رذاذ داخل .. داخل العين لازم أنه نعرض العين
+
+905
+01:11:11,710 --> 01:11:17,550
+لهذا الشوارط ال fire blanket إذا احنا مثلا شخص
+
+906
+01:11:17,550 --> 01:11:22,690
+يعني لا سمح الله نكب عليه إشي مشتعل أو عم بينحرج
+
+907
+01:11:22,690 --> 01:11:28,650
+فلازم نلفه في بطانية عشان تمنع من أنه هذا النار
+
+908
+01:11:28,650 --> 01:11:33,510
+تبقى شاعلةو في عندي fire extinguisher برضه كمان
+
+909
+01:11:33,510 --> 01:11:42,970
+اللي هو الجرة الغاز اللي بتطفي ال .. النيران طبعا
+
+910
+01:11:42,970 --> 01:11:51,450
+في عندي إشارات لل hazards تمام؟ في عندي إشارات
+
+911
+01:11:51,450 --> 01:11:58,640
+للخطر علامات الخطر إيش يعني هذه الإشارات؟مثلا إذا
+
+912
+01:11:58,640 --> 01:12:02,560
+أنا شوفت .. ممكن أشوفها على أدوات أو على الخزانات
+
+913
+01:12:02,560 --> 01:12:07,620
+أو على الحائط موجودة طبعا يعني إذا أنا شوفت هذه
+
+914
+01:12:07,620 --> 01:12:10,840
+العلامة معناها المكان هذا في animal و ال animal
+
+915
+01:12:10,840 --> 01:12:14,160
+هذه ممكن تكون معضية أو ممرضة أو في .. يعني إحنا
+
+916
+01:12:14,160 --> 01:12:20,000
+بنجري عليها تجارب فمش لازم أقترب من هذا المكان هذا
+
+917
+01:12:20,000 --> 01:12:22,960
+يعني أن الأدوات اللي .. العلامة اللي بتكون موجودة
+
+918
+01:12:22,960 --> 01:12:28,420
+على هذه الأدوات أو ال ..الاماكن يعني الأدوات هذه
+
+919
+01:12:28,420 --> 01:12:33,520
+أو الأماكن موجود داخلها أدوات sharp يعني أدوات
+
+920
+01:12:33,520 --> 01:12:41,080
+حادة ممكن أنها تسبب جرؤ جروح أو أدى نظيف مثلا
+
+921
+01:12:41,080 --> 01:12:45,700
+للشخص إذا ما اتعامل معاها بطريقة صحيةheat hazard
+
+922
+01:12:45,700 --> 01:12:51,160
+الأدوات الساخنة أو الأجهزة اللي بتعطي حرارة لازم
+
+923
+01:12:51,160 --> 01:12:53,860
+يكون عليها هاي العلامة عشان انا أول ما أشوفها ان
+
+924
+01:12:53,860 --> 01:12:56,760
+انا ماألمساش بإيدي على طول مباشرة تاني أعرف ان هي
+
+925
+01:12:56,760 --> 01:13:02,370
+ممكن تعملي حرق فبالتاليهي الأشياء ساخنة لازم أنا
+
+926
+01:13:02,370 --> 01:13:06,150
+ألبس ال guante لسميكة قبل ألمسها أو أمسكها بال ..
+
+927
+01:13:06,150 --> 01:13:10,090
+بالملقة مثل ما حاكي هنا glassware hazard يعني هذا
+
+928
+01:13:10,090 --> 01:13:14,330
+المكان فيه أشياء زجاجية أنا مش لازم مثلا ال rough
+
+929
+01:13:14,330 --> 01:13:17,930
+العالي هذا موجود عليه هذه العلامة يعني أدوات
+
+930
+01:13:17,930 --> 01:13:21,990
+زجاجية مش لازم أنا أجي و أنا مش طايلة لل rough أجي
+
+931
+01:13:21,990 --> 01:13:28,190
+أحاول أن أنا أخد إشي منه ممكن يجع أداء زجاجية
+
+932
+01:13:28,190 --> 01:13:33,130
+تنكسر تأذنىchemical hazard طبعا في مواد كيميائية
+
+933
+01:13:33,130 --> 01:13:39,790
+بتكون موجودة ممكن مؤذية حارقة او بتسبب تهيج للجلد
+
+934
+01:13:39,790 --> 01:13:46,990
+او ممكن ان اجتمامها بيبقى مؤذى او فبالتالي بتسبب
+
+935
+01:13:46,990 --> 01:13:50,870
+خطر معين او مش لازم اخلطها مع بعض هالبعض فبالتالي
+
+936
+01:13:50,870 --> 01:13:53,610
+انا بتتعامل معها بحضر بيكون عليها هذه العلامة
+
+937
+01:13:53,610 --> 01:13:56,800
+chemical hazardهذه المواد أنا مش لازم لا أشمها ولا
+
+938
+01:13:56,800 --> 01:14:01,080
+ألامسها أو أتذوقها أو حتى أخلطها مع مواد أخرى بدون
+
+939
+01:14:01,080 --> 01:14:04,020
+تعليمات لازم أستنى ألمس أيش بتحكيلي كيف أتعامل مع
+
+940
+01:14:04,020 --> 01:14:07,440
+هذه المادة عشان أنا أظل بالسليم electrical hazard
+
+941
+01:14:07,440 --> 01:14:10,480
+طبعا هذه الماء اللي يعني على الأسلاك اللي بشوفها
+
+942
+01:14:10,480 --> 01:14:14,460
+عند الأجهزة الكهربائية انه هذا الجهاز من الخطر انه
+
+943
+01:14:14,460 --> 01:14:19,650
+يظل مشبوك بالكهربا فلازم أفصله بعد ما أخلصI face
+
+944
+01:14:19,650 --> 01:14:24,010
+.. I and face hazard طبعا ان المكان هذا بيتنبعث
+
+945
+01:14:24,010 --> 01:14:29,110
+منه رداد او ابخرة ممكن تكون مؤدية للعين او الوجه
+
+946
+01:14:29,110 --> 01:14:33,490
+فبالتالي لازم ان مثلا احط ال shield احط mask على
+
+947
+01:14:33,490 --> 01:14:38,650
+وجهي او اشي فبالتالي بدي انتبه لهذه العلامة
+
+948
+01:14:38,650 --> 01:14:41,830
+العلامة هذه بتكون موجودة على الأشياء القابلة
+
+949
+01:14:41,830 --> 01:14:46,110
+للاشتعال او اللي ممكن تحترقالعلامة هذه بتكون
+
+950
+01:14:46,110 --> 01:14:49,990
+موجودة على ال biohazard هتشوفوها على السلات اللي
+
+951
+01:14:49,990 --> 01:14:54,590
+بنطلف فيها الأدوات اللي لازم تتعقم قبل تطلف
+
+952
+01:14:54,590 --> 01:14:58,490
+حكيتلكم إنه في أدوات لازم أنا أعقمها قبل أطلفها
+
+953
+01:14:58,490 --> 01:15:03,850
+فهذه ال biohazard موجودة عليها يعني خطر بيولوجي
+
+954
+01:15:03,850 --> 01:15:08,210
+إيش خطر بيولوجي يعني فيروسات بكتيريا فطريات أشياء
+
+955
+01:15:08,210 --> 01:15:12,600
+معضية ممكن تكون موجودةهشوف .. هنشوفها على السلات و
+
+956
+01:15:12,600 --> 01:15:18,140
+على ال .. ال safety boxes و بعض الأدوات و بعض
+
+957
+01:15:18,140 --> 01:15:22,540
+الأكياس هورجيكم إياها خليكم تاخدوا انتباهكم منها
+
+958
+01:15:22,540 --> 01:15:27,100
+laser radiation طبعا يعني مثلا هذه يعني أن المواد
+
+959
+01:15:27,100 --> 01:15:31,700
+مشعة و أنا مش لازم أقترب أو ألامسها هيك ليش؟ لأنه
+
+960
+01:15:31,700 --> 01:15:37,510
+انا إذا بدي أقترب من هايال .. المادة يعني أنا أخدت
+
+961
+01:15:37,510 --> 01:15:40,870
+إشعاع بإيدي روحت لمست كتبي، الكتبي صارت مشععة،
+
+962
+01:15:40,870 --> 01:15:44,090
+لمست زميلتي، زميلتي صار في عندها إشعاع، كذا، و
+
+963
+01:15:44,090 --> 01:15:48,750
+الإشعاعات carcinogen مسببة لل cancer فبالتالي
+
+964
+01:15:48,750 --> 01:15:55,630
+مشكلة أو .. وكمان ممكن تعملي abnormal hormonal
+
+965
+01:15:55,630 --> 01:15:59,170
+profile، إيش يعني؟ يعني بتخرب كل شغل ال .. ال
+
+966
+01:15:59,170 --> 01:16:04,050
+hormonات داخل الجسمفبالتالي لازم انا انتبه لهاي
+
+967
+01:16:04,050 --> 01:16:08,070
+المواد المشعة حكينا ان تلفها حسب التعليمات في طرق
+
+968
+01:16:08,070 --> 01:16:13,770
+معينة الأداة هذه يعني برضه كمان ان في عندى إشعاع
+
+969
+01:16:13,770 --> 01:16:17,950
+هذا إشعاع laser وهو هذي إشعاع من أنواع أخرى أيضا
+
+970
+01:16:17,950 --> 01:16:22,430
+برضه كمان يعني مواد مشعة وخاطرة explosive يعني هذه
+
+971
+01:16:22,430 --> 01:16:25,590
+المادة قابلة للانفجار مش لازم انه انا مثلا اتركها
+
+972
+01:16:25,590 --> 01:16:29,490
+مفتوحةمش لازم ا .. ا .. احطها في .. ا .. اضل ارج
+
+973
+01:16:29,490 --> 01:16:33,090
+فيها مش لازم ا .. ا .. اضيف عليها مواد اتساعد من
+
+974
+01:16:33,090 --> 01:16:37,230
+انفجارها فبالتالي بدي اتعامل مع كل المواد هذه
+
+975
+01:16:37,230 --> 01:16:41,430
+تتعامل طبقا للتعليمات كيف يعني طبقا للتعليمات؟
+
+976
+01:16:41,430 --> 01:16:44,470
+يعني ال master بتحكيلكوا اضيفوا بالأول كذا بعدين
+
+977
+01:16:44,470 --> 01:16:49,050
+كذا طبعا انا ليش حاكتلك اضيفي بالأول ال .. المادة
+
+978
+01:16:49,050 --> 01:16:53,160
+الفلانية عشان لو انت عكستي ممكن يصير في مشكلةطبعا
+
+979
+01:16:53,160 --> 01:16:56,840
+فبالتالي بتنتبهوا لتعليمات لمست كتير منيحة و احنا
+
+980
+01:16:56,840 --> 01:17:01,620
+طبعا يعني الاشي مابناش ياكم تخافوا كتير لأ احنا
+
+981
+01:17:01,620 --> 01:17:05,880
+بنكون منتبهين كتير معاكم و مركزين و بنمشي خطوة
+
+982
+01:17:05,880 --> 01:17:09,020
+بخطوة و بالعكس أنتوا بتكونوا يعني موجودين على ال
+
+983
+01:17:09,020 --> 01:17:14,180
+bench حواليا انا بكون اول اشي بشتغل التجربة ب ..
+
+984
+01:17:14,180 --> 01:17:18,800
+بإيدي بورجيكم كيف التجربة بتنشغل بتشوفوها لأول مرة
+
+985
+01:17:18,800 --> 01:17:24,010
+بإيديكم .. بعينيكم sorryو بعد ما تشوفوا ال ..
+
+986
+01:17:24,010 --> 01:17:31,110
+الاشي بتروحوا تشتغلوا بإيداكم بعدين طبعا و أنتوا
+
+987
+01:17:31,110 --> 01:17:34,890
+بتشتغلوا أنا بكون متبعة معاكم كمان يعني ما بسيبكم
+
+988
+01:17:34,890 --> 01:17:37,790
+تشتغلوا هيك لحالكم لأ يعني أنتوا بتكونوا شوفتي
+
+989
+01:17:37,790 --> 01:17:41,250
+التجربة لأول مرة و أنا بشتغلها بعد ما أكون أنا
+
+990
+01:17:41,250 --> 01:17:45,930
+شرحتهايعني بتكون التجربة مرت عليكي للمرة التانية و
+
+991
+01:17:45,930 --> 01:17:49,410
+بعدين لما تيجي تشتغليها بإيداكي أنا بمشي معاكم
+
+992
+01:17:49,410 --> 01:17:52,810
+خطوة خطوة بقولكم يلا خطوة رقم واحد ضيفي المادة
+
+993
+01:17:52,810 --> 01:17:56,010
+كذا، خطوة رقم اتنين ضيفي المادة كذا و طبعا
+
+994
+01:17:56,010 --> 01:17:59,490
+مابننطقلش للخطوة رقم اتنين إلا لما يكون الكل أضاف
+
+995
+01:17:59,490 --> 01:18:02,710
+و الكل اشتغل و الكل ماشي علشان في النهاية نكون
+
+996
+01:18:02,710 --> 01:18:07,590
+كلنا ماشيين زي بعض و كلنا بنشتغل مع بعضو الكل أخد
+
+997
+01:18:07,590 --> 01:18:10,910
+.. منتبه و ماخد التعليمات أول بأول لكن لو تركت
+
+998
+01:18:10,910 --> 01:18:14,410
+مثلا ناس خلص و ناس لسه بدين و ناس نسلم من نص الشغل
+
+999
+01:18:14,410 --> 01:18:19,050
+هيصير في فوضى، هيصير في ناس مش صامعة تعليمات كويس،
+
+1000
+01:18:19,050 --> 01:18:22,730
+في ناس ملتزماتش كويس فلأ خطوة بخطوة و مع بعض و
+
+1001
+01:18:22,730 --> 01:18:27,230
+متابعين أول بأول علشان في النهاية تكونوا بتشتغلوا
+
+1002
+01:18:27,230 --> 01:18:31,730
+صح و بتتعلموا صح و مركزين معايا لكل كلمة بحكيها،
+
+1003
+01:18:31,730 --> 01:18:38,450
+كل كلمة بحكيها بتكون مهمةفي عندي أشياء أخرى من ال
+
+1004
+01:18:38,450 --> 01:18:47,090
+.. الرموز هي inflammable يعني مادة قابلة للاشتعال
+
+1005
+01:18:47,090 --> 01:18:52,150
+تمام هذه مادة بتسبب تهيج للجسم مادة حارقة بتسبب
+
+1006
+01:18:52,150 --> 01:18:55,610
+حروق ال bio hazard عرفنا إيش يعني ال explosion
+
+1007
+01:18:55,610 --> 01:19:00,870
+قابلة للانفجار يعرفنا إيش يعني ال .. هذه مادة سامة
+
+1008
+01:19:00,870 --> 01:19:06,580
+هذه مادة قابلة للأكسدة والاشتعالو هذه طبعا المادة
+
+1009
+01:19:06,580 --> 01:19:10,640
+ده إذا أنا لازم أتلفها بطريقة معينة مش لازم أوصي
+
+1010
+01:19:10,640 --> 01:19:15,280
+.. يعني أكبها كب بالحوض أو بالسلة لإنها لو وصلت
+
+1011
+01:19:15,280 --> 01:19:19,500
+للطربة أو للنباتات أو للبحار لل .. ممكن تأذي
+
+1012
+01:19:19,500 --> 01:19:23,200
+النباتات أو تأذي ثروة السمكية أو تأذي الحيوانات
+
+1013
+01:19:23,200 --> 01:19:28,600
+فبالتالي بتسبب خطر على البيئة تعمل تلوث بيئي فإذا
+
+1014
+01:19:28,600 --> 01:19:32,000
+وجدت هذه المادة .. العلامة على أي مادة فأنا لازم
+
+1015
+01:19:32,000 --> 01:19:36,380
+نتلفها بطريقة صحيحةو هذه معناها مادة مشعة و حكينا
+
+1016
+01:19:36,380 --> 01:19:41,580
+إيش يعني، هيك بنكون خلصنا مختصر ال lab safety و
+
+1017
+01:19:41,580 --> 01:19:44,620
+عرفنا إيش يعني ال medical laboratory، هذا عنوان
+
+1018
+01:19:44,620 --> 01:19:52,250
+محاضرتناال .. المادة .. النظرية كان و راح ان شاء
+
+1019
+01:19:52,250 --> 01:19:57,170
+الله نحكي عن ال lab safety أيضا في العمل بمعلومات
+
+1020
+01:19:57,170 --> 01:20:01,730
+أخرى تمام؟ هتشوفوا فيها تكرار تمام؟ ولكن هنضيف بعض
+
+1021
+01:20:01,730 --> 01:20:05,050
+المعلومات الجديدة و ان شاء الله تكون بسيطة وسهلة
+
+1022
+01:20:05,050 --> 01:20:08,150
+عليكم يعطيكم ألف عافية و شكرا لكم
+
diff --git a/PL9fwy3NUQKwY9jl8xw-bWkVZkWcGGyspd/RK30qovrvdg.srt b/PL9fwy3NUQKwY9jl8xw-bWkVZkWcGGyspd/RK30qovrvdg.srt
new file mode 100644
index 0000000000000000000000000000000000000000..60ab17de9d152095d62219c82cc359ecbc6dac64
--- /dev/null
+++ b/PL9fwy3NUQKwY9jl8xw-bWkVZkWcGGyspd/RK30qovrvdg.srt
@@ -0,0 +1,2129 @@
+1
+00:00:06,980 --> 00:00:10,700
+السلام عليكم ورحمة الله وبركاته، طالبات العزيزات
+
+2
+00:00:10,700 --> 00:00:14,680
+اليوم إن شاء الله رح نتحدث عن الـ essential lab
+
+3
+00:00:14,680 --> 00:00:20,360
+instruments، تمام؟ رح نتطرق لبعض أجهزة المختبر و
+
+4
+00:00:20,360 --> 00:00:27,450
+نتعرف على مبدأ عملها، طريقة عملها، و استخدامها بما
+
+5
+00:00:27,450 --> 00:00:34,470
+أنه كان المساق كله تعريفي بالمختبر، وأدواته، و
+
+6
+00:00:34,470 --> 00:00:40,190
+أجهزته، ويعني كان في بعض الفحوصات الأساسية اللي
+
+7
+00:00:40,190 --> 00:00:44,650
+إحنا تعلمناها، وكنا بنستخدم بعض الأجهزة، واتعرفنا
+
+8
+00:00:44,650 --> 00:00:49,390
+على centrifuge ومبدأ عمله، واستخدامه، واتعرفنا على
+
+9
+00:00:49,390 --> 00:00:54,570
+spectrophotometer والـ microscope. كمان اليوم راح
+
+10
+00:00:54,570 --> 00:00:58,600
+نذكر بعض الأجهزة الأخرى، مثل الـ balances.
+
+11
+00:00:58,600 --> 00:01:04,880
+طبعاً الموازين، الـ balances، هي عبارة عن essential
+
+12
+00:01:04,880 --> 00:01:11,680
+laboratory instruments، أجهزة أساسية في المختبر، و
+
+13
+00:01:11,680 --> 00:01:15,760
+طبعاً هي widely used for weighing of various
+
+14
+00:01:15,760 --> 00:01:20,400
+substances. طبعاً إحنا بنستخدمها لوزن المواد سواء
+
+15
+00:01:20,400 --> 00:01:24,660
+كانت هذه المواد powder, crystals، المهم مواد صلبة
+
+16
+00:01:24,660 --> 00:01:29,720
+يتم وزنها باستخدام الـ balances. تمام، وطبعاً لحتى
+
+17
+00:01:29,720 --> 00:01:35,320
+نحضر reagents وصبغات، culture media اللي هي المادة
+
+18
+00:01:35,320 --> 00:01:39,040
+المغذية لتنمية البكتيريا، والتعرف عليها، وتشخيص
+
+19
+00:01:39,040 --> 00:01:44,500
+الأمراض البكتيرية، وحتى الفطريات، بيلزمنا إنّي لحتى
+
+20
+00:01:44,500 --> 00:01:48,600
+أحضّر وسط ينمو عليه البكتيريا أو الفطر، أني أوزن
+
+21
+00:01:48,600 --> 00:01:54,940
+من هذه المادة المغذية، وأذوبها في this solvent.
+
+22
+00:01:54,940 --> 00:02:00,280
+تمام، إذاً الـ balances بتستخدم for weighing
+
+23
+00:02:00,280 --> 00:02:05,020
+accurately and precisely، طبعاً within a needed
+
+24
+00:02:05,020 --> 00:02:10,800
+range. طبعاً بيكون في الميزان range بيتحمل وزن من
+
+25
+00:02:10,800 --> 00:02:14,800
+إلى، وطبعاً أنا بستخدمه طبقاً لهذا الـ range. نفس فكرة
+
+26
+00:02:14,800 --> 00:02:17,420
+الـ by bit، يعني أنا بستخدم الـ by bit علشان أنقل
+
+27
+00:02:17,420 --> 00:02:22,850
+volume، وكل by bit بيكون لها range خاص فيها. تمام، إذاً
+
+28
+00:02:22,850 --> 00:02:29,590
+الـ balances هي عبارة عن موازين أو ميزان يستخدم في
+
+29
+00:02:29,590 --> 00:02:33,350
+وزن المواد الصلبة powder أو crystal، سواء لو كنت
+
+30
+00:02:33,350 --> 00:02:40,730
+بدي أحضّر محلول reagents، صبغات، buffer، محلول ملحي،
+
+31
+00:02:40,730 --> 00:02:45,090
+سواء كان بدي أحضّر cultural media، وطبعاً في عندي
+
+32
+00:02:45,090 --> 00:02:49,010
+نوعين من هذه الموازين: الـ rough balances أو الـ
+
+33
+00:02:49,010 --> 00:02:52,870
+mechanical balances، والـ analytical balances. تمام.
+
+34
+00:02:52,870 --> 00:02:57,050
+نيجي نتعرف على الـ rough balances، هي عبارة عن طبعاً
+
+35
+00:02:57,050 --> 00:03:03,130
+mechanical، يعني يتم الوزن والضبط يدوياً، ويحتوي على
+
+36
+00:03:03,130 --> 00:03:06,910
+الـ... هذا الميزان على sliding scale. هنا بيكون في
+
+37
+00:03:06,910 --> 00:03:10,630
+تدريج، وبيكون في عندي قطع بتنزلق على هذا التدريج،
+
+38
+00:03:10,630 --> 00:03:15,690
+بتتحرك يميناً ويساراً، تمام؟ مجرد ما أنا أضع المادة
+
+39
+00:03:15,690 --> 00:03:20,930
+المراد وزنها، ببدأ أحرك هذه القطع اللي بتنزلق على
+
+40
+00:03:20,930 --> 00:03:26,990
+الـ scale، على التدريج، لحد ما المؤشر هنا يصل لمنتصف،
+
+41
+00:03:26,990 --> 00:03:31,390
+يعني إنه خلاص صار في balance. تمام، صار في اتزان في
+
+42
+00:03:31,390 --> 00:03:36,890
+الـ... هذا الميزان، وبقى آخذ... بعرف اللي يعني لحد
+
+43
+00:03:36,890 --> 00:03:39,970
+ما وين وصلت القطعة اللي انزلقت، بقى آخذ القراءة
+
+44
+00:03:39,970 --> 00:03:43,410
+على التدريج، وبعرف إنه هذا الوزن. طبعاً مثل ما أنتم
+
+45
+00:03:43,410 --> 00:03:47,270
+شايفين، هذا الـ rough balances مش كتير sensitive، يعني
+
+46
+00:03:47,270 --> 00:03:50,730
+ما بيقدر يفرّق لي ما بين الـ... الـ... يعني الفروق
+
+47
+00:03:50,730 --> 00:03:56,710
+البسيطة في الـ... في الأوزان، وأيضاً يعني بيستخدم لـ
+
+48
+00:03:56,710 --> 00:04:00,030
+الـ large amount، يعني أنا لو بدي أوزن كميات أو
+
+49
+00:04:00,030 --> 00:04:04,190
+أوزان عالية أو أوزان كبيرة، ولما بدي يكون عندي هذه
+
+50
+00:04:04,190 --> 00:04:10,420
+الأوزان مش مطلوبة يعني high degree of accuracy مش...
+
+51
+00:04:10,420 --> 00:04:16,860
+مش متطلب مني إنه يكون عندي يعني درجة دقة وصحة
+
+52
+00:04:16,860 --> 00:04:20,960
+عالية، فممكن أنا أستخدم هذه الـ rough balances. طبعاً
+
+53
+00:04:20,960 --> 00:04:24,560
+هذه الـ rough balances ما بتحتاج main electricity،
+
+54
+00:04:24,560 --> 00:04:27,160
+ما بتحتاج أني أشبكها بالكهرباء أو أن يكون في
+
+55
+00:04:27,160 --> 00:04:34,320
+عندها battery... بطارية. طبعاً حكينا إنها هي less
+
+56
+00:04:34,320 --> 00:04:40,420
+expensive، مش غالية، وكمان مش sensitive، طبعاً إلا إنه
+
+57
+00:04:40,420 --> 00:04:45,700
+في بعض الأنواع ممكن يعني يفرّق ما بين الـ... الـ...
+
+58
+00:04:45,700 --> 00:04:50,560
+يعني الموازين، وممكن يوزن لي لحد point one gram of
+
+59
+00:04:50,560 --> 00:04:55,900
+substance. تمام؟ هذا الـ... ما يتعلق بالـ mechanical
+
+60
+00:04:55,900 --> 00:04:59,000
+balance. الـ analytical balance لأ، هو دقيق جداً، هو
+
+61
+00:04:59,000 --> 00:05:03,420
+اللي اشتغلنا عليه لما كنا نوزن الماء، ونعمل
+
+62
+00:05:03,420 --> 00:05:07,960
+calibration للـ pipet. طبعاً هو دقيق جداً، وطبعاً
+
+63
+00:05:07,960 --> 00:05:13,400
+بيحتاج، بما أنه هو analytical وelectronic، لازم أنا
+
+64
+00:05:13,400 --> 00:05:18,610
+يكون موصول بالكهرباء، أو ممكن يكون أو بيشتغل على
+
+65
+00:05:18,610 --> 00:05:22,350
+بطارية، needs main electricity أو battery، وبرضه
+
+66
+00:05:22,350 --> 00:05:26,270
+كمان إنه هو highly sensitive، حساس جداً، ممكن يفرّق لي
+
+67
+00:05:26,270 --> 00:05:31,190
+بين الأوزان بدقة، يعني لو point واحد فرق بالوزن،
+
+68
+00:05:31,190 --> 00:05:37,010
+ممكن يوضح لي إياه، وطبعاً high accurate and precise
+
+69
+00:05:37,010 --> 00:05:42,770
+instrument، وطبعاً بيوزن بمبدأ الـ electro...
+
+70
+00:05:42,770 --> 00:05:46,980
+الـ electromagnetic force. تمام؟ يعني أنا مش محتاجة
+
+71
+00:05:46,980 --> 00:05:50,560
+أني أعاير شيء بمقابل الوزن، علشان أ... يعني ما
+
+72
+00:05:50,560 --> 00:05:53,780
+...مش... مش محتاجة أحصل على المكاييل المعدنية، وأ
+
+73
+00:05:53,780 --> 00:05:57,840
+أحطهم، وأكيل، أو أني أحرك على sliding scale لحد ما
+
+74
+00:05:57,840 --> 00:06:01,760
+يتزن المؤشر إلى نص الميزان، زي ما بنعمل بالـ...
+
+75
+00:06:01,760 --> 00:06:07,630
+الـ... الـ... الـ... الـ rough balances. طبعاً مش محتاجة
+
+76
+00:06:07,630 --> 00:06:13,310
+لهذا الشيء. طبعاً الـ rough balances ممكن يكون بـ one
+
+77
+00:06:13,310 --> 00:06:19,850
+pan، يعني إله دفة واحدة، أو double pan، طبعاً دفتان. طيب،
+
+78
+00:06:19,850 --> 00:06:24,190
+فبالتالي إحنا مش محتاجين إنّا يعني شيء أعاير فيه،
+
+79
+00:06:24,190 --> 00:06:28,030
+sliding scale أو أوزان معيارية، إنما بطريقة...
+
+80
+00:06:28,030 --> 00:06:33,010
+بمبدأ عمل الـ electromagnetic force، ممكن يعني...
+
+81
+00:06:33,010 --> 00:06:37,510
+يعني كم بـ... يعني الـ... المادة ينشأ عنها
+
+82
+00:06:37,510 --> 00:06:42,650
+magnetic force، بيعطيني وزن لها، تمام؟ طيب،
+
+83
+00:06:42,650 --> 00:06:46,710
+بيستخدم لما أنا بدي أوزن أوزان صغيرة جداً، ولما
+
+84
+00:06:46,710 --> 00:06:53,110
+بيكون في عندي الـ high accuracy يعني مطلوبة، تمام؟
+
+85
+00:06:53,110 --> 00:06:56,910
+when great accuracy is required. على عكس اللي هي الـ
+
+86
+00:06:56,910 --> 00:07:01,550
+rough balances. طيب، أنا كيف بدي أستخدم هذا الميزان،
+
+87
+00:07:01,550 --> 00:07:06,050
+وكيف أنا بدي أعمله care، أهتم فيه؟ طبعاً الـ balance
+
+88
+00:07:06,050 --> 00:07:10,190
+هو عبارة عن جهاز، instrument، حساس، delicate
+
+89
+00:07:10,190 --> 00:07:14,630
+instrument، وبيحتاج أني يعني أتبع التعليمات
+
+90
+00:07:14,630 --> 00:07:18,810
+أثناء الاستخدام، أستخدمه بشكل صحيح، correct use، و
+
+91
+00:07:18,810 --> 00:07:24,890
+أعتني فيه. فطبعاً أول حاجة لازم قبل البدء بتشغيل أي
+
+92
+00:07:24,890 --> 00:07:31,170
+جهاز أو باستخدام أي جهاز، هذا يعني ينطبق على الكل، من
+
+93
+00:07:31,170 --> 00:07:34,810
+ضمنهم الـ balances، أني أقرأ الـ manufacturer
+
+94
+00:07:34,810 --> 00:07:39,310
+instructions. طبعاً بيجي catalog مع كل جهاز من الشركة
+
+95
+00:07:39,310 --> 00:07:43,830
+المصنعة، وفي تعليمات كيفية الاستخدام، والدّبط، و
+
+96
+00:07:43,830 --> 00:07:50,410
+المعايرة، وكيفية التشغيل، ولو في خلل... حتى يعني في
+
+97
+00:07:50,410 --> 00:07:53,600
+خلل معيّن، ممكن كيف أتجنّبه. كل هذه الشغلات بيكون
+
+98
+00:07:53,600 --> 00:07:57,160
+موجودة في الـ catalog، فأنا لازم أقرأ هذا الـ
+
+99
+00:07:57,160 --> 00:07:59,820
+catalog أو الـ instructions، حتى كمان التركيب، طريقة
+
+100
+00:07:59,820 --> 00:08:02,960
+التركيب إذا كان إجا الجهاز عبارة عن قطع، وأنا بدي
+
+101
+00:08:02,960 --> 00:08:06,100
+أركّبهم، برضه كمان بيكون موجود في الـ manufacturer
+
+102
+00:08:06,100 --> 00:08:10,350
+instructions and catalog. Always handle a balance
+
+103
+00:08:10,350 --> 00:08:13,010
+with care. طبعاً لازم أنا يعني أتعامل مع الـ
+
+104
+00:08:13,010 --> 00:08:19,870
+balance وأستخدمه باهتمام، برعاية جداً، وطبعاً
+
+105
+00:08:19,870 --> 00:08:23,250
+position the balance on a firm bench away from
+
+106
+00:08:23,250 --> 00:08:28,310
+vibration. طبعاً أنا بحاول أني أحط الميزان على
+
+107
+00:08:28,310 --> 00:08:33,090
+بنش ثابت، بعيد عن الاهتزازات، يعني ما يكون تحت مروحة
+
+108
+00:08:33,090 --> 00:08:36,370
+أو مقابل يعني...
+
+109
+00:08:37,830 --> 00:08:42,250
+الـ تكييف، مثلاً إذا إجا يعني طيار الهواء بيخبط فيه،
+
+110
+00:08:42,250 --> 00:08:45,810
+هذا هيأثّر، ويكون بعيد مثلاً عن التلاجة، أنتم
+
+111
+00:08:45,810 --> 00:08:51,050
+بتعرفوا مثلاً تلاجة، computer، أجهزة فيها hardware
+
+112
+00:08:51,050 --> 00:08:55,070
+و software، ممكن إنها أيضاً تعمل ذبذبات و
+
+113
+00:08:55,070 --> 00:08:59,530
+اهتزازات، فهذا رح يأثّر. أيضاً ببعده عن الهواء، وضوء
+
+114
+00:08:59,530 --> 00:09:04,310
+الشمس، ببعده عن الرطوبة، أو يصير يعني...
+
+115
+00:09:04,310 --> 00:09:07,930
+كمان حتى ببعده عن الـ tap water، حتى ما يجي عليه مية
+
+116
+00:09:07,930 --> 00:09:12,070
+ويصدأ. فهذه الشغلات كمان يعني تدخل من ضمن الـ care،
+
+117
+00:09:12,070 --> 00:09:16,190
+وإنّي أنا كيف أحافظ على الجهاز. Before starting to
+
+118
+00:09:16,190 --> 00:09:20,010
+weigh, zero the balance. طبعاً قبل استخدام هذا
+
+119
+00:09:20,010 --> 00:09:24,810
+الميزان، لازم أنا أصفّره، وإذا كنت بأستخدم beam
+
+120
+00:09:24,810 --> 00:09:27,610
+balance، لازم أتأكّد على الـ position للـ beam، إيش
+
+121
+00:09:27,610 --> 00:09:31,170
+يعني الـ beam balance؟ شوفتوا الميزان اللي بيكون
+
+122
+00:09:31,170 --> 00:09:35,370
+على رمز المحكمة أو العدالة، اللي هو عبارة عن دفتين
+
+123
+00:09:35,370 --> 00:09:41,400
+معلقتين، بجانبيْ zero فيهم في... يعني زي حامل بالنص
+
+124
+00:09:41,400 --> 00:09:45,800
+بالمركز. طبعاً أنا لازم أتأكّد من إنه هذول الـ... الـ...
+
+125
+00:09:45,800 --> 00:09:49,660
+...الحامل اللي بالمركز، والدفتين، موضوع... يعني الـ
+
+126
+00:09:49,660 --> 00:09:53,740
+position تبعهم كتير منيح، مضبوط، علشان أنا أستخدمهم.
+
+127
+00:09:53,740 --> 00:09:57,240
+تمام؟ هو بيكون بـ... بيكون محتاج لأوزان معيارية
+
+128
+00:09:57,240 --> 00:10:01,220
+في هذه الحالة. طبعاً إذا أنا بدي أوزن chemicals على
+
+129
+00:10:01,220 --> 00:10:06,260
+درجة حرارة الغرفة، لازم أحط المادة المراد وزنها في
+
+130
+00:10:06,260 --> 00:10:12,360
+container، بـ... cup أو small beaker، طبعاً علشان...
+
+131
+00:10:12,360 --> 00:10:15,000
+طبعاً أنتم لما كنا نوزن، يعني أكيد لازم
+
+132
+00:10:15,000 --> 00:10:19,180
+تستخدموا cup فارغ، لازم تستخدموا beaker أو... كنا
+
+133
+00:10:19,180 --> 00:10:25,400
+أحياناً نتوزن على ورقة. في النهاية إنه أنا طبعاً ما...
+
+134
+00:10:25,400 --> 00:10:28,280
+يعني ما أحط الـ... الـ chemical، never put the
+
+135
+00:10:28,280 --> 00:10:31,200
+chemical directly on the balance، فعلاً يعني على دفة
+
+136
+00:10:31,200 --> 00:10:35,460
+الـ... الميزان، ما أحط المادة الكيميائية الـ... الـ...
+
+137
+00:10:35,460 --> 00:10:40,880
+المراد وزنها مباشرة، مهما كان نوع هذه المادة، ممنوع
+
+138
+00:10:40,880 --> 00:10:44,500
+أني أحطها، لأنها ممكن إنها... تلّف الجهاز. When
+
+139
+00:10:44,500 --> 00:10:48,200
+adding or removing a chemical, remove the container
+
+140
+00:10:48,200 --> 00:10:52,740
+to avoid spilling and chemical on the balance. طبعاً
+
+141
+00:10:52,740 --> 00:10:56,300
+لما أنا بدي أوزن، لازم أحط المادة المراد وزنها في
+
+142
+00:10:56,300 --> 00:10:58,840
+هذا الـ container، ولما بدي أرفع، برفع الـ container
+
+143
+00:10:58,840 --> 00:11:03,280
+كله، علشان في النهاية أنا بدي أمنع انسكاب أي مادة
+
+144
+00:11:03,280 --> 00:11:06,900
+كيميائية، أو يعني إنها تنكب على الميزان، ممكن إنها
+
+145
+00:11:06,900 --> 00:11:10,020
+تتسرب إلى الداخل، تعمل انسدادات، أو تأثّر على الجهاز
+
+146
+00:11:10,020 --> 00:11:16,110
+من جوة، أو حتى تلوّث الجهاز أو تُخرّبه. Always use
+
+147
+00:11:16,110 --> 00:11:19,590
+forceps to add or remove weight. طبعاً أنا إذا بدي
+
+148
+00:11:19,590 --> 00:11:24,130
+أنقل مادة لحتى أوزنها، أو يعني شيء، لازم أنا أستخدم
+
+149
+00:11:24,130 --> 00:11:27,050
+الـ forceps، بالملقط، أو حتى أستخدم الـ spatula اللي
+
+150
+00:11:27,050 --> 00:11:30,530
+هي المعلقة المعدنية الصغيرة المخصصة لنقل المواد
+
+151
+00:11:30,530 --> 00:11:34,980
+المراد وزنها. طيب، protect the weight from dust، إنّي
+
+152
+00:11:34,980 --> 00:11:40,940
+أنا أحاول أحافظ على الجهاز من الغبار، والرطوبة، وإنه
+
+153
+00:11:40,940 --> 00:11:45,980
+ما ينمو عليه الفطريات،
+
+154
+00:11:45,980 --> 00:11:51,200
+ومثلاً بغطّي الجهاز إذا كان إله cover، طبعاً، والأوزان
+
+155
+00:11:51,200 --> 00:11:54,800
+اللي بوزنها، بحاول إنّي كمان... يعني اللي ما بدي
+
+156
+00:11:54,800 --> 00:11:57,620
+أوزنها، أستخدمها، أغطّيها، إذا ما بدي أستخدمها مباشرة،
+
+157
+00:11:57,620 --> 00:12:01,540
+لحتى برضه كمان أحافظ عليها، ممكن أي غبار، رطوبة، أو
+
+158
+00:12:01,540 --> 00:12:06,480
+حتى يعني تعفّن فيها، يأثّر على وزنها، وجودتها، ويأثّر
+
+159
+00:12:06,480 --> 00:12:08,540
+على المحلول اللي أنا بدي أحضّره باستخدام هذه
+
+160
+00:12:08,540 --> 00:12:11,920
+الأوزان. Use a small brush to remove any chemical
+
+161
+00:12:11,920 --> 00:12:16,580
+فهي بيكون عندي فرشاة مخصصة لتنظيف الـ pan،
+
+162
+00:12:16,580 --> 00:12:21,600
+للميزان، ودفة الميزان، لحتى ما يضلّ عالق عليه أي
+
+163
+00:12:21,600 --> 00:12:26,060
+أجزاء من المادة المراد وزنها، فبالتالي هيك أنا بضلّ
+
+164
+00:12:26,060 --> 00:12:30,810
+محافظة على الجهاز. الـ oven، الـ ovens اللي هي الأفران،
+
+165
+00:12:30,810 --> 00:12:36,750
+أيضاً بنستخدم أفران في المختبرات. طبعاً في عندي hot
+
+166
+00:12:36,750 --> 00:12:42,070
+air oven، اللي هو الـ... الـ oven اللي بي... بي طبعاً
+
+167
+00:12:42,070 --> 00:12:45,630
+فرن، بس مش بيكون في حرارة، يعني في نار مولعة، أو في
+
+168
+00:12:45,630 --> 00:12:49,830
+لهب، أو في شيء، لأ، هو بي... يعني بيبدأ... بيشتغل
+
+169
+00:12:49,830 --> 00:12:53,310
+بمبدأ عمل تدوير الهواء الساخن، يعني في هواء ساخن
+
+170
+00:12:53,310 --> 00:12:58,760
+بيطلع في الجهاز، بي... بيتم دورانه في الجهاز، فبي...
+
+171
+00:12:58,760 --> 00:13:02,640
+بيسخّن الأشياء اللي... اللي موجودة في الجهاز. طبعاً
+
+172
+00:13:02,640 --> 00:13:06,760
+بيستخدم الـ oven
+
+223
+00:16:42,570 --> 00:16:45,590
+أستخدم بدل ما أستخدم الـ water bath، أستخدم الـ heat
+
+224
+00:16:45,590 --> 00:16:50,170
+block تمام؟ يعني هو عبارة عن block معدني بيسخن عن
+
+225
+00:16:50,170 --> 00:16:53,210
+درجة حرارة معينة و بيظل ثابت درجة الحرارة، و برضه
+
+226
+00:16:53,210 --> 00:16:55,630
+نفس الشيء بيعملي incubation لأن أنا بيبقى على درجة
+
+227
+00:16:55,630 --> 00:16:59,870
+الحرارة اللي بدي إياها، يعني أنا بختارها هذه الدرجة. طيب
+
+228
+00:16:59,870 --> 00:17:03,690
+فبالتالي هو عبارة عن dry bath، إذا هو مش water
+
+229
+00:17:03,690 --> 00:17:06,470
+bath، لأ، dry bath لأنه زي ما أنتم شايفين مش water
+
+230
+00:17:06,470 --> 00:17:09,970
+في الموضوع، و هو block معدني بيسخن و بيبرد حسب درجة
+
+231
+00:17:09,970 --> 00:17:14,070
+الحرارة اللي أنا بدي إياها. تمام، الـ chemical test
+
+232
+00:17:14,070 --> 00:17:17,850
+التفاعلات الكيميائية، react test at specific
+
+233
+00:17:17,850 --> 00:17:21,730
+temperature. طبعا كل تفاعل كيميائي له درجة حرارة
+
+234
+00:17:21,730 --> 00:17:26,470
+خاصة بيشتغل عليها، و بيكون أفضل reaction أو أفضل
+
+235
+00:17:26,470 --> 00:17:31,670
+نتيجة على هاي درجة الحرارة. Many tests react at room
+
+236
+00:17:31,670 --> 00:17:35,990
+temperature. في تفاعلات احنا كنا بيمكن نعمل لهم
+
+237
+00:17:35,990 --> 00:17:40,210
+incubation على درجة حرارة الغرفة، من 18 لـ 22 مثلا،
+
+238
+00:17:40,210 --> 00:17:46,310
+زي الـ ... فحص الـ hemoglobin و فحص الـ glucose، كنا
+
+239
+00:17:46,310 --> 00:17:50,940
+نستنى 10 دقائق في درجة حرارة الغرفة. طيب، و لو أنا
+
+240
+00:17:50,940 --> 00:17:55,200
+بدي ... ممكن بقى أحيانا التفاعلات بتكون بتحتاج درجة
+
+241
+00:17:55,200 --> 00:17:59,340
+حرارة 37. أيضا الـ hemoglobin و الـ glucose كان ممكن
+
+242
+00:17:59,340 --> 00:18:01,940
+أن أنا أحطهم على ... في الـ water bath على درجة حرارة
+
+243
+00:18:01,940 --> 00:18:08,220
+37 و أستنى خمس دقائق مش عشرة. طبعا، فإذا الـ ... الـ
+
+244
+00:18:08,220 --> 00:18:12,220
+incubation time بيختلف من درجة حرارة إلى أخرى، و
+
+245
+00:18:12,220 --> 00:18:16,260
+أيضا يعني كل تفاعل له الـ best incubation
+
+246
+00:18:16,260 --> 00:18:21,360
+temperature. تمام؟ فأنا بضبط الـ ... الـ water bath
+
+247
+00:18:21,360 --> 00:18:25,340
+على حسب الـ ... الدرجة الحرارة الأفضل للتفاعل اللي
+
+248
+00:18:25,340 --> 00:18:31,540
+أنا عم بشتغله. Use and care of water bath. كيف أنا
+
+249
+00:18:31,540 --> 00:18:35,700
+أستخدم جهاز الـ water bath و كيف أهتم فيه. طبعا زي
+
+250
+00:18:35,700 --> 00:18:38,860
+ما أنتم شايفين، Read manufacturing instruction. هذي
+
+251
+00:18:38,860 --> 00:18:43,140
+هتشوفوها في كل الأجهزة كأول بند، أن أنا لازم أقرأ
+
+252
+00:18:43,140 --> 00:18:46,420
+تعليمات الجهة المصنعة، و الـ catalog الخاص بالجهاز
+
+253
+00:18:46,420 --> 00:18:50,120
+لحتى أفهم كل شيء بيتعلق فيه، تركيبه، تشغيله،
+
+254
+00:18:50,120 --> 00:18:53,820
+calibration لإله، إذا بيحتاج calibration، و نصائح
+
+255
+00:18:53,820 --> 00:18:58,440
+أخرى. Fill the bath and maintain its level with
+
+256
+00:18:58,440 --> 00:19:02,260
+distilled water; if unavailable, use boiled water.
+
+257
+00:19:02,260 --> 00:19:07,320
+طبعا أنا بدي أملأ هذا الجهاز بالـ distilled water،
+
+258
+00:19:07,320 --> 00:19:10,300
+يفضل distilled water عشان ما ... لو أنا بدي أستخدم الـ
+
+259
+00:19:10,300 --> 00:19:15,690
+tap water، الـ tap water فيها أملاح، و الـ ... ممكن ترسب
+
+260
+00:19:15,690 --> 00:19:20,750
+على جدران الـ water bath و تؤدي إلى تلفه. فبالتالي
+
+261
+00:19:20,750 --> 00:19:23,150
+يفضل أن أنا أستخدم distilled water. إذا ما ...
+
+262
+00:19:23,150 --> 00:19:25,750
+ماعندي distilled water متوفر، ممكن أستخدم الماء
+
+263
+00:19:25,750 --> 00:19:31,000
+المغلي، اللي هو الـ boiled water. طيب، و طبعا ليش حكينا
+
+264
+00:19:31,000 --> 00:19:34,920
+This is necessary to minimize salt
+
+265
+00:19:34,920 --> 00:19:40,100
+deposition on the heater. عشان ما يقلل ترسيب الأملاح
+
+266
+00:19:40,100 --> 00:19:44,680
+في الجهاز، to minimize the growth of microorganisms.
+
+267
+00:19:44,680 --> 00:19:48,940
+طبعا حتى الميّه ما تعفن و ما تخضرّ، و ما يصير فيها زي
+
+268
+00:19:48,940 --> 00:19:54,520
+الخضار، عفن أو زي هيك، زي الـ ... هي التحاليل بيكون فيها
+
+269
+00:19:54,520 --> 00:20:00,040
+لازم أنا أضيف مادة معقّمة، اللي هي disinfectant و
+
+270
+00:20:00,040 --> 00:20:04,220
+bactericidal agent. يعني ماء عامل قاتل للبكتيريا،
+
+271
+00:20:04,220 --> 00:20:08,100
+اللي هو الـ mercellate. تمام؟ بضيف للماء اللي أنا
+
+272
+00:20:08,100 --> 00:20:12,660
+ضفته في الـ water bath، الـ mercellate. طبعا لكل مثلا
+
+273
+00:20:12,660 --> 00:20:18,420
+ألف milli، يعني لكل one liter مثلا من الماء اللي
+
+274
+00:20:18,420 --> 00:20:27,010
+أنا ضفته، بحط one ml لكل ألف ml. تمام، بحط one ml من
+
+275
+00:20:27,010 --> 00:20:32,030
+الـ mercellate. يعني الـ dilution واحد لألف. Before
+
+276
+00:20:32,030 --> 00:20:35,690
+incubating samples, check the temperature. جاب
+
+277
+00:20:35,690 --> 00:20:40,250
+اللي أعمل incubation للعينات، لازم أعمل check على
+
+278
+00:20:40,250 --> 00:20:44,330
+الـ temperature، أشيك عليها، وإذا كان محتاجة أن أنا
+
+279
+00:20:44,330 --> 00:20:51,080
+مثلا ... يعني أطبطّها على درجة حرارة أعلى أو أقل، و أيضا
+
+280
+00:20:51,080 --> 00:20:53,560
+ممكن أنا أتأكد من درجة الحرارة باستخدام
+
+281
+00:20:53,560 --> 00:20:59,040
+thermometer. طبعا لازم يكون مستوى الماء، الـ level
+
+282
+00:20:59,040 --> 00:21:02,440
+of water is above the level of whatever is
+
+283
+00:21:02,440 --> 00:21:06,800
+being incubated. يعني معنى هذه الجملة أنه لازم يكون
+
+284
+00:21:06,800 --> 00:21:11,800
+مستوى الماء يغمر الأنابيب المراد مثلا الـ incubation
+
+285
+00:21:11,800 --> 00:21:15,520
+لها. يعني إذا كان أنا بستخدم أنابيب كبيرة، و
+
+286
+00:21:15,520 --> 00:21:18,380
+المحلول اللي فيها عالي، بدي أزوّد نسبة الماء عشان
+
+287
+00:21:18,380 --> 00:21:21,640
+يغمرها عشان يعمل لها incubation. ما بنعمل أنا ...
+
+288
+00:21:21,640 --> 00:21:25,860
+يعني الماء اللي ... اللي في الـ incubator أو في الـ
+
+289
+00:21:25,860 --> 00:21:29,380
+water bath ... الماء اللي في الـ water bath ما بنفع
+
+290
+00:21:29,380 --> 00:21:33,380
+أنه يكون يدوب ملامس الـ tube أو مش ملامسها، هي أنا
+
+291
+00:21:33,380 --> 00:21:37,610
+ما بكون عم بعمل incubation، تمام؟ Raise the
+
+292
+00:21:37,610 --> 00:21:42,750
+temperature to تسعين درجة or higher at once a week
+
+293
+00:21:42,750 --> 00:21:45,690
+for half an hour for the purpose of
+
+294
+00:21:45,690 --> 00:21:49,630
+decontamination. بحكيلك مثلا مرة في الأسبوع لمدة
+
+295
+00:21:49,630 --> 00:21:54,230
+مثلا نص ساعة، ارفعي درجة الحرارة لتسعين و نزلّيها
+
+296
+00:21:54,230 --> 00:21:57,610
+تاني، أو مثلا شغّل الجهاز لـ ... اضبطيه على تسعين،
+
+297
+00:21:57,610 --> 00:22:01,570
+يسخّن الميّه و يرجع ... و ارجع يطفي، يخليه يبرد تاني.
+
+298
+00:22:01,920 --> 00:22:05,780
+ليش؟ هذه درجة الحرارة العالية بتقتل البكتيريا، يعني
+
+299
+00:22:05,780 --> 00:22:11,240
+in addition to mercellate. بالإضافة لـ mercellate، use
+
+300
+00:22:11,240 --> 00:22:15,160
+the lid to prevent loss of heat from the bath. لازم
+
+301
+00:22:15,160 --> 00:22:20,880
+أنا خلال الـ ... الـ ... الـ ... يعني الـ incubation time
+
+302
+00:22:20,880 --> 00:22:25,200
+أكون عم بغطي جهاز الـ water bath، حتى يعني تضلّ درجة
+
+303
+00:22:25,200 --> 00:22:29,050
+الحرارة منتظمة، ما ... ما يبرد الجهاز و يضل يرجع يسخّن
+
+304
+00:22:29,050 --> 00:22:34,190
+تاني. يعني بضمن ثباتية درجة الحرارة، و أيضا حتى ما
+
+305
+00:22:34,190 --> 00:22:37,590
+يتبخّر الماء اللي موجود فيه، و to minimize
+
+306
+00:22:37,590 --> 00:22:40,550
+particles from entering the water. يعني حتى ما
+
+307
+00:22:40,550 --> 00:22:45,870
+يضايق غبار أو مواد أو شيء يـ ... يـ ... يعني يقع في
+
+308
+00:22:45,870 --> 00:22:50,550
+الماء و يلوّثه. When removing the lid after
+
+309
+00:22:50,550 --> 00:22:54,570
+incubation, take care to avoid any water entering
+
+310
+00:22:54,570 --> 00:23:01,280
+uncapped tubes. طبعا لما أنا بـ ... بغطي الجهاز، و
+
+311
+00:23:01,280 --> 00:23:06,180
+برفعّه، لازم أتأكّد أنّه الـ ... القنابيب ما بيفوت فيها
+
+312
+00:23:06,180 --> 00:23:09,060
+البخار، الماء اللي بيتصاعد و بيتكثّف على غطاية
+
+313
+00:23:09,060 --> 00:23:12,680
+الجهاز ما يفوت داخل القنابيب. إذا لازم أنا أكون عم
+
+314
+00:23:12,680 --> 00:23:16,900
+بسكر القنابيب باستخدام الكعّب الخاص فيها، أو
+
+315
+00:23:16,900 --> 00:23:21,120
+باستخدام cotton. يعني المهم أنّه أضمن أن القنابيب
+
+316
+00:23:21,120 --> 00:23:25,960
+ما يفوت لمحتواها ماء، هيك بياثر على التركيز اللي عم
+
+317
+00:23:25,960 --> 00:23:29,970
+بشتغل فيه، و أيضا إذا أنا بدي أقرأ شدة لون أو تركيز
+
+318
+00:23:29,970 --> 00:23:35,050
+أو شيء، فممكن يأثر على النتيجة. Clean the water bath
+
+319
+00:23:35,050 --> 00:23:38,690
+regularly. طبعا لازم أنا أنظّفه باستمرار. We take
+
+320
+00:23:38,690 --> 00:23:41,750
+care not to damage the heating unit. طبعا بيكون في
+
+321
+00:23:41,750 --> 00:23:50,340
+عندي زي قدر بيسخّن زي هيك، زي سخّان أو زي مادة ...
+
+322
+00:23:50,340 --> 00:23:54,720
+يعني جزء معدني هو اللي بيسخّن و بيسخّن الميّه، تمام؟
+
+323
+00:23:54,720 --> 00:23:59,640
+فهذا الجزء طبعا مش لازم أنا أحاول ... يعني أحاول
+
+324
+00:23:59,640 --> 00:24:04,780
+أتعامل معه برفق، و مش لازم أنا أكسره أو أطلّفه.
+
+325
+00:24:05,800 --> 00:24:09,100
+Unplug the bath from the wall socket when not
+
+326
+00:24:09,100 --> 00:24:13,380
+using it. طبعا مجرد هذا الشيء بيطبّق مش بس على الـ
+
+327
+00:24:13,380 --> 00:24:18,020
+water bath، على كل الأجهزة اللي عم نستخدمها، لازم
+
+328
+00:24:18,020 --> 00:24:22,560
+أنا مجرد ما أخلص، يعني أفك الكبش من الحيّط، يعني من
+
+329
+00:24:22,560 --> 00:24:27,620
+الـ ... من الـ ... الـ ... الـ plug نفسها، أفكّها، يعني مش
+
+330
+00:24:27,620 --> 00:24:33,400
+لازم أخليه موصول بالكهربا، هذا لأنه لحتة أقل من خطر
+
+331
+00:24:33,400 --> 00:24:37,660
+الـ ... الـ ... الكهربا اللي ممكن يكون موجود في البيئة
+
+332
+00:24:37,660 --> 00:24:41,540
+اللي أنا عم بشتغل فيها. Every three to six months, I
+
+333
+00:24:41,540 --> 00:24:44,640
+check the bath for correction. طبعا يعني كل ثلاثة لستّة
+
+334
+00:24:44,640 --> 00:24:48,480
+شهور، بأتأكد من صحة درجة الحرارة اللي بيعطيني إياها هذا
+
+335
+00:24:48,480 --> 00:24:54,720
+الجهاز. زي أني مثلا أحطّ درجة حرارة بشكل random، مثلا
+
+336
+00:24:54,720 --> 00:24:59,220
+أربعين درجة، أخليه يسخّن و يصل لدرجة الأربعين و يثبت
+
+337
+00:24:59,220 --> 00:25:03,200
+عليها، بجيب thermometer و بخليه يقيس، يشوف لإنّ ...
+
+338
+00:25:03,200 --> 00:25:06,100
+أتأكّد أنّه والله الـ thermometer بيعطيني أربعين زي
+
+339
+00:25:06,100 --> 00:25:10,140
+ما الجهاز بيعطيني أربعين ولا لأ، فهذا يعني بتأكّد من
+
+340
+00:25:10,140 --> 00:25:16,760
+صحة درجات الحرارة اللي بيصل لها الجهاز. If you are
+
+341
+00:25:16,760 --> 00:25:21,020
+using a boiling water bath and oven, be sure you
+
+342
+00:25:21,020 --> 00:25:25,420
+use heat-resistant glass or plasticware. طبعا إذا
+
+343
+00:25:25,420 --> 00:25:28,800
+أنا كنت مستخدم، سواء كان oven أو حتى water bath
+
+344
+00:25:28,800 --> 00:25:34,160
+و يوصل لدرجة حرارة الغليان، لازم الـ glass اللي أنا
+
+345
+00:25:34,160 --> 00:25:38,560
+بيه، اللي بستخدمها، أو حتى سواء كانت plastic، لازم
+
+346
+00:25:38,560 --> 00:25:42,020
+يكون plastic مقوّى، بيتحمّل درجات حرارة عالية، و أيضا
+
+347
+00:25:42,020 --> 00:25:45,680
+نفس الشيء لازم يكون heat-resistant glass. يعني مثلا
+
+348
+00:25:45,680 --> 00:25:49,800
+يكون الـ ... الأنابيب الزجاجية مصنوعة من الزجاج الـ
+
+349
+00:25:49,800 --> 00:25:54,280
+pyrex، اللي هي أصلا الـ ... فعليا هي هذه المادة اللي
+
+350
+00:25:54,280 --> 00:25:58,140
+احنا بنستخدمها في الأنابيب الزجاجية اللي بتشتغلوا
+
+351
+00:25:58,140 --> 00:26:03,020
+فيها، و في الـ flasks و في الـ ... السلندرات و كل شيء
+
+352
+00:26:03,020 --> 00:26:06,020
+زجاجي عندنا من الـ pyrex، يعني هو heat-resistant.
+
+353
+00:26:11,570 --> 00:26:18,730
+طبعا أثناء إدخال و خروج أي أدوات و أنابيب من الـ
+
+354
+00:26:18,730 --> 00:26:21,110
+incubator، إذا أنا عم بشتغل على درجة حرارة عالية،
+
+355
+00:26:21,110 --> 00:26:25,590
+لازم أكون مستخدمة كفّات خاصة، لحتى ما أؤذي نفسي أو
+
+356
+00:26:25,590 --> 00:26:31,620
+أحرق نفسي. و incubator اللي هو الحاضنة أو الجهاز
+
+357
+00:26:31,620 --> 00:26:35,840
+الأعلى، الحاضن، أو الحاضن، الـ incubator، طبعا بيعطيني
+
+358
+00:26:35,840 --> 00:26:40,600
+incubation، و طبعا على controlled temperature، على درجة
+
+359
+00:26:40,600 --> 00:26:45,320
+حرارة مضبوطة. ولكن مش لتفاعل، هو لأجل
+
+360
+00:26:45,320 --> 00:26:49,200
+bacteriological culture، لشغل مزرعة البكتيريا. لما
+
+361
+00:26:49,200 --> 00:26:55,320
+أنا بدي أعزل بكتيريا أو فطر أو يعني ميكروب من عينة
+
+362
+00:26:55,320 --> 00:27:03,140
+مريض. طبعا لازم أنا أحطّ العينة، أضيف العينة تبعته
+
+363
+00:27:03,140 --> 00:27:08,040
+على مادة مغذية، تنعش و تثري نمو الميكروب.
+
+364
+00:27:11,590 --> 00:27:17,270
+أيضا يعني ... يعني أعمل لها incubation لحتى تنمو
+
+365
+00:27:17,270 --> 00:27:22,610
+على درجة حرارة مثلا 37، لـ blood transfusion، لحفظ
+
+366
+00:27:22,610 --> 00:27:27,790
+وحدات الدم. لبعض التفاعلات اللي في قسم السيرولوجي،
+
+367
+00:27:27,790 --> 00:27:31,230
+اللي هو الأمصال. لقسم الـ hematology، اللي هو علم
+
+368
+00:27:31,230 --> 00:27:35,310
+الدم. لقسم الـ chemistry. يعني أحيانا مثلا بدي
+
+369
+00:27:35,310 --> 00:27:40,230
+incubation سريع على درجة حرارة 37، بدل ما أستنى لسّة
+
+370
+00:27:40,230 --> 00:27:45,110
+بدي أسخّن water bath و أصل لدرجة حرارة 37، الـ
+
+371
+00:27:45,110 --> 00:27:47,770
+incubator إذا موجود عندكم already، غالبا بيكون
+
+372
+00:27:47,770 --> 00:27:50,810
+مضبوط على درجة حرارة 37، اللي هي درجة حرارة نموّ
+
+373
+00:27:50,810 --> 00:27:54,330
+البكتيريا و الفطريات و الميكروبات، و في الـ ... في الـ
+
+374
+00:27:54,330 --> 00:27:58,270
+cultures. فبالتالي يعني عادي، سريع جدا، ممكن أفوّت
+
+375
+00:27:58,270 --> 00:28:00,790
+الأنابيب تبعيتي، أعمل لها incubation، الوقت اللي بدّي إياه،
+
+376
+00:28:00,790 --> 00:28:04,500
+و أطلعها. في حالة أن أنا لو بشتغل فحوصات chemistry،
+
+377
+00:28:04,500 --> 00:28:10,540
+كيميائية، for bacteriological culture, an incubator is
+
+378
+00:28:10,540 --> 00:28:14,980
+required, whereas for other tests a dry heat block
+
+379
+00:28:14,980 --> 00:28:22,220
+or water bath may be used. حكينا أنّه الـ ... في الـ ...
+
+380
+00:28:22,220 --> 00:28:24,920
+في الـ bacteriological culture، في المزارع البكتيرية،
+
+381
+00:28:24,920 --> 00:28:30,130
+احنا بنستخدم الـ incubator. لكن في الفحوصات الأخرى، ممكن
+
+382
+00:28:30,130 --> 00:28:35,590
+أستخدم اللي هو الـ dry heat block أو الـ dry bath
+
+383
+00:28:35,590 --> 00:28:39,310
+أو الـ incubator العادي.
+
+384
+00:28:41,940 --> 00:28:46,100
+و غالبا بيكون usually على 37 درجة مضبوط، و حكينا
+
+385
+00:28:46,100 --> 00:28:49,400
+أنّه ممكن أنا أستخدمه لـ other tests، لو أنا بدي درجة
+
+386
+00:28:49,400 --> 00:28:54,360
+حرارة 37 و بشكل سريع، يعني ممكن أستخدمه. The
+
+387
+00:28:54,360 --> 00:28:57,500
+appropriate temperature is obtained by means of
+
+388
+00:28:57,500 --> 00:29:01,940
+a temperature regulator. طبعا بصل لدرجة الحرارة اللي
+
+389
+00:29:01,940 --> 00:29:09,660
+بدّي إياها من خلال الـ ... regulator، منظم لدرجة الحرارة.
+
+390
+00:29:09,660 --> 00:29:12,720
+بتظهر عندي درجة الحرارة هنا، أنا لازم أضل أعملها
+
+391
+00:29:12,720 --> 00:29:16,560
+check باستمرار. طبعا في عندي thermostat بنظّم لي درجة
+
+392
+00:29:16,560 --> 00:29:20,36
+
+445
+00:33:20,060 --> 00:33:23,700
+أو أُوصلِه لِمِيةِ اللي هو الـ working solution الـ working
+
+446
+00:33:23,700 --> 00:33:29,200
+standard solution لما بدي استخدمه لازم أخففه بـ ..
+
+447
+00:33:29,200 --> 00:33:33,010
+بـ distilled water طبعًا، الـ buffers أيضًا تحضيرها
+
+448
+00:33:33,010 --> 00:33:39,910
+حكينا الـ controls اللي هي العينات الضابطة، الصبغات
+
+449
+00:33:39,910 --> 00:33:43,990
+المحاليل، الـ reagents في سواء كان في قسم الـ
+
+450
+00:33:43,990 --> 00:33:48,230
+chemistry، في المناعة، في الـ hematology، في الـ
+
+451
+00:33:48,230 --> 00:33:53,010
+microbiology اللي هو علم الميكروبات، والجرافيم في
+
+452
+00:33:53,010 --> 00:33:57,650
+كل الأقسام أنا بستخدم distilled water كـ .. كـ ..
+
+453
+00:33:57,650 --> 00:34:02,490
+يعني مذيب أو solution لتحضير أو كأساس يعني
+
+454
+00:34:02,940 --> 00:34:06,860
+لتحضير الـ reagents طبعًا، و أيضًا كمان الـ culture
+
+455
+00:34:06,860 --> 00:34:11,700
+media هي المادة المغذية اللي بتنمي البكتيريا، و
+
+456
+00:34:11,700 --> 00:34:16,840
+الفطريات، و لزراعة الخلايا أيضًا بتكون برضه كمان
+
+457
+00:34:16,840 --> 00:34:20,140
+عبارة عن مواد مغذية in distilled water، يعني لما
+
+458
+00:34:20,140 --> 00:34:23,960
+بدي أحضر media للبكتيريا ولا للفطريات، بوزن الـ ..
+
+459
+00:34:23,960 --> 00:34:27,340
+البودر و بأذوبه في distilled water، بأفوته يتعقم، و
+
+460
+00:34:27,340 --> 00:34:33,860
+بعدين بأفضيه في أطباق علشان أزرع عليها العينات، تمام؟
+
+461
+00:34:33,860 --> 00:34:40,160
+فالـ .. الـ reagent اللي بتستخدم في الـ blood
+
+462
+00:34:40,160 --> 00:34:44,400
+transfusion work، فشغل اللي هو نقل الدم برضه كمان
+
+463
+00:34:44,400 --> 00:34:49,320
+محتاجة distilled water، فبالتالي إذا الـ water يعني
+
+464
+00:34:49,320 --> 00:34:56,340
+بيستخدم في كتير مجالات، في حال .. أحيانًا بالأجهزة، لو
+
+465
+00:34:56,340 --> 00:35:01,660
+بدي أنظف تيوب، بده .. أحيانًا في بعض الأجهزة، خصوصًا
+
+466
+00:35:01,660 --> 00:35:05,420
+الأجهزة اللي بتسحب العينة سحب، لازم الـ .. ده اللي
+
+467
+00:35:05,420 --> 00:35:10,060
+بتسحب اللي فيها لازم نسيلها rinsing، أشرنزنج يعني
+
+468
+00:35:10,060 --> 00:35:13,560
+غمر بغسول أو بمذيب، يعني إن احنا ننظفها ما بين كل
+
+469
+00:35:13,560 --> 00:35:19,320
+عينة والتانية، أيضًا نحتاج الـ .. الـ water، شايفين كم
+
+470
+00:35:19,320 --> 00:35:24,040
+هي مهمة الـ water؟ فلازم أيضًا الأجهزة اللي تـ .. الـ
+
+471
+00:35:24,040 --> 00:35:30,720
+.. التحضير والفصل والتعقيم تكون مهمة، في عندي
+
+472
+00:35:30,720 --> 00:35:34,220
+جهاز اللي هو الـ water distilling apparatus اللي هو
+
+473
+00:35:34,220 --> 00:35:41,300
+يعني فكرة الـ .. الـ .. التنقية والفلترة
+
+474
+00:35:41,300 --> 00:35:47,570
+للماء، طبعًا هي فكرة بسيطة بتعتمد على تسخين الماء، و
+
+475
+00:35:47,570 --> 00:35:52,170
+.. فبالتالي الـ .. الـ .. الـ .. يعني هو الماء غير
+
+476
+00:35:52,170 --> 00:35:57,650
+نقي بيتعرض لحرارة، بيسخن، البخار بيتكاثف وبيتم
+
+477
+00:35:57,650 --> 00:36:03,250
+يعني نقله من خـ .. لما بيتكاثف، بيتم نقله إلى وعاء
+
+478
+00:36:03,250 --> 00:36:12,470
+نظيف، فالـ .. الـ .. هذا الماء المتكاثف طبعًا هو اللي
+
+479
+00:36:12,470 --> 00:36:14,810
+.. اللي بنعتبره distilled water لإنه خالي من
+
+480
+00:36:14,810 --> 00:36:19,190
+الشوائب والأملاح، تمام؟ لإنه لما تبخر ما تبخرت
+
+481
+00:36:19,190 --> 00:36:22,970
+معاه الأملاح والشوائب اللي موجودة فيه، ولما تكاثف
+
+482
+00:36:22,970 --> 00:36:28,270
+نتيجة يعني وجوده في .. على سطح .. على أسطح باردة
+
+483
+00:36:28,270 --> 00:36:33,230
+تمام؟ يعني ما كان موجود فيه هاي الشوائب، لذلك هيك
+
+484
+00:36:33,230 --> 00:36:37,650
+بنكون احنا حصلنا على distilled water، تمام؟ إذن
+
+485
+00:36:37,650 --> 00:36:42,230
+water distilling apparatus is an instrument that
+
+486
+00:36:42,230 --> 00:36:46,890
+used to purify impure water، هو عبارة عن جهاز
+
+487
+00:36:46,890 --> 00:36:52,910
+لتنقية الـ .. to purify impure water اللي
+
+488
+00:36:52,910 --> 00:36:56,370
+هو الماء الغير نقي، by process known as
+
+489
+00:36:56,370 --> 00:36:59,770
+distillation، من خلال عملية اسمها distillation اللي
+
+490
+00:36:59,770 --> 00:37:03,980
+احنا شرحنا مبدأها حاليًا، distillation هو عبارة عن
+
+491
+00:37:03,980 --> 00:37:07,420
+process by which impure water is boiled، بنغلي
+
+492
+00:37:07,420 --> 00:37:12,320
+المية اللي هي الـ impure، اللي هي الغير نقية، تمام؟
+
+493
+00:37:12,320 --> 00:37:17,120
+وبخارها بيتم نقله إلى أنابيب أخرى، تمام؟ هذه
+
+494
+00:37:17,120 --> 00:37:19,980
+الأنابيب مكثفة، ليش؟ لأنها بتكون طبعًا الـ ..
+
+495
+00:37:23,380 --> 00:37:30,340
+البخار هذا بيكون موجود في أنابيب باردة نوعًا ما
+
+496
+00:37:30,340 --> 00:37:35,600
+فبيتكاثف وبيتم نقله كـ pure water، تمام؟ even the
+
+497
+00:37:35,600 --> 00:37:38,900
+steam produced condensed in a cold surface، بيتم
+
+498
+00:37:38,900 --> 00:37:43,220
+تكاثفه على أسطح باردة اللي هي الـ condenser، وطبعًا
+
+499
+00:37:43,220 --> 00:37:47,120
+بيعطيني chemically pure distilled water، يعني ماء
+
+500
+00:37:47,120 --> 00:37:52,840
+خالي من الشوائب الكيميائية طبعًا، that is water from
+
+501
+00:37:52,840 --> 00:37:56,920
+which non-volatile organic and inorganic materials
+
+502
+00:37:56,920 --> 00:38:01,320
+are removed، طبعًا المواد الغير متطايرة اللي موجودة
+
+503
+00:38:01,320 --> 00:38:04,380
+سواء كانت عضوية أو غير عضوية، هيك بنكون اتخلصنا
+
+504
+00:38:04,380 --> 00:38:11,150
+منها، تمام؟ من مساوئ هذه الطريقة إنها ما بتخلصني من
+
+505
+00:38:11,150 --> 00:38:15,150
+الـ ionic gases اللي موجودة في الماء، زي إذا كان
+
+506
+00:38:15,150 --> 00:38:18,010
+موجود في ammonia أو carbon dioxide أو chlorine
+
+507
+00:38:18,010 --> 00:38:25,810
+طبعًا ما بتخلصني منها، وأيضًا أحيانًا ممكن تكون الماء
+
+508
+00:38:25,810 --> 00:38:28,050
+اللي هي الـ distilled water اللي أنا حصلت عليها
+
+509
+00:38:28,050 --> 00:38:30,650
+contaminated with non-volatile impurities، يعني
+
+510
+00:38:30,650 --> 00:38:36,430
+يكون فيها بعض الشوائب الغير متطايرة زي الـ sodium، و
+
+511
+00:38:36,430 --> 00:38:38,850
+الـ potassium، والـ calcium، والـ carbonate ion، والـ
+
+512
+00:38:38,850 --> 00:38:43,930
+sulphate ion، طبعًا، فلذلك ممكن أنا أستخدم، إذا بدي
+
+513
+00:38:43,930 --> 00:38:47,910
+يعني ماء درجة نقاوته أعلى بكثير، ممكن أستخدم أجهزة
+
+514
+00:38:47,910 --> 00:38:51,410
+ثانية غير الـ distillator، في عندي جهاز اللي هو الـ
+
+515
+00:38:51,410 --> 00:38:58,670
+gravity water filter، الـ filtration هو عبارة عن the
+
+516
+00:38:58,670 --> 00:39:02,970
+passage of liquid، مرور الماء أو السائل من خلال
+
+517
+00:39:02,970 --> 00:39:07,630
+filter، وبيتضمن مروره طبعًا لازم من خلال gravity
+
+518
+00:39:07,630 --> 00:39:13,330
+جاذبية أو حتى pressure أو ضغط، يعني، تمام؟ فبالتالي
+
+519
+00:39:13,330 --> 00:39:21,010
+بيتم filter، وبيتم .. يعني الحصول على الماء نقي نوعًا
+
+520
+00:39:21,010 --> 00:39:24,710
+ما، طيب هنا زي مثلًا هذا الجهاز الـ gravity water
+
+521
+00:39:24,710 --> 00:39:28,730
+filter، طبعًا هذا inlet، يعني من هنا بيدخل الماء
+
+522
+00:39:28,730 --> 00:39:34,530
+الغير نقي، تمام، أو المراد فلترته، وبيمر على فلاتر
+
+523
+00:39:34,530 --> 00:39:37,610
+يعني الفلاتر هذه بتختلف في أحجامها، يعني مثلًا هنا في
+
+524
+00:39:37,610 --> 00:39:42,260
+عندي صخور، يعني المسافات البينية كبيرة، بعدين بييجي
+
+525
+00:39:42,260 --> 00:39:47,000
+هنا في عندي perforated metal plate، يعني مادة
+
+526
+00:39:47,000 --> 00:39:51,620
+معدنية مثقبة، بيمر من خلالها الماء، بيمر على الـ ..
+
+527
+00:39:51,620 --> 00:39:56,620
+على رمل، تمام، واحنا بنعرف إن الرمل بيستخدم كـ filter
+
+528
+00:39:56,620 --> 00:40:00,320
+يعني الماء اللي .. اللي بيطلع من الينابيع من الرمل
+
+529
+00:40:00,320 --> 00:40:07,400
+بيكون كثير نقي، لأن الرمل ممكن يشتغل كـ filter، تمام؟
+
+530
+00:40:08,750 --> 00:40:12,770
+فهنا بالنهاية بيطلع عندي .. بيبدأ يرتفع منسوب الـ
+
+531
+00:40:12,770 --> 00:40:17,790
+.. الـ water، كل ما ضغطنا ماء أكثر، تمام؟ بيدخل
+
+532
+00:40:17,790 --> 00:40:20,970
+الماء، وبنضغط عليه إما بـ .. يعني إما بينزل بفعل
+
+533
+00:40:20,970 --> 00:40:23,930
+الجاذبية، أو أنا بـ .. بأعمل apply pressure، يعني
+
+534
+00:40:23,930 --> 00:40:27,250
+بيكون في عندي ضغط على الـ .. المقفة، بيرتفع في ..
+
+535
+00:40:27,250 --> 00:40:30,890
+بيبدأ يطلع، هي عبر من خلال الـ rock، من خلال الـ ..
+
+536
+00:40:30,890 --> 00:40:34,470
+الـ .. المعدن، من خلال الـ sand، وبيبدأ يرتفع كـ في
+
+537
+00:40:34,470 --> 00:40:41,590
+يعني يرشح على سطح الـ sand كـ ماء مفلتر أو يعني منقى
+
+538
+00:40:41,590 --> 00:40:45,340
+وبعد هيك بيخرج من خلال الـ outlet، تمام؟ الـ
+
+539
+00:40:45,340 --> 00:40:48,640
+filtrate هذا طبعًا الـ .. الـ .. الـ .. ناتج الفلترة
+
+540
+00:40:48,640 --> 00:40:52,780
+بنسميه filtrate، طبعًا؟ وهذا بدي .. المصطلح هتستخدمه
+
+541
+00:40:52,780 --> 00:40:58,580
+كثير في التحليل، يعني هنـ .. هنfilter محلول، ونـ
+
+542
+00:40:58,580 --> 00:41:02,320
+filter مية، ونfilter صبغات، ونfilter أشياء كثيرة،
+
+543
+00:41:02,320 --> 00:41:07,300
+فالناتج الفلترة بنسميه filtrate، هو عبارة عن الـ
+
+544
+00:41:07,300 --> 00:41:12,440
+liquid that has passed through the filter، إذا
+
+545
+00:41:12,440 --> 00:41:17,760
+المحلول اللي خرج أو عدى من خلال الـ filter بنسميه
+
+546
+00:41:17,760 --> 00:41:22,000
+filtrate، the purpose of filtration، طبعًا إيش وظيفة
+
+547
+00:41:22,000 --> 00:41:25,000
+الـ filtration؟ to remove particulate matter، يعني
+
+548
+00:41:25,000 --> 00:41:30,570
+لحتى يعني أتخلص من الشوائب والمواد الكبيرة نوعًا ما
+
+549
+00:41:30,570 --> 00:41:35,690
+زي الـ bacteria، والـ parasite، والـ microorganism، زي
+
+550
+00:41:35,690 --> 00:41:40,470
+لو ما كان فيه شوائب أو يعني أوساخ في داخل الماء
+
+551
+00:41:40,470 --> 00:41:45,630
+ممكن أتخلص منها بهذا الشكل، لكن ما بتخلصنيش من خلال
+
+552
+00:41:45,630 --> 00:41:49,350
+خلال هذه العملية من الـ dissolved salt، يعني الأملاح
+
+553
+00:41:49,350 --> 00:41:53,070
+اللي ذائبة، تمام؟ يعني ممكن أتخلص من بعض الأملاح من
+
+554
+00:41:53,070 --> 00:41:57,090
+خلال مثلًا إما الـ distillation .. عملية الـ
+
+555
+00:41:57,090 --> 00:42:00,990
+distillation، أو من خلال اللي هي الـ de-ionization
+
+556
+00:42:00,990 --> 00:42:05,750
+تمام؟ طيب، جدير بالذكر أنه أنا حكيت لكم مثلًا إذا كان
+
+557
+00:42:05,750 --> 00:42:11,710
+أنا محتاجة تنقية أكثر، ممكن أستخدم أجهزة بتنقي أكثر
+
+558
+00:42:11,710 --> 00:42:16,270
+وأحيانًا ممكن أنا أحصل على، يعني لحتى أضمن أنه
+
+559
+00:42:16,270 --> 00:42:22,000
+الماء مفلتر جيدًا، أعمل double distillation، وكثير
+
+560
+00:42:22,000 --> 00:42:25,460
+أحيانًا بنحتاج الـ double distilled water، يعني ماء
+
+561
+00:42:25,460 --> 00:42:29,800
+مقطر مرتين، يعني الماء المقطر اللي هنا أنا رجعت
+
+562
+00:42:29,800 --> 00:42:34,040
+غليته مرة وكثفته، تمام؟ هذا بنسميه double
+
+563
+00:42:34,040 --> 00:42:38,600
+distilled water، خصوصًا في الفحوصات اللي هي الـ .. الـ
+
+564
+00:42:38,600 --> 00:42:43,940
+molecular، في فحوصات الـ molecular اللي هي الفحوصات
+
+565
+00:42:43,940 --> 00:42:48,370
+الجينية، أنا أحيانًا بشتغل بأنزيمات، أي تركيز لأملاح
+
+566
+00:42:48,370 --> 00:42:52,330
+أو معادن في داخل المية ممكن يأثر على تنشيط هذه
+
+567
+00:42:52,330 --> 00:42:57,390
+الأنزيمات، فبالتالي بكون محتاجة double distilled
+
+568
+00:42:57,390 --> 00:43:04,350
+water، طب الـ deionizer، إيش يعني نازع للأيونات؟ دي
+
+569
+00:43:04,350 --> 00:43:10,710
+نازع، تمام؟ deionizer يعني نازع للأيونات، تمام؟
+
+570
+00:43:10,710 --> 00:43:16,390
+deionizer هو عبارة عن apparatus to produce ion
+
+571
+00:43:16,390 --> 00:43:21,510
+-free water، تمام؟ لحتى تنتج لي ماء خالي أو يعني
+
+572
+00:43:21,510 --> 00:43:27,570
+free of ions، تمام؟ إذا عملية الـ deionization
+
+573
+00:43:30,320 --> 00:43:35,720
+هنا deionization، تمام، هي عملية is a process in
+
+574
+00:43:35,720 --> 00:43:40,400
+which chemically impure water، الماء اللي غير نقي
+
+575
+00:43:40,400 --> 00:43:46,760
+فيه شوائب كيميائية، تمام، بيمر على anion and cation
+
+576
+00:43:46,760 --> 00:43:51,740
+resins، تمام، وبنهاية أنا بحصل على ion-free water
+
+577
+00:43:51,740 --> 00:43:56,760
+إيش يعني anion and cation resins؟ يعني عبارة عن ..
+
+578
+00:43:56,760 --> 00:44:02,310
+يعني زي أقطاب فيها أنيونات وفيها كاتيونات، تمام؟
+
+579
+00:44:02,310 --> 00:44:06,370
+هلأ لما .. بس نشوف الصورة هنوضح أكثر أو هتتضح
+
+580
+00:44:06,370 --> 00:44:12,010
+الفكرة أكثر، Deionized water has low electrical
+
+581
+00:44:12,010 --> 00:44:17,500
+conductivity، قليلة أو شبه معدومة، ليش؟ لأن احنا
+
+582
+00:44:17,500 --> 00:44:20,460
+نزعنا الأيونات اللي فيها، هو إيش اللي بيخلي الماء
+
+583
+00:44:20,460 --> 00:44:23,740
+موصل للكهربا غير الأيونات اللي بتكون موجودة فيه
+
+584
+00:44:23,740 --> 00:44:31,530
+near neutral pH، بيكون الـ pH متعادل، about 7، وطبعًا
+
+585
+00:44:31,530 --> 00:44:35,210
+free from water soluble salt، بيكون خالي من الأملاح
+
+586
+00:44:35,210 --> 00:44:39,310
+وخالي من الأيونات، بس ممكن ما بيكون معقم، تمام، أنا
+
+587
+00:44:39,310 --> 00:44:42,730
+بحتاج أفوته جهاز تعقيم عشان أعقمه بعد ما أنزع
+
+588
+00:44:42,730 --> 00:44:46,330
+الأيونات منه، لو أنا محتاجة sterile and deionized
+
+589
+00:44:46,330 --> 00:44:50,250
+water، لكن إذا محتاجة deionized water لحال، فخلاص
+
+590
+00:44:50,250 --> 00:44:55,510
+بمرره من خلال جهاز الـ deionizer، وبعدين بستخدمه
+
+591
+00:44:55,510 --> 00:45:02,070
+طيب، الـ cations اللي بتكون ممكن تكون موجودة في الماء
+
+592
+00:45:02,070 --> 00:45:07,890
+which may be present in water، زي ash، يعني أجزاء
+
+593
+00:45:07,890 --> 00:45:11,910
+مشحونة موجبة، cations، يعني هتكون زي الـ calcium، الـ
+
+594
+00:45:11,910 --> 00:45:16,580
+magnesium، الـ sodium، تمام؟ وهذه أيضًا بيتم ..
+
+595
+00:45:16,580 --> 00:45:20,260
+يعني بيهـ .. احنا حكينا هتمر على كاتيون وأن يون
+
+596
+00:45:20,260 --> 00:45:25,720
+resins، تمام؟ فهتمر على الكاتيون، وياخذها الكاتيون
+
+597
+00:45:25,720 --> 00:45:31,070
+تبع الجهاز، وبيعطي الماء كاتيون آخر، والـ anions اللي
+
+598
+00:45:31,070 --> 00:45:34,790
+بتكون في الماء، زي الـ sulphates، والـ bicarbonate، والـ
+
+599
+00:45:34,790 --> 00:45:38,650
+silicate، تمام، والـ nitrate، والـ chloride، أي أجزاء
+
+600
+00:45:38,650 --> 00:45:42,910
+شحنتها سالبة، بيتم استبدالها بأن يون أخرى من الجهاز
+
+601
+00:45:42,910 --> 00:45:47,730
+يعني مثلًا هي هنا الجهاز موجود فيه cation وموجود فيه
+
+602
+00:45:47,730 --> 00:45:51,550
+anion resin، الـ cation resin بيكون موجود فيه فقط
+
+603
+00:45:51,550 --> 00:45:55,170
+أيوانات الـ hydrogen، تمام، الـ protonated، يعني بيكون
+
+604
+00:45:55,170 --> 00:45:59,520
+موجود فيه، هو الـ .. الـ .. الـ anion resin تبع الجهاز
+
+605
+00:45:59,520 --> 00:46:04,500
+بيكون فيه OH، تمام؟ بيكون فيه OH، هلأ مجرد ما أنا
+
+606
+00:46:04,500 --> 00:46:07,960
+أمرر الماء، هي قطرة الماء اللي مرت مثلًا، بنعتبر
+
+607
+00:46:07,960 --> 00:46:10,940
+إنه هذه كمية الماء اللي أنا بدي أعمل لها de
+
+608
+00:46:10,940 --> 00:46:14,180
+-ionization، هلأ هي موجودة فيها أنيونات زي الـ
+
+609
+00:46:14,180 --> 00:46:17,760
+sodium، وموجودة فيها .. sorry، موجودة فيها أنيونات زي
+
+610
+00:46:17,760 --> 00:46:22,640
+الـ chloride، وكاتيونات زي الـ sodium، طبعًا، طيب، مرت على
+
+611
+00:46:22,640 --> 00:46:26,660
+الـ resin تبع الجهاز اللي هو عبارة عن إيش؟ كاتيونات
+
+612
+00:46:26,660 --> 00:46:32,280
+هيدروجين، وأن يونات هيدروكسيل، طبعًا، فإيش هيعمل
+
+613
+00:46:32,280 --> 00:46:37,760
+الجهاز؟ هيروح ماخذ الصوديوم من الماء، ومعطيها بداله
+
+614
+00:46:37,760 --> 00:46:44,220
+اللي هو H plus، طبعًا، استبدال كاتيون بكاتيون، أخد صوديوم
+
+615
+00:46:44,220 --> 
+
+667
+00:50:12,330 --> 00:50:18,690
+لختام محاضرات مساق المقدمة في التحاليل الطبية أتمنى
+
+668
+00:50:18,690 --> 00:50:24,690
+أنكم تكونوا استفدتم واستمتعتم، أتمنى ذلك، إنه في ..
+
+669
+00:50:24,690 --> 00:50:30,130
+بالمساق، وإن شاء الله يعني بتطلعوا منه بتكونوا
+
+670
+00:50:30,130 --> 00:50:35,290
+على خبرة ودراية كافية تمكنكم من أن تأخذوا
+
+671
+00:50:35,290 --> 00:50:39,870
+المساقات الأخرى التي تعتمد على هذا المساق، والتي
+
+672
+00:50:39,870 --> 00:50:43,610
+من خلاله أيضًا، يعني أنتم ستنطلقون إلى التخصصات
+
+673
+00:50:43,610 --> 00:50:47,830
+الأخرى الموجودة تحت مسمى علوم طبية مخبرية.
+
+674
+00:50:50,320 --> 00:50:57,220
+سأطالب، سأطالبكم بواجب: أن تختاروا جهازًا يُستخدم
+
+675
+00:50:57,220 --> 00:51:03,740
+في الـ medical laboratories، في المختبرات الطبية، طبعًا
+
+676
+00:51:03,740 --> 00:51:07,230
+الجهاز سواء كان incubator، oven، balance،
+
+677
+00:51:08,170 --> 00:51:13,750
+centrifuge، spectrophotometer أو حتى أي جزء آخر
+
+678
+00:51:13,750 --> 00:51:21,330
+لم نذكره، مثلًا، ممكن أن تختاروه وتـ
+
+679
+00:51:21,330 --> 00:51:29,250
+تبحثوا عنه، تكتبوا لي عن فكرة عمله واستخدامه، و
+
+680
+00:51:29,250 --> 00:51:36,850
+مبدأ عمله بشكل مبسط، تمام؟ يعني أريد واجبًا هكذا من
+
+681
+00:51:36,850 --> 00:51:41,230
+صفحة إلى صفحة ونصف عن جهاز يُستخدم في الـ medical
+
+682
+00:51:41,230 --> 00:51:44,330
+laboratories، سواء من التي ذكرناها في المساق أو
+
+683
+00:51:44,330 --> 00:51:49,290
+سواء إذا واجهتُم أثناء بحثكم جهازًا لم نتطرق إليه،
+
+684
+00:51:49,290 --> 00:51:53,350
+ممكن أن يُغطى أيضًا من خلال هذا التقرير أو
+
+685
+00:51:53,350 --> 00:51:59,770
+هذا الواجب المنزلي، أتمنى لكم التوفيق، يعطيكم ألف عافية، و
+
+686
+00:51:59,770 --> 00:52:00,910
+هنيئًا لكم، إن شاء الله موفقون.
diff --git a/PL9fwy3NUQKwY9jl8xw-bWkVZkWcGGyspd/RK30qovrvdg_postprocess.srt b/PL9fwy3NUQKwY9jl8xw-bWkVZkWcGGyspd/RK30qovrvdg_postprocess.srt
new file mode 100644
index 0000000000000000000000000000000000000000..de9e68c4e730b7322dae61590d49d2b9784cee36
--- /dev/null
+++ b/PL9fwy3NUQKwY9jl8xw-bWkVZkWcGGyspd/RK30qovrvdg_postprocess.srt
@@ -0,0 +1,2744 @@
+1
+00:00:06,980 --> 00:00:10,700
+السلام عليكم ورحمة الله وبركاته طالبات العزيزات
+
+2
+00:00:10,700 --> 00:00:14,680
+اليوم ان شاء الله رح نتحدث عن ال essential lab
+
+3
+00:00:14,680 --> 00:00:20,360
+instruments تمام؟ رح نتطرق لبعض أجهزة المختبر و
+
+4
+00:00:20,360 --> 00:00:27,450
+نتعرف عن مبدأ عملها، طريقة عملها و استخدامهابما
+
+5
+00:00:27,450 --> 00:00:34,470
+انه كان المساق كله تعريفي بالمختبر و أدواته و
+
+6
+00:00:34,470 --> 00:00:40,190
+أجهزته و يعني كان في بعض الفحوصات الأساسية اللي
+
+7
+00:00:40,190 --> 00:00:44,650
+احنا اتعلمناها و كنا بنستخدم بعض الأجهزة و اتعرفنا
+
+8
+00:00:44,650 --> 00:00:49,390
+على centrifuge و مبدأ عمل و استخدام و اتعرفنا على
+
+9
+00:00:49,390 --> 00:00:54,570
+spectrophotometer و ال microscope كمان اليوم راح
+
+10
+00:00:54,570 --> 00:00:58,600
+نذكر بعض الأجهزة الأخرىالسلام عليكمزي ال balances
+
+11
+00:00:58,600 --> 00:01:04,880
+طبعا الموازين ال balances هي عبارة عن essential
+
+12
+00:01:04,880 --> 00:01:11,680
+laboratory instruments أجهزة أساسية في المختبر و
+
+13
+00:01:11,680 --> 00:01:15,760
+طبعا هي widely used for weighing of various
+
+14
+00:01:15,760 --> 00:01:20,400
+substances طبعا احنا بنستخدمها لوزن المواد سواء
+
+15
+00:01:20,400 --> 00:01:24,660
+كانت هذه المواد powder, crystals المهم مواضة صلبة
+
+16
+00:01:24,660 --> 00:01:29,720
+يتم وزنها باستخدام ال balancesتمام و طبعا لحتى
+
+17
+00:01:29,720 --> 00:01:35,320
+نحضر reagents و سبغات culture media اللي هي المادة
+
+18
+00:01:35,320 --> 00:01:39,040
+المغذية لتنمية البكتيريا و التعرف عليها و تشخيص
+
+19
+00:01:39,040 --> 00:01:44,500
+الأمراض البكتيرية و حتى الفطريات بيلزمنا اني لحتى
+
+20
+00:01:44,500 --> 00:01:48,600
+احضر وسط ينمو عليه البكتيريا او الفطر ان انا اوزن
+
+21
+00:01:48,600 --> 00:01:54,940
+من هاي المادة المغذية و ادوبها ب ..this solvent
+
+22
+00:01:54,940 --> 00:02:00,280
+تمام اذا ال balances بتستخدم for weighing
+
+23
+00:02:00,280 --> 00:02:05,020
+accurately and precisely طبعا within a needed
+
+24
+00:02:05,020 --> 00:02:10,800
+range طبعا بيكون فيها الميزان range بيتحمل وزن من
+
+25
+00:02:10,800 --> 00:02:14,800
+الى وطبعا انا بستخدمه طبقا لهذا ال range نفس فكرة
+
+26
+00:02:14,800 --> 00:02:17,420
+ال by bit يعني انا بستخدم ال by bit علشان انقل
+
+27
+00:02:17,420 --> 00:02:22,850
+volume وكل by bit بيكون لها range خاص فيهاتمام إذا
+
+28
+00:02:22,850 --> 00:02:29,590
+ال balances هي عبارة عن موازين أو ميزان يستخدم في
+
+29
+00:02:29,590 --> 00:02:33,350
+وزن المواد الصلبة powder أو crystal سواء لو كنت
+
+30
+00:02:33,350 --> 00:02:40,730
+بدي أحضر محليل reagents صبغات buffer محليل ملحية
+
+31
+00:02:40,730 --> 00:02:45,090
+سواء كان بدي أحضر cultural media وطبعا في عندي
+
+32
+00:02:45,090 --> 00:02:49,010
+نوعين من هاي الموازينال rough balances أو ال
+
+33
+00:02:49,010 --> 00:02:52,870
+mechanical balances و ال analytical balances تمام
+
+34
+00:02:52,870 --> 00:02:57,050
+نيجي نتعرف على ال rough balances هي عبارة عن طبعا
+
+35
+00:02:57,050 --> 00:03:03,130
+mechanical يعني يتم الوزن و الضبط يدوي و يحتوي على
+
+36
+00:03:03,130 --> 00:03:06,910
+ال .. هذا الميزان على sliding scaleهنا بيكون في
+
+37
+00:03:06,910 --> 00:03:10,630
+تدريج وبيكون في عندي قطع بتنزلق على هذا التدريج
+
+38
+00:03:10,630 --> 00:03:15,690
+بتتحرك يمينا ويسارا تمام؟ مجرد ما انا اوضع المعدة
+
+39
+00:03:15,690 --> 00:03:20,930
+المراد وزنها ببدأ احرك هذه القطع اللي بتنزلق على
+
+40
+00:03:20,930 --> 00:03:26,990
+ال scale على التدريج لحد ما المؤشر هنا يصل لمنتصف
+
+41
+00:03:26,990 --> 00:03:31,390
+يعني انه خلاص صار في balanceتمام صار في اتزان في
+
+42
+00:03:31,390 --> 00:03:36,890
+ال .. هذا الميزان و بقى اخد .. بعرف اللي يعني لحد
+
+43
+00:03:36,890 --> 00:03:39,970
+ما وين وصلت القطعة اللي انزلقت بقى باخد القراءة
+
+44
+00:03:39,970 --> 00:03:43,410
+على التدريج و بعرف انه هذا الوزن طبعا مثل ما انتوا
+
+45
+00:03:43,410 --> 00:03:47,270
+شايفين هذا ال rough balancesمش كتير sensitive يعني
+
+46
+00:03:47,270 --> 00:03:50,730
+مابيقدر يفرقلي ما بين ال .. ال .. يعني الفروق
+
+47
+00:03:50,730 --> 00:03:56,710
+البسيطة في ال .. في الأوزان و أيضا يعني بيستخدم ل
+
+48
+00:03:56,710 --> 00:04:00,030
+ال large amount يعني انا لو بدي أوزن كميات أو
+
+49
+00:04:00,030 --> 00:04:04,190
+أوزان عالي أو أوزان كبيرة و لما بدي يكون عندي هاي
+
+50
+00:04:04,190 --> 00:04:10,420
+الأوزان مش مطلوبيعني high degree of accuracy مش ..
+
+51
+00:04:10,420 --> 00:04:16,860
+مش متطلب مني أنه يكون عندى يعني درجة دقة و صحة
+
+52
+00:04:16,860 --> 00:04:20,960
+عالية فممكن أنا أستخدم هذه ال rough balances طبعا
+
+53
+00:04:20,960 --> 00:04:24,560
+هاي ال rough balances ما بتحتاج main electricity
+
+54
+00:04:24,560 --> 00:04:27,160
+ما بتحتاج أن أنا أشبكها بالكهرب أو أن يكون في
+
+55
+00:04:27,160 --> 00:04:34,320
+عندها battery .. بطاريةطبعا حكينا إنها هي less
+
+56
+00:04:34,320 --> 00:04:40,420
+expensive مش غالية وكمان مش sensitive طبعا إلا إنه
+
+57
+00:04:40,420 --> 00:04:45,700
+في بعض الأنواع ممكن يعني يفرق ما بين ال .. ال ..
+
+58
+00:04:45,700 --> 00:04:50,560
+يعني الموازين وممكن يوزن لي لحد point one gram of
+
+59
+00:04:50,560 --> 00:04:55,900
+substanceتمام؟ هذا ال .. ما يتعلق بال mechanical
+
+60
+00:04:55,900 --> 00:04:59,000
+balance ال analytical balance لأ هو دقيق جدا هو
+
+61
+00:04:59,000 --> 00:05:03,420
+اللي اشتغلنا عليه لما كنا نوزن الماء و نعمل
+
+62
+00:05:03,420 --> 00:05:07,960
+calibration لل pipet طبعا هو دقيق جدا و طبعا
+
+63
+00:05:07,960 --> 00:05:13,400
+بيحتاج مما ان هو analytical و electronic لازم انا
+
+64
+00:05:13,400 --> 00:05:18,610
+يكون مشبوك بالكهرباو ممكن يكون او بيشتغل على
+
+65
+00:05:18,610 --> 00:05:22,350
+بطارية need main electricity او battery و برضه
+
+66
+00:05:22,350 --> 00:05:26,270
+كمان ان هو highly sensitive حساس جدا ممكن يفرقلي
+
+67
+00:05:26,270 --> 00:05:31,190
+بين الأوزان بدقة يعني لو point واحد فارق بالوزن
+
+68
+00:05:31,190 --> 00:05:37,010
+ممكن يوضحلي ياه و طبعا high accurate and precise
+
+69
+00:05:37,010 --> 00:05:42,770
+يعني instrument و طبعا بيوزن بمبدأ ال electro ..
+
+70
+00:05:42,770 --> 00:05:46,980
+ال electromagnetic forceطمام؟ يعني أنا مش محتاجة
+
+71
+00:05:46,980 --> 00:05:50,560
+أن أنا أعاير إشي بمقابل الوزن علشان أ .. يعني ما
+
+72
+00:05:50,560 --> 00:05:53,780
+.. مش .. مش محتاجة أحصل على المكيل هاي معدنية و
+
+73
+00:05:53,780 --> 00:05:57,840
+أحطهم و أكيل أو أن أنا أحرك على sliding scale لبن
+
+74
+00:05:57,840 --> 00:06:01,760
+ما يتزن المؤشر إلى نص الميزان زي ما بنعمل بال ..
+
+75
+00:06:01,760 --> 00:06:07,630
+ال .. ال ..ال .. ال rough balances طبعا مش محتاجة
+
+76
+00:06:07,630 --> 00:06:13,310
+لهذا الاشي طبعا ال rough balances ممكن يكون ب one
+
+77
+00:06:13,310 --> 00:06:19,850
+ban يعني إله دفة واحدة أو double ban طبعا دفتانطيب
+
+78
+00:06:19,850 --> 00:06:24,190
+فبالتالي إحنا مش محتاجين إننا يعني إشي أعاير فيه
+
+79
+00:06:24,190 --> 00:06:28,030
+sliding scale أو أوزان معيارية إنما بطريقة ..
+
+80
+00:06:28,030 --> 00:06:33,010
+بمبدأ عملي الـ electromagnetic force ممكن يعني ..
+
+81
+00:06:33,010 --> 00:06:37,510
+يعني كام بي .. يعني ال .. المادة ينشأ عنها
+
+82
+00:06:37,510 --> 00:06:42,650
+magnetic forceبيعطيني وزن لأ لها، تمام؟ طيب
+
+83
+00:06:42,650 --> 00:06:46,710
+بيستخدم لما أنا بدي أوزن أوزان صغيرة جدا و لما
+
+84
+00:06:46,710 --> 00:06:53,110
+بيكون في عندي ال high accuracy يعني مطلوبة، تمام؟
+
+85
+00:06:53,110 --> 00:06:56,910
+when great accuracy is requiredعلى عكس اللي هي ال
+
+86
+00:06:56,910 --> 00:07:01,550
+rough balances طيب أنا كيف بدي استخدم هذا الميزان
+
+87
+00:07:01,550 --> 00:07:06,050
+و كيف انا بدي اعمله care اهتم فيه؟ طبعا ال balance
+
+88
+00:07:06,050 --> 00:07:10,190
+هو عبارة عن جهاز instrument حساس delicate
+
+89
+00:07:10,190 --> 00:07:14,630
+instrumentوبيحتاج أنه أنا يعني أتبع التعليمات
+
+90
+00:07:14,630 --> 00:07:18,810
+أثناء الاستخدام أستخدمه بشكل صحيح correct use و
+
+91
+00:07:18,810 --> 00:07:24,890
+أعتني فيه فطبعا أول حاجة لازم قبل البدء بتشغيل أي
+
+92
+00:07:24,890 --> 00:07:31,170
+جهاز أو باستخدام أي جهاز هذا يعني ينطبق على الكلمن
+
+93
+00:07:31,170 --> 00:07:34,810
+ضمنهم ال balances أنه أنا أقرأ ال manufacturer
+
+94
+00:07:34,810 --> 00:07:39,310
+instruction طبعا بيجي catalog مع كل جهاز من الشركة
+
+95
+00:07:39,310 --> 00:07:43,830
+المصنعة و في تعليمات كيفية الاستخدام و الدبط و
+
+96
+00:07:43,830 --> 00:07:50,410
+المعايرة و كيفية التشغيل و لو كام .. حتى يعني في
+
+97
+00:07:50,410 --> 00:07:53,600
+خلل معين ممكن كيف أتفاداهكل هذه الشغلات بيكون
+
+98
+00:07:53,600 --> 00:07:57,160
+موجودة فيه ال catalog فانا لازم اقرأ هذا ال
+
+99
+00:07:57,160 --> 00:07:59,820
+catalog او ال instruction حتى كمان التركيب طريقة
+
+100
+00:07:59,820 --> 00:08:02,960
+التركيب اذا كان اجا الجهاز عبارة عن قطع و انا بدي
+
+101
+00:08:02,960 --> 00:08:06,100
+اركبهم برضه كمان بيكون موجود في ال manufacturer
+
+102
+00:08:06,100 --> 00:08:10,350
+instruction and catalogalways handle a balance
+
+103
+00:08:10,350 --> 00:08:13,010
+with a career طبعا لازم انا يعني اتعامل مع ال
+
+104
+00:08:13,010 --> 00:08:19,870
+balance و استخدمه باهتمام برعاية جدا و طبعا
+
+105
+00:08:19,870 --> 00:08:23,250
+position the balance on a firm bench away from
+
+106
+00:08:23,250 --> 00:08:28,310
+vibration طبعا انا بحاول ان انا احط الميزان على
+
+107
+00:08:28,310 --> 00:08:33,090
+بنش ثابت بعيد عن الاهتزازات يعني مايكون تحت مروحة
+
+108
+00:08:33,090 --> 00:08:36,370
+او مقابل يعني
+
+109
+00:08:37,830 --> 00:08:42,250
+الـ تكييف مثلا إذا إجا يعني طيار الهواء بيخبط فيه
+
+110
+00:08:42,250 --> 00:08:45,810
+هذا هيأثر و يكون بعيد مثلا عن التلاجة انتوا
+
+111
+00:08:45,810 --> 00:08:51,050
+بتعرفوا مثلا تلاجة، computer، أجهزة فيها hardware
+
+112
+00:08:51,050 --> 00:08:55,070
+و software ممكن إنها أيضا ان تعملي ذبذبات و
+
+113
+00:08:55,070 --> 00:08:59,530
+إهتزازات فهذا رح يأثرأيضا ببعده عن الهواء وضوء
+
+114
+00:08:59,530 --> 00:09:04,310
+الشمس ببعده انه يتعلق لرطوبة او ي .. يصير يعني ..
+
+115
+00:09:04,310 --> 00:09:07,930
+كمان حتى ببعده عن ال tap water حتى ما يجي عليه ميه
+
+116
+00:09:07,930 --> 00:09:12,070
+و يصدي فهذه الشغلات كمان يعني تدخل من ضمن ال care
+
+117
+00:09:12,070 --> 00:09:16,190
+و انه انا كيف احافظ على الجهازbefore starting to
+
+118
+00:09:16,190 --> 00:09:20,010
+weigh zero the balance طبعا قبل استخدام هذا
+
+119
+00:09:20,010 --> 00:09:24,810
+الميزان لازم انا اصفر و اذا كنت بستخدم beam
+
+120
+00:09:24,810 --> 00:09:27,610
+balance لازم اتأكد على ال position لل beam ايش
+
+121
+00:09:27,610 --> 00:09:31,170
+يعني ال beam balance؟ شوفتوا الميزان اللي بيكون
+
+122
+00:09:31,170 --> 00:09:35,370
+على رمز المحكمة او العدالة اللي هو عبارة عن دفتين
+
+123
+00:09:35,370 --> 00:09:41,400
+معلقات بجان Zero فيهم في ..يعني زي حامل بالنص
+
+124
+00:09:41,400 --> 00:09:45,800
+بالمركز طبعا انا لازم اتأكد من انه هدولة ال .. ال
+
+125
+00:09:45,800 --> 00:09:49,660
+.. الحامل اللي بالمركز و الدفتين موضوع يعني ال
+
+126
+00:09:49,660 --> 00:09:53,740
+position تبعهم كتير منيح مضبوط علشان انا استخدمهم
+
+127
+00:09:53,740 --> 00:09:57,240
+تمام؟ هو بيكون ب .. بيكون بحاجة لأوزان معيارية
+
+128
+00:09:57,240 --> 00:10:01,220
+بهذه الحالةطبعا إذا أنا بدي أوزن chemicals على
+
+129
+00:10:01,220 --> 00:10:06,260
+درجة حرارة الغرفة لازم أحط المادة المراد وزنها ب
+
+130
+00:10:06,260 --> 00:10:12,360
+container ب .. cup أو small beaker طبعا علشان ..
+
+131
+00:10:12,360 --> 00:10:15,000
+طبعا أنتوا كنتوا لما توقعت وزنه يعني أكيد لازم
+
+132
+00:10:15,000 --> 00:10:19,180
+تستخدمي cup فاضي، لازم تستخدمي beaker أو ..كنت
+
+133
+00:10:19,180 --> 00:10:25,400
+أحيان تتوزن على ورقة في النهاية أنه أنا طبعا ما ..
+
+134
+00:10:25,400 --> 00:10:28,280
+يعني ما أحط ال .. ال chemical never put the
+
+135
+00:10:28,280 --> 00:10:31,200
+chemical directly on the balance فعلا يعني على دفة
+
+136
+00:10:31,200 --> 00:10:35,460
+ال .. الميزان ما أحط المادة الكيميائية ال .. ال ..
+
+137
+00:10:35,460 --> 00:10:40,880
+المراد وزنها مباشرة مهما كان نوع هذه المادة ممنوع
+
+138
+00:10:40,880 --> 00:10:44,500
+أنا أحطها لإنها ممكن إنها ..تطلف الجهاز when
+
+139
+00:10:44,500 --> 00:10:48,200
+adding or removing a chemical remove the container
+
+140
+00:10:48,200 --> 00:10:52,740
+to avoid spilling and chemical on the balance طبعا
+
+141
+00:10:52,740 --> 00:10:56,300
+لما انا بدي اوزن لازم احط المادة المراد وزنها في
+
+142
+00:10:56,300 --> 00:10:58,840
+هذا ال container و لما بدي ارفع برفع ال container
+
+143
+00:10:58,840 --> 00:11:03,280
+كله علشان في النهاية انا بدي امنع انسكاب اي مادة
+
+144
+00:11:03,280 --> 00:11:06,900
+كيميائية او يعني انها تنكب على الميزان ممكن انها
+
+145
+00:11:06,900 --> 00:11:10,020
+تتسرب الى الداخل تعمل انسدادات او تأثر على الجهاز
+
+146
+00:11:10,020 --> 00:11:16,110
+من جواأو حتى تلوث الجهاز أو تخربه always use
+
+147
+00:11:16,110 --> 00:11:19,590
+forceps to add or remove weight طبعا انا اذا بدي
+
+148
+00:11:19,590 --> 00:11:24,130
+انقل مادة لحتى اوزنها او يعني اشي لازم انا استخدم
+
+149
+00:11:24,130 --> 00:11:27,050
+ال forceps بالملقط او حتى استخدم ال spatula اللي
+
+150
+00:11:27,050 --> 00:11:30,530
+هي المعلقة المعدنية الصغيرة المخصصة لنقل المواد
+
+151
+00:11:30,530 --> 00:11:34,980
+المراد وزنهاطيب protect the weight from dust إن
+
+152
+00:11:34,980 --> 00:11:40,940
+أنا أحاول أحافظ على الجهاز من الغبار والرطوبة وإنه
+
+153
+00:11:40,940 --> 00:11:45,980
+ما ينمو عليه الفطريات
+
+154
+00:11:45,980 --> 00:11:51,200
+ومثلا بغطي الجهاز إذا كان إله cover طبعا و الأوزان
+
+155
+00:11:51,200 --> 00:11:54,800
+اللي بوزنها بحاول إن أنا كمانيعني اللى مابدي
+
+156
+00:11:54,800 --> 00:11:57,620
+أوزنها أستخدمها أغطيها إذا مابدي أستخدمها مباشرة
+
+157
+00:11:57,620 --> 00:12:01,540
+لحتى برضه كمان أحافظ عليها ممكن أي غبراء، رطوبة أو
+
+158
+00:12:01,540 --> 00:12:06,480
+حتى يعني تعفن فيها يأثر على وزنها وجودتها ويأثر
+
+159
+00:12:06,480 --> 00:12:08,540
+على المحليل اللى أنا بدي أحضرها باستخدام هذه
+
+160
+00:12:08,540 --> 00:12:11,920
+الأوزانuse a small brush to remove any chemical
+
+161
+00:12:11,920 --> 00:12:16,580
+فهي بيكون عندي فرشاية مخصصة ل .. لكنز البان
+
+162
+00:12:16,580 --> 00:12:21,600
+للميزان و دفة الميزان لحتى مايضل عالق عليه أي
+
+163
+00:12:21,600 --> 00:12:26,060
+أجزاء من المادة المراد وزنها فبالتالي هيك أنا بضل
+
+164
+00:12:26,060 --> 00:12:30,810
+محافظة على الجهاز ال ovenال ovens اللي هي الأفران
+
+165
+00:12:30,810 --> 00:12:36,750
+أيضا بنستخدم أفران في المختبرات طبعا في عندي hot
+
+166
+00:12:36,750 --> 00:12:42,070
+air oven اللي هو ال .. ال oven اللي بي .. بي طبعا
+
+167
+00:12:42,070 --> 00:12:45,630
+فرن بس مش بيكون في حرارة يعني في نار مولعة أو في
+
+168
+00:12:45,630 --> 00:12:49,830
+لهب أو في إشي لأ هو بي .. يعني بيبدأ .. بيشتغل
+
+169
+00:12:49,830 --> 00:12:53,310
+بمبدأ عمل تدوير الهواء الساخن يعني في هوا ساخن
+
+170
+00:12:53,310 --> 00:12:58,760
+بيطلعفي الجهاز بي .. بيتم دورانه في الجهاز فبي ..
+
+171
+00:12:58,760 --> 00:13:02,640
+بيسخن الأشياء اللي .. اللي موجودة في الجهاز طبعا
+
+172
+00:13:02,640 --> 00:13:06,760
+بيستخدم ال oven for drying لتشفيف ال chemicals إذا
+
+173
+00:13:06,760 --> 00:13:11,400
+كان أنا حاسيت إنه المادة ممتصة رطوبة أو يعني مبتلة
+
+174
+00:13:11,400 --> 00:13:17,400
+أو حتى أنا بدي يعني أركزها مادة مدعبة و بدي أركزها
+
+175
+00:13:17,400 --> 00:13:21,740
+ممكن أستخدم ال .. ال oven للتشفيف و كده أيضا برضه
+
+176
+00:13:21,740 --> 00:13:27,790
+كمان إذا كان في عنديمواد زجاجية أنا غسلتها و
+
+177
+00:13:27,790 --> 00:13:31,430
+نضفتها و بدي أجففها لإنه بدي أستخدمها يعني من شروط
+
+178
+00:13:31,430 --> 00:13:35,210
+الاستخدام إنها تكون جافة تماما إذا كنت بحضر محلول
+
+179
+00:13:35,210 --> 00:13:38,650
+و لازم تكون جافة تماما و التركيز و ال .. و ال ..
+
+180
+00:13:38,650 --> 00:13:41,250
+هادي بتاعي بيعنيلي إنه يكون دقيق فبالتالي ال
+
+181
+00:13:41,250 --> 00:13:44,610
+glassware لازم تكون جافة و مافيها بقايا ماء أيضا
+
+182
+00:13:44,610 --> 00:13:49,650
+هادي لازم تجفف بال ovenأيضا يستخدم ال oven ل ال
+
+183
+00:13:49,650 --> 00:13:52,310
+sterilization إذا مش بس ل ال drying ل ال
+
+184
+00:13:52,310 --> 00:13:56,630
+sterilization كمان ل تعقيم ال glassware و ال metal
+
+185
+00:13:56,630 --> 00:13:59,750
+instrument الأدوات المعدنية و الأدوات الزجاجية
+
+186
+00:13:59,750 --> 00:14:06,230
+طبعا هو مكون من double wall من طبعا جدرانه عبارة
+
+187
+00:14:06,230 --> 00:14:14,730
+عن طبقة مزدوجة من النحاس أو المعدنطبعا و بيسخن by
+
+188
+00:14:14,730 --> 00:14:18,970
+circulation of hot air حكينا انه من خلال تدوير
+
+189
+00:14:18,970 --> 00:14:25,290
+الهواء الساخن طبعا بيكون فيها gas burner في لهب في
+
+190
+00:14:25,290 --> 00:14:29,590
+منطقة ما في الجهاز و بتسخن الهواء و الهواء طبعا
+
+191
+00:14:29,590 --> 00:14:34,630
+بيتنضخه داخل الجهاز و تدويره طبعا في عندي
+
+192
+00:14:34,630 --> 00:14:37,370
+thermometer بيقيص على .. على .. بيكون موجود على
+
+193
+00:14:37,370 --> 00:14:43,880
+جدار او على ..باب الجهاز في ال .. في الأعلى بيكون
+
+194
+00:14:43,880 --> 00:14:47,620
+a thermometer بيقيص درجة حرارة الجهاز و thermostat
+
+195
+00:14:47,620 --> 00:14:51,800
+بيعمل regulation لدرجة الحرارة طبعا؟ اذا في عندي
+
+196
+00:14:51,800 --> 00:14:56,160
+قياس لدرجة الحرارة و ضبط لهذه الدرجة طبعا انا ب ..
+
+197
+00:14:56,160 --> 00:15:00,100
+بعمله on في عندي in, on و off و في عندي هنا scale
+
+198
+00:15:00,100 --> 00:15:02,600
+بي .. انا بختار درجة الحرارة اللي انا بدي أجفف
+
+199
+00:15:02,600 --> 00:15:04,020
+عليها أو بدي أعقم عليها
+
+200
+00:15:07,160 --> 00:15:11,200
+ال water bath ال water bath هو عبارة عن a
+
+201
+00:15:11,200 --> 00:15:15,180
+instrument where water is heated and the seat
+
+202
+00:15:15,180 --> 00:15:18,480
+temperature is maintained at constant level طبعا
+
+203
+00:15:18,480 --> 00:15:23,460
+يعني أنا هنا بستخدم باستخدام درجة حرارة ثابتة بسخن
+
+204
+00:15:23,460 --> 00:15:29,170
+الماءو في داخل هذا الجهاز و بستخدمه كوسط مائي أو
+
+205
+00:15:29,170 --> 00:15:33,450
+حمام مائي طبعا ليش؟ to incubate liquid substance
+
+206
+00:15:33,450 --> 00:15:36,530
+طبعا كان في عندنا في تفاعلات ال biochemistry
+
+207
+00:15:36,530 --> 00:15:42,450
+incubate the tube على درجة حرارة كذا، تمام؟ فكان
+
+208
+00:15:42,450 --> 00:15:46,290
+لازم انك انت بعد ما تجري التفاعلتحط A على B
+
+209
+00:15:46,290 --> 00:15:50,170
+Reagent A على .. او مادة A على مادة B علشان تطلع
+
+210
+00:15:50,170 --> 00:15:53,950
+المادة C اللي هي ال .. ال .. الناتج كان لازم انك
+
+211
+00:15:53,950 --> 00:15:57,270
+انت تعملي incubation على درجة حرارة معينة طبعا
+
+212
+00:15:57,270 --> 00:16:01,370
+باستخدام ال water بقى طبعا ان هو بوفرلي درجة حرارة
+
+213
+00:16:01,370 --> 00:16:05,450
+معينة لأ التفاعل عشان انا اعمل incubation زي تحضين
+
+214
+00:16:05,450 --> 00:16:09,310
+للانابيب على درجة حرارة معينة لحتى يتم التفاعل
+
+215
+00:16:09,310 --> 00:16:16,680
+تمام؟طيب when only a few samples in a tube require
+
+216
+00:16:16,680 --> 00:16:19,800
+incubation it's more convenient and less expensive
+
+217
+00:16:19,800 --> 00:16:23,680
+to use a dry hot heat block طبعا لما بيكون عندى
+
+218
+00:16:23,680 --> 00:16:26,720
+كمية العينة قليلة او المحاليه القليله و الانابيب
+
+219
+00:16:26,720 --> 00:16:29,760
+الصغيرة زى الabendrof بتعرفوا الabendrof التيوب
+
+220
+00:16:29,760 --> 00:16:32,720
+اللى هى الانابيب اللى اعطيتكن فيها عينة ال blood
+
+221
+00:16:32,720 --> 00:16:38,560
+فى المتحان العملي انابيب صغيرة جدا طبعا لو انا ..
+
+222
+00:16:38,710 --> 00:16:42,570
+بشتغل فيها يعني بشتغل في كميات قليلة فلازم أنا
+
+223
+00:16:42,570 --> 00:16:45,590
+أستخدم بدل ما أستخدم ال water bath بستخدم ال heat
+
+224
+00:16:45,590 --> 00:16:50,170
+block تمام؟ يعني هو عبارة عن block معدني بيسخن عن
+
+225
+00:16:50,170 --> 00:16:53,210
+درجة حرارة معينة و بيظل ثابت درجة الحرارة و برضه
+
+226
+00:16:53,210 --> 00:16:55,630
+نفس الاشي بيعملي incubation لإن أنا بيبقى على درجة
+
+227
+00:16:55,630 --> 00:16:59,870
+الحرارة اللي بديها يعني أنا بختارها هذه الدرجةطيب
+
+228
+00:16:59,870 --> 00:17:03,690
+فبالتالي هو عبارة عن ال dry bath اذا هو مش water
+
+229
+00:17:03,690 --> 00:17:06,470
+bath لأ dry bath لأنه زي ما انتوا شايفين مش water
+
+230
+00:17:06,470 --> 00:17:09,970
+في الموضوع و هي block معدني بيسخن و بيبرد حسب درجة
+
+231
+00:17:09,970 --> 00:17:14,070
+الحرارة اللي انا بدي اياها تمام ال chemical test
+
+232
+00:17:14,070 --> 00:17:17,850
+التفاعلات الكيميائية react test at specific
+
+233
+00:17:17,850 --> 00:17:21,730
+temperature طبعا كل تفاعل كيميائي له درجة حرارة
+
+234
+00:17:21,730 --> 00:17:26,470
+خاصة بيشتغل عليها و بيكون افضل reaction او افضل
+
+235
+00:17:26,470 --> 00:17:31,670
+نتيجة على هاي درجة الحرارةmany tests react at room
+
+236
+00:17:31,670 --> 00:17:35,990
+temperature في تفاعلات احنا كنا بيمكن نعمل لهم
+
+237
+00:17:35,990 --> 00:17:40,210
+incubation على درجة حرارة الغرفة من 18 ل 22 مثلا
+
+238
+00:17:40,210 --> 00:17:46,310
+زي ال .. فحص ال homoglobin و فحص ال glucose كنا
+
+239
+00:17:46,310 --> 00:17:50,940
+نستنى 10 دقائق في درجة حرارة الغرفةطيب و لو أنا
+
+240
+00:17:50,940 --> 00:17:55,200
+بدي .. ممكن بقى أحيانا التفاعلات بتكون بتحتاج درجة
+
+241
+00:17:55,200 --> 00:17:59,340
+حرارة 37 أيضا ال homoglobin و ال glucose كان ممكن
+
+242
+00:17:59,340 --> 00:18:01,940
+أن أنا أحطهم على .. في ال water بس على درجة حرارة
+
+243
+00:18:01,940 --> 00:18:08,220
+37 و أستنى خمس دقائق مش عشرة طبعا فإذا ال .. ال
+
+244
+00:18:08,220 --> 00:18:12,220
+incubation time بيختلف من درجة حرارة إلى أخرى و
+
+245
+00:18:12,220 --> 00:18:16,260
+أيضا يعني كل تفاعل له ال bestincubation
+
+246
+00:18:16,260 --> 00:18:21,360
+temperature تمام؟ فأنا بضبط ال .. ال water bath
+
+247
+00:18:21,360 --> 00:18:25,340
+على حسب ال .. الدرجة الحرارة الأفضل للتفاعل اللي
+
+248
+00:18:25,340 --> 00:18:31,540
+أنا عم بشتغلهuse and care of water bath كيف أنا
+
+249
+00:18:31,540 --> 00:18:35,700
+أستخدم جهاز ال water bath و كيف أهتم فيه طبعا زي
+
+250
+00:18:35,700 --> 00:18:38,860
+ما انتوا شايفين read manufacturing instruction هذي
+
+251
+00:18:38,860 --> 00:18:43,140
+هتشوفوها في كل الأجهزة كأول بند ان انا لازم أقرأ
+
+252
+00:18:43,140 --> 00:18:46,420
+تعليمات الجهة المصنعة و ال catalog الخاص بالجهاز
+
+253
+00:18:46,420 --> 00:18:50,120
+لحتى أفهم كل شي بيتعلق فيه تركيبه تشغيله
+
+254
+00:18:50,120 --> 00:18:53,820
+calibration لإله إذا بيحتاج calibration و نصائح
+
+255
+00:18:53,820 --> 00:18:58,440
+أخرىFill the bath and maintain its level with
+
+256
+00:18:58,440 --> 00:19:02,260
+distilled water if unavailable with boiled water
+
+257
+00:19:02,260 --> 00:19:07,320
+طبعا أنا بدي أملأ هذا الجهاز بالـ distilled water،
+
+258
+00:19:07,320 --> 00:19:10,300
+يفضل distilled water عشان ما لو انا بدي استخدم ال
+
+259
+00:19:10,300 --> 00:19:15,690
+tap water، ال tap water فيها أملحو ال .. ممكن ترسب
+
+260
+00:19:15,690 --> 00:19:20,750
+على جدران ال water bath و تؤدي إلى تلفة فبالتالي
+
+261
+00:19:20,750 --> 00:19:23,150
+يفضل إن أنا أستخدم distilled water إذا ما ..
+
+262
+00:19:23,150 --> 00:19:25,750
+ماعندي distilled water متوفر ممكن أستخدم الماء
+
+263
+00:19:25,750 --> 00:19:31,000
+المغلي اللي هو ال boiled waterطيب و طبعا ليش حكينا
+
+264
+00:19:31,000 --> 00:19:34,920
+this is necessary مضروري to minimize salt
+
+265
+00:19:34,920 --> 00:19:40,100
+deposition on the heater عشان ما يقلل ترسيب الأملح
+
+266
+00:19:40,100 --> 00:19:44,680
+في الجهاز to minimize the growth of microorganism
+
+267
+00:19:44,680 --> 00:19:48,940
+طبعا حتى الميه ما تعفن و ما تخضرن و مايصير فيها زي
+
+268
+00:19:48,940 --> 00:19:54,520
+الخضار عفن او زي هيك زي ال ..هي التحالي بيكون فيها
+
+269
+00:19:54,520 --> 00:20:00,040
+لازم انا اضيف مادة معقمة اللي لها disinfectant و
+
+270
+00:20:00,040 --> 00:20:04,220
+bactericidal agent يعني ماء عامل قاتل للبكتيريا
+
+271
+00:20:04,220 --> 00:20:08,100
+اللي هو ال mercellate تمام؟ بضيف للماء اللي انا
+
+272
+00:20:08,100 --> 00:20:12,660
+ضفته في ال water bath ال mercellate طبعا لكل مثلا
+
+273
+00:20:12,660 --> 00:20:18,420
+الف milli يعني لكل one liter مثلا من الماء اللي
+
+274
+00:20:18,420 --> 00:20:27,010
+انا ضفته بحط one ml لكل الف mlتمام بحط one ml من
+
+275
+00:20:27,010 --> 00:20:32,030
+ال mer cellulite يعني ال dilution واحد لأقل before
+
+276
+00:20:32,030 --> 00:20:35,690
+incubating samples ا check the temperature جاب
+
+277
+00:20:35,690 --> 00:20:40,250
+اللي اعمل incubation للعينات لازم اعمل check على
+
+278
+00:20:40,250 --> 00:20:44,330
+ال temperature اشيك عليها و إذا كان محتاجة ان انا
+
+279
+00:20:44,330 --> 00:20:51,080
+مثلايعني أطبطها على درجة حرارة أعلى أو أقل و أيضا
+
+280
+00:20:51,080 --> 00:20:53,560
+ممكن أنا أتأكد من درجة الحرارة باستخدام
+
+281
+00:20:53,560 --> 00:20:59,040
+thermometer طبعا لازم يكون المستوى الماء ال level
+
+282
+00:20:59,040 --> 00:21:02,440
+of water is above the level of water whatever is
+
+283
+00:21:02,440 --> 00:21:06,800
+being incubated يعني معنى هذه الجملة أنه لازم يكون
+
+284
+00:21:06,800 --> 00:21:11,800
+مستوى الماء يغمر الأنابيبالمراد مثلا ال incubation
+
+285
+00:21:11,800 --> 00:21:15,520
+لإلها يعني إذا كان أنا بستخدم أنابيب كبيرة و
+
+286
+00:21:15,520 --> 00:21:18,380
+المحلول اللي فيها عالي بدي أزود نسبة الماء عشان
+
+287
+00:21:18,380 --> 00:21:21,640
+يغمرها عشان يعمل إلها incubation ما بنفعل أنا ..
+
+288
+00:21:21,640 --> 00:21:25,860
+يعني الماء اللي .. اللي في ال incubator أو في ال
+
+289
+00:21:25,860 --> 00:21:29,380
+water bath .. الماء اللي في ال water bath ما بنفع
+
+290
+00:21:29,380 --> 00:21:33,380
+إنه يكون يدوب ملامس ال tube أو مش ملامسها، هي أنا
+
+291
+00:21:33,380 --> 00:21:37,610
+ما بكون عم بعمل incubation، تمام؟raise the
+
+292
+00:21:37,610 --> 00:21:42,750
+temperature to تسعين درجة or higher at once a week
+
+293
+00:21:42,750 --> 00:21:45,690
+for half an hour for the purpose of
+
+294
+00:21:45,690 --> 00:21:49,630
+decontamination بحكيلك مثلا مرة في الإسبوع لمدة
+
+295
+00:21:49,630 --> 00:21:54,230
+مثلا نص ساعة ارفعي درجة الحرارة لتسعين و نزليها
+
+296
+00:21:54,230 --> 00:21:57,610
+تاني او مثلا شغل الجهاز ل .. اضبطيه على تسعيني
+
+297
+00:21:57,610 --> 00:22:01,570
+يسخن الميه و يرجع .. و ارجع يطفي يخليه يبرد تاني
+
+298
+00:22:01,920 --> 00:22:05,780
+ليش؟ هذه درجة الحرارة العالية بتقتل البكتيريا يعني
+
+299
+00:22:05,780 --> 00:22:11,240
+in addition to myrciulate بالإضافة لmyrciulate use
+
+300
+00:22:11,240 --> 00:22:15,160
+the lid to prevent loss of heat from the bath لازم
+
+301
+00:22:15,160 --> 00:22:20,880
+أنا خلال ال .. ال .. ال .. يعني ال incubation time
+
+302
+00:22:20,880 --> 00:22:25,200
+أكون عم بغطي جهاز ال water bath حتى يعني اتضل درجة
+
+303
+00:22:25,200 --> 00:22:29,050
+الحرارة منتظمةما .. ما يبرد الجهاز و يضل يرجع يسخن
+
+304
+00:22:29,050 --> 00:22:34,190
+تاني يعني بضمن ثباتية درجة الحرارة و أيضا حتى ما
+
+305
+00:22:34,190 --> 00:22:37,590
+يتبخر الماء اللي موجود فيها و to minimize
+
+306
+00:22:37,590 --> 00:22:40,550
+particles from entering the water يعني حتى ما
+
+307
+00:22:40,550 --> 00:22:45,870
+يضايق غبار او مواد او اشي ي .. ي .. يعني يقع في
+
+308
+00:22:45,870 --> 00:22:50,550
+الماء و يلوثهwhen removing the lid after
+
+309
+00:22:50,550 --> 00:22:54,570
+incubation take care to avoid any water entering
+
+310
+00:22:54,570 --> 00:23:01,280
+uncapped tubes طبعا لما أنا ب .. بغطي الجهازو
+
+311
+00:23:01,280 --> 00:23:06,180
+برفعه لازم اتأكد انه ال .. القنابيب ما بيفوت فيها
+
+312
+00:23:06,180 --> 00:23:09,060
+البخار الماء اللى بيتساعد و بيتكثف على غطاية
+
+313
+00:23:09,060 --> 00:23:12,680
+الجهاز مايفوت داخل القنابيب اذا لازم انا اكون عم
+
+314
+00:23:12,680 --> 00:23:16,900
+بسكر القنابيب باستخدام الكعب الخاص فيها او
+
+315
+00:23:16,900 --> 00:23:21,120
+باستخدام cotton يعني المهم انه اضمن ان القنابيب
+
+316
+00:23:21,120 --> 00:23:25,960
+مايفوت لمحتوها ماء هيك هيأثر على التركيز اللى عم
+
+317
+00:23:25,960 --> 00:23:29,970
+بشتغل فيها و ايضاإذا أنا بدأ أقرأ شدة لون أو تركيز
+
+318
+00:23:29,970 --> 00:23:35,050
+أو إشي فممكن يأثر على النتيجة clean the water bath
+
+319
+00:23:35,050 --> 00:23:38,690
+regularly طبعا لازم أنا أنظفه باستمرار we take
+
+320
+00:23:38,690 --> 00:23:41,750
+care not to damage the heating unit طبعا بيكون في
+
+321
+00:23:41,750 --> 00:23:50,340
+عندي زي قداء بتسخن زي هيك زيسخان أو زي مادة ..
+
+322
+00:23:50,340 --> 00:23:54,720
+يعني جزء معدني هو اللي بيسخن و بيسخل الميه، تمام؟
+
+323
+00:23:54,720 --> 00:23:59,640
+فهذا الجزء طبعا مش لازم انا احاول .. يعني احاول
+
+324
+00:23:59,640 --> 00:24:04,780
+اتعامل معاه برفق و مش لازم انا اكسره او اطلفه
+
+325
+00:24:05,800 --> 00:24:09,100
+unplug the bath from the wall socket when not
+
+326
+00:24:09,100 --> 00:24:13,380
+using it طبعا مجرد هذا الاشي بيطبق مش بس على ال
+
+327
+00:24:13,380 --> 00:24:18,020
+water bath على كل الأجهزة اللي عم نستخدمها لازم
+
+328
+00:24:18,020 --> 00:24:22,560
+انا مجرد ما اخلص يعني افك الكبش من الحيط يعني من
+
+329
+00:24:22,560 --> 00:24:27,620
+ال .. من ال .. ال .. ال fish نفسها افكها يعني مش
+
+330
+00:24:27,620 --> 00:24:33,400
+لازم اخليه موصل بالكهربا هذا لإنه لحتة أقل منخطر
+
+331
+00:24:33,400 --> 00:24:37,660
+ال .. ال .. الكهربا اللي ممكن يكون موجود في البيئة
+
+332
+00:24:37,660 --> 00:24:41,540
+اللي أنا عم بشتغل فيها every three to six months I
+
+333
+00:24:41,540 --> 00:24:44,640
+check the bath for correction طبعا يعني كل تلت لست
+
+334
+00:24:44,640 --> 00:24:48,480
+شهور بأتأكد من صحة درجة الحرارة اللي بيعطينيها هذا
+
+335
+00:24:48,480 --> 00:24:54,720
+الجهاززي أني مثلا أحط درجة حرارة بشكل random مثلا
+
+336
+00:24:54,720 --> 00:24:59,220
+أربعين درجة أخليه يسخن و يصل لدرجة الأربعين و يثبت
+
+337
+00:24:59,220 --> 00:25:03,200
+عليها بجيب thermometer و بخليه يقيس يشوف لإن ..
+
+338
+00:25:03,200 --> 00:25:06,100
+أتأكد أنه والله ال thermometer بيعطيني أربعين زي
+
+339
+00:25:06,100 --> 00:25:10,140
+ما الجهاز بيعطيني أربعين ولا لأ فهذا يعني بتأكد من
+
+340
+00:25:10,140 --> 00:25:16,760
+صحة درجات الحرارة اللي بيصل لها الجهازIf you are
+
+341
+00:25:16,760 --> 00:25:21,020
+using a boiling water bath and oven, be sure you
+
+342
+00:25:21,020 --> 00:25:25,420
+use heat resistant glass or plasticware طبعا إذا
+
+343
+00:25:25,420 --> 00:25:28,800
+أنا كنت مستخدم سواء كان oven أو حتى water bath
+
+344
+00:25:28,800 --> 00:25:34,160
+ويوصل لدرجة حرارة الغليان لازم ال glass اللي أنا
+
+345
+00:25:34,160 --> 00:25:38,560
+بيه ب اللي بستخدمها أو حتى سواء كانت plastic لازم
+
+346
+00:25:38,560 --> 00:25:42,020
+يكون plastic مقوة بيتحمل درجات حرارة عالية و أيضا
+
+347
+00:25:42,020 --> 00:25:45,680
+نفس الاشي لازم يكون heat resistant glassيعني مثلا
+
+348
+00:25:45,680 --> 00:25:49,800
+يكون ال .. الانابيب الزجاجية مصنوعة من الزجاج ال
+
+349
+00:25:49,800 --> 00:25:54,280
+birex اللي هي أصلا ال .. فعليا هي هذه المادة اللي
+
+350
+00:25:54,280 --> 00:25:58,140
+احنا بنستخدمها في الانابيب الزجاجية اللي بتشتغلوا
+
+351
+00:25:58,140 --> 00:26:03,020
+فيها و في ال flaskات و في ال .. السلندرات و كل إشي
+
+352
+00:26:03,020 --> 00:26:06,020
+زجاجي عندنا من ال birex يعني هو heat resistant
+
+353
+00:26:11,570 --> 00:26:18,730
+طبعا أثناء إدخال وخروج أي أدوات وانابيب من ال
+
+354
+00:26:18,730 --> 00:26:21,110
+incubator إذا أنا عم بشتغل على درجة حرارة عالية
+
+355
+00:26:21,110 --> 00:26:25,590
+لازم أكون مستخدمة كفات خاصة لحتى ما أقدي نفسي أو
+
+356
+00:26:25,590 --> 00:26:31,620
+أحرق نفسيو incubator اللي هو الحاضنة أو الجهاء
+
+357
+00:26:31,620 --> 00:26:35,840
+الأعلى الحاضن أو الحاضن ال incubator طبعا بيعطيني
+
+358
+00:26:35,840 --> 00:26:40,600
+incubation و طبعا على control temperature على درجة
+
+359
+00:26:40,600 --> 00:26:45,320
+حرارة مضبوطةولكن مش لتفاعل هو لأ لل
+
+360
+00:26:45,320 --> 00:26:49,200
+bacteriological culture لشغل مزرعة البكتيريا لما
+
+361
+00:26:49,200 --> 00:26:55,320
+انا بدي اعزل بكتيريا او فطر او يعني ميكروب من عينة
+
+362
+00:26:55,320 --> 00:27:03,140
+مريض طبعا لازم انا احط العينة اضيف العينة تبعته
+
+363
+00:27:03,140 --> 00:27:08,040
+على مادة مغذية تنعش وتثري نمو الميكروب
+
+364
+00:27:11,590 --> 00:27:17,270
+أيضا يعني .. يعني أعمل لها incubation لحتى تنمو
+
+365
+00:27:17,270 --> 00:27:22,610
+على درجة حرارة مثلا 37 ل ال blood transfusion لحفظ
+
+366
+00:27:22,610 --> 00:27:27,790
+وحدات الدم لبعض التفاعلات اللي في قسم السيرولوجي
+
+367
+00:27:27,790 --> 00:27:31,230
+اللي هو الأمصال لقسم ال hematology اللي هو علم
+
+368
+00:27:31,230 --> 00:27:35,310
+الدم لقسم ال chemistryيعني أحيانا مثلا بدي
+
+369
+00:27:35,310 --> 00:27:40,230
+incubation سريع على درجة حرارة 37 بدل ما أستنى لسه
+
+370
+00:27:40,230 --> 00:27:45,110
+بدي أسخن water bath و أصل لدرجة حرارة 37 ال
+
+371
+00:27:45,110 --> 00:27:47,770
+incubator إذا موجود عندكوا already غالبا بيكون
+
+372
+00:27:47,770 --> 00:27:50,810
+مضبوط على درجة حرارة 37 اللي هي درجة حرارة نمو
+
+373
+00:27:50,810 --> 00:27:54,330
+البكتيريا و الفطريات و الميكروبات و في ال .. في ال
+
+374
+00:27:54,330 --> 00:27:58,270
+cultures فبالتالي يعني عادي سريع جدا ممكن أفوت
+
+375
+00:27:58,270 --> 00:28:00,790
+الأنابيب تبعتي أعمللها incubation الوقت اللي بديها
+
+376
+00:28:00,790 --> 00:28:04,500
+و أطلعهافي حالة ان انا لو بشتغل فحوصات chemistry
+
+377
+00:28:04,500 --> 00:28:10,540
+كيميا for bacteriological culture an incubator is
+
+378
+00:28:10,540 --> 00:28:14,980
+required whereas for other tests a dry heat block
+
+379
+00:28:14,980 --> 00:28:22,220
+or water bath may be used حكينا انه ال .. في ال ..
+
+380
+00:28:22,220 --> 00:28:24,920
+في ال bacteriological culture في المزارع البكتيرية
+
+381
+00:28:24,920 --> 00:28:30,130
+احنا بنستخدم ال incubatorلكن في الحصات الأخرى ممكن
+
+382
+00:28:30,130 --> 00:28:35,590
+أستخدم اللي هو ال dry heat block أو ال dry bath
+
+383
+00:28:35,590 --> 00:28:39,310
+incubator أو ال incubator العادي
+
+384
+00:28:41,940 --> 00:28:46,100
+و غالبا بيكون usually على 37 درجة مضبوط و حكينا
+
+385
+00:28:46,100 --> 00:28:49,400
+إنه ممكن أنا أستخدمه لل other test لو أنا بدي درجة
+
+386
+00:28:49,400 --> 00:28:54,360
+حرارة 37 و بشكل سريعي يعني ممكن أستخدمه the
+
+387
+00:28:54,360 --> 00:28:57,500
+appropriate temperature is obtained by means of
+
+388
+00:28:57,500 --> 00:29:01,940
+temperature regulator طبعا بصل لدرجة الحرارة اللي
+
+389
+00:29:01,940 --> 00:29:09,660
+بديها من خلال ال .. regulatorمنظم لدرجة الحرارة
+
+390
+00:29:09,660 --> 00:29:12,720
+بتظهر عندى درجة الحرارة هنا انا لازم اضل اعملها
+
+391
+00:29:12,720 --> 00:29:16,560
+check باستمرار طبعا فى عندى thermostat بنظملى درجة
+
+392
+00:29:16,560 --> 00:29:20,360
+الحرارة بيطلع ليها هنا 37 لازم اضل اشيك عليها
+
+393
+00:29:20,360 --> 00:29:21,100
+باستمرار
+
+394
+00:29:23,590 --> 00:29:27,450
+حتى أتأكد إنها هي 37 يعني إذا زادت درجة الحرارة أو
+
+395
+00:29:27,450 --> 00:29:31,070
+قلت ممكن يصير فيه تأثير على نمو البكتيريا هاي هذه
+
+396
+00:29:31,070 --> 00:29:36,270
+.. هذه أطباق احنا بنستخدمها لزراعة البكتيريا تمام؟
+
+397
+00:29:36,270 --> 00:29:40,680
+أو حتى كمان ممكن لزراعة الفطرياتممكن لل cell
+
+398
+00:29:40,680 --> 00:29:43,980
+culture لزراعة الخلايا سواء كان بشتغل على فيروسات
+
+399
+00:29:43,980 --> 00:29:47,360
+أو بشتغل على خلايا cancer cells مثلا بدي أدرس
+
+400
+00:29:47,360 --> 00:29:50,820
+سلوكها أو بدي أعمل عليها أبحاث خلايا جذعية كلها
+
+401
+00:29:50,820 --> 00:29:54,740
+هذه طبعا لازم تحفظ على درجة حرارة 37 يعني بدي
+
+402
+00:29:54,740 --> 00:29:59,680
+أستخدم incubator طبعا لحتى أعمله use و care أول
+
+403
+00:29:59,680 --> 00:30:04,120
+إشي بدي أتبع و أقرأ بعناية ال manufacturer
+
+404
+00:30:04,120 --> 00:30:10,090
+instructionو طبعا لازم اتأكد ان ال incubator موجود
+
+405
+00:30:10,090 --> 00:30:15,510
+على ال .. ال position on a level surface يكون يعني
+
+406
+00:30:15,510 --> 00:30:21,490
+مستوي على بنش مستوي و مايكون في انسداد لنظام
+
+407
+00:30:21,490 --> 00:30:26,490
+التهوية لإله يعني مايكون خابط في الحيط ورا عاملى
+
+408
+00:30:26,490 --> 00:30:32,540
+block لنظام التهوية لإن هو بياخد هوا و ..بيدفيه
+
+409
+00:30:32,540 --> 00:30:37,620
+لدرجة حرارة 37 و بيخلي الوسط كله هنا 37 و هنا زي
+
+410
+00:30:37,620 --> 00:30:41,460
+ما انتوا شايفين فيه زي thermometer عشان نتأكد ان
+
+411
+00:30:41,460 --> 00:30:44,380
+درجة الحرارة جوا زي اللي موجودة على الشاشة برا
+
+412
+00:30:47,090 --> 00:30:50,610
+طمام if the incubator doesn't have temperature
+
+413
+00:30:50,610 --> 00:30:54,290
+display insert a thermometer لو كان مافيش عندي هنا
+
+414
+00:30:54,290 --> 00:31:01,430
+شاشة لحتى تبينلي درجة الحرارة ممكن انا احط
+
+415
+00:31:01,430 --> 00:31:05,020
+thermometer في الداخل و اضل اراقبهbefore
+
+416
+00:31:05,020 --> 00:31:07,280
+incubating culture and test it check the
+
+417
+00:31:07,280 --> 00:31:10,920
+temperature حكينا قبل ال incubation لازم أشيك على
+
+418
+00:31:10,920 --> 00:31:16,240
+درجة الحرارة لازم أنضفه باستمرار يعني بعد ما أقرأ
+
+419
+00:31:16,240 --> 00:31:20,260
+الأطباق هاي و أخلص استخدامتيها لازم أطلعها على
+
+420
+00:31:20,260 --> 00:31:24,990
+التعقيماعدام يعني بتروح اعدام على جهاز التعقيم
+
+421
+00:31:24,990 --> 00:31:28,530
+بنقتل كل البكتيريا و الميكروبات اللى موجودة لضمان
+
+422
+00:31:28,530 --> 00:31:35,370
+عدم نقل العدوى او نقل الجراثيم و .. و بنضف الجهاز
+
+423
+00:31:35,370 --> 00:31:41,130
+باستمرار لحتى انا اضل محافظة عليه نظيف و لضمان
+
+424
+00:31:41,130 --> 00:31:42,150
+سلامتى كمان
+
+425
+00:31:46,160 --> 00:31:51,780
+طبعا و أنا بنطفه لازم أتأكد أن هو مفصول من الكهربا
+
+426
+00:31:51,780 --> 00:31:55,180
+علشان أبقى محافظة على نفسي Instruments for
+
+427
+00:31:55,180 --> 00:32:00,360
+purifying water اللي هو عبارة عن أجهزة لطنقية
+
+428
+00:32:00,360 --> 00:32:05,780
+الماء تمام؟ في عندي أول حاجة طبعا جودة الماء مهمة
+
+429
+00:32:05,780 --> 00:32:11,140
+جدا is very crucial أساسية جدا ليش؟ لأن الماء هو
+
+430
+00:32:11,140 --> 00:32:16,250
+the most common solventused in medical
+
+431
+00:32:16,250 --> 00:32:20,050
+laboratories هو يعني أنا بدأ أحضر saline بدوّب ملح
+
+432
+00:32:20,050 --> 00:32:23,350
+في distilled water لو بدأ أحضر سبغة بدوّب بال
+
+433
+00:32:23,350 --> 00:32:27,150
+powder تبع السبغة بدistilled water لو بدأ أحضر
+
+434
+00:32:27,150 --> 00:32:31,150
+buffer بدي أدوّب ال base و ال acid بدistilled
+
+435
+00:32:31,150 --> 00:32:34,570
+water لو بدي أحضر reagent حكيتلكم ال reagent ممكن
+
+436
+00:32:34,570 --> 00:32:37,910
+ييجي عبارة عن powder و أدوبه في distilled waterإذا
+
+437
+00:32:37,910 --> 00:32:41,630
+لو بدي عندي محلول مركز و بدي أخففه بقى أخفف ب
+
+438
+00:32:41,630 --> 00:32:45,310
+distilled water إذا ال water هو ال most common
+
+439
+00:32:45,310 --> 00:32:50,790
+solvent هو ال solvent أو المضيب اللي هو اللي أكتر
+
+440
+00:32:50,790 --> 00:32:55,790
+شيوعا أو أكتر استخداما بال medical laboratories
+
+441
+00:32:56,210 --> 00:33:00,190
+طبعا بيستخدم في تحضير ال standard solutions لل
+
+442
+00:33:00,190 --> 00:33:05,830
+buffers لل controls طبعا مثلا ال standard solution
+
+443
+00:33:05,830 --> 00:33:13,290
+اللي تركيزه كان 40x في فحص السكر طبعا كان ..
+
+444
+00:33:13,290 --> 00:33:20,060
+تركيزه 400مش اربعين extra اربعمية طبعا و انا لازم
+
+445
+00:33:20,060 --> 00:33:23,700
+اوصله لمية اللي هو ال working solution ال working
+
+446
+00:33:23,700 --> 00:33:29,200
+standard solution لما بدي استخدمه لازم اخففه ب ..
+
+447
+00:33:29,200 --> 00:33:33,010
+ب distilled water طبعاال buffers أيضا تحضيرها
+
+448
+00:33:33,010 --> 00:33:39,910
+حكينا ال controls اللي هي العينات الضابطة الصبغات
+
+449
+00:33:39,910 --> 00:33:43,990
+المحالي لل reagents في سواء كان في قسم ال
+
+450
+00:33:43,990 --> 00:33:48,230
+chemistry في المناعة في ال hematologyفي ال
+
+451
+00:33:48,230 --> 00:33:53,010
+microbiology اللي هو علم الميكروبات والجرافيم في
+
+452
+00:33:53,010 --> 00:33:57,650
+كل الأقسام أنا بستخدم distilled water ك .. ك ..
+
+453
+00:33:57,650 --> 00:34:02,490
+يعني مثيب أو solution لتحضير أو كبيس كاساس يعني
+
+454
+00:34:02,940 --> 00:34:06,860
+لتحضير ال reagents طبعا و ايضا كمان ال culture
+
+455
+00:34:06,860 --> 00:34:11,700
+media هي المادة المغذية اللى بتنمي البكتيريا و
+
+456
+00:34:11,700 --> 00:34:16,840
+الفطريات و لزراعة الخلايا ايضا بتكون برضه كمان
+
+457
+00:34:16,840 --> 00:34:20,140
+عبارة عن مواد مغذية in distilled water يعني لما
+
+458
+00:34:20,140 --> 00:34:23,960
+بدى احضر media للبكتيريا ولا للفطريات بوزن ال ..
+
+459
+00:34:23,960 --> 00:34:27,340
+البودر و بدوبه في distilled water بفوته يتعقم و
+
+460
+00:34:27,340 --> 00:34:33,860
+بعدين بفضيه في أطباق علشان أزرع عليها العيناتتمام؟
+
+461
+00:34:33,860 --> 00:34:40,160
+فال .. ال reagent اللي بتستخدم في ال blood is
+
+462
+00:34:40,160 --> 00:34:44,400
+transfusion work فشغل اللي هو نقل الدم برضه كمان
+
+463
+00:34:44,400 --> 00:34:49,320
+محتاجة distilled water فبالتالي إذا ال water يعني
+
+464
+00:34:49,320 --> 00:34:56,340
+يستخدم فيه كتير مجالات في حال .. أحيانا بالأجهزةلو
+
+465
+00:34:56,340 --> 00:35:01,660
+بدي أنظف تيوب بده .. أحيانا في بعض الأجهزة خصوصا
+
+466
+00:35:01,660 --> 00:35:05,420
+الأجهزة اللي بتسحب العينة سحب لازم ال .. ده اللي
+
+467
+00:35:05,420 --> 00:35:10,060
+بتسحب اللي فيها لازم يسيللها rinsing أشرنزنج يعني
+
+468
+00:35:10,060 --> 00:35:13,560
+غمر بغسول أو بمضيب يعني ان احنا ننظفها ما بين كل
+
+469
+00:35:13,560 --> 00:35:19,320
+عينة و التانيةأيضا نحتاج ال .. ال water شايفين كام
+
+470
+00:35:19,320 --> 00:35:24,040
+هي مهمة ال water؟ فلازم أيضا الأجهزة اللي ت .. ال
+
+471
+00:35:24,040 --> 00:35:30,720
+.. التحضير و الفصل و التعقيم تكون مهمة في عندي
+
+472
+00:35:30,720 --> 00:35:34,220
+جهاز اللي هو ال water distilling apparatus اللي هو
+
+473
+00:35:34,220 --> 00:35:41,300
+يعني فكرة ال .. ال .. الفلترة التنقية و الفلترة
+
+474
+00:35:41,300 --> 00:35:47,570
+للماءطبعا هي فكرة بسيطة بتعتمد على تسخين الماء و
+
+475
+00:35:47,570 --> 00:35:52,170
+.. فبالتالي ال .. ال .. ال .. يعني هو الماء غير
+
+476
+00:35:52,170 --> 00:35:57,650
+نقيب بيتعرض لحرارة بيسخن البخار بيتكاثف و بيتم
+
+477
+00:35:57,650 --> 00:36:03,250
+يعني نقله من خ .. لما بيتكاثف بيتم نقله إلى وعاء
+
+478
+00:36:03,250 --> 00:36:12,470
+نظيف فال .. ال ..هذا الماء المتكاثف طبعا هو اللي
+
+479
+00:36:12,470 --> 00:36:14,810
+.. اللي بنعتبره distilled water لإنه خالي من
+
+480
+00:36:14,810 --> 00:36:19,190
+الشوائب و الأملح تمام؟ لإنه لما اتبخر ما اتبخرت
+
+481
+00:36:19,190 --> 00:36:22,970
+معاه الأملح و الشوائب اللي موجودة فيه و لما تكاثف
+
+482
+00:36:22,970 --> 00:36:28,270
+نتيجة يعني وجوده في .. على سطح .. على أسطح باردة
+
+483
+00:36:28,270 --> 00:36:33,230
+تمام؟ يعني ماكان موجود فيه هاي الشوائب لذلك هيك
+
+484
+00:36:33,230 --> 00:36:37,650
+بنكون احنا حصلنا على distilled water تمام؟إذن
+
+485
+00:36:37,650 --> 00:36:42,230
+water distilling apparatus is an instrument that
+
+486
+00:36:42,230 --> 00:36:46,890
+used to purify impure water هو عبارة عن جهاز
+
+487
+00:36:46,890 --> 00:36:52,910
+لتنقية ال .. to purify تنقية ال impure water اللي
+
+488
+00:36:52,910 --> 00:36:56,370
+هو الماء الغير نقي by process known as
+
+489
+00:36:56,370 --> 00:36:59,770
+distillation من خلال عملية اسمها distillation اللي
+
+490
+00:36:59,770 --> 00:37:03,980
+احنا شرحنا مبدأها حالياdistillation هو عبارة عن
+
+491
+00:37:03,980 --> 00:37:07,420
+process by which impure water is boiled، بنغلي
+
+492
+00:37:07,420 --> 00:37:12,320
+الميه اللى هى ال impure، اللى هى الغير نقية، تمام؟
+
+493
+00:37:12,320 --> 00:37:17,120
+و بخارها بيتم نقله الى أنابيب أخرى، تمام؟ هذه
+
+494
+00:37:17,120 --> 00:37:19,980
+الأنابيب مكثفة، ليش؟ لإنها بتكون طبعا ال ..
+
+495
+00:37:23,380 --> 00:37:30,340
+البخار هذا بيكون موجود في أنابيب باردة نوعا ما
+
+496
+00:37:30,340 --> 00:37:35,600
+فبيتكاثف وبيتم نقله ك pure water تمام؟ even the
+
+497
+00:37:35,600 --> 00:37:38,900
+steam produced condensed in a cold surface بيتم
+
+498
+00:37:38,900 --> 00:37:43,220
+تكاثفه على أسطح باردة اللي هي الcondenser وطبعا
+
+499
+00:37:43,220 --> 00:37:47,120
+بيعطيني chemically pure distilled waterيعني ماء
+
+500
+00:37:47,120 --> 00:37:52,840
+خالي من الشوائب الكيميائية طبعا that is water from
+
+501
+00:37:52,840 --> 00:37:56,920
+which non-volatile organic and inorganic materials
+
+502
+00:37:56,920 --> 00:38:01,320
+are removed طبعا المواد الغير متطايرة اللي موجودة
+
+503
+00:38:01,320 --> 00:38:04,380
+سواء كانت عضوية أو غير عضوية هيك بنكون اتخلصنا
+
+504
+00:38:04,380 --> 00:38:11,150
+منها تمام؟من مساوئ هذه الطريقة إن هي ما بتخلصني من
+
+505
+00:38:11,150 --> 00:38:15,150
+ال ionic gases اللي موجودة في الماء زي إذا كان
+
+506
+00:38:15,150 --> 00:38:18,010
+موجود في ammonia أو carbon dioxide أو chlorine
+
+507
+00:38:18,010 --> 00:38:25,810
+طبعا ما بتخلصني منها و أيضا أحيانا ممكنتكون الماء
+
+508
+00:38:25,810 --> 00:38:28,050
+اللي هي ال distilled water اللي انا حصلت عليها
+
+509
+00:38:28,050 --> 00:38:30,650
+contaminated with non volatile impurities يعني
+
+510
+00:38:30,650 --> 00:38:36,430
+يكون فيها بعض الشوائب الغير متطايرة زي ال sodium و
+
+511
+00:38:36,430 --> 00:38:38,850
+ال potassium و ال calcium و ال carbonate ion و ال
+
+512
+00:38:38,850 --> 00:38:43,930
+sulphate ion طبعا فلذلك ممكن انا استخدم اذا بدي
+
+513
+00:38:43,930 --> 00:38:47,910
+يعني ماء درجة نقاطه اعلى بكتير ممكن استخدم اجهزة
+
+514
+00:38:47,910 --> 00:38:51,410
+تانية غير ال distillator في عندي جهاز اللي هو ال
+
+515
+00:38:51,410 --> 00:38:58,670
+gravity water filterال filtration هو عبارة عن the
+
+516
+00:38:58,670 --> 00:39:02,970
+passage of liquid مرور الماء أو السائل من خلال
+
+517
+00:39:02,970 --> 00:39:07,630
+filter وبيتضمن مروره طبعا لازم من خلال gravity
+
+518
+00:39:07,630 --> 00:39:13,330
+جاذبية أو حتى pressure أو ضغط يعني تمام؟ فبالتالي
+
+519
+00:39:13,330 --> 00:39:21,010
+بيت filter و بيتم ..يعني حصول على الماء نقي نوعا
+
+520
+00:39:21,010 --> 00:39:24,710
+ما طيب هنا زي مثلا هذا الجهاز ال gravity water
+
+521
+00:39:24,710 --> 00:39:28,730
+filter طبعا هذا inlet يعني من هنا بيدخل الماء
+
+522
+00:39:28,730 --> 00:39:34,530
+الغير نقي تمام او المراد فلترته و بيمر على فلاتر
+
+523
+00:39:34,530 --> 00:39:37,610
+يعني الفلاتر هذه تتبين في أحجامها يعني مثلا هنا في
+
+524
+00:39:37,610 --> 00:39:42,260
+عندي سخوريعني المسافات البينية كبيرة بعدين بييجي
+
+525
+00:39:42,260 --> 00:39:47,000
+هنا في عندي perforated metal plate يعني مادة
+
+526
+00:39:47,000 --> 00:39:51,620
+معدنية مثقبة بيمر من خلالها الماء بيمر على ال ..
+
+527
+00:39:51,620 --> 00:39:56,620
+على رمل تمام و احنا بنعرف ان الرمل يستخدم ك filter
+
+528
+00:39:56,620 --> 00:40:00,320
+يعني الماء اللي .. اللي بيطلع من الينابيع من الرمل
+
+529
+00:40:00,320 --> 00:40:07,400
+بيكون كتير نقي لإن الرمل ممكن يشغل ك filter تمام؟
+
+530
+00:40:08,750 --> 00:40:12,770
+فهنا بالنهاية بيطلع عندي .. بيبدأ يرتفع منسوب ال
+
+531
+00:40:12,770 --> 00:40:17,790
+.. ال water كل ما ضغطنا ماء أكتر، تمام؟ بيدخل
+
+532
+00:40:17,790 --> 00:40:20,970
+الماء و بنضغط عليه إما ب .. يعني بإما بينزل بفعل
+
+533
+00:40:20,970 --> 00:40:23,930
+الجاذبية أو أنا ب .. بعمل apply pressure يعني
+
+534
+00:40:23,930 --> 00:40:27,250
+بيكون في عندي ضغط على ال ..المقفة بيرتفع في ..
+
+535
+00:40:27,250 --> 00:40:30,890
+بيبدأ يطلع، هي عبر من خلال ال rock، من خلال ال ..
+
+536
+00:40:30,890 --> 00:40:34,470
+ال .. المعدن، من خلال ال sand و بيبدأ يرتفع كفي
+
+537
+00:40:34,470 --> 00:40:41,590
+يعني يرشح على سطح ال sand كماء مفلتر أو يعني منقه
+
+538
+00:40:41,590 --> 00:40:45,340
+و بعد هيك بيخرج من خلال ال outlet، تمام؟ال
+
+539
+00:40:45,340 --> 00:40:48,640
+filtrate هذا طبعا ال .. ال .. ال .. ناتج الفلترة
+
+540
+00:40:48,640 --> 00:40:52,780
+بسميه filtrate، طبعا؟ وهذا بدي .. المصطلح هتستخدمه
+
+541
+00:40:52,780 --> 00:40:58,580
+كتير في التحليل، يعني هن .. هن filter محليل ون
+
+542
+00:40:58,580 --> 00:41:02,320
+filter ميه ون filter صبغات ون filter أشياء كتير،
+
+543
+00:41:02,320 --> 00:41:07,300
+فالناتج الفلترة بسميه filtrate هو عبارة عن ال
+
+544
+00:41:07,300 --> 00:41:12,440
+liquid that has passed through the filterإذا
+
+545
+00:41:12,440 --> 00:41:17,760
+المحلول اللي خرجوا أو عدّى من خلال ال filter بسميه
+
+546
+00:41:17,760 --> 00:41:22,000
+filtrate the purpose of filtration طبعا إيش وظيفة
+
+547
+00:41:22,000 --> 00:41:25,000
+ال filtration؟ to remove particulate matter يعني
+
+548
+00:41:25,000 --> 00:41:30,570
+لحتى يعني أتخلص من الشوائب و المواد الكبيرةنوعا ما
+
+549
+00:41:30,570 --> 00:41:35,690
+زي ال bacteria و ال parasite و ال microorganism زي
+
+550
+00:41:35,690 --> 00:41:40,470
+لو ماكان فيه شوائب أو يعني أوساخ في داخل الماء
+
+551
+00:41:40,470 --> 00:41:45,630
+ممكن أتخلص منها بهذا الشكل لكن مابتخلصش من خلالمن
+
+552
+00:41:45,630 --> 00:41:49,350
+خلال هذه العملية من ال dissolve salt يعني الأملاح
+
+553
+00:41:49,350 --> 00:41:53,070
+اللي دائبة تمام؟ يعني ممكن أتخلص من بعض الأملاح من
+
+554
+00:41:53,070 --> 00:41:57,090
+خلال مثلا إما ال distillation .. عملية ال
+
+555
+00:41:57,090 --> 00:42:00,990
+distillation أو من خلال اللي هي ال de-ionization
+
+556
+00:42:00,990 --> 00:42:05,750
+تمام؟طيب جديد بالذكر أنه أنا حكيتلكم مثلا إذا كان
+
+557
+00:42:05,750 --> 00:42:11,710
+أنا محتاجة تنقي أكثر ممكن أستخدم أجهزة بتنقي أكثر
+
+558
+00:42:11,710 --> 00:42:16,270
+و أحيانا ممكن أنا أحصل على يعني لحتى أضمن أنه
+
+559
+00:42:16,270 --> 00:42:22,000
+الماء مفلتر جيداأعمل double distillation و كتير
+
+560
+00:42:22,000 --> 00:42:25,460
+أحيانا بنحتاج ال double distilled water يعني ماء
+
+561
+00:42:25,460 --> 00:42:29,800
+مقطر مرتين يعني الماء المقطر اللي هنا أنا رجعت
+
+562
+00:42:29,800 --> 00:42:34,040
+غليته مرتانة و إكثفته، تمام؟ هذا بسميه double
+
+563
+00:42:34,040 --> 00:42:38,600
+distilled water خصوصا في الفحوصات اللي هي ال .. ال
+
+564
+00:42:38,600 --> 00:42:43,940
+molecular في فحوصات ال molecular اللي هي الفحوصات
+
+565
+00:42:43,940 --> 00:42:48,370
+الجينية أنا أحيانا بشتغل بإنزيماتأي تركيز لأملاح
+
+566
+00:42:48,370 --> 00:42:52,330
+أو معادن في داخل الميه ممكن يأثر على تنشاط هذه
+
+567
+00:42:52,330 --> 00:42:57,390
+الانزيمات فبالتالي بكون محتاجة double distilled
+
+568
+00:42:57,390 --> 00:43:04,350
+waterطب ال deionizer إيش يعني نازع للأيونات؟ دي
+
+569
+00:43:04,350 --> 00:43:10,710
+نازع، تمام؟ ionizer يعني نازع للأيونات، تمام؟
+
+570
+00:43:10,710 --> 00:43:16,390
+deionizer هو عبارة عن apparatus to produce ion
+
+571
+00:43:16,390 --> 00:43:21,510
+-free water، تمام؟ لحتى تنتجلي ماء خالي أو يعني
+
+572
+00:43:21,510 --> 00:43:27,570
+free of ions، تمام؟ إذا عملية ال deionization
+
+573
+00:43:30,320 --> 00:43:35,720
+هنا deionization تمام هي عملية is a process in
+
+574
+00:43:35,720 --> 00:43:40,400
+which chemically impure water الماء اللي غير نقي
+
+575
+00:43:40,400 --> 00:43:46,760
+فيه شوائب كيميائية تمام بيمر على anion and cation
+
+576
+00:43:46,760 --> 00:43:51,740
+risen تمام و بالنهاية أنا بحصل على ion-free water
+
+577
+00:43:51,740 --> 00:43:56,760
+إيش يعني anion and cation risen يعني عبارة عن ..
+
+578
+00:43:56,760 --> 00:44:02,310
+يعني زي أقطابفيها انيونات وفيها كاتيونات، تمام؟
+
+579
+00:44:02,310 --> 00:44:06,370
+هلأ لما .. بس نشوف الصورة هنوضح أكتر أو هتتضح
+
+580
+00:44:06,370 --> 00:44:12,010
+الفكرة أكتر Deionized water has low electrical
+
+581
+00:44:12,010 --> 00:44:17,500
+conductivityقليلة أو شبه معضومة ليش؟ لإن إحنا
+
+582
+00:44:17,500 --> 00:44:20,460
+نزعنا الأيونات اللي فيها هو إيش اللي بيخلي الماء
+
+583
+00:44:20,460 --> 00:44:23,740
+وموصل للكهربا غير الأيونات اللي بتكون موجودة فيه
+
+584
+00:44:23,740 --> 00:44:31,530
+near neutral pH بيكون ال pH متعادل about 7و طبعا
+
+585
+00:44:31,530 --> 00:44:35,210
+free from water soluble salt بيكون خالي من الأملاح
+
+586
+00:44:35,210 --> 00:44:39,310
+و خالي من الأيونات بس ممكن ما بيكون معقّم تمام أنا
+
+587
+00:44:39,310 --> 00:44:42,730
+بحتاج أفوته جهاز تعقيم عشان أعقمه بعد ما أنزع
+
+588
+00:44:42,730 --> 00:44:46,330
+الأيونات منه لو أنا محتاجة sterile and deionized
+
+589
+00:44:46,330 --> 00:44:50,250
+water لكن إذا محتاجة deionized water لحال فخلاص
+
+590
+00:44:50,250 --> 00:44:55,510
+بمرره من خلال جهاز ال deionizer و بعدين بستخدمه
+
+591
+00:44:55,510 --> 00:45:02,070
+طيبال cations اللي بتكون ممكن تكون موجودة في الماء
+
+592
+00:45:02,070 --> 00:45:07,890
+which may be present in water زي ash يعني أجزاء
+
+593
+00:45:07,890 --> 00:45:11,910
+مشحونة موجبة cations يعني هتكون زي ال calcium، ال
+
+594
+00:45:11,910 --> 00:45:16,580
+magnesium، ال sodium، تمام؟و هذه أيضًا بيتم ..
+
+595
+00:45:16,580 --> 00:45:20,260
+يعني بيه .. احنا حكينا هتمر على كاتيون و أنيون
+
+596
+00:45:20,260 --> 00:45:25,720
+risen تمام؟ فهتمر على الكاتيون و ياخدها الكاتيون
+
+597
+00:45:25,720 --> 00:45:31,070
+تبع الجهاز و يقطي الماء كاتيون آخروال onions اللى
+
+598
+00:45:31,070 --> 00:45:34,790
+بتكون فى الماء زى الصلفات و ال bicarbonate و ال
+
+599
+00:45:34,790 --> 00:45:38,650
+silicate تمام و ال nitrate و ال chloride أي أجزاء
+
+600
+00:45:38,650 --> 00:45:42,910
+شحنتها سالبة بيتم استبدالها بانيون أخرى من الجهاز
+
+601
+00:45:42,910 --> 00:45:47,730
+يعنى مثلا هى هنا الجهاز موجود فى cation و موجود فى
+
+602
+00:45:47,730 --> 00:45:51,550
+anion resin ال cation resin بيكون موجود فى فقط
+
+603
+00:45:51,550 --> 00:45:55,170
+أيودات ال hydrogen تمام ال protonate يعنى بيكون
+
+604
+00:45:55,170 --> 00:45:59,520
+موجود فىهو ال .. ال .. ال anion resin تبع الجهاز
+
+605
+00:45:59,520 --> 00:46:04,500
+بيكون فيه OH، تمام؟ بيكون فيه OH، هلأ مجرد ما أنا
+
+606
+00:46:04,500 --> 00:46:07,960
+أمرر الماء، هي قطرة الماء اللي مرت مثلا، بنعتبر
+
+607
+00:46:07,960 --> 00:46:10,940
+إنه هذه كمية الماء اللي أنا بدي أعمل لها de
+
+608
+00:46:10,940 --> 00:46:14,180
+-ionization، هلأ هي موجود فيها أنيونات زي ال
+
+609
+00:46:14,180 --> 00:46:17,760
+sodium و موجود فيها .. sorry موجود فيها أنيونات زي
+
+610
+00:46:17,760 --> 00:46:22,640
+ال chloride و كتيونات زي ال sodiumطبعا طيب مرت على
+
+611
+00:46:22,640 --> 00:46:26,660
+ال reason تبع الجهاز اللي هو عبارة عن إيش كتيوناتو
+
+612
+00:46:26,660 --> 00:46:32,280
+هيدروجين و أنيوناتو هيدروكسل طبعا فإيش هيعمل
+
+613
+00:46:32,280 --> 00:46:37,760
+الجهاز؟ هيروح ماخد الصوديوم من الماء ومعطيها بداله
+
+614
+00:46:37,760 --> 00:46:44,220
+اللي هو H plusطبعا استبدال كتاين بكتاين أخد صوديوم
+
+615
+00:46:44,220 --> 00:46:49,120
+و أعطى الماء اتش طبعا فهنا إيش حكينا الكتاين زى
+
+616
+00:46:49,120 --> 00:46:53,020
+اللى فى الجهاز اللى زى الصوديوم صار إلها exchange
+
+617
+00:46:53,020 --> 00:46:58,840
+تم تبديلها بإيش بكتاين من وين من الرزن من الجهاز
+
+618
+00:46:58,840 --> 00:47:06,160
+اللى عبارة عن hydrogen ions طبعا طيبوالجهاز بياخد
+
+619
+00:47:06,160 --> 00:47:11,220
+ال anions اللي هي زي ال chloride المشحونة سالبة و
+
+620
+00:47:11,220 --> 00:47:16,780
+بيروح بيعطي الماء اش بدالها OH برضه مشحونة سالبة،
+
+621
+00:47:16,780 --> 00:47:21,480
+اذا اللي هي ال anions اللي موجودة في الماء زي مين؟
+
+622
+00:47:21,480 --> 00:47:28,060
+زي ال chlorideتمام؟ بيصيرلها exchange بإيش؟
+
+623
+00:47:28,060 --> 00:47:35,440
+بالانيون تبع الرزن تبع الجهاز تمام؟ و ال .. ال ..
+
+624
+00:47:35,440 --> 00:47:37,060
+ال .. ال .. ال .. ال .. ال .. ال .. ال .. ال .. ال
+
+625
+00:47:37,060 --> 00:47:37,080
+.. ال .. ال .. ال .. ال .. ال .. ال .. ال .. ال ..
+
+626
+00:47:37,080 --> 00:47:37,240
+ال .. ال .. ال .. ال .. ال .. ال .. ال .. ال .. ال
+
+627
+00:47:37,240 --> 00:47:37,520
+.. ال .. ال .. ال .. ال .. ال .. ال .. ال .. ال ..
+
+628
+00:47:37,520 --> 00:47:37,920
+ال .. ال .. ال .. ال .. ال .. ال .. ال .. ال .. ال
+
+629
+00:47:37,920 --> 00:47:38,820
+.. ال .. ال .. ال .. ال .. ال .. ال .. ال .. ال ..
+
+630
+00:47:38,820 --> 00:47:39,260
+ال .. ال .. ال .. ال .. ال .. ال .. ال .. ال .. ال
+
+631
+00:47:39,260 --> 00:47:39,340
+.. ال .. ال .. ال .. ال .. ال .. ال .. ال .. ال ..
+
+632
+00:47:39,340 --> 00:47:39,460
+ال .. ال .. ال .. ال .. ال .. ال .. ال .. ال .. ال
+
+633
+00:47:39,460 --> 00:47:39,520
+.. ال .. ال .. ال .. ال .. ال .. ال .. ال .. ال ..
+
+634
+00:47:39,520 --> 00:47:45,420
+ال .. ال .. ال .. ال .. ال .. ال .. ال .. الفقد ال
+
+635
+00:47:45,420 --> 00:47:50,720
+sodium و فقد ال chloride كل منهم راح على ال resin
+
+636
+00:47:50,720 --> 00:47:56,140
+اللي خاص بشحنته تمام؟ و اكتسب بدلهم HOH طب ما هدول
+
+637
+00:47:56,140 --> 00:48:00,200
+ال HOH بيصيروا لهم combine مع بعض، اذا ال hydrogen
+
+638
+00:48:00,200 --> 00:48:03,920
+ion و ال .. و ال hydroxyl بيصيروا لهم combine مع
+
+639
+00:48:03,920 --> 00:48:08,360
+بعض و بيعطونيwater إذا في النهاية أنا حصلت على
+
+640
+00:48:08,360 --> 00:48:13,540
+water بطريقة استبدال الأيونات وخالية من الأيونات
+
+641
+00:48:13,540 --> 00:48:16,980
+الأصلية اللي كانت موجودة في ال water الأصلي، تمام؟
+
+642
+00:48:16,980 --> 00:48:20,580
+هذا ال water اللي أنا حصلت عليه هو عبارة عن de
+
+643
+00:48:20,580 --> 00:48:26,610
+-ionized water، ماء منزوع الأيونات، تمام؟resin إيش
+
+644
+00:48:26,610 --> 00:48:31,070
+معناه كلمة resin؟ هي particles أجزاء على ال .. في
+
+645
+00:48:31,070 --> 00:48:35,710
+ال .. يعني أجزاء بتكون قادرة إنها تحمل شحنة طبعا؟
+
+646
+00:48:35,710 --> 00:48:40,250
+مواد يعني قادرة إنها تحمل شحنة أو تحمل آيونات فهي
+
+647
+00:48:40,250 --> 00:48:45,710
+مواد بنسميها resin مادة بتحمل على سطحها البروتونات
+
+648
+00:48:45,710 --> 00:48:48,830
+هذه أو ال hydrogen ion في حالة .. فبنسميها
+
+649
+00:48:48,830 --> 00:48:53,150
+cationic resin طبعا؟ particle بتكون شايلة ال
+
+650
+00:48:53,150 --> 00:48:58,400
+hydroxylIon تمام فبسميها اللي هو ال anionic rhythm
+
+651
+00:48:58,400 --> 00:49:03,160
+أو ممكن أسميها electrode تمام يعني مثلا هنا .. هاي
+
+652
+00:49:03,160 --> 00:49:06,420
+هنا مثلا ال .. ال .. في ناحية ال positive و هدا من
+
+653
+00:49:06,420 --> 00:49:10,330
+ناحية ال negative يعني هاد ال cationicتمام؟ وهذا
+
+654
+00:49:10,330 --> 00:49:14,750
+ال onionic فاش اللي هيصير بالظبط الماء هيفوت هيروح
+
+655
+00:49:14,750 --> 00:49:18,350
+مثلا ال sodium باتجاه من .. باتجاه ال cationic
+
+656
+00:49:18,350 --> 00:49:22,310
+ويجي بدله hydrogen و ال chloride اللي في الماء
+
+657
+00:49:22,310 --> 00:49:27,970
+مثلا او اي onions موجودة شحنتها سالبة هتروح باتجاه
+
+658
+00:49:27,970 --> 00:49:33,450
+السالب ويتم استبدالها بسالب بدالها تمام؟عشان ما
+
+659
+00:49:33,450 --> 00:49:39,050
+يأثر على شحنة الماء ككل ففي الت .. بالتالي او ..
+
+660
+00:49:39,050 --> 00:49:42,170
+او sorry يعني هو في النهاية احنا معنين انه شحنة ال
+
+661
+00:49:42,170 --> 00:49:47,270
+electrode نفسه ما تتغير ككل طبعا ففي النهاية بيتم
+
+662
+00:49:47,270 --> 00:49:51,350
+نزع ال sodium و ال chloride ب .. باستخدام ال resin
+
+663
+00:49:51,350 --> 00:49:55,670
+هدول واستبدالهم ب hydrogen و hydroxyl و هدول
+
+664
+00:49:55,670 --> 00:49:59,470
+بيعطونى مية اذا انا حصلت على ماء خالي من الايونار
+
+665
+00:49:59,470 --> 00:50:03,290
+بهذه الفكرةتمام؟ هذه فكرة عمل ال .. ال .. ال
+
+666
+00:50:03,290 --> 00:50:12,330
+ionizer هيك بنكون وصلنا لنهاية المحاضرة و أيضا
+
+667
+00:50:12,330 --> 00:50:18,690
+لختم محاضرات مساق المقدمة في التحاليل الطبية أتمنى
+
+668
+00:50:18,690 --> 00:50:24,690
+أنكم تكونوا استفدتم و استمتعتم أتمنى ذلك انه في ..
+
+669
+00:50:24,690 --> 00:50:30,130
+بالمساق و ..ان شاء الله يعني بتطلعوا منه بتكونوا
+
+670
+00:50:30,130 --> 00:50:35,290
+على خبرة و دراية كافية اتمكنكم من انكم تاخدوا
+
+671
+00:50:35,290 --> 00:50:39,870
+المثاقات الأخرى اللى بتعتمد على هذا المثاق و اللى
+
+672
+00:50:39,870 --> 00:50:43,610
+من خلاله أيضا يعني انتوا هتنطلقوا الى التخصصات
+
+673
+00:50:43,610 --> 00:50:47,830
+الأخرى اللى موجودة تحت مسمة علوم طبية مخبرية
+
+674
+00:50:50,320 --> 00:50:57,220
+رح أطالب م .. أطالبكم بواجب أنه تختاري جهاز يستخدم
+
+675
+00:50:57,220 --> 00:51:03,740
+بال medical laboratories، بالمختبرات الطبية، طبعا
+
+676
+00:51:03,740 --> 00:51:07,230
+الجهاز سواء كان incubator، ovenbalance,
+
+677
+00:51:08,170 --> 00:51:13,750
+centrifuge, spectrohotometer او حتى اي جزء اخر
+
+678
+00:51:13,750 --> 00:51:21,330
+احنا ما ذكرناها مثلا ممكن انك يعني تختاريها و
+
+679
+00:51:21,330 --> 00:51:29,250
+تبحثي عنها تكتبيلي عن فكرة عملها و استخدامها و
+
+680
+00:51:29,250 --> 00:51:36,850
+مبدأ عملها بشكل يعني مبسط تماميعني بدي واجب هيك من
+
+681
+00:51:36,850 --> 00:51:41,230
+صفحة لصفحة و نص عن جهاز يستخدم في ال medical
+
+682
+00:51:41,230 --> 00:51:44,330
+laboratories سواء من اللي ذكرناهم في المساق أو
+
+683
+00:51:44,330 --> 00:51:49,290
+سواء إذا إنتي واجهتي أثناء بحتك جهاز ما اتطرقناله
+
+684
+00:51:49,290 --> 00:51:53,350
+ممكن أنه أيضا كمان يتغطي من خلال هذا ال report أو
+
+685
+00:51:53,350 --> 00:51:59,770
+هذا ال homework بتمنعكم التوفيق يعطيكم ألف عافية و
+
+686
+00:51:59,770 --> 00:52:00,910
+هى ان شاء الله موفقين
+
diff --git a/PL9fwy3NUQKwY9jl8xw-bWkVZkWcGGyspd/RK30qovrvdg_raw.json b/PL9fwy3NUQKwY9jl8xw-bWkVZkWcGGyspd/RK30qovrvdg_raw.json
new file mode 100644
index 0000000000000000000000000000000000000000..7b702985dcaf1a3ad45c8f010a342e9b5bb08f02
--- /dev/null
+++ b/PL9fwy3NUQKwY9jl8xw-bWkVZkWcGGyspd/RK30qovrvdg_raw.json
@@ -0,0 +1 @@
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أثناء الاستخدام أستخدمه بشكل صحيح correct use و أعتني فيه فطبعا أول حاجة لازم قبل البدء بتشغيل أي جهاز أو باستخدام أي جهاز هذا يعني ينطبق على الكل", "tokens": [2407, 21292, 33753, 26108, 14739, 3224, 41850, 37495, 22653, 5551, 2655, 3555, 3615, 16712, 3615, 20292, 2304, 9307, 5551, 12984, 1863, 16606, 42963, 14851, 9778, 3215, 10943, 5551, 14851, 9778, 40448, 3224, 4724, 8592, 28820, 20328, 5016, 1829, 5016, 3006, 764, 4032, 5551, 34268, 22653, 8978, 3224, 6156, 9566, 3555, 3615, 995, 5551, 12610, 11331, 26108, 3660, 5296, 31377, 2304, 12174, 36150, 29739, 3215, 38207, 39894, 8592, 17082, 26895, 36632, 10874, 3224, 31377, 34051, 4724, 995, 14851, 9778, 3215, 10943, 36632, 10874, 3224, 31377, 23758, 37495, 22653, 7251, 1863, 9566, 3555, 4587, 15844, 2423, 28820], "avg_logprob": -0.10017903327631454, "compression_ratio": 1.6894736842105262, "no_speech_prob": 1.6093254089355469e-06, "words": [{"start": 431.41, "end": 432.17, "word": "وبيحتاج", "probability": 0.9549560546875}, {"start": 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635.42, "end": 635.46, "word": " ..", "probability": 0.923828125}, {"start": 635.46, "end": 636.44, "word": " المراد", "probability": 0.7102864583333334}, {"start": 636.44, "end": 637.02, "word": " وزنها", "probability": 0.9801025390625}, {"start": 637.02, "end": 638.48, "word": " مباشرة", "probability": 0.9903564453125}, {"start": 638.48, "end": 639.02, "word": " مهما", "probability": 0.8269856770833334}, {"start": 639.02, "end": 639.34, "word": " كان", "probability": 0.99755859375}, {"start": 639.34, "end": 639.72, "word": " نوع", "probability": 0.969482421875}, {"start": 639.72, "end": 640.06, "word": " هذه", "probability": 0.52490234375}, {"start": 640.06, "end": 640.52, "word": " المادة", "probability": 0.9954427083333334}, {"start": 640.52, "end": 640.88, "word": " ممنوع", "probability": 0.8478190104166666}, {"start": 640.88, "end": 641.04, "word": " أنا", "probability": 0.55908203125}, {"start": 641.04, "end": 641.48, "word": " أحطها", "probability": 0.972412109375}, {"start": 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"balance, centrifuge, spectrohotometer او حتى اي جزء اخر احنا ما ذكرناها مثلا ممكن انك يعني تختاريها و تبحثي عنها تكتبيلي عن فكرة عملها و استخدامها و مبدأ عملها بشكل يعني مبسط تمام", "tokens": [29215, 11, 44828, 7181, 11, 6177, 340, 12194, 13606, 1975, 2407, 11331, 49975, 1975, 1829, 10874, 11622, 38207, 1975, 34740, 1975, 5016, 8315, 19446, 29910, 37983, 8315, 11296, 50113, 15040, 3714, 43020, 16472, 4117, 37495, 22653, 6055, 46456, 9640, 1829, 11296, 4032, 6055, 49628, 12984, 1829, 18871, 11296, 6055, 4117, 2655, 21292, 20292, 18871, 6156, 4117, 25720, 6225, 42213, 11296, 4032, 44713, 9778, 3215, 10943, 11296, 4032, 3714, 44510, 10721, 6225, 42213, 11296, 4724, 8592, 28820, 37495, 22653, 3714, 3555, 3794, 9566, 46811, 10943], "avg_logprob": -0.11167279516949373, "compression_ratio": 1.5449735449735449, "no_speech_prob": 1.2516975402832031e-06, "words": [{"start": 3066.57, "end": 3067.23, "word": "balance,", "probability": 0.94189453125}, {"start": 3068.17, "end": 3069.13, "word": " 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3078.39, "word": " انك", "probability": 0.910400390625}, {"start": 3078.39, "end": 3079.05, "word": " يعني", "probability": 0.81396484375}, {"start": 3079.05, "end": 3080.51, "word": " تختاريها", "probability": 0.98828125}, {"start": 3080.51, "end": 3081.33, "word": " و", "probability": 0.8271484375}, {"start": 3081.33, "end": 3083.57, "word": " تبحثي", "probability": 0.968994140625}, {"start": 3083.57, "end": 3083.99, "word": " عنها", "probability": 0.994873046875}, {"start": 3083.99, "end": 3085.69, "word": " تكتبيلي", "probability": 0.8978515625}, {"start": 3085.69, "end": 3085.87, "word": " عن", "probability": 0.9951171875}, {"start": 3085.87, "end": 3086.17, "word": " فكرة", "probability": 0.98095703125}, {"start": 3086.17, "end": 3086.79, "word": " عملها", "probability": 0.98681640625}, {"start": 3086.79, "end": 3087.17, "word": " و", "probability": 0.87548828125}, {"start": 3087.17, "end": 3088.23, "word": " استخدامها", "probability": 0.966796875}, {"start": 3088.23, "end": 3089.25, "word": " و", "probability": 0.9970703125}, {"start": 3089.25, "end": 3089.59, "word": " مبدأ", "probability": 0.7683919270833334}, {"start": 3089.59, "end": 3089.97, "word": " عملها", "probability": 0.9954427083333334}, {"start": 3089.97, "end": 3090.73, "word": " بشكل", "probability": 0.9685872395833334}, {"start": 3090.73, "end": 3091.69, "word": " يعني", "probability": 0.968994140625}, {"start": 3091.69, "end": 3093.23, "word": " مبسط", "probability": 0.9967041015625}, {"start": 3093.23, "end": 3094.03, "word": " تمام", "probability": 0.89501953125}], "temperature": 1.0}, {"id": 141, "seek": 312091, "start": 3095.09, "end": 3120.91, "text": "يعني بدي واجب هيك من صفحة لصفحة و نص عن جهاز يستخدم في ال medical laboratories سواء من اللي ذكرناهم في المساق أو سواء إذا إنتي واجهتي أثناء بحتك جهاز ما اتطرقناله ممكن أنه أيضا كمان يتغطي من خلال هذا ال report أو هذا ال homework بتمنعكم التوفيق يعطيكم ألف عافية و هى ان شاء الله موفقين", "tokens": [40228, 22653, 4724, 16254, 4032, 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\ No newline at end of file
diff --git a/PL9fwy3NUQKwY9jl8xw-bWkVZkWcGGyspd/RK30qovrvdg_raw.srt b/PL9fwy3NUQKwY9jl8xw-bWkVZkWcGGyspd/RK30qovrvdg_raw.srt
new file mode 100644
index 0000000000000000000000000000000000000000..de9e68c4e730b7322dae61590d49d2b9784cee36
--- /dev/null
+++ b/PL9fwy3NUQKwY9jl8xw-bWkVZkWcGGyspd/RK30qovrvdg_raw.srt
@@ -0,0 +1,2744 @@
+1
+00:00:06,980 --> 00:00:10,700
+السلام عليكم ورحمة الله وبركاته طالبات العزيزات
+
+2
+00:00:10,700 --> 00:00:14,680
+اليوم ان شاء الله رح نتحدث عن ال essential lab
+
+3
+00:00:14,680 --> 00:00:20,360
+instruments تمام؟ رح نتطرق لبعض أجهزة المختبر و
+
+4
+00:00:20,360 --> 00:00:27,450
+نتعرف عن مبدأ عملها، طريقة عملها و استخدامهابما
+
+5
+00:00:27,450 --> 00:00:34,470
+انه كان المساق كله تعريفي بالمختبر و أدواته و
+
+6
+00:00:34,470 --> 00:00:40,190
+أجهزته و يعني كان في بعض الفحوصات الأساسية اللي
+
+7
+00:00:40,190 --> 00:00:44,650
+احنا اتعلمناها و كنا بنستخدم بعض الأجهزة و اتعرفنا
+
+8
+00:00:44,650 --> 00:00:49,390
+على centrifuge و مبدأ عمل و استخدام و اتعرفنا على
+
+9
+00:00:49,390 --> 00:00:54,570
+spectrophotometer و ال microscope كمان اليوم راح
+
+10
+00:00:54,570 --> 00:00:58,600
+نذكر بعض الأجهزة الأخرىالسلام عليكمزي ال balances
+
+11
+00:00:58,600 --> 00:01:04,880
+طبعا الموازين ال balances هي عبارة عن essential
+
+12
+00:01:04,880 --> 00:01:11,680
+laboratory instruments أجهزة أساسية في المختبر و
+
+13
+00:01:11,680 --> 00:01:15,760
+طبعا هي widely used for weighing of various
+
+14
+00:01:15,760 --> 00:01:20,400
+substances طبعا احنا بنستخدمها لوزن المواد سواء
+
+15
+00:01:20,400 --> 00:01:24,660
+كانت هذه المواد powder, crystals المهم مواضة صلبة
+
+16
+00:01:24,660 --> 00:01:29,720
+يتم وزنها باستخدام ال balancesتمام و طبعا لحتى
+
+17
+00:01:29,720 --> 00:01:35,320
+نحضر reagents و سبغات culture media اللي هي المادة
+
+18
+00:01:35,320 --> 00:01:39,040
+المغذية لتنمية البكتيريا و التعرف عليها و تشخيص
+
+19
+00:01:39,040 --> 00:01:44,500
+الأمراض البكتيرية و حتى الفطريات بيلزمنا اني لحتى
+
+20
+00:01:44,500 --> 00:01:48,600
+احضر وسط ينمو عليه البكتيريا او الفطر ان انا اوزن
+
+21
+00:01:48,600 --> 00:01:54,940
+من هاي المادة المغذية و ادوبها ب ..this solvent
+
+22
+00:01:54,940 --> 00:02:00,280
+تمام اذا ال balances بتستخدم for weighing
+
+23
+00:02:00,280 --> 00:02:05,020
+accurately and precisely طبعا within a needed
+
+24
+00:02:05,020 --> 00:02:10,800
+range طبعا بيكون فيها الميزان range بيتحمل وزن من
+
+25
+00:02:10,800 --> 00:02:14,800
+الى وطبعا انا بستخدمه طبقا لهذا ال range نفس فكرة
+
+26
+00:02:14,800 --> 00:02:17,420
+ال by bit يعني انا بستخدم ال by bit علشان انقل
+
+27
+00:02:17,420 --> 00:02:22,850
+volume وكل by bit بيكون لها range خاص فيهاتمام إذا
+
+28
+00:02:22,850 --> 00:02:29,590
+ال balances هي عبارة عن موازين أو ميزان يستخدم في
+
+29
+00:02:29,590 --> 00:02:33,350
+وزن المواد الصلبة powder أو crystal سواء لو كنت
+
+30
+00:02:33,350 --> 00:02:40,730
+بدي أحضر محليل reagents صبغات buffer محليل ملحية
+
+31
+00:02:40,730 --> 00:02:45,090
+سواء كان بدي أحضر cultural media وطبعا في عندي
+
+32
+00:02:45,090 --> 00:02:49,010
+نوعين من هاي الموازينال rough balances أو ال
+
+33
+00:02:49,010 --> 00:02:52,870
+mechanical balances و ال analytical balances تمام
+
+34
+00:02:52,870 --> 00:02:57,050
+نيجي نتعرف على ال rough balances هي عبارة عن طبعا
+
+35
+00:02:57,050 --> 00:03:03,130
+mechanical يعني يتم الوزن و الضبط يدوي و يحتوي على
+
+36
+00:03:03,130 --> 00:03:06,910
+ال .. هذا الميزان على sliding scaleهنا بيكون في
+
+37
+00:03:06,910 --> 00:03:10,630
+تدريج وبيكون في عندي قطع بتنزلق على هذا التدريج
+
+38
+00:03:10,630 --> 00:03:15,690
+بتتحرك يمينا ويسارا تمام؟ مجرد ما انا اوضع المعدة
+
+39
+00:03:15,690 --> 00:03:20,930
+المراد وزنها ببدأ احرك هذه القطع اللي بتنزلق على
+
+40
+00:03:20,930 --> 00:03:26,990
+ال scale على التدريج لحد ما المؤشر هنا يصل لمنتصف
+
+41
+00:03:26,990 --> 00:03:31,390
+يعني انه خلاص صار في balanceتمام صار في اتزان في
+
+42
+00:03:31,390 --> 00:03:36,890
+ال .. هذا الميزان و بقى اخد .. بعرف اللي يعني لحد
+
+43
+00:03:36,890 --> 00:03:39,970
+ما وين وصلت القطعة اللي انزلقت بقى باخد القراءة
+
+44
+00:03:39,970 --> 00:03:43,410
+على التدريج و بعرف انه هذا الوزن طبعا مثل ما انتوا
+
+45
+00:03:43,410 --> 00:03:47,270
+شايفين هذا ال rough balancesمش كتير sensitive يعني
+
+46
+00:03:47,270 --> 00:03:50,730
+مابيقدر يفرقلي ما بين ال .. ال .. يعني الفروق
+
+47
+00:03:50,730 --> 00:03:56,710
+البسيطة في ال .. في الأوزان و أيضا يعني بيستخدم ل
+
+48
+00:03:56,710 --> 00:04:00,030
+ال large amount يعني انا لو بدي أوزن كميات أو
+
+49
+00:04:00,030 --> 00:04:04,190
+أوزان عالي أو أوزان كبيرة و لما بدي يكون عندي هاي
+
+50
+00:04:04,190 --> 00:04:10,420
+الأوزان مش مطلوبيعني high degree of accuracy مش ..
+
+51
+00:04:10,420 --> 00:04:16,860
+مش متطلب مني أنه يكون عندى يعني درجة دقة و صحة
+
+52
+00:04:16,860 --> 00:04:20,960
+عالية فممكن أنا أستخدم هذه ال rough balances طبعا
+
+53
+00:04:20,960 --> 00:04:24,560
+هاي ال rough balances ما بتحتاج main electricity
+
+54
+00:04:24,560 --> 00:04:27,160
+ما بتحتاج أن أنا أشبكها بالكهرب أو أن يكون في
+
+55
+00:04:27,160 --> 00:04:34,320
+عندها battery .. بطاريةطبعا حكينا إنها هي less
+
+56
+00:04:34,320 --> 00:04:40,420
+expensive مش غالية وكمان مش sensitive طبعا إلا إنه
+
+57
+00:04:40,420 --> 00:04:45,700
+في بعض الأنواع ممكن يعني يفرق ما بين ال .. ال ..
+
+58
+00:04:45,700 --> 00:04:50,560
+يعني الموازين وممكن يوزن لي لحد point one gram of
+
+59
+00:04:50,560 --> 00:04:55,900
+substanceتمام؟ هذا ال .. ما يتعلق بال mechanical
+
+60
+00:04:55,900 --> 00:04:59,000
+balance ال analytical balance لأ هو دقيق جدا هو
+
+61
+00:04:59,000 --> 00:05:03,420
+اللي اشتغلنا عليه لما كنا نوزن الماء و نعمل
+
+62
+00:05:03,420 --> 00:05:07,960
+calibration لل pipet طبعا هو دقيق جدا و طبعا
+
+63
+00:05:07,960 --> 00:05:13,400
+بيحتاج مما ان هو analytical و electronic لازم انا
+
+64
+00:05:13,400 --> 00:05:18,610
+يكون مشبوك بالكهرباو ممكن يكون او بيشتغل على
+
+65
+00:05:18,610 --> 00:05:22,350
+بطارية need main electricity او battery و برضه
+
+66
+00:05:22,350 --> 00:05:26,270
+كمان ان هو highly sensitive حساس جدا ممكن يفرقلي
+
+67
+00:05:26,270 --> 00:05:31,190
+بين الأوزان بدقة يعني لو point واحد فارق بالوزن
+
+68
+00:05:31,190 --> 00:05:37,010
+ممكن يوضحلي ياه و طبعا high accurate and precise
+
+69
+00:05:37,010 --> 00:05:42,770
+يعني instrument و طبعا بيوزن بمبدأ ال electro ..
+
+70
+00:05:42,770 --> 00:05:46,980
+ال electromagnetic forceطمام؟ يعني أنا مش محتاجة
+
+71
+00:05:46,980 --> 00:05:50,560
+أن أنا أعاير إشي بمقابل الوزن علشان أ .. يعني ما
+
+72
+00:05:50,560 --> 00:05:53,780
+.. مش .. مش محتاجة أحصل على المكيل هاي معدنية و
+
+73
+00:05:53,780 --> 00:05:57,840
+أحطهم و أكيل أو أن أنا أحرك على sliding scale لبن
+
+74
+00:05:57,840 --> 00:06:01,760
+ما يتزن المؤشر إلى نص الميزان زي ما بنعمل بال ..
+
+75
+00:06:01,760 --> 00:06:07,630
+ال .. ال ..ال .. ال rough balances طبعا مش محتاجة
+
+76
+00:06:07,630 --> 00:06:13,310
+لهذا الاشي طبعا ال rough balances ممكن يكون ب one
+
+77
+00:06:13,310 --> 00:06:19,850
+ban يعني إله دفة واحدة أو double ban طبعا دفتانطيب
+
+78
+00:06:19,850 --> 00:06:24,190
+فبالتالي إحنا مش محتاجين إننا يعني إشي أعاير فيه
+
+79
+00:06:24,190 --> 00:06:28,030
+sliding scale أو أوزان معيارية إنما بطريقة ..
+
+80
+00:06:28,030 --> 00:06:33,010
+بمبدأ عملي الـ electromagnetic force ممكن يعني ..
+
+81
+00:06:33,010 --> 00:06:37,510
+يعني كام بي .. يعني ال .. المادة ينشأ عنها
+
+82
+00:06:37,510 --> 00:06:42,650
+magnetic forceبيعطيني وزن لأ لها، تمام؟ طيب
+
+83
+00:06:42,650 --> 00:06:46,710
+بيستخدم لما أنا بدي أوزن أوزان صغيرة جدا و لما
+
+84
+00:06:46,710 --> 00:06:53,110
+بيكون في عندي ال high accuracy يعني مطلوبة، تمام؟
+
+85
+00:06:53,110 --> 00:06:56,910
+when great accuracy is requiredعلى عكس اللي هي ال
+
+86
+00:06:56,910 --> 00:07:01,550
+rough balances طيب أنا كيف بدي استخدم هذا الميزان
+
+87
+00:07:01,550 --> 00:07:06,050
+و كيف انا بدي اعمله care اهتم فيه؟ طبعا ال balance
+
+88
+00:07:06,050 --> 00:07:10,190
+هو عبارة عن جهاز instrument حساس delicate
+
+89
+00:07:10,190 --> 00:07:14,630
+instrumentوبيحتاج أنه أنا يعني أتبع التعليمات
+
+90
+00:07:14,630 --> 00:07:18,810
+أثناء الاستخدام أستخدمه بشكل صحيح correct use و
+
+91
+00:07:18,810 --> 00:07:24,890
+أعتني فيه فطبعا أول حاجة لازم قبل البدء بتشغيل أي
+
+92
+00:07:24,890 --> 00:07:31,170
+جهاز أو باستخدام أي جهاز هذا يعني ينطبق على الكلمن
+
+93
+00:07:31,170 --> 00:07:34,810
+ضمنهم ال balances أنه أنا أقرأ ال manufacturer
+
+94
+00:07:34,810 --> 00:07:39,310
+instruction طبعا بيجي catalog مع كل جهاز من الشركة
+
+95
+00:07:39,310 --> 00:07:43,830
+المصنعة و في تعليمات كيفية الاستخدام و الدبط و
+
+96
+00:07:43,830 --> 00:07:50,410
+المعايرة و كيفية التشغيل و لو كام .. حتى يعني في
+
+97
+00:07:50,410 --> 00:07:53,600
+خلل معين ممكن كيف أتفاداهكل هذه الشغلات بيكون
+
+98
+00:07:53,600 --> 00:07:57,160
+موجودة فيه ال catalog فانا لازم اقرأ هذا ال
+
+99
+00:07:57,160 --> 00:07:59,820
+catalog او ال instruction حتى كمان التركيب طريقة
+
+100
+00:07:59,820 --> 00:08:02,960
+التركيب اذا كان اجا الجهاز عبارة عن قطع و انا بدي
+
+101
+00:08:02,960 --> 00:08:06,100
+اركبهم برضه كمان بيكون موجود في ال manufacturer
+
+102
+00:08:06,100 --> 00:08:10,350
+instruction and catalogalways handle a balance
+
+103
+00:08:10,350 --> 00:08:13,010
+with a career طبعا لازم انا يعني اتعامل مع ال
+
+104
+00:08:13,010 --> 00:08:19,870
+balance و استخدمه باهتمام برعاية جدا و طبعا
+
+105
+00:08:19,870 --> 00:08:23,250
+position the balance on a firm bench away from
+
+106
+00:08:23,250 --> 00:08:28,310
+vibration طبعا انا بحاول ان انا احط الميزان على
+
+107
+00:08:28,310 --> 00:08:33,090
+بنش ثابت بعيد عن الاهتزازات يعني مايكون تحت مروحة
+
+108
+00:08:33,090 --> 00:08:36,370
+او مقابل يعني
+
+109
+00:08:37,830 --> 00:08:42,250
+الـ تكييف مثلا إذا إجا يعني طيار الهواء بيخبط فيه
+
+110
+00:08:42,250 --> 00:08:45,810
+هذا هيأثر و يكون بعيد مثلا عن التلاجة انتوا
+
+111
+00:08:45,810 --> 00:08:51,050
+بتعرفوا مثلا تلاجة، computer، أجهزة فيها hardware
+
+112
+00:08:51,050 --> 00:08:55,070
+و software ممكن إنها أيضا ان تعملي ذبذبات و
+
+113
+00:08:55,070 --> 00:08:59,530
+إهتزازات فهذا رح يأثرأيضا ببعده عن الهواء وضوء
+
+114
+00:08:59,530 --> 00:09:04,310
+الشمس ببعده انه يتعلق لرطوبة او ي .. يصير يعني ..
+
+115
+00:09:04,310 --> 00:09:07,930
+كمان حتى ببعده عن ال tap water حتى ما يجي عليه ميه
+
+116
+00:09:07,930 --> 00:09:12,070
+و يصدي فهذه الشغلات كمان يعني تدخل من ضمن ال care
+
+117
+00:09:12,070 --> 00:09:16,190
+و انه انا كيف احافظ على الجهازbefore starting to
+
+118
+00:09:16,190 --> 00:09:20,010
+weigh zero the balance طبعا قبل استخدام هذا
+
+119
+00:09:20,010 --> 00:09:24,810
+الميزان لازم انا اصفر و اذا كنت بستخدم beam
+
+120
+00:09:24,810 --> 00:09:27,610
+balance لازم اتأكد على ال position لل beam ايش
+
+121
+00:09:27,610 --> 00:09:31,170
+يعني ال beam balance؟ شوفتوا الميزان اللي بيكون
+
+122
+00:09:31,170 --> 00:09:35,370
+على رمز المحكمة او العدالة اللي هو عبارة عن دفتين
+
+123
+00:09:35,370 --> 00:09:41,400
+معلقات بجان Zero فيهم في ..يعني زي حامل بالنص
+
+124
+00:09:41,400 --> 00:09:45,800
+بالمركز طبعا انا لازم اتأكد من انه هدولة ال .. ال
+
+125
+00:09:45,800 --> 00:09:49,660
+.. الحامل اللي بالمركز و الدفتين موضوع يعني ال
+
+126
+00:09:49,660 --> 00:09:53,740
+position تبعهم كتير منيح مضبوط علشان انا استخدمهم
+
+127
+00:09:53,740 --> 00:09:57,240
+تمام؟ هو بيكون ب .. بيكون بحاجة لأوزان معيارية
+
+128
+00:09:57,240 --> 00:10:01,220
+بهذه الحالةطبعا إذا أنا بدي أوزن chemicals على
+
+129
+00:10:01,220 --> 00:10:06,260
+درجة حرارة الغرفة لازم أحط المادة المراد وزنها ب
+
+130
+00:10:06,260 --> 00:10:12,360
+container ب .. cup أو small beaker طبعا علشان ..
+
+131
+00:10:12,360 --> 00:10:15,000
+طبعا أنتوا كنتوا لما توقعت وزنه يعني أكيد لازم
+
+132
+00:10:15,000 --> 00:10:19,180
+تستخدمي cup فاضي، لازم تستخدمي beaker أو ..كنت
+
+133
+00:10:19,180 --> 00:10:25,400
+أحيان تتوزن على ورقة في النهاية أنه أنا طبعا ما ..
+
+134
+00:10:25,400 --> 00:10:28,280
+يعني ما أحط ال .. ال chemical never put the
+
+135
+00:10:28,280 --> 00:10:31,200
+chemical directly on the balance فعلا يعني على دفة
+
+136
+00:10:31,200 --> 00:10:35,460
+ال .. الميزان ما أحط المادة الكيميائية ال .. ال ..
+
+137
+00:10:35,460 --> 00:10:40,880
+المراد وزنها مباشرة مهما كان نوع هذه المادة ممنوع
+
+138
+00:10:40,880 --> 00:10:44,500
+أنا أحطها لإنها ممكن إنها ..تطلف الجهاز when
+
+139
+00:10:44,500 --> 00:10:48,200
+adding or removing a chemical remove the container
+
+140
+00:10:48,200 --> 00:10:52,740
+to avoid spilling and chemical on the balance طبعا
+
+141
+00:10:52,740 --> 00:10:56,300
+لما انا بدي اوزن لازم احط المادة المراد وزنها في
+
+142
+00:10:56,300 --> 00:10:58,840
+هذا ال container و لما بدي ارفع برفع ال container
+
+143
+00:10:58,840 --> 00:11:03,280
+كله علشان في النهاية انا بدي امنع انسكاب اي مادة
+
+144
+00:11:03,280 --> 00:11:06,900
+كيميائية او يعني انها تنكب على الميزان ممكن انها
+
+145
+00:11:06,900 --> 00:11:10,020
+تتسرب الى الداخل تعمل انسدادات او تأثر على الجهاز
+
+146
+00:11:10,020 --> 00:11:16,110
+من جواأو حتى تلوث الجهاز أو تخربه always use
+
+147
+00:11:16,110 --> 00:11:19,590
+forceps to add or remove weight طبعا انا اذا بدي
+
+148
+00:11:19,590 --> 00:11:24,130
+انقل مادة لحتى اوزنها او يعني اشي لازم انا استخدم
+
+149
+00:11:24,130 --> 00:11:27,050
+ال forceps بالملقط او حتى استخدم ال spatula اللي
+
+150
+00:11:27,050 --> 00:11:30,530
+هي المعلقة المعدنية الصغيرة المخصصة لنقل المواد
+
+151
+00:11:30,530 --> 00:11:34,980
+المراد وزنهاطيب protect the weight from dust إن
+
+152
+00:11:34,980 --> 00:11:40,940
+أنا أحاول أحافظ على الجهاز من الغبار والرطوبة وإنه
+
+153
+00:11:40,940 --> 00:11:45,980
+ما ينمو عليه الفطريات
+
+154
+00:11:45,980 --> 00:11:51,200
+ومثلا بغطي الجهاز إذا كان إله cover طبعا و الأوزان
+
+155
+00:11:51,200 --> 00:11:54,800
+اللي بوزنها بحاول إن أنا كمانيعني اللى مابدي
+
+156
+00:11:54,800 --> 00:11:57,620
+أوزنها أستخدمها أغطيها إذا مابدي أستخدمها مباشرة
+
+157
+00:11:57,620 --> 00:12:01,540
+لحتى برضه كمان أحافظ عليها ممكن أي غبراء، رطوبة أو
+
+158
+00:12:01,540 --> 00:12:06,480
+حتى يعني تعفن فيها يأثر على وزنها وجودتها ويأثر
+
+159
+00:12:06,480 --> 00:12:08,540
+على المحليل اللى أنا بدي أحضرها باستخدام هذه
+
+160
+00:12:08,540 --> 00:12:11,920
+الأوزانuse a small brush to remove any chemical
+
+161
+00:12:11,920 --> 00:12:16,580
+فهي بيكون عندي فرشاية مخصصة ل .. لكنز البان
+
+162
+00:12:16,580 --> 00:12:21,600
+للميزان و دفة الميزان لحتى مايضل عالق عليه أي
+
+163
+00:12:21,600 --> 00:12:26,060
+أجزاء من المادة المراد وزنها فبالتالي هيك أنا بضل
+
+164
+00:12:26,060 --> 00:12:30,810
+محافظة على الجهاز ال ovenال ovens اللي هي الأفران
+
+165
+00:12:30,810 --> 00:12:36,750
+أيضا بنستخدم أفران في المختبرات طبعا في عندي hot
+
+166
+00:12:36,750 --> 00:12:42,070
+air oven اللي هو ال .. ال oven اللي بي .. بي طبعا
+
+167
+00:12:42,070 --> 00:12:45,630
+فرن بس مش بيكون في حرارة يعني في نار مولعة أو في
+
+168
+00:12:45,630 --> 00:12:49,830
+لهب أو في إشي لأ هو بي .. يعني بيبدأ .. بيشتغل
+
+169
+00:12:49,830 --> 00:12:53,310
+بمبدأ عمل تدوير الهواء الساخن يعني في هوا ساخن
+
+170
+00:12:53,310 --> 00:12:58,760
+بيطلعفي الجهاز بي .. بيتم دورانه في الجهاز فبي ..
+
+171
+00:12:58,760 --> 00:13:02,640
+بيسخن الأشياء اللي .. اللي موجودة في الجهاز طبعا
+
+172
+00:13:02,640 --> 00:13:06,760
+بيستخدم ال oven for drying لتشفيف ال chemicals إذا
+
+173
+00:13:06,760 --> 00:13:11,400
+كان أنا حاسيت إنه المادة ممتصة رطوبة أو يعني مبتلة
+
+174
+00:13:11,400 --> 00:13:17,400
+أو حتى أنا بدي يعني أركزها مادة مدعبة و بدي أركزها
+
+175
+00:13:17,400 --> 00:13:21,740
+ممكن أستخدم ال .. ال oven للتشفيف و كده أيضا برضه
+
+176
+00:13:21,740 --> 00:13:27,790
+كمان إذا كان في عنديمواد زجاجية أنا غسلتها و
+
+177
+00:13:27,790 --> 00:13:31,430
+نضفتها و بدي أجففها لإنه بدي أستخدمها يعني من شروط
+
+178
+00:13:31,430 --> 00:13:35,210
+الاستخدام إنها تكون جافة تماما إذا كنت بحضر محلول
+
+179
+00:13:35,210 --> 00:13:38,650
+و لازم تكون جافة تماما و التركيز و ال .. و ال ..
+
+180
+00:13:38,650 --> 00:13:41,250
+هادي بتاعي بيعنيلي إنه يكون دقيق فبالتالي ال
+
+181
+00:13:41,250 --> 00:13:44,610
+glassware لازم تكون جافة و مافيها بقايا ماء أيضا
+
+182
+00:13:44,610 --> 00:13:49,650
+هادي لازم تجفف بال ovenأيضا يستخدم ال oven ل ال
+
+183
+00:13:49,650 --> 00:13:52,310
+sterilization إذا مش بس ل ال drying ل ال
+
+184
+00:13:52,310 --> 00:13:56,630
+sterilization كمان ل تعقيم ال glassware و ال metal
+
+185
+00:13:56,630 --> 00:13:59,750
+instrument الأدوات المعدنية و الأدوات الزجاجية
+
+186
+00:13:59,750 --> 00:14:06,230
+طبعا هو مكون من double wall من طبعا جدرانه عبارة
+
+187
+00:14:06,230 --> 00:14:14,730
+عن طبقة مزدوجة من النحاس أو المعدنطبعا و بيسخن by
+
+188
+00:14:14,730 --> 00:14:18,970
+circulation of hot air حكينا انه من خلال تدوير
+
+189
+00:14:18,970 --> 00:14:25,290
+الهواء الساخن طبعا بيكون فيها gas burner في لهب في
+
+190
+00:14:25,290 --> 00:14:29,590
+منطقة ما في الجهاز و بتسخن الهواء و الهواء طبعا
+
+191
+00:14:29,590 --> 00:14:34,630
+بيتنضخه داخل الجهاز و تدويره طبعا في عندي
+
+192
+00:14:34,630 --> 00:14:37,370
+thermometer بيقيص على .. على .. بيكون موجود على
+
+193
+00:14:37,370 --> 00:14:43,880
+جدار او على ..باب الجهاز في ال .. في الأعلى بيكون
+
+194
+00:14:43,880 --> 00:14:47,620
+a thermometer بيقيص درجة حرارة الجهاز و thermostat
+
+195
+00:14:47,620 --> 00:14:51,800
+بيعمل regulation لدرجة الحرارة طبعا؟ اذا في عندي
+
+196
+00:14:51,800 --> 00:14:56,160
+قياس لدرجة الحرارة و ضبط لهذه الدرجة طبعا انا ب ..
+
+197
+00:14:56,160 --> 00:15:00,100
+بعمله on في عندي in, on و off و في عندي هنا scale
+
+198
+00:15:00,100 --> 00:15:02,600
+بي .. انا بختار درجة الحرارة اللي انا بدي أجفف
+
+199
+00:15:02,600 --> 00:15:04,020
+عليها أو بدي أعقم عليها
+
+200
+00:15:07,160 --> 00:15:11,200
+ال water bath ال water bath هو عبارة عن a
+
+201
+00:15:11,200 --> 00:15:15,180
+instrument where water is heated and the seat
+
+202
+00:15:15,180 --> 00:15:18,480
+temperature is maintained at constant level طبعا
+
+203
+00:15:18,480 --> 00:15:23,460
+يعني أنا هنا بستخدم باستخدام درجة حرارة ثابتة بسخن
+
+204
+00:15:23,460 --> 00:15:29,170
+الماءو في داخل هذا الجهاز و بستخدمه كوسط مائي أو
+
+205
+00:15:29,170 --> 00:15:33,450
+حمام مائي طبعا ليش؟ to incubate liquid substance
+
+206
+00:15:33,450 --> 00:15:36,530
+طبعا كان في عندنا في تفاعلات ال biochemistry
+
+207
+00:15:36,530 --> 00:15:42,450
+incubate the tube على درجة حرارة كذا، تمام؟ فكان
+
+208
+00:15:42,450 --> 00:15:46,290
+لازم انك انت بعد ما تجري التفاعلتحط A على B
+
+209
+00:15:46,290 --> 00:15:50,170
+Reagent A على .. او مادة A على مادة B علشان تطلع
+
+210
+00:15:50,170 --> 00:15:53,950
+المادة C اللي هي ال .. ال .. الناتج كان لازم انك
+
+211
+00:15:53,950 --> 00:15:57,270
+انت تعملي incubation على درجة حرارة معينة طبعا
+
+212
+00:15:57,270 --> 00:16:01,370
+باستخدام ال water بقى طبعا ان هو بوفرلي درجة حرارة
+
+213
+00:16:01,370 --> 00:16:05,450
+معينة لأ التفاعل عشان انا اعمل incubation زي تحضين
+
+214
+00:16:05,450 --> 00:16:09,310
+للانابيب على درجة حرارة معينة لحتى يتم التفاعل
+
+215
+00:16:09,310 --> 00:16:16,680
+تمام؟طيب when only a few samples in a tube require
+
+216
+00:16:16,680 --> 00:16:19,800
+incubation it's more convenient and less expensive
+
+217
+00:16:19,800 --> 00:16:23,680
+to use a dry hot heat block طبعا لما بيكون عندى
+
+218
+00:16:23,680 --> 00:16:26,720
+كمية العينة قليلة او المحاليه القليله و الانابيب
+
+219
+00:16:26,720 --> 00:16:29,760
+الصغيرة زى الabendrof بتعرفوا الabendrof التيوب
+
+220
+00:16:29,760 --> 00:16:32,720
+اللى هى الانابيب اللى اعطيتكن فيها عينة ال blood
+
+221
+00:16:32,720 --> 00:16:38,560
+فى المتحان العملي انابيب صغيرة جدا طبعا لو انا ..
+
+222
+00:16:38,710 --> 00:16:42,570
+بشتغل فيها يعني بشتغل في كميات قليلة فلازم أنا
+
+223
+00:16:42,570 --> 00:16:45,590
+أستخدم بدل ما أستخدم ال water bath بستخدم ال heat
+
+224
+00:16:45,590 --> 00:16:50,170
+block تمام؟ يعني هو عبارة عن block معدني بيسخن عن
+
+225
+00:16:50,170 --> 00:16:53,210
+درجة حرارة معينة و بيظل ثابت درجة الحرارة و برضه
+
+226
+00:16:53,210 --> 00:16:55,630
+نفس الاشي بيعملي incubation لإن أنا بيبقى على درجة
+
+227
+00:16:55,630 --> 00:16:59,870
+الحرارة اللي بديها يعني أنا بختارها هذه الدرجةطيب
+
+228
+00:16:59,870 --> 00:17:03,690
+فبالتالي هو عبارة عن ال dry bath اذا هو مش water
+
+229
+00:17:03,690 --> 00:17:06,470
+bath لأ dry bath لأنه زي ما انتوا شايفين مش water
+
+230
+00:17:06,470 --> 00:17:09,970
+في الموضوع و هي block معدني بيسخن و بيبرد حسب درجة
+
+231
+00:17:09,970 --> 00:17:14,070
+الحرارة اللي انا بدي اياها تمام ال chemical test
+
+232
+00:17:14,070 --> 00:17:17,850
+التفاعلات الكيميائية react test at specific
+
+233
+00:17:17,850 --> 00:17:21,730
+temperature طبعا كل تفاعل كيميائي له درجة حرارة
+
+234
+00:17:21,730 --> 00:17:26,470
+خاصة بيشتغل عليها و بيكون افضل reaction او افضل
+
+235
+00:17:26,470 --> 00:17:31,670
+نتيجة على هاي درجة الحرارةmany tests react at room
+
+236
+00:17:31,670 --> 00:17:35,990
+temperature في تفاعلات احنا كنا بيمكن نعمل لهم
+
+237
+00:17:35,990 --> 00:17:40,210
+incubation على درجة حرارة الغرفة من 18 ل 22 مثلا
+
+238
+00:17:40,210 --> 00:17:46,310
+زي ال .. فحص ال homoglobin و فحص ال glucose كنا
+
+239
+00:17:46,310 --> 00:17:50,940
+نستنى 10 دقائق في درجة حرارة الغرفةطيب و لو أنا
+
+240
+00:17:50,940 --> 00:17:55,200
+بدي .. ممكن بقى أحيانا التفاعلات بتكون بتحتاج درجة
+
+241
+00:17:55,200 --> 00:17:59,340
+حرارة 37 أيضا ال homoglobin و ال glucose كان ممكن
+
+242
+00:17:59,340 --> 00:18:01,940
+أن أنا أحطهم على .. في ال water بس على درجة حرارة
+
+243
+00:18:01,940 --> 00:18:08,220
+37 و أستنى خمس دقائق مش عشرة طبعا فإذا ال .. ال
+
+244
+00:18:08,220 --> 00:18:12,220
+incubation time بيختلف من درجة حرارة إلى أخرى و
+
+245
+00:18:12,220 --> 00:18:16,260
+أيضا يعني كل تفاعل له ال bestincubation
+
+246
+00:18:16,260 --> 00:18:21,360
+temperature تمام؟ فأنا بضبط ال .. ال water bath
+
+247
+00:18:21,360 --> 00:18:25,340
+على حسب ال .. الدرجة الحرارة الأفضل للتفاعل اللي
+
+248
+00:18:25,340 --> 00:18:31,540
+أنا عم بشتغلهuse and care of water bath كيف أنا
+
+249
+00:18:31,540 --> 00:18:35,700
+أستخدم جهاز ال water bath و كيف أهتم فيه طبعا زي
+
+250
+00:18:35,700 --> 00:18:38,860
+ما انتوا شايفين read manufacturing instruction هذي
+
+251
+00:18:38,860 --> 00:18:43,140
+هتشوفوها في كل الأجهزة كأول بند ان انا لازم أقرأ
+
+252
+00:18:43,140 --> 00:18:46,420
+تعليمات الجهة المصنعة و ال catalog الخاص بالجهاز
+
+253
+00:18:46,420 --> 00:18:50,120
+لحتى أفهم كل شي بيتعلق فيه تركيبه تشغيله
+
+254
+00:18:50,120 --> 00:18:53,820
+calibration لإله إذا بيحتاج calibration و نصائح
+
+255
+00:18:53,820 --> 00:18:58,440
+أخرىFill the bath and maintain its level with
+
+256
+00:18:58,440 --> 00:19:02,260
+distilled water if unavailable with boiled water
+
+257
+00:19:02,260 --> 00:19:07,320
+طبعا أنا بدي أملأ هذا الجهاز بالـ distilled water،
+
+258
+00:19:07,320 --> 00:19:10,300
+يفضل distilled water عشان ما لو انا بدي استخدم ال
+
+259
+00:19:10,300 --> 00:19:15,690
+tap water، ال tap water فيها أملحو ال .. ممكن ترسب
+
+260
+00:19:15,690 --> 00:19:20,750
+على جدران ال water bath و تؤدي إلى تلفة فبالتالي
+
+261
+00:19:20,750 --> 00:19:23,150
+يفضل إن أنا أستخدم distilled water إذا ما ..
+
+262
+00:19:23,150 --> 00:19:25,750
+ماعندي distilled water متوفر ممكن أستخدم الماء
+
+263
+00:19:25,750 --> 00:19:31,000
+المغلي اللي هو ال boiled waterطيب و طبعا ليش حكينا
+
+264
+00:19:31,000 --> 00:19:34,920
+this is necessary مضروري to minimize salt
+
+265
+00:19:34,920 --> 00:19:40,100
+deposition on the heater عشان ما يقلل ترسيب الأملح
+
+266
+00:19:40,100 --> 00:19:44,680
+في الجهاز to minimize the growth of microorganism
+
+267
+00:19:44,680 --> 00:19:48,940
+طبعا حتى الميه ما تعفن و ما تخضرن و مايصير فيها زي
+
+268
+00:19:48,940 --> 00:19:54,520
+الخضار عفن او زي هيك زي ال ..هي التحالي بيكون فيها
+
+269
+00:19:54,520 --> 00:20:00,040
+لازم انا اضيف مادة معقمة اللي لها disinfectant و
+
+270
+00:20:00,040 --> 00:20:04,220
+bactericidal agent يعني ماء عامل قاتل للبكتيريا
+
+271
+00:20:04,220 --> 00:20:08,100
+اللي هو ال mercellate تمام؟ بضيف للماء اللي انا
+
+272
+00:20:08,100 --> 00:20:12,660
+ضفته في ال water bath ال mercellate طبعا لكل مثلا
+
+273
+00:20:12,660 --> 00:20:18,420
+الف milli يعني لكل one liter مثلا من الماء اللي
+
+274
+00:20:18,420 --> 00:20:27,010
+انا ضفته بحط one ml لكل الف mlتمام بحط one ml من
+
+275
+00:20:27,010 --> 00:20:32,030
+ال mer cellulite يعني ال dilution واحد لأقل before
+
+276
+00:20:32,030 --> 00:20:35,690
+incubating samples ا check the temperature جاب
+
+277
+00:20:35,690 --> 00:20:40,250
+اللي اعمل incubation للعينات لازم اعمل check على
+
+278
+00:20:40,250 --> 00:20:44,330
+ال temperature اشيك عليها و إذا كان محتاجة ان انا
+
+279
+00:20:44,330 --> 00:20:51,080
+مثلايعني أطبطها على درجة حرارة أعلى أو أقل و أيضا
+
+280
+00:20:51,080 --> 00:20:53,560
+ممكن أنا أتأكد من درجة الحرارة باستخدام
+
+281
+00:20:53,560 --> 00:20:59,040
+thermometer طبعا لازم يكون المستوى الماء ال level
+
+282
+00:20:59,040 --> 00:21:02,440
+of water is above the level of water whatever is
+
+283
+00:21:02,440 --> 00:21:06,800
+being incubated يعني معنى هذه الجملة أنه لازم يكون
+
+284
+00:21:06,800 --> 00:21:11,800
+مستوى الماء يغمر الأنابيبالمراد مثلا ال incubation
+
+285
+00:21:11,800 --> 00:21:15,520
+لإلها يعني إذا كان أنا بستخدم أنابيب كبيرة و
+
+286
+00:21:15,520 --> 00:21:18,380
+المحلول اللي فيها عالي بدي أزود نسبة الماء عشان
+
+287
+00:21:18,380 --> 00:21:21,640
+يغمرها عشان يعمل إلها incubation ما بنفعل أنا ..
+
+288
+00:21:21,640 --> 00:21:25,860
+يعني الماء اللي .. اللي في ال incubator أو في ال
+
+289
+00:21:25,860 --> 00:21:29,380
+water bath .. الماء اللي في ال water bath ما بنفع
+
+290
+00:21:29,380 --> 00:21:33,380
+إنه يكون يدوب ملامس ال tube أو مش ملامسها، هي أنا
+
+291
+00:21:33,380 --> 00:21:37,610
+ما بكون عم بعمل incubation، تمام؟raise the
+
+292
+00:21:37,610 --> 00:21:42,750
+temperature to تسعين درجة or higher at once a week
+
+293
+00:21:42,750 --> 00:21:45,690
+for half an hour for the purpose of
+
+294
+00:21:45,690 --> 00:21:49,630
+decontamination بحكيلك مثلا مرة في الإسبوع لمدة
+
+295
+00:21:49,630 --> 00:21:54,230
+مثلا نص ساعة ارفعي درجة الحرارة لتسعين و نزليها
+
+296
+00:21:54,230 --> 00:21:57,610
+تاني او مثلا شغل الجهاز ل .. اضبطيه على تسعيني
+
+297
+00:21:57,610 --> 00:22:01,570
+يسخن الميه و يرجع .. و ارجع يطفي يخليه يبرد تاني
+
+298
+00:22:01,920 --> 00:22:05,780
+ليش؟ هذه درجة الحرارة العالية بتقتل البكتيريا يعني
+
+299
+00:22:05,780 --> 00:22:11,240
+in addition to myrciulate بالإضافة لmyrciulate use
+
+300
+00:22:11,240 --> 00:22:15,160
+the lid to prevent loss of heat from the bath لازم
+
+301
+00:22:15,160 --> 00:22:20,880
+أنا خلال ال .. ال .. ال .. يعني ال incubation time
+
+302
+00:22:20,880 --> 00:22:25,200
+أكون عم بغطي جهاز ال water bath حتى يعني اتضل درجة
+
+303
+00:22:25,200 --> 00:22:29,050
+الحرارة منتظمةما .. ما يبرد الجهاز و يضل يرجع يسخن
+
+304
+00:22:29,050 --> 00:22:34,190
+تاني يعني بضمن ثباتية درجة الحرارة و أيضا حتى ما
+
+305
+00:22:34,190 --> 00:22:37,590
+يتبخر الماء اللي موجود فيها و to minimize
+
+306
+00:22:37,590 --> 00:22:40,550
+particles from entering the water يعني حتى ما
+
+307
+00:22:40,550 --> 00:22:45,870
+يضايق غبار او مواد او اشي ي .. ي .. يعني يقع في
+
+308
+00:22:45,870 --> 00:22:50,550
+الماء و يلوثهwhen removing the lid after
+
+309
+00:22:50,550 --> 00:22:54,570
+incubation take care to avoid any water entering
+
+310
+00:22:54,570 --> 00:23:01,280
+uncapped tubes طبعا لما أنا ب .. بغطي الجهازو
+
+311
+00:23:01,280 --> 00:23:06,180
+برفعه لازم اتأكد انه ال .. القنابيب ما بيفوت فيها
+
+312
+00:23:06,180 --> 00:23:09,060
+البخار الماء اللى بيتساعد و بيتكثف على غطاية
+
+313
+00:23:09,060 --> 00:23:12,680
+الجهاز مايفوت داخل القنابيب اذا لازم انا اكون عم
+
+314
+00:23:12,680 --> 00:23:16,900
+بسكر القنابيب باستخدام الكعب الخاص فيها او
+
+315
+00:23:16,900 --> 00:23:21,120
+باستخدام cotton يعني المهم انه اضمن ان القنابيب
+
+316
+00:23:21,120 --> 00:23:25,960
+مايفوت لمحتوها ماء هيك هيأثر على التركيز اللى عم
+
+317
+00:23:25,960 --> 00:23:29,970
+بشتغل فيها و ايضاإذا أنا بدأ أقرأ شدة لون أو تركيز
+
+318
+00:23:29,970 --> 00:23:35,050
+أو إشي فممكن يأثر على النتيجة clean the water bath
+
+319
+00:23:35,050 --> 00:23:38,690
+regularly طبعا لازم أنا أنظفه باستمرار we take
+
+320
+00:23:38,690 --> 00:23:41,750
+care not to damage the heating unit طبعا بيكون في
+
+321
+00:23:41,750 --> 00:23:50,340
+عندي زي قداء بتسخن زي هيك زيسخان أو زي مادة ..
+
+322
+00:23:50,340 --> 00:23:54,720
+يعني جزء معدني هو اللي بيسخن و بيسخل الميه، تمام؟
+
+323
+00:23:54,720 --> 00:23:59,640
+فهذا الجزء طبعا مش لازم انا احاول .. يعني احاول
+
+324
+00:23:59,640 --> 00:24:04,780
+اتعامل معاه برفق و مش لازم انا اكسره او اطلفه
+
+325
+00:24:05,800 --> 00:24:09,100
+unplug the bath from the wall socket when not
+
+326
+00:24:09,100 --> 00:24:13,380
+using it طبعا مجرد هذا الاشي بيطبق مش بس على ال
+
+327
+00:24:13,380 --> 00:24:18,020
+water bath على كل الأجهزة اللي عم نستخدمها لازم
+
+328
+00:24:18,020 --> 00:24:22,560
+انا مجرد ما اخلص يعني افك الكبش من الحيط يعني من
+
+329
+00:24:22,560 --> 00:24:27,620
+ال .. من ال .. ال .. ال fish نفسها افكها يعني مش
+
+330
+00:24:27,620 --> 00:24:33,400
+لازم اخليه موصل بالكهربا هذا لإنه لحتة أقل منخطر
+
+331
+00:24:33,400 --> 00:24:37,660
+ال .. ال .. الكهربا اللي ممكن يكون موجود في البيئة
+
+332
+00:24:37,660 --> 00:24:41,540
+اللي أنا عم بشتغل فيها every three to six months I
+
+333
+00:24:41,540 --> 00:24:44,640
+check the bath for correction طبعا يعني كل تلت لست
+
+334
+00:24:44,640 --> 00:24:48,480
+شهور بأتأكد من صحة درجة الحرارة اللي بيعطينيها هذا
+
+335
+00:24:48,480 --> 00:24:54,720
+الجهاززي أني مثلا أحط درجة حرارة بشكل random مثلا
+
+336
+00:24:54,720 --> 00:24:59,220
+أربعين درجة أخليه يسخن و يصل لدرجة الأربعين و يثبت
+
+337
+00:24:59,220 --> 00:25:03,200
+عليها بجيب thermometer و بخليه يقيس يشوف لإن ..
+
+338
+00:25:03,200 --> 00:25:06,100
+أتأكد أنه والله ال thermometer بيعطيني أربعين زي
+
+339
+00:25:06,100 --> 00:25:10,140
+ما الجهاز بيعطيني أربعين ولا لأ فهذا يعني بتأكد من
+
+340
+00:25:10,140 --> 00:25:16,760
+صحة درجات الحرارة اللي بيصل لها الجهازIf you are
+
+341
+00:25:16,760 --> 00:25:21,020
+using a boiling water bath and oven, be sure you
+
+342
+00:25:21,020 --> 00:25:25,420
+use heat resistant glass or plasticware طبعا إذا
+
+343
+00:25:25,420 --> 00:25:28,800
+أنا كنت مستخدم سواء كان oven أو حتى water bath
+
+344
+00:25:28,800 --> 00:25:34,160
+ويوصل لدرجة حرارة الغليان لازم ال glass اللي أنا
+
+345
+00:25:34,160 --> 00:25:38,560
+بيه ب اللي بستخدمها أو حتى سواء كانت plastic لازم
+
+346
+00:25:38,560 --> 00:25:42,020
+يكون plastic مقوة بيتحمل درجات حرارة عالية و أيضا
+
+347
+00:25:42,020 --> 00:25:45,680
+نفس الاشي لازم يكون heat resistant glassيعني مثلا
+
+348
+00:25:45,680 --> 00:25:49,800
+يكون ال .. الانابيب الزجاجية مصنوعة من الزجاج ال
+
+349
+00:25:49,800 --> 00:25:54,280
+birex اللي هي أصلا ال .. فعليا هي هذه المادة اللي
+
+350
+00:25:54,280 --> 00:25:58,140
+احنا بنستخدمها في الانابيب الزجاجية اللي بتشتغلوا
+
+351
+00:25:58,140 --> 00:26:03,020
+فيها و في ال flaskات و في ال .. السلندرات و كل إشي
+
+352
+00:26:03,020 --> 00:26:06,020
+زجاجي عندنا من ال birex يعني هو heat resistant
+
+353
+00:26:11,570 --> 00:26:18,730
+طبعا أثناء إدخال وخروج أي أدوات وانابيب من ال
+
+354
+00:26:18,730 --> 00:26:21,110
+incubator إذا أنا عم بشتغل على درجة حرارة عالية
+
+355
+00:26:21,110 --> 00:26:25,590
+لازم أكون مستخدمة كفات خاصة لحتى ما أقدي نفسي أو
+
+356
+00:26:25,590 --> 00:26:31,620
+أحرق نفسيو incubator اللي هو الحاضنة أو الجهاء
+
+357
+00:26:31,620 --> 00:26:35,840
+الأعلى الحاضن أو الحاضن ال incubator طبعا بيعطيني
+
+358
+00:26:35,840 --> 00:26:40,600
+incubation و طبعا على control temperature على درجة
+
+359
+00:26:40,600 --> 00:26:45,320
+حرارة مضبوطةولكن مش لتفاعل هو لأ لل
+
+360
+00:26:45,320 --> 00:26:49,200
+bacteriological culture لشغل مزرعة البكتيريا لما
+
+361
+00:26:49,200 --> 00:26:55,320
+انا بدي اعزل بكتيريا او فطر او يعني ميكروب من عينة
+
+362
+00:26:55,320 --> 00:27:03,140
+مريض طبعا لازم انا احط العينة اضيف العينة تبعته
+
+363
+00:27:03,140 --> 00:27:08,040
+على مادة مغذية تنعش وتثري نمو الميكروب
+
+364
+00:27:11,590 --> 00:27:17,270
+أيضا يعني .. يعني أعمل لها incubation لحتى تنمو
+
+365
+00:27:17,270 --> 00:27:22,610
+على درجة حرارة مثلا 37 ل ال blood transfusion لحفظ
+
+366
+00:27:22,610 --> 00:27:27,790
+وحدات الدم لبعض التفاعلات اللي في قسم السيرولوجي
+
+367
+00:27:27,790 --> 00:27:31,230
+اللي هو الأمصال لقسم ال hematology اللي هو علم
+
+368
+00:27:31,230 --> 00:27:35,310
+الدم لقسم ال chemistryيعني أحيانا مثلا بدي
+
+369
+00:27:35,310 --> 00:27:40,230
+incubation سريع على درجة حرارة 37 بدل ما أستنى لسه
+
+370
+00:27:40,230 --> 00:27:45,110
+بدي أسخن water bath و أصل لدرجة حرارة 37 ال
+
+371
+00:27:45,110 --> 00:27:47,770
+incubator إذا موجود عندكوا already غالبا بيكون
+
+372
+00:27:47,770 --> 00:27:50,810
+مضبوط على درجة حرارة 37 اللي هي درجة حرارة نمو
+
+373
+00:27:50,810 --> 00:27:54,330
+البكتيريا و الفطريات و الميكروبات و في ال .. في ال
+
+374
+00:27:54,330 --> 00:27:58,270
+cultures فبالتالي يعني عادي سريع جدا ممكن أفوت
+
+375
+00:27:58,270 --> 00:28:00,790
+الأنابيب تبعتي أعمللها incubation الوقت اللي بديها
+
+376
+00:28:00,790 --> 00:28:04,500
+و أطلعهافي حالة ان انا لو بشتغل فحوصات chemistry
+
+377
+00:28:04,500 --> 00:28:10,540
+كيميا for bacteriological culture an incubator is
+
+378
+00:28:10,540 --> 00:28:14,980
+required whereas for other tests a dry heat block
+
+379
+00:28:14,980 --> 00:28:22,220
+or water bath may be used حكينا انه ال .. في ال ..
+
+380
+00:28:22,220 --> 00:28:24,920
+في ال bacteriological culture في المزارع البكتيرية
+
+381
+00:28:24,920 --> 00:28:30,130
+احنا بنستخدم ال incubatorلكن في الحصات الأخرى ممكن
+
+382
+00:28:30,130 --> 00:28:35,590
+أستخدم اللي هو ال dry heat block أو ال dry bath
+
+383
+00:28:35,590 --> 00:28:39,310
+incubator أو ال incubator العادي
+
+384
+00:28:41,940 --> 00:28:46,100
+و غالبا بيكون usually على 37 درجة مضبوط و حكينا
+
+385
+00:28:46,100 --> 00:28:49,400
+إنه ممكن أنا أستخدمه لل other test لو أنا بدي درجة
+
+386
+00:28:49,400 --> 00:28:54,360
+حرارة 37 و بشكل سريعي يعني ممكن أستخدمه the
+
+387
+00:28:54,360 --> 00:28:57,500
+appropriate temperature is obtained by means of
+
+388
+00:28:57,500 --> 00:29:01,940
+temperature regulator طبعا بصل لدرجة الحرارة اللي
+
+389
+00:29:01,940 --> 00:29:09,660
+بديها من خلال ال .. regulatorمنظم لدرجة الحرارة
+
+390
+00:29:09,660 --> 00:29:12,720
+بتظهر عندى درجة الحرارة هنا انا لازم اضل اعملها
+
+391
+00:29:12,720 --> 00:29:16,560
+check باستمرار طبعا فى عندى thermostat بنظملى درجة
+
+392
+00:29:16,560 --> 00:29:20,360
+الحرارة بيطلع ليها هنا 37 لازم اضل اشيك عليها
+
+393
+00:29:20,360 --> 00:29:21,100
+باستمرار
+
+394
+00:29:23,590 --> 00:29:27,450
+حتى أتأكد إنها هي 37 يعني إذا زادت درجة الحرارة أو
+
+395
+00:29:27,450 --> 00:29:31,070
+قلت ممكن يصير فيه تأثير على نمو البكتيريا هاي هذه
+
+396
+00:29:31,070 --> 00:29:36,270
+.. هذه أطباق احنا بنستخدمها لزراعة البكتيريا تمام؟
+
+397
+00:29:36,270 --> 00:29:40,680
+أو حتى كمان ممكن لزراعة الفطرياتممكن لل cell
+
+398
+00:29:40,680 --> 00:29:43,980
+culture لزراعة الخلايا سواء كان بشتغل على فيروسات
+
+399
+00:29:43,980 --> 00:29:47,360
+أو بشتغل على خلايا cancer cells مثلا بدي أدرس
+
+400
+00:29:47,360 --> 00:29:50,820
+سلوكها أو بدي أعمل عليها أبحاث خلايا جذعية كلها
+
+401
+00:29:50,820 --> 00:29:54,740
+هذه طبعا لازم تحفظ على درجة حرارة 37 يعني بدي
+
+402
+00:29:54,740 --> 00:29:59,680
+أستخدم incubator طبعا لحتى أعمله use و care أول
+
+403
+00:29:59,680 --> 00:30:04,120
+إشي بدي أتبع و أقرأ بعناية ال manufacturer
+
+404
+00:30:04,120 --> 00:30:10,090
+instructionو طبعا لازم اتأكد ان ال incubator موجود
+
+405
+00:30:10,090 --> 00:30:15,510
+على ال .. ال position on a level surface يكون يعني
+
+406
+00:30:15,510 --> 00:30:21,490
+مستوي على بنش مستوي و مايكون في انسداد لنظام
+
+407
+00:30:21,490 --> 00:30:26,490
+التهوية لإله يعني مايكون خابط في الحيط ورا عاملى
+
+408
+00:30:26,490 --> 00:30:32,540
+block لنظام التهوية لإن هو بياخد هوا و ..بيدفيه
+
+409
+00:30:32,540 --> 00:30:37,620
+لدرجة حرارة 37 و بيخلي الوسط كله هنا 37 و هنا زي
+
+410
+00:30:37,620 --> 00:30:41,460
+ما انتوا شايفين فيه زي thermometer عشان نتأكد ان
+
+411
+00:30:41,460 --> 00:30:44,380
+درجة الحرارة جوا زي اللي موجودة على الشاشة برا
+
+412
+00:30:47,090 --> 00:30:50,610
+طمام if the incubator doesn't have temperature
+
+413
+00:30:50,610 --> 00:30:54,290
+display insert a thermometer لو كان مافيش عندي هنا
+
+414
+00:30:54,290 --> 00:31:01,430
+شاشة لحتى تبينلي درجة الحرارة ممكن انا احط
+
+415
+00:31:01,430 --> 00:31:05,020
+thermometer في الداخل و اضل اراقبهbefore
+
+416
+00:31:05,020 --> 00:31:07,280
+incubating culture and test it check the
+
+417
+00:31:07,280 --> 00:31:10,920
+temperature حكينا قبل ال incubation لازم أشيك على
+
+418
+00:31:10,920 --> 00:31:16,240
+درجة الحرارة لازم أنضفه باستمرار يعني بعد ما أقرأ
+
+419
+00:31:16,240 --> 00:31:20,260
+الأطباق هاي و أخلص استخدامتيها لازم أطلعها على
+
+420
+00:31:20,260 --> 00:31:24,990
+التعقيماعدام يعني بتروح اعدام على جهاز التعقيم
+
+421
+00:31:24,990 --> 00:31:28,530
+بنقتل كل البكتيريا و الميكروبات اللى موجودة لضمان
+
+422
+00:31:28,530 --> 00:31:35,370
+عدم نقل العدوى او نقل الجراثيم و .. و بنضف الجهاز
+
+423
+00:31:35,370 --> 00:31:41,130
+باستمرار لحتى انا اضل محافظة عليه نظيف و لضمان
+
+424
+00:31:41,130 --> 00:31:42,150
+سلامتى كمان
+
+425
+00:31:46,160 --> 00:31:51,780
+طبعا و أنا بنطفه لازم أتأكد أن هو مفصول من الكهربا
+
+426
+00:31:51,780 --> 00:31:55,180
+علشان أبقى محافظة على نفسي Instruments for
+
+427
+00:31:55,180 --> 00:32:00,360
+purifying water اللي هو عبارة عن أجهزة لطنقية
+
+428
+00:32:00,360 --> 00:32:05,780
+الماء تمام؟ في عندي أول حاجة طبعا جودة الماء مهمة
+
+429
+00:32:05,780 --> 00:32:11,140
+جدا is very crucial أساسية جدا ليش؟ لأن الماء هو
+
+430
+00:32:11,140 --> 00:32:16,250
+the most common solventused in medical
+
+431
+00:32:16,250 --> 00:32:20,050
+laboratories هو يعني أنا بدأ أحضر saline بدوّب ملح
+
+432
+00:32:20,050 --> 00:32:23,350
+في distilled water لو بدأ أحضر سبغة بدوّب بال
+
+433
+00:32:23,350 --> 00:32:27,150
+powder تبع السبغة بدistilled water لو بدأ أحضر
+
+434
+00:32:27,150 --> 00:32:31,150
+buffer بدي أدوّب ال base و ال acid بدistilled
+
+435
+00:32:31,150 --> 00:32:34,570
+water لو بدي أحضر reagent حكيتلكم ال reagent ممكن
+
+436
+00:32:34,570 --> 00:32:37,910
+ييجي عبارة عن powder و أدوبه في distilled waterإذا
+
+437
+00:32:37,910 --> 00:32:41,630
+لو بدي عندي محلول مركز و بدي أخففه بقى أخفف ب
+
+438
+00:32:41,630 --> 00:32:45,310
+distilled water إذا ال water هو ال most common
+
+439
+00:32:45,310 --> 00:32:50,790
+solvent هو ال solvent أو المضيب اللي هو اللي أكتر
+
+440
+00:32:50,790 --> 00:32:55,790
+شيوعا أو أكتر استخداما بال medical laboratories
+
+441
+00:32:56,210 --> 00:33:00,190
+طبعا بيستخدم في تحضير ال standard solutions لل
+
+442
+00:33:00,190 --> 00:33:05,830
+buffers لل controls طبعا مثلا ال standard solution
+
+443
+00:33:05,830 --> 00:33:13,290
+اللي تركيزه كان 40x في فحص السكر طبعا كان ..
+
+444
+00:33:13,290 --> 00:33:20,060
+تركيزه 400مش اربعين extra اربعمية طبعا و انا لازم
+
+445
+00:33:20,060 --> 00:33:23,700
+اوصله لمية اللي هو ال working solution ال working
+
+446
+00:33:23,700 --> 00:33:29,200
+standard solution لما بدي استخدمه لازم اخففه ب ..
+
+447
+00:33:29,200 --> 00:33:33,010
+ب distilled water طبعاال buffers أيضا تحضيرها
+
+448
+00:33:33,010 --> 00:33:39,910
+حكينا ال controls اللي هي العينات الضابطة الصبغات
+
+449
+00:33:39,910 --> 00:33:43,990
+المحالي لل reagents في سواء كان في قسم ال
+
+450
+00:33:43,990 --> 00:33:48,230
+chemistry في المناعة في ال hematologyفي ال
+
+451
+00:33:48,230 --> 00:33:53,010
+microbiology اللي هو علم الميكروبات والجرافيم في
+
+452
+00:33:53,010 --> 00:33:57,650
+كل الأقسام أنا بستخدم distilled water ك .. ك ..
+
+453
+00:33:57,650 --> 00:34:02,490
+يعني مثيب أو solution لتحضير أو كبيس كاساس يعني
+
+454
+00:34:02,940 --> 00:34:06,860
+لتحضير ال reagents طبعا و ايضا كمان ال culture
+
+455
+00:34:06,860 --> 00:34:11,700
+media هي المادة المغذية اللى بتنمي البكتيريا و
+
+456
+00:34:11,700 --> 00:34:16,840
+الفطريات و لزراعة الخلايا ايضا بتكون برضه كمان
+
+457
+00:34:16,840 --> 00:34:20,140
+عبارة عن مواد مغذية in distilled water يعني لما
+
+458
+00:34:20,140 --> 00:34:23,960
+بدى احضر media للبكتيريا ولا للفطريات بوزن ال ..
+
+459
+00:34:23,960 --> 00:34:27,340
+البودر و بدوبه في distilled water بفوته يتعقم و
+
+460
+00:34:27,340 --> 00:34:33,860
+بعدين بفضيه في أطباق علشان أزرع عليها العيناتتمام؟
+
+461
+00:34:33,860 --> 00:34:40,160
+فال .. ال reagent اللي بتستخدم في ال blood is
+
+462
+00:34:40,160 --> 00:34:44,400
+transfusion work فشغل اللي هو نقل الدم برضه كمان
+
+463
+00:34:44,400 --> 00:34:49,320
+محتاجة distilled water فبالتالي إذا ال water يعني
+
+464
+00:34:49,320 --> 00:34:56,340
+يستخدم فيه كتير مجالات في حال .. أحيانا بالأجهزةلو
+
+465
+00:34:56,340 --> 00:35:01,660
+بدي أنظف تيوب بده .. أحيانا في بعض الأجهزة خصوصا
+
+466
+00:35:01,660 --> 00:35:05,420
+الأجهزة اللي بتسحب العينة سحب لازم ال .. ده اللي
+
+467
+00:35:05,420 --> 00:35:10,060
+بتسحب اللي فيها لازم يسيللها rinsing أشرنزنج يعني
+
+468
+00:35:10,060 --> 00:35:13,560
+غمر بغسول أو بمضيب يعني ان احنا ننظفها ما بين كل
+
+469
+00:35:13,560 --> 00:35:19,320
+عينة و التانيةأيضا نحتاج ال .. ال water شايفين كام
+
+470
+00:35:19,320 --> 00:35:24,040
+هي مهمة ال water؟ فلازم أيضا الأجهزة اللي ت .. ال
+
+471
+00:35:24,040 --> 00:35:30,720
+.. التحضير و الفصل و التعقيم تكون مهمة في عندي
+
+472
+00:35:30,720 --> 00:35:34,220
+جهاز اللي هو ال water distilling apparatus اللي هو
+
+473
+00:35:34,220 --> 00:35:41,300
+يعني فكرة ال .. ال .. الفلترة التنقية و الفلترة
+
+474
+00:35:41,300 --> 00:35:47,570
+للماءطبعا هي فكرة بسيطة بتعتمد على تسخين الماء و
+
+475
+00:35:47,570 --> 00:35:52,170
+.. فبالتالي ال .. ال .. ال .. يعني هو الماء غير
+
+476
+00:35:52,170 --> 00:35:57,650
+نقيب بيتعرض لحرارة بيسخن البخار بيتكاثف و بيتم
+
+477
+00:35:57,650 --> 00:36:03,250
+يعني نقله من خ .. لما بيتكاثف بيتم نقله إلى وعاء
+
+478
+00:36:03,250 --> 00:36:12,470
+نظيف فال .. ال ..هذا الماء المتكاثف طبعا هو اللي
+
+479
+00:36:12,470 --> 00:36:14,810
+.. اللي بنعتبره distilled water لإنه خالي من
+
+480
+00:36:14,810 --> 00:36:19,190
+الشوائب و الأملح تمام؟ لإنه لما اتبخر ما اتبخرت
+
+481
+00:36:19,190 --> 00:36:22,970
+معاه الأملح و الشوائب اللي موجودة فيه و لما تكاثف
+
+482
+00:36:22,970 --> 00:36:28,270
+نتيجة يعني وجوده في .. على سطح .. على أسطح باردة
+
+483
+00:36:28,270 --> 00:36:33,230
+تمام؟ يعني ماكان موجود فيه هاي الشوائب لذلك هيك
+
+484
+00:36:33,230 --> 00:36:37,650
+بنكون احنا حصلنا على distilled water تمام؟إذن
+
+485
+00:36:37,650 --> 00:36:42,230
+water distilling apparatus is an instrument that
+
+486
+00:36:42,230 --> 00:36:46,890
+used to purify impure water هو عبارة عن جهاز
+
+487
+00:36:46,890 --> 00:36:52,910
+لتنقية ال .. to purify تنقية ال impure water اللي
+
+488
+00:36:52,910 --> 00:36:56,370
+هو الماء الغير نقي by process known as
+
+489
+00:36:56,370 --> 00:36:59,770
+distillation من خلال عملية اسمها distillation اللي
+
+490
+00:36:59,770 --> 00:37:03,980
+احنا شرحنا مبدأها حالياdistillation هو عبارة عن
+
+491
+00:37:03,980 --> 00:37:07,420
+process by which impure water is boiled، بنغلي
+
+492
+00:37:07,420 --> 00:37:12,320
+الميه اللى هى ال impure، اللى هى الغير نقية، تمام؟
+
+493
+00:37:12,320 --> 00:37:17,120
+و بخارها بيتم نقله الى أنابيب أخرى، تمام؟ هذه
+
+494
+00:37:17,120 --> 00:37:19,980
+الأنابيب مكثفة، ليش؟ لإنها بتكون طبعا ال ..
+
+495
+00:37:23,380 --> 00:37:30,340
+البخار هذا بيكون موجود في أنابيب باردة نوعا ما
+
+496
+00:37:30,340 --> 00:37:35,600
+فبيتكاثف وبيتم نقله ك pure water تمام؟ even the
+
+497
+00:37:35,600 --> 00:37:38,900
+steam produced condensed in a cold surface بيتم
+
+498
+00:37:38,900 --> 00:37:43,220
+تكاثفه على أسطح باردة اللي هي الcondenser وطبعا
+
+499
+00:37:43,220 --> 00:37:47,120
+بيعطيني chemically pure distilled waterيعني ماء
+
+500
+00:37:47,120 --> 00:37:52,840
+خالي من الشوائب الكيميائية طبعا that is water from
+
+501
+00:37:52,840 --> 00:37:56,920
+which non-volatile organic and inorganic materials
+
+502
+00:37:56,920 --> 00:38:01,320
+are removed طبعا المواد الغير متطايرة اللي موجودة
+
+503
+00:38:01,320 --> 00:38:04,380
+سواء كانت عضوية أو غير عضوية هيك بنكون اتخلصنا
+
+504
+00:38:04,380 --> 00:38:11,150
+منها تمام؟من مساوئ هذه الطريقة إن هي ما بتخلصني من
+
+505
+00:38:11,150 --> 00:38:15,150
+ال ionic gases اللي موجودة في الماء زي إذا كان
+
+506
+00:38:15,150 --> 00:38:18,010
+موجود في ammonia أو carbon dioxide أو chlorine
+
+507
+00:38:18,010 --> 00:38:25,810
+طبعا ما بتخلصني منها و أيضا أحيانا ممكنتكون الماء
+
+508
+00:38:25,810 --> 00:38:28,050
+اللي هي ال distilled water اللي انا حصلت عليها
+
+509
+00:38:28,050 --> 00:38:30,650
+contaminated with non volatile impurities يعني
+
+510
+00:38:30,650 --> 00:38:36,430
+يكون فيها بعض الشوائب الغير متطايرة زي ال sodium و
+
+511
+00:38:36,430 --> 00:38:38,850
+ال potassium و ال calcium و ال carbonate ion و ال
+
+512
+00:38:38,850 --> 00:38:43,930
+sulphate ion طبعا فلذلك ممكن انا استخدم اذا بدي
+
+513
+00:38:43,930 --> 00:38:47,910
+يعني ماء درجة نقاطه اعلى بكتير ممكن استخدم اجهزة
+
+514
+00:38:47,910 --> 00:38:51,410
+تانية غير ال distillator في عندي جهاز اللي هو ال
+
+515
+00:38:51,410 --> 00:38:58,670
+gravity water filterال filtration هو عبارة عن the
+
+516
+00:38:58,670 --> 00:39:02,970
+passage of liquid مرور الماء أو السائل من خلال
+
+517
+00:39:02,970 --> 00:39:07,630
+filter وبيتضمن مروره طبعا لازم من خلال gravity
+
+518
+00:39:07,630 --> 00:39:13,330
+جاذبية أو حتى pressure أو ضغط يعني تمام؟ فبالتالي
+
+519
+00:39:13,330 --> 00:39:21,010
+بيت filter و بيتم ..يعني حصول على الماء نقي نوعا
+
+520
+00:39:21,010 --> 00:39:24,710
+ما طيب هنا زي مثلا هذا الجهاز ال gravity water
+
+521
+00:39:24,710 --> 00:39:28,730
+filter طبعا هذا inlet يعني من هنا بيدخل الماء
+
+522
+00:39:28,730 --> 00:39:34,530
+الغير نقي تمام او المراد فلترته و بيمر على فلاتر
+
+523
+00:39:34,530 --> 00:39:37,610
+يعني الفلاتر هذه تتبين في أحجامها يعني مثلا هنا في
+
+524
+00:39:37,610 --> 00:39:42,260
+عندي سخوريعني المسافات البينية كبيرة بعدين بييجي
+
+525
+00:39:42,260 --> 00:39:47,000
+هنا في عندي perforated metal plate يعني مادة
+
+526
+00:39:47,000 --> 00:39:51,620
+معدنية مثقبة بيمر من خلالها الماء بيمر على ال ..
+
+527
+00:39:51,620 --> 00:39:56,620
+على رمل تمام و احنا بنعرف ان الرمل يستخدم ك filter
+
+528
+00:39:56,620 --> 00:40:00,320
+يعني الماء اللي .. اللي بيطلع من الينابيع من الرمل
+
+529
+00:40:00,320 --> 00:40:07,400
+بيكون كتير نقي لإن الرمل ممكن يشغل ك filter تمام؟
+
+530
+00:40:08,750 --> 00:40:12,770
+فهنا بالنهاية بيطلع عندي .. بيبدأ يرتفع منسوب ال
+
+531
+00:40:12,770 --> 00:40:17,790
+.. ال water كل ما ضغطنا ماء أكتر، تمام؟ بيدخل
+
+532
+00:40:17,790 --> 00:40:20,970
+الماء و بنضغط عليه إما ب .. يعني بإما بينزل بفعل
+
+533
+00:40:20,970 --> 00:40:23,930
+الجاذبية أو أنا ب .. بعمل apply pressure يعني
+
+534
+00:40:23,930 --> 00:40:27,250
+بيكون في عندي ضغط على ال ..المقفة بيرتفع في ..
+
+535
+00:40:27,250 --> 00:40:30,890
+بيبدأ يطلع، هي عبر من خلال ال rock، من خلال ال ..
+
+536
+00:40:30,890 --> 00:40:34,470
+ال .. المعدن، من خلال ال sand و بيبدأ يرتفع كفي
+
+537
+00:40:34,470 --> 00:40:41,590
+يعني يرشح على سطح ال sand كماء مفلتر أو يعني منقه
+
+538
+00:40:41,590 --> 00:40:45,340
+و بعد هيك بيخرج من خلال ال outlet، تمام؟ال
+
+539
+00:40:45,340 --> 00:40:48,640
+filtrate هذا طبعا ال .. ال .. ال .. ناتج الفلترة
+
+540
+00:40:48,640 --> 00:40:52,780
+بسميه filtrate، طبعا؟ وهذا بدي .. المصطلح هتستخدمه
+
+541
+00:40:52,780 --> 00:40:58,580
+كتير في التحليل، يعني هن .. هن filter محليل ون
+
+542
+00:40:58,580 --> 00:41:02,320
+filter ميه ون filter صبغات ون filter أشياء كتير،
+
+543
+00:41:02,320 --> 00:41:07,300
+فالناتج الفلترة بسميه filtrate هو عبارة عن ال
+
+544
+00:41:07,300 --> 00:41:12,440
+liquid that has passed through the filterإذا
+
+545
+00:41:12,440 --> 00:41:17,760
+المحلول اللي خرجوا أو عدّى من خلال ال filter بسميه
+
+546
+00:41:17,760 --> 00:41:22,000
+filtrate the purpose of filtration طبعا إيش وظيفة
+
+547
+00:41:22,000 --> 00:41:25,000
+ال filtration؟ to remove particulate matter يعني
+
+548
+00:41:25,000 --> 00:41:30,570
+لحتى يعني أتخلص من الشوائب و المواد الكبيرةنوعا ما
+
+549
+00:41:30,570 --> 00:41:35,690
+زي ال bacteria و ال parasite و ال microorganism زي
+
+550
+00:41:35,690 --> 00:41:40,470
+لو ماكان فيه شوائب أو يعني أوساخ في داخل الماء
+
+551
+00:41:40,470 --> 00:41:45,630
+ممكن أتخلص منها بهذا الشكل لكن مابتخلصش من خلالمن
+
+552
+00:41:45,630 --> 00:41:49,350
+خلال هذه العملية من ال dissolve salt يعني الأملاح
+
+553
+00:41:49,350 --> 00:41:53,070
+اللي دائبة تمام؟ يعني ممكن أتخلص من بعض الأملاح من
+
+554
+00:41:53,070 --> 00:41:57,090
+خلال مثلا إما ال distillation .. عملية ال
+
+555
+00:41:57,090 --> 00:42:00,990
+distillation أو من خلال اللي هي ال de-ionization
+
+556
+00:42:00,990 --> 00:42:05,750
+تمام؟طيب جديد بالذكر أنه أنا حكيتلكم مثلا إذا كان
+
+557
+00:42:05,750 --> 00:42:11,710
+أنا محتاجة تنقي أكثر ممكن أستخدم أجهزة بتنقي أكثر
+
+558
+00:42:11,710 --> 00:42:16,270
+و أحيانا ممكن أنا أحصل على يعني لحتى أضمن أنه
+
+559
+00:42:16,270 --> 00:42:22,000
+الماء مفلتر جيداأعمل double distillation و كتير
+
+560
+00:42:22,000 --> 00:42:25,460
+أحيانا بنحتاج ال double distilled water يعني ماء
+
+561
+00:42:25,460 --> 00:42:29,800
+مقطر مرتين يعني الماء المقطر اللي هنا أنا رجعت
+
+562
+00:42:29,800 --> 00:42:34,040
+غليته مرتانة و إكثفته، تمام؟ هذا بسميه double
+
+563
+00:42:34,040 --> 00:42:38,600
+distilled water خصوصا في الفحوصات اللي هي ال .. ال
+
+564
+00:42:38,600 --> 00:42:43,940
+molecular في فحوصات ال molecular اللي هي الفحوصات
+
+565
+00:42:43,940 --> 00:42:48,370
+الجينية أنا أحيانا بشتغل بإنزيماتأي تركيز لأملاح
+
+566
+00:42:48,370 --> 00:42:52,330
+أو معادن في داخل الميه ممكن يأثر على تنشاط هذه
+
+567
+00:42:52,330 --> 00:42:57,390
+الانزيمات فبالتالي بكون محتاجة double distilled
+
+568
+00:42:57,390 --> 00:43:04,350
+waterطب ال deionizer إيش يعني نازع للأيونات؟ دي
+
+569
+00:43:04,350 --> 00:43:10,710
+نازع، تمام؟ ionizer يعني نازع للأيونات، تمام؟
+
+570
+00:43:10,710 --> 00:43:16,390
+deionizer هو عبارة عن apparatus to produce ion
+
+571
+00:43:16,390 --> 00:43:21,510
+-free water، تمام؟ لحتى تنتجلي ماء خالي أو يعني
+
+572
+00:43:21,510 --> 00:43:27,570
+free of ions، تمام؟ إذا عملية ال deionization
+
+573
+00:43:30,320 --> 00:43:35,720
+هنا deionization تمام هي عملية is a process in
+
+574
+00:43:35,720 --> 00:43:40,400
+which chemically impure water الماء اللي غير نقي
+
+575
+00:43:40,400 --> 00:43:46,760
+فيه شوائب كيميائية تمام بيمر على anion and cation
+
+576
+00:43:46,760 --> 00:43:51,740
+risen تمام و بالنهاية أنا بحصل على ion-free water
+
+577
+00:43:51,740 --> 00:43:56,760
+إيش يعني anion and cation risen يعني عبارة عن ..
+
+578
+00:43:56,760 --> 00:44:02,310
+يعني زي أقطابفيها انيونات وفيها كاتيونات، تمام؟
+
+579
+00:44:02,310 --> 00:44:06,370
+هلأ لما .. بس نشوف الصورة هنوضح أكتر أو هتتضح
+
+580
+00:44:06,370 --> 00:44:12,010
+الفكرة أكتر Deionized water has low electrical
+
+581
+00:44:12,010 --> 00:44:17,500
+conductivityقليلة أو شبه معضومة ليش؟ لإن إحنا
+
+582
+00:44:17,500 --> 00:44:20,460
+نزعنا الأيونات اللي فيها هو إيش اللي بيخلي الماء
+
+583
+00:44:20,460 --> 00:44:23,740
+وموصل للكهربا غير الأيونات اللي بتكون موجودة فيه
+
+584
+00:44:23,740 --> 00:44:31,530
+near neutral pH بيكون ال pH متعادل about 7و طبعا
+
+585
+00:44:31,530 --> 00:44:35,210
+free from water soluble salt بيكون خالي من الأملاح
+
+586
+00:44:35,210 --> 00:44:39,310
+و خالي من الأيونات بس ممكن ما بيكون معقّم تمام أنا
+
+587
+00:44:39,310 --> 00:44:42,730
+بحتاج أفوته جهاز تعقيم عشان أعقمه بعد ما أنزع
+
+588
+00:44:42,730 --> 00:44:46,330
+الأيونات منه لو أنا محتاجة sterile and deionized
+
+589
+00:44:46,330 --> 00:44:50,250
+water لكن إذا محتاجة deionized water لحال فخلاص
+
+590
+00:44:50,250 --> 00:44:55,510
+بمرره من خلال جهاز ال deionizer و بعدين بستخدمه
+
+591
+00:44:55,510 --> 00:45:02,070
+طيبال cations اللي بتكون ممكن تكون موجودة في الماء
+
+592
+00:45:02,070 --> 00:45:07,890
+which may be present in water زي ash يعني أجزاء
+
+593
+00:45:07,890 --> 00:45:11,910
+مشحونة موجبة cations يعني هتكون زي ال calcium، ال
+
+594
+00:45:11,910 --> 00:45:16,580
+magnesium، ال sodium، تمام؟و هذه أيضًا بيتم ..
+
+595
+00:45:16,580 --> 00:45:20,260
+يعني بيه .. احنا حكينا هتمر على كاتيون و أنيون
+
+596
+00:45:20,260 --> 00:45:25,720
+risen تمام؟ فهتمر على الكاتيون و ياخدها الكاتيون
+
+597
+00:45:25,720 --> 00:45:31,070
+تبع الجهاز و يقطي الماء كاتيون آخروال onions اللى
+
+598
+00:45:31,070 --> 00:45:34,790
+بتكون فى الماء زى الصلفات و ال bicarbonate و ال
+
+599
+00:45:34,790 --> 00:45:38,650
+silicate تمام و ال nitrate و ال chloride أي أجزاء
+
+600
+00:45:38,650 --> 00:45:42,910
+شحنتها سالبة بيتم استبدالها بانيون أخرى من الجهاز
+
+601
+00:45:42,910 --> 00:45:47,730
+يعنى مثلا هى هنا الجهاز موجود فى cation و موجود فى
+
+602
+00:45:47,730 --> 00:45:51,550
+anion resin ال cation resin بيكون موجود فى فقط
+
+603
+00:45:51,550 --> 00:45:55,170
+أيودات ال hydrogen تمام ال protonate يعنى بيكون
+
+604
+00:45:55,170 --> 00:45:59,520
+موجود فىهو ال .. ال .. ال anion resin تبع الجهاز
+
+605
+00:45:59,520 --> 00:46:04,500
+بيكون فيه OH، تمام؟ بيكون فيه OH، هلأ مجرد ما أنا
+
+606
+00:46:04,500 --> 00:46:07,960
+أمرر الماء، هي قطرة الماء اللي مرت مثلا، بنعتبر
+
+607
+00:46:07,960 --> 00:46:10,940
+إنه هذه كمية الماء اللي أنا بدي أعمل لها de
+
+608
+00:46:10,940 --> 00:46:14,180
+-ionization، هلأ هي موجود فيها أنيونات زي ال
+
+609
+00:46:14,180 --> 00:46:17,760
+sodium و موجود فيها .. sorry موجود فيها أنيونات زي
+
+610
+00:46:17,760 --> 00:46:22,640
+ال chloride و كتيونات زي ال sodiumطبعا طيب مرت على
+
+611
+00:46:22,640 --> 00:46:26,660
+ال reason تبع الجهاز اللي هو عبارة عن إيش كتيوناتو
+
+612
+00:46:26,660 --> 00:46:32,280
+هيدروجين و أنيوناتو هيدروكسل طبعا فإيش هيعمل
+
+613
+00:46:32,280 --> 00:46:37,760
+الجهاز؟ هيروح ماخد الصوديوم من الماء ومعطيها بداله
+
+614
+00:46:37,760 --> 00:46:44,220
+اللي هو H plusطبعا استبدال كتاين بكتاين أخد صوديوم
+
+615
+00:46:44,220 --> 00:46:49,120
+و أعطى الماء اتش طبعا فهنا إيش حكينا الكتاين زى
+
+616
+00:46:49,120 --> 00:46:53,020
+اللى فى الجهاز اللى زى الصوديوم صار إلها exchange
+
+617
+00:46:53,020 --> 00:46:58,840
+تم تبديلها بإيش بكتاين من وين من الرزن من الجهاز
+
+618
+00:46:58,840 --> 00:47:06,160
+اللى عبارة عن hydrogen ions طبعا طيبوالجهاز بياخد
+
+619
+00:47:06,160 --> 00:47:11,220
+ال anions اللي هي زي ال chloride المشحونة سالبة و
+
+620
+00:47:11,220 --> 00:47:16,780
+بيروح بيعطي الماء اش بدالها OH برضه مشحونة سالبة،
+
+621
+00:47:16,780 --> 00:47:21,480
+اذا اللي هي ال anions اللي موجودة في الماء زي مين؟
+
+622
+00:47:21,480 --> 00:47:28,060
+زي ال chlorideتمام؟ بيصيرلها exchange بإيش؟
+
+623
+00:47:28,060 --> 00:47:35,440
+بالانيون تبع الرزن تبع الجهاز تمام؟ و ال .. ال ..
+
+624
+00:47:35,440 --> 00:47:37,060
+ال .. ال .. ال .. ال .. ال .. ال .. ال .. ال .. ال
+
+625
+00:47:37,060 --> 00:47:37,080
+.. ال .. ال .. ال .. ال .. ال .. ال .. ال .. ال ..
+
+626
+00:47:37,080 --> 00:47:37,240
+ال .. ال .. ال .. ال .. ال .. ال .. ال .. ال .. ال
+
+627
+00:47:37,240 --> 00:47:37,520
+.. ال .. ال .. ال .. ال .. ال .. ال .. ال .. ال ..
+
+628
+00:47:37,520 --> 00:47:37,920
+ال .. ال .. ال .. ال .. ال .. ال .. ال .. ال .. ال
+
+629
+00:47:37,920 --> 00:47:38,820
+.. ال .. ال .. ال .. ال .. ال .. ال .. ال .. ال ..
+
+630
+00:47:38,820 --> 00:47:39,260
+ال .. ال .. ال .. ال .. ال .. ال .. ال .. ال .. ال
+
+631
+00:47:39,260 --> 00:47:39,340
+.. ال .. ال .. ال .. ال .. ال .. ال .. ال .. ال ..
+
+632
+00:47:39,340 --> 00:47:39,460
+ال .. ال .. ال .. ال .. ال .. ال .. ال .. ال .. ال
+
+633
+00:47:39,460 --> 00:47:39,520
+.. ال .. ال .. ال .. ال .. ال .. ال .. ال .. ال ..
+
+634
+00:47:39,520 --> 00:47:45,420
+ال .. ال .. ال .. ال .. ال .. ال .. ال .. الفقد ال
+
+635
+00:47:45,420 --> 00:47:50,720
+sodium و فقد ال chloride كل منهم راح على ال resin
+
+636
+00:47:50,720 --> 00:47:56,140
+اللي خاص بشحنته تمام؟ و اكتسب بدلهم HOH طب ما هدول
+
+637
+00:47:56,140 --> 00:48:00,200
+ال HOH بيصيروا لهم combine مع بعض، اذا ال hydrogen
+
+638
+00:48:00,200 --> 00:48:03,920
+ion و ال .. و ال hydroxyl بيصيروا لهم combine مع
+
+639
+00:48:03,920 --> 00:48:08,360
+بعض و بيعطونيwater إذا في النهاية أنا حصلت على
+
+640
+00:48:08,360 --> 00:48:13,540
+water بطريقة استبدال الأيونات وخالية من الأيونات
+
+641
+00:48:13,540 --> 00:48:16,980
+الأصلية اللي كانت موجودة في ال water الأصلي، تمام؟
+
+642
+00:48:16,980 --> 00:48:20,580
+هذا ال water اللي أنا حصلت عليه هو عبارة عن de
+
+643
+00:48:20,580 --> 00:48:26,610
+-ionized water، ماء منزوع الأيونات، تمام؟resin إيش
+
+644
+00:48:26,610 --> 00:48:31,070
+معناه كلمة resin؟ هي particles أجزاء على ال .. في
+
+645
+00:48:31,070 --> 00:48:35,710
+ال .. يعني أجزاء بتكون قادرة إنها تحمل شحنة طبعا؟
+
+646
+00:48:35,710 --> 00:48:40,250
+مواد يعني قادرة إنها تحمل شحنة أو تحمل آيونات فهي
+
+647
+00:48:40,250 --> 00:48:45,710
+مواد بنسميها resin مادة بتحمل على سطحها البروتونات
+
+648
+00:48:45,710 --> 00:48:48,830
+هذه أو ال hydrogen ion في حالة .. فبنسميها
+
+649
+00:48:48,830 --> 00:48:53,150
+cationic resin طبعا؟ particle بتكون شايلة ال
+
+650
+00:48:53,150 --> 00:48:58,400
+hydroxylIon تمام فبسميها اللي هو ال anionic rhythm
+
+651
+00:48:58,400 --> 00:49:03,160
+أو ممكن أسميها electrode تمام يعني مثلا هنا .. هاي
+
+652
+00:49:03,160 --> 00:49:06,420
+هنا مثلا ال .. ال .. في ناحية ال positive و هدا من
+
+653
+00:49:06,420 --> 00:49:10,330
+ناحية ال negative يعني هاد ال cationicتمام؟ وهذا
+
+654
+00:49:10,330 --> 00:49:14,750
+ال onionic فاش اللي هيصير بالظبط الماء هيفوت هيروح
+
+655
+00:49:14,750 --> 00:49:18,350
+مثلا ال sodium باتجاه من .. باتجاه ال cationic
+
+656
+00:49:18,350 --> 00:49:22,310
+ويجي بدله hydrogen و ال chloride اللي في الماء
+
+657
+00:49:22,310 --> 00:49:27,970
+مثلا او اي onions موجودة شحنتها سالبة هتروح باتجاه
+
+658
+00:49:27,970 --> 00:49:33,450
+السالب ويتم استبدالها بسالب بدالها تمام؟عشان ما
+
+659
+00:49:33,450 --> 00:49:39,050
+يأثر على شحنة الماء ككل ففي الت .. بالتالي او ..
+
+660
+00:49:39,050 --> 00:49:42,170
+او sorry يعني هو في النهاية احنا معنين انه شحنة ال
+
+661
+00:49:42,170 --> 00:49:47,270
+electrode نفسه ما تتغير ككل طبعا ففي النهاية بيتم
+
+662
+00:49:47,270 --> 00:49:51,350
+نزع ال sodium و ال chloride ب .. باستخدام ال resin
+
+663
+00:49:51,350 --> 00:49:55,670
+هدول واستبدالهم ب hydrogen و hydroxyl و هدول
+
+664
+00:49:55,670 --> 00:49:59,470
+بيعطونى مية اذا انا حصلت على ماء خالي من الايونار
+
+665
+00:49:59,470 --> 00:50:03,290
+بهذه الفكرةتمام؟ هذه فكرة عمل ال .. ال .. ال
+
+666
+00:50:03,290 --> 00:50:12,330
+ionizer هيك بنكون وصلنا لنهاية المحاضرة و أيضا
+
+667
+00:50:12,330 --> 00:50:18,690
+لختم محاضرات مساق المقدمة في التحاليل الطبية أتمنى
+
+668
+00:50:18,690 --> 00:50:24,690
+أنكم تكونوا استفدتم و استمتعتم أتمنى ذلك انه في ..
+
+669
+00:50:24,690 --> 00:50:30,130
+بالمساق و ..ان شاء الله يعني بتطلعوا منه بتكونوا
+
+670
+00:50:30,130 --> 00:50:35,290
+على خبرة و دراية كافية اتمكنكم من انكم تاخدوا
+
+671
+00:50:35,290 --> 00:50:39,870
+المثاقات الأخرى اللى بتعتمد على هذا المثاق و اللى
+
+672
+00:50:39,870 --> 00:50:43,610
+من خلاله أيضا يعني انتوا هتنطلقوا الى التخصصات
+
+673
+00:50:43,610 --> 00:50:47,830
+الأخرى اللى موجودة تحت مسمة علوم طبية مخبرية
+
+674
+00:50:50,320 --> 00:50:57,220
+رح أطالب م .. أطالبكم بواجب أنه تختاري جهاز يستخدم
+
+675
+00:50:57,220 --> 00:51:03,740
+بال medical laboratories، بالمختبرات الطبية، طبعا
+
+676
+00:51:03,740 --> 00:51:07,230
+الجهاز سواء كان incubator، ovenbalance,
+
+677
+00:51:08,170 --> 00:51:13,750
+centrifuge, spectrohotometer او حتى اي جزء اخر
+
+678
+00:51:13,750 --> 00:51:21,330
+احنا ما ذكرناها مثلا ممكن انك يعني تختاريها و
+
+679
+00:51:21,330 --> 00:51:29,250
+تبحثي عنها تكتبيلي عن فكرة عملها و استخدامها و
+
+680
+00:51:29,250 --> 00:51:36,850
+مبدأ عملها بشكل يعني مبسط تماميعني بدي واجب هيك من
+
+681
+00:51:36,850 --> 00:51:41,230
+صفحة لصفحة و نص عن جهاز يستخدم في ال medical
+
+682
+00:51:41,230 --> 00:51:44,330
+laboratories سواء من اللي ذكرناهم في المساق أو
+
+683
+00:51:44,330 --> 00:51:49,290
+سواء إذا إنتي واجهتي أثناء بحتك جهاز ما اتطرقناله
+
+684
+00:51:49,290 --> 00:51:53,350
+ممكن أنه أيضا كمان يتغطي من خلال هذا ال report أو
+
+685
+00:51:53,350 --> 00:51:59,770
+هذا ال homework بتمنعكم التوفيق يعطيكم ألف عافية و
+
+686
+00:51:59,770 --> 00:52:00,910
+هى ان شاء الله موفقين
+
diff --git a/PL9fwy3NUQKwY9jl8xw-bWkVZkWcGGyspd/S5RQk6QZXf4.srt b/PL9fwy3NUQKwY9jl8xw-bWkVZkWcGGyspd/S5RQk6QZXf4.srt
new file mode 100644
index 0000000000000000000000000000000000000000..5b718b311c8a87b75e1f783486787455dc3d21d3
--- /dev/null
+++ b/PL9fwy3NUQKwY9jl8xw-bWkVZkWcGGyspd/S5RQk6QZXf4.srt
@@ -0,0 +1,2642 @@
+1
+00:00:04,520 --> 00:00:07,580
+السلام عليكم ورحمة الله وبركاته. طالبتي، اليوم إن شاء الله
+
+2
+00:00:07,580 --> 00:00:12,060
+راح نأخذ معملنا رقم اثنين بعنوان automatic
+
+3
+00:00:12,060 --> 00:00:15,600
+pipette and calibration. معمل رقم اثنين، فيه مساق
+
+4
+00:00:15,600 --> 00:00:20,760
+مقدمة في التحليل الطبي. طبعاً هذا كان أول موضوع كان
+
+5
+00:00:20,760 --> 00:00:25,100
+المفروض أن احنا نأخذه عملياً، ونشتغل بأدواتنا، ولكن
+
+6
+00:00:25,100 --> 00:00:30,440
+للأسف، نظراً للظروف المحيطة، فنحن راح نأخذ إن شاء الله
+
+7
+00:00:30,440 --> 00:00:33,660
+... راح نأخذ النظريّ تبعه، وإن شاء الله بعودتنا
+
+8
+00:00:33,660 --> 00:00:38,460
+للمختبرات نأخذ التطبيق العملي له. فاليوم إن شاء الله
+
+9
+00:00:38,460 --> 00:00:41,060
+موضوعنا عن الـ automatic pipette use and
+
+10
+00:00:41,060 --> 00:00:44,280
+calibration. خلينا نشوف إيش يعني automatic pipette
+
+11
+00:00:44,280 --> 00:00:47,480
+هلأ، احنا المحاضرة الماضية، خلينا بس نحكي هالنقطة.
+
+12
+00:00:47,790 --> 00:00:53,150
+المحاضرة الماضية كانت بتحكي عن الـ ... الـ ... الـ
+
+13
+00:00:53,150 --> 00:00:58,270
+volumes والحجوم والمحاليل، وكيف أنا بتعامل مع
+
+14
+00:00:58,270 --> 00:01:01,110
+المحاليل هذه، وإيش أنواع المحاليل اللي تستخدم في
+
+15
+00:01:01,110 --> 00:01:05,930
+الـ medical laboratories، و... وكيف أنا أُخفف هذه
+
+16
+00:01:05,930 --> 00:01:10,690
+المحاليل، تمام؟ طيب، لما أنا... أنا بدي أجي أنقل
+
+17
+00:01:10,690 --> 00:01:13,950
+المحلول من إلى، يعني مثلاً أنا عندي عينة بدي
+
+18
+00:01:13,950 --> 00:01:17,970
+أضيف لها reagent علشان أعمل فحص معين، أو بدي أأخذ
+
+19
+00:01:17,970 --> 00:01:22,130
+... بدي أنقل حجوم علشان أعمل dilution زي ما أخذنا
+
+20
+00:01:22,130 --> 00:01:25,800
+الـ... مسائل الـ dilution المحاضرة الماضية. كيف أنا
+
+21
+00:01:25,800 --> 00:01:29,140
+بدي أنقلها؟ هنا بالـ medical laboratories
+
+22
+00:01:29,140 --> 00:01:32,660
+بالمختبرات الطبية، احنا بنستخدم شيء اسمه automatic
+
+23
+00:01:32,660 --> 00:01:37,100
+pipette، اللي هو الأداة اللي موجودة بهذه الصورة. اسمها
+
+24
+00:01:37,100 --> 00:01:40,740
+automatic pipette. خلينا نشوف إيش يعني automatic pipette.
+
+25
+00:01:41,150 --> 00:01:45,250
+هلأ، automatic pipette هي عبارة عن أداة تستخدم في الـ
+
+26
+00:01:45,250 --> 00:01:48,110
+biological والـ biochemical والـ microbiological
+
+27
+00:01:48,110 --> 00:01:52,310
+labs، سواء كانت للـ teaching purposes أو للـ research
+
+28
+00:01:52,310 --> 00:01:56,490
+purposes، تمام؟ يعني تستخدم في مختبرات الأحياء، و
+
+29
+00:01:56,490 --> 00:01:59,750
+مختبرات الـ biochemistry والـ microbiology في كل
+
+30
+00:01:59,750 --> 00:02:04,700
+أقسام المختبرات الطبية، وتستخدم إما للتدريس، أو حتى
+
+31
+00:02:04,700 --> 00:02:09,640
+للعمل، لممارسة المهنة، أو لأغراض البحث العلمي. طبعاً
+
+32
+00:02:09,640 --> 00:02:13,700
+من شأن هذه الـ pipette أنها تعمل accurate transfer of
+
+33
+00:02:13,700 --> 00:02:18,500
+volume، يعني أنها تنقل الحجوم بشكل دقيق جداً. بمثاق
+
+34
+00:02:18,500 --> 00:02:22,050
+الكيمياء، يمكن أخذتم معامل كيمياء قبل هيك، كنتوا
+
+35
+00:02:22,050 --> 00:02:25,250
+تستخدموا pipettes اللي هي زي الـ glass pipette اللي كان
+
+36
+00:02:25,250 --> 00:02:29,490
+عبارة عن pipette زجاجية، وفي آخرها أو في
+
+37
+00:02:29,490 --> 00:02:33,210
+نهايتها بيكون في عندي مستودع من المطاط، أنا بتحكم
+
+38
+00:02:33,210 --> 00:02:37,160
+بدخول وخروج الهواء، ودخول وخروج السائل. إلى الـ pipette
+
+39
+00:02:37,160 --> 00:02:41,280
+طبعاً هذه الـ glass pipette بتستخدم بالـ chemical
+
+40
+00:02:41,280 --> 00:02:45,380
+laboratory، ولكن للأسف هي not accurate وnot
+
+41
+00:02:45,380 --> 00:02:49,820
+precise. مش accurate ومش precise متى؟ طبعاً لما بدي
+
+42
+00:02:49,820 --> 00:02:53,640
+أنقل حجوم أقل من one ml، واحنا هنا بالمختبرات
+
+43
+00:02:53,640 --> 00:02:57,000
+الطبية بنتعامل دائماً مع حجوم صغيرة جداً بالـ
+
+44
+00:02:57,000 --> 00:03:01,970
+microliter، يعني أقل من one ml. فبالتالي أنا بدي أداة
+
+45
+00:03:01,970 --> 00:03:07,230
+تنقل الحجم هذا الصغير، أو الحجم القليل، بطريقة more
+
+46
+00:03:07,230 --> 00:03:11,170
+accurate and more precise. how؟ باستخدام الـ
+
+47
+00:03:11,170 --> 00:03:15,470
+automatic pipette. إذا الـ glass pipette هي تستخدم بالـ
+
+48
+00:03:15,470 --> 00:03:19,670
+chemical laboratory، ولكن للأسف في الحجوم الصغيرة
+
+49
+00:03:19,670 --> 00:03:25,060
+not accurate، not precise. أنا محتاجة أداة تكون أكثر
+
+50
+00:03:25,060 --> 00:03:28,560
+دقة، more accurate، more precise. لهيك أنا بستخدم الـ
+
+51
+00:03:28,560 --> 00:03:32,200
+automatic pipette، لأن كل شغلي بيكون على الـ volumes
+
+52
+00:03:32,200 --> 00:03:37,480
+اللي أقل من one ml. طبعاً طيب، كل pipette من هدول الـ
+
+53
+00:03:37,480 --> 00:03:42,440
+automatic pipettes مصممة إن هي تنقل، يعني مدى أو
+
+54
+00:03:42,440 --> 00:03:47,050
+range من الحجوم، من إلى. يعني في pipette متكون مصممة
+
+55
+00:03:47,050 --> 00:03:50,930
+تنقل من واحد microliter لعشرة microliters، في من...
+
+56
+00:03:50,930 --> 00:03:54,770
+مصممة بتكون من عشرة microliters لمئة microliters، في من...
+
+57
+00:03:54,770 --> 00:03:58,610
+من مئة microliters لـ one ml. تمام؟ ف... وفي من one
+
+58
+00:03:58,610 --> 00:04:04,050
+ml لخمسة ml. فإذا الـ... في pipettes أنواع وحجوم، وكل
+
+59
+00:04:04,050 --> 00:04:08,390
+pipette بتكون لها range، يعني مدى من إلى.
+
+60
+00:04:08,390 --> 00:04:15,140
+بتستخدموا في الـ... الـ... الـ... نقل الحجوم هذه، و
+
+61
+00:04:15,140 --> 00:04:19,600
+طبعاً احنا المفروض أن احنا ما نتخطى هذا الـ... الـ... الـ
+
+62
+00:04:19,600 --> 00:04:23,820
+range تبعها. يعني لازم أنا مثلاً، إذا بدي أنقل خمسين
+
+63
+00:04:23,820 --> 00:04:28,320
+microliter، يفضل أن أنا آخذ pipette الـ range تبعها
+
+64
+00:04:28,320 --> 00:04:31,480
+بحيث أن الخمسين تكون واقعة في الـ range، في داخل
+
+65
+00:04:31,480 --> 00:04:34,980
+هذا الـ range. يعني مثلاً، بأخذ pipette بتنقل من عشرة
+
+66
+00:04:34,980 --> 00:04:38,420
+microliters لمئة microliters، الـ volume رقم خمسين
+
+67
+00:04:38,420 --> 00:04:42,580
+هيكون already موجود، يعني في المدى، والـ pipette بتقدر
+
+68
+00:04:42,580 --> 00:04:46,880
+أنها تنقل لي هذا الـ volume. فبالتالي أنا هكون ضمنت الـ
+
+69
+00:04:46,880 --> 00:04:50,250
+accuracy والـ precision لنقل الـ volumes. طيب، إذا
+
+70
+00:04:50,250 --> 00:04:54,850
+إحنا نوّهنا إنه أنا في عندي أنواع وحجوم، ويعني قدرة
+
+71
+00:04:54,850 --> 00:04:58,930
+استيعابية للـ pipettes، يعني مش كلها واحدة، مش كلها
+
+72
+00:04:58,930 --> 00:05:03,930
+مصممة إنها تنقل لي نفس الحجم. طيب، فإذا في عندي حاجة
+
+73
+00:05:03,930 --> 00:05:08,010
+اسمها pipette man model. طبعاً هي سبعة أنواع من الـ
+
+74
+00:05:08,010 --> 00:05:12,440
+pipettes، مصممة إنها تنقل حجوم بطريقة مختلفة. طبعاً
+
+75
+00:05:12,440 --> 00:05:17,560
+هذه الـ... الـ man model، يعني لو هم السبعة بيكونوا
+
+76
+00:05:17,560 --> 00:05:20,980
+بينقلوا لي من 0.1 microliter، يعني من أقل من
+
+77
+00:05:20,980 --> 00:05:24,680
+واحد microliter لحد خمسة آلاف microliter، يعني لحد
+
+78
+00:05:24,680 --> 00:05:30,100
+خمسة ml. يعني في تنوع من الـ pipettes هذه، وهلأ بنشوف
+
+79
+00:05:30,100 --> 00:05:35,090
+بالجدول اللي في الـ slide التالية، هيوضح لي إيش
+
+80
+00:05:35,090 --> 00:05:38,970
+يعني pipette مع الموديل. ولكن هنا بيحكي لنا إنه the
+
+81
+00:05:38,970 --> 00:05:42,710
+most often used، يعني الأكثر استخداماً هي الـ pipettes
+
+82
+00:05:42,710 --> 00:05:49,150
+اللي تسمى الـ B20 والـ B200 والـ B1000. هدول
+
+83
+00:05:49,150 --> 00:05:54,150
+الـ models هم الأكثر استخداماً، اللي بينقلوا لي حجم من
+
+84
+00:05:54,150 --> 00:05:58,450
+اثنين لعشرين، ومن خمسين لمائتين، ومن مئة لألف
+
+85
+00:05:58,450 --> 00:06:02,170
+microliter. تمام؟ هذه المدى تبعهم respectively، مرتب
+
+86
+00:06:02,170 --> 00:06:07,410
+بالترتيب. الـ B20 بتنقل من اثنين لعشرين microliter.
+
+87
+00:06:07,410 --> 00:06:12,990
+تمام؟ والـ pipette B200 بتنقل volume من خمسين لمائتين
+
+88
+00:06:12,990 --> 00:06:16,730
+microliter، والـ pipette B1000 بتأخذ من مئة لألف
+
+89
+00:06:16,730 --> 00:06:20,970
+microliter. يعني الرقم اللي جنب الـ B، تمام؟ يعني
+
+90
+00:06:20,970 --> 00:06:25,240
+pipette، والرقم هذا يعني أقصى قيمة بتقدر تأخذها. طبعاً
+
+91
+00:06:25,240 --> 00:06:28,800
+ومش... مش أدنى قيمة، لأ، في أدنى... الأدنى قيمة غير
+
+92
+00:06:28,800 --> 00:06:32,080
+مذكورة. هلأ نيجي نشوف الـ pipette man model، هي في
+
+93
+00:06:32,080 --> 00:06:36,960
+عندي واحد، اثنين، أربعة، ستة، سبعة أنواع من الـ pipette
+
+94
+00:06:36,960 --> 00:06:43,870
+طبعاً، ومسمى بـ B2، يعني بتنقل من صفر للاثنين. وطبعاً الـ
+
+95
+00:06:43,870 --> 00:06:51,010
+B20 من صفر لعشرة، بتأخذ الـ B20 بتأخذ من صفر لعشرين، الـ B100
+
+96
+00:06:51,010 --> 00:06:55,250
+بتأخذ من صفر لمئة، و200 من صفر لمائتين، وهكذا، من صفر لـ
+
+97
+00:06:55,250 --> 00:07:00,570
+1000، يعني من ألف. وأما هنا من صفر لخمسة آلاف، لحد one...
+
+98
+00:07:00,570 --> 00:07:04,310
+لحد خمسة ml. هذا الـ range، بصي باللي بيكون
+
+99
+00:07:04,310 --> 00:07:07,690
+مسجل على الـ pipette نفسها. يعني بيكون... بيكون...
+
+100
+00:07:07,690 --> 00:07:12,960
+بيعطيني الـ range إنه من... من كذا لكذا. أنا لما بدي
+
+101
+00:07:12,960 --> 00:07:16,040
+أستخدم الـ pipette هذه، طبعاً أنا ما بستخدم الـ pipette
+
+102
+00:07:16,040 --> 00:07:18,700
+عشان أنقل zero volume. يعني أنا ما بديش أنقل volume
+
+103
+00:07:18,700 --> 00:07:21,840
+ليش أستخدم الـ pipette؟ zero volume، يعني no volume.
+
+104
+00:07:21,840 --> 00:07:26,160
+طبعاً، فإذا في عندي حاجة اسمها recommended range.
+
+105
+00:07:26,160 --> 00:07:29,600
+يعني الـ range الموصى به. يعني أنا بستخدم الـ pipette
+
+106
+00:07:29,600 --> 00:07:36,260
+لحتى إنه أنا، يعني في range مسموح لي فيه. إيش هو اللي
+
+107
+00:07:36,260 --> 00:07:42,040
+مسموح لي فيه؟ من 0.1 للاثنين. وطبعاً الـ... و
+
+108
+00:07:42,040 --> 00:07:45,880
+هنا من نصف لعشرة، من اثنين لعشرين، من عشرة لمئة، و
+
+109
+00:07:45,880 --> 00:07:50,400
+هكذا، من خمسين لمائتين. طيب، هذه الـ pipette اللي من
+
+110
+00:07:50,400 --> 00:07:53,780
+خمسين لمائتين، ممكن تسحب أقل من خمسين؟ اه، ممكن تسحب
+
+111
+00:07:53,780 --> 00:07:56,560
+أقل من خمسين. الـ range تبعها أصلاً من صفر لمائتين.
+
+112
+00:07:56,900 --> 00:08:00,800
+ولكن هو إذا سحبت أقل من خمسين، فهي بتكون inaccurate
+
+113
+00:08:00,800 --> 00:08:05,200
+and imprecise. يعني مش accurate ومش precise. فأنا
+
+114
+00:08:05,200 --> 00:08:08,300
+يفضل أن أنا أستخ... أخت... يعني أتبع الـ
+
+115
+00:08:08,300 --> 00:08:13,460
+recommended range، علشان أستخ... أختار الـ... الـ...
+
+116
+00:08:13,460 --> 00:08:18,170
+الـ pipette المناسبة لي، تمام؟ طيب، يعني مثلاً لو أنا بدي
+
+117
+00:08:18,170 --> 00:08:23,030
+أنقل خمسين microliter، تمام؟ خمسين microliter، مين
+
+118
+00:08:23,030 --> 00:08:29,190
+أفضل أختار الـ... الـ pipette مثلاً رقم الـ B100 ولا الـ
+
+119
+00:08:29,190 --> 00:08:33,410
+B200؟ يفضل أن أنا أختار الـ B100، لإنه الخمسين
+
+120
+00:08:33,410 --> 00:08:36,570
+القيمة خمسين، تقع في منتصف الـ range. يعني أنا ب... بكون
+
+121
+00:08:36,570 --> 00:08:41,710
+ضمن إنه ما فيش أخطاء. تمام؟ الـ pipette على الـ border،
+
+122
+00:08:41,710 --> 00:08:45,850
+على الحدود، تمام؟ ممكن يكون فيها نسبة خطأ، لكن في
+
+123
+00:08:45,850 --> 00:08:49,950
+منتصف الـ range بتكون دقيقة. فإذا أنا بفضل أن أنا
+
+124
+00:08:49,950 --> 00:08:54,810
+لما بدي أختار اللي بدي أنقل volume، بختار الـ pipette
+
+125
+00:08:54,810 --> 00:08:57,870
+اللي هذا الـ volume يتوسط الـ range تبعها، أو موجود
+
+126
+00:08:57,870 --> 00:09:02,120
+في الـ range تبعها. طبعاً، طيب، هلأ لو بدي أنقل مثلاً مئة
+
+127
+00:09:02,120 --> 00:09:08,300
+microliter، مين أفضل أختار؟ الـ B100 طبعاً، ولا الـ B200
+
+128
+00:09:08,300 --> 00:09:15,620
+ولا الـ B1000؟ يفضل أن أنا أختار الـ B200، طبعاً. وممكن
+
+129
+00:09:15,620 --> 00:09:18,560
+أنا أختار الـ B100، بتكون دقيقة. يعني الـ higher range
+
+130
+00:09:18,560 --> 00:09:23,040
+تبعها بتكون أدق دائماً. طبعاً الـ higher range بتكون
+
+131
+00:09:23,040 --> 00:09:26,680
+أدق من الـ lower range للـ pipette. طبعاً يعني هذه الـ
+
+132
+00:09:26,680 --> 00:09:32,120
+pipette الـ B100، يعني لو بدي أنقل 100 microliter من الـ
+
+133
+00:09:32,120 --> 00:09:37,620
+volume، ممكن أستخدم الـ B200، الـ... الـ... الـ B100.
+
+134
+00:09:37,620 --> 00:09:43,440
+ممكن أستخدم الـ B1000. تمام؟ و... لكن مثلاً هذه الـ pipette
+
+135
+00:09:43,440 --> 00:09:47,500
+اللي هو الـ range تبعها من عشرة لمئة. تمام؟ عند
+
+136
+00:09:47,500 --> 00:09:50,800
+المئة بتكون أدق من من... من... من أني أسحب فيها
+
+137
+00:09:50,800 --> 00:09:54,640
+عشرة microliters. يعني دائماً الـ higher limit تبعها
+
+138
+00:09:54,640 --> 00:09:57,990
+بتكون أدق من الـ lower limit للـ pipette نفسها. طبعاً
+
+139
+00:09:57,990 --> 00:10:02,750
+إذا نرجع نعيد الصياغة ثانيةً، لو أنا في عندي... بدي
+
+140
+00:10:02,750 --> 00:10:05,690
+أنقل 100 microliter، مين أفضل أستخدم؟ الأفضل أن أنا
+
+141
+00:10:05,690 --> 00:10:09,990
+أستخدم الـ B200، لأن المئة تتوسط الـ range. ممكن
+
+142
+00:10:09,990 --> 00:10:13,730
+أستخدم الـ B100، لإنه حكينا الـ higher limit بتكون
+
+143
+00:10:13,730 --> 00:10:16,990
+أدق دائماً من الـ lower limit للـ pipette، ولكن أنا
+
+144
+00:10:16,990 --> 00:10:20,110
+ما بستخدم... يعني ممكن أستخدمها، بس ما بكون دقيقة، و
+
+145
+00:10:20,110 --> 00:10:24,170
+... و... كثيراً. لإنه دائماً حكينا إنه الـ lower limit
+
+146
+00:10:24,170 --> 00:10:27,370
+للـ pipette بتكون أقل دقة من الـ higher limit. هذا
+
+147
+00:10:27,370 --> 00:10:31,690
+بالنسبة لـ... الـ... الـ... الـ pipette man model، حكينا
+
+148
+00:10:31,690 --> 00:10:36,770
+مين هم أكثر شيوعاً وأكثر استخداماً؟ B20 والـ
+
+149
+00:10:36,770 --> 00:10:41,910
+B100 والـ B200، تمام؟ إذا الـ B20، الـ B100 والـ B200 هم أكثر الـ... الـ pipettes
+
+150
+00:10:41,910 --> 00:10:46,570
+شيوعاً واستخداماً. تمام؟ طيب، هلأ بدنا نحكي عن
+
+151
+00:10:46,570 --> 00:10:50,570
+أنواع... يعني، sorry، مكونات الـ pipette وأجزاؤها. طيب،
+
+152
+00:10:50,570 --> 00:10:56,150
+الـ pipette، زي ما أنتم شايفين بهذا الشكل، مكونة من حاجة
+
+153
+00:11:00,430 --> 00:11:05,030
+اسمها الـ... البش... البشطون أو البلنجر، نحكي عنه
+
+154
+00:11:05,030 --> 00:11:09,770
+إما بشطون أو بلنجر. إيش يعني بشطون أو بلنجر؟ زي ما
+
+155
+00:11:09,770 --> 00:11:13,250
+أنتم شايفين، هذا الـ push button، هو عبارة عن كبسة
+
+156
+00:11:13,250 --> 00:11:18,830
+push، أنا ممكن أنا أضغط عليها، وتطلع وتنزل من الـ
+
+157
+00:11:18,830 --> 00:11:24,030
+pipette button. تمام؟ وهذا الـ rod تبعها. طبعاً هذا الـ rod الـ
+
+158
+00:11:24,030 --> 00:11:29,980
+... الـ... الـ... الـ... القضيب هذا، ممكن إنه يلتف دائرياً
+
+159
+00:11:29,980 --> 00:11:35,500
+علشان يتحكم في الـ volume. تمام؟ يتحكم في الـ volume
+
+160
+00:11:35,500 --> 00:11:39,620
+اللي موجودة على الـ digital screen هنا. تمام؟ هلأ في
+
+161
+00:11:39,620 --> 00:11:45,240
+هنا adapter موجود. هذا يعني علشان أنا أُقلل من حركة
+
+162
+00:11:45,240 --> 00:11:47,800
+الـ pipette. يعني ممكن أنا أمسك هذا الـ adapter على
+
+163
+00:11:47,800 --> 00:11:51,880
+إصبعي... على إصبعي السبابة، وأمسكها بشكل إنه يكون
+
+164
+00:11:51,880 --> 00:11:58,230
+أنا متحكمة فيها، وأمسكها بشكل صحيح. هذا بسميه tip
+
+165
+00:11:58,230 --> 00:12:04,410
+ejector... tip ejector أو tip eject button. تمام؟ هو
+
+166
+00:12:04,410 --> 00:12:09,730
+عبارة عن كبسة بتتخلص من الـ tip،
+
+223
+00:16:29,140 --> 00:16:32,720
+بالـ bybit في المعامل أنا بأخذ الـ bybit .. بأخذ الطالبة
+
+224
+00:16:32,720 --> 00:16:37,120
+تمسك الـ bybit تمام، وبحيث أن الـ digital screen
+
+225
+00:16:37,120 --> 00:16:41,300
+تكون مواجهة لها تمام، يعني تكون عينها على الـ
+
+226
+00:16:41,300 --> 00:16:45,800
+digital screen وبالتالي بما أنه بيكون عينها على الـ
+
+227
+00:16:45,800 --> 00:16:49,660
+digital screen، إذا حركت هذا الـ bybit بشكل دائري
+
+228
+00:16:49,660 --> 00:16:54,260
+باتجاهها، يعني لعندها تمام، هيك بيزيد الـ volume، أما
+
+229
+00:16:54,260 --> 00:16:59,180
+إذا حركته باتجاه المعاكس، يعني بعيد عنها، فهو بيقل
+
+230
+00:16:59,180 --> 00:17:02,340
+الـ volume، تمام؟ هذا بده ممارسة، إن شاء الله بنشرحه
+
+231
+00:17:02,340 --> 00:17:09,020
+بالطريقة أو بالتجربة العملية، تمام؟ هذا بالنسبة لـ ..
+
+232
+00:17:09,020 --> 00:17:14,320
+الـ .. الـ .. الـ bybit  والطرق أو اللي هي الخطوات
+
+233
+00:17:14,320 --> 00:17:17,740
+أو اللي .. الـ .. التعليمات اللي لازم أنا ألتزم
+
+234
+00:17:17,740 --> 00:17:22,920
+فيها أثناء السحب أو نقل الحجوم باستخدام الـ bybit
+
+235
+00:17:23,850 --> 00:17:29,330
+في شغل هنا، إن الـ .. الـ bybit هنا طبعاً هي بتكون
+
+236
+00:17:29,330 --> 00:17:33,210
+مصممة إنها تنقلني أو تقفز قفزات في الـ volume، يعني
+
+237
+00:17:33,210 --> 00:17:38,970
+مثلاً هاي هنا بحكي لي إن هي صح يعني من خمسين لـ مئة
+
+238
+00:17:38,970 --> 00:17:45,210
+بتسحب، لكن إن مثلاً أنا لما بدي أحرك بصير أحرك
+
+239
+00:17:45,210 --> 00:17:48,450
+بيزيد الـ volume كل شوية point اثنين، point اثنين
+
+240
+00:17:48,450 --> 00:17:51,890
+لحد ما أوصل للـ volume اللي بدي إياها، لذا هذه هي الـ ..
+
+241
+00:17:51,890 --> 00:17:55,450
+الـ .. مدى التحريك أو اللي هي القفزات في الـ
+
+242
+00:17:55,450 --> 00:18:01,330
+volumes، طبعاً طيب، place a tip on the
+
+243
+00:18:01,330 --> 00:18:05,230
+discharge end of the pipet، طبعاً أنا بـ .. بركب الـ
+
+244
+00:18:05,230 --> 00:18:10,370
+tip في المكان المخصص لها، وبعد هيك طبعاً بـ ..
+
+245
+00:18:12,600 --> 00:18:16,600
+باستخدام البلانجر بـ .. باستخدمه عشان أسحب الـ
+
+246
+00:18:16,600 --> 00:18:20,520
+volumes، هذا البلانجر اللي إحنا شايفينه، تمام؟ مش ما
+
+247
+00:18:20,520 --> 00:18:24,180
+.. أيضاً هو يعني فايد .. فائدته مش بس إنه متصل
+
+248
+00:18:24,180 --> 00:18:28,360
+بالـ bybit  و بتحكم بالـ volume، لأ هذا
+
+249
+00:18:28,360 --> 00:18:31,600
+البلانجر إحنا بنسميه push button، يعني أنا ممكن أكبس
+
+250
+00:18:31,600 --> 00:18:35,480
+عليه بيطلع وبينزل، طبعاً أنا هذا لما بدي أكبس عليه و
+
+251
+00:18:35,480 --> 00:18:39,860
+يطلع وينزل، بينزل عند ضغطتين، الضغطة الأولى لما
+
+252
+00:18:39,860 --> 00:18:43,840
+أضغط الضغطة الأولى بيقل لي الـ exact volume اللي
+
+253
+00:18:43,840 --> 00:18:50,720
+موجود ع الشاشة، تمام؟ لما أضغط ضغطتين، تمام؟ بينقل لي
+
+254
+00:18:50,720 --> 00:18:54,280
+extra volume، ممكن يكون حجم مضاعف أو extra volume
+
+255
+00:18:54,280 --> 00:18:59,560
+من الـ volume اللي مصمم أو موجود على الشاشة، تمام؟
+
+256
+00:18:59,560 --> 00:19:04,400
+إذا هذا الـ push، هذا البلانجر عبارة عن كبسة فيها two
+
+257
+00:19:04,400 --> 00:19:07,680
+stops، يعني أنا لما أضغط أول ضغطة بلاغظ إنه خلاص
+
+258
+00:19:07,680 --> 00:19:12,780
+وصل إصبعي لدرجة معينة وأنا بضغط، لو ضغطت بقوة أكبر
+
+259
+00:19:12,780 --> 00:19:16,910
+ممكن أضغط الضغطة الثانية، يعني بنزل كمان مرة، فالضغطة
+
+260
+00:19:16,910 --> 00:19:21,410
+الأولى لما أنا بكون ضاغطة إياها بتكون الـ bybit بتسحب لي
+
+261
+00:19:21,410 --> 00:19:25,750
+volume نفس الـ volume اللي موجودة ع الشاشة، لكن لو
+
+262
+00:19:25,750 --> 00:19:29,690
+أنا بدي أسحب two volumes أو يعني بصراحة بدي أسحب و
+
+263
+00:19:29,690 --> 00:19:33,110
+أنا ضغطت ضغطتين، هيسحب لي الـ volume اللي موجودة ع
+
+264
+00:19:33,110 --> 00:19:37,610
+الشاشة وكمان extra volume ثاني، يعني volume زيادة
+
+265
+00:19:37,610 --> 00:19:40,710
+طيب أنا ليش؟ إمتى بستخدم الضغطة الأولى وإمتى بستخدم
+
+266
+00:19:40,710 --> 00:19:45,100
+الضغطة الثانية أو الضغطتين؟ هلأ بنشوف، هي هنا
+
+267
+00:19:45,100 --> 00:19:47,920
+بيحكي لي إن البلانجر أو اللي هو الـ push button
+
+268
+00:19:47,920 --> 00:19:53,140
+تمام، و الـ stops at two different positions، يعني هذا
+
+269
+00:19:53,140 --> 00:19:57,740
+البلانجر بيتوقف عند .. عند مكانين، وأنا لما أجي
+
+270
+00:19:57,740 --> 00:20:00,820
+أضغط عليه بلاغظ إنه في عندي two resistance، في
+
+271
+00:20:00,820 --> 00:20:05,020
+مقاومتين للضغطة تبعي، الـ .. الـ stop الأول أو
+
+272
+00:20:05,020 --> 00:20:10,160
+الضغطة الأولى، تمام؟ ولو أنا زودت الـ .. الضغط تبعي
+
+273
+00:20:10,160 --> 00:20:16,630
+بنزل كمان ضغطة ثانية، فبالتالي الـ .. الـ .. الـ first
+
+274
+00:20:16,630 --> 00:20:21,870
+stopping، تمام؟ عند الـ initial resistance، تمام؟ عند
+
+275
+00:20:21,870 --> 00:20:27,610
+أول يعني stop، عند أول إعاقة لضغطة تبعي بيقف الـ ..
+
+276
+00:20:27,610 --> 00:20:30,850
+الـ plunger عندها، بتكون هذه عبارة عن الضغطة الأولى
+
+277
+00:20:31,230 --> 00:20:37,590
+طبعاً، و الـ .. و هذه معناها إن أنا هسحب إيش؟ الـ
+
+278
+00:20:37,590 --> 00:20:40,810
+desired volume، يعني أنا هاخد الـ volume اللي موجود
+
+279
+00:20:40,810 --> 00:20:47,720
+ع الشاشة exactly، باخذه، طبعاً الـ second stopping لما
+
+280
+00:20:47,720 --> 00:20:53,380
+يكون .. لما أنا بدي أضغط على الضغط الثاني، يعني أنا
+
+281
+00:20:53,380 --> 00:20:58,900
+بدي أظل نازلة في البلانجر ضغطة، ضغطتين، فساعتها أنا بكون
+
+282
+00:20:58,900 --> 00:21:02,540
+تجاوزت الـ initial resistance، عند .. تجاوزت الإعاقة
+
+283
+00:21:02,540 --> 00:21:06,540
+الأولى وإنزلت كمان مرة في الـ .. في البلانجر، طبعاً
+
+284
+00:21:06,540 --> 00:21:12,600
+فهذا الشيء معناها إن الـ bybit راح تسحب لي
+
+285
+00:21:12,600 --> 00:21:17,940
+volume extra، طبعاً هتسحب لي الـ .. الـ volume extra
+
+286
+00:21:17,940 --> 00:21:22,720
+ساعتها أنا لما بدي أفرغ الحجم، بفرغه بضغطة واحدة
+
+287
+00:21:22,950 --> 00:21:26,350
+هينقل الحجم، وهيضل في عندي extra volume موجود في
+
+288
+00:21:26,350 --> 00:21:31,130
+الـ tip، ممكن أتخلص منه، تمام؟ إذا في عندي الـ .. الـ
+
+289
+00:21:31,130 --> 00:21:34,370
+.. بلانجر الـ .. أو الـ .. الـ push button بيقف عند
+
+290
+00:21:34,370 --> 00:21:38,430
+ضغطة، ضغطتين، الضغطة الأولى بتنـ .. لما أنا أكون ضاغطة ها و
+
+291
+00:21:38,430 --> 00:21:42,270
+أسحب حجم من السائل، معناها أنا سحبت نفس الحجم اللي
+
+292
+00:21:42,270 --> 00:21:45,600
+موجودة على الشاشة، لكن إذا أنا كنت ضغطة، ضغطة، ضغطتين و
+
+293
+00:21:45,600 --> 00:21:50,120
+سحبت حجم، معناها أنا سحبت الحجم مع extra، تمام؟ لما
+
+294
+00:21:50,120 --> 00:21:54,040
+أجي أفرغه، بدي انتبه إن أنا أضغط ضغطة واحدة فقط
+
+295
+00:21:54,040 --> 00:21:57,520
+أفرغ الـ volume اللي من ضغطة واحدة، لأن ضغطة واحدة
+
+296
+00:21:57,520 --> 00:22:00,380
+يعني إيش؟ الـ desired volume، يعني الـ volume اللي
+
+297
+00:22:00,380 --> 00:22:04,580
+بدي إياها، وبيضل في عندي extra، طبعاً، طيب ليش الغلبة هذه؟
+
+298
+00:22:04,580 --> 00:22:07,880
+ليش ضغطة واحدة وضغطتين، وليش أنا آخد extra volume؟
+
+299
+00:22:08,030 --> 00:22:11,550
+عشان في عندي نوعين من الـ bypipetting، نوع اسمه
+
+300
+00:22:11,550 --> 00:22:16,450
+forward pipetting و reverse pipetting، إيش يعني
+
+301
+00:22:16,450 --> 00:22:19,770
+forward pipetting؟ يعني إن أنا أفوت في المحلول
+
+302
+00:22:19,770 --> 00:22:23,870
+تمام، وأنا ضغطة، ضغطة واحدة، آخد الحجم اللي بدي إياه، هو
+
+303
+00:22:23,870 --> 00:22:27,390
+نفس الحجم اللي موجود على الشاشة exactly، بالضبط
+
+304
+00:22:27,390 --> 00:22:32,530
+بدون زيادة، وبعد هيك أجي أفرغه، أفرغه في المحلول، في
+
+305
+00:22:32,530 --> 00:22:37,350
+الوعاء اللي أنا بدي أشتغل فيه، وبفرغه، وبضل ضغطة
+
+306
+00:22:37,540 --> 00:22:40,020
+ممكن أضغط أكثر من مرة، أو ممكن أضغط أكثر من مرة، أو
+
+307
+00:22:40,020 --> 00:22:44,880
+ممكن أضغط أكثر من مرة، أو ممكن أضغط أكثر من مرة، أو
+
+308
+00:22:44,880 --> 00:22:48,820
+ممكن أضغط أكثر من مرة، أو ممكن أضغط أكثر من مرة، أو
+
+309
+00:22:48,820 --> 00:22:48,960
+ممكن أضغط أكثر من مرة، أو ممكن أضغط أكثر من مرة، أو
+
+310
+00:22:48,960 --> 00:22:49,180
+ممكن أضغط أكثر من مرة، أو ممكن أضغط أكثر من مرة، أو
+
+311
+00:22:49,180 --> 00:22:49,860
+ممكن أضغط أكثر من مرة، أو ممكن أضغط أكثر من مرة، أو
+
+312
+00:22:49,860 --> 00:22:52,000
+ممكن أضغط أكثر من مرة، أو ممكن أضغط أكثر من مرة، أو
+
+313
+00:22:52,000 --> 00:22:53,780
+ممكن أضغط أكثر من مرة، إذا إيش الـ forward
+
+314
+00:22:53,780 --> 00:22:57,380
+pipetting؟ معناها .. معناها إن أنا أضغط ضغطة على الـ
+
+315
+00:22:57,380 --> 00:23:01,680
+pipet بعدين أجي أسـ .. أـ .. أنقل الحجم، آخد الحجم
+
+316
+00:23:01,680 --> 00:23:04,200
+اللي بدي إياه، هو الـ exact volume اللي موجود على
+
+317
+00:23:04,200 --> 00:23:08,120
+الشاشة، ولما أجي أفرغه في الوعاء ممكن أضغط ضغط أو
+
+318
+00:23:08,120 --> 00:23:11,040
+ضغطتين عشان أتأكد إن أنا فرغته كله وإن أنا
+
+319
+00:23:11,040 --> 00:23:14,700
+استخدمت الـ volume بالضبط هو نفس اللي موجود على
+
+320
+00:23:14,700 --> 00:23:17,720
+الشاشة، تمام؟ ما يكون فيه ضاـ .. إيش؟ يضلّ شيء عالق في الـ
+
+321
+00:23:17,720 --> 00:23:23,070
+tip، تمام؟ إذا .. هذا معناه الـ forward، وهذه الطريقة
+
+322
+00:23:23,070 --> 00:23:27,410
+تستخدم عشان أنا لو بدي أنقل حجوم زي مين؟ زي الـ
+
+323
+00:23:27,410 --> 00:23:31,450
+aqueous solution، المحاليل اللي أصلها ماء، الـ buffers،
+
+324
+00:23:31,450 --> 00:23:35,310
+الـ acids، الـ bases، كل الأشياء، المحاليل هذه اللي أصلها
+
+325
+00:23:35,310 --> 00:23:39,750
+ماء، بستخدم معاها طريقة الـ forward pipetting، أما
+
+326
+00:23:39,750 --> 00:23:43,390
+طريقة الـ reverse pipetting فهي عبارة عن إن أنا
+
+327
+00:23:43,390 --> 00:23:47,990
+بأخذ larger volume of solution، بسحب larger volume
+
+328
+00:23:47,990 --> 00:23:51,990
+of solution، إيش يعني؟ يعني بسحب extra، يعني معناها
+
+329
+00:23:51,990 --> 00:23:55,430
+أنا لما باجي باخد الـ bypipet أو بنقل في الـ pipet
+
+330
+00:23:55,430 --> 00:24:00,870
+بكون ضغطة، ضغطتين، تمام؟ باخد الحجم اللي بدي إياه مع
+
+331
+00:24:00,870 --> 00:24:05,750
+extra، تمام؟ ولما أجي أفرغ في المحلول بفرغ بواحدة
+
+332
+00:24:05,750 --> 00:24:11,030
+بحيث إن أنا أفرغ فقط المحلول، الحجم اللي بدي إياه، ويضل
+
+333
+00:24:11,030 --> 00:24:14,330
+الـ extra اللي موجود في الـ tip أتخلص منه، طيب أنا ليش
+
+334
+00:24:14,330 --> 00:24:20,430
+يعني بلجأ لهذه الطريقة؟ بيحكي لي لإنه في سوائل عبارة
+
+335
+00:24:20,430 --> 00:24:25,250
+عن لازجة، viscous، طبعاً هذا إيش يعني؟ viscous لازجة
+
+336
+00:24:25,250 --> 00:24:29,130
+يعني بصعوبة هتطلع جوة الـ tip، المحلول بصعوبة هتطلع
+
+337
+00:24:29,130 --> 00:24:35,520
+جوة الـ tip، طبعاً طيب، فبالتالي لما أجي أنا أكون أخذة
+
+338
+00:24:35,520 --> 00:24:39,200
+الـ volume وزيادة شوية، هكون ضامنة إن أنا أخدت الـ
+
+339
+00:24:39,200 --> 00:24:42,060
+volume، يعني ضامنة إن أنا هنزل الـ volume على
+
+340
+00:24:42,060 --> 00:24:47,480
+الأقل اللي بدي إياه، تمام؟ لكن مثلاً لو أنا  استخدمت
+
+341
+00:24:47,480 --> 00:24:52,430
+مع هذه المحاليل الـ viscous، استخدمت نظام ضغطة واحدة،
+
+342
+00:24:52,430 --> 00:24:57,050
+ما هي بتطلع جوة الـ tip ببطء، يعني أنا ممكن أفوت وأطلع
+
+343
+00:24:57,050 --> 00:25:01,590
+وآخد الـ باقي الحجم اللي بدي إياه، بس لسه السائل من
+
+344
+00:25:01,590 --> 00:25:08,200
+كتر لزوجته ما بيطلع جوة الـ tip بشكل كامل، فبالتالي ممكن
+
+345
+00:25:08,200 --> 00:25:12,800
+آخد volume أقل من اللي أنا بدي إياه، فهنا ممكن يكون في
+
+346
+00:25:12,800 --> 00:25:17,120
+خطأ، عشان هيك بيحكي لي إن استخدمي مع السوائل اللازجة
+
+347
+00:25:17,120 --> 00:25:21,200
+إن طريقة إنك أنت تاخدي زيادة، ولما تفضي تفضي
+
+348
+00:25:21,200 --> 00:25:27,230
+الحجم اللي بدك إياه، تمام؟ هذا بالنسبة لمين؟ لـ .. الـ ..
+
+349
+00:25:27,230 --> 00:25:31,150
+الـ .. السوائل اللازجة، بستخدم معاها الـ reverse
+
+350
+00:25:31,150 --> 00:25:34,330
+technique، يعني نظام الضغطتين عشان آخد extra volume
+
+351
+00:25:34,330 --> 00:25:37,590
+أيضاً بيحكي لي فيه محاليل volatile، إيش يعني
+
+352
+00:25:37,590 --> 00:25:42,030
+volatile؟ يعني متطايرة، يعني مجرد مثلاً ما أسحبها في
+
+353
+00:25:42,030 --> 00:25:46,350
+الـ pipet وأنقلها للـ .. للوعاء عشان أفضيها، ممكن
+
+354
+00:25:46,350 --> 00:25:50,810
+تكون تبخرت، فهنا بيصير في عندي فاقد في الحجم
+
+355
+00:25:50,810 --> 00:25:58,290
+فبالتالي أنا بكون نقلت حجم أقل من اللازم، فهنا بـأستخدم
+
+356
+00:25:58,290 --> 00:26:05,490
+مع هذا النوع من السوائل أيضاً ممكن إن أنا أستخدم نظام
+
+357
+00:26:05,490 --> 00:26:09,230
+الضغطتين بحيث إن أنا آخد حجم زيادة، ولما أفضي أفضي
+
+358
+00:26:09,230 --> 00:26:14,030
+exact volume، عشان إذا مهما كان اللي طار من هذا الـ
+
+359
+00:26:14,030 --> 00:26:18,050
+solution، أكون على الأقل مازلت محافظة على الـ volume
+
+360
+00:26:18,050 --> 00:26:21,890
+اللي أنا بدي إياه، تمام؟ أيضاً في بعض المحاليل اللي
+
+361
+00:26:21,890 --> 00:26:24,510
+بنسميها foaming solution، إيش يعني foaming
+
+362
+00:26:24,510 --> 00:26:28,950
+solution؟ يعني محاليل بتعمل رغوة، تمام؟ فيها مادة
+
+363
+00:26:28,950 --> 00:26:33,190
+صابون أو يعني مادة .. لها ملمس الصابون، هذه
+
+364
+00:26:33,190 --> 00:26:37,690
+المحاليل اللي فيها مواد ذات ملمس الصابون، تمام؟
+
+365
+00:26:37,690 --> 00:26:41,120
+ممكن تعمل رغوة، وبالتالي لما أجي أسحب أسحب من
+
+366
+00:26:41,120 --> 00:26:45,220
+الرغوة، فبالتالي أكون نقلت volume أقل من
+
+367
+00:26:45,220 --> 00:26:49,520
+اللازم، لأن أنا يعني جزء من الرغوة احتلّ الـ tip
+
+368
+00:26:49,520 --> 00:26:53,300
+تمام؟ وأفكر حالي إن أنا أخدت الحجم اللي بدي إياه و
+
+369
+00:26:53,300 --> 00:26:59,700
+فعلياً أنا بكون أخذة حجم أقل من الـ .. المفروض
+
+370
+00:26:59,700 --> 00:27:03,300
+فبالتالي بستخدم برضه نظام الـ larger volume of
+
+371
+00:27:03,300 --> 00:27:06,120
+solution، يعني إن أنا بدي آخد الـ extra volume، يعني
+
+372
+00:27:06,120 --> 00:27:09,700
+بدي أستخدم الـ reverse technique، يعني نظام ضغطتين و
+
+373
+00:27:09,700 --> 00:27:17,300
+.. وبعد هيك إيش اسمه؟ بروح بفضي بضغطة واحدة، تمام؟ عشان
+
+374
+00:27:17,300 --> 00:27:21,780
+لو كان في رغوة في المحلول أكون ضامنة إنها ما تأثّر
+
+375
+00:27:21,780 --> 00:27:25,360
+على الحجم اللي أنا بدي أنقله، تمام؟ وأيضاً عشان لو
+
+376
+00:27:25,360 --> 00:27:29,180
+أنا بدي أنقل حجوم صغيرة جداً جداً، يعني أنا خائفة ..
+
+377
+00:27:29,180 --> 00:27:32,260
+يعني مش متأكدة أخدت الـ volume ولا لأ، لأنه في حجوم
+
+378
+00:27:32,260 --> 00:27:35,600
+يعني زي مثلاً حجم one microliter، يعني أنت حتى ما
+
+379
+00:27:35,600 --> 00:27:39,040
+بتشوفيه على الـ tip، يعني صعب تشوفيه على الـ tip إن أنت
+
+380
+00:27:39,040 --> 00:27:42,720
+أخذتِ أو ما أخذتِ، فالأفضل إن أنا آخذ حجم زيادة و
+
+381
+00:27:42,720 --> 00:27:45,560
+لما أفضي داخل الـ solution أو الوعاء اللي أنا بدي
+
+382
+00:27:45,560 --> 00:27:50,640
+أشتغل فيه، بفضي بالحجم اللي بدي إياه، إذا .. إذا لما
+
+383
+00:27:50,640 --> 00:27:56,550
+أنا أسحب الحجم بضغطة وأفرغه بضغطة، ضغطتين، معناها أنا بدي
+
+384
+00:27:56,550 --> 00:28:01,050
+آخذ exact volume وأفرغه بضغطتين عشان أضمن إن
+
+385
+00:28:01,050 --> 00:28:05,270
+أنا فرغته كويس، تمام؟ هذه طريقة forward pipetting،
+
+386
+00:28:05,270 --> 00:28:10,510
+لكن لما أنا أسحب الحجم بضغطتين وأفرغه بضغطة واحدة،
+
+387
+00:28:10,
+
+445
+00:32:24,510 --> 00:32:29,230
+tip مغمور داخل الـ solution و برفع إيدي بالتدريج عن
+
+446
+00:32:29,230 --> 00:32:32,670
+الـ plunger بسحبلي الـ volume طبعا هنا في الـ reverse
+
+447
+00:32:32,670 --> 00:32:37,190
+أنا ضغطت ضغطتين فهيسحبلي extra volume تمام؟ بعدين
+
+448
+00:32:37,190 --> 00:32:40,570
+بروح على الـ container اللي أنا بدي أفضي فيه و بضغط
+
+449
+00:32:40,570 --> 00:32:44,230
+ضغطة واحدة علشان أنزلي الـ solution اللي أنا بديها
+
+450
+00:32:44,230 --> 00:32:48,210
+فقط الحجم اللي بدي إياه فقط، و الـ extra هو و الـ tab
+
+451
+00:32:48,210 --> 00:32:50,730
+بتخلص منه في الـ waste من خلال أني أضغط على الـ
+
+452
+00:32:50,730 --> 00:32:54,590
+ejector و تنفصل الـ tab عن الـ pipet و تروح في حال
+
+453
+00:32:54,590 --> 00:33:00,370
+سبيلها تمام؟ أول حاجة مع .. يعني ملاحظات لاستخدام
+
+454
+00:33:00,370 --> 00:33:04,650
+الـ pipet، never point a pipet up ممنوع أنك أنتَ
+
+455
+00:33:04,650 --> 00:33:07,970
+تستخدمي الـ pipet تقليبيها بهذا الشكل بحيث أن
+
+456
+00:33:07,970 --> 00:33:13,600
+تكون الـ tip صارت فوق و الـ pipet تحت، طبعا و أنا
+
+457
+00:33:13,600 --> 00:33:16,300
+دايما لما أستخدم الـ pipet بتكون بهذا الاتجاه بحيث
+
+458
+00:33:16,300 --> 00:33:19,620
+أنّ الـ tip لا تكون تحت، لأنه لو أنا بدي أجلب الـ volume
+
+459
+00:33:19,620 --> 00:33:22,680
+اللي موجود في الـ tip راح يفوت داخل الـ pipet طبعا هذه
+
+460
+00:33:22,680 --> 00:33:28,420
+عبارة عن pipet طبعا و في كمان نظام الـ .. الـ ..
+
+461
+00:33:28,420 --> 00:33:33,460
+الـ  ذنبريكات، فبالتالي ممكن تصدأ داخل الـ .. يعني يصير
+
+462
+00:33:33,460 --> 00:33:37,300
+صدأ أو يصير فيه تلف و ممكن في محاليل بترسب بتعمل
+
+463
+00:33:37,300 --> 00:33:41,440
+رواسب بتعمل انسداد، فهذا بيأثر على دقة الـ pipet فهو
+
+464
+00:33:41,440 --> 00:33:45,340
+يُفضّل أني ممنوع أوجهها إلى أعلى بحيث أن
+
+465
+00:33:45,340 --> 00:33:48,580
+الـ liquid يفوت داخل الـ pipet و يعملها destroying
+
+466
+00:33:48,580 --> 00:33:53,060
+تمام؟ طيب when drawing liquid with a
+
+467
+00:33:53,060 --> 00:33:56,540
+pipet it always release the plunger slowly طبعا
+
+468
+00:33:56,540 --> 00:34:00,720
+لحظة لاحظتوا أنتم حتى بنبرة صوتي ركزت كتير منيح و
+
+469
+00:34:00,720 --> 00:34:04,020
+أنا بحكي لكم لما ترفع إيدك عن الـ plunger ارفعيه
+
+470
+00:34:04,020 --> 00:34:09,040
+slowly شوية شوية بالتدريج بهدوء ما أرفع إيدي فجأة
+
+471
+00:34:09,040 --> 00:34:13,800
+ليش؟ لأنّ هنا هي بتحكي لك أنّ الـ .. الـ .. الـ شغل
+
+472
+00:34:13,800 --> 00:34:19,280
+ترفع إيدك بشكل تدريجي أو بشكل بطيء عن الـ plunger
+
+473
+00:34:19,280 --> 00:34:24,080
+هيمنع من أنّه الـ liquid يصير له rushing into أنّه
+
+474
+00:34:24,080 --> 00:34:28,160
+يندفع داخل الـ pipet و بالتالي ممكن يسبب لها انسداد
+
+475
+00:34:28,160 --> 00:34:35,100
+أو صدأ أو تلف، فبالتالي مهم جدا طبعا أنّه أرفع إيدي
+
+476
+00:34:35,100 --> 00:34:38,020
+بالتدريج this is especially important with large
+
+477
+00:34:38,020 --> 00:34:41,860
+volumes يعني و هذا الشيء بنتبه له كتير منيح و أنا
+
+478
+00:34:41,860 --> 00:34:46,740
+بأخذ حجوم كبيرة، حجوم كبيرة معناها السائل هيندفع
+
+479
+00:34:46,740 --> 00:34:50,720
+بشكل قوي هو كميته كبيرة فهيفوت داخل الـ pipet هيصل ل
+
+480
+00:34:50,720 --> 00:34:55,240
+الـ pipet من جوا و يتلفها be sure you use the proper
+
+481
+00:34:55,240 --> 00:35:02,780
+size tip for each pipet حكينا أنّه لكل pipet طبعا لكل
+
+482
+00:35:02,780 --> 00:35:08,040
+pipet في عندي tip خاصة فيها تبعا لحجمها أو تبعا لـ
+
+483
+00:35:08,040 --> 00:35:12,380
+الـ range تبعها، فأنا كل pipet بأستخدم لها الـ tip الخاصة
+
+484
+00:35:12,380 --> 00:35:15,340
+فيها، ما بنفع مثلا مع الـ pipets الصغيرة أستخدم tip
+
+485
+00:35:15,340 --> 00:35:19,040
+كبيرة، أصلا مش راح تركب عليها، و ما بنفع مع الـ pipets
+
+486
+00:35:19,040 --> 00:35:21,980
+الكبيرة أستخدم tip صغيرة، مش راح تكفي للـ volume اللي
+
+487
+00:35:21,980 --> 00:35:26,520
+أنا بدي أنقله طبعا، فإذا كل pipet لها tip خاصة فيها
+
+488
+00:35:26,520 --> 00:35:30,740
+بأستخدمها always use a new tip for each different
+
+489
+00:35:30,740 --> 00:35:35,960
+liquid طبعا كل حجم بدي أنقله بأستخدم له a new tip
+
+490
+00:35:35,960 --> 00:35:40,020
+طبعا الـ tip جديدة، و حكينا أنّه هاي الـ tip أسمها
+
+491
+00:35:40,020 --> 00:35:44,460
+disposable طبعا فهيمر معنا إن شاء الله مصطلح
+
+492
+00:35:44,460 --> 00:35:48,360
+disposable و أذكركم فيه يعني تستخدم لمرة واحدة فقط
+
+493
+00:35:48,640 --> 00:35:52,580
+طيب use the correct pipet for the volume to be
+
+494
+00:35:52,580 --> 00:35:56,260
+dispensed يعني أنا بدي أستخدم الـ pipet، أختار الـ
+
+495
+00:35:56,260 --> 00:36:02,180
+pipet اللي الـ range تبعها تمام مناسب لـ .. الـ .. الـ
+
+496
+00:36:02,180 --> 00:36:04,980
+.. الـ pipet للـ حجم اللي بدي أنقله، هي مثلا هنا
+
+497
+00:36:04,980 --> 00:36:09,420
+بيحكي لك never use the متين لـ ألف microliter pipet
+
+498
+00:36:09,420 --> 00:36:12,720
+to dispense متين تمام هي هنا مثلا هي الـ range من
+
+499
+00:36:12,720 --> 00:36:18,210
+متين لـ ألف ما بأستخدمها عشان أنقل حجم متين لأنّ حكينا
+
+500
+00:36:18,210 --> 00:36:20,870
+إنّها مش حتكون دقيقة على lower limit لكن ممكن تكون
+
+501
+00:36:20,870 --> 00:36:23,630
+دقيقة على upper limit أو بتكون أدق على upper
+
+502
+00:36:23,630 --> 00:36:28,750
+limit تمام؟ و لو أنا بدي آخذ حجوم أقل من 200 أو
+
+503
+00:36:28,750 --> 00:36:33,790
+أكثر من ألف هذا إيش ممكن يعملي؟ يسبب لي damage للأداة
+
+504
+00:36:33,790 --> 00:36:39,710
+هذه فبالتالي لازم أكون حذرة و حريصة في استخدام الـ
+
+505
+00:36:39,710 --> 00:36:44,850
+pipet without the pipet withdraw the pipet from the
+
+506
+00:36:44,850 --> 00:36:48,290
+receiving vessels carefully sliding the tip along
+
+507
+00:36:48,290 --> 00:36:51,890
+the wall هلأ مجرد ما أنا أرفع الـ pipet بعد ما آخذ
+
+508
+00:36:51,890 --> 00:36:55,610
+الحجم اللي بدي إياه لازم أعمل حاجة اسمها sliding يعني
+
+509
+00:36:55,610 --> 00:37:00,750
+أنا بأمرّر الـ tip هادي بأمسحها على فتحة الـ container
+
+510
+00:37:00,750 --> 00:37:05,690
+يعني مثل هذا الشكل، يعني أرفع و أنزل الـ pipet على
+
+511
+00:37:05,690 --> 00:37:11,130
+فوهة أو فتحة الـ container تبع المحلول بحيث
+
+512
+00:37:11,130 --> 00:37:15,910
+أنّه أي شيء عالق على جدار الـ tip من برّا أتخلص منه
+
+513
+00:37:15,910 --> 00:37:19,330
+أنا بعنيني فقط الـ volume اللي أنا نقلته في الداخل
+
+514
+00:37:19,330 --> 00:37:23,670
+طبعا، فبالتالي عشان ما آخذ excess volume remove the
+
+515
+00:37:23,670 --> 00:37:30,770
+tip from the sample liquid تمام؟ و no liquid should
+
+516
+00:37:30,770 --> 00:37:35,130
+remain on the outside of the tip طبعا يعني لما ..
+
+517
+00:37:35,130 --> 00:37:40,130
+مجرد ما أنا أطلع من الـ .. الـ .. يعني آخذ الحجم
+
+518
+00:37:40,130 --> 00:37:43,410
+اللي بدي إياه و أطلع من الـ container مش لازم يضلّ
+
+519
+00:37:43,410 --> 00:37:47,910
+بقايا من الـ solution على الـ tip يُفضّل أني أمسح
+
+520
+00:37:47,910 --> 00:37:52,620
+باستخدام الـ wipe تمام؟ أو الـ .. الـ gauze أو أي قطعة
+
+521
+00:37:52,620 --> 00:37:58,780
+.. قطعة قطن أو clinics أو قطن أو شاش بأجفف الـ .. الـ
+
+522
+00:37:58,780 --> 00:38:02,780
+.. الـ pipet بأمسحها بأمرّرها عليها بحيث أني أجفف
+
+523
+00:38:02,780 --> 00:38:07,300
+أي بقايا عالقة عليها، ولكن يُفضّل أن أكون حريصة أنّه
+
+524
+00:38:07,300 --> 00:38:12,600
+هادي الـ .. الـ .. الـ pipet تماما ما تلامس فوهة الـ tip
+
+525
+00:38:12,600 --> 00:38:17,540
+ليش؟ لأنّها لو لامست فوهة الـ tip هذا absorbent، المحارم
+
+526
+00:38:17,540 --> 00:38:21,000
+و الـ clinics و الشيء بينشفّ السائل، فممكن ينشفّ
+
+527
+00:38:21,000 --> 00:38:25,820
+السائل اللي موجود داخل الـ tip و بالتالي بينقص الـ
+
+528
+00:38:25,820 --> 00:38:28,560
+volume اللي أخذته و بيكون أنا هيك عم بأخذ volumes
+
+529
+00:38:28,560 --> 00:38:32,260
+مش accurate و مش precise، تمام؟ مهم جدا لهاي
+
+530
+00:38:32,260 --> 00:38:37,440
+الـشغلة إذا بعد ما أنه أسحب الـ .. الـ .. الـ
+
+531
+00:38:37,440 --> 00:38:37,860
+الـ .. الـ .. الـ .. الـ .. الـ .. الـ .. الـ .. الـ
+
+532
+00:38:37,860 --> 00:38:38,800
+الـ .. الـ .. الـ .. الـ .. الـ .. الـ .. الـ .. الـ
+
+533
+00:38:38,800 --> 00:38:39,540
+الـ .. الـ .. الـ .. الـ .. الـ .. الـ .. الـ .. الـ
+
+534
+00:38:39,540 --> 00:38:39,680
+الـ .. الـ .. الـ .. الـ .. الـ .. الـ .. الـ .. الـ
+
+535
+00:38:39,680 --> 00:38:43,650
+الـ .. المش لازم no liquid should remain on the
+
+536
+00:38:43,650 --> 00:38:46,950
+outside مش لازم الـ liquid يضل على خارج الـ tip
+
+537
+00:38:46,950 --> 00:38:51,470
+فبالتالي بأمسح بقايا اللي .. اللي موجودة على الـ
+
+538
+00:38:51,470 --> 00:38:56,190
+outside of the tip باستخدام tissue, paper, gauze,
+
+539
+00:38:56,730 --> 00:39:01,130
+wipes أي شيء .. أي نوع محارم أو clinics بحيث أنّه ما
+
+540
+00:39:01,130 --> 00:39:04,790
+ألمس never touch the tip orifice ما أفتحها، ما ألمسش
+
+541
+00:39:04,910 --> 00:39:08,290
+الـ tip orifice يعني فتحة الـ .. الـ tip، الـ opening
+
+542
+00:39:08,290 --> 00:39:12,410
+تبع الـ tip طبعا ما ألمسها بحيث أنّه ما ينشف السائل و
+
+543
+00:39:12,410 --> 00:39:16,770
+بهيك أنا بكون عم بأخذ volume أقل من اللازم و بكون
+
+544
+00:39:16,770 --> 00:39:19,870
+مش .. مش accurate و مش precise في عملية الـ volume
+
+545
+00:39:19,870 --> 00:39:25,090
+transfer طبعا، طيب pre-rinsing إيش يعني كلمة pre-
+
+546
+00:39:25,090 --> 00:39:31,610
+rinsing؟ يُفضّل أني .. أنا لما بدي أنقل حجوم أن
+
+547
+00:39:31,610 --> 00:39:39,390
+أنا أبلّل يعني مثلا هنا يُفضّل إني أبلّل الـ .. الـ ..
+
+548
+00:39:39,390 --> 00:39:44,290
+الـ tip من جوا في .. في .. في السائل اللي بدي أنقله،
+
+549
+00:39:44,290 --> 00:39:47,510
+هاي معناها pre-rinsing طبعا؟ يعني أول حاجة بسحب أول
+
+550
+00:39:47,510 --> 00:39:52,330
+volume و بتخلص منه بأكبّه، هيك أنا بضمن إنّه جزء من
+
+551
+00:39:52,330 --> 00:39:56,570
+السائل لمس الـ .. الـ .. الـ tip من الداخل فبالتالي
+
+552
+00:39:56,570 --> 00:40:02,570
+صارت الـ tip أصبحت مبللة، طيب فلما أجي أنقل volume
+
+553
+00:40:03,230 --> 00:40:06,390
+و آخذ الـ volume و أنقله هيك بكون أنا عم بأخذ
+
+554
+00:40:06,390 --> 00:40:10,490
+volume بشكل أدق، ليش؟ لأنّه لو .. لو أنا ما عملت الـ
+
+555
+00:40:10,490 --> 00:40:14,490
+pre-rinsing هذه طبعا لو ما عملت pre-rinsing أخذت
+
+556
+00:40:14,490 --> 00:40:18,350
+volume و روحت فضيته في جزء من السائل اللي أنا
+
+557
+00:40:18,350 --> 00:40:24,370
+أخذته بيضلّ ملتصق بجدران الـ tip مبلّل لها وهذا جزء
+
+558
+00:40:24,370 --> 00:40:29,190
+يعني في نسبة فقد للـ volume، في نسبة error طبعا لكن
+
+559
+00:40:29,190 --> 00:40:34,490
+لما أنا أستخدم tip already مبللة يعني tip أنا سحبت
+
+560
+00:40:34,490 --> 00:40:36,970
+أول volume فيها و كبيته و بعدين سحبت الـ volume
+
+561
+00:40:36,970 --> 00:40:40,290
+اللي بدي أستخدمه، الـ volume الفعلي، معناها أنا بلّلت
+
+562
+00:40:40,290 --> 00:40:45,850
+الـ tip من الداخل فبالتالي فبطل في عندي نسبة فقد من
+
+563
+00:40:45,850 --> 00:40:49,890
+الحجم و بطل في عندي نسبة الـ error هذه معناها كلمة
+
+564
+00:40:49,890 --> 00:40:53,670
+pre-rinsing minimize أسمها الـ variances
+
+565
+00:40:53,670 --> 00:40:59,560
+الاختلافات في الـ .. الـ .. الحجوم طبعا when by
+
+566
+00:40:59,560 --> 00:41:03,080
+pipetting some solutions زي الـ serum اللي هي العينات
+
+567
+00:41:03,080 --> 00:41:08,260
+يعني الشخ.. المرضى، الـ serum، البلازما، الـ protein
+
+568
+00:41:08,260 --> 00:41:11,300
+containing solution المحاليل اللي بتحتوي على
+
+569
+00:41:11,300 --> 00:41:14,420
+البروتينات، الـ organic solvents، المُذيبات العضوية
+
+570
+00:41:14,420 --> 00:41:19,700
+هذه طبعا في جزء منها بيكون حكينا أنّه حيصير فيه فيلم
+
+571
+00:41:19,700 --> 00:41:24,500
+فيه جزء منها حيبقى، حيلامس الـ inside of the tip و
+
+572
+00:41:24,500 --> 00:41:29,200
+هذا حكينا اسمه error فبالتالي لما أنا أعمل عملية
+
+573
+00:41:29,200 --> 00:41:33,540
+pre-rinsing فبالتالي أنا بقلّل الـ error هذه من خلال
+
+574
+00:41:33,540 --> 00:41:39,120
+أنّي خلاص استخدمت already جزء بيبت مبللة جزء ..
+
+575
+00:41:39,120 --> 00:41:43,040
+بيبت عملنا لها pre-rinsing فبالتالي مش حيكون في
+
+576
+00:41:43,040 --> 00:41:47,400
+عندي فقد في الـ volume و مش حيكون في عندي نسبة
+
+577
+00:41:47,400 --> 00:41:51,780
+error طيب when pipetting concentrated acid or
+
+578
+00:41:51,780 --> 00:41:56,100
+highly corrosive solution such as halide salts
+
+579
+00:41:56,100 --> 00:42:01,540
+it's recommended to that pipet be disassembled
+
+580
+00:42:01,540 --> 00:42:07,680
+after used to inspect the shaft و المكونات تبعها،
+
+581
+00:42:07,680 --> 00:42:11,800
+هلأ بيحكي لك أنّه لما بنستخدم بعض المحاليل اللي هي
+
+582
+00:42:11,800 --> 00:42:18,100
+corrosive تمام، حارقة أو يعني مؤذية وبيطير منها
+
+583
+00:42:18,100 --> 00:42:24,160
+أبخرة أو يعني فيه بيصعد منها قُترانات أو أبخرة أو
+
+584
+00:42:24,160 --> 00:42:28,960
+رذاذ زي الـ .. الـ .. الـ halide salts هاي يُفضّل أني
+
+585
+00:42:28,960 --> 00:42:32,620
+أفكّ الـ pipet و أنظفها من الداخل و أرجع أركّبها ثانية
+
+586
+00:42:32,620 --> 00:42:38,940
+عشان أتأكّد إنّها هي يعني ما رح تتعطل ما رح تخرب، الـ
+
+587
+00:42:38,940 --> 00:42:44,050
+air bubble طبعا يعني أن في عندي تقطّع في السائل اللي
+
+588
+00:42:44,050 --> 00:42:47,170
+أنا عم بأخذه في هنا فيه فقاعات في فيه .. فيه هوا
+
+589
+00:42:47,170 --> 00:42:52,670
+تمام؟ طب هذا الـ .. الـ .. الهواء إيش سببه؟ سببه أول
+
+590
+00:42:52,670 --> 00:42:55,970
+حاجة إما إني رفعت إيدي عن الـ plunger بشكل سريع
+
+591
+00:42:55,970 --> 00:43:00,010
+أو كانت الـ tip مش مغمورة كويس في الـ solution، سحبت
+
+592
+00:43:00,010 --> 00:43:03,550
+من عن السطح فبالتالي بما إني سحبت عن السطح معناها
+
+593
+00:43:03,550 --> 00:43:10,810
+صار في فرصة إنّه يدخل air bubble تمام ممكن يكون إنّه
+
+594
+00:43:11,460 --> 00:43:19,940
+سببها الـ .. الـ .. يعني السائل نفسه يكون يعني فوّامي
+
+595
+00:43:19,940 --> 00:43:26,480
+بي.. بيكون رغوة فبالتالي أنا ما اتبعت التعليمات لـ
+
+596
+00:43:26,480 --> 00:43:31,360
+.. لـ .. لـ الـ pipetting من فوّامي reagent
+
+597
+00:43:31,360 --> 00:43:36,240
+فبالتالي ممكن تظهر عندي air bubble طبعا الـ .. الـ
+
+598
+00:43:36,240 --> 00:43:40,190
+.. الـ air bubble إذا ظهرت طبعا، طبعا أول حاجة إذا
+
+599
+00:43:40,190 --> 00:43:43,910
+ظهرت في .. في الـ pipet بأبدل solution و برجع بأخذ
+
+600
+00:43:43,910 --> 00:43:47,490
+مرة ثانية، ضلّت موجودة معناها بأبدل أتخلص من الـ tip و
+
+601
+00:43:47,490 --> 00:43:51,090
+أركّب tip جديدة و آخذ volume مع الـ التزام
+
+602
+00:43:51,090 --> 00:43:56,410
+بالـتعليمات حتى أتجنّب من ظهور air bubble، ليش؟ لأنّ
+
+603
+00:43:56,410 --> 00:43:59,150
+الـ air bubble طبعا هذه محتلة جزء من الحجم اللي
+
+604
+00:43:59,150 --> 00:44:03,580
+موجود، يعني هذا المفروض كله يكون سائل، طبعا فوجود air
+
+605
+00:44:03,580 --> 00:44:06,860
+bubble وجود تقطّع في السائل يعني فيه هواء فبالتالي
+
+606
+00:44:06,860 --> 00:44:09,900
+الهواء محتل حجم، إذا أنا مش ماخدة الحجم المناسب
+
+607
+00:44:09,900 --> 00:44:14,360
+ماخدة أقل منه، إذا أنا مش accurate و مش precise طبعا
+
+608
+00:44:14,360 --> 00:44:19,420
+هذا بالنسبة للـ pipet طيب خلينا بس قبل ما نشوف الـ
+
+609
+00:44:19,420 --> 00:44:23,580
+calibration للـ pipet نتابع مع بعض نشوف هذا
+
+610
+00:44:23,580 --> 00:44:30,040
+الفيديو بيعلمنا كيف أنا بدي أستخدم الـ .. الـ pipet
+
+611
+00:44:30,040 --> 00:44:34,000
+و هنرجع نح
+
+667
+00:48:55,290 --> 00:48:59,850
+تمام هي هنا فضّة الـ volume هي هنا ضغطة، هي ضغطتين
+
+668
+00:48:59,850 --> 00:49:03,690
+تمام، تعلمنا الضغطة والضغطتين، وهي هنا شايفين كده
+
+669
+00:49:03,690 --> 00:49:08,430
+أنا بفوت ضغطة، وبعدين ب... بسحب الـ... ب... بترك
+
+670
+00:49:08,430 --> 00:49:12,630
+إيدي شوية شوية عن البلانجر، ما بنفع أنا أفوت مش ضغطة
+
+671
+00:49:12,630 --> 00:49:16,250
+وأضغط جوا الـ solution ليش؟ لإنه ساعتها أنا بكون
+
+672
+00:49:16,250 --> 00:49:18,890
+بدخل هواء جوا الـ solution إذا هو من النوع الفومي
+
+673
+00:49:18,890 --> 00:49:21,890
+اللي بيكون رغوة، هيصير فيه رغوات ويصير في عندي air
+
+674
+00:49:21,890 --> 00:49:26,950
+bubble ومشاكل في نقل الحجوم، طبعا طيب هلأ أنا بعد
+
+675
+00:49:26,950 --> 00:49:29,730
+ما أنا فضيت الـ volume، ضغطت على البلانجر وفضيت الـ
+
+676
+00:49:29,730 --> 00:49:32,350
+volume، إيش بده أعمل؟ بده أضغط على الـ ejector عشان
+
+677
+00:49:32,350 --> 00:49:36,370
+أتخلص من الـ tip، شايفين مجرد ما أضغط عليها الـ... الـ
+
+678
+00:49:36,370 --> 00:49:40,890
+... الـ tip بتفصل عن الـ bibert وبت... بتم التخلص منها
+
+679
+00:49:40,890 --> 00:49:46,460
+طبعا هذا بالنسبة للـ... للـ... للـ pipette، إذا أول
+
+680
+00:49:46,460 --> 00:49:52,560
+حاجة بنحدد الحجم، ب... أركب الـ tip، بعمل pre-rinsing
+
+681
+00:49:52,560 --> 00:49:58,200
+ببلّلها تمام، وبعدين باخد الحجم اللي بدّي اياه
+
+682
+00:49:58,200 --> 00:50:01,960
+سواء كان باخده بطريقة الـ... الـ... الـ forward
+
+683
+00:50:01,960 --> 00:50:06,000
+pipetting أو الـ reverse pipetting حسب اللي أنا الحجم
+
+684
+00:50:06,000 --> 00:50:09,930
+اللي الـ... الـ... أو السائل اللي أنا بتعامل معاه
+
+685
+00:50:09,930 --> 00:50:15,030
+بانقله من وإلى، بعد هيك بتخلص من الـ tip بهذا الشكل
+
+686
+00:50:15,030 --> 00:50:21,550
+تمام؟ إن شاء الله هيكون شغلنا على الـ... الـ pipette و
+
+687
+00:50:21,550 --> 00:50:24,630
+طبعا معظم... كل الفحوصات بتنعمل باستخدام الـ pipette
+
+688
+00:50:24,630 --> 00:50:28,630
+أنا بدي أضيف حجم من الـ reagent وحجم من العينة، حجم
+
+689
+00:50:28,630 --> 00:50:31,510
+من الـ standard وحجم من الـ reagent، كل شغلي
+
+690
+00:50:31,510 --> 00:50:33,710
+باستخدام الـ pipette، إذا هذه صديقتنا، هذه اللي
+
+691
+00:50:33,710 --> 00:50:36,390
+هترافقنا إن شاء الله من سنة تانية لسنة رابعة لجهة
+
+692
+00:50:36,390 --> 00:50:39,950
+الأداء، فبالتالي لازم نكون دقيقين في استخدامها، و
+
+693
+00:50:39,950 --> 00:50:42,690
+عشان... عشان نكون دقيقين باستخدامها وتكون الـ pipette
+
+694
+00:50:42,690 --> 00:50:46,370
+هادئة، إحنا بنعمل لها صيانة وبنضمن دقتها باستمرار
+
+695
+00:50:46,370 --> 00:50:49,530
+لازم أنه أنا أعمل لها calibration، إيش يعني
+
+696
+00:50:49,530 --> 00:50:52,890
+calibration؟ يعني إعادة ضبط وتهيئة، علشان أتأكد من
+
+697
+00:50:52,890 --> 00:50:57,070
+شغلها، إن هي عم تاخد لي شغل دقيق، طبعا كيف أنا بدي
+
+698
+00:50:57,070 --> 00:51:01,660
+أخد الـ... الـ... أكيد أعمل لها calibration؟ بدي
+
+699
+00:51:01,660 --> 00:51:06,200
+أستخدمها يعني، أو أظبط شغلها، أو أتأكد من إنها عم
+
+700
+00:51:06,200 --> 00:51:10,820
+تاخد volumes بشكل دقيق، باستخدام حجوم الماء الـ
+
+701
+00:51:10,820 --> 00:51:14,960
+distilled water، تمام؟ هنا بيحكي لي إنه تحت درجة
+
+702
+00:51:14,960 --> 00:51:21,200
+الحرارة والضغط الثابت، الكثافة الماء أيضا ثابتة
+
+703
+00:51:21,200 --> 00:51:26,860
+فالحجم من الماء، تمام، ممكن أنا يعني 1 ml من الميّة
+
+704
+00:51:26,860 --> 00:51:31,820
+بيساوي 1 gram من الميّة، تمام، الـ... الـ 1 ml الـ
+
+705
+00:51:31,820 --> 00:51:36,360
+volume، 1 ml لو أنا جيت وزنته تحت درجة حرارة وضغط
+
+706
+00:51:36,360 --> 00:51:40,400
+ثابت، وكثافة ثابتة للماء، فهي... الـ... الـ 1 ml
+
+707
+00:51:40,400 --> 00:51:46,710
+هيعطيني 1 gram من الـ water، تمام؟ طيب، فبالتالي الـ
+
+708
+00:51:46,710 --> 00:51:51,330
+... الـ calibration للـ pipette لازم أنا أكون بس يعني
+
+709
+00:51:51,330 --> 00:51:54,390
+بدي أعرف الـ volume اللي عم تاخده، حجم الماء اللي
+
+710
+00:51:54,390 --> 00:51:59,890
+بتاخده، وهل هو accurate أو precise، وطبعا هذا
+
+711
+00:51:59,890 --> 00:52:06,910
+بيتأثر بدرجة حرارة الغرفة، وأيضا الضغط الجوي، و
+
+712
+00:52:06,910 --> 00:52:10,190
+الرطوبة، نسبة الرطوبة اللي موجودة في الوسط أو في
+
+713
+00:52:10,190 --> 00:52:14,320
+البيئة المحيطة لإجراء التجربة، عشان هيك أنا بستخدم
+
+714
+00:52:14,320 --> 00:52:17,980
+factor أو ثابت اسمه z factor في الحسابات، طبعا
+
+715
+00:52:17,980 --> 00:52:23,340
+هنحكي عنه كمان شوية، هذا الـ z factor، فالفكرة طبعا
+
+716
+00:52:23,340 --> 00:52:27,660
+أنه أنا بدي آخد 1 ml من الـ distilled water، يعني
+
+717
+00:52:27,660 --> 00:52:30,980
+بدي أجيب الـ pipette، بدي أعمل لها setting، بدي...
+
+718
+00:52:30,980 --> 00:52:35,700
+أضبط... أضبطها على 1 ml، أركب tip، آخد، أعمل الـ pre-
+
+719
+00:52:35,700 --> 00:52:39,920
+rinsing، أبلل الـ tip من... وأتخلص من الـ solution
+
+720
+00:52:39,920 --> 00:52:44,350
+اللي اخذته في المرة الأولى، بعد هيك إيش بدي أعمل؟
+
+721
+00:52:44,350 --> 00:52:50,110
+بدي آخد 1 ml من distilled water وأحطه في cup، الـ
+
+722
+00:52:50,110 --> 00:52:53,990
+cup هذا بدي أكون مسفر عليه في الميزان، بدي أسفر
+
+723
+00:52:53,990 --> 00:52:57,590
+عليه وأحط الـ 1 ml وأشوف الوزنة تبعته، الوزنة
+
+724
+00:52:57,590 --> 00:53:00,090
+تبعته حتكون، إذا هو مسفر على الـ cup، فالوزنة اللي
+
+725
+00:53:00,090 --> 00:53:05,050
+حتطلع معي هي وزنة الـ 1 ml، المفروض تكون قريبة
+
+726
+00:53:05,050 --> 00:53:08,470
+لـ 1 gram، تمام؟ المفروض إنها تكون قريبة لـ 1
+
+727
+00:53:08,470 --> 00:53:13,310
+gram أو... أو يعني exactly 1 gram، طبعا فعشان إن
+
+728
+00:53:13,310 --> 00:53:16,130
+إحنا عندنا درجة الحرارة والضغط الجوي والـ
+
+729
+00:53:16,130 --> 00:53:21,070
+humidity متغيرة، فأنا بروح بدرب الوزن هذه تبعته بالـ
+
+730
+00:53:21,070 --> 00:53:25,390
+z factor وفي جدول في آخر الـ... الـ... الـ slide
+
+731
+00:53:25,390 --> 00:53:29,590
+بعلّمني كيف أنا أختار الـ z factor المناسب اللي بدي
+
+732
+00:53:29,590 --> 00:53:36,880
+أدرّب فيه القراءات تبعته، طبعا، فإذا الـ... الحجم هي هنا
+
+733
+00:53:36,880 --> 00:53:41,300
+مثلا، إيش بيلزمني؟ sorry، إيش بيلزمني للتجربة تبعته؟
+
+734
+00:53:41,300 --> 00:53:46,100
+بيلزمني pipette و tip، بيلزمني beaker أو cup لحتى أحطه
+
+735
+00:53:46,100 --> 00:53:49,220
+على الميزان وأصفر عليه، تمام؟ بيلزمني distilled
+
+736
+00:53:49,220 --> 00:53:54,260
+water، بيلزمني أقيس درجة الحرارة وميزان وأقيس الـ
+
+737
+00:53:54,260 --> 00:54:00,850
+pressure، الضغط، عشان أطلع z factor، أول شيء بدي أقيس
+
+738
+00:54:00,850 --> 00:54:07,510
+درجة حرارة الماء وأسجلها، بعد هيك باستخدام beaker
+
+739
+00:54:07,510 --> 00:54:10,950
+طبعا أنه أنا بدي أحطه على الـ... طبعا مش راح أقرأ
+
+740
+00:54:10,950 --> 00:54:18,900
+كل الخطوات، هاي راح أختصرها، باستخدام الـ beaker أو الـ
+
+741
+00:54:18,900 --> 00:54:23,080
+cup، راح أحطه على الـ analytical balance وراح أصفر
+
+742
+00:54:23,080 --> 00:54:27,080
+عليه، طبعا دي خلي... أ... أ... أخلي الميزان يعتبر إن
+
+743
+00:54:27,080 --> 00:54:32,040
+وزن الـ cup هذا zero، طبعا، بعد هيك بدي أعمل pre...
+
+744
+00:54:32,040 --> 00:54:35,520
+بدي أركب tip للـ pipette، وبعد ما أكون أنا حددت الحجم
+
+745
+00:54:35,520 --> 00:54:40,840
+المناسب إلها اللي هو 1 ml، يعني 1000 microliter، و
+
+746
+00:54:40,840 --> 00:54:44,940
+بعد هيك بدي أعمل pre-rinsing، عرفنا إيش يعني pre-
+
+747
+00:54:44,940 --> 00:54:49,040
+rinsing؟ يعني أنا بدي أسحب وأُسبيريت وأُسفِن، يعني
+
+748
+00:54:49,040 --> 00:54:53,640
+بدي أسحب وأتخلص من الحجم، على أساس ممكن أعيد هذه
+
+749
+00:54:53,640 --> 00:54:56,860
+الخطوة تلات مرات، على أساس أتأكد إنه الـ... الـ...
+
+750
+00:54:56,860 --> 00:54:59,580
+الـ tip صارت مبللة من الداخل، يعني مش حيكون في عندي لا... لا
+
+751
+00:54:59,580 --> 00:55:05,320
+... لا فقط ولا error في الـ volume، تمام، وبعد هيك إيش
+
+752
+00:55:05,320 --> 00:55:09,880
+بدي أعمل؟ بدي أضغط على الـ... الـ... الـ... الـ... الـ
+
+753
+00:55:09,880 --> 00:55:11,120
+... الـ... الـ... الـ... الـ... الـ... الـ... الـ...
+
+754
+00:55:11,120 --> 00:55:11,380
+الـ... الـ... الـ... الـ... الـ... الـ... الـ... الـ...
+
+755
+00:55:11,380 --> 00:55:11,580
+... الـ... الـ... الـ... الـ... الـ... الـ... الـ... الـ...
+
+756
+00:55:11,580 --> 00:55:13,040
+الـ... الـ... الـ... الـ... الـ... الـ... الـ... الـ... الـ...
+
+757
+00:55:13,040 --> 00:55:13,160
+... الـ... الـ... الـ... الـ... الـ... الـ... الـ... الـ...
+
+758
+00:55:13,160 --> 00:55:13,660
+الـ... الـ... الـ... الـ... الـ... الـ... الـ... الـ... الـ...
+
+759
+00:55:13,660 --> 00:55:14,400
+الـ... الـ... الـ... الـ... الـ... الـ... الـ... الـ... الـ...
+
+760
+00:55:14,400 --> 00:55:21,420
+... الـ... الـ...
+
+761
+00:55:21,420 --> 00:55:21,440
+الـ... الـ... الـ... الـ... الـ... الـ... الـ... الـ... الـ...
+
+762
+00:55:21,440 --> 00:55:25,140
+... الـ...
+
+763
+00:55:34,190 --> 00:55:40,910
+عشان السائل يفوت داخل الـ tip أيضا بشكل متأني، يعني
+
+764
+00:55:40,910 --> 00:55:44,210
+أنه ما يصير فيه اندفاع، لا solution ولا air bubble
+
+765
+00:55:44,210 --> 00:55:48,260
+والمشاكل اللي ممكن تحدث في الـ pipetting، تمام؟ هيك
+
+766
+00:55:48,260 --> 00:55:51,980
+أنا أخدت الحجم اللي بدي اياه، بروح بفضيه في الـ beaker
+
+767
+00:55:51,980 --> 00:55:55,340
+أو الـ cup اللي موجود على الميزان، وبآخد القراءة
+
+768
+00:55:55,340 --> 00:55:59,760
+تمام؟ بعد هيك ممكن أنا أرجع أصفر على الـ cup والـ
+
+769
+00:55:59,760 --> 00:56:03,420
+... الـ volume اللي موجود فيه، أو أن أنا أستخدم cup
+
+770
+00:56:03,420 --> 00:56:08,100
+جديد، إيه الخيار؟ بأصفر وأعيد الخطوة مرة ثانية، باخد
+
+771
+00:56:08,100 --> 00:56:13,560
+1 ml بالـ pipette وبفضيه وبآخد كمان وزنة، وكمان
+
+772
+00:56:13,560 --> 00:56:17,340
+مرة تالتة، باخد 1 ml وبفضيه في الميزان وبآخد
+
+773
+00:56:17,340 --> 00:56:22,320
+وزنة تالتة، فإذا صار في عندي تلات وزنات لتلات حجوم
+
+774
+00:56:22,320 --> 00:56:25,400
+تمام، فإيش بدي أعمل في التلات وزنات هدول؟ بدي أجيب
+
+775
+00:56:25,400 --> 00:56:28,740
+الـ average تبعهم، يعني بدي أجيب مجموعهم على تلاتة
+
+776
+00:56:29,100 --> 00:56:34,400
+تمام؟ الـ... هذه الـ... شو اسمه؟ الـ... يعني تَكَمّلت
+
+777
+00:56:34,400 --> 00:56:37,220
+التجربة اللي أنا حكيتها، يعني إنه... إنه أعيدي
+
+778
+00:56:37,220 --> 00:56:41,380
+الخطوات تلات مرات، وجيبي القراءة، هي هنا مثلا
+
+779
+00:56:41,380 --> 00:56:44,340
+بيحكي لي ممكن تعدّيها أربع عشرة مرة، حسب، يعني ممكن
+
+780
+00:56:44,340 --> 00:56:48,200
+تعدّيها تلات مرات، أربع مرات، خمس مرات، المهم بالنهاية
+
+781
+00:56:48,200 --> 00:56:51,640
+الوزنات اللي تطلع معك بدك تاخدي لها average، إيش
+
+782
+00:56:51,640 --> 00:56:56,440
+يعني average؟ يعني مجموعها على عددها، وطلع قراءة
+
+783
+00:56:56,440 --> 00:56:59,680
+واحدة، القراءة واحدة، هي هذه اللي بدك تروحي تعاودي
+
+784
+00:56:59,680 --> 00:57:02,940
+فيها بالقانون، الـ weight أو الـ average of weight
+
+785
+00:57:02,940 --> 00:57:06,180
+اللي طلع معك، وبدك تضربيه في الـ z factor اللي هو
+
+786
+00:57:06,180 --> 00:57:10,980
+معامل اللي هو الحرارة والضغط، تمام، اللي موجود عندك
+
+787
+00:57:10,980 --> 00:57:14,980
+وبالنهاية بحسب الـ volume، أنا أخدت الـ pipette كانت
+
+788
+00:57:14,980 --> 00:57:18,940
+بتحكي لي إن أنا باخد 1 ml على الشاشة تبع الـ pipette
+
+789
+00:57:18,940 --> 00:57:22,840
+مكتوب 1 ml، فالمفروض إن هذا الكلام، الـ weight في الـ
+
+790
+00:57:22,840 --> 00:57:27,020
+z factor يكون 1 ml أيضا، يعني أو قريب لـ 1 ml
+
+791
+00:57:27,020 --> 00:57:34,050
+فممكن أنا أطبق قوانين التحليلية، يعني هي إن أنا بجيب
+
+792
+00:57:34,050 --> 00:57:39,050
+الـ average لقراءات الوزنة، اللي هي مجموع القراءات
+
+793
+00:57:39,050 --> 00:57:43,170
+على عددها، تمام، ممكن أطبق أيضا، أحسب الـ standard
+
+794
+00:57:43,170 --> 00:57:47,870
+deviation، أجيب الـ accuracy، يعني القيمة الوزنة اللي
+
+795
+00:57:47,870 --> 00:57:50,610
+طلعت معي، واللي المفروض تطلع، مثلا طلعت معي
+
+796
+00:57:50,610 --> 00:57:54,690
+0.999 الوزنة، والمفروض إنها تطلع 1 milligram
+
+797
+00:57:54,690 --> 00:57:58,280
+بحسبها، بشوف... بشوف نسبة الـ accuracy تبع القيمة
+
+798
+00:57:58,280 --> 00:58:02,100
+تبعته، والـ coefficient of variation اللي هي...
+
+799
+00:58:02,100 --> 00:58:04,560
+اللي هي عبارة عن الـ standard deviation على الـ mean
+
+800
+00:58:04,560 --> 00:58:11,920
+ضرب 100%، لازم الـ accuracy هذه تطلع من 99 ل 101%، و
+
+801
+00:58:11,920 --> 00:58:16,470
+لازم الـ coefficient of variation يكون أقل من 1
+
+802
+00:58:16,470 --> 00:58:19,690
+في المئة، هذا يكون أقل من 1 في المئة عشان أقول
+
+803
+00:58:19,690 --> 00:58:22,290
+إن الـ pipette هذه accurate و precise وبتعطيني
+
+804
+00:58:22,290 --> 00:58:25,570
+volumes دقيقة، والحجم اللي أنا باخده هو نفسه اللي
+
+805
+00:58:25,570 --> 00:58:28,990
+موجود على الشاشة وشغلها مية مية، إذا أنا لما بدي
+
+806
+00:58:28,990 --> 00:58:31,930
+أجري فحوصات وبدي أنقل volume باستخدام هذه الـ pipette
+
+807
+00:58:31,930 --> 00:58:36,740
+إذا أنا عم بشتغل بشكل صحيح وبشكل دقيق، طبعا هذا
+
+808
+00:58:36,740 --> 00:58:40,740
+الجدول اللي أنا من خلاله بختار مثلا الـ... الـ z
+
+809
+00:58:40,740 --> 00:58:45,180
+value، أو اللي هو معامل الـ... الحرارة والضغط، طبعا
+
+810
+00:58:45,180 --> 00:58:47,980
+الـ z value، مثلا أنا بشوف إيش الضغط اللي موجود عندي
+
+811
+00:58:47,980 --> 00:58:50,040
+حسب المنطقة اللي أنا... اللي أنا فيها، لأن أفرض
+
+812
+00:58:50,040 --> 00:58:54,620
+مثلا إن أنا موجودة في منطقة ضغطها 1067
+
+813
+00:58:54,620 --> 00:58:59,420
+و60 milli bar، طبعا، ودرجة الحرارة عندي 25 و
+
+814
+00:58:59,420 --> 00:59:04,240
+20، بروح هنا، هي 25، بمشي لعند مين؟ لعند
+
+815
+00:59:04,240 --> 00:59:08,100
+الـ... الـ... الـ... المنطقة اللي هي الـ... اللي فيها
+
+816
+00:59:08,100 --> 00:59:11,600
+الضغط تبعي، تمام؟ اللي فيها الضغط تبعي، هنا هيك هي
+
+817
+00:59:11,600 --> 00:59:15,480
+هذه القيمة، تقاطع درجة الحرارة اللي بدي إياها مع الضغط
+
+818
+00:59:15,480 --> 00:59:18,480
+اللي... اللي بدي إياه، هي هذه قيمة الـ z factor اللي
+
+819
+00:59:18,480 --> 00:59:22,300
+بروح ب... ب... أطبق فيها، تمام؟ هي هذه قيمة الـ z
+
+820
+00:59:22,300 --> 00:59:26,060
+factor اللي بروح ب... ب... أطبق فيها وبحسب حسابات تبعته، زي
+
+821
+00:59:26,060 --> 00:59:29,820
+ما حكينا، لو أنا عدّيت التجربة مرة، مرتين، تلاتة، أربع
+
+822
+00:59:29,820 --> 00:59:34,900
+عشرة، وحصلت على مثلا عشر قيم، باخد الـ average تبعهم
+
+823
+00:59:34,900 --> 00:59:39,100
+بضربهم بالـ z factor، بحصل على الـ volume وبطبق في
+
+824
+00:59:39,100 --> 00:59:43,180
+القوانين تبعته، القيمة اللي أنا جبتها على القيمة
+
+825
+00:59:43,180 --> 00:59:46,570
+اللي المفروض تطلع، ضرب 100%، بحسب الـ accuracy، بحسب الـ
+
+826
+00:59:46,570 --> 00:59:50,450
+coefficient of variation، بشوف نسبة دقة وصحة الـ...
+
+827
+00:59:50,450 --> 00:59:54,070
+الـ pipette تبعي، وبحكم على شغلها، وهل إنها محتاجة
+
+828
+00:59:54,070 --> 01:00:00,690
+إنها يتم تصليحها أو إنه لأ، مناسبة وتستخدم يعني
+
+829
+01:00:00,690 --> 01:00:05,350
+موثوقة للاستخدام، تمام؟ بهيك بن
\ No newline at end of file
diff --git a/PL9fwy3NUQKwY9jl8xw-bWkVZkWcGGyspd/S5RQk6QZXf4_postprocess.srt b/PL9fwy3NUQKwY9jl8xw-bWkVZkWcGGyspd/S5RQk6QZXf4_postprocess.srt
new file mode 100644
index 0000000000000000000000000000000000000000..c6913293b2e74394ceed347cdcb2d7be5230b8d2
--- /dev/null
+++ b/PL9fwy3NUQKwY9jl8xw-bWkVZkWcGGyspd/S5RQk6QZXf4_postprocess.srt
@@ -0,0 +1,3344 @@
+1
+00:00:04,520 --> 00:00:07,580
+السلام عليكم ورحمة الله وبركاته طالبتي اليوم ان
+
+2
+00:00:07,580 --> 00:00:12,060
+شاء الله رح ناخد معملنا رقم اتنين بعنوان automatic
+
+3
+00:00:12,060 --> 00:00:15,600
+by-produce and calibration معمل رقم اتنين فيه مساق
+
+4
+00:00:15,600 --> 00:00:20,760
+مقدمة في التحليل الطبية طبعا هذا كان اول موضوع كان
+
+5
+00:00:20,760 --> 00:00:25,100
+المفروض ان احنا ناخده عملي و نشتغل بادانا ولكن
+
+6
+00:00:25,100 --> 00:00:30,440
+للأسف نظرا لزروف المحيطة فيناراح ناخد ان شاء الله
+
+7
+00:00:30,440 --> 00:00:33,660
+.. راح ناخد النظري تبعه و ان شاء الله بعودتنا
+
+8
+00:00:33,660 --> 00:00:38,460
+للمختبرات بناخد التطبيق العملي لإله فاليوم ان شاء
+
+9
+00:00:38,460 --> 00:00:41,060
+الله موضوعنا عن ال automatic by bit use and
+
+10
+00:00:41,060 --> 00:00:44,280
+calibration خلينا نشوف إيش يعني automatic by bit
+
+11
+00:00:44,280 --> 00:00:47,480
+هلأ احنا المحاضرة الماضية خلينا بس نحكي هالنقطة
+
+12
+00:00:47,790 --> 00:00:53,150
+المحاضرة الماضية كانت بتحكي عن ال .. ال .. ال
+
+13
+00:00:53,150 --> 00:00:58,270
+volumes و الحجوم و المحاليل و كيف أنا بتعامل مع
+
+14
+00:00:58,270 --> 00:01:01,110
+المحاليل هاي و إيش أنواع المحاليل اللي تستخدم في
+
+15
+00:01:01,110 --> 00:01:05,930
+ال medical laboratories و .. و كيف أنا أخسف هاي
+
+16
+00:01:05,930 --> 00:01:10,690
+المحاليل، تمام؟طيب لما انا .. انا بدي أجي أنقل
+
+17
+00:01:10,690 --> 00:01:13,950
+المحلول من إلى يعني مثلا انا عندي عينة بدي
+
+18
+00:01:13,950 --> 00:01:17,970
+أضيفيلها reagent علشان أعمل فحص معين أو بدي أاخد
+
+19
+00:01:17,970 --> 00:01:22,130
+.. بدي أنقل حجوم علشان أعمل dilution زي ما أخدنا
+
+20
+00:01:22,130 --> 00:01:25,800
+ال .. مسائل ال dilution المحاضرة الماضيةكيف أنا
+
+21
+00:01:25,800 --> 00:01:29,140
+بدي أنقلها؟ هنا بال medical laboratories
+
+22
+00:01:29,140 --> 00:01:32,660
+بالمختبرات الطبية احنا بنستخدم اشي اسمه automatic
+
+23
+00:01:32,660 --> 00:01:37,100
+pipet اللي هو الأداة اللي موجودة بهذه الصورة اسمها
+
+24
+00:01:37,100 --> 00:01:40,740
+automatic pipet خلينا نشوف اش يعني automatic pipet
+
+25
+00:01:41,150 --> 00:01:45,250
+هلأ automatic pipet هي عبارة عن أداة تستخدم في ال
+
+26
+00:01:45,250 --> 00:01:48,110
+biological و ال biochemical و ال microbiological
+
+27
+00:01:48,110 --> 00:01:52,310
+labs سواء كانت لل teaching purposes أو لل research
+
+28
+00:01:52,310 --> 00:01:56,490
+purposes، تمام؟ يعني تستخدم في مختبرات الأحياء و
+
+29
+00:01:56,490 --> 00:01:59,750
+مختبرات ال biochemistry و ال microbiology في كل
+
+30
+00:01:59,750 --> 00:02:04,700
+أقسام المختبرات الطبيةوتستخدم إما للتدريس أو حتى
+
+31
+00:02:04,700 --> 00:02:09,640
+للعمل لممارسة المهنة أو لأغراض البحث العلمى طبعا
+
+32
+00:02:09,640 --> 00:02:13,700
+من شأنهاي ال bybit أنها تعملى accurate transfer of
+
+33
+00:02:13,700 --> 00:02:18,500
+volume يعني أنها تنقل الحجوم بشكل دقيق جدا بمثاق
+
+34
+00:02:18,500 --> 00:02:22,050
+الكيميا يمكن أخدتوا معامل كيميا قبل هكو كنتوا
+
+35
+00:02:22,050 --> 00:02:25,250
+تستخدموا بايبتات اللي هي زي ال glass pipe اللي كان
+
+36
+00:02:25,250 --> 00:02:29,490
+عبارة عن بايبت glass أداة زجاجية و في آخرها أو في
+
+37
+00:02:29,490 --> 00:02:33,210
+نهايتها بيكون في عندي مستودع من المطاط انا بتحكم
+
+38
+00:02:33,210 --> 00:02:37,160
+بدخول و خروج الهواء و دخول و خروج السائلالى ال by
+
+39
+00:02:37,160 --> 00:02:41,280
+bit طبعا هاي ال glass by bit بتستخدم بال chemical
+
+40
+00:02:41,280 --> 00:02:45,380
+laboratory ولكن للأسف هي not accurate و not
+
+41
+00:02:45,380 --> 00:02:49,820
+precise مش accurate و مش precise متى؟ طبعا لما بدي
+
+42
+00:02:49,820 --> 00:02:53,640
+أنقل حجوم أقل من one ml و احنا هنا بالمختبرات
+
+43
+00:02:53,640 --> 00:02:57,000
+الطبية بنتعامل دائما مع حجوم صغيرة جدا بال
+
+44
+00:02:57,000 --> 00:03:01,970
+microliter يعني أقل من one mlفبالتالي انا بدي أداة
+
+45
+00:03:01,970 --> 00:03:07,230
+تنقل الحجم هذا الصغير او الحجم القليل بطريقة more
+
+46
+00:03:07,230 --> 00:03:11,170
+accurate and more precise how باستخدام ال
+
+47
+00:03:11,170 --> 00:03:15,470
+automatic pipet اذا ال glass pipet هي تستخدم بال
+
+48
+00:03:15,470 --> 00:03:19,670
+chemical laboratory ولكن للأسف في الحجوم الصغيرة
+
+49
+00:03:19,670 --> 00:03:25,060
+not accurate not precise انا محتاجة أداة تكونأكثر
+
+50
+00:03:25,060 --> 00:03:28,560
+دقة more accurate more precise لهيك أنا بستخدم ال
+
+51
+00:03:28,560 --> 00:03:32,200
+automatic by bit لإن كل شغلي بيكون على ال volumes
+
+52
+00:03:32,200 --> 00:03:37,480
+اللي أقل من one ml طبعا طيب كل by bit من هدول ال
+
+53
+00:03:37,480 --> 00:03:42,440
+automatic by bit مصممة إن هي تنقل يعني مدى أو
+
+54
+00:03:42,440 --> 00:03:47,050
+range من الحجوم من إلىيعني في بايبت متكون مصممة
+
+55
+00:03:47,050 --> 00:03:50,930
+تنقل من واحد microliter لعشرة microliter في من ..
+
+56
+00:03:50,930 --> 00:03:54,770
+مصممة بتكون من عشرة microliter لمية microliter في
+
+57
+00:03:54,770 --> 00:03:58,610
+من مية microliter ل one ml تمام ف .. و في من one
+
+58
+00:03:58,610 --> 00:04:04,050
+ml لخمسة ml فإذا ال .. في بايبتات أنواع و حجوم و
+
+59
+00:04:04,050 --> 00:04:08,390
+كل بايبت بتكون إلها range يعني مدى من إلى
+
+60
+00:04:08,390 --> 00:04:15,140
+بتستخدموا في ال .. ال .. ال ..نقل الحجوم هذه و
+
+61
+00:04:15,140 --> 00:04:19,600
+طبعا احنا المفروض احنا مانتخطى هذا ال .. ال .. ال
+
+62
+00:04:19,600 --> 00:04:23,820
+range تبعها يعني لازم انا مثلا اذا بدي انقل خمسين
+
+63
+00:04:23,820 --> 00:04:28,320
+microliter يفضل ان انا اخد بايبت ال range تبعها
+
+64
+00:04:28,320 --> 00:04:31,480
+بحيث انه الخمسين تكون واقعة فيه ال range في داخل
+
+65
+00:04:31,480 --> 00:04:34,980
+هذا ال range يعني مثلا باخد بايبتبتنقل من عشرة
+
+66
+00:04:34,980 --> 00:04:38,420
+microliter لمية microliter ال volume رقم خمسين
+
+67
+00:04:38,420 --> 00:04:42,580
+هيكون already موجود يعني في المدى و ال by بتحتقدر
+
+68
+00:04:42,580 --> 00:04:46,880
+انها تنقلي هذا ال volume فبالتالي انا هكون ضمنت ال
+
+69
+00:04:46,880 --> 00:04:50,250
+accuracy و ال precision لناقل ال volumesطيب إذا
+
+70
+00:04:50,250 --> 00:04:54,850
+إحنا نوّهنا إنه أنا في عندي أنواع وحجوم ويعني قدرة
+
+71
+00:04:54,850 --> 00:04:58,930
+استيعابية لال buy bits يعني مش كلها واحد مش كلها
+
+72
+00:04:58,930 --> 00:05:03,930
+مصممة إنها تنقلني نفس الحجم طيب فإذا في عندي حاجة
+
+73
+00:05:03,930 --> 00:05:08,010
+اسمها buy bit man model طبعا هي سبع أنواع من ال
+
+74
+00:05:08,010 --> 00:05:12,440
+buy bits مصممة إنها تنقل حجوم بطريقة مختلفةطبعا
+
+75
+00:05:12,440 --> 00:05:17,560
+هذه ال .. ال man model يعني لو هم السبعة بيكونوا
+
+76
+00:05:17,560 --> 00:05:20,980
+بينقلولي من point one microliter يعني من اقل من
+
+77
+00:05:20,980 --> 00:05:24,680
+واحد microliter لحد خمس تلاف microliter يعني لحد
+
+78
+00:05:24,680 --> 00:05:30,100
+خمس ML يعني في تنوع من ال by بتات هذه و هلأ بنشوف
+
+79
+00:05:30,100 --> 00:05:35,090
+بالجدوة اللي في ال slide ال ..التالية هيوضحلي إيش
+
+80
+00:05:35,090 --> 00:05:38,970
+يعني by bit مع الموديل ولكن هنا بيحكيلنا إنه the
+
+81
+00:05:38,970 --> 00:05:42,710
+most often used يعني الأكثر استخداما هي ال by
+
+82
+00:05:42,710 --> 00:05:49,150
+bitات اللي تسمى ال B20 و ال B200 و ال B1000 هدول
+
+83
+00:05:49,150 --> 00:05:54,150
+ال models هم الأكثر استخداما اللي بينقلولي حجم من
+
+84
+00:05:54,150 --> 00:05:58,450
+اتنين لعشرينو من خمسين ل متين و من مية ل ألف
+
+85
+00:05:58,450 --> 00:06:02,170
+microliter تمام هاي المدى تبعهم respectively مرتب
+
+86
+00:06:02,170 --> 00:06:07,410
+بالترتيب ال B20 بتنقل من اتنين ل عشرين microliter
+
+87
+00:06:07,410 --> 00:06:12,990
+تمام و ال بايبت B20 بتنقل volume من خمسين ل متين
+
+88
+00:06:12,990 --> 00:06:16,730
+microliter و البايبت الف بتاخد من مية ل الف
+
+89
+00:06:16,730 --> 00:06:20,970
+microliter يعني الرقم اللي جنب ال B تمام بي يعني
+
+90
+00:06:20,970 --> 00:06:25,240
+بايبت و الرقم هذا يعني أقصى قيمة بتقدر تاخدهاطبعا
+
+91
+00:06:25,240 --> 00:06:28,800
+ومش .. مش أدنى قيمة لأ فى أدنى .. الأدنى قيمة غير
+
+92
+00:06:28,800 --> 00:06:32,080
+مذكورة هلأ نيجى نشوف ال buy bet man model هى فى
+
+93
+00:06:32,080 --> 00:06:36,960
+عندى واحد اتنين أربع ستة سبع أنواع من ال buy bet
+
+94
+00:06:36,960 --> 00:06:43,870
+طبعا ومسمى بي تو يعنى بتنقل من zero لتنين طبعاو ال
+
+95
+00:06:43,870 --> 00:06:51,010
+B20 من 0 ل 10 بتاخد ال B20 بتاخد من 0 ل 20 ال B100
+
+96
+00:06:51,010 --> 00:06:55,250
+بتاخد من 0 ل 100 و 200 من 0 ل 200 و هكذا من 0 ل
+
+97
+00:06:55,250 --> 00:07:00,570
+1000 يعني من 1 أمال و هنا من 0 ل 5000 لحد one ..
+
+98
+00:07:00,570 --> 00:07:04,310
+لحد خمس أمال هذا ال range بصي بالهذا اللي بيكون
+
+99
+00:07:04,310 --> 00:07:07,690
+مسجل على ال by بت نفسها يعني بيكون .. بيكون ..
+
+100
+00:07:07,690 --> 00:07:12,960
+بيعطين ال range انه من .. من كذا لكذاأنا لما بدى
+
+101
+00:07:12,960 --> 00:07:16,040
+أستخدم ال by bet هذه طبعا انا مابستخدم ال by bet
+
+102
+00:07:16,040 --> 00:07:18,700
+عشان أنقله zero volume يعني انا مابديش أنقل volume
+
+103
+00:07:18,700 --> 00:07:21,840
+ليش أستخدم ال by bet؟ zero volume يعني no volume
+
+104
+00:07:21,840 --> 00:07:26,160
+طبعا فإذا في عندي حاجة اسمها recommended range
+
+105
+00:07:26,160 --> 00:07:29,600
+يعني ال range الموصفة يعني أنا بستخدم ال by bet
+
+106
+00:07:29,600 --> 00:07:36,260
+لحتى انه انا يعني في range مسموحلي فيه ايش هو اللي
+
+107
+00:07:36,260 --> 00:07:42,040
+مسموحلي فيه؟ من point واحد لاتنين طبعاو ال .. و
+
+108
+00:07:42,040 --> 00:07:45,880
+هنا من نص لعشرة من اتنين لعشرين من عشرة لمية و
+
+109
+00:07:45,880 --> 00:07:50,400
+هكذا من خمسين لمتان طيب هذه ال by bet اللي من
+
+110
+00:07:50,400 --> 00:07:53,780
+خمسين لمتان ممكن تسحب لأقل من خمسين اه ممكن تسحب
+
+111
+00:07:53,780 --> 00:07:56,560
+لأقل من خمسين ال range تبعها اصلا من zero لمتان
+
+112
+00:07:56,900 --> 00:08:00,800
+ولكن هو اذا سحبت اقل من خمسين فهي بتكون inaccurate
+
+113
+00:08:00,800 --> 00:08:05,200
+and imprecise يعني مش accurate ومش precise فانا
+
+114
+00:08:05,200 --> 00:08:08,300
+يفضل ان انا استخ .. اخت .. يعني اتبع ال
+
+115
+00:08:08,300 --> 00:08:13,460
+recommended range علشان أستخ .. أختار ال .. ال ..
+
+116
+00:08:13,460 --> 00:08:18,170
+ضيبة المناسبة لإلي تمام؟طيب يعني مثلا لو أنا بدي
+
+117
+00:08:18,170 --> 00:08:23,030
+أنقل خمسين microliter تمام؟ خمسين microliter مين
+
+118
+00:08:23,030 --> 00:08:29,190
+أفضل أختار ال .. ال by بت مثلا رقم ال B100 ولا ال
+
+119
+00:08:29,190 --> 00:08:33,410
+B200؟ يفضل أن أنا أختار ال B100 لإنه الخمسين
+
+120
+00:08:33,410 --> 00:08:36,570
+القيمة خمسين تقع في نص ال range يعني أنا ب .. بكون
+
+121
+00:08:36,570 --> 00:08:41,710
+ضمنة إنه مافيش أخطاءتمام ال by بت على ال border
+
+122
+00:08:41,710 --> 00:08:45,850
+على الحدود تمام ممكن يكون فيها نسبة خطأ لكن في
+
+123
+00:08:45,850 --> 00:08:49,950
+منتصف ال range بتكون دقيقة فإذا انا بفضل ان انا
+
+124
+00:08:49,950 --> 00:08:54,810
+لما بدي اختار اللي بدي انقل volume بختار ال by بت
+
+125
+00:08:54,810 --> 00:08:57,870
+اللي هذا ال volume يتوسط ال range تبعها او موجود
+
+126
+00:08:57,870 --> 00:09:02,120
+في ال range تبعهاطبعا طيب هلأ لو بدي أنقل مثلا ميت
+
+127
+00:09:02,120 --> 00:09:08,300
+microliter مين أفضل أختار ال B100 طبعا ولا ال B200
+
+128
+00:09:08,300 --> 00:09:15,620
+ولا ال B1000 يفضل أن أنا أختار ال B200 طبعا و ممكن
+
+129
+00:09:15,620 --> 00:09:18,560
+أنا أختار ال B100 بتكون دقيقة يعني ال higher range
+
+130
+00:09:18,560 --> 00:09:23,040
+تبعتها بتكون أدق دايما طبعا ال higher range بتكون
+
+131
+00:09:23,040 --> 00:09:26,680
+أدق من ال lower range لل bybit طبعا يعني هذه ال
+
+132
+00:09:26,680 --> 00:09:32,120
+bybit ال B100يعني لو بدأ أنقل 100 microliter من ال
+
+133
+00:09:32,120 --> 00:09:37,620
+volume ممكن أستخدم ال B200 ال .. ال .. ال B100
+
+134
+00:09:37,620 --> 00:09:43,440
+ممكن أستخدم ال B1000 تمام؟ و .. لكن مثلا هذه ال by
+
+135
+00:09:43,440 --> 00:09:47,500
+bit اللي هو ال range تبعها من عشرة لمية تمام؟ عند
+
+136
+00:09:47,500 --> 00:09:50,800
+المية بتكون أضاق من من .. من .. من أني أسحب فيها
+
+137
+00:09:50,800 --> 00:09:54,640
+عشرة microliter يعني دايما ال higher limit تبعتها
+
+138
+00:09:54,640 --> 00:09:57,990
+بتكون أضاق من ال lower limit لل by bit نفسهاطبعا
+
+139
+00:09:57,990 --> 00:10:02,750
+إذا نرجع نعيد الصياغة تاني لو أنا في عندى .. بدي
+
+140
+00:10:02,750 --> 00:10:05,690
+أنقل 100 microliter مين أفضل أستخدم؟ الأفضل أن أنا
+
+141
+00:10:05,690 --> 00:10:09,990
+أستخدم ال B200 لإنها المية تتوسط ال range ممكن
+
+142
+00:10:09,990 --> 00:10:13,730
+أستخدم ال B100 لإنه حكينا ال higher limit بتكون
+
+143
+00:10:13,730 --> 00:10:16,990
+أدق دايما من ال lower limit لل by bit ولكن أنا
+
+144
+00:10:16,990 --> 00:10:20,110
+مابستخدم .. يعني ممكن أستخدمها بس مابكنش دقيقة و
+
+145
+00:10:20,110 --> 00:10:24,170
+.. و .. كتير لإنه دايما حكينا إنه ال lower limit
+
+146
+00:10:24,170 --> 00:10:27,370
+لل by bit بتكون أقل دقة من ال higher limitهذا
+
+147
+00:10:27,370 --> 00:10:31,690
+بالنسبة ل .. ال .. ال .. ال by bit man model حكينا
+
+148
+00:10:31,690 --> 00:10:36,770
+مين هم اكتر شيوعا و اكتر استخداما بي twenty و ال
+
+149
+00:10:36,770 --> 00:10:41,910
+by one hundred و ال by two hundred تمام؟ اذا ال by
+
+150
+00:10:41,910 --> 00:10:46,570
+عشرين ال by مية و ال by متين هم اكتر ال .. ال by
+
+151
+00:10:46,570 --> 00:10:50,570
+بتات شيوعا و استخداماتمام طيب هلا بنا نحكي عن
+
+152
+00:10:50,570 --> 00:10:56,150
+أنواع .. يعني sorry مكونات البيبت و أجزاقها طيب
+
+153
+00:10:56,150 --> 00:11:00,430
+البيبت زي ما انتوا شايفين بهذا الشكل مكونة من حاجة
+
+154
+00:11:00,430 --> 00:11:05,030
+اسمها ال .. البش .. البشبطن أو البلنجر نحكي عنه
+
+155
+00:11:05,030 --> 00:11:09,770
+إما بشبطن أو بلنجر إيش يعني بشبطن أو بلنجر؟زي ما
+
+156
+00:11:09,770 --> 00:11:13,250
+انتوا شايفين هذا ال push button هو عبارة عن كبسة
+
+157
+00:11:13,250 --> 00:11:18,830
+push انا ممكن انا اضغط عليها و تطلع و تنزل من ال
+
+158
+00:11:18,830 --> 00:11:24,030
+by button تمام؟ و هذا الرد تبعها طبعا هذا الرد ال
+
+159
+00:11:24,030 --> 00:11:29,980
+.. ال .. ال .. ال ..القضيب هذا ممكن انه يلتف دائري
+
+160
+00:11:29,980 --> 00:11:35,500
+علشان يتحكم في ال volume تمام؟ يتحكم في ال volume
+
+161
+00:11:35,500 --> 00:11:39,620
+اللي موجودة على ال digital screen هنا تمام؟ هلأ في
+
+162
+00:11:39,620 --> 00:11:45,240
+هنا adapter موجود هذا يعني علشان انا اقلل من حركة
+
+163
+00:11:45,240 --> 00:11:47,800
+ال vibe يعني ممكن انا اسبط هذا ال adapter على
+
+164
+00:11:47,800 --> 00:11:51,880
+أصبعي .. على أصبعي السبابة وامسكها بشكل انه يكون
+
+165
+00:11:51,880 --> 00:11:58,230
+انا متحكمة فيها ومسكاها بشكل صحيحهذا بسميه tip
+
+166
+00:11:58,230 --> 00:12:04,410
+eject .. tip ejector أو tip eject button تمام؟ هو
+
+167
+00:12:04,410 --> 00:12:09,730
+عبارة عن كبسة بتتخلص من ال tip يعني بتتحرك .. مجرد
+
+168
+00:12:09,730 --> 00:12:14,360
+ما أضغط عليها ال tip اللي هنا بتفكطبعا هذا عبارة
+
+169
+00:12:14,360 --> 00:12:19,020
+عن ال tip ejector و هنا tip holder و زي ما انتوا
+
+170
+00:12:19,020 --> 00:12:23,560
+شايفين هنا في مكان لأ ال handle من هنا بمسكها و
+
+171
+00:12:23,560 --> 00:12:29,220
+هنا الشاشة اللي بتكون موجود عليها القراءة بتكون
+
+172
+00:12:29,220 --> 00:12:35,070
+موجود عليها القراءة تبعتيطيب، هلأ بالنسبة لل .. ال
+
+173
+00:12:35,070 --> 00:12:39,110
+.. بايبت هي بتيجي تمام لحالها هيك و فيه انا عندي
+
+174
+00:12:39,110 --> 00:12:43,370
+حاجة اسمها tips او بايبت tip طبعا هذه ال .. بايبت
+
+175
+00:12:43,370 --> 00:12:48,400
+tip هي عبارة عن قطع بلاستيكيةهذه زي ما انتوا
+
+176
+00:12:48,400 --> 00:12:54,520
+شايفين في الصورة هنا هي عبارة عن قطع بلاستيكية لت
+
+177
+00:12:54,520 --> 00:12:59,980
+.. ت .. يعني بتتركب على ال .. تستخدم لبيبتات و هي
+
+178
+00:12:59,980 --> 00:13:02,960
+تتراوح في الأحجام تبعتها يعني البيبتات الصغيرة
+
+179
+00:13:02,960 --> 00:13:06,900
+بيكون إلها تبات، بيكون إلها تب صغيرة، البيبتات
+
+180
+00:13:06,900 --> 00:13:11,630
+الأكبر بيكون إلها تب أكبر و هكذاهي قطع بلاستيكية و
+
+181
+00:13:11,630 --> 00:13:16,170
+هذه القطع البلاستيكية بستخدمها انا one use ايش
+
+182
+00:13:16,170 --> 00:13:20,090
+يعني one use يعني بستستخدم لمرة واحدة فقط لنقل حجم
+
+183
+00:13:20,090 --> 00:13:24,210
+لمرة واحدة فقط و بعد هيك انا بتلفها بتخلص منها
+
+184
+00:13:24,210 --> 00:13:29,490
+طبعا فإذا مابرجع استخدمها مرة تانية فهذه هي التبات
+
+185
+00:13:29,490 --> 00:13:35,870
+او القطع اللي بتتركب على ال by bit وبيكون الحجم او
+
+186
+00:13:35,870 --> 00:13:39,550
+ال volume داخلها يعني انا السائل اللي انا بنقله
+
+187
+00:13:39,700 --> 00:13:44,480
+بيكون موجود داخل هاي التب وبيتم ال .. ال .. نقله
+
+188
+00:13:44,480 --> 00:13:48,760
+من مكان إلى آخر حسب أنا وين بدي أو كيف بدي أشتغل
+
+189
+00:13:48,760 --> 00:13:55,780
+طيب، إذا هاي مكونات البايبت و أجزاقها و يعرفنا إنه
+
+190
+00:13:55,780 --> 00:14:00,250
+التب هي قطعة خارجيةتتركب لل by bet مش ثابتة في ال
+
+191
+00:14:00,250 --> 00:14:05,490
+by bet و انه هذه القطعة ايضا ممكن اني استخدمها
+
+192
+00:14:05,490 --> 00:14:09,210
+لمرة واحدة فقط يعني بتلفها بعد ما استخدمها خلاص
+
+193
+00:14:09,210 --> 00:14:13,430
+برميها و اذا هذه يطلق عليها مصطلح disposable
+
+194
+00:14:13,430 --> 00:14:19,040
+disposable يعني استخدام لمرة واحدة فقطوحكينا ايضا
+
+195
+00:14:19,040 --> 00:14:24,320
+انه هذه ال .. ال .. التب يعني بتتنقل .. بيكون
+
+196
+00:14:24,320 --> 00:14:27,980
+حجمها متناسب مع ال bybit ال by بتاعة اللي بتنقل
+
+197
+00:14:27,980 --> 00:14:31,780
+حجوم صغيرة بيكون لها تب صغيرة و ال by بتاعة اللي
+
+198
+00:14:31,780 --> 00:14:37,440
+بتنقل حجم أكبر بيكون لها تب حجمه أكبر نوعا ماطيب
+
+199
+00:14:37,440 --> 00:14:40,980
+فيه هنا نصائح لاستخدام ال micro bibit اللي هي
+
+200
+00:14:40,980 --> 00:14:45,120
+عبارة عن إيش هذه النصائح؟ أول حاجة never exceed
+
+201
+00:14:45,120 --> 00:14:49,140
+the upper or lower limit of this bibit يعني أنه
+
+202
+00:14:49,140 --> 00:14:55,710
+يعني مثلا هاي ال bibit هايبتنقللي مثلا من عشرة
+
+203
+00:14:55,710 --> 00:15:00,350
+لمية، يعني ممنوع أنا أسحب أقل من عشرة و ممنوع أسحب
+
+204
+00:15:00,350 --> 00:15:04,590
+فيها أكتر من مية، يعني ما أسحب أقل من عشرة ولا
+
+205
+00:15:04,590 --> 00:15:09,350
+أكتر من مية، يعني ما أتخطأ ال range هادا، إما بأقل
+
+206
+00:15:09,350 --> 00:15:13,840
+منه أو بأكتر منههذا معناه الجملة هاى الجملة هاى
+
+207
+00:15:13,840 --> 00:15:18,300
+معناها انه never exceed ما اتخطى ال upper او ال
+
+208
+00:15:18,300 --> 00:15:22,320
+lower limit لل range لل by bit ال by bit يعني
+
+209
+00:15:22,320 --> 00:15:28,180
+مصممة بأنها تنقلي داخل range معين فانا باحترم هذا
+
+210
+00:15:28,180 --> 00:15:32,880
+ال range بلتزم فيه ما بستخدمها ال by bit الاقل منه
+
+211
+00:15:32,880 --> 00:15:37,900
+ولا الاكثر منه على نقل حجوم علشان ما اكون بنقل
+
+212
+00:15:37,900 --> 00:15:42,840
+حجوم بطريقة مش precise و مش accurateتمام طيب so
+
+213
+00:15:42,840 --> 00:15:46,840
+the desired volume by turning centrally located
+
+214
+00:15:46,840 --> 00:15:49,700
+ring clockwise to increase volume or
+
+215
+00:15:49,700 --> 00:15:53,120
+counterclockwise to decrease احنا حكينا انه هذا
+
+216
+00:15:53,120 --> 00:15:59,290
+القضيب او ال .. ال .. ال ..الرد تمام هذا بيتحرك
+
+217
+00:15:59,290 --> 00:16:02,530
+بشكل دائري و من خلاله انا بتحكم بالحجم اللى موجود
+
+218
+00:16:02,530 --> 00:16:06,290
+على الشاشة هنا تمام يعني من خلاله انا بحدد الحجم
+
+219
+00:16:06,290 --> 00:16:11,450
+اللى بديها بحكيلك هنا انه تحركيه ال ال ال
+
+220
+00:16:11,450 --> 00:16:18,530
+clockwise تمام و عشان يزيد الحجم و بنحركه counter
+
+221
+00:16:18,530 --> 00:16:25,520
+clockwise to decrease ال volumeطبعا انا هنا بالشكل
+
+222
+00:16:25,520 --> 00:16:29,140
+او بالتجربة العملية لما بنشرح احنا كيف نستخدم ال
+
+223
+00:16:29,140 --> 00:16:32,720
+bybit في المعامل انا بخل ال bybit .. بخل الطالبة
+
+224
+00:16:32,720 --> 00:16:37,120
+تمسك ال bybit تمام و بحيث انه ال digital screen
+
+225
+00:16:37,120 --> 00:16:41,300
+تكون مواجهةلإلها تمام يعني تكون عينها على ال
+
+226
+00:16:41,300 --> 00:16:45,800
+digital screen وبالتالي بما أنه بيكون عينها على ال
+
+227
+00:16:45,800 --> 00:16:49,660
+digital screen إذا حركت هذا الرد بشكل دائري
+
+228
+00:16:49,660 --> 00:16:54,260
+باتجاهها يعني لعندها تمام هيك بيزيد ال volume أما
+
+229
+00:16:54,260 --> 00:16:59,180
+إذا حركته باتجاه المعاكس يعني بعيد عنها فهو بيقل
+
+230
+00:16:59,180 --> 00:17:02,340
+ال volume تمام هذا بده ممارسة ان شاء الله بنشرحه
+
+231
+00:17:02,340 --> 00:17:09,020
+بالطريقة أو بالتجربة العمليةتمام؟ هذا بالنسبة ل ..
+
+232
+00:17:09,020 --> 00:17:14,320
+ال .. ال .. ال by bet و الطرق أو اللي هي الخطوات
+
+233
+00:17:14,320 --> 00:17:17,740
+أو اللي .. ال .. التعليمات اللي لازم أنا ألتزم
+
+234
+00:17:17,740 --> 00:17:22,920
+فيها أثناء السحب أو نقل الحجوم باستخدام ال by bet
+
+235
+00:17:23,850 --> 00:17:29,330
+في شغل هنا انه ال .. ال by bit هنا طبعا هي بتكون
+
+236
+00:17:29,330 --> 00:17:33,210
+مصممة انها تنقلي او تقفز قفزات في ال volume يعني
+
+237
+00:17:33,210 --> 00:17:38,970
+مثلا هاي هنا بحكيلي انه هي صح يعني من خمسين ل متين
+
+238
+00:17:38,970 --> 00:17:45,210
+بتسحب لكن انه مثلا انا لما بدي ..أحرك بصير بحرك
+
+239
+00:17:45,210 --> 00:17:48,450
+بيزيد ال volume كل شوية point اتنين point اتنين
+
+240
+00:17:48,450 --> 00:17:51,890
+لحد ما اصل لل volume اللي بديها لذا هذه هي ال ..
+
+241
+00:17:51,890 --> 00:17:55,450
+ال .. مدى التحريك او اللي هي القافزات في ال
+
+242
+00:17:55,450 --> 00:18:01,330
+volumes طبعا طيب بليت ا tip place a tip on the
+
+243
+00:18:01,330 --> 00:18:05,230
+discharge end of the pipet طبعا انا ب .. بركب ال
+
+244
+00:18:05,230 --> 00:18:10,370
+tip في المكان المخصص لإلها و بعد هيك طبعا ب ..
+
+245
+00:18:12,600 --> 00:18:16,600
+باستخدام البلانجر ب .. باستخدمه علشان اسحب ال
+
+246
+00:18:16,600 --> 00:18:20,520
+volumes هذا البلانجر اللي احنا شايفينه تمام مش ما
+
+247
+00:18:20,520 --> 00:18:24,180
+.. ايضا هو يعني فايد .. فايديته مش بس انه متصل
+
+248
+00:18:24,180 --> 00:18:28,360
+بالرد high بال .. و بتحكم بال volume لأ هذا
+
+249
+00:18:28,360 --> 00:18:31,600
+البلانجر احنا كتركم push button يعني انا ممكن اكدس
+
+250
+00:18:31,600 --> 00:18:35,480
+عليا بيطلع و بينزلطبعا انا هذا لما بدي أكبس عليه و
+
+251
+00:18:35,480 --> 00:18:39,860
+يطلع و ينزل بينزل عند ضغطتين، الضغطة الأولى لما
+
+252
+00:18:39,860 --> 00:18:43,840
+أضغط الضغطة الأولى بيقللي ال exact volume اللي
+
+253
+00:18:43,840 --> 00:18:50,720
+موجود ع الشاشة تمام؟ لما أضغط ضغطتين تمام؟بينقلي
+
+254
+00:18:50,720 --> 00:18:54,280
+extra volume ممكن يكون حجم مضاعف او extra volume
+
+255
+00:18:54,280 --> 00:18:59,560
+من ال volume اللي مصمم او موجود على الشاشة تمام؟
+
+256
+00:18:59,560 --> 00:19:04,400
+اذا هذا ال bush هذا البلنجر عبارة عن كبسة فيها two
+
+257
+00:19:04,400 --> 00:19:07,680
+stops يعني انا لما اضغط اول ضغطة بلاحظ انه خلاص
+
+258
+00:19:07,680 --> 00:19:12,780
+وجه اصبعي لدرجة معينة وانا بضغط لو ضغطت بقوة اكبر
+
+259
+00:19:12,780 --> 00:19:16,910
+ممكن اضغط الضغطة التانية يعني بنزل كمان مرةفالضغطة
+
+260
+00:19:16,910 --> 00:19:21,410
+الأولى لما أنا بكون ضغطاها بتكون ال by بتها تسحبلي
+
+261
+00:19:21,410 --> 00:19:25,750
+volume نفس ال volume اللى موجودة ع الشاشة لكن لو
+
+262
+00:19:25,750 --> 00:19:29,690
+أنا بدى أسحب two volumes او يعني بصري بدى أسحب و
+
+263
+00:19:29,690 --> 00:19:33,110
+أنا ضغطة ضغطتين هيسحبلي ال volume اللى موجودة ع
+
+264
+00:19:33,110 --> 00:19:37,610
+الشاشة وكمان extra volume تانى يعني volume بزيادة
+
+265
+00:19:37,610 --> 00:19:40,710
+طب انا ليش امتى بستخدم الضغطة الأولى و امتى بستخدم
+
+266
+00:19:40,710 --> 00:19:45,100
+الضغطة التانية او الضغطتين هلأ بنشوف كدههي هنا
+
+267
+00:19:45,100 --> 00:19:47,920
+بيحكيلي انه البلانجر او اللي هو ال push button
+
+268
+00:19:47,920 --> 00:19:53,140
+تمام و ال stop at two different positions يعني هذا
+
+269
+00:19:53,140 --> 00:19:57,740
+البلانجر بيتوقف عند .. عند مكانين و انا لما اجي
+
+270
+00:19:57,740 --> 00:20:00,820
+اضغط عليه بلاحظ انه في عندي two resistance في
+
+271
+00:20:00,820 --> 00:20:05,020
+مقاومتين للضغطة تبعتي ال .. ال stop الأول او
+
+272
+00:20:05,020 --> 00:20:10,160
+الضغطة الأولى تمام و لو انا زودت ال .. الضغط تبعي
+
+273
+00:20:10,160 --> 00:20:16,630
+بنزل كمان ضغطة تانية فبالتاليال .. ال .. ال first
+
+274
+00:20:16,630 --> 00:20:21,870
+stopping تمام عند ال initial resistant تمام عند
+
+275
+00:20:21,870 --> 00:20:27,610
+أول يعني stop عند أول إعاقة لضغطة تبعتي بيجف ال ..
+
+276
+00:20:27,610 --> 00:20:30,850
+ال plunger عندها بتكون هذه عبارة عن الضغطة الأولى
+
+277
+00:20:31,230 --> 00:20:37,590
+طبعا و ال .. و هذه معناها انه انا هسحب ايش ال
+
+278
+00:20:37,590 --> 00:20:40,810
+desired volume يعني انا هاخد ال volume اللي موجود
+
+279
+00:20:40,810 --> 00:20:47,720
+ع الشاشة exactly باخده طبعا ال second stoppingلما
+
+280
+00:20:47,720 --> 00:20:53,380
+يكون .. لما انا بدي اضغط على الضغط التاني يعني انا
+
+281
+00:20:53,380 --> 00:20:58,900
+بدي اظل نازلة في البلانجر ضغطين فساعتها انا بكون
+
+282
+00:20:58,900 --> 00:21:02,540
+تجاوزت ال initial resistance عند .. تجاوزت الإعاقة
+
+283
+00:21:02,540 --> 00:21:06,540
+الأولى و انزلت كمان مرة في ال .. في البلانجرطبعا
+
+284
+00:21:06,540 --> 00:21:12,600
+فهدى ال .. الاشي معناها انه ال by bit راح تسحبلي
+
+285
+00:21:12,600 --> 00:21:17,940
+volume extra طبعا هتسحبلي ال .. ال volume extra
+
+286
+00:21:17,940 --> 00:21:22,720
+ساعتها انا لما بلقل الحجم بفضيه بضغط ضغطة واحدة
+
+287
+00:21:22,950 --> 00:21:26,350
+هتنقل الحجم و هيضل في عندي extra volume موجود في
+
+288
+00:21:26,350 --> 00:21:31,130
+ال tip ممكن أتخلص منه تمام؟ اذا في عندي ال .. ال
+
+289
+00:21:31,130 --> 00:21:34,370
+.. بلانجر ال .. او ال .. ال push button بيجف عن
+
+290
+00:21:34,370 --> 00:21:38,430
+ضغطين، الدغطة الأولى بتن .. لما انا أكون ضغطاها و
+
+291
+00:21:38,430 --> 00:21:42,270
+أسحب حجم من السائل معناها أنا سحبت نفس الحجم اللي
+
+292
+00:21:42,270 --> 00:21:45,600
+موجودة على الشاشةلكن اذا انا كنت ضغطة ضغطتين و
+
+293
+00:21:45,600 --> 00:21:50,120
+سحبت حجم معناها انا سحبت الحجم مع extra تمام لما
+
+294
+00:21:50,120 --> 00:21:54,040
+اجي افضية بدي انتبه ان انا اضغط ضغطة واحدة فقط
+
+295
+00:21:54,040 --> 00:21:57,520
+افضي ال volume اللي من ضغطة واحدة لان ضغطة واحدة
+
+296
+00:21:57,520 --> 00:22:00,380
+يعني ايش ال desired volume يعني ال volume اللي
+
+297
+00:22:00,380 --> 00:22:04,580
+بديها و بيضل في عندي extra طبعا طب ليش الغلبة هذه
+
+298
+00:22:04,580 --> 00:22:07,880
+ليش ضغطة واحدة و ضغطتين و ليش انا اخد extra volume
+
+299
+00:22:08,030 --> 00:22:11,550
+علشان في عندي نوعين من ال by betting نوع اسمه
+
+300
+00:22:11,550 --> 00:22:16,450
+forward by betting و reverse by betting ايش يعني
+
+301
+00:22:16,450 --> 00:22:19,770
+forward by betting يعني انه انا افوت في المحلول
+
+302
+00:22:19,770 --> 00:22:23,870
+تمام و انا ضغطة ضغطة واحدة اخد الحجم اللي بدياه هو
+
+303
+00:22:23,870 --> 00:22:27,390
+نفس الحجم اللي موجود على الشاشة exactly بالضبط
+
+304
+00:22:27,390 --> 00:22:32,530
+بدون زيادة و بعد هيك اجي افضيه افضيه في المحلول في
+
+305
+00:22:32,530 --> 00:22:37,350
+الوعاء اللي انا بدي اشتغل فيه و بفضيه و بضل ضغطة
+
+306
+00:22:37,540 --> 00:22:40,020
+ممكن اضغط اكتر من مرة او ممكن اضغط اكتر من مرة او
+
+307
+00:22:40,020 --> 00:22:44,880
+ممكن اضغط اكتر من مرة او ممكن اضغط اكتر من مرة او
+
+308
+00:22:44,880 --> 00:22:48,820
+ممكن اضغط اكتر من مرة او ممكن اضغط اكتر من مرة او
+
+309
+00:22:48,820 --> 00:22:48,960
+ممكن اضغط اكتر من مرة او ممكن اضغط اكتر من مرة او
+
+310
+00:22:48,960 --> 00:22:49,180
+ممكن اضغط اكتر من مرة او ممكن اضغط اكتر من مرة او
+
+311
+00:22:49,180 --> 00:22:49,860
+ممكن اضغط اكتر من مرة او ممكن اضغط اكتر من مرة او
+
+312
+00:22:49,860 --> 00:22:52,000
+ممكن اضغط اكتر من مرة او ممكن اضغط اكتر من مرة او
+
+313
+00:22:52,000 --> 00:22:53,780
+ممكن اضغط اكتر من مرة اإذا إيش ال forward
+
+314
+00:22:53,780 --> 00:22:57,380
+bybiting؟ معناها .. معناها إن أنا أضغط ضغطة على ال
+
+315
+00:22:57,380 --> 00:23:01,680
+bybit بعدين أجي أس .. أ .. أنقل الحجم أاخد الحجم
+
+316
+00:23:01,680 --> 00:23:04,200
+اللي بدياه هو ال exact volume اللي موجود على
+
+317
+00:23:04,200 --> 00:23:08,120
+الشاشة و لما أجي أفضيه في الوعاء ممكن أضغط ضغط أو
+
+318
+00:23:08,120 --> 00:23:11,040
+ضغطتين عشان أتأكد إن أنا فضيته كله و إن أنا
+
+319
+00:23:11,040 --> 00:23:14,700
+استخدمت ال volume بالضبط هو نفس اللي موجود على
+
+320
+00:23:14,700 --> 00:23:17,720
+الشاشة تمام؟ مايكونش ضاي .. إيش يضاي العالق في ال
+
+321
+00:23:17,720 --> 00:23:23,070
+tip تمام؟ إذا ..هذا معناه ال forward وهذه الطريقة
+
+322
+00:23:23,070 --> 00:23:27,410
+تستخدم عشان انا لو بدى انقل حجوم زى مين زى ال
+
+323
+00:23:27,410 --> 00:23:31,450
+aqueous solution المحليل اللى أصلها مية ال buffers
+
+324
+00:23:31,450 --> 00:23:35,310
+ال acid ال bases كل الأشياء المحليل هذه اللى أصلها
+
+325
+00:23:35,310 --> 00:23:39,750
+مية بستخدم معاها طريقة ال forward by bettingأما
+
+326
+00:23:39,750 --> 00:23:43,390
+طريقة ال reverse by betting فهي عبارة عن انه انا
+
+327
+00:23:43,390 --> 00:23:47,990
+باخد larger volume of solution بصحب larger volume
+
+328
+00:23:47,990 --> 00:23:51,990
+of solution ايش يعني؟ يعني بصحب extra يعني معناها
+
+329
+00:23:51,990 --> 00:23:55,430
+انا لما باجي باخد ال by bet او بنقل في ال by bet
+
+330
+00:23:55,430 --> 00:24:00,870
+بكون ضغطة، ضغطتين تمام؟ باخد الحجم اللي بديها مع
+
+331
+00:24:00,870 --> 00:24:05,750
+extra تمام؟و لما اجى افضى فى المحلول بتغضى تواحدة
+
+332
+00:24:05,750 --> 00:24:11,030
+بحيث ان انا افضى فقط المحلول الحجم اللى بديه و يضل
+
+333
+00:24:11,030 --> 00:24:14,330
+ال extra اللى موجود فى التب اتخلص منه طب انا ليش
+
+334
+00:24:14,330 --> 00:24:20,430
+يعني بلجأ لهذه الطريقة بحكيلي لإنه فى سوائل عبارة
+
+335
+00:24:20,430 --> 00:24:25,250
+عن لازجة viscous طبعا هاد اش يعني viscous لازجة
+
+336
+00:24:25,250 --> 00:24:29,130
+يعني بصعوبة هتطلع جوا التب المحليل بصعوبة هتطلع
+
+337
+00:24:29,130 --> 00:24:35,520
+جوا التب طبعا طيبفبالتالي لما اجي انا اكون ماخدة
+
+338
+00:24:35,520 --> 00:24:39,200
+ال volume وزيادة شوية هكون ضامنة ان انا اخدت ال
+
+339
+00:24:39,200 --> 00:24:42,060
+volume ..يعني ضامنة ان انا هنزل ال volume على
+
+340
+00:24:42,060 --> 00:24:47,480
+الأقل اللي بديها، تمام؟ لكن مثلا لو انا ده استخدمت
+
+341
+00:24:47,480 --> 00:24:52,430
+مع الهادي المحلي للviscousاستخدمت نظامة ضغطة لوحدة
+
+342
+00:24:52,430 --> 00:24:57,050
+ما هي بتطلع جوا التب بطء يعني انا ممكن افوت و اطلع
+
+343
+00:24:57,050 --> 00:25:01,590
+و اخد ال باقي الحجم اللي بدياه بس لسه السائل من
+
+344
+00:25:01,590 --> 00:25:08,200
+كتر لزوجته ماعباش جوا التب بشكلكامل فبالتالي ممكن
+
+345
+00:25:08,200 --> 00:25:12,800
+اخد volume اقل من اللي انا بديها فهنا ممكن يكون في
+
+346
+00:25:12,800 --> 00:25:17,120
+خطأ عشان هيك بحكيلي انه استخدمي مع السوائل اللازجة
+
+347
+00:25:17,120 --> 00:25:21,200
+انه طريقة انك انت تاخدي بزيادة و لما تفض تفضي
+
+348
+00:25:21,200 --> 00:25:27,230
+الحجم اللي بدكياتمام هذا بالنسبة لمين ل .. ال ..
+
+349
+00:25:27,230 --> 00:25:31,150
+ال .. السواقل اللازجة بستخدم معاها ال reverse
+
+350
+00:25:31,150 --> 00:25:34,330
+technique يعني نظام الضغطين عشان أخد extra volume
+
+351
+00:25:34,330 --> 00:25:37,590
+أيضا بيحكيلي فيه محاليل volatile إيش يعني
+
+352
+00:25:37,590 --> 00:25:42,030
+volatile؟ يعني متطايرة يعني مجرد مثلا ما أسحبها في
+
+353
+00:25:42,030 --> 00:25:46,350
+ال pipe و أنقلها لل .. للوعائل عشان أفضيها ممكن
+
+354
+00:25:46,350 --> 00:25:50,810
+تكون اتبخرت فهنا بيصير في عندي فاقد في الحجم
+
+355
+00:25:50,810 --> 00:25:58,290
+فبالتالي أنا بكون نقلت حجمأقل من اللازم فهنا باع
+
+356
+00:25:58,290 --> 00:26:05,490
+هذه النوع من السوائل ايضا ممكن انه استخدم نظام
+
+357
+00:26:05,490 --> 00:26:09,230
+الضغطين بحيث ان انا اخد حجم بزيادة و لما افضل افضل
+
+358
+00:26:09,230 --> 00:26:14,030
+exact volume عشان اذا مهما يكون الطاير من هذا ال
+
+359
+00:26:14,030 --> 00:26:18,050
+solution اكون على الأقل مازلة محتفظة بال volume
+
+360
+00:26:18,050 --> 00:26:21,890
+اللي انا بديهاتمام؟ أيضا في بعض المحاليل اللي
+
+361
+00:26:21,890 --> 00:26:24,510
+بنسميها foaming solution ايش يعني foaming
+
+362
+00:26:24,510 --> 00:26:28,950
+solution؟ يعني محاليل بتعمل رغبة تمام؟ فيها مادة
+
+363
+00:26:28,950 --> 00:26:33,190
+صابون او يعني مادة ..الها ملمة الصابونة هذه
+
+364
+00:26:33,190 --> 00:26:37,690
+المحاليل اللي فيها مواد ذات ملمة الصابونة تمام؟
+
+365
+00:26:37,690 --> 00:26:41,120
+ممكن تعملي رغبةو بالتالي لما أجي أصحب أصحب من
+
+366
+00:26:41,120 --> 00:26:45,220
+الرغبة high فبالتالي أكون نقلت volume أقل من
+
+367
+00:26:45,220 --> 00:26:49,520
+اللازم لإن أنا يعني جزء من الرغبة احتلت ال tub
+
+368
+00:26:49,520 --> 00:26:53,300
+تمام؟ و أفكر حالي إن أنا أخدت الحجم اللي بدياه و
+
+369
+00:26:53,300 --> 00:26:59,700
+فعليا أنا بكون ماخدة حجم أقل من ال ..المفروض
+
+370
+00:26:59,700 --> 00:27:03,300
+فبالتالي بستخدم برضه نظام ال larger volume of
+
+371
+00:27:03,300 --> 00:27:06,120
+solution يعني ان انا بدي اخد ال extra volume يعني
+
+372
+00:27:06,120 --> 00:27:09,700
+بدي استخدم ال reverse technique يعني نظام ضغطتين و
+
+373
+00:27:09,700 --> 00:27:17,300
+.. و بعد هيك شسمه بروح بفضي بضغطة واحدةتمام؟ عشان
+
+374
+00:27:17,300 --> 00:27:21,780
+لو كان في رغبة في المحلول أكون ضامنة أنها ما تأثر
+
+375
+00:27:21,780 --> 00:27:25,360
+على الحجم اللي أنا بدي أنقله تمام؟ و أيضا عشان لو
+
+376
+00:27:25,360 --> 00:27:29,180
+أنا بدي أنقل حجوم صغيرة جدا جدا يعني أنا خايفة ..
+
+377
+00:27:29,180 --> 00:27:32,260
+يعني مش متأكدة أخدت ال volume ولا لأ لأنه في حجوم
+
+378
+00:27:32,260 --> 00:27:35,600
+يعني زي مثلا حجم one microliterيعني انت حتى ما
+
+379
+00:27:35,600 --> 00:27:39,040
+بتشوفيه على التب يعني صعب تشوفيه على التب انه انت
+
+380
+00:27:39,040 --> 00:27:42,720
+أخدتي او ما أخدتي فالأفضل ان انا ااخد حجم بزيادة و
+
+381
+00:27:42,720 --> 00:27:45,560
+لما افضي داخل ال solution او المقلوعه اللي انا بدي
+
+382
+00:27:45,560 --> 00:27:50,640
+اشتغل فيه بفضي بالحجم اللي بديها اذا .. اذا لما
+
+383
+00:27:50,640 --> 00:27:56,550
+انا اسحب الحجمبضغطة و افضيه بدغطتين معناها انا بدي
+
+384
+00:27:56,550 --> 00:28:01,050
+اخدت exact volume و افضيته بدغطتين عشان اضمن ان
+
+385
+00:28:01,050 --> 00:28:05,270
+انا افضيته كويس تمام هذه طريقة forward bibuting
+
+386
+00:28:05,270 --> 00:28:10,510
+لكن لما انا اسحب الحجم بدغطتين و افضيه بدغطة واحدة
+
+387
+00:28:10,510 --> 00:28:14,590
+معناها انا استخدمت طريقة ال reverse bibuting و هذا
+
+388
+00:28:14,590 --> 00:28:20,490
+طبعا بيختلفهلأ ال .. ال .. اذا هذه ال bybit شايفين
+
+389
+00:28:20,490 --> 00:28:24,570
+هي البلانجر بالكبس عليه في عندى ضغطة واحدة او
+
+390
+00:28:24,570 --> 00:28:28,690
+ضغطتان depress the blanger until you feel the
+
+391
+00:28:28,690 --> 00:28:32,090
+initial resistance مجرد ما انت تضغطي طبعا ان شاء
+
+392
+00:28:32,090 --> 00:28:35,670
+الله راح نرجع على المعامل و امسكهم ال bybit كل بنت
+
+393
+00:28:35,670 --> 00:28:39,770
+راح يكونيلها bybitو تشوف و تجرب كيف تشتغل عليها
+
+394
+00:28:39,770 --> 00:28:43,130
+كيف تضغط .. كيف لما بتضغط بتقابل ال resistant
+
+395
+00:28:43,130 --> 00:28:46,750
+الأولى اللي هي الإعاقة الأولى يعني إشي بيعيط مصاري
+
+396
+00:28:46,750 --> 00:28:50,590
+ال blanket إشي بيوقفه هذه بتكون الدغطة الأولى و
+
+397
+00:28:50,590 --> 00:28:55,390
+بقى لو .. لو كبست بقوة أكبر بتكون ساعتها أخدت ال
+
+398
+00:28:55,390 --> 00:28:59,730
+.. ال .. ال vibe أخدت الدغطتين تمام فيعني بصير ده
+
+399
+00:28:59,730 --> 00:29:05,530
+تسحب extra volumeطيب فبالتالي لما احنا بدنا نفوت
+
+400
+00:29:05,530 --> 00:29:09,090
+في ال .. ال .. ال vibe طبعا لما اجي افوتها بفوتها
+
+401
+00:29:09,090 --> 00:29:13,190
+بحيث انها تكون مغموقة جزء منها مغمور او جزء من
+
+402
+00:29:13,190 --> 00:29:18,330
+التب مغمور داخل ال solution تمام؟ مابروح اخلي
+
+403
+00:29:18,330 --> 00:29:23,290
+الفتحة التب او هنا يعني الفوهة التب ملامسة للسطح
+
+404
+00:29:23,290 --> 00:29:26,450
+لإنه انا ممكن اسحب سائل و هوا مع بعض مدام ها على
+
+405
+00:29:26,450 --> 00:29:31,690
+السطحو أيضا كمان ما بخليها تلامس البطم لل solution
+
+406
+00:29:31,690 --> 00:29:36,630
+ليش؟ لإن هذه الفوهة أو فتحة التب ممكن يغلقها اللي
+
+407
+00:29:36,630 --> 00:29:43,810
+هي قاع ال container او ال tube فبالتالي انه انا
+
+408
+00:29:43,810 --> 00:29:48,550
+بكون هي كعملت انسداد للتب و مش راح اسحب ال volume
+
+409
+00:29:48,550 --> 00:29:52,730
+اللي بدي يعني تمام؟ اذا بضغطال .. الضغطة أولة أو
+
+410
+00:29:52,730 --> 00:29:57,510
+ضغطة تانية حسب نوع ال .. ال .. ال bybiting لو أنا
+
+411
+00:29:57,510 --> 00:30:01,890
+بستخدم forward bybiting هضغط ضغطة واحدة تمام بفوت
+
+412
+00:30:01,890 --> 00:30:04,830
+ال tip بحيث أنه يكون جزء منها مغمور داخل ال
+
+413
+00:30:04,830 --> 00:30:08,050
+solution مش على السطح ولا على .. في القاع م ..
+
+414
+00:30:08,050 --> 00:30:12,630
+ملامسة للقاع و ببدأ بعد هيك إيش أعمل أرفع إيدي
+
+415
+00:30:12,630 --> 00:30:18,350
+بالتدريج عن البلانجر بالتدريج تمام مهم جدا أن أنا
+
+416
+00:30:18,350 --> 00:30:22,860
+أرفع إيدي بالتدريج عن البلانجرمارفعش هيك فجأة
+
+417
+00:30:22,860 --> 00:30:28,380
+واحدة ليش؟ لإن لو رفعت فجأة راح يندفع ال solution
+
+418
+00:30:28,380 --> 00:30:33,520
+بقوة داخل ال .. ال .. التب فهذا هيسببلي أخطاء ممكن
+
+419
+00:30:33,520 --> 00:30:37,340
+ما أخد ال volume اللي بديه ممكن ال .. ال .. السائل
+
+420
+00:30:37,340 --> 00:30:40,940
+يندفع ويفوت داخل ال pipe ذات نفسها تمام؟ يعني يطلع
+
+421
+00:30:40,940 --> 00:30:44,560
+من التب ويفوت داخل ال pipe ويقدي إلى الصدق ل ..
+
+422
+00:30:44,560 --> 00:30:49,380
+لإلها أو إلى التلف تمام؟ وممكن أكون يعني يسحب هوا
+
+423
+00:30:49,750 --> 00:30:52,750
+معاها يعني يكون فيه فقاعات فبالتالي يكون فيه error
+
+424
+00:30:52,750 --> 00:30:56,830
+في ال volume اللي نقلته فبالتالي يفضل انه انا لما
+
+425
+00:30:56,830 --> 00:31:00,910
+اجي ارفع البلانجر ارفعه بالتدريج عشان المحلول يفوت
+
+426
+00:31:00,910 --> 00:31:05,710
+بالتدريج او ببطء داخل ال pipet ويفوت بشكل دقيق و
+
+427
+00:31:05,710 --> 00:31:11,220
+اكون انا عم باخد الحجم الدقيق تمام؟بعد هيك بروح
+
+428
+00:31:11,220 --> 00:31:15,120
+على ال container التاني و ب .. أضغط على البلانجر
+
+429
+00:31:15,120 --> 00:31:19,720
+مرتين يعني على ال initials ال resistant و بفضيه و
+
+430
+00:31:19,720 --> 00:31:22,320
+ممكن أضغط على ال second resistant عشان أتأكد أن
+
+431
+00:31:22,320 --> 00:31:26,040
+أنا فضيت كل ال volume و بعد هيك بتخلص من ال tip
+
+432
+00:31:26,040 --> 00:31:29,080
+كيف بتخلص من ال tip؟ حكينا أنه في هنا على الطرف
+
+433
+00:31:29,080 --> 00:31:33,720
+حاجة اسمها ejector لل tipهنا مجرد ما انا اضغط على
+
+434
+00:31:33,720 --> 00:31:37,500
+هذا ال ejector لل tip ال tip بتكون خلاص فانفصلت عن
+
+435
+00:31:37,500 --> 00:31:43,100
+ال pipet و انا بتخلص منها فلما بدي اتخلص من ال tip
+
+436
+00:31:43,100 --> 00:31:47,520
+بوجه ال pipet هذه باتجاه السلة تمام و بضغط على ال
+
+437
+00:31:47,520 --> 00:31:53,860
+ejector فلحالها ال pipet بتروح على الفضلات
+
+438
+00:31:54,650 --> 00:31:59,550
+ع الوسط طبعا هذا بالنسبة ل .. ال .. ال forward
+
+439
+00:31:59,550 --> 00:32:05,230
+بالنسبة ل reverse طبعا يعني تقريبا نفس التعليمات
+
+440
+00:32:05,230 --> 00:32:08,270
+ولكن انه انا لما بدي افوت ضغطة بدي اكون ضغطة
+
+441
+00:32:08,270 --> 00:32:13,250
+ضغطتين و بدي افوت ب .. و اكون جزء من الطب مغمور
+
+442
+00:32:13,250 --> 00:32:17,510
+داخل ال solution طبعا بفوت بشكل عمودي يا بنات لو
+
+443
+00:32:17,510 --> 00:32:20,710
+انا بدي افوت بشكل مائل بيأثر على الحجم اللي انا
+
+444
+00:32:20,710 --> 00:32:24,510
+مخدعةبفوت بشكل عمودي داخل ال solution جزء من ال
+
+445
+00:32:24,510 --> 00:32:29,230
+tip مغمور داخل ال solution و برفع ايدي بالتدريج عن
+
+446
+00:32:29,230 --> 00:32:32,670
+البلنجر بسحبلي ال volume طبعا هنا في ال reverse
+
+447
+00:32:32,670 --> 00:32:37,190
+انا ضغطت ضغطتين فهيسحبلي extra volume تمام؟بعدين
+
+448
+00:32:37,190 --> 00:32:40,570
+بروح على ال container اللي انا بدي افض فيه و بضغط
+
+449
+00:32:40,570 --> 00:32:44,230
+ضغطة واحدة علشان تنزلي ال solution اللي انا بديها
+
+450
+00:32:44,230 --> 00:32:48,210
+فقط الحجم اللي بديها فقط و ال extra هو و ال tab
+
+451
+00:32:48,210 --> 00:32:50,730
+بتخلص منه في ال west من خلال ان انا اضغط على ال
+
+452
+00:32:50,730 --> 00:32:54,590
+ejector و تنفصل ال tab عن ال pipet و تروح في حال
+
+453
+00:32:54,590 --> 00:33:00,370
+سبيلهمتمام؟ أول حاجة مع .. يعني ملاحظات لاستخدام
+
+454
+00:33:00,370 --> 00:33:04,650
+ال bybit, never point a bybit up ممنوع أنك انت
+
+455
+00:33:04,650 --> 00:33:07,970
+تستخدمي ال bybit تقليبيها بهذا الشكل بحيث انها
+
+456
+00:33:07,970 --> 00:33:13,600
+تكون ال tip صارت فوق و ال bybit تحتطبعا و انا
+
+457
+00:33:13,600 --> 00:33:16,300
+دايما لما بستخدم ال bible بتكون بهذا الاتجاه بحيث
+
+458
+00:33:16,300 --> 00:33:19,620
+أنه التب لا تحت لإنه لو أنا بدي أجلبها ال volume
+
+459
+00:33:19,620 --> 00:33:22,680
+اللي موجود في التب راح يفوت داخل ال bible طبعا هذه
+
+460
+00:33:22,680 --> 00:33:28,420
+عبارة عن أنا بيب طبعا و في كمان نظام ال .. ال ..
+
+461
+00:33:28,420 --> 00:33:33,460
+الذنبركاتفبالتالي ممكن تصدق داخل ال .. يعني يصير
+
+462
+00:33:33,460 --> 00:33:37,300
+صدق او يصير فيه تلف و ممكن في محاليل بترسب بتعمل
+
+463
+00:33:37,300 --> 00:33:41,440
+رواس بتعمل انسداد فهذا بيأثر على دقة ال pipet فهو
+
+464
+00:33:41,440 --> 00:33:45,340
+يفضل ان انا ممنوع اوجهها الى اعلى بحيث ان ال
+
+465
+00:33:45,340 --> 00:33:48,580
+liquid يفوت داخل ال pipet و يعملها destroying
+
+466
+00:33:48,580 --> 00:33:53,060
+تمام؟طيب when with a drawing liquid with with a
+
+467
+00:33:53,060 --> 00:33:56,540
+pipe it always release the plunger slowly طبعا
+
+468
+00:33:56,540 --> 00:34:00,720
+لحظة لاحظته انتوا حتى بنبرت صوتي ركزتي كتير منيح و
+
+469
+00:34:00,720 --> 00:34:04,020
+انا بحكيلكم لما ترفع ايدك عن ال plunger ارفعيه
+
+470
+00:34:04,020 --> 00:34:09,040
+slowly شويه شويه بالتدريج بهدوء مابرفع ايدي فجأة
+
+471
+00:34:09,040 --> 00:34:13,800
+ليش لإن هنا هي بحكيلك انه ال .. ال .. ال ..الشغل
+
+472
+00:34:13,800 --> 00:34:19,280
+رافعت ايدك بشكل تدريجي او بشكل بطيء عن البلانجر
+
+473
+00:34:19,280 --> 00:34:24,080
+هيمنع من انه ال liquid يصير له rushing into انه
+
+474
+00:34:24,080 --> 00:34:28,160
+يندفع داخل ال pipe و بالتالي ممكن يسببلها انسداد
+
+475
+00:34:28,160 --> 00:34:35,100
+او صدق او تلف فبالتاليمهم جدا طبعا انه ارفع ايدي
+
+476
+00:34:35,100 --> 00:34:38,020
+بالتدريج this is especially important with large
+
+477
+00:34:38,020 --> 00:34:41,860
+volumes يعني و هذا الاشي بنتبهله كتير منيح و انا
+
+478
+00:34:41,860 --> 00:34:46,740
+بصحب حجوم كبيرة حجوم كبيرة معناها السائل هيندفع
+
+479
+00:34:46,740 --> 00:34:50,720
+بشكل قوي هو كميته كبيرة فهيفوت داخل ال bipe هيصل ل
+
+480
+00:34:50,720 --> 00:34:55,240
+ال bipe من جوا و يتلفها be sure you use the proper
+
+481
+00:34:55,240 --> 00:35:02,780
+size tip for each bipe حكينا انه لكلبيبت طبعا لكل
+
+482
+00:35:02,780 --> 00:35:08,040
+بيبت في عندى تب خاصة فيها تبعا لحجمها أو تبعا ل ال
+
+483
+00:35:08,040 --> 00:35:12,380
+range تبعها فانا كل بيبت بستخدملها التب الخاصة
+
+484
+00:35:12,380 --> 00:35:15,340
+فيها ما بنفع مثلا مع البيبتات الصغيرة أستخدم تب
+
+485
+00:35:15,340 --> 00:35:19,040
+كبيرة أصلا مش راح تركب عليها وما بنفع مع البيبتات
+
+486
+00:35:19,040 --> 00:35:21,980
+الكبيرة أستخدم تب صغيرة مش راح تكفي لل volume اللي
+
+487
+00:35:21,980 --> 00:35:26,520
+أنا بدني أنقله طبعا فإذا كل بيبت لها تب خاصة فيها
+
+488
+00:35:26,520 --> 00:35:30,740
+بستخدمهاalways use a new tip for each different
+
+489
+00:35:30,740 --> 00:35:35,960
+liquid طبعا كل حجم بدي أنقله بستخدمله a new tip
+
+490
+00:35:35,960 --> 00:35:40,020
+طبعا ال tip جديدة و حكينا انه هاي ال tip أشمالها
+
+491
+00:35:40,020 --> 00:35:44,460
+disposable طبعا فهيمر معانا ان شاء الله مصطلح
+
+492
+00:35:44,460 --> 00:35:48,360
+disposable و أذكركم فيه يعني تستخدم لمرة واحدة فقط
+
+493
+00:35:48,640 --> 00:35:52,580
+طيب use the correct pipet for the volume to be
+
+494
+00:35:52,580 --> 00:35:56,260
+dispended يعني انا بدي استخدم ال pipet اختار ال
+
+495
+00:35:56,260 --> 00:36:02,180
+pipet اللي ال range تبعها تمام مناسب ل .. ال .. ال
+
+496
+00:36:02,180 --> 00:36:04,980
+.. ال pipet ل الحجم اللي بدي أنقله هى مثلا هنا
+
+497
+00:36:04,980 --> 00:36:09,420
+بيحكيلك never use the متين ل الف microliter pipet
+
+498
+00:36:09,420 --> 00:36:12,720
+to dispense متين تمام هى هنا مثلا هى ال range من
+
+499
+00:36:12,720 --> 00:36:18,210
+متين ل الف مابستخدمها عشان انقل حجم متينلأن حكينا
+
+500
+00:36:18,210 --> 00:36:20,870
+إن هي مش هتكون دقيقة على lower limit لكن ممكن تكون
+
+501
+00:36:20,870 --> 00:36:23,630
+دقيقة على upper limit أو بتكون أدقهي على upper
+
+502
+00:36:23,630 --> 00:36:28,750
+limit تمام؟ و لو أنا بدي أخد حجوم أقل من 200 أو
+
+503
+00:36:28,750 --> 00:36:33,790
+أكتر من ألف هذا إيش ممكن يعملي سببلي damage للأداة
+
+504
+00:36:33,790 --> 00:36:39,710
+هذه فبالتالي لازم أكون حذرة و حريصة في استخدام ال
+
+505
+00:36:39,710 --> 00:36:44,850
+bybit without the bybitwithdraw the pipet from the
+
+506
+00:36:44,850 --> 00:36:48,290
+receiving vessels carefully sliding the tip along
+
+507
+00:36:48,290 --> 00:36:51,890
+the wall هلأ مجرد ما انا ارفع ال pipet بعد ما اخد
+
+508
+00:36:51,890 --> 00:36:55,610
+الحجم اللي بديا لازم اعمل حاجة اسمها sliding يعني
+
+509
+00:36:55,610 --> 00:37:00,750
+انا امرر ال tip هادي امسحها على فتحة ال container
+
+510
+00:37:00,750 --> 00:37:05,690
+يعني مثلابهذا الشكل يعني ارفع وانزل ال buy bet على
+
+511
+00:37:05,690 --> 00:37:11,130
+اللي فوهت او فتحت ال container تبع المحلول بحيث
+
+512
+00:37:11,130 --> 00:37:15,910
+انه اي اشي عالق على جدار ال tip من برا اتخلص منه
+
+513
+00:37:15,910 --> 00:37:19,330
+انا بعنيني فقط ال volume اللي انا نقلته في الداخل
+
+514
+00:37:19,330 --> 00:37:23,670
+طبعا فبالتالي عشان ما اخد excess volume remove the
+
+515
+00:37:23,670 --> 00:37:30,770
+tip from the sample liquidتمام؟ و no liquid should
+
+516
+00:37:30,770 --> 00:37:35,130
+remain on the outside of the tip طبعا يعني لما ..
+
+517
+00:37:35,130 --> 00:37:40,130
+مجرد ما انا اطلع من ال .. ال .. يعني اخد الحجم
+
+518
+00:37:40,130 --> 00:37:43,410
+اللي بديها و اطلع من ال container مش لازم يضل
+
+519
+00:37:43,410 --> 00:37:47,910
+بقايا من ال solution على ال tip فيفضل ان انا امسح
+
+520
+00:37:47,910 --> 00:37:52,620
+باستخدام ال wipe تمام؟او ال .. ال جوز او اي قطعة
+
+521
+00:37:52,620 --> 00:37:58,780
+.. قطعة ت .. clinics او قطن او شاش باجفف ال .. ال
+
+522
+00:37:58,780 --> 00:38:02,780
+.. ال bybit بمسحها بمررها عليها بحيث ان انا اجفف
+
+523
+00:38:02,780 --> 00:38:07,300
+اي بقايا عالقة عليها ولكن يف .. بكون حريصة انه
+
+524
+00:38:07,300 --> 00:38:12,600
+هادي ال .. ال .. ال vibe تمامما تلامس فوهة التب
+
+525
+00:38:12,600 --> 00:38:17,540
+ليش لإنها لو لامست فوهة التب هذا absorbent المحارم
+
+526
+00:38:17,540 --> 00:38:21,000
+و ال clinics و الاشي بينطس السائل فممكن تمطس
+
+527
+00:38:21,000 --> 00:38:25,820
+السائل اللي موجود داخل التب و بالتالي بينقص ال
+
+528
+00:38:25,820 --> 00:38:28,560
+volume اللي أخدته و بيكون أنا هيك عم باخد volumes
+
+529
+00:38:28,560 --> 00:38:32,260
+مش accurate و مش precise تمام مهم جدا لها هاي
+
+530
+00:38:32,260 --> 00:38:37,440
+الشغل اذا بعد هى مجرد ما إنه أسحب ال .. ال .. ال
+
+531
+00:38:37,440 --> 00:38:37,860
+.. ال .. ال .. ال .. ال .. ال .. ال .. ال .. ال ..
+
+532
+00:38:37,860 --> 00:38:38,800
+ال .. ال .. ال .. ال .. ال .. ال .. ال .. ال .. ال
+
+533
+00:38:38,800 --> 00:38:39,540
+.. ال .. ال .. ال .. ال .. ال .. ال .. ال .. ال ..
+
+534
+00:38:39,540 --> 00:38:39,680
+ال .. ال .. ال .. ال .. ال .. ال .. ال .. ال .. ال
+
+535
+00:38:39,680 --> 00:38:43,650
+ال .. المش لازم no liquid should remain on the
+
+536
+00:38:43,650 --> 00:38:46,950
+outside مش لازم ال liquid يضل على خارج ال tip
+
+537
+00:38:46,950 --> 00:38:51,470
+فبالتالي بمسح قطارات اللي .. اللي موجودة على ال
+
+538
+00:38:51,470 --> 00:38:56,190
+outside of the tip بستخدام tissue, paper, جوز,
+
+539
+00:38:56,730 --> 00:39:01,130
+wipes أي إش .. أي نوع محارب أو clinics بحيث انه ما
+
+540
+00:39:01,130 --> 00:39:04,790
+ألامس never touch the tip orifice مافتحق ماألامسش
+
+541
+00:39:04,910 --> 00:39:08,290
+ال tip orifice يعني فتحة ال .. ال tip ال opening
+
+542
+00:39:08,290 --> 00:39:12,410
+تبع ال tip طبعا ماقلامسه بحيث انه ماينطست السائل و
+
+543
+00:39:12,410 --> 00:39:16,770
+بهك انا اكون عم باخد volume اقل من اللازم و اكون
+
+544
+00:39:16,770 --> 00:39:19,870
+مش .. مش accurate و مش precise في عملية ال volume
+
+545
+00:39:19,870 --> 00:39:25,090
+transfer طبعا طيب pre-rinsing ايش يعني كلمة pre
+
+546
+00:39:25,090 --> 00:39:31,610
+-rinsing يفضل انه انا .. انا لما بدي انقل حجوم ان
+
+547
+00:39:31,610 --> 00:39:39,390
+انا ابلليعني مثلا هنا يفضل إن أنا أبلل ال .. ال ..
+
+548
+00:39:39,390 --> 00:39:44,290
+التب من جوا في .. في .. في السائل اللي بدي أنقله،
+
+549
+00:39:44,290 --> 00:39:47,510
+هاي معناه pre runs، طبعا؟ يعني أول حاجة بصحب أول
+
+550
+00:39:47,510 --> 00:39:52,330
+volume و بتخلص منه بكبه، هيك أنا بضمن إنه جزء من
+
+551
+00:39:52,330 --> 00:39:56,570
+السائل لمس ال .. ال .. التب من الداخل فبالتالي
+
+552
+00:39:56,570 --> 00:40:02,570
+صارت التب إشمالها مبللة، طيب فلمّا أجي أنقل volume
+
+553
+00:40:03,230 --> 00:40:06,390
+و أاخد ال volume و أنقله هيك بكون أنا عم باخد
+
+554
+00:40:06,390 --> 00:40:10,490
+volume بشكل أدق ليش؟ لأنه لو .. لو أنا ماعملت ال
+
+555
+00:40:10,490 --> 00:40:14,490
+pre-rinsing هذه طبعا لو ماعملت pre-rinsing أخدت
+
+556
+00:40:14,490 --> 00:40:18,350
+volume و روحت فضيته في جزء من السائل اللي أنا
+
+557
+00:40:18,350 --> 00:40:24,370
+أخدته بيضل ملتصق بجدران التب مبلل لإلها وهذا جزء
+
+558
+00:40:24,370 --> 00:40:29,190
+يعني في نسبة فقد لل volume في نسبة errorطبعا لكن
+
+559
+00:40:29,190 --> 00:40:34,490
+لما انا استخدم tip already مبللة يعني tip انا سحبت
+
+560
+00:40:34,490 --> 00:40:36,970
+اول volume فيها و كبيته و بعدين سحبت ال volume
+
+561
+00:40:36,970 --> 00:40:40,290
+اللى بدي استخدمه ال volume الفعلي معناه انا بللت
+
+562
+00:40:40,290 --> 00:40:45,850
+ال tip من الداخل فبالتالي فبطل في عندي نسبة فقد من
+
+563
+00:40:45,850 --> 00:40:49,890
+الحجم و بطل في عندي نسبة ال error هذه معناه كلمة
+
+564
+00:40:49,890 --> 00:40:53,670
+pre-rinsing minimize اشمالها ال variances
+
+565
+00:40:53,670 --> 00:40:59,560
+الاختلافات في ال .. ال ..الحجوم طبعا when by
+
+566
+00:40:59,560 --> 00:41:03,080
+beating some solutions زي ال serum اللي هي العينات
+
+567
+00:41:03,080 --> 00:41:08,260
+يعني الشخ .. المرضى ال serum البلازما ال protein
+
+568
+00:41:08,260 --> 00:41:11,300
+containing solution المحليل اللي بتحتوي على
+
+569
+00:41:11,300 --> 00:41:14,420
+البروتينات ال organic solvents المضيبات العضوية
+
+570
+00:41:14,420 --> 00:41:19,700
+هذه طبعا في جزء منها بيكون حكينا انه هيصير في فيلم
+
+571
+00:41:19,700 --> 00:41:24,500
+هيفي جزء منها هيبطن هيلامس ال inside of the tipو
+
+572
+00:41:24,500 --> 00:41:29,200
+هذا حكينا اشماله error فبالتالي لما انا اعمل عملية
+
+573
+00:41:29,200 --> 00:41:33,540
+pre-rinsing فبالتالي انا بقلل ال error هذه من خلال
+
+574
+00:41:33,540 --> 00:41:39,120
+ان انا خلاص استخدمت already جزء بيبة مبللة جزء ..
+
+575
+00:41:39,120 --> 00:41:43,040
+بيبة عملنا لها pre-rinsing فبالتالي مش هيكون في
+
+576
+00:41:43,040 --> 00:41:47,400
+عندي فقد في ال volume و مش هيكون في عندي نسبة
+
+577
+00:41:47,400 --> 00:41:51,780
+errorطيب when by beating concentrated acid or
+
+578
+00:41:51,780 --> 00:41:56,100
+highly corrosive solution such as halite salts
+
+579
+00:41:56,100 --> 00:42:01,540
+it's recommended to that by bit be disassembled
+
+580
+00:42:01,540 --> 00:42:07,680
+after used to inspect the shaft و المكونات تبعتها،
+
+581
+00:42:07,680 --> 00:42:11,800
+هلأ بيحكيلك أنه لما بنستخدم بعض المحاليل اللي هي
+
+582
+00:42:11,800 --> 00:42:18,100
+corrosive تمامحارقة او يعني مؤدية وبيتطير منها
+
+583
+00:42:18,100 --> 00:42:24,160
+ابخرة او يعني فيه بيتصعد منها قترانات او ابخرة او
+
+584
+00:42:24,160 --> 00:42:28,960
+رذاذ زي ال .. ال .. الهاليد سالتز هاي فيفضل ان انا
+
+585
+00:42:28,960 --> 00:42:32,620
+افك البعيبة و انظفها من الداخل و ارجع اركبها مرتان
+
+586
+00:42:32,620 --> 00:42:38,940
+علشان اتأكد انها هي يعني ما رح تتعطل ما رح تخرب ال
+
+587
+00:42:38,940 --> 00:42:44,050
+air bubbleطبعا يعني ان في عندي تقطع في السائل اللي
+
+588
+00:42:44,050 --> 00:42:47,170
+انا عم باخده في هنا فيه قطارات في فيه .. فيه هوا
+
+589
+00:42:47,170 --> 00:42:52,670
+تمام؟طب هذا ال .. ال .. الهواء إيش سببه؟ سببه أول
+
+590
+00:42:52,670 --> 00:42:55,970
+حاجة إما إن أنا رفعت إيدي عن ال plunger بشكل سريع
+
+591
+00:42:55,970 --> 00:43:00,010
+أو كانت ال tip مش مغمورة كويس في ال solution سحبت
+
+592
+00:43:00,010 --> 00:43:03,550
+من عن السطح فبالتالي بما إن سحبت عن السطح معناها
+
+593
+00:43:03,550 --> 00:43:10,810
+صار في عرض لإنه يدخل air bubble تمام ممكن يكون إنه
+
+594
+00:43:11,460 --> 00:43:19,940
+سببها ال .. ال .. يعني السائل نفسه يكون يعني فومي
+
+595
+00:43:19,940 --> 00:43:26,480
+بي .. بيكون رغوة فبالتالي انا ما اتبعت التعليمات ل
+
+596
+00:43:26,480 --> 00:43:31,360
+.. ل .. ل .. ال by betting من فومي reagent
+
+597
+00:43:31,360 --> 00:43:36,240
+فبالتالي ممكن تظهر عندى air bubble طبعا ال .. ال
+
+598
+00:43:36,240 --> 00:43:40,190
+.. ال air bubble إذا ظهرت طبعاطبعا أول حاجة إذا
+
+599
+00:43:40,190 --> 00:43:43,910
+ظهرت في .. في ال by bit بفضل solution و برجع باخد
+
+600
+00:43:43,910 --> 00:43:47,490
+مرة تانية، ضالت موجودة معناها بيده أتخلص من التب و
+
+601
+00:43:47,490 --> 00:43:51,090
+أركب تب جديدة و أاخد volume مع ال .. التزام
+
+602
+00:43:51,090 --> 00:43:56,410
+بالتعليمات حتى أتجنب من ظهور air bubble، ليش؟ لإن
+
+603
+00:43:56,410 --> 00:43:59,150
+ال air bubble طبعا هذه محتلة جزء من الحجم اللي
+
+604
+00:43:59,150 --> 00:44:03,580
+موجود يعني هذا المفروض كله يكون سائلطبعا فوجود air
+
+605
+00:44:03,580 --> 00:44:06,860
+bubble وجود تقطع في السائل يعني فيه هوى فبالتالي
+
+606
+00:44:06,860 --> 00:44:09,900
+الهوى محتل حجم اذا انا مش ماخدة الحجم المناسب
+
+607
+00:44:09,900 --> 00:44:14,360
+ماخدة اقل منه اذا انا مش accurate ومش precise طبعا
+
+608
+00:44:14,360 --> 00:44:19,420
+هذا بالنسبة ل ال bybit طيب خلينا بس قبل ما نشوف ال
+
+609
+00:44:19,420 --> 00:44:23,580
+calibration ل ال bybit نتابع مع بعض نشوف هذا
+
+610
+00:44:23,580 --> 00:44:30,040
+الفيديو بيعلمناكيف انا بدي استخدم ال .. ال by bet
+
+611
+00:44:30,040 --> 00:44:34,000
+و هنرجع نحكي نفس التعليمات او نفس المعلومات اللي
+
+612
+00:44:34,000 --> 00:44:37,560
+انا ذكرتهم باستخدام ال by bet هاي زي ما انتوا
+
+613
+00:44:37,560 --> 00:44:42,340
+شايفين هنا خليني بس ارجع شوية هنا تمام هاي ال by
+
+614
+00:44:42,340 --> 00:44:48,640
+bet نفس المكونات زي ما انتوا شايفين هنا نفس
+
+615
+00:44:48,640 --> 00:44:52,400
+المكونات لحظة خليني ارجع
+
+616
+00:44:55,930 --> 00:44:59,770
+خلص من هاي، هاي ال pipe هاي البلانجر تمام و الرد
+
+617
+00:44:59,770 --> 00:45:03,090
+هذا اللي بيلتف يمين و يسار تمام عكس حقارب الساعة و
+
+618
+00:45:03,090 --> 00:45:06,470
+باتجاه حقارب الساعة باتجاهي او بعيد عني حسب ما انا
+
+619
+00:45:06,470 --> 00:45:10,930
+هعلمكم ان شاء الله و هاي ال .. ال .. ال screen
+
+620
+00:45:10,930 --> 00:45:16,350
+تمام هذا ال .. ال .. ال ejector طبع التب على الجهة
+
+621
+00:45:16,350 --> 00:45:21,470
+التانية في هنا ال adapter هذا ال handle طبع ال ..
+
+622
+00:45:23,100 --> 00:45:27,640
+البيبت وهنا الموجود فيه ال adapter تبع التب تمام
+
+623
+00:45:27,640 --> 00:45:33,970
+ومهي المكونات كلها موجودة طيب خلينا نشوفكيف بيتم
+
+624
+00:45:33,970 --> 00:45:38,030
+تحويل حجم يعني من خلال .. يعني إما ب .. في بيبتات
+
+625
+00:45:38,030 --> 00:45:42,250
+بلف ال .. ال .. بلانجر نفسه بكون بحاركه، في بيبتات
+
+626
+00:45:42,250 --> 00:45:48,030
+بيكون لأ، في بيكون زي منظم موجود لأسفل منها و من
+
+627
+00:45:48,030 --> 00:45:51,970
+خلاله أنا بتحكم في الحجم، إذا بلفه علشان أتحكم
+
+628
+00:45:51,970 --> 00:45:56,840
+بالحجم اللي موجود هنا، شايفين؟ ها يعني تمام؟زي ما
+
+629
+00:45:56,840 --> 00:46:01,760
+انتوا شايفين بحرك بحيث ان انا اختار الحجم اللى بدى
+
+630
+00:46:01,760 --> 00:46:05,240
+طبعا ال by bit هى مكتوب هنا بتاخد من مية لألف هينا
+
+631
+00:46:05,240 --> 00:46:10,240
+ضبطناها على الف و بدى بعد هيك اروح اركبلها التب
+
+632
+00:46:10,240 --> 00:46:14,440
+الخاصة فيها طبعا لازم تكون تب مناسبة لإلها شايفين
+
+633
+00:46:14,440 --> 00:46:17,940
+التبات طبعا disposable يا بنات ال by bit التب اللى
+
+634
+00:46:17,940 --> 00:46:21,720
+بستخدمها برجعهاش مكانها بتخلص منها على طول هى التب
+
+635
+00:46:21,720 --> 00:46:29,190
+تمام هنا بيكون في هذه التب بيكون عندىsorry في هذه
+
+636
+00:46:29,190 --> 00:46:31,890
+ال tip بيكون في عندى الحجم اللى انا بدى انجله هاي
+
+637
+00:46:31,890 --> 00:46:35,830
+البلانجة طبعا انا لما اجى اكبس عليه بيفي عندى
+
+638
+00:46:35,830 --> 00:46:38,850
+first stop هاي first stop شايفين هادى اول
+
+639
+00:46:38,850 --> 00:46:42,190
+resistant بتقابلني هاي في عندى اذا هادى ال first
+
+640
+00:46:42,190 --> 00:46:47,900
+stop شايفين هنامجرد هي ضغطت فاتت عمودي في داخل
+
+641
+00:46:47,900 --> 00:46:52,680
+السائل طبعا خلينا ال tip يعني تكون مغمورة داخل
+
+642
+00:46:52,680 --> 00:46:57,400
+السائل و بنبدأ نرفع شوي شوي بالتدريج ايدنا عن
+
+643
+00:46:57,400 --> 00:47:01,540
+البلانجر ايش اللي هيصير بالظبط هيصير ان دخول
+
+644
+00:47:01,540 --> 00:47:05,220
+السائل داخل ال tip و هيك انا بكون أخدت الحجم اللي
+
+645
+00:47:05,220 --> 00:47:09,020
+موجود على ال screen طبعا لحظة انها ضغطت ضغطة واحدة
+
+646
+00:47:09,020 --> 00:47:13,660
+يعني هي ايش بتعملبتكون .. بتكون عم تعمل forward by
+
+647
+00:47:13,660 --> 00:47:17,900
+betting طبعا فهذا ال by bet او هذا ال volume بروح
+
+648
+00:47:17,900 --> 00:47:21,100
+باخده في container و بفضيه من خلال ضغطة أولى او
+
+649
+00:47:21,100 --> 00:47:24,140
+ضغطة تانية طبعا هاي شايفين الضغطة التانية هذه هي
+
+650
+00:47:24,140 --> 00:47:27,420
+الضغطة التانية طبعا هاي .. طبعا اذا انا .. انا هاي
+
+651
+00:47:27,420 --> 00:47:32,120
+هنا الضغطة بانقل ب .. باخد ال volume و بنقله لهذا
+
+652
+00:47:32,120 --> 00:47:41,050
+بفوت عمودي و لازم اتبع كل التعليمات طبعالو رجع
+
+653
+00:47:41,050 --> 00:47:48,310
+نركز عليها مرة تانية نشوف
+
+654
+00:47:48,310 --> 00:47:53,490
+هنا هى
+
+655
+00:47:53,490 --> 00:47:57,830
+هى هنا شايفين هادى الضغط
+
+656
+00:48:02,970 --> 00:48:07,490
+هي ضغطة وهي الضغطة التانية شايفين؟ هى معانا ضغطة
+
+657
+00:48:07,490 --> 00:48:13,530
+وضغطتين طبعا؟ طيب و هيك بيتم نقل حجوم بالشكل هذا
+
+658
+00:48:13,530 --> 00:48:17,800
+من الى طبعا؟ من الىال container الأصلي ل ال
+
+659
+00:48:17,800 --> 00:48:20,380
+container اللى بدى أشتغل فيه بنقل حجم اللى بديه
+
+660
+00:48:20,380 --> 00:48:25,340
+علشان أنا بكون بدى أجري تجربة معينة تمام لما باجى
+
+661
+00:48:25,340 --> 00:48:29,620
+بفضى لازم أنا أضغط على البلانجر بحيث أنه يفضى طبعا
+
+662
+00:48:29,620 --> 00:48:33,200
+البلانجر بضغط عليه بسحب بضغط عليه مرتان يدفضى و
+
+663
+00:48:33,200 --> 00:48:38,680
+هكذا يفضل يا بنات لما أنا أجى أسحب طبعا اللى أنتوا
+
+664
+00:48:38,680 --> 00:48:47,870
+شايفين هنا هي ال ..هنا شايفين كيف انا بفوت ضغطة
+
+665
+00:48:47,870 --> 00:48:51,790
+تمام لو
+
+666
+00:48:51,790 --> 00:48:52,390
+نرجع
+
+667
+00:48:55,290 --> 00:48:59,850
+تمام هى هنا فضة ال volume هى هنا ضغطة هى ضغطتين
+
+668
+00:48:59,850 --> 00:49:03,690
+تمام تعلمنا الضغطة و الضغطتين و هى هنا شايفين كده
+
+669
+00:49:03,690 --> 00:49:08,430
+أنا بفوت ضغطة و بعدين ب .. بسحب ال .. ب .. بترك
+
+670
+00:49:08,430 --> 00:49:12,630
+إيدي شوية شوية عن البلانجر مابنفع أنا أفوت مش ضغطة
+
+671
+00:49:12,630 --> 00:49:16,250
+و أضغط جوا ال solution ليش؟ لإنه ساعتها أنا بكون
+
+672
+00:49:16,250 --> 00:49:18,890
+بدخل هوا جوا ال solution إذا هو من النوع الفومي
+
+673
+00:49:18,890 --> 00:49:21,890
+اللى بيكون رغوة هيصير فيه رغوات و يصير في عندى air
+
+674
+00:49:21,890 --> 00:49:26,950
+bubble و مشاكل في نقل الحجومطبعا طيب هلأ انا بعد
+
+675
+00:49:26,950 --> 00:49:29,730
+ما انا فضيت ال volume ضغطت على البلانجر و فضيت ال
+
+676
+00:49:29,730 --> 00:49:32,350
+volume اش بده اعمل بده اضغط على ال ejector عشان
+
+677
+00:49:32,350 --> 00:49:36,370
+اتخلص من التب شايفين مجرد ما اضغط عليها ال .. ال
+
+678
+00:49:36,370 --> 00:49:40,890
+.. التب بتفصل عن ال bibert و بت .. بتم التخلص منها
+
+679
+00:49:40,890 --> 00:49:46,460
+طبعاهذا بالنسباله ال .. ال .. ال bybite اذا اول
+
+680
+00:49:46,460 --> 00:49:52,560
+حاجة بنحدد الحجم ب .. أركب ال tip بعمل rinsing pre
+
+681
+00:49:52,560 --> 00:49:58,200
+-rinsing ببللها تمام و بعدين باخد الحجم اللي بدياه
+
+682
+00:49:58,200 --> 00:50:01,960
+سواء كان باخده بطريقة ال .. ال .. ال forward
+
+683
+00:50:01,960 --> 00:50:06,000
+bybiting او ال reverse bybiting حسب اللي انا الحجم
+
+684
+00:50:06,000 --> 00:50:09,930
+اللي ال .. ال .. او السائل اللي انا بتعامل معاهو
+
+685
+00:50:09,930 --> 00:50:15,030
+بانقله من و إلى بعد هيك بتخلص من التب بهذا الشكل
+
+686
+00:50:15,030 --> 00:50:21,550
+تمام؟ ان شاء الله هيكون شغلنا على ال .. ال bybit و
+
+687
+00:50:21,550 --> 00:50:24,630
+طبعا معظم .. كل الفحوصات بتنعمل باستخدام ال bybit
+
+688
+00:50:24,630 --> 00:50:28,630
+انا بدي اضيف حجم من ال reagent و حجم من العينة حجم
+
+689
+00:50:28,630 --> 00:50:31,510
+من ال standard و حجم من ال reagent كل شغلي
+
+690
+00:50:31,510 --> 00:50:33,710
+باستخدام ال bybit اذا هذه صديقتنا هذه اللي
+
+691
+00:50:33,710 --> 00:50:36,390
+هترافقنا من شاء الله من سنة تانية لسنة رابعة لجهة
+
+692
+00:50:36,390 --> 00:50:39,950
+الأداء فبالتالي لازم نكون دقيقين في استخدامهاو
+
+693
+00:50:39,950 --> 00:50:42,690
+عشان .. عشان نكون دقيقين باستخدامها و تكون ال vibe
+
+694
+00:50:42,690 --> 00:50:46,370
+تهدي احنا بنعمللها صيانة و بنضمن دقتها باستمرار
+
+695
+00:50:46,370 --> 00:50:49,530
+لازم انه انا اعمللها calibration اش يعني
+
+696
+00:50:49,530 --> 00:50:52,890
+calibration يعني اعادة ضبط وتهيئة علشان اتأكد من
+
+697
+00:50:52,890 --> 00:50:57,070
+شغلها ان هي عم تاخدلي شغل دقيق طبعا كيف انا بدي
+
+698
+00:50:57,070 --> 00:51:01,660
+اخد ال .. ال .. اكيد اعمللها calibration؟بدي
+
+699
+00:51:01,660 --> 00:51:06,200
+استخدمها يعني او اظبط شغلها او اتأكد من انها عم
+
+700
+00:51:06,200 --> 00:51:10,820
+تاخد volumes بشكل دقيق باستخدام الحجوم الماء ال
+
+701
+00:51:10,820 --> 00:51:14,960
+distilled water تمام؟ هنا بيحكيلي انه تحت درجة
+
+702
+00:51:14,960 --> 00:51:21,200
+الحرارة و الضغط الثابت الكثافة الماء أيضا ثابتة
+
+703
+00:51:21,200 --> 00:51:26,860
+فالحجم من الماء تمام ممكن انا يعني one ml من الميه
+
+704
+00:51:26,860 --> 00:51:31,820
+بيعادل one gramمن الميه تمام ال .. ال one ml ال
+
+705
+00:51:31,820 --> 00:51:36,360
+volume one ml لو أنا جيت وزنته تحت درجة حرارة وضغط
+
+706
+00:51:36,360 --> 00:51:40,400
+ثابت وكثافة ثابتة للماء فهي .. ال .. ال one ml
+
+707
+00:51:40,400 --> 00:51:46,710
+هيعطيني one gram من ال water تمام؟ طيب فبالتاليال
+
+708
+00:51:46,710 --> 00:51:51,330
+.. ال calibration لل بايبت لازم انا اكون بس يعني
+
+709
+00:51:51,330 --> 00:51:54,390
+بدي اعرف ال volume اللي عم تاخده حجم الماء اللي
+
+710
+00:51:54,390 --> 00:51:59,890
+بتاخده و هل هو accurate او precise و طبعا هذا
+
+711
+00:51:59,890 --> 00:52:06,910
+بيتأثر بدرجة حرارة الغرفة و ايضا الضغط الجوي و
+
+712
+00:52:06,910 --> 00:52:10,190
+الرطوبة نسبة الرطوبة اللي موجودة في الوسط او في
+
+713
+00:52:10,190 --> 00:52:14,320
+البيئة المحيطة لإجراء التجربةعشان هيك انا بستخدم
+
+714
+00:52:14,320 --> 00:52:17,980
+factor او ثابت اسمه z factor في الحسابات طبعتي
+
+715
+00:52:17,980 --> 00:52:23,340
+هنحكي عنه كمان شوية هذا ال z factor فالفكرة طبعتي
+
+716
+00:52:23,340 --> 00:52:27,660
+انه انا بدي اخد one ml من ال distilled water يعني
+
+717
+00:52:27,660 --> 00:52:30,980
+بدي اجيب ال by bit بدي اعمللها setting بدي ا ..
+
+718
+00:52:30,980 --> 00:52:35,700
+اضب .. اضبطها على one ml اركب tip اخد اعمل ال pre
+
+719
+00:52:35,700 --> 00:52:39,920
+-rinsing ابلل ال tip من .. و اتخلص من ال solution
+
+720
+00:52:39,920 --> 00:52:44,350
+اللي أخدته في المرة الأولىبعد هيك إيش بدي أعمل؟
+
+721
+00:52:44,350 --> 00:52:50,110
+بدي أخد one ml من distilled water و أحطه في cup ال
+
+722
+00:52:50,110 --> 00:52:53,990
+cup هذا بدي أكون مسفر عليه في الميزان بدي أسفر
+
+723
+00:52:53,990 --> 00:52:57,590
+عليه و أحط ال one ml و أشوف الوزنة تبعتي الوزنة
+
+724
+00:52:57,590 --> 00:53:00,090
+تبعتي هتكون إذا هو مسفر على ال cup فالوزنة اللي
+
+725
+00:53:00,090 --> 00:53:05,050
+هتطلع معايا هي وزنت ال one ml المفروض تكون مقاربة
+
+726
+00:53:05,050 --> 00:53:08,470
+ل one gram تمام؟ المفروض انها تكون مقاربة ل one
+
+727
+00:53:08,470 --> 00:53:13,310
+gram او .. او يعني exactly one gramطبعا فعشان ان
+
+728
+00:53:13,310 --> 00:53:16,130
+احنا عندنا درجة الحرارة و الدغط الجوي و ال
+
+729
+00:53:16,130 --> 00:53:21,070
+humidity متغيرة فانا بروح بدرب الوزن هذه تبعتي بال
+
+730
+00:53:21,070 --> 00:53:25,390
+Z factor و في جدول في اخر ال .. ال .. ال slide
+
+731
+00:53:25,390 --> 00:53:29,590
+بعلمني كيف انا اختار ال Z factor المناسب اللي بدي
+
+732
+00:53:29,590 --> 00:53:36,880
+ادرب في القراءات تبعتي طبعا فإذا ال ..الحجم هي هنا
+
+733
+00:53:36,880 --> 00:53:41,300
+مثلا إيش بلزمني sorry إيش بلزمني لأ التجربة تبعتي؟
+
+734
+00:53:41,300 --> 00:53:46,100
+بلزمني by bit و tip بلزمني beaker أو cup لحتى أحطه
+
+735
+00:53:46,100 --> 00:53:49,220
+على الميزان و أصفر عليه تمام؟ بلزمني distilled
+
+736
+00:53:49,220 --> 00:53:54,260
+water بلزمني أقيس درجة الحرارة و ميزان و أقيس ال
+
+737
+00:53:54,260 --> 00:54:00,850
+pressure الدغط عشان أطلع z factorأول إشي بدي أقيس
+
+738
+00:54:00,850 --> 00:54:07,510
+درجة حرارة الماء و أسجلها بعد هيك باستخدام beaker
+
+739
+00:54:07,510 --> 00:54:10,950
+طبعا انه انا بدي أحطه على ال .. طبعا مش راح أقرأ
+
+740
+00:54:10,950 --> 00:54:18,900
+كل الخطوات هايراح اختصرها باستخدام ال beaker او ال
+
+741
+00:54:18,900 --> 00:54:23,080
+cup راح احطه على ال analytical balance و راح هصفر
+
+742
+00:54:23,080 --> 00:54:27,080
+عليه طبعا دي خلي ا .. ا .. اخلي الميزان يعتبر ان
+
+743
+00:54:27,080 --> 00:54:32,040
+وزن ال cup هذا zero طبعابعد هيك بدي أعمل pre ..
+
+744
+00:54:32,040 --> 00:54:35,520
+بدي أركب تب للبيبط و بعد ما أكون أنا حددت الحجم
+
+745
+00:54:35,520 --> 00:54:40,840
+المناسب لإلها اللي هو one ml يعني الف microliter و
+
+746
+00:54:40,840 --> 00:54:44,940
+بعد هيك بدي أعمل pre-rinsing عرفنا إيش يعني pre
+
+747
+00:54:44,940 --> 00:54:49,040
+-rinsing يعني أنا بدي أسحب أسبيرات and spins يعني
+
+748
+00:54:49,040 --> 00:54:53,640
+بدي أسحب و أتخلص من الحجم على أساس ممكن أعيد هذه
+
+749
+00:54:53,640 --> 00:54:56,860
+الخطوة تلت مرات على أساس أتأكد أنه ال .. ال ..
+
+750
+00:54:56,860 --> 00:54:59,580
+التب صارت مبللة من الداخل يعني مش هيكون في عندى لا
+
+751
+00:54:59,580 --> 00:55:05,320
+.. لا فقطولا error في ال volume تمامو بعد هيك إيش
+
+752
+00:55:05,320 --> 00:55:09,880
+بدي أعمل؟ بدي أضغط على ال .. ال .. ال .. ال .. ال
+
+753
+00:55:09,880 --> 00:55:11,120
+.. ال .. ال .. ال .. ال .. ال .. ال .. ال .. ال ..
+
+754
+00:55:11,120 --> 00:55:11,380
+ال .. ال .. ال .. ال .. ال .. ال .. ال .. ال .. ال
+
+755
+00:55:11,380 --> 00:55:11,580
+.. ال .. ال .. ال .. ال .. ال .. ال .. ال .. ال ..
+
+756
+00:55:11,580 --> 00:55:13,040
+ال .. ال .. ال .. ال .. ال .. ال .. ال .. ال .. ال
+
+757
+00:55:13,040 --> 00:55:13,160
+.. ال .. ال .. ال .. ال .. ال .. ال .. ال .. ال ..
+
+758
+00:55:13,160 --> 00:55:13,660
+ال .. ال .. ال .. ال .. ال .. ال .. ال .. ال .. ال
+
+759
+00:55:13,660 --> 00:55:14,400
+ال .. ال .. ال .. ال .. ال .. ال .. ال .. ال .. ال
+
+760
+00:55:14,400 --> 00:55:21,420
+.. ال .. ال ..
+
+761
+00:55:21,420 --> 00:55:21,440
+ال .. ال .. ال .. ال .. ال .. ال .. ال .. ال .. ال
+
+762
+00:55:21,440 --> 00:55:25,140
+.. ال ..
+
+763
+00:55:34,190 --> 00:55:40,910
+عشان السائل يفوت داخل التب أيضا بشكل متأني يعني
+
+764
+00:55:40,910 --> 00:55:44,210
+انه مايصير فيه اندفاع لا solution و هي air bubble
+
+765
+00:55:44,210 --> 00:55:48,260
+و المشاكل اللي ممكن تحدث في البيت انجلتمام؟ هيك
+
+766
+00:55:48,260 --> 00:55:51,980
+أنا أخدت الحجم اللي بدياه بروح بفضيه في ال beaker
+
+767
+00:55:51,980 --> 00:55:55,340
+أو ال cup اللي موجود على الميزان و باخد القراءة
+
+768
+00:55:55,340 --> 00:55:59,760
+تمام؟ بعد هيك ممكن أنا أرجع أصفر على ال cup و ال
+
+769
+00:55:59,760 --> 00:56:03,420
+.. ال volume اللي موجود فيه أو أن أنا أستخدم cup
+
+770
+00:56:03,420 --> 00:56:08,100
+جديد إيه للخيار؟بسفر و بعيد الخطوة مرة تانية باخد
+
+771
+00:56:08,100 --> 00:56:13,560
+one amal بالبيبت و بفضيه و باخد كمان وزنة و كمان
+
+772
+00:56:13,560 --> 00:56:17,340
+مرة تالتة باخد one amal و بفضيه في الميزان و باخد
+
+773
+00:56:17,340 --> 00:56:22,320
+وزنة تالتة فإذا صار في عندي تلت وزنات لتلت حجوم
+
+774
+00:56:22,320 --> 00:56:25,400
+تمام فإيش بدي أعمل في التلت وزنات هدول؟ بدي أجيب
+
+775
+00:56:25,400 --> 00:56:28,740
+ال average تبعهم يعني بدي أجيب مجموعهم على تلتة
+
+776
+00:56:29,100 --> 00:56:34,400
+تمام؟ ال .. هذه ال ..ش اسمه؟ ال .. يعني تكملت
+
+777
+00:56:34,400 --> 00:56:37,220
+التجربة اللي انا حكيتها يعني انه .. انه عيدي
+
+778
+00:56:37,220 --> 00:56:41,380
+الخطوات تلت مرات و جيبي القراءة هي هنا مثلا
+
+779
+00:56:41,380 --> 00:56:44,340
+بيحكيلي ممكن تعديها اربعتاشر مرات حسب يعني ممكن
+
+780
+00:56:44,340 --> 00:56:48,200
+تعديها تلت مرات اربع مرات خمس مرات المهم بالنهاية
+
+781
+00:56:48,200 --> 00:56:51,640
+الوزنات اللي تطلع معاكي بدك تاخديلها average ايش
+
+782
+00:56:51,640 --> 00:56:56,440
+يعني average؟ يعني مجموعها على عددهاو اطلع قراءة
+
+783
+00:56:56,440 --> 00:56:59,680
+واحدة القراءة واحدة هي هذه اللي بدك تروحي اتعاوضي
+
+784
+00:56:59,680 --> 00:57:02,940
+فيها بالقانون ال weight او ال average of weight
+
+785
+00:57:02,940 --> 00:57:06,180
+اللي طلع معاكي و بدك تضربيه في ال z factor اللي هو
+
+786
+00:57:06,180 --> 00:57:10,980
+معامل اللي هو الحرارة و الضغط تمام اللي موجود عندك
+
+787
+00:57:10,980 --> 00:57:14,980
+و بالنهاية بحسب ال volumeانا خدت ال bybit كانت
+
+788
+00:57:14,980 --> 00:57:18,940
+بتحكيلي ان انا باخد one ml على الشاشة تبع ال bybit
+
+789
+00:57:18,940 --> 00:57:22,840
+مكتوب one ml فالمفروض ان هذا الكلام ال wet في ال
+
+790
+00:57:22,840 --> 00:57:27,020
+zip factor يكون one ml ايضا يعني او مقارب ل one ml
+
+791
+00:57:27,020 --> 00:57:34,050
+فممكن اناأطبق قوانين التحليلية يعني هي ان انا بجيب
+
+792
+00:57:34,050 --> 00:57:39,050
+ال average لقراءات الوزنة اللي هي مجموع القراءات
+
+793
+00:57:39,050 --> 00:57:43,170
+على عددها تمام ممكن اطبق ايضا احسب ال standard
+
+794
+00:57:43,170 --> 00:57:47,870
+deviation اجيب ال accuracy يعني القيمة الوزنة اللي
+
+795
+00:57:47,870 --> 00:57:50,610
+طلعت معايا و اللي المفروض تطلع مثلا طلعت معايا
+
+796
+00:57:50,610 --> 00:57:54,690
+point 999 الوزنة و المفروض انها تطلع one milligram
+
+797
+00:57:54,690 --> 00:57:58,280
+بحسبهابس شوف .. شوف نسبة ال accuracy تبعت القيمة
+
+798
+00:57:58,280 --> 00:58:02,100
+تبعتي و ال coefficient of variation اللي هي ..
+
+799
+00:58:02,100 --> 00:58:04,560
+اللي هي عبارة عن ال standard deviation على ال mean
+
+800
+00:58:04,560 --> 00:58:11,920
+ضارب 100% لازم ال accuracy هذه تطلع من 99 ل 101% و
+
+801
+00:58:11,920 --> 00:58:16,470
+لازمال coefficient of variation يكون اقل من واحد
+
+802
+00:58:16,470 --> 00:58:19,690
+في المية هذا يكون اقل من واحد في المية عشان اقول
+
+803
+00:58:19,690 --> 00:58:22,290
+ان ال by bit هذه accurate و precise و بتعطيني
+
+804
+00:58:22,290 --> 00:58:25,570
+volumes دقيقة و الحجم اللي انا باخده هو نفسه اللي
+
+805
+00:58:25,570 --> 00:58:28,990
+موجود على الشاشة و شغلها مية لمية اذا انا لما بدي
+
+806
+00:58:28,990 --> 00:58:31,930
+اجري افحصات و بدي انقل volume باستخدام هذه ال by
+
+807
+00:58:31,930 --> 00:58:36,740
+bit اذا انا عم بشتغل بشكل صحيح و بشكل دقيقطبعا هذا
+
+808
+00:58:36,740 --> 00:58:40,740
+الجدول اللى انا من خلاله بختار مثلا ال .. ال z
+
+809
+00:58:40,740 --> 00:58:45,180
+value او اللى هو معامل ال .. الحرارة و الضغط طبعا
+
+810
+00:58:45,180 --> 00:58:47,980
+ال z value مثلا انا بشوف ايش الضغط اللى موجود عندى
+
+811
+00:58:47,980 --> 00:58:50,040
+حسب المنطقة اللى انا .. اللى انا فيها لان افرض
+
+812
+00:58:50,040 --> 00:58:54,620
+مثلا ان انا موجودة في منطقة ضغطها الف و ست .. سبعة
+
+813
+00:58:54,620 --> 00:58:59,420
+و ستين ملي بار طبعاو درجة الحرارة عندي خمسة و
+
+814
+00:58:59,420 --> 00:59:04,240
+عشرين فبروح هنا هي خمسة و عشرين بمشي لعند مين لعند
+
+815
+00:59:04,240 --> 00:59:08,100
+ال .. ال .. ال .. المنطقة اللي هي ال .. اللي فيها
+
+816
+00:59:08,100 --> 00:59:11,600
+الضغط تبعي تمام؟ اللي فيها الضغط تبعي هنا هيك هي
+
+817
+00:59:11,600 --> 00:59:15,480
+هذه القيمة تقاطع درجة الحرارة اللي بديها مع الضغط
+
+818
+00:59:15,480 --> 00:59:18,480
+اللي .. اللي بديها هي هذه قيمة ال Z factor اللي
+
+819
+00:59:18,480 --> 00:59:22,300
+بروح ب .. ب .. أطبق فيهاتمام هي هذه قيمة ال z
+
+820
+00:59:22,300 --> 00:59:26,060
+factor اللي بروح بطبق فيها و بحسب حسابات تبعتي زي
+
+821
+00:59:26,060 --> 00:59:29,820
+ما حكينا لو انا عديت التجربة مرة مرتين تلاتة اربع
+
+822
+00:59:29,820 --> 00:59:34,900
+عشرة و حصلت على مثلا عشر قيم باخد ال average تبعهم
+
+823
+00:59:34,900 --> 00:59:39,100
+بدربهم بال z factor بحصل على ال volume و بطبق في
+
+824
+00:59:39,100 --> 00:59:43,180
+القوانين تبعتي القيمة اللي انا جستها على القيمة
+
+825
+00:59:43,180 --> 00:59:46,570
+اللي المفروض تطلعضرب 100% بحسب ال accuracy بحسب ال
+
+826
+00:59:46,570 --> 00:59:50,450
+coefficient of variation بشوف نسبة دقة و صحة ال ..
+
+827
+00:59:50,450 --> 00:59:54,070
+ال vibe بتاعي و بحكم على شغلها و هل انها محتاجة
+
+828
+00:59:54,070 --> 01:00:00,690
+انها يتم تصليحها او انه لأ مناسبة و تستخدم يعني
+
+829
+01:00:00,690 --> 01:00:05,350
+موثوقة للاستخدام تمام؟بهيك بنكون خلصنا محاضرتنا
+
+830
+01:00:05,350 --> 01:00:10,030
+لليوم ان شاء الله تكون هيك الاشياء واضحة حولت ان
+
+831
+01:00:10,030 --> 01:00:15,350
+انا اورجيكم صور اورجيكم فيديو لكيف ال by بتاعي كنت
+
+832
+01:00:15,350 --> 01:00:18,810
+نستخدمها و تعليمات اللي بنتابعها أثناء ال by
+
+833
+01:00:18,810 --> 01:00:25,490
+bettingلعسى انه يعني تكون الاشي مش مبهم لقلكم لإنه
+
+834
+01:00:25,490 --> 01:00:29,810
+يعني احنا مقدرين انه بداية التخصص لازم تكون ..
+
+835
+01:00:29,810 --> 01:00:35,650
+تكون يعني كل إشي سهل ومستثار لحتى تتمكنوا من الفهم
+
+836
+01:00:35,650 --> 01:00:40,430
+المعلومة وإدراكها يعطيكم ألف عافية وشكرا لكم
+
diff --git a/PL9fwy3NUQKwY9jl8xw-bWkVZkWcGGyspd/S5RQk6QZXf4_raw.srt b/PL9fwy3NUQKwY9jl8xw-bWkVZkWcGGyspd/S5RQk6QZXf4_raw.srt
new file mode 100644
index 0000000000000000000000000000000000000000..b1948ca3170a9a05df25fb1852b014e8ddcebc32
--- /dev/null
+++ b/PL9fwy3NUQKwY9jl8xw-bWkVZkWcGGyspd/S5RQk6QZXf4_raw.srt
@@ -0,0 +1,3360 @@
+1
+00:00:04,520 --> 00:00:07,580
+السلام عليكم ورحمة الله وبركاته طالبتي اليوم ان
+
+2
+00:00:07,580 --> 00:00:12,060
+شاء الله رح ناخد معملنا رقم اتنين بعنوان automatic
+
+3
+00:00:12,060 --> 00:00:15,600
+by-produce and calibration معمل رقم اتنين فيه مساق
+
+4
+00:00:15,600 --> 00:00:20,760
+مقدمة في التحليل الطبية طبعا هذا كان اول موضوع كان
+
+5
+00:00:20,760 --> 00:00:25,100
+المفروض ان احنا ناخده عملي و نشتغل بادانا ولكن
+
+6
+00:00:25,100 --> 00:00:30,440
+للأسف نظرا لزروف المحيطة فيناراح ناخد ان شاء الله
+
+7
+00:00:30,440 --> 00:00:33,660
+.. راح ناخد النظري تبعه و ان شاء الله بعودتنا
+
+8
+00:00:33,660 --> 00:00:38,460
+للمختبرات بناخد التطبيق العملي لإله فاليوم ان شاء
+
+9
+00:00:38,460 --> 00:00:41,060
+الله موضوعنا عن ال automatic by bit use and
+
+10
+00:00:41,060 --> 00:00:44,280
+calibration خلينا نشوف إيش يعني automatic by bit
+
+11
+00:00:44,280 --> 00:00:47,480
+هلأ احنا المحاضرة الماضية خلينا بس نحكي هالنقطة
+
+12
+00:00:47,790 --> 00:00:53,150
+المحاضرة الماضية كانت بتحكي عن ال .. ال .. ال
+
+13
+00:00:53,150 --> 00:00:58,270
+volumes و الحجوم و المحاليل و كيف أنا بتعامل مع
+
+14
+00:00:58,270 --> 00:01:01,110
+المحاليل هاي و إيش أنواع المحاليل اللي تستخدم في
+
+15
+00:01:01,110 --> 00:01:05,930
+ال medical laboratories و .. و كيف أنا أخسف هاي
+
+16
+00:01:05,930 --> 00:01:10,690
+المحاليل، تمام؟طيب لما انا .. انا بدي أجي أنقل
+
+17
+00:01:10,690 --> 00:01:13,950
+المحلول من إلى يعني مثلا انا عندي عينة بدي
+
+18
+00:01:13,950 --> 00:01:17,970
+أضيفيلها reagent علشان أعمل فحص معين أو بدي أاخد
+
+19
+00:01:17,970 --> 00:01:22,130
+.. بدي أنقل حجوم علشان أعمل dilution زي ما أخدنا
+
+20
+00:01:22,130 --> 00:01:25,800
+ال .. مسائل ال dilution المحاضرة الماضيةكيف أنا
+
+21
+00:01:25,800 --> 00:01:29,140
+بدي أنقلها؟ هنا بال medical laboratories
+
+22
+00:01:29,140 --> 00:01:32,660
+بالمختبرات الطبية احنا بنستخدم اشي اسمه automatic
+
+23
+00:01:32,660 --> 00:01:37,100
+pipet اللي هو الأداة اللي موجودة بهذه الصورة اسمها
+
+24
+00:01:37,100 --> 00:01:40,740
+automatic pipet خلينا نشوف اش يعني automatic pipet
+
+25
+00:01:41,150 --> 00:01:45,250
+هلأ automatic pipet هي عبارة عن أداة تستخدم في ال
+
+26
+00:01:45,250 --> 00:01:48,110
+biological و ال biochemical و ال microbiological
+
+27
+00:01:48,110 --> 00:01:52,310
+labs سواء كانت لل teaching purposes أو لل research
+
+28
+00:01:52,310 --> 00:01:56,490
+purposes، تمام؟ يعني تستخدم في مختبرات الأحياء و
+
+29
+00:01:56,490 --> 00:01:59,750
+مختبرات ال biochemistry و ال microbiology في كل
+
+30
+00:01:59,750 --> 00:02:04,700
+أقسام المختبرات الطبيةوتستخدم إما للتدريس أو حتى
+
+31
+00:02:04,700 --> 00:02:09,640
+للعمل لممارسة المهنة أو لأغراض البحث العلمى طبعا
+
+32
+00:02:09,640 --> 00:02:13,700
+من شأنهاي ال bybit أنها تعملى accurate transfer of
+
+33
+00:02:13,700 --> 00:02:18,500
+volume يعني أنها تنقل الحجوم بشكل دقيق جدا بمثاق
+
+34
+00:02:18,500 --> 00:02:22,050
+الكيميا يمكن أخدتوا معامل كيميا قبل هكو كنتوا
+
+35
+00:02:22,050 --> 00:02:25,250
+تستخدموا بايبتات اللي هي زي ال glass pipe اللي كان
+
+36
+00:02:25,250 --> 00:02:29,490
+عبارة عن بايبت glass أداة زجاجية و في آخرها أو في
+
+37
+00:02:29,490 --> 00:02:33,210
+نهايتها بيكون في عندي مستودع من المطاط انا بتحكم
+
+38
+00:02:33,210 --> 00:02:37,160
+بدخول و خروج الهواء و دخول و خروج السائلالى ال by
+
+39
+00:02:37,160 --> 00:02:41,280
+bit طبعا هاي ال glass by bit بتستخدم بال chemical
+
+40
+00:02:41,280 --> 00:02:45,380
+laboratory ولكن للأسف هي not accurate و not
+
+41
+00:02:45,380 --> 00:02:49,820
+precise مش accurate و مش precise متى؟ طبعا لما بدي
+
+42
+00:02:49,820 --> 00:02:53,640
+أنقل حجوم أقل من one ml و احنا هنا بالمختبرات
+
+43
+00:02:53,640 --> 00:02:57,000
+الطبية بنتعامل دائما مع حجوم صغيرة جدا بال
+
+44
+00:02:57,000 --> 00:03:01,970
+microliter يعني أقل من one mlفبالتالي انا بدي أداة
+
+45
+00:03:01,970 --> 00:03:07,230
+تنقل الحجم هذا الصغير او الحجم القليل بطريقة more
+
+46
+00:03:07,230 --> 00:03:11,170
+accurate and more precise how باستخدام ال
+
+47
+00:03:11,170 --> 00:03:15,470
+automatic pipet اذا ال glass pipet هي تستخدم بال
+
+48
+00:03:15,470 --> 00:03:19,670
+chemical laboratory ولكن للأسف في الحجوم الصغيرة
+
+49
+00:03:19,670 --> 00:03:25,060
+not accurate not precise انا محتاجة أداة تكونأكثر
+
+50
+00:03:25,060 --> 00:03:28,560
+دقة more accurate more precise لهيك أنا بستخدم ال
+
+51
+00:03:28,560 --> 00:03:32,200
+automatic by bit لإن كل شغلي بيكون على ال volumes
+
+52
+00:03:32,200 --> 00:03:37,480
+اللي أقل من one ml طبعا طيب كل by bit من هدول ال
+
+53
+00:03:37,480 --> 00:03:42,440
+automatic by bit مصممة إن هي تنقل يعني مدى أو
+
+54
+00:03:42,440 --> 00:03:47,050
+range من الحجوم من إلىيعني في بايبت متكون مصممة
+
+55
+00:03:47,050 --> 00:03:50,930
+تنقل من واحد microliter لعشرة microliter في من ..
+
+56
+00:03:50,930 --> 00:03:54,770
+مصممة بتكون من عشرة microliter لمية microliter في
+
+57
+00:03:54,770 --> 00:03:58,610
+من مية microliter ل one ml تمام ف .. و في من one
+
+58
+00:03:58,610 --> 00:04:04,050
+ml لخمسة ml فإذا ال .. في بايبتات أنواع و حجوم و
+
+59
+00:04:04,050 --> 00:04:08,390
+كل بايبت بتكون إلها range يعني مدى من إلى
+
+60
+00:04:08,390 --> 00:04:15,140
+بتستخدموا في ال .. ال .. ال ..نقل الحجوم هذه و
+
+61
+00:04:15,140 --> 00:04:19,600
+طبعا احنا المفروض احنا مانتخطى هذا ال .. ال .. ال
+
+62
+00:04:19,600 --> 00:04:23,820
+range تبعها يعني لازم انا مثلا اذا بدي انقل خمسين
+
+63
+00:04:23,820 --> 00:04:28,320
+microliter يفضل ان انا اخد بايبت ال range تبعها
+
+64
+00:04:28,320 --> 00:04:31,480
+بحيث انه الخمسين تكون واقعة فيه ال range في داخل
+
+65
+00:04:31,480 --> 00:04:34,980
+هذا ال range يعني مثلا باخد بايبتبتنقل من عشرة
+
+66
+00:04:34,980 --> 00:04:38,420
+microliter لمية microliter ال volume رقم خمسين
+
+67
+00:04:38,420 --> 00:04:42,580
+هيكون already موجود يعني في المدى و ال by بتحتقدر
+
+68
+00:04:42,580 --> 00:04:46,880
+انها تنقلي هذا ال volume فبالتالي انا هكون ضمنت ال
+
+69
+00:04:46,880 --> 00:04:50,250
+accuracy و ال precision لناقل ال volumesطيب إذا
+
+70
+00:04:50,250 --> 00:04:54,850
+إحنا نوّهنا إنه أنا في عندي أنواع وحجوم ويعني قدرة
+
+71
+00:04:54,850 --> 00:04:58,930
+استيعابية لال buy bits يعني مش كلها واحد مش كلها
+
+72
+00:04:58,930 --> 00:05:03,930
+مصممة إنها تنقلني نفس الحجم طيب فإذا في عندي حاجة
+
+73
+00:05:03,930 --> 00:05:08,010
+اسمها buy bit man model طبعا هي سبع أنواع من ال
+
+74
+00:05:08,010 --> 00:05:12,440
+buy bits مصممة إنها تنقل حجوم بطريقة مختلفةطبعا
+
+75
+00:05:12,440 --> 00:05:17,560
+هذه ال .. ال man model يعني لو هم السبعة بيكونوا
+
+76
+00:05:17,560 --> 00:05:20,980
+بينقلولي من point one microliter يعني من اقل من
+
+77
+00:05:20,980 --> 00:05:24,680
+واحد microliter لحد خمس تلاف microliter يعني لحد
+
+78
+00:05:24,680 --> 00:05:30,100
+خمس ML يعني في تنوع من ال by بتات هذه و هلأ بنشوف
+
+79
+00:05:30,100 --> 00:05:35,090
+بالجدوة اللي في ال slide ال ..التالية هيوضحلي إيش
+
+80
+00:05:35,090 --> 00:05:38,970
+يعني by bit مع الموديل ولكن هنا بيحكيلنا إنه the
+
+81
+00:05:38,970 --> 00:05:42,710
+most often used يعني الأكثر استخداما هي ال by
+
+82
+00:05:42,710 --> 00:05:49,150
+bitات اللي تسمى ال B20 و ال B200 و ال B1000 هدول
+
+83
+00:05:49,150 --> 00:05:54,150
+ال models هم الأكثر استخداما اللي بينقلولي حجم من
+
+84
+00:05:54,150 --> 00:05:58,450
+اتنين لعشرينو من خمسين ل متين و من مية ل ألف
+
+85
+00:05:58,450 --> 00:06:02,170
+microliter تمام هاي المدى تبعهم respectively مرتب
+
+86
+00:06:02,170 --> 00:06:07,410
+بالترتيب ال B20 بتنقل من اتنين ل عشرين microliter
+
+87
+00:06:07,410 --> 00:06:12,990
+تمام و ال بايبت B20 بتنقل volume من خمسين ل متين
+
+88
+00:06:12,990 --> 00:06:16,730
+microliter و البايبت الف بتاخد من مية ل الف
+
+89
+00:06:16,730 --> 00:06:20,970
+microliter يعني الرقم اللي جنب ال B تمام بي يعني
+
+90
+00:06:20,970 --> 00:06:25,240
+بايبت و الرقم هذا يعني أقصى قيمة بتقدر تاخدهاطبعا
+
+91
+00:06:25,240 --> 00:06:28,800
+ومش .. مش أدنى قيمة لأ فى أدنى .. الأدنى قيمة غير
+
+92
+00:06:28,800 --> 00:06:32,080
+مذكورة هلأ نيجى نشوف ال buy bet man model هى فى
+
+93
+00:06:32,080 --> 00:06:36,960
+عندى واحد اتنين أربع ستة سبع أنواع من ال buy bet
+
+94
+00:06:36,960 --> 00:06:43,870
+طبعا ومسمى بي تو يعنى بتنقل من zero لتنين طبعاو ال
+
+95
+00:06:43,870 --> 00:06:51,010
+B20 من 0 ل 10 بتاخد ال B20 بتاخد من 0 ل 20 ال B100
+
+96
+00:06:51,010 --> 00:06:55,250
+بتاخد من 0 ل 100 و 200 من 0 ل 200 و هكذا من 0 ل
+
+97
+00:06:55,250 --> 00:07:00,570
+1000 يعني من 1 أمال و هنا من 0 ل 5000 لحد one ..
+
+98
+00:07:00,570 --> 00:07:04,310
+لحد خمس أمال هذا ال range بصي بالهذا اللي بيكون
+
+99
+00:07:04,310 --> 00:07:07,690
+مسجل على ال by بت نفسها يعني بيكون .. بيكون ..
+
+100
+00:07:07,690 --> 00:07:12,960
+بيعطين ال range انه من .. من كذا لكذاأنا لما بدى
+
+101
+00:07:12,960 --> 00:07:16,040
+أستخدم ال by bet هذه طبعا انا مابستخدم ال by bet
+
+102
+00:07:16,040 --> 00:07:18,700
+عشان أنقله zero volume يعني انا مابديش أنقل volume
+
+103
+00:07:18,700 --> 00:07:21,840
+ليش أستخدم ال by bet؟ zero volume يعني no volume
+
+104
+00:07:21,840 --> 00:07:26,160
+طبعا فإذا في عندي حاجة اسمها recommended range
+
+105
+00:07:26,160 --> 00:07:29,600
+يعني ال range الموصفة يعني أنا بستخدم ال by bet
+
+106
+00:07:29,600 --> 00:07:36,260
+لحتى انه انا يعني في range مسموحلي فيه ايش هو اللي
+
+107
+00:07:36,260 --> 00:07:42,040
+مسموحلي فيه؟ من point واحد لاتنين طبعاو ال .. و
+
+108
+00:07:42,040 --> 00:07:45,880
+هنا من نص لعشرة من اتنين لعشرين من عشرة لمية و
+
+109
+00:07:45,880 --> 00:07:50,400
+هكذا من خمسين لمتان طيب هذه ال by bet اللي من
+
+110
+00:07:50,400 --> 00:07:53,780
+خمسين لمتان ممكن تسحب لأقل من خمسين اه ممكن تسحب
+
+111
+00:07:53,780 --> 00:07:56,560
+لأقل من خمسين ال range تبعها اصلا من zero لمتان
+
+112
+00:07:56,900 --> 00:08:00,800
+ولكن هو اذا سحبت اقل من خمسين فهي بتكون inaccurate
+
+113
+00:08:00,800 --> 00:08:05,200
+and imprecise يعني مش accurate ومش precise فانا
+
+114
+00:08:05,200 --> 00:08:08,300
+يفضل ان انا استخ .. اخت .. يعني اتبع ال
+
+115
+00:08:08,300 --> 00:08:13,460
+recommended range علشان أستخ .. أختار ال .. ال ..
+
+116
+00:08:13,460 --> 00:08:18,170
+ضيبة المناسبة لإلي تمام؟طيب يعني مثلا لو أنا بدي
+
+117
+00:08:18,170 --> 00:08:23,030
+أنقل خمسين microliter تمام؟ خمسين microliter مين
+
+118
+00:08:23,030 --> 00:08:29,190
+أفضل أختار ال .. ال by بت مثلا رقم ال B100 ولا ال
+
+119
+00:08:29,190 --> 00:08:33,410
+B200؟ يفضل أن أنا أختار ال B100 لإنه الخمسين
+
+120
+00:08:33,410 --> 00:08:36,570
+القيمة خمسين تقع في نص ال range يعني أنا ب .. بكون
+
+121
+00:08:36,570 --> 00:08:41,710
+ضمنة إنه مافيش أخطاءتمام ال by بت على ال border
+
+122
+00:08:41,710 --> 00:08:45,850
+على الحدود تمام ممكن يكون فيها نسبة خطأ لكن في
+
+123
+00:08:45,850 --> 00:08:49,950
+منتصف ال range بتكون دقيقة فإذا انا بفضل ان انا
+
+124
+00:08:49,950 --> 00:08:54,810
+لما بدي اختار اللي بدي انقل volume بختار ال by بت
+
+125
+00:08:54,810 --> 00:08:57,870
+اللي هذا ال volume يتوسط ال range تبعها او موجود
+
+126
+00:08:57,870 --> 00:09:02,120
+في ال range تبعهاطبعا طيب هلأ لو بدي أنقل مثلا ميت
+
+127
+00:09:02,120 --> 00:09:08,300
+microliter مين أفضل أختار ال B100 طبعا ولا ال B200
+
+128
+00:09:08,300 --> 00:09:15,620
+ولا ال B1000 يفضل أن أنا أختار ال B200 طبعا و ممكن
+
+129
+00:09:15,620 --> 00:09:18,560
+أنا أختار ال B100 بتكون دقيقة يعني ال higher range
+
+130
+00:09:18,560 --> 00:09:23,040
+تبعتها بتكون أدق دايما طبعا ال higher range بتكون
+
+131
+00:09:23,040 --> 00:09:26,680
+أدق من ال lower range لل bybit طبعا يعني هذه ال
+
+132
+00:09:26,680 --> 00:09:32,120
+bybit ال B100يعني لو بدأ أنقل 100 microliter من ال
+
+133
+00:09:32,120 --> 00:09:37,620
+volume ممكن أستخدم ال B200 ال .. ال .. ال B100
+
+134
+00:09:37,620 --> 00:09:43,440
+ممكن أستخدم ال B1000 تمام؟ و .. لكن مثلا هذه ال by
+
+135
+00:09:43,440 --> 00:09:47,500
+bit اللي هو ال range تبعها من عشرة لمية تمام؟ عند
+
+136
+00:09:47,500 --> 00:09:50,800
+المية بتكون أضاق من من .. من .. من أني أسحب فيها
+
+137
+00:09:50,800 --> 00:09:54,640
+عشرة microliter يعني دايما ال higher limit تبعتها
+
+138
+00:09:54,640 --> 00:09:57,990
+بتكون أضاق من ال lower limit لل by bit نفسهاطبعا
+
+139
+00:09:57,990 --> 00:10:02,750
+إذا نرجع نعيد الصياغة تاني لو أنا في عندى .. بدي
+
+140
+00:10:02,750 --> 00:10:05,690
+أنقل 100 microliter مين أفضل أستخدم؟ الأفضل أن أنا
+
+141
+00:10:05,690 --> 00:10:09,990
+أستخدم ال B200 لإنها المية تتوسط ال range ممكن
+
+142
+00:10:09,990 --> 00:10:13,730
+أستخدم ال B100 لإنه حكينا ال higher limit بتكون
+
+143
+00:10:13,730 --> 00:10:16,990
+أدق دايما من ال lower limit لل by bit ولكن أنا
+
+144
+00:10:16,990 --> 00:10:20,110
+مابستخدم .. يعني ممكن أستخدمها بس مابكنش دقيقة و
+
+145
+00:10:20,110 --> 00:10:24,170
+.. و .. كتير لإنه دايما حكينا إنه ال lower limit
+
+146
+00:10:24,170 --> 00:10:27,370
+لل by bit بتكون أقل دقة من ال higher limitهذا
+
+147
+00:10:27,370 --> 00:10:31,690
+بالنسبة ل .. ال .. ال .. ال by bit man model حكينا
+
+148
+00:10:31,690 --> 00:10:36,770
+مين هم اكتر شيوعا و اكتر استخداما بي twenty و ال
+
+149
+00:10:36,770 --> 00:10:41,910
+by one hundred و ال by two hundred تمام؟ اذا ال by
+
+150
+00:10:41,910 --> 00:10:46,570
+عشرين ال by مية و ال by متين هم اكتر ال .. ال by
+
+151
+00:10:46,570 --> 00:10:50,570
+بتات شيوعا و استخداماتمام طيب هلا بنا نحكي عن
+
+152
+00:10:50,570 --> 00:10:56,150
+أنواع .. يعني sorry مكونات البيبت و أجزاقها طيب
+
+153
+00:10:56,150 --> 00:11:00,430
+البيبت زي ما انتوا شايفين بهذا الشكل مكونة من حاجة
+
+154
+00:11:00,430 --> 00:11:05,030
+اسمها ال .. البش .. البشبطن أو البلنجر نحكي عنه
+
+155
+00:11:05,030 --> 00:11:09,770
+إما بشبطن أو بلنجر إيش يعني بشبطن أو بلنجر؟زي ما
+
+156
+00:11:09,770 --> 00:11:13,250
+انتوا شايفين هذا ال push button هو عبارة عن كبسة
+
+157
+00:11:13,250 --> 00:11:18,830
+push انا ممكن انا اضغط عليها و تطلع و تنزل من ال
+
+158
+00:11:18,830 --> 00:11:24,030
+by button تمام؟ و هذا الرد تبعها طبعا هذا الرد ال
+
+159
+00:11:24,030 --> 00:11:29,980
+.. ال .. ال .. ال ..القضيب هذا ممكن انه يلتف دائري
+
+160
+00:11:29,980 --> 00:11:35,500
+علشان يتحكم في ال volume تمام؟ يتحكم في ال volume
+
+161
+00:11:35,500 --> 00:11:39,620
+اللي موجودة على ال digital screen هنا تمام؟ هلأ في
+
+162
+00:11:39,620 --> 00:11:45,240
+هنا adapter موجود هذا يعني علشان انا اقلل من حركة
+
+163
+00:11:45,240 --> 00:11:47,800
+ال vibe يعني ممكن انا اسبط هذا ال adapter على
+
+164
+00:11:47,800 --> 00:11:51,880
+أصبعي .. على أصبعي السبابة وامسكها بشكل انه يكون
+
+165
+00:11:51,880 --> 00:11:58,230
+انا متحكمة فيها ومسكاها بشكل صحيحهذا بسميه tip
+
+166
+00:11:58,230 --> 00:12:04,410
+eject .. tip ejector أو tip eject button تمام؟ هو
+
+167
+00:12:04,410 --> 00:12:09,730
+عبارة عن كبسة بتتخلص من ال tip يعني بتتحرك .. مجرد
+
+168
+00:12:09,730 --> 00:12:14,360
+ما أضغط عليها ال tip اللي هنا بتفكطبعا هذا عبارة
+
+169
+00:12:14,360 --> 00:12:19,020
+عن ال tip ejector و هنا tip holder و زي ما انتوا
+
+170
+00:12:19,020 --> 00:12:23,560
+شايفين هنا في مكان لأ ال handle من هنا بمسكها و
+
+171
+00:12:23,560 --> 00:12:29,220
+هنا الشاشة اللي بتكون موجود عليها القراءة بتكون
+
+172
+00:12:29,220 --> 00:12:35,070
+موجود عليها القراءة تبعتيطيب، هلأ بالنسبة لل .. ال
+
+173
+00:12:35,070 --> 00:12:39,110
+.. بايبت هي بتيجي تمام لحالها هيك و فيه انا عندي
+
+174
+00:12:39,110 --> 00:12:43,370
+حاجة اسمها tips او بايبت tip طبعا هذه ال .. بايبت
+
+175
+00:12:43,370 --> 00:12:48,400
+tip هي عبارة عن قطع بلاستيكيةهذه زي ما انتوا
+
+176
+00:12:48,400 --> 00:12:54,520
+شايفين في الصورة هنا هي عبارة عن قطع بلاستيكية لت
+
+177
+00:12:54,520 --> 00:12:59,980
+.. ت .. يعني بتتركب على ال .. تستخدم لبيبتات و هي
+
+178
+00:12:59,980 --> 00:13:02,960
+تتراوح في الأحجام تبعتها يعني البيبتات الصغيرة
+
+179
+00:13:02,960 --> 00:13:06,900
+بيكون إلها تبات، بيكون إلها تب صغيرة، البيبتات
+
+180
+00:13:06,900 --> 00:13:11,630
+الأكبر بيكون إلها تب أكبر و هكذاهي قطع بلاستيكية و
+
+181
+00:13:11,630 --> 00:13:16,170
+هذه القطع البلاستيكية بستخدمها انا one use ايش
+
+182
+00:13:16,170 --> 00:13:20,090
+يعني one use يعني بستستخدم لمرة واحدة فقط لنقل حجم
+
+183
+00:13:20,090 --> 00:13:24,210
+لمرة واحدة فقط و بعد هيك انا بتلفها بتخلص منها
+
+184
+00:13:24,210 --> 00:13:29,490
+طبعا فإذا مابرجع استخدمها مرة تانية فهذه هي التبات
+
+185
+00:13:29,490 --> 00:13:35,870
+او القطع اللي بتتركب على ال by bit وبيكون الحجم او
+
+186
+00:13:35,870 --> 00:13:39,550
+ال volume داخلها يعني انا السائل اللي انا بنقله
+
+187
+00:13:39,700 --> 00:13:44,480
+بيكون موجود داخل هاي التب وبيتم ال .. ال .. نقله
+
+188
+00:13:44,480 --> 00:13:48,760
+من مكان إلى آخر حسب أنا وين بدي أو كيف بدي أشتغل
+
+189
+00:13:48,760 --> 00:13:55,780
+طيب، إذا هاي مكونات البايبت و أجزاقها و يعرفنا إنه
+
+190
+00:13:55,780 --> 00:14:00,250
+التب هي قطعة خارجيةتتركب لل by bet مش ثابتة في ال
+
+191
+00:14:00,250 --> 00:14:05,490
+by bet و انه هذه القطعة ايضا ممكن اني استخدمها
+
+192
+00:14:05,490 --> 00:14:09,210
+لمرة واحدة فقط يعني بتلفها بعد ما استخدمها خلاص
+
+193
+00:14:09,210 --> 00:14:13,430
+برميها و اذا هذه يطلق عليها مصطلح disposable
+
+194
+00:14:13,430 --> 00:14:19,040
+disposable يعني استخدام لمرة واحدة فقطوحكينا ايضا
+
+195
+00:14:19,040 --> 00:14:24,320
+انه هذه ال .. ال .. التب يعني بتتنقل .. بيكون
+
+196
+00:14:24,320 --> 00:14:27,980
+حجمها متناسب مع ال bybit ال by بتاعة اللي بتنقل
+
+197
+00:14:27,980 --> 00:14:31,780
+حجوم صغيرة بيكون لها تب صغيرة و ال by بتاعة اللي
+
+198
+00:14:31,780 --> 00:14:37,440
+بتنقل حجم أكبر بيكون لها تب حجمه أكبر نوعا ماطيب
+
+199
+00:14:37,440 --> 00:14:40,980
+فيه هنا نصائح لاستخدام ال micro bibit اللي هي
+
+200
+00:14:40,980 --> 00:14:45,120
+عبارة عن إيش هذه النصائح؟ أول حاجة never exceed
+
+201
+00:14:45,120 --> 00:14:49,140
+the upper or lower limit of this bibit يعني أنه
+
+202
+00:14:49,140 --> 00:14:55,710
+يعني مثلا هاي ال bibit هايبتنقللي مثلا من عشرة
+
+203
+00:14:55,710 --> 00:15:00,350
+لمية، يعني ممنوع أنا أسحب أقل من عشرة و ممنوع أسحب
+
+204
+00:15:00,350 --> 00:15:04,590
+فيها أكتر من مية، يعني ما أسحب أقل من عشرة ولا
+
+205
+00:15:04,590 --> 00:15:09,350
+أكتر من مية، يعني ما أتخطأ ال range هادا، إما بأقل
+
+206
+00:15:09,350 --> 00:15:13,840
+منه أو بأكتر منههذا معناه الجملة هاى الجملة هاى
+
+207
+00:15:13,840 --> 00:15:18,300
+معناها انه never exceed ما اتخطى ال upper او ال
+
+208
+00:15:18,300 --> 00:15:22,320
+lower limit لل range لل by bit ال by bit يعني
+
+209
+00:15:22,320 --> 00:15:28,180
+مصممة بأنها تنقلي داخل range معين فانا باحترم هذا
+
+210
+00:15:28,180 --> 00:15:32,880
+ال range بلتزم فيه ما بستخدمها ال by bit الاقل منه
+
+211
+00:15:32,880 --> 00:15:37,900
+ولا الاكثر منه على نقل حجوم علشان ما اكون بنقل
+
+212
+00:15:37,900 --> 00:15:42,840
+حجوم بطريقة مش precise و مش accurateتمام طيب so
+
+213
+00:15:42,840 --> 00:15:46,840
+the desired volume by turning centrally located
+
+214
+00:15:46,840 --> 00:15:49,700
+ring clockwise to increase volume or
+
+215
+00:15:49,700 --> 00:15:53,120
+counterclockwise to decrease احنا حكينا انه هذا
+
+216
+00:15:53,120 --> 00:15:59,290
+القضيب او ال .. ال .. ال ..الرد تمام هذا بيتحرك
+
+217
+00:15:59,290 --> 00:16:02,530
+بشكل دائري و من خلاله انا بتحكم بالحجم اللى موجود
+
+218
+00:16:02,530 --> 00:16:06,290
+على الشاشة هنا تمام يعني من خلاله انا بحدد الحجم
+
+219
+00:16:06,290 --> 00:16:11,450
+اللى بديها بحكيلك هنا انه تحركيه ال ال ال
+
+220
+00:16:11,450 --> 00:16:18,530
+clockwise تمام و عشان يزيد الحجم و بنحركه counter
+
+221
+00:16:18,530 --> 00:16:25,520
+clockwise to decrease ال volumeطبعا انا هنا بالشكل
+
+222
+00:16:25,520 --> 00:16:29,140
+او بالتجربة العملية لما بنشرح احنا كيف نستخدم ال
+
+223
+00:16:29,140 --> 00:16:32,720
+bybit في المعامل انا بخل ال bybit .. بخل الطالبة
+
+224
+00:16:32,720 --> 00:16:37,120
+تمسك ال bybit تمام و بحيث انه ال digital screen
+
+225
+00:16:37,120 --> 00:16:41,300
+تكون مواجهةلإلها تمام يعني تكون عينها على ال
+
+226
+00:16:41,300 --> 00:16:45,800
+digital screen وبالتالي بما أنه بيكون عينها على ال
+
+227
+00:16:45,800 --> 00:16:49,660
+digital screen إذا حركت هذا الرد بشكل دائري
+
+228
+00:16:49,660 --> 00:16:54,260
+باتجاهها يعني لعندها تمام هيك بيزيد ال volume أما
+
+229
+00:16:54,260 --> 00:16:59,180
+إذا حركته باتجاه المعاكس يعني بعيد عنها فهو بيقل
+
+230
+00:16:59,180 --> 00:17:02,340
+ال volume تمام هذا بده ممارسة ان شاء الله بنشرحه
+
+231
+00:17:02,340 --> 00:17:09,020
+بالطريقة أو بالتجربة العمليةتمام؟ هذا بالنسبة ل ..
+
+232
+00:17:09,020 --> 00:17:14,320
+ال .. ال .. ال by bet و الطرق أو اللي هي الخطوات
+
+233
+00:17:14,320 --> 00:17:17,740
+أو اللي .. ال .. التعليمات اللي لازم أنا ألتزم
+
+234
+00:17:17,740 --> 00:17:22,920
+فيها أثناء السحب أو نقل الحجوم باستخدام ال by bet
+
+235
+00:17:23,850 --> 00:17:29,330
+في شغل هنا انه ال .. ال by bit هنا طبعا هي بتكون
+
+236
+00:17:29,330 --> 00:17:33,210
+مصممة انها تنقلي او تقفز قفزات في ال volume يعني
+
+237
+00:17:33,210 --> 00:17:38,970
+مثلا هاي هنا بحكيلي انه هي صح يعني من خمسين ل متين
+
+238
+00:17:38,970 --> 00:17:45,210
+بتسحب لكن انه مثلا انا لما بدي ..أحرك بصير بحرك
+
+239
+00:17:45,210 --> 00:17:48,450
+بيزيد ال volume كل شوية point اتنين point اتنين
+
+240
+00:17:48,450 --> 00:17:51,890
+لحد ما اصل لل volume اللي بديها لذا هذه هي ال ..
+
+241
+00:17:51,890 --> 00:17:55,450
+ال .. مدى التحريك او اللي هي القافزات في ال
+
+242
+00:17:55,450 --> 00:18:01,330
+volumes طبعا طيب بليت ا tip place a tip on the
+
+243
+00:18:01,330 --> 00:18:05,230
+discharge end of the pipet طبعا انا ب .. بركب ال
+
+244
+00:18:05,230 --> 00:18:10,370
+tip في المكان المخصص لإلها و بعد هيك طبعا ب ..
+
+245
+00:18:12,600 --> 00:18:16,600
+باستخدام البلانجر ب .. باستخدمه علشان اسحب ال
+
+246
+00:18:16,600 --> 00:18:20,520
+volumes هذا البلانجر اللي احنا شايفينه تمام مش ما
+
+247
+00:18:20,520 --> 00:18:24,180
+.. ايضا هو يعني فايد .. فايديته مش بس انه متصل
+
+248
+00:18:24,180 --> 00:18:28,360
+بالرد high بال .. و بتحكم بال volume لأ هذا
+
+249
+00:18:28,360 --> 00:18:31,600
+البلانجر احنا كتركم push button يعني انا ممكن اكدس
+
+250
+00:18:31,600 --> 00:18:35,480
+عليا بيطلع و بينزلطبعا انا هذا لما بدي أكبس عليه و
+
+251
+00:18:35,480 --> 00:18:39,860
+يطلع و ينزل بينزل عند ضغطتين، الضغطة الأولى لما
+
+252
+00:18:39,860 --> 00:18:43,840
+أضغط الضغطة الأولى بيقللي ال exact volume اللي
+
+253
+00:18:43,840 --> 00:18:50,720
+موجود ع الشاشة تمام؟ لما أضغط ضغطتين تمام؟بينقلي
+
+254
+00:18:50,720 --> 00:18:54,280
+extra volume ممكن يكون حجم مضاعف او extra volume
+
+255
+00:18:54,280 --> 00:18:59,560
+من ال volume اللي مصمم او موجود على الشاشة تمام؟
+
+256
+00:18:59,560 --> 00:19:04,400
+اذا هذا ال bush هذا البلنجر عبارة عن كبسة فيها two
+
+257
+00:19:04,400 --> 00:19:07,680
+stops يعني انا لما اضغط اول ضغطة بلاحظ انه خلاص
+
+258
+00:19:07,680 --> 00:19:12,780
+وجه اصبعي لدرجة معينة وانا بضغط لو ضغطت بقوة اكبر
+
+259
+00:19:12,780 --> 00:19:16,910
+ممكن اضغط الضغطة التانية يعني بنزل كمان مرةفالضغطة
+
+260
+00:19:16,910 --> 00:19:21,410
+الأولى لما أنا بكون ضغطاها بتكون ال by بتها تسحبلي
+
+261
+00:19:21,410 --> 00:19:25,750
+volume نفس ال volume اللى موجودة ع الشاشة لكن لو
+
+262
+00:19:25,750 --> 00:19:29,690
+أنا بدى أسحب two volumes او يعني بصري بدى أسحب و
+
+263
+00:19:29,690 --> 00:19:33,110
+أنا ضغطة ضغطتين هيسحبلي ال volume اللى موجودة ع
+
+264
+00:19:33,110 --> 00:19:37,610
+الشاشة وكمان extra volume تانى يعني volume بزيادة
+
+265
+00:19:37,610 --> 00:19:40,710
+طب انا ليش امتى بستخدم الضغطة الأولى و امتى بستخدم
+
+266
+00:19:40,710 --> 00:19:45,100
+الضغطة التانية او الضغطتين هلأ بنشوف كدههي هنا
+
+267
+00:19:45,100 --> 00:19:47,920
+بيحكيلي انه البلانجر او اللي هو ال push button
+
+268
+00:19:47,920 --> 00:19:53,140
+تمام و ال stop at two different positions يعني هذا
+
+269
+00:19:53,140 --> 00:19:57,740
+البلانجر بيتوقف عند .. عند مكانين و انا لما اجي
+
+270
+00:19:57,740 --> 00:20:00,820
+اضغط عليه بلاحظ انه في عندي two resistance في
+
+271
+00:20:00,820 --> 00:20:05,020
+مقاومتين للضغطة تبعتي ال .. ال stop الأول او
+
+272
+00:20:05,020 --> 00:20:10,160
+الضغطة الأولى تمام و لو انا زودت ال .. الضغط تبعي
+
+273
+00:20:10,160 --> 00:20:16,630
+بنزل كمان ضغطة تانية فبالتاليال .. ال .. ال first
+
+274
+00:20:16,630 --> 00:20:21,870
+stopping تمام عند ال initial resistant تمام عند
+
+275
+00:20:21,870 --> 00:20:27,610
+أول يعني stop عند أول إعاقة لضغطة تبعتي بيجف ال ..
+
+276
+00:20:27,610 --> 00:20:30,850
+ال plunger عندها بتكون هذه عبارة عن الضغطة الأولى
+
+277
+00:20:31,230 --> 00:20:37,590
+طبعا و ال .. و هذه معناها انه انا هسحب ايش ال
+
+278
+00:20:37,590 --> 00:20:40,810
+desired volume يعني انا هاخد ال volume اللي موجود
+
+279
+00:20:40,810 --> 00:20:47,720
+ع الشاشة exactly باخده طبعا ال second stoppingلما
+
+280
+00:20:47,720 --> 00:20:53,380
+يكون .. لما انا بدي اضغط على الضغط التاني يعني انا
+
+281
+00:20:53,380 --> 00:20:58,900
+بدي اظل نازلة في البلانجر ضغطين فساعتها انا بكون
+
+282
+00:20:58,900 --> 00:21:02,540
+تجاوزت ال initial resistance عند .. تجاوزت الإعاقة
+
+283
+00:21:02,540 --> 00:21:06,540
+الأولى و انزلت كمان مرة في ال .. في البلانجرطبعا
+
+284
+00:21:06,540 --> 00:21:12,600
+فهدى ال .. الاشي معناها انه ال by bit راح تسحبلي
+
+285
+00:21:12,600 --> 00:21:17,940
+volume extra طبعا هتسحبلي ال .. ال volume extra
+
+286
+00:21:17,940 --> 00:21:22,720
+ساعتها انا لما بلقل الحجم بفضيه بضغط ضغطة واحدة
+
+287
+00:21:22,950 --> 00:21:26,350
+هتنقل الحجم و هيضل في عندي extra volume موجود في
+
+288
+00:21:26,350 --> 00:21:31,130
+ال tip ممكن أتخلص منه تمام؟ اذا في عندي ال .. ال
+
+289
+00:21:31,130 --> 00:21:34,370
+.. بلانجر ال .. او ال .. ال push button بيجف عن
+
+290
+00:21:34,370 --> 00:21:38,430
+ضغطين، الدغطة الأولى بتن .. لما انا أكون ضغطاها و
+
+291
+00:21:38,430 --> 00:21:42,270
+أسحب حجم من السائل معناها أنا سحبت نفس الحجم اللي
+
+292
+00:21:42,270 --> 00:21:45,600
+موجودة على الشاشةلكن اذا انا كنت ضغطة ضغطتين و
+
+293
+00:21:45,600 --> 00:21:50,120
+سحبت حجم معناها انا سحبت الحجم مع extra تمام لما
+
+294
+00:21:50,120 --> 00:21:54,040
+اجي افضية بدي انتبه ان انا اضغط ضغطة واحدة فقط
+
+295
+00:21:54,040 --> 00:21:57,520
+افضي ال volume اللي من ضغطة واحدة لان ضغطة واحدة
+
+296
+00:21:57,520 --> 00:22:00,380
+يعني ايش ال desired volume يعني ال volume اللي
+
+297
+00:22:00,380 --> 00:22:04,580
+بديها و بيضل في عندي extra طبعا طب ليش الغلبة هذه
+
+298
+00:22:04,580 --> 00:22:07,880
+ليش ضغطة واحدة و ضغطتين و ليش انا اخد extra volume
+
+299
+00:22:08,030 --> 00:22:11,550
+علشان في عندي نوعين من ال by betting نوع اسمه
+
+300
+00:22:11,550 --> 00:22:16,450
+forward by betting و reverse by betting ايش يعني
+
+301
+00:22:16,450 --> 00:22:19,770
+forward by betting يعني انه انا افوت في المحلول
+
+302
+00:22:19,770 --> 00:22:23,870
+تمام و انا ضغطة ضغطة واحدة اخد الحجم اللي بدياه هو
+
+303
+00:22:23,870 --> 00:22:27,390
+نفس الحجم اللي موجود على الشاشة exactly بالضبط
+
+304
+00:22:27,390 --> 00:22:32,530
+بدون زيادة و بعد هيك اجي افضيه افضيه في المحلول في
+
+305
+00:22:32,530 --> 00:22:37,350
+الوعاء اللي انا بدي اشتغل فيه و بفضيه و بضل ضغطة
+
+306
+00:22:37,540 --> 00:22:40,020
+ممكن اضغط اكتر من مرة او ممكن اضغط اكتر من مرة او
+
+307
+00:22:40,020 --> 00:22:44,880
+ممكن اضغط اكتر من مرة او ممكن اضغط اكتر من مرة او
+
+308
+00:22:44,880 --> 00:22:48,820
+ممكن اضغط اكتر من مرة او ممكن اضغط اكتر من مرة او
+
+309
+00:22:48,820 --> 00:22:48,960
+ممكن اضغط اكتر من مرة او ممكن اضغط اكتر من مرة او
+
+310
+00:22:48,960 --> 00:22:49,180
+ممكن اضغط اكتر من مرة او ممكن اضغط اكتر من مرة او
+
+311
+00:22:49,180 --> 00:22:49,860
+ممكن اضغط اكتر من مرة او ممكن اضغط اكتر من مرة او
+
+312
+00:22:49,860 --> 00:22:52,000
+ممكن اضغط اكتر من مرة او ممكن اضغط اكتر من مرة او
+
+313
+00:22:52,000 --> 00:22:53,780
+ممكن اضغط اكتر من مرة اإذا إيش ال forward
+
+314
+00:22:53,780 --> 00:22:57,380
+bybiting؟ معناها .. معناها إن أنا أضغط ضغطة على ال
+
+315
+00:22:57,380 --> 00:23:01,680
+bybit بعدين أجي أس .. أ .. أنقل الحجم أاخد الحجم
+
+316
+00:23:01,680 --> 00:23:04,200
+اللي بدياه هو ال exact volume اللي موجود على
+
+317
+00:23:04,200 --> 00:23:08,120
+الشاشة و لما أجي أفضيه في الوعاء ممكن أضغط ضغط أو
+
+318
+00:23:08,120 --> 00:23:11,040
+ضغطتين عشان أتأكد إن أنا فضيته كله و إن أنا
+
+319
+00:23:11,040 --> 00:23:14,700
+استخدمت ال volume بالضبط هو نفس اللي موجود على
+
+320
+00:23:14,700 --> 00:23:17,720
+الشاشة تمام؟ مايكونش ضاي .. إيش يضاي العالق في ال
+
+321
+00:23:17,720 --> 00:23:23,070
+tip تمام؟ إذا ..هذا معناه ال forward وهذه الطريقة
+
+322
+00:23:23,070 --> 00:23:27,410
+تستخدم عشان انا لو بدى انقل حجوم زى مين زى ال
+
+323
+00:23:27,410 --> 00:23:31,450
+aqueous solution المحليل اللى أصلها مية ال buffers
+
+324
+00:23:31,450 --> 00:23:35,310
+ال acid ال bases كل الأشياء المحليل هذه اللى أصلها
+
+325
+00:23:35,310 --> 00:23:39,750
+مية بستخدم معاها طريقة ال forward by bettingأما
+
+326
+00:23:39,750 --> 00:23:43,390
+طريقة ال reverse by betting فهي عبارة عن انه انا
+
+327
+00:23:43,390 --> 00:23:47,990
+باخد larger volume of solution بصحب larger volume
+
+328
+00:23:47,990 --> 00:23:51,990
+of solution ايش يعني؟ يعني بصحب extra يعني معناها
+
+329
+00:23:51,990 --> 00:23:55,430
+انا لما باجي باخد ال by bet او بنقل في ال by bet
+
+330
+00:23:55,430 --> 00:24:00,870
+بكون ضغطة، ضغطتين تمام؟ باخد الحجم اللي بديها مع
+
+331
+00:24:00,870 --> 00:24:05,750
+extra تمام؟و لما اجى افضى فى المحلول بتغضى تواحدة
+
+332
+00:24:05,750 --> 00:24:11,030
+بحيث ان انا افضى فقط المحلول الحجم اللى بديه و يضل
+
+333
+00:24:11,030 --> 00:24:14,330
+ال extra اللى موجود فى التب اتخلص منه طب انا ليش
+
+334
+00:24:14,330 --> 00:24:20,430
+يعني بلجأ لهذه الطريقة بحكيلي لإنه فى سوائل عبارة
+
+335
+00:24:20,430 --> 00:24:25,250
+عن لازجة viscous طبعا هاد اش يعني viscous لازجة
+
+336
+00:24:25,250 --> 00:24:29,130
+يعني بصعوبة هتطلع جوا التب المحليل بصعوبة هتطلع
+
+337
+00:24:29,130 --> 00:24:35,520
+جوا التب طبعا طيبفبالتالي لما اجي انا اكون ماخدة
+
+338
+00:24:35,520 --> 00:24:39,200
+ال volume وزيادة شوية هكون ضامنة ان انا اخدت ال
+
+339
+00:24:39,200 --> 00:24:42,060
+volume ..يعني ضامنة ان انا هنزل ال volume على
+
+340
+00:24:42,060 --> 00:24:47,480
+الأقل اللي بديها، تمام؟ لكن مثلا لو انا ده استخدمت
+
+341
+00:24:47,480 --> 00:24:52,430
+مع الهادي المحلي للviscousاستخدمت نظامة ضغطة لوحدة
+
+342
+00:24:52,430 --> 00:24:57,050
+ما هي بتطلع جوا التب بطء يعني انا ممكن افوت و اطلع
+
+343
+00:24:57,050 --> 00:25:01,590
+و اخد ال باقي الحجم اللي بدياه بس لسه السائل من
+
+344
+00:25:01,590 --> 00:25:08,200
+كتر لزوجته ماعباش جوا التب بشكلكامل فبالتالي ممكن
+
+345
+00:25:08,200 --> 00:25:12,800
+اخد volume اقل من اللي انا بديها فهنا ممكن يكون في
+
+346
+00:25:12,800 --> 00:25:17,120
+خطأ عشان هيك بحكيلي انه استخدمي مع السوائل اللازجة
+
+347
+00:25:17,120 --> 00:25:21,200
+انه طريقة انك انت تاخدي بزيادة و لما تفض تفضي
+
+348
+00:25:21,200 --> 00:25:27,230
+الحجم اللي بدكياتمام هذا بالنسبة لمين ل .. ال ..
+
+349
+00:25:27,230 --> 00:25:31,150
+ال .. السواقل اللازجة بستخدم معاها ال reverse
+
+350
+00:25:31,150 --> 00:25:34,330
+technique يعني نظام الضغطين عشان أخد extra volume
+
+351
+00:25:34,330 --> 00:25:37,590
+أيضا بيحكيلي فيه محاليل volatile إيش يعني
+
+352
+00:25:37,590 --> 00:25:42,030
+volatile؟ يعني متطايرة يعني مجرد مثلا ما أسحبها في
+
+353
+00:25:42,030 --> 00:25:46,350
+ال pipe و أنقلها لل .. للوعائل عشان أفضيها ممكن
+
+354
+00:25:46,350 --> 00:25:50,810
+تكون اتبخرت فهنا بيصير في عندي فاقد في الحجم
+
+355
+00:25:50,810 --> 00:25:58,290
+فبالتالي أنا بكون نقلت حجمأقل من اللازم فهنا باع
+
+356
+00:25:58,290 --> 00:26:05,490
+هذه النوع من السوائل ايضا ممكن انه استخدم نظام
+
+357
+00:26:05,490 --> 00:26:09,230
+الضغطين بحيث ان انا اخد حجم بزيادة و لما افضل افضل
+
+358
+00:26:09,230 --> 00:26:14,030
+exact volume عشان اذا مهما يكون الطاير من هذا ال
+
+359
+00:26:14,030 --> 00:26:18,050
+solution اكون على الأقل مازلة محتفظة بال volume
+
+360
+00:26:18,050 --> 00:26:21,890
+اللي انا بديهاتمام؟ أيضا في بعض المحاليل اللي
+
+361
+00:26:21,890 --> 00:26:24,510
+بنسميها foaming solution ايش يعني foaming
+
+362
+00:26:24,510 --> 00:26:28,950
+solution؟ يعني محاليل بتعمل رغبة تمام؟ فيها مادة
+
+363
+00:26:28,950 --> 00:26:33,190
+صابون او يعني مادة ..الها ملمة الصابونة هذه
+
+364
+00:26:33,190 --> 00:26:37,690
+المحاليل اللي فيها مواد ذات ملمة الصابونة تمام؟
+
+365
+00:26:37,690 --> 00:26:41,120
+ممكن تعملي رغبةو بالتالي لما أجي أصحب أصحب من
+
+366
+00:26:41,120 --> 00:26:45,220
+الرغبة high فبالتالي أكون نقلت volume أقل من
+
+367
+00:26:45,220 --> 00:26:49,520
+اللازم لإن أنا يعني جزء من الرغبة احتلت ال tub
+
+368
+00:26:49,520 --> 00:26:53,300
+تمام؟ و أفكر حالي إن أنا أخدت الحجم اللي بدياه و
+
+369
+00:26:53,300 --> 00:26:59,700
+فعليا أنا بكون ماخدة حجم أقل من ال ..المفروض
+
+370
+00:26:59,700 --> 00:27:03,300
+فبالتالي بستخدم برضه نظام ال larger volume of
+
+371
+00:27:03,300 --> 00:27:06,120
+solution يعني ان انا بدي اخد ال extra volume يعني
+
+372
+00:27:06,120 --> 00:27:09,700
+بدي استخدم ال reverse technique يعني نظام ضغطتين و
+
+373
+00:27:09,700 --> 00:27:17,300
+.. و بعد هيك شسمه بروح بفضي بضغطة واحدةتمام؟ عشان
+
+374
+00:27:17,300 --> 00:27:21,780
+لو كان في رغبة في المحلول أكون ضامنة أنها ما تأثر
+
+375
+00:27:21,780 --> 00:27:25,360
+على الحجم اللي أنا بدي أنقله تمام؟ و أيضا عشان لو
+
+376
+00:27:25,360 --> 00:27:29,180
+أنا بدي أنقل حجوم صغيرة جدا جدا يعني أنا خايفة ..
+
+377
+00:27:29,180 --> 00:27:32,260
+يعني مش متأكدة أخدت ال volume ولا لأ لأنه في حجوم
+
+378
+00:27:32,260 --> 00:27:35,600
+يعني زي مثلا حجم one microliterيعني انت حتى ما
+
+379
+00:27:35,600 --> 00:27:39,040
+بتشوفيه على التب يعني صعب تشوفيه على التب انه انت
+
+380
+00:27:39,040 --> 00:27:42,720
+أخدتي او ما أخدتي فالأفضل ان انا ااخد حجم بزيادة و
+
+381
+00:27:42,720 --> 00:27:45,560
+لما افضي داخل ال solution او المقلوعه اللي انا بدي
+
+382
+00:27:45,560 --> 00:27:50,640
+اشتغل فيه بفضي بالحجم اللي بديها اذا .. اذا لما
+
+383
+00:27:50,640 --> 00:27:56,550
+انا اسحب الحجمبضغطة و افضيه بدغطتين معناها انا بدي
+
+384
+00:27:56,550 --> 00:28:01,050
+اخدت exact volume و افضيته بدغطتين عشان اضمن ان
+
+385
+00:28:01,050 --> 00:28:05,270
+انا افضيته كويس تمام هذه طريقة forward bibuting
+
+386
+00:28:05,270 --> 00:28:10,510
+لكن لما انا اسحب الحجم بدغطتين و افضيه بدغطة واحدة
+
+387
+00:28:10,510 --> 00:28:14,590
+معناها انا استخدمت طريقة ال reverse bibuting و هذا
+
+388
+00:28:14,590 --> 00:28:20,490
+طبعا بيختلفهلأ ال .. ال .. اذا هذه ال bybit شايفين
+
+389
+00:28:20,490 --> 00:28:24,570
+هي البلانجر بالكبس عليه في عندى ضغطة واحدة او
+
+390
+00:28:24,570 --> 00:28:28,690
+ضغطتان depress the blanger until you feel the
+
+391
+00:28:28,690 --> 00:28:32,090
+initial resistance مجرد ما انت تضغطي طبعا ان شاء
+
+392
+00:28:32,090 --> 00:28:35,670
+الله راح نرجع على المعامل و امسكهم ال bybit كل بنت
+
+393
+00:28:35,670 --> 00:28:39,770
+راح يكونيلها bybitو تشوف و تجرب كيف تشتغل عليها
+
+394
+00:28:39,770 --> 00:28:43,130
+كيف تضغط .. كيف لما بتضغط بتقابل ال resistant
+
+395
+00:28:43,130 --> 00:28:46,750
+الأولى اللي هي الإعاقة الأولى يعني إشي بيعيط مصاري
+
+396
+00:28:46,750 --> 00:28:50,590
+ال blanket إشي بيوقفه هذه بتكون الدغطة الأولى و
+
+397
+00:28:50,590 --> 00:28:55,390
+بقى لو .. لو كبست بقوة أكبر بتكون ساعتها أخدت ال
+
+398
+00:28:55,390 --> 00:28:59,730
+.. ال .. ال vibe أخدت الدغطتين تمام فيعني بصير ده
+
+399
+00:28:59,730 --> 00:29:05,530
+تسحب extra volumeطيب فبالتالي لما احنا بدنا نفوت
+
+400
+00:29:05,530 --> 00:29:09,090
+في ال .. ال .. ال vibe طبعا لما اجي افوتها بفوتها
+
+401
+00:29:09,090 --> 00:29:13,190
+بحيث انها تكون مغموقة جزء منها مغمور او جزء من
+
+402
+00:29:13,190 --> 00:29:18,330
+التب مغمور داخل ال solution تمام؟ مابروح اخلي
+
+403
+00:29:18,330 --> 00:29:23,290
+الفتحة التب او هنا يعني الفوهة التب ملامسة للسطح
+
+404
+00:29:23,290 --> 00:29:26,450
+لإنه انا ممكن اسحب سائل و هوا مع بعض مدام ها على
+
+405
+00:29:26,450 --> 00:29:31,690
+السطحو أيضا كمان ما بخليها تلامس البطم لل solution
+
+406
+00:29:31,690 --> 00:29:36,630
+ليش؟ لإن هذه الفوهة أو فتحة التب ممكن يغلقها اللي
+
+407
+00:29:36,630 --> 00:29:43,810
+هي قاع ال container او ال tube فبالتالي انه انا
+
+408
+00:29:43,810 --> 00:29:48,550
+بكون هي كعملت انسداد للتب و مش راح اسحب ال volume
+
+409
+00:29:48,550 --> 00:29:52,730
+اللي بدي يعني تمام؟ اذا بضغطال .. الضغطة أولة أو
+
+410
+00:29:52,730 --> 00:29:57,510
+ضغطة تانية حسب نوع ال .. ال .. ال bybiting لو أنا
+
+411
+00:29:57,510 --> 00:30:01,890
+بستخدم forward bybiting هضغط ضغطة واحدة تمام بفوت
+
+412
+00:30:01,890 --> 00:30:04,830
+ال tip بحيث أنه يكون جزء منها مغمور داخل ال
+
+413
+00:30:04,830 --> 00:30:08,050
+solution مش على السطح ولا على .. في القاع م ..
+
+414
+00:30:08,050 --> 00:30:12,630
+ملامسة للقاع و ببدأ بعد هيك إيش أعمل أرفع إيدي
+
+415
+00:30:12,630 --> 00:30:18,350
+بالتدريج عن البلانجر بالتدريج تمام مهم جدا أن أنا
+
+416
+00:30:18,350 --> 00:30:22,860
+أرفع إيدي بالتدريج عن البلانجرمارفعش هيك فجأة
+
+417
+00:30:22,860 --> 00:30:28,380
+واحدة ليش؟ لإن لو رفعت فجأة راح يندفع ال solution
+
+418
+00:30:28,380 --> 00:30:33,520
+بقوة داخل ال .. ال .. التب فهذا هيسببلي أخطاء ممكن
+
+419
+00:30:33,520 --> 00:30:37,340
+ما أخد ال volume اللي بديه ممكن ال .. ال .. السائل
+
+420
+00:30:37,340 --> 00:30:40,940
+يندفع ويفوت داخل ال pipe ذات نفسها تمام؟ يعني يطلع
+
+421
+00:30:40,940 --> 00:30:44,560
+من التب ويفوت داخل ال pipe ويقدي إلى الصدق ل ..
+
+422
+00:30:44,560 --> 00:30:49,380
+لإلها أو إلى التلف تمام؟ وممكن أكون يعني يسحب هوا
+
+423
+00:30:49,750 --> 00:30:52,750
+معاها يعني يكون فيه فقاعات فبالتالي يكون فيه error
+
+424
+00:30:52,750 --> 00:30:56,830
+في ال volume اللي نقلته فبالتالي يفضل انه انا لما
+
+425
+00:30:56,830 --> 00:31:00,910
+اجي ارفع البلانجر ارفعه بالتدريج عشان المحلول يفوت
+
+426
+00:31:00,910 --> 00:31:05,710
+بالتدريج او ببطء داخل ال pipet ويفوت بشكل دقيق و
+
+427
+00:31:05,710 --> 00:31:11,220
+اكون انا عم باخد الحجم الدقيق تمام؟بعد هيك بروح
+
+428
+00:31:11,220 --> 00:31:15,120
+على ال container التاني و ب .. أضغط على البلانجر
+
+429
+00:31:15,120 --> 00:31:19,720
+مرتين يعني على ال initials ال resistant و بفضيه و
+
+430
+00:31:19,720 --> 00:31:22,320
+ممكن أضغط على ال second resistant عشان أتأكد أن
+
+431
+00:31:22,320 --> 00:31:26,040
+أنا فضيت كل ال volume و بعد هيك بتخلص من ال tip
+
+432
+00:31:26,040 --> 00:31:29,080
+كيف بتخلص من ال tip؟ حكينا أنه في هنا على الطرف
+
+433
+00:31:29,080 --> 00:31:33,720
+حاجة اسمها ejector لل tipهنا مجرد ما انا اضغط على
+
+434
+00:31:33,720 --> 00:31:37,500
+هذا ال ejector لل tip ال tip بتكون خلاص فانفصلت عن
+
+435
+00:31:37,500 --> 00:31:43,100
+ال pipet و انا بتخلص منها فلما بدي اتخلص من ال tip
+
+436
+00:31:43,100 --> 00:31:47,520
+بوجه ال pipet هذه باتجاه السلة تمام و بضغط على ال
+
+437
+00:31:47,520 --> 00:31:53,860
+ejector فلحالها ال pipet بتروح على الفضلات
+
+438
+00:31:54,650 --> 00:31:59,550
+ع الوسط طبعا هذا بالنسبة ل .. ال .. ال forward
+
+439
+00:31:59,550 --> 00:32:05,230
+بالنسبة ل reverse طبعا يعني تقريبا نفس التعليمات
+
+440
+00:32:05,230 --> 00:32:08,270
+ولكن انه انا لما بدي افوت ضغطة بدي اكون ضغطة
+
+441
+00:32:08,270 --> 00:32:13,250
+ضغطتين و بدي افوت ب .. و اكون جزء من الطب مغمور
+
+442
+00:32:13,250 --> 00:32:17,510
+داخل ال solution طبعا بفوت بشكل عمودي يا بنات لو
+
+443
+00:32:17,510 --> 00:32:20,710
+انا بدي افوت بشكل مائل بيأثر على الحجم اللي انا
+
+444
+00:32:20,710 --> 00:32:24,510
+مخدعةبفوت بشكل عمودي داخل ال solution جزء من ال
+
+445
+00:32:24,510 --> 00:32:29,230
+tip مغمور داخل ال solution و برفع ايدي بالتدريج عن
+
+446
+00:32:29,230 --> 00:32:32,670
+البلنجر بسحبلي ال volume طبعا هنا في ال reverse
+
+447
+00:32:32,670 --> 00:32:37,190
+انا ضغطت ضغطتين فهيسحبلي extra volume تمام؟بعدين
+
+448
+00:32:37,190 --> 00:32:40,570
+بروح على ال container اللي انا بدي افض فيه و بضغط
+
+449
+00:32:40,570 --> 00:32:44,230
+ضغطة واحدة علشان تنزلي ال solution اللي انا بديها
+
+450
+00:32:44,230 --> 00:32:48,210
+فقط الحجم اللي بديها فقط و ال extra هو و ال tab
+
+451
+00:32:48,210 --> 00:32:50,730
+بتخلص منه في ال west من خلال ان انا اضغط على ال
+
+452
+00:32:50,730 --> 00:32:54,590
+ejector و تنفصل ال tab عن ال pipet و تروح في حال
+
+453
+00:32:54,590 --> 00:33:00,370
+سبيلهمتمام؟ أول حاجة مع .. يعني ملاحظات لاستخدام
+
+454
+00:33:00,370 --> 00:33:04,650
+ال bybit, never point a bybit up ممنوع أنك انت
+
+455
+00:33:04,650 --> 00:33:07,970
+تستخدمي ال bybit تقليبيها بهذا الشكل بحيث انها
+
+456
+00:33:07,970 --> 00:33:13,600
+تكون ال tip صارت فوق و ال bybit تحتطبعا و انا
+
+457
+00:33:13,600 --> 00:33:16,300
+دايما لما بستخدم ال bible بتكون بهذا الاتجاه بحيث
+
+458
+00:33:16,300 --> 00:33:19,620
+أنه التب لا تحت لإنه لو أنا بدي أجلبها ال volume
+
+459
+00:33:19,620 --> 00:33:22,680
+اللي موجود في التب راح يفوت داخل ال bible طبعا هذه
+
+460
+00:33:22,680 --> 00:33:28,420
+عبارة عن أنا بيب طبعا و في كمان نظام ال .. ال ..
+
+461
+00:33:28,420 --> 00:33:33,460
+الذنبركاتفبالتالي ممكن تصدق داخل ال .. يعني يصير
+
+462
+00:33:33,460 --> 00:33:37,300
+صدق او يصير فيه تلف و ممكن في محاليل بترسب بتعمل
+
+463
+00:33:37,300 --> 00:33:41,440
+رواس بتعمل انسداد فهذا بيأثر على دقة ال pipet فهو
+
+464
+00:33:41,440 --> 00:33:45,340
+يفضل ان انا ممنوع اوجهها الى اعلى بحيث ان ال
+
+465
+00:33:45,340 --> 00:33:48,580
+liquid يفوت داخل ال pipet و يعملها destroying
+
+466
+00:33:48,580 --> 00:33:53,060
+تمام؟طيب when with a drawing liquid with with a
+
+467
+00:33:53,060 --> 00:33:56,540
+pipe it always release the plunger slowly طبعا
+
+468
+00:33:56,540 --> 00:34:00,720
+لحظة لاحظته انتوا حتى بنبرت صوتي ركزتي كتير منيح و
+
+469
+00:34:00,720 --> 00:34:04,020
+انا بحكيلكم لما ترفع ايدك عن ال plunger ارفعيه
+
+470
+00:34:04,020 --> 00:34:09,040
+slowly شويه شويه بالتدريج بهدوء مابرفع ايدي فجأة
+
+471
+00:34:09,040 --> 00:34:13,800
+ليش لإن هنا هي بحكيلك انه ال .. ال .. ال ..الشغل
+
+472
+00:34:13,800 --> 00:34:19,280
+رافعت ايدك بشكل تدريجي او بشكل بطيء عن البلانجر
+
+473
+00:34:19,280 --> 00:34:24,080
+هيمنع من انه ال liquid يصير له rushing into انه
+
+474
+00:34:24,080 --> 00:34:28,160
+يندفع داخل ال pipe و بالتالي ممكن يسببلها انسداد
+
+475
+00:34:28,160 --> 00:34:35,100
+او صدق او تلف فبالتاليمهم جدا طبعا انه ارفع ايدي
+
+476
+00:34:35,100 --> 00:34:38,020
+بالتدريج this is especially important with large
+
+477
+00:34:38,020 --> 00:34:41,860
+volumes يعني و هذا الاشي بنتبهله كتير منيح و انا
+
+478
+00:34:41,860 --> 00:34:46,740
+بصحب حجوم كبيرة حجوم كبيرة معناها السائل هيندفع
+
+479
+00:34:46,740 --> 00:34:50,720
+بشكل قوي هو كميته كبيرة فهيفوت داخل ال bipe هيصل ل
+
+480
+00:34:50,720 --> 00:34:55,240
+ال bipe من جوا و يتلفها be sure you use the proper
+
+481
+00:34:55,240 --> 00:35:02,780
+size tip for each bipe حكينا انه لكلبيبت طبعا لكل
+
+482
+00:35:02,780 --> 00:35:08,040
+بيبت في عندى تب خاصة فيها تبعا لحجمها أو تبعا ل ال
+
+483
+00:35:08,040 --> 00:35:12,380
+range تبعها فانا كل بيبت بستخدملها التب الخاصة
+
+484
+00:35:12,380 --> 00:35:15,340
+فيها ما بنفع مثلا مع البيبتات الصغيرة أستخدم تب
+
+485
+00:35:15,340 --> 00:35:19,040
+كبيرة أصلا مش راح تركب عليها وما بنفع مع البيبتات
+
+486
+00:35:19,040 --> 00:35:21,980
+الكبيرة أستخدم تب صغيرة مش راح تكفي لل volume اللي
+
+487
+00:35:21,980 --> 00:35:26,520
+أنا بدني أنقله طبعا فإذا كل بيبت لها تب خاصة فيها
+
+488
+00:35:26,520 --> 00:35:30,740
+بستخدمهاalways use a new tip for each different
+
+489
+00:35:30,740 --> 00:35:35,960
+liquid طبعا كل حجم بدي أنقله بستخدمله a new tip
+
+490
+00:35:35,960 --> 00:35:40,020
+طبعا ال tip جديدة و حكينا انه هاي ال tip أشمالها
+
+491
+00:35:40,020 --> 00:35:44,460
+disposable طبعا فهيمر معانا ان شاء الله مصطلح
+
+492
+00:35:44,460 --> 00:35:48,360
+disposable و أذكركم فيه يعني تستخدم لمرة واحدة فقط
+
+493
+00:35:48,640 --> 00:35:52,580
+طيب use the correct pipet for the volume to be
+
+494
+00:35:52,580 --> 00:35:56,260
+dispended يعني انا بدي استخدم ال pipet اختار ال
+
+495
+00:35:56,260 --> 00:36:02,180
+pipet اللي ال range تبعها تمام مناسب ل .. ال .. ال
+
+496
+00:36:02,180 --> 00:36:04,980
+.. ال pipet ل الحجم اللي بدي أنقله هى مثلا هنا
+
+497
+00:36:04,980 --> 00:36:09,420
+بيحكيلك never use the متين ل الف microliter pipet
+
+498
+00:36:09,420 --> 00:36:12,720
+to dispense متين تمام هى هنا مثلا هى ال range من
+
+499
+00:36:12,720 --> 00:36:18,210
+متين ل الف مابستخدمها عشان انقل حجم متينلأن حكينا
+
+500
+00:36:18,210 --> 00:36:20,870
+إن هي مش هتكون دقيقة على lower limit لكن ممكن تكون
+
+501
+00:36:20,870 --> 00:36:23,630
+دقيقة على upper limit أو بتكون أدقهي على upper
+
+502
+00:36:23,630 --> 00:36:28,750
+limit تمام؟ و لو أنا بدي أخد حجوم أقل من 200 أو
+
+503
+00:36:28,750 --> 00:36:33,790
+أكتر من ألف هذا إيش ممكن يعملي سببلي damage للأداة
+
+504
+00:36:33,790 --> 00:36:39,710
+هذه فبالتالي لازم أكون حذرة و حريصة في استخدام ال
+
+505
+00:36:39,710 --> 00:36:44,850
+bybit without the bybitwithdraw the pipet from the
+
+506
+00:36:44,850 --> 00:36:48,290
+receiving vessels carefully sliding the tip along
+
+507
+00:36:48,290 --> 00:36:51,890
+the wall هلأ مجرد ما انا ارفع ال pipet بعد ما اخد
+
+508
+00:36:51,890 --> 00:36:55,610
+الحجم اللي بديا لازم اعمل حاجة اسمها sliding يعني
+
+509
+00:36:55,610 --> 00:37:00,750
+انا امرر ال tip هادي امسحها على فتحة ال container
+
+510
+00:37:00,750 --> 00:37:05,690
+يعني مثلابهذا الشكل يعني ارفع وانزل ال buy bet على
+
+511
+00:37:05,690 --> 00:37:11,130
+اللي فوهت او فتحت ال container تبع المحلول بحيث
+
+512
+00:37:11,130 --> 00:37:15,910
+انه اي اشي عالق على جدار ال tip من برا اتخلص منه
+
+513
+00:37:15,910 --> 00:37:19,330
+انا بعنيني فقط ال volume اللي انا نقلته في الداخل
+
+514
+00:37:19,330 --> 00:37:23,670
+طبعا فبالتالي عشان ما اخد excess volume remove the
+
+515
+00:37:23,670 --> 00:37:30,770
+tip from the sample liquidتمام؟ و no liquid should
+
+516
+00:37:30,770 --> 00:37:35,130
+remain on the outside of the tip طبعا يعني لما ..
+
+517
+00:37:35,130 --> 00:37:40,130
+مجرد ما انا اطلع من ال .. ال .. يعني اخد الحجم
+
+518
+00:37:40,130 --> 00:37:43,410
+اللي بديها و اطلع من ال container مش لازم يضل
+
+519
+00:37:43,410 --> 00:37:47,910
+بقايا من ال solution على ال tip فيفضل ان انا امسح
+
+520
+00:37:47,910 --> 00:37:52,620
+باستخدام ال wipe تمام؟او ال .. ال جوز او اي قطعة
+
+521
+00:37:52,620 --> 00:37:58,780
+.. قطعة ت .. clinics او قطن او شاش باجفف ال .. ال
+
+522
+00:37:58,780 --> 00:38:02,780
+.. ال bybit بمسحها بمررها عليها بحيث ان انا اجفف
+
+523
+00:38:02,780 --> 00:38:07,300
+اي بقايا عالقة عليها ولكن يف .. بكون حريصة انه
+
+524
+00:38:07,300 --> 00:38:12,600
+هادي ال .. ال .. ال vibe تمامما تلامس فوهة التب
+
+525
+00:38:12,600 --> 00:38:17,540
+ليش لإنها لو لامست فوهة التب هذا absorbent المحارم
+
+526
+00:38:17,540 --> 00:38:21,000
+و ال clinics و الاشي بينطس السائل فممكن تمطس
+
+527
+00:38:21,000 --> 00:38:25,820
+السائل اللي موجود داخل التب و بالتالي بينقص ال
+
+528
+00:38:25,820 --> 00:38:28,560
+volume اللي أخدته و بيكون أنا هيك عم باخد volumes
+
+529
+00:38:28,560 --> 00:38:32,260
+مش accurate و مش precise تمام مهم جدا لها هاي
+
+530
+00:38:32,260 --> 00:38:37,440
+الشغل اذا بعد هى مجرد ما إنه أسحب ال .. ال .. ال
+
+531
+00:38:37,440 --> 00:38:37,860
+.. ال .. ال .. ال .. ال .. ال .. ال .. ال .. ال ..
+
+532
+00:38:37,860 --> 00:38:38,800
+ال .. ال .. ال .. ال .. ال .. ال .. ال .. ال .. ال
+
+533
+00:38:38,800 --> 00:38:39,540
+.. ال .. ال .. ال .. ال .. ال .. ال .. ال .. ال ..
+
+534
+00:38:39,540 --> 00:38:39,680
+ال .. ال .. ال .. ال .. ال .. ال .. ال .. ال .. ال
+
+535
+00:38:39,680 --> 00:38:39,680
+.. ال .. ال .. ال .. ال .. ال .. ال .. ال .. ال ..
+
+536
+00:38:39,680 --> 00:38:43,650
+ال .. المش لازم no liquid should remain on the
+
+537
+00:38:43,650 --> 00:38:46,950
+outside مش لازم ال liquid يضل على خارج ال tip
+
+538
+00:38:46,950 --> 00:38:51,470
+فبالتالي بمسح قطارات اللي .. اللي موجودة على ال
+
+539
+00:38:51,470 --> 00:38:56,190
+outside of the tip بستخدام tissue, paper, جوز,
+
+540
+00:38:56,730 --> 00:39:01,130
+wipes أي إش .. أي نوع محارب أو clinics بحيث انه ما
+
+541
+00:39:01,130 --> 00:39:04,790
+ألامس never touch the tip orifice مافتحق ماألامسش
+
+542
+00:39:04,910 --> 00:39:08,290
+ال tip orifice يعني فتحة ال .. ال tip ال opening
+
+543
+00:39:08,290 --> 00:39:12,410
+تبع ال tip طبعا ماقلامسه بحيث انه ماينطست السائل و
+
+544
+00:39:12,410 --> 00:39:16,770
+بهك انا اكون عم باخد volume اقل من اللازم و اكون
+
+545
+00:39:16,770 --> 00:39:19,870
+مش .. مش accurate و مش precise في عملية ال volume
+
+546
+00:39:19,870 --> 00:39:25,090
+transfer طبعا طيب pre-rinsing ايش يعني كلمة pre
+
+547
+00:39:25,090 --> 00:39:31,610
+-rinsing يفضل انه انا .. انا لما بدي انقل حجوم ان
+
+548
+00:39:31,610 --> 00:39:39,390
+انا ابلليعني مثلا هنا يفضل إن أنا أبلل ال .. ال ..
+
+549
+00:39:39,390 --> 00:39:44,290
+التب من جوا في .. في .. في السائل اللي بدي أنقله،
+
+550
+00:39:44,290 --> 00:39:47,510
+هاي معناه pre runs، طبعا؟ يعني أول حاجة بصحب أول
+
+551
+00:39:47,510 --> 00:39:52,330
+volume و بتخلص منه بكبه، هيك أنا بضمن إنه جزء من
+
+552
+00:39:52,330 --> 00:39:56,570
+السائل لمس ال .. ال .. التب من الداخل فبالتالي
+
+553
+00:39:56,570 --> 00:40:02,570
+صارت التب إشمالها مبللة، طيب فلمّا أجي أنقل volume
+
+554
+00:40:03,230 --> 00:40:06,390
+و أاخد ال volume و أنقله هيك بكون أنا عم باخد
+
+555
+00:40:06,390 --> 00:40:10,490
+volume بشكل أدق ليش؟ لأنه لو .. لو أنا ماعملت ال
+
+556
+00:40:10,490 --> 00:40:14,490
+pre-rinsing هذه طبعا لو ماعملت pre-rinsing أخدت
+
+557
+00:40:14,490 --> 00:40:18,350
+volume و روحت فضيته في جزء من السائل اللي أنا
+
+558
+00:40:18,350 --> 00:40:24,370
+أخدته بيضل ملتصق بجدران التب مبلل لإلها وهذا جزء
+
+559
+00:40:24,370 --> 00:40:29,190
+يعني في نسبة فقد لل volume في نسبة errorطبعا لكن
+
+560
+00:40:29,190 --> 00:40:34,490
+لما انا استخدم tip already مبللة يعني tip انا سحبت
+
+561
+00:40:34,490 --> 00:40:36,970
+اول volume فيها و كبيته و بعدين سحبت ال volume
+
+562
+00:40:36,970 --> 00:40:40,290
+اللى بدي استخدمه ال volume الفعلي معناه انا بللت
+
+563
+00:40:40,290 --> 00:40:45,850
+ال tip من الداخل فبالتالي فبطل في عندي نسبة فقد من
+
+564
+00:40:45,850 --> 00:40:49,890
+الحجم و بطل في عندي نسبة ال error هذه معناه كلمة
+
+565
+00:40:49,890 --> 00:40:53,670
+pre-rinsing minimize اشمالها ال variances
+
+566
+00:40:53,670 --> 00:40:59,560
+الاختلافات في ال .. ال ..الحجوم طبعا when by
+
+567
+00:40:59,560 --> 00:41:03,080
+beating some solutions زي ال serum اللي هي العينات
+
+568
+00:41:03,080 --> 00:41:08,260
+يعني الشخ .. المرضى ال serum البلازما ال protein
+
+569
+00:41:08,260 --> 00:41:11,300
+containing solution المحليل اللي بتحتوي على
+
+570
+00:41:11,300 --> 00:41:14,420
+البروتينات ال organic solvents المضيبات العضوية
+
+571
+00:41:14,420 --> 00:41:19,700
+هذه طبعا في جزء منها بيكون حكينا انه هيصير في فيلم
+
+572
+00:41:19,700 --> 00:41:24,500
+هيفي جزء منها هيبطن هيلامس ال inside of the tipو
+
+573
+00:41:24,500 --> 00:41:29,200
+هذا حكينا اشماله error فبالتالي لما انا اعمل عملية
+
+574
+00:41:29,200 --> 00:41:33,540
+pre-rinsing فبالتالي انا بقلل ال error هذه من خلال
+
+575
+00:41:33,540 --> 00:41:39,120
+ان انا خلاص استخدمت already جزء بيبة مبللة جزء ..
+
+576
+00:41:39,120 --> 00:41:43,040
+بيبة عملنا لها pre-rinsing فبالتالي مش هيكون في
+
+577
+00:41:43,040 --> 00:41:47,400
+عندي فقد في ال volume و مش هيكون في عندي نسبة
+
+578
+00:41:47,400 --> 00:41:51,780
+errorطيب when by beating concentrated acid or
+
+579
+00:41:51,780 --> 00:41:56,100
+highly corrosive solution such as halite salts
+
+580
+00:41:56,100 --> 00:42:01,540
+it's recommended to that by bit be disassembled
+
+581
+00:42:01,540 --> 00:42:07,680
+after used to inspect the shaft و المكونات تبعتها،
+
+582
+00:42:07,680 --> 00:42:11,800
+هلأ بيحكيلك أنه لما بنستخدم بعض المحاليل اللي هي
+
+583
+00:42:11,800 --> 00:42:18,100
+corrosive تمامحارقة او يعني مؤدية وبيتطير منها
+
+584
+00:42:18,100 --> 00:42:24,160
+ابخرة او يعني فيه بيتصعد منها قترانات او ابخرة او
+
+585
+00:42:24,160 --> 00:42:28,960
+رذاذ زي ال .. ال .. الهاليد سالتز هاي فيفضل ان انا
+
+586
+00:42:28,960 --> 00:42:32,620
+افك البعيبة و انظفها من الداخل و ارجع اركبها مرتان
+
+587
+00:42:32,620 --> 00:42:38,940
+علشان اتأكد انها هي يعني ما رح تتعطل ما رح تخرب ال
+
+588
+00:42:38,940 --> 00:42:44,050
+air bubbleطبعا يعني ان في عندي تقطع في السائل اللي
+
+589
+00:42:44,050 --> 00:42:47,170
+انا عم باخده في هنا فيه قطارات في فيه .. فيه هوا
+
+590
+00:42:47,170 --> 00:42:52,670
+تمام؟طب هذا ال .. ال .. الهواء إيش سببه؟ سببه أول
+
+591
+00:42:52,670 --> 00:42:55,970
+حاجة إما إن أنا رفعت إيدي عن ال plunger بشكل سريع
+
+592
+00:42:55,970 --> 00:43:00,010
+أو كانت ال tip مش مغمورة كويس في ال solution سحبت
+
+593
+00:43:00,010 --> 00:43:03,550
+من عن السطح فبالتالي بما إن سحبت عن السطح معناها
+
+594
+00:43:03,550 --> 00:43:10,810
+صار في عرض لإنه يدخل air bubble تمام ممكن يكون إنه
+
+595
+00:43:11,460 --> 00:43:19,940
+سببها ال .. ال .. يعني السائل نفسه يكون يعني فومي
+
+596
+00:43:19,940 --> 00:43:26,480
+بي .. بيكون رغوة فبالتالي انا ما اتبعت التعليمات ل
+
+597
+00:43:26,480 --> 00:43:31,360
+.. ل .. ل .. ال by betting من فومي reagent
+
+598
+00:43:31,360 --> 00:43:36,240
+فبالتالي ممكن تظهر عندى air bubble طبعا ال .. ال
+
+599
+00:43:36,240 --> 00:43:40,190
+.. ال air bubble إذا ظهرت طبعاطبعا أول حاجة إذا
+
+600
+00:43:40,190 --> 00:43:43,910
+ظهرت في .. في ال by bit بفضل solution و برجع باخد
+
+601
+00:43:43,910 --> 00:43:47,490
+مرة تانية، ضالت موجودة معناها بيده أتخلص من التب و
+
+602
+00:43:47,490 --> 00:43:51,090
+أركب تب جديدة و أاخد volume مع ال .. التزام
+
+603
+00:43:51,090 --> 00:43:56,410
+بالتعليمات حتى أتجنب من ظهور air bubble، ليش؟ لإن
+
+604
+00:43:56,410 --> 00:43:59,150
+ال air bubble طبعا هذه محتلة جزء من الحجم اللي
+
+605
+00:43:59,150 --> 00:44:03,580
+موجود يعني هذا المفروض كله يكون سائلطبعا فوجود air
+
+606
+00:44:03,580 --> 00:44:06,860
+bubble وجود تقطع في السائل يعني فيه هوى فبالتالي
+
+607
+00:44:06,860 --> 00:44:09,900
+الهوى محتل حجم اذا انا مش ماخدة الحجم المناسب
+
+608
+00:44:09,900 --> 00:44:14,360
+ماخدة اقل منه اذا انا مش accurate ومش precise طبعا
+
+609
+00:44:14,360 --> 00:44:19,420
+هذا بالنسبة ل ال bybit طيب خلينا بس قبل ما نشوف ال
+
+610
+00:44:19,420 --> 00:44:23,580
+calibration ل ال bybit نتابع مع بعض نشوف هذا
+
+611
+00:44:23,580 --> 00:44:30,040
+الفيديو بيعلمناكيف انا بدي استخدم ال .. ال by bet
+
+612
+00:44:30,040 --> 00:44:34,000
+و هنرجع نحكي نفس التعليمات او نفس المعلومات اللي
+
+613
+00:44:34,000 --> 00:44:37,560
+انا ذكرتهم باستخدام ال by bet هاي زي ما انتوا
+
+614
+00:44:37,560 --> 00:44:42,340
+شايفين هنا خليني بس ارجع شوية هنا تمام هاي ال by
+
+615
+00:44:42,340 --> 00:44:48,640
+bet نفس المكونات زي ما انتوا شايفين هنا نفس
+
+616
+00:44:48,640 --> 00:44:52,400
+المكونات لحظة خليني ارجع
+
+617
+00:44:55,930 --> 00:44:59,770
+خلص من هاي، هاي ال pipe هاي البلانجر تمام و الرد
+
+618
+00:44:59,770 --> 00:45:03,090
+هذا اللي بيلتف يمين و يسار تمام عكس حقارب الساعة و
+
+619
+00:45:03,090 --> 00:45:06,470
+باتجاه حقارب الساعة باتجاهي او بعيد عني حسب ما انا
+
+620
+00:45:06,470 --> 00:45:10,930
+هعلمكم ان شاء الله و هاي ال .. ال .. ال screen
+
+621
+00:45:10,930 --> 00:45:16,350
+تمام هذا ال .. ال .. ال ejector طبع التب على الجهة
+
+622
+00:45:16,350 --> 00:45:21,470
+التانية في هنا ال adapter هذا ال handle طبع ال ..
+
+623
+00:45:23,100 --> 00:45:27,640
+البيبت وهنا الموجود فيه ال adapter تبع التب تمام
+
+624
+00:45:27,640 --> 00:45:33,970
+ومهي المكونات كلها موجودة طيب خلينا نشوفكيف بيتم
+
+625
+00:45:33,970 --> 00:45:38,030
+تحويل حجم يعني من خلال .. يعني إما ب .. في بيبتات
+
+626
+00:45:38,030 --> 00:45:42,250
+بلف ال .. ال .. بلانجر نفسه بكون بحاركه، في بيبتات
+
+627
+00:45:42,250 --> 00:45:48,030
+بيكون لأ، في بيكون زي منظم موجود لأسفل منها و من
+
+628
+00:45:48,030 --> 00:45:51,970
+خلاله أنا بتحكم في الحجم، إذا بلفه علشان أتحكم
+
+629
+00:45:51,970 --> 00:45:56,840
+بالحجم اللي موجود هنا، شايفين؟ ها يعني تمام؟زي ما
+
+630
+00:45:56,840 --> 00:46:01,760
+انتوا شايفين بحرك بحيث ان انا اختار الحجم اللى بدى
+
+631
+00:46:01,760 --> 00:46:05,240
+طبعا ال by bit هى مكتوب هنا بتاخد من مية لألف هينا
+
+632
+00:46:05,240 --> 00:46:10,240
+ضبطناها على الف و بدى بعد هيك اروح اركبلها التب
+
+633
+00:46:10,240 --> 00:46:14,440
+الخاصة فيها طبعا لازم تكون تب مناسبة لإلها شايفين
+
+634
+00:46:14,440 --> 00:46:17,940
+التبات طبعا disposable يا بنات ال by bit التب اللى
+
+635
+00:46:17,940 --> 00:46:21,720
+بستخدمها برجعهاش مكانها بتخلص منها على طول هى التب
+
+636
+00:46:21,720 --> 00:46:29,190
+تمام هنا بيكون في هذه التب بيكون عندىsorry في هذه
+
+637
+00:46:29,190 --> 00:46:31,890
+ال tip بيكون في عندى الحجم اللى انا بدى انجله هاي
+
+638
+00:46:31,890 --> 00:46:35,830
+البلانجة طبعا انا لما اجى اكبس عليه بيفي عندى
+
+639
+00:46:35,830 --> 00:46:38,850
+first stop هاي first stop شايفين هادى اول
+
+640
+00:46:38,850 --> 00:46:42,190
+resistant بتقابلني هاي في عندى اذا هادى ال first
+
+641
+00:46:42,190 --> 00:46:47,900
+stop شايفين هنامجرد هي ضغطت فاتت عمودي في داخل
+
+642
+00:46:47,900 --> 00:46:52,680
+السائل طبعا خلينا ال tip يعني تكون مغمورة داخل
+
+643
+00:46:52,680 --> 00:46:57,400
+السائل و بنبدأ نرفع شوي شوي بالتدريج ايدنا عن
+
+644
+00:46:57,400 --> 00:47:01,540
+البلانجر ايش اللي هيصير بالظبط هيصير ان دخول
+
+645
+00:47:01,540 --> 00:47:05,220
+السائل داخل ال tip و هيك انا بكون أخدت الحجم اللي
+
+646
+00:47:05,220 --> 00:47:09,020
+موجود على ال screen طبعا لحظة انها ضغطت ضغطة واحدة
+
+647
+00:47:09,020 --> 00:47:13,660
+يعني هي ايش بتعملبتكون .. بتكون عم تعمل forward by
+
+648
+00:47:13,660 --> 00:47:17,900
+betting طبعا فهذا ال by bet او هذا ال volume بروح
+
+649
+00:47:17,900 --> 00:47:21,100
+باخده في container و بفضيه من خلال ضغطة أولى او
+
+650
+00:47:21,100 --> 00:47:24,140
+ضغطة تانية طبعا هاي شايفين الضغطة التانية هذه هي
+
+651
+00:47:24,140 --> 00:47:27,420
+الضغطة التانية طبعا هاي .. طبعا اذا انا .. انا هاي
+
+652
+00:47:27,420 --> 00:47:32,120
+هنا الضغطة بانقل ب .. باخد ال volume و بنقله لهذا
+
+653
+00:47:32,120 --> 00:47:41,050
+بفوت عمودي و لازم اتبع كل التعليمات طبعالو رجع
+
+654
+00:47:41,050 --> 00:47:48,310
+نركز عليها مرة تانية نشوف
+
+655
+00:47:48,310 --> 00:47:53,490
+هنا هى
+
+656
+00:47:53,490 --> 00:47:57,830
+هى هنا شايفين هادى الضغط
+
+657
+00:48:02,970 --> 00:48:07,490
+هي ضغطة وهي الضغطة التانية شايفين؟ هى معانا ضغطة
+
+658
+00:48:07,490 --> 00:48:13,530
+وضغطتين طبعا؟ طيب و هيك بيتم نقل حجوم بالشكل هذا
+
+659
+00:48:13,530 --> 00:48:17,800
+من الى طبعا؟ من الىال container الأصلي ل ال
+
+660
+00:48:17,800 --> 00:48:20,380
+container اللى بدى أشتغل فيه بنقل حجم اللى بديه
+
+661
+00:48:20,380 --> 00:48:25,340
+علشان أنا بكون بدى أجري تجربة معينة تمام لما باجى
+
+662
+00:48:25,340 --> 00:48:29,620
+بفضى لازم أنا أضغط على البلانجر بحيث أنه يفضى طبعا
+
+663
+00:48:29,620 --> 00:48:33,200
+البلانجر بضغط عليه بسحب بضغط عليه مرتان يدفضى و
+
+664
+00:48:33,200 --> 00:48:38,680
+هكذا يفضل يا بنات لما أنا أجى أسحب طبعا اللى أنتوا
+
+665
+00:48:38,680 --> 00:48:47,870
+شايفين هنا هي ال ..هنا شايفين كيف انا بفوت ضغطة
+
+666
+00:48:47,870 --> 00:48:51,790
+تمام لو
+
+667
+00:48:51,790 --> 00:48:52,390
+نرجع
+
+668
+00:48:55,290 --> 00:48:59,850
+تمام هى هنا فضة ال volume هى هنا ضغطة هى ضغطتين
+
+669
+00:48:59,850 --> 00:49:03,690
+تمام تعلمنا الضغطة و الضغطتين و هى هنا شايفين كده
+
+670
+00:49:03,690 --> 00:49:08,430
+أنا بفوت ضغطة و بعدين ب .. بسحب ال .. ب .. بترك
+
+671
+00:49:08,430 --> 00:49:12,630
+إيدي شوية شوية عن البلانجر مابنفع أنا أفوت مش ضغطة
+
+672
+00:49:12,630 --> 00:49:16,250
+و أضغط جوا ال solution ليش؟ لإنه ساعتها أنا بكون
+
+673
+00:49:16,250 --> 00:49:18,890
+بدخل هوا جوا ال solution إذا هو من النوع الفومي
+
+674
+00:49:18,890 --> 00:49:21,890
+اللى بيكون رغوة هيصير فيه رغوات و يصير في عندى air
+
+675
+00:49:21,890 --> 00:49:26,950
+bubble و مشاكل في نقل الحجومطبعا طيب هلأ انا بعد
+
+676
+00:49:26,950 --> 00:49:29,730
+ما انا فضيت ال volume ضغطت على البلانجر و فضيت ال
+
+677
+00:49:29,730 --> 00:49:32,350
+volume اش بده اعمل بده اضغط على ال ejector عشان
+
+678
+00:49:32,350 --> 00:49:36,370
+اتخلص من التب شايفين مجرد ما اضغط عليها ال .. ال
+
+679
+00:49:36,370 --> 00:49:40,890
+.. التب بتفصل عن ال bibert و بت .. بتم التخلص منها
+
+680
+00:49:40,890 --> 00:49:46,460
+طبعاهذا بالنسباله ال .. ال .. ال bybite اذا اول
+
+681
+00:49:46,460 --> 00:49:52,560
+حاجة بنحدد الحجم ب .. أركب ال tip بعمل rinsing pre
+
+682
+00:49:52,560 --> 00:49:58,200
+-rinsing ببللها تمام و بعدين باخد الحجم اللي بدياه
+
+683
+00:49:58,200 --> 00:50:01,960
+سواء كان باخده بطريقة ال .. ال .. ال forward
+
+684
+00:50:01,960 --> 00:50:06,000
+bybiting او ال reverse bybiting حسب اللي انا الحجم
+
+685
+00:50:06,000 --> 00:50:09,930
+اللي ال .. ال .. او السائل اللي انا بتعامل معاهو
+
+686
+00:50:09,930 --> 00:50:15,030
+بانقله من و إلى بعد هيك بتخلص من التب بهذا الشكل
+
+687
+00:50:15,030 --> 00:50:21,550
+تمام؟ ان شاء الله هيكون شغلنا على ال .. ال bybit و
+
+688
+00:50:21,550 --> 00:50:24,630
+طبعا معظم .. كل الفحوصات بتنعمل باستخدام ال bybit
+
+689
+00:50:24,630 --> 00:50:28,630
+انا بدي اضيف حجم من ال reagent و حجم من العينة حجم
+
+690
+00:50:28,630 --> 00:50:31,510
+من ال standard و حجم من ال reagent كل شغلي
+
+691
+00:50:31,510 --> 00:50:33,710
+باستخدام ال bybit اذا هذه صديقتنا هذه اللي
+
+692
+00:50:33,710 --> 00:50:36,390
+هترافقنا من شاء الله من سنة تانية لسنة رابعة لجهة
+
+693
+00:50:36,390 --> 00:50:39,950
+الأداء فبالتالي لازم نكون دقيقين في استخدامهاو
+
+694
+00:50:39,950 --> 00:50:42,690
+عشان .. عشان نكون دقيقين باستخدامها و تكون ال vibe
+
+695
+00:50:42,690 --> 00:50:46,370
+تهدي احنا بنعمللها صيانة و بنضمن دقتها باستمرار
+
+696
+00:50:46,370 --> 00:50:49,530
+لازم انه انا اعمللها calibration اش يعني
+
+697
+00:50:49,530 --> 00:50:52,890
+calibration يعني اعادة ضبط وتهيئة علشان اتأكد من
+
+698
+00:50:52,890 --> 00:50:57,070
+شغلها ان هي عم تاخدلي شغل دقيق طبعا كيف انا بدي
+
+699
+00:50:57,070 --> 00:51:01,660
+اخد ال .. ال .. اكيد اعمللها calibration؟بدي
+
+700
+00:51:01,660 --> 00:51:06,200
+استخدمها يعني او اظبط شغلها او اتأكد من انها عم
+
+701
+00:51:06,200 --> 00:51:10,820
+تاخد volumes بشكل دقيق باستخدام الحجوم الماء ال
+
+702
+00:51:10,820 --> 00:51:14,960
+distilled water تمام؟ هنا بيحكيلي انه تحت درجة
+
+703
+00:51:14,960 --> 00:51:21,200
+الحرارة و الضغط الثابت الكثافة الماء أيضا ثابتة
+
+704
+00:51:21,200 --> 00:51:26,860
+فالحجم من الماء تمام ممكن انا يعني one ml من الميه
+
+705
+00:51:26,860 --> 00:51:31,820
+بيعادل one gramمن الميه تمام ال .. ال one ml ال
+
+706
+00:51:31,820 --> 00:51:36,360
+volume one ml لو أنا جيت وزنته تحت درجة حرارة وضغط
+
+707
+00:51:36,360 --> 00:51:40,400
+ثابت وكثافة ثابتة للماء فهي .. ال .. ال one ml
+
+708
+00:51:40,400 --> 00:51:46,710
+هيعطيني one gram من ال water تمام؟ طيب فبالتاليال
+
+709
+00:51:46,710 --> 00:51:51,330
+.. ال calibration لل بايبت لازم انا اكون بس يعني
+
+710
+00:51:51,330 --> 00:51:54,390
+بدي اعرف ال volume اللي عم تاخده حجم الماء اللي
+
+711
+00:51:54,390 --> 00:51:59,890
+بتاخده و هل هو accurate او precise و طبعا هذا
+
+712
+00:51:59,890 --> 00:52:06,910
+بيتأثر بدرجة حرارة الغرفة و ايضا الضغط الجوي و
+
+713
+00:52:06,910 --> 00:52:10,190
+الرطوبة نسبة الرطوبة اللي موجودة في الوسط او في
+
+714
+00:52:10,190 --> 00:52:14,320
+البيئة المحيطة لإجراء التجربةعشان هيك انا بستخدم
+
+715
+00:52:14,320 --> 00:52:17,980
+factor او ثابت اسمه z factor في الحسابات طبعتي
+
+716
+00:52:17,980 --> 00:52:23,340
+هنحكي عنه كمان شوية هذا ال z factor فالفكرة طبعتي
+
+717
+00:52:23,340 --> 00:52:27,660
+انه انا بدي اخد one ml من ال distilled water يعني
+
+718
+00:52:27,660 --> 00:52:30,980
+بدي اجيب ال by bit بدي اعمللها setting بدي ا ..
+
+719
+00:52:30,980 --> 00:52:35,700
+اضب .. اضبطها على one ml اركب tip اخد اعمل ال pre
+
+720
+00:52:35,700 --> 00:52:39,920
+-rinsing ابلل ال tip من .. و اتخلص من ال solution
+
+721
+00:52:39,920 --> 00:52:44,350
+اللي أخدته في المرة الأولىبعد هيك إيش بدي أعمل؟
+
+722
+00:52:44,350 --> 00:52:50,110
+بدي أخد one ml من distilled water و أحطه في cup ال
+
+723
+00:52:50,110 --> 00:52:53,990
+cup هذا بدي أكون مسفر عليه في الميزان بدي أسفر
+
+724
+00:52:53,990 --> 00:52:57,590
+عليه و أحط ال one ml و أشوف الوزنة تبعتي الوزنة
+
+725
+00:52:57,590 --> 00:53:00,090
+تبعتي هتكون إذا هو مسفر على ال cup فالوزنة اللي
+
+726
+00:53:00,090 --> 00:53:05,050
+هتطلع معايا هي وزنت ال one ml المفروض تكون مقاربة
+
+727
+00:53:05,050 --> 00:53:08,470
+ل one gram تمام؟ المفروض انها تكون مقاربة ل one
+
+728
+00:53:08,470 --> 00:53:13,310
+gram او .. او يعني exactly one gramطبعا فعشان ان
+
+729
+00:53:13,310 --> 00:53:16,130
+احنا عندنا درجة الحرارة و الدغط الجوي و ال
+
+730
+00:53:16,130 --> 00:53:21,070
+humidity متغيرة فانا بروح بدرب الوزن هذه تبعتي بال
+
+731
+00:53:21,070 --> 00:53:25,390
+Z factor و في جدول في اخر ال .. ال .. ال slide
+
+732
+00:53:25,390 --> 00:53:29,590
+بعلمني كيف انا اختار ال Z factor المناسب اللي بدي
+
+733
+00:53:29,590 --> 00:53:36,880
+ادرب في القراءات تبعتي طبعا فإذا ال ..الحجم هي هنا
+
+734
+00:53:36,880 --> 00:53:41,300
+مثلا إيش بلزمني sorry إيش بلزمني لأ التجربة تبعتي؟
+
+735
+00:53:41,300 --> 00:53:46,100
+بلزمني by bit و tip بلزمني beaker أو cup لحتى أحطه
+
+736
+00:53:46,100 --> 00:53:49,220
+على الميزان و أصفر عليه تمام؟ بلزمني distilled
+
+737
+00:53:49,220 --> 00:53:54,260
+water بلزمني أقيس درجة الحرارة و ميزان و أقيس ال
+
+738
+00:53:54,260 --> 00:54:00,850
+pressure الدغط عشان أطلع z factorأول إشي بدي أقيس
+
+739
+00:54:00,850 --> 00:54:07,510
+درجة حرارة الماء و أسجلها بعد هيك باستخدام beaker
+
+740
+00:54:07,510 --> 00:54:10,950
+طبعا انه انا بدي أحطه على ال .. طبعا مش راح أقرأ
+
+741
+00:54:10,950 --> 00:54:18,900
+كل الخطوات هايراح اختصرها باستخدام ال beaker او ال
+
+742
+00:54:18,900 --> 00:54:23,080
+cup راح احطه على ال analytical balance و راح هصفر
+
+743
+00:54:23,080 --> 00:54:27,080
+عليه طبعا دي خلي ا .. ا .. اخلي الميزان يعتبر ان
+
+744
+00:54:27,080 --> 00:54:32,040
+وزن ال cup هذا zero طبعابعد هيك بدي أعمل pre ..
+
+745
+00:54:32,040 --> 00:54:35,520
+بدي أركب تب للبيبط و بعد ما أكون أنا حددت الحجم
+
+746
+00:54:35,520 --> 00:54:40,840
+المناسب لإلها اللي هو one ml يعني الف microliter و
+
+747
+00:54:40,840 --> 00:54:44,940
+بعد هيك بدي أعمل pre-rinsing عرفنا إيش يعني pre
+
+748
+00:54:44,940 --> 00:54:49,040
+-rinsing يعني أنا بدي أسحب أسبيرات and spins يعني
+
+749
+00:54:49,040 --> 00:54:53,640
+بدي أسحب و أتخلص من الحجم على أساس ممكن أعيد هذه
+
+750
+00:54:53,640 --> 00:54:56,860
+الخطوة تلت مرات على أساس أتأكد أنه ال .. ال ..
+
+751
+00:54:56,860 --> 00:54:59,580
+التب صارت مبللة من الداخل يعني مش هيكون في عندى لا
+
+752
+00:54:59,580 --> 00:55:05,320
+.. لا فقطولا error في ال volume تمامو بعد هيك إيش
+
+753
+00:55:05,320 --> 00:55:09,880
+بدي أعمل؟ بدي أضغط على ال .. ال .. ال .. ال .. ال
+
+754
+00:55:09,880 --> 00:55:11,120
+.. ال .. ال .. ال .. ال .. ال .. ال .. ال .. ال ..
+
+755
+00:55:11,120 --> 00:55:11,380
+ال .. ال .. ال .. ال .. ال .. ال .. ال .. ال .. ال
+
+756
+00:55:11,380 --> 00:55:11,580
+.. ال .. ال .. ال .. ال .. ال .. ال .. ال .. ال ..
+
+757
+00:55:11,580 --> 00:55:13,040
+ال .. ال .. ال .. ال .. ال .. ال .. ال .. ال .. ال
+
+758
+00:55:13,040 --> 00:55:13,160
+.. ال .. ال .. ال .. ال .. ال .. ال .. ال .. ال ..
+
+759
+00:55:13,160 --> 00:55:13,660
+ال .. ال .. ال .. ال .. ال .. ال .. ال .. ال .. ال
+
+760
+00:55:13,660 --> 00:55:13,660
+.. ال .. ال .. ال .. ال .. ال .. ال .. ال .. ال ..
+
+761
+00:55:13,660 --> 00:55:14,400
+ال .. ال .. ال .. ال .. ال .. ال .. ال .. ال .. ال
+
+762
+00:55:14,400 --> 00:55:21,420
+.. ال .. ال ..
+
+763
+00:55:21,420 --> 00:55:21,420
+ال .. ال .. ال .. ال .. ال .. ال .. ال .. ال .. ال
+
+764
+00:55:21,420 --> 00:55:21,420
+.. ال .. ال .. ال .. ال .. ال .. ال .. ال .. ال ..
+
+765
+00:55:21,420 --> 00:55:21,440
+ال .. ال .. ال .. ال .. ال .. ال .. ال .. ال .. ال
+
+766
+00:55:21,440 --> 00:55:25,140
+.. ال ..
+
+767
+00:55:34,190 --> 00:55:40,910
+عشان السائل يفوت داخل التب أيضا بشكل متأني يعني
+
+768
+00:55:40,910 --> 00:55:44,210
+انه مايصير فيه اندفاع لا solution و هي air bubble
+
+769
+00:55:44,210 --> 00:55:48,260
+و المشاكل اللي ممكن تحدث في البيت انجلتمام؟ هيك
+
+770
+00:55:48,260 --> 00:55:51,980
+أنا أخدت الحجم اللي بدياه بروح بفضيه في ال beaker
+
+771
+00:55:51,980 --> 00:55:55,340
+أو ال cup اللي موجود على الميزان و باخد القراءة
+
+772
+00:55:55,340 --> 00:55:59,760
+تمام؟ بعد هيك ممكن أنا أرجع أصفر على ال cup و ال
+
+773
+00:55:59,760 --> 00:56:03,420
+.. ال volume اللي موجود فيه أو أن أنا أستخدم cup
+
+774
+00:56:03,420 --> 00:56:08,100
+جديد إيه للخيار؟بسفر و بعيد الخطوة مرة تانية باخد
+
+775
+00:56:08,100 --> 00:56:13,560
+one amal بالبيبت و بفضيه و باخد كمان وزنة و كمان
+
+776
+00:56:13,560 --> 00:56:17,340
+مرة تالتة باخد one amal و بفضيه في الميزان و باخد
+
+777
+00:56:17,340 --> 00:56:22,320
+وزنة تالتة فإذا صار في عندي تلت وزنات لتلت حجوم
+
+778
+00:56:22,320 --> 00:56:25,400
+تمام فإيش بدي أعمل في التلت وزنات هدول؟ بدي أجيب
+
+779
+00:56:25,400 --> 00:56:28,740
+ال average تبعهم يعني بدي أجيب مجموعهم على تلتة
+
+780
+00:56:29,100 --> 00:56:34,400
+تمام؟ ال .. هذه ال ..ش اسمه؟ ال .. يعني تكملت
+
+781
+00:56:34,400 --> 00:56:37,220
+التجربة اللي انا حكيتها يعني انه .. انه عيدي
+
+782
+00:56:37,220 --> 00:56:41,380
+الخطوات تلت مرات و جيبي القراءة هي هنا مثلا
+
+783
+00:56:41,380 --> 00:56:44,340
+بيحكيلي ممكن تعديها اربعتاشر مرات حسب يعني ممكن
+
+784
+00:56:44,340 --> 00:56:48,200
+تعديها تلت مرات اربع مرات خمس مرات المهم بالنهاية
+
+785
+00:56:48,200 --> 00:56:51,640
+الوزنات اللي تطلع معاكي بدك تاخديلها average ايش
+
+786
+00:56:51,640 --> 00:56:56,440
+يعني average؟ يعني مجموعها على عددهاو اطلع قراءة
+
+787
+00:56:56,440 --> 00:56:59,680
+واحدة القراءة واحدة هي هذه اللي بدك تروحي اتعاوضي
+
+788
+00:56:59,680 --> 00:57:02,940
+فيها بالقانون ال weight او ال average of weight
+
+789
+00:57:02,940 --> 00:57:06,180
+اللي طلع معاكي و بدك تضربيه في ال z factor اللي هو
+
+790
+00:57:06,180 --> 00:57:10,980
+معامل اللي هو الحرارة و الضغط تمام اللي موجود عندك
+
+791
+00:57:10,980 --> 00:57:14,980
+و بالنهاية بحسب ال volumeانا خدت ال bybit كانت
+
+792
+00:57:14,980 --> 00:57:18,940
+بتحكيلي ان انا باخد one ml على الشاشة تبع ال bybit
+
+793
+00:57:18,940 --> 00:57:22,840
+مكتوب one ml فالمفروض ان هذا الكلام ال wet في ال
+
+794
+00:57:22,840 --> 00:57:27,020
+zip factor يكون one ml ايضا يعني او مقارب ل one ml
+
+795
+00:57:27,020 --> 00:57:34,050
+فممكن اناأطبق قوانين التحليلية يعني هي ان انا بجيب
+
+796
+00:57:34,050 --> 00:57:39,050
+ال average لقراءات الوزنة اللي هي مجموع القراءات
+
+797
+00:57:39,050 --> 00:57:43,170
+على عددها تمام ممكن اطبق ايضا احسب ال standard
+
+798
+00:57:43,170 --> 00:57:47,870
+deviation اجيب ال accuracy يعني القيمة الوزنة اللي
+
+799
+00:57:47,870 --> 00:57:50,610
+طلعت معايا و اللي المفروض تطلع مثلا طلعت معايا
+
+800
+00:57:50,610 --> 00:57:54,690
+point 999 الوزنة و المفروض انها تطلع one milligram
+
+801
+00:57:54,690 --> 00:57:58,280
+بحسبهابس شوف .. شوف نسبة ال accuracy تبعت القيمة
+
+802
+00:57:58,280 --> 00:58:02,100
+تبعتي و ال coefficient of variation اللي هي ..
+
+803
+00:58:02,100 --> 00:58:04,560
+اللي هي عبارة عن ال standard deviation على ال mean
+
+804
+00:58:04,560 --> 00:58:11,920
+ضارب 100% لازم ال accuracy هذه تطلع من 99 ل 101% و
+
+805
+00:58:11,920 --> 00:58:16,470
+لازمال coefficient of variation يكون اقل من واحد
+
+806
+00:58:16,470 --> 00:58:19,690
+في المية هذا يكون اقل من واحد في المية عشان اقول
+
+807
+00:58:19,690 --> 00:58:22,290
+ان ال by bit هذه accurate و precise و بتعطيني
+
+808
+00:58:22,290 --> 00:58:25,570
+volumes دقيقة و الحجم اللي انا باخده هو نفسه اللي
+
+809
+00:58:25,570 --> 00:58:28,990
+موجود على الشاشة و شغلها مية لمية اذا انا لما بدي
+
+810
+00:58:28,990 --> 00:58:31,930
+اجري افحصات و بدي انقل volume باستخدام هذه ال by
+
+811
+00:58:31,930 --> 00:58:36,740
+bit اذا انا عم بشتغل بشكل صحيح و بشكل دقيقطبعا هذا
+
+812
+00:58:36,740 --> 00:58:40,740
+الجدول اللى انا من خلاله بختار مثلا ال .. ال z
+
+813
+00:58:40,740 --> 00:58:45,180
+value او اللى هو معامل ال .. الحرارة و الضغط طبعا
+
+814
+00:58:45,180 --> 00:58:47,980
+ال z value مثلا انا بشوف ايش الضغط اللى موجود عندى
+
+815
+00:58:47,980 --> 00:58:50,040
+حسب المنطقة اللى انا .. اللى انا فيها لان افرض
+
+816
+00:58:50,040 --> 00:58:54,620
+مثلا ان انا موجودة في منطقة ضغطها الف و ست .. سبعة
+
+817
+00:58:54,620 --> 00:58:59,420
+و ستين ملي بار طبعاو درجة الحرارة عندي خمسة و
+
+818
+00:58:59,420 --> 00:59:04,240
+عشرين فبروح هنا هي خمسة و عشرين بمشي لعند مين لعند
+
+819
+00:59:04,240 --> 00:59:08,100
+ال .. ال .. ال .. المنطقة اللي هي ال .. اللي فيها
+
+820
+00:59:08,100 --> 00:59:11,600
+الضغط تبعي تمام؟ اللي فيها الضغط تبعي هنا هيك هي
+
+821
+00:59:11,600 --> 00:59:15,480
+هذه القيمة تقاطع درجة الحرارة اللي بديها مع الضغط
+
+822
+00:59:15,480 --> 00:59:18,480
+اللي .. اللي بديها هي هذه قيمة ال Z factor اللي
+
+823
+00:59:18,480 --> 00:59:22,300
+بروح ب .. ب .. أطبق فيهاتمام هي هذه قيمة ال z
+
+824
+00:59:22,300 --> 00:59:26,060
+factor اللي بروح بطبق فيها و بحسب حسابات تبعتي زي
+
+825
+00:59:26,060 --> 00:59:29,820
+ما حكينا لو انا عديت التجربة مرة مرتين تلاتة اربع
+
+826
+00:59:29,820 --> 00:59:34,900
+عشرة و حصلت على مثلا عشر قيم باخد ال average تبعهم
+
+827
+00:59:34,900 --> 00:59:39,100
+بدربهم بال z factor بحصل على ال volume و بطبق في
+
+828
+00:59:39,100 --> 00:59:43,180
+القوانين تبعتي القيمة اللي انا جستها على القيمة
+
+829
+00:59:43,180 --> 00:59:46,570
+اللي المفروض تطلعضرب 100% بحسب ال accuracy بحسب ال
+
+830
+00:59:46,570 --> 00:59:50,450
+coefficient of variation بشوف نسبة دقة و صحة ال ..
+
+831
+00:59:50,450 --> 00:59:54,070
+ال vibe بتاعي و بحكم على شغلها و هل انها محتاجة
+
+832
+00:59:54,070 --> 01:00:00,690
+انها يتم تصليحها او انه لأ مناسبة و تستخدم يعني
+
+833
+01:00:00,690 --> 01:00:05,350
+موثوقة للاستخدام تمام؟بهيك بنكون خلصنا محاضرتنا
+
+834
+01:00:05,350 --> 01:00:10,030
+لليوم ان شاء الله تكون هيك الاشياء واضحة حولت ان
+
+835
+01:00:10,030 --> 01:00:15,350
+انا اورجيكم صور اورجيكم فيديو لكيف ال by بتاعي كنت
+
+836
+01:00:15,350 --> 01:00:18,810
+نستخدمها و تعليمات اللي بنتابعها أثناء ال by
+
+837
+01:00:18,810 --> 01:00:25,490
+bettingلعسى انه يعني تكون الاشي مش مبهم لقلكم لإنه
+
+838
+01:00:25,490 --> 01:00:29,810
+يعني احنا مقدرين انه بداية التخصص لازم تكون ..
+
+839
+01:00:29,810 --> 01:00:35,650
+تكون يعني كل إشي سهل ومستثار لحتى تتمكنوا من الفهم
+
+840
+01:00:35,650 --> 01:00:40,430
+المعلومة وإدراكها يعطيكم ألف عافية وشكرا لكم
+
diff --git a/PL9fwy3NUQKwY9jl8xw-bWkVZkWcGGyspd/Up-Q0W10hks.srt b/PL9fwy3NUQKwY9jl8xw-bWkVZkWcGGyspd/Up-Q0W10hks.srt
new file mode 100644
index 0000000000000000000000000000000000000000..dc466c9707d56b429fb323a2642a91fae026f92b
--- /dev/null
+++ b/PL9fwy3NUQKwY9jl8xw-bWkVZkWcGGyspd/Up-Q0W10hks.srt
@@ -0,0 +1,1874 @@
+1
+00:00:10,020 --> 00:00:13,720
+السلام عليكم ورحمة الله وبركاته طالبات العزيزات
+
+2
+00:00:13,720 --> 00:00:17,940
+اليوم إن شاء الله راح نتحدث عن red blood cell
+
+3
+00:00:17,940 --> 00:00:23,000
+مؤشرات خلايا الدم الحمراء أو معاملات خلايا
+
+4
+00:00:23,000 --> 00:00:26,760
+الدم الحمراء في البداية خلينا نعرف شو يعني red
+
+5
+00:00:26,760 --> 00:00:30,560
+blood cell indices. Red blood cell indices هي عبارة
+
+6
+00:00:30,560 --> 00:00:35,120
+عن measurements قياسات مؤشرات أو معاملات that
+
+7
+00:00:35,120 --> 00:00:39,840
+describe the size and oxygen-carrying protein
+
+8
+00:00:39,840 --> 00:00:44,700
+content of the red blood cell. إذن هي معاملات ومؤشرات
+
+9
+00:00:44,700 --> 00:00:50,480
+بتصف حجم خلية الـ RBCs بالإضافة إلى محتوها من الـ
+
+10
+00:00:50,480 --> 00:00:53,620
+hemoglobin اللي هو عبارة عن الـ oxygen-carrying
+
+11
+00:00:53,620 --> 00:00:57,240
+protein البروتين المسؤول عن حمل الـ oxygen ونقله
+
+12
+00:00:57,740 --> 00:01:02,600
+تمام؟ إذا red blood cell indices هي مؤشرات تصف حجم
+
+13
+00:01:02,600 --> 00:01:07,400
+الخلية ومحتوها من الـ hemoglobin. وإذا بتتذكروا لما
+
+14
+00:01:07,400 --> 00:01:11,960
+شرحنا محاضرة الـ blood film، حكينا إنه من خلال الـ
+
+15
+00:01:11,960 --> 00:01:14,640
+blood film أو الـ blood smear أنا بقيم الـ
+
+16
+00:01:14,640 --> 00:01:19,220
+morphology، الـ shape للخلية، وحكيت لكم بالنسبة للـ RBCs
+
+17
+00:01:19,220 --> 00:01:24,200
+أنا بدرس... بشوف حجمها، بدرس أيضًا محتوها من الـ
+
+18
+00:01:24,200 --> 00:01:27,280
+hemoglobin، بشوفها خلايا فاضية أو فيها hemoglobin،
+
+19
+00:01:27,280 --> 00:01:30,600
+الـ central pallor اللي... اللي هو شحوب المركز اللي
+
+20
+00:01:30,600 --> 00:01:35,100
+في داخلها، هل يحتله طولها أو أكثر من ذلك، وكل هذه
+
+21
+00:01:35,100 --> 00:01:40,640
+لها مؤشرات ومرتبطة بتشخيص الأنيميا، تمام؟ إذا الـ
+
+22
+00:01:40,640 --> 00:01:44,440
+red blood cell indices أيضًا هي عبارة عن عوامل
+
+23
+00:01:44,440 --> 00:01:51,600
+ومؤشرات، يعني حسابات، قياسات أيضًا بتدلّني عن حجم
+
+24
+00:01:51,600 --> 00:01:56,480
+الخلية ومحتوها من الـ hemoglobin، تمام؟ وبالتالي إذا
+
+25
+00:01:56,480 --> 00:02:01,660
+تستخدم بالإضافة للـ blood film، بتستخدم to help in
+
+26
+00:02:01,660 --> 00:02:05,820
+the differential diagnosis of anemia، إذا تساعدني
+
+27
+00:02:05,820 --> 00:02:10,420
+لحتى أفرّق ما بين أنواع الأنيميا وأشخص نوع
+
+28
+00:02:10,420 --> 00:02:15,980
+الأنيميا الصحيح أو الدقيق، تمام؟ The relationships
+
+29
+00:02:15,980 --> 00:02:20,700
+between hematocrit, hemoglobin level and RBCs
+
+30
+00:02:20,700 --> 00:02:25,020
+count are converted to blood cell indices through
+
+31
+00:02:25,020 --> 00:02:29,320
+mathematical formulas، تمام؟ احنا حكينا أنا بقيم
+
+32
+00:02:29,320 --> 00:02:33,360
+حجم الخلية، محتواها من الـ hemoglobin بالـ blood film
+
+33
+00:02:33,360 --> 00:02:36,820
+عن طريق الـ morphology، شكلها، طبعًا أنا بحفظ شكلها
+
+34
+00:02:36,820 --> 00:02:41,820
+خلية RBCs، تلوينها، hemoglobin، تلوينها، Central pallor
+
+35
+00:02:41,820 --> 00:02:46,680
+و بيصير حجمها، مقلوبها، إللي طبعًا بالنسبة للـ Red blood
+
+36
+00:02:46,680 --> 00:02:50,000
+cell indices، كيف أنا بقيم الـ size و الـ hemoglobin
+
+37
+00:02:50,000 --> 00:02:54,630
+content من خلال حسابات، من خلال mathematical formulas،
+
+38
+00:02:54,630 --> 00:03:00,510
+صياغات رياضية، تمام؟ أستخدم فيها أو تم استخدام فيها الـ
+
+39
+00:03:00,510 --> 00:03:04,850
+hematocrit و الـ hemoglobin level و الـ RBCs، إذا تم
+
+40
+00:03:04,850 --> 00:03:10,390
+توظيف الـ hematocrit، الـ hemoglobin level، وأيضًا الـ
+
+41
+00:03:10,390 --> 00:03:16,910
+RBCs count لحتى اللي بتم صياغة معادلات حسابية بتأشر
+
+42
+00:03:16,910 --> 00:03:21,930
+أو بتدل أو بتعتبر كمعاملات تعبر عن حجم الخلية
+
+43
+00:03:21,930 --> 00:03:27,690
+ومحتواها من الـ hemoglobin، تمام؟ إذا لنرتّب أفكارنا،
+
+44
+00:03:27,690 --> 00:03:31,770
+red blood cell indices هي عبارة عن قياسات أو
+
+45
+00:03:31,770 --> 00:03:36,890
+مؤشرات أو معاملات تعبر عن حجم الخلية ومحتواها من الـ
+
+46
+00:03:36,890 --> 00:03:40,050
+hemoglobin، بتفيدني لحتى أعمل differential
+
+47
+00:03:40,050 --> 00:03:45,840
+diagnosis للأنيميا، تمام؟ وكيف أنا بحصل على هاي
+
+48
+00:03:45,840 --> 00:03:49,960
+القياسات أو هاي المؤشرات؟ تم توظيف الـ hemoglobin و
+
+49
+00:03:49,960 --> 00:03:53,300
+الـ level أو الـ hemoglobin concentration و الـ
+
+50
+00:03:53,300 --> 00:03:58,440
+hematocrit و الـ RBC count من خلال صيغ... صيغ
+
+51
+00:03:58,440 --> 00:04:04,820
+رياضية، من خلال معرفة العلاقة ما بين هاي ال... ال...
+
+52
+00:04:04,820 --> 00:04:08,340
+المكونات أو هاي الحسابات، الـ hematocrit والـ
+
+53
+00:04:08,340 --> 00:04:13,080
+hemoglobin level و الـ RBC count، تم صياغة ال... الـ
+
+54
+00:04:13,080 --> 00:04:16,300
+indices، هاي، طب شو هي الـ indices هاي؟ المؤشرات هاي
+
+55
+00:04:16,300 --> 00:04:20,320
+عبارة عن إيه؟ هي ثلاث مؤشرات: الـ mean corpuscular
+
+56
+00:04:20,320 --> 00:04:26,190
+volume، و الـ MCV، و الـ mean corpuscular hemoglobin، و
+
+57
+00:04:26,190 --> 00:04:30,210
+الـ mean corpuscular hemoglobin concentration، MCV،
+
+58
+00:04:30,210 --> 00:04:36,630
+MCH، MCHC، طبعًا، طيب الـ mean، متوسط معناها، corpuscular
+
+59
+00:04:36,630 --> 00:04:42,450
+يعني... يعني بتعود على كلمة كرة لأن احنا بنحكي عن
+
+60
+00:04:42,450 --> 00:04:46,790
+خلايا الدم الحمراء، كريات الدم الحمراء، وكريات الدم
+
+61
+00:04:46,790 --> 00:04:50,470
+البيضاء، طبعًا، فإذا corpuscular يعني جاية من... من
+
+62
+00:04:50,470 --> 00:04:54,750
+...من... من شيء كروي، أي يعبر عن الخلية، تمام؟
+
+63
+00:04:54,750 --> 00:04:58,510
+يعني هو متوسط، إذا mean corpuscular volume هاي...
+
+64
+00:04:58,510 --> 00:05:03,090
+هاي، معناها متوسط حجم الخلية، mean
+
+65
+00:05:03,090 --> 00:05:07,570
+corpuscular hemoglobin، متوسط حجم ال... أو متوسط
+
+66
+00:05:07,570 --> 00:05:12,050
+سوري، الـ hemoglobin content للخلية أو الكرية،
+
+67
+00:05:12,050 --> 00:05:15,130
+تمام؟ و الـ mean corpuscular hemoglobin
+
+68
+00:05:15,130 --> 00:05:19,330
+concentration، متوسط تركيز الـ hemoglobin في الكرية
+
+69
+00:05:19,330 --> 00:05:26,640
+أو في الخلية، تمام؟ إذا MCV, MCH, MCHC، هدول هم الـ
+
+70
+00:05:26,640 --> 00:05:31,060
+indices، هدول هم المؤشرات والقياسات اللي لهم صياغ
+
+71
+00:05:31,060 --> 00:05:35,740
+ومعادلات حسابية باستخدام الـ hematocrit و الـ
+
+72
+00:05:35,740 --> 00:05:40,140
+hemoglobin level و RBC count، أنا ممكن أحسبهم، تمام؟
+
+73
+00:05:40,140 --> 00:05:43,920
+وبيدلّوني على حجم الخلية وعلى محتوها من الـ
+
+74
+00:05:43,920 --> 00:05:49,210
+hemoglobin، وهذا بساعدني بتشخيص الأنيميا، تمام؟ نيجي
+
+75
+00:05:49,210 --> 00:05:54,450
+للـ bare bones، الغرض من هاي الـ indices، في البداية
+
+76
+00:05:54,450 --> 00:05:59,630
+نعرف إيش يعني anemia. Anemia includes a variety of
+
+77
+00:05:59,630 --> 00:06:04,610
+conditions with the same outcome، يعني ال... الـ
+
+78
+00:06:04,610 --> 00:06:11,330
+anemia ممكن يكون أسبابها عديدة، ممكن يكون سببها إمّا
+
+79
+00:06:11,330 --> 00:06:18,130
+نقص في تصنيع الـ hemoglobin، نقص في الـ iron، الخلل في
+
+80
+00:06:18,130 --> 00:06:23,810
+تصنيع الـ hemoglobin، حتى كمان ممكن يكون shortage أو
+
+81
+00:06:23,810 --> 00:06:29,450
+حتى الـ life span، يعني قصر في عمر الخلية، تمام؟ الـ
+
+82
+00:06:29,450 --> 00:06:35,170
+life span قليلة للـ RBCs، ممكن يكون
+
+83
+00:06:38,790 --> 00:06:45,690
+الخلية تفتقد عنصر الـ folic acid أو الـ vitamin B12،
+
+84
+00:06:45,690 --> 00:06:49,550
+اللي بيلزمها لحتى تنقسم داخل الـ bone marrow وتنضج
+
+85
+00:06:49,550 --> 00:06:54,410
+وتخرج إلى الـ circulation، تمام؟ إذا ممكن إن الـ RBC
+
+86
+00:06:54,410 --> 00:06:58,550
+بتتصنع طبيعي، عددها طبيعي، و الـ hemoglobin تبعها
+
+87
+00:06:58,550 --> 00:07:03,560
+طبيعي، بس لما بتطلع على ال... الـ circulation بتتكسر
+
+88
+00:07:03,560 --> 00:07:10,380
+بسرعة، إذا عندي أسباب عديدة لأن... نحكي عنها إنها
+
+89
+00:07:10,380 --> 00:07:14,220
+بتسبب لي أنيميا، تمام؟ إذا إن هي عبارة عن variety of
+
+90
+00:07:14,220 --> 00:07:19,340
+conditions، في ظروف عديدة ممكن تسبب لي أنيميا، ولكن
+
+91
+00:07:19,340 --> 00:07:24,840
+المحصلة كلها يتم وصفها بأنه الـ blood can't carry
+
+92
+00:07:24,840 --> 00:07:29,350
+as much oxygen as it should، ال... ال... الـ blood
+
+93
+00:07:29,350 --> 00:07:33,890
+كمية الدم و كمية الـ RBCs اللي موجودة لا تحمل
+
+94
+00:07:33,890 --> 00:07:39,130
+أكسجين كافي إلى الـ tissues، تمام؟ كما ينبغي، as it
+
+95
+00:07:39,130 --> 00:07:44,390
+should، فبالتالي الشخص بيبدأ يعاني من hypoxia، إيش
+
+96
+00:07:44,390 --> 00:07:49,040
+يعني hypoxia؟ يعني نقص أكسجين في الجسم، السبب إنه
+
+97
+00:07:49,040 --> 00:07:52,840
+إمّا الـ RBCs عمرها قصير أو بتتكسر بسرعة أو بيتم
+
+98
+00:07:52,840 --> 00:07:58,320
+تصنيعها بشكل مش كافي، الـ hemoglobin بيتصنع بشكل
+
+99
+00:07:58,320 --> 00:08:02,420
+قليل، أو يعني بتركيز قليل، أو حتى كمان بيتصنعوا في
+
+100
+00:08:02,420 --> 00:08:06,640
+خطأ في تصنيعه، ما فيش iron كفاية للـ hemoglobin، لأنه
+
+101
+00:08:06,640 --> 00:08:09,620
+حكينا الـ iron أساسي لتركيب الـ hemoglobin اللي هو
+
+102
+00:08:09,620 --> 00:08:13,600
+أصلًا أساسي لحمل الـ oxygen، تمام؟ الخلايا وهي عم
+
+103
+00:08:13,600 --> 00:08:18,120
+تنقسم، صار فيها خلل معين، هذه كلها conditions، تمام؟
+
+104
+00:08:18,120 --> 00:08:22,680
+المحصلة hypoxia، إذًا، وبيصير إن الـ blood can't
+
+105
+00:08:22,680 --> 00:08:28,000
+carry oxygen as much as it should، تمام؟ أمّا الـ
+
+106
+00:08:28,000 --> 00:08:31,660
+healthy person، الشخص الطبيعي، الـ normal has
+
+107
+00:08:31,660 --> 00:08:35,260
+adequate number of correctly sized red blood cells
+
+108
+00:08:35,260 --> 00:08:38,700
+that contain enough hemoglobin to carry sufficient
+
+109
+00:08:38,700 --> 00:08:44,150
+oxygen to all the body's tissues، تمام؟ الشخص الطبيعي
+
+110
+00:08:44,150 --> 00:08:48,830
+عنده adequate number، عدد طبيعي وكافي من الـ RBCs،
+
+111
+00:08:48,830 --> 00:08:52,610
+اللي حجمها طبيعي واللي محتوها من الـ hemoglobin
+
+112
+00:08:52,610 --> 00:08:56,850
+أيضًا كافي، فبالتالي هي بتحمل oxygen sufficient،
+
+113
+00:08:56,850 --> 00:09:02,490
+كافي للـ all body tissues، لكل أنسجة الجسم، تمام؟ An
+
+114
+00:09:02,490 --> 00:09:06,970
+anemic person has red blood cells that are either
+
+115
+00:09:06,970 --> 00:09:11,190
+too small or too few in number، الشخص اللي... اللي
+
+116
+00:09:11,190 --> 00:09:15,270
+...اللي بنحكي عنه anemic، عنده أنيميا، ال... الـ red
+
+117
+00:09:15,270 --> 00:09:18,510
+blood cells تبعته ممكن تكون صغيرة، حجمها صغير كثير،
+
+118
+00:09:18,510 --> 00:09:21,630
+فبالتالي محتواها من الـ hemoglobin هيكون قليل، أو
+
+119
+00:09:21,630 --> 00:09:28,250
+ممكن يكون عددها قليل، طبعًا إمّا نتيجة ضعف الإنتاج أو
+
+120
+00:09:28,250 --> 00:09:32,050
+حتى ممكن لأنها الـ life span، حكينا فيها shortage،
+
+121
+00:09:32,050 --> 00:09:35,550
+قصيرة، متوسط عمر الخلية، تطلع على الـ circulation
+
+122
+00:09:35,550 --> 00:09:41,860
+تتكسر بسرعة، طبعًا بيصير لها lysis، فـ whatever السبب
+
+123
+00:09:41,860 --> 00:09:46,980
+في النهاية هذا كله بينوصف كـ anemia، الأنيميا ممكن
+
+124
+00:09:46,980 --> 00:09:53,540
+تكون كمرض منفصل بحد ذاته، أو ممكن تكون عرض لمرض آخر،
+
+125
+00:09:53,540 --> 00:09:57,710
+تمام؟ ممكن تكون أحد أعراض مرض معين، أو ممكن تكون مرض
+
+126
+00:09:57,710 --> 00:10:02,570
+بحد ذاتها، فبالتالي إذا لما بيكون عندي الخلايا إمّا
+
+127
+00:10:02,570 --> 00:10:07,610
+صغيرة أو قليلة أو ما بتحملش أكسجين بشكل كافي، ممكن
+
+128
+00:10:07,610 --> 00:10:13,190
+هذا يشكّل جهدًا على الـ heart و الـ lung، must work
+
+129
+00:10:13,190 --> 00:10:17,010
+harder، تمام؟ إذا في جهد عليهم لحتى يعوّضوا الـ
+
+130
+00:10:17,010 --> 00:10:23,750
+oxygen اللي بيتم فقده أو افتقاده في الجسم، anemia
+
+131
+00:10:23,750 --> 00:10:27,370
+is caused by many different diseases or disorders،
+
+132
+00:10:27,370 --> 00:10:30,690
+حكينا هذه المعلومة، حكينا إن الأنيميا ممكن تكون كـ
+
+133
+00:10:30,690 --> 00:10:37,030
+disease منفصل بحد ذاته، أو ممكن تكون يعني عرض من
+
+134
+00:10:37,030 --> 00:10:40,770
+أعراض disease أو disorder آخر، the first step in
+
+135
+00:10:40,770 --> 00:10:44,230
+finding the cause is to determine what type of anemia
+
+136
+00:10:44,230 --> 00:10:50,210
+طبعًا، حتى أنا أول خطوة بشخص فيها الأنيميا، بدي أعرف
+
+137
+00:10:50,550 --> 00:10:55,430
+وصف هاي الأنيميا، تمام؟ يعني هي anemia تسببت في
+
+138
+00:10:55,430 --> 00:11:02,490
+قلة في الـ RBC فقط، أو anemia تسببت بصغر في حجم الـ
+
+139
+00:11:02,490 --> 00:11:06,990
+RBC، أو قلة في تركيز الـ hemoglobin، هذه كلها مين
+
+140
+00:11:06,990 --> 00:11:10,110
+اللي هيدلّني عليها؟ مين اللي هيصف لي هاي النوع الـ
+
+141
+00:11:10,110 --> 00:11:14,550
+anemia؟ الـ red blood cell indices هي اللي هتساعدني
+
+142
+00:11:14,550 --> 00:11:20,910
+to classify the anemia، لحتى أصنف الأنيميا، مع خلايا
+
+143
+00:11:20,910 --> 00:11:24,970
+صغيرة الحجم، أنيميا مع خلايا، تركيز الـ hemoglobin
+
+144
+00:11:24,970 --> 00:11:29,110
+قليل فيها، أنيميا، تركيز الـ hemoglobin طبيعي وحجمها
+
+145
+00:11:29,110 --> 00:11:33,890
+طبيعي، بس عم تتكسر بسرعة، فبالتالي في عندي فقط few
+
+146
+00:11:33,890 --> 00:11:39,700
+number فيها، ولا شو بالضبط، تمام؟ فإذا الإجابة راح
+
+147
+00:11:39,700 --> 00:11:44,940
+تكون نوع الأنيميا هاي أو الوصف لهذه الأنيميا عند
+
+148
+00:11:44,940 --> 00:11:49,460
+الـ red blood cell indices، مؤشرات خلايا الدم
+
+149
+00:11:49,460 --> 00:11:54,950
+الحمراء، نبدأ مع أول معامل أو مؤشر اللي هو الـ mean
+
+150
+00:11:54,950 --> 00:11:59,950
+corpuscular volume، حكينا متوسط حجم الكرية أو
+
+151
+00:11:59,950 --> 00:12:04,650
+الخلية، تمام؟ اللي هو الـ MCV، تعريفه is the average
+
+152
+00:12:04,650 --> 00:12:10,870
+volume of red blood cells، طبعًا تركيزه، بيتم قياس التركيز
+
+153
+00:12:10,870 --> 00:12:15,650
+أو وحدة القياس هي الفيمتولتر، تمام؟ الفيمتولتر و
+
+154
+00:12:15,650 --> 00:12:19,910
+الفيمتولتر هو عبارة عن عشرة أس سالب خمسة عشر من الـ
+
+155
+00:12:19,910 --> 00:12:26,460
+liter، طبعًا، طيب الـ MCV هو عبارة عن إيش؟ طيب خلينا
+
+156
+00:12:26,460 --> 00:12:30,820
+بس نستنتج القوانين هذه، الـ mathematical formulas،
+
+157
+00:12:30,820 --> 00:12:36,400
+القوانين الرياضية هاي بشكل منطقي جدًا، اتعرفنا على الـ
+
+158
+00:12:36,400 --> 00:12:41,340
+hematocrit، وحكينا هو الحجم أو الحيز اللي بيشغله...
+
+159
+00:12:41,340 --> 00:12:46,540
+تشغله الـ RBCs كلها بالنسبة للعينة، تمام؟ وإحنا لما
+
+160
+00:12:46,540 --> 00:12:50,480
+كنا بنحسب الـ hematocrit كنا بنفترض إن العينة كلها
+
+161
+00:12:50,480 --> 00:12:56,300
+100% وبنشوف كم نسبة بتشغله الـ RBCs من هذه العينة
+
+162
+00:12:56,300 --> 00:12:59,820
+ككل، تمام؟ طيب يعني إذا الـ hematocrit هو حجم الـ
+
+163
+00:12:59,820 --> 00:13:04,160
+RBCs ككل، كل الـ RBCs، هيك بنصفّها على بعض وبنشوف
+
+164
+00:13:04,160 --> 00:13:09,360
+حجمها، تمام؟ بالمئة، طيب الـ RBCs count، عدد خلايا
+
+165
+00:13:09,360 --> 00:13:15,220
+الدم الحمراء، طيب لو أنا... طب لو قسمت الحجم اللي
+
+166
+00:13:15,220 --> 00:13:21,520
+بتشغله الـ RBCs كلها على عددها ككل، مش بالنهاية بحصل
+
+167
+00:13:21,520 --> 00:13:26,700
+على الحجم اللي بتحتله الـ RBCs الواحدة، فبالنهاية أنا
+
+168
+00:13:26,700 --> 00:13:32,380
+بحصل على الـ average volume of RBCs، يعني متوسط لحجم
+
+169
+00:13:32,380 --> 00:13:36,860
+خلية واحدة من الـ RBCs، تمام؟ إذا بقسم الحجم اللي
+
+170
+00:13:36,860 --> 00:13:41,960
+بتحتله الـ RBCs كلها على عدد هاي الـ RBCs، وبالنهاية
+
+171
+00:13:41,960 --> 00:13:46,320
+بحصل على متوسط حجم الخلية، طبعًا بضرب الشيء هذا
+
+172
+00:13:46,320 --> 00:13:53,530
+بعشرة عشان في النهاية أنا أحصل على يعني ال... الرقم
+
+173
+00:13:53,530 --> 00:13:58,110
+يكون منطقي فقط، طيب إذا ال... ال... الـ hematocrit
+
+174
+00:13:58,
+
+223
+00:17:58,510 --> 00:18:04,520
+normal إذا الخلل إما هيكون في ال life span، في عمر
+
+224
+00:18:04,520 --> 00:18:07,400
+الخلية أو ممكن يكون في ال hemoglobin content،
+
+225
+00:18:07,400 --> 00:18:15,620
+تمام؟ طيب microcytic anemia إذا هي أنيميا نتجت عن
+
+226
+00:18:15,620 --> 00:18:19,640
+أن الخلايا small cells يعني في عندها decreased
+
+227
+00:18:19,640 --> 00:18:24,820
+MCV تمام أما ال macrocytic anemia فهي عبارة عن
+
+228
+00:18:24,820 --> 00:18:29,980
+أنيميا نتجت عن خلايا large cells كبيرة يعني
+
+229
+00:18:29,980 --> 00:18:35,520
+increased MCV طيب نتسأل هل الخلية ال RBCs لما تكون
+
+230
+00:18:35,520 --> 00:18:39,690
+كبيرة ممكن ينتج أنيميا؟ بتكون كبيرة وفاضية على
+
+231
+00:18:39,690 --> 00:18:42,670
+الفاضي يعني حجم تمام فبالتالي .. يعني مافيش فيها
+
+232
+00:18:42,670 --> 00:18:47,150
+hemoglobin كافي فبالتالي بتنتج لي أنيميا منها تمام
+
+233
+00:18:47,150 --> 00:18:50,590
+طب إيش أمثلة على ال microcytic anemia ال
+
+234
+00:18:50,590 --> 00:18:54,830
+microcytic anemia هي مثال عليها ال iron deficiency
+
+235
+00:18:54,830 --> 00:18:59,290
+أنيميا و الثلاسيميا تمام ال iron deficiency أنيميا
+
+236
+00:18:59,290 --> 00:19:03,490
+اللي هي أنيميا نقص الحديد و الثلاسيميا اللي هي ال
+
+237
+00:19:03,490 --> 00:19:09,830
+…  أنيميا البحر الأبيض المتوسط هدول الإثنين بنسميهم
+
+238
+00:19:09,830 --> 00:19:13,890
+microcytic الخلايا بتكون فيها صغيرة و ال
+
+239
+00:19:13,890 --> 00:19:17,330
+hemoglobin content فيها قليل تمام ال hemoglobin
+
+240
+00:19:17,330 --> 00:19:22,960
+فيها قليل ليش؟ لأن هنا ما عنديش iron كفاية فال… ال
+
+241
+00:19:22,960 --> 00:19:26,400
+hemoglobin اللي بيتصنع ما بيكونش… ما إله iron
+
+242
+00:19:26,400 --> 00:19:32,140
+فبالتالي بيكون فيه نقص في ال… ال iron فبت… في
+
+243
+00:19:32,140 --> 00:19:36,760
+ال hemoglobin كمان و نقص في ال oxygen delivery
+
+244
+00:19:36,760 --> 00:19:42,060
+الثلاسيميا هي طفرة في جينات ال… ال hemoglobin
+
+245
+00:19:42,060 --> 00:19:46,880
+فبيتم تصنيع… بيصير فيه decrease production من ال
+
+246
+00:19:46,880 --> 00:19:52,610
+hemoglobin أو ممكن يكون الطفرة أعطتني هيموغلوبين
+
+247
+00:19:52,610 --> 00:19:55,890
+شكله و تركيبه abnormal فبالتالي
+
+248
+00:19:55,890 --> 00:20:00,570
+وظيفته هتكون abnormal ما بتحمل يعني أكسجين
+
+249
+00:20:00,570 --> 00:20:04,930
+طيب ال macrocytic anemia أو ال macrocytic cells
+
+250
+00:20:04,930 --> 00:20:09,570
+طيب أنيميا نتجت عن خلايا كبيرة في الحجم هذه
+
+251
+00:20:09,570 --> 00:20:14,030
+بنشوفها في liver disease وفي ال hypothyroidism تمام
+
+252
+00:20:14,030 --> 00:20:19,650
+يعني خلايا الغدة الدرقية هرموناتها
+
+253
+00:20:19,650 --> 00:20:23,390
+قليلة بتنتج هرمونات قليلة هي معناه hypo قليل
+
+254
+00:20:23,390 --> 00:20:28,870
+thyroidism يعني هرمونات الغدة الدرقية تمام ال liver
+
+255
+00:20:28,870 --> 00:20:33,590
+disease مشكلة في الكبد ممكن ينتج عنها أن الخلايا
+
+256
+00:20:33,590 --> 00:20:39,410
+بتكون macrocytic كبيرة طيب ممكن لما يكون في عندي
+
+257
+00:20:39,410 --> 00:20:43,890
+asynchronous in RBCs maturation يعني مافيش تناسق في
+
+258
+00:20:43,890 --> 00:20:48,470
+نضوج ال RBCs تمام؟ و هذا بنسميه megaloblastic
+
+259
+00:20:48,470 --> 00:20:52,230
+anemia و هذه ال megaloblastic anemia يعني خلايا
+
+260
+00:20:52,230 --> 00:20:56,230
+كبيرة تمام؟ و لكن في عندي anemia يعني مافيش
+
+261
+00:20:56,230 --> 00:21:01,330
+هيموغلوبين أو يعني أنه مافيش RBCs كافية تمام؟
+
+262
+00:21:01,330 --> 00:21:05,190
+فبالتالي هذه بتنتج عن ال folate عن ال folic acid و
+
+263
+00:21:05,190 --> 00:21:08,930
+ال vitamin B12 deficiencies طبعا خلينا بس نحكي
+
+264
+00:21:08,930 --> 00:21:13,370
+حدوطة صغيرة عشان نعرف إيش يعني أنه مافيش تناسق
+
+265
+00:21:13,370 --> 00:21:19,730
+هلق ال RBCs أثناء نضوجها في ال bone marrow بتمر
+
+266
+00:21:19,730 --> 00:21:23,450
+بمراحل بتكون عندي خلية أم إحنا متفقين أنه في
+
+267
+00:21:23,450 --> 00:21:27,890
+عندي خلية أم لكل نوع من أنواع ال blood cells فعندي
+
+268
+00:21:27,890 --> 00:21:33,260
+في عندي خلية أم ل ال RBCs هذه الخلية الأم مجرد ما
+
+269
+00:21:33,260 --> 00:21:39,760
+الجسم يحتاج RBCs بيرسل لها رسالة فبتنشط و بتبدأ
+
+270
+00:21:39,760 --> 00:21:44,100
+تنقسم و تنضج لحتى تعطيني RBCs و ال RBCs هادي
+
+271
+00:21:44,100 --> 00:21:47,120
+بتخرج و بتطلع من ال… من ال bone marrow و بتطلع ع ال
+
+272
+00:21:47,120 --> 00:21:53,060
+circulation تمام؟ طيب… هلق أثناء نضوج الخلايا
+
+273
+00:21:53,060 --> 00:21:58,630
+هاي… اللي خلينا نحكي عنها ال progenitors اللي
+
+274
+00:21:58,630 --> 00:22:04,370
+هي يعني الخلايا السلالة الخلايا الأصل ل RBCs
+
+275
+00:22:04,370 --> 00:22:11,490
+تمام؟ أثناء نضوجها بتكون لها نواة يعني لما بتصل
+
+276
+00:22:11,490 --> 00:22:15,150
+لآخر مراحل في النضوج بتفقد النواة هذه عشان هيك ال
+
+277
+00:22:15,150 --> 00:22:21,310
+RBCs بتطلع على ال… ال circulation تمام؟ بتطلع
+
+278
+00:22:21,310 --> 00:22:30,420
+فاقدة نواتها مافيش فيها نواة تمام؟ و ال… أثناء
+
+279
+00:22:30,420 --> 00:22:36,420
+أيضا نضوجها الخلية بيصغر حجمها، تمام؟ بتكون كبيرة،
+
+280
+00:22:36,420 --> 00:22:39,560
+الخلايا ال mother، الأم في المراحل الأولى من
+
+281
+00:22:39,560 --> 00:22:43,540
+النضوج بتكون كبيرة، بتبدأ تصغر، تصغر، تصغر، بعدين
+
+282
+00:22:43,540 --> 00:22:48,740
+بتفقد النواة، بعدين بتطلع على ال circulation إذن في
+
+283
+00:22:48,740 --> 00:22:53,060
+عندي أحداث بت… بتصير أثناء ال maturation اللي هي
+
+284
+00:22:53,060 --> 00:22:56,820
+أول حاجة صغر في الحجم تدريجي لحد ما تصل لعندي ال
+
+285
+00:22:56,820 --> 00:23:00,860
+MCV ال normal اللي هي من 80 ل 100 و بعدين بتطلع ال
+
+286
+00:23:00,860 --> 00:23:09,270
+circulation و أيضا… بيكون في فقدان للنواة و أيضا
+
+287
+00:23:09,270 --> 00:23:15,470
+بيكون في عندي ال hemoglobin… تصنيع لل
+
+288
+00:23:15,470 --> 00:23:19,170
+hemoglobin، تمام؟ hemoglobin synthesis فال
+
+289
+00:23:19,170 --> 00:23:23,210
+hemoglobin synthesis هذا ما بيضل شغال على طول، لأ
+
+290
+00:23:23,980 --> 00:23:27,480
+الخلية لما بتطلع من ال… ال… ال bone marrow
+
+291
+00:23:27,480 --> 00:23:32,120
+لازم تكون وصلت لتركيز معين من ال hemoglobin
+
+292
+00:23:32,120 --> 00:23:35,760
+ك… كافٍ أنه يخرجها من ال bone marrow و تروح ع
+
+293
+00:23:35,760 --> 00:23:42,560
+ال circulation، طبعا، إحنا هنا طلاب مثلا بتدرسوا،
+
+294
+00:23:42,560 --> 00:23:47,560
+بتنضجوا، بتتعلموا، بتكتسبوا خبرات، تمام؟ بتجتازوا
+
+295
+00:23:47,560 --> 00:23:52,480
+امتحانات و لما نصل لعند مرحلة معينة من العلم عندكم
+
+296
+00:23:52,480 --> 00:23:56,660
+كافية أننا نخرجكم، تتخرجوا، تمام؟ نفس الشيء ال
+
+297
+00:23:56,660 --> 00:24:05,460
+RBCs بتنضج، بتنقسم و بتفقد نواتها، بتصنع
+
+298
+00:24:05,460 --> 00:24:09,220
+هيموغلوبين، الهيموغلوبين هذا بيصل لمستوى كافٍ إنها
+
+299
+00:24:09,220 --> 00:24:14,040
+تطلع من ال bone marrow فتطلع، تمام؟ طيب لو كان في
+
+300
+00:24:14,040 --> 00:24:18,560
+عندي نقص في ال… ال iron طبعا، لو كان في عندي نقص
+
+301
+00:24:18,560 --> 00:24:21,580
+في ال iron أو حتى نقص في تصنيع ال hemoglobin ذات
+
+302
+00:24:21,580 --> 00:24:26,880
+نفسه، الخلية بتضلها تنقسم، تنقسم، تنقسم ل… لحد
+
+303
+00:24:26,880 --> 00:24:30,240
+… يعني بدها تصل لمرحلة أنه يكون ال hemoglobin
+
+304
+00:24:30,240 --> 00:24:35,020
+اللي موجود فيها كافٍ إنها تتخرج، طبعا؟ كافٍ إنها
+
+305
+00:24:35,020 --> 00:24:39,500
+تطلع من ال bone marrow طبعا فبتضطر إنها تبضل تنقسم
+
+306
+00:24:39,500 --> 00:24:45,220
+تنقسم لحد ما تصل لحجم كثير صغير عليها أنه يكون ال
+
+307
+00:24:45,220 --> 00:24:49,220
+hemoglobin اللي فيها كافٍ إنها تطلع عشان يطلعها ال
+
+308
+00:24:49,220 --> 00:24:55,610
+bone marrow طبعا فعشان هيك في حالة نقصان ال iron و
+
+309
+00:24:55,610 --> 00:24:59,150
+في حال تخلل في تصنيع ال hemoglobin أو نقصان في
+
+310
+00:24:59,150 --> 00:25:02,630
+تصنيع ال hemoglobin تمام مافيش عندي hemoglobin
+
+311
+00:25:02,630 --> 00:25:05,470
+كافٍ في ال bone marrow… في ال… ال RBCs في ال
+
+312
+00:25:05,470 --> 00:25:10,090
+bone marrow تمام فبالتالي ال RBCs إيش اللي عمل
+
+313
+00:25:10,090 --> 00:25:15,330
+أمامها أن هتظلها تنقسم تمام؟ انقسامات وانقسامات
+
+314
+00:25:15,330 --> 00:25:19,670
+وانقسامات لحد ما تصل لحجم كثير صغير ما هي كل ما
+
+315
+00:25:19,670 --> 00:25:24,250
+بتنقسم كل ما بيصغر حجمها تمام؟ لحد ما تصل لحجم كثير
+
+316
+00:25:24,250 --> 00:25:28,350
+صغير و يكون شوية ال hemoglobin اللي فيها كافية
+
+317
+00:25:28,350 --> 00:25:32,170
+عليها بالنسبة لحجمها طبعا كافية إنها تتخرج و تطلع
+
+318
+00:25:32,170 --> 00:25:36,530
+من ال bone marrow تمام؟ فعشان هيك ال iron
+
+319
+00:25:36,530 --> 00:25:41,880
+deficiency و الثلاسيميا بينتج عنها microcytic
+
+320
+00:25:41,880 --> 00:25:47,320
+cells خلية ال MCV تبعها قليل عرفنا ليش بسبب
+
+321
+00:25:47,320 --> 00:25:52,680
+الانقسامات الكثيرة لحتى تصل لل target تبعها اللي
+
+322
+00:25:52,680 --> 00:25:55,880
+هو ال hemoglobin content اللي لازم تكون على الأقل
+
+323
+00:25:55,880 --> 00:26:02,400
+محتوية بالنسبة لحجمها طيب في حالة ال folic acid
+
+324
+00:26:02,400 --> 00:26:07,480
+deficiency و ال vitamin B12 deficiency إيش بيصير؟ طبعا
+
+325
+00:26:07,480 --> 00:26:13,600
+هدول يا بنات بيلزموا لانقسام النواة تمام؟ بيلزموا
+
+326
+00:26:13,600 --> 00:26:18,360
+الخلية أثناء انقسام نواتها طبعا طيب هدول تم
+
+327
+00:26:18,360 --> 00:26:23,380
+نقسانهن إذا النواة مش راح تنقسم إذا الخلية راح
+
+328
+00:26:23,380 --> 00:26:29,250
+تظلها كبيرة وإحنا حكينا كل ما تنقسم كل ما تصغر في
+
+329
+00:26:29,250 --> 00:26:36,130
+الحجم طيب في عندي عوامل أصلا يعني متطلبات و مهمة
+
+330
+00:26:36,130 --> 00:26:39,690
+جدا لانقسام النواة و في عندي deficiencies فيهم، في
+
+331
+00:26:39,690 --> 00:26:44,810
+نقصان فيهم، تمام؟ طيب فبالتالي النواة مش راح تنقسم
+
+332
+00:26:44,810 --> 00:26:49,870
+فالخلية ممكن تفقد… يعني راح تبقى تصنع hemoglobin
+
+333
+00:26:49,870 --> 00:26:54,250
+و تصل ال target بس لسه حجمها كبير فبتفقد النواة
+
+334
+00:26:54,250 --> 00:27:00,450
+early تمام؟ يتم التخلص من نواتها و هي لسه حجمها
+
+335
+00:27:00,450 --> 00:27:06,600
+كبير تمام؟ هذا بنسميه asynchronous عدم تناسق ما
+
+336
+00:27:06,600 --> 00:27:10,420
+بين انقسام النواة و نضوج ال… أو ال… ال
+
+337
+00:27:10,420 --> 00:27:13,180
+cytoplasm يعني أنه يعني أنه يكون فيه محتوى
+
+338
+00:27:13,180 --> 00:27:18,780
+هيموغلوبين كافٍ تمام؟ فبينتج عنه إما microcytic أو
+
+339
+00:27:18,780 --> 00:27:23,000
+macrocytic cells تمام؟ هذا ال asynchronous بينتج
+
+340
+00:27:23,000 --> 00:27:29,800
+عنه microcytic أو macrocytic إذا تأخر تصنيع ال
+
+341
+00:27:29,800 --> 00:27:35,380
+hemoglobin الكافٍ لتخريج ال RBCs من ال bone marrow
+
+342
+00:27:35,380 --> 00:27:41,140
+تستمر الخلية في الانقسام لحد ما تصل ل microcytic
+
+343
+00:27:41,140 --> 00:27:48,380
+تمام؟ و إذا تأخر نضوج النواة و انقسام النواة تستمر
+
+344
+00:27:48,380 --> 00:27:54,280
+الخلية بتصنيع ال hemoglobin ولكن بعدها بتضطر…
+
+345
+00:27:54,280 --> 00:27:57,280
+بتصل… لأ خلاص بتصل لل target اللي كافٍ إنه
+
+346
+00:27:57,280 --> 00:28:00,920
+يخرجها من ال bone marrow فبتضطر إنها تتخلص من
+
+347
+00:28:00,920 --> 00:28:04,480
+النواة و هي لساتها حجمها كبير لأنها ما انقسمت
+
+348
+00:28:04,480 --> 00:28:09,020
+كفاية لأن العوامل اللي بتلزمها لانقسام نواتها أصلا
+
+349
+00:28:09,020 --> 00:28:13,620
+فيها deficiency فالخلية بتطلع لي macrocytic تمام؟
+
+350
+00:28:13,620 --> 00:28:19,980
+بتطلع macrocytic كبيرة طيب ال mean corpuscular
+
+351
+00:28:19,980 --> 00:28:25,880
+hemoglobin متوسط ال hemoglobin في الكرية طبعا؟ طب
+
+352
+00:28:25,880 --> 00:28:29,940
+متوسط ال hemoglobin إيش؟ وزن ال hemoglobin طبعا؟
+
+353
+00:28:29,940 --> 00:28:34,480
+the average weight of hemoglobin in an RBCs طيب
+
+354
+00:28:34,480 --> 00:28:39,320
+وحدة القياس ال picogram طبعا؟ ال picogram و ال
+
+355
+00:28:39,320 --> 00:28:43,620
+picogram هو عبارة عن عشرة أس سالب اثنا عشر ملي جرام طيب
+
+356
+00:28:44,640 --> 00:28:50,320
+خلينا نشوف طيب ك… يعني كتحليل منطقي لقينا
+
+357
+00:28:50,320 --> 00:28:58,700
+القانون نشوف إيش يعني MCH ال MCH إحنا حكينا متوسط
+
+358
+00:28:58,700 --> 00:29:05,200
+وزن ال hemoglobin في ال… ال RBCs الواحدة تمام؟
+
+359
+00:29:05,200 --> 00:29:11,280
+طيب إحنا لما كنا نقيس ال hemoglobin… sorry لما
+
+360
+00:29:11,280 --> 00:29:14,720
+كنا نقيس ال hemoglobin إيش كنا نحكي؟ كنا نحكي ال
+
+361
+00:29:14,720 --> 00:29:19,540
+total hemoglobin إيش يعني؟ يعني ال hemoglobin اللي
+
+362
+00:29:19,540 --> 00:29:24,620
+في كل خلايا الدم per microliter صح؟ تمام يعني أنه
+
+363
+00:29:24,620 --> 00:29:29,060
+أنا بالنهاية كنت لما أحصل على ال hemoglobin كتركيز
+
+364
+00:29:29,060 --> 00:29:33,920
+كنت أحصل عليه بوحدة gram per deciliter تمام؟ عدد
+
+365
+00:29:33,920 --> 00:29:39,380
+الخلايا بال deciliter تمام؟ كلها هذه أنا أخذت ال
+
+366
+00:29:39,380 --> 00:29:46,700
+hemoglobin اللي فيها و طلعت وزنه طب أنا لو عارفة
+
+367
+00:29:46,700 --> 00:29:53,560
+الخلايا هذه عددها ككل مش ممكن يعني أقسم وزن ال…
+
+368
+00:29:53,560 --> 00:29:57,960
+ال… ال hemoglobin ككل على عدد الخلايا ككل
+
+369
+00:29:57,960 --> 00:30:03,770
+بيطلع لي وزن ال hemoglobin في الخلية الواحدة فإذا
+
+370
+00:30:03,770 --> 00:30:07,690
+هذا هو تفسير القانون اللي هو hemoglobin ككل بال gram
+
+371
+00:30:07,690 --> 00:30:13,630
+per deciliter على ال RBCs count ككل و ضرب عشرة
+
+372
+00:30:13,630 --> 00:30:19,610
+طبعا فال normal range بيطلع من 28 ل 32 picogram
+
+373
+00:30:20,640 --> 00:30:26,180
+طبعا ال MCH نادرا أو يعني مش عادة بيستخدم لتصنيف
+
+374
+00:30:26,180 --> 00:30:30,540
+ال anemia ولكنه أحيانا بيكون مؤشر جيد عن محتوى ال
+
+375
+00:30:30,540 --> 00:30:36,080
+hemoglobin في داخل ال RBCs كوزن تمام؟ هاي هنا مثال
+
+376
+00:30:36,080 --> 00:30:41,840
+كتطبيق للقانون ال hemoglobin 16 ال RBCs 5 مليون
+
+377
+00:30:41,840 --> 00:30:47,270
+طبعا أنا بأخذ كم مليون؟ 5 خلاص بعوض في الخمسة طيب
+
+378
+00:30:47,270 --> 00:30:51,330
+16 على 5 ده 3.2 بيطلع عندي طبعا 16 على 5 ثلاثة
+
+379
+00:30:51,330 --> 00:30:55,870
+point 2 ال… في عشرة 32 picogram
+
+380
+00:30:55,870 --> 00:31:01,070
+تمام؟ و نفس الشيء إذا كان ال… ال… ال MCH
+
+381
+00:31:01,070 --> 00:31:08,510
+أقل من 28 it is low و إذا كان أكثر من 32 it's high
+
+382
+00:31:08,510 --> 00:31:14,150
+طيب؟ و لكن إحنا حكينا مش دائما أنا بستخدمه في
+
+383
+00:31:14,150 --> 00:31:17,090
+classification للأنيميا في تصنيف الأنيميا أو في
+
+384
+00:31:17,090 --> 00:31:21,750
+وصف الأنيميا طيب ال mean corpuscular hemoglobin
+
+385
+00:31:21,750 --> 00:31:27,630
+concentration اللي هو ال MCHC هو عبارة عن متوسط
+
+386
+00:31:27,630 --> 00:31:32,030
+تمام ال hemoglobin concentration متوسط تركيز ال
+
+387
+00:31:32,030 --> 00:31:35,150
+hemoglobin في الكرية الواحدة أو في الخلية الواحدة
+
+388
+00:31:35,460 --> 00:31:38,860
+فإذن هو عبارة عن the average concentration متوسط
+
+389
+00:31:38,860 --> 00:31:43,420
+التركيز ل hemoglobin في كل خلية من خلايا ال… ال
+
+390
+00:31:43,420 --> 00:31:48,500
+… ال RBCs تمام؟ في الخلية الواحدة طيب ف… ال
+
+391
+00:31:48,500 --> 00:31:53,480
+… وحدة القياس طبعا بتكون as a percentage أو ممكن
+
+392
+00:31:53,480 --> 00:31:57,840
+أن أنا أحكي عنها gram per deciliter تمام؟ طيب
+
+393
+00:31:59,450 --> 00:32:03,910
+طبعا ال… ال MCHC قانونه ال hemoglobin على ال
+
+394
+00:32:03,910 --> 00:32:08,810
+hematocrit ضرب 100% تمام؟ تركيز ال hemoglobin ككل
+
+395
+00:32:08,810 --> 00:32:13,150
+على
+
+445
+00:35:49,280 --> 00:35:55,200
+blood film حكينا إن الـ RBCs بتكون طبعا فيها منطقة
+
+446
+00:35:55,200 --> 00:35:59,400
+تلتها في المركز بيكون فيه central pallor شحوب مركزي
+
+447
+00:35:59,400 --> 00:36:04,580
+منطقة خالية من الـ hemoglobin لو كانت الخلية طبعا
+
+448
+00:36:04,580 --> 00:36:07,620
+ما .. لا تحتوي على central pallor إذا هي
+
+449
+00:36:07,620 --> 00:36:13,120
+spherocytic تمام؟ وإذا هي hyperchromic تجاوزا بصفها
+
+450
+00:36:13,120 --> 00:36:17,220
+كـ hyperchromic لأنها it's as big as full تظهر
+
+451
+00:36:17,220 --> 00:36:21,020
+كأنها مليانة هموجلوبين ولكن فعليا لا يتجاوز الـ 37
+
+452
+00:36:21,020 --> 00:36:27,560
+gram per deciliter طبعا فإذا حسبنا MCHC أعلى من 37
+
+453
+00:36:27,560 --> 00:36:31,700
+gram per deciliter فهذا معناها أنه الهموجلوبين
+
+454
+00:36:31,700 --> 00:36:37,020
+value تركيز الهموجلوبين هنا عالي .. الهموجلوبين عالي
+
+455
+00:36:37,020 --> 00:36:40,940
+طبعا فيه error عالي البسط عالي يعني الـ MCHC هيطلع
+
+456
+00:36:40,940 --> 00:36:47,100
+عالي طبعا فمعناها فيه عندي error في الهموجلوبين
+
+457
+00:36:47,100 --> 00:36:55,480
+value طيب إذا MCV normal MCHC normal طبعا يعني
+
+458
+00:36:55,480 --> 00:37:07,190
+normal إذا هي normocytic normal normal normal
+
+459
+00:37:07,190 --> 00:37:07,250
+normal normal normal normal normal normal normal
+
+460
+00:37:07,250 --> 00:37:07,830
+normal normal normal normal normal normal normal
+
+461
+00:37:07,830 --> 00:37:08,110
+normal normal normal normal normal normal normal
+
+462
+00:37:08,110 --> 00:37:08,430
+normal normal normal normal normal normal normal
+
+463
+00:37:08,430 --> 00:37:10,370
+normal normal normal normal normal normal normal
+
+464
+00:37:10,370 --> 00:37:18,630
+normal normal normal normal normal
+
+465
+00:37:18,630 --> 00:37:18,670
+normal normal normal normal normal normal normal
+
+466
+00:37:18,670 --> 00:37:18,750
+normal normal normal normal normal normal normal
+
+467
+00:37:18,750 --> 00:37:18,790
+normal normal normal normal normal normal normal
+
+468
+00:37:18,790 --> 00:37:21,710
+normal normal normal normal normal طيب لو كان فيه
+
+469
+00:37:21,710 --> 00:37:24,930
+عندي decrease في الإثنين، إيش بدي أوصف الـ anemia؟
+
+470
+00:37:24,930 --> 00:37:28,390
+بدي أحكي عن إنه قلة في الـ MCV أو قلة في الحجم أو
+
+471
+00:37:28,390 --> 00:37:35,310
+صغر في الحجم يعني decrease MCV microcytic حكينا ب
+
+472
+00:37:35,310 --> 00:37:41,000
+.. بأعبر عنها كأنها microcytic بأستخدم هذا اللفظ طيب
+
+473
+00:37:41,000 --> 00:37:45,220
+قلة في اللون أو يعني hemoglobin concentration قليل
+
+474
+00:37:45,220 --> 00:37:48,740
+أيضا decrease طبعا دي يعني decrease إذا
+
+475
+00:37:48,740 --> 00:37:52,900
+hypochromic حكينا إذا بسميها microcytic
+
+476
+00:37:52,900 --> 00:37:56,460
+hypochromic anemia ومثال على الـ microcytic
+
+477
+00:37:56,460 --> 00:37:59,820
+hypochromic anemia اللي هي إما iron deficiency
+
+478
+00:37:59,820 --> 00:38:05,420
+anemia أو ثلاسيميا طبعا طيب macrocytic
+
+479
+00:38:06,390 --> 00:38:12,250
+يعني إيش؟ الـ MCV عالي increase تمام؟ و
+
+480
+00:38:12,250 --> 00:38:18,470
+normochromic يعني الـ MCHC normal تمام؟ فهذا معنى
+
+481
+00:38:18,470 --> 00:38:21,470
+أنه الخلية أو الأنيميا عندي macrocytic
+
+482
+00:38:21,470 --> 00:38:26,670
+normochromic طبعا فيه عندي أسماء من الـ .. الـ .. أو
+
+483
+00:38:26,670 --> 00:38:32,110
+أنواع من الأنيميات اللي بيندرجوا تحت هاي الـ ..
+
+484
+00:38:32,110 --> 00:38:39,140
+الوصف تحت هدول الأوصاف تمام؟ فبالتالي الخطوة الأولى
+
+485
+00:38:39,140 --> 00:38:49,140
+إن أنا أقيم شكل الـ .. الـ .. الـ RBCs وأشوف مؤشرات
+
+486
+00:38:49,140 --> 00:38:55,940
+الـ RBCs هذه تمام؟ أصف نوع الأنيميا فبالتالي
+
+487
+00:38:55,940 --> 00:38:59,660
+بتحصر لي أنواع معينة مثلا normocytic-normochromic
+
+488
+00:38:59,660 --> 00:39:03,520
+فيه عندي أنواع معينة واحد اثنين ثلاثة فبضل .. خلاص
+
+489
+00:39:03,520 --> 00:39:07,800
+بنحصرها بهذول الأنواع ببدأ أعمل فحوصات ثانية تفرق
+
+490
+00:39:07,800 --> 00:39:10,860
+ما بين الأنواع تبع normocytic-normochromic
+
+491
+00:39:10,860 --> 00:39:18,640
+تمام؟ كانت مؤشرات وشكل الـ .. الـ RBCs والـ indices
+
+492
+00:39:18,640 --> 00:39:22,640
+اللي أنا حسبتهم بيحكوا لي أنها microcytic
+
+493
+00:39:22,640 --> 00:39:25,860
+hypochromic إذا أنا حتوجه إما حتكون ثلاسيميا أو
+
+494
+00:39:25,860 --> 00:39:30,200
+iron deficiency anemia ممكن أعمل فحوصات لمستوى الـ
+
+495
+00:39:30,200 --> 00:39:34,160
+iron ممكن يعني أعمل فحوصات لحتى أكشف عن جينات الـ
+
+496
+00:39:34,160 --> 00:39:37,400
+hemoglobin وأشوف إذا فيها خلل عشان أشخص ثلاسيميا
+
+497
+00:39:37,400 --> 00:39:43,320
+يعني فبالتالي أكون محصورة بالأنواع اللي هي
+
+498
+00:39:43,320 --> 00:39:46,820
+microcytic hypochromic تمام؟ macrocytic
+
+499
+00:39:46,820 --> 00:39:50,480
+-normochromic برضه كمان نفس الأشياء فبالتالي إذا
+
+500
+00:39:50,480 --> 00:39:54,720
+حكينا إنها بتساعدني في وصف نوع الأنيميا والـ
+
+501
+00:39:54,720 --> 00:39:57,840
+classification والـ differential diagnosis التفريق
+
+502
+00:39:57,840 --> 00:40:06,790
+ما بين هاي الأنيميات تمام؟ طيب هاي مصطلحات راح أن
+
+503
+00:40:06,790 --> 00:40:12,590
+نذكرها already صرنا بنعرفها بس خلوني بس أحكي
+
+504
+00:40:12,590 --> 00:40:20,880
+نقطتين أول شيء الـ .. بنهاية المحاضرة هاي إحنا
+
+505
+00:40:20,880 --> 00:40:27,400
+تقريبا صرنا بنعرف نشتغل وبنعرف نحسب كل بنود الـ
+
+506
+00:40:27,400 --> 00:40:31,160
+CBC اللي هو الـ complete blood count تمام؟ الـ
+
+507
+00:40:31,160 --> 00:40:36,680
+complete blood count بيكون فيه عد لـ الـ WBCs و
+
+508
+00:40:36,680 --> 00:40:41,060
+بيكون فيه عد لـ الـ RBCs وإحنا إتعرفنا عليهم وإتعرفنا
+
+509
+00:40:41,060 --> 00:40:48,500
+كيف بنشتغلهم وكيف بنحسبهم manual تمام؟ وأيضا
+
+510
+00:40:48,500 --> 00:40:53,200
+بيكون فيه differential لـ الـ white cells يعني كام
+
+511
+00:40:53,200 --> 00:40:55,960
+نسبة الـ monocyte، كام نسبة الـ lymphocyte، الـ
+
+512
+00:40:55,960 --> 00:40:59,420
+neutrophil، الـ eosinophil، الـ basophil، تمام،
+
+513
+00:40:59,420 --> 00:41:04,280
+فبرضه هذه أنا أعطيتكم فكرة عنها أنه إحنا من خلال
+
+514
+00:41:04,280 --> 00:41:09,280
+blood film بنحسب، يعني بنعيد 100 خلية وبنشوف كام
+
+515
+00:41:09,280 --> 00:41:11,680
+طلع معنا منهم الـ neutrophil، كام طلع معنا منهم
+
+516
+00:41:11,680 --> 00:41:16,670
+monocyte، وكام lymphocyte للآخرين وبنحسب نسبة
+
+517
+00:41:16,670 --> 00:41:21,970
+المئوية لهم تمام طيب وأيضا بيكون فيه حساب للـ
+
+518
+00:41:21,970 --> 00:41:25,010
+hemoglobin concentration وصرنا بنعرف بطريقة
+
+519
+00:41:25,010 --> 00:41:30,790
+Sahli method بنعرف نحسب تركيز الـ hemoglobin برضه
+
+520
+00:41:30,790 --> 00:41:36,830
+manual يدويا وبكون فيه حساب لتركيز أو عدد الـ
+
+521
+00:41:36,830 --> 00:41:40,390
+platelets الصفائح الدموية من خلال blood film يمكن
+
+522
+00:41:40,390 --> 00:41:46,390
+عدها أيضا فإذا إحنا صرنا بنعرف تقريبا نعمل CBC
+
+523
+00:41:46,390 --> 00:41:51,070
+يدوي صح؟ وبس مسألة الـ differential يعني لو عديت الـ
+
+524
+00:41:51,070 --> 00:41:54,850
+platelets حتأخذوها أكثر بشكل موسع في الـ hematology
+
+525
+00:41:56,980 --> 00:42:02,440
+اللي بيفرق أنه الـ CBC بيكون automated جهاز بيشتغله
+
+526
+00:42:02,440 --> 00:42:05,820
+تمام؟ ولكن إحنا بنفرق عن الجهاز يعني أنا في
+
+527
+00:42:05,820 --> 00:42:09,340
+النهاية ممكن الجهاز يغلط لكن أنا لازم أكون فاهمة و
+
+528
+00:42:09,340 --> 00:42:13,080
+عارفة المبدأ أو من وين اجت الحسابات وكيف الجهاز
+
+529
+00:42:13,080 --> 00:42:16,380
+أصلا بيعد وكيف بيشتغل فدي كلها لازم نكون إحنا
+
+530
+00:42:16,380 --> 00:42:19,940
+بنعرفها عشان هيك إحنا إتعلمنا الطريقة اليدوية
+
+531
+00:42:19,940 --> 00:42:24,520
+الجهاز بيستخدم المعادلات هاي لحتى يحسب الـ indices
+
+532
+00:42:24,520 --> 00:42:29,060
+وبيطلع لي MCV وMCH وMCHC وهيك أنتم إتعلمتوا
+
+533
+00:42:29,060 --> 00:42:34,820
+كمان اليوم إن كيف تحسبوهم فبالتالي إذا يعني صار فيه
+
+534
+00:42:34,820 --> 00:42:38,780
+عندنا رؤية واضحة أنا كيف ممكن أعمل complete blood
+
+535
+00:42:38,780 --> 00:42:41,000
+count لشخص يدويا
+
+536
+00:42:43,450 --> 00:42:47,050
+طيب خلينا نشوف إيش يعني anemia مرت معنا أنه هي
+
+537
+00:42:47,050 --> 00:42:50,490
+عبارة عن conditions، ظروف، variety of conditions
+
+538
+00:42:50,490 --> 00:42:55,750
+طبعا، متنوعة هذه الظروف المحصلة إن الـ person's
+
+539
+00:42:55,750 --> 00:42:59,350
+blood دم الشخص can't carry as much oxygen as it
+
+540
+00:42:59,350 --> 00:43:03,870
+should يعني ما بيحملش oxygen كما ينبغي اللي إما
+
+541
+00:43:03,870 --> 00:43:08,330
+بيكون سبب أنه الـ number أو الـ size أو الـ
+
+542
+00:43:08,330 --> 00:43:15,130
+hemoglobin content للـ RBCs قليل طبعا والحالة يعني
+
+543
+00:43:15,130 --> 00:43:21,150
+بيكون المحصلة hypoxia تمام hypochromic حكينا hypo
+
+544
+00:43:21,150 --> 00:43:26,470
+نقصان أو قلة في الـ color اللون يعني قلة في تركيز الـ
+
+545
+00:43:26,470 --> 00:43:31,490
+hemoglobin إذا هو المصطلح اللي بيطبق على الـ RBCs
+
+546
+00:43:31,490 --> 00:43:34,490
+لما بتكون decreased concentration of hemoglobin
+
+547
+00:43:34,490 --> 00:43:39,090
+عندها نقص في تركيز الـ hemoglobin طبعا macrocytic
+
+548
+00:43:39,090 --> 00:43:43,330
+يعني خلية macro كبيرة، cytic خلية كبيرة، إذا
+
+549
+00:43:43,330 --> 00:43:47,370
+larger than normal red blood cell, mean corpuscular
+
+550
+00:43:47,370 --> 00:43:51,590
+hemoglobin هو الـ average hemoglobin طبعا weight,
+
+551
+00:43:51,910 --> 00:43:55,270
+وزن الـ hemoglobin في الـ RBCs, mean corpuscular
+
+552
+00:43:55,270 --> 00:43:57,150
+hemoglobin concentration هو الـ average
+
+553
+00:43:57,150 --> 00:44:01,110
+concentrationللهموجلوبين في الـ red blood cell الـ
+
+554
+00:44:01,110 --> 00:44:03,990
+mean corpuscular volume هو الـ average volume للـ red
+
+555
+00:44:03,990 --> 00:44:09,190
+blood cell طبعا micro صغيرة، cytic خلية إذا خلية
+
+556
+00:44:09,190 --> 00:44:12,910
+smaller than normal الـ MCV تبعها حيكون قليل
+
+557
+00:44:12,910 --> 00:44:16,970
+normochromic خلية اللون فيها طبيعي إذا الـ red
+
+558
+00:44:16,970 --> 00:44:19,490
+blood cell عندها normal concentration of
+
+559
+00:44:19,490 --> 00:44:24,310
+hemoglobin normocytic يعني خلية حجمها طبيعي إذا الـ
+
+560
+00:44:24,310 --> 00:44:28,390
+red blood cell normal size الـ MCV normal والـ red
+
+561
+00:44:28,390 --> 00:44:32,930
+blood cell indices كشكل يعني كشكل عام إنها عبارة
+
+562
+00:44:32,930 --> 00:44:36,890
+عن المؤشرات والقياسات اللي بتفسر الحجم والـ
+
+563
+00:44:36,890 --> 00:44:40,030
+hemoglobin content للـ red blood cell تمام؟ وهذا
+
+564
+00:44:40,030 --> 00:44:44,010
+هو التعريف اللي إحنا بدأنا في محاضرتنا عم نختم فيه
+
+565
+00:44:44,010 --> 00:44:48,350
+هلق وهيك بنكون وصلنا لنهاية المحاضرة يعطيكم ألف
+
+566
+00:44:48,350 --> 00:44:48,770
+عافية
diff --git a/PL9fwy3NUQKwY9jl8xw-bWkVZkWcGGyspd/Up-Q0W10hks_postprocess.srt b/PL9fwy3NUQKwY9jl8xw-bWkVZkWcGGyspd/Up-Q0W10hks_postprocess.srt
new file mode 100644
index 0000000000000000000000000000000000000000..02602559b07ee20d4b0bf3de1e467daba703ef09
--- /dev/null
+++ b/PL9fwy3NUQKwY9jl8xw-bWkVZkWcGGyspd/Up-Q0W10hks_postprocess.srt
@@ -0,0 +1,2264 @@
+1
+00:00:10,020 --> 00:00:13,720
+السلام عليكم ورحمة الله وبركاته طالبات العزيزات
+
+2
+00:00:13,720 --> 00:00:17,940
+اليوم ان شاء الله راح نتحدث عن red blood cell
+
+3
+00:00:17,940 --> 00:00:23,000
+indices مؤشرات خلايا الدم الحمراء أو معاملات خلايا
+
+4
+00:00:23,000 --> 00:00:26,760
+الدم الحمراء في البداية خلينا نعرف شو يعني red
+
+5
+00:00:26,760 --> 00:00:30,560
+blood cell indicesRed blood cell indices هي عبارة
+
+6
+00:00:30,560 --> 00:00:35,120
+عن measurement قياسات مؤشرات أو معاملات that
+
+7
+00:00:35,120 --> 00:00:39,840
+describe the size and oxygen carrying protein
+
+8
+00:00:39,840 --> 00:00:44,700
+content of red blood cell إذن هي معاملات ومؤشرات
+
+9
+00:00:44,700 --> 00:00:50,480
+بتصف حجم خلية ال RBCs بالإضافة إلى محتوها من ال
+
+10
+00:00:50,480 --> 00:00:53,620
+homoglobin اللي هو عبارة عن ال oxygen carrying
+
+11
+00:00:53,620 --> 00:00:57,240
+protein البروتين المسئول عن حملة ال oxygen ونقلة
+
+12
+00:00:57,740 --> 00:01:02,600
+طمام؟ إذا red blood cell indices هي مؤشرات تصف حجم
+
+13
+00:01:02,600 --> 00:01:07,400
+الخلية ومحتوها من ال homoglobin وإذا بتتذكروا لما
+
+14
+00:01:07,400 --> 00:01:11,960
+شرحنا محاضرة ال blood film حكينا إنه من خلال ال
+
+15
+00:01:11,960 --> 00:01:14,640
+blood film أو ال blood smear أنا بقيم ال
+
+16
+00:01:14,640 --> 00:01:19,220
+morphology ال shape للخليةوحكيتلكم بالنسبة لل RBCs
+
+17
+00:01:19,220 --> 00:01:24,200
+أنا بدرس .. بشوف حجمها بدرس أيضا محتوها من ال
+
+18
+00:01:24,200 --> 00:01:27,280
+homoglobin بشوفها خلايا فاضية أو فيها homoglobin
+
+19
+00:01:27,280 --> 00:01:30,600
+ال central baller اللي .. اللي هو شحوب المركز اللي
+
+20
+00:01:30,600 --> 00:01:35,100
+في داخلها هل يحتل طولتها او اكثر من ذلك وكل هذه
+
+21
+00:01:35,100 --> 00:01:40,640
+لها مؤشرات و مرتبطة بتشخيص ال anemia تمام؟ اذا ال
+
+22
+00:01:40,640 --> 00:01:44,440
+red blood cell indices ايضا هي عبارة عن عوامل
+
+23
+00:01:44,440 --> 00:01:51,600
+ومؤشراتيعني حسابات قياسات أيضا بتدلليلي عن حجم
+
+24
+00:01:51,600 --> 00:01:56,480
+الخلية ومحتوها من ال homoglobin تمام؟ وبالتالي إذا
+
+25
+00:01:56,480 --> 00:02:01,660
+تستخدم بالإضافة لل blood film بتستخدم to help in
+
+26
+00:02:01,660 --> 00:02:05,820
+the differential diagnosis of anemia إذا تساعدني
+
+27
+00:02:05,820 --> 00:02:10,420
+لحتى أفرق ما بين أنواع الأنيميا و أشخص نوع
+
+28
+00:02:10,420 --> 00:02:15,980
+الأنيميا الصحيح أو الدقيق تمام؟The relationships
+
+29
+00:02:15,980 --> 00:02:20,700
+between hematocrit, homoglobin level and RBCs
+
+30
+00:02:20,700 --> 00:02:25,020
+count are converted to blood cell indices through
+
+31
+00:02:25,020 --> 00:02:29,320
+mathematical formulas تمام؟ احنا حكينا أنا بقيم
+
+32
+00:02:29,320 --> 00:02:33,360
+حجم الخليةمحتوها من الـ Homoglobin بالـ blood film
+
+33
+00:02:33,360 --> 00:02:36,820
+عن طريق الـ morphology الشكلها طبعا أنا بحفظ شكلها
+
+34
+00:02:36,820 --> 00:02:41,820
+خلية RBCs تلتينها Homoglobin تلتها Central Baller
+
+35
+00:02:41,820 --> 00:02:46,680
+و بيصير حجمها مقلوف لإلي طبعا بالنسبة لل Red blood
+
+36
+00:02:46,680 --> 00:02:50,000
+cell indices كيف أنا بقيم ال size و ال Homoglobin
+
+37
+00:02:50,000 --> 00:02:54,630
+content من خلالحسابات من خلال mathematical formula
+
+38
+00:02:54,630 --> 00:03:00,510
+صياغ رياضية تمام استخدم فيها أو تم استخدام فيها ال
+
+39
+00:03:00,510 --> 00:03:04,850
+hematocrit و ال hemoglobin level و ال RBCs إذا تم
+
+40
+00:03:04,850 --> 00:03:10,390
+توظيف ال hematocrit ال hemoglobin level و أيضا ال
+
+41
+00:03:10,390 --> 00:03:16,910
+RBCs count لحتىاللي بتم صياغة معادلات حسابية بتأشر
+
+42
+00:03:16,910 --> 00:03:21,930
+أو بتدل أو بتعتبر كمعاملات تعبر عن حجم الخلية
+
+43
+00:03:21,930 --> 00:03:27,690
+ومحتوها من ال homoglobin تمام؟ إذا لنرتب أفكارنا
+
+44
+00:03:27,690 --> 00:03:31,770
+red blood cell indices هي عبارة عن قياسات أو
+
+45
+00:03:31,770 --> 00:03:36,890
+مؤشرات أو معاملات تعبر عن حجم الخلية ومحتوها من ال
+
+46
+00:03:36,890 --> 00:03:40,050
+homoglobin بتفيدني لحتى أعمل differential
+
+47
+00:03:40,050 --> 00:03:45,840
+diagnosis للأنمياتمام؟ وكيف انا بحصل على هاي
+
+48
+00:03:45,840 --> 00:03:49,960
+القياسات او هاي المؤشرات تم توظيف ال hemoglobin و
+
+49
+00:03:49,960 --> 00:03:53,300
+ال level او ال hemoglobin concentration و ال
+
+50
+00:03:53,300 --> 00:03:58,440
+hematocrit و ال RBC count من خلال صيغ .. صيغ
+
+51
+00:03:58,440 --> 00:04:04,820
+رياضيةمن خلال معرفة العلاقة ما بين هاي ال .. ال ..
+
+52
+00:04:04,820 --> 00:04:08,340
+المكونات أو هاي الحسابات الهيموتوكرت وال
+
+53
+00:04:08,340 --> 00:04:13,080
+hemoglobin level و ال RBC count طم صياغة ال .. ال
+
+54
+00:04:13,080 --> 00:04:16,300
+indices هاي طب شو هي ال indices هاي؟ المؤشرات هاي
+
+55
+00:04:16,300 --> 00:04:20,320
+عبارة عن إيه؟ هي تلت مؤشرات ال mean corpuscular
+
+56
+00:04:20,320 --> 00:04:26,190
+volumeو ال mcv و ال mean corpuscular homoglobin و
+
+57
+00:04:26,190 --> 00:04:30,210
+ال mean corpuscular homoglobin concentration mcv
+
+58
+00:04:30,210 --> 00:04:36,630
+mch mchc طبعا طيب ال mean متوسط معناها corpuscular
+
+59
+00:04:36,630 --> 00:04:42,450
+يعني يعني بتعود على كلمة كرة لإن احنا بنحكي عن
+
+60
+00:04:42,450 --> 00:04:46,790
+خلايا الدم الحمراء كريات الدم الحمراء و كريات الدم
+
+61
+00:04:46,790 --> 00:04:50,470
+البيضاء طبعا فإذا corpuscular يعني جاية من .. من
+
+62
+00:04:50,470 --> 00:04:54,750
+.. من ..من شيء كراوي أي يعبّر عن الخلية، تمام؟
+
+63
+00:04:54,750 --> 00:04:58,510
+يعني هو متوسط، اذا mean carboscular volume هاي ..
+
+64
+00:04:58,510 --> 00:05:03,090
+هاي Syria معناها متوسط حجم الخلية، mean
+
+65
+00:05:03,090 --> 00:05:07,570
+carboscular homoglobin متوسط حجم ال .. او متوسط
+
+66
+00:05:07,570 --> 00:05:12,050
+سوري ال homoglobin content ل الخلية او الكريه
+
+67
+00:05:12,050 --> 00:05:15,130
+تمام؟ و ال mean carboscular homoglobin
+
+68
+00:05:15,130 --> 00:05:19,330
+concentration متوسط تركيز ال homoglobin في الكريه
+
+69
+00:05:19,330 --> 00:05:26,640
+او في الخليةتمام؟ اذا MCV, MCH, MCHC هدول هم ال
+
+70
+00:05:26,640 --> 00:05:31,060
+indices هدول هم المؤشرات و القياسات اللي لهم صياغ
+
+71
+00:05:31,060 --> 00:05:35,740
+و معادلات حسابية باستخدام ال hemotocrit و ال
+
+72
+00:05:35,740 --> 00:05:40,140
+homoglobin level و RBC count انا ممكن احسبهم تمام؟
+
+73
+00:05:40,140 --> 00:05:43,920
+و بيدللولي على حجم الخلية و على محتوها من ال
+
+74
+00:05:43,920 --> 00:05:49,210
+homoglobin و هذا بساعدني بتشخيص القنينة تمام؟نيجي
+
+75
+00:05:49,210 --> 00:05:54,450
+لال bare bones الغرض من هاي ال indices في البداية
+
+76
+00:05:54,450 --> 00:05:59,630
+نعرف إيش يعني anemia anemia include a variety of
+
+77
+00:05:59,630 --> 00:06:04,610
+conditions with the same outcome يعني ال .. ال
+
+78
+00:06:04,610 --> 00:06:11,330
+anemia ممكن يكون أسبابها عديدة ممكن يكون سببها إما
+
+79
+00:06:11,330 --> 00:06:18,130
+نقص في تصنيع ال homoglobin نقص في ال ironالخلل في
+
+80
+00:06:18,130 --> 00:06:23,810
+تصنيع ال homoglobin حتى كمان ممكن يكون shortage أو
+
+81
+00:06:23,810 --> 00:06:29,450
+حتى ال life span يعني قصر في عمر الخلية تمام ال
+
+82
+00:06:29,450 --> 00:06:35,170
+life span قليلة ل ال RBCs ممكن يكون
+
+83
+00:06:38,790 --> 00:06:45,690
+الخلية تفتقد عنصر ال folic acid أو ال vitamin B12
+
+84
+00:06:45,690 --> 00:06:49,550
+اللي بيلزمها لحتى تنقسم داخل البون مارو وتنضج
+
+85
+00:06:49,550 --> 00:06:54,410
+وتخرج إلى ال circulation تمام؟ إذا ممكن إن ال RBC
+
+86
+00:06:54,410 --> 00:06:58,550
+بتتصنع طبيعي عددها طبيعي و ال homoglobin تباعها
+
+87
+00:06:58,550 --> 00:07:03,560
+طبيعي بس لما بتطلع علىال .. ال circulation بتتكسر
+
+88
+00:07:03,560 --> 00:07:10,380
+بسرعة، إذا عندي أسباب عديدة لإن .. نحكي عنها إنها
+
+89
+00:07:10,380 --> 00:07:14,220
+بتسببلي أنميا، تمام؟ إذا إن هي عبارة عن variety of
+
+90
+00:07:14,220 --> 00:07:19,340
+conditions، في ظروف عديدة ممكن تسببلي أنميا ولكن
+
+91
+00:07:19,340 --> 00:07:24,840
+المحصلة كلها يتم وصفها بإنه ال blood can't carry
+
+92
+00:07:24,840 --> 00:07:29,350
+as much oxygen as it shouldال .. ال .. ال blood
+
+93
+00:07:29,350 --> 00:07:33,890
+كمية الدم و كمية ال RBCs اللي موجودة لا تحمل
+
+94
+00:07:33,890 --> 00:07:39,130
+أكسوجين كافي إلى ال tissues تمام كما ينبغي as it
+
+95
+00:07:39,130 --> 00:07:44,390
+should فبالتالي الشخص بيبدأ يعاني من hypoxia إيش
+
+96
+00:07:44,390 --> 00:07:49,040
+يعني hypoxia؟ يعني نقص أكسوجين في الجسمالسبب إنه
+
+97
+00:07:49,040 --> 00:07:52,840
+إما ال RBCs عمرها قصير أو بتتكسر بسرعة أو بيتم
+
+98
+00:07:52,840 --> 00:07:58,320
+تصنيعها بشكل مش كافي ال homoglobin بيتصنع بشكل
+
+99
+00:07:58,320 --> 00:08:02,420
+قليل أو يعني بتركيز قليل أو حتى كمان بيتصنعوا في
+
+100
+00:08:02,420 --> 00:08:06,640
+خطأ في تصنيعه مافيش iron كفاية لل homoglobin لإنه
+
+101
+00:08:06,640 --> 00:08:09,620
+حكينا ال iron أساسي لتركيب ال homoglobin اللي هو
+
+102
+00:08:09,620 --> 00:08:13,600
+أصلا أساسي لحمل ال oxygenتمام؟ الخلايا و هي عم
+
+103
+00:08:13,600 --> 00:08:18,120
+تنقسم صار فيها خلال معين هذه كلها conditions تمام؟
+
+104
+00:08:18,120 --> 00:08:22,680
+المحصلة hypoxia اذا و بيصير ان ال blood can't
+
+105
+00:08:22,680 --> 00:08:28,000
+carry oxygen as much as it should تمام؟أما ال
+
+106
+00:08:28,000 --> 00:08:31,660
+healthy person الشخص الطبيعي ال normal has
+
+107
+00:08:31,660 --> 00:08:35,260
+adequate number of correctly sized red blood cell
+
+108
+00:08:35,260 --> 00:08:38,700
+that contain enough hemoglobin to carry sufficient
+
+109
+00:08:38,700 --> 00:08:44,150
+oxygen at all the body's tissuesتمام الشخص الطبيعي
+
+110
+00:08:44,150 --> 00:08:48,830
+عنده adequate number، عدد طبيعي وكافي من ال RBCs،
+
+111
+00:08:48,830 --> 00:08:52,610
+اللي حجمها طبيعي و اللي محتوها من ال homoglobin
+
+112
+00:08:52,610 --> 00:08:56,850
+أيضا كافي فبالتالي هي بتحمل oxygen sufficient،
+
+113
+00:08:56,850 --> 00:09:02,490
+كافي لل all body tissues، لكل أنسجة الجسمتمام an
+
+114
+00:09:02,490 --> 00:09:06,970
+animic person has red blood cells that are either
+
+115
+00:09:06,970 --> 00:09:11,190
+too small or too few in number الشخص اللي .. اللي
+
+116
+00:09:11,190 --> 00:09:15,270
+.. اللي بنحكي عنه animic عنده أنيميا ال .. ال red
+
+117
+00:09:15,270 --> 00:09:18,510
+blood cell تبعته ممكن تكون صغير، حجمها صغير كتير
+
+118
+00:09:18,510 --> 00:09:21,630
+فبالتالي محتوىها من ال homoglobin هيكون قليل أو
+
+119
+00:09:21,630 --> 00:09:28,250
+ممكن يكون عددها قليلطبعا إما نتيجة ضعف الإنتاج أو
+
+120
+00:09:28,250 --> 00:09:32,050
+حتى ممكن لإنها ال life span حكينا فيها shortage
+
+121
+00:09:32,050 --> 00:09:35,550
+قصيرة المتوسط عمر الخلية تطلع ع ال circulation
+
+122
+00:09:35,550 --> 00:09:41,860
+تتكسر بسرعة طبعا بيصيرلها licenseف whatever السبب
+
+123
+00:09:41,860 --> 00:09:46,980
+في النهاية هذا كله بينوصف ك anemia ال anemia ممكن
+
+124
+00:09:46,980 --> 00:09:53,540
+تكون كمرض منفصل بحد ذاته او ممكن تكون عرض لمرض اخر
+
+125
+00:09:53,540 --> 00:09:57,710
+تمام؟ممكن تكون احد اعراض مرض معين او ممكن تكون مرض
+
+126
+00:09:57,710 --> 00:10:02,570
+بحد ذاتها فبالتالي اذا لما بيكون عندى الخلايا اما
+
+127
+00:10:02,570 --> 00:10:07,610
+صغيرة او قليلة او مابتحملش اكسوجين بشكل كافي ممكن
+
+128
+00:10:07,610 --> 00:10:13,190
+هذا يشكل جهد على ال heart و ال lung must work
+
+129
+00:10:13,190 --> 00:10:17,010
+harder تمام؟ اذا فى جهد عليهم لحتى يعوضوا ال
+
+130
+00:10:17,010 --> 00:10:23,750
+oxygen اللى بيتم فقدانه او افتقاده في الجسمanemia
+
+131
+00:10:23,750 --> 00:10:27,370
+is caused by many different diseases or disorders
+
+132
+00:10:27,370 --> 00:10:30,690
+حكينا هذه المعلومة حكينا ان الانيميا ممكن تكون ك
+
+133
+00:10:30,690 --> 00:10:37,030
+disease منفصل بحد ذاته او ممكن تكون يعني عرض من
+
+134
+00:10:37,030 --> 00:10:40,770
+اعراض disease او disorder اخر the first step in
+
+135
+00:10:40,770 --> 00:10:44,230
+finding the cause is determine what type of anemia
+
+136
+00:10:44,230 --> 00:10:50,210
+طبعا حتى انا اول خطوة بشخص فيها الانيميا بدي اعرف
+
+137
+00:10:50,550 --> 00:10:55,430
+وصف هاي ال anemia تمام؟ يعني هى anemia تسببت في
+
+138
+00:10:55,430 --> 00:11:02,490
+قلة فى ال RBC فقط او anemia تسببت بصغر فى حجم ال
+
+139
+00:11:02,490 --> 00:11:06,990
+RBC او قلة فى تركيز ال homoglobin هدى كلها مين
+
+140
+00:11:06,990 --> 00:11:10,110
+اللى هيدلللي عليها؟ مين اللى هيصفلي هاى النوع ال
+
+141
+00:11:10,110 --> 00:11:14,550
+anemia ال red blood cell indices هى اللى هتساعدني
+
+142
+00:11:14,550 --> 00:11:20,910
+to classify the anemia لحتى أصنف ال anemiaمع خلايا
+
+143
+00:11:20,910 --> 00:11:24,970
+صغيرة الحجم أنينيا مع خلايا تركيز ال homoglobin
+
+144
+00:11:24,970 --> 00:11:29,110
+قليل فيها أنينيا تركيز ال homoglobin طبيعي و حجمها
+
+145
+00:11:29,110 --> 00:11:33,890
+طبيعي بس عم تتكسر بسرعة فبالتالي في عندى فقط few
+
+146
+00:11:33,890 --> 00:11:39,700
+number فيها ولا شو بالضبطتمام؟ فإذا الإجابة راح
+
+147
+00:11:39,700 --> 00:11:44,940
+تكون نوع الأنيميا هاي أو الوصف لهذه الأنيميا عند
+
+148
+00:11:44,940 --> 00:11:49,460
+ال red blood cell indices مؤشرات خلايا الدم
+
+149
+00:11:49,460 --> 00:11:54,950
+الحمراءنبدأ مع أول معامل أو مؤشر اللي هو ال mean
+
+150
+00:11:54,950 --> 00:11:59,950
+carboscular volume حكينا متوسط حجم الكورية أو
+
+151
+00:11:59,950 --> 00:12:04,650
+الخلية تمام اللي هو ال MCV تعريفه is the average
+
+152
+00:12:04,650 --> 00:12:10,870
+volume of herbicides طبعا تركيزه بيتم قياس التركيز
+
+153
+00:12:10,870 --> 00:12:15,650
+أو وحدة القياس هي الفيمتو لتر تمام الفيمتو لتر و
+
+154
+00:12:15,650 --> 00:12:19,910
+الفيمتو لتر هو عبارة عن عشرة أسالب خمستاش من ال
+
+155
+00:12:19,910 --> 00:12:26,460
+letterطبعا طيب ال MCV هو عبارة عن إيش؟ طيب خلينا
+
+156
+00:12:26,460 --> 00:12:30,820
+بس نستنتج القوانين هذه ال mathematical formulas
+
+157
+00:12:30,820 --> 00:12:36,400
+القوانين الرياضية هاي بشكل منطقي جدااتعرفنا على ال
+
+158
+00:12:36,400 --> 00:12:41,340
+hematocrit وحكينا هو الحجم أو الحيز اللي بيشغل ..
+
+159
+00:12:41,340 --> 00:12:46,540
+تشغل ال RBCs كلها بالنسبة للعينة تمام؟ وإحنا لما
+
+160
+00:12:46,540 --> 00:12:50,480
+كنا بنحسب ال hematocrit كنا بنفترض إن العينة كلها
+
+161
+00:12:50,480 --> 00:12:56,300
+100% و بنشوف كام نسبة بتشغل ال RBCs من هذه العينة
+
+162
+00:12:56,300 --> 00:12:59,820
+ككلتمام؟ طيب يعني إذا ال hematocrite هو حجم ال
+
+163
+00:12:59,820 --> 00:13:04,160
+RBCs ككل كل ال RBCs هيك بنصفطها على بعض و بنشوف
+
+164
+00:13:04,160 --> 00:13:09,360
+حجمها تمام؟ بالمية طيب ال RBCs count عدد خلايا
+
+165
+00:13:09,360 --> 00:13:15,220
+الدم الحمراء طيب لو أنا .. طب لو قسمت الحجم اللي
+
+166
+00:13:15,220 --> 00:13:21,520
+بتشغله ال RBCs كلها على عددها ككل مش بالنهاية بحصل
+
+167
+00:13:21,520 --> 00:13:26,700
+على الحجم اللي بتحتله ال RBCs الواحدةفبالنهاية انا
+
+168
+00:13:26,700 --> 00:13:32,380
+بحصل على ال average volume of RBCs يعني متوسط لحجم
+
+169
+00:13:32,380 --> 00:13:36,860
+خلية واحدة من ال RBCs تمام؟ اذا بقسم الحجم اللي
+
+170
+00:13:36,860 --> 00:13:41,960
+بتحتله ال RBCs كلها على عدد هاي ال RBCs و بالنهاية
+
+171
+00:13:41,960 --> 00:13:46,320
+بحصل على متوسط حجم الخلية طبعا بدرب الاشي هذا
+
+172
+00:13:46,320 --> 00:13:53,530
+بعشرة عشان في النهاية انا احصل على يعني ال ..الرقم
+
+173
+00:13:53,530 --> 00:13:58,110
+يكون منطقي فقط طيب إذا ال .. ال .. ال hemotocrit
+
+174
+00:13:58,110 --> 00:14:00,830
+هاي هنا مثال بيعطيني hemotocrit خمسة و أربعين في
+
+175
+00:14:00,830 --> 00:14:07,670
+المية و ال RBC's خمسة مليون per liter طيب ال MCV
+
+176
+00:14:07,670 --> 00:14:11,910
+هي عبارة عن إيش؟ هي عبارة عن الخمسة و أربعين على
+
+177
+00:14:11,910 --> 00:14:15,230
+مين؟ على ال millions فقط يعني على فقط العدد هذا
+
+178
+00:14:15,230 --> 00:14:18,750
+أنا مابستخدم العشرة أقصد اتناش في الحسابات طيب هو
+
+179
+00:14:18,750 --> 00:14:23,670
+صح خمسة مليونسل بير مايكرو ليتر تمام؟ هي أقصى
+
+180
+00:14:23,670 --> 00:14:27,570
+اتناش عشان bear microliter .. bear liter هنا sorry
+
+181
+00:14:27,570 --> 00:14:31,410
+هي عشرة أقصى اتناش عشانها bear liter لكن لو كانت
+
+182
+00:14:31,410 --> 00:14:36,560
+مثلا bear microliter هتكون عشرة أقصى ستةطيب ف ..
+
+183
+00:14:36,560 --> 00:14:39,880
+في ال .. ما .. سواء كانت per liter أو per
+
+184
+00:14:39,880 --> 00:14:43,720
+microliter الرقم اللي هنا اللي هو عشر أقص أنا
+
+185
+00:14:43,720 --> 00:14:47,940
+مابدياه، تمام؟ أنا فقط بدي .. كام مليون؟ هم خمسة
+
+186
+00:14:47,940 --> 00:14:51,500
+مليون، خلاص أنا باخد الخمسة، تمام؟ اذا هنا ال
+
+187
+00:14:51,500 --> 00:14:55,740
+RBC's count أنا فقط بدي عدد المليونات، كام مليون؟
+
+188
+00:14:55,740 --> 00:15:01,920
+خمسة مليون، خلاص خمسة فقططمام؟ ف45 على 5 فبيطلع 9
+
+189
+00:15:01,920 --> 00:15:06,140
+ضرب 10 اذا 90 fem to liter هو الحجم طيب ال normal
+
+190
+00:15:06,140 --> 00:15:11,020
+range ل MCV بيقع ما بين 80 إلى 100 اذا ال MCV
+
+191
+00:15:11,020 --> 00:15:16,710
+normal ما بين 80 إلى 100طيب هذه الخلية الطبيعية
+
+192
+00:15:16,710 --> 00:15:21,750
+اللي يعني حجمها بيتراوح ما بين 80 femtoliter إلى
+
+193
+00:15:21,750 --> 00:15:27,390
+100 femtoliter طبعا و ممكن يكون 85 ممكن يكون 90
+
+194
+00:15:27,390 --> 00:15:32,250
+اذا it's as a range طبعا طيب لو .. لو افترض انه
+
+195
+00:15:32,250 --> 00:15:40,640
+طلع عندي الخلية حجمهاأقل من 80 تمام؟ إذا كان أقل
+
+196
+00:15:40,640 --> 00:15:46,540
+من 80 معناها خلية صغيرة إذا هذا ال normal range و
+
+197
+00:15:46,540 --> 00:15:49,880
+كانت الخلية أقل من 80 يعني أقل من ال lower limit
+
+198
+00:15:49,880 --> 00:15:54,260
+لل range معناها الخلية صغيرة في الحجم طيب إيش
+
+199
+00:15:54,260 --> 00:15:58,220
+المصطلح اللي بيصف إن الخلية تكون صغيرة ال smaller
+
+200
+00:15:58,220 --> 00:16:02,420
+cell إن هي تكون microcytic إذا لما أحكي عن خلاء
+
+201
+00:16:02,420 --> 00:16:07,290
+خلية صغيرة في الحجمبحكي عنها إن هي micro يعني
+
+202
+00:16:07,290 --> 00:16:11,210
+صغيرة micro طبعا citic .. citic جاية من كلمة site
+
+203
+00:16:11,210 --> 00:16:17,450
+أو خلية طبعا micro citic خلية صغيرة طيب لو كان ال
+
+204
+00:16:17,450 --> 00:16:22,910
+MCV أكتر من مية more than one hundred فهمته later
+
+205
+00:16:23,190 --> 00:16:28,010
+طيب هتنحكي عنها larger cell اللي هي macrocytic
+
+206
+00:16:28,010 --> 00:16:32,590
+طبعا إذا micro يعني صغير، macro كبير، cytic خلية
+
+207
+00:16:32,590 --> 00:16:38,130
+microcytic خلية صغيرة و macrocytic خلية كبيرة و
+
+208
+00:16:38,130 --> 00:16:41,610
+هلأ استنا بنعرف إيش يعني خلية صغيرة و إيش يعني
+
+209
+00:16:41,610 --> 00:16:47,410
+خلية كبيرة microcytic يعني ال MCV تبعها أقل من 80و
+
+210
+00:16:47,410 --> 00:16:53,670
+macrocytic ال MCV خلية ال .. ال MCV أكثر من مائة
+
+211
+00:16:53,670 --> 00:16:59,330
+تمام؟ ال microcytic خلية ال MCV أقل من تمانين و ال
+
+212
+00:16:59,330 --> 00:17:07,010
+macrocytic خلية ال MCV أكثر من مائة طيب إذا هنا بس
+
+213
+00:17:07,010 --> 00:17:14,080
+املخصلي إنه normocyticعفوان ال normocytic هي عبارة
+
+214
+00:17:14,080 --> 00:17:18,600
+عن يعني أو وصفلي normocytic anemia إيش يعني؟ يعني
+
+215
+00:17:18,600 --> 00:17:24,620
+خلية ال .. ال RBCs فيها normal size normocytic
+
+216
+00:17:24,620 --> 00:17:31,160
+anemia يعني خلية ال .. ال MCV normal عندها، تمام؟
+
+217
+00:17:31,160 --> 00:17:37,030
+معناهاالخلل اللي سبب لي أنيميا مش في حجم ال .. ال
+
+218
+00:17:37,030 --> 00:17:41,290
+RBCs طبعا احنا هنا حكينا ان ال RBCs .. ان الانيميا
+
+219
+00:17:41,290 --> 00:17:44,750
+ممكن تنتج عن ال small او ال RBCs تكون if you ال
+
+220
+00:17:44,750 --> 00:17:48,490
+number طبعا و ممكن تكون ال .. ال low concentration
+
+221
+00:17:48,490 --> 00:17:52,930
+of homoglobin طبعا فإذا لما نحكي عن normostatic
+
+222
+00:17:52,930 --> 00:17:58,510
+أنيميا معناها هي عبارة عن أنيميا الخلية حجمها
+
+223
+00:17:58,510 --> 00:18:04,520
+normalإذا الخلل إما هيكون في ال life span، في عمر
+
+224
+00:18:04,520 --> 00:18:07,400
+الخلية أو ممكن يكون في ال homoglobin content،
+
+225
+00:18:07,400 --> 00:18:15,620
+تمام؟طيب microcytic anemia إذا هي أنيمية نتجت عن
+
+226
+00:18:15,620 --> 00:18:19,640
+إنه الخلايا small cells يعني في عندها decreased
+
+227
+00:18:19,640 --> 00:18:24,820
+MCV تمام أما ال macrocytic anemia فهي عبارة عن
+
+228
+00:18:24,820 --> 00:18:29,980
+أنيمية نتجت عن خلايا large cell كبيرة يعني
+
+229
+00:18:29,980 --> 00:18:35,520
+increased MCV طيب نتسأل هل الخلية ال RBCs لما تكون
+
+230
+00:18:35,520 --> 00:18:39,690
+كبيرة ممكن ينتج أنيمية؟بتكون كبيرة وفاضية على
+
+231
+00:18:39,690 --> 00:18:42,670
+الفاضي يعني حجم تمام فبالتالي .. يعني مافيش فيها
+
+232
+00:18:42,670 --> 00:18:47,150
+homoglobin كافي فبالتالي بتنتجلي أنيميا منها تمام
+
+233
+00:18:47,150 --> 00:18:50,590
+طب إيش أمثلة على ال microcytic anemia ال
+
+234
+00:18:50,590 --> 00:18:54,830
+microcytic anemia هي مثال عليها ال iron deficiency
+
+235
+00:18:54,830 --> 00:18:59,290
+أنيميا و السلسيميا تمام ال iron deficiency أنيميا
+
+236
+00:18:59,290 --> 00:19:03,490
+اللي هي أنيميا نقص الحديد و السلسيميا اللي هي ال
+
+237
+00:19:03,490 --> 00:19:09,830
+..Anemia البحر الأبيض المتوسط هدول التنتين بنسميهم
+
+238
+00:19:09,830 --> 00:19:13,890
+microcytic الخلايا بتكون فيها صغيرة و ال
+
+239
+00:19:13,890 --> 00:19:17,330
+homoglobin content فيها قليل تمام ال homoglobin
+
+240
+00:19:17,330 --> 00:19:22,960
+فيها قليل ليش؟ لإن هنا ماعنديش iron كفايةفال .. ال
+
+241
+00:19:22,960 --> 00:19:26,400
+homoglobin اللي بيتصنع ما بيكونش ما .. إله iron
+
+242
+00:19:26,400 --> 00:19:32,140
+فبالتالي بيكون فيه نقص في ال .. ال iron فبت .. في
+
+243
+00:19:32,140 --> 00:19:36,760
+ال homoglobin كمان و نقص في ال oxygen delivery
+
+244
+00:19:36,760 --> 00:19:42,060
+الثلاثيمية هي طفرة في جينات ال .. ال homoglobin
+
+245
+00:19:42,060 --> 00:19:46,880
+فبيتم تصنيع .. بيصير فيه decrease production من ال
+
+246
+00:19:46,880 --> 00:19:52,610
+homoglobinأو ممكن يكون الطفرة أعطتني هموجلوبين
+
+247
+00:19:52,610 --> 00:19:55,890
+الشكل تبعه و التركيب تبعه abnormal فبالتالي
+
+248
+00:19:55,890 --> 00:20:00,570
+الوظيفة تبعته هتكون abnormal ما بتحمل يعني أكسجين
+
+249
+00:20:00,570 --> 00:20:04,930
+طيب ال macrocytic anemia أو ال macrocytic cells
+
+250
+00:20:04,930 --> 00:20:09,570
+طيب أنيميا نتجت عن خلايا كبيرة في الحجم هذه
+
+251
+00:20:09,570 --> 00:20:14,030
+بنشوفها في liver disease وفي الhypothyroidism تمام
+
+252
+00:20:14,030 --> 00:20:19,650
+يعنيخلايا الثيرويد خلايا الغداددرقية هرموناتها
+
+253
+00:20:19,650 --> 00:20:23,390
+قليلة بتنتج هرمونات قليلة هي معناه hypo hypo قليل
+
+254
+00:20:23,390 --> 00:20:28,870
+thyroidism يعني هرمونات الغداددرقية تمام ال liver
+
+255
+00:20:28,870 --> 00:20:33,590
+disease مشكلة في الكبد ممكن ينتج عنها ان الخلايا
+
+256
+00:20:33,590 --> 00:20:39,410
+بتكون macrocytic كبيرة طيب ممكن لما يكون في عندي
+
+257
+00:20:39,410 --> 00:20:43,890
+asynchronous in RBCs maturationيعني مافيش تناسق في
+
+258
+00:20:43,890 --> 00:20:48,470
+نضوج ال RBCs تمام؟ و هذا بنسميه megaloplastic
+
+259
+00:20:48,470 --> 00:20:52,230
+anemia و هذه ال megaloplastic anemia يعني خلايا
+
+260
+00:20:52,230 --> 00:20:56,230
+كبيرة تمام؟ و لكن في عندي anemia يعني مافيش
+
+261
+00:20:56,230 --> 00:21:01,330
+همجلبين او يعني انه مافيش RBCs كافية تمام؟
+
+262
+00:21:01,330 --> 00:21:05,190
+فبالتالي هذه بتنتج عن ال folate عن ال folic acid و
+
+263
+00:21:05,190 --> 00:21:08,930
+ال vitamin B12 deficienciesطبعا خلينا بس نحكي
+
+264
+00:21:08,930 --> 00:21:13,370
+حدوطة صغيرة عشان نعرف إيش يعنيه إنه مافيش تناسق
+
+265
+00:21:13,370 --> 00:21:19,730
+هلأ ال RBCs أثناء نضوجها في ال bone marrow بتمر
+
+266
+00:21:19,730 --> 00:21:23,450
+بعدة مراحل بتكون عندي خلية أم إحنا متفقين إنه في
+
+267
+00:21:23,450 --> 00:21:27,890
+عندي خلية أم لكل نوع من أنواع ال blood cells فعيني
+
+268
+00:21:27,890 --> 00:21:33,260
+في عندي خلية أم ل ال RBCsهذه الخلية الأم مجرد ما
+
+269
+00:21:33,260 --> 00:21:39,760
+الجسم يحتاج RBCs بيرسللها رسالة فبتتنشط و بتبدأ
+
+270
+00:21:39,760 --> 00:21:44,100
+تنقسم و تندوج لحتى تعطيني RBCs و ال RBCs هادي
+
+271
+00:21:44,100 --> 00:21:47,120
+بتتخرج و بتطلع من ال .. من ال boomer و بتطلع ع ال
+
+272
+00:21:47,120 --> 00:21:53,060
+circulation تمام؟ طيب .. هلأ أثناء ندوج الخلايا
+
+273
+00:21:53,060 --> 00:21:58,630
+هاي ال ..اللي خلّيني نحكي عنها ال progenitors اللي
+
+274
+00:21:58,630 --> 00:22:04,370
+هي يعني الخلايا السلالة الخلايا الأصل لل RBCs
+
+275
+00:22:04,370 --> 00:22:11,490
+تمام؟ أثناء نضوجها بتكون إلها نواة يعني لما بتصل
+
+276
+00:22:11,490 --> 00:22:15,150
+لآخر مراحل في النضوج بتفقد النواة هذه عشان هيك ال
+
+277
+00:22:15,150 --> 00:22:21,310
+RBCs بتطلع على ال .. ال circulation تمام؟ بتطلع
+
+278
+00:22:21,310 --> 00:22:30,420
+فاقدة نواتها مافيش فيها نواة تمام؟و ال .. أثناء
+
+279
+00:22:30,420 --> 00:22:36,420
+أيضا ندوجها الخلية بيصغر حجمها، تمام؟ بتكون كبيرة،
+
+280
+00:22:36,420 --> 00:22:39,560
+الخلايا ال mother، الأم في المراحل الأولى من
+
+281
+00:22:39,560 --> 00:22:43,540
+الندوج بتكون كبيرة، بتبدأ تصغر، تصغر، تصغر، بعدين
+
+282
+00:22:43,540 --> 00:22:48,740
+بتفقد النواة، بعدين بتطلع على ال circulationإذن في
+
+283
+00:22:48,740 --> 00:22:53,060
+عندي أحداث بت .. بتصير أثناء ال maturation اللي هي
+
+284
+00:22:53,060 --> 00:22:56,820
+أول حاجة صغر في الحجم تدريجي لحد ما تصل لعندي ال
+
+285
+00:22:56,820 --> 00:23:00,860
+MCV ال normal اللي هي من 80 ل 100 و بعدين بتطلع ال
+
+286
+00:23:00,860 --> 00:23:09,270
+circulation و أيضا ..بيكون في فقدان للنواة و أيضا
+
+287
+00:23:09,270 --> 00:23:15,470
+بيكون في عندى ال homoglobin .. تصنيع لل
+
+288
+00:23:15,470 --> 00:23:19,170
+homoglobin، تمام؟ homoglobin synthesis فال
+
+289
+00:23:19,170 --> 00:23:23,210
+homoglobin synthesis هذا ما بيضل شغال على طول، لأ
+
+290
+00:23:23,980 --> 00:23:27,480
+الخلية لما بتطلع من ال .. ال .. ال bone marrow
+
+291
+00:23:27,480 --> 00:23:32,120
+لازم تكون وصلة لعين تركيز معين من ال homoglobin E
+
+292
+00:23:32,120 --> 00:23:35,760
+كف .. كفيل انه يخرجها من ال bone marrow و تروح ع
+
+293
+00:23:35,760 --> 00:23:42,560
+ال circulation، طبعا، احنا هناكطلاب مثلا بتدرسوا،
+
+294
+00:23:42,560 --> 00:23:47,560
+بتنضجوا، بتتعلموا، بتكتسبوا خبرات، تمام؟ بتجتازوا
+
+295
+00:23:47,560 --> 00:23:52,480
+امتحانات و لما نصل لعند مرحلة معينة من العلم عندكم
+
+296
+00:23:52,480 --> 00:23:56,660
+كافية أننا نخرجكم، تتخرجوا، تمام؟ نفس الاشي ال
+
+297
+00:23:56,660 --> 00:24:05,460
+RBCsبتنضج، بتنقسم و بتفقد النواء تبعتها، بتصنع
+
+298
+00:24:05,460 --> 00:24:09,220
+هموجلوبين، الهموجلوبين هذا بيصل لمستوى كافي إنها
+
+299
+00:24:09,220 --> 00:24:14,040
+تطلع من ال bone marrow فتطلع، تمام؟ طيبلو كان في
+
+300
+00:24:14,040 --> 00:24:18,560
+عندي نقص في ال .. ال iron طبعا، لو كان في عندي نقص
+
+301
+00:24:18,560 --> 00:24:21,580
+في ال iron أو حتى نقص في تصنيع ال homoglobin ذات
+
+302
+00:24:21,580 --> 00:24:26,880
+نفسه، الخلية بتضلها تنقسم، تنقسم، تنقسم ل .. لحد
+
+303
+00:24:26,880 --> 00:24:30,240
+.. يعني بدها تصل لمرحلة إنه يكون ال homoglobin
+
+304
+00:24:30,240 --> 00:24:35,020
+اللي موجود فيها كافي إنها تتخرج، طبعا؟ كافي إنها
+
+305
+00:24:35,020 --> 00:24:39,500
+تطلع من ال bone marrowطبعا فبتضطر انها تبضل تنقسم
+
+306
+00:24:39,500 --> 00:24:45,220
+تنقسم لحد ما تصل لحجم كتير صغير عليها انه يكون ال
+
+307
+00:24:45,220 --> 00:24:49,220
+homoglobin اللي فيها كافي انها تطلع عشان يطلعها ال
+
+308
+00:24:49,220 --> 00:24:55,610
+bone marrow طبعا فعشان هيك في حالة نقصان ال ironو
+
+309
+00:24:55,610 --> 00:24:59,150
+في حال اتخلل في تصنيع ال homoglobin او نقصان في
+
+310
+00:24:59,150 --> 00:25:02,630
+تصنيع ال homoglobin تمام مافيش عندي homoglobin
+
+311
+00:25:02,630 --> 00:25:05,470
+كافي في ال bone marrow .. في ال .. ال RBCs في ال
+
+312
+00:25:05,470 --> 00:25:10,090
+bone marrow تمام فبالتالي ال RBCs اش اللي عمل
+
+313
+00:25:10,090 --> 00:25:15,330
+امامها ان هتظلها تنقسمتمام؟ انقسامات وانقسامات
+
+314
+00:25:15,330 --> 00:25:19,670
+وانقسامات لحد ما تصل لحجم كتير صغير ما هي كل ما
+
+315
+00:25:19,670 --> 00:25:24,250
+بتنقسم كل ما بصغر حجمها تمام؟ لحد ما تصل لحجم كتير
+
+316
+00:25:24,250 --> 00:25:28,350
+صغير و يكون شوية ال homoglobin اللي فيها كافية
+
+317
+00:25:28,350 --> 00:25:32,170
+عليها بالنسبة لحجمها طبعا كافية انها تتخرج و تطلع
+
+318
+00:25:32,170 --> 00:25:36,530
+من ال bone marrow تمام؟ فعشان هيك ال iron
+
+319
+00:25:36,530 --> 00:25:41,880
+deficiency و الthalassemiaبينتج عنها microcytic
+
+320
+00:25:41,880 --> 00:25:47,320
+cell خلية ال MCV تبعها قليل عرفنا ليش بسبب
+
+321
+00:25:47,320 --> 00:25:52,680
+الانقسامات الكثيرة لحتى تصل لل target تبعها اللي
+
+322
+00:25:52,680 --> 00:25:55,880
+هو ال homoglobin content اللي لازم تكون على الأقل
+
+323
+00:25:55,880 --> 00:26:02,400
+محتوية بالنسبة لحجمها طيبفي حالة الفولك أسد
+
+324
+00:26:02,400 --> 00:26:07,480
+ديفشانسي و ال vitamin B12 ديفشانسي إيش بيصير؟ طبعا
+
+325
+00:26:07,480 --> 00:26:13,600
+هدول يا بنات بيلزموا لانقسام النواء تمام؟ بيلزموا
+
+326
+00:26:13,600 --> 00:26:18,360
+الخلية أثناء انقسام النواء طبعتها طيب هدول تم
+
+327
+00:26:18,360 --> 00:26:23,380
+نقسانهم إذا النواء مش راح تنقسم إذا الخلية راح
+
+328
+00:26:23,380 --> 00:26:29,250
+تظلها كبيرةو احنا حاكينا كل ما تنقسم كل ما زغرت في
+
+329
+00:26:29,250 --> 00:26:36,130
+الحجم طيب في عندي عوامل أصلا يعني متطلبات و مهمة
+
+330
+00:26:36,130 --> 00:26:39,690
+جدا لانقسام النواة و في عندي deficiencies فيهم، في
+
+331
+00:26:39,690 --> 00:26:44,810
+نقصان فيهم، تمام؟طيب فبالتالي النواء مش راح تنقسم
+
+332
+00:26:44,810 --> 00:26:49,870
+فالخلية ممكن تفقد .. يعني راح تبقى تصنع homoglobin
+
+333
+00:26:49,870 --> 00:26:54,250
+و تصل ال target بس لسه حجمها كبير فبتفقد النواء
+
+334
+00:26:54,250 --> 00:27:00,450
+early تمام؟ بتم التخلص من نواتها و هي لسه حجمها
+
+335
+00:27:00,450 --> 00:27:06,600
+كبير تمام؟فهذا بنسميه asynchronous عدم تناسق ما
+
+336
+00:27:06,600 --> 00:27:10,420
+بين انقسام النواء ونضوج ال .. او ال .. ال
+
+337
+00:27:10,420 --> 00:27:13,180
+cytoplasm يعني انه هو يعني انه يكون فيه محتوى
+
+338
+00:27:13,180 --> 00:27:18,780
+هموجلوبين كافي تمام؟ فبينتج عنه اما microcytic او
+
+339
+00:27:18,780 --> 00:27:23,000
+macrocytic cells تمام؟ هذا ال asynchronous بينتج
+
+340
+00:27:23,000 --> 00:27:29,800
+عنه microcytic او macrocytic اذا تأخرتصنيع ال
+
+341
+00:27:29,800 --> 00:27:35,380
+homoglobin الكافي لتخريج ال RBCs من ال bone marrow
+
+342
+00:27:35,380 --> 00:27:41,140
+تستمر الخلية في الانقسام لحد ما تصل ل microcytic
+
+343
+00:27:41,140 --> 00:27:48,380
+تمام؟ وإذا تأخر نضوج النواء وانقسام النواءتستمر
+
+344
+00:27:48,380 --> 00:27:54,280
+الخلية بتصنيع ال homoglobin ولكن بعد هي بتضطر ..
+
+345
+00:27:54,280 --> 00:27:57,280
+بتصلح .. لا خلاص بتصلح ال target اللي كافيل بإنه
+
+346
+00:27:57,280 --> 00:28:00,920
+يخرجها من ال bone marrow فبتضطر إنها تتخلص من
+
+347
+00:28:00,920 --> 00:28:04,480
+النواء و هي لساتها حجمها كبير لإنها ما انقسمت
+
+348
+00:28:04,480 --> 00:28:09,020
+كفاية لإن العوامل اللي بتلزمها لانقسم نواتها أصلا
+
+349
+00:28:09,020 --> 00:28:13,620
+فيهم deficiency فالخلية بتطلع ليه macrocytic تمام؟
+
+350
+00:28:13,620 --> 00:28:19,980
+بتطلع macrocytic كبيرةطيب ال mean corpuscular
+
+351
+00:28:19,980 --> 00:28:25,880
+homoglobin متوسط ال homoglobin في الكورية طبعا؟ طب
+
+352
+00:28:25,880 --> 00:28:29,940
+متوسط ال homoglobin ايش؟ وزن ال homoglobin طبعا؟
+
+353
+00:28:29,940 --> 00:28:34,480
+the average weight of homoglobin in an RBCs طيب
+
+354
+00:28:34,480 --> 00:28:39,320
+وحدة القياس ال picogram طبعا؟ ال picogram و ال
+
+355
+00:28:39,320 --> 00:28:43,620
+picogram هو عبارة عن عشرة أسالب اتناش ملي جرام طيب
+
+356
+00:28:44,640 --> 00:28:50,320
+خلّينا نشوف طيب كتع .. يعني كتحليل منطقي لقى
+
+357
+00:28:50,320 --> 00:28:58,700
+القانون نشوف إيش يعني MCH ال MCH إحنا حكينا متوسط
+
+358
+00:28:58,700 --> 00:29:05,200
+وزن ال homoglobin في ال .. ال RBCs الواحدة تمام؟
+
+359
+00:29:05,200 --> 00:29:11,280
+طيب إحنا لما كنا نقيس ال homoglobin .. sorryلما
+
+360
+00:29:11,280 --> 00:29:14,720
+كنا نقيس ال homoglobin ايش كنا نحكي؟ كنا نحكي ال
+
+361
+00:29:14,720 --> 00:29:19,540
+total homoglobin ايش يعني؟ يعني ال homoglobin اللي
+
+362
+00:29:19,540 --> 00:29:24,620
+في كل خلايا الدم per microliter صح؟ تمام يعني انه
+
+363
+00:29:24,620 --> 00:29:29,060
+انا بنهاية كنت لما احصل على ال homoglobin كتركيز
+
+364
+00:29:29,060 --> 00:29:33,920
+كنت احصل عليه بوحدة gram per deciliter تمام؟ عدد
+
+365
+00:29:33,920 --> 00:29:39,380
+الخلايا بال deciliter تمام؟ كلها هذه انا أخدت ال
+
+366
+00:29:39,380 --> 00:29:46,700
+homoglobin اللي فيهاو طلعت وزنه طب أنا لو عارفة
+
+367
+00:29:46,700 --> 00:29:53,560
+الخلايا هذه عددها ككل مش ممكن يعني أقسم وزن ال ..
+
+368
+00:29:53,560 --> 00:29:57,960
+ال .. ال homoglobin ككل على عدد الخلايا ككل
+
+369
+00:29:57,960 --> 00:30:03,770
+بيطلعلي وزن ال homoglobin في الخلية الواحدةفإذا
+
+370
+00:30:03,770 --> 00:30:07,690
+هذا هو تفسير القانون اللي هو مجلبين ككل بال gram
+
+371
+00:30:07,690 --> 00:30:13,630
+per deciliter على ال RBCs count ككل و درب عشرة
+
+372
+00:30:13,630 --> 00:30:19,610
+طبعا فال normal range بيطلع من 28 ل 32 picogram
+
+373
+00:30:20,640 --> 00:30:26,180
+طبعا ال MCH نادرا أو يعني مش عادة بيستخدم لتصنيف
+
+374
+00:30:26,180 --> 00:30:30,540
+ال anemia ولكنه أحيانا بيكون مؤشر جيد عن محتوى ال
+
+375
+00:30:30,540 --> 00:30:36,080
+homoglobin في داخل ال RBCs كوزن تمام؟ هاي هنا مثال
+
+376
+00:30:36,080 --> 00:30:41,840
+كتطبيق للقانون ال homoglobin 16 ال RBCs 5 مليون
+
+377
+00:30:41,840 --> 00:30:47,270
+طبعا أنا باخد كام مليون؟ 5 خلاص بعود في الخمسةطيب
+
+378
+00:30:47,270 --> 00:30:51,330
+16 على 5 ده 14 بيطلع عندى طبعا 16 على 5 ثلاثة
+
+379
+00:30:51,330 --> 00:30:55,870
+point اتنين ال .. في عشرة اتنين و تلاتين picogram
+
+380
+00:30:55,870 --> 00:31:01,070
+تمام؟ و نفس ال issue إذا كان ال .. ال .. ال MCH
+
+381
+00:31:01,070 --> 00:31:08,510
+أقل من 28 it is low و إذا كان أكثر من 32 it's high
+
+382
+00:31:08,510 --> 00:31:14,150
+طيب؟ولكن احنا حكينا مش دايما انا بستخدموا فيه
+
+383
+00:31:14,150 --> 00:31:17,090
+classification للانيميا في تصنيف الانيميا او في
+
+384
+00:31:17,090 --> 00:31:21,750
+وصف الانيميا طيب ال mean carbocular homoglobin
+
+385
+00:31:21,750 --> 00:31:27,630
+concentration اللي هو ال MCHC هو عبارة عن متوسط
+
+386
+00:31:27,630 --> 00:31:32,030
+تمام ال homoglobin concentration متوسط تركيز ال
+
+387
+00:31:32,030 --> 00:31:35,150
+homoglobin في الكورية الواحدة او في الخلية الواحدة
+
+388
+00:31:35,460 --> 00:31:38,860
+فإذن هو عبارة عن the average concentration متوسط
+
+389
+00:31:38,860 --> 00:31:43,420
+التركيز لل homoglobin في كل خلية من خلايا ال .. ال
+
+390
+00:31:43,420 --> 00:31:48,500
+.. ال RBCs تمام؟ في الخلية الواحدة طيب فبن .. ال
+
+391
+00:31:48,500 --> 00:31:53,480
+.. وحدة القياس طبعا بتكون as a percentage أو ممكن
+
+392
+00:31:53,480 --> 00:31:57,840
+أن أنا أحكي عنها gram per ال deciliter تمام؟ طيب
+
+393
+00:31:59,450 --> 00:32:03,910
+طبعا ال .. ال MCHC قانونه ال homoglobin على ال
+
+394
+00:32:03,910 --> 00:32:08,810
+hematocrit ضارب 100% تمام؟ تركيز ال homoglobin ككل
+
+395
+00:32:08,810 --> 00:32:13,150
+على حجم اللي بتحتله ال RBCs فهنا بحصل على كام ال
+
+396
+00:32:13,150 --> 00:32:16,110
+.. النسبة ال .. ال homoglobin في الخلية الواحدة
+
+397
+00:32:16,110 --> 00:32:22,230
+تمام؟ فهي هنا مثال 16 ال homoglobin على 48 ال
+
+398
+00:32:22,230 --> 00:32:26,570
+hematocrit ضارب 100 فبيطلعلي 33.3 gram per
+
+399
+00:32:26,570 --> 00:32:35,210
+deciliter أو as a percentage حكي هناطيب ال ..
+
+400
+00:32:35,210 --> 00:32:38,430
+الخلية
+
+401
+00:32:38,430 --> 00:32:43,390
+الطبيعية بتحتوي على تركيز من الهموجلوبين ما بين 32
+
+402
+00:32:43,390 --> 00:32:48,870
+ل 37 gram per deciliter طبعا؟ طيب و هذه الخلية بما
+
+403
+00:32:48,870 --> 00:32:55,270
+أنها طبيعية بنصفها كأنها normal .. chromic طبعا؟
+
+404
+00:32:55,270 --> 00:33:00,760
+normal يعني normalو chromic لون، إذا لونها طبيعي،
+
+405
+00:33:00,760 --> 00:33:04,180
+يعني ال homoglobin اللي أصلا بيعطي ال RBC's اللون
+
+406
+00:33:04,180 --> 00:33:08,320
+طبيعي، تمام؟ تركيزوا طبيعي في الخلية طيب، بس هنا
+
+407
+00:33:08,320 --> 00:33:12,400
+جادير بتذكر إنه هنا بنوّهلي إنه زمان كانوا يعبروا
+
+408
+00:33:12,400 --> 00:33:18,020
+عن ال MCHC as a percentage و حاليا الوحدة الدرجة
+
+409
+00:33:18,020 --> 00:33:23,510
+هي gram per deciliter، تمام؟طيب hypochromic إيش
+
+410
+00:33:23,510 --> 00:33:26,950
+يعني hypochromic؟ hypo قليل، chromic لون، إذا
+
+411
+00:33:26,950 --> 00:33:30,670
+التركيز الهوموجلوبين هيكون أقل من 32 gram per
+
+412
+00:33:30,670 --> 00:33:35,710
+deciliter طيب hyper، hyper عالي، chromic لون،
+
+413
+00:33:35,710 --> 00:33:39,390
+hypochromic معناها تركيز الهوموجلوبين أعلى من 37
+
+414
+00:33:39,390 --> 00:33:44,220
+gram per deciliter، تمام؟ طيبالـ hypochromic
+
+415
+00:33:44,220 --> 00:33:47,720
+erythrocyte occur in thalassemia and iron
+
+416
+00:33:47,720 --> 00:33:51,200
+deficiency anemia و هذا الاشي صار بديهي بالنسبة
+
+417
+00:33:51,200 --> 00:33:53,660
+لإن حكينا إن ال thalassemia و ال iron deficiency
+
+418
+00:33:53,660 --> 00:33:58,520
+anemia بيكون فيهم الخلايا microcytic صغيرة و عرفنا
+
+419
+00:33:58,520 --> 00:34:02,640
+ليش صغيرة تمام؟ و بتكون أيضا hypochromicويعرفنا
+
+420
+00:34:02,640 --> 00:34:05,520
+ليش؟ لإن العلة أصلا في تصنيع ال homoglobin إما
+
+421
+00:34:05,520 --> 00:34:08,800
+مافيش ironic فاية أو في خلل أو قلة في تصنيع ال
+
+422
+00:34:08,800 --> 00:34:12,700
+homoglobin تمام؟ فإذا هتكون بالإضافة لإن هتكون
+
+423
+00:34:12,700 --> 00:34:16,360
+microcytic بسبب ال asynchronous في انقسام الخلية
+
+424
+00:34:16,360 --> 00:34:22,040
+هتكون أيضا hypoachromicطيب شوفوا معايا، هلأ إحنا
+
+425
+00:34:22,040 --> 00:34:26,960
+حكينا إنه في عندي level معين لل homoglobin إنه
+
+426
+00:34:26,960 --> 00:34:31,260
+الخلية لما بتصل لعنده بتتخرج من ال bone marrow و
+
+427
+00:34:31,260 --> 00:34:33,580
+بتطلع على ال circulation هذا ال level اللي هو ما
+
+428
+00:34:33,580 --> 00:34:40,520
+بين 32 ل 37 gram per deciliter، تمام؟ يعني مستحيل
+
+429
+00:34:40,520 --> 00:34:44,460
+الخلية تصل أعلى من هيكإذا مافيش عندي حاجة اسمها
+
+430
+00:34:44,460 --> 00:34:48,060
+hyperchromic طبعا مافيش خلية بيتم إنتاجها ك
+
+431
+00:34:48,060 --> 00:34:51,460
+hyperchromic يعني فيها يعني بتحتوي على homoglobin
+
+432
+00:34:51,460 --> 00:34:56,000
+concentration أعلى من 37 gram per deciliter طيب
+
+433
+00:34:56,000 --> 00:35:00,080
+إيش يعني؟ طب ليش أمتى بنستخدم hyperchromic؟
+
+434
+00:35:00,080 --> 00:35:05,660
+hyperchromic لما بيتغير شكل الخلية وبيتغير حجمها
+
+435
+00:35:05,850 --> 00:35:10,670
+فأيضا بيتغير توزيع ال homoglobin فيها فبنسميها ..
+
+436
+00:35:10,670 --> 00:35:13,390
+بتبطل by concave و discotheque بتصير spherical
+
+437
+00:35:13,390 --> 00:35:18,610
+بتصير زي الكرة تمام مدورة من كل الجهات فعايش ال ..
+
+438
+00:35:18,610 --> 00:35:23,740
+ال .. في هاي الحالة بتبين كأنها fullيعني حتى كمان
+
+439
+00:35:23,740 --> 00:35:27,220
+لما بشوفها على ال blood film مابشوفش منطقة ال
+
+440
+00:35:27,220 --> 00:35:30,100
+central baller بشوف ان ال homoglobin مغطيها كلها
+
+441
+00:35:30,100 --> 00:35:34,700
+تمام فهذه الحالة باصفها كوصف انها hyperchromic
+
+442
+00:35:34,700 --> 00:35:38,360
+ولكن لازم اعرف انه هي يعني ال homoglobin
+
+443
+00:35:38,360 --> 00:35:42,980
+concentration فيها لا يتجاوز ال 37 gram per
+
+444
+00:35:42,980 --> 00:35:49,280
+deciliter وهذه المعلومة احنا ذكرناهافي محاضرة ل
+
+445
+00:35:49,280 --> 00:35:55,200
+blood film حكينا إن ال RBCs بتكون طبعا فيها منطقة
+
+446
+00:35:55,200 --> 00:35:59,400
+تلتها في المركز بيكون في central pallor شحوب مركزي
+
+447
+00:35:59,400 --> 00:36:04,580
+منطقة خالية من ال homoglobin لو كانت الخلية طبعا
+
+448
+00:36:04,580 --> 00:36:07,620
+ما .. لا تحتوي على central pallor إذا هي
+
+449
+00:36:07,620 --> 00:36:13,120
+spherocytic تمام؟وإذا هي hyperchromic تجاوزا بصفها
+
+450
+00:36:13,120 --> 00:36:17,220
+ك hyperchromic لإنها it's as big as full تظهر
+
+451
+00:36:17,220 --> 00:36:21,020
+كإنها مليانة هموجلبين ولكن فعليا لا يتجاوز ل 37
+
+452
+00:36:21,020 --> 00:36:27,560
+gram per deciliter طبعا فإذا حسبنا MCHC أعلى من 37
+
+453
+00:36:27,560 --> 00:36:31,700
+gram per deciliter فهذا معناها إنه الهموجلبين
+
+454
+00:36:31,700 --> 00:36:37,020
+value تركيز الهموجلبين هناالهموجلبين قلي .. عالي
+
+455
+00:36:37,020 --> 00:36:40,940
+طبعا في error عالي البسط عالي يعني ال mchc هيطلع
+
+456
+00:36:40,940 --> 00:36:47,100
+عالي طبعا فمعناها في عندى error في الهموجلبين
+
+457
+00:36:47,100 --> 00:36:55,480
+value طيب اذا mcv normal mchc normal طبعا un يعني
+
+458
+00:36:55,480 --> 00:37:07,190
+normal اذا هي normocyticnormal normal normal
+
+459
+00:37:07,190 --> 00:37:07,250
+normal normal normal normal normal normal normal
+
+460
+00:37:07,250 --> 00:37:07,830
+normal normal normal normal normal normal normal
+
+461
+00:37:07,830 --> 00:37:08,110
+normal normal normal normal normal normal normal
+
+462
+00:37:08,110 --> 00:37:08,430
+normal normal normal normal normal normal normal
+
+463
+00:37:08,430 --> 00:37:10,370
+normal normal normal normal normal normal normal
+
+464
+00:37:10,370 --> 00:37:18,630
+normal normal normal normal normal
+
+465
+00:37:18,630 --> 00:37:18,670
+normal normal normal normal normal normal normal
+
+466
+00:37:18,670 --> 00:37:18,750
+normal normal normal normal normal normal normal
+
+467
+00:37:18,750 --> 00:37:18,790
+normal normal normal normal normal normal normal
+
+468
+00:37:18,790 --> 00:37:21,710
+normal normal normal normal normalطيب لو كان في
+
+469
+00:37:21,710 --> 00:37:24,930
+عندي decrease في التنتين إيش بدي أوصف ال anemia؟
+
+470
+00:37:24,930 --> 00:37:28,390
+بدي أحكي عن إنه قلة في ال MCV أو قلة في الحجم أو
+
+471
+00:37:28,390 --> 00:37:35,310
+صغر في الحجم يعني decrease MCV microcytic حكينا ب
+
+472
+00:37:35,310 --> 00:37:41,000
+.. بعبّر عنها كإنها microcytic بستخدم هذا اللفظطيب
+
+473
+00:37:41,000 --> 00:37:45,220
+قلة في اللون او يعني hemoglobin concentration قليل
+
+474
+00:37:45,220 --> 00:37:48,740
+ايضا decrease طبعا دي يعني decrease اذا
+
+475
+00:37:48,740 --> 00:37:52,900
+hypochromic حكينا اذا بسميها microcytic
+
+476
+00:37:52,900 --> 00:37:56,460
+hypochromic anemia ومثال على ال microcytic
+
+477
+00:37:56,460 --> 00:37:59,820
+hypochromic anemia اللي هي اما iron deficiency
+
+478
+00:37:59,820 --> 00:38:05,420
+anemia او ثلاثيميا طبعا طيب macrocytic
+
+479
+00:38:06,390 --> 00:38:12,250
+يعني إيش؟ ال MCV عالي increase تمام؟ و
+
+480
+00:38:12,250 --> 00:38:18,470
+normochromic يعني ال MCHC normal تمام؟ فهذا معنى
+
+481
+00:38:18,470 --> 00:38:21,470
+إنه الخلية أو الأنينيا عندي macrocytic
+
+482
+00:38:21,470 --> 00:38:26,670
+normochromic طبعا في عندي أسماء من ال .. ال .. أو
+
+483
+00:38:26,670 --> 00:38:32,110
+أنواع من الأنينيات اللي بيندرجوا تحت هاي ال ..
+
+484
+00:38:32,110 --> 00:38:39,140
+الوصف تحت هدول الأوصافتمام؟ فبالتالي الخطوة الأولى
+
+485
+00:38:39,140 --> 00:38:49,140
+إن أنا أقيم شكل ال .. ال .. ال RBCs و أشوف مؤشرات
+
+486
+00:38:49,140 --> 00:38:55,940
+ال RBCs هذه تمام؟أوصف نوع الأنيميا فبالتالي
+
+487
+00:38:55,940 --> 00:38:59,660
+بتحصرلي أنواع معينة مثلا normocytic-normochromic
+
+488
+00:38:59,660 --> 00:39:03,520
+في عندي أنواع معينة واحد اتنين تلاتة فبضل .. خلاص
+
+489
+00:39:03,520 --> 00:39:07,800
+بنحصر بي هدول الأنواع ببدأ أعمل احوصات تانية تفرق
+
+490
+00:39:07,800 --> 00:39:10,860
+ما بين الأنواع تبعوني normocytic-normochromic
+
+491
+00:39:10,860 --> 00:39:18,640
+تمام؟ كانت مؤشرات وشكل ال .. ال RBCsو ال indices
+
+492
+00:39:18,640 --> 00:39:22,640
+اللي انا حسبتهم بيحكولي انها microcytic
+
+493
+00:39:22,640 --> 00:39:25,860
+hypochromic اذا انا هتوجه اما هتكون ثلاثيميا او
+
+494
+00:39:25,860 --> 00:39:30,200
+iron deficiency anemia ممكن اعمل فحوصات لمستوى ال
+
+495
+00:39:30,200 --> 00:39:34,160
+iron ممكن يعني اعمل فحوصات لحتى اكشف عن جيناتي ال
+
+496
+00:39:34,160 --> 00:39:37,400
+homoglobin و اشوف اذا فيها خلل عشان اشخص ثلاثيميا
+
+497
+00:39:37,400 --> 00:39:43,320
+يعني فبالتالي اكون محصورة بالانواع اللي هي
+
+498
+00:39:43,320 --> 00:39:46,820
+microcytic hypochromicطمام؟ macrocyclic
+
+499
+00:39:46,820 --> 00:39:50,480
+-normochromic برضه كمان نفس الأشياء فبالتالي إذا
+
+500
+00:39:50,480 --> 00:39:54,720
+حكينا إنها بتساعدني في وصف نوع الأنيميا و ال
+
+501
+00:39:54,720 --> 00:39:57,840
+classification و ال differential diagnosis التفريق
+
+502
+00:39:57,840 --> 00:40:06,790
+ما بين هاي الأنيمياتتمام؟ طيب هاي مصطلحات راح ان
+
+503
+00:40:06,790 --> 00:40:12,590
+نذكرها already صيرنا بنعرفها بس خلوني بس أحكي
+
+504
+00:40:12,590 --> 00:40:20,880
+نقطتين أول إشي ال ..بنهاية المحاضرة هاي احنا
+
+505
+00:40:20,880 --> 00:40:27,400
+تقريبا صرنا بنعرف نشتغل و بنعرف نحسب كل بنود ال
+
+506
+00:40:27,400 --> 00:40:31,160
+CBC اللي هو ال complete blood count تمام؟ ال
+
+507
+00:40:31,160 --> 00:40:36,680
+complete blood count بيكون فيه عد ل ال YPCs و
+
+508
+00:40:36,680 --> 00:40:41,060
+بيكون فيه عد ل ال RPCs و احنا اتعرفناهم و يعرفنا
+
+509
+00:40:41,060 --> 00:40:48,500
+كيف بنشتغلهم و كيف بنحسبهم manual تمام؟و أيضًا
+
+510
+00:40:48,500 --> 00:40:53,200
+بيكون فيه differential ل ال white pieces يعني كام
+
+511
+00:40:53,200 --> 00:40:55,960
+نسبة ال monocyte، كام نسبة ال lymphocyte، ال
+
+512
+00:40:55,960 --> 00:40:59,420
+neutrophil، ال eosinophil، ال basophil، تمام،
+
+513
+00:40:59,420 --> 00:41:04,280
+فبرضه هذه أنا أعطيتكم فكرة عنها إنه إحنا من خلال
+
+514
+00:41:04,280 --> 00:41:09,280
+blood film نحسب، يعني بنعيد 100 خلية و بنشوف كام
+
+515
+00:41:09,280 --> 00:41:11,680
+طلع معنا منهم ال neutrophil، كام طلع معنا منهم
+
+516
+00:41:11,680 --> 00:41:16,670
+monocyte، و كام lymphocyteلأخرين و بنحسب نسبة
+
+517
+00:41:16,670 --> 00:41:21,970
+المئوية لإنهم تمام طيب و أيضا بيكون في حساب لل
+
+518
+00:41:21,970 --> 00:41:25,010
+homoglobin concentration و سرنا بنعرف بطريقة
+
+519
+00:41:25,010 --> 00:41:30,790
+draken method بنعرف نحسب تركيز ال homoglobin برضه
+
+520
+00:41:30,790 --> 00:41:36,830
+manual يدويوبيكون في حساب لتركيز او عدد ال
+
+521
+00:41:36,830 --> 00:41:40,390
+bloodlet الصفائح الدموية من خلال blood film يمكن
+
+522
+00:41:40,390 --> 00:41:46,390
+عدها ايضا فإذا احنا صيرنا بنعرف تقريبا نعمل CBC
+
+523
+00:41:46,390 --> 00:41:51,070
+يدوي صح؟ و بس مسألة ال difference يعني لو عدت ال
+
+524
+00:41:51,070 --> 00:41:54,850
+bloodlet هتاخدوها اكثر بشكل موسع في ال hematology
+
+525
+00:41:56,980 --> 00:42:02,440
+اللي بيفرق انه ال CPC بيكون automated جهاز بيشتغله
+
+526
+00:42:02,440 --> 00:42:05,820
+تمام؟ ولكن احنا بنفرق عن الجهاز يعني انا في
+
+527
+00:42:05,820 --> 00:42:09,340
+النهاية ممكن الجهاز يغلط لكن انا لازم اكون فاهمة و
+
+528
+00:42:09,340 --> 00:42:13,080
+عارفة المبدأ او من وين اجت الحسابات و كيف الجهاز
+
+529
+00:42:13,080 --> 00:42:16,380
+اصلا بيعد و كيف بيشتغل فدي كلها لازم نكون احنا
+
+530
+00:42:16,380 --> 00:42:19,940
+بنعرفها عشان هيك احنا اتعلمناالطريقة اليدوية
+
+531
+00:42:19,940 --> 00:42:24,520
+الجهاز بيستخدم المعادلات هاي لحتى يحسب ال indices
+
+532
+00:42:24,520 --> 00:42:29,060
+و بيطلعلي mcb و mch و mchc و هيكم انتوا اتعلمتوا
+
+533
+00:42:29,060 --> 00:42:34,820
+كمان اليوم ان كيف تحسبوهم فبالتالي اذا يعني صار في
+
+534
+00:42:34,820 --> 00:42:38,780
+عندنا رؤية واضحة انا كيف ممكن اعمل complete blood
+
+535
+00:42:38,780 --> 00:42:41,000
+count لشخص يدويا
+
+536
+00:42:43,450 --> 00:42:47,050
+طيب خلّينا نشوف إيش يعني anemia مرت معانا إنه هي
+
+537
+00:42:47,050 --> 00:42:50,490
+عبارة عن conditions، ظروف، variety of condition
+
+538
+00:42:50,490 --> 00:42:55,750
+طبعا، متنوعة هذه الظروف المحصلة إن ال person's
+
+539
+00:42:55,750 --> 00:42:59,350
+blood دم الشخص can't carry as much oxygen as it
+
+540
+00:42:59,350 --> 00:43:03,870
+should يعني مابيحملش oxygen كما ينبغي اللي إما
+
+541
+00:43:03,870 --> 00:43:08,330
+بيكون سبب إنه ال number أو ال size أو ال
+
+542
+00:43:08,330 --> 00:43:15,130
+homoglobin content لل RBCs قليل طبعاوالحالة يعني
+
+543
+00:43:15,130 --> 00:43:21,150
+بيكون المحصلة hypoxia تمام hypochromic حكينا hypo
+
+544
+00:43:21,150 --> 00:43:26,470
+نقصان أو قلة في ash اللون يعني قلة في تركيز ال
+
+545
+00:43:26,470 --> 00:43:31,490
+homoglobin إذا هو المصطلح اللي بيطبق على ال RBCs
+
+546
+00:43:31,490 --> 00:43:34,490
+لما بتكون decreased concentration of homoglobin
+
+547
+00:43:34,490 --> 00:43:39,090
+عندها نقص في تركيز ال homoglobinطبعا macrocytic
+
+548
+00:43:39,090 --> 00:43:43,330
+يعني خلية اش macro كبيرة, cytic خلية كبيرة, اذا
+
+549
+00:43:43,330 --> 00:43:47,370
+larger than normal red blood cell, main carboxylic
+
+550
+00:43:47,370 --> 00:43:51,590
+homoglobin هو ال average homoglobin طبعا weight,
+
+551
+00:43:51,910 --> 00:43:55,270
+وزن ال homoglobin في ال RBCs, ال main carboxylic
+
+552
+00:43:55,270 --> 00:43:57,150
+homoglobin concentration هو ال average
+
+553
+00:43:57,150 --> 00:44:01,110
+concentrationللهموجلبين في ال red blood cell ال
+
+554
+00:44:01,110 --> 00:44:03,990
+main corpuscular volume هو الأذرش volume لل red
+
+555
+00:44:03,990 --> 00:44:09,190
+blood cell طبعا micro حكيمه صغير و 6 خلية إذا خلية
+
+556
+00:44:09,190 --> 00:44:12,910
+smaller than normal ال MCV تبعها هيكون قليل
+
+557
+00:44:12,910 --> 00:44:16,970
+normochromic خلية اللون فيها طبيعي إذا ال red
+
+558
+00:44:16,970 --> 00:44:19,490
+blood cell عندها normal concentration of
+
+559
+00:44:19,490 --> 00:44:24,310
+homoglobinnormocytic يعني خلية حجمها طبيعي إذا ال
+
+560
+00:44:24,310 --> 00:44:28,390
+red blood cell normal size ال MCV normal و ال red
+
+561
+00:44:28,390 --> 00:44:32,930
+blood cell indices كشكل يعني كشكل عام إنها عبارة
+
+562
+00:44:32,930 --> 00:44:36,890
+عن المؤشرات والقياسات اللي بتفسر الحجم و ال
+
+563
+00:44:36,890 --> 00:44:40,030
+homoglobin content لل red blood cell تمام؟ و هذا
+
+564
+00:44:40,030 --> 00:44:44,010
+هو التعريف اللي إحنا بدأنا في محاضرتنا عم نختم فيه
+
+565
+00:44:44,010 --> 00:44:48,350
+هلأ و هيك بنكون وصلنا لنهاية المحاضرة يعطيكم ألف
+
+566
+00:44:48,350 --> 00:44:48,770
+عافية
+
diff --git a/PL9fwy3NUQKwY9jl8xw-bWkVZkWcGGyspd/Up-Q0W10hks_raw.json b/PL9fwy3NUQKwY9jl8xw-bWkVZkWcGGyspd/Up-Q0W10hks_raw.json
new file mode 100644
index 0000000000000000000000000000000000000000..54476d6d7b10668e4ea23359719e51bf8225682f
--- /dev/null
+++ b/PL9fwy3NUQKwY9jl8xw-bWkVZkWcGGyspd/Up-Q0W10hks_raw.json
@@ -0,0 +1 @@
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7, "seek": 17172, "start": 150.96, "end": 171.72, "text": "محتوها من الـ Homoglobin بالـ blood film عن طريق الـ morphology الشكلها طبعا أنا بحفظ شكلها خلية RBCs تلتينها Homoglobin تلتها Central Baller و بيصير حجمها مقلوف لإلي طبعا بالنسبة لل Red blood cell indices كيف أنا بقيم ال size و ال Homoglobin content من خلال", "tokens": [2304, 33753, 2407, 11296, 9154, 2423, 39184, 20903, 45841, 13496, 20666, 39184, 3390, 2007, 18871, 23032, 16572, 4587, 2423, 39184, 25778, 1793, 25124, 28820, 11296, 23032, 3555, 3615, 995, 41850, 4724, 5016, 5172, 19913, 13412, 28820, 11296, 16490, 1211, 10632, 497, 7869, 82, 6055, 1211, 2655, 9957, 11296, 20903, 45841, 13496, 6055, 1211, 2655, 11296, 9701, 10744, 260, 4032, 4724, 1829, 9381, 13546, 11331, 7435, 2304, 11296, 3714, 4587, 1211, 38688, 5296, 28814, 20292, 23032, 3555, 3615, 995, 20666, 1863, 35457, 3660, 24976, 4477, 3390, 2815, 43840, 9122, 33911, 41850, 4724, 38436, 2304, 2423, 2744, 4032, 2423, 20903, 45841, 13496, 2701, 9154, 16490, 1211, 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mathematical formula صياغ رياضية تمام استخدم فيها أو تم استخدام فيها ال hematocrit و ال hemoglobin level و ال RBCs إذا تم توظيف ال hematocrit ال hemoglobin level و أيضا ال RBCs count لحتى", "tokens": [5016, 3794, 16758, 9307, 9154, 16490, 1211, 6027, 18894, 8513, 20328, 1829, 5718, 118, 12602, 1829, 46958, 10632, 46811, 10943, 44713, 9778, 40448, 8978, 11296, 34051, 46811, 44713, 9778, 3215, 10943, 8978, 11296, 2423, 8636, 267, 3617, 270, 4032, 2423, 8636, 45841, 13496, 1496, 4032, 2423, 497, 7869, 82, 11933, 15730, 46811, 6055, 2407, 19913, 33911, 2423, 8636, 267, 3617, 270, 2423, 8636, 45841, 13496, 1496, 4032, 36632, 11242, 995, 2423, 497, 7869, 82, 1207, 5296, 33753, 7578], "avg_logprob": -0.1766218373292609, "compression_ratio": 1.615819209039548, "no_speech_prob": 3.039836883544922e-06, "words": [{"start": 171.95, "end": 173.01, "word": "حسابات", "probability": 0.8707275390625}, {"start": 173.01, "end": 173.17, "word": " من", "probability": 0.98779296875}, {"start": 173.17, 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و ال mean corpuscular homoglobin concentration mcv mch mchc طبعا طيب ال mean متوسط معناها corpuscular يعني يعني بتعود على كلمة كرة لإن احنا بنحكي عن خلايا الدم الحمراء كريات الدم الحمراء و كريات الدم البيضاء طبعا فإذا corpuscular يعني جاية من .. من .. من ..", "tokens": [2407, 2423, 275, 66, 85, 4032, 2423, 914, 1181, 31624, 17792, 3655, 45841, 13496, 4032, 2423, 914, 1181, 31624, 17792, 3655, 45841, 13496, 9856, 275, 66, 85, 275, 339, 275, 339, 66, 23032, 3555, 3615, 995, 23032, 1829, 3555, 2423, 914, 44650, 41779, 9566, 20449, 8315, 11296, 1181, 31624, 17792, 37495, 22653, 37495, 22653, 39894, 3615, 23328, 15844, 9122, 19528, 3660, 9122, 25720, 5296, 28814, 1863, 1975, 5016, 8315, 44945, 5016, 4117, 1829, 18871, 16490, 15040, 25528, 32748, 2304, 21542, 29973, 16606, 9122, 16572, 9307, 32748, 2304, 21542, 29973, 16606, 4032, 9122, 16572, 9307, 32748, 2304, 2423, 21292, 11242, 16606, 23032, 3555, 3615, 995, 6156, 28814, 15730, 1181, 31624, 17792, 37495, 22653, 10874, 995, 10632, 9154, 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"word": " معناها", "probability": 0.9586588541666666}, {"start": 275.77, "end": 276.63, "word": " corpuscular", "probability": 0.8995768229166666}, {"start": 276.63, "end": 277.47, "word": " يعني", "probability": 0.922119140625}, {"start": 277.47, "end": 278.49, "word": " يعني", "probability": 0.761474609375}, {"start": 278.49, "end": 279.61, "word": " بتعود", "probability": 0.92138671875}, {"start": 279.61, "end": 279.89, "word": " على", "probability": 0.81591796875}, {"start": 279.89, "end": 280.43, "word": " كلمة", "probability": 0.9886067708333334}, {"start": 280.43, "end": 280.81, "word": " كرة", "probability": 0.9365234375}, {"start": 280.81, "end": 281.17, "word": " لإن", "probability": 0.9514973958333334}, {"start": 281.17, "end": 281.45, "word": " احنا", "probability": 0.79345703125}, {"start": 281.45, "end": 282.29, "word": " بنحكي", "probability": 0.92626953125}, {"start": 282.29, "end": 282.45, "word": " عن", "probability": 0.99072265625}, {"start": 282.45, "end": 282.89, "word": " خلايا", "probability": 0.962890625}, {"start": 282.89, "end": 283.27, "word": " الدم", "probability": 0.864990234375}, {"start": 283.27, "end": 284.29, "word": " الحمراء", "probability": 0.93701171875}, {"start": 284.29, "end": 284.99, "word": " كريات", "probability": 0.876953125}, {"start": 284.99, "end": 285.31, "word": " الدم", "probability": 0.975830078125}, {"start": 285.31, "end": 285.83, "word": " الحمراء", "probability": 0.9793294270833334}, {"start": 285.83, "end": 286.01, "word": " و", "probability": 0.99072265625}, {"start": 286.01, "end": 286.53, "word": " كريات", "probability": 0.98486328125}, {"start": 286.53, "end": 286.79, "word": " الدم", "probability": 0.98681640625}, {"start": 286.79, "end": 287.35, "word": " البيضاء", "probability": 0.846435546875}, {"start": 287.35, "end": 287.99, "word": " طبعا", "probability": 0.9532470703125}, {"start": 287.99, "end": 288.59, "word": " فإذا", "probability": 0.7307942708333334}, {"start": 288.59, "end": 289.33, "word": " corpuscular", "probability": 0.85888671875}, {"start": 289.33, "end": 289.69, "word": " يعني", "probability": 0.976318359375}, {"start": 289.69, "end": 290.01, "word": " جاية", "probability": 0.8860677083333334}, {"start": 290.01, "end": 290.19, "word": " من", "probability": 0.9921875}, {"start": 290.19, "end": 290.33, "word": " ..", "probability": 0.53955078125}, {"start": 290.33, "end": 290.47, "word": " من", "probability": 0.93603515625}, {"start": 290.47, "end": 290.95, "word": " ..", "probability": 0.94091796875}, {"start": 290.95, "end": 290.95, "word": " من", "probability": 0.96630859375}, {"start": 290.95, "end": 290.95, "word": " ..", "probability": 0.890625}], "temperature": 1.0}, {"id": 13, "seek": 32029, "start": 290.99, "end": 320.29, "text": "من شيء كراوي أي يعبّر عن الخلية، تمام؟ يعني هو متوسط، اذا mean carboscular volume هاي .. هاي Syria معناها متوسط حجم الخلية، mean carboscular homoglobin متوسط حجم ال .. او متوسط سوري ال homoglobin content ل الخلية او الكريه تمام؟ و ال mean carboscular homoglobin concentration متوسط تركيز ال homoglobin في الكريه او في الخلية", "tokens": [27842, 44049, 38207, 9122, 23557, 45865, 36632, 7251, 3615, 3555, 11703, 2288, 18871, 33962, 1211, 10632, 12399, 46811, 10943, 22807, 37495, 22653, 31439, 44650, 41779, 9566, 12399, 1975, 15730, 914, 12143, 329, 17792, 5523, 8032, 47302, 4386, 8032, 47302, 13314, 20449, 8315, 11296, 44650, 41779, 9566, 11331, 7435, 2304, 33962, 1211, 10632, 12399, 914, 12143, 329, 17792, 3655, 45841, 13496, 44650, 41779, 9566, 11331, 7435, 2304, 2423, 4386, 1975, 2407, 44650, 41779, 9566, 8608, 13063, 1829, 2423, 3655, 45841, 13496, 2701, 5296, 33962, 1211, 10632, 1975, 2407, 33251, 16572, 3224, 46811, 10943, 22807, 4032, 2423, 914, 12143, 329, 17792, 3655, 45841, 13496, 9856, 44650, 41779, 9566, 6055, 31747, 1829, 11622, 2423, 3655, 45841, 13496, 8978, 33251, 16572, 3224, 1975, 2407, 8978, 33962, 1211, 10632], "avg_logprob": -0.20875000047683717, "compression_ratio": 1.9957805907172996, 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"probability": 0.82275390625}, {"start": 303.09, "end": 303.77, "word": " carboscular", "probability": 0.7972005208333334}, {"start": 303.77, "end": 304.49, "word": " homoglobin", "probability": 0.8605143229166666}, {"start": 304.49, "end": 305.31, "word": " متوسط", "probability": 0.97509765625}, {"start": 305.31, "end": 306.17, "word": " حجم", "probability": 0.99072265625}, {"start": 306.17, "end": 306.57, "word": " ال", "probability": 0.9638671875}, {"start": 306.57, "end": 306.83, "word": " ..", "probability": 0.95654296875}, {"start": 306.83, "end": 307.09, "word": " او", "probability": 0.8857421875}, {"start": 307.09, "end": 307.57, "word": " متوسط", "probability": 0.9886067708333334}, {"start": 307.57, "end": 307.89, "word": " سوري", "probability": 0.7874348958333334}, {"start": 307.89, "end": 308.09, "word": " ال", "probability": 0.9365234375}, {"start": 308.09, "end": 308.71, "word": " homoglobin", "probability": 0.7857259114583334}, {"start": 308.71, "end": 309.33, "word": " content", "probability": 0.97509765625}, {"start": 309.33, "end": 309.81, "word": " ل", "probability": 0.8564453125}, {"start": 309.81, "end": 310.57, "word": " الخلية", "probability": 0.8577473958333334}, {"start": 310.57, "end": 310.93, "word": " او", "probability": 0.7783203125}, {"start": 310.93, "end": 312.05, "word": " الكريه", "probability": 0.5437825520833334}, {"start": 312.05, "end": 313.09, "word": " تمام؟", "probability": 0.8590494791666666}, {"start": 313.09, "end": 313.35, "word": " و", "probability": 0.93359375}, {"start": 313.35, "end": 313.69, "word": " ال", "probability": 0.90673828125}, {"start": 313.69, "end": 313.91, "word": " mean", "probability": 0.7802734375}, {"start": 313.91, "end": 314.61, "word": " carboscular", "probability": 0.724365234375}, {"start": 314.61, "end": 315.13, "word": " homoglobin", "probability": 0.9278971354166666}, {"start": 315.13, "end": 316.03, "word": " concentration", "probability": 0.92041015625}, {"start": 316.03, "end": 317.07, "word": " متوسط", "probability": 0.9806315104166666}, {"start": 317.07, "end": 317.75, "word": " تركيز", "probability": 0.967529296875}, {"start": 317.75, "end": 317.89, "word": " ال", "probability": 0.974609375}, {"start": 317.89, "end": 318.47, "word": " homoglobin", "probability": 0.9254557291666666}, {"start": 318.47, "end": 318.69, "word": " في", "probability": 0.97216796875}, {"start": 318.69, "end": 319.33, "word": " الكريه", "probability": 0.98779296875}, {"start": 319.33, "end": 319.59, "word": " او", "probability": 0.885986328125}, {"start": 319.59, "end": 319.75, "word": " في", "probability": 0.87109375}, {"start": 319.75, "end": 320.29, "word": " الخلية", "probability": 0.9781901041666666}], "temperature": 1.0}, {"id": 14, "seek": 34798, "start": 320.64, "end": 347.98, "text": "تمام؟ اذا MCV, MCH, MCHC هدول هم ال indices هدول هم المؤشرات و القياسات اللي لهم صياغ و معادلات حسابية باستخدام ال hemotocrit و ال homoglobin level و RBC count انا ممكن احسبهم تمام؟ و بيدللولي على حجم الخلية و على محتوها من ال homoglobin و هذا بساعدني بتشخيص القنينة تمام؟", "tokens": [39237, 10943, 22807, 1975, 15730, 8797, 53, 11, 376, 5462, 11, 376, 5462, 34, 8032, 3215, 12610, 8032, 2304, 2423, 43840, 8032, 3215, 12610, 8032, 2304, 9673, 33604, 46309, 9307, 4032, 25062, 25528, 3794, 9307, 13672, 1829, 5296, 16095, 20328, 1829, 5718, 118, 4032, 20449, 18513, 1211, 9307, 11331, 3794, 16758, 10632, 4724, 995, 14851, 9778, 3215, 10943, 2423, 8636, 310, 905, 3210, 4032, 2423, 3655, 45841, 13496, 1496, 4032, 497, 7869, 1207, 1975, 8315, 3714, 43020, 1975, 5016, 35457, 16095, 46811, 10943, 22807, 4032, 4724, 25708, 1211, 1211, 12610, 1829, 15844, 11331, 7435, 2304, 33962, 1211, 10632, 4032, 15844, 3714, 33753, 2407, 11296, 9154, 2423, 3655, 45841, 13496, 4032, 23758, 4724, 3794, 995, 22488, 22653, 39894, 8592, 9778, 1829, 9381, 25062, 1863, 9957, 3660, 46811, 10943, 22807], "avg_logprob": -0.1711482558139535, "compression_ratio": 1.6875, "no_speech_prob": 4.029273986816406e-05, 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"word": " مثال", "probability": 0.9912109375}, {"start": 1133.17, "end": 1133.61, "word": " عليها", "probability": 0.96044921875}, {"start": 1133.61, "end": 1133.83, "word": " ال", "probability": 0.92822265625}, {"start": 1133.83, "end": 1134.21, "word": " iron", "probability": 0.8330078125}, {"start": 1134.21, "end": 1134.83, "word": " deficiency", "probability": 0.95703125}, {"start": 1134.83, "end": 1135.43, "word": " أنيميا", "probability": 0.735595703125}, {"start": 1135.43, "end": 1135.93, "word": " و", "probability": 0.701171875}, {"start": 1135.93, "end": 1136.87, "word": " السلسيميا", "probability": 0.730078125}, {"start": 1136.87, "end": 1137.51, "word": " تمام", "probability": 0.9814453125}, {"start": 1137.51, "end": 1137.99, "word": " ال", "probability": 0.6171875}, {"start": 1137.99, "end": 1138.29, "word": " iron", "probability": 0.9658203125}, {"start": 1138.29, "end": 1138.79, "word": " deficiency", "probability": 0.99267578125}, {"start": 1138.79, "end": 1139.29, "word": " أنيميا", "probability": 0.9573567708333334}, {"start": 1139.29, "end": 1139.45, "word": " اللي", "probability": 0.894287109375}, {"start": 1139.45, "end": 1139.59, "word": " هي", "probability": 0.85888671875}, {"start": 1139.59, "end": 1139.97, "word": " أنيميا", "probability": 0.9503580729166666}, {"start": 1139.97, "end": 1140.27, "word": " نقص", "probability": 0.97705078125}, {"start": 1140.27, "end": 1140.89, "word": " الحديد", "probability": 0.95703125}, {"start": 1140.89, "end": 1141.19, "word": " و", "probability": 0.765625}, {"start": 1141.19, "end": 1142.09, "word": " السلسيميا", "probability": 0.913671875}, {"start": 1142.09, "end": 1142.67, "word": " اللي", "probability": 0.974609375}, {"start": 1142.67, "end": 1143.01, "word": " هي", "probability": 0.96533203125}, {"start": 1143.01, "end": 1143.49, "word": " ال", "probability": 0.89794921875}, {"start": 1143.49, "end": 1143.69, "word": " ..", "probability": 0.921875}], "temperature": 1.0}, {"id": 51, "seek": 116163, "start": 1144.59, "end": 1161.63, "text": "Anemia البحر الأبيض المتوسط هدول التنتين بنسميهم microcytic الخلايا بتكون فيها صغيرة و ال homoglobin content فيها قليل تمام ال homoglobin فيها قليل ليش؟ لإن هنا ماعنديش iron كفاية", "tokens": [7828, 14058, 29739, 5016, 2288, 16247, 21292, 11242, 9673, 2655, 41779, 9566, 8032, 3215, 12610, 16712, 29399, 9957, 44945, 38251, 1829, 16095, 4532, 1344, 40907, 33962, 15040, 25528, 39894, 30544, 8978, 11296, 20328, 17082, 48923, 4032, 2423, 3655, 45841, 13496, 2701, 8978, 11296, 12174, 20292, 1211, 46811, 10943, 2423, 3655, 45841, 13496, 8978, 11296, 12174, 20292, 1211, 32239, 8592, 22807, 5296, 28814, 1863, 34105, 19446, 3615, 1863, 16254, 8592, 6497, 9122, 5172, 995, 10632], "avg_logprob": -0.12468750516573589, "compression_ratio": 1.521505376344086, "no_speech_prob": 1.4781951904296875e-05, "words": [{"start": 1144.59, "end": 1145.77, "word": "Anemia", "probability": 0.677734375}, {"start": 1145.77, "end": 1146.99, "word": " البحر", "probability": 0.91943359375}, {"start": 1146.99, "end": 1147.31, "word": " الأبيض", "probability": 0.9734700520833334}, {"start": 1147.31, "end": 1148.01, "word": " المتوسط", "probability": 0.9937744140625}, {"start": 1148.01, "end": 1148.71, "word": " هدول", "probability": 0.8828125}, {"start": 1148.71, "end": 1149.31, "word": " التنتين", "probability": 0.7373860677083334}, {"start": 1149.31, "end": 1149.83, "word": " بنسميهم", "probability": 0.9033203125}, {"start": 1149.83, "end": 1150.57, "word": " microcytic", "probability": 0.759765625}, {"start": 1150.57, "end": 1151.13, "word": " الخلايا", "probability": 0.9402669270833334}, {"start": 1151.13, "end": 1151.69, "word": " بتكون", "probability": 0.979736328125}, {"start": 1151.69, "end": 1152.25, "word": " فيها", "probability": 0.958251953125}, {"start": 1152.25, "end": 1153.33, "word": " صغيرة", "probability": 0.9910481770833334}, {"start": 1153.33, "end": 1153.77, "word": " و", "probability": 0.8359375}, {"start": 1153.77, "end": 1153.89, "word": " ال", "probability": 0.9150390625}, {"start": 1153.89, "end": 1154.35, "word": " homoglobin", "probability": 0.8483072916666666}, {"start": 1154.35, "end": 1154.77, "word": " content", "probability": 0.96044921875}, {"start": 1154.77, "end": 1155.25, "word": " فيها", "probability": 0.968505859375}, {"start": 1155.25, "end": 1156.01, "word": " قليل", "probability": 0.9930013020833334}, {"start": 1156.01, "end": 1156.65, "word": " تمام", "probability": 0.787353515625}, {"start": 1156.65, "end": 1156.83, "word": " ال", "probability": 0.69384765625}, {"start": 1156.83, "end": 1157.33, "word": " homoglobin", "probability": 0.88037109375}, {"start": 1157.33, "end": 1157.65, "word": " فيها", "probability": 0.981201171875}, {"start": 1157.65, "end": 1158.39, "word": " قليل", "probability": 0.99365234375}, {"start": 1158.39, "end": 1159.17, "word": " ليش؟", "probability": 0.75048828125}, {"start": 1159.17, "end": 1159.51, "word": " لإن", "probability": 0.91748046875}, {"start": 1159.51, "end": 1159.87, "word": " هنا", "probability": 0.76025390625}, {"start": 1159.87, "end": 1160.69, "word": " ماعنديش", "probability": 0.9552734375}, {"start": 1160.69, "end": 1161.07, "word": " iron", "probability": 0.6123046875}, {"start": 1161.07, "end": 1161.63, "word": " كفاية", "probability": 0.9774169921875}], "temperature": 1.0}, {"id": 52, "seek": 118742, "start": 1161.84, "end": 1187.42, "text": "فال .. ال homoglobin اللي بيتصنع ما بيكونش ما .. إله iron فبالتالي بيكون فيه نقص في ال .. ال iron فبت .. في ال homoglobin كمان و نقص في ال oxygen delivery الثلاثيمية هي طفرة في جينات ال .. ال homoglobin فبيتم تصنيع .. بيصير فيه decrease production من ال homoglobin", "tokens": [5172, 6027, 4386, 2423, 3655, 45841, 13496, 13672, 1829, 4724, 36081, 9381, 1863, 3615, 19446, 4724, 1829, 30544, 8592, 19446, 4386, 11933, 43761, 6497, 6156, 3555, 6027, 2655, 6027, 1829, 4724, 1829, 30544, 8978, 3224, 8717, 4587, 9381, 8978, 2423, 4386, 2423, 6497, 6156, 3555, 2655, 4386, 8978, 2423, 3655, 45841, 13496, 9122, 2304, 7649, 4032, 8717, 4587, 9381, 8978, 2423, 9169, 8982, 6024, 104, 1211, 5718, 104, 32640, 10632, 39896, 23032, 5172, 25720, 8978, 10874, 1829, 8315, 2655, 2423, 4386, 2423, 3655, 45841, 13496, 6156, 21292, 39237, 6055, 9381, 22653, 3615, 4386, 4724, 1829, 9381, 13546, 8978, 3224, 11514, 4265, 9154, 2423, 3655, 45841, 13496], "avg_logprob": -0.14120911298511185, "compression_ratio": 1.8509615384615385, "no_speech_prob": 1.7881393432617188e-07, "words": [{"start": 1161.84, "end": 1162.66, "word": "فال", "probability": 0.787109375}, {"start": 1162.66, "end": 1162.66, "word": " ..", "probability": 0.62353515625}, {"start": 1162.66, "end": 1162.96, "word": " ال", "probability": 0.966796875}, {"start": 1162.96, "end": 1163.44, "word": " homoglobin", "probability": 0.7648111979166666}, {"start": 1163.44, "end": 1163.58, "word": " اللي", "probability": 0.8037109375}, {"start": 1163.58, "end": 1164.28, "word": " بيتصنع", "probability": 0.9771484375}, {"start": 1164.28, "end": 1164.48, "word": " ما", "probability": 0.8037109375}, {"start": 1164.48, "end": 1165.0, "word": " بيكونش", "probability": 0.87939453125}, {"start": 1165.0, "end": 1165.52, "word": " ما", "probability": 0.477294921875}, {"start": 1165.52, "end": 1165.7, "word": " ..", "probability": 0.6845703125}, {"start": 1165.7, "end": 1165.9, "word": " إله", "probability": 0.51904296875}, {"start": 1165.9, "end": 1166.4, "word": " iron", "probability": 0.86328125}, {"start": 1166.4, "end": 1167.36, "word": " فبالتالي", "probability": 0.9752604166666666}, {"start": 1167.36, "end": 1168.06, "word": " بيكون", "probability": 0.97998046875}, {"start": 1168.06, "end": 1168.36, "word": " فيه", "probability": 0.88916015625}, {"start": 1168.36, "end": 1168.68, "word": " نقص", "probability": 0.9871419270833334}, {"start": 1168.68, "end": 1168.96, "word": " في", "probability": 0.99169921875}, {"start": 1168.96, "end": 1170.26, "word": " ال", "probability": 0.97998046875}, {"start": 1170.26, "end": 1170.26, "word": " ..", "probability": 0.83154296875}, {"start": 1170.26, "end": 1170.76, "word": " ال", "probability": 0.97802734375}, {"start": 1170.76, "end": 1171.18, "word": " iron", "probability": 0.9521484375}, {"start": 1171.18, "end": 1171.6, "word": " فبت", "probability": 0.810546875}, {"start": 1171.6, "end": 1171.84, "word": " ..", "probability": 0.1591796875}, {"start": 1171.84, "end": 1172.14, "word": " في", "probability": 0.6767578125}, {"start": 1172.14, "end": 1172.6, "word": " ال", "probability": 0.943359375}, {"start": 1172.6, "end": 1173.36, "word": " homoglobin", "probability": 0.9215494791666666}, {"start": 1173.36, "end": 1173.86, "word": " كمان", "probability": 0.9973958333333334}, {"start": 1173.86, "end": 1174.38, "word": " و", "probability": 0.98486328125}, {"start": 1174.38, "end": 1175.42, "word": " نقص", "probability": 0.9833984375}, {"start": 1175.42, "end": 1175.62, "word": " في", "probability": 0.99560546875}, {"start": 1175.62, "end": 1175.82, "word": " ال", "probability": 0.99169921875}, {"start": 1175.82, "end": 1176.16, "word": " oxygen", "probability": 0.94384765625}, {"start": 1176.16, "end": 1176.76, "word": " delivery", "probability": 0.9345703125}, {"start": 1176.76, "end": 1178.06, "word": " الثلاثيمية", "probability": 0.8472377232142857}, {"start": 1178.06, "end": 1178.6, "word": " هي", "probability": 0.919921875}, {"start": 1178.6, "end": 1179.04, "word": " طفرة", "probability": 0.8761393229166666}, {"start": 1179.04, "end": 1179.22, "word": " في", "probability": 0.9912109375}, {"start": 1179.22, "end": 1180.18, "word": " جينات", "probability": 0.9625244140625}, {"start": 1180.18, "end": 1180.64, "word": " ال", "probability": 0.99365234375}, {"start": 1180.64, "end": 1181.18, "word": " ..", "probability": 0.9775390625}, {"start": 1181.18, "end": 1181.52, "word": " ال", "probability": 0.9755859375}, {"start": 1181.52, "end": 1182.06, "word": " homoglobin", "probability": 0.9588216145833334}, {"start": 1182.06, "end": 1183.18, "word": " فبيتم", "probability": 0.9749348958333334}, {"start": 1183.18, "end": 1183.84, "word": " تصنيع", "probability": 0.9078369140625}, {"start": 1183.84, "end": 1183.88, "word": " ..", "probability": 0.77490234375}, {"start": 1183.88, "end": 1184.14, "word": " بيصير", "probability": 0.9722900390625}, {"start": 1184.14, "end": 1184.34, "word": " فيه", "probability": 0.71630859375}, {"start": 1184.34, "end": 1184.94, "word": " decrease", "probability": 0.978515625}, {"start": 1184.94, "end": 1186.22, "word": " production", "probability": 0.796875}, {"start": 1186.22, "end": 1186.68, "word": " من", "probability": 0.99755859375}, {"start": 1186.68, "end": 1186.88, "word": " ال", "probability": 0.97021484375}, {"start": 1186.88, "end": 1187.42, "word": " homoglobin", "probability": 0.9567057291666666}], "temperature": 1.0}, {"id": 53, "seek": 121469, "start": 1189.31, "end": 1214.69, "text": "أو ممكن يكون الطفرة أعطتني هموجلوبين الشكل تبعه و التركيب تبعه abnormal فبالتالي الوظيفة تبعته هتكون abnormal ما بتحمل يعني أكسجين طيب ال macrocytic anemia أو ال macrocytic cells طيب أنيميا نتجت عن خلايا كبيرة في الحجم هذه بنشوفها في liver disease وفي الhypothyroidism تمام يعني", "tokens": [10721, 2407, 3714, 43020, 7251, 30544, 41950, 5172, 25720, 5551, 3615, 9566, 2655, 22653, 8032, 2304, 29245, 1211, 37746, 9957, 25124, 28820, 6055, 3555, 3615, 3224, 4032, 16712, 31747, 1829, 3555, 6055, 3555, 3615, 3224, 32847, 6156, 3555, 6027, 2655, 6027, 1829, 2423, 2407, 19913, 33911, 3660, 6055, 3555, 34268, 3224, 8032, 2655, 30544, 32847, 19446, 39894, 35571, 1211, 37495, 22653, 5551, 4117, 3794, 7435, 9957, 23032, 1829, 3555, 2423, 18887, 1344, 40907, 364, 14058, 34051, 2423, 18887, 1344, 40907, 5438, 23032, 1829, 3555, 14739, 32640, 25528, 8717, 2655, 7435, 2655, 18871, 16490, 15040, 25528, 9122, 3555, 48923, 8978, 21542, 7435, 2304, 29538, 44945, 8592, 38688, 11296, 8978, 15019, 4752, 4032, 41185, 2423, 3495, 79, 18907, 6490, 1434, 46811, 10943, 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"end": 1723.62, "text": "طيب ال mean corpuscular homoglobin متوسط ال homoglobin في الكورية طبعا؟ طب متوسط ال homoglobin ايش؟ وزن ال homoglobin طبعا؟ the average weight of homoglobin in an RBCs طيب وحدة القياس ال picogram طبعا؟ ال picogram و ال picogram هو عبارة عن عشرة أسالب اتناش ملي جرام طيب", "tokens": [9566, 1829, 3555, 2423, 914, 1181, 31624, 17792, 3655, 45841, 13496, 44650, 41779, 9566, 2423, 3655, 45841, 13496, 8978, 33251, 13063, 10632, 23032, 3555, 3615, 995, 22807, 23032, 3555, 44650, 41779, 9566, 2423, 3655, 45841, 13496, 1975, 1829, 8592, 22807, 4032, 11622, 1863, 2423, 3655, 45841, 13496, 23032, 3555, 3615, 995, 22807, 264, 4274, 3364, 295, 3655, 45841, 13496, 294, 364, 497, 7869, 82, 23032, 1829, 3555, 4032, 24401, 3660, 25062, 25528, 3794, 2423, 13363, 12820, 23032, 3555, 3615, 995, 22807, 2423, 13363, 12820, 4032, 2423, 13363, 12820, 31439, 6225, 3555, 9640, 3660, 18871, 6225, 8592, 25720, 5551, 3794, 6027, 3555, 1975, 2655, 1863, 33599, 3714, 20292, 10874, 2288, 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بتحصرلي أنواع معينة مثلا normocytic-normochromic في عندي أنواع معينة واحد اتنين تلاتة فبضل .. خلاص بنحصر بي هدول الأنواع ببدأ أعمل احوصات تانية تفرق ما بين الأنواع تبعوني normocytic-normochromic تمام؟ كانت مؤشرات وشكل ال .. ال RBCs", "tokens": [10721, 2407, 9381, 5172, 8717, 45367, 16247, 1863, 32640, 25528, 6156, 3555, 6027, 2655, 6027, 1829, 39894, 5016, 9381, 2288, 20292, 14739, 14407, 3615, 20449, 9957, 3660, 50113, 15040, 2026, 905, 43658, 12, 13403, 8997, 4397, 299, 8978, 18871, 16254, 14739, 14407, 3615, 20449, 9957, 3660, 36764, 24401, 1975, 2655, 1863, 9957, 6055, 1211, 9307, 3660, 6156, 3555, 11242, 1211, 4386, 16490, 1211, 33546, 44945, 5016, 9381, 2288, 4724, 1829, 8032, 3215, 12610, 16247, 1863, 14407, 3615, 4724, 44510, 10721, 5551, 25957, 1211, 1975, 5016, 2407, 9381, 9307, 6055, 7649, 10632, 6055, 5172, 2288, 4587, 19446, 49374, 16247, 1863, 14407, 3615, 6055, 3555, 3615, 11536, 1829, 2026, 905, 43658, 12, 13403, 8997, 4397, 299, 46811, 10943, 22807, 25961, 2655, 3714, 33604, 46309, 9307, 4032, 8592, 28820, 2423, 4386, 2423, 497, 7869, 82], "avg_logprob": -0.17316729816279017, "compression_ratio": 1.8189655172413792, "no_speech_prob": 2.7418136596679688e-06, "words": [{"start": 2332.46, "end": 2333.42, "word": "أوصف", "probability": 0.9227294921875}, {"start": 2333.42, "end": 2334.26, "word": " نوع", "probability": 0.78759765625}, {"start": 2334.26, "end": 2335.0, "word": " الأنيميا", "probability": 0.7337646484375}, {"start": 2335.0, "end": 2335.94, "word": " فبالتالي", "probability": 0.978515625}, {"start": 2335.94, "end": 2337.14, "word": " بتحصرلي", "probability": 0.8568359375}, {"start": 2337.14, "end": 2337.48, "word": " أنواع", "probability": 0.94287109375}, {"start": 2337.48, "end": 2337.98, "word": " معينة", "probability": 0.9915364583333334}, {"start": 2337.98, "end": 2338.22, "word": " مثلا", "probability": 0.95751953125}, {"start": 2338.22, "end": 2338.86, "word": " normocytic", "probability": 0.666015625}, {"start": 2338.86, "end": 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اذا انا هتوجه اما هتكون ثلاثيميا او iron deficiency anemia ممكن اعمل فحوصات لمستوى ال iron ممكن يعني اعمل فحوصات لحتى اكشف عن جيناتي ال homoglobin و اشوف اذا فيها خلل عشان اشخص ثلاثيميا يعني فبالتالي اكون محصورة بالانواع اللي هي microcytic hypochromic", "tokens": [2407, 2423, 43840, 13672, 1829, 1975, 8315, 11331, 35457, 2655, 16095, 4724, 1829, 5016, 4117, 12610, 1829, 16472, 11296, 4532, 1344, 40907, 2477, 2259, 339, 4397, 299, 1975, 15730, 1975, 8315, 8032, 2655, 29245, 3224, 1975, 15042, 8032, 2655, 30544, 38637, 1211, 5718, 104, 32640, 25528, 1975, 2407, 6497, 37500, 364, 14058, 3714, 43020, 1975, 25957, 1211, 6156, 5016, 2407, 9381, 9307, 32767, 14851, 2407, 7578, 2423, 6497, 3714, 43020, 37495, 22653, 1975, 25957, 1211, 6156, 5016, 2407, 9381, 9307, 5296, 33753, 7578, 1975, 4117, 8592, 5172, 18871, 10874, 1829, 8315, 31371, 2423, 3655, 45841, 13496, 4032, 1975, 8592, 38688, 1975, 15730, 8978, 11296, 16490, 1211, 1211, 6225, 8592, 7649, 1975, 8592, 9778, 9381, 38637, 1211, 5718, 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حكينا إنها بتساعدني في وصف نوع الأنيميا و ال classification و ال differential diagnosis التفريق ما بين هاي الأنيميات", "tokens": [9566, 2304, 10943, 22807, 18887, 1344, 66, 1050, 12, 13403, 8997, 4397, 299, 4724, 43042, 3224, 9122, 2304, 7649, 8717, 36178, 16247, 8592, 1829, 16606, 6156, 3555, 6027, 2655, 6027, 1829, 11933, 15730, 11331, 4117, 1829, 8315, 36145, 11296, 39894, 3794, 995, 22488, 22653, 8978, 4032, 9381, 5172, 8717, 45367, 16247, 22653, 2304, 25528, 4032, 2423, 21538, 4032, 2423, 15756, 15217, 16712, 5172, 16572, 4587, 19446, 49374, 8032, 47302, 16247, 22653, 2304, 1829, 9307], "avg_logprob": -0.19427083929379782, "compression_ratio": 1.4381443298969072, "no_speech_prob": 5.960464477539063e-08, "words": [{"start": 2385.06, "end": 2386.14, "word": "طمام؟", "probability": 0.57122802734375}, {"start": 2386.14, "end": 2386.82, "word": " macrocyclic", "probability": 0.6551513671875}, {"start": 2386.82, "end": 2387.4, "word": "-normochromic", "probability": 0.772998046875}, 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"probability": 0.83349609375}, {"start": 2650.59, "end": 2651.03, "word": " ال", "probability": 0.8935546875}, {"start": 2651.03, "end": 2651.51, "word": " MCV", "probability": 0.83154296875}, {"start": 2651.51, "end": 2651.91, "word": " تبعها", "probability": 0.9315185546875}, {"start": 2651.91, "end": 2652.29, "word": " هيكون", "probability": 0.9541015625}, {"start": 2652.29, "end": 2652.91, "word": " قليل", "probability": 0.9918619791666666}, {"start": 2652.91, "end": 2654.33, "word": " normochromic", "probability": 0.84112548828125}, {"start": 2654.33, "end": 2654.73, "word": " خلية", "probability": 0.96435546875}, {"start": 2654.73, "end": 2655.09, "word": " اللون", "probability": 0.989990234375}, {"start": 2655.09, "end": 2655.39, "word": " فيها", "probability": 0.98046875}, {"start": 2655.39, "end": 2655.99, "word": " طبيعي", "probability": 0.9561767578125}, {"start": 2655.99, "end": 2656.49, "word": " إذا", "probability": 0.845947265625}, {"start": 2656.49, "end": 2656.75, "word": " ال", "probability": 0.5703125}, {"start": 2656.75, "end": 2656.97, "word": " red", "probability": 0.81103515625}, {"start": 2656.97, "end": 2657.29, "word": " blood", "probability": 0.9501953125}, {"start": 2657.29, "end": 2657.57, "word": " cell", "probability": 0.94189453125}, {"start": 2657.57, "end": 2657.95, "word": " عندها", "probability": 0.990966796875}, {"start": 2657.95, "end": 2658.39, "word": " normal", "probability": 0.88818359375}, {"start": 2658.39, "end": 2659.23, "word": " concentration", "probability": 0.8994140625}, {"start": 2659.23, "end": 2659.49, "word": " of", "probability": 0.98193359375}, {"start": 2659.49, "end": 2660.11, "word": " homoglobin", "probability": 0.6900227864583334}], "temperature": 1.0}, {"id": 117, "seek": 268877, "start": 2660.41, "end": 2688.77, "text": "normocytic يعني خلية حجمها طبيعي إذا ال red blood cell normal size ال MCV normal و ال red blood cell indices كشكل يعني كشكل عام إنها عبارة عن المؤشرات والقياسات اللي بتفسر الحجم و ال homoglobin content لل red blood cell تمام؟ و هذا هو التعريف اللي إحنا بدأنا في محاضرتنا عم نختم فيه هلأ و هيك بنكون وصلنا لنهاية المحاضرة يعطيكم ألف عافية", "tokens": [13403, 905, 43658, 37495, 22653, 16490, 1211, 10632, 11331, 7435, 2304, 11296, 23032, 21292, 3615, 1829, 11933, 15730, 2423, 2182, 3390, 2815, 2710, 2744, 2423, 8797, 53, 2710, 4032, 2423, 2182, 3390, 2815, 43840, 9122, 8592, 28820, 37495, 22653, 9122, 8592, 28820, 6225, 10943, 36145, 11296, 6225, 3555, 9640, 3660, 18871, 9673, 33604, 46309, 9307, 16070, 4587, 25528, 3794, 9307, 13672, 1829, 39894, 36178, 2288, 21542, 7435, 2304, 4032, 2423, 3655, 45841, 13496, 2701, 24976, 2182, 3390, 2815, 46811, 10943, 22807, 4032, 23758, 31439, 16712, 3615, 16572, 5172, 13672, 1829, 11933, 5016, 8315, 47525, 10721, 8315, 8978, 3714, 5016, 46958, 43500, 8315, 6225, 2304, 8717, 9778, 39237, 8978, 3224, 8032, 1211, 10721, 4032, 39896, 4117, 44945, 30544, 4032, 36520, 8315, 5296, 1863, 11296, 10632, 9673, 5016, 46958, 25720, 37495, 9566, 1829, 24793, 5551, 46538, 6225, 31845, 10632], "avg_logprob": -0.16191122648508652, "compression_ratio": 1.7575757575757576, "no_speech_prob": 0.0001876354217529297, "words": [{"start": 2660.41, "end": 2661.59, "word": "normocytic", "probability": 0.5697428385416666}, {"start": 2661.59, "end": 2662.05, "word": " يعني", "probability": 0.882568359375}, {"start": 2662.05, "end": 2662.57, "word": " خلية", "probability": 0.9627278645833334}, {"start": 2662.57, "end": 2663.11, "word": " حجمها", "probability": 0.9949951171875}, {"start": 2663.11, "end": 2663.67, "word": " طبيعي", "probability": 0.95361328125}, {"start": 2663.67, "end": 2664.11, "word": " إذا", "probability": 0.593505859375}, {"start": 2664.11, "end": 2664.31, "word": " ال", "probability": 0.6982421875}, {"start": 2664.31, "end": 2664.49, "word": " red", "probability": 0.33154296875}, {"start": 2664.49, "end": 2664.75, "word": " blood", "probability": 0.9228515625}, {"start": 2664.75, "end": 2665.07, "word": " cell", "probability": 0.84716796875}, {"start": 2665.07, "end": 2665.49, "word": " normal", "probability": 0.810546875}, {"start": 2665.49, "end": 2666.11, "word": " size", "probability": 0.8544921875}, {"start": 2666.11, "end": 2666.29, "word": " ال", "probability": 0.85546875}, {"start": 2666.29, "end": 2666.79, "word": " MCV", "probability": 0.843017578125}, {"start": 2666.79, "end": 2667.29, "word": " normal", "probability": 0.78759765625}, {"start": 2667.29, "end": 2667.99, "word": " و", "probability": 0.62548828125}, {"start": 2667.99, "end": 2668.25, "word": " ال", "probability": 0.84130859375}, {"start": 2668.25, "end": 2668.39, "word": " red", "probability": 0.83154296875}, {"start": 2668.39, "end": 2668.69, "word": " blood", "probability": 0.9462890625}, {"start": 2668.69, "end": 2668.95, "word": " cell", "probability": 0.86279296875}, {"start": 2668.95, "end": 2669.33, "word": " indices", "probability": 0.7763671875}, {"start": 2669.33, "end": 2670.01, "word": " كشكل", "probability": 0.8728841145833334}, {"start": 2670.01, "end": 2670.49, "word": " يعني", "probability": 0.915771484375}, {"start": 2670.49, "end": 2671.11, "word": " كشكل", "probability": 0.6865234375}, {"start": 2671.11, "end": 2671.57, "word": " عام", "probability": 0.983642578125}, {"start": 2671.57, "end": 2672.31, "word": " إنها", "probability": 0.73291015625}, {"start": 2672.31, "end": 2672.93, "word": " عبارة", "probability": 0.9261474609375}, {"start": 2672.93, "end": 2673.19, "word": " عن", "probability": 0.99658203125}, {"start": 2673.19, "end": 2674.11, "word": " المؤشرات", "probability": 0.960693359375}, {"start": 2674.11, "end": 2674.99, "word": " والقياسات", "probability": 0.82890625}, {"start": 2674.99, "end": 2675.25, "word": " اللي", "probability": 0.941162109375}, {"start": 2675.25, "end": 2675.83, "word": " بتفسر", "probability": 0.7692057291666666}, {"start": 2675.83, "end": 2676.65, "word": " الحجم", "probability": 0.9803059895833334}, {"start": 2676.65, "end": 2676.79, "word": " و", "probability": 0.87060546875}, {"start": 2676.79, "end": 2676.89, "word": " ال", "probability": 0.72607421875}, {"start": 2676.89, "end": 2677.39, "word": " homoglobin", "probability": 0.8561197916666666}, {"start": 2677.39, "end": 2677.81, "word": " content", "probability": 0.9833984375}, {"start": 2677.81, "end": 2678.03, "word": " لل", "probability": 0.6103515625}, {"start": 2678.03, "end": 2678.23, "word": " red", "probability": 0.71240234375}, {"start": 2678.23, "end": 2678.51, "word": " blood", "probability": 0.94775390625}, {"start": 2678.51, "end": 2678.89, "word": " cell", "probability": 0.87548828125}, {"start": 2678.89, "end": 2679.69, "word": " تمام؟", "probability": 0.8162434895833334}, {"start": 2679.69, "end": 2679.79, "word": " و", "probability": 0.61572265625}, {"start": 2679.79, "end": 2680.03, "word": " هذا", "probability": 0.85595703125}, {"start": 2680.03, "end": 2680.25, "word": " هو", "probability": 0.9873046875}, {"start": 2680.25, "end": 2680.75, "word": " التعريف", "probability": 0.9879150390625}, {"start": 2680.75, "end": 2680.91, "word": " اللي", "probability": 0.950439453125}, {"start": 2680.91, "end": 2681.35, "word": " إحنا", "probability": 0.778076171875}, {"start": 2681.35, "end": 2682.19, "word": " بدأنا", "probability": 0.9122721354166666}, {"start": 2682.19, "end": 2682.47, "word": " في", "probability": 0.9541015625}, {"start": 2682.47, "end": 2683.21, "word": " محاضرتنا", "probability": 0.96533203125}, {"start": 2683.21, "end": 2683.43, "word": " عم", "probability": 0.754638671875}, {"start": 2683.43, "end": 2683.77, "word": " نختم", "probability": 0.8816731770833334}, {"start": 2683.77, "end": 2684.01, "word": " فيه", "probability": 0.971435546875}, {"start": 2684.01, "end": 2684.37, "word": " هلأ", "probability": 0.8531901041666666}, {"start": 2684.37, "end": 2684.95, "word": " و", "probability": 0.76025390625}, {"start": 2684.95, "end": 2685.37, "word": " هيك", "probability": 0.96728515625}, {"start": 2685.37, "end": 2685.73, "word": " بنكون", "probability": 0.80908203125}, {"start": 2685.73, "end": 2686.09, "word": " وصلنا", "probability": 0.9729817708333334}, {"start": 2686.09, "end": 2686.57, "word": " لنهاية", "probability": 0.9334716796875}, {"start": 2686.57, "end": 2687.21, "word": " المحاضرة", "probability": 0.99609375}, {"start": 2687.21, "end": 2688.03, "word": " يعطيكم", "probability": 0.932373046875}, {"start": 2688.03, "end": 2688.35, "word": " ألف", "probability": 0.80126953125}, {"start": 2688.35, "end": 2688.77, "word": " عافية", "probability": 0.9501953125}], "temperature": 1.0}], "language": "ar", "language_probability": 1.0, "duration": 2694.42025, "duration_after_vad": 2659.6374999999935}
\ No newline at end of file
diff --git a/PL9fwy3NUQKwY9jl8xw-bWkVZkWcGGyspd/Up-Q0W10hks_raw.srt b/PL9fwy3NUQKwY9jl8xw-bWkVZkWcGGyspd/Up-Q0W10hks_raw.srt
new file mode 100644
index 0000000000000000000000000000000000000000..d606ec46e09a087680610842b15dc273a72cb3ec
--- /dev/null
+++ b/PL9fwy3NUQKwY9jl8xw-bWkVZkWcGGyspd/Up-Q0W10hks_raw.srt
@@ -0,0 +1,2352 @@
+1
+00:00:10,020 --> 00:00:13,720
+السلام عليكم ورحمة الله وبركاته طالبات العزيزات
+
+2
+00:00:13,720 --> 00:00:17,940
+اليوم ان شاء الله راح نتحدث عن red blood cell
+
+3
+00:00:17,940 --> 00:00:23,000
+indices مؤشرات خلايا الدم الحمراء أو معاملات خلايا
+
+4
+00:00:23,000 --> 00:00:26,760
+الدم الحمراء في البداية خلينا نعرف شو يعني red
+
+5
+00:00:26,760 --> 00:00:30,560
+blood cell indicesRed blood cell indices هي عبارة
+
+6
+00:00:30,560 --> 00:00:35,120
+عن measurement قياسات مؤشرات أو معاملات that
+
+7
+00:00:35,120 --> 00:00:39,840
+describe the size and oxygen carrying protein
+
+8
+00:00:39,840 --> 00:00:44,700
+content of red blood cell إذن هي معاملات ومؤشرات
+
+9
+00:00:44,700 --> 00:00:50,480
+بتصف حجم خلية ال RBCs بالإضافة إلى محتوها من ال
+
+10
+00:00:50,480 --> 00:00:53,620
+homoglobin اللي هو عبارة عن ال oxygen carrying
+
+11
+00:00:53,620 --> 00:00:57,240
+protein البروتين المسئول عن حملة ال oxygen ونقلة
+
+12
+00:00:57,740 --> 00:01:02,600
+طمام؟ إذا red blood cell indices هي مؤشرات تصف حجم
+
+13
+00:01:02,600 --> 00:01:07,400
+الخلية ومحتوها من ال homoglobin وإذا بتتذكروا لما
+
+14
+00:01:07,400 --> 00:01:11,960
+شرحنا محاضرة ال blood film حكينا إنه من خلال ال
+
+15
+00:01:11,960 --> 00:01:14,640
+blood film أو ال blood smear أنا بقيم ال
+
+16
+00:01:14,640 --> 00:01:19,220
+morphology ال shape للخليةوحكيتلكم بالنسبة لل RBCs
+
+17
+00:01:19,220 --> 00:01:24,200
+أنا بدرس .. بشوف حجمها بدرس أيضا محتوها من ال
+
+18
+00:01:24,200 --> 00:01:27,280
+homoglobin بشوفها خلايا فاضية أو فيها homoglobin
+
+19
+00:01:27,280 --> 00:01:30,600
+ال central baller اللي .. اللي هو شحوب المركز اللي
+
+20
+00:01:30,600 --> 00:01:35,100
+في داخلها هل يحتل طولتها او اكثر من ذلك وكل هذه
+
+21
+00:01:35,100 --> 00:01:40,640
+لها مؤشرات و مرتبطة بتشخيص ال anemia تمام؟ اذا ال
+
+22
+00:01:40,640 --> 00:01:44,440
+red blood cell indices ايضا هي عبارة عن عوامل
+
+23
+00:01:44,440 --> 00:01:51,600
+ومؤشراتيعني حسابات قياسات أيضا بتدلليلي عن حجم
+
+24
+00:01:51,600 --> 00:01:56,480
+الخلية ومحتوها من ال homoglobin تمام؟ وبالتالي إذا
+
+25
+00:01:56,480 --> 00:02:01,660
+تستخدم بالإضافة لل blood film بتستخدم to help in
+
+26
+00:02:01,660 --> 00:02:05,820
+the differential diagnosis of anemia إذا تساعدني
+
+27
+00:02:05,820 --> 00:02:10,420
+لحتى أفرق ما بين أنواع الأنيميا و أشخص نوع
+
+28
+00:02:10,420 --> 00:02:15,980
+الأنيميا الصحيح أو الدقيق تمام؟The relationships
+
+29
+00:02:15,980 --> 00:02:20,700
+between hematocrit, homoglobin level and RBCs
+
+30
+00:02:20,700 --> 00:02:25,020
+count are converted to blood cell indices through
+
+31
+00:02:25,020 --> 00:02:29,320
+mathematical formulas تمام؟ احنا حكينا أنا بقيم
+
+32
+00:02:29,320 --> 00:02:33,360
+حجم الخليةمحتوها من الـ Homoglobin بالـ blood film
+
+33
+00:02:33,360 --> 00:02:36,820
+عن طريق الـ morphology الشكلها طبعا أنا بحفظ شكلها
+
+34
+00:02:36,820 --> 00:02:41,820
+خلية RBCs تلتينها Homoglobin تلتها Central Baller
+
+35
+00:02:41,820 --> 00:02:46,680
+و بيصير حجمها مقلوف لإلي طبعا بالنسبة لل Red blood
+
+36
+00:02:46,680 --> 00:02:50,000
+cell indices كيف أنا بقيم ال size و ال Homoglobin
+
+37
+00:02:50,000 --> 00:02:54,630
+content من خلالحسابات من خلال mathematical formula
+
+38
+00:02:54,630 --> 00:03:00,510
+صياغ رياضية تمام استخدم فيها أو تم استخدام فيها ال
+
+39
+00:03:00,510 --> 00:03:04,850
+hematocrit و ال hemoglobin level و ال RBCs إذا تم
+
+40
+00:03:04,850 --> 00:03:10,390
+توظيف ال hematocrit ال hemoglobin level و أيضا ال
+
+41
+00:03:10,390 --> 00:03:16,910
+RBCs count لحتىاللي بتم صياغة معادلات حسابية بتأشر
+
+42
+00:03:16,910 --> 00:03:21,930
+أو بتدل أو بتعتبر كمعاملات تعبر عن حجم الخلية
+
+43
+00:03:21,930 --> 00:03:27,690
+ومحتوها من ال homoglobin تمام؟ إذا لنرتب أفكارنا
+
+44
+00:03:27,690 --> 00:03:31,770
+red blood cell indices هي عبارة عن قياسات أو
+
+45
+00:03:31,770 --> 00:03:36,890
+مؤشرات أو معاملات تعبر عن حجم الخلية ومحتوها من ال
+
+46
+00:03:36,890 --> 00:03:40,050
+homoglobin بتفيدني لحتى أعمل differential
+
+47
+00:03:40,050 --> 00:03:45,840
+diagnosis للأنمياتمام؟ وكيف انا بحصل على هاي
+
+48
+00:03:45,840 --> 00:03:49,960
+القياسات او هاي المؤشرات تم توظيف ال hemoglobin و
+
+49
+00:03:49,960 --> 00:03:53,300
+ال level او ال hemoglobin concentration و ال
+
+50
+00:03:53,300 --> 00:03:58,440
+hematocrit و ال RBC count من خلال صيغ .. صيغ
+
+51
+00:03:58,440 --> 00:04:04,820
+رياضيةمن خلال معرفة العلاقة ما بين هاي ال .. ال ..
+
+52
+00:04:04,820 --> 00:04:08,340
+المكونات أو هاي الحسابات الهيموتوكرت وال
+
+53
+00:04:08,340 --> 00:04:13,080
+hemoglobin level و ال RBC count طم صياغة ال .. ال
+
+54
+00:04:13,080 --> 00:04:16,300
+indices هاي طب شو هي ال indices هاي؟ المؤشرات هاي
+
+55
+00:04:16,300 --> 00:04:20,320
+عبارة عن إيه؟ هي تلت مؤشرات ال mean corpuscular
+
+56
+00:04:20,320 --> 00:04:26,190
+volumeو ال mcv و ال mean corpuscular homoglobin و
+
+57
+00:04:26,190 --> 00:04:30,210
+ال mean corpuscular homoglobin concentration mcv
+
+58
+00:04:30,210 --> 00:04:36,630
+mch mchc طبعا طيب ال mean متوسط معناها corpuscular
+
+59
+00:04:36,630 --> 00:04:42,450
+يعني يعني بتعود على كلمة كرة لإن احنا بنحكي عن
+
+60
+00:04:42,450 --> 00:04:46,790
+خلايا الدم الحمراء كريات الدم الحمراء و كريات الدم
+
+61
+00:04:46,790 --> 00:04:50,470
+البيضاء طبعا فإذا corpuscular يعني جاية من .. من
+
+62
+00:04:50,470 --> 00:04:54,750
+.. من ..من شيء كراوي أي يعبّر عن الخلية، تمام؟
+
+63
+00:04:54,750 --> 00:04:58,510
+يعني هو متوسط، اذا mean carboscular volume هاي ..
+
+64
+00:04:58,510 --> 00:05:03,090
+هاي Syria معناها متوسط حجم الخلية، mean
+
+65
+00:05:03,090 --> 00:05:07,570
+carboscular homoglobin متوسط حجم ال .. او متوسط
+
+66
+00:05:07,570 --> 00:05:12,050
+سوري ال homoglobin content ل الخلية او الكريه
+
+67
+00:05:12,050 --> 00:05:15,130
+تمام؟ و ال mean carboscular homoglobin
+
+68
+00:05:15,130 --> 00:05:19,330
+concentration متوسط تركيز ال homoglobin في الكريه
+
+69
+00:05:19,330 --> 00:05:26,640
+او في الخليةتمام؟ اذا MCV, MCH, MCHC هدول هم ال
+
+70
+00:05:26,640 --> 00:05:31,060
+indices هدول هم المؤشرات و القياسات اللي لهم صياغ
+
+71
+00:05:31,060 --> 00:05:35,740
+و معادلات حسابية باستخدام ال hemotocrit و ال
+
+72
+00:05:35,740 --> 00:05:40,140
+homoglobin level و RBC count انا ممكن احسبهم تمام؟
+
+73
+00:05:40,140 --> 00:05:43,920
+و بيدللولي على حجم الخلية و على محتوها من ال
+
+74
+00:05:43,920 --> 00:05:49,210
+homoglobin و هذا بساعدني بتشخيص القنينة تمام؟نيجي
+
+75
+00:05:49,210 --> 00:05:54,450
+لال bare bones الغرض من هاي ال indices في البداية
+
+76
+00:05:54,450 --> 00:05:59,630
+نعرف إيش يعني anemia anemia include a variety of
+
+77
+00:05:59,630 --> 00:06:04,610
+conditions with the same outcome يعني ال .. ال
+
+78
+00:06:04,610 --> 00:06:11,330
+anemia ممكن يكون أسبابها عديدة ممكن يكون سببها إما
+
+79
+00:06:11,330 --> 00:06:18,130
+نقص في تصنيع ال homoglobin نقص في ال ironالخلل في
+
+80
+00:06:18,130 --> 00:06:23,810
+تصنيع ال homoglobin حتى كمان ممكن يكون shortage أو
+
+81
+00:06:23,810 --> 00:06:29,450
+حتى ال life span يعني قصر في عمر الخلية تمام ال
+
+82
+00:06:29,450 --> 00:06:35,170
+life span قليلة ل ال RBCs ممكن يكون
+
+83
+00:06:38,790 --> 00:06:45,690
+الخلية تفتقد عنصر ال folic acid أو ال vitamin B12
+
+84
+00:06:45,690 --> 00:06:49,550
+اللي بيلزمها لحتى تنقسم داخل البون مارو وتنضج
+
+85
+00:06:49,550 --> 00:06:54,410
+وتخرج إلى ال circulation تمام؟ إذا ممكن إن ال RBC
+
+86
+00:06:54,410 --> 00:06:58,550
+بتتصنع طبيعي عددها طبيعي و ال homoglobin تباعها
+
+87
+00:06:58,550 --> 00:07:03,560
+طبيعي بس لما بتطلع علىال .. ال circulation بتتكسر
+
+88
+00:07:03,560 --> 00:07:10,380
+بسرعة، إذا عندي أسباب عديدة لإن .. نحكي عنها إنها
+
+89
+00:07:10,380 --> 00:07:14,220
+بتسببلي أنميا، تمام؟ إذا إن هي عبارة عن variety of
+
+90
+00:07:14,220 --> 00:07:19,340
+conditions، في ظروف عديدة ممكن تسببلي أنميا ولكن
+
+91
+00:07:19,340 --> 00:07:24,840
+المحصلة كلها يتم وصفها بإنه ال blood can't carry
+
+92
+00:07:24,840 --> 00:07:29,350
+as much oxygen as it shouldال .. ال .. ال blood
+
+93
+00:07:29,350 --> 00:07:33,890
+كمية الدم و كمية ال RBCs اللي موجودة لا تحمل
+
+94
+00:07:33,890 --> 00:07:39,130
+أكسوجين كافي إلى ال tissues تمام كما ينبغي as it
+
+95
+00:07:39,130 --> 00:07:44,390
+should فبالتالي الشخص بيبدأ يعاني من hypoxia إيش
+
+96
+00:07:44,390 --> 00:07:49,040
+يعني hypoxia؟ يعني نقص أكسوجين في الجسمالسبب إنه
+
+97
+00:07:49,040 --> 00:07:52,840
+إما ال RBCs عمرها قصير أو بتتكسر بسرعة أو بيتم
+
+98
+00:07:52,840 --> 00:07:58,320
+تصنيعها بشكل مش كافي ال homoglobin بيتصنع بشكل
+
+99
+00:07:58,320 --> 00:08:02,420
+قليل أو يعني بتركيز قليل أو حتى كمان بيتصنعوا في
+
+100
+00:08:02,420 --> 00:08:06,640
+خطأ في تصنيعه مافيش iron كفاية لل homoglobin لإنه
+
+101
+00:08:06,640 --> 00:08:09,620
+حكينا ال iron أساسي لتركيب ال homoglobin اللي هو
+
+102
+00:08:09,620 --> 00:08:13,600
+أصلا أساسي لحمل ال oxygenتمام؟ الخلايا و هي عم
+
+103
+00:08:13,600 --> 00:08:18,120
+تنقسم صار فيها خلال معين هذه كلها conditions تمام؟
+
+104
+00:08:18,120 --> 00:08:22,680
+المحصلة hypoxia اذا و بيصير ان ال blood can't
+
+105
+00:08:22,680 --> 00:08:28,000
+carry oxygen as much as it should تمام؟أما ال
+
+106
+00:08:28,000 --> 00:08:31,660
+healthy person الشخص الطبيعي ال normal has
+
+107
+00:08:31,660 --> 00:08:35,260
+adequate number of correctly sized red blood cell
+
+108
+00:08:35,260 --> 00:08:38,700
+that contain enough hemoglobin to carry sufficient
+
+109
+00:08:38,700 --> 00:08:44,150
+oxygen at all the body's tissuesتمام الشخص الطبيعي
+
+110
+00:08:44,150 --> 00:08:48,830
+عنده adequate number، عدد طبيعي وكافي من ال RBCs،
+
+111
+00:08:48,830 --> 00:08:52,610
+اللي حجمها طبيعي و اللي محتوها من ال homoglobin
+
+112
+00:08:52,610 --> 00:08:56,850
+أيضا كافي فبالتالي هي بتحمل oxygen sufficient،
+
+113
+00:08:56,850 --> 00:09:02,490
+كافي لل all body tissues، لكل أنسجة الجسمتمام an
+
+114
+00:09:02,490 --> 00:09:06,970
+animic person has red blood cells that are either
+
+115
+00:09:06,970 --> 00:09:11,190
+too small or too few in number الشخص اللي .. اللي
+
+116
+00:09:11,190 --> 00:09:15,270
+.. اللي بنحكي عنه animic عنده أنيميا ال .. ال red
+
+117
+00:09:15,270 --> 00:09:18,510
+blood cell تبعته ممكن تكون صغير، حجمها صغير كتير
+
+118
+00:09:18,510 --> 00:09:21,630
+فبالتالي محتوىها من ال homoglobin هيكون قليل أو
+
+119
+00:09:21,630 --> 00:09:28,250
+ممكن يكون عددها قليلطبعا إما نتيجة ضعف الإنتاج أو
+
+120
+00:09:28,250 --> 00:09:32,050
+حتى ممكن لإنها ال life span حكينا فيها shortage
+
+121
+00:09:32,050 --> 00:09:35,550
+قصيرة المتوسط عمر الخلية تطلع ع ال circulation
+
+122
+00:09:35,550 --> 00:09:41,860
+تتكسر بسرعة طبعا بيصيرلها licenseف whatever السبب
+
+123
+00:09:41,860 --> 00:09:46,980
+في النهاية هذا كله بينوصف ك anemia ال anemia ممكن
+
+124
+00:09:46,980 --> 00:09:53,540
+تكون كمرض منفصل بحد ذاته او ممكن تكون عرض لمرض اخر
+
+125
+00:09:53,540 --> 00:09:57,710
+تمام؟ممكن تكون احد اعراض مرض معين او ممكن تكون مرض
+
+126
+00:09:57,710 --> 00:10:02,570
+بحد ذاتها فبالتالي اذا لما بيكون عندى الخلايا اما
+
+127
+00:10:02,570 --> 00:10:07,610
+صغيرة او قليلة او مابتحملش اكسوجين بشكل كافي ممكن
+
+128
+00:10:07,610 --> 00:10:13,190
+هذا يشكل جهد على ال heart و ال lung must work
+
+129
+00:10:13,190 --> 00:10:17,010
+harder تمام؟ اذا فى جهد عليهم لحتى يعوضوا ال
+
+130
+00:10:17,010 --> 00:10:23,750
+oxygen اللى بيتم فقدانه او افتقاده في الجسمanemia
+
+131
+00:10:23,750 --> 00:10:27,370
+is caused by many different diseases or disorders
+
+132
+00:10:27,370 --> 00:10:30,690
+حكينا هذه المعلومة حكينا ان الانيميا ممكن تكون ك
+
+133
+00:10:30,690 --> 00:10:37,030
+disease منفصل بحد ذاته او ممكن تكون يعني عرض من
+
+134
+00:10:37,030 --> 00:10:40,770
+اعراض disease او disorder اخر the first step in
+
+135
+00:10:40,770 --> 00:10:44,230
+finding the cause is determine what type of anemia
+
+136
+00:10:44,230 --> 00:10:50,210
+طبعا حتى انا اول خطوة بشخص فيها الانيميا بدي اعرف
+
+137
+00:10:50,550 --> 00:10:55,430
+وصف هاي ال anemia تمام؟ يعني هى anemia تسببت في
+
+138
+00:10:55,430 --> 00:11:02,490
+قلة فى ال RBC فقط او anemia تسببت بصغر فى حجم ال
+
+139
+00:11:02,490 --> 00:11:06,990
+RBC او قلة فى تركيز ال homoglobin هدى كلها مين
+
+140
+00:11:06,990 --> 00:11:10,110
+اللى هيدلللي عليها؟ مين اللى هيصفلي هاى النوع ال
+
+141
+00:11:10,110 --> 00:11:14,550
+anemia ال red blood cell indices هى اللى هتساعدني
+
+142
+00:11:14,550 --> 00:11:20,910
+to classify the anemia لحتى أصنف ال anemiaمع خلايا
+
+143
+00:11:20,910 --> 00:11:24,970
+صغيرة الحجم أنينيا مع خلايا تركيز ال homoglobin
+
+144
+00:11:24,970 --> 00:11:29,110
+قليل فيها أنينيا تركيز ال homoglobin طبيعي و حجمها
+
+145
+00:11:29,110 --> 00:11:33,890
+طبيعي بس عم تتكسر بسرعة فبالتالي في عندى فقط few
+
+146
+00:11:33,890 --> 00:11:39,700
+number فيها ولا شو بالضبطتمام؟ فإذا الإجابة راح
+
+147
+00:11:39,700 --> 00:11:44,940
+تكون نوع الأنيميا هاي أو الوصف لهذه الأنيميا عند
+
+148
+00:11:44,940 --> 00:11:49,460
+ال red blood cell indices مؤشرات خلايا الدم
+
+149
+00:11:49,460 --> 00:11:54,950
+الحمراءنبدأ مع أول معامل أو مؤشر اللي هو ال mean
+
+150
+00:11:54,950 --> 00:11:59,950
+carboscular volume حكينا متوسط حجم الكورية أو
+
+151
+00:11:59,950 --> 00:12:04,650
+الخلية تمام اللي هو ال MCV تعريفه is the average
+
+152
+00:12:04,650 --> 00:12:10,870
+volume of herbicides طبعا تركيزه بيتم قياس التركيز
+
+153
+00:12:10,870 --> 00:12:15,650
+أو وحدة القياس هي الفيمتو لتر تمام الفيمتو لتر و
+
+154
+00:12:15,650 --> 00:12:19,910
+الفيمتو لتر هو عبارة عن عشرة أسالب خمستاش من ال
+
+155
+00:12:19,910 --> 00:12:26,460
+letterطبعا طيب ال MCV هو عبارة عن إيش؟ طيب خلينا
+
+156
+00:12:26,460 --> 00:12:30,820
+بس نستنتج القوانين هذه ال mathematical formulas
+
+157
+00:12:30,820 --> 00:12:36,400
+القوانين الرياضية هاي بشكل منطقي جدااتعرفنا على ال
+
+158
+00:12:36,400 --> 00:12:41,340
+hematocrit وحكينا هو الحجم أو الحيز اللي بيشغل ..
+
+159
+00:12:41,340 --> 00:12:46,540
+تشغل ال RBCs كلها بالنسبة للعينة تمام؟ وإحنا لما
+
+160
+00:12:46,540 --> 00:12:50,480
+كنا بنحسب ال hematocrit كنا بنفترض إن العينة كلها
+
+161
+00:12:50,480 --> 00:12:56,300
+100% و بنشوف كام نسبة بتشغل ال RBCs من هذه العينة
+
+162
+00:12:56,300 --> 00:12:59,820
+ككلتمام؟ طيب يعني إذا ال hematocrite هو حجم ال
+
+163
+00:12:59,820 --> 00:13:04,160
+RBCs ككل كل ال RBCs هيك بنصفطها على بعض و بنشوف
+
+164
+00:13:04,160 --> 00:13:09,360
+حجمها تمام؟ بالمية طيب ال RBCs count عدد خلايا
+
+165
+00:13:09,360 --> 00:13:15,220
+الدم الحمراء طيب لو أنا .. طب لو قسمت الحجم اللي
+
+166
+00:13:15,220 --> 00:13:21,520
+بتشغله ال RBCs كلها على عددها ككل مش بالنهاية بحصل
+
+167
+00:13:21,520 --> 00:13:26,700
+على الحجم اللي بتحتله ال RBCs الواحدةفبالنهاية انا
+
+168
+00:13:26,700 --> 00:13:32,380
+بحصل على ال average volume of RBCs يعني متوسط لحجم
+
+169
+00:13:32,380 --> 00:13:36,860
+خلية واحدة من ال RBCs تمام؟ اذا بقسم الحجم اللي
+
+170
+00:13:36,860 --> 00:13:41,960
+بتحتله ال RBCs كلها على عدد هاي ال RBCs و بالنهاية
+
+171
+00:13:41,960 --> 00:13:46,320
+بحصل على متوسط حجم الخلية طبعا بدرب الاشي هذا
+
+172
+00:13:46,320 --> 00:13:53,530
+بعشرة عشان في النهاية انا احصل على يعني ال ..الرقم
+
+173
+00:13:53,530 --> 00:13:58,110
+يكون منطقي فقط طيب إذا ال .. ال .. ال hemotocrit
+
+174
+00:13:58,110 --> 00:14:00,830
+هاي هنا مثال بيعطيني hemotocrit خمسة و أربعين في
+
+175
+00:14:00,830 --> 00:14:07,670
+المية و ال RBC's خمسة مليون per liter طيب ال MCV
+
+176
+00:14:07,670 --> 00:14:11,910
+هي عبارة عن إيش؟ هي عبارة عن الخمسة و أربعين على
+
+177
+00:14:11,910 --> 00:14:15,230
+مين؟ على ال millions فقط يعني على فقط العدد هذا
+
+178
+00:14:15,230 --> 00:14:18,750
+أنا مابستخدم العشرة أقصد اتناش في الحسابات طيب هو
+
+179
+00:14:18,750 --> 00:14:23,670
+صح خمسة مليونسل بير مايكرو ليتر تمام؟ هي أقصى
+
+180
+00:14:23,670 --> 00:14:27,570
+اتناش عشان bear microliter .. bear liter هنا sorry
+
+181
+00:14:27,570 --> 00:14:31,410
+هي عشرة أقصى اتناش عشانها bear liter لكن لو كانت
+
+182
+00:14:31,410 --> 00:14:36,560
+مثلا bear microliter هتكون عشرة أقصى ستةطيب ف ..
+
+183
+00:14:36,560 --> 00:14:39,880
+في ال .. ما .. سواء كانت per liter أو per
+
+184
+00:14:39,880 --> 00:14:43,720
+microliter الرقم اللي هنا اللي هو عشر أقص أنا
+
+185
+00:14:43,720 --> 00:14:47,940
+مابدياه، تمام؟ أنا فقط بدي .. كام مليون؟ هم خمسة
+
+186
+00:14:47,940 --> 00:14:51,500
+مليون، خلاص أنا باخد الخمسة، تمام؟ اذا هنا ال
+
+187
+00:14:51,500 --> 00:14:55,740
+RBC's count أنا فقط بدي عدد المليونات، كام مليون؟
+
+188
+00:14:55,740 --> 00:15:01,920
+خمسة مليون، خلاص خمسة فقططمام؟ ف45 على 5 فبيطلع 9
+
+189
+00:15:01,920 --> 00:15:06,140
+ضرب 10 اذا 90 fem to liter هو الحجم طيب ال normal
+
+190
+00:15:06,140 --> 00:15:11,020
+range ل MCV بيقع ما بين 80 إلى 100 اذا ال MCV
+
+191
+00:15:11,020 --> 00:15:16,710
+normal ما بين 80 إلى 100طيب هذه الخلية الطبيعية
+
+192
+00:15:16,710 --> 00:15:21,750
+اللي يعني حجمها بيتراوح ما بين 80 femtoliter إلى
+
+193
+00:15:21,750 --> 00:15:27,390
+100 femtoliter طبعا و ممكن يكون 85 ممكن يكون 90
+
+194
+00:15:27,390 --> 00:15:32,250
+اذا it's as a range طبعا طيب لو .. لو افترض انه
+
+195
+00:15:32,250 --> 00:15:40,640
+طلع عندي الخلية حجمهاأقل من 80 تمام؟ إذا كان أقل
+
+196
+00:15:40,640 --> 00:15:46,540
+من 80 معناها خلية صغيرة إذا هذا ال normal range و
+
+197
+00:15:46,540 --> 00:15:49,880
+كانت الخلية أقل من 80 يعني أقل من ال lower limit
+
+198
+00:15:49,880 --> 00:15:54,260
+لل range معناها الخلية صغيرة في الحجم طيب إيش
+
+199
+00:15:54,260 --> 00:15:58,220
+المصطلح اللي بيصف إن الخلية تكون صغيرة ال smaller
+
+200
+00:15:58,220 --> 00:16:02,420
+cell إن هي تكون microcytic إذا لما أحكي عن خلاء
+
+201
+00:16:02,420 --> 00:16:07,290
+خلية صغيرة في الحجمبحكي عنها إن هي micro يعني
+
+202
+00:16:07,290 --> 00:16:11,210
+صغيرة micro طبعا citic .. citic جاية من كلمة site
+
+203
+00:16:11,210 --> 00:16:17,450
+أو خلية طبعا micro citic خلية صغيرة طيب لو كان ال
+
+204
+00:16:17,450 --> 00:16:22,910
+MCV أكتر من مية more than one hundred فهمته later
+
+205
+00:16:23,190 --> 00:16:28,010
+طيب هتنحكي عنها larger cell اللي هي macrocytic
+
+206
+00:16:28,010 --> 00:16:32,590
+طبعا إذا micro يعني صغير، macro كبير، cytic خلية
+
+207
+00:16:32,590 --> 00:16:38,130
+microcytic خلية صغيرة و macrocytic خلية كبيرة و
+
+208
+00:16:38,130 --> 00:16:41,610
+هلأ استنا بنعرف إيش يعني خلية صغيرة و إيش يعني
+
+209
+00:16:41,610 --> 00:16:47,410
+خلية كبيرة microcytic يعني ال MCV تبعها أقل من 80و
+
+210
+00:16:47,410 --> 00:16:53,670
+macrocytic ال MCV خلية ال .. ال MCV أكثر من مائة
+
+211
+00:16:53,670 --> 00:16:59,330
+تمام؟ ال microcytic خلية ال MCV أقل من تمانين و ال
+
+212
+00:16:59,330 --> 00:17:07,010
+macrocytic خلية ال MCV أكثر من مائة طيب إذا هنا بس
+
+213
+00:17:07,010 --> 00:17:14,080
+املخصلي إنه normocyticعفوان ال normocytic هي عبارة
+
+214
+00:17:14,080 --> 00:17:18,600
+عن يعني أو وصفلي normocytic anemia إيش يعني؟ يعني
+
+215
+00:17:18,600 --> 00:17:24,620
+خلية ال .. ال RBCs فيها normal size normocytic
+
+216
+00:17:24,620 --> 00:17:31,160
+anemia يعني خلية ال .. ال MCV normal عندها، تمام؟
+
+217
+00:17:31,160 --> 00:17:37,030
+معناهاالخلل اللي سبب لي أنيميا مش في حجم ال .. ال
+
+218
+00:17:37,030 --> 00:17:41,290
+RBCs طبعا احنا هنا حكينا ان ال RBCs .. ان الانيميا
+
+219
+00:17:41,290 --> 00:17:44,750
+ممكن تنتج عن ال small او ال RBCs تكون if you ال
+
+220
+00:17:44,750 --> 00:17:48,490
+number طبعا و ممكن تكون ال .. ال low concentration
+
+221
+00:17:48,490 --> 00:17:52,930
+of homoglobin طبعا فإذا لما نحكي عن normostatic
+
+222
+00:17:52,930 --> 00:17:58,510
+أنيميا معناها هي عبارة عن أنيميا الخلية حجمها
+
+223
+00:17:58,510 --> 00:18:04,520
+normalإذا الخلل إما هيكون في ال life span، في عمر
+
+224
+00:18:04,520 --> 00:18:07,400
+الخلية أو ممكن يكون في ال homoglobin content،
+
+225
+00:18:07,400 --> 00:18:15,620
+تمام؟طيب microcytic anemia إذا هي أنيمية نتجت عن
+
+226
+00:18:15,620 --> 00:18:19,640
+إنه الخلايا small cells يعني في عندها decreased
+
+227
+00:18:19,640 --> 00:18:24,820
+MCV تمام أما ال macrocytic anemia فهي عبارة عن
+
+228
+00:18:24,820 --> 00:18:29,980
+أنيمية نتجت عن خلايا large cell كبيرة يعني
+
+229
+00:18:29,980 --> 00:18:35,520
+increased MCV طيب نتسأل هل الخلية ال RBCs لما تكون
+
+230
+00:18:35,520 --> 00:18:39,690
+كبيرة ممكن ينتج أنيمية؟بتكون كبيرة وفاضية على
+
+231
+00:18:39,690 --> 00:18:42,670
+الفاضي يعني حجم تمام فبالتالي .. يعني مافيش فيها
+
+232
+00:18:42,670 --> 00:18:47,150
+homoglobin كافي فبالتالي بتنتجلي أنيميا منها تمام
+
+233
+00:18:47,150 --> 00:18:50,590
+طب إيش أمثلة على ال microcytic anemia ال
+
+234
+00:18:50,590 --> 00:18:54,830
+microcytic anemia هي مثال عليها ال iron deficiency
+
+235
+00:18:54,830 --> 00:18:59,290
+أنيميا و السلسيميا تمام ال iron deficiency أنيميا
+
+236
+00:18:59,290 --> 00:19:03,490
+اللي هي أنيميا نقص الحديد و السلسيميا اللي هي ال
+
+237
+00:19:03,490 --> 00:19:09,830
+..Anemia البحر الأبيض المتوسط هدول التنتين بنسميهم
+
+238
+00:19:09,830 --> 00:19:13,890
+microcytic الخلايا بتكون فيها صغيرة و ال
+
+239
+00:19:13,890 --> 00:19:17,330
+homoglobin content فيها قليل تمام ال homoglobin
+
+240
+00:19:17,330 --> 00:19:22,960
+فيها قليل ليش؟ لإن هنا ماعنديش iron كفايةفال .. ال
+
+241
+00:19:22,960 --> 00:19:26,400
+homoglobin اللي بيتصنع ما بيكونش ما .. إله iron
+
+242
+00:19:26,400 --> 00:19:32,140
+فبالتالي بيكون فيه نقص في ال .. ال iron فبت .. في
+
+243
+00:19:32,140 --> 00:19:36,760
+ال homoglobin كمان و نقص في ال oxygen delivery
+
+244
+00:19:36,760 --> 00:19:42,060
+الثلاثيمية هي طفرة في جينات ال .. ال homoglobin
+
+245
+00:19:42,060 --> 00:19:46,880
+فبيتم تصنيع .. بيصير فيه decrease production من ال
+
+246
+00:19:46,880 --> 00:19:52,610
+homoglobinأو ممكن يكون الطفرة أعطتني هموجلوبين
+
+247
+00:19:52,610 --> 00:19:55,890
+الشكل تبعه و التركيب تبعه abnormal فبالتالي
+
+248
+00:19:55,890 --> 00:20:00,570
+الوظيفة تبعته هتكون abnormal ما بتحمل يعني أكسجين
+
+249
+00:20:00,570 --> 00:20:04,930
+طيب ال macrocytic anemia أو ال macrocytic cells
+
+250
+00:20:04,930 --> 00:20:09,570
+طيب أنيميا نتجت عن خلايا كبيرة في الحجم هذه
+
+251
+00:20:09,570 --> 00:20:14,030
+بنشوفها في liver disease وفي الhypothyroidism تمام
+
+252
+00:20:14,030 --> 00:20:19,650
+يعنيخلايا الثيرويد خلايا الغداددرقية هرموناتها
+
+253
+00:20:19,650 --> 00:20:23,390
+قليلة بتنتج هرمونات قليلة هي معناه hypo hypo قليل
+
+254
+00:20:23,390 --> 00:20:28,870
+thyroidism يعني هرمونات الغداددرقية تمام ال liver
+
+255
+00:20:28,870 --> 00:20:33,590
+disease مشكلة في الكبد ممكن ينتج عنها ان الخلايا
+
+256
+00:20:33,590 --> 00:20:39,410
+بتكون macrocytic كبيرة طيب ممكن لما يكون في عندي
+
+257
+00:20:39,410 --> 00:20:43,890
+asynchronous in RBCs maturationيعني مافيش تناسق في
+
+258
+00:20:43,890 --> 00:20:48,470
+نضوج ال RBCs تمام؟ و هذا بنسميه megaloplastic
+
+259
+00:20:48,470 --> 00:20:52,230
+anemia و هذه ال megaloplastic anemia يعني خلايا
+
+260
+00:20:52,230 --> 00:20:56,230
+كبيرة تمام؟ و لكن في عندي anemia يعني مافيش
+
+261
+00:20:56,230 --> 00:21:01,330
+همجلبين او يعني انه مافيش RBCs كافية تمام؟
+
+262
+00:21:01,330 --> 00:21:05,190
+فبالتالي هذه بتنتج عن ال folate عن ال folic acid و
+
+263
+00:21:05,190 --> 00:21:08,930
+ال vitamin B12 deficienciesطبعا خلينا بس نحكي
+
+264
+00:21:08,930 --> 00:21:13,370
+حدوطة صغيرة عشان نعرف إيش يعنيه إنه مافيش تناسق
+
+265
+00:21:13,370 --> 00:21:19,730
+هلأ ال RBCs أثناء نضوجها في ال bone marrow بتمر
+
+266
+00:21:19,730 --> 00:21:23,450
+بعدة مراحل بتكون عندي خلية أم إحنا متفقين إنه في
+
+267
+00:21:23,450 --> 00:21:27,890
+عندي خلية أم لكل نوع من أنواع ال blood cells فعيني
+
+268
+00:21:27,890 --> 00:21:33,260
+في عندي خلية أم ل ال RBCsهذه الخلية الأم مجرد ما
+
+269
+00:21:33,260 --> 00:21:39,760
+الجسم يحتاج RBCs بيرسللها رسالة فبتتنشط و بتبدأ
+
+270
+00:21:39,760 --> 00:21:44,100
+تنقسم و تندوج لحتى تعطيني RBCs و ال RBCs هادي
+
+271
+00:21:44,100 --> 00:21:47,120
+بتتخرج و بتطلع من ال .. من ال boomer و بتطلع ع ال
+
+272
+00:21:47,120 --> 00:21:53,060
+circulation تمام؟ طيب .. هلأ أثناء ندوج الخلايا
+
+273
+00:21:53,060 --> 00:21:58,630
+هاي ال ..اللي خلّيني نحكي عنها ال progenitors اللي
+
+274
+00:21:58,630 --> 00:22:04,370
+هي يعني الخلايا السلالة الخلايا الأصل لل RBCs
+
+275
+00:22:04,370 --> 00:22:11,490
+تمام؟ أثناء نضوجها بتكون إلها نواة يعني لما بتصل
+
+276
+00:22:11,490 --> 00:22:15,150
+لآخر مراحل في النضوج بتفقد النواة هذه عشان هيك ال
+
+277
+00:22:15,150 --> 00:22:21,310
+RBCs بتطلع على ال .. ال circulation تمام؟ بتطلع
+
+278
+00:22:21,310 --> 00:22:30,420
+فاقدة نواتها مافيش فيها نواة تمام؟و ال .. أثناء
+
+279
+00:22:30,420 --> 00:22:36,420
+أيضا ندوجها الخلية بيصغر حجمها، تمام؟ بتكون كبيرة،
+
+280
+00:22:36,420 --> 00:22:39,560
+الخلايا ال mother، الأم في المراحل الأولى من
+
+281
+00:22:39,560 --> 00:22:43,540
+الندوج بتكون كبيرة، بتبدأ تصغر، تصغر، تصغر، بعدين
+
+282
+00:22:43,540 --> 00:22:48,740
+بتفقد النواة، بعدين بتطلع على ال circulationإذن في
+
+283
+00:22:48,740 --> 00:22:53,060
+عندي أحداث بت .. بتصير أثناء ال maturation اللي هي
+
+284
+00:22:53,060 --> 00:22:56,820
+أول حاجة صغر في الحجم تدريجي لحد ما تصل لعندي ال
+
+285
+00:22:56,820 --> 00:23:00,860
+MCV ال normal اللي هي من 80 ل 100 و بعدين بتطلع ال
+
+286
+00:23:00,860 --> 00:23:09,270
+circulation و أيضا ..بيكون في فقدان للنواة و أيضا
+
+287
+00:23:09,270 --> 00:23:15,470
+بيكون في عندى ال homoglobin .. تصنيع لل
+
+288
+00:23:15,470 --> 00:23:19,170
+homoglobin، تمام؟ homoglobin synthesis فال
+
+289
+00:23:19,170 --> 00:23:23,210
+homoglobin synthesis هذا ما بيضل شغال على طول، لأ
+
+290
+00:23:23,980 --> 00:23:27,480
+الخلية لما بتطلع من ال .. ال .. ال bone marrow
+
+291
+00:23:27,480 --> 00:23:32,120
+لازم تكون وصلة لعين تركيز معين من ال homoglobin E
+
+292
+00:23:32,120 --> 00:23:35,760
+كف .. كفيل انه يخرجها من ال bone marrow و تروح ع
+
+293
+00:23:35,760 --> 00:23:42,560
+ال circulation، طبعا، احنا هناكطلاب مثلا بتدرسوا،
+
+294
+00:23:42,560 --> 00:23:47,560
+بتنضجوا، بتتعلموا، بتكتسبوا خبرات، تمام؟ بتجتازوا
+
+295
+00:23:47,560 --> 00:23:52,480
+امتحانات و لما نصل لعند مرحلة معينة من العلم عندكم
+
+296
+00:23:52,480 --> 00:23:56,660
+كافية أننا نخرجكم، تتخرجوا، تمام؟ نفس الاشي ال
+
+297
+00:23:56,660 --> 00:24:05,460
+RBCsبتنضج، بتنقسم و بتفقد النواء تبعتها، بتصنع
+
+298
+00:24:05,460 --> 00:24:09,220
+هموجلوبين، الهموجلوبين هذا بيصل لمستوى كافي إنها
+
+299
+00:24:09,220 --> 00:24:14,040
+تطلع من ال bone marrow فتطلع، تمام؟ طيبلو كان في
+
+300
+00:24:14,040 --> 00:24:18,560
+عندي نقص في ال .. ال iron طبعا، لو كان في عندي نقص
+
+301
+00:24:18,560 --> 00:24:21,580
+في ال iron أو حتى نقص في تصنيع ال homoglobin ذات
+
+302
+00:24:21,580 --> 00:24:26,880
+نفسه، الخلية بتضلها تنقسم، تنقسم، تنقسم ل .. لحد
+
+303
+00:24:26,880 --> 00:24:30,240
+.. يعني بدها تصل لمرحلة إنه يكون ال homoglobin
+
+304
+00:24:30,240 --> 00:24:35,020
+اللي موجود فيها كافي إنها تتخرج، طبعا؟ كافي إنها
+
+305
+00:24:35,020 --> 00:24:39,500
+تطلع من ال bone marrowطبعا فبتضطر انها تبضل تنقسم
+
+306
+00:24:39,500 --> 00:24:45,220
+تنقسم لحد ما تصل لحجم كتير صغير عليها انه يكون ال
+
+307
+00:24:45,220 --> 00:24:49,220
+homoglobin اللي فيها كافي انها تطلع عشان يطلعها ال
+
+308
+00:24:49,220 --> 00:24:55,610
+bone marrow طبعا فعشان هيك في حالة نقصان ال ironو
+
+309
+00:24:55,610 --> 00:24:59,150
+في حال اتخلل في تصنيع ال homoglobin او نقصان في
+
+310
+00:24:59,150 --> 00:25:02,630
+تصنيع ال homoglobin تمام مافيش عندي homoglobin
+
+311
+00:25:02,630 --> 00:25:05,470
+كافي في ال bone marrow .. في ال .. ال RBCs في ال
+
+312
+00:25:05,470 --> 00:25:10,090
+bone marrow تمام فبالتالي ال RBCs اش اللي عمل
+
+313
+00:25:10,090 --> 00:25:15,330
+امامها ان هتظلها تنقسمتمام؟ انقسامات وانقسامات
+
+314
+00:25:15,330 --> 00:25:19,670
+وانقسامات لحد ما تصل لحجم كتير صغير ما هي كل ما
+
+315
+00:25:19,670 --> 00:25:24,250
+بتنقسم كل ما بصغر حجمها تمام؟ لحد ما تصل لحجم كتير
+
+316
+00:25:24,250 --> 00:25:28,350
+صغير و يكون شوية ال homoglobin اللي فيها كافية
+
+317
+00:25:28,350 --> 00:25:32,170
+عليها بالنسبة لحجمها طبعا كافية انها تتخرج و تطلع
+
+318
+00:25:32,170 --> 00:25:36,530
+من ال bone marrow تمام؟ فعشان هيك ال iron
+
+319
+00:25:36,530 --> 00:25:41,880
+deficiency و الthalassemiaبينتج عنها microcytic
+
+320
+00:25:41,880 --> 00:25:47,320
+cell خلية ال MCV تبعها قليل عرفنا ليش بسبب
+
+321
+00:25:47,320 --> 00:25:52,680
+الانقسامات الكثيرة لحتى تصل لل target تبعها اللي
+
+322
+00:25:52,680 --> 00:25:55,880
+هو ال homoglobin content اللي لازم تكون على الأقل
+
+323
+00:25:55,880 --> 00:26:02,400
+محتوية بالنسبة لحجمها طيبفي حالة الفولك أسد
+
+324
+00:26:02,400 --> 00:26:07,480
+ديفشانسي و ال vitamin B12 ديفشانسي إيش بيصير؟ طبعا
+
+325
+00:26:07,480 --> 00:26:13,600
+هدول يا بنات بيلزموا لانقسام النواء تمام؟ بيلزموا
+
+326
+00:26:13,600 --> 00:26:18,360
+الخلية أثناء انقسام النواء طبعتها طيب هدول تم
+
+327
+00:26:18,360 --> 00:26:23,380
+نقسانهم إذا النواء مش راح تنقسم إذا الخلية راح
+
+328
+00:26:23,380 --> 00:26:29,250
+تظلها كبيرةو احنا حاكينا كل ما تنقسم كل ما زغرت في
+
+329
+00:26:29,250 --> 00:26:36,130
+الحجم طيب في عندي عوامل أصلا يعني متطلبات و مهمة
+
+330
+00:26:36,130 --> 00:26:39,690
+جدا لانقسام النواة و في عندي deficiencies فيهم، في
+
+331
+00:26:39,690 --> 00:26:44,810
+نقصان فيهم، تمام؟طيب فبالتالي النواء مش راح تنقسم
+
+332
+00:26:44,810 --> 00:26:49,870
+فالخلية ممكن تفقد .. يعني راح تبقى تصنع homoglobin
+
+333
+00:26:49,870 --> 00:26:54,250
+و تصل ال target بس لسه حجمها كبير فبتفقد النواء
+
+334
+00:26:54,250 --> 00:27:00,450
+early تمام؟ بتم التخلص من نواتها و هي لسه حجمها
+
+335
+00:27:00,450 --> 00:27:06,600
+كبير تمام؟فهذا بنسميه asynchronous عدم تناسق ما
+
+336
+00:27:06,600 --> 00:27:10,420
+بين انقسام النواء ونضوج ال .. او ال .. ال
+
+337
+00:27:10,420 --> 00:27:13,180
+cytoplasm يعني انه هو يعني انه يكون فيه محتوى
+
+338
+00:27:13,180 --> 00:27:18,780
+هموجلوبين كافي تمام؟ فبينتج عنه اما microcytic او
+
+339
+00:27:18,780 --> 00:27:23,000
+macrocytic cells تمام؟ هذا ال asynchronous بينتج
+
+340
+00:27:23,000 --> 00:27:29,800
+عنه microcytic او macrocytic اذا تأخرتصنيع ال
+
+341
+00:27:29,800 --> 00:27:35,380
+homoglobin الكافي لتخريج ال RBCs من ال bone marrow
+
+342
+00:27:35,380 --> 00:27:41,140
+تستمر الخلية في الانقسام لحد ما تصل ل microcytic
+
+343
+00:27:41,140 --> 00:27:48,380
+تمام؟ وإذا تأخر نضوج النواء وانقسام النواءتستمر
+
+344
+00:27:48,380 --> 00:27:54,280
+الخلية بتصنيع ال homoglobin ولكن بعد هي بتضطر ..
+
+345
+00:27:54,280 --> 00:27:57,280
+بتصلح .. لا خلاص بتصلح ال target اللي كافيل بإنه
+
+346
+00:27:57,280 --> 00:28:00,920
+يخرجها من ال bone marrow فبتضطر إنها تتخلص من
+
+347
+00:28:00,920 --> 00:28:04,480
+النواء و هي لساتها حجمها كبير لإنها ما انقسمت
+
+348
+00:28:04,480 --> 00:28:09,020
+كفاية لإن العوامل اللي بتلزمها لانقسم نواتها أصلا
+
+349
+00:28:09,020 --> 00:28:13,620
+فيهم deficiency فالخلية بتطلع ليه macrocytic تمام؟
+
+350
+00:28:13,620 --> 00:28:19,980
+بتطلع macrocytic كبيرةطيب ال mean corpuscular
+
+351
+00:28:19,980 --> 00:28:25,880
+homoglobin متوسط ال homoglobin في الكورية طبعا؟ طب
+
+352
+00:28:25,880 --> 00:28:29,940
+متوسط ال homoglobin ايش؟ وزن ال homoglobin طبعا؟
+
+353
+00:28:29,940 --> 00:28:34,480
+the average weight of homoglobin in an RBCs طيب
+
+354
+00:28:34,480 --> 00:28:39,320
+وحدة القياس ال picogram طبعا؟ ال picogram و ال
+
+355
+00:28:39,320 --> 00:28:43,620
+picogram هو عبارة عن عشرة أسالب اتناش ملي جرام طيب
+
+356
+00:28:44,640 --> 00:28:50,320
+خلّينا نشوف طيب كتع .. يعني كتحليل منطقي لقى
+
+357
+00:28:50,320 --> 00:28:58,700
+القانون نشوف إيش يعني MCH ال MCH إحنا حكينا متوسط
+
+358
+00:28:58,700 --> 00:29:05,200
+وزن ال homoglobin في ال .. ال RBCs الواحدة تمام؟
+
+359
+00:29:05,200 --> 00:29:11,280
+طيب إحنا لما كنا نقيس ال homoglobin .. sorryلما
+
+360
+00:29:11,280 --> 00:29:14,720
+كنا نقيس ال homoglobin ايش كنا نحكي؟ كنا نحكي ال
+
+361
+00:29:14,720 --> 00:29:19,540
+total homoglobin ايش يعني؟ يعني ال homoglobin اللي
+
+362
+00:29:19,540 --> 00:29:24,620
+في كل خلايا الدم per microliter صح؟ تمام يعني انه
+
+363
+00:29:24,620 --> 00:29:29,060
+انا بنهاية كنت لما احصل على ال homoglobin كتركيز
+
+364
+00:29:29,060 --> 00:29:33,920
+كنت احصل عليه بوحدة gram per deciliter تمام؟ عدد
+
+365
+00:29:33,920 --> 00:29:39,380
+الخلايا بال deciliter تمام؟ كلها هذه انا أخدت ال
+
+366
+00:29:39,380 --> 00:29:46,700
+homoglobin اللي فيهاو طلعت وزنه طب أنا لو عارفة
+
+367
+00:29:46,700 --> 00:29:53,560
+الخلايا هذه عددها ككل مش ممكن يعني أقسم وزن ال ..
+
+368
+00:29:53,560 --> 00:29:57,960
+ال .. ال homoglobin ككل على عدد الخلايا ككل
+
+369
+00:29:57,960 --> 00:30:03,770
+بيطلعلي وزن ال homoglobin في الخلية الواحدةفإذا
+
+370
+00:30:03,770 --> 00:30:07,690
+هذا هو تفسير القانون اللي هو مجلبين ككل بال gram
+
+371
+00:30:07,690 --> 00:30:13,630
+per deciliter على ال RBCs count ككل و درب عشرة
+
+372
+00:30:13,630 --> 00:30:19,610
+طبعا فال normal range بيطلع من 28 ل 32 picogram
+
+373
+00:30:20,640 --> 00:30:26,180
+طبعا ال MCH نادرا أو يعني مش عادة بيستخدم لتصنيف
+
+374
+00:30:26,180 --> 00:30:30,540
+ال anemia ولكنه أحيانا بيكون مؤشر جيد عن محتوى ال
+
+375
+00:30:30,540 --> 00:30:36,080
+homoglobin في داخل ال RBCs كوزن تمام؟ هاي هنا مثال
+
+376
+00:30:36,080 --> 00:30:41,840
+كتطبيق للقانون ال homoglobin 16 ال RBCs 5 مليون
+
+377
+00:30:41,840 --> 00:30:47,270
+طبعا أنا باخد كام مليون؟ 5 خلاص بعود في الخمسةطيب
+
+378
+00:30:47,270 --> 00:30:51,330
+16 على 5 ده 14 بيطلع عندى طبعا 16 على 5 ثلاثة
+
+379
+00:30:51,330 --> 00:30:55,870
+point اتنين ال .. في عشرة اتنين و تلاتين picogram
+
+380
+00:30:55,870 --> 00:31:01,070
+تمام؟ و نفس ال issue إذا كان ال .. ال .. ال MCH
+
+381
+00:31:01,070 --> 00:31:08,510
+أقل من 28 it is low و إذا كان أكثر من 32 it's high
+
+382
+00:31:08,510 --> 00:31:14,150
+طيب؟ولكن احنا حكينا مش دايما انا بستخدموا فيه
+
+383
+00:31:14,150 --> 00:31:17,090
+classification للانيميا في تصنيف الانيميا او في
+
+384
+00:31:17,090 --> 00:31:21,750
+وصف الانيميا طيب ال mean carbocular homoglobin
+
+385
+00:31:21,750 --> 00:31:27,630
+concentration اللي هو ال MCHC هو عبارة عن متوسط
+
+386
+00:31:27,630 --> 00:31:32,030
+تمام ال homoglobin concentration متوسط تركيز ال
+
+387
+00:31:32,030 --> 00:31:35,150
+homoglobin في الكورية الواحدة او في الخلية الواحدة
+
+388
+00:31:35,460 --> 00:31:38,860
+فإذن هو عبارة عن the average concentration متوسط
+
+389
+00:31:38,860 --> 00:31:43,420
+التركيز لل homoglobin في كل خلية من خلايا ال .. ال
+
+390
+00:31:43,420 --> 00:31:48,500
+.. ال RBCs تمام؟ في الخلية الواحدة طيب فبن .. ال
+
+391
+00:31:48,500 --> 00:31:53,480
+.. وحدة القياس طبعا بتكون as a percentage أو ممكن
+
+392
+00:31:53,480 --> 00:31:57,840
+أن أنا أحكي عنها gram per ال deciliter تمام؟ طيب
+
+393
+00:31:59,450 --> 00:32:03,910
+طبعا ال .. ال MCHC قانونه ال homoglobin على ال
+
+394
+00:32:03,910 --> 00:32:08,810
+hematocrit ضارب 100% تمام؟ تركيز ال homoglobin ككل
+
+395
+00:32:08,810 --> 00:32:13,150
+على حجم اللي بتحتله ال RBCs فهنا بحصل على كام ال
+
+396
+00:32:13,150 --> 00:32:16,110
+.. النسبة ال .. ال homoglobin في الخلية الواحدة
+
+397
+00:32:16,110 --> 00:32:22,230
+تمام؟ فهي هنا مثال 16 ال homoglobin على 48 ال
+
+398
+00:32:22,230 --> 00:32:26,570
+hematocrit ضارب 100 فبيطلعلي 33.3 gram per
+
+399
+00:32:26,570 --> 00:32:35,210
+deciliter أو as a percentage حكي هناطيب ال ..
+
+400
+00:32:35,210 --> 00:32:38,430
+الخلية
+
+401
+00:32:38,430 --> 00:32:43,390
+الطبيعية بتحتوي على تركيز من الهموجلوبين ما بين 32
+
+402
+00:32:43,390 --> 00:32:48,870
+ل 37 gram per deciliter طبعا؟ طيب و هذه الخلية بما
+
+403
+00:32:48,870 --> 00:32:55,270
+أنها طبيعية بنصفها كأنها normal .. chromic طبعا؟
+
+404
+00:32:55,270 --> 00:33:00,760
+normal يعني normalو chromic لون، إذا لونها طبيعي،
+
+405
+00:33:00,760 --> 00:33:04,180
+يعني ال homoglobin اللي أصلا بيعطي ال RBC's اللون
+
+406
+00:33:04,180 --> 00:33:08,320
+طبيعي، تمام؟ تركيزوا طبيعي في الخلية طيب، بس هنا
+
+407
+00:33:08,320 --> 00:33:12,400
+جادير بتذكر إنه هنا بنوّهلي إنه زمان كانوا يعبروا
+
+408
+00:33:12,400 --> 00:33:18,020
+عن ال MCHC as a percentage و حاليا الوحدة الدرجة
+
+409
+00:33:18,020 --> 00:33:23,510
+هي gram per deciliter، تمام؟طيب hypochromic إيش
+
+410
+00:33:23,510 --> 00:33:26,950
+يعني hypochromic؟ hypo قليل، chromic لون، إذا
+
+411
+00:33:26,950 --> 00:33:30,670
+التركيز الهوموجلوبين هيكون أقل من 32 gram per
+
+412
+00:33:30,670 --> 00:33:35,710
+deciliter طيب hyper، hyper عالي، chromic لون،
+
+413
+00:33:35,710 --> 00:33:39,390
+hypochromic معناها تركيز الهوموجلوبين أعلى من 37
+
+414
+00:33:39,390 --> 00:33:44,220
+gram per deciliter، تمام؟ طيبالـ hypochromic
+
+415
+00:33:44,220 --> 00:33:47,720
+erythrocyte occur in thalassemia and iron
+
+416
+00:33:47,720 --> 00:33:51,200
+deficiency anemia و هذا الاشي صار بديهي بالنسبة
+
+417
+00:33:51,200 --> 00:33:53,660
+لإن حكينا إن ال thalassemia و ال iron deficiency
+
+418
+00:33:53,660 --> 00:33:58,520
+anemia بيكون فيهم الخلايا microcytic صغيرة و عرفنا
+
+419
+00:33:58,520 --> 00:34:02,640
+ليش صغيرة تمام؟ و بتكون أيضا hypochromicويعرفنا
+
+420
+00:34:02,640 --> 00:34:05,520
+ليش؟ لإن العلة أصلا في تصنيع ال homoglobin إما
+
+421
+00:34:05,520 --> 00:34:08,800
+مافيش ironic فاية أو في خلل أو قلة في تصنيع ال
+
+422
+00:34:08,800 --> 00:34:12,700
+homoglobin تمام؟ فإذا هتكون بالإضافة لإن هتكون
+
+423
+00:34:12,700 --> 00:34:16,360
+microcytic بسبب ال asynchronous في انقسام الخلية
+
+424
+00:34:16,360 --> 00:34:22,040
+هتكون أيضا hypoachromicطيب شوفوا معايا، هلأ إحنا
+
+425
+00:34:22,040 --> 00:34:26,960
+حكينا إنه في عندي level معين لل homoglobin إنه
+
+426
+00:34:26,960 --> 00:34:31,260
+الخلية لما بتصل لعنده بتتخرج من ال bone marrow و
+
+427
+00:34:31,260 --> 00:34:33,580
+بتطلع على ال circulation هذا ال level اللي هو ما
+
+428
+00:34:33,580 --> 00:34:40,520
+بين 32 ل 37 gram per deciliter، تمام؟ يعني مستحيل
+
+429
+00:34:40,520 --> 00:34:44,460
+الخلية تصل أعلى من هيكإذا مافيش عندي حاجة اسمها
+
+430
+00:34:44,460 --> 00:34:48,060
+hyperchromic طبعا مافيش خلية بيتم إنتاجها ك
+
+431
+00:34:48,060 --> 00:34:51,460
+hyperchromic يعني فيها يعني بتحتوي على homoglobin
+
+432
+00:34:51,460 --> 00:34:56,000
+concentration أعلى من 37 gram per deciliter طيب
+
+433
+00:34:56,000 --> 00:35:00,080
+إيش يعني؟ طب ليش أمتى بنستخدم hyperchromic؟
+
+434
+00:35:00,080 --> 00:35:05,660
+hyperchromic لما بيتغير شكل الخلية وبيتغير حجمها
+
+435
+00:35:05,850 --> 00:35:10,670
+فأيضا بيتغير توزيع ال homoglobin فيها فبنسميها ..
+
+436
+00:35:10,670 --> 00:35:13,390
+بتبطل by concave و discotheque بتصير spherical
+
+437
+00:35:13,390 --> 00:35:18,610
+بتصير زي الكرة تمام مدورة من كل الجهات فعايش ال ..
+
+438
+00:35:18,610 --> 00:35:23,740
+ال .. في هاي الحالة بتبين كأنها fullيعني حتى كمان
+
+439
+00:35:23,740 --> 00:35:27,220
+لما بشوفها على ال blood film مابشوفش منطقة ال
+
+440
+00:35:27,220 --> 00:35:30,100
+central baller بشوف ان ال homoglobin مغطيها كلها
+
+441
+00:35:30,100 --> 00:35:34,700
+تمام فهذه الحالة باصفها كوصف انها hyperchromic
+
+442
+00:35:34,700 --> 00:35:38,360
+ولكن لازم اعرف انه هي يعني ال homoglobin
+
+443
+00:35:38,360 --> 00:35:42,980
+concentration فيها لا يتجاوز ال 37 gram per
+
+444
+00:35:42,980 --> 00:35:49,280
+deciliter وهذه المعلومة احنا ذكرناهافي محاضرة ل
+
+445
+00:35:49,280 --> 00:35:55,200
+blood film حكينا إن ال RBCs بتكون طبعا فيها منطقة
+
+446
+00:35:55,200 --> 00:35:59,400
+تلتها في المركز بيكون في central pallor شحوب مركزي
+
+447
+00:35:59,400 --> 00:36:04,580
+منطقة خالية من ال homoglobin لو كانت الخلية طبعا
+
+448
+00:36:04,580 --> 00:36:07,620
+ما .. لا تحتوي على central pallor إذا هي
+
+449
+00:36:07,620 --> 00:36:13,120
+spherocytic تمام؟وإذا هي hyperchromic تجاوزا بصفها
+
+450
+00:36:13,120 --> 00:36:17,220
+ك hyperchromic لإنها it's as big as full تظهر
+
+451
+00:36:17,220 --> 00:36:21,020
+كإنها مليانة هموجلبين ولكن فعليا لا يتجاوز ل 37
+
+452
+00:36:21,020 --> 00:36:27,560
+gram per deciliter طبعا فإذا حسبنا MCHC أعلى من 37
+
+453
+00:36:27,560 --> 00:36:31,700
+gram per deciliter فهذا معناها إنه الهموجلبين
+
+454
+00:36:31,700 --> 00:36:37,020
+value تركيز الهموجلبين هناالهموجلبين قلي .. عالي
+
+455
+00:36:37,020 --> 00:36:40,940
+طبعا في error عالي البسط عالي يعني ال mchc هيطلع
+
+456
+00:36:40,940 --> 00:36:47,100
+عالي طبعا فمعناها في عندى error في الهموجلبين
+
+457
+00:36:47,100 --> 00:36:55,480
+value طيب اذا mcv normal mchc normal طبعا un يعني
+
+458
+00:36:55,480 --> 00:37:07,190
+normal اذا هي normocyticnormal normal normal
+
+459
+00:37:07,190 --> 00:37:07,250
+normal normal normal normal normal normal normal
+
+460
+00:37:07,250 --> 00:37:07,250
+normal normal normal normal normal normal normal
+
+461
+00:37:07,250 --> 00:37:07,830
+normal normal normal normal normal normal normal
+
+462
+00:37:07,830 --> 00:37:07,830
+normal normal normal normal normal normal normal
+
+463
+00:37:07,830 --> 00:37:07,830
+normal normal normal normal normal normal normal
+
+464
+00:37:07,830 --> 00:37:07,830
+normal normal normal normal normal normal normal
+
+465
+00:37:07,830 --> 00:37:07,830
+normal normal normal normal normal normal normal
+
+466
+00:37:07,830 --> 00:37:08,110
+normal normal normal normal normal normal normal
+
+467
+00:37:08,110 --> 00:37:08,430
+normal normal normal normal normal normal normal
+
+468
+00:37:08,430 --> 00:37:10,370
+normal normal normal normal normal normal normal
+
+469
+00:37:10,370 --> 00:37:18,630
+normal normal normal normal normal
+
+470
+00:37:18,630 --> 00:37:18,670
+normal normal normal normal normal normal normal
+
+471
+00:37:18,670 --> 00:37:18,750
+normal normal normal normal normal normal normal
+
+472
+00:37:18,750 --> 00:37:18,790
+normal normal normal normal normal normal normal
+
+473
+00:37:18,790 --> 00:37:18,790
+normal normal normal normal normal normal normal
+
+474
+00:37:18,790 --> 00:37:18,790
+normal normal normal normal normal normal normal
+
+475
+00:37:18,790 --> 00:37:18,790
+normal normal normal normal normal normal normal
+
+476
+00:37:18,790 --> 00:37:18,790
+normal normal normal normal normal normal normal
+
+477
+00:37:18,790 --> 00:37:18,790
+normal normal normal normal normal normal normal
+
+478
+00:37:18,790 --> 00:37:18,790
+normal normal normal normal normal normal normal
+
+479
+00:37:18,790 --> 00:37:18,790
+normal normal normal normal normal normal normal
+
+480
+00:37:18,790 --> 00:37:18,790
+normal normal normal normal normal normal normal
+
+481
+00:37:18,790 --> 00:37:18,790
+normal normal normal normal normal normal normal
+
+482
+00:37:18,790 --> 00:37:18,790
+normal normal normal normal normal normal normal
+
+483
+00:37:18,790 --> 00:37:18,790
+normal normal normal normal normal normal normal
+
+484
+00:37:18,790 --> 00:37:18,790
+normal normal normal normal normal normal normal
+
+485
+00:37:18,790 --> 00:37:18,790
+normal normal normal normal normal normal normal
+
+486
+00:37:18,790 --> 00:37:18,790
+normal normal normal normal normal normal normal
+
+487
+00:37:18,790 --> 00:37:18,790
+normal normal normal normal normal normal normal
+
+488
+00:37:18,790 --> 00:37:18,790
+normal normal normal normal normal normal normal
+
+489
+00:37:18,790 --> 00:37:18,790
+normal normal normal normal normal normal normal
+
+490
+00:37:18,790 --> 00:37:21,710
+normal normal normal normal normalطيب لو كان في
+
+491
+00:37:21,710 --> 00:37:24,930
+عندي decrease في التنتين إيش بدي أوصف ال anemia؟
+
+492
+00:37:24,930 --> 00:37:28,390
+بدي أحكي عن إنه قلة في ال MCV أو قلة في الحجم أو
+
+493
+00:37:28,390 --> 00:37:35,310
+صغر في الحجم يعني decrease MCV microcytic حكينا ب
+
+494
+00:37:35,310 --> 00:37:41,000
+.. بعبّر عنها كإنها microcytic بستخدم هذا اللفظطيب
+
+495
+00:37:41,000 --> 00:37:45,220
+قلة في اللون او يعني hemoglobin concentration قليل
+
+496
+00:37:45,220 --> 00:37:48,740
+ايضا decrease طبعا دي يعني decrease اذا
+
+497
+00:37:48,740 --> 00:37:52,900
+hypochromic حكينا اذا بسميها microcytic
+
+498
+00:37:52,900 --> 00:37:56,460
+hypochromic anemia ومثال على ال microcytic
+
+499
+00:37:56,460 --> 00:37:59,820
+hypochromic anemia اللي هي اما iron deficiency
+
+500
+00:37:59,820 --> 00:38:05,420
+anemia او ثلاثيميا طبعا طيب macrocytic
+
+501
+00:38:06,390 --> 00:38:12,250
+يعني إيش؟ ال MCV عالي increase تمام؟ و
+
+502
+00:38:12,250 --> 00:38:18,470
+normochromic يعني ال MCHC normal تمام؟ فهذا معنى
+
+503
+00:38:18,470 --> 00:38:21,470
+إنه الخلية أو الأنينيا عندي macrocytic
+
+504
+00:38:21,470 --> 00:38:26,670
+normochromic طبعا في عندي أسماء من ال .. ال .. أو
+
+505
+00:38:26,670 --> 00:38:32,110
+أنواع من الأنينيات اللي بيندرجوا تحت هاي ال ..
+
+506
+00:38:32,110 --> 00:38:39,140
+الوصف تحت هدول الأوصافتمام؟ فبالتالي الخطوة الأولى
+
+507
+00:38:39,140 --> 00:38:49,140
+إن أنا أقيم شكل ال .. ال .. ال RBCs و أشوف مؤشرات
+
+508
+00:38:49,140 --> 00:38:55,940
+ال RBCs هذه تمام؟أوصف نوع الأنيميا فبالتالي
+
+509
+00:38:55,940 --> 00:38:59,660
+بتحصرلي أنواع معينة مثلا normocytic-normochromic
+
+510
+00:38:59,660 --> 00:39:03,520
+في عندي أنواع معينة واحد اتنين تلاتة فبضل .. خلاص
+
+511
+00:39:03,520 --> 00:39:07,800
+بنحصر بي هدول الأنواع ببدأ أعمل احوصات تانية تفرق
+
+512
+00:39:07,800 --> 00:39:10,860
+ما بين الأنواع تبعوني normocytic-normochromic
+
+513
+00:39:10,860 --> 00:39:18,640
+تمام؟ كانت مؤشرات وشكل ال .. ال RBCsو ال indices
+
+514
+00:39:18,640 --> 00:39:22,640
+اللي انا حسبتهم بيحكولي انها microcytic
+
+515
+00:39:22,640 --> 00:39:25,860
+hypochromic اذا انا هتوجه اما هتكون ثلاثيميا او
+
+516
+00:39:25,860 --> 00:39:30,200
+iron deficiency anemia ممكن اعمل فحوصات لمستوى ال
+
+517
+00:39:30,200 --> 00:39:34,160
+iron ممكن يعني اعمل فحوصات لحتى اكشف عن جيناتي ال
+
+518
+00:39:34,160 --> 00:39:37,400
+homoglobin و اشوف اذا فيها خلل عشان اشخص ثلاثيميا
+
+519
+00:39:37,400 --> 00:39:43,320
+يعني فبالتالي اكون محصورة بالانواع اللي هي
+
+520
+00:39:43,320 --> 00:39:46,820
+microcytic hypochromicطمام؟ macrocyclic
+
+521
+00:39:46,820 --> 00:39:50,480
+-normochromic برضه كمان نفس الأشياء فبالتالي إذا
+
+522
+00:39:50,480 --> 00:39:54,720
+حكينا إنها بتساعدني في وصف نوع الأنيميا و ال
+
+523
+00:39:54,720 --> 00:39:57,840
+classification و ال differential diagnosis التفريق
+
+524
+00:39:57,840 --> 00:40:06,790
+ما بين هاي الأنيمياتتمام؟ طيب هاي مصطلحات راح ان
+
+525
+00:40:06,790 --> 00:40:12,590
+نذكرها already صيرنا بنعرفها بس خلوني بس أحكي
+
+526
+00:40:12,590 --> 00:40:20,880
+نقطتين أول إشي ال ..بنهاية المحاضرة هاي احنا
+
+527
+00:40:20,880 --> 00:40:27,400
+تقريبا صرنا بنعرف نشتغل و بنعرف نحسب كل بنود ال
+
+528
+00:40:27,400 --> 00:40:31,160
+CBC اللي هو ال complete blood count تمام؟ ال
+
+529
+00:40:31,160 --> 00:40:36,680
+complete blood count بيكون فيه عد ل ال YPCs و
+
+530
+00:40:36,680 --> 00:40:41,060
+بيكون فيه عد ل ال RPCs و احنا اتعرفناهم و يعرفنا
+
+531
+00:40:41,060 --> 00:40:48,500
+كيف بنشتغلهم و كيف بنحسبهم manual تمام؟و أيضًا
+
+532
+00:40:48,500 --> 00:40:53,200
+بيكون فيه differential ل ال white pieces يعني كام
+
+533
+00:40:53,200 --> 00:40:55,960
+نسبة ال monocyte، كام نسبة ال lymphocyte، ال
+
+534
+00:40:55,960 --> 00:40:59,420
+neutrophil، ال eosinophil، ال basophil، تمام،
+
+535
+00:40:59,420 --> 00:41:04,280
+فبرضه هذه أنا أعطيتكم فكرة عنها إنه إحنا من خلال
+
+536
+00:41:04,280 --> 00:41:09,280
+blood film نحسب، يعني بنعيد 100 خلية و بنشوف كام
+
+537
+00:41:09,280 --> 00:41:11,680
+طلع معنا منهم ال neutrophil، كام طلع معنا منهم
+
+538
+00:41:11,680 --> 00:41:16,670
+monocyte، و كام lymphocyteلأخرين و بنحسب نسبة
+
+539
+00:41:16,670 --> 00:41:21,970
+المئوية لإنهم تمام طيب و أيضا بيكون في حساب لل
+
+540
+00:41:21,970 --> 00:41:25,010
+homoglobin concentration و سرنا بنعرف بطريقة
+
+541
+00:41:25,010 --> 00:41:30,790
+draken method بنعرف نحسب تركيز ال homoglobin برضه
+
+542
+00:41:30,790 --> 00:41:36,830
+manual يدويوبيكون في حساب لتركيز او عدد ال
+
+543
+00:41:36,830 --> 00:41:40,390
+bloodlet الصفائح الدموية من خلال blood film يمكن
+
+544
+00:41:40,390 --> 00:41:46,390
+عدها ايضا فإذا احنا صيرنا بنعرف تقريبا نعمل CBC
+
+545
+00:41:46,390 --> 00:41:51,070
+يدوي صح؟ و بس مسألة ال difference يعني لو عدت ال
+
+546
+00:41:51,070 --> 00:41:54,850
+bloodlet هتاخدوها اكثر بشكل موسع في ال hematology
+
+547
+00:41:56,980 --> 00:42:02,440
+اللي بيفرق انه ال CPC بيكون automated جهاز بيشتغله
+
+548
+00:42:02,440 --> 00:42:05,820
+تمام؟ ولكن احنا بنفرق عن الجهاز يعني انا في
+
+549
+00:42:05,820 --> 00:42:09,340
+النهاية ممكن الجهاز يغلط لكن انا لازم اكون فاهمة و
+
+550
+00:42:09,340 --> 00:42:13,080
+عارفة المبدأ او من وين اجت الحسابات و كيف الجهاز
+
+551
+00:42:13,080 --> 00:42:16,380
+اصلا بيعد و كيف بيشتغل فدي كلها لازم نكون احنا
+
+552
+00:42:16,380 --> 00:42:19,940
+بنعرفها عشان هيك احنا اتعلمناالطريقة اليدوية
+
+553
+00:42:19,940 --> 00:42:24,520
+الجهاز بيستخدم المعادلات هاي لحتى يحسب ال indices
+
+554
+00:42:24,520 --> 00:42:29,060
+و بيطلعلي mcb و mch و mchc و هيكم انتوا اتعلمتوا
+
+555
+00:42:29,060 --> 00:42:34,820
+كمان اليوم ان كيف تحسبوهم فبالتالي اذا يعني صار في
+
+556
+00:42:34,820 --> 00:42:38,780
+عندنا رؤية واضحة انا كيف ممكن اعمل complete blood
+
+557
+00:42:38,780 --> 00:42:41,000
+count لشخص يدويا
+
+558
+00:42:43,450 --> 00:42:47,050
+طيب خلّينا نشوف إيش يعني anemia مرت معانا إنه هي
+
+559
+00:42:47,050 --> 00:42:50,490
+عبارة عن conditions، ظروف، variety of condition
+
+560
+00:42:50,490 --> 00:42:55,750
+طبعا، متنوعة هذه الظروف المحصلة إن ال person's
+
+561
+00:42:55,750 --> 00:42:59,350
+blood دم الشخص can't carry as much oxygen as it
+
+562
+00:42:59,350 --> 00:43:03,870
+should يعني مابيحملش oxygen كما ينبغي اللي إما
+
+563
+00:43:03,870 --> 00:43:08,330
+بيكون سبب إنه ال number أو ال size أو ال
+
+564
+00:43:08,330 --> 00:43:15,130
+homoglobin content لل RBCs قليل طبعاوالحالة يعني
+
+565
+00:43:15,130 --> 00:43:21,150
+بيكون المحصلة hypoxia تمام hypochromic حكينا hypo
+
+566
+00:43:21,150 --> 00:43:26,470
+نقصان أو قلة في ash اللون يعني قلة في تركيز ال
+
+567
+00:43:26,470 --> 00:43:31,490
+homoglobin إذا هو المصطلح اللي بيطبق على ال RBCs
+
+568
+00:43:31,490 --> 00:43:34,490
+لما بتكون decreased concentration of homoglobin
+
+569
+00:43:34,490 --> 00:43:39,090
+عندها نقص في تركيز ال homoglobinطبعا macrocytic
+
+570
+00:43:39,090 --> 00:43:43,330
+يعني خلية اش macro كبيرة, cytic خلية كبيرة, اذا
+
+571
+00:43:43,330 --> 00:43:47,370
+larger than normal red blood cell, main carboxylic
+
+572
+00:43:47,370 --> 00:43:51,590
+homoglobin هو ال average homoglobin طبعا weight,
+
+573
+00:43:51,910 --> 00:43:55,270
+وزن ال homoglobin في ال RBCs, ال main carboxylic
+
+574
+00:43:55,270 --> 00:43:57,150
+homoglobin concentration هو ال average
+
+575
+00:43:57,150 --> 00:44:01,110
+concentrationللهموجلبين في ال red blood cell ال
+
+576
+00:44:01,110 --> 00:44:03,990
+main corpuscular volume هو الأذرش volume لل red
+
+577
+00:44:03,990 --> 00:44:09,190
+blood cell طبعا micro حكيمه صغير و 6 خلية إذا خلية
+
+578
+00:44:09,190 --> 00:44:12,910
+smaller than normal ال MCV تبعها هيكون قليل
+
+579
+00:44:12,910 --> 00:44:16,970
+normochromic خلية اللون فيها طبيعي إذا ال red
+
+580
+00:44:16,970 --> 00:44:19,490
+blood cell عندها normal concentration of
+
+581
+00:44:19,490 --> 00:44:24,310
+homoglobinnormocytic يعني خلية حجمها طبيعي إذا ال
+
+582
+00:44:24,310 --> 00:44:28,390
+red blood cell normal size ال MCV normal و ال red
+
+583
+00:44:28,390 --> 00:44:32,930
+blood cell indices كشكل يعني كشكل عام إنها عبارة
+
+584
+00:44:32,930 --> 00:44:36,890
+عن المؤشرات والقياسات اللي بتفسر الحجم و ال
+
+585
+00:44:36,890 --> 00:44:40,030
+homoglobin content لل red blood cell تمام؟ و هذا
+
+586
+00:44:40,030 --> 00:44:44,010
+هو التعريف اللي إحنا بدأنا في محاضرتنا عم نختم فيه
+
+587
+00:44:44,010 --> 00:44:48,350
+هلأ و هيك بنكون وصلنا لنهاية المحاضرة يعطيكم ألف
+
+588
+00:44:48,350 --> 00:44:48,770
+عافية
+
diff --git a/PL9fwy3NUQKwY9jl8xw-bWkVZkWcGGyspd/Vo0zfcjPiBQ.srt b/PL9fwy3NUQKwY9jl8xw-bWkVZkWcGGyspd/Vo0zfcjPiBQ.srt
new file mode 100644
index 0000000000000000000000000000000000000000..c6b83265793d54e41d29a88becb2d8d671963abe
--- /dev/null
+++ b/PL9fwy3NUQKwY9jl8xw-bWkVZkWcGGyspd/Vo0zfcjPiBQ.srt
@@ -0,0 +1,2654 @@
+1
+00:00:04,520 --> 00:00:07,580
+السلام عليكم ورحمة الله وبركاته، طالبتي اليوم إن
+
+2
+00:00:07,580 --> 00:00:12,060
+شاء الله، رح نأخذ معملنا رقم اثنين بعنوان automatic
+
+3
+00:00:12,060 --> 00:00:15,600
+pipette and calibration. معمل رقم اثنين فيه مساق
+
+4
+00:00:15,600 --> 00:00:20,760
+مقدمة في التحليل الطبي. طبعاً هذا كان أول موضوع كان
+
+5
+00:00:20,760 --> 00:00:25,100
+المفروض أن نحنا نأخذه عملياً، ونشتغل بأدواته، ولكن
+
+6
+00:00:25,100 --> 00:00:30,440
+للأسف، نظراً للظروف المحيطة، فنحن راح نأخذ إن شاء الله
+
+7
+00:00:30,440 --> 00:00:33,660
+...راح نأخذ النظري تبعه، وإن شاء الله بعودتنا
+
+8
+00:00:33,660 --> 00:00:38,460
+للمختبرات نأخذ التطبيق العملي له. فاليوم إن شاء
+
+9
+00:00:38,460 --> 00:00:41,060
+الله موضوعنا عن الـ automatic pipette and
+
+10
+00:00:41,060 --> 00:00:44,280
+calibration. خلينا نشوف إيش يعني automatic pipette
+
+11
+00:00:44,280 --> 00:00:47,480
+هلأ، احنا المحاضرة الماضية، خلينا بس نحكي هالنقطة.
+
+12
+00:00:47,790 --> 00:00:53,150
+المحاضرة الماضية كانت بتحكي عن الـ ... الـ ... الـ
+
+13
+00:00:53,150 --> 00:00:58,270
+volumes والحجوم والمحاليل، وكيف أنا بتعامل مع
+
+14
+00:00:58,270 --> 00:01:01,110
+المحاليل هذه، وإيش أنواع المحاليل اللي تستخدم في
+
+15
+00:01:01,110 --> 00:01:05,930
+الـ medical laboratories، و... وكيف أنا أُخفف هذه
+
+16
+00:01:05,930 --> 00:01:10,690
+المحاليل، تمام؟ طيب، لما أنا ... أنا بدي أجي أنقل
+
+17
+00:01:10,690 --> 00:01:13,950
+المحلول من إلى، يعني مثلاً أنا عندي عينة بدي
+
+18
+00:01:13,950 --> 00:01:17,970
+أضيف لها reagent علشان أعمل فحص معين، أو بدي آخذ
+
+19
+00:01:17,970 --> 00:01:22,130
+... بدي أنقل حجوم علشان أعمل dilution زي ما أخذنا
+
+20
+00:01:22,130 --> 00:01:25,800
+الـ... مسائل الـ dilution المحاضرة الماضية. كيف أنا
+
+21
+00:01:25,800 --> 00:01:29,140
+بدي أنقلها؟ هنا بالـ medical laboratories
+
+22
+00:01:29,140 --> 00:01:32,660
+بالمختبرات الطبية، احنا بنستخدم شيء اسمه automatic
+
+23
+00:01:32,660 --> 00:01:37,100
+pipette، اللي هو الأداة اللي موجودة بهذه الصورة. اسمها
+
+24
+00:01:37,100 --> 00:01:40,740
+automatic pipette. خلينا نشوف إيش يعني automatic pipette.
+
+25
+00:01:41,150 --> 00:01:45,250
+هلأ، automatic pipette هي عبارة عن أداة تستخدم في الـ
+
+26
+00:01:45,250 --> 00:01:48,110
+biological والـ biochemical والـ microbiological
+
+27
+00:01:48,110 --> 00:01:52,310
+labs، سواء كانت للـ teaching purposes أو للـ research
+
+28
+00:01:52,310 --> 00:01:56,490
+purposes، تمام؟ يعني تستخدم في مختبرات الأحياء، و
+
+29
+00:01:56,490 --> 00:01:59,750
+مختبرات الـ biochemistry والـ microbiology في كل
+
+30
+00:01:59,750 --> 00:02:04,700
+أقسام المختبرات الطبية، وتستخدم إما للتدريس أو حتى
+
+31
+00:02:04,700 --> 00:02:09,640
+للعمل، لممارسة المهنة، أو لأغراض البحث العلمي. طبعاً
+
+32
+00:02:09,640 --> 00:02:13,700
+من شأن هذه الـ pipette أنها تعملي accurate transfer of
+
+33
+00:02:13,700 --> 00:02:18,500
+volume، يعني أنها تنقل الحجوم بشكل دقيق جداً، بمثابة
+
+34
+00:02:18,500 --> 00:02:22,050
+الكيمياء. يمكن أخذتم معامل كيمياء قبل هيك، كنتم
+
+35
+00:02:22,050 --> 00:02:25,250
+تستخدموا pipettes اللي هي زي الـ glass pipette اللي كان
+
+36
+00:02:25,250 --> 00:02:29,490
+عبارة عن pipette زجاجية، وفي آخرها، أو في
+
+37
+00:02:29,490 --> 00:02:33,210
+نهايتها بيكون في عندي مستودع من المطاط، أنا بتحكم
+
+38
+00:02:33,210 --> 00:02:37,160
+بدخول وخروج الهواء، ودخول وخروج السائل. إلى الـ pipette
+
+39
+00:02:37,160 --> 00:02:41,280
+طبعاً هذه الـ glass pipette بتستخدم بالـ chemical
+
+40
+00:02:41,280 --> 00:02:45,380
+laboratory، ولكن للأسف هي not accurate و not
+
+41
+00:02:45,380 --> 00:02:49,820
+precise. مش accurate ومش precise، متى؟ طبعاً لما بدي
+
+42
+00:02:49,820 --> 00:02:53,640
+أنقل حجوم أقل من one ml، واحنا هنا بالمختبرات
+
+43
+00:02:53,640 --> 00:02:57,000
+الطبية بنتعامل دائماً مع حجوم صغيرة جداً بالـ
+
+44
+00:02:57,000 --> 00:03:01,970
+microliter، يعني أقل من one ml. فبالتالي، أنا بدي أداة
+
+45
+00:03:01,970 --> 00:03:07,230
+تنقل الحجم هذا الصغير، أو الحجم القليل، بطريقة more
+
+46
+00:03:07,230 --> 00:03:11,170
+accurate and more precise، how؟ باستخدام الـ
+
+47
+00:03:11,170 --> 00:03:15,470
+automatic pipette. إذا الـ glass pipette هي تستخدم بالـ
+
+48
+00:03:15,470 --> 00:03:19,670
+chemical laboratory، ولكن للأسف في الحجوم الصغيرة
+
+49
+00:03:19,670 --> 00:03:25,060
+not accurate، not precise. أنا محتاجة أداة تكون أكثر
+
+50
+00:03:25,060 --> 00:03:28,560
+دقة، more accurate، more precise. لهيك أنا بستخدم الـ
+
+51
+00:03:28,560 --> 00:03:32,200
+automatic pipette، لأن كل شغلي بيكون على الـ volumes
+
+52
+00:03:32,200 --> 00:03:37,480
+اللي أقل من one ml. طبعاً، طيب، كل pipette من هذول الـ
+
+53
+00:03:37,480 --> 00:03:42,440
+automatic pipettes مصممة إنها تنقل، يعني مدى، أو
+
+54
+00:03:42,440 --> 00:03:47,050
+range من الحجوم، من إلى. يعني في pipette متكون مصممة
+
+55
+00:03:47,050 --> 00:03:50,930
+تنقل من واحد microliter لعشرة microliters، في من...
+
+56
+00:03:50,930 --> 00:03:54,770
+مصممة بتكون من عشرة microliters لمئة microliters، في من...
+
+57
+00:03:54,770 --> 00:03:58,610
+من مئة microliters لـ one ml. تمام؟ ف... وفي من one
+
+58
+00:03:58,610 --> 00:04:04,050
+ml لخمسة ml. فإذا الـ... في pipettes أنواع وحجوم، و
+
+59
+00:04:04,050 --> 00:04:08,390
+كل pipette بتكون إلها range، يعني مدى من إلى.
+
+60
+00:04:08,390 --> 00:04:15,140
+بتستخدموا في الـ... الـ... الـ... نقل الحجوم هذه، و
+
+61
+00:04:15,140 --> 00:04:19,600
+طبعاً احنا المفروض أننا ما نتخطى هذا الـ... الـ... الـ
+
+62
+00:04:19,600 --> 00:04:23,820
+range تبعها. يعني لازم أنا، مثلاً، إذا بدي أنقل خمسين
+
+63
+00:04:23,820 --> 00:04:28,320
+microliter، يفضل أن أنا آخذ pipette الـ range تبعها
+
+64
+00:04:28,320 --> 00:04:31,480
+بحيث أن الخمسين تكون واقعة في الـ range، في داخل
+
+65
+00:04:31,480 --> 00:04:34,980
+هذا الـ range. يعني مثلاً، بأخذ pipette بتنقل من عشرة
+
+66
+00:04:34,980 --> 00:04:38,420
+microliters لمئة microliters، الـ volume رقم خمسين
+
+67
+00:04:38,420 --> 00:04:42,580
+هيكون already موجود، يعني في المدى، والـ pipette بتقدر
+
+68
+00:04:42,580 --> 00:04:46,880
+أنها تنقلي هذا الـ volume. فبالتالي، أنا هكون ضمنت الـ
+
+69
+00:04:46,880 --> 00:04:50,250
+accuracy والـ precision لنقل الـ volumes. طيب، إذا
+
+70
+00:04:50,250 --> 00:04:54,850
+احنا نوّهنا إنه أنا في عندي أنواع وحجوم، ويعني قدرة
+
+71
+00:04:54,850 --> 00:04:58,930
+استيعابية للـ pipettes، يعني مش كلها واحدة، مش كلها
+
+72
+00:04:58,930 --> 00:05:03,930
+مصممة إنها تنقل لي نفس الحجم. طيب، فإذا في عندي حاجة
+
+73
+00:05:03,930 --> 00:05:08,010
+اسمها pipette man model. طبعاً هي سبعة أنواع من الـ
+
+74
+00:05:08,010 --> 00:05:12,440
+pipettes، مصممة إنها تنقل حجوم بطريقة مختلفة. طبعاً
+
+75
+00:05:12,440 --> 00:05:17,560
+هذه الـ... الـ man model، يعني لو هم السبعة بيكونوا
+
+76
+00:05:17,560 --> 00:05:20,980
+بينقلوا لي من 0.1 microliter، يعني من أقل من
+
+77
+00:05:20,980 --> 00:05:24,680
+واحد microliter لحد خمسة آلاف microliter، يعني لحد
+
+78
+00:05:24,680 --> 00:05:30,100
+خمسة ml. يعني في تنوع من الـ pipettes هذه، وهلأ بنشوف
+
+79
+00:05:30,100 --> 00:05:35,090
+بالجدول اللي في الـ slide التالية، هيوضح لي إيش
+
+80
+00:05:35,090 --> 00:05:38,970
+يعني pipette مع الموديل. ولكن هنا بيحكي لنا إنه the
+
+81
+00:05:38,970 --> 00:05:42,710
+most often used، يعني الأكثر استخداماً هي الـ pipettes
+
+82
+00:05:42,710 --> 00:05:49,150
+اللي تسمى الـ B20 والـ B200 والـ B1000. هذول
+
+83
+00:05:49,150 --> 00:05:54,150
+الـ models هم الأكثر استخداماً، اللي بينقلوا لي حجم من
+
+84
+00:05:54,150 --> 00:05:58,450
+اثنين لعشرين، ومن خمسين لمئتين، ومن مئة لألف
+
+85
+00:05:58,450 --> 00:06:02,170
+microliter. تمام؟ هذه المدى تبعهم respectively، مرتب
+
+86
+00:06:02,170 --> 00:06:07,410
+بالترتيب. الـ B20 بتنقل من اثنين لعشرين microliter.
+
+87
+00:06:07,410 --> 00:06:12,990
+تمام؟ والـ pipette B200 بتنقل volume من خمسين لمئتين
+
+88
+00:06:12,990 --> 00:06:16,730
+microliter، والـ pipette B1000 بتأخذ من مئة لألف
+
+89
+00:06:16,730 --> 00:06:20,970
+microliter. يعني الرقم اللي جنب الـ B، تمام؟ يعني
+
+90
+00:06:20,970 --> 00:06:25,240
+pipette، والرقم هذا يعني أقصى قيمة بتقدر تأخذها. طبعاً
+
+91
+00:06:25,240 --> 00:06:28,800
+ومش... مش أدنى قيمة، لأ، في أدنى... الأدنى قيمة غير
+
+92
+00:06:28,800 --> 00:06:32,080
+مذكورة. هلأ، نيجي نشوف الـ pipette man model. هي في
+
+93
+00:06:32,080 --> 00:06:36,960
+عندي واحد، اثنين، أربعة، ستة، سبعة أنواع من الـ pipettes.
+
+94
+00:06:36,960 --> 00:06:43,870
+طبعاً، ومسمى بـ B2، يعني بتنقل من صفر لاثنين. وطبعاً الـ
+
+95
+00:06:43,870 --> 00:06:51,010
+B20 من صفر لعشرة. بتأخذ الـ B20 بتأخذ من صفر لعشرين. الـ B100
+
+96
+00:06:51,010 --> 00:06:55,250
+بتأخذ من صفر لمئة، و200 من صفر لمئتين، وهكذا، من صفر لـ
+
+97
+00:06:55,250 --> 00:07:00,570
+1000. يعني من ألف microliter، وهنا من صفر لخمسة آلاف، لحد one...
+
+98
+00:07:00,570 --> 00:07:04,310
+لحد خمسة ml. هذا الـ range، بصي، بالـ... اللي بيكون
+
+99
+00:07:04,310 --> 00:07:07,690
+مسجل على الـ pipette نفسها، يعني بيكون... بيكون...
+
+100
+00:07:07,690 --> 00:07:12,960
+بيعطيني الـ range إنه من... من كذا لكذا. أنا لما بدي
+
+101
+00:07:12,960 --> 00:07:16,040
+أستخدم الـ pipette هذه، طبعاً أنا ما بستخدم الـ pipette
+
+102
+00:07:16,040 --> 00:07:18,700
+عشان أنقل zero volume، يعني أنا ما بديش أنقل volume
+
+103
+00:07:18,700 --> 00:07:21,840
+ليش أستخدم الـ pipette؟ zero volume، يعني no volume.
+
+104
+00:07:21,840 --> 00:07:26,160
+طبعاً. فإذا في عندي حاجة اسمها recommended range.
+
+105
+00:07:26,160 --> 00:07:29,600
+يعني الـ range الموصى به، يعني أنا بستخدم الـ pipette
+
+106
+00:07:29,600 --> 00:07:36,260
+لحتى إنه أنا... يعني في range مسموح لي فيه. إيش هو اللي
+
+107
+00:07:36,260 --> 00:07:42,040
+مسموح لي فيه؟ من 0.1 لاثنين. وطبعاً الـ... و
+
+108
+00:07:42,040 --> 00:07:45,880
+هنا من نصف لعشرة، من اثنين لعشرين، من عشرة لمئة، و
+
+109
+00:07:45,880 --> 00:07:50,400
+هكذا، من خمسين لمئتين. طيب، هذه الـ pipette اللي من
+
+110
+00:07:50,400 --> 00:07:53,780
+خمسين لمئتين، ممكن تسحب لأقل من خمسين؟ اه، ممكن تسحب
+
+111
+00:07:53,780 --> 00:07:56,560
+لأقل من خمسين. الـ range تبعها أصلاً من صفر لمئتين.
+
+112
+00:07:56,900 --> 00:08:00,800
+ولكن هو إذا سحبت أقل من خمسين، فهي بتكون inaccurate
+
+113
+00:08:00,800 --> 00:08:05,200
+and imprecise. يعني مش accurate ومش precise. فأنا
+
+114
+00:08:05,200 --> 00:08:08,300
+يفضل أن أنا أستخ... أخت... يعني أتبع الـ
+
+115
+00:08:08,300 --> 00:08:13,460
+recommended range، علشان أستخ... أختار الـ... الـ...
+
+116
+00:08:13,460 --> 00:08:18,170
+الـ pipette المناسبة لي، تمام؟ طيب، يعني مثلاً لو أنا بدي
+
+117
+00:08:18,170 --> 00:08:23,030
+أنقل خمسين microliter، تمام؟ خمسين microliter، مين
+
+118
+00:08:23,030 --> 00:08:29,190
+أفضل أختار الـ... الـ pipette مثلاً رقم الـ B100 ولا الـ
+
+119
+00:08:29,190 --> 00:08:33,410
+B200؟ يفضل أن أنا أختار الـ B100، لإنه الخمسين
+
+120
+00:08:33,410 --> 00:08:36,570
+القيمة خمسين تقع في منتصف الـ range، يعني أنا ب... بكون
+
+121
+00:08:36,570 --> 00:08:41,710
+ضمن إنه ما فيش أخطاء. تمام؟ الـ pipette على الـ border،
+
+122
+00:08:41,710 --> 00:08:45,850
+على الحدود، تمام؟ ممكن يكون فيها نسبة خطأ، لكن في
+
+123
+00:08:45,850 --> 00:08:49,950
+منتصف الـ range بتكون دقيقة. فإذا أنا بفضل أن أنا
+
+124
+00:08:49,950 --> 00:08:54,810
+لما بدي اختار اللي بدي أنقل volume، بختار الـ pipette
+
+125
+00:08:54,810 --> 00:08:57,870
+اللي هذا الـ volume يتوسط الـ range تبعها، أو موجود
+
+126
+00:08:57,870 --> 00:09:02,120
+في الـ range تبعها. طبعاً، طيب، هلأ لو بدي أنقل مثلاً مئة
+
+127
+00:09:02,120 --> 00:09:08,300
+microliter، مين أفضل أختار؟ الـ B100 طبعاً، ولا الـ B200،
+
+128
+00:09:08,300 --> 00:09:15,620
+ولا الـ B1000؟ يفضل أن أنا أختار الـ B200 طبعاً، وممكن
+
+129
+00:09:15,620 --> 00:09:18,560
+أنا أختار الـ B100، بتكون دقيقة. يعني الـ higher range
+
+130
+00:09:18,560 --> 00:09:23,040
+تبعها بتكون أدق دائماً. طبعاً الـ higher range بتكون
+
+131
+00:09:23,040 --> 00:09:26,680
+أدق من الـ lower range للـ pipette. طبعاً، يعني هذه الـ
+
+132
+00:09:26,680 --> 00:09:32,120
+pipette الـ B100، يعني لو بدي أنقل 100 microliter من الـ
+
+133
+00:09:32,120 --> 00:09:37,620
+volume، ممكن أستخدم الـ B200، الـ... الـ... الـ B100،
+
+134
+00:09:37,620 --> 00:09:43,440
+ممكن أستخدم الـ B1000. تمام؟ و... لكن مثلاً هذه الـ pipette
+
+135
+00:09:43,440 --> 00:09:47,500
+اللي هو الـ range تبعها من عشرة لمئة. تمام؟ عند
+
+136
+00:09:47,500 --> 00:09:50,800
+المئة بتكون أدق من من... من... من أني أسحب فيها
+
+137
+00:09:50,800 --> 00:09:54,640
+عشرة microliters. يعني دائماً الـ higher limit تبعها
+
+138
+00:09:54,640 --> 00:09:57,990
+بتكون أدق من الـ lower limit للـ pipette نفسها. طبعاً
+
+139
+00:09:57,990 --> 00:10:02,750
+إذا، نرجع نعيد الصياغة ثانيةً، لو أنا في عندي... بدي
+
+140
+00:10:02,750 --> 00:10:05,690
+أنقل 100 microliter، مين أفضل أستخدم؟ الأفضل أن أنا
+
+141
+00:10:05,690 --> 00:10:09,990
+أستخدم الـ B200، لأن المئة تتوسط الـ range. ممكن
+
+142
+00:10:09,990 --> 00:10:13,730
+أستخدم الـ B100، لأن حكينا الـ higher limit بتكون
+
+143
+00:10:13,730 --> 00:10:16,990
+أدق دائماً من الـ lower limit للـ pipette، ولكن أنا
+
+144
+00:10:16,990 --> 00:10:20,110
+ما بستخدم... يعني ممكن أستخدمها، بس ما بكون دقيقة، و
+
+145
+00:10:20,110 --> 00:10:24,170
+... و... كثيراً، لأن دائماً حكينا إنه الـ lower limit
+
+146
+00:10:24,170 --> 00:10:27,370
+للـ pipette بتكون أقل دقة من الـ higher limit. هذا
+
+147
+00:10:27,370 --> 00:10:31,690
+بالنسبة لـ... الـ... الـ... الـ pipette man model. حكينا
+
+148
+00:10:31,690 --> 00:10:36,770
+مين هم أكثر شيوعاً، وأكثر استخداماً؟ بي twenty والـ
+
+149
+00:10:36,770 --> 00:10:41,910
+بي one hundred والـ بي two hundred. تمام؟ إذا الـ pipette
+
+150
+00:10:41,910 --> 00:10:46,570
+عشرين، الـ pipette مئة، والـ pipette مئتين هم أكثر الـ... الـ pipettes
+
+151
+00:10:46,570 --> 00:10:50,570
+شيوعاً واستخداماً. تمام؟ طيب، هلأ، بدنا نحكي عن
+
+152
+00:10:50,570 --> 00:10:56,150
+أنواع... يعني، sorry، مكونات الـ pipette وأجزاؤها. طيب،
+
+153
+00:10:56,150 --> 00:11:00,430
+الـ pipette زي ما أنتم شايفين بهذا الشكل، مكونة من حاجة
+
+154
+00:11:00,430 --> 00:11:05,030
+اسمها الـ... البش... البشطون، أو البلنجر، نحكي عنه
+
+155
+00:11:05,030 --> 00:11:09,770
+إما بشطون أو بلنجر. إيش يعني بشطون أو بلنجر؟ زي ما
+
+156
+00:11:09,770 --> 00:11:13,250
+أنتم شايفين، هذا الـ push button، هو عبارة عن كبسة
+
+157
+00:11:13,250 --> 00:11:18,830
+push، أنا ممكن أنا أضغط عليها، وتطلع وتنزل من الـ
+
+158
+00:11:18,830 --> 00:11:24,030
+pipette button. تمام؟ وهذا الـ... تبعها، طبعاً هذا الـ... الـ
+
+159
+00:11:24,030 --> 00:11:29,980
+... الـ... الـ... الـ... القضيب هذا، ممكن إنه يلتف دائرياً
+
+160
+00:11:29,980 --> 00:11:35,500
+علشان يتحكم في الـ volume. تمام؟ يتحكم في الـ volume
+
+161
+00:11:35,500 --> 00:11:39,620
+اللي موجودة على الـ digital screen هنا، تمام؟ هلأ في
+
+162
+00:11:39,620 --> 00:11:45,240
+هنا adapter موجود، هذا يعني علشان أنا أقلل من حركة
+
+163
+00:11:45,240 --> 00:11:47,800
+الـ pipette. يعني ممكن أنا أمسك هذا الـ adapter على
+
+164
+00:11:47,800 --> 00:11:51,880
+إصبعي... على إصبعي السبابة، وأمسكها بشكل إنه يكون
+
+165
+00:11:51,880 --> 00:11:58,230
+أنا متحكمة فيها، وأمسكها بشكل صحيح. هذا بسميه tip
+
+166
+00:11:58,230 --> 00:12:04,410
+ejector... tip ejector أو tip eject button. تمام؟ هو
+
+167
+00:1
+
+223
+00:16:29,140 --> 00:16:32,720
+بالـ bybit في المعامل أنا بأخذ الـ bybit .. بأخذ الطالبة
+
+224
+00:16:32,720 --> 00:16:37,120
+تمسك الـ bybit تمام، وبحيث أن الـ digital screen
+
+225
+00:16:37,120 --> 00:16:41,300
+تكون مواجهة لها تمام، يعني تكون عينها على الـ
+
+226
+00:16:41,300 --> 00:16:45,800
+digital screen وبالتالي بما أنه بيكون عينها على الـ
+
+227
+00:16:45,800 --> 00:16:49,660
+digital screen إذا حركت هذا الـ rod بشكل دائري
+
+228
+00:16:49,660 --> 00:16:54,260
+باتجاهها، يعني لعندها تمام، هيك بيزيد الـ volume أما
+
+229
+00:16:54,260 --> 00:16:59,180
+إذا حركته باتجاه المعاكس، يعني بعيد عنها، فهو بيقل
+
+230
+00:16:59,180 --> 00:17:02,340
+الـ volume تمام، هذا بده ممارسة، إن شاء الله بنشرحه
+
+231
+00:17:02,340 --> 00:17:09,020
+بالطريقة أو بالتجربة العملية، تمام؟ هذا بالنسبة لـ ..
+
+232
+00:17:09,020 --> 00:17:14,320
+الـ .. الـ .. الـ bybit و الطرق أو اللي هي الخطوات
+
+233
+00:17:14,320 --> 00:17:17,740
+أو اللي .. الـ .. التعليمات اللي لازم أنا ألتزم
+
+234
+00:17:17,740 --> 00:17:22,920
+فيها أثناء السحب أو نقل الحجوم باستخدام الـ bybit
+
+235
+00:17:23,850 --> 00:17:29,330
+في شغل هنا أن الـ .. الـ bybit هنا طبعاً هي بتكون
+
+236
+00:17:29,330 --> 00:17:33,210
+مصممة أنها تنقلي أو تقفز قفزات في الـ volume يعني
+
+237
+00:17:33,210 --> 00:17:38,970
+مثلاً هاي هنا بحكي لي أنه هي صح، يعني من خمسين لـ مئة
+
+238
+00:17:38,970 --> 00:17:45,210
+بتسحب لكن أنه مثلاً أنا لما بدي .. أحرك بصير بحرك
+
+239
+00:17:45,210 --> 00:17:48,450
+بيزيد الـ volume كل شوية point اتنين point اتنين
+
+240
+00:17:48,450 --> 00:17:51,890
+لحد ما أوصل للـ volume اللي بدي إياها، لذا هذه هي الـ ..
+
+241
+00:17:51,890 --> 00:17:55,450
+الـ .. مدى التحريك أو اللي هي القفزات في الـ
+
+242
+00:17:55,450 --> 00:18:01,330
+volumes طبعاً، طيب place a tip on the
+
+243
+00:18:01,330 --> 00:18:05,230
+discharge end of the pipet طبعاً أنا بـ .. بركب الـ
+
+244
+00:18:05,230 --> 00:18:10,370
+tip في المكان المخصص لها، وبعد هيك طبعاً بـ ..
+
+245
+00:18:12,600 --> 00:18:16,600
+باستخدام البلانجر بـ .. باستخدمه عشان أسحب الـ
+
+246
+00:18:16,600 --> 00:18:20,520
+volumes هذا البلانجر اللي إحنا شايفينه، تمام، مش ..
+
+247
+00:18:20,520 --> 00:18:24,180
+.. أيضاً هو يعني فائـد .. فائدته مش بس أنه متصل
+
+248
+00:18:24,180 --> 00:18:28,360
+بالـ rod، بالـ .. وبتحكم بالـ volume لأ، هذا
+
+249
+00:18:28,360 --> 00:18:31,600
+البلانجر إحنا بنسميه push button يعني أنا ممكن أكبس
+
+250
+00:18:31,600 --> 00:18:35,480
+عليه، بيطلع وبينزل، طبعاً أنا هذا لما بدي أكبس عليه و
+
+251
+00:18:35,480 --> 00:18:39,860
+يطلع وينزل، بينزل عند ضغطتين، الضغطة الأولى لما
+
+252
+00:18:39,860 --> 00:18:43,840
+أضغط الضغطة الأولى بيقل لي الـ exact volume اللي
+
+253
+00:18:43,840 --> 00:18:50,720
+موجود على الشاشة، تمام؟ لما أضغط ضغطتين، تمام؟ بينقل لي
+
+254
+00:18:50,720 --> 00:18:54,280
+extra volume ممكن يكون حجم مضاعف أو extra volume
+
+255
+00:18:54,280 --> 00:18:59,560
+من الـ volume اللي مصمم أو موجود على الشاشة، تمام؟
+
+256
+00:18:59,560 --> 00:19:04,400
+إذا هذا الـ push، هذا البلانجر عبارة عن كبسة فيها two
+
+257
+00:19:04,400 --> 00:19:07,680
+stops يعني أنا لما أضغط أول ضغطة بلاحظ أنه خلاص
+
+258
+00:19:07,680 --> 00:19:12,780
+وصل إصبعي لدرجة معينة وأنا بضغط، لو ضغطت بقوة أكبر
+
+259
+00:19:12,780 --> 00:19:16,910
+ممكن أضغط الضغطة الثانية، يعني بنزل كمان مرة، فالضغطة
+
+260
+00:19:16,910 --> 00:19:21,410
+الأولى لما أنا بكون ضاغطة إياها بتكون الـ pipet تسحب لي
+
+261
+00:19:21,410 --> 00:19:25,750
+volume نفس الـ volume اللي موجودة على الشاشة، لكن لو
+
+262
+00:19:25,750 --> 00:19:29,690
+أنا بدي أسحب two volumes أو يعني بصراحة بدي أسحب و
+
+263
+00:19:29,690 --> 00:19:33,110
+أنا ضغطتين، هيسحب لي الـ volume اللي موجودة على
+
+264
+00:19:33,110 --> 00:19:37,610
+الشاشة وكمان extra volume ثاني، يعني volume زيادة
+
+265
+00:19:37,610 --> 00:19:40,710
+طب أنا ليش، إمتى بستخدم الضغطة الأولى وإمتى بستخدم
+
+266
+00:19:40,710 --> 00:19:45,100
+الضغطة الثانية أو الضغطتين، هلأ بنشوف، كده هي هنا
+
+267
+00:19:45,100 --> 00:19:47,920
+بيحكي لي أن البلانجر أو اللي هو الـ push button
+
+268
+00:19:47,920 --> 00:19:53,140
+تمام، والـ stop at two different positions يعني هذا
+
+269
+00:19:53,140 --> 00:19:57,740
+البلانجر بيتوقف عند .. عند مكانين، وأنا لما آجي
+
+270
+00:19:57,740 --> 00:20:00,820
+أضغط عليه بلاحظ أنه في عندي two resistance في
+
+271
+00:20:00,820 --> 00:20:05,020
+مقاومتين للضغطة تبعي، الـ .. الـ stop الأول أو
+
+272
+00:20:05,020 --> 00:20:10,160
+الضغطة الأولى، تمام، ولو أنا زودت الـ .. الضغط تبعي
+
+273
+00:20:10,160 --> 00:20:16,630
+بنزل كمان ضغطة ثانية، فبالتالي الـ .. الـ .. الـ first
+
+274
+00:20:16,630 --> 00:20:21,870
+stopping تمام، عند الـ initial resistance تمام، عند
+
+275
+00:20:21,870 --> 00:20:27,610
+أول يعني stop عند أول إعاقة لضغطة تبعي، بيقف الـ ..
+
+276
+00:20:27,610 --> 00:20:30,850
+الـ plunger عندها بتكون هذه عبارة عن الضغطة الأولى
+
+277
+00:20:31,230 --> 00:20:37,590
+طبعاً، و الـ .. و هذه معناها أني أنا هسحب إيش، الـ
+
+278
+00:20:37,590 --> 00:20:40,810
+desired volume يعني أنا هأخذ الـ volume اللي موجود
+
+279
+00:20:40,810 --> 00:20:47,720
+على الشاشة exactly بأخذه، طبعاً الـ second stopping لما
+
+280
+00:20:47,720 --> 00:20:53,380
+يكون .. لما أنا بدي أضغط على الضغط الثاني، يعني أنا
+
+281
+00:20:53,380 --> 00:20:58,900
+بدي أظل نازلة في البلانجر ضغطتين، فساعتها أنا بكون
+
+282
+00:20:58,900 --> 00:21:02,540
+تجاوزت الـ initial resistance عند .. تجاوزت الإعاقة
+
+283
+00:21:02,540 --> 00:21:06,540
+الأولى، وإنزلت كمان مرة في الـ .. في البلانجر، طبعاً
+
+284
+00:21:06,540 --> 00:21:12,600
+فهذا الـ .. الشيء معناها أن الـ pipet راح تسحب لي
+
+285
+00:21:12,600 --> 00:21:17,940
+volume extra طبعاً، هتسحب لي الـ .. الـ volume extra
+
+286
+00:21:17,940 --> 00:21:22,720
+ساعتها أنا لما بدي أفرغ الحجم بأفرغه بضغطة واحدة
+
+287
+00:21:22,950 --> 00:21:26,350
+هينقل الحجم، وهيضل في عندي extra volume موجود في
+
+288
+00:21:26,350 --> 00:21:31,130
+الـ tip ممكن أتخلص منه، تمام؟ إذا في عندي الـ .. الـ
+
+289
+00:21:31,130 --> 00:21:34,370
+.. بلانجر الـ .. أو الـ .. الـ push button بيقف عند
+
+290
+00:21:34,370 --> 00:21:38,430
+ضغطتين، الضغطة الأولى بتنـ .. لما أنا أكون ضاغطة إياها و
+
+291
+00:21:38,430 --> 00:21:42,270
+أسحب حجم من السائل، معناها أنا سحبت نفس الحجم اللي
+
+292
+00:21:42,270 --> 00:21:45,600
+موجودة على الشاشة، لكن إذا أنا كنت ضغطتين وسحبت حجم، معناها أنا سحبت الحجم مع extra تمام، لما
+
+293
+00:21:45,600 --> 00:21:50,120
+آجي أفرغه بدي أإنتبه أني أضغط ضغطة واحدة فقط
+
+294
+00:21:50,120 --> 00:21:54,040
+أفرغ الـ volume اللي من ضغطة واحدة، لأن ضغطة واحدة
+
+295
+00:21:57,520 --> 00:22:00,380
+يعني إيش الـ desired volume يعني الـ volume اللي
+
+296
+00:22:00,380 --> 00:22:04,580
+بدي إياها، وبيضل في عندي extra طبعاً، طب ليش الغلبة هذه
+
+297
+00:22:04,580 --> 00:22:07,880
+ليش ضغطة واحدة وضغطتين، وليش أنا آخذ extra volume
+
+298
+00:22:08,030 --> 00:22:11,550
+عشان في عندي نوعين من الـ pipetting نوع اسمه
+
+299
+00:22:11,550 --> 00:22:16,450
+forward pipetting و reverse pipetting إيش يعني
+
+300
+00:22:16,450 --> 00:22:19,770
+forward pipetting يعني أني أنا أدخل في المحلول
+
+301
+00:22:19,770 --> 00:22:23,870
+تمام، وأنا بضغطة واحدة آخذ الحجم اللي بدي إياه، هو
+
+302
+00:22:23,870 --> 00:22:27,390
+نفس الحجم اللي موجود على الشاشة exactly بالضبط
+
+303
+00:22:27,390 --> 00:22:32,530
+بدون زيادة، وبعد هيك آجي أفرغه، أفرغه في المحلول، في
+
+304
+00:22:32,530 --> 00:22:37,350
+الوعاء اللي أنا بدي أشتغل فيه، وبفرغه، وبضل ضغطة
+
+305
+00:22:37,540 --> 00:22:40,020
+ممكن أضغط أكثر من مرة أو ممكن أضغط أكثر من مرة أو
+
+306
+00:22:40,020 --> 00:22:44,880
+ممكن أضغط أكثر من مرة أو ممكن أضغط أكثر من مرة أو
+
+307
+00:22:44,880 --> 00:22:48,820
+ممكن أضغط أكثر من مرة أو ممكن أضغط أكثر من مرة أو
+
+308
+00:22:48,820 --> 00:22:48,960
+ممكن أضغط أكثر من مرة أو ممكن أضغط أكثر من مرة أو
+
+309
+00:22:48,960 --> 00:22:49,180
+ممكن أضغط أكثر من مرة أو ممكن أضغط أكثر من مرة أو
+
+310
+00:22:49,180 --> 00:22:49,860
+ممكن أضغط أكثر من مرة أو ممكن أضغط أكثر من مرة أو
+
+311
+00:22:49,860 --> 00:22:52,000
+ممكن أضغط أكثر من مرة أو ممكن أضغط أكثر من مرة أو
+
+312
+00:22:52,000 --> 00:22:53,780
+ممكن أضغط أكثر من مرة، إذا إيش الـ forward
+
+313
+00:22:53,780 --> 00:22:57,380
+pipetting؟ معناها .. معناها أني أنا أضغط ضغطة على الـ
+
+314
+00:22:57,380 --> 00:23:01,680
+pipet بعدين آجي أسـ .. أـ .. أنقل الحجم، آخذ الحجم
+
+315
+00:23:01,680 --> 00:23:04,200
+اللي بدي إياه، هو الـ exact volume اللي موجود على
+
+316
+00:23:04,200 --> 00:23:08,120
+الشاشة، ولما آجي أفرغه في الوعاء ممكن أضغط ضغط أو
+
+317
+00:23:08,120 --> 00:23:11,040
+ضغطتين عشان أتأكد إني أنا فرغته كله، وإني
+
+318
+00:23:11,040 --> 00:23:14,700
+استخدمت الـ volume بالضبط، هو نفس اللي موجود على
+
+319
+00:23:14,700 --> 00:23:17,720
+الشاشة، تمام؟ ما يكونش في ضاـ .. إيش، يضل عالق في الـ
+
+320
+00:23:17,720 --> 00:23:23,070
+tip تمام؟ إذا .. هذا معناه الـ forward وهذه الطريقة
+
+321
+00:23:23,070 --> 00:23:27,410
+تستخدم عشان أنا لو بدي أنقل حجوم زي مين زي الـ
+
+322
+00:23:27,410 --> 00:23:31,450
+aqueous solutions المحاليل اللي أصلها ماء، الـ buffers
+
+323
+00:23:31,450 --> 00:23:35,310
+الـ acids، الـ bases، كل الأشياء المحاليل هذه اللي أصلها
+
+324
+00:23:35,310 --> 00:23:39,750
+ماء، بستخدم معاها طريقة الـ forward pipetting أما
+
+325
+00:23:39,750 --> 00:23:43,390
+طريقة الـ reverse pipetting فهي عبارة عن أني أنا
+
+326
+00:23:43,390 --> 00:23:47,990
+بأخذ larger volume of solution بأصحب larger volume
+
+327
+00:23:47,990 --> 00:23:51,990
+of solution إيش يعني؟ يعني بأصحب extra يعني معناها
+
+328
+00:23:51,990 --> 00:23:55,430
+أنا لما باجي بأخذ الـ pipet أو بنقل في الـ pipet
+
+329
+00:23:55,430 --> 00:24:00,870
+بكون ضغطة، ضغطتين، تمام؟ بأخذ الحجم اللي بدي إياه مع
+
+330
+00:24:00,870 --> 00:24:05,750
+extra تمام؟ ولما آجي أفرغ في المحلول بأفرغ بواحدة
+
+331
+00:24:05,750 --> 00:24:11,030
+بحيث إني أنا أفرغ فقط المحلول، الحجم اللي بدي إياه، ويضل
+
+332
+00:24:11,030 --> 00:24:14,330
+الـ extra اللي موجود في الـ tip أتخلص منه، طب أنا ليش
+
+333
+00:24:14,330 --> 00:24:20,430
+يعني بلجأ لهذه الطريقة؟ بيحكي لي لإني في سوائل عبارة
+
+334
+00:24:20,430 --> 00:24:25,250
+عن لزجة viscous طبعاً، هذا إيش يعني viscous؟ لزجة
+
+335
+00:24:25,250 --> 00:24:29,130
+يعني بصعوبة هتطلع جوة الـ tip، المحلول بصعوبة هتطلع
+
+336
+00:24:29,130 --> 00:24:35,520
+جوة الـ tip طبعاً، طيب، فبالتالي لما آجي أنا أكون أخذة
+
+337
+00:24:35,520 --> 00:24:39,200
+الـ volume وزيادة شوية، هكون ضامنة إني أنا أخدت الـ
+
+338
+00:24:39,200 --> 00:24:42,060
+volume .. يعني ضامنة إني أنا هنزل الـ volume على
+
+339
+00:24:42,060 --> 00:24:47,480
+الأقل اللي بدي إياه، تمام؟ لكن مثلاً لو أنا ده استخدمت
+
+340
+00:24:47,480 --> 00:24:52,430
+مع هذه المحاليل اللزجة viscous استخدمت نظام ضغطة واحدة
+
+341
+00:24:52,430 --> 00:24:57,050
+ما هي بتطلع جوة الـ tip ببطء، يعني أنا ممكن أدخل وأطلع
+
+342
+00:24:57,050 --> 00:25:01,590
+وأخذ الـ باقي الحجم اللي بدي إياه، بس لسه السائل من
+
+343
+00:25:01,590 --> 00:25:08,200
+كتر لزوجته ما بيطلع جوة الـ tip بشكل كامل، فبالتالي ممكن
+
+344
+00:25:08,200 --> 00:25:12,800
+آخذ volume أقل من اللي أنا بدي إياه، فهنا ممكن يكون في
+
+345
+00:25:12,800 --> 00:25:17,120
+خطأ، عشان هيك بيحكي لي أنه استخدمي مع السوائل اللزجة
+
+346
+00:25:17,120 --> 00:25:21,200
+أنه طريقة أنك أنت تأخذي بزيادة، ولما تفرغي تفرغي
+
+347
+00:25:21,200 --> 00:25:27,230
+الحجم اللي بدك إياه، تمام؟ هذا بالنسبة لمين؟ لـ .. الـ ..
+
+348
+00:25:27,230 --> 00:25:31,150
+الـ .. السوائل اللزجة، بستخدم معاها الـ reverse
+
+349
+00:25:31,150 --> 00:25:34,330
+technique يعني نظام الضغطين عشان آخذ extra volume
+
+350
+00:25:34,330 --> 00:25:37,590
+أيضاً بيحكي لي فيه محاليل volatile إيش يعني
+
+351
+00:25:37,590 --> 00:25:42,030
+volatile؟ يعني متطايرة، يعني مجرد مثلاً ما أسحبها في
+
+352
+00:25:42,030 --> 00:25:46,350
+الـ pipet وأنقلها للـ .. للوعاء عشان أفرغها ممكن
+
+353
+00:25:46,350 --> 00:25:50,810
+تكون اتبخرت، فهنا بيصير في عندي فاقد في الحجم
+
+354
+00:25:50,810 --> 00:25:58,290
+فبالتالي أنا بكون نقلت حجم أقل من اللازم، فهنا بأستعمل
+
+355
+00:25:58,290 --> 00:26:05,490
+هذه النوع من السوائل، أيضاً ممكن أني أستخدم نظام
+
+356
+00:26:05,490 --> 00:26:09,230
+الضغطين، بحيث إني أنا آخذ حجم بزيادة، ولما أفرغ أفرغ
+
+357
+00:26:09,230 --> 00:26:14,030
+exact volume عشان مهما يكون الطاير من هذا الـ
+
+358
+00:26:14,030 --> 00:26:18,050
+solution أكون على الأقل مازالت محتفظة بالـ volume
+
+359
+00:26:18,050 --> 00:26:21,890
+اللي أنا بدي إياه، تمام؟ أيضاً في بعض المحاليل اللي
+
+360
+00:26:21,890 --> 00:26:24,510
+بنسميها foaming solution إيش يعني foaming
+
+361
+00:26:24,510 --> 00:26:28,950
+solution؟ يعني محاليل بتعمل رغوة، تمام؟ فيها مادة
+
+362
+00:26:28,950 --> 00:26:33,190
+صابون أو يعني مادة .. لها ملمس الصابونة، هذه
+
+363
+00:26:33,190 --> 00:26:37,690
+المحاليل اللي فيها مواد ذات ملمس الصابونة، تمام؟
+
+364
+00:26:37,690 --> 00:26:41,120
+ممكن تعمل رغوة، وبالتالي لما آجي أسحب أسحب من
+
+365
+00:26:41,120 --> 00:26:45,220
+الرغوة، high فبالتالي أكون نقلت volume أقل من
+
+366
+00:26:45,220 --> 00:26:49,520
+اللازم، لإني أنا يعني جزء من الرغوة احتل الـ tip
+
+367
+00:26:49,520 --> 00:26:53,300
+تمام؟ وأظن حالي إني أنا أخذت الحجم اللي بدي إياه، و
+
+368
+00:26:53,300 --> 00:26:59,700
+فعلياً أنا بكون أخذة حجم أقل من الـ .. المفروض
+
+369
+00:26:59,700 --> 00:27:03,300
+فبالتالي بأستخدم برضه نظام الـ larger volume of
+
+370
+00:27:03,300 --> 00:27:06,120
+solution يعني إني أنا بدي آخذ الـ extra volume يعني
+
+371
+00:27:06,120 --> 00:27:09,700
+بدي أستخدم الـ reverse technique يعني نظام ضغطتين و
+
+372
+00:27:09,700 --> 00:27:17,300
+.. وبعد هيك شسمه بروح بأفرغ بضغطة واحدة، تمام؟ عشان
+
+373
+00:27:17,300 --> 00:27:21,780
+لو كان في رغوة في المحلول أكون ضامنة أنها ما تأثر
+
+374
+00:27:21,780 --> 00:27:25,360
+على الحجم اللي أنا بدي أنقله، تمام؟ وأيضاً عشان لو
+
+375
+00:27:25,360 --> 00:27:29,180
+أنا بدي أنقل حجوم صغيرة جداً جداً، يعني أنا خائفة ..
+
+376
+00:27:29,180 --> 00:27:32,260
+يعني مش متأكدة أخدت الـ volume ولا لأ، لأنه في حجوم
+
+377
+00:27:32,260 --> 00:27:35,600
+يعني زي مثلاً حجم one microliter يعني أنت حتى ما
+
+378
+00:27:35,600 --> 00:27:39,040
+بتشوفيه على الـ tip يعني صعب تشوفيه على الـ tip أنه أنت
+
+379
+00:27:39,040 --> 00:27:42,720
+أخذتي أو ما أخذتي، فالأفضل أني أنا آخذ حجم بزيادة و
+
+380
+00:27:42,720 --> 00:27:45,560
+لما أفرغ داخل الـ solution أو المقلوعه اللي أنا بدي
+
+381
+00:27:45,560 --> 00:27:50,640
+أشتغل فيه بأفرغ بالحجم اللي بدي إياه، إذا .. إذا لما
+
+382
+00:27:50,640 --> 00:27:56,550
+أنا أسحب الحجم بضغطة وأفرغه بضغطتين، معناها أنا بدي
+
+383
+00:27:56,550 --> 00:28:01,050
+آخذ exact volume وأفرغه بضغطتين عشان أضمن أن
+
+384
+00:28:01,050 --> 00:28:05,270
+أنا فرغته كويس، تمام؟ هذه طريقة forward pipetting
+
+385
+00:28:05,270 --> 00:28:10,510
+لكن لما أنا أسحب الحجم بضغطتين وأفرغه بضغطة واحدة
+
+386
+00:28:10,510 --> 00:28:14,590
+معناها أنا استخدمت طريقة الـ reverse pipetting وهذا
+
+387
+00:28:14,590 --> 00:28:20,490
+طبعاً بيختلف، هلأ الـ .. الـ .. إذا هذه الـ pipet شايفين
+
+388
+00:28:20,490 --> 00:28
+
+445
+00:32:24,510 --> 00:32:29,230
+tip مغمور داخل الـ solution و برفع إيدي بالتدريج عن
+
+446
+00:32:29,230 --> 00:32:32,670
+الـ plunger بسحب لي الـ volume، طبعا هنا في الـ reverse
+
+447
+00:32:32,670 --> 00:32:37,190
+أنا ضغطت ضغطتين فهيسحب لي extra volume، تمام؟ بعدين
+
+448
+00:32:37,190 --> 00:32:40,570
+بروْح على الـ container اللي أنا بدي أفْضِي فيه و بضغط
+
+449
+00:32:40,570 --> 00:32:44,230
+ضغطة واحدة علشان تنزلي الـ solution اللي أنا بديها
+
+450
+00:32:44,230 --> 00:32:48,210
+فقط الحجم اللي بديها فقط، و الـ extra هو و الـ tab
+
+451
+00:32:48,210 --> 00:32:50,730
+بتخلص منه في الـ waste من خلال أن أنا أضغط على الـ
+
+452
+00:32:50,730 --> 00:32:54,590
+ejector و تنفصل الـ tab عن الـ pipet و تروح في حال
+
+453
+00:32:54,590 --> 00:33:00,370
+سبيلها، تمام؟ أول حاجة مع .. يعني ملاحظات لاستخدام
+
+454
+00:33:00,370 --> 00:33:04,650
+الـ pipet، never point a pipet up، ممنوع أنك أنتِ
+
+455
+00:33:04,650 --> 00:33:07,970
+تستخدمي الـ pipet تقليبيها بهذا الشكل بحيث أن
+
+456
+00:33:07,970 --> 00:33:13,600
+تكون الـ tip صارت فوق و الـ pipet تحت، طبعا و أنا
+
+457
+00:33:13,600 --> 00:33:16,300
+دايما لما بستخدم الـ pipet بتكون بهذا الاتجاه بحيث
+
+458
+00:33:16,300 --> 00:33:19,620
+أنه الـ tip لا تحت، لإنه لو أنا بدي أجلب الـ volume
+
+459
+00:33:19,620 --> 00:33:22,680
+اللي موجود في الـ tip راح يفوت داخل الـ pipet، طبعا هذه
+
+460
+00:33:22,680 --> 00:33:28,420
+عبارة عن pipet طبعا، و في كمان نظام الـ ... الـ ...
+
+461
+00:33:28,420 --> 00:33:33,460
+الـ  ذَنَبِرْكات، فبالتالي ممكن تصدأ داخل الـ ... يعني يصير
+
+462
+00:33:33,460 --> 00:33:37,300
+صدأ أو يصير فيه تلف، و ممكن في محاليل بترسب بتعمل
+
+463
+00:33:37,300 --> 00:33:41,440
+رواسب بتعمل انسداد، فهذا بيأثر على دقة الـ pipet، فهو
+
+464
+00:33:41,440 --> 00:33:45,340
+يفضل أن أنا ممنوع أوجهها إلى أعلى بحيث أن الـ
+
+465
+00:33:45,340 --> 00:33:48,580
+liquid يفوت داخل الـ pipet و يعملها destroying
+
+466
+00:33:48,580 --> 00:33:53,060
+تمام؟ طيب when withdrawing liquid with a
+
+467
+00:33:53,060 --> 00:33:56,540
+pipet، it always release the plunger slowly، طبعا
+
+468
+00:33:56,540 --> 00:34:00,720
+لحظة لاحظتوا أنتوَ حتى بنبرة صوتي، ركزتوا كتير منيح و
+
+469
+00:34:00,720 --> 00:34:04,020
+أنا بحكي لكم لما ترفعوا إيدكم عن الـ plunger ارفعوها
+
+470
+00:34:04,020 --> 00:34:09,040
+slowly، شوية شوية بالتدريج بهدوء، ما برفع إيدي فجأة
+
+471
+00:34:09,040 --> 00:34:13,800
+ليش؟ لإنه هنا هي بتحكي لك أنه الـ ... الـ ... الـ ... الشغل
+
+472
+00:34:13,800 --> 00:34:19,280
+رافع إيدك بشكل تدريجي أو بشكل بطيء عن الـ plunger
+
+473
+00:34:19,280 --> 00:34:24,080
+هَيمنع من أن الـ liquid يصير له rushing into، أنه
+
+474
+00:34:24,080 --> 00:34:28,160
+يندفع داخل الـ pipet و بالتالي ممكن يسبب لها انسداد
+
+475
+00:34:28,160 --> 00:34:35,100
+أو صدأ أو تلف، فبالتالي مهم جدا طبعا أن ترفعوا إيدكم
+
+476
+00:34:35,100 --> 00:34:38,020
+بالتدريج، this is especially important with large
+
+477
+00:34:38,020 --> 00:34:41,860
+volumes، يعني و هذا الشيء بنتبه له كتير منيح و أنا
+
+478
+00:34:41,860 --> 00:34:46,740
+بأسحب أحجام كبيرة، أحجام كبيرة معناها السائل هيندفع
+
+479
+00:34:46,740 --> 00:34:50,720
+بشكل قوي، هو كميته كبيرة فهيفوت داخل الـ pipet هيصل لـ
+
+480
+00:34:50,720 --> 00:34:55,240
+الـ pipet من جوا و يتلفها، be sure you use the proper
+
+481
+00:34:55,240 --> 00:35:02,780
+size tip for each pipet، حكينا أنه لكل pipet طبعا لكل
+
+482
+00:35:02,780 --> 00:35:08,040
+pipet في عندي tip خاصة فيها تبعا لحجمها أو تبعا لـ الـ
+
+483
+00:35:08,040 --> 00:35:12,380
+range تبعها، فأنا كل pipet بستخدم لها الـ tip الخاصة
+
+484
+00:35:12,380 --> 00:35:15,340
+فيها، ما بنفع مثلا مع الـ pipettes الصغيرة أستخدم tip
+
+485
+00:35:15,340 --> 00:35:19,040
+كبيرة، أصلا مش راح تركب عليها، و ما بنفع مع الـ pipettes
+
+486
+00:35:19,040 --> 00:35:21,980
+الكبيرة أستخدم tip صغيرة، مش راح تكفي للـ volume اللي
+
+487
+00:35:21,980 --> 00:35:26,520
+أنا بدي أنقله طبعا، فإذن كل pipet لها tip خاصة فيها
+
+488
+00:35:26,520 --> 00:35:30,740
+بستخدمها، always use a new tip for each different
+
+489
+00:35:30,740 --> 00:35:35,960
+liquid، طبعا كل حجم بدي أنقله بستخدم له a new tip
+
+490
+00:35:35,960 --> 00:35:40,020
+طبعا الـ tip جديدة، و حكينا أن هي الـ tip أسمها
+
+491
+00:35:40,020 --> 00:35:44,460
+disposable، طبعا فهيمر معنا إن شاء الله مصطلح
+
+492
+00:35:44,460 --> 00:35:48,360
+disposable و أذكركم فيه، يعني تستخدم لمرة واحدة فقط
+
+493
+00:35:48,640 --> 00:35:52,580
+طيب، use the correct pipet for the volume to be
+
+494
+00:35:52,580 --> 00:35:56,260
+dispensed، يعني أنا بدي أستخدم الـ pipet، أختار الـ
+
+495
+00:35:56,260 --> 00:36:02,180
+pipet اللي الـ range تبعها تمام مناسب لـ ... الـ ... الـ
+
+496
+00:36:02,180 --> 00:36:04,980
+... الـ pipet للحجم اللي بدي أنقله، هي مثلا هنا
+
+497
+00:36:04,980 --> 00:36:09,420
+بيحكي لك never use the  متين لـ ألف microliter pipet
+
+498
+00:36:09,420 --> 00:36:12,720
+to dispense متين، تمام؟ هي هنا مثلا هي الـ range من
+
+499
+00:36:12,720 --> 00:36:18,210
+متين لـ ألف، ما بستخدمها عشان أنقل حجم متين، لأن حكينا
+
+500
+00:36:18,210 --> 00:36:20,870
+أنها مش هتكون دقيقة على lower limit لكن ممكن تكون
+
+501
+00:36:20,870 --> 00:36:23,630
+دقيقة على upper limit أو بتكون أدق على upper
+
+502
+00:36:23,630 --> 00:36:28,750
+limit، تمام؟ و لو أنا بدي آخذ أحجام أقل من 200 أو
+
+503
+00:36:28,750 --> 00:36:33,790
+أكثر من ألف، هذا إيش ممكن يعملي؟ يسبب لي damage للأداة
+
+504
+00:36:33,790 --> 00:36:39,710
+هذه، فبالتالي لازم أكون حذرة و حريصة في استخدام الـ
+
+505
+00:36:39,710 --> 00:36:44,850
+pipet، without the pipet، withdraw the pipet from the
+
+506
+00:36:44,850 --> 00:36:48,290
+receiving vessels carefully، sliding the tip along
+
+507
+00:36:48,290 --> 00:36:51,890
+the wall، هلأ مجرد ما أنا أرفع الـ pipet بعد ما آخذ
+
+508
+00:36:51,890 --> 00:36:55,610
+الحجم اللي بدي إياه لازم أعمل حاجة اسمها sliding يعني
+
+509
+00:36:55,610 --> 00:37:00,750
+أنا أَمرِّر الـ tip هادي، أمسحها على فتحة الـ container
+
+510
+00:37:00,750 --> 00:37:05,690
+يعني مثلا بهذا الشكل، يعني أرفع و أنزل الـ pipet على
+
+511
+00:37:05,690 --> 00:37:11,130
+الـ فوهة أو فتحة الـ container تبع المحلول بحيث
+
+512
+00:37:11,130 --> 00:37:15,910
+أنه أي شيء عالق على جدار الـ tip من برا أخلص منه
+
+513
+00:37:15,910 --> 00:37:19,330
+أنا بعنيني فقط الـ volume اللي أنا نقلته في الداخل
+
+514
+00:37:19,330 --> 00:37:23,670
+طبعا، فبالتالي عشان ما آخذ excess volume، remove the
+
+515
+00:37:23,670 --> 00:37:30,770
+tip from the sample liquid، تمام؟ و no liquid should
+
+516
+00:37:30,770 --> 00:37:35,130
+remain on the outside of the tip، طبعا يعني لما ...
+
+517
+00:37:35,130 --> 00:37:40,130
+مجرد ما أنا أطلع من الـ ... الـ ... يعني آخذ الحجم
+
+518
+00:37:40,130 --> 00:37:43,410
+اللي بدي إياه و أطلع من الـ container، مش لازم يضل
+
+519
+00:37:43,410 --> 00:37:47,910
+بقايا من الـ solution على الـ tip، يفضل أن أنا أمسح
+
+520
+00:37:47,910 --> 00:37:52,620
+باستخدام الـ wipe، تمام؟ أو الـ ... الـ gauze أو أي قطعة
+
+521
+00:37:52,620 --> 00:37:58,780
+... قطعة قطن أو شاش، بأجفف الـ ... الـ
+
+522
+00:37:58,780 --> 00:38:02,780
+... الـ pipet، بمسحها، بمررها عليها بحيث أن أنا أجفف
+
+523
+00:38:02,780 --> 00:38:07,300
+أي بقايا عالقة عليها، ولكن أكون حريصة أنه
+
+524
+00:38:07,300 --> 00:38:12,600
+هادي الـ ... الـ ... الـ pipet تمام، ما تلامس فوهة الـ tip
+
+525
+00:38:12,600 --> 00:38:17,540
+ليش؟ لإنه لو لامست فوهة الـ tip، هذا absorbent، المحارم
+
+526
+00:38:17,540 --> 00:38:21,000
+و الـ gauze و الشيء بينطس السائل، فممكن يمتص
+
+527
+00:38:21,000 --> 00:38:25,820
+السائل اللي موجود داخل الـ tip و بالتالي بينقص الـ
+
+528
+00:38:25,820 --> 00:38:28,560
+volume اللي أخذته، و بيكون أنا هيك عم بأخذ volumes
+
+529
+00:38:28,560 --> 00:38:32,260
+مش accurate و مش precise، تمام؟ مهم جدا هذه الـ ...
+
+530
+00:38:32,260 --> 00:38:37,440
+الشغلة، إذا بعد هي مجرد ما إنه أسحب الـ ... الـ ... الـ
+
+531
+00:38:37,440 --> 00:38:37,860
+... الـ ... الـ ... الـ ... الـ ... الـ ... الـ ... الـ
+
+532
+00:38:37,860 --> 00:38:38,800
+الـ ... الـ ... الـ ... الـ ... الـ ... الـ ... الـ ... الـ
+
+533
+00:38:38,800 --> 00:38:39,540
+... الـ ... الـ ... الـ ... الـ ... الـ ... الـ ... الـ ...
+
+534
+00:38:39,540 --> 00:38:39,680
+الـ ... الـ ... الـ ... الـ ... الـ ... الـ ... الـ ... الـ
+
+535
+00:38:39,680 --> 00:38:43,650
+الـ ...  مش لازم no liquid should remain on the
+
+536
+00:38:43,650 --> 00:38:46,950
+outside، مش لازم الـ liquid يضل على خارج الـ tip
+
+537
+00:38:46,950 --> 00:38:51,470
+فبالتالي بمسح بقايا اللي ... اللي موجودة على الـ
+
+538
+00:38:51,470 --> 00:38:56,190
+outside of the tip باستخدام tissue، paper، gauze،
+
+539
+00:38:56,730 --> 00:39:01,130
+wipes، أي شيء ... أي نوع محارم أو gauze بحيث أنه ما
+
+540
+00:39:01,130 --> 00:39:04,790
+ألامس، never touch the tip orifice، ما ألامسش
+
+541
+00:39:04,910 --> 00:39:08,290
+الـ tip orifice، يعني فتحة الـ ... الـ tip، الـ opening
+
+542
+00:39:08,290 --> 00:39:12,410
+تبع الـ tip، طبعا ما ألامسه بحيث أنه ما ينطس السائل و
+
+543
+00:39:12,410 --> 00:39:16,770
+بهيك أنا أكون عم بأخذ volume أقل من اللازم و أكون
+
+544
+00:39:16,770 --> 00:39:19,870
+مش ... مش accurate و مش precise في عملية الـ volume
+
+545
+00:39:19,870 --> 00:39:25,090
+transfer، طبعا، طيب pre-rinsing، إيش يعني كلمة pre
+
+546
+00:39:25,090 --> 00:39:31,610
+-rinsing؟ يفضل أن أنا ... أنا لما بدي أنقل أحجام أن
+
+547
+00:39:31,610 --> 00:39:39,390
+أنا أبلل، يعني مثلا هنا يفضل إن أنا أبلل الـ ... الـ ...
+
+548
+00:39:39,390 --> 00:39:44,290
+الـ tip من جوا في ... في ... في السائل اللي بدي أنقله،
+
+549
+00:39:44,290 --> 00:39:47,510
+هاي معناها pre-rinsing، طبعا؟ يعني أول حاجة بأسحب أول
+
+550
+00:39:47,510 --> 00:39:52,330
+volume و بتخلص منه بكبه، هيك أنا بضمن أنه جزء من
+
+551
+00:39:52,330 --> 00:39:56,570
+السائل لمس الـ ... الـ ... الـ tip من الداخل فبالتالي
+
+552
+00:39:56,570 --> 00:40:02,570
+صارت الـ tip أسمها مبللة، طيب، فلمّا أجي أنقل volume
+
+553
+00:40:03,230 --> 00:40:06,390
+و آخذ الـ volume و أنقله، هيك بكون أنا عم بأخذ
+
+554
+00:40:06,390 --> 00:40:10,490
+volume بشكل أدق، ليش؟ لأنه لو ... لو أنا ما عملت الـ
+
+555
+00:40:10,490 --> 00:40:14,490
+pre-rinsing هذه، طبعا لو ما عملت pre-rinsing، أخدت
+
+556
+00:40:14,490 --> 00:40:18,350
+volume و رحت فضيته، جزء من السائل اللي أنا
+
+557
+00:40:18,350 --> 00:40:24,370
+أخذته بيضل ملتصق بجدران الـ tip مبلل لها، وهذا جزء
+
+558
+00:40:24,370 --> 00:40:29,190
+يعني في نسبة فقد للـ volume، في نسبة error، طبعا لكن
+
+559
+00:40:29,190 --> 00:40:34,490
+لما أنا أستخدم tip already مبللة، يعني tip أنا سحبت
+
+560
+00:40:34,490 --> 00:40:36,970
+أول volume فيها و كبيته، و بعدين سحبت الـ volume
+
+561
+00:40:36,970 --> 00:40:40,290
+اللي بدي أستخدمه، الـ volume الفعلي، معناها أنا بللت
+
+562
+00:40:40,290 --> 00:40:45,850
+الـ tip من الداخل، فبالتالي فبطل في عندي نسبة فقد من
+
+563
+00:40:45,850 --> 00:40:49,890
+الحجم و بطل في عندي نسبة الـ error، هذه معناها كلمة
+
+564
+00:40:49,890 --> 00:40:53,670
+pre-rinsing، minimize أسمها الـ variances،
+
+565
+00:40:53,670 --> 00:40:59,560
+الاختلافات في الـ ... الـ ... الأحجام، طبعا when by
+
+566
+00:40:59,560 --> 00:41:03,080
+pipetting some solutions زي الـ serum اللي هي العينات
+
+567
+00:41:03,080 --> 00:41:08,260
+يعني الشخْص ... المرضى، الـ serum، البلازما، الـ protein
+
+568
+00:41:08,260 --> 00:41:11,300
+containing solution، المحاليل اللي بتحتوي على
+
+569
+00:41:11,300 --> 00:41:14,420
+البروتينات، الـ organic solvents، المذيبات العضوية،
+
+570
+00:41:14,420 --> 00:41:19,700
+هذه طبعا في جزء منها بيكون حكينا أنه هيصير في فيلم،
+
+571
+00:41:19,700 --> 00:41:24,500
+فيه جزء منها هيبقى هيلمس الـ inside of the tip و
+
+572
+00:41:24,500 --> 00:41:29,200
+هذا حكينا أسمه error، فبالتالي لما أنا أعمل عملية
+
+573
+00:41:29,200 --> 00:41:33,540
+pre-rinsing، فبالتالي أنا بقلل الـ error هذه من خلال
+
+574
+00:41:33,540 --> 00:41:39,120
+أن أنا خلاص استخدمت already جزء بيْبَة مبللة، جزء ...
+
+575
+00:41:39,120 --> 00:41:43,040
+بيبة عملنا لها pre-rinsing، فبالتالي مش هيكون في
+
+576
+00:41:43,040 --> 00:41:47,400
+عندي فقد في الـ volume و مش هيكون في عندي نسبة
+
+577
+00:41:47,400 --> 00:41:51,780
+error، طيب when by pipetting concentrated acid or
+
+578
+00:41:51,780 --> 00:41:56,100
+highly corrosive solution such as halide salts،
+
+579
+00:41:56,100 --> 00:42:01,540
+it's recommended that the pipet be disassembled
+
+580
+00:42:01,540 --> 00:42:07,680
+after used to inspect the shaft و المكونات تبعها،
+
+581
+00:42:07,680 --> 00:42:11,800
+هلأ بيحكي لك أنه لما بنستخدم بعض المحاليل اللي هي
+
+582
+00:42:11,800 --> 00:42:18,100
+corrosive، تمام؟ حارقة أو يعني مؤذية و بيطير منها
+
+583
+00:42:18,100 --> 00:42:24,160
+أبخرة أو يعني فيه بيصعد منها قُطَرانَات أو أبخرة أو
+
+584
+00:42:24,160 --> 00:42:28,960
+رذاذ زي الـ ... الـ ... halide salts، هاي يفضل أن أنا
+
+585
+00:42:28,960 --> 00:42:32,620
+أفك الـ pipet و أنظفها من الداخل و أرجع أركبها ثانية
+
+586
+00:42:32,620 --> 00:42:38,940
+علشان أتأكد إنها هي يعني ما رح تتعطل، ما رح تخرب، الـ
+
+587
+00:42:38,940 --> 00:42:44,050
+air bubble، طبعا يعني أن في عندي تقطيع في السائل اللي
+
+588
+00:42:44,050 --> 00:42:47,170
+أنا عم بأخذه، في هنا فيه قطرات، فيه ... فيه هواء،
+
+589
+00:42:47,170 --> 00:42:52,670
+تمام؟ طيب هذا الـ ... الـ ... الهواء إيش سببه؟ سببه أول
+
+590
+00:42:52,670 --> 00:42:55,970
+حاجة إما إن أنا رفعت إيدي عن الـ plunger بشكل سريع
+
+591
+00:42:55,970 --> 00:43:00,010
+أو كانت الـ tip مش مغمورة كويس في الـ solution، سحبت
+
+592
+00:43:00,010 --> 00:43:03,550
+من عن السطح، فبالتالي بما إن سحبت من السطح معناها
+
+593
+00:43:03,550 --> 00:43:10,810
+صار في عرض لإنه يدخل air bubble، تمام؟ ممكن يكون إنه
+
+594
+00:43:11,460 --> 00:43:19,940
+سببها الـ ... الـ ... يعني السائل نفسه يكون يعني فومي،
+
+595
+00:43:19,940 --> 00:43:26,480
+بي ... بيكون رغوة، فبالتالي أنا ما أتبعت التعليمات لـ
+
+596
+00:43:26,480 --> 00:43:31,360
+... لـ ... لـ ... الـ pipetting من فومي reagent،
+
+597
+00:43:31,360 --> 00:43:36,240
+فبالتالي ممكن تظهر عندي air bubble، طبعا الـ ... الـ
+
+598
+00:43:36,240 --> 00:43:40,190
+... الـ air bubble إذا ظهرت، طبعا، طبعا أول حاجة إذا
+
+599
+00:43:40,190 --> 00:43:43,910
+ظهرت في ... في الـ pipet، بأبدل solution و برجع بأخذ
+
+600
+00:43:43,910 --> 00:43:47,490
+مرة ثانية، ضلت موجودة، معناها بِبدَّل أخلص من الـ tip و
+
+601
+00:43:47,490 --> 00:43:51,090
+أركب tip جديدة و آخذ volume مع الـ ... الالتزام
+
+602
+00:43:51,090 --> 00:43:56,410
+بالتعليمات حتى أتجنب من ظهور air bubble، ليش؟ لإنه
+
+603
+00:43:56,410 --> 00:43:59,150
+الـ air bubble طبعا هذه محتلة جزء من الحجم اللي
+
+604
+00:43:59,150 --> 00:44:03,580
+موجود، يعني هذا المفروض كله يكون سائل، طبعا فوجود air
+
+605
+00:44:03,580 --> 00:44:06,860
+bubble، وجود تقطيع في السائل يعني فيه هواء، فبالتالي
+
+606
+00:44:06,860 --> 00:44:09,900
+الهواء محتل حجم، إذا أنا مش ماخدة الحجم المناسب،
+
+607
+00:44:09,900 --> 00:44:14,360
+ماخدة أقل منه، إذا أنا مش accurate و مش precise، طبعا
+
+608
+00:44:14,360 --> 00:44:19,420
+هذا بالنسبة لـ الـ pipet، طيب خلينا بس قبل ما نشوف الـ
+
+609
+00:44:19,420 --> 00:44:23,580
+calibration لـ الـ pipet نتابع مع بعض، نشوف هذا
+
+610
+
+667
+00:48:55,290 --> 00:48:59,850
+تمام هي هنا  الـ volume هي هنا ضغطة، هي ضغطتين
+
+668
+00:48:59,850 --> 00:49:03,690
+تمام، تعلمنا الضغطة والضغطتين، وهي هنا شايفين كده
+
+669
+00:49:03,690 --> 00:49:08,430
+أنا بفوت ضغطة، وبعدين ب... بسحب ال... ب... بترك
+
+670
+00:49:08,430 --> 00:49:12,630
+إيدي شوية شوية عن البلانجر، مابنفع أنا أفوت مش ضغطة
+
+671
+00:49:12,630 --> 00:49:16,250
+وأضغط جوا الـ solution، ليش؟ لإنه ساعتها أنا بكون
+
+672
+00:49:16,250 --> 00:49:18,890
+بدخل هوا جوا الـ solution، إذا هو من النوع الفومي
+
+673
+00:49:18,890 --> 00:49:21,890
+اللي بيكون رغوة، هيصير فيه رغوات ويصير في عندي air
+
+674
+00:49:21,890 --> 00:49:26,950
+bubble ومشاكل في نقل الحجوم، طبعا طيب هلأ أنا بعد
+
+675
+00:49:26,950 --> 00:49:29,730
+ما أنا فضيت الـ volume، ضغطت على البلانجر وفضيت الـ
+
+676
+00:49:29,730 --> 00:49:32,350
+volume، إيش بده أعمل؟ بده أضغط على الـ ejector عشان
+
+677
+00:49:32,350 --> 00:49:36,370
+أتخلص من الـ tip، شايفين مجرد ما أضغط عليها ال... الـ
+
+678
+00:49:36,370 --> 00:49:40,890
+... الـ tip بتفصل عن الـ bibert وبت... بتم التخلص منها
+
+679
+00:49:40,890 --> 00:49:46,460
+طبعا هذا بالنسبة للـ... لل... للـ bybite، إذا أول
+
+680
+00:49:46,460 --> 00:49:52,560
+حاجة بنحدد الحجم، ب... أركب الـ tip، بعمل pre-rinsing
+
+681
+00:49:52,560 --> 00:49:58,200
+ببللها تمام، وبعدين باخد الحجم اللي بدي إياه
+
+682
+00:49:58,200 --> 00:50:01,960
+سواء كان باخده بطريقة ال...ال...الـ forward
+
+683
+00:50:01,960 --> 00:50:06,000
+bybiting أو الـ reverse bybiting حسب اللي أنا الحجم
+
+684
+00:50:06,000 --> 00:50:09,930
+اللي ال...ال...أو السائل اللي أنا بتعامل معه
+
+685
+00:50:09,930 --> 00:50:15,030
+بانقله من وإلى، بعد هيك بتخلص من الـ tip بهذا الشكل
+
+686
+00:50:15,030 --> 00:50:21,550
+تمام؟ إن شاء الله هيكون شغلنا على ال...الـ bybit و
+
+687
+00:50:21,550 --> 00:50:24,630
+طبعا معظم...كل الفحوصات بتنعمل باستخدام الـ bybit
+
+688
+00:50:24,630 --> 00:50:28,630
+أنا بدي أضيف حجم من الـ reagent وحجم من العينة، حجم
+
+689
+00:50:28,630 --> 00:50:31,510
+من الـ standard وحجم من الـ reagent، كل شغلي
+
+690
+00:50:31,510 --> 00:50:33,710
+باستخدام الـ bybit، إذا هذه صديقتنا، هذه اللي
+
+691
+00:50:33,710 --> 00:50:36,390
+هترافقنا إن شاء الله من سنة تانية لسنة رابعة لجهة
+
+692
+00:50:36,390 --> 00:50:39,950
+الأداء، فبالتالي لازم نكون دقيقين في استخدامها، و
+
+693
+00:50:39,950 --> 00:50:42,690
+عشان...عشان نكون دقيقين باستخدامها، وتكون الـ bybit
+
+694
+00:50:42,690 --> 00:50:46,370
+هادئة، احنا بنعمل لها صيانة وبنضمن دقتها باستمرار
+
+695
+00:50:46,370 --> 00:50:49,530
+لازم أنه أنا أعمل لها calibration، إيش يعني
+
+696
+00:50:49,530 --> 00:50:52,890
+calibration؟ يعني إعادة ضبط وتهيئة علشان أتأكد من
+
+697
+00:50:52,890 --> 00:50:57,070
+شغلها، إن هي عم تاخدلي شغل دقيق، طبعا كيف أنا بدي
+
+698
+00:50:57,070 --> 00:51:01,660
+آخد ال...ال...أكيد أعمل لها calibration؟ بدي
+
+699
+00:51:01,660 --> 00:51:06,200
+أستخدمها يعني، أو أظبط شغلها، أو أتأكد من إنها عم
+
+700
+00:51:06,200 --> 00:51:10,820
+تاخد volumes بشكل دقيق، باستخدام الحجوم الماء الـ
+
+701
+00:51:10,820 --> 00:51:14,960
+distilled water، تمام؟ هنا بيحكيلي إنه تحت درجة
+
+702
+00:51:14,960 --> 00:51:21,200
+الحرارة والضغط الثابت، الكثافة الماء أيضا ثابتة
+
+703
+00:51:21,200 --> 00:51:26,860
+فالحجم من الماء تمام، ممكن أنا يعني one ml من المية
+
+704
+00:51:26,860 --> 00:51:31,820
+بيعادل one gram من المية، تمام ال...الـ one ml الـ
+
+705
+00:51:31,820 --> 00:51:36,360
+volume، one ml، لو أنا جيت وزنته تحت درجة حرارة وضغط
+
+706
+00:51:36,360 --> 00:51:40,400
+ثابت وكثافة ثابتة للماء فهي...ال...الـ one ml
+
+707
+00:51:40,400 --> 00:51:46,710
+هيعطيني one gram من الـ water، تمام؟ طيب فبالتالي الـ
+
+708
+00:51:46,710 --> 00:51:51,330
+...الـ calibration للـ bypet لازم أنا أكون بس يعني
+
+709
+00:51:51,330 --> 00:51:54,390
+بدي أعرف الـ volume اللي عم تاخده، حجم الماء اللي
+
+710
+00:51:54,390 --> 00:51:59,890
+بتاخده، و هل هو accurate أو precise، وطبعا هذا
+
+711
+00:51:59,890 --> 00:52:06,910
+بيتأثر بدرجة حرارة الغرفة، وأيضا الضغط الجوي، و
+
+712
+00:52:06,910 --> 00:52:10,190
+الرطوبة، نسبة الرطوبة اللي موجودة في الوسط أو في
+
+713
+00:52:10,190 --> 00:52:14,320
+البيئة المحيطة لإجراء التجربة، عشان هيك أنا بستخدم
+
+714
+00:52:14,320 --> 00:52:17,980
+factor أو ثابت اسمه z factor في الحسابات، طبعا
+
+715
+00:52:17,980 --> 00:52:23,340
+هنحكي عنه كمان شوية، هذا الـ z factor، فالفكرة طبعا
+
+716
+00:52:23,340 --> 00:52:27,660
+إنه أنا بدي آخد one ml من الـ distilled water، يعني
+
+717
+00:52:27,660 --> 00:52:30,980
+بدي أجيب الـ by bit، بدي أعمل لها setting، بدي أ...
+
+718
+00:52:30,980 --> 00:52:35,700
+أضب...أضبطها على one ml، أركب tip، آخد أعمل الـ pre-
+
+719
+00:52:35,700 --> 00:52:39,920
+rinsing، أبلل الـ tip من...واتخلص من الـ solution
+
+720
+00:52:39,920 --> 00:52:44,350
+اللي أخدته في المرة الأولى، بعد هيك إيش بدي أعمل؟
+
+721
+00:52:44,350 --> 00:52:50,110
+بدي آخد one ml من distilled water وأحطه في cup، الـ
+
+722
+00:52:50,110 --> 00:52:53,990
+cup هذا بدي أكون مسفر عليه في الميزان، بدي أسفر
+
+723
+00:52:53,990 --> 00:52:57,590
+عليه وأحط الـ one ml وأشوف الوزنة تبعتي، الوزنة
+
+724
+00:52:57,590 --> 00:53:00,090
+تبعتي هتكون، إذا هو مسفر على الـ cup، فالوزنة اللي
+
+725
+00:53:00,090 --> 00:53:05,050
+هتطلع معايا هي وزنت الـ one ml، المفروض تكون مقاربة
+
+726
+00:53:05,050 --> 00:53:08,470
+لـ one gram، تمام؟ المفروض إنها تكون مقاربة لـ one
+
+727
+00:53:08,470 --> 00:53:13,310
+gram أو...أو يعني exactly one gram، طبعا عشان إن
+
+728
+00:53:13,310 --> 00:53:16,130
+احنا عندنا درجة الحرارة والضغط الجوي والـ
+
+729
+00:53:16,130 --> 00:53:21,070
+humidity متغيرة، فأنا بروح بدرب الوزن هذه تبعتي بالـ
+
+730
+00:53:21,070 --> 00:53:25,390
+Z factor، وفي جدول في آخر ال...ال...الـ slide
+
+731
+00:53:25,390 --> 00:53:29,590
+بعلمني كيف أنا أختار الـ Z factor المناسب اللي بدي
+
+732
+00:53:29,590 --> 00:53:36,880
+أدرب فيه القراءات تبعتي، طبعا، فإذا ال...الحجم هي هنا
+
+733
+00:53:36,880 --> 00:53:41,300
+مثلا إيش بلزمني؟ sorry، إيش بلزمني للتجربة تبعتي؟
+
+734
+00:53:41,300 --> 00:53:46,100
+بلزمني by bit وtip، بلزمني beaker أو cup لحتى أحطه
+
+735
+00:53:46,100 --> 00:53:49,220
+على الميزان وأصفر عليه، تمام؟ بلزمني distilled
+
+736
+00:53:49,220 --> 00:53:54,260
+water، بلزمني أقيس درجة الحرارة وميزان وأقيس الـ
+
+737
+00:53:54,260 --> 00:54:00,850
+pressure، الدغط عشان أطلع Z factor، أول إشي بدي أقيس
+
+738
+00:54:00,850 --> 00:54:07,510
+درجة حرارة الماء وأسجلها، بعد هيك باستخدام beaker
+
+739
+00:54:07,510 --> 00:54:10,950
+طبعا إنه أنا بدي أحطه على ال...طبعا مش راح أقرأ
+
+740
+00:54:10,950 --> 00:54:18,900
+كل الخطوات، هاي راح أختصرها باستخدام الـ beaker أو الـ
+
+741
+00:54:18,900 --> 00:54:23,080
+cup، راح أحطه على الـ analytical balance وراح أصفر
+
+742
+00:54:23,080 --> 00:54:27,080
+عليه، طبعا دي خلي أ...أ...أخلي الميزان يعتبر إن
+
+743
+00:54:27,080 --> 00:54:32,040
+وزن الـ cup هذا zero، طبعا بعد هيك بدي أعمل pre...
+
+744
+00:54:32,040 --> 00:54:35,520
+بدي أركب tip للـ bybit، وبعد ما أكون أنا حددت الحجم
+
+745
+00:54:35,520 --> 00:54:40,840
+المناسب إلها، اللي هو one ml، يعني الف microliter، و
+
+746
+00:54:40,840 --> 00:54:44,940
+بعد هيك بدي أعمل pre-rinsing، عرفنا إيش يعني pre-
+
+747
+00:54:44,940 --> 00:54:49,040
+rinsing، يعني أنا بدي أسحب أسبيرات and spins، يعني
+
+748
+00:54:49,040 --> 00:54:53,640
+بدي أسحب وأتخلص من الحجم، على أساس ممكن أعيد هذه
+
+749
+00:54:53,640 --> 00:54:56,860
+الخطوة تلت مرات، على أساس أتأكد إنه ال...ال...
+
+750
+00:54:56,860 --> 00:54:59,580
+الـ tip صارت مبللة من الداخل، يعني مش هيكون في عندي لا...لا
+
+751
+00:54:59,580 --> 00:55:05,320
+...لا فقط ولا error في الـ volume، تمام، وبعد هيك إيش
+
+752
+00:55:05,320 --> 00:55:09,880
+بدي أعمل؟ بدي أضغط على ال...ال...ال...ال...الـ
+
+753
+00:55:09,880 --> 00:55:11,120
+...ال...ال...ال...ال...ال...ال...ال...ال...
+
+754
+00:55:11,120 --> 00:55:11,380
+ال...ال...ال...ال...ال...ال...ال...ال...ال
+
+755
+00:55:11,380 --> 00:55:11,580
+...ال...ال...ال...ال...ال...ال...ال...ال...ال
+
+756
+00:55:11,580 --> 00:55:13,040
+ال...ال...ال...ال...ال...ال...ال...ال...ال
+
+757
+00:55:13,040 --> 00:55:13,160
+...ال...ال...ال...ال...ال...ال...ال...ال...ال
+
+758
+00:55:13,160 --> 00:55:13,660
+ال...ال...ال...ال...ال...ال...ال...ال...ال
+
+759
+00:55:13,660 --> 00:55:14,400
+ال...ال...ال...ال...ال...ال...ال...ال...ال
+
+760
+00:55:14,400 --> 00:55:21,420
+...ال...ال...
+
+761
+00:55:21,420 --> 00:55:21,440
+ال...ال...ال...ال...ال...ال...ال...ال...ال
+
+762
+00:55:21,440 --> 00:55:25,140
+...ال...
+
+763
+00:55:34,190 --> 00:55:40,910
+عشان السائل يفوت داخل الـ tip أيضا بشكل متأني، يعني
+
+764
+00:55:40,910 --> 00:55:44,210
+إنه ما يصير فيه اندفاع، لا solution ولا air bubble
+
+765
+00:55:44,210 --> 00:55:48,260
+والمشاكل اللي ممكن تحدث في الـ bybit، تمام؟ هيك
+
+766
+00:55:48,260 --> 00:55:51,980
+أنا أخدت الحجم اللي بدي إياه، بروح بفضيه في الـ beaker
+
+767
+00:55:51,980 --> 00:55:55,340
+أو الـ cup اللي موجود على الميزان، وبأخد القراءة
+
+768
+00:55:55,340 --> 00:55:59,760
+تمام؟ بعد هيك ممكن أنا أرجع أصفر على الـ cup والـ
+
+769
+00:55:59,760 --> 00:56:03,420
+...الـ volume اللي موجود فيه، أو إن أنا أستخدم cup
+
+770
+00:56:03,420 --> 00:56:08,100
+جديد، إيه الخيار؟ بسفر وأعيد الخطوة مرة تانية، بأخد
+
+771
+00:56:08,100 --> 00:56:13,560
+one ml بالـ bypet وأفضيه وأأخد كمان وزنة، وكمان
+
+772
+00:56:13,560 --> 00:56:17,340
+مرة تالتة، بأخذ one ml وأفضيه في الميزان، وأأخد
+
+773
+00:56:17,340 --> 00:56:22,320
+وزنة تالتة، فإذا صار في عندي تلت وزنات لتلت حجوم
+
+774
+00:56:22,320 --> 00:56:25,400
+تمام، فإيش بدي أعمل في التلت وزنات هدول؟ بدي أجيب
+
+775
+00:56:25,400 --> 00:56:28,740
+الـ average تبعهم، يعني بدي أجيب مجموعهم على تلاتة
+
+776
+00:56:29,100 --> 00:56:34,400
+تمام؟ ال...هذه ال...إيش اسمه؟ ال...يعني تكملت
+
+777
+00:56:34,400 --> 00:56:37,220
+التجربة اللي أنا حكيتها، يعني إنه...إنه عيد
+
+778
+00:56:37,220 --> 00:56:41,380
+الخطوات تلت مرات، وجيبي القراءة، هي هنا مثلا
+
+779
+00:56:41,380 --> 00:56:44,340
+بيحكيلي ممكن تعديها أربع عشرة مرات، حسب يعني ممكن
+
+780
+00:56:44,340 --> 00:56:48,200
+تعديها تلت مرات، أربع مرات، خمس مرات، المهم بالنهاية
+
+781
+00:56:48,200 --> 00:56:51,640
+الوزنات اللي تطلع معاكي بدك تاخدي لها average، إيش
+
+782
+00:56:51,640 --> 00:56:56,440
+يعني average؟ يعني مجموعها على عددها، وأطلع قراءة
+
+783
+00:56:56,440 --> 00:56:59,680
+واحدة، القراءة واحدة، هي هذه اللي بدك تروحي تعوضي
+
+784
+00:56:59,680 --> 00:57:02,940
+فيها بالقانون، الـ weight أو الـ average of weight
+
+785
+00:57:02,940 --> 00:57:06,180
+اللي طلع معاكي، وبدك تضربيه في الـ z factor اللي هو
+
+786
+00:57:06,180 --> 00:57:10,980
+معامل اللي هو الحرارة والضغط، تمام اللي موجود عندك
+
+787
+00:57:10,980 --> 00:57:14,980
+وبالنهاية بحسب الـ volume، أنا أخدت الـ bybit كانت
+
+788
+00:57:14,980 --> 00:57:18,940
+بتحكيلي إن أنا بأخذ one ml على الشاشة تبع الـ bybit
+
+789
+00:57:18,940 --> 00:57:22,840
+مكتوب one ml، فالمفروض إن هذا الكلام الـ wet في الـ
+
+790
+00:57:22,840 --> 00:57:27,020
+z factor يكون one ml أيضا، يعني أو مقارب لـ one ml
+
+791
+00:57:27,020 --> 00:57:34,050
+فممكن أنا أطبق قوانين التحليلية، يعني هي إن أنا بجيب
+
+792
+00:57:34,050 --> 00:57:39,050
+الـ average لقراءات الوزنة، اللي هي مجموع القراءات
+
+793
+00:57:39,050 --> 00:57:43,170
+على عددها، تمام، ممكن أطبق أيضا أحسب الـ standard
+
+794
+00:57:43,170 --> 00:57:47,870
+deviation، أجيب الـ accuracy، يعني القيمة الوزنة اللي
+
+795
+00:57:47,870 --> 00:57:50,610
+طلعت معايا، واللي المفروض تطلع، مثلا طلعت معايا
+
+796
+00:57:50,610 --> 00:57:54,690
+0.999 الوزنة، والمفروض إنها تطلع one milligram
+
+797
+00:57:54,690 --> 00:57:58,280
+بحسبها، بشوف...شوف نسبة الـ accuracy تبعت القيمة
+
+798
+00:57:58,280 --> 00:58:02,100
+تبعتي، والـ coefficient of variation اللي هي...
+
+799
+00:58:02,100 --> 00:58:04,560
+اللي هي عبارة عن الـ standard deviation على الـ mean
+
+800
+00:58:04,560 --> 00:58:11,920
+ضرب 100%، لازم الـ accuracy هذه تطلع من 99 لـ 101%، و
+
+801
+00:58:11,920 --> 00:58:16,470
+لازم الـ coefficient of variation يكون أقل من واحد
+
+802
+00:58:16,470 --> 00:58:19,690
+في المية، هذا يكون أقل من واحد في المية، عشان أقول
+
+803
+00:58:19,690 --> 00:58:22,290
+إن الـ by bit هذه accurate و precise وبتعطيني
+
+804
+00:58:22,290 --> 00:58:25,570
+volumes دقيقة، والحجم اللي أنا باخده هو نفسه اللي
+
+805
+00:58:25,570 --> 00:58:28,990
+موجود على الشاشة، وشغلها مية مية، إذا أنا لما بدي
+
+806
+00:58:28,990 --> 00:58:31,930
+أجري فحوصات، وبدي أنقل volume باستخدام هذه الـ by
+
+807
+00:58:31,930 --> 00:58:36,740
+bit، إذا أنا عم بشتغل بشكل صحيح وبشكل دقيق، طبعا هذا
+
+808
+00:58:36,740 --> 00:58:40,740
+الجدول اللي أنا من خلاله بختار مثلا ال...الـ z
+
+809
+00:58:40,740 --> 00:58:45,180
+value، أو اللي هو معامل ال...الحرارة والضغط، طبعا
+
+810
+00:58:45,180 --> 00:58:47,980
+الـ z value، مثلا أنا بشوف إيش الضغط اللي موجود عندي
+
+811
+00:58:47,980 --> 00:58:50,040
+حسب المنطقة اللي أنا...اللي أنا فيها، لأن أفرض
+
+812
+00:58:50,040 --> 00:58:54,620
+مثلا إن أنا موجودة في منطقة ضغطها ألف وست...سبعة
+
+813
+00:58:54,620 --> 00:58:59,420
+وستين ملي بار، طبعا ودرجة الحرارة عندي خمسة و
+
+814
+00:58:59,420 --> 00:59:04,240
+عشرين، فبروح هنا هي خمسة وعشرين، بمشي لعند مين؟ لعند
+
+815
+00:59:04,240 --> 00:59:08,100
+ال...ال...ال...المنطقة اللي هي ال...اللي فيها
+
+816
+00:59:08,100 --> 00:59:11,600
+الضغط تبعي، تمام؟ اللي فيها الضغط تبعي، هنا هيك هي
+
+817
+00:59:11,600 --> 00:59:15,480
+هذه القيمة، تقاطع درجة الحرارة اللي بدي إياها مع الضغط
+
+818
+00:59:15,480 --> 00:59:18,480
+اللي...اللي بدي إياها، هي هذه قيمة الـ Z factor اللي
+
+819
+00:59:18,480 --> 00:59:22,300
+بروح ب...ب...أطبق فيها، تمام، هي هذه قيمة الـ z
+
+820
+00:59:22,300 --> 00:59:26,060
+factor اللي بروح بأطبق فيها وبحسب حساباتي تبعتي، زي
+
+821
+00:59:26,060 --> 00:59:29,820
+ما حكينا، لو أنا عديت التجربة مرة، مرتين، تلاتة، أربع
+
+822
+00:59:29,820 --> 00:59:34,900
+عشرة، وحصلت على مثلا عشر قيم، بأخد الـ average تبعهم
+
+823
+00:59:34,900 --> 00:59:39,100
+بضربهم بالـ z factor، بحصل على الـ volume، وبأطبق في
+
+824
+00:59:39,100 --> 00:59:43,180
+القوانين تبعتي، القيمة اللي أنا جستها على القيمة
+
+825
+00:59:43,180 --> 00:59:46,570
+اللي المفروض تطلع، ضرب 100%، بحسب الـ accuracy، بحسب الـ
+
+826
+00:59:46,570 --> 00:59:50,450
+coefficient of variation، بشوف نسبة دقة وصحة ال...
+
+827
+00:59:50,450 --> 00:59:54,070
+الـ bybit بتاعي، وبحكم على شغلها، وهل إنها محتاجة
+
+828
+00:59:54,070 --> 01:00:00,690
+إنها يتم تصليحها، أو إنه لأ، مناسبة وتستخدم، يعني
+
+829
+01:00:00,690 --> 01:00:05,350
+موثوقة للاستخدام، تمام؟ بهيك بنكون خلصنا محاضرتنا
+
+830
+01:00:05,350 --> 01:00:10,030
+لليوم، إن شاء الله تكون هيك الأشياء واضحة، حاولت إن
+
+831
+01:00:10,030 --> 01:00:15,350
+أنا أورّجيكم صور، أورّجيكم فيديو لكيف الـ bybit بتاعي كنت
+
+832
+01:00:15,350 --> 
\ No newline at end of file
diff --git a/PL9fwy3NUQKwY9jl8xw-bWkVZkWcGGyspd/Vo0zfcjPiBQ_raw.json b/PL9fwy3NUQKwY9jl8xw-bWkVZkWcGGyspd/Vo0zfcjPiBQ_raw.json
new file mode 100644
index 0000000000000000000000000000000000000000..2dc8d71dcead0b8fa99bb15ecd537a53906cbbf7
--- /dev/null
+++ b/PL9fwy3NUQKwY9jl8xw-bWkVZkWcGGyspd/Vo0zfcjPiBQ_raw.json
@@ -0,0 +1 @@
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47283, 3215, 3224, 6225, 2304, 20292, 4032, 8717, 8592, 2655, 17082, 1211, 4724, 18513, 7649, 995, 35525, 19452, 24976, 10721, 3794, 5172, 8717, 19913, 23557, 5296, 11622, 32887, 5172, 9673, 5016, 1829, 9566, 3660, 8978, 8315], "avg_logprob": -0.16498162137234912, "compression_ratio": 1.7075812274368232, "no_speech_prob": 5.960464477539062e-07, "words": [{"start": 4.52, "end": 4.98, "word": "السلام", "probability": 0.7204182942708334}, {"start": 4.98, "end": 5.36, "word": " عليكم", "probability": 0.95654296875}, {"start": 5.36, "end": 5.7, "word": " ورحمة", "probability": 0.8990478515625}, {"start": 5.7, "end": 5.86, "word": " الله", "probability": 0.96240234375}, {"start": 5.86, "end": 6.48, "word": " وبركاته", "probability": 0.9904296875}, {"start": 6.48, "end": 7.18, "word": " طالبتي", "probability": 0.7059326171875}, {"start": 7.18, "end": 7.44, "word": " اليوم", "probability": 0.9150390625}, {"start": 7.44, "end": 7.58, "word": " ان", "probability": 0.7666015625}, {"start": 7.58, 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136.96, "end": 137.4, "word": " جدا", "probability": 0.99658203125}, {"start": 137.4, "end": 138.5, "word": " بمثاق", "probability": 0.834375}, {"start": 138.5, "end": 138.98, "word": " الكيميا", "probability": 0.77880859375}, {"start": 138.98, "end": 139.18, "word": " يمكن", "probability": 0.93994140625}, {"start": 139.18, "end": 139.66, "word": " أخدتوا", "probability": 0.88818359375}, {"start": 139.66, "end": 140.06, "word": " معامل", "probability": 0.9456380208333334}, {"start": 140.06, "end": 140.42, "word": " كيميا", "probability": 0.9197591145833334}, {"start": 140.42, "end": 140.7, "word": " قبل", "probability": 0.8740234375}, {"start": 140.7, "end": 141.02, "word": " هك", "probability": 0.588134765625}], "temperature": 1.0}, {"id": 8, "seek": 15616, "start": 141.31, "end": 156.17, "text": "و كنتوا تستخدموا بايبتات اللي هي زي ال glass pipe اللي كان عبارة عن بايبت glass أداة زجاجية و في آخرها أو في نهايتها بيكون في عندي مستودع من المطاط انا بتحكم بدخول و خروج الهواء و دخول و خروج السائل", "tokens": [2407, 9122, 29399, 14407, 6055, 14851, 9778, 40448, 14407, 4724, 47302, 3555, 2655, 9307, 13672, 1829, 39896, 30767, 1829, 2423, 4276, 11240, 13672, 1829, 25961, 6225, 3555, 9640, 3660, 18871, 4724, 47302, 3555, 2655, 4276, 5551, 28259, 3660, 30767, 7435, 26108, 10632, 4032, 8978, 19753, 34740, 11296, 34051, 8978, 8717, 11296, 36081, 11296, 4724, 1829, 30544, 8978, 18871, 16254, 3714, 14851, 23328, 3615, 9154, 9673, 9566, 41193, 1975, 8315, 39894, 5016, 24793, 4724, 3215, 9778, 12610, 4032, 16490, 2288, 29245, 2423, 3224, 2407, 16606, 4032, 11778, 9778, 12610, 4032, 16490, 2288, 29245, 21136, 16373, 1211], "avg_logprob": -0.16463216161355376, "compression_ratio": 1.736040609137056, "no_speech_prob": 7.152557373046875e-07, "words": [{"start": 141.31, "end": 141.55, "word": "و", "probability": 0.9833984375}, {"start": 141.55, "end": 142.05, "word": " كنتوا", "probability": 0.6705729166666666}, {"start": 142.05, "end": 142.71, "word": " تستخدموا", "probability": 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151.49, "word": " مستودع", "probability": 0.9639892578125}, {"start": 151.49, "end": 151.87, "word": " من", "probability": 0.99609375}, {"start": 151.87, "end": 152.53, "word": " المطاط", "probability": 0.884765625}, {"start": 152.53, "end": 152.73, "word": " انا", "probability": 0.762939453125}, {"start": 152.73, "end": 153.21, "word": " بتحكم", "probability": 0.9567057291666666}, {"start": 153.21, "end": 153.83, "word": " بدخول", "probability": 0.8782958984375}, {"start": 153.83, "end": 153.95, "word": " و", "probability": 0.99755859375}, {"start": 153.95, "end": 154.35, "word": " خروج", "probability": 0.8489583333333334}, {"start": 154.35, "end": 154.83, "word": " الهواء", "probability": 0.92333984375}, {"start": 154.83, "end": 154.93, "word": " و", "probability": 0.91259765625}, {"start": 154.93, "end": 155.23, "word": " دخول", "probability": 0.8855794270833334}, {"start": 155.23, "end": 155.35, "word": " و", "probability": 0.9931640625}, {"start": 155.35, "end": 155.63, "word": " 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176.04, "end": 176.52, "word": " صغيرة", "probability": 0.9949544270833334}, {"start": 176.52, "end": 176.82, "word": " جدا", "probability": 0.99560546875}, {"start": 176.82, "end": 177.0, "word": " بال", "probability": 0.98291015625}, {"start": 177.0, "end": 177.74, "word": " microliter", "probability": 0.78076171875}, {"start": 177.74, "end": 178.04, "word": " يعني", "probability": 0.9072265625}, {"start": 178.04, "end": 178.42, "word": " أقل", "probability": 0.94140625}, {"start": 178.42, "end": 178.6, "word": " من", "probability": 0.99853515625}, {"start": 178.6, "end": 178.78, "word": " one", "probability": 0.953125}, {"start": 178.78, "end": 179.14, "word": " ml", "probability": 0.96923828125}], "temperature": 1.0}, {"id": 10, "seek": 20443, "start": 179.99, "end": 204.43, "text": "فبالتالي انا بدي أداة تنقل الحجم هذا الصغير او الحجم القليل بطريقة more accurate and more precise how باستخدام ال automatic pipet اذا ال glass pipet هي تستخدم بال chemical laboratory ولكن للأسف في الحجوم الصغيرة not accurate not precise انا محتاجة أداة تكون", "tokens": [5172, 3555, 6027, 2655, 6027, 1829, 1975, 8315, 4724, 16254, 5551, 28259, 3660, 6055, 1863, 4587, 1211, 21542, 7435, 2304, 23758, 31767, 17082, 13546, 1975, 2407, 21542, 7435, 2304, 25062, 20292, 1211, 4724, 9566, 16572, 28671, 544, 8559, 293, 544, 13600, 577, 4724, 995, 14851, 9778, 3215, 10943, 2423, 12509, 8489, 302, 1975, 15730, 2423, 4276, 8489, 302, 39896, 6055, 14851, 9778, 40448, 20666, 7313, 16523, 35525, 19452, 24976, 10721, 3794, 5172, 8978, 21542, 7435, 20498, 31767, 17082, 48923, 406, 8559, 406, 13600, 1975, 8315, 3714, 33753, 26108, 3660, 5551, 28259, 3660, 6055, 30544], "avg_logprob": -0.11167763393176229, "compression_ratio": 1.662280701754386, "no_speech_prob": 7.748603820800781e-07, "words": [{"start": 179.99, "end": 180.75, "word": "فبالتالي", "probability": 0.976806640625}, {"start": 180.75, "end": 181.29, "word": " انا", "probability": 0.72802734375}, {"start": 181.29, "end": 181.55, "word": 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3607.77, "word": " تكون", "probability": 0.96044921875}, {"start": 3607.77, "end": 3608.09, "word": " هيك", "probability": 0.826904296875}, {"start": 3608.09, "end": 3608.97, "word": " الاشياء", "probability": 0.900146484375}, {"start": 3608.97, "end": 3609.51, "word": " واضحة", "probability": 0.874267578125}, {"start": 3609.51, "end": 3609.93, "word": " حولت", "probability": 0.83935546875}, {"start": 3609.93, "end": 3610.03, "word": " ان", "probability": 0.6640625}, {"start": 3610.03, "end": 3610.23, "word": " انا", "probability": 0.94482421875}, {"start": 3610.23, "end": 3611.01, "word": " اورجيكم", "probability": 0.93076171875}, {"start": 3611.01, "end": 3611.71, "word": " صور", "probability": 0.978271484375}, {"start": 3611.71, "end": 3613.23, "word": " اورجيكم", "probability": 0.90224609375}, {"start": 3613.23, "end": 3613.91, "word": " فيديو", "probability": 0.8728841145833334}, {"start": 3613.91, "end": 3614.39, "word": " لكيف", "probability": 0.87548828125}, {"start": 3614.39, "end": 3614.59, "word": " ال", "probability": 0.97900390625}, {"start": 3614.59, "end": 3614.81, "word": " by", "probability": 0.275390625}, {"start": 3614.81, "end": 3615.21, "word": " بتاعي", "probability": 0.873046875}, {"start": 3615.21, "end": 3615.35, "word": " كنت", "probability": 0.75537109375}, {"start": 3615.35, "end": 3616.17, "word": " نستخدمها", "probability": 0.88994140625}, {"start": 3616.17, "end": 3616.73, "word": " و", "probability": 0.91162109375}, {"start": 3616.73, "end": 3617.37, "word": " تعليمات", "probability": 0.9136962890625}, {"start": 3617.37, "end": 3617.51, "word": " اللي", "probability": 0.90966796875}, {"start": 3617.51, "end": 3618.09, "word": " بنتابعها", "probability": 0.8634765625}, {"start": 3618.09, "end": 3618.49, "word": " أثناء", "probability": 0.9039306640625}, {"start": 3618.49, "end": 3618.61, "word": " ال", "probability": 0.97900390625}, {"start": 3618.61, "end": 3618.81, "word": " by", "probability": 0.79638671875}, {"start": 3618.81, 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24793], "avg_logprob": -0.13219368929910189, "compression_ratio": 1.7872340425531914, "no_speech_prob": 9.655952453613281e-06, "words": [{"start": 3619.45, "end": 3621.35, "word": "لعسى", "probability": 0.8140869140625}, {"start": 3621.35, "end": 3621.73, "word": " انه", "probability": 0.73291015625}, {"start": 3621.73, "end": 3621.97, "word": " يعني", "probability": 0.833251953125}, {"start": 3621.97, "end": 3622.25, "word": " تكون", "probability": 0.9873046875}, {"start": 3622.25, "end": 3622.79, "word": " الاشي", "probability": 0.9138997395833334}, {"start": 3622.79, "end": 3623.39, "word": " مش", "probability": 0.9912109375}, {"start": 3623.39, "end": 3623.87, "word": " مبهم", "probability": 0.9378255208333334}, {"start": 3623.87, "end": 3624.59, "word": " لقلكم", "probability": 0.8323974609375}, {"start": 3624.59, "end": 3625.49, "word": " لإنه", "probability": 0.9534912109375}, {"start": 3625.49, "end": 3625.93, "word": " يعني", "probability": 0.88671875}, {"start": 3625.93, "end": 3626.35, "word": " احنا", "probability": 0.9080403645833334}, {"start": 3626.35, "end": 3626.99, "word": " مقدرين", "probability": 0.9825439453125}, {"start": 3626.99, "end": 3627.31, "word": " انه", "probability": 0.748046875}, {"start": 3627.31, "end": 3627.89, "word": " بداية", "probability": 0.9703776041666666}, {"start": 3627.89, "end": 3629.03, "word": " التخصص", "probability": 0.9774169921875}, {"start": 3629.03, "end": 3629.35, "word": " لازم", "probability": 0.9959309895833334}, {"start": 3629.35, "end": 3629.65, "word": " تكون", "probability": 0.990234375}, {"start": 3629.65, "end": 3629.81, "word": " ..", "probability": 0.56396484375}, {"start": 3629.81, "end": 3630.15, "word": " تكون", "probability": 0.96484375}, {"start": 3630.15, "end": 3630.39, "word": " يعني", "probability": 0.978759765625}, {"start": 3630.39, "end": 3630.61, "word": " كل", "probability": 0.97900390625}, {"start": 3630.61, "end": 3631.31, "word": " إشي", "probability": 0.8275553385416666}, {"start": 3631.31, "end": 3632.05, "word": " سهل", "probability": 0.9986979166666666}, {"start": 3632.05, "end": 3633.11, "word": " ومستثار", "probability": 0.681298828125}, {"start": 3633.11, "end": 3633.99, "word": " لحتى", "probability": 0.931640625}, {"start": 3633.99, "end": 3634.71, "word": " تتمكنوا", "probability": 0.98095703125}, {"start": 3634.71, "end": 3634.97, "word": " من", "probability": 0.9970703125}, {"start": 3634.97, "end": 3635.65, "word": " الفهم", "probability": 0.679443359375}, {"start": 3635.65, "end": 3636.31, "word": " المعلومة", "probability": 0.986083984375}, {"start": 3636.31, "end": 3637.03, "word": " وإدراكها", "probability": 0.8951416015625}, {"start": 3637.03, "end": 3638.13, "word": " يعطيكم", "probability": 0.8792724609375}, {"start": 3638.13, "end": 3638.39, "word": " ألف", "probability": 0.77001953125}, {"start": 3638.39, "end": 3638.97, "word": " عافية", "probability": 0.9928385416666666}, {"start": 3638.97, "end": 3639.83, "word": " وشكرا", "probability": 0.9139404296875}, {"start": 3639.83, "end": 3640.43, "word": " لكم", "probability": 0.830078125}], "temperature": 1.0}], "language": "ar", "language_probability": 1.0, "duration": 3642.23575, "duration_after_vad": 3615.1143749999974}
\ No newline at end of file
diff --git a/PL9fwy3NUQKwY9jl8xw-bWkVZkWcGGyspd/Vo0zfcjPiBQ_raw.srt b/PL9fwy3NUQKwY9jl8xw-bWkVZkWcGGyspd/Vo0zfcjPiBQ_raw.srt
new file mode 100644
index 0000000000000000000000000000000000000000..b1948ca3170a9a05df25fb1852b014e8ddcebc32
--- /dev/null
+++ b/PL9fwy3NUQKwY9jl8xw-bWkVZkWcGGyspd/Vo0zfcjPiBQ_raw.srt
@@ -0,0 +1,3360 @@
+1
+00:00:04,520 --> 00:00:07,580
+السلام عليكم ورحمة الله وبركاته طالبتي اليوم ان
+
+2
+00:00:07,580 --> 00:00:12,060
+شاء الله رح ناخد معملنا رقم اتنين بعنوان automatic
+
+3
+00:00:12,060 --> 00:00:15,600
+by-produce and calibration معمل رقم اتنين فيه مساق
+
+4
+00:00:15,600 --> 00:00:20,760
+مقدمة في التحليل الطبية طبعا هذا كان اول موضوع كان
+
+5
+00:00:20,760 --> 00:00:25,100
+المفروض ان احنا ناخده عملي و نشتغل بادانا ولكن
+
+6
+00:00:25,100 --> 00:00:30,440
+للأسف نظرا لزروف المحيطة فيناراح ناخد ان شاء الله
+
+7
+00:00:30,440 --> 00:00:33,660
+.. راح ناخد النظري تبعه و ان شاء الله بعودتنا
+
+8
+00:00:33,660 --> 00:00:38,460
+للمختبرات بناخد التطبيق العملي لإله فاليوم ان شاء
+
+9
+00:00:38,460 --> 00:00:41,060
+الله موضوعنا عن ال automatic by bit use and
+
+10
+00:00:41,060 --> 00:00:44,280
+calibration خلينا نشوف إيش يعني automatic by bit
+
+11
+00:00:44,280 --> 00:00:47,480
+هلأ احنا المحاضرة الماضية خلينا بس نحكي هالنقطة
+
+12
+00:00:47,790 --> 00:00:53,150
+المحاضرة الماضية كانت بتحكي عن ال .. ال .. ال
+
+13
+00:00:53,150 --> 00:00:58,270
+volumes و الحجوم و المحاليل و كيف أنا بتعامل مع
+
+14
+00:00:58,270 --> 00:01:01,110
+المحاليل هاي و إيش أنواع المحاليل اللي تستخدم في
+
+15
+00:01:01,110 --> 00:01:05,930
+ال medical laboratories و .. و كيف أنا أخسف هاي
+
+16
+00:01:05,930 --> 00:01:10,690
+المحاليل، تمام؟طيب لما انا .. انا بدي أجي أنقل
+
+17
+00:01:10,690 --> 00:01:13,950
+المحلول من إلى يعني مثلا انا عندي عينة بدي
+
+18
+00:01:13,950 --> 00:01:17,970
+أضيفيلها reagent علشان أعمل فحص معين أو بدي أاخد
+
+19
+00:01:17,970 --> 00:01:22,130
+.. بدي أنقل حجوم علشان أعمل dilution زي ما أخدنا
+
+20
+00:01:22,130 --> 00:01:25,800
+ال .. مسائل ال dilution المحاضرة الماضيةكيف أنا
+
+21
+00:01:25,800 --> 00:01:29,140
+بدي أنقلها؟ هنا بال medical laboratories
+
+22
+00:01:29,140 --> 00:01:32,660
+بالمختبرات الطبية احنا بنستخدم اشي اسمه automatic
+
+23
+00:01:32,660 --> 00:01:37,100
+pipet اللي هو الأداة اللي موجودة بهذه الصورة اسمها
+
+24
+00:01:37,100 --> 00:01:40,740
+automatic pipet خلينا نشوف اش يعني automatic pipet
+
+25
+00:01:41,150 --> 00:01:45,250
+هلأ automatic pipet هي عبارة عن أداة تستخدم في ال
+
+26
+00:01:45,250 --> 00:01:48,110
+biological و ال biochemical و ال microbiological
+
+27
+00:01:48,110 --> 00:01:52,310
+labs سواء كانت لل teaching purposes أو لل research
+
+28
+00:01:52,310 --> 00:01:56,490
+purposes، تمام؟ يعني تستخدم في مختبرات الأحياء و
+
+29
+00:01:56,490 --> 00:01:59,750
+مختبرات ال biochemistry و ال microbiology في كل
+
+30
+00:01:59,750 --> 00:02:04,700
+أقسام المختبرات الطبيةوتستخدم إما للتدريس أو حتى
+
+31
+00:02:04,700 --> 00:02:09,640
+للعمل لممارسة المهنة أو لأغراض البحث العلمى طبعا
+
+32
+00:02:09,640 --> 00:02:13,700
+من شأنهاي ال bybit أنها تعملى accurate transfer of
+
+33
+00:02:13,700 --> 00:02:18,500
+volume يعني أنها تنقل الحجوم بشكل دقيق جدا بمثاق
+
+34
+00:02:18,500 --> 00:02:22,050
+الكيميا يمكن أخدتوا معامل كيميا قبل هكو كنتوا
+
+35
+00:02:22,050 --> 00:02:25,250
+تستخدموا بايبتات اللي هي زي ال glass pipe اللي كان
+
+36
+00:02:25,250 --> 00:02:29,490
+عبارة عن بايبت glass أداة زجاجية و في آخرها أو في
+
+37
+00:02:29,490 --> 00:02:33,210
+نهايتها بيكون في عندي مستودع من المطاط انا بتحكم
+
+38
+00:02:33,210 --> 00:02:37,160
+بدخول و خروج الهواء و دخول و خروج السائلالى ال by
+
+39
+00:02:37,160 --> 00:02:41,280
+bit طبعا هاي ال glass by bit بتستخدم بال chemical
+
+40
+00:02:41,280 --> 00:02:45,380
+laboratory ولكن للأسف هي not accurate و not
+
+41
+00:02:45,380 --> 00:02:49,820
+precise مش accurate و مش precise متى؟ طبعا لما بدي
+
+42
+00:02:49,820 --> 00:02:53,640
+أنقل حجوم أقل من one ml و احنا هنا بالمختبرات
+
+43
+00:02:53,640 --> 00:02:57,000
+الطبية بنتعامل دائما مع حجوم صغيرة جدا بال
+
+44
+00:02:57,000 --> 00:03:01,970
+microliter يعني أقل من one mlفبالتالي انا بدي أداة
+
+45
+00:03:01,970 --> 00:03:07,230
+تنقل الحجم هذا الصغير او الحجم القليل بطريقة more
+
+46
+00:03:07,230 --> 00:03:11,170
+accurate and more precise how باستخدام ال
+
+47
+00:03:11,170 --> 00:03:15,470
+automatic pipet اذا ال glass pipet هي تستخدم بال
+
+48
+00:03:15,470 --> 00:03:19,670
+chemical laboratory ولكن للأسف في الحجوم الصغيرة
+
+49
+00:03:19,670 --> 00:03:25,060
+not accurate not precise انا محتاجة أداة تكونأكثر
+
+50
+00:03:25,060 --> 00:03:28,560
+دقة more accurate more precise لهيك أنا بستخدم ال
+
+51
+00:03:28,560 --> 00:03:32,200
+automatic by bit لإن كل شغلي بيكون على ال volumes
+
+52
+00:03:32,200 --> 00:03:37,480
+اللي أقل من one ml طبعا طيب كل by bit من هدول ال
+
+53
+00:03:37,480 --> 00:03:42,440
+automatic by bit مصممة إن هي تنقل يعني مدى أو
+
+54
+00:03:42,440 --> 00:03:47,050
+range من الحجوم من إلىيعني في بايبت متكون مصممة
+
+55
+00:03:47,050 --> 00:03:50,930
+تنقل من واحد microliter لعشرة microliter في من ..
+
+56
+00:03:50,930 --> 00:03:54,770
+مصممة بتكون من عشرة microliter لمية microliter في
+
+57
+00:03:54,770 --> 00:03:58,610
+من مية microliter ل one ml تمام ف .. و في من one
+
+58
+00:03:58,610 --> 00:04:04,050
+ml لخمسة ml فإذا ال .. في بايبتات أنواع و حجوم و
+
+59
+00:04:04,050 --> 00:04:08,390
+كل بايبت بتكون إلها range يعني مدى من إلى
+
+60
+00:04:08,390 --> 00:04:15,140
+بتستخدموا في ال .. ال .. ال ..نقل الحجوم هذه و
+
+61
+00:04:15,140 --> 00:04:19,600
+طبعا احنا المفروض احنا مانتخطى هذا ال .. ال .. ال
+
+62
+00:04:19,600 --> 00:04:23,820
+range تبعها يعني لازم انا مثلا اذا بدي انقل خمسين
+
+63
+00:04:23,820 --> 00:04:28,320
+microliter يفضل ان انا اخد بايبت ال range تبعها
+
+64
+00:04:28,320 --> 00:04:31,480
+بحيث انه الخمسين تكون واقعة فيه ال range في داخل
+
+65
+00:04:31,480 --> 00:04:34,980
+هذا ال range يعني مثلا باخد بايبتبتنقل من عشرة
+
+66
+00:04:34,980 --> 00:04:38,420
+microliter لمية microliter ال volume رقم خمسين
+
+67
+00:04:38,420 --> 00:04:42,580
+هيكون already موجود يعني في المدى و ال by بتحتقدر
+
+68
+00:04:42,580 --> 00:04:46,880
+انها تنقلي هذا ال volume فبالتالي انا هكون ضمنت ال
+
+69
+00:04:46,880 --> 00:04:50,250
+accuracy و ال precision لناقل ال volumesطيب إذا
+
+70
+00:04:50,250 --> 00:04:54,850
+إحنا نوّهنا إنه أنا في عندي أنواع وحجوم ويعني قدرة
+
+71
+00:04:54,850 --> 00:04:58,930
+استيعابية لال buy bits يعني مش كلها واحد مش كلها
+
+72
+00:04:58,930 --> 00:05:03,930
+مصممة إنها تنقلني نفس الحجم طيب فإذا في عندي حاجة
+
+73
+00:05:03,930 --> 00:05:08,010
+اسمها buy bit man model طبعا هي سبع أنواع من ال
+
+74
+00:05:08,010 --> 00:05:12,440
+buy bits مصممة إنها تنقل حجوم بطريقة مختلفةطبعا
+
+75
+00:05:12,440 --> 00:05:17,560
+هذه ال .. ال man model يعني لو هم السبعة بيكونوا
+
+76
+00:05:17,560 --> 00:05:20,980
+بينقلولي من point one microliter يعني من اقل من
+
+77
+00:05:20,980 --> 00:05:24,680
+واحد microliter لحد خمس تلاف microliter يعني لحد
+
+78
+00:05:24,680 --> 00:05:30,100
+خمس ML يعني في تنوع من ال by بتات هذه و هلأ بنشوف
+
+79
+00:05:30,100 --> 00:05:35,090
+بالجدوة اللي في ال slide ال ..التالية هيوضحلي إيش
+
+80
+00:05:35,090 --> 00:05:38,970
+يعني by bit مع الموديل ولكن هنا بيحكيلنا إنه the
+
+81
+00:05:38,970 --> 00:05:42,710
+most often used يعني الأكثر استخداما هي ال by
+
+82
+00:05:42,710 --> 00:05:49,150
+bitات اللي تسمى ال B20 و ال B200 و ال B1000 هدول
+
+83
+00:05:49,150 --> 00:05:54,150
+ال models هم الأكثر استخداما اللي بينقلولي حجم من
+
+84
+00:05:54,150 --> 00:05:58,450
+اتنين لعشرينو من خمسين ل متين و من مية ل ألف
+
+85
+00:05:58,450 --> 00:06:02,170
+microliter تمام هاي المدى تبعهم respectively مرتب
+
+86
+00:06:02,170 --> 00:06:07,410
+بالترتيب ال B20 بتنقل من اتنين ل عشرين microliter
+
+87
+00:06:07,410 --> 00:06:12,990
+تمام و ال بايبت B20 بتنقل volume من خمسين ل متين
+
+88
+00:06:12,990 --> 00:06:16,730
+microliter و البايبت الف بتاخد من مية ل الف
+
+89
+00:06:16,730 --> 00:06:20,970
+microliter يعني الرقم اللي جنب ال B تمام بي يعني
+
+90
+00:06:20,970 --> 00:06:25,240
+بايبت و الرقم هذا يعني أقصى قيمة بتقدر تاخدهاطبعا
+
+91
+00:06:25,240 --> 00:06:28,800
+ومش .. مش أدنى قيمة لأ فى أدنى .. الأدنى قيمة غير
+
+92
+00:06:28,800 --> 00:06:32,080
+مذكورة هلأ نيجى نشوف ال buy bet man model هى فى
+
+93
+00:06:32,080 --> 00:06:36,960
+عندى واحد اتنين أربع ستة سبع أنواع من ال buy bet
+
+94
+00:06:36,960 --> 00:06:43,870
+طبعا ومسمى بي تو يعنى بتنقل من zero لتنين طبعاو ال
+
+95
+00:06:43,870 --> 00:06:51,010
+B20 من 0 ل 10 بتاخد ال B20 بتاخد من 0 ل 20 ال B100
+
+96
+00:06:51,010 --> 00:06:55,250
+بتاخد من 0 ل 100 و 200 من 0 ل 200 و هكذا من 0 ل
+
+97
+00:06:55,250 --> 00:07:00,570
+1000 يعني من 1 أمال و هنا من 0 ل 5000 لحد one ..
+
+98
+00:07:00,570 --> 00:07:04,310
+لحد خمس أمال هذا ال range بصي بالهذا اللي بيكون
+
+99
+00:07:04,310 --> 00:07:07,690
+مسجل على ال by بت نفسها يعني بيكون .. بيكون ..
+
+100
+00:07:07,690 --> 00:07:12,960
+بيعطين ال range انه من .. من كذا لكذاأنا لما بدى
+
+101
+00:07:12,960 --> 00:07:16,040
+أستخدم ال by bet هذه طبعا انا مابستخدم ال by bet
+
+102
+00:07:16,040 --> 00:07:18,700
+عشان أنقله zero volume يعني انا مابديش أنقل volume
+
+103
+00:07:18,700 --> 00:07:21,840
+ليش أستخدم ال by bet؟ zero volume يعني no volume
+
+104
+00:07:21,840 --> 00:07:26,160
+طبعا فإذا في عندي حاجة اسمها recommended range
+
+105
+00:07:26,160 --> 00:07:29,600
+يعني ال range الموصفة يعني أنا بستخدم ال by bet
+
+106
+00:07:29,600 --> 00:07:36,260
+لحتى انه انا يعني في range مسموحلي فيه ايش هو اللي
+
+107
+00:07:36,260 --> 00:07:42,040
+مسموحلي فيه؟ من point واحد لاتنين طبعاو ال .. و
+
+108
+00:07:42,040 --> 00:07:45,880
+هنا من نص لعشرة من اتنين لعشرين من عشرة لمية و
+
+109
+00:07:45,880 --> 00:07:50,400
+هكذا من خمسين لمتان طيب هذه ال by bet اللي من
+
+110
+00:07:50,400 --> 00:07:53,780
+خمسين لمتان ممكن تسحب لأقل من خمسين اه ممكن تسحب
+
+111
+00:07:53,780 --> 00:07:56,560
+لأقل من خمسين ال range تبعها اصلا من zero لمتان
+
+112
+00:07:56,900 --> 00:08:00,800
+ولكن هو اذا سحبت اقل من خمسين فهي بتكون inaccurate
+
+113
+00:08:00,800 --> 00:08:05,200
+and imprecise يعني مش accurate ومش precise فانا
+
+114
+00:08:05,200 --> 00:08:08,300
+يفضل ان انا استخ .. اخت .. يعني اتبع ال
+
+115
+00:08:08,300 --> 00:08:13,460
+recommended range علشان أستخ .. أختار ال .. ال ..
+
+116
+00:08:13,460 --> 00:08:18,170
+ضيبة المناسبة لإلي تمام؟طيب يعني مثلا لو أنا بدي
+
+117
+00:08:18,170 --> 00:08:23,030
+أنقل خمسين microliter تمام؟ خمسين microliter مين
+
+118
+00:08:23,030 --> 00:08:29,190
+أفضل أختار ال .. ال by بت مثلا رقم ال B100 ولا ال
+
+119
+00:08:29,190 --> 00:08:33,410
+B200؟ يفضل أن أنا أختار ال B100 لإنه الخمسين
+
+120
+00:08:33,410 --> 00:08:36,570
+القيمة خمسين تقع في نص ال range يعني أنا ب .. بكون
+
+121
+00:08:36,570 --> 00:08:41,710
+ضمنة إنه مافيش أخطاءتمام ال by بت على ال border
+
+122
+00:08:41,710 --> 00:08:45,850
+على الحدود تمام ممكن يكون فيها نسبة خطأ لكن في
+
+123
+00:08:45,850 --> 00:08:49,950
+منتصف ال range بتكون دقيقة فإذا انا بفضل ان انا
+
+124
+00:08:49,950 --> 00:08:54,810
+لما بدي اختار اللي بدي انقل volume بختار ال by بت
+
+125
+00:08:54,810 --> 00:08:57,870
+اللي هذا ال volume يتوسط ال range تبعها او موجود
+
+126
+00:08:57,870 --> 00:09:02,120
+في ال range تبعهاطبعا طيب هلأ لو بدي أنقل مثلا ميت
+
+127
+00:09:02,120 --> 00:09:08,300
+microliter مين أفضل أختار ال B100 طبعا ولا ال B200
+
+128
+00:09:08,300 --> 00:09:15,620
+ولا ال B1000 يفضل أن أنا أختار ال B200 طبعا و ممكن
+
+129
+00:09:15,620 --> 00:09:18,560
+أنا أختار ال B100 بتكون دقيقة يعني ال higher range
+
+130
+00:09:18,560 --> 00:09:23,040
+تبعتها بتكون أدق دايما طبعا ال higher range بتكون
+
+131
+00:09:23,040 --> 00:09:26,680
+أدق من ال lower range لل bybit طبعا يعني هذه ال
+
+132
+00:09:26,680 --> 00:09:32,120
+bybit ال B100يعني لو بدأ أنقل 100 microliter من ال
+
+133
+00:09:32,120 --> 00:09:37,620
+volume ممكن أستخدم ال B200 ال .. ال .. ال B100
+
+134
+00:09:37,620 --> 00:09:43,440
+ممكن أستخدم ال B1000 تمام؟ و .. لكن مثلا هذه ال by
+
+135
+00:09:43,440 --> 00:09:47,500
+bit اللي هو ال range تبعها من عشرة لمية تمام؟ عند
+
+136
+00:09:47,500 --> 00:09:50,800
+المية بتكون أضاق من من .. من .. من أني أسحب فيها
+
+137
+00:09:50,800 --> 00:09:54,640
+عشرة microliter يعني دايما ال higher limit تبعتها
+
+138
+00:09:54,640 --> 00:09:57,990
+بتكون أضاق من ال lower limit لل by bit نفسهاطبعا
+
+139
+00:09:57,990 --> 00:10:02,750
+إذا نرجع نعيد الصياغة تاني لو أنا في عندى .. بدي
+
+140
+00:10:02,750 --> 00:10:05,690
+أنقل 100 microliter مين أفضل أستخدم؟ الأفضل أن أنا
+
+141
+00:10:05,690 --> 00:10:09,990
+أستخدم ال B200 لإنها المية تتوسط ال range ممكن
+
+142
+00:10:09,990 --> 00:10:13,730
+أستخدم ال B100 لإنه حكينا ال higher limit بتكون
+
+143
+00:10:13,730 --> 00:10:16,990
+أدق دايما من ال lower limit لل by bit ولكن أنا
+
+144
+00:10:16,990 --> 00:10:20,110
+مابستخدم .. يعني ممكن أستخدمها بس مابكنش دقيقة و
+
+145
+00:10:20,110 --> 00:10:24,170
+.. و .. كتير لإنه دايما حكينا إنه ال lower limit
+
+146
+00:10:24,170 --> 00:10:27,370
+لل by bit بتكون أقل دقة من ال higher limitهذا
+
+147
+00:10:27,370 --> 00:10:31,690
+بالنسبة ل .. ال .. ال .. ال by bit man model حكينا
+
+148
+00:10:31,690 --> 00:10:36,770
+مين هم اكتر شيوعا و اكتر استخداما بي twenty و ال
+
+149
+00:10:36,770 --> 00:10:41,910
+by one hundred و ال by two hundred تمام؟ اذا ال by
+
+150
+00:10:41,910 --> 00:10:46,570
+عشرين ال by مية و ال by متين هم اكتر ال .. ال by
+
+151
+00:10:46,570 --> 00:10:50,570
+بتات شيوعا و استخداماتمام طيب هلا بنا نحكي عن
+
+152
+00:10:50,570 --> 00:10:56,150
+أنواع .. يعني sorry مكونات البيبت و أجزاقها طيب
+
+153
+00:10:56,150 --> 00:11:00,430
+البيبت زي ما انتوا شايفين بهذا الشكل مكونة من حاجة
+
+154
+00:11:00,430 --> 00:11:05,030
+اسمها ال .. البش .. البشبطن أو البلنجر نحكي عنه
+
+155
+00:11:05,030 --> 00:11:09,770
+إما بشبطن أو بلنجر إيش يعني بشبطن أو بلنجر؟زي ما
+
+156
+00:11:09,770 --> 00:11:13,250
+انتوا شايفين هذا ال push button هو عبارة عن كبسة
+
+157
+00:11:13,250 --> 00:11:18,830
+push انا ممكن انا اضغط عليها و تطلع و تنزل من ال
+
+158
+00:11:18,830 --> 00:11:24,030
+by button تمام؟ و هذا الرد تبعها طبعا هذا الرد ال
+
+159
+00:11:24,030 --> 00:11:29,980
+.. ال .. ال .. ال ..القضيب هذا ممكن انه يلتف دائري
+
+160
+00:11:29,980 --> 00:11:35,500
+علشان يتحكم في ال volume تمام؟ يتحكم في ال volume
+
+161
+00:11:35,500 --> 00:11:39,620
+اللي موجودة على ال digital screen هنا تمام؟ هلأ في
+
+162
+00:11:39,620 --> 00:11:45,240
+هنا adapter موجود هذا يعني علشان انا اقلل من حركة
+
+163
+00:11:45,240 --> 00:11:47,800
+ال vibe يعني ممكن انا اسبط هذا ال adapter على
+
+164
+00:11:47,800 --> 00:11:51,880
+أصبعي .. على أصبعي السبابة وامسكها بشكل انه يكون
+
+165
+00:11:51,880 --> 00:11:58,230
+انا متحكمة فيها ومسكاها بشكل صحيحهذا بسميه tip
+
+166
+00:11:58,230 --> 00:12:04,410
+eject .. tip ejector أو tip eject button تمام؟ هو
+
+167
+00:12:04,410 --> 00:12:09,730
+عبارة عن كبسة بتتخلص من ال tip يعني بتتحرك .. مجرد
+
+168
+00:12:09,730 --> 00:12:14,360
+ما أضغط عليها ال tip اللي هنا بتفكطبعا هذا عبارة
+
+169
+00:12:14,360 --> 00:12:19,020
+عن ال tip ejector و هنا tip holder و زي ما انتوا
+
+170
+00:12:19,020 --> 00:12:23,560
+شايفين هنا في مكان لأ ال handle من هنا بمسكها و
+
+171
+00:12:23,560 --> 00:12:29,220
+هنا الشاشة اللي بتكون موجود عليها القراءة بتكون
+
+172
+00:12:29,220 --> 00:12:35,070
+موجود عليها القراءة تبعتيطيب، هلأ بالنسبة لل .. ال
+
+173
+00:12:35,070 --> 00:12:39,110
+.. بايبت هي بتيجي تمام لحالها هيك و فيه انا عندي
+
+174
+00:12:39,110 --> 00:12:43,370
+حاجة اسمها tips او بايبت tip طبعا هذه ال .. بايبت
+
+175
+00:12:43,370 --> 00:12:48,400
+tip هي عبارة عن قطع بلاستيكيةهذه زي ما انتوا
+
+176
+00:12:48,400 --> 00:12:54,520
+شايفين في الصورة هنا هي عبارة عن قطع بلاستيكية لت
+
+177
+00:12:54,520 --> 00:12:59,980
+.. ت .. يعني بتتركب على ال .. تستخدم لبيبتات و هي
+
+178
+00:12:59,980 --> 00:13:02,960
+تتراوح في الأحجام تبعتها يعني البيبتات الصغيرة
+
+179
+00:13:02,960 --> 00:13:06,900
+بيكون إلها تبات، بيكون إلها تب صغيرة، البيبتات
+
+180
+00:13:06,900 --> 00:13:11,630
+الأكبر بيكون إلها تب أكبر و هكذاهي قطع بلاستيكية و
+
+181
+00:13:11,630 --> 00:13:16,170
+هذه القطع البلاستيكية بستخدمها انا one use ايش
+
+182
+00:13:16,170 --> 00:13:20,090
+يعني one use يعني بستستخدم لمرة واحدة فقط لنقل حجم
+
+183
+00:13:20,090 --> 00:13:24,210
+لمرة واحدة فقط و بعد هيك انا بتلفها بتخلص منها
+
+184
+00:13:24,210 --> 00:13:29,490
+طبعا فإذا مابرجع استخدمها مرة تانية فهذه هي التبات
+
+185
+00:13:29,490 --> 00:13:35,870
+او القطع اللي بتتركب على ال by bit وبيكون الحجم او
+
+186
+00:13:35,870 --> 00:13:39,550
+ال volume داخلها يعني انا السائل اللي انا بنقله
+
+187
+00:13:39,700 --> 00:13:44,480
+بيكون موجود داخل هاي التب وبيتم ال .. ال .. نقله
+
+188
+00:13:44,480 --> 00:13:48,760
+من مكان إلى آخر حسب أنا وين بدي أو كيف بدي أشتغل
+
+189
+00:13:48,760 --> 00:13:55,780
+طيب، إذا هاي مكونات البايبت و أجزاقها و يعرفنا إنه
+
+190
+00:13:55,780 --> 00:14:00,250
+التب هي قطعة خارجيةتتركب لل by bet مش ثابتة في ال
+
+191
+00:14:00,250 --> 00:14:05,490
+by bet و انه هذه القطعة ايضا ممكن اني استخدمها
+
+192
+00:14:05,490 --> 00:14:09,210
+لمرة واحدة فقط يعني بتلفها بعد ما استخدمها خلاص
+
+193
+00:14:09,210 --> 00:14:13,430
+برميها و اذا هذه يطلق عليها مصطلح disposable
+
+194
+00:14:13,430 --> 00:14:19,040
+disposable يعني استخدام لمرة واحدة فقطوحكينا ايضا
+
+195
+00:14:19,040 --> 00:14:24,320
+انه هذه ال .. ال .. التب يعني بتتنقل .. بيكون
+
+196
+00:14:24,320 --> 00:14:27,980
+حجمها متناسب مع ال bybit ال by بتاعة اللي بتنقل
+
+197
+00:14:27,980 --> 00:14:31,780
+حجوم صغيرة بيكون لها تب صغيرة و ال by بتاعة اللي
+
+198
+00:14:31,780 --> 00:14:37,440
+بتنقل حجم أكبر بيكون لها تب حجمه أكبر نوعا ماطيب
+
+199
+00:14:37,440 --> 00:14:40,980
+فيه هنا نصائح لاستخدام ال micro bibit اللي هي
+
+200
+00:14:40,980 --> 00:14:45,120
+عبارة عن إيش هذه النصائح؟ أول حاجة never exceed
+
+201
+00:14:45,120 --> 00:14:49,140
+the upper or lower limit of this bibit يعني أنه
+
+202
+00:14:49,140 --> 00:14:55,710
+يعني مثلا هاي ال bibit هايبتنقللي مثلا من عشرة
+
+203
+00:14:55,710 --> 00:15:00,350
+لمية، يعني ممنوع أنا أسحب أقل من عشرة و ممنوع أسحب
+
+204
+00:15:00,350 --> 00:15:04,590
+فيها أكتر من مية، يعني ما أسحب أقل من عشرة ولا
+
+205
+00:15:04,590 --> 00:15:09,350
+أكتر من مية، يعني ما أتخطأ ال range هادا، إما بأقل
+
+206
+00:15:09,350 --> 00:15:13,840
+منه أو بأكتر منههذا معناه الجملة هاى الجملة هاى
+
+207
+00:15:13,840 --> 00:15:18,300
+معناها انه never exceed ما اتخطى ال upper او ال
+
+208
+00:15:18,300 --> 00:15:22,320
+lower limit لل range لل by bit ال by bit يعني
+
+209
+00:15:22,320 --> 00:15:28,180
+مصممة بأنها تنقلي داخل range معين فانا باحترم هذا
+
+210
+00:15:28,180 --> 00:15:32,880
+ال range بلتزم فيه ما بستخدمها ال by bit الاقل منه
+
+211
+00:15:32,880 --> 00:15:37,900
+ولا الاكثر منه على نقل حجوم علشان ما اكون بنقل
+
+212
+00:15:37,900 --> 00:15:42,840
+حجوم بطريقة مش precise و مش accurateتمام طيب so
+
+213
+00:15:42,840 --> 00:15:46,840
+the desired volume by turning centrally located
+
+214
+00:15:46,840 --> 00:15:49,700
+ring clockwise to increase volume or
+
+215
+00:15:49,700 --> 00:15:53,120
+counterclockwise to decrease احنا حكينا انه هذا
+
+216
+00:15:53,120 --> 00:15:59,290
+القضيب او ال .. ال .. ال ..الرد تمام هذا بيتحرك
+
+217
+00:15:59,290 --> 00:16:02,530
+بشكل دائري و من خلاله انا بتحكم بالحجم اللى موجود
+
+218
+00:16:02,530 --> 00:16:06,290
+على الشاشة هنا تمام يعني من خلاله انا بحدد الحجم
+
+219
+00:16:06,290 --> 00:16:11,450
+اللى بديها بحكيلك هنا انه تحركيه ال ال ال
+
+220
+00:16:11,450 --> 00:16:18,530
+clockwise تمام و عشان يزيد الحجم و بنحركه counter
+
+221
+00:16:18,530 --> 00:16:25,520
+clockwise to decrease ال volumeطبعا انا هنا بالشكل
+
+222
+00:16:25,520 --> 00:16:29,140
+او بالتجربة العملية لما بنشرح احنا كيف نستخدم ال
+
+223
+00:16:29,140 --> 00:16:32,720
+bybit في المعامل انا بخل ال bybit .. بخل الطالبة
+
+224
+00:16:32,720 --> 00:16:37,120
+تمسك ال bybit تمام و بحيث انه ال digital screen
+
+225
+00:16:37,120 --> 00:16:41,300
+تكون مواجهةلإلها تمام يعني تكون عينها على ال
+
+226
+00:16:41,300 --> 00:16:45,800
+digital screen وبالتالي بما أنه بيكون عينها على ال
+
+227
+00:16:45,800 --> 00:16:49,660
+digital screen إذا حركت هذا الرد بشكل دائري
+
+228
+00:16:49,660 --> 00:16:54,260
+باتجاهها يعني لعندها تمام هيك بيزيد ال volume أما
+
+229
+00:16:54,260 --> 00:16:59,180
+إذا حركته باتجاه المعاكس يعني بعيد عنها فهو بيقل
+
+230
+00:16:59,180 --> 00:17:02,340
+ال volume تمام هذا بده ممارسة ان شاء الله بنشرحه
+
+231
+00:17:02,340 --> 00:17:09,020
+بالطريقة أو بالتجربة العمليةتمام؟ هذا بالنسبة ل ..
+
+232
+00:17:09,020 --> 00:17:14,320
+ال .. ال .. ال by bet و الطرق أو اللي هي الخطوات
+
+233
+00:17:14,320 --> 00:17:17,740
+أو اللي .. ال .. التعليمات اللي لازم أنا ألتزم
+
+234
+00:17:17,740 --> 00:17:22,920
+فيها أثناء السحب أو نقل الحجوم باستخدام ال by bet
+
+235
+00:17:23,850 --> 00:17:29,330
+في شغل هنا انه ال .. ال by bit هنا طبعا هي بتكون
+
+236
+00:17:29,330 --> 00:17:33,210
+مصممة انها تنقلي او تقفز قفزات في ال volume يعني
+
+237
+00:17:33,210 --> 00:17:38,970
+مثلا هاي هنا بحكيلي انه هي صح يعني من خمسين ل متين
+
+238
+00:17:38,970 --> 00:17:45,210
+بتسحب لكن انه مثلا انا لما بدي ..أحرك بصير بحرك
+
+239
+00:17:45,210 --> 00:17:48,450
+بيزيد ال volume كل شوية point اتنين point اتنين
+
+240
+00:17:48,450 --> 00:17:51,890
+لحد ما اصل لل volume اللي بديها لذا هذه هي ال ..
+
+241
+00:17:51,890 --> 00:17:55,450
+ال .. مدى التحريك او اللي هي القافزات في ال
+
+242
+00:17:55,450 --> 00:18:01,330
+volumes طبعا طيب بليت ا tip place a tip on the
+
+243
+00:18:01,330 --> 00:18:05,230
+discharge end of the pipet طبعا انا ب .. بركب ال
+
+244
+00:18:05,230 --> 00:18:10,370
+tip في المكان المخصص لإلها و بعد هيك طبعا ب ..
+
+245
+00:18:12,600 --> 00:18:16,600
+باستخدام البلانجر ب .. باستخدمه علشان اسحب ال
+
+246
+00:18:16,600 --> 00:18:20,520
+volumes هذا البلانجر اللي احنا شايفينه تمام مش ما
+
+247
+00:18:20,520 --> 00:18:24,180
+.. ايضا هو يعني فايد .. فايديته مش بس انه متصل
+
+248
+00:18:24,180 --> 00:18:28,360
+بالرد high بال .. و بتحكم بال volume لأ هذا
+
+249
+00:18:28,360 --> 00:18:31,600
+البلانجر احنا كتركم push button يعني انا ممكن اكدس
+
+250
+00:18:31,600 --> 00:18:35,480
+عليا بيطلع و بينزلطبعا انا هذا لما بدي أكبس عليه و
+
+251
+00:18:35,480 --> 00:18:39,860
+يطلع و ينزل بينزل عند ضغطتين، الضغطة الأولى لما
+
+252
+00:18:39,860 --> 00:18:43,840
+أضغط الضغطة الأولى بيقللي ال exact volume اللي
+
+253
+00:18:43,840 --> 00:18:50,720
+موجود ع الشاشة تمام؟ لما أضغط ضغطتين تمام؟بينقلي
+
+254
+00:18:50,720 --> 00:18:54,280
+extra volume ممكن يكون حجم مضاعف او extra volume
+
+255
+00:18:54,280 --> 00:18:59,560
+من ال volume اللي مصمم او موجود على الشاشة تمام؟
+
+256
+00:18:59,560 --> 00:19:04,400
+اذا هذا ال bush هذا البلنجر عبارة عن كبسة فيها two
+
+257
+00:19:04,400 --> 00:19:07,680
+stops يعني انا لما اضغط اول ضغطة بلاحظ انه خلاص
+
+258
+00:19:07,680 --> 00:19:12,780
+وجه اصبعي لدرجة معينة وانا بضغط لو ضغطت بقوة اكبر
+
+259
+00:19:12,780 --> 00:19:16,910
+ممكن اضغط الضغطة التانية يعني بنزل كمان مرةفالضغطة
+
+260
+00:19:16,910 --> 00:19:21,410
+الأولى لما أنا بكون ضغطاها بتكون ال by بتها تسحبلي
+
+261
+00:19:21,410 --> 00:19:25,750
+volume نفس ال volume اللى موجودة ع الشاشة لكن لو
+
+262
+00:19:25,750 --> 00:19:29,690
+أنا بدى أسحب two volumes او يعني بصري بدى أسحب و
+
+263
+00:19:29,690 --> 00:19:33,110
+أنا ضغطة ضغطتين هيسحبلي ال volume اللى موجودة ع
+
+264
+00:19:33,110 --> 00:19:37,610
+الشاشة وكمان extra volume تانى يعني volume بزيادة
+
+265
+00:19:37,610 --> 00:19:40,710
+طب انا ليش امتى بستخدم الضغطة الأولى و امتى بستخدم
+
+266
+00:19:40,710 --> 00:19:45,100
+الضغطة التانية او الضغطتين هلأ بنشوف كدههي هنا
+
+267
+00:19:45,100 --> 00:19:47,920
+بيحكيلي انه البلانجر او اللي هو ال push button
+
+268
+00:19:47,920 --> 00:19:53,140
+تمام و ال stop at two different positions يعني هذا
+
+269
+00:19:53,140 --> 00:19:57,740
+البلانجر بيتوقف عند .. عند مكانين و انا لما اجي
+
+270
+00:19:57,740 --> 00:20:00,820
+اضغط عليه بلاحظ انه في عندي two resistance في
+
+271
+00:20:00,820 --> 00:20:05,020
+مقاومتين للضغطة تبعتي ال .. ال stop الأول او
+
+272
+00:20:05,020 --> 00:20:10,160
+الضغطة الأولى تمام و لو انا زودت ال .. الضغط تبعي
+
+273
+00:20:10,160 --> 00:20:16,630
+بنزل كمان ضغطة تانية فبالتاليال .. ال .. ال first
+
+274
+00:20:16,630 --> 00:20:21,870
+stopping تمام عند ال initial resistant تمام عند
+
+275
+00:20:21,870 --> 00:20:27,610
+أول يعني stop عند أول إعاقة لضغطة تبعتي بيجف ال ..
+
+276
+00:20:27,610 --> 00:20:30,850
+ال plunger عندها بتكون هذه عبارة عن الضغطة الأولى
+
+277
+00:20:31,230 --> 00:20:37,590
+طبعا و ال .. و هذه معناها انه انا هسحب ايش ال
+
+278
+00:20:37,590 --> 00:20:40,810
+desired volume يعني انا هاخد ال volume اللي موجود
+
+279
+00:20:40,810 --> 00:20:47,720
+ع الشاشة exactly باخده طبعا ال second stoppingلما
+
+280
+00:20:47,720 --> 00:20:53,380
+يكون .. لما انا بدي اضغط على الضغط التاني يعني انا
+
+281
+00:20:53,380 --> 00:20:58,900
+بدي اظل نازلة في البلانجر ضغطين فساعتها انا بكون
+
+282
+00:20:58,900 --> 00:21:02,540
+تجاوزت ال initial resistance عند .. تجاوزت الإعاقة
+
+283
+00:21:02,540 --> 00:21:06,540
+الأولى و انزلت كمان مرة في ال .. في البلانجرطبعا
+
+284
+00:21:06,540 --> 00:21:12,600
+فهدى ال .. الاشي معناها انه ال by bit راح تسحبلي
+
+285
+00:21:12,600 --> 00:21:17,940
+volume extra طبعا هتسحبلي ال .. ال volume extra
+
+286
+00:21:17,940 --> 00:21:22,720
+ساعتها انا لما بلقل الحجم بفضيه بضغط ضغطة واحدة
+
+287
+00:21:22,950 --> 00:21:26,350
+هتنقل الحجم و هيضل في عندي extra volume موجود في
+
+288
+00:21:26,350 --> 00:21:31,130
+ال tip ممكن أتخلص منه تمام؟ اذا في عندي ال .. ال
+
+289
+00:21:31,130 --> 00:21:34,370
+.. بلانجر ال .. او ال .. ال push button بيجف عن
+
+290
+00:21:34,370 --> 00:21:38,430
+ضغطين، الدغطة الأولى بتن .. لما انا أكون ضغطاها و
+
+291
+00:21:38,430 --> 00:21:42,270
+أسحب حجم من السائل معناها أنا سحبت نفس الحجم اللي
+
+292
+00:21:42,270 --> 00:21:45,600
+موجودة على الشاشةلكن اذا انا كنت ضغطة ضغطتين و
+
+293
+00:21:45,600 --> 00:21:50,120
+سحبت حجم معناها انا سحبت الحجم مع extra تمام لما
+
+294
+00:21:50,120 --> 00:21:54,040
+اجي افضية بدي انتبه ان انا اضغط ضغطة واحدة فقط
+
+295
+00:21:54,040 --> 00:21:57,520
+افضي ال volume اللي من ضغطة واحدة لان ضغطة واحدة
+
+296
+00:21:57,520 --> 00:22:00,380
+يعني ايش ال desired volume يعني ال volume اللي
+
+297
+00:22:00,380 --> 00:22:04,580
+بديها و بيضل في عندي extra طبعا طب ليش الغلبة هذه
+
+298
+00:22:04,580 --> 00:22:07,880
+ليش ضغطة واحدة و ضغطتين و ليش انا اخد extra volume
+
+299
+00:22:08,030 --> 00:22:11,550
+علشان في عندي نوعين من ال by betting نوع اسمه
+
+300
+00:22:11,550 --> 00:22:16,450
+forward by betting و reverse by betting ايش يعني
+
+301
+00:22:16,450 --> 00:22:19,770
+forward by betting يعني انه انا افوت في المحلول
+
+302
+00:22:19,770 --> 00:22:23,870
+تمام و انا ضغطة ضغطة واحدة اخد الحجم اللي بدياه هو
+
+303
+00:22:23,870 --> 00:22:27,390
+نفس الحجم اللي موجود على الشاشة exactly بالضبط
+
+304
+00:22:27,390 --> 00:22:32,530
+بدون زيادة و بعد هيك اجي افضيه افضيه في المحلول في
+
+305
+00:22:32,530 --> 00:22:37,350
+الوعاء اللي انا بدي اشتغل فيه و بفضيه و بضل ضغطة
+
+306
+00:22:37,540 --> 00:22:40,020
+ممكن اضغط اكتر من مرة او ممكن اضغط اكتر من مرة او
+
+307
+00:22:40,020 --> 00:22:44,880
+ممكن اضغط اكتر من مرة او ممكن اضغط اكتر من مرة او
+
+308
+00:22:44,880 --> 00:22:48,820
+ممكن اضغط اكتر من مرة او ممكن اضغط اكتر من مرة او
+
+309
+00:22:48,820 --> 00:22:48,960
+ممكن اضغط اكتر من مرة او ممكن اضغط اكتر من مرة او
+
+310
+00:22:48,960 --> 00:22:49,180
+ممكن اضغط اكتر من مرة او ممكن اضغط اكتر من مرة او
+
+311
+00:22:49,180 --> 00:22:49,860
+ممكن اضغط اكتر من مرة او ممكن اضغط اكتر من مرة او
+
+312
+00:22:49,860 --> 00:22:52,000
+ممكن اضغط اكتر من مرة او ممكن اضغط اكتر من مرة او
+
+313
+00:22:52,000 --> 00:22:53,780
+ممكن اضغط اكتر من مرة اإذا إيش ال forward
+
+314
+00:22:53,780 --> 00:22:57,380
+bybiting؟ معناها .. معناها إن أنا أضغط ضغطة على ال
+
+315
+00:22:57,380 --> 00:23:01,680
+bybit بعدين أجي أس .. أ .. أنقل الحجم أاخد الحجم
+
+316
+00:23:01,680 --> 00:23:04,200
+اللي بدياه هو ال exact volume اللي موجود على
+
+317
+00:23:04,200 --> 00:23:08,120
+الشاشة و لما أجي أفضيه في الوعاء ممكن أضغط ضغط أو
+
+318
+00:23:08,120 --> 00:23:11,040
+ضغطتين عشان أتأكد إن أنا فضيته كله و إن أنا
+
+319
+00:23:11,040 --> 00:23:14,700
+استخدمت ال volume بالضبط هو نفس اللي موجود على
+
+320
+00:23:14,700 --> 00:23:17,720
+الشاشة تمام؟ مايكونش ضاي .. إيش يضاي العالق في ال
+
+321
+00:23:17,720 --> 00:23:23,070
+tip تمام؟ إذا ..هذا معناه ال forward وهذه الطريقة
+
+322
+00:23:23,070 --> 00:23:27,410
+تستخدم عشان انا لو بدى انقل حجوم زى مين زى ال
+
+323
+00:23:27,410 --> 00:23:31,450
+aqueous solution المحليل اللى أصلها مية ال buffers
+
+324
+00:23:31,450 --> 00:23:35,310
+ال acid ال bases كل الأشياء المحليل هذه اللى أصلها
+
+325
+00:23:35,310 --> 00:23:39,750
+مية بستخدم معاها طريقة ال forward by bettingأما
+
+326
+00:23:39,750 --> 00:23:43,390
+طريقة ال reverse by betting فهي عبارة عن انه انا
+
+327
+00:23:43,390 --> 00:23:47,990
+باخد larger volume of solution بصحب larger volume
+
+328
+00:23:47,990 --> 00:23:51,990
+of solution ايش يعني؟ يعني بصحب extra يعني معناها
+
+329
+00:23:51,990 --> 00:23:55,430
+انا لما باجي باخد ال by bet او بنقل في ال by bet
+
+330
+00:23:55,430 --> 00:24:00,870
+بكون ضغطة، ضغطتين تمام؟ باخد الحجم اللي بديها مع
+
+331
+00:24:00,870 --> 00:24:05,750
+extra تمام؟و لما اجى افضى فى المحلول بتغضى تواحدة
+
+332
+00:24:05,750 --> 00:24:11,030
+بحيث ان انا افضى فقط المحلول الحجم اللى بديه و يضل
+
+333
+00:24:11,030 --> 00:24:14,330
+ال extra اللى موجود فى التب اتخلص منه طب انا ليش
+
+334
+00:24:14,330 --> 00:24:20,430
+يعني بلجأ لهذه الطريقة بحكيلي لإنه فى سوائل عبارة
+
+335
+00:24:20,430 --> 00:24:25,250
+عن لازجة viscous طبعا هاد اش يعني viscous لازجة
+
+336
+00:24:25,250 --> 00:24:29,130
+يعني بصعوبة هتطلع جوا التب المحليل بصعوبة هتطلع
+
+337
+00:24:29,130 --> 00:24:35,520
+جوا التب طبعا طيبفبالتالي لما اجي انا اكون ماخدة
+
+338
+00:24:35,520 --> 00:24:39,200
+ال volume وزيادة شوية هكون ضامنة ان انا اخدت ال
+
+339
+00:24:39,200 --> 00:24:42,060
+volume ..يعني ضامنة ان انا هنزل ال volume على
+
+340
+00:24:42,060 --> 00:24:47,480
+الأقل اللي بديها، تمام؟ لكن مثلا لو انا ده استخدمت
+
+341
+00:24:47,480 --> 00:24:52,430
+مع الهادي المحلي للviscousاستخدمت نظامة ضغطة لوحدة
+
+342
+00:24:52,430 --> 00:24:57,050
+ما هي بتطلع جوا التب بطء يعني انا ممكن افوت و اطلع
+
+343
+00:24:57,050 --> 00:25:01,590
+و اخد ال باقي الحجم اللي بدياه بس لسه السائل من
+
+344
+00:25:01,590 --> 00:25:08,200
+كتر لزوجته ماعباش جوا التب بشكلكامل فبالتالي ممكن
+
+345
+00:25:08,200 --> 00:25:12,800
+اخد volume اقل من اللي انا بديها فهنا ممكن يكون في
+
+346
+00:25:12,800 --> 00:25:17,120
+خطأ عشان هيك بحكيلي انه استخدمي مع السوائل اللازجة
+
+347
+00:25:17,120 --> 00:25:21,200
+انه طريقة انك انت تاخدي بزيادة و لما تفض تفضي
+
+348
+00:25:21,200 --> 00:25:27,230
+الحجم اللي بدكياتمام هذا بالنسبة لمين ل .. ال ..
+
+349
+00:25:27,230 --> 00:25:31,150
+ال .. السواقل اللازجة بستخدم معاها ال reverse
+
+350
+00:25:31,150 --> 00:25:34,330
+technique يعني نظام الضغطين عشان أخد extra volume
+
+351
+00:25:34,330 --> 00:25:37,590
+أيضا بيحكيلي فيه محاليل volatile إيش يعني
+
+352
+00:25:37,590 --> 00:25:42,030
+volatile؟ يعني متطايرة يعني مجرد مثلا ما أسحبها في
+
+353
+00:25:42,030 --> 00:25:46,350
+ال pipe و أنقلها لل .. للوعائل عشان أفضيها ممكن
+
+354
+00:25:46,350 --> 00:25:50,810
+تكون اتبخرت فهنا بيصير في عندي فاقد في الحجم
+
+355
+00:25:50,810 --> 00:25:58,290
+فبالتالي أنا بكون نقلت حجمأقل من اللازم فهنا باع
+
+356
+00:25:58,290 --> 00:26:05,490
+هذه النوع من السوائل ايضا ممكن انه استخدم نظام
+
+357
+00:26:05,490 --> 00:26:09,230
+الضغطين بحيث ان انا اخد حجم بزيادة و لما افضل افضل
+
+358
+00:26:09,230 --> 00:26:14,030
+exact volume عشان اذا مهما يكون الطاير من هذا ال
+
+359
+00:26:14,030 --> 00:26:18,050
+solution اكون على الأقل مازلة محتفظة بال volume
+
+360
+00:26:18,050 --> 00:26:21,890
+اللي انا بديهاتمام؟ أيضا في بعض المحاليل اللي
+
+361
+00:26:21,890 --> 00:26:24,510
+بنسميها foaming solution ايش يعني foaming
+
+362
+00:26:24,510 --> 00:26:28,950
+solution؟ يعني محاليل بتعمل رغبة تمام؟ فيها مادة
+
+363
+00:26:28,950 --> 00:26:33,190
+صابون او يعني مادة ..الها ملمة الصابونة هذه
+
+364
+00:26:33,190 --> 00:26:37,690
+المحاليل اللي فيها مواد ذات ملمة الصابونة تمام؟
+
+365
+00:26:37,690 --> 00:26:41,120
+ممكن تعملي رغبةو بالتالي لما أجي أصحب أصحب من
+
+366
+00:26:41,120 --> 00:26:45,220
+الرغبة high فبالتالي أكون نقلت volume أقل من
+
+367
+00:26:45,220 --> 00:26:49,520
+اللازم لإن أنا يعني جزء من الرغبة احتلت ال tub
+
+368
+00:26:49,520 --> 00:26:53,300
+تمام؟ و أفكر حالي إن أنا أخدت الحجم اللي بدياه و
+
+369
+00:26:53,300 --> 00:26:59,700
+فعليا أنا بكون ماخدة حجم أقل من ال ..المفروض
+
+370
+00:26:59,700 --> 00:27:03,300
+فبالتالي بستخدم برضه نظام ال larger volume of
+
+371
+00:27:03,300 --> 00:27:06,120
+solution يعني ان انا بدي اخد ال extra volume يعني
+
+372
+00:27:06,120 --> 00:27:09,700
+بدي استخدم ال reverse technique يعني نظام ضغطتين و
+
+373
+00:27:09,700 --> 00:27:17,300
+.. و بعد هيك شسمه بروح بفضي بضغطة واحدةتمام؟ عشان
+
+374
+00:27:17,300 --> 00:27:21,780
+لو كان في رغبة في المحلول أكون ضامنة أنها ما تأثر
+
+375
+00:27:21,780 --> 00:27:25,360
+على الحجم اللي أنا بدي أنقله تمام؟ و أيضا عشان لو
+
+376
+00:27:25,360 --> 00:27:29,180
+أنا بدي أنقل حجوم صغيرة جدا جدا يعني أنا خايفة ..
+
+377
+00:27:29,180 --> 00:27:32,260
+يعني مش متأكدة أخدت ال volume ولا لأ لأنه في حجوم
+
+378
+00:27:32,260 --> 00:27:35,600
+يعني زي مثلا حجم one microliterيعني انت حتى ما
+
+379
+00:27:35,600 --> 00:27:39,040
+بتشوفيه على التب يعني صعب تشوفيه على التب انه انت
+
+380
+00:27:39,040 --> 00:27:42,720
+أخدتي او ما أخدتي فالأفضل ان انا ااخد حجم بزيادة و
+
+381
+00:27:42,720 --> 00:27:45,560
+لما افضي داخل ال solution او المقلوعه اللي انا بدي
+
+382
+00:27:45,560 --> 00:27:50,640
+اشتغل فيه بفضي بالحجم اللي بديها اذا .. اذا لما
+
+383
+00:27:50,640 --> 00:27:56,550
+انا اسحب الحجمبضغطة و افضيه بدغطتين معناها انا بدي
+
+384
+00:27:56,550 --> 00:28:01,050
+اخدت exact volume و افضيته بدغطتين عشان اضمن ان
+
+385
+00:28:01,050 --> 00:28:05,270
+انا افضيته كويس تمام هذه طريقة forward bibuting
+
+386
+00:28:05,270 --> 00:28:10,510
+لكن لما انا اسحب الحجم بدغطتين و افضيه بدغطة واحدة
+
+387
+00:28:10,510 --> 00:28:14,590
+معناها انا استخدمت طريقة ال reverse bibuting و هذا
+
+388
+00:28:14,590 --> 00:28:20,490
+طبعا بيختلفهلأ ال .. ال .. اذا هذه ال bybit شايفين
+
+389
+00:28:20,490 --> 00:28:24,570
+هي البلانجر بالكبس عليه في عندى ضغطة واحدة او
+
+390
+00:28:24,570 --> 00:28:28,690
+ضغطتان depress the blanger until you feel the
+
+391
+00:28:28,690 --> 00:28:32,090
+initial resistance مجرد ما انت تضغطي طبعا ان شاء
+
+392
+00:28:32,090 --> 00:28:35,670
+الله راح نرجع على المعامل و امسكهم ال bybit كل بنت
+
+393
+00:28:35,670 --> 00:28:39,770
+راح يكونيلها bybitو تشوف و تجرب كيف تشتغل عليها
+
+394
+00:28:39,770 --> 00:28:43,130
+كيف تضغط .. كيف لما بتضغط بتقابل ال resistant
+
+395
+00:28:43,130 --> 00:28:46,750
+الأولى اللي هي الإعاقة الأولى يعني إشي بيعيط مصاري
+
+396
+00:28:46,750 --> 00:28:50,590
+ال blanket إشي بيوقفه هذه بتكون الدغطة الأولى و
+
+397
+00:28:50,590 --> 00:28:55,390
+بقى لو .. لو كبست بقوة أكبر بتكون ساعتها أخدت ال
+
+398
+00:28:55,390 --> 00:28:59,730
+.. ال .. ال vibe أخدت الدغطتين تمام فيعني بصير ده
+
+399
+00:28:59,730 --> 00:29:05,530
+تسحب extra volumeطيب فبالتالي لما احنا بدنا نفوت
+
+400
+00:29:05,530 --> 00:29:09,090
+في ال .. ال .. ال vibe طبعا لما اجي افوتها بفوتها
+
+401
+00:29:09,090 --> 00:29:13,190
+بحيث انها تكون مغموقة جزء منها مغمور او جزء من
+
+402
+00:29:13,190 --> 00:29:18,330
+التب مغمور داخل ال solution تمام؟ مابروح اخلي
+
+403
+00:29:18,330 --> 00:29:23,290
+الفتحة التب او هنا يعني الفوهة التب ملامسة للسطح
+
+404
+00:29:23,290 --> 00:29:26,450
+لإنه انا ممكن اسحب سائل و هوا مع بعض مدام ها على
+
+405
+00:29:26,450 --> 00:29:31,690
+السطحو أيضا كمان ما بخليها تلامس البطم لل solution
+
+406
+00:29:31,690 --> 00:29:36,630
+ليش؟ لإن هذه الفوهة أو فتحة التب ممكن يغلقها اللي
+
+407
+00:29:36,630 --> 00:29:43,810
+هي قاع ال container او ال tube فبالتالي انه انا
+
+408
+00:29:43,810 --> 00:29:48,550
+بكون هي كعملت انسداد للتب و مش راح اسحب ال volume
+
+409
+00:29:48,550 --> 00:29:52,730
+اللي بدي يعني تمام؟ اذا بضغطال .. الضغطة أولة أو
+
+410
+00:29:52,730 --> 00:29:57,510
+ضغطة تانية حسب نوع ال .. ال .. ال bybiting لو أنا
+
+411
+00:29:57,510 --> 00:30:01,890
+بستخدم forward bybiting هضغط ضغطة واحدة تمام بفوت
+
+412
+00:30:01,890 --> 00:30:04,830
+ال tip بحيث أنه يكون جزء منها مغمور داخل ال
+
+413
+00:30:04,830 --> 00:30:08,050
+solution مش على السطح ولا على .. في القاع م ..
+
+414
+00:30:08,050 --> 00:30:12,630
+ملامسة للقاع و ببدأ بعد هيك إيش أعمل أرفع إيدي
+
+415
+00:30:12,630 --> 00:30:18,350
+بالتدريج عن البلانجر بالتدريج تمام مهم جدا أن أنا
+
+416
+00:30:18,350 --> 00:30:22,860
+أرفع إيدي بالتدريج عن البلانجرمارفعش هيك فجأة
+
+417
+00:30:22,860 --> 00:30:28,380
+واحدة ليش؟ لإن لو رفعت فجأة راح يندفع ال solution
+
+418
+00:30:28,380 --> 00:30:33,520
+بقوة داخل ال .. ال .. التب فهذا هيسببلي أخطاء ممكن
+
+419
+00:30:33,520 --> 00:30:37,340
+ما أخد ال volume اللي بديه ممكن ال .. ال .. السائل
+
+420
+00:30:37,340 --> 00:30:40,940
+يندفع ويفوت داخل ال pipe ذات نفسها تمام؟ يعني يطلع
+
+421
+00:30:40,940 --> 00:30:44,560
+من التب ويفوت داخل ال pipe ويقدي إلى الصدق ل ..
+
+422
+00:30:44,560 --> 00:30:49,380
+لإلها أو إلى التلف تمام؟ وممكن أكون يعني يسحب هوا
+
+423
+00:30:49,750 --> 00:30:52,750
+معاها يعني يكون فيه فقاعات فبالتالي يكون فيه error
+
+424
+00:30:52,750 --> 00:30:56,830
+في ال volume اللي نقلته فبالتالي يفضل انه انا لما
+
+425
+00:30:56,830 --> 00:31:00,910
+اجي ارفع البلانجر ارفعه بالتدريج عشان المحلول يفوت
+
+426
+00:31:00,910 --> 00:31:05,710
+بالتدريج او ببطء داخل ال pipet ويفوت بشكل دقيق و
+
+427
+00:31:05,710 --> 00:31:11,220
+اكون انا عم باخد الحجم الدقيق تمام؟بعد هيك بروح
+
+428
+00:31:11,220 --> 00:31:15,120
+على ال container التاني و ب .. أضغط على البلانجر
+
+429
+00:31:15,120 --> 00:31:19,720
+مرتين يعني على ال initials ال resistant و بفضيه و
+
+430
+00:31:19,720 --> 00:31:22,320
+ممكن أضغط على ال second resistant عشان أتأكد أن
+
+431
+00:31:22,320 --> 00:31:26,040
+أنا فضيت كل ال volume و بعد هيك بتخلص من ال tip
+
+432
+00:31:26,040 --> 00:31:29,080
+كيف بتخلص من ال tip؟ حكينا أنه في هنا على الطرف
+
+433
+00:31:29,080 --> 00:31:33,720
+حاجة اسمها ejector لل tipهنا مجرد ما انا اضغط على
+
+434
+00:31:33,720 --> 00:31:37,500
+هذا ال ejector لل tip ال tip بتكون خلاص فانفصلت عن
+
+435
+00:31:37,500 --> 00:31:43,100
+ال pipet و انا بتخلص منها فلما بدي اتخلص من ال tip
+
+436
+00:31:43,100 --> 00:31:47,520
+بوجه ال pipet هذه باتجاه السلة تمام و بضغط على ال
+
+437
+00:31:47,520 --> 00:31:53,860
+ejector فلحالها ال pipet بتروح على الفضلات
+
+438
+00:31:54,650 --> 00:31:59,550
+ع الوسط طبعا هذا بالنسبة ل .. ال .. ال forward
+
+439
+00:31:59,550 --> 00:32:05,230
+بالنسبة ل reverse طبعا يعني تقريبا نفس التعليمات
+
+440
+00:32:05,230 --> 00:32:08,270
+ولكن انه انا لما بدي افوت ضغطة بدي اكون ضغطة
+
+441
+00:32:08,270 --> 00:32:13,250
+ضغطتين و بدي افوت ب .. و اكون جزء من الطب مغمور
+
+442
+00:32:13,250 --> 00:32:17,510
+داخل ال solution طبعا بفوت بشكل عمودي يا بنات لو
+
+443
+00:32:17,510 --> 00:32:20,710
+انا بدي افوت بشكل مائل بيأثر على الحجم اللي انا
+
+444
+00:32:20,710 --> 00:32:24,510
+مخدعةبفوت بشكل عمودي داخل ال solution جزء من ال
+
+445
+00:32:24,510 --> 00:32:29,230
+tip مغمور داخل ال solution و برفع ايدي بالتدريج عن
+
+446
+00:32:29,230 --> 00:32:32,670
+البلنجر بسحبلي ال volume طبعا هنا في ال reverse
+
+447
+00:32:32,670 --> 00:32:37,190
+انا ضغطت ضغطتين فهيسحبلي extra volume تمام؟بعدين
+
+448
+00:32:37,190 --> 00:32:40,570
+بروح على ال container اللي انا بدي افض فيه و بضغط
+
+449
+00:32:40,570 --> 00:32:44,230
+ضغطة واحدة علشان تنزلي ال solution اللي انا بديها
+
+450
+00:32:44,230 --> 00:32:48,210
+فقط الحجم اللي بديها فقط و ال extra هو و ال tab
+
+451
+00:32:48,210 --> 00:32:50,730
+بتخلص منه في ال west من خلال ان انا اضغط على ال
+
+452
+00:32:50,730 --> 00:32:54,590
+ejector و تنفصل ال tab عن ال pipet و تروح في حال
+
+453
+00:32:54,590 --> 00:33:00,370
+سبيلهمتمام؟ أول حاجة مع .. يعني ملاحظات لاستخدام
+
+454
+00:33:00,370 --> 00:33:04,650
+ال bybit, never point a bybit up ممنوع أنك انت
+
+455
+00:33:04,650 --> 00:33:07,970
+تستخدمي ال bybit تقليبيها بهذا الشكل بحيث انها
+
+456
+00:33:07,970 --> 00:33:13,600
+تكون ال tip صارت فوق و ال bybit تحتطبعا و انا
+
+457
+00:33:13,600 --> 00:33:16,300
+دايما لما بستخدم ال bible بتكون بهذا الاتجاه بحيث
+
+458
+00:33:16,300 --> 00:33:19,620
+أنه التب لا تحت لإنه لو أنا بدي أجلبها ال volume
+
+459
+00:33:19,620 --> 00:33:22,680
+اللي موجود في التب راح يفوت داخل ال bible طبعا هذه
+
+460
+00:33:22,680 --> 00:33:28,420
+عبارة عن أنا بيب طبعا و في كمان نظام ال .. ال ..
+
+461
+00:33:28,420 --> 00:33:33,460
+الذنبركاتفبالتالي ممكن تصدق داخل ال .. يعني يصير
+
+462
+00:33:33,460 --> 00:33:37,300
+صدق او يصير فيه تلف و ممكن في محاليل بترسب بتعمل
+
+463
+00:33:37,300 --> 00:33:41,440
+رواس بتعمل انسداد فهذا بيأثر على دقة ال pipet فهو
+
+464
+00:33:41,440 --> 00:33:45,340
+يفضل ان انا ممنوع اوجهها الى اعلى بحيث ان ال
+
+465
+00:33:45,340 --> 00:33:48,580
+liquid يفوت داخل ال pipet و يعملها destroying
+
+466
+00:33:48,580 --> 00:33:53,060
+تمام؟طيب when with a drawing liquid with with a
+
+467
+00:33:53,060 --> 00:33:56,540
+pipe it always release the plunger slowly طبعا
+
+468
+00:33:56,540 --> 00:34:00,720
+لحظة لاحظته انتوا حتى بنبرت صوتي ركزتي كتير منيح و
+
+469
+00:34:00,720 --> 00:34:04,020
+انا بحكيلكم لما ترفع ايدك عن ال plunger ارفعيه
+
+470
+00:34:04,020 --> 00:34:09,040
+slowly شويه شويه بالتدريج بهدوء مابرفع ايدي فجأة
+
+471
+00:34:09,040 --> 00:34:13,800
+ليش لإن هنا هي بحكيلك انه ال .. ال .. ال ..الشغل
+
+472
+00:34:13,800 --> 00:34:19,280
+رافعت ايدك بشكل تدريجي او بشكل بطيء عن البلانجر
+
+473
+00:34:19,280 --> 00:34:24,080
+هيمنع من انه ال liquid يصير له rushing into انه
+
+474
+00:34:24,080 --> 00:34:28,160
+يندفع داخل ال pipe و بالتالي ممكن يسببلها انسداد
+
+475
+00:34:28,160 --> 00:34:35,100
+او صدق او تلف فبالتاليمهم جدا طبعا انه ارفع ايدي
+
+476
+00:34:35,100 --> 00:34:38,020
+بالتدريج this is especially important with large
+
+477
+00:34:38,020 --> 00:34:41,860
+volumes يعني و هذا الاشي بنتبهله كتير منيح و انا
+
+478
+00:34:41,860 --> 00:34:46,740
+بصحب حجوم كبيرة حجوم كبيرة معناها السائل هيندفع
+
+479
+00:34:46,740 --> 00:34:50,720
+بشكل قوي هو كميته كبيرة فهيفوت داخل ال bipe هيصل ل
+
+480
+00:34:50,720 --> 00:34:55,240
+ال bipe من جوا و يتلفها be sure you use the proper
+
+481
+00:34:55,240 --> 00:35:02,780
+size tip for each bipe حكينا انه لكلبيبت طبعا لكل
+
+482
+00:35:02,780 --> 00:35:08,040
+بيبت في عندى تب خاصة فيها تبعا لحجمها أو تبعا ل ال
+
+483
+00:35:08,040 --> 00:35:12,380
+range تبعها فانا كل بيبت بستخدملها التب الخاصة
+
+484
+00:35:12,380 --> 00:35:15,340
+فيها ما بنفع مثلا مع البيبتات الصغيرة أستخدم تب
+
+485
+00:35:15,340 --> 00:35:19,040
+كبيرة أصلا مش راح تركب عليها وما بنفع مع البيبتات
+
+486
+00:35:19,040 --> 00:35:21,980
+الكبيرة أستخدم تب صغيرة مش راح تكفي لل volume اللي
+
+487
+00:35:21,980 --> 00:35:26,520
+أنا بدني أنقله طبعا فإذا كل بيبت لها تب خاصة فيها
+
+488
+00:35:26,520 --> 00:35:30,740
+بستخدمهاalways use a new tip for each different
+
+489
+00:35:30,740 --> 00:35:35,960
+liquid طبعا كل حجم بدي أنقله بستخدمله a new tip
+
+490
+00:35:35,960 --> 00:35:40,020
+طبعا ال tip جديدة و حكينا انه هاي ال tip أشمالها
+
+491
+00:35:40,020 --> 00:35:44,460
+disposable طبعا فهيمر معانا ان شاء الله مصطلح
+
+492
+00:35:44,460 --> 00:35:48,360
+disposable و أذكركم فيه يعني تستخدم لمرة واحدة فقط
+
+493
+00:35:48,640 --> 00:35:52,580
+طيب use the correct pipet for the volume to be
+
+494
+00:35:52,580 --> 00:35:56,260
+dispended يعني انا بدي استخدم ال pipet اختار ال
+
+495
+00:35:56,260 --> 00:36:02,180
+pipet اللي ال range تبعها تمام مناسب ل .. ال .. ال
+
+496
+00:36:02,180 --> 00:36:04,980
+.. ال pipet ل الحجم اللي بدي أنقله هى مثلا هنا
+
+497
+00:36:04,980 --> 00:36:09,420
+بيحكيلك never use the متين ل الف microliter pipet
+
+498
+00:36:09,420 --> 00:36:12,720
+to dispense متين تمام هى هنا مثلا هى ال range من
+
+499
+00:36:12,720 --> 00:36:18,210
+متين ل الف مابستخدمها عشان انقل حجم متينلأن حكينا
+
+500
+00:36:18,210 --> 00:36:20,870
+إن هي مش هتكون دقيقة على lower limit لكن ممكن تكون
+
+501
+00:36:20,870 --> 00:36:23,630
+دقيقة على upper limit أو بتكون أدقهي على upper
+
+502
+00:36:23,630 --> 00:36:28,750
+limit تمام؟ و لو أنا بدي أخد حجوم أقل من 200 أو
+
+503
+00:36:28,750 --> 00:36:33,790
+أكتر من ألف هذا إيش ممكن يعملي سببلي damage للأداة
+
+504
+00:36:33,790 --> 00:36:39,710
+هذه فبالتالي لازم أكون حذرة و حريصة في استخدام ال
+
+505
+00:36:39,710 --> 00:36:44,850
+bybit without the bybitwithdraw the pipet from the
+
+506
+00:36:44,850 --> 00:36:48,290
+receiving vessels carefully sliding the tip along
+
+507
+00:36:48,290 --> 00:36:51,890
+the wall هلأ مجرد ما انا ارفع ال pipet بعد ما اخد
+
+508
+00:36:51,890 --> 00:36:55,610
+الحجم اللي بديا لازم اعمل حاجة اسمها sliding يعني
+
+509
+00:36:55,610 --> 00:37:00,750
+انا امرر ال tip هادي امسحها على فتحة ال container
+
+510
+00:37:00,750 --> 00:37:05,690
+يعني مثلابهذا الشكل يعني ارفع وانزل ال buy bet على
+
+511
+00:37:05,690 --> 00:37:11,130
+اللي فوهت او فتحت ال container تبع المحلول بحيث
+
+512
+00:37:11,130 --> 00:37:15,910
+انه اي اشي عالق على جدار ال tip من برا اتخلص منه
+
+513
+00:37:15,910 --> 00:37:19,330
+انا بعنيني فقط ال volume اللي انا نقلته في الداخل
+
+514
+00:37:19,330 --> 00:37:23,670
+طبعا فبالتالي عشان ما اخد excess volume remove the
+
+515
+00:37:23,670 --> 00:37:30,770
+tip from the sample liquidتمام؟ و no liquid should
+
+516
+00:37:30,770 --> 00:37:35,130
+remain on the outside of the tip طبعا يعني لما ..
+
+517
+00:37:35,130 --> 00:37:40,130
+مجرد ما انا اطلع من ال .. ال .. يعني اخد الحجم
+
+518
+00:37:40,130 --> 00:37:43,410
+اللي بديها و اطلع من ال container مش لازم يضل
+
+519
+00:37:43,410 --> 00:37:47,910
+بقايا من ال solution على ال tip فيفضل ان انا امسح
+
+520
+00:37:47,910 --> 00:37:52,620
+باستخدام ال wipe تمام؟او ال .. ال جوز او اي قطعة
+
+521
+00:37:52,620 --> 00:37:58,780
+.. قطعة ت .. clinics او قطن او شاش باجفف ال .. ال
+
+522
+00:37:58,780 --> 00:38:02,780
+.. ال bybit بمسحها بمررها عليها بحيث ان انا اجفف
+
+523
+00:38:02,780 --> 00:38:07,300
+اي بقايا عالقة عليها ولكن يف .. بكون حريصة انه
+
+524
+00:38:07,300 --> 00:38:12,600
+هادي ال .. ال .. ال vibe تمامما تلامس فوهة التب
+
+525
+00:38:12,600 --> 00:38:17,540
+ليش لإنها لو لامست فوهة التب هذا absorbent المحارم
+
+526
+00:38:17,540 --> 00:38:21,000
+و ال clinics و الاشي بينطس السائل فممكن تمطس
+
+527
+00:38:21,000 --> 00:38:25,820
+السائل اللي موجود داخل التب و بالتالي بينقص ال
+
+528
+00:38:25,820 --> 00:38:28,560
+volume اللي أخدته و بيكون أنا هيك عم باخد volumes
+
+529
+00:38:28,560 --> 00:38:32,260
+مش accurate و مش precise تمام مهم جدا لها هاي
+
+530
+00:38:32,260 --> 00:38:37,440
+الشغل اذا بعد هى مجرد ما إنه أسحب ال .. ال .. ال
+
+531
+00:38:37,440 --> 00:38:37,860
+.. ال .. ال .. ال .. ال .. ال .. ال .. ال .. ال ..
+
+532
+00:38:37,860 --> 00:38:38,800
+ال .. ال .. ال .. ال .. ال .. ال .. ال .. ال .. ال
+
+533
+00:38:38,800 --> 00:38:39,540
+.. ال .. ال .. ال .. ال .. ال .. ال .. ال .. ال ..
+
+534
+00:38:39,540 --> 00:38:39,680
+ال .. ال .. ال .. ال .. ال .. ال .. ال .. ال .. ال
+
+535
+00:38:39,680 --> 00:38:39,680
+.. ال .. ال .. ال .. ال .. ال .. ال .. ال .. ال ..
+
+536
+00:38:39,680 --> 00:38:43,650
+ال .. المش لازم no liquid should remain on the
+
+537
+00:38:43,650 --> 00:38:46,950
+outside مش لازم ال liquid يضل على خارج ال tip
+
+538
+00:38:46,950 --> 00:38:51,470
+فبالتالي بمسح قطارات اللي .. اللي موجودة على ال
+
+539
+00:38:51,470 --> 00:38:56,190
+outside of the tip بستخدام tissue, paper, جوز,
+
+540
+00:38:56,730 --> 00:39:01,130
+wipes أي إش .. أي نوع محارب أو clinics بحيث انه ما
+
+541
+00:39:01,130 --> 00:39:04,790
+ألامس never touch the tip orifice مافتحق ماألامسش
+
+542
+00:39:04,910 --> 00:39:08,290
+ال tip orifice يعني فتحة ال .. ال tip ال opening
+
+543
+00:39:08,290 --> 00:39:12,410
+تبع ال tip طبعا ماقلامسه بحيث انه ماينطست السائل و
+
+544
+00:39:12,410 --> 00:39:16,770
+بهك انا اكون عم باخد volume اقل من اللازم و اكون
+
+545
+00:39:16,770 --> 00:39:19,870
+مش .. مش accurate و مش precise في عملية ال volume
+
+546
+00:39:19,870 --> 00:39:25,090
+transfer طبعا طيب pre-rinsing ايش يعني كلمة pre
+
+547
+00:39:25,090 --> 00:39:31,610
+-rinsing يفضل انه انا .. انا لما بدي انقل حجوم ان
+
+548
+00:39:31,610 --> 00:39:39,390
+انا ابلليعني مثلا هنا يفضل إن أنا أبلل ال .. ال ..
+
+549
+00:39:39,390 --> 00:39:44,290
+التب من جوا في .. في .. في السائل اللي بدي أنقله،
+
+550
+00:39:44,290 --> 00:39:47,510
+هاي معناه pre runs، طبعا؟ يعني أول حاجة بصحب أول
+
+551
+00:39:47,510 --> 00:39:52,330
+volume و بتخلص منه بكبه، هيك أنا بضمن إنه جزء من
+
+552
+00:39:52,330 --> 00:39:56,570
+السائل لمس ال .. ال .. التب من الداخل فبالتالي
+
+553
+00:39:56,570 --> 00:40:02,570
+صارت التب إشمالها مبللة، طيب فلمّا أجي أنقل volume
+
+554
+00:40:03,230 --> 00:40:06,390
+و أاخد ال volume و أنقله هيك بكون أنا عم باخد
+
+555
+00:40:06,390 --> 00:40:10,490
+volume بشكل أدق ليش؟ لأنه لو .. لو أنا ماعملت ال
+
+556
+00:40:10,490 --> 00:40:14,490
+pre-rinsing هذه طبعا لو ماعملت pre-rinsing أخدت
+
+557
+00:40:14,490 --> 00:40:18,350
+volume و روحت فضيته في جزء من السائل اللي أنا
+
+558
+00:40:18,350 --> 00:40:24,370
+أخدته بيضل ملتصق بجدران التب مبلل لإلها وهذا جزء
+
+559
+00:40:24,370 --> 00:40:29,190
+يعني في نسبة فقد لل volume في نسبة errorطبعا لكن
+
+560
+00:40:29,190 --> 00:40:34,490
+لما انا استخدم tip already مبللة يعني tip انا سحبت
+
+561
+00:40:34,490 --> 00:40:36,970
+اول volume فيها و كبيته و بعدين سحبت ال volume
+
+562
+00:40:36,970 --> 00:40:40,290
+اللى بدي استخدمه ال volume الفعلي معناه انا بللت
+
+563
+00:40:40,290 --> 00:40:45,850
+ال tip من الداخل فبالتالي فبطل في عندي نسبة فقد من
+
+564
+00:40:45,850 --> 00:40:49,890
+الحجم و بطل في عندي نسبة ال error هذه معناه كلمة
+
+565
+00:40:49,890 --> 00:40:53,670
+pre-rinsing minimize اشمالها ال variances
+
+566
+00:40:53,670 --> 00:40:59,560
+الاختلافات في ال .. ال ..الحجوم طبعا when by
+
+567
+00:40:59,560 --> 00:41:03,080
+beating some solutions زي ال serum اللي هي العينات
+
+568
+00:41:03,080 --> 00:41:08,260
+يعني الشخ .. المرضى ال serum البلازما ال protein
+
+569
+00:41:08,260 --> 00:41:11,300
+containing solution المحليل اللي بتحتوي على
+
+570
+00:41:11,300 --> 00:41:14,420
+البروتينات ال organic solvents المضيبات العضوية
+
+571
+00:41:14,420 --> 00:41:19,700
+هذه طبعا في جزء منها بيكون حكينا انه هيصير في فيلم
+
+572
+00:41:19,700 --> 00:41:24,500
+هيفي جزء منها هيبطن هيلامس ال inside of the tipو
+
+573
+00:41:24,500 --> 00:41:29,200
+هذا حكينا اشماله error فبالتالي لما انا اعمل عملية
+
+574
+00:41:29,200 --> 00:41:33,540
+pre-rinsing فبالتالي انا بقلل ال error هذه من خلال
+
+575
+00:41:33,540 --> 00:41:39,120
+ان انا خلاص استخدمت already جزء بيبة مبللة جزء ..
+
+576
+00:41:39,120 --> 00:41:43,040
+بيبة عملنا لها pre-rinsing فبالتالي مش هيكون في
+
+577
+00:41:43,040 --> 00:41:47,400
+عندي فقد في ال volume و مش هيكون في عندي نسبة
+
+578
+00:41:47,400 --> 00:41:51,780
+errorطيب when by beating concentrated acid or
+
+579
+00:41:51,780 --> 00:41:56,100
+highly corrosive solution such as halite salts
+
+580
+00:41:56,100 --> 00:42:01,540
+it's recommended to that by bit be disassembled
+
+581
+00:42:01,540 --> 00:42:07,680
+after used to inspect the shaft و المكونات تبعتها،
+
+582
+00:42:07,680 --> 00:42:11,800
+هلأ بيحكيلك أنه لما بنستخدم بعض المحاليل اللي هي
+
+583
+00:42:11,800 --> 00:42:18,100
+corrosive تمامحارقة او يعني مؤدية وبيتطير منها
+
+584
+00:42:18,100 --> 00:42:24,160
+ابخرة او يعني فيه بيتصعد منها قترانات او ابخرة او
+
+585
+00:42:24,160 --> 00:42:28,960
+رذاذ زي ال .. ال .. الهاليد سالتز هاي فيفضل ان انا
+
+586
+00:42:28,960 --> 00:42:32,620
+افك البعيبة و انظفها من الداخل و ارجع اركبها مرتان
+
+587
+00:42:32,620 --> 00:42:38,940
+علشان اتأكد انها هي يعني ما رح تتعطل ما رح تخرب ال
+
+588
+00:42:38,940 --> 00:42:44,050
+air bubbleطبعا يعني ان في عندي تقطع في السائل اللي
+
+589
+00:42:44,050 --> 00:42:47,170
+انا عم باخده في هنا فيه قطارات في فيه .. فيه هوا
+
+590
+00:42:47,170 --> 00:42:52,670
+تمام؟طب هذا ال .. ال .. الهواء إيش سببه؟ سببه أول
+
+591
+00:42:52,670 --> 00:42:55,970
+حاجة إما إن أنا رفعت إيدي عن ال plunger بشكل سريع
+
+592
+00:42:55,970 --> 00:43:00,010
+أو كانت ال tip مش مغمورة كويس في ال solution سحبت
+
+593
+00:43:00,010 --> 00:43:03,550
+من عن السطح فبالتالي بما إن سحبت عن السطح معناها
+
+594
+00:43:03,550 --> 00:43:10,810
+صار في عرض لإنه يدخل air bubble تمام ممكن يكون إنه
+
+595
+00:43:11,460 --> 00:43:19,940
+سببها ال .. ال .. يعني السائل نفسه يكون يعني فومي
+
+596
+00:43:19,940 --> 00:43:26,480
+بي .. بيكون رغوة فبالتالي انا ما اتبعت التعليمات ل
+
+597
+00:43:26,480 --> 00:43:31,360
+.. ل .. ل .. ال by betting من فومي reagent
+
+598
+00:43:31,360 --> 00:43:36,240
+فبالتالي ممكن تظهر عندى air bubble طبعا ال .. ال
+
+599
+00:43:36,240 --> 00:43:40,190
+.. ال air bubble إذا ظهرت طبعاطبعا أول حاجة إذا
+
+600
+00:43:40,190 --> 00:43:43,910
+ظهرت في .. في ال by bit بفضل solution و برجع باخد
+
+601
+00:43:43,910 --> 00:43:47,490
+مرة تانية، ضالت موجودة معناها بيده أتخلص من التب و
+
+602
+00:43:47,490 --> 00:43:51,090
+أركب تب جديدة و أاخد volume مع ال .. التزام
+
+603
+00:43:51,090 --> 00:43:56,410
+بالتعليمات حتى أتجنب من ظهور air bubble، ليش؟ لإن
+
+604
+00:43:56,410 --> 00:43:59,150
+ال air bubble طبعا هذه محتلة جزء من الحجم اللي
+
+605
+00:43:59,150 --> 00:44:03,580
+موجود يعني هذا المفروض كله يكون سائلطبعا فوجود air
+
+606
+00:44:03,580 --> 00:44:06,860
+bubble وجود تقطع في السائل يعني فيه هوى فبالتالي
+
+607
+00:44:06,860 --> 00:44:09,900
+الهوى محتل حجم اذا انا مش ماخدة الحجم المناسب
+
+608
+00:44:09,900 --> 00:44:14,360
+ماخدة اقل منه اذا انا مش accurate ومش precise طبعا
+
+609
+00:44:14,360 --> 00:44:19,420
+هذا بالنسبة ل ال bybit طيب خلينا بس قبل ما نشوف ال
+
+610
+00:44:19,420 --> 00:44:23,580
+calibration ل ال bybit نتابع مع بعض نشوف هذا
+
+611
+00:44:23,580 --> 00:44:30,040
+الفيديو بيعلمناكيف انا بدي استخدم ال .. ال by bet
+
+612
+00:44:30,040 --> 00:44:34,000
+و هنرجع نحكي نفس التعليمات او نفس المعلومات اللي
+
+613
+00:44:34,000 --> 00:44:37,560
+انا ذكرتهم باستخدام ال by bet هاي زي ما انتوا
+
+614
+00:44:37,560 --> 00:44:42,340
+شايفين هنا خليني بس ارجع شوية هنا تمام هاي ال by
+
+615
+00:44:42,340 --> 00:44:48,640
+bet نفس المكونات زي ما انتوا شايفين هنا نفس
+
+616
+00:44:48,640 --> 00:44:52,400
+المكونات لحظة خليني ارجع
+
+617
+00:44:55,930 --> 00:44:59,770
+خلص من هاي، هاي ال pipe هاي البلانجر تمام و الرد
+
+618
+00:44:59,770 --> 00:45:03,090
+هذا اللي بيلتف يمين و يسار تمام عكس حقارب الساعة و
+
+619
+00:45:03,090 --> 00:45:06,470
+باتجاه حقارب الساعة باتجاهي او بعيد عني حسب ما انا
+
+620
+00:45:06,470 --> 00:45:10,930
+هعلمكم ان شاء الله و هاي ال .. ال .. ال screen
+
+621
+00:45:10,930 --> 00:45:16,350
+تمام هذا ال .. ال .. ال ejector طبع التب على الجهة
+
+622
+00:45:16,350 --> 00:45:21,470
+التانية في هنا ال adapter هذا ال handle طبع ال ..
+
+623
+00:45:23,100 --> 00:45:27,640
+البيبت وهنا الموجود فيه ال adapter تبع التب تمام
+
+624
+00:45:27,640 --> 00:45:33,970
+ومهي المكونات كلها موجودة طيب خلينا نشوفكيف بيتم
+
+625
+00:45:33,970 --> 00:45:38,030
+تحويل حجم يعني من خلال .. يعني إما ب .. في بيبتات
+
+626
+00:45:38,030 --> 00:45:42,250
+بلف ال .. ال .. بلانجر نفسه بكون بحاركه، في بيبتات
+
+627
+00:45:42,250 --> 00:45:48,030
+بيكون لأ، في بيكون زي منظم موجود لأسفل منها و من
+
+628
+00:45:48,030 --> 00:45:51,970
+خلاله أنا بتحكم في الحجم، إذا بلفه علشان أتحكم
+
+629
+00:45:51,970 --> 00:45:56,840
+بالحجم اللي موجود هنا، شايفين؟ ها يعني تمام؟زي ما
+
+630
+00:45:56,840 --> 00:46:01,760
+انتوا شايفين بحرك بحيث ان انا اختار الحجم اللى بدى
+
+631
+00:46:01,760 --> 00:46:05,240
+طبعا ال by bit هى مكتوب هنا بتاخد من مية لألف هينا
+
+632
+00:46:05,240 --> 00:46:10,240
+ضبطناها على الف و بدى بعد هيك اروح اركبلها التب
+
+633
+00:46:10,240 --> 00:46:14,440
+الخاصة فيها طبعا لازم تكون تب مناسبة لإلها شايفين
+
+634
+00:46:14,440 --> 00:46:17,940
+التبات طبعا disposable يا بنات ال by bit التب اللى
+
+635
+00:46:17,940 --> 00:46:21,720
+بستخدمها برجعهاش مكانها بتخلص منها على طول هى التب
+
+636
+00:46:21,720 --> 00:46:29,190
+تمام هنا بيكون في هذه التب بيكون عندىsorry في هذه
+
+637
+00:46:29,190 --> 00:46:31,890
+ال tip بيكون في عندى الحجم اللى انا بدى انجله هاي
+
+638
+00:46:31,890 --> 00:46:35,830
+البلانجة طبعا انا لما اجى اكبس عليه بيفي عندى
+
+639
+00:46:35,830 --> 00:46:38,850
+first stop هاي first stop شايفين هادى اول
+
+640
+00:46:38,850 --> 00:46:42,190
+resistant بتقابلني هاي في عندى اذا هادى ال first
+
+641
+00:46:42,190 --> 00:46:47,900
+stop شايفين هنامجرد هي ضغطت فاتت عمودي في داخل
+
+642
+00:46:47,900 --> 00:46:52,680
+السائل طبعا خلينا ال tip يعني تكون مغمورة داخل
+
+643
+00:46:52,680 --> 00:46:57,400
+السائل و بنبدأ نرفع شوي شوي بالتدريج ايدنا عن
+
+644
+00:46:57,400 --> 00:47:01,540
+البلانجر ايش اللي هيصير بالظبط هيصير ان دخول
+
+645
+00:47:01,540 --> 00:47:05,220
+السائل داخل ال tip و هيك انا بكون أخدت الحجم اللي
+
+646
+00:47:05,220 --> 00:47:09,020
+موجود على ال screen طبعا لحظة انها ضغطت ضغطة واحدة
+
+647
+00:47:09,020 --> 00:47:13,660
+يعني هي ايش بتعملبتكون .. بتكون عم تعمل forward by
+
+648
+00:47:13,660 --> 00:47:17,900
+betting طبعا فهذا ال by bet او هذا ال volume بروح
+
+649
+00:47:17,900 --> 00:47:21,100
+باخده في container و بفضيه من خلال ضغطة أولى او
+
+650
+00:47:21,100 --> 00:47:24,140
+ضغطة تانية طبعا هاي شايفين الضغطة التانية هذه هي
+
+651
+00:47:24,140 --> 00:47:27,420
+الضغطة التانية طبعا هاي .. طبعا اذا انا .. انا هاي
+
+652
+00:47:27,420 --> 00:47:32,120
+هنا الضغطة بانقل ب .. باخد ال volume و بنقله لهذا
+
+653
+00:47:32,120 --> 00:47:41,050
+بفوت عمودي و لازم اتبع كل التعليمات طبعالو رجع
+
+654
+00:47:41,050 --> 00:47:48,310
+نركز عليها مرة تانية نشوف
+
+655
+00:47:48,310 --> 00:47:53,490
+هنا هى
+
+656
+00:47:53,490 --> 00:47:57,830
+هى هنا شايفين هادى الضغط
+
+657
+00:48:02,970 --> 00:48:07,490
+هي ضغطة وهي الضغطة التانية شايفين؟ هى معانا ضغطة
+
+658
+00:48:07,490 --> 00:48:13,530
+وضغطتين طبعا؟ طيب و هيك بيتم نقل حجوم بالشكل هذا
+
+659
+00:48:13,530 --> 00:48:17,800
+من الى طبعا؟ من الىال container الأصلي ل ال
+
+660
+00:48:17,800 --> 00:48:20,380
+container اللى بدى أشتغل فيه بنقل حجم اللى بديه
+
+661
+00:48:20,380 --> 00:48:25,340
+علشان أنا بكون بدى أجري تجربة معينة تمام لما باجى
+
+662
+00:48:25,340 --> 00:48:29,620
+بفضى لازم أنا أضغط على البلانجر بحيث أنه يفضى طبعا
+
+663
+00:48:29,620 --> 00:48:33,200
+البلانجر بضغط عليه بسحب بضغط عليه مرتان يدفضى و
+
+664
+00:48:33,200 --> 00:48:38,680
+هكذا يفضل يا بنات لما أنا أجى أسحب طبعا اللى أنتوا
+
+665
+00:48:38,680 --> 00:48:47,870
+شايفين هنا هي ال ..هنا شايفين كيف انا بفوت ضغطة
+
+666
+00:48:47,870 --> 00:48:51,790
+تمام لو
+
+667
+00:48:51,790 --> 00:48:52,390
+نرجع
+
+668
+00:48:55,290 --> 00:48:59,850
+تمام هى هنا فضة ال volume هى هنا ضغطة هى ضغطتين
+
+669
+00:48:59,850 --> 00:49:03,690
+تمام تعلمنا الضغطة و الضغطتين و هى هنا شايفين كده
+
+670
+00:49:03,690 --> 00:49:08,430
+أنا بفوت ضغطة و بعدين ب .. بسحب ال .. ب .. بترك
+
+671
+00:49:08,430 --> 00:49:12,630
+إيدي شوية شوية عن البلانجر مابنفع أنا أفوت مش ضغطة
+
+672
+00:49:12,630 --> 00:49:16,250
+و أضغط جوا ال solution ليش؟ لإنه ساعتها أنا بكون
+
+673
+00:49:16,250 --> 00:49:18,890
+بدخل هوا جوا ال solution إذا هو من النوع الفومي
+
+674
+00:49:18,890 --> 00:49:21,890
+اللى بيكون رغوة هيصير فيه رغوات و يصير في عندى air
+
+675
+00:49:21,890 --> 00:49:26,950
+bubble و مشاكل في نقل الحجومطبعا طيب هلأ انا بعد
+
+676
+00:49:26,950 --> 00:49:29,730
+ما انا فضيت ال volume ضغطت على البلانجر و فضيت ال
+
+677
+00:49:29,730 --> 00:49:32,350
+volume اش بده اعمل بده اضغط على ال ejector عشان
+
+678
+00:49:32,350 --> 00:49:36,370
+اتخلص من التب شايفين مجرد ما اضغط عليها ال .. ال
+
+679
+00:49:36,370 --> 00:49:40,890
+.. التب بتفصل عن ال bibert و بت .. بتم التخلص منها
+
+680
+00:49:40,890 --> 00:49:46,460
+طبعاهذا بالنسباله ال .. ال .. ال bybite اذا اول
+
+681
+00:49:46,460 --> 00:49:52,560
+حاجة بنحدد الحجم ب .. أركب ال tip بعمل rinsing pre
+
+682
+00:49:52,560 --> 00:49:58,200
+-rinsing ببللها تمام و بعدين باخد الحجم اللي بدياه
+
+683
+00:49:58,200 --> 00:50:01,960
+سواء كان باخده بطريقة ال .. ال .. ال forward
+
+684
+00:50:01,960 --> 00:50:06,000
+bybiting او ال reverse bybiting حسب اللي انا الحجم
+
+685
+00:50:06,000 --> 00:50:09,930
+اللي ال .. ال .. او السائل اللي انا بتعامل معاهو
+
+686
+00:50:09,930 --> 00:50:15,030
+بانقله من و إلى بعد هيك بتخلص من التب بهذا الشكل
+
+687
+00:50:15,030 --> 00:50:21,550
+تمام؟ ان شاء الله هيكون شغلنا على ال .. ال bybit و
+
+688
+00:50:21,550 --> 00:50:24,630
+طبعا معظم .. كل الفحوصات بتنعمل باستخدام ال bybit
+
+689
+00:50:24,630 --> 00:50:28,630
+انا بدي اضيف حجم من ال reagent و حجم من العينة حجم
+
+690
+00:50:28,630 --> 00:50:31,510
+من ال standard و حجم من ال reagent كل شغلي
+
+691
+00:50:31,510 --> 00:50:33,710
+باستخدام ال bybit اذا هذه صديقتنا هذه اللي
+
+692
+00:50:33,710 --> 00:50:36,390
+هترافقنا من شاء الله من سنة تانية لسنة رابعة لجهة
+
+693
+00:50:36,390 --> 00:50:39,950
+الأداء فبالتالي لازم نكون دقيقين في استخدامهاو
+
+694
+00:50:39,950 --> 00:50:42,690
+عشان .. عشان نكون دقيقين باستخدامها و تكون ال vibe
+
+695
+00:50:42,690 --> 00:50:46,370
+تهدي احنا بنعمللها صيانة و بنضمن دقتها باستمرار
+
+696
+00:50:46,370 --> 00:50:49,530
+لازم انه انا اعمللها calibration اش يعني
+
+697
+00:50:49,530 --> 00:50:52,890
+calibration يعني اعادة ضبط وتهيئة علشان اتأكد من
+
+698
+00:50:52,890 --> 00:50:57,070
+شغلها ان هي عم تاخدلي شغل دقيق طبعا كيف انا بدي
+
+699
+00:50:57,070 --> 00:51:01,660
+اخد ال .. ال .. اكيد اعمللها calibration؟بدي
+
+700
+00:51:01,660 --> 00:51:06,200
+استخدمها يعني او اظبط شغلها او اتأكد من انها عم
+
+701
+00:51:06,200 --> 00:51:10,820
+تاخد volumes بشكل دقيق باستخدام الحجوم الماء ال
+
+702
+00:51:10,820 --> 00:51:14,960
+distilled water تمام؟ هنا بيحكيلي انه تحت درجة
+
+703
+00:51:14,960 --> 00:51:21,200
+الحرارة و الضغط الثابت الكثافة الماء أيضا ثابتة
+
+704
+00:51:21,200 --> 00:51:26,860
+فالحجم من الماء تمام ممكن انا يعني one ml من الميه
+
+705
+00:51:26,860 --> 00:51:31,820
+بيعادل one gramمن الميه تمام ال .. ال one ml ال
+
+706
+00:51:31,820 --> 00:51:36,360
+volume one ml لو أنا جيت وزنته تحت درجة حرارة وضغط
+
+707
+00:51:36,360 --> 00:51:40,400
+ثابت وكثافة ثابتة للماء فهي .. ال .. ال one ml
+
+708
+00:51:40,400 --> 00:51:46,710
+هيعطيني one gram من ال water تمام؟ طيب فبالتاليال
+
+709
+00:51:46,710 --> 00:51:51,330
+.. ال calibration لل بايبت لازم انا اكون بس يعني
+
+710
+00:51:51,330 --> 00:51:54,390
+بدي اعرف ال volume اللي عم تاخده حجم الماء اللي
+
+711
+00:51:54,390 --> 00:51:59,890
+بتاخده و هل هو accurate او precise و طبعا هذا
+
+712
+00:51:59,890 --> 00:52:06,910
+بيتأثر بدرجة حرارة الغرفة و ايضا الضغط الجوي و
+
+713
+00:52:06,910 --> 00:52:10,190
+الرطوبة نسبة الرطوبة اللي موجودة في الوسط او في
+
+714
+00:52:10,190 --> 00:52:14,320
+البيئة المحيطة لإجراء التجربةعشان هيك انا بستخدم
+
+715
+00:52:14,320 --> 00:52:17,980
+factor او ثابت اسمه z factor في الحسابات طبعتي
+
+716
+00:52:17,980 --> 00:52:23,340
+هنحكي عنه كمان شوية هذا ال z factor فالفكرة طبعتي
+
+717
+00:52:23,340 --> 00:52:27,660
+انه انا بدي اخد one ml من ال distilled water يعني
+
+718
+00:52:27,660 --> 00:52:30,980
+بدي اجيب ال by bit بدي اعمللها setting بدي ا ..
+
+719
+00:52:30,980 --> 00:52:35,700
+اضب .. اضبطها على one ml اركب tip اخد اعمل ال pre
+
+720
+00:52:35,700 --> 00:52:39,920
+-rinsing ابلل ال tip من .. و اتخلص من ال solution
+
+721
+00:52:39,920 --> 00:52:44,350
+اللي أخدته في المرة الأولىبعد هيك إيش بدي أعمل؟
+
+722
+00:52:44,350 --> 00:52:50,110
+بدي أخد one ml من distilled water و أحطه في cup ال
+
+723
+00:52:50,110 --> 00:52:53,990
+cup هذا بدي أكون مسفر عليه في الميزان بدي أسفر
+
+724
+00:52:53,990 --> 00:52:57,590
+عليه و أحط ال one ml و أشوف الوزنة تبعتي الوزنة
+
+725
+00:52:57,590 --> 00:53:00,090
+تبعتي هتكون إذا هو مسفر على ال cup فالوزنة اللي
+
+726
+00:53:00,090 --> 00:53:05,050
+هتطلع معايا هي وزنت ال one ml المفروض تكون مقاربة
+
+727
+00:53:05,050 --> 00:53:08,470
+ل one gram تمام؟ المفروض انها تكون مقاربة ل one
+
+728
+00:53:08,470 --> 00:53:13,310
+gram او .. او يعني exactly one gramطبعا فعشان ان
+
+729
+00:53:13,310 --> 00:53:16,130
+احنا عندنا درجة الحرارة و الدغط الجوي و ال
+
+730
+00:53:16,130 --> 00:53:21,070
+humidity متغيرة فانا بروح بدرب الوزن هذه تبعتي بال
+
+731
+00:53:21,070 --> 00:53:25,390
+Z factor و في جدول في اخر ال .. ال .. ال slide
+
+732
+00:53:25,390 --> 00:53:29,590
+بعلمني كيف انا اختار ال Z factor المناسب اللي بدي
+
+733
+00:53:29,590 --> 00:53:36,880
+ادرب في القراءات تبعتي طبعا فإذا ال ..الحجم هي هنا
+
+734
+00:53:36,880 --> 00:53:41,300
+مثلا إيش بلزمني sorry إيش بلزمني لأ التجربة تبعتي؟
+
+735
+00:53:41,300 --> 00:53:46,100
+بلزمني by bit و tip بلزمني beaker أو cup لحتى أحطه
+
+736
+00:53:46,100 --> 00:53:49,220
+على الميزان و أصفر عليه تمام؟ بلزمني distilled
+
+737
+00:53:49,220 --> 00:53:54,260
+water بلزمني أقيس درجة الحرارة و ميزان و أقيس ال
+
+738
+00:53:54,260 --> 00:54:00,850
+pressure الدغط عشان أطلع z factorأول إشي بدي أقيس
+
+739
+00:54:00,850 --> 00:54:07,510
+درجة حرارة الماء و أسجلها بعد هيك باستخدام beaker
+
+740
+00:54:07,510 --> 00:54:10,950
+طبعا انه انا بدي أحطه على ال .. طبعا مش راح أقرأ
+
+741
+00:54:10,950 --> 00:54:18,900
+كل الخطوات هايراح اختصرها باستخدام ال beaker او ال
+
+742
+00:54:18,900 --> 00:54:23,080
+cup راح احطه على ال analytical balance و راح هصفر
+
+743
+00:54:23,080 --> 00:54:27,080
+عليه طبعا دي خلي ا .. ا .. اخلي الميزان يعتبر ان
+
+744
+00:54:27,080 --> 00:54:32,040
+وزن ال cup هذا zero طبعابعد هيك بدي أعمل pre ..
+
+745
+00:54:32,040 --> 00:54:35,520
+بدي أركب تب للبيبط و بعد ما أكون أنا حددت الحجم
+
+746
+00:54:35,520 --> 00:54:40,840
+المناسب لإلها اللي هو one ml يعني الف microliter و
+
+747
+00:54:40,840 --> 00:54:44,940
+بعد هيك بدي أعمل pre-rinsing عرفنا إيش يعني pre
+
+748
+00:54:44,940 --> 00:54:49,040
+-rinsing يعني أنا بدي أسحب أسبيرات and spins يعني
+
+749
+00:54:49,040 --> 00:54:53,640
+بدي أسحب و أتخلص من الحجم على أساس ممكن أعيد هذه
+
+750
+00:54:53,640 --> 00:54:56,860
+الخطوة تلت مرات على أساس أتأكد أنه ال .. ال ..
+
+751
+00:54:56,860 --> 00:54:59,580
+التب صارت مبللة من الداخل يعني مش هيكون في عندى لا
+
+752
+00:54:59,580 --> 00:55:05,320
+.. لا فقطولا error في ال volume تمامو بعد هيك إيش
+
+753
+00:55:05,320 --> 00:55:09,880
+بدي أعمل؟ بدي أضغط على ال .. ال .. ال .. ال .. ال
+
+754
+00:55:09,880 --> 00:55:11,120
+.. ال .. ال .. ال .. ال .. ال .. ال .. ال .. ال ..
+
+755
+00:55:11,120 --> 00:55:11,380
+ال .. ال .. ال .. ال .. ال .. ال .. ال .. ال .. ال
+
+756
+00:55:11,380 --> 00:55:11,580
+.. ال .. ال .. ال .. ال .. ال .. ال .. ال .. ال ..
+
+757
+00:55:11,580 --> 00:55:13,040
+ال .. ال .. ال .. ال .. ال .. ال .. ال .. ال .. ال
+
+758
+00:55:13,040 --> 00:55:13,160
+.. ال .. ال .. ال .. ال .. ال .. ال .. ال .. ال ..
+
+759
+00:55:13,160 --> 00:55:13,660
+ال .. ال .. ال .. ال .. ال .. ال .. ال .. ال .. ال
+
+760
+00:55:13,660 --> 00:55:13,660
+.. ال .. ال .. ال .. ال .. ال .. ال .. ال .. ال ..
+
+761
+00:55:13,660 --> 00:55:14,400
+ال .. ال .. ال .. ال .. ال .. ال .. ال .. ال .. ال
+
+762
+00:55:14,400 --> 00:55:21,420
+.. ال .. ال ..
+
+763
+00:55:21,420 --> 00:55:21,420
+ال .. ال .. ال .. ال .. ال .. ال .. ال .. ال .. ال
+
+764
+00:55:21,420 --> 00:55:21,420
+.. ال .. ال .. ال .. ال .. ال .. ال .. ال .. ال ..
+
+765
+00:55:21,420 --> 00:55:21,440
+ال .. ال .. ال .. ال .. ال .. ال .. ال .. ال .. ال
+
+766
+00:55:21,440 --> 00:55:25,140
+.. ال ..
+
+767
+00:55:34,190 --> 00:55:40,910
+عشان السائل يفوت داخل التب أيضا بشكل متأني يعني
+
+768
+00:55:40,910 --> 00:55:44,210
+انه مايصير فيه اندفاع لا solution و هي air bubble
+
+769
+00:55:44,210 --> 00:55:48,260
+و المشاكل اللي ممكن تحدث في البيت انجلتمام؟ هيك
+
+770
+00:55:48,260 --> 00:55:51,980
+أنا أخدت الحجم اللي بدياه بروح بفضيه في ال beaker
+
+771
+00:55:51,980 --> 00:55:55,340
+أو ال cup اللي موجود على الميزان و باخد القراءة
+
+772
+00:55:55,340 --> 00:55:59,760
+تمام؟ بعد هيك ممكن أنا أرجع أصفر على ال cup و ال
+
+773
+00:55:59,760 --> 00:56:03,420
+.. ال volume اللي موجود فيه أو أن أنا أستخدم cup
+
+774
+00:56:03,420 --> 00:56:08,100
+جديد إيه للخيار؟بسفر و بعيد الخطوة مرة تانية باخد
+
+775
+00:56:08,100 --> 00:56:13,560
+one amal بالبيبت و بفضيه و باخد كمان وزنة و كمان
+
+776
+00:56:13,560 --> 00:56:17,340
+مرة تالتة باخد one amal و بفضيه في الميزان و باخد
+
+777
+00:56:17,340 --> 00:56:22,320
+وزنة تالتة فإذا صار في عندي تلت وزنات لتلت حجوم
+
+778
+00:56:22,320 --> 00:56:25,400
+تمام فإيش بدي أعمل في التلت وزنات هدول؟ بدي أجيب
+
+779
+00:56:25,400 --> 00:56:28,740
+ال average تبعهم يعني بدي أجيب مجموعهم على تلتة
+
+780
+00:56:29,100 --> 00:56:34,400
+تمام؟ ال .. هذه ال ..ش اسمه؟ ال .. يعني تكملت
+
+781
+00:56:34,400 --> 00:56:37,220
+التجربة اللي انا حكيتها يعني انه .. انه عيدي
+
+782
+00:56:37,220 --> 00:56:41,380
+الخطوات تلت مرات و جيبي القراءة هي هنا مثلا
+
+783
+00:56:41,380 --> 00:56:44,340
+بيحكيلي ممكن تعديها اربعتاشر مرات حسب يعني ممكن
+
+784
+00:56:44,340 --> 00:56:48,200
+تعديها تلت مرات اربع مرات خمس مرات المهم بالنهاية
+
+785
+00:56:48,200 --> 00:56:51,640
+الوزنات اللي تطلع معاكي بدك تاخديلها average ايش
+
+786
+00:56:51,640 --> 00:56:56,440
+يعني average؟ يعني مجموعها على عددهاو اطلع قراءة
+
+787
+00:56:56,440 --> 00:56:59,680
+واحدة القراءة واحدة هي هذه اللي بدك تروحي اتعاوضي
+
+788
+00:56:59,680 --> 00:57:02,940
+فيها بالقانون ال weight او ال average of weight
+
+789
+00:57:02,940 --> 00:57:06,180
+اللي طلع معاكي و بدك تضربيه في ال z factor اللي هو
+
+790
+00:57:06,180 --> 00:57:10,980
+معامل اللي هو الحرارة و الضغط تمام اللي موجود عندك
+
+791
+00:57:10,980 --> 00:57:14,980
+و بالنهاية بحسب ال volumeانا خدت ال bybit كانت
+
+792
+00:57:14,980 --> 00:57:18,940
+بتحكيلي ان انا باخد one ml على الشاشة تبع ال bybit
+
+793
+00:57:18,940 --> 00:57:22,840
+مكتوب one ml فالمفروض ان هذا الكلام ال wet في ال
+
+794
+00:57:22,840 --> 00:57:27,020
+zip factor يكون one ml ايضا يعني او مقارب ل one ml
+
+795
+00:57:27,020 --> 00:57:34,050
+فممكن اناأطبق قوانين التحليلية يعني هي ان انا بجيب
+
+796
+00:57:34,050 --> 00:57:39,050
+ال average لقراءات الوزنة اللي هي مجموع القراءات
+
+797
+00:57:39,050 --> 00:57:43,170
+على عددها تمام ممكن اطبق ايضا احسب ال standard
+
+798
+00:57:43,170 --> 00:57:47,870
+deviation اجيب ال accuracy يعني القيمة الوزنة اللي
+
+799
+00:57:47,870 --> 00:57:50,610
+طلعت معايا و اللي المفروض تطلع مثلا طلعت معايا
+
+800
+00:57:50,610 --> 00:57:54,690
+point 999 الوزنة و المفروض انها تطلع one milligram
+
+801
+00:57:54,690 --> 00:57:58,280
+بحسبهابس شوف .. شوف نسبة ال accuracy تبعت القيمة
+
+802
+00:57:58,280 --> 00:58:02,100
+تبعتي و ال coefficient of variation اللي هي ..
+
+803
+00:58:02,100 --> 00:58:04,560
+اللي هي عبارة عن ال standard deviation على ال mean
+
+804
+00:58:04,560 --> 00:58:11,920
+ضارب 100% لازم ال accuracy هذه تطلع من 99 ل 101% و
+
+805
+00:58:11,920 --> 00:58:16,470
+لازمال coefficient of variation يكون اقل من واحد
+
+806
+00:58:16,470 --> 00:58:19,690
+في المية هذا يكون اقل من واحد في المية عشان اقول
+
+807
+00:58:19,690 --> 00:58:22,290
+ان ال by bit هذه accurate و precise و بتعطيني
+
+808
+00:58:22,290 --> 00:58:25,570
+volumes دقيقة و الحجم اللي انا باخده هو نفسه اللي
+
+809
+00:58:25,570 --> 00:58:28,990
+موجود على الشاشة و شغلها مية لمية اذا انا لما بدي
+
+810
+00:58:28,990 --> 00:58:31,930
+اجري افحصات و بدي انقل volume باستخدام هذه ال by
+
+811
+00:58:31,930 --> 00:58:36,740
+bit اذا انا عم بشتغل بشكل صحيح و بشكل دقيقطبعا هذا
+
+812
+00:58:36,740 --> 00:58:40,740
+الجدول اللى انا من خلاله بختار مثلا ال .. ال z
+
+813
+00:58:40,740 --> 00:58:45,180
+value او اللى هو معامل ال .. الحرارة و الضغط طبعا
+
+814
+00:58:45,180 --> 00:58:47,980
+ال z value مثلا انا بشوف ايش الضغط اللى موجود عندى
+
+815
+00:58:47,980 --> 00:58:50,040
+حسب المنطقة اللى انا .. اللى انا فيها لان افرض
+
+816
+00:58:50,040 --> 00:58:54,620
+مثلا ان انا موجودة في منطقة ضغطها الف و ست .. سبعة
+
+817
+00:58:54,620 --> 00:58:59,420
+و ستين ملي بار طبعاو درجة الحرارة عندي خمسة و
+
+818
+00:58:59,420 --> 00:59:04,240
+عشرين فبروح هنا هي خمسة و عشرين بمشي لعند مين لعند
+
+819
+00:59:04,240 --> 00:59:08,100
+ال .. ال .. ال .. المنطقة اللي هي ال .. اللي فيها
+
+820
+00:59:08,100 --> 00:59:11,600
+الضغط تبعي تمام؟ اللي فيها الضغط تبعي هنا هيك هي
+
+821
+00:59:11,600 --> 00:59:15,480
+هذه القيمة تقاطع درجة الحرارة اللي بديها مع الضغط
+
+822
+00:59:15,480 --> 00:59:18,480
+اللي .. اللي بديها هي هذه قيمة ال Z factor اللي
+
+823
+00:59:18,480 --> 00:59:22,300
+بروح ب .. ب .. أطبق فيهاتمام هي هذه قيمة ال z
+
+824
+00:59:22,300 --> 00:59:26,060
+factor اللي بروح بطبق فيها و بحسب حسابات تبعتي زي
+
+825
+00:59:26,060 --> 00:59:29,820
+ما حكينا لو انا عديت التجربة مرة مرتين تلاتة اربع
+
+826
+00:59:29,820 --> 00:59:34,900
+عشرة و حصلت على مثلا عشر قيم باخد ال average تبعهم
+
+827
+00:59:34,900 --> 00:59:39,100
+بدربهم بال z factor بحصل على ال volume و بطبق في
+
+828
+00:59:39,100 --> 00:59:43,180
+القوانين تبعتي القيمة اللي انا جستها على القيمة
+
+829
+00:59:43,180 --> 00:59:46,570
+اللي المفروض تطلعضرب 100% بحسب ال accuracy بحسب ال
+
+830
+00:59:46,570 --> 00:59:50,450
+coefficient of variation بشوف نسبة دقة و صحة ال ..
+
+831
+00:59:50,450 --> 00:59:54,070
+ال vibe بتاعي و بحكم على شغلها و هل انها محتاجة
+
+832
+00:59:54,070 --> 01:00:00,690
+انها يتم تصليحها او انه لأ مناسبة و تستخدم يعني
+
+833
+01:00:00,690 --> 01:00:05,350
+موثوقة للاستخدام تمام؟بهيك بنكون خلصنا محاضرتنا
+
+834
+01:00:05,350 --> 01:00:10,030
+لليوم ان شاء الله تكون هيك الاشياء واضحة حولت ان
+
+835
+01:00:10,030 --> 01:00:15,350
+انا اورجيكم صور اورجيكم فيديو لكيف ال by بتاعي كنت
+
+836
+01:00:15,350 --> 01:00:18,810
+نستخدمها و تعليمات اللي بنتابعها أثناء ال by
+
+837
+01:00:18,810 --> 01:00:25,490
+bettingلعسى انه يعني تكون الاشي مش مبهم لقلكم لإنه
+
+838
+01:00:25,490 --> 01:00:29,810
+يعني احنا مقدرين انه بداية التخصص لازم تكون ..
+
+839
+01:00:29,810 --> 01:00:35,650
+تكون يعني كل إشي سهل ومستثار لحتى تتمكنوا من الفهم
+
+840
+01:00:35,650 --> 01:00:40,430
+المعلومة وإدراكها يعطيكم ألف عافية وشكرا لكم
+
diff --git a/PL9fwy3NUQKwY9jl8xw-bWkVZkWcGGyspd/ZZ8KPmQPnAc.srt b/PL9fwy3NUQKwY9jl8xw-bWkVZkWcGGyspd/ZZ8KPmQPnAc.srt
new file mode 100644
index 0000000000000000000000000000000000000000..39f1af29b92475e5998bd8958e4ccfe873fec653
--- /dev/null
+++ b/PL9fwy3NUQKwY9jl8xw-bWkVZkWcGGyspd/ZZ8KPmQPnAc.srt
@@ -0,0 +1,2320 @@
+1
+00:00:05,360 --> 00:00:08,860
+السلام عليكم ورحمة الله وبركاته طالبات العزيزات
+
+2
+00:00:08,860 --> 00:00:11,340
+اليوم إن شاء الله راح نشرح hemoglobin
+
+3
+00:00:11,340 --> 00:00:14,260
+concentration determination lecture راح نكون مع
+
+4
+00:00:14,260 --> 00:00:17,920
+بعض في محاضرة بعنوان hemoglobin concentration
+
+5
+00:00:17,920 --> 00:00:22,940
+determination اللي هو يعني معرفة أو قياس تركيز ال
+
+6
+00:00:22,940 --> 00:00:27,140
+hemoglobin و راح نتعرف كيف احنا ممكن نقيس نسبة ال
+
+7
+00:00:27,140 --> 00:00:31,480
+hemoglobin في الدم وبطريقة أو بتطبيق ال Beer
+
+8
+00:00:31,480 --> 00:00:37,970
+Lambert law hemoglobin HP. هذا ال HP هو عبارة عن the
+
+9
+00:00:37,970 --> 00:00:43,070
+standard abbreviation for hemoglobin هو الاختصار
+
+10
+00:00:43,070 --> 00:00:47,310
+اللي متعارف عليه لبروتين ال hemoglobin سواء حكيت لك
+
+11
+00:00:47,310 --> 00:00:51,350
+hemoglobin أو كتبت لك HP فأنا بكون بقصد ال
+
+12
+00:00:51,350 --> 00:00:56,070
+hemoglobin. البروتين ال hemoglobin هذا هو عبارة عن
+
+13
+00:00:56,070 --> 00:01:01,430
+ال oxygen carrying pigment هو الناقل للأكسجين،
+
+14
+00:01:01,430 --> 00:01:05,510
+تمام؟ هو اللي بيحمل الأكسجين داخل خلايا ال RBCs و
+
+15
+00:01:05,510 --> 00:01:09,850
+هو ال pigment .. التركيبة ال .. ال hemoglobin
+
+16
+00:01:09,850 --> 00:01:15,130
+تعطيه لون أحمر، إذا هو اللي يكسب خلايا الدم
+
+17
+00:01:15,130 --> 00:01:19,590
+الحمراء لونها الأحمر، و من هنا جاءت تسمية ال RBCs
+
+18
+00:01:19,590 --> 00:01:24,030
+بال red blood cells، تمام؟ من لونها، من لون ال
+
+19
+00:01:24,030 --> 00:01:28,690
+hemoglobin فيها. الآن هذا ال hemoglobin هو ال  predominant
+
+20
+00:01:28,690 --> 00:01:32,710
+protein هو ال protein الأكثر شيوعا و
+
+21
+00:01:32,710 --> 00:01:38,370
+المحتل أكبر حجم من خلايا ال RBCs أكثر من 60% من
+
+22
+00:01:38,370 --> 00:01:44,950
+حجم ال RBCs هو عبارة عن hemoglobin تمام؟ هو عبارة
+
+23
+00:01:44,950 --> 00:01:48,690
+عن hemoglobin. Hemoglobin carries oxygen from
+
+24
+00:01:48,690 --> 00:01:53,580
+places of high oxygen pressure (lungs) to places of
+
+25
+00:01:53,580 --> 00:01:57,660
+low oxygen pressure such as tissues. ال hemoglobin
+
+26
+00:01:57,660 --> 00:02:03,240
+حكينا إيش وظيفته؟ هو عبارة عن ناقل للأكسجين،
+
+27
+00:02:03,240 --> 00:02:08,560
+إذا هو أثناء سريان الدم و يذهب إلى ال lungs،
+
+28
+00:02:08,560 --> 00:02:12,900
+للرئتين، في الرئتين موجود ضغط عالي من الأكسجين،
+
+29
+00:02:12,900 --> 00:02:15,920
+تمام؟ لأن الرئتين هي اللي بتستلم الأكسجين، نعمل
+
+30
+00:02:15,920 --> 00:02:20,100
+إحنا التنفس، شاهق، الأكسجين بيدخل إلى الرئتين، إذاً
+
+31
+00:02:20,100 --> 00:02:23,440
+الرئتين فيها high oxygen pressure ساعتها
+
+32
+00:02:23,440 --> 00:02:26,120
+ال hemoglobin لما يكون عند الرئتين أو يكون في
+
+33
+00:02:26,120 --> 00:02:31,720
+الرئتين إيش راح يعمل؟ راح يبدأ يحمل أكسجين، تمام؟
+
+34
+00:02:31,720 --> 00:02:36,560
+ال hemoglobin راح يحمل أكسجين في الرئتين، ولما يعمل
+
+35
+00:02:36,560 --> 00:02:41,180
+circulation، هذا ال hemoglobin المحمل بالأكسجين يصل
+
+36
+00:02:41,180 --> 00:02:46,100
+إلى tissue أو ال organs أعضاء الجسم أو أنسجة الجسم
+
+37
+00:02:46,100 --> 00:02:50,440
+اللي عندها low oxygen pressure، ما فيش عندها oxygen
+
+38
+00:02:50,440 --> 00:02:54,780
+كفاية، إيش بيروح بيعمل؟ بيبدأ يترك هذا الأكسجين اللي
+
+39
+00:02:54,780 --> 00:02:59,720
+حمله. إذا ال hemoglobin هي هيك خواص عمله في ال high
+
+40
+00:02:59,720 --> 00:03:06,710
+oxygen pressure بيبدأ يستلم يحمل oxygen، تمام؟ و في
+
+41
+00:03:06,710 --> 00:03:10,210
+ال low oxygen pressure عند ال tissue بيبدأ يترك
+
+42
+00:03:10,210 --> 00:03:14,530
+oxygen، عشان هيك بنحكي عنه ناقل، carrier، delivery
+
+43
+00:03:14,530 --> 00:03:21,250
+لل oxygen. Hemoglobin also returns CO2 from the
+
+44
+00:03:21,250 --> 00:03:25,890
+tissue to the lung. فالمقابل هذا ال hemoglobin بي
+
+45
+00:03:25,890 --> 00:03:31,290
+.. مقابل أنه بيعطي ال tissue أكسجين، هو يحمل ال
+
+46
+00:03:31,290 --> 00:03:36,330
+CO2 من ال tissue و بيرجعها لل lung علشان تخرج في
+
+47
+00:03:36,330 --> 00:03:41,630
+عملية الزفير. هذا ال hemoglobin molecule يتكون من
+
+48
+00:03:41,630 --> 00:03:49,150
+جزئين، شق بروتيني بنسميه globin، هو شق non-protein
+
+49
+00:03:49,150 --> 00:03:54,270
+بنسميه الهيم، تمام؟ عشان هيك هو اسمه hemoglobin
+
+50
+00:03:54,270 --> 00:03:59,970
+تمام؟ شق بروتيني ال globin، وشق non-protein اللي هو
+
+51
+00:03:59,970 --> 00:04:05,550
+الهيم. هذا الهيم عبارة عن أربع حلقات من الهيم، أربع
+
+52
+00:04:05,550 --> 00:04:11,370
+حلقات heme، كل واحدة منهم مرتبطة مع جزئ iron أو ذرة
+
+53
+00:04:11,370 --> 00:04:16,600
+iron. iron تمام؟ في ال ferrous state. إيش يعني في
+
+54
+00:04:16,600 --> 00:04:23,160
+ال ferrous state؟ يعني iron Fe +2 شحنتها. وهنا
+
+55
+00:04:23,160 --> 00:04:26,840
+حاكـي.. حاكيني one molecule of globin، جزء globin
+
+56
+00:04:26,840 --> 00:04:31,160
+معبر عن كل بروتين، يعني على كل الشكل البروتيني عبّر
+
+57
+00:04:31,160 --> 00:04:36,220
+عنه بإن هو ال globin part، تمام؟ ولكن هذا ال globin
+
+58
+00:04:36,220 --> 00:04:42,560
+part مكون من أربع سلاسل ببتيدية، four polypeptide
+
+59
+00:04:42,560 --> 00:04:48,380
+chains، تمام؟ اثنتين منهم من نوع alpha chain، واثنتين
+
+60
+00:04:48,380 --> 00:04:53,600
+من نوع beta chain. هذه السلاسل ال alpha chain
+
+61
+00:04:53,600 --> 00:04:57,840
+الواحدة منهم طولها 141 amino acid، و ال beta
+
+62
+00:04:57,840 --> 00:05:03,980
+الواحدة منهم 146 amino acid. تمام؟ طيب خلينا بس نشوف
+
+63
+00:05:03,980 --> 00:05:07,740
+مع بعض كيف هو تركيب ال hemoglobin. ال hemoglobin
+
+64
+00:05:07,740 --> 00:05:11,060
+مكون من ال globin part اللي هو ال protein part
+
+65
+00:05:11,060 --> 00:05:16,360
+الجزء ال protein منه، و ال heme part، الحلقات ال heme
+
+66
+00:05:16,360 --> 00:05:20,900
+الأربعة اللي هي non-protein part، مش .. تركيبها
+
+67
+00:05:20,900 --> 00:05:23,460
+مش protein، يعني ما فيش فيها peptide bond، ما فيش فيها
+
+68
+00:05:23,460 --> 00:05:29,030
+amino acids، يعني عشان نحكي عنها بروتينية. تمام؟ طيب
+
+69
+00:05:29,030 --> 00:05:35,090
+هذا ال globin part مكون من 4 سلاسل، اثنتين alpha
+
+70
+00:05:35,090 --> 00:05:40,310
+مقابل بعضهم البعض، و اثنتين beta، اثنتين alpha و اثنتين
+
+71
+00:05:40,310 --> 00:05:47,170
+beta بهذا الشكل. ال alpha الواحدة منهم طولها 141
+
+72
+00:05:47,170 --> 00:05:53,910
+amino acid، ال beta طولها 146 amino acid، وكل سلسلة
+
+73
+00:05:53,910 --> 00:05:58,170
+منها دول بتكون الواحدة منهم مرتبطة بحلقة heme
+
+74
+00:05:58,170 --> 00:06:04,790
+تمام؟ حلقة heme. حلقة ال heme هذه في مركزها موجود
+
+75
+00:06:04,790 --> 00:06:09,810
+ال iron تمام؟ بال ferrous state اللي هو iron Fe
+
+76
+00:06:09,810 --> 00:06:16,570
++2 بيكون طبعا هذا.. عند حلقة ال iron هاي أو
+
+77
+00:06:16,570 --> 00:06:20,800
+حلقة ال heme اللي في مركزها iron. هنا يرتبط ال
+
+78
+00:06:20,800 --> 00:06:25,260
+oxygen.. هنا يحمل ال oxygen على ال hemoglobin
+
+79
+00:06:25,260 --> 00:06:30,540
+تمام؟ و بالمقابل طبعا في ال lung يحمل ال oxygen و
+
+80
+00:06:30,540 --> 00:06:35,300
+يتم تركه في ال tissue و.. و يحمل بداله مين؟ CO2
+
+81
+00:06:35,300 --> 00:06:40,420
+عشان يرجع.. يرجعه إلى ال lung و يتم التخلص منه في
+
+82
+00:06:40,420 --> 00:06:46,870
+عملية الزفير. A heme group is a flat ring molecule
+
+83
+00:06:46,870 --> 00:06:52,110
+containing carbon, nitrogen and hydrogen and
+
+84
+00:06:52,110 --> 00:06:57,130
+oxygen. تمام؟ ال heme هي عبارة عن حلقة مسطحة من ال
+
+85
+00:06:57,130 --> 00:07:00,410
+hydrogen و ال carbon و ال.. و ال nitrogen، مرتبطة
+
+86
+00:07:00,410 --> 00:07:03,590
+مع بعضها البعض، و في وجود أيضا لل oxygen، و هي
+
+87
+00:07:03,590 --> 00:07:09,330
+توسطها موجود عندي Fe +2 في هذه molecule وين؟
+
+88
+00:07:09,330 --> 00:07:14,190
+في ال center، تمام؟ في ال center. Without the iron,
+
+89
+00:07:14,190 --> 00:07:18,070
+بدون ال iron،  نأخذ ال iron شوية، without the iron،
+
+90
+00:07:18,070 --> 00:07:23,130
+هذه الحلقة بنسميها porphyrin ring. مع ال iron بتصير
+
+91
+00:07:23,130 --> 00:07:30,750
+heme. Without iron, porphyrin; with iron, heme. تمام؟
+
+92
+00:07:30,750 --> 00:07:35,370
+فلازم إنه نعرف هذه الأساسيات عن تركيب ال
+
+93
+00:07:35,370 --> 00:07:41,030
+hemoglobin. Normal ranges، المدى الطبيعي أو المستوى
+
+94
+00:07:41,030 --> 00:07:46,290
+الطبيعي ل ال hemoglobin. طبعا احنا بنقيس ال
+
+95
+00:07:46,290 --> 00:07:50,250
+hemoglobin في جسمنا سواء كان أنا بحكيلك فحص
+
+96
+00:07:50,250 --> 00:07:55,350
+hemoglobin أو فحص total hemoglobin، ف it's the same،
+
+97
+00:07:55,350 --> 00:07:58,850
+نفس المعنى. طب إيش يعني total hemoglobin؟ طب ليش
+
+98
+00:07:58,850 --> 00:08:04,520
+إحنا أضفنا كلمة total قبل hemoglobin؟ لأن يا بنات
+
+99
+00:08:04,520 --> 00:08:10,360
+أنواع ال hemoglobin في خلايا ال RBCs مش واحدة،
+
+100
+00:08:10,360 --> 00:08:15,480
+تمام؟ شوفوا معايا، تتذكروا إحنا ذكرنا معلومة سابقا،
+
+101
+00:08:15,480 --> 00:08:21,730
+حكينا إنه الأطفال حديثي الولادة بيكون عندهم نوع
+
+102
+00:08:21,730 --> 00:08:25,410
+من hemoglobin اللي له high affinity مع ال oxygen،
+
+103
+00:08:25,410 --> 00:08:29,270
+عشان هيك مش بسهولة بيترك ال oxygen عند ال tissue،
+
+104
+00:08:29,270 --> 00:08:32,890
+عشان هيك ربنا عوضهم بنسبة RBCs ونسبة hemoglobin
+
+105
+00:08:32,890 --> 00:08:37,390
+عالية، بالإضافة لإنه أيضا عندهم growth spurts. طيب
+
+106
+00:08:37,390 --> 00:08:41,090
+هدول حكيت لكم عندهم نوع ال hemoglobin F،
+
+107
+00:08:41,090 --> 00:08:46,850
+hemoglobin F اللي هو ال fetal hemoglobin. أطفال حديثي
+
+108
+00:08:46,850 --> 00:08:49,830
+الولادة عندهم fetal hemoglobin هو الشائع في ال
+
+109
+00:08:49,830 --> 00:08:56,790
+RBCs. طيب بيبدأ.. بيبدأ يكبروا هؤلاء الأطفال
+
+110
+00:08:56,790 --> 00:09:01,950
+حديثي الولادة، وبيبدأ مستوى ال hemoglobin يقل عندهم،
+
+111
+00:09:02,210 --> 00:09:06,370
+ليش؟ لإنه بيصير فيه تغيير في.. في تركيب ال fetal
+
+112
+00:09:06,370 --> 00:09:10,830
+hemoglobin هذا، وبيبدأ يصير يتغير التركيب الجديد
+
+113
+00:09:10,830 --> 00:09:15,890
+اللي بينتج أو التغيير على تركيبه هذا بينتج منه
+
+114
+00:09:15,890 --> 00:09:21,140
+hemoglobin، ال affinity لل oxygen تبعته معتدلة، يعني
+
+115
+00:09:21,140 --> 00:09:25,920
+بيصير عادي، بسهولة يترك ال oxygen عند ال tissue،
+
+116
+00:09:25,920 --> 00:09:29,960
+طبعا هذا ال hemoglobin بنسميه ال adult hemoglobin،
+
+117
+00:09:29,960 --> 00:09:34,780
+اللي هو، و نسبته تقريبا 95% من خلايانا إحنا ك adults،
+
+118
+00:09:34,780 --> 00:09:38,880
+كناس.. كناس مش حديثي الولادة، يعني الأطفال، و ال
+
+119
+00:09:38,880 --> 00:09:44,420
+adult، و.. يعني اللي أكبر من شهر تقريبا أو أكبر من
+
+120
+00:09:44,420 --> 00:09:50,640
+شهرين، فهدول بيكون عندهم نسبة ال hemoglobin ال.. ال
+
+121
+00:09:50,640 --> 00:09:53,300
+.. المعتدلة، affinity تبعته، ال affinity تبعته
+
+122
+00:09:53,300 --> 00:09:57,420
+معتدلة، بتكون عالية، و هذا ال hemoglobin بسميه
+
+123
+00:09:57,420 --> 00:10:04,700
+hemoglobin A، و نسبته 95% عندنا كناس adults، تمام؟
+
+124
+00:10:04,700 --> 00:10:07,810
+طيب، في عندي أنواع أخرى طبعا، يعني أنا لما بحكي إنه
+
+125
+00:10:07,810 --> 00:10:12,990
+أنا في خلايانا في 95% أو أكثر من ال hemoglobin A،
+
+126
+00:10:12,990 --> 00:10:16,350
+فبيكون في عندي نسب ضئيلة، لاتكاد تذكر، من ال
+
+127
+00:10:16,350 --> 00:10:20,830
+hemoglobin F و ال hemoglobin A2، تمام؟ نسب ضئيلة من
+
+128
+00:10:20,830 --> 00:10:24,890
+hemoglobin F and hemoglobin A2. عشان هيك لما أجي
+
+129
+00:10:24,890 --> 00:10:29,950
+أحكي أنا بعمل فحص ل ال total hemoglobin، أنا بكون
+
+130
+00:10:29,950 --> 00:10:34,280
+عم بقياس ال hemoglobin A اللي هو ال adult hemoglobin،
+
+131
+00:10:34,280 --> 00:10:37,980
+ال hemoglobin A2 و ال hemoglobin F. طب بيختلفوا
+
+132
+00:10:37,980 --> 00:10:41,240
+بيختلفوا هذه الأنواع، و ليش إحنا هيك بنسميهم مش
+
+133
+00:10:41,240 --> 00:10:45,400
+كلهم hemoglobin و السلام؟ لأنهم بيختلفوا بتركيبهم،
+
+134
+00:10:45,400 --> 00:10:50,280
+بيختلفوا بال globin part تبعهم، مش طبعا ال alpha
+
+135
+00:10:50,280 --> 00:10:54,160
+hemoglobin أو sorry، ال hemoglobin A اللي هو ال
+
+136
+00:10:54,160 --> 00:10:57,760
+adult hemoglobin، هذا هو اللي بيتكون من اثنتين alpha
+
+137
+00:10:57,760 --> 00:11:01,650
+اثنتين beta chains. لكن ال hemoglobin A2 و ال
+
+138
+00:11:01,650 --> 00:11:06,330
+hemoglobin F تركيبهم مختلف شوية، عشان هيك احنا..
+
+139
+00:11:06,980 --> 00:11:12,440
+بنصنفهم، يعني هذا A، هذا A2، هذا F، حسب تركيب
+
+140
+00:11:12,440 --> 00:11:18,020
+ال globin part تبعهم، تمام؟ اللي هم ال globin A مكون من
+
+141
+00:11:18,020 --> 00:11:23,260
+اثنتين Alpha واثنتين Beta، لكن هم ال globin A2 مكون من
+
+142
+00:11:23,260 --> 00:11:27,820
+سلاسل أخرى، راح تتعرفوا عليها بمقررات hematology لما
+
+143
+00:11:27,820 --> 00:11:32,030
+تصيروا سنة ثالثة إن شاء الله. طبعا فإذا هذا يعني
+
+144
+00:11:32,030 --> 00:11:35,550
+إحنا ليش ذكرنا هذه المعلومات؟ علشان بس نحكي إنه لما
+
+145
+00:11:35,550 --> 00:11:39,430
+أنا بقياس ال hemoglobin، بقياس total hemoglobin، إيش
+
+146
+00:11:39,430 --> 00:11:42,650
+يعني total hemoglobin؟ يعني كل أنواع ال hemoglobin
+
+147
+00:11:42,650 --> 00:11:46,090
+اللي موجودة في ال RBCs. ليش؟ هو في أنواع؟ آه، في
+
+148
+00:11:46,090 --> 00:11:50,570
+أنواع. 95% منهم ال adult، صح؟ هو الأكثر شيوعا، هو
+
+149
+00:11:50,570 --> 00:11:53,450
+المكون من اثنتين Alpha، اثنتين Beta عندنا ك adults،
+
+150
+00:11:53,450 --> 00:11:58,770
+تمام؟ ولكن في عندنا لسه جزء ضئيل من اللي هو ال
+
+151
+00:11:58,770 --> 00:12:01,510
+hemoglobin اللي كان عندنا في ولادنا، hemoglobin F،
+
+152
+00:12:01,510 --> 00:12:05,270
+وعندنا جزء ضئيل أيضا من نوع آخر اسمه hemoglobin A2،
+
+153
+00:12:05,270 --> 00:12:09,790
+تمام؟ عشان هيك لما أحكيلك نقيس ال hemoglobin أو ال
+
+154
+00:12:09,790 --> 00:12:14,000
+total hemoglobin، it's the same، نفس المعنى. طيب،
+
+155
+00:12:14,000 --> 00:12:18,160
+الوحدة اللي أنا بعبر عنها لقياس ال hemoglobin هي
+
+156
+00:12:18,160 --> 00:12:22,780
+gram per deciliter، تمام؟ gram per deciliter.
+
+157
+00:12:22,780 --> 00:12:27,740
+تتذكروا لما شرحنا فحص السكر، حكينا إنه أنا بقياسه
+
+158
+00:12:27,740 --> 00:12:31,160
+بال milligram per deciliter. طيب ليش بقياسه بال
+
+159
+00:12:31,160 --> 00:12:34,120
+milligram per deciliter، و البروتين بال gram per
+
+160
+00:12:34,120 --> 00:12:39,540
+deciliter؟ لإنه إحنا كطبيعة عندنا نسبة البروتين
+
+161
+00:12:39,540 --> 00:12:42,680
+أعلى من النسب الأخرى من السكر والأشياء الثانية،
+
+162
+00:12:43,120 --> 00:12:48,180
+فبالتالي البروتين، نظرا لوجوده بتركيز أعلى، فإذا
+
+163
+00:12:48,180 --> 00:12:52,860
+وحدة قياسه جرام per deciliter وليس مليجرام per
+
+164
+00:12:52,860 --> 00:12:57,500
+deciliter. The normal ranges of hemoglobin depend
+
+165
+00:12:57,500 --> 00:13:01,440
+on. طبعا المدى الطبيعي للهيموجلوبين بيعتمد على عدة
+
+166
+00:13:01,440 --> 00:13:06,120
+أسباب أو عدة عوامل. إيش هي هذه العوامل؟ لسه
+
+167
+00:13:06,120 --> 00:13:09,560
+.. لسه من شوية ذكرنا إنه الأطفال حديثي الولادة
+
+168
+00:13:09,560 --> 00:13:12,700
+بيكون عندهم نسبة hemoglobin أعلى، لما بيبدأ يكبر
+
+169
+00:13:12,700 --> 00:13:15,980
+شويه، بتبدأ تقل نسبة ال hemoglobin، لإنه اتغير
+
+170
+00:13:15,980 --> 00:13:20,420
+تركيبه، فالتركيب الجديد اللي صدر صار عنده أقل
+
+171
+00:13:20,420 --> 00:13:24,140
+affinity ل ال oxygen، فصار ما فيه مش في حاجة لإنه
+
+172
+00:13:24,140 --> 00:13:32,040
+يتصنع لهم hemoglobin أكثر، تمام؟ و ال altitude.. ال
+
+173
+00:13:32,040 --> 00:13:37,850
+altitude، الارتفاع، ناس عايشة على مرتفعات عندها نقص
+
+174
+00:13:37,850 --> 00:13:41,930
+في الأكسجين، إذا جسمها هيعود بإنتاج المزيد من ال
+
+175
+00:13:41,930 --> 00:13:45,930
+RBCs، فبالتالي نسبة ال hemoglobin هتكون عالية أيضا،
+
+176
+00:13:45,930 --> 00:13:50,410
+ال gender أو ال sex of person، نوع الشخص، male أو
+
+177
+00:
+
+223
+00:17:17,340 --> 00:17:21,700
+اللي بيشتغل وهو مغمض عينيه تمام، بيشتغل على عميان
+
+224
+00:17:22,110 --> 00:17:25,530
+بدون ما يعرفوا المشكلة بس بيحسوا أن نسبة الأكسجين
+
+225
+00:17:25,530 --> 00:17:29,610
+قلت بيبدأ يصنع، إذا هذه المشكلة هتخلي الـ bone
+
+226
+00:17:29,610 --> 00:17:35,570
+marrow يصنع لي RBCs بزيادة نتيجة نقص الأكسجين، نتيجة
+
+227
+00:17:35,570 --> 00:17:38,710
+نقص الأكسجين اللي حاصل في الـ tissue فبالتالي
+
+228
+00:17:38,710 --> 00:17:42,790
+زيادة الـ RBCs هذه اللي نتجت راح ينتج عنها زيادة
+
+229
+00:17:42,790 --> 00:17:47,120
+في الـ hemoglobin level  أيضا الجفاف،
+
+230
+00:17:47,120 --> 00:17:52,720
+dehydration يعني جفاف، فقدان للماء أو لسوائل الجسم
+
+231
+00:17:52,720 --> 00:17:59,280
+نتيجة حروق أو إسهال أو vomiting، تمام؟ أي سبب
+
+232
+00:17:59,280 --> 00:18:05,740
+بيفقد.. بيفقد الجسم سوائل، إذا هذا رح يخلّي الجسم
+
+233
+00:18:05,740 --> 00:18:11,740
+.. محتويات الدم تتركز، إذا زاد تركيز الـ RBCs نتيجة
+
+234
+00:18:11,740 --> 00:18:16,200
+فقدان السوائل هذه، فقدان الـ plasma اللي موجودة في
+
+235
+00:18:16,200 --> 00:18:20,660
+الـ blood، إذا نسبة الـ RBCs هتكون أعلى، إذا نسبة الـ
+
+236
+00:18:20,660 --> 00:18:24,280
+hemoglobin هتكون عالية. living at high altitude
+
+237
+00:18:24,280 --> 00:18:29,460
+حكينا الناس اللي عايشة على مرتفعات عالية عندهم high
+
+238
+00:18:29,460 --> 00:18:35,620
+hypoxia تمام، وبالتالي هيتم تصنيع RBCs زيادة عندهم
+
+239
+00:18:35,620 --> 00:18:41,060
+و.. أيضا هيكون فيه عندهم نسبة الـ hemoglobin عالية
+
+240
+00:18:41,060 --> 00:18:46,940
+"Heavy smoking"، الناس اللي عندهم يعني بدخن كثير
+
+241
+00:18:46,940 --> 00:18:49,940
+بدخن يعني هو بيدخل.. بي.. بي.. فيه عنده نقص في
+
+242
+00:18:49,940 --> 00:18:52,980
+الأكسجين، يدخل قطران وثاني أكسيد الكربون وأول
+
+243
+00:18:52,980 --> 00:18:56,600
+أكسيد الكربون إلى جسمه بدلا من الأكسجين، هذا
+
+244
+00:18:56,600 --> 00:18:59,520
+أيضا برضه كمان الـ bone marrow راح يبدأ يصنع لي
+
+245
+00:18:59,520 --> 00:19:02,980
+RBCs زيادة عشان يعوض نقص الأكسجين، فبالتالي بيزيد
+
+246
+00:19:02,980 --> 00:19:07,280
+الـ hemoglobin تمام؟ بالإضافة إلى أنه عندهم في
+
+247
+00:19:07,280 --> 00:19:10,900
+الناس هدول عندهم نسبة hemoglobin مؤكسدة، وإحنا لما
+
+248
+00:19:10,900 --> 00:19:16,680
+بنقيس نسبة الـ hemoglobin في الفحص لازم نؤكسده يعني
+
+249
+00:19:16,680 --> 00:19:21,080
+وبعدين نقيسه، فإذا هو عندهم already مؤكسد وجاهز
+
+250
+00:19:21,080 --> 00:19:23,960
+فهذا برضه كمان هيرفع لي نسبة الـ hemoglobin عندهم في
+
+251
+00:19:23,960 --> 00:19:31,760
+الفحص. بس بدي أكتب لكم كلمة hypoxia: hypo يعني قليل
+
+252
+00:19:31,760 --> 00:19:43,870
+oxia يعني نقص أكسجين في الجسم تمام؟ low oxygen
+
+253
+00:19:43,870 --> 00:19:50,750
+level, low oxygen level تمام
+
+254
+00:19:59,680 --> 00:20:04,500
+طيب، below normal hemoglobin level أقل من الطبيعي
+
+255
+00:20:04,500 --> 00:20:09,560
+مستوى الـ hemoglobin، هنسمي هذه الحالة anemia، وهذه
+
+256
+00:20:09,560 --> 00:20:14,040
+الحالة تظهر في iron deficiency anemia  بالـ
+
+257
+00:20:14,040 --> 00:20:18,560
+deficiencies في الـ essential vitamins أو elements
+
+258
+00:20:18,560 --> 00:20:25,660
+زي يكون في عندي deficiency في vitamin B12 في
+
+259
+00:20:25,660 --> 00:20:31,310
+الحديد وفي الـ folate. طيب، الحديد و B12 و folate
+
+260
+00:20:31,310 --> 00:20:35,890
+و B6 هذه عبارة عن molecules مهمة جدا لتصنيع الـ
+
+261
+00:20:35,890 --> 00:20:42,710
+hemoglobin. عندي نقص في الـ hemoglobin بسبب أنه صار
+
+262
+00:20:42,710 --> 00:20:45,950
+في عندي نقص في الحديد، طبعا صار عندي نقص حديد أو
+
+263
+00:20:45,950 --> 00:20:50,770
+نقص في فيتامين B12، في الـ folate، في الـ B6، هذه
+
+264
+00:20:50,770 --> 00:20:56,610
+عبارة عن عناصر مهمة جدا لتصنيع الـ hemoglobin و
+
+265
+00:20:56,610 --> 00:21:02,030
+لنضوج الـ RBCs في الـ bone marrow. خلل فيهم أو نقصان
+
+266
+00:21:02,030 --> 00:21:07,790
+فيهم، مش هيتصنع hemoglobin كافي أو مش هتنضج الـ RBCs
+
+267
+00:21:07,790 --> 00:21:13,010
+بالوقت الكافي. لإنه حاصل نقص في الـ RBCs، والـ
+
+268
+00:21:13,010 --> 00:21:15,950
+RBCs أيضا هيكون محتواها فارغ من الـ hemoglobin
+
+269
+00:21:16,660 --> 00:21:21,140
+inherited hemoglobin defect، لما يكون المشكلة عندي
+
+270
+00:21:21,140 --> 00:21:25,960
+مشكلة mutation حدثت في الجين المسؤول عن تصنيع الـ
+
+271
+00:21:25,960 --> 00:21:30,840
+globin chain، الـ alpha والـ beta chain، معناها صار
+
+272
+00:21:30,840 --> 00:21:35,740
+في عندي مشكلة في تركيب الـ hemoglobin. هيأثر أيضا
+
+273
+00:21:35,740 --> 00:21:40,760
+على وظيفة هذا الـ hemoglobin وعلى وجوده، طبعا خلل
+
+274
+00:21:40,760 --> 00:21:44,500
+في الـ hemoglobin، إذا خلل في وظيفة الـ RBCs، إذا الـ
+
+275
+00:21:44,500 --> 00:21:49,860
+RBCs بتروح "trash"، الجسم بيبدأ يتخلص منها، يكسرها، إذا
+
+276
+00:21:49,860 --> 00:21:56,000
+المحصلة فقدان RBCs أو تكسير RBCs، يعني RBCs قليلة
+
+277
+00:21:56,000 --> 00:22:00,320
+يعني hemoglobin level قليل. هذه بنشوفها في حالات
+
+278
+00:22:00,320 --> 00:22:03,820
+الـ sickle cell anemia، أنيميا الخلايا المنجلية
+
+279
+00:22:03,820 --> 00:22:09,040
+والثلاسيميا، أنيميا البحر الأبيض المتوسط أو حوض البحر
+
+280
+00:22:09,040 --> 00:22:13,830
+الأبيض المتوسط. other inherited defect affecting
+
+281
+00:22:13,830 --> 00:22:18,210
+their blood cells، أي مشكلة وراثية تؤثر على الـ
+
+282
+00:22:18,210 --> 00:22:21,530
+RBCs، على شكلها، على تركيبها، على وظيفتها، على الـ
+
+283
+00:22:21,530 --> 00:22:26,830
+structure تبعها، وعلى عمرها، متوسط عمرها، أيضا كمان
+
+284
+00:22:26,830 --> 00:22:31,900
+راح يعطيني below normal hemoglobin level. الـ
+
+285
+00:22:31,900 --> 00:22:37,100
+excessive bleeding، نزيف حاد تمام؟ هذا يؤدي إلى فقدان
+
+286
+00:22:37,100 --> 00:22:43,660
+الـ RBCs، وأيضا بالتالي فقدان.. نقص في الـ RBCs و
+
+287
+00:22:43,660 --> 00:22:48,520
+below normal hemoglobin level. طبعا الجسم بيضل بعد الـ
+
+288
+00:22:48,520 --> 00:22:51,760
+bleeding فترة طويلة، وهي نسبة الـ hemoglobin عنده
+
+289
+00:22:51,760 --> 00:22:55,040
+قليلة، والـ hematocrit عنده قليل أيضا، اللي هو يعني
+
+290
+00:22:55,040 --> 00:22:59,980
+نسبة الـ RBCs. ليش؟ لأن الجسم بيعود السوائل، الـ plasma
+
+291
+00:23:00,610 --> 00:23:05,370
+بطريقة أسرع مما بيعود خلايا. excessive destruction
+
+292
+00:23:05,370 --> 00:23:08,390
+of red blood cells، لو أنا عندي مشكلة في تركيب
+
+293
+00:23:08,390 --> 00:23:15,470
+الـ RBCs، فالجسم بيتم تكسيرها بسهولة في الجسم
+
+294
+00:23:15,470 --> 00:23:21,400
+في الـ circulation تمام؟ أو حتى في الـ.. الـ spleen
+
+295
+00:23:21,400 --> 00:23:28,400
+في الطحال تمام؟ فهذا راح يؤدي إلى أن الـ RBCs قصر
+
+296
+00:23:28,400 --> 00:23:35,100
+عمرها، اتكسرت بسرعة، نقص في الـ RBCs، إذا نقص في الـ
+
+297
+00:23:35,100 --> 00:23:38,780
+hemoglobin. kidney disease، إيش يعني؟ إيش علاقة الـ
+
+298
+00:23:38,780 --> 00:23:42,560
+kidney disease في الدم؟ شوفوا معايا، من الـ
+
+299
+00:23:42,560 --> 00:23:46,140
+kidney بينتج هرمون اسمه erythropoietin أو
+
+300
+00:23:46,140 --> 00:23:50,800
+erythropoietin تمام؟ هذا الهرمون الـ erythropoietin
+
+301
+00:23:50,800 --> 00:23:57,220
+هو اللي بيروح على الـ bone marrow، ويكون حامل signal،
+
+302
+00:23:57,220 --> 00:24:01,020
+حامل signal، إشارة، بيقوله في الـ circulation
+
+303
+00:24:01,020 --> 00:24:06,860
+hypoxia، صنع RBCs زيادة عشان تحمل الأكسجين وتوديه
+
+304
+00:24:06,860 --> 00:24:15,720
+للخلايا تمام؟ إذا الاريثروبويتين هو الـ signal لتصنيع
+
+305
+00:24:15,720 --> 00:24:21,180
+الـ RBCs. عندي في الـ kidney خلل، إذا مش هيتصنع الـ
+
+306
+00:24:21,180 --> 00:24:24,720
+هرمون، هذا الاريثروبويتين، فبالتالي مش هتروح signal
+
+307
+00:24:24,720 --> 00:24:28,660
+للـ bone marrow تحكيله صنع RBCs كفاية، فبالتالي
+
+308
+00:24:28,660 --> 00:24:32,300
+هيكون في عندي نقص في الـ RBCs، و below normal
+
+309
+00:24:32,300 --> 00:24:35,740
+hemoglobin level، أقل من الطبيعي مستوى الـ
+
+310
+00:24:35,740 --> 00:24:39,830
+hemoglobin. bone marrow failure or aplastic anemia
+
+311
+00:24:39,830 --> 00:24:43,850
+فشل في الـ bone marrow، أو أنه لما الـ bone
+
+312
+00:24:43,850 --> 00:24:46,710
+marrow يصير فيه تليف، في حالة الـ aplastic
+
+313
+00:24:46,710 --> 00:24:52,090
+anemia، ممكن أيضا يصير عندي نقص في عدد الـ RBCs
+
+314
+00:24:52,090 --> 00:24:56,810
+فبالتالي below normal hemoglobin level. cancers that
+
+315
+00:24:56,810 --> 00:25:01,610
+affect the bone marrow، في عندي مشاكل ممكن يتأثر
+
+316
+00:25:01,610 --> 00:25:08,450
+على الـ bone marrow، برضه كمان ممكن إنها تسبب لي بـ
+
+317
+00:25:08,450 --> 00:25:11,050
+below normal hemoglobin level. كيف؟ إحنا من شوية
+
+318
+00:25:11,050 --> 00:25:14,350
+حكينا cancer بيعطيني polycythemia، فبتزيد الـ RBCs
+
+319
+00:25:14,350 --> 00:25:18,430
+فبيزيد الـ hemoglobin. لا، شوفوا معايا، إذا كان الـ
+
+320
+00:25:18,430 --> 00:25:23,130
+cancer في الخلايا الأم للـ RBCs، إذا المحصلة
+
+321
+00:25:23,130 --> 00:25:27,850
+polycythemia، زيادة RBCs، زيادة hemoglobin، primary
+
+322
+00:25:27,850 --> 00:25:33,280
+polycythemia. لكن إذا كان الـ cancer في الخلايا الأم
+
+323
+00:25:33,280 --> 00:25:38,020
+للـ white blood cells أو للبلايتليت، فهذه خلايا صارت cancer
+
+324
+00:25:38,020 --> 00:25:41,960
+وبدأت تتكاثر كثير وبدأت تكتسح مساحة كبيرة من
+
+325
+00:25:41,960 --> 00:25:45,660
+الـ bone marrow، على حساب مين؟ على حساب الخلايا الأم للـ
+
+326
+00:25:45,660 --> 00:25:49,540
+RBCs، بيبطل لها مكان، فبيصير الخلايا الأم للـ RBCs
+
+327
+00:25:49,540 --> 00:25:53,260
+عددها قليل في الـ bone marrow نتيجة وجود خلايا cancer
+
+328
+00:25:53,260 --> 00:25:59,170
+من نوع الـ white blood cells تمام؟ فبالتالي بيصير تصنيع الـ
+
+329
+00:25:59,170 --> 00:26:04,390
+RBCs قليل، والـ hemoglobin level قليل أيضا،
+
+330
+00:26:04,390 --> 00:26:07,530
+إذا لما بيكون في عندي حالات لوكيميا، اللي هو cancer
+
+331
+00:26:07,530 --> 00:26:11,370
+الـ white blood cells، بيكون في.. في المقابل أنيميا،
+
+332
+00:26:11,370 --> 00:26:16,670
+تمام؟ measurement of hemoglobin، إحنا كيف بنقيس
+
+333
+00:26:16,670 --> 00:26:21,390
+مستوى الـ hemoglobin؟ بنقيس الـ hemoglobin بـ cyanomet
+
+334
+00:26:21,390 --> 00:26:25,490
+hemoglobin method. طبعا بطريقة الـ cyanomet
+
+335
+00:26:25,490 --> 00:26:30,070
+hemoglobin method، كيف بالضبط؟ فكرتها أو مبدأ عملها
+
+336
+00:26:30,070 --> 00:26:34,650
+أنه الـ blood، الـ whole blood، طبعا أنا باخد عينة دم
+
+337
+00:26:34,650 --> 00:26:40,860
+كامل للفحص، بفصلها ليش؟ لأنه أنا بدي الـ RBCs، الـ RBCs
+
+338
+00:26:40,860 --> 00:26:47,480
+هذه هي اللي بدي أنا أخدها وأكسرها، ويتم
+
+339
+00:26:47,480 --> 00:26:51,860
+الحصول على الـ hemoglobin اللي فيها، فبالتالي يتفاعل
+
+340
+00:26:51,860 --> 00:26:54,620
+يعطيني مادة ملونة يتم قياسها على جهاز الـ
+
+341
+00:26:54,620 --> 00:26:58,600
+spectrophotometer. هذا الـ whole blood is diluted
+
+342
+00:26:58,600 --> 00:27:05,300
+بيتخفف بمحلول بنسميه Drabkin reagent. هذا
+
+343
+00:27:05,300 --> 00:27:09,740
+المحلول مكون من potassium ferricyanide و potassium
+
+344
+00:27:09,740 --> 00:27:15,160
+cyanide. طبعا اللي بيصير كالتالي: أن هذا المحلول
+
+345
+00:27:15,160 --> 00:27:18,560
+بيكسر الـ RBCs، بيطلع الـ hemoglobin منها، الـ
+
+346
+00:27:18,560 --> 00:27:24,580
+hemoglobin هذا بيتأكسد، بيصير له oxidized، بفعل مين؟
+
+347
+00:27:24,580 --> 00:27:27,340
+بفعل الشق الأول من الـ reagent، من مكونات الـ
+
+348
+00:27:27,340 --> 00:27:30,780
+reagent، اللي هو الـ potassium ferricyanide. طب لما
+
+349
+00:27:30,780 --> 00:27:35,700
+بيتأكسد هذا الـ hemoglobin إيش بيعطيني؟ بيعطيني met
+
+350
+00:27:35,700 --> 00:27:41,260
+hemoglobin، حاجة اسمها met-hemoglobin، طبعا؟ وهذا الـ
+
+351
+00:27:41,260 --> 00:27:44,420
+met-hemoglobin هيروح يتفاعل مع الشق الثاني من الـ
+
+352
+00:27:44,420 --> 00:27:48,360
+reagent اللي هو الـ potassium cyanide، ويتكوين لي
+
+353
+00:27:48,360 --> 00:27:53,060
+cyanomet-hemoglobin تمام؟ عشان هيك الطريقة اسمها
+
+354
+00:27:53,060 --> 00:27:57,820
+cyanomet-hemoglobin method. هذا المركب اللي نتج
+
+355
+00:27:57,820 --> 00:28:04,840
+لونه بني شوية، فبالتالي يتم قياسه على 540
+
+356
+00:28:04,840 --> 00:28:11,770
+nanometer تمام؟ على 540 nanometer. إذا الـ
+
+357
+00:28:11,770 --> 00:28:16,510
+hemoglobin اللي موجود في الدم بيكون فيه حديد
+
+358
+00:28:16,510 --> 00:28:20,650
+بالـ ferrous state، اللي هو إيش يعني؟ بحالة الـ ferrous
+
+359
+00:28:20,650 --> 00:28:24,530
+اللي هي +2، يتأكسد بفعل الـ potassium
+
+360
+00:28:24,530 --> 00:28:29,350
+ferricyanide إلى met-hemoglobin، وهذا الـ.. الـ
+
+361
+00:28:29,350 --> 00:28:32,390
+hemoglobin المؤكسد يختلف عن الـ hemoglobin
+
+362
+00:28:32,390 --> 00:28:38,590
+الطبيعي بأنه الحديد تحول من ferrous إلى ferric، من
+
+363
+00:28:38,590 --> 00:28:46,070
++2 إلى +3 تمام؟ هذا الـ met-hemoglobin
+
+364
+00:28:46,070 --> 00:28:50,810
+بفعل الـ potassium cyanide يتحول إلى cyanomet-
+
+365
+00:28:50,810 --> 00:28:57,150
+hemoglobin، colored substance، يتم قراءتها على 540
+
+366
+00:28:57,150 --> 00:29:01,810
+نانوميتر، وبنقيس طبعا كمية الـ cyanomet-hemoglobin
+
+367
+00:29:01,810 --> 00:29:06,250
+اللي هتعكس كمية الـ hemoglobin في العينة على طول
+
+368
+00:29:06,250 --> 00:29:09,350
+الموجة 540 نانوميتر، باستخدام جهاز الـ
+
+369
+00:29:09,350 --> 00:29:14,650
+spectrophotometer. الـ.. التركيز يتناسب تناسب طردي
+
+370
+00:29:14,650 --> 00:29:18,710
+مع الـ absorbance تمام؟ وهذا الكلام حكيناه و
+
+371
+00:29:18,710 --> 00:29:22,390
+شرحناه في محاضرة الـ spectrophotometer، حكينا أنه
+
+372
+00:29:22,390 --> 00:29:26,510
+أنا اللي بختار الطول الموجي اللي بدي إياه يفوت على
+
+373
+00:29:26,510 --> 00:29:31,230
+العينة، على أي أساس بختاره؟ بحيث أن المادة المراد
+
+374
+00:29:31,230 --> 00:29:36,730
+قياسها تعطيني أقصى كمية absorbance عند هذا الطول
+
+375
+00:29:36,730 --> 00:29:39,570
+الموجي. المادة اللي نتجت اللي اسمها
+
+376
+00:29:39,570 --> 00:29:43,490
+cyanomethemoglobin، اللي هتعكس لي كمية الـ hemoglobin
+
+377
+00:29:43,490 --> 00:29:48,430
+اللي تفاعل، طبعا لونها حكينا بني على بني
+
+378
+00:29:48,430 --> 00:29:53,330
+غامق، بني غامق لونها طبعا، فبالتالي هذه المادة
+
+379
+00:29:53,330 --> 00:29:59,770
+بتعطيني أقصى قيمة امتصاص على طول موجي 540، علشان هيك
+
+380
+00:29:59,770 --> 00:30:02,370
+أنا اخترت هذا الطول الموجي، أو الشركات المصنعة على
+
+381
+00:30:02,370 --> 00:30:05,950
+هذه الطريقة بتنصحني أني استخدم هذا
+
+382
+00:30:05,950 --> 00:30:10,800
+الطول الموجي للقياس. self-hemoglobin is not
+
+383
+00:30:10,800 --> 00:30:16,040
+converted by cyanomethemoglobin. طبعا في أحياناً
+
+384
+00:30:16,040 --> 00:30:24,520
+الناس بتاخد أدوية، في مكوناتها مجموعة السلفا
+
+385
+00:30:24,520 --> 00:30:28,060
+فبالتالي هذه السلفا بتتحد مع الهموجلوبين مكونة
+
+386
+00:30:28,060 --> 00:30:32,200
+مركب اسمه سلفا هموغلوبين. هذا السلفا هموغلوبين يعطي
+
+387
+00:30:32,200 --> 00:30:38,140
+لون يكسب الدم لون أخضر، وأيضا بيكون غير قابل
+
+388
+00:30:38,140 --> 00:30:41,920
+لقياسه بطريقة cyanomet hemoglobin، يعني صعب إحنا
+
+389
+00:30:41,920 --> 00:30:44,980
+نؤكسده وبعدين لـ met-hemoglobin ونحوله لـ cyanomet-
+
+390
+00:30:44,980 --> 00:30:48,800
+hemoglobin عشان نقيسه، إذا لازم نستخدم طريقة أخرى
+
+391
+00:30:48,800 --> 00:30:54,940
+لقياسه. طبعا الطريقة.. إحنا لو.. لو بتتذكروا في فحص
+
+392
+00:30:54,940 --> 00:31:00,540
+السكر، جبنا tube standard وضفت عليها reagent و
+
+393
+00:31:00,540 --> 00:31:05,460
+tube العينة وضفت عليها reagent، عملت لهم تفاعل مع
+
+3
+
+445
+00:34:38,020 --> 00:34:40,960
+الـ volume النهائي، والله هاي one ml، حطيت عليها
+
+446
+00:34:40,960 --> 00:34:44,280
+أربع ml من الـ drop كان صاروا خمسة، فهي إذا هنا
+
+447
+00:34:44,280 --> 00:34:50,800
+خمسة عندي، إذا من .. بهذه الطريقة بحسب الـ تركيز اللي
+
+448
+00:34:50,800 --> 00:34:55,860
+هو عبارة عن خمسة جرام per deciliter طبعًا، و بقيس الـ
+
+449
+00:34:55,860 --> 00:34:58,660
+absorbance على جهاز الـ spectrophotometer هاي هنا
+
+450
+00:34:58,660 --> 00:35:05,280
+ميعطيني قراءات افتراضية، طيب الـ tube رقم ثلاثة
+
+451
+00:35:05,280 --> 00:35:11,260
+اتنين و نص من الـ standard و اتنين و نص من الـ Drabkin
+
+452
+00:35:11,260 --> 00:35:14,240
+أصبح ممكن أنا برضه كمان أحسب لها تركيزها
+
+453
+00:35:14,240 --> 00:35:19,480
+بحيث أن الـ .. الـ .. الـ tube رقم ثلاثة، sorry الـ
+
+454
+00:35:19,480 --> 00:35:23,800
+tube رقم ثلاثة أنا جبت لها خمسة و عشرين، طبعًا أخذت
+
+455
+00:35:23,800 --> 00:35:27,400
+من الـ stock اللي هو تركيزه خمسة و عشرين، أخذت اتنين
+
+456
+00:35:27,400 --> 00:35:29,960
+و نص، تمام؟ و ..
+
+457
+00:35:34,300 --> 00:35:39,000
+كم هيطلع التركيز اللي احنا بدنا إياه في الـ final
+
+458
+00:35:39,000 --> 00:35:43,860
+volume عندي اتنين و نص من الـ standard و اتنين و نص
+
+459
+00:35:43,860 --> 00:35:50,140
+من الـ Drabkin يعني خمسة، و بجيب الـ تركيز بهذا الشكل
+
+460
+00:35:50,140 --> 00:35:55,240
+لباقي القنابيب، لنبوبة رقم أربعة بحط فيها اتنين
+
+461
+00:35:55,240 --> 00:36:00,940
+مل من الـ standard و tube رقم .. و بحط فيها ثلاثة
+
+462
+00:36:00,940 --> 00:36:07,660
+مل من الـ Drabkin، لـ tube رقم خمسة بحط فيها ثلاثة
+
+463
+00:36:07,660 --> 00:36:10,440
+مل من الـ standard و اتنين من الـ Drabkin، و
+
+464
+00:36:10,440 --> 00:36:13,540
+الـ tube الأخيرة أربعة مل من الـ standard و one
+
+465
+00:36:13,540 --> 00:36:19,360
+مل من الـ Drabkin، تمام؟ بهذا الشكل بحسب الـ تركيز
+
+466
+00:36:19,360 --> 00:36:23,080
+بقانون MV يساوي M1V1، دربوا إيديكم عليها واحفظوا
+
+467
+00:36:23,080 --> 00:36:27,560
+واحسبوا الـ تركيز لها، طبعًا كل tube يا بنات بروح إيش
+
+468
+00:36:27,560 --> 00:36:31,680
+بجيب؟ بجيب الـ absorbance لها على جهاز الـ
+
+469
+00:36:31,680 --> 00:36:36,340
+spectrophotometer، بعد ما أصفر على مين؟ أصفر على الـ
+
+470
+00:36:36,340 --> 00:36:41,010
+blank، على الـ zero، أصفر على الـ reagent، و أنا أنا أنا
+
+471
+00:36:41,010 --> 00:36:42,070
+أنا أنا أنا أنا أنا أنا أنا أنا أنا أنا أنا
+
+472
+00:36:42,070 --> 00:36:42,290
+أنا أنا أنا أنا أنا أنا أنا أنا أنا أنا أنا
+
+473
+00:36:42,290 --> 00:36:51,370
+أنا أنا أنا أنا أنا أنا أنا
+
+474
+00:36:54,700 --> 00:36:58,980
+فبحصل على عدة قراءات من الـ absorbance و عدة قراءات
+
+475
+00:36:58,980 --> 00:37:03,060
+من الـ concentration، هدول أنا بروح إيش بعمل؟ بطبق
+
+476
+00:37:03,060 --> 00:37:07,120
+فيه .. برسم curve طبعًا اللي أنا بعمله يا بنات، بعد ما
+
+477
+00:37:07,120 --> 00:37:10,180
+أنا أخلط الـ standard و أخلط الـ .. الـ .. الـ
+
+478
+00:37:10,180 --> 00:37:13,780
+Drabkin مع بعضهم البعض، أعمل mixing، لازم أستنى
+
+479
+00:37:13,780 --> 00:37:18,580
+عليهم عشر دقائق قبل لأقرأ الـ absorbance، بعدين بطلع
+
+480
+00:37:18,580 --> 00:37:22,840
+قراءة الـ absorbance و بروح بـ .. بعمل .. برسم curve
+
+481
+00:37:23,040 --> 00:37:28,560
+الـ curve هذا بحط الـ .. الـ absorbance على الـ y-
+
+482
+00:37:28,560 --> 00:37:32,440
+axis و الـ concentration على الـ x-axis، بهذا الشكل،
+
+483
+00:37:32,440 --> 00:37:36,660
+هاي الـ absorbance و هنا الـ concentration، فالمفروض
+
+484
+00:37:36,660 --> 00:37:41,540
+أنه العلاقة تطلع معي طردية، linear، ليش؟ لأنه
+
+485
+00:37:41,540 --> 00:37:43,840
+حكينا احنا في لما شرحنا محاضرة الـ
+
+486
+00:37:43,840 --> 00:37:47,600
+spectrophotometer، حكينا أنه الـ .. الـ .. الـ
+
+487
+00:37:47,600 --> 00:37:50,400
+absorbance يتناسب تناسب طردي مع الـ
+
+488
+00:37:50,400 --> 00:37:53,920
+concentration، و أنا لو جبت .. عملت plotting لـ
+
+489
+00:37:53,920 --> 00:37:56,320
+قيم الـ absorbance مع الـ
+
+490
+00:37:56,320 --> 00:38:01,440
+concentration، تظهر عندي علاقة linear، تمام، رسمت الـ
+
+491
+00:38:01,440 --> 00:38:05,240
+curve و طلع عندي علاقة linear، بدي .. شو بدي أعمل؟
+
+492
+00:38:05,240 --> 00:38:09,060
+بدي اروح أجيب الـ slope لهاي الـ ..
+
+493
+00:38:12,190 --> 00:38:16,510
+الـ slope لهذا الـ curve، الـ slope هو عبارة عن إيش يا
+
+494
+00:38:16,510 --> 00:38:23,310
+بنات؟ Delta Y على Delta X، فرق
+
+495
+00:38:23,310 --> 00:38:24,810
+الصادات على فرق السينات
+
+496
+00:38:27,840 --> 00:38:31,420
+هلأ من الـ .. من الـ .. من الـ slope هذا بدي أحسب
+
+497
+00:38:31,420 --> 00:38:35,920
+حسابات العينة، العينة تبعيتي، كيف بشتغلها؟ بجيب الـ
+
+498
+00:38:35,920 --> 00:38:40,080
+tube، بحط 5 ml من الـ Drabkin reagent و بضيف
+
+499
+00:38:40,080 --> 00:38:43,660
+عليهم 20 microliter .. microliter من الـ whole blood
+
+500
+00:38:43,660 --> 00:38:48,100
+بعملهم mixing منيح، بستنى 10 دقائق، بقرأ الـ
+
+501
+00:38:48,100 --> 00:38:53,660
+absorbance على 540 nanometer، و على .. طبعًا بكون
+
+502
+00:38:53,660 --> 00:38:57,150
+مسفرة على الـ blank اللي .. هو الـ tube رقم واحد في
+
+503
+00:38:57,150 --> 00:39:06,070
+الـ tubes الـ standard curve، طيب من الـ curve اللي
+
+504
+00:39:06,070 --> 00:39:10,930
+أنا رسمته، حكينا أنه أنا بدي أجيب slope و .. اللي
+
+505
+00:39:10,930 --> 00:39:13,830
+هو عبارة عن فرق الصادات على فرق السينات، هاي الـ slope
+
+506
+00:39:13,830 --> 00:39:17,290
+عبارة عن Delta Y على Delta X، فرق الصادات على فرق
+
+507
+00:39:17,290 --> 00:39:20,850
+السينات، طيب فرق الصادات هو عبارة عن إيش يا بنات؟ هو
+
+508
+00:39:20,850 --> 00:39:24,770
+فرق الـ .. فرق absorbance، طب absorbance لمين؟
+
+509
+00:39:26,590 --> 00:39:30,870
+absorbance لمين؟ الـ absorbance هذا للـ standard
+
+510
+00:39:30,870 --> 00:39:34,650
+لأنه أنا برسم standard curve أو برسم curve جاي من
+
+511
+00:39:34,650 --> 00:39:40,010
+أنابيب الـ standard، تمام؟ إذا هو عبارة عن فرق لـ
+
+512
+00:39:40,010 --> 00:39:45,810
+absorbance للـ standard، طيب فرق السينات عبارة عن إيش؟
+
+513
+00:39:45,810 --> 00:39:49,850
+فرق الـ concentration، طب الـ concentration هذا لمين؟
+
+514
+00:39:49,850 --> 00:39:53,890
+للـ standard، إذا برضه كمان فرق الـ concentration للـ
+
+515
+00:39:53,890 --> 00:39:57,790
+standard، إذا بإمكاني أن أقول Delta Y على Delta X و
+
+516
+00:39:57,790 --> 00:40:00,870
+أحكي أنه الـ slope هو عبارة عن الـ absorbance للـ
+
+517
+00:40:00,870 --> 00:40:03,630
+standard، هو عبارة عن قيمة absorbance أو فرق
+
+518
+00:40:03,630 --> 00:40:04,290
+absorbance
+
+519
+00:40:13,170 --> 00:40:16,950
+طيب لو جبت factor اللي هو عبارة عن مقلوب الـ slope،
+
+520
+00:40:16,950 --> 00:40:23,570
+هو عبارة عن مقلوب الـ slope، فأصبح عندي أنه هذا الـ
+
+521
+00:40:23,570 --> 00:40:28,570
+factor هو عبارة عن concentration للـ standard على
+
+522
+00:40:28,570 --> 00:40:32,670
+absorbance للـ standard، طيب لو أنا جبت هذا الـ
+
+523
+00:40:32,670 --> 00:40:37,190
+factor و ضربـ .. ضربته بـ .. الـ absorbance للعينة
+
+524
+00:40:37,190 --> 00:40:41,530
+المجهولة، فإيش صار عندي؟ تأملوا هذا القانون جيدًا،
+
+525
+00:40:41,530 --> 00:40:47,390
+مش هو Beer-Lambert Law؟ أصبح احنا يعني، صح، أنا
+
+526
+00:40:47,390 --> 00:40:51,750
+طبقت على أكثر من أنبوبة standard، ولكن في النهاية
+
+527
+00:40:51,750 --> 00:40:57,610
+أنا ما طلعت عن إطار Beer-Lambert Law، absorbance
+
+528
+00:40:57,610 --> 00:41:02,450
+للعينة على الـ absorbance للـ standard ضرب تركيز للـ
+
+529
+00:41:02,450 --> 00:41:07,910
+standard، هذا الـ factor في absorbance للعينة، يعني أصبح
+
+530
+00:41:07,910 --> 00:41:13,350
+أنا بهذه الطريقة بكون عم بطبق Beer-Lambert law، بس
+
+531
+00:41:13,350 --> 00:41:19,510
+بدلالة أكثر من tube standard و ليس tube واحد، تمام؟
+
+532
+00:41:19,510 --> 00:41:24,690
+هذه هي الفكرة، طيب
+
+533
+00:41:24,690 --> 00:41:30,870
+خلونا بس نقارن ما بين فحص السكر و فحص الـ
+
+534
+00:41:30,870 --> 00:41:35,440
+hemoglobin، فحص السكر كانت العينة plasma، لأن الـ
+
+535
+00:41:35,440 --> 00:41:39,980
+nutrients، جلوكوز و الأشياء المغذية، هاي بتكون
+
+536
+00:41:39,980 --> 00:41:45,420
+موجودة في الـ plasma أو الـ serum، فحص الـ hemoglobin
+
+537
+00:41:45,420 --> 00:41:48,400
+كانت العينة هي الـ blood، لأن أنا معنية أحصل على
+
+538
+00:41:48,400 --> 00:41:51,760
+خلايا، لأن الـ hemoglobin موجود داخل الـ red blood
+
+539
+00:41:51,760 --> 00:41:57,560
+cells، فحص السكر استخدمت standard tube واحدة، وما كان
+
+540
+00:41:57,560 --> 00:42:00,780
+في داعي أن هنا أرسم curve، وطبقت الـ Beer-Lambert
+
+541
+00:42:00,780 --> 00:42:06,560
+law مباشرة، لكن في فحص الـ hemoglobin أنا عملت لفة
+
+542
+00:42:06,560 --> 00:42:10,440
+هي، أي نعم، قادتني إلى أن أنا في النهاية بكون عم
+
+543
+00:42:10,440 --> 00:42:15,580
+بطبق نفس Beer-Lambert law، بس استخدمت أكثر من tube
+
+544
+00:42:15,580 --> 00:42:21,340
+standard، ورسمت curve، وانتجت أنه العلاقة ما بين الـ
+
+545
+00:42:21,340 --> 00:42:24,480
+absorbance و الـ concentration هي linear، تأكدت
+
+546
+00:42:24,480 --> 00:42:28,800
+بنفسي، وجبت الـ slope، فرق الصادات على فرق السينات،
+
+547
+00:42:28,800 --> 00:42:32,670
+جبت الـ factor اللي هو مقلوب للـ slope، ضربت هذا الـ
+
+548
+00:42:32,670 --> 00:42:37,170
+factor بـ الـ absorbance لـ test لعينة المريض، و
+
+549
+00:42:37,170 --> 00:42:40,870
+في النهاية تفاجأت أن أنا كنت عم بطبق قانون بير
+
+550
+00:42:40,870 --> 00:42:47,330
+لامبرت law بحذافيره، ولكن باستخدام أكثر من tube من الـ
+
+551
+00:42:47,330 --> 00:42:54,460
+standard، بدلالة أكثر من tube من الـ standard، الـ
+
+552
+00:42:54,460 --> 00:42:58,860
+يختلف الطول الموجي اللي أنا بقرأ عليه فحص السكر عن
+
+553
+00:42:58,860 --> 00:43:04,620
+فحص الـ hemoglobin، وأيضًا تختلف الوحدة، سكر وحدته
+
+554
+00:43:04,620 --> 00:43:08,740
+milligram per deciliter، و الـ hemoglobin وحدته
+
+555
+00:43:08,740 --> 00:43:13,550
+جرام per deciliter، يختلف الـ normal range أيضًا،
+
+556
+00:43:13,550 --> 00:43:20,430
+السكر له مستويات معينة من 70 لـ 110، و الـ hemoglobin
+
+557
+00:43:20,430 --> 00:43:24,830
+طبعًا حسب العمر و حسب الـ gender، يختلف الـ normal
+
+558
+00:43:24,830 --> 00:43:29,470
+range، تمام؟ مهم جدًا تربطوا الأمور هاي مع بعض لحتى
+
+559
+00:43:29,470 --> 00:43:32,770
+تكونوا فاهمين احنا إيش بنشتغل، وكيف بنفرق، وعلى أي
+
+560
+00:43:32,770 --> 00:43:35,570
+أساس بنختار نوع العينة أو بنختار طول الموجي، كيف
+
+561
+00:43:35,570 --> 00:43:38,290
+بشتغل، إيش .. إيش دور الـ standard، إيش دور الـ
+
+562
+00:43:38,290 --> 00:43:43,830
+blank في التجربة اللي أنا بشتغلها، discussion
+
+563
+00:43:45,170 --> 00:43:48,510
+mechanical sources of error، أول ما تسمعوا كلمة
+
+564
+00:43:48,510 --> 00:43:52,150
+mechanical sources of error، معناها مصادر الـ error
+
+565
+00:43:52,150 --> 00:43:56,850
+الخلل اللي بتحدث أثناء شغلك، الشغل اليدوي، الشغل
+
+566
+00:43:56,850 --> 00:44:00,210
+العملي، تمام؟ ممكن يكون في عندي pipetting error
+
+567
+00:44:01,000 --> 00:44:04,040
+طبعًا هنشتغل يا بنات، محلي الـ pipetting، أنا باخد
+
+568
+00:44:04,040 --> 00:44:07,020
+volume من الـ reagent على volume من العينة، volume
+
+569
+00:44:07,020 --> 00:44:09,940
+من الـ reagent على volume من الـ standard، فهذه كلها
+
+570
+00:44:09,940 --> 00:44:14,160
+باستخدام الـ automatic pipette، لازم أكون متمرسة أو
+
+571
+00:44:14,160 --> 00:44:18,380
+يعني مرنة إيدي منيح، متدربة على الـ pipette use، و
+
+572
+00:44:18,380 --> 00:44:23,680
+أيضًا بستخدمها بدقة و بشكل كثير منيح و صحيح، أيضًا
+
+573
+00:44:23,680 --> 00:44:28,080
+لازم أنا أكون منتبهة و حذرة باستخدام المحاليل، يعني
+
+574
+00:44:28,080 --> 00:44:30,880
+مثلًا الـ Drabkin reagent اللي هتستخدموه في فحص الـ
+
+575
+00:44:30,880 --> 00:44:34,660
+hemoglobin هو عبارة عن reagent، ده بيترسب يا
+
+576
+00:44:34,660 --> 00:44:38,080
+بنات، بما أنه بيترسب، إذا
+
+577
+00:44:40,700 --> 00:44:44,400
+هيكون foam، و الـ foam هذه ممكن تعيق عملية الـ pipetting
+
+578
+00:44:44,400 --> 00:44:48,260
+أو تحدث خطأ في الـ volume، ف أنا لازم أكون من
+
+579
+00:44:48,260 --> 00:44:51,020
+تبة و أنا بفضي في هذا المحلول، أفضي على الجدار و
+
+580
+00:44:51,020 --> 00:44:54,260
+شوي شوي، كمان أنا بستخدم خمسة مل من هذا الـ
+
+581
+00:44:54,260 --> 00:44:58,020
+reagent، كمية مش بسيطة، كمية كبيرة تُسكب على الـ tube،
+
+582
+00:44:58,020 --> 00:45:05,340
+فلازم أنا لما أفضي هذا الـ tube أكون يعني أكون
+
+583
+00:45:05,340 --> 00:45:08,960
+حاضرة أنه ما يَكبّر، ليت يوم هعلمكم طبعًا كيف أنا
+
+584
+00:45:08,960 --> 00:45:13,080
+بفضي و كيف باخد الـ volume، كيف بعمل pipetting في
+
+585
+00:45:13,080 --> 00:45:17,180
+الفحص، علشان تشتغلوا خطوة بخطوة، واحدة واحدة، و ما
+
+586
+00:45:17,180 --> 00:45:21,820
+تجلطوا، use of dirty or scratched
+
+587
+00:45:21,820 --> 00:45:22,520
+cuvettes
+
+588
+00:45:24,930 --> 00:45:28,730
+استخدام cuvette مش clean وفيها scratches، احنا
+
+589
+00:45:28,730 --> 00:45:31,650
+حكينا من شروط الـ Beer-Lambert law، أو الدقيقة و
+
+590
+00:45:31,650 --> 00:45:35,430
+الصحيحة، أنه يكون عندي الـ .. الـ .. الـ cuvettes
+
+591
+00:45:35,430 --> 00:45:39,250
+النّبيب اللي بحط فيها الـ tube، وهي عبارة عن sample
+
+592
+00:45:39,250 --> 00:45:43,530
+أو solution holder داخل الجهاز، لازم تكون
+
+593
+00:45:43,530 --> 00:45:47,750
+clean، مش فيها، خالية من الشقوق، من الكثور، الشطوب
+
+594
+00:45:47,750 --> 00:45:55,400
+وبالتالي ما تؤثر على مسار الضوء، إذا كان عندي الـ
+
+595
+00:45:55,400 --> 00:46:00,760
+cuvettes مش نظيفة، مساكتها بشكل خاطئ، صار في عليها
+
+596
+00:46:00,760 --> 00:46:06,600
+من الـ cotton fiber أو تلوثت، صار مشققة، مكسورة،
+
+597
+00:46:06,600 --> 00:46:10,360
+فيها خدش، هذا راح يأثر على القراءة، use of
+
+598
+00:46:10,360 --> 00:46:13,100
+deteriorated reagent
+
+599
+00:46:15,970 --> 00:46:21,210
+reagent تالف، تمام؟ reagent تالف، فاستخدام
+
+600
+00:46:21,210 --> 00:46:26,250
+reagent تالف بالتالي هيعطيني reaction non-proper
+
+601
+00:46:26,250 --> 00:46:29,030
+reaction أو improper reaction، مش الـ reaction اللي
+
+602
+00:46:29,030 --> 00:46:34,470
+بدي إياها، فبالتالي نتيجة مش صحيحة أو دقيقة، أيضًا كمان
+
+603
+00:46:34,470 --> 00:46:38,990
+في عندي أمور يجب مراعاتها أثناء إجراء الفحص، زي
+
+604
+00:46:38,990 --> 00:46:43,230
+مثلًا أنه المحلول اللي أنا بشتغل عليه أو اللي بدي
+
+605
+00:46:43,230 --> 00:46:47,470
+أقيسه يكون دائمًا clear، صح، هو ملون وأنا بدي أقيس
+
+606
+00:46:47,470 --> 00:46:51,630
+شدة اللون على الجهاز، بس لازم يكون ما فيش فيه turbidity،
+
+607
+00:46:51,630 --> 00:46:54,970
+ما فيش فيه precipitation، يعني لو شخص عنده white
+
+608
+00:46:54,970 --> 00:46:59,850
+pieces عالية كثير، هذه هتأدي إلى أن المحلول اللي
+
+609
+00:46:59,850 --> 00:47:02,870
+بدي أقيسه فيه نسبة turbidity، الـ white pieces
+
+610
+00:47:02,870 --> 00:47:08,760
+بتعمل turbidity زيادة، تمام، زيادة الـ white bcs
+
+611
+00:47:08,760 --> 00:47:13,180
+هتعمل turbidity، إذا هتأثر على الـ absorbance، هتأثر
+
+612
+00:47:13,180 --> 00:47:17,360
+على قراءة الجهاز، هيزيد الـ absorbance، فبالتالي يعطيني
+
+613
+00:47:17,360 --> 00:47:23,870
+نسبة hemoglobin falsely elevated، hemoglobin S و
+
+614
+00:47:23,870 --> 00:47:26,990
+hemoglobin C، هدول الأنواع من الـ hemoglobin ..
+
+615
+00:47:26,990 --> 00:47:29,910
+hemoglobin S هو الـ hemoglobin اللي موجود عند الناس
+
+616
+00:47:29,910 --> 00:47:33,630
+اللي مصابين بـ sickle cell anemia، الأنيميا .. بانيميا
+
+617
+00:47:33,630 --> 00:47:37,950
+الخلايا الم
+
+667
+00:51:19,840 --> 00:51:23,340
+لها high carboxy-hemoglobin  زي المدخنين، فهؤلاء
+
+668
+00:51:23,340 --> 00:51:27,880
+بيحتاج الـ hemoglobin هذا ساعة واحدة عشان
+
+669
+00:51:27,880 --> 00:51:32,260
+يتكون، ساعة مش عشر دقائق، الـ incubation period ساعة
+
+670
+00:51:32,260 --> 00:51:36,840
+عشان يتحول لـ cyano-hemoglobin، فهذا طبعاً لو أنا ..
+
+671
+00:51:36,840 --> 00:51:39,420
+لو ما كنتش عارفة أن الشخص هذا مدخن، يعني لأنه عنده
+
+672
+00:51:39,420 --> 00:51:42,840
+carboxy-hemoglobin،  و قرأت خلال عشر دقائق
+
+673
+00:51:45,910 --> 00:51:49,050
+يعني أنا ما استهلكتش ساعة كاملة عشان يتحول كل
+
+674
+00:51:49,050 --> 00:51:52,370
+الـ carboxy-hemoglobin لـ cyano-methemoglobin، إذا هقرأ
+
+675
+00:51:52,370 --> 00:51:56,250
+نتيجة أنّه مش كل الـ hemoglobin اتحول، فبالتالي ممكن يكون
+
+676
+00:51:56,250 --> 00:52:00,290
+في عندي error، بس بحكيلك أنّه أحياناً الـ degree of
+
+677
+00:52:00,290 --> 00:52:03,350
+error هذه، نسبة الـ error هذه بتكونش واضحة كتير،
+
+678
+00:52:03,350 --> 00:52:07,710
+ليش؟ لإنه احنا ذكرنا في بداية المحاضرة أنّه الـ
+
+679
+00:52:07,710 --> 00:52:12,490
+smokers هؤلاء عندهم نسبة methemoglobin عالية، عندهم دم مؤكسد
+
+680
+00:52:12,490 --> 00:52:17,910
+وجاهز تماماً؟ فهذا يعادل النسبة، بالعكس أحياناً
+
+681
+00:52:17,910 --> 00:52:22,030
+بتكون نسبتهم عالية، كمان؟ because the drug/kidney
+
+682
+00:52:22,030 --> 00:52:25,390
+agent contains cyanide، لإنه بيحتوي على الـ cyanide
+
+683
+00:52:25,390 --> 00:52:31,290
+المادة هذه سامة، خانقة، بتؤدي إلى توقف أو
+
+684
+00:52:31,290 --> 00:52:36,670
+inhibition لإنزيمات الـ glycolysis و الـ Krebs cycle
+
+685
+00:52:36,670 --> 00:52:41,810
+لإنزيمات التنفس الخلوي، عمليات الأكسدة والتمثيل الغذائي
+
+686
+00:52:41,810 --> 00:52:47,390
+اللي موجودة في الخلايا تماماً؟ فبالتالي سامة و قاتلة
+
+687
+00:52:47,390 --> 00:52:50,630
+و طبعاً irreversible يا بنات، بتعمل inhibition
+
+688
+00:52:50,630 --> 00:52:54,730
+irreversible، ولعلكم أخذتم الإنزيمات و الـ
+
+689
+00:52:54,730 --> 00:52:58,370
+inhibitors و النوع الـ reversible و الـ irreversible
+
+690
+00:52:58,370 --> 00:53:01,470
+تبعها، فالـ cyanide أحد الـ irreversible inhibitors
+
+691
+00:53:01,470 --> 00:53:06,750
+للـ cell respiratory enzymes تماماً؟ و إذا أدى إلى
+
+692
+00:53:06,750 --> 00:53:09,790
+اختناق فقتل، يعني أربعة liters من هذا الـ reagent
+
+693
+00:53:09,790 --> 00:53:14,750
+ممكن تكون قاتلة بتناولها، فبالتالي نأخذ حذرنا منه
+
+694
+00:53:14,750 --> 00:53:17,810
+أيضاً، أنّه ممكن أنّه يعمل irritation للـ skin
+
+695
+00:53:17,810 --> 00:53:23,010
+مش لازم يجي على الـ اليد، فلازم أنا أكون باستخدام يعني
+
+696
+00:53:23,010 --> 00:53:29,290
+لابسة gloves، لابسة الـ lab coat تبعي، يعني دائماً
+
+697
+00:53:29,290 --> 00:53:32,650
+باللي أنا عليه ملتزمة بكل إجراءات السلامة قبل البدء
+
+698
+00:53:32,650 --> 00:53:36,590
+في العمل، acid-free sinks should be used for
+
+699
+00:53:36,590 --> 00:53:44,720
+disposal of this reagent، لازم أنا أستخدم أو أتخلص
+
+700
+00:53:44,720 --> 00:53:49,780
+من هذا الـ reagent بأحواض، يعني الحوض أو الـ sink
+
+701
+00:53:49,780 --> 00:53:54,480
+.. يعني الحوض، الحنفية، أنّه يكون هذا لا .. يعني
+
+702
+00:53:54,480 --> 00:53:57,900
+أنا ما بسكب فيه acids، يعني ما بيتنضف بـ acids أو
+
+703
+00:53:57,900 --> 00:54:02,580
+ما بسكب فيه acids، ليش؟ لإنه إذا أنا كبيت المحلول
+
+704
+00:54:02,580 --> 00:54:05,280
+اللي هو فيه cyanide، وبعدين جيت روحت ضفت بـ chlorine أو
+
+705
+00:54:05,280 --> 00:54:09,720
+بـ acid أو كبيت acid، فمعناه الـ acid هيتفاعل، يروح
+
+706
+00:54:09,720 --> 00:54:12,440
+يتفاعل مع الـ cyanide، وهيكوّن لي الـ hydrogen cyanide
+
+707
+00:54:12,440 --> 00:54:21,020
+gas، اللي هو عبارة عن toxic gas، تماماً؟ غاز سام، فيفضل
+
+708
+00:54:21,020 --> 00:54:24,480
+أنّه أنا كمان بعد ما أنا أخلص مثلاً الأنابيب
+
+709
+00:54:24,480 --> 00:54:27,660
+التابعة، اشتغلنا فيها، خلصناها، ما ينفعش .. يعني أنبوب
+
+710
+00:54:27,660 --> 00:54:30,420
+مليان فيه خمس أمثال من الـ reagent، يعني كمية اللي
+
+711
+00:54:30,420 --> 00:54:33,760
+بقيت فيه، ما ينفعش أنا أكبها بالسلة، فبالتالي بروح أنا
+
+712
+00:54:33,760 --> 00:54:37,560
+أفرغ .. أكب محتواها بالحوض، بعدين بتخلص من الأنابيب
+
+713
+00:54:37,560 --> 00:54:43,620
+فإذا .. لازم أنّه أنا أفتح الحنفية شوية، أسيب من الماء
+
+714
+00:54:43,620 --> 00:54:46,740
+الجاري بحيث أنّه يعمل لي dilution لهذا الـ reagent
+
+715
+00:54:46,740 --> 00:54:50,960
+أنّه لو فيما بعد تم إطلاق acid أو اتنظف الحوض
+
+716
+00:54:50,960 --> 00:54:56,240
+بـ chlorine أو شيء، ما يروح يتفاعل الـ cyanide مع الـ acid
+
+717
+00:54:56,240 --> 00:54:59,040
+ويكون لي hydrogen cyanide gas اللي هو toxic gas
+
+718
+00:54:59,570 --> 00:55:03,050
+تماماً؟ إذا هتلاحظوا عليّ، أنّه أول ما بدأتوا
+
+719
+00:55:03,050 --> 00:55:06,390
+تفرغوا أو أنّه تعملوا discard لمحتوى الأنابيب
+
+720
+00:55:06,390 --> 00:55:10,270
+تلاقوني فاتحة الحنفية وسيبتها مفتوحة، ما أحد يسكرها
+
+721
+00:55:10,270 --> 00:55:14,290
+لأنّها بدها تعمل dilution لهذا الـ agent بحيث
+
+722
+00:55:14,290 --> 00:55:20,770
+أنّه ما يكون عم بنشكل أو بننتج غاز سام، أنّه بعد هيك
+
+723
+00:55:20,770 --> 00:55:26,140
+أي أحد يفوت على المختبر ما يتأذى، هكذا نكون انتهينا من
+
+724
+00:55:26,140 --> 00:55:29,700
+محاضرتنا لهذا اليوم، يعطيكم ألف عافية، ونراكم في المعمل
+
+725
+00:55:29,700 --> 00:55:30,360
+للتطبيق
diff --git a/PL9fwy3NUQKwY9jl8xw-bWkVZkWcGGyspd/ZZ8KPmQPnAc_postprocess.srt b/PL9fwy3NUQKwY9jl8xw-bWkVZkWcGGyspd/ZZ8KPmQPnAc_postprocess.srt
new file mode 100644
index 0000000000000000000000000000000000000000..c2abe569f05a97a418311cb33d32ce21f82b3a72
--- /dev/null
+++ b/PL9fwy3NUQKwY9jl8xw-bWkVZkWcGGyspd/ZZ8KPmQPnAc_postprocess.srt
@@ -0,0 +1,2900 @@
+1
+00:00:05,360 --> 00:00:08,860
+السلام عليكم ورحمة الله وبركاته طالبات العزيزات
+
+2
+00:00:08,860 --> 00:00:11,340
+اليوم ان شاء الله راح نشرح homoglobin
+
+3
+00:00:11,340 --> 00:00:14,260
+concentration determination lecture راح نكون مع
+
+4
+00:00:14,260 --> 00:00:17,920
+بعض في محاضرة بعنوان homoglobin concentration
+
+5
+00:00:17,920 --> 00:00:22,940
+determination اللي هو يعني معرفة او قياس تركيز ال
+
+6
+00:00:22,940 --> 00:00:27,140
+homoglobin و راح نتعرف كيف احنا ممكن نقيس نسبة ال
+
+7
+00:00:27,140 --> 00:00:31,480
+homoglobin في الدم وبطريقة او بتطبيق ال beer
+
+8
+00:00:31,480 --> 00:00:37,970
+lambert law homoglobin HPهذا ال HP هو عبارة عن the
+
+9
+00:00:37,970 --> 00:00:43,070
+standard abbreviation for همoglobin هو الإختصار
+
+10
+00:00:43,070 --> 00:00:47,310
+اللي متعارف عليه لبروتين الهمoglobin سواء حكيتلك
+
+11
+00:00:47,310 --> 00:00:51,350
+hemoglobin أو كتبتلك HP فأنا بكون بقصد ال
+
+12
+00:00:51,350 --> 00:00:56,070
+hemoglobin البروتين ال hemoglobin هذا هو عبارة عن
+
+13
+00:00:56,070 --> 00:01:01,430
+ال oxygen carrying pigmentهو الناقل للأكسوجين،
+
+14
+00:01:01,430 --> 00:01:05,510
+تمام؟ هو اللي بيحمل الأكسوجين داخل خلايا ال RBCs و
+
+15
+00:01:05,510 --> 00:01:09,850
+هو ال pigment .. التركيبة ال .. ال homoglobin
+
+16
+00:01:09,850 --> 00:01:15,130
+تعطيه لون أحمر، إذا هو اللي يكسب خلايا الدم
+
+17
+00:01:15,130 --> 00:01:19,590
+الحمراء لونها الأحمر و من هنا إجت تسمية ال RBCs
+
+18
+00:01:19,590 --> 00:01:24,030
+بال red blood cells، تمام؟ من لونها، من لون ال
+
+19
+00:01:24,030 --> 00:01:28,690
+homoglobin فيهالأن هذا ال homoglobin هو ال breed
+
+20
+00:01:28,690 --> 00:01:32,710
+dominant protein هو ال protein الأكثر شيوعا و
+
+21
+00:01:32,710 --> 00:01:38,370
+المحتل أكبر حجم من خلايا ال RBCs أكثر من 60% من
+
+22
+00:01:38,370 --> 00:01:44,950
+حجم ال RBCs هو عبارة عن homoglobin تمام؟ هو عبارة
+
+23
+00:01:44,950 --> 00:01:48,690
+عن homoglobin homoglobin carries oxygen from
+
+24
+00:01:48,690 --> 00:01:53,580
+places of high oxygen pressure lungsto places of
+
+25
+00:01:53,580 --> 00:01:57,660
+low oxygen pressure such as tissues ال homoglobin
+
+26
+00:01:57,660 --> 00:02:03,240
+حكينا إيش وظيفته؟ هو عبارة عن ناقل لأل أكسوجين،
+
+27
+00:02:03,240 --> 00:02:08,560
+إذا هو أثناء سرايان الدم و يذهبوا لال lungs،
+
+28
+00:02:08,560 --> 00:02:12,900
+للريقتين، في الريقتين موجود ضغط عالي من الأكسوجين،
+
+29
+00:02:12,900 --> 00:02:15,920
+تمام؟ لإن الريقتين هي اللي بتستلم الأكسوجين، نعمل
+
+30
+00:02:15,920 --> 00:02:20,100
+إحنا تنفس، شاهيكالأكسوجين بيدخل إلى الرئتين، اذاً
+
+31
+00:02:20,100 --> 00:02:23,440
+الرئتين فيها high oxygen pressure ساعتها
+
+32
+00:02:23,440 --> 00:02:26,120
+الهمoglobin لما يكون عند الرئتين أو يكون في
+
+33
+00:02:26,120 --> 00:02:31,720
+الرئتين إيش راح يعمل؟ راح يبدأ يحمل أكسوجين، تمام؟
+
+34
+00:02:31,720 --> 00:02:36,560
+الهمoglobin راح يحمل أكسوجين في الرئتين ولمّا يعمل
+
+35
+00:02:36,560 --> 00:02:41,180
+circulation هذا الهمoglobin المحمل بالأكسوجينو يصل
+
+36
+00:02:41,180 --> 00:02:46,100
+ال tissue أو ال organs أعضاء الجسم أو أنسجة الجسم
+
+37
+00:02:46,100 --> 00:02:50,440
+اللى عندها low oxygen pressure مافيش عندها oxygen
+
+38
+00:02:50,440 --> 00:02:54,780
+كفاية اش بيروح بيعمل بيبدأ يترك هذا ال oxygen اللى
+
+39
+00:02:54,780 --> 00:02:59,720
+حمله إذا ال homoglobin هي هيك خواص عمله في ال high
+
+40
+00:02:59,720 --> 00:03:06,710
+oxygen pressure بيبدأ يستلم يحمل oxygen تمام؟و في
+
+41
+00:03:06,710 --> 00:03:10,210
+ال low oxygen pressure عند ال tissue بيبدأ يترك
+
+42
+00:03:10,210 --> 00:03:14,530
+oxygen عشان هيك بنحكي عنه ناقل career، delivery
+
+43
+00:03:14,530 --> 00:03:21,250
+لال oxygenHomoglobin also returns CO2 from the
+
+44
+00:03:21,250 --> 00:03:25,890
+tissue to the lung فالمقابل هذا ال homoglobin بي
+
+45
+00:03:25,890 --> 00:03:31,290
+.. مقابل انه بيعطي ال tissue اكسوجين هو يحمل ال
+
+46
+00:03:31,290 --> 00:03:36,330
+CO2 من ال tissue و بيرجعها لل lung علشان تخرج في
+
+47
+00:03:36,330 --> 00:03:41,630
+عملية الزفير هذا ال homoglobin molecule يتكون من
+
+48
+00:03:41,630 --> 00:03:49,150
+جزئين شق بروتيني بنسميه globinهو شق نن بروتين
+
+49
+00:03:49,150 --> 00:03:54,270
+بنسمي الهيم تمام؟ عشان هيك هو اسمه hemoglobin
+
+50
+00:03:54,270 --> 00:03:59,970
+تمام؟ شق بروتيني الoglobin وشق نن بروتين اللي هو
+
+51
+00:03:59,970 --> 00:04:05,550
+الهيم هذا الهيم عبارة عن أربع حلقات من الهيم أربع
+
+52
+00:04:05,550 --> 00:04:11,370
+حلقات هيم كل واحدة منهم مرتبطة مع جزاء iron أو ذرة
+
+53
+00:04:11,370 --> 00:04:16,600
+iron iron تمام؟في الferrocystate إيش يعني في
+
+54
+00:04:16,600 --> 00:04:23,160
+الferrocystate؟ يعني iron Fe plus 2 شحنتهاو هنا
+
+55
+00:04:23,160 --> 00:04:26,840
+حاكي .. حاكيلي one molecule of globin جزء globin
+
+56
+00:04:26,840 --> 00:04:31,160
+معبر عن كل بروتين يعني على كل الشكل البروتيني عبّر
+
+57
+00:04:31,160 --> 00:04:36,220
+عنه بإن هو ال globin part تمام؟ ولكن هذا ال globin
+
+58
+00:04:36,220 --> 00:04:42,560
+part مكوّن من أربع سلاسل ببتيدية four polypeptide
+
+59
+00:04:42,560 --> 00:04:48,380
+chain تمام؟ تنتين منهم من نوع alpha chain وتنتين
+
+60
+00:04:48,380 --> 00:04:53,600
+من نوع beta chainهذه السلاسل الـ alpha chain
+
+61
+00:04:53,600 --> 00:04:57,840
+الواحدة منهم طولها 141 amino acid و ال beta
+
+62
+00:04:57,840 --> 00:05:03,980
+الواحدة منهم 146 amino acidتمام؟ طيب خلينا بس نشوف
+
+63
+00:05:03,980 --> 00:05:07,740
+مع بعض كيف هو تركيب ال homoglobin ال homoglobin
+
+64
+00:05:07,740 --> 00:05:11,060
+مكوّن من ال globin part اللي هو ال protein part
+
+65
+00:05:11,060 --> 00:05:16,360
+الجزء ال protein منه و ال hem part الحلقات ال hem
+
+66
+00:05:16,360 --> 00:05:20,900
+الأربعة اللي هي non-protein part مش .. التركيبها
+
+67
+00:05:20,900 --> 00:05:23,460
+مش protein يعني مافيش فيها peptide bond مافيش فيها
+
+68
+00:05:23,460 --> 00:05:29,030
+amino acids يعني عشان نحكي عنها بروتينيةتمام؟ طيب
+
+69
+00:05:29,030 --> 00:05:35,090
+هذا الoglobin part مكون من 4 سلاسل تنتين alpha
+
+70
+00:05:35,090 --> 00:05:40,310
+مقابل بعضهم البعض و تنتين beta تنتين alpha و تنتين
+
+71
+00:05:40,310 --> 00:05:47,170
+beta بهذا الشكل ال alpha الواحدة منهم طولها 141
+
+72
+00:05:47,170 --> 00:05:53,910
+amino acid ال beta طولها 146 amino acidوكل سلسلة
+
+73
+00:05:53,910 --> 00:05:58,170
+منها دول بتكون الواحدة منهم مرتبطة بحلقة heme
+
+74
+00:05:58,170 --> 00:06:04,790
+تمام؟ حلقة heme حلقة ال heme هذه في مركزها موجود
+
+75
+00:06:04,790 --> 00:06:09,810
+ال iron تمام؟ بالferrous state اللي هو iron Fe
+
+76
+00:06:09,810 --> 00:06:16,570
+plus 2 بيكون طبعا هذا .. عند حلقة ال iron هاي أو
+
+77
+00:06:16,570 --> 00:06:20,800
+حلقة ال heme اللي في مركزها ironهنا يرتبط ال
+
+78
+00:06:20,800 --> 00:06:25,260
+oxygen .. هنا يحمل ال oxygen على ال homoglobin
+
+79
+00:06:25,260 --> 00:06:30,540
+تمام؟ و بالمقابل طبعا في ال lung يحمل ال oxygen و
+
+80
+00:06:30,540 --> 00:06:35,300
+يتم تركه في ال tissue و .. و يحمل بداله مين CO2
+
+81
+00:06:35,300 --> 00:06:40,420
+عشان يرجع .. يرجعه على ال lung و يتم التخلص منه في
+
+82
+00:06:40,420 --> 00:06:46,870
+عملية الزفيرةa heme group is a flat ring molecule
+
+83
+00:06:46,870 --> 00:06:52,110
+containing carbon, nitrogen and hydrogen and
+
+84
+00:06:52,110 --> 00:06:57,130
+oxygen تمام؟ ال heme هي عبارة عن حلقة مسطحة من ال
+
+85
+00:06:57,130 --> 00:07:00,410
+hydrogen و ال carbon و ال .. و ال nitrogen مرتبطة
+
+86
+00:07:00,410 --> 00:07:03,590
+مع بعضها البعض و في وجود أيضا لل oxygen و هي
+
+87
+00:07:03,590 --> 00:07:09,330
+توسطها موجود عندي Fe plus 2 في الرأس molecule وين؟
+
+88
+00:07:09,330 --> 00:07:14,190
+في ال center تمام؟ في ال centerwithout the iron
+
+89
+00:07:14,190 --> 00:07:18,070
+بدون ال iron هذا نخب ال iron شوية without the iron
+
+90
+00:07:18,070 --> 00:07:23,130
+هاي الحلقة بنسميها porphyrin ring مع ال iron بتصير
+
+91
+00:07:23,130 --> 00:07:30,750
+heme without iron porphyrin with iron heme تمام؟
+
+92
+00:07:30,750 --> 00:07:35,370
+فلازم انه نعرف هاي الأساسيات عن تركيب ال
+
+93
+00:07:35,370 --> 00:07:41,030
+homoglobinnormal ranges المدى الطبيعي أو المستوى
+
+94
+00:07:41,030 --> 00:07:46,290
+الطبيعي لأ ال homoglobin طبعا احنا بنقيس ال
+
+95
+00:07:46,290 --> 00:07:50,250
+homoglobin في جسمنا سواء كان انا بحكيلك فحص
+
+96
+00:07:50,250 --> 00:07:55,350
+homoglobin او فحص total homoglobin ف it's the same
+
+97
+00:07:55,350 --> 00:07:58,850
+نفس المعنى طب ايش يعني total homoglobin؟ طب ليش
+
+98
+00:07:58,850 --> 00:08:04,520
+احنا اضفنا كلمة total قبل homoglobin؟لأن يا بنات
+
+99
+00:08:04,520 --> 00:08:10,360
+أنواع ال homoglobin في خلايا ال RBCs مش واحدة،
+
+100
+00:08:10,360 --> 00:08:15,480
+تمام؟ شوفوا معايا، تتذكروا احنا ذكرنا معلومة سابقا
+
+101
+00:08:15,480 --> 00:08:21,730
+حكينا انهالأطفال الحديثي الولادة بيكون عندهم نوع
+
+102
+00:08:21,730 --> 00:08:25,410
+ال homoglobin اللي له high affinity مع ال oxygen
+
+103
+00:08:25,410 --> 00:08:29,270
+عشان هيك مش بسهولة بيترك ال oxygen عند ال tissue
+
+104
+00:08:29,270 --> 00:08:32,890
+عشان هيك ربنا عوضهم بنسبة RBCs ونسبة homoglobin
+
+105
+00:08:32,890 --> 00:08:37,390
+عالية بالاضافة لإنه أيضا عندهم growth pairs طيب
+
+106
+00:08:37,390 --> 00:08:41,090
+هدول حكيتلكم عندهم النوع ال homoglobin F
+
+107
+00:08:41,090 --> 00:08:46,850
+homoglobin F اللي هو ال fetal homoglobinأطفال حدثي
+
+108
+00:08:46,850 --> 00:08:49,830
+الولادة عندهم فيتا الهموجلبيين هو الشائع في ال
+
+109
+00:08:49,830 --> 00:08:56,790
+RBCs طيب بيبدأ .. بيبدوا يكبروا هدول الناس الأطفال
+
+110
+00:08:56,790 --> 00:09:01,950
+حدثي الولادة وبيبدأ مستوى الهموجلبيين يقل عندهم
+
+111
+00:09:02,210 --> 00:09:06,370
+ليش؟ لإنه بيصير فيه تغيير في .. في تركيب ال fetal
+
+112
+00:09:06,370 --> 00:09:10,830
+homoglobin هذا وبيبدأ يصير اتقل التركيب الجديد
+
+113
+00:09:10,830 --> 00:09:15,890
+اللي بينتج أو التغيير على تركيبه هذا بينتج منه
+
+114
+00:09:15,890 --> 00:09:21,140
+homoglobin ال affinity لل oxygen تبعته معتدلهيعني
+
+115
+00:09:21,140 --> 00:09:25,920
+بيصير عادي بسهولة يترك ال oxygen عند ال tissue
+
+116
+00:09:25,920 --> 00:09:29,960
+طبعا هذا ال homoglobin بنسميه ال adult homoglobin
+
+117
+00:09:29,960 --> 00:09:34,780
+اللي هو و نسبته تقريبا 95% من خلايانا احنا ك adult
+
+118
+00:09:34,780 --> 00:09:38,880
+كناس .. كناس مش حدثي الولادة يعني الأطفال و ال
+
+119
+00:09:38,880 --> 00:09:44,420
+adult و .. يعني اللي أكبر من شهر تقريبا أو أكبر من
+
+120
+00:09:44,420 --> 00:09:50,640
+شهرين فهدول بيكون عندهم نسبة ال homoglobinال .. ال
+
+121
+00:09:50,640 --> 00:09:53,300
+.. المعتدلة affinity تبعته، ال affinity تبعته
+
+122
+00:09:53,300 --> 00:09:57,420
+معتدلة بتكون عالية و هذا ال homoglobin بسميه
+
+123
+00:09:57,420 --> 00:10:04,700
+homoglobin A و نسبته 95% عندنا كناس adult، تمام؟
+
+124
+00:10:04,700 --> 00:10:07,810
+طيبفي عندي أنواع أخرى طبعا يعني أنا لما بحكي أنه
+
+125
+00:10:07,810 --> 00:10:12,990
+أنا في خلايانا في 95% أو أكثر من ال homoglobin A
+
+126
+00:10:12,990 --> 00:10:16,350
+فبيكون في عندي نسب ضئيلة لاتكاد تذكر من ال
+
+127
+00:10:16,350 --> 00:10:20,830
+homoglobin F و ال homoglobin A2 تمام؟ نسب ضئيلة من
+
+128
+00:10:20,830 --> 00:10:24,890
+homoglobin F and homoglobin A2 عشان هيك لما أجي
+
+129
+00:10:24,890 --> 00:10:29,950
+أحكي أنا بعمل فحص لأ ال total homoglobin أنا بكون
+
+130
+00:10:29,950 --> 00:10:34,280
+عم بذكر ال homoglobin Aاللي هو ال adult homoglobin
+
+131
+00:10:34,280 --> 00:10:37,980
+ال homoglobin A2 و ال homoglobin F طب بقاشى
+
+132
+00:10:37,980 --> 00:10:41,240
+بيختلفوا هدول الأنواع و ليش احنا هيك بنسميهم مش
+
+133
+00:10:41,240 --> 00:10:45,400
+كلهم homoglobin و السلام لإنهم بيختلفوا بتركيبهم
+
+134
+00:10:45,400 --> 00:10:50,280
+بيختلفوا بالoglobin part تبعهم مش طبعا ال alpha
+
+135
+00:10:50,280 --> 00:10:54,160
+homoglobin او sorry ال homoglobin A اللي هو ال
+
+136
+00:10:54,160 --> 00:10:57,760
+adult homoglobin هذا هو اللي بيتكون من تنتين alpha
+
+137
+00:10:57,760 --> 00:11:01,650
+تنتين beta chainsلكن ال homoglobin A2 و ال
+
+138
+00:11:01,650 --> 00:11:06,330
+homoglobin F تركيبهم مختلف شوية عشان هيك احنا ..
+
+139
+00:11:06,980 --> 00:11:12,440
+بنصنفهم يعني هذا لأ هذا A هذا A2 هذا F حسب تركيب
+
+140
+00:11:12,440 --> 00:11:18,020
+الجلبين part تبعهم تمام اللي هم الجلبين A مكوّن من
+
+141
+00:11:18,020 --> 00:11:23,260
+تنتين Alpha وتنتين Beta لكن هم الجلبين A2 مكوّن من
+
+142
+00:11:23,260 --> 00:11:27,820
+سلاسل أخرى راح تتعرفوا عليها بمساقل hematology لما
+
+143
+00:11:27,820 --> 00:11:32,030
+تصيروا سنة تالتة ان شاء اللهطبعا فإذا هذا يعني
+
+144
+00:11:32,030 --> 00:11:35,550
+إحنا ليش ذكرنا هذه المعلومات علشان بس نحكي أنه لما
+
+145
+00:11:35,550 --> 00:11:39,430
+أنا بقيس ال homoglobin بقيس total homoglobin إيش
+
+146
+00:11:39,430 --> 00:11:42,650
+يعني total homoglobin؟ يعني كل أنواع ال homoglobin
+
+147
+00:11:42,650 --> 00:11:46,090
+اللي موجودة في ال RBCs ليش؟ هو في أنواع؟ أه في
+
+148
+00:11:46,090 --> 00:11:50,570
+أنواع95% منهم الـ adult صح هو الأكثر شيوعا هو
+
+149
+00:11:50,570 --> 00:11:53,450
+المكون من تنتين Alpha تنتين Beta عندنا ك adult
+
+150
+00:11:53,450 --> 00:11:58,770
+تمام؟ ولكن في عندنا لسه ضايق جزء من اللي هو ال
+
+151
+00:11:58,770 --> 00:12:01,510
+homoglobin اللي كان عندنا في ولادها homoglobin F
+
+152
+00:12:01,510 --> 00:12:05,270
+وعندنا جزء ضئيل أيضا من نوع آخر اسمه homoglobin A2
+
+153
+00:12:05,270 --> 00:12:09,790
+تمام؟ عشان هيك لما أحكيلك نقيس ال homoglobin أو ال
+
+154
+00:12:09,790 --> 00:12:14,000
+total homoglobin it's the same نفس المعنىطيب
+
+155
+00:12:14,000 --> 00:12:18,160
+الوحدة اللي أنا بعبر عنها لقياس ال homoglobin هي
+
+156
+00:12:18,160 --> 00:12:22,780
+gram per deciliter تمام؟ gram per deciliter
+
+157
+00:12:22,780 --> 00:12:27,740
+تتذكروا لما شرحنا فحص السكر حكينا إنه أنا بقيسه
+
+158
+00:12:27,740 --> 00:12:31,160
+بال milligram per deciliter طيب ليش بقيسه بال
+
+159
+00:12:31,160 --> 00:12:34,120
+milligram per deciliter و البروتين بال gram per
+
+160
+00:12:34,120 --> 00:12:39,540
+deciliter؟ لإنه احنا كطبيعة عندنا نسبة البروتين
+
+161
+00:12:39,540 --> 00:12:42,680
+أعلى من النسب الأخرى من السكر و الأشياء التانية
+
+162
+00:12:43,120 --> 00:12:48,180
+فبالتالي البروتين نظرا لو وجوده بتركيز أعلى فإذا
+
+163
+00:12:48,180 --> 00:12:52,860
+وحدة قياسه اجرام per deciliter وليس ميلي جرام per
+
+164
+00:12:52,860 --> 00:12:57,500
+deciliter the normal ranges of homoglobin depend
+
+165
+00:12:57,500 --> 00:13:01,440
+on طبعا المدى الطبيعي للهومoglobin بيعتمد على عدة
+
+166
+00:13:01,440 --> 00:13:06,120
+أسباب أو عدة عوامل إيش هي هذه العوامل ال edgeلسه
+
+167
+00:13:06,120 --> 00:13:09,560
+.. لسه من شويه ذكرنا انه الأطفال حديثي الولادة
+
+168
+00:13:09,560 --> 00:13:12,700
+بيكون عندهم نسبة homoglobin أعلى لما بيبدوا يكبر
+
+169
+00:13:12,700 --> 00:13:15,980
+شويه بتبدأ يتقل نسبة ال homoglobin لإنه اتغير
+
+170
+00:13:15,980 --> 00:13:20,420
+تركيبه فالتركيب الجديد اللي صدر صار عنده أقل
+
+171
+00:13:20,420 --> 00:13:24,140
+affinity لال oxygen فصار مافيهم مش في حاجة لإنه
+
+172
+00:13:24,140 --> 00:13:32,040
+يتصنعلهم homoglobin أكتر تمام؟ و ال attitude .. ال
+
+173
+00:13:32,040 --> 00:13:37,850
+altitudeالارتفاع، ناس عايش على مرتفعات عندها نقص
+
+174
+00:13:37,850 --> 00:13:41,930
+في الأكسجين، إذا جسمها هيعود بإنتاج المزيد من ال
+
+175
+00:13:41,930 --> 00:13:45,930
+RBCs فبالتالي نسبة ال homoglobin هتكون عالية أيضا
+
+176
+00:13:45,930 --> 00:13:50,410
+ال gender أو ال sex of person، نوع الشخص male أو
+
+177
+00:13:50,410 --> 00:13:54,290
+female حكينا إن ال male عندهم نسبة RBCs ونسبة
+
+178
+00:13:54,290 --> 00:13:58,890
+homoglobin أعلى، ليش؟ لإن أول إشي عندهم كتلة عضلية
+
+179
+00:13:58,890 --> 00:14:06,400
+أكثرعندهم muscle mass أعلى تمام و أيضا انه ال
+
+180
+00:14:06,400 --> 00:14:10,480
+female عندها regular blood loss تمام regular blood
+
+181
+00:14:10,480 --> 00:14:16,160
+loss normal values in an adult بتراوح ما بين اتناش
+
+182
+00:14:16,160 --> 00:14:19,980
+لجرام و بصراحة بدقة أكتر خلينا نستعرضها لحتى نعرف
+
+183
+00:14:19,980 --> 00:14:22,660
+ال normal ranges نستعرض هذا الجدول
+
+184
+00:14:25,920 --> 00:14:30,340
+أطفال حديثي الولادة وعمر أسابيع طبعا بيكون عندهم
+
+185
+00:14:30,340 --> 00:14:34,040
+نسبة ال homoglobin عالية بيبدأ يكبر شوية، بيبدأ
+
+186
+00:14:34,040 --> 00:14:38,260
+يصير .. يصير عنده مستوى أو هو المستوى ال adult من
+
+187
+00:14:38,260 --> 00:14:41,080
+ال homoglobin أو ال adult range of homoglobin يعني
+
+188
+00:14:41,080 --> 00:14:45,680
+هو ال ..ال range المتعارف عليه انه يعني ال adult
+
+189
+00:14:45,680 --> 00:14:50,640
+men من 14 ل 18 gram per deciliter و ال woman من 12
+
+190
+00:14:50,640 --> 00:14:54,240
+ل 16 gram per deciliter عندنا ال woman after
+
+191
+00:14:54,240 --> 00:14:58,000
+middle age بعد منتصف عمرها مع ال blood loss or
+
+192
+00:14:58,000 --> 00:15:02,400
+dehumidification و الحمل و الولادة و ال
+
+193
+00:15:02,400 --> 00:15:05,840
+physiological need تبعتهافبصير عندها نسبة ال
+
+194
+00:15:05,840 --> 00:15:12,300
+homoglobin من 11.7 ل 13.8 أو من 11 ل 14 gram per
+
+195
+00:15:12,300 --> 00:15:17,840
+deciliter above normal homoglobin levels is called
+
+196
+00:15:17,840 --> 00:15:22,120
+polycythemia عندي homoglobin أعلى من الطبيعي بسمي
+
+197
+00:15:22,120 --> 00:15:26,060
+هذه الحالة polycythemia و below normal homoglobin
+
+198
+00:15:26,060 --> 00:15:30,140
+level homoglobin أقل من الطبيعي بسمي هذه الحالة
+
+199
+00:15:30,140 --> 00:15:36,460
+anemiaوهذا الاشي بيذكرنا ال hematocrite لما ذكرنا
+
+200
+00:15:36,460 --> 00:15:39,240
+ال hematocrite أو شرحنا ال hematocrite حكينا إنه
+
+201
+00:15:39,240 --> 00:15:42,600
+زيادة hematocrite above the normal أعلى من ال
+
+202
+00:15:42,600 --> 00:15:46,480
+normal بسميها polycythemia و hematocrite below
+
+203
+00:15:46,480 --> 00:15:50,670
+normal أقل من ال normal بسميه anemiaهلأ إيش يعني
+
+204
+00:15:50,670 --> 00:15:55,570
+polycythemia؟ هي حالة مرضية إما نتيجة cancer أو
+
+205
+00:15:55,570 --> 00:16:01,750
+organ disorder تمام؟ وبينتج عنها زيادة في تصنيع ال
+
+206
+00:16:01,750 --> 00:16:08,160
+RBCs يعني زيادة في ال homoglobin levelوتقسم إلى
+
+207
+00:16:08,160 --> 00:16:12,160
+نوعين primary polycythemia و secondary
+
+208
+00:16:12,160 --> 00:16:15,900
+polycythemia خلينا نبدأ في ال primary polycythemia
+
+209
+00:16:15,900 --> 00:16:20,780
+ال primary يا بنات يعني الخلل في مصنع ال herpes
+
+210
+00:16:20,780 --> 00:16:25,650
+يعني في ال bone marrowهناك خلايا أم لل RBCs صار
+
+211
+00:16:25,650 --> 00:16:29,950
+فيها طفرة اكتسبت سلوك in malignant صارت cancer
+
+212
+00:16:29,950 --> 00:16:35,050
+cell صارت تتكثر بشكل مجنون تنتجلي RBCs كتير هاي ال
+
+213
+00:16:35,050 --> 00:16:38,250
+RBCs تطلع ع ال circulation في ال circulation عندي
+
+214
+00:16:38,250 --> 00:16:42,270
+عدد ب RBCs عالي إذا نسبتهم مجلوبينة عالية بسميها
+
+215
+00:16:42,270 --> 00:16:46,230
+primary polycythemia هاي الحالة أما ال secondary
+
+216
+00:16:46,230 --> 00:16:51,140
+polycythemiaقولنا organ disorder، مشكلة في عضو
+
+217
+00:16:51,140 --> 00:16:57,440
+معين، في نسيج معين، تمام؟ أو ظروف معينة، تمام؟ طيب
+
+218
+00:16:57,440 --> 00:17:01,640
+زي مثلا حكينا احنا ذكرنا ال severe lung و ال heart
+
+219
+00:17:01,640 --> 00:17:05,520
+disease، مشكلة في ال lungفاش تنفس كفاية، فاش
+
+220
+00:17:05,520 --> 00:17:09,940
+أكسوجين كفاية بيفوت على الجسم أو مشكلة في القلب،
+
+221
+00:17:09,940 --> 00:17:13,840
+فاش ضخ كافي للدم، يعني فاش توصيل أكسوجين كافي
+
+222
+00:17:13,840 --> 00:17:17,340
+للدم، هذا إيش راح يعمل؟ راح يخلي ال bone marrow
+
+223
+00:17:17,340 --> 00:17:21,700
+اللي بيشتغل و هو مغم عينيه تمام، بيشتغل على عميان
+
+224
+00:17:22,110 --> 00:17:25,530
+بدون ما يعرفون المشكلة بس بيحس ان نسبة ال oxygen
+
+225
+00:17:25,530 --> 00:17:29,610
+قلت بيبدأ يصنع، اذا هذه المشكلة هتخلى ال bone
+
+226
+00:17:29,610 --> 00:17:35,570
+marrow يصنع لي RBCs بزيادة نتيجة الhypoxia، نتيجة
+
+227
+00:17:35,570 --> 00:17:38,710
+نقص ال oxygen اللي حاصل في ال tissue فبالتالي
+
+228
+00:17:38,710 --> 00:17:42,790
+زيادة ال RBCs هذه اللي نتجات راح ينتج عنها زيادة
+
+229
+00:17:42,790 --> 00:17:47,120
+في ال homoglobin levelأيضا ال dehydration،
+
+230
+00:17:47,120 --> 00:17:52,720
+dehydration يعني جفاف، فقدان للماء أو لسوائل الجسم
+
+231
+00:17:52,720 --> 00:17:59,280
+نتيجة حروق أو دياريا أو vomiting، تمام؟ أي سبب
+
+232
+00:17:59,280 --> 00:18:05,740
+بيفقد .. بيفقد الجسم سوائل إذاهذا رح يخلّي الجسم
+
+233
+00:18:05,740 --> 00:18:11,740
+.. محتويات الدم تتركز إذا زاد تركيز ال RBCs نتيجة
+
+234
+00:18:11,740 --> 00:18:16,200
+فقدان السوائل هذه فقدان ال plasma اللي موجودة في
+
+235
+00:18:16,200 --> 00:18:20,660
+ال blood إذا نسبة ال RBCs هتكون أعلى إذا نسبة ال
+
+236
+00:18:20,660 --> 00:18:24,280
+homoglobin هتكون عالية living at high altitude
+
+237
+00:18:24,280 --> 00:18:29,460
+حكينا الناس اللي عايشة على مرتفعاتعالية عندهم high
+
+238
+00:18:29,460 --> 00:18:35,620
+boxia تمام و بالتالي هيتم تصنيع RBC's زيادة عندهم
+
+239
+00:18:35,620 --> 00:18:41,060
+و ..أيضًا هيكون فيه عندى نسبة الـ Homoglobin عالية
+
+240
+00:18:41,060 --> 00:18:46,940
+«هيلي سموكنج» الناس اللى عندهم يعني بدخنه كتير
+
+241
+00:18:46,940 --> 00:18:49,940
+بدخن يعني هو بيدخل .. بي .. بي .. في عنده نقص في
+
+242
+00:18:49,940 --> 00:18:52,980
+الـ Oxygen بدخل قتران و ثانو أكسيد الكربون و أول
+
+243
+00:18:52,980 --> 00:18:56,600
+أكسيد الكربون إلى جسمه بدلًا من الأكسوجين هذا
+
+244
+00:18:56,600 --> 00:18:59,520
+أيضًا برضه كمان الـ bone marrow راح يبدأ يصنعلي
+
+245
+00:18:59,520 --> 00:19:02,980
+RBC's زيادة عشان يعوض الـ Hypoxia فبالتالي بيزيد
+
+246
+00:19:02,980 --> 00:19:07,280
+الـ Homoglobinتمام؟ بالإضافة إلى إنه عندهم في
+
+247
+00:19:07,280 --> 00:19:10,900
+الناس هدول عندهم نسبة homoglobin مؤكسد وإحنا لما
+
+248
+00:19:10,900 --> 00:19:16,680
+بنقيس نسبة ال homoglobin في الفحص لازم نأكسده يعني
+
+249
+00:19:16,680 --> 00:19:21,080
+و بعدين نقيسه فإذا هو عندهم already مؤكسد و جاهز
+
+250
+00:19:21,080 --> 00:19:23,960
+فهذا برضه كمان هيرفعلي نسبة ال homoglobin عندهم في
+
+251
+00:19:23,960 --> 00:19:31,760
+الفحص بس بدي أكتبلكم كلمة hypoxia hypo يعني قليل
+
+252
+00:19:31,760 --> 00:19:43,870
+oxiaيعني نقص oxygen في الجسم تمام؟ low oxygen
+
+253
+00:19:43,870 --> 00:19:50,750
+level low oxygen level تمام
+
+254
+00:19:59,680 --> 00:20:04,500
+طيب blue normal homoglobin level أقل من الطبيعي
+
+255
+00:20:04,500 --> 00:20:09,560
+مستوى ال homoglobin هنسمي هاي الحالة anemia و هاي
+
+256
+00:20:09,560 --> 00:20:14,040
+الحالة تظهر في ال iron deficiency anemia بال
+
+257
+00:20:14,040 --> 00:20:18,560
+deficiencies في ال essential vitamins أو elements
+
+258
+00:20:18,560 --> 00:20:25,660
+زي يكون في عندى deficiency في vitamin B12 في
+
+259
+00:20:25,660 --> 00:20:31,310
+الفولاد و ال bisexطيب ال iron و ال B12 و ال folate
+
+260
+00:20:31,310 --> 00:20:35,890
+و ال B6 هذي عبارة عن molecules مهمة جدا لتصنيع ال
+
+261
+00:20:35,890 --> 00:20:42,710
+homoglobin عندي نقص في ال homoglobinبسبب انه صار
+
+262
+00:20:42,710 --> 00:20:45,950
+في عندي نقص في الحديد طبعا صار عندي نقص حديد او
+
+263
+00:20:45,950 --> 00:20:50,770
+نقص في فيتامين ال B12 في الفولات في ال B6 هذه
+
+264
+00:20:50,770 --> 00:20:56,610
+عبارة عن عناصر مهمة جدا لتصنيع ال homoglobin و
+
+265
+00:20:56,610 --> 00:21:02,030
+لنضوج ال RBCs في ال bone marrowخلل فيهم أو نقصان
+
+266
+00:21:02,030 --> 00:21:07,790
+فيهم مش هيتصنع homoglobin كافي أو مش هتنضج ال RBCs
+
+267
+00:21:07,790 --> 00:21:13,010
+بالوقت الكافي لإلها الحاصل نقص في ال RBCs و ال
+
+268
+00:21:13,010 --> 00:21:15,950
+RBCs أيضا هيكون محتوها فاضي من ال homoglobin
+
+269
+00:21:16,660 --> 00:21:21,140
+inherited homoglobin defect لما يكون المشكلة عندي
+
+270
+00:21:21,140 --> 00:21:25,960
+مشكلة mutation حدثت في الجين المسؤول عن تصنيع ال
+
+271
+00:21:25,960 --> 00:21:30,840
+globin chain ال alpha و ال beta chain معناها صار
+
+272
+00:21:30,840 --> 00:21:35,740
+في عندي مشكلة في ترتيب ال homoglobinهيأثر أيضًا
+
+273
+00:21:35,740 --> 00:21:40,760
+على وظيفة هذا ال homoglobin وعلى وجوده طبعًا خلل
+
+274
+00:21:40,760 --> 00:21:44,500
+في ال homoglobin إذا خلل في وظيفة ال RBCs إذا ال
+
+275
+00:21:44,500 --> 00:21:49,860
+RBCs بتروح ash الجسم بيبدأ يتخلص منها يكسرها إذا
+
+276
+00:21:49,860 --> 00:21:56,000
+المحصلة فقدان RBCs أو تكسير RBCs يعني RBCs قليلة
+
+277
+00:21:56,000 --> 00:22:00,320
+يعني homoglobin level قليلوها دي بنشوفها في حالات
+
+278
+00:22:00,320 --> 00:22:03,820
+ال sickles الانيميا، انيميا الخلايا المنجلية
+
+279
+00:22:03,820 --> 00:22:09,040
+والثلاثيميا، انيميا البحر الأبيض أو حوض البحر
+
+280
+00:22:09,040 --> 00:22:13,830
+الأبيض المتوسطother inherited defect affecting
+
+281
+00:22:13,830 --> 00:22:18,210
+their blood cell، أي مشكلة وراثية تؤثر على ال
+
+282
+00:22:18,210 --> 00:22:21,530
+RBCs، على شكلها، على تركيبها، على وظيفتها، على ال
+
+283
+00:22:21,530 --> 00:22:26,830
+structure تبعها وعلى عمرها، متوسط عمرها، أيضا كمان
+
+284
+00:22:26,830 --> 00:22:31,900
+راح يعطيني below normal homoglobin levelالـ
+
+285
+00:22:31,900 --> 00:22:37,100
+excessive bleeding نظيف حد تمام هذا يؤدي إلى فقدان
+
+286
+00:22:37,100 --> 00:22:43,660
+لل RBCs و أيضا بالتالي فقدان .. نقص في ال RBCs و
+
+287
+00:22:43,660 --> 00:22:48,520
+بلو normal homoglobin level طبعا الجسم بيضل بعد ال
+
+288
+00:22:48,520 --> 00:22:51,760
+bleeding فترة طويلة و هي نسبة ال homoglobin عنده
+
+289
+00:22:51,760 --> 00:22:55,040
+قليلة و ال hematocrit عنده قليل أيضا اللي هو يعني
+
+290
+00:22:55,040 --> 00:22:59,980
+نسبة ال RBCs ليش؟ لإن الجسم بيعود السوائل ل plasma
+
+291
+00:23:00,610 --> 00:23:05,370
+بطريقة أسرع مما بيعود خلايا excessive destruction
+
+292
+00:23:05,370 --> 00:23:08,390
+of red blood cell لو أنا فاني عندي مشكلة في تركيب
+
+293
+00:23:08,390 --> 00:23:15,470
+ال RBCs فالجسم فبتكسر بسهولة في الجسم
+
+294
+00:23:15,470 --> 00:23:21,400
+في ال circulation تمام؟ أو حتى في ال ..الـ spleen
+
+295
+00:23:21,400 --> 00:23:28,400
+في التخال تمام؟ فهذا راح يأدي إلى أن ال RBC's قصر
+
+296
+00:23:28,400 --> 00:23:35,100
+عمرها، اتكسرت بسرعة، نقص في ال RBC's إذا نقص في ال
+
+297
+00:23:35,100 --> 00:23:38,780
+homoglobinkidney disease ايش يعني ايش علاقة ال
+
+298
+00:23:38,780 --> 00:23:42,560
+kidney disease في ال blood؟ شوفوا معايا من ال
+
+299
+00:23:42,560 --> 00:23:46,140
+kidney بينتج هرمون اسمه erythropoietin او
+
+300
+00:23:46,140 --> 00:23:50,800
+erythropoietin تمام؟ هذا الهرمون ال erythropoietin
+
+301
+00:23:50,800 --> 00:23:57,220
+هو اللي بيروح على ال bone مرةو بيكون حامل signal،
+
+302
+00:23:57,220 --> 00:24:01,020
+اشهد ال signal، إشارة، بيقوله في في ال circulation
+
+303
+00:24:01,020 --> 00:24:06,860
+hypoxia صنع RBC's زيادة عشان تحمل ال oxygen وتوديه
+
+304
+00:24:06,860 --> 00:24:15,720
+للخلاياتمام؟ إذا الاريثروبيتين هو ال signal لتصنيع
+
+305
+00:24:15,720 --> 00:24:21,180
+ال RBCs عندي في ال kidney خلل، إذا مش هيتصنع ال
+
+306
+00:24:21,180 --> 00:24:24,720
+hormone هذا الاريثروبيتين فبالتالي مش هتروح signal
+
+307
+00:24:24,720 --> 00:24:28,660
+لل bone marrow تحكيله صنع RBCs كفاية فبالتالي
+
+308
+00:24:28,660 --> 00:24:32,300
+هيصير في عندي انقص في ال RBCs و below normal
+
+309
+00:24:32,300 --> 00:24:35,740
+homoglobin level أقل من الطبيعي مستوى ال
+
+310
+00:24:35,740 --> 00:24:39,830
+homoglobinboon marrow failure or aplastic anemia
+
+311
+00:24:39,830 --> 00:24:43,850
+فالأفشل في ال boon marrow أو أنه لما ال boon
+
+312
+00:24:43,850 --> 00:24:46,710
+marrow يصير في عنده تليف في حالة ال aplastic
+
+313
+00:24:46,710 --> 00:24:52,090
+anemia ممكن أيضا يصير عندى النقص في عدد ال RBCs
+
+314
+00:24:52,090 --> 00:24:56,810
+فبالتالي blue normal homoglobin level cancers that
+
+315
+00:24:56,810 --> 00:25:01,610
+affect the boon marrow في عندى مشاكل ممكن يتأثر
+
+316
+00:25:01,610 --> 00:25:08,450
+على ال boon marrowبرضه كمان ممكن انها تسببلي بـ
+
+317
+00:25:08,450 --> 00:25:11,050
+low normal homoglobin level كيف؟ احنا من شوية
+
+318
+00:25:11,050 --> 00:25:14,350
+حكينا cancer بيعطيني polycythemia فبتزيد ال RBC's
+
+319
+00:25:14,350 --> 00:25:18,430
+فبزيد ال homoglobin لأ شوفوا معايا، اذا كان ال
+
+320
+00:25:18,430 --> 00:25:23,130
+cancer في الخلايا الأم لال RBC's، اذا المحصلة
+
+321
+00:25:23,130 --> 00:25:27,850
+polycythemia زيادة RBC's زيادة homoglobin، primary
+
+322
+00:25:27,850 --> 00:25:33,280
+polycythemiaلكن إذا كان ال cancer في الخلايا الأم
+
+323
+00:25:33,280 --> 00:25:38,020
+لل white bc أو ل البليتلت فهذه خلايا صارت cancer
+
+324
+00:25:38,020 --> 00:25:41,960
+بدا تتكاثر كتير و بدا تكتسح مساحة كتير كبيرة من
+
+325
+00:25:41,960 --> 00:25:45,660
+البون مارو على حساب مين؟ على حساب الخلايا الأم لل
+
+326
+00:25:45,660 --> 00:25:49,540
+Rbc ببطل إلها وساق فبيصير الخلايا الأم لل Rbc
+
+327
+00:25:49,540 --> 00:25:53,260
+عددها قليل في البون مارو نتيجة وجود خلايا cancer
+
+328
+00:25:53,260 --> 00:25:59,170
+من نقل لل white bcتمام؟ فبالتالي بيصير تصنيع ال
+
+329
+00:25:59,170 --> 00:26:04,390
+RBCs قليل و .. و ال homoglobin level قليل أيضا،
+
+330
+00:26:04,390 --> 00:26:07,530
+اذا لما بيكون في عندي حالات لوكيميا اللي هو cancer
+
+331
+00:26:07,530 --> 00:26:11,370
+ال white disease بيكون في .. في المقابل أنيميا،
+
+332
+00:26:11,370 --> 00:26:16,670
+تمام؟measurement of homoglobin احنا كيف بنقيس
+
+333
+00:26:16,670 --> 00:26:21,390
+مستوى ال homoglobin؟ بنقيس ال homoglobin بـ cyano
+
+334
+00:26:21,390 --> 00:26:25,490
+met homoglobin method طبعا بطريقة ال cyano met
+
+335
+00:26:25,490 --> 00:26:30,070
+homoglobin method كيف بالظبط؟ فكرتها او مبدأ عملها
+
+336
+00:26:30,070 --> 00:26:34,650
+انه ال blood ال holy blood طبعا انا باخد عين الدم
+
+337
+00:26:34,650 --> 00:26:40,860
+كامل للفحص بفصلوشليش؟ لأنه أنا بدي ال RBCs ال RBCs
+
+338
+00:26:40,860 --> 00:26:47,480
+هذه هي اللي بدي أنا أخد .. أخدها و .. أكسرها و يتم
+
+339
+00:26:47,480 --> 00:26:51,860
+حصول على ال homoglobin اللي فيها فبالتالي يتفاعل
+
+340
+00:26:51,860 --> 00:26:54,620
+يعطيلي مادة ملونة يتم قياسها على جهاز ال
+
+341
+00:26:54,620 --> 00:26:58,600
+spectrophotometerهذا ال holy blood is diluted
+
+342
+00:26:58,600 --> 00:27:05,300
+بيتخفف بقاش بمحلول بنسميه Drabkin reagent هذا
+
+343
+00:27:05,300 --> 00:27:09,740
+المحلول مكون من potassium ferricyanide و potassium
+
+344
+00:27:09,740 --> 00:27:15,160
+cyanideطبعا اللي بيصير كالتالي انه هذا المحلول
+
+345
+00:27:15,160 --> 00:27:18,560
+بيكسر ال RBC's بيطلع ال homoglobin منها ال
+
+346
+00:27:18,560 --> 00:27:24,580
+homoglobin هذا بيتأكسد بيصيرله oxidized بفعل مين؟
+
+347
+00:27:24,580 --> 00:27:27,340
+بفعل الشق الأول من ال reagent .. من مكونات ال
+
+348
+00:27:27,340 --> 00:27:30,780
+reagent اللي هو ال potassium ferricyanide طب لما
+
+349
+00:27:30,780 --> 00:27:35,700
+بيتأكسد هذا ال homoglobin اش بيعطيني؟ بيعطيني met
+
+350
+00:27:35,700 --> 00:27:41,260
+homoglobin حاجة اسمها met homoglobin طبعا؟و هذا ال
+
+351
+00:27:41,260 --> 00:27:44,420
+met-homoglobin هيروح يتفاعل مع الشق الثاني من ال
+
+352
+00:27:44,420 --> 00:27:48,360
+reagent اللي هو ال potassium cyanide و يتوين لي
+
+353
+00:27:48,360 --> 00:27:53,060
+cyanomet-homoglobin تمام؟ عشان هيك الطريقة اسمها
+
+354
+00:27:53,060 --> 00:27:57,820
+cyanomet-homoglobin method هذا المركب اللي نتج
+
+355
+00:27:57,820 --> 00:28:04,840
+لونه على brown شوية فبالتالي يتم قياسه على 540
+
+356
+00:28:04,840 --> 00:28:11,770
+nanometer تمام؟ على 540 nanometerإذا الـ
+
+357
+00:28:11,770 --> 00:28:16,510
+Homoglobin اللي موجود في ال blood بيكون في iron
+
+358
+00:28:16,510 --> 00:28:20,650
+بالferrous state اللي هو إيش يعني؟ بحالة الferrous
+
+359
+00:28:20,650 --> 00:28:24,530
+اللي هي plus 2 يتأكسد بفعل ال potassium
+
+360
+00:28:24,530 --> 00:28:29,350
+ferricyanide إلى met homoglobin وهذا ال .. ال
+
+361
+00:28:29,350 --> 00:28:32,390
+homoglobin المؤكسد بما يختلف عن ال homoglobin
+
+362
+00:28:32,390 --> 00:28:38,590
+الطبيعي بإنه ال iron تحول من ferrous إلى ferric من
+
+363
+00:28:38,590 --> 00:28:46,070
+plus 2لأ plus تلاتة تمام؟هذا الـ met-homoglobin
+
+364
+00:28:46,070 --> 00:28:50,810
+بفعل الـ potassium cyanide يتحول إلى cyanomet
+
+365
+00:28:50,810 --> 00:28:57,150
+-homoglobin colored substance يتم قراءتها على 540
+
+366
+00:28:57,150 --> 00:29:01,810
+نانومتر وبنقيص طبعاً كمية الـ cyanomet-homoglobin
+
+367
+00:29:01,810 --> 00:29:06,250
+اللي هتعكس لكمية الـ homoglobin في العينة على طول
+
+368
+00:29:06,250 --> 00:29:09,350
+الموج 540 نانومتر باستخدام جهاز الـ
+
+369
+00:29:09,350 --> 00:29:14,650
+spectrophotometerال .. التركيز يتناسب تناسب ترضي
+
+370
+00:29:14,650 --> 00:29:18,710
+مع ال absorbance تمام؟ و هذا الكلام حكيناه و
+
+371
+00:29:18,710 --> 00:29:22,390
+شرحناه في محاضرة ال spectrophotometer حكينا إنه
+
+372
+00:29:22,390 --> 00:29:26,510
+أنا اللي بختار الطول الموجي اللي بدياه يفوت على
+
+373
+00:29:26,510 --> 00:29:31,230
+العينة على أي أساس بختاره بحيث إنه المادة المراد
+
+374
+00:29:31,230 --> 00:29:36,730
+قياسها تعطيني أقصى كمية absorbance عند هذا الطول
+
+375
+00:29:36,730 --> 00:29:39,570
+الموجيالمادة اللى نتجت اللى اسمها
+
+376
+00:29:39,570 --> 00:29:43,490
+cyanomethomoglobin اللى هتعكسلى كمية ال homoglobin
+
+377
+00:29:43,490 --> 00:29:48,430
+اللى اتفاعل طبعا لونها حكينا brown على brown
+
+378
+00:29:48,430 --> 00:29:53,330
+شريدلا براون هك لونها طبعا فبالتالي هذه المادة
+
+379
+00:29:53,330 --> 00:29:59,770
+بتعطينى أقصى قيمة امتصاص على طول موجى 540 علشان هك
+
+380
+00:29:59,770 --> 00:30:02,370
+انا اخترت هذا الطول الموجى او الشركات المصنعة على
+
+381
+00:30:02,370 --> 00:30:05,950
+الفعس هذا او الطريقة هاى بتنصحنى ان انا استخدم هذا
+
+382
+00:30:05,950 --> 00:30:10,800
+الطول الموجى للقياسself-hemoglobin is not
+
+383
+00:30:10,800 --> 00:30:16,040
+converted by cyanomethamoglobin طبعا في أحيانا
+
+384
+00:30:16,040 --> 00:30:24,520
+الناس بتاخد أدوية في من مكوناتهامجموعة الصلفة
+
+385
+00:30:24,520 --> 00:30:28,060
+فبالتالي هذه الصلفة بتتاحت مع الهموجلبين مكونة
+
+386
+00:30:28,060 --> 00:30:32,200
+مركبة اسمه صلف هموجلبين هذا الصلف هموجلبين يعطي
+
+387
+00:30:32,200 --> 00:30:38,140
+لون يكسب ال blood لون أخضر وأيضا بيكون غير قابل
+
+388
+00:30:38,140 --> 00:30:41,920
+قياسه بطريقة سيانوم الهموجلبين يعني صعب احنا
+
+389
+00:30:41,920 --> 00:30:44,980
+نأكسده وبعدين ل مت هموجلبين ونحوله لسيانوم
+
+390
+00:30:44,980 --> 00:30:48,800
+الهموجلبين عشان نقياسه إذا لازم نستخدم طريقة أخرى
+
+391
+00:30:48,800 --> 00:30:54,940
+لقياسهطبعاً الطريقة احنا لو .. لو بتتذكروا بفحص
+
+392
+00:30:54,940 --> 00:31:00,540
+السكر جيبنا tube standard وضفت عليها reagent و
+
+393
+00:31:00,540 --> 00:31:05,460
+tube العينة وضفت عليها reagent اعملتلهم تفاعل مع
+
+394
+00:31:05,460 --> 00:31:10,280
+بعض incubation لفترة معينة ظهر لونسفرت على ال
+
+395
+00:31:10,280 --> 00:31:15,440
+blank اللي هو ال reagent لحاله عشان ألغي اللون
+
+396
+00:31:15,440 --> 00:31:18,320
+اللي جاي من ال reagent و بقيس ال absorbance لل
+
+397
+00:31:18,320 --> 00:31:21,780
+standard و ال absorbance لل test و بروح أطبق في
+
+398
+00:31:21,780 --> 00:31:27,260
+Beer Lambert لو هذا بالنسبة لفحص السكر لكن بالنسبة
+
+399
+00:31:27,260 --> 00:31:31,980
+لفحص ال homoglobin الأمر مختلف نوعا مابدل ما انا
+
+400
+00:31:31,980 --> 00:31:35,740
+اجيب tube واحدة standard بجيب قنابيب من ال
+
+401
+00:31:35,740 --> 00:31:39,620
+standard طبعا بعمل عشان بدي اعمل حاجة اسمها
+
+402
+00:31:39,620 --> 00:31:43,680
+standard curve preparation هلأ ال standard بيجيني
+
+403
+00:31:43,680 --> 00:31:49,080
+مركز من الشركة بتركيز 25 gram per deciliter جاهز
+
+404
+00:31:49,080 --> 00:31:52,660
+محلول homoglobin معلوم التركيز لإن أنا بفحص
+
+405
+00:31:52,660 --> 00:31:56,140
+homoglobin في العينة فلازم المحلول يكون homoglobin
+
+406
+00:31:56,340 --> 00:31:59,220
+لأن في النهاية أنا بدي أقيس اللون اللي نتج من
+
+407
+00:31:59,220 --> 00:32:04,420
+المادة cyanomet-homoglobin فلازم ال standard أيضًا
+
+408
+00:32:04,420 --> 00:32:07,640
+يكون فيه homoglobin عشان يتفاعل مع ال reagent
+
+409
+00:32:07,640 --> 00:32:10,940
+ويعطيهم ال cyanomet-homoglobin اللي أيضًا تقاسم
+
+410
+00:32:10,940 --> 00:32:17,860
+على ال 540 nanometer زيها زي العينةتمام؟ اللهم إنه
+
+411
+00:32:17,860 --> 00:32:22,440
+في اختلاف بسيط هنا إنه بدل إطباء طيوب واحدة من ال
+
+412
+00:32:22,440 --> 00:32:26,700
+standard أنا بحضر عدة قنابيب هذه القنابيب فيها
+
+413
+00:32:26,700 --> 00:32:31,960
+طبعا تركيز متدرجة من ال standard من ال homoglobin
+
+414
+00:32:31,960 --> 00:32:35,440
+طبعا هو ال standard محلول homoglobin إذا هتكون فيه
+
+415
+00:32:35,440 --> 00:32:39,180
+تركيز متدرجة من ال homoglobin في هذه التخفيفات من
+
+416
+00:32:39,180 --> 00:32:45,400
+ال standardبجيب 6 قناة بيبليك يوم الأولى هحط فيها
+
+417
+00:32:45,400 --> 00:32:49,720
+zero من ال standard و خمسة أمقال من ال Drapkin
+
+418
+00:32:49,720 --> 00:32:52,880
+reagent ليه Drapkin reagent؟ هو عبارة عن إيش؟ هو
+
+419
+00:32:52,880 --> 00:32:57,940
+عبارة عن ال potassium ferricyanide و ال potassium
+
+420
+00:32:57,940 --> 00:33:02,230
+cyanide هدول حكينا إيش اسمهم؟Drapkin reagent إذا
+
+421
+00:33:02,230 --> 00:33:05,790
+ال reagent اللي يستخدم في فحص ال homoglobin اسمه
+
+422
+00:33:05,790 --> 00:33:09,590
+Drapkin reagent ومهم جدا تحفظوها الكلمة لإنها
+
+423
+00:33:09,590 --> 00:33:12,570
+بتضالها مستمرة معاكم كل ما بدك تشتغلي homoglobin
+
+424
+00:33:12,570 --> 00:33:17,050
+أو تشتغلي CBC أو كذا لازم ينذكر ل Drapkin reagent
+
+425
+00:33:17,050 --> 00:33:23,710
+تمام؟طيب، في هذه الحالة أنا بدي أصفر عليها،
+
+426
+00:33:23,710 --> 00:33:26,410
+عالجهاز، لإنه مافيش فيها غير dropkin reagent، بدي
+
+427
+00:33:26,410 --> 00:33:29,930
+ألغي اللون اللي جاي من ال reagent هذا ال dropkin
+
+428
+00:33:29,930 --> 00:33:33,630
+reagent يا بنات لونه على أصفر، تمام؟ و هذا اللون
+
+429
+00:33:33,630 --> 00:33:36,950
+الأصفر ممكن يعطي أثر على النتائج لإن هو عبارة عن
+
+430
+00:33:36,950 --> 00:33:41,390
+محلول ملون لحاله، من دون تفاعل هو محلول ملون، فأنا
+
+431
+00:33:41,390 --> 00:33:45,360
+لازم ألغي اللون اللي جاي منه، تمام؟فعشان هيك أنا
+
+432
+00:33:45,360 --> 00:33:48,860
+بصفر عليها و لإنه أيضا تركيزي ال homoglobin فيه
+
+433
+00:33:48,860 --> 00:33:52,700
+صفر أنا ماحطيت standard فهو هذا ال zero تبعي هو ال
+
+434
+00:33:52,700 --> 00:33:55,580
+.. ال .. ال blank تبعي اللي أنا لازم أصفر عليه
+
+435
+00:33:55,580 --> 00:33:58,360
+الاشي .. ال tube الفاضية من العينة أو من المادة
+
+436
+00:33:58,360 --> 00:34:03,630
+المراد قياسها بسميها blankطيب ال tube رقم 2 إيش
+
+437
+00:34:03,630 --> 00:34:07,230
+حطيت فيها؟ حطيت فيها one Amol من ال standard و 4
+
+438
+00:34:07,230 --> 00:34:10,930
+Amol من ال Drabkin طيب خلينا نشوف طبعا من وين أنا
+
+439
+00:34:10,930 --> 00:34:15,110
+أخدت ال standard هذا؟ أخدته من ال stock اللي عندي
+
+440
+00:34:15,110 --> 00:34:18,770
+اللي تركيزه 25 gram per deciliter طيب أنا كيف ممكن
+
+441
+00:34:18,770 --> 00:34:24,330
+أحسب تركيز ال tube رقم 2؟ على طول بطبق قانون ال mv
+
+442
+00:34:24,330 --> 00:34:29,220
+يساوي mvال .. ال tube او ال .. ال stock عندي كان
+
+443
+00:34:29,220 --> 00:34:34,740
+تركيزه 25، كام أخدت منه؟ أخدت منه one ml، كام أصبح
+
+444
+00:34:34,740 --> 00:34:38,020
+التركيز؟ هذا اللي بدي أعرفه، طب التركيز .. ال ..
+
+445
+00:34:38,020 --> 00:34:40,960
+ال volume النهائي، والله هاي one ml، حطيت عليها
+
+446
+00:34:40,960 --> 00:34:44,280
+أربع ml من ال drop كان صاروا خمسة، فهي اذا هنا
+
+447
+00:34:44,280 --> 00:34:50,800
+خمسة عندي، اذا من .. بهذه الطريقة بحسبالتركيز اللي
+
+448
+00:34:50,800 --> 00:34:55,860
+هو عبارة عن خمسة جرام per deciliter طبعا و بقيس ال
+
+449
+00:34:55,860 --> 00:34:58,660
+absorbance على جهاز ال spectrophotometer هاي هانا
+
+450
+00:34:58,660 --> 00:35:05,280
+ميعطيني قراءات افتراضية طيب ال youtube رقم تلاتة
+
+451
+00:35:05,280 --> 00:35:11,260
+اتنين و نصو من ال standard و اتنين و نص من ال led
+
+452
+00:35:11,260 --> 00:35:14,240
+rapkin اصبح ممكن انا برضه كمان احسب لها تركيزها
+
+453
+00:35:14,240 --> 00:35:19,480
+بحيث انه ال .. ال .. ال tube رقم تلاتة sorry ال
+
+454
+00:35:19,480 --> 00:35:23,800
+tube رقم تلاتة انا جبتلها خمسة و عشرين طبعا اخدت
+
+455
+00:35:23,800 --> 00:35:27,400
+من ال stock اللي هو تركيزه خمسة و عشرين اخدت اتنين
+
+456
+00:35:27,400 --> 00:35:29,960
+و نص تمام؟ و ..
+
+457
+00:35:34,300 --> 00:35:39,000
+كم هيطلع التركيز هو اللي احنا بدناياه في ال final
+
+458
+00:35:39,000 --> 00:35:43,860
+volume عندى اتنين و نص من ال standard و اتنين و نص
+
+459
+00:35:43,860 --> 00:35:50,140
+من ال drafting يعني خمسة و بجيب التركيزبهذا الشكل
+
+460
+00:35:50,140 --> 00:35:55,240
+لباقي القنابيب لنبوبة رقم أربعة بحط فيها اتنين
+
+461
+00:35:55,240 --> 00:36:00,940
+أمقال من ال standard وتيوب رقم .. و بحط فيها تلاتة
+
+462
+00:36:00,940 --> 00:36:07,660
+أمقال من ال drapkin لتيوب رقم خمسة بحط فيها تلاتة
+
+463
+00:36:07,660 --> 00:36:10,440
+أمقال من ال standard و اتنين من ال drapkin و
+
+464
+00:36:10,440 --> 00:36:13,540
+الأتيوب الأخيرة أربعة أمقال من ال standard و one
+
+465
+00:36:13,540 --> 00:36:19,360
+أمقال من ال drapkinتمام؟ بهذا الشكل بحسب التركيز
+
+466
+00:36:19,360 --> 00:36:23,080
+بقانون MV يساوي MPV دربوا إيداكم عليها واحفظوا او
+
+467
+00:36:23,080 --> 00:36:27,560
+احسبوا التركيز لإلها طبعا كل تيوب يا بنات بروح إيش
+
+468
+00:36:27,560 --> 00:36:31,680
+بجيب؟ بجيب ال absorbance لإلها على جهاز ال
+
+469
+00:36:31,680 --> 00:36:36,340
+spectrophotometer بعد ما أصفر على مين؟ أصفر على ال
+
+470
+00:36:36,340 --> 00:36:41,010
+blank على ال zero أصفر على ال reagentو انا انا انا
+
+471
+00:36:41,010 --> 00:36:42,070
+انا انا انا انا انا انا انا انا انا انا انا انا
+
+472
+00:36:42,070 --> 00:36:42,290
+انا انا انا انا انا انا انا انا انا انا انا انا
+
+473
+00:36:42,290 --> 00:36:51,370
+انا انا انا انا انا انا انا
+
+474
+00:36:54,700 --> 00:36:58,980
+فبحصل على عدة قراءات من ال absorbance و عدة قراءات
+
+475
+00:36:58,980 --> 00:37:03,060
+من ال concentration هدول أنا بروح إيش بعمل؟ بطبق
+
+476
+00:37:03,060 --> 00:37:07,120
+فيه .. برسم curve طبعا اللي أنا بيبيا بنات بعد ما
+
+477
+00:37:07,120 --> 00:37:10,180
+أنا أخلط ال standard و أخلط ال .. ال .. ال
+
+478
+00:37:10,180 --> 00:37:13,780
+drafting مع بعضهم البعض أعمل mixing لازم أستنى
+
+479
+00:37:13,780 --> 00:37:18,580
+عليهم عشر دقائق قبل لأقرأ ال absorbance بعدين بطلع
+
+480
+00:37:18,580 --> 00:37:22,840
+قراءة ال absorbance و بروح ب .. بعمل .. برسم curve
+
+481
+00:37:23,040 --> 00:37:28,560
+الـ curve هذا بحط ال .. ال absorbance على ال y
+
+482
+00:37:28,560 --> 00:37:32,440
+-axis و ال concentration على ال x-axis بهذا الشكل،
+
+483
+00:37:32,440 --> 00:37:36,660
+هاي ال absorbance و هنا ال concentration فالمفروض
+
+484
+00:37:36,660 --> 00:37:41,540
+إنه العلاقة تطلع معايا طردية، linearليش؟ لأنه
+
+485
+00:37:41,540 --> 00:37:43,840
+حكينا احنا في لما شرحنا محاضرة ال
+
+486
+00:37:43,840 --> 00:37:47,600
+spectrophotometer حكينا انه ال .. ال .. ال
+
+487
+00:37:47,600 --> 00:37:50,400
+absorbance يتناسب تناسب انترضيا مع ال
+
+488
+00:37:50,400 --> 00:37:53,920
+concentration و انا لو جبت .. عملت plotting لأ
+
+489
+00:37:53,920 --> 00:37:56,320
+سالب لك جيتي اللي هو ال absorbance مع ال
+
+490
+00:37:56,320 --> 00:38:01,440
+concentration تظهر عندي علاقة linear تمام رسمت ال
+
+491
+00:38:01,440 --> 00:38:05,240
+curve و طلع عندي علاقة linear بدي .. اش بدي اعمل؟
+
+492
+00:38:05,240 --> 00:38:09,060
+بدي اروح اجيب ال slope لهاي ال ..
+
+493
+00:38:12,190 --> 00:38:16,510
+السلوب لهذا ال curve السلوب هو عبارة عن ايش يا
+
+494
+00:38:16,510 --> 00:38:23,310
+بنات؟ Delta Y على Delta X فرق
+
+495
+00:38:23,310 --> 00:38:24,810
+الصادات على فرق الصين
+
+496
+00:38:27,840 --> 00:38:31,420
+هلأ من ال .. من ال .. من ال slope هذا بدي أحسب
+
+497
+00:38:31,420 --> 00:38:35,920
+حسابات العينة العينة تبعتي كيف بشتغلها؟ بجيب ال
+
+498
+00:38:35,920 --> 00:38:40,080
+fit you بحط 5 ml من ال Drabkin reagent و بضيف
+
+499
+00:38:40,080 --> 00:38:43,660
+عليهم 20 microliter .. microliter من ال holy blood
+
+500
+00:38:43,660 --> 00:38:48,100
+بعملهم mixing منيح بستن 10 دقائق بقرأ ال
+
+501
+00:38:48,100 --> 00:38:53,660
+absorbance على 540 nanometer و على .. طبعا بكون
+
+502
+00:38:53,660 --> 00:38:57,150
+مسفرة على ال plant اللي ..هو التيوب رقم واحد في
+
+503
+00:38:57,150 --> 00:39:06,070
+التيوبات ال standard curve طيب من ال curve اللي
+
+504
+00:39:06,070 --> 00:39:10,930
+أنا رسمته حكينا إنه أنا بدي أجيب slope و .. اللي
+
+505
+00:39:10,930 --> 00:39:13,830
+هو عبارة عن فرق الصدات على فرق الصينة هاي ال slope
+
+506
+00:39:13,830 --> 00:39:17,290
+عبارة عن delta Y على delta X فرق الصدات على فرق
+
+507
+00:39:17,290 --> 00:39:20,850
+الصينة طيب فرق الصدات هو عبارة عن إيش يا بنات؟ هو
+
+508
+00:39:20,850 --> 00:39:24,770
+فرق عن .. فرق absorbance طب absorbance لمين؟
+
+509
+00:39:26,590 --> 00:39:30,870
+absorbance لمين؟ ال absorbance هاد لل standard
+
+510
+00:39:30,870 --> 00:39:34,650
+لإنه أنا برسم standard curve أو برسم curve جاي من
+
+511
+00:39:34,650 --> 00:39:40,010
+أنابيب ل standard تمام؟ إذا هو عبارة عن فرق ل
+
+512
+00:39:40,010 --> 00:39:45,810
+absorbance ل standardطيب فرق الصينات عبارة عن إيش؟
+
+513
+00:39:45,810 --> 00:39:49,850
+فرق ال concentration طب ال concentration هذا لمين؟
+
+514
+00:39:49,850 --> 00:39:53,890
+لل standard إذا برضه كمان فرق ال concentration لل
+
+515
+00:39:53,890 --> 00:39:57,790
+standard إذا بإمكاني أنسى delta Y على delta X و
+
+516
+00:39:57,790 --> 00:40:00,870
+أحكي إنه ال slope هو عبارة عن ال absorbance لل
+
+517
+00:40:00,870 --> 00:40:03,630
+standard، هو عبارة عن قيمة absorbance أو فرق
+
+518
+00:40:03,630 --> 00:40:04,290
+absorbance
+
+519
+00:40:13,170 --> 00:40:16,950
+طيب لو جبت factor اللي هو عبارة عن مقلوب ال slope
+
+520
+00:40:16,950 --> 00:40:23,570
+هو عبارة عن مقلوب ال slope فأصبح عنديإنه هذا ال
+
+521
+00:40:23,570 --> 00:40:28,570
+factor هو عبارة عن concentration لل standard على
+
+522
+00:40:28,570 --> 00:40:32,670
+absorbance لل standard طيب لو أنا جبت هذا ال
+
+523
+00:40:32,670 --> 00:40:37,190
+factor و ضرب .. ضربته ب .. ال absorbance لل عينة
+
+524
+00:40:37,190 --> 00:40:41,530
+المجهولة فإيش صار عندي؟ تأملوا هذا القانون جيدا،
+
+525
+00:40:41,530 --> 00:40:47,390
+مش هو Beer-Lambert Law؟أصبح احنا يعني صح انا
+
+526
+00:40:47,390 --> 00:40:51,750
+اتطبقت على أكثر من أنبوبة standard ولكن في النهاية
+
+527
+00:40:51,750 --> 00:40:57,610
+انا ما طلعت عن اطار Beer Lambert Cloud absorbance
+
+528
+00:40:57,610 --> 00:41:02,450
+لل X على ال absorbance لل standard ضرب تركيز لل
+
+529
+00:41:02,450 --> 00:41:07,910
+standardهذا ال factor في absorbance لل X يعني أصبح
+
+530
+00:41:07,910 --> 00:41:13,350
+أنا بهذه الطريقة بكون عم بطبق Beer-Lambert law بس
+
+531
+00:41:13,350 --> 00:41:19,510
+بدلالة أكثر من cube standard و ليس cube واحد تمام؟
+
+532
+00:41:19,510 --> 00:41:24,690
+هذه هي الفكرة طيب
+
+533
+00:41:24,690 --> 00:41:30,870
+خلونا بس نقارن ما بين فحص السكر و فحص ال
+
+534
+00:41:30,870 --> 00:41:35,440
+homoglobinفحص السكر كانت العينة plasma لإن ال
+
+535
+00:41:35,440 --> 00:41:39,980
+nutrients لجلوكوز و الأشياء المغذية هاي بتكون
+
+536
+00:41:39,980 --> 00:41:45,420
+موجودة في ال plasma أو ال serum فحص ال homoglobin
+
+537
+00:41:45,420 --> 00:41:48,400
+كانت العينة هو ال blood لإن أنا معنية أحصل على
+
+538
+00:41:48,400 --> 00:41:51,760
+خلايا لإن ال homoglobin موجود داخل ال red blood
+
+539
+00:41:51,760 --> 00:41:57,560
+cells فحص السكر استخدمت standard tube واحدةوماكن
+
+540
+00:41:57,560 --> 00:42:00,780
+في داعي ان هنا ارسم curve وطبقت ال beer lambert
+
+541
+00:42:00,780 --> 00:42:06,560
+law مباشرة لكن في فحص ال homoglobin انا عملت لفة
+
+542
+00:42:06,560 --> 00:42:10,440
+هي اي نعم قدتني الى ان انا في النهاية بكون عم
+
+543
+00:42:10,440 --> 00:42:15,580
+بتطبق نفس beer lambert law بس استخدمت اكثر من tube
+
+544
+00:42:15,580 --> 00:42:21,340
+standardو رسمت curve و إنتجت إنه العلاقة ما بين ال
+
+545
+00:42:21,340 --> 00:42:24,480
+absorbance و ال concentration هي linear اتأكدت
+
+546
+00:42:24,480 --> 00:42:28,800
+بنفسي و جبت ال slope فرق الصوادر على فرق الصينات
+
+547
+00:42:28,800 --> 00:42:32,670
+جبت ال factor اللي هو مقلوب لل slopeضربت هذا ال
+
+548
+00:42:32,670 --> 00:42:37,170
+factor ب ال absorbance لا ال test لعينة المريض و
+
+549
+00:42:37,170 --> 00:42:40,870
+في النهاية تفاجأت أنه أنا كنت عم بطبق قانون بير
+
+550
+00:42:40,870 --> 00:42:47,330
+لمبرتلاو بحذافيره ولكن باستخدام اكثر من tube من ال
+
+551
+00:42:47,330 --> 00:42:54,460
+standard بدلالة اكتر من tube من ال standardال ..
+
+552
+00:42:54,460 --> 00:42:58,860
+يختلف الطول الموجي اللي أنا بقرأ عليه فحص السكر عن
+
+553
+00:42:58,860 --> 00:43:04,620
+فحص ال homoglobin و أيضا تختلف الوحدة السكر وحدتها
+
+554
+00:43:04,620 --> 00:43:08,740
+milligram per deciliter و ال homoglobin وحدته
+
+555
+00:43:08,740 --> 00:43:13,550
+اجرام per deciliterيختلف ال normal range أيضا،
+
+556
+00:43:13,550 --> 00:43:20,430
+السكر يلو مستويات معينة من 70 ل110 و ال homoglobin
+
+557
+00:43:20,430 --> 00:43:24,830
+طبعا حسب العمر و حسب ال gender يختلف ال normal
+
+558
+00:43:24,830 --> 00:43:29,470
+range، تمام؟مهم جدا تربطوا الأمور هاي مع بعض لحتى
+
+559
+00:43:29,470 --> 00:43:32,770
+تكونوا فاهمين إحنا إيش بنشتغل وكيف بنفرق وعلى أي
+
+560
+00:43:32,770 --> 00:43:35,570
+أساس بنختار نوع العينة أو بنختار طول الموجي، كيف
+
+561
+00:43:35,570 --> 00:43:38,290
+بشتغل، إيش .. إيش دور ال standard، إيش دور ال
+
+562
+00:43:38,290 --> 00:43:43,830
+blank في التجربة اللي أنا بشتغلها discussion
+
+563
+00:43:45,170 --> 00:43:48,510
+mechanical sources of error أول ما تسمعوا كلمة
+
+564
+00:43:48,510 --> 00:43:52,150
+mechanical sources of error معناها مصادر ال error
+
+565
+00:43:52,150 --> 00:43:56,850
+الخلل اللي تحدث أثناء شغليه، الشغل اليدوي، الشغل
+
+566
+00:43:56,850 --> 00:44:00,210
+العملي، تمام؟ ممكن يكون في عندي by-putting error
+
+567
+00:44:01,000 --> 00:44:04,040
+طبعا هنشتغل يا بنات محلي ال by betting أنا باخد
+
+568
+00:44:04,040 --> 00:44:07,020
+volume من ال reagent على volume من العينة volume
+
+569
+00:44:07,020 --> 00:44:09,940
+من ال reagent على volume من ال standard فهذه كلها
+
+570
+00:44:09,940 --> 00:44:14,160
+باستخدام ال automatic by bet لازم أكون متمرسة أو
+
+571
+00:44:14,160 --> 00:44:18,380
+يعني مرنة إيدي منيح متدربة على ال by bet use و
+
+572
+00:44:18,380 --> 00:44:23,680
+أيضا بستخدمها بدقة و بشكل كتير منيح و صحيةأيضا
+
+573
+00:44:23,680 --> 00:44:28,080
+لازم انا اكون منتبهة و حاذرة باستخدام المحليل يعني
+
+574
+00:44:28,080 --> 00:44:30,880
+مثلا ال drug reagent اللي هتستخدموه في فحص ال
+
+575
+00:44:30,880 --> 00:44:34,660
+homoglobin هو عبارة عن reagent ده اتملم الصابون يا
+
+576
+00:44:34,660 --> 00:44:38,080
+بنات بما انه ده اتملم الصابون اذا
+
+577
+00:44:40,700 --> 00:44:44,400
+هيكون foam و ال foam هذه ممكن تعيق عملية ال by
+
+578
+00:44:44,400 --> 00:44:48,260
+beating أو تحدث خطأ في ال volume فانا لازم اكون من
+
+579
+00:44:48,260 --> 00:44:51,020
+تبه و انا بفضي في هذا المحلول افضي على الجدار و
+
+580
+00:44:51,020 --> 00:44:54,260
+شوي شوي كمان انا بستخدم خمسة امقال من هذا ال
+
+581
+00:44:54,260 --> 00:44:58,020
+reagent كمية مش بسيطة كمية كبيرة تعادة على ال tube
+
+582
+00:44:58,020 --> 00:45:05,340
+فلازم انا لما افضي هذا ال tube اكون يعني اكون
+
+583
+00:45:05,340 --> 00:45:08,960
+حاضرة انه مايكببرة ليت يوم هعلمكم طبعا كيف انا
+
+584
+00:45:08,960 --> 00:45:13,080
+بفضي و كيف باخد ال volume كيف بعمل by beating في
+
+585
+00:45:13,080 --> 00:45:17,180
+الفحص علشان تشتغلوا خطوة بخطوة واحدة واحدة و ما
+
+586
+00:45:17,180 --> 00:45:21,820
+تجلطوا use of dirty or scratches او scratched
+
+587
+00:45:21,820 --> 00:45:22,520
+cubits
+
+588
+00:45:24,930 --> 00:45:28,730
+استخدام cuvette مش clean وفيها scratches احنا
+
+589
+00:45:28,730 --> 00:45:31,650
+حكينا من شروط ال beer lambert لأو الدقيقة و
+
+590
+00:45:31,650 --> 00:45:35,430
+الصحيحة انه يكون عندي ال .. ال .. ال cuvettes
+
+591
+00:45:35,430 --> 00:45:39,250
+النبوب اللي بحط فيها ال tube و هي عبارة عن تعملي
+
+592
+00:45:39,250 --> 00:45:43,530
+sample او solution holder داخل الجهاز لازم تكون
+
+593
+00:45:43,530 --> 00:45:47,750
+clean مش فيها خالية من الشقوق من الكثور الشطوب
+
+594
+00:45:47,750 --> 00:45:55,400
+وبالتالي ماتأثر على مسار الضوءفإذا كان عندي ال
+
+595
+00:45:55,400 --> 00:46:00,760
+cuvettes مش نظيفة، مساكتها بشكل خاطئ، صار في عليها
+
+596
+00:46:00,760 --> 00:46:06,600
+من ال cotton fiber أو تلوثت، صار مشققة، مكسورة،
+
+597
+00:46:06,600 --> 00:46:10,360
+فيها خدش، هذا راح يأثر على القراءة use of
+
+598
+00:46:10,360 --> 00:46:13,100
+deteriorated reagent
+
+599
+00:46:15,970 --> 00:46:21,210
+reaction تالف تمام؟ reaction تالف فاستخدام
+
+600
+00:46:21,210 --> 00:46:26,250
+reaction تالف بالتالي هيعطيني reaction non-proper
+
+601
+00:46:26,250 --> 00:46:29,030
+reaction أو improper reaction مش ال reaction اللي
+
+602
+00:46:29,030 --> 00:46:34,470
+بديها فبالتالي نتيجة مش صحيحة أو دقيقةأيضًا كمان
+
+603
+00:46:34,470 --> 00:46:38,990
+في عندي أمور ياجب مراعاعتها أثناء إجراء الفحص زي
+
+604
+00:46:38,990 --> 00:46:43,230
+مثلًا أنه المحلول اللي أنا بشتغل عليه أو اللي بدي
+
+605
+00:46:43,230 --> 00:46:47,470
+أقيسه يكون أطائي clear صح هو ملون و أنا بدي أقيس
+
+606
+00:46:47,470 --> 00:46:51,630
+شدة لون على الجهاز بس لازم يكونمافيش فيه Akara
+
+607
+00:46:51,630 --> 00:46:54,970
+مافيش فيه precipitation يعني لو شخص عنده ال white
+
+608
+00:46:54,970 --> 00:46:59,850
+pieces عالية كتير هذه هتأدي إلى أنه المحلول اللي
+
+609
+00:46:59,850 --> 00:47:02,870
+بدي أقيصوها فيه نسبة turbidity ال white pieces
+
+610
+00:47:02,870 --> 00:47:08,760
+بتعملي turbidity زيادتهاتمام زيادة ال white bcs
+
+611
+00:47:08,760 --> 00:47:13,180
+هتعمل turbidity إذا هتأثر على ال absorbance هتأثر
+
+612
+00:47:13,180 --> 00:47:17,360
+على مصال الدوة هيزيد ال absorbance فبالتالي يبطيني
+
+613
+00:47:17,360 --> 00:47:23,870
+نسبته مجلوبين falsely elevatedHomoglobin S و
+
+614
+00:47:23,870 --> 00:47:26,990
+Homoglobin C هدول الأنواع من ال Homoglobin ..
+
+615
+00:47:26,990 --> 00:47:29,910
+Homoglobin S هو ال Homoglobin اللي موجود عند الناس
+
+616
+00:47:29,910 --> 00:47:33,630
+اللي مصابين بال Sickness الانيميا .. بانيميا
+
+617
+00:47:33,630 --> 00:47:37,950
+الخلايا المنجالية و ال Homoglobin C برضه كمان
+
+618
+00:47:37,950 --> 00:47:44,050
+بيكون عند ناس مصابين بتفرة أخرى مشابهة زي .. زي ال
+
+619
+00:47:44,050 --> 00:47:48,010
+Sickness الانيميا المحصلة انه هذا ال Homoglobin
+
+620
+00:47:48,010 --> 00:47:54,040
+لما بيتأكسد بيترسبتمام؟ homoglobin s و homoglobin
+
+621
+00:47:54,040 --> 00:47:59,680
+c طب احنا اصلا مبدأ عملنا بيعتمد
+
+622
+00:47:59,680 --> 00:48:02,840
+على تأكسد ال homoglobin على أكسدة ال homoglobin
+
+623
+00:48:02,840 --> 00:48:06,480
+يتحول من homoglobin إلى met homoglobin فتخيل
+
+624
+00:48:06,480 --> 00:48:09,700
+homoglobin s يتحول ل met homoglobin s او
+
+625
+00:48:09,700 --> 00:48:13,960
+homoglobin c يتحول ل met homoglobin c معناه هيترسب
+
+626
+00:48:14,530 --> 00:48:17,250
+طب إحنا مش حكينا إنه من شروط ال dilution not
+
+627
+00:48:17,250 --> 00:48:21,270
+associate or dissociate مايترسبش مايكونش فيه عكارة
+
+628
+00:48:21,270 --> 00:48:24,770
+مايأثرش أنا بدي أقيس بس اللون اللي جاي من المادة
+
+629
+00:48:24,770 --> 00:48:30,390
+الملونة اللي هي cyanomethamoglobin تمام فعشان هيك
+
+630
+00:48:30,390 --> 00:48:35,650
+هذا عشان ألغي تأثير هذا الراسب عن نتيجة ب .. بعمل
+
+631
+00:48:35,650 --> 00:48:39,730
+dilution للعينة باستخدام distilled water أو saline
+
+632
+00:48:39,730 --> 00:48:45,060
+بنسبة 1 إلى 1 يعني ال dilution هيكون نص تمام؟فإذا
+
+633
+00:48:45,060 --> 00:48:51,160
+يعني باخد حجم من العينة وحجم equal مساوي من ال
+
+634
+00:48:51,160 --> 00:48:54,820
+distilled water يعني بنسبة واحد إلى واحد ال total
+
+635
+00:48:54,820 --> 00:48:58,860
+dilution هيكون واحد لواحد ده ايه؟ زائد واحد يعني
+
+636
+00:48:58,860 --> 00:49:05,860
+واحد لنص واحد لاتنين sorry تمام نص فبالتاليال ..
+
+637
+00:49:05,860 --> 00:49:09,020
+ال .. النتيجة تبعتي لما بتطلع أكيد بدي أضربها بال
+
+638
+00:49:09,020 --> 00:49:12,000
+dilution factor اللي هو 2 عشان أحصل على ال
+
+639
+00:49:12,000 --> 00:49:15,820
+original concentration راجعوا ال dilution لأن دوها
+
+640
+00:49:15,820 --> 00:49:20,760
+يكونوا لاحظين إنه كل مرة في كل محاضرة أو كل ما
+
+641
+00:49:20,760 --> 00:49:24,500
+بنتقدم شوية ال dilution بيضل مرافقنا، بيضل معنا،
+
+642
+00:49:24,500 --> 00:49:27,760
+فالمنيح إنه تكونوا مراجعينه وحافظينه ومتمكنين منه
+
+643
+00:49:28,430 --> 00:49:33,290
+Libemia إيش يعني ليبياميا؟ ليبي يعني جاي من لبد،
+
+644
+00:49:33,290 --> 00:49:37,570
+دهون، ايميا حاكينا في الدم، إذا زيادة الدهون في
+
+645
+00:49:37,570 --> 00:49:41,990
+الدم بسميها ليبياميا طبعا الدهون في الدم، الدهون
+
+646
+00:49:41,990 --> 00:49:47,730
+تحت عكارة أصلا يعني، فالحالة هذه ممكن تعطيني false
+
+647
+00:49:47,730 --> 00:49:52,520
+resultفي وجود نسبة .. نسبة عالية من الدهون في الدم
+
+648
+00:49:52,520 --> 00:49:58,020
+فبالتالي يفضل إن أنا الـ Drapkin reagent الـ Blank
+
+649
+00:49:58,020 --> 00:50:03,630
+عينة الـ Blank تبعتيأضيف عليها عشرين microliter
+
+650
+00:50:03,630 --> 00:50:08,570
+اللي هو point zero two ml من البلازمة طب ما هي
+
+651
+00:50:08,570 --> 00:50:11,750
+البلازمة إيش؟ عبارة عن إيش؟ عبارة عن ما موجود فيها
+
+652
+00:50:11,750 --> 00:50:16,250
+الدهون تمام؟ وروح أصفر عليها طب لما أصفر على ال
+
+653
+00:50:16,250 --> 00:50:18,950
+reagent هذا اللي أنا أضفتله عشرين microliter من
+
+654
+00:50:18,950 --> 00:50:23,770
+البلازمة إيش أنا استفدت؟ أنا بكون لغاية اللون اللي
+
+655
+00:50:23,770 --> 00:50:27,270
+جاي من ال reagent و لغاية اللون اللي جاي من
+
+656
+00:50:27,270 --> 00:50:31,590
+البلازمة اللي هو من الدهون اللي في البلازمةلغاية
+
+657
+00:50:31,590 --> 00:50:35,910
+تأثير الدهون اللى موجودة تربدت اللى عاملها الدهون
+
+658
+00:50:35,910 --> 00:50:43,490
+على نتيجة فحصه تمام ال drug in reagent is
+
+659
+00:50:43,490 --> 00:50:47,870
+sensitive to light هذا ال reagent حساس للضوء لازم
+
+660
+00:50:47,870 --> 00:50:51,530
+انه مانعرضه للضوء فبالتالي انا بحطه في ال brown
+
+661
+00:50:51,530 --> 00:50:56,740
+bottle دايما احنا بنخزنههو بيجينا مركز فبنخففه و
+
+662
+00:50:56,740 --> 00:51:01,620
+بحضر منه working solution بخزنه في قنابيب dark
+
+663
+00:51:01,620 --> 00:51:07,580
+لونها brown و بحطها أيضا في أماكن معتمة بعيدة عن
+
+664
+00:51:07,580 --> 00:51:11,540
+الضوء علشان مايتكسر هذا ال reagent مايصيرش
+
+665
+00:51:11,540 --> 00:51:14,940
+deteriorated لإن إذا صار deteriorated يعني في عندي
+
+666
+00:51:14,940 --> 00:51:19,840
+errorطيب carboxy-homoglobin الناس اللي عندها نسبة
+
+667
+00:51:19,840 --> 00:51:23,340
+لها carboxy-homoglobin عالية زي المدخنين فهدول
+
+668
+00:51:23,340 --> 00:51:27,880
+بيحتاج ال .. ال homoglobin هذا ساعة واحدة عشان
+
+669
+00:51:27,880 --> 00:51:32,260
+يتكون ساعة مش عشر دقائق ال incubation period ساعة
+
+670
+00:51:32,260 --> 00:51:36,840
+عشان يتحول لسيانومة homoglobin فهذا طبعا لو انا ..
+
+671
+00:51:36,840 --> 00:51:39,420
+لو ماكنتش عارفة ان الشخص هذا مدخن يعني لأنه عنده
+
+672
+00:51:39,420 --> 00:51:42,840
+carboxy-homoglobin اذا و قرأت خلال عشر دقائق
+
+673
+00:51:45,910 --> 00:51:49,050
+يعني أنا مستهلكتش ساعة كاملة عشان يتحول كل
+
+674
+00:51:49,050 --> 00:51:52,370
+الكربوكسيهوموجلوبين لسيانو متهموجلوبين، إذا هقرأ
+
+675
+00:51:52,370 --> 00:51:56,250
+نتي أنه مش كل الهموجلوبين اتحول فبالتالي ممكن يكون
+
+676
+00:51:56,250 --> 00:52:00,290
+في عندي error بس بحكيلك أنه أحيانا ال degree of
+
+677
+00:52:00,290 --> 00:52:03,350
+error هذه، نسبة ال error هذه بتكونش واضحة كتير،
+
+678
+00:52:03,350 --> 00:52:07,710
+ليش؟ لإنه احنا ذكرنا في بداية المحاضرة أنه ال
+
+679
+00:52:07,710 --> 00:52:12,490
+smokers هدول عندهم نسبة متهموجلوبين عندهم دم أكسد
+
+680
+00:52:12,490 --> 00:52:17,910
+وزاق جاهزتمام؟ فهذا بعادل النسبة، بالعكس أحيانًا
+
+681
+00:52:17,910 --> 00:52:22,030
+بتكون نسبتهم عالية، كمان؟ because the drug kidney
+
+682
+00:52:22,030 --> 00:52:25,390
+agent contains cyanide لإنه بيحتوي على ال cyanide
+
+683
+00:52:25,390 --> 00:52:31,290
+المادة هذه ثامة، خانقة، بتأدي إلى توقف أو
+
+684
+00:52:31,290 --> 00:52:36,670
+inhibition لإنزيمات ال glycolysis و ال creep cycle
+
+685
+00:52:36,670 --> 00:52:41,810
+لإنزيمات التنفس الخلوي، عمليات الايد والميتابوليزم
+
+686
+00:52:41,810 --> 00:52:47,390
+اللي موجودة في الخلاياتمام؟ فبالتالي سامة و قاتلة
+
+687
+00:52:47,390 --> 00:52:50,630
+و طبعا irreversible يا بنات بتعمل inhibition
+
+688
+00:52:50,630 --> 00:52:54,730
+irreversible و لعلكم أخدتوا الإنزيمات و ال
+
+689
+00:52:54,730 --> 00:52:58,370
+inhibitors و النوع ال reversible و ال irreversible
+
+690
+00:52:58,370 --> 00:53:01,470
+تبعها فال cyanide أحد ال irreversible inhibitors
+
+691
+00:53:01,470 --> 00:53:06,750
+لل cell respiratory enzymes تمام؟ و إذا هيقدر إلى
+
+692
+00:53:06,750 --> 00:53:09,790
+اختناق فقتل يعني أربعة liter من هذا ال reagent
+
+693
+00:53:09,790 --> 00:53:14,750
+ممكن تكون قاتلة تناولهافبالتالي ناخد حضارنا منه
+
+694
+00:53:14,750 --> 00:53:17,810
+ايضا الى انه ممكن انها تعمل irritation ل ال skin
+
+695
+00:53:17,810 --> 00:53:23,010
+مش لازم يجي على ال ايدي فلازم انا اكون بستخدم يعني
+
+696
+00:53:23,010 --> 00:53:29,290
+لابسة ل gloves لابسة ال lab coat تبعي يعني دايرة
+
+697
+00:53:29,290 --> 00:53:32,650
+بالي احلي ملتزمة بكل الإجراءات السلامة قبل البدء
+
+698
+00:53:32,650 --> 00:53:36,590
+في العمل acid-free sinks should be used for
+
+699
+00:53:36,590 --> 00:53:44,720
+disposal of this reagent لازم انا استخدمأو أتخلص
+
+700
+00:53:44,720 --> 00:53:49,780
+من هذا ال reagent بأحواض يعني الحوض أو ال .. ال
+
+701
+00:53:49,780 --> 00:53:54,480
+sink .. يعني الحوض الحنفية أنه يكون هذا لا .. يعني
+
+702
+00:53:54,480 --> 00:53:57,900
+أنا مابسكبش فيه acids يعني مابيتنضفش ب acids أو
+
+703
+00:53:57,900 --> 00:54:02,580
+مابسكبش فيه acidsليش؟ لإنه إذا أنا كبيت المحلول
+
+704
+00:54:02,580 --> 00:54:05,280
+اللي هو فيه cyanide وبعدين جيت روحت مضفت بchlor أو
+
+705
+00:54:05,280 --> 00:54:09,720
+ب acid أو كبيت acid فمعناه ال acid هيتراح يروح
+
+706
+00:54:09,720 --> 00:54:12,440
+يتفاعل مع ال cyanide و هيكوملي ال hydrogen cyanide
+
+707
+00:54:12,440 --> 00:54:21,020
+gas اللي هو عبارة عن toxic gas، تمام؟ غاز سامفيفضل
+
+708
+00:54:21,020 --> 00:54:24,480
+انه انا كمان بعد ما انا اتخلص مثلا الانابيب
+
+709
+00:54:24,480 --> 00:54:27,660
+تابعتنا اشتغلنا فيها خلصناها مينفع .. يعني انبوب
+
+710
+00:54:27,660 --> 00:54:30,420
+مليان فيه خمس أمقال من ال reagent يعني كمية اللي
+
+711
+00:54:30,420 --> 00:54:33,760
+بقصبها مينفعش انا اكبها بالسلة فبالتالي بروح انا
+
+712
+00:54:33,760 --> 00:54:37,560
+بطلق .. بكب محتوها بالحوت بعدين بتخلص من الانابيب
+
+713
+00:54:37,560 --> 00:54:43,620
+فإذا ..لازم انه انا افتح الحنفية شوي اسيب من الماء
+
+714
+00:54:43,620 --> 00:54:46,740
+الجارى بحيث انه يعمللي dilution لهذا ال reagent
+
+715
+00:54:46,740 --> 00:54:50,960
+انه لو فيما بعد تم إطلاف acid او اتنظف الحوت
+
+716
+00:54:50,960 --> 00:54:56,240
+بchlor او اشى مايروح يتفاعل ال cyanide مع ال acid
+
+717
+00:54:56,240 --> 00:54:59,040
+و يكونلي hydrogen cyanide gas اللى هو toxic gas
+
+718
+00:54:59,570 --> 00:55:03,050
+تمام؟ إذا هتلاقوني هتلاحظوا عليا إنه أول ما بدتوا
+
+719
+00:55:03,050 --> 00:55:06,390
+تطلفوا أو إنه تعملوا discard لمحتوى القنابيب
+
+720
+00:55:06,390 --> 00:55:10,270
+تلاقوني فاتحت الحنافية و سبتها مفتوحة ماحدش يسكرها
+
+721
+00:55:10,270 --> 00:55:14,290
+لإنها بديها تعمل dilution لها هذا ال agent بحيث
+
+722
+00:55:14,290 --> 00:55:20,770
+إنه ما يكون عم بنشكل أو بننتج غاز سام إنه بعد هيك
+
+723
+00:55:20,770 --> 00:55:26,140
+أي حد يفوت على المختبر يتأذىهك بنكون انتهانا من
+
+724
+00:55:26,140 --> 00:55:29,700
+محاضرتنا لليوم يعطيكم ألف عافية ونراكم في المعمل
+
+725
+00:55:29,700 --> 00:55:30,360
+للتطبيق
+
diff --git a/PL9fwy3NUQKwY9jl8xw-bWkVZkWcGGyspd/ZZ8KPmQPnAc_raw.json b/PL9fwy3NUQKwY9jl8xw-bWkVZkWcGGyspd/ZZ8KPmQPnAc_raw.json
new file mode 100644
index 0000000000000000000000000000000000000000..9834e20c25e062120c2cd214119130dc4111a603
--- /dev/null
+++ b/PL9fwy3NUQKwY9jl8xw-bWkVZkWcGGyspd/ZZ8KPmQPnAc_raw.json
@@ -0,0 +1 @@
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"end": 80.67, "word": " blood", "probability": 0.94677734375}, {"start": 80.67, "end": 81.53, "word": " cells،", "probability": 0.7578125}, {"start": 81.53, "end": 82.13, "word": " تمام؟", "probability": 0.9889322916666666}, {"start": 82.13, "end": 82.37, "word": " من", "probability": 0.990234375}, {"start": 82.37, "end": 83.21, "word": " لونها،", "probability": 0.9169921875}, {"start": 83.21, "end": 83.39, "word": " من", "probability": 0.9951171875}, {"start": 83.39, "end": 83.71, "word": " لون", "probability": 0.98291015625}, {"start": 83.71, "end": 84.03, "word": " ال", "probability": 0.96923828125}, {"start": 84.03, "end": 84.63, "word": " homoglobin", "probability": 0.8408203125}, {"start": 84.63, "end": 85.09, "word": " فيها", "probability": 0.99462890625}], "temperature": 1.0}, {"id": 4, "seek": 11161, "start": 85.33, "end": 111.61, "text": "لأن هذا ال homoglobin هو ال breed dominant protein هو ال protein الأكثر شيوعا و المحتل أكبر حجم من خلايا ال RBCs أكثر من 60% من حجم ال RBCs هو عبارة عن homoglobin تمام؟ هو عبارة عن homoglobin homoglobin carries oxygen from places of high oxygen pressure lungs", "tokens": [1211, 33456, 23758, 2423, 3655, 45841, 13496, 31439, 2423, 18971, 15657, 7944, 31439, 2423, 7944, 16247, 4117, 49115, 13412, 1829, 45367, 995, 4032, 9673, 33753, 1211, 5551, 4117, 26890, 11331, 7435, 2304, 9154, 16490, 15040, 25528, 2423, 497, 7869, 82, 5551, 4117, 49115, 9154, 4060, 4, 9154, 11331, 7435, 2304, 2423, 497, 7869, 82, 31439, 6225, 3555, 9640, 3660, 18871, 3655, 45841, 13496, 46811, 10943, 22807, 31439, 6225, 3555, 9640, 3660, 18871, 3655, 45841, 13496, 3655, 45841, 13496, 16402, 9169, 490, 3190, 295, 1090, 9169, 3321, 19467], "avg_logprob": -0.13831676712090318, "compression_ratio": 1.674757281553398, "no_speech_prob": 5.960464477539063e-08, "words": [{"start": 85.33, "end": 85.91, "word": "لأن", "probability": 0.842041015625}, {"start": 85.91, "end": 86.51, "word": " هذا", "probability": 0.8037109375}, {"start": 86.51, "end": 86.67, "word": " ال", "probability": 0.9931640625}, {"start": 86.67, "end": 87.41, "word": " homoglobin", "probability": 0.6063639322916666}, {"start": 87.41, "end": 87.97, "word": " هو", "probability": 0.986328125}, {"start": 87.97, "end": 88.27, "word": " ال", "probability": 0.947265625}, {"start": 88.27, "end": 88.69, "word": " breed", "probability": 0.30615234375}, {"start": 88.69, "end": 89.39, "word": " dominant", "probability": 0.91943359375}, {"start": 89.39, "end": 90.09, "word": " protein", "probability": 0.45361328125}, {"start": 90.09, "end": 90.37, "word": " هو", "probability": 0.8076171875}, {"start": 90.37, "end": 90.55, "word": " ال", "probability": 0.951171875}, {"start": 90.55, "end": 90.95, "word": " protein", "probability": 0.81494140625}, {"start": 90.95, "end": 91.79, "word": " الأكثر", "probability": 0.9430338541666666}, {"start": 91.79, "end": 92.53, "word": " شيوعا", "probability": 0.8895263671875}, {"start": 92.53, "end": 92.71, "word": " و", "probability": 0.9228515625}, {"start": 92.71, "end": 93.47, "word": " المحتل", "probability": 0.7957356770833334}, {"start": 93.47, "end": 94.13, "word": " أكبر", "probability": 0.9654947916666666}, {"start": 94.13, "end": 95.03, "word": " حجم", "probability": 0.986328125}, {"start": 95.03, "end": 95.29, "word": " من", "probability": 0.9912109375}, {"start": 95.29, "end": 95.75, "word": " خلايا", "probability": 0.9762369791666666}, {"start": 95.75, "end": 95.87, "word": " ال", "probability": 0.93408203125}, {"start": 95.87, "end": 96.43, "word": " RBCs", "probability": 0.7952473958333334}, {"start": 96.43, "end": 97.01, "word": " أكثر", "probability": 0.9143880208333334}, {"start": 97.01, "end": 97.21, "word": " من", "probability": 0.98681640625}, {"start": 97.21, "end": 97.61, "word": " 60", "probability": 0.92578125}, {"start": 97.61, "end": 98.03, "word": "%", "probability": 0.96728515625}, {"start": 98.03, "end": 98.37, "word": " من", "probability": 0.99072265625}, {"start": 98.37, "end": 98.85, "word": " حجم", "probability": 0.99609375}, {"start": 98.85, "end": 99.01, "word": " ال", "probability": 0.93896484375}, {"start": 99.01, "end": 100.19, "word": " RBCs", "probability": 0.96484375}, {"start": 100.19, "end": 100.55, "word": " هو", "probability": 0.78125}, {"start": 100.55, "end": 101.37, "word": " عبارة", "probability": 0.998046875}, {"start": 101.37, "end": 101.75, "word": " عن", "probability": 0.9931640625}, {"start": 101.75, "end": 102.81, "word": " homoglobin", "probability": 0.8986002604166666}, {"start": 102.81, "end": 104.25, "word": " تمام؟", "probability": 0.8006184895833334}, {"start": 104.25, "end": 104.49, "word": " هو", "probability": 0.9267578125}, {"start": 104.49, "end": 104.95, "word": " عبارة", "probability": 0.9989013671875}, {"start": 104.95, "end": 105.09, "word": " عن", "probability": 0.9931640625}, {"start": 105.09, "end": 105.73, "word": " homoglobin", "probability": 0.9129231770833334}, {"start": 105.73, "end": 107.13, "word": " homoglobin", "probability": 0.8898111979166666}, {"start": 107.13, "end": 107.65, "word": " carries", "probability": 0.853515625}, {"start": 107.65, "end": 108.31, "word": " oxygen", "probability": 0.79052734375}, {"start": 108.31, "end": 108.69, "word": " from", "probability": 0.923828125}, {"start": 108.69, "end": 109.25, "word": " places", "probability": 0.923828125}, {"start": 109.25, "end": 109.57, "word": " of", "probability": 0.96240234375}, {"start": 109.57, "end": 110.23, "word": " high", "probability": 0.91162109375}, {"start": 110.23, "end": 110.75, "word": " oxygen", "probability": 0.83349609375}, {"start": 110.75, "end": 111.17, "word": " pressure", "probability": 0.95654296875}, {"start": 111.17, "end": 111.61, "word": " lungs", "probability": 0.576171875}], "temperature": 1.0}, {"id": 5, "seek": 13722, "start": 112.32, "end": 137.22, "text": "to places of low oxygen pressure such as tissues ال homoglobin حكينا إيش وظيفته؟ هو عبارة عن ناقل لأل أكسوجين، إذا هو أثناء سرايان الدم و يذهبوا لال lungs، للريقتين، في الريقتين موجود ضغط عالي من الأكسوجين، تمام؟ لإن الريقتين هي اللي بتستلم الأكسوجين، نعمل إحنا تنفس، شاهيك", "tokens": [1353, 3190, 295, 2295, 9169, 3321, 1270, 382, 27353, 2423, 3655, 45841, 13496, 11331, 4117, 1829, 8315, 11933, 1829, 8592, 4032, 19913, 33911, 47395, 22807, 31439, 6225, 3555, 9640, 3660, 18871, 8717, 995, 4587, 1211, 5296, 10721, 1211, 5551, 4117, 3794, 29245, 9957, 12399, 11933, 15730, 31439, 5551, 12984, 1863, 16606, 8608, 23557, 1829, 7649, 32748, 2304, 4032, 7251, 24192, 3555, 14407, 5296, 6027, 19467, 12399, 24976, 16572, 38149, 9957, 12399, 8978, 2423, 16572, 38149, 9957, 3714, 29245, 23328, 48812, 17082, 9566, 6225, 6027, 1829, 9154, 16247, 4117, 3794, 29245, 9957, 12399, 46811, 10943, 22807, 5296, 28814, 1863, 2423, 16572, 38149, 9957, 39896, 13672, 1829, 39894, 14851, 19528, 16247, 4117, 3794, 29245, 9957, 12399, 8717, 25957, 1211, 11933, 5016, 8315, 6055, 1863, 36178, 12399, 13412, 40294, 1829, 4117], "avg_logprob": -0.17902131782945738, "compression_ratio": 1.6567164179104477, "no_speech_prob": 1.430511474609375e-06, "words": [{"start": 112.32, "end": 112.6, "word": "to", "probability": 0.89599609375}, {"start": 112.6, "end": 113.24, "word": " places", "probability": 0.9267578125}, {"start": 113.24, "end": 113.58, "word": " of", "probability": 0.9736328125}, {"start": 113.58, "end": 114.16, "word": " low", "probability": 0.95654296875}, {"start": 114.16, "end": 114.66, "word": " oxygen", "probability": 0.8330078125}, {"start": 114.66, "end": 115.1, "word": " pressure", "probability": 0.7822265625}, {"start": 115.1, "end": 115.44, "word": " such", "probability": 0.908203125}, {"start": 115.44, "end": 115.84, "word": " as", "probability": 0.97900390625}, {"start": 115.84, "end": 116.28, "word": " tissues", "probability": 0.9716796875}, {"start": 116.28, "end": 117.04, "word": " ال", "probability": 0.80859375}, {"start": 117.04, "end": 117.66, "word": " homoglobin", "probability": 0.71435546875}, {"start": 117.66, "end": 118.3, "word": " حكينا", "probability": 0.8782958984375}, {"start": 118.3, "end": 118.52, "word": " إيش", "probability": 0.7704264322916666}, {"start": 118.52, "end": 119.22, "word": " وظيفته؟", "probability": 0.92705078125}, {"start": 119.22, "end": 119.42, "word": " هو", "probability": 0.96630859375}, {"start": 119.42, "end": 120.2, "word": " عبارة", "probability": 0.9957275390625}, {"start": 120.2, "end": 120.54, "word": " عن", "probability": 0.994140625}, {"start": 120.54, "end": 121.56, "word": " ناقل", "probability": 0.9722900390625}, {"start": 121.56, "end": 122.16, "word": " لأل", "probability": 0.5232340494791666}, {"start": 122.16, "end": 123.24, "word": " أكسوجين،", "probability": 0.7842610677083334}, {"start": 123.24, "end": 123.46, "word": " إذا", "probability": 0.760498046875}, {"start": 123.46, "end": 123.8, "word": " هو", "probability": 0.93994140625}, {"start": 123.8, "end": 124.76, "word": " أثناء", "probability": 0.978515625}, {"start": 124.76, "end": 125.4, "word": " سرايان", "probability": 0.82830810546875}, {"start": 125.4, "end": 125.88, "word": " الدم", "probability": 0.960205078125}, {"start": 125.88, "end": 126.18, "word": " و", "probability": 0.9384765625}, {"start": 126.18, "end": 127.4, "word": " يذهبوا", "probability": 0.71173095703125}, {"start": 127.4, "end": 128.0, "word": " لال", "probability": 0.6331787109375}, {"start": 128.0, "end": 128.56, "word": " lungs،", "probability": 0.734619140625}, {"start": 128.56, "end": 129.74, "word": " للريقتين،", "probability": 0.77490234375}, {"start": 129.74, "end": 129.84, "word": " في", "probability": 0.71826171875}, {"start": 129.84, "end": 130.42, "word": " الريقتين", "probability": 0.9395751953125}, {"start": 130.42, "end": 130.94, "word": " موجود", "probability": 0.99267578125}, {"start": 130.94, "end": 131.44, "word": " ضغط", "probability": 0.9744466145833334}, {"start": 131.44, "end": 131.94, "word": " عالي", "probability": 0.9879557291666666}, {"start": 131.94, "end": 132.08, "word": " من", "probability": 0.99365234375}, {"start": 132.08, "end": 132.9, "word": " الأكسوجين،", "probability": 0.8681640625}, {"start": 132.9, "end": 133.34, "word": " تمام؟", "probability": 0.9440104166666666}, {"start": 133.34, "end": 133.58, "word": " لإن", "probability": 0.9080403645833334}, {"start": 133.58, "end": 133.98, "word": " الريقتين", "probability": 0.9390869140625}, {"start": 133.98, "end": 134.16, "word": " هي", "probability": 0.9208984375}, {"start": 134.16, "end": 134.34, "word": " اللي", "probability": 0.944091796875}, {"start": 134.34, "end": 135.16, "word": " بتستلم", "probability": 0.7645670572916666}, {"start": 135.16, "end": 135.68, "word": " الأكسوجين،", "probability": 0.9160970052083334}, {"start": 135.68, "end": 135.92, "word": " نعمل", "probability": 0.97998046875}, {"start": 135.92, "end": 136.14, "word": " إحنا", "probability": 0.8377278645833334}, {"start": 136.14, "end": 136.72, "word": " تنفس،", "probability": 0.775390625}, {"start": 136.72, "end": 137.22, "word": " شاهيك", "probability": 0.6123046875}], "temperature": 1.0}, {"id": 6, "seek": 16034, "start": 137.6, "end": 160.34, "text": "الأكسوجين بيدخل إلى الرئتين، اذاً الرئتين فيها high oxygen pressure ساعتها الهمoglobin لما يكون عند الرئتين أو يكون في الرئتين إيش راح يعمل؟ راح يبدأ يحمل أكسوجين، تمام؟ الهمoglobin راح يحمل أكسوجين في الرئتين ولمّا يعمل circulation هذا الهمoglobin المحمل بالأكسوجين", "tokens": [6027, 10721, 4117, 3794, 29245, 9957, 4724, 25708, 9778, 1211, 30731, 34892, 19986, 2655, 9957, 12399, 1975, 15730, 14111, 34892, 19986, 2655, 9957, 8978, 11296, 1090, 9169, 3321, 8608, 995, 34268, 11296, 2423, 16095, 45841, 13496, 5296, 15042, 7251, 30544, 43242, 34892, 19986, 2655, 9957, 34051, 7251, 30544, 8978, 34892, 19986, 2655, 9957, 11933, 1829, 8592, 12602, 39319, 7251, 25957, 1211, 22807, 12602, 39319, 7251, 44510, 10721, 7251, 35571, 1211, 5551, 4117, 3794, 29245, 9957, 12399, 46811, 10943, 22807, 2423, 16095, 45841, 13496, 12602, 39319, 7251, 35571, 1211, 5551, 4117, 3794, 29245, 9957, 8978, 34892, 19986, 2655, 9957, 4032, 19528, 11703, 995, 7251, 25957, 1211, 23168, 23758, 2423, 16095, 45841, 13496, 9673, 35571, 1211, 20666, 10721, 4117, 3794, 29245, 9957], "avg_logprob": -0.145919426413607, "compression_ratio": 2.0182648401826486, "no_speech_prob": 3.039836883544922e-06, "words": [{"start": 137.6, "end": 138.18, "word": "الأكسوجين", "probability": 0.79693603515625}, {"start": 138.18, "end": 138.68, "word": " بيدخل", "probability": 0.9508056640625}, {"start": 138.68, "end": 138.84, "word": " إلى", "probability": 0.7626953125}, {"start": 138.84, "end": 139.82, "word": " الرئتين،", "probability": 0.692822265625}, {"start": 139.82, "end": 140.1, "word": " اذاً", "probability": 0.5537109375}, {"start": 140.1, "end": 140.5, "word": " الرئتين", "probability": 0.9644775390625}, {"start": 140.5, "end": 140.84, "word": " فيها", "probability": 0.9599609375}, {"start": 140.84, "end": 141.38, "word": " high", "probability": 0.96142578125}, {"start": 141.38, "end": 141.84, "word": " oxygen", "probability": 0.90966796875}, {"start": 141.84, "end": 142.38, "word": " pressure", "probability": 0.94677734375}, {"start": 142.38, "end": 143.44, "word": " ساعتها", "probability": 0.9095458984375}, {"start": 143.44, "end": 144.08, "word": " الهمoglobin", "probability": 0.68115234375}, {"start": 144.08, "end": 144.36, "word": " لما", "probability": 0.94482421875}, {"start": 144.36, "end": 144.68, "word": " يكون", "probability": 0.9912109375}, {"start": 144.68, "end": 144.92, "word": " عند", "probability": 0.98486328125}, {"start": 144.92, "end": 145.54, "word": " الرئتين", "probability": 0.987060546875}, {"start": 145.54, "end": 145.74, "word": " أو", "probability": 0.669921875}, {"start": 145.74, "end": 146.0, "word": " يكون", "probability": 0.766845703125}, {"start": 146.0, "end": 146.12, "word": " في", "probability": 0.900390625}, {"start": 146.12, "end": 146.48, "word": " الرئتين", "probability": 0.9857177734375}, {"start": 146.48, "end": 146.92, "word": " إيش", "probability": 0.7170817057291666}, {"start": 146.92, "end": 147.08, "word": " راح", "probability": 0.80419921875}, {"start": 147.08, "end": 148.1, "word": " يعمل؟", "probability": 0.974609375}, {"start": 148.1, "end": 148.34, "word": " راح", "probability": 0.936279296875}, {"start": 148.34, "end": 148.76, "word": " يبدأ", "probability": 0.9860026041666666}, {"start": 148.76, "end": 149.78, "word": " يحمل", "probability": 0.9327799479166666}, {"start": 149.78, "end": 151.0, "word": " أكسوجين،", "probability": 0.90966796875}, {"start": 151.0, "end": 151.72, "word": " تمام؟", "probability": 0.9200846354166666}, {"start": 151.72, "end": 152.48, "word": " الهمoglobin", "probability": 0.933837890625}, {"start": 152.48, "end": 152.74, "word": " راح", "probability": 0.953125}, {"start": 152.74, "end": 153.38, "word": " يحمل", "probability": 0.97265625}, {"start": 153.38, "end": 153.96, "word": " أكسوجين", "probability": 0.97724609375}, {"start": 153.96, "end": 154.12, "word": " في", "probability": 0.98974609375}, {"start": 154.12, "end": 154.8, "word": " الرئتين", "probability": 0.98974609375}, {"start": 154.8, "end": 156.02, "word": " ولمّا", "probability": 0.774169921875}, {"start": 156.02, "end": 156.56, "word": " يعمل", "probability": 0.9724934895833334}, {"start": 156.56, "end": 157.42, "word": " circulation", "probability": 0.9462890625}, {"start": 157.42, "end": 157.9, "word": " هذا", "probability": 0.85302734375}, {"start": 157.9, "end": 158.58, "word": " الهمoglobin", "probability": 0.9393310546875}, {"start": 158.58, "end": 159.5, "word": " المحمل", "probability": 0.9283854166666666}, {"start": 159.5, "end": 160.34, "word": " بالأكسوجين", "probability": 0.985595703125}], "temperature": 1.0}, {"id": 7, "seek": 18500, "start": 160.58, "end": 185.0, "text": "و يصل ال tissue أو ال organs أعضاء الجسم أو أنسجة الجسم اللى عندها low oxygen pressure مافيش عندها oxygen كفاية اش بيروح بيعمل بيبدأ يترك هذا ال oxygen اللى حمله إذا ال homoglobin هي هيك خواص عمله في ال high oxygen pressure بيبدأ يستلم يحمل oxygen تمام؟", "tokens": [2407, 7251, 36520, 2423, 12404, 34051, 2423, 20659, 5551, 3615, 11242, 16606, 25724, 38251, 34051, 14739, 3794, 7435, 3660, 25724, 38251, 13672, 7578, 43242, 11296, 2295, 9169, 3321, 19446, 41185, 8592, 43242, 11296, 9169, 9122, 5172, 995, 10632, 1975, 8592, 4724, 13546, 2407, 5016, 4724, 1829, 25957, 1211, 4724, 1829, 44510, 10721, 7251, 2655, 31747, 23758, 2423, 9169, 13672, 7578, 11331, 42213, 3224, 11933, 15730, 2423, 3655, 45841, 13496, 39896, 39896, 4117, 16490, 2407, 33546, 6225, 42213, 3224, 8978, 2423, 1090, 9169, 3321, 4724, 1829, 44510, 10721, 7251, 14851, 19528, 7251, 35571, 1211, 9169, 46811, 10943, 22807], "avg_logprob": -0.14014667819957344, "compression_ratio": 1.7454545454545454, "no_speech_prob": 5.960464477539063e-08, "words": [{"start": 160.58, "end": 160.84, "word": "و", "probability": 0.8125}, {"start": 160.84, "end": 161.18, "word": " يصل", "probability": 0.860107421875}, {"start": 161.18, "end": 161.34, "word": " ال", "probability": 0.54541015625}, {"start": 161.34, "end": 161.62, "word": " tissue", "probability": 0.78173828125}, {"start": 161.62, "end": 162.04, "word": " أو", "probability": 0.6953125}, {"start": 162.04, "end": 162.26, "word": " ال", "probability": 0.94580078125}, {"start": 162.26, "end": 162.96, "word": " organs", "probability": 0.98974609375}, {"start": 162.96, "end": 164.42, "word": " أعضاء", "probability": 0.8819580078125}, {"start": 164.42, "end": 164.82, "word": " الجسم", "probability": 0.990478515625}, {"start": 164.82, "end": 165.02, "word": " أو", "probability": 0.82763671875}, {"start": 165.02, "end": 165.6, "word": " أنسجة", "probability": 0.9197998046875}, {"start": 165.6, "end": 166.1, "word": " الجسم", "probability": 0.989013671875}, {"start": 166.1, "end": 166.92, "word": " اللى", "probability": 0.862548828125}, {"start": 166.92, "end": 167.46, "word": " عندها", "probability": 0.9931640625}, {"start": 167.46, "end": 168.02, "word": " low", "probability": 0.60009765625}, {"start": 168.02, "end": 168.7, "word": " oxygen", "probability": 0.833984375}, {"start": 168.7, "end": 169.3, "word": " pressure", "probability": 0.94140625}, {"start": 169.3, "end": 169.72, "word": " مافيش", "probability": 0.76953125}, {"start": 169.72, "end": 170.04, "word": " عندها", "probability": 0.981201171875}, {"start": 170.04, "end": 170.44, "word": " oxygen", "probability": 0.79833984375}, {"start": 170.44, "end": 170.84, "word": " كفاية", "probability": 0.973388671875}, {"start": 170.84, "end": 171.02, "word": " اش", "probability": 0.646728515625}, {"start": 171.02, "end": 171.28, "word": " بيروح", "probability": 0.96240234375}, {"start": 171.28, "end": 171.9, "word": " بيعمل", "probability": 0.926513671875}, {"start": 171.9, "end": 172.72, "word": " بيبدأ", "probability": 0.8804931640625}, {"start": 172.72, "end": 173.28, "word": " يترك", "probability": 0.9817708333333334}, {"start": 173.28, "end": 173.86, "word": " هذا", "probability": 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179.16, "word": " ال", "probability": 0.73974609375}, {"start": 179.16, "end": 179.72, "word": " high", "probability": 0.96337890625}, {"start": 179.72, "end": 180.5, "word": " oxygen", "probability": 0.95849609375}, {"start": 180.5, "end": 181.04, "word": " pressure", "probability": 0.95556640625}, {"start": 181.04, "end": 181.84, "word": " بيبدأ", "probability": 0.96875}, {"start": 181.84, "end": 182.48, "word": " يستلم", "probability": 0.9581705729166666}, {"start": 182.48, "end": 183.24, "word": " يحمل", "probability": 0.9007161458333334}, {"start": 183.24, "end": 183.84, "word": " oxygen", "probability": 0.7890625}, {"start": 183.84, "end": 185.0, "word": " تمام؟", "probability": 0.9132486979166666}], "temperature": 1.0}, {"id": 8, "seek": 19565, "start": 185.99, "end": 195.65, "text": "و في ال low oxygen pressure عند ال tissue بيبدأ يترك oxygen عشان هيك بنحكي عنه ناقل career، delivery لال oxygen", "tokens": [2407, 8978, 2423, 2295, 9169, 3321, 43242, 2423, 12404, 4724, 1829, 44510, 10721, 7251, 2655, 31747, 9169, 6225, 8592, 7649, 39896, 4117, 44945, 5016, 4117, 1829, 18871, 3224, 8717, 995, 4587, 1211, 3988, 12399, 8982, 5296, 6027, 9169], "avg_logprob": -0.20312499465086523, "compression_ratio": 1.1953125, "no_speech_prob": 1.1920928955078125e-07, "words": [{"start": 185.99, "end": 186.29, "word": "و", "probability": 0.74755859375}, {"start": 186.29, "end": 186.71, "word": " في", "probability": 0.87646484375}, {"start": 186.71, "end": 187.03, "word": " ال", "probability": 0.98779296875}, {"start": 187.03, "end": 187.27, "word": " low", "probability": 0.900390625}, {"start": 187.27, "end": 187.73, "word": " oxygen", "probability": 0.449462890625}, {"start": 187.73, "end": 188.21, "word": " pressure", "probability": 0.96533203125}, {"start": 188.21, "end": 188.51, "word": " عند", "probability": 0.982421875}, {"start": 188.51, "end": 188.69, "word": " ال", "probability": 0.86328125}, {"start": 188.69, "end": 188.95, "word": " tissue", "probability": 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0.85302734375}], "temperature": 1.0}, {"id": 9, "seek": 22545, "start": 196.91, "end": 225.45, "text": "Homoglobin also returns CO2 from the tissue to the lung فالمقابل هذا ال homoglobin بي .. مقابل انه بيعطي ال tissue اكسوجين هو يحمل ال CO2 من ال tissue و بيرجعها لل lung علشان تخرج في عملية الزفير هذا ال homoglobin molecule يتكون من جزئين شق بروتيني بنسميه globin", "tokens": [39, 298, 45841, 13496, 611, 11247, 3002, 17, 490, 264, 12404, 281, 264, 16730, 6156, 45340, 4587, 16758, 1211, 23758, 2423, 3655, 45841, 13496, 4724, 1829, 4386, 3714, 4587, 16758, 1211, 16472, 3224, 4724, 40228, 9566, 1829, 2423, 12404, 1975, 4117, 3794, 29245, 9957, 31439, 7251, 35571, 1211, 2423, 3002, 17, 9154, 2423, 12404, 4032, 4724, 13546, 7435, 3615, 11296, 24976, 16730, 11203, 8592, 7649, 6055, 34740, 7435, 8978, 6225, 42213, 10632, 6024, 110, 5172, 13546, 23758, 2423, 3655, 45841, 13496, 15582, 7251, 2655, 30544, 9154, 10874, 11622, 19986, 9957, 13412, 4587, 4724, 32887, 2655, 9957, 1829, 44945, 38251, 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"probability": 0.724609375}, {"start": 210.37, "end": 211.05, "word": " يحمل", "probability": 0.9930013020833334}, {"start": 211.05, "end": 211.29, "word": " ال", "probability": 0.98193359375}, {"start": 211.29, "end": 212.07, "word": " CO2", "probability": 0.898681640625}, {"start": 212.07, "end": 212.63, "word": " من", "probability": 0.9970703125}, {"start": 212.63, "end": 212.87, "word": " ال", "probability": 0.984375}, {"start": 212.87, "end": 213.15, "word": " tissue", "probability": 0.98681640625}, {"start": 213.15, "end": 213.61, "word": " و", "probability": 0.95751953125}, {"start": 213.61, "end": 214.41, "word": " بيرجعها", "probability": 0.94365234375}, {"start": 214.41, "end": 214.65, "word": " لل", "probability": 0.82421875}, {"start": 214.65, "end": 214.97, "word": " lung", "probability": 0.5830078125}, {"start": 214.97, "end": 215.61, "word": " علشان", "probability": 0.9510091145833334}, {"start": 215.61, "end": 216.19, "word": " تخرج", "probability": 0.9334309895833334}, 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الرأس", "probability": 0.687744140625}, {"start": 427.79, "end": 428.57, "word": " molecule", "probability": 0.44384765625}, {"start": 428.57, "end": 429.33, "word": " وين؟", "probability": 0.8191731770833334}, {"start": 429.33, "end": 429.51, "word": " في", "probability": 0.92041015625}, {"start": 429.51, "end": 429.67, "word": " ال", "probability": 0.9716796875}, {"start": 429.67, "end": 430.17, "word": " center", "probability": 0.81396484375}, {"start": 430.17, "end": 431.17, "word": " تمام؟", "probability": 0.96435546875}, {"start": 431.17, "end": 431.29, "word": " في", "probability": 0.97314453125}, {"start": 431.29, "end": 431.39, "word": " ال", "probability": 0.98876953125}, {"start": 431.39, "end": 431.71, "word": " center", "probability": 0.9521484375}], "temperature": 1.0}, {"id": 19, "seek": 45593, "start": 432.57, "end": 455.93, "text": "without the iron بدون ال iron هذا نخب ال iron شوية without the iron هاي الحلقة بنسميها porphyrin ring مع ال iron بتصير heme without iron porphyrin with iron heme تمام؟ فلازم انه نعرف هاي الأساسيات عن تركيب ال homoglobin", "tokens": [11820, 346, 264, 6497, 47525, 11536, 2423, 6497, 23758, 8717, 9778, 3555, 2423, 6497, 13412, 2407, 10632, 1553, 264, 6497, 8032, 47302, 21542, 1211, 28671, 44945, 38251, 1829, 11296, 1515, 950, 6016, 259, 4875, 20449, 2423, 6497, 39894, 9381, 13546, 415, 1398, 1553, 6497, 1515, 950, 6016, 259, 365, 6497, 415, 1398, 46811, 10943, 22807, 6156, 1211, 31377, 2304, 16472, 3224, 8717, 3615, 28480, 8032, 47302, 16247, 3794, 32277, 1829, 9307, 18871, 6055, 31747, 1829, 3555, 2423, 3655, 45841, 13496], "avg_logprob": -0.1783371854711462, "compression_ratio": 1.6129032258064515, "no_speech_prob": 4.827976226806641e-06, "words": [{"start": 432.57, "end": 433.41, "word": "without", "probability": 0.801025390625}, {"start": 433.41, "end": 433.61, "word": " the", "probability": 0.900390625}, {"start": 433.61, "end": 434.19, "word": " iron", "probability": 0.9287109375}, {"start": 434.19, "end": 434.67, "word": " بدون", "probability": 0.617919921875}, {"start": 434.67, "end": 434.81, "word": " ال", "probability": 0.9208984375}, {"start": 434.81, "end": 435.15, "word": " iron", "probability": 0.662109375}, {"start": 435.15, "end": 435.55, "word": " هذا", "probability": 0.68408203125}, {"start": 435.55, "end": 436.11, "word": " نخب", "probability": 0.7368977864583334}, {"start": 436.11, "end": 436.23, "word": " ال", "probability": 0.77392578125}, {"start": 436.23, "end": 436.59, "word": " iron", "probability": 0.96826171875}, {"start": 436.59, "end": 437.05, "word": " شوية", "probability": 0.8875325520833334}, {"start": 437.05, "end": 437.41, "word": " without", "probability": 0.76025390625}, {"start": 437.41, "end": 437.71, "word": " the", "probability": 0.8671875}, {"start": 437.71, "end": 438.07, "word": " iron", "probability": 0.91552734375}, {"start": 438.07, "end": 438.25, "word": " هاي", "probability": 0.580810546875}, {"start": 438.25, "end": 438.67, "word": " الحلقة", "probability": 0.9903971354166666}, {"start": 438.67, "end": 439.49, "word": " بنسميها", "probability": 0.9576416015625}, {"start": 439.49, "end": 440.71, "word": " porphyrin", "probability": 0.84033203125}, {"start": 440.71, "end": 441.15, "word": " ring", "probability": 0.97607421875}, {"start": 441.15, "end": 441.89, "word": " مع", "probability": 0.96435546875}, {"start": 441.89, "end": 442.09, "word": " ال", "probability": 0.97265625}, {"start": 442.09, "end": 442.57, "word": " iron", "probability": 0.9599609375}, {"start": 442.57, "end": 443.13, "word": " بتصير", "probability": 0.9513346354166666}, {"start": 443.13, "end": 443.65, "word": " heme", "probability": 0.6669921875}, {"start": 443.65, "end": 444.65, "word": " without", "probability": 0.6474609375}, {"start": 444.65, "end": 445.53, "word": " iron", "probability": 0.8955078125}, {"start": 445.53, "end": 446.79, "word": " porphyrin", "probability": 0.937255859375}, {"start": 446.79, "end": 447.37, "word": " with", 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0.9140625}], "temperature": 1.0}, {"id": 20, "seek": 48303, "start": 457.07, "end": 483.03, "text": "normal ranges المدى الطبيعي أو المستوى الطبيعي لأ ال homoglobin طبعا احنا بنقيس ال homoglobin في جسمنا سواء كان انا بحكيلك فحص homoglobin او فحص total homoglobin ف it's the same نفس المعنى طب ايش يعني total homoglobin؟ طب ليش احنا اضفنا كلمة total قبل homoglobin؟", "tokens": [23157, 22526, 9673, 3215, 7578, 41950, 21292, 3615, 1829, 34051, 9673, 14851, 2407, 7578, 41950, 21292, 3615, 1829, 5296, 10721, 2423, 3655, 45841, 13496, 23032, 3555, 3615, 995, 1975, 5016, 8315, 44945, 4587, 1829, 3794, 2423, 3655, 45841, 13496, 8978, 10874, 38251, 8315, 8608, 2407, 16606, 25961, 1975, 8315, 4724, 5016, 4117, 1829, 23275, 6156, 5016, 9381, 3655, 45841, 13496, 1975, 2407, 6156, 5016, 9381, 3217, 3655, 45841, 13496, 6156, 309, 311, 264, 912, 8717, 36178, 9673, 3615, 1863, 7578, 23032, 3555, 1975, 1829, 8592, 37495, 22653, 3217, 3655, 45841, 13496, 22807, 23032, 3555, 32239, 8592, 1975, 5016, 8315, 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homoglobin", "probability": 0.6973470052083334}, {"start": 462.57, "end": 463.81, "word": " طبعا", "probability": 0.9833984375}, {"start": 463.81, "end": 464.53, "word": " احنا", "probability": 0.8836263020833334}, {"start": 464.53, "end": 465.63, "word": " بنقيس", "probability": 0.8118896484375}, {"start": 465.63, "end": 466.29, "word": " ال", "probability": 0.9892578125}, {"start": 466.29, "end": 467.17, "word": " homoglobin", "probability": 0.9474283854166666}, {"start": 467.17, "end": 467.95, "word": " في", "probability": 0.88232421875}, {"start": 467.95, "end": 468.55, "word": " جسمنا", "probability": 0.9781901041666666}, {"start": 468.55, "end": 468.77, "word": " سواء", "probability": 0.9401041666666666}, {"start": 468.77, "end": 469.07, "word": " كان", "probability": 0.97705078125}, {"start": 469.07, "end": 469.31, "word": " انا", "probability": 0.822509765625}, {"start": 469.31, "end": 469.83, "word": " بحكيلك", "probability": 0.93876953125}, {"start": 469.83, "end": 470.25, "word": " فحص", "probability": 0.9632161458333334}, {"start": 470.25, "end": 470.93, "word": " homoglobin", "probability": 0.9588216145833334}, {"start": 470.93, "end": 471.41, "word": " او", "probability": 0.834228515625}, {"start": 471.41, "end": 471.83, "word": " فحص", "probability": 0.9697265625}, {"start": 471.83, "end": 472.75, "word": " total", "probability": 0.87353515625}, {"start": 472.75, "end": 473.81, "word": " homoglobin", "probability": 0.96044921875}, {"start": 473.81, "end": 474.47, "word": " ف", "probability": 0.95947265625}, {"start": 474.47, "end": 474.79, "word": " it's", "probability": 0.8837890625}, {"start": 474.79, "end": 475.01, "word": " the", "probability": 0.947265625}, {"start": 475.01, "end": 475.35, "word": " same", "probability": 0.91259765625}, {"start": 475.35, "end": 475.77, "word": " نفس", "probability": 0.885498046875}, {"start": 475.77, "end": 476.59, "word": " المعنى", "probability": 0.9884033203125}, {"start": 476.59, "end": 476.93, "word": " طب", "probability": 0.869873046875}, {"start": 476.93, "end": 477.15, "word": " ايش", "probability": 0.77783203125}, {"start": 477.15, "end": 477.47, "word": " يعني", "probability": 0.93603515625}, {"start": 477.47, "end": 477.81, "word": " total", "probability": 0.9140625}, {"start": 477.81, "end": 478.55, "word": " homoglobin؟", "probability": 0.9033203125}, {"start": 478.55, "end": 478.67, "word": " طب", "probability": 0.9423828125}, {"start": 478.67, "end": 478.85, "word": " ليش", "probability": 0.91748046875}, {"start": 478.85, "end": 479.11, "word": " احنا", "probability": 0.9767252604166666}, {"start": 479.11, "end": 480.01, "word": " اضفنا", "probability": 0.86474609375}, {"start": 480.01, "end": 480.43, "word": " كلمة", "probability": 0.9861653645833334}, {"start": 480.43, "end": 480.95, "word": " total", "probability": 0.8974609375}, {"start": 480.95, "end": 481.71, "word": " قبل", "probability": 0.9912109375}, {"start": 481.71, "end": 483.03, "word": " homoglobin؟", "probability": 0.9342041015625}], "temperature": 1.0}, {"id": 21, "seek": 49688, "start": 483.18, "end": 496.88, "text": "لأن يا بنات أنواع ال homoglobin في خلايا ال RBCs مش واحدة، تمام؟ شوفوا معايا، تتذكروا احنا ذكرنا معلومة سابقا حكينا انه", "tokens": [1211, 33456, 35186, 4724, 8315, 2655, 14739, 14407, 3615, 2423, 3655, 45841, 13496, 8978, 16490, 15040, 25528, 2423, 497, 7869, 82, 37893, 36764, 24401, 3660, 12399, 46811, 10943, 22807, 13412, 38688, 14407, 20449, 995, 25528, 12399, 6055, 2655, 8848, 37983, 14407, 1975, 5016, 8315, 29910, 37983, 8315, 20449, 1211, 20498, 3660, 8608, 16758, 4587, 995, 11331, 4117, 1829, 8315, 16472, 3224], "avg_logprob": -0.11813256168557752, "compression_ratio": 1.380952380952381, "no_speech_prob": 1.1920928955078125e-06, "words": [{"start": 483.18, "end": 483.7, "word": "لأن", "probability": 0.827392578125}, {"start": 483.7, "end": 483.9, "word": " يا", "probability": 0.54150390625}, {"start": 483.9, "end": 484.52, "word": " بنات", "probability": 0.9524739583333334}, {"start": 484.52, "end": 485.66, "word": " أنواع", "probability": 0.9114583333333334}, {"start": 485.66, "end": 485.9, "word": " ال", "probability": 0.9892578125}, {"start": 485.9, "end": 486.98, "word": " homoglobin", "probability": 0.7589518229166666}, {"start": 486.98, "end": 487.48, "word": " في", "probability": 0.94970703125}, {"start": 487.48, "end": 488.04, "word": " خلايا", "probability": 0.9466145833333334}, {"start": 488.04, "end": 488.2, "word": " ال", "probability": 0.978515625}, {"start": 488.2, "end": 489.08, "word": " RBCs", "probability": 0.7747395833333334}, {"start": 489.08, "end": 489.56, "word": " مش", "probability": 0.9658203125}, {"start": 489.56, "end": 490.36, "word": " واحدة،", "probability": 0.8572998046875}, {"start": 490.36, "end": 491.26, "word": " تمام؟", "probability": 0.9306640625}, {"start": 491.26, "end": 491.58, "word": " شوفوا", "probability": 0.9812825520833334}, {"start": 491.58, "end": 492.4, "word": " معايا،", "probability": 0.9119873046875}, {"start": 492.4, "end": 493.02, "word": " تتذكروا", "probability": 0.9130859375}, {"start": 493.02, "end": 493.3, "word": " احنا", "probability": 0.9150390625}, {"start": 493.3, "end": 494.16, "word": " ذكرنا", "probability": 0.96923828125}, {"start": 494.16, "end": 494.86, "word": " معلومة", "probability": 0.9893798828125}, {"start": 494.86, "end": 495.48, "word": " سابقا", "probability": 0.9451904296875}, {"start": 495.48, "end": 496.12, "word": " حكينا", "probability": 0.984619140625}, {"start": 496.12, "end": 496.88, "word": " انه", "probability": 0.707275390625}], "temperature": 1.0}, {"id": 22, "seek": 52409, "start": 498.33, "end": 524.09, "text": "الأطفال الحديثي الولادة بيكون عندهم نوع ال homoglobin اللي له high affinity مع ال oxygen عشان هيك مش بسهولة بيترك ال oxygen عند ال tissue عشان هيك ربنا عوضهم بنسبة RBCs ونسبة homoglobin عالية بالاضافة لإنه أيضا عندهم growth pairs طيب هدول حكيتلكم عندهم النوع ال homoglobin F homoglobin F اللي هو ال fetal homoglobin", 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0.97119140625}, {"start": 592.6, "end": 592.9, "word": " affinity", "probability": 0.97900390625}, {"start": 592.9, "end": 593.3, "word": " تبعته", "probability": 0.965087890625}, {"start": 593.3, "end": 593.92, "word": " معتدلة", "probability": 0.930908203125}, {"start": 593.92, "end": 594.7, "word": " بتكون", "probability": 0.880615234375}, {"start": 594.7, "end": 595.26, "word": " عالية", "probability": 0.9910481770833334}, {"start": 595.26, "end": 595.8, "word": " و", "probability": 0.44091796875}, {"start": 595.8, "end": 596.1, "word": " هذا", "probability": 0.88330078125}, {"start": 596.1, "end": 596.22, "word": " ال", "probability": 0.923828125}, {"start": 596.22, "end": 596.8, "word": " homoglobin", "probability": 0.8299153645833334}, {"start": 596.8, "end": 597.42, "word": " بسميه", "probability": 0.9814453125}, {"start": 597.42, "end": 598.04, "word": " homoglobin", "probability": 0.931640625}, {"start": 598.04, "end": 598.44, "word": " A", "probability": 0.72119140625}, 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نسب ضئيلة من homoglobin F and homoglobin A2 عشان هيك لما أجي أحكي أنا بعمل فحص لأ ال total homoglobin أنا بكون عم بذكر ال homoglobin A", "tokens": [41185, 18871, 16254, 14739, 14407, 3615, 5551, 34740, 7578, 23032, 3555, 3615, 995, 37495, 22653, 41850, 5296, 15042, 4724, 5016, 4117, 1829, 14739, 3224, 41850, 8978, 16490, 15040, 1829, 7649, 995, 8978, 13420, 4, 34051, 5551, 4117, 49115, 9154, 2423, 3655, 45841, 13496, 316, 6156, 21292, 30544, 8978, 18871, 16254, 8717, 35457, 48812, 19986, 26895, 3660, 5296, 9307, 4117, 18513, 6055, 8848, 37983, 9154, 2423, 3655, 45841, 13496, 479, 4032, 2423, 3655, 45841, 13496, 316, 17, 46811, 10943, 22807, 8717, 35457, 48812, 19986, 26895, 3660, 9154, 3655, 45841, 13496, 479, 293, 3655, 45841, 13496, 316, 17, 6225, 8592, 7649, 39896, 4117, 5296, 15042, 5551, 7435, 1829, 5551, 5016, 4117, 1829, 41850, 4724, 25957, 1211, 6156, 5016, 9381, 5296, 10721, 2423, 3217, 3655, 45841, 13496, 41850, 4724, 30544, 6225, 2304, 4724, 8848, 37983, 2423, 3655, 45841, 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"probability": 0.969970703125}, {"start": 624.37, "end": 624.59, "word": " لما", "probability": 0.93115234375}, {"start": 624.59, "end": 624.89, "word": " أجي", "probability": 0.8352864583333334}, {"start": 624.89, "end": 625.23, "word": " أحكي", "probability": 0.97021484375}, {"start": 625.23, "end": 625.39, "word": " أنا", "probability": 0.6123046875}, {"start": 625.39, "end": 626.19, "word": " بعمل", "probability": 0.90771484375}, {"start": 626.19, "end": 626.61, "word": " فحص", "probability": 0.9728190104166666}, {"start": 626.61, "end": 626.91, "word": " لأ", "probability": 0.87255859375}, {"start": 626.91, "end": 627.13, "word": " ال", "probability": 0.92578125}, {"start": 627.13, "end": 627.77, "word": " total", "probability": 0.8876953125}, {"start": 627.77, "end": 629.05, "word": " homoglobin", "probability": 0.9137369791666666}, {"start": 629.05, "end": 629.51, "word": " أنا", "probability": 0.7099609375}, {"start": 629.51, "end": 629.95, "word": " بكون", "probability": 0.98583984375}, {"start": 629.95, "end": 630.23, "word": " عم", "probability": 0.831787109375}, {"start": 630.23, "end": 630.65, "word": " بذكر", "probability": 0.8693033854166666}, {"start": 630.65, "end": 630.99, "word": " ال", "probability": 0.9423828125}, {"start": 630.99, "end": 631.63, "word": " homoglobin", "probability": 0.9571940104166666}, {"start": 631.63, "end": 631.95, "word": " A", "probability": 0.98681640625}], "temperature": 1.0}, {"id": 28, "seek": 65908, "start": 632.66, "end": 659.08, "text": "اللي هو ال adult homoglobin ال homoglobin A2 و ال homoglobin F طب بقاشى بيختلفوا هدول الأنواع و ليش احنا هيك بنسميهم مش كلهم homoglobin و السلام لإنهم بيختلفوا بتركيبهم بيختلفوا بالoglobin part تبعهم مش طبعا ال alpha homoglobin او sorry ال homoglobin A اللي هو ال adult homoglobin هذا هو اللي بيتكون من تنتين alpha تنتين beta chains", "tokens": [6027, 20292, 31439, 2423, 5075, 3655, 45841, 13496, 2423, 3655, 45841, 13496, 316, 17, 4032, 2423, 3655, 45841, 13496, 479, 23032, 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"word": " بيختلفوا", "probability": 0.985546875}, {"start": 638.52, "end": 638.82, "word": " هدول", "probability": 0.9552408854166666}, {"start": 638.82, "end": 639.56, "word": " الأنواع", "probability": 0.9229736328125}, {"start": 639.56, "end": 639.84, "word": " و", "probability": 0.466064453125}, {"start": 639.84, "end": 640.02, "word": " ليش", "probability": 0.903564453125}, {"start": 640.02, "end": 640.26, "word": " احنا", "probability": 0.9064127604166666}, {"start": 640.26, "end": 640.48, "word": " هيك", "probability": 0.857666015625}, {"start": 640.48, "end": 641.06, "word": " بنسميهم", "probability": 0.859375}, {"start": 641.06, "end": 641.24, "word": " مش", "probability": 0.67578125}, {"start": 641.24, "end": 641.82, "word": " كلهم", "probability": 0.75146484375}, {"start": 641.82, "end": 642.3, "word": " homoglobin", "probability": 0.79345703125}, {"start": 642.3, "end": 642.42, "word": " و", "probability": 0.5615234375}, {"start": 642.42, "end": 642.92, "word": " السلام", "probability": 0.940673828125}, {"start": 642.92, "end": 643.86, "word": " لإنهم", "probability": 0.8720703125}, {"start": 643.86, "end": 644.54, "word": " بيختلفوا", "probability": 0.98544921875}, {"start": 644.54, "end": 645.4, "word": " بتركيبهم", "probability": 0.95458984375}, {"start": 645.4, "end": 646.46, "word": " بيختلفوا", "probability": 0.93076171875}, {"start": 646.46, "end": 647.26, "word": " بالoglobin", "probability": 0.6165771484375}, {"start": 647.26, "end": 647.74, "word": " part", "probability": 0.91845703125}, {"start": 647.74, "end": 648.48, "word": " تبعهم", "probability": 0.91259765625}, {"start": 648.48, "end": 649.16, "word": " مش", "probability": 0.86328125}, {"start": 649.16, "end": 649.76, "word": " طبعا", "probability": 0.93505859375}, {"start": 649.76, "end": 649.88, "word": " ال", "probability": 0.958984375}, {"start": 649.88, "end": 650.28, "word": " alpha", "probability": 0.767578125}, {"start": 650.28, "end": 651.2, "word": " homoglobin", 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0.98388671875}, {"start": 682.38, "end": 683.1, "word": " مكوّن", "probability": 0.93935546875}, {"start": 683.1, "end": 683.26, "word": " من", "probability": 0.99560546875}, {"start": 683.26, "end": 683.74, "word": " سلاسل", "probability": 0.9036865234375}, {"start": 683.74, "end": 684.3, "word": " أخرى", "probability": 0.9720052083333334}, {"start": 684.3, "end": 684.72, "word": " راح", "probability": 0.7734375}, {"start": 684.72, "end": 685.36, "word": " تتعرفوا", "probability": 0.97900390625}, {"start": 685.36, "end": 685.7, "word": " عليها", "probability": 0.992919921875}, {"start": 685.7, "end": 686.34, "word": " بمساقل", "probability": 0.8448486328125}, {"start": 686.34, "end": 687.04, "word": " hematology", "probability": 0.7482096354166666}, {"start": 687.04, "end": 687.82, "word": " لما", "probability": 0.7340087890625}, {"start": 687.82, "end": 688.14, "word": " تصيروا", "probability": 0.9227294921875}, {"start": 688.14, "end": 688.34, "word": " سنة", "probability": 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منهم الـ adult صح هو الأكثر شيوعا هو المكون من تنتين Alpha تنتين Beta عندنا ك adult تمام؟ ولكن في عندنا لسه ضايق جزء من اللي هو ال homoglobin اللي كان عندنا في ولادها homoglobin F وعندنا جزء ضئيل أيضا من نوع آخر اسمه homoglobin A2 تمام؟ عشان هيك لما أحكيلك نقيس ال homoglobin أو ال total homoglobin it's the same نفس المعنى", "tokens": [15718, 4, 9154, 16095, 2423, 39184, 5075, 20328, 5016, 31439, 16247, 4117, 49115, 13412, 1829, 45367, 995, 31439, 9673, 30544, 9154, 6055, 29399, 9957, 20588, 6055, 29399, 9957, 33286, 43242, 8315, 9122, 5075, 46811, 10943, 22807, 35525, 19452, 8978, 43242, 8315, 5296, 3794, 3224, 48812, 47302, 4587, 10874, 11622, 38207, 9154, 13672, 1829, 31439, 2423, 3655, 45841, 13496, 13672, 1829, 25961, 43242, 8315, 8978, 49429, 3215, 11296, 3655, 45841, 13496, 479, 4032, 3615, 41260, 8315, 10874, 11622, 38207, 48812, 19986, 26895, 36632, 11242, 995, 9154, 8717, 45367, 19753, 34740, 24525, 2304, 3224, 3655, 45841, 13496, 316, 17, 46811, 10943, 22807, 6225, 8592, 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{"start": 878.36, "end": 878.82, "word": " homoglobin", "probability": 0.9601236979166666}, {"start": 878.82, "end": 879.0, "word": " أو", "probability": 0.76220703125}, {"start": 879.0, "end": 879.12, "word": " ال", "probability": 0.98193359375}, {"start": 879.12, "end": 879.34, "word": " adult", "probability": 0.87841796875}, {"start": 879.34, "end": 879.78, "word": " range", "probability": 0.93115234375}, {"start": 879.78, "end": 879.94, "word": " of", "probability": 0.97412109375}, {"start": 879.94, "end": 880.58, "word": " homoglobin", "probability": 0.9518229166666666}, {"start": 880.58, "end": 881.08, "word": " يعني", "probability": 0.740478515625}, {"start": 881.08, "end": 881.28, "word": " هو", "probability": 0.984375}, {"start": 881.28, "end": 881.52, "word": " ال", "probability": 0.90869140625}, {"start": 881.52, "end": 881.76, "word": " ..", "probability": 0.93359375}], "temperature": 1.0}, {"id": 39, "seek": 90384, "start": 881.8, "end": 903.84, "text": "ال range المتعارف عليه انه يعني ال adult men من 14 ل 18 gram per deciliter و ال woman من 12 ل 16 gram per deciliter عندنا ال woman after middle age بعد منتصف عمرها مع ال blood loss or dehumidification و الحمل و الولادة و ال physiological need تبعتها", "tokens": [6027, 3613, 9673, 2655, 3615, 9640, 5172, 47356, 16472, 3224, 37495, 22653, 2423, 5075, 1706, 9154, 3499, 5296, 2443, 21353, 680, 979, 388, 1681, 4032, 2423, 3059, 9154, 2272, 5296, 3165, 21353, 680, 979, 388, 1681, 43242, 8315, 2423, 3059, 934, 2808, 3205, 39182, 9154, 2655, 9381, 5172, 6225, 29973, 11296, 20449, 2423, 3390, 4470, 420, 368, 14645, 327, 3774, 4032, 21542, 42213, 4032, 2423, 12610, 18513, 3660, 4032, 2423, 41234, 643, 6055, 3555, 34268, 11296], "avg_logprob": -0.20819805194805194, "compression_ratio": 1.5545023696682465, "no_speech_prob": 9.5367431640625e-07, "words": [{"start": 881.8, "end": 882.18, "word": "ال", "probability": 0.84814453125}, {"start": 882.18, "end": 883.08, "word": " range", "probability": 0.52001953125}, {"start": 883.08, "end": 883.76, "word": " المتعارف", "probability": 0.9677734375}, {"start": 883.76, "end": 884.14, "word": " عليه", "probability": 0.460205078125}, {"start": 884.14, "end": 884.78, "word": " انه", "probability": 0.5577392578125}, {"start": 884.78, "end": 885.12, "word": " يعني", "probability": 0.966552734375}, {"start": 885.12, "end": 885.34, "word": " ال", "probability": 0.97119140625}, {"start": 885.34, "end": 885.68, "word": " adult", "probability": 0.77392578125}, {"start": 885.68, "end": 885.96, "word": " men", "probability": 0.642578125}, {"start": 885.96, "end": 886.12, "word": " من", "probability": 0.9912109375}, {"start": 886.12, "end": 886.82, "word": " 14", "probability": 0.87255859375}, {"start": 886.82, "end": 887.34, "word": " ل", "probability": 0.67626953125}, {"start": 887.34, "end": 887.86, "word": " 18", "probability": 0.7734375}, {"start": 887.86, "end": 888.32, "word": " gram", "probability": 0.87646484375}, {"start": 888.32, "end": 888.5, "word": " per", "probability": 0.7744140625}, {"start": 888.5, "end": 889.14, "word": " deciliter", "probability": 0.8580729166666666}, {"start": 889.14, "end": 889.62, "word": " و", "probability": 0.9423828125}, {"start": 889.62, "end": 889.88, "word": " ال", "probability": 0.9306640625}, {"start": 889.88, "end": 890.14, "word": " woman", "probability": 0.60986328125}, {"start": 890.14, "end": 890.36, "word": " من", "probability": 0.99267578125}, {"start": 890.36, "end": 890.64, "word": " 12", "probability": 0.95947265625}, {"start": 890.64, "end": 890.76, "word": " ل", "probability": 0.974609375}, {"start": 890.76, "end": 891.16, "word": " 16", "probability": 0.92724609375}, {"start": 891.16, "end": 891.56, "word": " gram", "probability": 0.95947265625}, {"start": 891.56, "end": 891.74, "word": " per", "probability": 0.95751953125}, {"start": 891.74, "end": 892.36, "word": " deciliter", "probability": 0.92333984375}, {"start": 892.36, "end": 893.26, "word": " عندنا", "probability": 0.928955078125}, {"start": 893.26, "end": 893.48, "word": " ال", "probability": 0.923828125}, {"start": 893.48, "end": 893.8, "word": " woman", "probability": 0.685546875}, {"start": 893.8, "end": 894.24, "word": " after", "probability": 0.8876953125}, {"start": 894.24, "end": 894.56, "word": " middle", "probability": 0.89013671875}, {"start": 894.56, "end": 894.82, "word": " age", "probability": 0.865234375}, {"start": 894.82, "end": 895.06, "word": " بعد", "probability": 0.93994140625}, {"start": 895.06, "end": 895.54, "word": " منتصف", "probability": 0.98828125}, {"start": 895.54, "end": 896.12, "word": " عمرها", "probability": 0.9925130208333334}, {"start": 896.12, "end": 896.58, "word": " مع", "probability": 0.97216796875}, {"start": 896.58, "end": 896.74, "word": " ال", "probability": 0.796875}, {"start": 896.74, "end": 897.06, "word": " blood", "probability": 0.9697265625}, {"start": 897.06, "end": 897.44, "word": " loss", "probability": 0.8857421875}, {"start": 897.44, "end": 898.0, "word": " or", "probability": 0.2626953125}, {"start": 898.0, "end": 899.0, "word": " dehumidification", "probability": 0.496429443359375}, {"start": 899.0, "end": 899.7, "word": " و", "probability": 0.8779296875}, {"start": 899.7, "end": 901.3, "word": " الحمل", "probability": 0.71240234375}, {"start": 901.3, "end": 901.48, "word": " و", "probability": 0.97412109375}, {"start": 901.48, "end": 902.14, "word": " الولادة", "probability": 0.9854736328125}, {"start": 902.14, "end": 902.32, "word": " و", "probability": 0.9970703125}, {"start": 902.32, "end": 902.4, "word": " ال", "probability": 0.873046875}, {"start": 902.4, "end": 902.82, "word": " physiological", "probability": 0.23193359375}, {"start": 902.82, "end": 903.22, "word": " need", "probability": 0.927734375}, {"start": 903.22, "end": 903.84, "word": " تبعتها", "probability": 0.922119140625}], "temperature": 1.0}, {"id": 40, "seek": 93060, "start": 904.02, "end": 930.6, "text": "فبصير عندها نسبة ال homoglobin من 11.7 ل 13.8 أو من 11 ل 14 gram per deciliter above normal homoglobin levels is called polycythemia عندي homoglobin أعلى من الطبيعي بسمي هذه الحالة polycythemia و below normal homoglobin level homoglobin أقل من الطبيعي بسمي هذه الحالة anemia", "tokens": [5172, 3555, 9381, 13546, 43242, 11296, 8717, 35457, 3660, 2423, 3655, 45841, 13496, 9154, 2975, 13, 22, 5296, 3705, 13, 23, 34051, 9154, 2975, 5296, 3499, 21353, 680, 979, 388, 1681, 3673, 2710, 3655, 45841, 13496, 4358, 307, 1219, 6754, 1344, 392, 14058, 18871, 16254, 3655, 45841, 13496, 5551, 3615, 23942, 9154, 41950, 21292, 3615, 1829, 4724, 38251, 1829, 29538, 21542, 6027, 3660, 6754, 1344, 392, 14058, 4032, 2507, 2710, 3655, 45841, 13496, 1496, 3655, 45841, 13496, 5551, 4587, 1211, 9154, 41950, 21292, 3615, 1829, 4724, 38251, 1829, 29538, 21542, 6027, 3660, 364, 14058], "avg_logprob": -0.16217105357270492, "compression_ratio": 1.778894472361809, "no_speech_prob": 1.7881393432617188e-07, "words": [{"start": 904.02, "end": 904.8, "word": 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الاشي بيذكرنا ال hematocrite لما ذكرنا ال hematocrite أو شرحنا ال hematocrite حكينا إنه زيادة hematocrite above the normal أعلى من ال normal بسميها polycythemia و hematocrite below normal أقل من ال normal بسميه anemia", "tokens": [2407, 3224, 15730, 2423, 33599, 1829, 4724, 1829, 8848, 37983, 8315, 2423, 8636, 267, 3617, 642, 5296, 15042, 29910, 37983, 8315, 2423, 8636, 267, 3617, 642, 34051, 13412, 2288, 5016, 8315, 2423, 8636, 267, 3617, 642, 11331, 4117, 1829, 8315, 36145, 3224, 30767, 1829, 18513, 3660, 8636, 267, 3617, 642, 3673, 264, 2710, 5551, 3615, 23942, 9154, 2423, 2710, 4724, 38251, 1829, 11296, 6754, 1344, 392, 14058, 4032, 8636, 267, 3617, 642, 2507, 2710, 5551, 4587, 1211, 9154, 2423, 2710, 4724, 38251, 1829, 3224, 364, 14058], "avg_logprob": -0.15193966133841152, "compression_ratio": 1.8231707317073171, "no_speech_prob": 1.1920928955078125e-07, "words": [{"start": 930.96, "end": 931.48, "word": "وهذا", "probability": 0.7650553385416666}, {"start": 931.48, "end": 932.0, 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حكينا", "probability": 0.9775390625}, {"start": 938.76, "end": 939.24, "word": " إنه", "probability": 0.6199951171875}, {"start": 939.24, "end": 940.2, "word": " زيادة", "probability": 0.9912109375}, {"start": 940.2, "end": 940.8, "word": " hematocrite", "probability": 0.8924560546875}, {"start": 940.8, "end": 941.18, "word": " above", "probability": 0.9755859375}, {"start": 941.18, "end": 941.44, "word": " the", "probability": 0.8955078125}, {"start": 941.44, "end": 941.84, "word": " normal", "probability": 0.8818359375}, {"start": 941.84, "end": 942.3, "word": " أعلى", "probability": 0.8777669270833334}, {"start": 942.3, "end": 942.46, "word": " من", "probability": 0.99658203125}, {"start": 942.46, "end": 942.6, "word": " ال", "probability": 0.8056640625}, {"start": 942.6, "end": 942.9, "word": " normal", "probability": 0.80908203125}, {"start": 942.9, "end": 943.46, "word": " بسميها", "probability": 0.897705078125}, {"start": 943.46, "end": 944.36, "word": " polycythemia", 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organ disorder تمام؟ وبينتج عنها زيادة في تصنيع ال RBCs يعني زيادة في ال homoglobin level", "tokens": [3224, 1211, 10721, 11933, 1829, 8592, 37495, 22653, 6754, 1344, 392, 14058, 22807, 39896, 11331, 6027, 3660, 3714, 43042, 10632, 11933, 15042, 8717, 31371, 7435, 3660, 5592, 34051, 1798, 13399, 46811, 10943, 22807, 4032, 21292, 29399, 7435, 18871, 11296, 30767, 1829, 18513, 3660, 8978, 6055, 9381, 22653, 3615, 2423, 497, 7869, 82, 37495, 22653, 30767, 1829, 18513, 3660, 8978, 2423, 3655, 45841, 13496, 1496], "avg_logprob": -0.09188701923076924, "compression_ratio": 1.3099415204678362, "no_speech_prob": 5.960464477539063e-08, "words": [{"start": 949.81, "end": 950.19, "word": "هلأ", "probability": 0.8154296875}, {"start": 950.19, "end": 950.41, "word": " إيش", "probability": 0.8806966145833334}, {"start": 950.41, "end": 950.67, "word": " يعني", "probability": 0.909423828125}, {"start": 950.67, "end": 951.61, "word": " polycythemia؟", "probability": 0.86044921875}, {"start": 951.61, 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potassium", "probability": 0.96142578125}, {"start": 1629.74, "end": 1630.48, "word": " cyanide", "probability": 0.984375}], "temperature": 1.0}, {"id": 75, "seek": 165942, "start": 1632.22, "end": 1659.42, "text": "طبعا اللي بيصير كالتالي انه هذا المحلول بيكسر ال RBC's بيطلع ال homoglobin منها ال homoglobin هذا بيتأكسد بيصيرله oxidized بفعل مين؟ بفعل الشق الأول من ال reagent .. من مكونات ال reagent اللي هو ال potassium ferricyanide طب لما بيتأكسد هذا ال homoglobin اش بيعطيني؟ بيعطيني met homoglobin حاجة اسمها met homoglobin طبعا؟", "tokens": [9566, 3555, 3615, 995, 13672, 1829, 4724, 1829, 9381, 13546, 9122, 6027, 2655, 6027, 1829, 16472, 3224, 23758, 9673, 5016, 1211, 12610, 4724, 1829, 4117, 3794, 2288, 2423, 497, 7869, 311, 4724, 1829, 9566, 1211, 3615, 2423, 3655, 45841, 13496, 9154, 11296, 2423, 3655, 45841, 13496, 23758, 4724, 36081, 10721, 4117, 3794, 3215, 4724, 1829, 9381, 13546, 43761, 19924, 1602, 4724, 5172, 30241, 3714, 9957, 22807, 4724, 5172, 30241, 25124, 4587, 16247, 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{"start": 1634.54, "end": 1634.72, "word": " هذا", "probability": 0.83349609375}, {"start": 1634.72, "end": 1635.16, "word": " المحلول", "probability": 0.9945068359375}, {"start": 1635.16, "end": 1635.66, "word": " بيكسر", "probability": 0.9736328125}, {"start": 1635.66, "end": 1635.78, "word": " ال", "probability": 0.986328125}, {"start": 1635.78, "end": 1636.3, "word": " RBC's", "probability": 0.6289876302083334}, {"start": 1636.3, "end": 1636.68, "word": " بيطلع", "probability": 0.8826171875}, {"start": 1636.68, "end": 1636.78, "word": " ال", "probability": 0.99169921875}, {"start": 1636.78, "end": 1637.34, "word": " homoglobin", "probability": 0.7609049479166666}, {"start": 1637.34, "end": 1638.04, "word": " منها", "probability": 0.990234375}, {"start": 1638.04, "end": 1638.56, "word": " ال", "probability": 0.92822265625}, {"start": 1638.56, "end": 1639.18, "word": " homoglobin", "probability": 0.9508463541666666}, {"start": 1639.18, "end": 1639.64, "word": " هذا", "probability": 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..", "probability": 0.5029296875}, {"start": 1646.66, "end": 1646.72, "word": " من", "probability": 0.8642578125}, {"start": 1646.72, "end": 1647.24, "word": " مكونات", "probability": 0.84599609375}, {"start": 1647.24, "end": 1647.34, "word": " ال", "probability": 0.98779296875}, {"start": 1647.34, "end": 1647.64, "word": " reagent", "probability": 0.9853515625}, {"start": 1647.64, "end": 1647.78, "word": " اللي", "probability": 0.950439453125}, {"start": 1647.78, "end": 1647.88, "word": " هو", "probability": 0.994140625}, {"start": 1647.88, "end": 1647.98, "word": " ال", "probability": 0.89892578125}, {"start": 1647.98, "end": 1648.6, "word": " potassium", "probability": 0.9501953125}, {"start": 1648.6, "end": 1649.74, "word": " ferricyanide", "probability": 0.8648681640625}, {"start": 1649.74, "end": 1650.4, "word": " طب", "probability": 0.93212890625}, {"start": 1650.4, "end": 1650.78, "word": " لما", "probability": 0.802001953125}, {"start": 1650.78, "end": 1651.44, "word": " بيتأكسد", "probability": 0.9737141927083334}, {"start": 1651.44, "end": 1651.62, "word": " هذا", "probability": 0.931640625}, {"start": 1651.62, "end": 1651.74, "word": " ال", "probability": 0.9814453125}, {"start": 1651.74, "end": 1652.22, "word": " homoglobin", "probability": 0.9599609375}, {"start": 1652.22, "end": 1652.44, "word": " اش", "probability": 0.5281982421875}, {"start": 1652.44, "end": 1653.68, "word": " بيعطيني؟", "probability": 0.784912109375}, {"start": 1653.68, "end": 1654.84, "word": " بيعطيني", "probability": 0.95810546875}, {"start": 1654.84, "end": 1655.7, "word": " met", "probability": 0.75244140625}, {"start": 1655.7, "end": 1656.46, "word": " homoglobin", "probability": 0.8972981770833334}, {"start": 1656.46, "end": 1656.78, "word": " حاجة", "probability": 0.9396158854166666}, {"start": 1656.78, "end": 1657.2, "word": " اسمها", "probability": 0.9464518229166666}, {"start": 1657.2, "end": 1657.64, "word": " met", "probability": 0.9404296875}, {"start": 1657.64, 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"end": 1684.84, "word": " 540", "probability": 0.874267578125}, {"start": 1684.84, "end": 1685.9, "word": " nanometer", "probability": 0.692626953125}, {"start": 1685.9, "end": 1686.9, "word": " تمام؟", "probability": 0.93701171875}, {"start": 1686.9, "end": 1687.04, "word": " على", "probability": 0.841796875}, {"start": 1687.04, "end": 1687.78, "word": " 540", "probability": 0.92578125}, {"start": 1687.78, "end": 1688.38, "word": " nanometer", "probability": 0.948486328125}], "temperature": 1.0}, {"id": 77, "seek": 171939, "start": 1690.49, "end": 1719.39, "text": "إذا الـ Homoglobin اللي موجود في ال blood بيكون في iron بالferrous state اللي هو إيش يعني؟ بحالة الferrous اللي هي plus 2 يتأكسد بفعل ال potassium ferricyanide إلى met homoglobin وهذا ال .. ال homoglobin المؤكسد بما يختلف عن ال homoglobin الطبيعي بإنه ال iron تحول من ferrous إلى ferric من plus 2", "tokens": [28814, 15730, 2423, 39184, 20903, 45841, 13496, 13672, 1829, 3714, 29245, 23328, 8978, 2423, 3390, 4724, 1829, 30544, 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met-homoglobin بفعل الـ potassium cyanide يتحول إلى cyanomet-homoglobin colored substance يتم قراءتها على 540 نانومتر وبنقيص طبعاً كمية الـ cyanomet-homoglobin اللي هتعكس لكمية الـ homoglobin في العينة على طول الموج 540 نانومتر باستخدام جهاز الـ spectrophotometer", "tokens": [3224, 15730, 2423, 39184, 1131, 12, 71, 298, 45841, 13496, 4724, 5172, 30241, 2423, 39184, 29547, 47463, 482, 7251, 2655, 5016, 12610, 30731, 47463, 649, 12, 71, 298, 45841, 13496, 14332, 12961, 7251, 39237, 12174, 2288, 16606, 2655, 11296, 15844, 1025, 5254, 8717, 7649, 20498, 2655, 2288, 46599, 1863, 4587, 1829, 9381, 23032, 3555, 3615, 995, 14111, 9122, 2304, 10632, 2423, 39184, 47463, 649, 12, 71, 298, 45841, 13496, 13672, 1829, 8032, 2655, 3615, 4117, 3794, 5296, 24793, 10632, 2423, 39184, 3655, 45841, 13496, 8978, 18863, 9957, 3660, 15844, 23032, 12610, 9673, 29245, 1025, 5254, 8717, 7649, 20498, 2655, 2288, 4724, 995, 14851, 9778, 3215, 10943, 10874, 3224, 31377, 2423, 39184, 6177, 11741, 310, 13606], 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1923.82, "end": 1924.42, "word": " أيضًا", "probability": 0.8812255859375}, {"start": 1924.42, "end": 1924.9, "word": " يكون", "probability": 0.98388671875}, {"start": 1924.9, "end": 1925.12, "word": " فيه", "probability": 0.9345703125}, {"start": 1925.12, "end": 1925.74, "word": " homoglobin", "probability": 0.9142252604166666}, {"start": 1925.74, "end": 1926.28, "word": " عشان", "probability": 0.97021484375}, {"start": 1926.28, "end": 1926.8, "word": " يتفاعل", "probability": 0.9826171875}, {"start": 1926.8, "end": 1926.98, "word": " مع", "probability": 0.99462890625}, {"start": 1926.98, "end": 1927.12, "word": " ال", "probability": 0.9921875}, {"start": 1927.12, "end": 1927.64, "word": " reagent", "probability": 0.90185546875}, {"start": 1927.64, "end": 1928.34, "word": " ويعطيهم", "probability": 0.770361328125}, {"start": 1928.34, "end": 1928.46, "word": " ال", "probability": 0.90478515625}, {"start": 1928.46, "end": 1928.96, "word": " cyanomet", "probability": 0.8349609375}, {"start": 1928.96, "end": 1929.58, "word": "-homoglobin", "probability": 0.95439453125}, {"start": 1929.58, "end": 1930.04, "word": " اللي", "probability": 0.974853515625}, {"start": 1930.04, "end": 1930.36, "word": " أيضًا", "probability": 0.962890625}, {"start": 1930.36, "end": 1930.94, "word": " تقاسم", "probability": 0.8116455078125}, {"start": 1930.94, "end": 1931.24, "word": " على", "probability": 0.953125}, {"start": 1931.24, "end": 1932.0, "word": " ال", "probability": 0.92236328125}, {"start": 1932.0, "end": 1934.06, "word": " 540", "probability": 0.73974609375}, {"start": 1934.06, "end": 1934.52, "word": " nanometer", "probability": 0.6845703125}, {"start": 1934.52, "end": 1934.86, "word": " زيها", "probability": 0.9599609375}, {"start": 1934.86, "end": 1935.0, "word": " زي", "probability": 0.9755859375}, {"start": 1935.0, "end": 1935.5, "word": " العينة", "probability": 0.9659830729166666}], "temperature": 1.0}, {"id": 88, "seek": 196204, "start": 1936.12, "end": 1962.04, "text": "تمام؟ اللهم إنه في اختلاف بسيط هنا إنه بدل إطباء طيوب واحدة من ال standard أنا بحضر عدة قنابيب هذه القنابيب فيها طبعا تركيز متدرجة من ال standard من ال homoglobin طبعا هو ال standard محلول homoglobin إذا هتكون فيه تركيز متدرجة من ال homoglobin في هذه التخفيفات من ال standard", "tokens": [39237, 10943, 22807, 13672, 16095, 36145, 3224, 8978, 1975, 46456, 15040, 5172, 4724, 3794, 1829, 9566, 34105, 36145, 3224, 47525, 1211, 11933, 9566, 3555, 16606, 23032, 1829, 37746, 36764, 24401, 3660, 9154, 2423, 3832, 41850, 4724, 5016, 11242, 2288, 6225, 41891, 12174, 8315, 21292, 3555, 29538, 25062, 8315, 21292, 3555, 8978, 11296, 23032, 3555, 3615, 995, 6055, 31747, 1829, 11622, 44650, 3215, 47341, 3660, 9154, 2423, 3832, 9154, 2423, 3655, 45841, 13496, 23032, 3555, 3615, 995, 31439, 2423, 3832, 3714, 5016, 1211, 12610, 3655, 45841, 13496, 11933, 15730, 8032, 2655, 30544, 8978, 3224, 6055, 31747, 1829, 11622, 44650, 3215, 47341, 3660, 9154, 2423, 3655, 45841, 13496, 8978, 29538, 16712, 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0.68011474609375}, {"start": 1941.32, "end": 1941.74, "word": " طيوب", "probability": 0.731201171875}, {"start": 1941.74, "end": 1942.16, "word": " واحدة", "probability": 0.97998046875}, {"start": 1942.16, "end": 1942.3, "word": " من", "probability": 0.99560546875}, {"start": 1942.3, "end": 1942.44, "word": " ال", "probability": 0.9775390625}, {"start": 1942.44, "end": 1942.82, "word": " standard", "probability": 0.74462890625}, {"start": 1942.82, "end": 1943.06, "word": " أنا", "probability": 0.8154296875}, {"start": 1943.06, "end": 1943.6, "word": " بحضر", "probability": 0.9691162109375}, {"start": 1943.6, "end": 1943.92, "word": " عدة", "probability": 0.986572265625}, {"start": 1943.92, "end": 1944.56, "word": " قنابيب", "probability": 0.831787109375}, {"start": 1944.56, "end": 1945.1, "word": " هذه", "probability": 0.34814453125}, {"start": 1945.1, "end": 1945.94, "word": " القنابيب", "probability": 0.94482421875}, {"start": 1945.94, "end": 1946.7, "word": " فيها", "probability": 0.9873046875}, {"start": 1946.7, "end": 1947.32, "word": " طبعا", "probability": 0.9534912109375}, {"start": 1947.32, "end": 1948.48, "word": " تركيز", "probability": 0.9532470703125}, {"start": 1948.48, "end": 1949.66, "word": " متدرجة", "probability": 0.98583984375}, {"start": 1949.66, "end": 1950.26, "word": " من", "probability": 0.99609375}, {"start": 1950.26, "end": 1950.56, "word": " ال", "probability": 0.9765625}, {"start": 1950.56, "end": 1951.0, "word": " standard", "probability": 0.9140625}, {"start": 1951.0, "end": 1951.2, "word": " من", "probability": 0.70556640625}, {"start": 1951.2, "end": 1951.34, "word": " ال", "probability": 0.9931640625}, {"start": 1951.34, "end": 1951.96, "word": " homoglobin", "probability": 0.8548177083333334}, {"start": 1951.96, "end": 1952.88, "word": " طبعا", "probability": 0.9791259765625}, {"start": 1952.88, "end": 1953.08, "word": " هو", "probability": 0.9951171875}, {"start": 1953.08, "end": 1953.26, "word": " ال", "probability": 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"probability": 0.93798828125}, {"start": 1957.88, "end": 1958.16, "word": " هذه", "probability": 0.6982421875}, {"start": 1958.16, "end": 1958.9, "word": " التخفيفات", "probability": 0.89404296875}, {"start": 1958.9, "end": 1959.18, "word": " من", "probability": 0.99169921875}, {"start": 1959.18, "end": 1961.5, "word": " ال", "probability": 0.94140625}, {"start": 1961.5, "end": 1962.04, "word": " standard", "probability": 0.97705078125}], "temperature": 1.0}, {"id": 89, "seek": 198008, "start": 1962.64, "end": 1980.08, "text": "بجيب 6 قناة بيبليك يوم الأولى هحط فيها zero من ال standard و خمسة أمقال من ال Drapkin reagent ليه Drapkin reagent؟ هو عبارة عن إيش؟ هو عبارة عن ال potassium ferricyanide و ال potassium cyanide هدول حكينا إيش اسمهم؟", "tokens": [3555, 7435, 1829, 3555, 1386, 12174, 8315, 3660, 4724, 1829, 3555, 20292, 4117, 7251, 20498, 16247, 12610, 7578, 8032, 5016, 9566, 8978, 11296, 4018, 9154, 2423, 3832, 4032, 16490, 2304, 3794, 3660, 5551, 2304, 4587, 6027, 9154, 2423, 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" هحط", "probability": 0.8009440104166666}, {"start": 1965.12, "end": 1965.4, "word": " فيها", "probability": 0.986083984375}, {"start": 1965.4, "end": 1965.74, "word": " zero", "probability": 0.39599609375}, {"start": 1965.74, "end": 1965.96, "word": " من", "probability": 0.994140625}, {"start": 1965.96, "end": 1966.08, "word": " ال", "probability": 0.97314453125}, {"start": 1966.08, "end": 1966.72, "word": " standard", "probability": 0.91162109375}, {"start": 1966.72, "end": 1967.3, "word": " و", "probability": 0.97802734375}, {"start": 1967.3, "end": 1968.26, "word": " خمسة", "probability": 0.7718505859375}, {"start": 1968.26, "end": 1968.66, "word": " أمقال", "probability": 0.68060302734375}, {"start": 1968.66, "end": 1968.84, "word": " من", "probability": 0.99072265625}, {"start": 1968.84, "end": 1969.02, "word": " ال", "probability": 0.80078125}, {"start": 1969.02, "end": 1969.72, "word": " Drapkin", "probability": 0.5354817708333334}, {"start": 1969.72, "end": 1970.28, "word": " reagent", "probability": 0.790283203125}, {"start": 1970.28, "end": 1970.48, "word": " ليه", "probability": 0.568115234375}, {"start": 1970.48, "end": 1970.84, "word": " Drapkin", "probability": 0.8639322916666666}, {"start": 1970.84, "end": 1971.36, "word": " reagent؟", "probability": 0.7926432291666666}, {"start": 1971.36, "end": 1971.4, "word": " هو", "probability": 0.85009765625}, {"start": 1971.4, "end": 1971.74, "word": " عبارة", "probability": 0.996337890625}, {"start": 1971.74, "end": 1971.9, "word": " عن", "probability": 0.99365234375}, {"start": 1971.9, "end": 1972.68, "word": " إيش؟", "probability": 0.78143310546875}, {"start": 1972.68, "end": 1972.88, "word": " هو", "probability": 0.93359375}, {"start": 1972.88, "end": 1973.96, "word": " عبارة", "probability": 0.9981689453125}, {"start": 1973.96, "end": 1974.4, "word": " عن", "probability": 0.99658203125}, {"start": 1974.4, "end": 1975.2, "word": " ال", "probability": 0.8310546875}, {"start": 1975.2, "end": 1976.0, "word": 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تحفظوها الكلمة لإنها بتضالها مستمرة معاكم كل ما بدك تشتغلي homoglobin أو تشتغلي CBC أو كذا لازم ينذكر ل Drapkin reagent تمام؟", "tokens": [35, 4007, 5843, 26949, 317, 11933, 15730, 2423, 26949, 317, 13672, 1829, 7251, 14851, 9778, 40448, 8978, 6156, 5016, 9381, 2423, 3655, 45841, 13496, 24525, 2304, 3224, 413, 4007, 5843, 26949, 317, 4032, 2304, 16095, 10874, 28259, 6055, 5016, 5172, 19913, 2407, 11296, 33251, 19528, 3660, 5296, 28814, 1863, 11296, 39894, 11242, 6027, 11296, 3714, 14851, 2304, 25720, 20449, 995, 24793, 28242, 19446, 47525, 4117, 6055, 8592, 2655, 17082, 20292, 3655, 45841, 13496, 34051, 6055, 8592, 2655, 17082, 20292, 383, 7869, 34051, 9122, 15730, 5296, 31377, 2304, 7251, 1863, 8848, 37983, 5296, 413, 4007, 5843, 26949, 317, 46811, 10943, 22807], "avg_logprob": -0.18301361268109614, "compression_ratio": 1.6700507614213198, "no_speech_prob": 3.6954879760742188e-06, "words": [{"start": 1980.43, "end": 1981.29, "word": "Drapkin", "probability": 0.7269694010416666}, 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الصدات على فرق الصينة هاي ال slope عبارة عن delta Y على delta X فرق الصدات على فرق الصينة طيب فرق الصدات هو عبارة عن إيش يا بنات؟ هو فرق عن .. فرق absorbance طب absorbance لمين؟", "tokens": [3224, 2407, 38392, 37746, 12602, 4587, 2304, 36764, 24401, 8978, 38392, 37746, 9307, 2423, 3832, 7605, 23032, 1829, 3555, 9154, 2423, 7605, 13672, 1829, 41850, 12602, 38251, 47395, 11331, 4117, 1829, 8315, 36145, 3224, 41850, 4724, 16254, 5551, 7435, 1829, 3555, 13525, 4032, 4386, 13672, 1829, 31439, 6225, 3555, 9640, 3660, 18871, 6156, 2288, 4587, 31767, 3215, 9307, 15844, 6156, 2288, 4587, 31767, 9957, 3660, 8032, 47302, 2423, 13525, 6225, 3555, 9640, 3660, 18871, 8289, 398, 15844, 8289, 1783, 6156, 2288, 4587, 31767, 3215, 9307, 15844, 6156, 2288, 4587, 31767, 9957, 3660, 23032, 1829, 3555, 6156, 2288, 4587, 31767, 3215, 9307, 31439, 6225, 3555, 9640, 3660, 18871, 11933, 1829, 8592, 35186, 4724, 8315, 2655, 22807, 31439, 6156, 2288, 4587, 18871, 4386, 6156, 2288, 4587, 15631, 719, 23032, 3555, 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"end": 2357.29, "word": " فرق", "probability": 0.9959309895833334}, {"start": 2357.29, "end": 2357.89, "word": " الصينة", "probability": 0.9921875}, {"start": 2357.89, "end": 2358.49, "word": " طيب", "probability": 0.8014322916666666}, {"start": 2358.49, "end": 2359.07, "word": " فرق", "probability": 0.9557291666666666}, {"start": 2359.07, "end": 2359.51, "word": " الصدات", "probability": 0.9908854166666666}, {"start": 2359.51, "end": 2359.65, "word": " هو", "probability": 0.8994140625}, {"start": 2359.65, "end": 2359.99, "word": " عبارة", "probability": 0.998779296875}, {"start": 2359.99, "end": 2360.17, "word": " عن", "probability": 0.98486328125}, {"start": 2360.17, "end": 2360.35, "word": " إيش", "probability": 0.8748372395833334}, {"start": 2360.35, "end": 2360.47, "word": " يا", "probability": 0.9833984375}, {"start": 2360.47, "end": 2360.79, "word": " بنات؟", "probability": 0.82763671875}, {"start": 2360.79, "end": 2360.85, "word": " هو", "probability": 0.91845703125}, {"start": 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240429, "start": 2382.41, "end": 2404.29, "text": "طيب فرق الصينات عبارة عن إيش؟ فرق ال concentration طب ال concentration هذا لمين؟ لل standard إذا برضه كمان فرق ال concentration لل standard إذا بإمكاني أنسى delta Y على delta X و أحكي إنه ال slope هو عبارة عن ال absorbance لل standard، هو عبارة عن قيمة absorbance أو فرق absorbance", "tokens": [9566, 1829, 3555, 6156, 2288, 4587, 31767, 1829, 8315, 2655, 6225, 3555, 9640, 3660, 18871, 11933, 1829, 8592, 22807, 6156, 2288, 4587, 2423, 9856, 23032, 3555, 2423, 9856, 23758, 5296, 2304, 9957, 22807, 24976, 3832, 11933, 15730, 4724, 43042, 3224, 9122, 2304, 7649, 6156, 2288, 4587, 2423, 9856, 24976, 3832, 11933, 15730, 4724, 28814, 2304, 41361, 1829, 14739, 3794, 7578, 8289, 398, 15844, 8289, 1783, 4032, 5551, 5016, 4117, 1829, 36145, 3224, 2423, 13525, 31439, 6225, 3555, 9640, 3660, 18871, 2423, 15631, 719, 24976, 3832, 12399, 31439, 6225, 3555, 9640, 3660, 18871, 12174, 32640, 3660, 15631, 719, 34051, 6156, 2288, 4587, 15631, 719], 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أطائي clear صح هو ملون و أنا بدي أقيس شدة لون على الجهاز بس لازم يكون", "tokens": [10721, 1829, 11242, 14111, 995, 9122, 2304, 7649, 8978, 18871, 16254, 5551, 2304, 13063, 7251, 26108, 3555, 3714, 23557, 3615, 995, 34268, 11296, 5551, 12984, 1863, 16606, 11933, 7435, 2288, 16606, 27188, 5016, 9381, 30767, 1829, 50113, 1211, 14111, 995, 14739, 3224, 9673, 5016, 1211, 12610, 13672, 1829, 41850, 4724, 8592, 2655, 17082, 1211, 47356, 34051, 13672, 1829, 4724, 16254, 5551, 38436, 3794, 3224, 7251, 30544, 5551, 9566, 16373, 1829, 1850, 20328, 5016, 31439, 3714, 1211, 11536, 4032, 41850, 4724, 16254, 5551, 38436, 3794, 13412, 41891, 5296, 11536, 15844, 25724, 3224, 31377, 4724, 3794, 5296, 31377, 2304, 7251, 30544], "avg_logprob": -0.13210937932133673, "compression_ratio": 1.702020202020202, "no_speech_prob": 4.708766937255859e-06, "words": [{"start": 2792.81, "end": 2793.75, "word": "أيضًا", "probability": 0.777978515625}, {"start": 2793.75, "end": 2794.47, "word": " كمان", "probability": 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3295.06, "word": " ال", "probability": 0.9921875}, {"start": 3295.06, "end": 3296.24, "word": " acid", "probability": 0.68115234375}, {"start": 3296.24, "end": 3296.38, "word": " و", "probability": 0.98779296875}, {"start": 3296.38, "end": 3296.82, "word": " يكونلي", "probability": 0.7893880208333334}, {"start": 3296.82, "end": 3297.2, "word": " hydrogen", "probability": 0.9150390625}, {"start": 3297.2, "end": 3297.78, "word": " cyanide", "probability": 0.952880859375}, {"start": 3297.78, "end": 3298.06, "word": " gas", "probability": 0.712890625}, {"start": 3298.06, "end": 3298.2, "word": " اللى", "probability": 0.789794921875}, {"start": 3298.2, "end": 3298.34, "word": " هو", "probability": 0.9052734375}, {"start": 3298.34, "end": 3298.64, "word": " toxic", "probability": 0.966796875}, {"start": 3298.64, "end": 3299.04, "word": " gas", "probability": 0.94091796875}], "temperature": 1.0}, {"id": 150, "seek": 332269, "start": 3299.57, "end": 3322.69, "text": "تمام؟ إذا هتلاقوني هتلاحظوا عليا إنه أول ما بدتوا تطلفوا أو إنه تعملوا discard لمحتوى القنابيب تلاقوني فاتحت الحنافية و سبتها مفتوحة ماحدش يسكرها لإنها بديها تعمل dilution لها هذا ال agent بحيث إنه ما يكون عم بنشكل أو بننتج غاز سام إنه بعد هيك أي حد يفوت على المختبر يتأذى", "tokens": [39237, 10943, 22807, 11933, 15730, 8032, 2655, 15040, 4587, 11536, 1829, 8032, 2655, 15040, 5016, 19913, 14407, 11203, 25528, 36145, 3224, 5551, 12610, 19446, 47525, 2655, 14407, 6055, 9566, 46538, 14407, 34051, 36145, 3224, 6055, 25957, 1211, 14407, 31597, 32767, 33753, 2407, 7578, 25062, 8315, 21292, 3555, 6055, 15040, 4587, 11536, 1829, 6156, 9307, 33753, 21542, 8315, 5172, 10632, 4032, 8608, 3555, 2655, 11296, 3714, 5172, 2655, 2407, 5016, 3660, 19446, 24401, 8592, 7251, 3794, 37983, 11296, 5296, 28814, 1863, 11296, 4724, 16254, 11296, 6055, 25957, 1211, 11504, 1448, 5296, 11296, 23758, 2423, 9461, 4724, 5016, 1829, 12984, 36145, 3224, 19446, 7251, 30544, 6225, 2304, 44945, 8592, 28820, 34051, 44945, 29399, 7435, 32771, 31377, 8608, 10943, 36145, 3224, 39182, 39896, 4117, 36632, 11331, 3215, 7251, 5172, 35473, 15844, 9673, 46456, 26890, 7251, 2655, 10721, 8848, 7578], "avg_logprob": -0.15659216024579792, "compression_ratio": 1.7555555555555555, "no_speech_prob": 3.933906555175781e-06, "words": [{"start": 3299.57, "end": 3300.43, "word": "تمام؟", "probability": 0.8253580729166666}, {"start": 3300.43, "end": 3300.69, "word": " إذا", "probability": 0.640380859375}, {"start": 3300.69, "end": 3301.29, "word": " هتلاقوني", "probability": 0.8607584635416666}, {"start": 3301.29, "end": 3301.85, "word": " هتلاحظوا", "probability": 0.9388834635416666}, {"start": 3301.85, "end": 3302.11, "word": " عليا", "probability": 0.7191162109375}, {"start": 3302.11, "end": 3302.31, "word": " إنه", "probability": 0.7255859375}, {"start": 3302.31, "end": 3302.57, "word": " أول", "probability": 0.939453125}, {"start": 3302.57, "end": 3302.69, "word": " ما", "probability": 0.8388671875}, {"start": 3302.69, "end": 3303.05, "word": " بدتوا", "probability": 0.83251953125}, {"start": 3303.05, "end": 3303.63, "word": " تطلفوا", "probability": 0.90380859375}, {"start": 3303.63, "end": 3303.93, "word": " أو", "probability": 0.912109375}, {"start": 3303.93, "end": 3304.35, "word": " إنه", "probability": 0.7119140625}, {"start": 3304.35, "end": 3304.71, "word": " تعملوا", "probability": 0.9627685546875}, {"start": 3304.71, "end": 3305.23, "word": " discard", "probability": 0.96142578125}, {"start": 3305.23, "end": 3305.85, "word": " لمحتوى", "probability": 0.9140625}, {"start": 3305.85, "end": 3306.39, "word": " القنابيب", "probability": 0.9039306640625}, {"start": 3306.39, "end": 3306.99, "word": " تلاقوني", "probability": 0.8171142578125}, {"start": 3306.99, "end": 3307.35, "word": " فاتحت", "probability": 0.8504231770833334}, {"start": 3307.35, "end": 3307.83, "word": " الحنافية", "probability": 0.8782958984375}, {"start": 3307.83, "end": 3307.95, "word": " و", "probability": 0.92626953125}, {"start": 3307.95, "end": 3308.21, "word": " سبتها", "probability": 0.8594970703125}, {"start": 3308.21, "end": 3308.81, "word": " مفتوحة", "probability": 0.985595703125}, {"start": 3308.81, "end": 3309.53, "word": " ماحدش", "probability": 0.8623046875}, {"start": 3309.53, "end": 3310.27, "word": " يسكرها", "probability": 0.97265625}, {"start": 3310.27, "end": 3311.01, "word": " لإنها", "probability": 0.905029296875}, {"start": 3311.01, "end": 3311.47, "word": " بديها", "probability": 0.7401529947916666}, {"start": 3311.47, "end": 3311.81, "word": " تعمل", "probability": 0.98388671875}, {"start": 3311.81, "end": 3312.35, "word": " dilution", "probability": 0.9365234375}, {"start": 3312.35, "end": 3312.73, "word": " لها", "probability": 0.78125}, {"start": 3312.73, "end": 3312.99, "word": " هذا", "probability": 0.8671875}, {"start": 3312.99, "end": 3313.09, "word": " ال", "probability": 0.96533203125}, {"start": 3313.09, "end": 3313.51, "word": " agent", "probability": 0.78759765625}, {"start": 3313.51, "end": 3314.29, "word": " بحيث", "probability": 0.9552001953125}, {"start": 3314.29, "end": 3315.05, "word": " إنه", "probability": 0.905517578125}, {"start": 3315.05, "end": 3315.31, "word": " ما", "probability": 0.98388671875}, {"start": 3315.31, "end": 3315.69, "word": " يكون", "probability": 0.71533203125}, {"start": 3315.69, "end": 3315.97, "word": " عم", "probability": 0.89208984375}, {"start": 3315.97, "end": 3317.15, "word": " بنشكل", "probability": 0.9676106770833334}, {"start": 3317.15, "end": 3317.43, "word": " أو", "probability": 0.87158203125}, {"start": 3317.43, "end": 3319.03, "word": " بننتج", "probability": 0.71142578125}, {"start": 3319.03, "end": 3319.37, "word": " غاز", "probability": 0.9716796875}, {"start": 3319.37, "end": 3319.77, "word": " سام", "probability": 0.969482421875}, {"start": 3319.77, "end": 3320.37, "word": " إنه", "probability": 0.85888671875}, {"start": 3320.37, "end": 3320.57, "word": " بعد", "probability": 0.9404296875}, {"start": 3320.57, "end": 3320.77, "word": " هيك", "probability": 0.486572265625}, {"start": 3320.77, "end": 3320.87, "word": " أي", "probability": 0.63720703125}, {"start": 3320.87, "end": 3321.07, "word": " حد", "probability": 0.786865234375}, {"start": 3321.07, "end": 3321.47, "word": " يفوت", "probability": 0.87548828125}, {"start": 3321.47, "end": 3321.61, "word": " على", "probability": 0.8291015625}, {"start": 3321.61, "end": 3322.07, "word": " المختبر", "probability": 0.9835611979166666}, {"start": 3322.07, "end": 3322.69, "word": " يتأذى", "probability": 0.92607421875}], "temperature": 1.0}, {"id": 151, "seek": 333036, "start": 3324.74, "end": 3330.36, "text": "هك بنكون انتهانا من محاضرتنا لليوم يعطيكم ألف عافية ونراكم في المعمل للتطبيق", "tokens": [3224, 4117, 44945, 30544, 16472, 47395, 7649, 995, 9154, 3714, 5016, 46958, 43500, 8315, 5296, 20292, 20498, 37495, 9566, 1829, 24793, 5551, 46538, 6225, 31845, 10632, 4032, 1863, 23557, 24793, 8978, 9673, 25957, 1211, 24976, 2655, 9566, 21292, 4587], "avg_logprob": -0.09687500149011612, "compression_ratio": 1.386138613861386, "no_speech_prob": 0.0, "words": [{"start": 3324.74, "end": 3325.02, "word": "هك", "probability": 0.915771484375}, {"start": 3325.02, "end": 3325.38, "word": " بنكون", "probability": 0.955322265625}, {"start": 3325.38, "end": 3326.0, "word": " انتهانا", "probability": 0.8880615234375}, {"start": 3326.0, "end": 3326.14, "word": " من", "probability": 0.9833984375}, {"start": 3326.14, "end": 3326.76, "word": " محاضرتنا", "probability": 0.96865234375}, {"start": 3326.76, "end": 3327.22, "word": " لليوم", "probability": 0.9182942708333334}, {"start": 3327.22, "end": 3327.72, "word": " يعطيكم", "probability": 0.8431396484375}, {"start": 3327.72, "end": 3327.96, "word": " ألف", "probability": 0.743408203125}, {"start": 3327.96, "end": 3328.38, "word": " عافية", "probability": 0.9747721354166666}, {"start": 3328.38, "end": 3328.84, "word": " ونراكم", "probability": 0.8902587890625}, {"start": 3328.84, "end": 3329.1, "word": " في", "probability": 0.96240234375}, {"start": 3329.1, "end": 3329.7, "word": " المعمل", "probability": 0.9680989583333334}, {"start": 3329.7, "end": 3330.36, "word": " للتطبيق", "probability": 0.9478515625}], "temperature": 1.0}], "language": "ar", "language_probability": 1.0, "duration": 3334.85, "duration_after_vad": 3257.005624999992}
\ No newline at end of file
diff --git a/PL9fwy3NUQKwY9jl8xw-bWkVZkWcGGyspd/ZZ8KPmQPnAc_raw.srt b/PL9fwy3NUQKwY9jl8xw-bWkVZkWcGGyspd/ZZ8KPmQPnAc_raw.srt
new file mode 100644
index 0000000000000000000000000000000000000000..c2abe569f05a97a418311cb33d32ce21f82b3a72
--- /dev/null
+++ b/PL9fwy3NUQKwY9jl8xw-bWkVZkWcGGyspd/ZZ8KPmQPnAc_raw.srt
@@ -0,0 +1,2900 @@
+1
+00:00:05,360 --> 00:00:08,860
+السلام عليكم ورحمة الله وبركاته طالبات العزيزات
+
+2
+00:00:08,860 --> 00:00:11,340
+اليوم ان شاء الله راح نشرح homoglobin
+
+3
+00:00:11,340 --> 00:00:14,260
+concentration determination lecture راح نكون مع
+
+4
+00:00:14,260 --> 00:00:17,920
+بعض في محاضرة بعنوان homoglobin concentration
+
+5
+00:00:17,920 --> 00:00:22,940
+determination اللي هو يعني معرفة او قياس تركيز ال
+
+6
+00:00:22,940 --> 00:00:27,140
+homoglobin و راح نتعرف كيف احنا ممكن نقيس نسبة ال
+
+7
+00:00:27,140 --> 00:00:31,480
+homoglobin في الدم وبطريقة او بتطبيق ال beer
+
+8
+00:00:31,480 --> 00:00:37,970
+lambert law homoglobin HPهذا ال HP هو عبارة عن the
+
+9
+00:00:37,970 --> 00:00:43,070
+standard abbreviation for همoglobin هو الإختصار
+
+10
+00:00:43,070 --> 00:00:47,310
+اللي متعارف عليه لبروتين الهمoglobin سواء حكيتلك
+
+11
+00:00:47,310 --> 00:00:51,350
+hemoglobin أو كتبتلك HP فأنا بكون بقصد ال
+
+12
+00:00:51,350 --> 00:00:56,070
+hemoglobin البروتين ال hemoglobin هذا هو عبارة عن
+
+13
+00:00:56,070 --> 00:01:01,430
+ال oxygen carrying pigmentهو الناقل للأكسوجين،
+
+14
+00:01:01,430 --> 00:01:05,510
+تمام؟ هو اللي بيحمل الأكسوجين داخل خلايا ال RBCs و
+
+15
+00:01:05,510 --> 00:01:09,850
+هو ال pigment .. التركيبة ال .. ال homoglobin
+
+16
+00:01:09,850 --> 00:01:15,130
+تعطيه لون أحمر، إذا هو اللي يكسب خلايا الدم
+
+17
+00:01:15,130 --> 00:01:19,590
+الحمراء لونها الأحمر و من هنا إجت تسمية ال RBCs
+
+18
+00:01:19,590 --> 00:01:24,030
+بال red blood cells، تمام؟ من لونها، من لون ال
+
+19
+00:01:24,030 --> 00:01:28,690
+homoglobin فيهالأن هذا ال homoglobin هو ال breed
+
+20
+00:01:28,690 --> 00:01:32,710
+dominant protein هو ال protein الأكثر شيوعا و
+
+21
+00:01:32,710 --> 00:01:38,370
+المحتل أكبر حجم من خلايا ال RBCs أكثر من 60% من
+
+22
+00:01:38,370 --> 00:01:44,950
+حجم ال RBCs هو عبارة عن homoglobin تمام؟ هو عبارة
+
+23
+00:01:44,950 --> 00:01:48,690
+عن homoglobin homoglobin carries oxygen from
+
+24
+00:01:48,690 --> 00:01:53,580
+places of high oxygen pressure lungsto places of
+
+25
+00:01:53,580 --> 00:01:57,660
+low oxygen pressure such as tissues ال homoglobin
+
+26
+00:01:57,660 --> 00:02:03,240
+حكينا إيش وظيفته؟ هو عبارة عن ناقل لأل أكسوجين،
+
+27
+00:02:03,240 --> 00:02:08,560
+إذا هو أثناء سرايان الدم و يذهبوا لال lungs،
+
+28
+00:02:08,560 --> 00:02:12,900
+للريقتين، في الريقتين موجود ضغط عالي من الأكسوجين،
+
+29
+00:02:12,900 --> 00:02:15,920
+تمام؟ لإن الريقتين هي اللي بتستلم الأكسوجين، نعمل
+
+30
+00:02:15,920 --> 00:02:20,100
+إحنا تنفس، شاهيكالأكسوجين بيدخل إلى الرئتين، اذاً
+
+31
+00:02:20,100 --> 00:02:23,440
+الرئتين فيها high oxygen pressure ساعتها
+
+32
+00:02:23,440 --> 00:02:26,120
+الهمoglobin لما يكون عند الرئتين أو يكون في
+
+33
+00:02:26,120 --> 00:02:31,720
+الرئتين إيش راح يعمل؟ راح يبدأ يحمل أكسوجين، تمام؟
+
+34
+00:02:31,720 --> 00:02:36,560
+الهمoglobin راح يحمل أكسوجين في الرئتين ولمّا يعمل
+
+35
+00:02:36,560 --> 00:02:41,180
+circulation هذا الهمoglobin المحمل بالأكسوجينو يصل
+
+36
+00:02:41,180 --> 00:02:46,100
+ال tissue أو ال organs أعضاء الجسم أو أنسجة الجسم
+
+37
+00:02:46,100 --> 00:02:50,440
+اللى عندها low oxygen pressure مافيش عندها oxygen
+
+38
+00:02:50,440 --> 00:02:54,780
+كفاية اش بيروح بيعمل بيبدأ يترك هذا ال oxygen اللى
+
+39
+00:02:54,780 --> 00:02:59,720
+حمله إذا ال homoglobin هي هيك خواص عمله في ال high
+
+40
+00:02:59,720 --> 00:03:06,710
+oxygen pressure بيبدأ يستلم يحمل oxygen تمام؟و في
+
+41
+00:03:06,710 --> 00:03:10,210
+ال low oxygen pressure عند ال tissue بيبدأ يترك
+
+42
+00:03:10,210 --> 00:03:14,530
+oxygen عشان هيك بنحكي عنه ناقل career، delivery
+
+43
+00:03:14,530 --> 00:03:21,250
+لال oxygenHomoglobin also returns CO2 from the
+
+44
+00:03:21,250 --> 00:03:25,890
+tissue to the lung فالمقابل هذا ال homoglobin بي
+
+45
+00:03:25,890 --> 00:03:31,290
+.. مقابل انه بيعطي ال tissue اكسوجين هو يحمل ال
+
+46
+00:03:31,290 --> 00:03:36,330
+CO2 من ال tissue و بيرجعها لل lung علشان تخرج في
+
+47
+00:03:36,330 --> 00:03:41,630
+عملية الزفير هذا ال homoglobin molecule يتكون من
+
+48
+00:03:41,630 --> 00:03:49,150
+جزئين شق بروتيني بنسميه globinهو شق نن بروتين
+
+49
+00:03:49,150 --> 00:03:54,270
+بنسمي الهيم تمام؟ عشان هيك هو اسمه hemoglobin
+
+50
+00:03:54,270 --> 00:03:59,970
+تمام؟ شق بروتيني الoglobin وشق نن بروتين اللي هو
+
+51
+00:03:59,970 --> 00:04:05,550
+الهيم هذا الهيم عبارة عن أربع حلقات من الهيم أربع
+
+52
+00:04:05,550 --> 00:04:11,370
+حلقات هيم كل واحدة منهم مرتبطة مع جزاء iron أو ذرة
+
+53
+00:04:11,370 --> 00:04:16,600
+iron iron تمام؟في الferrocystate إيش يعني في
+
+54
+00:04:16,600 --> 00:04:23,160
+الferrocystate؟ يعني iron Fe plus 2 شحنتهاو هنا
+
+55
+00:04:23,160 --> 00:04:26,840
+حاكي .. حاكيلي one molecule of globin جزء globin
+
+56
+00:04:26,840 --> 00:04:31,160
+معبر عن كل بروتين يعني على كل الشكل البروتيني عبّر
+
+57
+00:04:31,160 --> 00:04:36,220
+عنه بإن هو ال globin part تمام؟ ولكن هذا ال globin
+
+58
+00:04:36,220 --> 00:04:42,560
+part مكوّن من أربع سلاسل ببتيدية four polypeptide
+
+59
+00:04:42,560 --> 00:04:48,380
+chain تمام؟ تنتين منهم من نوع alpha chain وتنتين
+
+60
+00:04:48,380 --> 00:04:53,600
+من نوع beta chainهذه السلاسل الـ alpha chain
+
+61
+00:04:53,600 --> 00:04:57,840
+الواحدة منهم طولها 141 amino acid و ال beta
+
+62
+00:04:57,840 --> 00:05:03,980
+الواحدة منهم 146 amino acidتمام؟ طيب خلينا بس نشوف
+
+63
+00:05:03,980 --> 00:05:07,740
+مع بعض كيف هو تركيب ال homoglobin ال homoglobin
+
+64
+00:05:07,740 --> 00:05:11,060
+مكوّن من ال globin part اللي هو ال protein part
+
+65
+00:05:11,060 --> 00:05:16,360
+الجزء ال protein منه و ال hem part الحلقات ال hem
+
+66
+00:05:16,360 --> 00:05:20,900
+الأربعة اللي هي non-protein part مش .. التركيبها
+
+67
+00:05:20,900 --> 00:05:23,460
+مش protein يعني مافيش فيها peptide bond مافيش فيها
+
+68
+00:05:23,460 --> 00:05:29,030
+amino acids يعني عشان نحكي عنها بروتينيةتمام؟ طيب
+
+69
+00:05:29,030 --> 00:05:35,090
+هذا الoglobin part مكون من 4 سلاسل تنتين alpha
+
+70
+00:05:35,090 --> 00:05:40,310
+مقابل بعضهم البعض و تنتين beta تنتين alpha و تنتين
+
+71
+00:05:40,310 --> 00:05:47,170
+beta بهذا الشكل ال alpha الواحدة منهم طولها 141
+
+72
+00:05:47,170 --> 00:05:53,910
+amino acid ال beta طولها 146 amino acidوكل سلسلة
+
+73
+00:05:53,910 --> 00:05:58,170
+منها دول بتكون الواحدة منهم مرتبطة بحلقة heme
+
+74
+00:05:58,170 --> 00:06:04,790
+تمام؟ حلقة heme حلقة ال heme هذه في مركزها موجود
+
+75
+00:06:04,790 --> 00:06:09,810
+ال iron تمام؟ بالferrous state اللي هو iron Fe
+
+76
+00:06:09,810 --> 00:06:16,570
+plus 2 بيكون طبعا هذا .. عند حلقة ال iron هاي أو
+
+77
+00:06:16,570 --> 00:06:20,800
+حلقة ال heme اللي في مركزها ironهنا يرتبط ال
+
+78
+00:06:20,800 --> 00:06:25,260
+oxygen .. هنا يحمل ال oxygen على ال homoglobin
+
+79
+00:06:25,260 --> 00:06:30,540
+تمام؟ و بالمقابل طبعا في ال lung يحمل ال oxygen و
+
+80
+00:06:30,540 --> 00:06:35,300
+يتم تركه في ال tissue و .. و يحمل بداله مين CO2
+
+81
+00:06:35,300 --> 00:06:40,420
+عشان يرجع .. يرجعه على ال lung و يتم التخلص منه في
+
+82
+00:06:40,420 --> 00:06:46,870
+عملية الزفيرةa heme group is a flat ring molecule
+
+83
+00:06:46,870 --> 00:06:52,110
+containing carbon, nitrogen and hydrogen and
+
+84
+00:06:52,110 --> 00:06:57,130
+oxygen تمام؟ ال heme هي عبارة عن حلقة مسطحة من ال
+
+85
+00:06:57,130 --> 00:07:00,410
+hydrogen و ال carbon و ال .. و ال nitrogen مرتبطة
+
+86
+00:07:00,410 --> 00:07:03,590
+مع بعضها البعض و في وجود أيضا لل oxygen و هي
+
+87
+00:07:03,590 --> 00:07:09,330
+توسطها موجود عندي Fe plus 2 في الرأس molecule وين؟
+
+88
+00:07:09,330 --> 00:07:14,190
+في ال center تمام؟ في ال centerwithout the iron
+
+89
+00:07:14,190 --> 00:07:18,070
+بدون ال iron هذا نخب ال iron شوية without the iron
+
+90
+00:07:18,070 --> 00:07:23,130
+هاي الحلقة بنسميها porphyrin ring مع ال iron بتصير
+
+91
+00:07:23,130 --> 00:07:30,750
+heme without iron porphyrin with iron heme تمام؟
+
+92
+00:07:30,750 --> 00:07:35,370
+فلازم انه نعرف هاي الأساسيات عن تركيب ال
+
+93
+00:07:35,370 --> 00:07:41,030
+homoglobinnormal ranges المدى الطبيعي أو المستوى
+
+94
+00:07:41,030 --> 00:07:46,290
+الطبيعي لأ ال homoglobin طبعا احنا بنقيس ال
+
+95
+00:07:46,290 --> 00:07:50,250
+homoglobin في جسمنا سواء كان انا بحكيلك فحص
+
+96
+00:07:50,250 --> 00:07:55,350
+homoglobin او فحص total homoglobin ف it's the same
+
+97
+00:07:55,350 --> 00:07:58,850
+نفس المعنى طب ايش يعني total homoglobin؟ طب ليش
+
+98
+00:07:58,850 --> 00:08:04,520
+احنا اضفنا كلمة total قبل homoglobin؟لأن يا بنات
+
+99
+00:08:04,520 --> 00:08:10,360
+أنواع ال homoglobin في خلايا ال RBCs مش واحدة،
+
+100
+00:08:10,360 --> 00:08:15,480
+تمام؟ شوفوا معايا، تتذكروا احنا ذكرنا معلومة سابقا
+
+101
+00:08:15,480 --> 00:08:21,730
+حكينا انهالأطفال الحديثي الولادة بيكون عندهم نوع
+
+102
+00:08:21,730 --> 00:08:25,410
+ال homoglobin اللي له high affinity مع ال oxygen
+
+103
+00:08:25,410 --> 00:08:29,270
+عشان هيك مش بسهولة بيترك ال oxygen عند ال tissue
+
+104
+00:08:29,270 --> 00:08:32,890
+عشان هيك ربنا عوضهم بنسبة RBCs ونسبة homoglobin
+
+105
+00:08:32,890 --> 00:08:37,390
+عالية بالاضافة لإنه أيضا عندهم growth pairs طيب
+
+106
+00:08:37,390 --> 00:08:41,090
+هدول حكيتلكم عندهم النوع ال homoglobin F
+
+107
+00:08:41,090 --> 00:08:46,850
+homoglobin F اللي هو ال fetal homoglobinأطفال حدثي
+
+108
+00:08:46,850 --> 00:08:49,830
+الولادة عندهم فيتا الهموجلبيين هو الشائع في ال
+
+109
+00:08:49,830 --> 00:08:56,790
+RBCs طيب بيبدأ .. بيبدوا يكبروا هدول الناس الأطفال
+
+110
+00:08:56,790 --> 00:09:01,950
+حدثي الولادة وبيبدأ مستوى الهموجلبيين يقل عندهم
+
+111
+00:09:02,210 --> 00:09:06,370
+ليش؟ لإنه بيصير فيه تغيير في .. في تركيب ال fetal
+
+112
+00:09:06,370 --> 00:09:10,830
+homoglobin هذا وبيبدأ يصير اتقل التركيب الجديد
+
+113
+00:09:10,830 --> 00:09:15,890
+اللي بينتج أو التغيير على تركيبه هذا بينتج منه
+
+114
+00:09:15,890 --> 00:09:21,140
+homoglobin ال affinity لل oxygen تبعته معتدلهيعني
+
+115
+00:09:21,140 --> 00:09:25,920
+بيصير عادي بسهولة يترك ال oxygen عند ال tissue
+
+116
+00:09:25,920 --> 00:09:29,960
+طبعا هذا ال homoglobin بنسميه ال adult homoglobin
+
+117
+00:09:29,960 --> 00:09:34,780
+اللي هو و نسبته تقريبا 95% من خلايانا احنا ك adult
+
+118
+00:09:34,780 --> 00:09:38,880
+كناس .. كناس مش حدثي الولادة يعني الأطفال و ال
+
+119
+00:09:38,880 --> 00:09:44,420
+adult و .. يعني اللي أكبر من شهر تقريبا أو أكبر من
+
+120
+00:09:44,420 --> 00:09:50,640
+شهرين فهدول بيكون عندهم نسبة ال homoglobinال .. ال
+
+121
+00:09:50,640 --> 00:09:53,300
+.. المعتدلة affinity تبعته، ال affinity تبعته
+
+122
+00:09:53,300 --> 00:09:57,420
+معتدلة بتكون عالية و هذا ال homoglobin بسميه
+
+123
+00:09:57,420 --> 00:10:04,700
+homoglobin A و نسبته 95% عندنا كناس adult، تمام؟
+
+124
+00:10:04,700 --> 00:10:07,810
+طيبفي عندي أنواع أخرى طبعا يعني أنا لما بحكي أنه
+
+125
+00:10:07,810 --> 00:10:12,990
+أنا في خلايانا في 95% أو أكثر من ال homoglobin A
+
+126
+00:10:12,990 --> 00:10:16,350
+فبيكون في عندي نسب ضئيلة لاتكاد تذكر من ال
+
+127
+00:10:16,350 --> 00:10:20,830
+homoglobin F و ال homoglobin A2 تمام؟ نسب ضئيلة من
+
+128
+00:10:20,830 --> 00:10:24,890
+homoglobin F and homoglobin A2 عشان هيك لما أجي
+
+129
+00:10:24,890 --> 00:10:29,950
+أحكي أنا بعمل فحص لأ ال total homoglobin أنا بكون
+
+130
+00:10:29,950 --> 00:10:34,280
+عم بذكر ال homoglobin Aاللي هو ال adult homoglobin
+
+131
+00:10:34,280 --> 00:10:37,980
+ال homoglobin A2 و ال homoglobin F طب بقاشى
+
+132
+00:10:37,980 --> 00:10:41,240
+بيختلفوا هدول الأنواع و ليش احنا هيك بنسميهم مش
+
+133
+00:10:41,240 --> 00:10:45,400
+كلهم homoglobin و السلام لإنهم بيختلفوا بتركيبهم
+
+134
+00:10:45,400 --> 00:10:50,280
+بيختلفوا بالoglobin part تبعهم مش طبعا ال alpha
+
+135
+00:10:50,280 --> 00:10:54,160
+homoglobin او sorry ال homoglobin A اللي هو ال
+
+136
+00:10:54,160 --> 00:10:57,760
+adult homoglobin هذا هو اللي بيتكون من تنتين alpha
+
+137
+00:10:57,760 --> 00:11:01,650
+تنتين beta chainsلكن ال homoglobin A2 و ال
+
+138
+00:11:01,650 --> 00:11:06,330
+homoglobin F تركيبهم مختلف شوية عشان هيك احنا ..
+
+139
+00:11:06,980 --> 00:11:12,440
+بنصنفهم يعني هذا لأ هذا A هذا A2 هذا F حسب تركيب
+
+140
+00:11:12,440 --> 00:11:18,020
+الجلبين part تبعهم تمام اللي هم الجلبين A مكوّن من
+
+141
+00:11:18,020 --> 00:11:23,260
+تنتين Alpha وتنتين Beta لكن هم الجلبين A2 مكوّن من
+
+142
+00:11:23,260 --> 00:11:27,820
+سلاسل أخرى راح تتعرفوا عليها بمساقل hematology لما
+
+143
+00:11:27,820 --> 00:11:32,030
+تصيروا سنة تالتة ان شاء اللهطبعا فإذا هذا يعني
+
+144
+00:11:32,030 --> 00:11:35,550
+إحنا ليش ذكرنا هذه المعلومات علشان بس نحكي أنه لما
+
+145
+00:11:35,550 --> 00:11:39,430
+أنا بقيس ال homoglobin بقيس total homoglobin إيش
+
+146
+00:11:39,430 --> 00:11:42,650
+يعني total homoglobin؟ يعني كل أنواع ال homoglobin
+
+147
+00:11:42,650 --> 00:11:46,090
+اللي موجودة في ال RBCs ليش؟ هو في أنواع؟ أه في
+
+148
+00:11:46,090 --> 00:11:50,570
+أنواع95% منهم الـ adult صح هو الأكثر شيوعا هو
+
+149
+00:11:50,570 --> 00:11:53,450
+المكون من تنتين Alpha تنتين Beta عندنا ك adult
+
+150
+00:11:53,450 --> 00:11:58,770
+تمام؟ ولكن في عندنا لسه ضايق جزء من اللي هو ال
+
+151
+00:11:58,770 --> 00:12:01,510
+homoglobin اللي كان عندنا في ولادها homoglobin F
+
+152
+00:12:01,510 --> 00:12:05,270
+وعندنا جزء ضئيل أيضا من نوع آخر اسمه homoglobin A2
+
+153
+00:12:05,270 --> 00:12:09,790
+تمام؟ عشان هيك لما أحكيلك نقيس ال homoglobin أو ال
+
+154
+00:12:09,790 --> 00:12:14,000
+total homoglobin it's the same نفس المعنىطيب
+
+155
+00:12:14,000 --> 00:12:18,160
+الوحدة اللي أنا بعبر عنها لقياس ال homoglobin هي
+
+156
+00:12:18,160 --> 00:12:22,780
+gram per deciliter تمام؟ gram per deciliter
+
+157
+00:12:22,780 --> 00:12:27,740
+تتذكروا لما شرحنا فحص السكر حكينا إنه أنا بقيسه
+
+158
+00:12:27,740 --> 00:12:31,160
+بال milligram per deciliter طيب ليش بقيسه بال
+
+159
+00:12:31,160 --> 00:12:34,120
+milligram per deciliter و البروتين بال gram per
+
+160
+00:12:34,120 --> 00:12:39,540
+deciliter؟ لإنه احنا كطبيعة عندنا نسبة البروتين
+
+161
+00:12:39,540 --> 00:12:42,680
+أعلى من النسب الأخرى من السكر و الأشياء التانية
+
+162
+00:12:43,120 --> 00:12:48,180
+فبالتالي البروتين نظرا لو وجوده بتركيز أعلى فإذا
+
+163
+00:12:48,180 --> 00:12:52,860
+وحدة قياسه اجرام per deciliter وليس ميلي جرام per
+
+164
+00:12:52,860 --> 00:12:57,500
+deciliter the normal ranges of homoglobin depend
+
+165
+00:12:57,500 --> 00:13:01,440
+on طبعا المدى الطبيعي للهومoglobin بيعتمد على عدة
+
+166
+00:13:01,440 --> 00:13:06,120
+أسباب أو عدة عوامل إيش هي هذه العوامل ال edgeلسه
+
+167
+00:13:06,120 --> 00:13:09,560
+.. لسه من شويه ذكرنا انه الأطفال حديثي الولادة
+
+168
+00:13:09,560 --> 00:13:12,700
+بيكون عندهم نسبة homoglobin أعلى لما بيبدوا يكبر
+
+169
+00:13:12,700 --> 00:13:15,980
+شويه بتبدأ يتقل نسبة ال homoglobin لإنه اتغير
+
+170
+00:13:15,980 --> 00:13:20,420
+تركيبه فالتركيب الجديد اللي صدر صار عنده أقل
+
+171
+00:13:20,420 --> 00:13:24,140
+affinity لال oxygen فصار مافيهم مش في حاجة لإنه
+
+172
+00:13:24,140 --> 00:13:32,040
+يتصنعلهم homoglobin أكتر تمام؟ و ال attitude .. ال
+
+173
+00:13:32,040 --> 00:13:37,850
+altitudeالارتفاع، ناس عايش على مرتفعات عندها نقص
+
+174
+00:13:37,850 --> 00:13:41,930
+في الأكسجين، إذا جسمها هيعود بإنتاج المزيد من ال
+
+175
+00:13:41,930 --> 00:13:45,930
+RBCs فبالتالي نسبة ال homoglobin هتكون عالية أيضا
+
+176
+00:13:45,930 --> 00:13:50,410
+ال gender أو ال sex of person، نوع الشخص male أو
+
+177
+00:13:50,410 --> 00:13:54,290
+female حكينا إن ال male عندهم نسبة RBCs ونسبة
+
+178
+00:13:54,290 --> 00:13:58,890
+homoglobin أعلى، ليش؟ لإن أول إشي عندهم كتلة عضلية
+
+179
+00:13:58,890 --> 00:14:06,400
+أكثرعندهم muscle mass أعلى تمام و أيضا انه ال
+
+180
+00:14:06,400 --> 00:14:10,480
+female عندها regular blood loss تمام regular blood
+
+181
+00:14:10,480 --> 00:14:16,160
+loss normal values in an adult بتراوح ما بين اتناش
+
+182
+00:14:16,160 --> 00:14:19,980
+لجرام و بصراحة بدقة أكتر خلينا نستعرضها لحتى نعرف
+
+183
+00:14:19,980 --> 00:14:22,660
+ال normal ranges نستعرض هذا الجدول
+
+184
+00:14:25,920 --> 00:14:30,340
+أطفال حديثي الولادة وعمر أسابيع طبعا بيكون عندهم
+
+185
+00:14:30,340 --> 00:14:34,040
+نسبة ال homoglobin عالية بيبدأ يكبر شوية، بيبدأ
+
+186
+00:14:34,040 --> 00:14:38,260
+يصير .. يصير عنده مستوى أو هو المستوى ال adult من
+
+187
+00:14:38,260 --> 00:14:41,080
+ال homoglobin أو ال adult range of homoglobin يعني
+
+188
+00:14:41,080 --> 00:14:45,680
+هو ال ..ال range المتعارف عليه انه يعني ال adult
+
+189
+00:14:45,680 --> 00:14:50,640
+men من 14 ل 18 gram per deciliter و ال woman من 12
+
+190
+00:14:50,640 --> 00:14:54,240
+ل 16 gram per deciliter عندنا ال woman after
+
+191
+00:14:54,240 --> 00:14:58,000
+middle age بعد منتصف عمرها مع ال blood loss or
+
+192
+00:14:58,000 --> 00:15:02,400
+dehumidification و الحمل و الولادة و ال
+
+193
+00:15:02,400 --> 00:15:05,840
+physiological need تبعتهافبصير عندها نسبة ال
+
+194
+00:15:05,840 --> 00:15:12,300
+homoglobin من 11.7 ل 13.8 أو من 11 ل 14 gram per
+
+195
+00:15:12,300 --> 00:15:17,840
+deciliter above normal homoglobin levels is called
+
+196
+00:15:17,840 --> 00:15:22,120
+polycythemia عندي homoglobin أعلى من الطبيعي بسمي
+
+197
+00:15:22,120 --> 00:15:26,060
+هذه الحالة polycythemia و below normal homoglobin
+
+198
+00:15:26,060 --> 00:15:30,140
+level homoglobin أقل من الطبيعي بسمي هذه الحالة
+
+199
+00:15:30,140 --> 00:15:36,460
+anemiaوهذا الاشي بيذكرنا ال hematocrite لما ذكرنا
+
+200
+00:15:36,460 --> 00:15:39,240
+ال hematocrite أو شرحنا ال hematocrite حكينا إنه
+
+201
+00:15:39,240 --> 00:15:42,600
+زيادة hematocrite above the normal أعلى من ال
+
+202
+00:15:42,600 --> 00:15:46,480
+normal بسميها polycythemia و hematocrite below
+
+203
+00:15:46,480 --> 00:15:50,670
+normal أقل من ال normal بسميه anemiaهلأ إيش يعني
+
+204
+00:15:50,670 --> 00:15:55,570
+polycythemia؟ هي حالة مرضية إما نتيجة cancer أو
+
+205
+00:15:55,570 --> 00:16:01,750
+organ disorder تمام؟ وبينتج عنها زيادة في تصنيع ال
+
+206
+00:16:01,750 --> 00:16:08,160
+RBCs يعني زيادة في ال homoglobin levelوتقسم إلى
+
+207
+00:16:08,160 --> 00:16:12,160
+نوعين primary polycythemia و secondary
+
+208
+00:16:12,160 --> 00:16:15,900
+polycythemia خلينا نبدأ في ال primary polycythemia
+
+209
+00:16:15,900 --> 00:16:20,780
+ال primary يا بنات يعني الخلل في مصنع ال herpes
+
+210
+00:16:20,780 --> 00:16:25,650
+يعني في ال bone marrowهناك خلايا أم لل RBCs صار
+
+211
+00:16:25,650 --> 00:16:29,950
+فيها طفرة اكتسبت سلوك in malignant صارت cancer
+
+212
+00:16:29,950 --> 00:16:35,050
+cell صارت تتكثر بشكل مجنون تنتجلي RBCs كتير هاي ال
+
+213
+00:16:35,050 --> 00:16:38,250
+RBCs تطلع ع ال circulation في ال circulation عندي
+
+214
+00:16:38,250 --> 00:16:42,270
+عدد ب RBCs عالي إذا نسبتهم مجلوبينة عالية بسميها
+
+215
+00:16:42,270 --> 00:16:46,230
+primary polycythemia هاي الحالة أما ال secondary
+
+216
+00:16:46,230 --> 00:16:51,140
+polycythemiaقولنا organ disorder، مشكلة في عضو
+
+217
+00:16:51,140 --> 00:16:57,440
+معين، في نسيج معين، تمام؟ أو ظروف معينة، تمام؟ طيب
+
+218
+00:16:57,440 --> 00:17:01,640
+زي مثلا حكينا احنا ذكرنا ال severe lung و ال heart
+
+219
+00:17:01,640 --> 00:17:05,520
+disease، مشكلة في ال lungفاش تنفس كفاية، فاش
+
+220
+00:17:05,520 --> 00:17:09,940
+أكسوجين كفاية بيفوت على الجسم أو مشكلة في القلب،
+
+221
+00:17:09,940 --> 00:17:13,840
+فاش ضخ كافي للدم، يعني فاش توصيل أكسوجين كافي
+
+222
+00:17:13,840 --> 00:17:17,340
+للدم، هذا إيش راح يعمل؟ راح يخلي ال bone marrow
+
+223
+00:17:17,340 --> 00:17:21,700
+اللي بيشتغل و هو مغم عينيه تمام، بيشتغل على عميان
+
+224
+00:17:22,110 --> 00:17:25,530
+بدون ما يعرفون المشكلة بس بيحس ان نسبة ال oxygen
+
+225
+00:17:25,530 --> 00:17:29,610
+قلت بيبدأ يصنع، اذا هذه المشكلة هتخلى ال bone
+
+226
+00:17:29,610 --> 00:17:35,570
+marrow يصنع لي RBCs بزيادة نتيجة الhypoxia، نتيجة
+
+227
+00:17:35,570 --> 00:17:38,710
+نقص ال oxygen اللي حاصل في ال tissue فبالتالي
+
+228
+00:17:38,710 --> 00:17:42,790
+زيادة ال RBCs هذه اللي نتجات راح ينتج عنها زيادة
+
+229
+00:17:42,790 --> 00:17:47,120
+في ال homoglobin levelأيضا ال dehydration،
+
+230
+00:17:47,120 --> 00:17:52,720
+dehydration يعني جفاف، فقدان للماء أو لسوائل الجسم
+
+231
+00:17:52,720 --> 00:17:59,280
+نتيجة حروق أو دياريا أو vomiting، تمام؟ أي سبب
+
+232
+00:17:59,280 --> 00:18:05,740
+بيفقد .. بيفقد الجسم سوائل إذاهذا رح يخلّي الجسم
+
+233
+00:18:05,740 --> 00:18:11,740
+.. محتويات الدم تتركز إذا زاد تركيز ال RBCs نتيجة
+
+234
+00:18:11,740 --> 00:18:16,200
+فقدان السوائل هذه فقدان ال plasma اللي موجودة في
+
+235
+00:18:16,200 --> 00:18:20,660
+ال blood إذا نسبة ال RBCs هتكون أعلى إذا نسبة ال
+
+236
+00:18:20,660 --> 00:18:24,280
+homoglobin هتكون عالية living at high altitude
+
+237
+00:18:24,280 --> 00:18:29,460
+حكينا الناس اللي عايشة على مرتفعاتعالية عندهم high
+
+238
+00:18:29,460 --> 00:18:35,620
+boxia تمام و بالتالي هيتم تصنيع RBC's زيادة عندهم
+
+239
+00:18:35,620 --> 00:18:41,060
+و ..أيضًا هيكون فيه عندى نسبة الـ Homoglobin عالية
+
+240
+00:18:41,060 --> 00:18:46,940
+«هيلي سموكنج» الناس اللى عندهم يعني بدخنه كتير
+
+241
+00:18:46,940 --> 00:18:49,940
+بدخن يعني هو بيدخل .. بي .. بي .. في عنده نقص في
+
+242
+00:18:49,940 --> 00:18:52,980
+الـ Oxygen بدخل قتران و ثانو أكسيد الكربون و أول
+
+243
+00:18:52,980 --> 00:18:56,600
+أكسيد الكربون إلى جسمه بدلًا من الأكسوجين هذا
+
+244
+00:18:56,600 --> 00:18:59,520
+أيضًا برضه كمان الـ bone marrow راح يبدأ يصنعلي
+
+245
+00:18:59,520 --> 00:19:02,980
+RBC's زيادة عشان يعوض الـ Hypoxia فبالتالي بيزيد
+
+246
+00:19:02,980 --> 00:19:07,280
+الـ Homoglobinتمام؟ بالإضافة إلى إنه عندهم في
+
+247
+00:19:07,280 --> 00:19:10,900
+الناس هدول عندهم نسبة homoglobin مؤكسد وإحنا لما
+
+248
+00:19:10,900 --> 00:19:16,680
+بنقيس نسبة ال homoglobin في الفحص لازم نأكسده يعني
+
+249
+00:19:16,680 --> 00:19:21,080
+و بعدين نقيسه فإذا هو عندهم already مؤكسد و جاهز
+
+250
+00:19:21,080 --> 00:19:23,960
+فهذا برضه كمان هيرفعلي نسبة ال homoglobin عندهم في
+
+251
+00:19:23,960 --> 00:19:31,760
+الفحص بس بدي أكتبلكم كلمة hypoxia hypo يعني قليل
+
+252
+00:19:31,760 --> 00:19:43,870
+oxiaيعني نقص oxygen في الجسم تمام؟ low oxygen
+
+253
+00:19:43,870 --> 00:19:50,750
+level low oxygen level تمام
+
+254
+00:19:59,680 --> 00:20:04,500
+طيب blue normal homoglobin level أقل من الطبيعي
+
+255
+00:20:04,500 --> 00:20:09,560
+مستوى ال homoglobin هنسمي هاي الحالة anemia و هاي
+
+256
+00:20:09,560 --> 00:20:14,040
+الحالة تظهر في ال iron deficiency anemia بال
+
+257
+00:20:14,040 --> 00:20:18,560
+deficiencies في ال essential vitamins أو elements
+
+258
+00:20:18,560 --> 00:20:25,660
+زي يكون في عندى deficiency في vitamin B12 في
+
+259
+00:20:25,660 --> 00:20:31,310
+الفولاد و ال bisexطيب ال iron و ال B12 و ال folate
+
+260
+00:20:31,310 --> 00:20:35,890
+و ال B6 هذي عبارة عن molecules مهمة جدا لتصنيع ال
+
+261
+00:20:35,890 --> 00:20:42,710
+homoglobin عندي نقص في ال homoglobinبسبب انه صار
+
+262
+00:20:42,710 --> 00:20:45,950
+في عندي نقص في الحديد طبعا صار عندي نقص حديد او
+
+263
+00:20:45,950 --> 00:20:50,770
+نقص في فيتامين ال B12 في الفولات في ال B6 هذه
+
+264
+00:20:50,770 --> 00:20:56,610
+عبارة عن عناصر مهمة جدا لتصنيع ال homoglobin و
+
+265
+00:20:56,610 --> 00:21:02,030
+لنضوج ال RBCs في ال bone marrowخلل فيهم أو نقصان
+
+266
+00:21:02,030 --> 00:21:07,790
+فيهم مش هيتصنع homoglobin كافي أو مش هتنضج ال RBCs
+
+267
+00:21:07,790 --> 00:21:13,010
+بالوقت الكافي لإلها الحاصل نقص في ال RBCs و ال
+
+268
+00:21:13,010 --> 00:21:15,950
+RBCs أيضا هيكون محتوها فاضي من ال homoglobin
+
+269
+00:21:16,660 --> 00:21:21,140
+inherited homoglobin defect لما يكون المشكلة عندي
+
+270
+00:21:21,140 --> 00:21:25,960
+مشكلة mutation حدثت في الجين المسؤول عن تصنيع ال
+
+271
+00:21:25,960 --> 00:21:30,840
+globin chain ال alpha و ال beta chain معناها صار
+
+272
+00:21:30,840 --> 00:21:35,740
+في عندي مشكلة في ترتيب ال homoglobinهيأثر أيضًا
+
+273
+00:21:35,740 --> 00:21:40,760
+على وظيفة هذا ال homoglobin وعلى وجوده طبعًا خلل
+
+274
+00:21:40,760 --> 00:21:44,500
+في ال homoglobin إذا خلل في وظيفة ال RBCs إذا ال
+
+275
+00:21:44,500 --> 00:21:49,860
+RBCs بتروح ash الجسم بيبدأ يتخلص منها يكسرها إذا
+
+276
+00:21:49,860 --> 00:21:56,000
+المحصلة فقدان RBCs أو تكسير RBCs يعني RBCs قليلة
+
+277
+00:21:56,000 --> 00:22:00,320
+يعني homoglobin level قليلوها دي بنشوفها في حالات
+
+278
+00:22:00,320 --> 00:22:03,820
+ال sickles الانيميا، انيميا الخلايا المنجلية
+
+279
+00:22:03,820 --> 00:22:09,040
+والثلاثيميا، انيميا البحر الأبيض أو حوض البحر
+
+280
+00:22:09,040 --> 00:22:13,830
+الأبيض المتوسطother inherited defect affecting
+
+281
+00:22:13,830 --> 00:22:18,210
+their blood cell، أي مشكلة وراثية تؤثر على ال
+
+282
+00:22:18,210 --> 00:22:21,530
+RBCs، على شكلها، على تركيبها، على وظيفتها، على ال
+
+283
+00:22:21,530 --> 00:22:26,830
+structure تبعها وعلى عمرها، متوسط عمرها، أيضا كمان
+
+284
+00:22:26,830 --> 00:22:31,900
+راح يعطيني below normal homoglobin levelالـ
+
+285
+00:22:31,900 --> 00:22:37,100
+excessive bleeding نظيف حد تمام هذا يؤدي إلى فقدان
+
+286
+00:22:37,100 --> 00:22:43,660
+لل RBCs و أيضا بالتالي فقدان .. نقص في ال RBCs و
+
+287
+00:22:43,660 --> 00:22:48,520
+بلو normal homoglobin level طبعا الجسم بيضل بعد ال
+
+288
+00:22:48,520 --> 00:22:51,760
+bleeding فترة طويلة و هي نسبة ال homoglobin عنده
+
+289
+00:22:51,760 --> 00:22:55,040
+قليلة و ال hematocrit عنده قليل أيضا اللي هو يعني
+
+290
+00:22:55,040 --> 00:22:59,980
+نسبة ال RBCs ليش؟ لإن الجسم بيعود السوائل ل plasma
+
+291
+00:23:00,610 --> 00:23:05,370
+بطريقة أسرع مما بيعود خلايا excessive destruction
+
+292
+00:23:05,370 --> 00:23:08,390
+of red blood cell لو أنا فاني عندي مشكلة في تركيب
+
+293
+00:23:08,390 --> 00:23:15,470
+ال RBCs فالجسم فبتكسر بسهولة في الجسم
+
+294
+00:23:15,470 --> 00:23:21,400
+في ال circulation تمام؟ أو حتى في ال ..الـ spleen
+
+295
+00:23:21,400 --> 00:23:28,400
+في التخال تمام؟ فهذا راح يأدي إلى أن ال RBC's قصر
+
+296
+00:23:28,400 --> 00:23:35,100
+عمرها، اتكسرت بسرعة، نقص في ال RBC's إذا نقص في ال
+
+297
+00:23:35,100 --> 00:23:38,780
+homoglobinkidney disease ايش يعني ايش علاقة ال
+
+298
+00:23:38,780 --> 00:23:42,560
+kidney disease في ال blood؟ شوفوا معايا من ال
+
+299
+00:23:42,560 --> 00:23:46,140
+kidney بينتج هرمون اسمه erythropoietin او
+
+300
+00:23:46,140 --> 00:23:50,800
+erythropoietin تمام؟ هذا الهرمون ال erythropoietin
+
+301
+00:23:50,800 --> 00:23:57,220
+هو اللي بيروح على ال bone مرةو بيكون حامل signal،
+
+302
+00:23:57,220 --> 00:24:01,020
+اشهد ال signal، إشارة، بيقوله في في ال circulation
+
+303
+00:24:01,020 --> 00:24:06,860
+hypoxia صنع RBC's زيادة عشان تحمل ال oxygen وتوديه
+
+304
+00:24:06,860 --> 00:24:15,720
+للخلاياتمام؟ إذا الاريثروبيتين هو ال signal لتصنيع
+
+305
+00:24:15,720 --> 00:24:21,180
+ال RBCs عندي في ال kidney خلل، إذا مش هيتصنع ال
+
+306
+00:24:21,180 --> 00:24:24,720
+hormone هذا الاريثروبيتين فبالتالي مش هتروح signal
+
+307
+00:24:24,720 --> 00:24:28,660
+لل bone marrow تحكيله صنع RBCs كفاية فبالتالي
+
+308
+00:24:28,660 --> 00:24:32,300
+هيصير في عندي انقص في ال RBCs و below normal
+
+309
+00:24:32,300 --> 00:24:35,740
+homoglobin level أقل من الطبيعي مستوى ال
+
+310
+00:24:35,740 --> 00:24:39,830
+homoglobinboon marrow failure or aplastic anemia
+
+311
+00:24:39,830 --> 00:24:43,850
+فالأفشل في ال boon marrow أو أنه لما ال boon
+
+312
+00:24:43,850 --> 00:24:46,710
+marrow يصير في عنده تليف في حالة ال aplastic
+
+313
+00:24:46,710 --> 00:24:52,090
+anemia ممكن أيضا يصير عندى النقص في عدد ال RBCs
+
+314
+00:24:52,090 --> 00:24:56,810
+فبالتالي blue normal homoglobin level cancers that
+
+315
+00:24:56,810 --> 00:25:01,610
+affect the boon marrow في عندى مشاكل ممكن يتأثر
+
+316
+00:25:01,610 --> 00:25:08,450
+على ال boon marrowبرضه كمان ممكن انها تسببلي بـ
+
+317
+00:25:08,450 --> 00:25:11,050
+low normal homoglobin level كيف؟ احنا من شوية
+
+318
+00:25:11,050 --> 00:25:14,350
+حكينا cancer بيعطيني polycythemia فبتزيد ال RBC's
+
+319
+00:25:14,350 --> 00:25:18,430
+فبزيد ال homoglobin لأ شوفوا معايا، اذا كان ال
+
+320
+00:25:18,430 --> 00:25:23,130
+cancer في الخلايا الأم لال RBC's، اذا المحصلة
+
+321
+00:25:23,130 --> 00:25:27,850
+polycythemia زيادة RBC's زيادة homoglobin، primary
+
+322
+00:25:27,850 --> 00:25:33,280
+polycythemiaلكن إذا كان ال cancer في الخلايا الأم
+
+323
+00:25:33,280 --> 00:25:38,020
+لل white bc أو ل البليتلت فهذه خلايا صارت cancer
+
+324
+00:25:38,020 --> 00:25:41,960
+بدا تتكاثر كتير و بدا تكتسح مساحة كتير كبيرة من
+
+325
+00:25:41,960 --> 00:25:45,660
+البون مارو على حساب مين؟ على حساب الخلايا الأم لل
+
+326
+00:25:45,660 --> 00:25:49,540
+Rbc ببطل إلها وساق فبيصير الخلايا الأم لل Rbc
+
+327
+00:25:49,540 --> 00:25:53,260
+عددها قليل في البون مارو نتيجة وجود خلايا cancer
+
+328
+00:25:53,260 --> 00:25:59,170
+من نقل لل white bcتمام؟ فبالتالي بيصير تصنيع ال
+
+329
+00:25:59,170 --> 00:26:04,390
+RBCs قليل و .. و ال homoglobin level قليل أيضا،
+
+330
+00:26:04,390 --> 00:26:07,530
+اذا لما بيكون في عندي حالات لوكيميا اللي هو cancer
+
+331
+00:26:07,530 --> 00:26:11,370
+ال white disease بيكون في .. في المقابل أنيميا،
+
+332
+00:26:11,370 --> 00:26:16,670
+تمام؟measurement of homoglobin احنا كيف بنقيس
+
+333
+00:26:16,670 --> 00:26:21,390
+مستوى ال homoglobin؟ بنقيس ال homoglobin بـ cyano
+
+334
+00:26:21,390 --> 00:26:25,490
+met homoglobin method طبعا بطريقة ال cyano met
+
+335
+00:26:25,490 --> 00:26:30,070
+homoglobin method كيف بالظبط؟ فكرتها او مبدأ عملها
+
+336
+00:26:30,070 --> 00:26:34,650
+انه ال blood ال holy blood طبعا انا باخد عين الدم
+
+337
+00:26:34,650 --> 00:26:40,860
+كامل للفحص بفصلوشليش؟ لأنه أنا بدي ال RBCs ال RBCs
+
+338
+00:26:40,860 --> 00:26:47,480
+هذه هي اللي بدي أنا أخد .. أخدها و .. أكسرها و يتم
+
+339
+00:26:47,480 --> 00:26:51,860
+حصول على ال homoglobin اللي فيها فبالتالي يتفاعل
+
+340
+00:26:51,860 --> 00:26:54,620
+يعطيلي مادة ملونة يتم قياسها على جهاز ال
+
+341
+00:26:54,620 --> 00:26:58,600
+spectrophotometerهذا ال holy blood is diluted
+
+342
+00:26:58,600 --> 00:27:05,300
+بيتخفف بقاش بمحلول بنسميه Drabkin reagent هذا
+
+343
+00:27:05,300 --> 00:27:09,740
+المحلول مكون من potassium ferricyanide و potassium
+
+344
+00:27:09,740 --> 00:27:15,160
+cyanideطبعا اللي بيصير كالتالي انه هذا المحلول
+
+345
+00:27:15,160 --> 00:27:18,560
+بيكسر ال RBC's بيطلع ال homoglobin منها ال
+
+346
+00:27:18,560 --> 00:27:24,580
+homoglobin هذا بيتأكسد بيصيرله oxidized بفعل مين؟
+
+347
+00:27:24,580 --> 00:27:27,340
+بفعل الشق الأول من ال reagent .. من مكونات ال
+
+348
+00:27:27,340 --> 00:27:30,780
+reagent اللي هو ال potassium ferricyanide طب لما
+
+349
+00:27:30,780 --> 00:27:35,700
+بيتأكسد هذا ال homoglobin اش بيعطيني؟ بيعطيني met
+
+350
+00:27:35,700 --> 00:27:41,260
+homoglobin حاجة اسمها met homoglobin طبعا؟و هذا ال
+
+351
+00:27:41,260 --> 00:27:44,420
+met-homoglobin هيروح يتفاعل مع الشق الثاني من ال
+
+352
+00:27:44,420 --> 00:27:48,360
+reagent اللي هو ال potassium cyanide و يتوين لي
+
+353
+00:27:48,360 --> 00:27:53,060
+cyanomet-homoglobin تمام؟ عشان هيك الطريقة اسمها
+
+354
+00:27:53,060 --> 00:27:57,820
+cyanomet-homoglobin method هذا المركب اللي نتج
+
+355
+00:27:57,820 --> 00:28:04,840
+لونه على brown شوية فبالتالي يتم قياسه على 540
+
+356
+00:28:04,840 --> 00:28:11,770
+nanometer تمام؟ على 540 nanometerإذا الـ
+
+357
+00:28:11,770 --> 00:28:16,510
+Homoglobin اللي موجود في ال blood بيكون في iron
+
+358
+00:28:16,510 --> 00:28:20,650
+بالferrous state اللي هو إيش يعني؟ بحالة الferrous
+
+359
+00:28:20,650 --> 00:28:24,530
+اللي هي plus 2 يتأكسد بفعل ال potassium
+
+360
+00:28:24,530 --> 00:28:29,350
+ferricyanide إلى met homoglobin وهذا ال .. ال
+
+361
+00:28:29,350 --> 00:28:32,390
+homoglobin المؤكسد بما يختلف عن ال homoglobin
+
+362
+00:28:32,390 --> 00:28:38,590
+الطبيعي بإنه ال iron تحول من ferrous إلى ferric من
+
+363
+00:28:38,590 --> 00:28:46,070
+plus 2لأ plus تلاتة تمام؟هذا الـ met-homoglobin
+
+364
+00:28:46,070 --> 00:28:50,810
+بفعل الـ potassium cyanide يتحول إلى cyanomet
+
+365
+00:28:50,810 --> 00:28:57,150
+-homoglobin colored substance يتم قراءتها على 540
+
+366
+00:28:57,150 --> 00:29:01,810
+نانومتر وبنقيص طبعاً كمية الـ cyanomet-homoglobin
+
+367
+00:29:01,810 --> 00:29:06,250
+اللي هتعكس لكمية الـ homoglobin في العينة على طول
+
+368
+00:29:06,250 --> 00:29:09,350
+الموج 540 نانومتر باستخدام جهاز الـ
+
+369
+00:29:09,350 --> 00:29:14,650
+spectrophotometerال .. التركيز يتناسب تناسب ترضي
+
+370
+00:29:14,650 --> 00:29:18,710
+مع ال absorbance تمام؟ و هذا الكلام حكيناه و
+
+371
+00:29:18,710 --> 00:29:22,390
+شرحناه في محاضرة ال spectrophotometer حكينا إنه
+
+372
+00:29:22,390 --> 00:29:26,510
+أنا اللي بختار الطول الموجي اللي بدياه يفوت على
+
+373
+00:29:26,510 --> 00:29:31,230
+العينة على أي أساس بختاره بحيث إنه المادة المراد
+
+374
+00:29:31,230 --> 00:29:36,730
+قياسها تعطيني أقصى كمية absorbance عند هذا الطول
+
+375
+00:29:36,730 --> 00:29:39,570
+الموجيالمادة اللى نتجت اللى اسمها
+
+376
+00:29:39,570 --> 00:29:43,490
+cyanomethomoglobin اللى هتعكسلى كمية ال homoglobin
+
+377
+00:29:43,490 --> 00:29:48,430
+اللى اتفاعل طبعا لونها حكينا brown على brown
+
+378
+00:29:48,430 --> 00:29:53,330
+شريدلا براون هك لونها طبعا فبالتالي هذه المادة
+
+379
+00:29:53,330 --> 00:29:59,770
+بتعطينى أقصى قيمة امتصاص على طول موجى 540 علشان هك
+
+380
+00:29:59,770 --> 00:30:02,370
+انا اخترت هذا الطول الموجى او الشركات المصنعة على
+
+381
+00:30:02,370 --> 00:30:05,950
+الفعس هذا او الطريقة هاى بتنصحنى ان انا استخدم هذا
+
+382
+00:30:05,950 --> 00:30:10,800
+الطول الموجى للقياسself-hemoglobin is not
+
+383
+00:30:10,800 --> 00:30:16,040
+converted by cyanomethamoglobin طبعا في أحيانا
+
+384
+00:30:16,040 --> 00:30:24,520
+الناس بتاخد أدوية في من مكوناتهامجموعة الصلفة
+
+385
+00:30:24,520 --> 00:30:28,060
+فبالتالي هذه الصلفة بتتاحت مع الهموجلبين مكونة
+
+386
+00:30:28,060 --> 00:30:32,200
+مركبة اسمه صلف هموجلبين هذا الصلف هموجلبين يعطي
+
+387
+00:30:32,200 --> 00:30:38,140
+لون يكسب ال blood لون أخضر وأيضا بيكون غير قابل
+
+388
+00:30:38,140 --> 00:30:41,920
+قياسه بطريقة سيانوم الهموجلبين يعني صعب احنا
+
+389
+00:30:41,920 --> 00:30:44,980
+نأكسده وبعدين ل مت هموجلبين ونحوله لسيانوم
+
+390
+00:30:44,980 --> 00:30:48,800
+الهموجلبين عشان نقياسه إذا لازم نستخدم طريقة أخرى
+
+391
+00:30:48,800 --> 00:30:54,940
+لقياسهطبعاً الطريقة احنا لو .. لو بتتذكروا بفحص
+
+392
+00:30:54,940 --> 00:31:00,540
+السكر جيبنا tube standard وضفت عليها reagent و
+
+393
+00:31:00,540 --> 00:31:05,460
+tube العينة وضفت عليها reagent اعملتلهم تفاعل مع
+
+394
+00:31:05,460 --> 00:31:10,280
+بعض incubation لفترة معينة ظهر لونسفرت على ال
+
+395
+00:31:10,280 --> 00:31:15,440
+blank اللي هو ال reagent لحاله عشان ألغي اللون
+
+396
+00:31:15,440 --> 00:31:18,320
+اللي جاي من ال reagent و بقيس ال absorbance لل
+
+397
+00:31:18,320 --> 00:31:21,780
+standard و ال absorbance لل test و بروح أطبق في
+
+398
+00:31:21,780 --> 00:31:27,260
+Beer Lambert لو هذا بالنسبة لفحص السكر لكن بالنسبة
+
+399
+00:31:27,260 --> 00:31:31,980
+لفحص ال homoglobin الأمر مختلف نوعا مابدل ما انا
+
+400
+00:31:31,980 --> 00:31:35,740
+اجيب tube واحدة standard بجيب قنابيب من ال
+
+401
+00:31:35,740 --> 00:31:39,620
+standard طبعا بعمل عشان بدي اعمل حاجة اسمها
+
+402
+00:31:39,620 --> 00:31:43,680
+standard curve preparation هلأ ال standard بيجيني
+
+403
+00:31:43,680 --> 00:31:49,080
+مركز من الشركة بتركيز 25 gram per deciliter جاهز
+
+404
+00:31:49,080 --> 00:31:52,660
+محلول homoglobin معلوم التركيز لإن أنا بفحص
+
+405
+00:31:52,660 --> 00:31:56,140
+homoglobin في العينة فلازم المحلول يكون homoglobin
+
+406
+00:31:56,340 --> 00:31:59,220
+لأن في النهاية أنا بدي أقيس اللون اللي نتج من
+
+407
+00:31:59,220 --> 00:32:04,420
+المادة cyanomet-homoglobin فلازم ال standard أيضًا
+
+408
+00:32:04,420 --> 00:32:07,640
+يكون فيه homoglobin عشان يتفاعل مع ال reagent
+
+409
+00:32:07,640 --> 00:32:10,940
+ويعطيهم ال cyanomet-homoglobin اللي أيضًا تقاسم
+
+410
+00:32:10,940 --> 00:32:17,860
+على ال 540 nanometer زيها زي العينةتمام؟ اللهم إنه
+
+411
+00:32:17,860 --> 00:32:22,440
+في اختلاف بسيط هنا إنه بدل إطباء طيوب واحدة من ال
+
+412
+00:32:22,440 --> 00:32:26,700
+standard أنا بحضر عدة قنابيب هذه القنابيب فيها
+
+413
+00:32:26,700 --> 00:32:31,960
+طبعا تركيز متدرجة من ال standard من ال homoglobin
+
+414
+00:32:31,960 --> 00:32:35,440
+طبعا هو ال standard محلول homoglobin إذا هتكون فيه
+
+415
+00:32:35,440 --> 00:32:39,180
+تركيز متدرجة من ال homoglobin في هذه التخفيفات من
+
+416
+00:32:39,180 --> 00:32:45,400
+ال standardبجيب 6 قناة بيبليك يوم الأولى هحط فيها
+
+417
+00:32:45,400 --> 00:32:49,720
+zero من ال standard و خمسة أمقال من ال Drapkin
+
+418
+00:32:49,720 --> 00:32:52,880
+reagent ليه Drapkin reagent؟ هو عبارة عن إيش؟ هو
+
+419
+00:32:52,880 --> 00:32:57,940
+عبارة عن ال potassium ferricyanide و ال potassium
+
+420
+00:32:57,940 --> 00:33:02,230
+cyanide هدول حكينا إيش اسمهم؟Drapkin reagent إذا
+
+421
+00:33:02,230 --> 00:33:05,790
+ال reagent اللي يستخدم في فحص ال homoglobin اسمه
+
+422
+00:33:05,790 --> 00:33:09,590
+Drapkin reagent ومهم جدا تحفظوها الكلمة لإنها
+
+423
+00:33:09,590 --> 00:33:12,570
+بتضالها مستمرة معاكم كل ما بدك تشتغلي homoglobin
+
+424
+00:33:12,570 --> 00:33:17,050
+أو تشتغلي CBC أو كذا لازم ينذكر ل Drapkin reagent
+
+425
+00:33:17,050 --> 00:33:23,710
+تمام؟طيب، في هذه الحالة أنا بدي أصفر عليها،
+
+426
+00:33:23,710 --> 00:33:26,410
+عالجهاز، لإنه مافيش فيها غير dropkin reagent، بدي
+
+427
+00:33:26,410 --> 00:33:29,930
+ألغي اللون اللي جاي من ال reagent هذا ال dropkin
+
+428
+00:33:29,930 --> 00:33:33,630
+reagent يا بنات لونه على أصفر، تمام؟ و هذا اللون
+
+429
+00:33:33,630 --> 00:33:36,950
+الأصفر ممكن يعطي أثر على النتائج لإن هو عبارة عن
+
+430
+00:33:36,950 --> 00:33:41,390
+محلول ملون لحاله، من دون تفاعل هو محلول ملون، فأنا
+
+431
+00:33:41,390 --> 00:33:45,360
+لازم ألغي اللون اللي جاي منه، تمام؟فعشان هيك أنا
+
+432
+00:33:45,360 --> 00:33:48,860
+بصفر عليها و لإنه أيضا تركيزي ال homoglobin فيه
+
+433
+00:33:48,860 --> 00:33:52,700
+صفر أنا ماحطيت standard فهو هذا ال zero تبعي هو ال
+
+434
+00:33:52,700 --> 00:33:55,580
+.. ال .. ال blank تبعي اللي أنا لازم أصفر عليه
+
+435
+00:33:55,580 --> 00:33:58,360
+الاشي .. ال tube الفاضية من العينة أو من المادة
+
+436
+00:33:58,360 --> 00:34:03,630
+المراد قياسها بسميها blankطيب ال tube رقم 2 إيش
+
+437
+00:34:03,630 --> 00:34:07,230
+حطيت فيها؟ حطيت فيها one Amol من ال standard و 4
+
+438
+00:34:07,230 --> 00:34:10,930
+Amol من ال Drabkin طيب خلينا نشوف طبعا من وين أنا
+
+439
+00:34:10,930 --> 00:34:15,110
+أخدت ال standard هذا؟ أخدته من ال stock اللي عندي
+
+440
+00:34:15,110 --> 00:34:18,770
+اللي تركيزه 25 gram per deciliter طيب أنا كيف ممكن
+
+441
+00:34:18,770 --> 00:34:24,330
+أحسب تركيز ال tube رقم 2؟ على طول بطبق قانون ال mv
+
+442
+00:34:24,330 --> 00:34:29,220
+يساوي mvال .. ال tube او ال .. ال stock عندي كان
+
+443
+00:34:29,220 --> 00:34:34,740
+تركيزه 25، كام أخدت منه؟ أخدت منه one ml، كام أصبح
+
+444
+00:34:34,740 --> 00:34:38,020
+التركيز؟ هذا اللي بدي أعرفه، طب التركيز .. ال ..
+
+445
+00:34:38,020 --> 00:34:40,960
+ال volume النهائي، والله هاي one ml، حطيت عليها
+
+446
+00:34:40,960 --> 00:34:44,280
+أربع ml من ال drop كان صاروا خمسة، فهي اذا هنا
+
+447
+00:34:44,280 --> 00:34:50,800
+خمسة عندي، اذا من .. بهذه الطريقة بحسبالتركيز اللي
+
+448
+00:34:50,800 --> 00:34:55,860
+هو عبارة عن خمسة جرام per deciliter طبعا و بقيس ال
+
+449
+00:34:55,860 --> 00:34:58,660
+absorbance على جهاز ال spectrophotometer هاي هانا
+
+450
+00:34:58,660 --> 00:35:05,280
+ميعطيني قراءات افتراضية طيب ال youtube رقم تلاتة
+
+451
+00:35:05,280 --> 00:35:11,260
+اتنين و نصو من ال standard و اتنين و نص من ال led
+
+452
+00:35:11,260 --> 00:35:14,240
+rapkin اصبح ممكن انا برضه كمان احسب لها تركيزها
+
+453
+00:35:14,240 --> 00:35:19,480
+بحيث انه ال .. ال .. ال tube رقم تلاتة sorry ال
+
+454
+00:35:19,480 --> 00:35:23,800
+tube رقم تلاتة انا جبتلها خمسة و عشرين طبعا اخدت
+
+455
+00:35:23,800 --> 00:35:27,400
+من ال stock اللي هو تركيزه خمسة و عشرين اخدت اتنين
+
+456
+00:35:27,400 --> 00:35:29,960
+و نص تمام؟ و ..
+
+457
+00:35:34,300 --> 00:35:39,000
+كم هيطلع التركيز هو اللي احنا بدناياه في ال final
+
+458
+00:35:39,000 --> 00:35:43,860
+volume عندى اتنين و نص من ال standard و اتنين و نص
+
+459
+00:35:43,860 --> 00:35:50,140
+من ال drafting يعني خمسة و بجيب التركيزبهذا الشكل
+
+460
+00:35:50,140 --> 00:35:55,240
+لباقي القنابيب لنبوبة رقم أربعة بحط فيها اتنين
+
+461
+00:35:55,240 --> 00:36:00,940
+أمقال من ال standard وتيوب رقم .. و بحط فيها تلاتة
+
+462
+00:36:00,940 --> 00:36:07,660
+أمقال من ال drapkin لتيوب رقم خمسة بحط فيها تلاتة
+
+463
+00:36:07,660 --> 00:36:10,440
+أمقال من ال standard و اتنين من ال drapkin و
+
+464
+00:36:10,440 --> 00:36:13,540
+الأتيوب الأخيرة أربعة أمقال من ال standard و one
+
+465
+00:36:13,540 --> 00:36:19,360
+أمقال من ال drapkinتمام؟ بهذا الشكل بحسب التركيز
+
+466
+00:36:19,360 --> 00:36:23,080
+بقانون MV يساوي MPV دربوا إيداكم عليها واحفظوا او
+
+467
+00:36:23,080 --> 00:36:27,560
+احسبوا التركيز لإلها طبعا كل تيوب يا بنات بروح إيش
+
+468
+00:36:27,560 --> 00:36:31,680
+بجيب؟ بجيب ال absorbance لإلها على جهاز ال
+
+469
+00:36:31,680 --> 00:36:36,340
+spectrophotometer بعد ما أصفر على مين؟ أصفر على ال
+
+470
+00:36:36,340 --> 00:36:41,010
+blank على ال zero أصفر على ال reagentو انا انا انا
+
+471
+00:36:41,010 --> 00:36:42,070
+انا انا انا انا انا انا انا انا انا انا انا انا
+
+472
+00:36:42,070 --> 00:36:42,290
+انا انا انا انا انا انا انا انا انا انا انا انا
+
+473
+00:36:42,290 --> 00:36:51,370
+انا انا انا انا انا انا انا
+
+474
+00:36:54,700 --> 00:36:58,980
+فبحصل على عدة قراءات من ال absorbance و عدة قراءات
+
+475
+00:36:58,980 --> 00:37:03,060
+من ال concentration هدول أنا بروح إيش بعمل؟ بطبق
+
+476
+00:37:03,060 --> 00:37:07,120
+فيه .. برسم curve طبعا اللي أنا بيبيا بنات بعد ما
+
+477
+00:37:07,120 --> 00:37:10,180
+أنا أخلط ال standard و أخلط ال .. ال .. ال
+
+478
+00:37:10,180 --> 00:37:13,780
+drafting مع بعضهم البعض أعمل mixing لازم أستنى
+
+479
+00:37:13,780 --> 00:37:18,580
+عليهم عشر دقائق قبل لأقرأ ال absorbance بعدين بطلع
+
+480
+00:37:18,580 --> 00:37:22,840
+قراءة ال absorbance و بروح ب .. بعمل .. برسم curve
+
+481
+00:37:23,040 --> 00:37:28,560
+الـ curve هذا بحط ال .. ال absorbance على ال y
+
+482
+00:37:28,560 --> 00:37:32,440
+-axis و ال concentration على ال x-axis بهذا الشكل،
+
+483
+00:37:32,440 --> 00:37:36,660
+هاي ال absorbance و هنا ال concentration فالمفروض
+
+484
+00:37:36,660 --> 00:37:41,540
+إنه العلاقة تطلع معايا طردية، linearليش؟ لأنه
+
+485
+00:37:41,540 --> 00:37:43,840
+حكينا احنا في لما شرحنا محاضرة ال
+
+486
+00:37:43,840 --> 00:37:47,600
+spectrophotometer حكينا انه ال .. ال .. ال
+
+487
+00:37:47,600 --> 00:37:50,400
+absorbance يتناسب تناسب انترضيا مع ال
+
+488
+00:37:50,400 --> 00:37:53,920
+concentration و انا لو جبت .. عملت plotting لأ
+
+489
+00:37:53,920 --> 00:37:56,320
+سالب لك جيتي اللي هو ال absorbance مع ال
+
+490
+00:37:56,320 --> 00:38:01,440
+concentration تظهر عندي علاقة linear تمام رسمت ال
+
+491
+00:38:01,440 --> 00:38:05,240
+curve و طلع عندي علاقة linear بدي .. اش بدي اعمل؟
+
+492
+00:38:05,240 --> 00:38:09,060
+بدي اروح اجيب ال slope لهاي ال ..
+
+493
+00:38:12,190 --> 00:38:16,510
+السلوب لهذا ال curve السلوب هو عبارة عن ايش يا
+
+494
+00:38:16,510 --> 00:38:23,310
+بنات؟ Delta Y على Delta X فرق
+
+495
+00:38:23,310 --> 00:38:24,810
+الصادات على فرق الصين
+
+496
+00:38:27,840 --> 00:38:31,420
+هلأ من ال .. من ال .. من ال slope هذا بدي أحسب
+
+497
+00:38:31,420 --> 00:38:35,920
+حسابات العينة العينة تبعتي كيف بشتغلها؟ بجيب ال
+
+498
+00:38:35,920 --> 00:38:40,080
+fit you بحط 5 ml من ال Drabkin reagent و بضيف
+
+499
+00:38:40,080 --> 00:38:43,660
+عليهم 20 microliter .. microliter من ال holy blood
+
+500
+00:38:43,660 --> 00:38:48,100
+بعملهم mixing منيح بستن 10 دقائق بقرأ ال
+
+501
+00:38:48,100 --> 00:38:53,660
+absorbance على 540 nanometer و على .. طبعا بكون
+
+502
+00:38:53,660 --> 00:38:57,150
+مسفرة على ال plant اللي ..هو التيوب رقم واحد في
+
+503
+00:38:57,150 --> 00:39:06,070
+التيوبات ال standard curve طيب من ال curve اللي
+
+504
+00:39:06,070 --> 00:39:10,930
+أنا رسمته حكينا إنه أنا بدي أجيب slope و .. اللي
+
+505
+00:39:10,930 --> 00:39:13,830
+هو عبارة عن فرق الصدات على فرق الصينة هاي ال slope
+
+506
+00:39:13,830 --> 00:39:17,290
+عبارة عن delta Y على delta X فرق الصدات على فرق
+
+507
+00:39:17,290 --> 00:39:20,850
+الصينة طيب فرق الصدات هو عبارة عن إيش يا بنات؟ هو
+
+508
+00:39:20,850 --> 00:39:24,770
+فرق عن .. فرق absorbance طب absorbance لمين؟
+
+509
+00:39:26,590 --> 00:39:30,870
+absorbance لمين؟ ال absorbance هاد لل standard
+
+510
+00:39:30,870 --> 00:39:34,650
+لإنه أنا برسم standard curve أو برسم curve جاي من
+
+511
+00:39:34,650 --> 00:39:40,010
+أنابيب ل standard تمام؟ إذا هو عبارة عن فرق ل
+
+512
+00:39:40,010 --> 00:39:45,810
+absorbance ل standardطيب فرق الصينات عبارة عن إيش؟
+
+513
+00:39:45,810 --> 00:39:49,850
+فرق ال concentration طب ال concentration هذا لمين؟
+
+514
+00:39:49,850 --> 00:39:53,890
+لل standard إذا برضه كمان فرق ال concentration لل
+
+515
+00:39:53,890 --> 00:39:57,790
+standard إذا بإمكاني أنسى delta Y على delta X و
+
+516
+00:39:57,790 --> 00:40:00,870
+أحكي إنه ال slope هو عبارة عن ال absorbance لل
+
+517
+00:40:00,870 --> 00:40:03,630
+standard، هو عبارة عن قيمة absorbance أو فرق
+
+518
+00:40:03,630 --> 00:40:04,290
+absorbance
+
+519
+00:40:13,170 --> 00:40:16,950
+طيب لو جبت factor اللي هو عبارة عن مقلوب ال slope
+
+520
+00:40:16,950 --> 00:40:23,570
+هو عبارة عن مقلوب ال slope فأصبح عنديإنه هذا ال
+
+521
+00:40:23,570 --> 00:40:28,570
+factor هو عبارة عن concentration لل standard على
+
+522
+00:40:28,570 --> 00:40:32,670
+absorbance لل standard طيب لو أنا جبت هذا ال
+
+523
+00:40:32,670 --> 00:40:37,190
+factor و ضرب .. ضربته ب .. ال absorbance لل عينة
+
+524
+00:40:37,190 --> 00:40:41,530
+المجهولة فإيش صار عندي؟ تأملوا هذا القانون جيدا،
+
+525
+00:40:41,530 --> 00:40:47,390
+مش هو Beer-Lambert Law؟أصبح احنا يعني صح انا
+
+526
+00:40:47,390 --> 00:40:51,750
+اتطبقت على أكثر من أنبوبة standard ولكن في النهاية
+
+527
+00:40:51,750 --> 00:40:57,610
+انا ما طلعت عن اطار Beer Lambert Cloud absorbance
+
+528
+00:40:57,610 --> 00:41:02,450
+لل X على ال absorbance لل standard ضرب تركيز لل
+
+529
+00:41:02,450 --> 00:41:07,910
+standardهذا ال factor في absorbance لل X يعني أصبح
+
+530
+00:41:07,910 --> 00:41:13,350
+أنا بهذه الطريقة بكون عم بطبق Beer-Lambert law بس
+
+531
+00:41:13,350 --> 00:41:19,510
+بدلالة أكثر من cube standard و ليس cube واحد تمام؟
+
+532
+00:41:19,510 --> 00:41:24,690
+هذه هي الفكرة طيب
+
+533
+00:41:24,690 --> 00:41:30,870
+خلونا بس نقارن ما بين فحص السكر و فحص ال
+
+534
+00:41:30,870 --> 00:41:35,440
+homoglobinفحص السكر كانت العينة plasma لإن ال
+
+535
+00:41:35,440 --> 00:41:39,980
+nutrients لجلوكوز و الأشياء المغذية هاي بتكون
+
+536
+00:41:39,980 --> 00:41:45,420
+موجودة في ال plasma أو ال serum فحص ال homoglobin
+
+537
+00:41:45,420 --> 00:41:48,400
+كانت العينة هو ال blood لإن أنا معنية أحصل على
+
+538
+00:41:48,400 --> 00:41:51,760
+خلايا لإن ال homoglobin موجود داخل ال red blood
+
+539
+00:41:51,760 --> 00:41:57,560
+cells فحص السكر استخدمت standard tube واحدةوماكن
+
+540
+00:41:57,560 --> 00:42:00,780
+في داعي ان هنا ارسم curve وطبقت ال beer lambert
+
+541
+00:42:00,780 --> 00:42:06,560
+law مباشرة لكن في فحص ال homoglobin انا عملت لفة
+
+542
+00:42:06,560 --> 00:42:10,440
+هي اي نعم قدتني الى ان انا في النهاية بكون عم
+
+543
+00:42:10,440 --> 00:42:15,580
+بتطبق نفس beer lambert law بس استخدمت اكثر من tube
+
+544
+00:42:15,580 --> 00:42:21,340
+standardو رسمت curve و إنتجت إنه العلاقة ما بين ال
+
+545
+00:42:21,340 --> 00:42:24,480
+absorbance و ال concentration هي linear اتأكدت
+
+546
+00:42:24,480 --> 00:42:28,800
+بنفسي و جبت ال slope فرق الصوادر على فرق الصينات
+
+547
+00:42:28,800 --> 00:42:32,670
+جبت ال factor اللي هو مقلوب لل slopeضربت هذا ال
+
+548
+00:42:32,670 --> 00:42:37,170
+factor ب ال absorbance لا ال test لعينة المريض و
+
+549
+00:42:37,170 --> 00:42:40,870
+في النهاية تفاجأت أنه أنا كنت عم بطبق قانون بير
+
+550
+00:42:40,870 --> 00:42:47,330
+لمبرتلاو بحذافيره ولكن باستخدام اكثر من tube من ال
+
+551
+00:42:47,330 --> 00:42:54,460
+standard بدلالة اكتر من tube من ال standardال ..
+
+552
+00:42:54,460 --> 00:42:58,860
+يختلف الطول الموجي اللي أنا بقرأ عليه فحص السكر عن
+
+553
+00:42:58,860 --> 00:43:04,620
+فحص ال homoglobin و أيضا تختلف الوحدة السكر وحدتها
+
+554
+00:43:04,620 --> 00:43:08,740
+milligram per deciliter و ال homoglobin وحدته
+
+555
+00:43:08,740 --> 00:43:13,550
+اجرام per deciliterيختلف ال normal range أيضا،
+
+556
+00:43:13,550 --> 00:43:20,430
+السكر يلو مستويات معينة من 70 ل110 و ال homoglobin
+
+557
+00:43:20,430 --> 00:43:24,830
+طبعا حسب العمر و حسب ال gender يختلف ال normal
+
+558
+00:43:24,830 --> 00:43:29,470
+range، تمام؟مهم جدا تربطوا الأمور هاي مع بعض لحتى
+
+559
+00:43:29,470 --> 00:43:32,770
+تكونوا فاهمين إحنا إيش بنشتغل وكيف بنفرق وعلى أي
+
+560
+00:43:32,770 --> 00:43:35,570
+أساس بنختار نوع العينة أو بنختار طول الموجي، كيف
+
+561
+00:43:35,570 --> 00:43:38,290
+بشتغل، إيش .. إيش دور ال standard، إيش دور ال
+
+562
+00:43:38,290 --> 00:43:43,830
+blank في التجربة اللي أنا بشتغلها discussion
+
+563
+00:43:45,170 --> 00:43:48,510
+mechanical sources of error أول ما تسمعوا كلمة
+
+564
+00:43:48,510 --> 00:43:52,150
+mechanical sources of error معناها مصادر ال error
+
+565
+00:43:52,150 --> 00:43:56,850
+الخلل اللي تحدث أثناء شغليه، الشغل اليدوي، الشغل
+
+566
+00:43:56,850 --> 00:44:00,210
+العملي، تمام؟ ممكن يكون في عندي by-putting error
+
+567
+00:44:01,000 --> 00:44:04,040
+طبعا هنشتغل يا بنات محلي ال by betting أنا باخد
+
+568
+00:44:04,040 --> 00:44:07,020
+volume من ال reagent على volume من العينة volume
+
+569
+00:44:07,020 --> 00:44:09,940
+من ال reagent على volume من ال standard فهذه كلها
+
+570
+00:44:09,940 --> 00:44:14,160
+باستخدام ال automatic by bet لازم أكون متمرسة أو
+
+571
+00:44:14,160 --> 00:44:18,380
+يعني مرنة إيدي منيح متدربة على ال by bet use و
+
+572
+00:44:18,380 --> 00:44:23,680
+أيضا بستخدمها بدقة و بشكل كتير منيح و صحيةأيضا
+
+573
+00:44:23,680 --> 00:44:28,080
+لازم انا اكون منتبهة و حاذرة باستخدام المحليل يعني
+
+574
+00:44:28,080 --> 00:44:30,880
+مثلا ال drug reagent اللي هتستخدموه في فحص ال
+
+575
+00:44:30,880 --> 00:44:34,660
+homoglobin هو عبارة عن reagent ده اتملم الصابون يا
+
+576
+00:44:34,660 --> 00:44:38,080
+بنات بما انه ده اتملم الصابون اذا
+
+577
+00:44:40,700 --> 00:44:44,400
+هيكون foam و ال foam هذه ممكن تعيق عملية ال by
+
+578
+00:44:44,400 --> 00:44:48,260
+beating أو تحدث خطأ في ال volume فانا لازم اكون من
+
+579
+00:44:48,260 --> 00:44:51,020
+تبه و انا بفضي في هذا المحلول افضي على الجدار و
+
+580
+00:44:51,020 --> 00:44:54,260
+شوي شوي كمان انا بستخدم خمسة امقال من هذا ال
+
+581
+00:44:54,260 --> 00:44:58,020
+reagent كمية مش بسيطة كمية كبيرة تعادة على ال tube
+
+582
+00:44:58,020 --> 00:45:05,340
+فلازم انا لما افضي هذا ال tube اكون يعني اكون
+
+583
+00:45:05,340 --> 00:45:08,960
+حاضرة انه مايكببرة ليت يوم هعلمكم طبعا كيف انا
+
+584
+00:45:08,960 --> 00:45:13,080
+بفضي و كيف باخد ال volume كيف بعمل by beating في
+
+585
+00:45:13,080 --> 00:45:17,180
+الفحص علشان تشتغلوا خطوة بخطوة واحدة واحدة و ما
+
+586
+00:45:17,180 --> 00:45:21,820
+تجلطوا use of dirty or scratches او scratched
+
+587
+00:45:21,820 --> 00:45:22,520
+cubits
+
+588
+00:45:24,930 --> 00:45:28,730
+استخدام cuvette مش clean وفيها scratches احنا
+
+589
+00:45:28,730 --> 00:45:31,650
+حكينا من شروط ال beer lambert لأو الدقيقة و
+
+590
+00:45:31,650 --> 00:45:35,430
+الصحيحة انه يكون عندي ال .. ال .. ال cuvettes
+
+591
+00:45:35,430 --> 00:45:39,250
+النبوب اللي بحط فيها ال tube و هي عبارة عن تعملي
+
+592
+00:45:39,250 --> 00:45:43,530
+sample او solution holder داخل الجهاز لازم تكون
+
+593
+00:45:43,530 --> 00:45:47,750
+clean مش فيها خالية من الشقوق من الكثور الشطوب
+
+594
+00:45:47,750 --> 00:45:55,400
+وبالتالي ماتأثر على مسار الضوءفإذا كان عندي ال
+
+595
+00:45:55,400 --> 00:46:00,760
+cuvettes مش نظيفة، مساكتها بشكل خاطئ، صار في عليها
+
+596
+00:46:00,760 --> 00:46:06,600
+من ال cotton fiber أو تلوثت، صار مشققة، مكسورة،
+
+597
+00:46:06,600 --> 00:46:10,360
+فيها خدش، هذا راح يأثر على القراءة use of
+
+598
+00:46:10,360 --> 00:46:13,100
+deteriorated reagent
+
+599
+00:46:15,970 --> 00:46:21,210
+reaction تالف تمام؟ reaction تالف فاستخدام
+
+600
+00:46:21,210 --> 00:46:26,250
+reaction تالف بالتالي هيعطيني reaction non-proper
+
+601
+00:46:26,250 --> 00:46:29,030
+reaction أو improper reaction مش ال reaction اللي
+
+602
+00:46:29,030 --> 00:46:34,470
+بديها فبالتالي نتيجة مش صحيحة أو دقيقةأيضًا كمان
+
+603
+00:46:34,470 --> 00:46:38,990
+في عندي أمور ياجب مراعاعتها أثناء إجراء الفحص زي
+
+604
+00:46:38,990 --> 00:46:43,230
+مثلًا أنه المحلول اللي أنا بشتغل عليه أو اللي بدي
+
+605
+00:46:43,230 --> 00:46:47,470
+أقيسه يكون أطائي clear صح هو ملون و أنا بدي أقيس
+
+606
+00:46:47,470 --> 00:46:51,630
+شدة لون على الجهاز بس لازم يكونمافيش فيه Akara
+
+607
+00:46:51,630 --> 00:46:54,970
+مافيش فيه precipitation يعني لو شخص عنده ال white
+
+608
+00:46:54,970 --> 00:46:59,850
+pieces عالية كتير هذه هتأدي إلى أنه المحلول اللي
+
+609
+00:46:59,850 --> 00:47:02,870
+بدي أقيصوها فيه نسبة turbidity ال white pieces
+
+610
+00:47:02,870 --> 00:47:08,760
+بتعملي turbidity زيادتهاتمام زيادة ال white bcs
+
+611
+00:47:08,760 --> 00:47:13,180
+هتعمل turbidity إذا هتأثر على ال absorbance هتأثر
+
+612
+00:47:13,180 --> 00:47:17,360
+على مصال الدوة هيزيد ال absorbance فبالتالي يبطيني
+
+613
+00:47:17,360 --> 00:47:23,870
+نسبته مجلوبين falsely elevatedHomoglobin S و
+
+614
+00:47:23,870 --> 00:47:26,990
+Homoglobin C هدول الأنواع من ال Homoglobin ..
+
+615
+00:47:26,990 --> 00:47:29,910
+Homoglobin S هو ال Homoglobin اللي موجود عند الناس
+
+616
+00:47:29,910 --> 00:47:33,630
+اللي مصابين بال Sickness الانيميا .. بانيميا
+
+617
+00:47:33,630 --> 00:47:37,950
+الخلايا المنجالية و ال Homoglobin C برضه كمان
+
+618
+00:47:37,950 --> 00:47:44,050
+بيكون عند ناس مصابين بتفرة أخرى مشابهة زي .. زي ال
+
+619
+00:47:44,050 --> 00:47:48,010
+Sickness الانيميا المحصلة انه هذا ال Homoglobin
+
+620
+00:47:48,010 --> 00:47:54,040
+لما بيتأكسد بيترسبتمام؟ homoglobin s و homoglobin
+
+621
+00:47:54,040 --> 00:47:59,680
+c طب احنا اصلا مبدأ عملنا بيعتمد
+
+622
+00:47:59,680 --> 00:48:02,840
+على تأكسد ال homoglobin على أكسدة ال homoglobin
+
+623
+00:48:02,840 --> 00:48:06,480
+يتحول من homoglobin إلى met homoglobin فتخيل
+
+624
+00:48:06,480 --> 00:48:09,700
+homoglobin s يتحول ل met homoglobin s او
+
+625
+00:48:09,700 --> 00:48:13,960
+homoglobin c يتحول ل met homoglobin c معناه هيترسب
+
+626
+00:48:14,530 --> 00:48:17,250
+طب إحنا مش حكينا إنه من شروط ال dilution not
+
+627
+00:48:17,250 --> 00:48:21,270
+associate or dissociate مايترسبش مايكونش فيه عكارة
+
+628
+00:48:21,270 --> 00:48:24,770
+مايأثرش أنا بدي أقيس بس اللون اللي جاي من المادة
+
+629
+00:48:24,770 --> 00:48:30,390
+الملونة اللي هي cyanomethamoglobin تمام فعشان هيك
+
+630
+00:48:30,390 --> 00:48:35,650
+هذا عشان ألغي تأثير هذا الراسب عن نتيجة ب .. بعمل
+
+631
+00:48:35,650 --> 00:48:39,730
+dilution للعينة باستخدام distilled water أو saline
+
+632
+00:48:39,730 --> 00:48:45,060
+بنسبة 1 إلى 1 يعني ال dilution هيكون نص تمام؟فإذا
+
+633
+00:48:45,060 --> 00:48:51,160
+يعني باخد حجم من العينة وحجم equal مساوي من ال
+
+634
+00:48:51,160 --> 00:48:54,820
+distilled water يعني بنسبة واحد إلى واحد ال total
+
+635
+00:48:54,820 --> 00:48:58,860
+dilution هيكون واحد لواحد ده ايه؟ زائد واحد يعني
+
+636
+00:48:58,860 --> 00:49:05,860
+واحد لنص واحد لاتنين sorry تمام نص فبالتاليال ..
+
+637
+00:49:05,860 --> 00:49:09,020
+ال .. النتيجة تبعتي لما بتطلع أكيد بدي أضربها بال
+
+638
+00:49:09,020 --> 00:49:12,000
+dilution factor اللي هو 2 عشان أحصل على ال
+
+639
+00:49:12,000 --> 00:49:15,820
+original concentration راجعوا ال dilution لأن دوها
+
+640
+00:49:15,820 --> 00:49:20,760
+يكونوا لاحظين إنه كل مرة في كل محاضرة أو كل ما
+
+641
+00:49:20,760 --> 00:49:24,500
+بنتقدم شوية ال dilution بيضل مرافقنا، بيضل معنا،
+
+642
+00:49:24,500 --> 00:49:27,760
+فالمنيح إنه تكونوا مراجعينه وحافظينه ومتمكنين منه
+
+643
+00:49:28,430 --> 00:49:33,290
+Libemia إيش يعني ليبياميا؟ ليبي يعني جاي من لبد،
+
+644
+00:49:33,290 --> 00:49:37,570
+دهون، ايميا حاكينا في الدم، إذا زيادة الدهون في
+
+645
+00:49:37,570 --> 00:49:41,990
+الدم بسميها ليبياميا طبعا الدهون في الدم، الدهون
+
+646
+00:49:41,990 --> 00:49:47,730
+تحت عكارة أصلا يعني، فالحالة هذه ممكن تعطيني false
+
+647
+00:49:47,730 --> 00:49:52,520
+resultفي وجود نسبة .. نسبة عالية من الدهون في الدم
+
+648
+00:49:52,520 --> 00:49:58,020
+فبالتالي يفضل إن أنا الـ Drapkin reagent الـ Blank
+
+649
+00:49:58,020 --> 00:50:03,630
+عينة الـ Blank تبعتيأضيف عليها عشرين microliter
+
+650
+00:50:03,630 --> 00:50:08,570
+اللي هو point zero two ml من البلازمة طب ما هي
+
+651
+00:50:08,570 --> 00:50:11,750
+البلازمة إيش؟ عبارة عن إيش؟ عبارة عن ما موجود فيها
+
+652
+00:50:11,750 --> 00:50:16,250
+الدهون تمام؟ وروح أصفر عليها طب لما أصفر على ال
+
+653
+00:50:16,250 --> 00:50:18,950
+reagent هذا اللي أنا أضفتله عشرين microliter من
+
+654
+00:50:18,950 --> 00:50:23,770
+البلازمة إيش أنا استفدت؟ أنا بكون لغاية اللون اللي
+
+655
+00:50:23,770 --> 00:50:27,270
+جاي من ال reagent و لغاية اللون اللي جاي من
+
+656
+00:50:27,270 --> 00:50:31,590
+البلازمة اللي هو من الدهون اللي في البلازمةلغاية
+
+657
+00:50:31,590 --> 00:50:35,910
+تأثير الدهون اللى موجودة تربدت اللى عاملها الدهون
+
+658
+00:50:35,910 --> 00:50:43,490
+على نتيجة فحصه تمام ال drug in reagent is
+
+659
+00:50:43,490 --> 00:50:47,870
+sensitive to light هذا ال reagent حساس للضوء لازم
+
+660
+00:50:47,870 --> 00:50:51,530
+انه مانعرضه للضوء فبالتالي انا بحطه في ال brown
+
+661
+00:50:51,530 --> 00:50:56,740
+bottle دايما احنا بنخزنههو بيجينا مركز فبنخففه و
+
+662
+00:50:56,740 --> 00:51:01,620
+بحضر منه working solution بخزنه في قنابيب dark
+
+663
+00:51:01,620 --> 00:51:07,580
+لونها brown و بحطها أيضا في أماكن معتمة بعيدة عن
+
+664
+00:51:07,580 --> 00:51:11,540
+الضوء علشان مايتكسر هذا ال reagent مايصيرش
+
+665
+00:51:11,540 --> 00:51:14,940
+deteriorated لإن إذا صار deteriorated يعني في عندي
+
+666
+00:51:14,940 --> 00:51:19,840
+errorطيب carboxy-homoglobin الناس اللي عندها نسبة
+
+667
+00:51:19,840 --> 00:51:23,340
+لها carboxy-homoglobin عالية زي المدخنين فهدول
+
+668
+00:51:23,340 --> 00:51:27,880
+بيحتاج ال .. ال homoglobin هذا ساعة واحدة عشان
+
+669
+00:51:27,880 --> 00:51:32,260
+يتكون ساعة مش عشر دقائق ال incubation period ساعة
+
+670
+00:51:32,260 --> 00:51:36,840
+عشان يتحول لسيانومة homoglobin فهذا طبعا لو انا ..
+
+671
+00:51:36,840 --> 00:51:39,420
+لو ماكنتش عارفة ان الشخص هذا مدخن يعني لأنه عنده
+
+672
+00:51:39,420 --> 00:51:42,840
+carboxy-homoglobin اذا و قرأت خلال عشر دقائق
+
+673
+00:51:45,910 --> 00:51:49,050
+يعني أنا مستهلكتش ساعة كاملة عشان يتحول كل
+
+674
+00:51:49,050 --> 00:51:52,370
+الكربوكسيهوموجلوبين لسيانو متهموجلوبين، إذا هقرأ
+
+675
+00:51:52,370 --> 00:51:56,250
+نتي أنه مش كل الهموجلوبين اتحول فبالتالي ممكن يكون
+
+676
+00:51:56,250 --> 00:52:00,290
+في عندي error بس بحكيلك أنه أحيانا ال degree of
+
+677
+00:52:00,290 --> 00:52:03,350
+error هذه، نسبة ال error هذه بتكونش واضحة كتير،
+
+678
+00:52:03,350 --> 00:52:07,710
+ليش؟ لإنه احنا ذكرنا في بداية المحاضرة أنه ال
+
+679
+00:52:07,710 --> 00:52:12,490
+smokers هدول عندهم نسبة متهموجلوبين عندهم دم أكسد
+
+680
+00:52:12,490 --> 00:52:17,910
+وزاق جاهزتمام؟ فهذا بعادل النسبة، بالعكس أحيانًا
+
+681
+00:52:17,910 --> 00:52:22,030
+بتكون نسبتهم عالية، كمان؟ because the drug kidney
+
+682
+00:52:22,030 --> 00:52:25,390
+agent contains cyanide لإنه بيحتوي على ال cyanide
+
+683
+00:52:25,390 --> 00:52:31,290
+المادة هذه ثامة، خانقة، بتأدي إلى توقف أو
+
+684
+00:52:31,290 --> 00:52:36,670
+inhibition لإنزيمات ال glycolysis و ال creep cycle
+
+685
+00:52:36,670 --> 00:52:41,810
+لإنزيمات التنفس الخلوي، عمليات الايد والميتابوليزم
+
+686
+00:52:41,810 --> 00:52:47,390
+اللي موجودة في الخلاياتمام؟ فبالتالي سامة و قاتلة
+
+687
+00:52:47,390 --> 00:52:50,630
+و طبعا irreversible يا بنات بتعمل inhibition
+
+688
+00:52:50,630 --> 00:52:54,730
+irreversible و لعلكم أخدتوا الإنزيمات و ال
+
+689
+00:52:54,730 --> 00:52:58,370
+inhibitors و النوع ال reversible و ال irreversible
+
+690
+00:52:58,370 --> 00:53:01,470
+تبعها فال cyanide أحد ال irreversible inhibitors
+
+691
+00:53:01,470 --> 00:53:06,750
+لل cell respiratory enzymes تمام؟ و إذا هيقدر إلى
+
+692
+00:53:06,750 --> 00:53:09,790
+اختناق فقتل يعني أربعة liter من هذا ال reagent
+
+693
+00:53:09,790 --> 00:53:14,750
+ممكن تكون قاتلة تناولهافبالتالي ناخد حضارنا منه
+
+694
+00:53:14,750 --> 00:53:17,810
+ايضا الى انه ممكن انها تعمل irritation ل ال skin
+
+695
+00:53:17,810 --> 00:53:23,010
+مش لازم يجي على ال ايدي فلازم انا اكون بستخدم يعني
+
+696
+00:53:23,010 --> 00:53:29,290
+لابسة ل gloves لابسة ال lab coat تبعي يعني دايرة
+
+697
+00:53:29,290 --> 00:53:32,650
+بالي احلي ملتزمة بكل الإجراءات السلامة قبل البدء
+
+698
+00:53:32,650 --> 00:53:36,590
+في العمل acid-free sinks should be used for
+
+699
+00:53:36,590 --> 00:53:44,720
+disposal of this reagent لازم انا استخدمأو أتخلص
+
+700
+00:53:44,720 --> 00:53:49,780
+من هذا ال reagent بأحواض يعني الحوض أو ال .. ال
+
+701
+00:53:49,780 --> 00:53:54,480
+sink .. يعني الحوض الحنفية أنه يكون هذا لا .. يعني
+
+702
+00:53:54,480 --> 00:53:57,900
+أنا مابسكبش فيه acids يعني مابيتنضفش ب acids أو
+
+703
+00:53:57,900 --> 00:54:02,580
+مابسكبش فيه acidsليش؟ لإنه إذا أنا كبيت المحلول
+
+704
+00:54:02,580 --> 00:54:05,280
+اللي هو فيه cyanide وبعدين جيت روحت مضفت بchlor أو
+
+705
+00:54:05,280 --> 00:54:09,720
+ب acid أو كبيت acid فمعناه ال acid هيتراح يروح
+
+706
+00:54:09,720 --> 00:54:12,440
+يتفاعل مع ال cyanide و هيكوملي ال hydrogen cyanide
+
+707
+00:54:12,440 --> 00:54:21,020
+gas اللي هو عبارة عن toxic gas، تمام؟ غاز سامفيفضل
+
+708
+00:54:21,020 --> 00:54:24,480
+انه انا كمان بعد ما انا اتخلص مثلا الانابيب
+
+709
+00:54:24,480 --> 00:54:27,660
+تابعتنا اشتغلنا فيها خلصناها مينفع .. يعني انبوب
+
+710
+00:54:27,660 --> 00:54:30,420
+مليان فيه خمس أمقال من ال reagent يعني كمية اللي
+
+711
+00:54:30,420 --> 00:54:33,760
+بقصبها مينفعش انا اكبها بالسلة فبالتالي بروح انا
+
+712
+00:54:33,760 --> 00:54:37,560
+بطلق .. بكب محتوها بالحوت بعدين بتخلص من الانابيب
+
+713
+00:54:37,560 --> 00:54:43,620
+فإذا ..لازم انه انا افتح الحنفية شوي اسيب من الماء
+
+714
+00:54:43,620 --> 00:54:46,740
+الجارى بحيث انه يعمللي dilution لهذا ال reagent
+
+715
+00:54:46,740 --> 00:54:50,960
+انه لو فيما بعد تم إطلاف acid او اتنظف الحوت
+
+716
+00:54:50,960 --> 00:54:56,240
+بchlor او اشى مايروح يتفاعل ال cyanide مع ال acid
+
+717
+00:54:56,240 --> 00:54:59,040
+و يكونلي hydrogen cyanide gas اللى هو toxic gas
+
+718
+00:54:59,570 --> 00:55:03,050
+تمام؟ إذا هتلاقوني هتلاحظوا عليا إنه أول ما بدتوا
+
+719
+00:55:03,050 --> 00:55:06,390
+تطلفوا أو إنه تعملوا discard لمحتوى القنابيب
+
+720
+00:55:06,390 --> 00:55:10,270
+تلاقوني فاتحت الحنافية و سبتها مفتوحة ماحدش يسكرها
+
+721
+00:55:10,270 --> 00:55:14,290
+لإنها بديها تعمل dilution لها هذا ال agent بحيث
+
+722
+00:55:14,290 --> 00:55:20,770
+إنه ما يكون عم بنشكل أو بننتج غاز سام إنه بعد هيك
+
+723
+00:55:20,770 --> 00:55:26,140
+أي حد يفوت على المختبر يتأذىهك بنكون انتهانا من
+
+724
+00:55:26,140 --> 00:55:29,700
+محاضرتنا لليوم يعطيكم ألف عافية ونراكم في المعمل
+
+725
+00:55:29,700 --> 00:55:30,360
+للتطبيق
+
diff --git a/PL9fwy3NUQKwY9jl8xw-bWkVZkWcGGyspd/fqKeQtgENUo.srt b/PL9fwy3NUQKwY9jl8xw-bWkVZkWcGGyspd/fqKeQtgENUo.srt
new file mode 100644
index 0000000000000000000000000000000000000000..4933f6e2cfe179a76109743e8dd1faad69b2d8b3
--- /dev/null
+++ b/PL9fwy3NUQKwY9jl8xw-bWkVZkWcGGyspd/fqKeQtgENUo.srt
@@ -0,0 +1,2254 @@
+1
+00:00:10,530 --> 00:00:14,590
+السلام عليكم ورحمة الله وبركاته طالبات العزيزات
+
+2
+00:00:14,590 --> 00:00:19,590
+اليوم إن شاء الله رح نكمل موضوعنا المفروض يعني هو
+
+3
+00:00:19,590 --> 00:00:26,370
+يعني يندرج تحت العملي عن ال laboratory safety عن
+
+4
+00:00:26,370 --> 00:00:32,030
+قواعد السلامة والأمان، الصراحة المحاضرة اليوم فيها
+
+5
+00:00:32,030 --> 00:00:38,150
+يعني ملاحظات كثيرة، فيها كلام كثير ولكن الـ الاشيء
+
+6
+00:00:38,150 --> 00:00:45,330
+مفهوم يعني، وهو ممكن أنتن يعني تحسي حالك زهقتي من
+
+7
+00:00:45,330 --> 00:00:49,030
+الكلام بس في النهاية أنتي يعني أنا .. أنا بتخيل أنه
+
+8
+00:00:49,030 --> 00:00:54,530
+إنك تسمعي لمرة واحدة أو مرتين راح يرسخ يعني هو مش
+
+9
+00:00:54,530 --> 00:00:58,690
+صعب، بس إنما هو كلام وحتى هيكون مكرر يعني في جزئية
+
+10
+00:00:58,690 --> 00:01:04,330
+منيح يعني أنه شرحناها أو غطيناها خلال المحاضرة
+
+11
+00:01:04,330 --> 00:01:09,130
+النظرية السابقة، فالكلام يعني هيكون في جزء جديد
+
+12
+00:01:09,130 --> 00:01:13,210
+عليكم في جزء سمعتيه قبل هيك، فأتمنى إن شاء الله
+
+13
+00:01:13,210 --> 00:01:16,950
+يعني تكون كل الأمور تمام وكلها واضحة، طبعا احنا
+
+14
+00:01:16,950 --> 00:01:21,330
+حكينا في الـ في المختبر، طبعا أنا ليش بحافظ على
+
+15
+00:01:21,330 --> 00:01:25,710
+قواعد السلامة والأمان علشان الـ safe working
+
+16
+00:01:25,710 --> 00:01:30,070
+الشغل، الأمن بيحمي مين؟ بيحمي نفسي و الـ other lab
+
+17
+00:01:30,070 --> 00:01:34,430
+workers، زملائي و الـ cleaners، الـ الناس عمال
+
+18
+00:01:34,430 --> 00:01:37,870
+النظافة اللي بيشرفوا على نظافة المكان، الـ visitors
+
+19
+00:01:37,870 --> 00:01:42,850
+زوار المكان، ويعني المرضى اللي بيزوروا المكان، و
+
+20
+00:01:42,850 --> 00:01:46,610
+أيضا شغلي، طالما أنا إذا كنت بشتغل شغل safe
+
+21
+00:01:46,610 --> 00:01:51,330
+شغل آمن، إذا هيكون شغلي صحيح تمام، وهحصل على نتائج
+
+22
+00:01:51,330 --> 00:01:56,890
+صحيحة تفيد في الـ diagnosis، حكينا
+
+23
+00:01:56,890 --> 00:02:00,570
+هذا هذا الكلام، معاد حكينا قبل هيك أنه لازم
+
+24
+00:02:00,570 --> 00:02:04,930
+أحافظ على ارتداء الـ lab coat تمام، ولازم الـ long
+
+25
+00:02:04,930 --> 00:02:09,250
+hair يكون مرفوع، الشعر اللي طويل .. sorry
+
+26
+00:02:12,690 --> 00:02:18,330
+الشعر لازم يكون مرفوع، وطبعا ما نلبسش ملابس واسعة
+
+27
+00:02:18,330 --> 00:02:22,230
+ولاازم الـ shoes يكون closed مش يكون مفتوح، الـ
+
+28
+00:02:22,230 --> 00:02:26,750
+sandals والأشياء المفتوحة هاي نروح .. نحاول أنه
+
+29
+00:02:26,750 --> 00:02:30,840
+ما ناخدها، تمام؟ وبالنسبة كمان أيضا لازم أرتدي لـ
+
+30
+00:02:30,840 --> 00:02:33,260
+gloves، لـ gloves، أنه أنا في عندي نوعين من الـ
+
+31
+00:02:33,260 --> 00:02:36,520
+gloves، في الـ gloves اللي هي مصنوعة من الـ latex
+
+32
+00:02:36,520 --> 00:02:40,540
+اللي زي الـ nylon هذا، هذا اللي احنا بنستخدمه للشغل
+
+33
+00:02:40,540 --> 00:02:44,720
+طبعنا، طبعا فيه منه أنواع وفيه منه درجات حتى في
+
+34
+00:02:44,720 --> 00:02:48,960
+نسبة الـ protection، يعني في بيحمي أكثر، في بيحمي أقل
+
+35
+00:02:49,170 --> 00:02:55,070
+فرح ناخدهم طبعا بالتفصيل لقدام شوية، وأيضا في الـ
+
+36
+00:02:55,070 --> 00:02:59,890
+gloves السميك اللي مصنوع من النسيج أو القماش اللي
+
+37
+00:02:59,890 --> 00:03:07,210
+هو للتعامل مع الأشياء الساخنة، طبعا في قاعدة بتحكيلك
+
+38
+00:03:07,210 --> 00:03:09,610
+remove your gloves before using an instrument,
+
+39
+00:03:09,830 --> 00:03:12,490
+telephone and leaving the laboratory، طبعا قبل أن
+
+40
+00:03:12,490 --> 00:03:16,830
+أستخدم أي جهاز وأنا بتعامل مع العين أو المحاليل و
+
+41
+00:03:16,830 --> 00:03:19,410
+الأدوات لازم أكون لابسة gloves، لما أجد أستخدم جهاز
+
+42
+00:03:19,410 --> 00:03:22,510
+أو حتى الـ telephone تبعي أو ألمس أغراضي أو أغادر
+
+43
+00:03:22,510 --> 00:03:25,890
+من المختبر لازم أكون تخلصت من الـ gloves وألقيها
+
+44
+00:03:25,890 --> 00:03:30,420
+علشان ما أنشر الـ contamination everywhere، طبعا لو
+
+45
+00:03:30,420 --> 00:03:34,100
+انسكب أي مادة كيميائية على الجلد لازم أنا أغسلها
+
+46
+00:03:34,100 --> 00:03:38,420
+بالماء لمدة ربع ساعة، لازم أغسل يديّ قبل وبعد
+
+47
+00:03:38,420 --> 00:03:44,140
+المختبر، وأيضا الـ الـ الـ هو أنا مش عارفة
+
+48
+00:03:44,140 --> 00:03:51,710
+من اللي عاملة عرض فهو العرض يعني غريب، طيب، وبحكيلك
+
+49
+00:03:51,710 --> 00:03:55,450
+هنا handle a glassware sharp tool and heated
+
+50
+00:03:55,450 --> 00:04:00,030
+containers carefully، تعاملي مع المواد الزجاجية،
+
+51
+00:04:00,030 --> 00:04:04,690
+الأدوات الحادة، والأدوات الساخنة بحذر
+
+52
+00:04:09,980 --> 00:04:13,420
+بنتعامل مع .. بنستخدم الـ safety goggles، النضارات
+
+53
+00:04:13,420 --> 00:04:17,520
+لو أنا بستخدم مادة كيميائية لها الـ يعني لها
+
+54
+00:04:17,520 --> 00:04:25,220
+بمتطاير أو رذاذ متطاير أو حتى يعني depress اللي هي
+
+55
+00:04:25,220 --> 00:04:30,040
+يعني بقايا متطايرة ممكن تلامس عيوني، أنا بتخلص
+
+56
+00:04:30,040 --> 00:04:37,940
+منها، إذا كان طبعا أي أحد بيرتدي عدسات لاصقة لازم
+
+57
+00:04:37,940 --> 00:04:43,120
+يخبر الدكتور، وأيضا يعني يخبر المدرس اللي موجود في
+
+58
+00:04:43,120 --> 00:04:48,560
+المختبر، وأيضا في حالة الـ الـ emergency، لو يعني
+
+59
+00:04:48,560 --> 00:04:51,860
+في حالة الطوارئ، لو أي شيء، أي chemical دخلت إلى
+
+60
+00:04:51,860 --> 00:04:55,240
+العين، لازم أنا أغسلها من خلال الـ washer تبع العين
+
+61
+00:04:55,240 --> 00:04:58,380
+لمدة ربع ساعة، واحنا حكينا عن الـ washer هذه
+
+62
+00:04:58,380 --> 00:05:01,880
+المحاضرة الماضية، طبعا إيش الأخطار اللي ممكن تقابلني
+
+63
+00:05:01,880 --> 00:05:06,000
+داخل المختبر؟ اللي هي خطر حدوث حرائق، خطر إيه يعني
+
+64
+00:05:06,000 --> 00:05:10,520
+كسر لمادة زجاجية، الأدوات الحادة، خطر انسكاب أي
+
+65
+00:05:10,520 --> 00:05:16,960
+مادة كيميائية، خطر الغاز والسوائل المضغوطة، وخطر
+
+66
+00:05:16,960 --> 00:05:21,180
+الحرارة والبرودة، طالما أن الحرارة العالية و
+
+67
+00:05:21,180 --> 00:05:25,200
+البرودة العالية ممكن تسبب حروق للجسم، خطر المواد
+
+68
+00:05:25,200 --> 00:05:28,080
+الكيميائية والمواد البيولوجية زي الفيروسات
+
+69
+00:05:28,080 --> 00:05:30,780
+والبكتيريا والفطريات والطفيليات اللي بتكون موجودة
+
+70
+00:05:30,780 --> 00:05:34,920
+داخل المختبر، الإشعاع، طيب وأي أنواع أخرى من
+
+71
+00:05:34,920 --> 00:05:38,920
+المخاطر؟ لو كان في أنتي .. أنا كيف بدي أتجنب حدوث
+
+72
+00:05:38,920 --> 00:05:42,760
+حرائق؟ أول حاجة المادة اللي بسميها flammable، إيش
+
+73
+00:05:42,760 --> 00:05:47,600
+يعني flammable؟ يعني مادة قابلة للاشتعال تمام أو مشتعلة طبعا
+
+74
+00:05:47,600 --> 00:05:50,800
+بدي أـ لازم يكون في عندي منها في المختبر كميات
+
+75
+00:05:50,800 --> 00:05:55,620
+قليلة، ما أقدرش أخزنها بكميات كبيرة، تمام؟ store in
+
+76
+00:05:55,620 --> 00:05:59,300
+special storage cabinet، طبعا بحطها في كابينات خاصة
+
+77
+00:05:59,300 --> 00:06:04,220
+بيبقى محافظة على برودتها، إنها يعني موفرة لي درجة
+
+78
+00:06:04,220 --> 00:06:08,980
+حرارة .. حرارة باردة، عشان هاي المادة ما تشتعل، و
+
+79
+00:06:08,980 --> 00:06:11,820
+طبعا لازم أستخدم temperature controlled heating
+
+80
+00:06:11,820 --> 00:06:16,260
+source، يعني .. يعني يكون في عندي قياس مستمر لـ
+
+81
+00:06:16,290 --> 00:06:21,790
+الدرجة الحرارة داخل هاي الـ cabinet، عشان ما
+
+82
+00:06:21,790 --> 00:06:26,650
+يكون في عندي ارتفاع لدرجات الحرارة ممكن يسبب
+
+83
+00:06:26,650 --> 00:06:30,830
+اشتعالها، حتى لما بدي أتعامل مع هاي المادة اللي
+
+84
+00:06:30,830 --> 00:06:34,750
+بنسميها فيلم بالمشتعلة، زي الكحول مثلا، لازم بدي
+
+85
+00:06:34,750 --> 00:06:38,150
+أسخنها بأستخـ بسخنها باستخدام الـ water bath بدل
+
+86
+00:06:38,150 --> 00:06:41,810
+ما أحطها مباشرة على النار وتشتعل، لأ بأستخدمها ..
+
+87
+00:06:41,810 --> 00:06:44,430
+بسخنها باستخدام water bath، في كتير من المواد
+
+88
+00:06:44,430 --> 00:06:49,870
+بنسميها flammable، مش بس كحول، أحيانا الـ ether، الـ
+
+89
+00:06:49,870 --> 00:06:55,950
+يعني .. الـ Acetone، كتير مواد، هنتعامل معها، بعض الـ
+
+90
+00:06:55,950 --> 00:07:00,510
+acids، هنتعامل .. نتعامل معهم، بيعطينا تحذير أنه هذه
+
+91
+00:07:00,510 --> 00:07:03,230
+المادة flammable، لما بدي أسخنها بسخنها باستخدام
+
+92
+00:07:03,230 --> 00:07:06,670
+water bath، يعني حمام مائي، أفضل من ما أعرضها لدرجة
+
+93
+00:07:06,670 --> 00:07:10,810
+الحرارة مباشرة، وبتتخزن بـ cabinet، وبنستخدم منها
+
+94
+00:07:10,810 --> 00:07:14,350
+كميات قليلة، تمام؟ بتكون عندي بدل ما أنا أستخدم
+
+95
+00:07:14,350 --> 00:07:17,970
+tank داخل المختبر من الكحول مثلا، لأ أنا بـ بـ
+
+96
+00:07:17,970 --> 00:07:21,950
+بخليها في المخزن وبجيب على المختبر bottles، على قدر
+
+97
+00:07:21,950 --> 00:07:26,490
+استخدامي وحاجتي، make sure that you know what to
+
+98
+00:07:26,490 --> 00:07:30,510
+do، طبعا إذا حدث حريق لازم تكوني تعرفي تعملي ايه،
+
+99
+00:07:30,510 --> 00:07:34,740
+if you have a fire، if you hear a fire alarm، إذا أنتي
+
+100
+00:07:34,740 --> 00:07:39,940
+كان في عندك حريق أو كان في عندك إنذار لحدوث حريق
+
+101
+00:07:39,940 --> 00:07:43,620
+لازم تكوني بتعرفي إيش بدك تعملي، كيف بدك تتصرفي،
+
+102
+00:07:43,620 --> 00:07:48,160
+كيف يعني؟ يعني مثلا بتعرفي مكان الـ fire الـ fire
+
+103
+00:07:48,160 --> 00:07:51,580
+fighting equipment، تعرفي مكان أدوات اللي هي
+
+104
+00:07:51,580 --> 00:07:55,860
+مُحاربة حدوث حرائق زي الـ blanket البطانية، أعرف
+
+105
+00:07:55,860 --> 00:08:01,400
+إنها موجودة داخل المختبر، النبوبة .. النبوبة لإطفاء
+
+106
+00:08:01,400 --> 00:08:06,220
+الحرائق، أيضا كمان أعرف إن موجودة في عندي أدوات
+
+107
+00:08:06,220 --> 00:08:09,940
+برضه بستخدمها لإطفاء أنواع كثيرة من الحرائق زي
+
+108
+00:08:09,940 --> 00:08:15,080
+الرمل والـ والأنابيب مي، وأشياء هذه، ده كنتي عارفة
+
+109
+00:08:15,080 --> 00:08:15,900
+إنها موجودة
+
+110
+00:08:19,070 --> 00:08:23,590
+وأيضا غير أن أنا أبدأ أكون عارفة وين موجودة، بدي
+
+111
+00:08:23,590 --> 00:08:27,270
+أكون عارفة مثلا مخرج الطوارئ من وين، كل مختبر
+
+112
+00:08:27,270 --> 00:08:31,630
+بيكون إيه له في الخارج مخرج .. مخرج طوارئ موجود
+
+113
+00:08:31,630 --> 00:08:37,890
+فيه، فبالتالي لازم نكون بنعرف كيف نستخدمه، تمام؟
+
+114
+00:08:37,890 --> 00:08:42,490
+هذا بالنسبة لي، الجملة اللي معناها know the location
+
+115
+00:08:42,490 --> 00:08:47,950
+of firefighting equipment، طيب طبعا في عندي كل نوع
+
+116
+00:08:47,950 --> 00:08:52,070
+حرائق أنا بستخدم لها مادة لإطفائها، يعني مثلا الـ
+
+117
+00:08:52,070 --> 00:08:55,250
+bucket of water، بيكون في عندي دلاء للماء معـ
+
+118
+00:08:55,250 --> 00:08:58,970
+معبقة بالماء، طبعا هادي بتستخدم to extinguish
+
+119
+00:08:58,970 --> 00:09:03,870
+paper and wood fire، لـ لحتى أطفئ الحرائق اللي
+
+120
+00:09:03,870 --> 00:09:07,270
+ناتجة من اشتعال ورق أو خشب، لو بنش خشب .. طبعا ..
+
+121
+00:09:07,710 --> 00:09:10,930
+يعني شبكت فيه نار وهي بدأت .. بدأت تحترق، أو أوراق
+
+122
+00:09:10,930 --> 00:09:14,950
+على طول بروح أشـ بجيب الـ buckets of water وبفتحها
+
+123
+00:09:14,950 --> 00:09:22,390
+وبكبها عليها، تمام؟ فبالتالي الـ يعني الماء هي
+
+124
+00:09:22,390 --> 00:09:27,270
+مستحيل أستخدمها لحتى أطفئ حرائق ناتجة عن كهرباء، بل
+
+125
+00:09:27,270 --> 00:09:30,170
+أنها هي هتسبب انفجار يعني أو هتـ هتعمل mass
+
+126
+00:09:30,170 --> 00:09:34,970
+كهربائي، فبالتالي يعني كل مادة تستخدم في نوع من
+
+127
+00:09:34,970 --> 00:09:38,440
+أنواع الحرائق، الـ bucket of sand، دلاء بيكون فيها
+
+128
+00:09:38,440 --> 00:09:43,660
+رمل، طبعا هذا الرمل أو حتى تربة جافة dry soil، طبعا
+
+129
+00:09:43,660 --> 00:09:52,300
+تستخدم للحرائق اللي ناتجة من اشتعال السوائل
+
+130
+00:09:52,300 --> 00:09:57,080
+المشتعلة، يعني لو كحول، طبعا سائل هذا الكحول اشتعل
+
+131
+00:09:57,080 --> 00:10:00,620
+على طول بدي أروح على .. يعني انسكب على البنش و
+
+132
+00:10:00,620 --> 00:10:04,020
+اشتعل فبدي أروح على طول أحط عليه bucket of sand
+
+133
+00:10:04,020 --> 00:10:07,540
+يعني أفتح الدلو كده، وأحط الرمل وأكبه عليه علشان
+
+134
+00:10:07,540 --> 00:10:14,400
+يطفي، fire blanket طبعا اللي هي البطانية المصنوعة من
+
+135
+00:10:14,400 --> 00:10:19,650
+الـ heavy cotton، طبعا هي عبارة عن حاجة .. هي يعني
+
+136
+00:10:19,650 --> 00:10:22,610
+المادة اللي .. اللي مصنوعة منها هاي الـ blankets
+
+137
+00:10:22,610 --> 00:10:26,910
+البطانيات fire retardant chemicals، يعني من مواد
+
+138
+00:10:26,910 --> 00:10:34,370
+كيميائية يعني معيقة لنشوب الحرائق أو يعني إنها
+
+139
+00:10:34,370 --> 00:10:40,810
+طاردة للحرائق أو للنار، فبالتالي بنستخدمها لما ..
+
+140
+00:10:43,410 --> 00:10:48,170
+بنطفئ الحرائق سواء كانت يعني اشتعلت بالجسم أو لأ،
+
+141
+00:10:48,170 --> 00:10:54,650
+تمام؟ واسمها إيه؟ الـ dry powder chemical، إذا طبعا في
+
+142
+00:10:54,650 --> 00:10:58,950
+دلاء بيكون فيها dry powder، مواد powder كيميائية
+
+143
+00:10:58,950 --> 00:11:02,570
+بتستخدم علشان أطفئ فيها الحرائق اللي ناتجة من
+
+144
+00:11:02,570 --> 00:11:07,330
+اشتعال جهاز كهربائي أو حريق ناتج عن كهرباء، تمام؟
+
+145
+00:11:07,330 --> 00:11:10,990
+إذا أنتي عندك الـ الـ bucket of water علشان أطفئ
+
+146
+00:11:11,130 --> 00:11:15,230
+الـ الحرائق المشتعلة في الـ paper والـ والـ
+
+147
+00:11:15,230 --> 00:11:21,170
+wood الخشب، والـ sand للحرائق اللي مشتعلة في سوائل
+
+148
+00:11:21,170 --> 00:11:25,090
+والـ fire blanket لو كانت الحرائق لمست جسمي أو
+
+149
+00:11:25,090 --> 00:11:31,590
+ملابسي، والـ dry powder للحرائق الناتجة من التيار
+
+150
+00:11:31,590 --> 00:11:37,400
+الكهربائي، تمام؟ طبعا عشان أنا أحمي نفسي من .. وأنا
+
+151
+00:11:37,400 --> 00:11:42,480
+بشتغل مع الـ الـ لها بنزن أو مع أي جهاز
+
+152
+00:11:42,480 --> 00:11:47,320
+بيعطيني حرارة أو بيعطي fire، فبالتالي لازم أنا أربط
+
+153
+00:11:47,320 --> 00:11:51,320
+شعري للأعلى سواء كان للناس اللي مش محجبات، يعني أنا
+
+154
+00:11:51,320 --> 00:11:54,780
+حاكية إن احنا محجبات وما عندنا هاي المشكلة طبعا، و
+
+155
+00:11:54,780 --> 00:11:59,000
+أنا بسخن في المحلول إذا كان .. لجأت لتسخين محلول
+
+156
+00:11:59,330 --> 00:12:03,070
+never look into container، ما أبُصّش في المحلول نفسه، و
+
+157
+00:12:03,070 --> 00:12:06,290
+هو بيغلي لأنّه ممكن على طول يفور ويـ ويلمس
+
+158
+00:12:06,290 --> 00:12:10,530
+وجهي، never point the end of test tube being heated
+
+159
+00:12:10,530 --> 00:12:15,530
+to yourself or other، يعني مثلا أنا بسخن في تيوب
+
+160
+00:12:15,530 --> 00:12:20,850
+على الـ اللهب، مش لازم فتحة التيوب تكون موجّهة في
+
+161
+00:12:20,850 --> 00:12:24,910
+اتجاهي أو باتجاه زميلتي، لأ، باتجاه الحائط، لازم تكون
+
+162
+00:12:24,910 --> 00:12:29,470
+موجّهة، تمام؟ never heat in a closed container
+
+163
+00:12:29,470 --> 00:12:32,630
+ما أسخّنش اللي أنا بيبقى وهي مغلقة لأنها ممكن تنفجر
+
+164
+00:12:32,630 --> 00:12:33,250
+وتنكسر
+
+165
+00:12:37,010 --> 00:12:41,290
+طيب، never leave a heat source unattended، يعني
+
+166
+00:12:41,290 --> 00:12:45,330
+ما تسيبيش مصدر الحرارة أو مصدر اللهب متروك بدون
+
+167
+00:12:45,330 --> 00:12:48,290
+انتباه، يعني احنا حكينا المحاضرة الماضية ممنوع أترك
+
+168
+00:12:48,290 --> 00:12:52,950
+لهب بنزن بدون يضل شغال وأروح أشوف شغل ثاني أو
+
+169
+00:12:52,950 --> 00:12:56,090
+أشتغل في مكان ثاني، لأ، بأخلص، بأقفل لهب بنزين وبأؤمن
+
+170
+00:12:56,090 --> 00:13:00,150
+الغاز، بعدين بنتقل إلى مكان آخر، heated metal and
+
+171
+00:13:00,150 --> 00:13:05,730
+glass looks cool use forceps or gloves before
+
+172
+00:13:05,730 --> 00:
+
+223
+00:16:49,990 --> 00:16:54,630
+وقتها dispose of any hazardous material and toxic
+
+224
+00:16:54,630 --> 00:16:58,870
+waste و المادة اللي .. اللي انكبت أو انكسرت بتخلص
+
+225
+00:16:58,870 --> 00:17:02,690
+منها كأنها على أساس أنها هي toxic، طبعا الـ messy
+
+226
+00:17:02,690 --> 00:17:05,840
+workers اللي .. اللي ..الناس اللي بتشتغل بفوضى
+
+227
+00:17:05,840 --> 00:17:11,100
+دايما هم عبارة عن poor workers، يعني شغلهم ما ..
+
+228
+00:17:11,100 --> 00:17:15,060
+مافيش إنتاجية، مافيش نتائج دقيقة، مافيش شيء صحيح
+
+229
+00:17:15,060 --> 00:17:17,900
+فبالتالي هذه مشكلتهم
+
+230
+00:17:20,330 --> 00:17:25,770
+طبعا خطر وجود استوانات الغاز أو السوائل المضغوطة
+
+231
+00:17:25,770 --> 00:17:29,310
+داخل .. أو الغازات المضغوطة داخل المختبر أول حاجة
+
+232
+00:17:29,310 --> 00:17:32,510
+طبعا ممنوع أن أستخدم هذه الاستوانات بدون ما أكون
+
+233
+00:17:32,510 --> 00:17:36,470
+متدرب عليها، و لازم أقلل عددها، minimize the number
+
+234
+00:17:36,470 --> 00:17:40,350
+in the laboratory، أقلل عددها داخل المختبر الـ
+
+235
+00:17:40,350 --> 00:17:45,990
+cylinders، طبعا هي عبارة عن شيء ثقيل، فبالتالي ما بنفع
+
+236
+00:17:45,990 --> 00:17:50,080
+إذا سقطت ممكن يعني على رجلي أو شيء ممكن تعمل
+
+237
+00:17:50,080 --> 00:17:53,960
+أذى كبير، فبالتالي لازم أنا لما أجي أحركها من
+
+238
+00:17:53,960 --> 00:17:57,380
+مكان إلى مكان ما أستخدم العضلات، إنما أستخدم
+
+239
+00:17:57,380 --> 00:18:04,520
+literally، يعني أروح أجيب الـ .. يعني أداة بـ ..
+
+240
+00:18:04,520 --> 00:18:10,200
+بيلها عجلات بتتحرك، و بـ .. بحط الـ cylinder عليها، و
+
+241
+00:18:10,200 --> 00:18:13,540
+بنقلها من مكان لآخر تبع .. باستخدام literally
+
+242
+00:18:13,540 --> 00:18:18,700
+تمام؟ ensure that they are connected when in use.
+
+243
+00:18:18,700 --> 00:18:22,100
+طبعا لازم أنا أتأكد أنها موصلة منيح أثناء
+
+244
+00:18:22,100 --> 00:18:25,740
+استخدامها، ما .. يعني ما يكونش في أشياء فالتة أو
+
+245
+00:18:25,740 --> 00:18:31,960
+أشياء رخوة، لأ، لازم تكون محكمة الإغلاق، و هي محكومة
+
+246
+00:18:31,960 --> 00:18:38,160
+الاتصال بالكوابل تبعتها، أو اللي هي بالأنابيب
+
+247
+00:18:38,160 --> 00:18:42,640
+تبعتها، use a regulator، طبعا لازم يكون في عندي منظم
+
+248
+00:18:42,640 --> 00:18:46,960
+لها عشان يتحكم لي بكمية الغاز اللي بتطلع، و أيضا
+
+249
+00:18:46,960 --> 00:18:51,140
+إذا كان في عندي أي مشكلة أو أي تسريب لازم أنا
+
+250
+00:18:51,140 --> 00:18:55,120
+أعالج المشكلة بـ .. بـ .. باستمرار، لأن حكينا تسريب
+
+251
+00:18:55,120 --> 00:18:56,600
+الغاز ممكن يعمل حرائق.
+
+252
+00:18:59,800 --> 00:19:04,860
+Cryogenics، في عندي غازات طبعا هي liquid، أنا بضغطها
+
+253
+00:19:04,860 --> 00:19:10,840
+لحتى أحافظ، لحتى أخزن مثلا أشياء على درجات حرارة
+
+254
+00:19:10,840 --> 00:19:16,880
+كتير كتير قليلة، يعني مثلا الخلايا جسم الإنسان لما
+
+255
+00:19:16,880 --> 00:19:20,120
+أنا بدي أخزنها، بخزنها على سالب سبعين و سالب ثمانين
+
+256
+00:19:20,120 --> 00:19:25,950
+طبعا ما بوصلش لدرجات الحرارة هذه إلا باستخدام غازات
+
+257
+00:19:25,950 --> 00:19:30,310
+طبعا الـ liquid gases، طبعا liquid gases اللي هي
+
+258
+00:19:30,310 --> 00:19:35,130
+عبارة عن هي extremely cold، بتكون باردة كتير، و ممكن
+
+259
+00:19:35,130 --> 00:19:38,610
+تسبب حرائق، في حرائق بسبب البرودة زي ما في حرائق
+
+260
+00:19:38,610 --> 00:19:41,390
+بسبب ارتفاع درجات الحرارة، أو اللي هي عند الـ heat
+
+261
+00:19:41,390 --> 00:19:46,050
+السخونة، liquid gas can evaporate، ممكن أن هذه الـ
+
+262
+00:19:46,050 --> 00:19:49,810
+liquid gases تتبخر و تعمل لي اختناق
+
+263
+00:19:49,810 --> 00:19:53,010
+فبالتالي لازم أنا أحافظ عليها أن هي ما تتبخر و ما
+
+264
+00:19:53,010 --> 00:19:58,070
+تتسرب، تكون محكمة الإغلاق داخل الأنابيب تبعتها، و
+
+265
+00:19:58,070 --> 00:20:01,630
+طبعا ما بستخدمها إلا بـ training خاص فيها، زي الـ
+
+266
+00:20:01,630 --> 00:20:07,230
+liquid nitrogen، أكيد سمعتوا عنه، بعدين ممكن أن
+
+267
+00:20:07,230 --> 00:20:11,130
+تكون قابلة للانفجار أو الاحتراق، الـ electrical
+
+268
+00:20:11,130 --> 00:20:16,510
+equipment، طبعا كيف أنا أتعامل، الحد من خطر درجات
+
+269
+00:20:16,510 --> 00:20:22,950
+أو خطر الكهرباء، أول حاجة بدي أعمل visual check على
+
+270
+00:20:22,950 --> 00:20:25,790
+الـ electrical equipment، حكينا المحاضرة الماضية أن
+
+271
+00:20:25,790 --> 00:20:28,570
+الأدوات الكهربائية لازم باستمرار أنا أشيك عليها
+
+272
+00:20:28,820 --> 00:20:33,800
+أفحص نظرة عليها، أشوف درجة حرارتها، أشوف هل هي
+
+273
+00:20:33,800 --> 00:20:37,160
+مشبوكة، هل هي بتشتغل بانتظام أو لأ، فيها خلل أو
+
+274
+00:20:37,160 --> 00:20:41,120
+فيها عطل، بتعطيني alarm، يعني إنذار أن والله أن
+
+275
+00:20:41,120 --> 00:20:44,740
+هي .. هو هذا الجهاز خربان، فهذا كله لازم أكون
+
+276
+00:20:44,740 --> 00:20:49,600
+متعاملة معاه، بعدين كمان، all portable electrical
+
+277
+00:20:49,600 --> 00:20:53,340
+equipment must have a current path test، يعني لازم
+
+278
+00:20:53,340 --> 00:20:58,930
+يكون فيه على كل الـ .. الأجهزة الكهربائية عبارة عن
+
+279
+00:20:58,930 --> 00:21:02,410
+sticker، بسميه portable appliance testing، يعني هذا
+
+280
+00:21:02,410 --> 00:21:05,730
+الـ sticker بيحكي لي أن هو شغال ولا مش شغال، إذا كان
+
+281
+00:21:05,730 --> 00:21:11,370
+فيه مشكلة أو لأ، إمتى تم تشغيله، طبعا إيش محتاج
+
+282
+00:21:11,370 --> 00:21:14,130
+ملاحظات أثناء تشغيله، هذه كلها بتكون موجودة على الـ
+
+283
+00:21:14,130 --> 00:21:17,830
+portable appliance testing sticker، طبعا never use
+
+284
+00:21:17,830 --> 00:21:21,110
+defective equipment، إذا الجهاز مكتوب لك عليه أن هو
+
+285
+00:21:21,110 --> 00:21:26,400
+هذا defective، فيه خلل، عطل major، تستخدميه نهائيا
+
+286
+00:21:26,400 --> 00:21:31,560
+ما تحاوليش فيه، تمام؟ و طبعا only electrical plugs
+
+287
+00:21:31,560 --> 00:21:34,840
+are to be placed into electrical outlets، طبعا بس
+
+288
+00:21:34,840 --> 00:21:38,060
+الـ أسلاك الكهربائية هي اللي إحنا، الكبش هي
+
+289
+00:21:38,060 --> 00:21:44,570
+اللي بنحطها داخل الـ .. يعني داخل الطيار الكهربائي
+
+290
+00:21:44,570 --> 00:21:51,290
+أما الأدوات الحادة، لأ، أقلام، أشياء تانية، لأ، unplug
+
+291
+00:21:51,290 --> 00:21:54,570
+electrical equipment after use، بعد ما أخلص استخدام
+
+292
+00:21:54,570 --> 00:21:59,450
+الجهاز لازم طبعا أرفع الكوابل، أفصلها، ما أخليها
+
+293
+00:21:59,450 --> 00:22:03,110
+مشبوكة بالكهربا، و طبعا كل الـ electrical cords
+
+294
+00:22:03,110 --> 00:22:08,080
+الأحبال و الأسلاك الكهربائية لازم تكون بعيدة عن
+
+295
+00:22:08,080 --> 00:22:12,260
+الماء، و لازم طبعا الماء ما يلمسها إذا كانت هي مكشوفة
+
+296
+00:22:12,260 --> 00:22:20,560
+طيب، الـ chemical safety، الـ chemical safety أول حاجة
+
+297
+00:22:20,560 --> 00:22:23,240
+بحكيلك عن المواد الكيميائية، طبعا هتكون عندنا
+
+298
+00:22:23,240 --> 00:22:28,500
+زجاجات كيميائية زي هذا الشكل، بهك، بهذا الشكل، هك
+
+299
+00:22:30,830 --> 00:22:35,650
+طبعا زي ما أنتم شايفين، و هذه الزجاجات عليها
+
+300
+00:22:35,650 --> 00:22:40,430
+علامات، طبعا العلامات هذه، أو يعني الـ label هذا
+
+301
+00:22:40,430 --> 00:22:46,350
+الأوراق الملصقة على الزجاجات
+
+302
+00:22:46,350 --> 00:22:50,810
+فيها معلومات بتخص كل .. كل شيء بيتعلق بالمادة
+
+303
+00:22:50,810 --> 00:22:54,770
+الكيميائية، استخدامها، درجة حرارة حفظها، تاريخ
+
+304
+00:22:54,770 --> 00:22:59,970
+فتحها، تاريخ انتهاءها، تركيبتها الكيميائية، تعليمات
+
+305
+00:22:59,970 --> 00:23:06,230
+الاستخدام، تحذيرات، استخدامها بشكل خاطئ، كل شيء
+
+306
+00:23:06,230 --> 00:23:09,230
+بيكون موجودة على هذا الـ label، فبالتالي لازم أنا
+
+307
+00:23:09,230 --> 00:23:12,650
+أقرأ الـ label هذا twice، بده أقرأه أكثر من مرة، مرتين
+
+308
+00:23:12,650 --> 00:23:17,850
+عشان أتأكد أن أنا فاهمة كل شيء بيتعلق بهذه المادة
+
+309
+00:23:17,850 --> 00:23:21,790
+قبل استخدامها، طبعا only use the type and amount of
+
+310
+00:23:21,790 --> 00:23:25,490
+chemical instructed، طبعا أنا بأستخدم نفس نوع المادة
+
+311
+00:23:25,490 --> 00:23:30,150
+و نفس الكمية في التفاعل الكيميائي، مثلا حكالي والله
+
+312
+00:23:30,150 --> 00:23:33,950
+في التفاعل الكيميائي ضيفي 2 ml of ether، دور
+
+313
+00:23:33,950 --> 00:23:38,650
+بالخزانة، فتش، فتش، فتش، مافيش ether، لاجت acetone، يلا
+
+314
+00:23:38,650 --> 00:23:41,610
+ماهو ether و acetone إخوان بعض، خليني أضيف
+
+315
+00:23:41,610 --> 00:23:46,390
+acetone، اتنين ML acetone، والله ما أنا مش عاجبني
+
+316
+00:23:46,390 --> 00:23:49,810
+أني أحط اتنين ML، مش هيك، مش حاسة أن النتيجة هتطلع
+
+317
+00:23:49,810 --> 00:23:54,080
+مظبوطة، خليني أحط ثلاثة أمال، هذا خطأ، لازم أستخدم
+
+318
+00:23:54,080 --> 00:23:57,340
+نفس المادة الكيميائية المشار إليها بالتجربة، و نفس
+
+319
+00:23:57,340 --> 00:24:00,980
+الكمية، ما بنفعش أنا هيك، يعني يكون في عندي حس الطبخ
+
+320
+00:24:00,980 --> 00:24:04,820
+و الحس الطباخ، و أحس أني أنا اتنين أمال، لأ لأ مش
+
+321
+00:24:04,820 --> 00:24:11,510
+كافيين، زودوا، لأ ما بنفعش، تمام؟ فـ .. و .. و كمان هذه
+
+322
+00:24:11,510 --> 00:24:14,710
+المعلومة مرت معانا، never touch، ممنوع ألمس، ممنوع
+
+323
+00:24:14,710 --> 00:24:19,070
+أتذوق، ممنوع أشتم أي مادة كيميائية، ممنوع أخلطهم مع
+
+324
+00:24:19,070 --> 00:24:22,410
+بعض المواد الكيميائية بدون ما الـ .. الـ mess تحكي لي
+
+325
+00:24:22,410 --> 00:24:26,590
+أنا حكيت لك اعملي .. ضيفي الـ .. المادة A على المادة
+
+326
+00:24:26,590 --> 00:24:30,990
+B، و .. و .. و .. و اعملي لهم mix كويس، بتضيفيهم بدون
+
+327
+00:24:30,990 --> 00:24:35,830
+ما أنا أحكي، رجاء، الشخص الكيميائي، أو الـ .. الـ .. الـ
+
+328
+00:24:35,830 --> 00:24:39,090
+.. الإنسان الكيميائي اللي جواتك، اقتليه قبل ما تفوتي
+
+329
+00:24:39,090 --> 00:24:44,500
+المختبر، لإنه هيودينا في داهية. transfer chemicals
+
+330
+00:24:44,500 --> 00:24:47,920
+carefully، طبعا بدي أنقل المواد الكيميائية من مكان
+
+331
+00:24:47,920 --> 00:24:51,540
+لآخر بحذر، بحيث أن أنا يعني ما أضطدم مثلا
+
+332
+00:24:51,540 --> 00:24:55,220
+بزميلتي، و أنا ماشية، اكب عليها، أو ينكب عليا، keep
+
+333
+00:24:55,220 --> 00:24:58,060
+lids on chemical containers، لازم تكون المواد
+
+334
+00:24:58,060 --> 00:25:02,500
+الكيميائية مغطاة، تمام، handle acids and alkalis
+
+335
+00:25:02,500 --> 00:25:07,480
+firmly, upright, and with dry hands، لازم أنا بأستخدم المواد
+
+336
+00:25:07,480 --> 00:25:12,700
+الحمضية و القاعدية بأيدي جافة، و بأيدي لابسة gloves
+
+337
+00:25:12,700 --> 00:25:18,620
+و باستخدام التعليمات اللي حتى أحافظ على نفسي، never
+
+338
+00:25:18,620 --> 00:25:22,300
+pour water into strong acid because of the danger of
+
+339
+00:25:22,300 --> 00:25:25,320
+reaction and splashing، يعني ما بعرفش عندي strong
+
+340
+00:25:25,320 --> 00:25:29,940
+acid، HCl ولا H2SO4، و أنا بدي أخففه و أضيف عليه
+
+341
+00:25:29,940 --> 00:25:35,240
+water مباشرة، لأ ما بنفعش، يعني لإنه هذا ممكن يؤدي إلى
+
+342
+00:25:35,240 --> 00:25:39,100
+إيش، أن يصير splashing، تطير لأ الـ acid و يبتدي
+
+343
+00:25:39,100 --> 00:25:42,280
+يصير حارق، طبعا أنا كده طبعا إيش بدي أعمل لما بدي
+
+344
+00:25:42,280 --> 00:25:46,480
+أخفف الـ acid، بروح بضيف الـ acid للماء، و ليس الماء
+
+345
+00:25:46,480 --> 00:25:50,530
+للـ acid، تتذكر المحاضرة الماضية إيش حكيت لكم؟ حكيت لكم
+
+346
+00:25:50,530 --> 00:25:54,010
+حتى ترتيب الخطوات كويس، عشان هيك لما .. لما هنشتغل
+
+347
+00:25:54,010 --> 00:25:57,630
+مع بعض بالمختبر هنكون ماشيين خطوة خطوة، كلكم يلا
+
+348
+00:25:57,630 --> 00:26:01,010
+اضيفوا المادة، الكل ده، يلا ننتقل للخطوات
+
+349
+00:26:01,010 --> 00:26:05,630
+التانية، و هاكذا، لإنه فعليا أنا لما أجي أحكي للطالب
+
+350
+00:26:05,630 --> 00:26:10,510
+اضيفي، اخففي الـ acid بـ water، فعليا أنك تضيفي الـ
+
+351
+00:26:10,510 --> 00:26:14,670
+water للـ acid شيء مصيبة، لكن أنك أنت مثلا تضيف الـ
+
+352
+00:26:14,670 --> 00:26:19,110
+acid للماء بالتدريج، و على الجدار، و شوي شوي هيك
+
+353
+00:26:19,110 --> 00:26:23,310
+بيكون بطريقة صحيحة، إحنا بنخفف الـ acid و ما .. بنعرض
+
+354
+00:26:23,310 --> 00:26:27,570
+.. بنعرض أنفسنا لخطر أن يتطاير المادة الحمضية القوية
+
+355
+00:26:27,570 --> 00:26:31,350
+هاي الحارقة، و أن نؤذي أنفسنا، أو نؤذي زملائنا، أو
+
+356
+00:26:31,350 --> 00:26:32,430
+المكان المحيط فينا
+
+357
+00:26:35,310 --> 00:26:38,770
+طيب، don't keep acids and alkalis in bottles with ground
+
+358
+00:26:38,770 --> 00:26:42,990
+glass stoppers، طيب الـ .. الـ .. مواقع
+
+359
+00:26:42,990 --> 00:26:47,230
+الأحماض و القواعد هذه بنحطها إحنا في أنابيب زجاجية
+
+360
+00:26:47,230 --> 00:26:49,990
+بس لازم الأنابيب الزجاجية تكون غطيتها plastic
+
+361
+00:26:49,990 --> 00:26:54,430
+ما تكونش غطيتها glass، لأن ممكن الـ glass هذه تصير ..
+
+362
+00:26:54,430 --> 00:26:59,050
+يعني تصير ملتصقة و صعب تفتحها، they .. they may get
+
+363
+00:26:59,050 --> 00:27:03,590
+stuck، ممكن تلتصق، يعني تصير عالقة، مش راضية تفتح
+
+364
+00:27:03,590 --> 00:27:07,500
+النبوبة، consider all chemicals dangerous، طبعا أنت
+
+365
+00:27:07,500 --> 00:27:10,060
+بدك تتعاملي مع كل المواد الكيميائية أنها خطرة
+
+366
+00:27:10,060 --> 00:27:14,340
+عشان تكون بتحمي نفسك، كلها تتعاملي معاها بحذر، و
+
+367
+00:27:14,340 --> 00:27:18,380
+طبعا بنستخدم الـ by bit that are plugged with non
+
+368
+00:27:18,380 --> 00:27:22,260
+absorbent cotton wool with rubber tube attachments
+
+369
+00:27:22,260 --> 00:27:26,260
+بنستخدم الأنواع المخصصة من الـ pipettes عشان ننقل الحجوم
+
+370
+00:27:26,260 --> 00:27:30,260
+اللي فيها، يعني الـ acid و الـ base، هناخدها بالمختبر، و
+
+371
+00:27:30,260 --> 00:27:33,460
+أيضا، every container of chemical is labeled by the
+
+372
+00:27:33,460 --> 00:27:36,820
+manufacturer، حكينا أنه كل مادة كيميائية
+
+373
+00:27:36,820 --> 00:27:40,600
+الزجاجة تبعتها بيكون عليها label جاي من الشركة
+
+374
+00:27:40,600 --> 00:27:45,160
+المصنعة، كل شيء محطوط عليه، مادة كيميائية، اسمها
+
+375
+00:27:45,160 --> 00:27:51,010
+name of chemical، اسم، و عنوان، و الـ emergency
+
+376
+00:27:51,010 --> 00:27:55,210
+phone للمادة المصنعة .. للشركة المصنعة، يعني الـ ..
+
+377
+00:27:55,210 --> 00:27:58,790
+الـ .. الاسم، و العنوان، و التليفون، طوارئ تبع الشركة
+
+378
+00:27:58,790 --> 00:28:04,290
+المصنعة، في حال لو حدث مشكلة، physical and health
+
+379
+00:28:04,290 --> 00:28:08,090
+hazards، يعني هذه المادة ده إيش خطرها عليكي، ممكن
+
+380
+00:28:08,090 --> 00:28:11,730
+تكون موجودة، بيعيقكي، تعليمات، أنها ممكن تكون حارقة،
+
+381
+00:28:11,730 --> 00:28:14,970
+ممكن تكون بتسبب تلف للجلد، ممكن تكون سامة، هذا
+
+382
+00:28:14,970 --> 00:28:17,130
+كله بيكون موجود على الـ bottle من برة، عشان هيك
+
+383
+00:28:17,130 --> 00:28:19,850
+إحنا لازم نقرأ هاي الـ .. الـ label اللي موجودة على
+
+384
+00:28:19,850 --> 00:28:22,900
+الـ bottle، first-hand instructions، بيعطيكي تعليمات
+
+385
+00:28:22,900 --> 00:28:25,560
+أيضا إسعاف أولي، لو هاي المادة انسكبت عليكي، إيش
+
+386
+00:28:25,560 --> 00:28:29,200
+تعملي، أو أنت بالغلط استنشقتيها، أو بالغلط إيش لمست
+
+387
+00:28:29,200 --> 00:28:32,700
+إيديكي، و أيضا proper handling and storage
+
+388
+00:28:32,700 --> 00:28:35,960
+instructions، و كيف أنت تتعاملي معاها، تستخدميها، و
+
+389
+00:28:35,960 --> 00:28:39,520
+كيف تخزنيها، أيضا هذه التعليمات كلها بتكون موجودة
+
+390
+00:28:39,520 --> 00:28:40,680
+على الـ label
+
+391
+00:28:44,270 --> 00:28:48,650
+physical safety، طيب، handle
+
+445
+00:32:59,410 --> 00:33:04,770
+أنا حافظت على حياة الشخص هذا تمام، فبالتالي لازم ال
+
+446
+00:33:04,770 --> 00:33:10,490
+laboratory worker لازم يكون عندهم تعليمات حول
+
+447
+00:33:10,490 --> 00:33:14,270
+الإسعافات الأولية، وأعتقد أنتم عندكم مادة كاملة
+
+448
+00:33:14,270 --> 00:33:18,310
+عن الإسعافات الأولية بتعلمكم في إيش ممكن .. كيف
+
+449
+00:33:18,310 --> 00:33:23,570
+ممكن تتصرفوا في أكثر من موقف لحتى يكون في عندكم
+
+450
+00:33:23,570 --> 00:33:28,660
+خلفية how to first aid myself and my colleagues.
+
+451
+00:33:28,660 --> 00:33:35,640
+طبعًا لازم نعرف إحنا إيش منا نستخدم، إيش منا نعمل، أو
+
+452
+00:33:35,640 --> 00:33:38,780
+إيش منا نتصرف في ال emergency action، في حالات
+
+453
+00:33:38,780 --> 00:33:43,300
+الطوارئ، برضه كمان لو مثلًا عندي مريض أغمى عليه،
+
+454
+00:33:43,300 --> 00:33:49,700
+collapse، مريض اختنق، مريض قدّر فعل عكسية أثناء
+
+455
+00:33:49,700 --> 00:33:54,340
+مثلًا سحب عينة الدم، بخاف هو عادي بتصير هذه كلها
+
+456
+00:33:54,340 --> 00:33:58,500
+برضه كمان لازم أكون بعرف أتصرف فيها المواقف هاي.
+
+457
+00:33:58,500 --> 00:34:04,100
+طبعًا لازم يكون في عندي adequately equipped first
+
+458
+00:34:04,100 --> 00:34:08,180
+aid box، تمام؟ لازم يكون عندي صندوق للإسعافات
+
+459
+00:34:08,180 --> 00:34:11,900
+الأولية، adequately equipped، adequate يعني كافي،
+
+460
+00:34:11,900 --> 00:34:15,300
+equipped، مجهز، يعني مجهز بشكل كافي، يعني موجود فيه
+
+461
+00:34:15,300 --> 00:34:18,830
+كل الأدوات بشكل كافي، فيه ال plasters والـ .. والـ
+
+462
+00:34:18,830 --> 00:34:23,870
+الشاش والكحول والـ iodine والـ .. وفيه كل شيء
+
+463
+00:34:23,870 --> 00:34:29,330
+اللي بيتعلق بالإسعافات الأولية، ماسكات، بلاسترات، كل
+
+464
+00:34:29,330 --> 00:34:34,610
+شيء، تمام؟ ولازم هذا ال box باستمرار، باستمرار أنا
+
+465
+00:34:34,610 --> 00:34:39,810
+أعمله examine، عشان أشيك عليه، إيش ناقص منه، أ .. أ ..
+
+466
+00:34:39,810 --> 00:34:44,590
+أعبيه مرّة ثانية، وأخليه جاهز، وأخليه متوفر فيه كل
+
+467
+00:34:44,590 --> 00:34:48,810
+الأدوات اللي بتكون موجودة عندي، طبعًا لازم يكون هذا
+
+468
+00:34:48,810 --> 00:34:52,010
+ال box مصنوع من ال metal أو ال plastic علشان ما
+
+469
+00:34:52,010 --> 00:35:01,470
+يتكسر، وبرضه كمان عشان .. ولازم يكون نظيف ومغلق،
+
+470
+00:35:01,470 --> 00:35:06,610
+وموجود في مكان الكل بيعرف يصله لحتى نسهّل من عملية
+
+471
+00:35:06,720 --> 00:35:10,280
+ال first aiding اللي هي الإسعافات الأولية، طبعًا إيش
+
+472
+00:35:10,280 --> 00:35:15,480
+مكونات الـ .. الإسعافات .. صندوق الإسعافات الأولية؟
+
+473
+00:35:15,480 --> 00:35:19,780
+هي بيكون لازم يكون فيه sterile dressing، يعني شاش
+
+474
+00:35:19,780 --> 00:35:23,970
+معقّم، عشان نغطي الجروح، to cover wounds، absorbent
+
+475
+00:35:23,970 --> 00:35:27,750
+cotton، .. cotton، عشان يمتص السوائل أو المواد
+
+476
+00:35:27,750 --> 00:35:31,430
+المعقّمة أو أي شيء، لازم يكون في عندي roll
+
+477
+00:35:31,430 --> 00:35:35,550
+bandage، اللي هي اللصقة اللي عاملة زي رولات، أو حتى
+
+478
+00:35:35,550 --> 00:35:38,890
+اللي واخدة شكل المثلّثات، زي اللي بنحط على العين، أو
+
+479
+00:35:38,890 --> 00:35:43,310
+شيء، sterile eye pad، لازم برضه كمان قطن خاص بالعين
+
+480
+00:35:43,310 --> 00:35:51,670
+معقّم، رول أدهيس، الشاش اللاصق أو الشريط اللاصق، سيزورز،
+
+481
+00:35:51,670 --> 00:35:54,890
+مقصّات، لازم يكون موجود فيه Sodium Bicarbonate
+
+482
+00:35:54,890 --> 00:35:58,470
+Powder، و Boric Acid Powder، و 5% Acetic Acid، و
+
+483
+00:35:58,470 --> 00:36:01,810
+Magnesium Hydroxide Suspension، طبعًا هذه المواد الأربعة
+
+484
+00:36:01,810 --> 00:36:05,590
+المحاليل أو المواد الكيميائية، راح نعرف استخدامها في
+
+485
+00:36:05,590 --> 00:36:06,830
+ال slides الجاية.
+
+486
+00:36:09,620 --> 00:36:15,030
+تمام، إيش بيحدث لو صار في عندي cut، جرح، و bleeding، إيش
+
+487
+00:36:15,030 --> 00:36:18,330
+بيصير؟ أول شيء بدي أغسل المكان بالماء والصابون،
+
+488
+00:36:18,330 --> 00:36:21,670
+تمام؟ بعدين حكينا apply pressure with a piece of
+
+489
+00:36:21,670 --> 00:36:26,970
+cotton، بدي أسكّر الجرح بقطعة قطن، تمام؟ أعقّم
+
+490
+00:36:26,970 --> 00:36:31,910
+المكان وألفّه، أعقّمه ب iodine وألفّه، تمام؟ cover
+
+491
+00:36:31,910 --> 00:36:36,070
+.. هي هنا disinfect، بعقّم وأ cover، بلفّه، تمام؟
+
+492
+00:36:36,070 --> 00:36:43,710
+بالشاش، إذا كان الجرح، طبعًا، يعني أنا انجرحت بمادّة
+
+493
+00:36:43,710 --> 00:36:48,390
+ملوّثة، إيش أعمل؟ أولًا بدي أغسل إيدي بالماء
+
+494
+00:36:48,390 --> 00:36:52,090
+والصابون، بعدين بدي أعمل encouraging bleeding of
+
+495
+00:36:52,090 --> 00:36:55,450
+two minutes، يعني أنا بدي أعصر إيدي وأخليها تنزف
+
+496
+00:36:55,450 --> 00:36:59,770
+لمدّة two minutes، طبعًا، ليش؟ لأن الدم هذا اللي
+
+497
+00:36:59,770 --> 00:37:03,680
+بيطلع من الجروح بياخد ال contamination، المواد
+
+498
+00:37:03,680 --> 00:37:07,540
+الملوّثة اللي .. اللي دخلت جوا الجرح وبيطلّعها لبره،
+
+499
+00:37:07,540 --> 00:37:11,200
+زي كأني أنا قاعدة بعمل washing بال bleeding تبعي،
+
+500
+00:37:11,200 --> 00:37:16,600
+تمام؟ بأغسل ثاني بالماء والصابون، وبَرجع بأطبّق نفس
+
+501
+00:37:16,600 --> 00:37:20,440
+الأدوات، وإذا كان الجرح عميق، ممكن أنا أسأل .. أو
+
+502
+00:37:20,440 --> 00:37:26,080
+يعني الدكتور يجي يعمل قطب، غرز طبيّة، أو .. أو ما
+
+503
+00:37:26,080 --> 00:37:26,500
+شابه.
+
+504
+00:37:29,320 --> 00:37:35,180
+طبعًا، لو كان في عندي حروق باستخدام الحرارة، لو حدث
+
+505
+00:37:35,180 --> 00:37:37,960
+حرق باستخدام الحرارة، أنا لمست شيء ساخن بدون ما
+
+506
+00:37:37,960 --> 00:37:42,040
+أنتبه، إيش بدي أعمل؟ على طول immerse the burnt
+
+507
+00:37:42,040 --> 00:37:46,180
+area، المكان اللي صار له احتراق، على طول بغمره تحت
+
+508
+00:37:46,180 --> 00:37:52,980
+الماء البارد، وطبعًا or apply soaked bed with cold
+
+509
+00:37:52,980 --> 00:37:57,840
+water، يعني يا إما بغمر إيدي تحت ماء بارد جاري لمدة
+
+510
+00:37:57,840 --> 00:38:02,680
+عشر دقائق، أو بحط قطنة فيها ماء بارد على المكان زي
+
+511
+00:38:02,680 --> 00:38:07,200
+كمادّة، يعني لمدة عشر دقائق، و cover with dry
+
+512
+00:38:07,200 --> 00:38:11,200
+dressing، وبعدين بغطي المكان بشاش، إذا كان الجرح
+
+513
+00:38:11,200 --> 00:38:16,420
+severe، أو الحرق severe، يعني طويل، أكيد بوجه الشخص
+
+514
+00:38:16,420 --> 00:38:20,260
+هذا اللي اتعرّض للحرق، لل medical treatment، بحاوله
+
+515
+00:38:20,260 --> 00:38:27,000
+على العيادة أو للطبيب، بحيث إنه يشوف علاج للجرح أو
+
+516
+00:38:27,000 --> 00:38:32,520
+للحرق، تمام؟ ال chemical burn of the skin، لو أنا
+
+517
+00:38:32,520 --> 00:38:37,320
+احترقت بفعل مادة كيميائية وليس بفعل الحرارة، إيش
+
+518
+00:38:37,320 --> 00:38:41,300
+بدي أعمل؟ أول حاجة بدي أغسل المكان بكمية كبيرة من
+
+519
+00:38:41,300 --> 00:38:45,260
+الماء، بعدين بدي أعمل ال neutralizing للمادة
+
+520
+00:38:45,260 --> 00:38:48,920
+الكيميائية، يعني أنا لو احترقت ب acid بدي أعمل ال
+
+521
+00:38:48,920 --> 00:38:52,940
+neutralization، يعني بدي أضيف على مكان الحرق sodium
+
+522
+00:38:52,940 --> 00:38:55,920
+bicarbonate، عشان ال sodium bicarbonate هي قاعدة
+
+523
+00:38:56,390 --> 00:38:59,710
+هتعادل ال acid، وبالتالي تخفف من آثار ال damage
+
+524
+00:38:59,710 --> 00:39:05,110
+اللي ممكن يسببها لي، وإذا أنا احترقت بمادة alkaline
+
+525
+00:39:05,110 --> 00:39:08,630
+بدي أضيف، أعمل neutralization ب boric acid powder
+
+526
+00:39:08,630 --> 00:39:14,850
+عشان هيك حكينا إن صندوق الإسعافات
+
+527
+00:39:14,850 --> 00:39:17,990
+الأولية لازم يكون فيه sodium bicarbonate و boric
+
+528
+00:39:17,990 --> 00:39:26,320
+acid، تمام؟ طيب، لو المادة الكيميائية فاتت للعين، إيش
+
+529
+00:39:26,320 --> 00:39:31,120
+بدي أعمل؟ حكينا، قلنا إنه بدي أغسلها بالماء الجاري
+
+530
+00:39:31,120 --> 00:39:35,060
+لمدّة ربع ساعة، وأعمل ال neutralize للمادة
+
+531
+00:39:35,060 --> 00:39:38,520
+الكيميائية هذه، طبعًا إذا كانت المادة الكيميائية acid
+
+532
+00:39:38,520 --> 00:39:42,700
+اللي فاتت للعين، بدي أستخدم عشان أعادلها وأقلّل من
+
+533
+00:39:42,700 --> 00:39:47,280
+تأثيرها، بدي أستخدم 5% Sodium Bicarbonate، إذا كانت
+
+534
+00:39:47,280 --> 00:39:52,370
+alkaline، بدي أستخدم 5% Acetic Acid، immediately seek
+
+535
+00:39:52,370 --> 00:39:56,210
+medical attention، على طول بأعلم الطبيب أو العيادة إنّه
+
+536
+00:39:56,210 --> 00:40:00,270
+صار في عندنا حادث، شخص اتعرّض، يعني اتقشّف في عينه
+
+537
+00:40:00,270 --> 00:40:03,430
+مادة كيميائية، وإحنا عملنا له ال first aid، وعليكم
+
+538
+00:40:03,430 --> 00:40:04,050
+يتكمّل.
+
+539
+00:40:06,680 --> 00:40:11,460
+swallowing an acid or base، يعني ابتلاع مادة فيها
+
+540
+00:40:11,460 --> 00:40:15,740
+حمض أو قاعدة، إيش بدنا نعمل؟ أول حاجة immediately
+
+541
+00:40:15,740 --> 00:40:19,060
+rinse the mouth with water، يعني أول حاجة بدي أغمر
+
+542
+00:40:19,060 --> 00:40:23,810
+الماء، أخليه يضمّض، يعني أكثر من مرّة بالماء، وبعدين
+
+543
+00:40:23,810 --> 00:40:28,970
+بروح، إذا كان هو ابتلع acid، بدي أعمله neutralization
+
+544
+00:40:28,970 --> 00:40:33,710
+فيه بـ يعني في 8% Magnesium Hydroxide، عشان هيك
+
+545
+00:40:33,710 --> 00:40:38,510
+ال Magnesium Hydroxide من أحد مكونات ال first aid
+
+546
+00:40:38,510 --> 00:40:43,950
+box، تمام؟ أو ممكن أخليه يشرب حليب، milk of magnesia،
+
+547
+00:40:43,950 --> 00:40:48,350
+تمام؟ عشان يعادل ال acid اللي هو ابتلعه، إذا كان
+
+548
+00:40:48,350 --> 00:40:52,910
+الشخص ابتلع alkaline، معناها لازم يعادلها بـ
+
+549
+00:40:52,910 --> 00:40:57,710
+acid، بأعطيه lemon juice أو 5% acetic acid، drink
+
+550
+00:40:57,710 --> 00:41:02,250
+three or four cups of water، لازم يشرب مية كتير،
+
+551
+00:41:02,250 --> 00:41:05,330
+عشان يعمل dilution لهذا ال acid وال base اللي ..
+
+552
+00:41:05,330 --> 00:41:09,210
+اللي ابتلعه، و seek medical attention، بوجهه للرعاية
+
+553
+00:41:09,210 --> 00:41:13,070
+الصحية، وبأعلم العيادة أو الدكتور إنّ هو صار في
+
+554
+00:41:13,070 --> 00:41:18,310
+عندنا حادث، لحتى يحطّوه تحت المراقبة، لما الشخص يبتلع
+
+555
+00:41:18,310 --> 00:41:21,770
+acid أو alkaline، don't encourage vomiting،
+
+556
+00:41:21,770 --> 00:41:27,010
+ما بنخليهوش يستفرغ، ليش؟ لأنّه هالجيت إحنا النسيج تبع
+
+557
+00:41:27,010 --> 00:41:32,150
+البلعوم والحنجرة والمريء وحتى المعدة، هذا عبارة
+
+558
+00:41:32,150 --> 00:41:37,410
+.. النسيج هذا احترق أول مرّة لما .. لما لمس ال acid
+
+559
+00:41:37,410 --> 00:41:40,930
+أو ال alkaline اللي إحنا ابتلعناه، تمام؟ لما نيجي
+
+560
+00:41:40,930 --> 00:41:45,100
+نعمل vomiting، معناها هيبدا يحترق مرّة ثانية، يعني أنا
+
+561
+00:41:45,100 --> 00:41:48,080
+هيك بزود ال damage، كمية ال tissue اللي بيصير لها
+
+562
+00:41:48,080 --> 00:41:51,460
+damage بسبب تعرضها لل acid وال alkaline بتكون
+
+563
+00:41:51,460 --> 00:41:55,220
+كتير كبيرة، إذا أنا بَخَلّي الشخص ما بفيده، إذا هخليه
+
+564
+00:41:55,220 --> 00:42:00,460
+يستفرغ، فالأفضل إنّه أنا أخليه يغسل فمه، نعادل المادة
+
+565
+00:42:00,460 --> 00:42:05,440
+اللي أخدها، أخليه يشرب مية لحتى يعمل dilution، ونحطّه
+
+566
+00:42:05,440 --> 00:42:06,220
+تحت المراقبة.
+
+567
+00:42:08,730 --> 00:42:12,730
+إذا ابتلع الشخص أي مادة سامة أخرى، swallowing of
+
+568
+00:42:12,730 --> 00:42:18,050
+other poisonous chemical، طبعًا لازم يغسل الـ .. فمه
+
+569
+00:42:18,050 --> 00:42:22,230
+بالماء جيدًا، وطبعًا تبع الـ .. المادة الكيميائية هذه
+
+570
+00:42:22,230 --> 00:42:25,030
+بستخدم ال antidote تبعها، إيش يعني ال antidote؟ يعني
+
+571
+00:42:25,030 --> 00:42:29,550
+literally neutralizing agent تبعها، يعني إذا كانت
+
+572
+00:42:29,550 --> 00:42:33,830
+acid، بنشوف هو إيش ممكن .. هاي هنا نرجع ثاني، إيش
+
+573
+00:42:33,830 --> 00:42:38,560
+ابتلع acid؟ إيش بنخلّق .. بنعمل لهم ال neutralization
+
+574
+00:42:38,560 --> 00:42:44,020
+بيه، Magnesium Hydroxide أو magnesia؟ إذا ابتلع مادة
+
+575
+00:42:44,020 --> 00:42:48,480
+alkaline، بنعطيه lemon juice أو 5% acetic acid، بس
+
+576
+00:42:48,480 --> 00:42:52,000
+هنا الفرق إيش؟ إن المادة سامة، فإيش بدي أنا أخد
+
+577
+00:42:52,000 --> 00:42:56,760
+إجراءات ثانية؟ على طول بدي أودّيه على الـ .. الـ
+
+578
+00:42:56,760 --> 00:42:59,660
+chemical .. على ال physical، أو sorry، يعني الـ
+
+579
+00:42:59,660 --> 00:43:05,040
+physician، على الدكتور، لحتى يعني يعطيه علاج، أو
+
+580
+00:43:05,040 --> 00:43:09,920
+يعطيه، يعمل له غسيل المعدة، أو أي إجراء لحتى يخلّصه
+
+581
+00:43:09,920 --> 00:43:13,760
+من أثر المادة السامة هاي اللي ابتلعها، Swallowing
+
+582
+00:43:13,760 --> 00:43:18,200
+of infected material، إذا .. إذا ابتلع محلول فيه
+
+583
+00:43:18,200 --> 00:43:21,260
+بكتيريا أو فطريات أو أي مادة معديّة، infection، ممكن
+
+584
+00:43:21,260 --> 00:43:25,780
+تعمل له infection، على طول بروح بـ .. بوجهه لطبيب هو
+
+585
+00:43:25,780 --> 00:43:32,280
+اللي بيتعامل معاه، تمام؟ إذا الشخص اتعرّض ل electric
+
+586
+00:43:32,280 --> 00:43:37,340
+shock، يعني صدمة كهربائية، بدي أـ .. أول شيء بدي أطفئ
+
+587
+00:43:37,340 --> 00:43:41,620
+الكهرباء from the mains، من المصدر الرئيسي، بدي أنزل
+
+588
+00:43:41,620 --> 00:43:46,020
+القاطع، تمام؟ وإذا الشخص صار له collapse، يعني أغمى
+
+589
+00:43:46,020 --> 00:43:51,150
+عليه أو فقد وعيه أو غاب، على طول بدي أجيب .. بدي
+
+590
+00:43:51,150 --> 00:43:56,070
+أسأل الطبيب أو ممرض أو أي حد ييجي يسعفه، وأتأكد
+
+591
+00:43:56,070 --> 00:44:02,290
+إنّه مجرد تنفّس عنده مفتوح، أرفع رجليه لفوق، أحاول إنّه
+
+592
+00:44:02,290 --> 00:44:06,390
+أخلّ .. يعني أرفع رأسه .. يعني أنزل رأسه لتحت و رقبته
+
+593
+00:44:06,390 --> 00:44:10,500
+هيك، أخليها .. عالية شوية، علشان أتأكّد إنّه مجرد
+
+594
+00:44:10,500 --> 00:44:18,220
+تنفّس عنده فاتح، وطبعًا أحاول إنّه إذا كان الوضع صعب
+
+595
+00:44:18,220 --> 00:44:23,660
+على الآخر، إنّه نعطيه إنعاش قلب ورئتين. طيب، كيف أنا
+
+596
+00:44:23,660 --> 00:44:26,320
+بدي أحافظ على الـ .. نظافة المختبر، laboratory
+
+597
+00:44:26,320 --> 00:44:30,640
+hygiene، ال hygiene يعني نظافة، never eat, never
+
+598
+00:44:30,640 --> 00:44:33,720
+drink, never smoke، يعني لا تأكلوا ولا تشربوا ولا
+
+599
+00:44:33,720 --> 00:44:36,820
+تدخّنوا داخل المختبرات، طبعًا يعني الدنيا مش مستاهلة
+
+600
+00:44:36,820 --> 00:44:42,720
+نجي ندخّن علشان حاجات رايحة، never apply cosmetics،
+
+601
+00:44:42,720 --> 00:44:47,800
+ما تحطّوش ما .. ما .. ماكياج، تجميل، إحنا حلوين ربانيًّا،
+
+602
+00:44:47,800 --> 00:44:51,800
+never touch your face or mouth or eyes، ما تحاولوش إنّ
+
+603
+00:44:51,800 --> 00:44:54,900
+أنا ألمس .. أضلّ ألمس الوجه والأيدين والـ eyes،
+
+604
+00:44:54,900 --> 00:44:58,700
+بتلاحظوا إحنا هنا لقينا ما .. مع علامات الـ .. أو
+
+605
+00:44:58,700 --> 00:45:02,680
+sorry، مع تحضيرات للوقاية من virus corona، بنضلّ نقول إنّه يا جماعة الخير اغسلوا أيديكم منيّح،
+
+606
+00:45:02,680 --> 00:45:05,780
+ما تلمسوش وجهكم، أنفكم، عيونكم، هذه برضه إجراءات
+
+607
+00:45:05,780 --> 00:45:09,600
+وقائية لكتير أمراض، فبالتالي، don't touch your face،
+
+608
+00:45:09,600 --> 00:45:13,160
+mouth، eyes، ملاحظ إنّه الطالبة وهي لابسة ال gloves
+
+609
+00:45:13,220 --> 00:45:17,280
+بتنصدم هيك وبتتفاجأ هي وزملتها، فعلى طول بتروح
+
+610
+00:45:17,280 --> 00:45:21,180
+تشحطّ إيدها على وجهها أو على ثمّها، وهذه طبعًا علامات
+
+611
+00:45:21,180 --> 00:45:24,720
+الدهشة، فرجاء لا تندهشوا، علشان إنّه ما تحافظوا على
+
+612
+00:45:24,720 --> 00:45:30,340
+ال hygiene تبعكم، never suck pens or chew pencil،
+
+667
+00:49:32,560 --> 00:49:37,980
+ممكن نعرض أنفسنا لخطر، حكينا هذه الـ slide، شرحناها
+
+668
+00:49:37,980 --> 00:49:43,380
+قولنا إنه مش لازم أن أطلق حس الدعابة داخل المختبر
+
+669
+00:49:43,380 --> 00:49:48,340
+وأعمل مجاملات لزملائي عشان ما أعرض نفسي أو أعرضهم 
+
+670
+00:49:48,340 --> 00:49:52,400
+للأذى، وبرضه كمان في عندنا مخازن بتكون مخزن عليها
+
+671
+00:49:52,400 --> 00:49:56,590
+المواد الكيميائية، ممنوع دخولها إلا بإذن، وبرضه
+
+672
+00:49:56,590 --> 00:49:59,770
+كمان ممنوع أخذ أي مادة كيميائية منها برضه إلا
+
+673
+00:49:59,770 --> 00:50:06,790
+بإذن، ولمس أو استخدام أي مادة كيميائية إلا إذا
+
+674
+00:50:06,790 --> 00:50:09,370
+أنا أعطيتك تعليمات فيها، وغالبا إنه بيكون اللي
+
+675
+00:50:09,370 --> 00:50:14,190
+متعامل مع المخزن أنا والفني بالمختبر، حتى كمان أنا
+
+676
+00:50:14,190 --> 00:50:17,810
+بيكون الـ access تبعي على هذا المخزن قليل، يعني
+
+677
+00:50:17,810 --> 00:50:21,690
+دخولي للمخزن بيكون قليل جداً، غالباً بيكون في عندنا موظف
+
+678
+00:50:21,690 --> 00:50:25,030
+فني مختبر، أنا دائماً بكتب له ورقة بأسماء المواد اللي
+
+679
+00:50:25,030 --> 00:50:28,270
+بدي إياها، فهو بيطلع لي إياها من المخزن، وهيك أنا بكون حميت
+
+680
+00:50:28,270 --> 00:50:31,390
+نفسي، فإنه كمان برضه الطالبات مش لازم يغامروا
+
+681
+00:50:31,390 --> 00:50:36,910
+يدخلوا للمخزن ويعرضوا أنفسهن للخطر، إذن بدنا نتذكر
+
+682
+00:50:36,910 --> 00:50:40,510
+إنه لازم stay at your work station، لازم نضل في
+
+683
+00:50:40,510 --> 00:50:44,170
+مكان عملنا طول الوقت، وعندنا شغل maintain أكلين و...و
+
+684
+00:50:44,170 --> 00:50:48,910
+a clean work area، لازم يكون في عندي مكان عمل نظيف
+
+685
+00:50:48,910 --> 00:50:52,810
+أحافظ عليه نظيفاً، read and follow all directions، كل
+
+686
+00:50:52,810 --> 00:51:00,030
+التعليمات أقرأها جيداً وأتبعها، أي spill، انسكاب، حادث
+
+687
+00:51:00,030 --> 00:51:03,790
+أو injury، إصابة، أتعرض لها أو يتعرض لها زملائي، لازم
+
+688
+00:51:03,790 --> 00:51:09,830
+تصير reported، يعني تكتب وتسجل، ولازم أن أعمل
+
+689
+00:51:09,830 --> 00:51:14,090
+cleaning، تنظيف، أو أرفع كل الـ equipment بعد ما أخلص
+
+690
+00:51:14,090 --> 00:51:18,190
+من استخدامها، وأتخلص من الـ waste بالشكل المناسب، و
+
+691
+00:51:18,190 --> 00:51:21,630
+هيك بنكون خلصنا علامات السلامة والأمان اللي داخل
+
+692
+00:51:21,630 --> 00:51:27,450
+المختبر، وخلصنا محاضرتنا لليوم، طبعاً حاولوا أنكم
+
+693
+00:51:27,450 --> 00:51:31,370
+تسمعوها مرة ومرتين وثلاث، ومش غلط أنكم تعملوا
+
+694
+00:51:31,370 --> 00:51:35,830
+تلخيص بأهم المعلومات اللي داخلة داخل الـ...اللي
+
+695
+00:51:35,830 --> 00:51:40,170
+يعني اللي داخلة داخل الـ chapter هذا، وحتى أنه يسهل
+
+696
+00:51:40,170 --> 00:51:45,990
+رجوعكم إليها، ويسهل دراستها، بشدّد يعني أو ب...ب...
+
+697
+00:51:45,990 --> 00:51:52,830
+بشدّد عليكم، أو يعني أنوّه...أنوّه...الكلمات العربية
+
+698
+00:51:52,830 --> 00:51:57,470
+أحياناً بتضيع مني، أنه يعني المواد الكيميائية هذه
+
+699
+00:51:57,470 --> 00:52:00,970
+اللي أنا ببدأ أستخدمها للـ...للـ neutralization مهمة
+
+700
+00:52:00,970 --> 00:52:04,130
+يعني مثلاً في حالة الحرق بـ acid، إيش أستخدم له
+
+701
+00:52:04,130 --> 00:52:07,130
+neutralization؟ في حالة الـ alkaline، إيش أستخدم؟ في
+
+702
+00:52:07,130 --> 00:52:10,210
+حالة الإصابة؟ في حالة المادة اللي بت...بتفوت جوا
+
+703
+00:52:10,210 --> 00:52:13,050
+العين؟ إذا كانت acid أو base، إيش أستخدم لها؟ الـ
+
+704
+00:52:13,050 --> 00:52:16,570
+antidote تبعها أو الـ neutralization تبعها، تمام؟
+
+705
+00:52:16,570 --> 00:52:19,770
+كلمة antidote أو neutralizing agent نفس المعنى
+
+706
+00:52:20,150 --> 00:52:23,830
+فالمهم هذول، احفظوهم منيح، لو أنكم لخصتمهم على
+
+707
+00:52:23,830 --> 00:52:28,170
+ورقة واستخدمتوهم، يعني راجعتوهم خلال كل دراستكم أو
+
+708
+00:52:28,170 --> 00:52:32,120
+كل ما يكون عليك الـ quiz أو شيء، ترجع إليهم، مش هتغلطوا. هيك
+
+709
+00:52:32,120 --> 00:52:36,040
+بنكون خلصنا محاضرتنا لليوم، يعطيكم ألف عافية و
+
+710
+00:52:36,040 --> 00:52:39,340
+شكراً لكم، أي استفسار، أي سؤال بالمحاضرة هذه و
+
+711
+00:52:39,340 --> 00:52:42,600
+المحاضرة السابقة، إن شاء الله نلقاكم يوم السبت
+
+712
+00:52:42,600 --> 00:52:47,100
+الساعة السادسة على الـ zoom، لحتى نلتقي بكم أو نستقبل
+
+713
+00:52:47,100 --> 00:52:52,100
+منكم الأسئلة ونأخذ إجابات عليها، أو يصير حتى نقاش
+
+714
+00:52:52,100 --> 00:52:56,260
+حولها، ممكن أضرب لكم أمثلة لحتى أقرب لكم الفهم، ممكن
+
+715
+00:52:56,260 --> 00:52:58,960
+البنت مش فاهمة الـ slide، أرجع أشرح لها إياها، نفتح الـ
+
+716
+00:52:58,960 --> 00:53:03,480
+slide ونرجع نقرأها ونشرحها، ممكن أنا يكون عندي
+
+717
+00:53:03,480 --> 00:53:08,400
+أسئلة، أو أنتم يكون عندكم أسئلة، فالساعة اللي بناخذ
+
+718
+00:53:08,400 --> 00:53:12,180
+فيها مناقشة على الـ zoom مهمة جداً يا بنات، ورجاءً ما
+
+719
+00:53:12,180 --> 00:53:15,520
+تفرّطوا فيها، وزي ما حكيت لكم إذا حسيت ما فيها الـ
+
+720
+00:53:15,520 --> 00:53:20,240
+design، رح يكون عليها حضور وغياب، هيك أنا بكون خلصت
+
+721
+00:53:20,240 --> 00:53:23,100
+وموفقين بإذن الله، شكراً لكم
diff --git a/PL9fwy3NUQKwY9jl8xw-bWkVZkWcGGyspd/fqKeQtgENUo_postprocess.srt b/PL9fwy3NUQKwY9jl8xw-bWkVZkWcGGyspd/fqKeQtgENUo_postprocess.srt
new file mode 100644
index 0000000000000000000000000000000000000000..102f12ee58b239a2710bead58989871d4f4e8c57
--- /dev/null
+++ b/PL9fwy3NUQKwY9jl8xw-bWkVZkWcGGyspd/fqKeQtgENUo_postprocess.srt
@@ -0,0 +1,2884 @@
+1
+00:00:10,530 --> 00:00:14,590
+السلام عليكم ورحمة الله وبركاته طلبات العزيزات
+
+2
+00:00:14,590 --> 00:00:19,590
+اليوم ان شاء الله رح نكمل موضوعنا المفروض يعني هو
+
+3
+00:00:19,590 --> 00:00:26,370
+يعني يندرج تحت العملي عن ال laboratory safety عن
+
+4
+00:00:26,370 --> 00:00:32,030
+قواعد السلام والأمانةالصراحة المحاضرة اليوم فيها
+
+5
+00:00:32,030 --> 00:00:38,150
+يعني ملاحظات كتير فيها كلام كتير ولكن ال .. الاشي
+
+6
+00:00:38,150 --> 00:00:45,330
+مفهوم يعني و هو ممكن انتوا يعني تحسي حالك زهجتي من
+
+7
+00:00:45,330 --> 00:00:49,030
+الكلام بس في النهاية انت يعني انا .. انا بتخيل انه
+
+8
+00:00:49,030 --> 00:00:54,530
+انك تسمعي لمرة واحدة او مرتينراح يرسخ يعني هو مش
+
+9
+00:00:54,530 --> 00:00:58,690
+صعب بس إنما هو كلام و حتى هيكون مكرر يعني في جزئية
+
+10
+00:00:58,690 --> 00:01:04,330
+منيحة يعني أنه شرحناها أو غطيناها خلال المحاضرة
+
+11
+00:01:04,330 --> 00:01:09,130
+النظرية السابقة فالكلام يعني هيكون في جزء جديد
+
+12
+00:01:09,130 --> 00:01:13,210
+عليكم في جزء اسمعته قبل هيك فأتمنى إن شاء الله
+
+13
+00:01:13,210 --> 00:01:16,950
+يعني تكون كل الأمور تمام و كلها باضحةطبعا احنا
+
+14
+00:01:16,950 --> 00:01:21,330
+حكينا في ال .. في المختبر طبعا أنا ليش بحافظة على
+
+15
+00:01:21,330 --> 00:01:25,710
+قواعد السلامة و الأمان علشان ال .. ال safe working
+
+16
+00:01:25,710 --> 00:01:30,070
+الشغل الأمن بيحمي مين؟ بيحمي نفسي و ال other lab
+
+17
+00:01:30,070 --> 00:01:34,430
+workers زملائي و ال cleaners ال .. الناس عمال
+
+18
+00:01:34,430 --> 00:01:37,870
+النظافة اللي بيشرفوا على نظافة المكان ال visitors
+
+19
+00:01:37,870 --> 00:01:42,850
+زوار المكان و يعني المرضى اللي بيرتعدوا المكانو
+
+20
+00:01:42,850 --> 00:01:46,610
+أيضا الشغل تبعي طالما أنا إذا كنت بشتغل شغل safe
+
+21
+00:01:46,610 --> 00:01:51,330
+شغل آمن إذا هيكون شغلي صحيح تمام و هحصل على نتائج
+
+22
+00:01:51,330 --> 00:01:56,890
+صحيحة تفيد في ال diagnosis حكينا
+
+23
+00:01:56,890 --> 00:02:00,570
+.. هذا .. هذا كلام مععد حكينا قبل هيك أنه لازم
+
+24
+00:02:00,570 --> 00:02:04,930
+أحافظ على ارتداء ال lab coat تمام و لازم ال long
+
+25
+00:02:04,930 --> 00:02:09,250
+hair يكون مرفوع الشعر اللي طويل .. sorry
+
+26
+00:02:12,690 --> 00:02:18,330
+الشعر لازم يكون مرفوع و طبعا ما نلبسش ملابس واسعة
+
+27
+00:02:18,330 --> 00:02:22,230
+و لازم ال shoes يكون closed مش يكون مفتوح ال
+
+28
+00:02:22,230 --> 00:02:26,750
+sandals و الأشياء المفتوحة هاي نروح .. نحاول أنه
+
+29
+00:02:26,750 --> 00:02:30,840
+ما ناخدهاتمام؟ و بالنسبة كمان ايضا لازم ارتدي ل
+
+30
+00:02:30,840 --> 00:02:33,260
+gloves، ل gloves انه انا في عندي نوعين من ال
+
+31
+00:02:33,260 --> 00:02:36,520
+gloves، في ال gloves اللي هي مصنوعة من ال latex
+
+32
+00:02:36,520 --> 00:02:40,540
+اللي زي ال nylon هاد، هاد اللي احنا بنستخدمه للشغل
+
+33
+00:02:40,540 --> 00:02:44,720
+طبعنا، طبعا في منه أنواع و في منه درجات حتى في
+
+34
+00:02:44,720 --> 00:02:48,960
+نسبة ال protection يعني في بيحمي أكتر في بيحمي أقل
+
+35
+00:02:49,170 --> 00:02:55,070
+فرح ناخدهم طبعا بالتفصيل لقدام شوية و أيضا في ال
+
+36
+00:02:55,070 --> 00:02:59,890
+gloves السميك اللي مصنوع من النسيج أو القماش اللي
+
+37
+00:02:59,890 --> 00:03:07,210
+هو للتعامل مع الأشياء الساخنةطبعا في قاعدة بتحكيلك
+
+38
+00:03:07,210 --> 00:03:09,610
+remove your gloves before using an instrument,
+
+39
+00:03:09,830 --> 00:03:12,490
+telephone and leaving the laboratory طبعا قبل أن
+
+40
+00:03:12,490 --> 00:03:16,830
+أستخدم أي جهاز و أنا بتعامل مع العين أو المحاليل و
+
+41
+00:03:16,830 --> 00:03:19,410
+الأدوات لازم أكون لابسة gloves لما أجد أستخدم جهاز
+
+42
+00:03:19,410 --> 00:03:22,510
+أو حتى ال telephone تبعي أو ألمس أغراضي أو أغادر
+
+43
+00:03:22,510 --> 00:03:25,890
+من المختبر لازم أكون تخلص من ال gloves و أتلفتها
+
+44
+00:03:25,890 --> 00:03:30,420
+علشان ما أنشر ال contaminationeverywhere طبعا لو
+
+45
+00:03:30,420 --> 00:03:34,100
+انسكب اي مادة كيميائية على الجلب لازم انا اغسلها
+
+46
+00:03:34,100 --> 00:03:38,420
+بالماء لمدة ربع ساعة لازم اغسل يديا قبل و بعد
+
+47
+00:03:38,420 --> 00:03:44,140
+المختبر و ايضا ال .. ال .. ال .. هو انا مش عارفة
+
+48
+00:03:44,140 --> 00:03:51,710
+من اللي عامله عرض فهو العرض يعنيغريب، طيب و بحكيلك
+
+49
+00:03:51,710 --> 00:03:55,450
+هنا handle a glassware sharp tool and heated
+
+50
+00:03:55,450 --> 00:04:00,030
+containers carefully، اتعاملي مع المواد الزجاجية،
+
+51
+00:04:00,030 --> 00:04:04,690
+الأدوات الحادة و الأدوات الساخنة بحذر
+
+52
+00:04:09,980 --> 00:04:13,420
+بنتعامل مع .. بنستخدم ال safety goggles النضارات
+
+53
+00:04:13,420 --> 00:04:17,520
+لو انا بستخدم مادة كيميائية لها ال .. يعني لها
+
+54
+00:04:17,520 --> 00:04:25,220
+بمتطاير او رذاذ متطاير او حتى يعني depress اللي هي
+
+55
+00:04:25,220 --> 00:04:30,040
+يعني بقايا متطايرة ممكن اتلامسها عيوني انا بتخلص
+
+56
+00:04:30,040 --> 00:04:37,940
+منها اذا كان طبعا اي حد بيرتدي عدساتلاصقة لازم
+
+57
+00:04:37,940 --> 00:04:43,120
+يخبر الدكتور و أيضا يعني يخبر المدرس اللي موجود في
+
+58
+00:04:43,120 --> 00:04:48,560
+المختبر و أيضا في حالة ال .. ال emergency لو يعني
+
+59
+00:04:48,560 --> 00:04:51,860
+في حالة الطوارئ لو أي إشي أي chemical دخلت إلى
+
+60
+00:04:51,860 --> 00:04:55,240
+العين لازم أنا أخسلها من خلال ال washer تبع العين
+
+61
+00:04:55,240 --> 00:04:58,380
+لمدة ربع ساعة و احنا حكينا عن ال washer هذه
+
+62
+00:04:58,380 --> 00:05:01,880
+المحاضرة الماضيةطبعا إيش الأخطار اللي ممكن تقابلني
+
+63
+00:05:01,880 --> 00:05:06,000
+داخل المختبر؟ اللي هي خطر حدوث حرائق، خطر إيه يعني
+
+64
+00:05:06,000 --> 00:05:10,520
+كسر لمادة زجاجية، الأدوات الحادة، خطر انسكاب أي
+
+65
+00:05:10,520 --> 00:05:16,960
+مادة كيميائية، خطر الغاز و السوائل المضغوطة و خطر
+
+66
+00:05:16,960 --> 00:05:21,180
+الحرارة و البرودة، طالما أن الحرارة العالية و
+
+67
+00:05:21,180 --> 00:05:25,200
+البرودة العالية ممكن تسبب حروق للجسمخطر المواد
+
+68
+00:05:25,200 --> 00:05:28,080
+الكيميائية والمواد البيولوجية زي الفيروسات
+
+69
+00:05:28,080 --> 00:05:30,780
+والبكتيريا والفطريات والطفاليات اللي بتكون موجودة
+
+70
+00:05:30,780 --> 00:05:34,920
+داخل المختبر الإشعاع طيب و أي أنواع أخرى من
+
+71
+00:05:34,920 --> 00:05:38,920
+المخاطر لو كان في أنتي .. أنا كيف بدي أتجنب حدوث
+
+72
+00:05:38,920 --> 00:05:42,760
+حرائق أول حاجة المادة اللي بسميها flammable إيش
+
+73
+00:05:42,760 --> 00:05:47,600
+يعني flammable يعني مادة حارقة تمام أو مشتعلة طبعا
+
+74
+00:05:47,600 --> 00:05:50,800
+بدي أ .. لازم يكون في عندي منها في المختبر كميات
+
+75
+00:05:50,800 --> 00:05:55,620
+قليلة مافعش أخزنها بكميات كتير كبيرةتمام store in
+
+76
+00:05:55,620 --> 00:05:59,300
+special storage cabinet طبعا بحطها في كابينات خاصة
+
+77
+00:05:59,300 --> 00:06:04,220
+بيبقى محافظة على برودتها انها يعني موفرالي درجة
+
+78
+00:06:04,220 --> 00:06:08,980
+حرارة .. حرارة باردة عساس ان هاي المادة ما تشتعل و
+
+79
+00:06:08,980 --> 00:06:11,820
+طبعا لازم استخدم temperature controlled heating
+
+80
+00:06:11,820 --> 00:06:16,260
+source يعني .. يعني يكون في عندي قياس مستمر ل ..
+
+81
+00:06:16,290 --> 00:06:21,790
+الدرجة الحرارة داخل هاي ال cabinet ع أساس انه ما
+
+82
+00:06:21,790 --> 00:06:26,650
+يكون في عندى ارتفاع لدرجات الحرارة ممكن يسبب
+
+83
+00:06:26,650 --> 00:06:30,830
+اشتعالها حتى لما بدي اتعامل مع هاي المادة اللى
+
+84
+00:06:30,830 --> 00:06:34,750
+بنسميها فيلم بالمشتعلة زى الكحول مثلالازم بدي
+
+85
+00:06:34,750 --> 00:06:38,150
+أسخنها بستخ .. بسخنها باستخدام ال water bath بدل
+
+86
+00:06:38,150 --> 00:06:41,810
+ما أحطها مباشرة على النار وتشتعل لأ بستخدمها ..
+
+87
+00:06:41,810 --> 00:06:44,430
+بسخنها باستخدام water bath في كتير من المواد
+
+88
+00:06:44,430 --> 00:06:49,870
+بنسميها flammable مش بس كحول أحيانا ال ether ال ..
+
+89
+00:06:49,870 --> 00:06:55,950
+يعني ..ال Acetone كتير مواد هنتعامل معها بعض ال
+
+90
+00:06:55,950 --> 00:07:00,510
+acids هنتعامل .. نتعامل معهم بيعطينا تحدير أنه هذه
+
+91
+00:07:00,510 --> 00:07:03,230
+المادة flammable لما بدي سخنها بسخنها باستخدام
+
+92
+00:07:03,230 --> 00:07:06,670
+water bath يعني حمام مائي أفضل من ما أعرضها لدرجة
+
+93
+00:07:06,670 --> 00:07:10,810
+الحرارة مباشرةو بتتخزن ب cabinet و بنستخدم منها
+
+94
+00:07:10,810 --> 00:07:14,350
+كميات قليلة تمام؟ بتكون عندي بدل ما أنا أستخدم
+
+95
+00:07:14,350 --> 00:07:17,970
+tank داخل المختبر من الكحول مثلا لأ أنا ب .. ب ..
+
+96
+00:07:17,970 --> 00:07:21,950
+بخليها في المخزن و بجيب على المختبر bottles على جد
+
+97
+00:07:21,950 --> 00:07:26,490
+استخدامي و حاجتي make sure that you know what to
+
+98
+00:07:26,490 --> 00:07:30,510
+do طبعا إذا حدث حرائق لازم تكون تعرفي تعملي القاتل
+
+99
+00:07:30,510 --> 00:07:34,740
+if you have a fireif you hear a fire alarm إذا انت
+
+100
+00:07:34,740 --> 00:07:39,940
+كان في عندك حريق أو كان في عندك انذار لحدوث حريق
+
+101
+00:07:39,940 --> 00:07:43,620
+لازم تكوني بتعرفي إيش بدك تعملي، كيف بدك تتصرفي،
+
+102
+00:07:43,620 --> 00:07:48,160
+كيف يعني؟ يعني مثلا بتعرفي مكان ال fire .. ال fire
+
+103
+00:07:48,160 --> 00:07:51,580
+fighting equipmentتعرف في مكان أدوات اللي هي
+
+104
+00:07:51,580 --> 00:07:55,860
+محاربة الحدوث حرائق زي ال blanket البطامية أعرف
+
+105
+00:07:55,860 --> 00:08:01,400
+وان موجودة داخل المختبر النبوب .. النبوبة لإطفاء
+
+106
+00:08:01,400 --> 00:08:06,220
+الحرائق أيضا كمان أعرف وان موجودة في عندي أدوات
+
+107
+00:08:06,220 --> 00:08:09,940
+برضه بستخدمها لإطفاء أنواع كتير من الحرائق زي
+
+108
+00:08:09,940 --> 00:08:15,080
+الرمل و ال .. و أنابيب مي و أشياء هذه ده كنت عارفة
+
+109
+00:08:15,080 --> 00:08:15,900
+وان موجودة
+
+110
+00:08:19,070 --> 00:08:23,590
+و أيضا غير أن أنا بدأ أكون عارفة وين موجودة، بدي
+
+111
+00:08:23,590 --> 00:08:27,270
+أكون عارفة مثلا مخرج التوارق من وين، كل مختبر
+
+112
+00:08:27,270 --> 00:08:31,630
+بيكون إيه له في الخارج مخرج .. مخرج توارق موجود
+
+113
+00:08:31,630 --> 00:08:37,890
+فيه، فبالتالي لازم نكون بنعرف كيف نستخدمه تمام؟
+
+114
+00:08:37,890 --> 00:08:42,490
+هذا بالنسبالي الجملة اللي معناها know the location
+
+115
+00:08:42,490 --> 00:08:47,950
+of firefighting equipmentطيب طبعا في عندي كل نوع
+
+116
+00:08:47,950 --> 00:08:52,070
+حرائق أنا بستخدملها مادة لإطفاءها يعني مثلا ال
+
+117
+00:08:52,070 --> 00:08:55,250
+bucket of water بيكون في عندي بكتات للماء مع ..
+
+118
+00:08:55,250 --> 00:08:58,970
+معبقة بالماء طبعا ها دي بتستخدم to extinguish
+
+119
+00:08:58,970 --> 00:09:03,870
+paper and wood fire ل .. لحتى أطفئ الحرائق اللي
+
+120
+00:09:03,870 --> 00:09:07,270
+ناتج من اشتعال ورق أو خشب لو بنش خشب .. طبعا ..
+
+121
+00:09:07,710 --> 00:09:10,930
+يعني شبكت فيه نار و هي بدأ .. بلش يحترق أو أوراق
+
+122
+00:09:10,930 --> 00:09:14,950
+على طول بروح أش بجيب ال buckets of water و بفتحها
+
+123
+00:09:14,950 --> 00:09:22,390
+و بكبها عليها تمام؟ فبالتالي ال .. يعني الميها هي
+
+124
+00:09:22,390 --> 00:09:27,270
+مستحيل أستخدمها لحتى أطف حرائق ناتج عن الكهرة بل
+
+125
+00:09:27,270 --> 00:09:30,170
+أنها هي هتسبب انفجار يعني أو هت .. هتعمل mass
+
+126
+00:09:30,170 --> 00:09:34,970
+كهربائي فبالتالي يعني كل مادة تستخدم في نوع من
+
+127
+00:09:34,970 --> 00:09:38,440
+أنواع الحرائقال bucket of sand بكتات بيكون فيها
+
+128
+00:09:38,440 --> 00:09:43,660
+رمل طبعا هذه الرمل أو حتى تربة جافة dry soil طبعا
+
+129
+00:09:43,660 --> 00:09:52,300
+تستخدم للحرائق اللي ناتجة من اش السوائل
+
+130
+00:09:52,300 --> 00:09:57,080
+المشتعلة يعني لو كحولطبعا سائل هذا الكحول اشتعل
+
+131
+00:09:57,080 --> 00:10:00,620
+على طول بدي اروح على .. يعني انكب على البنش و
+
+132
+00:10:00,620 --> 00:10:04,020
+اشتعل فبدي اروح على طول احط عليه bucket of sand
+
+133
+00:10:04,020 --> 00:10:07,540
+يعني افتح الرقبة كده تحت الرمل و اكبها عليه علشان
+
+134
+00:10:07,540 --> 00:10:14,400
+يطفي fire blanket طبعا اللي هي البطانية المصنعة من
+
+135
+00:10:14,400 --> 00:10:19,650
+ال heavy cotton طبعاهي عبارة عن حاجة .. هي يعني
+
+136
+00:10:19,650 --> 00:10:22,610
+المادة اللي .. اللي مصنوعة منها هاي ال blankets
+
+137
+00:10:22,610 --> 00:10:26,910
+البطانيات fire retardant chemicals يعني من مواد
+
+138
+00:10:26,910 --> 00:10:34,370
+كيميائية يعني معيقة لنشوب الحرائق أو يعني إنها
+
+139
+00:10:34,370 --> 00:10:40,810
+طاردة للحرائق أو للنار فبالتالي بنستخدمها لما ..
+
+140
+00:10:43,410 --> 00:10:48,170
+بنطفي الحرائق سواء كانت يعني اشتعلت بالجسم أو لأ،
+
+141
+00:10:48,170 --> 00:10:54,650
+تمام؟ وش اسمه؟ ال dry powder chemical إذا طبعا في
+
+142
+00:10:54,650 --> 00:10:58,950
+بكيتات بيكون فيها dry powder، مواد powder كيميائية
+
+143
+00:10:58,950 --> 00:11:02,570
+بتستخدم علشان أطف فيها الحرائق اللي ناتجة من
+
+144
+00:11:02,570 --> 00:11:07,330
+اشتعال جهاز كهربائي أو حريق ناتج عن كهربا، تمام؟
+
+145
+00:11:07,330 --> 00:11:10,990
+إذا انتي عندى ال .. ال bucket of water علشان أطفي
+
+146
+00:11:11,130 --> 00:11:15,230
+ال .. الحرائق المشتعلة في ال paper و ال .. و ال
+
+147
+00:11:15,230 --> 00:11:21,170
+wood الخشب و ال sand للحرائق اللي مشتعلة في سوائل
+
+148
+00:11:21,170 --> 00:11:25,090
+و ال fire blanket لو كانت الحرائق لمثل جسمي أو
+
+149
+00:11:25,090 --> 00:11:31,590
+ملابسي و ال dry powder للحرائق الناتجة من التيار
+
+150
+00:11:31,590 --> 00:11:37,400
+الكهربائي، تمام؟طبعا عشان أنا أحمي نفسي من .. و
+
+151
+00:11:37,400 --> 00:11:42,480
+أنا بشتغل مع ال .. ال .. لها بنزن أو مع أي جهاز
+
+152
+00:11:42,480 --> 00:11:47,320
+بيعطيني حرارة أو بيعطي fire فبالتالي لازم أنا أربط
+
+153
+00:11:47,320 --> 00:11:51,320
+شعري للأعلى سواء كان للناس اللي مش محجبين يعني انا
+
+154
+00:11:51,320 --> 00:11:54,780
+حاكينها ان احنا محجبين و ماعنا هاي المشكلة طبعا و
+
+155
+00:11:54,780 --> 00:11:59,000
+انا بسخن في المحلول اذا كان .. لجأت لتسخين محلول
+
+156
+00:11:59,330 --> 00:12:03,070
+never look into container بطلعش في المحلول نفسه و
+
+157
+00:12:03,070 --> 00:12:06,290
+هو بيصخل لإن هو ممكن على طول يفور و ي .. و يلامس
+
+158
+00:12:06,290 --> 00:12:10,530
+وجهه never point the end of test tube being heated
+
+159
+00:12:10,530 --> 00:12:15,530
+to yourself or other يعني مثلا أنا بسخن في تيوب
+
+160
+00:12:15,530 --> 00:12:20,850
+على ال .. اللهب مش لازم فتحة التيوب تكون موجهة في
+
+161
+00:12:20,850 --> 00:12:24,910
+اتجاهي او باتجاه زميلتي لأ باتجاه الحائط لازم تكون
+
+162
+00:12:24,910 --> 00:12:29,470
+موجودة تمام؟never heat in a closed container
+
+163
+00:12:29,470 --> 00:12:32,630
+مابسخنش اللي أنا بيبي و هي مغلقة لإنها ممكن تنفجر
+
+164
+00:12:32,630 --> 00:12:33,250
+و تنكسر
+
+165
+00:12:37,010 --> 00:12:41,290
+طيب never leave a heat source unattended يعني
+
+166
+00:12:41,290 --> 00:12:45,330
+ماتسيبيش مصدر الحرارة أو مصدر اللهب متروك بدون
+
+167
+00:12:45,330 --> 00:12:48,290
+انتباه يعني احنا حكينا المحاضرة الماضية ممنوع اترك
+
+168
+00:12:48,290 --> 00:12:52,950
+لهب بنزين بدون يضل شغال و اروح اشوف شغل تاني او
+
+169
+00:12:52,950 --> 00:12:56,090
+اشتغل في مكان تاني لأ بخلص بسكر لهب بنزين و بأمن
+
+170
+00:12:56,090 --> 00:13:00,150
+الغاز بعدين بنتقل الى مكان اخرheated metal and
+
+171
+00:13:00,150 --> 00:13:05,730
+glass looks cool use forceps or gloves before
+
+172
+00:13:05,730 --> 00:13:11,070
+handling اذا انا بدى استخدم ال heat glass او metal
+
+173
+00:13:11,070 --> 00:13:14,230
+يعني بستخدم مادة معدنية او زجاجية ساخنة اما
+
+174
+00:13:14,230 --> 00:13:17,310
+باستخدام ال gloves او باستخدام ال forceps و هذا
+
+175
+00:13:17,310 --> 00:13:20,370
+كلام احنا حاكيناه don't place hot glassware
+
+176
+00:13:20,370 --> 00:13:23,670
+directly into the lab desk or in cold water
+
+177
+00:13:23,670 --> 00:13:28,960
+الزجاجيات الساخنة هذهمابحطهاش مباشرة على ال bench
+
+178
+00:13:28,960 --> 00:13:32,640
+علشان ما تحرق ال bench و بنفس الوقت ما بحطها على
+
+179
+00:13:32,640 --> 00:13:37,720
+ماء وط .. على ماء بارد يعني او ما بخلي الماء
+
+180
+00:13:37,720 --> 00:13:40,920
+البارد يلامسها لإنها ممكن تنكسر يعني نفس فكرة ال
+
+181
+00:13:40,920 --> 00:13:45,060
+birex إذا كان ساخن و لمس ماء باردnever heat the
+
+182
+00:13:45,060 --> 00:13:48,480
+bottom of the test tube طبعا انا مابركزش النار و
+
+183
+00:13:48,480 --> 00:13:52,360
+انا ب .. و انا حاطة التيوب على اللهب مابركزش النار
+
+184
+00:13:52,360 --> 00:13:55,960
+تكون فقط موجودة على قاع التيوب لأ بالعكس انا بمرر
+
+185
+00:13:55,960 --> 00:13:59,960
+التيوب على اللهب على أساس انه يكون في تسخين منتضم
+
+186
+00:13:59,960 --> 00:14:03,680
+لكل أجزاء التيوب مع بعضها البعضلأنه ممكن يسيطن
+
+187
+00:14:03,680 --> 00:14:09,380
+اتفور المادة او تنفجر التيوب او تنكسر فهذا خطأ
+
+188
+00:14:09,380 --> 00:14:12,980
+يعني خطأ لازم اوزع الحرارة يعني انا و انا ماسكة
+
+189
+00:14:12,980 --> 00:14:17,980
+التيوب زي هيك امررها على اللهب حتى تتوزع درجة
+
+190
+00:14:17,980 --> 00:14:23,360
+الحرارة بامتزام على مكونات التيوب طبعا ال sharp
+
+191
+00:14:23,360 --> 00:14:29,190
+objectsطبعا احنا هنستخدم شفرات هنستخدم needles ابر
+
+192
+00:14:29,190 --> 00:14:34,730
+مقصات في المختبر فبالتالي اول حاجة اذا انا بدي اقص
+
+193
+00:14:34,730 --> 00:14:38,710
+اي شي مش لازم انه يعني لازم يكون بعيد عن قصابي و
+
+194
+00:14:38,710 --> 00:14:43,370
+بعيد عن جسمي بحيث انه انا ما اجرح حالي دايما
+
+195
+00:14:43,370 --> 00:14:47,950
+الأدوات الحاد ده بنحملها carry sharp object with
+
+196
+00:14:47,950 --> 00:14:52,520
+point and tip facing down and awayنايما أنا بمسك
+
+197
+00:14:52,520 --> 00:14:55,740
+المقص بحيث أنه الجزء الحاد تبعه أو الشفرة أو
+
+198
+00:14:55,740 --> 00:14:59,620
+السكينة .. الجزء الحاد تبعه مشار إلى الأسفل أو
+
+199
+00:14:59,620 --> 00:15:03,500
+بعيد يعني مابروحش مثلا زميلتي اتناولني تحكيلي
+
+200
+00:15:03,500 --> 00:15:07,380
+هاتيلي المقص أروح الجزء الحاد أوجهه باتجاهها في
+
+201
+00:15:07,380 --> 00:15:10,620
+وجهها لإنها ممكن تكون هي مش من تبها و يأذيها
+
+202
+00:15:11,060 --> 00:15:16,040
+فبالتالي لازم يكون مشار إلى الأسفل. Never try to
+
+203
+00:15:16,040 --> 00:15:20,040
+touch falling sharp instrument. إذا انكسر أدوات
+
+204
+00:15:20,040 --> 00:15:28,700
+حادة زجاجية مثلا وجعة شفرة أو أدوات حادة، وجع بيكر
+
+205
+00:15:28,700 --> 00:15:33,660
+وانكسر، إشي زجاجي انكسر، مش لازم أنا أروح أنمسه
+
+206
+00:15:33,660 --> 00:15:38,580
+بإيدي نهائيا علشان ما أجرح نفسي.hold sharp
+
+207
+00:15:38,580 --> 00:15:42,260
+instrument only on the holder طبعا دايما الأدوات
+
+208
+00:15:42,260 --> 00:15:47,540
+الحادة هاي بيكون إلها يعني container خاص فيها و
+
+209
+00:15:47,540 --> 00:15:51,480
+باتخلص فيها بال container الخاص فيها و طبعا إذا
+
+210
+00:15:51,480 --> 00:15:57,340
+انت حصل عن الإلك جرح أو اتعرضتي لنخزة من قبرة أو
+
+211
+00:15:57,340 --> 00:16:02,400
+من شفرةلازم تحكي لل .. لل teacher يعني للمدرس و
+
+212
+00:16:02,400 --> 00:16:06,240
+بالتالي ت .. هو بي .. بيقوم معاكي بإجراءات
+
+213
+00:16:06,240 --> 00:16:10,360
+الإسعافات الأولية اللي بتتضمن أول حاجة انعصر الجرح
+
+214
+00:16:10,360 --> 00:16:15,360
+منيح عشان يطلع شوية دم و بعد هيك أغسل المكان
+
+215
+00:16:15,360 --> 00:16:19,380
+بالماء و الصابون و أعقم بالكحول و أحط bandage أو
+
+216
+00:16:19,380 --> 00:16:25,950
+لفاف أو plaster إذا لزم الأمرطبعا إحنا حكينا إنه
+
+217
+00:16:25,950 --> 00:16:30,770
+الأدوات الحادة ما بتلفها في سلة المهملات إنما إلها
+
+218
+00:16:30,770 --> 00:16:35,310
+box خاص فيها اسمه sharp box صندوق الأدوات الحادة
+
+219
+00:16:35,310 --> 00:16:41,110
+طبعا إذا كان تم انسكاب أي مادة كيميائية أول حاجة
+
+220
+00:16:41,110 --> 00:16:43,730
+بديش .. ما .. مابنفع أنا أتركها أروح أكمل شغل و
+
+221
+00:16:43,730 --> 00:16:47,070
+بعدين أرجعلها لإن ممكن يكون كتير في مشاكلعلى طول
+
+222
+00:16:47,070 --> 00:16:49,990
+بدي أعملها clear up on time يعني بدي أنظفها في
+
+223
+00:16:49,990 --> 00:16:54,630
+وقتها dispose any hazardous material and as toxic
+
+224
+00:16:54,630 --> 00:16:58,870
+waste و المادة اللي .. اللي انكبت أو انكسرت بتخلص
+
+225
+00:16:58,870 --> 00:17:02,690
+منها كأنها على أساس أنها هي toxic طبعا ال messy
+
+226
+00:17:02,690 --> 00:17:05,840
+workers اللي .. اللي ..الناس اللي بتشتغل بفوضى
+
+227
+00:17:05,840 --> 00:17:11,100
+دايما هم عبارة عن poor worker يعني شغلهم ما ..
+
+228
+00:17:11,100 --> 00:17:15,060
+مافيش إنتاجية، مافيش نتائج دقيقة، مافيش إشي صحيح
+
+229
+00:17:15,060 --> 00:17:17,900
+فبالتالي هذه مشكلتهم
+
+230
+00:17:20,330 --> 00:17:25,770
+طبعا خطر الوجود استوانات الغاز أو السوائل المضغوطة
+
+231
+00:17:25,770 --> 00:17:29,310
+داخل .. أو الغازات المضغوطة داخل المختبر أول حاجة
+
+232
+00:17:29,310 --> 00:17:32,510
+طبعا ممنوع أنا أستخدم هذه الاستوانات بدون ما أكون
+
+233
+00:17:32,510 --> 00:17:36,470
+متدرب عليها و لازم أقلل عددها minimize the number
+
+234
+00:17:36,470 --> 00:17:40,350
+in the laboratory أقلل عددها داخل المختبر ال
+
+235
+00:17:40,350 --> 00:17:45,990
+cylinder طبعا هي عبارة عن شئ ثقيل فبالتالي ما بنفع
+
+236
+00:17:45,990 --> 00:17:50,080
+أناإذا سقطت ممكن يعني على رجلي أو إشي ممكن تعمل
+
+237
+00:17:50,080 --> 00:17:53,960
+أداء كتير كبير فبالتالي لازم أنا لما أجي أحركها من
+
+238
+00:17:53,960 --> 00:17:57,380
+مكان إلى مكان ما أستخدم العضلات إنما أستخدم
+
+239
+00:17:57,380 --> 00:18:04,520
+literally يعني أروح أجيب ال .. يعني أداء ب ..
+
+240
+00:18:04,520 --> 00:18:10,200
+بيلها عجلات بتتحرك و ب .. بحط ال cylinder عليها و
+
+241
+00:18:10,200 --> 00:18:13,540
+بنقلها من مكان لآخر تبع .. باستخدام literally
+
+242
+00:18:13,540 --> 00:18:18,700
+تمام؟ensure that they are connected when in use
+
+243
+00:18:18,700 --> 00:18:22,100
+طبعا لازم انا اتأكد انها موصلة منيح اثناء
+
+244
+00:18:22,100 --> 00:18:25,740
+استخدامها ما .. يعني مايكونش في اشياء فالتة او
+
+245
+00:18:25,740 --> 00:18:31,960
+اشياء رخوة لأ لازم تكون محكومة الإغلاق و هي محكومة
+
+246
+00:18:31,960 --> 00:18:38,160
+الاتصال بالكوابل تبعتها او اللي هي بالانابيب
+
+247
+00:18:38,160 --> 00:18:42,640
+تبعتها use regulator طبعا لازم يكون في عندى منظم
+
+248
+00:18:42,640 --> 00:18:46,960
+ليلها عشان يتحكملي بكمية الغاز اللي بتطلعليو أيضا
+
+249
+00:18:46,960 --> 00:18:51,140
+إذا كان في عندي أي مشكلة أو أي تسريب لازم أنا
+
+250
+00:18:51,140 --> 00:18:55,120
+أعالج المشكلة ب .. ب .. باستمرار لإن حكينا تسريب
+
+251
+00:18:55,120 --> 00:18:56,600
+الغاز ممكن يعمل حرائق.
+
+252
+00:18:59,800 --> 00:19:04,860
+Cryogenics في عندي غازات طبعا هي liquid أنا بضغطها
+
+253
+00:19:04,860 --> 00:19:10,840
+لحتى أحافظ لحتى أخزن مثلا أشياء على درجات حرارة
+
+254
+00:19:10,840 --> 00:19:16,880
+كتير كتير قليلة يعني مثلا الخلايا جسم الإنسان لما
+
+255
+00:19:16,880 --> 00:19:20,120
+أنا بدي أخزنها بخزنها على سالب سبعين و سالب تمانين
+
+256
+00:19:20,120 --> 00:19:25,950
+طبعا ما بصلش لدرجات الحرارة هذه إلا باستخدامغازات
+
+257
+00:19:25,950 --> 00:19:30,310
+طبعا ال liquid gases طبعا liquid gases اللي هي
+
+258
+00:19:30,310 --> 00:19:35,130
+عبارة عن هي extremely cold بتكون باردة كتير و ممكن
+
+259
+00:19:35,130 --> 00:19:38,610
+تسبب حرائق في حرائق بسبب البرودة زي ما في حرائق
+
+260
+00:19:38,610 --> 00:19:41,390
+بسبب ارتفاع درجات الحرارة او اللي هي عند ال heat
+
+261
+00:19:41,390 --> 00:19:46,050
+السخونة liquid gas evaporate ممكن انها هي هذه ال
+
+262
+00:19:46,050 --> 00:19:49,810
+liquid gases تتبخر و تعمللي acidification اختناق
+
+263
+00:19:49,810 --> 00:19:53,010
+فبالتالي لازم انا احافظ عليها ان هي ما تتبخر و ما
+
+264
+00:19:53,010 --> 00:19:58,070
+تتسربو تكون محكمة الإغلاق داخل الأنابيب تبعتها و
+
+265
+00:19:58,070 --> 00:20:01,630
+طبعا ما بستخدمها إلا ب training خاص فيها زي ال
+
+266
+00:20:01,630 --> 00:20:07,230
+liquid nitrogen أكيد سمعتوا عنهو بعدين ممكن انها
+
+267
+00:20:07,230 --> 00:20:11,130
+تكون قابلة للانفجار او الاحتراق ال electrical
+
+268
+00:20:11,130 --> 00:20:16,510
+equipment طبعا كيف انا اتعامل الحد من خطر الدرجات
+
+269
+00:20:16,510 --> 00:20:22,950
+او خطر الكهرباء اول حاجة بدي اعمل visual check على
+
+270
+00:20:22,950 --> 00:20:25,790
+ال electrical equipment حكينا المحاضرة الماضية انه
+
+271
+00:20:25,790 --> 00:20:28,570
+الأدوات الكهربائية لازم باستمرار انا اشيك عليها
+
+272
+00:20:28,820 --> 00:20:33,800
+أفقي نظرة عليها، أشوف درجة حرارتها، أشوف هل هي
+
+273
+00:20:33,800 --> 00:20:37,160
+مشبوك، هل هي بتشتغل بانتظام أو لأ، فيها خلال ما أو
+
+274
+00:20:37,160 --> 00:20:41,120
+فيها عطل، بتعطيني alarm يعني انذار انه والله انه
+
+275
+00:20:41,120 --> 00:20:44,740
+هي .. هو هذا الجهاز خربان، فهذا كله لازم أكون
+
+276
+00:20:44,740 --> 00:20:49,600
+متعاملة معاه، بعدين كمان all portable electrical
+
+277
+00:20:49,600 --> 00:20:53,340
+equipment must have a current path test يعني لازم
+
+278
+00:20:53,340 --> 00:20:58,930
+يكون فيه على كل ال .. الأجهزة الكهربائيةعبارة عن
+
+279
+00:20:58,930 --> 00:21:02,410
+sticker بسميه portable appliance testing يعني هذا
+
+280
+00:21:02,410 --> 00:21:05,730
+ال sticker بيحكيلي أن هو شغال ولا مش شغال، إذا كان
+
+281
+00:21:05,730 --> 00:21:11,370
+فيه مشكلة أو لأ، إمتى تم تشغيله، طبعا إيش محتاج
+
+282
+00:21:11,370 --> 00:21:14,130
+ملاحظات أثناء تشغيله، هذه كلها بتكون موجودة على ال
+
+283
+00:21:14,130 --> 00:21:17,830
+portable appliance testing sticker طبعا never use
+
+284
+00:21:17,830 --> 00:21:21,110
+defective equipment إذا الجهاز مكتوب لك عليه أن هو
+
+285
+00:21:21,110 --> 00:21:26,400
+هذا defective، فيه خللعطلان major بتستخدميه نهائيا
+
+286
+00:21:26,400 --> 00:21:31,560
+ما تحاولي فيه تمام و طبعا only electrical plugs
+
+287
+00:21:31,560 --> 00:21:34,840
+are to be placed into electrical outlet طبعا بس
+
+288
+00:21:34,840 --> 00:21:38,060
+الأداء الأسلاك الكهربائية هي اللي احنا الكبش هي
+
+289
+00:21:38,060 --> 00:21:44,570
+اللي بنحطها داخل ال ..يعني داخل الطيار الكهربائي
+
+290
+00:21:44,570 --> 00:21:51,290
+أما الأدوات الحادة لأ أقلام أشياء تانية لأ unplug
+
+291
+00:21:51,290 --> 00:21:54,570
+electrical equipment after use بعد ما أخلص استخدام
+
+292
+00:21:54,570 --> 00:21:59,450
+الجهاز لازم طبعا أرفع الأكوابل أفصلها ما أخليها
+
+293
+00:21:59,450 --> 00:22:03,110
+مشبوكة بالكهربا و طبعا كل ال electrical cord
+
+294
+00:22:03,110 --> 00:22:08,080
+الأحبال و الأسلاك الكهربائيةلازم تكون بعيدة عن
+
+295
+00:22:08,080 --> 00:22:12,260
+الماء و لازم طبعا الماء ألمسها إذا كانت هي مكشوفة
+
+296
+00:22:12,260 --> 00:22:20,560
+طيب ال chemical safety ال chemical safety أول حاجة
+
+297
+00:22:20,560 --> 00:22:23,240
+بحكيلك عن المواد الكيميائية طبعا هتكون عندنا
+
+298
+00:22:23,240 --> 00:22:28,500
+زجاجات كيميائية زي هذا الشكل بهك بهذا الشكل هك
+
+299
+00:22:30,830 --> 00:22:35,650
+طبعا زي ما أنتوا شايفين و هذه الزجاجات عليها
+
+300
+00:22:35,650 --> 00:22:40,430
+علامات طبعا العلامات هذه أو يعني ال label هذا
+
+301
+00:22:40,430 --> 00:22:46,350
+الأوراق الملثقة على الزجاجات
+
+302
+00:22:46,350 --> 00:22:50,810
+فيها معلومات بتخص كل .. كل إشي بيتعلق بالمادة
+
+303
+00:22:50,810 --> 00:22:54,770
+الكيميائية، استخدامها، درجة حرارة حفظها، تاريخ
+
+304
+00:22:54,770 --> 00:22:59,970
+فتحها، تاريخ انتهاءهاتركيبتها الكيميائية تعليمات
+
+305
+00:22:59,970 --> 00:23:06,230
+لا استخدامها تحذيرات استخدامها بشكل خطأكل إشي
+
+306
+00:23:06,230 --> 00:23:09,230
+بيكون موجودة على هذا ال label فبالتالي لازم انا
+
+307
+00:23:09,230 --> 00:23:12,650
+أقرأ ال label هذا twice بده أقرأه بدل المرة مرتين
+
+308
+00:23:12,650 --> 00:23:17,850
+علشان أتأكد أن أنا فاهمة كل إشي بيتعلق بهذه المادة
+
+309
+00:23:17,850 --> 00:23:21,790
+قبل استخدامها طبعا only use the type and amount of
+
+310
+00:23:21,790 --> 00:23:25,490
+chemical instructed طبعا أنا بستخدم نفس نوع المادة
+
+311
+00:23:25,490 --> 00:23:30,150
+و نفس الكمية في التفاعل الكيميائي مثلا حكالي والله
+
+312
+00:23:30,150 --> 00:23:33,950
+في التفاعل الكيميائي ضيفي 2 ml of etherدور
+
+313
+00:23:33,950 --> 00:23:38,650
+بالخزانة فتش فتش فتش مافيش ether لاجت acetone يلا
+
+314
+00:23:38,650 --> 00:23:41,610
+ماهو ether ولا acetone إخوان بعض خليني أضيف
+
+315
+00:23:41,610 --> 00:23:46,390
+acetone اتنين ML acetone والله ما أنا مش عاجبنك
+
+316
+00:23:46,390 --> 00:23:49,810
+أنه أحط اتنين ML مش هيك مش حاسة أنه النتيجة هتطلع
+
+317
+00:23:49,810 --> 00:23:54,080
+مظبوطةخليني أحط تلاتة أمال، هذا خطأ، لازم أستخدم
+
+318
+00:23:54,080 --> 00:23:57,340
+نفس المادة الكيميائية المشارقليها بالتجربة و نفس
+
+319
+00:23:57,340 --> 00:24:00,980
+الكمية، مابنفعش انا هيك يعني يكون في عندي حس الطبخ
+
+320
+00:24:00,980 --> 00:24:04,820
+و الحس الطباخ و أحس أنه انا اتنين أمال، لأ لأ مش
+
+321
+00:24:04,820 --> 00:24:11,510
+كافين، زودوا، لأ بنفعشتمام؟ ف .. و .. و كمان هذه
+
+322
+00:24:11,510 --> 00:24:14,710
+المعلومة مرت معانا never touch ممنوع ألمس ممنوع
+
+323
+00:24:14,710 --> 00:24:19,070
+أتضوخ ممنوع أشتم أي مادة كيميائية ممنوع أخلطهم مع
+
+324
+00:24:19,070 --> 00:24:22,410
+بعض المواد الكيميائية بدون ما ال .. ال mess تحكيلي
+
+325
+00:24:22,410 --> 00:24:26,590
+انا حكيتلك اعملي .. ضيفي ال .. المادة A على المادة
+
+326
+00:24:26,590 --> 00:24:30,990
+B و .. و .. و .. و اعمليلهم mix كويس بتضيفيهمبدون
+
+327
+00:24:30,990 --> 00:24:35,830
+ما انا احكي رجاء الشخص الكيميائي او ال .. ال .. ال
+
+328
+00:24:35,830 --> 00:24:39,090
+.. الانسان الكيميائي اللي جواتك اقتليه قبل لاتفوت
+
+329
+00:24:39,090 --> 00:24:44,500
+المختبر لإنه هيودينا في دهيا.transfer chemical
+
+330
+00:24:44,500 --> 00:24:47,920
+carefully طبعا بدي أنقل المواد الكيميائية من مكان
+
+331
+00:24:47,920 --> 00:24:51,540
+إلى أخر بحذر بحيث انه انا يعني ما أصطدم مثلا
+
+332
+00:24:51,540 --> 00:24:55,220
+بزميلتي و انا ماشية اكب عليها او ينكب عليا keep
+
+333
+00:24:55,220 --> 00:24:58,060
+lid on chemical container لازم تكون المواد
+
+334
+00:24:58,060 --> 00:25:02,500
+الكيميائية مغطاة تمام handal acid and alkalis
+
+335
+00:25:02,500 --> 00:25:07,480
+firmly upright and dry hand لازم انا بستخدم المواد
+
+336
+00:25:07,480 --> 00:25:12,700
+الحمضية و القاعدية بأيدي جافةو بأيدي لابسة gloves
+
+337
+00:25:12,700 --> 00:25:18,620
+و باستخدام التعليمات اللي حتى أحافظ على نفسي never
+
+338
+00:25:18,620 --> 00:25:22,300
+pour water into strong acid because the danger of
+
+339
+00:25:22,300 --> 00:25:25,320
+reaction and splashing يعني مابعرفش عندي strong
+
+340
+00:25:25,320 --> 00:25:29,940
+acid HCl ولا H2SO4 و أنا بدي أخففه و أضيف عليه
+
+341
+00:25:29,940 --> 00:25:35,240
+water مباشرة لأ مابنفعشيعني لإنه هذا ممكن يقدي إلى
+
+342
+00:25:35,240 --> 00:25:39,100
+إيش انه يصير splashing تطير لأ ال acid و يبتدي
+
+343
+00:25:39,100 --> 00:25:42,280
+يصير حارق طبعا انا كده طبعا إيش بدي أعمل لما بدي
+
+344
+00:25:42,280 --> 00:25:46,480
+أخفف ال acid بروح بضيف ال acid للماء و ليس الماء
+
+345
+00:25:46,480 --> 00:25:50,530
+لل acid تتذكر المحاضرة الماضية إيش حكيتلكم؟حكيتلكم
+
+346
+00:25:50,530 --> 00:25:54,010
+حتى ترتيب الخطوات كويس عشان هيك لما .. لما هنشتغل
+
+347
+00:25:54,010 --> 00:25:57,630
+مع بعض بالمختبر هنكون ماشيين خطوة خطوة كلكوا يلا
+
+348
+00:25:57,630 --> 00:26:01,010
+اضيفوا المادة الكولانية الكل ده يلا ننتقل للخطوات
+
+349
+00:26:01,010 --> 00:26:05,630
+التانية وها كذا لإنه فعليا انا لما اجي احكي للطالب
+
+350
+00:26:05,630 --> 00:26:10,510
+اضيفي اخفف ال acid ب water فعليا ان انت تضيفي ال
+
+351
+00:26:10,510 --> 00:26:14,670
+water لل acid اشي مصيبةلكن انك انت مثلا ضيف ال
+
+352
+00:26:14,670 --> 00:26:19,110
+acid لالماء بالتدريج و ع الجدار و شوي شوي هيك
+
+353
+00:26:19,110 --> 00:26:23,310
+بيكون بطريقة صحيحة احنا بنخفف ال acid و ما .. بنسي
+
+354
+00:26:23,310 --> 00:26:27,570
+.. بنعرض نفسنا لخطر انه يتطير المادة الحامضية قوية
+
+355
+00:26:27,570 --> 00:26:31,350
+هاي الحارقة و انه نؤذي نفسنا او نؤذي زملائنا او
+
+356
+00:26:31,350 --> 00:26:32,430
+المكان المحيط فينا
+
+357
+00:26:35,310 --> 00:26:38,770
+طيب don't keep acid and alkalis in bottle with the
+
+358
+00:26:38,770 --> 00:26:42,990
+ground glasses to bare طيب ال .. ال .. مواقع
+
+359
+00:26:42,990 --> 00:26:47,230
+الأحماض و القواعد هذه بنحطها احنا في أنابيب زجاجية
+
+360
+00:26:47,230 --> 00:26:49,990
+بس لازم الأنابيب الزجاجية تكون غطيتها plastic
+
+361
+00:26:49,990 --> 00:26:54,430
+ماتكونش غطيتها glass لإن ممكن ال glass هذه تصير ..
+
+362
+00:26:54,430 --> 00:26:59,050
+يعني تصير ملتصقة و صعب تفتح they .. they may get
+
+363
+00:26:59,050 --> 00:27:03,590
+stuck ممكن تلتصق يعني تصير عالقة مش راضية تفتح
+
+364
+00:27:03,590 --> 00:27:07,500
+النبوبةconsider all chemical dangerous طبعا انت
+
+365
+00:27:07,500 --> 00:27:10,060
+بديك تتعاملي مع كل المواد الكيميائية انها خاطرة
+
+366
+00:27:10,060 --> 00:27:14,340
+علشان تكون بتحمي نفسك كلها تتعاملي معاها بحضر و
+
+367
+00:27:14,340 --> 00:27:18,380
+طبعا بنستخدم ال by bit that are plugged with non
+
+368
+00:27:18,380 --> 00:27:22,260
+absorbent cotton wool with rubbertube attachment
+
+369
+00:27:22,260 --> 00:27:26,260
+بنستخدم الأنواع مخصصة من ال by بتعشان ننقل الحجوم
+
+370
+00:27:26,260 --> 00:27:30,260
+فيها يعني ال acid و ال base هناخدها بالمختبر و
+
+371
+00:27:30,260 --> 00:27:33,460
+أيضا every container of chemical is labeled with
+
+372
+00:27:33,460 --> 00:27:36,820
+by manufacturer حكينا إنه كل مادة كيميائية
+
+373
+00:27:36,820 --> 00:27:40,600
+الهوايقة تبعها بيكون عليه label جاي من الشركة
+
+374
+00:27:40,600 --> 00:27:45,160
+المصنعة كل إشي محطوط عليه مادة الكيميائية اسمها
+
+375
+00:27:45,160 --> 00:27:51,010
+name of chemical nameاسم و عنوان و ال emergency
+
+376
+00:27:51,010 --> 00:27:55,210
+phone للمادة المصنعة .. للشركة المصنعة يعني ال ..
+
+377
+00:27:55,210 --> 00:27:58,790
+ال .. الاسم و العنوان و التليفون، طوارئ تبع الشركة
+
+378
+00:27:58,790 --> 00:28:04,290
+المصنعة في حال لو حدث مشكلةphysical and health
+
+379
+00:28:04,290 --> 00:28:08,090
+hazard يعني هذه المادة ده ايش خطرها عليكي ممكن
+
+380
+00:28:08,090 --> 00:28:11,730
+تكون موجودة بيعيقيكي تعليمات ان هي ممكن تكون حارقة
+
+381
+00:28:11,730 --> 00:28:14,970
+ممكن تكون بتسبب تلف للانتجار ممكن تكون سامة هذا
+
+382
+00:28:14,970 --> 00:28:17,130
+كله بيكون موجود على ال bottle من برة عشان هيكي
+
+383
+00:28:17,130 --> 00:28:19,850
+احنا لازم نقرأ هاي ال .. ال label اللي موجودة على
+
+384
+00:28:19,850 --> 00:28:22,900
+ال bottlefirst-hand instruction بيعطيكي تعليمات
+
+385
+00:28:22,900 --> 00:28:25,560
+ايضا اسعاف اول لو هاي المادة انسكبت عليكي ايش
+
+386
+00:28:25,560 --> 00:28:29,200
+تعملي او انت بالغلط استنشقتيها او بالغلط ايش لمست
+
+387
+00:28:29,200 --> 00:28:32,700
+ايداكي و ايضا proper handling and storage
+
+388
+00:28:32,700 --> 00:28:35,960
+instruction و كيف انت تتعاملي معاها تستخدميها و
+
+389
+00:28:35,960 --> 00:28:39,520
+كيف تخزنيها ايضا هذه التعليمات كلها بتكون موجودة
+
+390
+00:28:39,520 --> 00:28:40,680
+على ال label
+
+391
+00:28:44,270 --> 00:28:48,650
+physical safety طيب handle all equipment carefully
+
+392
+00:28:48,650 --> 00:28:53,110
+بدا اتعامل مع كل الأدوات عندي و الأجهزة بحذر و
+
+393
+00:28:53,110 --> 00:28:56,990
+طبعا الأسلاك اللي بتكون موجودة داخل المختبر انا مش
+
+394
+00:28:56,990 --> 00:29:01,240
+لازم انا امددها و تكون بتقطع طريقة عشانما اتعرقل
+
+395
+00:29:01,240 --> 00:29:04,980
+فيها و اجعل خصوصا و انا مسك ادوات زجاجية او محالي
+
+396
+00:29:04,980 --> 00:29:09,580
+بنجلهم من بنش ل بنش push all stools in and out the
+
+397
+00:29:09,580 --> 00:29:13,340
+way طبعا لازم تكون الكراسي مجرد ما انت تجومي عن
+
+398
+00:29:13,340 --> 00:29:17,890
+الكرسي اتفوتي في مكانه بحيث انه مايكنمرمى فى نص
+
+399
+00:29:17,890 --> 00:29:21,710
+اللى بتيجى بزميلتك تنخبط فيه او ماشية مش من تبهاله
+
+400
+00:29:21,710 --> 00:29:27,310
+انها يعني تسقط او يجع منها اشي او ينكسر اشي او
+
+401
+00:29:27,310 --> 00:29:32,330
+ينكب عليها اشي تمام؟ و ايضا لازم يكون المكان بشكل
+
+402
+00:29:32,330 --> 00:29:38,080
+عاماللي بنشتغل فيه tidy ال work place تبعي المكان
+
+403
+00:29:38,080 --> 00:29:42,920
+اللي عامل لازم يكون tidy نظيف لازم أتخلص من ال
+
+404
+00:29:42,920 --> 00:29:47,120
+waste أول بأول clear up waste و طبعا حكينا احنا أن
+
+405
+00:29:47,120 --> 00:29:50,380
+ال waste تبع الفضلات كل نوع أتخلص فيه في المكان
+
+406
+00:29:50,380 --> 00:29:56,400
+المناسب لإله و أيضا لإن ال tidy laboratory المختبر
+
+407
+00:29:56,400 --> 00:30:01,640
+النظيف avoid accidents بيمنعني من حدوث أو بيجنبني
+
+408
+00:30:01,640 --> 00:30:07,900
+من حدوثال .. الحوادث او المخاطر تمام ال laboratory
+
+409
+00:30:07,900 --> 00:30:11,680
+equipment never use any laboratory equipment
+
+410
+00:30:11,680 --> 00:30:16,240
+unless you are trained طبعا اي جهاز او اي ادا
+
+411
+00:30:16,240 --> 00:30:19,460
+ممنوعة تستخدميها الا لما تكوني اتدربتي عليها او
+
+412
+00:30:19,460 --> 00:30:23,300
+انا اعطيتك اشارة انك تستخدميها او انه علمتك
+
+413
+00:30:23,300 --> 00:30:29,580
+شريحتليك كيفية استخدامهاو بالتالي كمان لإنه ..
+
+414
+00:30:29,580 --> 00:30:32,720
+يعني ان انت تئدي نفسك او انك تخربي الجهاز او تخربي
+
+415
+00:30:32,720 --> 00:30:37,120
+ال .. الأداء او انك انت مثلا اي حد يطلق ضرر معين
+
+416
+00:30:37,120 --> 00:30:41,920
+نتيجة استخدامك الخاطئ لها، هاد طبعا تمانه او عراقه
+
+417
+00:30:41,920 --> 00:30:46,840
+وخيمة.ال hazard symbols ايش يعني ال hazard
+
+418
+00:30:46,840 --> 00:30:52,420
+symbols؟ هي عبارة عن symbols designed to warn
+
+419
+00:30:52,420 --> 00:30:57,540
+about hazardous material تمام؟ و location and or
+
+420
+00:30:57,540 --> 00:31:02,600
+objects يعني هي عبارة عنrecognizable symbols رموز
+
+421
+00:31:02,600 --> 00:31:08,920
+يمكن إدراكها ومصممة design to مصممة لتحذرني to
+
+422
+00:31:08,920 --> 00:31:12,760
+warn على إيش تحذرني على ال hazardous material
+
+423
+00:31:12,760 --> 00:31:19,520
+المواد الخطرة أماكنها و الاشياء اللي موجودة فيها و
+
+424
+00:31:19,520 --> 00:31:24,840
+أيضا لتحذرني انه على خطر ال electric current
+
+425
+00:31:24,840 --> 00:31:29,060
+التيار الكهربائي والسموم والإشعاعاتهذه كلها بتكون
+
+426
+00:31:29,060 --> 00:31:32,560
+موجودة طبعا في المختبر و حكينا أنها بتكون موجودة
+
+427
+00:31:32,560 --> 00:31:35,420
+على الحائط على ال cabinets على الأدوات الزجاجية
+
+428
+00:31:35,420 --> 00:31:41,040
+على المواد الكيميائية لحتى اتحذرني من خطر و شرحنا
+
+429
+00:31:41,040 --> 00:31:45,940
+هذه ال symbols أو هذه الرموز في المحاضرة الماضية
+
+430
+00:31:47,950 --> 00:31:54,770
+تمام ال first aid كيف هي عملية الإسعاف الأولى إذا
+
+431
+00:31:54,770 --> 00:32:00,450
+حدث hazard إذا حدث خطر معين داخل المختبر طبعا أول
+
+432
+00:32:00,450 --> 00:32:07,910
+حاجة أنه لازم أكون يعني بحكيلك أنه ماعرفت ماذا يجب
+
+433
+00:32:07,910 --> 00:32:13,090
+عليك أنت تتصرف يقيك العواقب تمام knowing what to
+
+434
+00:32:13,090 --> 00:32:17,210
+do immediately ماعرفت إيش لازم تعملي في الحالif an
+
+435
+00:32:17,210 --> 00:32:21,770
+accident occur إذا حدث حادث أو حصل حادث تمام can
+
+436
+00:32:21,770 --> 00:32:25,690
+help you بتساعدك to reduce suffering تقلل معاناتك
+
+437
+00:32:25,690 --> 00:32:28,090
+و تقلل من ال serious damage أو ال serious
+
+438
+00:32:28,090 --> 00:32:31,530
+accidents أو العواقب ال consequences اللي ممكن
+
+439
+00:32:31,530 --> 00:32:38,010
+تحدثلك من هذه ال accidents تمام فبالتالي ب .. في
+
+440
+00:32:38,010 --> 00:32:42,070
+بعض الحالات ال first aid الإسعاف الأولى can be
+
+441
+00:32:42,070 --> 00:32:46,850
+life saving يعني ممكن يع .. يعنيينقذ حياة الشخص زي
+
+442
+00:32:46,850 --> 00:32:50,210
+مثلا إن أنا أعمل control of bleeding شخص صار عنده
+
+443
+00:32:50,210 --> 00:32:55,710
+cut injury جرح نتيجة إخدامه أدى حد بشكل خطأ لو أنا
+
+444
+00:32:55,710 --> 00:32:59,410
+عملت control لل bleeding هذا معناه .. معناه انا ..
+
+445
+00:32:59,410 --> 00:33:04,770
+انا حافظت على حياة الشخص هذا تمام فبالتالي لازم ال
+
+446
+00:33:04,770 --> 00:33:10,490
+laboratory worker لازم يكون عندهمتعليمات حوالين
+
+447
+00:33:10,490 --> 00:33:14,270
+الإسعافات الأولية و أعتقد أنتوا عندكم مادة كاملة
+
+448
+00:33:14,270 --> 00:33:18,310
+عن الإسعافات الأولية بتعلمكم في إيش ممكن .. كيف
+
+449
+00:33:18,310 --> 00:33:23,570
+ممكن تتصرفوا في أكتر من موقف لحتى يكون في عندكم
+
+450
+00:33:23,570 --> 00:33:28,660
+خلفية how to first aidmyself and my colleagues
+
+451
+00:33:28,660 --> 00:33:35,640
+طبعا لازم نعرف احنا اش منا نستخدم اش منا نعمل او
+
+452
+00:33:35,640 --> 00:33:38,780
+اش منا نتصرف في ال emergency action في حالات
+
+453
+00:33:38,780 --> 00:33:43,300
+التوارق برضه كمان لو مثلا عندي مريض أغم عليه
+
+454
+00:33:43,300 --> 00:33:49,700
+collapse مريض اختنق مريض قدردت فعل عكسية اثناء
+
+455
+00:33:49,700 --> 00:33:54,340
+مثلا سحب عينة الدم بخاف هو عادي بتصيرفهذه كلها
+
+456
+00:33:54,340 --> 00:33:58,500
+برضه كمان لازم أكون بعرف أتصرف فيها المواقف هاي
+
+457
+00:33:58,500 --> 00:34:04,100
+طبعا لازم يكون في عندى adequately equipped first
+
+458
+00:34:04,100 --> 00:34:08,180
+aid box تمام لازم يكون عندى صندوق للإسعافات
+
+459
+00:34:08,180 --> 00:34:11,900
+الأولية adequately equipped adequate يعني كافي
+
+460
+00:34:11,900 --> 00:34:15,300
+equipped محوج يعني محوج بشكل كافي يعني موجود فيه
+
+461
+00:34:15,300 --> 00:34:18,830
+كل الأدواتبشكل كافي فيه ال plasters و ال .. و
+
+462
+00:34:18,830 --> 00:34:23,870
+الشاشة و الكحول و ال iodine و ال .. و فيه كل إشي
+
+463
+00:34:23,870 --> 00:34:29,330
+اللي بيتعلق بالإسعافات الأولية ماسكات بلاسترات كل
+
+464
+00:34:29,330 --> 00:34:34,610
+إشي تمام؟و لازم هذا ال box باستمرار باستمرار أنا
+
+465
+00:34:34,610 --> 00:34:39,810
+أعمله examine عشان أشيك عليه إيش نقص منه أ .. أ ..
+
+466
+00:34:39,810 --> 00:34:44,590
+أعبيه مرتان و أخليه جاهز و يخليه متوفر فيه كل
+
+467
+00:34:44,590 --> 00:34:48,810
+الأدوات اللي بتكون موجودة عندىطبعا لازم يكون هذا
+
+468
+00:34:48,810 --> 00:34:52,010
+ال box مصنوع من ال metal أو ال plastic علشان ما
+
+469
+00:34:52,010 --> 00:35:01,470
+يتكسر و برضه كمان عشان .. و لازم يكون نظيف و مغلق
+
+470
+00:35:01,470 --> 00:35:06,610
+و موجود في مكان الكل بيعرف يصله لحتى نسهل من عملية
+
+471
+00:35:06,720 --> 00:35:10,280
+ال first aiding اللي هي الإسعافات الأولية طبعا إيش
+
+472
+00:35:10,280 --> 00:35:15,480
+مكونات ال .. الإسعافات .. صندوق الإسعافات الأولية
+
+473
+00:35:15,480 --> 00:35:19,780
+هى بيكون لازم يكون فيه sterile dressing يعني شاش
+
+474
+00:35:19,780 --> 00:35:23,970
+معقم عشان نغطي الجروح to cover woundabsorbent
+
+475
+00:35:23,970 --> 00:35:27,750
+cotton .. cotton عشان يمتص السوائل أو المواد
+
+476
+00:35:27,750 --> 00:35:31,430
+المعاقمة أو أي إشي لازم يكون في عن .. عندي roll
+
+477
+00:35:31,430 --> 00:35:35,550
+bandage اللي هي اللزج اللي عامل زي رولات أو حتى
+
+478
+00:35:35,550 --> 00:35:38,890
+اللي ماخد شكل المتلتات زي اللي بنحط على العين أو
+
+479
+00:35:38,890 --> 00:35:43,310
+إشياسترايل ايد باد لازم برضه كمان قطن خاص بالعين
+
+480
+00:35:43,310 --> 00:35:51,670
+معقّم رول أدهيس الشاش لاصق او شريط لاصق سيزورز
+
+481
+00:35:51,670 --> 00:35:54,890
+مقصات لازم يكون موجود فيه Sodium Bicarbonate
+
+482
+00:35:54,890 --> 00:35:58,470
+Powder و Boric Acid Powder و 5% Acetic Acid و
+
+483
+00:35:58,470 --> 00:36:01,810
+Magnesium Hydroxide Suspension طبعا هذه الأربع
+
+484
+00:36:01,810 --> 00:36:05,590
+محليل او المواد الكيميائية راح نعرف استخدامها في
+
+485
+00:36:05,590 --> 00:36:06,830
+ال slides الجاية
+
+486
+00:36:09,620 --> 00:36:15,030
+تمامإيش بحدث لو صار في عندي cut جرح و bleeding إيش
+
+487
+00:36:15,030 --> 00:36:18,330
+بيصير؟ أول إشي بدي أغسل المكان بالماء و الصابون،
+
+488
+00:36:18,330 --> 00:36:21,670
+تمام؟ بعدين حكينا apply pressure with a piece of
+
+489
+00:36:21,670 --> 00:36:26,970
+cotton، بدي أسكر الجرح بقطعة قطن، تمام؟ أعقم
+
+490
+00:36:26,970 --> 00:36:31,910
+المكان و ألفه، أعقمه ب iodine و ألفه، تمام؟ cover
+
+491
+00:36:31,910 --> 00:36:36,070
+.. هي هنا disinfect، بعقم و cover، بلفه، تمام؟
+
+492
+00:36:36,070 --> 00:36:43,710
+بالشاشة إذا كان الجرحطبعا، يعني أنا انجرحت بمادة
+
+493
+00:36:43,710 --> 00:36:48,390
+ملوثة، إيش أعمل؟ أولا بدي أخسل إيدي بالماء
+
+494
+00:36:48,390 --> 00:36:52,090
+والصابون، بعدين بدي أعمل encouraging bleeding of
+
+495
+00:36:52,090 --> 00:36:55,450
+two minutes، يعني أنا بدي أعصر إيدي و أخليها تنزف
+
+496
+00:36:55,450 --> 00:36:59,770
+لمدة two minutes، طبعا، ليش؟ لإن الدم هذا اللي
+
+497
+00:36:59,770 --> 00:37:03,680
+بيطلع من الجروح بياخد ال contaminationالمواد
+
+498
+00:37:03,680 --> 00:37:07,540
+الملوثة اللي .. اللي دخلت جوا الجرح و بطلعها لبره،
+
+499
+00:37:07,540 --> 00:37:11,200
+زي كأني أنا قاعدة بعمل washing بال bleeding تبعي،
+
+500
+00:37:11,200 --> 00:37:16,600
+تمام؟ بخسل تاني بالماء و الصابون و برجع بطبق نفس
+
+501
+00:37:16,600 --> 00:37:20,440
+الأدوات و إذا كان الجارح عميق ممكن أنا أسأل .. او
+
+502
+00:37:20,440 --> 00:37:26,080
+يعني الدكتور يجي يعمل قطب غرز طبية او .. او ما
+
+503
+00:37:26,080 --> 00:37:26,500
+شابه
+
+504
+00:37:29,320 --> 00:37:35,180
+طبعا، لو كان في عندي حروق باستخدام الحرارة، لو حدث
+
+505
+00:37:35,180 --> 00:37:37,960
+حرق باستخدام الحرارة، أنا لمست اشي ساخن بدون ما
+
+506
+00:37:37,960 --> 00:37:42,040
+أنتبه، إيش بدي أعمل؟ على طول immerse the burnt
+
+507
+00:37:42,040 --> 00:37:46,180
+area، المكان اللي صار له احتراقعلى طول بغمروا تحت
+
+508
+00:37:46,180 --> 00:37:52,980
+الماء البارد و طبعا or apply soaked bed with cold
+
+509
+00:37:52,980 --> 00:37:57,840
+water يعني يا إما بغمر إيدي تحت ماء بارد جاري لمدة
+
+510
+00:37:57,840 --> 00:38:02,680
+عشر دقائق أه بحط قطنة فيها ماء بارد على المكان زي
+
+511
+00:38:02,680 --> 00:38:07,200
+كمادة يعني لمدة عشر دقائق و cover with dry
+
+512
+00:38:07,200 --> 00:38:11,200
+dressing و بعدين بغطي المكان بشاشة إذا كان الجرح
+
+513
+00:38:11,200 --> 00:38:16,420
+severe أو الحرق severeيعني اطول اكيد بوجه الشخص
+
+514
+00:38:16,420 --> 00:38:20,260
+هذا اللي اتعرض للحرق ل ال medical treatment بحاوله
+
+515
+00:38:20,260 --> 00:38:27,000
+على العيادة او للطبيب بحيث انه يشوف علاج للجرح او
+
+516
+00:38:27,000 --> 00:38:32,520
+للحرق، تمام؟ال chemical burn of the skin لو انا
+
+517
+00:38:32,520 --> 00:38:37,320
+احترقت بفعل مادة كيميائية و ليس بفعل الحرارة ايش
+
+518
+00:38:37,320 --> 00:38:41,300
+بدي اعمل اول حاجة بدي اخسل المكان بكمية كبيرة من
+
+519
+00:38:41,300 --> 00:38:45,260
+الماء بعدين بدي اعمل ال neutralizing لالمادة
+
+520
+00:38:45,260 --> 00:38:48,920
+الكيميائية يعني انا لو احترقت ب acid بدي اعمل ال
+
+521
+00:38:48,920 --> 00:38:52,940
+neutralization يعني بدي اضيف ع مكان الحارق sodium
+
+522
+00:38:52,940 --> 00:38:55,920
+bicarbonate عشان ال sodium bicarbonate هي قاعدة
+
+523
+00:38:56,390 --> 00:38:59,710
+هتعادل ال acid وبالتالي تخفف من آثار ال damage
+
+524
+00:38:59,710 --> 00:39:05,110
+اللى ممكن يسببليها وإذا انا احترقت بمادة alkaline
+
+525
+00:39:05,110 --> 00:39:08,630
+بدي اضيف اعمل neutralization في boric acid powder
+
+526
+00:39:08,630 --> 00:39:14,850
+عشان هيك حكينا ان صندوق الاسعافات
+
+527
+00:39:14,850 --> 00:39:17,990
+الأولية لازم يكون في sodium bicarbonate و boric
+
+528
+00:39:17,990 --> 00:39:26,320
+acid تمام؟ طيبلو المادة كيميائية فاتت للعين إيش
+
+529
+00:39:26,320 --> 00:39:31,120
+بدي أعمل؟ حكينا قولنا إنه بدي أخسلها بالماء الجاري
+
+530
+00:39:31,120 --> 00:39:35,060
+لمدة ربيع ساعة و أعمل ال neutralize للمادة
+
+531
+00:39:35,060 --> 00:39:38,520
+الكيميائية هذه طبعا إذا كانت المادة الكيميا acid
+
+532
+00:39:38,520 --> 00:39:42,700
+اللي فاتت للعين بدي أستخدم عشان أعادلها و أقلل من
+
+533
+00:39:42,700 --> 00:39:47,280
+تأثيرها بدي أستخدم 5% Sodium Bicarbonate إذا كانت
+
+534
+00:39:47,280 --> 00:39:52,370
+alkaline بدي أستخدم 5% Acetic Acidimmediately seek
+
+535
+00:39:52,370 --> 00:39:56,210
+medical attention ع طول بعلم الطبيب او العيادة انه
+
+536
+00:39:56,210 --> 00:40:00,270
+صار في عندنا حادث شخص اتعرضت يعني اتقشف في عينه
+
+537
+00:40:00,270 --> 00:40:03,430
+مادة كيميائية و احنا عملنا له ال first aid و عليكم
+
+538
+00:40:03,430 --> 00:40:04,050
+اتكمله
+
+539
+00:40:06,680 --> 00:40:11,460
+swallowing an acid or base يعني ابتلاع مادة فيها
+
+540
+00:40:11,460 --> 00:40:15,740
+حمض أو قاعدة، إيش بدنا نعمل؟ أول حاجة immediately
+
+541
+00:40:15,740 --> 00:40:19,060
+rinse the mouth with water يعني أول حاجة بدي أغمر
+
+542
+00:40:19,060 --> 00:40:23,810
+الماء، أخليه مضمض يعني أكتر من مرةبالماء و بعدين
+
+543
+00:40:23,810 --> 00:40:28,970
+بروح اذا كان هو اطلع acid بدي اعمله neutralization
+
+544
+00:40:28,970 --> 00:40:33,710
+فيه باس يعني في 8% مغنيسيوم هيدروكسيد عشان هيك
+
+545
+00:40:33,710 --> 00:40:38,510
+المغنيسيوم هيدروكسيد من احد مكونات ال first aid
+
+546
+00:40:38,510 --> 00:40:43,950
+box تمام؟أو ممكن أخليه يشرب حليب milk of magnesia
+
+547
+00:40:43,950 --> 00:40:48,350
+تمام؟ عشان يعادل ال acid اللي هو ابطلعه إذا كان
+
+548
+00:40:48,350 --> 00:40:52,910
+الشخص ابطلع alkaline معناها لازم يعادله إياها ب
+
+549
+00:40:52,910 --> 00:40:57,710
+acid بعطيه lemon juice أو 5% acetic acid drink
+
+550
+00:40:57,710 --> 00:41:02,250
+three or four cups of water لازم يشرب ميه كتير
+
+551
+00:41:02,250 --> 00:41:05,330
+عشان يعمل dilution لهذا ال acid و ال base اللي ..
+
+552
+00:41:05,330 --> 00:41:09,210
+اللي ابطلعهو seek medical attention بوجهه للراعية
+
+553
+00:41:09,210 --> 00:41:13,070
+الصحية و بعلم الاعيادة او الدكتور في ان هو صار في
+
+554
+00:41:13,070 --> 00:41:18,310
+عندنا حادث لحتىيحطوه تحت المراقبة لما الشخص يبطلع
+
+555
+00:41:18,310 --> 00:41:21,770
+acid أو alkaline don't encourage vomiting
+
+556
+00:41:21,770 --> 00:41:27,010
+مابنخلوهوش يستفرغ ليش؟ لأنه هالجيت احنا النسيج تبع
+
+557
+00:41:27,010 --> 00:41:32,150
+البلعوم والحنجرة والمريق واتقصانا للمعدة هذا عبارة
+
+558
+00:41:32,150 --> 00:41:37,410
+.. النسيج هذا احترق أول مرة لما .. لما لمس ال acid
+
+559
+00:41:37,410 --> 00:41:40,930
+أو ال alkaline اللي احنا ابطلعناه تمام؟ لما نيجي
+
+560
+00:41:40,930 --> 00:41:45,100
+نعمل vomitingمعناها هيبدو يحترق مرة تانية يعني انا
+
+561
+00:41:45,100 --> 00:41:48,080
+هيك بزود ال damage كمية ال tissue اللي بيصيرلها
+
+562
+00:41:48,080 --> 00:41:51,460
+damage بسبب تعرضها لل acid و ال alkaline بتكون
+
+563
+00:41:51,460 --> 00:41:55,220
+كتير كبيرة اذا انا بقدر الشخص ما بفيده اذا هتخليته
+
+564
+00:41:55,220 --> 00:42:00,460
+يستفرغ فالافضل انه انا اخليه يغسل فمه نعادل المادة
+
+565
+00:42:00,460 --> 00:42:05,440
+اللي أخدها يخليه يشرب مي لحتى يعمل dilution و نحطه
+
+566
+00:42:05,440 --> 00:42:06,220
+تحت المراقبة
+
+567
+00:42:08,730 --> 00:42:12,730
+إذا ابتلع الشخص أي مادة سامة أخرى swallowing of
+
+568
+00:42:12,730 --> 00:42:18,050
+other poisonous chemical طبعا لازم يخسل ال .. فمه
+
+569
+00:42:18,050 --> 00:42:22,230
+بالماء جيدا و طبعا تبع ال .. المادة الكيميائية هذه
+
+570
+00:42:22,230 --> 00:42:25,030
+بستخدم ال antidote تبعها إيش يعني ال antidote يعني
+
+571
+00:42:25,030 --> 00:42:29,550
+literally neutralizing agent تبعها يعني إذا كانت
+
+572
+00:42:29,550 --> 00:42:33,830
+acid بنشوف هو إيش ممكن .. هاي هنا نرجع تاني إيش
+
+573
+00:42:33,830 --> 00:42:38,560
+ابتلع acid؟إيش بنخلق .. بنعمللهم ال neutralization
+
+574
+00:42:38,560 --> 00:42:44,020
+بيه مغنيسيم و hydroxide أو مغنيسية؟ إذا ابتلع مادة
+
+575
+00:42:44,020 --> 00:42:48,480
+alkaline بنعطيه lemon juice أو 5% acetic acid بس
+
+576
+00:42:48,480 --> 00:42:52,000
+هنا الفرق إيش؟ إن المادة سامة فإيش بدي أنا أخد
+
+577
+00:42:52,000 --> 00:42:56,760
+إجراءات تانية؟ على طول بدي أوديه على ال .. ال
+
+578
+00:42:56,760 --> 00:42:59,660
+chemical .. على ال physicalأو sorry يعني ال
+
+579
+00:42:59,660 --> 00:43:05,040
+physician على الدكتور لحتى يعني يعطوه علاج أو
+
+580
+00:43:05,040 --> 00:43:09,920
+يعطوه يعملوله غسيل المعدة أو أي إجراء لحتى يخلصه
+
+581
+00:43:09,920 --> 00:43:13,760
+من أثر المادة السامة هاي اللي ابتلعها Swallowing
+
+582
+00:43:13,760 --> 00:43:18,200
+of infected material إذا .. إذا ابتلع محلول فيه
+
+583
+00:43:18,200 --> 00:43:21,260
+بكتيريا أو فطريات أو أي مادة معضية infection ممكن
+
+584
+00:43:21,260 --> 00:43:25,780
+تعمله infection على طول بروح ب .. اوجهه لطبيب هو
+
+585
+00:43:25,780 --> 00:43:32,280
+اللي بيتعامل معاهتمام؟ إذا الشخص تعرض ل electric
+
+586
+00:43:32,280 --> 00:43:37,340
+shock يعني صدمة كهربائية بدي أ .. أول إشي بدي أطفل
+
+587
+00:43:37,340 --> 00:43:41,620
+كهربا from the mains من المصدر الرئيسي بدي أنزل
+
+588
+00:43:41,620 --> 00:43:46,020
+القاطع تمام؟ و إذا الشخص صار له collapse يعني أغمى
+
+589
+00:43:46,020 --> 00:43:51,150
+عليه أو فقد وعيه أو غيبعلى طول بدي أجيب .. بدي
+
+590
+00:43:51,150 --> 00:43:56,070
+أسأل الطبيب أو ممرض أو أي حد ييجي يسعفه و أتأكد
+
+591
+00:43:56,070 --> 00:44:02,290
+أنه مجرد تنفس عنده مفتوح أرفع رجلي لفوق أحاول أنه
+
+592
+00:44:02,290 --> 00:44:06,390
+أخل .. يعني أرفع رأس .. يعني أنزل رأس لتحت و رقبته
+
+593
+00:44:06,390 --> 00:44:10,500
+هيك خليها ..عالية شوية علشان انه اتأكد انه مجرد
+
+594
+00:44:10,500 --> 00:44:18,220
+تنافس عنده فاتح و طبعا حاول انه اذا كان الوضع صعب
+
+595
+00:44:18,220 --> 00:44:23,660
+على الآخر انه نعطيه انعاش قلب الرؤى.طيب كيف أنا
+
+596
+00:44:23,660 --> 00:44:26,320
+بدي حافظ على ال .. نظافة المختبر laboratory
+
+597
+00:44:26,320 --> 00:44:30,640
+hygiene ال hygiene يعني نظافة never eat, never
+
+598
+00:44:30,640 --> 00:44:33,720
+drink, never smoke يعني لا تاكلوا ولا تشربوا ولا
+
+599
+00:44:33,720 --> 00:44:36,820
+تدخنوا داخل المختبرات طبعا يعني الدنيا مش مستهلة
+
+600
+00:44:36,820 --> 00:44:42,720
+نجودي ندخن علشان حاجات رايحةnever apply cosmetics
+
+601
+00:44:42,720 --> 00:44:47,800
+ماحوتش ما .. ما .. ماسحق تجميل احنا حلوين رباني
+
+602
+00:44:47,800 --> 00:44:51,800
+never touch your face or mouth or eyes ماحاولش ان
+
+603
+00:44:51,800 --> 00:44:54,900
+انا ألمس .. أضل ألمس الوجه و الأيدين و ال eyes
+
+604
+00:44:54,900 --> 00:44:58,700
+بتلاحظوا احنا هنا نلقيت ما ..مع علامات ال .. او
+
+605
+00:44:58,700 --> 00:45:02,680
+sorry مع تحضيرات للوقاية من virus corona بنضلنا
+
+606
+00:45:02,680 --> 00:45:05,780
+نحكي انه يا جماعة الخير اغسلوا أدركوا منيه،
+
+607
+00:45:05,780 --> 00:45:09,600
+ماتلامسش وجهك، أنفك، عيناك، هذه برضه اجراءات
+
+608
+00:45:09,600 --> 00:45:13,160
+وقائية لكتير أمراض فبالتالي don't touch your face,
+
+609
+00:45:13,220 --> 00:45:17,280
+mouth, eyesملاحظ انهش الطالبة و هي لابسة ال gloves
+
+610
+00:45:17,280 --> 00:45:21,180
+بتنصدم هيك و بتتفاجأ هي و زملتها فعلى طول بتروح
+
+611
+00:45:21,180 --> 00:45:24,720
+اشحط ايدها على وجهها او على ثمها وهذه طبعا علامات
+
+612
+00:45:24,720 --> 00:45:30,340
+الدهشة فرجاء لا تندهشوا علشان انه ما تحافظوا على
+
+613
+00:45:30,340 --> 00:45:34,460
+ال hygiene تبعكم never suck pens or chew pencil
+
+614
+00:45:34,760 --> 00:45:39,260
+يعني ما تحاوليش أنك انت تفطي الأقلام الحبر أو
+
+615
+00:45:39,260 --> 00:45:43,600
+الرصاص في فمك و always wash your hands اغسل إيدك
+
+616
+00:45:43,600 --> 00:45:47,880
+باستمرار داخل .. اذا كنت في المختبر طبعا إذا كان
+
+617
+00:45:47,880 --> 00:45:52,800
+عندك allergyحساسية من أي مادة من .. في داخل
+
+618
+00:45:52,800 --> 00:45:56,240
+المختبر لازم تبلغي ال supervisor أو المدرس يعني
+
+619
+00:45:56,240 --> 00:45:59,300
+حاول .. لاحظتي أنك أنت بس تلبسي ال gloves بس
+
+620
+00:45:59,300 --> 00:46:03,480
+تتحسسي خبريني إذا شمتي مادة كيميائية معينة أو
+
+621
+00:46:03,480 --> 00:46:07,200
+حسيتي حالك اختنقتي أو تعبتي أو .. او اتحسستي منها
+
+622
+00:46:07,200 --> 00:46:10,660
+برضه كمان بلغيني لحتى أحاول أن أنا أبعدك عنها أو
+
+623
+00:46:10,660 --> 00:46:14,850
+أجيبلك بدائلطيب ال waste material حكينا أنه
+
+624
+00:46:14,850 --> 00:46:18,690
+الفضلات لازم كل جزء من الفضلات يكون موجود يعني ان
+
+625
+00:46:18,690 --> 00:46:23,850
+ال .. ان تخلص منه بطريقة safely تمامها dispose of
+
+626
+00:46:23,850 --> 00:46:28,210
+waste lab material safely، تخلص منه بأمان، كيف؟
+
+627
+00:46:28,210 --> 00:46:31,830
+يعني والله المواد السائلة بتخلص منها في .. داخل
+
+628
+00:46:31,830 --> 00:46:37,970
+زجاجاتsolvents and oils must be segregated لازم
+
+629
+00:46:37,970 --> 00:46:43,930
+انا اجمعها داخل ايه؟ bottles تمام؟ اذا كان ال ..
+
+630
+00:46:43,930 --> 00:46:49,790
+المواد مشعة بدي احطها في صناديق و اتلفها بطريقة
+
+631
+00:46:49,790 --> 00:46:53,990
+معينة اذا كانت infectant ممكن تعمل .. تنقل عدوة
+
+632
+00:46:53,990 --> 00:46:59,100
+لازم اعقمها و بعدين اتلفهازي إذا كانت مواعد حادة
+
+633
+00:46:59,100 --> 00:47:03,720
+لازم أحطها في ال sharp box فبالتالي كل إشي لازم
+
+634
+00:47:03,720 --> 00:47:08,720
+يطلق بالطريقة الصحيحة لحتى أتخلص منه بأقل الأضرار
+
+635
+00:47:08,720 --> 00:47:18,300
+و طبعا مافعش أنا أتخلص منه بطريقة غير مسئولة أو
+
+636
+00:47:18,300 --> 00:47:23,160
+بطريقة بدون تعليماتدايما انا المدرس احكيلكم حطي ..
+
+637
+00:47:23,160 --> 00:47:25,940
+اتخلصي من ال needle داخل ال sharp box على طول
+
+638
+00:47:25,940 --> 00:47:29,000
+بتخلصي .. بتخلصي منها بال sharp box حكيتلكم انه
+
+639
+00:47:29,000 --> 00:47:32,680
+هيكون في تعليمات لتعامل مع ال waste material في كل
+
+640
+00:47:32,680 --> 00:47:38,180
+تجربة و في كل مختبر احنا بن .. بنجريه لحتى تكون ..
+
+641
+00:47:38,180 --> 00:47:43,440
+تصير بعد هيك عندكم دراية و متدربين منيح انه كيف و
+
+642
+00:47:43,440 --> 00:47:47,760
+وين تتلي في كل مادة بتستخدميها طبعا ..
+
+643
+00:47:50,550 --> 00:47:54,570
+you will need to attend training courses اذا كان
+
+644
+00:47:54,570 --> 00:47:58,590
+انت .. بتحتاج .. بتحتاجي لتدريد اذا كان انت بدك
+
+645
+00:47:58,590 --> 00:48:06,090
+تدومي في .. في ساعات outside the normal hours يعني
+
+646
+00:48:06,090 --> 00:48:09,810
+مثلا في الشفتات الليلية و المساء هذا .. هذا لما
+
+647
+00:48:09,810 --> 00:48:14,420
+تختار .. يعنيتتخرجوا و تشتغلوا فلازم يكون عندك
+
+648
+00:48:14,420 --> 00:48:19,100
+تدريب لإنه طبعا يعني مثلا ال accident في النهار في
+
+649
+00:48:19,100 --> 00:48:23,440
+المنشأة فيها موظفين كتير و فيها ناس ممكن ينقذوكي
+
+650
+00:48:23,440 --> 00:48:27,040
+مش زي لما يحدث معاكي accident في الليل و الناس ..
+
+651
+00:48:27,040 --> 00:48:30,260
+و الموظفين كلهم في بيوتهم و انت بس اللي مناوبة
+
+652
+00:48:30,260 --> 00:48:32,860
+فلازم برضه كمان يعني يكون في عندك تدريب كيف
+
+653
+00:48:32,860 --> 00:48:35,920
+تتعاملي معاه ده أصلا مش كل الشغل بينشغل في الليل
+
+654
+00:48:35,920 --> 00:48:41,160
+يعنيشفتات المسائق بيقللوا فيها نسبة الشغل لحتى
+
+655
+00:48:41,160 --> 00:48:45,580
+يقللوا من الأضرار اللي ممكن تلحق بالأشخاص و طبعا
+
+656
+00:48:45,580 --> 00:48:48,980
+هاي هنا بيحكيلك most experimental work is not
+
+657
+00:48:48,980 --> 00:48:52,100
+permitted يعني معظم الشغل مابيكونش مسموح أنك
+
+658
+00:48:52,100 --> 00:48:56,700
+تشتغليه في الليل و طبعا أكيد مدير المختبر بيشرحلك
+
+659
+00:48:56,700 --> 00:49:00,300
+بالتفصيل إيش مسموح و إيش مش مسموح، إيش عليك و إيش
+
+660
+00:49:00,300 --> 00:49:07,890
+إلكداخل المختبر تمام حكى هنا انه ماتشتغليش don't
+
+661
+00:49:07,890 --> 00:49:11,690
+carry out a new or unfamiliar procedure يعني اي
+
+662
+00:49:11,690 --> 00:49:16,650
+تجربة غريبة ومش مألوفة تشتغليهاش اقتلي الشخص
+
+663
+00:49:16,650 --> 00:49:19,770
+الكيميائي اللي جواكي قبل تفوت المختبر don't guess
+
+664
+00:49:19,770 --> 00:49:25,990
+ماتخمنيش ماتفكريش ماتسرحيش ولا تشطحي انه والله لو
+
+665
+00:49:25,990 --> 00:49:28,960
+ضفت المادة هاي على المادة هاي إيش ممكن أحصليعني مش
+
+666
+00:49:28,960 --> 00:49:32,560
+هنحصل على هالإنجاز الكيميائي الكبير جدما أن أحنا
+
+667
+00:49:32,560 --> 00:49:37,980
+ممكن نعرض حالنا لخطر حكينا هذه ال slide شرحناها
+
+668
+00:49:37,980 --> 00:49:43,380
+قولنا إنه مش لازم أنا أطلق حس الدعابة داخل المختبر
+
+669
+00:49:43,380 --> 00:49:48,340
+و أعمل مجالب زملائي عشان ما أعرض نفسي أو أعرضهم
+
+670
+00:49:48,340 --> 00:49:52,400
+للأذى و برضه كمان في عندنا مخازن بتكون مخزن عليها
+
+671
+00:49:52,400 --> 00:49:56,590
+المواد الكيميائيةممنوع تدخليها إلا بإذن و برضه
+
+672
+00:49:56,590 --> 00:49:59,770
+كمان ممنوع تاخدي أي مادة كيميائية منها برضه إلا
+
+673
+00:49:59,770 --> 00:50:06,790
+بإذن و تلمسي أو تستخدمي أي مادة كيميائية إلا إذا
+
+674
+00:50:06,790 --> 00:50:09,370
+أنا أعطيتك تعليمات فيها و غالبا إنه بيكون اللي
+
+675
+00:50:09,370 --> 00:50:14,190
+متعامل مع المخزن أنا و الفني المختبر حتى كمان أنا
+
+676
+00:50:14,190 --> 00:50:17,810
+بيكون ال access تبعي على هذا المخزن قليل يعني
+
+677
+00:50:17,810 --> 00:50:21,690
+دخولي للمخزن بيكون قليل جداغالبا بيكون في عنا موظف
+
+678
+00:50:21,690 --> 00:50:25,030
+فني مختبر انا دايما بكتبله ورقة بقاش المواد اللي
+
+679
+00:50:25,030 --> 00:50:28,270
+بديها فهو بطلع ليها من المخزن و هيك انا بكون حاميت
+
+680
+00:50:28,270 --> 00:50:31,390
+نفسي فانه كمان برضه الطالبات مش لازم يغامروا
+
+681
+00:50:31,390 --> 00:50:36,910
+يفوتوا للمخزن و يعرضوا حالهم للخطرإذن بدنا نتذكر
+
+682
+00:50:36,910 --> 00:50:40,510
+إنه لازم stay at your work station لازم نضلنا في
+
+683
+00:50:40,510 --> 00:50:44,170
+مكان عملنا طول و عندنا شغال maintain أكلين و .. و
+
+684
+00:50:44,170 --> 00:50:48,910
+a clean work area لازم يكون في عندى مكان عمل نظيف
+
+685
+00:50:48,910 --> 00:50:52,810
+أحافظ عليه نظيف read and follow all directions كل
+
+686
+00:50:52,810 --> 00:51:00,030
+التعليمات أقرأها جيدا و أتبعهاأى spell انسكاب حادث
+
+687
+00:51:00,030 --> 00:51:03,790
+أو injury اتعرضة اتلاقلها او اتعرضلها زملائى لازم
+
+688
+00:51:03,790 --> 00:51:09,830
+تصيرلها reported يعني تكتب تسجل و لازم ا اعمل
+
+689
+00:51:09,830 --> 00:51:14,090
+cleaning انظف او ارفع كل ال equipment بعد ما اخلص
+
+690
+00:51:14,090 --> 00:51:18,190
+من استخدامها و اتخلص من ال waste بالشكل المناسب و
+
+691
+00:51:18,190 --> 00:51:21,630
+هيك بنكون خلصنا علامات السلامة و الأمان اللى داخل
+
+692
+00:51:21,630 --> 00:51:27,450
+المختبر و خلصنا محاضرتنا لليوم طبعاحاولوا أنكم
+
+693
+00:51:27,450 --> 00:51:31,370
+تسمعوها مرة مرتين و تلاتة و مش غلط أنكم تعملوا
+
+694
+00:51:31,370 --> 00:51:35,830
+تلخيص بأهم المعلومات اللي داخلة داخل ال .. اللي
+
+695
+00:51:35,830 --> 00:51:40,170
+يعني اللي داخلة داخل ال chapter هذا و حتى أنه تسهل
+
+696
+00:51:40,170 --> 00:51:45,990
+رجوعكم إليها و تسهل دراستها بشدد يعني أو ب .. ب ..
+
+697
+00:51:45,990 --> 00:51:52,830
+بشدد عليكم أو يعنيأنوه .. أنوه .. الكلمات العربية
+
+698
+00:51:52,830 --> 00:51:57,470
+أحيانا بتضيع مني أنه يعني المواد الكيميائية هذه
+
+699
+00:51:57,470 --> 00:52:00,970
+اللي أنا بدأ أستخدمها لل .. لل neutralization مهمة
+
+700
+00:52:00,970 --> 00:52:04,130
+يعني مثلا في حالة الحرق ال .. ب acid إيش أستخدم له
+
+701
+00:52:04,130 --> 00:52:07,130
+neutralization؟ في حالة ال alkaline إيش أستخدم؟ في
+
+702
+00:52:07,130 --> 00:52:10,210
+حالة الإبتلاء؟ في حالة المادة اللي بت .. بتفوت جوا
+
+703
+00:52:10,210 --> 00:52:13,050
+العين؟ إذا كانت acid أو base إيش أستخدملها؟ ال
+
+704
+00:52:13,050 --> 00:52:16,570
+antidote تبعها أو ال neutralization تبعها تمام؟
+
+705
+00:52:16,570 --> 00:52:19,770
+كلمة antidote أو neutralizing agent نفس المعنى
+
+706
+00:52:20,150 --> 00:52:23,830
+فالمهمين هدولة تحفظوهم حلو لو أنكوا لخصتهم على
+
+707
+00:52:23,830 --> 00:52:28,170
+ورقة واستخدمتوهم يعني راجعتوهم خلال كل دراستكم أو
+
+708
+00:52:28,170 --> 00:52:32,120
+كل ما يكون عليك ال quiz أو إشي ترجعيلهم مش هقلطهيك
+
+709
+00:52:32,120 --> 00:52:36,040
+بنكون خلصنا محاضرتنا لليوم، يعطيكم ألف عافية و
+
+710
+00:52:36,040 --> 00:52:39,340
+شكرا لكم، أي استفسار، أي سؤال بالمحاضرة هذه و
+
+711
+00:52:39,340 --> 00:52:42,600
+المحاضرة السابقة ان شاء الله نلقاكم يوم السبت
+
+712
+00:52:42,600 --> 00:52:47,100
+الساعة ستة على ال zoom لحتى نلتقي منكم أو نستقبل
+
+713
+00:52:47,100 --> 00:52:52,100
+منكم الأسئلة و ناخد إجابات عليها أو يصير حتى نقاش
+
+714
+00:52:52,100 --> 00:52:56,260
+حولها، ممكن أضربلكم أمثلة لحتى أقربلكم الفهمممكن
+
+715
+00:52:56,260 --> 00:52:58,960
+البنت مش فاهمة slide أرجع أشرحلها إياها نفتح ال
+
+716
+00:52:58,960 --> 00:53:03,480
+slide و نرجع نقرأها و نشرحها ممكن أنا يكون عندي
+
+717
+00:53:03,480 --> 00:53:08,400
+أسئلة أو أنتم يكون عندكم أسئلة ف الساعة اللي بناخد
+
+718
+00:53:08,400 --> 00:53:12,180
+فيها مناقشة ال zoom مهمة جدا يا بنات و رجاء ما
+
+719
+00:53:12,180 --> 00:53:15,520
+اتفرطوا فيها و زي ما حكيتلكم إذا حسيت ما فيها ال
+
+720
+00:53:15,520 --> 00:53:20,240
+design رح يكون عليها حضور و غياب هيك أنا بكون خلصت
+
+721
+00:53:20,240 --> 00:53:23,100
+و موفقين بإذن الله شكرا لكم
+
diff --git a/PL9fwy3NUQKwY9jl8xw-bWkVZkWcGGyspd/fqKeQtgENUo_raw.json b/PL9fwy3NUQKwY9jl8xw-bWkVZkWcGGyspd/fqKeQtgENUo_raw.json
new file mode 100644
index 0000000000000000000000000000000000000000..2df872e1472a40ebbe0016aa5c5f095d4066d329
--- /dev/null
+++ b/PL9fwy3NUQKwY9jl8xw-bWkVZkWcGGyspd/fqKeQtgENUo_raw.json
@@ -0,0 +1 @@
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"فبالتالي لازم يكون مشار إلى الأسفل. Never try to touch falling sharp instrument. إذا انكسر أدوات حادة زجاجية مثلا وجعة شفرة أو أدوات حادة، وجع بيكر وانكسر، إشي زجاجي انكسر، مش لازم أنا أروح أنمسه بإيدي نهائيا علشان ما أجرح نفسي.", "tokens": [5172, 3555, 6027, 2655, 6027, 1829, 5296, 31377, 2304, 7251, 30544, 37893, 9640, 30731, 16247, 3794, 5172, 1211, 13, 7344, 853, 281, 2557, 7440, 8199, 7198, 13, 11933, 15730, 16472, 4117, 3794, 2288, 5551, 3215, 2407, 9307, 11331, 18513, 3660, 30767, 7435, 26108, 10632, 50113, 15040, 49610, 27884, 13412, 5172, 25720, 34051, 5551, 3215, 2407, 9307, 11331, 18513, 3660, 12399, 49610, 3615, 4724, 1829, 37983, 4032, 7649, 4117, 3794, 2288, 12399, 11933, 8592, 1829, 30767, 7435, 26108, 1829, 16472, 4117, 3794, 2288, 12399, 37893, 5296, 31377, 2304, 41850, 5551, 32887, 5016, 14739, 2304, 3794, 3224, 4724, 28814, 1829, 16254, 8717, 3224, 16373, 25528, 11203, 8592, 7649, 19446, 5551, 7435, 2288, 5016, 8717, 36178, 1829, 13], "avg_logprob": -0.11321390304585983, "compression_ratio": 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3184.06, "word": " أو", "probability": 0.83642578125}, {"start": 3184.06, "end": 3184.3, "word": " أنتم", "probability": 0.587646484375}, {"start": 3184.3, "end": 3184.56, "word": " يكون", "probability": 0.981689453125}, {"start": 3184.56, "end": 3184.98, "word": " عندكم", "probability": 0.90576171875}, {"start": 3184.98, "end": 3185.48, "word": " أسئلة", "probability": 0.9949951171875}, {"start": 3185.48, "end": 3186.12, "word": " ف", "probability": 0.9404296875}, {"start": 3186.12, "end": 3187.24, "word": " الساعة", "probability": 0.777099609375}, {"start": 3187.24, "end": 3187.7, "word": " اللي", "probability": 0.7744140625}, {"start": 3187.7, "end": 3188.4, "word": " بناخد", "probability": 0.919921875}, {"start": 3188.4, "end": 3188.58, "word": " فيها", "probability": 0.9833984375}, {"start": 3188.58, "end": 3189.24, "word": " مناقشة", "probability": 0.97509765625}, {"start": 3189.24, "end": 3189.4, "word": " ال", "probability": 0.880859375}, {"start": 3189.4, "end": 3189.64, 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"probability": 0.982421875}, {"start": 3193.46, "end": 3194.12, "word": " حكيتلكم", "probability": 0.935546875}, {"start": 3194.12, "end": 3194.6, "word": " إذا", "probability": 0.9287109375}, {"start": 3194.6, "end": 3195.0, "word": " حسيت", "probability": 0.8846028645833334}, {"start": 3195.0, "end": 3195.12, "word": " ما", "probability": 0.896484375}, {"start": 3195.12, "end": 3195.38, "word": " فيها", "probability": 0.783935546875}, {"start": 3195.38, "end": 3195.52, "word": " ال", "probability": 0.87890625}, {"start": 3195.52, "end": 3195.8, "word": " design", "probability": 0.318115234375}, {"start": 3195.8, "end": 3196.06, "word": " رح", "probability": 0.825927734375}, {"start": 3196.06, "end": 3196.32, "word": " يكون", "probability": 0.99072265625}, {"start": 3196.32, "end": 3196.82, "word": " عليها", "probability": 0.979736328125}, {"start": 3196.82, "end": 3197.2, "word": " حضور", "probability": 0.9832356770833334}, {"start": 3197.2, "end": 3197.32, "word": " و", 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\ No newline at end of file
diff --git a/PL9fwy3NUQKwY9jl8xw-bWkVZkWcGGyspd/fqKeQtgENUo_raw.srt b/PL9fwy3NUQKwY9jl8xw-bWkVZkWcGGyspd/fqKeQtgENUo_raw.srt
new file mode 100644
index 0000000000000000000000000000000000000000..102f12ee58b239a2710bead58989871d4f4e8c57
--- /dev/null
+++ b/PL9fwy3NUQKwY9jl8xw-bWkVZkWcGGyspd/fqKeQtgENUo_raw.srt
@@ -0,0 +1,2884 @@
+1
+00:00:10,530 --> 00:00:14,590
+السلام عليكم ورحمة الله وبركاته طلبات العزيزات
+
+2
+00:00:14,590 --> 00:00:19,590
+اليوم ان شاء الله رح نكمل موضوعنا المفروض يعني هو
+
+3
+00:00:19,590 --> 00:00:26,370
+يعني يندرج تحت العملي عن ال laboratory safety عن
+
+4
+00:00:26,370 --> 00:00:32,030
+قواعد السلام والأمانةالصراحة المحاضرة اليوم فيها
+
+5
+00:00:32,030 --> 00:00:38,150
+يعني ملاحظات كتير فيها كلام كتير ولكن ال .. الاشي
+
+6
+00:00:38,150 --> 00:00:45,330
+مفهوم يعني و هو ممكن انتوا يعني تحسي حالك زهجتي من
+
+7
+00:00:45,330 --> 00:00:49,030
+الكلام بس في النهاية انت يعني انا .. انا بتخيل انه
+
+8
+00:00:49,030 --> 00:00:54,530
+انك تسمعي لمرة واحدة او مرتينراح يرسخ يعني هو مش
+
+9
+00:00:54,530 --> 00:00:58,690
+صعب بس إنما هو كلام و حتى هيكون مكرر يعني في جزئية
+
+10
+00:00:58,690 --> 00:01:04,330
+منيحة يعني أنه شرحناها أو غطيناها خلال المحاضرة
+
+11
+00:01:04,330 --> 00:01:09,130
+النظرية السابقة فالكلام يعني هيكون في جزء جديد
+
+12
+00:01:09,130 --> 00:01:13,210
+عليكم في جزء اسمعته قبل هيك فأتمنى إن شاء الله
+
+13
+00:01:13,210 --> 00:01:16,950
+يعني تكون كل الأمور تمام و كلها باضحةطبعا احنا
+
+14
+00:01:16,950 --> 00:01:21,330
+حكينا في ال .. في المختبر طبعا أنا ليش بحافظة على
+
+15
+00:01:21,330 --> 00:01:25,710
+قواعد السلامة و الأمان علشان ال .. ال safe working
+
+16
+00:01:25,710 --> 00:01:30,070
+الشغل الأمن بيحمي مين؟ بيحمي نفسي و ال other lab
+
+17
+00:01:30,070 --> 00:01:34,430
+workers زملائي و ال cleaners ال .. الناس عمال
+
+18
+00:01:34,430 --> 00:01:37,870
+النظافة اللي بيشرفوا على نظافة المكان ال visitors
+
+19
+00:01:37,870 --> 00:01:42,850
+زوار المكان و يعني المرضى اللي بيرتعدوا المكانو
+
+20
+00:01:42,850 --> 00:01:46,610
+أيضا الشغل تبعي طالما أنا إذا كنت بشتغل شغل safe
+
+21
+00:01:46,610 --> 00:01:51,330
+شغل آمن إذا هيكون شغلي صحيح تمام و هحصل على نتائج
+
+22
+00:01:51,330 --> 00:01:56,890
+صحيحة تفيد في ال diagnosis حكينا
+
+23
+00:01:56,890 --> 00:02:00,570
+.. هذا .. هذا كلام مععد حكينا قبل هيك أنه لازم
+
+24
+00:02:00,570 --> 00:02:04,930
+أحافظ على ارتداء ال lab coat تمام و لازم ال long
+
+25
+00:02:04,930 --> 00:02:09,250
+hair يكون مرفوع الشعر اللي طويل .. sorry
+
+26
+00:02:12,690 --> 00:02:18,330
+الشعر لازم يكون مرفوع و طبعا ما نلبسش ملابس واسعة
+
+27
+00:02:18,330 --> 00:02:22,230
+و لازم ال shoes يكون closed مش يكون مفتوح ال
+
+28
+00:02:22,230 --> 00:02:26,750
+sandals و الأشياء المفتوحة هاي نروح .. نحاول أنه
+
+29
+00:02:26,750 --> 00:02:30,840
+ما ناخدهاتمام؟ و بالنسبة كمان ايضا لازم ارتدي ل
+
+30
+00:02:30,840 --> 00:02:33,260
+gloves، ل gloves انه انا في عندي نوعين من ال
+
+31
+00:02:33,260 --> 00:02:36,520
+gloves، في ال gloves اللي هي مصنوعة من ال latex
+
+32
+00:02:36,520 --> 00:02:40,540
+اللي زي ال nylon هاد، هاد اللي احنا بنستخدمه للشغل
+
+33
+00:02:40,540 --> 00:02:44,720
+طبعنا، طبعا في منه أنواع و في منه درجات حتى في
+
+34
+00:02:44,720 --> 00:02:48,960
+نسبة ال protection يعني في بيحمي أكتر في بيحمي أقل
+
+35
+00:02:49,170 --> 00:02:55,070
+فرح ناخدهم طبعا بالتفصيل لقدام شوية و أيضا في ال
+
+36
+00:02:55,070 --> 00:02:59,890
+gloves السميك اللي مصنوع من النسيج أو القماش اللي
+
+37
+00:02:59,890 --> 00:03:07,210
+هو للتعامل مع الأشياء الساخنةطبعا في قاعدة بتحكيلك
+
+38
+00:03:07,210 --> 00:03:09,610
+remove your gloves before using an instrument,
+
+39
+00:03:09,830 --> 00:03:12,490
+telephone and leaving the laboratory طبعا قبل أن
+
+40
+00:03:12,490 --> 00:03:16,830
+أستخدم أي جهاز و أنا بتعامل مع العين أو المحاليل و
+
+41
+00:03:16,830 --> 00:03:19,410
+الأدوات لازم أكون لابسة gloves لما أجد أستخدم جهاز
+
+42
+00:03:19,410 --> 00:03:22,510
+أو حتى ال telephone تبعي أو ألمس أغراضي أو أغادر
+
+43
+00:03:22,510 --> 00:03:25,890
+من المختبر لازم أكون تخلص من ال gloves و أتلفتها
+
+44
+00:03:25,890 --> 00:03:30,420
+علشان ما أنشر ال contaminationeverywhere طبعا لو
+
+45
+00:03:30,420 --> 00:03:34,100
+انسكب اي مادة كيميائية على الجلب لازم انا اغسلها
+
+46
+00:03:34,100 --> 00:03:38,420
+بالماء لمدة ربع ساعة لازم اغسل يديا قبل و بعد
+
+47
+00:03:38,420 --> 00:03:44,140
+المختبر و ايضا ال .. ال .. ال .. هو انا مش عارفة
+
+48
+00:03:44,140 --> 00:03:51,710
+من اللي عامله عرض فهو العرض يعنيغريب، طيب و بحكيلك
+
+49
+00:03:51,710 --> 00:03:55,450
+هنا handle a glassware sharp tool and heated
+
+50
+00:03:55,450 --> 00:04:00,030
+containers carefully، اتعاملي مع المواد الزجاجية،
+
+51
+00:04:00,030 --> 00:04:04,690
+الأدوات الحادة و الأدوات الساخنة بحذر
+
+52
+00:04:09,980 --> 00:04:13,420
+بنتعامل مع .. بنستخدم ال safety goggles النضارات
+
+53
+00:04:13,420 --> 00:04:17,520
+لو انا بستخدم مادة كيميائية لها ال .. يعني لها
+
+54
+00:04:17,520 --> 00:04:25,220
+بمتطاير او رذاذ متطاير او حتى يعني depress اللي هي
+
+55
+00:04:25,220 --> 00:04:30,040
+يعني بقايا متطايرة ممكن اتلامسها عيوني انا بتخلص
+
+56
+00:04:30,040 --> 00:04:37,940
+منها اذا كان طبعا اي حد بيرتدي عدساتلاصقة لازم
+
+57
+00:04:37,940 --> 00:04:43,120
+يخبر الدكتور و أيضا يعني يخبر المدرس اللي موجود في
+
+58
+00:04:43,120 --> 00:04:48,560
+المختبر و أيضا في حالة ال .. ال emergency لو يعني
+
+59
+00:04:48,560 --> 00:04:51,860
+في حالة الطوارئ لو أي إشي أي chemical دخلت إلى
+
+60
+00:04:51,860 --> 00:04:55,240
+العين لازم أنا أخسلها من خلال ال washer تبع العين
+
+61
+00:04:55,240 --> 00:04:58,380
+لمدة ربع ساعة و احنا حكينا عن ال washer هذه
+
+62
+00:04:58,380 --> 00:05:01,880
+المحاضرة الماضيةطبعا إيش الأخطار اللي ممكن تقابلني
+
+63
+00:05:01,880 --> 00:05:06,000
+داخل المختبر؟ اللي هي خطر حدوث حرائق، خطر إيه يعني
+
+64
+00:05:06,000 --> 00:05:10,520
+كسر لمادة زجاجية، الأدوات الحادة، خطر انسكاب أي
+
+65
+00:05:10,520 --> 00:05:16,960
+مادة كيميائية، خطر الغاز و السوائل المضغوطة و خطر
+
+66
+00:05:16,960 --> 00:05:21,180
+الحرارة و البرودة، طالما أن الحرارة العالية و
+
+67
+00:05:21,180 --> 00:05:25,200
+البرودة العالية ممكن تسبب حروق للجسمخطر المواد
+
+68
+00:05:25,200 --> 00:05:28,080
+الكيميائية والمواد البيولوجية زي الفيروسات
+
+69
+00:05:28,080 --> 00:05:30,780
+والبكتيريا والفطريات والطفاليات اللي بتكون موجودة
+
+70
+00:05:30,780 --> 00:05:34,920
+داخل المختبر الإشعاع طيب و أي أنواع أخرى من
+
+71
+00:05:34,920 --> 00:05:38,920
+المخاطر لو كان في أنتي .. أنا كيف بدي أتجنب حدوث
+
+72
+00:05:38,920 --> 00:05:42,760
+حرائق أول حاجة المادة اللي بسميها flammable إيش
+
+73
+00:05:42,760 --> 00:05:47,600
+يعني flammable يعني مادة حارقة تمام أو مشتعلة طبعا
+
+74
+00:05:47,600 --> 00:05:50,800
+بدي أ .. لازم يكون في عندي منها في المختبر كميات
+
+75
+00:05:50,800 --> 00:05:55,620
+قليلة مافعش أخزنها بكميات كتير كبيرةتمام store in
+
+76
+00:05:55,620 --> 00:05:59,300
+special storage cabinet طبعا بحطها في كابينات خاصة
+
+77
+00:05:59,300 --> 00:06:04,220
+بيبقى محافظة على برودتها انها يعني موفرالي درجة
+
+78
+00:06:04,220 --> 00:06:08,980
+حرارة .. حرارة باردة عساس ان هاي المادة ما تشتعل و
+
+79
+00:06:08,980 --> 00:06:11,820
+طبعا لازم استخدم temperature controlled heating
+
+80
+00:06:11,820 --> 00:06:16,260
+source يعني .. يعني يكون في عندي قياس مستمر ل ..
+
+81
+00:06:16,290 --> 00:06:21,790
+الدرجة الحرارة داخل هاي ال cabinet ع أساس انه ما
+
+82
+00:06:21,790 --> 00:06:26,650
+يكون في عندى ارتفاع لدرجات الحرارة ممكن يسبب
+
+83
+00:06:26,650 --> 00:06:30,830
+اشتعالها حتى لما بدي اتعامل مع هاي المادة اللى
+
+84
+00:06:30,830 --> 00:06:34,750
+بنسميها فيلم بالمشتعلة زى الكحول مثلالازم بدي
+
+85
+00:06:34,750 --> 00:06:38,150
+أسخنها بستخ .. بسخنها باستخدام ال water bath بدل
+
+86
+00:06:38,150 --> 00:06:41,810
+ما أحطها مباشرة على النار وتشتعل لأ بستخدمها ..
+
+87
+00:06:41,810 --> 00:06:44,430
+بسخنها باستخدام water bath في كتير من المواد
+
+88
+00:06:44,430 --> 00:06:49,870
+بنسميها flammable مش بس كحول أحيانا ال ether ال ..
+
+89
+00:06:49,870 --> 00:06:55,950
+يعني ..ال Acetone كتير مواد هنتعامل معها بعض ال
+
+90
+00:06:55,950 --> 00:07:00,510
+acids هنتعامل .. نتعامل معهم بيعطينا تحدير أنه هذه
+
+91
+00:07:00,510 --> 00:07:03,230
+المادة flammable لما بدي سخنها بسخنها باستخدام
+
+92
+00:07:03,230 --> 00:07:06,670
+water bath يعني حمام مائي أفضل من ما أعرضها لدرجة
+
+93
+00:07:06,670 --> 00:07:10,810
+الحرارة مباشرةو بتتخزن ب cabinet و بنستخدم منها
+
+94
+00:07:10,810 --> 00:07:14,350
+كميات قليلة تمام؟ بتكون عندي بدل ما أنا أستخدم
+
+95
+00:07:14,350 --> 00:07:17,970
+tank داخل المختبر من الكحول مثلا لأ أنا ب .. ب ..
+
+96
+00:07:17,970 --> 00:07:21,950
+بخليها في المخزن و بجيب على المختبر bottles على جد
+
+97
+00:07:21,950 --> 00:07:26,490
+استخدامي و حاجتي make sure that you know what to
+
+98
+00:07:26,490 --> 00:07:30,510
+do طبعا إذا حدث حرائق لازم تكون تعرفي تعملي القاتل
+
+99
+00:07:30,510 --> 00:07:34,740
+if you have a fireif you hear a fire alarm إذا انت
+
+100
+00:07:34,740 --> 00:07:39,940
+كان في عندك حريق أو كان في عندك انذار لحدوث حريق
+
+101
+00:07:39,940 --> 00:07:43,620
+لازم تكوني بتعرفي إيش بدك تعملي، كيف بدك تتصرفي،
+
+102
+00:07:43,620 --> 00:07:48,160
+كيف يعني؟ يعني مثلا بتعرفي مكان ال fire .. ال fire
+
+103
+00:07:48,160 --> 00:07:51,580
+fighting equipmentتعرف في مكان أدوات اللي هي
+
+104
+00:07:51,580 --> 00:07:55,860
+محاربة الحدوث حرائق زي ال blanket البطامية أعرف
+
+105
+00:07:55,860 --> 00:08:01,400
+وان موجودة داخل المختبر النبوب .. النبوبة لإطفاء
+
+106
+00:08:01,400 --> 00:08:06,220
+الحرائق أيضا كمان أعرف وان موجودة في عندي أدوات
+
+107
+00:08:06,220 --> 00:08:09,940
+برضه بستخدمها لإطفاء أنواع كتير من الحرائق زي
+
+108
+00:08:09,940 --> 00:08:15,080
+الرمل و ال .. و أنابيب مي و أشياء هذه ده كنت عارفة
+
+109
+00:08:15,080 --> 00:08:15,900
+وان موجودة
+
+110
+00:08:19,070 --> 00:08:23,590
+و أيضا غير أن أنا بدأ أكون عارفة وين موجودة، بدي
+
+111
+00:08:23,590 --> 00:08:27,270
+أكون عارفة مثلا مخرج التوارق من وين، كل مختبر
+
+112
+00:08:27,270 --> 00:08:31,630
+بيكون إيه له في الخارج مخرج .. مخرج توارق موجود
+
+113
+00:08:31,630 --> 00:08:37,890
+فيه، فبالتالي لازم نكون بنعرف كيف نستخدمه تمام؟
+
+114
+00:08:37,890 --> 00:08:42,490
+هذا بالنسبالي الجملة اللي معناها know the location
+
+115
+00:08:42,490 --> 00:08:47,950
+of firefighting equipmentطيب طبعا في عندي كل نوع
+
+116
+00:08:47,950 --> 00:08:52,070
+حرائق أنا بستخدملها مادة لإطفاءها يعني مثلا ال
+
+117
+00:08:52,070 --> 00:08:55,250
+bucket of water بيكون في عندي بكتات للماء مع ..
+
+118
+00:08:55,250 --> 00:08:58,970
+معبقة بالماء طبعا ها دي بتستخدم to extinguish
+
+119
+00:08:58,970 --> 00:09:03,870
+paper and wood fire ل .. لحتى أطفئ الحرائق اللي
+
+120
+00:09:03,870 --> 00:09:07,270
+ناتج من اشتعال ورق أو خشب لو بنش خشب .. طبعا ..
+
+121
+00:09:07,710 --> 00:09:10,930
+يعني شبكت فيه نار و هي بدأ .. بلش يحترق أو أوراق
+
+122
+00:09:10,930 --> 00:09:14,950
+على طول بروح أش بجيب ال buckets of water و بفتحها
+
+123
+00:09:14,950 --> 00:09:22,390
+و بكبها عليها تمام؟ فبالتالي ال .. يعني الميها هي
+
+124
+00:09:22,390 --> 00:09:27,270
+مستحيل أستخدمها لحتى أطف حرائق ناتج عن الكهرة بل
+
+125
+00:09:27,270 --> 00:09:30,170
+أنها هي هتسبب انفجار يعني أو هت .. هتعمل mass
+
+126
+00:09:30,170 --> 00:09:34,970
+كهربائي فبالتالي يعني كل مادة تستخدم في نوع من
+
+127
+00:09:34,970 --> 00:09:38,440
+أنواع الحرائقال bucket of sand بكتات بيكون فيها
+
+128
+00:09:38,440 --> 00:09:43,660
+رمل طبعا هذه الرمل أو حتى تربة جافة dry soil طبعا
+
+129
+00:09:43,660 --> 00:09:52,300
+تستخدم للحرائق اللي ناتجة من اش السوائل
+
+130
+00:09:52,300 --> 00:09:57,080
+المشتعلة يعني لو كحولطبعا سائل هذا الكحول اشتعل
+
+131
+00:09:57,080 --> 00:10:00,620
+على طول بدي اروح على .. يعني انكب على البنش و
+
+132
+00:10:00,620 --> 00:10:04,020
+اشتعل فبدي اروح على طول احط عليه bucket of sand
+
+133
+00:10:04,020 --> 00:10:07,540
+يعني افتح الرقبة كده تحت الرمل و اكبها عليه علشان
+
+134
+00:10:07,540 --> 00:10:14,400
+يطفي fire blanket طبعا اللي هي البطانية المصنعة من
+
+135
+00:10:14,400 --> 00:10:19,650
+ال heavy cotton طبعاهي عبارة عن حاجة .. هي يعني
+
+136
+00:10:19,650 --> 00:10:22,610
+المادة اللي .. اللي مصنوعة منها هاي ال blankets
+
+137
+00:10:22,610 --> 00:10:26,910
+البطانيات fire retardant chemicals يعني من مواد
+
+138
+00:10:26,910 --> 00:10:34,370
+كيميائية يعني معيقة لنشوب الحرائق أو يعني إنها
+
+139
+00:10:34,370 --> 00:10:40,810
+طاردة للحرائق أو للنار فبالتالي بنستخدمها لما ..
+
+140
+00:10:43,410 --> 00:10:48,170
+بنطفي الحرائق سواء كانت يعني اشتعلت بالجسم أو لأ،
+
+141
+00:10:48,170 --> 00:10:54,650
+تمام؟ وش اسمه؟ ال dry powder chemical إذا طبعا في
+
+142
+00:10:54,650 --> 00:10:58,950
+بكيتات بيكون فيها dry powder، مواد powder كيميائية
+
+143
+00:10:58,950 --> 00:11:02,570
+بتستخدم علشان أطف فيها الحرائق اللي ناتجة من
+
+144
+00:11:02,570 --> 00:11:07,330
+اشتعال جهاز كهربائي أو حريق ناتج عن كهربا، تمام؟
+
+145
+00:11:07,330 --> 00:11:10,990
+إذا انتي عندى ال .. ال bucket of water علشان أطفي
+
+146
+00:11:11,130 --> 00:11:15,230
+ال .. الحرائق المشتعلة في ال paper و ال .. و ال
+
+147
+00:11:15,230 --> 00:11:21,170
+wood الخشب و ال sand للحرائق اللي مشتعلة في سوائل
+
+148
+00:11:21,170 --> 00:11:25,090
+و ال fire blanket لو كانت الحرائق لمثل جسمي أو
+
+149
+00:11:25,090 --> 00:11:31,590
+ملابسي و ال dry powder للحرائق الناتجة من التيار
+
+150
+00:11:31,590 --> 00:11:37,400
+الكهربائي، تمام؟طبعا عشان أنا أحمي نفسي من .. و
+
+151
+00:11:37,400 --> 00:11:42,480
+أنا بشتغل مع ال .. ال .. لها بنزن أو مع أي جهاز
+
+152
+00:11:42,480 --> 00:11:47,320
+بيعطيني حرارة أو بيعطي fire فبالتالي لازم أنا أربط
+
+153
+00:11:47,320 --> 00:11:51,320
+شعري للأعلى سواء كان للناس اللي مش محجبين يعني انا
+
+154
+00:11:51,320 --> 00:11:54,780
+حاكينها ان احنا محجبين و ماعنا هاي المشكلة طبعا و
+
+155
+00:11:54,780 --> 00:11:59,000
+انا بسخن في المحلول اذا كان .. لجأت لتسخين محلول
+
+156
+00:11:59,330 --> 00:12:03,070
+never look into container بطلعش في المحلول نفسه و
+
+157
+00:12:03,070 --> 00:12:06,290
+هو بيصخل لإن هو ممكن على طول يفور و ي .. و يلامس
+
+158
+00:12:06,290 --> 00:12:10,530
+وجهه never point the end of test tube being heated
+
+159
+00:12:10,530 --> 00:12:15,530
+to yourself or other يعني مثلا أنا بسخن في تيوب
+
+160
+00:12:15,530 --> 00:12:20,850
+على ال .. اللهب مش لازم فتحة التيوب تكون موجهة في
+
+161
+00:12:20,850 --> 00:12:24,910
+اتجاهي او باتجاه زميلتي لأ باتجاه الحائط لازم تكون
+
+162
+00:12:24,910 --> 00:12:29,470
+موجودة تمام؟never heat in a closed container
+
+163
+00:12:29,470 --> 00:12:32,630
+مابسخنش اللي أنا بيبي و هي مغلقة لإنها ممكن تنفجر
+
+164
+00:12:32,630 --> 00:12:33,250
+و تنكسر
+
+165
+00:12:37,010 --> 00:12:41,290
+طيب never leave a heat source unattended يعني
+
+166
+00:12:41,290 --> 00:12:45,330
+ماتسيبيش مصدر الحرارة أو مصدر اللهب متروك بدون
+
+167
+00:12:45,330 --> 00:12:48,290
+انتباه يعني احنا حكينا المحاضرة الماضية ممنوع اترك
+
+168
+00:12:48,290 --> 00:12:52,950
+لهب بنزين بدون يضل شغال و اروح اشوف شغل تاني او
+
+169
+00:12:52,950 --> 00:12:56,090
+اشتغل في مكان تاني لأ بخلص بسكر لهب بنزين و بأمن
+
+170
+00:12:56,090 --> 00:13:00,150
+الغاز بعدين بنتقل الى مكان اخرheated metal and
+
+171
+00:13:00,150 --> 00:13:05,730
+glass looks cool use forceps or gloves before
+
+172
+00:13:05,730 --> 00:13:11,070
+handling اذا انا بدى استخدم ال heat glass او metal
+
+173
+00:13:11,070 --> 00:13:14,230
+يعني بستخدم مادة معدنية او زجاجية ساخنة اما
+
+174
+00:13:14,230 --> 00:13:17,310
+باستخدام ال gloves او باستخدام ال forceps و هذا
+
+175
+00:13:17,310 --> 00:13:20,370
+كلام احنا حاكيناه don't place hot glassware
+
+176
+00:13:20,370 --> 00:13:23,670
+directly into the lab desk or in cold water
+
+177
+00:13:23,670 --> 00:13:28,960
+الزجاجيات الساخنة هذهمابحطهاش مباشرة على ال bench
+
+178
+00:13:28,960 --> 00:13:32,640
+علشان ما تحرق ال bench و بنفس الوقت ما بحطها على
+
+179
+00:13:32,640 --> 00:13:37,720
+ماء وط .. على ماء بارد يعني او ما بخلي الماء
+
+180
+00:13:37,720 --> 00:13:40,920
+البارد يلامسها لإنها ممكن تنكسر يعني نفس فكرة ال
+
+181
+00:13:40,920 --> 00:13:45,060
+birex إذا كان ساخن و لمس ماء باردnever heat the
+
+182
+00:13:45,060 --> 00:13:48,480
+bottom of the test tube طبعا انا مابركزش النار و
+
+183
+00:13:48,480 --> 00:13:52,360
+انا ب .. و انا حاطة التيوب على اللهب مابركزش النار
+
+184
+00:13:52,360 --> 00:13:55,960
+تكون فقط موجودة على قاع التيوب لأ بالعكس انا بمرر
+
+185
+00:13:55,960 --> 00:13:59,960
+التيوب على اللهب على أساس انه يكون في تسخين منتضم
+
+186
+00:13:59,960 --> 00:14:03,680
+لكل أجزاء التيوب مع بعضها البعضلأنه ممكن يسيطن
+
+187
+00:14:03,680 --> 00:14:09,380
+اتفور المادة او تنفجر التيوب او تنكسر فهذا خطأ
+
+188
+00:14:09,380 --> 00:14:12,980
+يعني خطأ لازم اوزع الحرارة يعني انا و انا ماسكة
+
+189
+00:14:12,980 --> 00:14:17,980
+التيوب زي هيك امررها على اللهب حتى تتوزع درجة
+
+190
+00:14:17,980 --> 00:14:23,360
+الحرارة بامتزام على مكونات التيوب طبعا ال sharp
+
+191
+00:14:23,360 --> 00:14:29,190
+objectsطبعا احنا هنستخدم شفرات هنستخدم needles ابر
+
+192
+00:14:29,190 --> 00:14:34,730
+مقصات في المختبر فبالتالي اول حاجة اذا انا بدي اقص
+
+193
+00:14:34,730 --> 00:14:38,710
+اي شي مش لازم انه يعني لازم يكون بعيد عن قصابي و
+
+194
+00:14:38,710 --> 00:14:43,370
+بعيد عن جسمي بحيث انه انا ما اجرح حالي دايما
+
+195
+00:14:43,370 --> 00:14:47,950
+الأدوات الحاد ده بنحملها carry sharp object with
+
+196
+00:14:47,950 --> 00:14:52,520
+point and tip facing down and awayنايما أنا بمسك
+
+197
+00:14:52,520 --> 00:14:55,740
+المقص بحيث أنه الجزء الحاد تبعه أو الشفرة أو
+
+198
+00:14:55,740 --> 00:14:59,620
+السكينة .. الجزء الحاد تبعه مشار إلى الأسفل أو
+
+199
+00:14:59,620 --> 00:15:03,500
+بعيد يعني مابروحش مثلا زميلتي اتناولني تحكيلي
+
+200
+00:15:03,500 --> 00:15:07,380
+هاتيلي المقص أروح الجزء الحاد أوجهه باتجاهها في
+
+201
+00:15:07,380 --> 00:15:10,620
+وجهها لإنها ممكن تكون هي مش من تبها و يأذيها
+
+202
+00:15:11,060 --> 00:15:16,040
+فبالتالي لازم يكون مشار إلى الأسفل. Never try to
+
+203
+00:15:16,040 --> 00:15:20,040
+touch falling sharp instrument. إذا انكسر أدوات
+
+204
+00:15:20,040 --> 00:15:28,700
+حادة زجاجية مثلا وجعة شفرة أو أدوات حادة، وجع بيكر
+
+205
+00:15:28,700 --> 00:15:33,660
+وانكسر، إشي زجاجي انكسر، مش لازم أنا أروح أنمسه
+
+206
+00:15:33,660 --> 00:15:38,580
+بإيدي نهائيا علشان ما أجرح نفسي.hold sharp
+
+207
+00:15:38,580 --> 00:15:42,260
+instrument only on the holder طبعا دايما الأدوات
+
+208
+00:15:42,260 --> 00:15:47,540
+الحادة هاي بيكون إلها يعني container خاص فيها و
+
+209
+00:15:47,540 --> 00:15:51,480
+باتخلص فيها بال container الخاص فيها و طبعا إذا
+
+210
+00:15:51,480 --> 00:15:57,340
+انت حصل عن الإلك جرح أو اتعرضتي لنخزة من قبرة أو
+
+211
+00:15:57,340 --> 00:16:02,400
+من شفرةلازم تحكي لل .. لل teacher يعني للمدرس و
+
+212
+00:16:02,400 --> 00:16:06,240
+بالتالي ت .. هو بي .. بيقوم معاكي بإجراءات
+
+213
+00:16:06,240 --> 00:16:10,360
+الإسعافات الأولية اللي بتتضمن أول حاجة انعصر الجرح
+
+214
+00:16:10,360 --> 00:16:15,360
+منيح عشان يطلع شوية دم و بعد هيك أغسل المكان
+
+215
+00:16:15,360 --> 00:16:19,380
+بالماء و الصابون و أعقم بالكحول و أحط bandage أو
+
+216
+00:16:19,380 --> 00:16:25,950
+لفاف أو plaster إذا لزم الأمرطبعا إحنا حكينا إنه
+
+217
+00:16:25,950 --> 00:16:30,770
+الأدوات الحادة ما بتلفها في سلة المهملات إنما إلها
+
+218
+00:16:30,770 --> 00:16:35,310
+box خاص فيها اسمه sharp box صندوق الأدوات الحادة
+
+219
+00:16:35,310 --> 00:16:41,110
+طبعا إذا كان تم انسكاب أي مادة كيميائية أول حاجة
+
+220
+00:16:41,110 --> 00:16:43,730
+بديش .. ما .. مابنفع أنا أتركها أروح أكمل شغل و
+
+221
+00:16:43,730 --> 00:16:47,070
+بعدين أرجعلها لإن ممكن يكون كتير في مشاكلعلى طول
+
+222
+00:16:47,070 --> 00:16:49,990
+بدي أعملها clear up on time يعني بدي أنظفها في
+
+223
+00:16:49,990 --> 00:16:54,630
+وقتها dispose any hazardous material and as toxic
+
+224
+00:16:54,630 --> 00:16:58,870
+waste و المادة اللي .. اللي انكبت أو انكسرت بتخلص
+
+225
+00:16:58,870 --> 00:17:02,690
+منها كأنها على أساس أنها هي toxic طبعا ال messy
+
+226
+00:17:02,690 --> 00:17:05,840
+workers اللي .. اللي ..الناس اللي بتشتغل بفوضى
+
+227
+00:17:05,840 --> 00:17:11,100
+دايما هم عبارة عن poor worker يعني شغلهم ما ..
+
+228
+00:17:11,100 --> 00:17:15,060
+مافيش إنتاجية، مافيش نتائج دقيقة، مافيش إشي صحيح
+
+229
+00:17:15,060 --> 00:17:17,900
+فبالتالي هذه مشكلتهم
+
+230
+00:17:20,330 --> 00:17:25,770
+طبعا خطر الوجود استوانات الغاز أو السوائل المضغوطة
+
+231
+00:17:25,770 --> 00:17:29,310
+داخل .. أو الغازات المضغوطة داخل المختبر أول حاجة
+
+232
+00:17:29,310 --> 00:17:32,510
+طبعا ممنوع أنا أستخدم هذه الاستوانات بدون ما أكون
+
+233
+00:17:32,510 --> 00:17:36,470
+متدرب عليها و لازم أقلل عددها minimize the number
+
+234
+00:17:36,470 --> 00:17:40,350
+in the laboratory أقلل عددها داخل المختبر ال
+
+235
+00:17:40,350 --> 00:17:45,990
+cylinder طبعا هي عبارة عن شئ ثقيل فبالتالي ما بنفع
+
+236
+00:17:45,990 --> 00:17:50,080
+أناإذا سقطت ممكن يعني على رجلي أو إشي ممكن تعمل
+
+237
+00:17:50,080 --> 00:17:53,960
+أداء كتير كبير فبالتالي لازم أنا لما أجي أحركها من
+
+238
+00:17:53,960 --> 00:17:57,380
+مكان إلى مكان ما أستخدم العضلات إنما أستخدم
+
+239
+00:17:57,380 --> 00:18:04,520
+literally يعني أروح أجيب ال .. يعني أداء ب ..
+
+240
+00:18:04,520 --> 00:18:10,200
+بيلها عجلات بتتحرك و ب .. بحط ال cylinder عليها و
+
+241
+00:18:10,200 --> 00:18:13,540
+بنقلها من مكان لآخر تبع .. باستخدام literally
+
+242
+00:18:13,540 --> 00:18:18,700
+تمام؟ensure that they are connected when in use
+
+243
+00:18:18,700 --> 00:18:22,100
+طبعا لازم انا اتأكد انها موصلة منيح اثناء
+
+244
+00:18:22,100 --> 00:18:25,740
+استخدامها ما .. يعني مايكونش في اشياء فالتة او
+
+245
+00:18:25,740 --> 00:18:31,960
+اشياء رخوة لأ لازم تكون محكومة الإغلاق و هي محكومة
+
+246
+00:18:31,960 --> 00:18:38,160
+الاتصال بالكوابل تبعتها او اللي هي بالانابيب
+
+247
+00:18:38,160 --> 00:18:42,640
+تبعتها use regulator طبعا لازم يكون في عندى منظم
+
+248
+00:18:42,640 --> 00:18:46,960
+ليلها عشان يتحكملي بكمية الغاز اللي بتطلعليو أيضا
+
+249
+00:18:46,960 --> 00:18:51,140
+إذا كان في عندي أي مشكلة أو أي تسريب لازم أنا
+
+250
+00:18:51,140 --> 00:18:55,120
+أعالج المشكلة ب .. ب .. باستمرار لإن حكينا تسريب
+
+251
+00:18:55,120 --> 00:18:56,600
+الغاز ممكن يعمل حرائق.
+
+252
+00:18:59,800 --> 00:19:04,860
+Cryogenics في عندي غازات طبعا هي liquid أنا بضغطها
+
+253
+00:19:04,860 --> 00:19:10,840
+لحتى أحافظ لحتى أخزن مثلا أشياء على درجات حرارة
+
+254
+00:19:10,840 --> 00:19:16,880
+كتير كتير قليلة يعني مثلا الخلايا جسم الإنسان لما
+
+255
+00:19:16,880 --> 00:19:20,120
+أنا بدي أخزنها بخزنها على سالب سبعين و سالب تمانين
+
+256
+00:19:20,120 --> 00:19:25,950
+طبعا ما بصلش لدرجات الحرارة هذه إلا باستخدامغازات
+
+257
+00:19:25,950 --> 00:19:30,310
+طبعا ال liquid gases طبعا liquid gases اللي هي
+
+258
+00:19:30,310 --> 00:19:35,130
+عبارة عن هي extremely cold بتكون باردة كتير و ممكن
+
+259
+00:19:35,130 --> 00:19:38,610
+تسبب حرائق في حرائق بسبب البرودة زي ما في حرائق
+
+260
+00:19:38,610 --> 00:19:41,390
+بسبب ارتفاع درجات الحرارة او اللي هي عند ال heat
+
+261
+00:19:41,390 --> 00:19:46,050
+السخونة liquid gas evaporate ممكن انها هي هذه ال
+
+262
+00:19:46,050 --> 00:19:49,810
+liquid gases تتبخر و تعمللي acidification اختناق
+
+263
+00:19:49,810 --> 00:19:53,010
+فبالتالي لازم انا احافظ عليها ان هي ما تتبخر و ما
+
+264
+00:19:53,010 --> 00:19:58,070
+تتسربو تكون محكمة الإغلاق داخل الأنابيب تبعتها و
+
+265
+00:19:58,070 --> 00:20:01,630
+طبعا ما بستخدمها إلا ب training خاص فيها زي ال
+
+266
+00:20:01,630 --> 00:20:07,230
+liquid nitrogen أكيد سمعتوا عنهو بعدين ممكن انها
+
+267
+00:20:07,230 --> 00:20:11,130
+تكون قابلة للانفجار او الاحتراق ال electrical
+
+268
+00:20:11,130 --> 00:20:16,510
+equipment طبعا كيف انا اتعامل الحد من خطر الدرجات
+
+269
+00:20:16,510 --> 00:20:22,950
+او خطر الكهرباء اول حاجة بدي اعمل visual check على
+
+270
+00:20:22,950 --> 00:20:25,790
+ال electrical equipment حكينا المحاضرة الماضية انه
+
+271
+00:20:25,790 --> 00:20:28,570
+الأدوات الكهربائية لازم باستمرار انا اشيك عليها
+
+272
+00:20:28,820 --> 00:20:33,800
+أفقي نظرة عليها، أشوف درجة حرارتها، أشوف هل هي
+
+273
+00:20:33,800 --> 00:20:37,160
+مشبوك، هل هي بتشتغل بانتظام أو لأ، فيها خلال ما أو
+
+274
+00:20:37,160 --> 00:20:41,120
+فيها عطل، بتعطيني alarm يعني انذار انه والله انه
+
+275
+00:20:41,120 --> 00:20:44,740
+هي .. هو هذا الجهاز خربان، فهذا كله لازم أكون
+
+276
+00:20:44,740 --> 00:20:49,600
+متعاملة معاه، بعدين كمان all portable electrical
+
+277
+00:20:49,600 --> 00:20:53,340
+equipment must have a current path test يعني لازم
+
+278
+00:20:53,340 --> 00:20:58,930
+يكون فيه على كل ال .. الأجهزة الكهربائيةعبارة عن
+
+279
+00:20:58,930 --> 00:21:02,410
+sticker بسميه portable appliance testing يعني هذا
+
+280
+00:21:02,410 --> 00:21:05,730
+ال sticker بيحكيلي أن هو شغال ولا مش شغال، إذا كان
+
+281
+00:21:05,730 --> 00:21:11,370
+فيه مشكلة أو لأ، إمتى تم تشغيله، طبعا إيش محتاج
+
+282
+00:21:11,370 --> 00:21:14,130
+ملاحظات أثناء تشغيله، هذه كلها بتكون موجودة على ال
+
+283
+00:21:14,130 --> 00:21:17,830
+portable appliance testing sticker طبعا never use
+
+284
+00:21:17,830 --> 00:21:21,110
+defective equipment إذا الجهاز مكتوب لك عليه أن هو
+
+285
+00:21:21,110 --> 00:21:26,400
+هذا defective، فيه خللعطلان major بتستخدميه نهائيا
+
+286
+00:21:26,400 --> 00:21:31,560
+ما تحاولي فيه تمام و طبعا only electrical plugs
+
+287
+00:21:31,560 --> 00:21:34,840
+are to be placed into electrical outlet طبعا بس
+
+288
+00:21:34,840 --> 00:21:38,060
+الأداء الأسلاك الكهربائية هي اللي احنا الكبش هي
+
+289
+00:21:38,060 --> 00:21:44,570
+اللي بنحطها داخل ال ..يعني داخل الطيار الكهربائي
+
+290
+00:21:44,570 --> 00:21:51,290
+أما الأدوات الحادة لأ أقلام أشياء تانية لأ unplug
+
+291
+00:21:51,290 --> 00:21:54,570
+electrical equipment after use بعد ما أخلص استخدام
+
+292
+00:21:54,570 --> 00:21:59,450
+الجهاز لازم طبعا أرفع الأكوابل أفصلها ما أخليها
+
+293
+00:21:59,450 --> 00:22:03,110
+مشبوكة بالكهربا و طبعا كل ال electrical cord
+
+294
+00:22:03,110 --> 00:22:08,080
+الأحبال و الأسلاك الكهربائيةلازم تكون بعيدة عن
+
+295
+00:22:08,080 --> 00:22:12,260
+الماء و لازم طبعا الماء ألمسها إذا كانت هي مكشوفة
+
+296
+00:22:12,260 --> 00:22:20,560
+طيب ال chemical safety ال chemical safety أول حاجة
+
+297
+00:22:20,560 --> 00:22:23,240
+بحكيلك عن المواد الكيميائية طبعا هتكون عندنا
+
+298
+00:22:23,240 --> 00:22:28,500
+زجاجات كيميائية زي هذا الشكل بهك بهذا الشكل هك
+
+299
+00:22:30,830 --> 00:22:35,650
+طبعا زي ما أنتوا شايفين و هذه الزجاجات عليها
+
+300
+00:22:35,650 --> 00:22:40,430
+علامات طبعا العلامات هذه أو يعني ال label هذا
+
+301
+00:22:40,430 --> 00:22:46,350
+الأوراق الملثقة على الزجاجات
+
+302
+00:22:46,350 --> 00:22:50,810
+فيها معلومات بتخص كل .. كل إشي بيتعلق بالمادة
+
+303
+00:22:50,810 --> 00:22:54,770
+الكيميائية، استخدامها، درجة حرارة حفظها، تاريخ
+
+304
+00:22:54,770 --> 00:22:59,970
+فتحها، تاريخ انتهاءهاتركيبتها الكيميائية تعليمات
+
+305
+00:22:59,970 --> 00:23:06,230
+لا استخدامها تحذيرات استخدامها بشكل خطأكل إشي
+
+306
+00:23:06,230 --> 00:23:09,230
+بيكون موجودة على هذا ال label فبالتالي لازم انا
+
+307
+00:23:09,230 --> 00:23:12,650
+أقرأ ال label هذا twice بده أقرأه بدل المرة مرتين
+
+308
+00:23:12,650 --> 00:23:17,850
+علشان أتأكد أن أنا فاهمة كل إشي بيتعلق بهذه المادة
+
+309
+00:23:17,850 --> 00:23:21,790
+قبل استخدامها طبعا only use the type and amount of
+
+310
+00:23:21,790 --> 00:23:25,490
+chemical instructed طبعا أنا بستخدم نفس نوع المادة
+
+311
+00:23:25,490 --> 00:23:30,150
+و نفس الكمية في التفاعل الكيميائي مثلا حكالي والله
+
+312
+00:23:30,150 --> 00:23:33,950
+في التفاعل الكيميائي ضيفي 2 ml of etherدور
+
+313
+00:23:33,950 --> 00:23:38,650
+بالخزانة فتش فتش فتش مافيش ether لاجت acetone يلا
+
+314
+00:23:38,650 --> 00:23:41,610
+ماهو ether ولا acetone إخوان بعض خليني أضيف
+
+315
+00:23:41,610 --> 00:23:46,390
+acetone اتنين ML acetone والله ما أنا مش عاجبنك
+
+316
+00:23:46,390 --> 00:23:49,810
+أنه أحط اتنين ML مش هيك مش حاسة أنه النتيجة هتطلع
+
+317
+00:23:49,810 --> 00:23:54,080
+مظبوطةخليني أحط تلاتة أمال، هذا خطأ، لازم أستخدم
+
+318
+00:23:54,080 --> 00:23:57,340
+نفس المادة الكيميائية المشارقليها بالتجربة و نفس
+
+319
+00:23:57,340 --> 00:24:00,980
+الكمية، مابنفعش انا هيك يعني يكون في عندي حس الطبخ
+
+320
+00:24:00,980 --> 00:24:04,820
+و الحس الطباخ و أحس أنه انا اتنين أمال، لأ لأ مش
+
+321
+00:24:04,820 --> 00:24:11,510
+كافين، زودوا، لأ بنفعشتمام؟ ف .. و .. و كمان هذه
+
+322
+00:24:11,510 --> 00:24:14,710
+المعلومة مرت معانا never touch ممنوع ألمس ممنوع
+
+323
+00:24:14,710 --> 00:24:19,070
+أتضوخ ممنوع أشتم أي مادة كيميائية ممنوع أخلطهم مع
+
+324
+00:24:19,070 --> 00:24:22,410
+بعض المواد الكيميائية بدون ما ال .. ال mess تحكيلي
+
+325
+00:24:22,410 --> 00:24:26,590
+انا حكيتلك اعملي .. ضيفي ال .. المادة A على المادة
+
+326
+00:24:26,590 --> 00:24:30,990
+B و .. و .. و .. و اعمليلهم mix كويس بتضيفيهمبدون
+
+327
+00:24:30,990 --> 00:24:35,830
+ما انا احكي رجاء الشخص الكيميائي او ال .. ال .. ال
+
+328
+00:24:35,830 --> 00:24:39,090
+.. الانسان الكيميائي اللي جواتك اقتليه قبل لاتفوت
+
+329
+00:24:39,090 --> 00:24:44,500
+المختبر لإنه هيودينا في دهيا.transfer chemical
+
+330
+00:24:44,500 --> 00:24:47,920
+carefully طبعا بدي أنقل المواد الكيميائية من مكان
+
+331
+00:24:47,920 --> 00:24:51,540
+إلى أخر بحذر بحيث انه انا يعني ما أصطدم مثلا
+
+332
+00:24:51,540 --> 00:24:55,220
+بزميلتي و انا ماشية اكب عليها او ينكب عليا keep
+
+333
+00:24:55,220 --> 00:24:58,060
+lid on chemical container لازم تكون المواد
+
+334
+00:24:58,060 --> 00:25:02,500
+الكيميائية مغطاة تمام handal acid and alkalis
+
+335
+00:25:02,500 --> 00:25:07,480
+firmly upright and dry hand لازم انا بستخدم المواد
+
+336
+00:25:07,480 --> 00:25:12,700
+الحمضية و القاعدية بأيدي جافةو بأيدي لابسة gloves
+
+337
+00:25:12,700 --> 00:25:18,620
+و باستخدام التعليمات اللي حتى أحافظ على نفسي never
+
+338
+00:25:18,620 --> 00:25:22,300
+pour water into strong acid because the danger of
+
+339
+00:25:22,300 --> 00:25:25,320
+reaction and splashing يعني مابعرفش عندي strong
+
+340
+00:25:25,320 --> 00:25:29,940
+acid HCl ولا H2SO4 و أنا بدي أخففه و أضيف عليه
+
+341
+00:25:29,940 --> 00:25:35,240
+water مباشرة لأ مابنفعشيعني لإنه هذا ممكن يقدي إلى
+
+342
+00:25:35,240 --> 00:25:39,100
+إيش انه يصير splashing تطير لأ ال acid و يبتدي
+
+343
+00:25:39,100 --> 00:25:42,280
+يصير حارق طبعا انا كده طبعا إيش بدي أعمل لما بدي
+
+344
+00:25:42,280 --> 00:25:46,480
+أخفف ال acid بروح بضيف ال acid للماء و ليس الماء
+
+345
+00:25:46,480 --> 00:25:50,530
+لل acid تتذكر المحاضرة الماضية إيش حكيتلكم؟حكيتلكم
+
+346
+00:25:50,530 --> 00:25:54,010
+حتى ترتيب الخطوات كويس عشان هيك لما .. لما هنشتغل
+
+347
+00:25:54,010 --> 00:25:57,630
+مع بعض بالمختبر هنكون ماشيين خطوة خطوة كلكوا يلا
+
+348
+00:25:57,630 --> 00:26:01,010
+اضيفوا المادة الكولانية الكل ده يلا ننتقل للخطوات
+
+349
+00:26:01,010 --> 00:26:05,630
+التانية وها كذا لإنه فعليا انا لما اجي احكي للطالب
+
+350
+00:26:05,630 --> 00:26:10,510
+اضيفي اخفف ال acid ب water فعليا ان انت تضيفي ال
+
+351
+00:26:10,510 --> 00:26:14,670
+water لل acid اشي مصيبةلكن انك انت مثلا ضيف ال
+
+352
+00:26:14,670 --> 00:26:19,110
+acid لالماء بالتدريج و ع الجدار و شوي شوي هيك
+
+353
+00:26:19,110 --> 00:26:23,310
+بيكون بطريقة صحيحة احنا بنخفف ال acid و ما .. بنسي
+
+354
+00:26:23,310 --> 00:26:27,570
+.. بنعرض نفسنا لخطر انه يتطير المادة الحامضية قوية
+
+355
+00:26:27,570 --> 00:26:31,350
+هاي الحارقة و انه نؤذي نفسنا او نؤذي زملائنا او
+
+356
+00:26:31,350 --> 00:26:32,430
+المكان المحيط فينا
+
+357
+00:26:35,310 --> 00:26:38,770
+طيب don't keep acid and alkalis in bottle with the
+
+358
+00:26:38,770 --> 00:26:42,990
+ground glasses to bare طيب ال .. ال .. مواقع
+
+359
+00:26:42,990 --> 00:26:47,230
+الأحماض و القواعد هذه بنحطها احنا في أنابيب زجاجية
+
+360
+00:26:47,230 --> 00:26:49,990
+بس لازم الأنابيب الزجاجية تكون غطيتها plastic
+
+361
+00:26:49,990 --> 00:26:54,430
+ماتكونش غطيتها glass لإن ممكن ال glass هذه تصير ..
+
+362
+00:26:54,430 --> 00:26:59,050
+يعني تصير ملتصقة و صعب تفتح they .. they may get
+
+363
+00:26:59,050 --> 00:27:03,590
+stuck ممكن تلتصق يعني تصير عالقة مش راضية تفتح
+
+364
+00:27:03,590 --> 00:27:07,500
+النبوبةconsider all chemical dangerous طبعا انت
+
+365
+00:27:07,500 --> 00:27:10,060
+بديك تتعاملي مع كل المواد الكيميائية انها خاطرة
+
+366
+00:27:10,060 --> 00:27:14,340
+علشان تكون بتحمي نفسك كلها تتعاملي معاها بحضر و
+
+367
+00:27:14,340 --> 00:27:18,380
+طبعا بنستخدم ال by bit that are plugged with non
+
+368
+00:27:18,380 --> 00:27:22,260
+absorbent cotton wool with rubbertube attachment
+
+369
+00:27:22,260 --> 00:27:26,260
+بنستخدم الأنواع مخصصة من ال by بتعشان ننقل الحجوم
+
+370
+00:27:26,260 --> 00:27:30,260
+فيها يعني ال acid و ال base هناخدها بالمختبر و
+
+371
+00:27:30,260 --> 00:27:33,460
+أيضا every container of chemical is labeled with
+
+372
+00:27:33,460 --> 00:27:36,820
+by manufacturer حكينا إنه كل مادة كيميائية
+
+373
+00:27:36,820 --> 00:27:40,600
+الهوايقة تبعها بيكون عليه label جاي من الشركة
+
+374
+00:27:40,600 --> 00:27:45,160
+المصنعة كل إشي محطوط عليه مادة الكيميائية اسمها
+
+375
+00:27:45,160 --> 00:27:51,010
+name of chemical nameاسم و عنوان و ال emergency
+
+376
+00:27:51,010 --> 00:27:55,210
+phone للمادة المصنعة .. للشركة المصنعة يعني ال ..
+
+377
+00:27:55,210 --> 00:27:58,790
+ال .. الاسم و العنوان و التليفون، طوارئ تبع الشركة
+
+378
+00:27:58,790 --> 00:28:04,290
+المصنعة في حال لو حدث مشكلةphysical and health
+
+379
+00:28:04,290 --> 00:28:08,090
+hazard يعني هذه المادة ده ايش خطرها عليكي ممكن
+
+380
+00:28:08,090 --> 00:28:11,730
+تكون موجودة بيعيقيكي تعليمات ان هي ممكن تكون حارقة
+
+381
+00:28:11,730 --> 00:28:14,970
+ممكن تكون بتسبب تلف للانتجار ممكن تكون سامة هذا
+
+382
+00:28:14,970 --> 00:28:17,130
+كله بيكون موجود على ال bottle من برة عشان هيكي
+
+383
+00:28:17,130 --> 00:28:19,850
+احنا لازم نقرأ هاي ال .. ال label اللي موجودة على
+
+384
+00:28:19,850 --> 00:28:22,900
+ال bottlefirst-hand instruction بيعطيكي تعليمات
+
+385
+00:28:22,900 --> 00:28:25,560
+ايضا اسعاف اول لو هاي المادة انسكبت عليكي ايش
+
+386
+00:28:25,560 --> 00:28:29,200
+تعملي او انت بالغلط استنشقتيها او بالغلط ايش لمست
+
+387
+00:28:29,200 --> 00:28:32,700
+ايداكي و ايضا proper handling and storage
+
+388
+00:28:32,700 --> 00:28:35,960
+instruction و كيف انت تتعاملي معاها تستخدميها و
+
+389
+00:28:35,960 --> 00:28:39,520
+كيف تخزنيها ايضا هذه التعليمات كلها بتكون موجودة
+
+390
+00:28:39,520 --> 00:28:40,680
+على ال label
+
+391
+00:28:44,270 --> 00:28:48,650
+physical safety طيب handle all equipment carefully
+
+392
+00:28:48,650 --> 00:28:53,110
+بدا اتعامل مع كل الأدوات عندي و الأجهزة بحذر و
+
+393
+00:28:53,110 --> 00:28:56,990
+طبعا الأسلاك اللي بتكون موجودة داخل المختبر انا مش
+
+394
+00:28:56,990 --> 00:29:01,240
+لازم انا امددها و تكون بتقطع طريقة عشانما اتعرقل
+
+395
+00:29:01,240 --> 00:29:04,980
+فيها و اجعل خصوصا و انا مسك ادوات زجاجية او محالي
+
+396
+00:29:04,980 --> 00:29:09,580
+بنجلهم من بنش ل بنش push all stools in and out the
+
+397
+00:29:09,580 --> 00:29:13,340
+way طبعا لازم تكون الكراسي مجرد ما انت تجومي عن
+
+398
+00:29:13,340 --> 00:29:17,890
+الكرسي اتفوتي في مكانه بحيث انه مايكنمرمى فى نص
+
+399
+00:29:17,890 --> 00:29:21,710
+اللى بتيجى بزميلتك تنخبط فيه او ماشية مش من تبهاله
+
+400
+00:29:21,710 --> 00:29:27,310
+انها يعني تسقط او يجع منها اشي او ينكسر اشي او
+
+401
+00:29:27,310 --> 00:29:32,330
+ينكب عليها اشي تمام؟ و ايضا لازم يكون المكان بشكل
+
+402
+00:29:32,330 --> 00:29:38,080
+عاماللي بنشتغل فيه tidy ال work place تبعي المكان
+
+403
+00:29:38,080 --> 00:29:42,920
+اللي عامل لازم يكون tidy نظيف لازم أتخلص من ال
+
+404
+00:29:42,920 --> 00:29:47,120
+waste أول بأول clear up waste و طبعا حكينا احنا أن
+
+405
+00:29:47,120 --> 00:29:50,380
+ال waste تبع الفضلات كل نوع أتخلص فيه في المكان
+
+406
+00:29:50,380 --> 00:29:56,400
+المناسب لإله و أيضا لإن ال tidy laboratory المختبر
+
+407
+00:29:56,400 --> 00:30:01,640
+النظيف avoid accidents بيمنعني من حدوث أو بيجنبني
+
+408
+00:30:01,640 --> 00:30:07,900
+من حدوثال .. الحوادث او المخاطر تمام ال laboratory
+
+409
+00:30:07,900 --> 00:30:11,680
+equipment never use any laboratory equipment
+
+410
+00:30:11,680 --> 00:30:16,240
+unless you are trained طبعا اي جهاز او اي ادا
+
+411
+00:30:16,240 --> 00:30:19,460
+ممنوعة تستخدميها الا لما تكوني اتدربتي عليها او
+
+412
+00:30:19,460 --> 00:30:23,300
+انا اعطيتك اشارة انك تستخدميها او انه علمتك
+
+413
+00:30:23,300 --> 00:30:29,580
+شريحتليك كيفية استخدامهاو بالتالي كمان لإنه ..
+
+414
+00:30:29,580 --> 00:30:32,720
+يعني ان انت تئدي نفسك او انك تخربي الجهاز او تخربي
+
+415
+00:30:32,720 --> 00:30:37,120
+ال .. الأداء او انك انت مثلا اي حد يطلق ضرر معين
+
+416
+00:30:37,120 --> 00:30:41,920
+نتيجة استخدامك الخاطئ لها، هاد طبعا تمانه او عراقه
+
+417
+00:30:41,920 --> 00:30:46,840
+وخيمة.ال hazard symbols ايش يعني ال hazard
+
+418
+00:30:46,840 --> 00:30:52,420
+symbols؟ هي عبارة عن symbols designed to warn
+
+419
+00:30:52,420 --> 00:30:57,540
+about hazardous material تمام؟ و location and or
+
+420
+00:30:57,540 --> 00:31:02,600
+objects يعني هي عبارة عنrecognizable symbols رموز
+
+421
+00:31:02,600 --> 00:31:08,920
+يمكن إدراكها ومصممة design to مصممة لتحذرني to
+
+422
+00:31:08,920 --> 00:31:12,760
+warn على إيش تحذرني على ال hazardous material
+
+423
+00:31:12,760 --> 00:31:19,520
+المواد الخطرة أماكنها و الاشياء اللي موجودة فيها و
+
+424
+00:31:19,520 --> 00:31:24,840
+أيضا لتحذرني انه على خطر ال electric current
+
+425
+00:31:24,840 --> 00:31:29,060
+التيار الكهربائي والسموم والإشعاعاتهذه كلها بتكون
+
+426
+00:31:29,060 --> 00:31:32,560
+موجودة طبعا في المختبر و حكينا أنها بتكون موجودة
+
+427
+00:31:32,560 --> 00:31:35,420
+على الحائط على ال cabinets على الأدوات الزجاجية
+
+428
+00:31:35,420 --> 00:31:41,040
+على المواد الكيميائية لحتى اتحذرني من خطر و شرحنا
+
+429
+00:31:41,040 --> 00:31:45,940
+هذه ال symbols أو هذه الرموز في المحاضرة الماضية
+
+430
+00:31:47,950 --> 00:31:54,770
+تمام ال first aid كيف هي عملية الإسعاف الأولى إذا
+
+431
+00:31:54,770 --> 00:32:00,450
+حدث hazard إذا حدث خطر معين داخل المختبر طبعا أول
+
+432
+00:32:00,450 --> 00:32:07,910
+حاجة أنه لازم أكون يعني بحكيلك أنه ماعرفت ماذا يجب
+
+433
+00:32:07,910 --> 00:32:13,090
+عليك أنت تتصرف يقيك العواقب تمام knowing what to
+
+434
+00:32:13,090 --> 00:32:17,210
+do immediately ماعرفت إيش لازم تعملي في الحالif an
+
+435
+00:32:17,210 --> 00:32:21,770
+accident occur إذا حدث حادث أو حصل حادث تمام can
+
+436
+00:32:21,770 --> 00:32:25,690
+help you بتساعدك to reduce suffering تقلل معاناتك
+
+437
+00:32:25,690 --> 00:32:28,090
+و تقلل من ال serious damage أو ال serious
+
+438
+00:32:28,090 --> 00:32:31,530
+accidents أو العواقب ال consequences اللي ممكن
+
+439
+00:32:31,530 --> 00:32:38,010
+تحدثلك من هذه ال accidents تمام فبالتالي ب .. في
+
+440
+00:32:38,010 --> 00:32:42,070
+بعض الحالات ال first aid الإسعاف الأولى can be
+
+441
+00:32:42,070 --> 00:32:46,850
+life saving يعني ممكن يع .. يعنيينقذ حياة الشخص زي
+
+442
+00:32:46,850 --> 00:32:50,210
+مثلا إن أنا أعمل control of bleeding شخص صار عنده
+
+443
+00:32:50,210 --> 00:32:55,710
+cut injury جرح نتيجة إخدامه أدى حد بشكل خطأ لو أنا
+
+444
+00:32:55,710 --> 00:32:59,410
+عملت control لل bleeding هذا معناه .. معناه انا ..
+
+445
+00:32:59,410 --> 00:33:04,770
+انا حافظت على حياة الشخص هذا تمام فبالتالي لازم ال
+
+446
+00:33:04,770 --> 00:33:10,490
+laboratory worker لازم يكون عندهمتعليمات حوالين
+
+447
+00:33:10,490 --> 00:33:14,270
+الإسعافات الأولية و أعتقد أنتوا عندكم مادة كاملة
+
+448
+00:33:14,270 --> 00:33:18,310
+عن الإسعافات الأولية بتعلمكم في إيش ممكن .. كيف
+
+449
+00:33:18,310 --> 00:33:23,570
+ممكن تتصرفوا في أكتر من موقف لحتى يكون في عندكم
+
+450
+00:33:23,570 --> 00:33:28,660
+خلفية how to first aidmyself and my colleagues
+
+451
+00:33:28,660 --> 00:33:35,640
+طبعا لازم نعرف احنا اش منا نستخدم اش منا نعمل او
+
+452
+00:33:35,640 --> 00:33:38,780
+اش منا نتصرف في ال emergency action في حالات
+
+453
+00:33:38,780 --> 00:33:43,300
+التوارق برضه كمان لو مثلا عندي مريض أغم عليه
+
+454
+00:33:43,300 --> 00:33:49,700
+collapse مريض اختنق مريض قدردت فعل عكسية اثناء
+
+455
+00:33:49,700 --> 00:33:54,340
+مثلا سحب عينة الدم بخاف هو عادي بتصيرفهذه كلها
+
+456
+00:33:54,340 --> 00:33:58,500
+برضه كمان لازم أكون بعرف أتصرف فيها المواقف هاي
+
+457
+00:33:58,500 --> 00:34:04,100
+طبعا لازم يكون في عندى adequately equipped first
+
+458
+00:34:04,100 --> 00:34:08,180
+aid box تمام لازم يكون عندى صندوق للإسعافات
+
+459
+00:34:08,180 --> 00:34:11,900
+الأولية adequately equipped adequate يعني كافي
+
+460
+00:34:11,900 --> 00:34:15,300
+equipped محوج يعني محوج بشكل كافي يعني موجود فيه
+
+461
+00:34:15,300 --> 00:34:18,830
+كل الأدواتبشكل كافي فيه ال plasters و ال .. و
+
+462
+00:34:18,830 --> 00:34:23,870
+الشاشة و الكحول و ال iodine و ال .. و فيه كل إشي
+
+463
+00:34:23,870 --> 00:34:29,330
+اللي بيتعلق بالإسعافات الأولية ماسكات بلاسترات كل
+
+464
+00:34:29,330 --> 00:34:34,610
+إشي تمام؟و لازم هذا ال box باستمرار باستمرار أنا
+
+465
+00:34:34,610 --> 00:34:39,810
+أعمله examine عشان أشيك عليه إيش نقص منه أ .. أ ..
+
+466
+00:34:39,810 --> 00:34:44,590
+أعبيه مرتان و أخليه جاهز و يخليه متوفر فيه كل
+
+467
+00:34:44,590 --> 00:34:48,810
+الأدوات اللي بتكون موجودة عندىطبعا لازم يكون هذا
+
+468
+00:34:48,810 --> 00:34:52,010
+ال box مصنوع من ال metal أو ال plastic علشان ما
+
+469
+00:34:52,010 --> 00:35:01,470
+يتكسر و برضه كمان عشان .. و لازم يكون نظيف و مغلق
+
+470
+00:35:01,470 --> 00:35:06,610
+و موجود في مكان الكل بيعرف يصله لحتى نسهل من عملية
+
+471
+00:35:06,720 --> 00:35:10,280
+ال first aiding اللي هي الإسعافات الأولية طبعا إيش
+
+472
+00:35:10,280 --> 00:35:15,480
+مكونات ال .. الإسعافات .. صندوق الإسعافات الأولية
+
+473
+00:35:15,480 --> 00:35:19,780
+هى بيكون لازم يكون فيه sterile dressing يعني شاش
+
+474
+00:35:19,780 --> 00:35:23,970
+معقم عشان نغطي الجروح to cover woundabsorbent
+
+475
+00:35:23,970 --> 00:35:27,750
+cotton .. cotton عشان يمتص السوائل أو المواد
+
+476
+00:35:27,750 --> 00:35:31,430
+المعاقمة أو أي إشي لازم يكون في عن .. عندي roll
+
+477
+00:35:31,430 --> 00:35:35,550
+bandage اللي هي اللزج اللي عامل زي رولات أو حتى
+
+478
+00:35:35,550 --> 00:35:38,890
+اللي ماخد شكل المتلتات زي اللي بنحط على العين أو
+
+479
+00:35:38,890 --> 00:35:43,310
+إشياسترايل ايد باد لازم برضه كمان قطن خاص بالعين
+
+480
+00:35:43,310 --> 00:35:51,670
+معقّم رول أدهيس الشاش لاصق او شريط لاصق سيزورز
+
+481
+00:35:51,670 --> 00:35:54,890
+مقصات لازم يكون موجود فيه Sodium Bicarbonate
+
+482
+00:35:54,890 --> 00:35:58,470
+Powder و Boric Acid Powder و 5% Acetic Acid و
+
+483
+00:35:58,470 --> 00:36:01,810
+Magnesium Hydroxide Suspension طبعا هذه الأربع
+
+484
+00:36:01,810 --> 00:36:05,590
+محليل او المواد الكيميائية راح نعرف استخدامها في
+
+485
+00:36:05,590 --> 00:36:06,830
+ال slides الجاية
+
+486
+00:36:09,620 --> 00:36:15,030
+تمامإيش بحدث لو صار في عندي cut جرح و bleeding إيش
+
+487
+00:36:15,030 --> 00:36:18,330
+بيصير؟ أول إشي بدي أغسل المكان بالماء و الصابون،
+
+488
+00:36:18,330 --> 00:36:21,670
+تمام؟ بعدين حكينا apply pressure with a piece of
+
+489
+00:36:21,670 --> 00:36:26,970
+cotton، بدي أسكر الجرح بقطعة قطن، تمام؟ أعقم
+
+490
+00:36:26,970 --> 00:36:31,910
+المكان و ألفه، أعقمه ب iodine و ألفه، تمام؟ cover
+
+491
+00:36:31,910 --> 00:36:36,070
+.. هي هنا disinfect، بعقم و cover، بلفه، تمام؟
+
+492
+00:36:36,070 --> 00:36:43,710
+بالشاشة إذا كان الجرحطبعا، يعني أنا انجرحت بمادة
+
+493
+00:36:43,710 --> 00:36:48,390
+ملوثة، إيش أعمل؟ أولا بدي أخسل إيدي بالماء
+
+494
+00:36:48,390 --> 00:36:52,090
+والصابون، بعدين بدي أعمل encouraging bleeding of
+
+495
+00:36:52,090 --> 00:36:55,450
+two minutes، يعني أنا بدي أعصر إيدي و أخليها تنزف
+
+496
+00:36:55,450 --> 00:36:59,770
+لمدة two minutes، طبعا، ليش؟ لإن الدم هذا اللي
+
+497
+00:36:59,770 --> 00:37:03,680
+بيطلع من الجروح بياخد ال contaminationالمواد
+
+498
+00:37:03,680 --> 00:37:07,540
+الملوثة اللي .. اللي دخلت جوا الجرح و بطلعها لبره،
+
+499
+00:37:07,540 --> 00:37:11,200
+زي كأني أنا قاعدة بعمل washing بال bleeding تبعي،
+
+500
+00:37:11,200 --> 00:37:16,600
+تمام؟ بخسل تاني بالماء و الصابون و برجع بطبق نفس
+
+501
+00:37:16,600 --> 00:37:20,440
+الأدوات و إذا كان الجارح عميق ممكن أنا أسأل .. او
+
+502
+00:37:20,440 --> 00:37:26,080
+يعني الدكتور يجي يعمل قطب غرز طبية او .. او ما
+
+503
+00:37:26,080 --> 00:37:26,500
+شابه
+
+504
+00:37:29,320 --> 00:37:35,180
+طبعا، لو كان في عندي حروق باستخدام الحرارة، لو حدث
+
+505
+00:37:35,180 --> 00:37:37,960
+حرق باستخدام الحرارة، أنا لمست اشي ساخن بدون ما
+
+506
+00:37:37,960 --> 00:37:42,040
+أنتبه، إيش بدي أعمل؟ على طول immerse the burnt
+
+507
+00:37:42,040 --> 00:37:46,180
+area، المكان اللي صار له احتراقعلى طول بغمروا تحت
+
+508
+00:37:46,180 --> 00:37:52,980
+الماء البارد و طبعا or apply soaked bed with cold
+
+509
+00:37:52,980 --> 00:37:57,840
+water يعني يا إما بغمر إيدي تحت ماء بارد جاري لمدة
+
+510
+00:37:57,840 --> 00:38:02,680
+عشر دقائق أه بحط قطنة فيها ماء بارد على المكان زي
+
+511
+00:38:02,680 --> 00:38:07,200
+كمادة يعني لمدة عشر دقائق و cover with dry
+
+512
+00:38:07,200 --> 00:38:11,200
+dressing و بعدين بغطي المكان بشاشة إذا كان الجرح
+
+513
+00:38:11,200 --> 00:38:16,420
+severe أو الحرق severeيعني اطول اكيد بوجه الشخص
+
+514
+00:38:16,420 --> 00:38:20,260
+هذا اللي اتعرض للحرق ل ال medical treatment بحاوله
+
+515
+00:38:20,260 --> 00:38:27,000
+على العيادة او للطبيب بحيث انه يشوف علاج للجرح او
+
+516
+00:38:27,000 --> 00:38:32,520
+للحرق، تمام؟ال chemical burn of the skin لو انا
+
+517
+00:38:32,520 --> 00:38:37,320
+احترقت بفعل مادة كيميائية و ليس بفعل الحرارة ايش
+
+518
+00:38:37,320 --> 00:38:41,300
+بدي اعمل اول حاجة بدي اخسل المكان بكمية كبيرة من
+
+519
+00:38:41,300 --> 00:38:45,260
+الماء بعدين بدي اعمل ال neutralizing لالمادة
+
+520
+00:38:45,260 --> 00:38:48,920
+الكيميائية يعني انا لو احترقت ب acid بدي اعمل ال
+
+521
+00:38:48,920 --> 00:38:52,940
+neutralization يعني بدي اضيف ع مكان الحارق sodium
+
+522
+00:38:52,940 --> 00:38:55,920
+bicarbonate عشان ال sodium bicarbonate هي قاعدة
+
+523
+00:38:56,390 --> 00:38:59,710
+هتعادل ال acid وبالتالي تخفف من آثار ال damage
+
+524
+00:38:59,710 --> 00:39:05,110
+اللى ممكن يسببليها وإذا انا احترقت بمادة alkaline
+
+525
+00:39:05,110 --> 00:39:08,630
+بدي اضيف اعمل neutralization في boric acid powder
+
+526
+00:39:08,630 --> 00:39:14,850
+عشان هيك حكينا ان صندوق الاسعافات
+
+527
+00:39:14,850 --> 00:39:17,990
+الأولية لازم يكون في sodium bicarbonate و boric
+
+528
+00:39:17,990 --> 00:39:26,320
+acid تمام؟ طيبلو المادة كيميائية فاتت للعين إيش
+
+529
+00:39:26,320 --> 00:39:31,120
+بدي أعمل؟ حكينا قولنا إنه بدي أخسلها بالماء الجاري
+
+530
+00:39:31,120 --> 00:39:35,060
+لمدة ربيع ساعة و أعمل ال neutralize للمادة
+
+531
+00:39:35,060 --> 00:39:38,520
+الكيميائية هذه طبعا إذا كانت المادة الكيميا acid
+
+532
+00:39:38,520 --> 00:39:42,700
+اللي فاتت للعين بدي أستخدم عشان أعادلها و أقلل من
+
+533
+00:39:42,700 --> 00:39:47,280
+تأثيرها بدي أستخدم 5% Sodium Bicarbonate إذا كانت
+
+534
+00:39:47,280 --> 00:39:52,370
+alkaline بدي أستخدم 5% Acetic Acidimmediately seek
+
+535
+00:39:52,370 --> 00:39:56,210
+medical attention ع طول بعلم الطبيب او العيادة انه
+
+536
+00:39:56,210 --> 00:40:00,270
+صار في عندنا حادث شخص اتعرضت يعني اتقشف في عينه
+
+537
+00:40:00,270 --> 00:40:03,430
+مادة كيميائية و احنا عملنا له ال first aid و عليكم
+
+538
+00:40:03,430 --> 00:40:04,050
+اتكمله
+
+539
+00:40:06,680 --> 00:40:11,460
+swallowing an acid or base يعني ابتلاع مادة فيها
+
+540
+00:40:11,460 --> 00:40:15,740
+حمض أو قاعدة، إيش بدنا نعمل؟ أول حاجة immediately
+
+541
+00:40:15,740 --> 00:40:19,060
+rinse the mouth with water يعني أول حاجة بدي أغمر
+
+542
+00:40:19,060 --> 00:40:23,810
+الماء، أخليه مضمض يعني أكتر من مرةبالماء و بعدين
+
+543
+00:40:23,810 --> 00:40:28,970
+بروح اذا كان هو اطلع acid بدي اعمله neutralization
+
+544
+00:40:28,970 --> 00:40:33,710
+فيه باس يعني في 8% مغنيسيوم هيدروكسيد عشان هيك
+
+545
+00:40:33,710 --> 00:40:38,510
+المغنيسيوم هيدروكسيد من احد مكونات ال first aid
+
+546
+00:40:38,510 --> 00:40:43,950
+box تمام؟أو ممكن أخليه يشرب حليب milk of magnesia
+
+547
+00:40:43,950 --> 00:40:48,350
+تمام؟ عشان يعادل ال acid اللي هو ابطلعه إذا كان
+
+548
+00:40:48,350 --> 00:40:52,910
+الشخص ابطلع alkaline معناها لازم يعادله إياها ب
+
+549
+00:40:52,910 --> 00:40:57,710
+acid بعطيه lemon juice أو 5% acetic acid drink
+
+550
+00:40:57,710 --> 00:41:02,250
+three or four cups of water لازم يشرب ميه كتير
+
+551
+00:41:02,250 --> 00:41:05,330
+عشان يعمل dilution لهذا ال acid و ال base اللي ..
+
+552
+00:41:05,330 --> 00:41:09,210
+اللي ابطلعهو seek medical attention بوجهه للراعية
+
+553
+00:41:09,210 --> 00:41:13,070
+الصحية و بعلم الاعيادة او الدكتور في ان هو صار في
+
+554
+00:41:13,070 --> 00:41:18,310
+عندنا حادث لحتىيحطوه تحت المراقبة لما الشخص يبطلع
+
+555
+00:41:18,310 --> 00:41:21,770
+acid أو alkaline don't encourage vomiting
+
+556
+00:41:21,770 --> 00:41:27,010
+مابنخلوهوش يستفرغ ليش؟ لأنه هالجيت احنا النسيج تبع
+
+557
+00:41:27,010 --> 00:41:32,150
+البلعوم والحنجرة والمريق واتقصانا للمعدة هذا عبارة
+
+558
+00:41:32,150 --> 00:41:37,410
+.. النسيج هذا احترق أول مرة لما .. لما لمس ال acid
+
+559
+00:41:37,410 --> 00:41:40,930
+أو ال alkaline اللي احنا ابطلعناه تمام؟ لما نيجي
+
+560
+00:41:40,930 --> 00:41:45,100
+نعمل vomitingمعناها هيبدو يحترق مرة تانية يعني انا
+
+561
+00:41:45,100 --> 00:41:48,080
+هيك بزود ال damage كمية ال tissue اللي بيصيرلها
+
+562
+00:41:48,080 --> 00:41:51,460
+damage بسبب تعرضها لل acid و ال alkaline بتكون
+
+563
+00:41:51,460 --> 00:41:55,220
+كتير كبيرة اذا انا بقدر الشخص ما بفيده اذا هتخليته
+
+564
+00:41:55,220 --> 00:42:00,460
+يستفرغ فالافضل انه انا اخليه يغسل فمه نعادل المادة
+
+565
+00:42:00,460 --> 00:42:05,440
+اللي أخدها يخليه يشرب مي لحتى يعمل dilution و نحطه
+
+566
+00:42:05,440 --> 00:42:06,220
+تحت المراقبة
+
+567
+00:42:08,730 --> 00:42:12,730
+إذا ابتلع الشخص أي مادة سامة أخرى swallowing of
+
+568
+00:42:12,730 --> 00:42:18,050
+other poisonous chemical طبعا لازم يخسل ال .. فمه
+
+569
+00:42:18,050 --> 00:42:22,230
+بالماء جيدا و طبعا تبع ال .. المادة الكيميائية هذه
+
+570
+00:42:22,230 --> 00:42:25,030
+بستخدم ال antidote تبعها إيش يعني ال antidote يعني
+
+571
+00:42:25,030 --> 00:42:29,550
+literally neutralizing agent تبعها يعني إذا كانت
+
+572
+00:42:29,550 --> 00:42:33,830
+acid بنشوف هو إيش ممكن .. هاي هنا نرجع تاني إيش
+
+573
+00:42:33,830 --> 00:42:38,560
+ابتلع acid؟إيش بنخلق .. بنعمللهم ال neutralization
+
+574
+00:42:38,560 --> 00:42:44,020
+بيه مغنيسيم و hydroxide أو مغنيسية؟ إذا ابتلع مادة
+
+575
+00:42:44,020 --> 00:42:48,480
+alkaline بنعطيه lemon juice أو 5% acetic acid بس
+
+576
+00:42:48,480 --> 00:42:52,000
+هنا الفرق إيش؟ إن المادة سامة فإيش بدي أنا أخد
+
+577
+00:42:52,000 --> 00:42:56,760
+إجراءات تانية؟ على طول بدي أوديه على ال .. ال
+
+578
+00:42:56,760 --> 00:42:59,660
+chemical .. على ال physicalأو sorry يعني ال
+
+579
+00:42:59,660 --> 00:43:05,040
+physician على الدكتور لحتى يعني يعطوه علاج أو
+
+580
+00:43:05,040 --> 00:43:09,920
+يعطوه يعملوله غسيل المعدة أو أي إجراء لحتى يخلصه
+
+581
+00:43:09,920 --> 00:43:13,760
+من أثر المادة السامة هاي اللي ابتلعها Swallowing
+
+582
+00:43:13,760 --> 00:43:18,200
+of infected material إذا .. إذا ابتلع محلول فيه
+
+583
+00:43:18,200 --> 00:43:21,260
+بكتيريا أو فطريات أو أي مادة معضية infection ممكن
+
+584
+00:43:21,260 --> 00:43:25,780
+تعمله infection على طول بروح ب .. اوجهه لطبيب هو
+
+585
+00:43:25,780 --> 00:43:32,280
+اللي بيتعامل معاهتمام؟ إذا الشخص تعرض ل electric
+
+586
+00:43:32,280 --> 00:43:37,340
+shock يعني صدمة كهربائية بدي أ .. أول إشي بدي أطفل
+
+587
+00:43:37,340 --> 00:43:41,620
+كهربا from the mains من المصدر الرئيسي بدي أنزل
+
+588
+00:43:41,620 --> 00:43:46,020
+القاطع تمام؟ و إذا الشخص صار له collapse يعني أغمى
+
+589
+00:43:46,020 --> 00:43:51,150
+عليه أو فقد وعيه أو غيبعلى طول بدي أجيب .. بدي
+
+590
+00:43:51,150 --> 00:43:56,070
+أسأل الطبيب أو ممرض أو أي حد ييجي يسعفه و أتأكد
+
+591
+00:43:56,070 --> 00:44:02,290
+أنه مجرد تنفس عنده مفتوح أرفع رجلي لفوق أحاول أنه
+
+592
+00:44:02,290 --> 00:44:06,390
+أخل .. يعني أرفع رأس .. يعني أنزل رأس لتحت و رقبته
+
+593
+00:44:06,390 --> 00:44:10,500
+هيك خليها ..عالية شوية علشان انه اتأكد انه مجرد
+
+594
+00:44:10,500 --> 00:44:18,220
+تنافس عنده فاتح و طبعا حاول انه اذا كان الوضع صعب
+
+595
+00:44:18,220 --> 00:44:23,660
+على الآخر انه نعطيه انعاش قلب الرؤى.طيب كيف أنا
+
+596
+00:44:23,660 --> 00:44:26,320
+بدي حافظ على ال .. نظافة المختبر laboratory
+
+597
+00:44:26,320 --> 00:44:30,640
+hygiene ال hygiene يعني نظافة never eat, never
+
+598
+00:44:30,640 --> 00:44:33,720
+drink, never smoke يعني لا تاكلوا ولا تشربوا ولا
+
+599
+00:44:33,720 --> 00:44:36,820
+تدخنوا داخل المختبرات طبعا يعني الدنيا مش مستهلة
+
+600
+00:44:36,820 --> 00:44:42,720
+نجودي ندخن علشان حاجات رايحةnever apply cosmetics
+
+601
+00:44:42,720 --> 00:44:47,800
+ماحوتش ما .. ما .. ماسحق تجميل احنا حلوين رباني
+
+602
+00:44:47,800 --> 00:44:51,800
+never touch your face or mouth or eyes ماحاولش ان
+
+603
+00:44:51,800 --> 00:44:54,900
+انا ألمس .. أضل ألمس الوجه و الأيدين و ال eyes
+
+604
+00:44:54,900 --> 00:44:58,700
+بتلاحظوا احنا هنا نلقيت ما ..مع علامات ال .. او
+
+605
+00:44:58,700 --> 00:45:02,680
+sorry مع تحضيرات للوقاية من virus corona بنضلنا
+
+606
+00:45:02,680 --> 00:45:05,780
+نحكي انه يا جماعة الخير اغسلوا أدركوا منيه،
+
+607
+00:45:05,780 --> 00:45:09,600
+ماتلامسش وجهك، أنفك، عيناك، هذه برضه اجراءات
+
+608
+00:45:09,600 --> 00:45:13,160
+وقائية لكتير أمراض فبالتالي don't touch your face,
+
+609
+00:45:13,220 --> 00:45:17,280
+mouth, eyesملاحظ انهش الطالبة و هي لابسة ال gloves
+
+610
+00:45:17,280 --> 00:45:21,180
+بتنصدم هيك و بتتفاجأ هي و زملتها فعلى طول بتروح
+
+611
+00:45:21,180 --> 00:45:24,720
+اشحط ايدها على وجهها او على ثمها وهذه طبعا علامات
+
+612
+00:45:24,720 --> 00:45:30,340
+الدهشة فرجاء لا تندهشوا علشان انه ما تحافظوا على
+
+613
+00:45:30,340 --> 00:45:34,460
+ال hygiene تبعكم never suck pens or chew pencil
+
+614
+00:45:34,760 --> 00:45:39,260
+يعني ما تحاوليش أنك انت تفطي الأقلام الحبر أو
+
+615
+00:45:39,260 --> 00:45:43,600
+الرصاص في فمك و always wash your hands اغسل إيدك
+
+616
+00:45:43,600 --> 00:45:47,880
+باستمرار داخل .. اذا كنت في المختبر طبعا إذا كان
+
+617
+00:45:47,880 --> 00:45:52,800
+عندك allergyحساسية من أي مادة من .. في داخل
+
+618
+00:45:52,800 --> 00:45:56,240
+المختبر لازم تبلغي ال supervisor أو المدرس يعني
+
+619
+00:45:56,240 --> 00:45:59,300
+حاول .. لاحظتي أنك أنت بس تلبسي ال gloves بس
+
+620
+00:45:59,300 --> 00:46:03,480
+تتحسسي خبريني إذا شمتي مادة كيميائية معينة أو
+
+621
+00:46:03,480 --> 00:46:07,200
+حسيتي حالك اختنقتي أو تعبتي أو .. او اتحسستي منها
+
+622
+00:46:07,200 --> 00:46:10,660
+برضه كمان بلغيني لحتى أحاول أن أنا أبعدك عنها أو
+
+623
+00:46:10,660 --> 00:46:14,850
+أجيبلك بدائلطيب ال waste material حكينا أنه
+
+624
+00:46:14,850 --> 00:46:18,690
+الفضلات لازم كل جزء من الفضلات يكون موجود يعني ان
+
+625
+00:46:18,690 --> 00:46:23,850
+ال .. ان تخلص منه بطريقة safely تمامها dispose of
+
+626
+00:46:23,850 --> 00:46:28,210
+waste lab material safely، تخلص منه بأمان، كيف؟
+
+627
+00:46:28,210 --> 00:46:31,830
+يعني والله المواد السائلة بتخلص منها في .. داخل
+
+628
+00:46:31,830 --> 00:46:37,970
+زجاجاتsolvents and oils must be segregated لازم
+
+629
+00:46:37,970 --> 00:46:43,930
+انا اجمعها داخل ايه؟ bottles تمام؟ اذا كان ال ..
+
+630
+00:46:43,930 --> 00:46:49,790
+المواد مشعة بدي احطها في صناديق و اتلفها بطريقة
+
+631
+00:46:49,790 --> 00:46:53,990
+معينة اذا كانت infectant ممكن تعمل .. تنقل عدوة
+
+632
+00:46:53,990 --> 00:46:59,100
+لازم اعقمها و بعدين اتلفهازي إذا كانت مواعد حادة
+
+633
+00:46:59,100 --> 00:47:03,720
+لازم أحطها في ال sharp box فبالتالي كل إشي لازم
+
+634
+00:47:03,720 --> 00:47:08,720
+يطلق بالطريقة الصحيحة لحتى أتخلص منه بأقل الأضرار
+
+635
+00:47:08,720 --> 00:47:18,300
+و طبعا مافعش أنا أتخلص منه بطريقة غير مسئولة أو
+
+636
+00:47:18,300 --> 00:47:23,160
+بطريقة بدون تعليماتدايما انا المدرس احكيلكم حطي ..
+
+637
+00:47:23,160 --> 00:47:25,940
+اتخلصي من ال needle داخل ال sharp box على طول
+
+638
+00:47:25,940 --> 00:47:29,000
+بتخلصي .. بتخلصي منها بال sharp box حكيتلكم انه
+
+639
+00:47:29,000 --> 00:47:32,680
+هيكون في تعليمات لتعامل مع ال waste material في كل
+
+640
+00:47:32,680 --> 00:47:38,180
+تجربة و في كل مختبر احنا بن .. بنجريه لحتى تكون ..
+
+641
+00:47:38,180 --> 00:47:43,440
+تصير بعد هيك عندكم دراية و متدربين منيح انه كيف و
+
+642
+00:47:43,440 --> 00:47:47,760
+وين تتلي في كل مادة بتستخدميها طبعا ..
+
+643
+00:47:50,550 --> 00:47:54,570
+you will need to attend training courses اذا كان
+
+644
+00:47:54,570 --> 00:47:58,590
+انت .. بتحتاج .. بتحتاجي لتدريد اذا كان انت بدك
+
+645
+00:47:58,590 --> 00:48:06,090
+تدومي في .. في ساعات outside the normal hours يعني
+
+646
+00:48:06,090 --> 00:48:09,810
+مثلا في الشفتات الليلية و المساء هذا .. هذا لما
+
+647
+00:48:09,810 --> 00:48:14,420
+تختار .. يعنيتتخرجوا و تشتغلوا فلازم يكون عندك
+
+648
+00:48:14,420 --> 00:48:19,100
+تدريب لإنه طبعا يعني مثلا ال accident في النهار في
+
+649
+00:48:19,100 --> 00:48:23,440
+المنشأة فيها موظفين كتير و فيها ناس ممكن ينقذوكي
+
+650
+00:48:23,440 --> 00:48:27,040
+مش زي لما يحدث معاكي accident في الليل و الناس ..
+
+651
+00:48:27,040 --> 00:48:30,260
+و الموظفين كلهم في بيوتهم و انت بس اللي مناوبة
+
+652
+00:48:30,260 --> 00:48:32,860
+فلازم برضه كمان يعني يكون في عندك تدريب كيف
+
+653
+00:48:32,860 --> 00:48:35,920
+تتعاملي معاه ده أصلا مش كل الشغل بينشغل في الليل
+
+654
+00:48:35,920 --> 00:48:41,160
+يعنيشفتات المسائق بيقللوا فيها نسبة الشغل لحتى
+
+655
+00:48:41,160 --> 00:48:45,580
+يقللوا من الأضرار اللي ممكن تلحق بالأشخاص و طبعا
+
+656
+00:48:45,580 --> 00:48:48,980
+هاي هنا بيحكيلك most experimental work is not
+
+657
+00:48:48,980 --> 00:48:52,100
+permitted يعني معظم الشغل مابيكونش مسموح أنك
+
+658
+00:48:52,100 --> 00:48:56,700
+تشتغليه في الليل و طبعا أكيد مدير المختبر بيشرحلك
+
+659
+00:48:56,700 --> 00:49:00,300
+بالتفصيل إيش مسموح و إيش مش مسموح، إيش عليك و إيش
+
+660
+00:49:00,300 --> 00:49:07,890
+إلكداخل المختبر تمام حكى هنا انه ماتشتغليش don't
+
+661
+00:49:07,890 --> 00:49:11,690
+carry out a new or unfamiliar procedure يعني اي
+
+662
+00:49:11,690 --> 00:49:16,650
+تجربة غريبة ومش مألوفة تشتغليهاش اقتلي الشخص
+
+663
+00:49:16,650 --> 00:49:19,770
+الكيميائي اللي جواكي قبل تفوت المختبر don't guess
+
+664
+00:49:19,770 --> 00:49:25,990
+ماتخمنيش ماتفكريش ماتسرحيش ولا تشطحي انه والله لو
+
+665
+00:49:25,990 --> 00:49:28,960
+ضفت المادة هاي على المادة هاي إيش ممكن أحصليعني مش
+
+666
+00:49:28,960 --> 00:49:32,560
+هنحصل على هالإنجاز الكيميائي الكبير جدما أن أحنا
+
+667
+00:49:32,560 --> 00:49:37,980
+ممكن نعرض حالنا لخطر حكينا هذه ال slide شرحناها
+
+668
+00:49:37,980 --> 00:49:43,380
+قولنا إنه مش لازم أنا أطلق حس الدعابة داخل المختبر
+
+669
+00:49:43,380 --> 00:49:48,340
+و أعمل مجالب زملائي عشان ما أعرض نفسي أو أعرضهم
+
+670
+00:49:48,340 --> 00:49:52,400
+للأذى و برضه كمان في عندنا مخازن بتكون مخزن عليها
+
+671
+00:49:52,400 --> 00:49:56,590
+المواد الكيميائيةممنوع تدخليها إلا بإذن و برضه
+
+672
+00:49:56,590 --> 00:49:59,770
+كمان ممنوع تاخدي أي مادة كيميائية منها برضه إلا
+
+673
+00:49:59,770 --> 00:50:06,790
+بإذن و تلمسي أو تستخدمي أي مادة كيميائية إلا إذا
+
+674
+00:50:06,790 --> 00:50:09,370
+أنا أعطيتك تعليمات فيها و غالبا إنه بيكون اللي
+
+675
+00:50:09,370 --> 00:50:14,190
+متعامل مع المخزن أنا و الفني المختبر حتى كمان أنا
+
+676
+00:50:14,190 --> 00:50:17,810
+بيكون ال access تبعي على هذا المخزن قليل يعني
+
+677
+00:50:17,810 --> 00:50:21,690
+دخولي للمخزن بيكون قليل جداغالبا بيكون في عنا موظف
+
+678
+00:50:21,690 --> 00:50:25,030
+فني مختبر انا دايما بكتبله ورقة بقاش المواد اللي
+
+679
+00:50:25,030 --> 00:50:28,270
+بديها فهو بطلع ليها من المخزن و هيك انا بكون حاميت
+
+680
+00:50:28,270 --> 00:50:31,390
+نفسي فانه كمان برضه الطالبات مش لازم يغامروا
+
+681
+00:50:31,390 --> 00:50:36,910
+يفوتوا للمخزن و يعرضوا حالهم للخطرإذن بدنا نتذكر
+
+682
+00:50:36,910 --> 00:50:40,510
+إنه لازم stay at your work station لازم نضلنا في
+
+683
+00:50:40,510 --> 00:50:44,170
+مكان عملنا طول و عندنا شغال maintain أكلين و .. و
+
+684
+00:50:44,170 --> 00:50:48,910
+a clean work area لازم يكون في عندى مكان عمل نظيف
+
+685
+00:50:48,910 --> 00:50:52,810
+أحافظ عليه نظيف read and follow all directions كل
+
+686
+00:50:52,810 --> 00:51:00,030
+التعليمات أقرأها جيدا و أتبعهاأى spell انسكاب حادث
+
+687
+00:51:00,030 --> 00:51:03,790
+أو injury اتعرضة اتلاقلها او اتعرضلها زملائى لازم
+
+688
+00:51:03,790 --> 00:51:09,830
+تصيرلها reported يعني تكتب تسجل و لازم ا اعمل
+
+689
+00:51:09,830 --> 00:51:14,090
+cleaning انظف او ارفع كل ال equipment بعد ما اخلص
+
+690
+00:51:14,090 --> 00:51:18,190
+من استخدامها و اتخلص من ال waste بالشكل المناسب و
+
+691
+00:51:18,190 --> 00:51:21,630
+هيك بنكون خلصنا علامات السلامة و الأمان اللى داخل
+
+692
+00:51:21,630 --> 00:51:27,450
+المختبر و خلصنا محاضرتنا لليوم طبعاحاولوا أنكم
+
+693
+00:51:27,450 --> 00:51:31,370
+تسمعوها مرة مرتين و تلاتة و مش غلط أنكم تعملوا
+
+694
+00:51:31,370 --> 00:51:35,830
+تلخيص بأهم المعلومات اللي داخلة داخل ال .. اللي
+
+695
+00:51:35,830 --> 00:51:40,170
+يعني اللي داخلة داخل ال chapter هذا و حتى أنه تسهل
+
+696
+00:51:40,170 --> 00:51:45,990
+رجوعكم إليها و تسهل دراستها بشدد يعني أو ب .. ب ..
+
+697
+00:51:45,990 --> 00:51:52,830
+بشدد عليكم أو يعنيأنوه .. أنوه .. الكلمات العربية
+
+698
+00:51:52,830 --> 00:51:57,470
+أحيانا بتضيع مني أنه يعني المواد الكيميائية هذه
+
+699
+00:51:57,470 --> 00:52:00,970
+اللي أنا بدأ أستخدمها لل .. لل neutralization مهمة
+
+700
+00:52:00,970 --> 00:52:04,130
+يعني مثلا في حالة الحرق ال .. ب acid إيش أستخدم له
+
+701
+00:52:04,130 --> 00:52:07,130
+neutralization؟ في حالة ال alkaline إيش أستخدم؟ في
+
+702
+00:52:07,130 --> 00:52:10,210
+حالة الإبتلاء؟ في حالة المادة اللي بت .. بتفوت جوا
+
+703
+00:52:10,210 --> 00:52:13,050
+العين؟ إذا كانت acid أو base إيش أستخدملها؟ ال
+
+704
+00:52:13,050 --> 00:52:16,570
+antidote تبعها أو ال neutralization تبعها تمام؟
+
+705
+00:52:16,570 --> 00:52:19,770
+كلمة antidote أو neutralizing agent نفس المعنى
+
+706
+00:52:20,150 --> 00:52:23,830
+فالمهمين هدولة تحفظوهم حلو لو أنكوا لخصتهم على
+
+707
+00:52:23,830 --> 00:52:28,170
+ورقة واستخدمتوهم يعني راجعتوهم خلال كل دراستكم أو
+
+708
+00:52:28,170 --> 00:52:32,120
+كل ما يكون عليك ال quiz أو إشي ترجعيلهم مش هقلطهيك
+
+709
+00:52:32,120 --> 00:52:36,040
+بنكون خلصنا محاضرتنا لليوم، يعطيكم ألف عافية و
+
+710
+00:52:36,040 --> 00:52:39,340
+شكرا لكم، أي استفسار، أي سؤال بالمحاضرة هذه و
+
+711
+00:52:39,340 --> 00:52:42,600
+المحاضرة السابقة ان شاء الله نلقاكم يوم السبت
+
+712
+00:52:42,600 --> 00:52:47,100
+الساعة ستة على ال zoom لحتى نلتقي منكم أو نستقبل
+
+713
+00:52:47,100 --> 00:52:52,100
+منكم الأسئلة و ناخد إجابات عليها أو يصير حتى نقاش
+
+714
+00:52:52,100 --> 00:52:56,260
+حولها، ممكن أضربلكم أمثلة لحتى أقربلكم الفهمممكن
+
+715
+00:52:56,260 --> 00:52:58,960
+البنت مش فاهمة slide أرجع أشرحلها إياها نفتح ال
+
+716
+00:52:58,960 --> 00:53:03,480
+slide و نرجع نقرأها و نشرحها ممكن أنا يكون عندي
+
+717
+00:53:03,480 --> 00:53:08,400
+أسئلة أو أنتم يكون عندكم أسئلة ف الساعة اللي بناخد
+
+718
+00:53:08,400 --> 00:53:12,180
+فيها مناقشة ال zoom مهمة جدا يا بنات و رجاء ما
+
+719
+00:53:12,180 --> 00:53:15,520
+اتفرطوا فيها و زي ما حكيتلكم إذا حسيت ما فيها ال
+
+720
+00:53:15,520 --> 00:53:20,240
+design رح يكون عليها حضور و غياب هيك أنا بكون خلصت
+
+721
+00:53:20,240 --> 00:53:23,100
+و موفقين بإذن الله شكرا لكم
+
diff --git a/PL9fwy3NUQKwY9jl8xw-bWkVZkWcGGyspd/mJoJF0VELyo.srt b/PL9fwy3NUQKwY9jl8xw-bWkVZkWcGGyspd/mJoJF0VELyo.srt
new file mode 100644
index 0000000000000000000000000000000000000000..599d3ea89349a3079c1f1f2a7f1cb124e2404b73
--- /dev/null
+++ b/PL9fwy3NUQKwY9jl8xw-bWkVZkWcGGyspd/mJoJF0VELyo.srt
@@ -0,0 +1,2786 @@
+1
+00:00:04,390 --> 00:00:07,310
+السلام عليكم ورحمة الله وبركاته. اليوم، إن شاء الله
+
+2
+00:00:07,310 --> 00:00:11,690
+رح نبدأ محاضرة جديدة بعنوان blood components.
+
+3
+00:00:11,690 --> 00:00:16,710
+مكونات الدم. بالبداية، ما هو تعريف الدم؟ ما هو الـ
+
+4
+00:00:16,710 --> 00:00:22,530
+blood؟ تمام. الـ blood أو الدم هو عبارة عن السائل
+
+5
+00:00:22,530 --> 00:00:27,990
+الأحمر، the red fluid that oozes out of your body
+
+6
+00:00:27,990 --> 00:00:31,690
+when you have sustained a cut or deep injury. إذا
+
+7
+00:00:31,690 --> 00:00:40,210
+كان في جرح أو يعني خدش للـ skin أو للأوعية الدموية،
+
+8
+00:00:40,210 --> 00:00:46,690
+تمام، بحيث أنه هذا الجرح أخذ وقت، استغرق وقت قبل
+
+9
+00:00:46,690 --> 00:00:52,490
+التقامه، فأكيد رح يخرج منه سائل أحمر هو الـ blood.
+
+10
+00:00:52,490 --> 00:00:56,860
+تمام، وطبعا احنا أكيد يعني we are familiar with
+
+11
+00:00:56,860 --> 00:01:00,480
+blood. احنا بنعرف الـ blood وشوفنا الـ blood قبل هيك
+
+12
+00:01:00,480 --> 00:01:09,120
+وبنعرف يعني إيش مكوناته، تمام؟ طيب، فالسائل الأحمر
+
+13
+00:01:09,120 --> 00:01:15,310
+هذا هو عبارة عن سائل أكثر كثافة من الماء، وأيضا
+
+14
+00:01:15,310 --> 00:01:20,470
+تقريبا من ثلاث إلى أربع مرات أكثر viscosity، أكثر لزوجة
+
+15
+00:01:20,470 --> 00:01:26,490
+من الماء. أيضا، blood volume، حجم السائل، حجم السائل
+
+16
+00:01:26,490 --> 00:01:31,450
+هذا الأحمر أو الدم، طبعا هو متغير، variable، يختلف من
+
+17
+00:01:31,450 --> 00:01:36,970
+شخص إلى آخر، ولكن هو غالبا بيكون 8% من
+
+18
+00:01:36,970 --> 00:01:42,110
+كتلة جسم الإنسان. يعني احنا طبعا كتلة جسمنا متغيرة،
+
+19
+00:01:42,110 --> 00:01:47,110
+في ناس بيكونوا أطول، أسمك، في ناس بيكونوا نحاف،
+
+20
+00:01:47,110 --> 00:01:51,190
+أقصر، فكتلة جسمهم متغيرة، فبالتالي حجم الدم
+
+21
+00:01:51,190 --> 00:01:56,710
+متغير، ولكن المتعارف عليه أنه هو بيحتل 8%
+
+22
+00:01:56,710 --> 00:01:59,590
+من المية أو يعني بيكون... بياخد 8% من
+
+23
+00:01:59,590 --> 00:02:04,870
+كتلة الجسم. مثلا، لو كان شخص وزنه 50 kilogram،
+
+24
+00:02:04,870 --> 00:02:09,050
+معناها 0.08% يعني 8 من
+
+25
+00:02:09,050 --> 00:02:14,570
+100، تمام؟ ضرب الـ 50 بيكون حجم الـ blood تبعه
+
+26
+00:02:14,570 --> 00:02:18,870
+4 liters. الناس اللي... اللي وزنها أكثر من 50
+
+27
+00:02:18,870 --> 00:02:22,330
+هيكون عندها blood volume أكثر، والناس اللي وزنها
+
+28
+00:02:22,330 --> 00:02:26,650
+أقل من 50 أكيد رح يكون عندهم blood volume أقل،
+
+29
+00:02:26,650 --> 00:02:30,880
+تمام؟ فإذا أعطيناكم وزن في الامتحان وحكيت لكم إنه
+
+30
+00:02:30,880 --> 00:02:34,520
+احسبوا له قداش بيكون عنده blood volume، بس ما عليك
+
+31
+00:02:34,520 --> 00:02:39,600
+إلا أنك أنت بتضرب بـ 8 من 100 أو 8 على 100 في الوزن.
+
+32
+00:02:39,600 --> 00:02:46,260
+تمام؟ طيب، هناك عوامل أخرى تؤثر على حجم الدم أو
+
+33
+00:02:46,260 --> 00:02:49,560
+الـ blood volume، ألا وهي الـ body size. احنا حكينا
+
+34
+00:02:49,560 --> 00:02:53,930
+يعني الـ... كتلة الجسم، حجم الجسم بيأثر على حجم الـ
+
+35
+00:02:53,930 --> 00:02:59,150
+blood. amount of adipocyte، كمان نسبة النسيج الدهني
+
+36
+00:02:59,150 --> 00:03:03,430
+في الجسم برضه كمان بتأثر على حجم الـ blood، والـ
+
+37
+00:03:03,430 --> 00:03:08,490
+electrolyte concentration، قداش تركيز الأملاح
+
+38
+00:03:08,490 --> 00:03:14,440
+والمعادن داخل الجسم أيضا بتأثر على حجم الدم. ولكن الـ
+
+39
+00:03:14,440 --> 00:03:19,300
+... الـ... الحجم اللي قدامه بيتراوح ما بين 4 لترات إلى 6
+
+40
+00:03:19,300 --> 00:03:23,480
+لترات، والـ average volume هو 5 لترات. يعني معظم الناس
+
+41
+00:03:23,480 --> 00:03:27,520
+بنلاحظ أنها هي في نص الـ range واقعة من 4 على 6.
+
+42
+00:03:27,520 --> 00:03:34,220
+المنتصف هو 5 لترات. طيب، لو أنا جيت سحبت عينة دم من
+
+43
+00:03:34,220 --> 00:03:40,420
+وريد، تمام، وحفظيتها في أنبوبة، وهذه الأنبوبة حطيتها
+
+44
+00:03:40,420 --> 00:03:46,140
+في جهاز اسمه centrifuge، تمام، جهاز الطرد المركزي،
+
+45
+00:03:46,140 --> 00:03:52,440
+centrifuge. تمام، هذا الجهاز وظيفته أنه يفصل
+
+46
+00:03:52,440 --> 00:03:58,520
+مكونات الدم. يفصل مكونات الدم. طبعا كيف بيفصلها؟ من
+
+47
+00:03:58,520 --> 00:04:04,300
+اسمه طرد مركزي، يعني الأشياء الثقيلة أو الـ...
+
+48
+00:04:04,300 --> 00:04:09,840
+الوزنها ثقيل بتنزل لتحت في قاع الـ tube، والأشياء
+
+49
+00:04:09,840 --> 00:04:13,920
+الخفيفة أو الـ... أو الـ... المكونات اللي وزنها خفيف
+
+50
+00:04:13,920 --> 00:04:18,440
+بتكون موجودة في the top of the tube، في أعلى الـ
+
+51
+00:04:18,440 --> 00:04:21,950
+tube. زي هنا مثلا، بعد ما أنا أطلعها من جهاز الـ
+
+52
+00:04:21,950 --> 00:04:26,250
+centrifuge، تمام، بلاحظ أنه الدم فصل إلى هذه
+
+53
+00:04:26,250 --> 00:04:30,510
+المكونات. جزء الخلايا اللي موجود في قاع الـ tube،
+
+54
+00:04:30,510 --> 00:04:35,950
+تمام، وجزء السائل اللي موجود في at the top of the
+
+55
+00:04:35,950 --> 00:04:40,790
+tube. طبعا الخلايا أثقل لذلك كانت موجودة في قاع الـ
+
+56
+00:04:40,790 --> 00:04:45,350
+tube، أما السائل فهو أخف، فعشان هيك كان موجود في the
+
+57
+00:04:45,350 --> 00:04:48,770
+top of the tube. طيب، خلينا نشوف إيش السائل، إيش مكو...
+
+58
+00:04:48,770 --> 00:04:54,270
+...إيش اسمه السائل هذا؟ المكون اسمه plasma، تمام؟ والـ
+
+59
+00:04:54,270 --> 00:04:57,370
+مكونات
+
+60
+00:04:57,370 --> 00:05:03,070
+الخلايا. في عندي طبقة بيضاء خفيفة جدا، كخيط رفيع،
+
+61
+00:05:03,070 --> 00:05:07,450
+بسميها الـ buffy coat، اللي بتكون مكونا أو مع...
+
+62
+00:05:07,450 --> 00:05:12,850
+مكونة من خلايا الدم البيضاء، الـ white blood cells، والصفائح
+
+63
+00:05:12,850 --> 00:05:20,240
+الدموية، البلايتليتس. طبعا، وفي عندي الطبقة حمراء اللون
+
+64
+00:05:20,240 --> 00:05:25,160
+بتكون موجودة في القاع، بسميها الـ erythrocytes أو خلايا
+
+65
+00:05:25,160 --> 00:05:30,880
+دم الحمراء، الـ RBCs أو الـ red blood cells. طبعا، طيب، كل
+
+66
+00:05:30,880 --> 00:05:34,760
+من الطبقة البيضاء والطبقة الحمراء بسميها formed
+
+67
+00:05:34,760 --> 00:05:39,180
+elements. هذه هي مكونات الدم. هلأ راح نشوف إيش
+
+68
+00:05:39,180 --> 00:05:43,560
+مكوناته اللي... اللي... اللي يعني متحدثين عنها في
+
+69
+00:05:43,560 --> 00:05:49,020
+الـ slides. هلأ الدم، إذا أنا فصلته، طبعا رح يعطيني
+
+70
+00:05:49,020 --> 00:05:54,860
+three layers، ثلاث طبقات، بشوفهم لما أنا بعمل إيش
+
+71
+00:05:54,860 --> 00:05:59,180
+centrifugation للـ blood. لما أنا بدي يكون عندي دم
+
+72
+00:05:59,180 --> 00:06:04,140
+مفصول بالـ centrifuge، بيكون يعني مفصول إلى ثلاث
+
+73
+00:06:04,140 --> 00:06:10,500
+طبقات. الطبقة الأولى بسميها straw colored liquid أو
+
+74
+00:06:10,500 --> 00:06:16,220
+بسميها plasma. هي سائل ماخذ اللون القشي، straw
+
+75
+00:06:16,220 --> 00:06:21,200
+colored liquid، سائل ماخذ اللون القشي، وبسميه الـ
+
+76
+00:06:21,200 --> 00:06:26,700
+plasma. موجود at the top لإنه خفيف، تمام، وبيحتل
+
+77
+00:06:26,700 --> 00:06:31,780
+تقريبا 55% من حجم الدم. في عندي
+
+78
+00:06:31,780 --> 00:06:37,000
+طبقة بسميها thin cream colored layer، يعني طبقة
+
+79
+00:06:37,000 --> 00:06:43,550
+رفيعة ولونها كريمي، لونها لون أبيض، بسميها الـ buffy
+
+80
+00:06:43,550 --> 00:06:49,090
+coat، وهذه الـ buffy coat هي عبارة عن إيش؟ الـ white
+
+81
+00:06:49,090 --> 00:06:54,290
+blood cells، خلايا الدم البيضاء، and الـ platelets،
+
+82
+00:06:54,290 --> 00:07:00,430
+الصفائح. تمام، وهذه طبعا بيكون حجمها أقل من 1%
+
+83
+00:07:00,430 --> 00:07:04,810
+من المية، تمام؟ أقل من 1% أو تقريبا يعني
+
+84
+00:07:04,810 --> 00:07:09,250
+احنا بنقربها بنحكي 1%. الطبقة الثالثة
+
+85
+00:07:09,250 --> 00:07:14,790
+اللي هي at the bottom، في قاع الـ tube، هي طبقة الـ red
+
+86
+00:07:14,790 --> 00:07:20,210
+blood cells، وهي عبارة عن heavy layer، طبقة ثقيلة،
+
+87
+00:07:20,210 --> 00:07:28,270
+وموجودة في القاع، في قاع الـ tube، وتحتل حجم 45% من
+
+88
+00:07:28,270 --> 00:07:32,520
+الـ whole blood، من عينة الدم اللي أنا سحبتها. طبعا الـ
+
+89
+00:07:32,520 --> 00:07:38,140
+cells، هذه الخلايا بتكون عبارة عن معلقة في داخل
+
+90
+00:07:38,140 --> 00:07:43,820
+سائل، طبعا معلقة داخل سائل، اللي هو الـ plasma. يعني
+
+91
+00:07:45,120 --> 00:07:48,880
+فإذا أنا ممكن أفصل مكونات الدم إما عن طريق الـ
+
+92
+00:07:48,880 --> 00:07:53,300
+filtration، باستخدام filter، أو باستخدام الـ
+
+93
+00:07:53,300 --> 00:07:56,980
+centrifugation، اللي هو باستخدام جهاز centrifuge،
+
+94
+00:07:56,980 --> 00:08:02,220
+أعمله spinning، دوران أو التفاف لهذا الجهاز. مجرد ما
+
+95
+00:08:02,220 --> 00:08:08,100
+أشغله فيه الكهربا بيصير يعمل دوران، التفافات، تمام،
+
+96
+00:08:08,100 --> 00:08:13,240
+سريعة جدا، وبالتالي على إثر هذه التفافات، الأجزاء
+
+97
+00:08:13,240 --> 00:08:18,440
+الثقيلة من السائل بتهبط للأسفل، بتنزل at the bottom
+
+98
+00:08:18,440 --> 00:08:22,460
+of the tube، والأجزاء الخفيفة بتكون موجودة at the
+
+99
+00:08:22,460 --> 00:08:27,220
+top of the tube. إذا، لو أنا اعتبرت أن العينة كلها
+
+100
+00:08:27,220 --> 00:08:31,020
+هاي اللي أنا عبيتها في الـ tube 100%، تمام، لو
+
+101
+00:08:31,020 --> 00:08:35,990
+اعتبرتها أنها كلها 100%، فالـ plasma رح تحتل تقريبا
+
+102
+00:08:35,990 --> 00:08:39,310
+55%، الـ buffy coat يعني 1%
+
+103
+00:08:39,310 --> 00:08:43,330
+أو أقل، والـ erythrocytes 45%.
+
+104
+00:08:43,330 --> 00:08:47,370
+فبالتالي، يعني أنهم تقريبا كلهم بيكونوا
+
+105
+00:08:47,370 --> 00:08:51,230
+100%. ماشي. حكينا أن الـ buffy coat لونها
+
+106
+00:08:51,230 --> 00:08:56,660
+creamy و thin layer، ومكونة من الـ white blood cells والـ
+
+107
+00:08:56,660 --> 00:09:00,740
+platelets. الـ erythrocytes هي الطبقة الحمراء، مكونة من
+
+108
+00:09:00,740 --> 00:09:06,360
+الـ red blood cells، والـ... الـ plasma هي الـ... الـ
+
+109
+00:09:06,360 --> 00:09:10,860
+straw colored liquid، السائل اللي لونه قش اللون،
+
+110
+00:09:10,860 --> 00:09:14,200
+تمام؟ لونه أصفر. طيب، الـ erythrocytes إيش يعني
+
+111
+00:09:14,200 --> 00:09:18,340
+erythrocytes؟ لو أنا جيت قطعتها، erythro لحالها،
+
+112
+00:09:18,340 --> 00:09:23,500
+erythro لحالها، تمام؟ معناها red color، يعني red
+
+113
+00:09:23,500 --> 00:09:28,400
+color. sites، sites معناها خلايا، تمام؟ فـ erythro
+
+114
+00:09:28,400 --> 00:09:33,120
+red، و sites خلايا. إذًا، red cells. إذا أنا أحكي
+
+115
+00:09:33,120 --> 00:09:39,560
+erythrocytes أو أحكي red blood cells، تمام؟ أو RBCs
+
+116
+00:09:39,560 --> 00:09:44,520
+هي نفس المعنى، أنا بقصد خلايا الدم الحمراء. طيب،
+
+117
+00:09:44,520 --> 00:09:51,970
+نيجي للـ plasma. هو أول سائل يعني بيكون هو طبقة من
+
+118
+00:09:51,970 --> 00:09:55,670
+طبقات الـ blood، وبتكون موجودة at the bottom. خلينا
+
+119
+00:09:55,670 --> 00:09:59,030
+نحكي عنها ونشوف إيش هي، إيش هي الـ plasma، من إيش
+
+120
+00:09:59,030 --> 00:10:03,270
+مكونة؟ طبعا، حكينا إنه هي straw colored liquid، و
+
+121
+00:10:03,270 --> 00:10:10,710
+تقريبا بتشغل حوالي 55% من الدم، وطبعا هي عبارة عن الـ
+
+122
+00:10:10,710 --> 00:10:16,390
+transport medium. هي الخلايا الدم بتكون معلقة في هذه
+
+123
+00:10:16,390 --> 00:10:20,770
+الـ plasma. إذا، هي بتتكفل في نقل خلايا الدم من وإلى
+
+124
+00:10:20,770 --> 00:10:25,650
+أعضاء الجسم، تمام؟ إذا، بتنقل الـ blood cells والـ
+
+125
+00:10:25,650 --> 00:10:31,970
+platelets من وإلى أعضاء جسم الإنسان، تمام؟ هذه هي
+
+126
+00:10:31,970 --> 00:10:37,690
+السائل. وطبعا، لما أنا أحكي أن الـ plasma بتحتل نسبة 55
+
+127
+00:10:37,690 --> 00:10:46,330
+% من الدم في العينة أو 55% من الدم في الجسم، فأنا
+
+128
+00:10:46,330 --> 00:10:50,590
+المعلومة واحدة، يعني لأن أنا بما إني أخدت عينة من
+
+129
+00:10:50,590 --> 00:10:55,490
+الدم وكانت هذه العينة ممثلة أو معبرة عن الدم، فلما
+
+130
+00:10:55,490 --> 00:11:00,710
+أجي أفصلها، تمام، راح أفصل نسبة البلازما بالنسبة
+
+131
+00:11:00,710 --> 00:11:04,350
+لهذه العينة هي نفسها نسبة البلازما بالنسبة للدم
+
+132
+00:11:04,350 --> 00:11:09,790
+كله اللي موجود داخل جسم الإنسان، تمام؟ طيب، إذًا، لما
+
+133
+00:11:09,790 --> 00:11:15,130
+أحكي 55% من الـ blood أو من الـ plasma، بنفس المعنى.
+
+134
+00:11:15,130 --> 00:11:20,810
+طيب، ومكونات هاي الـ plasma من إيش مكونات؟ احنا
+
+135
+00:11:20,810 --> 00:11:25,270
+حكينا إنها هي سائل أصفر اللون، ولكن ما هي مكوناته؟
+
+136
+00:11:25,270 --> 00:11:30,290
+أكثر من 90% من هذا السائل هو عبارة عن water، just
+
+137
+00:11:30,290 --> 00:11:35,780
+إيش water؟ الـ plasma هي ماء بالأساس، تمام؟ أكثر من
+
+138
+00:11:35,780 --> 00:11:43,360
+90% منه ماء، ولكن الأقل من 10% اللي باقي هي عبارة
+
+139
+00:11:43,360 --> 00:11:50,920
+عن أيونات، أملاح، تمام، أيونات الصوديوم، الكلوريد، والـ
+
+140
+00:11:50,920 --> 00:11:55,400
+بوتاسيوم، أي شيء... أي أيونات أو أملاح موجودة داخل
+
+141
+00:11:55,400 --> 00:12:00,180
+الجسم. بروتينات زي الـ albumin والـ globulin والـ
+
+142
+00:12:00,180 --> 00:12:05,900
+fibrinogen، بروتينات اللي بتعمل تخ... تخثر للدم،
+
+143
+00:12:05,900 --> 00:12:09,940
+موجودة أيضا في البلازما. الإنزيمات اللي بتقوم
+
+144
+00:12:09,940 --> 00:12:13,980
+بالوظائف المختلفة موجودة داخل البلازما. هي عبارة عن
+
+145
+00:12:13,980 --> 00:12:19,750
+بلازما بروتين، وأيضا dissolved gases أو dissolved
+
+146
+00:12:19,750 --> 00:12:25,930
+gases، تمام؟ غازات مذابة داخل الدم، بتكون موجودة في
+
+147
+00:12:25,930 --> 00:12:29,730
+البلازما زي الـ oxygen، و ثاني أكسيد الكربون، و
+
+148
+00:12:29,730 --> 00:12:34,930
+أيضا موجود... موجود الـ nutrients، يعني مغذيات،
+
+149
+00:12:34,930 --> 00:12:39,890
+أو مصادر للطاقة زي الـ glucose، والأحماض الأمينية، و
+
+150
+00:12:39,890 --> 00:12:43,030
+الأحماض الدهنية. هذه بتكون موجودة أيضا داخل
+
+151
+00:12:43,030 --> 00:12:47,170
+البلازما. بالإضافة إلى الـ circulating tissue
+
+152
+00:12:47,170 --> 00:12:50,850
+products، نواتج اللي بينتجها أو اللي بيطلعها
+
+153
+00:12:50,850 --> 00:12:54,790
+الأعضاء، يعني الـ waste، نواتج الأعضاء، زي اليوريا،
+
+154
+00:12:54,790 --> 00:12:58,330
+كرياتينين، لاكتات. هذه كلها ناتجة من الـ metabolism
+
+155
+00:12:58,330 --> 00:13:02,830
+للأعضاء أو من نواتج لأن الأعضاء عملت function في
+
+156
+00:13:02,830 --> 00:13:07,250
+جسم الإنسان فكان ناتج الـ... الـ function هذه أو ناتج
+
+157
+00:13:07,250 --> 00:13:12,670
+الـ metabolism تبعها ب... مركبات بدنا نتخلص منها،
+
+158
+00:13:12,670 --> 00:13:16,310
+بدها تطلع إما في الـ urine أو في الـ stool، فبالتالي
+
+159
+00:13:16,310 --> 00:13:20,550
+بتكون... إلا تمر عبر الدم، تمام؟ إذًا، الـ tissue
+
+160
+00:13:20,550 --> 00:13:24,630
+products أو الـ tissue waste، نفس الشيء، زي اليوريا،
+
+161
+00:13:24,630 --> 00:13:28,850
+والكرياتينين، واللاكتات. circulating hormones أيضا
+
+162
+00:13:28,850 --> 00:13:33,590
+موجودة في هذه البلازما، هرمونات زي الأنسولين، والـ
+
+163
+00:13:33,590 --> 00:13:37,850
+adrenaline، والـ growth hormone، والهرمونات أخرى،
+
+164
+00:13:37,850 --> 00:13:43,210
+تمام؟ موجودة بتكون داخل البلازما. إذًا، البلازما هي
+
+165
+00:13:43,210 --> 00:13:48,360
+عبارة عن السائل الأصفر اللون أو القشي اللون اللي
+
+166
+00:13:48,360 --> 00:13:53,600
+موجود في at the top of centrifuged blood، تمام؟ و
+
+167
+00:13:53,600 --> 00:14:00,720
+بيشكل 55% من حجم الدم. مكوناته
+
+168
+00:14:00,720 --> 00:14:06,400
+water، ماء، تمام؟ solvent، ماء، و solutes. هذه كلها
+
+169
+00:14:06,400 --> 00:14:10,520
+solutes بسميها... خلينا نوظف التعريفات اللي احنا
+
+170
+00:14:10,520 --> 00:14:15,360
+اتعلمناها في المحاضرة الماضية.
+
+223
+00:18:23,210 --> 00:18:27,850
+إيش؟ generally taken to mean the fluid part of the
+
+224
+00:18:27,850 --> 00:18:31,670
+blood which remains after the blood has coagulated
+
+225
+00:18:31,670 --> 00:18:37,090
+أو clotted هو عبارة عن ال fluid السائل اللي يتبقى
+
+226
+00:18:37,090 --> 00:18:41,830
+بعد إيش أو ينفصل at the top بعد إيش after the
+
+227
+00:18:41,830 --> 00:18:46,290
+blood has coagulated يعني clotted بعد ما الدم يصير
+
+228
+00:18:46,290 --> 00:18:50,850
+له coagulation يصير له clotted فبالتالي ينفصله
+
+229
+00:18:50,850 --> 00:18:55,690
+فالسائل الموجود at the top بسميه serum وإيش
+
+230
+00:18:55,690 --> 00:18:59,390
+معناها؟ مدام صار الدم .. لو الدم صار متخثّر صار
+
+231
+00:18:59,390 --> 00:19:05,840
+متجلّط تمام تحت في at the bottom معناها تم استهلاك
+
+232
+00:19:05,840 --> 00:19:11,280
+بروتينات التخثّر معناها ما فيش فيه fibrinogen هذا
+
+233
+00:19:11,280 --> 00:19:15,480
+السائل القشديّ اللون فبقى اسمه plasma وصار اسمه serum
+
+234
+00:19:18,070 --> 00:19:24,690
+طيب المكوّن الثاني من مكوّنات الدم هي ال red blood
+
+235
+00:19:24,690 --> 00:19:28,890
+cells تمام؟ هي الطبقة الحمراء اللي موجودة at the
+
+236
+00:19:28,890 --> 00:19:32,590
+bottom red blood cells هلّأ ال red blood cells يا
+
+237
+00:19:32,590 --> 00:19:36,770
+بنات لو أنا جيت خلايا دم حمراء ال erythrocyte
+
+238
+00:19:36,770 --> 00:19:43,320
+يعني تمام؟ لو أنا جيت اطلعت عليها نظرة من .. يعني لو
+
+239
+00:19:43,320 --> 00:19:46,620
+أنا حطيتها تحت ال microscope وطلعت عليها من ال ..
+
+240
+00:19:46,620 --> 00:19:50,440
+من أعلى بشوفها عبارة عن discoid قرصية زي شكل
+
+241
+00:19:50,440 --> 00:19:57,340
+الـ CD مدورة تمام؟ وإذا كان أنا جيت وطلعت عليها
+
+242
+00:19:57,340 --> 00:20:02,280
+من الجانب من ال side view نظرة جانبية من جنبها
+
+243
+00:20:02,280 --> 00:20:06,860
+بشوفها biconcave تمام؟ زي الساعة الرملية بيكون
+
+244
+00:20:06,860 --> 00:20:15,540
+شكلها biconcave تمام؟ طيب وبتشغل 99% من خلايا
+
+245
+00:20:15,540 --> 00:20:21,220
+الدم لأن عددها بالملايين خلايا الـ RBCs طبعاً أهم
+
+246
+00:20:21,220 --> 00:20:28,100
+شيء في الـ RBCs أو أهم وظيفة للـ RBCs إنها تحمل الـ
+
+247
+00:20:28,100 --> 00:20:31,520
+red colored hemoglobin البروتين اللي اسمه
+
+248
+00:20:31,520 --> 00:20:36,860
+hemoglobin اللي لونه أحمر تمام؟ عشان هيك هي اكتسبت
+
+249
+00:20:36,860 --> 00:20:41,480
+اللون الأحمر نسبة لوجود فيها .. لوجود أو لاحتوائها
+
+250
+00:20:41,480 --> 00:20:45,360
+على البروتين الأحمر في داخلها، طبعاً؟ فعشان هيك
+
+251
+00:20:45,360 --> 00:20:49,940
+اسمها أخذت لونها أو .. واسمها من لونها والبروتين
+
+252
+00:20:49,940 --> 00:20:56,120
+اللي موجود في داخلها واللي بيشغل تقريباً 70% من
+
+253
+00:20:56,120 --> 00:21:02,170
+حجمها طيب؟ والـ .. هذا الـ hemoglobin هو عبارة عن الـ
+
+254
+00:21:02,170 --> 00:21:10,130
+protein موجود في حلقات iron أو iron .. يعني هو
+
+255
+00:21:10,130 --> 00:21:13,250
+عبارة عن iron-containing protein، موجود فيه
+
+256
+00:21:13,250 --> 00:21:19,530
+حديد، تمام؟ وهذا السبب لأن لونه أحمر وجود ذرات
+
+257
+00:21:19,530 --> 00:21:25,570
+الحديد فيه هي اللي بتكسبه اللون الأحمر طبعاً هو هذا
+
+258
+00:21:25,570 --> 00:21:30,290
+البروتين بيحمل تقريباً 97% من الـ oxygen اللي موجود
+
+259
+00:21:30,290 --> 00:21:34,980
+في أجسامنا، تلاتة في المية .. sorry تلاتة .. تلاتة في
+
+260
+00:21:34,980 --> 00:21:39,440
+المية من الـ oxygen اللي موجود في جسمنا موجودة على
+
+261
+00:21:39,440 --> 00:21:42,960
+بروتينات الـ myoglobin اللي موجودة في العضلات والـ
+
+262
+00:21:42,960 --> 00:21:49,340
+cytochrome اللي موجودة في .. في .. في الخلايا
+
+263
+00:21:49,340 --> 00:21:54,280
+للتنفس، يعني للـ Oxygen أو للـ Energy Production،
+
+264
+00:21:54,280 --> 00:21:58,820
+لإنتاج الطاقة، هتاخدوا في الحيوية دورة Krebs،
+
+265
+00:21:58,820 --> 00:22:03,880
+وهتاخدوا الإنزيمات اللي اسمها الـ Cytochrome،
+
+266
+00:22:03,880 --> 00:22:08,480
+وهتشوفوا إن هي لازم إنها تعطي Electron للـ Oxygen
+
+267
+00:22:08,480 --> 00:22:12,280
+عشان يصير فيه طاقة، إذن هي تحتوي على Oxygen، 3% من
+
+268
+00:22:12,280 --> 00:22:16,000
+الأكسجين موجود على الـ myoglobin والـ cytochrome، أما
+
+269
+00:22:16,000 --> 00:22:20,400
+97% من الأكسجين الموجود في جسمنا موجود محمّل
+
+270
+00:22:20,400 --> 00:22:24,100
+على الـ hemoglobin والـ hemoglobin هو عبارة عن إيش؟
+
+271
+00:22:24,100 --> 00:22:28,380
+هو عبارة عن protein زائد iron، الـ iron أكسب الـ
+
+272
+00:22:28,380 --> 00:22:32,140
+hemoglobin اللون الأحمر، عشان هيك الخلايا اكتسبت
+
+273
+00:22:32,140 --> 00:22:36,700
+اللون الأحمر وصار نسميها ال red blood cells أو الـ
+
+274
+00:22:36,700 --> 00:22:47,630
+erythrocytes أو أحياناً أنا باختصرها بحكيلكم RBCs، رد
+
+275
+00:22:47,630 --> 00:22:49,990
+بلد سيلز
+
+276
+00:22:56,310 --> 00:23:03,350
+طيب ماشي، إذا الخلايا الدم الحمراء وظيفتها حمل
+
+277
+00:23:03,350 --> 00:23:07,310
+الـ hemoglobin يعني oxygen delivery، هي عبارة عن
+
+278
+00:23:07,310 --> 00:23:12,370
+delivery للأكسجين، بتوصل الأكسجين إلى all around
+
+279
+00:23:12,370 --> 00:23:18,030
+the body، ده في كلّ .. في كافة مناطق الجسم، وممكن
+
+280
+00:23:18,030 --> 00:23:23,110
+إنها تحمل ثاني أكسيد الكربون، بتحمل أو تعود محمّلة
+
+281
+00:23:23,110 --> 00:23:26,590
+بثاني أكسيد الكربون من الـ .. من أنسجة الجسم ومن
+
+282
+00:23:26,590 --> 00:23:29,910
+أعضاء الجسم بكمية قليلة طبعاً من ثاني أكسيد الكربون
+
+283
+00:23:29,910 --> 00:23:34,930
+وبتم التخلص منها من الرئتين عن طريق عملية الزفير
+
+284
+00:23:34,930 --> 00:23:42,250
+تمام؟ وبالتالي .. بس إحنا بنتخلص من الكمّية .. من
+
+285
+00:23:42,250 --> 00:23:45,530
+كمية الغاز اللي ما بدنا إياها من ثاني أكسيد الكربون
+
+286
+00:23:45,530 --> 00:23:49,110
+عن طريق عملية الزفير، جزء من ثاني أكسيد الكربون
+
+287
+00:23:49,110 --> 00:23:53,130
+بيكون موجود في البلازما لـ .. حتى يكون blood
+
+288
+00:23:53,130 --> 00:24:00,210
+buffer تمام؟  عندنا كربونات والحمض الكربوني
+
+289
+00:24:00,210 --> 00:24:04,270
+فبالتالي بيكون يعطينا blood buffer وإحنا عرفنا
+
+290
+00:24:04,270 --> 00:24:07,590
+إيش يعني blood buffer وعرفنا من إيش مكوّناته،
+
+291
+00:24:07,590 --> 00:24:11,930
+قلنا weak acid والـ base طبعاً أو الـ salt طبعاً
+
+292
+00:24:11,930 --> 00:24:18,140
+الحمض الكربوني والكربونات، طيب الـ white blood cells
+
+293
+00:24:18,140 --> 00:24:23,860
+الطبقة الثالثة اللي هي بسميها white blood cells، الـ
+
+294
+00:24:23,860 --> 00:24:28,200
+white blood طبعاً يا بنات لها اسم ثاني leukocytes،
+
+295
+00:24:28,200 --> 00:24:31,660
+لو جيت كلمة leuko .. leuko معناها شفافة أو white
+
+296
+00:24:31,660 --> 00:24:36,760
+تمام، و cytes يعني خلايا، إذا هي خلايا الدم البيضاء
+
+297
+00:24:37,030 --> 00:24:40,970
+تمام؟ أو خلايا الدم اللي ما إلها لون، هاي معناها
+
+298
+00:24:40,970 --> 00:24:47,270
+فبالتالي هي عبارة عن جزء مهم وأساسي من الـ blood
+
+299
+00:24:47,270 --> 00:24:51,710
+اللي وظيفته defense against external organisms
+
+300
+00:24:51,710 --> 00:24:58,010
+بيدافع أو بيحمي الجسم من الكائنات أو الأجسام
+
+301
+00:24:58,010 --> 00:25:03,670
+الغريبة اللي تعتبر foreign، غريبة تعتبر pathogen
+
+302
+00:25:03,670 --> 00:25:09,860
+ممرضة لجسم الإنسان، طيب why the white blood cells، خلايا
+
+303
+00:25:09,860 --> 00:25:13,360
+الدم البيضاء ممكن أنا أعطيها لقب ال sanitary
+
+304
+00:25:13,360 --> 00:25:16,480
+engineer؟ إيش يعني sanitary engineer؟ يعني مهندس
+
+305
+00:25:16,480 --> 00:25:22,000
+اللي مسؤول عن عمليات التنظيف والترتيب داخل الجسم،
+
+306
+00:25:22,000 --> 00:25:27,490
+cleaning up، cleaning up what؟ بنظّف إيش؟ بنظّف الجسم
+
+307
+00:25:27,490 --> 00:25:34,750
+من خلايا ميتة، من بقايا الأنسجة، من الأجسام الغريبة
+
+308
+00:25:34,750 --> 00:25:38,770
+المكونات الغريبة اللي بتدخل جسم الإنسان، بيروح
+
+309
+00:25:38,770 --> 00:25:43,430
+بيبلتهمها وبيتخلص منها لحتى يحمي جسم الإنسان من الـ
+
+310
+00:25:43,430 --> 00:25:48,370
+external organisms أو الـ external objects، يا إما
+
+311
+00:25:48,370 --> 00:25:53,600
+أجسام غريبة أو كائنات غريبة، طبعاً؟ طيب، في عندي خمس
+
+312
+00:25:53,600 --> 00:25:57,800
+أنواع من الـ .. الـ white cells أو الـ leukocytes،
+
+313
+00:25:57,800 --> 00:26:02,180
+في عندي neutrophil و eosinophil و basophil، طبعاً؟
+
+314
+00:26:02,180 --> 00:26:06,760
+وفي عندي monocytes و lymphocytes، خمس أنواع، طيب،
+
+315
+00:26:06,760 --> 00:26:10,900
+خلينا نشوف إيش هم هدول خلايا الدم أو إيش أنواعهم،
+
+316
+00:26:10,900 --> 00:26:16,020
+نشوف هنا على خلايا الدم، طبعاً خلايا الدم إحنا كلنا
+
+317
+00:26:16,020 --> 00:26:20,820
+بنعرف إن كلها يتم إنتاجها من إيش؟ من نخاع العظم، من
+
+318
+00:26:20,820 --> 00:26:24,680
+مادة النخاع اللي موجودة في داخل العظام، بتنتج اللي
+
+319
+00:26:24,680 --> 00:26:29,380
+موجودة طبعاً فيها خلايا stem cells، خلايا جذعية، وهذه
+
+320
+00:26:29,380 --> 00:26:35,000
+الخلايا بتتميّز، بتنمو، وبتتميّز، وبتنقسم، وحتى تعطيني
+
+321
+00:26:35,000 --> 00:26:38,480
+red blood cells، خلايا الدم الحمراء، حكيتلكم لو أنا
+
+322
+00:26:38,480 --> 00:26:43,300
+اطلعت عليها من إيش الـ .. الـ .. الـ top view، من الـ
+
+323
+00:26:43,300 --> 00:26:47,180
+.. من .. من فوق لتـ .. يعني من فوق، أطلع عليها نظرة
+
+324
+00:26:47,180 --> 00:26:50,480
+علوية بشوف .. بشوفها زي على هيئة الـ CD، قرصية
+
+325
+00:26:50,480 --> 00:26:55,280
+discoid، أما إذا كانت اطلعت عليها من الجانب
+
+326
+00:26:55,280 --> 00:26:58,780
+بشوفها biconcave زي الساعة الرملية يعني
+
+327
+00:26:58,780 --> 00:27:03,960
+مقعّرة الوجهين تمام؟ أما الـ white blood cells في
+
+328
+00:27:03,960 --> 00:27:10,460
+عندي أنواع basophil و neutrophil وفي monocytes و
+
+329
+00:27:10,460 --> 00:27:14,260
+lymphocytes، طيب هدول الـ .. الـ eosinophil والـ
+
+330
+00:27:14,260 --> 00:27:20,140
+basophil والـ neutrophil بسميها خلايا محببة، خلايا
+
+331
+00:27:20,140 --> 00:27:24,100
+محببة، إيش يعني خلايا محببة؟ مش .. مش المحببة يعني
+
+332
+00:27:24,100 --> 00:27:28,820
+إنها هي الـ favored يعني المفضلة، لأ، خلايا محببة
+
+333
+00:27:28,820 --> 00:27:33,310
+يعني إنّه موجود في الـ cytoplasm تبعها حبيبات، إذا
+
+334
+00:27:33,310 --> 00:27:37,290
+لاحظتوا هنا موجود في الـ cytoplasm تبعها حبيبات، عشان
+
+335
+00:27:37,290 --> 00:27:42,350
+هيك بسميها خلايا محببة، أما الخلايا الغير محببة
+
+336
+00:27:42,350 --> 00:27:46,090
+يعني إنّ الـ cytoplasm تبعها ما فيه حبيبات أو
+
+337
+00:27:46,090 --> 00:27:50,190
+ما بيظهر فيها حبيبات تحت المايكروسكوب، فبسميها
+
+338
+00:27:50,190 --> 00:27:54,870
+monocyte و lymphocyte تمام؟ إذا اللي بتنتهي بـcyte
+
+339
+00:27:54,870 --> 00:27:59,700
+lymphocyte و monocyte هي خلايا غير محببة، أما إذا
+
+340
+00:27:59,700 --> 00:28:03,360
+كانت بتنتهي بكلمة phil، eosinophil، basophil،
+
+341
+00:28:03,440 --> 00:28:09,540
+neutrophil فهي خلايا محببة، ولا ننسى أيضاً إنّه
+
+342
+00:28:09,540 --> 00:28:16,460
+من هذا النخاع في عندي خلايا بتنقسم وبتعطيني الـ
+
+343
+00:28:16,460 --> 00:28:22,010
+platelets، الصفائح الدموية، تمام؟ خلينا نشوف هي هنا
+
+344
+00:28:22,010 --> 00:28:27,790
+granulocyte، granulo يعني حبيبات، cyte خلايا، يعني
+
+345
+00:28:27,790 --> 00:28:33,690
+خلايا فيها حبيبات، خلايا محببة، agranulocyte، فإيش
+
+346
+00:28:33,690 --> 00:28:39,000
+موجود في الـ cytoplasm تبعها؟ موجود حبيبات، تمام؟ والـ
+
+347
+00:28:39,000 --> 00:28:43,860
+a-granulocyte، الـ a لما بيجي في بداية الكلمة بيجي
+
+348
+00:28:43,860 --> 00:28:49,340
+للنفي، تمام؟ a-granulocyte يعني خلايا غير محببة
+
+349
+00:28:49,340 --> 00:28:53,580
+يعني الـ cytoplasm تبعها ما فيه حبيبات، فبسميها
+
+350
+00:28:53,580 --> 00:28:59,580
+monocyte و lymphocyte تمام؟ نيجي نشوف هنا، هاي هنا
+
+351
+00:28:59,580 --> 00:29:05,440
+عندنا الـ .. الخلايا المحببة، الـ .. الـ neutrophil والـ
+
+352
+00:29:05,440 --> 00:29:10,640
+eosinophil والـ basophil، شايفين هم خلايا محببة،
+
+353
+00:29:10,640 --> 00:29:16,720
+هي هنا في شكل أوضح، عندي هنا الـ neutrophil، شوفوا هاي
+
+354
+00:29:16,720 --> 00:29:21,500
+هاي، هاي granulocyte اللي هي الـ neutrophil، الـ
+
+355
+00:29:21,500 --> 00:29:26,460
+eosinophil والـ basophil، أما الخلايا الـ
+
+356
+00:29:26,460 --> 00:29:31,360
+a-granulocyte يعني غير محببة هي الـ monocyte والـ
+
+357
+00:29:31,360 --> 00:29:34,780
+lymphocyte، الـ cytoplasm تبعها خالي من الحبيبات
+
+358
+00:29:34,780 --> 00:29:39,020
+طبعاً هذه الحبيبات اللي فيها مواد يعني بتقتل
+
+359
+00:29:39,020 --> 00:29:43,900
+الميكروبات، فبنسميها .. يعني الحبيبات هذه عبارة عن
+
+360
+00:29:43,900 --> 00:29:49,500
+دي فيها يعني العتاد والعدة لخلايا اللي هذه من
+
+361
+00:29:49,500 --> 00:29:56,830
+شأنها إنها بتدافع عن إيش؟ عن جسم الإنسان، تمام؟ طيب
+
+362
+00:29:56,830 --> 00:30:02,250
+لو جيت أنا هنا، عندي عينة blood، لو فصلتها راح
+
+363
+00:30:02,250 --> 00:30:05,330
+ألاحظ إنّ أنا في عندي، إذا الدم غير متجلّط، معناها
+
+364
+00:30:05,330 --> 00:30:07,970
+السائل ده إيش اسمه؟ plasma، يعني بيحتوي على
+
+365
+00:30:07,970 --> 00:30:12,090
+fibrinogen، الـ fibrinogen من إيش مكوّن أو، sorry الـ
+
+366
+00:30:12,090 --> 00:30:16,790
+plasma هي من إيش مكوّنة؟ مكوّنة من ماء بنسبة أكثر
+
+367
+00:30:16,790 --> 00:30:21,670
+من 90% فيها ماء والباقي solutes، تمام، الماء هو الـ
+
+368
+00:30:21,670 --> 00:30:26,030
+solvent والباقي solutes، إيش هي؟ gases غازات، oxygen،
+
+369
+00:30:26,030 --> 00:30:33,190
+ثاني أكسيد الكربون، كربونات، wastes تمام، الفضلات أو
+
+370
+00:30:33,190 --> 00:30:38,630
+الـ products زي الكرياتينين واللاكتات واليوريا،
+
+371
+00:30:38,630 --> 00:30:44,890
+nutrients تمام، مغذيات، وفيتامينات، glucose، amino
+
+372
+00:30:44,890 --> 00:30:50,960
+acids، fatty acids، بروتينات زي الـ .. بروتينات زي
+
+373
+00:30:50,960 --> 00:30:54,660
+الإنزيمات، والبروتينات المختلفة، والبروتينات
+
+374
+00:30:54,660 --> 00:30:58,200
+الناقلة، الـ albumin والـ globulin والـ fibrinogen، و
+
+375
+00:30:58,200 --> 00:31:02,200
+عوامل التجلط، هذه كلها بروتينات، تمام؟ وأيضاً
+
+376
+00:31:02,200 --> 00:31:06,780
+hormones زي الـ growth hormone والـ insulin والـ
+
+377
+00:31:06,780 --> 00:31:09,420
+adrenaline والـ cortisone، هذه كلها عبارة عن
+
+378
+00:31:09,420 --> 00:31:13,920
+هرمونات بتكون أيضاً موجودة، داخل من مكوّنات البلازما
+
+379
+00:31:13,920 --> 00:31:19,120
+تمام؟ في عندي هنا الطبقة الـ thin layer البيضاء، إيش
+
+380
+00:31:19,120 --> 00:31:22,960
+بسمّي .. من إيش مكوّنة؟ من الـ white blood cells والـ
+
+381
+00:31:22,960 --> 00:31:27,300
+platelets، وبسميها buffy coat تمام؟ اللي هو الطبقة
+
+382
+00:31:27,300 --> 00:31:31,540
+الـ .. أو الـ .. الـ coat الأبيض، تمام؟ الـ red blood
+
+383
+00:31:31,540 --> 00:31:34,340
+cells هي الجزء الثقيل اللي موجود at the bottom
+
+384
+00:31:34,340 --> 00:31:38,360
+وبنعرف إيش يعني red blood cells، هي خلايا دم حمراء
+
+385
+00:31:38,360 --> 00:31:42,510
+اللي بتنقل الـ oxygen، وظيفتها oxygen delivery، تمام،
+
+386
+00:31:42,510 --> 00:31:46,210
+وظيفتها oxygen .. oxygen delivery، لو كان هذا الدم
+
+387
+00:31:46,210 --> 00:31:52,370
+متخثّر، معناها هنا في عندي clot، خلايا متجلطة والسائل
+
+388
+00:31:52,370 --> 00:31:57,150
+هذا عبارة عن إيش؟ عبارة عن serum، بسميه، بطل اسمه
+
+389
+00:31:57,150 --> 00:32:03,410
+plasma، اسمه serum، طبعاً؟ طيب، إذا كان هذا السائل اسمه
+
+390
+00:32:03,410 --> 00:32:07,890
+serum، يعني الدم تحته متجلّط، يعني تم استهلاك
+
+391
+00:32:07,890 --> 00:32:12,490
+بروتينات التجلّط، عوامل التجلّط، طبعاً؟ إذا ما فيش
+
+392
+00:32:1
+
+445
+00:36:24,860 --> 00:36:28,860
+هي هنا طبعا موجود، هاد الـ mega-karyocytes بتروح..
+
+446
+00:36:28,860 --> 00:36:32,160
+موجودة داخل الـ bone marrow بتروح عن طرفي الـ bone
+
+447
+00:36:32,160 --> 00:36:35,900
+marrow وبيصير الـ cytoplasm تبعها يتفتفت ويعطيني
+
+448
+00:36:35,900 --> 00:36:38,880
+جثيمات صغيرة، هذه الجثيمات الصغيرة بتطلع على الدم
+
+449
+00:36:38,880 --> 00:36:43,220
+من برا و بسميها بلاتليت، صفائح دموية، طبعا هذا يا
+
+450
+00:36:43,220 --> 00:36:46,950
+بنات أنا ما.. مش.. مش طلباكم فيه يعني بس أنا عشان
+
+451
+00:36:46,950 --> 00:36:49,990
+أوضح لكم الفكرة، هذا إن شاء الله بتدرسوه في مساقات
+
+452
+00:36:49,990 --> 00:36:54,510
+متقدمة، لكن أنا زي ما بحكيلكم هذه الخلايا الـ mega-
+
+453
+00:36:54,510 --> 00:36:58,950
+karyocyte تمام؟ الـ mega-karyocyte هاي موجودة داخل
+
+454
+00:36:58,950 --> 00:37:04,110
+البون مارو تمام؟ و أحد مكونات خلايا البون مارو.
+
+455
+00:37:04,110 --> 00:37:09,510
+هذا الـ.. الـ mega-karyocyte بتروح على طرف البون و
+
+456
+00:37:09,510 --> 00:37:12,790
+لأول ما تقترب من طرف البون بيصير الـ cytoplasm
+
+457
+00:37:12,790 --> 00:37:18,110
+تبعها يتفتفت يعطيني جثيمات segments قطع، هذه القطع
+
+458
+00:37:18,110 --> 00:37:21,690
+بسميها بلاتليت، الصفائح الدموية، هذه القطع بسميها إيش؟
+
+459
+00:37:21,690 --> 00:37:26,550
+الصفائح، الصفائح الدموية، إذًا الخلية إيش اسمها؟
+
+460
+00:37:26,550 --> 00:37:31,450
+الخلية الأم للصفائح الدموية اسمها mega-karyocyte
+
+461
+00:37:31,710 --> 00:37:36,710
+موجودة وين؟ داخل البون مارو، مش موجودة في الدم، لكن
+
+462
+00:37:36,710 --> 00:37:42,050
+هي الـ cytoplasm تبعها بيتفتفت داخل البون مارو وبيطلع
+
+463
+00:37:42,050 --> 00:37:46,910
+الفتات.. الفتات هذا أو الجثيمات هادي بيطلع للـ
+
+464
+00:37:46,910 --> 00:37:52,490
+دم وعبارة عن إيش؟ بلاتليت، صفائح دموية طبعا
+
+465
+00:37:52,490 --> 00:37:55,950
+الصفائح الدموية كلنا بنعرف إنها الها دور وين في
+
+466
+00:37:55,950 --> 00:38:01,820
+التخثر، play a part in the clotting of blood، الها دور
+
+467
+00:38:01,820 --> 00:38:05,820
+في الـ clotting في التخثر للدم، طب خلينا نشوف إيش
+
+468
+00:38:05,820 --> 00:38:10,920
+دورها في التخثر؟ هلأ هاي القصة هادي story تمام عن
+
+469
+00:38:10,920 --> 00:38:14,840
+الـ blood clotting و دور البلازما و دور البلاتليت في
+
+470
+00:38:14,840 --> 00:38:20,600
+الـ clotting تمام؟ هلأ لنفرض if a damage occurs to a
+
+471
+00:38:20,600 --> 00:38:26,120
+blood vessel أو circuit.. فإذا صار في عندي خدش أو
+
+472
+00:38:26,120 --> 00:38:31,140
+damage أو جرح أو cut أو injury للـ blood vessel
+
+473
+00:38:31,140 --> 00:38:34,440
+تمام؟ يعني مثلا لو أنت جرحت أصبعك، ما هذا صار فيه
+
+474
+00:38:34,440 --> 00:38:40,580
+تهتك للأوعية دموية صح؟ عشان هيك بينزل blood تمام؟
+
+475
+00:38:40,580 --> 00:38:43,460
+فبالتالي إذا صار في عندي damage لهذا الـ blood
+
+476
+00:38:43,460 --> 00:38:49,220
+vessel فبجيناها الـ circulating platelet الصفائح
+
+477
+00:38:49,220 --> 00:38:53,680
+الدموية اللي بتمشي في الـ circulation في الدورة
+
+478
+00:38:53,680 --> 00:38:58,400
+الدموية تمام؟ بتروح على طول على منطقة الجرح
+
+479
+00:38:58,400 --> 00:39:03,780
+بيصيرلها trapped at the injury site بتعلق و بتكون
+
+480
+00:39:03,780 --> 00:39:09,450
+موجودة، بتلتصق بالـ injury site في مكان الجرح، طبعا
+
+481
+00:39:09,450 --> 00:39:14,050
+هذه الـ platelet بتتراكم و بتعمل حاجة اسمها platelet
+
+482
+00:39:14,050 --> 00:39:19,830
+plug، طبعا بترتبط مع بعض ارتباط وثيق و بتعطيني جلطة
+
+483
+00:39:19,830 --> 00:39:27,040
+أو يعني plug ابتدائية اسمها platelet plug، طبعا طيب
+
+484
+00:39:27,040 --> 00:39:31,380
+هذه الخلايا، الصفائح الدموية اللي تراكمت وارتبطت مع
+
+485
+00:39:31,380 --> 00:39:35,360
+بعضها البعض و سكرت مكان الجروح بسميها platelet
+
+486
+00:39:35,360 --> 00:39:40,060
+plug، طبعا بتغير شكل الـ platelet لما بترتبط على
+
+487
+00:39:40,060 --> 00:39:46,040
+مكان الجروح و بيتغير السطح تبعها، فبيصير هذا السطح
+
+488
+00:39:46,040 --> 00:39:52,670
+مناسب لحتى إنه بروتينات التخثر اللي موجودة في الـ
+
+489
+00:39:52,670 --> 00:39:57,930
+plasma، في الدم، تمام؟ إنها.. إذا لمست سطح
+
+490
+00:39:57,930 --> 00:40:01,930
+البلاتليت تتنشط، هي بتكون موجودة طبعا بروتينات
+
+491
+00:40:01,930 --> 00:40:07,950
+التخثر بتكون موجودة، بتسير في الدم خاملة، غير ناشطة،
+
+492
+00:40:07,950 --> 00:40:13,560
+لكن إذا لامست سطح البلاتليت، الـ plug، سطح البلاتليت
+
+493
+00:40:13,560 --> 00:40:19,640
+اللي تراكمت على الـ injury site تمام و اتغير شكلها
+
+494
+00:40:19,640 --> 00:40:24,820
+و اتغير مكونات سطحها، فهذه البروتينات إذا لامست هذا
+
+495
+00:40:24,820 --> 00:40:30,460
+السطح فبتتحول من خاملة إلى ناشطة، طبعا يا بنات أنا
+
+496
+00:40:30,460 --> 00:40:34,180
+كم protein عندي؟ طبعا هي سلسلة من البروتينات مش
+
+497
+00:40:34,180 --> 00:40:37,720
+البروتين ولا اتنين ولا تلاتة، مش عامل ولا عاملين
+
+498
+00:40:37,720 --> 00:40:43,680
+ولا تلاتة للتخثر، إن عندي عوامل للتخثر ما يقارب من
+
+499
+00:40:43,680 --> 00:40:46,660
+إثنا عشر لأربعة عشر، هتاخدوهم بالتفاصيل، يعني أنا ما بدي
+
+500
+00:40:46,660 --> 00:40:51,260
+أفوت لكم بتفاصيل، هتاخدوهم في مساقات لاحقة، لكن هي
+
+501
+00:40:51,260 --> 00:40:56,380
+عبارة عن سلسلة من البروتينات تمام؟ وهي البروتينات
+
+502
+00:40:58,030 --> 00:41:03,070
+بتتنشط كسلسلة، يعني مثلا أول حاجة العامل رقم 12
+
+503
+00:41:03,070 --> 00:41:11,710
+بنشط 11، 11 طبعا بنشط 9، 9 بنشط 8، 8 بنشط 7، وهكذا، فإذا بيصير سلسلة، فالمحصلة إنه بنصل لعامل رقم
+
+504
+00:41:11,710 --> 00:41:18,290
+2 اللي هو الـ thrombin تمام؟ بيكون هو عبارة عن
+
+505
+00:41:18,290 --> 00:41:22,050
+ثرومبين.. ثرومبينوجين بيتنشط و بيتكون لثرومبين،
+
+506
+00:41:22,050 --> 00:41:25,950
+الـ thrombin هو النوع النشط منه تمام؟ من العامل رقم
+
+507
+00:41:25,950 --> 00:41:29,970
+اثنين، هذا العامل رقم اثنين اللي اسمه ثرومبين بيروح
+
+508
+00:41:29,970 --> 00:41:34,590
+بنشط للعامل رقم واحد اللي هو اسمه فيبرينوجين تمام؟
+
+509
+00:41:39,730 --> 00:41:45,000
+الفيبرينوجين هذا شكله عبارة عن rod-shaped
+
+510
+00:41:45,000 --> 00:41:50,120
+soluble protein، هو protein ذائب وشكله شكل الـ rod،
+
+511
+00:41:50,120 --> 00:41:57,020
+شكله شكل العصايا يعني شكل rod تمام، وموجود في الدم
+
+512
+00:41:57,020 --> 00:42:03,180
+بشكل soluble ذائب تمام، إذا تنشط هذا الـ fibrinogen
+
+513
+00:42:03,840 --> 00:42:09,800
+عبر الـ.. الـ thrombin و اتحول لـ fibrin الشكل النشط
+
+514
+00:42:09,800 --> 00:42:16,200
+منه، فهذا الـ fibrin بيكون insoluble تمام؟ وهذا الـ
+
+515
+00:42:16,200 --> 00:42:20,270
+.. الـ protein بيترسب و بيعمل لي شبكة، بتمسك
+
+516
+00:42:20,270 --> 00:42:24,090
+البروتينات و بتمسك الخلايا، الـ RBCs، بيصيرلها
+
+517
+00:42:24,090 --> 00:42:28,070
+trapped، بتتعلق داخل هذه الشبكة و بيكون إيش؟ اللي
+
+518
+00:42:28,070 --> 00:42:32,070
+هو شكل الجلطة الدموية اللي احنا بنشوفها بالشكل الـ
+
+519
+00:42:32,070 --> 00:42:37,750
+solid، بالشكل الصلب، أصبح تم استهلاك الـ fibrinogen
+
+520
+00:42:37,750 --> 00:42:42,910
+فالدم إذا اتجلط تمام، في الجلطة طبعا تم استهلاك
+
+521
+00:42:42,910 --> 00:42:47,250
+واتحول لـ fibrin وارتبط مع.. مع البلاتليت اللي تراكمت و
+
+522
+00:42:47,250 --> 00:42:52,690
+.. فعملي جلطة، تمام؟ فأصبح السائل اللي.. اللي
+
+523
+00:42:52,690 --> 00:42:57,070
+موجود في الأصل.. ف.. فوق الدم المتجلط معناه إيش؟
+
+524
+00:42:57,070 --> 00:43:02,130
+اسمه serum، ولا يحتوي على fibrinogen ولا يحتوي على
+
+525
+00:43:02,130 --> 00:43:08,600
+عوامل التخثر الأخرى، تمام؟ هذا هو الفرق، طيب، فإذا الـ
+
+526
+00:43:08,600 --> 00:43:12,940
+combined action of a platelet and fibrin web اللي
+
+527
+00:43:12,940 --> 00:43:17,360
+..اللي.. الش.. اللي.. خليني أرجع للـ slide
+
+528
+00:43:17,360 --> 00:43:23,500
+sorry، العمل أو الـ.. الـ.. الـ action، الأثر تبع الـ
+
+529
+00:43:23,500 --> 00:43:28,560
+platelet، الصفائح الدموية و شبكة الـ fibrin، تمام؟
+
+530
+00:43:28,560 --> 00:43:34,120
+هذا معناه إنه بيكون لي clot، هذه الـ clot كفيلة بإنها
+
+531
+00:43:34,120 --> 00:43:38,500
+تمنعني إنه يصير في عندي blood loss في جسم الإنسان
+
+532
+00:43:38,760 --> 00:43:43,760
+لإنها بت.. بتثبت الجلطة و بتسكر مكان الجروح و
+
+533
+00:43:43,760 --> 00:43:49,680
+بتظل موجودة لحد الالتئام، لما بيصير الالتئام للجروح
+
+534
+00:43:49,680 --> 00:43:54,960
+و يصير فيه repair طبعا لـ.. الـ tissue لـ الـ.. الـ
+
+535
+00:43:54,960 --> 00:43:59,800
+blood vessel، طبعا يصير له repair يرجع يعني يتعمر و
+
+536
+00:43:59,800 --> 00:44:06,040
+يشتغل و يصير intact و يرجع سليم، طبعا فهذه لحد ما
+
+537
+00:44:06,040 --> 00:44:11,250
+يصير فيه repair and healing وشفاء، معناه الـ.. الـ
+
+538
+00:44:11,250 --> 00:44:14,810
+..الـ clot هي راح تظل موجودة و راح تظل تمنع لي الـ
+
+539
+00:44:14,810 --> 00:44:18,550
+loss of blood، إذا هذه الـ clot إيش معناها؟ ممكن
+
+540
+00:44:18,550 --> 00:44:23,850
+تكون life saving، يعني بتحمي الحياة، احنا في ناس
+
+541
+00:44:23,850 --> 00:44:27,350
+كتير ممكن إنهم يموتوا بسبب النزيف، بسبب إنهم هم
+
+542
+00:44:27,350 --> 00:44:32,110
+ما عندهمش.. مش امكانية أو الجسم هم مش قادر يوقف النزيف
+
+543
+00:44:32,110 --> 00:44:38,480
+هذا، فالناس اللي عندهم impaired clot formation
+
+544
+00:44:38,480 --> 00:44:41,380
+طبعا the formation of a clot is impaired أو
+
+545
+00:44:41,380 --> 00:44:45,420
+impaired clot formation، نتيجة إنه مشكلة إما في
+
+546
+00:44:45,420 --> 00:44:50,920
+عوامل التخثر أو مشكلة في البلاتليت، هدول طبعا at
+
+547
+00:44:50,920 --> 00:44:53,980
+great risk of bleeding to death، يعني ممكن يكون
+
+548
+00:44:53,980 --> 00:44:58,040
+عندهم خطر نزيف حتى الموت، زي الناس اللي بتعاني من
+
+549
+00:44:58,040 --> 00:45:02,720
+المرض الوراثي الـ hemophilia اللي بيكون فيه نقص في
+
+550
+00:45:02,720 --> 00:45:07,880
+عامل التخثر رقم ثمانية تمام؟ رقم ثمانية لما بي..
+
+551
+00:45:07,880 --> 00:45:13,100
+مهم جدًا العامل هذا في.. في السلسلة التخثر لحد ما
+
+552
+00:45:13,100 --> 00:45:17,260
+يتكون عندي الـ fibrin، إذا هذا العامل مش موجود معناه
+
+553
+00:45:17,260 --> 00:45:21,560
+أنا مش هأحصل على fibrin فبالتالي إن الـ.. أشخاص..
+
+554
+00:45:21,560 --> 00:45:26,050
+فبالتالي الـ.. الـ.. الـ.. الـ platelet plug لحالها
+
+555
+00:45:26,050 --> 00:45:30,750
+اللي اتكونت ما بتقدر إنها تسد الـ.. الـ injury
+
+556
+00:45:30,750 --> 00:45:35,070
+فبالتالي ما بتقدر توقف النزيف، فبالتالي إنه ممكن
+
+557
+00:45:35,070 --> 00:45:37,710
+يصير في عندهم نزيف حتى الـ mouth أحيانا في بعض
+
+558
+00:45:37,710 --> 00:45:42,350
+الجروح، لازم إنه يعني يكون فيه.. طبعا الـ platelet
+
+559
+00:45:42,350 --> 00:45:48,210
+plug بتيجي موجودة بتسد مكان الجرح، ولكن الـ fibrin
+
+560
+00:45:48,210 --> 00:45:52,150
+clot أو الـ fibrin web اللي هي شبكة.. شبكة الـ
+
+561
+00:45:52,150 --> 00:45:56,450
+fibrin بتيجي و بتتراكم على سطح الـ platelet، أصلا هي
+
+562
+00:45:56,450 --> 00:45:59,430
+العوامل اللي بتتنشط على سطح الـ platelet، فبتيجي
+
+563
+00:45:59,430 --> 00:46:05,480
+بتتراكم على سطح الـ platelet فبالتالي بت.. بتكون لي
+
+564
+00:46:05,480 --> 00:46:12,280
+شبكة، و الشبكة هذه غير ذائبة و قوية، فبتدعم و بتقوي
+
+565
+00:46:12,280 --> 00:46:16,480
+الجلطة لحد ما يصير في عندي repair and healing مثل
+
+566
+00:46:16,480 --> 00:46:22,320
+ما حكيناه، طيب، إذا أنواع خلايا الدم، erythrocyte، خلايا
+
+567
+00:46:22,320 --> 00:46:27,100
+الدم الحمراء، discoid at the top side، و biconcave
+
+568
+00:46:27,100 --> 00:46:35,520
+at the side view تمام، وفي عندي خلايا دم حمراء، إذا
+
+569
+00:46:35,520 --> 00:46:39,740
+المسؤولة عن الـ oxygen delivery، في عندي خلايا الدم
+
+570
+00:46:39,740 --> 00:46:43,540
+البيضاء اللي هي عبارة عن lymphocytes، خلايا الـ
+
+571
+00:46:43,540 --> 00:46:47,150
+lymphocytes بأحجامها، خلايا الـ monocytes اللي هي
+
+572
+00:46:47,150 --> 00:46:49,770
+عبارة عن إيش؟ حكينا عن الـ lymphocyte و الـ monocyte
+
+573
+00:46:49,770 --> 00:46:55,050
+حكينا إنهم هم agranulocytes، خلايا الـ basophil و الـ
+
+574
+00:46:55,050 --> 00:46:57,930
+eosinophil و الـ neutrophil، هاي شايفين أنتم
+
+575
+00:46:57,930 --> 00:47:01,270
+الحبيبات واضحة في الـ cytoplasm تبعها، فإذًا هي خلايا
+
+576
+00:47:01,270 --> 00:47:08,410
+granulocytes بسميها، طيب نيجي لإشي مهم اللي هو عبارة
+
+577
+00:47:08,410 --> 00:47:13,890
+عن الـ anticoagulants، كلمة coagulation لحالها هذه
+
+578
+00:47:13,890 --> 00:47:20,150
+coagulants يعني تجلط، تمام؟ anticoagulants يعني
+
+579
+00:47:20,150 --> 00:47:26,650
+عوامل مانعة للتجلط، هلأ احنا في الحصص اللي.. اللي
+
+580
+00:47:26,650 --> 00:47:31,830
+بتجرى لتقييم محتويات الدم، تمام؟ اللي بسميها الـ
+
+581
+00:47:31,830 --> 00:47:35,530
+hematology and coagulation tests أو hematology and
+
+582
+00:47:35,530 --> 00:47:38,670
+coagulation procedures، الحصص اللي بتجرى عليها
+
+583
+00:47:38,670 --> 00:47:42,250
+عينة الدم لحتى أنا أقيم فيها مكونات الدم، إما بدي
+
+584
+00:47:42,250 --> 00:47:45,930
+أعد خلايا الدم أو أشوف نسبة الـ hemoglobin في الدم
+
+585
+00:47:45,930 --> 00:47:49,670
+أو بدي أشوف نسبة عوامل التخثر في الدم أو نشاطها أو
+
+586
+00:47:49,670 --> 00:47:53,710
+تركيزها، فهذه كلها اللي بتقيم لي وظائف الدم تمام؟
+
+587
+00:47:53,880 --> 00:47:58,340
+فبالتالي كلها بتجرى على إيش؟ بتجرى على عينة إما
+
+588
+00:47:58,340 --> 00:48:04,880
+اسمها whole blood يعني دم كامل تمام، أو plasma، أصبح
+
+589
+00:48:04,880 --> 00:48:07,980
+طالما.. طالما إنه أنا بدي أحصل على plasma، إذا
+
+590
+00:48:07,980 --> 00:48:12,700
+لازم الدم اللي أنا بدي أسحبه من المريض اسمه
+
+591
+00:48:12,700 --> 00:48:18,320
+ما يتجلط، عشان أحصل على plasma، طبعا إن الدم.. طبعا
+
+592
+00:48:18,320 --> 00:48:21,720
+الدم احنا لو سحبناه وتركناه في أنبوبة على طول
+
+593
+00:48:21,720 --> 00:48:25,620
+بيتجلط لحاله، بتنشط.. بتنشط عوامل التخثر و بتجلط
+
+594
+00:48:25,620 --> 00:48:30,400
+طبعا؟ طيب، ف.. لكن أنا بدي أسحبه و بدي أحصل على
+
+595
+00:48:30,400 --> 00:48:35,460
+plasma، إذا لازم أضيف له مادة تمنع تجلطة علشان أنا
+
+596
+00:48:35,460 --> 00:48:41,360
+.. يعني الـ.. أظل محافظة على قوامه السائل ولما
+
+597
+00:48:41,360 --> 00:48:46,920
+أحصله أحصل عليه plasma تمام؟ طيب، إذا عشان الـ..
+
+598
+00:48:46,920 --> 00:48:50,600
+الفحوصات إما بتجرى على whole blood أو على plasma
+
+599
+00:48:50,600 --> 00:48:54,440
+يعني أنا بدي الدم يضل سائل عشان.. و يضل كامل مخلوط
+
+600
+00:48:54,440 --> 00:48:58,020
+و كامل أو plasma يعني يضل سائل وأنا لما أفصل و
+
+601
+00:48:58,020 --> 00:49:02,580
+أحصل على السائل الأصفر اللي موجود at the top تمام؟
+
+602
+00:49:02,880 --> 00:49:06,700
+فبالتالي الـ blood is withdrawn from the patient،
+
+603
+00:49:06,700 --> 00:49:11,960
+الدم اللي بِسحب من المريض لازم يخلط، يصير له mixed
+
+604
+00:49:11,960 --> 00:49:15,220
+مع إيش؟ مع anticoagulant، أنا بدي أضيف له
+
+605
+00:49:15,220 --> 00:49:20,800
+anticoagulant، مادة أو عامل مانع للتخثر، من كلمة..
+
+606
+00:49:20,800 --> 00:49:25,200
+من كلمة anti يعني مضاد، تمام؟ coagulants يعني
+
+607
+00:49:25,200 --> 00:49:29,460
+تخثر، مضاد للتخثر، عشان إيش أعمل؟ عشان prevent
+
+608
+00:49:29,460 --> 00:49:34,220
+coagulation، أمنع تخثر الدم، فبالتالي أظل محافظة على
+
+609
+00:49:34,220 --> 00:49:38,720
+الدم سائل فبالتالي أقدر أحصل على whole blood تمام
+
+610
+00:49:38,720 --> 00:49:42,540
+و أحصل على plasma، تفيدني في الفحوصات اللي بدي
+
+611
+00:49:42,540 --> 00:49:47,980
+أعملها
+
+667
+00:53:40,300 --> 00:53:44,140
+وفصلته السائل القشي اللي موجود at the top طالما
+
+668
+00:53:44,140 --> 00:53:47,320
+الدم مش متجلط اسمه plasma اذا بينفعني في الفحوصات
+
+669
+00:53:47,320 --> 00:53:54,160
+الهيماتولوجية تمام؟ طيب خلينا نشوف قبل أحكي عن هذه
+
+670
+00:53:54,160 --> 00:53:57,640
+النقطة إيش هي .. كيف شكل الأنابيب اللي أنا بجمع
+
+671
+00:53:57,640 --> 00:54:02,940
+فيها عينة الدم خلينا نشوف هاي هنا الtube of blood
+
+672
+00:54:02,940 --> 00:54:07,340
+هذه طبعا أنابيب مختلفة فيها anticoagulant مختلف
+
+673
+00:54:07,340 --> 00:54:13,220
+تمام؟ ونجمع فيها عينة الدم كل أنبوب بتكون متخصصة
+
+674
+00:54:13,220 --> 00:54:19,120
+لفحص معين مثلا في عندي بيكون سترات في بيكون ال ..
+
+675
+00:54:19,120 --> 00:54:26,720
+heparin  EDTA طبعا لل ESR سترات و هي هنا فيه
+
+676
+00:54:26,720 --> 00:54:30,400
+plain يعني مافيش فيها حاجة خالص طبعا فاضية يعني
+
+677
+00:54:30,400 --> 00:54:33,520
+الدم هيتجلط فيها مدى مافيش فيها anticoagulant الدم
+
+678
+00:54:33,520 --> 00:54:36,680
+هيتجلط فيها ولما أفصلها في ال centrifuge هتعطيني
+
+679
+00:54:36,680 --> 00:54:40,240
+serum مش plasma طبعا اذا هذه هي الأنابيب اللي هي
+
+680
+00:54:40,240 --> 00:54:44,890
+color .. color code يعني كل لون عبارة بيدللي ان
+
+681
+00:54:44,890 --> 00:54:48,810
+محتوياته مختلفة تمام في ال anticoagulant اللي
+
+682
+00:54:48,810 --> 00:54:52,370
+موجود فيه مختلف اما بيكون فيها anticoagulant مختلف
+
+683
+00:54:52,370 --> 00:54:55,570
+أو بيكونش فيها anticoagulant خالص زي البلان هاي
+
+684
+00:54:55,570 --> 00:55:00,050
+الحمرة تمام فبالتالي اما بتفصل لي plasma أو بتفصل لي
+
+685
+00:55:00,050 --> 00:55:04,730
+serum لما أحطها على جهاز ال centrifuge فبالتالي
+
+686
+00:55:04,730 --> 00:55:09,510
+حسب ايش مكوناتها أو ايش محتويات ال tube تمام؟ يا
+
+687
+00:55:09,510 --> 00:55:12,770
+بنات كمية ال anticoagulant اللي موجودة احيانا
+
+688
+00:55:12,770 --> 00:55:18,700
+بتكون يعني  لا تكتت الكارويعني احيانا ما بتشوفوها ..
+
+689
+00:55:18,700 --> 00:55:21,860
+ممكن تشوفيها ك powder أو تشوفيها كقطرات على جدار
+
+690
+00:55:21,860 --> 00:55:25,640
+التيوب و ممكن ما تشوفيها، ما تلاحظيها، لكن هي
+
+691
+00:55:25,640 --> 00:55:29,100
+بيكون موجودة فيها كميات قليلة من ال anticoagulant
+
+692
+00:55:29,100 --> 00:55:33,940
+وبالتالي أيضا ما بيكون معلملي اني أنا أسحب الدم
+
+693
+00:55:33,940 --> 00:55:36,560
+لغاية علامة معينة في التيوب هذه، يعني أنا لو بدي
+
+694
+00:55:36,560 --> 00:55:41,760
+أفضي التيوب داخل أو أفضي blood داخل التيوب، بدي
+
+695
+00:55:41,760 --> 00:55:46,130
+أفضيه عند علامة معينة بحيث انه يظل نسبة الدم لل
+
+696
+00:55:46,130 --> 00:55:48,890
+anticoagulant ثابتة  ثابتة يعني ال anticoagulant
+
+697
+00:55:48,890 --> 00:55:53,250
+هذا ملح بيأثر احيانا على شكل الخلايا بيأثر على
+
+698
+00:55:53,250 --> 00:55:56,370
+المكونات
+
+699
+00:55:56,370 --> 00:56:01,370
+الدم فبالتالي لازم أنا أظل محافظة على النسبة ما
+
+700
+00:56:01,370 --> 00:56:04,830
+بين الدم وعامل ال anticoagulant هذا بحيث اني
+
+701
+00:56:04,830 --> 00:56:08,490
+ما أثر على محتويات الدم وبالتالي ما أحصل على نتائج
+
+702
+00:56:08,490 --> 00:56:12,190
+خاطئة في الفحوصات يعني مثلا هي الأنبوبة لـ tube هذه ال
+
+703
+00:56:12,190 --> 00:56:15,890
+tube ال ESR فيها Sodium Citrate تمام ال .. ال
+
+704
+00:56:15,890 --> 00:56:18,310
+anticoagulant اللي موجود اسمه Sodium Citrate
+
+705
+00:56:18,310 --> 00:56:22,010
+هنشتغل فحص ال ESR و هنحكي عن Sodium Citrate
+
+706
+00:56:22,010 --> 00:56:26,410
+anticoagulant وهيتكرر على ما أسمعكم هذا الاسم
+
+707
+00:56:26,410 --> 00:56:30,920
+كتير Sodium Citrate تمام؟ طيب شايفين العلامة هذه
+
+708
+00:56:30,920 --> 00:56:36,140
+اللي لونها أسود هذه لغاية عندها لازم أنا أعبي blood
+
+709
+00:56:36,140 --> 00:56:40,580
+يعني لغاية هنا أعبي blood عشان ما يكونش كمية ال ..
+
+710
+00:56:40,580 --> 00:56:44,820
+السترات كمية الملح أكثر في الدم فبالتالي تأثر على
+
+711
+00:56:44,820 --> 00:56:49,680
+شكل الخلايا ومكونات الدم أحيانا بيكون اذا ما أعطاني
+
+712
+00:56:49,680 --> 00:56:53,300
+علامة على الجانب ممكن يكون هذا الخط الملون هو هو
+
+713
+00:56:53,300 --> 00:56:57,400
+نفس العلامة تمام؟ هذه شايفين ايش فيها زي الشمع أو
+
+714
+00:56:57,400 --> 00:57:01,820
+زي ال .. ال silicon زي مادة ال silicon جوا؟ هذه
+
+715
+00:57:01,820 --> 00:57:06,080
+بيكون فيها clot activator يعني بتعطيني serum تمام؟
+
+716
+00:57:06,080 --> 00:57:10,220
+بت .. بس ايش .. يعني .. يعني الدم بيتجلط أسرع من
+
+717
+00:57:10,220 --> 00:57:12,640
+هذه هذا الدم .. هذه مافيش فيها حاجة يعني أنا بترك
+
+718
+00:57:12,640 --> 00:57:16,000
+الدم يتجلط على راحتة بعدين لما أفصل في جهاز ال
+
+719
+00:57:16,000 --> 00:57:22,700
+centrifuge بيعطيني serum المكونات انه عبارة عن يعني
+
+720
+00:57:22,700 --> 00:57:27,810
+دم متجلط و فوقها top serum  نفس الشيء بيتجلط
+
+721
+00:57:27,810 --> 00:57:32,610
+الدم ولما أفصل بجهاز ال centrifuge بيعطيني ال
+
+722
+00:57:32,610 --> 00:57:36,990
+serum والدم المتجلط بالأسفل ولكن هنا بيتجلط أسرع
+
+723
+00:57:36,990 --> 00:57:41,230
+بسبب أن هذه مادة ال silicon بقرأ عن clot activator
+
+724
+00:57:41,230 --> 00:57:47,950
+يعني منشطة لحدوث التجلط تمام؟ طيب فإذا بدي اياكم بس
+
+725
+00:57:47,950 --> 00:57:52,790
+تركزوا كتير منيح انه حجم الدم هنا بيكون طبعا في
+
+726
+00:57:52,790 --> 00:57:57,670
+عندي علامة بتكون موجودة لتحدد لي أنا أعبي ال
+
+727
+00:57:57,670 --> 00:58:02,490
+tube هاي ل .. ل .. لغاية  لغاية وين في ال blood
+
+728
+00:58:02,490 --> 00:58:06,150
+تمام؟ اما بيكون علامة على الجانب أو الخط الملون
+
+729
+00:58:06,150 --> 00:58:10,990
+هذا نفسه بيكون هو نفس العلامة طيب خلينا نشوف طيب
+
+730
+00:58:10,990 --> 00:58:16,450
+لو أنا كان في عندي في ال tube تبعتي excessive
+
+731
+00:58:16,450 --> 00:58:20,450
+concentration of EDTA ايش يعني؟ يعني ال EDTA
+
+732
+00:58:20,450 --> 00:58:26,190
+زيادة يعني الدم أنا سحبته أقل من العلامة طبعا؟
+
+733
+00:58:26,190 --> 00:58:30,690
+مدام في عندي ملح كتير هذا هيأثر على شكل خلايا ال
+
+734
+00:58:30,690 --> 00:58:35,370
+RBCs ويعمل لها shrinkage ايش يعني shrinkage؟ يعني
+
+735
+00:58:35,370 --> 00:58:42,150
+الخلايا هتتكرمش هيصير لها كرمشة طبعا؟ وتتقلص
+
+736
+00:58:43,060 --> 00:58:47,200
+فبالتالي هذا هيأثر على الفحوصات الهيماتوكريت هيخليه
+
+737
+00:58:47,200 --> 00:58:54,460
+decreased وهيخلي ال MCHC increased وهيقلل ال ESR
+
+738
+00:58:54,460 --> 00:59:00,120
+تمام؟ كل الفحوصات اللي بتعتمد على حجم الخلايا الدم
+
+739
+00:59:00,120 --> 00:59:03,740
+الحمراء على قياس حجم خلايا الدم الحمراء كلها راح
+
+740
+00:59:03,740 --> 00:59:09,510
+تتأثر decreased hematocrit و increased MCHC و
+
+741
+00:59:09,510 --> 00:59:13,550
+falsely low ESR هذه طبعا ايش معناها؟ هذه نتائج خاطئة
+
+742
+00:59:13,550 --> 00:59:18,130
+أنا حصلت عليها نتيجة اني أنا عبيت ال blood أقل من
+
+743
+00:59:18,130 --> 00:59:21,890
+العلامة داخل التيوب يعني أنا ما حطيت كفاية blood
+
+744
+00:59:21,890 --> 00:59:25,470
+داخل التيوب فبالتالي كمية ال EDTA كانت زيادة
+
+745
+00:59:25,470 --> 00:59:30,000
+بالنسبة ل blood وايضا ال EDTA زيادة ممكن
+
+746
+00:59:30,000 --> 00:59:32,780
+تعمل degenerative changes in white cells يعني
+
+747
+00:59:32,780 --> 00:59:37,480
+ممكن تخلي ال white cells انها هي تتحلل ممكن تعمل لي
+
+748
+00:59:37,480 --> 00:59:43,220
+swelling لل platelets فبالتالي هي ايضا هيخلي لي
+
+749
+00:59:43,220 --> 00:59:46,500
+هيعملي falsely و تخليها طبعا لما انها صار لها
+
+750
+00:59:46,500 --> 00:59:51,220
+swelling  ال platelets لما انها انتفخت ممكن تتكسر ولما
+
+751
+00:59:51,220 --> 00:59:58,170
+أنا قاعدها على الجهاز مفتات ل platelets يعدوا الجهاز
+
+752
+00:59:58,170 --> 01:00:02,870
+ك platelets فبالتالي انه يعمل لي increase in platelet
+
+753
+01:00:02,870 --> 01:00:11,050
+count طبعا؟ طيب Sodium citrate النوع الثاني اللي هو
+
+754
+01:00:11,050 --> 01:00:15,350
+ال الأكثر شيوعا في المختبرات ال
+
+755
+01:00:15,350 --> 01:00:19,330
+hematology هو عبارة عن Sodium citrate طبعا ملح
+
+756
+01:00:19,330 --> 01:00:25,530
+Sodium citrate بيكون اما موجود ك Sodium citrate
+
+757
+01:00:25,530 --> 01:00:30,930
+dehydrate أو Sodium citrate عادي طبعا طيب فبالتالي
+
+758
+01:00:30,930 --> 01:00:34,810
+أو potassium citrate أحيانا فالـ ال sodium
+
+759
+01:00:34,810 --> 01:00:39,150
+citrate هذا دايما أنا بحصل .. يعني طبعا اذا بدي
+
+760
+01:00:39,150 --> 01:00:43,290
+أعمل فحوصات التخثر ال coagulation studies لازم
+
+761
+01:00:43,290 --> 01:00:48,990
+يكون نسبة الدم تسعة أجزاء و ال citrate واحد يعني
+
+762
+01:00:48,990 --> 01:00:56,120
+نسبة ال anticoagulant لل blood واحد لتسعة تمام؟
+
+763
+01:00:56,120 --> 01:01:00,020
+فإذا مهم جدا حكاية العلامة هذه وكمية الدم اللي أنا
+
+764
+01:01:00,020 --> 01:01:03,700
+بدي أفضيه داخل ال tube بعد ما أسحب العينة Sodium
+
+765
+01:01:03,700 --> 01:01:08,200
+citrate prevents coagulation by binding the calcium
+
+766
+01:01:08,200 --> 01:01:12,020
+to the blood in a soluble complex طبعا احنا حكينا
+
+767
+01:01:12,020 --> 01:01:15,660
+انه هذا النوع من ال anticoagulant زي وزي ال EDTA
+
+768
+01:01:15,660 --> 01:01:19,500
+بيمنع التجلط من خلال ارتباطه بال calcium واحنا
+
+769
+01:01:19,500 --> 01:01:24,010
+حكينا ان ال calcium ضروري لتنشيط عوامل التجلط فاذا
+
+770
+01:01:24,010 --> 01:01:27,890
+مافيش calcium معناها عوامل التجلط مش رح تنشط
+
+771
+01:01:27,890 --> 01:01:31,750
+فمافيش clot فبالتالي الدم رح يظل سائل فاذا عشان
+
+772
+01:01:31,750 --> 01:01:35,950
+هيك بسمي هذه المواد anticoagulant مضادات للتجلط
+
+773
+01:01:35,950 --> 01:01:40,460
+موانع للتجلط تروح ترتبط بالكالسيوم وبتمنع التجلط
+
+774
+01:01:40,460 --> 01:01:46,420
+طيب ثانيا الهيبارين نوع ثالث من ال anticoagulant اللي
+
+775
+01:01:46,420 --> 01:01:52,100
+اكثر شيوعا في ال hematology lab ولكن طبعا انا ممكن
+
+776
+01:01:52,100 --> 01:01:58,830
+استخدمها من 15 ل 30 unit per ml تركيزه طبعا ممكن
+
+777
+01:01:58,830 --> 01:02:01,670
+أحيانا يسبب لي clumping لل platelets في ناس عندهم
+
+778
+01:02:01,670 --> 01:02:05,630
+حساسية من ال heparin فبالتالي ممكن أنا اذا ضفت لهم
+
+779
+01:02:05,630 --> 01:02:09,750
+heparin لعيناتهم يصير فيه clumping يصير فيه تجمعات
+
+780
+01:02:09,750 --> 01:02:15,650
+ل platelets و white blood cells فبالتالي هدول
+
+781
+01:02:15,650 --> 01:02:19,970
+الناس أنا اذا لاحظت اني استخدمت ال anticoagulant
+
+782
+01:02:19,970 --> 01:02:23,630
+هذا ال heparin و روحت أعد خلايا ال platelets و أعد
+
+783
+01:02:23,630 --> 01:02:26,610
+ال white blood cells و لقيتها قليلة بدو يجي في بالي ان
+
+784
+01:02:26,610 --> 01:02:29,230
+هو ممكن يكون هذا المريض بيتحسس من الهيبارين
+
+785
+01:02:29,230 --> 01:02:32,750
+فبالتالي عشان هيك صار في عنده خلايا ال .. ال
+
+786
+01:02:32,750 --> 01:02:35,750
+platelets و ال white blood cells صار لها clumping فبالتالي
+
+787
+01:02:35,750 --> 01:02:39,390
+لازم أنا ابدل يعني أسحب له عينة ثانية واحطها في
+
+788
+01:02:39,390 --> 01:02:44,010
+anticoagulant اخر بعدين اعمل count تمام؟ طيب هذه
+
+789
+01:02:44,010 --> 01:02:48,670
+يعني بس يعني من ال limitations أو من عيوب الهيبارين
+
+790
+01:02:49,160 --> 01:02:53,540
+طيب ال heparin بيمنع التجلط أيضا، اذا انا ممكن
+
+791
+01:02:53,540 --> 01:02:57,180
+أحصل على plasma من عينة الدم اللي مضيفة لها هيبارين،
+
+792
+01:02:57,180 --> 01:03:01,860
+ولكن آليته مختلفة، احنا حكينا ال EDTA و
+
+793
+01:03:01,860 --> 01:03:06,840
+ال citrate بيرتبطوا بال calcium، طبعا؟ فبيمنع ال
+
+794
+01:03:06,840 --> 01:03:11,340
+ال .. ال .. تنشيط عوامل التجلط، فبالتالي ما بيصيرش
+
+795
+01:03:11,340 --> 01:03:16,300
+فيه clot طبعا لكن الهيبارين ايش بيروح يعمل؟ بيروح
+
+796
+01:03:16,300 --> 01:03:21,100
+بيشتغل بآلية مختلفة بميكانيزم مختلف الهيبارين
+
+797
+01:03:21,100 --> 01:03:26,340
+بيروح بينشط ال protein اسمه ال antithrombin 3 وهذا
+
+798
+01:03:26,340 --> 01:03:30,420
+ال antithrombin 3 بيروح بيرتبط مع ال thrombin و
+
+799
+01:03:30,420 --> 01:03:34,340
+بيعمله inhibition طبعا فطالما ارتبط مع ال thrombin
+
+800
+01:03:34,340 --> 01:03:38,480
+وعمله inhibition مافيش عندي fibrinogen يتحول ل
+
+801
+01:03:38,480 --> 01:03:42,520
+fibrin فاذا مافيش عندي clot فاذا ال blood هيظل سائل
+
+802
+01:03:42,610 --> 01:03:47,570
+طبعا هذا هو الفارق في الآلية ما بين ال .. ال
+
+803
+01:03:47,570 --> 01:03:51,960
+EDTA والسترات وما بين الهيبارين طيب ال blood
+
+804
+01:03:51,960 --> 01:03:56,980
+collecting tubes طبعا احنا في عندنا عدة انواع من ال
+
+805
+01:03:56,980 --> 01:03:59,780
+tubes اللي هي ال blood collecting tubes هي هنا فيه
+
+806
+01:03:59,780 --> 01:04:02,500
+several types of blood collecting tubes are
+
+807
+01:04:02,500 --> 01:04:08,560
+available وطبعا حكينا ان هي ال .. ال .. ممكن انا
+
+808
+01:04:08,560 --> 01:04:11,760
+اميزها discriminated by their top color حكينا
+
+809
+01:04:11,760 --> 01:04:16,610
+انه لها color code كل لون الغطاية أو اللون ال .. ال
+
+810
+01:04:16,610 --> 01:04:20,790
+top تبعها مختلف فبيكون .. كل لون بيعطي .. بي ..
+
+811
+01:04:20,790 --> 01:04:24,630
+بيدللي ان ال .. ال .. ال anticoagulant اللي في
+
+812
+01:04:24,630 --> 01:04:30,250
+الداخل مختلف تمام؟ يعني هاي ال .. ال .. ال tube
+
+813
+01:04:30,250 --> 01:04:34,550
+اللي ال top تبعها red طبعا هذا يا بناتي يعني شيء
+
+814
+01:04:34,550 --> 01:04:38,530
+معمم، ما .. يعني ما ينفعش .. يعني مش مطبق في دولة
+
+815
+01:04:38,530 --> 01:04:43,190
+أو دولة لأ، لأ هي كل الأنابيب هيك بتكون، تمام؟
+
+816
+01:04:43,190 --> 01:04:47,330
+فبالتالي الأنابيب اللي ال red .. ال .. ال top تبعها
+
+817
+01:04:47,330 --> 01:04:50,510
+لونه red حكينا ايش بسميها plain tube يعني مافيش
+
+818
+01:04:50,510 --> 01:04:57,410
+فيها أي حاجة فاضية no أو none additive تمام؟ فهذه
+
+819
+01:04:57,410 --> 01:05:01,390
+ال non additive ايش يعني؟ يعني مافيش فيها أي
+
+820
+01:05:01,390 --> 01:05:07,210
+اضافات فالدم هيفصل ويتجلط ولما أفصل هيعطيني
+
+821
+01:05:07,210 --> 01:05:11,490
+serum تمام؟ في عندي ال .. ال top اللي لونه orange
+
+822
+01:05:11,490 --> 01:05:18,140
+في .. خلينا نشوف .. نرجع للصورة هنا اللي لونه هيك
+
+823
+01:05:18,140 --> 01:05:22,980
+على orange أو على أصفر فيها clot activator تمام؟ و
+
+824
+01:05:22,980 --> 01:05:27,680
+هذا ليه .. ليه clot activator؟ يعني منشط للتجلط
+
+825
+01:05:27,680 --> 01:05:32,660
+فبرضه ال blood هيتجلط فيه وهيعطيني serum وايضا
+
+826
+01:05:32,660 --> 01:05:36,980
+نفس الشيء اللي .. اللي لونه yellow اللي لونه أصفر
+
+827
+01:05:36,980 --> 01:05:42,300
+صريح برضه كمان فيه clot activator وفيه gel فهدول
+
+828
+01:05:42,300 --> 01:05:47,020
+برضه كمان بينشطوا عملية التجلط فال blood هيتجلط و
+
+829
+01:05:47,020 --> 01:05:51,460
+لما أفصله هيعطيني serum تمام؟ ال heparin tube
+
+830
+01:05:51,460 --> 01:05:55,260
+بيكون لونها green في هذا اللون، لون ال mint green
+
+831
+01:05:55,260 --> 01:05:58,960
+اللي هو الأخضر اللي .. اللي ماخد اللون النعناعي
+
+832
+01:05:58,960 --> 01:06:05,100
+تمام؟ heparin tube فهذا ال .. ال tube فيه heparin
+
+833
+01:06:05,100 --> 01:06:08,320
+اذا الدم مش هيتجلط فاذا لما أفصله بيعطيني plasma
+
+834
+01:06:08,930 --> 01:06:12,930
+تمام؟ اما heparin sodium أو heparin lithium بيكون
+
+835
+01:06:12,930 --> 01:06:16,970
+فيه .. تمام؟ اللي لونه light green الأخضر الفاتح
+
+836
+01:06:16,970 --> 01:06:21,410
+بيكون موجود فيه gel و heparin lithium تمام؟ وبي
+
+837
+01:06:21,410 --> 01:06:25,590
+.. و .. و بيفصل ال .. طبعا .. طبعا الدم مش هيتجلط
+
+838
+01:06:25,590 --> 01:06:29,030
+مدام فيه heparin فبالتالي لما أفصله بيعطيني plasma
+
+839
+01:06:29,030 --> 01:06:33,490
+ايش يعني plasma؟ يعني ان السائل ما .. ما .. ما تم
+
+840
+01:06:33,490 --> 01:06:37,250
+استهلاك العوامل التجلط اللي موجودة في
+
+889
+01:10:01,220 --> 01:10:04,740
+أو على .. على جدار الـtube إما على الجانب أو حتى
+
+890
+01:10:04,740 --> 01:10:08,160
+في .. يعني هذا أحيانا بيكون هو نفسه علامة فلون في
+
+891
+01:10:08,160 --> 01:10:12,780
+علامة عندي، فلون بعبي أنا blood فبالتالي بيحدد لي
+
+892
+01:10:12,780 --> 01:10:16,000
+الـvolume، طبعا، في tubes بتكون مصممة لـvolume
+
+893
+01:10:16,000 --> 01:10:20,700
+قليل، في tubes بتكون مصممة لـvolume أكثر حسب نوع
+
+894
+01:10:20,700 --> 01:10:26,220
+الفحص أو الفحوصات اللي مطلوبة، يعني إذا أنا بدي ..
+
+895
+01:10:26,220 --> 01:10:29,400
+بدي أشتغل من الـtubes هذي عدة فحوصات، بختار الـ
+
+896
+01:10:29,400 --> 01:10:32,740
+tubes اللي حجم .. بتعطيني حجم أكثر، أما إذا كانت
+
+897
+01:10:32,740 --> 01:10:36,060
+الـ .. الفحوصات قليلة فبختار الـtubes اللي بت
+
+898
+01:10:36,060 --> 01:10:40,760
+.. بتعبأ أو بيكون فيها أو بتعطيني حجم أقل هذا
+
+899
+01:10:40,760 --> 01:10:45,660
+بالنسبة لمحاضرتنا لليوم بنكون هيك خلصنا تعرفنا على
+
+900
+01:10:45,660 --> 01:10:50,420
+مكونات الدم و الـanticoagulant و الأنواع الأكثر
+
+901
+01:10:50,420 --> 01:10:55,780
+common و وظائفها، تمام؟ عندكم أي سؤال، أي استفسار
+
+902
+01:10:55,780 --> 01:11:00,660
+بعتوه لي بقى على group الـwhatsapp بجاوبكم من خلال
+
+903
+01:11:00,660 --> 01:11:05,700
+تسجيل صوتي أو بنتناقش فيه عبر محاضرة الـzoom اللي
+
+904
+01:11:05,700 --> 01:11:10,440
+موعدها يوم السبت، يعطيكم العافية و موفقين بإذن الله
diff --git a/PL9fwy3NUQKwY9jl8xw-bWkVZkWcGGyspd/mJoJF0VELyo_postprocess.srt b/PL9fwy3NUQKwY9jl8xw-bWkVZkWcGGyspd/mJoJF0VELyo_postprocess.srt
new file mode 100644
index 0000000000000000000000000000000000000000..794ba8251d8ff6c2b37a5771fdf1e014da5e92f2
--- /dev/null
+++ b/PL9fwy3NUQKwY9jl8xw-bWkVZkWcGGyspd/mJoJF0VELyo_postprocess.srt
@@ -0,0 +1,3616 @@
+1
+00:00:04,390 --> 00:00:07,310
+السلام عليكم ورحمة الله وبركاته اليوم ان شاء الله
+
+2
+00:00:07,310 --> 00:00:11,690
+رح نبدأ محاضرة جديدة بعنوان blood components
+
+3
+00:00:11,690 --> 00:00:16,710
+مكونات الدم بالبداية ما هو تعريف الدم؟ ما هو ال
+
+4
+00:00:16,710 --> 00:00:22,530
+blood؟ تمام ال blood أو الدم هو عبارة عن السائل
+
+5
+00:00:22,530 --> 00:00:27,990
+الأحمر the red fluid that oozes out of your body
+
+6
+00:00:27,990 --> 00:00:31,690
+when you have sustained a cut or deep injuryإذا
+
+7
+00:00:31,690 --> 00:00:40,210
+كان في جرح أو يعني خدش لل skin أو للأوعية الدموية
+
+8
+00:00:40,210 --> 00:00:46,690
+تمام بحيث أنه هذا الجرح أخذ وقت استغرق وقت قبل
+
+9
+00:00:46,690 --> 00:00:52,490
+التقامه فأكيد رح يخرج منه سائل أحمر هو ال blood
+
+10
+00:00:52,490 --> 00:00:56,860
+تمام و طبعا احنا اكيد يعني we are familiarwith
+
+11
+00:00:56,860 --> 00:01:00,480
+blood احنا بنعرف ال blood وشوفنا ال blood قبل هيك
+
+12
+00:01:00,480 --> 00:01:09,120
+و بنعرف يعني إيش مكوناته، تمام؟ طيب فالسائل الأحمر
+
+13
+00:01:09,120 --> 00:01:15,310
+هذا هو عبارة عن سائل أكتركتر كثافة من الماء و ايضا
+
+14
+00:01:15,310 --> 00:01:20,470
+تقريبا من تلت لاربع مرات اكتر viscosity اكتر لزوجة
+
+15
+00:01:20,470 --> 00:01:26,490
+من الماء ايضا blood volume حجم السائق حجم السائل
+
+16
+00:01:26,490 --> 00:01:31,450
+هذا الأحمر او الدم طبعا هو متغير variable يختلف من
+
+17
+00:01:31,450 --> 00:01:36,970
+شخص الى اخر ولكن هو غالبا بيكون تمانية في المية من
+
+18
+00:01:36,970 --> 00:01:42,110
+كتلة جسم الإنسان يعني احنا طبعا كتلة جسمنامتغيرة،
+
+19
+00:01:42,110 --> 00:01:47,110
+في ناس بيكونوا اطوال اسمان، في ناس بيكونوا نحاف،
+
+20
+00:01:47,110 --> 00:01:51,190
+اقصار، فكتلة جسمهم متغيرة، فبالتالي حجم الدم
+
+21
+00:01:51,190 --> 00:01:56,710
+متغير، ولكن المتعارف عليه ان هو بيحتل تمانية في
+
+22
+00:01:56,710 --> 00:01:59,590
+المية أو يعني بيكون .. بياخد تمانية في المية من
+
+23
+00:01:59,590 --> 00:02:04,870
+كتلة الجسممثلا لو كان شخص وزنه خمسين kilogram
+
+24
+00:02:04,870 --> 00:02:09,050
+معناها point zero تمانية في المية يعني تمانية من
+
+25
+00:02:09,050 --> 00:02:14,570
+مية، تمام؟ ضرب الخمسين بيكون حجم ال blood تبعه
+
+26
+00:02:14,570 --> 00:02:18,870
+أربعة liter الناس اللي .. اللي وزنها أكتر من خمسين
+
+27
+00:02:18,870 --> 00:02:22,330
+هيكون عندها blood volume أكتر و الناس اللي وزنها
+
+28
+00:02:22,330 --> 00:02:26,650
+أقل من خمسين أكيد رح يكون عندهم blood volume أقل،
+
+29
+00:02:26,650 --> 00:02:30,880
+تمام؟فإذا أعطيناكم وزن بالامتحان و حكيتلكم إنه
+
+30
+00:02:30,880 --> 00:02:34,520
+احسي بيه لقداش بيكون عنده blood volume بس ماعليك
+
+31
+00:02:34,520 --> 00:02:39,600
+إلا إنك أنت تدر ب 8 من 100 أو 8 على 100 في الوزن
+
+32
+00:02:39,600 --> 00:02:46,260
+تمام؟ طيب هناك عوامل أخرى تؤثر على الحجم الدم أو
+
+33
+00:02:46,260 --> 00:02:49,560
+ال blood volume ألا وهي ال body size احنا حكينا
+
+34
+00:02:49,560 --> 00:02:53,930
+يعني ال ..كتلة الجسم حجم الجسم بيأثر على حجم ال
+
+35
+00:02:53,930 --> 00:02:59,150
+blood amount of adipocyte كمان نسبة النسيج الدهني
+
+36
+00:02:59,150 --> 00:03:03,430
+في الجسم برضه كمان بتأثر على حجم ال blood و ال
+
+37
+00:03:03,430 --> 00:03:08,490
+electrolyte concentration جداش تركيز الأملح
+
+38
+00:03:08,490 --> 00:03:14,440
+والمعادن داخل الجسم أيضا بتأثر على حجم الدمولكن ال
+
+39
+00:03:14,440 --> 00:03:19,300
+.. ال .. الحجم اللي قدام يتراوح ما بين 4 لتر إلى 6
+
+40
+00:03:19,300 --> 00:03:23,480
+لتر و ال average volume هو 5 لتر يعني معظم الناس
+
+41
+00:03:23,480 --> 00:03:27,520
+بنلاحظ أنها هي في نص ال range واقعة من 4 على 6
+
+42
+00:03:27,520 --> 00:03:34,220
+المنتصف هو 5 لترطيب لو أنا جيت سحبت عينة دم من
+
+43
+00:03:34,220 --> 00:03:40,420
+وريد تمام وحفظيتها في انبوبة و هذه الانبوبة حطيتها
+
+44
+00:03:40,420 --> 00:03:46,140
+في جهاز اسمه centrifuge تمام جهاز الطرد المركزي
+
+45
+00:03:46,140 --> 00:03:52,440
+centrifuge تمام هذا الجهازوظيفته ان هو يفصل
+
+46
+00:03:52,440 --> 00:03:58,520
+لمكونات الدم يفصل مكونات الدم طبعا كيف بيفصلها؟ من
+
+47
+00:03:58,520 --> 00:04:04,300
+اسمه طرد مركزي يعني الأشياء الثقيلة او ال ..
+
+48
+00:04:04,300 --> 00:04:09,840
+الوزنها ثقيل بتنزل لتحت في قاع ال tube و الأشياء
+
+49
+00:04:09,840 --> 00:04:13,920
+الخفيفة او ال .. او ال .. المكونات اللي وزنها خفيف
+
+50
+00:04:13,920 --> 00:04:18,440
+بتكون موجودة في the top of the tube في أعلى ال
+
+51
+00:04:18,440 --> 00:04:21,950
+tubeزي هنا مثلا بعد ما أنا أطلعها من جهاز ال
+
+52
+00:04:21,950 --> 00:04:26,250
+centrifuge تمام بلاحظ أنه الضم فصل إلى هذه
+
+53
+00:04:26,250 --> 00:04:30,510
+المكونات جزء الخلايا اللي موجود في قاع ال tube
+
+54
+00:04:30,510 --> 00:04:35,950
+تمام و جزء السائل اللي موجود في at the top of the
+
+55
+00:04:35,950 --> 00:04:40,790
+tube طبعا الخلايا أثقل لذلك كانت موجودة في قاع ال
+
+56
+00:04:40,790 --> 00:04:45,350
+tube أما السائل فهو أخف فعشان هيك كان موجود في the
+
+57
+00:04:45,350 --> 00:04:48,770
+top of the tubeطيب خلينا نشوف إيش السائل إيش مكو
+
+58
+00:04:48,770 --> 00:04:54,270
+.. إيش اسمه السائل هذا المكون اسمه plasma تمام؟ و
+
+59
+00:04:54,270 --> 00:04:57,370
+المكونات
+
+60
+00:04:57,370 --> 00:05:03,070
+الخلايا في عندي طبقة بيضاء خفيفة جدا كخيط رفيع
+
+61
+00:05:03,070 --> 00:05:07,450
+بسميها ال buffy coat اللي بتكون مكونا أو مع ..
+
+62
+00:05:07,450 --> 00:05:12,850
+مكونا من خلاية دم البيضاء ال white bases و الصفائح
+
+63
+00:05:12,850 --> 00:05:20,240
+الدموية البليتلاتطبعا وفي عندى الطبقة حمراء اللون
+
+64
+00:05:20,240 --> 00:05:25,160
+بتكون موجودة في القاع بسميها الاريثروسايت او خلايا
+
+65
+00:05:25,160 --> 00:05:30,880
+دم الحمراء ال RBCs او ال red blood cell طبعا طيبكل
+
+66
+00:05:30,880 --> 00:05:34,760
+من الطبقة البيضاء و الطبقة الحمراء بسميها formed
+
+67
+00:05:34,760 --> 00:05:39,180
+element هذه هي مكونات الدم، هلأ راح نشوف إيش
+
+68
+00:05:39,180 --> 00:05:43,560
+مكوناته اللي .. اللي .. اللي يعني متحدثين عنها في
+
+69
+00:05:43,560 --> 00:05:49,020
+ال slides هلأ الدم إذا أنا فصلته طبعا راح يعطيني
+
+70
+00:05:49,020 --> 00:05:54,860
+three layers، تلات طبقاتبشوفهم لما أنا بعمل إيش
+
+71
+00:05:54,860 --> 00:05:59,180
+centrifugation لل blood لما أنا بدي يكون عندي دم
+
+72
+00:05:59,180 --> 00:06:04,140
+مفصول بال centrifuge بيكون يعني مفصول إلى تلت
+
+73
+00:06:04,140 --> 00:06:10,500
+طبقات الطبقة الأولى بسميها straw colored liquid أو
+
+74
+00:06:10,500 --> 00:06:16,220
+بسميها plasma هي سائلماخذ اللون القشي straw
+
+75
+00:06:16,220 --> 00:06:21,200
+colored liquid سائل ماخذ اللون القشي و بسميه ال
+
+76
+00:06:21,200 --> 00:06:26,700
+plasma موجود at the top لإنه خفيف تمام و بيحتل
+
+77
+00:06:26,700 --> 00:06:31,780
+تقريبا خمسة و خمسين في المية من حجم الدم في عندي
+
+78
+00:06:31,780 --> 00:06:37,000
+طبقة بسميها thin cream colored layer يعني طبقة
+
+79
+00:06:37,000 --> 00:06:43,550
+رفيعةو لونها كريمي لونها لون أبيض بسميها ال buffy
+
+80
+00:06:43,550 --> 00:06:49,090
+coat و هذه ال buffy coat هي عبارة عن إيش؟ ال white
+
+81
+00:06:49,090 --> 00:06:54,290
+blood cells خلايا الدم البيضاء and ال platelets
+
+82
+00:06:54,290 --> 00:07:00,430
+الصفائحتمام و هذه طبعا بيكون حجمها أقل من واحد في
+
+83
+00:07:00,430 --> 00:07:04,810
+المية تمام؟ أقل من واحد في المية أو تقريبا يعني
+
+84
+00:07:04,810 --> 00:07:09,250
+احنا بنقربها بنحكي واحد في المية الطبقة التالتة
+
+85
+00:07:09,250 --> 00:07:14,790
+اللي هي at the bottomفي قاع ال tube هي طبقة ال red
+
+86
+00:07:14,790 --> 00:07:20,210
+blood cells وهي عبارة عن heavy layer طبقة ثقيلة
+
+87
+00:07:20,210 --> 00:07:28,270
+وموجودة في البطم في قاع ال tube و تحتل حجم 45% من
+
+88
+00:07:28,270 --> 00:07:32,520
+ال whole blood من عينة الدم اللي أنا سحبتهاطبعا ال
+
+89
+00:07:32,520 --> 00:07:38,140
+cells هذه الخلايا بتكون عبارة عن معلقة في داخل
+
+90
+00:07:38,140 --> 00:07:43,820
+سائل طبعا معلقة داخل سائل فاللي هو ال plasma يعني
+
+91
+00:07:45,120 --> 00:07:48,880
+فإذا أنا ممكن أفصل مكونات الدم إما عن طريق ال
+
+92
+00:07:48,880 --> 00:07:53,300
+filtration باستخدام filter أو باستخدام ال
+
+93
+00:07:53,300 --> 00:07:56,980
+centrifugation اللي هو باستخدام جهاز لل centrifuge
+
+94
+00:07:56,980 --> 00:08:02,220
+أعمله spinning دوران أو التفاف لهذا الجهاز مجرد ما
+
+95
+00:08:02,220 --> 00:08:08,100
+أشغله فيه الكهربا بيصير يعمل دوران التفافات تمام
+
+96
+00:08:08,100 --> 00:08:13,240
+سريعة جدا وبالتالي على إثر هذه التفافات الأجزاء
+
+97
+00:08:13,240 --> 00:08:18,440
+الثقيلة منالسائل بتهبط للأسفل بتنزل at the bottom
+
+98
+00:08:18,440 --> 00:08:22,460
+of the tube و الأجزاء الخفيفة بتكون موجودة at the
+
+99
+00:08:22,460 --> 00:08:27,220
+top of the tube اذا لو انا اعتبرت انه العينة كلها
+
+100
+00:08:27,220 --> 00:08:31,020
+هاي اللي انا عبيتها في ال tube 100% تمام لو
+
+101
+00:08:31,020 --> 00:08:35,990
+اعتبرتها انها كلها 100%فال plasma هتحتل تقريبا
+
+102
+00:08:35,990 --> 00:08:39,310
+خمسة و خمسين في المية ال buffy coat يعني واحد في
+
+103
+00:08:39,310 --> 00:08:43,330
+المية او اقل و ال erythrocyte خمسة و اربعين في
+
+104
+00:08:43,330 --> 00:08:47,370
+المية فبالتالي يعني ان هو هم تقريبا كلهم بيكونوا
+
+105
+00:08:47,370 --> 00:08:51,230
+مية في المية ماشي حكينا ان ال buffy coat لونها
+
+106
+00:08:51,230 --> 00:08:56,660
+creamy و thin layerو مكونة من ال white BCs و
+
+107
+00:08:56,660 --> 00:09:00,740
+البلاتلت ال erythrocyte هي الطبقة الحمراء مكونة من
+
+108
+00:09:00,740 --> 00:09:06,360
+ال red blood cell و ال .. ال plasma هي ال .. ال
+
+109
+00:09:06,360 --> 00:09:10,860
+straw colored liquid السائل اللي لونه قش اللون
+
+110
+00:09:10,860 --> 00:09:14,200
+تمام؟ لونه أصفر طيب ال erythrocyte إيش يعني
+
+111
+00:09:14,200 --> 00:09:18,340
+erythrocyte؟ لو أنا جيت قطعتها erythro لحالها
+
+112
+00:09:18,340 --> 00:09:23,500
+erythro لحالها تمام؟ معناها red colorيعني red
+
+113
+00:09:23,500 --> 00:09:28,400
+color sites sites معناها خلايا، تمام؟ ف erythro
+
+114
+00:09:28,400 --> 00:09:33,120
+red و sites خلايا إذا red cells إذا أنا أحكي
+
+115
+00:09:33,120 --> 00:09:39,560
+erythrocyte أو أحكي red blood cells، تمام؟ أو RBCs
+
+116
+00:09:39,560 --> 00:09:44,520
+هي نفس المعنى، أنا بقصد خلايا، الدم الحمراء طيب
+
+117
+00:09:44,520 --> 00:09:51,970
+نيجي لـ plasma هو أولسائل يعني بيكون هو طبقة من
+
+118
+00:09:51,970 --> 00:09:55,670
+طبقات ال blood و بتكون موجودة at the bottom خلينا
+
+119
+00:09:55,670 --> 00:09:59,030
+نحكي عنها و نشوف إيش هي إيش هي ال plasma من إيش
+
+120
+00:09:59,030 --> 00:10:03,270
+مكوّنة طبعا حكينا إنه هي straw colored liquid و
+
+121
+00:10:03,270 --> 00:10:10,710
+تقريبا تشغل حيث 55% من الدم و طبعا هي عبارة عن ال
+
+122
+00:10:10,710 --> 00:10:16,390
+transport mediumهي الخلايا الدم بتكون معلقة في هذه
+
+123
+00:10:16,390 --> 00:10:20,770
+ال plasma إذا هي بتتكفل في نقل خلايا الدم من و إلى
+
+124
+00:10:20,770 --> 00:10:25,650
+الأعضاء الجسم، تمام؟ إذا بتنقل ل blood cells و
+
+125
+00:10:25,650 --> 00:10:31,970
+لplatelet من و إلى أعضاء جسم الإنسان، تمام؟ هذه هي
+
+126
+00:10:31,970 --> 00:10:37,690
+السائلوطبعا لما انا احكي ان ال plasma تحتل نسبة 55
+
+127
+00:10:37,690 --> 00:10:46,330
+% من الدم في العينة او 55% من الدم في الجسم فانا
+
+128
+00:10:46,330 --> 00:10:50,590
+المعلومة واحدة يعني لإن انا بما اني أخدت عينة من
+
+129
+00:10:50,590 --> 00:10:55,490
+الدمو كانت هذه العينة ممثلة أو معبرة عن الدم فلما
+
+130
+00:10:55,490 --> 00:11:00,710
+أجي أفصلها تمام راح أفصل نسبة البلازمة بالنسبة
+
+131
+00:11:00,710 --> 00:11:04,350
+لهذه العينة هي نفسها نسبة البلازمة بالنسبة للدم
+
+132
+00:11:04,350 --> 00:11:09,790
+كله اللي موجود داخل جسم الإنسان تمام؟ طيبلذا لما
+
+133
+00:11:09,790 --> 00:11:15,130
+أحكي 55% من ال blood أو من ال سان بالنفس المعنى
+
+134
+00:11:15,130 --> 00:11:20,810
+طيب و مكونات هاي ال plasma من إيش مكونات؟ احنا
+
+135
+00:11:20,810 --> 00:11:25,270
+حكينا إن هي سائل أصفر اللون ولكن ما هي مكوناته؟
+
+136
+00:11:25,270 --> 00:11:30,290
+أكثر من 90% من هذا السائل هو عبارة عن water just
+
+137
+00:11:30,290 --> 00:11:35,780
+إيش water ال plasma هيماء بالأساس، تمام؟ أكتر من
+
+138
+00:11:35,780 --> 00:11:43,360
+90% منه ماء ولكن الأقل من 10% اللي ضايقين هي عبارة
+
+139
+00:11:43,360 --> 00:11:50,920
+عن أيوناتأملاح تمام ايونات اكساديوم كلورايد و
+
+140
+00:11:50,920 --> 00:11:55,400
+بتاسيوم اي اشي .. اي ايونات او املاح موجودة داخل
+
+141
+00:11:55,400 --> 00:12:00,180
+الجسم بروتينات اللي زي ال albumin و ال globulin و
+
+142
+00:12:00,180 --> 00:12:05,900
+ال fibrinogen بروتينات اللي بتعمل تخ .. تخثر للدم
+
+143
+00:12:05,900 --> 00:12:09,940
+موجودة ايضا في البلازما الانزيمات اللي بتقوم
+
+144
+00:12:09,940 --> 00:12:13,980
+بالوظائف المختلفة موجودة داخل البلازما هي عبارة عن
+
+145
+00:12:13,980 --> 00:12:19,750
+بلازما بروتينو أيضًا dissolved gases أو dissolved
+
+146
+00:12:19,750 --> 00:12:25,930
+gases، تمام؟ غازات مذابة داخل الدم بتكون موجودة في
+
+147
+00:12:25,930 --> 00:12:29,730
+البلازمة زي ال oxygen و يتعني أكسيد الكربون و
+
+148
+00:12:29,730 --> 00:12:34,930
+أيضًا موجود عباء .. موجود ال nutrients يعني مغذيات
+
+149
+00:12:34,930 --> 00:12:39,890
+أو مصادر للطاقة زي ال glucose و الأحماض الأمينية و
+
+150
+00:12:39,890 --> 00:12:43,030
+الأحماض الدهنية هذه بتكون موجودة أيضًا داخل
+
+151
+00:12:43,030 --> 00:12:47,170
+البلازمةبالإضافة إلى ال circulating tissue
+
+152
+00:12:47,170 --> 00:12:50,850
+products نواتج اللي بينتجها أو اللي بيطلعها
+
+153
+00:12:50,850 --> 00:12:54,790
+الأعضاء يعني ال west طبع الأعضاء زي اليوريا
+
+154
+00:12:54,790 --> 00:12:58,330
+كرياتينين لكتات هذه كلها ناتجة من ال metabolism
+
+155
+00:12:58,330 --> 00:13:02,830
+للأعضاء أو من الوثائق لإن الأعضاء عملت function في
+
+156
+00:13:02,830 --> 00:13:07,250
+جسم الإنسانفكان ناتج ال .. ال function هذه أو ناتج
+
+157
+00:13:07,250 --> 00:13:12,670
+ال metabolism تبعها ب .. مركبات بدنا نتخلص منها،
+
+158
+00:13:12,670 --> 00:13:16,310
+بدها تطلع إما في ال urine أو في ال stool فبالتالي
+
+159
+00:13:16,310 --> 00:13:20,550
+بنتكون .. إلا تمر عبر الدمتمام؟ اذا ال tissue
+
+160
+00:13:20,550 --> 00:13:24,630
+products او ال tissue waste نفس الاشي زي اليوريا و
+
+161
+00:13:24,630 --> 00:13:28,850
+الكرياتينين و اللكتات circulating hormones ايضا
+
+162
+00:13:28,850 --> 00:13:33,590
+موجود في هذه البلازما هرمونات زي الانسولين و ال
+
+163
+00:13:33,590 --> 00:13:37,850
+adrenaline و ال growth hormone و الهرمونات اخرى
+
+164
+00:13:37,850 --> 00:13:43,210
+تمام؟ موجودة بتكون داخل البلازما اذا البلازما هي
+
+165
+00:13:43,210 --> 00:13:48,360
+عبارة عن السائل الأصفر اللون او القش اللوناللي
+
+166
+00:13:48,360 --> 00:13:53,600
+موجود في at the top of centrifuged blood تمام؟ و
+
+167
+00:13:53,600 --> 00:14:00,720
+بيشكل خمسة و خمسين في المية من حجم الدم مكوناته
+
+168
+00:14:00,720 --> 00:14:06,400
+water، ماء، تمام؟ solvent، ماء و solutes هذه كلها
+
+169
+00:14:06,400 --> 00:14:10,520
+solutes بسميها خلينا نوظف التعريفات اللي احنا
+
+170
+00:14:10,520 --> 00:14:15,360
+اتعلمناها المحاضرة الماضية أي سائل مكون من solutes
+
+171
+00:14:15,360 --> 00:14:20,340
+and solvent صح؟ تمامفالبلازمة هي عبارة عن solvent
+
+172
+00:14:20,340 --> 00:14:25,140
+اللي هو المذيب الماء أما الأشياء المذابة داخله
+
+173
+00:14:25,140 --> 00:14:29,180
+ممكن تكون أيونات، أملاح، بروتينات، غازات،
+
+174
+00:14:29,180 --> 00:14:33,080
+nutrients، موغذيات، tissue product أو تشيوست أو
+
+175
+00:14:33,080 --> 00:14:39,340
+حتى hormones بتكون موجودة طيبفبالتالي إذا ال water
+
+176
+00:14:39,340 --> 00:14:44,040
+هو عبارة عن important .. important solvent مذيب
+
+177
+00:14:44,040 --> 00:14:48,840
+مهم للايونات و ال .. ال solutes اللي بتكون موجودة
+
+178
+00:14:48,840 --> 00:14:52,280
+فيه، ال vitaminات، ال enzymes، ال hormones، ال
+
+179
+00:14:52,280 --> 00:14:55,720
+protein، ال waste، كلها بتكون مضعبة داخل هذه
+
+180
+00:14:55,720 --> 00:15:01,330
+البلازمةطيب، في مصطلح أخر احنا ب .. ب .. ب ..
+
+181
+00:15:01,330 --> 00:15:05,210
+هيتردد معناه و هنستخدمه كتير اللي هو ال serum،
+
+182
+00:15:05,210 --> 00:15:09,090
+تمام؟ ال serum هو عبارة عن إيش؟ هلأ لو أنا ال
+
+183
+00:15:09,090 --> 00:15:15,590
+blood هذا اللي فصلته غير متجلة، تمام؟ إذا كان غير
+
+184
+00:15:15,590 --> 00:15:20,360
+متجلةيعني عوامل التجلط أو بروتينات التجلط ما تم
+
+185
+00:15:20,360 --> 00:15:25,300
+استهلاكها فمعناها إن هذا السائل اللي .. اللي ..
+
+186
+00:15:25,300 --> 00:15:31,480
+اللي موجود في الطب بسميه plasma، تمام؟ بسميه
+
+187
+00:15:31,480 --> 00:15:36,370
+plasmaلكن إذا كان الدم متجلط، إيش يعني الدم متجلط؟
+
+188
+00:15:36,370 --> 00:15:40,170
+يعني أصبح عبارة عن كتلة، بطل سائل، خلايا الدم
+
+189
+00:15:40,170 --> 00:15:45,110
+تجمعت و الـplatelet و الصفائح الدموية تجمعت و أصبح
+
+190
+00:15:45,110 --> 00:15:48,910
+الدم عبارة عن كتلة أو إشي صلب، سُلِد، تمام؟ بطل
+
+191
+00:15:48,910 --> 00:15:55,690
+سائل، معناه السائل اللي فوق بينفصل، بسمي serum،
+
+192
+00:15:55,690 --> 00:16:00,380
+تمام؟ طيبفي حالة البلازمة بت .. ال .. ال .. طبعا
+
+193
+00:16:00,380 --> 00:16:05,020
+ال .. الدم يا بنات موجود فيه بروتينات بسميها عوامل
+
+194
+00:16:05,020 --> 00:16:10,400
+تجلط، هذه البروتينات بتتنشط لما الدم يتجلط و
+
+195
+00:16:10,400 --> 00:16:15,740
+بتستهلك، تمام؟فعشان هيك إذا كان الدم غير متجلط
+
+196
+00:16:15,740 --> 00:16:19,440
+بتكون بروتينات التخثر أو عوامل التخثر أو عوامل
+
+197
+00:16:19,440 --> 00:16:23,440
+التجلط غير مستهلكة يعني لساتها موجودة لإن الدم ما
+
+198
+00:16:23,440 --> 00:16:28,460
+اتجلط فبسميش السائل اللي فوق plasma يعني بيحتوي
+
+199
+00:16:28,460 --> 00:16:33,360
+على fibrinogen تمام؟بيحتوي على fibrinogen لإنه مش
+
+200
+00:16:33,360 --> 00:16:37,000
+مستهلك ال .. ال .. ال fibrinogen ما تم استهلاكه
+
+201
+00:16:37,000 --> 00:16:43,340
+طيب فإذا ال plasma هي عبارة عن سائل الموجود at the
+
+202
+00:16:43,340 --> 00:16:48,700
+top of قاش الدم اللي لساته ما اتجلطتمام؟ أو اللي
+
+203
+00:16:48,700 --> 00:16:53,660
+غير متجلط وموجود فيه fibrinogen أما ال serum فهو
+
+204
+00:16:53,660 --> 00:16:58,120
+السائل اللي موجود at the top of ash clotted blood
+
+205
+00:16:58,120 --> 00:17:04,480
+على سطح الدم المتجلط وطالما أن الدم اتجلط معناها
+
+206
+00:17:04,480 --> 00:17:08,940
+تم استهلاك عوامل التخثر تم استهلاك بروتينات التخثر
+
+207
+00:17:08,940 --> 00:17:13,580
+فبالتالي السائل اللي موجود at the top لا يحتوي على
+
+208
+00:17:13,580 --> 00:17:20,490
+fibrinogen فبالتالي ashهو عبارة عن serum طبعا؟ إذا
+
+209
+00:17:20,490 --> 00:17:26,030
+لو أنا حكيت plasma ناقص fibrinogen إيش هيكون؟
+
+210
+00:17:26,030 --> 00:17:31,150
+هيكون serum طبعا؟ plasma ناقص fibrinogen هيكون
+
+211
+00:17:31,150 --> 00:17:36,230
+عبارة عن serum لإنه حكينا إنه ال serum يعني معناها
+
+212
+00:17:36,230 --> 00:17:39,950
+إن الدم اتجلط يعني تم استهلاك البروتينات التخطر
+
+213
+00:17:39,950 --> 00:17:43,450
+يعني تم استهلاك ال fibrinogen يعني مش موجود فيها
+
+214
+00:17:43,450 --> 00:17:47,580
+fibrinogenطبعا، إذا إيش بيفرق البلازمة عن ال ..
+
+215
+00:17:47,580 --> 00:17:50,980
+السيرام؟ هي فقط في مك .. في .. في محتويات عوامل
+
+216
+00:17:50,980 --> 00:17:55,120
+التخثر، البلازمة لساتها عوامل التخثر موجودة و غير
+
+217
+00:17:55,120 --> 00:17:59,200
+مستهلكة، أما السيرام تم استهلاكها، البلازمة فيها
+
+218
+00:17:59,200 --> 00:18:03,800
+فيبرينوجين، السيرام مافيش فيه فيبرينوجين، هذا هو
+
+219
+00:18:03,800 --> 00:18:08,840
+الفرق ما بينهمتمام؟ إذا نيجي نشوف ال serum هو
+
+220
+00:18:08,840 --> 00:18:12,560
+عبارة إن ال plasma differs slightly from what is
+
+221
+00:18:12,560 --> 00:18:17,780
+termed serum ال plasma بتختلف قليلا عن ما يسمى بال
+
+222
+00:18:17,780 --> 00:18:23,210
+serum إذا الإختلاف بسيط فيهمالسيرام هو عبارة عن
+
+223
+00:18:23,210 --> 00:18:27,850
+إيش؟ generally taken to mean the fluid part of the
+
+224
+00:18:27,850 --> 00:18:31,670
+blood which remain after the blood has coagulated
+
+225
+00:18:31,670 --> 00:18:37,090
+أو clotted هو عبارة عن ال fluid السائل اللي يتبقى
+
+226
+00:18:37,090 --> 00:18:41,830
+بعد إيش أو ينفصل at the top بعد إيش after the
+
+227
+00:18:41,830 --> 00:18:46,290
+blood has coagulated يعني clottedبعد ما الدم يصير
+
+228
+00:18:46,290 --> 00:18:50,850
+له coagulation يصير له clotted فبالتالي وينفصله
+
+229
+00:18:50,850 --> 00:18:55,690
+فالسائل الموجود at the top بسميه serum وإيش
+
+230
+00:18:55,690 --> 00:18:59,390
+معناها؟ مدام صار الدم .. لو الدم صار متخفر صار
+
+231
+00:18:59,390 --> 00:19:05,840
+متجلط تمام تحت في at the bottomمعناها تم استهلاك
+
+232
+00:19:05,840 --> 00:19:11,280
+بروتينات التخطر معناها مافيش فيه fibrinogen هذا
+
+233
+00:19:11,280 --> 00:19:15,480
+السائل القش اللون فبقى اسمه plasma وصار اسمه serum
+
+234
+00:19:18,070 --> 00:19:24,690
+طيب المكون الثاني من مكونات الدم هي ال red blood
+
+235
+00:19:24,690 --> 00:19:28,890
+cell تمام؟ هي الطبقة الحمراء اللي موجودة at the
+
+236
+00:19:28,890 --> 00:19:32,590
+bottom red blood cell هلأ ال red blood cell يا
+
+237
+00:19:32,590 --> 00:19:36,770
+بنات لو أنا جيت خلاية دم الحمراء ال erythrocyte
+
+238
+00:19:36,770 --> 00:19:43,320
+يعني تمام؟ لو أنا جيتطلعت عليها نظرة من .. يعني لو
+
+239
+00:19:43,320 --> 00:19:46,620
+أنا حطيتها تحت ال microscope و طلعت عليها من ال ..
+
+240
+00:19:46,620 --> 00:19:50,440
+من أعلى بشوفها عبارة عن discotheque قرصية زي شكل
+
+241
+00:19:50,440 --> 00:19:57,340
+ال CD مدورة تمام؟ و إذا كان أنا جيت و طلعت عليها
+
+242
+00:19:57,340 --> 00:20:02,280
+من الجانب من ال side view نظرة جانبية من جنبها
+
+243
+00:20:02,280 --> 00:20:06,860
+بشوفها بايكنكافشاب تمام؟ زي الساعة الرملية بيكون
+
+244
+00:20:06,860 --> 00:20:15,540
+شكلها بايكنكافشاب تمام؟طيب و تشغل 99% من خلايا
+
+245
+00:20:15,540 --> 00:20:21,220
+الدم لإنه عددها بالملايين خلايا ال RBCs طبعا أهم
+
+246
+00:20:21,220 --> 00:20:28,100
+إشي في ال RBCs أو أهم وظيفة لل RBCs إنها تحمل ال
+
+247
+00:20:28,100 --> 00:20:31,520
+red colored hemoglobin البروتين اللي اسمه
+
+248
+00:20:31,520 --> 00:20:36,860
+hemoglobin اللي لونه أحمر تمام؟عشان هيك هي اكتسبت
+
+249
+00:20:36,860 --> 00:20:41,480
+اللون الأحمر نسبة لوجود فيها .. لوجود أو لاحتواءها
+
+250
+00:20:41,480 --> 00:20:45,360
+على البروتين الأحمر في داخلها، طبعا؟ فعشان هيك
+
+251
+00:20:45,360 --> 00:20:49,940
+اسمها أخدت لونها أو .. واسمها من لون و البروتين
+
+252
+00:20:49,940 --> 00:20:56,120
+اللي موجود في داخلها و اللي بيشغل تقريبا 70% من
+
+253
+00:20:56,120 --> 00:21:02,170
+حجمهاطيب؟ و ال .. هذا ال homoglobin هو عبارة عن ال
+
+254
+00:21:02,170 --> 00:21:10,130
+protein موجود في حلقات iron أو iron .. يعني هو
+
+255
+00:21:10,130 --> 00:21:13,250
+عبارة عن iron containing ال protein، موجود فيه
+
+256
+00:21:13,250 --> 00:21:19,530
+حديد، تمام؟و هذا السبب لإن لونه أحمر وجود ذرات
+
+257
+00:21:19,530 --> 00:21:25,570
+الحديد فيه هي اللي بتكسبه اللون الأحمر طبعا هو هذا
+
+258
+00:21:25,570 --> 00:21:30,290
+البروتين بيحمل تقريبا 97% من ال oxygen اللي موجود
+
+259
+00:21:30,290 --> 00:21:34,980
+في جسمناتلاتين في المية .. sorry تلاتة .. تلاتة في
+
+260
+00:21:34,980 --> 00:21:39,440
+المية من ال oxygen اللي موجود في جسمنا موجودة على
+
+261
+00:21:39,440 --> 00:21:42,960
+بروتينات ال myoglobin اللي موجودة في العضلات و ال
+
+262
+00:21:42,960 --> 00:21:49,340
+cytochrome اللي موجودة في .. في ..في الخلايا
+
+263
+00:21:49,340 --> 00:21:54,280
+للتنفس، يعني للـ Oxygen أو للـ Energy Production،
+
+264
+00:21:54,280 --> 00:21:58,820
+لإنتاج الطاقة، هتاخدوا في الحياوية دورة Eclipse،
+
+265
+00:21:58,820 --> 00:22:03,880
+وهتاخدوا الانزيمات اللي اسمة الـ Cytochrome،
+
+266
+00:22:03,880 --> 00:22:08,480
+وهتشوفوا أن هي لازم أنها تعطي Electron للـ Oxygen
+
+267
+00:22:08,480 --> 00:22:12,280
+عشان يصير فيه طاقة، إذن هي تحتوي على Oxygen3% من
+
+268
+00:22:12,280 --> 00:22:16,000
+الأكسجين موجود على الmyoglobin و الcytochrome أما
+
+269
+00:22:16,000 --> 00:22:20,400
+97% من الأكسجين الموجود في جسم نوين موجود محمّذ
+
+270
+00:22:20,400 --> 00:22:24,100
+على ال homoglobin و ال homoglobin هو عبارة عن إيش؟
+
+271
+00:22:24,100 --> 00:22:28,380
+هو عبارة عن protein زائد iron ال iron أكسب ال
+
+272
+00:22:28,380 --> 00:22:32,140
+homoglobin اللون الأحمر عشان هيك الخلايا اكتسبت
+
+273
+00:22:32,140 --> 00:22:36,700
+اللون الأحمر و سرنا نسميها ال red blood cell أو ال
+
+274
+00:22:36,700 --> 00:22:47,630
+erythrocyte أو أحيانا أنا باختصرها بحكيلكمRBCs رد
+
+275
+00:22:47,630 --> 00:22:49,990
+بلد سلز
+
+276
+00:22:56,310 --> 00:23:03,350
+طيب ماشي، إذا الخلايا الدم الحمراء وظيفتها ash حمل
+
+277
+00:23:03,350 --> 00:23:07,310
+ال homoglobin يعني oxygen delivery، هي عبارة عن
+
+278
+00:23:07,310 --> 00:23:12,370
+delivery للأكسجين، بتوصل الأكسجين إلى ash around
+
+279
+00:23:12,370 --> 00:23:18,030
+the body، ده في كل .. في كافة مناطق الجسمو ممكن
+
+280
+00:23:18,030 --> 00:23:23,110
+إنها تحمل ثاني أكسيد الكربون بتحمل أو تعود محملة
+
+281
+00:23:23,110 --> 00:23:26,590
+بثاني أكسيد الكربون من ال .. من أنسجة الجسم و من
+
+282
+00:23:26,590 --> 00:23:29,910
+أعضاء الجسم بكمية قليلة طبعا من ثاني أكسيد الكربون
+
+283
+00:23:29,910 --> 00:23:34,930
+و بتم التخلص منها من الرئاتين عن طريق عملية الزفير
+
+284
+00:23:34,930 --> 00:23:42,250
+تمام؟ و بالتالي ..بس احنا بنتخلص من الكم .. من
+
+285
+00:23:42,250 --> 00:23:45,530
+كمية الغاز اللى ما بدناياها من ثانو اكسيد الكربون
+
+286
+00:23:45,530 --> 00:23:49,110
+عن طريق عملية الزفير جزء من ثانو اكسيد الكربون
+
+287
+00:23:49,110 --> 00:23:53,130
+بيكون موجود فى البلازمة لع .. حتى يكونلى blood
+
+288
+00:23:53,130 --> 00:24:00,210
+buffer تمام؟عهقت كربونات و ال carbonic acid
+
+289
+00:24:00,210 --> 00:24:04,270
+فبالتالي بيكون يعطينا blood buffer و احنا عرفنا
+
+290
+00:24:04,270 --> 00:24:07,590
+إيش يعني blood buffer و عرفنا من إيش مكوناته،
+
+291
+00:24:07,590 --> 00:24:11,930
+قولنا weak acid و ال base طبعته أو ال salt طبعه
+
+292
+00:24:11,930 --> 00:24:18,140
+carbonic acid و carbonateطيب ال white blood cells
+
+293
+00:24:18,140 --> 00:24:23,860
+الطبقة التالتة اللي هي بسميها white blood cells ال
+
+294
+00:24:23,860 --> 00:24:28,200
+white blood طبعا يا بنات لها اسم تاني leukocytes
+
+295
+00:24:28,200 --> 00:24:31,660
+لو جيت كلمة ليكو .. ليكو معناها شفافة أو white
+
+296
+00:24:31,660 --> 00:24:36,760
+تمام وcytes يعني خلايا إذا هي خلايا الدم البيضاء
+
+297
+00:24:37,030 --> 00:24:40,970
+تمام؟ أو خلايا الدم اللي مايلها لون، هاي معناها
+
+298
+00:24:40,970 --> 00:24:47,270
+فبالتالي هي عبارة عن جزء مهم و أساسي من ال blood
+
+299
+00:24:47,270 --> 00:24:51,710
+اللي وظيفته defense against external organism
+
+300
+00:24:51,710 --> 00:24:58,010
+بيدافع أو بيحمي الجسم من الكائنات أو الأجسام
+
+301
+00:24:58,010 --> 00:25:03,670
+الغريبة اللي تعتبر foreign غريبة تعتبر pathogen
+
+302
+00:25:03,670 --> 00:25:09,860
+ممرضة لجسم الإنسانطيب why the blood cells خلاي
+
+303
+00:25:09,860 --> 00:25:13,360
+الدم البيضاء ممكن انا اعطيها لقب ال sanitary
+
+304
+00:25:13,360 --> 00:25:16,480
+engineer؟ اش يعني sanitary engineer؟ يعني مهندس
+
+305
+00:25:16,480 --> 00:25:22,000
+اللي مسئول عن عمليات التنظيف والتوظيف داخل الجسم،
+
+306
+00:25:22,000 --> 00:25:27,490
+cleaning upcleaning up what؟ بنظف إيش؟ بنظف الجسم
+
+307
+00:25:27,490 --> 00:25:34,750
+من خلايا ميتة من بقايا التيشو من الأجسام الغريبة
+
+308
+00:25:34,750 --> 00:25:38,770
+المكونات الغريبة اللي بتدخل جسم الإنسان بيروح
+
+309
+00:25:38,770 --> 00:25:43,430
+بيلتهمها وبيتخلص منها لحتى يحمي جسم الإنسان من ال
+
+310
+00:25:43,430 --> 00:25:48,370
+external organisms أو ال external objects يا إما
+
+311
+00:25:48,370 --> 00:25:53,600
+أجسام غريبة أو كائنات غريبةطبعا؟ طيب، في عندي خمس
+
+312
+00:25:53,600 --> 00:25:57,800
+أنواع من ال .. ال white pieces أو الـ leukocytes،
+
+313
+00:25:57,800 --> 00:26:02,180
+في عندي neutrophil و eosinophil و basophil، طبعا؟
+
+314
+00:26:02,180 --> 00:26:06,760
+و في عندي monocytes و lymphocytes، خمس أنواع طيب،
+
+315
+00:26:06,760 --> 00:26:10,900
+خلينا نشوف إيش هم هدولة خلايا الدم أو إيش أنواعهم،
+
+316
+00:26:10,900 --> 00:26:16,020
+نشوف هناعلى خلايا الدم طبعا خلايا الدم احنا كلنا
+
+317
+00:26:16,020 --> 00:26:20,820
+بنعرف ان كلها يتم إنتاجها من إيش؟ من نخاع العضم من
+
+318
+00:26:20,820 --> 00:26:24,680
+مادة النخاع اللي موجودة في داخل العضام بتنتج اللي
+
+319
+00:26:24,680 --> 00:26:29,380
+موجودة طبعا فيها خلايا stem cell خلايا جدعية و هذه
+
+320
+00:26:29,380 --> 00:26:35,000
+الخلايا بتتميز بتنمو و تتميز و تنقسم و حتى تعطيني
+
+321
+00:26:35,000 --> 00:26:38,480
+رد blood cell خلايا الدم الحمراءحكيتلكم لو انا
+
+322
+00:26:38,480 --> 00:26:43,300
+اطلعت عليها من ايش ال .. ال .. ال top view من ال
+
+323
+00:26:43,300 --> 00:26:47,180
+.. من .. من فوق لت .. يعني من فوق اطلع عليها نظرة
+
+324
+00:26:47,180 --> 00:26:50,480
+علوية بشوف .. بشوفها زي على هيئة ال CD قرصية
+
+325
+00:26:50,480 --> 00:26:55,280
+discotheque أما إذا كانت اطلعت عليها من الجانب
+
+326
+00:26:55,280 --> 00:26:58,780
+بشوفها bicon cave ship زي الساعة الرملية يعني
+
+327
+00:26:58,780 --> 00:27:03,960
+مقاعدة الوجهين تمام؟ أما ال white blood cells في
+
+328
+00:27:03,960 --> 00:27:10,460
+عندي أنواعBesophil و Neutrophil وفيه Monocytes و
+
+329
+00:27:10,460 --> 00:27:14,260
+Lymphocytes طيب هدول ال .. ال Azinophil و ال
+
+330
+00:27:14,260 --> 00:27:20,140
+Besophil و ال Neutrophil بسميها خلايا محببة، خلايا
+
+331
+00:27:20,140 --> 00:27:24,100
+محببة إيش يعني خلايا محببة؟ مش .. مش المحببة يعني
+
+332
+00:27:24,100 --> 00:27:28,820
+إنها هي ال favored يعني المفضلة، لأ خلايا محببة
+
+333
+00:27:28,820 --> 00:27:33,310
+يعني إنه موجود في ال cytoplasm تبعهاحبيبات إذا
+
+334
+00:27:33,310 --> 00:27:37,290
+لاحظته هنا موجود في الـcytoplasm تبعها حبيبات عشان
+
+335
+00:27:37,290 --> 00:27:42,350
+هيك بسميها خلايا محببة أما الخلايا الغير محببة
+
+336
+00:27:42,350 --> 00:27:46,090
+يعني إن الـcytoplasm تبعها مش موجود في حبيبات أو
+
+337
+00:27:46,090 --> 00:27:50,190
+لا يورى فيها حبيبات تحت المايكروسكوب فبسميها
+
+338
+00:27:50,190 --> 00:27:54,870
+monocyte و lymphocyte تمام؟ إذا اللي بتنتهي ب site
+
+339
+00:27:54,870 --> 00:27:59,700
+lymphocyte و monocyte هي خلايا غير محببةأما إذا
+
+340
+00:27:59,700 --> 00:28:03,360
+كانت بتنتهي بكلمة fil, xenofil, bezofil,
+
+341
+00:28:03,440 --> 00:28:09,540
+neutrophil فهي خلايا مش محددة و لا ننسى أيضا انه
+
+342
+00:28:09,540 --> 00:28:16,460
+من هذا النخاع في عندي خلايا بتنقسم و بتعطيني ال
+
+343
+00:28:16,460 --> 00:28:22,010
+platelets الصفائح الدمويةتمام؟ خلينا نشوف هى هنا
+
+344
+00:28:22,010 --> 00:28:27,790
+agranulocyte agranul يعني حبيبات site خلايا يعني
+
+345
+00:28:27,790 --> 00:28:33,690
+خلايا معها حبيبات خلايا محببة agranulocyte فإيش
+
+346
+00:28:33,690 --> 00:28:39,000
+موجود في الcytoplasm تبعها؟ موجود حبيباتتمام؟ و ال
+
+347
+00:28:39,000 --> 00:28:43,860
+a-granulocyte ال a لما بيجي في بداية الكلمة بيجي
+
+348
+00:28:43,860 --> 00:28:49,340
+للنفي تمام؟ a-granulocyte يعني خلايا غير محببة
+
+349
+00:28:49,340 --> 00:28:53,580
+يعني ال cytoplasm تبعها مش موجود في حبيبات فبسميها
+
+350
+00:28:53,580 --> 00:28:59,580
+monocyte و lymphocyteتمام؟ نيجي نشوف هنا، هاي هنا
+
+351
+00:28:59,580 --> 00:29:05,440
+عندنا ال .. الخلايا المحببة ال .. ال neutrophil و
+
+352
+00:29:05,440 --> 00:29:10,640
+ال eosinophil و ال basophil شايفين هم خلايا محببة،
+
+353
+00:29:10,640 --> 00:29:16,720
+هي هنا في شكل أوضح عندى هنا ال neutrophilشوف هاي
+
+354
+00:29:16,720 --> 00:29:21,500
+هاي هاي granulocyte اللي هي ال neutrophil ال a
+
+355
+00:29:21,500 --> 00:29:26,460
+-xenophil و ال basophil أما الخلايا ال a
+
+356
+00:29:26,460 --> 00:29:31,360
+-granulocyte يعني غير محببة هي ال monocyte و ال
+
+357
+00:29:31,360 --> 00:29:34,780
+lymphocyte ال cytoplasm تبعها خالي من الحبيبات
+
+358
+00:29:34,780 --> 00:29:39,020
+طبعا هذه الحبيبات اللي فيها مواديعني تقتل
+
+359
+00:29:39,020 --> 00:29:43,900
+الميكروبات، فبنسميها .. يعني الحبيبات هذه عبارة عن
+
+360
+00:29:43,900 --> 00:29:49,500
+دي فيها يعني العتاد و العدة لخلايا اللي هذه من
+
+361
+00:29:49,500 --> 00:29:56,830
+شأنها إنها داثع عن إيش؟ عن جسم الإنسان، تمام؟طيب
+
+362
+00:29:56,830 --> 00:30:02,250
+لو جيت انا هنا هيعندي عينة blood لو فصلتها راح
+
+363
+00:30:02,250 --> 00:30:05,330
+ألاحظ انه انا في عندي إذا الدم غير متجلة معناها
+
+364
+00:30:05,330 --> 00:30:07,970
+الساقل ده إيش اسمه plasma يعني بيحتوي على
+
+365
+00:30:07,970 --> 00:30:12,090
+fibrinogen ال fibrinogen من إيش مكوّن أو sorry ال
+
+366
+00:30:12,090 --> 00:30:16,790
+plasma هي من إيش مكوّنة مكوّنة من ماء بنسبة أكتر
+
+367
+00:30:16,790 --> 00:30:21,670
+من 90% فيها ماء و الباقي solutes تمام الماء هو ال
+
+368
+00:30:21,670 --> 00:30:26,030
+solvent و الباقي solutes إيش هيgases غازات oxygen
+
+369
+00:30:26,030 --> 00:30:33,190
+تعني أكسيد الكربون كربونات waste تمام الفضلات أو
+
+370
+00:30:33,190 --> 00:30:38,630
+ال products زي الكرياتينين واللاكتات واليوريا
+
+371
+00:30:38,630 --> 00:30:44,890
+nutrient تمام مغذيات وفيتامينات glucose amino
+
+372
+00:30:44,890 --> 00:30:50,960
+acids fatty acids بروتينات زي ال ..بروتينات زي
+
+373
+00:30:50,960 --> 00:30:54,660
+الانزيمات و البروتينات المختلفة و البروتينات
+
+374
+00:30:54,660 --> 00:30:58,200
+الناقلة ال albumin و ال globulin و ال fibrinogen و
+
+375
+00:30:58,200 --> 00:31:02,200
+عوامل التجلط هذه كلها بروتينات تمام؟ و أيضا
+
+376
+00:31:02,200 --> 00:31:06,780
+hormones زي ال growth hormone و ال insulin و ال
+
+377
+00:31:06,780 --> 00:31:09,420
+adrenaline و ال cortisone هذه كلها عبارة عن
+
+378
+00:31:09,420 --> 00:31:13,920
+هرمونات بتكون أيضا موجودةداخل من مكونات البلازما
+
+379
+00:31:13,920 --> 00:31:19,120
+تمام؟ فى عندى هنا الطبقة ال thin layer البيضاء اش
+
+380
+00:31:19,120 --> 00:31:22,960
+بسمى .. من اش مكونة؟ من ال white blood cell و
+
+381
+00:31:22,960 --> 00:31:27,300
+البلاتلة و بسميها puffy coat تمام؟ اللى هو الطبقة
+
+382
+00:31:27,300 --> 00:31:31,540
+ال .. او ال .. ال coat الأبيض تمام؟ ال red blood
+
+383
+00:31:31,540 --> 00:31:34,340
+cell هى الجزء الثقيل اللى موجود at the bottom
+
+384
+00:31:34,340 --> 00:31:38,360
+ويعرفنا اش يعني red blood cell هى خلاية دم الحمراء
+
+385
+00:31:38,360 --> 00:31:42,510
+اللى تنقل ال oxygen وظيفتها oxygendelivery تمام
+
+386
+00:31:42,510 --> 00:31:46,210
+وضيفتها oxygen .. oxygen delivery لو كان هذا الدم
+
+387
+00:31:46,210 --> 00:31:52,370
+متخثر معناه هنا في عندى clot خلايا متجلطة و السائل
+
+388
+00:31:52,370 --> 00:31:57,150
+هذا عبارة عن إيش؟ عبارة عن serum بسميه ببطل اسمه
+
+389
+00:31:57,150 --> 00:32:03,410
+plasma اسمه serumطبعا؟ طيب إذا كان هذا السائل اسمه
+
+390
+00:32:03,410 --> 00:32:07,890
+serum يعني الدم تحته متجلط يعني تم استهلاك
+
+391
+00:32:07,890 --> 00:32:12,490
+البروتينات التجلط عوامل التجلط طبعا؟ إذا مافيش
+
+392
+00:32:12,490 --> 00:32:15,830
+فيبرينوجين الفيبرينوجين استهلكناه في التخثر
+
+393
+00:32:15,830 --> 00:32:21,410
+فبالتالي صار هنا إيش؟ عبارة عن بلازمة ناقص
+
+394
+00:32:21,410 --> 00:32:25,600
+فيبرينوجين عشان هيك بسميه serumنفس المكونات فيه بس
+
+395
+00:32:25,600 --> 00:32:29,300
+اللهم ان عوامل التخثر مش موجودة فيه يعني مافيش فيه
+
+396
+00:32:29,300 --> 00:32:32,180
+fibrinogen مافيش فيه thrombin مافيش فيه البروتينات
+
+397
+00:32:32,180 --> 00:32:36,260
+التانية اللي مسئولة عن التخثر فبالتالي بيصير اسمه
+
+398
+00:32:36,260 --> 00:32:40,420
+serum ال plasma و ال serum يعني بيشبهوا بعض في
+
+399
+00:32:40,420 --> 00:32:46,660
+التركيب في اللون ولكن ال plasma تحتوي على عوامل
+
+400
+00:32:46,660 --> 00:32:50,720
+التخثر اللي ما تم استهلاكها لإن الدم تحتها غير
+
+401
+00:32:50,720 --> 00:32:56,550
+متجلط أما هنا ال serumلا يحتوي على عوامل التخثر،
+
+402
+00:32:56,550 --> 00:33:01,350
+يعني الدم متجلة، لو أنا .. لهنا في هذه الحالة، لو
+
+403
+00:33:01,350 --> 00:33:04,830
+أنا السائل كان plasma و الدم غير متجلة، مجرد ما
+
+404
+00:33:04,830 --> 00:33:09,810
+أعمل mix للتيوب، يعني أعملها .. أحركها، تمام؟
+
+405
+00:33:09,810 --> 00:33:14,570
+للتيوب، بيختلط ال .. ال .. الخلايا بالسائل و بيرجع
+
+406
+00:33:14,570 --> 00:33:19,880
+دم تاني، تمام؟ بيرجعيله نفس قوام الدملكن في حالة
+
+407
+00:33:19,880 --> 00:33:23,560
+ال serum هانا serum و هانا ايش؟ دم متجلط، هانا فيه
+
+408
+00:33:23,560 --> 00:33:29,320
+clot لو أنا خلطتهم في بعض، مستحيل يرجع لدم سائل،
+
+409
+00:33:29,320 --> 00:33:34,220
+تمام؟ أو ياخذ قوام الدم زي ما .. لما جمعته أو لما
+
+410
+00:33:34,220 --> 00:33:38,340
+سحبته من المريض، لأ، مستحيل لإن الدم تخثر، خلاص،
+
+411
+00:33:38,340 --> 00:33:42,780
+اتجلط طيب، هذا هو الفرق ما بين ال plasma و ال
+
+412
+00:33:42,780 --> 00:33:47,400
+serum طيب نيجي نشوف ايش كمان حكيلي عن طبقة ال
+
+413
+00:33:47,400 --> 00:33:53,210
+white piecesحكينا إنه مكوّنة من خمس أنواع من
+
+414
+00:33:53,210 --> 00:33:56,730
+الخلايا اللي هي ال .. ال neutrophil, ozinophil و
+
+415
+00:33:56,730 --> 00:34:00,230
+bezophil و حكينا عنهم هدولة granulocyte, monocyte
+
+416
+00:34:00,230 --> 00:34:03,970
+و lymphocyte هدولة agranulocyte بصير إننا بنعرف
+
+417
+00:34:03,970 --> 00:34:08,670
+إيش يعني granulocyte و إيش يعني agranulocyte و إيش
+
+418
+00:34:08,670 --> 00:34:14,390
+الفرق ما بينهم، كلهمالهم دور في ال defense against
+
+419
+00:34:14,390 --> 00:34:18,070
+external organism ولكن الآلية مختلفة يعني مثلا هذه
+
+420
+00:34:18,070 --> 00:34:23,550
+الخلايا المحببة تحتوي في حبيبتها على انزيمات
+
+421
+00:34:23,550 --> 00:34:28,390
+وجزيئات قاتلة لالميكروبات ال monocyte بتلتهم
+
+422
+00:34:28,390 --> 00:34:32,710
+التهام الميكروب ال lymphocytes بتقتل بآلية أخرى
+
+423
+00:34:32,710 --> 00:34:36,290
+كلهم بيقتلوا الأجسام الغريبة و الكائنات الغريبة
+
+424
+00:34:36,290 --> 00:34:41,180
+اللي بتدخل لجسمنا ولكن الآلية مختلفةطبعا؟ إذا إحنا
+
+425
+00:34:41,180 --> 00:34:48,360
+عندنا جيش مدرب للتعرف على الكائنات الغريبة و هذا
+
+426
+00:34:48,360 --> 00:34:53,860
+الجيش .. الجنود تبعته مسلحة بأسلحة مختلفة .. طبعا؟
+
+427
+00:34:53,860 --> 00:34:58,340
+لنيوتروفيل، لإيزينوفيل، لبيزوفيل، لمونوسايتس و
+
+428
+00:34:58,340 --> 00:35:02,580
+للمفوسايتس كل واحدة منهم عندها سلاح مختلف ..
+
+429
+00:35:02,580 --> 00:35:06,760
+مختلفة عن الأخرى في .. لحتى تقتل الكائنات الغريبة
+
+430
+00:35:06,760 --> 00:35:12,290
+فيهطيب مكوّن آخر من مكوّن ال buffy coat احنا حكينا
+
+431
+00:35:12,290 --> 00:35:15,530
+ال buffy coat فيه white pieces وفيه blatelet
+
+432
+00:35:15,530 --> 00:35:20,970
+البلاتلت هي عبارة عن الصفائح الدموية طبعا هي عبارة
+
+433
+00:35:20,970 --> 00:35:27,190
+عن not true cell مش خلايا حقيقية ولكن هي عبارة عن
+
+434
+00:35:27,190 --> 00:35:32,530
+fragment of cytoplasm from certain bone marrow
+
+435
+00:35:32,530 --> 00:35:39,600
+cells اش يعني؟ خلينا نرجع هنا للصورةنشوف هنا في
+
+436
+00:35:39,600 --> 00:35:46,120
+خلايا موجودة داخل البون مارو اسمها Mega Cario
+
+437
+00:35:46,120 --> 00:35:49,860
+Sites تمام؟ خليني أورجيكم إياها هنا لو أنا بدي
+
+438
+00:35:49,860 --> 00:35:58,720
+أشوف أفتح Mega Cario Sites
+
+439
+00:35:58,720 --> 00:36:04,560
+تمام؟ Mega Cario Sites نعمل
+
+440
+00:36:04,560 --> 00:36:09,260
+searchتمام هى ال .. ال .. ال mega-karyocytes خلينى
+
+441
+00:36:09,260 --> 00:36:14,080
+أشوف ال .. ال .. نتيجة الصور هى خلايا ال mega
+
+442
+00:36:14,080 --> 00:36:17,140
+-karyocytes هى خلايا كبيرة طبعا هى أكبر خلية فى
+
+443
+00:36:17,140 --> 00:36:20,860
+البولمارو شايفينها طبعا mega-karyocytes هى mega
+
+444
+00:36:20,860 --> 00:36:24,860
+-karyocytes طبعا هاد ال mega-karyocytes يا بنات هى
+
+445
+00:36:24,860 --> 00:36:28,860
+هى هنا طبعا موجود هاد ال mega-karyocytesبتروح ..
+
+446
+00:36:28,860 --> 00:36:32,160
+موجودة داخل ال bone marrow بتروح عن طرفي ال bone
+
+447
+00:36:32,160 --> 00:36:35,900
+marrow و بيصير ال cytoplasm تبعها يتفتفت و يعطيني
+
+448
+00:36:35,900 --> 00:36:38,880
+جثيمات صغيرة، هذه الجثيمات الصغيرة بتطلع على الدم
+
+449
+00:36:38,880 --> 00:36:43,220
+من برا و بسميها قبلاتلة، صفائح دموية، طبعا هذا يا
+
+450
+00:36:43,220 --> 00:36:46,950
+بنات أنا ما .. مش ..مش طلباكم فيه يعني بس انا عشان
+
+451
+00:36:46,950 --> 00:36:49,990
+اوضحلكم الفكرة هذا ان شاء الله بتدرسوه في مساقات
+
+452
+00:36:49,990 --> 00:36:54,510
+متقدمة لكن انا زي ما بحكيلكم هذه الخلايا ال mega
+
+453
+00:36:54,510 --> 00:36:58,950
+-karyocyte تمام؟ ال mega-karyocyte هاي موجودة داخل
+
+454
+00:36:58,950 --> 00:37:04,110
+البون مارو تمام؟ و أحد مكونات خلايا البون ماروو
+
+455
+00:37:04,110 --> 00:37:09,510
+هذا ال .. ال mega-karyocyte بتروح على طرف البون و
+
+456
+00:37:09,510 --> 00:37:12,790
+لأول ما تقترب من طرف البون بيصير ال cytoplasm
+
+457
+00:37:12,790 --> 00:37:18,110
+تبعها يتفتفت يعطيني جثيمات segments قطع هذه القطع
+
+458
+00:37:18,110 --> 00:37:21,690
+بسميها بلاتلت الصفائح الدموية هذه القطع بسميها إيش
+
+459
+00:37:21,690 --> 00:37:26,550
+الصفائح الصفائح الدموية إذا الخلية إيش اسمها؟
+
+460
+00:37:26,550 --> 00:37:31,450
+الخلية الأم للصفائح الدموية اسمها mega-karyocyte
+
+461
+00:37:31,710 --> 00:37:36,710
+موجودة وين؟ داخل البونمارو، مش موجودة في الدم، لكن
+
+462
+00:37:36,710 --> 00:37:42,050
+هي الcytoplasm تبعها بيتفتفت داخل البونمارو وبيطلع
+
+463
+00:37:42,050 --> 00:37:46,910
+الفتاة .. الفتاة هادا أو الجثيمات هادى بيطلع لأ
+
+464
+00:37:46,910 --> 00:37:52,490
+الدم وعبارة عن إيش؟ بليتلت صفائح دموية طبعا
+
+465
+00:37:52,490 --> 00:37:55,950
+الصفائح دموية كلنا بنعرف إنها إلها دور وين في
+
+466
+00:37:55,950 --> 00:38:01,820
+التخثر بليapart in the clotting of blood إلها دور
+
+467
+00:38:01,820 --> 00:38:05,820
+في ال clotting في التخثر للدم طب خلينا نشوف إيش
+
+468
+00:38:05,820 --> 00:38:10,920
+دورها في التخثر؟ هلأ هاي القصة هادي story تمام عن
+
+469
+00:38:10,920 --> 00:38:14,840
+ال blood clotting و دور البلازمة و دور البلاتلت في
+
+470
+00:38:14,840 --> 00:38:20,600
+ال clotting تمام؟هلأ لنفرض if a damage occur to a
+
+471
+00:38:20,600 --> 00:38:26,120
+blood vessel او circuit .. فإذا صار في عندى خدش أو
+
+472
+00:38:26,120 --> 00:38:31,140
+damage أو جرح أو cut أو injury لل blood vessel
+
+473
+00:38:31,140 --> 00:38:34,440
+تمام؟ يعني مثلا لو انت جرحت أصبعك ما هذا صار فيه
+
+474
+00:38:34,440 --> 00:38:40,580
+تهتك للأوعيات دموية صح؟ عشان هيك بينزل bloodتمام؟
+
+475
+00:38:40,580 --> 00:38:43,460
+فبالتالي إذا صار في عندي damage لهذا ال blood
+
+476
+00:38:43,460 --> 00:38:49,220
+vessel فبقناها ال circulating platelet الصفائح
+
+477
+00:38:49,220 --> 00:38:53,680
+الدموية اللي بتمشي في ال circulation في الدورة
+
+478
+00:38:53,680 --> 00:38:58,400
+الدموية تمام؟ بتروح على طول على منطقة الجرح
+
+479
+00:38:58,400 --> 00:39:03,780
+بيصيرلها trapped at the injury site بتعلق و بتكون
+
+480
+00:39:03,780 --> 00:39:09,450
+موجودة بتلتصق بال injury site في مكان الجرحطبعا
+
+481
+00:39:09,450 --> 00:39:14,050
+هذه ال blatlet بتتراكم و بتعملي حاجة اسمها blatlet
+
+482
+00:39:14,050 --> 00:39:19,830
+blog طبعا بترتبط مع بعض ارتباط وثيق و بتعطيني جلطة
+
+483
+00:39:19,830 --> 00:39:27,040
+او يعني plot ابتدائية اسمها blatlet blogطبعا طيب
+
+484
+00:39:27,040 --> 00:39:31,380
+هذه الخلائق الصفائح الدموية اللي تراكمت وارتبطت مع
+
+485
+00:39:31,380 --> 00:39:35,360
+بعضها البعض و سكرت مكان الجروح بسميها platelet
+
+486
+00:39:35,360 --> 00:39:40,060
+plug طبعا بتغير شكل ال platelet لما بترتبط على
+
+487
+00:39:40,060 --> 00:39:46,040
+مكان الجروح وبيتغير السطح تبعها فبيصير هذا السطح
+
+488
+00:39:46,040 --> 00:39:52,670
+مناسب لحتىإنه بروتينات التخثر اللي موجودة في ال
+
+489
+00:39:52,670 --> 00:39:57,930
+plasma، في الدم، تمام؟ إنها .. إذا لمست سطح
+
+490
+00:39:57,930 --> 00:40:01,930
+البلاتلت تتنشط، هى بتكون موجودة طبعا بروتينات
+
+491
+00:40:01,930 --> 00:40:07,950
+التخثر بتكون موجودة تسير في الدم خاملة، غير ناشطة،
+
+492
+00:40:07,950 --> 00:40:13,560
+لكنإذا لامست سطح البلاتلت ال plug سطح البلاتلت
+
+493
+00:40:13,560 --> 00:40:19,640
+اللى تراكمت على ال injury site تمام و اتغير شكلها
+
+494
+00:40:19,640 --> 00:40:24,820
+و اتغير مكونات سطحها فهذه البروتينات إذا لامست هذا
+
+495
+00:40:24,820 --> 00:40:30,460
+السطح فبتتحول من خاملة إلى ناشطةطبعا يا بنات أنا
+
+496
+00:40:30,460 --> 00:40:34,180
+كام protein عندي؟ طبعا هي سلسلة من البروتينات مش
+
+497
+00:40:34,180 --> 00:40:37,720
+البروتين ولا اتنين ولا تلاتة مش عامل ولا عاملين
+
+498
+00:40:37,720 --> 00:40:43,680
+ولا تلاتة للتخثر ان عندي عوامل للتخثر ما يقارب من
+
+499
+00:40:43,680 --> 00:40:46,660
+اتناش لاربعتاش هتاخدوهم بالتفاصيل يعني انا مابدي
+
+500
+00:40:46,660 --> 00:40:51,260
+افوتلكم بتفاصيل هتاخدوهم في مساقات لاحقة لكن هي
+
+501
+00:40:51,260 --> 00:40:56,380
+عبارة عن سلسلة من البروتينات تمام؟ وهي البروتينات
+
+502
+00:40:58,030 --> 00:41:03,070
+بتتنشط كسلسلة، يعني مثلا أول حاجة العامل رقم 12
+
+503
+00:41:03,070 --> 00:41:11,710
+بنشط 11، 11 طبعا بنشط 9، 9 بنشط 8، 8 بنشط 7 و
+
+504
+00:41:11,710 --> 00:41:18,290
+هكذا، فإذا بيصير سلسل، فالمحصلة إنه بنصل لعامل رقم
+
+505
+00:41:18,290 --> 00:41:22,050
+2 اللي هو ال thrombinتمام؟ بيكون هو عبارة عن
+
+506
+00:41:22,050 --> 00:41:25,950
+ثرومبين .. ثرومبينوجين بيتنشط و بيتكوّن لثرومبين
+
+507
+00:41:25,950 --> 00:41:29,970
+ال ثرومبين هو النوع النشط منه تمام؟ من العامل رقم
+
+508
+00:41:29,970 --> 00:41:34,590
+اتنين هذا العامل رقم اتنين اللي اسمه ثرومبين بيروح
+
+509
+00:41:34,590 --> 00:41:39,730
+بنشط للعامل رقم واحد اللي هو اسمه فيبرينوجين تمام؟
+
+510
+00:41:39,730 --> 00:41:45,000
+ال فيبرينوجين هذا اشماله عبارة عنRod shape
+
+511
+00:41:45,000 --> 00:41:50,120
+syllable protein هو protein ذائب وشكله شكل الرد
+
+512
+00:41:50,120 --> 00:41:57,020
+شكله شكل العصايا يعني شكل رد تمام وموجود في الدم
+
+513
+00:41:57,020 --> 00:42:03,180
+بشكل syllable ذائب تمام إذا تنشط هذا ال fibrinogen
+
+514
+00:42:03,840 --> 00:42:09,800
+عبر ال .. ال thrombin و اتحول ل fibrin الشكل النشط
+
+515
+00:42:09,800 --> 00:42:16,200
+منه فهذا ال fibrin بيكون insolable تمام؟ و هذا ال
+
+516
+00:42:16,200 --> 00:42:20,270
+.. ال protein بيترسب و بيعمللي شبكةبتمسك
+
+517
+00:42:20,270 --> 00:42:24,090
+البروتينات و بتمسك الخلايا ال RBCs بيصيرلها
+
+518
+00:42:24,090 --> 00:42:28,070
+trapped بتتعلق داخل هذه الشبكة و بيكون ليه؟ اللي
+
+519
+00:42:28,070 --> 00:42:32,070
+هي شكل الجلطة الدموية اللي احنا بنشوفها بالشكل ال
+
+520
+00:42:32,070 --> 00:42:37,750
+solid بالشكل الصلب أصبح تم استهلاك ال fibrinogen
+
+521
+00:42:37,750 --> 00:42:42,910
+فالدم اذا اتجلط تمام في الجلطة طبعاتم استهلاك
+
+522
+00:42:42,910 --> 00:42:47,250
+واتحول ل-fibrin وارتبط مع .. مع البلاك اللي تبلغ و
+
+523
+00:42:47,250 --> 00:42:52,690
+.. فعملي جلطة، تمام؟ فأصبح السائل اللي .. اللي
+
+524
+00:42:52,690 --> 00:42:57,070
+موجود في أصل .. ف .. فوق الدم المتجلط معناه إيش
+
+525
+00:42:57,070 --> 00:43:02,130
+اسمه serum ولا يحتوي على fibrinogen ولا يحتوي على
+
+526
+00:43:02,130 --> 00:43:08,600
+عوامل التخثر الأخرى، تمام؟ هذا هو الفرقطيب فإذا ال
+
+527
+00:43:08,600 --> 00:43:12,940
+combined action of a platelet and fibrin web اللي
+
+528
+00:43:12,940 --> 00:43:17,360
+.. اللي .. الش .. اللي .. خليني أرجع لل slide
+
+529
+00:43:17,360 --> 00:43:23,500
+sorryالعمل أو ال .. ال .. ال action، الأثر تبع ال
+
+530
+00:43:23,500 --> 00:43:28,560
+blatlet، الصفائح الدموية و شبكة ال fibrin، تمام؟
+
+531
+00:43:28,560 --> 00:43:34,120
+هذا معناها أنه بيكونلي clot، هذه clot كفيلة بإنها
+
+532
+00:43:34,120 --> 00:43:38,500
+تمنعلي أنه يصير في عندي blood loss في جسم الإنسان
+
+533
+00:43:38,760 --> 00:43:43,760
+لإنها بت .. بتثبت الجلطة و بتسكر مكان الجروح و
+
+534
+00:43:43,760 --> 00:43:49,680
+بتظل موجودة لحد الالتئام لما بيصير الالتئام للجروح
+
+535
+00:43:49,680 --> 00:43:54,960
+و يصير فيه repair طبعا ل .. ال tissue ل ال .. ال
+
+536
+00:43:54,960 --> 00:43:59,800
+blood vessel طبعا يصير له repair يرجع يعني يتعمر و
+
+537
+00:43:59,800 --> 00:44:06,040
+يشتغل و يصير intact و يرجع سليم طبعا فهذه لحد ما
+
+538
+00:44:06,040 --> 00:44:11,250
+يصير فيه repair and healingوشفاء معناها ال .. ال
+
+539
+00:44:11,250 --> 00:44:14,810
+.. ال clot هي راح تظل موجودة و راح تظل تمنعلي ال
+
+540
+00:44:14,810 --> 00:44:18,550
+loss of blood اذا هذه ال clot ايش معناها؟ ممكن
+
+541
+00:44:18,550 --> 00:44:23,850
+تكون life saving يعني بتحمي الحياة احنا في ناس
+
+542
+00:44:23,850 --> 00:44:27,350
+كتير ممكن انهم يموتوا بسبب النزيف بسبب انهم هم
+
+543
+00:44:27,350 --> 00:44:32,110
+ماعندهم مش امكانية او الجسم هم مش قادر يوجف النزيف
+
+544
+00:44:32,110 --> 00:44:38,480
+هذا فالناس اللي عندهم impairedeclote formation
+
+545
+00:44:38,480 --> 00:44:41,380
+طبعا the formation of a clot is impaired او
+
+546
+00:44:41,380 --> 00:44:45,420
+impaired clot formation نتيجة انه مشكلة اما في
+
+547
+00:44:45,420 --> 00:44:50,920
+عوامل التخثر او مشكلة في البلاتلات هدول طبعا at
+
+548
+00:44:50,920 --> 00:44:53,980
+great risk of bleeding to death يعني ممكن يكون
+
+549
+00:44:53,980 --> 00:44:58,040
+عندهم خطر نظيف حتى الموت زي الناس اللي بتعاني من
+
+550
+00:44:58,040 --> 00:45:02,720
+المرض الوراثي ال hemophilia اللي بيكون فيه نقص في
+
+551
+00:45:02,720 --> 00:45:07,880
+عامل التخثر رقم تمانيةتمام؟ فرقم تمانية لما بي ..
+
+552
+00:45:07,880 --> 00:45:13,100
+مهم جدا العامل هذا في .. في السلسلة التخثر لحد ما
+
+553
+00:45:13,100 --> 00:45:17,260
+يتكون عندى ال fibrin إذا هذا العامل مش موجود معناه
+
+554
+00:45:17,260 --> 00:45:21,560
+أنا مش هحصل على fibrin فبالتالي ان ال .. اشخاص ..
+
+555
+00:45:21,560 --> 00:45:26,050
+فبالتالي ال .. ال ..ال .. ال platelet plug لحالها
+
+556
+00:45:26,050 --> 00:45:30,750
+اللي اتكونت مابتقدر انها تسد ال .. ال injury
+
+557
+00:45:30,750 --> 00:45:35,070
+فبالتالي مابتقدر توقف النزيف فبالتالي انه ممكن
+
+558
+00:45:35,070 --> 00:45:37,710
+يصير في عندهم نزيف حتى ال mouth احيانا في بعض
+
+559
+00:45:37,710 --> 00:45:42,350
+الجروح لازمإنه يعني يكون فيه .. طبعا ال blatelet
+
+560
+00:45:42,350 --> 00:45:48,210
+plug بتيجي موجودة بتسد المكان الجرح ولكن ال fibrin
+
+561
+00:45:48,210 --> 00:45:52,150
+clot أو ال fibrin web اللي هي شركة .. شبكة ال
+
+562
+00:45:52,150 --> 00:45:56,450
+fibrin بتيجي و بتتراكم على سطح ال blatelet أصلا هي
+
+563
+00:45:56,450 --> 00:45:59,430
+العوامل اللي بتتنشط على سطح ال blatelet فبتيجي
+
+564
+00:45:59,430 --> 00:46:05,480
+بتتراكم على سطح ال blatelet فبالتاليبت .. بتكونلي
+
+565
+00:46:05,480 --> 00:46:12,280
+شبكة و الشبكة هذه غير ذائبة و قوية فبتدعم و بتقوي
+
+566
+00:46:12,280 --> 00:46:16,480
+الجلطة لحد ما يصير في عندي repair and healing مثل
+
+567
+00:46:16,480 --> 00:46:22,320
+ما حكيناطيب إذا أنواع خلايا الدم erythrocyte خلايا
+
+568
+00:46:22,320 --> 00:46:27,100
+الدم الحمراء dyscotic at the top side وbiconcave
+
+569
+00:46:27,100 --> 00:46:35,520
+at the side view تمام وفي عندي خلايا دم حمراء إذا
+
+570
+00:46:35,520 --> 00:46:39,740
+المسئولة عن ال oxygen delivery في عندي خلايا الدم
+
+571
+00:46:39,740 --> 00:46:43,540
+البيضاء اللي هي عبارة عن lymphocytes خلايا ال
+
+572
+00:46:43,540 --> 00:46:47,150
+lymphocytes بأحجامهاخلايا ال monocytes اللي هي
+
+573
+00:46:47,150 --> 00:46:49,770
+عبارة عن إيش حكينا عن ال lymphocyte و ال monocyte
+
+574
+00:46:49,770 --> 00:46:55,050
+حكينا إنهم هم a granulocyte خلايا ال bezophil و ال
+
+575
+00:46:55,050 --> 00:46:57,930
+ezinophil و ال neutrophil هاي شايفين أنتوا
+
+576
+00:46:57,930 --> 00:47:01,270
+الحبيضات واضحة في ال cytoplasm تبعها فإذن هي خلايا
+
+577
+00:47:01,270 --> 00:47:08,410
+granulocyte بسميها طيب نيجي لإشي مهم اللي هو عبارة
+
+578
+00:47:08,410 --> 00:47:13,890
+عن ال anticoagulantكلمة coagulation لحالها هذه
+
+579
+00:47:13,890 --> 00:47:20,150
+coagulants يعني تجلط، تمام؟ anticoagulants يعني
+
+580
+00:47:20,150 --> 00:47:26,650
+عوامل ماني على التجلطهلأ إحنا في حصات اللي .. اللي
+
+581
+00:47:26,650 --> 00:47:31,830
+تجرى لتقييم محتويات الدم تمام؟ اللي بسميها ال
+
+582
+00:47:31,830 --> 00:47:35,530
+hematology and coagulation test أو hematology and
+
+583
+00:47:35,530 --> 00:47:38,670
+coagulation procedures الحصات اللي بتجرى عليها
+
+584
+00:47:38,670 --> 00:47:42,250
+عينة الدم لحتى أنا أقيم فيها مكونات الدم إما بدي
+
+585
+00:47:42,250 --> 00:47:45,930
+أعد خلايا الدم أو أشوف نسبة ال homoglobin في الدم
+
+586
+00:47:45,930 --> 00:47:49,670
+أو بدي أشوف نسبة عوامل التخثر في الدم أو نشاطها أو
+
+587
+00:47:49,670 --> 00:47:53,710
+تركيزها فهذه كلها اللي بتقيم لي وظائف الدم تمام؟
+
+588
+00:47:53,880 --> 00:47:58,340
+فبالتالي كلها تجرى على إيش؟ تجرى على عينة إما
+
+589
+00:47:58,340 --> 00:48:04,880
+اسمها whole blood يعني دم كامل تمام أو plasma أصبح
+
+590
+00:48:04,880 --> 00:48:07,980
+طالما .. طالما أنه أنا بدي أحصل على plasma إذا
+
+591
+00:48:07,980 --> 00:48:12,700
+لازم الدم اللي أنا بدي أسحبه من المريض إشماله
+
+592
+00:48:12,700 --> 00:48:18,320
+مايتجلطعشان أحصل على plasma طبعا أن الدم .. طبعا
+
+593
+00:48:18,320 --> 00:48:21,720
+الدم إحنا لو سحبناه و تركناه في انبوبة على طول
+
+594
+00:48:21,720 --> 00:48:25,620
+بيتجلط لحاله بتنشط .. تتنشط عوامل التخثر و بتجلط
+
+595
+00:48:25,620 --> 00:48:30,400
+طبعا؟ طيب ف .. لكن أنا بدي أسحبه و بدي أحصل على
+
+596
+00:48:30,400 --> 00:48:35,460
+plasma إذا لازم أضيفله مادة تمنع تجلطه علشان أنا
+
+597
+00:48:35,460 --> 00:48:41,360
+.. يعني ال .. أظلم حافظة على قوامه السائل و لما
+
+598
+00:48:41,360 --> 00:48:46,920
+أحصله أحصل عليه plasmaتمام؟ طيب إذا عشان ال ..
+
+599
+00:48:46,920 --> 00:48:50,600
+الفحوصات إما بتجري على whole blood أو ع plasma
+
+600
+00:48:50,600 --> 00:48:54,440
+يعني أنا بدي الدم يضل سائل عشان .. وضل كامل مخلوط
+
+601
+00:48:54,440 --> 00:48:58,020
+و كامل أو plasma يعني يضل سائل و أنا لما أفصل و
+
+602
+00:48:58,020 --> 00:49:02,580
+أحصل على السائل القشي اللي موجود at the top تمام؟
+
+603
+00:49:02,880 --> 00:49:06,700
+فبالتالي ال blood is withdrawn from the patient،
+
+604
+00:49:06,700 --> 00:49:11,960
+الدم اللي بيسحب من المريض لازم يخلط، يصيرله mixed
+
+605
+00:49:11,960 --> 00:49:15,220
+مع إيش؟ مع anticoagulant، أنا بدي أضيفله
+
+606
+00:49:15,220 --> 00:49:20,800
+anticoagulant، مادة أو عامل مانع للتخثر من كلمة ..
+
+607
+00:49:20,800 --> 00:49:25,200
+من كلمة anti يعني مضاد، تمام؟ coagulants يعني
+
+608
+00:49:25,200 --> 00:49:29,460
+تخثر، مضاد للتخثرعشان إيش أعمل؟ عشان prevent
+
+609
+00:49:29,460 --> 00:49:34,220
+coagulation أمنع تخثر الدم فبالتالي أظل محافظة على
+
+610
+00:49:34,220 --> 00:49:38,720
+الدم سائل فبالتالي أقدر أحصل على whole blood تمام
+
+611
+00:49:38,720 --> 00:49:42,540
+و أحصل على plasma تفيدني في الفحوصات اللي بدي
+
+612
+00:49:42,540 --> 00:49:47,980
+أعملهاتمام؟ أكثر تلت أنواع من ال anticoagulant
+
+613
+00:49:47,980 --> 00:49:52,120
+المستخدمة في مختبرات الهيماتولوجي في مختبرات الدم
+
+614
+00:49:52,120 --> 00:49:57,500
+هما اللي هما ال .. ال .. ال .. ال edita تمام؟ و ال
+
+615
+00:49:57,500 --> 00:50:02,520
+citrate sodium citrate و ال heparin خلينا نشوف إيش
+
+616
+00:50:02,520 --> 00:50:07,120
+مكونات أو إيش خصائص كل واحدة من هذه ال
+
+617
+00:50:07,120 --> 00:50:10,600
+anticoagulant أو المضادات التخطر أو موانع التخطر
+
+618
+00:50:11,140 --> 00:50:15,000
+طيب ال Edita هي عبارة طبعا بتكون احنا .. انا
+
+619
+00:50:15,000 --> 00:50:18,540
+بستخدم أملاح ال Edita طب ال Edita هي عبارة عن
+
+620
+00:50:18,540 --> 00:50:23,240
+اختصار ل إيش؟ اختصار ل ethylene E, ethylene,
+
+621
+00:50:23,680 --> 00:50:29,020
+diamine, tetra, acetic acidهي معنى Edita هيها
+
+622
+00:50:29,020 --> 00:50:33,640
+إثيليين ديامين تترا أسيتك أسد طبعا أنا حكيتلكم
+
+623
+00:50:33,640 --> 00:50:37,480
+باخد أملاح ال Edita يعني هذه ال .. ال إثيليين
+
+624
+00:50:37,480 --> 00:50:41,700
+ديامين تترا أسيتك أسد ال أسيتك أسد هذا بيكون مرتبط
+
+625
+00:50:41,700 --> 00:50:45,940
+بالآخر مع إيش إما مع ال Sodium أو Dipotassium أو
+
+626
+00:50:45,940 --> 00:50:51,290
+Tripotassium تمام؟إذا ال Edeta أنا باخد أملاحها
+
+627
+00:50:51,290 --> 00:50:54,390
+بستخدم الأملاح ال Edeta يعني يا إما ال Edeta دي
+
+628
+00:50:54,390 --> 00:50:57,570
+بتكون مرتبطة مع Sodium أو Di-Botassium أو Tri
+
+629
+00:50:57,570 --> 00:51:00,990
+-Botassium طب إيش هي ال Edeta؟ Ethylene, Diamine,
+
+630
+00:51:01,130 --> 00:51:05,230
+Tetraacetic acid بيكون غالبا تركيزها واحد و نص
+
+631
+00:51:05,230 --> 00:51:08,830
+plus minus ربع يعني من واحد و ربع لواحد point سبعة
+
+632
+00:51:08,830 --> 00:51:13,560
+خمسةتمام؟ هذا التركيز اللي .. اللي .. اللي بيستخدم
+
+633
+00:51:13,560 --> 00:51:19,500
+في المختبرات طبعا ال EDTA بتمنع التخثر من خلال ash
+
+634
+00:51:19,500 --> 00:51:22,380
+طبعا يا بنات احنا .. انا حكيتلكم في .. في عملية
+
+635
+00:51:22,380 --> 00:51:25,940
+التخثر الدم عندي سلسلة من البروتينات اللي بسميها
+
+636
+00:51:25,940 --> 00:51:30,460
+عوامل تخثر أو عوامل تجلط أو بروتينات تجلط بتتنشط
+
+637
+00:51:30,460 --> 00:51:35,140
+كسلسلة وفي المحصلة في النهاية اخر step فيها انه ال
+
+638
+00:51:35,140 --> 00:51:38,240
+fibrinogen بتتحول لfibrin عن طريق ال atherombin
+
+639
+00:51:39,110 --> 00:51:42,470
+تمام؟ هلأ التفاصيل هذه العملية كلها هتاخدوها في
+
+640
+00:51:42,470 --> 00:51:48,070
+مساق اسمه مساق علم النظف والتجلط لكن ..بس انتوا
+
+641
+00:51:48,070 --> 00:51:51,110
+معنيين انكوا تفهموا ان في بروتينات اسمها بروتينات
+
+642
+00:51:51,110 --> 00:51:54,710
+تخثر و بتتنشط كسلسلة يعني واحد بنشط التاني التاني
+
+643
+00:51:54,710 --> 00:51:58,270
+بنشط التالت التالت بنشط هكذا في المحصلة انا بحصل
+
+644
+00:51:58,270 --> 00:52:02,190
+على انه في النهاية عامل التجلط رقم اتنين اللي هو
+
+645
+00:52:02,190 --> 00:52:05,470
+thrombin بنشط عامل التجلط رقم واحد اللي هو ال
+
+646
+00:52:05,470 --> 00:52:08,350
+fibrinogen و بيحولوا ل fibrin ال fibrinogen
+
+647
+00:52:08,350 --> 00:52:14,050
+soluble لكن ال fibrin insoluble تمام؟ طيبهلأ هذه
+
+648
+00:52:14,050 --> 00:52:18,530
+البروتينات وهي عامالها بتنشط بعض بتكون محتاجة ل ..
+
+649
+00:52:18,530 --> 00:52:23,550
+ايونات الكالسيوم محتاجة لإيش؟ للكالسيوم ايون اللي
+
+650
+00:52:23,550 --> 00:52:29,690
+موجودة في الدم طيب لو انا حطي .. اضيفت للدم مادة
+
+651
+00:52:29,690 --> 00:52:35,430
+زي الاديتا تعتبر كمصيادة لالكالسيوم يعني بتاخدكل
+
+652
+00:52:35,430 --> 00:52:39,050
+.. تسحب كل ال calcium اللي موجود في العينة .. في
+
+653
+00:52:39,050 --> 00:52:43,770
+عينة الدم أصبح معناها عوامل التخثر مش راح تلاقي ال
+
+654
+00:52:43,770 --> 00:52:48,130
+.. ال .. ال .. ال calcium كعامة .. ك co-factor
+
+655
+00:52:48,130 --> 00:52:53,650
+لإلها فبالتالي مش هتتنشط تمام؟ فمش هتنشط بعضها
+
+656
+00:52:53,650 --> 00:52:57,370
+فبالتالي مش ه .. في المحصلة مش هيتكون ال fibrin
+
+657
+00:52:57,370 --> 00:53:01,750
+فبالتالي مش راح يتخسر الدم تمام؟ إذا كيف ال Edita
+
+658
+00:53:01,750 --> 00:53:08,650
+بتمنعإنها .. إن الدم يتخثر عن طريق إنها ترتبط مع
+
+659
+00:53:08,650 --> 00:53:11,910
+ال calcium اللي موجود في الدم ال calcium ضروري
+
+660
+00:53:11,910 --> 00:53:16,510
+required for blood coagulation ضروري بيلزم عوامل
+
+661
+00:53:16,510 --> 00:53:20,820
+التخثر و هي عمالها بتنشف بعضهامافيش calcium عوامل
+
+662
+00:53:20,820 --> 00:53:24,220
+التخفر مش راح تتنشط ولا تتنشط بعضها فبالتالي مش
+
+663
+00:53:24,220 --> 00:53:27,060
+راح يصير فيه coagulation في الدم فبالتالي راح يظل
+
+664
+00:53:27,060 --> 00:53:31,420
+الدم سائل فبالتالي طالما انه ظل الدم حافظ على
+
+665
+00:53:31,420 --> 00:53:35,960
+قوامه سائل معناه انا بقدر احصل على whole blood ل
+
+666
+00:53:35,960 --> 00:53:40,300
+الفحوصات و بقدر انا لو حطته ع جهاز ال centrifuge
+
+667
+00:53:40,300 --> 00:53:44,140
+وفصلته السائل القشي اللي موجود at the top طالما
+
+668
+00:53:44,140 --> 00:53:47,320
+الدم مش متجلة اسمه plasma اذا بينفعني في الفحوصات
+
+669
+00:53:47,320 --> 00:53:54,160
+الهيماتولوجيةتمام؟ طيب خلينا نشوف قبل أحكي عن هذه
+
+670
+00:53:54,160 --> 00:53:57,640
+النقطة إيش هي .. كيف شكل الأنابيب اللي أنا بجمع
+
+671
+00:53:57,640 --> 00:54:02,940
+فيها عينة الدم خلينا نشوف هاي هنا ال tube of blood
+
+672
+00:54:02,940 --> 00:54:07,340
+هذه طبعا أنابيب مختلفة فيها anticoagulant مختلف
+
+673
+00:54:07,340 --> 00:54:13,220
+تمام؟ و نجمع فيها عينة الدم كل انبوب بتكون متخصصة
+
+674
+00:54:13,220 --> 00:54:19,120
+لفحص معينمثلا في عندى بيكون سترات في بيكون ال ..
+
+675
+00:54:19,120 --> 00:54:26,720
+heparin هى edita هى طبعا لل ESR سترات و هى هنا فيه
+
+676
+00:54:26,720 --> 00:54:30,400
+plain يعنى مافيش فيها حاجة خالص طبعا فاضية يعنى
+
+677
+00:54:30,400 --> 00:54:33,520
+الدم هيتجلط فيها مدى مافيش فيها anticoagulant الدم
+
+678
+00:54:33,520 --> 00:54:36,680
+هيتجلط فيها و لما أفصلها في ال centrifuge هتعطينى
+
+679
+00:54:36,680 --> 00:54:40,240
+serum مش plasma طبعا اذا هذه هي الأنابيب اللي هى
+
+680
+00:54:40,240 --> 00:54:44,890
+color .. color code يعنى كل لونعبارة بيدللي إن
+
+681
+00:54:44,890 --> 00:54:48,810
+محتوياته مختلفة تمام في ال anticoagulant اللي
+
+682
+00:54:48,810 --> 00:54:52,370
+موجود فيه مختلف إما بيكون فيها anticoagulant مختلف
+
+683
+00:54:52,370 --> 00:54:55,570
+أو بيكونش فيها anticoagulant خالص زي البلان هاي
+
+684
+00:54:55,570 --> 00:55:00,050
+الحمرة تمام فبالتالي إما بتفصللي plasma أو بتفصللي
+
+685
+00:55:00,050 --> 00:55:04,730
+serum لما أحطها على جهاز ال centrifuge فبالتالي
+
+686
+00:55:04,730 --> 00:55:09,510
+حسب إيش مكوناتها أو إيش محتويات ال tubeتمام؟ يا
+
+687
+00:55:09,510 --> 00:55:12,770
+بنات كمية ال anticoagulant اللي موجودة أحيانا
+
+688
+00:55:12,770 --> 00:55:18,700
+بتكون يعني لا تكتت الكارويعني أحيان ما بتشوفيها ..
+
+689
+00:55:18,700 --> 00:55:21,860
+ممكن تشوفيها ك powder أو تشوفيها كقطرات على جدار
+
+690
+00:55:21,860 --> 00:55:25,640
+التيوب و ممكن ما تشوفيها، ما تلاحظيها، لكن هي
+
+691
+00:55:25,640 --> 00:55:29,100
+بيكون موجودة فيها كميات قليلة من ال anticoagulant
+
+692
+00:55:29,100 --> 00:55:33,940
+و بالتالي أيضا مابيكون معلملي أنه أنا أسحب الدم
+
+693
+00:55:33,940 --> 00:55:36,560
+لعند علامة معينة في التيوب هذه، يعني أنا لو بدي
+
+694
+00:55:36,560 --> 00:55:41,760
+أفض التيوب داخل أو أفض blood داخل التيوب، بدي
+
+695
+00:55:41,760 --> 00:55:46,130
+أفضيه عند علامة معينةبحيث أنه يظل نسبة الدم لل
+
+696
+00:55:46,130 --> 00:55:48,890
+anticoagulant ثابتة مائية يعني ال anticoagulant
+
+697
+00:55:48,890 --> 00:55:53,250
+هذا ملح بيأثر أحيانا على شكل الخلايا بيأثر على
+
+698
+00:55:53,250 --> 00:55:56,370
+المكونات
+
+699
+00:55:56,370 --> 00:56:01,370
+الدم فبالتالي لازم أنا أظلم حافظة على النسبة ما
+
+700
+00:56:01,370 --> 00:56:04,830
+بين الدم و عامل ال anticoagulant هذا بحيث أني
+
+701
+00:56:04,830 --> 00:56:08,490
+ماأثر على محتويات الدم ببالتالي ماأحصل على نتائج
+
+702
+00:56:08,490 --> 00:56:12,190
+خاطئة في الفحوصات يعني مثلا هيهانةلـ tube هذه ال
+
+703
+00:56:12,190 --> 00:56:15,890
+tube ال ESR فيها Sodium Citrate تمام ال .. ال
+
+704
+00:56:15,890 --> 00:56:18,310
+anticoagulant اللي موجود اسمه Sodium Citrate
+
+705
+00:56:18,310 --> 00:56:22,010
+هنشتغل فحص ال ESR و هنحكي عن Sodium Citrate
+
+706
+00:56:22,010 --> 00:56:26,410
+Anticoagulant و هيتكرر على ما سامعكم هذا الاسم
+
+707
+00:56:26,410 --> 00:56:30,920
+كتير Sodium Citrate تمام؟طيب شايفين العلامة هذه
+
+708
+00:56:30,920 --> 00:56:36,140
+اللي رونها أسود هذه لحد عندها لازم أنا أعبي blood
+
+709
+00:56:36,140 --> 00:56:40,580
+يعني لحد هنا أعبي blood عشان مايكونش كمية ال ..
+
+710
+00:56:40,580 --> 00:56:44,820
+السترات كمية الملح أكتر في الدم فبالتالي تأثر على
+
+711
+00:56:44,820 --> 00:56:49,680
+شكل الخلايا ومكونات الدماحيانا بيكون إذا ما أعطاني
+
+712
+00:56:49,680 --> 00:56:53,300
+علامة ع الجانب ممكن يكون هذا الخط الملون هو .. هو
+
+713
+00:56:53,300 --> 00:56:57,400
+نفس العلامة تمام؟ هذه شايفين إيش فيها زي الشمع أو
+
+714
+00:56:57,400 --> 00:57:01,820
+زي ال .. ال silicon زي مادة ال silicon جوا؟ هذه
+
+715
+00:57:01,820 --> 00:57:06,080
+بيكون فيها clot activator يعني بتعطيني serum تمام؟
+
+716
+00:57:06,080 --> 00:57:10,220
+بت .. بس إيش .. يعني .. يعني الدم بيتخسر أسرع من
+
+717
+00:57:10,220 --> 00:57:12,640
+هذه هذا الدم .. هذه مافيش فيها حاجة يعني أنا بترك
+
+718
+00:57:12,640 --> 00:57:16,000
+الدم يتخسر على أحتهبعدين لما أفصل في جهاز ال
+
+719
+00:57:16,000 --> 00:57:22,700
+centrifuge بيعطيني ash المكونات انه عبارة عن يعني
+
+720
+00:57:22,700 --> 00:57:27,810
+دم متجلة و أتدى top serumها دي نفس الاشي بيتجلط
+
+721
+00:57:27,810 --> 00:57:32,610
+الدم و لما أفصل بجهاز ال centrifuge بيعطيني ال
+
+722
+00:57:32,610 --> 00:57:36,990
+serum و الدم المتجلط بالأسفل ولكن هنا بيتجلط أثر
+
+723
+00:57:36,990 --> 00:57:41,230
+على إن هذا مادة ال silicon بقرا عن clock activator
+
+724
+00:57:41,230 --> 00:57:47,950
+يعني منشطة لحدوث التخثر تمام؟طيب فإذا بديكم بس
+
+725
+00:57:47,950 --> 00:57:52,790
+تركزوا كتير منيح إنه حجم الدم هنا بيكون طبعا في
+
+726
+00:57:52,790 --> 00:57:57,670
+عندي علامة بتكون موجودة لتحد تتحددلي أنا عبي ال
+
+727
+00:57:57,670 --> 00:58:02,490
+tube هاي ل .. ل .. لامتة أو ل .. لوين في ال blood
+
+728
+00:58:02,490 --> 00:58:06,150
+تمام؟ إما بيكون علامة على الجانب أو الخط الملون
+
+729
+00:58:06,150 --> 00:58:10,990
+هذا نفسه بيكون هو نفس العلامة طيب خلينا نشوف طيب
+
+730
+00:58:10,990 --> 00:58:16,450
+لو أناكان في عندي في ال tube تبعتي excessive
+
+731
+00:58:16,450 --> 00:58:20,450
+concentration of edita اش يعني؟ يعني ال edita
+
+732
+00:58:20,450 --> 00:58:26,190
+زيادة يعني الدم انا سحبته اقل من العلامة طبعا؟
+
+733
+00:58:26,190 --> 00:58:30,690
+مدام في عندي ملح كتير هذا هيأثر على شكل خلايا ال
+
+734
+00:58:30,690 --> 00:58:35,370
+RBCs ويعمللها shrinkage اش يعني shrinkage؟ يعني
+
+735
+00:58:35,370 --> 00:58:42,150
+الخلايا هتتكرمش هيصيرلها كرمشة طبعا؟ و تتقلص
+
+736
+00:58:43,060 --> 00:58:47,200
+فبالتالي هذا هيأثر على الفحوصات الهيماتوكرت هيخليه
+
+737
+00:58:47,200 --> 00:58:54,460
+decreased و هيخلي ال MCHC increased و هيقلل ال ESR
+
+738
+00:58:54,460 --> 00:59:00,120
+تمام؟ كل الفحوصات اللي بتعتمد على حجم الخلايا الدم
+
+739
+00:59:00,120 --> 00:59:03,740
+الحمراء على قياس حجم خلايا الدم الحمراء كلها راح
+
+740
+00:59:03,740 --> 00:59:09,510
+تتأثرdecreased hematocrit و increased MCHC و
+
+741
+00:59:09,510 --> 00:59:13,550
+falsely low E-ASR هدى طبعا اشملها هدى نتائج خاطئة
+
+742
+00:59:13,550 --> 00:59:18,130
+انا حصلت عليها نتيجة ان انا عبت ال blood اقل من
+
+743
+00:59:18,130 --> 00:59:21,890
+العلامة داخل التيوب يعنى انا ماحطت كفاية blood
+
+744
+00:59:21,890 --> 00:59:25,470
+داخل التيوب فبالتالي كمية الادتة كانت زيادة
+
+745
+00:59:25,470 --> 00:59:30,000
+بالنسبة لا ل bloodو أيضا الidity هيالزيادة ممكن
+
+746
+00:59:30,000 --> 00:59:32,780
+تعملي degenerative changes in white cells يعني
+
+747
+00:59:32,780 --> 00:59:37,480
+ممكن تخلي ال white cells انها هي تتحلل ممكن يعملي
+
+748
+00:59:37,480 --> 00:59:43,220
+swelling ل البلاتلت فبالتالي هي ايضا هيخليلي
+
+749
+00:59:43,220 --> 00:59:46,500
+هيعملي falsely و يخليها طبعا لما انها صار لها
+
+750
+00:59:46,500 --> 00:59:51,220
+swelling البلاتلت لما انها انتفخت ممكن تتكسر و لما
+
+751
+00:59:51,220 --> 00:59:58,170
+انا قاعدها على الجهازمفوتات ل blatlet يعدوا الجهاز
+
+752
+00:59:58,170 --> 01:00:02,870
+ك blatlet فبالتالي انه يعملي increase ان blatlet
+
+753
+01:00:02,870 --> 01:00:11,050
+count طبعا؟ طيبSodium citrate النوع التاني اللي هو
+
+754
+01:00:11,050 --> 01:00:15,350
+ال common يعني أكتر شيوعا في المختبرات ال
+
+755
+01:00:15,350 --> 01:00:19,330
+hematology هو عبارة عن Sodium citrate طبعا ملح
+
+756
+01:00:19,330 --> 01:00:25,530
+Sodium citrate بيكون إما موجود ك Sodium citrate
+
+757
+01:00:25,530 --> 01:00:30,930
+dehydrate أو Sodium citrate عادي طبعا طيب فبالتالي
+
+758
+01:00:30,930 --> 01:00:34,810
+أو potassium citrate أحيانافال .. ال sodium
+
+759
+01:00:34,810 --> 01:00:39,150
+citrate هذا دايما انا بحصل .. يعني طبعا لإذا بدي
+
+760
+01:00:39,150 --> 01:00:43,290
+اعمل فحوصات التخثر ال coagulation studies لازم
+
+761
+01:00:43,290 --> 01:00:48,990
+يكون نسبة الدم تسعة أجزاء و ال citrate one يعني
+
+762
+01:00:48,990 --> 01:00:56,120
+نسبة ال anticoagulant لل blood واحد لتسعةتمام؟
+
+763
+01:00:56,120 --> 01:01:00,020
+فإذا مهم جدا حكاية العلامة هذه وكمية الدم اللي أنا
+
+764
+01:01:00,020 --> 01:01:03,700
+بدي أفضيه داخل ال tube بعد ما أسحب العينة Sodium
+
+765
+01:01:03,700 --> 01:01:08,200
+citrate prevent coagulation by binding the calcium
+
+766
+01:01:08,200 --> 01:01:12,020
+to the blood in soluble complex طبعا احنا حكينا
+
+767
+01:01:12,020 --> 01:01:15,660
+انه هذا النوع من ال anticoagulant زي و زي ال Edeta
+
+768
+01:01:15,660 --> 01:01:19,500
+بيمنع التخطر من خلال ارتباطه بال calcium واحنا
+
+769
+01:01:19,500 --> 01:01:24,010
+حكينا انه ال calcium ضروريلتنشيط عوامل التخطر فإذا
+
+770
+01:01:24,010 --> 01:01:27,890
+مافيش calcium معناها عوامل التخطر مش روحت اتنشط
+
+771
+01:01:27,890 --> 01:01:31,750
+فمافيش كلوت فبالتالي الدم رح يظل سائل فإذا عشان
+
+772
+01:01:31,750 --> 01:01:35,950
+هيك بسمي هذه المواد anticoagulant مضادات للتخثر
+
+773
+01:01:35,950 --> 01:01:40,460
+موانع للتخثرتروح ترتبط بالكالسيوم و بتمنع التخثر
+
+774
+01:01:40,460 --> 01:01:46,420
+طيب TND الهيبرين نوع تالت من ال anticoagulant اللي
+
+775
+01:01:46,420 --> 01:01:52,100
+أكتر شيوعا في ال hematology lab ولكن طبعا انا ممكن
+
+776
+01:01:52,100 --> 01:01:58,830
+استخدمها من 15 ل 30 unit per ml تركيزهو طبعا ممكن
+
+777
+01:01:58,830 --> 01:02:01,670
+أحيانا يسببلي clumping ل ال blatelet في ناس عندهم
+
+778
+01:02:01,670 --> 01:02:05,630
+حساسية من ال heparin فبالتالي ممكن أنا إذا ضفتلهم
+
+779
+01:02:05,630 --> 01:02:09,750
+heparin لعينتهم يصير فيه clumping يصير فيه تجمعات
+
+780
+01:02:09,750 --> 01:02:15,650
+ل ال blatelet و ال white pieces فبالتالي هدول
+
+781
+01:02:15,650 --> 01:02:19,970
+الناس أنا إذا لاحظت إنه استخدمت ال anticoagulant
+
+782
+01:02:19,970 --> 01:02:23,630
+هذا ال heparin و روحت أعد خلايا ال blatelet و أعد
+
+783
+01:02:23,630 --> 01:02:26,610
+ال white pieces و لاجتها جليلةبدو يجي في بالي ان
+
+784
+01:02:26,610 --> 01:02:29,230
+هو ممكن يكون هذا المريض بيتحسس من الهيبارين
+
+785
+01:02:29,230 --> 01:02:32,750
+فبالتالي عشان هيك صار في عنده خلايا ال .. ال
+
+786
+01:02:32,750 --> 01:02:35,750
+platelet و ال white بيصير صارلها clumping فبالتالي
+
+787
+01:02:35,750 --> 01:02:39,390
+لازم انا ابدل يعني اصحبله عينة تانية و أحطها في
+
+788
+01:02:39,390 --> 01:02:44,010
+anticoagulant أخر بعدين أعمل count تمام؟ طيب هذه
+
+789
+01:02:44,010 --> 01:02:48,670
+يعني بس يعني من ال limitation او من عيوب الهيبارين
+
+790
+01:02:49,160 --> 01:02:53,540
+طيب ال heparin بيمنع التخثر أيضا، اذا انا ممكن
+
+791
+01:02:53,540 --> 01:02:57,180
+احصل على plasma من عينة الدم اللي مضعفة لهيبارين،
+
+792
+01:02:57,180 --> 01:03:01,860
+ولكن القالية تبعته مختلفة، احنا حاكينا ال Edeta و
+
+793
+01:03:01,860 --> 01:03:06,840
+ال citrate بيرتبطوا بال calcium، طبعا؟ فبيمنع ال
+
+794
+01:03:06,840 --> 01:03:11,340
+.. ال .. ان تنشيط عوامل التخثر، فبالتالي مابيصيش
+
+795
+01:03:11,340 --> 01:03:16,300
+فيه clotطبعا لكن الهيبارين إيش بيروح بعمل بيروح
+
+796
+01:03:16,300 --> 01:03:21,100
+بيشتغل بقالية مختلفة بميكانيزم مختلف الهيبارين
+
+797
+01:03:21,100 --> 01:03:26,340
+بيروح بنشط ال protein اسمه ال antithrombin 3 و هذا
+
+798
+01:03:26,340 --> 01:03:30,420
+ال antithrombin 3 بيروح بيرتبط مع ال thrombin و
+
+799
+01:03:30,420 --> 01:03:34,340
+بعمله inhibition طبعا فطالما ارتبط مع ال thrombin
+
+800
+01:03:34,340 --> 01:03:38,480
+و عملله inhibition مافيش عندي fibrinogen يتحول ل
+
+801
+01:03:38,480 --> 01:03:42,520
+fibrin إذا مافيش عندي clot إذا ال blood هيظل سائل
+
+802
+01:03:42,610 --> 01:03:47,570
+طبعا هذا هو الفارق في القالية ما بين ال .. ال
+
+803
+01:03:47,570 --> 01:03:51,960
+اليتا و الستريات و ما بين الهيبارينطيب ال blood
+
+804
+01:03:51,960 --> 01:03:56,980
+collecting tubes طبعا احنا في عندى عدة انواع من ال
+
+805
+01:03:56,980 --> 01:03:59,780
+tubes اللى هى ال blood collecting tubes هى هنا فيه
+
+806
+01:03:59,780 --> 01:04:02,500
+several types of blood collecting tubes are
+
+807
+01:04:02,500 --> 01:04:08,560
+available و طبعا حكينا انه هى ال .. ال .. ممكن انا
+
+808
+01:04:08,560 --> 01:04:11,760
+اميزها discriminated by their top color حكينا
+
+809
+01:04:11,760 --> 01:04:16,610
+انهيلها color codeكل لون الغطاية أو اللون ال .. ال
+
+810
+01:04:16,610 --> 01:04:20,790
+top تبعها مختلف فبيكون .. كل لون بيعطي .. بي ..
+
+811
+01:04:20,790 --> 01:04:24,630
+بيدللي إن ال .. ال .. ال anticoagulantilli في
+
+812
+01:04:24,630 --> 01:04:30,250
+الداخل مختلفتمام؟ يعني هاي ال .. ال .. ال cube
+
+813
+01:04:30,250 --> 01:04:34,550
+اللي ال top تبعها red طبعاً هذا يا بناتي يعني إشي
+
+814
+01:04:34,550 --> 01:04:38,530
+معمّم، ما .. يعني مابنفعش .. يعني مش مطبق في دولة
+
+815
+01:04:38,530 --> 01:04:43,190
+او دولة لأ، لأ هي كل الأنابيب هيك بتكون، تمام؟
+
+816
+01:04:43,190 --> 01:04:47,330
+فبالتاليالقنابيب اللي ال red .. ال .. ال top تبعها
+
+817
+01:04:47,330 --> 01:04:50,510
+لونه red حكينا إيش بسميها plain tube يعني مافيش
+
+818
+01:04:50,510 --> 01:04:57,410
+فيها أي حاجة فاضية no أو none additive تمام؟ فهذه
+
+819
+01:04:57,410 --> 01:05:01,390
+ال non additive إيش يعني؟ يعني مافيش فيها أي
+
+820
+01:05:01,390 --> 01:05:07,210
+إضافات فالدم هيفصل و هيتجلط و لما أفصل و هيعطيني
+
+821
+01:05:07,210 --> 01:05:11,490
+serum تمام؟ في عندي ال .. ال top اللي لونه orange
+
+822
+01:05:11,490 --> 01:05:18,140
+في .. خلينا نشوف .. نرجع لصورة هنااللي لونه هيك
+
+823
+01:05:18,140 --> 01:05:22,980
+على orange أو على أصفر فيها clot activator تمام؟ و
+
+824
+01:05:22,980 --> 01:05:27,680
+هذا ليه .. ليه clot activator؟ يعني منشط للتخثر
+
+825
+01:05:27,680 --> 01:05:32,660
+فبرضه ال blood هيتجلط فيه و هيعطيني serum و أيضا
+
+826
+01:05:32,660 --> 01:05:36,980
+نفس الاشي اللي .. اللي لونه yellow اللي لونه أصفر
+
+827
+01:05:36,980 --> 01:05:42,300
+صريح برضه كمان فيه clot activator و فيه gelفهدول
+
+828
+01:05:42,300 --> 01:05:47,020
+برضه كمان بينشطوا عملية التجلط ف ال blood هيتجلط و
+
+829
+01:05:47,020 --> 01:05:51,460
+لما أفصله هيعطيني serum تمام؟ ال heparin tube
+
+830
+01:05:51,460 --> 01:05:55,260
+بيكون لونها green في هذا اللون، لون ال mint green
+
+831
+01:05:55,260 --> 01:05:58,960
+اللي هو الأخضر اللي .. اللي ماخد اللون النعناعي
+
+832
+01:05:58,960 --> 01:06:05,100
+تمام؟ heparin tube فهذا ال .. ال tube فيه heparin
+
+833
+01:06:05,100 --> 01:06:08,320
+إذا الدم مش هيتجلط إذا لما أفصله بيعطيني plasma
+
+834
+01:06:08,930 --> 01:06:12,930
+تمام؟ إما heparin sodium أو heparin lithium بيكون
+
+835
+01:06:12,930 --> 01:06:16,970
+فيه .. تمام؟ اللي لونه light green الأخضر الفاتح
+
+836
+01:06:16,970 --> 01:06:21,410
+بيكون موجود فيه gel و heparin lithium تمام؟ و بي
+
+837
+01:06:21,410 --> 01:06:25,590
+.. و .. و بيفصل ال .. طبعا .. طبعا الدم مش هيتخسر
+
+838
+01:06:25,590 --> 01:06:29,030
+مدام فيه heparin فبالتالي لما أفصله بيعطيني plasma
+
+839
+01:06:29,030 --> 01:06:33,490
+إيش يعني plasma؟ يعني أن السائل ما .. ما .. ما تم
+
+840
+01:06:33,490 --> 01:06:37,250
+استهلاكال .. العوامل التخطرى اللى موجودة فى الدم
+
+841
+01:06:37,250 --> 01:06:41,290
+فالسائل القشى اللى موجود فوق ال blood هذا محتوى ..
+
+842
+01:06:41,290 --> 01:06:44,890
+بيحتوي على fibrinogen و لو أنا عملت mix للعينة
+
+843
+01:06:44,890 --> 01:06:49,570
+بترجع دم، بترجع whole blood تمام؟ ال Edita تيوب
+
+844
+01:06:49,570 --> 01:06:55,850
+اللى لونها .. يعني بيكون لونها purple أو ..move او
+
+845
+01:06:55,850 --> 01:06:59,170
+حتى lavender احيانا بيسموها في كتب بتسميها اللون
+
+846
+01:06:59,170 --> 01:07:02,650
+ال lavender فبالتالي هذه اما dibotassium او
+
+847
+01:07:02,650 --> 01:07:07,670
+tributassium او حتى ال sodium edita هذه كلها بتكون
+
+848
+01:07:07,670 --> 01:07:11,310
+بتعطيني اما holy blood او لو فصلتها بتعطيني plasma
+
+849
+01:07:11,310 --> 01:07:15,920
+تمام؟ال blue بيكون فيها sodium citrate بتركيز
+
+850
+01:07:15,920 --> 01:07:20,580
+مختلفة عشان تعطيني ال .. طبعا انا باخد ال plasma
+
+851
+01:07:20,580 --> 01:07:24,660
+تبعتها و بشتغل عليها بحوصات التخثر ال tube اللي
+
+852
+01:07:24,660 --> 01:07:26,820
+بيكون ال tube تبعها black بيكون فيها sodium
+
+853
+01:07:26,820 --> 01:07:31,220
+citrate عشان اشتغل فيها فحص ال ESR و ال glucose
+
+854
+01:07:31,220 --> 01:07:35,040
+tube اللي هي لونها greyبيكون فيها Sodium Fluoride
+
+855
+01:07:35,040 --> 01:07:40,360
+طبعا ال Sodium Fluoride من خصائصه انه هو بيمنع ال
+
+856
+01:07:40,360 --> 01:07:43,200
+glycolysis تبع الخلايا بيمنع ال metabolism تبع
+
+857
+01:07:43,200 --> 01:07:46,720
+الخلايا فما انه بيمنع ال .. ال metabolism تبع ..
+
+858
+01:07:46,720 --> 01:07:50,060
+هو inhibitor للميطابوليزم و بيمنع الميطابوليزم
+
+859
+01:07:50,060 --> 01:07:54,890
+فبالتالي الخلايا مش راح تستهلك glucoseفإذا يفضل
+
+860
+01:07:54,890 --> 01:07:58,630
+إنه أنا لو بدي أشتغل فحص ل glucose أضيء .. أسحب
+
+861
+01:07:58,630 --> 01:08:02,550
+عينة الدم و أضيفها على tube ال top تبعها grey يعني
+
+862
+01:08:02,550 --> 01:08:08,530
+موجود فيها sodium و fluoride زي هذا الشكل هذاتمام؟
+
+863
+01:08:08,530 --> 01:08:11,250
+هي موجودة .. هي مكتوبة هنا glucose يعني فيها
+
+864
+01:08:11,250 --> 01:08:15,750
+Sodium Fluoride تمام؟ هذه هي مكونات .. هذا هو ال
+
+865
+01:08:15,750 --> 01:08:20,010
+color code و هذا المكونات اللي فيها .. الإضافات
+
+866
+01:08:20,010 --> 01:08:23,670
+اللي فيها و هذا نوع العينة اللي بحصل عليه من كل
+
+867
+01:08:23,670 --> 01:08:29,630
+tube الجدول هذا يا بنات مش حفظ رح يكون معاكم طبعا
+
+868
+01:08:29,630 --> 01:08:33,050
+يعني بعض الأشياء حفظ يعني مهم تعرفوا لإن ال planet
+
+869
+01:08:33,050 --> 01:08:35,890
+tube مافيش فيها حاجة و بتعطيني serum يعني الدم
+
+870
+01:08:35,890 --> 01:08:40,780
+بيتخسرال black tube فيها sodium citrate و بتشتغل
+
+871
+01:08:40,780 --> 01:08:44,360
+.. بتنشغل لل ESR ال .. ال .. ال bare ball فيها
+
+872
+01:08:44,360 --> 01:08:48,540
+edita و بتعطيني holy blood و لو فصلتي ال holy
+
+873
+01:08:48,540 --> 01:08:52,940
+blood بحصل على plasma باقي الألوان مع الممارسة و
+
+874
+01:08:52,940 --> 01:08:57,870
+مع التكرار هيمروا معاكم خلال فحوصات ال ..كتيرة في
+
+875
+01:08:57,870 --> 01:09:03,770
+مختبرات التحليل فبالتالي ما بتتخرجوا إلا أنتوا
+
+876
+01:09:03,770 --> 01:09:07,470
+بتعرفوا هاي الأنابيب و بتعرفوا إيش مكوناتها و يمكن
+
+877
+01:09:07,470 --> 01:09:12,470
+حاقظين ألوانها من كتر ما بتستخدموها، تمام؟ فإذا بس
+
+878
+01:09:12,470 --> 01:09:17,950
+اعرفولي حاليا مبدأيا في المعمل تبعنا هذا ال red ال
+
+879
+01:09:17,950 --> 01:09:22,640
+purple ال black تمام؟فهدول بس اللي بتعرفوا .. بدكم
+
+880
+01:09:22,640 --> 01:09:26,280
+تعرفوا ليهم ال verbal ممكن تلاقوا في كتب بتعطيني
+
+881
+01:09:26,280 --> 01:09:30,520
+ان هو اسمه lavender تمام؟ هتمروا يعني معانا
+
+882
+01:09:30,520 --> 01:09:33,920
+chapter بيحكي عن ال verbal هذا او ال .. التيوب هاي
+
+883
+01:09:33,920 --> 01:09:39,520
+lavender فهي نفس المعنى تمام؟ فهدول اللي ..
+
+884
+01:09:42,220 --> 01:09:45,880
+المطالبين أنتوا تحفظوهم طبعا كل cube يا بنات في
+
+885
+01:09:45,880 --> 01:09:49,700
+منها أحجام في بتسحب ..يعني بتكفي لحد اتنين أمقال
+
+886
+01:09:49,700 --> 01:09:53,080
+لتلاتة لاربع لخمسة أمقال طبعا اللي بيحددلي ال
+
+887
+01:09:53,080 --> 01:09:56,300
+volume اللي أنا بدي أعبيه هو العلامة اللي وين
+
+888
+01:09:56,300 --> 01:10:01,220
+معلمة أو اللي وين موجود في علامة داخلال .. ال ..
+
+889
+01:10:01,220 --> 01:10:04,740
+او على .. على جدار ال tube إما على الجانب أو حتى
+
+890
+01:10:04,740 --> 01:10:08,160
+في .. يعني هذا أحيانا بيكون هو نفسه علامة فلوين في
+
+891
+01:10:08,160 --> 01:10:12,780
+علامة عندي، لوين بعبي أنا blood فبالتالي بيحددلي
+
+892
+01:10:12,780 --> 01:10:16,000
+ال volume، طبعا، في tubeات بتكون مصممة ل volume
+
+893
+01:10:16,000 --> 01:10:20,700
+قليل، في tubeات بتكون مصممة ل volume أكترحسب نوع
+
+894
+01:10:20,700 --> 01:10:26,220
+الفحص أو الفحوصات اللي مطلوبة، يعني إذا أنا بدي ..
+
+895
+01:10:26,220 --> 01:10:29,400
+بدي أشتغل من ال YouTube هذي عدة فحوصات، بختار ال
+
+896
+01:10:29,400 --> 01:10:32,740
+YouTube اللي حجم .. بتعطيني حجم أكتر، أما إذا كانت
+
+897
+01:10:32,740 --> 01:10:36,060
+ال .. الفحوصات قليلة فبختار ال YouTubeات اللي بت
+
+898
+01:10:36,060 --> 01:10:40,760
+.. بتعبق أو بيكون فيهاأو بتعطيني حجم أقل هذا
+
+899
+01:10:40,760 --> 01:10:45,660
+بالنسبة لمحاضرتنا لليوم بنكون هيك خلصنا تعرفنا على
+
+900
+01:10:45,660 --> 01:10:50,420
+مكونات الدم و ال anticoagulant و الأنواع الأكتر
+
+901
+01:10:50,420 --> 01:10:55,780
+common و وظائفها تمام؟عندكم أي سؤال، أي استفسار
+
+902
+01:10:55,780 --> 01:11:00,660
+بعتوليا بقى على group ال whatsapp بجاوبكم من خلال
+
+903
+01:11:00,660 --> 01:11:05,700
+تسجيل صوتي أو بنتناقش فيه عبر محاضرة ال zoom اللي
+
+904
+01:11:05,700 --> 01:11:10,440
+موعدها يوم الصبت يعطيكم العافية وموفقين بإذن الله
+
diff --git a/PL9fwy3NUQKwY9jl8xw-bWkVZkWcGGyspd/mJoJF0VELyo_raw.json b/PL9fwy3NUQKwY9jl8xw-bWkVZkWcGGyspd/mJoJF0VELyo_raw.json
new file mode 100644
index 0000000000000000000000000000000000000000..1610369b1e16beaeba2b10e6e014280883b7921d
--- /dev/null
+++ b/PL9fwy3NUQKwY9jl8xw-bWkVZkWcGGyspd/mJoJF0VELyo_raw.json
@@ -0,0 +1 @@
+{"segments": [{"id": 1, "seek": 3099, "start": 4.39, "end": 30.99, "text": "السلام عليكم ورحمة الله وبركاته اليوم ان شاء الله رح نبدأ محاضرة جديدة بعنوان blood components مكونات الدم بالبداية ما هو تعريف الدم؟ ما هو ال blood؟ تمام ال blood أو الدم هو عبارة عن السائل الأحمر the red fluid that oozes out of your body when you have sustained a cut or deep injury", "tokens": [6027, 3794, 37440, 25894, 24793, 4032, 2288, 35571, 3660, 21984, 4032, 26890, 4117, 9307, 3224, 45595, 20498, 16472, 13412, 16606, 21984, 12602, 5016, 8717, 44510, 10721, 3714, 5016, 46958, 25720, 10874, 16254, 41891, 45030, 1863, 2407, 7649, 3390, 6677, 3714, 4117, 2407, 8315, 2655, 32748, 2304, 20666, 3555, 28259, 10632, 19446, 31439, 37279, 16572, 5172, 32748, 2304, 22807, 19446, 31439, 2423, 3390, 22807, 46811, 10943, 2423, 3390, 34051, 32748, 2304, 31439, 6225, 3555, 9640, 3660, 18871, 21136, 16373, 1211, 16247, 5016, 29973, 264, 2182, 9113, 300, 32685, 12214, 484, 295, 428, 1772, 562, 291, 362, 23389, 257, 1723, 420, 2452, 10454], "avg_logprob": -0.09030330911570904, "compression_ratio": 1.596958174904943, "no_speech_prob": 6.377696990966797e-06, "words": [{"start": 4.39, "end": 4.87, "word": "السلام", "probability": 0.8875325520833334}, {"start": 4.87, "end": 5.29, "word": " عليكم", "probability": 0.95458984375}, {"start": 5.29, "end": 5.69, "word": " ورحمة", "probability": 0.855224609375}, {"start": 5.69, "end": 5.85, "word": " الله", "probability": 0.970703125}, {"start": 5.85, "end": 6.49, "word": " وبركاته", "probability": 0.99150390625}, {"start": 6.49, "end": 6.79, "word": " اليوم", "probability": 0.843505859375}, {"start": 6.79, "end": 6.95, "word": " ان", "probability": 0.71142578125}, {"start": 6.95, "end": 7.11, "word": " شاء", "probability": 0.98583984375}, {"start": 7.11, "end": 7.31, "word": " الله", "probability": 0.94091796875}, {"start": 7.31, "end": 7.55, "word": " رح", "probability": 0.887939453125}, {"start": 7.55, "end": 7.97, "word": " نبدأ", "probability": 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{"start": 603.89, "end": 604.95, "word": " تشغل", "probability": 0.9864501953125}, {"start": 604.95, "end": 605.31, "word": " حيث", "probability": 0.9187825520833334}, {"start": 605.31, "end": 605.75, "word": " 55", "probability": 0.8271484375}, {"start": 605.75, "end": 606.31, "word": "%", "probability": 0.9658203125}, {"start": 606.31, "end": 606.73, "word": " من", "probability": 0.98876953125}, {"start": 606.73, "end": 607.19, "word": " الدم", "probability": 0.9873046875}, {"start": 607.19, "end": 607.89, "word": " و", "probability": 0.95751953125}, {"start": 607.89, "end": 609.61, "word": " طبعا", "probability": 0.9808349609375}, {"start": 609.61, "end": 609.91, "word": " هي", "probability": 0.8720703125}, {"start": 609.91, "end": 610.43, "word": " عبارة", "probability": 0.9976806640625}, {"start": 610.43, "end": 610.59, "word": " عن", "probability": 0.998046875}, {"start": 610.59, "end": 610.71, "word": " ال", "probability": 0.62158203125}, {"start": 610.71, "end": 611.31, "word": 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نسبة البلازمة بالنسبة لهذه العينة هي نفسها نسبة البلازمة بالنسبة للدم كله اللي موجود داخل جسم الإنسان تمام؟ طيب", "tokens": [2407, 25961, 2655, 29538, 18863, 9957, 3660, 3714, 2304, 12984, 37977, 34051, 20449, 3555, 25720, 18871, 32748, 2304, 6156, 1211, 15042, 5551, 7435, 1829, 5551, 5172, 36520, 11296, 46811, 10943, 12602, 39319, 5551, 5172, 36520, 8717, 35457, 3660, 29739, 1211, 31377, 46007, 20666, 1863, 35457, 3660, 46740, 24192, 18863, 9957, 3660, 39896, 8717, 36178, 11296, 8717, 35457, 3660, 29739, 1211, 31377, 46007, 20666, 1863, 35457, 3660, 24976, 40448, 28242, 3224, 13672, 1829, 3714, 29245, 23328, 11778, 47283, 1211, 10874, 38251, 33688, 1863, 3794, 7649, 46811, 10943, 22807, 23032, 1829, 3555], "avg_logprob": -0.13452953853449978, "compression_ratio": 1.9585798816568047, "no_speech_prob": 1.329183578491211e-05, "words": [{"start": 651.59, "end": 651.73, "word": "و", "probability": 0.98193359375}, {"start": 651.73, "end": 652.21, "word": " كانت", "probability": 0.734375}, 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oxygen اللي موجود في جسمنا موجودة على بروتينات ال myoglobin اللي موجودة في العضلات و ال cytochrome اللي موجودة في .. في ..", "tokens": [2655, 1211, 9307, 9957, 8978, 9673, 10632, 4386, 2597, 6055, 1211, 9307, 3660, 4386, 6055, 1211, 9307, 3660, 8978, 9673, 10632, 9154, 2423, 9169, 13672, 1829, 3714, 29245, 23328, 8978, 10874, 38251, 8315, 3714, 29245, 23328, 3660, 15844, 4724, 32887, 2655, 9957, 9307, 2423, 452, 45841, 13496, 13672, 1829, 3714, 29245, 23328, 3660, 8978, 18863, 11242, 1211, 9307, 4032, 2423, 3185, 1353, 339, 11505, 13672, 1829, 3714, 29245, 23328, 3660, 8978, 4386, 8978, 4386], "avg_logprob": -0.17187499403953552, "compression_ratio": 1.88, "no_speech_prob": 1.2576580047607422e-05, "words": [{"start": 1291.46, "end": 1292.04, "word": "تلاتين", "probability": 0.758148193359375}, {"start": 1292.04, "end": 1292.22, "word": " في", "probability": 0.78857421875}, {"start": 1292.22, "end": 1292.74, "word": " المية", "probability": 0.859130859375}, {"start": 1292.74, "end": 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هنا عندنا ال .. الخلايا المحببة ال .. ال neutrophil و ال eosinophil و ال basophil شايفين هم خلايا محببة، هي هنا في شكل أوضح عندى هنا ال neutrophil", "tokens": [39237, 10943, 22807, 8717, 1829, 7435, 1829, 8717, 8592, 38688, 34105, 12399, 8032, 47302, 34105, 43242, 8315, 2423, 4386, 33962, 15040, 25528, 9673, 5016, 3555, 49401, 2423, 4386, 2423, 7989, 11741, 388, 4032, 2423, 308, 329, 259, 5317, 388, 4032, 2423, 987, 5317, 388, 13412, 995, 33911, 9957, 8032, 2304, 16490, 15040, 25528, 3714, 5016, 3555, 49401, 12399, 39896, 34105, 8978, 13412, 28820, 34051, 11242, 5016, 43242, 7578, 34105, 2423, 7989, 11741, 388], "avg_logprob": -0.25084460788481944, "compression_ratio": 1.7337662337662338, "no_speech_prob": 1.2516975402832031e-06, "words": [{"start": 1736.42, "end": 1737.36, "word": "تمام؟", "probability": 0.7044270833333334}, {"start": 1737.36, "end": 1737.66, "word": " نيجي", "probability": 0.859619140625}, {"start": 1737.66, "end": 1738.14, "word": " نشوف", "probability": 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"كلمة coagulation لحالها هذه coagulants يعني تجلط، تمام؟ anticoagulants يعني عوامل ماني على التجلط", "tokens": [4117, 19528, 3660, 598, 559, 2776, 5296, 5016, 6027, 11296, 29538, 598, 559, 425, 1719, 37495, 22653, 6055, 7435, 1211, 9566, 12399, 46811, 10943, 22807, 2511, 2789, 559, 425, 1719, 37495, 22653, 6225, 2407, 10943, 1211, 3714, 7649, 1829, 15844, 16712, 7435, 1211, 9566], "avg_logprob": -0.17934027247958714, "compression_ratio": 1.3773584905660377, "no_speech_prob": 2.384185791015625e-07, "words": [{"start": 2831.47, "end": 2831.93, "word": "كلمة", "probability": 0.95654296875}, {"start": 2831.93, "end": 2832.87, "word": " coagulation", "probability": 0.9386393229166666}, {"start": 2832.87, "end": 2833.69, "word": " لحالها", "probability": 0.912353515625}, {"start": 2833.69, "end": 2833.89, "word": " هذه", "probability": 0.223388671875}, {"start": 2833.89, "end": 2834.69, "word": " coagulants", "probability": 0.83258056640625}, {"start": 2834.69, "end": 2835.11, "word": " 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فبالتالي إما بتفصللي plasma أو بتفصللي serum لما أحطها على جهاز ال centrifuge فبالتالي حسب إيش مكوناتها أو إيش محتويات ال tube", "tokens": [3615, 3555, 9640, 3660, 4724, 25708, 1211, 20292, 36145, 3714, 33753, 45865, 9307, 3224, 3714, 46456, 46538, 3660, 46811, 10943, 8978, 2423, 2511, 2789, 559, 425, 394, 13672, 1829, 3714, 29245, 23328, 8978, 3224, 3714, 46456, 46538, 11933, 15042, 4724, 1829, 30544, 8978, 11296, 2511, 2789, 559, 425, 394, 3714, 46456, 46538, 34051, 4724, 1829, 30544, 8592, 8978, 11296, 2511, 2789, 559, 425, 394, 16490, 6027, 9381, 30767, 1829, 29739, 1211, 7649, 8032, 47302, 21542, 2304, 25720, 46811, 10943, 6156, 3555, 6027, 2655, 6027, 1829, 11933, 15042, 39894, 5172, 36520, 20292, 22564, 34051, 39894, 5172, 36520, 20292, 32755, 5296, 15042, 5551, 5016, 9566, 11296, 15844, 10874, 3224, 31377, 2423, 44828, 7181, 6156, 3555, 6027, 2655, 6027, 1829, 11331, 35457, 11933, 1829, 8592, 3714, 4117, 2407, 8315, 2655, 11296, 34051, 11933, 1829, 8592, 3714, 33753, 45865, 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"probability": 0.9806640625}, {"start": 3293.99, "end": 3294.47, "word": " خالص", "probability": 0.9983723958333334}, {"start": 3294.47, "end": 3294.73, "word": " زي", "probability": 0.959228515625}, {"start": 3294.73, "end": 3295.21, "word": " البلان", "probability": 0.5915120442708334}, {"start": 3295.21, "end": 3295.57, "word": " هاي", "probability": 0.788330078125}, {"start": 3295.57, "end": 3296.13, "word": " الحمرة", "probability": 0.736572265625}, {"start": 3296.13, "end": 3297.03, "word": " تمام", "probability": 0.956787109375}, {"start": 3297.03, "end": 3298.09, "word": " فبالتالي", "probability": 0.9353841145833334}, {"start": 3298.09, "end": 3298.27, "word": " إما", "probability": 0.935302734375}, {"start": 3298.27, "end": 3298.85, "word": " بتفصللي", "probability": 0.84478759765625}, {"start": 3298.85, "end": 3299.29, "word": " plasma", "probability": 0.81689453125}, {"start": 3299.29, "end": 3299.51, "word": " أو", "probability": 0.93310546875}, {"start": 3299.51, "end": 3300.05, "word": " بتفصللي", "probability": 0.965576171875}, {"start": 3300.05, "end": 3300.45, "word": " serum", "probability": 0.91650390625}, {"start": 3300.45, "end": 3301.15, "word": " لما", "probability": 0.952880859375}, {"start": 3301.15, "end": 3302.01, "word": " أحطها", "probability": 0.89990234375}, {"start": 3302.01, "end": 3302.17, "word": " على", "probability": 0.8671875}, {"start": 3302.17, "end": 3302.49, "word": " جهاز", "probability": 0.9957682291666666}, {"start": 3302.49, "end": 3302.63, "word": " ال", "probability": 0.97412109375}, {"start": 3302.63, "end": 3303.33, "word": " centrifuge", "probability": 0.954833984375}, {"start": 3303.33, "end": 3304.73, "word": " فبالتالي", "probability": 0.9901529947916666}, {"start": 3304.73, "end": 3305.57, "word": " حسب", "probability": 0.991943359375}, {"start": 3305.57, "end": 3306.17, "word": " إيش", "probability": 0.8141276041666666}, {"start": 3306.17, "end": 3306.85, "word": " مكوناتها", "probability": 0.8671061197916666}, {"start": 3306.85, "end": 3307.01, "word": " أو", "probability": 0.92626953125}, {"start": 3307.01, "end": 3307.13, "word": " إيش", "probability": 0.9720052083333334}, {"start": 3307.13, "end": 3307.67, "word": " محتويات", "probability": 0.974853515625}, {"start": 3307.67, "end": 3307.79, "word": " ال", "probability": 0.578125}, {"start": 3307.79, "end": 3308.05, "word": " tube", "probability": 0.912109375}], "temperature": 1.0}, {"id": 148, "seek": 331631, "start": 3308.49, "end": 3316.31, "text": "تمام؟ يا بنات كمية ال anticoagulant اللي موجودة أحيانا بتكون يعني لا تكتت الكارو", "tokens": [39237, 10943, 22807, 35186, 4724, 8315, 2655, 9122, 2304, 10632, 2423, 2511, 2789, 559, 425, 394, 13672, 1829, 3714, 29245, 23328, 3660, 5551, 5016, 1829, 7649, 995, 39894, 30544, 37495, 22653, 20193, 6055, 4117, 2655, 2655, 33251, 9640, 2407], "avg_logprob": -0.18212891332805156, "compression_ratio": 1.2407407407407407, "no_speech_prob": 5.960464477539063e-08, "words": [{"start": 3308.49, "end": 3309.41, "word": "تمام؟", "probability": 0.783203125}, {"start": 3309.41, "end": 3309.51, "word": " يا", "probability": 0.481201171875}, {"start": 3309.51, "end": 3309.79, "word": " بنات", "probability": 0.9208984375}, {"start": 3309.79, "end": 3310.13, "word": " كمية", "probability": 0.9713541666666666}, {"start": 3310.13, "end": 3310.27, "word": " ال", "probability": 0.990234375}, {"start": 3310.27, "end": 3311.03, "word": " anticoagulant", "probability": 0.93134765625}, {"start": 3311.03, "end": 3311.23, "word": " اللي", "probability": 0.8515625}, {"start": 3311.23, "end": 3311.95, "word": " موجودة", "probability": 0.9864501953125}, {"start": 3311.95, "end": 3312.77, "word": " أحيانا", "probability": 0.95810546875}, {"start": 3312.77, "end": 3314.13, "word": " بتكون", "probability": 0.946044921875}, {"start": 3314.13, "end": 3314.95, "word": " يعني", "probability": 0.96044921875}, {"start": 3314.95, "end": 3315.33, "word": " لا", "probability": 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"end": 3322.32, "word": " التيوب", "probability": 0.609619140625}, {"start": 3322.32, "end": 3322.76, "word": " و", "probability": 0.9404296875}, {"start": 3322.76, "end": 3323.04, "word": " ممكن", "probability": 0.890869140625}, {"start": 3323.04, "end": 3323.18, "word": " ما", "probability": 0.7138671875}, {"start": 3323.18, "end": 3324.32, "word": " تشوفيها،", "probability": 0.8797607421875}, {"start": 3324.32, "end": 3324.4, "word": " ما", "probability": 0.84375}, {"start": 3324.4, "end": 3325.28, "word": " تلاحظيها،", "probability": 0.8941824776785714}, {"start": 3325.28, "end": 3325.46, "word": " لكن", "probability": 0.98046875}, {"start": 3325.46, "end": 3325.64, "word": " هي", "probability": 0.87744140625}, {"start": 3325.64, "end": 3325.96, "word": " بيكون", "probability": 0.9728190104166666}, {"start": 3325.96, "end": 3326.32, "word": " موجودة", "probability": 0.84075927734375}, {"start": 3326.32, "end": 3326.54, "word": " فيها", "probability": 0.984619140625}, {"start": 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{"start": 3333.06, "end": 3333.58, "word": " أسحب", "probability": 0.95361328125}, {"start": 3333.58, "end": 3333.94, "word": " الدم", "probability": 0.965087890625}, {"start": 3333.94, "end": 3334.3, "word": " لعند", "probability": 0.9235026041666666}, {"start": 3334.3, "end": 3334.7, "word": " علامة", "probability": 0.9930013020833334}, {"start": 3334.7, "end": 3335.16, "word": " معينة", "probability": 0.9892578125}, {"start": 3335.16, "end": 3335.28, "word": " في", "probability": 0.91162109375}, {"start": 3335.28, "end": 3335.68, "word": " التيوب", "probability": 0.81591796875}, {"start": 3335.68, "end": 3335.94, "word": " هذه،", "probability": 0.45263671875}, {"start": 3335.94, "end": 3336.04, "word": " يعني", "probability": 0.982421875}, {"start": 3336.04, "end": 3336.26, "word": " أنا", "probability": 0.658203125}, {"start": 3336.26, "end": 3336.38, "word": " لو", "probability": 0.229736328125}, {"start": 3336.38, "end": 3336.56, "word": " بدي", "probability": 0.7022705078125}, 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"word": " to", "probability": 0.9677734375}, {"start": 3668.68, "end": 3668.86, "word": " the", "probability": 0.88134765625}, {"start": 3668.86, "end": 3669.26, "word": " blood", "probability": 0.943359375}, {"start": 3669.26, "end": 3669.68, "word": " in", "probability": 0.9306640625}, {"start": 3669.68, "end": 3670.28, "word": " soluble", "probability": 0.69677734375}, {"start": 3670.28, "end": 3670.72, "word": " complex", "probability": 0.783203125}, {"start": 3670.72, "end": 3671.2, "word": " طبعا", "probability": 0.972412109375}, {"start": 3671.2, "end": 3671.54, "word": " احنا", "probability": 0.8841145833333334}, {"start": 3671.54, "end": 3672.02, "word": " حكينا", "probability": 0.989501953125}, {"start": 3672.02, "end": 3672.52, "word": " انه", "probability": 0.583984375}, {"start": 3672.52, "end": 3673.08, "word": " هذا", "probability": 0.931640625}, {"start": 3673.08, "end": 3673.4, "word": " النوع", "probability": 0.991455078125}, {"start": 3673.4, "end": 3673.64, "word": " من", "probability": 0.99609375}, {"start": 3673.64, "end": 3673.78, "word": " ال", "probability": 0.97216796875}, {"start": 3673.78, "end": 3674.58, "word": " anticoagulant", "probability": 0.864501953125}, {"start": 3674.58, "end": 3674.84, "word": " زي", "probability": 0.974853515625}, {"start": 3674.84, "end": 3674.94, "word": " و", "probability": 0.96728515625}, {"start": 3674.94, "end": 3675.12, "word": " زي", "probability": 0.806884765625}, {"start": 3675.12, "end": 3675.28, "word": " ال", "probability": 0.943359375}, {"start": 3675.28, "end": 3675.66, "word": " Edeta", "probability": 0.3900146484375}, {"start": 3675.66, "end": 3676.5, "word": " بيمنع", "probability": 0.8709716796875}, {"start": 3676.5, "end": 3677.08, "word": " التخطر", "probability": 0.912109375}, {"start": 3677.08, "end": 3677.24, "word": " من", "probability": 0.9990234375}, {"start": 3677.24, "end": 3677.52, "word": " خلال", "probability": 0.9912109375}, {"start": 3677.52, "end": 3678.12, "word": " ارتباطه", "probability": 0.96318359375}, {"start": 3678.12, "end": 3678.3, "word": " بال", "probability": 0.98193359375}, {"start": 3678.3, "end": 3678.82, "word": " calcium", "probability": 0.281005859375}, {"start": 3678.82, "end": 3679.5, "word": " واحنا", "probability": 0.8377278645833334}, {"start": 3679.5, "end": 3679.94, "word": " حكينا", "probability": 0.99609375}, {"start": 3679.94, "end": 3680.28, "word": " انه", "probability": 0.6505126953125}, {"start": 3680.28, "end": 3680.38, "word": " ال", "probability": 0.91650390625}, {"start": 3680.38, "end": 3680.74, "word": " calcium", "probability": 0.99609375}, {"start": 3680.74, "end": 3681.42, "word": " ضروري", "probability": 0.9244384765625}], "temperature": 1.0}, {"id": 165, "seek": 369733, "start": 3681.67, "end": 3697.33, "text": "لتنشيط عوامل التخطر فإذا مافيش calcium معناها عوامل التخطر مش روحت اتنشط فمافيش كلوت فبالتالي الدم رح يظل سائل فإذا عشان هيك بسمي هذه المواد anticoagulant مضادات للتخثر موانع للتخثر", "tokens": 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" فبالتالي", "probability": 0.9641927083333334}, {"start": 3689.37, "end": 3689.65, "word": " الدم", "probability": 0.960693359375}, {"start": 3689.65, "end": 3689.83, "word": " رح", "probability": 0.84912109375}, {"start": 3689.83, "end": 3690.11, "word": " يظل", "probability": 0.91650390625}, {"start": 3690.11, "end": 3690.77, "word": " سائل", "probability": 0.96826171875}, {"start": 3690.77, "end": 3691.37, "word": " فإذا", "probability": 0.8551432291666666}, {"start": 3691.37, "end": 3691.75, "word": " عشان", "probability": 0.9231770833333334}, {"start": 3691.75, "end": 3691.97, "word": " هيك", "probability": 0.916259765625}, {"start": 3691.97, "end": 3692.45, "word": " بسمي", "probability": 0.9609375}, {"start": 3692.45, "end": 3692.65, "word": " هذه", "probability": 0.8134765625}, {"start": 3692.65, "end": 3693.29, "word": " المواد", "probability": 0.96484375}, {"start": 3693.29, "end": 3694.61, "word": " anticoagulant", "probability": 0.8279296875}, {"start": 3694.61, "end": 3695.31, "word": " مضادات", "probability": 0.88916015625}, {"start": 3695.31, "end": 3695.95, "word": " للتخثر", "probability": 0.81939697265625}, {"start": 3695.95, "end": 3696.67, "word": " موانع", "probability": 0.936767578125}, {"start": 3696.67, "end": 3697.33, "word": " للتخثر", "probability": 0.9539794921875}], "temperature": 1.0}, {"id": 166, "seek": 371572, "start": 3697.6, "end": 3715.72, "text": "تروح ترتبط بالكالسيوم و بتمنع التخثر طيب TND الهيبرين نوع تالت من ال anticoagulant اللي أكتر شيوعا في ال hematology lab ولكن طبعا انا ممكن استخدمها من 15 ل 30 unit per ml تركيزه", "tokens": [2655, 32887, 5016, 6055, 43500, 3555, 9566, 20666, 4117, 6027, 3794, 1829, 20498, 4032, 39894, 27842, 3615, 16712, 9778, 49115, 23032, 1829, 3555, 314, 13360, 2423, 3224, 1829, 26890, 9957, 8717, 45367, 6055, 6027, 2655, 9154, 2423, 2511, 2789, 559, 425, 394, 13672, 1829, 5551, 4117, 2655, 2288, 13412, 1829, 45367, 995, 8978, 2423, 8636, 267, 1793, 2715, 35525, 19452, 23032, 3555, 3615, 995, 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الهيبرين", "probability": 0.7978515625}, {"start": 3703.26, "end": 3703.7, "word": " نوع", "probability": 0.952880859375}, {"start": 3703.7, "end": 3704.18, "word": " تالت", "probability": 0.9542643229166666}, {"start": 3704.18, "end": 3704.46, "word": " من", "probability": 0.99267578125}, {"start": 3704.46, "end": 3705.08, "word": " ال", "probability": 0.97265625}, {"start": 3705.08, "end": 3706.0, "word": " anticoagulant", "probability": 0.86201171875}, {"start": 3706.0, "end": 3706.42, "word": " اللي", "probability": 0.79931640625}, {"start": 3706.42, "end": 3706.86, "word": " أكتر", "probability": 0.915771484375}, {"start": 3706.86, "end": 3707.36, "word": " شيوعا", "probability": 0.8609619140625}, {"start": 3707.36, "end": 3707.48, "word": " في", "probability": 0.79443359375}, {"start": 3707.48, "end": 3707.58, "word": " ال", "probability": 0.96728515625}, {"start": 3707.58, "end": 3708.14, "word": " hematology", "probability": 0.8592122395833334}, {"start": 3708.14, "end": 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"probability": 0.9072265625}, {"start": 3714.8, "end": 3715.72, "word": " تركيزه", "probability": 0.94248046875}], "temperature": 1.0}, {"id": 167, "seek": 374519, "start": 3716.73, "end": 3745.19, "text": "و طبعا ممكن أحيانا يسببلي clumping ل ال blatelet في ناس عندهم حساسية من ال heparin فبالتالي ممكن أنا إذا ضفتلهم heparin لعينتهم يصير فيه clumping يصير فيه تجمعات ل ال blatelet و ال white pieces فبالتالي هدول الناس أنا إذا لاحظت إنه استخدمت ال anticoagulant هذا ال heparin و روحت أعد خلايا ال blatelet و أعد ال white pieces و لاجتها جليلة", "tokens": [2407, 23032, 3555, 3615, 995, 3714, 43020, 5551, 5016, 1829, 7649, 995, 7251, 35457, 3555, 20292, 596, 35313, 5296, 2423, 42780, 15966, 8978, 8717, 32277, 43242, 16095, 11331, 3794, 32277, 10632, 9154, 2423, 415, 2181, 259, 6156, 3555, 6027, 2655, 6027, 1829, 3714, 43020, 41850, 11933, 15730, 48812, 5172, 2655, 1211, 16095, 415, 2181, 259, 5296, 3615, 9957, 2655, 16095, 7251, 9381, 13546, 8978, 3224, 596, 35313, 7251, 9381, 13546, 8978, 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"probability": 0.85107421875}, {"start": 4255.04, "end": 4255.78, "word": " استفسار", "probability": 0.9812825520833334}, {"start": 4255.78, "end": 4257.7, "word": " بعتوليا", "probability": 0.79962158203125}, {"start": 4257.7, "end": 4258.0, "word": " بقى", "probability": 0.8108723958333334}, {"start": 4258.0, "end": 4258.14, "word": " على", "probability": 0.91064453125}, {"start": 4258.14, "end": 4258.4, "word": " group", "probability": 0.85498046875}, {"start": 4258.4, "end": 4258.54, "word": " ال", "probability": 0.9853515625}, {"start": 4258.54, "end": 4258.98, "word": " whatsapp", "probability": 0.63330078125}, {"start": 4258.98, "end": 4259.84, "word": " بجاوبكم", "probability": 0.9443359375}, {"start": 4259.84, "end": 4260.32, "word": " من", "probability": 0.9912109375}, {"start": 4260.32, "end": 4260.66, "word": " خلال", "probability": 0.9954427083333334}, {"start": 4260.66, "end": 4261.18, "word": " تسجيل", "probability": 0.9959716796875}, {"start": 4261.18, "end": 4261.66, "word": " صوتي", "probability": 0.95361328125}, {"start": 4261.66, "end": 4262.06, "word": " أو", "probability": 0.82275390625}, {"start": 4262.06, "end": 4262.94, "word": " بنتناقش", "probability": 0.888232421875}, {"start": 4262.94, "end": 4263.4, "word": " فيه", "probability": 0.994140625}, {"start": 4263.4, "end": 4263.94, "word": " عبر", "probability": 0.9970703125}, {"start": 4263.94, "end": 4264.62, "word": " محاضرة", "probability": 0.926513671875}, {"start": 4264.62, "end": 4264.88, "word": " ال", "probability": 0.8876953125}, {"start": 4264.88, "end": 4265.2, "word": " zoom", "probability": 0.82568359375}, {"start": 4265.2, "end": 4265.7, "word": " اللي", "probability": 0.90673828125}, {"start": 4265.7, "end": 4266.26, "word": " موعدها", "probability": 0.8575439453125}, {"start": 4266.26, "end": 4266.6, "word": " يوم", "probability": 0.993408203125}, {"start": 4266.6, "end": 4267.16, "word": " الصبت", "probability": 0.88330078125}, {"start": 4267.16, "end": 4267.96, "word": " يعطيكم", "probability": 0.856689453125}, {"start": 4267.96, "end": 4268.68, "word": " العافية", "probability": 0.9832356770833334}, {"start": 4268.68, "end": 4269.8, "word": " وموفقين", "probability": 0.8693359375}, {"start": 4269.8, "end": 4270.2, "word": " بإذن", "probability": 0.977294921875}, {"start": 4270.2, "end": 4270.44, "word": " الله", "probability": 0.947265625}], "temperature": 1.0}], "language": "ar", "language_probability": 1.0, "duration": 4271.334, "duration_after_vad": 4243.089374999994}
\ No newline at end of file
diff --git a/PL9fwy3NUQKwY9jl8xw-bWkVZkWcGGyspd/mJoJF0VELyo_raw.srt b/PL9fwy3NUQKwY9jl8xw-bWkVZkWcGGyspd/mJoJF0VELyo_raw.srt
new file mode 100644
index 0000000000000000000000000000000000000000..794ba8251d8ff6c2b37a5771fdf1e014da5e92f2
--- /dev/null
+++ b/PL9fwy3NUQKwY9jl8xw-bWkVZkWcGGyspd/mJoJF0VELyo_raw.srt
@@ -0,0 +1,3616 @@
+1
+00:00:04,390 --> 00:00:07,310
+السلام عليكم ورحمة الله وبركاته اليوم ان شاء الله
+
+2
+00:00:07,310 --> 00:00:11,690
+رح نبدأ محاضرة جديدة بعنوان blood components
+
+3
+00:00:11,690 --> 00:00:16,710
+مكونات الدم بالبداية ما هو تعريف الدم؟ ما هو ال
+
+4
+00:00:16,710 --> 00:00:22,530
+blood؟ تمام ال blood أو الدم هو عبارة عن السائل
+
+5
+00:00:22,530 --> 00:00:27,990
+الأحمر the red fluid that oozes out of your body
+
+6
+00:00:27,990 --> 00:00:31,690
+when you have sustained a cut or deep injuryإذا
+
+7
+00:00:31,690 --> 00:00:40,210
+كان في جرح أو يعني خدش لل skin أو للأوعية الدموية
+
+8
+00:00:40,210 --> 00:00:46,690
+تمام بحيث أنه هذا الجرح أخذ وقت استغرق وقت قبل
+
+9
+00:00:46,690 --> 00:00:52,490
+التقامه فأكيد رح يخرج منه سائل أحمر هو ال blood
+
+10
+00:00:52,490 --> 00:00:56,860
+تمام و طبعا احنا اكيد يعني we are familiarwith
+
+11
+00:00:56,860 --> 00:01:00,480
+blood احنا بنعرف ال blood وشوفنا ال blood قبل هيك
+
+12
+00:01:00,480 --> 00:01:09,120
+و بنعرف يعني إيش مكوناته، تمام؟ طيب فالسائل الأحمر
+
+13
+00:01:09,120 --> 00:01:15,310
+هذا هو عبارة عن سائل أكتركتر كثافة من الماء و ايضا
+
+14
+00:01:15,310 --> 00:01:20,470
+تقريبا من تلت لاربع مرات اكتر viscosity اكتر لزوجة
+
+15
+00:01:20,470 --> 00:01:26,490
+من الماء ايضا blood volume حجم السائق حجم السائل
+
+16
+00:01:26,490 --> 00:01:31,450
+هذا الأحمر او الدم طبعا هو متغير variable يختلف من
+
+17
+00:01:31,450 --> 00:01:36,970
+شخص الى اخر ولكن هو غالبا بيكون تمانية في المية من
+
+18
+00:01:36,970 --> 00:01:42,110
+كتلة جسم الإنسان يعني احنا طبعا كتلة جسمنامتغيرة،
+
+19
+00:01:42,110 --> 00:01:47,110
+في ناس بيكونوا اطوال اسمان، في ناس بيكونوا نحاف،
+
+20
+00:01:47,110 --> 00:01:51,190
+اقصار، فكتلة جسمهم متغيرة، فبالتالي حجم الدم
+
+21
+00:01:51,190 --> 00:01:56,710
+متغير، ولكن المتعارف عليه ان هو بيحتل تمانية في
+
+22
+00:01:56,710 --> 00:01:59,590
+المية أو يعني بيكون .. بياخد تمانية في المية من
+
+23
+00:01:59,590 --> 00:02:04,870
+كتلة الجسممثلا لو كان شخص وزنه خمسين kilogram
+
+24
+00:02:04,870 --> 00:02:09,050
+معناها point zero تمانية في المية يعني تمانية من
+
+25
+00:02:09,050 --> 00:02:14,570
+مية، تمام؟ ضرب الخمسين بيكون حجم ال blood تبعه
+
+26
+00:02:14,570 --> 00:02:18,870
+أربعة liter الناس اللي .. اللي وزنها أكتر من خمسين
+
+27
+00:02:18,870 --> 00:02:22,330
+هيكون عندها blood volume أكتر و الناس اللي وزنها
+
+28
+00:02:22,330 --> 00:02:26,650
+أقل من خمسين أكيد رح يكون عندهم blood volume أقل،
+
+29
+00:02:26,650 --> 00:02:30,880
+تمام؟فإذا أعطيناكم وزن بالامتحان و حكيتلكم إنه
+
+30
+00:02:30,880 --> 00:02:34,520
+احسي بيه لقداش بيكون عنده blood volume بس ماعليك
+
+31
+00:02:34,520 --> 00:02:39,600
+إلا إنك أنت تدر ب 8 من 100 أو 8 على 100 في الوزن
+
+32
+00:02:39,600 --> 00:02:46,260
+تمام؟ طيب هناك عوامل أخرى تؤثر على الحجم الدم أو
+
+33
+00:02:46,260 --> 00:02:49,560
+ال blood volume ألا وهي ال body size احنا حكينا
+
+34
+00:02:49,560 --> 00:02:53,930
+يعني ال ..كتلة الجسم حجم الجسم بيأثر على حجم ال
+
+35
+00:02:53,930 --> 00:02:59,150
+blood amount of adipocyte كمان نسبة النسيج الدهني
+
+36
+00:02:59,150 --> 00:03:03,430
+في الجسم برضه كمان بتأثر على حجم ال blood و ال
+
+37
+00:03:03,430 --> 00:03:08,490
+electrolyte concentration جداش تركيز الأملح
+
+38
+00:03:08,490 --> 00:03:14,440
+والمعادن داخل الجسم أيضا بتأثر على حجم الدمولكن ال
+
+39
+00:03:14,440 --> 00:03:19,300
+.. ال .. الحجم اللي قدام يتراوح ما بين 4 لتر إلى 6
+
+40
+00:03:19,300 --> 00:03:23,480
+لتر و ال average volume هو 5 لتر يعني معظم الناس
+
+41
+00:03:23,480 --> 00:03:27,520
+بنلاحظ أنها هي في نص ال range واقعة من 4 على 6
+
+42
+00:03:27,520 --> 00:03:34,220
+المنتصف هو 5 لترطيب لو أنا جيت سحبت عينة دم من
+
+43
+00:03:34,220 --> 00:03:40,420
+وريد تمام وحفظيتها في انبوبة و هذه الانبوبة حطيتها
+
+44
+00:03:40,420 --> 00:03:46,140
+في جهاز اسمه centrifuge تمام جهاز الطرد المركزي
+
+45
+00:03:46,140 --> 00:03:52,440
+centrifuge تمام هذا الجهازوظيفته ان هو يفصل
+
+46
+00:03:52,440 --> 00:03:58,520
+لمكونات الدم يفصل مكونات الدم طبعا كيف بيفصلها؟ من
+
+47
+00:03:58,520 --> 00:04:04,300
+اسمه طرد مركزي يعني الأشياء الثقيلة او ال ..
+
+48
+00:04:04,300 --> 00:04:09,840
+الوزنها ثقيل بتنزل لتحت في قاع ال tube و الأشياء
+
+49
+00:04:09,840 --> 00:04:13,920
+الخفيفة او ال .. او ال .. المكونات اللي وزنها خفيف
+
+50
+00:04:13,920 --> 00:04:18,440
+بتكون موجودة في the top of the tube في أعلى ال
+
+51
+00:04:18,440 --> 00:04:21,950
+tubeزي هنا مثلا بعد ما أنا أطلعها من جهاز ال
+
+52
+00:04:21,950 --> 00:04:26,250
+centrifuge تمام بلاحظ أنه الضم فصل إلى هذه
+
+53
+00:04:26,250 --> 00:04:30,510
+المكونات جزء الخلايا اللي موجود في قاع ال tube
+
+54
+00:04:30,510 --> 00:04:35,950
+تمام و جزء السائل اللي موجود في at the top of the
+
+55
+00:04:35,950 --> 00:04:40,790
+tube طبعا الخلايا أثقل لذلك كانت موجودة في قاع ال
+
+56
+00:04:40,790 --> 00:04:45,350
+tube أما السائل فهو أخف فعشان هيك كان موجود في the
+
+57
+00:04:45,350 --> 00:04:48,770
+top of the tubeطيب خلينا نشوف إيش السائل إيش مكو
+
+58
+00:04:48,770 --> 00:04:54,270
+.. إيش اسمه السائل هذا المكون اسمه plasma تمام؟ و
+
+59
+00:04:54,270 --> 00:04:57,370
+المكونات
+
+60
+00:04:57,370 --> 00:05:03,070
+الخلايا في عندي طبقة بيضاء خفيفة جدا كخيط رفيع
+
+61
+00:05:03,070 --> 00:05:07,450
+بسميها ال buffy coat اللي بتكون مكونا أو مع ..
+
+62
+00:05:07,450 --> 00:05:12,850
+مكونا من خلاية دم البيضاء ال white bases و الصفائح
+
+63
+00:05:12,850 --> 00:05:20,240
+الدموية البليتلاتطبعا وفي عندى الطبقة حمراء اللون
+
+64
+00:05:20,240 --> 00:05:25,160
+بتكون موجودة في القاع بسميها الاريثروسايت او خلايا
+
+65
+00:05:25,160 --> 00:05:30,880
+دم الحمراء ال RBCs او ال red blood cell طبعا طيبكل
+
+66
+00:05:30,880 --> 00:05:34,760
+من الطبقة البيضاء و الطبقة الحمراء بسميها formed
+
+67
+00:05:34,760 --> 00:05:39,180
+element هذه هي مكونات الدم، هلأ راح نشوف إيش
+
+68
+00:05:39,180 --> 00:05:43,560
+مكوناته اللي .. اللي .. اللي يعني متحدثين عنها في
+
+69
+00:05:43,560 --> 00:05:49,020
+ال slides هلأ الدم إذا أنا فصلته طبعا راح يعطيني
+
+70
+00:05:49,020 --> 00:05:54,860
+three layers، تلات طبقاتبشوفهم لما أنا بعمل إيش
+
+71
+00:05:54,860 --> 00:05:59,180
+centrifugation لل blood لما أنا بدي يكون عندي دم
+
+72
+00:05:59,180 --> 00:06:04,140
+مفصول بال centrifuge بيكون يعني مفصول إلى تلت
+
+73
+00:06:04,140 --> 00:06:10,500
+طبقات الطبقة الأولى بسميها straw colored liquid أو
+
+74
+00:06:10,500 --> 00:06:16,220
+بسميها plasma هي سائلماخذ اللون القشي straw
+
+75
+00:06:16,220 --> 00:06:21,200
+colored liquid سائل ماخذ اللون القشي و بسميه ال
+
+76
+00:06:21,200 --> 00:06:26,700
+plasma موجود at the top لإنه خفيف تمام و بيحتل
+
+77
+00:06:26,700 --> 00:06:31,780
+تقريبا خمسة و خمسين في المية من حجم الدم في عندي
+
+78
+00:06:31,780 --> 00:06:37,000
+طبقة بسميها thin cream colored layer يعني طبقة
+
+79
+00:06:37,000 --> 00:06:43,550
+رفيعةو لونها كريمي لونها لون أبيض بسميها ال buffy
+
+80
+00:06:43,550 --> 00:06:49,090
+coat و هذه ال buffy coat هي عبارة عن إيش؟ ال white
+
+81
+00:06:49,090 --> 00:06:54,290
+blood cells خلايا الدم البيضاء and ال platelets
+
+82
+00:06:54,290 --> 00:07:00,430
+الصفائحتمام و هذه طبعا بيكون حجمها أقل من واحد في
+
+83
+00:07:00,430 --> 00:07:04,810
+المية تمام؟ أقل من واحد في المية أو تقريبا يعني
+
+84
+00:07:04,810 --> 00:07:09,250
+احنا بنقربها بنحكي واحد في المية الطبقة التالتة
+
+85
+00:07:09,250 --> 00:07:14,790
+اللي هي at the bottomفي قاع ال tube هي طبقة ال red
+
+86
+00:07:14,790 --> 00:07:20,210
+blood cells وهي عبارة عن heavy layer طبقة ثقيلة
+
+87
+00:07:20,210 --> 00:07:28,270
+وموجودة في البطم في قاع ال tube و تحتل حجم 45% من
+
+88
+00:07:28,270 --> 00:07:32,520
+ال whole blood من عينة الدم اللي أنا سحبتهاطبعا ال
+
+89
+00:07:32,520 --> 00:07:38,140
+cells هذه الخلايا بتكون عبارة عن معلقة في داخل
+
+90
+00:07:38,140 --> 00:07:43,820
+سائل طبعا معلقة داخل سائل فاللي هو ال plasma يعني
+
+91
+00:07:45,120 --> 00:07:48,880
+فإذا أنا ممكن أفصل مكونات الدم إما عن طريق ال
+
+92
+00:07:48,880 --> 00:07:53,300
+filtration باستخدام filter أو باستخدام ال
+
+93
+00:07:53,300 --> 00:07:56,980
+centrifugation اللي هو باستخدام جهاز لل centrifuge
+
+94
+00:07:56,980 --> 00:08:02,220
+أعمله spinning دوران أو التفاف لهذا الجهاز مجرد ما
+
+95
+00:08:02,220 --> 00:08:08,100
+أشغله فيه الكهربا بيصير يعمل دوران التفافات تمام
+
+96
+00:08:08,100 --> 00:08:13,240
+سريعة جدا وبالتالي على إثر هذه التفافات الأجزاء
+
+97
+00:08:13,240 --> 00:08:18,440
+الثقيلة منالسائل بتهبط للأسفل بتنزل at the bottom
+
+98
+00:08:18,440 --> 00:08:22,460
+of the tube و الأجزاء الخفيفة بتكون موجودة at the
+
+99
+00:08:22,460 --> 00:08:27,220
+top of the tube اذا لو انا اعتبرت انه العينة كلها
+
+100
+00:08:27,220 --> 00:08:31,020
+هاي اللي انا عبيتها في ال tube 100% تمام لو
+
+101
+00:08:31,020 --> 00:08:35,990
+اعتبرتها انها كلها 100%فال plasma هتحتل تقريبا
+
+102
+00:08:35,990 --> 00:08:39,310
+خمسة و خمسين في المية ال buffy coat يعني واحد في
+
+103
+00:08:39,310 --> 00:08:43,330
+المية او اقل و ال erythrocyte خمسة و اربعين في
+
+104
+00:08:43,330 --> 00:08:47,370
+المية فبالتالي يعني ان هو هم تقريبا كلهم بيكونوا
+
+105
+00:08:47,370 --> 00:08:51,230
+مية في المية ماشي حكينا ان ال buffy coat لونها
+
+106
+00:08:51,230 --> 00:08:56,660
+creamy و thin layerو مكونة من ال white BCs و
+
+107
+00:08:56,660 --> 00:09:00,740
+البلاتلت ال erythrocyte هي الطبقة الحمراء مكونة من
+
+108
+00:09:00,740 --> 00:09:06,360
+ال red blood cell و ال .. ال plasma هي ال .. ال
+
+109
+00:09:06,360 --> 00:09:10,860
+straw colored liquid السائل اللي لونه قش اللون
+
+110
+00:09:10,860 --> 00:09:14,200
+تمام؟ لونه أصفر طيب ال erythrocyte إيش يعني
+
+111
+00:09:14,200 --> 00:09:18,340
+erythrocyte؟ لو أنا جيت قطعتها erythro لحالها
+
+112
+00:09:18,340 --> 00:09:23,500
+erythro لحالها تمام؟ معناها red colorيعني red
+
+113
+00:09:23,500 --> 00:09:28,400
+color sites sites معناها خلايا، تمام؟ ف erythro
+
+114
+00:09:28,400 --> 00:09:33,120
+red و sites خلايا إذا red cells إذا أنا أحكي
+
+115
+00:09:33,120 --> 00:09:39,560
+erythrocyte أو أحكي red blood cells، تمام؟ أو RBCs
+
+116
+00:09:39,560 --> 00:09:44,520
+هي نفس المعنى، أنا بقصد خلايا، الدم الحمراء طيب
+
+117
+00:09:44,520 --> 00:09:51,970
+نيجي لـ plasma هو أولسائل يعني بيكون هو طبقة من
+
+118
+00:09:51,970 --> 00:09:55,670
+طبقات ال blood و بتكون موجودة at the bottom خلينا
+
+119
+00:09:55,670 --> 00:09:59,030
+نحكي عنها و نشوف إيش هي إيش هي ال plasma من إيش
+
+120
+00:09:59,030 --> 00:10:03,270
+مكوّنة طبعا حكينا إنه هي straw colored liquid و
+
+121
+00:10:03,270 --> 00:10:10,710
+تقريبا تشغل حيث 55% من الدم و طبعا هي عبارة عن ال
+
+122
+00:10:10,710 --> 00:10:16,390
+transport mediumهي الخلايا الدم بتكون معلقة في هذه
+
+123
+00:10:16,390 --> 00:10:20,770
+ال plasma إذا هي بتتكفل في نقل خلايا الدم من و إلى
+
+124
+00:10:20,770 --> 00:10:25,650
+الأعضاء الجسم، تمام؟ إذا بتنقل ل blood cells و
+
+125
+00:10:25,650 --> 00:10:31,970
+لplatelet من و إلى أعضاء جسم الإنسان، تمام؟ هذه هي
+
+126
+00:10:31,970 --> 00:10:37,690
+السائلوطبعا لما انا احكي ان ال plasma تحتل نسبة 55
+
+127
+00:10:37,690 --> 00:10:46,330
+% من الدم في العينة او 55% من الدم في الجسم فانا
+
+128
+00:10:46,330 --> 00:10:50,590
+المعلومة واحدة يعني لإن انا بما اني أخدت عينة من
+
+129
+00:10:50,590 --> 00:10:55,490
+الدمو كانت هذه العينة ممثلة أو معبرة عن الدم فلما
+
+130
+00:10:55,490 --> 00:11:00,710
+أجي أفصلها تمام راح أفصل نسبة البلازمة بالنسبة
+
+131
+00:11:00,710 --> 00:11:04,350
+لهذه العينة هي نفسها نسبة البلازمة بالنسبة للدم
+
+132
+00:11:04,350 --> 00:11:09,790
+كله اللي موجود داخل جسم الإنسان تمام؟ طيبلذا لما
+
+133
+00:11:09,790 --> 00:11:15,130
+أحكي 55% من ال blood أو من ال سان بالنفس المعنى
+
+134
+00:11:15,130 --> 00:11:20,810
+طيب و مكونات هاي ال plasma من إيش مكونات؟ احنا
+
+135
+00:11:20,810 --> 00:11:25,270
+حكينا إن هي سائل أصفر اللون ولكن ما هي مكوناته؟
+
+136
+00:11:25,270 --> 00:11:30,290
+أكثر من 90% من هذا السائل هو عبارة عن water just
+
+137
+00:11:30,290 --> 00:11:35,780
+إيش water ال plasma هيماء بالأساس، تمام؟ أكتر من
+
+138
+00:11:35,780 --> 00:11:43,360
+90% منه ماء ولكن الأقل من 10% اللي ضايقين هي عبارة
+
+139
+00:11:43,360 --> 00:11:50,920
+عن أيوناتأملاح تمام ايونات اكساديوم كلورايد و
+
+140
+00:11:50,920 --> 00:11:55,400
+بتاسيوم اي اشي .. اي ايونات او املاح موجودة داخل
+
+141
+00:11:55,400 --> 00:12:00,180
+الجسم بروتينات اللي زي ال albumin و ال globulin و
+
+142
+00:12:00,180 --> 00:12:05,900
+ال fibrinogen بروتينات اللي بتعمل تخ .. تخثر للدم
+
+143
+00:12:05,900 --> 00:12:09,940
+موجودة ايضا في البلازما الانزيمات اللي بتقوم
+
+144
+00:12:09,940 --> 00:12:13,980
+بالوظائف المختلفة موجودة داخل البلازما هي عبارة عن
+
+145
+00:12:13,980 --> 00:12:19,750
+بلازما بروتينو أيضًا dissolved gases أو dissolved
+
+146
+00:12:19,750 --> 00:12:25,930
+gases، تمام؟ غازات مذابة داخل الدم بتكون موجودة في
+
+147
+00:12:25,930 --> 00:12:29,730
+البلازمة زي ال oxygen و يتعني أكسيد الكربون و
+
+148
+00:12:29,730 --> 00:12:34,930
+أيضًا موجود عباء .. موجود ال nutrients يعني مغذيات
+
+149
+00:12:34,930 --> 00:12:39,890
+أو مصادر للطاقة زي ال glucose و الأحماض الأمينية و
+
+150
+00:12:39,890 --> 00:12:43,030
+الأحماض الدهنية هذه بتكون موجودة أيضًا داخل
+
+151
+00:12:43,030 --> 00:12:47,170
+البلازمةبالإضافة إلى ال circulating tissue
+
+152
+00:12:47,170 --> 00:12:50,850
+products نواتج اللي بينتجها أو اللي بيطلعها
+
+153
+00:12:50,850 --> 00:12:54,790
+الأعضاء يعني ال west طبع الأعضاء زي اليوريا
+
+154
+00:12:54,790 --> 00:12:58,330
+كرياتينين لكتات هذه كلها ناتجة من ال metabolism
+
+155
+00:12:58,330 --> 00:13:02,830
+للأعضاء أو من الوثائق لإن الأعضاء عملت function في
+
+156
+00:13:02,830 --> 00:13:07,250
+جسم الإنسانفكان ناتج ال .. ال function هذه أو ناتج
+
+157
+00:13:07,250 --> 00:13:12,670
+ال metabolism تبعها ب .. مركبات بدنا نتخلص منها،
+
+158
+00:13:12,670 --> 00:13:16,310
+بدها تطلع إما في ال urine أو في ال stool فبالتالي
+
+159
+00:13:16,310 --> 00:13:20,550
+بنتكون .. إلا تمر عبر الدمتمام؟ اذا ال tissue
+
+160
+00:13:20,550 --> 00:13:24,630
+products او ال tissue waste نفس الاشي زي اليوريا و
+
+161
+00:13:24,630 --> 00:13:28,850
+الكرياتينين و اللكتات circulating hormones ايضا
+
+162
+00:13:28,850 --> 00:13:33,590
+موجود في هذه البلازما هرمونات زي الانسولين و ال
+
+163
+00:13:33,590 --> 00:13:37,850
+adrenaline و ال growth hormone و الهرمونات اخرى
+
+164
+00:13:37,850 --> 00:13:43,210
+تمام؟ موجودة بتكون داخل البلازما اذا البلازما هي
+
+165
+00:13:43,210 --> 00:13:48,360
+عبارة عن السائل الأصفر اللون او القش اللوناللي
+
+166
+00:13:48,360 --> 00:13:53,600
+موجود في at the top of centrifuged blood تمام؟ و
+
+167
+00:13:53,600 --> 00:14:00,720
+بيشكل خمسة و خمسين في المية من حجم الدم مكوناته
+
+168
+00:14:00,720 --> 00:14:06,400
+water، ماء، تمام؟ solvent، ماء و solutes هذه كلها
+
+169
+00:14:06,400 --> 00:14:10,520
+solutes بسميها خلينا نوظف التعريفات اللي احنا
+
+170
+00:14:10,520 --> 00:14:15,360
+اتعلمناها المحاضرة الماضية أي سائل مكون من solutes
+
+171
+00:14:15,360 --> 00:14:20,340
+and solvent صح؟ تمامفالبلازمة هي عبارة عن solvent
+
+172
+00:14:20,340 --> 00:14:25,140
+اللي هو المذيب الماء أما الأشياء المذابة داخله
+
+173
+00:14:25,140 --> 00:14:29,180
+ممكن تكون أيونات، أملاح، بروتينات، غازات،
+
+174
+00:14:29,180 --> 00:14:33,080
+nutrients، موغذيات، tissue product أو تشيوست أو
+
+175
+00:14:33,080 --> 00:14:39,340
+حتى hormones بتكون موجودة طيبفبالتالي إذا ال water
+
+176
+00:14:39,340 --> 00:14:44,040
+هو عبارة عن important .. important solvent مذيب
+
+177
+00:14:44,040 --> 00:14:48,840
+مهم للايونات و ال .. ال solutes اللي بتكون موجودة
+
+178
+00:14:48,840 --> 00:14:52,280
+فيه، ال vitaminات، ال enzymes، ال hormones، ال
+
+179
+00:14:52,280 --> 00:14:55,720
+protein، ال waste، كلها بتكون مضعبة داخل هذه
+
+180
+00:14:55,720 --> 00:15:01,330
+البلازمةطيب، في مصطلح أخر احنا ب .. ب .. ب ..
+
+181
+00:15:01,330 --> 00:15:05,210
+هيتردد معناه و هنستخدمه كتير اللي هو ال serum،
+
+182
+00:15:05,210 --> 00:15:09,090
+تمام؟ ال serum هو عبارة عن إيش؟ هلأ لو أنا ال
+
+183
+00:15:09,090 --> 00:15:15,590
+blood هذا اللي فصلته غير متجلة، تمام؟ إذا كان غير
+
+184
+00:15:15,590 --> 00:15:20,360
+متجلةيعني عوامل التجلط أو بروتينات التجلط ما تم
+
+185
+00:15:20,360 --> 00:15:25,300
+استهلاكها فمعناها إن هذا السائل اللي .. اللي ..
+
+186
+00:15:25,300 --> 00:15:31,480
+اللي موجود في الطب بسميه plasma، تمام؟ بسميه
+
+187
+00:15:31,480 --> 00:15:36,370
+plasmaلكن إذا كان الدم متجلط، إيش يعني الدم متجلط؟
+
+188
+00:15:36,370 --> 00:15:40,170
+يعني أصبح عبارة عن كتلة، بطل سائل، خلايا الدم
+
+189
+00:15:40,170 --> 00:15:45,110
+تجمعت و الـplatelet و الصفائح الدموية تجمعت و أصبح
+
+190
+00:15:45,110 --> 00:15:48,910
+الدم عبارة عن كتلة أو إشي صلب، سُلِد، تمام؟ بطل
+
+191
+00:15:48,910 --> 00:15:55,690
+سائل، معناه السائل اللي فوق بينفصل، بسمي serum،
+
+192
+00:15:55,690 --> 00:16:00,380
+تمام؟ طيبفي حالة البلازمة بت .. ال .. ال .. طبعا
+
+193
+00:16:00,380 --> 00:16:05,020
+ال .. الدم يا بنات موجود فيه بروتينات بسميها عوامل
+
+194
+00:16:05,020 --> 00:16:10,400
+تجلط، هذه البروتينات بتتنشط لما الدم يتجلط و
+
+195
+00:16:10,400 --> 00:16:15,740
+بتستهلك، تمام؟فعشان هيك إذا كان الدم غير متجلط
+
+196
+00:16:15,740 --> 00:16:19,440
+بتكون بروتينات التخثر أو عوامل التخثر أو عوامل
+
+197
+00:16:19,440 --> 00:16:23,440
+التجلط غير مستهلكة يعني لساتها موجودة لإن الدم ما
+
+198
+00:16:23,440 --> 00:16:28,460
+اتجلط فبسميش السائل اللي فوق plasma يعني بيحتوي
+
+199
+00:16:28,460 --> 00:16:33,360
+على fibrinogen تمام؟بيحتوي على fibrinogen لإنه مش
+
+200
+00:16:33,360 --> 00:16:37,000
+مستهلك ال .. ال .. ال fibrinogen ما تم استهلاكه
+
+201
+00:16:37,000 --> 00:16:43,340
+طيب فإذا ال plasma هي عبارة عن سائل الموجود at the
+
+202
+00:16:43,340 --> 00:16:48,700
+top of قاش الدم اللي لساته ما اتجلطتمام؟ أو اللي
+
+203
+00:16:48,700 --> 00:16:53,660
+غير متجلط وموجود فيه fibrinogen أما ال serum فهو
+
+204
+00:16:53,660 --> 00:16:58,120
+السائل اللي موجود at the top of ash clotted blood
+
+205
+00:16:58,120 --> 00:17:04,480
+على سطح الدم المتجلط وطالما أن الدم اتجلط معناها
+
+206
+00:17:04,480 --> 00:17:08,940
+تم استهلاك عوامل التخثر تم استهلاك بروتينات التخثر
+
+207
+00:17:08,940 --> 00:17:13,580
+فبالتالي السائل اللي موجود at the top لا يحتوي على
+
+208
+00:17:13,580 --> 00:17:20,490
+fibrinogen فبالتالي ashهو عبارة عن serum طبعا؟ إذا
+
+209
+00:17:20,490 --> 00:17:26,030
+لو أنا حكيت plasma ناقص fibrinogen إيش هيكون؟
+
+210
+00:17:26,030 --> 00:17:31,150
+هيكون serum طبعا؟ plasma ناقص fibrinogen هيكون
+
+211
+00:17:31,150 --> 00:17:36,230
+عبارة عن serum لإنه حكينا إنه ال serum يعني معناها
+
+212
+00:17:36,230 --> 00:17:39,950
+إن الدم اتجلط يعني تم استهلاك البروتينات التخطر
+
+213
+00:17:39,950 --> 00:17:43,450
+يعني تم استهلاك ال fibrinogen يعني مش موجود فيها
+
+214
+00:17:43,450 --> 00:17:47,580
+fibrinogenطبعا، إذا إيش بيفرق البلازمة عن ال ..
+
+215
+00:17:47,580 --> 00:17:50,980
+السيرام؟ هي فقط في مك .. في .. في محتويات عوامل
+
+216
+00:17:50,980 --> 00:17:55,120
+التخثر، البلازمة لساتها عوامل التخثر موجودة و غير
+
+217
+00:17:55,120 --> 00:17:59,200
+مستهلكة، أما السيرام تم استهلاكها، البلازمة فيها
+
+218
+00:17:59,200 --> 00:18:03,800
+فيبرينوجين، السيرام مافيش فيه فيبرينوجين، هذا هو
+
+219
+00:18:03,800 --> 00:18:08,840
+الفرق ما بينهمتمام؟ إذا نيجي نشوف ال serum هو
+
+220
+00:18:08,840 --> 00:18:12,560
+عبارة إن ال plasma differs slightly from what is
+
+221
+00:18:12,560 --> 00:18:17,780
+termed serum ال plasma بتختلف قليلا عن ما يسمى بال
+
+222
+00:18:17,780 --> 00:18:23,210
+serum إذا الإختلاف بسيط فيهمالسيرام هو عبارة عن
+
+223
+00:18:23,210 --> 00:18:27,850
+إيش؟ generally taken to mean the fluid part of the
+
+224
+00:18:27,850 --> 00:18:31,670
+blood which remain after the blood has coagulated
+
+225
+00:18:31,670 --> 00:18:37,090
+أو clotted هو عبارة عن ال fluid السائل اللي يتبقى
+
+226
+00:18:37,090 --> 00:18:41,830
+بعد إيش أو ينفصل at the top بعد إيش after the
+
+227
+00:18:41,830 --> 00:18:46,290
+blood has coagulated يعني clottedبعد ما الدم يصير
+
+228
+00:18:46,290 --> 00:18:50,850
+له coagulation يصير له clotted فبالتالي وينفصله
+
+229
+00:18:50,850 --> 00:18:55,690
+فالسائل الموجود at the top بسميه serum وإيش
+
+230
+00:18:55,690 --> 00:18:59,390
+معناها؟ مدام صار الدم .. لو الدم صار متخفر صار
+
+231
+00:18:59,390 --> 00:19:05,840
+متجلط تمام تحت في at the bottomمعناها تم استهلاك
+
+232
+00:19:05,840 --> 00:19:11,280
+بروتينات التخطر معناها مافيش فيه fibrinogen هذا
+
+233
+00:19:11,280 --> 00:19:15,480
+السائل القش اللون فبقى اسمه plasma وصار اسمه serum
+
+234
+00:19:18,070 --> 00:19:24,690
+طيب المكون الثاني من مكونات الدم هي ال red blood
+
+235
+00:19:24,690 --> 00:19:28,890
+cell تمام؟ هي الطبقة الحمراء اللي موجودة at the
+
+236
+00:19:28,890 --> 00:19:32,590
+bottom red blood cell هلأ ال red blood cell يا
+
+237
+00:19:32,590 --> 00:19:36,770
+بنات لو أنا جيت خلاية دم الحمراء ال erythrocyte
+
+238
+00:19:36,770 --> 00:19:43,320
+يعني تمام؟ لو أنا جيتطلعت عليها نظرة من .. يعني لو
+
+239
+00:19:43,320 --> 00:19:46,620
+أنا حطيتها تحت ال microscope و طلعت عليها من ال ..
+
+240
+00:19:46,620 --> 00:19:50,440
+من أعلى بشوفها عبارة عن discotheque قرصية زي شكل
+
+241
+00:19:50,440 --> 00:19:57,340
+ال CD مدورة تمام؟ و إذا كان أنا جيت و طلعت عليها
+
+242
+00:19:57,340 --> 00:20:02,280
+من الجانب من ال side view نظرة جانبية من جنبها
+
+243
+00:20:02,280 --> 00:20:06,860
+بشوفها بايكنكافشاب تمام؟ زي الساعة الرملية بيكون
+
+244
+00:20:06,860 --> 00:20:15,540
+شكلها بايكنكافشاب تمام؟طيب و تشغل 99% من خلايا
+
+245
+00:20:15,540 --> 00:20:21,220
+الدم لإنه عددها بالملايين خلايا ال RBCs طبعا أهم
+
+246
+00:20:21,220 --> 00:20:28,100
+إشي في ال RBCs أو أهم وظيفة لل RBCs إنها تحمل ال
+
+247
+00:20:28,100 --> 00:20:31,520
+red colored hemoglobin البروتين اللي اسمه
+
+248
+00:20:31,520 --> 00:20:36,860
+hemoglobin اللي لونه أحمر تمام؟عشان هيك هي اكتسبت
+
+249
+00:20:36,860 --> 00:20:41,480
+اللون الأحمر نسبة لوجود فيها .. لوجود أو لاحتواءها
+
+250
+00:20:41,480 --> 00:20:45,360
+على البروتين الأحمر في داخلها، طبعا؟ فعشان هيك
+
+251
+00:20:45,360 --> 00:20:49,940
+اسمها أخدت لونها أو .. واسمها من لون و البروتين
+
+252
+00:20:49,940 --> 00:20:56,120
+اللي موجود في داخلها و اللي بيشغل تقريبا 70% من
+
+253
+00:20:56,120 --> 00:21:02,170
+حجمهاطيب؟ و ال .. هذا ال homoglobin هو عبارة عن ال
+
+254
+00:21:02,170 --> 00:21:10,130
+protein موجود في حلقات iron أو iron .. يعني هو
+
+255
+00:21:10,130 --> 00:21:13,250
+عبارة عن iron containing ال protein، موجود فيه
+
+256
+00:21:13,250 --> 00:21:19,530
+حديد، تمام؟و هذا السبب لإن لونه أحمر وجود ذرات
+
+257
+00:21:19,530 --> 00:21:25,570
+الحديد فيه هي اللي بتكسبه اللون الأحمر طبعا هو هذا
+
+258
+00:21:25,570 --> 00:21:30,290
+البروتين بيحمل تقريبا 97% من ال oxygen اللي موجود
+
+259
+00:21:30,290 --> 00:21:34,980
+في جسمناتلاتين في المية .. sorry تلاتة .. تلاتة في
+
+260
+00:21:34,980 --> 00:21:39,440
+المية من ال oxygen اللي موجود في جسمنا موجودة على
+
+261
+00:21:39,440 --> 00:21:42,960
+بروتينات ال myoglobin اللي موجودة في العضلات و ال
+
+262
+00:21:42,960 --> 00:21:49,340
+cytochrome اللي موجودة في .. في ..في الخلايا
+
+263
+00:21:49,340 --> 00:21:54,280
+للتنفس، يعني للـ Oxygen أو للـ Energy Production،
+
+264
+00:21:54,280 --> 00:21:58,820
+لإنتاج الطاقة، هتاخدوا في الحياوية دورة Eclipse،
+
+265
+00:21:58,820 --> 00:22:03,880
+وهتاخدوا الانزيمات اللي اسمة الـ Cytochrome،
+
+266
+00:22:03,880 --> 00:22:08,480
+وهتشوفوا أن هي لازم أنها تعطي Electron للـ Oxygen
+
+267
+00:22:08,480 --> 00:22:12,280
+عشان يصير فيه طاقة، إذن هي تحتوي على Oxygen3% من
+
+268
+00:22:12,280 --> 00:22:16,000
+الأكسجين موجود على الmyoglobin و الcytochrome أما
+
+269
+00:22:16,000 --> 00:22:20,400
+97% من الأكسجين الموجود في جسم نوين موجود محمّذ
+
+270
+00:22:20,400 --> 00:22:24,100
+على ال homoglobin و ال homoglobin هو عبارة عن إيش؟
+
+271
+00:22:24,100 --> 00:22:28,380
+هو عبارة عن protein زائد iron ال iron أكسب ال
+
+272
+00:22:28,380 --> 00:22:32,140
+homoglobin اللون الأحمر عشان هيك الخلايا اكتسبت
+
+273
+00:22:32,140 --> 00:22:36,700
+اللون الأحمر و سرنا نسميها ال red blood cell أو ال
+
+274
+00:22:36,700 --> 00:22:47,630
+erythrocyte أو أحيانا أنا باختصرها بحكيلكمRBCs رد
+
+275
+00:22:47,630 --> 00:22:49,990
+بلد سلز
+
+276
+00:22:56,310 --> 00:23:03,350
+طيب ماشي، إذا الخلايا الدم الحمراء وظيفتها ash حمل
+
+277
+00:23:03,350 --> 00:23:07,310
+ال homoglobin يعني oxygen delivery، هي عبارة عن
+
+278
+00:23:07,310 --> 00:23:12,370
+delivery للأكسجين، بتوصل الأكسجين إلى ash around
+
+279
+00:23:12,370 --> 00:23:18,030
+the body، ده في كل .. في كافة مناطق الجسمو ممكن
+
+280
+00:23:18,030 --> 00:23:23,110
+إنها تحمل ثاني أكسيد الكربون بتحمل أو تعود محملة
+
+281
+00:23:23,110 --> 00:23:26,590
+بثاني أكسيد الكربون من ال .. من أنسجة الجسم و من
+
+282
+00:23:26,590 --> 00:23:29,910
+أعضاء الجسم بكمية قليلة طبعا من ثاني أكسيد الكربون
+
+283
+00:23:29,910 --> 00:23:34,930
+و بتم التخلص منها من الرئاتين عن طريق عملية الزفير
+
+284
+00:23:34,930 --> 00:23:42,250
+تمام؟ و بالتالي ..بس احنا بنتخلص من الكم .. من
+
+285
+00:23:42,250 --> 00:23:45,530
+كمية الغاز اللى ما بدناياها من ثانو اكسيد الكربون
+
+286
+00:23:45,530 --> 00:23:49,110
+عن طريق عملية الزفير جزء من ثانو اكسيد الكربون
+
+287
+00:23:49,110 --> 00:23:53,130
+بيكون موجود فى البلازمة لع .. حتى يكونلى blood
+
+288
+00:23:53,130 --> 00:24:00,210
+buffer تمام؟عهقت كربونات و ال carbonic acid
+
+289
+00:24:00,210 --> 00:24:04,270
+فبالتالي بيكون يعطينا blood buffer و احنا عرفنا
+
+290
+00:24:04,270 --> 00:24:07,590
+إيش يعني blood buffer و عرفنا من إيش مكوناته،
+
+291
+00:24:07,590 --> 00:24:11,930
+قولنا weak acid و ال base طبعته أو ال salt طبعه
+
+292
+00:24:11,930 --> 00:24:18,140
+carbonic acid و carbonateطيب ال white blood cells
+
+293
+00:24:18,140 --> 00:24:23,860
+الطبقة التالتة اللي هي بسميها white blood cells ال
+
+294
+00:24:23,860 --> 00:24:28,200
+white blood طبعا يا بنات لها اسم تاني leukocytes
+
+295
+00:24:28,200 --> 00:24:31,660
+لو جيت كلمة ليكو .. ليكو معناها شفافة أو white
+
+296
+00:24:31,660 --> 00:24:36,760
+تمام وcytes يعني خلايا إذا هي خلايا الدم البيضاء
+
+297
+00:24:37,030 --> 00:24:40,970
+تمام؟ أو خلايا الدم اللي مايلها لون، هاي معناها
+
+298
+00:24:40,970 --> 00:24:47,270
+فبالتالي هي عبارة عن جزء مهم و أساسي من ال blood
+
+299
+00:24:47,270 --> 00:24:51,710
+اللي وظيفته defense against external organism
+
+300
+00:24:51,710 --> 00:24:58,010
+بيدافع أو بيحمي الجسم من الكائنات أو الأجسام
+
+301
+00:24:58,010 --> 00:25:03,670
+الغريبة اللي تعتبر foreign غريبة تعتبر pathogen
+
+302
+00:25:03,670 --> 00:25:09,860
+ممرضة لجسم الإنسانطيب why the blood cells خلاي
+
+303
+00:25:09,860 --> 00:25:13,360
+الدم البيضاء ممكن انا اعطيها لقب ال sanitary
+
+304
+00:25:13,360 --> 00:25:16,480
+engineer؟ اش يعني sanitary engineer؟ يعني مهندس
+
+305
+00:25:16,480 --> 00:25:22,000
+اللي مسئول عن عمليات التنظيف والتوظيف داخل الجسم،
+
+306
+00:25:22,000 --> 00:25:27,490
+cleaning upcleaning up what؟ بنظف إيش؟ بنظف الجسم
+
+307
+00:25:27,490 --> 00:25:34,750
+من خلايا ميتة من بقايا التيشو من الأجسام الغريبة
+
+308
+00:25:34,750 --> 00:25:38,770
+المكونات الغريبة اللي بتدخل جسم الإنسان بيروح
+
+309
+00:25:38,770 --> 00:25:43,430
+بيلتهمها وبيتخلص منها لحتى يحمي جسم الإنسان من ال
+
+310
+00:25:43,430 --> 00:25:48,370
+external organisms أو ال external objects يا إما
+
+311
+00:25:48,370 --> 00:25:53,600
+أجسام غريبة أو كائنات غريبةطبعا؟ طيب، في عندي خمس
+
+312
+00:25:53,600 --> 00:25:57,800
+أنواع من ال .. ال white pieces أو الـ leukocytes،
+
+313
+00:25:57,800 --> 00:26:02,180
+في عندي neutrophil و eosinophil و basophil، طبعا؟
+
+314
+00:26:02,180 --> 00:26:06,760
+و في عندي monocytes و lymphocytes، خمس أنواع طيب،
+
+315
+00:26:06,760 --> 00:26:10,900
+خلينا نشوف إيش هم هدولة خلايا الدم أو إيش أنواعهم،
+
+316
+00:26:10,900 --> 00:26:16,020
+نشوف هناعلى خلايا الدم طبعا خلايا الدم احنا كلنا
+
+317
+00:26:16,020 --> 00:26:20,820
+بنعرف ان كلها يتم إنتاجها من إيش؟ من نخاع العضم من
+
+318
+00:26:20,820 --> 00:26:24,680
+مادة النخاع اللي موجودة في داخل العضام بتنتج اللي
+
+319
+00:26:24,680 --> 00:26:29,380
+موجودة طبعا فيها خلايا stem cell خلايا جدعية و هذه
+
+320
+00:26:29,380 --> 00:26:35,000
+الخلايا بتتميز بتنمو و تتميز و تنقسم و حتى تعطيني
+
+321
+00:26:35,000 --> 00:26:38,480
+رد blood cell خلايا الدم الحمراءحكيتلكم لو انا
+
+322
+00:26:38,480 --> 00:26:43,300
+اطلعت عليها من ايش ال .. ال .. ال top view من ال
+
+323
+00:26:43,300 --> 00:26:47,180
+.. من .. من فوق لت .. يعني من فوق اطلع عليها نظرة
+
+324
+00:26:47,180 --> 00:26:50,480
+علوية بشوف .. بشوفها زي على هيئة ال CD قرصية
+
+325
+00:26:50,480 --> 00:26:55,280
+discotheque أما إذا كانت اطلعت عليها من الجانب
+
+326
+00:26:55,280 --> 00:26:58,780
+بشوفها bicon cave ship زي الساعة الرملية يعني
+
+327
+00:26:58,780 --> 00:27:03,960
+مقاعدة الوجهين تمام؟ أما ال white blood cells في
+
+328
+00:27:03,960 --> 00:27:10,460
+عندي أنواعBesophil و Neutrophil وفيه Monocytes و
+
+329
+00:27:10,460 --> 00:27:14,260
+Lymphocytes طيب هدول ال .. ال Azinophil و ال
+
+330
+00:27:14,260 --> 00:27:20,140
+Besophil و ال Neutrophil بسميها خلايا محببة، خلايا
+
+331
+00:27:20,140 --> 00:27:24,100
+محببة إيش يعني خلايا محببة؟ مش .. مش المحببة يعني
+
+332
+00:27:24,100 --> 00:27:28,820
+إنها هي ال favored يعني المفضلة، لأ خلايا محببة
+
+333
+00:27:28,820 --> 00:27:33,310
+يعني إنه موجود في ال cytoplasm تبعهاحبيبات إذا
+
+334
+00:27:33,310 --> 00:27:37,290
+لاحظته هنا موجود في الـcytoplasm تبعها حبيبات عشان
+
+335
+00:27:37,290 --> 00:27:42,350
+هيك بسميها خلايا محببة أما الخلايا الغير محببة
+
+336
+00:27:42,350 --> 00:27:46,090
+يعني إن الـcytoplasm تبعها مش موجود في حبيبات أو
+
+337
+00:27:46,090 --> 00:27:50,190
+لا يورى فيها حبيبات تحت المايكروسكوب فبسميها
+
+338
+00:27:50,190 --> 00:27:54,870
+monocyte و lymphocyte تمام؟ إذا اللي بتنتهي ب site
+
+339
+00:27:54,870 --> 00:27:59,700
+lymphocyte و monocyte هي خلايا غير محببةأما إذا
+
+340
+00:27:59,700 --> 00:28:03,360
+كانت بتنتهي بكلمة fil, xenofil, bezofil,
+
+341
+00:28:03,440 --> 00:28:09,540
+neutrophil فهي خلايا مش محددة و لا ننسى أيضا انه
+
+342
+00:28:09,540 --> 00:28:16,460
+من هذا النخاع في عندي خلايا بتنقسم و بتعطيني ال
+
+343
+00:28:16,460 --> 00:28:22,010
+platelets الصفائح الدمويةتمام؟ خلينا نشوف هى هنا
+
+344
+00:28:22,010 --> 00:28:27,790
+agranulocyte agranul يعني حبيبات site خلايا يعني
+
+345
+00:28:27,790 --> 00:28:33,690
+خلايا معها حبيبات خلايا محببة agranulocyte فإيش
+
+346
+00:28:33,690 --> 00:28:39,000
+موجود في الcytoplasm تبعها؟ موجود حبيباتتمام؟ و ال
+
+347
+00:28:39,000 --> 00:28:43,860
+a-granulocyte ال a لما بيجي في بداية الكلمة بيجي
+
+348
+00:28:43,860 --> 00:28:49,340
+للنفي تمام؟ a-granulocyte يعني خلايا غير محببة
+
+349
+00:28:49,340 --> 00:28:53,580
+يعني ال cytoplasm تبعها مش موجود في حبيبات فبسميها
+
+350
+00:28:53,580 --> 00:28:59,580
+monocyte و lymphocyteتمام؟ نيجي نشوف هنا، هاي هنا
+
+351
+00:28:59,580 --> 00:29:05,440
+عندنا ال .. الخلايا المحببة ال .. ال neutrophil و
+
+352
+00:29:05,440 --> 00:29:10,640
+ال eosinophil و ال basophil شايفين هم خلايا محببة،
+
+353
+00:29:10,640 --> 00:29:16,720
+هي هنا في شكل أوضح عندى هنا ال neutrophilشوف هاي
+
+354
+00:29:16,720 --> 00:29:21,500
+هاي هاي granulocyte اللي هي ال neutrophil ال a
+
+355
+00:29:21,500 --> 00:29:26,460
+-xenophil و ال basophil أما الخلايا ال a
+
+356
+00:29:26,460 --> 00:29:31,360
+-granulocyte يعني غير محببة هي ال monocyte و ال
+
+357
+00:29:31,360 --> 00:29:34,780
+lymphocyte ال cytoplasm تبعها خالي من الحبيبات
+
+358
+00:29:34,780 --> 00:29:39,020
+طبعا هذه الحبيبات اللي فيها مواديعني تقتل
+
+359
+00:29:39,020 --> 00:29:43,900
+الميكروبات، فبنسميها .. يعني الحبيبات هذه عبارة عن
+
+360
+00:29:43,900 --> 00:29:49,500
+دي فيها يعني العتاد و العدة لخلايا اللي هذه من
+
+361
+00:29:49,500 --> 00:29:56,830
+شأنها إنها داثع عن إيش؟ عن جسم الإنسان، تمام؟طيب
+
+362
+00:29:56,830 --> 00:30:02,250
+لو جيت انا هنا هيعندي عينة blood لو فصلتها راح
+
+363
+00:30:02,250 --> 00:30:05,330
+ألاحظ انه انا في عندي إذا الدم غير متجلة معناها
+
+364
+00:30:05,330 --> 00:30:07,970
+الساقل ده إيش اسمه plasma يعني بيحتوي على
+
+365
+00:30:07,970 --> 00:30:12,090
+fibrinogen ال fibrinogen من إيش مكوّن أو sorry ال
+
+366
+00:30:12,090 --> 00:30:16,790
+plasma هي من إيش مكوّنة مكوّنة من ماء بنسبة أكتر
+
+367
+00:30:16,790 --> 00:30:21,670
+من 90% فيها ماء و الباقي solutes تمام الماء هو ال
+
+368
+00:30:21,670 --> 00:30:26,030
+solvent و الباقي solutes إيش هيgases غازات oxygen
+
+369
+00:30:26,030 --> 00:30:33,190
+تعني أكسيد الكربون كربونات waste تمام الفضلات أو
+
+370
+00:30:33,190 --> 00:30:38,630
+ال products زي الكرياتينين واللاكتات واليوريا
+
+371
+00:30:38,630 --> 00:30:44,890
+nutrient تمام مغذيات وفيتامينات glucose amino
+
+372
+00:30:44,890 --> 00:30:50,960
+acids fatty acids بروتينات زي ال ..بروتينات زي
+
+373
+00:30:50,960 --> 00:30:54,660
+الانزيمات و البروتينات المختلفة و البروتينات
+
+374
+00:30:54,660 --> 00:30:58,200
+الناقلة ال albumin و ال globulin و ال fibrinogen و
+
+375
+00:30:58,200 --> 00:31:02,200
+عوامل التجلط هذه كلها بروتينات تمام؟ و أيضا
+
+376
+00:31:02,200 --> 00:31:06,780
+hormones زي ال growth hormone و ال insulin و ال
+
+377
+00:31:06,780 --> 00:31:09,420
+adrenaline و ال cortisone هذه كلها عبارة عن
+
+378
+00:31:09,420 --> 00:31:13,920
+هرمونات بتكون أيضا موجودةداخل من مكونات البلازما
+
+379
+00:31:13,920 --> 00:31:19,120
+تمام؟ فى عندى هنا الطبقة ال thin layer البيضاء اش
+
+380
+00:31:19,120 --> 00:31:22,960
+بسمى .. من اش مكونة؟ من ال white blood cell و
+
+381
+00:31:22,960 --> 00:31:27,300
+البلاتلة و بسميها puffy coat تمام؟ اللى هو الطبقة
+
+382
+00:31:27,300 --> 00:31:31,540
+ال .. او ال .. ال coat الأبيض تمام؟ ال red blood
+
+383
+00:31:31,540 --> 00:31:34,340
+cell هى الجزء الثقيل اللى موجود at the bottom
+
+384
+00:31:34,340 --> 00:31:38,360
+ويعرفنا اش يعني red blood cell هى خلاية دم الحمراء
+
+385
+00:31:38,360 --> 00:31:42,510
+اللى تنقل ال oxygen وظيفتها oxygendelivery تمام
+
+386
+00:31:42,510 --> 00:31:46,210
+وضيفتها oxygen .. oxygen delivery لو كان هذا الدم
+
+387
+00:31:46,210 --> 00:31:52,370
+متخثر معناه هنا في عندى clot خلايا متجلطة و السائل
+
+388
+00:31:52,370 --> 00:31:57,150
+هذا عبارة عن إيش؟ عبارة عن serum بسميه ببطل اسمه
+
+389
+00:31:57,150 --> 00:32:03,410
+plasma اسمه serumطبعا؟ طيب إذا كان هذا السائل اسمه
+
+390
+00:32:03,410 --> 00:32:07,890
+serum يعني الدم تحته متجلط يعني تم استهلاك
+
+391
+00:32:07,890 --> 00:32:12,490
+البروتينات التجلط عوامل التجلط طبعا؟ إذا مافيش
+
+392
+00:32:12,490 --> 00:32:15,830
+فيبرينوجين الفيبرينوجين استهلكناه في التخثر
+
+393
+00:32:15,830 --> 00:32:21,410
+فبالتالي صار هنا إيش؟ عبارة عن بلازمة ناقص
+
+394
+00:32:21,410 --> 00:32:25,600
+فيبرينوجين عشان هيك بسميه serumنفس المكونات فيه بس
+
+395
+00:32:25,600 --> 00:32:29,300
+اللهم ان عوامل التخثر مش موجودة فيه يعني مافيش فيه
+
+396
+00:32:29,300 --> 00:32:32,180
+fibrinogen مافيش فيه thrombin مافيش فيه البروتينات
+
+397
+00:32:32,180 --> 00:32:36,260
+التانية اللي مسئولة عن التخثر فبالتالي بيصير اسمه
+
+398
+00:32:36,260 --> 00:32:40,420
+serum ال plasma و ال serum يعني بيشبهوا بعض في
+
+399
+00:32:40,420 --> 00:32:46,660
+التركيب في اللون ولكن ال plasma تحتوي على عوامل
+
+400
+00:32:46,660 --> 00:32:50,720
+التخثر اللي ما تم استهلاكها لإن الدم تحتها غير
+
+401
+00:32:50,720 --> 00:32:56,550
+متجلط أما هنا ال serumلا يحتوي على عوامل التخثر،
+
+402
+00:32:56,550 --> 00:33:01,350
+يعني الدم متجلة، لو أنا .. لهنا في هذه الحالة، لو
+
+403
+00:33:01,350 --> 00:33:04,830
+أنا السائل كان plasma و الدم غير متجلة، مجرد ما
+
+404
+00:33:04,830 --> 00:33:09,810
+أعمل mix للتيوب، يعني أعملها .. أحركها، تمام؟
+
+405
+00:33:09,810 --> 00:33:14,570
+للتيوب، بيختلط ال .. ال .. الخلايا بالسائل و بيرجع
+
+406
+00:33:14,570 --> 00:33:19,880
+دم تاني، تمام؟ بيرجعيله نفس قوام الدملكن في حالة
+
+407
+00:33:19,880 --> 00:33:23,560
+ال serum هانا serum و هانا ايش؟ دم متجلط، هانا فيه
+
+408
+00:33:23,560 --> 00:33:29,320
+clot لو أنا خلطتهم في بعض، مستحيل يرجع لدم سائل،
+
+409
+00:33:29,320 --> 00:33:34,220
+تمام؟ أو ياخذ قوام الدم زي ما .. لما جمعته أو لما
+
+410
+00:33:34,220 --> 00:33:38,340
+سحبته من المريض، لأ، مستحيل لإن الدم تخثر، خلاص،
+
+411
+00:33:38,340 --> 00:33:42,780
+اتجلط طيب، هذا هو الفرق ما بين ال plasma و ال
+
+412
+00:33:42,780 --> 00:33:47,400
+serum طيب نيجي نشوف ايش كمان حكيلي عن طبقة ال
+
+413
+00:33:47,400 --> 00:33:53,210
+white piecesحكينا إنه مكوّنة من خمس أنواع من
+
+414
+00:33:53,210 --> 00:33:56,730
+الخلايا اللي هي ال .. ال neutrophil, ozinophil و
+
+415
+00:33:56,730 --> 00:34:00,230
+bezophil و حكينا عنهم هدولة granulocyte, monocyte
+
+416
+00:34:00,230 --> 00:34:03,970
+و lymphocyte هدولة agranulocyte بصير إننا بنعرف
+
+417
+00:34:03,970 --> 00:34:08,670
+إيش يعني granulocyte و إيش يعني agranulocyte و إيش
+
+418
+00:34:08,670 --> 00:34:14,390
+الفرق ما بينهم، كلهمالهم دور في ال defense against
+
+419
+00:34:14,390 --> 00:34:18,070
+external organism ولكن الآلية مختلفة يعني مثلا هذه
+
+420
+00:34:18,070 --> 00:34:23,550
+الخلايا المحببة تحتوي في حبيبتها على انزيمات
+
+421
+00:34:23,550 --> 00:34:28,390
+وجزيئات قاتلة لالميكروبات ال monocyte بتلتهم
+
+422
+00:34:28,390 --> 00:34:32,710
+التهام الميكروب ال lymphocytes بتقتل بآلية أخرى
+
+423
+00:34:32,710 --> 00:34:36,290
+كلهم بيقتلوا الأجسام الغريبة و الكائنات الغريبة
+
+424
+00:34:36,290 --> 00:34:41,180
+اللي بتدخل لجسمنا ولكن الآلية مختلفةطبعا؟ إذا إحنا
+
+425
+00:34:41,180 --> 00:34:48,360
+عندنا جيش مدرب للتعرف على الكائنات الغريبة و هذا
+
+426
+00:34:48,360 --> 00:34:53,860
+الجيش .. الجنود تبعته مسلحة بأسلحة مختلفة .. طبعا؟
+
+427
+00:34:53,860 --> 00:34:58,340
+لنيوتروفيل، لإيزينوفيل، لبيزوفيل، لمونوسايتس و
+
+428
+00:34:58,340 --> 00:35:02,580
+للمفوسايتس كل واحدة منهم عندها سلاح مختلف ..
+
+429
+00:35:02,580 --> 00:35:06,760
+مختلفة عن الأخرى في .. لحتى تقتل الكائنات الغريبة
+
+430
+00:35:06,760 --> 00:35:12,290
+فيهطيب مكوّن آخر من مكوّن ال buffy coat احنا حكينا
+
+431
+00:35:12,290 --> 00:35:15,530
+ال buffy coat فيه white pieces وفيه blatelet
+
+432
+00:35:15,530 --> 00:35:20,970
+البلاتلت هي عبارة عن الصفائح الدموية طبعا هي عبارة
+
+433
+00:35:20,970 --> 00:35:27,190
+عن not true cell مش خلايا حقيقية ولكن هي عبارة عن
+
+434
+00:35:27,190 --> 00:35:32,530
+fragment of cytoplasm from certain bone marrow
+
+435
+00:35:32,530 --> 00:35:39,600
+cells اش يعني؟ خلينا نرجع هنا للصورةنشوف هنا في
+
+436
+00:35:39,600 --> 00:35:46,120
+خلايا موجودة داخل البون مارو اسمها Mega Cario
+
+437
+00:35:46,120 --> 00:35:49,860
+Sites تمام؟ خليني أورجيكم إياها هنا لو أنا بدي
+
+438
+00:35:49,860 --> 00:35:58,720
+أشوف أفتح Mega Cario Sites
+
+439
+00:35:58,720 --> 00:36:04,560
+تمام؟ Mega Cario Sites نعمل
+
+440
+00:36:04,560 --> 00:36:09,260
+searchتمام هى ال .. ال .. ال mega-karyocytes خلينى
+
+441
+00:36:09,260 --> 00:36:14,080
+أشوف ال .. ال .. نتيجة الصور هى خلايا ال mega
+
+442
+00:36:14,080 --> 00:36:17,140
+-karyocytes هى خلايا كبيرة طبعا هى أكبر خلية فى
+
+443
+00:36:17,140 --> 00:36:20,860
+البولمارو شايفينها طبعا mega-karyocytes هى mega
+
+444
+00:36:20,860 --> 00:36:24,860
+-karyocytes طبعا هاد ال mega-karyocytes يا بنات هى
+
+445
+00:36:24,860 --> 00:36:28,860
+هى هنا طبعا موجود هاد ال mega-karyocytesبتروح ..
+
+446
+00:36:28,860 --> 00:36:32,160
+موجودة داخل ال bone marrow بتروح عن طرفي ال bone
+
+447
+00:36:32,160 --> 00:36:35,900
+marrow و بيصير ال cytoplasm تبعها يتفتفت و يعطيني
+
+448
+00:36:35,900 --> 00:36:38,880
+جثيمات صغيرة، هذه الجثيمات الصغيرة بتطلع على الدم
+
+449
+00:36:38,880 --> 00:36:43,220
+من برا و بسميها قبلاتلة، صفائح دموية، طبعا هذا يا
+
+450
+00:36:43,220 --> 00:36:46,950
+بنات أنا ما .. مش ..مش طلباكم فيه يعني بس انا عشان
+
+451
+00:36:46,950 --> 00:36:49,990
+اوضحلكم الفكرة هذا ان شاء الله بتدرسوه في مساقات
+
+452
+00:36:49,990 --> 00:36:54,510
+متقدمة لكن انا زي ما بحكيلكم هذه الخلايا ال mega
+
+453
+00:36:54,510 --> 00:36:58,950
+-karyocyte تمام؟ ال mega-karyocyte هاي موجودة داخل
+
+454
+00:36:58,950 --> 00:37:04,110
+البون مارو تمام؟ و أحد مكونات خلايا البون ماروو
+
+455
+00:37:04,110 --> 00:37:09,510
+هذا ال .. ال mega-karyocyte بتروح على طرف البون و
+
+456
+00:37:09,510 --> 00:37:12,790
+لأول ما تقترب من طرف البون بيصير ال cytoplasm
+
+457
+00:37:12,790 --> 00:37:18,110
+تبعها يتفتفت يعطيني جثيمات segments قطع هذه القطع
+
+458
+00:37:18,110 --> 00:37:21,690
+بسميها بلاتلت الصفائح الدموية هذه القطع بسميها إيش
+
+459
+00:37:21,690 --> 00:37:26,550
+الصفائح الصفائح الدموية إذا الخلية إيش اسمها؟
+
+460
+00:37:26,550 --> 00:37:31,450
+الخلية الأم للصفائح الدموية اسمها mega-karyocyte
+
+461
+00:37:31,710 --> 00:37:36,710
+موجودة وين؟ داخل البونمارو، مش موجودة في الدم، لكن
+
+462
+00:37:36,710 --> 00:37:42,050
+هي الcytoplasm تبعها بيتفتفت داخل البونمارو وبيطلع
+
+463
+00:37:42,050 --> 00:37:46,910
+الفتاة .. الفتاة هادا أو الجثيمات هادى بيطلع لأ
+
+464
+00:37:46,910 --> 00:37:52,490
+الدم وعبارة عن إيش؟ بليتلت صفائح دموية طبعا
+
+465
+00:37:52,490 --> 00:37:55,950
+الصفائح دموية كلنا بنعرف إنها إلها دور وين في
+
+466
+00:37:55,950 --> 00:38:01,820
+التخثر بليapart in the clotting of blood إلها دور
+
+467
+00:38:01,820 --> 00:38:05,820
+في ال clotting في التخثر للدم طب خلينا نشوف إيش
+
+468
+00:38:05,820 --> 00:38:10,920
+دورها في التخثر؟ هلأ هاي القصة هادي story تمام عن
+
+469
+00:38:10,920 --> 00:38:14,840
+ال blood clotting و دور البلازمة و دور البلاتلت في
+
+470
+00:38:14,840 --> 00:38:20,600
+ال clotting تمام؟هلأ لنفرض if a damage occur to a
+
+471
+00:38:20,600 --> 00:38:26,120
+blood vessel او circuit .. فإذا صار في عندى خدش أو
+
+472
+00:38:26,120 --> 00:38:31,140
+damage أو جرح أو cut أو injury لل blood vessel
+
+473
+00:38:31,140 --> 00:38:34,440
+تمام؟ يعني مثلا لو انت جرحت أصبعك ما هذا صار فيه
+
+474
+00:38:34,440 --> 00:38:40,580
+تهتك للأوعيات دموية صح؟ عشان هيك بينزل bloodتمام؟
+
+475
+00:38:40,580 --> 00:38:43,460
+فبالتالي إذا صار في عندي damage لهذا ال blood
+
+476
+00:38:43,460 --> 00:38:49,220
+vessel فبقناها ال circulating platelet الصفائح
+
+477
+00:38:49,220 --> 00:38:53,680
+الدموية اللي بتمشي في ال circulation في الدورة
+
+478
+00:38:53,680 --> 00:38:58,400
+الدموية تمام؟ بتروح على طول على منطقة الجرح
+
+479
+00:38:58,400 --> 00:39:03,780
+بيصيرلها trapped at the injury site بتعلق و بتكون
+
+480
+00:39:03,780 --> 00:39:09,450
+موجودة بتلتصق بال injury site في مكان الجرحطبعا
+
+481
+00:39:09,450 --> 00:39:14,050
+هذه ال blatlet بتتراكم و بتعملي حاجة اسمها blatlet
+
+482
+00:39:14,050 --> 00:39:19,830
+blog طبعا بترتبط مع بعض ارتباط وثيق و بتعطيني جلطة
+
+483
+00:39:19,830 --> 00:39:27,040
+او يعني plot ابتدائية اسمها blatlet blogطبعا طيب
+
+484
+00:39:27,040 --> 00:39:31,380
+هذه الخلائق الصفائح الدموية اللي تراكمت وارتبطت مع
+
+485
+00:39:31,380 --> 00:39:35,360
+بعضها البعض و سكرت مكان الجروح بسميها platelet
+
+486
+00:39:35,360 --> 00:39:40,060
+plug طبعا بتغير شكل ال platelet لما بترتبط على
+
+487
+00:39:40,060 --> 00:39:46,040
+مكان الجروح وبيتغير السطح تبعها فبيصير هذا السطح
+
+488
+00:39:46,040 --> 00:39:52,670
+مناسب لحتىإنه بروتينات التخثر اللي موجودة في ال
+
+489
+00:39:52,670 --> 00:39:57,930
+plasma، في الدم، تمام؟ إنها .. إذا لمست سطح
+
+490
+00:39:57,930 --> 00:40:01,930
+البلاتلت تتنشط، هى بتكون موجودة طبعا بروتينات
+
+491
+00:40:01,930 --> 00:40:07,950
+التخثر بتكون موجودة تسير في الدم خاملة، غير ناشطة،
+
+492
+00:40:07,950 --> 00:40:13,560
+لكنإذا لامست سطح البلاتلت ال plug سطح البلاتلت
+
+493
+00:40:13,560 --> 00:40:19,640
+اللى تراكمت على ال injury site تمام و اتغير شكلها
+
+494
+00:40:19,640 --> 00:40:24,820
+و اتغير مكونات سطحها فهذه البروتينات إذا لامست هذا
+
+495
+00:40:24,820 --> 00:40:30,460
+السطح فبتتحول من خاملة إلى ناشطةطبعا يا بنات أنا
+
+496
+00:40:30,460 --> 00:40:34,180
+كام protein عندي؟ طبعا هي سلسلة من البروتينات مش
+
+497
+00:40:34,180 --> 00:40:37,720
+البروتين ولا اتنين ولا تلاتة مش عامل ولا عاملين
+
+498
+00:40:37,720 --> 00:40:43,680
+ولا تلاتة للتخثر ان عندي عوامل للتخثر ما يقارب من
+
+499
+00:40:43,680 --> 00:40:46,660
+اتناش لاربعتاش هتاخدوهم بالتفاصيل يعني انا مابدي
+
+500
+00:40:46,660 --> 00:40:51,260
+افوتلكم بتفاصيل هتاخدوهم في مساقات لاحقة لكن هي
+
+501
+00:40:51,260 --> 00:40:56,380
+عبارة عن سلسلة من البروتينات تمام؟ وهي البروتينات
+
+502
+00:40:58,030 --> 00:41:03,070
+بتتنشط كسلسلة، يعني مثلا أول حاجة العامل رقم 12
+
+503
+00:41:03,070 --> 00:41:11,710
+بنشط 11، 11 طبعا بنشط 9، 9 بنشط 8، 8 بنشط 7 و
+
+504
+00:41:11,710 --> 00:41:18,290
+هكذا، فإذا بيصير سلسل، فالمحصلة إنه بنصل لعامل رقم
+
+505
+00:41:18,290 --> 00:41:22,050
+2 اللي هو ال thrombinتمام؟ بيكون هو عبارة عن
+
+506
+00:41:22,050 --> 00:41:25,950
+ثرومبين .. ثرومبينوجين بيتنشط و بيتكوّن لثرومبين
+
+507
+00:41:25,950 --> 00:41:29,970
+ال ثرومبين هو النوع النشط منه تمام؟ من العامل رقم
+
+508
+00:41:29,970 --> 00:41:34,590
+اتنين هذا العامل رقم اتنين اللي اسمه ثرومبين بيروح
+
+509
+00:41:34,590 --> 00:41:39,730
+بنشط للعامل رقم واحد اللي هو اسمه فيبرينوجين تمام؟
+
+510
+00:41:39,730 --> 00:41:45,000
+ال فيبرينوجين هذا اشماله عبارة عنRod shape
+
+511
+00:41:45,000 --> 00:41:50,120
+syllable protein هو protein ذائب وشكله شكل الرد
+
+512
+00:41:50,120 --> 00:41:57,020
+شكله شكل العصايا يعني شكل رد تمام وموجود في الدم
+
+513
+00:41:57,020 --> 00:42:03,180
+بشكل syllable ذائب تمام إذا تنشط هذا ال fibrinogen
+
+514
+00:42:03,840 --> 00:42:09,800
+عبر ال .. ال thrombin و اتحول ل fibrin الشكل النشط
+
+515
+00:42:09,800 --> 00:42:16,200
+منه فهذا ال fibrin بيكون insolable تمام؟ و هذا ال
+
+516
+00:42:16,200 --> 00:42:20,270
+.. ال protein بيترسب و بيعمللي شبكةبتمسك
+
+517
+00:42:20,270 --> 00:42:24,090
+البروتينات و بتمسك الخلايا ال RBCs بيصيرلها
+
+518
+00:42:24,090 --> 00:42:28,070
+trapped بتتعلق داخل هذه الشبكة و بيكون ليه؟ اللي
+
+519
+00:42:28,070 --> 00:42:32,070
+هي شكل الجلطة الدموية اللي احنا بنشوفها بالشكل ال
+
+520
+00:42:32,070 --> 00:42:37,750
+solid بالشكل الصلب أصبح تم استهلاك ال fibrinogen
+
+521
+00:42:37,750 --> 00:42:42,910
+فالدم اذا اتجلط تمام في الجلطة طبعاتم استهلاك
+
+522
+00:42:42,910 --> 00:42:47,250
+واتحول ل-fibrin وارتبط مع .. مع البلاك اللي تبلغ و
+
+523
+00:42:47,250 --> 00:42:52,690
+.. فعملي جلطة، تمام؟ فأصبح السائل اللي .. اللي
+
+524
+00:42:52,690 --> 00:42:57,070
+موجود في أصل .. ف .. فوق الدم المتجلط معناه إيش
+
+525
+00:42:57,070 --> 00:43:02,130
+اسمه serum ولا يحتوي على fibrinogen ولا يحتوي على
+
+526
+00:43:02,130 --> 00:43:08,600
+عوامل التخثر الأخرى، تمام؟ هذا هو الفرقطيب فإذا ال
+
+527
+00:43:08,600 --> 00:43:12,940
+combined action of a platelet and fibrin web اللي
+
+528
+00:43:12,940 --> 00:43:17,360
+.. اللي .. الش .. اللي .. خليني أرجع لل slide
+
+529
+00:43:17,360 --> 00:43:23,500
+sorryالعمل أو ال .. ال .. ال action، الأثر تبع ال
+
+530
+00:43:23,500 --> 00:43:28,560
+blatlet، الصفائح الدموية و شبكة ال fibrin، تمام؟
+
+531
+00:43:28,560 --> 00:43:34,120
+هذا معناها أنه بيكونلي clot، هذه clot كفيلة بإنها
+
+532
+00:43:34,120 --> 00:43:38,500
+تمنعلي أنه يصير في عندي blood loss في جسم الإنسان
+
+533
+00:43:38,760 --> 00:43:43,760
+لإنها بت .. بتثبت الجلطة و بتسكر مكان الجروح و
+
+534
+00:43:43,760 --> 00:43:49,680
+بتظل موجودة لحد الالتئام لما بيصير الالتئام للجروح
+
+535
+00:43:49,680 --> 00:43:54,960
+و يصير فيه repair طبعا ل .. ال tissue ل ال .. ال
+
+536
+00:43:54,960 --> 00:43:59,800
+blood vessel طبعا يصير له repair يرجع يعني يتعمر و
+
+537
+00:43:59,800 --> 00:44:06,040
+يشتغل و يصير intact و يرجع سليم طبعا فهذه لحد ما
+
+538
+00:44:06,040 --> 00:44:11,250
+يصير فيه repair and healingوشفاء معناها ال .. ال
+
+539
+00:44:11,250 --> 00:44:14,810
+.. ال clot هي راح تظل موجودة و راح تظل تمنعلي ال
+
+540
+00:44:14,810 --> 00:44:18,550
+loss of blood اذا هذه ال clot ايش معناها؟ ممكن
+
+541
+00:44:18,550 --> 00:44:23,850
+تكون life saving يعني بتحمي الحياة احنا في ناس
+
+542
+00:44:23,850 --> 00:44:27,350
+كتير ممكن انهم يموتوا بسبب النزيف بسبب انهم هم
+
+543
+00:44:27,350 --> 00:44:32,110
+ماعندهم مش امكانية او الجسم هم مش قادر يوجف النزيف
+
+544
+00:44:32,110 --> 00:44:38,480
+هذا فالناس اللي عندهم impairedeclote formation
+
+545
+00:44:38,480 --> 00:44:41,380
+طبعا the formation of a clot is impaired او
+
+546
+00:44:41,380 --> 00:44:45,420
+impaired clot formation نتيجة انه مشكلة اما في
+
+547
+00:44:45,420 --> 00:44:50,920
+عوامل التخثر او مشكلة في البلاتلات هدول طبعا at
+
+548
+00:44:50,920 --> 00:44:53,980
+great risk of bleeding to death يعني ممكن يكون
+
+549
+00:44:53,980 --> 00:44:58,040
+عندهم خطر نظيف حتى الموت زي الناس اللي بتعاني من
+
+550
+00:44:58,040 --> 00:45:02,720
+المرض الوراثي ال hemophilia اللي بيكون فيه نقص في
+
+551
+00:45:02,720 --> 00:45:07,880
+عامل التخثر رقم تمانيةتمام؟ فرقم تمانية لما بي ..
+
+552
+00:45:07,880 --> 00:45:13,100
+مهم جدا العامل هذا في .. في السلسلة التخثر لحد ما
+
+553
+00:45:13,100 --> 00:45:17,260
+يتكون عندى ال fibrin إذا هذا العامل مش موجود معناه
+
+554
+00:45:17,260 --> 00:45:21,560
+أنا مش هحصل على fibrin فبالتالي ان ال .. اشخاص ..
+
+555
+00:45:21,560 --> 00:45:26,050
+فبالتالي ال .. ال ..ال .. ال platelet plug لحالها
+
+556
+00:45:26,050 --> 00:45:30,750
+اللي اتكونت مابتقدر انها تسد ال .. ال injury
+
+557
+00:45:30,750 --> 00:45:35,070
+فبالتالي مابتقدر توقف النزيف فبالتالي انه ممكن
+
+558
+00:45:35,070 --> 00:45:37,710
+يصير في عندهم نزيف حتى ال mouth احيانا في بعض
+
+559
+00:45:37,710 --> 00:45:42,350
+الجروح لازمإنه يعني يكون فيه .. طبعا ال blatelet
+
+560
+00:45:42,350 --> 00:45:48,210
+plug بتيجي موجودة بتسد المكان الجرح ولكن ال fibrin
+
+561
+00:45:48,210 --> 00:45:52,150
+clot أو ال fibrin web اللي هي شركة .. شبكة ال
+
+562
+00:45:52,150 --> 00:45:56,450
+fibrin بتيجي و بتتراكم على سطح ال blatelet أصلا هي
+
+563
+00:45:56,450 --> 00:45:59,430
+العوامل اللي بتتنشط على سطح ال blatelet فبتيجي
+
+564
+00:45:59,430 --> 00:46:05,480
+بتتراكم على سطح ال blatelet فبالتاليبت .. بتكونلي
+
+565
+00:46:05,480 --> 00:46:12,280
+شبكة و الشبكة هذه غير ذائبة و قوية فبتدعم و بتقوي
+
+566
+00:46:12,280 --> 00:46:16,480
+الجلطة لحد ما يصير في عندي repair and healing مثل
+
+567
+00:46:16,480 --> 00:46:22,320
+ما حكيناطيب إذا أنواع خلايا الدم erythrocyte خلايا
+
+568
+00:46:22,320 --> 00:46:27,100
+الدم الحمراء dyscotic at the top side وbiconcave
+
+569
+00:46:27,100 --> 00:46:35,520
+at the side view تمام وفي عندي خلايا دم حمراء إذا
+
+570
+00:46:35,520 --> 00:46:39,740
+المسئولة عن ال oxygen delivery في عندي خلايا الدم
+
+571
+00:46:39,740 --> 00:46:43,540
+البيضاء اللي هي عبارة عن lymphocytes خلايا ال
+
+572
+00:46:43,540 --> 00:46:47,150
+lymphocytes بأحجامهاخلايا ال monocytes اللي هي
+
+573
+00:46:47,150 --> 00:46:49,770
+عبارة عن إيش حكينا عن ال lymphocyte و ال monocyte
+
+574
+00:46:49,770 --> 00:46:55,050
+حكينا إنهم هم a granulocyte خلايا ال bezophil و ال
+
+575
+00:46:55,050 --> 00:46:57,930
+ezinophil و ال neutrophil هاي شايفين أنتوا
+
+576
+00:46:57,930 --> 00:47:01,270
+الحبيضات واضحة في ال cytoplasm تبعها فإذن هي خلايا
+
+577
+00:47:01,270 --> 00:47:08,410
+granulocyte بسميها طيب نيجي لإشي مهم اللي هو عبارة
+
+578
+00:47:08,410 --> 00:47:13,890
+عن ال anticoagulantكلمة coagulation لحالها هذه
+
+579
+00:47:13,890 --> 00:47:20,150
+coagulants يعني تجلط، تمام؟ anticoagulants يعني
+
+580
+00:47:20,150 --> 00:47:26,650
+عوامل ماني على التجلطهلأ إحنا في حصات اللي .. اللي
+
+581
+00:47:26,650 --> 00:47:31,830
+تجرى لتقييم محتويات الدم تمام؟ اللي بسميها ال
+
+582
+00:47:31,830 --> 00:47:35,530
+hematology and coagulation test أو hematology and
+
+583
+00:47:35,530 --> 00:47:38,670
+coagulation procedures الحصات اللي بتجرى عليها
+
+584
+00:47:38,670 --> 00:47:42,250
+عينة الدم لحتى أنا أقيم فيها مكونات الدم إما بدي
+
+585
+00:47:42,250 --> 00:47:45,930
+أعد خلايا الدم أو أشوف نسبة ال homoglobin في الدم
+
+586
+00:47:45,930 --> 00:47:49,670
+أو بدي أشوف نسبة عوامل التخثر في الدم أو نشاطها أو
+
+587
+00:47:49,670 --> 00:47:53,710
+تركيزها فهذه كلها اللي بتقيم لي وظائف الدم تمام؟
+
+588
+00:47:53,880 --> 00:47:58,340
+فبالتالي كلها تجرى على إيش؟ تجرى على عينة إما
+
+589
+00:47:58,340 --> 00:48:04,880
+اسمها whole blood يعني دم كامل تمام أو plasma أصبح
+
+590
+00:48:04,880 --> 00:48:07,980
+طالما .. طالما أنه أنا بدي أحصل على plasma إذا
+
+591
+00:48:07,980 --> 00:48:12,700
+لازم الدم اللي أنا بدي أسحبه من المريض إشماله
+
+592
+00:48:12,700 --> 00:48:18,320
+مايتجلطعشان أحصل على plasma طبعا أن الدم .. طبعا
+
+593
+00:48:18,320 --> 00:48:21,720
+الدم إحنا لو سحبناه و تركناه في انبوبة على طول
+
+594
+00:48:21,720 --> 00:48:25,620
+بيتجلط لحاله بتنشط .. تتنشط عوامل التخثر و بتجلط
+
+595
+00:48:25,620 --> 00:48:30,400
+طبعا؟ طيب ف .. لكن أنا بدي أسحبه و بدي أحصل على
+
+596
+00:48:30,400 --> 00:48:35,460
+plasma إذا لازم أضيفله مادة تمنع تجلطه علشان أنا
+
+597
+00:48:35,460 --> 00:48:41,360
+.. يعني ال .. أظلم حافظة على قوامه السائل و لما
+
+598
+00:48:41,360 --> 00:48:46,920
+أحصله أحصل عليه plasmaتمام؟ طيب إذا عشان ال ..
+
+599
+00:48:46,920 --> 00:48:50,600
+الفحوصات إما بتجري على whole blood أو ع plasma
+
+600
+00:48:50,600 --> 00:48:54,440
+يعني أنا بدي الدم يضل سائل عشان .. وضل كامل مخلوط
+
+601
+00:48:54,440 --> 00:48:58,020
+و كامل أو plasma يعني يضل سائل و أنا لما أفصل و
+
+602
+00:48:58,020 --> 00:49:02,580
+أحصل على السائل القشي اللي موجود at the top تمام؟
+
+603
+00:49:02,880 --> 00:49:06,700
+فبالتالي ال blood is withdrawn from the patient،
+
+604
+00:49:06,700 --> 00:49:11,960
+الدم اللي بيسحب من المريض لازم يخلط، يصيرله mixed
+
+605
+00:49:11,960 --> 00:49:15,220
+مع إيش؟ مع anticoagulant، أنا بدي أضيفله
+
+606
+00:49:15,220 --> 00:49:20,800
+anticoagulant، مادة أو عامل مانع للتخثر من كلمة ..
+
+607
+00:49:20,800 --> 00:49:25,200
+من كلمة anti يعني مضاد، تمام؟ coagulants يعني
+
+608
+00:49:25,200 --> 00:49:29,460
+تخثر، مضاد للتخثرعشان إيش أعمل؟ عشان prevent
+
+609
+00:49:29,460 --> 00:49:34,220
+coagulation أمنع تخثر الدم فبالتالي أظل محافظة على
+
+610
+00:49:34,220 --> 00:49:38,720
+الدم سائل فبالتالي أقدر أحصل على whole blood تمام
+
+611
+00:49:38,720 --> 00:49:42,540
+و أحصل على plasma تفيدني في الفحوصات اللي بدي
+
+612
+00:49:42,540 --> 00:49:47,980
+أعملهاتمام؟ أكثر تلت أنواع من ال anticoagulant
+
+613
+00:49:47,980 --> 00:49:52,120
+المستخدمة في مختبرات الهيماتولوجي في مختبرات الدم
+
+614
+00:49:52,120 --> 00:49:57,500
+هما اللي هما ال .. ال .. ال .. ال edita تمام؟ و ال
+
+615
+00:49:57,500 --> 00:50:02,520
+citrate sodium citrate و ال heparin خلينا نشوف إيش
+
+616
+00:50:02,520 --> 00:50:07,120
+مكونات أو إيش خصائص كل واحدة من هذه ال
+
+617
+00:50:07,120 --> 00:50:10,600
+anticoagulant أو المضادات التخطر أو موانع التخطر
+
+618
+00:50:11,140 --> 00:50:15,000
+طيب ال Edita هي عبارة طبعا بتكون احنا .. انا
+
+619
+00:50:15,000 --> 00:50:18,540
+بستخدم أملاح ال Edita طب ال Edita هي عبارة عن
+
+620
+00:50:18,540 --> 00:50:23,240
+اختصار ل إيش؟ اختصار ل ethylene E, ethylene,
+
+621
+00:50:23,680 --> 00:50:29,020
+diamine, tetra, acetic acidهي معنى Edita هيها
+
+622
+00:50:29,020 --> 00:50:33,640
+إثيليين ديامين تترا أسيتك أسد طبعا أنا حكيتلكم
+
+623
+00:50:33,640 --> 00:50:37,480
+باخد أملاح ال Edita يعني هذه ال .. ال إثيليين
+
+624
+00:50:37,480 --> 00:50:41,700
+ديامين تترا أسيتك أسد ال أسيتك أسد هذا بيكون مرتبط
+
+625
+00:50:41,700 --> 00:50:45,940
+بالآخر مع إيش إما مع ال Sodium أو Dipotassium أو
+
+626
+00:50:45,940 --> 00:50:51,290
+Tripotassium تمام؟إذا ال Edeta أنا باخد أملاحها
+
+627
+00:50:51,290 --> 00:50:54,390
+بستخدم الأملاح ال Edeta يعني يا إما ال Edeta دي
+
+628
+00:50:54,390 --> 00:50:57,570
+بتكون مرتبطة مع Sodium أو Di-Botassium أو Tri
+
+629
+00:50:57,570 --> 00:51:00,990
+-Botassium طب إيش هي ال Edeta؟ Ethylene, Diamine,
+
+630
+00:51:01,130 --> 00:51:05,230
+Tetraacetic acid بيكون غالبا تركيزها واحد و نص
+
+631
+00:51:05,230 --> 00:51:08,830
+plus minus ربع يعني من واحد و ربع لواحد point سبعة
+
+632
+00:51:08,830 --> 00:51:13,560
+خمسةتمام؟ هذا التركيز اللي .. اللي .. اللي بيستخدم
+
+633
+00:51:13,560 --> 00:51:19,500
+في المختبرات طبعا ال EDTA بتمنع التخثر من خلال ash
+
+634
+00:51:19,500 --> 00:51:22,380
+طبعا يا بنات احنا .. انا حكيتلكم في .. في عملية
+
+635
+00:51:22,380 --> 00:51:25,940
+التخثر الدم عندي سلسلة من البروتينات اللي بسميها
+
+636
+00:51:25,940 --> 00:51:30,460
+عوامل تخثر أو عوامل تجلط أو بروتينات تجلط بتتنشط
+
+637
+00:51:30,460 --> 00:51:35,140
+كسلسلة وفي المحصلة في النهاية اخر step فيها انه ال
+
+638
+00:51:35,140 --> 00:51:38,240
+fibrinogen بتتحول لfibrin عن طريق ال atherombin
+
+639
+00:51:39,110 --> 00:51:42,470
+تمام؟ هلأ التفاصيل هذه العملية كلها هتاخدوها في
+
+640
+00:51:42,470 --> 00:51:48,070
+مساق اسمه مساق علم النظف والتجلط لكن ..بس انتوا
+
+641
+00:51:48,070 --> 00:51:51,110
+معنيين انكوا تفهموا ان في بروتينات اسمها بروتينات
+
+642
+00:51:51,110 --> 00:51:54,710
+تخثر و بتتنشط كسلسلة يعني واحد بنشط التاني التاني
+
+643
+00:51:54,710 --> 00:51:58,270
+بنشط التالت التالت بنشط هكذا في المحصلة انا بحصل
+
+644
+00:51:58,270 --> 00:52:02,190
+على انه في النهاية عامل التجلط رقم اتنين اللي هو
+
+645
+00:52:02,190 --> 00:52:05,470
+thrombin بنشط عامل التجلط رقم واحد اللي هو ال
+
+646
+00:52:05,470 --> 00:52:08,350
+fibrinogen و بيحولوا ل fibrin ال fibrinogen
+
+647
+00:52:08,350 --> 00:52:14,050
+soluble لكن ال fibrin insoluble تمام؟ طيبهلأ هذه
+
+648
+00:52:14,050 --> 00:52:18,530
+البروتينات وهي عامالها بتنشط بعض بتكون محتاجة ل ..
+
+649
+00:52:18,530 --> 00:52:23,550
+ايونات الكالسيوم محتاجة لإيش؟ للكالسيوم ايون اللي
+
+650
+00:52:23,550 --> 00:52:29,690
+موجودة في الدم طيب لو انا حطي .. اضيفت للدم مادة
+
+651
+00:52:29,690 --> 00:52:35,430
+زي الاديتا تعتبر كمصيادة لالكالسيوم يعني بتاخدكل
+
+652
+00:52:35,430 --> 00:52:39,050
+.. تسحب كل ال calcium اللي موجود في العينة .. في
+
+653
+00:52:39,050 --> 00:52:43,770
+عينة الدم أصبح معناها عوامل التخثر مش راح تلاقي ال
+
+654
+00:52:43,770 --> 00:52:48,130
+.. ال .. ال .. ال calcium كعامة .. ك co-factor
+
+655
+00:52:48,130 --> 00:52:53,650
+لإلها فبالتالي مش هتتنشط تمام؟ فمش هتنشط بعضها
+
+656
+00:52:53,650 --> 00:52:57,370
+فبالتالي مش ه .. في المحصلة مش هيتكون ال fibrin
+
+657
+00:52:57,370 --> 00:53:01,750
+فبالتالي مش راح يتخسر الدم تمام؟ إذا كيف ال Edita
+
+658
+00:53:01,750 --> 00:53:08,650
+بتمنعإنها .. إن الدم يتخثر عن طريق إنها ترتبط مع
+
+659
+00:53:08,650 --> 00:53:11,910
+ال calcium اللي موجود في الدم ال calcium ضروري
+
+660
+00:53:11,910 --> 00:53:16,510
+required for blood coagulation ضروري بيلزم عوامل
+
+661
+00:53:16,510 --> 00:53:20,820
+التخثر و هي عمالها بتنشف بعضهامافيش calcium عوامل
+
+662
+00:53:20,820 --> 00:53:24,220
+التخفر مش راح تتنشط ولا تتنشط بعضها فبالتالي مش
+
+663
+00:53:24,220 --> 00:53:27,060
+راح يصير فيه coagulation في الدم فبالتالي راح يظل
+
+664
+00:53:27,060 --> 00:53:31,420
+الدم سائل فبالتالي طالما انه ظل الدم حافظ على
+
+665
+00:53:31,420 --> 00:53:35,960
+قوامه سائل معناه انا بقدر احصل على whole blood ل
+
+666
+00:53:35,960 --> 00:53:40,300
+الفحوصات و بقدر انا لو حطته ع جهاز ال centrifuge
+
+667
+00:53:40,300 --> 00:53:44,140
+وفصلته السائل القشي اللي موجود at the top طالما
+
+668
+00:53:44,140 --> 00:53:47,320
+الدم مش متجلة اسمه plasma اذا بينفعني في الفحوصات
+
+669
+00:53:47,320 --> 00:53:54,160
+الهيماتولوجيةتمام؟ طيب خلينا نشوف قبل أحكي عن هذه
+
+670
+00:53:54,160 --> 00:53:57,640
+النقطة إيش هي .. كيف شكل الأنابيب اللي أنا بجمع
+
+671
+00:53:57,640 --> 00:54:02,940
+فيها عينة الدم خلينا نشوف هاي هنا ال tube of blood
+
+672
+00:54:02,940 --> 00:54:07,340
+هذه طبعا أنابيب مختلفة فيها anticoagulant مختلف
+
+673
+00:54:07,340 --> 00:54:13,220
+تمام؟ و نجمع فيها عينة الدم كل انبوب بتكون متخصصة
+
+674
+00:54:13,220 --> 00:54:19,120
+لفحص معينمثلا في عندى بيكون سترات في بيكون ال ..
+
+675
+00:54:19,120 --> 00:54:26,720
+heparin هى edita هى طبعا لل ESR سترات و هى هنا فيه
+
+676
+00:54:26,720 --> 00:54:30,400
+plain يعنى مافيش فيها حاجة خالص طبعا فاضية يعنى
+
+677
+00:54:30,400 --> 00:54:33,520
+الدم هيتجلط فيها مدى مافيش فيها anticoagulant الدم
+
+678
+00:54:33,520 --> 00:54:36,680
+هيتجلط فيها و لما أفصلها في ال centrifuge هتعطينى
+
+679
+00:54:36,680 --> 00:54:40,240
+serum مش plasma طبعا اذا هذه هي الأنابيب اللي هى
+
+680
+00:54:40,240 --> 00:54:44,890
+color .. color code يعنى كل لونعبارة بيدللي إن
+
+681
+00:54:44,890 --> 00:54:48,810
+محتوياته مختلفة تمام في ال anticoagulant اللي
+
+682
+00:54:48,810 --> 00:54:52,370
+موجود فيه مختلف إما بيكون فيها anticoagulant مختلف
+
+683
+00:54:52,370 --> 00:54:55,570
+أو بيكونش فيها anticoagulant خالص زي البلان هاي
+
+684
+00:54:55,570 --> 00:55:00,050
+الحمرة تمام فبالتالي إما بتفصللي plasma أو بتفصللي
+
+685
+00:55:00,050 --> 00:55:04,730
+serum لما أحطها على جهاز ال centrifuge فبالتالي
+
+686
+00:55:04,730 --> 00:55:09,510
+حسب إيش مكوناتها أو إيش محتويات ال tubeتمام؟ يا
+
+687
+00:55:09,510 --> 00:55:12,770
+بنات كمية ال anticoagulant اللي موجودة أحيانا
+
+688
+00:55:12,770 --> 00:55:18,700
+بتكون يعني لا تكتت الكارويعني أحيان ما بتشوفيها ..
+
+689
+00:55:18,700 --> 00:55:21,860
+ممكن تشوفيها ك powder أو تشوفيها كقطرات على جدار
+
+690
+00:55:21,860 --> 00:55:25,640
+التيوب و ممكن ما تشوفيها، ما تلاحظيها، لكن هي
+
+691
+00:55:25,640 --> 00:55:29,100
+بيكون موجودة فيها كميات قليلة من ال anticoagulant
+
+692
+00:55:29,100 --> 00:55:33,940
+و بالتالي أيضا مابيكون معلملي أنه أنا أسحب الدم
+
+693
+00:55:33,940 --> 00:55:36,560
+لعند علامة معينة في التيوب هذه، يعني أنا لو بدي
+
+694
+00:55:36,560 --> 00:55:41,760
+أفض التيوب داخل أو أفض blood داخل التيوب، بدي
+
+695
+00:55:41,760 --> 00:55:46,130
+أفضيه عند علامة معينةبحيث أنه يظل نسبة الدم لل
+
+696
+00:55:46,130 --> 00:55:48,890
+anticoagulant ثابتة مائية يعني ال anticoagulant
+
+697
+00:55:48,890 --> 00:55:53,250
+هذا ملح بيأثر أحيانا على شكل الخلايا بيأثر على
+
+698
+00:55:53,250 --> 00:55:56,370
+المكونات
+
+699
+00:55:56,370 --> 00:56:01,370
+الدم فبالتالي لازم أنا أظلم حافظة على النسبة ما
+
+700
+00:56:01,370 --> 00:56:04,830
+بين الدم و عامل ال anticoagulant هذا بحيث أني
+
+701
+00:56:04,830 --> 00:56:08,490
+ماأثر على محتويات الدم ببالتالي ماأحصل على نتائج
+
+702
+00:56:08,490 --> 00:56:12,190
+خاطئة في الفحوصات يعني مثلا هيهانةلـ tube هذه ال
+
+703
+00:56:12,190 --> 00:56:15,890
+tube ال ESR فيها Sodium Citrate تمام ال .. ال
+
+704
+00:56:15,890 --> 00:56:18,310
+anticoagulant اللي موجود اسمه Sodium Citrate
+
+705
+00:56:18,310 --> 00:56:22,010
+هنشتغل فحص ال ESR و هنحكي عن Sodium Citrate
+
+706
+00:56:22,010 --> 00:56:26,410
+Anticoagulant و هيتكرر على ما سامعكم هذا الاسم
+
+707
+00:56:26,410 --> 00:56:30,920
+كتير Sodium Citrate تمام؟طيب شايفين العلامة هذه
+
+708
+00:56:30,920 --> 00:56:36,140
+اللي رونها أسود هذه لحد عندها لازم أنا أعبي blood
+
+709
+00:56:36,140 --> 00:56:40,580
+يعني لحد هنا أعبي blood عشان مايكونش كمية ال ..
+
+710
+00:56:40,580 --> 00:56:44,820
+السترات كمية الملح أكتر في الدم فبالتالي تأثر على
+
+711
+00:56:44,820 --> 00:56:49,680
+شكل الخلايا ومكونات الدماحيانا بيكون إذا ما أعطاني
+
+712
+00:56:49,680 --> 00:56:53,300
+علامة ع الجانب ممكن يكون هذا الخط الملون هو .. هو
+
+713
+00:56:53,300 --> 00:56:57,400
+نفس العلامة تمام؟ هذه شايفين إيش فيها زي الشمع أو
+
+714
+00:56:57,400 --> 00:57:01,820
+زي ال .. ال silicon زي مادة ال silicon جوا؟ هذه
+
+715
+00:57:01,820 --> 00:57:06,080
+بيكون فيها clot activator يعني بتعطيني serum تمام؟
+
+716
+00:57:06,080 --> 00:57:10,220
+بت .. بس إيش .. يعني .. يعني الدم بيتخسر أسرع من
+
+717
+00:57:10,220 --> 00:57:12,640
+هذه هذا الدم .. هذه مافيش فيها حاجة يعني أنا بترك
+
+718
+00:57:12,640 --> 00:57:16,000
+الدم يتخسر على أحتهبعدين لما أفصل في جهاز ال
+
+719
+00:57:16,000 --> 00:57:22,700
+centrifuge بيعطيني ash المكونات انه عبارة عن يعني
+
+720
+00:57:22,700 --> 00:57:27,810
+دم متجلة و أتدى top serumها دي نفس الاشي بيتجلط
+
+721
+00:57:27,810 --> 00:57:32,610
+الدم و لما أفصل بجهاز ال centrifuge بيعطيني ال
+
+722
+00:57:32,610 --> 00:57:36,990
+serum و الدم المتجلط بالأسفل ولكن هنا بيتجلط أثر
+
+723
+00:57:36,990 --> 00:57:41,230
+على إن هذا مادة ال silicon بقرا عن clock activator
+
+724
+00:57:41,230 --> 00:57:47,950
+يعني منشطة لحدوث التخثر تمام؟طيب فإذا بديكم بس
+
+725
+00:57:47,950 --> 00:57:52,790
+تركزوا كتير منيح إنه حجم الدم هنا بيكون طبعا في
+
+726
+00:57:52,790 --> 00:57:57,670
+عندي علامة بتكون موجودة لتحد تتحددلي أنا عبي ال
+
+727
+00:57:57,670 --> 00:58:02,490
+tube هاي ل .. ل .. لامتة أو ل .. لوين في ال blood
+
+728
+00:58:02,490 --> 00:58:06,150
+تمام؟ إما بيكون علامة على الجانب أو الخط الملون
+
+729
+00:58:06,150 --> 00:58:10,990
+هذا نفسه بيكون هو نفس العلامة طيب خلينا نشوف طيب
+
+730
+00:58:10,990 --> 00:58:16,450
+لو أناكان في عندي في ال tube تبعتي excessive
+
+731
+00:58:16,450 --> 00:58:20,450
+concentration of edita اش يعني؟ يعني ال edita
+
+732
+00:58:20,450 --> 00:58:26,190
+زيادة يعني الدم انا سحبته اقل من العلامة طبعا؟
+
+733
+00:58:26,190 --> 00:58:30,690
+مدام في عندي ملح كتير هذا هيأثر على شكل خلايا ال
+
+734
+00:58:30,690 --> 00:58:35,370
+RBCs ويعمللها shrinkage اش يعني shrinkage؟ يعني
+
+735
+00:58:35,370 --> 00:58:42,150
+الخلايا هتتكرمش هيصيرلها كرمشة طبعا؟ و تتقلص
+
+736
+00:58:43,060 --> 00:58:47,200
+فبالتالي هذا هيأثر على الفحوصات الهيماتوكرت هيخليه
+
+737
+00:58:47,200 --> 00:58:54,460
+decreased و هيخلي ال MCHC increased و هيقلل ال ESR
+
+738
+00:58:54,460 --> 00:59:00,120
+تمام؟ كل الفحوصات اللي بتعتمد على حجم الخلايا الدم
+
+739
+00:59:00,120 --> 00:59:03,740
+الحمراء على قياس حجم خلايا الدم الحمراء كلها راح
+
+740
+00:59:03,740 --> 00:59:09,510
+تتأثرdecreased hematocrit و increased MCHC و
+
+741
+00:59:09,510 --> 00:59:13,550
+falsely low E-ASR هدى طبعا اشملها هدى نتائج خاطئة
+
+742
+00:59:13,550 --> 00:59:18,130
+انا حصلت عليها نتيجة ان انا عبت ال blood اقل من
+
+743
+00:59:18,130 --> 00:59:21,890
+العلامة داخل التيوب يعنى انا ماحطت كفاية blood
+
+744
+00:59:21,890 --> 00:59:25,470
+داخل التيوب فبالتالي كمية الادتة كانت زيادة
+
+745
+00:59:25,470 --> 00:59:30,000
+بالنسبة لا ل bloodو أيضا الidity هيالزيادة ممكن
+
+746
+00:59:30,000 --> 00:59:32,780
+تعملي degenerative changes in white cells يعني
+
+747
+00:59:32,780 --> 00:59:37,480
+ممكن تخلي ال white cells انها هي تتحلل ممكن يعملي
+
+748
+00:59:37,480 --> 00:59:43,220
+swelling ل البلاتلت فبالتالي هي ايضا هيخليلي
+
+749
+00:59:43,220 --> 00:59:46,500
+هيعملي falsely و يخليها طبعا لما انها صار لها
+
+750
+00:59:46,500 --> 00:59:51,220
+swelling البلاتلت لما انها انتفخت ممكن تتكسر و لما
+
+751
+00:59:51,220 --> 00:59:58,170
+انا قاعدها على الجهازمفوتات ل blatlet يعدوا الجهاز
+
+752
+00:59:58,170 --> 01:00:02,870
+ك blatlet فبالتالي انه يعملي increase ان blatlet
+
+753
+01:00:02,870 --> 01:00:11,050
+count طبعا؟ طيبSodium citrate النوع التاني اللي هو
+
+754
+01:00:11,050 --> 01:00:15,350
+ال common يعني أكتر شيوعا في المختبرات ال
+
+755
+01:00:15,350 --> 01:00:19,330
+hematology هو عبارة عن Sodium citrate طبعا ملح
+
+756
+01:00:19,330 --> 01:00:25,530
+Sodium citrate بيكون إما موجود ك Sodium citrate
+
+757
+01:00:25,530 --> 01:00:30,930
+dehydrate أو Sodium citrate عادي طبعا طيب فبالتالي
+
+758
+01:00:30,930 --> 01:00:34,810
+أو potassium citrate أحيانافال .. ال sodium
+
+759
+01:00:34,810 --> 01:00:39,150
+citrate هذا دايما انا بحصل .. يعني طبعا لإذا بدي
+
+760
+01:00:39,150 --> 01:00:43,290
+اعمل فحوصات التخثر ال coagulation studies لازم
+
+761
+01:00:43,290 --> 01:00:48,990
+يكون نسبة الدم تسعة أجزاء و ال citrate one يعني
+
+762
+01:00:48,990 --> 01:00:56,120
+نسبة ال anticoagulant لل blood واحد لتسعةتمام؟
+
+763
+01:00:56,120 --> 01:01:00,020
+فإذا مهم جدا حكاية العلامة هذه وكمية الدم اللي أنا
+
+764
+01:01:00,020 --> 01:01:03,700
+بدي أفضيه داخل ال tube بعد ما أسحب العينة Sodium
+
+765
+01:01:03,700 --> 01:01:08,200
+citrate prevent coagulation by binding the calcium
+
+766
+01:01:08,200 --> 01:01:12,020
+to the blood in soluble complex طبعا احنا حكينا
+
+767
+01:01:12,020 --> 01:01:15,660
+انه هذا النوع من ال anticoagulant زي و زي ال Edeta
+
+768
+01:01:15,660 --> 01:01:19,500
+بيمنع التخطر من خلال ارتباطه بال calcium واحنا
+
+769
+01:01:19,500 --> 01:01:24,010
+حكينا انه ال calcium ضروريلتنشيط عوامل التخطر فإذا
+
+770
+01:01:24,010 --> 01:01:27,890
+مافيش calcium معناها عوامل التخطر مش روحت اتنشط
+
+771
+01:01:27,890 --> 01:01:31,750
+فمافيش كلوت فبالتالي الدم رح يظل سائل فإذا عشان
+
+772
+01:01:31,750 --> 01:01:35,950
+هيك بسمي هذه المواد anticoagulant مضادات للتخثر
+
+773
+01:01:35,950 --> 01:01:40,460
+موانع للتخثرتروح ترتبط بالكالسيوم و بتمنع التخثر
+
+774
+01:01:40,460 --> 01:01:46,420
+طيب TND الهيبرين نوع تالت من ال anticoagulant اللي
+
+775
+01:01:46,420 --> 01:01:52,100
+أكتر شيوعا في ال hematology lab ولكن طبعا انا ممكن
+
+776
+01:01:52,100 --> 01:01:58,830
+استخدمها من 15 ل 30 unit per ml تركيزهو طبعا ممكن
+
+777
+01:01:58,830 --> 01:02:01,670
+أحيانا يسببلي clumping ل ال blatelet في ناس عندهم
+
+778
+01:02:01,670 --> 01:02:05,630
+حساسية من ال heparin فبالتالي ممكن أنا إذا ضفتلهم
+
+779
+01:02:05,630 --> 01:02:09,750
+heparin لعينتهم يصير فيه clumping يصير فيه تجمعات
+
+780
+01:02:09,750 --> 01:02:15,650
+ل ال blatelet و ال white pieces فبالتالي هدول
+
+781
+01:02:15,650 --> 01:02:19,970
+الناس أنا إذا لاحظت إنه استخدمت ال anticoagulant
+
+782
+01:02:19,970 --> 01:02:23,630
+هذا ال heparin و روحت أعد خلايا ال blatelet و أعد
+
+783
+01:02:23,630 --> 01:02:26,610
+ال white pieces و لاجتها جليلةبدو يجي في بالي ان
+
+784
+01:02:26,610 --> 01:02:29,230
+هو ممكن يكون هذا المريض بيتحسس من الهيبارين
+
+785
+01:02:29,230 --> 01:02:32,750
+فبالتالي عشان هيك صار في عنده خلايا ال .. ال
+
+786
+01:02:32,750 --> 01:02:35,750
+platelet و ال white بيصير صارلها clumping فبالتالي
+
+787
+01:02:35,750 --> 01:02:39,390
+لازم انا ابدل يعني اصحبله عينة تانية و أحطها في
+
+788
+01:02:39,390 --> 01:02:44,010
+anticoagulant أخر بعدين أعمل count تمام؟ طيب هذه
+
+789
+01:02:44,010 --> 01:02:48,670
+يعني بس يعني من ال limitation او من عيوب الهيبارين
+
+790
+01:02:49,160 --> 01:02:53,540
+طيب ال heparin بيمنع التخثر أيضا، اذا انا ممكن
+
+791
+01:02:53,540 --> 01:02:57,180
+احصل على plasma من عينة الدم اللي مضعفة لهيبارين،
+
+792
+01:02:57,180 --> 01:03:01,860
+ولكن القالية تبعته مختلفة، احنا حاكينا ال Edeta و
+
+793
+01:03:01,860 --> 01:03:06,840
+ال citrate بيرتبطوا بال calcium، طبعا؟ فبيمنع ال
+
+794
+01:03:06,840 --> 01:03:11,340
+.. ال .. ان تنشيط عوامل التخثر، فبالتالي مابيصيش
+
+795
+01:03:11,340 --> 01:03:16,300
+فيه clotطبعا لكن الهيبارين إيش بيروح بعمل بيروح
+
+796
+01:03:16,300 --> 01:03:21,100
+بيشتغل بقالية مختلفة بميكانيزم مختلف الهيبارين
+
+797
+01:03:21,100 --> 01:03:26,340
+بيروح بنشط ال protein اسمه ال antithrombin 3 و هذا
+
+798
+01:03:26,340 --> 01:03:30,420
+ال antithrombin 3 بيروح بيرتبط مع ال thrombin و
+
+799
+01:03:30,420 --> 01:03:34,340
+بعمله inhibition طبعا فطالما ارتبط مع ال thrombin
+
+800
+01:03:34,340 --> 01:03:38,480
+و عملله inhibition مافيش عندي fibrinogen يتحول ل
+
+801
+01:03:38,480 --> 01:03:42,520
+fibrin إذا مافيش عندي clot إذا ال blood هيظل سائل
+
+802
+01:03:42,610 --> 01:03:47,570
+طبعا هذا هو الفارق في القالية ما بين ال .. ال
+
+803
+01:03:47,570 --> 01:03:51,960
+اليتا و الستريات و ما بين الهيبارينطيب ال blood
+
+804
+01:03:51,960 --> 01:03:56,980
+collecting tubes طبعا احنا في عندى عدة انواع من ال
+
+805
+01:03:56,980 --> 01:03:59,780
+tubes اللى هى ال blood collecting tubes هى هنا فيه
+
+806
+01:03:59,780 --> 01:04:02,500
+several types of blood collecting tubes are
+
+807
+01:04:02,500 --> 01:04:08,560
+available و طبعا حكينا انه هى ال .. ال .. ممكن انا
+
+808
+01:04:08,560 --> 01:04:11,760
+اميزها discriminated by their top color حكينا
+
+809
+01:04:11,760 --> 01:04:16,610
+انهيلها color codeكل لون الغطاية أو اللون ال .. ال
+
+810
+01:04:16,610 --> 01:04:20,790
+top تبعها مختلف فبيكون .. كل لون بيعطي .. بي ..
+
+811
+01:04:20,790 --> 01:04:24,630
+بيدللي إن ال .. ال .. ال anticoagulantilli في
+
+812
+01:04:24,630 --> 01:04:30,250
+الداخل مختلفتمام؟ يعني هاي ال .. ال .. ال cube
+
+813
+01:04:30,250 --> 01:04:34,550
+اللي ال top تبعها red طبعاً هذا يا بناتي يعني إشي
+
+814
+01:04:34,550 --> 01:04:38,530
+معمّم، ما .. يعني مابنفعش .. يعني مش مطبق في دولة
+
+815
+01:04:38,530 --> 01:04:43,190
+او دولة لأ، لأ هي كل الأنابيب هيك بتكون، تمام؟
+
+816
+01:04:43,190 --> 01:04:47,330
+فبالتاليالقنابيب اللي ال red .. ال .. ال top تبعها
+
+817
+01:04:47,330 --> 01:04:50,510
+لونه red حكينا إيش بسميها plain tube يعني مافيش
+
+818
+01:04:50,510 --> 01:04:57,410
+فيها أي حاجة فاضية no أو none additive تمام؟ فهذه
+
+819
+01:04:57,410 --> 01:05:01,390
+ال non additive إيش يعني؟ يعني مافيش فيها أي
+
+820
+01:05:01,390 --> 01:05:07,210
+إضافات فالدم هيفصل و هيتجلط و لما أفصل و هيعطيني
+
+821
+01:05:07,210 --> 01:05:11,490
+serum تمام؟ في عندي ال .. ال top اللي لونه orange
+
+822
+01:05:11,490 --> 01:05:18,140
+في .. خلينا نشوف .. نرجع لصورة هنااللي لونه هيك
+
+823
+01:05:18,140 --> 01:05:22,980
+على orange أو على أصفر فيها clot activator تمام؟ و
+
+824
+01:05:22,980 --> 01:05:27,680
+هذا ليه .. ليه clot activator؟ يعني منشط للتخثر
+
+825
+01:05:27,680 --> 01:05:32,660
+فبرضه ال blood هيتجلط فيه و هيعطيني serum و أيضا
+
+826
+01:05:32,660 --> 01:05:36,980
+نفس الاشي اللي .. اللي لونه yellow اللي لونه أصفر
+
+827
+01:05:36,980 --> 01:05:42,300
+صريح برضه كمان فيه clot activator و فيه gelفهدول
+
+828
+01:05:42,300 --> 01:05:47,020
+برضه كمان بينشطوا عملية التجلط ف ال blood هيتجلط و
+
+829
+01:05:47,020 --> 01:05:51,460
+لما أفصله هيعطيني serum تمام؟ ال heparin tube
+
+830
+01:05:51,460 --> 01:05:55,260
+بيكون لونها green في هذا اللون، لون ال mint green
+
+831
+01:05:55,260 --> 01:05:58,960
+اللي هو الأخضر اللي .. اللي ماخد اللون النعناعي
+
+832
+01:05:58,960 --> 01:06:05,100
+تمام؟ heparin tube فهذا ال .. ال tube فيه heparin
+
+833
+01:06:05,100 --> 01:06:08,320
+إذا الدم مش هيتجلط إذا لما أفصله بيعطيني plasma
+
+834
+01:06:08,930 --> 01:06:12,930
+تمام؟ إما heparin sodium أو heparin lithium بيكون
+
+835
+01:06:12,930 --> 01:06:16,970
+فيه .. تمام؟ اللي لونه light green الأخضر الفاتح
+
+836
+01:06:16,970 --> 01:06:21,410
+بيكون موجود فيه gel و heparin lithium تمام؟ و بي
+
+837
+01:06:21,410 --> 01:06:25,590
+.. و .. و بيفصل ال .. طبعا .. طبعا الدم مش هيتخسر
+
+838
+01:06:25,590 --> 01:06:29,030
+مدام فيه heparin فبالتالي لما أفصله بيعطيني plasma
+
+839
+01:06:29,030 --> 01:06:33,490
+إيش يعني plasma؟ يعني أن السائل ما .. ما .. ما تم
+
+840
+01:06:33,490 --> 01:06:37,250
+استهلاكال .. العوامل التخطرى اللى موجودة فى الدم
+
+841
+01:06:37,250 --> 01:06:41,290
+فالسائل القشى اللى موجود فوق ال blood هذا محتوى ..
+
+842
+01:06:41,290 --> 01:06:44,890
+بيحتوي على fibrinogen و لو أنا عملت mix للعينة
+
+843
+01:06:44,890 --> 01:06:49,570
+بترجع دم، بترجع whole blood تمام؟ ال Edita تيوب
+
+844
+01:06:49,570 --> 01:06:55,850
+اللى لونها .. يعني بيكون لونها purple أو ..move او
+
+845
+01:06:55,850 --> 01:06:59,170
+حتى lavender احيانا بيسموها في كتب بتسميها اللون
+
+846
+01:06:59,170 --> 01:07:02,650
+ال lavender فبالتالي هذه اما dibotassium او
+
+847
+01:07:02,650 --> 01:07:07,670
+tributassium او حتى ال sodium edita هذه كلها بتكون
+
+848
+01:07:07,670 --> 01:07:11,310
+بتعطيني اما holy blood او لو فصلتها بتعطيني plasma
+
+849
+01:07:11,310 --> 01:07:15,920
+تمام؟ال blue بيكون فيها sodium citrate بتركيز
+
+850
+01:07:15,920 --> 01:07:20,580
+مختلفة عشان تعطيني ال .. طبعا انا باخد ال plasma
+
+851
+01:07:20,580 --> 01:07:24,660
+تبعتها و بشتغل عليها بحوصات التخثر ال tube اللي
+
+852
+01:07:24,660 --> 01:07:26,820
+بيكون ال tube تبعها black بيكون فيها sodium
+
+853
+01:07:26,820 --> 01:07:31,220
+citrate عشان اشتغل فيها فحص ال ESR و ال glucose
+
+854
+01:07:31,220 --> 01:07:35,040
+tube اللي هي لونها greyبيكون فيها Sodium Fluoride
+
+855
+01:07:35,040 --> 01:07:40,360
+طبعا ال Sodium Fluoride من خصائصه انه هو بيمنع ال
+
+856
+01:07:40,360 --> 01:07:43,200
+glycolysis تبع الخلايا بيمنع ال metabolism تبع
+
+857
+01:07:43,200 --> 01:07:46,720
+الخلايا فما انه بيمنع ال .. ال metabolism تبع ..
+
+858
+01:07:46,720 --> 01:07:50,060
+هو inhibitor للميطابوليزم و بيمنع الميطابوليزم
+
+859
+01:07:50,060 --> 01:07:54,890
+فبالتالي الخلايا مش راح تستهلك glucoseفإذا يفضل
+
+860
+01:07:54,890 --> 01:07:58,630
+إنه أنا لو بدي أشتغل فحص ل glucose أضيء .. أسحب
+
+861
+01:07:58,630 --> 01:08:02,550
+عينة الدم و أضيفها على tube ال top تبعها grey يعني
+
+862
+01:08:02,550 --> 01:08:08,530
+موجود فيها sodium و fluoride زي هذا الشكل هذاتمام؟
+
+863
+01:08:08,530 --> 01:08:11,250
+هي موجودة .. هي مكتوبة هنا glucose يعني فيها
+
+864
+01:08:11,250 --> 01:08:15,750
+Sodium Fluoride تمام؟ هذه هي مكونات .. هذا هو ال
+
+865
+01:08:15,750 --> 01:08:20,010
+color code و هذا المكونات اللي فيها .. الإضافات
+
+866
+01:08:20,010 --> 01:08:23,670
+اللي فيها و هذا نوع العينة اللي بحصل عليه من كل
+
+867
+01:08:23,670 --> 01:08:29,630
+tube الجدول هذا يا بنات مش حفظ رح يكون معاكم طبعا
+
+868
+01:08:29,630 --> 01:08:33,050
+يعني بعض الأشياء حفظ يعني مهم تعرفوا لإن ال planet
+
+869
+01:08:33,050 --> 01:08:35,890
+tube مافيش فيها حاجة و بتعطيني serum يعني الدم
+
+870
+01:08:35,890 --> 01:08:40,780
+بيتخسرال black tube فيها sodium citrate و بتشتغل
+
+871
+01:08:40,780 --> 01:08:44,360
+.. بتنشغل لل ESR ال .. ال .. ال bare ball فيها
+
+872
+01:08:44,360 --> 01:08:48,540
+edita و بتعطيني holy blood و لو فصلتي ال holy
+
+873
+01:08:48,540 --> 01:08:52,940
+blood بحصل على plasma باقي الألوان مع الممارسة و
+
+874
+01:08:52,940 --> 01:08:57,870
+مع التكرار هيمروا معاكم خلال فحوصات ال ..كتيرة في
+
+875
+01:08:57,870 --> 01:09:03,770
+مختبرات التحليل فبالتالي ما بتتخرجوا إلا أنتوا
+
+876
+01:09:03,770 --> 01:09:07,470
+بتعرفوا هاي الأنابيب و بتعرفوا إيش مكوناتها و يمكن
+
+877
+01:09:07,470 --> 01:09:12,470
+حاقظين ألوانها من كتر ما بتستخدموها، تمام؟ فإذا بس
+
+878
+01:09:12,470 --> 01:09:17,950
+اعرفولي حاليا مبدأيا في المعمل تبعنا هذا ال red ال
+
+879
+01:09:17,950 --> 01:09:22,640
+purple ال black تمام؟فهدول بس اللي بتعرفوا .. بدكم
+
+880
+01:09:22,640 --> 01:09:26,280
+تعرفوا ليهم ال verbal ممكن تلاقوا في كتب بتعطيني
+
+881
+01:09:26,280 --> 01:09:30,520
+ان هو اسمه lavender تمام؟ هتمروا يعني معانا
+
+882
+01:09:30,520 --> 01:09:33,920
+chapter بيحكي عن ال verbal هذا او ال .. التيوب هاي
+
+883
+01:09:33,920 --> 01:09:39,520
+lavender فهي نفس المعنى تمام؟ فهدول اللي ..
+
+884
+01:09:42,220 --> 01:09:45,880
+المطالبين أنتوا تحفظوهم طبعا كل cube يا بنات في
+
+885
+01:09:45,880 --> 01:09:49,700
+منها أحجام في بتسحب ..يعني بتكفي لحد اتنين أمقال
+
+886
+01:09:49,700 --> 01:09:53,080
+لتلاتة لاربع لخمسة أمقال طبعا اللي بيحددلي ال
+
+887
+01:09:53,080 --> 01:09:56,300
+volume اللي أنا بدي أعبيه هو العلامة اللي وين
+
+888
+01:09:56,300 --> 01:10:01,220
+معلمة أو اللي وين موجود في علامة داخلال .. ال ..
+
+889
+01:10:01,220 --> 01:10:04,740
+او على .. على جدار ال tube إما على الجانب أو حتى
+
+890
+01:10:04,740 --> 01:10:08,160
+في .. يعني هذا أحيانا بيكون هو نفسه علامة فلوين في
+
+891
+01:10:08,160 --> 01:10:12,780
+علامة عندي، لوين بعبي أنا blood فبالتالي بيحددلي
+
+892
+01:10:12,780 --> 01:10:16,000
+ال volume، طبعا، في tubeات بتكون مصممة ل volume
+
+893
+01:10:16,000 --> 01:10:20,700
+قليل، في tubeات بتكون مصممة ل volume أكترحسب نوع
+
+894
+01:10:20,700 --> 01:10:26,220
+الفحص أو الفحوصات اللي مطلوبة، يعني إذا أنا بدي ..
+
+895
+01:10:26,220 --> 01:10:29,400
+بدي أشتغل من ال YouTube هذي عدة فحوصات، بختار ال
+
+896
+01:10:29,400 --> 01:10:32,740
+YouTube اللي حجم .. بتعطيني حجم أكتر، أما إذا كانت
+
+897
+01:10:32,740 --> 01:10:36,060
+ال .. الفحوصات قليلة فبختار ال YouTubeات اللي بت
+
+898
+01:10:36,060 --> 01:10:40,760
+.. بتعبق أو بيكون فيهاأو بتعطيني حجم أقل هذا
+
+899
+01:10:40,760 --> 01:10:45,660
+بالنسبة لمحاضرتنا لليوم بنكون هيك خلصنا تعرفنا على
+
+900
+01:10:45,660 --> 01:10:50,420
+مكونات الدم و ال anticoagulant و الأنواع الأكتر
+
+901
+01:10:50,420 --> 01:10:55,780
+common و وظائفها تمام؟عندكم أي سؤال، أي استفسار
+
+902
+01:10:55,780 --> 01:11:00,660
+بعتوليا بقى على group ال whatsapp بجاوبكم من خلال
+
+903
+01:11:00,660 --> 01:11:05,700
+تسجيل صوتي أو بنتناقش فيه عبر محاضرة ال zoom اللي
+
+904
+01:11:05,700 --> 01:11:10,440
+موعدها يوم الصبت يعطيكم العافية وموفقين بإذن الله
+
diff --git a/PL9fwy3NUQKwY9jl8xw-bWkVZkWcGGyspd/pYa8qDaMM00.srt b/PL9fwy3NUQKwY9jl8xw-bWkVZkWcGGyspd/pYa8qDaMM00.srt
new file mode 100644
index 0000000000000000000000000000000000000000..4d68d92a828a229e3ffa6712f12410d0421d4013
--- /dev/null
+++ b/PL9fwy3NUQKwY9jl8xw-bWkVZkWcGGyspd/pYa8qDaMM00.srt
@@ -0,0 +1,2657 @@
+
+1
+00:00:03,930 --> 00:00:07,410
+السلام عليكم ورحمة الله وبركاته طالبات العزيزات
+
+2
+00:00:07,410 --> 00:00:10,610
+اليوم إن شاء الله رح نشرح محاضرة بعنوان calorie
+
+3
+00:00:10,610 --> 00:00:15,090
+meter or photometer في البداية ماذا يعني calorie
+
+4
+00:00:15,090 --> 00:00:20,610
+meter؟ color معناها لون، تمام؟ meter قياس، إذا قياس
+
+5
+00:00:20,610 --> 00:00:26,040
+شدة اللون أو photometer، photo معناها ضوء، وmeter
+
+6
+00:00:26,040 --> 00:00:31,540
+قياس، أو قياس شدة الضوء، قياس شدة اللون أو قياس شدة
+
+7
+00:00:31,540 --> 00:00:36,020
+الضوء هي طريقة تستخدم for the concentration،
+
+8
+00:00:36,020 --> 00:00:39,060
+determination في ال medical laboratories، في
+
+9
+00:00:39,060 --> 00:00:44,420
+المختبرات الطبية، هذا مبدأ عمل أجهزة تستخدم في
+
+10
+00:00:44,420 --> 00:00:48,300
+معرفة التركيز أو قياس تركيز المحاليل والعينات
+
+11
+00:00:48,300 --> 00:00:53,290
+بمبدأ عمل ال calorie meter وال photometer، طيب ما
+
+12
+00:00:53,290 --> 00:00:59,010
+علاقة قياس شدة اللون أو الضوء بالتركيز؟ راح نتعرف
+
+13
+00:00:59,010 --> 00:01:04,550
+خلال ال slides القادمة، ولكن في البداية لابد من
+
+14
+00:01:04,550 --> 00:01:09,250
+تقديم بسيط نتعرف فيه ماذا يعني photometry، photo
+
+15
+00:01:09,250 --> 00:01:15,770
+حكينا معناها ضوء، متري قياس، إذن photometry معناها
+
+16
+00:01:15,770 --> 00:01:20,730
+the measurement of light، قياس الضوء، تمام؟ في عندي
+
+17
+00:01:20,730 --> 00:01:26,110
+أجهزة تسمى photometer أو spectrophotometer، هي
+
+18
+00:01:26,110 --> 00:01:30,230
+عبارة عن أجهزة تعمل على مبدأ قياس الضوء، the
+
+19
+00:01:30,230 --> 00:01:33,970
+measurement of light، و هذه الأجهزة، they are
+
+20
+00:01:33,970 --> 00:01:40,750
+instruments used to detect the measurement أو to
+
+21
+00:01:40,750 --> 00:01:45,390
+detect the concentration، و هذه الأجهزة يوجد فيها
+
+22
+00:01:45,390 --> 00:01:50,690
+أدوات أو أداة تسمى photocell أو photo multiplier
+
+23
+00:01:50,690 --> 00:01:54,940
+tube، هذه ال photocell أو ال photo multiplier tube
+
+24
+00:01:54,940 --> 00:01:59,880
+من خصائصها إنه لديها القدرة على إنها تقيس كمية
+
+25
+00:01:59,880 --> 00:02:06,240
+الضوء الواصلة إليها بعد مرورها بمحلول ملون، تمام؟
+
+26
+00:02:06,240 --> 00:02:11,490
+فإذا كان في عندي محلول ملون، و.. أو يعني reaction
+
+27
+00:02:11,490 --> 00:02:16,890
+حدث نتيجة مثلاً ال combination، الإضافة ما بين ال
+
+28
+00:02:16,890 --> 00:02:19,890
+sample وال reagent، حكينا ال reagent هو عبارة عن
+
+29
+00:02:19,890 --> 00:02:24,210
+إيش؟ هو عبارة عن محلول بيعمل لي measurable change،
+
+30
+00:02:25,160 --> 00:02:30,260
+تغير يمكن قياسه، وهي على أغلب الأحوال التغيرات ممكن تكون
+
+31
+00:02:30,260 --> 00:02:34,680
+تغير في اللون طبعاً، فإذا نتيجة reaction معين ما بين
+
+32
+00:02:34,680 --> 00:02:38,880
+ال sample وال reagent نتج colored solution، نتج
+
+33
+00:02:38,880 --> 00:02:46,320
+محلول ملون، هذا المحلول إذا مر من خلاله ضوء فإن جزء
+
+34
+00:02:46,320 --> 00:02:51,920
+من هذا الضوء اللي مر أو اصطدم بهذا المحلول، جزء من
+
+35
+00:02:51,920 --> 00:02:56,780
+هذا الضوء يتبدد ويتلاشى أو يتم امتصاصه من قبل
+
+36
+00:02:56,780 --> 00:03:03,080
+المحلول الملون، والجزء الآخر ينفذ، هذا الجزء النافذ
+
+37
+00:03:03,080 --> 00:03:08,200
+يصل إلى أداة في الجهاز اسمها photocell أو photo
+
+38
+00:03:08,200 --> 00:03:13,420
+multiplier tube، هي اللي بتقيس لي كمية الضوء اللي
+
+39
+00:03:13,420 --> 00:03:17,680
+واصلة إليها، وبنهاية بتعبر لي عن تركيز المحلول
+
+40
+00:03:17,680 --> 00:03:22,940
+الملون، تمام؟ بتعبر لي عن تركيز المحلول الملون، خلينا
+
+41
+00:03:22,940 --> 00:03:34,500
+بس لحتى نقرب الفكرة، نحكي عن هذا الموضوع، هي هنا
+
+42
+00:03:34,500 --> 00:03:41,270
+مثلاً في عندي light، ضوء، مصدر ضوء طبعاً، اصطدم بأنبوب
+
+43
+00:03:41,270 --> 00:03:45,890
+فيها محلول ملون، تمام، اصطدم بأنبوب فيها محلول ملون،
+
+44
+00:03:45,890 --> 00:03:53,230
+جزء من هذا الضوء تم امتصاصه، فقدانه أو تلاشيه عبر
+
+45
+00:03:53,230 --> 00:03:57,270
+المحلول، تمام، أو من خلال المحلول، داخل المحلول تم
+
+46
+00:03:57,270 --> 00:04:03,970
+امتصاصه، والجزء الآخر نفذ، تمام؟ هذا الجزء النافذ هو
+
+47
+00:04:03,970 --> 00:04:07,310
+الذي يصل إلى أداة ال photocell أو ال photo
+
+48
+00:04:07,310 --> 00:04:11,810
+multiplier tube في جهاز ال photo meter أو ال
+
+49
+00:04:11,810 --> 00:04:19,110
+spectrophotometer، وبيعبر لي عن تركيز المحلول، كيف
+
+50
+00:04:19,110 --> 00:04:26,140
+يعبر عن تركيز المحلول؟ ببساطة، لأنه تركيز أي محلول
+
+51
+00:04:26,140 --> 00:04:31,320
+تمام، هو عبارة عن function of its color، تركيز أي
+
+52
+00:04:31,320 --> 00:04:36,220
+محلول هو يعني بـ.. بيمكن التعبير عنه أو يمكن قياسه
+
+53
+00:04:36,220 --> 00:04:40,720
+من خلال شدة لونه، فمثلاً لو أنا حصلت على صبغة معينة
+
+54
+00:04:40,720 --> 00:04:46,020
+لنفرض أنه صبغة لونها أحمر، بلشت أخفف فيها طبعاً؟ بلشت
+
+55
+00:04:46,020 --> 00:04:50,560
+أخفف فيها، كل ما بعمل dilution أكثر أنا عم بقل ال
+
+56
+00:04:50,560 --> 00:04:55,220
+concentration عندي، وكذلك تقل شدة اللون للمحلول،
+
+57
+00:04:55,220 --> 00:05:01,860
+أعلى concentration هو أكثر شدة للون، وأقل
+
+58
+00:05:02,310 --> 00:05:05,370
+concentration أو أقل concentration هو أخف شدة
+
+59
+00:05:05,370 --> 00:05:10,470
+للون، تمام؟ إذن يتم التعبير عن تركيز المحاليل من
+
+60
+00:05:10,470 --> 00:05:17,590
+خلال لونها، وأيضاً بالتالي يمكن قياس هذه المحاليل من
+
+61
+00:05:17,590 --> 00:05:22,930
+يعني من خلال التعبير عن درجة لونها، وذلك بمرور الضوء
+
+62
+00:05:23,120 --> 00:05:27,800
+يعني كمية الضوء اللي تتلاشى في هذا المحلول، والضوء
+
+63
+00:05:27,800 --> 00:05:32,660
+اللي ينفذ عبر هذا المحلول، يعبر لي بطريقة غير مباشرة
+
+64
+00:05:32,660 --> 00:05:37,080
+عن تركيز المحلول، تمام؟ يعبر لي بطريقة غير مباشرة عن
+
+65
+00:05:37,080 --> 00:05:44,270
+تركيز المحلول، فكرة أخرى، لو أنا جبت لمبة، لمبة ضوء
+
+66
+00:05:44,270 --> 00:05:49,570
+وحطيت أمامها قطعة زجاجية ملونة، هل كل الضوء اللي
+
+67
+00:05:49,570 --> 00:05:53,230
+موجود في ال light source، في المصدر، في اللمبة،
+
+68
+00:05:53,230 --> 00:05:56,310
+سينفذ إلى الجهة الأخرى من القطعة الزجاجية؟ بالطبع
+
+69
+00:05:56,310 --> 00:06:00,150
+لا، يعني لا، جزء من هذا الضوء سيتلاشى عبر القطعة
+
+70
+00:06:00,150 --> 00:06:05,950
+الزجاجية، وجزء آخر سينفذ، فبالتالي لو كل ما كان لون
+
+71
+00:06:05,950 --> 00:06:11,820
+الزجاج داكن أكثر، غامق أكثر، فبالتالي هيحجب كمية
+
+72
+00:06:11,820 --> 00:06:16,180
+الضوء أكثر، تمام؟ وكمية الضوء اللي تنفذ عبر هذه
+
+73
+00:06:16,180 --> 00:06:20,660
+القطعة الزجاجية هيكون أقل، تمام؟ فهذه هي فكرة
+
+74
+00:06:20,660 --> 00:06:27,280
+القياس.. التركيز تبع لشدة لونها، و لمرور الضوء من
+
+75
+00:06:27,280 --> 00:06:27,780
+خلالها
+
+76
+00:06:35,350 --> 00:06:39,630
+طيب لنتحدث عن characteristics of light، خواص الضوء
+
+77
+00:06:39,630 --> 00:06:44,230
+الضوء هو عبارة عن electromagnetic energy that
+
+78
+00:06:44,230 --> 00:06:48,830
+travels in waves، هو عبارة عن طاقة كهرومغناطيسية
+
+79
+00:06:48,830 --> 00:06:53,930
+تسير في موجات، ومنها تسير في موجات، إذا الطول
+
+80
+00:06:53,930 --> 00:06:58,210
+الموجي لهذا الضوء هو عبارة عن the wavelength of
+
+81
+00:06:58,210 --> 00:07:03,410
+light is the distance، هو عبارة عن المسافة ما بين
+
+82
+00:07:03,410 --> 00:07:08,390
+two peaks، two peaks يعني ما بين قمتين متتاليتين أو
+
+83
+00:07:08,390 --> 00:07:13,350
+قاعين متتاليين، أعلى نقطة في القمة بنسميها peak، وأقل
+
+84
+00:07:13,350 --> 00:07:19,110
+نقطة في القاع أيضاً بنسميها peak، إذا بما أنه الطول
+
+85
+00:07:19,110 --> 00:07:22,230
+الموجي، إذا بدنا نحكي عنه بنعبر عنه بالعربي هو
+
+86
+00:07:22,230 --> 00:07:27,940
+المسافة ما بين قمتين متتاليتين أو قاعين متتاليين، و
+
+87
+00:07:27,940 --> 00:07:31,020
+أعلى نقطة في القمة بنسميها peak، وأعلى نقطة أو
+
+88
+00:07:31,020 --> 00:07:35,740
+أدنى نقطة في القاع بنسميه أيضاً peak، إذا هو في
+
+89
+00:07:35,740 --> 00:07:39,540
+اللغة الإنجليزية نعرفه the distance between two
+
+90
+00:07:39,540 --> 00:07:44,920
+peaks of the light wave، هذا الطول الموجي يتناسب
+
+91
+00:07:44,920 --> 00:07:50,620
+عكسياً مع طاقة الضوء، إذا إذا كانت عندي ضوء بطول
+
+92
+00:07:50,620 --> 00:07:56,500
+موجي كبير، ستكون طاقة هذا الضوء قليلة، والعكس صحيح،
+
+93
+00:07:56,500 --> 00:08:01,300
+إذا كان الطول الموجي قليل، إذا طاقة هذا الضوء ستكون
+
+94
+00:08:01,300 --> 00:08:05,780
+عالية جداً، تمام؟ العلاقة عكسية ما بين الطول الموجي
+
+95
+00:08:05,780 --> 00:08:11,200
+وطاقة الضوء، طيب أنا في عندي أنواع من الضوء، في ضوء
+
+96
+00:08:11,200 --> 00:08:15,040
+مرئي، في ضوء أشعة فوق بنفسجية، أشعة تحت حمراء،
+
+97
+00:08:15,040 --> 00:08:18,660
+gamma، X-ray، radio waves، في عندي أنواع كثيرة من
+
+98
+00:08:18,660 --> 00:08:24,220
+الأشعة الضوئية، طيب لو كنا نتحدث عن الأشعة المرئية
+
+99
+00:08:24,220 --> 00:08:27,780
+التي يمكن رؤيتها بالعين، تمام؟ the visible region
+
+100
+00:08:27,780 --> 00:08:31,860
+the visible region هي الأشعة المرئية، تمام؟ هذه
+
+101
+00:08:31,860 --> 00:08:37,050
+الأشعة المرئية، بيحكي لي إنه the color of light is
+
+102
+00:08:37,050 --> 00:08:43,780
+a function of its wavelength، لون الضوء عبارة عن دالة
+
+103
+00:08:43,780 --> 00:08:48,700
+أو يعبر عنه بالطول الموجي طبعاً، اللون اللي.. اللون
+
+104
+00:08:48,700 --> 00:08:53,160
+اللي الضوء يعبر عنه بالطول الموجي، هذا الطول الموجي
+
+105
+00:08:53,160 --> 00:08:58,540
+يزداد من اللون ال violet اللي هو أول لون في مرحلة
+
+106
+00:08:58,540 --> 00:09:03,080
+ال visible، وبيظل يزيد باتجاه ال red color اللي هو
+
+107
+00:09:03,080 --> 00:09:06,700
+آخر لون في مرحلة ال visible، كمان شوية راح نستعرض
+
+108
+00:09:06,700 --> 00:09:11,100
+جدول، وراح نفهم شو يعني ال visible region، تمام؟
+
+109
+00:09:11,100 --> 00:09:14,660
+خلينا بس ننتقل إلى النقطة الأخيرة اللي بتحكي لي
+
+110
+00:09:14,660 --> 00:09:18,300
+objects that appear colored absorb light at
+
+111
+00:09:18,300 --> 00:09:21,840
+particular wavelengths and reflect the other parts
+
+112
+00:09:21,840 --> 00:09:24,880
+of the visible spectrum resulting in many shades
+
+113
+00:09:24,880 --> 00:09:29,780
+of color، إيش معنى هذه الجملة؟ حكينا إنه المحلول
+
+114
+00:09:29,780 --> 00:09:35,580
+اللي يبدو ملوناً، تمام، colored solution absorb light،
+
+115
+00:09:35,580 --> 00:09:41,120
+يمتص الضوء على طول موجي معين، ويعكس أو ينفذ من
+
+116
+00:09:41,120 --> 00:09:45,320
+خلاله الضوء الآخر، أو ما تبقى، the other parts، ما
+
+117
+00:09:45,320 --> 00:09:53,140
+تبقى من هذا الضوء، ومثل ما عرضنا من شوية في الصورة،
+
+118
+00:09:53,140 --> 00:09:57,280
+مبدأ عمل هذا الجهاز بيحكي لي إنه if a substance
+
+119
+00:09:57,280 --> 00:10:02,000
+تمام؟ if a substance can be converted to a soluble
+
+120
+00:10:02,000 --> 00:10:06,440
+and colored material، إذا كان في عندي محلول أو مادة
+
+121
+00:10:06,440 --> 00:10:11,020
+أو نتيجة تفاعل هي عبارة عن سولابل، ذائبة وملونة،
+
+122
+00:10:11,020 --> 00:10:16,000
+فممكن أنا أعمل determination لل concentration لهذا
+
+123
+00:10:16,000 --> 00:10:19,440
+المحلول، its concentration may be determined، ممكن
+
+124
+00:10:19,440 --> 00:10:25,760
+أعرف التركيز من خلال كمية اللون اللي موجودة في
+
+125
+00:10:25,760 --> 00:10:30,180
+المحلول، وهذا الكلام أيضاً استعرضناه في صورة، حكينا
+
+126
+00:10:30,180 --> 00:10:35,180
+إنه كل ما زاد التركيز، بتزيد شدة اللون، طبعاً كل ما
+
+127
+00:10:35,180 --> 00:10:40,880
+زاد التركيز بتزيد شدة اللون للمحلول، لو جبنا صبغة، و
+
+128
+00:10:40,880 --> 00:10:45,120
+الصبغة هذه لونها أحمر مثلاً، تمام؟ وبلشت أخفف فيها،
+
+129
+00:10:46,330 --> 00:10:50,510
+أخفف فيها باستخدام مثلاً distilled water، ماء طبعاً،
+
+130
+00:10:50,510 --> 00:10:54,650
+أضيف عليها ماء أكثر فأكثر فأكثر، هلاحظ أنه تركيزها
+
+131
+00:10:54,650 --> 00:11:00,330
+بيقل مع ال dilution، كل ما خففت أكثر التركيز بيقل،
+
+132
+00:11:00,330 --> 00:11:08,410
+وبالتالي اللون بيقل طبعاً، فإذا دائماً التركيز
+
+133
+00:11:08,410 --> 00:11:12,710
+ممكن أن أعمله determination من خلال شدة اللون لهذا
+
+134
+00:11:12,710 --> 00:11:16,550
+المحلول، The greatest sensitivity is obtained when
+
+135
+00:11:16,550 --> 00:11:19,370
+the light permitted to pass through the solution
+
+136
+00:11:19,370 --> 00:11:23,450
+is of its particular wavelength، طبعاً عشان تكون هذه
+
+137
+00:11:23,450 --> 00:11:32,430
+الطريقة حساسة جداً يعني لقياس التركيز، لابد يا بنات
+
+138
+00:11:32,430 --> 00:11:37,590
+أنه أنا أستخدم الطول الموجي اللي يحدث عنده أقصى
+
+139
+00:11:37,590 --> 00:11:43,670
+كمية امتصاص، أقصى كمية امتصاص، ماذا يعني هذا الكلام؟
+
+140
+00:11:43,670 --> 00:11:47,690
+خلينا بس نشوف الصورة اللي هنا، هلأ أنا كان في عندي
+
+141
+00:11:47,690 --> 00:11:52,510
+light source، تمام؟ لمبة بتعطيني أطوال موجية مختلفة،
+
+142
+00:11:52,510 --> 00:11:57,870
+هذه الأطوال الموجية المختلفة، لابد من إنه أنا أختار
+
+143
+00:11:57,870 --> 00:12:02,770
+طول موجي واحد فقط، إنه اللي يعبر من خلال الأنبوبة،
+
+144
+00:12:02,770 --> 00:12:08,350
+وجزء منه يتم امتصاصه، والجزء الآخر ينفذ، يروح على
+
+145
+00:12:08,350 --> 00:12:11,710
+ال detector، ال detector يقيس كمية الضوء اللي وصلته،
+
+146
+00:12:11,920 --> 00:12:16,930
+يعرف منها تركيز العينة، طبعاً، طيب على أي أساس هذا
+
+147
+00:12:16,930 --> 00:12:20,450
+الطول الموجي الواحد اللي لازم أختاره من عدة أطوال
+
+148
+00:12:20,450 --> 00:12:25,070
+موجية موجودة في ال light source؟ بنختاره طبعاً لأنه
+
+149
+00:12:25,070 --> 00:12:31,610
+لازم يكون المحلول يمتصه بأقصى امتصاص، إذا الجزء
+
+150
+00:12:31,610 --> 00:12:35,870
+النافذ يكون أقل كمية يمكن نفادها عبر هذا المحلول،
+
+151
+00:12:35,870 --> 00:12:39,730
+مدام ممتص أكبر كمية ممكنة من المحلول، إذا اللي
+
+152
+00:12:39,730 --> 00:12:45,860
+نافذ هنا هيكون أقل كمية من الضوء، إذن بما إنه امتص
+
+153
+00:12:45,860 --> 00:12:50,860
+أكبر كمية من الضوء، the maximum absorbance of
+
+154
+00:12:50,860 --> 00:12:55,320
+light، تمام؟ معناها اللي نفذ هو أقل كمية يمكن أن
+
+155
+00:12:55,320 --> 00:12:59,600
+تنفذ من هذا الضوء، تمام؟ إذن لابد من إنه اختيار
+
+156
+00:12:59,600 --> 00:13:05,740
+طول موجي واحد ومحدد، اللي خواصه إنه عنده المحلول
+
+157
+00:13:05,740 --> 00:13:10,040
+بيقوم بـ the maximum absorbance، فبالتالي هيكون the
+
+158
+00:13:10,040 --> 00:13:14,630
+least أو the lowest transmitted light، طبعاً the
+
+159
+00:13:14,630 --> 00:13:23,410
+lowest transmitted light، طيب بالنسبة لأنواع
+
+160
+00:13:23,410 --> 00:13:27,430
+الطاقة الضوئية، إحنا حكينا إنه في عندي أكثر من نوع
+
+161
+00:13:27,430 --> 00:13:32,330
+من ال.. الأشعة الضوئية، gamma، X-ray، ultraviolet،
+
+162
+00:13:32,330 --> 00:13:37,090
+visible، infrared، radio waves، طبعاً هنا بيكون أقل
+
+163
+00:13:37,090 --> 00:13:43,200
+طول موجي، طبعاً، وهنا أعلى طول موجي، إذا هنا هيكون
+
+164
+00:13:43,200 --> 00:13:48,760
+أكثرها طاقة، وهنا هيكون أقلها طاقة، ليش؟ لأن
+
+165
+00:13:48,760 --> 00:13:57,020
+العلاقة عكسية ما بين الطول الموجي والطاقة،
+
+166
+00:13:57,670 --> 00:14:02,910
+تمام؟ إذا زادت الطاقة يقل الطول الموجي، هيك بهذا
+
+167
+00:14:02,910 --> 00:14:06,310
+الاتجاه بيزيد الطول الموجي، هذا أقلها طول موجي، وهذا
+
+168
+00:14:06,310 --> 00:14:10,950
+أكثرها طول موجي، هيك بتزيد الطاقة، هذه أقلها
+
+169
+00:14:10,950 --> 00:14:15,950
+طاقة، وهذه أعلاها طاقة من نوع الأشعة، تمام؟ إحنا
+
+170
+00:14:15,950 --> 00:14:19,700
+حكينا في ال visible، تمام؟ هذه المنطقة تبدأ
+
+171
+00:14:19,700 --> 00:14:23,000
+بال violet، لأنه أصلاً المنطقة اللي فوقها ultraviolet،
+
+172
+00:14:23,000 --> 00:14:27,120
+ما فوق البنفسجية، فبتبدأ بال violet وبتنتهي بال red،
+
+173
+00:14:27,120 --> 00:14:30,560
+لأن المنطقة اللي بعدها infrared، تحت حمراء، فتدرج
+
+174
+00:14:30,560 --> 00:14:33,640
+الألوان بيكون بدايته في منطقة ال visible، بدايته
+
+175
+00:14:33,
+
+223
+00:18:09,800 --> 00:18:14,280
+إنه المحلول يمتصه بأقصى امتصاص طبعا، مش أنا
+
+224
+00:18:14,280 --> 00:18:18,180
+ما بدي range  لقاعدة لعديد من الأطوار الموجية  تُختارِق
+
+225
+00:18:18,180 --> 00:18:22,820
+المحلول، لأ أنا بدي طول موجي واحد فقط ليختارق
+
+226
+00:18:22,820 --> 00:18:27,820
+المحلول. تمام؟ فإن جزءا من هذا الضوء يتم امتصاصه، راح
+
+227
+00:18:27,820 --> 00:18:32,720
+يصير له absorbed، نقول يتلاشى أو يتم امتصاصه من
+
+228
+00:18:32,720 --> 00:18:37,920
+داخل المحلول، تمام؟ والـ rest، الباقي، ما تبقى
+
+229
+00:18:37,920 --> 00:18:42,810
+من هذا الضوء، تمام؟  إيش راح يصير له؟ راح يصير له
+
+230
+00:18:42,810 --> 00:18:47,690
+transmitted، تمام؟ ينفذ، transmitted يعني ينفذ، و
+
+231
+00:18:47,690 --> 00:18:51,090
+وين راح يروح؟ راح يروح على الـ detector اللي موجود
+
+232
+00:18:51,090 --> 00:18:55,570
+في الجهاز. إيش هو هذا الـ detector؟ هو الـ photo cell
+
+233
+00:18:55,570 --> 00:19:00,390
+أو الـ photomultiplier tube اللي موجودة في الجهاز
+
+234
+00:19:00,390 --> 00:19:04,850
+واللي ذكرناها بأول slide. طبعا، طيب لو جينا حسبنا
+
+235
+00:19:04,850 --> 00:19:07,590
+نسبة الـ transmittance، النسبة المئوية للـ
+
+236
+00:19:07,590 --> 00:19:11,510
+transmittance.  نفترض أن الضوء اللي جاي من الـ
+
+237
+00:19:11,510 --> 00:19:15,870
+light source هو الـ I₀، شدة الضوء الابتدائية I₀
+
+238
+00:19:15,870 --> 00:19:22,850
+ونفترض أن الضوء اللي نفذ من خلال الـ tube وذهب
+
+239
+00:19:22,850 --> 00:19:26,850
+إلى الـ detector هو الـ I<sub>T</sub>، شدة الضوء للـ
+
+240
+00:19:26,850 --> 00:19:31,130
+transmittance اللي هو النافذ. طيب معناها الـ percent
+
+241
+00:19:31,130 --> 00:19:36,350
+of transmittance هي الـ I<sub>T</sub> على الـ I₀ × ١٠٠ في
+
+242
+00:19:36,350 --> 00:19:39,410
+المئة، زي قانون النسبة المئوية العادي اللي إحنا
+
+243
+00:19:39,410 --> 00:19:43,090
+بنستخدمه بحياتنا اليومية، الجزء على الكل × ١٠٠ في
+
+244
+00:19:43,090 --> 00:19:47,570
+المئة، هذه نسبة الـ transmittance. طيب، هل هذا ما
+
+245
+00:19:47,570 --> 00:19:53,170
+قاله بير فقط؟ لأ، بير حكى لنا كمان إنه if the
+
+246
+00:19:53,170 --> 00:19:56,630
+concentration of solution is increased the I
+
+247
+00:19:56,630 --> 00:20:02,470
+transmitted will decrease and the transmitted
+
+248
+00:20:02,470 --> 00:20:06,170
+percentage is decreased. إيش معنى هذا الكلام؟ خلينا
+
+249
+00:20:06,170 --> 00:20:10,870
+نشوف لو كان في عندي… لو زاد تركيز هذا المحلول
+
+250
+00:20:10,870 --> 00:20:15,590
+إذن هتزيد شدة لونه، تمام؟ إذن هتزيد العتامة لهذا
+
+251
+00:20:15,590 --> 00:20:20,960
+المحلول، تمام؟ زادت شدة لونه، اتخيلي هل… الضوء
+
+252
+00:20:20,960 --> 00:20:26,780
+سينفذ بشكل كبير من خلاله؟ بالعكس، إذا كل ما زادت
+
+253
+00:20:26,780 --> 00:20:32,200
+شدة اللون، تمام؟ أو كل ما زاد التركيز، هيزيد شدة
+
+254
+00:20:32,200 --> 00:20:41,540
+اللون، إذن هذا المحلول راح يمتص أو راح يعني يتلاشى
+
+255
+00:20:41,540 --> 00:20:47,860
+من خلاله أو يتلاشى فيه أكثر، تمام؟ كمية الضوء الممتص
+
+256
+00:20:47,860 --> 00:20:53,240
+من خلاله أكثر، الـ absorbance أعلى، تمام؟ إذن كمية
+
+257
+00:20:53,240 --> 00:20:58,020
+الضوء النافذة هتكون أقل. إذن هناك علاقة عكسية ما
+
+258
+00:20:58,020 --> 00:21:01,940
+بين الـ I<sub>T</sub> ونسبة الـ transmittance والتركيز. كل ما
+
+259
+00:21:01,940 --> 00:21:06,020
+زاد التركيز، كل ما زاد اللون، الضوء اللي هيمتص أعلى،
+
+260
+00:21:06,020 --> 00:21:10,930
+إذن الـ transmittance هيكون أقل، وهذا هو قانون
+
+261
+00:21:10,930 --> 00:21:16,010
+بير، بينص… هو بير درس علاقة التركيز بالـ
+
+262
+00:21:16,010 --> 00:21:19,590
+transmitted light، وعبّر عنه بهذه العلاقة. حتى إن
+
+263
+00:21:19,590 --> 00:21:24,110
+العلاقة عكسية طبعا، العلاقة عكسية ما بين مين ومين؟ ما بين التركيز والـ
+
+264
+00:21:24,110 --> 00:21:29,230
+transmittance. حكى إنه كل
+
+265
+00:21:29,230 --> 00:21:34,330
+ما زاد التركيز، بيصير في عندي بتقل شدة الضوء
+
+266
+00:21:34,330 --> 00:21:37,170
+المنبعث اللي هو الـ transmitted، اه الـ I<sub>transmitted</sub>
+
+267
+00:21:37,170 --> 00:21:41,130
+أو الـ I<sub>T</sub>، وبالتالي نسبة الضوء المنبعث بالنسبة للكل
+
+268
+00:21:41,130 --> 00:21:45,730
+هتكون أيضا قليلة. the relationship between the
+
+269
+00:21:45,730 --> 00:21:48,410
+concentration and transmitted is not linear.
+
+270
+00:21:48,410 --> 00:21:52,090
+العلاقة مش linear ما بين التركيز والـ
+
+271
+00:21:52,090 --> 00:21:55,890
+transmittance. إحنا شوية حكينا العلاقة عكسية، وكلمة
+
+272
+00:21:55,890 --> 00:21:59,590
+linear يعني علاقة خطية أو علاقة طردية. إذا the
+
+273
+00:21:59,590 --> 00:22:03,790
+relation between concentration and transmittance
+
+274
+00:22:03,790 --> 00:22:07,410
+is not linear، ليست خطية وليست طردية، إنما they are
+
+275
+00:22:07,410 --> 00:22:12,530
+inversely proportional، هم يتناسبوا تناسب عكسي.
+
+276
+00:22:12,530 --> 00:22:20,550
+inversely. طيب العلاقة هاي بيوضحها هذا الـ curve.
+
+277
+00:22:20,550 --> 00:22:25,330
+هلق هي هنا على الـ y-axis نسبة الـ transmittance،
+
+278
+00:22:25,330 --> 00:22:30,630
+نسبة الضوء المنبعث أو النافذ، تمام؟ وعلى الـ x-axis
+
+279
+00:22:30,630 --> 00:22:35,750
+في عندي التركيز، الـ concentration. طيب خلينا نشوف،
+
+280
+00:22:35,750 --> 00:22:38,890
+هاي أقل تركيز هنا، هتكون تمام؟ بالقرب من الـ zero،
+
+281
+00:22:38,890 --> 00:22:43,770
+أقل تركيز، هذا الأقل من التركيز، إيش؟ إلها… إلها
+
+282
+00:22:43,770 --> 00:22:51,020
+أعلى transmittance، تمام؟ وأعلى تراكيز، إيش إلها؟
+
+283
+00:22:51,020 --> 00:22:54,820
+إلها أقل transmittance، تمام؟ إلها أقل
+
+284
+00:22:54,820 --> 00:22:58,620
+transmittance. إذن العلاقة عكسية، ولو جينا نربطها
+
+285
+00:22:58,620 --> 00:23:02,380
+منطقيا… لو نيجي… لو نيجي نربطها منطقيا، نرجع
+
+286
+00:23:02,380 --> 00:23:06,570
+للصورة اللي فيها تدرج الألوان، هذه. أعلى تركيز يعني
+
+287
+00:23:06,570 --> 00:23:10,630
+أعلى شدة لون، لو مرّ الضوء، كمية قليلة اللي هتنفذ من
+
+288
+00:23:10,630 --> 00:23:15,870
+خلاله، تمام؟ لكن أقل تركيز يعني أقل شدة لون، إذن
+
+289
+00:23:15,870 --> 00:23:20,170
+مرّ الضوء من خلاله، كمية كبيرة من الضوء راح تنفذ،
+
+290
+00:23:20,170 --> 00:23:24,730
+تمام؟ لو أنا جيبت لمبة وحطيت أمامها زجاجة شفافة، هذه
+
+291
+00:23:24,730 --> 00:23:29,050
+الزجاجة الشفافة راح تنفذ لكمية ضوء كبيرة. طيب لو
+
+292
+00:23:29,050 --> 00:23:33,850
+حطيت هذه الزجاجة بلون أبيض، هينفذ كمية ضوء أقل. طيب
+
+293
+00:23:33,850 --> 00:23:39,490
+لو حطيت هذه الزجاجة اللي أمام اللمبة بلون رمادي،
+
+294
+00:23:39,490 --> 00:23:43,630
+إذن هتحجب ضوء أكثر، وكمية ضوء النافذ هتكون
+
+295
+00:23:43,630 --> 00:23:49,200
+أقل. طيب لو حطيتها بلون أسود، هيحجب الضوء أكثر فأكثر،
+
+296
+00:23:49,200 --> 00:23:53,440
+النافذ من خلال قطعة الزجاج. إذن هذه هي العلاقة ما
+
+297
+00:23:53,440 --> 00:23:55,980
+بين شدة اللون، كل ما زادت شدة اللون يعني زاد
+
+298
+00:23:55,980 --> 00:24:00,840
+التركيز، كمية الضوء النافذ هتكون قليلة، هتكون كمية
+
+299
+00:24:00,840 --> 00:24:04,500
+الضوء اللي نافذ أو المنبعث عبر الـ tube، الـ
+
+300
+00:24:04,500 --> 00:24:07,960
+transmitted light هيكون إيش؟ قليل.
+
+301
+00:24:10,670 --> 00:24:16,530
+طيب، ماذا تصبح العلاقة linear، طردية، إذا أنا مثّلت
+
+302
+00:24:16,530 --> 00:24:21,890
+على الـ Y axis سالب log T، مش نسبة الـ T، سالب
+
+303
+00:24:21,890 --> 00:24:26,450
+log T، وهذا سالب log T بنعبر عنه بالـ A اللي
+
+304
+00:24:26,450 --> 00:24:33,690
+هو absorbance، تمام؟ absorbance. طيب إحنا طبعا كمية
+
+305
+00:24:33,690 --> 00:24:37,990
+الضوء الممتص، كل ما زاد التركيز بيزيد اللون، إذن
+
+306
+00:24:37,990 --> 00:24:43,630
+هيزيد الامتصاص. فإذا العلاقة linear وطردية ما بين
+
+307
+00:24:43,630 --> 00:24:48,990
+التركيز والامتصاص، والعلاقة عكسية، inversely، not
+
+308
+00:24:48,990 --> 00:24:55,940
+linear ما بين التركيز والـ transmittance. طيب بير
+
+309
+00:24:55,940 --> 00:25:01,140
+ولا مبرت، لا، لامبرت تعاون مع بير ووضعوا قانون،
+
+310
+00:25:01,140 --> 00:25:07,260
+آخر هذا القانون سموه A = εbc. إيش يعني A = εbc؟ خلينا أولا ناخد نص القانون، وبعدين
+
+311
+00:25:07,260 --> 00:25:11,340
+ناخد اللي هو التعبير الحسابي أو الرياضي عن هذا
+
+312
+00:25:11,340 --> 00:25:16,000
+القانون. Beer Lambert قانونهم بينص على أنه
+
+313
+00:25:16,000 --> 00:25:21,300
+الـ absorbance of solution is directly proportional،
+
+314
+00:25:21,300 --> 00:25:26,720
+يعني العلاقة linear، directly proportional، يتناسب
+
+315
+00:25:26,720 --> 00:25:30,000
+طرديا، العلاقة linear with، with the
+
+316
+00:25:30,000 --> 00:25:33,400
+concentration of substance، تمام؟ إذن بير لحاله درس
+
+317
+00:25:33,400 --> 00:25:39,940
+علاقة الـ transmittance مع الـ concentration، وحكى
+
+318
+00:25:39,940 --> 00:25:43,980
+إنه العلاقة عكسية. بير ولامبرت درسوا التركيز والـ
+
+319
+00:25:43,980 --> 00:25:50,340
+…الـ concentration مع الـ absorbance، وحكوا إنه
+
+320
+00:25:50,340 --> 00:25:53,880
+…العلاقة طردية، وهذا الكلام صار بديهي، وصرنا بنعرفه.
+
+321
+00:25:59,250 --> 00:26:02,750
+طيب التعبير الرياضي، عبروا عن الـ A الكبيرة بالـ
+
+322
+00:26:02,750 --> 00:26:06,190
+absorbance، يعني هلق بدهم يدرسوا الـ absorbance طبعا،
+
+323
+00:26:06,190 --> 00:26:12,970
+فلازم يصيغوا له تعبير رياضي:  -log T،  فحكوا إن
+
+324
+00:26:12,970 --> 00:26:20,070
+هو يساوي εbc، تمام؟ طيب ε هي عبارة عن
+
+325
+00:26:20,070 --> 00:26:25,310
+المولار absorptivity coefficient، والـ b هو عبارة
+
+326
+00:26:25,310 --> 00:26:30,550
+عن مسار الضوء ما بين الـ light source والعينة،
+
+327
+00:26:30,740 --> 00:26:35,500
+الضوء اللي بيمرّ من خلاله في الجهاز، مسار الضوء أو
+
+328
+00:26:35,500 --> 00:26:39,040
+المسافة اللي بيمرّها الضوء داخل الجهاز ما بين الـ
+
+329
+00:26:39,040 --> 00:26:42,280
+light source، اللمبة، طبعا، الضوء اللي بتزودني أطوار
+
+330
+00:26:42,280 --> 00:26:47,040
+موجية عديدة، وما بين الـ…الـ…المكان اللي أنا
+
+331
+00:26:47,040 --> 00:26:50,020
+بحط فيه الـ cuvette، الأنبوبة اللي فيها المحلول
+
+332
+00:26:50,020 --> 00:26:55,860
+الملون المراد قياس تركيزه. تمام؟ هذه هي b، إلها علاقة
+
+333
+00:26:55,860 --> 00:26:59,960
+بالجهاز وليس…يعني أنا ما بلعب فيها، إذن هي ثابتة،
+
+334
+00:26:59,960 --> 00:27:05,120
+…ثابتة، إلها علاقة بالجهاز المستخدم.  ε إلها علاقة
+
+335
+00:27:05,120 --> 00:27:07,380
+بنوع المادة المراد قياسها.
+
+336
+00:27:10,330 --> 00:27:15,310
+إحنا حكينا في البداية إنه إذا أنا بدي أقيص تركيز
+
+337
+00:27:15,310 --> 00:27:20,590
+مجهول في عينة، لابد إني أقيصه بدلالة معلوم، ألا وهو
+
+338
+00:27:20,590 --> 00:27:26,350
+الـ standard، تمام؟ ومثلا لو أنا بدي أشتغل أو أبدأ
+
+339
+00:27:26,350 --> 00:27:32,060
+أعمل فحص السكري، أعمل فحص السكر، حكينا إنه إذا
+
+340
+00:27:32,060 --> 00:27:35,200
+بتتذكروا محاضرة الـ dilution، رسمت لكم two tubes،
+
+341
+00:27:35,200 --> 00:27:39,800
+حكيت لكم أنا بجيب عينة أو tube فيها العينة، والـ
+
+342
+00:27:39,800 --> 00:27:43,580
+reagent، متفاعلين طبعا، الـ reagent بضيفه أو بضيف
+
+343
+00:27:43,580 --> 00:27:48,080
+العينة للـ reagent، فيحدث تغيير، تغير في اللون، وأيضا
+
+344
+00:27:48,080 --> 00:27:51,440
+بضيف الـ standard للـ reagent، ويحدث تغيير في اللون.
+
+345
+00:27:51,550 --> 00:27:55,830
+إذا كان تغير اللون الحادث في العينة نصف التغير
+
+346
+00:27:55,830 --> 00:27:58,570
+الحدث في الـ standard، إذن تركيزه نصف تركيز الـ
+
+347
+00:27:58,570 --> 00:28:03,430
+standard. وإذا كان التغير الحادث في tube العينة ضعف،
+
+348
+00:28:03,430 --> 00:28:11,420
+إذن التركيز ضعف تركيز الـ standard. وهذا الكلام الـ
+
+349
+00:28:11,420 --> 00:28:15,880
+…النظري اللي إحنا بنحكي ل…لحتى نوصل لفكرة،
+
+350
+00:28:15,880 --> 00:28:20,220
+طب الكلام العملي أو الكلام العلمي، ما…إيش
+
+351
+00:28:20,220 --> 00:28:25,620
+يعني؟ شوفوا معايا لو أنا ضفت على reaction عينة،
+
+352
+00:28:25,620 --> 00:28:29,950
+طبعا عشان أ…أقيّس نسبة السكر في الدم، بيتفاعل الـ
+
+353
+00:28:29,950 --> 00:28:34,550
+reagent مع السكر ويعطيني measurable change، تغير،
+
+354
+00:28:34,550 --> 00:28:39,190
+يمكن قياسه، تغير في اللون. فمثلا ممكن يتحول اللون
+
+355
+00:28:39,190 --> 00:28:43,150
+المحلول من colorless، من عديم اللون إلى لون pink،
+
+356
+00:28:43,150 --> 00:28:47,470
+ينتج لي مادة، واللون هذه…المادة هاي لونها pink،
+
+357
+00:28:47,470 --> 00:28:51,810
+تمام؟ فهذه هي اللي بدي أقيّس ال…ال…الـ
+
+358
+00:28:51,810 --> 00:28:55,710
+absorbance تبعها، هي اللي أنا بدي أمرر ضوء من
+
+359
+00:28:55,710 --> 00:29:01,050
+خلالها وأشوف كم هي تمتص من الضوء، وكم تنفذ،
+
+360
+00:29:01,050 --> 00:29:05,890
+عشان يعبر لي عن كمية الضوء الممتص أو كمية الضوء
+
+361
+00:29:05,890 --> 00:29:08,810
+النافذ عن تركيزها، عن شدة لونها.
+
+362
+00:29:16,970 --> 00:29:22,830
+طبعا يكون في عندي reagent، هذا الـ reagent أنا بضيفه
+
+363
+00:29:22,830 --> 00:29:28,730
+لـ standard، والـ standard بيكون محلول من نفس نوع
+
+364
+00:29:28,730 --> 00:29:32,770
+الفحص، يعني أنا بشتغل فحص سكر، لازم يكون محلول سكري
+
+365
+00:29:32,770 --> 00:29:36,450
+معلوم التركيز. بشتغل فحص protein، لازم يكون محلولي
+
+366
+00:29:36,450 --> 00:29:40,170
+protein معلوم التركيز. بشتغل فحص دهون، لازم يكون
+
+367
+00:29:40,170 --> 00:29:44,910
+محلول دهون معلوم التركيز. ما بنفع إنه مثلا أكون
+
+368
+00:29:44,910 --> 00:29:49,170
+بشتغل فحص سكر للعينة وفحص مثلا…أو دهون للـ
+
+369
+00:29:49,170 --> 00:29:54,370
+standard، غلط، غلط. لازم المادة اللي ناتجها الملون
+
+370
+00:29:54,370 --> 00:29:59,150
+هتكون نفسها، تمام؟ المادة الملونة والناتج هتكون
+
+371
+00:29:59,150 --> 00:30:04,050
+نفسها، علشان يكون لها نفس الـ molar absorptivity
+
+372
+00:30:04,050 --> 00:30:08,810
+coefficient، تمام؟ طيب، طب لو أنا جيت…طبقت هذا
+
+373
+00:30:08,810 --> 00:30:15,230
+القانون مرة لعينة مجهولة ومرة للـ standard، فبيصير
+
+374
+00:30:15,230 --> 00:30:20,190
+مثلا عندي الـ absorbance للـ X اللي هو المجهول يساوي
+
+375
+00:30:20,190 --> 00:30:25,050
+εbc، تركيز الـ X اللي هو إحنا ما بنعرفه. الـ
+
+376
+00:30:25,050 --> 00:30:27,530
+absorbance للـ standard، طبعا الـ absorbance هذه
+
+377
+00:30:27,530 --> 00:30:30,550
+قراءات بيعطيني إياها الجهاز، يعني هو اللي بيقيّس كمية الـ
+
+378
+00:30:30,550 --> 00:30:33,730
+absorbance، كمية الضوء المتلاشية، بيصل للـ
+
+379
+00:30:33,730 --> 00:30:38,030
+transmittance على الـ detector وبيطرحها من ال…الـ
+
+380
+00:30:38,030 --> 00:30:42,150
+ضوء، كمية الضوء الأساسية اللي جاية من الـ source، من
+
+381
+00:30:42,150 --> 00:30:47,030
+المصدر وبيعرّفوا كمية الـ absorbance، تمام؟ طيب فالـ
+
+382
+00:30:47,030 --> 00:30:50,590
+absorbance للـ standard يساوي εbc، تركيز الـ
+
+383
+00:30:50,590 --> 00:30:53,870
+concentration للـ standard. طب لو أنا جيت قسمت الـ
+
+384
+00:30:53,870 --> 00:30:57,150
+absorbance للـ X على الـ absorbance للـ
+
+385
+00:30:57,150 --> 00:31:02,830
+standard، جيت قسمتُهم على بعض مثل هيك، absorbance للـ X
+
+386
+00:31:02,830 --> 00:31:06,850
+على absorbance للـ standard. طيب مش إحنا حكينا إنه
+
+387
+00:31:06,850 --> 00:31:11,110
+ال…ال…ال…ال…ال…الـ standard وال…
+
+388
+00:31:11,110 --> 00:31:15,850
+ال…الـ sample لازم يكونوا من نفس النوع، وينتج لي
+
+389
+00:31:15,850 --> 00:31:20,450
+بعد التفاعل نفس المادة، وهيكون لها نفس الـ ε، تمام؟
+
+390
+00:31:20,450 --> 00:31:24,070
+اللي هو
+
+445
+00:35:32,810 --> 00:35:37,680
+اللي لونه pink لو أنا استخدمت product different
+
+446
+00:35:37,680 --> 00:35:41,100
+مثلا يعني protein standard محلول ال
+
+447
+00:35:41,100 --> 00:35:45,100
+protein معلوم التركيز وأضفته لهذا ال standard
+
+448
+00:35:45,100 --> 00:35:48,620
+إلى reagent السكر
+
+449
+00:35:50,560 --> 00:35:54,380
+معناها ال protein هذا ممكن يتفاعل، وممكن
+
+450
+00:35:54,380 --> 00:35:58,640
+لا يتفاعل مع reagent السكر، فمثلا لو
+
+451
+00:35:58,640 --> 00:36:03,040
+تفاعل، ممكن يعطيني مادة Z، مش Y، لأنها move، طب
+
+452
+00:36:03,040 --> 00:36:10,460
+أنا ما بقدر أقارن المادة Y في العينة مجهولة التركيز
+
+453
+00:36:10,460 --> 00:36:15,920
+اللي لونها pink، تمام، مع المادة Z اللي نتجت من تيوب
+
+454
+00:36:15,920 --> 00:36:20,100
+ال standard اللي لونها move، غلط، ما بنفع، عشان هيك
+
+455
+00:36:20,100 --> 00:36:23,260
+لازم يكونوا the same، نفس المادة، نفس التركيب، ونفس
+
+456
+00:36:23,260 --> 00:36:26,740
+ال reaction يحدث عشان يعطيني نفس ال product ونفس
+
+457
+00:36:26,740 --> 00:36:30,860
+اللون بغض النظر عن شدته، تمام، لإنه ممكن التركيز
+
+458
+00:36:30,860 --> 00:36:33,960
+العينة يكون مختلف عن تركيز ال standard، لازم
+
+459
+00:36:33,960 --> 00:36:38,530
+يعطيني نفس المادة، نفس اللون، نفس نوع اللون يعني
+
+460
+00:36:38,530 --> 00:36:43,430
+pink, mauve, blue, green, yellow، هذه الألوان يعني
+
+461
+00:36:43,430 --> 00:36:47,550
+بس أنا بحكي على نوع اللون، يعني اسم اللون، تمام؟ و
+
+462
+00:36:47,550 --> 00:36:52,890
+لحتى أيضا يكون لهم نفس الـ small اللي هو ال
+
+463
+00:36:52,890 --> 00:36:57,450
+coefficient of absorptivity of substance عشان يتم
+
+464
+00:36:57,450 --> 00:37:01,110
+تطبيق قانون بير لامبرت صحيح، ويروح الـ a مع الـ a، ويروح الـ
+
+465
+00:37:01,110 --> 00:37:07,080
+b مع الـ b، ويتم تطبيق قانون بير لامبرت، طيب إيه
+
+466
+00:37:07,080 --> 00:37:11,880
+instrument requirements؟ الشروط الواجب توفرها في
+
+467
+00:37:11,880 --> 00:37:17,060
+الجهاز، طبعا لازم يكون at a satisfactory level، يعني
+
+468
+00:37:17,060 --> 00:37:21,380
+درجة مرضية من ال accuracy، الدقة، ال sensitivity،
+
+469
+00:37:21,380 --> 00:37:25,520
+الحساسية، and reproducibility، منتج يعني ما.. يعني
+
+470
+00:37:25,520 --> 00:37:28,860
+ما بده يقرأ لعينتين تلاتة و يطلع بده صيانة، بده إيه
+
+471
+00:37:28,860 --> 00:37:33,740
+عدد calibration، بده أرجع شغله مرة تانية، بي
+
+472
+00:37:33,740 --> 00:37:39,620
+بيعلج مثلا أو إشي، هذه كل هالشغلات لازم يكون طبعا
+
+473
+00:37:39,850 --> 00:37:45,130
+بيشتغل بكفاءة عالية، ده cubits، الأنابيب اللي إحنا
+
+474
+00:37:45,130 --> 00:37:52,930
+بنستخدمهم لحتى يتم إدراجهم داخل الجهاز، وفيهم
+
+475
+00:37:52,930 --> 00:37:57,790
+المحلول الملون ويتم قياس عبرهم، هادول لازم يكونوا
+
+476
+00:37:57,790 --> 00:38:02,840
+أول شيء clean، وأيضا free from scratches، إيش يعني
+
+477
+00:38:02,840 --> 00:38:08,580
+free from scratches؟ يعني خالي من الشقوق، الكسور،
+
+478
+00:38:08,580 --> 00:38:17,960
+الخدوش، الشطوب، لازم تكون ملساء وتكون سليمة، ال
+
+479
+00:38:17,960 --> 00:38:21,240
+tube هاي ما فيها أي خدوش أو شقوق أو كسور، لإن
+
+480
+00:38:21,240 --> 00:38:25,480
+هذا بيأثر على مسار الضوء من خلالها، وجزء من الضوء
+
+481
+00:38:25,480 --> 00:38:32,180
+راح يتبدد بسبب أنها مشققة أو عليها كسور أو خدوش
+
+482
+00:38:32,180 --> 00:38:38,600
+أو لأنها مش نظيفة من برا، فبالتالي هذا بيأثر على
+
+483
+00:38:38,770 --> 00:38:42,850
+كمية الضوء اللي بيقرأها الجهاز أو يسجلها الجهاز
+
+484
+00:38:42,850 --> 00:38:49,090
+طيب لو أنا جيت  أصور التصور منطقي من ايش مكون
+
+485
+00:38:49,090 --> 00:38:53,590
+الجهاز؟ لابد أن يكون مكون من light source، مصدر
+
+486
+00:38:53,590 --> 00:38:58,130
+للضوء، بيعطيني أطوال موجية معينة، ولكن هناك طول موجي
+
+487
+00:38:58,130 --> 00:39:02,150
+واحد اللي يجب أن يخترق إلى الـ يعني أو يصل إلى
+
+488
+00:39:02,150 --> 00:39:04,830
+العينة أو المحلول، الـ tube اللي فيها
+
+489
+00:39:04,830 --> 00:39:08,150
+العينة أو الـ tube اللي فيها المحلول، طبعا فلابد من
+
+490
+00:39:08,150 --> 00:39:11,310
+أن يكون ما بين ال light source وال sample holder
+
+491
+00:39:11,310 --> 00:39:16,890
+يكون في عندي wavelength selector، تمام؟ وهو عازل
+
+492
+00:39:16,890 --> 00:39:22,230
+أو بيعزل طول موجي واحد ليعبر من خلال الـ tube
+
+493
+00:39:22,230 --> 00:39:27,110
+ليش؟ لأن هذا الطول الموجي لابد أن يكون عليه أقصى
+
+494
+00:39:27,110 --> 00:39:32,530
+كمية امتصاص من المحلول المراد قياسه، طبعا جزء من
+
+495
+00:39:32,530 --> 00:39:36,570
+الضوء سيتم امتصاصه وجزء سينفذ، راح يروح إلى ال
+
+496
+00:39:36,570 --> 00:39:40,130
+detector، هذا ال detector راح يحول الطاقة الضوئية
+
+497
+00:39:40,130 --> 00:39:45,430
+اللي وصلته إلى طاقة كهربائية، وراح يطلع لي، لابد أن
+
+498
+00:39:45,430 --> 00:39:49,070
+يكون في عندي شاشة أو مكان أقرأ عليه النتيجة، فراح
+
+499
+00:39:49,070 --> 00:39:52,630
+يكون في عندي read out، مكان تطلع عليه النتيجة تبعتي
+
+500
+00:39:52,630 --> 00:39:58,720
+والنتيجة يا بنات بتكون عبارة عن شدة التيار، معبر عن
+
+501
+00:39:58,720 --> 00:40:03,200
+كمية الضوء، إما ال transmittance أو ال absorbance
+
+502
+00:40:03,200 --> 00:40:07,320
+طبعا؟ طب على أي أساس هو بيختار، ليه يعطيني ال
+
+503
+00:40:07,320 --> 00:40:10,360
+transmittance أو ال absorbance؟ أنا بختار لما
+
+504
+00:40:10,360 --> 00:40:16,440
+برمج الجهاز، أو لما بدي أجهز الجهاز لقراءة فحص معين
+
+505
+00:40:16,440 --> 00:40:22,040
+أنا بختار هذا الطول الموجي اللي بده الـ طول الموجي المحدد
+
+506
+00:40:22,040 --> 00:40:25,660
+اللي بده يصطدم بالعينة، وأنا بختار الجهاز يعطيني
+
+507
+00:40:25,660 --> 00:40:28,540
+قراءة Transmittance، إذا Transmittance، قعدوا الـ bus
+
+508
+00:40:28,540 --> 00:40:31,920
+بده يحسب الضوء اللي بيواصل لل detector، يحولوا لطيار
+
+509
+00:40:31,920 --> 00:40:35,090
+كهربائي ويطلعوا على readout، إذا بدي إياه يقرأ لي
+
+510
+00:40:35,090 --> 00:40:37,790
+absorbance، معناها هو بدي إياه يحسب الـ
+
+511
+00:40:37,790 --> 00:40:41,910
+transmittance ويطرحه من الضوء الأساسي ويحسب لي
+
+512
+00:40:41,910 --> 00:40:46,450
+كمية ال absorbance، وهذا ال absorbance هو اللي
+
+513
+00:40:46,450 --> 00:40:52,310
+راح يتحول لطيار كهربائي ويعطيني قيمته على ال
+
+514
+00:40:52,310 --> 00:40:55,950
+readout، إذا أنا بحدد شغلتين، أنا بحدد ال
+
+515
+00:40:55,950 --> 00:40:59,070
+wavelength، الطول الموجي المعين اللي بيعزله ال
+
+516
+00:40:59,070 --> 00:41:02,530
+selector، وأنا بحدد القراءة تظهر على هيئة
+
+517
+00:41:02,530 --> 00:41:06,820
+transmittance أو absorbance، تمام؟ طيب كيف بـ بحدد
+
+518
+00:41:06,820 --> 00:41:11,540
+هذه الشركات والفحوصات اللي بتجيني من الشركة
+
+519
+00:41:11,540 --> 00:41:15,640
+بيحكي لي والله فحص السكر يفضل أنك أنت تقرأ إيه على
+
+520
+00:41:15,640 --> 00:41:20,400
+طول موجي 500، فحص الهيموجلوبين يفضل أنك أنت تقرأ
+
+521
+00:41:20,400 --> 00:41:24,320
+إيه على طول موجي 540، حسب لون الـ
+
+522
+00:41:24,320 --> 00:41:29,240
+المحلول الناتج بعد التفاعل، تمام؟ أو الـ final product
+
+523
+00:41:29,240 --> 00:41:34,340
+اللي ناتج بيحدده الشركات، الطول الموجي المناسب
+
+524
+00:41:34,340 --> 00:41:38,300
+لقراءة النتيجة عليه اللي بيكون عنده أقصى كمية
+
+525
+00:41:38,300 --> 00:41:44,670
+امتصاص وأقل كمية transmittance، تمام؟ إذن لازم يكون
+
+526
+00:41:44,670 --> 00:41:48,210
+في عندي stable source of radiation energy، مصدر
+
+527
+00:41:48,210 --> 00:41:52,630
+ثابت للطاقة، لازم يكون في عندي أداة that isolates a
+
+528
+00:41:52,630 --> 00:41:55,950
+restricted region of the spectrum، لازم يكون عندي
+
+529
+00:41:55,950 --> 00:42:04,310
+أداة تعزل طول موجي محدد من الطيف أو من الضوء، transparent container، هيكون في
+
+530
+00:42:04,310 --> 00:42:08,210
+عندي وعاء شفاف، هذا يوضع فيه المحلول الملون أو
+
+531
+00:42:08,210 --> 00:42:15,020
+ناتج التفاعل أو اللي هي نتائج الفحص، على اللي يتم
+
+532
+00:42:15,020 --> 00:42:18,740
+قراءته، لازم يكون في عندي detector، وهذا ال
+
+533
+00:42:18,740 --> 00:42:21,980
+detector وظيفته أنه يحول اللي هو ال radiation
+
+534
+00:42:21,980 --> 00:42:25,420
+energy أو الطاقة الضوئية إلى electrical energy، إلى
+
+535
+00:42:25,420 --> 00:42:29,420
+طاقة كهربائية، وهذه كمية التيار الكهربائي أو شدة
+
+536
+00:42:29,420 --> 00:42:34,860
+التيار الكهربائي اللي بينتج من ال detector بيظهر
+
+537
+00:42:34,860 --> 00:42:40,770
+على ال readout، على شاشة أو مكان مخصص لقراءة
+
+538
+00:42:40,770 --> 00:42:47,650
+النتائج، تمام؟ إذا هذا هو تمثيل لمكونات الجهاز من
+
+539
+00:42:47,650 --> 00:42:53,510
+الداخل سواء كان photometry أو visible يعني UV
+
+540
+00:42:53,510 --> 00:42:58,150
+light photometry أو visible photometry، طيب ال
+
+541
+00:43:02,650 --> 00:43:08,670
+slides القادمة رح تتحدث عن مكونات أو تفاصيل عن هذه
+
+542
+00:43:08,670 --> 00:43:12,550
+المكونات، تفاصيل مكونات الجهاز، نبدأ بال light
+
+543
+00:43:12,550 --> 00:43:17,990
+source، هلأ في عندي أجهزة بيعطيني ال light source
+
+544
+00:43:17,990 --> 00:43:21,890
+مصدر الضوء بيعطيني أطوال موجية تقع في منطقة ال
+
+545
+00:43:21,890 --> 00:43:26,810
+ultraviolet، احنا هنا ذكرنا إنه في عندي مناطق أو
+
+546
+00:43:26,810 --> 00:43:31,490
+regions من الطاقة الضوئية وكل طاقة ضوئية إلها
+
+547
+00:43:31,490 --> 00:43:36,370
+طول موجي معين، في عندي أجهزة بتعطيني أطوال موجية
+
+548
+00:43:36,370 --> 00:43:40,190
+تقع في منطقة ال ultraviolet، طب يعني ال ultraviolet
+
+549
+00:43:40,190 --> 00:43:45,880
+هدي إيش هيكون range الأطوال الموجية تبعتها؟ من 160
+
+550
+00:43:45,880 --> 00:43:51,180
+إلى 375، هذه اللمبة اللي بتعطيني أطوال موجية تقع في
+
+551
+00:43:51,180 --> 00:43:54,560
+منطقة ال ultraviolet اللي هو الأشعة فوق البنفسجية
+
+552
+00:43:54,560 --> 00:43:59,000
+واللي بتعطيني أطوال موجية في range، طبعا أطوال
+
+553
+00:43:59,000 --> 00:44:06,140
+موجية في range ما بين 160 إلى 375 بتكون إما لمبة
+
+554
+00:44:06,140 --> 00:44:10,220
+Deuterium أو Hydrogen، مصنوعة من ال Deuterium أو ال
+
+555
+00:44:10,220 --> 00:44:15,140
+Hydrogen، تمام؟ إذا بتكون في عندي لمبة Deuterium أو
+
+556
+00:44:15,140 --> 00:44:19,760
+Hydrogen بتعطيني أطوال موجية معينة يعني في range
+
+557
+00:44:19,760 --> 00:44:25,340
+ما بين 160 إلى 375، وفي النهاية ال selector بيعزل لي
+
+558
+00:44:25,340 --> 00:44:29,480
+طول موجي واحد فقط ليصطدم بالعينة وبيكمل الشغل
+
+559
+00:44:29,480 --> 00:44:34,800
+تمام؟ في ال visible region، إذا كان الجهاز بيعطيني
+
+560
+00:44:34,800 --> 00:44:40,250
+أو بيقرأ فقط في منطقة الضوء المرئي، تمام؟ معناها
+
+561
+00:44:40,250 --> 00:44:44,210
+هيعطيني ال light source أطوال موجية ما بين 350
+
+562
+00:44:44,210 --> 00:44:49,090
+إلى 2500 نانومتر، واللمبة اللي هتكون موجودة في الجهاز
+
+563
+00:44:49,090 --> 00:44:52,310
+هي تنجستن، إذا في فحوصات بتنقرأ على منطقة ال UV
+
+564
+00:44:52,310 --> 00:44:56,010
+light وفي فحوصات بتنقرأ على ال visible، ال UV
+
+565
+00:44:56,010 --> 00:45:01,190
+light بيكون الجهاز اللي بيقرأ الفحوصات هاي ال
+
+566
+00:45:01,190 --> 00:45:04,270
+light source تبعه إما Deuterium lamp أو hydrogen
+
+567
+00:45:04,270 --> 00:45:07,550
+lamp، والأطوال الموجية اللي بتوفرها هاي ال lamp من
+
+568
+00:45:07,550 --> 00:45:12,010
+160 إلى 375، أما الفحوصات اللي بتقرأ على ال visible
+
+569
+00:45:12,010 --> 00:45:18,090
+region، فـ أو في ال visible photometer بيكون ال
+
+570
+00:45:18,090 --> 00:45:21,830
+الجهاز اللمبة تبعته عبارة عن tungsten وبتعطيني
+
+571
+00:45:21,830 --> 00:45:26,710
+أطوال موجية ما بين 350 إلى 2500 نانومتر، احنا عندنا
+
+572
+00:45:26,710 --> 00:45:31,550
+جهاز مكتوب عليه UV-visible spectrophotometer، يعني بيعطيني أطوال موجية في
+
+573
+00:45:31,550 --> 00:45:35,200
+منطقة ال ultraviolet وفي ال visible، الفحوصات اللي
+
+574
+00:45:35,200 --> 00:45:38,320
+بتنقرأ على ال UV light ممكن أقرأها عليه، والفحوصات
+
+575
+00:45:38,320 --> 00:45:41,960
+اللي بتنقرأ على ال visible أيضا بتنقرأ عليه، إذا هو
+
+576
+00:45:41,960 --> 00:45:45,280
+هيزود لي أطوال موجية ما بين 160 إلى 2500 نانومتر
+
+577
+00:45:45,280 --> 00:45:50,640
+وهيكون عنده لمبتين، أو two lamps، ال Deuterium
+
+578
+00:45:50,640 --> 00:45:55,310
+و Hydrogen Lamp، Deuterium أو Hydrogen، هذه لما
+
+579
+00:45:55,310 --> 00:45:59,950
+بدي لما بدي أدخل طول موجي في منطقة ال UV
+
+580
+00:45:59,950 --> 00:46:03,590
+مثلا 200 أو 260، معناها هو هيحول هيشغل ال
+
+581
+00:46:03,590 --> 00:46:08,550
+Deuterium أو ال Hydrogen Lamp، ويعطيني الطول الموجي
+
+582
+00:46:08,550 --> 00:46:12,150
+اللي بدي إياه، طبعا؟ لو أنا مثلا بدي أقرأ على 400 أو
+
+583
+00:46:12,150 --> 00:46:16,030
+540، اللي هو هيموجلوبين، بنقرأ على 540 يعني على منطقة
+
+584
+00:46:16,030 --> 00:46:19,670
+ال visible، طبعا؟ معناها اللي هتشتغل ال tungsten
+
+585
+00:46:19,670 --> 00:46:23,340
+بيكون عنده two types of lamp وبيشغل واحدة منهم
+
+586
+00:46:23,340 --> 00:46:28,060
+حسب نوع الطول الموجي اللي أنا دخلته، احنا حكينا
+
+587
+00:46:28,060 --> 00:46:31,280
+الطول الموجي أنا بدخله وأنا اللي بحدده، وبيكون
+
+588
+00:46:31,280 --> 00:46:34,900
+الشركة اللي أعطتني إياه، تمام، تبع الفحص، طيب ال
+
+589
+00:46:34,900 --> 00:46:40,800
+wavelength selector هذا اللي بيعزل طول موجي معين
+
+590
+00:46:40,800 --> 00:46:44,280
+اللي هو يتم إدخاله للجهاز، هذا بيكون إما filter أو
+
+591
+00:46:44,280 --> 00:46:49,660
+monochromator، طبعا إذا كان filter فهو بيكون مصنوع
+
+592
+00:46:49,660 --> 00:46:55,520
+من طبقة transparent dielectric layer، طبقة شفافة
+
+593
+00:46:55,520 --> 00:46:59,960
+مقطبة، الها أقطاب، ومصنوعة من ال calcium fluoride
+
+594
+00:46:59,960 --> 00:47:04,020
+وسُمك هاي الطبقة هو اللي بيتحكم بالطول الموجي اللي
+
+595
+00:47:04,020 --> 00:47:08,000
+بيمر من خلالها، أو يعني إذا ما كان من ال transparent
+
+596
+00:47:08,000 --> 00:47:13,930
+dielectric layer of calcium chloride، معناها بدي
+
+597
+00:47:13,930 --> 00:47:18,430
+يكون مصنوع من colored glass، تمام؟ إذا calcium
+
+598
+00:47:18,430 --> 00:47:22,630
+fluoride layer أو colored glass، هذا ال filter، تمام؟
+
+599
+00:47:28,820 --> 00:47:31,740
+أو بيكون ال selector مش filter، بيكون
+
+600
+00:47:31,740 --> 00:47:34,380
+monochromator، إيش يعني monochromator؟ ال
+
+601
+00:47:34,380 --> 00:47:38,840
+monochromator هذا بيكون إما grating أو prism، أنا
+
+602
+00:47:38,840 --> 00:47:43,560
+أقدر إنه المصطلحات هذه أول مرة تمر عليكم، ولكن
+
+603
+00:47:43,560 --> 00:47:46,940
+إذا أنتم يعني بس يعني انتبهتوا للكلام اللي بحكي
+
+604
+00:47:46,940 --> 00:47:51,340
+هو فقط اللي مطلوب منكم، وهيكون واضح بالنسبة لكم
+
+605
+00:47:51,340 --> 00:47:55,450
+إذا ال selector هذا ممكن يكون filter، ال
+
+606
+00:47:55,450 --> 00:47:58,910
+filter هذا ممكن يكون طبقة transparent dielectric
+
+607
+00:47:58,910 --> 00:48:02,570
+layer مصنوعة من الكالسيوم فلوريد، وسُمك هذه الطبقة
+
+608
+00:48:02,570 --> 00:48:06,190
+هو اللي بيتحكم بالطول الموجي، كم طول موجي بيمر من
+
+609
+00:48:06,190 --> 00:48:13,220
+خلالها، يعني طول الموجي يعني قيمته، تمام، أو أو إنه
+
+610
+00:48:13,220 --> 00:48:17,420
+يكون هذا ال filter مصنوع من الزجاج الملون، ومن
+
+611
+00:48:17,420 --> 00:48:22,060
+شوية، ضربنا مثال، حكينا الزجاج لو كان ملون وحطيته
+
+612
+00:48:22,060 --> 00:48:25,700
+أنا أمام لمبة، هيحجز الضوء، هيحجب الضوء المار،
+
+613
+00:48:25,700 --> 00:48:30,890
+تمام؟ يعني فكرة عمل filter، طيب إذا كان ال wavelength selector عبارة عن monochromator، فبيكون
+
+614
+00:48:30,890 --> 00:48:33,510
+615
+00:48:33,510 --> 00:48:37,250
+إما a grating أو a prism، تمام؟ إيش يعني grating؟
+
+616
+00:48:37,250 --> 00:48:41,270
+ال grating هو عبارة عن قطعة زجاجية مشرشرة، تمام؟
+
+617
+00:48:41,2
+
+667
+00:52:24,210 --> 00:52:27,650
+فيهم إما photocell أو photomultiplier tube
+
+668
+00:52:27,650 --> 00:52:30,790
+ويقيسوا كمية الضوء التي نفذت عبر المحلول الملون
+
+669
+00:52:30,790 --> 00:52:34,640
+ليُعطوا كمية إما transmittance أو absorbance
+
+670
+00:52:34,640 --> 00:52:39,680
+تمام، هذا كله كلام لا خلاف عليه، ولكن يختلفون في نوع
+
+671
+00:52:39,680 --> 00:52:43,080
+الـ wavelength selector الموجود فيهم. فالـ
+
+672
+00:52:43,080 --> 00:52:46,540
+photometer فيه filter، يعني إما calcium fluoride أو
+
+673
+00:52:46,540 --> 00:52:49,660
+color glass، والـ spectrophotometer فيه
+
+674
+00:52:49,660 --> 00:52:54,120
+monochromator، يعني إما grating أو prism. تمام؟
+
+675
+00:52:54,120 --> 00:53:00,000
+طيب، الـ sample container، هذه الحاوية تحتوي على المحلول
+
+676
+00:53:00,000 --> 00:53:04,740
+الملون، الكيوفِت. مرت معنا الكيوفِت كثيراً وذكرناها
+
+677
+00:53:04,740 --> 00:53:08,620
+وشرحنا ماذا تعني كلمة كيوفِت، وفي المعمل أيضاً راح
+
+678
+00:53:08,620 --> 00:53:12,480
+أُريكُم إياها وأُخبركُم تعليمات كيف نحن نستخدمها.
+
+679
+00:53:12,480 --> 00:53:17,290
+كيف نمسكها، كيف نشتغل فيها. فبالتالي، الـ high liquid هي
+
+680
+00:53:17,290 --> 00:53:21,530
+التي that hold the sample، بتحتوي... هي عبارة عن الـ
+
+681
+00:53:21,530 --> 00:53:26,190
+sample container، تحتوي الـ sample المراد قياسها أو
+
+682
+00:53:26,190 --> 00:53:30,730
+المحلول المراد قياسه في الجهاز. بتكون مصنوعة إما من
+
+683
+00:53:30,730 --> 00:53:35,430
+glass أو من quartz أو من silica. الـ silica تستخدم
+
+684
+00:53:35,430 --> 00:53:38,510
+في القراءات على الـ visible light، والـ glass
+
+685
+00:53:38,510 --> 00:53:43,710
+تُستخدم للقراءات على الـ UV light. طبعاً، نحن
+
+686
+00:53:43,710 --> 00:53:49,490
+كل شغلنا، معظم الشغل هذه الفترة، سيكون على الـ
+
+687
+00:53:49,490 --> 00:53:52,270
+visible، فستستخدمون الـ silica أو الـ plastic.
+
+688
+00:53:55,090 --> 00:53:59,450
+أما في... إن شاء الله في معامل متقدمة ستأخذون
+
+689
+00:53:59,450 --> 00:54:02,090
+فحوصات تعتمد على الـ UV light، وستلاحظون أن
+
+690
+00:54:02,090 --> 00:54:07,130
+الكيوفِت ستكون خاصة من الـ quartz أو
+
+691
+00:54:07,130 --> 00:54:11,150
+من الـ glass، لكي تشتغلوا عليها، تمام؟ على الـ UV
+
+692
+00:54:11,150 --> 00:54:14,320
+light. طيب، الـ detector، نحن حكينا أن الـ detector إما
+
+693
+00:54:14,320 --> 00:54:17,700
+سيكون photocell أو photomultiplier tube. الآن،
+
+694
+00:54:17,700 --> 00:54:20,380
+photo tube أو photocell، نفس المعنى، أو photo
+
+695
+00:54:20,380 --> 00:54:23,620
+multiplier tube. الـ photo  الذي يجب أن تعرفوا، أو الذي
+
+696
+00:54:23,620 --> 00:54:27,320
+مطلوب منكم، أن الـ photocell تعمل بطريقة الـ photo
+
+697
+00:54:27,320 --> 00:54:31,100
+conductivity. أما الـ photomultiplier، مبدأ عملها
+
+698
+00:54:31,100 --> 00:54:34,500
+باستخدام أقطاب السيليكون، silicon diode electrode.
+
+699
+00:54:34,500 --> 00:54:38,400
+الآن، كيف بالضبط، وتفاصيل أكثر حول مصطلح الـ photo
+
+700
+00:54:38,400 --> 00:54:42,500
+conductivity، التوصيلية الضوئية، أو تفاصيل حول الـ
+
+701
+00:54:42,500 --> 00:54:45,800
+silicon diode electrode، لن ندخل فيها. كل ما هو
+
+702
+00:54:45,800 --> 00:54:50,110
+مطلوب منكم، أن الـ photo tubes أو الـ photocells
+
+703
+00:54:50,110 --> 00:54:53,970
+تعمل بمبدأ الـ photo conductivity، والـ photo
+
+704
+00:54:53,970 --> 00:54:57,630
+multiplier tube تعمل بمبدأ الـ silicon diode
+
+705
+00:54:57,630 --> 00:55:02,370
+electrode. تمام؟ وهذا الـ detector، حكينا أنه يستقبل
+
+706
+00:55:02,370 --> 00:55:07,690
+الطاقة الضوئية من الضوء النافذ، ويحولها إلى طاقة
+
+707
+00:55:07,690 --> 00:55:11,510
+كهربائية، إلى electrical energy، عبر جهاز
+
+708
+00:55:11,510 --> 00:55:16,860
+galvanometer موجود داخل الـ detector. تمام؟ سواء كان
+
+709
+00:55:16,860 --> 00:55:19,940
+photocell أو photomultiplier tube. فلنرى فقط كيف
+
+710
+00:55:19,940 --> 00:55:24,240
+يتم تحويل الطاقة الضوئية إلى طاقة كهربائية.
+
+711
+00:55:24,240 --> 00:55:28,960
+الآن، ما يحدث هو أن الضوء الذي نفذ عبر المحلول، لـ
+
+712
+00:55:28,960 --> 00:55:32,580
+transmittance، سيصل إلى الـ detector. أولاً، طبعاً الـ
+
+713
+00:55:32,580 --> 00:55:35,960
+detector فيه cathode وفيه anode، قطب موجب وقطب
+
+714
+00:55:35,960 --> 00:55:41,940
+سالب. هذا الضوء سيصطدم بالقطب السالب، وهو فوتونات،
+
+715
+00:55:41,940 --> 00:55:45,620
+تمام؟ هذه الفوتونات ستصطدم بالقطب السالب الذي
+
+716
+00:55:45,620 --> 00:55:49,560
+هو الـ cathode، فسيتحرر من هذا الـ cathode
+
+717
+00:55:49,560 --> 00:55:55,020
+إلكترونات، تمام؟ وهذه الإلكترونات ستتجه باتجاه
+
+718
+00:55:55,020 --> 00:55:59,340
+الأنود، القطب الموجب، تمام؟ هذا هو اتجاهها
+
+719
+00:55:59,340 --> 00:56:05,800
+للإلكترونات، باتجاه القطب الموجب. تمام؟ طيب، سريان
+
+720
+00:56:05,800 --> 00:56:10,500
+الإلكترونات أو الشحنات السالبة من القطب السالب إلى القطب
+
+721
+00:56:10,500 --> 00:56:14,720
+الموجب، هذا يعني أنه يوجد عندي دارة كهربائية يمر
+
+722
+00:56:14,720 --> 00:56:19,300
+فيها تيار. تمام؟ جهاز الـ galvanometer سيقيس
+
+723
+00:56:19,300 --> 00:56:27,370
+كمية شدة التيار الذي نشأ. نشأ نتيجة ماذا؟ نتيجة
+
+724
+00:56:27,370 --> 00:56:33,570
+انبعاث ضوء عبر المحلول الملون، نفذ من خلاله
+
+725
+00:56:33,570 --> 00:56:39,250
+هذا الضوء، ضرب في الـ cathode، وتحررت الإلكترونات.
+
+726
+00:56:39,250 --> 00:56:42,510
+هذه الإلكترونات انتقلت من القطب السالب إلى القطب
+
+727
+00:56:42,510 --> 00:56:47,170
+الموجب. إذن، أنتِ لديكِ ضوء photon، وكمية transmitted
+
+728
+00:56:47,170 --> 00:56:51,250
+light، راحت إلى الـ cathode، حررت إلكترونات.
+
+729
+00:56:51,250 --> 00:56:56,490
+هذه الإلكترونات تحركت إلى الـ anode، بتكون عندي تيار.
+
+730
+00:56:56,490 --> 00:57:00,990
+شدة التيار تُقاس بالـ galvanometer، و ستظهر على الـ
+
+731
+00:57:00,990 --> 00:57:04,930
+readout إما كـ transmittance، كقيمة transmittance، أو
+
+732
+00:57:04,930 --> 00:57:09,610
+ستظهر كقيمة absorbance. من الذي يحدد أن الجهاز
+
+733
+00:57:09,610 --> 00:57:12,910
+يعمل حساباته، ويقيس للـ absorbance، أو يقيس للـ
+
+734
+00:57:12,910 --> 00:57:17,360
+transmittance directly؟ أنا التي أ... أ... أدخل الـ
+
+735
+00:57:17,360 --> 00:57:20,880
+mode of read، يعني أنتِ ماذا تقرئين لي يا جهاز؟ تقرئين لي
+
+736
+00:57:20,880 --> 00:57:24,680
+absorbance أم تقرئين لي transmittance؟ وكل تعاملاتنا
+
+737
+00:57:24,680 --> 00:57:28,800
+أو معظم تعاملاتنا تكون، يا بنات، بالـ absorbance، و
+
+738
+00:57:28,800 --> 00:57:34,970
+ليس بالـ transmittance. تمام؟ طيب، بالنسبة لجهاز آخر
+
+739
+00:57:34,970 --> 00:57:38,930
+تمام، اسمه flame photometry أو flame emission
+
+740
+00:57:38,930 --> 00:57:43,450
+spectroscopy. هو جهاز يعمل بنفس مبدأ عمل الـ
+
+741
+00:57:43,450 --> 00:57:47,350
+spectrophotometer. Flame photometry is a spectral
+
+742
+00:57:47,350 --> 00:57:51,130
+method in which excitation is caused by spraying a
+
+743
+00:57:51,130 --> 00:57:54,970
+solution of the sample in a hot flame. طبعاً، هو
+
+744
+00:57:54,970 --> 00:57:59,640
+عبارة عن... الجهاز يعمل أيضاً بطريقة spectral method
+
+745
+00:57:59,640 --> 00:58:04,100
+بطريقة قياس نوع الضوء أو كمية الضوء من خلال أن
+
+746
+00:58:04,100 --> 00:58:09,320
+أنا أقوم بـ spraying للعينة على لهب. إذاً، مصدر الضوء
+
+747
+00:58:09,320 --> 00:58:14,300
+هنا light source، لهب وليس لمبة. تمام؟ هذا
+
+748
+00:58:14,300 --> 00:58:18,240
+الاختلاف. Characteristic radiation is emitted in
+
+749
+00:58:18,240 --> 00:58:21,360
+flame by individual element and the emission
+
+750
+00:58:21,360 --> 00:58:24,800
+intensity is proportional to the concentration of
+
+751
+00:58:24,800 --> 00:58:28,660
+the element introduced into the flame. شوفوا معايا
+
+752
+00:58:29,370 --> 00:58:34,970
+الآن، كمية الضوء التي تنبعث من هذا
+
+753
+00:58:34,970 --> 00:58:42,470
+اللهب تتناسب تناسباً طردياً مع تركيز المواد التي
+
+754
+00:58:42,470 --> 00:58:47,310
+موجودة في العينة. هذا الجهاز يُستخدم لقياس الـ
+
+755
+00:58:47,310 --> 00:58:50,850
+electrolytes في العينة، في عينات الدم أو سواء
+
+756
+00:58:50,850 --> 00:58:55,560
+الجسم. تمام؟ ماذا تعني electrolytes؟ تعني الأيونات
+
+757
+00:58:55,560 --> 00:58:58,540
+Sodium, Potassium, Calcium, Magnesium, Fluoride,
+
+758
+00:58:58,640 --> 00:59:03,560
+Chloride, Sodium. هذه كلها عبارة عن أيونات يتم
+
+759
+00:59:03,560 --> 00:59:08,760
+قياسها بطريقة الفليم فوتومتري أو الفليم Emission
+
+760
+00:59:08,760 --> 00:59:12,980
+Spectroscopy. ماذا يحدث في هذا الجهاز؟ أولاً، أنا
+
+761
+00:59:12,980 --> 00:59:17,400
+ليس لدي كيوفِت، يعني أنا لا أدخل الأنبوبة أو يعني
+
+762
+00:59:17,400 --> 00:59:21,840
+المحلول في أنبوبة كيوفِت، وأدخله للجهاز. الجهاز يتلقى
+
+763
+00:59:21,840 --> 00:59:25,680
+العينة بطريقة الـ suction. إذاً، فيه زي suction، زي
+
+764
+00:59:25,680 --> 00:59:34,140
+... أداة أو جزء يمتص العينة، امتصاص. تمام؟ وهذه
+
+765
+00:59:34,140 --> 00:59:38,280
+العينة التي يتم امتصاصها تدخل إلى حجرة داخل
+
+766
+00:59:38,280 --> 00:59:44,820
+الجهاز، مكان صغير مخصص للعينة. هذه الحجرة درجة
+
+767
+00:59:44,820 --> 00:59:49,860
+حرارتها عالية جداً، لأن يوجد في أعلاها لهب.
+
+768
+00:59:49,860 --> 00:59:55,740
+لهب. طبعاً، نتيجة درجة الحرارة العالية، هذه العينة
+
+769
+00:59:55,740 --> 01:00:01,440
+السائلة، سواء دم أو سوائل الجسم، ستتحول من...
+
+770
+01:00:01,440 --> 01:00:06,920
+من حالة سائلة إلى حالة ما بين السائلة والغازية،
+
+771
+01:00:06,920 --> 01:00:12,460
+وهي الـ spray، ستصبح رذاذاً. طبعاً، هذا الرذاذ
+
+772
+01:00:12,460 --> 01:00:19,060
+يتصاعد، ويعني يتصاعد باتجاه الـ flame، باتجاه اللهب.
+
+773
+01:00:19,470 --> 01:00:27,630
+إذاً، يتصاعد باتجاه اللهب. إذن، تمام، سيُغيّر لي خواص
+
+774
+01:00:27,630 --> 01:00:33,870
+اللهب، سيُغيّر لون اللهب. تمام؟ إذاً، العينة التي يتم امتصاصها
+
+775
+01:00:33,870 --> 01:00:38,410
+تذهب إلى حجرة، هذه الحجرة اسمها nebulizer. هذه
+
+776
+01:00:38,410 --> 01:00:42,870
+الحجرة، طبعاً، درجة حرارتها عالية، لأن يوجد عندي على
+
+777
+01:00:42,870 --> 01:00:50,080
+أعلاها، أو على سقفها، لهب. فهي العينة التي دخلت إلى الـ
+
+778
+01:00:50,080 --> 01:00:55,940
+nebulizer، تتحول من حالة سائلة إلى حالة ما بين
+
+779
+01:00:55,940 --> 01:01:00,060
+السائلة والغازية، وهي حالة الـ spray، تصبح رذاذاً.
+
+780
+01:01:00,060 --> 01:01:07,220
+الرذاذ هذا يتصاعد، وطبعاً يؤثر على خواص اللهب. تمام؟
+
+781
+01:01:07,220 --> 01:01:11,740
+هذا يؤثر على خواص اللهب. مثلاً، لو كنت أعرف أن الـ sodium
+
+782
+01:01:11,740 --> 01:01:16,660
+يُعطيني اللهب لونه أحمر، نفترض مثلاً أن اشتعال الـ
+
+783
+01:01:16,660 --> 01:01:19,840
+sodium سيُعطيني لون أحمر، سيُغيّر لون اللهب للون
+
+784
+01:01:19,840 --> 01:01:25,240
+الأحمر. إذاً، يوجد هنا أشعة تجمع اللون الأحمر.
+
+785
+01:01:25,240 --> 01:01:29,820
+كمية اللون الأحمر الموجودة تتجمع، وتمرّ من
+
+786
+01:01:29,820 --> 01:01:33,220
+خلال filter. الـ filter هذا يُمرّر الضوء بشكل عام
+
+787
+01:01:33,220 --> 01:01:36,600
+الذي يأتي من اللهب. يتجمع ويذهب إلى الـ filter. الـ
+
+788
+01:01:36,600 --> 01:01:40,140
+filter سيُمرّر فقط الضوء الأحمر، سيذهب إلى الـ
+
+789
+01:01:40,140 --> 01:01:43,980
+photo detector، ويتحول الضوء إلى طاقة كهربائية، و
+
+790
+01:01:43,980 --> 01:01:46,940
+تظهر على الـ readout. هنا، الـ readout الذي يظهر
+
+791
+01:01:46,940 --> 01:01:52,440
+يتناسب تناسباً طردياً مع ما هو emitted. تمام؟ ولكن
+
+792
+01:01:52,440 --> 01:01:57,540
+يتناسب تناسباً طردياً مع تركيز الـ sodium.
+
+793
+01:01:57,540 --> 01:02:01,580
+الذي يوجد، سيُعطيني لوناً أحمر شديداً. إذاً، كمية القراءة
+
+794
+01:02:01,580 --> 01:02:05,320
+التي نتجت، نتيجة التناسب الطردي، ستكون كمية الضوء التي
+
+795
+01:02:05,320 --> 01:02:09,940
+وصلت إلى الـ detector ستكون كبيرة، فبالتالي يتناسب
+
+796
+01:02:09,940 --> 01:02:14,340
+طردياً مع الـ sodium. نفترض أن اشتعال البوتاسيوم يُنتج
+
+797
+01:02:14,340 --> 01:02:19,400
+عنه لون أزرق، إذاً، سيُغيّر خواص اللهب، ويصبح فيه لون
+
+798
+01:02:19,400 --> 01:02:25,600
+أزرق. فالعدسة الجامعة، هذه تجمع لي الضوء
+
+799
+01:02:25,600 --> 01:02:28,500
+كله ككل، وتُمرّره على الـ filter. الـ filter فقط
+
+800
+01:02:28,500 --> 01:02:31,420
+يُمرّر اللون الأزرق، يُمرّره... يُمرّره على الـ photo
+
+801
+01:02:31,420 --> 01:02:35,380
+detector، وهو الـ detector. فبالتالي، يحوّل الطاقة
+
+802
+01:02:35,380 --> 01:02:37,820
+الضوئية التي أتت من اللون الأزرق إلى طاقة
+
+803
+01:02:37,820 --> 01:02:40,900
+كهربائية، وتظهر على الـ read out. كمية الطاقة التي
+
+804
+01:02:40,900 --> 01:02:43,540
+ظهرت أو القراءة التي ظهرت، كلما كانت أعلى، يعني
+
+805
+01:02:43,540 --> 01:02:47,360
+كمية الضوء أعلى، يعني هنا أصبح لون أزرق كثير.
+
+806
+01:02:47,360 --> 01:02:51,680
+إذاً، يوجد مثلاً potassium اشتعاله كثير، هذه علاقة
+
+807
+01:02:51,680 --> 01:02:57,410
+حكينا، علاقة طردية، على عكس مثلاً أن الـ
+
+808
+01:02:57,410 --> 01:02:59,810
+spectrophotometer، حكينا في الـ spectrophotometer،
+
+809
+01:02:59,810 --> 01:03:01,910
+كمية الضوء التي وصلت إلى الـ detector هي الـ
+
+810
+01:03:01,910 --> 01:03:07,950
+transmittance، وتتناسب تناسباً عكسياً مع العينة، مع
+
+811
+01:03:07,950 --> 01:03:10,990
+تركيز العينة. إنما الـ absorbance الذي بدأنا به في
+
+812
+01:03:10,990 --> 01:03:14,430
+العينة، هو الذي يتناسب تناسباً طردياً مع العينة.
+
+813
+01:03:14,430 --> 01:03:18,070
+تمام؟ فحلو أن تفرقوا، وتربطوا الأمور ببعضها
+
+814
+01:03:18,070 --> 01:03:23,230
+البعض، لكي تعمقوا الفهم عندكم. هكذا نكون وصلنا
+
+815
+01:03:23,230 --> 01:03:26,860
+لنهاية محاضرتنا لهذا اليوم. يعطيكم ألف عافية. السلام عليكم.
+
diff --git a/PL9fwy3NUQKwY9jl8xw-bWkVZkWcGGyspd/pYa8qDaMM00_postprocess.srt b/PL9fwy3NUQKwY9jl8xw-bWkVZkWcGGyspd/pYa8qDaMM00_postprocess.srt
new file mode 100644
index 0000000000000000000000000000000000000000..be1eda29d11bfd0e6d0f81ce2a0815c02f68887c
--- /dev/null
+++ b/PL9fwy3NUQKwY9jl8xw-bWkVZkWcGGyspd/pYa8qDaMM00_postprocess.srt
@@ -0,0 +1,3260 @@
+1
+00:00:03,930 --> 00:00:07,410
+السلام عليكم ورحمة الله وبركاته طالبات العزيزات
+
+2
+00:00:07,410 --> 00:00:10,610
+اليوم ان شاء الله رح نشرح محاضرة بعنوان calorie
+
+3
+00:00:10,610 --> 00:00:15,090
+meter or photometer في البداية ماذا يعني calorie
+
+4
+00:00:15,090 --> 00:00:20,610
+meter? color معناها لون تمام؟ meter قياس إذا قياس
+
+5
+00:00:20,610 --> 00:00:26,040
+شدة اللون أو photometer photo معناها ضوءو meter
+
+6
+00:00:26,040 --> 00:00:31,540
+قياس أو قياس شدة الضوء قياس شدة اللون أو قياس شدة
+
+7
+00:00:31,540 --> 00:00:36,020
+الضوء هي طريقة تستخدم for concentration،
+
+8
+00:00:36,020 --> 00:00:39,060
+determination في ال medical laboratories في
+
+9
+00:00:39,060 --> 00:00:44,420
+المختبرات الطبية هذا مبدأ عمل أجهزة تستخدم في
+
+10
+00:00:44,420 --> 00:00:48,300
+معرفة التركيز أو قياس تركيز المحاليل و العينات
+
+11
+00:00:48,300 --> 00:00:53,290
+بمبدأ عمل ال calorie meter و ال photometerطيب ما
+
+12
+00:00:53,290 --> 00:00:59,010
+علاقة قياس شدة اللون أو الضوء بالتركيز راح نتعرف
+
+13
+00:00:59,010 --> 00:01:04,550
+خلال ال slides القادمة ولكن في البداية لابد من
+
+14
+00:01:04,550 --> 00:01:09,250
+تقديم بسيط انعرف فيه ماذا يعني photometry photo
+
+15
+00:01:09,250 --> 00:01:15,770
+حكينا معناها ضوء متري قياسإذن photometry معناها
+
+16
+00:01:15,770 --> 00:01:20,730
+the measurement of light قياس الضوء تمام؟ في عندي
+
+17
+00:01:20,730 --> 00:01:26,110
+أجهزة تسمى photometer أو spectrophotometer هي
+
+18
+00:01:26,110 --> 00:01:30,230
+عبارة عن أجهزة تعمل على مبدأ قياس الضوء the
+
+19
+00:01:30,230 --> 00:01:33,970
+measurement of lightو هذه الأجهزة they are
+
+20
+00:01:33,970 --> 00:01:40,750
+instruments used to detect the measurement أو to
+
+21
+00:01:40,750 --> 00:01:45,390
+detect the concentration و هذه الأجهزة يوجد فيها
+
+22
+00:01:45,390 --> 00:01:50,690
+أدوات أو أداة تسمى photocell أو photo multiplier
+
+23
+00:01:50,690 --> 00:01:54,940
+tubeهذه ال photocell أو ال photo multiplier tube
+
+24
+00:01:54,940 --> 00:01:59,880
+من خصائصها إنه لديها القدرة على إنها تقيس كمية
+
+25
+00:01:59,880 --> 00:02:06,240
+الضوء الواصلة إليها بعد مرورها بمحلول ملون، تمام؟
+
+26
+00:02:06,240 --> 00:02:11,490
+فإذا كان في عندي محلول ملونو .. او يعني reaction
+
+27
+00:02:11,490 --> 00:02:16,890
+حدث نتيجة مثلا ال combination الإضافة ما بين ال
+
+28
+00:02:16,890 --> 00:02:19,890
+sample و ال reagent حكينا ال reagent هو عبارة عن
+
+29
+00:02:19,890 --> 00:02:24,210
+إيش؟ هو عبارة عن محلول بيعمللي measurable change
+
+30
+00:02:25,160 --> 00:02:30,260
+تغير يمكن قياسه و هي على أغلب التغيرات ممكن تكون
+
+31
+00:02:30,260 --> 00:02:34,680
+تغير في اللون طبعا فإذا نتيجة reaction معين ما بين
+
+32
+00:02:34,680 --> 00:02:38,880
+ال sample و ال reagent نتج colored solution نتج
+
+33
+00:02:38,880 --> 00:02:46,320
+محلول ملون هذا المحلول إذا مر من خلاله ضوء فإن جزء
+
+34
+00:02:46,320 --> 00:02:51,920
+من هذا الضوء اللي مر أو اصطدم بهذا المحلولجزء من
+
+35
+00:02:51,920 --> 00:02:56,780
+هذا الضوء يتبدد ويتلاشى أو يتم انتصاصه من قبل
+
+36
+00:02:56,780 --> 00:03:03,080
+المحلول الملون والجزء الآخر ينفذ هذا الجزء النافذ
+
+37
+00:03:03,080 --> 00:03:08,200
+يصل إلى أداة في الجهاز اسمها photocell أو photo
+
+38
+00:03:08,200 --> 00:03:13,420
+multiplier tubeهى اللى بتقيسلى كمية الضوء اللى
+
+39
+00:03:13,420 --> 00:03:17,680
+واصلة إليها و بالنهاية بتعبرلى عن تركيز المحلول
+
+40
+00:03:17,680 --> 00:03:22,940
+الملون تمام؟ بتعبرلى عن تركيز المحلول الملون خلينا
+
+41
+00:03:22,940 --> 00:03:34,500
+بس لحتى نقرب الفكرة نحكي عن هذا الموضوع هى هنا
+
+42
+00:03:34,500 --> 00:03:41,270
+مثلا فى عندي lightضوء مصدر ضوء طبعا اصطدم بانبوب
+
+43
+00:03:41,270 --> 00:03:45,890
+فيها محلول ملون تمام اصطدم بانبوب فيها محلول ملون
+
+44
+00:03:45,890 --> 00:03:53,230
+جزء من هذا الضوء تم امتصاصه فقدانه او تلاشيه عبر
+
+45
+00:03:53,230 --> 00:03:57,270
+المحلول تمام او من خلال المحلول داخل المحلول تم
+
+46
+00:03:57,270 --> 00:04:03,970
+امتصاصهوالجزء الأخر نفذ تمام؟ هذا الجزء النافذ هو
+
+47
+00:04:03,970 --> 00:04:07,310
+الذي يصل إلى أداة ال photocell أو ال photo
+
+48
+00:04:07,310 --> 00:04:11,810
+multiplier tube في جهاز ال photo meter أو ال
+
+49
+00:04:11,810 --> 00:04:19,110
+spectrophotometer وبيعبرلي عن تركيز المحلول كيف
+
+50
+00:04:19,110 --> 00:04:26,140
+يعبر عن تركيز المحلول؟ ببساطة لإنهتركيز أي محلول
+
+51
+00:04:26,140 --> 00:04:31,320
+تمام هو عبارة عن function of its color تركيز أي
+
+52
+00:04:31,320 --> 00:04:36,220
+محلول هو يعني ب .. بيمكن التعبير عنه أو يمكن قياسه
+
+53
+00:04:36,220 --> 00:04:40,720
+من خلال شدة لونه فمثلا لو أنا حصلت على صبغة معينة
+
+54
+00:04:40,720 --> 00:04:46,020
+لنفرض أنه صبغة لونها أحمربلشت أخفف فيها طبعا؟ بلشت
+
+55
+00:04:46,020 --> 00:04:50,560
+أخفف فيها كل ما بعمل dilution أكتر أنا عم بقل ال
+
+56
+00:04:50,560 --> 00:04:55,220
+concentration عندي و كذلك تقل شدة اللون للمحلول
+
+57
+00:04:55,220 --> 00:05:01,860
+أعلى concentration هو أكثر شدة لللون و أخف
+
+58
+00:05:02,310 --> 00:05:05,370
+concentration أو أقل concentration هو أخف شدة
+
+59
+00:05:05,370 --> 00:05:10,470
+لللون تمام؟ إذا يتم التعبير عن تركيز المحاليل من
+
+60
+00:05:10,470 --> 00:05:17,590
+خلال لونها وأيضا للتالي يمكن قياس هذه المحاليل من
+
+61
+00:05:17,590 --> 00:05:22,930
+يعني من خلال تعبير عن درجة لونها وذلك بمرور الضوء
+
+62
+00:05:23,120 --> 00:05:27,800
+يعني كمية الضوء اللي يتلاشى في هذه المحلول و الضوء
+
+63
+00:05:27,800 --> 00:05:32,660
+اللي ينفذ عبر هذه المحلول يعبرلي بطريقة غير مباشرة
+
+64
+00:05:32,660 --> 00:05:37,080
+عن تركيز المحلول تمام؟ يعبرلي بطريقة غير مباشرة عن
+
+65
+00:05:37,080 --> 00:05:44,270
+تركيز المحلولفكرة أخرى، لو أنا جبت لمبة، لمبة ضوء
+
+66
+00:05:44,270 --> 00:05:49,570
+وحطيت أمامها قطعة زجاجية ملونة، هل كل الضوء اللي
+
+67
+00:05:49,570 --> 00:05:53,230
+موجود في ال light source، في المصدر، في اللمبة،
+
+68
+00:05:53,230 --> 00:05:56,310
+سينفذ إلى الجهة الأخرى من القطعة الزجاجية؟ بالطبع
+
+69
+00:05:56,310 --> 00:06:00,150
+لا، يعني لأ جزء من هذا الضوء سيتلاشى عبر القطعة
+
+70
+00:06:00,150 --> 00:06:05,950
+الزجاجية وجزء آخر سينفذ، فبالتالي لو كل ما كان لون
+
+71
+00:06:05,950 --> 00:06:11,820
+الزجاج داكن أكثر، غامق أكثرفبالتالي هيحجب لكمية
+
+72
+00:06:11,820 --> 00:06:16,180
+الضوء أكتر، تمام؟ وكمية الضوء اللي نعفد عبر هذه
+
+73
+00:06:16,180 --> 00:06:20,660
+القطعة الذجاجية هيكون أقل، تمام؟ فهذه هي فكرة
+
+74
+00:06:20,660 --> 00:06:27,280
+القياس .. التركيز تبع لشدة لونها و لمرور الضوء من
+
+75
+00:06:27,280 --> 00:06:27,780
+خلالها
+
+76
+00:06:35,350 --> 00:06:39,630
+طيب لنتحدث عن characteristics of light خواص الدوق
+
+77
+00:06:39,630 --> 00:06:44,230
+الدوق هو عبارة عن electromagnetic energy that
+
+78
+00:06:44,230 --> 00:06:48,830
+travels in waves هو عبارة عن طاقة كهرومغناطيسية
+
+79
+00:06:48,830 --> 00:06:53,930
+تسير في موجات ومانها تسير في موجات إذا الطول
+
+80
+00:06:53,930 --> 00:06:58,210
+الموجي لهذا الدوق هو عبارة عن the wavelength of
+
+81
+00:06:58,210 --> 00:07:03,410
+light is the distanceهو عبارة عن المسافة ما بين
+
+82
+00:07:03,410 --> 00:07:08,390
+two peaks two peaks يعني ما بين قمتين متتاليتين أو
+
+83
+00:07:08,390 --> 00:07:13,350
+قاعين متتالين أعلى نقطة في القمة بنسميها peak وأقل
+
+84
+00:07:13,350 --> 00:07:19,110
+نقطة في القاع أيضا بنسميها peak إذا بما أنه الطول
+
+85
+00:07:19,110 --> 00:07:22,230
+الموجي إذا بدنا نحكي عنه بنعبر عنه بالعربي هو
+
+86
+00:07:22,230 --> 00:07:27,940
+المسافة ما بينقمتين متتاليين أو قاعين متتاليين و
+
+87
+00:07:27,940 --> 00:07:31,020
+أعلى نقطة في القمة بنسميها beak و أعلى نقطة أو
+
+88
+00:07:31,020 --> 00:07:35,740
+أدنى نقطة في القاع بنسميه أيضًا beak إذا هو في
+
+89
+00:07:35,740 --> 00:07:39,540
+اللغة الإنجليزية نعرفه the distance between two
+
+90
+00:07:39,540 --> 00:07:44,920
+peaks of the light waveهذا الطول الموجي يتناسب
+
+91
+00:07:44,920 --> 00:07:50,620
+عكسيا مع طاقة الضوء إذا إذا كانت عندي ضوء بطول
+
+92
+00:07:50,620 --> 00:07:56,500
+موجي كبير هتكون طاقة هذا الضوء قليلة و العكس صحيح
+
+93
+00:07:56,500 --> 00:08:01,300
+إذا كان الطول الموجي قليل إذا طاقة هذا الضوء هتكون
+
+94
+00:08:01,300 --> 00:08:05,780
+عالية جدا، تمام؟ العلاقة عكسية ما بين الطول الموجي
+
+95
+00:08:05,780 --> 00:08:11,200
+وطاقة الضوءطيب أنا في عندي أنواع من الضوء في ضوء
+
+96
+00:08:11,200 --> 00:08:15,040
+مرئي، في ضوء أشعة فوق بنفسجية، أشعة تحت حمراء،
+
+97
+00:08:15,040 --> 00:08:18,660
+gamma، X-ray، radio waves في عندي أنواع كتير من
+
+98
+00:08:18,660 --> 00:08:24,220
+الأشعة الضوئية طيب لو كنا بنتحدث عن الأشعة المرئية
+
+99
+00:08:24,220 --> 00:08:27,780
+اللتي يمكن رؤيتها بالعين تمام؟ the visible region
+
+100
+00:08:27,780 --> 00:08:31,860
+ال visible region هي الأشعة المرئية تمام؟ هذه
+
+101
+00:08:31,860 --> 00:08:37,050
+الأشعة المرئية بيحكيلي إنه the color of lightis
+
+102
+00:08:37,050 --> 00:08:43,780
+function of its wavelengthلون الضوء عبارة عن دالة
+
+103
+00:08:43,780 --> 00:08:48,700
+أو يعبر عنه الطول الموجي طبعا، اللون اللي .. اللون
+
+104
+00:08:48,700 --> 00:08:53,160
+اللي الضوء يعبر عنه الطول الموجي هذا الطول الموجي
+
+105
+00:08:53,160 --> 00:08:58,540
+يزداد من اللون ال violet اللي هو أول لون في مرحلة
+
+106
+00:08:58,540 --> 00:09:03,080
+ال visible و بيظل يزيد باتجاه ال red color اللي هو
+
+107
+00:09:03,080 --> 00:09:06,700
+أخر لون في مرحلة ال visible كمان شوية راح نستعرض
+
+108
+00:09:06,700 --> 00:09:11,100
+جدول و راح نفهم أش يعني ال visible regionتمام؟
+
+109
+00:09:11,100 --> 00:09:14,660
+خلّينا بس ننتقل إلى النقطة الأخيرة اللي بتحكيلي
+
+110
+00:09:14,660 --> 00:09:18,300
+objects that appear colored absorb light at
+
+111
+00:09:18,300 --> 00:09:21,840
+particular wavelengths and reflect the other parts
+
+112
+00:09:21,840 --> 00:09:24,880
+at the visible spectrum resulting in many shades
+
+113
+00:09:24,880 --> 00:09:29,780
+of color إيش معنى هذه الجملة؟حكينا إنه المحلول
+
+114
+00:09:29,780 --> 00:09:35,580
+اللي يبدو ملونا تمام colored solution absorb light
+
+115
+00:09:35,580 --> 00:09:41,120
+يمتص الضوء على طول موجي معين ويعكس أو ينفذ من
+
+116
+00:09:41,120 --> 00:09:45,320
+خلاله الضوء الآخر أو ما تبقى the other parts ما
+
+117
+00:09:45,320 --> 00:09:53,140
+تبقى من هذا الضوءو مثل ما عرضنا من شوية في الصورة
+
+118
+00:09:53,140 --> 00:09:57,280
+مبدأ عمل هذا الجهاز بيحكيلي انه if a substance
+
+119
+00:09:57,280 --> 00:10:02,000
+تمام؟ if a substance can be converted to soluble
+
+120
+00:10:02,000 --> 00:10:06,440
+and colored material إذا كان في عندي محلول أو مادة
+
+121
+00:10:06,440 --> 00:10:11,020
+أو نتيجة تفاعل هي عبارة عن سولابل ذائبة وملونة
+
+122
+00:10:11,020 --> 00:10:16,000
+فممكن أنا أعمل determinationلل concentration لهذا
+
+123
+00:10:16,000 --> 00:10:19,440
+المحلول its concentration may be determined ممكن
+
+124
+00:10:19,440 --> 00:10:25,760
+اعرف التركيز من خلال كمية اللون اللي موجودة في
+
+125
+00:10:25,760 --> 00:10:30,180
+المحلول و هذا الكلام ايضا استعرضناه في صورة حكينا
+
+126
+00:10:30,180 --> 00:10:35,180
+انه كل ما زاد التركيز بتزيد شدة اللون طبعا كل ما
+
+127
+00:10:35,180 --> 00:10:40,880
+زاد التركيز بتزيد شدة اللون للمحلول لو جبنا صبغة و
+
+128
+00:10:40,880 --> 00:10:45,120
+الصبغة هدي لونها move مثلاتمام؟ وبلشت أخفف فيها
+
+129
+00:10:46,330 --> 00:10:50,510
+أخفف فيها باستخدام مثلا distilled water ماء طبعا
+
+130
+00:10:50,510 --> 00:10:54,650
+أضيف عليها ميه أكتر فأكتر فأكتر هلاحظ أنه تركيزها
+
+131
+00:10:54,650 --> 00:11:00,330
+بيقل مع ال dilution كل ما خففت أكتر التركيز بيقل
+
+132
+00:11:00,330 --> 00:11:08,410
+وبالتالي اللون بيقل طبعا فإذا دايما التركيز
+
+133
+00:11:08,410 --> 00:11:12,710
+ممكن أن اعمله determination من خلال شدة اللون لهذا
+
+134
+00:11:12,710 --> 00:11:16,550
+المحلولThe greatest sensitivity is obtained when
+
+135
+00:11:16,550 --> 00:11:19,370
+the light permitted to pass through the solution
+
+136
+00:11:19,370 --> 00:11:23,450
+of its particular wavelength طبعا عشان تكون هذه
+
+137
+00:11:23,450 --> 00:11:32,430
+الطريقة حساسة جدا يعني لقياس التركيز لابد يا بنات
+
+138
+00:11:32,430 --> 00:11:37,590
+أنه أنا أستخدم الطول الموجي اللي يحدث عنده أقصى
+
+139
+00:11:37,590 --> 00:11:43,670
+كمية امتصاص أقصى كمية امتصاصماذا يعني هذا الكلام؟
+
+140
+00:11:43,670 --> 00:11:47,690
+خلّينا بس نشوف الصورة اللي هنا هلأ أنا كان في عندي
+
+141
+00:11:47,690 --> 00:11:52,510
+light source تمام؟ لمبة بتعطيني أطوال موجية مختلفة
+
+142
+00:11:52,510 --> 00:11:57,870
+هذه الأطوال الموجية المختلفة لابد من إنه أنا أختار
+
+143
+00:11:57,870 --> 00:12:02,770
+طول موجي واحد فقط إنه اللي يعبر من خلال الإنبوبة
+
+144
+00:12:02,770 --> 00:12:08,350
+وجزء منه يتم انتصاصه و الجزء الآخر ينفذ يروح على
+
+145
+00:12:08,350 --> 00:12:11,710
+ال detector ال detector يقيس كمية الضوء اللي وصلته
+
+146
+00:12:11,920 --> 00:12:16,930
+يعرف ليه تركيز العينةطبعا طيب على أي أساس هذا
+
+147
+00:12:16,930 --> 00:12:20,450
+الطول الموجي الواحد اللي لازم أختاره من عدة أطوال
+
+148
+00:12:20,450 --> 00:12:25,070
+موجية موجودة في ال light source بنختاره طبعا لإنه
+
+149
+00:12:25,070 --> 00:12:31,610
+لازم يكون المحلول يمتصه أقصى امتصاص إذا الجزء
+
+150
+00:12:31,610 --> 00:12:35,870
+النافذ يكون أقل كمية يمكن نفادها عبر هذا المحلول
+
+151
+00:12:35,870 --> 00:12:39,730
+مادامه ممتص أكبر كمية ممكنة من المحلول إذا اللي
+
+152
+00:12:39,730 --> 00:12:45,860
+نافذ هنا هيكون أقل كمية من الضوءإذن بما إنه امتص
+
+153
+00:12:45,860 --> 00:12:50,860
+أكبر كمية من الضوء the maximum absorbance of
+
+154
+00:12:50,860 --> 00:12:55,320
+light، تمام؟ معناها اللي نفد هو أقل كمية يمكن أن
+
+155
+00:12:55,320 --> 00:12:59,600
+تنفذ من هذا الضوء، تمام؟ إذن لابد من إنه اختيار
+
+156
+00:12:59,600 --> 00:13:05,740
+طول موجي واحد ومحدد، اللي خواص إنه عنده المحلول
+
+157
+00:13:05,740 --> 00:13:10,040
+بيقوم بـthe maximum absorbance فبالتالي هيكون the
+
+158
+00:13:10,040 --> 00:13:14,630
+least أو the lowesttransmitted light طبعا the
+
+159
+00:13:14,630 --> 00:13:23,410
+lowest transmitted light طيب بالنسبة لأنواع
+
+160
+00:13:23,410 --> 00:13:27,430
+الطاقة الضوئية احنا حكينا انه في عندي اكثر من نوع
+
+161
+00:13:27,430 --> 00:13:32,330
+من ال .. الأشعة الضوئية gamma x-ray ultraviolet
+
+162
+00:13:32,330 --> 00:13:37,090
+visible infrared radio waves طبعا هنا بيكون اقل
+
+163
+00:13:37,090 --> 00:13:43,200
+طول موجي طبعاو هنا أعلى طول موجي، إذا هنا هيكون
+
+164
+00:13:43,200 --> 00:13:48,760
+أكثرها طاقة و هنا هيكون أقلها طاقة، ليش؟ لإن
+
+165
+00:13:48,760 --> 00:13:57,020
+العلاقة عكسية ما بين الطول الموجي و الطاقة
+
+166
+00:13:57,670 --> 00:14:02,910
+تمام؟ إذا زادت الطاقة يقل الطول الموجي، هيك بهذا
+
+167
+00:14:02,910 --> 00:14:06,310
+الاتجاه بيزيد الطول الموجي، هذا أقلها طول موجي و
+
+168
+00:14:06,310 --> 00:14:10,950
+هذا أكثرها طول موجي، هيك بتزيد الطاقة، هذه أقلها
+
+169
+00:14:10,950 --> 00:14:15,950
+طاقة و هذه أعلاها طاقة من نوع الأشعة، تمام؟ إحنا
+
+170
+00:14:15,950 --> 00:14:19,700
+حكينا في ال visibleتمام؟ هذه المنطقة تبدأ
+
+171
+00:14:19,700 --> 00:14:23,000
+بالviolet لإنه أصلا المنطقة اللي فوقها ultraviolet
+
+172
+00:14:23,000 --> 00:14:27,120
+ما فوق البنفسجية فبتبدا بالviolet و بتنتهي بال red
+
+173
+00:14:27,120 --> 00:14:30,560
+لإن المنطقة اللي بعدها infrared تحت حمرا فتدرج
+
+174
+00:14:30,560 --> 00:14:33,640
+الألوان بيكون بدايته في منطقة ال visible بدايته
+
+175
+00:14:33,640 --> 00:14:37,840
+violet و نهايته red طبعا الطول الموجي يزداد من
+
+176
+00:14:37,840 --> 00:14:41,500
+منطقة الviolet باتجاه من؟ باتجاه ال red لإن احنا
+
+177
+00:14:41,500 --> 00:14:46,490
+هيك حكينااتجاه زيادة الطول الموجي هذه أقلها طاقة
+
+178
+00:14:46,490 --> 00:14:53,310
+وهذه أقلها طول موجي وهذه أعلاها طول موجي تمام؟ بما
+
+179
+00:14:53,310 --> 00:14:56,790
+أن هذه أقلها طول موجي وهذه أعلاها طول موجي إذا
+
+180
+00:14:56,790 --> 00:14:59,830
+منطقة ال visible هيكون بدايتها أقل طول موجي
+
+181
+00:14:59,830 --> 00:15:03,250
+ونهايتها أعلى طول موجي طيب
+
+182
+00:15:05,220 --> 00:15:09,620
+the visible spectrum إيش معنى هذا الجدول؟ بحكيلي
+
+183
+00:15:09,620 --> 00:15:14,960
+لو تم محلول عندي، في عندي محلول مثلا بيمتص اللون
+
+184
+00:15:14,960 --> 00:15:18,540
+ال violet عند هذا الطول الموجي أقصى امتصاص، إذا
+
+185
+00:15:18,540 --> 00:15:22,020
+اللون ال .. الضوء اللي نافذ من خلال المحلول راح
+
+186
+00:15:22,020 --> 00:15:27,070
+يكون من green ليلة، تمام؟ من green ليلةو هي هنا في
+
+187
+00:15:27,070 --> 00:15:30,830
+عندي شريح ليها بالكلام حكالي if a substance that
+
+188
+00:15:30,830 --> 00:15:34,050
+absorbs violet إذا كان هي بتمتص اللون ال move على
+
+189
+00:15:34,050 --> 00:15:39,090
+طول موجي 400 مثلا هي ال range ما بين 400 ل450 هي
+
+190
+00:15:39,090 --> 00:15:43,290
+مثلا محلول بيمتص اللون ال violet على طول موجي 400
+
+191
+00:15:43,290 --> 00:15:48,130
+تمام طبعا إحنا لما نحدد طول موجي واحد يعني مش
+
+192
+00:15:48,130 --> 00:15:52,550
+range ما بنحدد range لحتى يصطدم بالعينة أو
+
+193
+00:15:52,550 --> 00:16:00,440
+بالمحلول بنحدد طول موجي واحدطيب إذا كان بينضس
+
+194
+00:16:00,440 --> 00:16:05,480
+اللون ال violet معناه it reflect هيعكس اللون اللي
+
+195
+00:16:05,480 --> 00:16:09,900
+.. او الضوء اللي أطوعله yellow ل green yellow ل
+
+196
+00:16:09,900 --> 00:16:12,800
+green to measure the concentration of a blue
+
+197
+00:16:12,800 --> 00:16:17,420
+solution تمام؟ إذا كان في عندي محلول لونه blueفهذا
+
+198
+00:16:17,420 --> 00:16:22,380
+المحلول اللي لونه blue أفضل طول موجي لحتى تنقيزه
+
+199
+00:16:22,380 --> 00:16:27,620
+عنده أو تنقيز تركيزه هو ال 590 590 يقع في هذا ال
+
+200
+00:16:27,620 --> 00:16:32,200
+range إذا هو أفضل امتصاص أو أفضل ضوء يمتصه هو
+
+201
+00:16:32,200 --> 00:16:37,120
+اللون ال yellow وينفذ من خلاله اللون ال blue اللي
+
+202
+00:16:37,120 --> 00:16:41,590
+بيروح على ال detectorفقط هي لحتى نشوف إنه كيف ..
+
+203
+00:16:41,590 --> 00:16:45,810
+إيش معناه كلمة إنه الضوء إنه أو المحلول لابد من
+
+204
+00:16:45,810 --> 00:16:52,370
+إنه نختارله طول موجي يحدث عنده أقصى امتصاص تمام؟
+
+205
+00:16:52,370 --> 00:16:57,600
+وأقل ال transmittedطيب خلينا نشوف مع بعض beers law
+
+206
+00:16:57,600 --> 00:17:01,660
+قانون beers اش يعني beers law او اش يعني قانون
+
+207
+00:17:01,660 --> 00:17:05,840
+beer؟ when a light of appropriate wavelength
+
+208
+00:17:05,840 --> 00:17:10,100
+strikes a cuvette that contain a colored sample
+
+209
+00:17:10,100 --> 00:17:13,500
+some of the light is absorbed and the rest is
+
+210
+00:17:13,500 --> 00:17:16,710
+transmitted through the sample to the detectorو
+
+211
+00:17:16,710 --> 00:17:21,270
+هذا الكلام تقريبا .. تقريبا احنا وضحنا لو كان في
+
+212
+00:17:21,270 --> 00:17:24,930
+عندي light source و هذا ال light source انا طبعا
+
+213
+00:17:24,930 --> 00:17:29,770
+بيعطيني أطوال موجية يعني عديدة تمام؟ أطوال موجية
+
+214
+00:17:29,770 --> 00:17:34,240
+عديدة هذا ال light source لمبة مثلا لمبة ضوءفاحنا
+
+215
+00:17:34,240 --> 00:17:38,440
+بنختار طول موجي واحد لحتى يخترق ال cuvette أش يعني
+
+216
+00:17:38,440 --> 00:17:43,260
+cuvette؟ ال cuvette معناها الوعاء أو الانبوبة اللي
+
+217
+00:17:43,260 --> 00:17:47,400
+بنحطها في داخل الجهاز و بيكون فيها المحلول الملون
+
+218
+00:17:47,400 --> 00:17:52,200
+المراد قياسهنتيجة ال reaction أو نتيجة فحص أو
+
+219
+00:17:52,200 --> 00:17:56,820
+محلول بدنا نعرف تركيزه فإذا بنحطه بالكيوفيت اللي
+
+220
+00:17:56,820 --> 00:18:01,560
+هي عبارة عن انبوبة تمام بهذا الشكل و بنحطها في
+
+221
+00:18:01,560 --> 00:18:04,860
+الجهاز تمام فإذا الضوء هذا اللي جاي من ال light
+
+222
+00:18:04,860 --> 00:18:09,800
+source و اللي احنا حكيناه طول موجي معينيحدث عنده
+
+223
+00:18:09,800 --> 00:18:14,280
+إنه المحلول يمطصه أقصى امتصاص طبعا مش .. مش أنا
+
+224
+00:18:14,280 --> 00:18:18,180
+range ما بدي قاعدة لعديد من الأطوار الموجية تختارق
+
+225
+00:18:18,180 --> 00:18:22,820
+المحلول، لأ أنا بدي طول موجي واحد فقط ليختارق
+
+226
+00:18:22,820 --> 00:18:27,820
+المحلولتمام؟ فإن جزء من هذا الضوء يتم امتصاصه راح
+
+227
+00:18:27,820 --> 00:18:32,720
+يصيرله absorbed قولنا يتلاشى أو يتم امتصاصه من
+
+228
+00:18:32,720 --> 00:18:37,920
+داخل المحلول تمام؟ و ال .. ال rest الباقي ما تبقى
+
+229
+00:18:37,920 --> 00:18:42,810
+من هذا الضوء تمام؟اش راح يصيرله؟ راح يصيرله
+
+230
+00:18:42,810 --> 00:18:47,690
+transmitted تمام؟ ينفذ، transmitted يعني ينفذ و
+
+231
+00:18:47,690 --> 00:18:51,090
+وين راح يروح؟ راح يروح على ال detector اللي موجود
+
+232
+00:18:51,090 --> 00:18:55,570
+في الجهاز اش هو هذا ال detector؟ هو ال photo cell
+
+233
+00:18:55,570 --> 00:19:00,390
+او ال photo multiplier tube اللي موجودة في الجهاز
+
+234
+00:19:00,390 --> 00:19:04,850
+و اللي ذكرناها بأول slideطبعا طيب لو جينا حسبنا
+
+235
+00:19:04,850 --> 00:19:07,590
+نسبة ال transmittance النسبة المقاوية لل
+
+236
+00:19:07,590 --> 00:19:11,510
+transmittance عن افترض ان الضوء اللي جاي من ال
+
+237
+00:19:11,510 --> 00:19:15,870
+light source هو ال I NOT شدة الضوء الابتدائية I
+
+238
+00:19:15,870 --> 00:19:22,850
+NOTونفترض إن الضوء اللي نفد من خلال الـ tube وذهب
+
+239
+00:19:22,850 --> 00:19:26,850
+إلى الـ detector هو الـ IT شدت الضوء لـ
+
+240
+00:19:26,850 --> 00:19:31,130
+transmittance اللي هو النافذ طيب معناها ال percent
+
+241
+00:19:31,130 --> 00:19:36,350
+of transmittance هي الـ IT على ال I note درمية في
+
+242
+00:19:36,350 --> 00:19:39,410
+المية زي قانون النسبة المئوية العادى اللي إحنا
+
+243
+00:19:39,410 --> 00:19:43,090
+بنستخدمه بحياتنا اليومية الجزء على الكل درمية في
+
+244
+00:19:43,090 --> 00:19:47,570
+المية هو هذه نسبة لـ transmittanceطيب، هل هذا ما
+
+245
+00:19:47,570 --> 00:19:53,170
+قالوا بيرز كله؟ لأ، بيرز حكالنا كمان إنهif the
+
+246
+00:19:53,170 --> 00:19:56,630
+concentration of solution is increased the I
+
+247
+00:19:56,630 --> 00:20:02,470
+transmitted will decrease and the transmitted
+
+248
+00:20:02,470 --> 00:20:06,170
+percentage is decreased إيش معنى هذا الكلام؟ خلينا
+
+249
+00:20:06,170 --> 00:20:10,870
+نشوف لو كان في عندي .. لو زاد تركيز هذا المحلول
+
+250
+00:20:10,870 --> 00:20:15,590
+إذا هتزيد شدة لونه، تمام؟ إذا هتزيد العتامة لهذا
+
+251
+00:20:15,590 --> 00:20:20,960
+المحلول، تمام؟ زادت شدة لونه، اتخيلي هل ..الضوء
+
+252
+00:20:20,960 --> 00:20:26,780
+سينفذ بشكل كبير من خلاله، بالعكس، إذا كل ما زادت
+
+253
+00:20:26,780 --> 00:20:32,200
+شدة اللون تمام؟ أو كل ما زادت تركيز هيزيد شدة
+
+254
+00:20:32,200 --> 00:20:41,540
+اللون، إذا هذا المحلول راح ينتص أو راح يعني يتلاشى
+
+255
+00:20:41,540 --> 00:20:47,860
+من خلاله أو يتلاشى فيهأكثر تمام كمية الضوء الممتص
+
+256
+00:20:47,860 --> 00:20:53,240
+من خلاله أكثر ال absorbance أعلى تمام إذا كمية
+
+257
+00:20:53,240 --> 00:20:58,020
+الضوء النافذة هتكون أقل إذا هناك علاقة عكسية ما
+
+258
+00:20:58,020 --> 00:21:01,940
+بين ال IT و نسبة ال transmittance و التركيز كل ما
+
+259
+00:21:01,940 --> 00:21:06,020
+زاد التركيز كل ما زاد اللون الضوء اللي هيمتص أعلى
+
+260
+00:21:06,020 --> 00:21:10,930
+إذاالـ transmittance هيكون أقل و هذا هو قانون
+
+261
+00:21:10,930 --> 00:21:16,010
+بيرز، بينص .. هو بيرز درس علاقة التركيز بالـ
+
+262
+00:21:16,010 --> 00:21:19,590
+transmitted light و عبّر عنه بهذه العلاقة حتى إن
+
+263
+00:21:19,590 --> 00:21:24,110
+العلاقة عكسية طبعا، العلاقة عكسية ما بين مين و
+
+264
+00:21:24,110 --> 00:21:29,230
+مين، ما بين التركيز و ال transmittanceحكى انه كل
+
+265
+00:21:29,230 --> 00:21:34,330
+ما زاد التركيز بيصير في عندي بتقل شدة الضوء
+
+266
+00:21:34,330 --> 00:21:37,170
+المنبعث اللي هو ال transmitted اه ال I transmitted
+
+267
+00:21:37,170 --> 00:21:41,130
+او ال I T وبالتالي نسبة الضوء المنبعث بالنسبة للكل
+
+268
+00:21:41,130 --> 00:21:45,730
+هتكون ايضا اقليلةthe relationship between the
+
+269
+00:21:45,730 --> 00:21:48,410
+concentration and transmitted is not linear
+
+270
+00:21:48,410 --> 00:21:52,090
+العلاقة مش linear ما بين التركيز و ال
+
+271
+00:21:52,090 --> 00:21:55,890
+transmittance احنا شوي حكينا العلاقة عكسية و كلمة
+
+272
+00:21:55,890 --> 00:21:59,590
+linear يعني علاقة خطية او علاقة تردية اذا the
+
+273
+00:21:59,590 --> 00:22:03,790
+relation between concentration and transmittance
+
+274
+00:22:03,790 --> 00:22:07,410
+is not linear ليست خطية و ليست تردية انما they are
+
+275
+00:22:07,410 --> 00:22:12,530
+universally proportional هم يتناسبوا تناسب عكسي
+
+276
+00:22:12,530 --> 00:22:20,550
+universallyطيبوالعلاقة هاي بيوضحها هذا ال curve
+
+277
+00:22:20,550 --> 00:22:25,330
+هلأ هاي هنا على ال y-axis نسبة ال transmittance
+
+278
+00:22:25,330 --> 00:22:30,630
+نسبة الضوء المنبعث أو النافذ تمام؟ وعلى ال x-axis
+
+279
+00:22:30,630 --> 00:22:35,750
+في عندى التركيز ال concentration طيب خلينا نشوف
+
+280
+00:22:35,750 --> 00:22:38,890
+هاي أقل تركيز هنا هتكون تمام؟ بالقرب من ال zero
+
+281
+00:22:38,890 --> 00:22:43,770
+أقل تركيز هاد الأقل من التركيز إيش؟ إلها .. إلها
+
+282
+00:22:43,770 --> 00:22:51,020
+أعلى transmittanceتمام؟ و أعلى تراكيز إيش إلها؟
+
+283
+00:22:51,020 --> 00:22:54,820
+إلها أقل transmittance تمام؟ إلها أقل
+
+284
+00:22:54,820 --> 00:22:58,620
+transmittance إذا العلاقة عكسية و لو جينا نربطها
+
+285
+00:22:58,620 --> 00:23:02,380
+منطقيا .. لو نيجي .. لو نيجي نربطها منطقيا نرجع
+
+286
+00:23:02,380 --> 00:23:06,570
+للصورة اللي فيها تدرج الألوان هذهأعلى تركيز يعني
+
+287
+00:23:06,570 --> 00:23:10,630
+أعلى شدة لون، لو مر الضوء كمية قليلة اللي هتنفذ من
+
+288
+00:23:10,630 --> 00:23:15,870
+خلاله، تمام؟ لكن أقل تركيز يعني أقل شدة لون، إذا
+
+289
+00:23:15,870 --> 00:23:20,170
+مر الضوء من خلاله كمية كبيرة من الضوء راح تنفذ،
+
+290
+00:23:20,170 --> 00:23:24,730
+تمام؟ لو أنا جيبت لمبة وحطيت أمامها زجاجة شفافةهذه
+
+291
+00:23:24,730 --> 00:23:29,050
+الزجاجة الشفاف راح تنفذ لكمية ضوء كبيرة طيب لو
+
+292
+00:23:29,050 --> 00:23:33,850
+وانت هذه الزجاجة بلون أبيض هينفذ كمية ضوء أقل طيب
+
+293
+00:23:33,850 --> 00:23:39,490
+لو وانت هذه الزجاجة اللي أمام اللامبا بلون رمادي
+
+294
+00:23:39,490 --> 00:23:43,630
+إذا هتحجب ضوء أكثر وكمية نوق الضوء النافذ هتكون
+
+295
+00:23:43,630 --> 00:23:49,200
+أقل طيب لو وانتها بلون أسود هيقل الضوءأكثر فأكثر
+
+296
+00:23:49,200 --> 00:23:53,440
+النافذ من خلال قطعة الزجاج إذا هذه هي العلاقة ما
+
+297
+00:23:53,440 --> 00:23:55,980
+بين شدة اللون كل ما زادت شدة اللون يعني زاد
+
+298
+00:23:55,980 --> 00:24:00,840
+التركيز كمية الضوء النافذ هتكون قليلة هتكون كمية
+
+299
+00:24:00,840 --> 00:24:04,500
+الضوء اللي نافذ أو المنبعث عبر ال tube ال
+
+300
+00:24:04,500 --> 00:24:07,960
+transmitted light هيكون إشماله قليل
+
+301
+00:24:10,670 --> 00:24:16,530
+طيب، ماذا تصبح العلاقة linear، ترضية، إذا أنا مثلت
+
+302
+00:24:16,530 --> 00:24:21,890
+على ال Y axis لسالي بلوجة T، مش نسبة ال T، سالي
+
+303
+00:24:21,890 --> 00:24:26,450
+بلوجة T، و هذا سالي بلوجة T بنعبر عنه بال A اللي
+
+304
+00:24:26,450 --> 00:24:33,690
+هو absorbance، تمام؟ absorbanceطيب احنا طبعا كمية
+
+305
+00:24:33,690 --> 00:24:37,990
+الضوء الممتص كل ما زاد التركيز بيزيد اللون إذا
+
+306
+00:24:37,990 --> 00:24:43,630
+هيزيد الامتصاص فإذا العلاقة linear و ترضية ما بين
+
+307
+00:24:43,630 --> 00:24:48,990
+التركيز و الامتصاص و العلاقة عكسية universally not
+
+308
+00:24:48,990 --> 00:24:55,940
+linear ما بين التركيز و ال transmittanceطيب بير
+
+309
+00:24:55,940 --> 00:25:01,140
+لامبرت لاو اجى لامبرت ويتعاون مع بير وقصاه قانون
+
+310
+00:25:01,140 --> 00:25:07,260
+اخر هذا القانون سموه a يساوي a في b في c اش يعني a
+
+311
+00:25:07,260 --> 00:25:11,340
+في b في c؟ خلينا بالاول ناخد نص القانون و بعدين
+
+312
+00:25:11,340 --> 00:25:16,000
+ناخد اللي هو التعبير الحسابي او الرياضي عن هذا
+
+313
+00:25:16,000 --> 00:25:21,300
+القانونBeer Lambert قانونهم بينص على أنه
+
+314
+00:25:21,300 --> 00:25:26,720
+الـabsorbance of solution is directly proportional
+
+315
+00:25:26,720 --> 00:25:30,000
+يعني العلاقة linear directly proportional يتناسب
+
+316
+00:25:30,000 --> 00:25:33,400
+ترضيا العلاقة linear with ash with the
+
+317
+00:25:33,400 --> 00:25:39,940
+concentration of substanceتمام؟ إذا بير لحاله درس
+
+318
+00:25:39,940 --> 00:25:43,980
+علاقة ال transmittance مع ال concentration و حكى
+
+319
+00:25:43,980 --> 00:25:50,340
+إنه العلاقة عكسية بير و Lambert درسوا التركيز و ال
+
+320
+00:25:50,340 --> 00:25:53,880
+.. ال concentration مع ال absorbance و حكوا إنه
+
+321
+00:25:53,880 --> 00:25:58,760
+العلاقة ترضية و هذا الكلام صار بديهي و صرنا بنعرفه
+
+322
+00:25:59,250 --> 00:26:02,750
+طيب التعبير الرياضي عبروا عن ال A capital بال
+
+323
+00:26:02,750 --> 00:26:06,190
+absorbance يعني هلا بدهم يدرسوا ال absorbance طبعا
+
+324
+00:26:06,190 --> 00:26:12,970
+فلازم يصيغوله تعبير رياضي غير سالب log Tفحكوا إن
+
+325
+00:26:12,970 --> 00:26:20,070
+هو يساوي a small b في c، تمام؟ طيب a هي عبارة عن
+
+326
+00:26:20,070 --> 00:26:25,310
+ال molar absorptivity coefficient و ال b هو عبارة
+
+327
+00:26:25,310 --> 00:26:30,550
+عن المسار الضوء ما بين ال light source و العينة
+
+328
+00:26:30,740 --> 00:26:35,500
+الضوء اللي ذي يقطعه في الجهاز الضوء المسار أو
+
+329
+00:26:35,500 --> 00:26:39,040
+المسافة اللي ذي يقطعها الدوء داخل الجهاز ما بين ال
+
+330
+00:26:39,040 --> 00:26:42,280
+light source اللمبة طبعة الضوء اللي بتزودني أطوار
+
+331
+00:26:42,280 --> 00:26:47,040
+موجية عديدة و ما بين ال .. ال .. المكان اللي أنا
+
+332
+00:26:47,040 --> 00:26:50,020
+بحط فيه ال cuvette الإنبوبة اللي فيها المحلول
+
+333
+00:26:50,020 --> 00:26:55,860
+الملون المراد قياس ترجزه تمام هذه هي BB إلها علاقة
+
+334
+00:26:55,860 --> 00:26:59,960
+بالجهاز وليس .. يعني أنا مابلعب فيها إذا هي ثابت
+
+335
+00:26:59,960 --> 00:27:05,120
+.. ثابت إله علاقة بالجهاز المستخدم A إلها علاقة
+
+336
+00:27:05,120 --> 00:27:07,380
+بنوع المادة المراد قياسها
+
+337
+00:27:10,330 --> 00:27:15,310
+احنا حكينا في البداية إنه إذا أنا بدي أقيص تركيز
+
+338
+00:27:15,310 --> 00:27:20,590
+مجهول في عينة لابد إني أقيصه بدلالة معلوم ألا وهو
+
+339
+00:27:20,590 --> 00:27:26,350
+ال standard، تمام؟ و مثلا لو أنا بدي أشتغل أو أبدي
+
+340
+00:27:26,350 --> 00:27:32,060
+أعمل فحص السكربدي أعمل فحص السكر حكينا إنه إذا
+
+341
+00:27:32,060 --> 00:27:35,200
+بتتذكروا محاضرة ال delution رسمتلكم two tubes
+
+342
+00:27:35,200 --> 00:27:39,800
+حكيتلكم أنا بجيب عينة أو tube فيها العينة و ال
+
+343
+00:27:39,800 --> 00:27:43,580
+reagent متفاعلين طبعا ال reagent بضيفه أو بضيف
+
+344
+00:27:43,580 --> 00:27:48,080
+العينة لل reagent فيحدث تغير تغير في اللون و أيضا
+
+345
+00:27:48,080 --> 00:27:51,440
+بضيف ال standard لل reagent و يحدث تغير في اللون
+
+346
+00:27:51,550 --> 00:27:55,830
+إذا كانت تغير في اللون الحادث في العينة نصف التغير
+
+347
+00:27:55,830 --> 00:27:58,570
+الحادث في ال standard إذا تركيزه نصف تركيز ال
+
+348
+00:27:58,570 --> 00:28:03,430
+standard وإذا كانت تغير الحادث في tube العينة ضعف
+
+349
+00:28:03,430 --> 00:28:11,420
+فإذا التركيز ضعف تركيز ال standardو هذا الكلام ال
+
+350
+00:28:11,420 --> 00:28:15,880
+.. النظري اللي احنا بنحكي ل .. لحتى ان نوصل فكرة
+
+351
+00:28:15,880 --> 00:28:20,220
+طب الكلام اللي عملي او الكلام اللي علمي ما .. اش
+
+352
+00:28:20,220 --> 00:28:25,620
+يعني؟ شوفوا معايا لو انا ضفت على reaction تعينة
+
+353
+00:28:25,620 --> 00:28:29,950
+طبعا عشان ا .. اقيس نسبة السكر في الدمرح يتفاعل ال
+
+354
+00:28:29,950 --> 00:28:34,550
+reagent مع السكر ويعطيني measurable change، تغير،
+
+355
+00:28:34,550 --> 00:28:39,190
+يمكن قياسه تغير في اللون فمثلا ممكن يتحول اللون
+
+356
+00:28:39,190 --> 00:28:43,150
+المحلول من colorless، من عديم اللون إلى لون pink،
+
+357
+00:28:43,150 --> 00:28:47,470
+ينتجلي مادةو اللون هذه .. المادة هاي لونها pink
+
+358
+00:28:47,470 --> 00:28:51,810
+تمام؟ فهذه هي اللي بدي أقيس ال .. ال .. ال
+
+359
+00:28:51,810 --> 00:28:55,710
+absorbance تبعها هي اللي أنا بدي أمرر ضوء من
+
+360
+00:28:55,710 --> 00:29:01,050
+خلالها و أشوف كام هي تنتص من الضوء و كام تنفذ
+
+361
+00:29:01,050 --> 00:29:05,890
+علشان يعبرلي كمية الضوء الممتص أو كمية الضوء
+
+362
+00:29:05,890 --> 00:29:08,810
+النافذ عن تركيزها، عن شدة لونها
+
+363
+00:29:16,970 --> 00:29:22,830
+طبعا يكون في عندي reagent هذا ال reagent أناباضيف
+
+364
+00:29:22,830 --> 00:29:28,730
+لإله standard و ال standard بيكون محلول من نفس نوع
+
+365
+00:29:28,730 --> 00:29:32,770
+الفحص يعني أنا بشتغل فحص سكر لازم يكون محلول سكري
+
+366
+00:29:32,770 --> 00:29:36,450
+معلوم التركيز بشتغل فحص protein لازم يكون محلولي
+
+367
+00:29:36,450 --> 00:29:40,170
+protein معلوم التركيز بشتغل فحص دهون لازم يكون
+
+368
+00:29:40,170 --> 00:29:44,910
+محلول دهون معلوم التركيز مابنفع أنه مثلا أكون
+
+369
+00:29:44,910 --> 00:29:49,170
+بشتغل فحص سكر للعينة و فحص مثلا .. او دهون لل
+
+370
+00:29:49,170 --> 00:29:54,370
+standard غلطغلط، لازم المادة اللي ناتجها الملونة
+
+371
+00:29:54,370 --> 00:29:59,150
+هتكون نفسها، تمام؟ المادة الملونة والناتج هتكون
+
+372
+00:29:59,150 --> 00:30:04,050
+نفسها علشان يكون لها نفس ال molar absorptivity
+
+373
+00:30:04,050 --> 00:30:08,810
+coefficient، تمام؟ طيب، طب لو أنا جيت .. طبقت هذا
+
+374
+00:30:08,810 --> 00:30:15,230
+القانون مرة لعينة مجهولة و مرة لل standard فهيو
+
+375
+00:30:15,230 --> 00:30:20,190
+مثلا عندي ال absorbanceلل X اللي هو المجهول يساوي
+
+376
+00:30:20,190 --> 00:30:25,050
+A في B في تركيز ال X اللي هو احنا ما بنعرفه ال
+
+377
+00:30:25,050 --> 00:30:27,530
+absorbance لل standard طبعا ال absorbance هدى
+
+378
+00:30:27,530 --> 00:30:30,550
+قراءات بيعطينيها الجهاز يعني هو اللى بيقيص كمية ال
+
+379
+00:30:30,550 --> 00:30:33,730
+absorbance كمية الضوء المتلاشى بيصلوا ل
+
+380
+00:30:33,730 --> 00:30:38,030
+transmittance على ال detector وبيطرحوا من ال .. ال
+
+381
+00:30:38,030 --> 00:30:42,150
+ضوء كمية الضوء الأساسية اللى جاية من ال source من
+
+382
+00:30:42,150 --> 00:30:47,030
+المصدر وبيعرفوا كمية ال absorbanceطمام؟ طيب فال
+
+383
+00:30:47,030 --> 00:30:50,590
+absorbance لل standard يساوي a في b في ال
+
+384
+00:30:50,590 --> 00:30:53,870
+concentration لل standard طب لو أنا جيت قسمت ال
+
+385
+00:30:53,870 --> 00:30:57,150
+absorbance لل standard لل x على ال absorbance لل
+
+386
+00:30:57,150 --> 00:31:02,830
+standard جيت قسمتهم على بعض مثل هيكabsorbance لل X
+
+387
+00:31:02,830 --> 00:31:06,850
+على absorbance لل standard طيب مش احنا حكينا انه
+
+388
+00:31:06,850 --> 00:31:11,110
+ال .. ال .. ال .. ال .. ال .. ال standard و ال ..
+
+389
+00:31:11,110 --> 00:31:15,850
+ال .. ال sample لازم يكونوا من نفس النوع و ينتجولي
+
+390
+00:31:15,850 --> 00:31:20,450
+بعد التفاعل نفس المادة و هيكونلها نفس ال A تمام
+
+391
+00:31:20,450 --> 00:31:24,070
+اللي هو ال molar absorptivity معامل الانتصاص لإلها
+
+392
+00:31:24,830 --> 00:31:29,590
+تمام إذا a عمّا a هتروح طيب و ال b مش هي واحدة
+
+393
+00:31:29,590 --> 00:31:32,350
+لإنه أنا بستخدم نفسي الجهاز و هي أصلاً إلها علاقة
+
+394
+00:31:32,350 --> 00:31:37,390
+بالجهاز و أنا مابغ .. يعني .. يعني صعب أ .. ألعب
+
+395
+00:31:37,390 --> 00:31:42,490
+فيها أو أغير فيها فإذا ..دي ايضا هتروح فاش هيضل
+
+396
+00:31:42,490 --> 00:31:48,370
+عندى ال absorbance ل ال test او ل ال unknown على
+
+397
+00:31:48,370 --> 00:31:52,190
+ال absorbance ل ال standard و يساوي تركيز ال test
+
+398
+00:31:52,190 --> 00:31:56,190
+او ال unknown او ال patient على تركيز ال standard
+
+399
+00:31:56,190 --> 00:31:59,750
+طيب مين اللى عندي مجهول؟ هدول بيعطيني اياهم الجهاز
+
+400
+00:31:59,750 --> 00:32:03,530
+و هذا معلومة تركيز معروف اذا تركيز ال unknown او
+
+401
+00:32:03,530 --> 00:32:07,230
+ال test او ال patient sample هو اللى مش معروفلو
+
+402
+00:32:07,230 --> 00:32:10,730
+أنا جيت طلعته في علاقة هيحكيلي ان هو عبارة عن ال
+
+403
+00:32:10,730 --> 00:32:13,610
+absorbance لل test او لل X على ال absorbance لل
+
+404
+00:32:13,610 --> 00:32:17,450
+standard درب تركيز ل standard تمام؟
+
+405
+00:32:20,050 --> 00:32:23,750
+طيب هناك requirements for the Beers Lambert Law to
+
+406
+00:32:23,750 --> 00:32:29,730
+hold true هناك لحتى ال Beers Lambert Law هاي قانون
+
+407
+00:32:29,730 --> 00:32:33,770
+Beero Lambert انهم .. انه يتم تطبيقه بطريقة صحيحة
+
+408
+00:32:33,770 --> 00:32:37,910
+و دقيقة لابد ان يكون في عندى شروط تتوفر في المحلي
+
+409
+00:32:37,910 --> 00:32:42,430
+للمستخدمة و شروط تتوفر في الجهاز المستخدم للقياسات
+
+410
+00:32:42,430 --> 00:32:46,110
+اول اشي solution requirement الشروط اللى .. الواجب
+
+411
+00:32:46,110 --> 00:32:53,440
+توفرها بال ..بالمحاليل لازم تكون the same نفس
+
+412
+00:32:53,440 --> 00:32:57,720
+المحاليل حكينا فحص السكر، السكر اللي موجود في
+
+413
+00:32:57,720 --> 00:33:02,220
+العينة مع ال reagent بيعطيلي مثلا مادة، المادة ده
+
+414
+00:33:02,220 --> 00:33:08,920
+دي لنفرض انها Y تمام؟ و لونها pinkلازم ال .. ال
+
+415
+00:33:08,920 --> 00:33:13,060
+standard يكون محلول سكري معلوم التركيز عشان لما
+
+416
+00:33:13,060 --> 00:33:17,260
+أضيفه مع ال reagent ال reagent يتفاعل و يعطيني نفس
+
+417
+00:33:17,260 --> 00:33:21,320
+المادة why اللي إلها نفس اللون pink تمام؟
+
+418
+00:33:23,350 --> 00:33:28,710
+والمادتين هدول اللي ده نتجوا إما في تيوب ال test
+
+419
+00:33:28,710 --> 00:33:33,670
+أو في تيوب ال standard اللي هم نفس ال A نفس معامل
+
+420
+00:33:33,670 --> 00:33:37,370
+الامتصاص تمام؟ اللي هم نفس ال small a اللي هو
+
+421
+00:33:37,370 --> 00:33:40,390
+معامل الامتصاص اللي هو ال molar absorptivity
+
+422
+00:33:40,390 --> 00:33:45,300
+coefficientطبعا يا بنات هذه ال .. ال .. ال إيه؟
+
+423
+00:33:45,300 --> 00:33:51,220
+تعتمد على نوع المادة و ليس تركيزها يعني مثلا الدوق
+
+424
+00:33:51,220 --> 00:33:55,840
+لما ينفذ خلال ماء غير ما ينفذ خلال زيت غير ما ينفذ
+
+425
+00:33:55,840 --> 00:33:59,220
+خلال خل أو خلال كحول أو خلال مادة لونها pink أو
+
+426
+00:33:59,220 --> 00:34:03,820
+خلال مادة لونها red أو مادة blue يعني بيعتمد على
+
+427
+00:34:03,820 --> 00:34:08,540
+نوع المادة و لونها و ليس على شدة لونها و تركيزها
+
+428
+00:34:08,540 --> 00:34:15,600
+تمام؟ طيبو أيضًا إذا اتفقنا ليش لازم يكونوا دسام
+
+429
+00:34:15,600 --> 00:34:19,960
+عشان يعطون نفس ال .. ال .. ال product نفس الناتج،
+
+430
+00:34:19,960 --> 00:34:24,880
+نفس اللون و بيتم طب أيضًا ال .. ال a تروح مع ال a
+
+431
+00:34:24,880 --> 00:34:30,510
+هنا و يتم تطبيق القانون بشكل صحيحتمام؟ و أيضًا
+
+432
+00:34:30,510 --> 00:34:36,750
+المادة الناتجة هي dy تكون ثابتة خلال فترة ال
+
+433
+00:34:36,750 --> 00:34:41,630
+reaction تظلها the same throughout the test تظلها
+
+434
+00:34:41,630 --> 00:34:46,790
+زي ما هي طول فترة الفحص إيش يعني؟ يعني not .. not
+
+435
+00:34:46,790 --> 00:34:51,630
+associate or dissociate يعني ما .. ما أشوفها ترثبت
+
+436
+00:34:51,870 --> 00:34:58,850
+تكسرت، تحللت، تبخرت، امتصت رطوبة، خفت، شدت اللون
+
+437
+00:34:58,850 --> 00:35:02,110
+فيها؟ لأ لازم تكون ثابتة طول فترة الفحص عشان انا
+
+438
+00:35:02,110 --> 00:35:05,530
+لما اخد قراءة على الجهاز الجهاز يعطيني قراءة
+
+439
+00:35:05,530 --> 00:35:10,190
+دقيقة، تمام؟ اذا لازم يكونوا the same و لازم
+
+440
+00:35:10,190 --> 00:35:14,070
+يكونوا ايضا the same throughout the test not
+
+441
+00:35:14,070 --> 00:35:20,130
+associate or dissociateأيضًا خلّيني ندكر كمان فكرة
+
+442
+00:35:20,130 --> 00:35:25,050
+تانية، اتخيلي لو أنا كان عندي عينة و العينة هذه تم
+
+443
+00:35:25,050 --> 00:35:28,950
+إضافتها إلى reagent علشان يتفاعل لي مع .. مع السكر
+
+444
+00:35:28,950 --> 00:35:32,810
+اللي فيها، مع ال glucose اللي فيها و يعطيني Y
+
+445
+00:35:32,810 --> 00:35:37,680
+product اللي لونه pinkلو أنا استخدمت different
+
+446
+00:35:37,680 --> 00:35:41,100
+standard مثلا يعني protein standard محلول ال
+
+447
+00:35:41,100 --> 00:35:45,100
+protein معلومة تركيزي و أضفته ل .. هذا ال standard
+
+448
+00:35:45,100 --> 00:35:48,620
+إلى .. إلى reagent السكر
+
+449
+00:35:50,560 --> 00:35:54,380
+معناها ال .. ال .. ال protein هذا ممكن يت .. ممكن
+
+450
+00:35:54,380 --> 00:35:58,640
+يتفاعل و ممكن لا يتفاعل مع reagent السكر فمثلا لو
+
+451
+00:35:58,640 --> 00:36:03,040
+تفاعل ممكن يعطيني مادة مش Y مادة Z لأنها move طب
+
+452
+00:36:03,040 --> 00:36:10,460
+أنا بنفع أقارن المادة Yفي العينة مجهولة التركيز
+
+453
+00:36:10,460 --> 00:36:15,920
+اللي لونها pink تمام في المادة Z اللي نتجت من تيوب
+
+454
+00:36:15,920 --> 00:36:20,100
+ال standard اللي لونها move غلط مابنفع عشان هيك
+
+455
+00:36:20,100 --> 00:36:23,260
+لازم يكونوا the same نفس المادة نفس التركيب و نفس
+
+456
+00:36:23,260 --> 00:36:26,740
+ال reaction يحدث عشان يعطيني نفس ال product و نفس
+
+457
+00:36:26,740 --> 00:36:30,860
+اللون بغض النظر عن شدته تمام لإنه ممكن التركيز ..
+
+458
+00:36:30,860 --> 00:36:33,960
+تركيز العينة يكون مختلف عن تركيز ال standard لازم
+
+459
+00:36:33,960 --> 00:36:38,530
+يعطيني نفس المادة نفس اللوننوعه نفس نوع اللون يعني
+
+460
+00:36:38,530 --> 00:36:43,430
+pink, mauve, blue, green, yellow هذه الألوان يعني
+
+461
+00:36:43,430 --> 00:36:47,550
+بس أنا بحكي على نوع اللون يعني اسم اللون تمام؟ و
+
+462
+00:36:47,550 --> 00:36:52,890
+لحتى أيضا يكون لهم نفس السبت a small اللي هو ال
+
+463
+00:36:52,890 --> 00:36:57,450
+coefficient of absorptivity of substance عشان يتم
+
+464
+00:36:57,450 --> 00:37:01,110
+تطبيق قانون له صحيح و يروح ال a مع ال a و يروح ال
+
+465
+00:37:01,110 --> 00:37:07,080
+b مع ال b و يتم تطبيق قانون Bear Lambert لهطيب إيه
+
+466
+00:37:07,080 --> 00:37:11,880
+instrument requirement؟الشروط الواجب توفرها في
+
+467
+00:37:11,880 --> 00:37:17,060
+الجهاز طبعا لازم يكون at satisfactory إله يعني
+
+468
+00:37:17,060 --> 00:37:21,380
+درجة مرضية من ال accuracy الدقة ال sensitivity
+
+469
+00:37:21,380 --> 00:37:25,520
+الحساسية and reproducibility منتج يعني ما .. يعني
+
+470
+00:37:25,520 --> 00:37:28,860
+ما بده يقرأ لعينتين تلاتة وبيطلف بده صيانة بده إيه
+
+471
+00:37:28,860 --> 00:37:33,740
+عدد calibration بده أرجع شغله مرة تانية بي ..
+
+472
+00:37:33,740 --> 00:37:39,620
+بيعلج مثلا أو إشي فهذه كل هالشغلات لازم يكون طبعا
+
+473
+00:37:39,850 --> 00:37:45,130
+بيشتغل بكفاءة عالية ده cubits الأنابيب اللي إحنا
+
+474
+00:37:45,130 --> 00:37:52,930
+بنستخدمهم لحتى يعني يتم إدراجهم داخل الجهاز و فيهم
+
+475
+00:37:52,930 --> 00:37:57,790
+المحلول الملون و يتم قياس عبرهم هادول لازم يكونوا
+
+476
+00:37:57,790 --> 00:38:02,840
+أول إشي cleanو أيضا free from scratches، إيش يعني
+
+477
+00:38:02,840 --> 00:38:08,580
+free from scratches؟ يعني خالي من الشقوق، الكثور،
+
+478
+00:38:08,580 --> 00:38:17,960
+الخدوش، الشطوبلازم تكون ملساق و تكون يعني سليمة ال
+
+479
+00:38:17,960 --> 00:38:21,240
+tube هاي مافيش فيها اي خدوش او شقوق او كسور لإن
+
+480
+00:38:21,240 --> 00:38:25,480
+هذا هيأثر على مسار الضوء من خلالها و جزء من الضوء
+
+481
+00:38:25,480 --> 00:38:32,180
+راح يتبدد بسبب انه هي مشققة او عليها كسور او خدوش
+
+482
+00:38:32,180 --> 00:38:38,600
+او لإنها مش نظيفة من برا فبالتالي هذا هيأثر على
+
+483
+00:38:38,770 --> 00:38:42,850
+كمية الضوء اللي هيقرأها الجهاز او يسجلها الجهاز
+
+484
+00:38:42,850 --> 00:38:49,090
+طيب لو انا جيت تصور التصور منطقي من ايش مكون
+
+485
+00:38:49,090 --> 00:38:53,590
+الجهاز لابد ان يكون مكون من light source مصدر
+
+486
+00:38:53,590 --> 00:38:58,130
+للضوء بيعطيني أطوال موجية معينة ولكن هناك طول موجي
+
+487
+00:38:58,130 --> 00:39:02,150
+واحد اللي يجب ان يخترق الى ال .. يعني او يصل الى
+
+488
+00:39:02,150 --> 00:39:04,830
+العينة او المحلول .. ال .. ال tube اللي فيها
+
+489
+00:39:04,830 --> 00:39:08,150
+العينة أو ال tube اللي فيها المحلولطبعا فلابد من
+
+490
+00:39:08,150 --> 00:39:11,310
+أنه يكون ما بين ال light source و ال sample holder
+
+491
+00:39:11,310 --> 00:39:16,890
+يكون في عندي wavelength selector تمام؟ و هو عازل
+
+492
+00:39:16,890 --> 00:39:22,230
+أو أدالي عازل طول موجي واحد ليعبر من خلال ال tube
+
+493
+00:39:22,230 --> 00:39:27,110
+ليش؟ لأن هذا الطول الموجي لابد أن يكون عليه أقصى
+
+494
+00:39:27,110 --> 00:39:32,530
+كمية امتصاص من المحلول المراد قياسهطبعا جزء من
+
+495
+00:39:32,530 --> 00:39:36,570
+الضوء سيتم امتصاصه و جزء سينفذ راح يروح الى ال
+
+496
+00:39:36,570 --> 00:39:40,130
+detector هذا ال detector راح يحول الطاقة الضوئية
+
+497
+00:39:40,130 --> 00:39:45,430
+اللى وصلته الى طاقة كهربائية و راح يطلع لي لابد ان
+
+498
+00:39:45,430 --> 00:39:49,070
+يكون في عندي شاشة او مكان اقرأ عليه النتيجة فراح
+
+499
+00:39:49,070 --> 00:39:52,630
+يكون في عندي read out مكان تطلع عليه النتيجة تبعتي
+
+500
+00:39:52,630 --> 00:39:58,720
+و النتيجة يا بنات بتكون عبارة عن شد الطيارمعبّر عن
+
+501
+00:39:58,720 --> 00:40:03,200
+كمية الضوء إما ال transmittance أو ال absorbance
+
+502
+00:40:03,200 --> 00:40:07,320
+طبعا؟ طب على أي أساس هو بيختار ليه .. يعطيني ال
+
+503
+00:40:07,320 --> 00:40:10,360
+transmittance أو ال absorbance؟ أنا بختار لما
+
+504
+00:40:10,360 --> 00:40:16,440
+ببرمج الجهاز أو لما بدي أجيأجهزه لقراءة فحص معين
+
+505
+00:40:16,440 --> 00:40:22,040
+أنا بختار هذا الطول الموجي اللي بده الواعي المحدد
+
+506
+00:40:22,040 --> 00:40:25,660
+اللي بده يصطدم بالعينة وانا بختار الجهاز يعطيني
+
+507
+00:40:25,660 --> 00:40:28,540
+قراءة Transmittance إذا Transmittance قعدوا ال bus
+
+508
+00:40:28,540 --> 00:40:31,920
+بده يحسب الضوء اللي يواصل لل detector يحولوا لطيار
+
+509
+00:40:31,920 --> 00:40:35,090
+كهربائي و يطلعوا على readoutإذا بدياه يقرألي
+
+510
+00:40:35,090 --> 00:40:37,790
+absorbance معناها هو بديه .. بديه يحسب ال
+
+511
+00:40:37,790 --> 00:40:41,910
+transmittance و يطرحه من الضوء الأساسي و يحسبلي
+
+512
+00:40:41,910 --> 00:40:46,450
+كمية ال absorbance و هذا ..ال absorbance هو اللي
+
+513
+00:40:46,450 --> 00:40:52,310
+راح يتحول لطيار كهربائي ويعطيني قيمته على ال
+
+514
+00:40:52,310 --> 00:40:55,950
+readout، اذا انا بحدد شغلتان، انا بحدد ال
+
+515
+00:40:55,950 --> 00:40:59,070
+wavelength، الطول الموجي المعين اللي يعزله ال
+
+516
+00:40:59,070 --> 00:41:02,530
+selector، وانا بحدد القراءة تظهر على هيئة
+
+517
+00:41:02,530 --> 00:41:06,820
+transmittance أو absorbanceتمام؟ طيب كيف ب .. بحدد
+
+518
+00:41:06,820 --> 00:41:11,540
+هذه الشركات و الفحوصات اللي بتجيني من الشركة
+
+519
+00:41:11,540 --> 00:41:15,640
+بيحكيلي والله فحص السكر يفضل انك انت تقرا ايه على
+
+520
+00:41:15,640 --> 00:41:20,400
+طول موجي خمسمية فحص الهوموجلوبين يفضل انك انت تقرا
+
+521
+00:41:20,400 --> 00:41:24,320
+ايه على طول موجي خمسمية واربعين فحسب لون الن ..
+
+522
+00:41:24,320 --> 00:41:29,240
+المحلول الناتج بعد التفاعلتمام؟ أو lonely product
+
+523
+00:41:29,240 --> 00:41:34,340
+اللي ناتج بيحدده الشركات الطول الموجي المناسب
+
+524
+00:41:34,340 --> 00:41:38,300
+لقراءة النتيجة عليه اللي بيكون عنده أقصى كمية
+
+525
+00:41:38,300 --> 00:41:44,670
+امتصاص وأقل كمية transmittance تمام؟إذن لازم يكون
+
+526
+00:41:44,670 --> 00:41:48,210
+في عندي stable source of radiation energy مصدر
+
+527
+00:41:48,210 --> 00:41:52,630
+ثابت للطاقة لازم يكون في عندي أداة that isolate
+
+528
+00:41:52,630 --> 00:41:55,950
+restricted region of the spectrum لازم يكون عندي
+
+529
+00:41:55,950 --> 00:42:04,310
+أداة تعزل restricted محدد أو يعني طول موجي محدد من
+
+530
+00:42:04,310 --> 00:42:08,210
+الطيف أو من الضوء transparent container هيكون في
+
+531
+00:42:08,210 --> 00:42:15,020
+عندي وعاءو شفاف هذا يوضع فيه المحلول الملون او
+
+532
+00:42:15,020 --> 00:42:18,740
+ناتج التفاعل او اللي هي نتائج الفحص على اللي يتم
+
+533
+00:42:18,740 --> 00:42:21,980
+قراءته لازم يكون في عندي detector و هذا ال
+
+534
+00:42:21,980 --> 00:42:25,420
+detector وظيفته انه يحول اللي هو ال radiation
+
+535
+00:42:25,420 --> 00:42:29,420
+energy او الطاقة الضوئية الى electrical energy الى
+
+536
+00:42:29,420 --> 00:42:34,860
+طاقة كهربائية و هذه كيمية التيار الكهربائي او شدة
+
+537
+00:42:34,860 --> 00:42:40,770
+التيار الكهربائي اللي بينتج من ال detectorبيظهر
+
+538
+00:42:40,770 --> 00:42:47,650
+على ال readout على شاشة أو يعني مكان مخصص لقراءة
+
+539
+00:42:47,650 --> 00:42:53,510
+النتائج تمام؟ إذا هذا هو تمثيل لمكونات الجهاز من
+
+540
+00:42:53,510 --> 00:42:58,150
+الداخل سواء كان photometry أو visible يعني UV
+
+541
+00:42:58,150 --> 00:43:02,650
+light photometry أو visible photometryطيب ال
+
+542
+00:43:02,650 --> 00:43:08,670
+slides القادمة رح تتحدث عن مكونات أو تفاصيل عن هذه
+
+543
+00:43:08,670 --> 00:43:12,550
+المكونات تفاصيل مكونات الجهاز نبدأ بال light
+
+544
+00:43:12,550 --> 00:43:17,990
+source هلأ في عندي أجهزة بيعني ال light source
+
+545
+00:43:17,990 --> 00:43:21,890
+مصدر الضوء بيعطيني أطوار موجية تقع في منطقة ال
+
+546
+00:43:21,890 --> 00:43:26,810
+ultraviolet احنا هنا ذكرناإنه في عندي مناطق أو
+
+547
+00:43:26,810 --> 00:43:31,490
+يعني regions من الطاقة الضوئية وكل طاقة ضوئية إلها
+
+548
+00:43:31,490 --> 00:43:36,370
+طول موجي معين في عندي أجهزة بتعطيني أطوار موجية
+
+549
+00:43:36,370 --> 00:43:40,190
+تقع في منطقة ال ultraviolet طب يعني ال ultraviolet
+
+550
+00:43:40,190 --> 00:43:45,880
+هدي إيش هيكون range الأطوار الموجية تبعتها؟من 160
+
+551
+00:43:45,880 --> 00:43:51,180
+ل 375 هذه اللمبة اللي بتعطيني أطوال موجية تقع في
+
+552
+00:43:51,180 --> 00:43:54,560
+منطقة ال ultraviolet اللي هو الأشعة فوق البنفسجية
+
+553
+00:43:54,560 --> 00:43:59,000
+و اللي بتعطيني أطوال موجية في range طبعا أطوال
+
+554
+00:43:59,000 --> 00:44:06,140
+موجية في range ما بين 160 ل 375 بتكون إما لمبة
+
+555
+00:44:06,140 --> 00:44:10,220
+Deuterium أو Hydrogen مصنوعة من ال Deuterium أو ال
+
+556
+00:44:10,220 --> 00:44:15,140
+Hydrogenتمام؟ إذا بتكون في عندي لمبة Deuterium أو
+
+557
+00:44:15,140 --> 00:44:19,760
+Hydrogen بتعطيني أطوال موجية معاقم يعني في range
+
+558
+00:44:19,760 --> 00:44:25,340
+ما بين 160 ل 375 و في النهاية ال selector بيعزللي
+
+559
+00:44:25,340 --> 00:44:29,480
+طول موجي واحد فقط ليصطدم بالعينة و بيكمل الشغل
+
+560
+00:44:29,480 --> 00:44:34,800
+تمام؟ في ال visible region إذا كان الجهاز بيعطيني
+
+561
+00:44:34,800 --> 00:44:40,250
+أو بيقرأ فقط في منطقة الضوء المرئيطمام؟ معناها
+
+562
+00:44:40,250 --> 00:44:44,210
+هيعطيني ال light source أطوار الموجية ما بين 350
+
+563
+00:44:44,210 --> 00:44:49,090
+ل2500 نانومتر و اللمبة اللي هتكون موجودة في الجهاز
+
+564
+00:44:49,090 --> 00:44:52,310
+هي تنجستن إذا في فحوصات تنقرأ على منطقة ال UV
+
+565
+00:44:52,310 --> 00:44:56,010
+light و في فحوصات تتنقرأ على ال visible ال UV
+
+566
+00:44:56,010 --> 00:45:01,190
+light بيكون الجهاز اللي بيقرأ الفحوصات هاي ال
+
+567
+00:45:01,190 --> 00:45:04,270
+light source تبعه إما Deuterium lamp أو hydrogen
+
+568
+00:45:04,270 --> 00:45:07,550
+lamp و الأطوار الموجية اللي بتوفرها هاي ال lampمن
+
+569
+00:45:07,550 --> 00:45:12,010
+160 ل 375 اما الفحوصات اللى بتقرا على ال visible
+
+570
+00:45:12,010 --> 00:45:18,090
+region ف .. او في ال visible photometer بيكون ال
+
+571
+00:45:18,090 --> 00:45:21,830
+.. الجهاز اللمبة طبعته عبارة عن tungsten و بتعطينى
+
+572
+00:45:21,830 --> 00:45:26,710
+أطوال موجية ما بين 350 ل 2500 نانومتر احنا عندنا
+
+573
+00:45:26,710 --> 00:45:31,550
+جهاز مكتوب عليه UV شرطة visible فى
+
+574
+00:45:31,550 --> 00:45:35,200
+spectrophotometerيعني بيعطيني أطواع الموجية في
+
+575
+00:45:35,200 --> 00:45:38,320
+منطقة ال ultraviolet و في ال visible الفحوصات اللي
+
+576
+00:45:38,320 --> 00:45:41,960
+بتنقرأ علي ال UV light ممكن أقرأها عليه و الفحوصات
+
+577
+00:45:41,960 --> 00:45:45,280
+اللي بتنقرأ علي ال visible أيضا بتنقرأ عليه إذا هو
+
+578
+00:45:45,280 --> 00:45:50,640
+هيزودلي أطواع الموجية ما بين 160 إلى 2500 نانومتر
+
+579
+00:45:50,640 --> 00:45:55,310
+و هيكون عنده lump نوعين أو two lumpsالـ Deuterium
+
+580
+00:45:55,310 --> 00:45:59,950
+و Hydrogen Lamp .. Deuterium او Hydrogen هذه لما
+
+581
+00:45:59,950 --> 00:46:03,590
+بدي اش .. لما بدي ادخله طول موجي في منطقة ال UV
+
+582
+00:46:03,590 --> 00:46:08,550
+مثلا 200 او 260 معناها هو هيحولها .. هيشغل ال
+
+583
+00:46:08,550 --> 00:46:12,150
+Deuterium او ال Hydrogen Lamp ويطيني الطول الموجي
+
+584
+00:46:12,150 --> 00:46:16,030
+اللي بديها طبعا؟ لو انا مثلا بدي اقرأ على 400 او
+
+585
+00:46:16,030 --> 00:46:19,670
+540 اللي هو مغلوب اني بنقرأ على 540 يعني على منطقة
+
+586
+00:46:19,670 --> 00:46:23,340
+ال visible طبعا؟معناها اللي هتشتغل ال tungsten
+
+587
+00:46:23,340 --> 00:46:28,060
+بيكون عنده two types of lamp و بيشغل واحدة منهم
+
+588
+00:46:28,060 --> 00:46:31,280
+حسب نوع الطول الموجي اللي أنا دخلته احنا حكينا
+
+589
+00:46:31,280 --> 00:46:34,900
+الطول الموجي انا بدخله و انا اللي بحدده و بيكون
+
+590
+00:46:34,900 --> 00:46:40,800
+الشركة اللي اعطتنيا تمام تبع الله الفحصطيب ال
+
+591
+00:46:40,800 --> 00:46:44,280
+wavelength selector هذا اللى بيعزلى طول موجى معين
+
+592
+00:46:44,280 --> 00:46:49,660
+اللى هو يتم ادخاله للجهاز هذا بيكون اما filter او
+
+593
+00:46:49,660 --> 00:46:55,520
+monochromator طبعا اذا كان filter فهو بيكون مصنوع
+
+594
+00:46:55,520 --> 00:46:59,960
+من طبقة transparent dielectric layer طبقة شفاف
+
+595
+00:46:59,960 --> 00:47:04,020
+مقطبة الها اقطاب ومصنوعة من ال calcium fluoride
+
+596
+00:47:04,020 --> 00:47:08,000
+وسمك هاى الطبقة هو اللى بيتحكم بالطول الموجى اللى
+
+597
+00:47:08,000 --> 00:47:13,930
+مار من خلالهاأو يعني إذا ما كان من ال transparent
+
+598
+00:47:13,930 --> 00:47:18,430
+dielectric layer of calcium chloride معناها بدي
+
+599
+00:47:18,430 --> 00:47:22,630
+يكون مصنوع من colored glass تمام؟ إذا calcium
+
+600
+00:47:22,630 --> 00:47:28,820
+fluoride layer أو colored glass هذا ال filterتمام؟
+
+601
+00:47:28,820 --> 00:47:31,740
+أو بيكون ال .. ال .. ال selector مش filter بيكون
+
+602
+00:47:31,740 --> 00:47:34,380
+monochromator إيش يعني monochromator؟ ال
+
+603
+00:47:34,380 --> 00:47:38,840
+monochromator هذا بيكون إما grating أو prism أنا
+
+604
+00:47:38,840 --> 00:47:43,560
+أقدر إنه المصطلحات هذه أول مرة ت .. تمر عليكم ولكن
+
+605
+00:47:43,560 --> 00:47:46,940
+إذا أنتم يعني بس .. يعني انتبهتوا للكلام اللي بحكي
+
+606
+00:47:46,940 --> 00:47:51,340
+هو فقط اللي مطلوب منكم و هيكون واضح بالنسبة لقلكم
+
+607
+00:47:51,340 --> 00:47:55,450
+إذا ال .. ال selector هذا ممكن يكون filterال
+
+608
+00:47:55,450 --> 00:47:58,910
+filter هذا ممكن يكون طبقة transparent، dielectric
+
+609
+00:47:58,910 --> 00:48:02,570
+layer مصنوعة من الكالسيمو فلورايد و سمك هذه الطبقة
+
+610
+00:48:02,570 --> 00:48:06,190
+هو اللي بيتحكم بالطول الموجي، كام طول موجي مر من
+
+611
+00:48:06,190 --> 00:48:13,220
+خلالها يعني طول الموجي يعني قيمته تمامأو .. أو إنه
+
+612
+00:48:13,220 --> 00:48:17,420
+يكون هذا ال filter مصنوع من الزجاج الملون و من
+
+613
+00:48:17,420 --> 00:48:22,060
+شوية ضربنا مثال حكينا الزجاج لو كان ملون و حطيته
+
+614
+00:48:22,060 --> 00:48:25,700
+أنا أمام لمبة هيحجز الضوء، هيحجب الضوء المارر،
+
+615
+00:48:25,700 --> 00:48:30,890
+تمام؟ يعني فكرة عمل filterطيب إذا كان ال .. ال
+
+616
+00:48:30,890 --> 00:48:33,510
+wavelength selected عبارة عن monochromator فبيكون
+
+617
+00:48:33,510 --> 00:48:37,250
+إما a grating أو a prism تمام؟ إيش يعني grating؟
+
+618
+00:48:37,250 --> 00:48:41,270
+ال grating هو عبارة عن قطعة زجاجية مشرشرة تمام؟
+
+619
+00:48:41,270 --> 00:48:47,050
+محففة، إلها حواف، إلها grooves تمام؟زي الزجاج و
+
+620
+00:48:47,050 --> 00:48:51,270
+هذه القطعة الزجاجية، الضوء بينعكس وينكسر، ينعكس
+
+621
+00:48:51,270 --> 00:48:55,530
+وينكسر عليها، بينعكس وبينكسر، عدة انكسارات و عدة
+
+622
+00:48:55,530 --> 00:49:01,210
+انعكاسات للضوء تؤدي إلى تحلله و بالتالي كل مرة
+
+623
+00:49:01,210 --> 00:49:06,750
+بيتحلل وبيتشتت فبيفقد جزء منهانكسر، انعكس، انكسر،
+
+624
+00:49:06,750 --> 00:49:11,410
+انعكس وانكسر وانعكس و هكذا على هذه الحواف تمام ال
+
+625
+00:49:11,410 --> 00:49:17,790
+.. ال .. ال mirror تمام فبالتالي هذه الحواف يعني
+
+626
+00:49:17,790 --> 00:49:22,370
+راح تتقضي إلى فقدان الدوء، فقدان ال spectrum
+
+627
+00:49:25,430 --> 00:49:29,970
+كمية الضوء اللى راح تنفذ من هذا ال selector هي
+
+628
+00:49:29,970 --> 00:49:34,730
+عبارة عن طول موجى واحد طيب إذا كان ال prism كلنا
+
+629
+00:49:34,730 --> 00:49:39,190
+بنعرف أنه ال prism المنشور الثلاثى بيحلل الأضواء
+
+630
+00:49:39,190 --> 00:49:45,130
+.. الضوء إلى أطياف تمام؟ إلى عدة أطياف فمثلا لو
+
+631
+00:49:45,130 --> 00:49:48,790
+كان في عندى هنا light source هذه العدسة بس عشان
+
+632
+00:49:48,790 --> 00:49:52,610
+تجمع للضوء و تمرره خلال ال prism ال prism هو ال
+
+633
+00:49:52,610 --> 00:49:53,550
+wavelength selector
+
+634
+00:49:56,140 --> 00:50:01,120
+طيب هذا الـ wavelength selector راح يحلل الضوء
+
+635
+00:50:02,820 --> 00:50:08,100
+تمام؟ و .. إلى عدة أطياف و بيكون في عندها حاجة
+
+636
+00:50:08,100 --> 00:50:13,660
+اسمها slit تمام؟ حاجة اسمها slit يعني زي جدار أو
+
+637
+00:50:13,660 --> 00:50:17,900
+حائط مفتوح متقوم هذا الجدار يتحرك أماما و يعني
+
+638
+00:50:17,900 --> 00:50:24,120
+بتحرك ال .. بت .. ب .. ساعدا و نزولا يعني لحتى إن
+
+639
+00:50:24,120 --> 00:50:28,120
+طيف واحد من هذه الأضواء هواللي بيعبر .. أي طفل
+
+640
+00:50:28,120 --> 00:50:30,980
+اللي هيعبر اللي أنا دخلته على الجهاز أثناء برمجته
+
+641
+00:50:30,980 --> 00:50:34,760
+أو أثناء إعداده لقراءة الفحص وعلى أي أساس تم
+
+642
+00:50:34,760 --> 00:50:39,360
+اختياره لإنه بيكون عنده أقصى كمية انتصاص المحلول
+
+643
+00:50:39,360 --> 00:50:43,180
+بينطست ضوء تمام؟ وأقل كمية transmittance بتروح لل
+
+644
+00:50:43,180 --> 00:50:46,680
+detector ال detector هيحول القراءة ل electric
+
+645
+00:50:46,680 --> 00:50:50,560
+current وهتروح على ال readout على ال digital
+
+646
+00:50:50,560 --> 00:50:55,350
+screenتمام؟ إذا كل من ال .. ال grading
+
+647
+00:50:55,350 --> 00:50:59,810
+monochromator و ال prism بيعتمد على تحلل الضوء
+
+648
+00:50:59,810 --> 00:51:04,690
+ولكن هنا عدة انكسارات و انعكسات للضوء تؤدي إلى
+
+649
+00:51:04,690 --> 00:51:09,870
+تشتتته و تحلله تمام؟ أما هنا مروره عبر ال prism
+
+650
+00:51:09,870 --> 00:51:13,930
+المنشور التلاتي راح يؤدي إلى تحلله إلى أطياف و في
+
+651
+00:51:13,930 --> 00:51:17,630
+عند سلك بتتحكم بأي ضوء أو أي طيف هو ال lemur طبعا
+
+652
+00:51:17,630 --> 00:51:21,450
+كل طيف بيكون له طول موجي معينحسب انا .. مين اللي
+
+653
+00:51:21,450 --> 00:51:25,250
+بدياه هو اللي بيعدي طيب
+
+654
+00:51:26,450 --> 00:51:30,210
+تختلف الأجهزة باحتواءها على ال .. ال wavelength
+
+655
+00:51:30,210 --> 00:51:34,990
+selector فجهاز ال photo meter بيكون ال selector
+
+656
+00:51:34,990 --> 00:51:39,950
+عبارة عن filter و اربطوها F F photo meter filter
+
+657
+00:51:39,950 --> 00:51:44,770
+أما جهاز ال spectrophotometer ال .. ال wavelength
+
+658
+00:51:44,770 --> 00:51:48,230
+selector عبارة عن monochromator تمام؟ هنا ال
+
+659
+00:51:48,230 --> 00:51:53,350
+filter إما calcium fluoride layer أوcolored glass
+
+660
+00:51:53,350 --> 00:51:58,130
+أما هنا ال filter أو ال wavelength selector عبارة
+
+661
+00:51:58,130 --> 00:52:01,990
+عن monochromator وهذا ال monochromator إما grating
+
+662
+00:52:01,990 --> 00:52:08,950
+monochromator أو a prism أصبح الأن في عندنافي
+
+663
+00:52:08,950 --> 00:52:14,090
+عندنا نوعين من الأجهزة وصيرنا بنفرق ما بين ال
+
+664
+00:52:14,090 --> 00:52:16,630
+photometer و ال spectrophotometer في البداية ال
+
+665
+00:52:16,630 --> 00:52:20,250
+chapter كنا بنحكي photometer و spectrophotometer
+
+666
+00:52:20,250 --> 00:52:24,210
+على أساس إن هم واحد و إن هم هم أجهزة فيهم detector
+
+667
+00:52:24,210 --> 00:52:27,650
+فيهم إما photocell أو photo multiplier tube
+
+668
+00:52:27,650 --> 00:52:30,790
+وبيقيصوا كمية الضوء اللي نافذة عبر المحلول الملون
+
+669
+00:52:30,790 --> 00:52:34,640
+لإلهمبقصول كمية اما transmittance او absorbance
+
+670
+00:52:34,640 --> 00:52:39,680
+تمام هذا كله كلام لا خلاف عليه ولكن يختلفوا في نوع
+
+671
+00:52:39,680 --> 00:52:43,080
+ال wavelength selector اللي موجود فيهم فال
+
+672
+00:52:43,080 --> 00:52:46,540
+photometer فيه filter يعني اما calcium fluoride او
+
+673
+00:52:46,540 --> 00:52:49,660
+color deglass و ال spectrophotometer فيه
+
+674
+00:52:49,660 --> 00:52:54,120
+monochromator يعني اما agrating او abrasive تمام؟
+
+675
+00:52:54,120 --> 00:53:00,000
+طيبsample container المعاقلة دي يحتوي على المحلول
+
+676
+00:53:00,000 --> 00:53:04,740
+الملون لكيوفيت مرت معانا لكيوفيت كتير وذكرناها
+
+677
+00:53:04,740 --> 00:53:08,620
+وفسرت إيش يعني كلمتي كيوفيت وفي المعمل أيضا راح
+
+678
+00:53:08,620 --> 00:53:12,480
+أورجيكم إياها و أحكيلكم تعليمات كيف إحنا نستخدمها
+
+679
+00:53:12,480 --> 00:53:17,290
+كيف أمسكها كيف نشتغل فيها فبالتاليhigh liquid هي
+
+680
+00:53:17,290 --> 00:53:21,530
+اللي that hold the sample بتحتوي .. هي عبارة عن ال
+
+681
+00:53:21,530 --> 00:53:26,190
+sample container بتحتوي ال sample المراد قياسها أو
+
+682
+00:53:26,190 --> 00:53:30,730
+المحلول المراد قياسه في الجهاز بتكون مصنوعة إما لل
+
+683
+00:53:30,730 --> 00:53:35,430
+glass أو لل quartz أو لل silicaالـ Silica تستخدم
+
+684
+00:53:35,430 --> 00:53:38,510
+في القراءات على الـ visible light و الـ glass
+
+685
+00:53:38,510 --> 00:53:43,710
+بتكون تستخدم للقراءات على ال UV light طبعا فإحنا
+
+686
+00:53:43,710 --> 00:53:49,490
+كل شغلنا معظم الشغل هذه الفترة هيكون على ال
+
+687
+00:53:49,490 --> 00:53:52,270
+visible فهتستخدموا ال Silica أو ال plastic
+
+688
+00:53:55,090 --> 00:53:59,450
+أما في .. ان شاء الله في معامل متقدمة هتاخدوا
+
+689
+00:53:59,450 --> 00:54:02,090
+فحوصات بتنقرع على ال UV light و هتلاحظوا أن
+
+690
+00:54:02,090 --> 00:54:07,130
+المستات بتطلعولكم equivitat خاصة من ال quarts أو
+
+691
+00:54:07,130 --> 00:54:11,150
+من ال glass لحتى تشتغلوا عليها تمام؟ على ال UV
+
+692
+00:54:11,150 --> 00:54:14,320
+lightطيب ال detector احنا حكينا ال detector اما
+
+693
+00:54:14,320 --> 00:54:17,700
+هيكون photo cell او photo multiplier tube هلأ
+
+694
+00:54:17,700 --> 00:54:20,380
+photo tube او photo cell نفس المعنى او photo
+
+695
+00:54:20,380 --> 00:54:23,620
+multiplier tube ال photo اللي بديكم تعرفوا او اللي
+
+696
+00:54:23,620 --> 00:54:27,320
+مطلوب منكم ان ال photo cell بتشتغل بطريقة ال photo
+
+697
+00:54:27,320 --> 00:54:31,100
+conductivityأما ال photo multiplier مبدأ عملها
+
+698
+00:54:31,100 --> 00:54:34,500
+بالأقطاب أقطاب السيليكون silicon diode electrode
+
+699
+00:54:34,500 --> 00:54:38,400
+هلأ كيف بالظبط و تفاصيل حق حول مصطلح ال photo
+
+700
+00:54:38,400 --> 00:54:42,500
+conductivity التوصيلية الضوئية أو تفاصيل حول ال
+
+701
+00:54:42,500 --> 00:54:45,800
+silicon diode electrode ما رح نخوض فيها كل اللي
+
+702
+00:54:45,800 --> 00:54:50,110
+مطلوب منكم انه ال photo tubesأو ال photo cells
+
+703
+00:54:50,110 --> 00:54:53,970
+بتعمل بمبدأ عمل ال photo conductivity و ال photo
+
+704
+00:54:53,970 --> 00:54:57,630
+multiplier tube بتعمل بمبدأ ال silicon diode
+
+705
+00:54:57,630 --> 00:55:02,370
+electrode تمام؟ و هذا ال detector حكينا أنه بتصلوا
+
+706
+00:55:02,370 --> 00:55:07,690
+الطاقة الضوئية من الضوء النافذ و بيحولها إلى طاقة
+
+707
+00:55:07,690 --> 00:55:11,510
+كهربائية إلى electrical energy عبر جهاز
+
+708
+00:55:11,510 --> 00:55:16,860
+galvanometer موجود داخل ال detector تمام؟سواء كان
+
+709
+00:55:16,860 --> 00:55:19,940
+photocell او photo multiplier تيوب خلونا بس نشوف
+
+710
+00:55:19,940 --> 00:55:24,240
+كيف هي تم تحويل الطاقة الضوئية إلى طاقة كهربائية
+
+711
+00:55:24,240 --> 00:55:28,960
+هلأ اللي بيصير انه الضوء اللي نافذ عبر المحلول ل
+
+712
+00:55:28,960 --> 00:55:32,580
+transmittance رح يصل ال detector اول ما طبعا ال
+
+713
+00:55:32,580 --> 00:55:35,960
+detector فيه cathode وفيه anode قطب موجب و قطب
+
+714
+00:55:35,960 --> 00:55:41,940
+سالمهذا الضوء راح يصطدم بالقطب السالب، هو فوتونات،
+
+715
+00:55:41,940 --> 00:55:45,620
+تمام؟ هذه الفوتونات راح تصطدم بالقطب السالب اللي
+
+716
+00:55:45,620 --> 00:55:49,560
+هو الـ cathode فراح يتحرر من هذا ال cathode
+
+717
+00:55:49,560 --> 00:55:55,020
+إلكترونات، تمام؟ و هذه الإلكترونات راح تتجه باتجاه
+
+718
+00:55:55,020 --> 00:55:59,340
+الأعناض، القطب الموجب، تمام؟ هيها، هذا اتجاهها
+
+719
+00:55:59,340 --> 00:56:05,800
+للإلكترونات، بالاتجاه القطب الموجبتمام؟ طيب سرايان
+
+720
+00:56:05,800 --> 00:56:10,500
+إلكترونات أو شحنات سالبة من القطب السالب إلى القطب
+
+721
+00:56:10,500 --> 00:56:14,720
+الموجب هذا معناه إنه في عندي دارة كهربائية يمر
+
+722
+00:56:14,720 --> 00:56:19,300
+فيها طيار تمام؟ جهاز ال galvanometer رح يقيصلي
+
+723
+00:56:19,300 --> 00:56:27,370
+كمية شدة الطيار اللي نشأتنشأت نتيجة إيش؟ نتيجة
+
+724
+00:56:27,370 --> 00:56:33,570
+انبعاث ضوء عبر ال tube المحلول الملون نفذ من خلاله
+
+725
+00:56:33,570 --> 00:56:39,250
+هذا الضوء ضرب في ال cathode و اتحرر الإلكترونات
+
+726
+00:56:39,250 --> 00:56:42,510
+هاي الإلكترونات انتقلت من القطب السالب إلى القطب
+
+727
+00:56:42,510 --> 00:56:47,170
+الموجب إذا انتي عندى ضوء photonوكمية transmitted
+
+728
+00:56:47,170 --> 00:56:51,250
+light راحت إلى ال cathode حررت إلكترونات
+
+729
+00:56:51,250 --> 00:56:56,490
+الإلكترونات هذه تحركت إلى ال anode بتكون عندي طيار
+
+730
+00:56:56,490 --> 00:57:00,990
+شدت الطيار تقاس بال galvanometer و راح تظهر على ال
+
+731
+00:57:00,990 --> 00:57:04,930
+readout إما ك transmittance كقيمة transmittance أو
+
+732
+00:57:04,930 --> 00:57:09,610
+راح تظهر كقيمة absorbance مين اللي بيحدد أن الجهاز
+
+733
+00:57:09,610 --> 00:57:12,910
+يعمل حسابته و يقيص لل absorbance أو يقيص لل
+
+734
+00:57:12,910 --> 00:57:17,360
+transmittance directlyأنا اللي ب .. ب .. بدخل ال
+
+735
+00:57:17,360 --> 00:57:20,880
+mood of read يعني أنت إيش تقرألي يا جهاز؟ تقرألي
+
+736
+00:57:20,880 --> 00:57:24,680
+absorbance ولا تقرألي transmittance؟ وكل تعاملاتنا
+
+737
+00:57:24,680 --> 00:57:28,800
+أو معظم تعاملاتنا بتكون يا بنات بال absorbance و
+
+738
+00:57:28,800 --> 00:57:34,970
+ليس بال transmittance، تمام؟ طيببالنسبة لجهاز آخر
+
+739
+00:57:34,970 --> 00:57:38,930
+تمام اسمه flame photometry أو flame emission
+
+740
+00:57:38,930 --> 00:57:43,450
+spectroscopy هو جهاز بيشتغل نفس مبدأ عمل لـ
+
+741
+00:57:43,450 --> 00:57:47,350
+spectrophotometer flame photometry is a spectral
+
+742
+00:57:47,350 --> 00:57:51,130
+method in which excitation is caused by spraying a
+
+743
+00:57:51,130 --> 00:57:54,970
+solution of the sample in a hot flame طبعا هو
+
+744
+00:57:54,970 --> 00:57:59,640
+عبارة عنالجهاز بيشتغل برضه كمان ب spectral method
+
+745
+00:57:59,640 --> 00:58:04,100
+بطريقة قياس type الضوء او كمية الضوء من خلال انه
+
+746
+00:58:04,100 --> 00:58:09,320
+انا بعمل spraying للعينة على لهب اذا مصدر الضوء
+
+747
+00:58:09,320 --> 00:58:14,300
+هنا ال light source لهب و ليس لمبةتمام؟ هذا
+
+748
+00:58:14,300 --> 00:58:18,240
+الإختلاف و characteristic radiation is emitted in
+
+749
+00:58:18,240 --> 00:58:21,360
+flame by individual element and the emission
+
+750
+00:58:21,360 --> 00:58:24,800
+intensity is proportional to the concentration of
+
+751
+00:58:24,800 --> 00:58:28,660
+the element introduced into the flame شوفوا معايا
+
+752
+00:58:29,370 --> 00:58:34,970
+هلأ ال .. ال .. كمية الضوء اللي بينبعث من هذا
+
+753
+00:58:34,970 --> 00:58:42,470
+اللهب بتتناسب تناسبا طرضيا مع تركيز المواد اللي
+
+754
+00:58:42,470 --> 00:58:47,310
+موجودة في العينة هذا الجهاز بيستخدم لقياس ال
+
+755
+00:58:47,310 --> 00:58:50,850
+electrolytes في العينة، في عينات الدم أو سواء
+
+756
+00:58:50,850 --> 00:58:55,560
+الجسم تمام؟ إيش يعني electrolytes؟يعني الأيونات
+
+757
+00:58:55,560 --> 00:58:58,540
+Sodium, Potassium, Calcium, Magnesium, Fluoride,
+
+758
+00:58:58,640 --> 00:59:03,560
+Chloride, Sodium، هاي كلها عبارة عن أيونات يتم
+
+759
+00:59:03,560 --> 00:59:08,760
+قياسها بطريقة الفيلم فوتومتري أو الفيلم Emission
+
+760
+00:59:08,760 --> 00:59:12,980
+Spectroscopyماذا يحدث في هذا الجهاز؟ اول اشي انا
+
+761
+00:59:12,980 --> 00:59:17,400
+مافي عنده cuvette يعني انا مابدخل ل tube او يعني
+
+762
+00:59:17,400 --> 00:59:21,840
+المحلول في tube cuvette ويداخل الجهاز الجهاز يتلقى
+
+763
+00:59:21,840 --> 00:59:25,680
+العينة بطريقة ال suction اذا في عنده زي suction زي
+
+764
+00:59:25,680 --> 00:59:34,140
+..أداة أو جزء بينطس العينة امتصاص تمام؟ و هذه
+
+765
+00:59:34,140 --> 00:59:38,280
+العينة اللي يتم امتصاصها بتدخل إلى حجرة داخل
+
+766
+00:59:38,280 --> 00:59:44,820
+الجهاز مكان صغير مخصص للعينة هذه الحجرة درجة
+
+767
+00:59:44,820 --> 00:59:49,860
+حرارتها عالية جدا لإنه يعني موجود في أعلاها إفلام
+
+768
+00:59:49,860 --> 00:59:55,740
+لهبطبعا طيب نتيجة درجة الحرارة العالية هذه العينة
+
+769
+00:59:55,740 --> 01:00:01,440
+السائلة سواء blood أو سواء الجسم راح تتحول من ..
+
+770
+01:00:01,440 --> 01:00:06,920
+من حالة سائلة إلى حالة ما بين السائلة والغازية
+
+771
+01:00:06,920 --> 01:00:12,460
+اللي هي ال spray راح تصير رذاذ طبعا هذا الرذاذ
+
+772
+01:00:12,460 --> 01:00:19,060
+يتصعد و .. يعني يتصعد باتجاه ال flame باتجاه اللهب
+
+773
+01:00:19,470 --> 01:00:27,630
+إذا تساعد باتجاه اللهب إذا تمام إذا رح يغيرلي خواص
+
+774
+01:00:27,630 --> 01:00:33,870
+اللهب رح يغير لون اللهب تمام إذا عينة يتم إمتصاصها
+
+775
+01:00:33,870 --> 01:00:38,410
+بتروح على حجرة هذه الحجرة اسمها nebulizer هذه
+
+776
+01:00:38,410 --> 01:00:42,870
+الحجرة طبعا درجة حررتها عالية لإن في عندي على
+
+777
+01:00:42,870 --> 01:00:50,080
+أعلاها أو على سقفها فيلهب فهي العينة اللي دخلت لل
+
+778
+01:00:50,080 --> 01:00:55,940
+nebulizer بتتحول من حالة سائلة إلى حالة ما بين
+
+779
+01:00:55,940 --> 01:01:00,060
+السائلة والغازية اللي هي حالة ال spray بتصير رذاذ
+
+780
+01:01:00,060 --> 01:01:07,220
+الرذاذ هذا يتصاعد وطبعا بيأثر على خواص اللهبطمام؟
+
+781
+01:01:07,220 --> 01:01:11,740
+هذا خواص اللهب مثلا لو انا كنت عارفة انه ال sodium
+
+782
+01:01:11,740 --> 01:01:16,660
+بيعطيني اللهب لونه أحمر نفترض مثلا انه اشتعال ال
+
+783
+01:01:16,660 --> 01:01:19,840
+sodium هيعطيني لون أحمر هيغير لون اللهب للون
+
+784
+01:01:19,840 --> 01:01:25,240
+الأحمر اذا في عندي أشعة هنا بتجمع اللون الأحمر
+
+785
+01:01:25,240 --> 01:01:29,820
+كمية اللون الأحمر اللي موجودة بتتجمع و بتعبرخ من
+
+786
+01:01:29,820 --> 01:01:33,220
+خلال filter ال filter هذا اوزميت الضوء بشكل عام
+
+787
+01:01:33,220 --> 01:01:36,600
+اللى جاي من اللهب بيتجمع و بيروح على ال filter ال
+
+788
+01:01:36,600 --> 01:01:40,140
+filter هيفوت لفقط الضوء اللون الأحمر فهيروح على ال
+
+789
+01:01:40,140 --> 01:01:43,980
+photo detector و يتحول الضوء إلى طاقة كهربائية و
+
+790
+01:01:43,980 --> 01:01:46,940
+بتطلع على ال readout هنا ال readout اللى بيطلع
+
+791
+01:01:46,940 --> 01:01:52,440
+بيتناسب تناسبان طرديا مع ان هو emitted تمام؟ ولكن
+
+792
+01:01:52,440 --> 01:01:57,540
+يتناسب تناسبان طرديا مع التركيزعلى كام ال sodium
+
+793
+01:01:57,540 --> 01:02:01,580
+اللي موجود هيعطيني لها بأحمر شديد إذا كمية القراءة
+
+794
+01:02:01,580 --> 01:02:05,320
+اللي نتجت بتاع التناسب ترضيا هتكون كمية الضوء اللي
+
+795
+01:02:05,320 --> 01:02:09,940
+وصل لل detector هيكون كبير فبالتالي بيتعبر لتناسب
+
+796
+01:02:09,940 --> 01:02:14,340
+ترضي مع ال sodiumنفترض اشتعالي البوتاسيوم بينتج
+
+797
+01:02:14,340 --> 01:02:19,400
+عنه لون أزرق، إذا هيتغير خواص اللهب و يصير فيه لون
+
+798
+01:02:19,400 --> 01:02:25,600
+أزرق، فالقشعة، العدسة الجامعة هاي بتجمعلي الضوء
+
+799
+01:02:25,600 --> 01:02:28,500
+كله ككل، و بتفوته على ال filter، ال filter فقط
+
+800
+01:02:28,500 --> 01:02:31,420
+بعزل اللون الأزرقبيفوتوا .. بيفوتوا على ال photo
+
+801
+01:02:31,420 --> 01:02:35,380
+detector اللي هو ال detector فبالتالي بيحول الطاقة
+
+802
+01:02:35,380 --> 01:02:37,820
+الضوئية اللي أجته من اللون الأزرق إلى طاقة
+
+803
+01:02:37,820 --> 01:02:40,900
+كهربائية و بتطلع على ال read out كمية الطاقة اللي
+
+804
+01:02:40,900 --> 01:02:43,540
+طلعت أو القراءة اللي طلعت كل ما كانت أعلى يعني
+
+805
+01:02:43,540 --> 01:02:47,360
+كمية الضوء أعلى يعني هنا صار في عندي لون أزرق كتير
+
+806
+01:02:47,360 --> 01:02:51,680
+إذا في عندي مثلا potassium اشتعلي كتير فهذه علاقة
+
+807
+01:02:51,680 --> 01:02:57,410
+حكينا علاقة ترضية على عكس مثلاإنه في ال
+
+808
+01:02:57,410 --> 01:02:59,810
+spectrophotometer حكينا في ال spectrophotometer
+
+809
+01:02:59,810 --> 01:03:01,910
+كمية الضوء اللي واصل لل detector هو ال
+
+810
+01:03:01,910 --> 01:03:07,950
+transmittance ويتناسب تناسبان عكسي مع العينة مع
+
+811
+01:03:07,950 --> 01:03:10,990
+تركيز العينة إنما ال absorbance اللي تبدأت في
+
+812
+01:03:10,990 --> 01:03:14,430
+العينة هو اللي يتناسب تناسبان ترضيا مع العينة
+
+813
+01:03:14,430 --> 01:03:18,070
+تمام؟ فحلو إنه اتفرقوا و تربطوا الأمور ببعضها
+
+814
+01:03:18,070 --> 01:03:23,230
+البعض لحتى تعمقوا الفهم عندكم هيك بنكون وصلنا
+
+815
+01:03:23,230 --> 01:03:26,860
+لنهاية محاضرتنا لليوم يعطيكم ألف عافيةالسلام عليكم
+
diff --git a/PL9fwy3NUQKwY9jl8xw-bWkVZkWcGGyspd/xD3hYPD-Pa0.srt b/PL9fwy3NUQKwY9jl8xw-bWkVZkWcGGyspd/xD3hYPD-Pa0.srt
new file mode 100644
index 0000000000000000000000000000000000000000..f67b5c2de808d89135b6556ccd648731e896e285
--- /dev/null
+++ b/PL9fwy3NUQKwY9jl8xw-bWkVZkWcGGyspd/xD3hYPD-Pa0.srt
@@ -0,0 +1,1138 @@
+1
+00:00:03,740 --> 00:00:07,540
+السلام عليكم ورحمة الله وبركاته أهلا وسهلا بكم
+
+2
+00:00:07,540 --> 00:00:10,260
+عزيزاتي طالباتي نفس التواتر الثاني من قسم العلم
+
+3
+00:00:10,260 --> 00:00:15,360
+الطبية المخبرية أنا أسجل هذه المحاضرة للمرة
+
+4
+00:00:15,360 --> 00:00:20,440
+الثانية بعد ما جزء من الطالبات أو عدد من الطالبات
+
+5
+00:00:20,440 --> 00:00:25,520
+اشتكى أن في نص الـ record أو في نص التسجيل يروح
+
+6
+00:00:25,520 --> 00:00:29,420
+...بتروح الصورة فطلبت من البعض أن يتبعوا مع...
+
+7
+00:00:29,420 --> 00:00:32,740
+يعني يفتحوا ملف الـ Word زي ما أنا فاتحته هكذا
+
+8
+00:00:32,740 --> 00:00:36,440
+يتبعوا معي على كلامي بس يبدو أنه لسه كانت المشكلة
+
+9
+00:00:36,440 --> 00:00:43,250
+قائمة و مش... يعني كبداية لسه ما عارفين ف... حابة
+
+10
+00:00:43,250 --> 00:00:46,110
+يعني خلاص بالآخر قررت أن أسجل هذه المحاضرة
+
+11
+00:00:46,110 --> 00:00:50,470
+مرة ثانية أولا أهلا وسهلا بكم وحكينا
+
+12
+00:00:50,470 --> 00:00:54,650
+لكم أهلا وسهلا بكم في القسم وأن يعني كان بودنا
+
+13
+00:00:54,650 --> 00:00:59,350
+أن نشوفكم face to face ونلتقي بكم ونعرفكم عن
+
+14
+00:00:59,350 --> 00:01:03,410
+القسم، بس يعني هذه الأزمة حالت دون ذلك، و...ولكن
+
+15
+00:01:03,410 --> 00:01:06,570
+إن شاء الله يعني كلنا أمل أن تتجاوز الأزمة
+
+16
+00:01:06,570 --> 00:01:11,070
+على خير بأقل الخسائر، ونرجع لمختبراتنا ونرجع لـ
+
+17
+00:01:11,070 --> 00:01:15,230
+...القسم ونرجع نشوفكم ونقرب إن شاء الله ولعله خير
+
+18
+00:01:15,230 --> 00:01:19,270
+يعني نأمل ذلك، وراح نكون مع بعض إن شاء الله
+
+19
+00:01:19,270 --> 00:01:25,030
+بمساق مقدمة في العلوم الطبية المخبرية أو مقدمة في
+
+20
+00:01:25,030 --> 00:01:30,130
+التحليل الطبي، اللي هي عبارة عن مساق تأسيسي و
+
+21
+00:01:30,130 --> 00:01:35,900
+أساسي يسبق المواد والمساقات التخصصية الأخرى، يعني من
+
+22
+00:01:35,900 --> 00:01:39,040
+خلال هذا الـ course احنا بنتعلم كيف نتعامل مع
+
+23
+00:01:39,040 --> 00:01:42,740
+المختبر، كيف نتعامل مع عينات المختبر، كيف نحصل على
+
+24
+00:01:42,740 --> 00:01:47,700
+عينة من المريض، كيف أتعامل مع المريض ومع زملائي ومع
+
+25
+00:01:47,700 --> 00:01:51,540
+الأدوات والأجهزة اللي موجودة داخل المختبر بحيث
+
+26
+00:01:51,540 --> 00:01:57,080
+أن أنتم يعني تتأهلوا للمساقات الأخرى الأكثر
+
+27
+00:01:57,080 --> 00:02:01,100
+تخصصية يكون عندكم background يكون عندكم خلفية، كيف
+
+28
+00:02:01,100 --> 00:02:05,380
+أنتم يعني تجرّبوا فحوصات أكثر تخصصا وأكثر تقدما،
+
+29
+00:02:05,380 --> 00:02:10,240
+تمام؟ فهنا الحاجة للأساس، هنا يعني الأساسية
+
+30
+00:02:10,240 --> 00:02:14,160
+والتأسيس للتخصص،
+
+31
+00:02:14,160 --> 00:02:18,740
+إذن هو مساق لازم يسبق أي مادة تخصص آخر أو يعني هو
+
+32
+00:02:18,740 --> 00:02:24,400
+مساق لازم يسبق أي تخصص أو sorry يعني هو مساق لازم
+
+33
+00:02:24,400 --> 00:02:31,590
+يسبق أي مساق تخصص آخر لحتى يكون عندكم الـ...الأساس
+
+34
+00:02:31,590 --> 00:02:37,370
+اللي تتمكنوا من بعده تبنوا عليه أي معرفة أكثر
+
+35
+00:02:37,370 --> 00:02:44,350
+عمقا أو أكثر اتساعا، طبعا؟ في النهاية ال...يعني
+
+36
+00:02:44,350 --> 00:02:47,350
+راح أكون معكم أنا أستاذة نور أبو تيم، أستاذة نور
+
+37
+00:02:47,350 --> 00:02:51,520
+أبو تيم بكالوريوس علوم طبية مخبرية من الجامعة
+
+38
+00:02:51,520 --> 00:02:55,280
+الإسلامية، و ماستر علم دم وبنك دم من جامعة العلوم
+
+39
+00:02:55,280 --> 00:02:57,980
+والتكنولوجيا الأردنية، و...
+
+40
+00:03:01,020 --> 00:03:05,800
+أتحدث معكم، المواضيع اللي راح نتناولها، بداية تقديم
+
+41
+00:03:05,800 --> 00:03:09,280
+اللي إحنا عم نحكي فيه هلق هو Introduction to the
+
+42
+00:03:09,280 --> 00:03:12,380
+course، بعد هيك راح نحكي عن شيء اسمه medical
+
+43
+00:03:12,380 --> 00:03:16,340
+laboratories، راح نعرف المختبرات الطبية، أنواع
+
+44
+00:03:16,340 --> 00:03:22,530
+المختبرات الطبية ورسالة الأخصائي في المختبرات الطبية
+
+45
+00:03:22,530 --> 00:03:28,330
+كيف أنه هو يعني، لأ كيف يجب أن يتعامل داخل المختبر
+
+46
+00:03:28,330 --> 00:03:33,150
+وكل ما يتعلق بالمختبرات الطبية، أنواع أو بعض
+
+47
+00:03:33,150 --> 00:03:36,840
+المصطلحات والتعريفات المتعلقة فيه، راح ناخذها في
+
+48
+00:03:36,840 --> 00:03:41,880
+هذا الجزء، في الموضوع الثالث راح نحكي عن الـ
+
+49
+00:03:41,880 --> 00:03:47,500
+general laboratory safety، طبعا حالنا حال أي مادة
+
+50
+00:03:47,500 --> 00:03:52,860
+عملية لازم نبلش بالحكي عن قوانين السلامة والأمان
+
+51
+00:03:52,860 --> 00:03:57,310
+اللي موجودة أو اللي يجب أن تُطبّق داخل المختبرات، راح
+
+52
+00:03:57,310 --> 00:04:02,210
+نحكي عنها اليوم أو يعني الفصل هذا أو في المساق هذا
+
+53
+00:04:02,210 --> 00:04:05,710
+وراح نحكي طبعا كيف إحنا ممكن يعني أو كيف الـ
+
+54
+00:04:05,710 --> 00:04:08,750
+medical laboratory scientist، خاصة العلوم الطبية
+
+55
+00:04:08,750 --> 00:04:13,530
+المخبرية لازم عنده ثلاث أهداف يحققها، أول حاجة to
+
+56
+00:04:13,530 --> 00:04:19,540
+save himself، يعني أنه بده يحفظ على نفسه، يحمي
+
+57
+00:04:19,540 --> 00:04:23,660
+نفسه، to save patient، يعني أنه يحافظ على المريض ما
+
+58
+00:04:23,660 --> 00:04:26,780
+تنتقله عدوى من المختبر أو من العينات اللي موجودة
+
+59
+00:04:26,780 --> 00:04:29,860
+داخل المختبر، إذن يجب أن تكون البيئة اللي بيشتغل
+
+60
+00:04:29,860 --> 00:04:34,460
+فيها نظيفة، و to save colleagues، اللي هو يعني يحافظ
+
+61
+00:04:34,460 --> 00:04:39,740
+على زملائه، هو لأ من خلال أنه ما يكون هو سبب
+
+62
+00:04:39,740 --> 00:04:43,790
+لانتقال العدوى من مثلا المرضى لزملائه اللي معكم هو
+
+63
+00:04:43,790 --> 00:04:48,270
+حلقة الوصل في انتقال العدوى، تمام؟ وراح نحكي أيضا
+
+64
+00:04:48,270 --> 00:04:56,080
+عن الـ infection control، اللي هي عبارة عن كيف أنا الـ
+
+65
+00:04:56,080 --> 00:04:59,660
+infection control، كيف أني أُحدّ من انتشار العدوى
+
+66
+00:04:59,660 --> 00:05:05,280
+وطبعا راح أحكي عنها بـ ISAB، هاي المجلة، لإنه راح
+
+67
+00:05:05,280 --> 00:05:09,900
+نربطها بكيف إني أكون جزء من الحد من انتشار
+
+68
+00:05:09,900 --> 00:05:13,580
+العدوى، ونربطها بـ COVID-19 pandemic، اللي هي جائحة
+
+69
+00:05:13,580 --> 00:05:18,510
+الـ corona virus اللي موجودة حاليا، لحتى نتعلم كيف أن
+
+70
+00:05:18,510 --> 00:05:24,310
+إحنا نوظف علمنا في الـ...الـ... الظروف والمواقف
+
+71
+00:05:24,310 --> 00:05:28,410
+اللي بنمر فيها، biosafety، الأمانة والسلامة اللي
+
+72
+00:05:28,410 --> 00:05:32,110
+موجودة داخل المختبر، كيف أنا أتخلص من العينات بسلامة
+
+73
+00:05:32,110 --> 00:05:38,730
+كيف...أن...أن المواد الكيميائية وال...والمحاليل
+
+74
+00:05:38,730 --> 00:05:43,190
+اللي أنا بَتعامل معها ما تكون خطر عليّ أو على
+
+75
+00:05:43,190 --> 00:05:49,410
+حياتي، في محاليل خانقة، في محاليل حارقة، في محاليل
+
+76
+00:05:49,410 --> 00:05:56,390
+ممكن أنها تؤدي إلى...يعني تسربها يؤدي إلى إشعاع،
+
+77
+00:05:56,390 --> 00:06:03,360
+في محاليل ممكن تؤدي إلى حرائق، لا سمح الله، في أيضا
+
+78
+00:06:03,360 --> 00:06:06,960
+عينات لازم طبعا ممكن تكون infectious، كيف أنا ممكن
+
+79
+00:06:06,960 --> 00:06:11,360
+أتلفها، هي كل الشغلات اللي بتتعلق بـ إني أنا هكذا
+
+80
+00:06:11,360 --> 00:06:14,140
+أحمي حالي من الأشياء اللي موجودة داخل المختبر سواء
+
+81
+00:06:14,140 --> 00:06:18,150
+كانت أدوات أو أجهزة أو محاليل أو عينات، راح يندرج
+
+82
+00:06:18,150 --> 00:06:22,310
+تحت مصطلح الـ biosafety lab equipments، راح نحكي
+
+83
+00:06:22,310 --> 00:06:27,550
+أيضا عن الأدوات اللي موجودة داخل المختبر وكيف أنا
+
+84
+00:06:27,550 --> 00:06:32,650
+بستخدمها وأشوف فائدتها، وراح نتطرق أيضا لـ...
+
+85
+00:06:32,650 --> 00:06:37,310
+أحيانا للمقارنة ما بين أنواع الأدوات اللي موجودة و
+
+86
+00:06:37,310 --> 00:06:42,250
+دقتها، Okay، طيب بعد ذلك راح نحكي عن المحاليل اللي
+
+87
+00:06:42,250 --> 00:06:46,050
+بتستخدم داخل المختبر، أنواع هذه المحاليل وكيف
+
+88
+00:06:46,050 --> 00:06:49,610
+تتحضر وكيف أنا أعمل لها تخفيف لحتى أستخدمها وتكون
+
+89
+00:06:49,610 --> 00:06:53,570
+جاهزة للاستخدام، وطبعا يعني إحنا بنعرف أن كل
+
+90
+00:06:54,750 --> 00:06:57,750
+ابحاثنا هي عبارة عن تفاعلات كيميائية، الفحوصات
+
+91
+00:06:57,750 --> 00:07:00,050
+اللي أنا بدي أجريها على عينة المريض هي عبارة عن
+
+92
+00:07:00,050 --> 00:07:03,470
+تفاعلات كيميائية، محتاجة العينة ومحتاجة المادة
+
+93
+00:07:03,470 --> 00:07:06,810
+المتفاعلة معها، المادة المتفاعلة معها هذه عبارة عن
+
+94
+00:07:06,810 --> 00:07:11,830
+محاليل، كيف تستخدم، كيف تتحضر، كيف بنحصل عليها، هذه
+
+95
+00:07:11,830 --> 00:07:17,550
+كلها راح تكون موجودة في المعلومات في تحت عنوان
+
+96
+00:07:17,550 --> 00:07:20,390
+solution used in medical laboratory، كل المحاليل
+
+97
+00:07:20,390 --> 00:07:23,250
+اللي تستخدم في الاختبارات الطبية راح نحكي عنها
+
+98
+00:07:24,320 --> 00:07:28,280
+أنواعها كمان، راح نحكي عن حاجة اسمها automatic by-
+
+99
+00:07:28,280 --> 00:07:31,840
+pipette use and calibration، شو يعني automatic by-pipette؟
+
+100
+00:07:31,840 --> 00:07:35,480
+إذا أخذتو أنتم معاهم الـ...الـ... الكيمياء
+
+101
+00:07:35,480 --> 00:07:40,860
+الأساسية الـ... أكيد مر معكم اللي هي، لفظ الصحيح
+
+102
+00:07:40,860 --> 00:07:43,360
+أو الـ by-pipette اللي كنتوا تستخدموها to transfer
+
+103
+00:07:43,360 --> 00:07:48,240
+volumes، يعني لتنقلوا حجوم، طبعا إتعلمتو كيف الـ
+
+104
+00:07:48,240 --> 00:07:52,860
+use لها، كيف استخدامها، وأيضا كيف أني أعمل لها
+
+105
+00:07:52,860 --> 00:07:58,480
+calibration، كيف يعني، طبعا استخدامها طبعا إحنا في
+
+106
+00:07:58,480 --> 00:08:02,920
+المعامل هنا بتـ... بنحكي عن نوع من الـ pipette اللي هو
+
+107
+00:08:02,920 --> 00:08:05,900
+متقدم استخدامه، نوع عام اللي هو automatic pipette
+
+108
+00:08:06,330 --> 00:08:09,130
+طبعا أنا ليش بستخدم الـ pipette الـ automatic؟ أول حاجة
+
+109
+00:08:09,130 --> 00:08:13,250
+لأنه بدي درجة عالية من الـ accuracy ودرجة عالية
+
+110
+00:08:13,250 --> 00:08:17,330
+من الـ precision، الصحة والدقة، ليش؟ أنا معنية أن
+
+111
+00:08:17,330 --> 00:08:21,230
+أنا أستخدم pipette دقيقة، pipette accurate and precise
+
+112
+00:08:21,230 --> 00:08:27,480
+لحتى أنقل حجم بدقة ومن ثم أجري تفاعل دقيق، أحصل على
+
+113
+00:08:27,480 --> 00:08:31,340
+نتائج صحيحة، وبالتالي أنا أعبر عن صحة المريض
+
+114
+00:08:31,340 --> 00:08:36,180
+أو أعبر عن حالته بشكل صحيح، وهكذا أنا بساهم أن أنا
+
+115
+00:08:36,180 --> 00:08:41,540
+بإنقاذ روح هذا المريض، أيضا نحتاج لـ calibration
+
+116
+00:08:41,540 --> 00:08:46,160
+يعني أني كيف أعيد تهيئة أو ضبط هاي الـ pipette في
+
+117
+00:08:46,160 --> 00:08:50,280
+حال لو كان اكتشفت أن فيها خلل أو error، كيف أنا بدي
+
+118
+00:08:50,280 --> 00:08:55,690
+أضبطها، راح نحكي أيضا عن الـ blood، blood sample
+
+119
+00:08:55,690 --> 00:08:59,790
+collection and processing، كيف أنا أسحب عينة دم من
+
+120
+00:08:59,790 --> 00:09:04,850
+مريض، سواء كان هذا المريض adult، سواء يعني شخص كبير
+
+121
+00:09:04,850 --> 00:09:12,510
+بالعمر بالغ أو شخص newborn، طفل حديث الولادة، من وين
+
+122
+00:09:12,510 --> 00:09:15,870
+أنا باخذ العينة سواء كانت من الوريد أو من الـ skin
+
+123
+00:09:15,870 --> 00:09:19,570
+كيف طريقة أخذ العينة، كل هذا راح نتعلمه في الـ blood
+
+124
+00:09:19,570 --> 00:09:24,730
+sample collection، وأيضا راح نتعرف كيف أنا بتعامل مع
+
+125
+00:09:24,730 --> 00:09:28,670
+عينة الدم هذه، يعني في بعض الفحوصات بتحتاج أنه أنا
+
+126
+00:09:28,670 --> 00:09:33,770
+بدي أفصل هذه العينة لحتى أحصل على مثلا جزء من
+
+127
+00:09:33,770 --> 00:09:38,090
+مكونات المادة، يعني أنا لو بدي فحص بيشتغل على
+
+128
+00:09:38,090 --> 00:09:42,530
+البلازما أو على خلايا، فأنا محتاجة لفحص بيشتغل على
+
+129
+00:09:42,530 --> 00:09:48,020
+جزء من مكونات العينة لحتى أكمل عليه، أحيانا في
+
+130
+00:09:48,020 --> 00:09:50,920
+فحوصات بتحتاج الـ whole blood، يعني الدم ككل، أشتغل
+
+131
+00:09:50,920 --> 00:09:54,000
+عليه، فهنا محتاجة أني أنا مثلا فقط أني أنا أخزن
+
+132
+00:09:54,000 --> 00:09:58,320
+العينة أو أحتفظ فيها لحين استخدامها تحت ظروف معينة
+
+133
+00:09:58,320 --> 00:10:02,140
+أنه ما تخرب العينة، هذا كله راح نتعلمه في الـ blood
+
+134
+00:10:02,140 --> 00:10:04,720
+sample collection and processing، حكينا أن
+
+135
+00:10:04,720 --> 00:10:08,140
+المعلومات إن شاء الله راح تكون بالنسبة لـ...يعني
+
+136
+00:10:08,140 --> 00:10:11,020
+لما نـ... نفردها ونحكي عنها بالتفصيل إن شاء الله
+
+137
+00:10:11,020 --> 00:10:15,760
+ما راح يكون في شيء صعب أو يكون في شيء غامض، كل شيء
+
+138
+00:10:15,760 --> 00:10:20,160
+إن شاء الله راح نبسطه و نسهله لكم بحيث أن تستفيدوا
+
+139
+00:10:20,160 --> 00:10:24,300
+راح نحكي عن بعض الأجهزة، sorry، مش الأدوات، الأجهزة
+
+140
+00:10:24,300 --> 00:10:28,040
+اللي موجودة داخل المختبر، الـ instruments، راح نحكي
+
+141
+00:10:28,040 --> 00:10:32,580
+عن الـ microscope، أنواعه، طريقة استخدامه، طريقة
+
+142
+00:10:32,580 --> 00:10:38,760
+التعامل معه، مبدأ عمله، أجزاؤه، sorry، أجزاؤه
+
+143
+00:10:39,070 --> 00:10:44,530
+وكتطبيق على استخدام الـ microscope راح نأخذ طريقة
+
+144
+00:10:44,530 --> 00:10:48,090
+عدّ الـ white blood cells والـ RBCs، كيف أنا أعدّ خلايا
+
+145
+00:10:48,090 --> 00:10:51,290
+الدم البيضاء، عدد خلايا الدم البيضاء وخلايا الدم
+
+146
+00:10:51,290 --> 00:10:56,830
+الحمراء في عينة الدم، تمام؟ إذن بعد ما أحصل على عينة
+
+147
+00:10:56,830 --> 00:10:59,490
+الدم، بدي أتعلم كيف أعدّ الـ white blood cells وكيف أعدّ
+
+148
+00:10:59,490 --> 00:11:05,110
+الـ RBCs، بدي أتعلم كيف أجيب hematocrit، يعني كيف
+
+149
+00:11:05,110 --> 00:11:09,550
+أشتغل على الـ hematocrit، الـ hematocrit هو عبارة عن
+
+150
+00:11:09,550 --> 00:11:15,750
+النسبة أو المساحة أو الـ volume اللي بيحتلها الـ
+
+151
+00:11:15,750 --> 00:11:20,050
+RBCs بالنسبة للـ whole sample، يعني كمية يعني الحجم
+
+152
+00:11:20,050 --> 00:11:23,750
+اللي بيحتله الـ RBCs أو خلايا الدم الحمراء بالنسبة
+
+153
+00:11:23,750 --> 00:11:29,500
+للحجم اللي بيحتله العينة كاملة، فطبعا هذا أيضا يجري
+
+154
+00:11:29,500 --> 00:11:34,600
+على عينة الدم، بتطبيق جهاز اسمه centrifuge، راح نأخذ
+
+155
+00:11:34,600 --> 00:11:37,900
+أيضا فحص اللي هو الـ hemoglobin concentration
+
+156
+00:11:37,900 --> 00:11:42,380
+determination، يعني أنا بدي أحسب تركيز بروتين الـ
+
+157
+00:11:42,380 --> 00:11:46,230
+hemoglobin اللي هو يعني بالعامية أو بالعربية بنحكي
+
+158
+00:11:46,230 --> 00:11:50,750
+عنه قوة الدم، بدي أقيس قوة الدم، فبدي أقيس تركيز الـ
+
+159
+00:11:50,750 --> 00:11:53,710
+hemoglobin اللي هو عبارة عن الـ oxygen carrying
+
+160
+00:11:53,710 --> 00:11:57,290
+protein، اللي هو الـ protein اللي ناقل الـ oxygen
+
+161
+00:11:57,290 --> 00:12:02,650
+داخل خلايا الدم الحمراء، وبهذا تقريبا تقريبا أنا
+
+162
+00:12:02,650 --> 00:12:09,290
+بكون طبعا مع محاضرة الـ blood indices، يعني محاضرة
+
+163
+00:12:09,290 --> 00:12:16,800
+نظرية راح نحكي عنها، هيك تقريبا أنا بكون عملت حاجة
+
+164
+00:12:16,800 --> 00:12:22,820
+اسمها CBC، بس أخلص هاي المواضيع وتشتغلوها بتكونوا
+
+165
+00:12:22,820 --> 00:12:28,640
+اشتغلتوا CBC ولكن يدويا، شو يعني CBC؟ يعني complete
+
+166
+00:12:28,640 --> 00:12:35,460
+blood count، يعني عدّ كامل أو فحص شامل للدم، تمام؟ هذا
+
+167
+00:12:35,460 --> 00:12:40,340
+الفحص أنتم في معامل علم الدم والدم التشخيصي يعني
+
+168
+00:12:40,340 --> 00:12:45,020
+في المختبرات التخصصية القادمة يعني في سنة ثالثة و
+
+169
+00:12:45,020 --> 00:12:50,300
+رابعة راح تشتغلوا باستخدام أجهزة، هذا الفحص يعني
+
+170
+00:12:50,300 --> 00:12:54,960
+فقط بتدخلوا عينة على الجهاز والجهاز يطلع لكم كل شيء
+
+171
+00:12:54,960 --> 00:13:02,240
+مقروء جاهز، لكن هنا بتشتغلوا بأيديكم، وهذا هو ميزة
+
+172
+00:13:02,240 -->
+
+223
+00:16:49,840 --> 00:16:54,360
+أه، ييجي في بالي المرض الفلاني، فطبعاً يعني
+
+224
+00:16:54,360 --> 00:16:58,820
+مش... لازم تمروا بمراحل اللي هي مراحل تعلم
+
+225
+00:16:58,820 --> 00:17:05,100
+تحضير المسحة، مع مراحل تعلم صبغ المسحة، و أيضاً رؤية
+
+226
+00:17:05,100 --> 00:17:09,360
+الخلايا، على الأقل اللي تشوفه normal عشان لما بقعد
+
+227
+00:17:09,360 --> 00:17:12,780
+هيك في المساقات الأخرى تشوفوا الـ abnormal تكونوا
+
+228
+00:17:12,780 --> 00:17:16,580
+قادريين تتميزوا ما بين الطبيعي و الغير طبيعي، هذا
+
+229
+00:17:16,580 --> 00:17:21,700
+بالنسبة للمواضيع اللي إحنا تناولناها خلال ال...
+
+230
+00:17:22,430 --> 00:17:30,070
+المساق هنا فعلياً زي ما أنتم شايفين، فيه مواضيع هي
+
+231
+00:17:30,070 --> 00:17:36,010
+مواضيع نظرية بحتة، و فيه مواضيع لأ، هي نظرية و عملية
+
+232
+00:17:36,010 --> 00:17:40,050
+عشان هيك بتلاحظوا أن المساق لما أنتم جئتم سجلتموه
+
+233
+00:17:40,050 --> 00:17:45,210
+عبارة عن مقدمة في التحليل الطبي، مناقشة يعني بينا
+
+234
+00:17:45,210 --> 00:17:49,110
+... إحنا راح نحكي في تحت هذا الـ band كل المواضيع
+
+235
+00:17:49,110 --> 00:17:54,280
+اللي هي نظرية بحتة، مش فيها عملي خالص، بيكون راح
+
+236
+00:17:54,280 --> 00:17:57,840
+نحكي تحت band المقدمة في التحليل اللي هو العملي
+
+237
+00:17:57,840 --> 00:18:03,580
+المواضيع العملية، هلأ إحنا راح نغطي جزء النظري
+
+238
+00:18:03,580 --> 00:18:08,940
+النظري و العملي، و راح إن شاء الله يعني زي ما
+
+239
+00:18:08,940 --> 00:18:12,140
+صار في الفصول... في الفصل السابق إحنا يعني أعطينا
+
+240
+00:18:12,140 --> 00:18:16,600
+المُحاضرات، بعد هيك أعطينا شهر استدراكي تعويضي، أن كل
+
+241
+00:18:16,600 --> 00:18:22,550
+المعامل عوضناها، يعني جِبنا الطالبات على ال... معامل
+
+242
+00:18:22,550 --> 00:18:26,990
+و وزّعناهم جروبات، بحيث ما يكون فيه تكدس، يكون فيه
+
+243
+00:18:26,990 --> 00:18:32,250
+تباعد، سبل وقاية، و خليناهم يشتغلوا الجزئية العملية
+
+244
+00:18:32,250 --> 00:18:38,550
+اللي موجودة معاهم، فيعني أنا أتوقع و يعني القسم
+
+245
+00:18:38,550 --> 00:18:42,310
+أكيد يعني راح إن شاء الله يطبّق هذا ال... الأشياء
+
+246
+00:18:42,780 --> 00:18:48,880
+فالجزئية العملية راح ن... ن... نؤجلها لحين عودتنا
+
+247
+00:18:48,880 --> 00:18:54,080
+إن شاء الله، أو لحين وضع خطة للتعويض العملي، تمام؟
+
+248
+00:18:54,080 --> 00:18:58,280
+ولكن لحتى ما يسرقنا الوقت، إحنا راح نظل مستمرين على
+
+249
+00:18:58,280 --> 00:19:01,920
+الأقل، لنغطي الجزئية النظرية من خلال المحاضرات الـ
+
+250
+00:19:01,920 --> 00:19:07,230
+online، زي ما أنتم عارفين، طبيعة المساق هو ساعة
+
+251
+00:19:07,230 --> 00:19:11,590
+مناقشة، و ثلاث ساعات lab، يعني كان مطلوب منكم لو
+
+252
+00:19:11,590 --> 00:19:14,350
+أنتم بتيجوا على المختبرات، و بتداوموا، كان مطلوب
+
+253
+00:19:14,350 --> 00:19:18,270
+منكم، و أربع ساعات تلتزموا بال... بتعطوها من وقتكم
+
+254
+00:19:18,270 --> 00:19:23,730
+حق للمادة، فأنا هلأ بدي أطلب منكم أنه تتحضروا لي يعني
+
+255
+00:19:23,730 --> 00:19:29,290
+تتفقوا على ساعة أنزل لكم فيها، أو يعني توقيت أنزل لكم
+
+256
+00:19:29,290 --> 00:19:35,240
+فيه المحاضرة النظرية، اللي هي المناقشة، و المحاضرة
+
+257
+00:19:35,240 --> 00:19:41,180
+العملية، راح نشرح الجزء النظري منها، و نسيب العملي
+
+258
+00:19:41,180 --> 00:19:47,840
+لحين التعويض، و جزء ساعة لـ... أن نكون موجودين
+
+259
+00:19:47,840 --> 00:19:53,820
+كلنا في نفس ذات اللحظة، على موقع الـ BB اللي هو يعني
+
+260
+00:19:53,820 --> 00:19:59,260
+موقع الـ online، تبع الـ model، أو ال... الـ zoom، أو
+
+261
+00:19:59,260 --> 00:20:03,660
+موقع class، google meeting، أي موقع أنتم بتختاروه
+
+262
+00:20:03,660 --> 00:20:07,660
+بيكون فيه إجماع عليه، أنا ما عندي مشكلة، و بحيث أنّه
+
+263
+00:20:07,660 --> 00:20:11,660
+نكون كلنا موجودين، لو ما فهمتوا شيء أثناء شرح
+
+264
+00:20:11,660 --> 00:20:15,640
+المحاضرة، أو تسجيل المحاضرة، بنفتح المحاضرة، بنرجع لـ
+
+265
+00:20:15,640 --> 00:20:21,660
+الـ slides، بنرجع عليها، بنفهمها، إذا في عندكم أي
+
+266
+00:20:21,660 --> 00:20:25,980
+استفسار أو أي سؤال، بتسألوني... يعني بس تسألوني و
+
+267
+00:20:25,980 --> 00:20:30,340
+بجاوبكم عليه، ممكن يكون في نقاش، و النقاش طبعاً معروف
+
+268
+00:20:30,340 --> 00:20:37,480
+أنّه هو يثري المعلومات، ف... و بعمق الفهم، فيعني هدول
+
+269
+00:20:37,480 --> 00:20:41,300
+الثلاث ساعات، لغير عنهم لازم أنّه... يعني يكون في عندك
+
+270
+00:20:41,660 --> 00:20:46,940
+ساعة تسمع فيها تسجيل المحاضرة النظرية... المحاضرة
+
+271
+00:20:46,940 --> 00:20:51,720
+العملية، ساعة أيضاً، و ساعة النقاش. هلأ نيجي لتوزيع
+
+272
+00:20:51,720 --> 00:20:56,980
+الدرجات، 30% من المادة راح يكون على الـ quizzes و الـ
+
+273
+00:20:56,980 --> 00:21:01,800
+midterm exam، يعني راح أعطيكم مثلاً، بعد ما نخلص ثلاث
+
+274
+00:21:01,800 --> 00:21:05,840
+معامل مثلاً، أو أربعة، رح أُحدد بالاتفاق معاكم على
+
+275
+00:21:05,840 --> 00:21:11,700
+موعد و توقيت لـ quiz، بنزل لكم الأسئلة على الـ model
+
+276
+00:21:11,700 --> 00:21:16,440
+بتدخلوا في موعد الـ quiz، بتقدموا الـ quiz، و بتطلعوا
+
+277
+00:21:16,440 --> 00:21:19,620
+ففي النهاية أنا بحسب لكم على مجموع الـ quiz ذات و
+
+278
+00:21:19,620 --> 00:21:23,540
+الامتحان النصفي، ثلاثين درجة، أربعين درجة على الـ
+
+279
+00:21:23,540 --> 00:21:27,460
+final exam، و ثلاثين على... على الـ lab work و الـ
+
+280
+00:21:27,460 --> 00:21:30,880
+experiment، هذا في حالة لما نرجع على المختبرات
+
+281
+00:21:30,880 --> 00:21:35,240
+و نطبق التجارب، راح يكون في ثلاثين درجة على التزامكم
+
+282
+00:21:35,240 --> 00:21:40,000
+بالدوام أثناء التعويض، التزامكم بالتجربة... بالتجربة
+
+283
+00:21:40,000 --> 00:21:45,420
+ب... فهمكم للتجربة، شغلكم، و حرصكم على أنكم أنتم تشتغلوا و
+
+284
+00:21:45,420 --> 00:21:49,180
+تسلموا نتائج، ما... مش راح يكون فيه تقييم على صحة
+
+285
+00:21:49,180 --> 00:21:52,330
+النتائج، لأن أنتم جايين تتعلموا، و أنتم بتدخلوا
+
+286
+00:21:52,330 --> 00:21:56,930
+مختبرات طبية لأول مرة، فمش رح أجي أحقق... أقيمكم أن
+
+287
+00:21:56,930 --> 00:22:00,610
+والله نتيجيتكم اللي سلمتولي إياها صح أو خطأ، و أنتم جايين
+
+288
+00:22:00,610 --> 00:22:04,910
+تتعلموا، لأ، رح أسأل... رح... رح أقيمكم في حال أنه
+
+289
+00:22:04,910 --> 00:22:09,570
+يعني الـ attitude تبعكم، يعني أن أنتم هل كنتم على
+
+290
+00:22:09,570 --> 00:22:14,670
+استعداد أنكم تتعلموا، ولا كنتم care less، سلمتوا لي نتائج
+
+291
+00:22:14,670 --> 00:22:18,490
+ولا ما سلمتوا، اشتغلتوا ولا ما اشتغلتوا، طبقتوا التعليمات
+
+292
+00:22:18,490 --> 00:22:22,760
+اللي أنا حكيتها، بحدّ أوفيرها ولا لأ، ال... ال... مدى
+
+293
+00:22:22,760 --> 00:22:29,420
+الالتزام، و مدى الاهتمام بالحضور، و بالمدى الجدية
+
+294
+00:22:29,420 --> 00:22:33,960
+داخل المختبر، هدول اللي عليهم ثلاثين علامة، مش صحة
+
+295
+00:22:33,960 --> 00:22:37,940
+النتائج نفسها، لأن في مختبرات ثانية، المواد التخصص
+
+296
+00:22:37,940 --> 00:22:42,740
+الثانية، رح تبدأ تتحاسبوا على صحة النتائج، لكن أنتم هنا
+
+297
+00:22:42,740 --> 00:22:46,540
+اليوم جايين تتعلموا، جايين تستخدموا أدوات، و تعملوا
+
+298
+00:22:46,540 --> 00:22:51,480
+فحوصات، و تستخدموا أجهزة لأول مرة تستخدمونها، ف أنا مش
+
+299
+00:22:51,480 --> 00:22:56,920
+راح أحاسبكم على خطأ في... في كون أنكم أنتم جايين لأول
+
+300
+00:22:56,920 --> 00:23:01,520
+مرة، بتدخلوا مختبر طبي، و بتمارسوا ال... الفحوصات، و
+
+301
+00:23:01,520 --> 00:23:06,830
+النشاطات اللي موجودة داخل المختبر، في عندي جزء
+
+302
+00:23:06,830 --> 00:23:12,110
+باللغة العربية، على شرح المادة، و توزيع الدرجات،
+
+303
+00:23:12,110 --> 00:23:16,550
+عندكم إيميلي، إذا حابين ترسلوا لي عليه، في أيضاً حساب
+
+304
+00:23:16,550 --> 00:23:19,450
+الـ facebook، و حساب الـ instagram، و الـ whatsapp، على
+
+305
+00:23:19,450 --> 00:23:22,550
+حسابكم، يعني كل شيء حابين تتواصلوا معي فيه
+
+306
+00:23:22,550 --> 00:23:28,590
+تواصلوا معي، أنا جاهزة لأقدم لكم يعني أي جواب
+
+307
+00:23:28,590 --> 00:23:34,260
+لأي استفسار، أو أي سؤال عندكم، هلأ أنا ممكن ما أكون
+
+308
+00:23:34,260 --> 00:23:37,620
+ذكرت كل المعلومات اللي أنتم حابين تعرفوها، ممكن
+
+309
+00:23:37,620 --> 00:23:44,220
+أكون خلفت عن جزئية معينة، فلهيك بحب أنّه إذا كان في
+
+310
+00:23:44,220 --> 00:23:48,260
+عندكم استفسار، حابين تعرفوا أي شيء بيتعلق بـ...
+
+311
+00:23:48,400 --> 00:23:54,560
+التقديم للمادة، أو محتوى المادة، أو أي شيء بيتعلق
+
+312
+00:23:54,560 --> 00:23:59,880
+بالمادة ككل، عادي اسألوني، و برجع بجاوبكم، سواء كان
+
+313
+00:23:59,880 --> 00:24:02,700
+على group الـ whatsapp، أو على أي موقع من مواقع الـ
+
+314
+00:24:02,700 --> 00:24:07,160
+social media اللي أنتم بتعرفوا حسابي عليهم، هيك
+
+315
+00:24:07,160 --> 00:24:14,560
+أنا بكون خلصت، يعطيكم العافية، طبعاً موعد شوف يعني، ثلاث
+
+316
+00:24:14,560 --> 00:24:17,560
+مواعيد، أنا بدي إياها تتحدد بالاتفاق ما بينكم، بلاش
+
+317
+00:24:17,560 --> 00:24:22,660
+أفرضهم عليكم، يعني، بش ما أكون شريرة من أولها، طبعاً
+
+318
+00:24:22,660 --> 00:24:30,130
+أه، بالنسبة للجزئية العملية، زي ما حكيت لكم، أنّه إحنا
+
+319
+00:24:30,130 --> 00:24:34,930
+هنعوّض، ولكن في بعض ال... النشاطات، أو بعض التجارب
+
+320
+00:24:34,930 --> 00:24:39,910
+راح نشوفها مع بعض، مثلاً من خلال فيديوهات، أجيب لكم
+
+321
+00:24:39,910 --> 00:24:43,190
+فيديو نحضّره أنا و أنتم سوا، على... في ال... في الـ
+
+322
+00:24:43,190 --> 00:24:49,610
+discussion، لحتى تفهموا أكثر أنا عن شو بحكي، في مثلاً
+
+323
+00:24:49,610 --> 00:24:56,470
+ممكن يعني أجيب حدا من عائلتي، يتبرّع أنّه مثلاً أسحب له
+
+324
+00:24:56,470 --> 00:25:01,370
+عينة دم، قدامكم، تشوفوا كيف بتتم عملية السحب، يعني
+
+325
+00:25:01,370 --> 00:25:04,950
+نحاول على الأقل يكون في عندكم تصوّر، لأن إحنا إيش
+
+326
+00:25:04,950 --> 00:25:09,550
+بنشتغل، حتى يعني لما تيجوا تمارسوا بإيديكم، يكون سهل
+
+327
+00:25:09,550 --> 00:25:14,750
+عليكم هذا الشيء، ف إن شاء الله أنا راح أحاول أنّه أتبع
+
+328
+00:25:14,750 --> 00:25:18,090
+معاكم هذا الأسلوب، إن شاء الله ما رح يكون في صعوبة
+
+329
+00:25:18,090 --> 00:25:23,370
+ما بدي أنّه مثلاً تخافوا، أو ترتبكوا، أو شيء، كل بداية
+
+330
+00:25:23,370 --> 00:25:28,270
+بتكون فيها رهبة، أي نعم، ولكن يعني، ما خاب
+
+331
+00:25:28,270 --> 00:25:32,150
+من استعان بالله، و حاولوا أنّه هيك تكونوا relaxed،
+
+332
+00:25:32,150 --> 00:25:37,370
+تكونوا بكل أريحية، يعني، على استعداد أنكم تتواصلوا
+
+333
+00:25:37,370 --> 00:25:44,560
+معي، لحتى يعني أوضح لكم أي شيء مبهم بالنسبة لكم، و
+
+334
+00:25:44,560 --> 00:25:50,980
+بس هيك، يعطيكم العافية، و شكراً كتير لاستماعكم، موفقين
+
+335
+00:25:50,980 --> 00:25:51,600
+بإذن الله
diff --git a/PL9fwy3NUQKwY9jl8xw-bWkVZkWcGGyspd/xD3hYPD-Pa0_postprocess.srt b/PL9fwy3NUQKwY9jl8xw-bWkVZkWcGGyspd/xD3hYPD-Pa0_postprocess.srt
new file mode 100644
index 0000000000000000000000000000000000000000..1e871ab7cd2cbaaaa8ab30dcc03c32b648e26440
--- /dev/null
+++ b/PL9fwy3NUQKwY9jl8xw-bWkVZkWcGGyspd/xD3hYPD-Pa0_postprocess.srt
@@ -0,0 +1,1340 @@
+1
+00:00:03,740 --> 00:00:07,540
+السلام عليكم ورحمة الله وبركاته أهلا وسهلا بيكم
+
+2
+00:00:07,540 --> 00:00:10,260
+عزيزتي طالبات نفس التواتر التاني من قسم العلم
+
+3
+00:00:10,260 --> 00:00:15,360
+الطبية المخبرية أنا عم بسجل هذه المحاضرة للمرة
+
+4
+00:00:15,360 --> 00:00:20,440
+التانية بعد ما جزء من الطالبات أو عدد من الطالبات
+
+5
+00:00:20,440 --> 00:00:25,520
+اشتكى أنه في نص ال record أو في نص التسجيل بيروح
+
+6
+00:00:25,520 --> 00:00:29,420
+.. بتروح الصورة فطلبت من البعض أنه يتبعوا مع ..
+
+7
+00:00:29,420 --> 00:00:32,740
+يعني يفتحوا ملف ال word زي ما أنا فاتحته هكو
+
+8
+00:00:32,740 --> 00:00:36,440
+يتبعوا معايا على كلامي بس يبدو أنه لسه كان المشكلة
+
+9
+00:00:36,440 --> 00:00:43,250
+قائمة و مش .. يعني كبداية لسه مش عارفين ف ..حابة
+
+10
+00:00:43,250 --> 00:00:46,110
+يعني خلاص بالآخر قررت أنه بدي أسجل هذه المحاضرة
+
+11
+00:00:46,110 --> 00:00:50,470
+مرة تانية أول إشي احنا أهلنا وسهلنا فيكم و حكنا
+
+12
+00:00:50,470 --> 00:00:54,650
+لكم أهلا وسهلا فيكم في القسم و أنه يعني كان بودنا
+
+13
+00:00:54,650 --> 00:00:59,350
+أنه نشوفكم face to face و نلتقي فيكم و نعرفكم عن
+
+14
+00:00:59,350 --> 00:01:03,410
+القسم بس يعني هاي الأزمة حالت دون ذلك و ب .. و لكن
+
+15
+00:01:03,410 --> 00:01:06,570
+ان شاء الله يعني كلنا أمل أنه هيتعالي الأزمة تعزي
+
+16
+00:01:06,570 --> 00:01:11,070
+على خير بأقل الخسائر و نرجع لمختبراتنا و نرجع لل
+
+17
+00:01:11,070 --> 00:01:15,230
+..القسم نرجع نشوفكم و نقرب ان شاء الله و لعله خير
+
+18
+00:01:15,230 --> 00:01:19,270
+يعني نأمل ده عليه راح نكون مع بعض ان شاء الله
+
+19
+00:01:19,270 --> 00:01:25,030
+بمثاق مقدمة في العلوم الطبية المخبرية او مقدمة في
+
+20
+00:01:25,030 --> 00:01:30,130
+التحليل الطبية اللي هي عبارة عن مثاق تأسيسي و
+
+21
+00:01:30,130 --> 00:01:35,900
+أساسي يسبق المواد و المثاقات التخصص الأخرىيعني من
+
+22
+00:01:35,900 --> 00:01:39,040
+خلال هذا ال course احنا بنتعلم كيف نتعامل مع
+
+23
+00:01:39,040 --> 00:01:42,740
+المختبر، كيف نتعامل مع عينات المختبر، كيف نحصل على
+
+24
+00:01:42,740 --> 00:01:47,700
+عينة من المريض، كيف أتعامل مع المريض و مع زملاء مع
+
+25
+00:01:47,700 --> 00:01:51,540
+الأدوات و الأجهزة اللي موجودة داخل المختبر بحيث
+
+26
+00:01:51,540 --> 00:01:57,080
+انه انا يعني تتأهلوا انه بالمثلقات الأخرى الأكثر
+
+27
+00:01:57,080 --> 00:02:01,100
+تخصيصية تكون عندكم background يكون عندكم خلفيةكيف
+
+28
+00:02:01,100 --> 00:02:05,380
+أنتوا يعني تجرب حوصات أكتر تخصصا و أكتر تقدما،
+
+29
+00:02:05,380 --> 00:02:10,240
+تمام؟ فهنا الحاجة للأساس، هنا يعني الأساسية
+
+30
+00:02:10,240 --> 00:02:14,160
+والتأسيس للتخصص،
+
+31
+00:02:14,160 --> 00:02:18,740
+إذا هو تخصص لازم يسبق أي مادة تخصص أخر أو يعني هو
+
+32
+00:02:18,740 --> 00:02:24,400
+مساق لازم يسبق أي تخصص أو sorry يعني هو مساق لازم
+
+33
+00:02:24,400 --> 00:02:31,590
+يسبق أي مساق تخصص آخرلحتى يكون عندكم ال .. الأساس
+
+34
+00:02:31,590 --> 00:02:37,370
+اللى تتمكنوا من بعد هيك تبنع عليه أي معرفة أكثر
+
+35
+00:02:37,370 --> 00:02:44,350
+عمقا أو أكثر اتساعا، طبعا؟ فى النهاية ال .. يعني
+
+36
+00:02:44,350 --> 00:02:47,350
+راح أكون معاكم أنا ناس نور أبوتيم، أستاذة نور
+
+37
+00:02:47,350 --> 00:02:51,520
+أبوتيم بكالوريوس علوم طبية مخبريةمن الجامعة
+
+38
+00:02:51,520 --> 00:02:55,280
+الإسلامية و master علم دم وبنك دم من جامعة العلم
+
+39
+00:02:55,280 --> 00:02:57,980
+والتكنولوجيا الأردنية و ..
+
+40
+00:03:01,020 --> 00:03:05,800
+أتحدث معاكم المواضيع اللي راح نتناولها بداية تقديم
+
+41
+00:03:05,800 --> 00:03:09,280
+اللي احنا عم نحكي فيه هلأ هو introduction to the
+
+42
+00:03:09,280 --> 00:03:12,380
+course بعد هيك راح نحكي عن اشي اسمه medical
+
+43
+00:03:12,380 --> 00:03:16,340
+laboratories راح نعرف المختبرات الطبية أنواع
+
+44
+00:03:16,340 --> 00:03:22,530
+المختبرات الطبية ورسالة الأخصائيالمختبرات الطبية
+
+45
+00:03:22,530 --> 00:03:28,330
+كيف أنه هو يعني لأ كيف يجب أن يتعامل داخل المختبر
+
+46
+00:03:28,330 --> 00:03:33,150
+و كل ما يتعلق بالمختبرات الطبية أنواع أو بعض
+
+47
+00:03:33,150 --> 00:03:36,840
+المصطلحات و التعريفات المتعلقة فيهارح ناخدها في
+
+48
+00:03:36,840 --> 00:03:41,880
+هذا الجزئية، في الموضوع التالت رح نحكي عن ال
+
+49
+00:03:41,880 --> 00:03:47,500
+general laboratory safety طبعا حالنا حال أي مادة
+
+50
+00:03:47,500 --> 00:03:52,860
+عملية لازم نبلش بالحكي عن قوانين السلامة والأمان
+
+51
+00:03:52,860 --> 00:03:57,310
+اللي موجودة أو اللي يجب أن تطبقداخل المختبرات راح
+
+52
+00:03:57,310 --> 00:04:02,210
+نحكي عنها اليوم او يعني الفصل هاد او في المساق هاد
+
+53
+00:04:02,210 --> 00:04:05,710
+و راح نحكي طبعا كيف احنا ممكن يعني او كيف ال
+
+54
+00:04:05,710 --> 00:04:08,750
+medical laboratory scientist خاصة العلوم الطبية
+
+55
+00:04:08,750 --> 00:04:13,530
+المخبرية لازم عنده تلت اهداف يحققها اول حاجة to
+
+56
+00:04:13,530 --> 00:04:19,540
+save himself يعني ان هو بدييحفظ على نفسه، يحمي
+
+57
+00:04:19,540 --> 00:04:23,660
+نفسه to save patient يعني أنه يحافظ على المريض ما
+
+58
+00:04:23,660 --> 00:04:26,780
+تنتقله عدوى من المختبر أو من العينات اللى موجودة
+
+59
+00:04:26,780 --> 00:04:29,860
+داخل المختبر إذا يجب أن تكون البيئة اللى بيشتغل
+
+60
+00:04:29,860 --> 00:04:34,460
+فيها نظيفة و to save colleagues اللى هو يعني يحافظ
+
+61
+00:04:34,460 --> 00:04:39,740
+على نسلة ميدز هو لأ من خلال أنه ما يكون هو سبب
+
+62
+00:04:39,740 --> 00:04:43,790
+لانتقال العدوى من مثلا المرضى لإلهماللي معاكم هو
+
+63
+00:04:43,790 --> 00:04:48,270
+حلقة الوصل في انتقال العدوى، تمام؟ و راح نحكي أيضا
+
+64
+00:04:48,270 --> 00:04:56,080
+عن ال infection control اللي هي عبارة عن كيف أناال
+
+65
+00:04:56,080 --> 00:04:59,660
+infection control كيف ان انا احد من انتشار العدوى
+
+66
+00:04:59,660 --> 00:05:05,280
+و طبعا راح احكي عنها ب ISAB هاي المجلة لإنه راح
+
+67
+00:05:05,280 --> 00:05:09,900
+نربطها بكيف انه انا اكون جزء من الحد من انتشار
+
+68
+00:05:09,900 --> 00:05:13,580
+العدوى و نربطها ب COVID-19 pandemic اللي هي جائحة
+
+69
+00:05:13,580 --> 00:05:18,510
+ال virus coronaاللي موجودة حاليا لحتى نتعلم كيف ان
+
+70
+00:05:18,510 --> 00:05:24,310
+احنا نوظف علمنا في ال .. ال .. الظروف والمواقف
+
+71
+00:05:24,310 --> 00:05:28,410
+اللي بنمر فيها biosafety الأمانة والسلام اللي
+
+72
+00:05:28,410 --> 00:05:32,110
+موجود داخل المختبر كيف انا اتخلص من العينات بسلامة
+
+73
+00:05:32,110 --> 00:05:38,730
+كيف .. ان .. ان المواد الكيميائية وال ..و المحاليل
+
+74
+00:05:38,730 --> 00:05:43,190
+اللي أنا بتعامل معها ما تكون خاطرة عليا أو على
+
+75
+00:05:43,190 --> 00:05:49,410
+حياتي، في محاليل خانقة، في محاليل حارقة، في محاليل
+
+76
+00:05:49,410 --> 00:05:56,390
+ممكن أنها تؤدي إلى .. يعني تسربها يؤدي إلى إشعاع،
+
+77
+00:05:56,390 --> 00:06:03,360
+في محاليل ممكن تؤدي إلى حرائق لصمح اللهفي أيضا
+
+78
+00:06:03,360 --> 00:06:06,960
+عينات لازم طبعا ممكن تكون infectious كيف انا ممكن
+
+79
+00:06:06,960 --> 00:06:11,360
+اتلفها، هي كل الشغلات اللي بتتعلق بيه انه انا كده
+
+80
+00:06:11,360 --> 00:06:14,140
+احمي حالي من الأشياء اللي موجودة داخل المختبر سواء
+
+81
+00:06:14,140 --> 00:06:18,150
+كانت أدوات أو أجهزة أو محاليل أو عيناتراح يندرج
+
+82
+00:06:18,150 --> 00:06:22,310
+تحت مصطلح ال biosafety lab equipments راح نحكي
+
+83
+00:06:22,310 --> 00:06:27,550
+أيضا عن الأدوات اللي موجودة داخل المختبر و كيف أنا
+
+84
+00:06:27,550 --> 00:06:32,650
+بستخدمها و أشوف فائدتها و راح نتطرق أيضا ل ..
+
+85
+00:06:32,650 --> 00:06:37,310
+أحيانا للمفادلة ما بين أنواع الأدوات اللي موجودة و
+
+86
+00:06:37,310 --> 00:06:42,250
+دقتهاOkay طيب بعد ذلك رح نحكي عن المحاليل اللي
+
+87
+00:06:42,250 --> 00:06:46,050
+بتستخدم داخل المختبر أنواع المحاليل هاي و كيف
+
+88
+00:06:46,050 --> 00:06:49,610
+تتحضر و كيف أنا أعملها تخفيفة لحتى أستخدمها و تكون
+
+89
+00:06:49,610 --> 00:06:53,570
+جاهزة للاستخدام و طبعا يعني احنا بنعرف انه كل
+
+90
+00:06:54,750 --> 00:06:57,750
+ابحثاتنا هي عبارة عن تفاعلات كيميائية الفحوصات
+
+91
+00:06:57,750 --> 00:07:00,050
+اللي أنا بدي أجريها على عينة المريض هي عبارة عن
+
+92
+00:07:00,050 --> 00:07:03,470
+تفاعلات كيميائية محتاجة العينة ومحتاجة المادة
+
+93
+00:07:03,470 --> 00:07:06,810
+المتفاعلة معها المادة المتفاعلة معها هذه عبارة عن
+
+94
+00:07:06,810 --> 00:07:11,830
+محاليل كيف تستخدم كيف تتحضر كيف بنحصل عليها هذه
+
+95
+00:07:11,830 --> 00:07:17,550
+كلها راح تكون موجودة في المعلومات في تحت عنوان
+
+96
+00:07:17,550 --> 00:07:20,390
+solution used in medical laboratory كل المحاليل
+
+97
+00:07:20,390 --> 00:07:23,250
+اللي تستخدم في الأختبارات الطبية راح نحكي عنها
+
+98
+00:07:24,320 --> 00:07:28,280
+أنواعها كمان راح نحكي عن حاجة اسمها automatic by
+
+99
+00:07:28,280 --> 00:07:31,840
+-bet use and calibration شو يعني automatic by-bet؟
+
+100
+00:07:31,840 --> 00:07:35,480
+اذا اخدتوا انتوا معاهم ال .. ال .. الكيميا
+
+101
+00:07:35,480 --> 00:07:40,860
+الأساسية ال .. أكيد مر معاكم اللي هي لفظ الصحاحة
+
+102
+00:07:40,860 --> 00:07:43,360
+أو ال by-betاللي كنتوا تستخدمها to transfer
+
+103
+00:07:43,360 --> 00:07:48,240
+volumes يعني لتنقلها حجوم طبعا فاتعلمتوا كيف ال
+
+104
+00:07:48,240 --> 00:07:52,860
+use ليلها كيف استخدامها و أيضا كيف ان انا اعمل لها
+
+105
+00:07:52,860 --> 00:07:58,480
+calibration كيف يعني طبعا استخدامها طبعا احنا في
+
+106
+00:07:58,480 --> 00:08:02,920
+المعامل هنا بت .. بنحكي عن نوع من ال bybit اللي هو
+
+107
+00:08:02,920 --> 00:08:05,900
+متقدم استخدامه نوع عامة اللي هو automatic bybit
+
+108
+00:08:06,330 --> 00:08:09,130
+طبعا انا ليش بستخدم ال bibet ال automatic اول حاجة
+
+109
+00:08:09,130 --> 00:08:13,250
+لإنه بدي درجة عالية من ال accuracy و ده درجة عالية
+
+110
+00:08:13,250 --> 00:08:17,330
+من ال precision الصحة و الدقة ليش؟ انا معنية انه
+
+111
+00:08:17,330 --> 00:08:21,230
+انا استخدم bibet دقيقة bibet accurate and precise
+
+112
+00:08:21,230 --> 00:08:27,480
+لحتى انقل حجم بدقةومن ثم اجري تفاعل دقيق احصل على
+
+113
+00:08:27,480 --> 00:08:31,340
+نتائج صحيحة وبالتالي انا اعبر عن صحة البقى المريض
+
+114
+00:08:31,340 --> 00:08:36,180
+او اعبر عن حالته بشكل صحيح وهك انا بساهم ان انا
+
+115
+00:08:36,180 --> 00:08:41,540
+بانقاد روح هذا المريض ايضا نحتاج ل ال calibration
+
+116
+00:08:41,540 --> 00:08:46,160
+يعني ان انا كيف اعيد تهيئة او ضبط هاي ال vibe في
+
+117
+00:08:46,160 --> 00:08:50,280
+حال لو كان اكتشفت ان فيها خلل او error كيف انا بدي
+
+118
+00:08:50,280 --> 00:08:55,690
+اضبطهارح نحكي أيضا عن ال blood blood sample
+
+119
+00:08:55,690 --> 00:08:59,790
+collection and processing كيف أنا أسحب عينة دم من
+
+120
+00:08:59,790 --> 00:09:04,850
+مريض سواء كان هذا المريض adult سواء يعني شخص كبير
+
+121
+00:09:04,850 --> 00:09:12,510
+بالعمر بالغ أو شخص newborn طفل حديث الولادة من وين
+
+122
+00:09:12,510 --> 00:09:15,870
+أنا باخد العينة سواء كانت من الوريد أو من ال skin
+
+123
+00:09:15,870 --> 00:09:19,570
+كيف طريقة أخذه العينة كل هذا رح نتعلمه في ال blood
+
+124
+00:09:19,570 --> 00:09:24,730
+sample collection و أيضارح نتعرف كيف أنا بتعامل مع
+
+125
+00:09:24,730 --> 00:09:28,670
+عينة الدم هذه يعني في بعض الفحوصات بتحتاج أنه أنا
+
+126
+00:09:28,670 --> 00:09:33,770
+بدي أفصل هذه العينة لحتى أحصل على مثلا جزء من
+
+127
+00:09:33,770 --> 00:09:38,090
+مكونات المادة يعني انا لو بدي فحص بيشتغل على
+
+128
+00:09:38,090 --> 00:09:42,530
+بلازمة أو على خلايا فانا محتاجة لفحص بيشتغل على
+
+129
+00:09:42,530 --> 00:09:48,020
+جزء من مكونات العينة لحتىأكمل عليه احيانا في
+
+130
+00:09:48,020 --> 00:09:50,920
+فحوصات بتحتاج ال whole blood يعني الدم ككل اشتغل
+
+131
+00:09:50,920 --> 00:09:54,000
+عليه فهنا محتاجة ان انا مثلا فقط ان انا اخزن
+
+132
+00:09:54,000 --> 00:09:58,320
+العينة او احتفظ فيها لحين استخدامها تحت ظروف معينة
+
+133
+00:09:58,320 --> 00:10:02,140
+انه ما تخرب العينة فهد كله راح نتعلمه في ال blood
+
+134
+00:10:02,140 --> 00:10:04,720
+sample collection and processing حكينا انه
+
+135
+00:10:04,720 --> 00:10:08,140
+المعلومات ان شاء الله راح تكون بالنسبة ل .. يعني
+
+136
+00:10:08,140 --> 00:10:11,020
+لما ن .. نفردها و نحكي عنها بالتفصيل ان شاء الله
+
+137
+00:10:11,020 --> 00:10:15,760
+ماراح يكون في اشيصعب أو يكون في اشي غامض كلش ان
+
+138
+00:10:15,760 --> 00:10:20,160
+شاء الله رح نبسطه و نسهله لإلكم بحيث انه تستفيدوا
+
+139
+00:10:20,160 --> 00:10:24,300
+رح نحكي عن بعض الأدوات sorry مش الأدوات الأجهزة
+
+140
+00:10:24,300 --> 00:10:28,040
+اللي موجودة داخل المختبر لبقى instruments رح نحكي
+
+141
+00:10:28,040 --> 00:10:32,580
+عن ال microscope أنواعه طريقة استخدامه طريقة
+
+142
+00:10:32,580 --> 00:10:38,760
+التعامل معاه مبدأ عمله أدواته مكوناته sorry أجزاءه
+
+143
+00:10:39,070 --> 00:10:44,530
+و كتطبيق على استخدام ال microscope راح ناخد طريقة
+
+144
+00:10:44,530 --> 00:10:48,090
+عد ال white pieces و ال RPCs كيف انا اعد خلايا
+
+145
+00:10:48,090 --> 00:10:51,290
+الدم البيضاء عدد خلايا الدم البيضاء و خلايا الدم
+
+146
+00:10:51,290 --> 00:10:56,830
+الحمراء في عينة الدم تمام اذا بعد ما احصل على عينة
+
+147
+00:10:56,830 --> 00:10:59,490
+الدم بدي اتعلم كيف اعد ال white pieces و كيف اعد
+
+148
+00:10:59,490 --> 00:11:05,110
+ال RPCsبدي أتعلم كيف أجيب hematocrit يعني كيف
+
+149
+00:11:05,110 --> 00:11:09,550
+أشتغل على ال hematocrit ال hematocrit هو عبارة عن
+
+150
+00:11:09,550 --> 00:11:15,750
+النسبة أو المساحة أو ال volume اللي بيحتلها ال
+
+151
+00:11:15,750 --> 00:11:20,050
+RBCs بالنسبة لل whole sample يعني كمية يعني الحجم
+
+152
+00:11:20,050 --> 00:11:23,750
+اللي بيحتله ال RBCs أو خلايا الدم الحمراء بالنسبة
+
+153
+00:11:23,750 --> 00:11:29,500
+للحجم اللي بيحتله العينة كاملةفطبعا هذا أيضا يجري
+
+154
+00:11:29,500 --> 00:11:34,600
+على عينة الدم بتطبيق جهاز اسمه centrifuge راح ناخد
+
+155
+00:11:34,600 --> 00:11:37,900
+ايضا فحص اللي هو ال hemoglobin concentration
+
+156
+00:11:37,900 --> 00:11:42,380
+determination يعني انا بدي احسب تركيز بروتين ال
+
+157
+00:11:42,380 --> 00:11:46,230
+hemoglobinاللي هو يعني بالبلدي أو بالعربي بنحكي
+
+158
+00:11:46,230 --> 00:11:50,750
+عنه قوة الدم، بدي أقيص قوة الدم فبدي أقيص تركيز ال
+
+159
+00:11:50,750 --> 00:11:53,710
+homoglobin اللي هو عبارة عن ال oxygen carrying
+
+160
+00:11:53,710 --> 00:11:57,290
+protein اللي هو ال protein اللي ناقل ال oxygen
+
+161
+00:11:57,290 --> 00:12:02,650
+داخل خلايا الدم الحمراء و بهك تقريبا تقريبا أنا
+
+162
+00:12:02,650 --> 00:12:09,290
+بكون طبعا مع محاضرة ال blood indices يعني محاضرة
+
+163
+00:12:09,290 --> 00:12:16,800
+نظرية راح نحكي عنهاهيك تقريبا انا بكون عملت حاجة
+
+164
+00:12:16,800 --> 00:12:22,820
+اسمها CBC بس اخلص هاي المواضيع و تشتغلوها بتكونوا
+
+165
+00:12:22,820 --> 00:12:28,640
+اشتغلتوا CBC ولكن يدوي، اش يعني CBC؟ يعني complete
+
+166
+00:12:28,640 --> 00:12:35,460
+blood countيعني عد كامل أو فحص شامل لدم، تمام؟ هذا
+
+167
+00:12:35,460 --> 00:12:40,340
+الفحص أنتوا في معامل علم الدم و الدم التشقيسي يعني
+
+168
+00:12:40,340 --> 00:12:45,020
+في المختبرات التخصص القادمة يعني في سنة تالتة و
+
+169
+00:12:45,020 --> 00:12:50,300
+رابعة راح تشتغلوا باستخدام أجهزة هذا الفحص يعني
+
+170
+00:12:50,300 --> 00:12:54,960
+فقط الدخل عين على الجهاز و الجهاز يطلع لك كل الاشي
+
+171
+00:12:54,960 --> 00:13:02,240
+مقروه جاهزلكن هنا بتشتغلوا بإيدكم و هذا هو ميزة
+
+172
+00:13:02,240 --> 00:13:06,240
+المصاق .. ميزة المصاق أنه أنتوا بتاخدوا ال ..
+
+173
+00:13:06,240 --> 00:13:11,420
+الاشي بتعمق أكتر و بتاخدوا الأساسيات فعليا أنه
+
+174
+00:13:11,420 --> 00:13:15,220
+أنتوا تشتغليه بإيدك أفضل بكتير من ما أنه العينة
+
+175
+00:13:15,220 --> 00:13:18,980
+يشتغلها جهاز .. الجهاز أنت ما بتعرفي هو كيف اشتغل
+
+176
+00:13:18,980 --> 00:13:22,040
+ولا .. ولا على أي مبدأ اعتمد لكن لما أنتوا تشتغلي
+
+177
+00:13:22,040 --> 00:13:27,010
+بإيدكبتكوني عارفة كل كبيرة و صغيرة كل محلول ليش
+
+178
+00:13:27,010 --> 00:13:30,630
+استخدمتيه و إيش فايدته و إيش مبدأ العمل و ليش
+
+179
+00:13:30,630 --> 00:13:33,810
+تخطيطات اللي استخدمتيها و إيش القوانين اللي
+
+180
+00:13:33,810 --> 00:13:37,610
+بتطبيها لحتى تحصل على النتيجة النهائية ففي النهاية
+
+181
+00:13:37,610 --> 00:13:41,950
+بتكوني أنتي ال counter لل blood أحسن يعني أنتي
+
+182
+00:13:41,950 --> 00:13:48,190
+أحسن من .. من الجهاز بتشتغليفلهيك يعني الجهة ..
+
+183
+00:13:48,190 --> 00:13:53,670
+المادة تبعتنا تأسيسية و أساسية للمثاقات الأخرى و
+
+184
+00:13:53,670 --> 00:13:58,150
+.. اذا انا .. اذا بتلاحظوا يعني تركت بعض المواضيع
+
+185
+00:13:58,150 --> 00:14:02,130
+لإن المواضيع اللي عرجت عليها كانت شوي مترابطة راح
+
+186
+00:14:02,130 --> 00:14:05,570
+نحكي عن جهة M فحص ال glucose test راح تاخدوا فحص
+
+187
+00:14:05,570 --> 00:14:09,450
+قياس مستوى السكر في الدم و تقرؤوها على جهاز ال
+
+188
+00:14:09,450 --> 00:14:12,480
+spectrophotometer اللي هو المطياف الدوئياللي هو
+
+189
+00:14:12,480 --> 00:14:16,900
+عبارة عن جهاز بيقيص لتركيز كتير من ال parameters
+
+190
+00:14:16,900 --> 00:14:21,440
+أو المواد اللي موجودة داخل عينات الدم يعني بجريلي
+
+191
+00:14:21,440 --> 00:14:25,560
+بحوصات كتير في معمل في السنة الرابعة اسمه ال
+
+192
+00:14:25,560 --> 00:14:29,260
+clinical chemistry اللي هو الكيميا الطبية راح يكون
+
+193
+00:14:29,260 --> 00:14:34,710
+كل شغلكم على هذا الجهاز ويعني إذا سمعتوايعني
+
+194
+00:14:34,710 --> 00:14:38,450
+فحوصات وظائف الأعضاء زي وظائف الكبد وظائف الكلا
+
+195
+00:14:38,450 --> 00:14:44,030
+وظائف القلب وظائف ال .. كل جزء من .. جزء من أجزاء
+
+196
+00:14:44,030 --> 00:14:49,110
+الإنسان يجرى طبعا على .. يعني هذه فحوصات هتجرى على
+
+197
+00:14:49,110 --> 00:14:52,710
+جهاز ال spectrophotometer فإحنا هنا دورنا أنه أنا
+
+198
+00:14:52,710 --> 00:14:56,250
+أعرف إيش يعني جهاز ال spectrophotometer إيش مبدأ
+
+199
+00:14:56,250 --> 00:15:02,230
+أعمله، إيش مكوناته وطريقة استخدامه و أطبق كتطبيق
+
+200
+00:15:02,230 --> 00:15:06,450
+بسيط عليه فحص السكرعشان لما أنتقل على المختبر
+
+201
+00:15:06,450 --> 00:15:10,910
+التخصص اللي هو اللي .. اللي بيعتمد بدرجة أولى على
+
+202
+00:15:10,910 --> 00:15:13,610
+استخدام ال spectrophotometer يكون عندي خلفية
+
+203
+00:15:13,610 --> 00:15:20,650
+ومعرفة عنه تمام حكينا أنه فحص ال hematocrit
+
+204
+00:15:20,650 --> 00:15:23,570
+هنستخدمله جهاز ال centrifuge اللي هو جهاز التارد
+
+205
+00:15:23,570 --> 00:15:28,650
+المركزي راح نحكي عنه ال estimated sedimentation
+
+206
+00:15:28,650 --> 00:15:32,190
+rate اللي هو فحص الترسيب أكيد سمعتوا عن حاجة اسمها
+
+207
+00:15:32,190 --> 00:15:37,830
+فحص الترسيب بالعربيراح أيضا تشتغلوا بهذه .. بهذا
+
+208
+00:15:37,830 --> 00:15:42,710
+المصاق بعد هيك في نهاية المصاق راح نعلمكم كيف
+
+209
+00:15:42,710 --> 00:15:46,450
+تحضروا blood smear preparation إيش يعني blood
+
+210
+00:15:46,450 --> 00:15:50,030
+smear preparation يعني أنه أنا أاخد جزء من عينة
+
+211
+00:15:50,030 --> 00:15:55,530
+الدم أفردها على شريحة زجاجية و أسبغها و أحاول تكون
+
+212
+00:15:55,530 --> 00:16:00,290
+واضحة لرؤية الخلايا تحت ال microscope طبعا .. طبعا
+
+213
+00:16:00,290 --> 00:16:05,850
+أنا ليش بعمل هاي الشغلة أو هذا التحضيرلإنه يعني
+
+214
+00:16:05,850 --> 00:16:11,530
+كتير أنواع من ال anemia و من ال leukemia يعني فقر
+
+215
+00:16:11,530 --> 00:16:15,190
+الدم، أنواع فقر الدم و أنواع سرطانات الدم عفوا
+
+216
+00:16:15,190 --> 00:16:20,770
+الله و إياكم بتتم تشقيصها من رؤية شكل الخلايا على
+
+217
+00:16:20,770 --> 00:16:24,640
+ال microscopeفأنا لازم أكون بعرف كيف أحضر مسحة
+
+218
+00:16:24,640 --> 00:16:29,280
+الدم على شريحة، كيف أصبغها وكيف أنا أشوفها تحت ال
+
+219
+00:16:29,280 --> 00:16:33,600
+microscope و أتعرف على الخلايا علشان بعد هيك لما
+
+220
+00:16:33,600 --> 00:16:41,120
+نتعلم في المواد التخصصي بتانية ان .. ان نربط أنواع
+
+221
+00:16:41,560 --> 00:16:46,460
+خلايا abnormal يعني مش طبيعية بمرض معين أول ما
+
+222
+00:16:46,460 --> 00:16:49,840
+أشوف الخلية هذه اللي مش .. مش تمام اللي هي
+
+223
+00:16:49,840 --> 00:16:54,360
+abnormal اه ييجي في بالي المرض الفلاني فطبعا يعني
+
+224
+00:16:54,360 --> 00:16:58,820
+مش .. لازم تمروا بمراحل اللي هي التعلم مراحل تعلم
+
+225
+00:16:58,820 --> 00:17:05,100
+تحضير المسحة مع مراحل تعلم صبغ المسحة و أيضا رؤية
+
+226
+00:17:05,100 --> 00:17:09,360
+الخلايا على الأقل اللي تشوفه normalعشان لما بقعد
+
+227
+00:17:09,360 --> 00:17:12,780
+هيك في المساقات أخرى تشوفوا ال abnormal تكونوا
+
+228
+00:17:12,780 --> 00:17:16,580
+جادرين يتميزوا ما بين الطبيعي والغير طبيعي، هذا
+
+229
+00:17:16,580 --> 00:17:21,700
+بالنسبة لالمواضيع اللي احنا اتناولها خلال ال ..
+
+230
+00:17:22,430 --> 00:17:30,070
+المصاق هنا فعليا زي ما انتوا شايفين في مواضيع هي
+
+231
+00:17:30,070 --> 00:17:36,010
+مواضيع نظرية بحتة وفي مواضيع لأ هي نظرية وعملية
+
+232
+00:17:36,010 --> 00:17:40,050
+علشان هيك بتلاحظوا ان المصاق لما انتوا جيتوا سجلته
+
+233
+00:17:40,050 --> 00:17:45,210
+عبارة عن مقدمة في التحليل الطبية مناقشة يعني بينا
+
+234
+00:17:45,210 --> 00:17:49,110
+.. احنا راح نحكي في تحت هذا ال band كل المواضيع
+
+235
+00:17:49,110 --> 00:17:54,280
+اللي هي نظرية بحتة مش فيها عملي خالص بيكونو راح
+
+236
+00:17:54,280 --> 00:17:57,840
+نحكي تحت band المقدمة في التحليل اللي هو العملي
+
+237
+00:17:57,840 --> 00:18:03,580
+المواضيع العملية هلأ احنا راح نغطي جزء النظري
+
+238
+00:18:03,580 --> 00:18:08,940
+الباحن و نظري العملي و راح ان شاء الله يعني زي ما
+
+239
+00:18:08,940 --> 00:18:12,140
+صار في الفصول .. في الفصل السابق احنا يعني أعطينا
+
+240
+00:18:12,140 --> 00:18:16,600
+المثقات بعد هي أعطينا شهر استدراكي تعويضي ان كل
+
+241
+00:18:16,600 --> 00:18:22,550
+المعامل عوضناها يعني جيبنا الطالبات على ال ..معامل
+
+242
+00:18:22,550 --> 00:18:26,990
+ووزعناهم جروبات بحيث ما يكون فيه تكدس، يكون فيه
+
+243
+00:18:26,990 --> 00:18:32,250
+تباعد، سبل وقاية وخلينهم يشتغلوا الجزئية العملي
+
+244
+00:18:32,250 --> 00:18:38,550
+اللي موجودة معاهم، فيعني أنا أتوقع ويعني القسم
+
+245
+00:18:38,550 --> 00:18:42,310
+أكيد يعني راح ان شاء الله يطبق هذا ال .. الاشياء
+
+246
+00:18:42,780 --> 00:18:48,880
+فالجزئية العملي راح ن .. ن .. نأجلها لحين عودتنا
+
+247
+00:18:48,880 --> 00:18:54,080
+ان شاء الله او لحين وضع خطة للتعويض العملي تمام؟
+
+248
+00:18:54,080 --> 00:18:58,280
+ولكن لحتى ما يسرقنا الوقت احنا راح نظل مستمرين على
+
+249
+00:18:58,280 --> 00:19:01,920
+الأقل لنغطي الجزئية النظرى من خلال المحادرات ال
+
+250
+00:19:01,920 --> 00:19:07,230
+onlineزي ما أنتوا عارفين طبيعة المساق هو ساعة
+
+251
+00:19:07,230 --> 00:19:11,590
+مناقشة و تلت ساعات lab يعني كان مطلوب منكم لو
+
+252
+00:19:11,590 --> 00:19:14,350
+أنتوا بتيجوا على المختبرات و بتداوموا كان مطلوب
+
+253
+00:19:14,350 --> 00:19:18,270
+منكم و أربع ساعات تلتزمي بال .. بيوتعطيها من وقتك
+
+254
+00:19:18,270 --> 00:19:23,730
+حق للمادة فأنا هلأ بدي أطلب منكم أنه اتحضرولي يعني
+
+255
+00:19:23,730 --> 00:19:29,290
+تتفقوا على ساعة أنزللكم فيها أو يعني توقيت أنزللكم
+
+256
+00:19:29,290 --> 00:19:35,240
+فيهالمحاضرة النظرية اللي هي المناقشة و المحاضرة
+
+257
+00:19:35,240 --> 00:19:41,180
+العملية راح نشرح الجزء النظري منها و نسيب العملي
+
+258
+00:19:41,180 --> 00:19:47,840
+لحين التعويض و جزء ساعة لإن .. إن نكون موجودين
+
+259
+00:19:47,840 --> 00:19:53,820
+كلنا في نفس ذات اللحظة على موقع ال BB اللي هو يعني
+
+260
+00:19:53,820 --> 00:19:59,260
+موقع ال online تباع ال model أو ال ..ال zoom او
+
+261
+00:19:59,260 --> 00:20:03,660
+موقع class, google meeting, اي موقع انتوا بتختاروه
+
+262
+00:20:03,660 --> 00:20:07,660
+بيكون فيه اجماعة ليه انا ماعندي مشكلة و بحيث انه
+
+263
+00:20:07,660 --> 00:20:11,660
+نكون كلنا موجودين لو مافهمتوا اشي اثناء شرح
+
+264
+00:20:11,660 --> 00:20:15,640
+المحاضرة او تسجيل المحاضرة بنفتح المحاضرة بنرجع ل
+
+265
+00:20:15,640 --> 00:20:21,660
+ال slides بنعرج عليها بنفهمهاإذا في عندكم أي
+
+266
+00:20:21,660 --> 00:20:25,980
+استفسار أو أي سؤال بتجاوب .. يعني بس تسألوني و
+
+267
+00:20:25,980 --> 00:20:30,340
+بجاوبكم عليه ممكن يكون في نقاش و النقاش طبعا معروف
+
+268
+00:20:30,340 --> 00:20:37,480
+أن هو يثري المعلومات ف .. و بعمق الفهم فيعني هدول
+
+269
+00:20:37,480 --> 00:20:41,300
+التلت ساعات لغن عنهم لازم أنه .. يعني يكون في عندك
+
+270
+00:20:41,660 --> 00:20:46,940
+ساعة تسمع فيها تسجيل المحاضرة النظري .. المحاضرة
+
+271
+00:20:46,940 --> 00:20:51,720
+العملي ساعة أيضا و ساعة النقاش. هلأ نيجي لتوزيع
+
+272
+00:20:51,720 --> 00:20:56,980
+الدرجات 30% من المادة راح يكون على ال quizzes و ال
+
+273
+00:20:56,980 --> 00:21:01,800
+midterm exam يعني راح أعطيكم مثلابعد ما نخلص تلت
+
+274
+00:21:01,800 --> 00:21:05,840
+معامل مثلا او اربعة رح احدد بالاتفاق معاكم على
+
+275
+00:21:05,840 --> 00:21:11,700
+موعد و توقيت ل quiz بنزللكم الأسئلة على ال model
+
+276
+00:21:11,700 --> 00:21:16,440
+بتفوتوا في موعد ال quiz بتقدمي ال quiz و بتطلعي
+
+277
+00:21:16,440 --> 00:21:19,620
+ففي النهاية انا بحسبلكم على مجموع ال quiz ذات و
+
+278
+00:21:19,620 --> 00:21:23,540
+الامتحان النصفي تلاتين درجةاربعين درجة على ال
+
+279
+00:21:23,540 --> 00:21:27,460
+final exam و تلاتين على ما على ال lab work و ال
+
+280
+00:21:27,460 --> 00:21:30,880
+experiment هذا في حالة لما يكون نرجع على المختبرات
+
+281
+00:21:30,880 --> 00:21:35,240
+و نطبق التجارب راح يكون في تلاتين درجة على التزامك
+
+282
+00:21:35,240 --> 00:21:40,000
+بالدوام أثناء التعويض التزامك بالمعرفة .. بالتجربة
+
+283
+00:21:40,000 --> 00:21:45,420
+ب .. فهمك للتجربة شغلك و حرصك على أنك انت تشتغلي و
+
+284
+00:21:45,420 --> 00:21:49,180
+تسلمي نتائج ما .. مش راح يكون فيه تقييم على صحة
+
+285
+00:21:49,180 --> 00:21:52,330
+النتائج لإن انتوا جايين تتعلمواو أنتوا بتفوتوا
+
+286
+00:21:52,330 --> 00:21:56,930
+مختبرات طبية لأول مرة فمش رح أجي أحقق .. أقيمك أن
+
+287
+00:21:56,930 --> 00:22:00,610
+والله نتيجتك اللي سلمتينيها صح أو خطأ و أنت جاي
+
+288
+00:22:00,610 --> 00:22:04,910
+تتعلمي لأ رح أسال .. رح .. رح أقايمك في حال أنه
+
+289
+00:22:04,910 --> 00:22:09,570
+يعني ال attitude تبعك يعني أن أنت هل كنت على
+
+290
+00:22:09,570 --> 00:22:14,670
+استعداد أنك تتعلمي ولا كنت care less سلمتيني نتائج
+
+291
+00:22:14,670 --> 00:22:18,490
+ولا ما سلمتي اشتغلتي ولا ما اشتغلتي طبقت التعليمات
+
+292
+00:22:18,490 --> 00:22:22,760
+اللي أنا حكيتهابحدافيرها ولا لأ ال .. ال .. مدى
+
+293
+00:22:22,760 --> 00:22:29,420
+الالتزام و مدى الاهتمام بالحضور و بالمدى الجدية
+
+294
+00:22:29,420 --> 00:22:33,960
+داخل المختبر هدول اللي عليهم تلاتين علامة مش صحة
+
+295
+00:22:33,960 --> 00:22:37,940
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+301
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+ال facebook و حساب ال instagram و ال whatsapp على
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+319
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diff --git a/PL9fwy3NUQKwY9jl8xw-bWkVZkWcGGyspd/xD3hYPD-Pa0_raw.json b/PL9fwy3NUQKwY9jl8xw-bWkVZkWcGGyspd/xD3hYPD-Pa0_raw.json
new file mode 100644
index 0000000000000000000000000000000000000000..98b125d464f1d8b3094547867e42971dff38357a
--- /dev/null
+++ b/PL9fwy3NUQKwY9jl8xw-bWkVZkWcGGyspd/xD3hYPD-Pa0_raw.json
@@ -0,0 +1 @@
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new file mode 100644
index 0000000000000000000000000000000000000000..206857bf8e1f88fb3425f4ffc60000e1e62e2b51
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@@ -0,0 +1 @@
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1807.43, "word": " ممكن", "probability": 0.97216796875}, {"start": 1807.43, "end": 1807.65, "word": " أنت", "probability": 0.5548095703125}, {"start": 1807.65, "end": 1808.11, "word": " تعقمي", "probability": 0.69525146484375}, {"start": 1808.11, "end": 1808.51, "word": " بشكل", "probability": 0.9962565104166666}, {"start": 1808.51, "end": 1808.93, "word": " دائي", "probability": 0.8453776041666666}, {"start": 1808.93, "end": 1809.37, "word": " بس", "probability": 0.9814453125}, {"start": 1809.37, "end": 1810.25, "word": " ماتجربي", "probability": 0.83447265625}, {"start": 1810.25, "end": 1810.63, "word": " عملية", "probability": 0.9837239583333334}, {"start": 1810.63, "end": 1811.03, "word": " السحب", "probability": 0.9396158854166666}, {"start": 1811.03, "end": 1811.27, "word": " إلا", "probability": 0.96533203125}, {"start": 1811.27, "end": 1811.53, "word": " لما", "probability": 0.958984375}, {"start": 1811.53, "end": 1812.11, "word": " اتجربيها", "probability": 0.8768310546875}, 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0.810302734375}, {"start": 1818.39, "end": 1820.09, "word": " يعني", "probability": 0.79296875}, {"start": 1820.09, "end": 1820.41, "word": " whatever", "probability": 0.923828125}], "temperature": 1.0}, {"id": 80, "seek": 184870, "start": 1820.88, "end": 1848.7, "text": "تمام؟ إذا هك بنكون خلصنا جزئية ال pheloptomy اللي هو سحب عين الدم من الوريد تمام؟ هذا ال .. الجزء .. أو هذا ال .. الفيديو التعليمي اللي أنا سجلتلكم إياه ان شاء الله يكون .. تكونوا استفدته من خلاله و يعطيكم العافية ان شاء الله بنسجللكم فيديوهات أخرى بتوضح عملية ال skin puncture", "tokens": [39237, 10943, 22807, 11933, 15730, 8032, 4117, 44945, 30544, 16490, 1211, 9381, 8315, 10874, 11622, 19986, 10632, 2423, 903, 338, 5747, 8488, 13672, 1829, 31439, 8608, 5016, 3555, 6225, 9957, 32748, 2304, 9154, 2423, 13063, 25708, 46811, 10943, 22807, 23758, 2423, 4386, 25724, 11622, 38207, 4386, 34051, 23758, 2423, 4386, 27188, 1829, 16254, 2407, 16712, 3615, 20292, 2304, 1829, 13672, 1829, 41850, 8608, 7435, 1211, 2655, 23275, 2304, 11933, 25528, 3224, 16472, 13412, 16606, 21984, 7251, 30544, 4386, 6055, 30544, 14407, 44713, 5172, 3215, 47395, 9154, 16490, 1211, 6027, 3224, 4032, 37495, 9566, 1829, 24793, 18863, 31845, 10632, 16472, 13412, 16606, 21984, 44945, 3794, 7435, 1211, 23275, 2304, 8978, 16254, 2407, 3224, 9307, 5551, 34740, 7578, 39894, 2407, 11242, 5016, 6225, 42213, 10632, 2423, 3178, 27006, 540], "avg_logprob": -0.14343261532485485, "compression_ratio": 1.797709923664122, "no_speech_prob": 7.748603820800781e-06, "words": [{"start": 1820.88, "end": 1821.74, "word": "تمام؟", "probability": 0.6734212239583334}, {"start": 1821.74, "end": 1821.96, "word": " إذا", "probability": 0.71630859375}, {"start": 1821.96, "end": 1822.44, "word": " هك", "probability": 0.5491943359375}, {"start": 1822.44, "end": 1823.08, "word": " بنكون", "probability": 0.98095703125}, {"start": 1823.08, "end": 1823.72, "word": " خلصنا", "probability": 0.9752197265625}, {"start": 1823.72, "end": 1824.44, "word": " جزئية", "probability": 0.975830078125}, {"start": 1824.44, "end": 1825.2, "word": " ال", "probability": 0.8642578125}, {"start": 1825.2, "end": 1826.04, "word": " pheloptomy", "probability": 0.64306640625}, {"start": 1826.04, "end": 1826.36, "word": " اللي", "probability": 0.773681640625}, {"start": 1826.36, "end": 1826.56, "word": " هو", "probability": 0.97705078125}, {"start": 1826.56, "end": 1826.96, "word": " سحب", "probability": 0.9684244791666666}, {"start": 1826.96, "end": 1827.32, "word": " عين", "probability": 0.891845703125}, {"start": 1827.32, "end": 1827.76, "word": " الدم", "probability": 0.861083984375}, {"start": 1827.76, "end": 1828.1, "word": " من", "probability": 0.9951171875}, {"start": 1828.1, "end": 1829.4, "word": " الوريد", "probability": 0.9405924479166666}, {"start": 1829.4, "end": 1830.58, "word": " تمام؟", "probability": 0.8447265625}, {"start": 1830.58, "end": 1831.14, "word": " هذا", "probability": 0.8232421875}, {"start": 1831.14, "end": 1831.82, "word": " ال", "probability": 0.67529296875}, {"start": 1831.82, "end": 1832.48, "word": " ..", "probability": 0.9560546875}, {"start": 1832.48, "end": 1833.72, "word": " الجزء", "probability": 0.8367513020833334}, {"start": 1833.72, "end": 1833.8, "word": " ..", "probability": 0.52099609375}, {"start": 1833.8, "end": 1833.96, "word": " أو", "probability": 0.57177734375}, {"start": 1833.96, "end": 1834.16, "word": " هذا", "probability": 0.91796875}, {"start": 1834.16, "end": 1834.38, "word": " ال", "probability": 0.87890625}, {"start": 1834.38, "end": 1834.38, "word": " ..", "probability": 0.6796875}, {"start": 1834.38, "end": 1834.7, "word": " الفيديو", "probability": 0.8465576171875}, {"start": 1834.7, "end": 1835.18, "word": " التعليمي", "probability": 0.97587890625}, {"start": 1835.18, "end": 1835.3, "word": " اللي", "probability": 0.9765625}, {"start": 1835.3, "end": 1835.46, "word": " أنا", "probability": 0.896484375}, {"start": 1835.46, "end": 1836.1, "word": " سجلتلكم", "probability": 0.9668782552083334}, {"start": 1836.1, "end": 1836.36, "word": " إياه", "probability": 0.7884928385416666}, {"start": 1836.36, "end": 1836.5, "word": " ان", "probability": 0.52197265625}, {"start": 1836.5, "end": 1836.64, "word": " شاء", "probability": 0.962158203125}, {"start": 1836.64, "end": 1836.74, "word": " الله", "probability": 0.9501953125}, {"start": 1836.74, "end": 1837.04, "word": " يكون", "probability": 0.906005859375}, {"start": 1837.04, "end": 1837.08, "word": " ..", "probability": 0.7197265625}, {"start": 1837.08, "end": 1837.42, "word": " تكونوا", "probability": 0.74755859375}, {"start": 1837.42, "end": 1838.02, "word": " استفدته", "probability": 0.882568359375}, {"start": 1838.02, "end": 1839.36, "word": " من", "probability": 0.99658203125}, {"start": 1839.36, "end": 1840.06, "word": " خلاله", "probability": 0.9921875}, {"start": 1840.06, "end": 1840.58, "word": " و", "probability": 0.9873046875}, {"start": 1840.58, "end": 1842.0, "word": " يعطيكم", "probability": 0.8863525390625}, {"start": 1842.0, "end": 1842.68, "word": " العافية", "probability": 0.9903971354166666}, {"start": 1842.68, "end": 1843.24, "word": " ان", "probability": 0.7275390625}, {"start": 1843.24, "end": 1843.38, "word": " شاء", "probability": 0.9951171875}, {"start": 1843.38, "end": 1843.64, "word": " الله", "probability": 0.95947265625}, {"start": 1843.64, "end": 1845.1, "word": " بنسجللكم", "probability": 0.9025065104166666}, {"start": 1845.1, "end": 1845.66, "word": " فيديوهات", "probability": 0.96669921875}, {"start": 1845.66, "end": 1846.12, "word": " أخرى", "probability": 0.986328125}, {"start": 1846.12, "end": 1846.8, "word": " بتوضح", "probability": 0.9686279296875}, {"start": 1846.8, "end": 1847.56, "word": " عملية", "probability": 0.9837239583333334}, {"start": 1847.56, "end": 1847.8, "word": " ال", "probability": 0.80224609375}, {"start": 1847.8, "end": 1848.12, "word": " skin", "probability": 0.9296875}, {"start": 1848.12, "end": 1848.7, "word": " puncture", 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\ No newline at end of file
diff --git a/PL9fwy3NUQKwY9jl8xw-bWkVZkWcGGyspd/yuOrEl54r0w_raw.srt b/PL9fwy3NUQKwY9jl8xw-bWkVZkWcGGyspd/yuOrEl54r0w_raw.srt
new file mode 100644
index 0000000000000000000000000000000000000000..c1f674c56b208750aaf00c54c2d87da2a69b649b
--- /dev/null
+++ b/PL9fwy3NUQKwY9jl8xw-bWkVZkWcGGyspd/yuOrEl54r0w_raw.srt
@@ -0,0 +1,1592 @@
+1
+00:00:03,800 --> 00:00:07,540
+السلام عليكم ورحمة الله وبركاته طالبات العزيزات
+
+2
+00:00:07,540 --> 00:00:11,800
+اليوم ان شاء الله راح نستعرض مع بعض فيديو منزلي
+
+3
+00:00:11,800 --> 00:00:16,100
+صورته أنا بالمنزل و بعد توفير الأدوات اللازمة و
+
+4
+00:00:16,100 --> 00:00:21,160
+هذا الفيديو بيشرح كيفية سحب عين الدم من منطقة
+
+5
+00:00:21,160 --> 00:00:24,500
+الوريد تمام؟ راح نسحب عين الدم من منطقة ال
+
+6
+00:00:24,500 --> 00:00:30,140
+antecubital و راح نذكر أيضا بعض النقاط المهمة
+
+7
+00:00:30,140 --> 00:00:34,460
+المتعلقة بعملية السحبهذه النقاط المهمة احنا
+
+8
+00:00:34,460 --> 00:00:39,360
+ذكرناها في المحاضرة النظرية وحكينا انها هي تعليمات
+
+9
+00:00:39,360 --> 00:00:44,600
+مهمة لازم نتبعها أثناء عملية السحب و .. لكن طبعا
+
+10
+00:00:44,600 --> 00:00:48,280
+ما .. ما .. ال .. الاشي ما بيكون متخيل بدون ما
+
+11
+00:00:48,280 --> 00:00:52,220
+انطبق عملي و بدون ما نشوفه بعينينالهيك أنا اليوم
+
+12
+00:00:52,220 --> 00:00:57,420
+قررت أصورلكم هذا ال video لحتى أقرب الأشي لأذهانكم
+
+13
+00:00:57,420 --> 00:01:01,900
+تشوفوا الأشي بعينيكم لما نذكر المعلومة و نطبقها
+
+14
+00:01:01,900 --> 00:01:07,540
+تعرفوا إيش كان المقصود بهي التعليمات أثناء ذكرها
+
+15
+00:01:07,540 --> 00:01:12,980
+بالمحاضرة، النظريةبداية انا عندى لازم اوفر الأدوات
+
+16
+00:01:12,980 --> 00:01:19,520
+اللازمة فى عملية السحب محتاجة string تمام ومحتاجة
+
+17
+00:01:19,520 --> 00:01:23,180
+needles هى ال needles شايفينهم تمام محتاجة alcohol
+
+18
+00:01:23,180 --> 00:01:29,440
+ومحتاجة swab وهلأ بشوف طبعا احنا أخدنا انه ال ..
+
+19
+00:01:29,440 --> 00:01:36,800
+ال ..الـ syringe مكوّنة من قطعتين، تمام؟ قطعة اللي
+
+20
+00:01:36,800 --> 00:01:41,680
+هي زي الأنبوب المدرج هذا، هاي القطعة بسميها ال
+
+21
+00:01:41,680 --> 00:01:45,260
+parallel، تمام؟ بسميها ال parallel و هاي القطعة
+
+22
+00:01:45,260 --> 00:01:50,140
+المتحركة أنا بسميها Plunger، تمام؟ بسميها Plunger،
+
+23
+00:01:50,140 --> 00:01:55,060
+إذاً ال syringe مكوّنةمن قطعتين ال parallel و ال
+
+24
+00:01:55,060 --> 00:01:58,160
+.. ال .. ال plunger هاي هم زي ما أنتوا شايفين ال
+
+25
+00:01:58,160 --> 00:02:02,580
+.. ال parallel المدرج و ال plunger اللي هو القطعة
+
+26
+00:02:02,580 --> 00:02:07,140
+المتحركة طبعا زي ما أنتوا شايفين كمان ال .. ال
+
+27
+00:02:07,140 --> 00:02:11,940
+needle مكونة من ال .. ال cap تبعها، الغطا تبعها،
+
+28
+00:02:11,940 --> 00:02:17,020
+تمام؟و أيضًا طبعًا هذا الكلاب أنا خلاص بتخلص منه
+
+29
+00:02:17,020 --> 00:02:22,500
+هي ال .. ال needle مكوّنة من عدة أدوات طبعًا إحنا
+
+30
+00:02:22,500 --> 00:02:26,640
+حكينا معلومة إنه و كذرناها كتير أثناء المحاضرة
+
+31
+00:02:26,640 --> 00:02:32,950
+النظرية حكينا إنه never everRecap bent or break
+
+32
+00:02:32,950 --> 00:02:39,390
+needle يعني أنا ممنوع أرجع أغطي ال needle بعد ما
+
+33
+00:02:39,390 --> 00:02:45,350
+أفتحها ممنوع أحاول أتنيها أو أكسرها لحد اللي اتخلص
+
+34
+00:02:45,350 --> 00:02:49,670
+منها لإنها ممكن يعني أثناء عمليةإعادة ال covering
+
+35
+00:02:49,670 --> 00:02:53,670
+مثلا ممكن أنا و أنا بحاول أرجع ال needle ل ال
+
+36
+00:02:53,670 --> 00:02:57,690
+cover تبعها أنخذ نفسي و تكون ال needle ملوثة بدم
+
+37
+00:02:57,690 --> 00:03:02,270
+المريض ساعتها أنا بكون حقنت نفسي بدم المريض تمام؟
+
+38
+00:03:02,270 --> 00:03:05,230
+هتلاحظوا أنه أنا خلال ال video ممكن أغطيها بس أنا
+
+39
+00:03:05,230 --> 00:03:08,910
+حاضرة يعني في الاستخدام ولكن أنتم هنا لأنكم لسه
+
+40
+00:03:08,910 --> 00:03:12,900
+هتكون مبتدئين و بتتعلموا ممنوع ترجعوا تغطوهاعلى
+
+41
+00:03:12,900 --> 00:03:16,440
+طول تتخلصي من ال cab و النيل بعد ما نخلص .. ساحب
+
+42
+00:03:16,440 --> 00:03:19,860
+عينة الدم، بنتخلص منها بال safety box و ان شاء
+
+43
+00:03:19,860 --> 00:03:23,760
+الله راح نرجع للمعامل و ترجعوا تشوفوا التطبيق
+
+44
+00:03:23,760 --> 00:03:27,360
+العملي كيف و راح برضه كمان احاول ان انا اشوف ..
+
+45
+00:03:27,360 --> 00:03:31,520
+يعني اسحب امامكم عينة و اورجيكم كيف انا بعمل
+
+46
+00:03:31,520 --> 00:03:36,480
+discard لكل اشي بال .. بالproper container يعني
+
+47
+00:03:36,480 --> 00:03:42,760
+مثلاال .. ال .. ال gelatin و الغطا تبع ال .. ال
+
+48
+00:03:42,760 --> 00:03:47,480
+needle و ال syringes بتخلص منه بالسلة العادية ال
+
+49
+00:03:47,480 --> 00:03:50,960
+cab مثلا ممكن بالسلة العادية ال cotton swab بعد ما
+
+50
+00:03:50,960 --> 00:03:56,160
+يتلوث بال blood مثلا إذا تلوث بال blood بتخلص منه
+
+51
+00:03:56,160 --> 00:03:59,960
+في ال bio hazard container ال syringe لإنه بيكون
+
+52
+00:03:59,960 --> 00:04:03,760
+فيها بقايا بتخلص فيهامنها في ال biohazard
+
+53
+00:04:03,760 --> 00:04:07,520
+container أيضا ال .. ال .. ال needle ال needle
+
+54
+00:04:07,520 --> 00:04:11,260
+بتخلص منها بال sharp box اللي هو الصندوق اللي
+
+55
+00:04:11,260 --> 00:04:15,900
+بتخلص من الأدوات الحاد ده فيه من خلاله تمام؟ اذا
+
+56
+00:04:15,900 --> 00:04:21,920
+.. اذا ممنوع انا ارجع اغطي هاي ال needle تمام؟ زي
+
+57
+00:04:21,920 --> 00:04:26,670
+ما انتوا شايفين ال needle مكونة من عدةأقسام القسم
+
+58
+00:04:26,670 --> 00:04:31,750
+هذا البلاستيك الملون اللي بسميه hub تمام بسميه hub
+
+59
+00:04:31,750 --> 00:04:37,510
+هذا القسم كان موجود او مدكور بالمحاضرة انه بيكون
+
+60
+00:04:37,510 --> 00:04:40,050
+عليه ال gauge number بس هنا مش موجود ال gauge
+
+61
+00:04:40,050 --> 00:04:44,110
+number ال gauge number موجود على الغلاف تبع ال
+
+62
+00:04:44,110 --> 00:04:48,850
+needle و هورجيكم إياه كمان شوية الجزء التاني من ال
+
+63
+00:04:48,850 --> 00:04:52,770
+needle اللي هو الشافت هذا الجزء المعدني اللي
+
+64
+00:04:52,770 --> 00:04:56,950
+بنسميه الشافتالجزء التالت هو الفتحة هاي المايلة
+
+65
+00:04:56,950 --> 00:05:00,570
+اللي جاية بزاوية هاي الفتحة اللي جاية بزاوية
+
+66
+00:05:00,570 --> 00:05:04,430
+بسميها ال bevel ال needle .. bevel .. فتحة ال
+
+67
+00:05:04,430 --> 00:05:08,910
+needle بسميها bevel تمام؟ مذكورة هاي الأجزاء في
+
+68
+00:05:08,910 --> 00:05:13,090
+المحاضرة النظرية و هاي انتوا هلأ شفتوها بعينيكم
+
+69
+00:05:13,090 --> 00:05:18,910
+علشان انه تحاولوا تربطوا النظري مع العملي تمام؟
+
+70
+00:05:19,760 --> 00:05:24,380
+طيب، هلأ أنا بدي أبدأ عملية سحب الدم، إيش بدي
+
+71
+00:05:24,380 --> 00:05:28,940
+أسوي؟ أول إشي بدي أحضر أدواتي، يعني هاي عندى ال
+
+72
+00:05:28,940 --> 00:05:32,340
+needle و ال syringe بدي أحضرهم، بس قبل أن أحضرهم
+
+73
+00:05:32,340 --> 00:05:35,520
+بدي أورجيكم كيف بيكون مكتوب ال gauge number، هاي
+
+74
+00:05:35,520 --> 00:05:37,760
+ال gauge number، هاي ال needle، هاي الزرقه، مكتوب
+
+75
+00:05:37,760 --> 00:05:42,800
+عليها 23 gauge، يعني رقم ال gauge number 23هي ال
+
+76
+00:05:42,800 --> 00:05:46,600
+orange ال needle اللي لونها orange ايش ال gauge
+
+77
+00:05:46,600 --> 00:05:49,220
+number اللي مكتوب عليها؟ زي ما انتوا شايفين هي هنا
+
+78
+00:05:49,220 --> 00:05:52,660
+مكتوب خمسة و عشرين جي يعني ال gauge number هادا
+
+79
+00:05:52,660 --> 00:05:57,060
+أكبر، اذا هي في القطر أصغر، تمام؟ قطرها أصغر، اذا
+
+80
+00:05:57,060 --> 00:06:01,470
+أصغر ايش ياندي انا حاليا؟ ال needle ال orangeتمام؟
+
+81
+00:06:01,470 --> 00:06:04,870
+لإنها أكبر gauge number موجود، بعدين بيجي وراها ال
+
+82
+00:06:04,870 --> 00:06:08,570
+.. ال needle الزرقى، ال needle الرمادية ال gauge
+
+83
+00:06:08,570 --> 00:06:14,410
+number 22، هنا 23، لحظوا 25، 23، 22، يعني أصغر
+
+84
+00:06:14,410 --> 00:06:21,730
+فأكبر فأكبرالقطر تمام؟ وهي ال needle الأخضر واحد
+
+85
+00:06:21,730 --> 00:06:26,870
+وعشرين gauge يعني هم مرتبين من الأصغر قطرا والأكبر
+
+86
+00:06:26,870 --> 00:06:31,190
+gauge number لحد اللي هو الأكبر قطرا والأصغر gauge
+
+87
+00:06:31,190 --> 00:06:33,810
+number تمام؟ من الأصغر gauge number للأكبر gauge
+
+88
+00:06:33,810 --> 00:06:37,850
+numbersorry من الأكبر gauge number للأصغر gauge
+
+89
+00:06:37,850 --> 00:06:41,690
+number والقطر طبعا علاقة عكسية ما بين ال gauge
+
+90
+00:06:41,690 --> 00:06:45,990
+number و ما بين ال needle هلأ هي زي ما انتوا
+
+91
+00:06:45,990 --> 00:06:51,130
+شايفين ال syringe طبعا بتكون مغلفة هستخدم هذه ال
+
+92
+00:06:51,130 --> 00:06:57,270
+..الـ needle بحيث انه اورجيكم كيف نفتحها طبعا هي
+
+93
+00:06:57,270 --> 00:06:59,830
+زي ما انتوا شايفين مغلفة بنيلون و بالجزء التاني
+
+94
+00:06:59,830 --> 00:07:05,030
+ورقي انا بفتحها من جهة الورق بهذا الشكل تمام لإنه
+
+95
+00:07:05,030 --> 00:07:08,730
+لو بدي افتحها من ناحية النيلون بدي قوة و انا لأمزع
+
+96
+00:07:08,730 --> 00:07:12,750
+النيلون فأسرع اشي من جهة الورقية هاي ال needle هنا
+
+97
+00:07:12,750 --> 00:07:15,870
+مش مستخدمة بدي اورجيكم كيف بدي افتحها من الجهة
+
+98
+00:07:15,870 --> 00:07:20,630
+اللي بدي استخدمها من جهة الورقبفتحها هيك شوي و
+
+99
+00:07:20,630 --> 00:07:24,870
+بركبهم على بعض بعدين بتخلص من الغلاف الورقي اللى
+
+100
+00:07:24,870 --> 00:07:28,590
+موجود على كل من ال syringe و ال needle بهذا الشكل
+
+101
+00:07:28,590 --> 00:07:32,750
+بتأكد من انه ال bevel فتحت ال needle مقابلة
+
+102
+00:07:32,750 --> 00:07:37,190
+للتدريج طبعا عندى هى ال syringe تلاتة سانتي بتاخد
+
+103
+00:07:37,190 --> 00:07:39,730
+فى عندى ال syringes بتاخد هيها دى ال syringe بتاخد
+
+104
+00:07:39,730 --> 00:07:42,970
+خمسة سانتي فى ال syringes بتاخد عشرة سانتي فى
+
+105
+00:07:42,970 --> 00:07:46,550
+اتنين سانتي هيها بتاخد ثلاثة سانتي متر مكعب يعني
+
+106
+00:07:46,550 --> 00:07:53,100
+تلاتة أمقالطيب و زي ما حكينا بتأكد أنه فتحت ال
+
+107
+00:07:53,100 --> 00:07:57,640
+needle اللي هنا مقابلة لإيش .. للتدريج اللي على ال
+
+108
+00:07:57,640 --> 00:08:02,580
+syringe ب .. بعد هيك بثبت ال needle بخلق .. بتأكد
+
+109
+00:08:02,580 --> 00:08:07,780
+أن هي شابقة كويس بحاول أن أنا يعني أفك ال .. ال ..
+
+110
+00:08:07,780 --> 00:08:12,930
+cap أرخي شوية و ألينالبلانجر طبعا زي ما انتوا
+
+111
+00:08:12,930 --> 00:08:17,050
+شايفين هي هنا في ال syringe هذه بدي قوة لحتى افتح
+
+112
+00:08:17,050 --> 00:08:20,850
+البلانجر لإنه البلانجر بيكون terrace طبعا ب ..
+
+113
+00:08:20,850 --> 00:08:25,390
+بقوة ب .. بافتحه فالأفضل إن أنا ألينه يعني أحركه و
+
+114
+00:08:25,390 --> 00:08:28,890
+بعدين أرجع أفرغ الهواء منه مرة تانية عشان يصير لين
+
+115
+00:08:28,890 --> 00:08:33,910
+طبعا لحتى يصير لين و سهل إن أنا أرجعه لورا أثناء
+
+116
+00:08:33,910 --> 00:08:39,550
+السحب و أبدأ أاخد ال volume اللي بديهاطبعا إذا هاي
+
+117
+00:08:39,550 --> 00:08:42,790
+ال needle و ال syringe جاهزين ال needle ثابتة
+
+118
+00:08:42,790 --> 00:08:48,110
+مركبة كويس على ال syringe و ال .. زي ما أنتوا
+
+119
+00:08:48,110 --> 00:08:53,070
+شايفين مركبة كويس ال needle على ال syringe و
+
+120
+00:08:53,070 --> 00:08:57,510
+الفتحة مقابل التدريج رخيت شوية الغطاء و لينت
+
+121
+00:08:57,510 --> 00:09:02,650
+البلنجر و طبعا إيش بلزمني بعد هيك؟ بلزمني كحول 70%
+
+122
+00:09:02,650 --> 00:09:08,670
+الكحول و بلزمني swap طبعاو هاي إيدي المريض بخليها
+
+123
+00:09:08,670 --> 00:09:13,090
+أنه ممتدة أمامي على ال bench و علشان أبدأ أسحب
+
+124
+00:09:13,090 --> 00:09:16,450
+العينة طبعا أنا بدي أسحب من منطقة ال Anticubital
+
+125
+00:09:16,450 --> 00:09:20,430
+يعني هنا في عندى median و في عندى cephalic veins
+
+126
+00:09:20,430 --> 00:09:24,130
+طبعا هذه الأوردة اللى بتكون واضحة أكتر إشي
+
+127
+00:09:24,130 --> 00:09:29,190
+للاستخدام فأنا من خلالهم بسحب عينة الدم اللى بدي
+
+128
+00:09:29,190 --> 00:09:36,350
+أسحبها تمام؟ طيب بدى .. إذا هيني أنا حضرت أدواتيال
+
+129
+00:09:36,350 --> 00:09:43,350
+syringe و ال needle و جاهزين الكحول 70% ethanol أو
+
+130
+00:09:43,350 --> 00:09:48,630
+70% isopropanol حكينا تمام بعدين طبعا ال swab بدي
+
+131
+00:09:48,630 --> 00:09:52,350
+أنا محتاجة تنتين واحدة أبللها بالكحول و تكون
+
+132
+00:09:52,350 --> 00:09:58,450
+للتعقيم و واحدة تانية جافة dry swab لحتى أحطها على
+
+133
+00:09:58,450 --> 00:10:02,650
+مكان الجرح أو مكان ال needle يعني بعد ما أخلص صاحب
+
+134
+00:10:02,650 --> 00:10:07,730
+عينة الدم تمام؟طيب بعد ما أحضر أدواتي إيش بدي
+
+135
+00:10:07,730 --> 00:10:12,030
+أعمل؟ طبعا أنا بعد ما حددت الوريد تبعي أو يعني ..
+
+136
+00:10:12,030 --> 00:10:16,910
+يعني أحددت المنطقة اللي بدي أسحب منها فبروح إيش
+
+137
+00:10:16,910 --> 00:10:22,170
+بعمل؟ بربط الternicate طبعا هاي أدواتي، هاي لسواب
+
+138
+00:10:22,170 --> 00:10:27,350
+المبلل و هاي لسواب ل dry طبعا مبلل بالكحول ال dry
+
+139
+00:10:27,350 --> 00:10:32,830
+swab علشان أحطه مكان ال .. بعد السحب و بعد هيك بدي
+
+140
+00:10:32,830 --> 00:10:39,330
+أربط الternicate تبعتيطبعا الternicate وظيفتها انه
+
+141
+00:10:39,330 --> 00:10:44,670
+انا لما بدي اربط الternicate وظيفتها انه تعملي ضغط
+
+142
+00:10:44,670 --> 00:10:49,790
+على منطقة الأوردة بحيث انها تبرزها يعني تمام؟ اذا
+
+143
+00:10:49,790 --> 00:10:53,950
+بس هي apply pressure هاي وظيفتها تمام؟ حتى تبرزلي
+
+144
+00:10:53,950 --> 00:11:00,940
+الأوردة ويسهل ساحب عينة تمام؟ال .. الternicate
+
+145
+00:11:00,940 --> 00:11:05,040
+اللي انا ممكن استخدمها يعني الرابطة ممكن استخدم ال
+
+146
+00:11:05,040 --> 00:11:08,860
+gloves العادي او ممكن في رابطة خاصة بستخدمها بتكون
+
+147
+00:11:08,860 --> 00:11:12,420
+موجودة في المختبرات اذا ما توفرت الرابطة اللي
+
+148
+00:11:12,420 --> 00:11:15,500
+موجودة في المختبرات ممكن استخدم ال gloves و عادي
+
+149
+00:11:15,500 --> 00:11:18,740
+سهل جدا ان انا استخدم ال gloves طبعا كيف انا بدي
+
+150
+00:11:18,740 --> 00:11:21,420
+.. هى طبعا هى الرابطة اللى بتكون موجودة في
+
+151
+00:11:21,420 --> 00:11:24,640
+المختبرات بحطها هنا علشان تعملى pressure طبعا
+
+152
+00:11:24,640 --> 00:11:30,920
+ببعدها بتكونهذه الرابطة أنا ببعدها عن منطقة السحب
+
+153
+00:11:30,920 --> 00:11:34,600
+بتلاتة inches يعني أنا مثلا ب .. بحط أصابعي تلاتة
+
+154
+00:11:34,600 --> 00:11:39,480
+.. تلات أصابع و بعدين بحط الرابطة بعدهم، تمام؟ فوق
+
+155
+00:11:39,480 --> 00:11:44,380
+منطقة السحب ممكن كمان في خيار تاني لو أنا مش متوفر
+
+156
+00:11:44,380 --> 00:11:48,340
+عندي هذه الرابطة ممكن أستخدم ال gloves زي ما حكينا
+
+157
+00:11:48,750 --> 00:11:52,190
+هلأ بنشوف كيف أنا بدي أربط ال gloves بالأول و
+
+158
+00:11:52,190 --> 00:11:57,970
+بعدين بنشوف كيف بنستخدم ال tourniquet التانية،
+
+159
+00:11:57,970 --> 00:12:03,490
+تمام؟ إذا حكينا إنه ال .. ال tourniquet وظيفتها
+
+160
+00:12:03,490 --> 00:12:08,270
+إنها فقط to apply pressure علشان إيش تعمليه؟ to ..
+
+161
+00:12:08,270 --> 00:12:12,090
+علشان تبرز الأوري ده و يسهل استخدام .. يسهل استحب
+
+162
+00:12:12,090 --> 00:12:16,650
+عينة الدمهلأ ال turn .. ال turniquet اللي هي ال
+
+163
+00:12:16,650 --> 00:12:19,370
+gloves أنا بمسيكها بهذا الشكل يعني من .. هي خمس
+
+164
+00:12:19,370 --> 00:12:22,470
+أصابع أنا بستخدم الأصبع .. ب .. بمسك من الأصبع
+
+165
+00:12:22,470 --> 00:12:25,370
+الوسطى و من الطرف التانى بحيث أنه تعطينى أقصى طول
+
+166
+00:12:25,370 --> 00:12:29,270
+طبعا بحيث أنه تعطينى أقصى طول و إيش بعمل؟ بربط
+
+167
+00:12:29,270 --> 00:12:32,930
+بهذا الشكل طبعا أنا هنا ماقصت تلاتة inches بس أنا
+
+168
+00:12:32,930 --> 00:12:37,110
+بدي أربط أورجيكم كيف أنا بربط بعدين ب .. بحطها على
+
+169
+00:12:37,110 --> 00:12:41,420
+المسافة اللازمة طبعاهلأ زي ما انتوا شايفين انا
+
+170
+00:12:41,420 --> 00:12:47,200
+باخدها بهذا الشكل و بعمل نص تانية تمام؟ بتنيها هيك
+
+171
+00:12:47,200 --> 00:12:53,200
+بهذا الشكل نص تانية و هيك بتكون tapped هيلاحظ ان
+
+172
+00:12:53,200 --> 00:12:58,410
+الأولي ده براليش أنا بعمل بهذا الشكل، بربط بهذا
+
+173
+00:12:58,410 --> 00:13:02,130
+الشكل؟ احنا حكينا نص فيونكا أو نص تانية ب .. بقتني
+
+174
+00:13:02,130 --> 00:13:09,090
+ال .. جانب من .. جوانب ال .. ال gloves بحيث انه لو
+
+175
+00:13:09,090 --> 00:13:12,810
+أنا خلصت سحب و بدي اجي طبعا احنا حكينا اول ما خلص
+
+176
+00:13:12,810 --> 00:13:15,910
+سحب اول اشي بعمل و برفع ال tourniquet بعدين بطلع
+
+177
+00:13:15,910 --> 00:13:21,610
+بال needle من برا لخارج ال van تمام؟ هلأ لو انا
+
+178
+00:13:23,120 --> 00:13:27,500
+ربطت هاي الربطة رح يسهل علي فكها شايفين بسهولة
+
+179
+00:13:27,500 --> 00:13:32,220
+بتفك معايا، تمام؟ لكن في حال لو أنا ربطت عقدة،
+
+180
+00:13:32,220 --> 00:13:35,540
+شوفوا كيف لو ربطت عقدة إيش بده يصير، هاي هنا أنا
+
+181
+00:13:35,540 --> 00:13:39,160
+ربطت عقدة، تمام؟ و جيت سحابة العينة و بدي أفكها
+
+182
+00:13:39,160 --> 00:13:44,660
+بصعوبة بتفك، تمام؟ بصعوبة بتفك، لكن الأفضل إنه أنا
+
+183
+00:13:44,660 --> 00:13:53,520
+أربط تمام ال .. ال .. الربطةبحيث انها تكون عندىزي
+
+184
+00:13:53,520 --> 00:13:56,580
+ما انتوا شايفين هاي جزء بكون طويل و جزء قصير بعدين
+
+185
+00:13:56,580 --> 00:14:02,060
+باخد هاي الجزء الطويل ويبتني لتحت شوي بحيث انه
+
+186
+00:14:02,060 --> 00:14:05,200
+يكون في عندي نص تانية او نص فينكة زي ما انتوا
+
+187
+00:14:05,200 --> 00:14:09,220
+شايفين هيك و هيك هي برزة الأور ده و جاهزة طبعا هنا
+
+188
+00:14:09,220 --> 00:14:13,900
+يعني ممكن يكون مش واضح قالية الربط فانا صورت فيديو
+
+189
+00:14:13,900 --> 00:14:20,280
+اخر تمام صورت فيديو بحيث انه يبينلي ال .. كيف انا
+
+190
+00:14:20,280 --> 00:14:22,820
+بربط هلأ بورجيكميا بعرضلكميا
+
+191
+00:14:25,050 --> 00:14:30,750
+تمام زى ما انتوا شايفين هى هنا هى ال gloves من
+
+192
+00:14:30,750 --> 00:14:34,250
+الإصبع الوسط بأمسكها و من الطرف التانى بحيث انها
+
+193
+00:14:34,250 --> 00:14:39,130
+بتعطينى أقصى طول و بلف بهذا الشكل على إيد المريض
+
+194
+00:14:39,130 --> 00:14:43,430
+طبعا بامسك
+
+195
+00:14:43,430 --> 00:14:51,930
+طرفينها تمامطرف طويل و طرف قصير باخد الطرف طبعا
+
+196
+00:14:51,930 --> 00:14:57,030
+يعني أنا بس عكستها على أساس يعني على هو مستوى إيدي
+
+197
+00:14:57,030 --> 00:15:03,350
+أنه انا أكون سهل علي أربطها الطرف الطويل بتنيه نص
+
+198
+00:15:03,350 --> 00:15:07,480
+تانية زي ما أنتوا شايفين هيك سحبته من هنابحيث انه
+
+199
+00:15:07,480 --> 00:15:11,140
+كان صار في عندي زي نص فينكا او نص رابطة بهذا الشكل
+
+200
+00:15:11,140 --> 00:15:17,160
+و صار في عندي اياش برزات الأوردة صار في بروز ل ال
+
+201
+00:15:17,160 --> 00:15:20,840
+.. الوريد زي ما انتوا شايفين بقى حاسس الوريد بيكون
+
+202
+00:15:20,840 --> 00:15:26,000
+شكله ملمس و مطاطي تحت ايدي تمام و هيك بفكها بكل
+
+203
+00:15:26,000 --> 00:15:30,680
+سهولة مرة تانية بربط في عندي جهة طويلة وجهة قصيرة
+
+204
+00:15:30,680 --> 00:15:33,400
+الجهة الطويلة هي اللي بتنيها تمام زي ما انتوا
+
+205
+00:15:33,400 --> 00:15:39,760
+شايفين هي بتنيها بهذا الشكلو بخليها انها مص تانية
+
+206
+00:15:39,760 --> 00:15:42,840
+او مص فيونكا يعني حسب انتوا اش فاهمين او انتوا كيف
+
+207
+00:15:42,840 --> 00:15:49,960
+ملاحظينها و بعد هيك بفكها بيبرز الوريد وسهل ان انا
+
+208
+00:15:49,960 --> 00:15:55,640
+افكها او اتخلص منها فالنهاية عملية السحب تمام؟ اذا
+
+209
+00:15:55,640 --> 00:16:05,920
+هذا هو ال .. كيف يمكن ربط ال .. الفيونكا تمام؟
+
+210
+00:16:08,660 --> 00:16:15,500
+بس نرجع تانى لحد ما كنا تمام
+
+211
+00:16:15,500 --> 00:16:27,520
+تمام
+
+212
+00:16:27,520 --> 00:16:32,580
+هيك ربطت تمام
+
+213
+00:16:32,580 --> 00:16:36,620
+بس هذه إعادة لعملية الربط
+
+214
+00:16:46,150 --> 00:16:50,690
+زي ما أنتوا شايفين نص .. ربطة نص فيونكا حسب أنتوا
+
+215
+00:16:50,690 --> 00:16:57,490
+إيش تسموها و ال .. قبل ال .. الأوردة بتبرز لإن دور
+
+216
+00:16:57,490 --> 00:17:03,030
+الternicate أو الربطة إنها تعملي ضغط فقط ضغططبعا،
+
+217
+00:17:03,030 --> 00:17:07,090
+النوع الآخر من الternicates زي ما أنتوا شايفين أنا
+
+218
+00:17:07,090 --> 00:17:10,890
+فيه عندي ternicate بتكون عبارة عن حزام، طبعا، فهذا
+
+219
+00:17:10,890 --> 00:17:15,590
+الحزام بس ما علي إنه غير أركبه يعني و أشده لحتى
+
+220
+00:17:15,590 --> 00:17:20,310
+يعطيني ضغط، اذا أنا بس علي أكبس الكبسة تبعته و
+
+221
+00:17:20,310 --> 00:17:26,600
+أشده لحتى يعملي ضغط، طبعابهذا الشكل طبعا فقط لا
+
+222
+00:17:26,600 --> 00:17:31,440
+غير بهذا الشكل انا بعمله اذا يا اما الرابطة هاي او
+
+223
+00:17:31,440 --> 00:17:36,060
+ال gloves سيان المهم اذا ما اتوفر عندي هاي الرابطة
+
+224
+00:17:36,060 --> 00:17:40,220
+انا بستخدم ال gloves طبعا احنا حكينا انه لازم تكون
+
+225
+00:17:40,220 --> 00:17:43,360
+الرابطة على بعد تلاتة inches يعني انا بجيس تلات
+
+226
+00:17:43,360 --> 00:17:46,400
+أصابع فوق منطقة الوريد اللي انا بده أسحب منها و
+
+227
+00:17:46,400 --> 00:17:49,100
+بعدين باربط الرابطة طبعا
+
+228
+00:17:52,190 --> 00:18:01,310
+طبعا بركبها على بعض تلاتة inches و بسكر بضغطها
+
+229
+00:18:01,310 --> 00:18:11,170
+بشدها و بصير عندى الوريد بارز و جاهز لعملية السحب
+
+230
+00:18:11,320 --> 00:18:15,980
+طبعا طيب إذا أنا ربطت ال .. ال tourniquet و لمست
+
+231
+00:18:15,980 --> 00:18:19,640
+الوريد حددته طبعا بسأل المريض بخلي المريض أنه يعمل
+
+232
+00:18:19,640 --> 00:18:23,620
+قبضة بإيده ما يظل يفتح و يسكر لأ بس قبضه و يغلقها
+
+233
+00:18:23,620 --> 00:18:27,580
+و حددت الوريد اللي أنا بدي أسحبه بعد ما لمسته و
+
+234
+00:18:27,580 --> 00:18:31,900
+اتحسسته طبعا و حكينا أنه مالمسه مطاطي بحس أنه في
+
+235
+00:18:31,900 --> 00:18:36,650
+إشي مطاطي تحت إيديمع الممارسة راح تعرفوا تمام؟ كيف
+
+236
+00:18:36,650 --> 00:18:40,690
+عملية التعقيم بال .. بالسواب اللي مبللة بالكحول
+
+237
+00:18:40,690 --> 00:18:45,710
+بعمل بشكل دائري ببدأ من دائر .. بدا .. من يعني ضيق
+
+238
+00:18:45,710 --> 00:18:50,270
+و ببدأ أوسع في الدائرة زي ما أنتوا شايفين ليش طيب
+
+239
+00:18:50,270 --> 00:18:52,990
+أنا بعمل هيك علشان أنا باخد ال contamination من
+
+240
+00:18:52,990 --> 00:18:56,710
+منطقة يعني أي contamination موجودة في منطقة الساحب
+
+241
+00:18:56,710 --> 00:19:01,090
+باخدها و ببعدها على ال .. الأطرافتمام؟ لو أنا بدي
+
+242
+00:19:01,090 --> 00:19:04,550
+أعمل العكس، تمام؟ لو بدي أعمل العكس، يعني بدي ألم
+
+243
+00:19:04,550 --> 00:19:07,890
+و أجي أ .. أركز و كله في المنتصف، يعني أنا هيك
+
+244
+00:19:07,890 --> 00:19:10,950
+لميت ال contamination كلها و حطيتها في منطقة
+
+245
+00:19:10,950 --> 00:19:16,360
+المنتصف، و هذا إشي غلط، إذا ال .. ال ..يعني ببدأ
+
+246
+00:19:16,360 --> 00:19:20,380
+ضايق و ببدأ أوسع الدائرة هذا هو الطريقة الصحيحة
+
+247
+00:19:20,380 --> 00:19:23,960
+لإتعاقيم أو ممكن مسحة زي ما انتوا شايفين هيك مسحة
+
+248
+00:19:23,960 --> 00:19:27,780
+واحدة فقط و لو كان في عندي excess alcohol بنشفه
+
+249
+00:19:27,780 --> 00:19:33,920
+بسوابجاف لإنه ال alcohol حكينا إنه ممكن يعملي
+
+250
+00:19:33,920 --> 00:19:38,900
+مشاكل hemoconcentration أو hemolysis أو يعني يأثر
+
+251
+00:19:38,900 --> 00:19:44,340
+فبالتالي حكينا إنه بأتخلص منه، هذا هو الوريد اللي
+
+252
+00:19:44,340 --> 00:19:48,300
+أنا بدي أسحب منه، حكينا إنه ما .. يعني ما بص ..
+
+253
+00:19:48,300 --> 00:19:53,060
+بيصير ألمس الوريد بعد التعقيمطبعا أنا بس هنا جاية
+
+254
+00:19:53,060 --> 00:19:55,680
+بحدد .. بس أحكيلكم أن هذا هو الوريد اللي بدي أسحب
+
+255
+00:19:55,680 --> 00:20:00,160
+منه و هرجع أعقب مرة تانية أنا لازم أكون لمسته و
+
+256
+00:20:00,160 --> 00:20:05,820
+حددته قبل عملية التعقيم طبعا إذا بعقم مرة تانية و
+
+257
+00:20:05,820 --> 00:20:11,800
+بجفف ال excess alcohol اللي موجود و بعد هيك باجي
+
+258
+00:20:11,800 --> 00:20:16,740
+بال syringe و ال needle بفتحها ب .. بمسك لل
+
+259
+00:20:16,740 --> 00:20:23,790
+syringe و ال needle مسكة خاصة بحيث أنهال .. زي ما
+
+260
+00:20:23,790 --> 00:20:28,270
+انتوا شايفين ال .. التلات أصابع تبعوني تحت ال ..
+
+261
+00:20:28,270 --> 00:20:31,650
+ال .. التلات أصابع هدول تحت ال syringe تمام؟
+
+262
+00:20:31,650 --> 00:20:34,790
+الإبهام فوق ال syringe و السبابة عند منطقة ال hub
+
+263
+00:20:34,790 --> 00:20:39,050
+زي ما انتوا شايفين فتحت ال needle مقابلة للتدريج و
+
+264
+00:20:39,050 --> 00:20:42,830
+ممكن أنا أشد ال skin بالإبهام أو ممكن أتركها أنا
+
+265
+00:20:42,830 --> 00:20:45,630
+في هذه الحالة الوريد واضح و مش محتاجة أشد ال ..
+
+266
+00:20:45,630 --> 00:20:49,870
+أشد ال skin بال .. بإصبعي التاني الإبهامفإذا بمسك
+
+267
+00:20:49,870 --> 00:20:53,810
+و زي ما انتوا شايفين هذا هو اتجاه الوريد انا بفوت
+
+268
+00:20:53,810 --> 00:20:58,670
+في نفس اتجاه الوريد تمام؟ مابنفع انا اجي هيك بهذا
+
+269
+00:20:58,670 --> 00:21:04,050
+الشكل افوت لأ بفوت في نفس اتجاه الوريد حكينا لإنه
+
+270
+00:21:04,050 --> 00:21:07,430
+لو انا بدي افوت مثلا في اتجاه معاكس ممكن انا اخترق
+
+271
+00:21:07,430 --> 00:21:11,330
+الوريد من الجهة التانية و يبدأ يتراكم الدم تحت ال
+
+272
+00:21:11,330 --> 00:21:14,850
+skin و يعمللي hematoma و ممكن يتوقف عند السحب في
+
+273
+00:21:14,850 --> 00:21:20,660
+داخل ال syringeفإذا أنا باجي في نفس اتجاه الوريد و
+
+274
+00:21:20,660 --> 00:21:25,840
+بفوت طبعا بفوت في زاوية معينة اللي طبعا إذا كانت
+
+275
+00:21:25,840 --> 00:21:28,840
+الوريد superficial سطحي زي ما أنتوا شايفين هنا
+
+276
+00:21:28,840 --> 00:21:33,600
+واضح ب .. بتكون الزاوية قليلة إذا كان ال .. الوريد
+
+277
+00:21:33,600 --> 00:21:39,230
+أنا حاساه بس مش شايفاه يعني هو ديب تمام؟بكبر
+
+278
+00:21:39,230 --> 00:21:42,510
+الزاوية و هذه أشياء ان شاء الله رح تتعلموها مع
+
+279
+00:21:42,510 --> 00:21:46,890
+الممارسة بذكرها الآن ولكن رح تتعلموها مع الممارسة
+
+280
+00:21:46,890 --> 00:21:51,890
+و زي ما انتوا شايفين بفوت في .. طبعا بكون طبعا زي
+
+281
+00:21:51,890 --> 00:21:56,680
+ما انتوا شايفين هنا إيديعلى ال skin تبعت المريض
+
+282
+00:21:56,680 --> 00:22:00,640
+بثبت إيدي على ال skin إيدي هاي التلت أصابع اللي هم
+
+283
+00:22:00,640 --> 00:22:04,800
+تحت ال syringe بثبتهم على إيدي المريض عشان لما أجي
+
+284
+00:22:04,800 --> 00:22:11,060
+أسحب البلنجر تبع ال syringe تكون إيدي ثابتة مارجعش
+
+285
+00:22:12,160 --> 00:22:16,360
+لاتلقائي و يعني لاشعوريا بحس حالي برجع و بطلع من
+
+286
+00:22:16,360 --> 00:22:20,380
+الواريد بدون ما اسحب الكمية اللازمة لأ فإذا بثبت
+
+287
+00:22:20,380 --> 00:22:24,140
+زي ما أنتوا شايفين بثبت إيدي على .. على إيد المريض
+
+288
+00:22:24,140 --> 00:22:30,460
+و بفوت في نفس اتجاهالوريد تمام بزاوية قليلة لإن هو
+
+289
+00:22:30,460 --> 00:22:35,780
+superficial و بعد هيك أول ما بفوت في الوريد الصح
+
+290
+00:22:35,780 --> 00:22:39,700
+بتعلم عندي زي ما انتوا شايفين هنا بتعلم عندي نقطة
+
+291
+00:22:39,700 --> 00:22:43,380
+blood في منطقة ال hub يعني معناها إنه خلاص المسار
+
+292
+00:22:43,380 --> 00:22:50,750
+الدم فاتح ببدأ إيش أعمل؟برجع البلانجر لورا طبعا
+
+293
+00:22:50,750 --> 00:22:54,170
+اذا هيتكونت عندى نقطة دم برجع البلانجر لورا زى ما
+
+294
+00:22:54,170 --> 00:23:00,890
+انتوا شايفين ويبدأ يعبي blood داخل ال syringe طبعا
+
+295
+00:23:00,890 --> 00:23:04,990
+هيتعبى انا هنا باخد ال volume اللى بديه طبعا حسب
+
+296
+00:23:04,990 --> 00:23:08,830
+ال volume اللى بديه انا محتاجة اليوم اخد بس 1 cm
+
+297
+00:23:08,830 --> 00:23:12,850
+لإن هذا فيديو تعليمى بس بوضحلكم قالية السحب فقط لا
+
+298
+00:23:12,850 --> 00:23:19,060
+غيرولكن إذا كان يعني أنا محتاجة volume أكتر باخد
+
+299
+00:23:19,060 --> 00:23:23,280
+volume أكتر بعدين طبعا زي ما أنتوا شايفين بعد ما
+
+300
+00:23:23,280 --> 00:23:26,680
+أخد ال volume اللي بديا أول خطوة و أنا لساتني جوا
+
+301
+00:23:26,680 --> 00:23:33,290
+إيش بعمل؟ بفك الternicate طبعاو بعدين هيخلصت فكات
+
+302
+00:23:33,290 --> 00:23:38,310
+ال turnicate بحط ال dry swab على منطقة السحب بطلع
+
+303
+00:23:38,310 --> 00:23:41,770
+بدون ما اضغط بعدين بخليه يضغط بحكي للمريض اضغط ف
+
+304
+00:23:41,770 --> 00:23:45,910
+.. و بخليه بتأكد ان هو عندى يضغط هكذا انا أخدت ال
+
+305
+00:23:45,910 --> 00:23:52,340
+volume اللى انا بدياه تمام؟و بعد هيك بفضيه في ال
+
+306
+00:23:52,340 --> 00:23:56,440
+tube سواء كان ال tube هذه فيها edita او heparin او
+
+307
+00:23:56,440 --> 00:24:00,840
+sodium citrate او كانت فاضية مش فيها اشي اي نوع ال
+
+308
+00:24:00,840 --> 00:24:04,560
+tube طبعا حسب نوع الفحص اللي انا بدي اعمله بكون
+
+309
+00:24:04,560 --> 00:24:07,720
+عارفة الفحص اللي بدي اعمله و عارفة ال tube اللي
+
+310
+00:24:07,720 --> 00:24:12,040
+بدي استخدمها و ايش فيها additive ايش نوع ال
+
+311
+00:24:12,040 --> 00:24:15,910
+anticoagulant اللي فيهاتمام؟ وإذا كان أصلا فيها
+
+312
+00:24:15,910 --> 00:24:19,950
+anticoagulant أو لا ممكن أكون محتاجة أنه أنا بدي
+
+313
+00:24:19,950 --> 00:24:24,970
+serum فأحطها في tube إما إبلان فاضية مش فيها إشي
+
+314
+00:24:24,970 --> 00:24:30,570
+أو tube مثلا فيها clot activator أو clot activator
+
+315
+00:24:30,570 --> 00:24:33,490
+مع جل أو أي إشي من الجدول اللي إحنا ذكرناها في
+
+316
+00:24:33,490 --> 00:24:39,850
+محاضرة ال blood component تمام؟ طيبالمحتويات ال
+
+317
+00:24:39,850 --> 00:24:43,690
+syringe اللى عينة الدم انا بفضيها immediately حالا
+
+318
+00:24:43,690 --> 00:24:45,890
+يعنى مابستنى عليها مابحطها على ال bench و اروح
+
+319
+00:24:45,890 --> 00:24:51,390
+مثلا اعمل شغل بعدين ارجع افرغها داخل ال tube لإنه
+
+320
+00:24:51,390 --> 00:24:54,600
+هنا ال blood راح يتجلط تمام؟راح يصير في
+
+321
+00:24:54,600 --> 00:24:59,380
+coagulation فبالتالي لازم انا افرغها مباشرة او
+
+322
+00:24:59,380 --> 00:25:03,300
+immediately في الحال في داخل ال tube طبعا انا
+
+323
+00:25:03,300 --> 00:25:10,920
+اليوم انا جايبالكم مثال ال tube يعني هو مافيش فيه
+
+324
+00:25:10,920 --> 00:25:15,620
+اشي بس فيه clot activator طبعا؟ لذا انا بفضل محتوى
+
+325
+00:25:15,620 --> 00:25:20,660
+ال blood في ال tube الخاصة و طبعا immediately فورا
+
+326
+00:25:20,660 --> 00:25:24,910
+هيهانا في عندنا مثلا ال tubeزي ما انتوا شايفين
+
+327
+00:25:24,910 --> 00:25:31,110
+نعمل هايو Red color نعمل focus بس على منطقة ال
+
+328
+00:25:31,110 --> 00:25:36,030
+labeling زي ما انتوا شايفين هنا مكتوب ان في Clot
+
+329
+00:25:36,030 --> 00:25:39,310
+activator هاي الزهادي اللي انا حكيتلكم ال additive
+
+330
+00:25:39,310 --> 00:25:41,730
+انتوا ما بتشوفوها يعني ال anticoagulant داخل ال
+
+331
+00:25:41,730 --> 00:25:44,950
+tube ممكن يكون عبارة عن قطارات ممكن يكون عبارة عن
+
+332
+00:25:44,950 --> 00:25:47,430
+powder موجودة على الجدار يعني انتوا ما بتشوفوه زي
+
+333
+00:25:47,430 --> 00:25:52,730
+ما شايفين هاي ال .. ال ..الأشياء اللي موجودة داخل
+
+334
+00:25:52,730 --> 00:25:57,330
+ال tube اللي عاملة زي تمشيحة هذه اللي احنا بنحكي
+
+335
+00:25:57,330 --> 00:26:01,350
+عنها clot activator تمام انا بحط ال blood فيه فهذا
+
+336
+00:26:01,350 --> 00:26:05,950
+بيشجع انه يصير في عندي عملية coagulation تجلط للدم
+
+337
+00:26:05,950 --> 00:26:11,920
+فإذا لما انا بدي افصل العينة هذه اللي بيفصل فوقال
+
+338
+00:26:11,920 --> 00:26:16,280
+blood فوق ال clot لو انا بدي ااخد طبعا السائل اللى
+
+339
+00:26:16,280 --> 00:26:19,540
+بيكون موجود فوق ال blood هذا السائل بيكون عبارة عن
+
+340
+00:26:19,540 --> 00:26:22,200
+serum طبعا بيكون عبارة عن serum يعني مافيش فيه
+
+341
+00:26:22,200 --> 00:26:26,700
+fibrin طبعا هذه بنات عبارة عن vacotainery tube
+
+342
+00:26:26,700 --> 00:26:32,560
+يعني انا بحطها في جهة .. في الادات ال vacotainer و
+
+343
+00:26:32,560 --> 00:26:35,560
+ببدأ أسحب على طول مباشرة بيعبئ دم منها من خلال
+
+344
+00:26:35,560 --> 00:26:39,840
+الوريدمش بتوفر عندى vacutainer ف .. ولكن هورجيكميا
+
+345
+00:26:39,840 --> 00:26:43,900
+ان شاء الله خلال عودتنا ل .. المعابل ان شاء الله
+
+346
+00:26:43,900 --> 00:26:48,500
+إذا رجعنا بالسلامة و راح تشوفوا راح أسحبلكم
+
+347
+00:26:48,500 --> 00:26:51,180
+باستخدام vacutainer لحتى تشوفوا كيف انه أنا
+
+348
+00:26:51,180 --> 00:26:57,200
+باستخدام هاي القنابيب على طول بتعبي فيها الدم
+
+349
+00:26:59,190 --> 00:27:01,750
+زي ما انتوا شايفين هنا ال .. ال cover عبارة عن
+
+350
+00:27:01,750 --> 00:27:07,890
+مطاط و .. يعني سهل انه يخت .. تخترقوا ال needle و
+
+351
+00:27:07,890 --> 00:27:12,310
+يتم تعبئة ال blood داخل ال tube طبعا؟ اللي انا لو
+
+352
+00:27:12,310 --> 00:27:17,170
+بدي افرغ ال blood داخل ال tube هاي في عندي طريقتين
+
+353
+00:27:17,170 --> 00:27:21,770
+اما انه انا افتح ال tube يعني اشيل الغطا و افض على
+
+354
+00:27:21,770 --> 00:27:26,870
+الجدار طبعا؟و أتخلص من ال needle بعدين أفضّي
+
+355
+00:27:26,870 --> 00:27:30,270
+محتويات ال syringe على الجدار بحيث انه ما يتكسر
+
+356
+00:27:30,270 --> 00:27:33,750
+الدم بعدين أرجع أغطي ال tube أعمل mix و أعمل
+
+357
+00:27:33,750 --> 00:27:37,790
+labeling أكتب ال label معلومات المريض في المكان
+
+358
+00:27:37,790 --> 00:27:44,610
+المخصص هنا أو الممكن بطريقة تانية انهإنه باستخدام
+
+359
+00:27:44,610 --> 00:27:48,450
+ال needle أخترق المرتقة المطاطية و يهادي الأنبوب
+
+360
+00:27:48,450 --> 00:27:52,070
+مفرغة من الهواء فعلي جد ما هي مفرغة من الهواء راح
+
+361
+00:27:52,070 --> 00:27:56,030
+تسحب volume راح تسحب سائل فمجرد ما أنا أخترق ال
+
+362
+00:27:56,030 --> 00:28:01,770
+needle أخترق الغطا بال needle راح على طول يعبي ال
+
+363
+00:28:01,770 --> 00:28:05,810
+blood داخل ال tube تمام؟ إذا هذه ال tube مبسطة
+
+364
+00:28:05,810 --> 00:28:09,530
+عليا إن أنا ممكن أعبيها إما على الجدار أو باستخدام
+
+365
+00:28:09,530 --> 00:28:15,940
+ال ..ال .. يعني أنه أنا أخترق ال .. الغطاء المطاطي
+
+366
+00:28:15,940 --> 00:28:18,280
+اللي موجود فيها وهي الغطاء المطاطي اللي هذا اللي
+
+367
+00:28:18,280 --> 00:28:22,260
+لونه رمادي سهل اختراقه بال needle تمام؟ زي ال ..
+
+368
+00:28:22,260 --> 00:28:29,470
+يعني اشي جلديس .. مطاطي تمام؟هذا بالنسبة لل
+
+369
+00:28:29,470 --> 00:28:34,010
+YouTube ضروري جدا بعد ما أنا أعبي العينة أفضيها
+
+370
+00:28:34,010 --> 00:28:41,050
+أعمل mix و أعمل labeling أحط معلومات المريض اسمه
+
+371
+00:28:41,050 --> 00:28:46,790
+ال initial و ال surname و ال middle initial و أحط
+
+372
+00:28:46,790 --> 00:28:50,430
+تاريخ و وقت السحب و ال initial of ال optimist على
+
+373
+00:28:50,430 --> 00:28:57,060
+العينة تمام؟و هيك بتكون عندى صارت العينة جاهزة
+
+374
+00:28:57,060 --> 00:28:59,800
+للفخصات ممكن انا احطها جهة .. داخل جهاز ال
+
+375
+00:28:59,800 --> 00:29:07,840
+centrifuge و افصل تعطينى طبعا serum طبعا تعطينى
+
+376
+00:29:07,840 --> 00:29:12,280
+serum طبعا هيك احنا بنكون اتعلمنا كيف انا اسحب
+
+377
+00:29:12,280 --> 00:29:17,900
+عينة من الوريد تمام ان شاء الله فور توفر ال ..
+
+378
+00:29:17,900 --> 00:29:23,240
+الأدواتالخاصة بسحب عينة من ال skin سواء skin
+
+379
+00:29:23,240 --> 00:29:27,140
+puncture يعني سواء من الإصبع أو من ال heel راح ان
+
+380
+00:29:27,140 --> 00:29:31,200
+شاء الله راح أشرحلكم إياها في فيديو تعليم آخر و ان
+
+381
+00:29:31,200 --> 00:29:34,440
+شاء الله تكونوا هيك استفدتوا و الأشياء يعني أو
+
+382
+00:29:34,440 --> 00:29:37,110
+المعلومات اللي احنا ذكرناها كيف أنا أفوت فيهافي
+
+383
+00:29:37,110 --> 00:29:40,870
+اتجاه الوريد، كيف اعقم، كيف اربط الternicate، هذه
+
+384
+00:29:40,870 --> 00:29:44,290
+كلها الشغلات ان شاء الله تكون واضحة وشوفتوها
+
+385
+00:29:44,290 --> 00:29:50,690
+بعينيكم و سهر ممكن انتوا تتخيلوها حاولوا انه توفري
+
+386
+00:29:50,690 --> 00:29:55,550
+gloves و تحاولوا تتعلمي كيف تربطي الternicate مثلا
+
+387
+00:29:55,550 --> 00:30:00,090
+على ايد أختك او ايد احد من أسرتك كمان يعني انه
+
+388
+00:30:00,090 --> 00:30:04,290
+لحتى ايدك تاخد على الرابطةتمام لحتى إيدك تاخد على
+
+389
+00:30:04,290 --> 00:30:07,650
+الربطة كرريها مرتين تلاتة شوفي حالك كده ممكن أنت
+
+390
+00:30:07,650 --> 00:30:11,530
+تعقمي بشكل دائي بس ماتجربي عملية السحب إلا لما
+
+391
+00:30:11,530 --> 00:30:15,530
+اتجربيها عندى بالمختبر بعدين بخليكي انه .. انه
+
+392
+00:30:15,530 --> 00:30:21,740
+مثلا اتجربيها على متطوعين او يعني whateverتمام؟
+
+393
+00:30:21,740 --> 00:30:26,960
+إذا هك بنكون خلصنا جزئية ال pheloptomy اللي هو سحب
+
+394
+00:30:26,960 --> 00:30:34,160
+عين الدم من الوريد تمام؟ هذا ال .. الجزء .. أو هذا
+
+395
+00:30:34,160 --> 00:30:36,500
+ال .. الفيديو التعليمي اللي أنا سجلتلكم إياه ان
+
+396
+00:30:36,500 --> 00:30:42,000
+شاء الله يكون .. تكونوا استفدته من خلاله و يعطيكم
+
+397
+00:30:42,000 --> 00:30:46,800
+العافية ان شاء الله بنسجللكم فيديوهات أخرى بتوضح
+
+398
+00:30:46,800 --> 00:30:48,700
+عملية ال skin puncture
+