diff --git a/PL9fwy3NUQKwZWgAb_V1ATlRRsH-6i3cPC/-CoHtax7Ikg.srt b/PL9fwy3NUQKwZWgAb_V1ATlRRsH-6i3cPC/-CoHtax7Ikg.srt
new file mode 100644
index 0000000000000000000000000000000000000000..96c70804587d6c4ba7262b9c22275eb9ae6ccf79
--- /dev/null
+++ b/PL9fwy3NUQKwZWgAb_V1ATlRRsH-6i3cPC/-CoHtax7Ikg.srt
@@ -0,0 +1,11 @@
+1
+00:00:19,710 --> 00:00:23,810
+السلام عليكم ورحمة الله وبركاته، نستقبل معكم سلسلة
+
+2
+00:00:23,810 --> 00:00:27,990
+Microbiologia،  الجامعة الإسلامية. فيديو اليوم بأنواع
+
+3
+00:00:27,990 --> 00:00:29,170
+كبسول الثاني
diff --git a/PL9fwy3NUQKwZWgAb_V1ATlRRsH-6i3cPC/4-SUdsxRvPI.srt b/PL9fwy3NUQKwZWgAb_V1ATlRRsH-6i3cPC/4-SUdsxRvPI.srt
new file mode 100644
index 0000000000000000000000000000000000000000..fd4b99cc192d635458b8c6e2c847b8d17349c66c
--- /dev/null
+++ b/PL9fwy3NUQKwZWgAb_V1ATlRRsH-6i3cPC/4-SUdsxRvPI.srt
@@ -0,0 +1,1090 @@
+1
+00:00:01,480 --> 00:00:05,740
+Acid-Fast Stain أو الإسم الآخر Ziehl-Neelsen Stain
+
+2
+00:00:05,740 --> 00:00:10,360
+علمان اكتشفوا هذه الصبغة فسميت باسمها The Acid
+
+3
+00:00:10,360 --> 00:00:13,180
+Fast Stain is another differential staining method
+
+4
+00:00:13,180 --> 00:00:15,900
+اتفقنا إنه هي نوع من أنواع الـDifferential Stain
+
+5
+00:00:15,900 --> 00:00:19,900
+اللي هتقسم البكتيريا لمجموعة، فهتقسم البكتيريا ل
+
+6
+00:00:19,900 --> 00:00:24,640
+Acid-Fast  و Non Acid-Fast
+
+7
+00:00:24,640 --> 00:00:27,540
+في الإجراء الستان، قلنا إنه مش كل البكتيريا بقدر
+
+8
+00:00:27,540 --> 00:00:30,770
+أصبغها، في عندي استثناء، في عندي بكتيريا الـ
+
+9
+00:00:30,770 --> 00:00:34,750
+*Mycobacterium*، هذه البكتيريا ما بقدر أصبغها بالـ
+
+10
+00:00:34,750 --> 00:00:38,610
+Gram Stain، عشان هيك بستخدم الـ Acid-Fast Stain
+
+11
+00:00:38,610 --> 00:00:43,210
+لصباغتها، in this case, the target cells are usually
+
+12
+00:00:43,210 --> 00:00:46,310
+members of the genus *Mycobacterium*.
+
+13
+00:00:46,310 --> 00:00:49,430
+*Mycobacterium* هي اللي بستهدفها في الـ Acid-Fast
+
+14
+00:00:49,430 --> 00:00:53,430
+Stain. طيب ليش أنا ما أقدر أصبغ الـ *Mycobacterium* بالـ
+
+15
+00:00:53,430 --> 00:00:57,380
+Gram Stain أو حتى بالـSimple Stain؟ بقول لي لأن هاي
+
+16
+00:00:57,380 --> 00:01:00,760
+البكتيريا، الـ cell wall تبعها فيه high concentration
+
+17
+00:01:00,760 --> 00:01:04,680
+of mycolic acid.  الـ mycolic acid هاي بتكون كحاجز
+
+18
+00:01:04,680 --> 00:01:09,280
+بتمنع دخول الصبغة داخل البكتيريا، وحتى لو أنا قدرت
+
+19
+00:01:09,280 --> 00:01:13,440
+أصبغها، صعب أني أنا أزيلها. the cell walls of
+
+20
+00:01:13,440 --> 00:01:16,740
+these bacteria contain an unusually high
+
+21
+00:01:16,740 --> 00:01:19,660
+concentration of mycolic acid. The staining
+
+22
+00:01:19,660 --> 00:01:22,440
+conventional Simple stain and the Gram stain
+
+23
+00:01:22,440 --> 00:01:27,190
+are useless. هذا بيجعل صباغتها صعبة بالـ Gram Stain
+
+24
+00:01:27,190 --> 00:01:31,190
+والـSimple Stain. The genus *Mycobacterium*, الـ
+
+25
+00:01:31,190 --> 00:01:35,170
+*Mycobacterium* هو يعتبر genus containing two important
+
+26
+00:01:35,170 --> 00:01:39,830
+human pathogens. في عندي في هذه العائلة two species
+
+27
+00:01:39,830 --> 00:01:44,010
+مهمين بيسببا مرض للإنسان، الـ *Mycobacterium*
+
+28
+00:01:44,010 --> 00:01:47,870
+tuberculosis اللي بيسبب السل الرئوي، والـ
+
+29
+00:01:47,870 --> 00:01:52,430
+*Mycobacterium leprae* هاي اللي بتسبب الجذام، مرض
+
+30
+00:01:52,430 --> 00:01:57,170
+بيكون فيه ندبات بالجلد (skin scars) وبيصير فيه تدمير
+
+31
+00:01:57,170 --> 00:02:02,750
+بالجهاز العصبي (brain damage). الـ *M* رمز لجيناس
+
+32
+00:02:02,750 --> 00:02:05,770
+*Mycobacterium*.  الـ *tuberculosis* هي الـ species
+
+33
+00:02:05,770 --> 00:02:10,570
+الكتابة العلمية للبكتيريا لازم أني أحط خط أو
+
+34
+00:02:10,570 --> 00:02:18,070
+أنها تكون الكتابة مائلة. هأبدأ
+
+35
+00:02:18,070 --> 00:02:22,600
+بشرح الـ procedure لأن من خلالها هنتعرف كيف بدي أقدر
+
+36
+00:02:22,600 --> 00:02:27,420
+أصبغ هاي البكتيريا اللي صعب صباغتها بأي Stain في
+
+37
+00:02:27,420 --> 00:02:32,000
+معمل الـ Microbiology. قولنا خطوات الصباغة: Smear
+
+38
+00:02:32,000 --> 00:02:35,960
+preparation، Air-drying، Heat-fixation، والـ Stain،
+
+39
+00:02:35,960 --> 00:02:40,780
+الـ Stain بتختلف حسب نوع الصباغة. هنتكلم على الـ Acid
+
+40
+00:02:40,780 --> 00:02:44,140
+-fast stain. في الـ Gram stain، الـ primary stain
+
+41
+00:02:44,140 --> 00:02:47,600
+كانت Crystal Violet، لكن في الـ Acid-fast stain
+
+42
+00:02:47,600 --> 00:02:52,220
+هتختلف اسمها وهتختلف طريقة صباغتها. الـ primary stain
+
+43
+00:02:52,220 --> 00:02:56,180
+في الـ Acid-Fast Stain هو Carbol fuchsin. الـ Carbol fuchsin
+
+44
+00:02:56,180 --> 00:02:59,820
+إذا متذكرين في معمل الـ Simple stain، قلنا هي Basic
+
+45
+00:02:59,820 --> 00:03:04,460
+stain، لكن اللي بيميز الـ Carbol fuchsin إنها Lipid
+
+46
+00:03:04,460 --> 00:03:07,800
+-soluble، والتي إحنا قلنا إنه الـ *Mycobacterium*
+
+47
+00:03:07,800 --> 00:03:11,340
+بتحتوي على كمية كبيرة من الدهون، عشان هيك ما أقدر
+
+48
+00:03:11,340 --> 00:03:15,520
+أصبغها بالـSimple stain أو بالـGram stain. من خلال هذه
+
+49
+00:03:15,520 --> 00:03:21,440
+الخاصية، هتساعد على اختراق الصبغة للـ waxy lipids، لكن
+
+50
+00:03:21,440 --> 00:03:24,880
+بقولنا إن الشيء الرئيسي اللي هيساعد على اختراق الـ
+
+51
+00:03:24,880 --> 00:03:29,980
+Carbol fuchsin للـ waxy lipids هو الـ Steam. شو يعني
+
+52
+00:03:29,980 --> 00:03:34,620
+Steam؟ الـ Steam هو البخار.  تعريض الصبغة للبخار، ليش
+
+53
+00:03:34,620 --> 00:03:38,120
+تعريض الصبغة للبخار؟ بقول لي البخار هيعمل الـ
+
+54
+00:03:38,120 --> 00:03:42,240
+denaturation للروابط الكربونية الموجودة في الـ
+
+55
+00:03:42,240 --> 00:03:46,580
+mycolic acid اللي هو الـ waxy lipids، بالتالي هيساعد
+
+56
+00:03:46,580 --> 00:03:52,100
+على اختراق الصبغة للـ waxy lipids. هيك أنا قدرت أصبغ
+
+57
+00:03:52,100 --> 00:03:55,340
+هذه البكتيريا من خلال استخدام الـ primary stain تاني،
+
+58
+00:03:55,340 --> 00:03:59,360
+الـCarbol fuchsin اللي هي Lipid-soluble. الشيء الأساسي
+
+59
+00:03:59,360 --> 00:04:05,000
+من خلاله قدرت أصبغ، اللي هو تعريض البكتيريا لـ
+
+60
+00:04:05,000 --> 00:04:08,720
+Steam (بخار). هيكسر الروابط الكربونية الموجودة في
+
+61
+00:04:08,720 --> 00:04:12,960
+الـ mycolic acid، بالتالي هيساعد على اختراق الصبغة
+
+62
+00:04:12,960 --> 00:04:17,500
+للـ mycolic acid. كيف أنا بدي أعمل هذه الخطوة؟ بروح
+
+63
+00:04:17,500 --> 00:04:21,610
+بجيب بيكر، بحط فيه ماء، وبحطه على اللهب أو الـ hot
+
+64
+00:04:21,610 --> 00:04:25,230
+plate. لما تغلي المياه، بروح بجيب الـ slide اللي
+
+65
+00:04:25,230 --> 00:04:28,550
+عملتلها خطوة smear preparation and air dry heat
+
+66
+00:04:28,550 --> 00:04:32,270
+fixation، وبحطها على طرف الـ beaker زي ما أنتم
+
+67
+00:04:32,270 --> 00:04:36,370
+شايفين بالصورة. بغمر الـ slide بالـ primary stain،
+
+68
+00:04:36,370 --> 00:04:40,950
+الـ Carbol fuchsin، وبخليها ٨ دقائق. cover the smear with
+
+69
+00:04:40,950 --> 00:04:45,450
+Carbol fuchsin steam over boiling water for 8 minutes.
+
+70
+00:04:45,450 --> 00:04:51,930
+بقول خلال ٨ دقائق، ممكن ألاحظ أن أطراف الـ slide جافة،
+
+71
+00:04:51,930 --> 00:04:56,430
+أنه بدأت تتبخر الصبغة، فبروُح بضيف Carbol fuchsin
+
+72
+00:04:56,430 --> 00:05:00,950
+(added additional stain).  في حالة stain was off، هاد مهم
+
+73
+00:05:00,950 --> 00:05:04,810
+جداً أني أضيف صبغة زيادة، لأنه لو جفت الـ slide،
+
+74
+00:05:04,810 --> 00:05:10,010
+هيتكون insoluble crystals. هذه الـ crystals راح يبين
+
+75
+00:05:10,010 --> 00:05:14,570
+شكلها بصلية ولونها أحمر، نفس لون الصبغة، بالتالي
+
+76
+00:05:14,570 --> 00:05:18,630
+هتعطيني false positive result. لأن الـ *Mycobacterium*
+
+77
+00:05:18,630 --> 00:05:22,310
+في الـ Acid-fast stain، شكلها بصلية، هتظل محتفظة
+
+78
+00:05:22,310 --> 00:05:25,430
+بالـ Carbol fuchsin، يعني لونها أحمر. هفكر عندي Acid
+
+79
+00:05:25,430 --> 00:05:30,210
+-fast bacilli، وهو ما في عندي فقط نتيجة غلط في الشغل.
+
+80
+00:05:30,210 --> 00:05:35,410
+الخطوة الثانية، بعد مرور ٨ دقائق، بقول لي بأطفئها،
+
+81
+00:05:35,410 --> 00:05:40,710
+وبأستنى إنها تبرد (after slide has cooled). ومهم جداً
+
+82
+00:05:40,710 --> 00:05:46,190
+الـ cooling (التبريد). التبريد هيعمل renaturation، إعادة
+
+83
+00:05:46,190 --> 00:05:50,570
+تكوين للروابط الكربونية اللي اتكسرت، بالتالي هتنحجز
+
+84
+00:05:50,570 --> 00:05:54,810
+الصبغة داخل البكتيريا. يعني الـ cooling، أنا ممكن
+
+85
+00:05:54,810 --> 00:05:59,710
+أعتبره as mordant، بالإضافة لشيء ثاني هنتعرف عليه.
+
+86
+00:05:59,710 --> 00:06:03,650
+هعتبره كمان كموردنت. يبقى أول شيء بأعتبره كموردنت
+
+87
+00:06:03,650 --> 00:06:07,030
+هو الـ cooling. الـ primary stain قلنا هي
+
+88
+00:06:07,030 --> 00:06:10,590
+Carbol fuchsin، لكن في الـ Gram stain كانت Crystal
+
+89
+00:06:10,590 --> 00:06:16,150
+violet. في الـ mordant في الـ Gram stain كان iodine،
+
+90
+00:06:16,150 --> 00:06:20,810
+لكن في الـ Acid-fast stain، الـ cooling هو as mordant.
+
+91
+00:06:20,810 --> 00:06:26,050
+بعدها بقول لي decolorized with acid-alcohol. بضيف الـ
+
+92
+00:06:26,050 --> 00:06:30,830
+decolorizer. بقول لي هون ما بعمل wash بالـ water زي الـ
+
+93
+00:06:30,830 --> 00:06:35,170
+Gram stain. على طول بضيف الـ decolorizer بعد ما تبرد
+
+94
+00:06:35,170 --> 00:06:41,310
+الـ slide. بحطها ١٥ second في acid-alcohol. في الـ
+
+95
+00:06:41,310 --> 00:06:44,990
+Gram stain كانت الـ decolorizer الكحول فقط، اللي هو
+
+96
+00:06:44,990 --> 00:06:48,710
+Ethanol، لكن في الـ Acid-fast stain، حمض الكحول،
+
+97
+00:06:48,710 --> 00:06:56,250
+الحمض ٣٪ HCl، والكحول ٩٥٪ Ethanol. بعد ما يمر الـ
+
+98
+00:06:56,250 --> 00:07:01,550
+١٥ second، بغسل بماء الصنبور، وأنتقل لخطوة بضيف الـ counter
+
+99
+00:07:01,550 --> 00:07:04,330
+stain أو الـ secondary stain، اللي هي في الـ Acid
+
+100
+00:07:04,330 --> 00:07:10,080
+-fast stain، الـ Methylene blue لمدة ٣٠ second. في الـ
+
+101
+00:07:10,080 --> 00:07:14,500
+Gram stain كانت عبارة عن Safranin. بغسل بعد مرور الـ
+
+102
+00:07:14,500 --> 00:07:19,220
+٣٠ second أو الـ one minute بالـ tap water، air
+
+103
+00:07:19,220 --> 00:07:23,500
+-drying. بشوفها تحت الميكروسكوب على عدسة ٤٠X وعدسة
+
+104
+00:07:23,500 --> 00:07:30,220
+١٠٠X اللي هي الـ oil immersion. الـ
+
+105
+00:07:30,220 --> 00:07:34,340
+reagents used are: Ziehl-Neelsen Carbol fuchsin، الـ
+
+106
+00:07:34,340 --> 00:07:37,760
+primary stain Carbol fuchsin، الـ Acid-alcohol
+
+107
+00:07:37,760 --> 00:07:42,480
+decolorizer، and Methylene blue، الـ secondary stain
+
+108
+00:07:42,480 --> 00:07:45,720
+أو الـ counter stain. قولنا الـ cooling is a
+
+109
+00:07:45,720 --> 00:07:49,860
+mordant. Acid-fast bacilli will be bright red after
+
+110
+00:07:49,860 --> 00:07:53,280
+staining. الـ Acid-fast bacilli اللي هي الـ
+
+111
+00:07:53,280 --> 00:07:57,660
+*Mycobacterium*، قولنا بتاخد اللون الأحمر، بتظهر
+
+112
+00:07:57,660 --> 00:08:02,000
+محتفظة بالـ primary stain، الـ Carbol fuchsin، لأن قولنا
+
+113
+00:08:02,000 --> 00:08:07,900
+هي صعبة صباغتها، لكن قدرنا نصبغها، وصعب إزالة اللون
+
+114
+00:08:07,900 --> 00:08:11,960
+منها لأنها مقاومة للـ decolorizer، الـ
+
+115
+00:08:11,960 --> 00:08:17,000
+decolorizer. قولنا حمض الكحول، الحمض ٣٪ HCl، مقاومة
+
+116
+00:08:17,000 --> 00:08:21,600
+للحمض القوي، ما راح يقدر يزيل اللون منها، فهتظل
+
+117
+00:08:21,600 --> 00:08:24,980
+محتفظة باللون الأحمر، بالـ primary stain.
+
+118
+00:08:34,060 --> 00:08:37,240
+Acid-fast stains are useful in the identification of
+
+119
+00:08:37,240 --> 00:08:40,860
+acid-fast bacilli. قلنا إن الـ Acid-fast stain هي
+
+120
+00:08:40,860 --> 00:08:44,660
+أداة مفيدة في تشخيص الـ Acid-fast bacilli اللي هي
+
+121
+00:08:44,660 --> 00:08:48,360
+الـ *Mycobacterium tuberculosis*. لكن الـ Gram stain
+
+122
+00:08:48,360 --> 00:08:51,180
+ما أقدر أقول إنها أداة مفيدة في الـ identification،
+
+123
+00:08:51,180 --> 00:08:55,660
+ما أقدر من خلالها أتعرف على هوية البكتيريا. الـ Gram
+
+124
+00:08:55,660 --> 00:08:59,440
+stain هي أول خطوة من خطوات تعريف البكتيريا، هي الـ
+
+125
+00:08:59,440 --> 00:09:03,710
+first step in identification. لكن عشان أعمل تعريف
+
+126
+00:09:03,710 --> 00:09:07,690
+للبكتيريا، لازم أزرع العينة على ميديا، وتنمو، وبعد
+
+127
+00:09:07,690 --> 00:09:11,090
+٢٤ ساعة أشوف نمو المستعمرات على الطبق، وأعمل
+
+128
+00:09:11,090 --> 00:09:14,790
+فحوصات كيميائية، من خلالها أتعرف على البكتيريا
+
+129
+00:09:14,790 --> 00:09:18,850
+الـ *Mycobacterium tuberculosis*. حتى تنمو بتحتاج من
+
+130
+00:09:18,850 --> 00:09:24,410
+٤ لـ ٦ أسابيع تقريباً. كل البكتيريا بتحتاج ٢٤ ساعة
+
+131
+00:09:24,410 --> 00:09:28,070
+فقط حتى تنمو، لكن الـ *Mycobacterium tuberculosis*
+
+132
+00:09:28,070 --> 00:09:33,280
+بتحتاج من ٤ لـ ٦ أسابيع حتى تنمو. نتيجة وجود الـ waxy
+
+133
+00:09:33,280 --> 00:09:37,480
+lipids، الـ waxy lipids بيعمل كحاجز بيمنع وصول
+
+134
+00:09:37,480 --> 00:09:42,720
+المغذيات للبكتيريا، فبتاخد وقت أطول حتى تنمو. عشان
+
+135
+00:09:42,720 --> 00:09:48,360
+هيك الدكتور بيطلب فحص الـ Acid-fast stain كفحص سريع،
+
+136
+00:09:48,360 --> 00:09:52,820
+سريع. مش هيستنى ٦ أسابيع يستنى نمو البكتيريا، خلال
+
+137
+00:09:52,820 --> 00:09:57,740
+نصف ساعة بتطلع نتيجة الـ Acid-fast stain. preliminary
+
+138
+00:09:57,740 --> 00:10:03,780
+diagnosis of tuberculosis. هو فحص أولي، لأنه لازم أنا
+
+139
+00:10:03,780 --> 00:10:07,840
+بعدين أعمل مزرعة، أزرع البكتيريا وتنمو، وأعمل
+
+140
+00:10:07,840 --> 00:10:11,260
+فحوصات كيميائية عشان أتأكد إنه هي *Mycobacterium*
+
+141
+00:10:11,260 --> 00:10:15,740
+tuberculosis.  and the greater than 90% predictive
+
+142
+00:10:15,740 --> 00:10:19,880
+value from sputum sample. بقول لي إنه لو أنت عملتي
+
+143
+00:10:19,880 --> 00:10:24,780
+الـ Acid-fast stain وطلع Acid-fast bacilli  ٩٠٪، بنحكي
+
+144
+00:10:24,780 --> 00:10:28,500
+أنه هي البكتيريا *Mycobacterium tuberculosis*. طب ليش
+
+145
+00:10:28,500 --> 00:10:32,870
+مش ١٠٠٪؟ لأنه لازم نزرع البكتيريا ونشوف نمو
+
+146
+00:10:32,870 --> 00:10:36,690
+البكتيريا على الطبق، ونعمل فحوصات نتأكد إنها
+
+147
+00:10:36,690 --> 00:10:41,770
+*Mycobacterium tuberculosis*. هيك بأحكي إنها نسبة ١٠٠٪.
+
+148
+00:10:41,770 --> 00:10:46,050
+وحتى نتيجة الـ Acid-fast stain ما بنكتب النتيجة الـ
+
+149
+00:10:46,050 --> 00:10:49,030
+positive لو كانت بصلية لونها أحمر. ما بكتب
+
+150
+00:10:49,030 --> 00:10:54,130
+*Mycobacterium* غلط، بنكتب Acid-fast bacilli. بصلية
+
+151
+00:10:54,130 --> 00:10:58,640
+لأن *Mycobacterium tuberculosis* شكلها بصلية. الـ
+
+152
+00:10:58,640 --> 00:11:03,140
+Acid-fast معناها إنها بتقاوم الحمض، الحمض اللي هو ٣
+
+153
+00:11:03,140 --> 00:11:10,360
+٪ HCl، الـ decolorizer. عشان هي احتفظت باللون، لون
+
+154
+00:11:10,360 --> 00:11:13,820
+الـ Carbol fuchsin،  primary stain، وما قدر حتى الحمض
+
+155
+00:11:13,820 --> 00:11:18,420
+القوي، الـ HCl، يزيل هذا اللون. طبعاً هذه الخاصية إنها
+
+156
+00:11:18,420 --> 00:11:22,020
+بتقاوم الحمض، خاصية من خصائص الـ *Mycobacterium*
+
+157
+00:11:22,020 --> 00:11:25,660
+tuberculosis. من خلال هذه الخاصية قدرت أتعرف على
+
+158
+00:11:25,660 --> 00:11:30,930
+البكتيريا. كيف أنا بدي أكتب نتيجة الـ Acid-fast stain؟
+
+159
+00:11:30,930 --> 00:11:34,610
+من خلال هذا الجدول هنتعرف. بقول لك لو فحصتي في الـ
+
+160
+00:11:34,610 --> 00:11:39,690
+field وما شفتي أي Acid-fast bacilli، بصلية، شكلها بصلية،
+
+161
+00:11:39,690 --> 00:11:44,410
+Acid-fast، يعني بتاخد اللون الأحمر، بتكتبي نتيجة: no
+
+162
+00:11:44,410 --> 00:11:48,710
+acid-fast bacilli. ممنوع تكتبي negative، لأنه ممكن
+
+163
+00:11:48,710 --> 00:11:54,290
+يكون شغلك غلط، ممكن طريقة جمعك للعينة غلط. تكتبي no
+
+164
+00:11:54,290 --> 00:11:58,870
+acid-fast bacilli، معناها هذه الجملة إنك أنت فحصتي بالـ
+
+165
+00:11:58,870 --> 00:12:03,910
+field وما شفتي أي بكتيريا. لو فحصتِ ٣٠٠ field وكانت
+
+166
+00:12:03,910 --> 00:12:09,150
+البكتيريا من ١ لـ ٢ acid-fast bacilli، بتكون
+
+167
+00:12:09,150 --> 00:12:13,270
+العينة doubtful، مشكوك فيها، بقول لك عيدي الفحص الـ
+
+168
+00:12:13,270 --> 00:12:17,430
+Acid-fast stain مرة ثانية. لو فحصتِ ١٠٠ field وكان
+
+169
+00:12:17,430 --> 00:12:22,740
+من ١ لـ ٩ acid-fast bacilli، بتعطي +١. لو عشرين
+
+170
+00:12:22,740 --> 00:12:25,840
+في الـ field حصلت، وكان Acid-fast bacilli من واحد
+
+171
+00:12:25,840 --> 00:12:29,620
+لتسعة، +٢. لو field واحد كان فيه من واحد لتسعة
+
+172
+00:12:29,6
+
+223
+00:16:11,940 --> 00:16:15,640
+يزيل اللون منها لأنّ صبغة الكاربولفوكسين
+
+224
+00:16:15,640 --> 00:16:20,740
+الكاربولفوكسين ثابتة أصلاً في  الواكس، ولذلك لن أقدر
+
+225
+00:16:20,740 --> 00:16:24,040
+أن أفصل الـCarbolfoxin عن الـWax Lipid  مثل السكر
+
+226
+00:16:24,040 --> 00:16:27,160
+لما يدوب في الماء لا أقدر أفصلهم عن بعض، فلما يجي الـ
+
+227
+00:16:27,160 --> 00:16:31,740
+Decolorizer بعدها سيكون الـCarbolfuchsin ثابت في الـ
+
+228
+00:16:31,740 --> 00:16:36,100
+Wax Lipid،  لن يقدر يزيل اللون، لكنّ الـNon-acid
+
+229
+00:16:36,100 --> 00:16:40,540
+fast organism لا تحتوي على Wax Lipid، فأول شيء
+
+230
+00:16:40,540 --> 00:16:44,180
+أصبغها بـPrimer،  ثمّ Carbolfoxin ستأخذ الصبغة بكلّ
+
+231
+00:16:44,180 --> 00:16:47,960
+سهولة، بعدها نضيف الـDecolorizer  (Acid Alcohol)
+
+232
+00:16:48,430 --> 00:16:52,190
+الـCarbolfoxin سيدوب في الـacid alcohol لأنّني
+
+233
+00:16:52,190 --> 00:16:55,890
+لا أملك wax lipid، فاللون سيروح بكل سهولة وستصبح
+
+234
+00:16:55,890 --> 00:16:59,850
+شفّافة،  سأضطرّ أصبغها بـcounter stain with methylene
+
+235
+00:16:59,850 --> 00:17:02,650
+blue،  الـmethylene blue هو الـcounter stain which
+
+236
+00:17:02,650 --> 00:17:05,850
+لا تستطيع اختراق mycolic acid (لا تستطيع اختراق الـ)
+
+237
+00:17:05,850 --> 00:17:08,730
+mycolic acid (الـwaxy lipid) لأنّها ليست lipid
+
+238
+00:17:08,730 --> 00:17:13,030
+soluble مثل الـCarbolfoxin، ولم أعرض الصبغة للبخار
+
+239
+00:17:13,030 --> 00:17:17,010
+ليوفر تبايناً للخلايا غير سريعة الحمض، أقول لك هذا
+
+240
+00:17:18,070 --> 00:17:23,790
+لتمييزها، وهي تعطي اللون للخلايا غير سريعة الحمض، و
+
+241
+00:17:23,790 --> 00:17:27,390
+أستطيع أن أفرّق بين سريعة الحمض وغير سريعة الحمض، لكنّ
+
+242
+00:17:27,390 --> 00:17:31,850
+سريعة الحمض، مثل الميكوبكتيريا، يصعب صباغتها،
+
+243
+00:17:31,850 --> 00:17:35,970
+but once stained، الميكوبكتيريا يصعب صباغتها، لكن  إذا صبغت
+
+244
+00:17:35,970 --> 00:17:43,000
+إذا استطعت صباغتها، كما فعلنا في المختبر، يصعب إزالة
+
+245
+00:17:43,000 --> 00:17:47,820
+اللون منها حتى في وجود
+
+246
+00:17:47,820 --> 00:17:53,460
+حمض قويّ، يصعب أن نزيل اللون من الميكوبكتيريا
+
+247
+00:17:53,460 --> 00:17:58,120
+وهي طبعاً خاصية أو ميزة من خلالها أستطيع التعرّف على
+
+248
+00:17:58,120 --> 00:18:03,000
+البكتيريا بالنسبة
+
+249
+00:18:03,000 --> 00:18:06,180
+للخطوات: تحضير مسحة، تجفيف بالهواء، تثبيت بالحرارة
+
+250
+00:18:06,180 --> 00:18:11,240
+(برايمر، كاربوفوكسين حتى تساعد)
+
+251
+00:18:11,240 --> 00:18:15,680
+اختراق الكاربوفوكسين، أضيف تيرجيتول لمدة
+
+252
+00:18:15,680 --> 00:18:20,560
+خمس دقائق،  وإذا لم يكن لديّ تيرجيتول، أعرض الشريحة للـ
+
+253
+00:18:20,560 --> 00:18:26,120
+بخار لمدة ثمان دقائق، بعدها أقوم بالتّبريد
+
+254
+00:18:26,120 --> 00:18:31,380
+لشريحة،  أستخدم كحول حامض وهو الـ
+
+255
+00:18:31,380 --> 00:18:35,340
+Decolorizer لمدة خمس عشرة إلى عشرين ثانية، بعدها أغسل
+
+256
+00:18:35,340 --> 00:18:39,650
+ب الماء، أضيف الـcounter stain المناسب لمدة دقيقة واحدة
+
+257
+00:18:39,650 --> 00:18:43,250
+أو ثلاثين ثانية، أغسل بالماء من الصنبور، تجفيف بالهواء،
+
+258
+00:18:43,250 --> 00:18:46,050
+وأشاهدها تحت المجهر باستخدام زيت الغمر
+
+259
+00:18:46,050 --> 00:18:49,910
+النتيجة الإيجابية: زوج من الخلايا باللون الوردي أو الأحمر
+
+260
+00:18:49,910 --> 00:18:54,190
+يجب أن تكون البكتيريا السالبة تظهر باللون
+
+261
+00:18:54,190 --> 00:18:56,590
+الأزرق، كحزم أو على شكل خطوط
+
+262
+00:19:00,870 --> 00:19:05,210
+قبل الصباغة، تكون البكتيريا شفافة، صبغت
+
+263
+00:19:05,210 --> 00:19:09,310
+بالكاربولفوكسين (صبغة أوليّة)، سواء كانت سريعة الحمض
+
+264
+00:19:09,310 --> 00:19:13,490
+أو غير سريعة الحمض، ستأخذ اللون
+
+265
+00:19:13,490 --> 00:19:16,790
+الأحمر، بعدها أضفت المُزيل (اللون) وهو الحمض
+
+266
+00:19:16,790 --> 00:19:21,430
+الكحولي، سينزع اللون من غير سريعة الحمض لأنّ
+
+267
+00:19:21,430 --> 00:19:25,940
+سرعة ذوبان الكاربولفوكسين في الـwax lipid أعلى
+
+268
+00:19:25,940 --> 00:19:29,540
+من الحمض الكحولي بالنسبة لـNon-acid fast bacilli
+
+269
+00:19:29,540 --> 00:19:33,300
+لا تحتوي على wax lipid، بالتالي الكاربولفوكسين سيدوب
+
+270
+00:19:33,300 --> 00:19:37,600
+في الحمض الكحولي، واللون سيذهب، لكنّ سريعة الحمض
+
+271
+00:19:37,600 --> 00:19:42,080
+البكتيريا تحتوي على wax lipid، الكاربولفوكسين
+
+272
+00:19:42,080 --> 00:19:45,660
+سيدوب في الـwax lipid، سيأتي المُزيل، لن
+
+273
+00:19:45,660 --> 00:19:49,260
+يستطيع إزالة اللون، بعدها أضيف الصبغة المُقابلّة
+
+274
+00:19:49,260 --> 00:19:54,040
+الميثيلين بلو،  أكيد البكتيريا التي كانت شفافة
+
+275
+00:19:54,040 --> 00:19:57,640
+شفافة، ستأخذ اللون، وهو اللون الأزرق، لكن
+
+276
+00:19:57,640 --> 00:20:01,280
+البكتيريا سريعة الحمض ستبقى محافظة
+
+277
+00:20:01,280 --> 00:20:05,780
+على اللون الأحمر، لأنّا قلنا أنّها يصعب إزالة
+
+278
+00:20:05,780 --> 00:20:10,500
+اللون منها، بالتالي ستبقى حمراء،  لكنّ
+
+279
+00:20:10,500 --> 00:20:16,200
+غير سريعة الحمض قد تكون كرويّة أو عصويّة
+
+280
+00:20:16,200 --> 00:20:18,460
+لكنّ اللون أزرق
+
+281
+00:20:22,120 --> 00:20:26,440
+هذه نتائج صبغة سريعة الحمض، نلاحظ في كلّ الصور أنّها سريعة
+
+282
+00:20:26,440 --> 00:20:31,220
+الحمض،  هكذا أكتب النتيجة: سريعة الحمض، عصوية،
+
+283
+00:20:31,220 --> 00:20:36,040
+الشكل تبعها عصوي، وسريعة الحمض، يعني مقاومة
+
+284
+00:20:36,040 --> 00:20:41,900
+للحمض (3% HCL decolorizer)، احتفظت بلون الصبغة الأوليّة
+
+285
+00:20:41,900 --> 00:20:46,720
+(الكربوفوكسين)، ومازال حتى الحمض القوي لا يزيل اللون
+
+286
+00:20:46,720 --> 00:20:47,180
+منها
+
+287
+00:20:51,330 --> 00:20:54,970
+صبغة كينون أو الطريقة الباردة لصبغ
+
+288
+00:20:54,970 --> 00:20:58,970
+الكائنات الدقيقة سريعة الحمض، هي طريقة لصبغ سريعة
+
+289
+00:20:58,970 --> 00:21:03,350
+الحمض، خاصة الميكوبكتيريا، الإجراء مشابه
+
+290
+00:21:03,350 --> 00:21:07,470
+لصبغة زيل نيلسون، الخطوات هي نفس خطوات زيل
+
+291
+00:21:07,470 --> 00:21:10,850
+نيلسون، وهي صبغة سريعة الحمض، لكن
+
+292
+00:21:10,850 --> 00:21:14,610
+لا تتضمن تسخين الشريحة، لكنّني لا أعرض
+
+293
+00:21:14,610 --> 00:21:17,770
+الشريحة للبخار، لهذا سُمّيت بالطريقة الباردة
+
+294
+00:21:18,400 --> 00:21:22,440
+طريقة صباغة كينون، تستخدم الكاربوكسين كصبغة أوليّة
+
+295
+00:21:22,440 --> 00:21:26,000
+أستخدم الكاربولفوكسين كصبغة أوليّة، حمض
+
+296
+00:21:26,000 --> 00:21:29,820
+كحولي كمزيل، والميثيلين بلو كصبغة مقابلّة
+
+297
+00:21:29,820 --> 00:21:34,080
+طيب، ما الفرق؟  كينون كاربولفوكسين يحتوي على
+
+298
+00:21:34,080 --> 00:21:37,340
+تركيز أعلى من الفينول ومن الـbasicfuchsin
+
+299
+00:21:37,340 --> 00:21:41,760
+يقولون أنّ فيه تركيز عالٍ من الفينول ومن الـ
+
+300
+00:21:41,760 --> 00:21:45,760
+basicfuchsin، وهما أحد مكونات الكاربولفوكسين
+
+301
+00:21:45,760 --> 00:21:50,980
+بالتالي، لا أحتاج الحرارة حتى تخترق
+
+302
+00:21:50,980 --> 00:21:55,760
+الطبقة الشمعيّة، تحت المجهر أشاهد الخلايا الـ
+
+303
+00:21:55,760 --> 00:21:58,680
+سريعة الحمض تظهر باللون الأحمر، وغير سريعة
+
+304
+00:21:58,680 --> 00:22:03,340
+الحمض تظهر باللون الأزرق، نفس نتائج
+
+305
+00:22:03,340 --> 00:22:09,940
+صبغة سريعة الحمض، أورامين روديمين
+
+306
+00:22:10,310 --> 00:22:13,670
+صبغة أورامين روديمين الفلورية، هي الطريقة
+
+307
+00:22:13,670 --> 00:22:17,130
+الثالثة لأرى البكتيريا سريعة الحمض
+
+308
+00:22:17,130 --> 00:22:20,650
+وهي الميكوبكتيريا، الطريقة الأولى كانت
+
+309
+00:22:20,650 --> 00:22:23,470
+صبغة سريعة الحمض أو صبغة زيل نيلسون، الطريقة
+
+310
+00:22:23,470 --> 00:22:26,290
+الثانية كانت صبغة كينون، والطريقة الثالثة
+
+311
+00:22:26,290 --> 00:22:30,030
+وهي صبغة أورامين روديمين، من خلال هذه
+
+312
+00:22:30,030 --> 00:22:34,090
+الطريقة أستطيع رؤية البكتيريا سريعة الحمض باستخدام
+
+313
+00:22:34,090 --> 00:22:37,650
+المجهر الفلوري، ستظهر الميكوبكتيريا بلون
+
+314
+00:22:37,650 --> 00:22:39,150
+أصفر مائل إلى الأحمر
+
+315
+00:22:42,740 --> 00:22:46,980
+آخر معلومة في صبغة سريعة الحمض، اللون الوردي المحمر
+
+316
+00:22:46,980 --> 00:22:50,720
+والخلايا ملتصقة مع بعضها البعض على شكل خيوط طويلة
+
+317
+00:22:50,720 --> 00:22:54,320
+كتل من خلايا الميكوبكتيريا، بسبب الزيادة
+
+318
+00:22:54,320 --> 00:22:57,240
+في الدهون في جدار الخلية،  يقولون أنّ هناك طبقة
+
+319
+00:22:57,240 --> 00:23:01,280
+شمعيّة، تجعل البكتيريا تلتصق ببعضها، وتظهر على شكل
+
+320
+00:23:01,280 --> 00:23:05,780
+خيوط، تمام؟ هذه الظاهرة تُسمّى كتل  
+
+321
+00:23:05,780 --> 00:23:10,730
+أو يُمكن تسميتها خيوط، هذه الظاهرة تظهر في
+
+322
+00:23:10,730 --> 00:23:14,790
+الميكوبكتيريا نتيجة التركيز العالي لـ
+
+323
+00:23:14,790 --> 00:23:15,410
+الدهون
diff --git a/PL9fwy3NUQKwZWgAb_V1ATlRRsH-6i3cPC/4-SUdsxRvPI_raw.srt b/PL9fwy3NUQKwZWgAb_V1ATlRRsH-6i3cPC/4-SUdsxRvPI_raw.srt
new file mode 100644
index 0000000000000000000000000000000000000000..2889675ff7eba68f5619b5d823b636b964bee3c7
--- /dev/null
+++ b/PL9fwy3NUQKwZWgAb_V1ATlRRsH-6i3cPC/4-SUdsxRvPI_raw.srt
@@ -0,0 +1,1292 @@
+1
+00:00:01,480 --> 00:00:05,740
+Acid Fast Stain أو الإسم الآخر Zell-Nelson Stain
+
+2
+00:00:05,740 --> 00:00:10,360
+علمان اكتشفوا هذه الصبغة فصميت باسمها The Acid
+
+3
+00:00:10,360 --> 00:00:13,180
+Fast Stain is another differential staining method
+
+4
+00:00:13,180 --> 00:00:15,900
+اتفقنا إنه هي نوع من أنواع الـDifferential Stain
+
+5
+00:00:15,900 --> 00:00:19,900
+اللي هتقسم البكتيريا لمجموعة فهتقسم البكتيريا ل
+
+6
+00:00:19,900 --> 00:00:24,640
+Acid Fast بس اللهي وNon Acid Fast بس اللهي في
+
+7
+00:00:24,640 --> 00:00:27,540
+الإجرام الستان قلنا إنه مش كل البكتيريا بقدر
+
+8
+00:00:27,540 --> 00:00:30,770
+أصبغهافي عندي استثناء في عندي بكتيريا الـ
+
+9
+00:00:30,770 --> 00:00:34,750
+Mycobacterium هذه البكتيريا ما بقدر أصبغها بالـ
+
+10
+00:00:34,750 --> 00:00:38,610
+Gram Stain عشان هيك بستخدم ال Acid Fast Stain
+
+11
+00:00:38,610 --> 00:00:43,210
+لصيباغتها in this case the target cell are usually
+
+12
+00:00:43,210 --> 00:00:46,310
+members of the genus Mycobacterium ال
+
+13
+00:00:46,310 --> 00:00:49,430
+Mycobacterium هي اللي بستهدفها في ال Acid Fast
+
+14
+00:00:49,430 --> 00:00:53,430
+Stain طيب ليش أنا ماقدر أصبغ ال Mycobacterium بال
+
+15
+00:00:53,430 --> 00:00:57,380
+Gram Stain أو حتى بال symbol Stainبقول لي لأن هاي
+
+16
+00:00:57,380 --> 00:01:00,760
+البكتيريا ال cell wall تبعها في high concentration
+
+17
+00:01:00,760 --> 00:01:04,680
+of wax celibate ال wax celibate هاي بتكون كحاجز
+
+18
+00:01:04,680 --> 00:01:09,280
+بتمنع دخول الصبغة داخل البكتيريا وحتى لو أنا قدرت
+
+19
+00:01:09,280 --> 00:01:13,440
+أصبغها صعب أني أنا أزيل اللعب the cell walls of
+
+20
+00:01:13,440 --> 00:01:16,740
+these bacteria contain an unusually high
+
+21
+00:01:16,740 --> 00:01:19,660
+concentration of wax celibate the smoking
+
+22
+00:01:19,660 --> 00:01:22,440
+conventional symbol stand and the ground stand
+
+23
+00:01:22,440 --> 00:01:27,190
+uselessهذا بيجعل صعب صباغتها بالـ Gram Stain
+
+24
+00:01:27,190 --> 00:01:31,190
+والسمبل Stain The genus Mycobacterium الـ
+
+25
+00:01:31,190 --> 00:01:35,170
+Mycobacterium هو يعتبر genus content two important
+
+26
+00:01:35,170 --> 00:01:39,830
+human pathogen في عندي في هذه العائلة two species
+
+27
+00:01:39,830 --> 00:01:44,010
+مهمين بيسببوا مرض للإنسان الـ Mycobacterium
+
+28
+00:01:44,010 --> 00:01:47,870
+tuberculosis اللي بيسبب الصلق الرؤوي والـ
+
+29
+00:01:47,870 --> 00:01:52,430
+Mycobacterium liberi هاي اللي بتسبب الجذعهو مرض
+
+30
+00:01:52,430 --> 00:01:57,170
+بيكون فيه ندب بالجلد skin scar وبيصير فيه تدمير
+
+31
+00:01:57,170 --> 00:02:02,750
+بالجهاز العصبي brain damage ال M رمز لجيناس
+
+32
+00:02:02,750 --> 00:02:05,770
+Mycobacterium ال tuberculosis هي ال species
+
+33
+00:02:05,770 --> 00:02:10,570
+الكتابة العلمية للبكتيريا لازم ان انا احط خط او
+
+34
+00:02:10,570 --> 00:02:18,070
+انها تكون الكتابة مائلة هبدأ
+
+35
+00:02:18,070 --> 00:02:22,600
+بشرح ال procedure لان من خلالها هنتعرفكيف بدي أقدر
+
+36
+00:02:22,600 --> 00:02:27,420
+أصبغ هاي البكتيريا اللي صعب صباغتها أي استان في
+
+37
+00:02:27,420 --> 00:02:32,000
+معمل الـ Microbiology قولنا خطوات الصباغة، Smear
+
+38
+00:02:32,000 --> 00:02:35,960
+preparation، Air-drying، Heat-fixation، وستان،
+
+39
+00:02:35,960 --> 00:02:40,780
+استان بتختلف حسب نوع الصباغة هنتكلم على الـ Acid
+
+40
+00:02:40,780 --> 00:02:44,140
+-fast stain في الـ Gram stain الـ Primer stain
+
+41
+00:02:44,140 --> 00:02:47,600
+كانت لـ Crystal Violet لكن في الـ Acid-fast stain
+
+42
+00:02:47,600 --> 00:02:52,220
+هيختلف اسمها وهتختلف طريقة صباغتهاالـ Primer Stain
+
+43
+00:02:52,220 --> 00:02:56,180
+في الـ Acid-Fast Stain Carbolfoxin الـ Carbolfoxin
+
+44
+00:02:56,180 --> 00:02:59,820
+إذا متذكرين في معمل الـ Simplestain قلنا هي Basic
+
+45
+00:02:59,820 --> 00:03:04,460
+Stain لكن اللي بيميز الـ Carbolfoxin إنها Lipid
+
+46
+00:03:04,460 --> 00:03:07,800
+-Soluble والتي احنا قلنا إنه الـ Mycobacterium
+
+47
+00:03:07,800 --> 00:03:11,340
+بتحتوي على كمية كبيرة من الدهون عشان هيك ما بقدر
+
+48
+00:03:11,340 --> 00:03:15,520
+أصبغها بالSimplestain أو بالجرام Stain من خلال هذه
+
+49
+00:03:15,520 --> 00:03:21,440
+الخاصية هتساعد على اختراق الصبغة للـ Waxy Lipidلكن
+
+50
+00:03:21,440 --> 00:03:24,880
+بقولنا إن الاشي الرئيس اللي هيساعد على اختراق الـ
+
+51
+00:03:24,880 --> 00:03:29,980
+Carbolfoxin لـ Wax Libid هو الـ Steam شو يعني
+
+52
+00:03:29,980 --> 00:03:34,620
+Steam؟ الـ Steam هو البخار، عرض الصبغة لبخار ليش
+
+53
+00:03:34,620 --> 00:03:38,120
+عرض الصبغة لبخار؟ بقولّي البخار هيعمل الـ
+
+54
+00:03:38,120 --> 00:03:42,240
+Denaturation للروابط الكربونية الموجودة في الـ
+
+55
+00:03:42,240 --> 00:03:46,580
+Mycolic Acid اللي هو الـ Wax Libid بالتالي هيساعد
+
+56
+00:03:46,580 --> 00:03:52,100
+على اختراق الصبغة للـ Wax Libidهك انا اقدرت اصبغ
+
+57
+00:03:52,100 --> 00:03:55,340
+هذه البكتيريا من خلال استخدام الـ Primers تاني
+
+58
+00:03:55,340 --> 00:03:59,360
+الكربوفوكسين اللي هي Lipid Soluble الاشي الأساسي
+
+59
+00:03:59,360 --> 00:04:05,000
+من خلاله اقدرت اصبغ اللي هو تعريض البكتيريا لـ
+
+60
+00:04:05,000 --> 00:04:08,720
+Steam لبخار هيكسر الروابط الكربونية الموجودة في
+
+61
+00:04:08,720 --> 00:04:12,960
+الـ Mycolic Acid بالتالي هيساعد على اختراق الصبغة
+
+62
+00:04:12,960 --> 00:04:17,500
+للـ Wax Celibate كيف انا بدي اعمل هذه الخطوة بروح
+
+63
+00:04:17,500 --> 00:04:21,610
+بجيب بيكر بحط فيه ميهو بحطه على اللهب او الـ hot
+
+64
+00:04:21,610 --> 00:04:25,230
+plate لما تغلي الميه بروح بجيب ال slide اللي
+
+65
+00:04:25,230 --> 00:04:28,550
+عملتلها خطوة سمير preparation and dry heat
+
+66
+00:04:28,550 --> 00:04:32,270
+fixation و بحطها على طرف ال بيكر زي ما انتوا
+
+67
+00:04:32,270 --> 00:04:36,370
+شايفين بالصورة بغمر ال slide بالبراي مارستان
+
+68
+00:04:36,370 --> 00:04:40,950
+الكاربولفوكسين و بحسب 8 دقائق cover السمير with
+
+69
+00:04:40,950 --> 00:04:45,450
+carbolfoxin steam over boiling water for 8 minutes
+
+70
+00:04:45,450 --> 00:04:51,930
+بقول خلال 8 دقائقممكن ألاحظ أن أطراف ال slide جافة
+
+71
+00:04:51,930 --> 00:04:56,430
+أنه بدأت تتبخر الصبغة فبروح بضيف Carbol Fucsin
+
+72
+00:04:56,430 --> 00:05:00,950
+Added Additional Stain في Stain Was Off هان مهم
+
+73
+00:05:00,950 --> 00:05:04,810
+جدا أن أنا أضيف صبغة لأس لأنه لو جفت ال slide
+
+74
+00:05:04,810 --> 00:05:10,010
+هيتكون Insoluble Crystal هذه ال crystal راح يبين
+
+75
+00:05:10,010 --> 00:05:14,570
+شكلها بصلاي ولونها أحمر نفس لون الصبغة بالتالي
+
+76
+00:05:14,570 --> 00:05:18,630
+هتعطيني False Positive Resultلأن الـ Mycobacterium
+
+77
+00:05:18,630 --> 00:05:22,310
+في الـ Acid Fast Stain شكلها بصلاي هتظل محتفظة
+
+78
+00:05:22,310 --> 00:05:25,430
+بالـ Carbolfoxin يعني لونها أحمر هفكر عندي Acid
+
+79
+00:05:25,430 --> 00:05:30,210
+Fast بصلاي هو مافي عندي فقط نتيجة غلط في الشغل
+
+80
+00:05:30,210 --> 00:05:35,410
+الخطوة التانية بعد مرور 8 دقائق بقولي باطفل لها
+
+81
+00:05:35,410 --> 00:05:40,710
+وبستنقل عينها تبرد after slide has cooled ومهم جدا
+
+82
+00:05:40,710 --> 00:05:46,190
+ال cooling التبريدالتبريد هيعمل Renaturation إعادة
+
+83
+00:05:46,190 --> 00:05:50,570
+رابط الروابط الكربونية اللي اتكسرت بالتالي هتنحجز
+
+84
+00:05:50,570 --> 00:05:54,810
+الصبغة داخل البكتيريا يعني ال cooling أنا ممكن
+
+85
+00:05:54,810 --> 00:05:59,710
+أعتبره as Mordant بالإضافة لإشي تاني هنتعرف عليه
+
+86
+00:05:59,710 --> 00:06:03,650
+هيعتبر كمان ك Mordant يبقى أول إشي باعتبره ك
+
+87
+00:06:03,650 --> 00:06:07,030
+Mordant هو ال cooling ال primary stain قلنا هي
+
+88
+00:06:07,030 --> 00:06:10,590
+Carbolfoxin لكن في ال gram stain كانت crystal
+
+89
+00:06:10,590 --> 00:06:16,150
+violetفي الـ Mordant في الـ gram stain كان iodine
+
+90
+00:06:16,150 --> 00:06:20,810
+لكن في ال acid fast stain ال cooling هو as Mordant
+
+91
+00:06:20,810 --> 00:06:26,050
+بعدها بقولي decalorized with acid alcohol بضيف ال
+
+92
+00:06:26,050 --> 00:06:30,830
+decalorizer بقولي هان ما بعمل wash بال water زي ال
+
+93
+00:06:30,830 --> 00:06:35,170
+gram stain على طول بضيف ال decalorizer بعد ما تبرد
+
+94
+00:06:35,170 --> 00:06:41,310
+ال slime بحطها 15 second في acid alcoholفي الـ
+
+95
+00:06:41,310 --> 00:06:44,990
+Gram Stain كانت الـ Decalarizer ألكحول فقط اللي هو
+
+96
+00:06:44,990 --> 00:06:48,710
+Ethanol لكن في الـ Acid Fast Stain أسد ألكحول
+
+97
+00:06:48,710 --> 00:06:56,250
+الأسد 3% HCl والألكحول 95% Ethanol بعد ما مرور الـ
+
+98
+00:06:56,250 --> 00:07:01,550
+15 Second بغسل بطاب و أترق بخطوة بضيف الـ Counter
+
+99
+00:07:01,550 --> 00:07:04,330
+Stain أو الـ Secondary Stain اللي هي في الـ Acid
+
+100
+00:07:04,330 --> 00:07:10,080
+Fast Stain الـ Methylene Blue لمدة 30 Secondفي الـ
+
+101
+00:07:10,080 --> 00:07:14,500
+Gram Stain كانت عبارة عن صفرانين بغسل بعد مرور الـ
+
+102
+00:07:14,500 --> 00:07:19,220
+30 second أو الـ one minute بالـ tap water air
+
+103
+00:07:19,220 --> 00:07:23,500
+-drying بشوفها تحت الميكروسكوب على عدسة 40X وعدسة
+
+104
+00:07:23,500 --> 00:07:30,220
+100X اللي هي الـ oil immersion الـ
+
+105
+00:07:30,220 --> 00:07:34,340
+reagents used are Diel-Nelson Carbolfoxane الـ
+
+106
+00:07:34,340 --> 00:07:37,760
+Primary Stain Carbolfoxaneالـ Acid Alcohol
+
+107
+00:07:37,760 --> 00:07:42,480
+Decolorizer and Methylene Blue الـ Secondary Stain
+
+108
+00:07:42,480 --> 00:07:45,720
+أو الـ Counter Stain قولنا الـ Cooling is more
+
+109
+00:07:45,720 --> 00:07:49,860
+dense Acid Phospholipase will be bright red after
+
+110
+00:07:49,860 --> 00:07:53,280
+staining الـ Acid Phospholipase اللي هي الـ
+
+111
+00:07:53,280 --> 00:07:57,660
+Mycobacterium قولنا بتاخد اللون الأحمر بتظهر
+
+112
+00:07:57,660 --> 00:08:02,000
+محتفظة بالـ Primary Stain الكاربولفوكسين لأن قولنا
+
+113
+00:08:02,000 --> 00:08:07,900
+هي صعبة صبغتها لكن قدرنا نصبغهاوصعب إزالة اللون
+
+114
+00:08:07,900 --> 00:08:11,960
+منها لأنها مقاومة للحمد الحمد اللي هو الـ
+
+115
+00:08:11,960 --> 00:08:17,000
+Decalarizer قولنا أسد الكحول الأسد 3% HCl مقاومة
+
+116
+00:08:17,000 --> 00:08:21,600
+للحمد القوي ما راح يقدر يزيل اللون منها فهتظل
+
+117
+00:08:21,600 --> 00:08:24,980
+محتفظة باللون الأحمر بالـ Primary Stain
+
+118
+00:08:34,060 --> 00:08:37,240
+Acid fast stain are useful in identification of
+
+119
+00:08:37,240 --> 00:08:40,860
+acid fast bacilli قلنا إن الـ Acid fast stain هي
+
+120
+00:08:40,860 --> 00:08:44,660
+أداة مفيدة في تشخيص الـ Acid fast bacilli اللي هي
+
+121
+00:08:44,660 --> 00:08:48,360
+الـ Mycobacterium tuberculosis لكن لـ Gram stain
+
+122
+00:08:48,360 --> 00:08:51,180
+ما بقدر أقول إنها أداة مفيدة في ال identification
+
+123
+00:08:51,180 --> 00:08:55,660
+ما بقدر من خلالها أتعرف على هوية البكتيريا لـ Gram
+
+124
+00:08:55,660 --> 00:08:59,440
+stain هي أول خطوة من خطوات تعريف البكتيريا هي ال
+
+125
+00:08:59,440 --> 00:09:03,710
+first step in identificationلكن عشان أعمل تعريف
+
+126
+00:09:03,710 --> 00:09:07,690
+للبكتيريا لازم أسرع العين على ميديا و تنمو و بعد
+
+127
+00:09:07,690 --> 00:09:11,090
+24 ساعة أشوف نمو المستعمرات على الطبق و أشرف
+
+128
+00:09:11,090 --> 00:09:14,790
+الحصاد الكيميائية من خلالها أتعرف على البكتيريا
+
+129
+00:09:14,790 --> 00:09:18,850
+الـ Mycobacterium tuberculosis حتى تنمو بتحتاج من
+
+130
+00:09:18,850 --> 00:09:24,410
+4 ل 6 أسابيع تقريبا كل البكتيريا بتحتاج 24 ساعة
+
+131
+00:09:24,410 --> 00:09:28,070
+فقط حتى تنمو لكن الـ Mycobacterium tuberculosis
+
+132
+00:09:28,070 --> 00:09:33,280
+بتحتاج من 4 ل 6 أسابيع حتى تنمونتيجة وجود الـ wax
+
+133
+00:09:33,280 --> 00:09:37,480
+lipid الـ wax lipid بيعمل كحاجز ببطء من وصول
+
+134
+00:09:37,480 --> 00:09:42,720
+المغذيات للبكتيريا فبتاخد وقت أطول حتى تنمو عشان
+
+135
+00:09:42,720 --> 00:09:48,360
+هيك الدكتور بيطلب فحص ال acid fast stand كفحص سريع
+
+136
+00:09:48,360 --> 00:09:52,820
+رابد مش هيستنى 6 أسابيع يستنى نمو البكتيريا خلال
+
+137
+00:09:52,820 --> 00:09:57,740
+نص ساعة بتطلع نتيجة ال acid fast stand preliminary
+
+138
+00:09:57,740 --> 00:10:03,780
+diagnosis of tuberculosis هو فحصأولي لأنه لازم أنا
+
+139
+00:10:03,780 --> 00:10:07,840
+بعدين أعمل مزرعة أزرع البكتيريا و تنمو و أعمل
+
+140
+00:10:07,840 --> 00:10:11,260
+فحوصات كيميائية عشان أتأكد أنه هي Mycobacterium
+
+141
+00:10:11,260 --> 00:10:15,740
+tuberculosis و the greater than 90% productive
+
+142
+00:10:15,740 --> 00:10:19,880
+value from sputum sample بقولّي أنه لو انت عملتي
+
+143
+00:10:19,880 --> 00:10:24,780
+ال acid fastest أنه طلعت acid fast بسلعي 90% بنحكي
+
+144
+00:10:24,780 --> 00:10:28,500
+أنه هي البكتيريا Mycobacterium tuberculosis طب ليش
+
+145
+00:10:28,500 --> 00:10:32,870
+مش 100%؟لأنه لازم نزرع البكتيريا ونشوف نمو
+
+146
+00:10:32,870 --> 00:10:36,690
+البكتيريا على الطبق ونعمل فحوصات نتأكد أن هي
+
+147
+00:10:36,690 --> 00:10:41,770
+Mycobacterium tuberculosis هيك بأحكائنه نسبة 100%
+
+148
+00:10:41,770 --> 00:10:46,050
+وحتى نتيجة الـ Acid fast stand ما بنكتب النتيجة ال
+
+149
+00:10:46,050 --> 00:10:49,030
+positive لو كانت بصل لاي ألونها أحمر ما بكتب
+
+150
+00:10:49,030 --> 00:10:54,130
+Mycobacterium غلط بنكتب Acid fast بصل لاي بصل لاي
+
+151
+00:10:54,130 --> 00:10:58,640
+لأن Mycobacterium tuberculosis شكلها بصل لايالـ
+
+152
+00:10:58,640 --> 00:11:03,140
+Acid Fast معناها أنها بتقاوم الحمض الحمض اللي هو 3
+
+153
+00:11:03,140 --> 00:11:10,360
+% HCl الـ Decolorizer عشان هي احتفظت باللون لون
+
+154
+00:11:10,360 --> 00:11:13,820
+الـ Carb والفوكسين لبريمارستين وماقدر حتى الحمض
+
+155
+00:11:13,820 --> 00:11:18,420
+القوي الـ HCl يزيل هذا اللون طبعا هذه الخاصية أنها
+
+156
+00:11:18,420 --> 00:11:22,020
+بتقاوم الحمض خاصية من خصائص الـ Mycobacterium
+
+157
+00:11:22,020 --> 00:11:25,660
+tuberculosis من خلال هذه الخاصية قدرت أتعرف على
+
+158
+00:11:25,660 --> 00:11:30,930
+البكتيرياكيف انا بدي اكتب نتيجة الـacid fast stain
+
+159
+00:11:30,930 --> 00:11:34,610
+من خلال هذا الجدول هنتعرف بقولك لو فحصتي في ال
+
+160
+00:11:34,610 --> 00:11:39,690
+field وماجفتي اي acid fast بصلاي بصلاي شكلها بصلاي
+
+161
+00:11:39,690 --> 00:11:44,410
+acid fast يعني بتاخد اللون الأحمر بتكتبي نتيجة no
+
+162
+00:11:44,410 --> 00:11:48,710
+acid fast stain ممنوع تكتبيه negative لأنه ممكن
+
+163
+00:11:48,710 --> 00:11:54,290
+يكون شغلك غلط ممكن طريقة جمعك للعينة غلط تكتبي no
+
+164
+00:11:54,290 --> 00:11:58,870
+acid fast stain معناههذه الجملة إنك انت فحصت بال
+
+165
+00:11:58,870 --> 00:12:03,910
+field وما شفت أي بكتيريا لو فحصت 300 field وكانت
+
+166
+00:12:03,910 --> 00:12:09,150
+البكتيريا من 1 ل 2 acid-fast-bucyllite بتكون
+
+167
+00:12:09,150 --> 00:12:13,270
+العينة دبط full مشكوك فيها بقولك عيدي الفحص ال
+
+168
+00:12:13,270 --> 00:12:17,430
+acid-fast-stain marker تأتي لو فحصت 100 field وكان
+
+169
+00:12:17,430 --> 00:12:22,740
+من 1 ل 9 acid-fast-bucyllite تعطي plus oneلو عشر
+
+170
+00:12:22,740 --> 00:12:25,840
+في الـ data حصلت وكان acid fast بصلاي من واحد
+
+171
+00:12:25,840 --> 00:12:29,620
+لتسعة plus two لو feed واحد كان فيه من واحد لتسعة
+
+172
+00:12:29,620 --> 00:12:33,120
+acid fast بصلاي plus three لو ال feed الواحد كان
+
+173
+00:12:33,120 --> 00:12:39,180
+أكتر من تسعة بتكون plus four بقولنا it also can be
+
+174
+00:12:39,180 --> 00:12:42,540
+performed in patients able to track the progress
+
+175
+00:12:42,540 --> 00:12:46,440
+of antibiotic therapy بقولنا أن ال acid fast 10
+
+176
+00:12:46,440 --> 00:12:50,840
+ممكن من خلالها أشوف استجابة المريض للعلاج على سبيل
+
+177
+00:12:50,840 --> 00:12:55,760
+المثالالمريض كان plus three والدكتور وصف له علاج
+
+178
+00:12:55,760 --> 00:13:01,340
+بعد مدة معينة طلب الفحص مرة تانية لازم حتى أن
+
+179
+00:13:01,340 --> 00:13:05,960
+المريض يكون استجاب للعلاج لازم يكون plus two أو
+
+180
+00:13:05,960 --> 00:13:10,440
+plus one and determine their degree of
+
+181
+00:13:10,440 --> 00:13:14,430
+contagiousnessماذا يعني كونتاجستنس؟ كونتاجستنس
+
+182
+00:13:14,430 --> 00:13:19,410
+معناها شراسة المرض أكيد الـPlus-4 هتكون أكتر شراسة
+
+183
+00:13:19,410 --> 00:13:23,430
+من الـPlus-2 يبقى من خلال الـGram أو من خلال
+
+184
+00:13:23,430 --> 00:13:28,130
+الـAcid-fast-strain بقدر أتعرف على قوة شراسة المرض
+
+185
+00:13:28,130 --> 00:13:34,630
+Acid
+
+186
+00:13:34,630 --> 00:13:39,840
+-fast-reagent أول reagent للـCarbوفكسينتعطي اللون
+
+187
+00:13:39,840 --> 00:13:43,720
+الـ Red a phenolic stain لأنه أحد مكونات الصبغة
+
+188
+00:13:43,720 --> 00:13:47,380
+الـ phenol is the primary stain in the acid fast
+
+189
+00:13:47,380 --> 00:13:51,280
+test هو الصبغة الأولية اللي بنستخدمها في ال acid
+
+190
+00:13:51,280 --> 00:13:55,340
+fast test it is soluble in the lipid of the
+
+191
+00:13:55,340 --> 00:13:59,580
+mycobacterial cell wall بقولنا هذه الصبغة بتدوب في
+
+192
+00:13:59,580 --> 00:14:02,900
+الدهون الموجودة على ال cell wall تبع ال
+
+193
+00:14:02,900 --> 00:14:07,020
+mycobacterium tuberculosis يبقى هي lipid soluble
+
+194
+00:14:07,310 --> 00:14:11,230
+هذا هيساعد في اختراق الصبغة الـ Carbol Fuxin
+
+195
+00:14:11,230 --> 00:14:15,610
+للواكس لبن it is consists of basic Fuxin بقولك
+
+196
+00:14:15,610 --> 00:14:19,450
+مكونات هذه الصبغة basic Fuxin هو اللي بيعطي اللون
+
+197
+00:14:19,450 --> 00:14:24,450
+on carbolic acid الفينول الفينول يعتبر as mordant
+
+198
+00:14:24,450 --> 00:14:29,090
+مع ال cooling هيثبت ال Carbol Fuxin داخل البكتيريا
+
+199
+00:14:29,090 --> 00:14:33,140
+heating the specimenبقولنا بتم تسخين العينة
+
+200
+00:14:33,140 --> 00:14:35,860
+وتعريضها للـ steam عشان يزيد من اختراق
+
+201
+00:14:35,860 --> 00:14:39,240
+الكربوفوكسين للـ wax lipid طب لو أنا ما بنتي
+
+202
+00:14:39,240 --> 00:14:42,640
+أعرضها للـ steam بقولك ممكن أضيفي wetting agent
+
+203
+00:14:42,640 --> 00:14:47,760
+such as tergatol ممكن أنضيف tergatol هيعمل نفس عمل
+
+204
+00:14:47,760 --> 00:14:50,640
+لـ steam increase the penetration of the
+
+205
+00:14:50,640 --> 00:14:54,460
+kerbofoxin هيزيد من اختراق الصبقة للـ wax lipid
+
+206
+00:14:54,460 --> 00:14:58,040
+بجيب slide و بحط drop of tergatol و ما بحطها على
+
+207
+00:14:58,040 --> 00:15:07,590
+إلاهابعد مضيف الكربوفوكسين العينة منبردها وهذا
+
+208
+00:15:07,590 --> 00:15:11,690
+بيكون as mordant بعدها بدي أضيف الـ decolorizer
+
+209
+00:15:11,690 --> 00:15:17,490
+اللي هو أسد ألكحول مش ألكحول لحال الأسد 3% HCl
+
+210
+00:15:17,490 --> 00:15:21,030
+والألكحول 95% Ethanol
+
+211
+00:15:25,760 --> 00:15:28,880
+بالرغم من أن الكربوفوكسين أكثر مستعملable في الوكس
+
+212
+00:15:28,880 --> 00:15:33,620
+الليبد من في الاسد الكحول ذوبانية الكربوفوكسين في
+
+213
+00:15:33,620 --> 00:15:37,280
+الوكس الليبد أعلى من ذوبانيتها في الاسد الكحول
+
+214
+00:15:37,280 --> 00:15:41,760
+الاسد الكحول يزيل الكربوفوكسين من نوع من الأجسام
+
+215
+00:15:41,760 --> 00:15:42,340
+الليبدية من نوع من الأجسام الليبدية من نوع من
+
+216
+00:15:42,340 --> 00:15:45,860
+الأجسام الليبدية من نوع من الأجسام الليبدية من نوع
+
+217
+00:15:45,860 --> 00:15:54,980
+من الأجسام الليبدية من نوع من الأجسام الليبديةلكن
+
+218
+00:15:54,980 --> 00:15:58,080
+ماقدر يزيل اللون من الـ Acid Phospholipid-Bacillus
+
+219
+00:15:58,080 --> 00:16:00,260
+الـ Acid Phospholipid-Bacillus زي الـ
+
+220
+00:16:00,260 --> 00:16:03,580
+Mycobacterium tuberculosis بتحتوي على واكسي لبت
+
+221
+00:16:03,580 --> 00:16:07,880
+الكاربولفوكسين هتدوب في هذه الواكسي لبت بعدها هضيف
+
+222
+00:16:07,880 --> 00:16:11,940
+الـ Decolorizer اللي هو الاسد الكحول فمش هيقدر
+
+223
+00:16:11,940 --> 00:16:15,640
+يزيل اللون منها اللون صبغة الكاربولفوكسين لأن
+
+224
+00:16:15,640 --> 00:16:20,740
+الكاربولفوكسين زابت بالاصل في الواكسي لبتفمش هقدر
+
+225
+00:16:20,740 --> 00:16:24,040
+أن أفصل الـ Carbolfoxin عن الـ Wax Lipid زي السكر
+
+226
+00:16:24,040 --> 00:16:27,160
+لما يدوب في الميه مابقدر أفصلهم عن بعض فلما يجي ال
+
+227
+00:16:27,160 --> 00:16:31,740
+Decalarizer بعدها هيكون ال Carbofoxin دائم في ال
+
+228
+00:16:31,740 --> 00:16:36,100
+Wax Lipid مش هيقدر يزيل اللون لكن الـ Non-acid
+
+229
+00:16:36,100 --> 00:16:40,540
+fast organism ما بتحتوي على Wax Lipid فأول إشي
+
+230
+00:16:40,540 --> 00:16:44,180
+هصبغها ب Primers تان ل Carbofoxin هتاخد الصبغة بكل
+
+231
+00:16:44,180 --> 00:16:47,960
+سهولة بعدها هنضيف ال Decalarizer ال Acid Alcohol
+
+232
+00:16:48,430 --> 00:16:52,190
+الـ Carbolfoxin هيدوب في الـ acid alcohol لأنه أنا
+
+233
+00:16:52,190 --> 00:16:55,890
+ماعندي wax lipid فاللون هيروح بكل سهولة و هتصير
+
+234
+00:16:55,890 --> 00:16:59,850
+شفّع فهضطر أصبغها بـ counter stain with methylene
+
+235
+00:16:59,850 --> 00:17:02,650
+blue الـ methylene blue هي ال counter stain which
+
+236
+00:17:02,650 --> 00:17:05,850
+cannot penetrate mycolic acid مابتقدر تخترق ال
+
+237
+00:17:05,850 --> 00:17:08,730
+mycolic acid ال waxy lipid لأنه هي مش lipid
+
+238
+00:17:08,730 --> 00:17:13,030
+soluble زي ال Carbolfoxin وماعرضت الصبغة لبخار
+
+239
+00:17:13,030 --> 00:17:17,010
+provide contrast to non acid fast cell بقولك هذا
+
+240
+00:17:18,070 --> 00:17:23,790
+بميزها و هي بتعطي اللون لـ non acid fast cell و
+
+241
+00:17:23,790 --> 00:17:27,390
+بقدر انا افرقها بين ال acid fast بس ال lie و non
+
+242
+00:17:27,390 --> 00:17:31,850
+acid fast بس ال lie Mycobacteria are difficult to
+
+243
+00:17:31,850 --> 00:17:35,970
+stain ال mycobacteria صعب صباغتها but once stained
+
+244
+00:17:35,970 --> 00:17:43,000
+لو انا قدرت أصبغها زي ما قدرنا في المعملصعب إزالة
+
+245
+00:17:43,000 --> 00:17:47,820
+اللون منها حتى في وجود
+
+246
+00:17:47,820 --> 00:17:53,460
+حمض قوي صعب إن أحنا نزيل اللون من الميكوبكتيريوم
+
+247
+00:17:53,460 --> 00:17:58,120
+وهي طبعا خاصية أو ميزة من خلالها قدرت أتعرف على
+
+248
+00:17:58,120 --> 00:18:03,000
+البكتيريوم بالنسبة
+
+249
+00:18:03,000 --> 00:18:06,180
+للخطوات Smelly preparation, air-drying, heat
+
+250
+00:18:06,180 --> 00:18:11,240
+-fixation البراميرستان الكربوفكسينحتى انه تساعد
+
+251
+00:18:11,240 --> 00:18:15,680
+اختراق الكربوفوكسين للوقس اللي بضيف تيرجيتول لمدة
+
+252
+00:18:15,680 --> 00:18:20,560
+خمس دقائق لو ماعندي تيرجيتول بغرد ال slide ل ال
+
+253
+00:18:20,560 --> 00:18:26,120
+steam لمدة تمان دقائق بعدها بعمل cooling تبريد ل
+
+254
+00:18:26,120 --> 00:18:31,380
+ال slide بكون as more than اكسد الكحول اللي هو ال
+
+255
+00:18:31,380 --> 00:18:35,340
+decolorizer لمدة خمس عشر لعشرين second بعدها بغسل
+
+256
+00:18:35,340 --> 00:18:39,650
+بال water بضيف ال counterاللي نقبله لمدة one
+
+257
+00:18:39,650 --> 00:18:43,250
+minute او تلاتين second بغسل بال tap water air
+
+258
+00:18:43,250 --> 00:18:46,050
+drying و بشوفها تحت ال microscope على ال oil
+
+259
+00:18:46,050 --> 00:18:49,910
+immersion positive result a pair pink or red بتكون
+
+260
+00:18:49,910 --> 00:18:54,190
+لازم تكون bustle line negative organism بتظهر كلون
+
+261
+00:18:54,190 --> 00:18:56,590
+أزرق كوكاي أو bustle line
+
+262
+00:19:00,870 --> 00:19:05,210
+قبل ال-staining البكتيريا بتكون transparent صبغت
+
+263
+00:19:05,210 --> 00:19:09,310
+بي الكاربولفوكسين بالـprimary stain سواء كانت acid
+
+264
+00:19:09,310 --> 00:19:13,490
+-fast-bacilli أو non-acid-fast-bacilli هتاخد اللون
+
+265
+00:19:13,490 --> 00:19:16,790
+الـred بعدها ضفت الـdecolorizer اللي هو الاسد
+
+266
+00:19:16,790 --> 00:19:21,430
+الكحول هينزل اللون من الـnon-acid-fast-bacilli لأن
+
+267
+00:19:21,430 --> 00:19:25,940
+سرعة ذوبانية الكاربولفوكسينفي الـ wax lipid أعلى
+
+268
+00:19:25,940 --> 00:19:29,540
+من الاسد الكحول بالنسبة لـ Non-acid fast-bust lima
+
+269
+00:19:29,540 --> 00:19:33,300
+بتحتوي على wax lipid فبالتالي الكربوفوكسين هيدوب
+
+270
+00:19:33,300 --> 00:19:37,600
+في الاسد الكحول و اللون هيروح لكن الاسد fast-bust
+
+271
+00:19:37,600 --> 00:19:42,080
+lime البكتيريا بتحتوي على wax lipid الكربوفوكسين
+
+272
+00:19:42,080 --> 00:19:45,660
+هيدوب في ال wax lipid هييجي ال decolorizer مش
+
+273
+00:19:45,660 --> 00:19:49,260
+هيقدر يزيل اللون بعدها هضيف ال counterstain
+
+274
+00:19:49,260 --> 00:19:54,040
+الميثيلين blue أكيد البكتيريا الليكانت transparent
+
+275
+00:19:54,040 --> 00:19:57,640
+شفافة فهتاخد اللون اللي هو اللون الأزرق لكن
+
+276
+00:19:57,640 --> 00:20:01,280
+البكتيريا ال acid fast basalite هتظلها محتفظة
+
+277
+00:20:01,280 --> 00:20:05,780
+باللون ال red لأنه احنا قولنا انه هي صعب ازالة
+
+278
+00:20:05,780 --> 00:20:10,500
+اللون منها فبالتالي هتظلها red و basalite لكن ال
+
+279
+00:20:10,500 --> 00:20:16,200
+non acid fast organism ممكن تكون كوكاي او basalite
+
+280
+00:20:16,200 --> 00:20:18,460
+لكن اللون، اللون blue
+
+281
+00:20:22,120 --> 00:20:26,440
+هي نتائج acid fast stain منلاحظ كل الصور هي acid
+
+282
+00:20:26,440 --> 00:20:31,220
+fast بصلاي هيك بكتب النتيجة acid fast بصلاي بصلاي
+
+283
+00:20:31,220 --> 00:20:36,040
+الشعب تبعها بصلاي عصوي و acid fast يعني قاومة
+
+284
+00:20:36,040 --> 00:20:41,900
+الحمض 3% FCL decolorizer احتفظت بلون ال primer
+
+285
+00:20:41,900 --> 00:20:46,720
+stain الكربوفوكسينومائد حتى الحمض القوي يزيل اللون
+
+286
+00:20:46,720 --> 00:20:47,180
+منها
+
+287
+00:20:51,330 --> 00:20:54,970
+Cannon Stain or cold method method of staining
+
+288
+00:20:54,970 --> 00:20:58,970
+acid fast microorganism هي طريقة لصباغة ال acid
+
+289
+00:20:58,970 --> 00:21:03,350
+fast بسلعي خاصة الميكوبكتيريا procedure is similar
+
+290
+00:21:03,350 --> 00:21:07,470
+to zel nelson stain الخطوات هي نفس خطوات ال zel
+
+291
+00:21:07,470 --> 00:21:10,850
+nelson stain اللي هي ال acid fast stain but
+
+292
+00:21:10,850 --> 00:21:14,610
+doesn't involve heating the slide لكن أنا ما بعرض
+
+293
+00:21:14,610 --> 00:21:17,770
+ال slide ل steam عشان هيك سميتها بال cold method
+
+294
+00:21:18,400 --> 00:21:22,440
+Kynon Staining method used carboxyne as a primary
+
+295
+00:21:22,440 --> 00:21:26,000
+stain استخدم الكربوفوكسين ك primary stain أسود
+
+296
+00:21:26,000 --> 00:21:29,820
+ألكوهول ك decolorizer والميتاليني بلو ك counter
+
+297
+00:21:29,820 --> 00:21:34,080
+stain طيب شو الفرق؟ الكنون كربوفوكسين has a
+
+298
+00:21:34,080 --> 00:21:37,340
+greater concentration of phenol and basicfoxine
+
+299
+00:21:37,340 --> 00:21:41,760
+بقولنا إنه فيه اندي تركيز عالي من الفينول ومن ال
+
+300
+00:21:41,760 --> 00:21:45,760
+basicfoxine اللي هم أحد مكونات الكربوفوكسين
+
+301
+00:21:45,760 --> 00:21:50,980
+فبالتاليما بحتاج الـ heat الحرارة حتى إنها تخترق
+
+302
+00:21:50,980 --> 00:21:55,760
+الـ waxy label تحت المايكروسكوب بشوف الخلايا الـ
+
+303
+00:21:55,760 --> 00:21:58,680
+acid-fast organism بتظهر باللون الأحمر و الـ non
+
+304
+00:21:58,680 --> 00:22:03,340
+-acid-fast organism بتظهر باللون الأزرق نفس نتائج
+
+305
+00:22:03,340 --> 00:22:09,940
+الـ acid-fastستانورامينرودامينستان
+
+306
+00:22:10,310 --> 00:22:13,670
+Oramine rhodamine fluorochrome staining هي الطريقة
+
+307
+00:22:13,670 --> 00:22:17,130
+التالتة حتى أني أنا أقدر أشوف ال acid fast-bacilli
+
+308
+00:22:17,130 --> 00:22:20,650
+اللي هي ال mycobacterium الطريقة الأولى كانت ال
+
+309
+00:22:20,650 --> 00:22:23,470
+acid fast-stain أو ال Zellnelson stain الطريقة
+
+310
+00:22:23,470 --> 00:22:26,290
+التانية كانت ال quinone stain و الطريقة التالتة
+
+311
+00:22:26,290 --> 00:22:30,030
+اللي هي هاي ال oramine rhodamine stain من خلال هاي
+
+312
+00:22:30,030 --> 00:22:34,090
+الطريقة بقدر أشوف ال acid fast-bacilli باستخدام ال
+
+313
+00:22:34,090 --> 00:22:37,650
+fluorescent microscope هتظهر ال mycobacterium بلون
+
+314
+00:22:37,650 --> 00:22:39,150
+أصفر reddish yellow
+
+315
+00:22:42,740 --> 00:22:46,980
+أخر معلومة في الـ Acid Fast StainThe reddish pink
+
+316
+00:22:46,980 --> 00:22:50,720
+color and carding sticking together in long ruby
+
+317
+00:22:50,720 --> 00:22:54,320
+masses of the mycobacterium cell due to the excess
+
+318
+00:22:54,320 --> 00:22:57,240
+lipid of the cell wall بقولنا في انديانا طبقة
+
+319
+00:22:57,240 --> 00:23:01,280
+شمعية بتخلي البكتيريا تلزق بعض بتكون مبينة على شكل
+
+320
+00:23:01,280 --> 00:23:05,780
+الحبل carding تمام هذه الظاهرة اسمها الـ ruby
+
+321
+00:23:05,780 --> 00:23:10,730
+masses أو ممكن نسميها cardingهذه الظاهرة تكون في
+
+322
+00:23:10,730 --> 00:23:14,790
+الـ Mycobacterium نتيجة الـ high concentration of
+
+323
+00:23:14,790 --> 00:23:15,410
+lipid
+
diff --git a/PL9fwy3NUQKwZWgAb_V1ATlRRsH-6i3cPC/9AIy0KlGCSw.srt b/PL9fwy3NUQKwZWgAb_V1ATlRRsH-6i3cPC/9AIy0KlGCSw.srt
new file mode 100644
index 0000000000000000000000000000000000000000..d9996d919074f6f91324bc52c860a4a3efc4a5b3
--- /dev/null
+++ b/PL9fwy3NUQKwZWgAb_V1ATlRRsH-6i3cPC/9AIy0KlGCSw.srt
@@ -0,0 +1,1374 @@
+
+1
+00:00:00,720 --> 00:00:04,220
+السلام عليكم ورحمة الله وبركاته، يعطيكم العافية
+
+2
+00:00:04,220 --> 00:00:07,780
+جميعًا في هذا اللقاء، سنتحدث عن bacterial
+
+3
+00:00:07,780 --> 00:00:12,700
+motility، حركة البكتيريا. البكتيريا تتحرك بواسطة
+
+4
+00:00:12,700 --> 00:00:16,060
+الفلاجلة، وعرفنا أن الفلاجلة يعتبر special
+
+5
+00:00:16,060 --> 00:00:19,360
+component، يعني في بكتيريا عندي motile وفي بكتيريا
+
+6
+00:00:19,360 --> 00:00:24,120
+non-motile.  عشان أعرف هل البكتيريا متحركة أم لا، في
+
+7
+00:00:24,120 --> 00:00:28,940
+عندي ثلاث طرق سنتعرف عليها في هذه المحاضرة. أحد هذه
+
+8
+00:00:28,940 --> 00:00:33,300
+الطرق هو Flagella Staining، الذي يعتبر special
+
+9
+00:00:33,300 --> 00:00:38,540
+stain. لو بدنا نعرف motility، Motility: the
+
+10
+00:00:38,540 --> 00:00:42,780
+ability of an organism to move by itself، هو قدرة
+
+11
+00:00:42,780 --> 00:00:47,040
+الكائن الحي أو البكتيريا على الحركة بنفسها
+
+12
+00:00:47,040 --> 00:00:52,640
+by itself. هذا هو تعريف motility، قدرة الكائن الحي
+
+13
+00:00:52,640 --> 00:00:55,660
+على الحركة بنفسها by itself.
+
+14
+00:01:00,530 --> 00:01:05,030
+لماذا by itself؟ لأنه ممكن تتحرك by activity of water
+
+15
+00:01:05,030 --> 00:01:09,070
+molecules، بواسطة حركة جزيئات الماء، وهي تعتبر
+
+16
+00:01:09,070 --> 00:01:13,290
+Brownian movement. لكن لو تحركت البكتيريا by
+
+17
+00:01:13,290 --> 00:01:18,170
+itself، أسميها True movement. Motility is closely
+
+18
+00:01:18,170 --> 00:01:21,810
+linked with chemotaxis. مصطلح motility مرتبط
+
+19
+00:01:21,810 --> 00:01:26,190
+بمصطلح آخر اسمه chemotaxis. لو بدنا نعرف chemotaxis: the
+
+20
+00:01:26,190 --> 00:01:30,310
+ability to move oriented along certain
+
+21
+00:01:30,310 --> 00:01:34,910
+chemical gradients، هي حركة البكتيريا حسب تركيز
+
+22
+00:01:34,910 --> 00:01:38,850
+المواد الكيميائية. لو كان في عندي مغذيات، أكيد
+
+23
+00:01:38,850 --> 00:01:43,150
+ستتحرك البكتيريا باتجاه هذه المغذيات. لو كان في
+
+24
+00:01:43,150 --> 00:01:47,990
+عندي مواد سامة (toxic)، ستتحرك بعيدًا عن المواد السامة، ستهرب منها. A
+
+25
+00:01:47,990 --> 00:01:51,590
+large number of bacteria are motile. أعداد كبيرة
+
+26
+00:01:51,590 --> 00:01:56,070
+من البكتيريا تعتبر motile، يبقى النسبة القليلة
+
+27
+00:01:56,070 --> 00:02:00,330
+تعتبر non-motile. Most possess one or more flagella
+
+28
+00:02:00,330 --> 00:02:04,310
+on their surface that allow them to swim. البكتيريا
+
+29
+00:02:04,310 --> 00:02:08,790
+المتحركة تتحرك بالفلاجلة (الأَسْواط). تمتلك إما سوطًا
+
+30
+00:02:08,790 --> 00:02:13,630
+واحدًا أو مجموعة أسواط على السطح، يسمح لها بالحركة.
+
+31
+00:02:14,290 --> 00:02:17,990
+بكتيريا والفلاجلة. لو بدنا نعرف الفلاجلة: are tiny
+
+32
+00:02:17,990 --> 00:02:22,810
+hair-like organelles of locomotion، هي عبارة عن
+
+33
+00:02:22,810 --> 00:02:28,970
+أَسْواط دقيقة كاللشَعْر، تستخدم في الحركة. من أين ينشأ
+
+34
+00:02:28,970 --> 00:02:33,190
+الفلاجلة؟ originating in the cytoplasm، ينشأ من
+
+35
+00:02:33,190 --> 00:02:38,930
+السيتوبلازم (cytoplasm) أسفل جدار الخلية (beneath the cell wall). يَخْرُج السوط خارج
+
+36
+00:02:38,930 --> 00:02:43,830
+جدار الخلية (cell wall). they extend beyond the cell، ويمتد خارج
+
+37
+00:02:43,830 --> 00:02:48,910
+الخلية. أقول: طول السوط قد يساوي طول الخلية
+
+38
+00:02:48,910 --> 00:02:53,890
+البكتيرية أو أطول منها بقليل. their fine protein
+
+39
+00:02:53,890 --> 00:02:57,850
+structure. عن أي بروتين يقصد في هذه الشريحة؟
+
+40
+00:02:57,850 --> 00:03:05,090
+أقول إن الفلاجلة تتكون من بروتين أسميه الفلاجلين.
+
+41
+00:03:05,090 --> 00:03:09,370
+هذا البروتين يعتبر fine، خفيف، لا أقدر أن أراه
+
+42
+00:03:09,370 --> 00:03:13,250
+وبالتالي، يحتاج إلى  (require) Specialist Staining
+
+43
+00:03:13,250 --> 00:03:17,710
+Technique، يحتاج إلى صبغات خاصة، for demonstrating
+
+44
+00:03:17,710 --> 00:03:21,410
+them with a light microscope،  عشان أقدر أراه تحت
+
+45
+00:03:21,410 --> 00:03:24,690
+المجهر. يبقى البروتين المقصود في هذه
+
+46
+00:03:24,690 --> 00:03:29,370
+الجملة هو الفلاجلين، لأن الفلاجلة تتكون من
+
+47
+00:03:29,370 --> 00:03:31,150
+البروتين اسمه الفلاجلين.
+
+48
+00:03:36,770 --> 00:03:41,210
+موقع الأسواط (site of flagella). نمط
+
+49
+00:03:41,210 --> 00:03:44,370
+الأسواط (pattern of flagellation) هو سمة مهمة في
+
+50
+00:03:44,370 --> 00:03:48,550
+التعريف (identification) للبكتيريا المتحركة. وتوزيع الأسواط
+
+51
+00:03:48,550 --> 00:03:52,890
+للبكتيريا المتحركة مهم جدًا في التعريف، كما
+
+52
+00:03:52,890 --> 00:03:55,790
+قلنا في Indus Bar location، أنه يساعد في
+
+53
+00:03:55,790 --> 00:03:58,950
+تعريف البكتيريا. كمان، موقع الأسواط يساعد
+
+54
+00:03:58,950 --> 00:04:03,370
+في التعريف. لو كانت البكتيريا is a single
+
+55
+00:04:03,370 --> 00:04:08,280
+polar flagellum، أسمي البكتيريا monotrichous. لو
+
+56
+00:04:08,280 --> 00:04:13,260
+كانت تمتلك سوطًا واحدًا فقط، أكيد سيكون على قطب واحد.
+
+57
+00:04:13,260 --> 00:04:21,200
+أسمي البكتيريا monotrichous. amphitrichous: one or
+
+58
+00:04:21,200 --> 00:04:25,440
+more at both poles. لو كان في عندي سوط
+
+59
+00:04:25,440 --> 00:04:32,600
+واحد، لكن على كلا القطبين، سوط واحد على كلا القطبين،
+
+60
+00:04:32,600 --> 00:04:36,640
+أسميه amphitrichous. أو مجموعة أسواط، ممكن يكون
+
+61
+00:04:36,640 --> 00:04:41,340
+مجموعة أسواط على كلا القطبين، أسميه
+
+62
+00:04:41,340 --> 00:04:45,660
+amphitrichous. lophotrichous: tufts of flagella
+
+63
+00:04:45,660 --> 00:04:49,440
+at one end pole. لو كان في عندي حزمة (tufts)، يعني
+
+64
+00:04:49,440 --> 00:04:54,040
+حزمة من الأسواط (of flagella) at one end pole. لو كان في عندي
+
+65
+00:04:54,040 --> 00:04:59,180
+حزمة من الأسواط موجودة على قطب واحد فقط، أسميها lophotrichous.
+
+66
+00:04:59,180 --> 00:05:03,090
+كذلك، فقط على قطب واحد،  مش زي amphitrichous، على القطبين.
+
+67
+00:05:03,090 --> 00:05:07,330
+monotrichous: سوط على
+
+68
+00:05:07,330 --> 00:05:12,350
+قطب، لكن lophotrichous: مجموعة أسواط على قطب
+
+69
+00:05:12,350 --> 00:05:17,050
+واحد. peritrichous: لو كانت الأسواط تحيط بالخلية
+
+70
+00:05:17,050 --> 00:05:21,190
+البكتيرية، زي ما إحنا شايفين في الصورة، هذه الأسواط
+
+71
+00:05:21,190 --> 00:05:26,770
+تحيط بالخلية البكتيرية، أسميها peritrichous. طيب،
+
+72
+00:05:26,770 --> 00:05:30,780
+trichous يعني "شعر"،  non-motile يعني لا
+
+73
+00:05:30,780 --> 00:05:34,460
+تتحرك. without flagella، أكيد لن يكون عندها أسواط.
+
+74
+00:05:34,460 --> 00:05:38,800
+لا تجذبها أي أسواط. هذا بالنسبة لموقع
+
+75
+00:05:38,800 --> 00:05:42,820
+الأسواط. قلنا إن موقع الأسواط يساعد
+
+76
+00:05:42,820 --> 00:05:46,320
+في التعريف كيف؟ لو كان عندي، أنا عملت
+
+77
+00:05:46,320 --> 00:05:50,200
+اختبارًا جرثوميًا، وظهرت النتيجة Gram-negative bacilli،
+
+78
+00:05:50,200 --> 00:05:53,700
+أكيد Gram-negative bacilli تضم مجموعة كبيرة من
+
+79
+00:05:53,700 --> 00:05:57,740
+البكتيريا. عملت مجموعة فحوصات، في النهاية، وصلت إلى أن
+
+80
+00:05:58,000 --> 00:06:03,680
+البكتيريا إما X أو Y، كانت X و Y متحركتان (motile)، لكن توزيع
+
+81
+00:06:03,680 --> 00:06:07,640
+الأسواط لهما مختلف. من خلال موقع الأسواط
+
+82
+00:06:07,640 --> 00:06:17,440
+(site of the flagella)، أقدر أعمل تعريفًا (identification). Most
+
+83
+00:06:17,440 --> 00:06:21,640
+motile bacteria move in a straight line for a
+
+84
+00:06:21,640 --> 00:06:25,240
+brief time. أقول: معظم البكتيريا المتحركة تتحرك
+
+85
+00:06:25,240 --> 00:06:30,290
+في خط مستقيم لفترة وجيزة، then turn and randomly
+
+86
+00:06:30,290 --> 00:06:33,870
+change direction before swimming again. أقول: بعدها
+
+87
+00:06:33,870 --> 00:06:38,470
+تغير الاتجاه، وتتحرك بشكل عشوائي. الفلاجلة
+
+88
+00:06:38,470 --> 00:06:42,530
+composed from، أقول: من ماذا تتكون الفلاجلة؟ تتكون
+
+89
+00:06:42,530 --> 00:06:46,970
+من خيوط (filament) composed of a protein called flagellin.
+
+90
+00:06:46,970 --> 00:06:52,380
+قلنا على الفلاجلين (flagellin). تتكون الفلاجلة من خيوط (filament).
+
+91
+00:06:52,380 --> 00:06:56,400
+هذه الخيوط (filament) تتكون من البروتين الفلاجلين. يبقى
+
+92
+00:06:56,400 --> 00:07:01,880
+الفلاجلين (flagellin): a protein that composes the filament. الخطاف (hook)،
+
+93
+00:07:01,880 --> 00:07:05,400
+قاعدة الخيط (base of filament) بالقرب من جدار الخلية (near the cell wall). هو
+
+94
+00:07:05,400 --> 00:07:10,360
+قاعدة الخيط، تكون قريبة من جدار الخلية.  الجزء الأساسي (basal body)،
+
+95
+00:07:10,360 --> 00:07:14,240
+الجزء الأخير، مكون من البروتينات الأساسية، يقول لي: يربط،
+
+96
+00:07:14,240 --> 00:07:18,040
+يربط أو يدعم الخيط (filament) والخطاف (hook) بجدار الخلية (to the cell
+
+97
+00:07:18,040 --> 00:07:22,600
+wall). يربط ويدعم الخيط والخطاف بجدار الخلية.
+
+98
+00:07:22,600 --> 00:07:28,880
+في عندي رسمة: الخيط (filament) الذي قلنا إنه يتكون
+
+99
+00:07:28,880 --> 00:07:32,580
+من بروتين الفلاجلين، في عندي الخطاف (hook)، هو
+
+100
+00:07:32,580 --> 00:07:36,920
+قاعدة الخيط (filament)، وفي عندي البروتينات الأساسية (basal body) التي تربط
+
+101
+00:07:36,920 --> 00:07:42,460
+الخطاف والخيط بجدار الخلية. type of
+
+102
+00:07:42,460 --> 00:07:47,840
+movement. ممكن تكون عندي run، حركة خط مستقيم (run: straight line
+
+103
+00:07:47,840 --> 00:07:50,780
+movement). occur when the flagella rotate counter
+
+104
+00:07:50,780 --> 00:07:54,720
+clockwise. البكتيريا تتحرك بنوعين من الحركة، إما
+
+105
+00:07:54,720 --> 00:08:00,740
+run، تتحرك عكس عقارب الساعة، tumble، التي هي turning
+
+106
+00:08:00,740 --> 00:08:03,960
+the direction by clockwise movement of the
+
+107
+00:08:03,960 --> 00:08:09,530
+flagella، تكون حركة عشوائية، ومع عقارب الساعة. هذا
+
+108
+00:08:09,530 --> 00:08:15,050
+بالنسبة لنوع الحركة. هنا
+
+109
+00:08:15,050 --> 00:08:20,370
+في الشرائح، ذكر كل مواقع الأسواط. قلنا لو
+
+110
+00:08:20,370 --> 00:08:24,910
+كانت تحيط بالخلية البكتيرية، أسمي البكتيريا
+
+111
+00:08:24,910 --> 00:08:30,470
+peritrichous. من الأمثلة عليها E. coli. نحفظ المثال
+
+112
+00:08:30,470 --> 00:08:34,690
+E. coli: peritrichous. الفلاجلة تحيط بالخلية
+
+113
+00:08:34,690 --> 00:08:41,230
+البكتيرية. سوط واحد على قطب واحد: monotrichous. مثال
+
+114
+00:08:41,230 --> 00:08:47,530
+عليها Vibrio cholerae. هنا عندي أنا حزمة من
+
+115
+00:08:47,530 --> 00:08:52,850
+الأسواط على قطب واحد، أسميها lophotrichous. ذا
+
+116
+00:08:52,850 --> 00:08:58,030
+كل مثال، Pseudomonas. نحذف هذا، Pseudomonas هي
+
+117
+00:08:58,030 --> 00:09:03,550
+تعتبر monotrichous وليست lophotrichous. يبقى نعدل
+
+118
+00:09:03,550 --> 00:09:06,610
+على الشريحة أن Pseudomonas لا تعتبر lophotrichous،
+
+119
+00:09:06,610 --> 00:09:11,070
+تعتبر monotrichous. يبقى lophotrichous لما
+
+120
+00:09:11,070 --> 00:09:15,270
+يكون في عندي حزمة من الأسواط على قطب واحد. لما يكون
+
+121
+00:09:15,270 --> 00:09:19,470
+في عندي one or more flagella at both poles، لما
+
+122
+00:09:19,470 --> 00:09:22,330
+يكون في عندي سوط واحد أو مجموعة أسواط على كلا
+
+123
+00:09:22,330 --> 00:09:26,410
+القطبين، أسميها amphitrichous. مثال عليها Spirillum
+
+124
+00:09:26,410 --> 00:09:30,230
+وبعض الأنواع الأخرى. يبقى هدول كل الأمثلة التي
+
+125
+00:09:30,230 --> 00:09:34,270
+ذكرناها، تعتبر motile، لكن موقع الأسواط
+
+126
+00:09:34,270 --> 00:09:43,750
+يختلف. motility
+
+127
+00:09:43,750 --> 00:09:49,430
+testing. motility called by، يقول لي: ممكن أنا أكشف
+
+128
+00:09:49,430 --> 00:09:53,310
+عن motility البكتيريا. motility في ثلاث طرق.
+
+129
+00:09:53,310 --> 00:09:58,340
+عشان أعرف هل البكتيريا motile أو non-motile. الطريقة
+
+130
+00:09:58,340 --> 00:10:02,460
+الأولى: Hanging Drop Technique. طريقة ثانية:
+
+131
+00:10:02,460 --> 00:10:06,280
+Flagella stain، الذي يعتبر special stain. الطريقة
+
+132
+00:10:06,280 --> 00:10:10,040
+الثالثة، وهي الأكثر شيوعًا (most common): semisolid media
+
+133
+00:10:10,040 --> 00:10:15,580
+inoculation. أول طريقة، وهي Hanging drop slide.
+
+134
+00:10:15,580 --> 00:10:20,860
+Hanging معناها معلقة. هي تعتبر طريقة مثل الـ wet
+
+135
+00:10:20,860 --> 00:10:24,140
+mount، التي قلنا من خلالها أقدر أعرف
+
+136
+00:10:24,140 --> 00:10:28,450
+bacterial morphology. قلنا في wet mount أقدر أشوف
+
+137
+00:10:28,450 --> 00:10:33,770
+living bacteria، حية، يعني أقدر أشوفها
+
+138
+00:10:33,770 --> 00:10:38,350
+تتحرك، يعني سأرى حركة البكتيريا. لكن لو صبغتها،
+
+139
+00:10:38,350 --> 00:10:42,070
+لن أرى motility، لن أرى البكتيريا تتحرك، لأن
+
+140
+00:10:42,070 --> 00:10:44,670
+أحنا قلنا من خطوات الصباغة: في مرحلة التسخين (heat)، في عملية
+
+141
+00:10:44,670 --> 00:10:50,650
+heat fixation،  توقف الحركة (kills movement). في الـ wet
+
+142
+00:10:50,650 --> 00:10:53,370
+mount العادية التي شرحناها، قلنا إننا نجيب
+
+143
+00:10:53,370 --> 00:10:57,990
+شريحة (slide)، نضع قطرة من المعلق البكتيري (drop of bacterial suspension)، ونضع فوقها
+
+144
+00:10:57,990 --> 00:11:02,030
+غطاء زجاجي (cover slip). هذه الطريقة، wet mount العادية، التي
+
+145
+00:11:02,030 --> 00:11:06,550
+شرحناها،  تُشَكِّل ضغطًا على الشريحة (slide)، فيُعيق
+
+146
+00:11:06,550 --> 00:11:10,090
+حركة البكتيريا. فممكن البكتيريا تكون motile، لكن
+
+147
+00:11:10,090 --> 00:11:16,860
+الضغط الذي يشكله الغطاء الزجاجي (cover slip) على الشريحة (slide) سيعيق حركة
+
+148
+00:11:16,860 --> 00:11:21,840
+البكتيريا. فبالتالي، ممكن أشوفها non-motile. غير أنه
+
+149
+00:11:21,840 --> 00:11:26,460
+في هذه الطريقة لن أقدر أن أميز الحركة الحقيقية (true movement)، حركة
+
+150
+00:11:26,460 --> 00:11:31,780
+البكتيريا بنفسها (by itself)، بواسطة الأسواط، عن حركة البكتيريا،
+
+151
+00:11:31,780 --> 00:11:36,280
+Brownian movement، التي هي حركة جزيئات السائل. يبقى
+
+152
+00:11:36,280 --> 00:11:39,600
+في wet mount العادية، لما أجيب شريحة (slide) وأحط
+
+153
+00:11:39,600 --> 00:11:44,030
+معلقًا بكتيريًا (bacterial suspension) وغطاء زجاجي (cover slip)، سيعيق الحركة
+
+154
+00:11:44,030 --> 00:11:48,810
+البكتيريا لأنها تُشَكِّل ضغطًا. ثاني شيء، لن أقدر أن أميز
+
+155
+00:11:48,810 --> 00:11:52,790
+الحركة الحقيقية (true movement) عن Brownian movement. عشان هيك
+
+156
+00:11:52,790 --> 00:11:56,830
+عملنا تعديلًا (modification) لهذه الطريقة، عشان أقدر
+
+157
+00:11:56,830 --> 00:12:01,350
+أحدد هل البكتيريا motile، تتحرك بنفسها (by itself)، أو non-
+
+158
+00:12:01,350 --> 00:12:06,630
+motile. الطريقة اسمها Hanging drop slide. نستخدم
+
+159
+00:12:06,630 --> 00:12:11,330
+شريحة تُعلِّق فيها البكتيريا، اسمها depression slide.
+
+160
+00:12:12,340 --> 00:12:15,740
+ما هي depression slide؟ أقول: هي شريحة (slide) that has a
+
+161
+00:12:15,740 --> 00:12:19,820
+concavity، يعني لها تقعر، زي ما ملاحظين، هي
+
+162
+00:12:19,820 --> 00:12:25,100
+depression slide. فيها يكون هناك تقعر. هذه
+
+163
+00:12:25,100 --> 00:12:30,860
+الشريحة (slide)  that has a concavity and a cover
+
+164
+00:12:30,860 --> 00:12:34,820
+slip can be placed on it، يعني ممكن أيضًا أني أنا
+
+165
+00:12:34,820 --> 00:12:38,740
+أضع الغطاء الزجاجي (cover slip) فوقها. يبقى هذه هي
+
+166
+00:12:38,740 --> 00:12:42,890
+depression slide. نجيب قطرة من البكتيريا (drop of bacteria)، ونضعها على
+
+167
+00:12:42,890 --> 00:12:46,310
+الغطاء الزجاجي (cover slip)، وبعدها نقلبها، ونضعها فوق depression
+
+168
+00:12:46,310 --> 00:12:51,150
+slide. هكذا، تُعلَّق البكتيريا. طيب، تعلقت طبعًا
+
+169
+00:12:51,150 --> 00:12:55,590
+البكتيريا على السطح، هكذا أقدر بكل سهولة أحدد حركة
+
+170
+00:12:55,590 --> 00:12:59,750
+البكتيريا. طيب، لو كان ما عندي أنا depression slide، و
+
+171
+00:12:59,750 --> 00:13:03,070
+أريد أن أشاهد البكتيريا المتحركة (motile)، طبعًا هي شريحة
+
+172
+00:13:03,070 --> 00:13:06,9
+
+223
+00:16:50,200 --> 00:16:54,370
+لكن بالنسبة لـ Retromovement اللي هي water molecule
+
+224
+00:16:54,370 --> 00:16:59,130
+حركة البكتيريا نفسها runs and tumble اللي هي تعتبر
+
+225
+00:16:59,130 --> 00:17:09,310
+حركة البكتيريا by itself by فلاجلة قلنا
+
+226
+00:17:09,310 --> 00:17:13,510
+عندي ثلاث طرق عشان أعرف هل البكتيريا motile أو non-motile
+
+227
+00:17:13,510 --> 00:17:16,430
+الطريقة الأولى اللي هي hanging drop
+
+228
+00:17:16,430 --> 00:17:20,280
+technique الطريقة الثانية لفلاجلة، استخدم طريقة
+
+229
+00:17:20,280 --> 00:17:23,140
+ثانية عشان أعرف هل البكتيريا non-motile أو  non-
+
+230
+00:17:23,140 --> 00:17:28,220
+motile بدي أصبغ الفلاجلة المسؤول عن الحركة
+
+231
+00:17:28,220 --> 00:17:34,100
+الفلاجلة قلنا هو تنيق ضعيف خفيف لازم أنا أصبغه
+
+232
+00:17:34,100 --> 00:17:37,740
+عشان أقدر أشوفه، flagella should be amplified
+
+233
+00:17:37,740 --> 00:17:41,400
+enlarged لازم أني أنا أكبره، use a standard that is
+
+234
+00:17:41,400 --> 00:17:47,810
+especially deposit on flagella، increasing diameter
+
+235
+00:17:47,810 --> 00:17:52,230
+بقول لي أنا لما استخدم  الليستين، هي الصبغة هتترسب على
+
+236
+00:17:52,230 --> 00:17:55,650
+الـ flagella بالتالي هيزيد قطر الـ flagella، وأقدر
+
+237
+00:17:55,650 --> 00:18:00,730
+أني أنا أشوفه، some
+
+238
+00:18:00,730 --> 00:18:05,170
+في عندي أنا طريقتين للصباغة قبل ما نحكي على
+
+239
+00:18:05,170 --> 00:18:09,250
+الطريقتين، كأي خطوة من خطوات الصباغة، هعمل smearing
+
+240
+00:18:09,250 --> 00:18:13,490
+preparation، air drying لكن الـ fixation هعمل
+
+241
+00:18:13,490 --> 00:18:19,860
+formalin fixation، formalin fixation هعمل، مش هي
+
+242
+00:18:19,860 --> 00:18:25,060
+fixation تمام، رابع خطوة، الـ ستاين اللي قلنا بتختلف
+
+243
+00:18:25,060 --> 00:18:28,180
+حسب نوع الستين، بالنسبة لفلاجلة ستين في عندي
+
+244
+00:18:28,180 --> 00:18:32,020
+طريقتين، الطريقة الأولى بستخدم الـ primary stain
+
+245
+00:18:32,020 --> 00:18:37,360
+rosanilin-dye، يبقى الـ primary stain هي rosanilin-dye
+
+246
+00:18:37,360 --> 00:18:39,000
+بقول لي بستخدمها
+
+247
+00:18:46,070 --> 00:18:49,730
+الـ rose-aniline dye هي الـ primary stain وبستخدم
+
+248
+00:18:49,730 --> 00:18:55,030
+أي ماردانت، تمام، بنفس الطريقة عملت سمير بـ
+
+249
+00:18:55,030 --> 00:18:59,990
+preparation، air drying، formalin fixation، بعدها غمرت
+
+250
+00:18:59,990 --> 00:19:04,370
+الـ slide بالـ rose-aniline dye اللي هي الـ primary
+
+251
+00:19:04,370 --> 00:19:09,290
+stain، عملت washing، حطيت ماردانت، تمام، بعد هيك أنا
+
+252
+00:19:09,290 --> 00:19:13,750
+بقدر أَحدد، اللي هي أشوف تحت الـ microscope اللي هو
+
+253
+00:19:13,750 --> 00:19:18,830
+الـ flagella، هشوفه طبعاً بشكل واضح، وكانتني، لكن أنا
+
+254
+00:19:18,830 --> 00:19:22,190
+بعد ما صبغت، الصبغة هتترسب على الـ Flagella، هقدر
+
+255
+00:19:22,190 --> 00:19:26,690
+أني أنا أشوفه، Apply to bacterial suspension fixed
+
+256
+00:19:26,690 --> 00:19:31,150
+in formalin، طبعاً احنا من حطينا الـ Rosanilin dye and
+
+257
+00:19:31,150 --> 00:19:35,650
+الـ mordant  لبكتيريا الـ suspension اللي أنا إعدتها مسبقاً
+
+258
+00:19:35,650 --> 00:19:39,710
+بالـ Formalin and spread across a glass slide
+
+259
+00:19:39,710 --> 00:19:44,790
+بقول لي الـ Formalin linked to or fixed the flagella
+
+260
+00:19:45,300 --> 00:19:48,260
+إيش وظيفة الـ Formalin اللي هو بيعمل fixation
+
+261
+00:19:48,260 --> 00:19:52,820
+للفلاجلة and other surface proteins of the cell، The
+
+262
+00:19:52,820 --> 00:19:56,120
+dye اللي هي الـ rose aniline dye and mordant then
+
+263
+00:19:56,120 --> 00:20:01,160
+precipitate around this fixed surface، بقول لي الصبغة
+
+264
+00:20:01,160 --> 00:20:05,330
+و الـ mordant هيترسبوا حول اللي هو الـ Flagella
+
+265
+00:20:05,330 --> 00:20:10,750
+اللي تم تثبيته بالـ Formalin، Enlarging their
+
+266
+00:20:10,750 --> 00:20:14,750
+diameter، هيزيد حجم اللي هو الـ Diameter تبع الـ
+
+267
+00:20:14,750 --> 00:20:18,050
+Flagella and making Flagella visible when viewed
+
+268
+00:20:18,050 --> 00:20:23,030
+under the microscope، هيك أنا هقدر إني أشوفه تحت
+
+269
+00:20:23,030 --> 00:20:27,250
+الـ Microscope، بالنسبة للصبغة الروز أنالين داي
+
+270
+00:20:27,250 --> 00:20:31,790
+بتعطي اللون الأحمر، راح يترسب حول الفلاجلة اللي تم
+
+271
+00:20:31,790 --> 00:20:34,870
+تثبيته بالفرمالين، بالتالي هيك هيزيد حجمه، هذه
+
+272
+00:20:34,870 --> 00:20:39,350
+بالنسبة للطريقة الأولى، الطريقة الثانية في الصباغة
+
+273
+00:20:39,350 --> 00:20:43,430
+في الفلاجلة، الستين بقول لي بستخدم الـ silver nitrate
+
+274
+00:20:43,430 --> 00:20:47,290
+كـ primary stain وكموردانت، بستخدم موردانت
+
+275
+00:20:47,290 --> 00:20:51,590
+تلفرقتنات، و الـ silver nitrate، يبقى بعمل smear
+
+276
+00:20:51,590 --> 00:20:56,570
+preparation، air drying، formalin fixation، الصبغة
+
+277
+00:20:56,570 --> 00:21:01,010
+سيلفر نيترات هتعطي لون أسود، والموردانت في
+
+278
+00:21:01,010 --> 00:21:05,130
+الـ تلفرقتنات، موردانت، بقول لي هذه الصبغة مع الموردانت
+
+279
+00:21:05,130 --> 00:21:09,090
+هيتم تعريضها للبكتيريا الـ suspension، النتيجة
+
+280
+00:21:09,090 --> 00:21:12,710
+هيتكون dark precipitates that form on the bacteria
+
+281
+00:21:12,710 --> 00:21:16,010
+and their and their flagella، allow them to be
+
+282
+00:21:16,010 --> 00:21:20,020
+easily، هقدر إني أنا أشوفها تحت الماكروسكوب بكل
+
+283
+00:21:20,020 --> 00:21:23,400
+سهولة، الصبغة والموردانت هيترسبوا على الفلاجلة
+
+284
+00:21:23,400 --> 00:21:27,160
+فبالتالي هيزيد الـ diameter للفلاجلة، هقدر إني أنا
+
+285
+00:21:27,160 --> 00:21:31,220
+أشوفها، Silver Nitrate قلنا بيعطي اللون الأسود
+
+286
+00:21:31,220 --> 00:21:35,460
+Silver plating Technique، هاي الطريقة بستخدمها
+
+287
+00:21:35,460 --> 00:21:39,860
+used to stain the very slender spirochete، بقول لي
+
+288
+00:21:39,860 --> 00:21:43,260
+هاي الطريقة أنا بستخدمها في بكتيريا لصباغة هذه
+
+289
+00:21:43,260 --> 00:21:48,880
+البكتيريا very slender spirochete، بكتيريا ماخدة
+
+290
+00:21:48,880 --> 00:21:52,860
+شكل الـ Spiral، هاي الطريقة اللي هي الـ Silver
+
+291
+00:21:52,860 --> 00:21:57,360
+Nitrate والتلفرقتنات Mordant، يبقى في هاي الطريقة
+
+292
+00:21:57,360 --> 00:22:00,840
+بستخدم الـ Silver Nitrate كـ Primary Stain و
+
+293
+00:22:00,840 --> 00:22:04,880
+Mordant، التلفرقتنات هضيفهم للـ Bacterial Suspension
+
+294
+00:22:04,880 --> 00:22:10,440
+هيترسبوا حول الفلاجلة، وبالتالي هيعطي اللون الأسود
+
+295
+00:22:10,440 --> 00:22:14,770
+بالتالي سهل إني أنا أشوفهم تحت الـ microscope، هذه
+
+296
+00:22:14,770 --> 00:22:19,390
+الطريقة الثانية، ملاحظين في الصورة الفلاجلة أقدرت
+
+297
+00:22:19,390 --> 00:22:23,650
+إني أنا أشوفه، هي مسبوخة بالروزانالين داي اللي بتعطي
+
+298
+00:22:23,650 --> 00:22:28,690
+اللون الزهري، لكن بالنسبة لهذه الصورة، المسبوخة بالـ
+
+299
+00:22:28,690 --> 00:22:33,070
+silver nitrate زائد التلفرقتنات هيعطي اللون
+
+300
+00:22:33,070 --> 00:22:38,230
+الأسود، آخر طريقة في اللي هو الـ bacterial motility
+
+301
+00:22:38,230 --> 00:22:42,630
+اللي هي semi-solid media inoculation، اللي هي الـ
+
+302
+00:22:42,630 --> 00:22:46,130
+most common، the most commonly used test for
+
+303
+00:22:46,130 --> 00:22:49,770
+motility in microbiology، لأ هي أكثرها شيوعاً
+
+304
+00:22:49,770 --> 00:22:53,890
+واستخداماً في معمل الـ microbiology عشان أَحدد هل
+
+305
+00:22:53,890 --> 00:22:58,410
+البكتيريا motile or non-motile، بقول لي it depends
+
+306
+00:22:58,410 --> 00:23:01,850
+on the ability of motile bacteria to move through
+
+307
+00:23:01,850 --> 00:23:06,570
+semi-solid media، هي بتعتمد على قدرة البكتيريا على
+
+308
+00:23:06,570 --> 00:23:11,770
+الحركة في semi-solid media، الميديا قلنا هي وسط غذائي
+
+309
+00:23:11,770 --> 00:23:16,950
+لنمو البكتيريا، بقسم الميديا حسب نسبة الأجار طبعاً
+
+310
+00:23:16,950 --> 00:23:20,650
+الأجار اللي يعتبر هو solidifying agent اللي هو
+
+311
+00:23:20,650 --> 00:23:27,930
+بيَخْلِي الوسط صلب، ممكن الميديا تكون solid وممكن تكون
+
+312
+00:23:27,930 --> 00:23:32,680
+semi-solid وممكن تكون liquid، لما تكون solid بتكون
+
+313
+00:23:32,680 --> 00:23:37,920
+نسبة الأجار من 1.5 لـ 2% أجار، لو كانت semi-solid
+
+314
+00:23:37,920 --> 00:23:41,460
+اللي أنا بستخدمها في فحص الـ Motility، نسبة الأجار
+
+315
+00:23:41,460 --> 00:23:48,380
+من 0.4 لـ 0.1% أجار، لكن بالنسبة لو كانت liquid أكيد
+
+316
+00:23:48,380 --> 00:23:53,920
+هتكون no أجار، لأنه ما صارشي، هي broth ضلت سائلة ما
+
+317
+00:23:53,920 --> 00:23:58,180
+اتصلبت، يبقى توزيع أو تقسيم الـ media على حسب اللي
+
+318
+00:23:58,180 --> 00:24:03,490
+هو نسبة الأجار، solid تكون نسبة الأجار من 1.5% لـ 2%
+
+319
+00:24:03,490 --> 00:24:09,550
+semi-solid 0.4 من 0.1%، Liquid مافيش أي نسبة أجار، Zero
+
+320
+00:24:09,550 --> 00:24:16,710
+كيف أنا بدي أعمل الفحص، قبل ما نحكي على كيف أنا الـ
+
+321
+00:24:16,710 --> 00:24:19,890
+procedure لهذا الفحص، فحص الـ modality لازم يكون
+
+322
+00:24:19,890 --> 00:24:24,970
+عندي أنا semi-solid، يعني نسبة الأجار 0.4 من 0.1%
+
+323
+00:24:24,970 --> 00:24:29,520
+لأنه أنا لو استخدمت solid media أكيد البكتيريا مش
+
+324
+00:24:29,520 --> 00:24:34,240
+هتتحرك، هيكون صلب، لو استخدمت liquid هتتحرك وتنتشر
+
+325
+00:24:34,240 --> 00:24:38,460
+في كل tube ومش هعرف هل هي true movement أو
+
+326
+00:24:38,460 --> 00:24:42,520
+Brownian movement اللي هي حركة جزيئات السائل، فلازم
+
+327
+00:24:42,520 --> 00:24:47,440
+أنا استخدم semi-solid media، طبعاً أنا هاي الـ Semi
+
+328
+00:24:47,440 --> 00:24:51,780
+solid media بصوبها في tube، تمام، احنا قلنا نحضر الـ
+
+329
+00:24:51,780 --> 00:24:56,660
+media بصوبها، ممكن يكون في قوارير لكن في الـ semi
+
+330
+00:24:56,660 --> 00:25:02,480
+solid media بصوبها في tube، بتركها تتصلب، فتعطيني
+
+331
+00:25:02,480 --> 00:25:07,760
+اللي هي step، هنتعرف عليها بالتفصيل في المعمل الجاي
+
+332
+00:25:07,760 --> 00:25:09,840
+هيك بتكون شكل التيوب
+
+333
+00:25:16,030 --> 00:25:21,030
+التيوب، بصب الـ media فيه، هلاقيه زي الجل، متصلب، هاي
+
+334
+00:25:21,030 --> 00:25:26,250
+الـ .. هذا الشيء بسميه stabbing لأني هعمل طعن في الـ
+
+335
+00:25:26,250 --> 00:25:31,670
+tube، هنتعرف هلأ بالتفصيل، كيف خطوات الشغل، خطوات
+
+336
+00:25:31,670 --> 00:25:36,830
+الشغل، بقول لي أنا بستخدم sterile needle، إيش يعني
+
+337
+00:25:36,830 --> 00:25:41,450
+sterile needle، بالنسبة للـ inoculating loop، الـ inoculating
+
+338
+00:25:41,450 --> 00:25:44,750
+loop، بقول لي احنا قلنا في عندي loop، الـ loop ممكن
+
+339
+00:25:44,750 --> 00:25:49,790
+تكون inoculating loop، الـ inoculating loop high فيه
+
+340
+00:25:49,790 --> 00:25:58,290
+بكون إلها حلقة، لكن الـ inoculating needle، الـ loop ما
+
+341
+00:25:58,290 --> 00:26:03,890
+إلها حلقة، يبقى الـ loop high بسميها inoculating
+
+342
+00:26:03,890 --> 00:26:07,310
+loop، فيه موجود فيها حلقة، high ما بستخدمها في فحص
+
+343
+00:26:07,310 --> 00:26:12,170
+الـ motility، لكن الـ inoculating needle هي الـ needle
+
+344
+00:26:12,170 --> 00:26:16,190
+بستخدمها في فحص الـ motility، باخد اللي هو الـ colony
+
+345
+00:26:16,190 --> 00:26:22,670
+من الطبق وبعدين هزرعها في، هاي، يوم طيب، الخطوة
+
+346
+00:26:22,670 --> 00:26:25,830
+الثانية، يبقى عرفنا إيش يعني الـ needle، اللي هي loop
+
+347
+00:26:25,830 --> 00:26:29,510
+لكن ما إلها حلقة، بستخدمها في فحص اللي هو الـ
+
+348
+00:26:29,510 --> 00:26:33,980
+motility أو أي فحص فيه اللي هو stabbing، باخد أي
+
+349
+00:26:33,980 --> 00:26:37,740
+colony من الـ organism اللي أنا ببحث هل هو motile أو
+
+350
+00:26:37,740 --> 00:26:41,900
+non-motile، الخطوة الثانية، Stab quickly a tube of
+
+351
+00:26:41,900 --> 00:26:45,860
+semi-solid media، بقول لي اعملي Stabbing، إيش يعني
+
+352
+00:26:45,860 --> 00:26:48,580
+Stabbing يعني اعملي طعنة في الـ tube اللي أنتِ
+
+353
+00:26:48,580 --> 00:26:51,800
+جهزتيها، semi-solid media، الميديا اللي بتحتوي على
+
+354
+00:26:51,800 --> 00:26:56,720
+0.4 من 0.1% أجار، كيف بدي أعمل؟ بقول لنا
+
+355
+00:26:56,720 --> 00:27:00,440
+بتجيبي الـ inoculating needle بعد ما أخذتي من
+
+356
+00:27:00,440 --> 00:27:05,080
+البكتيريا من الطبق اللي بدك تشوفي هل هو البكتيريا
+
+357
+00:27:05,080 --> 00:27:08,440
+motile أو non-motile، بتدخلي الـ inoculating needle
+
+358
+00:27:08,440 --> 00:27:14,220
+وبتعمل طعنة تمام، بسرعة بتعملي طعنة في التيوب
+
+359
+00:27:14,220 --> 00:27:18,560
+طبعاً مش للآخر، تقريباً بتوصلي إلى الثلثين وبتعملي
+
+360
+00:27:18,560 --> 00:27:23,690
+طعنة وبتطلعي بسرعة، بعد ما تخلصي بتعملي incubation
+
+361
+00:27:23,690 --> 00:27:28,050
+the semi-solid media لمدة 24 ساعة في الـ incubator
+
+362
+00:27:28,050 --> 00:27:31,690
+بدي أعمل incubation، بحطه في الـ incubator لمدة 24
+
+363
+00:27:31,690 --> 00:27:38,290
+ساعة، بعد 24 ساعة بقرأ النتيجة، قبل ما أنا أعمل
+
+364
+00:27:38,290 --> 00:27:43,450
+stabbing، لما أَفتِح الـ tube طبعاً لازم احنا من الـ
+
+365
+00:27:43,450 --> 00:27:48,030
+aseptic technique، أعقم فوهة الـ tube، وبعد ما أخلص
+
+366
+00:27:48,030 --> 00:27:52,690
+نفس الشيء، أعقم فوهة الـ tube، هذا من الـ Aseptic
+
+367
+00:27:52,690 --> 00:27:58,430
+technique، بالنسبة للنتيجة، النتيجة بتكون بعد 24
+
+368
+00:27:58,430 --> 00:28:02,530
+ساعة، كيف بدي أفسر النتيجة؟ بقول لي pattern of growth
+
+369
+00:28:02,530 --> 00:28:06,550
+of motile organism، the intermediate is turbid with
+
+370
+00:28:06,550 --> 00:28:09,730
+the growth of organism which has moved away from
+
+371
+00:28:09,730 --> 00:28:13,870
+the stab line، بقول لي هان لو كانت البكتيريا motile
+
+372
+00:28:13,870 --> 00:28:19,330
+كيف بدي أعرف؟ بقول لي أن نمو البكتيريا بيكون، بتتحرك
+
+373
+00:28:19,330 --> 00:28:23,870
+على خط الزراعة وكمان بلاحظ إن في أنها تحركت كمان
+
+374
+00:28:23,870 --> 00:28:28,170
+بعيداً عن خط الزراعة، يبقى لو كانت motile الـ media
+
+375
+00:28:28,170 --> 00:28:31,270
+هتكون أكيد turbid لأنها هتنمو، البكتيريا سواء كانت
+
+376
+00:28:31,270 --> 00:28:35,150
+motile أو non-motile، هيكون في عندي turbidity لكن
+
+377
+00:28:35,150 --> 00:28:40,070
+كيف بدي أَحدد هل هي motile أو non-motile؟ بقول لي أنّه
+
+378
+00:28:40,070 --> 00:28:44,350
+في الـ motile بتتحرك بعيداً عن خط الزراعة لكن في الـ
+
+379
+00:28:44,350 --> 00:28:48,300
+non-motile فقط بلاقي الـ turbidity على خط الزراعة
+
+380
+00:28:48,300 --> 00:28:52,200
+لأنه ما قدرت تتحرك بعيداً، فقط دلت على خط الزراعة
+
+381
+00:28:52,200 --> 00:28:57,300
+لأنه نمت البكتيريا، pattern هيكون has moved away from
+
+382
+00:28:57,300 --> 00:29:00,820
+the stab line، الـ motile بتتحرك بعيداً عن خط الزراعة
+
+383
+00:29:00,820 --> 00:29:03,920
+الـ stab line يعني خط الزراعة، pattern of growth of
+
+384
+00:29:03,920 --> 00:29:08,860
+a non-motile organism، only the stab line is turbid
+
+385
+00:29:08,860 --> 00:29:12,640
+with growth، هلاقي فقط أنّه مو الـ turbidity فقط على
+
+386
+00:29:12,640 --> 00:29:17,130
+خط الزراعة، بعض الأحيان ممكن أنا ما أشوف هاي الـ
+
+387
+00:29:17,130 --> 00:29:21,910
+turbidity، فممكن أضيف color indicator، indicator  كي
+
+388
+00:29:21,910 --> 00:29:24,730
+أشوف على الـ media، أضيفه قبل وأنا بحضر الـ media
+
+389
+00:29:24,730 --> 00:29:29,250
+ممكن أضيف indicator، هذا الـ indicator هيتلون الـ
+
+390
+00:29:29,250 --> 00:29:33,130
+turbidity تبع البكتيريا بلون الـ indicator، وهيك أنا
+
+391
+00:29:33,130 --> 00:29:35,870
+بقدر أشوف الـ turbidity بشكل واضح
+
+392
+00:29:38,670 --> 00:29:41,930
+يبقى الـ Motile والـ Non-motile هيكون فيه turbidity
+
+393
+00:29:41,930 --> 00:29:46,610
+لكن الفرق أن الـ Motility هتلاقي الترbidity بعيداً عن
+
+394
+00:29:46,610 --> 00
\ No newline at end of file
diff --git a/PL9fwy3NUQKwZWgAb_V1ATlRRsH-6i3cPC/9AIy0KlGCSw_raw.json b/PL9fwy3NUQKwZWgAb_V1ATlRRsH-6i3cPC/9AIy0KlGCSw_raw.json
new file mode 100644
index 0000000000000000000000000000000000000000..6fb0e220c6aa7765dc83cd74df77fec65fda29df
--- /dev/null
+++ b/PL9fwy3NUQKwZWgAb_V1ATlRRsH-6i3cPC/9AIy0KlGCSw_raw.json
@@ -0,0 +1 @@
+{"segments": [{"id": 1, "seek": 2716, "start": 0.72, "end": 27.16, "text": "السلام عليكم ورحمة الله وبركاته يعطيكم العافية جميعا في هذا اللقاء هنتكلم عن ال bacterial motility حركة البكتيريا البكتيريا بتتحرك بواسطة الفلاجلة وعرفنا أن الفلاجلة يعتبر special component يعني في بكتيريا عندي motile وفي بكتيريا non motile عشان أعرف هل البكتيريا متحركة أو لأ في عندي تلت طرق هنتعرف عليها في هذه المحاضرة", "tokens": [6027, 3794, 37440, 25894, 24793, 4032, 2288, 35571, 3660, 21984, 4032, 26890, 4117, 9307, 3224, 37495, 9566, 1829, 24793, 18863, 31845, 10632, 10874, 2304, 40228, 995, 8978, 23758, 13672, 4587, 16606, 8032, 29399, 4117, 19528, 18871, 2423, 35632, 2184, 1140, 11331, 31747, 3660, 29739, 4117, 2655, 13546, 25528, 29739, 4117, 2655, 13546, 25528, 39894, 2655, 5016, 31747, 4724, 14407, 3794, 9566, 3660, 27188, 1211, 26108, 37977, 4032, 3615, 28480, 8315, 14739, 27188, 1211, 26108, 37977, 7251, 34268, 26890, 2121, 6542, 37495, 22653, 8978, 4724, 4117, 2655, 13546, 25528, 18871, 16254, 2184, 794, 4032, 41185, 4724, 4117, 2655, 13546, 25528, 2107, 2184, 794, 6225, 8592, 7649, 5551, 3615, 28480, 8032, 1211, 29739, 4117, 2655, 13546, 25528, 44650, 5016, 31747, 3660, 34051, 5296, 10721, 8978, 18871, 16254, 6055, 1211, 2655, 23032, 2288, 4587, 8032, 29399, 3615, 28480, 25894, 11296, 8978, 29538, 9673, 5016, 46958, 25720], "avg_logprob": -0.09082031524222758, "compression_ratio": 1.9781021897810218, "no_speech_prob": 1.6033649444580078e-05, "words": [{"start": 0.72, "end": 1.06, "word": "السلام", "probability": 0.8961588541666666}, {"start": 1.06, "end": 1.52, "word": " عليكم", "probability": 0.952392578125}, {"start": 1.52, "end": 1.98, "word": " ورحمة", "probability": 0.9442138671875}, {"start": 1.98, "end": 2.3, "word": " الله", "probability": 0.9765625}, {"start": 2.3, "end": 3.12, "word": " وبركاته", "probability": 0.98134765625}, {"start": 3.12, "end": 3.76, "word": " يعطيكم", "probability": 0.8743896484375}, {"start": 3.76, "end": 4.22, "word": " العافية", "probability": 0.97998046875}, {"start": 4.22, "end": 4.8, "word": " جميعا", "probability": 0.910400390625}, {"start": 4.8, "end": 5.16, "word": " في", "probability": 0.61279296875}, {"start": 5.16, "end": 5.36, "word": " هذا", "probability": 0.8525390625}, {"start": 5.36, "end": 5.84, "word": " اللقاء", "probability": 0.9900716145833334}, {"start": 5.84, "end": 6.54, "word": " هنتكلم", "probability": 0.9727783203125}, {"start": 6.54, "end": 6.92, "word": " عن", "probability": 0.99462890625}, {"start": 6.92, "end": 7.16, "word": " ال", "probability": 0.92236328125}, {"start": 7.16, "end": 7.78, "word": " bacterial", "probability": 0.34228515625}, {"start": 7.78, "end": 8.56, "word": " motility", "probability": 0.964111328125}, {"start": 8.56, "end": 9.46, "word": " حركة", "probability": 0.955078125}, {"start": 9.46, "end": 10.24, "word": " البكتيريا", "probability": 0.921484375}, {"start": 10.24, "end": 11.12, "word": " البكتيريا", "probability": 0.98134765625}, {"start": 11.12, "end": 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cell wall بيربط و بيدعم ال filament و ال hook بال cell wall فى عندي رسمة هى ال filament اللى قولنا بتكون من بروتين لفلاجلين فى عندي ال hook الخطافة هو قاعدة ال filament و فى عندي البازل باضى اللى بيربط ال hook و ال filament بال cell wall", "tokens": [3555, 31377, 1211, 4724, 46958, 7578, 19753, 34740, 3714, 30544, 29739, 31377, 1211, 4724, 46958, 7578, 4724, 1829, 39648, 32239, 4724, 13546, 3555, 9566, 16472, 4117, 9640, 4724, 13546, 3555, 9566, 1975, 2407, 4724, 25708, 25957, 2423, 44280, 4032, 2423, 6328, 281, 264, 2815, 2929, 4724, 13546, 3555, 9566, 4032, 4724, 25708, 25957, 2423, 44280, 4032, 2423, 6328, 20666, 2815, 2929, 6156, 7578, 18871, 16254, 12602, 38251, 3660, 8032, 7578, 2423, 44280, 13672, 7578, 12174, 12610, 8315, 39894, 30544, 9154, 4724, 32887, 2655, 9957, 5296, 5172, 1211, 26108, 1211, 9957, 6156, 7578, 18871, 16254, 2423, 6328, 33962, 9566, 31845, 3660, 31439, 12174, 995, 22488, 3660, 2423, 44280, 4032, 6156, 7578, 18871, 16254, 29739, 31377, 1211, 4724, 46958, 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بتحيط بالخلية البكتيرية بسمي البكتيريا periatriace من الأمثلة عليها ال E.coli نحفظ المثال E.coli periatriace الفلاجلة بتحيط بالخلية البكتيرية", "tokens": [3224, 15730, 20666, 1863, 35457, 3660, 24976, 39184, 2010, 295, 3963, 34105, 8978, 21136, 15040, 1829, 3215, 9307, 29910, 995, 28242, 2423, 3621, 295, 932, 609, 3505, 12174, 1211, 8315, 45164, 25961, 2655, 39894, 5016, 1829, 9566, 20666, 9778, 1211, 10632, 29739, 4117, 2655, 13546, 10632, 4724, 38251, 1829, 29739, 4117, 2655, 13546, 25528, 680, 7676, 470, 617, 9154, 16247, 2304, 12984, 37977, 25894, 11296, 2423, 462, 13, 8768, 72, 8717, 5016, 5172, 19913, 9673, 12984, 6027, 462, 13, 8768, 72, 680, 7676, 470, 617, 27188, 1211, 26108, 37977, 39894, 5016, 1829, 9566, 20666, 9778, 1211, 10632, 29739, 4117, 2655, 13546, 10632], "avg_logprob": -0.22679005577726272, "compression_ratio": 1.7572815533980584, "no_speech_prob": 2.1457672119140625e-06, "words": [{"start": 489.15, "end": 489.53, "word": "هذا", "probability": 0.939697265625}, 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498.87, "word": " of", "probability": 0.966796875}, {"start": 498.87, "end": 499.63, "word": " flagella", "probability": 0.786865234375}, {"start": 499.63, "end": 500.19, "word": " قلنا", "probability": 0.7763671875}, {"start": 500.19, "end": 500.37, "word": " لو", "probability": 0.9267578125}, {"start": 500.37, "end": 500.79, "word": " كانت", "probability": 0.9892578125}, {"start": 500.79, "end": 501.51, "word": " بتحيط", "probability": 0.9466552734375}, {"start": 501.51, "end": 502.43, "word": " بالخلية", "probability": 0.9024658203125}, {"start": 502.43, "end": 503.29, "word": " البكتيرية", "probability": 0.94736328125}, {"start": 503.29, "end": 504.05, "word": " بسمي", "probability": 0.9241536458333334}, {"start": 504.05, "end": 504.91, "word": " البكتيريا", "probability": 0.88984375}, {"start": 504.91, "end": 506.21, "word": " periatriace", "probability": 0.35089111328125}, {"start": 506.21, "end": 506.77, "word": " من", "probability": 0.52880859375}, {"start": 506.77, "end": 507.45, "word": " الأمثلة", "probability": 0.9793701171875}, {"start": 507.45, "end": 507.91, "word": " عليها", "probability": 0.94970703125}, {"start": 507.91, "end": 508.09, "word": " ال", "probability": 0.8974609375}, {"start": 508.09, "end": 508.37, "word": " E", "probability": 0.284912109375}, {"start": 508.37, "end": 508.85, "word": ".coli", "probability": 0.7516276041666666}, {"start": 508.85, "end": 509.67, "word": " نحفظ", "probability": 0.9803466796875}, {"start": 509.67, "end": 510.47, "word": " المثال", "probability": 0.9817708333333334}, {"start": 510.47, "end": 511.19, "word": " E", "probability": 0.857421875}, {"start": 511.19, "end": 511.71, "word": ".coli", "probability": 0.9085286458333334}, {"start": 511.71, "end": 512.81, "word": " periatriace", "probability": 0.787109375}, {"start": 512.81, "end": 513.53, "word": " الفلاجلة", "probability": 0.764892578125}, {"start": 513.53, "end": 514.07, "word": " بتحيط", "probability": 0.984619140625}, {"start": 514.07, "end": 514.69, "word": " بالخلية", "probability": 0.97412109375}, {"start": 514.69, "end": 515.63, "word": " البكتيرية", "probability": 0.98857421875}], "temperature": 1.0}, {"id": 20, "seek": 54179, "start": 516.85, "end": 541.79, "text": "صوت واحد على قطب واحد mono-tracheas مثال عليها الـ Vibrio cholera هنا عندي أنا حزمة من الأصوات على صوت واحد بسميها الـ Lo-Fo Tracheas ذا كل مثال الـ Sodomonas نحذف هذا ال Sodomonas هي تعتبر mono-tracheas وليس Lo-Fo Tracheas", "tokens": [9381, 35473, 36764, 24401, 15844, 12174, 9566, 3555, 36764, 24401, 35624, 12, 6903, 6000, 296, 50113, 6027, 25894, 11296, 2423, 39184, 691, 897, 6584, 20961, 1663, 34105, 18871, 16254, 41850, 11331, 11622, 46007, 9154, 16247, 9381, 2407, 9307, 15844, 20328, 35473, 36764, 24401, 4724, 38251, 1829, 11296, 2423, 39184, 6130, 12, 37, 78, 1765, 6000, 296, 29910, 995, 28242, 50113, 6027, 2423, 39184, 42059, 24488, 296, 8717, 5016, 8848, 5172, 23758, 2423, 42059, 24488, 296, 39896, 6055, 34268, 26890, 35624, 12, 6903, 6000, 296, 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Sodomonas", "probability": 0.8606770833333334}, {"start": 537.53, "end": 538.03, "word": " هي", "probability": 0.830078125}, {"start": 538.03, "end": 538.85, "word": " تعتبر", "probability": 0.9348958333333334}, {"start": 538.85, "end": 539.33, "word": " mono", "probability": 0.5693359375}, {"start": 539.33, "end": 540.01, "word": "-tracheas", "probability": 0.9656982421875}, {"start": 540.01, "end": 540.49, "word": " وليس", "probability": 0.8513997395833334}, {"start": 540.49, "end": 540.71, "word": " Lo", "probability": 0.8681640625}, {"start": 540.71, "end": 541.05, "word": "-Fo", "probability": 0.9705403645833334}, {"start": 541.05, "end": 541.79, "word": " Tracheas", "probability": 0.94970703125}], "temperature": 1.0}, {"id": 21, "seek": 56801, "start": 542.43, "end": 568.01, "text": "يبقى نعدل على ال slide أن الـ Sodomonas لا تعتبر Loft Tracheas تعتبر Monotracheas يبقى Loft Tracheas لما يكون في عندي حزمة من الأصوات على قطب واحد لما يكون في عندي one or more flagella at both poles لما يكون في عندي صوت واحد أو مجموعة أصوات على كلا القطبين بسميها Amphitracheas مثال عليها الـ Spirulum والتانس", "tokens": [1829, 3555, 4587, 7578, 8717, 22488, 1211, 15844, 2423, 4137, 14739, 2423, 39184, 42059, 24488, 296, 20193, 6055, 34268, 26890, 6130, 844, 1765, 6000, 296, 6055, 34268, 26890, 4713, 310, 81, 6000, 296, 7251, 3555, 4587, 7578, 6130, 844, 1765, 6000, 296, 5296, 15042, 7251, 30544, 8978, 18871, 16254, 11331, 11622, 46007, 9154, 16247, 9381, 2407, 9307, 15844, 12174, 9566, 3555, 36764, 24401, 5296, 15042, 7251, 30544, 8978, 18871, 16254, 472, 420, 544, 932, 609, 3505, 412, 1293, 24760, 5296, 15042, 7251, 30544, 8978, 18871, 16254, 20328, 35473, 36764, 24401, 34051, 3714, 7435, 2304, 2407, 27884, 5551, 9381, 2407, 9307, 15844, 9122, 15040, 25062, 9566, 3555, 9957, 4724, 38251, 1829, 11296, 2012, 950, 270, 81, 6000, 296, 50113, 6027, 25894, 11296, 2423, 39184, 1738, 347, 425, 449, 16070, 2655, 7649, 3794], "avg_logprob": -0.18655303639895987, "compression_ratio": 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561.91, "end": 562.33, "word": " كلا", "probability": 0.71923828125}, {"start": 562.33, "end": 563.05, "word": " القطبين", "probability": 0.908935546875}, {"start": 563.05, "end": 563.67, "word": " بسميها", "probability": 0.9759521484375}, {"start": 563.67, "end": 565.13, "word": " Amphitracheas", "probability": 0.8295084635416666}, {"start": 565.13, "end": 565.85, "word": " مثال", "probability": 0.82421875}, {"start": 565.85, "end": 566.23, "word": " عليها", "probability": 0.959716796875}, {"start": 566.23, "end": 566.41, "word": " الـ", "probability": 0.78125}, {"start": 566.41, "end": 567.11, "word": " Spirulum", "probability": 0.6337890625}, {"start": 567.11, "end": 568.01, "word": " والتانس", "probability": 0.74688720703125}], "temperature": 1.0}, {"id": 22, "seek": 59725, "start": 568.47, "end": 597.25, "text": "تمام يبقى هدول كل الأمثلة اللي ذكرناها يعتبروا motile لكن ال site of flagella بيختلف motility testing motility called by بيقوللي ممكن أنا أكشف عن ال motility عن البكتيريا ال motility في تلت طرق عشان أعرف هل البكتيريا motile or non motile", "tokens": [39237, 10943, 7251, 3555, 4587, 7578, 8032, 3215, 12610, 28242, 16247, 2304, 12984, 37977, 13672, 1829, 29910, 37983, 8315, 11296, 7251, 34268, 26890, 14407, 2184, 794, 44381, 2423, 3621, 295, 932, 609, 3505, 4724, 1829, 46456, 46538, 2184, 1140, 4997, 2184, 1140, 1219, 538, 4724, 1829, 39648, 20292, 3714, 43020, 41850, 5551, 4117, 8592, 5172, 18871, 2423, 2184, 1140, 18871, 29739, 4117, 2655, 13546, 25528, 2423, 2184, 1140, 8978, 6055, 1211, 2655, 23032, 2288, 4587, 6225, 8592, 7649, 5551, 3615, 28480, 8032, 1211, 29739, 4117, 2655, 13546, 25528, 2184, 794, 420, 2107, 2184, 794], "avg_logprob": -0.18700658522154157, "compression_ratio": 1.6778846153846154, "no_speech_prob": 9.28044319152832e-05, "words": [{"start": 568.47, "end": 568.93, "word": "تمام", "probability": 0.670166015625}, {"start": 568.93, "end": 569.15, "word": " يبقى", "probability": 0.9439697265625}, {"start": 569.15, "end": 569.41, "word": 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فيه عشان نسكر ال tube هذا ال parafilm بروح انا بلف ال parafilm بجدل ال parafilm و بعمل دائرة زي دائرة", "tokens": [9566, 3555, 1975, 8315, 19446, 3615, 1863, 16254, 8032, 47302, 2423, 4137, 3714, 43020, 1975, 28543, 2288, 1975, 24401, 3215, 2423, 35632, 2184, 1140, 1975, 28543, 2288, 1975, 25957, 1211, 1975, 8592, 1829, 1975, 25957, 1211, 30767, 1829, 2423, 10799, 4137, 4724, 39648, 8315, 1975, 3224, 39894, 7435, 1829, 3555, 4137, 6225, 18513, 10632, 46811, 10943, 39894, 7435, 1829, 3555, 4137, 6225, 18513, 10632, 4032, 4724, 7435, 1829, 3555, 2423, 1690, 37023, 2423, 1690, 37023, 31439, 4032, 2288, 4587, 4724, 2304, 17082, 9566, 3555, 8717, 14851, 9778, 40448, 3224, 5551, 5016, 1829, 7649, 995, 8978, 3224, 6225, 8592, 7649, 8717, 3794, 37983, 2423, 9917, 23758, 2423, 1690, 37023, 4724, 32887, 5016, 1975, 8315, 4724, 46538, 2423, 1690, 37023, 4724, 7435, 3215, 1211, 2423, 1690, 37023, 4032, 4724, 25957, 1211, 11778, 16373, 25720, 30767, 1829, 11778, 16373, 25720], "avg_logprob": 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" ال", "probability": 0.87451171875}, {"start": 1567.47, "end": 1567.93, "word": " motility", "probability": 0.868896484375}, {"start": 1567.93, "end": 1568.95, "word": " لكن", "probability": 0.95263671875}, {"start": 1568.95, "end": 1570.21, "word": " الانيكولاتينج", "probability": 0.95001220703125}, {"start": 1570.21, "end": 1570.69, "word": " needle", "probability": 0.98388671875}], "temperature": 1.0}, {"id": 59, "seek": 159253, "start": 1571.09, "end": 1592.53, "text": "هي ال needle بستخدمها في فحص ال motility باخد اللي هو ال colony من الطبق وبعدين هزرعها في هايلة يوم طيب الخطوة التانية يبقى عرفنا إيش يعني ال needle اللي هي loop لكن مايلها حلقة بستخدمها في فحص اللي هو ال motility أو أي فحص فيه اللي هو stabbing", "tokens": [3224, 1829, 2423, 11037, 4724, 14851, 9778, 40448, 11296, 8978, 6156, 5016, 9381, 2423, 2184, 1140, 4724, 47283, 3215, 13672, 1829, 31439, 2423, 23028, 9154, 41950, 3555, 4587, 46599, 22488, 9957, 8032, 11622, 2288, 3615, 11296, 8978, 8032, 995, 26895, 3660, 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"word": " هو", "probability": 0.94677734375}, {"start": 1848.47, "end": 1848.61, "word": " ال", "probability": 0.84130859375}, {"start": 1848.61, "end": 1848.89, "word": " color", "probability": 0.83447265625}, {"start": 1848.89, "end": 1849.47, "word": " indicator", "probability": 0.97607421875}, {"start": 1849.47, "end": 1849.71, "word": " احنا", "probability": 0.9205729166666666}, {"start": 1849.71, "end": 1849.97, "word": " قلنا", "probability": 0.8362630208333334}, {"start": 1849.97, "end": 1850.21, "word": " ممكن", "probability": 0.84716796875}, {"start": 1850.21, "end": 1850.49, "word": " عشان", "probability": 0.984375}, {"start": 1850.49, "end": 1850.87, "word": " اقدر", "probability": 0.8605143229166666}, {"start": 1850.87, "end": 1850.99, "word": " ان", "probability": 0.7080078125}, {"start": 1850.99, "end": 1851.23, "word": " انا", "probability": 0.796630859375}, {"start": 1851.23, "end": 1851.63, "word": " اشوف", "probability": 0.9134114583333334}, {"start": 1851.63, "end": 1852.11, "word": " بشكل", "probability": 0.9720052083333334}, {"start": 1852.11, "end": 1852.55, "word": " واضح", "probability": 0.9781901041666666}, {"start": 1852.55, "end": 1852.83, "word": " ممكن", "probability": 0.980224609375}, {"start": 1852.83, "end": 1853.43, "word": " استخدم", "probability": 0.9453125}, {"start": 1853.43, "end": 1854.25, "word": " indicator", "probability": 0.93701171875}], "temperature": 1.0}, {"id": 70, "seek": 188066, "start": 1854.86, "end": 1880.66, "text": "تمام اضيفه على ال media عشان اشوف انمو العكارة بشكل واضح هشوف العكارة باللون الأحمر يبقى Tetrazolium chloride يعتبر color indicator هذا مصطلح مهم ملاحظ في الصورة انه انا هان اتلونت كل ال tube باللون الأحمر يبقى العكارة موجودة على خط الزراع وعلى اتحركت بعيدا عن خط الزراع يبقى هي positive لكن هان فقط", "tokens": [39237, 10943, 1975, 11242, 33911, 3224, 15844, 2423, 3021, 6225, 8592, 7649, 1975, 8592, 38688, 16472, 2304, 2407, 18863, 4117, 9640, 3660, 4724, 8592, 28820, 4032, 46958, 5016, 8032, 8592, 38688, 18863, 4117, 9640, 3660, 20666, 1211, 11536, 16247, 5016, 29973, 7251, 3555, 4587, 7578, 31580, 30695, 401, 2197, 35434, 7251, 34268, 26890, 2017, 16961, 23758, 3714, 9381, 9566, 1211, 5016, 3714, 16095, 3714, 15040, 5016, 19913, 8978, 31767, 13063, 3660, 16472, 3224, 1975, 8315, 8032, 7649, 1975, 2655, 1211, 11536, 2655, 28242, 2423, 9917, 20666, 1211, 11536, 16247, 5016, 29973, 7251, 3555, 4587, 7578, 18863, 4117, 9640, 3660, 3714, 29245, 23328, 3660, 15844, 16490, 9566, 6024, 110, 23557, 3615, 4032, 3615, 23942, 1975, 2655, 5016, 31747, 2655, 45030, 25708, 995, 18871, 16490, 9566, 6024, 110, 23557, 3615, 7251, 3555, 4587, 7578, 39896, 3353, 44381, 8032, 7649, 6156, 47432], "avg_logprob": -0.1466517876301493, "compression_ratio": 1.8131868131868132, "no_speech_prob": 7.474422454833984e-05, "words": [{"start": 1854.86, "end": 1855.28, "word": "تمام", "probability": 0.59844970703125}, {"start": 1855.28, "end": 1855.8, "word": " اضيفه", "probability": 0.8720703125}, {"start": 1855.8, "end": 1855.98, "word": " على", "probability": 0.81005859375}, {"start": 1855.98, "end": 1856.14, "word": " ال", "probability": 0.8779296875}, {"start": 1856.14, "end": 1856.38, "word": " media", "probability": 0.8681640625}, {"start": 1856.38, "end": 1856.76, "word": " عشان", "probability": 0.9571940104166666}, {"start": 1856.76, "end": 1857.24, "word": " اشوف", "probability": 0.947265625}, {"start": 1857.24, "end": 1857.8, "word": " انمو", "probability": 0.7646484375}, {"start": 1857.8, "end": 1858.36, "word": " العكارة", "probability": 0.9234619140625}, {"start": 1858.36, "end": 1858.9, "word": " بشكل", "probability": 0.9807942708333334}, {"start": 1858.9, "end": 1859.36, "word": " واضح", "probability": 0.9677734375}, {"start": 1859.36, "end": 1859.76, "word": " هشوف", "probability": 0.8942057291666666}, {"start": 1859.76, "end": 1860.36, "word": " العكارة", "probability": 0.9891357421875}, {"start": 1860.36, "end": 1860.88, "word": " باللون", "probability": 0.9788411458333334}, {"start": 1860.88, "end": 1861.42, "word": " الأحمر", "probability": 0.9832356770833334}, {"start": 1861.42, "end": 1861.96, "word": " يبقى", "probability": 0.9825439453125}, {"start": 1861.96, "end": 1863.0, "word": " Tetrazolium", "probability": 0.57763671875}, {"start": 1863.0, "end": 1863.56, "word": " chloride", "probability": 0.2467041015625}, {"start": 1863.56, "end": 1864.18, "word": " يعتبر", "probability": 0.9469401041666666}, {"start": 1864.18, "end": 1864.56, "word": " color", "probability": 0.67626953125}, {"start": 1864.56, "end": 1865.2, "word": " indicator", "probability": 0.970703125}, {"start": 1865.2, "end": 1865.42, "word": " هذا", "probability": 0.85009765625}, {"start": 1865.42, "end": 1865.88, "word": " مصطلح", "probability": 0.99609375}, {"start": 1865.88, "end": 1866.32, "word": " مهم", "probability": 0.992919921875}, {"start": 1866.32, "end": 1867.56, "word": " ملاحظ", "probability": 0.79364013671875}, {"start": 1867.56, "end": 1867.66, "word": " في", "probability": 0.9404296875}, {"start": 1867.66, "end": 1868.12, "word": " الصورة", "probability": 0.9685872395833334}, {"start": 1868.12, "end": 1868.38, "word": " انه", "probability": 0.567626953125}, {"start": 1868.38, "end": 1868.72, "word": " انا", "probability": 0.67724609375}, {"start": 1868.72, "end": 1869.04, "word": " هان", "probability": 0.770263671875}, {"start": 1869.04, "end": 1869.94, "word": " اتلونت", "probability": 0.92763671875}, {"start": 1869.94, "end": 1870.4, "word": " كل", "probability": 0.95458984375}, {"start": 1870.4, "end": 1871.06, "word": " ال", "probability": 0.7802734375}, {"start": 1871.06, "end": 1871.8, "word": " tube", "probability": 0.16259765625}, {"start": 1871.8, "end": 1872.18, "word": " باللون", "probability": 0.85009765625}, {"start": 1872.18, "end": 1872.58, "word": " الأحمر", "probability": 0.9913736979166666}, {"start": 1872.58, "end": 1872.94, "word": " يبقى", "probability": 0.9854736328125}, {"start": 1872.94, "end": 1873.46, "word": " العكارة", "probability": 0.984130859375}, {"start": 1873.46, "end": 1874.04, "word": " موجودة", "probability": 0.9730224609375}, {"start": 1874.04, "end": 1874.24, "word": " على", "probability": 0.9609375}, {"start": 1874.24, "end": 1874.48, "word": " خط", "probability": 0.9921875}, {"start": 1874.48, "end": 1874.88, "word": " الزراع", "probability": 0.9620361328125}, {"start": 1874.88, "end": 1875.26, "word": " وعلى", "probability": 0.7430013020833334}, {"start": 1875.26, "end": 1876.24, "word": " اتحركت", "probability": 0.901953125}, {"start": 1876.24, "end": 1876.58, "word": " بعيدا", "probability": 0.962890625}, {"start": 1876.58, "end": 1876.76, "word": " عن", "probability": 0.978515625}, {"start": 1876.76, "end": 1877.02, "word": " خط", "probability": 0.99072265625}, {"start": 1877.02, "end": 1877.42, "word": " الزراع", "probability": 0.973388671875}, {"start": 1877.42, "end": 1877.68, "word": " يبقى", "probability": 0.9410400390625}, {"start": 1877.68, "end": 1877.92, "word": " هي", "probability": 0.6923828125}, {"start": 1877.92, "end": 1878.56, "word": " positive", "probability": 0.87255859375}, {"start": 1878.56, "end": 1879.7, "word": " لكن", "probability": 0.93408203125}, {"start": 1879.7, "end": 1880.18, "word": " هان", "probability": 0.937744140625}, {"start": 1880.18, "end": 1880.66, "word": " فقط", "probability": 0.997314453125}], "temperature": 1.0}, {"id": 71, "seek": 189891, "start": 1880.87, "end": 1898.91, "text": "اللي العكارة فقط كانت على خط الزراعة اللي اتلون باللون الأحمر خط الزراعة يبقى non motile هنبقى نلاحظ انه اتحركت ايضا بعيد عن خط الزراعة يبقى هي positive هك بنكون خلصنا ال bacterial motility السلام عليكم ورحمة الله وبركاته", "tokens": [6027, 20292, 18863, 4117, 9640, 3660, 6156, 47432, 25961, 2655, 15844, 16490, 9566, 6024, 110, 23557, 3615, 3660, 13672, 1829, 1975, 2655, 1211, 11536, 20666, 1211, 11536, 16247, 5016, 29973, 16490, 9566, 6024, 110, 23557, 3615, 3660, 7251, 3555, 4587, 7578, 2107, 2184, 794, 8032, 1863, 3555, 4587, 7578, 8717, 15040, 5016, 19913, 16472, 3224, 1975, 2655, 5016, 31747, 2655, 1975, 1829, 11242, 995, 45030, 25708, 18871, 16490, 9566, 6024, 110, 23557, 3615, 3660, 7251, 3555, 4587, 7578, 39896, 3353, 8032, 4117, 44945, 30544, 16490, 1211, 9381, 8315, 2423, 35632, 2184, 1140, 21136, 37440, 25894, 24793, 4032, 2288, 35571, 3660, 21984, 4032, 26890, 4117, 9307, 3224], "avg_logprob": -0.16734812387796205, "compression_ratio": 1.7535545023696681, "no_speech_prob": 0.0002034902572631836, "words": [{"start": 1880.87, "end": 1881.37, "word": "اللي", "probability": 0.321533203125}, {"start": 1881.37, "end": 1882.27, "word": " العكارة", "probability": 0.924560546875}, {"start": 1882.27, "end": 1882.61, "word": " فقط", "probability": 0.9736328125}, {"start": 1882.61, "end": 1882.99, "word": " كانت", "probability": 0.988525390625}, {"start": 1882.99, "end": 1883.15, "word": " على", "probability": 0.90234375}, {"start": 1883.15, "end": 1883.55, "word": " خط", "probability": 0.99560546875}, {"start": 1883.55, "end": 1884.15, "word": " الزراعة", "probability": 0.810595703125}, {"start": 1884.15, "end": 1884.27, "word": " اللي", "probability": 0.828369140625}, {"start": 1884.27, "end": 1884.63, "word": " اتلون", "probability": 0.8072509765625}, {"start": 1884.63, "end": 1884.99, "word": " باللون", "probability": 0.9627278645833334}, {"start": 1884.99, "end": 1885.45, "word": " الأحمر", "probability": 0.95751953125}, {"start": 1885.45, "end": 1885.83, "word": " خط", "probability": 0.9501953125}, {"start": 1885.83, "end": 1886.33, "word": " الزراعة", "probability": 0.89912109375}, {"start": 1886.33, "end": 1886.67, "word": " يبقى", "probability": 0.94287109375}, {"start": 1886.67, "end": 1886.87, "word": " non", "probability": 0.78759765625}, {"start": 1886.87, "end": 1887.57, "word": " motile", "probability": 0.6566162109375}, {"start": 1887.57, "end": 1888.41, "word": " هنبقى", "probability": 0.6580078125}, {"start": 1888.41, "end": 1888.83, "word": " نلاحظ", "probability": 0.970458984375}, {"start": 1888.83, "end": 1889.11, "word": " انه", "probability": 0.6279296875}, {"start": 1889.11, "end": 1889.61, "word": " اتحركت", "probability": 0.97685546875}, {"start": 1889.61, "end": 1889.89, "word": " ايضا", "probability": 0.894775390625}, {"start": 1889.89, "end": 1890.19, "word": " بعيد", "probability": 0.974609375}, {"start": 1890.19, "end": 1890.31, "word": " عن", "probability": 0.331787109375}, {"start": 1890.31, "end": 1890.65, "word": " خط", "probability": 0.97705078125}, {"start": 1890.65, "end": 1891.15, "word": " الزراعة", "probability": 0.95146484375}, {"start": 1891.15, "end": 1891.47, "word": " يبقى", "probability": 0.9456787109375}, {"start": 1891.47, "end": 1891.71, "word": " هي", "probability": 0.4580078125}, {"start": 1891.71, "end": 1892.57, "word": " positive", "probability": 0.896484375}, {"start": 1892.57, "end": 1893.79, "word": " هك", "probability": 0.58837890625}, {"start": 1893.79, "end": 1894.15, "word": " بنكون", "probability": 0.80517578125}, {"start": 1894.15, "end": 1894.77, "word": " خلصنا", "probability": 0.986328125}, {"start": 1894.77, "end": 1895.01, "word": " ال", "probability": 0.962890625}, {"start": 1895.01, "end": 1895.55, "word": " bacterial", "probability": 0.802734375}, {"start": 1895.55, "end": 1896.29, "word": " motility", "probability": 0.944091796875}, {"start": 1896.29, "end": 1897.07, "word": " السلام", "probability": 0.908447265625}, {"start": 1897.07, "end": 1897.53, "word": " عليكم", "probability": 0.95263671875}, {"start": 1897.53, "end": 1897.95, "word": " ورحمة", "probability": 0.9619140625}, {"start": 1897.95, "end": 1898.19, "word": " الله", "probability": 0.9716796875}, {"start": 1898.19, "end": 1898.91, "word": " وبركاته", "probability": 0.99013671875}], "temperature": 1.0}], "language": "ar", "language_probability": 1.0, "duration": 1900.39075, "duration_after_vad": 1837.290624999996}
\ No newline at end of file
diff --git a/PL9fwy3NUQKwZWgAb_V1ATlRRsH-6i3cPC/DBNaI7FWU-U.srt b/PL9fwy3NUQKwZWgAb_V1ATlRRsH-6i3cPC/DBNaI7FWU-U.srt
new file mode 100644
index 0000000000000000000000000000000000000000..d878614b6104f1dfd46b694ea8edb50e1331be31
--- /dev/null
+++ b/PL9fwy3NUQKwZWgAb_V1ATlRRsH-6i3cPC/DBNaI7FWU-U.srt
@@ -0,0 +1,1146 @@
+
+1
+00:00:00,470 --> 00:00:04,270
+Morphology of bacteria; the basis for studying is
+
+2
+00:00:04,270 --> 00:00:08,070
+to study the morphology and structure of bacteria
+
+3
+00:00:08,070 --> 00:00:11,990
+قلنا هدفي من الصباغة أني أنا أتعرف على شكل
+
+4
+00:00:11,990 --> 00:00:16,170
+البكتيريا وقلنا أشكال البكتيريا ممكن تكون
+
+5
+00:00:16,170 --> 00:00:21,550
+spherical، كروية، coccus or cocci، ممكن تكون rod shape،
+
+6
+00:00:21,550 --> 00:00:26,090
+اللي هي عصوية، bacillus or bacilli، يبقى أشكال
+
+7
+00:00:26,090 --> 00:00:30,470
+البكتيريا ممكن تكون cocci وممكن تكون bacilli، قلنا
+
+8
+00:00:30,470 --> 00:00:33,570
+على الـ simple stain هدفي أني أنا أتعرف على الشكل
+
+9
+00:00:33,570 --> 00:00:37,650
+و الـ arrangement للبكتيريا، اتعرفنا على الشكل of
+
+10
+00:00:37,650 --> 00:00:40,690
+البكتيريا، طب شو بالنسبة للـ arrangement؟ الـ
+
+11
+00:00:40,690 --> 00:00:45,210
+arrangement هو ترتيب الخلايا البكتيرية، بيعتمد الـ
+
+12
+00:00:45,210 --> 00:00:48,730
+arrangement على الـ plane of division، على مستوى
+
+13
+00:00:48,730 --> 00:00:52,990
+الانقسام seen in the organism. Bacteria may also
+
+14
+00:00:52,990 --> 00:00:58,660
+have a specific arrangement of the cells. المستوى
+
+15
+00:00:58,660 --> 00:01:04,420
+الانقسام، عشان نفهم أكثر، لو انقسمت الخلية البكتيرية
+
+16
+00:01:04,420 --> 00:01:08,440
+وكان مستوى الانقسام، إذا ملاحظين، كان vertical بشكل
+
+17
+00:01:08,440 --> 00:01:14,420
+عمودي، وعطى خليتين، هدول الخليتين ديل ملتصقين مع
+
+18
+00:01:14,420 --> 00:01:19,120
+بعض، ما انفصلوا، وكان الشكل بالأصل كروي، يبقى بسمي الـ
+
+19
+00:01:19,120 --> 00:01:24,460
+arrangement دبلو، بما أن الشكل كروي يبقى دبلو كوكاي.
+
+20
+00:01:24,460 --> 00:01:29,940
+لو كان الشكل في الأصل عصوي، يبقى بسمي الآخر شيء
+
+21
+00:01:29,940 --> 00:01:34,200
+البكتيريا diplo bacillus، diplo الـ arrangement وbacillus
+
+22
+00:01:34,200 --> 00:01:40,380
+الشكل. طيب لو كان مستوى الانقسام بشكل عمودي لكن كان
+
+23
+00:01:40,380 --> 00:01:45,700
+continuous،  مستمر، مش فقط خليتين، كان كوّن chain
+
+24
+00:01:45,700 --> 00:01:50,360
+of coccal cells، كوّن سلسلة من الخلايا البكتيرية، و
+
+25
+00:01:50,360 --> 00:01:54,740
+كانت الشكل في الأصل كروي، يبقى بسمي الـ arrangement
+
+26
+00:01:54,740 --> 00:01:59,160
+strepto، والشكل كروي، يبقى بالنهاية اسمها
+
+27
+00:01:59,160 --> 00:02:03,700
+streptococci. طب لو كان الشكل في الأصل عصوي، وكانت
+
+28
+00:02:03,700 --> 00:02:08,580
+انقسمت بشكل عمودي لكن ظلت continually، بسميها
+
+29
+00:02:08,580 --> 00:02:13,380
+streptobacillary. بالنسبة للـ plane of division، لو
+
+30
+00:02:13,380 --> 00:02:17,320
+كان عندي مستوى الانقسام vertical و horizontal، كان
+
+31
+00:02:17,320 --> 00:02:23,470
+في عندي طول وعرض، بسمي الشكل tetrad. لو كان عندي
+
+32
+00:02:23,470 --> 00:02:28,190
+مستوى الانقسام ثلاثة: طول، وعرض، وارتفاع، بسمي الـ
+
+33
+00:02:28,190 --> 00:02:32,270
+arrangement sarcina. احنا بنعرف أو قلنا في معمل
+
+34
+00:02:32,270 --> 00:02:37,450
+الـ microscope، الصورة اللي بيعطيني إياها الـ light
+
+35
+00:02:37,450 --> 00:02:41,490
+microscope هي virtual، صورة افتراضية، بتعطيني فقط
+
+36
+00:02:41,490 --> 00:02:45,690
+طول وعرض. فالـ sarcina، أنا فيه عندي مستوى الانقسام
+
+37
+00:02:45,690 --> 00:02:49,790
+كان طول وعرض وارتفاع، يبقى ما بقدر أتعرف على
+
+38
+00:02:49,790 --> 00:02:53,810
+الساركينة في الـ light microscope، بحتاج إلى المشرط
+
+39
+00:02:53,810 --> 00:02:59,590
+التشريحي حتى أني أنا أشوف الساركينة. لو انقسمت بشكل
+
+40
+00:02:59,590 --> 00:03:04,510
+randomly، بشكل عشوائي، بسميها staphylococcus. ولو
+
+41
+00:03:04,510 --> 00:03:08,970
+كان الشكل كروي، بسميها staphylococcus. بشكل عنقودي
+
+42
+00:03:08,970 --> 00:03:12,770
+للعينة، بنسميها عنقودي. ممكن يكون ما عندي
+
+43
+00:03:12,770 --> 00:03:15,770
+arrangement، تظلها single، سواء single كوكاي أو
+
+44
+00:03:15,770 --> 00:03:19,660
+single bacillus. بنحكي non-arrangement. يبقى هذا
+
+45
+00:03:19,660 --> 00:03:23,280
+بالنسبة للـ arrangement of bacteria، نرجع على الـ
+
+46
+00:03:23,280 --> 00:03:27,160
+slide. Diplococci are formed when the plane of
+
+47
+00:03:27,160 --> 00:03:30,280
+division is vertical. بقولنا بتتكون إذا كان مستوى
+
+48
+00:03:30,280 --> 00:03:34,420
+الانقسام عمودي، والنتيجة كان عندي two coccal cells،
+
+49
+00:03:34,420 --> 00:03:38,980
+خليتين كرويتين لم ينفصلوا، don't completely
+
+50
+00:03:38,980 --> 00:03:43,000
+separate from each other، ما انفصلوا عن بعض. بقولنا
+
+51
+00:03:43,000 --> 00:03:45,640
+if the cell divides in the vertical plane
+
+52
+00:03:45,640 --> 00:03:49,660
+continually and the cells don't separate، لو كان
+
+53
+00:03:49,660 --> 00:03:54,140
+مستوى الانقسام عمودي لكن بشكل مستمر، وهذه الخلايا ما
+
+54
+00:03:54,140 --> 00:03:58,020
+انفصلت، وكونت chain of coccal cells، بسمي أنا
+
+55
+00:03:58,020 --> 00:04:02,820
+الخلايا streptococci أو streptobacilli حسب الشكل.
+
+56
+00:04:02,820 --> 00:04:06,740
+Other arrangements، لو انقسمت بشكل رباعي، بسميها
+
+57
+00:04:06,740 --> 00:04:10,870
+tetrad. كان مستوى الانقسام vertical و horizontal. لو
+
+58
+00:04:10,870 --> 00:04:14,910
+كان مستوى الانقسام طول، وعرض، وارتفاع، بشكل ثماني،
+
+59
+00:04:14,910 --> 00:04:18,010
+بسميه sarcina، وقولنا الساركينة ما بشوفها في الـ
+
+60
+00:04:18,010 --> 00:04:21,990
+light microscope. آخر شيء، لو كان بشكل randomly أو
+
+61
+00:04:21,990 --> 00:04:26,410
+cluster، بسمي الـ arrangement staphylococcus، كروي، يبقى هذا
+
+62
+00:04:26,410 --> 00:04:31,750
+بالنسبة ليه الـ arrangement، الـ simple stain بتعرف
+
+63
+00:04:31,750 --> 00:04:34,890
+من خلال الشكل والـ arrangement، الشكل ممكن يكون
+
+64
+00:04:34,890 --> 00:04:38,110
+كروي، عصوي، الـ arrangement حسب مستوى الانقسام،
+
+65
+00:04:38,110 --> 00:04:42,960
+إذا كان vertical: diplo، vertical continually: strepto،
+
+66
+00:04:42,960 --> 00:04:46,220
+randomly: بشكل عشوائي بتكون staphylococcus، ممكن يكون no
+
+67
+00:04:46,220 --> 00:04:50,480
+arrangement، ممكن يكون other زي الـ tetrad والساركي،
+
+68
+00:04:50,480 --> 00:04:56,320
+هذا بالنسبة للشكل والـ arrangement. خلصنا من قلنا أو
+
+69
+00:04:56,320 --> 00:05:00,060
+اتكلمنا على الهدف أو الـ principle من الـ simple
+
+70
+00:05:00,060 --> 00:05:03,400
+stain. الـ objective، هدف الـ simple stain. هلأ نيجي
+
+71
+00:05:03,400 --> 00:05:08,290
+لخطوات العمل، أشهر الـ procedure اللي أنا بشتغلها أو
+
+72
+00:05:08,290 --> 00:05:12,350
+الخطوات حتى أشتغل الـ simple staining
+
+73
+00:05:12,350 --> 00:05:16,350
+protocol in microbiology lab. أول خطوة اللي هي الـ
+
+74
+00:05:16,350 --> 00:05:20,990
+bacterial smear preparation. بدي أحضر البكتيريا،
+
+75
+00:05:20,990 --> 00:05:25,490
+بقولنا تحضير البكتيريا بيعتمد على نوع الوسط اللي
+
+76
+00:05:25,490 --> 00:05:29,850
+نمت عليه البكتيريا. ممكن تنمو على ميديا، وسط، هذا
+
+77
+00:05:29,850 --> 00:05:34,670
+الوسط ممكن يكون agar، agar يعني صلبة، وقتها أنا بصب
+
+78
+00:05:34,670 --> 00:05:39,950
+الـ media في بلات، في أطباق بتري، في بلات، وممكن تكون
+
+79
+00:05:39,950 --> 00:05:44,230
+سائلة، liquid، أو بنسميها broth، broth يعني liquid
+
+80
+00:05:44,230 --> 00:05:49,390
+سائل، ووقتها بصبها في tube. طريقة تحضير البكتيريا
+
+81
+00:05:49,390 --> 00:05:53,790
+بتختلف حسب إذا كان الوسط agar أو كان broth. لو كان
+
+82
+00:05:53,790 --> 00:05:58,490
+agar، طبعا أكيد هأصبه في بلات، بجيب الـ slide وبضيف
+
+83
+00:05:58,490 --> 00:06:01,950
+drop من الـ normal saline، الـ normal saline محلول
+
+84
+00:06:01,950 --> 00:06:08,790
+ملحي، تركيزه 8.5% من 100%، زائد loop full of bacteria.
+
+85
+00:06:08,790 --> 00:06:14,210
+بأخذ مسحة من الكولوني من الطبق، باستيرايل loop، مع
+
+86
+00:06:14,210 --> 00:06:17,990
+أن أنا أستخدم استيرايل loop، تكون aseptic technique،
+
+87
+00:06:17,990 --> 00:06:22,550
+أمنع الـ contamination. لو كان nichrome loop عندي، بتم
+
+88
+00:06:22,550 --> 00:06:27,570
+تعقيمه باستخدام اللهب. كاتبينها، من ضمن النصائح
+
+89
+00:06:27,570 --> 00:06:31,370
+اللي هو يفضل أني أنا آخذ isolated colony. ليش
+
+90
+00:06:31,370 --> 00:06:35,270
+isolated colony؟ عشان أضمن أن كل البكتيريا أصلها
+
+91
+00:06:35,270 --> 00:06:39,870
+واحدة، بالتالي هيكون شكلها واحد. بعمل، بعد ما أخذت
+
+92
+00:06:39,870 --> 00:06:42,870
+الـ loop full of bacteria، بعمل mix مع الـ normal
+
+93
+00:06:42,870 --> 00:06:48,130
+saline، وبروح بفرده بالـ loop وبحاول أني أنا أفرده
+
+94
+00:06:48,130 --> 00:06:53,790
+على كل الـ slide، تمام؟ هيك أنا خلصت من أول طريقة اللي
+
+95
+00:06:53,790 --> 00:06:58,920
+هي الـ smear preparation. طيب أنا قلت بدي أستخدم أو
+
+96
+00:06:58,920 --> 00:07:02,680
+أني أنا لازم آخذ isolated colony. ايش هي الـ
+
+97
+00:07:02,680 --> 00:07:05,960
+isolated colony؟ أنا في عندي أكثر من طريقة لزراعة،
+
+98
+00:07:05,960 --> 00:07:10,060
+هنتعرف عليها في معمل، الـ media preparation، ضمن طرق
+
+99
+00:07:10,060 --> 00:07:13,800
+الزراعة، طريقة الـ quadrant، طريقة الأربع أرباع، هاي
+
+100
+00:07:13,800 --> 00:07:17,740
+الطريقة، الهدف منها أحصل على isolated colony، أنا
+
+101
+00:07:17,740 --> 00:07:21,400
+بزرع الربع الأول، تمام، بجيب البكتيريا اللي أنا بدي
+
+102
+00:07:21,400 --> 00:07:28,070
+أزرعها باستخدام الـ loop، وبزرع في الربع الأول، بعدها
+
+103
+00:07:28,070 --> 00:07:31,070
+بستخدم الـ loop تاني، إذا كنت بستخدم الـ plastic loop،
+
+104
+00:07:31,070 --> 00:07:34,970
+بستخدم loop جديد، وإذا بستخدم nichrome loop بروح
+
+105
+00:07:34,970 --> 00:07:39,930
+بعقم هذا الـ loop، ما برجع بأخذ بكتيريا ثانية، فقط بأخذ
+
+106
+00:07:39,930 --> 00:07:46,490
+من وين بأخذ؟ من هذا الربع، وبطلع بخطين، هيك أنا في
+
+107
+00:07:46,490 --> 00:07:51,530
+هذه الطريقة بكون خففته. بعدها بستخدم loop جديد، إذا
+
+108
+00:07:51,530 --> 00:07:55,410
+plastic، وبزرع الربع الثالث، وهكذا في الرابع، الرابع،
+
+109
+00:07:55,410 --> 00:07:59,550
+بهذه الطريقة بخفف الـ colonies، بحاول أعزل الـ colonies
+
+110
+00:07:59,550 --> 00:08:02,790
+عن بعض، وأحصل على isolated colonies. في هون دي أنا
+
+111
+00:08:02,790 --> 00:08:06,930
+صورة، هذه الصورة، إذا ملاحظين، الربع الأول ثقيل، و
+
+112
+00:08:06,930 --> 00:08:10,350
+المستعمرات فوق بعض، الربع الثاني أخف من الربع الأول،
+
+113
+00:08:10,350 --> 00:08:14,560
+بدأت شوية المستعمرات تتبعد عن بعض، الربع الثالث أيضا
+
+114
+00:08:14,560 --> 00:08:18,040
+أخف من الثاني، في الرابع، نلاحظ أنه الـ colonies
+
+115
+00:08:18,040 --> 00:08:21,740
+معزولة عن بعض، فأنا بأخذ هاي colonies، هيك بضمن أن
+
+116
+00:08:21,740 --> 00:08:26,220
+أصلها خلية بكتيريا واحدة، يعني كل شكل الخلايا هيكون
+
+117
+00:08:26,220 --> 00:08:30,120
+نفس الشيء. طيب ايش بالنسبة للـ normal saline؟
+
+118
+00:08:30,120 --> 00:08:33,500
+اتكلمنا عن البكتيريا، نيجي نحكي على الـ normal
+
+119
+00:08:33,500 --> 00:08:37,680
+saline، الـ normal saline لازم يكون تركيزه 8.5% من
+
+120
+00:08:37,680 --> 00:08:43,500
+100%. كيف بدي أحضر هذا التركيز، أو ايش معنى 8.5 من 100
+
+121
+00:08:43,500 --> 00:08:48,900
+%? معنى أنه 8.5 من 100 جرام من الـ normal saline هو
+
+122
+00:08:48,900 --> 00:08:53,700
+بيكون salt، زي الملح، مذوبة في 100 مل من الـ
+
+123
+00:08:53,700 --> 00:08:57,720
+distilled water. طيب لو أنا بدي كمية أكثر، بروح بدرب
+
+124
+00:08:57,720 --> 00:09:02,700
+باستخدام التناسب، وليه؟ على سبيل المثال، بدي 1000 مل، بأخذ
+
+125
+00:09:02,700 --> 00:09:07,100
+8.5 جرام من الـ normal saline، وبذوبها بـ 1000 مل من
+
+126
+00:09:07,100 --> 00:09:12,290
+الـ distilled water. ننتبه هون إنه أنا هذا الـ normal
+
+127
+00:09:12,290 --> 00:09:15,490
+saline صلب، فأنا استخدمت هذه الطريقة. لكن لما كنا
+
+128
+00:09:15,490 --> 00:09:22,510
+نحضر كحول 70%، بحط 70 مل كحول، وبكمل على الـ 100 مل بـ distilled water. يعني بالنهاية بيطلع الـ distilled water
+
+129
+00:09:22,510 --> 00:09:26,450
+ثلاثين، لكن هون أنا حطيت المية كلها distilled water
+
+130
+00:09:26,450 --> 00:09:29,910
+لأنه الـ normal saline هون صلب. طيب، شو وظيفة الـ
+
+131
+00:09:29,910 --> 00:09:34,910
+normal saline؟ احنا كاتبينها، إن normal saline
+
+132
+00:09:34,910 --> 00:09:37,450
+بيحافظ لي على الضغط الأسموزي، لأنه لو كان الـ
+
+133
+00:09:37,450 --> 00:09:41,030
+normal saline أكثر من 8.5% من 100%، هيصير في عندي
+
+134
+00:09:41,030 --> 00:09:45,550
+shrinking للخلايا، لو كان أقل من 8.5% من 100% هيصير
+
+135
+00:09:49,950 --> 00:09:55,210
+في عندي swelling للخلايا. سبب ثاني إن أنا استخدمت
+
+136
+00:09:55,210 --> 00:09:58,270
+الـ normal saline في هذه الطريقة، لأن الكولوني
+
+137
+00:09:58,270 --> 00:10:02,510
+بتكون جافة، فأنا بدي شيء سائل، أني أحاول أوزعها على
+
+138
+00:10:02,510 --> 00:10:05,690
+الـ slide. يبقى عشان يحافظ لي على الـ osmotic
+
+139
+00:10:05,690 --> 00:10:09,730
+pressure، وسبب ثاني لأن الكولوني جافة، فبدي شيء
+
+140
+00:10:09,730 --> 00:10:14,510
+أحاول يسهل عليّ توزيع الخلايا البكتيرية على الـ
+
+141
+00:10:14,510 --> 00:10:18,590
+slide. هيك خلصنا من طريقة الـ agar. طيب لو كان الوسط
+
+142
+00:10:18,590 --> 00:10:22,780
+الـ broth، لو كان الوسط سائل، بقولنا مش محتاجة أنا
+
+143
+00:10:22,780 --> 00:10:27,500
+normal saline، لأنه هو سائل، فقط بضيف drop من الـ
+
+144
+00:10:27,500 --> 00:10:30,980
+bacterial broth أو الـ bacterial suspension، نفس
+
+145
+00:10:30,980 --> 00:10:33,780
+المعنى، الـ bacterial broth نفس المعنى الـ bacterial
+
+146
+00:10:33,780 --> 00:10:38,180
+suspension. bacterial broth معناها ميديا سائلة
+
+147
+00:10:38,180 --> 00:10:42,220
+مزروعة فيها بكتيريا. bacterial suspension اللي هو
+
+148
+00:10:42,220 --> 00:10:47,100
+سائل، ممكن يكون normal saline، ممكن يكون ميديا سائلة
+
+149
+00:10:47,100 --> 00:10:51,310
+معلقة فيه بكتيريا، وبفرد باستخدام الـ loop، أو في الأصل
+
+150
+00:10:51,310 --> 00:10:54,410
+ممكن ما آخذ، ما آخذ في الـ dropper، آخذ الـ bacterial
+
+151
+00:10:54,410 --> 00:10:58,290
+suspension باستخدام الـ loop، أحاول أفرد حوليها على الـ slide.
+
+152
+00:10:58,290 --> 00:11:02,930
+هان بهذه الطريقة ما بحط cover slip. ننتبه في طريقة
+
+153
+00:11:02,930 --> 00:11:06,410
+الـ wet mount، هديك الـ living، understand الـ organism،
+
+154
+00:11:06,410 --> 00:11:09,670
+بنستخدم الـ wet mount، بنستخدم الـ cover slip. هان ما
+
+155
+00:11:09,670 --> 00:11:14,290
+بنحط cover slip. هيك خلصنا من أول خطوة اللي هي الـ
+
+156
+00:11:14,290 --> 00:11:18,630
+bacterial smear preparation. لكن في بعض الأحيان ممكن
+
+157
+00:11:18,630 --> 00:11:23,190
+يصير في عندي مشاكل في السمير، ممكن السمير يكون عندي
+
+158
+00:11:23,190 --> 00:11:27,210
+thick، سميك، وممكن يكون thin، رقيق. طب هي مشكلة؟ اه
+
+159
+00:11:27,210 --> 00:11:32,790
+مشكلة، أكيد. thick smear، لو كان أنا استخدمت أو عملت
+
+160
+00:11:32,790 --> 00:11:36,270
+thick smear، يعني هتكون الخلايا أو الخلايا البكتيرية
+
+161
+00:11:36,270 --> 00:11:39,950
+فوق بعض، فبالتالي مش هتعرفي تميزي الشكل والـ
+
+162
+00:11:39,950 --> 00:11:44,860
+arrangement. عشان نحل هذه المشكلة، منضيف ومنخفف،
+
+163
+00:11:44,860 --> 00:11:48,620
+مايعني منخفف؟ يعني منضيف another drop of normal
+
+164
+00:11:48,620 --> 00:11:54,220
+saline. thin smear، يعني الخلايا بتكون قليلة في هذا الـ
+
+165
+00:11:54,220 --> 00:11:57,860
+slide. عشان أني أنا أحل هذه المشكلة، شو بعمل؟ take
+
+166
+00:11:57,860 --> 00:12:01,500
+another loop full of bacteria، بضيف مسحة أخرى من
+
+167
+00:12:01,500 --> 00:12:04,340
+البكتيريا، يبقى خلصنا أول خطوة اللي هي الـ
+
+168
+00:12:04,340 --> 00:12:08,560
+bacterial smear preparation. الخطوة الثانية، الـ air
+
+169
+00:12:08,560 --> 00:12:13,350
+drying، بنخليها تنشف الـ slide في الهواء، أو ممكن
+
+170
+00:12:13,350 --> 00:12:17,450
+نحطها في الـ incubator 37 درجة سيليزية، بيجففها
+
+171
+00:12:17,450 --> 00:12:22,190
+بشكل أسرع. طب ليش أنا بدي أعمل هذه الخطوة؟ بقولنا
+
+172
+
+223
+00:15:53,720 --> 00:15:56,520
+هو الـ basic stain سواء استخدمت ميثيلين بلو
+
+224
+00:15:56,520 --> 00:16:01,420
+كاربولفوكسين، سفرانين، أي basic stain  بستخدم لمدة
+
+225
+00:16:01,420 --> 00:16:04,420
+معينة، بعد ما تنتهي هذه المدة بعمل washing بالـ
+
+226
+00:16:04,420 --> 00:16:08,460
+distilled water أو tap water، بعدها، ماذا أضيف؟
+
+227
+00:16:08,460 --> 00:16:11,340
+كما قلنا في الجدول، ممكن أضيف mordant، المثبت
+
+228
+00:16:11,340 --> 00:16:15,480
+الأيضائي، بعدها بغسل، نفس الشيء، بعمل washing هذه
+
+229
+00:16:15,480 --> 00:16:18,040
+بالنسبة لخطوات الـ smear stain
+
+230
+00:16:22,100 --> 00:16:26,400
+سنمر على السلايدات بشكل سريع لأن تقريبًا الـ
+
+231
+00:16:26,400 --> 00:16:31,200
+سلايدتان القادمتان لخصتا كل الموضوع، سنعرف الـ
+
+232
+00:16:31,200 --> 00:16:34,700
+سمير "smear" is a distribution of bacterial cells
+
+233
+00:16:34,700 --> 00:16:39,140
+on a slide for the purpose of viewing them under
+
+234
+00:16:39,140 --> 00:16:42,720
+the microscope، يبقى هنا عندي تعريف مهم جدًا، وهو
+
+235
+00:16:42,720 --> 00:16:46,900
+السمير، هو توزيع الخلايا البكتيرية على الـ
+
+236
+00:16:46,900 --> 00:16:50,340
+سلايد، بحيث أني أنا أقدر أشوفها تحت الميكروسكوب
+
+237
+00:16:51,220 --> 00:16:54,660
+الطريقة، أول شيء، أسيبتيكالي، ماذا يعني أسيبتيكالي؟
+
+238
+00:16:54,660 --> 00:16:58,420
+أسيبتيك تكنيك هي طرق، كل الطرق، لمنع الـ
+
+239
+00:16:58,420 --> 00:17:02,120
+contamination، من أني أستخدم sterile loops، أستخدم
+
+240
+00:17:02,120 --> 00:17:04,600
+sterile normal saline، الـ normal saline اللي أنا
+
+241
+00:17:04,600 --> 00:17:07,060
+أريد استخدامه، يجب أن يكون معقمًا، عقمه في الـ
+
+242
+00:17:07,060 --> 00:17:10,740
+autoclave، قبل هذا كله، يجب أني أنا أعقم الـ bench
+
+243
+00:17:10,740 --> 00:17:14,180
+الذي سأشتغل عليه، كذلك، الشغل يجب أن يكون في منطقة
+
+244
+00:17:14,180 --> 00:17:17,420
+معقمة، ممكن أني أنا أشتغل جانب اللهب أو بالـseptic
+
+245
+00:17:17,420 --> 00:17:21,470
+cabinet، كل هذه تسمى أسيبتيك تكنيك aseptically, a
+
+246
+00:17:21,470 --> 00:17:24,670
+small sample of the culture is spread over a slide
+
+247
+00:17:24,670 --> 00:17:26,890
+surface، هذه الخطوة هي الـ smearing preparation
+
+248
+00:17:26,890 --> 00:17:32,690
+أوزع العينة على السلايد، بعدها، أعرضها لـ، أعمل air
+
+249
+00:17:32,690 --> 00:17:36,370
+drying، حتى أمنع الـ lysis، ثالث خطوة، وهي الـ
+
+250
+00:17:36,370 --> 00:17:41,190
+fixation، وهذا كله بهدف to help the cells adhere to
+
+251
+00:17:41,190 --> 00:17:45,910
+the slide surface، آخر خطوة، وهي الـ staining، يبقى
+
+252
+00:17:45,910 --> 00:17:48,230
+هذه كل الخطوات التي ذكرناها
+
+253
+00:17:56,130 --> 00:18:01,230
+هنا في بعض الخطوات مرسومة، إنه استخدم اللي هو الـ
+
+254
+00:18:01,230 --> 00:18:04,930
+broth media، آخذ bacterial suspension أو bacterial
+
+255
+00:18:04,930 --> 00:18:09,230
+broth، باستخدام اللوب، أحاول أن أوزع، يصبح عالياً الـ
+
+256
+00:18:09,230 --> 00:18:15,350
+bacterial suspension على السلايد وأفردها، هنا، drop
+
+257
+00:18:15,350 --> 00:18:18,870
+of normal saline، آخذ لوب ممتلئ بكتيريا، full of bacterial
+
+258
+00:18:18,870 --> 00:18:22,650
+أحاول أن أعمل mix، وبعدها، يصير في عندي، بعد الـ air
+
+259
+00:18:22,650 --> 00:18:26,940
+drying، هكذا، نلاحظ أن الـ normal saline إذا لاحظتم، هو
+
+260
+00:18:26,940 --> 00:18:31,820
+شفاف، transparent، لما أنا أحط الكولوني، بيصير شكله
+
+261
+00:18:31,820 --> 00:18:36,620
+مثل milky appearance، مثل شكل الحليب، إذا تتذكرون، نحن
+
+262
+00:18:36,620 --> 00:18:39,580
+قلنا على الـ thick smear و thin smear، وكيف أنا أريد
+
+263
+00:18:39,580 --> 00:18:44,040
+أن أحل هذه المشكلة، اسمعوا، كيف أعرف إن والله
+
+264
+00:18:44,040 --> 00:18:47,640
+هل هو السمير thick أم thin؟ من خلال اللون، خلال
+
+265
+00:18:47,640 --> 00:18:50,760
+شغلي، إذا كان اللون كثيراً غامقاً، معناه أنه من الممكن
+
+266
+00:18:50,760 --> 00:18:54,150
+احتمال أن يكون السمير عندي thick، لو كان فاتح اللون
+
+267
+00:18:54,150 --> 00:18:57,790
+معناه احتمال أن يكون عندي thin smear، فأحاول أن أنا
+
+268
+00:18:57,790 --> 00:19:01,670
+أحل المشكلة، لو أنا بعد ما صبغته ويصير في عندي
+
+269
+00:19:01,670 --> 00:19:05,470
+drying، ممكن أني أعيد الشغل مرة ثانية، وأن أنا
+
+270
+00:19:05,470 --> 00:19:09,390
+أحاول أن أضيف كمية أكثر من البكتيريا أو من الـ
+
+271
+00:19:09,390 --> 00:19:12,250
+normal saline، حسب إذا كان thick أو thin smear
+
+272
+00:19:15,970 --> 00:19:20,550
+in this exercise we will use simple stain to show
+
+273
+00:19:20,550 --> 00:19:24,930
+the general structure of some bacteria، أقول لكم، الـ
+
+274
+00:19:24,930 --> 00:19:27,470
+simple stain التي أستخدمها، أني أنا أتعرف
+
+275
+00:19:27,470 --> 00:19:31,870
+على شكل البكتيريا، usually a single basic stain
+
+276
+00:19:31,870 --> 00:19:35,410
+قلنا، نستخدم فقط reagent واحد من الـ basic stain
+
+277
+00:19:35,410 --> 00:19:40,610
+في هذه الخطوة، simple stain لا يقدر... (repeated word "يقدر" removed)
+
+278
+00:19:40,610 --> 00:19:42,530
+(repeated word "يقدر" removed)
+
+279
+00:19:42,530 --> 00:19:44,250
+(repeated word "يقدر" removed)
+
+280
+00:19:44,250 --> 00:19:50,610
+(repeated word "يقدر" removed)
+
+281
+00:19:50,610 --> 00:19:53,350
+(repeated word "يقدر" removed)
+
+282
+00:19:53,350 --> 00:19:53,410
+(repeated word "يقدر" removed)
+
+283
+00:19:53,410 --> 00:19:56,650
+(repeated word "يقدر" removed)
+
+284
+00:20:07,320 --> 00:20:12,840
+They simply make the bacteria easier to see، فقط
+
+285
+00:20:12,840 --> 00:20:18,660
+هي تجعل البكتيريا سهلة رؤيتها، هذه الخطوات،
+
+286
+00:20:18,660 --> 00:20:24,000
+عندما، هو استخدم من broth media، using
+
+287
+00:20:24,000 --> 00:20:28,520
+inoculating loop، استخدم اللوب، وأكيد، طبعًا، لازم
+
+288
+00:20:28,520 --> 00:20:33,080
+ضروري يكون use sterile technique، smear the bacteria
+
+289
+00:20:33,080 --> 00:20:36,660
+أعمل، أفرد البكتيريا من الـ center حوالين، وبعدين
+
+290
+00:20:36,660 --> 00:20:40,180
+أحاول أني أنا أصل إلى الأطراف، if the bacterial
+
+291
+00:20:40,180 --> 00:20:43,480
+suspension is very thick، وتكلمنا عن هذا الموضوع
+
+292
+00:20:43,480 --> 00:20:47,060
+إذا كان thick، أضيف، add a drop of water، water
+
+293
+00:20:47,060 --> 00:20:50,540
+يقصد بها الـ normal saline، ثاني خطوة، وهي الـ
+
+294
+00:20:50,540 --> 00:20:55,660
+air-drying، كتب للسبب، to prevent lysis، ثالث خطوة،
+
+295
+00:20:55,660 --> 00:21:00,560
+الـ heat fixation، عن طريق تمرير، تمرير السلايد
+
+296
+00:21:00,560 --> 00:21:04,620
+على اللهب ثلاث مرات بسرعة، هذا يعمل partial
+
+297
+00:21:04,620 --> 00:21:07,440
+melting of the cell wall and membrane of bacteria
+
+298
+00:21:07,440 --> 00:21:12,220
+and make them stick to the slide، وتكلمنا عن هذا
+
+299
+00:21:12,220 --> 00:21:15,840
+الموضوع، don't overheat، لأنه يسبب destroy the
+
+300
+00:21:15,840 --> 00:21:22,290
+bacteria، آخر شيء، أريد أن أعمل خطوة الصباغة، أريد أن أغمر
+
+301
+00:21:22,290 --> 00:21:27,150
+السمير بالـ، الـ stain، basic stain، حسب المدة التي
+
+302
+00:21:27,150 --> 00:21:31,110
+مكتوبة لكل نوع من أنواع الصبغات، Carbol fuchsin,
+
+303
+00:21:31,210 --> 00:21:34,570
+methylene blue, nigrosin، إذا لاحظتم، أن الـ
+
+304
+00:21:34,570 --> 00:21:37,910
+carbol fuchsin والميثيلين بلو كانتا من ضمن الـ basic stain
+
+305
+00:21:37,910 --> 00:21:41,870
+لكن النيجروزين كانت من ضمن الـ acid stain، يبقى فيها
+
+306
+00:21:41,870 --> 00:21:46,330
+لأن في خطأ في السلايد، من نصف نيجروزين، لأنها تعتبر
+
+307
+00:21:46,330 --> 00:21:50,430
+acid stain، ما نستخدمها في الـ simple stain، الـ simple
+
+308
+00:21:50,430 --> 00:21:58,150
+stain فقط نستخدم basic stain، بعد ما نغمر السلايد
+
+309
+00:21:58,150 --> 00:22:03,550
+بالـ stain، وتنتهي المدة المحددة حسب كل صبغة، نضيف أو
+
+310
+00:22:03,550 --> 00:22:07,450
+نعمل washing، نقول، تمسك السلايد بشكل موازٍ
+
+311
+00:22:07,450 --> 00:22:13,550
+للمجرى المائي، بعدها تحاول أنك أنت تنشف الـ
+
+312
+00:22:13,550 --> 00:22:18,210
+سلايد تمامًا، air-drying، نشوف السلايد بعد ما تنشف
+
+313
+00:22:18,210 --> 00:22:21,930
+على الـ microscope، على عدسة الـ air و الـ oil lens
+
+314
+00:22:21,930 --> 00:22:28,230
+على عدسة 4x أو 10x، أو ممكن نبدأ في 10x
+
+315
+00:22:28,230 --> 00:22:35,990
+،40x وبعدين ننتقل لـ 100x، هذه الخطوات نفس الشيء
+
+316
+00:22:35,990 --> 00:22:39,590
+قلنا، smear preparation، air drying، heat fixation،
+
+317
+00:22:39,590 --> 00:22:42,970
+وبعدين، أريد أن أصبع وأشوفها تحت الميكروسكوب
+
+318
+00:22:48,490 --> 00:22:53,730
+هنا بعض النتائج لـ smear، لو جبت سؤال
+
+319
+00:22:53,730 --> 00:22:58,470
+شريحة وأقول لكم، هذه نتيجة الـ smear، نريد
+
+320
+00:22:58,470 --> 00:23:03,050
+أن ننتبه، أن نرى الشكل والـ arrangement، لأنه يكون على
+
+321
+00:23:03,050 --> 00:23:07,270
+الشكل علامة، الـ arrangement علامة، سنرى الشكل،
+
+322
+00:23:07,270 --> 00:23:11,930
+الشكل هو، إذا لاحظتم، كروي، cocci، والـ arrangement
+
+323
+00:23:11,930 --> 00:23:16,090
+في عندي خليتين ملتصقتين، يبقى diplococci، من
+
+324
+00:23:16,090 --> 00:23:18,450
+الأمثلة على الـ diplococci سنتعرف عليها، أو
+
+325
+00:23:18,450 --> 00:23:24,010
+ستأخذوها، إن النيسيريا هي تعتبر diplococci، البكتيريا
+
+326
+00:23:24,010 --> 00:23:28,750
+هنا، طبعًا، إذا لاحظتم، إنها عصوية، لكن الـ lie
+
+327
+00:23:28,750 --> 00:23:31,790
+بالنسبة للـ arrangement، no arrangement، ما في
+
+328
+00:23:31,790 --> 00:23:38,000
+عندي أي شكل، هي single، لكن الـ lie، هذه البكتيريا هي
+
+329
+00:23:38,000 --> 00:23:41,960
+شكل كروي، وبالنسبة للـ arrangement، إذا لاحظتم، على
+
+330
+00:23:41,960 --> 00:23:47,000
+شكل سلسلة، يبقى streptococci، في عندي البكتيريا
+
+331
+00:23:47,000 --> 00:23:50,700
+Acinetobacter، التي هي البكتيريا الخيطية، هذه
+
+332
+00:23:50,700 --> 00:23:56,340
+البكتيريا هي طبعًا شكلها خيطي، من خلال مشهد التشريح
+
+333
+00:23:56,340 --> 00:24:00,800
+أنا أقدر أشوفها، فهكذا نكون قد أنهينا معمل الـ smear
+
+334
+00:24:00,800 --> 00:24:07,120
+stain، سيكون مرفقًا مع الشرح فيديو عن الجزء
+
+335
+00:24:07,120 --> 00:24:12,160
+العملي، كيف الخطوات الـ smear stain مصورة وواضحة
+
+336
+00:24:12,160 --> 00:24:16,100
+وإن شاء الله تكون كل المعلومات في هذا الفصل
+
+337
+00:24:16,100 --> 00:24:19,060
+واضحة، والسلام عليكم ورحمة الله وبركاته
diff --git a/PL9fwy3NUQKwZWgAb_V1ATlRRsH-6i3cPC/DBNaI7FWU-U_raw.srt b/PL9fwy3NUQKwZWgAb_V1ATlRRsH-6i3cPC/DBNaI7FWU-U_raw.srt
new file mode 100644
index 0000000000000000000000000000000000000000..e333e0a783f8a18667c40206d774802eec25cb6b
--- /dev/null
+++ b/PL9fwy3NUQKwZWgAb_V1ATlRRsH-6i3cPC/DBNaI7FWU-U_raw.srt
@@ -0,0 +1,1352 @@
+1
+00:00:00,470 --> 00:00:04,270
+morphology of bacteria the basis for studying is
+
+2
+00:00:04,270 --> 00:00:08,070
+to study the morphology and structure of bacteria
+
+3
+00:00:08,070 --> 00:00:11,990
+قلنا هدفي من الصباغة أني أنا أتعرف على شكل
+
+4
+00:00:11,990 --> 00:00:16,170
+البكتيريا وقلنا أشكال البكتيريا ممكن تكون
+
+5
+00:00:16,170 --> 00:00:21,550
+spherical كراوية كوكس or كوكاي ممكن تكون rod shape
+
+6
+00:00:21,550 --> 00:00:26,090
+اللي هي عصوية basilas or basilae يبقى أشكال
+
+7
+00:00:26,090 --> 00:00:30,470
+البكتيريا ممكن تكون كوكاي وممكن تكون basilaeقلنا
+
+8
+00:00:30,470 --> 00:00:33,570
+على الـ Simple Stain هدفي اني انا اتعرف على الشاب
+
+9
+00:00:33,570 --> 00:00:37,650
+و الـ arrangement للبكتيريا اتعرفنا على الشاب of
+
+10
+00:00:37,650 --> 00:00:40,690
+بكتيريا طب شو بالنسبة لالـ arrangement؟ الـ
+
+11
+00:00:40,690 --> 00:00:45,210
+arrangement هو ترتيب الخلايا البكتيرية بيعتمد الـ
+
+12
+00:00:45,210 --> 00:00:48,730
+arrangement على الـ plan of division على مستوى
+
+13
+00:00:48,730 --> 00:00:52,990
+الانقسام seen in the organism bacteria may also
+
+14
+00:00:52,990 --> 00:00:58,660
+have a specific arrangement of the cellالمستوى
+
+15
+00:00:58,660 --> 00:01:04,420
+الانقسام عشان نفهم اكتر لو انقسمت الخلية البكتيرية
+
+16
+00:01:04,420 --> 00:01:08,440
+وكان مستوى الانقسام اذا ملاحظين كان vertical بشكل
+
+17
+00:01:08,440 --> 00:01:14,420
+عمودي وعططن خليتين هدول الخليتين دلهم ملتسقين مع
+
+18
+00:01:14,420 --> 00:01:19,120
+بعض ما انفصلوا وكان الشاب بالاصل ككاي يبقى بسمي ال
+
+19
+00:01:19,120 --> 00:01:24,460
+arrangement دبلو بما ان الشكل ككاي يبقى دبلو ككاي
+
+20
+00:01:24,460 --> 00:01:29,940
+لو كان الشكل في الأصل بصلاييبقى بسمي الاخر شئ
+
+21
+00:01:29,940 --> 00:01:34,200
+البكتيريا diplo بصلاي diplo الarrangement و بصلاي
+
+22
+00:01:34,200 --> 00:01:40,380
+الشكل طيب لو كان مستوى الانقسام بشكل عمودي لكن كان
+
+23
+00:01:40,380 --> 00:01:45,700
+continuous ضال مستمر مش فقط خليتين كان كوّن chain
+
+24
+00:01:45,700 --> 00:01:50,360
+of كُكّال cell كوّن سلسلة من الخلايا البكتيرية و
+
+25
+00:01:50,360 --> 00:01:54,740
+كانت الشكل في الأصل كُكّا يبقى بسمي الarrangement
+
+26
+00:01:54,740 --> 00:01:59,160
+streptoوالشياب ككاي يبقى بالنهاية اسمها
+
+27
+00:01:59,160 --> 00:02:03,700
+streptococci طب لو كان الشكل في الأصل بسلاي وكانت
+
+28
+00:02:03,700 --> 00:02:08,580
+انقسمت بشكل عمودي لكن ضالوا continually بسميها
+
+29
+00:02:08,580 --> 00:02:13,380
+streptobacillary بالنسبة لل plan of division لو
+
+30
+00:02:13,380 --> 00:02:17,320
+كان عندي مستوى الانقسام vertical و horizontal كان
+
+31
+00:02:17,320 --> 00:02:23,470
+في عندي طول و عرض بسمي الشكل tetradلو كان عندى
+
+32
+00:02:23,470 --> 00:02:28,190
+مستوى الانقسام تلاتة طول و عرض و ارتفاع بسمى الـ
+
+33
+00:02:28,190 --> 00:02:32,270
+Arrangement Sarcina احنا بنعرف او قولنا في معمل
+
+34
+00:02:32,270 --> 00:02:37,450
+الـ Microscope الصورة اللى بيعطينى ياها الـ Light
+
+35
+00:02:37,450 --> 00:02:41,490
+Microscope هي Virtual صورة افتراضية بتعطينى فقط
+
+36
+00:02:41,490 --> 00:02:45,690
+طول و عرض فالـ Sarcina انا فيه عندى مستوى الانقسام
+
+37
+00:02:45,690 --> 00:02:49,790
+كان طول و عرض و ارتفاعيبقى ما بقدر أتعرف على
+
+38
+00:02:49,790 --> 00:02:53,810
+الساركينة في الـ Light Microscope بحتاج إلى المشهر
+
+39
+00:02:53,810 --> 00:02:59,590
+التشريحي حتى أني أنا أشوف الساركينة لو انقسمت بشكل
+
+40
+00:02:59,590 --> 00:03:04,510
+Randomly، بشكل عشوائي، بسميها Staphylococcus ولو
+
+41
+00:03:04,510 --> 00:03:08,970
+كان الشكل كوكاي، بسميها Staphylococcus بشكل ممقوضي
+
+42
+00:03:08,970 --> 00:03:12,770
+لعينة، بنسميها ممقوضي ممكن يكون ما عندي
+
+43
+00:03:12,770 --> 00:03:15,770
+Arrangement تظلها Single سواء Single كوكاي أو
+
+44
+00:03:15,770 --> 00:03:19,660
+Single بصل Lightبنحكي non-arrangement يبقى هذا
+
+45
+00:03:19,660 --> 00:03:23,280
+بالنسبة للـarrangement of bacteria نرجع على ال
+
+46
+00:03:23,280 --> 00:03:27,160
+slide Diplococci are formed when the plane of
+
+47
+00:03:27,160 --> 00:03:30,280
+division is vertical بقولنا بتتكون إذا كان مستوى
+
+48
+00:03:30,280 --> 00:03:34,420
+الانقسام عمودي والنتيجة كان عندي two coccal cells
+
+49
+00:03:34,420 --> 00:03:38,980
+خليتين كرويتين لم ينفصلوا don't completely
+
+50
+00:03:38,980 --> 00:03:43,000
+separate from each other ما انفصلوا عن بعضبقولنا
+
+51
+00:03:43,000 --> 00:03:45,640
+if the cell divide in the vertical plane
+
+52
+00:03:45,640 --> 00:03:49,660
+continually and the cell don't separate لو كان
+
+53
+00:03:49,660 --> 00:03:54,140
+مستوى الانقسام عمودي لكن بشكل مستمر وهي الخلايا ما
+
+54
+00:03:54,140 --> 00:03:58,020
+انفصلت وكونت chain of cocktail cell بسمي أنا
+
+55
+00:03:58,020 --> 00:04:02,820
+الخلايا streptococci أو streptobacilli حسب الشكل
+
+56
+00:04:02,820 --> 00:04:06,740
+other arrangement لو انقسمت بشكل ربعي بسميها
+
+57
+00:04:06,740 --> 00:04:10,870
+tetrad كان مستوى الانقسام vertical و horizontalلو
+
+58
+00:04:10,870 --> 00:04:14,910
+كان المستوى الانقسام طوله عرض وارتفاع بشكل ثمانيات
+
+59
+00:04:14,910 --> 00:04:18,010
+بسميه ساركينة وقولنا الساركينة ما بشوفها في ال
+
+60
+00:04:18,010 --> 00:04:21,990
+light microscope أخر الشيء لو كان بشكل randomly أو
+
+61
+00:04:21,990 --> 00:04:26,410
+cluster بسمي ال arrangement استفيله كُكّة يبقى هذا
+
+62
+00:04:26,410 --> 00:04:31,750
+بالنسبة ليه ال arrangement ال simple stand بتعرف
+
+63
+00:04:31,750 --> 00:04:34,890
+من خلال علشاب و ال arrangement الشاب ممكن يكون
+
+64
+00:04:34,890 --> 00:04:38,110
+كُكّة يبص اللهي ال arrangement حسب مستوى الانقسام
+
+65
+00:04:38,110 --> 00:04:42,960
+إذا كان vertical دبلوVertical Continually Strictly
+
+66
+00:04:42,960 --> 00:04:46,220
+Randomly بشكل عشوائي بتكون Stiff ممكن يكون No
+
+67
+00:04:46,220 --> 00:04:50,480
+Arrangement ممكن يكون Other زي الـ Tetrad والسركي
+
+68
+00:04:50,480 --> 00:04:56,320
+هذا بالنسبة للشاب والـArrangement خلصنا من قلنا أو
+
+69
+00:04:56,320 --> 00:05:00,060
+اتكلمنا على الهدف أو الـPrincipal من الـSimple
+
+70
+00:05:00,060 --> 00:05:03,400
+Plan الـObjective هدف الـSimple Plan هلأ نيجي
+
+71
+00:05:03,400 --> 00:05:08,290
+لخطوات العمل أشهر الـProcedureاللي انا بشتغلها او
+
+72
+00:05:08,290 --> 00:05:12,350
+الخطوات حتى اشتغل الـ Stain Symbol Staining
+
+73
+00:05:12,350 --> 00:05:16,350
+Protocol in Microbiology Lab أول خطوة اللي هي الـ
+
+74
+00:05:16,350 --> 00:05:20,990
+Bacterial Sphere Preparation بدي أحضر البكتيريا
+
+75
+00:05:20,990 --> 00:05:25,490
+بقولنا تحضير البكتيريا بيعتمد على النوع الوسط اللي
+
+76
+00:05:25,490 --> 00:05:29,850
+نمت عليه البكتيريا ممكن تنمو على ميديا وسط هذا
+
+77
+00:05:29,850 --> 00:05:34,670
+الوسط ممكن يكون Agar، Agar يعني صلبةوقتها انا بصب
+
+78
+00:05:34,670 --> 00:05:39,950
+ال media في بلات في أطباق بطري في بلات وممكن تكون
+
+79
+00:05:39,950 --> 00:05:44,230
+سائل liquid او بنسميها broth broth يعني liquid
+
+80
+00:05:44,230 --> 00:05:49,390
+سائل ووقتها بصبها في tube طريقة تتحضير البكتيريا
+
+81
+00:05:49,390 --> 00:05:53,790
+بتختلف حسب إذا كان الوسط أجار أو كان broth لو كان
+
+82
+00:05:53,790 --> 00:05:58,490
+أجار طبعا أكيد هصبه في بلات بجيب ال slide و بضيف
+
+83
+00:05:58,490 --> 00:06:01,950
+drop من ال normal saline ال normal saline محلول
+
+84
+00:06:01,950 --> 00:06:08,790
+ملحيتركيزه 85% من 100% زائد Loop Full of Bacteria
+
+85
+00:06:08,790 --> 00:06:14,210
+باخد مسحة من الكلوني من الطبق بستيرايل Loop تروري
+
+86
+00:06:14,210 --> 00:06:17,990
+أنا أستخدم استيرايل Loop تكون Aseptic Technique
+
+87
+00:06:17,990 --> 00:06:22,550
+أمنع ال Contamination لو كان نيكروم Loop عندي بتم
+
+88
+00:06:22,550 --> 00:06:27,570
+تعقيمه باستخدام اللهبكاتبيه لانا كاتبة من أوسان
+
+89
+00:06:27,570 --> 00:06:31,370
+اللى هو يفضل اني انا اخد isolated colony ليش
+
+90
+00:06:31,370 --> 00:06:35,270
+isolated colony؟ عشان اضمن ان كل البكتيريا أصلها
+
+91
+00:06:35,270 --> 00:06:39,870
+واحد بالتالي هيكون شكلها واحد بعمل بعد ما أخدت
+
+92
+00:06:39,870 --> 00:06:42,870
+اللوب full of bacteria بعمل mix مع ال normal
+
+93
+00:06:42,870 --> 00:06:48,130
+saline و بروح بفرد باللوب و بحاول اني انا افردها
+
+94
+00:06:48,130 --> 00:06:53,790
+على كل slide تمام؟ هيك انا خلصت من اول طريقة اللى
+
+95
+00:06:53,790 --> 00:06:58,920
+هي الاسمية preparationطيب انا قولت بدي استخدم او
+
+96
+00:06:58,920 --> 00:07:02,680
+اني انا لازم اخد isolated colony ايش هي ال
+
+97
+00:07:02,680 --> 00:07:05,960
+isolated colony؟ انا في عندي اكتر من طريقة لزراعة
+
+98
+00:07:05,960 --> 00:07:10,060
+هنتعرف عليها في معمل ال media preparation ضمن طرق
+
+99
+00:07:10,060 --> 00:07:13,800
+الزراعة طريقة ال quaterner طريقة الأربع هاي
+
+100
+00:07:13,800 --> 00:07:17,740
+الطريقة الهدف منها احصل على isolated colony انا
+
+101
+00:07:17,740 --> 00:07:21,400
+بزرع الربع الأول تمام بجيب البكتيريا اللى انا بدي
+
+102
+00:07:21,400 --> 00:07:28,070
+ازراحهاباستخدام اللوب وبزرع في الربع الأول بعدها
+
+103
+00:07:28,070 --> 00:07:31,070
+بستخدم اللوب تاني إذا كنت بستخدم ال plastic loop
+
+104
+00:07:31,070 --> 00:07:34,970
+بستخدم اللوب الجديد وإذا بستخدم نيكروم لوب بروح
+
+105
+00:07:34,970 --> 00:07:39,930
+بعقم هذا اللوب ما برجع باخد بكتيريا تانية فقط باخد
+
+106
+00:07:39,930 --> 00:07:46,490
+من وين باخد من هذا الربع و بطلع بخطين هيك أنا في
+
+107
+00:07:46,490 --> 00:07:51,530
+هذه الطريقة بكون خففةبعدها بستخدم لو بيشزير اذا
+
+108
+00:07:51,530 --> 00:07:55,410
+plastic وبزرع الربع التالت وهكذا في الرابع الرابع
+
+109
+00:07:55,410 --> 00:07:59,550
+بهذه الطريقة بخفف ال colony بحاول اعزل ال colony
+
+110
+00:07:59,550 --> 00:08:02,790
+عن بعض و احصل على isolated colony في هن دي انا
+
+111
+00:08:02,790 --> 00:08:06,930
+صورة هذه الصورة اذا ملاحظين الربع الاول ثق و
+
+112
+00:08:06,930 --> 00:08:10,350
+المستعمرات فوق بعض الربع التاني اخف من الربع الاول
+
+113
+00:08:10,350 --> 00:08:14,560
+بدأت شوية المستعمرات تتبعد عن بعضالربع التالت ايضا
+
+114
+00:08:14,560 --> 00:08:18,040
+اخف من التاني في الرابع من اللاحظ انه ال colony
+
+115
+00:08:18,040 --> 00:08:21,740
+معزولة عن بعض فباخد انا هاي colony هيك بضمن ان
+
+116
+00:08:21,740 --> 00:08:26,220
+اصلها خلية بكتيريا واحدة يعني كل شكل الخلايا هيكون
+
+117
+00:08:26,220 --> 00:08:30,120
+نفس الشيء طيب ايش بالنسبة ل ال normal saline
+
+118
+00:08:30,120 --> 00:08:33,500
+اتكلمنا عن البكتيريا نيجي نحكي على ال normal
+
+119
+00:08:33,500 --> 00:08:37,680
+saline ال normal saline لازم يكون تركيزه 85% من
+
+120
+00:08:37,680 --> 00:08:43,500
+100% كيف بدأ احضر هذا التركيزأو ايش معنى 85 من 100
+
+121
+00:08:43,500 --> 00:08:48,900
+%؟ معنى انه 85 من 100 جرام من الـ Normal Saline هو
+
+122
+00:08:48,900 --> 00:08:53,700
+بيكون Salt زي الملح مدوبة في 100 مل من الـ
+
+123
+00:08:53,700 --> 00:08:57,720
+Distilled Water طيب لو أنا بدي كمية أكتر بروح بدرب
+
+124
+00:08:57,720 --> 00:09:02,700
+درب تدباد و ليه؟ على سبيل المثال بدي 1000 مل باخد
+
+125
+00:09:02,700 --> 00:09:07,100
+8.5 جرام من الـ Normal Saline و بدوبها ب1000 مل من
+
+126
+00:09:07,100 --> 00:09:12,290
+الـ Distilled Waterننتبه هنا إنه أنا هذا ال normal
+
+127
+00:09:12,290 --> 00:09:15,490
+saline صلب فانا استخدمت هذه الطريقة لكن لما كنا
+
+128
+00:09:15,490 --> 00:09:22,510
+نحضر كحول 70% بحط 70 مل كحول و بكمل على المية
+
+129
+00:09:22,510 --> 00:09:26,450
+distal water يعني بالنهاية بيطلع ال distal water
+
+130
+00:09:26,450 --> 00:09:29,910
+تلاتين لكن هنا أنا حطيت المية كلها distal water
+
+131
+00:09:29,910 --> 00:09:34,910
+لأنه ال normal saline هنا صلبطيب، شو وظيفة الـ
+
+132
+00:09:34,910 --> 00:09:37,450
+Normal Saline؟ احنا كاتبينها إن Normal Saline
+
+133
+00:09:37,450 --> 00:09:41,030
+بحافظ لي على الضغط الأسموازي لأنه لو كان الـ
+
+134
+00:09:41,030 --> 00:09:45,550
+Normal Saline أكتر من 85% من 100% هيصير في عندي
+
+135
+00:09:45,550 --> 00:09:49,950
+Shrinking للخلايا لو كان أقل من 85% من 100% هيصير
+
+136
+00:09:49,950 --> 00:09:55,210
+في عندي Swallowing للخلاياسبب تاني إن أنا استخدمت
+
+137
+00:09:55,210 --> 00:09:58,270
+الـ Normal Saline في هذه الطريقة لأن الكولوني
+
+138
+00:09:58,270 --> 00:10:02,510
+بتكون جافة فانا بدي إشي سائل أني أحاول أوزعها على
+
+139
+00:10:02,510 --> 00:10:05,690
+الـ Slime يبقى عشان يحفظلي على الـ Osmotic
+
+140
+00:10:05,690 --> 00:10:09,730
+Pressure وسبب تاني لأن الكولوني جافة فبدي إشي
+
+141
+00:10:09,730 --> 00:10:14,510
+أحاول يسهل عليّ توزيع الخلايا البكتيرية على الـ
+
+142
+00:10:14,510 --> 00:10:18,590
+Slime هيك خلصنا من طريقة الأجارة طيب لو كان الوسط
+
+143
+00:10:18,590 --> 00:10:22,780
+الـ Broth لو كان الوسط سائلبقول نهار مش محتاجة أنا
+
+144
+00:10:22,780 --> 00:10:27,500
+normal saline لأن هو سائل فقط بضيف drop من ال
+
+145
+00:10:27,500 --> 00:10:30,980
+bacterial broth أو ال bacterial suspension نفس
+
+146
+00:10:30,980 --> 00:10:33,780
+المعنى ال bacterial broth نفس المعنى ال bacterial
+
+147
+00:10:33,780 --> 00:10:38,180
+suspension bacterial broth معناها ميديا سائلة
+
+148
+00:10:38,180 --> 00:10:42,220
+مزروعة فيها بكتيريا bacterial suspension اللي هو
+
+149
+00:10:42,220 --> 00:10:47,100
+سائل ممكن يكون normal saline ممكن يكون ميديا سائلة
+
+150
+00:10:47,100 --> 00:10:51,310
+معلقة فيه بكتيرياوبفرد باستخدام اللوب أو في الاصل
+
+151
+00:10:51,310 --> 00:10:54,410
+ممكن ما اخد ما اخد في الادروبر اخد ال bacterial
+
+152
+00:10:54,410 --> 00:10:58,290
+suspension باستخدام اللوب احاول افرد حوالين لسلون
+
+153
+00:10:58,290 --> 00:11:02,930
+هان بهذه الطريقة ما بحط cover slip ننتبه في طريقة
+
+154
+00:11:02,930 --> 00:11:06,410
+ال wet mouth هديك ال living understand ال organism
+
+155
+00:11:06,410 --> 00:11:09,670
+بنستخدم ال wet mouth بنستخدم ال cover slip هان ما
+
+156
+00:11:09,670 --> 00:11:14,290
+بنحط cover slip هيك خلصنا من أول خطوة اللي هي ال
+
+157
+00:11:14,290 --> 00:11:18,630
+bacterial اسمه preparationلكن في بعض الأحيان ممكن
+
+158
+00:11:18,630 --> 00:11:23,190
+يصير في عندي مشاكل في السمير ممكن السمير يكون عندى
+
+159
+00:11:23,190 --> 00:11:27,210
+thick سمير وممكن يكون thin سمير طب هي مشكلة؟ اه
+
+160
+00:11:27,210 --> 00:11:32,790
+مشكلة أكيد thick سمير لو كان انا استخدمت او عملت
+
+161
+00:11:32,790 --> 00:11:36,270
+thick سمير يعني هتكون الخلايا أو الخلايا البكتيرية
+
+162
+00:11:36,270 --> 00:11:39,950
+فوق بعض فبالتالي مش هتعرفي اتميزي الشاب و ال
+
+163
+00:11:39,950 --> 00:11:44,860
+arrangement عشان نحل هذه المشكلةمنضيف ومنخفف،
+
+164
+00:11:44,860 --> 00:11:48,620
+مايعني منخفف؟ يعني منضيف another drop of normal
+
+165
+00:11:48,620 --> 00:11:54,220
+saline تنسمير يعني الخلايا بتكون قليلة في هذا ال
+
+166
+00:11:54,220 --> 00:11:57,860
+slide عشان أني أنا أحل هذه المشكلة، أش بعمل؟ take
+
+167
+00:11:57,860 --> 00:12:01,500
+another loop full of bacteria، بضيف مسحة أخرى من
+
+168
+00:12:01,500 --> 00:12:04,340
+البكتيريا، يبقى خلصنا أول خطوة اللي هي ال
+
+169
+00:12:04,340 --> 00:12:08,560
+bacterial smear preparation الخطوة التانية ال air
+
+170
+00:12:08,560 --> 00:12:13,350
+dryingبنخلّيها تنشف الـ slide في الهواء أو ممكن
+
+171
+00:12:13,350 --> 00:12:17,450
+نحطها في الـ incubator 37 درجة سيليزيا بيجففها
+
+172
+00:12:17,450 --> 00:12:22,190
+بشكل أسرع طب ليش أنا بدي أعمل هذه الخطوة؟ بقولنا
+
+173
+00:12:22,190 --> 00:12:26,910
+to prevent lysis حتى أني أمنع تحلل الخلايا طب عشان
+
+174
+00:12:26,910 --> 00:12:30,490
+نفهم أكتر ليش أنا بدي أمنع أو كيف بدي أمنع تحلل
+
+175
+00:12:30,490 --> 00:12:34,630
+الخلايا إذا أنا إذا أنا اتبعت أو عملت air drying
+
+176
+00:12:34,630 --> 00:12:38,710
+لنحكي عن الخطوة التالتة اللي هي الـ heat fixation
+
+177
+00:12:39,310 --> 00:12:44,270
+الـ heat fixation في هذه الخطوة بمرر الـ slide على
+
+178
+00:12:44,270 --> 00:12:49,250
+اللهب بسرعة تلت مرات يبقى هتتعرض ال slide أو
+
+179
+00:12:49,250 --> 00:12:53,750
+البكتيريا لحرارة في هذه الخطوة لو أنا ماعملت خطوة
+
+180
+00:12:53,750 --> 00:12:57,350
+الـ air drying و روحت على خطوة الـ heat fixation
+
+181
+00:12:57,350 --> 00:13:00,290
+هيك بيكون فيه على ال slide فيه ميه لأنه انا مانا
+
+182
+00:13:00,290 --> 00:13:05,770
+جففتها ميه زاد حرارة هيصير عندي license للخلايا
+
+183
+00:13:05,770 --> 00:13:10,250
+هتتشوه شكل البكتيرياعشان هيك لازم نجففها عشان
+
+184
+00:13:10,250 --> 00:13:14,510
+مايصير اي lysis و نحافظ على شكلها يبقى عرفنا ليش
+
+185
+00:13:14,510 --> 00:13:18,950
+خطوة خطوة ال air drying to prevent lysis هلأ نرجع
+
+186
+00:13:18,950 --> 00:13:21,950
+على ال heat fixation قولنا هاي الخطوة كيف نعملها
+
+187
+00:13:21,950 --> 00:13:27,290
+نمرر ال slide تلت مرات على اللهب بسرعة طيب ليش انا
+
+188
+00:13:27,290 --> 00:13:30,930
+بدي اعمل هاي الخطوة من اسمها fixation to fix
+
+189
+00:13:30,930 --> 00:13:34,390
+بكتيريا بدي اثبت البكتيريا على ال slide لإن انا في
+
+190
+00:13:34,390 --> 00:13:38,730
+عندي خطوات لل standing بدي أصبع بدي أغسلبدي اغسل
+
+191
+00:13:38,730 --> 00:13:41,550
+اعمل washing فبالتالي ممكن البكتيريا خلال ال
+
+192
+00:13:41,550 --> 00:13:46,590
+washing انها تروح مع الميه عشان اضمن ان البكتيريا
+
+193
+00:13:46,590 --> 00:13:50,670
+ملتصقة على ال slide بعمل خطوة ال heat fixation طب
+
+194
+00:13:50,670 --> 00:13:54,170
+كيف البكتيريا بتلتصق على ال slide؟ بقولنا ال heat
+
+195
+00:13:54,170 --> 00:13:57,730
+الحرارة بتعمل partial melting of cell wall and
+
+196
+00:13:57,730 --> 00:14:03,030
+membrane of bacteria بتعمل تذويب بشكل بسيط لـالـ
+
+197
+00:14:03,030 --> 00:14:05,990
+cell wall والـ cell membrane هذا بيساعد إنه
+
+198
+00:14:05,990 --> 00:14:11,210
+البكتيريا تلتصق على الـ slide طيب، الهدف التالت من
+
+199
+00:14:11,210 --> 00:14:14,930
+هذه الخطوة تـ kill بكتيريا إذا متذكرين في الـ
+
+200
+00:14:14,930 --> 00:14:18,690
+bacterial morphology فيه دي طريقتين قلنا، الطريقة
+
+201
+00:14:18,690 --> 00:14:21,450
+الأولى living unstained الطريقة التانية killed
+
+202
+00:14:21,450 --> 00:14:25,110
+unstained organism في هذه الطريقة قلنا killed
+
+203
+00:14:25,110 --> 00:14:29,850
+بتكون البكتيريا ميت لأنه في خطوة من خطوات الصباغة
+
+204
+00:14:29,850 --> 00:14:35,110
+متمرر الـ slide على اللهبفبالتالي البكتيريا بتموت
+
+205
+00:14:35,110 --> 00:14:39,390
+في هذه الخطوة بيصير كل البكتيريا في هذه الخطوة
+
+206
+00:14:39,390 --> 00:14:43,270
+ماشي المقصود بالـ kill، القتل؟ مقصود إنه أنا بدي
+
+207
+00:14:43,270 --> 00:14:47,370
+أوقف حركة البكتيريا بدي أوقف الـ metabolism عشان
+
+208
+00:14:47,370 --> 00:14:52,190
+أقدر أحدد الـ morphology خلصنا من خطوة الـ heat
+
+209
+00:14:52,190 --> 00:14:57,050
+fixation هذا كله إنه فيه ملاحظة إنه أنا ما أعمل
+
+210
+00:14:57,050 --> 00:15:01,900
+overheat يعني بسرعة تلت مرات بسرعةلو خلّيتها كتير
+
+211
+00:15:01,900 --> 00:15:05,600
+على اللهب هذا هيسبب destroy لالبكتيريا انا مابدى
+
+212
+00:15:05,600 --> 00:15:10,460
+تتحلل البكتيريا مابدى تتدمر لأن انا فقط بدي احافظ
+
+213
+00:15:10,460 --> 00:15:15,760
+على شكلها حتى اني انا اقدر احدد ال morphology رابع
+
+214
+00:15:15,760 --> 00:15:20,640
+خطوة لستان تختلف حسب نوع الستان سواء كانت simple
+
+215
+00:15:20,640 --> 00:15:24,360
+سواء كانت differential سواء كانت special يبقى اول
+
+216
+00:15:24,360 --> 00:15:27,380
+خطوة و تاني خطوة و تالت خطوة هي نفسها هتكون بال
+
+217
+00:15:27,380 --> 00:15:31,660
+differential و ال specialلكن الاختلاف هيكون في رقم
+
+218
+00:15:31,660 --> 00:15:35,940
+أربعة في الـ Simple Stain اللي هو ما عملنا في هذا
+
+219
+00:15:35,940 --> 00:15:41,720
+اللقاء أول خطوة باستخدم Basic Stain وعرفنا ليش
+
+220
+00:15:41,720 --> 00:15:45,700
+لأنه الـ Basic Stain بتحمل شحنة موجبة والبكتيريا
+
+221
+00:15:45,700 --> 00:15:49,320
+شحنتها الكولية ثالثة واحنا قلنا الـ Simple Stain
+
+222
+00:15:49,320 --> 00:15:53,720
+اسمها Simple بسيطة بستخدم فقط الـ agent واحد اللي
+
+223
+00:15:53,720 --> 00:15:56,520
+هو الـ Basic Stain سواء استخدمت مثليني بلوك
+
+224
+00:15:56,520 --> 00:16:01,420
+كاربولفوكسين سفرانينأي basic stain بستخدم لمدة
+
+225
+00:16:01,420 --> 00:16:04,420
+معينة بعد ما اتخلص هذه المدة بعمل washing بال
+
+226
+00:16:04,420 --> 00:16:08,460
+distil water أو ال tap water بعدها في إيش إضافي
+
+227
+00:16:08,460 --> 00:16:11,340
+قولناه في الجدول ممكن أضيف ال mordant المثبت
+
+228
+00:16:11,340 --> 00:16:15,480
+الأيضائي بعدها بغسل نفس الشيء بعمل washing هذه
+
+229
+00:16:15,480 --> 00:16:18,040
+بالنسبة لخطوات ال symbol stain
+
+230
+00:16:22,100 --> 00:16:26,400
+هنمر على الـ «سلايدات» بشكل سريع لأن تقريبًا الـ
+
+231
+00:16:26,400 --> 00:16:31,200
+«سلايدتان» الأقبل لخّصوا كل الموضوع هنمعرف للـ
+
+232
+00:16:31,200 --> 00:16:34,700
+«سمير» «سمير» is a distribution of bacterial cell
+
+233
+00:16:34,700 --> 00:16:39,140
+on a slide for the purpose of viewing them under
+
+234
+00:16:39,140 --> 00:16:42,720
+the microscope يبقى هنفي عندي تعريف مهم جدًا اللي
+
+235
+00:16:42,720 --> 00:16:46,900
+هو «سمير» هو توزيع الخلايا البكتيرية على الـ
+
+236
+00:16:46,900 --> 00:16:50,340
+«سلايد» بحيث أني أنا أقدر أشوفها تحت الميكروسكوب
+
+237
+00:16:51,220 --> 00:16:54,660
+الطريقة أول شيء أسيبتيكالي إيش يعني أسيبتيكالي؟
+
+238
+00:16:54,660 --> 00:16:58,420
+أسيبتيك تكنيك هي طرق كل الطرق لمنع ال
+
+239
+00:16:58,420 --> 00:17:02,120
+contamination من أني أستخدم Sterile Loops أستخدم
+
+240
+00:17:02,120 --> 00:17:04,600
+Sterile Normal Saline الـ Normal Saline اللي أنا
+
+241
+00:17:04,600 --> 00:17:07,060
+بدي أستخدمه لازم يكون معقّم من عقمه في الـ
+
+242
+00:17:07,060 --> 00:17:10,740
+Autoclave قبل هذا كله لازم أني أنا أعقم الbench
+
+243
+00:17:10,740 --> 00:17:14,180
+اللي هشتغل عليه كذلك الشغل لازم يكون في منطقة
+
+244
+00:17:14,180 --> 00:17:17,420
+معقمة ممكن أني أنا أشتغل جانب اللهب أو بالـSeptic
+
+245
+00:17:17,420 --> 00:17:21,470
+Cabinet كل هذه اسمها أسيبتيك تكنيكaseptically a
+
+246
+00:17:21,470 --> 00:17:24,670
+small sample of the culture is spread over a slide
+
+247
+00:17:24,670 --> 00:17:26,890
+surface هذه الخطوة هي الـ smearing preparation
+
+248
+00:17:26,890 --> 00:17:32,690
+بوزع العينة على الـ slide بعدها بعرضها لـ بعمل air
+
+249
+00:17:32,690 --> 00:17:36,370
+drying حتى أمنع الـ lysis تالت خطوة اللي هي الـ
+
+250
+00:17:36,370 --> 00:17:41,190
+fixation وهذا كل السبب to help the cell adhere to
+
+251
+00:17:41,190 --> 00:17:45,910
+the slide surface أخر خطوة اللي هي الستانك يبقى
+
+252
+00:17:45,910 --> 00:17:48,230
+هاي كل الخطوات اللي ذكرناها
+
+253
+00:17:56,130 --> 00:18:01,230
+هن في بعض الخطوات مرسومة إنه استخدم اللي هو الـ
+
+254
+00:18:01,230 --> 00:18:04,930
+Broth Media أخد Bacterial Suspension أو Bacterial
+
+255
+00:18:04,930 --> 00:18:09,230
+Broth باستخدام اللوب حاول إنه يوزّي high الـ
+
+256
+00:18:09,230 --> 00:18:15,350
+Bacterial Suspension على الـ slide ويفرطهم هن drop
+
+257
+00:18:15,350 --> 00:18:18,870
+of normal saline أخد بكتيريا لوب full of bacterial
+
+258
+00:18:18,870 --> 00:18:22,650
+حاول يعمل mix و بعدها صار في عندي بعد الـ air
+
+259
+00:18:22,650 --> 00:18:26,940
+drying هيك بنحسهاالـ Normal Saline إذا ملاحظين هو
+
+260
+00:18:26,940 --> 00:18:31,820
+شفاف Transparent لما أنا أحط الكولوني بيصير شكله
+
+261
+00:18:31,820 --> 00:18:36,620
+زي Milky appearance زي شكل الحليب إذا متذكرين احنا
+
+262
+00:18:36,620 --> 00:18:39,580
+قلنا على الـ Thick سمير و Thin سمير و كيف أنا بدي
+
+263
+00:18:39,580 --> 00:18:44,040
+أحل هذه المشكلة بسمع حكي أنا كيف بدي أعرف إن والله
+
+264
+00:18:44,040 --> 00:18:47,640
+هل هو السمير Thick ولا Thin من خلال اللون خلال
+
+265
+00:18:47,640 --> 00:18:50,760
+شغلي إذا أنا لحظة اللون كتير غامق معناه إنه ممكن
+
+266
+00:18:50,760 --> 00:18:54,150
+احتمال يكون السمير عندي Thickلو كان فاتح اللون
+
+267
+00:18:54,150 --> 00:18:57,790
+معناه احتمال يكون عندي thin smear فبحاول ان انا
+
+268
+00:18:57,790 --> 00:19:01,670
+احل المشكلة لو انا بعد ما صبغته و يصار في عندي
+
+269
+00:19:01,670 --> 00:19:05,470
+drying ممكن ان انا اعيد الشغل مرة تانية و ان انا
+
+270
+00:19:05,470 --> 00:19:09,390
+احاول ان اضيف كمية اكتر من البكتيريا او من ال
+
+271
+00:19:09,390 --> 00:19:12,250
+normal saline حسب اذا كان thick او thin smear
+
+272
+00:19:15,970 --> 00:19:20,550
+in this exercise we will use simple stand to show
+
+273
+00:19:20,550 --> 00:19:24,930
+the general structure of some bacteria بقوللي ال
+
+274
+00:19:24,930 --> 00:19:27,470
+simple stand اللي اش انا بستخدمها اني انا اتعرف
+
+275
+00:19:27,470 --> 00:19:31,870
+على شكل البكتيريا usually a single basic stand
+
+276
+00:19:31,870 --> 00:19:35,410
+قولنا بنستخدم فقط reagent واحد من ال basic stand
+
+277
+00:19:35,410 --> 00:19:40,610
+في هذه الخطوةSimple Stain لا يقدر يقدر يقدر يقدر
+
+278
+00:19:40,610 --> 00:19:42,530
+يقدر يقدر يقدر يقدر يقدر يقدر يقدر يقدر يقدر يقدر
+
+279
+00:19:42,530 --> 00:19:44,250
+يقدر يقدر يقدر يقدر يقدر يقدر يقدر يقدر يقدر يقدر
+
+280
+00:19:44,250 --> 00:19:50,610
+يقدر يقدر يقدر يقدر يقدر يقدر يقدر يقدر يقدر يقدر
+
+281
+00:19:50,610 --> 00:19:53,350
+يقدر يقدر يقدر يقدر يقدر يقدر يقدر يقدر يقدر يقدر
+
+282
+00:19:53,350 --> 00:19:53,410
+يقدر يقدر يقدر يقدر يقدر يقدر يقدر يقدر يقدر يقدر
+
+283
+00:19:53,410 --> 00:19:53,410
+يقدر يقدر يقدر يقدر يقدر يقدر يقدر يقدر يقدر يقدر
+
+284
+00:19:53,410 --> 00:19:56,650
+يقدر يقدر يقدر يقدر يقدر يقدر يقدر يقدر يقدر
+
+285
+00:20:07,320 --> 00:20:12,840
+They simply make the bacterial easier to see فقط
+
+286
+00:20:12,840 --> 00:20:18,660
+هي بتجعل البكتيريا سهل اني انا اشوفها هذه الخطوات
+
+287
+00:20:18,660 --> 00:20:24,000
+عند كل ان هو استخدم من البروث ميديا using
+
+288
+00:20:24,000 --> 00:20:28,520
+eniculating loop استخدم ال loop وأكيد طبعا لازم
+
+289
+00:20:28,520 --> 00:20:33,080
+ضروري يكون use sterile techniqueSmear the bacteria
+
+290
+00:20:33,080 --> 00:20:36,660
+بعمل بفرد البكتيريا من الـ Center حوالين و بعدين
+
+291
+00:20:36,660 --> 00:20:40,180
+بحاول أني أنا أروح على الأطراف if the bacterial
+
+292
+00:20:40,180 --> 00:20:43,480
+suspension is very thick واتكلمنا عن هذا الموضوع
+
+293
+00:20:43,480 --> 00:20:47,060
+إذا كان thick بضيف added a drop of water water
+
+294
+00:20:47,060 --> 00:20:50,540
+قصده فيها الـ normal saline تاني خطوة اللي هي الـ
+
+295
+00:20:50,540 --> 00:20:55,660
+air-drying كتب للسبب to prevent lysisالتالت خطوة
+
+296
+00:20:55,660 --> 00:21:00,560
+الـ Heat Fixation عن طريق تمريرها تمرير الـ slide
+
+297
+00:21:00,560 --> 00:21:04,620
+على اللها بتلت مرات بسرعة هذا بيعمل partial
+
+298
+00:21:04,620 --> 00:21:07,440
+melting of the cell wall and membrane of bacteria
+
+299
+00:21:07,440 --> 00:21:12,220
+and make them stick to the slide وتكلمنا عن هذا
+
+300
+00:21:12,220 --> 00:21:15,840
+الموضوع don't overheat لأنه بيسبب ليه destroy the
+
+301
+00:21:15,840 --> 00:21:22,290
+bacteriaأخر شئ بدي أعمل خطوة لستان الصبغة بدي أغمر
+
+302
+00:21:22,290 --> 00:21:27,150
+الاسمير بـ high الستان ببازكستان حسب المدة اللى
+
+303
+00:21:27,150 --> 00:21:31,110
+مكتوبة لكل نوع من أنواع الصبغات Carbolfoxin,
+
+304
+00:21:31,210 --> 00:21:34,570
+Methylenablo, نيجروزين إذا ملاحظين أنه الـ
+
+305
+00:21:34,570 --> 00:21:37,910
+Carbofoxin والميتلينيبلاو كانت من ضمن البازكستان
+
+306
+00:21:37,910 --> 00:21:41,870
+لكن النيجروزين كانت من ضمن الأسدكستان يبقى فيها
+
+307
+00:21:41,870 --> 00:21:46,330
+لأن في خطأ في ال slide من نصف نيجروزين لأنها تعتبر
+
+308
+00:21:46,330 --> 00:21:50,430
+أسدكستانما بستخدمها في الـ Simple Stain الـ Simple
+
+309
+00:21:50,430 --> 00:21:58,150
+Stain فقط بنستخدم Basic Stain بعد ما نغمر ال slide
+
+310
+00:21:58,150 --> 00:22:03,550
+بالستان وتنتهي المدة المحددة حسب كل صبغة بنضيف أو
+
+311
+00:22:03,550 --> 00:22:07,450
+بنعمل Washing بقولنا بتمسك ال slide بشكل موازي
+
+312
+00:22:07,450 --> 00:22:13,550
+يكون لالمجرى الميّه بعدها بتحاول انك انت تتنشفي ال
+
+313
+00:22:13,550 --> 00:22:18,210
+slide تمام Air-dryingبنشوف ال slide بعد ما تنشف
+
+314
+00:22:18,210 --> 00:22:21,930
+على ال microscope على عدسة ال air و ال oil lens
+
+315
+00:22:21,930 --> 00:22:28,230
+على عدسة 40 او او عشرة او ممكن نبدأ في عشرة اكس
+
+316
+00:22:28,230 --> 00:22:35,990
+اربعين وبعدين ننتقل لمية اكس هذه الخطوات نفس الشيء
+
+317
+00:22:35,990 --> 00:22:39,590
+قلنا لسمير ب preparation air drying heat fixation
+
+318
+00:22:39,590 --> 00:22:42,970
+وبعدين بدي انا اصبغ و اشوفها تحت ال microscope
+
+319
+00:22:48,490 --> 00:22:53,730
+هن بعض النتائج للـ Symbolist 10 أنا لو جبت سؤال
+
+320
+00:22:53,730 --> 00:22:58,470
+شريحة ويقولنا حد دي نتيجة الـ Symbolist 10 بدنا
+
+321
+00:22:58,470 --> 00:23:03,050
+ننتبه أنه بدنا شاب و Arrangement لأنه بيكون على
+
+322
+00:23:03,050 --> 00:23:07,270
+الشاب علاموي الـ Arrangement علامة هنشوف الشاب
+
+323
+00:23:07,270 --> 00:23:11,930
+الشاب هو إذا ملاحظين ككاي كراويةوالـ Arrangement
+
+324
+00:23:11,930 --> 00:23:16,090
+في عندي الخليتين ملتصقتين يبقى Diplococci من
+
+325
+00:23:16,090 --> 00:23:18,450
+الأمثلة على الـ Diplococci هنتعرف عليها أو
+
+326
+00:23:18,450 --> 00:23:24,010
+هتاخدوها إن نيسيريا هي تعتبر Diplococci البكتيريا
+
+327
+00:23:24,010 --> 00:23:28,750
+«هان» طبعا إذا ملاحظي إن هي عصوية بس ال «Lie»
+
+328
+00:23:28,750 --> 00:23:31,790
+بالنسبة لأ الـ Arrangement no Arrangement مافي
+
+329
+00:23:31,790 --> 00:23:38,000
+عندي أي شكل هي single بس ال «Lie»هذه البكتيريا هي
+
+330
+00:23:38,000 --> 00:23:41,960
+شكل كوكاي وبالنسبة للـarrangement إذا ملاحظين على
+
+331
+00:23:41,960 --> 00:23:47,000
+شكل إتشان يبقى streptococci في عندي البكتيريا
+
+332
+00:23:47,000 --> 00:23:50,700
+Acinetomyces اللي هي البكتيريا الخيطية هذه
+
+333
+00:23:50,700 --> 00:23:56,340
+البكتيريا هي طبعا شكلها بصلاي من خلال مشهد التشريح
+
+334
+00:23:56,340 --> 00:24:00,800
+أنا بقدر أشوفها فهيك بنكون خلصنا معمل ال symbol
+
+335
+00:24:00,800 --> 00:24:07,120
+standفيه هيكون مرفق مع الشرح الـ Video عن الجزء
+
+336
+00:24:07,120 --> 00:24:12,160
+العملي كيف الخطوات الـ Symbolistين مصورة وواضحة
+
+337
+00:24:12,160 --> 00:24:16,100
+وإن شاء الله تكون كل المعلومات في هذا الـ Chapter
+
+338
+00:24:16,100 --> 00:24:19,060
+واضحة والسلام عليكم ورحمة الله وبركاته
+
diff --git a/PL9fwy3NUQKwZWgAb_V1ATlRRsH-6i3cPC/EA0d3_cKxQQ.srt b/PL9fwy3NUQKwZWgAb_V1ATlRRsH-6i3cPC/EA0d3_cKxQQ.srt
new file mode 100644
index 0000000000000000000000000000000000000000..2cdfe8fe2ad8ef3926e955a46c244332f9fc423c
--- /dev/null
+++ b/PL9fwy3NUQKwZWgAb_V1ATlRRsH-6i3cPC/EA0d3_cKxQQ.srt
@@ -0,0 +1,15 @@
+1
+00:00:19,980 --> 00:00:24,000
+السلام عليكم ورحمة الله وبركاته، نستكمل معكم سلسلة
+
+2
+00:00:24,000 --> 00:00:28,220
+ميكروبيولوجيا أجارية إسلامية. فيديو اليوم بعنوان
+
+3
+00:00:28,220 --> 00:00:29,260
+Acid-Fasting
+
+4
+00:02:17,140 --> 00:02:18,040
+موسيقى
diff --git a/PL9fwy3NUQKwZWgAb_V1ATlRRsH-6i3cPC/EA0d3_cKxQQ_postprocess.srt b/PL9fwy3NUQKwZWgAb_V1ATlRRsH-6i3cPC/EA0d3_cKxQQ_postprocess.srt
new file mode 100644
index 0000000000000000000000000000000000000000..ab1ebea0e6962d7a16a7d45a986c8d14ef5cf8d1
--- /dev/null
+++ b/PL9fwy3NUQKwZWgAb_V1ATlRRsH-6i3cPC/EA0d3_cKxQQ_postprocess.srt
@@ -0,0 +1,16 @@
+1
+00:00:19,980 --> 00:00:24,000
+السلام عليكم ورحمة الله وبركاته، نستجمل معكم سلسلة
+
+2
+00:00:24,000 --> 00:00:28,220
+ميكروبيولوجيا أجارية الإسلامية فيديو اليوم بعنوان
+
+3
+00:00:28,220 --> 00:00:29,260
+Acid Fasting
+
+4
+00:02:17,140 --> 00:02:18,040
+موسيقى
+
diff --git a/PL9fwy3NUQKwZWgAb_V1ATlRRsH-6i3cPC/FS07l-gRrxk.srt b/PL9fwy3NUQKwZWgAb_V1ATlRRsH-6i3cPC/FS07l-gRrxk.srt
new file mode 100644
index 0000000000000000000000000000000000000000..8d68ad64699d936493d8ffad942856b1c2aed661
--- /dev/null
+++ b/PL9fwy3NUQKwZWgAb_V1ATlRRsH-6i3cPC/FS07l-gRrxk.srt
@@ -0,0 +1,11 @@
+1
+00:00:19,620 --> 00:00:24,020
+السلام عليكم ورحمة الله وبركاته، نستكمل معكم سلسلة
+
+2
+00:00:24,020 --> 00:00:28,420
+Microbiology، فيديو اليوم بعنوان الجامعة الإسلامية
+
+3
+00:00:28,420 --> 00:00:29,780
+Indus Virus 2
diff --git a/PL9fwy3NUQKwZWgAb_V1ATlRRsH-6i3cPC/FS07l-gRrxk_raw.json b/PL9fwy3NUQKwZWgAb_V1ATlRRsH-6i3cPC/FS07l-gRrxk_raw.json
new file mode 100644
index 0000000000000000000000000000000000000000..f82e93893ce952faf676721a3821d4d076216709
--- /dev/null
+++ b/PL9fwy3NUQKwZWgAb_V1ATlRRsH-6i3cPC/FS07l-gRrxk_raw.json
@@ -0,0 +1 @@
+{"segments": [{"id": 1, "seek": 2978, "start": 19.62, "end": 29.78, "text": "السلام عليكم ورحمة الله وبركاته نستكمل معاكم سلسلة Microbiology الجامعة الإسلامية فيديو اليوم بعنوان Indus Virus 2", "tokens": [6027, 3794, 37440, 25894, 24793, 4032, 2288, 35571, 3660, 21984, 4032, 26890, 4117, 9307, 3224, 8717, 14851, 24793, 1211, 20449, 995, 24793, 8608, 1211, 3794, 37977, 5818, 20027, 1793, 25724, 10943, 27884, 33688, 3794, 37440, 10632, 8978, 16254, 2407, 45595, 20498, 45030, 1863, 2407, 7649, 2333, 301, 39790, 568], "avg_logprob": -0.18250000059604646, "compression_ratio": 1.303448275862069, "no_speech_prob": 9.119510650634766e-06, "words": [{"start": 19.62, "end": 19.96, "word": "السلام", "probability": 0.8787434895833334}, {"start": 19.96, "end": 20.44, "word": " عليكم", "probability": 0.9638671875}, {"start": 20.44, "end": 20.9, "word": " ورحمة", "probability": 0.8922119140625}, {"start": 20.9, "end": 21.14, "word": " الله", "probability": 0.97509765625}, {"start": 21.14, "end": 21.92, "word": " وبركاته", "probability": 0.98935546875}, {"start": 21.92, "end": 22.78, "word": " نستكمل", "probability": 0.9136962890625}, {"start": 22.78, "end": 23.4, "word": " معاكم", "probability": 0.8191731770833334}, {"start": 23.4, "end": 24.02, "word": " سلسلة", "probability": 0.9481201171875}, {"start": 24.02, "end": 24.96, "word": " Microbiology", "probability": 0.5895182291666666}, {"start": 24.96, "end": 25.46, "word": " الجامعة", "probability": 0.96044921875}, {"start": 25.46, "end": 26.28, "word": " الإسلامية", "probability": 0.9757080078125}, {"start": 26.28, "end": 27.02, "word": " فيديو", "probability": 0.82470703125}, {"start": 27.02, "end": 27.48, "word": " اليوم", "probability": 0.979736328125}, {"start": 27.48, "end": 28.42, "word": " بعنوان", "probability": 0.912353515625}, {"start": 28.42, "end": 29.0, "word": " Indus", "probability": 0.5330810546875}, {"start": 29.0, "end": 29.34, "word": " Virus", "probability": 0.61083984375}, {"start": 29.34, "end": 29.78, "word": " 2", "probability": 0.2227783203125}], "temperature": 1.0}], "language": "ar", "language_probability": 1.0, "duration": 167.114, "duration_after_vad": 27.014375}
\ No newline at end of file
diff --git a/PL9fwy3NUQKwZWgAb_V1ATlRRsH-6i3cPC/FS07l-gRrxk_raw.srt b/PL9fwy3NUQKwZWgAb_V1ATlRRsH-6i3cPC/FS07l-gRrxk_raw.srt
new file mode 100644
index 0000000000000000000000000000000000000000..554f56eb7404cff8174b314447e5a163f31829ec
--- /dev/null
+++ b/PL9fwy3NUQKwZWgAb_V1ATlRRsH-6i3cPC/FS07l-gRrxk_raw.srt
@@ -0,0 +1,12 @@
+1
+00:00:19,620 --> 00:00:24,020
+السلام عليكم ورحمة الله وبركاته نستكمل معاكم سلسلة
+
+2
+00:00:24,020 --> 00:00:28,420
+Microbiology الجامعة الإسلامية فيديو اليوم بعنوان
+
+3
+00:00:28,420 --> 00:00:29,780
+Indus Virus 2
+
diff --git a/PL9fwy3NUQKwZWgAb_V1ATlRRsH-6i3cPC/IM6QHSK9qWU_postprocess.srt b/PL9fwy3NUQKwZWgAb_V1ATlRRsH-6i3cPC/IM6QHSK9qWU_postprocess.srt
new file mode 100644
index 0000000000000000000000000000000000000000..fcab17de0136d4b8354eafbc7def307ca71800c8
--- /dev/null
+++ b/PL9fwy3NUQKwZWgAb_V1ATlRRsH-6i3cPC/IM6QHSK9qWU_postprocess.srt
@@ -0,0 +1,2008 @@
+1
+00:00:02,250 --> 00:00:05,570
+coagulase test هو الفحص التاني اللي هنتعرف عليه في
+
+2
+00:00:05,570 --> 00:00:09,210
+هذه المحاضرة coagulase test is one of the
+
+3
+00:00:09,210 --> 00:00:12,150
+biochemical test it is very important test in the
+
+4
+00:00:12,150 --> 00:00:15,390
+microbiology هو أحد الفحوصات الكيميائية المهمة في
+
+5
+00:00:15,390 --> 00:00:20,530
+microbiology عشان أشوف هل البكتيريا عندها أنزيم ال
+
+6
+00:00:20,530 --> 00:00:23,370
+coagulase أو لأ أو هده هيساعدني في ال
+
+7
+00:00:23,370 --> 00:00:27,790
+identification the coagulase test identify whether
+
+8
+00:00:27,790 --> 00:00:33,030
+an organism produce the exoenzyme coagulaseالفحص
+
+9
+00:00:33,030 --> 00:00:37,150
+هيعرف لي البكتيريا اللي هتفرز أنزيم الـ Coagulase
+
+10
+00:00:37,150 --> 00:00:42,170
+الـ Coagulase يعتبر Exo-enzyme لكن الكتلاز كان
+
+11
+00:00:42,170 --> 00:00:46,810
+Endo-enzyme which causes the fibrinogen of blood
+
+12
+00:00:46,810 --> 00:00:50,530
+plasma to clot واللي أشهر بيعمل أنزيم الـ
+
+13
+00:00:50,530 --> 00:00:54,830
+Coagulase بيحول الفيبرينوجين الفيبرينوجين موجود في
+
+14
+00:00:54,830 --> 00:00:59,290
+البلازمة هو بروتيين موجود في blood plasma هيحول
+
+15
+00:00:59,290 --> 00:01:05,350
+الفيبرينوجين لفيبرينالـ Fibrin هي الـ Clot تمام،
+
+16
+00:01:05,350 --> 00:01:10,730
+طيب، شو الهدف لما تتكون لـ Clot؟ الجواب موجود في
+
+17
+00:01:10,730 --> 00:01:14,390
+الجملة اللي بعدها organism that produce coagulase
+
+18
+00:01:14,390 --> 00:01:18,510
+can form a protective barrier of fibrin around
+
+19
+00:01:18,510 --> 00:01:22,810
+themselves، بقولّي ال organism اللي بتقدر إنها
+
+20
+00:01:22,810 --> 00:01:26,950
+تنتج أنزيم الـ coagulase هتحول قلنا الـ Fibrinogen
+
+21
+00:01:26,950 --> 00:01:31,420
+لـ Fibrin، لـ Clotهذه الـ clot هتكون زي protective
+
+22
+00:01:31,420 --> 00:01:38,080
+barrier هتكون زي واقي حوالين البكتيريا around
+
+23
+00:01:38,080 --> 00:01:43,020
+themselves البكتيريا بقولي هتتخبى جوا هذه ال clot
+
+24
+00:01:43,020 --> 00:01:48,020
+جوا هذه الفيبرين الجلطة بالتالي making themselves
+
+25
+00:01:48,020 --> 00:01:51,320
+highly resistant to phagocytosis other
+
+26
+00:01:51,320 --> 00:01:57,940
+immunoresponse بقولي هذا ال .. الاشيهيخلّي الـ
+
+27
+00:01:57,940 --> 00:02:01,480
+Immunosystem جهاز المناعة ما يقدر يتعجب عليها
+
+28
+00:02:01,480 --> 00:02:04,540
+بالتالي هتكون highly resistant للفاغو زيتواسيس
+
+29
+00:02:04,540 --> 00:02:09,140
+طبعا الفاغو زيتواسيس من اللي هو ال Immunoresponse
+
+30
+00:02:09,140 --> 00:02:13,200
+لأ ال Immunoresponse بشكل عام كمان and some other
+
+31
+00:02:13,200 --> 00:02:17,940
+antimicrobial agent لو مضاد حيوي بده يقضي عليها مش
+
+32
+00:02:17,940 --> 00:02:22,960
+هيلاقيها هيتكون متخبية البكتيريا جوا ال clotيبقى
+
+33
+00:02:22,960 --> 00:02:26,780
+الـ Clot هتكون زي الـ protective barrier حوالين
+
+34
+00:02:26,780 --> 00:02:30,460
+البكتيريا، هتكون زي حامي حوالين البكتيريا، بالتالي
+
+35
+00:02:30,460 --> 00:02:34,240
+جهاز المناعمة راح يقدر يتعرف عليها ولا المضادات
+
+36
+00:02:34,240 --> 00:02:39,300
+الحاوية significance
+
+37
+00:02:39,300 --> 00:02:43,840
+قلنا إنه بدي أنا أعرف كل فحص شو هو الـ significant
+
+38
+00:02:43,840 --> 00:02:48,380
+لإله، شو أهمية الفحص، بين مين ومين هيفرق هذا الفحص
+
+39
+00:02:49,950 --> 00:02:53,250
+نفرق بين أنه في هذه الـ slide احنا قلنا أهمية
+
+40
+00:02:53,250 --> 00:02:57,110
+الفحص لكن الـ slide اللي قبلها أنه كان protective
+
+41
+00:02:57,110 --> 00:03:01,110
+barrier هذا إيش الهدف من الانزيم أو شو الانزيم
+
+42
+00:03:01,110 --> 00:03:07,210
+هيعمل يبقى الـ هن فائدة الانزيم لكن هن أهمية الفحص
+
+43
+00:03:07,210 --> 00:03:11,550
+الـ coagulase test is used to differentiate the
+
+44
+00:03:11,550 --> 00:03:15,130
+potentially pathogenic species Staphylococcus
+
+45
+00:03:15,130 --> 00:03:19,440
+aureus from other ground positive coccithe usually
+
+46
+00:03:19,440 --> 00:03:24,720
+non pathogenic species احنا قلنا انه لجرام
+
+47
+00:03:24,720 --> 00:03:29,580
+positive ككاى في عندي استفيله ككاى micro ككاى
+
+48
+00:03:29,580 --> 00:03:34,160
+macro ككاى و stripto ككاى فرقنا بينهم بفحص الكتلاز
+
+49
+00:03:34,160 --> 00:03:38,460
+كانت الكتلاز negative stripto لكن الكتلاز positive
+
+50
+00:03:38,460 --> 00:03:44,610
+استفيله ككاى micro ككاى macro ككاى طيبكيف نفرق بين
+
+51
+00:03:44,610 --> 00:03:47,870
+الاستافيلو والمايكرو والمايكرو؟ في فحص سنتعرف عليه
+
+52
+00:03:47,870 --> 00:03:51,710
+في المعمل اللي جاي اللي هو الـ Oxidase الاستافيلو
+
+53
+00:03:51,710 --> 00:03:55,350
+كوكاي هي Oxidase negative لكن المايكرو والمايكرو
+
+54
+00:03:55,350 --> 00:03:58,850
+Oxidase positive فعملت هذا الفحص وطلعت Oxidase
+
+55
+00:03:58,850 --> 00:04:04,490
+negative يبقى البكتيريا الاستافيلو كوكاي طيب
+
+56
+00:04:04,490 --> 00:04:09,660
+الاستافيلو كوكاي تعتبر Genusفي عندي أنا species من
+
+57
+00:04:09,660 --> 00:04:12,700
+الاستفيلو كوكاي في عندي استفيلو كوكاس aureus في
+
+58
+00:04:12,700 --> 00:04:15,360
+عندي استفيلو كوكاس epidermidis في عندي استفيلو
+
+59
+00:04:15,360 --> 00:04:19,240
+كوكاس saprophyticas كيف بدأ أفرق بين الاستفيلو
+
+60
+00:04:19,240 --> 00:04:23,440
+كوكاس species بقولني من خلال فحص الـ Coagulase
+
+61
+00:04:23,440 --> 00:04:28,120
+الاستفيلو كوكاس aureus هي تعتبر positive Coagulase
+
+62
+00:04:28,120 --> 00:04:32,240
+positive لكن الـ non pathogenic species اللي هي
+
+63
+00:04:32,240 --> 00:04:35,220
+الاستفيلو كوكاس epidermidis و الـ saprophyticas هي
+
+64
+00:04:35,220 --> 00:04:39,290
+Coagulase negativeالـ Staphylococcus aureus
+
+65
+00:04:39,290 --> 00:04:42,310
+potentially pathogenic in human and animal هي
+
+66
+00:04:42,310 --> 00:04:44,990
+تعتبر pathogenic لكن الـ Staphylococcus
+
+67
+00:04:44,990 --> 00:04:49,150
+epidermidis والـ Sabrophyticus تعتبر over-chinstic
+
+68
+00:04:49,150 --> 00:04:53,710
+انتهازية يعني بتنتهز ضعف جهاز المنارة بسميها
+
+69
+00:04:53,710 --> 00:04:58,130
+nosocomial بتسبب الـ nosocomial infection أو ال
+
+70
+00:04:58,130 --> 00:05:01,150
+hospital acquired infection أو ال health care
+
+71
+00:05:01,150 --> 00:05:05,730
+associated infection هذه نفس المسميات يعني نفس ال
+
+72
+00:05:05,730 --> 00:05:10,300
+nosocomial infectionيبقى فحص الـ Coagulase أهميته
+
+73
+00:05:10,300 --> 00:05:14,160
+أنه بيفرق للاستفيلوكوكس Aureus اللي هي بتكون
+
+74
+00:05:14,160 --> 00:05:17,140
+Coagulase positive عن باقي ال species من
+
+75
+00:05:17,140 --> 00:05:20,220
+الاستفيلوكوكس اللي هي بتكون negative الاستفيلوكوكس
+
+76
+00:05:20,220 --> 00:05:28,020
+Epidermidis و الاستفيلوكوكس Sabrophytic منلاحظ
+
+77
+00:05:28,020 --> 00:05:32,140
+بالصورة الاستفيلوكوكس قلنا هي استفيلو عنقودية فما
+
+78
+00:05:32,140 --> 00:05:36,470
+منلاحظ أنهاعلى شكل عمقود العينبو أكيد هي الـ Gram
+
+79
+00:05:36,470 --> 00:05:39,830
+-positive ككاى Gram-positive مخد اللون الviolet
+
+80
+00:05:39,830 --> 00:05:46,050
+وككاى شكلها كراو Types of Coagulase بقول لي في
+
+81
+00:05:46,050 --> 00:05:50,650
+أنواع لـ Coagulase Coagulase enzyme بقول لي فيه
+
+82
+00:05:50,650 --> 00:05:55,150
+بيكون بشكلين ممكن يكون Bound Coagulase ممكن يكون
+
+83
+00:05:55,150 --> 00:06:00,660
+Free Coagulaseالـ Bound Coagulase also called the
+
+84
+00:06:00,660 --> 00:06:04,460
+clumping factor يبقى الـ Bound له اسم تاني اللي هو
+
+85
+00:06:04,460 --> 00:06:11,480
+ال Clumping Factor is attached to bacterial cell
+
+86
+00:06:11,480 --> 00:06:15,640
+wall and react directly with the fibrinogen in
+
+87
+00:06:15,640 --> 00:06:19,420
+plasma بقولي الـ Bound من اسمه بتفرزه الخلية
+
+88
+00:06:19,420 --> 00:06:25,200
+البكتيريةوبطلع لبره لكن بيظل مرتبط بال cell wall
+
+89
+00:06:25,200 --> 00:06:28,920
+is attached to the bacterial cell wall بيظل مرتبط
+
+90
+00:06:28,920 --> 00:06:31,940
+بال cell wall قولنا إنه إيه له اسم تاني اللي هو ال
+
+91
+00:06:31,940 --> 00:06:35,760
+clamping factor and react directly with the
+
+92
+00:06:35,760 --> 00:06:39,280
+fibrinogen in plasma بيقول ليه بيصير التفاعل بشكل
+
+93
+00:06:39,280 --> 00:06:43,560
+directly وده اللي هيفرق إيه بينه وبين الفريق
+
+94
+00:06:43,560 --> 00:06:48,070
+التفاعل بيكون direct with the fibrinogenالـ
+
+95
+00:06:48,070 --> 00:06:51,270
+Substrate اللي هيشتغل عليها الـ enzyme سواء Free
+
+96
+00:06:51,270 --> 00:06:56,350
+أو Bound هي الـ Fibrinogen اللي موجودة بتكون في
+
+97
+00:06:56,350 --> 00:07:01,410
+البلازمة نحنا هنستخدم اللي هي هنفعل ال كولونيه
+
+98
+00:07:01,410 --> 00:07:04,790
+البكتيريا مع البلازمة البلازمة الموجودة فيها الـ
+
+99
+00:07:04,790 --> 00:07:08,590
+Fibrinogen بقوللي إن التفاعل بيصير بشكل مباشر
+
+100
+00:07:08,590 --> 00:07:12,210
+هيتفاعل مع الـ Fibrinogen الـ Substrate عندي هي
+
+101
+00:07:12,210 --> 00:07:15,250
+الـ Fibrinogen هيشتغل عليها الـ Coagulase بشكل
+
+102
+00:07:15,250 --> 00:07:20,290
+مباشرهتتحول الـ Fibrinogen بواسطة انزيم الـ
+
+103
+00:07:20,290 --> 00:07:24,310
+Coagulase لـ Fibrin لـ Clot يبقى في عندي انا هياها
+
+104
+00:07:24,310 --> 00:07:28,890
+في one steps خطوة واحدة لـ Fibrinogen بواسطة انزيم
+
+105
+00:07:28,890 --> 00:07:33,330
+الـ Coagulase اتحول لـ Fibrin في خطة واحدة بالتالي
+
+106
+00:07:33,330 --> 00:07:37,330
+الـ Bound Coagulase بيكون بشكل Rapid بشكل سريع
+
+107
+00:07:37,330 --> 00:07:42,350
+فبالتالي أنا بكشف عنه بالـ Slide Method فقط يبقى
+
+108
+00:07:42,350 --> 00:07:45,250
+لو بدنا نقارن الـ Bound Coagulase اسمه Clumping
+
+109
+00:07:45,250 --> 00:07:49,880
+Factorبصير بشكل directly مباشر المعادلة
+
+110
+00:07:49,880 --> 00:07:54,700
+الفيبرونيجين بتحول لفيبرين لكلو 91 steps الإشي
+
+111
+00:07:54,700 --> 00:07:58,960
+بيكون بشكل rapid الوقت بيكون سريع فبالتالي فقط
+
+112
+00:07:58,960 --> 00:08:03,860
+بكشف عنه بالـ slide method لكن الـ free coagulase
+
+113
+00:08:03,860 --> 00:08:08,720
+is an extracellular enzyme بتفرز الخلية وبيطلع
+
+114
+00:08:08,720 --> 00:08:13,100
+خارج الخلية ما بيلزق في ال bacterial cell wall زي
+
+115
+00:08:13,100 --> 00:08:17,200
+الـ bound coagulaseفبالتالي هو يُعتبر
+
+116
+00:08:17,200 --> 00:08:21,380
+Extracellular enzyme released from the cell that
+
+117
+00:08:21,380 --> 00:08:26,200
+react with a plasma component called coagulase
+
+118
+00:08:26,200 --> 00:08:29,400
+reacting factor بقولّي التفاعل بيصير بشكل
+
+119
+00:08:29,400 --> 00:08:33,440
+indirectly بشكل غير مباشر على عكس ال bound كيف
+
+120
+00:08:33,440 --> 00:08:37,900
+بيصير بشكل غير مباشر؟ بقولّي قبل ما يصير التفاعل
+
+121
+00:08:37,900 --> 00:08:42,240
+ال free coagulase بتفاعل مع مكوّن من مكونات
+
+122
+00:08:42,240 --> 00:08:46,850
+البلازمة اسمه coagulase reacting factorهذا اختصاره
+
+123
+00:08:46,850 --> 00:08:52,030
+الـ Coagulase بتفعل مع الـ Coagulase Reacting
+
+124
+00:08:52,030 --> 00:08:57,350
+Factor وهيحول لبروثرومبين لثرومبين هيتحول
+
+125
+00:08:57,350 --> 00:09:03,170
+لبروثرومبين لثرومبين بعدها لثرومبين هو اللي هيحول
+
+126
+00:09:03,170 --> 00:09:07,710
+الفيبرينوجين لفيبرين لفيبرين لكلوت يبقى عندى انا
+
+127
+00:09:07,710 --> 00:09:14,050
+two step ال Coagulaseعندما اتفاعل مع الـ Coagulase
+
+128
+00:09:14,050 --> 00:09:18,410
+Reacting Factor حوّلي البرو ثرمبن لثرمبن بعد كده
+
+129
+00:09:18,410 --> 00:09:21,970
+الثرمبن هو اللي حوّل الفيبرينوجين لفيبريني يبقى في
+
+130
+00:09:21,970 --> 00:09:26,670
+عندي two steps هياخد وقت طويل فبالتالي الـ Free
+
+131
+00:09:26,670 --> 00:09:33,070
+Coagulase بكشف عنه بـ Lithium Method Lithium
+
+132
+00:09:33,070 --> 00:09:38,310
+Method بكشف عنه الـ Free Coagulase هذا بالنسبة لـ
+
+133
+00:09:38,520 --> 00:09:42,240
+الأنواع أو الـ form of coagulase فى عندي bound و
+
+134
+00:09:42,240 --> 00:09:46,740
+فى عندي ال free coagulase بقول ليه ال reaction
+
+135
+00:09:46,740 --> 00:09:53,020
+النتيجة نفس اللى هى ال bound coagulase لكن بتحول ل
+
+136
+00:09:53,020 --> 00:09:56,540
+-ibrothrombin بعدين بتحول ل-fibrinogen و بيصير فى
+
+137
+00:09:56,540 --> 00:09:57,540
+عندي Eclot
+
+138
+00:10:06,720 --> 00:10:10,280
+هنا في مقارنة بين ال bound coagulates و ال free
+
+139
+00:10:10,280 --> 00:10:16,100
+coagulatesالـ Bound Coagulase إيه له اسم تاني قلنا
+
+140
+00:10:16,100 --> 00:10:18,400
+لـ Clumping Factor الـ Free Coagulase
+
+141
+00:10:18,400 --> 00:10:23,460
+Extracellular Coagulase لأن قلنا بيطلع برا الخلية
+
+142
+00:10:23,460 --> 00:10:27,620
+ما بيلتسق على ال bacterial cell wall ال reaction
+
+143
+00:10:27,620 --> 00:10:31,580
+بيكون direct لأنه بتحول الفيبرينوجين لفيبرين
+
+144
+00:10:31,580 --> 00:10:35,160
+بواسطة إنزيم الـ Bound Coagulase لكن الـ Free
+
+145
+00:10:35,160 --> 00:10:40,190
+Coagulase indirect لأنه بيتفاعل معالـ coagulase
+
+146
+00:10:40,190 --> 00:10:43,730
+reacting factor لـ free coagulase بالتالي بيحوله
+
+147
+00:10:43,730 --> 00:10:46,510
+لبروثرومبين لاثرومبين بعدها لاثرومبين هو اللي
+
+148
+00:10:46,510 --> 00:10:51,510
+بيحول الفيبرينوجين لفيبرين بما انه direct خطوة
+
+149
+00:10:51,510 --> 00:10:56,050
+واحدة بالتالي هياخد وقت أقل few times لكن لـ free
+
+150
+00:10:56,050 --> 00:10:59,350
+coagulase خطوة تانية بالتالي هياخد وقت أطول tag
+
+151
+00:10:59,350 --> 00:11:04,210
+timework area بقولي المساحة limited لأنه احنا قلنا
+
+152
+00:11:04,210 --> 00:11:09,030
+هو bound coagulase فقط هيكون على سطح على ال cell
+
+153
+00:11:09,030 --> 00:11:13,390
+wall موجود فبالتالي المساحة اللي ممكن يشتغل عليها
+
+154
+00:11:13,390 --> 00:11:17,550
+limited لكن ال free coagulase broad بتكون واسعة
+
+155
+00:11:17,550 --> 00:11:23,370
+لأنه بيطلع برا الخلية فممكن يشتغل في أي مكان heat
+
+156
+00:11:23,370 --> 00:11:28,030
+tolerance ال bound coagulase heat stable بتحمل
+
+157
+00:11:28,030 --> 00:11:32,190
+درجة الحرارة لكنالـ free coagulase هي الابايل
+
+158
+00:11:32,190 --> 00:11:37,530
+بتأثر بدرجة الحرارة ال bound coagulase بكشف عنه
+
+159
+00:11:37,530 --> 00:11:41,390
+بال slide and tube method لكن ال free coagulase ال
+
+160
+00:11:41,390 --> 00:11:45,270
+tube method لأن ال free ال coagulase بياخد وقت
+
+161
+00:11:45,270 --> 00:11:50,250
+طويل فبالتالي أنا ال tube method هعملها incubation
+
+162
+00:11:50,250 --> 00:11:54,330
+أربع ساعات لكن ال slide بشكل مباشر فبكشف عن ال
+
+163
+00:11:54,330 --> 00:12:00,020
+bound بال slide and tube methodالفحص يعتبر الـ
+
+164
+00:12:00,020 --> 00:12:02,920
+bound coagulase بسمي screening لكن الـ free
+
+165
+00:12:02,920 --> 00:12:09,060
+coagulase confirmatory لأن الـ bound اللي بكشف في
+
+166
+00:12:09,060 --> 00:12:13,420
+ال slide and tube method لكن ال free في ال tube
+
+167
+00:12:13,420 --> 00:12:17,280
+method يبقى ال bound coagulase يعتبر screening لكن
+
+168
+00:12:17,280 --> 00:12:19,840
+ال free coagulase يعتبر confirmatory
+
+169
+00:12:28,350 --> 00:12:31,510
+كيف بدى انا اكشف او كيف بدى اشتغل فحص الـ
+
+170
+00:12:31,510 --> 00:12:34,290
+Coagulase قولنا في درايين دى طريقتين الـ tube
+
+171
+00:12:34,290 --> 00:12:39,390
+method هتكشفلي عن وجود ال presence of bound or
+
+172
+00:12:39,390 --> 00:12:43,250
+free لكن ال slide method فقط هتكشفلي على ال bound
+
+173
+00:12:43,250 --> 00:12:47,050
+coagulase فبالتالي ال tube method تعتبر
+
+174
+00:12:47,050 --> 00:12:50,370
+confirmatory لكن ال slide method تعتبر screening
+
+175
+00:12:50,370 --> 00:12:56,680
+test فقط بتكشفلي على ال bound coagulaseعشان هيك لو
+
+176
+00:12:56,680 --> 00:13:00,600
+عملت slide method وطلعت negative لازم أؤكد بال
+
+177
+00:13:00,600 --> 00:13:04,620
+tube method عشان أكشف عن ال free coagulase اللي ما
+
+178
+00:13:04,620 --> 00:13:13,940
+قدرت slide method تكشف ليه عنه بالنسبة
+
+179
+00:13:13,940 --> 00:13:17,460
+لل slide method هنشرح كيف slide method slide
+
+180
+00:13:17,460 --> 00:13:19,760
+method بقول ب GB slide
+
+181
+00:13:22,080 --> 00:13:46,120
+وبتعملي بتحطي drop من الـ relative plasma مع
+
+182
+00:13:46,120 --> 00:13:48,080
+touch of bacteria
+
+183
+00:14:10,280 --> 00:14:14,120
+يبقى طريقة ال slide method drop of rabbit plasma
+
+184
+00:14:14,120 --> 00:14:17,200
+مع touch of بكتيريا اللى انا بدي اكشف هل عندها ال
+
+185
+00:14:17,200 --> 00:14:21,940
+coagulase او لأ بعمل mix و بشوف النتيجة السؤال هان
+
+186
+00:14:21,940 --> 00:14:26,460
+انا ليش استخدمت plasma ما استخدمت serum السؤال
+
+187
+00:14:26,460 --> 00:14:29,820
+التانى ليش استخدمت rabbit plasma ما استخدمت human
+
+188
+00:14:29,820 --> 00:14:33,850
+plasmaأول شيء لماذا استخدمنا إبلازمة وما استخدمنا
+
+189
+00:14:33,850 --> 00:14:37,110
+سيرام الـ substrate اللي هيشتغل عليها ال enzyme هي
+
+190
+00:14:37,110 --> 00:14:40,690
+الفيبرينوجين الفيبرينوجين موجود في البلازمة مش
+
+191
+00:14:40,690 --> 00:14:44,910
+موجود في السيرام كيف أنا أحصل على إبلازمة بسحب عين
+
+192
+00:14:44,910 --> 00:14:49,470
+الدم و بحطها بتيوب فيه anticoagulant مانع للتجلط
+
+193
+00:14:49,470 --> 00:14:53,550
+عشان مايصير في عندي تجلط كلوت لأنه لو صار في عندي
+
+194
+00:14:53,550 --> 00:14:57,920
+تجلط هيتحول الفيبرينوجين لفيبرين فبالتاليهك
+
+195
+00:14:57,920 --> 00:15:01,900
+الفيبرينوجين تم استهلاكه طب أنا بديه الفيبرينوجين
+
+196
+00:15:01,900 --> 00:15:06,020
+ك substrate فبالتالي أنا بستخدم البلازما لأن
+
+197
+00:15:06,020 --> 00:15:09,880
+البلازما ما بيصير في عندى cloth ما بتتحول
+
+198
+00:15:09,880 --> 00:15:13,340
+الفيبرينوجين لفيبرين الفيبرينوجين بيضل موجود as
+
+199
+00:15:13,340 --> 00:15:17,780
+substrate لكن ال serum ال serum كيف بدي أحصل على
+
+200
+00:15:17,780 --> 00:15:21,200
+serum بس بعين الدم و بحطها في plain ال tube عادي
+
+201
+00:15:21,200 --> 00:15:26,770
+مافيها مانع للتجلط بتركها تتجلط بعدين بفصلهابيتحول
+
+202
+00:15:26,770 --> 00:15:30,330
+الفيبرينوجين لفيبرين يعني تم استهلاك الفيبرينوجين
+
+203
+00:15:30,330 --> 00:15:34,210
+مش موجود عند الفيبرينوجين في السيرام بالتالي انا
+
+204
+00:15:34,210 --> 00:15:37,950
+مابقدر اشتغل عليه لأنه انا بدي الفيبرينوجين as
+
+205
+00:15:37,950 --> 00:15:42,030
+substrate عشان يشتغل عليه ال enzyme عشان هيك لازم
+
+206
+00:15:42,030 --> 00:15:47,660
+يكون plasma مش serumبالنسبة لـ Rabbit Plasma ليش
+
+207
+00:15:47,660 --> 00:15:51,700
+أنا استخدمت Rabbit Plasma لأن لـ Human Plasma في
+
+208
+00:15:51,700 --> 00:15:55,340
+عندي Inhibitor للإنزايم للـ Coagulase Enzyme
+
+209
+00:15:55,340 --> 00:16:00,040
+بالتالي ممكن يعطيني False Negative Result يبقى
+
+210
+00:16:00,040 --> 00:16:02,860
+احنا حطينا Drop of Rabbit Plasma ال Rabbit Plasma
+
+211
+00:16:02,860 --> 00:16:06,500
+موجود في ال substrate اللي هو الفيبرينوجين
+
+212
+00:16:22,650 --> 00:16:27,030
+Touch of bacteria اللى موجود أو مش موجود حسب
+
+213
+00:16:27,030 --> 00:16:29,650
+البكتيريا اللى فيها ال enzyme اللى هو الـ
+
+214
+00:16:29,650 --> 00:16:30,290
+Coagulase
+
+215
+00:16:39,470 --> 00:16:43,890
+بعمل mix عشان يشتغل ال enzyme على ال substrate لو
+
+216
+00:16:43,890 --> 00:16:48,010
+موجود هشوف ياندي eclot هشوف agglutination زي
+
+217
+00:16:48,010 --> 00:16:51,510
+حبيبات زي اللي بنشوفها بفحص فصائل الدم يبقى هاي
+
+218
+00:16:51,510 --> 00:16:55,630
+النتيجة positive هي coagulase positive لكن لو مش
+
+219
+00:16:55,630 --> 00:17:00,670
+موجود ال enzyme مش هشوف أي حبيبات هيكون في عندي
+
+220
+00:17:00,670 --> 00:17:05,530
+smooth suspensionبقول لي place a drop of distilled
+
+221
+00:17:05,530 --> 00:17:09,150
+water or saline near the drop of plasma as control
+
+222
+00:17:09,150 --> 00:17:24,110
+نكتبها negative control بقول
+
+223
+00:17:24,110 --> 00:17:27,950
+لي أنت أول إشي بتحط drop من ال rabbit plasma و
+
+224
+00:17:27,950 --> 00:17:31,550
+بعدين بتحط ال touch of bacteria بقولنا ممكن أن نحط
+
+225
+00:17:31,800 --> 00:17:35,460
+أو لازم نحط pistol water أو normal saline جنب
+
+226
+00:17:35,460 --> 00:17:40,340
+الربط في البلازما هذا هيكون as negative control
+
+227
+00:17:40,340 --> 00:17:56,420
+كيف يعني أنا جبت ال slide قلنا
+
+228
+00:17:56,420 --> 00:17:58,600
+اضيف drop من الربط في البلازما
+
+229
+00:18:05,830 --> 00:18:12,910
+هان وهان كمان ال drop من distilled water
+
+230
+00:18:12,910 --> 00:18:18,210
+او
+
+231
+00:18:18,210 --> 00:18:19,850
+normal saline
+
+232
+00:18:28,620 --> 00:18:30,960
+الـ drop من الـ rabbit plasma والـ drop من الـ
+
+233
+00:18:30,960 --> 00:18:33,220
+distal water أو الـ normal saline بعدين بجيب
+
+234
+00:18:33,220 --> 00:18:35,900
+البكتيريا اللى انا بدي اكشف هل موجودة عندها
+
+235
+00:18:35,900 --> 00:18:43,220
+الانزيم ولا لأ و بحط هنا بكتيريا يعني
+
+236
+00:18:43,220 --> 00:18:50,860
+في كل drop هنا بكتيريا هحط وهنا
+
+237
+00:18:50,860 --> 00:18:53,200
+نفس البكتيريا اللى انا بدي اكشف عليها
+
+238
+00:19:18,370 --> 00:19:22,050
+يبقى بنحط رابط بلازما drop من الـ distal water
+
+239
+00:19:22,050 --> 00:19:25,150
+بجيب البكتيريا اللى بدى أكشف عنها هل عندها الانزيم
+
+240
+00:19:25,150 --> 00:19:28,690
+ولا لأ بحطها drop و بعمل mix مع رابط البلازما و
+
+241
+00:19:28,690 --> 00:19:31,730
+بحطها كولونى من البكتيريا اللى أنا بدى أكشف عندها
+
+242
+00:19:31,730 --> 00:19:36,090
+الانزيم نفس اللى حطيتها هنا و بعمل mix طيب ليش هذه
+
+243
+00:19:36,090 --> 00:19:38,710
+الفكرة قولنا هذا ال distal water أو ال normal
+
+244
+00:19:38,710 --> 00:19:41,470
+saline مع البكتيريا اللى بدى أكشف عنها تعتبر as
+
+245
+00:19:41,470 --> 00:19:46,870
+negative control هلأ لو البكتيريا عندها ال enzyme
+
+246
+00:19:46,870 --> 00:19:51,850
+لو البكتيرياهي عندها ال enzyme هكيد هتشتغل على ال
+
+247
+00:19:51,850 --> 00:19:54,570
+substrate اللي هي الفيبرينوجين اللي موجود في
+
+248
+00:19:54,570 --> 00:20:00,490
+البلازما وهتعطيني هتحول الفيبرينوجين لفيبرين هيصير
+
+249
+00:20:00,490 --> 00:20:03,970
+في عندي agglutination هيكون في عندي clot يبقى
+
+250
+00:20:03,970 --> 00:20:09,770
+بتكون النتيجة لو positive هي هتكون positive لو
+
+251
+00:20:09,770 --> 00:20:14,230
+عندها ال enzyme هكيد هي هتكون positive لكن لو أنا
+
+252
+00:20:14,230 --> 00:20:18,360
+عملتنفس البكتيريا اللى عندها الانزيم عملت mix مع
+
+253
+00:20:18,360 --> 00:20:22,980
+ديستلواتر أكيد هتكون النتيجة هان نيجاتف مش هيكون
+
+254
+00:20:22,980 --> 00:20:28,980
+فيه عندى أي إكلوت أي حبيبات ليش لأنه مافيه عند
+
+255
+00:20:28,980 --> 00:20:31,760
+السبسترات اللى هيشتغل عليها الانزيم هان فيه عند
+
+256
+00:20:31,760 --> 00:20:34,060
+البلازما موجود فيها الفيبرينجين لكن ال normal
+
+257
+00:20:34,060 --> 00:20:37,080
+saline أو الديستلواتر مش موجود في السبسترات اللى
+
+258
+00:20:37,080 --> 00:20:39,720
+هو الفيبرينجين اللى هيشتغل عليها الانزيم يعني حتى
+
+259
+00:20:39,720 --> 00:20:44,030
+لو كان الانزيمموجود في البكتيريا اكيد في ال
+
+260
+00:20:44,030 --> 00:20:47,450
+negative control هتكون negative ماهيكون في عندي
+
+261
+00:20:47,450 --> 00:20:51,130
+agglutination طيب هذه في الحال كانت هي positive
+
+262
+00:20:51,130 --> 00:20:55,750
+result خلينا نكتب هان وكان في عندي هان positive
+
+263
+00:20:55,750 --> 00:20:59,830
+result لو كان عندها ال enzyme
+
+264
+00:21:11,410 --> 00:21:15,190
+طيب لو كانت ما عندها البكتيريا لو جبت بكتيريا
+
+265
+00:21:15,190 --> 00:21:18,990
+تانية هذه البكتيريا هي negative لل coagulase ما
+
+266
+00:21:18,990 --> 00:21:23,470
+عندها الانزيم ال coagulase هعمل mix مع ال rabbit
+
+267
+00:21:23,470 --> 00:21:27,410
+plasma فأكيد هتكون النتيجة هان مش مش موجود الانزيم
+
+268
+00:21:27,410 --> 00:21:31,250
+لكن موجود ال substrate لكن مش هيصير في عندي تفاعل
+
+269
+00:21:31,250 --> 00:21:37,630
+هتكون negative لو حطيتها مع الالنظام الطبيعي
+
+270
+00:21:37,630 --> 00:21:41,770
+لايوجد فيه أنزيمات أو سبسترات فهو لن يكون فيه
+
+271
+00:21:41,770 --> 00:21:47,850
+اغلوتناشن فهو نيجاتيف يبقى هنا في حال كانت نيجاتيف
+
+272
+00:21:47,850 --> 00:21:48,210
+result
+
+273
+00:22:05,780 --> 00:22:09,360
+يبقى في كلتال حالتان الـ negative control لازم
+
+274
+00:22:09,360 --> 00:22:14,180
+يكون عندها نيجاتيف لش لأنه ال normal saline مش
+
+275
+00:22:14,180 --> 00:22:18,040
+موجود في عندى ال substrate اللى هي ال fibrinogen
+
+276
+00:22:18,040 --> 00:22:23,240
+اللى هيشتغل عليها ال enzyme طيب لو كانت النتيجة
+
+277
+00:22:23,240 --> 00:22:28,440
+عملت جبت بكتيريا وحطيتها مع البلازمه وطلعت
+
+278
+00:22:28,440 --> 00:22:32,960
+positive وحطيتها
+
+279
+00:22:32,960 --> 00:22:41,650
+مع ال normal saline وبرضه طلعتpositive احنا
+
+280
+00:22:41,650 --> 00:22:45,070
+قلنا انه ال negative control لازم يكون negative
+
+281
+00:22:45,070 --> 00:22:48,490
+لأنه ال normal cell line ماعندي فيبرينوجين مش
+
+282
+00:22:48,490 --> 00:22:51,350
+موجود ال substrate اللي هيشتغل عليه ال enzyme طيب
+
+283
+00:22:51,350 --> 00:22:54,550
+من وين صار عندي ال agglutination في ال negative
+
+284
+00:22:54,550 --> 00:22:58,350
+control و اللي هنا بيكون في عندي مشكلة قلنا ممنوع
+
+285
+00:22:58,350 --> 00:23:01,350
+تطلع بال negative control positive لأنه مافي
+
+286
+00:23:01,350 --> 00:23:03,990
+substrate الفيبرينوجين اللي هيشتغل عليه ال enzyme
+
+287
+00:23:04,540 --> 00:23:08,900
+طب ايش السبب اللي خلّى صار عندى فيه agglutination
+
+288
+00:23:08,900 --> 00:23:13,320
+بقول السبب ان البكتيريا بتعمل autoagglutination
+
+289
+00:23:13,320 --> 00:23:19,460
+يعني بشكل ذات بدون وجود enzyme autoagglutination
+
+290
+00:23:33,940 --> 00:23:39,480
+في عندي تفسيرها ان في عندي auto agglutination مش
+
+291
+00:23:39,480 --> 00:23:43,860
+ناتج عن وجود إنزايم الكواليولازي هي بتفرز clumping
+
+292
+00:23:43,860 --> 00:23:50,000
+factor فبيصير في عندي agglutination يبقى بالثانبال
+
+293
+00:23:50,000 --> 00:23:53,020
+انا مش عارفة في هذه العينة لو كانت في ال .. لو
+
+294
+00:23:53,020 --> 00:23:55,600
+كانت انا خلطت بـ Irreversible plasma مع البكتيريا
+
+295
+00:23:55,600 --> 00:23:59,290
+في هذه الحالةأنا مش عارفة هل البكتيريا عندها انزيم
+
+296
+00:23:59,290 --> 00:24:03,190
+الـ coagulase وعملة agglutination ولا ما عندها
+
+297
+00:24:03,190 --> 00:24:07,970
+وكان ال agglutination اللي ظهر في ال sample من ال
+
+298
+00:24:07,970 --> 00:24:13,140
+autoagglutination بقوللي الحل في هايإن أنا عشان
+
+299
+00:24:13,140 --> 00:24:15,780
+أعرف في هذه الحلقة لو طلع positive في positive
+
+300
+00:24:15,780 --> 00:24:20,060
+لازم أتأكد بالـ tube method قولنا الـ tube method
+
+301
+00:24:20,060 --> 00:24:24,440
+هي confirmatory تأكيدية لـ slides screening فلازم
+
+302
+00:24:24,440 --> 00:24:27,160
+أنا أعمل ال tube method اللي هنتعرف عليها كمان
+
+303
+00:24:27,160 --> 00:24:33,140
+شوية ال tube method هي اللي هتعرفني هل هو auto
+
+304
+00:24:33,140 --> 00:24:36,800
+agglutination ولا لأ لو طلعت positive يبقى هي مش
+
+305
+00:24:36,800 --> 00:24:40,660
+auto agglutination لو طلعت negative يبقى هوالـ
+
+306
+00:24:40,660 --> 00:24:44,460
+issue اللي صار auto agglutination والنتيجة هي
+
+307
+00:24:44,460 --> 00:24:48,800
+negative مش positive يبقى هذا بالنسبة لـ slide
+
+308
+00:24:48,800 --> 00:24:49,280
+method
+
+309
+00:25:06,200 --> 00:25:10,400
+طبعاً بنعمل بعد ما أحنا عملنا اللي هو drop of
+
+310
+00:25:10,400 --> 00:25:12,900
+rabbit plasma مع touch of بكتيريا وحطينا negative
+
+311
+00:25:12,900 --> 00:25:18,580
+control بقولي بتعملي mix بالrabbit
+
+312
+00:25:18,580 --> 00:25:22,540
+plasma أو بالكمان بالwater أو ال normal saliva
+
+313
+00:25:22,540 --> 00:25:27,180
+يعني اللي هو negative control بعدين بشوف النتيجة
+
+314
+00:25:27,180 --> 00:25:31,540
+بقولي
+
+315
+00:25:31,540 --> 00:25:35,470
+observefor clumping in the coagulase plasma and
+
+316
+00:25:35,470 --> 00:25:39,430
+homogenous suspension in the control at 20 to 30
+
+317
+00:25:39,430 --> 00:25:43,350
+seconds بقوللي بلاحظ ال clumping اللي موجود فيه ال
+
+318
+00:25:43,350 --> 00:25:46,690
+coagulase plasma ال drop اللي حطيتها من البلازما و
+
+319
+00:25:46,690 --> 00:25:50,230
+ال drop اللي حطيته في ال control هنا في عندي
+
+320
+00:25:50,230 --> 00:25:53,750
+ملاحظات clump that will not mix uniformly into
+
+321
+00:25:53,750 --> 00:25:57,510
+coagulase plasma indicate a positive test بقوللي
+
+322
+00:25:57,970 --> 00:26:01,290
+الـ Positive result بيقول في عيني بيكون Clumping
+
+323
+00:26:01,290 --> 00:26:06,230
+تجمع من اللي فيه بيكون عيني حبيبات Clots أجلوتناشن
+
+324
+00:26:06,230 --> 00:26:09,990
+زي فصائل الدم لكن بالنسبة للـ Negative result
+
+325
+00:26:09,990 --> 00:26:12,770
+بيكون في عيني Uniform suspension أو Smooth
+
+326
+00:26:12,770 --> 00:26:16,230
+suspension بيكون ال suspension كتير ناعم مافي أي
+
+327
+00:26:16,230 --> 00:26:20,250
+Clot تمام هذه النتيجة بتكون Negative result يبقى
+
+328
+00:26:20,250 --> 00:26:23,090
+الـ Positive result بيكون عيني Clumping تجمع لكن
+
+329
+00:26:23,090 --> 00:26:27,390
+الـ Negative Uniform suspension Clumping in both
+
+330
+00:26:27,390 --> 00:26:30,750
+testsindicate that the organism autoagglutination
+
+331
+00:26:30,750 --> 00:26:34,670
+at is at is unsuitable for the slide coagulast
+
+332
+00:26:34,670 --> 00:26:40,250
+test قلنا لو أنا عملت الفحص و طلعت في ال sample و
+
+333
+00:26:40,250 --> 00:26:43,470
+في ال control كان عندي positive positive هذا بدل
+
+334
+00:26:43,470 --> 00:26:46,870
+عن انه عندي في autoagglutination ال
+
+335
+00:26:46,870 --> 00:26:50,250
+autoagglutination مش مناسب لل slide coagulast test
+
+336
+00:26:50,250 --> 00:26:53,850
+لازم أأكد الفحص بال tube method لأنه هي ال
+
+337
+00:26:53,850 --> 00:26:58,000
+confirmatorywhen the autoagglutination is observed
+
+338
+00:26:58,000 --> 00:27:00,280
+واللي بقولنا الـ tube method قلنا لازم أني أنا
+
+339
+00:27:00,280 --> 00:27:04,520
+أشتغلها بدل من ال slide method لأن قلنا ال tube
+
+340
+00:27:04,520 --> 00:27:07,180
+method هو ال confirmatory
+
+341
+00:27:16,790 --> 00:27:19,790
+procedure of a tube method خلصنا من ال slide
+
+342
+00:27:19,790 --> 00:27:22,470
+method وعرفنا كيف بنشتغلها وقولنا هي screening
+
+343
+00:27:22,470 --> 00:27:26,890
+method هلأ هنتكلم على ال confirmatory method اللي
+
+344
+00:27:26,890 --> 00:27:31,450
+هو ال tube method اللي بيكشف عن ال free and bound
+
+345
+00:27:31,450 --> 00:27:35,210
+عشان هي أنا قولت يعتبر confirmatory لأنه بيكشف عن
+
+346
+00:27:35,210 --> 00:27:40,230
+ال freeوالـ Bound ما بيكشف عن في حال كان فيني Auto
+
+347
+00:27:40,230 --> 00:27:44,870
+-agglutination برضه الـ tube method أنا بستخدمها
+
+348
+00:27:44,870 --> 00:27:47,330
+بدل من ال slide method فبالتالي هو يعتبر
+
+349
+00:27:47,330 --> 00:27:51,970
+confirmatory لكن ال slide فقط بتكشفلي عن ال bound
+
+350
+00:27:51,970 --> 00:27:57,560
+ال bound coagulaseأي فحص كيميائي أول جملة بقول
+
+351
+00:27:57,560 --> 00:28:00,680
+using a culture that is less than 24 hours old
+
+352
+00:28:00,680 --> 00:28:04,000
+بقولنا أي فحص كيميائي لازم ال culture تكون fresh
+
+353
+00:28:04,000 --> 00:28:09,580
+لأن ال enzyme activity بقل و بقول بتاخد من مزرعة
+
+354
+00:28:09,580 --> 00:28:14,470
+أو من culture a garden inhibitory agariplateNon
+
+355
+00:28:14,470 --> 00:28:19,030
+-inhibitory يعني أنه أخد الكولوني من أجار non
+
+356
+00:28:19,030 --> 00:28:22,670
+-selective media أفضل أني أنا أشتغل من general
+
+357
+00:28:22,670 --> 00:28:25,970
+media لأن الـ selective media فيها inhibitor هذه
+
+358
+00:28:25,970 --> 00:28:30,830
+الـinhibitor ممكن تأثر على الإنزايم أشهر الطريقة
+
+359
+00:28:30,830 --> 00:28:34,910
+بقولنا أنه بتجيب 2 ل4 كولونيز من البكتيريا اللي
+
+360
+00:28:34,910 --> 00:28:40,720
+بدك تكشف عندها الإنزايم موجود أو لأوبتجيبيهم هاي و
+
+361
+00:28:40,720 --> 00:28:44,520
+بتحطيهم في one ml of rabbit plasma وبتعملي لهم
+
+362
+00:28:44,520 --> 00:28:49,800
+incubation لمدة أربع ساعات على درجة حرارة سبعة و
+
+363
+00:28:49,800 --> 00:28:55,420
+تلاتين خلال الأربع ساعات كل شوية بطلع و بحرك بلاحظ
+
+364
+00:28:55,420 --> 00:28:58,360
+إذا اتكوّن اتجلطة أو لأ أكيف اتكوّن اتجلطة فيه
+
+365
+00:28:58,360 --> 00:29:04,320
+بيكون كلوت زي حبيبات في السائل أو ممكن نلاحظ إن
+
+366
+00:29:04,320 --> 00:29:08,160
+السائل بدأ يجمد فبالتالي هاي النتيجة ال positive
+
+367
+00:29:08,870 --> 00:29:31,830
+يبقى الـ tube method الـ
+
+368
+00:29:31,830 --> 00:29:34,330
+tube method بجيب اتنين
+
+369
+00:29:59,820 --> 00:30:10,280
+of bacteria زائد 1
+
+370
+00:30:10,280 --> 00:30:13,640
+ml of rabbit plasma
+
+371
+00:30:28,250 --> 00:30:44,630
+بعملهم incubation على
+
+372
+00:30:44,630 --> 00:30:51,770
+درجة حرارة 37 درجة سليزيا لمدة 4 hour
+
+373
+00:31:01,560 --> 00:31:06,300
+في الـ Incubator 37 درجة سليسيس لمدة 4 ساعات وبشوف
+
+374
+00:31:06,300 --> 00:31:11,500
+النتيجة قولنا خلال 4 ساعات بحاول أني انا أشيك على
+
+375
+00:31:11,500 --> 00:31:15,840
+ال tube بشوف اذا في عندي اتكون إقلوت ولا لأ بشوف
+
+376
+00:31:15,840 --> 00:31:20,000
+إذا السائل جمد أو لأ هلأ لو كانت النتيجة positive
+
+377
+00:31:20,000 --> 00:31:27,220
+كان في عندي إقلوت
+
+378
+00:31:31,890 --> 00:31:36,870
+أو لاحظت ان السائل جمد فتمام يبقى بحكي انه هي
+
+379
+00:31:36,870 --> 00:31:41,190
+النتيجة positive في عندي انزيم الـ coagulase لكن
+
+380
+00:31:41,190 --> 00:31:51,210
+لو ماكان في عندي اي clot وكانت نوف ال clot السائل
+
+381
+00:31:51,210 --> 00:31:55,310
+دلوا زي ما هو بقولي النتيجة ما بسجلها negative
+
+382
+00:31:55,310 --> 00:32:00,830
+تمام ما منسجل النتيجة negative بقولناباروح انا
+
+383
+00:32:00,830 --> 00:32:06,070
+بعمل incubation بكمل ال incubation ل 24 ساعة احنا
+
+384
+00:32:06,070 --> 00:32:09,390
+عملنا 4 ساعات يبقى ضال في عندي 20 ساعة بعمل
+
+385
+00:32:09,390 --> 00:32:13,570
+incubation لمدة 20 ساعة لكن على ال room
+
+386
+00:32:13,570 --> 00:32:17,210
+temperature يبقى لو ما كان عندى تكون ال cloth
+
+387
+00:32:17,210 --> 00:32:21,030
+مابحكي negative بعملها incubation هاي النتيجة يبقى
+
+388
+00:32:21,030 --> 00:32:24,390
+بعملها incubation
+
+389
+00:32:34,180 --> 00:32:40,660
+at room temperature لمدة
+
+390
+00:32:40,660 --> 00:32:46,120
+20 ساعة و
+
+391
+00:32:46,120 --> 00:32:50,940
+اللي هو of total 24 hours احنا عملنا 4 ساعات يبقى
+
+392
+00:32:50,940 --> 00:32:55,240
+بدي عشان اكمل لل 24 بحطها 24 ساعة لكن على room
+
+393
+00:32:55,240 --> 00:32:59,680
+temperature بعد بعد ما تخلص ال 20 ساعة يعني ال
+
+394
+00:32:59,680 --> 00:33:04,980
+total 24 ساعة لوكان في عندي no clot يبقى خلاص
+
+395
+00:33:04,980 --> 00:33:08,800
+بعتمد النتيجة negative لكن لو اتغير وصار في عندي
+
+396
+00:33:08,800 --> 00:33:12,980
+clot يبقى هي positive يبقى هنا في المرحلة هاي انا
+
+397
+00:33:12,980 --> 00:33:17,840
+بحكي انه لو اتكون clot يبقى هي positive
+
+398
+00:33:26,710 --> 00:33:33,330
+لكن هان خلاص بعد 24 ساعة بتكون كافية انه انا احكي
+
+399
+00:33:33,330 --> 00:33:40,410
+انه هي negative نو اكلوت او السائل ما جمد يبقى
+
+400
+00:33:40,410 --> 00:33:45,030
+باعتبر انه نتيجة هان negative يبقى بعد 24 ساعة
+
+401
+00:33:45,030 --> 00:33:49,370
+بأكد النتيجة ال negative هذا بالنسبة لليتيوب
+
+402
+00:33:49,370 --> 00:33:52,890
+method هيك الطريقة هيك الشغل لليتيوب method
+
+403
+00:34:00,080 --> 00:34:03,320
+بقولنا negative test بقولنا لو الفحص كان negative
+
+404
+00:34:03,320 --> 00:34:06,320
+بعد أربع ساعات بعمله على ال room temperature زي ما
+
+405
+00:34:06,320 --> 00:34:10,500
+قولنا عشان بنكمل ل total of 24 ساعة يعني بنحط
+
+406
+00:34:10,500 --> 00:34:14,820
+عشرين ساعة في ال room temperature كيف بقرأ النتيجة
+
+407
+00:34:14,820 --> 00:34:17,880
+بقولنا بتميلي let you و بتلاحظي اللي هو clotting
+
+408
+00:34:17,880 --> 00:34:18,720
+of plasma
+
+409
+00:34:28,300 --> 00:34:31,660
+بنقرأ النتائج بقولّي إنه الـ Positive test for
+
+410
+00:34:31,660 --> 00:34:34,680
+Coagulase production result بكون في عندي Clotting
+
+411
+00:34:34,680 --> 00:34:38,840
+of the rabbit plasma لو في عندي إشي بسيط من لو
+
+412
+00:34:38,840 --> 00:34:41,640
+كانت ال Clot حتى لو بسيطة أنا بعتبرها Positive
+
+413
+00:34:41,640 --> 00:34:46,860
+result بقولّي النتيجة بكتبها من Range 0 to plus 4
+
+414
+00:34:46,860 --> 00:34:51,670
+Zero معناه إنهنوع Geolase Activity يعني Zero بتكون
+
+415
+00:34:51,670 --> 00:34:56,730
+Negative البلازمة بيبقى Liquid بيضلوا البلازمة
+
+416
+00:34:56,730 --> 00:35:01,590
+سائل مافي عندى اي Clot لكن بالنسبة لـ Plus One الـ
+
+417
+00:35:01,590 --> 00:35:05,630
+Plus One انا بعتبرها اللى هي weak positive بيكون
+
+418
+00:35:05,630 --> 00:35:10,630
+فيه عندى Clot لكن خفيف السائل مش جامد كتير زي الـ
+
+419
+00:35:10,630 --> 00:35:13,310
+Positive Four بالنسبة لـ Plus Two و Plus Three
+
+420
+00:35:13,310 --> 00:35:16,610
+بنعتبرها Intermediate Positive بيكون فيه عندى Clot
+
+421
+00:35:16,930 --> 00:35:20,130
+لكن مش زي الـ plus four الـ plus four بيكون
+
+422
+00:35:20,130 --> 00:35:24,230
+البلازما صار عندى جامد بشكل كامل طب أنا مهادة
+
+423
+00:35:24,230 --> 00:35:27,410
+بعتبرها strong positive الـ plus four يبقى strong
+
+424
+00:35:27,410 --> 00:35:30,390
+positive يبقى zero تعتبر negative لكن الـ plus one
+
+425
+00:35:30,390 --> 00:35:33,650
+plus two plus three plus four تعتبر positive لكن
+
+426
+00:35:33,650 --> 00:35:38,870
+ال positive بتختلف plus four تعتبر strong positive
+
+427
+00:35:38,870 --> 00:35:42,610
+لكن plus one تعتبر weak positive احنا قولنا اي
+
+428
+00:35:42,610 --> 00:35:47,660
+درجة من لكلت انا بعتبرها positive testكل احنا قلنا
+
+429
+00:35:47,660 --> 00:35:53,540
+اي نتيجة Zero ماكان في عندي ضلوا ال plasma سائل
+
+430
+00:35:53,540 --> 00:35:56,840
+بعد 4 ساعات بحطها على ال room temperature و بكمل
+
+431
+00:35:56,840 --> 00:35:59,860
+incubation لمدة 24 ساعة
+
+432
+00:36:03,310 --> 00:36:06,230
+في انديهان ملاحظات مهمة بقول لي when the slide
+
+433
+00:36:06,230 --> 00:36:09,490
+test is employed all negative slide reaction must
+
+434
+00:36:09,490 --> 00:36:13,490
+be confirmed by the tube test قولنا لو اشتغلت ال
+
+435
+00:36:13,490 --> 00:36:18,290
+slide method وطلعت negative لازم انا اشتغل ال tube
+
+436
+00:36:18,290 --> 00:36:21,430
+method لأن احنا قولنا ال tube يعتبر confirmatory
+
+437
+00:36:21,430 --> 00:36:26,250
+test هو بيكشفلي على ال free و ال bound لأن ال
+
+438
+00:36:26,250 --> 00:36:30,090
+slide test لو انت اشتغلتيها وطلعت negative هي
+
+439
+00:36:30,090 --> 00:36:35,530
+بتكون negative لمن؟ لل boundلكن أنا بدي أكشف عن
+
+440
+00:36:35,530 --> 00:36:39,470
+الـ Free Bluetooth Method فبالتالي بستخدم
+
+441
+00:36:39,470 --> 00:36:43,090
+Bluetooth Method عشان أشوف هل عندها Free ولا لأ لو
+
+442
+00:36:43,090 --> 00:36:46,270
+طلعت Positive بـ Bluetooth Method يبقى عندها Free
+
+443
+00:36:46,270 --> 00:36:49,430
+Coagulase لو طلعت Negative بـ Bluetooth Method بعد
+
+444
+00:36:49,430 --> 00:36:52,150
+ما عملتها بالـ Slide وطلعت Negative يبقى ما عندها
+
+445
+00:36:52,150 --> 00:36:57,900
+لا Free ولا Boundالتكنيكية الـ slide agglutination
+
+446
+00:36:57,900 --> 00:37:04,180
+قد تؤدي إلى نتيجة فالس بوزيتف بقول لي أن الـ slide
+
+447
+00:37:04,180 --> 00:37:08,620
+method ممكن تعطيني نتيجة فالس بوزيتف لماذا؟ لأن
+
+448
+00:37:08,620 --> 00:37:12,520
+بعض
+
+449
+00:37:12,520 --> 00:37:16,580
+الأنواع من البكتيريا تنتج نتيجة فالس بوزيتف الـ
+
+450
+00:37:16,580 --> 00:37:19,460
+clumping factor نفسه هو معناه أنها بتعمل auto
+
+451
+00:37:19,460 --> 00:37:23,580
+agglutination يبقى بتعطيني نتيجة فالس بوزيتف في
+
+452
+00:37:23,580 --> 00:37:29,110
+الـ slideعشان هيك لازم انا اعيد النتيجة بالـ tube
+
+453
+00:37:29,110 --> 00:37:32,330
+method قولنا الـ tube method هو يعتبر confirmatory
+
+454
+00:37:32,330 --> 00:37:36,310
+و لازم كمان انا اعمل negative control لو طلعت معي
+
+455
+00:37:36,310 --> 00:37:39,270
+positive معناه ان في عندي لو طلع negative control
+
+456
+00:37:39,270 --> 00:37:42,750
+positive معناه في عندي autoagglutination لازم اعيد
+
+457
+00:37:42,750 --> 00:37:46,720
+الفحص بالـ tube method ال confirmatoryلو بالـ tube
+
+458
+00:37:46,720 --> 00:37:50,840
+method طلع positive يبقى هي بتكون coagulase
+
+459
+00:37:50,840 --> 00:37:54,400
+positive لكن لو طلع negative بتكون هي coagulase
+
+460
+00:37:54,400 --> 00:37:57,680
+negative ال agglutination اللي دهر هو نتيجة ال
+
+461
+00:37:57,680 --> 00:38:01,800
+autoagglutination فقط spontaneous agglutination
+
+462
+00:38:01,800 --> 00:38:04,240
+may occur when rough culture are used بقوللي
+
+463
+00:38:04,240 --> 00:38:06,640
+spontaneous agglutination هو نفس ال
+
+464
+00:38:06,640 --> 00:38:10,800
+autoagglutinationبلاحظه خصوصًا لو أخدت البكتيريا
+
+465
+00:38:10,800 --> 00:38:15,500
+من لو كانت الـ colony non-capsulated رف معناه إنه
+
+466
+00:38:15,500 --> 00:38:21,160
+non-capsulated بكتيريا ليش أنا بلاحظ من الـ auto
+
+467
+00:38:21,160 --> 00:38:24,020
+-agglutination بيصير في الـ non-capsulated بكتيريا
+
+468
+00:38:24,020 --> 00:38:29,140
+أحنا قلنا الكوسول يعتبر أو يستخدم ك protection
+
+469
+00:38:29,140 --> 00:38:33,570
+fromالـ Phagocytosis والـ Coagulase Enzyme نفس
+
+470
+00:38:33,570 --> 00:38:37,070
+الفكرة قلنا بيحول الفيبرينوجين لفيبرين الفيبرين
+
+471
+00:38:37,070 --> 00:38:40,990
+لكلوت البكتيريا بتتخبى جوا هذه الفيبرين فبالتالي
+
+472
+00:38:40,990 --> 00:38:44,750
+بتحميها من الـ Phagocytosis يبقى لو ما عندها
+
+473
+00:38:44,750 --> 00:38:47,970
+capsule أكيد هتلجأ للـ Coagulase أو لو ما عندها
+
+474
+00:38:47,970 --> 00:38:51,910
+Coagulase أكيد هتلجأ للـ Autoagglutination عشان
+
+475
+00:38:51,910 --> 00:38:55,610
+يصير في عندي عشان تحميها من الـ Phagocytosis
+
+476
+00:38:55,610 --> 00:38:59,480
+فبنلاحظ أن الـ Spontaneous Agglutinationبيكون فيه
+
+477
+00:38:59,480 --> 00:39:03,580
+الـ non-capsulated bacteria بقولي آخر إشي أنه ال
+
+478
+00:39:03,580 --> 00:39:07,140
+tube method أفضل من ال slide method قلنا لأنه ال
+
+479
+00:39:07,140 --> 00:39:10,820
+tube method بيكشف عن ال free و ال bound لكن ال
+
+480
+00:39:10,820 --> 00:39:15,280
+slide بيكشف عن ال bound فقط ال slide بيصير فيه
+
+481
+00:39:15,280 --> 00:39:18,340
+اندياط و agglutination لازم أني أنا أعمل ال tube
+
+482
+00:39:18,340 --> 00:39:23,310
+method عشان أتأكد ال slide test should be readvery
+
+483
+00:39:23,310 --> 00:39:26,050
+quickly بقولّي لو انت اشتغلت بـ slide method لازم
+
+484
+00:39:26,050 --> 00:39:29,630
+تقرأيها بسرعة false positive result ممكن انه يصير
+
+485
+00:39:29,630 --> 00:39:34,170
+ال test should then perform with organism detected
+
+486
+00:39:34,170 --> 00:39:37,590
+from high salt media such as manitol salt agar
+
+487
+00:39:37,590 --> 00:39:42,510
+احنا قلنا انه اي فحص كيميائي لازم ان انا اشتغله من
+
+488
+00:39:42,510 --> 00:39:45,390
+general media ال manitol salt agar تعتبر selective
+
+489
+00:39:45,390 --> 00:39:48,590
+media فيها inhibitor اللي هو ال salt بقول ال salt
+
+490
+00:39:48,590 --> 00:39:53,550
+ممكن انه يؤثر على الانزيمات فبالتاليتعطيني False
+
+491
+00:39:53,550 --> 00:39:56,950
+Positive Result هذه النتيجة إذا ما نلاحظ الـ
+
+492
+00:39:56,950 --> 00:39:59,030
+Staphylococcus aureus قولنا هي Positive للـ
+
+493
+00:39:59,030 --> 00:40:01,650
+Coagulase في عندى حبيبات، في عندى Clots لكن
+
+494
+00:40:01,650 --> 00:40:05,090
+Staphylococcus epidermidis هي Negative للـ
+
+495
+00:40:05,090 --> 00:40:07,850
+Coagulase منلاحظ أنه في عندى Smooth Suspension
+
+496
+00:40:07,850 --> 00:40:10,990
+Uniformة في عندى أي حبيبات هذا بالنسبة للـ Slide
+
+497
+00:40:10,990 --> 00:40:14,490
+Method لكن للـ Tube Method الـ Coagulase Positive
+
+498
+00:40:14,490 --> 00:40:18,620
+منلاحظ أن الـ Plasma جمدتلكن الـ Coagulase
+
+499
+00:40:18,620 --> 00:40:22,200
+Negative أنه مافي عندي Clot و البلازمه ضاله سائل
+
+500
+00:40:22,200 --> 00:40:26,480
+حيث أننا خلصنا الـ Coagulase Test و Catalase Test
+
+501
+00:40:26,480 --> 00:40:31,180
+و حيث أننا خلصنا الـ Chapter لليوم والسلام عليكم
+
+502
+00:40:31,180 --> 00:40:32,560
+ورحمة الله وبركاته
+
diff --git a/PL9fwy3NUQKwZWgAb_V1ATlRRsH-6i3cPC/IO73Edx7DW0.srt b/PL9fwy3NUQKwZWgAb_V1ATlRRsH-6i3cPC/IO73Edx7DW0.srt
new file mode 100644
index 0000000000000000000000000000000000000000..5a83911d348c163c930d21784f169e3edffa44b8
--- /dev/null
+++ b/PL9fwy3NUQKwZWgAb_V1ATlRRsH-6i3cPC/IO73Edx7DW0.srt
@@ -0,0 +1,702 @@
+1
+00:00:00,520 --> 00:00:03,960
+السلام عليكم ورحمة الله وبركاته، يعطيكم العافية
+
+2
+00:00:03,960 --> 00:00:06,760
+جميعًا في هذه المحاضرة، إن شاء الله، هنبدأ أول معمل
+
+3
+00:00:06,760 --> 00:00:10,840
+من مساق الـ General Microbiology Laboratory سابقًا
+
+4
+00:00:10,840 --> 00:00:15,420
+عرفنا الـ General Microbiology: it is the study of
+
+5
+00:00:15,420 --> 00:00:19,400
+microorganisms. هو العلم الذي يدرس الكائنات الدقيقة
+
+6
+00:00:19,400 --> 00:00:23,000
+الـ microorganisms التي ندرسها في علم الـ micro
+
+7
+00:00:23,000 --> 00:00:26,560
+biology: it is small living organisms that cannot be
+
+8
+00:00:26,560 --> 00:00:31,440
+seen by the naked eye. هي كائنات دقيقة حية لا ترى بالعين
+
+9
+00:00:31,440 --> 00:00:35,300
+المجردة. تكلمنا أيضًا عن أقسام علم الـ Microbiology
+
+10
+00:00:35,300 --> 00:00:38,900
+وكان من ضمنها الـ Medical Microbiology، المجال الذي
+
+11
+00:00:38,900 --> 00:00:43,560
+نركز فيه دراستنا عليه كعملين في المجال الطبي: it
+
+12
+00:00:43,560 --> 00:00:46,140
+is the study of microbes and their associated
+
+13
+00:00:46,140 --> 00:00:50,280
+with the host. هو العلم الذي يدرس الميكروبات وعلاقتها
+
+14
+00:00:50,280 --> 00:00:55,220
+بالعائل، الـ host.  الـ host الذي نقصد فيه الإنسان: هل
+
+15
+00:00:55,220 --> 00:01:00,320
+هذا الـ microbe يسبب مرضًا للـ host؟ هل الميكروب
+
+16
+00:01:00,320 --> 00:01:06,360
+harmless  للعائل، أم هل الميكروب يكون
+
+17
+00:01:06,360 --> 00:01:10,800
+مفيدًا للعائل (beneficial). كل هذا سنتعرف عليه. الهدف من
+
+18
+00:01:10,800 --> 00:01:14,660
+هذا المساق هو أن يتعرف الطلاب على مختلف
+
+19
+00:01:14,660 --> 00:01:19,920
+أشكال الحياة في عالم الميكروبات.  هدف من معمل الـ
+
+20
+00:01:19,920 --> 00:01:22,920
+microbiology أنه يكون لدي خلفية عن علم الأحياء
+
+21
+00:01:22,920 --> 00:01:26,800
+الدقيقة، نتعرف على الميكروبات أكثر في المحاضرة
+
+22
+00:01:26,800 --> 00:01:31,040
+التعريفية. حكينا أنه هدفي من معمل الـ Microbiology
+
+23
+00:01:31,040 --> 00:01:35,440
+أنه نعزل بكتيريا من عينات مرضية ونشخصها عبر فحوصات
+
+24
+00:01:35,440 --> 00:01:39,820
+مختلفة. الجملتان نفس الشيء، لأن حتى إن أنا أقدر أعزل
+
+25
+00:01:39,820 --> 00:01:43,420
+بكتيريا من عينات مرضية وأشخصها، أكيد لازم يكون
+
+26
+00:01:43,420 --> 00:01:47,480
+لدي خلفية عن علم الأحياء الدقيقة، أعرف خصائص كل
+
+27
+00:01:47,480 --> 00:01:51,850
+ميكروب، أعرف الظروف التي ينمو فيها، أعرف الأمراض
+
+28
+00:01:51,850 --> 00:01:56,710
+التي يسببها كل microbe. كل المعلومات التي تخص الـ
+
+29
+00:01:56,710 --> 00:02:00,150
+microbes. Although the study of microbiology
+
+30
+00:02:00,150 --> 00:02:04,250
+includes bacteria, viruses, algae, and protozoa, this
+
+31
+00:02:04,250 --> 00:02:07,450
+lab will concentrate primarily on bacteria.
+
+32
+00:02:07,450 --> 00:02:12,230
+حكينا في علم الـ Medical Microbiology، هو علم واسع
+
+33
+00:02:12,230 --> 00:02:17,420
+يدرس الكائنات الدقيقة: من بكتيريا، من فيروسات، من
+
+34
+00:02:17,420 --> 00:02:22,920
+فطريات، طحالب، و الـ protozoa.  الـ protozoa هي الأوليات
+
+35
+00:02:22,920 --> 00:02:28,440
+لكن قلنا في هذا المعمل، في الـ essential medical
+
+36
+00:02:28,440 --> 00:02:32,800
+microbiology، سنركز على البكتيريا. طيب، شو هي
+
+37
+00:02:32,800 --> 00:02:37,660
+الـ protozoa؟ الـ protozoa معناها بالعربي هي الأوليات
+
+38
+00:02:37,660 --> 00:02:42,220
+طيب، شو تفرق عن البكتيريا؟ نقول أن الـ Protozoa هي
+
+39
+00:02:42,220 --> 00:02:47,600
+unicellular microorganisms، كائنات وحيدة الخلية
+
+40
+00:02:47,600 --> 00:02:52,480
+eukaryotic. شو يعني eukaryotic؟ يعني حقيقية النواة
+
+41
+00:02:52,480 --> 00:02:56,500
+طيب، شو عكس الـ eukaryotic؟ عكس الـ eukaryotic الـ
+
+42
+00:02:56,500 --> 00:03:01,420
+prokaryotic. طيب، شو هي البكتيريا؟ البكتيريا هي عبارة
+
+43
+00:03:01,420 --> 00:03:06,240
+عن unicellular microorganisms، كائنات وحيدة الخلية
+
+44
+00:03:06,240 --> 00:03:11,210
+مثل الـ protozoa، لكن تختلف عن البروتوزوا بأنها
+
+45
+00:03:11,210 --> 00:03:17,370
+prokaryotic. بدايةً، يبقى البروتوزوا unicellular
+
+46
+00:03:17,370 --> 00:03:22,690
+eukaryotic microorganisms. البكتيريا unicellular
+
+47
+00:03:22,690 --> 00:03:27,310
+prokaryotic microorganisms. تشبه بأنها unicellular
+
+48
+00:03:27,310 --> 00:03:32,710
+لكن تختلف بأن البروتوزوا eukaryotic و البكتيريا
+
+49
+00:03:32,710 --> 00:03:37,940
+prokaryotic.  فلنرى شو الفرق بين الـ eukaryotic
+
+50
+00:03:37,940 --> 00:03:43,240
+و الـ prokaryotic. الـ prokaryotic طبعًا هي بدائية النواة
+
+51
+00:03:43,240 --> 00:03:48,080
+مثل البكتيريا. الـ eukaryotic هي حقيقية النواة مثل
+
+52
+00:03:48,080 --> 00:03:53,640
+البروتوزوا، مثلًا الإنسان، نحن نعتبر eukaryotic
+
+53
+00:03:53,640 --> 00:03:59,200
+لو بدنا نعمل مقارنة، فلنبدأ أول وجه مقارنة، نتكلم
+
+54
+00:03:59,200 --> 00:04:04,010
+على number of chromosomes، عدد الكروموسومات في الـ
+
+55
+00:04:04,010 --> 00:04:08,650
+eukaryotic، نحن الإنسان عدد الكروموسومات 46 كروموسوم
+
+56
+00:04:08,650 --> 00:04:13,850
+لكن الـ prokaryotic، مثل البكتيريا، تتكون من one
+
+57
+00:04:13,850 --> 00:04:19,410
+chromosome. يبقى أول وجه مقارنة بالنسبة لـ number of
+
+58
+00:04:19,410 --> 00:04:24,350
+chromosomes.  طيب، shape of chromosome، لو قارنّا شكل
+
+59
+00:04:24,350 --> 00:04:28,970
+الكروموسوم بين الـ prokaryotic و الـ eukaryotic، الـ
+
+60
+00:04:28,970 --> 00:04:33,940
+eukaryotic، الإنسان، يعتبر شكل الكروموسوم linear، لكن
+
+61
+00:04:33,940 --> 00:04:39,300
+الـ prokaryotic يُعتبر circular. هاي ثاني وجه
+
+62
+00:04:39,300 --> 00:04:43,800
+مقارنة، الـ shape of a chromosome. الـ prokaryotic
+
+63
+00:04:43,800 --> 00:04:48,620
+يُعتبر circular، لكن الـ eukaryotic يُعتبر linear.
+
+64
+00:04:48,620 --> 00:04:55,340
+لو حكينا عن الـ mutation rate، معدل حدوث الطفرات،
+
+65
+00:04:55,340 --> 00:04:59,840
+الـ prokaryotic، نقول أن معدل حدوث الطفرات فيها
+
+66
+00:04:59,840 --> 00:05:05,740
+يُعتبر high rate، لكن الـ eukaryotic تُعتبر low rate.
+
+67
+00:05:05,740 --> 00:05:09,980
+طيب، لو تكلمنا عن السبب الذي يجعل معدل حدوث
+
+68
+00:05:09,980 --> 00:05:14,900
+الطفرات في الـ eukaryotic أقل من الـ prokaryotic،
+
+69
+00:05:15,630 --> 00:05:19,410
+الـ eukaryotic، حقيقية النواة، مثل الإنسان، نحن نعرف
+
+70
+00:05:19,410 --> 00:05:23,550
+في كل مرحلة من مراحل الـ cell cycle، بيصير فيه
+
+71
+00:05:23,550 --> 00:05:26,950
+فيه آلية تصحيح لأي طفرة، لأنه فيه checkpoints،
+
+72
+00:05:26,950 --> 00:05:31,490
+وبالتالي أكيد فرصة حدوث الطفرات في الـ eukaryotic
+
+73
+00:05:31,490 --> 00:05:39,010
+أقل. هذا وجه المقارنة الثالث لـ mutation rate، طبعًا
+
+74
+00:05:39,010 --> 00:05:45,050
+من وجوه المقارنة المهمة أيضًا، لكي نفرق بين الـ
+
+75
+00:05:45,050 --> 00:05:49,410
+prokaryotic و eukaryotic، الريبوسوم. الريبوسوم هو
+
+76
+00:05:49,410 --> 00:05:54,530
+مصنع البروتين في الخلية، سواء كان الـ prokaryotic
+
+77
+00:05:54,530 --> 00:05:59,870
+أو الـ eukaryotic. الريبوسوم يتكون من قطعتين: قطعة
+
+78
+00:05:59,870 --> 00:06:04,630
+صغيرة وقطعة كبيرة. الاختلاف يكون في سرعة الترسيب
+
+79
+00:06:04,630 --> 00:06:08,850
+لكل من هاتين القطعتين، وأيضًا سرعة ترسيب الريبوسوم
+
+80
+00:06:08,850 --> 00:06:13,630
+نقيسها. في الـ prokaryotic، عندما جئنا بالـ ribosome و
+
+81
+00:06:13,630 --> 00:06:17,950
+روحنا رسّبناه في جهاز الـ centrifuge، وجدنا سرعة
+
+82
+00:06:17,950 --> 00:06:24,070
+الترسيب 70S، لكن عندما فصلنا القطعة الكبيرة وحدها و
+
+83
+00:06:24,070 --> 00:06:27,730
+القطعة الصغيرة وحدها، القطعة الكبيرة كانت سرعة
+
+84
+00:06:27,730 --> 00:06:33,830
+الترسيب 50S، والقطعة الصغيرة كانت 30S. هذا
+
+85
+00:06:33,830 --> 00:06:38,130
+بالنسبة لـ Sedimentation Rate. أما بالنسبة لـ
+
+86
+00:06:38,130 --> 00:06:42,280
+eukaryotic، عندما رسّبنا الـ ribosome بشكل كامل في
+
+87
+00:06:42,280 --> 00:06:47,680
+جهاز الـ centrifuge، وجدنا أنه يترسب بسرعة 80S.
+
+88
+00:06:47,680 --> 00:06:52,460
+عندما فصلنا القطعة الكبيرة عن القطعة الصغيرة، القطعة
+
+89
+00:06:52,460 --> 00:06:57,700
+الكبيرة كانت سرعة الترسيب 60S، أما القطعة الصغيرة
+
+90
+00:06:57,700 --> 00:07:05,410
+كانت 40S. تمام. هذه وجوه المقارنة بين الـ
+
+91
+00:07:05,410 --> 00:07:08,670
+prokaryotic و الـ eukaryotic. طبعًا فيه وجوه
+
+92
+00:07:08,670 --> 00:07:14,110
+مقارنة أخرى، لكن هذه أهم وجوه المقارنة.  قلنا في
+
+93
+00:07:14,110 --> 00:07:18,270
+sedimentation rate، أن سرعة الترسيب لـ prokaryotic
+
+94
+00:07:18,270 --> 00:07:23,650
+الريبوسوم بشكل كامل 70S. طيب، إيش هي الـ S؟ الـ S هي
+
+95
+00:07:23,650 --> 00:07:29,590
+اختصار لـ Svedberg، وهي عبارة عن وحدة قياس تقيس
+
+96
+00:07:29,590 --> 00:07:34,610
+الـ rate of sedimentation. يبقى لو نعرف الـ Svedberg
+
+97
+00:07:34,610 --> 00:07:39,350
+as the unit measures the rate of sedimentation
+
+98
+00:07:39,350 --> 00:07:44,030
+rather than size. هي وحدة تقيس معدل سرعة الترسيب
+
+99
+00:07:44,030 --> 00:07:49,270
+للبروتين، مش حجم البروتين الذي يترسب. طيب، أنا ليش
+
+100
+00:07:49,270 --> 00:07:52,670
+قلت أنه ليس حجم البروتين الذي يترسب؟ لأننا قلنا
+
+101
+00:07:52,670 --> 00:07:56,030
+عندما رسّبنا القطعة الكبيرة كانت في الـ Svedberg
+
+102
+00:07:56,030 --> 00:08:00,230
+units، كانت سرعة الترسيب 50S، عندما رسّبنا القطعة
+
+103
+00:08:00,230 --> 00:08:04,620
+الصغيرة، كانت سرعة الترسيب 30S، لكن عندما رسّبنا
+
+104
+00:08:04,620 --> 00:08:09,580
+الريبوسوم كاملًا كان 70S، ما كان مجموعهم 80S. يبقى نحن
+
+105
+00:08:09,580 --> 00:08:13,440
+لا نجمع هذه السرعة التي يترسب عندها الـ protein
+
+106
+00:08:13,440 --> 00:08:19,740
+ككل. تمام. هذا بالنسبة لوجوه المقارنة بين الـ
+
+107
+00:08:19,740 --> 00:08:22,320
+prokaryotic و الـ eukaryotic.
+
+108
+00:08:25,080 --> 00:08:29,720
+البكتيريا are unicellular prokaryotic microorganisms.
+
+109
+00:08:29,720 --> 00:08:34,560
+تكلمنا أنها عبارة عن unicellular، وحيدة الخلية،
+
+110
+00:08:34,560 --> 00:08:39,560
+prokaryotic، بدائية النواة، microorganism. طبعًا لو أنا
+
+111
+00:08:39,560 --> 00:08:43,440
+غيّرت: unicellular eukaryotic microorganism،
+
+112
+00:08:43,440 --> 00:08:49,610
+أتحدث عن protozoa that divide by binary fission.
+
+113
+00:08:49,610 --> 00:08:54,250
+تنقسم بواسطة الانشطار الثنائي، أو binary fission.
+
+114
+00:08:54,250 --> 00:09:00,210
+نفس الكلمة. نعرف أن binary fission، a process
+
+115
+00:09:00,210 --> 00:09:04,850
+by which one bacterium splits into two. أن الخلية
+
+116
+00:09:04,850 --> 00:09:11,070
+البكتيرية الواحدة تنقسم (splits) إلى خليتين
+
+117
+00:09:11,070 --> 00:09:14,730
+بكتيريتين. هاتين الخليتين البكتيريتين تكونان
+
+118
+00:09:14,730 --> 00:09:19,790
+identical، وتكونان typical للخلية الأصل. طيب،
+
+119
+00:09:19,790 --> 00:09:24,890
+بالنسبة للبكتيريا، كثير من الناس عندما يسمعون كلمة بكتيريا
+
+120
+00:09:24,890 --> 00:09:29,310
+أول ما يخطر ببالهم أنها شيء ضار، لكن الحقيقة ليست
+
+121
+00:09:29,310 --> 00:09:34,430
+هكذا. الميكروبات بشكل عام، ممكن أن تكون pathogenic وممكن
+
+122
+00:09:34,430 --> 00:09:38,630
+أن تكون non-pathogenic. الـ non-pathogenic يعني غير
+
+123
+00:09:38,630 --> 00:09:44,550
+ممرضة. وهذا معظم الميكروبات، تصنف منها وتعتبر non-
+
+124
+00:09:44,550 --> 00:09:49,310
+pathogenic. لكن الـ pathogenic microbes، التي هي التي
+
+125
+00:09:49,310 --> 00:09:52,790
+ممرضة. ما يعني pathogenic أنها تسبب disease.
+
+126
+00:09:52,790 --> 00:09:57,570
+نسبة قليلة من الـ microbes تعتبر pathogenic. يبقى
+
+127
+00:09:57,570 --> 00:10:02,410
+النسبة الأكبر، أو معظمها، تكون non-pathogenic. الـ non-
+
+128
+00:10:02,410 --> 00:10:06,350
+pathogenic ممكن أن تصنف تحتها harmless، أنها
+
+129
+00:10:06,350 --> 00:10:12,210
+لا تضر، وممكن أيضًا أن تصنف beneficial، أنها لها
+
+130
+00:10:12,210 --> 00:10:16,830
+فوائد.  في تصنيف ثالث، صنّفناها لـ
+
+131
+00:10:16,830 --> 00:10:19,950
+pathogenic، وهذا عدد قليل، non-pathogenic، معظم
+
+132
+00:10:19,950 --> 00:10:23,710
+الميكروبات. non-pathogenic، في تصنيف ثالث نقول
+
+133
+00:10:23,710 --> 00:10:27,070
+opportunistic pathogenic or non-pathogenic، يعني هذه البكتيريا
+
+134
+00:10:27,070 --> 00:10:31,330
+ممكن أن تكون ممرضة في أوقات، وممكن أن تكون غير ممرضة في
+
+135
+00:10:31,330 --> 00:10:36,410
+أوقات أخرى. طيب، على حسب ماذا تكون ممرضة، وعلى أي أساس
+
+136
+00:10:36,410 --> 00:10:41,050
+تكون غير ممرضة؟ نقول: حسب الـ immune system في الـ
+
+137
+00:10:41,050 --> 00:10:47,080
+host، حسب جهاز المناعة في العائل. الـ immune system لو
+
+138
+00:10:47,080 --> 00:10:51,160
+كان قويًا، لو كان الشخص سليمًا، يعني هذه البكتيريا non-pathogenic، غير ممرضة
+
+139
+00:10:51,160 --> 00:10:55,600
+لكن لو كان الشخص immunocompromised، يعني مناعته
+
+140
+00:11:00,780 --> 00:11:06,880
+ضعيفة، تكون pathogenic، تكون ممرضة. نرجع للفقرة
+
+141
+00:11:06,880 --> 00:11:12,190
+السابقة: Not all bacteria cause disease. هنا يقول لي أن
+
+142
+00:11:12,190 --> 00:11:16,870
+النسبة أقل من 10% من البكتيريا تسبب disease.
+
+143
+00:11:16,870 --> 00:11:21,370
+واتفقنا عليه، أن البكتيريا الـ pathogenic تكون نسبتها
+
+144
+00:11:21,370 --> 00:11:25,450
+قليلة. Many others are completely harmless. كثير
+
+145
+00:11:25,450 --> 00:11:31,450
+منها تعتبر harmless، يعني أنها لا تؤذي. واتفقنا أن
+
+146
+00:11:31,450 --> 00:11:35,780
+البكتيريا، النسبة أو النسبة الأكبر منها، non-
+
+147
+00:11:35,780 --> 00:11:39,740
+pathogenic، ممكن أن تكون harmless وممكن أن تكون أيضًا
+
+148
+00:11:39,740 --> 00:11:44,020
+beneficial. Some bacteria even do good things for
+
+149
+00:11:44,020 --> 00:11:48,780
+us, such as... صنّفناها لثلاث أصناف: الصنف
+
+150
+00:11:48,780 --> 00:11:54,040
+الأول هو pathogenic، وهي نسبة أقل من 10%. الصنف
+
+151
+00:11:54,040 --> 00:11:58,360
+الثاني قلنا هو non-pathogenic، ممكن أن تكون harmless
+
+152
+00:11:58,360 --> 00:12:03,750
+وممكن أن تكون beneficial. بالنسبة للبكتيريا، قلنا أنها ممكن
+
+153
+00:12:03,750 --> 00:12:07,450
+أن تكون ضمنها beneficial، لها فوائد. على سبيل المثال،
+
+154
+00:12:07,450 --> 00:12:11,850
+ذكرنا أن هناك بكتيريا تحوّل الحليب (milk) إلى
+
+155
+00:12:11,850 --> 00:12:17,170
+جبن. هذه البكتيريا اسمها الـ Lactobacillus. سنتعرف
+
+156
+00:12:17,170 --> 00:12:22,710
+معًا عليها، ترفع درجة حموضة البيئة قليلًا، يتحول الحليب
+
+157
+00:12:22,710 --> 00:12:28,530
+إلى جبن. من أيضًا ضمن البكتيريا التي لها فوائد،
+
+158
+00:12:28,530 --> 00:12:33,070
+هناك بكتيريا تصنع vitamin K في الأمعاء. في
+
+159
+00:12:33,070 --> 00:12:37,970
+بكتيريا تساعد في الهضم. في بكتيريا في أمعائنا
+
+160
+00:12:37,970 --> 00:12:42,330
+أمعائنا تعتبر normal flora. اسمها normal flora.
+
+161
+00:12:42,330 --> 00:12:45,510
+سنتعرف معًا عليها، بكتيريا موجودة بشكل طبيعي في جسم
+
+162
+00:12:45,510 --> 00:12:49,810
+الإنسان، لها أدوار كبيرة في العمليات الهضمية.
+
+164
+00:12:51,000 --> 00:12:55,440
+Bacteria often get a bad reputation as most people
+
+165
+00:12:55,440 --> 00:12:58,840
+tend to associate bacteria with disease. يقول أن
+
+166
+00:12:58,840 --> 00:13:02,560
+هناك سمعة سيئة للبكتيريا. البكتيريا
+
+167
+00:13:02,560 --> 00:13:09,060
+reputation معناها سمعة. هذه السمعة، يقول طبعًا سيئة
+
+168
+00:13:09,060 --> 00:13:13,950
+للـ bacteria، لأن الكثير منها يسبب لي أمراضًا، associated
+
+169
+00:13:13,950 --> 00:13:18,150
+bacteria with disease. الكثير منها يسبب لي أمراضًا، أكيد
+
+170
+00:13:18,150 --> 00:13:21,930
+هذه السمعة خاطئة، لأن قبل قليل ذكرنا أن فقط أقل من
+
+171
+00:13:21,930 --> 00:13:27,330
+عشرة بالمئة من البكتيريا يسبب لي أمراضًا. يقول أن
+
+172
+00:13:27,330 --> 00:13:32,110
+كثير من الناس يربطون البكتيريا بأنها ممرضة، لأن في
+
+173
+00:13:32,110 --> 00:13:36,950
+يوم من الأيام سببت لهم مرضًا، وأيضًا في بعض منهم كانت
+
+174
+00:13:36,950 --> 00:13:41,210
+مناعتهم ضعيفة وسببت لهم مرضًا، وكانت opportunistic، هي
+
+175
+00:13:41,210 --> 00:13:45,600
+because some of them caused disease. لأن بعض من
+
+176
+00:13:45,600 --> 00:
+
+223
+00:17:19,850 --> 00:17:20,210
+slide
diff --git a/PL9fwy3NUQKwZWgAb_V1ATlRRsH-6i3cPC/IO73Edx7DW0_postprocess.srt b/PL9fwy3NUQKwZWgAb_V1ATlRRsH-6i3cPC/IO73Edx7DW0_postprocess.srt
new file mode 100644
index 0000000000000000000000000000000000000000..58199d53887be8f97bdfe8bb294b663905d63749
--- /dev/null
+++ b/PL9fwy3NUQKwZWgAb_V1ATlRRsH-6i3cPC/IO73Edx7DW0_postprocess.srt
@@ -0,0 +1,892 @@
+1
+00:00:00,520 --> 00:00:03,960
+السلام عليكم ورحمة الله وبركاته يعطيكم العافية
+
+2
+00:00:03,960 --> 00:00:06,760
+جميعا في هذه المحاضرة ان شاء الله هنبدأ أول معمل
+
+3
+00:00:06,760 --> 00:00:10,840
+من مساق الـ General Microbiology Laboratory سابقا
+
+4
+00:00:10,840 --> 00:00:15,420
+عرفنا الـ General Microbiology it is the study of
+
+5
+00:00:15,420 --> 00:00:19,400
+microorganism هو العلم الذي يدرس الكائنات الدقيقة
+
+6
+00:00:19,400 --> 00:00:23,000
+ال micro organism اللي بندرسها في علم ال micro
+
+7
+00:00:23,000 --> 00:00:26,560
+biology it is small living organism that cannot be
+
+8
+00:00:26,560 --> 00:00:31,440
+seen by naked eye هي كائنات دقيقةحية لا ترى بالعين
+
+9
+00:00:31,440 --> 00:00:35,300
+المجردة تكلمنا أيضًا عن أقسام علم الـ Microbiology
+
+10
+00:00:35,300 --> 00:00:38,900
+وكان من ضمنها الـ Medical Microbiology المجال اللي
+
+11
+00:00:38,900 --> 00:00:43,560
+بنركز فيه دراستنا عليه كعملين في المجال الطبي it
+
+12
+00:00:43,560 --> 00:00:46,140
+is the study of microbes and they are associated
+
+13
+00:00:46,140 --> 00:00:50,280
+with host هو العلم اللي بيدرس الميكروبات و علاقتها
+
+14
+00:00:50,280 --> 00:00:55,220
+بالعائل ال host ال host اللي نقصد فيه الإنسان هل
+
+15
+00:00:55,220 --> 00:01:00,320
+هذا ال microbe بيسبب مرض لل hostهل الميكروب
+
+16
+00:01:00,320 --> 00:01:06,360
+harmless ما بدر هذا العائل أو هل الميكروب بيكون
+
+17
+00:01:06,360 --> 00:01:10,800
+مفيد للعائل beneficial كل هذا هنتعرف عليه الهدف من
+
+18
+00:01:10,800 --> 00:01:14,660
+مجلس البراتوري هو أن يتواجه الطلاب إلى مختلفة
+
+19
+00:01:14,660 --> 00:01:19,920
+الحياة في عالم الميكروبيا في هدفي من معمل الـ
+
+20
+00:01:19,920 --> 00:01:22,920
+microbiology أنه يكون عندي خلفية عن علم الأحياء
+
+21
+00:01:22,920 --> 00:01:26,800
+الدقيقة نتعرف على الميكروبات أكترفي المحاضرة
+
+22
+00:01:26,800 --> 00:01:31,040
+التعريفية حكينا إنه هدفي من معمل الـ Microbiology
+
+23
+00:01:31,040 --> 00:01:35,440
+إنه نعزل بكتيريا من عينات مرضية ونشخصها عبر فحوصات
+
+24
+00:01:35,440 --> 00:01:39,820
+مختلفة الجملتان نفس الشئ لأن حتى إن أنا أقدر أعزل
+
+25
+00:01:39,820 --> 00:01:43,420
+بكتيريا من عينات مرضية و أشخصها أكيد لازم يكون
+
+26
+00:01:43,420 --> 00:01:47,480
+عندي خلفية عن علم الأحياء الدقيقة أعرف خصائص كل
+
+27
+00:01:47,480 --> 00:01:51,850
+ميكروبأعرف الظروف اللي بينما فيها أعرف الأمراض
+
+28
+00:01:51,850 --> 00:01:56,710
+اللي بيسببها كل micro كل المعلومات اللي بتخص ال
+
+29
+00:01:56,710 --> 00:02:00,150
+micro بقى although the study of microbiology
+
+30
+00:02:00,150 --> 00:02:04,250
+include bacteria, virus, algae and protozoa this
+
+31
+00:02:04,250 --> 00:02:07,450
+lab will concentrate primarily on the bacteria
+
+32
+00:02:07,450 --> 00:02:12,230
+حكينا في علم ال medical microbiology هو علم واسع
+
+33
+00:02:12,230 --> 00:02:17,420
+بيدرس الكائنات الدقيقة منبكتيريا من فيروسات من
+
+34
+00:02:17,420 --> 00:02:22,920
+الجي طحالب and البروتوزوه البروتوزوه هي الأوليات
+
+35
+00:02:22,920 --> 00:02:28,440
+لكن قلنا في هذا المعمل ال essential medical
+
+36
+00:02:28,440 --> 00:02:32,800
+microbiology هنركز على البكتيريا طيب شو هي
+
+37
+00:02:32,800 --> 00:02:37,660
+البروتوزوه البروتوزوه معناها بالعربي هي الأوليات
+
+38
+00:02:37,660 --> 00:02:42,220
+طيب شو بتفرق عن البكتيريابقولنا الـ Protozoa هي
+
+39
+00:02:42,220 --> 00:02:47,600
+unicellular microorganism كائنات وحيدة الخلية
+
+40
+00:02:47,600 --> 00:02:52,480
+Eukaryotic شو يعني Eukaryotic يعني حقيقية النوع
+
+41
+00:02:52,480 --> 00:02:56,500
+طيب شو عكس ال Eukaryotic عكس ال Eukaryotic ال
+
+42
+00:02:56,500 --> 00:03:01,420
+Prokaryotic طيب شو هي البكتيريا البكتيريا هي عبارة
+
+43
+00:03:01,420 --> 00:03:06,240
+عن unicellular microorganism كائنات وحيدة الخلية
+
+44
+00:03:06,240 --> 00:03:11,210
+زي ال Protozoaلكن بتختلف عن البروتوزوه انها
+
+45
+00:03:11,210 --> 00:03:17,370
+بروكاريوتك بداية انه يبقى البروتوزوه unicellular
+
+46
+00:03:17,370 --> 00:03:22,690
+eukaryotic microorganism البكتيريا unicellular
+
+47
+00:03:22,690 --> 00:03:27,310
+بروكاريوتك microorganism بتشبه انهم unicellular
+
+48
+00:03:27,310 --> 00:03:32,710
+لكن بختلفوا ان البروتوزوه eukaryotic و البكتيريا
+
+49
+00:03:32,710 --> 00:03:37,940
+بروكاريوتكخلّينا نعرف شو الفرق بين الـ Eukaryotic
+
+50
+00:03:37,940 --> 00:03:43,240
+و البروكاريوتك البروكاريوتك طبعا هي بداية النواء
+
+51
+00:03:43,240 --> 00:03:48,080
+زي البكتيريا ال Eukaryotic هي حقيقية النواء زي
+
+52
+00:03:48,080 --> 00:03:53,640
+البروتوزوة زي مثلا الإنسان إحنا نعتبر Eukaryotic
+
+53
+00:03:53,640 --> 00:03:59,200
+لو بدنا نعمل مقارنة خلّينا أول وجه مقارنة نتكلم
+
+54
+00:03:59,200 --> 00:04:04,010
+على number of chromosomes عدد الكروموسوماتفي الـ
+
+55
+00:04:04,010 --> 00:04:08,650
+Eukaryotic احنا الإنسان عدد الكرموسومات 46 كرموسوم
+
+56
+00:04:08,650 --> 00:04:13,850
+لكن الـ Prokaryotic زي البكتيريا تتكون من one
+
+57
+00:04:13,850 --> 00:04:19,410
+كرموسوم يبقى أول وجه مقارنة بالنسبة ل number of
+
+58
+00:04:19,410 --> 00:04:24,350
+chromosomes طيب shape of chromosome لو قارننا شكل
+
+59
+00:04:24,350 --> 00:04:28,970
+الكرموسوم بين الـ Prokaryotic و الـ Eukaryotic الـ
+
+60
+00:04:28,970 --> 00:04:33,940
+Eukaryotic الإنسان يعتبر شكل الكرموسوم linearلكن
+
+61
+00:04:33,940 --> 00:04:39,300
+الـ Prokaryotic يُعتبر Circular هاي تاني وجه
+
+62
+00:04:39,300 --> 00:04:43,800
+مقارنة الـ shape of a chromosome الـ Prokaryotic
+
+63
+00:04:43,800 --> 00:04:48,620
+يُعتبر Circular لكن الـ Eukaryotic يُعتبر Linear
+
+64
+00:04:48,620 --> 00:04:55,340
+لو حكينا عن ال mutation rate، معدل حدوث الطفرات
+
+65
+00:04:55,340 --> 00:04:59,840
+الـ Prokaryotic بقولك إن معدل حدوث الطفرات فيها
+
+66
+00:04:59,840 --> 00:05:05,740
+تُعتبر high rateلكن الـ Eukaryotic تعتبر low rate
+
+67
+00:05:05,740 --> 00:05:09,980
+طيب لو اتكلمنا عن السبب اللي بيخلي ان معدل حدوث
+
+68
+00:05:09,980 --> 00:05:14,900
+الطفرات في الـ Eukaryotic أقل من الـ Prokaryotic
+
+69
+00:05:15,630 --> 00:05:19,410
+الـ Eukaryotic حقيقية انه هو زي الإنسان احنا بنعرف
+
+70
+00:05:19,410 --> 00:05:23,550
+في كل مرحلة من مراحل ال cell ال cell cycle بيصير
+
+71
+00:05:23,550 --> 00:05:26,950
+في يدي تصحيح لأي طفرة لأنه في يدي checkpoint
+
+72
+00:05:26,950 --> 00:05:31,490
+بالتالي أكيد فرصة حدوث الطفرات في الـ Eukaryotic
+
+73
+00:05:31,490 --> 00:05:39,010
+أقل هذا وجه المقارنة التالت لـ mutation rate طبعا
+
+74
+00:05:39,010 --> 00:05:45,050
+من أوجه المقارنة المهمة أيضاحتى نفرق بين الـ
+
+75
+00:05:45,050 --> 00:05:49,410
+Prokaryotic و Eukaryotic الريبوسوم الريبوسوم هو
+
+76
+00:05:49,410 --> 00:05:54,530
+مصنع البروتين في الخلية سواء كان الـ Prokaryotic
+
+77
+00:05:54,530 --> 00:05:59,870
+أو الـ Eukaryotic الريبوسوم بتكون من قطعتين قطعة
+
+78
+00:05:59,870 --> 00:06:04,630
+صغيرة و قطعة كبيرة الاختلاف يكون في سرعه الترسيب
+
+79
+00:06:04,630 --> 00:06:08,850
+كل من هذه القطعتين و أيضا سرعه الترسيب الريبوسوم
+
+80
+00:06:08,850 --> 00:06:13,630
+بقولنا في الـ Prokaryoticلما جبنا الـ ribosome و
+
+81
+00:06:13,630 --> 00:06:17,950
+روحنا رسّبنا في جهاز الـ centrifuge لقوا سرعة
+
+82
+00:06:17,950 --> 00:06:24,070
+الترسيب 70 أس لكن لما فصلنا القطعة الكبيرة لحال و
+
+83
+00:06:24,070 --> 00:06:27,730
+القطعة الصغيرة لحال القطعة الكبيرة كانت سرعة
+
+84
+00:06:27,730 --> 00:06:33,830
+الترسيب 50 أس و القطعة الصغيرة كانت 30 أس هذا
+
+85
+00:06:33,830 --> 00:06:38,130
+بالنسبة لـ Sedimentation Rate أما بالنسبة لـ
+
+86
+00:06:38,130 --> 00:06:42,280
+Eukaryoticلما رسّبنا الـ ribosome بشكل كامل في
+
+87
+00:06:42,280 --> 00:06:47,680
+جهاز الـ centrifuge لقينا أنه بترسب على سرعة 80S
+
+88
+00:06:47,680 --> 00:06:52,460
+لما فصلنا القطعة الكبيرة عن القطعة الصغيرة القطعة
+
+89
+00:06:52,460 --> 00:06:57,700
+الكبيرة كانت سرعة الترسيب 60S أما القطعة الصغيرة
+
+90
+00:06:57,700 --> 00:07:05,410
+كانت 40S تمامهدول أوجه المقارنة بين الـ
+
+91
+00:07:05,410 --> 00:07:08,670
+Prokaryotic و الـ Eukaryotic طبعا فين دي أوجه
+
+92
+00:07:08,670 --> 00:07:14,110
+مقارنة أخرى لكن هاي أهم أوجه المقارنة احنا قلنا في
+
+93
+00:07:14,110 --> 00:07:18,270
+sedimentation rate ان سرعة الترسيب لبروكاريوتك
+
+94
+00:07:18,270 --> 00:07:23,650
+الريبوسوم بشكل كامل 70 أس طيب إيش هي الأس؟ الأس هي
+
+95
+00:07:23,650 --> 00:07:29,590
+اختصار للـ pseudoband هي عبارة عن وحدة قياس بتقيص
+
+96
+00:07:29,590 --> 00:07:34,610
+ال rate of sedimentation يبقىلو بنعرف الـ Sudberg
+
+97
+00:07:34,610 --> 00:07:39,350
+as the unit measures the rate of sedimentation
+
+98
+00:07:39,350 --> 00:07:44,030
+rather than size هي واحدة بتقيس معدل سرعي الترسيب
+
+99
+00:07:44,030 --> 00:07:49,270
+للبروتين مش حجم البروتين اللي بترسب طب أنا ليش
+
+100
+00:07:49,270 --> 00:07:52,670
+قولت انه مش حجم البروتين اللي ترسب لأنه احنا قلنا
+
+101
+00:07:52,670 --> 00:07:56,030
+لما رسبنا القطعة الكبيرة كانت في الـ Broker New
+
+102
+00:07:56,030 --> 00:08:00,230
+Tech كانت سرعي الترسيب 50 أس لما رسبنا القطعة
+
+103
+00:08:00,230 --> 00:08:04,620
+الصغيرةكانت سرعة الترسيب 30 أس لكن لما رسّبنا
+
+104
+00:08:04,620 --> 00:08:09,580
+الريبوسومب كامل كان 70 ماكان مجموحهم 80 يبقى احنا
+
+105
+00:08:09,580 --> 00:08:13,440
+ما بنجمع هذه السرعة اللي بترسب عندها ال protein
+
+106
+00:08:13,440 --> 00:08:19,740
+ككل تمام هذا بالنسبة لأوجه المقارنة بين ال
+
+107
+00:08:19,740 --> 00:08:22,320
+biokaryotic و الeokaryotic
+
+108
+00:08:25,080 --> 00:08:29,720
+بكتيريا are unicellular prokaryotic microorganism
+
+109
+00:08:29,720 --> 00:08:34,560
+اتكلمنا ان هي عبارة عن unicellular وحيدة الخلية
+
+110
+00:08:34,560 --> 00:08:39,560
+prokaryotic بداية ان هو micro organism طبعا لو انا
+
+111
+00:08:39,560 --> 00:08:43,440
+غيرته حاجات unicellular ايوكاريوتيك micro organism
+
+112
+00:08:43,440 --> 00:08:49,610
+بتكلم عن proto zoathat divide by binary effusion
+
+113
+00:08:49,610 --> 00:08:54,250
+بتنقسم بواسطة الانشطار الصناعي أو binary division
+
+114
+00:08:54,250 --> 00:09:00,210
+نفس الكلمة هان بعرفلي ال binary effusion a process
+
+115
+00:09:00,210 --> 00:09:04,850
+by which one bacterium split into two ان الخلية
+
+116
+00:09:04,850 --> 00:09:11,070
+البكتيرية الواحدة بتنقسم split to two لأ خليتين
+
+117
+00:09:11,070 --> 00:09:14,730
+بكتيريتين هدول الخليتين البكتيريتين بيكونوا
+
+118
+00:09:14,730 --> 00:09:19,790
+identicalوبيكونوا typical عن الخلية الأصل طيب
+
+119
+00:09:19,790 --> 00:09:24,890
+بالنسبة للبكتيريا كتير ناس لما تسمع كلمة بكتيريا
+
+120
+00:09:24,890 --> 00:09:29,310
+أول ما بخطر ببالها ان إشي هي dark لكن الحقيقة مش
+
+121
+00:09:29,310 --> 00:09:34,430
+هيك الميكروبات بشكل عام ممكن تكون pathogenic وممكن
+
+122
+00:09:34,430 --> 00:09:38,630
+تكون non pathogenic ال non pathogenic يعني غير
+
+123
+00:09:38,630 --> 00:09:44,550
+ممرضة وهذا معظم الميكروبات بتتصنف منها تعتبر non
+
+124
+00:09:44,550 --> 00:09:49,310
+pathogenicلكن ال pathogenic microbe اللي هي اللي
+
+125
+00:09:49,310 --> 00:09:52,790
+ممرضة مايعني pathogenic يعني أنها بتعمل disease
+
+126
+00:09:52,790 --> 00:09:57,570
+نسبة قليلة من ال micro part تعتبر pathogenic يبقى
+
+127
+00:09:57,570 --> 00:10:02,410
+النسبة الأكبر أو المعظم تكون non pathogenic ال non
+
+128
+00:10:02,410 --> 00:10:06,350
+pathogenic ممكن أنها تكون تصنف تحتها harmless إنها
+
+129
+00:10:06,350 --> 00:10:12,210
+ما بتضر وممكن كمان إنها تصنف beneficial إنها إلها
+
+130
+00:10:12,210 --> 00:10:16,830
+فوائدفي عندى برضه تصنيف تالت احنا صنفناها لأ
+
+131
+00:10:16,830 --> 00:10:19,950
+pathogenic وهذا عدد قليل non pathogenic معظم
+
+132
+00:10:19,950 --> 00:10:23,710
+الميكروباط non pathogenic في عندى صنف تالت بقولك
+
+133
+00:10:23,710 --> 00:10:27,070
+pathogenic or non pathogenic يعني هذه البكتيريا
+
+134
+00:10:27,070 --> 00:10:31,330
+ممكن تكون ممرضة في أوقات وممكن تكون غير ممرضة في
+
+135
+00:10:31,330 --> 00:10:36,410
+أوقات أخرى طيب على حسب شو بتكون ممرضة وعلى أي أساس
+
+136
+00:10:36,410 --> 00:10:41,050
+بتكون غير ممرضة بقولنا حسب ال immune system في ال
+
+137
+00:10:41,050 --> 00:10:47,080
+host حسبجهاز المناعة في العقل الـ immune system لو
+
+138
+00:10:47,080 --> 00:10:51,160
+كان الشخص لو كان ال immune system ميح وكان الشخص
+
+139
+00:10:51,160 --> 00:10:55,600
+صليم يعني هذه البكتيريا non pathogenic غير ممرضة
+
+140
+00:10:55,600 --> 00:11:00,780
+لكن لو كان الشخص immunocompromised يعني منعته
+
+141
+00:11:00,780 --> 00:11:06,880
+ضعيفة بتكون pathogenic بتكون ممرضة نرجع لأ الفقرة
+
+142
+00:11:06,880 --> 00:11:12,190
+للذنبof all bacteria cause disease هان بقوللي إنه
+
+143
+00:11:12,190 --> 00:11:16,870
+النسبة أقل من 10% من البكتيريا بتسبب disease
+
+144
+00:11:16,870 --> 00:11:21,370
+واتفقنا عليه إنه ال pathogenic bacteria بتكون نسبة
+
+145
+00:11:21,370 --> 00:11:25,450
+قليلة many others are completely harmless كتير
+
+146
+00:11:25,450 --> 00:11:31,450
+منها تعتبر harmless يعني إنها ما بتأذي واتفقنا إنه
+
+147
+00:11:31,450 --> 00:11:35,780
+البكتيريا النسبة أو النسبة الأكبرإنها non
+
+148
+00:11:35,780 --> 00:11:39,740
+-pathogenic ممكن تكون harmless وممكن تكون أيضا
+
+149
+00:11:39,740 --> 00:11:44,020
+beneficial some bacteria even do good things for
+
+150
+00:11:44,020 --> 00:11:48,780
+us such as Turnbull كيوم صنفليها لتلت أصناف الصنف
+
+151
+00:11:48,780 --> 00:11:54,040
+الأول هي pathogenic وهي نسبة أقل من 10% الصنف
+
+152
+00:11:54,040 --> 00:11:58,360
+التاني قلنا هي non-pathogenic ممكن تكون harmless
+
+153
+00:11:58,360 --> 00:12:03,750
+وممكن تكون beneficialبنسبة للبكتيريا قلنا إنه ممكن
+
+154
+00:12:03,750 --> 00:12:07,450
+تكون صمنها beneficial إلها فوائد على سبيل المثال
+
+155
+00:12:07,450 --> 00:12:11,850
+ذاكر إنه في عيناه بكتيريا بتحول الحليب الـ milk to
+
+156
+00:12:11,850 --> 00:12:17,170
+الجبنة هذه البكتيريا اسمها الـ lactobacillus هتمر
+
+157
+00:12:17,170 --> 00:12:22,710
+معنا بترفع درجة الحموضة البيئة شوي بيتحول الحليب
+
+158
+00:12:22,710 --> 00:12:28,530
+لجبنةمن أيضا في من ضمن البكتيريا اللي إلها فوائد
+
+159
+00:12:28,530 --> 00:12:33,070
+في بكتيريا عندنا بتصنع vitamin K في الأمعاق في
+
+160
+00:12:33,070 --> 00:12:37,970
+بكتيريا عندنا بتخدم القليل في بكتيريا في أمعاق
+
+161
+00:12:37,970 --> 00:12:42,330
+أمعاقنا تعتبر normal flora اسمها normal flora
+
+162
+00:12:42,330 --> 00:12:45,510
+هتمور معاكم بكتيريا موجودة بشكل طبيعي في جسم
+
+163
+00:12:45,510 --> 00:12:49,810
+الإنسان إلها أدوار كبيرة في العمليات الإيدي
+
+164
+00:12:51,000 --> 00:12:55,440
+Bacteria often get a bad reputation as most people
+
+165
+00:12:55,440 --> 00:12:58,840
+tend to associate bacteria with disease بقولنا إن
+
+166
+00:12:58,840 --> 00:13:02,560
+هنا في سمعة سيئة على البكتيريا البكتيريا
+
+167
+00:13:02,560 --> 00:13:09,060
+reputation معناها سمعة هذه السمعة بقولك طبعا سيئة
+
+168
+00:13:09,060 --> 00:13:13,950
+لأ البكتيريا لأن كتير منها بيسببلي أمراضassociated
+
+169
+00:13:13,950 --> 00:13:18,150
+بكتيريا wet disease كتير منها بيسببلي أمراض أكيد
+
+170
+00:13:18,150 --> 00:13:21,930
+هاي السمع غلط لأنه قبل شوية ذكرنا أنه فقط أقل من
+
+171
+00:13:21,930 --> 00:13:27,330
+عشرة بالمية من البكتيريا بيسببلي أمراض بقول إنه
+
+172
+00:13:27,330 --> 00:13:32,110
+كتير من الناس بربطوا البكتيريا بأنها ممرضة لأنه في
+
+173
+00:13:32,110 --> 00:13:36,950
+يوم من الأيام سببت لهم مرض وأيضا في بعض منهم كانت
+
+174
+00:13:36,950 --> 00:13:41,210
+مناعتهم ضعيفة وسببت لهم مرض وكانت abortionist هي
+
+175
+00:13:41,210 --> 00:13:45,600
+because some of themcaused disease لأن بعض من
+
+176
+00:13:45,600 --> 00:13:50,880
+الناس سببت لهم مرض and some other وناس آخرين are
+
+177
+00:13:50,880 --> 00:13:53,800
+abortionistic كانت بالنسبة لإلهم البكتيريا
+
+178
+00:13:53,800 --> 00:13:59,120
+انتهازية انتهازية يعني استغلت ضعف جهاز المناع
+
+179
+00:13:59,120 --> 00:14:05,220
+بقولك البكتيريا الانتهازية هاي فقط that is that is
+
+180
+00:14:05,220 --> 00:14:09,540
+they can cause disease in an ill or injured person
+
+181
+00:14:09,960 --> 00:14:13,640
+هذه البكتيريا الانتهازية بتعمل فقط مرض في الإنسان
+
+182
+00:14:13,640 --> 00:14:17,540
+المريض لما يكون إن الإنسان في عنده ضعف جهاز المناع
+
+183
+00:14:17,540 --> 00:14:21,960
+لما يكون الإنسان مريض هذه الـ abortionistic
+
+184
+00:14:21,960 --> 00:14:26,360
+بكتيريا بتنتهز الفرصة و بتسبب مرض للإنسان many
+
+185
+00:14:26,360 --> 00:14:30,400
+bacteria cannot even live at the temperature found
+
+186
+00:14:30,400 --> 00:14:33,820
+in and on the human body في كتير من البكتيريا ما
+
+187
+00:14:33,820 --> 00:14:38,210
+بتقدر تعيش بدرجة حرارة جسم الإنسانلازم حتى اني انا
+
+188
+00:14:38,210 --> 00:14:43,330
+اعزلها اوفرلها الظروف المناسبة bacteria are
+
+189
+00:14:43,330 --> 00:14:47,070
+everywhere اتكلمنا ان البكتيريا موجودة في كل مكان
+
+190
+00:14:47,070 --> 00:14:51,270
+موجودة على الأسطح موجودة في التربة موجودة على
+
+191
+00:14:51,270 --> 00:14:54,850
+جسمنا on every surface of the body موجودة على كل
+
+192
+00:14:54,850 --> 00:14:58,850
+سطح في جسمنا في وجهنا على أيدينا في كل مكان في
+
+193
+00:14:58,850 --> 00:15:03,190
+جسمنا موجودة البكتيرياincluding digestive tract
+
+194
+00:15:03,190 --> 00:15:06,390
+كذلك موجودة في الـ digestive tract أكيد حتى إنها
+
+195
+00:15:06,390 --> 00:15:11,090
+تساعد في هضم الطعام صنفنا البكتيريا إنها harmless
+
+196
+00:15:11,090 --> 00:15:17,210
+غير مؤذية beneficial طبعا مفيدة هدولة الصنفين
+
+197
+00:15:17,210 --> 00:15:21,470
+يعتبروا non pathogenic غير مضرة وهي نسبة الأكتر
+
+198
+00:15:21,470 --> 00:15:26,290
+pathogenic بكتيريا بتعمل أمراض وهي أقل من 10% من
+
+199
+00:15:26,290 --> 00:15:31,600
+البكتيريابقولك الـ bacteria absorbs nutrients and
+
+200
+00:15:31,600 --> 00:15:34,100
+releases toxins that damage cell and tissue
+
+201
+00:15:34,100 --> 00:15:37,940
+البكتيريا بتنطص المواد الغذائية أكيد بتنطص حتى
+
+202
+00:15:37,940 --> 00:15:41,480
+تقدر تنمو وتتكثر و تكون قادرة على إحداث المرض
+
+203
+00:15:41,480 --> 00:15:46,140
+وأيضا بتفرز توكسين بتفرز السموم السموم تعتبر
+
+204
+00:15:46,140 --> 00:15:49,940
+كviolence factor هنسمع هذه الكلمة الviolence
+
+205
+00:15:49,940 --> 00:15:54,520
+factor معناها عوامل شراسة بتستخدمها البكتيريا حتى
+
+206
+00:15:54,520 --> 00:15:58,870
+إنها تكون قادرة على إحداث المرضبتدمر الخلايا و
+
+207
+00:15:58,870 --> 00:16:03,750
+الانسجار بالنسبة للسموم التوكسين السموم اللى
+
+208
+00:16:03,750 --> 00:16:08,990
+بتفرزها البكتيريا فى عندنا أنواع لهذه التوكسين
+
+209
+00:16:08,990 --> 00:16:14,150
+ممكن تكون اندوتوكسين و ممكن تكون اكسوتوكسين طيب شو
+
+210
+00:16:14,150 --> 00:16:18,990
+الفرق بين الاندوتوكسين و الاكسوتوكسين الاندوتوكسين
+
+211
+00:16:18,990 --> 00:16:24,010
+موجود من اسمه داخل البكتيريا يعنيلو ماتت البكتيريا
+
+212
+00:16:24,010 --> 00:16:28,110
+راح يطلع هذا التوكسين ويأثر على الخلايا و الانسجا
+
+213
+00:16:28,110 --> 00:16:33,210
+ويدمر هذا العائل الـ host يبقى بنحكي الـ Endotoxin
+
+214
+00:16:33,210 --> 00:16:38,870
+تفرزه البكتيريا و هي ميتة from dead bacteria أما
+
+215
+00:16:38,870 --> 00:16:44,590
+ال Exotoxin خارجي بتفرزه البكتيريا و هي حية from
+
+216
+00:16:44,590 --> 00:16:48,420
+living bacteriaيبقى ال endotoxin from dead
+
+217
+00:16:48,420 --> 00:16:53,260
+bacteria لكن ال exotoxin from living bacteria عشان
+
+218
+00:16:53,260 --> 00:16:58,900
+هيك لو إنسان مصاب ببكتيريا بتفرز ال endotoxin
+
+219
+00:16:58,900 --> 00:17:04,440
+الدكتور في هذه الحالة مابيعطيه antibiotic بيقتل
+
+220
+00:17:04,440 --> 00:17:08,000
+اللي هو البكتيريا لأنه لو أعطاه antibiotic بيقتل
+
+221
+00:17:08,000 --> 00:17:13,310
+البكتيرياهيتفرز الـ bacterial endotoxin ويتدمر
+
+222
+00:17:13,310 --> 00:17:19,850
+الخلايا ويتأثر على الـ host هذا بالنسبة لهذا الـ
+
+223
+00:17:19,850 --> 00:17:20,210
+slide
+
diff --git a/PL9fwy3NUQKwZWgAb_V1ATlRRsH-6i3cPC/IO73Edx7DW0_raw.json b/PL9fwy3NUQKwZWgAb_V1ATlRRsH-6i3cPC/IO73Edx7DW0_raw.json
new file mode 100644
index 0000000000000000000000000000000000000000..6f72535859c14932bf47ba96d70963e1789122ef
--- /dev/null
+++ b/PL9fwy3NUQKwZWgAb_V1ATlRRsH-6i3cPC/IO73Edx7DW0_raw.json
@@ -0,0 +1 @@
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0.9781494140625}, {"start": 239.2, "end": 239.62, "word": " على", "probability": 0.61376953125}, {"start": 239.62, "end": 240.22, "word": " number", "probability": 0.52978515625}, {"start": 240.22, "end": 240.64, "word": " of", "probability": 0.97998046875}, {"start": 240.64, "end": 241.36, "word": " chromosomes", "probability": 0.16162109375}, {"start": 241.36, "end": 241.98, "word": " عدد", "probability": 0.9475911458333334}, {"start": 241.98, "end": 242.96, "word": " الكروموسومات", "probability": 0.7421061197916666}], "temperature": 1.0}, {"id": 10, "seek": 27277, "start": 243.49, "end": 272.77, "text": "في الـ Eukaryotic احنا الإنسان عدد الكرموسومات 46 كرموسوم لكن الـ Prokaryotic زي البكتيريا تتكون من one كرموسوم يبقى أول وجه مقارنة بالنسبة ل number of chromosomes طيب shape of chromosome لو قارننا شكل الكرموسوم بين الـ Prokaryotic و الـ Eukaryotic الـ Eukaryotic الإنسان يعتبر شكل الكرموسوم linear", "tokens": [41185, 2423, 39184, 462, 2034, 822, 9411, 1975, 5016, 8315, 33688, 1863, 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244.01, "end": 244.59, "word": " Eukaryotic", "probability": 0.84423828125}, {"start": 244.59, "end": 244.91, "word": " احنا", "probability": 0.7898763020833334}, {"start": 244.91, "end": 245.57, "word": " الإنسان", "probability": 0.97314453125}, {"start": 245.57, "end": 245.95, "word": " عدد", "probability": 0.9847005208333334}, {"start": 245.95, "end": 247.01, "word": " الكرموسومات", "probability": 0.8106282552083334}, {"start": 247.01, "end": 247.49, "word": " 46", "probability": 0.88818359375}, {"start": 247.49, "end": 248.65, "word": " كرموسوم", "probability": 0.93955078125}, {"start": 248.65, "end": 249.39, "word": " لكن", "probability": 0.82177734375}, {"start": 249.39, "end": 249.75, "word": " الـ", "probability": 0.884033203125}, {"start": 249.75, "end": 250.61, "word": " Prokaryotic", "probability": 0.8525390625}, {"start": 250.61, "end": 251.23, "word": " زي", "probability": 0.942626953125}, {"start": 251.23, "end": 252.07, "word": " البكتيريا", "probability": 0.97119140625}, {"start": 252.07, "end": 252.89, "word": " تتكون", "probability": 0.94873046875}, {"start": 252.89, "end": 253.33, "word": " من", "probability": 0.9970703125}, {"start": 253.33, "end": 253.85, "word": " one", "probability": 0.61767578125}, {"start": 253.85, "end": 254.71, "word": " كرموسوم", "probability": 0.8654296875}, {"start": 254.71, "end": 255.15, "word": " يبقى", "probability": 0.9608154296875}, {"start": 255.15, "end": 255.49, "word": " أول", "probability": 0.91552734375}, {"start": 255.49, "end": 255.83, "word": " وجه", "probability": 0.8974609375}, {"start": 255.83, "end": 256.69, "word": " مقارنة", "probability": 0.99189453125}, {"start": 256.69, "end": 258.27, "word": " بالنسبة", "probability": 0.98193359375}, {"start": 258.27, "end": 258.47, "word": " ل", "probability": 0.48974609375}, {"start": 258.47, "end": 258.93, "word": " number", "probability": 0.50390625}, {"start": 258.93, "end": 259.41, "word": " of", "probability": 0.9697265625}, {"start": 259.41, "end": 260.21, "word": " chromosomes", "probability": 0.298583984375}, {"start": 260.21, "end": 261.05, "word": " طيب", "probability": 0.8645833333333334}, {"start": 261.05, "end": 261.93, "word": " shape", "probability": 0.313232421875}, {"start": 261.93, "end": 262.31, "word": " of", "probability": 0.9755859375}, {"start": 262.31, "end": 263.05, "word": " chromosome", "probability": 0.509765625}, {"start": 263.05, "end": 263.35, "word": " لو", "probability": 0.95556640625}, {"start": 263.35, "end": 263.89, "word": " قارننا", "probability": 0.924560546875}, {"start": 263.89, "end": 264.35, "word": " شكل", "probability": 0.986328125}, {"start": 264.35, "end": 265.29, "word": " الكرموسوم", "probability": 0.9384765625}, {"start": 265.29, "end": 265.55, "word": " بين", "probability": 0.95703125}, {"start": 265.55, "end": 265.83, "word": " الـ", "probability": 0.6524658203125}, {"start": 265.83, "end": 266.53, "word": " Prokaryotic", "probability": 0.9532470703125}, {"start": 266.53, "end": 266.79, "word": " و", "probability": 0.923828125}, {"start": 266.79, "end": 267.11, "word": " الـ", "probability": 0.5543212890625}, {"start": 267.11, "end": 267.95, "word": " Eukaryotic", "probability": 0.9407958984375}, {"start": 267.95, "end": 268.97, "word": " الـ", "probability": 0.826416015625}, {"start": 268.97, "end": 269.63, "word": " Eukaryotic", "probability": 0.9703369140625}, {"start": 269.63, "end": 270.39, "word": " الإنسان", "probability": 0.9703369140625}, {"start": 270.39, "end": 271.03, "word": " يعتبر", "probability": 0.8458658854166666}, {"start": 271.03, "end": 271.45, "word": " شكل", "probability": 0.988525390625}, {"start": 271.45, "end": 272.37, "word": " الكرموسوم", "probability": 0.9595703125}, {"start": 272.37, "end": 272.77, "word": " linear", "probability": 0.58740234375}], "temperature": 1.0}, {"id": 11, "seek": 30138, "start": 273.32, "end": 301.38, "text": "لكن الـ Prokaryotic يُعتبر Circular هاي تاني وجه مقارنة الـ shape of a chromosome الـ Prokaryotic يُعتبر Circular لكن الـ Eukaryotic يُعتبر Linear لو حكينا عن ال mutation rate، معدل حدوث الطفرات الـ Prokaryotic بقولك إن معدل حدوث الطفرات فيها تُعتبر high rate", "tokens": [1211, 19452, 2423, 39184, 1705, 74, 822, 9411, 7251, 10859, 34268, 26890, 13791, 17792, 8032, 47302, 6055, 7649, 1829, 49610, 3224, 3714, 4587, 9640, 1863, 3660, 2423, 39184, 3909, 295, 257, 42896, 2423, 39184, 1705, 74, 822, 9411, 7251, 10859, 34268, 26890, 13791, 17792, 44381, 2423, 39184, 462, 2034, 822, 9411, 7251, 10859, 34268, 26890, 14670, 289, 45164, 11331, 4117, 1829, 8315, 18871, 2423, 27960, 3314, 12399, 20449, 3215, 1211, 11331, 3215, 2407, 12984, 41950, 5172, 2288, 9307, 2423, 39184, 1705, 74, 822, 9411, 4724, 39648, 4117, 36145, 20449, 3215, 1211, 11331, 3215, 2407, 12984, 41950, 5172, 2288, 9307, 8978, 11296, 6055, 10859, 34268, 26890, 1090, 3314], "avg_logprob": -0.15104166915019354, "compression_ratio": 1.8284313725490196, "no_speech_prob": 1.1563301086425781e-05, "words": 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{"start": 281.72, "end": 281.94, "word": " a", "probability": 0.91357421875}, {"start": 281.94, "end": 282.62, "word": " chromosome", "probability": 0.8740234375}, {"start": 282.62, "end": 283.22, "word": " الـ", "probability": 0.5299072265625}, {"start": 283.22, "end": 283.8, "word": " Prokaryotic", "probability": 0.97265625}, {"start": 283.8, "end": 284.38, "word": " يُعتبر", "probability": 0.9793701171875}, {"start": 284.38, "end": 285.12, "word": " Circular", "probability": 0.865478515625}, {"start": 285.12, "end": 285.92, "word": " لكن", "probability": 0.85791015625}, {"start": 285.92, "end": 286.22, "word": " الـ", "probability": 0.87109375}, {"start": 286.22, "end": 286.84, "word": " Eukaryotic", "probability": 0.9300537109375}, {"start": 286.84, "end": 287.52, "word": " يُعتبر", "probability": 0.9910888671875}, {"start": 287.52, "end": 288.62, "word": " Linear", "probability": 0.901123046875}, {"start": 288.62, "end": 290.04, "word": " لو", "probability": 0.96826171875}, {"start": 290.04, "end": 291.56, "word": " حكينا", "probability": 0.9945068359375}, {"start": 291.56, "end": 291.94, "word": " عن", "probability": 0.9921875}, {"start": 291.94, "end": 292.22, "word": " ال", "probability": 0.8486328125}, {"start": 292.22, "end": 292.82, "word": " mutation", "probability": 0.66259765625}, {"start": 292.82, "end": 293.42, "word": " rate،", "probability": 0.6102294921875}, {"start": 293.42, "end": 293.96, "word": " معدل", "probability": 0.91357421875}, {"start": 293.96, "end": 294.52, "word": " حدوث", "probability": 0.9892578125}, {"start": 294.52, "end": 295.34, "word": " الطفرات", "probability": 0.8895263671875}, {"start": 295.34, "end": 296.3, "word": " الـ", "probability": 0.91259765625}, {"start": 296.3, "end": 297.12, "word": " Prokaryotic", "probability": 0.982666015625}, {"start": 297.12, "end": 297.56, "word": " بقولك", "probability": 0.88916015625}, {"start": 297.56, "end": 297.84, "word": " إن", "probability": 0.65234375}, {"start": 297.84, "end": 298.64, "word": " معدل", "probability": 0.9117838541666666}, {"start": 298.64, "end": 299.02, "word": " حدوث", "probability": 0.978515625}, {"start": 299.02, "end": 299.56, "word": " الطفرات", "probability": 0.9910888671875}, {"start": 299.56, "end": 299.84, "word": " فيها", "probability": 0.966064453125}, {"start": 299.84, "end": 300.46, "word": " تُعتبر", "probability": 0.809326171875}, {"start": 300.46, "end": 301.02, "word": " high", "probability": 0.81640625}, {"start": 301.02, "end": 301.38, "word": " rate", "probability": 0.94775390625}], "temperature": 1.0}, {"id": 12, "seek": 31489, "start": 301.66, "end": 314.9, "text": "لكن الـ Eukaryotic تعتبر low rate طيب لو اتكلمنا عن السبب اللي بيخلي ان معدل حدوث الطفرات في الـ Eukaryotic أقل من الـ Prokaryotic", "tokens": [1211, 19452, 2423, 39184, 462, 2034, 822, 9411, 6055, 34268, 26890, 2295, 3314, 23032, 1829, 3555, 45164, 1975, 2655, 4117, 19528, 8315, 18871, 21136, 3555, 3555, 13672, 1829, 4724, 1829, 9778, 20292, 16472, 20449, 3215, 1211, 11331, 3215, 2407, 12984, 41950, 5172, 2288, 9307, 8978, 2423, 39184, 462, 2034, 822, 9411, 5551, 4587, 1211, 9154, 2423, 39184, 1705, 74, 822, 9411], "avg_logprob": -0.15990422834311763, "compression_ratio": 1.3916083916083917, "no_speech_prob": 1.3113021850585938e-06, "words": [{"start": 301.66, "end": 302.36, "word": "لكن", "probability": 0.816162109375}, {"start": 302.36, "end": 302.76, "word": " الـ", "probability": 0.769287109375}, {"start": 302.76, "end": 303.48, "word": " Eukaryotic", "probability": 0.85467529296875}, {"start": 303.48, "end": 304.2, "word": " تعتبر", "probability": 0.91748046875}, {"start": 304.2, "end": 304.92, "word": " low", "probability": 0.10980224609375}, {"start": 304.92, "end": 305.74, "word": " rate", "probability": 0.763671875}, {"start": 305.74, "end": 306.12, "word": " طيب", "probability": 0.8909505208333334}, {"start": 306.12, "end": 306.44, "word": " لو", "probability": 0.9365234375}, {"start": 306.44, "end": 307.2, "word": " اتكلمنا", "probability": 0.91142578125}, {"start": 307.2, "end": 307.4, "word": " عن", "probability": 0.9931640625}, {"start": 307.4, "end": 307.96, "word": " السبب", "probability": 0.98974609375}, {"start": 307.96, "end": 308.2, "word": " اللي", "probability": 0.89599609375}, {"start": 308.2, "end": 308.78, "word": " بيخلي", "probability": 0.896484375}, {"start": 308.78, "end": 309.12, "word": " ان", "probability": 0.67236328125}, {"start": 309.12, "end": 309.58, "word": " معدل", "probability": 0.92529296875}, {"start": 309.58, "end": 309.98, "word": " حدوث", "probability": 0.9642333984375}, {"start": 309.98, "end": 310.78, "word": " الطفرات", "probability": 0.9132080078125}, {"start": 310.78, "end": 311.28, "word": " في", "probability": 0.9609375}, {"start": 311.28, "end": 312.04, "word": " الـ", "probability": 0.853271484375}, {"start": 312.04, "end": 312.84, "word": " Eukaryotic", "probability": 0.93701171875}, {"start": 312.84, "end": 313.48, "word": " أقل", "probability": 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"probability": 0.8995361328125}, {"start": 328.25, "end": 328.65, "word": " أكيد", "probability": 0.8221028645833334}, {"start": 328.65, "end": 329.13, "word": " فرصة", "probability": 0.9976806640625}, {"start": 329.13, "end": 329.51, "word": " حدوث", "probability": 0.9825439453125}, {"start": 329.51, "end": 330.21, "word": " الطفرات", "probability": 0.9805908203125}, {"start": 330.21, "end": 330.47, "word": " في", "probability": 0.9873046875}, {"start": 330.47, "end": 330.73, "word": " الـ", "probability": 0.86572265625}, {"start": 330.73, "end": 331.49, "word": " Eukaryotic", "probability": 0.9306640625}, {"start": 331.49, "end": 332.43, "word": " أقل", "probability": 0.9749348958333334}, {"start": 332.43, "end": 333.09, "word": " هذا", "probability": 0.92333984375}, {"start": 333.09, "end": 333.49, "word": " وجه", "probability": 0.93017578125}, {"start": 333.49, "end": 334.45, "word": " المقارنة", "probability": 0.9818359375}, {"start": 334.45, "end": 335.57, "word": " التالت", 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{"start": 357.37, "end": 357.91, "word": " بتكون", "probability": 0.846923828125}, {"start": 357.91, "end": 358.27, "word": " من", "probability": 0.97705078125}, {"start": 358.27, "end": 359.07, "word": " قطعتين", "probability": 0.8870849609375}, {"start": 359.07, "end": 359.87, "word": " قطعة", "probability": 0.9313151041666666}, {"start": 359.87, "end": 360.43, "word": " صغيرة", "probability": 0.99267578125}, {"start": 360.43, "end": 360.65, "word": " و", "probability": 0.98388671875}, {"start": 360.65, "end": 361.03, "word": " قطعة", "probability": 0.8909505208333334}, {"start": 361.03, "end": 361.53, "word": " كبيرة", "probability": 0.97021484375}, {"start": 361.53, "end": 362.85, "word": " الاختلاف", "probability": 0.866943359375}, {"start": 362.85, "end": 363.31, "word": " يكون", "probability": 0.6552734375}, {"start": 363.31, "end": 363.53, "word": " في", "probability": 0.92041015625}, {"start": 363.53, "end": 364.01, "word": " سرعه", "probability": 0.8084716796875}, {"start": 364.01, "end": 364.63, "word": " الترسيب", "probability": 0.885546875}, {"start": 364.63, "end": 364.89, "word": " كل", "probability": 0.9208984375}, {"start": 364.89, "end": 365.13, "word": " من", "probability": 0.95751953125}, {"start": 365.13, "end": 365.41, "word": " هذه", "probability": 0.814453125}, {"start": 365.41, "end": 366.15, "word": " القطعتين", "probability": 0.9892578125}, {"start": 366.15, "end": 366.31, "word": " و", "probability": 0.7734375}, {"start": 366.31, "end": 366.69, "word": " أيضا", "probability": 0.8230794270833334}, {"start": 366.69, "end": 367.09, "word": " سرعه", "probability": 0.9647216796875}, {"start": 367.09, "end": 367.71, "word": " الترسيب", "probability": 0.95244140625}, {"start": 367.71, "end": 368.85, "word": " الريبوسوم", "probability": 0.96435546875}, {"start": 368.85, "end": 369.71, "word": " بقولنا", "probability": 0.7433268229166666}, {"start": 369.71, "end": 369.99, "word": " في", "probability": 0.822265625}, {"start": 369.99, "end": 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"temperature": 1.0}, {"id": 22, "seek": 58533, "start": 555.91, "end": 585.33, "text": "وبيكونوا typical عن الخلية الأصل طيب بالنسبة للبكتيريا كتير ناس لما تسمع كلمة بكتيريا أول ما بخطر ببالها ان إشي هي dark لكن الحقيقة مش هيك الميكروبات بشكل عام ممكن تكون pathogenic وممكن تكون non pathogenic ال non pathogenic يعني غير ممرضة وهذا معظم الميكروبات بتتصنف منها تعتبر non pathogenic", "tokens": [2407, 21292, 30544, 14407, 7476, 18871, 33962, 1211, 10632, 16247, 36520, 23032, 1829, 3555, 20666, 1863, 35457, 3660, 24976, 3555, 4117, 2655, 13546, 25528, 9122, 2655, 13546, 8717, 32277, 5296, 15042, 6055, 38251, 3615, 9122, 19528, 3660, 4724, 4117, 2655, 13546, 25528, 5551, 12610, 19446, 4724, 9778, 9566, 2288, 4724, 3555, 6027, 11296, 16472, 11933, 8592, 1829, 39896, 2877, 44381, 21542, 38436, 28671, 37893, 39896, 4117, 9673, 1829, 4117, 32887, 3555, 9307, 4724, 8592, 28820, 6225, 10943, 3714, 43020, 6055, 30544, 3100, 25473, 4032, 2304, 43020, 6055, 30544, 2107, 3100, 25473, 2423, 2107, 3100, 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"end": 991.03, "word": " host", "probability": 0.58984375}, {"start": 991.03, "end": 992.03, "word": " يبقى", "probability": 0.978515625}, {"start": 992.03, "end": 992.43, "word": " بنحكي", "probability": 0.8846435546875}, {"start": 992.43, "end": 992.59, "word": " الـ", "probability": 0.55511474609375}, {"start": 992.59, "end": 993.21, "word": " Endotoxin", "probability": 0.80841064453125}, {"start": 993.21, "end": 993.89, "word": " تفرزه", "probability": 0.91181640625}, {"start": 993.89, "end": 994.75, "word": " البكتيريا", "probability": 0.98466796875}, {"start": 994.75, "end": 994.95, "word": " و", "probability": 0.755859375}, {"start": 994.95, "end": 995.31, "word": " هي", "probability": 0.8291015625}, {"start": 995.31, "end": 996.13, "word": " ميتة", "probability": 0.9293619791666666}, {"start": 996.13, "end": 996.67, "word": " from", "probability": 0.6650390625}, {"start": 996.67, "end": 997.17, "word": " dead", "probability": 0.9189453125}, {"start": 997.17, "end": 997.89, "word": " bacteria", "probability": 0.93212890625}, {"start": 997.89, "end": 998.87, "word": " أما", "probability": 0.936767578125}, {"start": 998.87, "end": 999.13, "word": " ال", "probability": 0.84814453125}, {"start": 999.13, "end": 999.91, "word": " Exotoxin", "probability": 0.7755126953125}, {"start": 999.91, "end": 1000.59, "word": " خارجي", "probability": 0.9833984375}, {"start": 1000.59, "end": 1001.73, "word": " بتفرزه", "probability": 0.96513671875}, {"start": 1001.73, "end": 1002.65, "word": " البكتيريا", "probability": 0.98681640625}, {"start": 1002.65, "end": 1003.21, "word": " و", "probability": 0.880859375}, {"start": 1003.21, "end": 1003.59, "word": " هي", "probability": 0.9677734375}, {"start": 1003.59, "end": 1004.11, "word": " حية", "probability": 0.83984375}, {"start": 1004.11, "end": 1004.59, "word": " from", "probability": 0.92578125}, {"start": 1004.59, "end": 1005.11, "word": " living", "probability": 0.88671875}, {"start": 1005.11, "end": 1005.75, "word": " bacteria", "probability": 0.943359375}], "temperature": 1.0}, {"id": 39, "seek": 102872, "start": 1006.1, "end": 1028.72, "text": "يبقى ال endotoxin from dead bacteria لكن ال exotoxin from living bacteria عشان هيك لو إنسان مصاب ببكتيريا بتفرز ال endotoxin الدكتور في هذه الحالة مابيعطيه antibiotic بيقتل اللي هو البكتيريا لأنه لو أعطاه antibiotic بيقتل البكتيريا", "tokens": [1829, 3555, 4587, 7578, 2423, 917, 310, 5230, 259, 490, 3116, 11763, 44381, 2423, 454, 310, 5230, 259, 490, 2647, 11763, 6225, 8592, 7649, 39896, 4117, 45164, 36145, 3794, 7649, 3714, 9381, 16758, 4724, 3555, 4117, 2655, 13546, 25528, 39894, 5172, 2288, 11622, 2423, 917, 310, 5230, 259, 32748, 4117, 2655, 13063, 8978, 29538, 21542, 6027, 3660, 19446, 21292, 3615, 9566, 1829, 3224, 37828, 4724, 1829, 38149, 1211, 13672, 1829, 31439, 29739, 4117, 2655, 13546, 25528, 5296, 33456, 3224, 45164, 5551, 3615, 9566, 40294, 37828, 4724, 1829, 38149, 1211, 29739, 4117, 2655, 13546, 25528], "avg_logprob": -0.13799342764051337, "compression_ratio": 1.7653061224489797, "no_speech_prob": 1.0728836059570312e-06, "words": [{"start": 1006.1, "end": 1006.5, "word": "يبقى", "probability": 0.919677734375}, {"start": 1006.5, "end": 1006.58, "word": " ال", "probability": 0.9052734375}, {"start": 1006.58, "end": 1007.64, "word": " endotoxin", "probability": 0.709320068359375}, {"start": 1007.64, "end": 1007.96, "word": " from", "probability": 0.6484375}, {"start": 1007.96, "end": 1008.42, "word": " dead", "probability": 0.86962890625}, {"start": 1008.42, "end": 1009.08, "word": " bacteria", "probability": 0.89599609375}, {"start": 1009.08, "end": 1009.72, "word": " لكن", "probability": 0.810546875}, {"start": 1009.72, "end": 1009.96, "word": " ال", "probability": 0.916015625}, {"start": 1009.96, "end": 1010.78, "word": " exotoxin", "probability": 0.7735595703125}, {"start": 1010.78, "end": 1011.24, "word": " from", "probability": 0.849609375}, {"start": 1011.24, "end": 1011.76, "word": " living", "probability": 0.8642578125}, {"start": 1011.76, "end": 1012.46, "word": " bacteria", "probability": 0.94091796875}, {"start": 1012.46, "end": 1013.26, "word": " عشان", "probability": 0.9396158854166666}, {"start": 1013.26, "end": 1013.7, "word": " هيك", "probability": 0.762451171875}, {"start": 1013.7, "end": 1013.94, "word": " لو", "probability": 0.9072265625}, {"start": 1013.94, "end": 1014.94, "word": " إنسان", "probability": 0.84765625}, {"start": 1014.94, "end": 1015.48, "word": " مصاب", "probability": 0.9739583333333334}, {"start": 1015.48, "end": 1016.4, "word": " ببكتيريا", "probability": 0.9750162760416666}, {"start": 1016.4, "end": 1017.78, "word": " بتفرز", "probability": 0.8955078125}, {"start": 1017.78, "end": 1018.02, "word": " ال", "probability": 0.97216796875}, {"start": 1018.02, "end": 1018.9, "word": " endotoxin", "probability": 0.923828125}, {"start": 1018.9, "end": 1020.04, "word": " الدكتور", "probability": 0.9736328125}, {"start": 1020.04, "end": 1020.24, "word": " في", "probability": 0.97119140625}, {"start": 1020.24, "end": 1020.48, "word": " هذه", "probability": 0.7001953125}, {"start": 1020.48, "end": 1021.2, "word": " الحالة", "probability": 0.9850260416666666}, {"start": 1021.2, "end": 1022.34, "word": " مابيعطيه", "probability": 0.8376057942708334}, {"start": 1022.34, "end": 1023.0, "word": " antibiotic", "probability": 0.8603515625}, {"start": 1023.0, "end": 1024.44, "word": " بيقتل", "probability": 0.92626953125}, {"start": 1024.44, "end": 1024.86, "word": " اللي", "probability": 0.9345703125}, {"start": 1024.86, "end": 1025.16, "word": " هو", "probability": 0.98828125}, {"start": 1025.16, "end": 1025.92, "word": " البكتيريا", "probability": 0.93662109375}, {"start": 1025.92, "end": 1026.32, "word": " لأنه", "probability": 0.8662109375}, {"start": 1026.32, "end": 1026.46, "word": " لو", "probability": 0.96923828125}, {"start": 1026.46, "end": 1026.9, "word": " أعطاه", "probability": 0.9049072265625}, {"start": 1026.9, "end": 1027.4, "word": " antibiotic", "probability": 0.90380859375}, {"start": 1027.4, "end": 1028.0, "word": " بيقتل", "probability": 0.9754638671875}, {"start": 1028.0, "end": 1028.72, "word": " البكتيريا", "probability": 0.987890625}], "temperature": 1.0}, {"id": 40, "seek": 104021, "start": 1030.07, "end": 1040.21, "text": "هيتفرز الـ bacterial endotoxin ويتدمر الخلايا ويتأثر على الـ host هذا بالنسبة لهذا الـ slide", "tokens": [3224, 1829, 2655, 5172, 2288, 11622, 2423, 39184, 35632, 917, 310, 5230, 259, 4032, 36081, 40448, 2288, 33962, 15040, 25528, 4032, 36081, 10721, 49115, 15844, 2423, 39184, 3975, 23758, 20666, 1863, 35457, 3660, 46740, 15730, 2423, 39184, 4137], "avg_logprob": -0.21714743513327378, "compression_ratio": 1.2222222222222223, "no_speech_prob": 5.960464477539063e-08, "words": [{"start": 1030.07, "end": 1030.87, "word": "هيتفرز", "probability": 0.7607828776041666}, {"start": 1030.87, "end": 1031.23, "word": " الـ", "probability": 0.659423828125}, {"start": 1031.23, "end": 1031.61, "word": " bacterial", "probability": 0.58056640625}, {"start": 1031.61, "end": 1032.51, "word": " endotoxin", "probability": 0.9375}, {"start": 1032.51, "end": 1033.31, "word": " ويتدمر", "probability": 0.8155517578125}, {"start": 1033.31, "end": 1033.97, "word": " الخلايا", "probability": 0.9571940104166666}, {"start": 1033.97, "end": 1035.09, "word": " ويتأثر", "probability": 0.9393310546875}, {"start": 1035.09, "end": 1035.45, "word": " على", "probability": 0.861328125}, {"start": 1035.45, "end": 1035.81, "word": " الـ", "probability": 0.81982421875}, {"start": 1035.81, "end": 1036.17, "word": " host", "probability": 0.7587890625}, {"start": 1036.17, "end": 1037.29, "word": " هذا", "probability": 0.9052734375}, {"start": 1037.29, "end": 1038.01, "word": " بالنسبة", "probability": 0.9813232421875}, {"start": 1038.01, "end": 1039.75, "word": " لهذا", "probability": 0.5479736328125}, {"start": 1039.75, "end": 1039.85, "word": " الـ", "probability": 0.5704345703125}, {"start": 1039.85, "end": 1040.21, "word": " slide", "probability": 0.88134765625}], "temperature": 1.0}], "language": "ar", "language_probability": 1.0, "duration": 1041.9258125, "duration_after_vad": 1027.2706250000008}
\ No newline at end of file
diff --git a/PL9fwy3NUQKwZWgAb_V1ATlRRsH-6i3cPC/IO73Edx7DW0_raw.srt b/PL9fwy3NUQKwZWgAb_V1ATlRRsH-6i3cPC/IO73Edx7DW0_raw.srt
new file mode 100644
index 0000000000000000000000000000000000000000..58199d53887be8f97bdfe8bb294b663905d63749
--- /dev/null
+++ b/PL9fwy3NUQKwZWgAb_V1ATlRRsH-6i3cPC/IO73Edx7DW0_raw.srt
@@ -0,0 +1,892 @@
+1
+00:00:00,520 --> 00:00:03,960
+السلام عليكم ورحمة الله وبركاته يعطيكم العافية
+
+2
+00:00:03,960 --> 00:00:06,760
+جميعا في هذه المحاضرة ان شاء الله هنبدأ أول معمل
+
+3
+00:00:06,760 --> 00:00:10,840
+من مساق الـ General Microbiology Laboratory سابقا
+
+4
+00:00:10,840 --> 00:00:15,420
+عرفنا الـ General Microbiology it is the study of
+
+5
+00:00:15,420 --> 00:00:19,400
+microorganism هو العلم الذي يدرس الكائنات الدقيقة
+
+6
+00:00:19,400 --> 00:00:23,000
+ال micro organism اللي بندرسها في علم ال micro
+
+7
+00:00:23,000 --> 00:00:26,560
+biology it is small living organism that cannot be
+
+8
+00:00:26,560 --> 00:00:31,440
+seen by naked eye هي كائنات دقيقةحية لا ترى بالعين
+
+9
+00:00:31,440 --> 00:00:35,300
+المجردة تكلمنا أيضًا عن أقسام علم الـ Microbiology
+
+10
+00:00:35,300 --> 00:00:38,900
+وكان من ضمنها الـ Medical Microbiology المجال اللي
+
+11
+00:00:38,900 --> 00:00:43,560
+بنركز فيه دراستنا عليه كعملين في المجال الطبي it
+
+12
+00:00:43,560 --> 00:00:46,140
+is the study of microbes and they are associated
+
+13
+00:00:46,140 --> 00:00:50,280
+with host هو العلم اللي بيدرس الميكروبات و علاقتها
+
+14
+00:00:50,280 --> 00:00:55,220
+بالعائل ال host ال host اللي نقصد فيه الإنسان هل
+
+15
+00:00:55,220 --> 00:01:00,320
+هذا ال microbe بيسبب مرض لل hostهل الميكروب
+
+16
+00:01:00,320 --> 00:01:06,360
+harmless ما بدر هذا العائل أو هل الميكروب بيكون
+
+17
+00:01:06,360 --> 00:01:10,800
+مفيد للعائل beneficial كل هذا هنتعرف عليه الهدف من
+
+18
+00:01:10,800 --> 00:01:14,660
+مجلس البراتوري هو أن يتواجه الطلاب إلى مختلفة
+
+19
+00:01:14,660 --> 00:01:19,920
+الحياة في عالم الميكروبيا في هدفي من معمل الـ
+
+20
+00:01:19,920 --> 00:01:22,920
+microbiology أنه يكون عندي خلفية عن علم الأحياء
+
+21
+00:01:22,920 --> 00:01:26,800
+الدقيقة نتعرف على الميكروبات أكترفي المحاضرة
+
+22
+00:01:26,800 --> 00:01:31,040
+التعريفية حكينا إنه هدفي من معمل الـ Microbiology
+
+23
+00:01:31,040 --> 00:01:35,440
+إنه نعزل بكتيريا من عينات مرضية ونشخصها عبر فحوصات
+
+24
+00:01:35,440 --> 00:01:39,820
+مختلفة الجملتان نفس الشئ لأن حتى إن أنا أقدر أعزل
+
+25
+00:01:39,820 --> 00:01:43,420
+بكتيريا من عينات مرضية و أشخصها أكيد لازم يكون
+
+26
+00:01:43,420 --> 00:01:47,480
+عندي خلفية عن علم الأحياء الدقيقة أعرف خصائص كل
+
+27
+00:01:47,480 --> 00:01:51,850
+ميكروبأعرف الظروف اللي بينما فيها أعرف الأمراض
+
+28
+00:01:51,850 --> 00:01:56,710
+اللي بيسببها كل micro كل المعلومات اللي بتخص ال
+
+29
+00:01:56,710 --> 00:02:00,150
+micro بقى although the study of microbiology
+
+30
+00:02:00,150 --> 00:02:04,250
+include bacteria, virus, algae and protozoa this
+
+31
+00:02:04,250 --> 00:02:07,450
+lab will concentrate primarily on the bacteria
+
+32
+00:02:07,450 --> 00:02:12,230
+حكينا في علم ال medical microbiology هو علم واسع
+
+33
+00:02:12,230 --> 00:02:17,420
+بيدرس الكائنات الدقيقة منبكتيريا من فيروسات من
+
+34
+00:02:17,420 --> 00:02:22,920
+الجي طحالب and البروتوزوه البروتوزوه هي الأوليات
+
+35
+00:02:22,920 --> 00:02:28,440
+لكن قلنا في هذا المعمل ال essential medical
+
+36
+00:02:28,440 --> 00:02:32,800
+microbiology هنركز على البكتيريا طيب شو هي
+
+37
+00:02:32,800 --> 00:02:37,660
+البروتوزوه البروتوزوه معناها بالعربي هي الأوليات
+
+38
+00:02:37,660 --> 00:02:42,220
+طيب شو بتفرق عن البكتيريابقولنا الـ Protozoa هي
+
+39
+00:02:42,220 --> 00:02:47,600
+unicellular microorganism كائنات وحيدة الخلية
+
+40
+00:02:47,600 --> 00:02:52,480
+Eukaryotic شو يعني Eukaryotic يعني حقيقية النوع
+
+41
+00:02:52,480 --> 00:02:56,500
+طيب شو عكس ال Eukaryotic عكس ال Eukaryotic ال
+
+42
+00:02:56,500 --> 00:03:01,420
+Prokaryotic طيب شو هي البكتيريا البكتيريا هي عبارة
+
+43
+00:03:01,420 --> 00:03:06,240
+عن unicellular microorganism كائنات وحيدة الخلية
+
+44
+00:03:06,240 --> 00:03:11,210
+زي ال Protozoaلكن بتختلف عن البروتوزوه انها
+
+45
+00:03:11,210 --> 00:03:17,370
+بروكاريوتك بداية انه يبقى البروتوزوه unicellular
+
+46
+00:03:17,370 --> 00:03:22,690
+eukaryotic microorganism البكتيريا unicellular
+
+47
+00:03:22,690 --> 00:03:27,310
+بروكاريوتك microorganism بتشبه انهم unicellular
+
+48
+00:03:27,310 --> 00:03:32,710
+لكن بختلفوا ان البروتوزوه eukaryotic و البكتيريا
+
+49
+00:03:32,710 --> 00:03:37,940
+بروكاريوتكخلّينا نعرف شو الفرق بين الـ Eukaryotic
+
+50
+00:03:37,940 --> 00:03:43,240
+و البروكاريوتك البروكاريوتك طبعا هي بداية النواء
+
+51
+00:03:43,240 --> 00:03:48,080
+زي البكتيريا ال Eukaryotic هي حقيقية النواء زي
+
+52
+00:03:48,080 --> 00:03:53,640
+البروتوزوة زي مثلا الإنسان إحنا نعتبر Eukaryotic
+
+53
+00:03:53,640 --> 00:03:59,200
+لو بدنا نعمل مقارنة خلّينا أول وجه مقارنة نتكلم
+
+54
+00:03:59,200 --> 00:04:04,010
+على number of chromosomes عدد الكروموسوماتفي الـ
+
+55
+00:04:04,010 --> 00:04:08,650
+Eukaryotic احنا الإنسان عدد الكرموسومات 46 كرموسوم
+
+56
+00:04:08,650 --> 00:04:13,850
+لكن الـ Prokaryotic زي البكتيريا تتكون من one
+
+57
+00:04:13,850 --> 00:04:19,410
+كرموسوم يبقى أول وجه مقارنة بالنسبة ل number of
+
+58
+00:04:19,410 --> 00:04:24,350
+chromosomes طيب shape of chromosome لو قارننا شكل
+
+59
+00:04:24,350 --> 00:04:28,970
+الكرموسوم بين الـ Prokaryotic و الـ Eukaryotic الـ
+
+60
+00:04:28,970 --> 00:04:33,940
+Eukaryotic الإنسان يعتبر شكل الكرموسوم linearلكن
+
+61
+00:04:33,940 --> 00:04:39,300
+الـ Prokaryotic يُعتبر Circular هاي تاني وجه
+
+62
+00:04:39,300 --> 00:04:43,800
+مقارنة الـ shape of a chromosome الـ Prokaryotic
+
+63
+00:04:43,800 --> 00:04:48,620
+يُعتبر Circular لكن الـ Eukaryotic يُعتبر Linear
+
+64
+00:04:48,620 --> 00:04:55,340
+لو حكينا عن ال mutation rate، معدل حدوث الطفرات
+
+65
+00:04:55,340 --> 00:04:59,840
+الـ Prokaryotic بقولك إن معدل حدوث الطفرات فيها
+
+66
+00:04:59,840 --> 00:05:05,740
+تُعتبر high rateلكن الـ Eukaryotic تعتبر low rate
+
+67
+00:05:05,740 --> 00:05:09,980
+طيب لو اتكلمنا عن السبب اللي بيخلي ان معدل حدوث
+
+68
+00:05:09,980 --> 00:05:14,900
+الطفرات في الـ Eukaryotic أقل من الـ Prokaryotic
+
+69
+00:05:15,630 --> 00:05:19,410
+الـ Eukaryotic حقيقية انه هو زي الإنسان احنا بنعرف
+
+70
+00:05:19,410 --> 00:05:23,550
+في كل مرحلة من مراحل ال cell ال cell cycle بيصير
+
+71
+00:05:23,550 --> 00:05:26,950
+في يدي تصحيح لأي طفرة لأنه في يدي checkpoint
+
+72
+00:05:26,950 --> 00:05:31,490
+بالتالي أكيد فرصة حدوث الطفرات في الـ Eukaryotic
+
+73
+00:05:31,490 --> 00:05:39,010
+أقل هذا وجه المقارنة التالت لـ mutation rate طبعا
+
+74
+00:05:39,010 --> 00:05:45,050
+من أوجه المقارنة المهمة أيضاحتى نفرق بين الـ
+
+75
+00:05:45,050 --> 00:05:49,410
+Prokaryotic و Eukaryotic الريبوسوم الريبوسوم هو
+
+76
+00:05:49,410 --> 00:05:54,530
+مصنع البروتين في الخلية سواء كان الـ Prokaryotic
+
+77
+00:05:54,530 --> 00:05:59,870
+أو الـ Eukaryotic الريبوسوم بتكون من قطعتين قطعة
+
+78
+00:05:59,870 --> 00:06:04,630
+صغيرة و قطعة كبيرة الاختلاف يكون في سرعه الترسيب
+
+79
+00:06:04,630 --> 00:06:08,850
+كل من هذه القطعتين و أيضا سرعه الترسيب الريبوسوم
+
+80
+00:06:08,850 --> 00:06:13,630
+بقولنا في الـ Prokaryoticلما جبنا الـ ribosome و
+
+81
+00:06:13,630 --> 00:06:17,950
+روحنا رسّبنا في جهاز الـ centrifuge لقوا سرعة
+
+82
+00:06:17,950 --> 00:06:24,070
+الترسيب 70 أس لكن لما فصلنا القطعة الكبيرة لحال و
+
+83
+00:06:24,070 --> 00:06:27,730
+القطعة الصغيرة لحال القطعة الكبيرة كانت سرعة
+
+84
+00:06:27,730 --> 00:06:33,830
+الترسيب 50 أس و القطعة الصغيرة كانت 30 أس هذا
+
+85
+00:06:33,830 --> 00:06:38,130
+بالنسبة لـ Sedimentation Rate أما بالنسبة لـ
+
+86
+00:06:38,130 --> 00:06:42,280
+Eukaryoticلما رسّبنا الـ ribosome بشكل كامل في
+
+87
+00:06:42,280 --> 00:06:47,680
+جهاز الـ centrifuge لقينا أنه بترسب على سرعة 80S
+
+88
+00:06:47,680 --> 00:06:52,460
+لما فصلنا القطعة الكبيرة عن القطعة الصغيرة القطعة
+
+89
+00:06:52,460 --> 00:06:57,700
+الكبيرة كانت سرعة الترسيب 60S أما القطعة الصغيرة
+
+90
+00:06:57,700 --> 00:07:05,410
+كانت 40S تمامهدول أوجه المقارنة بين الـ
+
+91
+00:07:05,410 --> 00:07:08,670
+Prokaryotic و الـ Eukaryotic طبعا فين دي أوجه
+
+92
+00:07:08,670 --> 00:07:14,110
+مقارنة أخرى لكن هاي أهم أوجه المقارنة احنا قلنا في
+
+93
+00:07:14,110 --> 00:07:18,270
+sedimentation rate ان سرعة الترسيب لبروكاريوتك
+
+94
+00:07:18,270 --> 00:07:23,650
+الريبوسوم بشكل كامل 70 أس طيب إيش هي الأس؟ الأس هي
+
+95
+00:07:23,650 --> 00:07:29,590
+اختصار للـ pseudoband هي عبارة عن وحدة قياس بتقيص
+
+96
+00:07:29,590 --> 00:07:34,610
+ال rate of sedimentation يبقىلو بنعرف الـ Sudberg
+
+97
+00:07:34,610 --> 00:07:39,350
+as the unit measures the rate of sedimentation
+
+98
+00:07:39,350 --> 00:07:44,030
+rather than size هي واحدة بتقيس معدل سرعي الترسيب
+
+99
+00:07:44,030 --> 00:07:49,270
+للبروتين مش حجم البروتين اللي بترسب طب أنا ليش
+
+100
+00:07:49,270 --> 00:07:52,670
+قولت انه مش حجم البروتين اللي ترسب لأنه احنا قلنا
+
+101
+00:07:52,670 --> 00:07:56,030
+لما رسبنا القطعة الكبيرة كانت في الـ Broker New
+
+102
+00:07:56,030 --> 00:08:00,230
+Tech كانت سرعي الترسيب 50 أس لما رسبنا القطعة
+
+103
+00:08:00,230 --> 00:08:04,620
+الصغيرةكانت سرعة الترسيب 30 أس لكن لما رسّبنا
+
+104
+00:08:04,620 --> 00:08:09,580
+الريبوسومب كامل كان 70 ماكان مجموحهم 80 يبقى احنا
+
+105
+00:08:09,580 --> 00:08:13,440
+ما بنجمع هذه السرعة اللي بترسب عندها ال protein
+
+106
+00:08:13,440 --> 00:08:19,740
+ككل تمام هذا بالنسبة لأوجه المقارنة بين ال
+
+107
+00:08:19,740 --> 00:08:22,320
+biokaryotic و الeokaryotic
+
+108
+00:08:25,080 --> 00:08:29,720
+بكتيريا are unicellular prokaryotic microorganism
+
+109
+00:08:29,720 --> 00:08:34,560
+اتكلمنا ان هي عبارة عن unicellular وحيدة الخلية
+
+110
+00:08:34,560 --> 00:08:39,560
+prokaryotic بداية ان هو micro organism طبعا لو انا
+
+111
+00:08:39,560 --> 00:08:43,440
+غيرته حاجات unicellular ايوكاريوتيك micro organism
+
+112
+00:08:43,440 --> 00:08:49,610
+بتكلم عن proto zoathat divide by binary effusion
+
+113
+00:08:49,610 --> 00:08:54,250
+بتنقسم بواسطة الانشطار الصناعي أو binary division
+
+114
+00:08:54,250 --> 00:09:00,210
+نفس الكلمة هان بعرفلي ال binary effusion a process
+
+115
+00:09:00,210 --> 00:09:04,850
+by which one bacterium split into two ان الخلية
+
+116
+00:09:04,850 --> 00:09:11,070
+البكتيرية الواحدة بتنقسم split to two لأ خليتين
+
+117
+00:09:11,070 --> 00:09:14,730
+بكتيريتين هدول الخليتين البكتيريتين بيكونوا
+
+118
+00:09:14,730 --> 00:09:19,790
+identicalوبيكونوا typical عن الخلية الأصل طيب
+
+119
+00:09:19,790 --> 00:09:24,890
+بالنسبة للبكتيريا كتير ناس لما تسمع كلمة بكتيريا
+
+120
+00:09:24,890 --> 00:09:29,310
+أول ما بخطر ببالها ان إشي هي dark لكن الحقيقة مش
+
+121
+00:09:29,310 --> 00:09:34,430
+هيك الميكروبات بشكل عام ممكن تكون pathogenic وممكن
+
+122
+00:09:34,430 --> 00:09:38,630
+تكون non pathogenic ال non pathogenic يعني غير
+
+123
+00:09:38,630 --> 00:09:44,550
+ممرضة وهذا معظم الميكروبات بتتصنف منها تعتبر non
+
+124
+00:09:44,550 --> 00:09:49,310
+pathogenicلكن ال pathogenic microbe اللي هي اللي
+
+125
+00:09:49,310 --> 00:09:52,790
+ممرضة مايعني pathogenic يعني أنها بتعمل disease
+
+126
+00:09:52,790 --> 00:09:57,570
+نسبة قليلة من ال micro part تعتبر pathogenic يبقى
+
+127
+00:09:57,570 --> 00:10:02,410
+النسبة الأكبر أو المعظم تكون non pathogenic ال non
+
+128
+00:10:02,410 --> 00:10:06,350
+pathogenic ممكن أنها تكون تصنف تحتها harmless إنها
+
+129
+00:10:06,350 --> 00:10:12,210
+ما بتضر وممكن كمان إنها تصنف beneficial إنها إلها
+
+130
+00:10:12,210 --> 00:10:16,830
+فوائدفي عندى برضه تصنيف تالت احنا صنفناها لأ
+
+131
+00:10:16,830 --> 00:10:19,950
+pathogenic وهذا عدد قليل non pathogenic معظم
+
+132
+00:10:19,950 --> 00:10:23,710
+الميكروباط non pathogenic في عندى صنف تالت بقولك
+
+133
+00:10:23,710 --> 00:10:27,070
+pathogenic or non pathogenic يعني هذه البكتيريا
+
+134
+00:10:27,070 --> 00:10:31,330
+ممكن تكون ممرضة في أوقات وممكن تكون غير ممرضة في
+
+135
+00:10:31,330 --> 00:10:36,410
+أوقات أخرى طيب على حسب شو بتكون ممرضة وعلى أي أساس
+
+136
+00:10:36,410 --> 00:10:41,050
+بتكون غير ممرضة بقولنا حسب ال immune system في ال
+
+137
+00:10:41,050 --> 00:10:47,080
+host حسبجهاز المناعة في العقل الـ immune system لو
+
+138
+00:10:47,080 --> 00:10:51,160
+كان الشخص لو كان ال immune system ميح وكان الشخص
+
+139
+00:10:51,160 --> 00:10:55,600
+صليم يعني هذه البكتيريا non pathogenic غير ممرضة
+
+140
+00:10:55,600 --> 00:11:00,780
+لكن لو كان الشخص immunocompromised يعني منعته
+
+141
+00:11:00,780 --> 00:11:06,880
+ضعيفة بتكون pathogenic بتكون ممرضة نرجع لأ الفقرة
+
+142
+00:11:06,880 --> 00:11:12,190
+للذنبof all bacteria cause disease هان بقوللي إنه
+
+143
+00:11:12,190 --> 00:11:16,870
+النسبة أقل من 10% من البكتيريا بتسبب disease
+
+144
+00:11:16,870 --> 00:11:21,370
+واتفقنا عليه إنه ال pathogenic bacteria بتكون نسبة
+
+145
+00:11:21,370 --> 00:11:25,450
+قليلة many others are completely harmless كتير
+
+146
+00:11:25,450 --> 00:11:31,450
+منها تعتبر harmless يعني إنها ما بتأذي واتفقنا إنه
+
+147
+00:11:31,450 --> 00:11:35,780
+البكتيريا النسبة أو النسبة الأكبرإنها non
+
+148
+00:11:35,780 --> 00:11:39,740
+-pathogenic ممكن تكون harmless وممكن تكون أيضا
+
+149
+00:11:39,740 --> 00:11:44,020
+beneficial some bacteria even do good things for
+
+150
+00:11:44,020 --> 00:11:48,780
+us such as Turnbull كيوم صنفليها لتلت أصناف الصنف
+
+151
+00:11:48,780 --> 00:11:54,040
+الأول هي pathogenic وهي نسبة أقل من 10% الصنف
+
+152
+00:11:54,040 --> 00:11:58,360
+التاني قلنا هي non-pathogenic ممكن تكون harmless
+
+153
+00:11:58,360 --> 00:12:03,750
+وممكن تكون beneficialبنسبة للبكتيريا قلنا إنه ممكن
+
+154
+00:12:03,750 --> 00:12:07,450
+تكون صمنها beneficial إلها فوائد على سبيل المثال
+
+155
+00:12:07,450 --> 00:12:11,850
+ذاكر إنه في عيناه بكتيريا بتحول الحليب الـ milk to
+
+156
+00:12:11,850 --> 00:12:17,170
+الجبنة هذه البكتيريا اسمها الـ lactobacillus هتمر
+
+157
+00:12:17,170 --> 00:12:22,710
+معنا بترفع درجة الحموضة البيئة شوي بيتحول الحليب
+
+158
+00:12:22,710 --> 00:12:28,530
+لجبنةمن أيضا في من ضمن البكتيريا اللي إلها فوائد
+
+159
+00:12:28,530 --> 00:12:33,070
+في بكتيريا عندنا بتصنع vitamin K في الأمعاق في
+
+160
+00:12:33,070 --> 00:12:37,970
+بكتيريا عندنا بتخدم القليل في بكتيريا في أمعاق
+
+161
+00:12:37,970 --> 00:12:42,330
+أمعاقنا تعتبر normal flora اسمها normal flora
+
+162
+00:12:42,330 --> 00:12:45,510
+هتمور معاكم بكتيريا موجودة بشكل طبيعي في جسم
+
+163
+00:12:45,510 --> 00:12:49,810
+الإنسان إلها أدوار كبيرة في العمليات الإيدي
+
+164
+00:12:51,000 --> 00:12:55,440
+Bacteria often get a bad reputation as most people
+
+165
+00:12:55,440 --> 00:12:58,840
+tend to associate bacteria with disease بقولنا إن
+
+166
+00:12:58,840 --> 00:13:02,560
+هنا في سمعة سيئة على البكتيريا البكتيريا
+
+167
+00:13:02,560 --> 00:13:09,060
+reputation معناها سمعة هذه السمعة بقولك طبعا سيئة
+
+168
+00:13:09,060 --> 00:13:13,950
+لأ البكتيريا لأن كتير منها بيسببلي أمراضassociated
+
+169
+00:13:13,950 --> 00:13:18,150
+بكتيريا wet disease كتير منها بيسببلي أمراض أكيد
+
+170
+00:13:18,150 --> 00:13:21,930
+هاي السمع غلط لأنه قبل شوية ذكرنا أنه فقط أقل من
+
+171
+00:13:21,930 --> 00:13:27,330
+عشرة بالمية من البكتيريا بيسببلي أمراض بقول إنه
+
+172
+00:13:27,330 --> 00:13:32,110
+كتير من الناس بربطوا البكتيريا بأنها ممرضة لأنه في
+
+173
+00:13:32,110 --> 00:13:36,950
+يوم من الأيام سببت لهم مرض وأيضا في بعض منهم كانت
+
+174
+00:13:36,950 --> 00:13:41,210
+مناعتهم ضعيفة وسببت لهم مرض وكانت abortionist هي
+
+175
+00:13:41,210 --> 00:13:45,600
+because some of themcaused disease لأن بعض من
+
+176
+00:13:45,600 --> 00:13:50,880
+الناس سببت لهم مرض and some other وناس آخرين are
+
+177
+00:13:50,880 --> 00:13:53,800
+abortionistic كانت بالنسبة لإلهم البكتيريا
+
+178
+00:13:53,800 --> 00:13:59,120
+انتهازية انتهازية يعني استغلت ضعف جهاز المناع
+
+179
+00:13:59,120 --> 00:14:05,220
+بقولك البكتيريا الانتهازية هاي فقط that is that is
+
+180
+00:14:05,220 --> 00:14:09,540
+they can cause disease in an ill or injured person
+
+181
+00:14:09,960 --> 00:14:13,640
+هذه البكتيريا الانتهازية بتعمل فقط مرض في الإنسان
+
+182
+00:14:13,640 --> 00:14:17,540
+المريض لما يكون إن الإنسان في عنده ضعف جهاز المناع
+
+183
+00:14:17,540 --> 00:14:21,960
+لما يكون الإنسان مريض هذه الـ abortionistic
+
+184
+00:14:21,960 --> 00:14:26,360
+بكتيريا بتنتهز الفرصة و بتسبب مرض للإنسان many
+
+185
+00:14:26,360 --> 00:14:30,400
+bacteria cannot even live at the temperature found
+
+186
+00:14:30,400 --> 00:14:33,820
+in and on the human body في كتير من البكتيريا ما
+
+187
+00:14:33,820 --> 00:14:38,210
+بتقدر تعيش بدرجة حرارة جسم الإنسانلازم حتى اني انا
+
+188
+00:14:38,210 --> 00:14:43,330
+اعزلها اوفرلها الظروف المناسبة bacteria are
+
+189
+00:14:43,330 --> 00:14:47,070
+everywhere اتكلمنا ان البكتيريا موجودة في كل مكان
+
+190
+00:14:47,070 --> 00:14:51,270
+موجودة على الأسطح موجودة في التربة موجودة على
+
+191
+00:14:51,270 --> 00:14:54,850
+جسمنا on every surface of the body موجودة على كل
+
+192
+00:14:54,850 --> 00:14:58,850
+سطح في جسمنا في وجهنا على أيدينا في كل مكان في
+
+193
+00:14:58,850 --> 00:15:03,190
+جسمنا موجودة البكتيرياincluding digestive tract
+
+194
+00:15:03,190 --> 00:15:06,390
+كذلك موجودة في الـ digestive tract أكيد حتى إنها
+
+195
+00:15:06,390 --> 00:15:11,090
+تساعد في هضم الطعام صنفنا البكتيريا إنها harmless
+
+196
+00:15:11,090 --> 00:15:17,210
+غير مؤذية beneficial طبعا مفيدة هدولة الصنفين
+
+197
+00:15:17,210 --> 00:15:21,470
+يعتبروا non pathogenic غير مضرة وهي نسبة الأكتر
+
+198
+00:15:21,470 --> 00:15:26,290
+pathogenic بكتيريا بتعمل أمراض وهي أقل من 10% من
+
+199
+00:15:26,290 --> 00:15:31,600
+البكتيريابقولك الـ bacteria absorbs nutrients and
+
+200
+00:15:31,600 --> 00:15:34,100
+releases toxins that damage cell and tissue
+
+201
+00:15:34,100 --> 00:15:37,940
+البكتيريا بتنطص المواد الغذائية أكيد بتنطص حتى
+
+202
+00:15:37,940 --> 00:15:41,480
+تقدر تنمو وتتكثر و تكون قادرة على إحداث المرض
+
+203
+00:15:41,480 --> 00:15:46,140
+وأيضا بتفرز توكسين بتفرز السموم السموم تعتبر
+
+204
+00:15:46,140 --> 00:15:49,940
+كviolence factor هنسمع هذه الكلمة الviolence
+
+205
+00:15:49,940 --> 00:15:54,520
+factor معناها عوامل شراسة بتستخدمها البكتيريا حتى
+
+206
+00:15:54,520 --> 00:15:58,870
+إنها تكون قادرة على إحداث المرضبتدمر الخلايا و
+
+207
+00:15:58,870 --> 00:16:03,750
+الانسجار بالنسبة للسموم التوكسين السموم اللى
+
+208
+00:16:03,750 --> 00:16:08,990
+بتفرزها البكتيريا فى عندنا أنواع لهذه التوكسين
+
+209
+00:16:08,990 --> 00:16:14,150
+ممكن تكون اندوتوكسين و ممكن تكون اكسوتوكسين طيب شو
+
+210
+00:16:14,150 --> 00:16:18,990
+الفرق بين الاندوتوكسين و الاكسوتوكسين الاندوتوكسين
+
+211
+00:16:18,990 --> 00:16:24,010
+موجود من اسمه داخل البكتيريا يعنيلو ماتت البكتيريا
+
+212
+00:16:24,010 --> 00:16:28,110
+راح يطلع هذا التوكسين ويأثر على الخلايا و الانسجا
+
+213
+00:16:28,110 --> 00:16:33,210
+ويدمر هذا العائل الـ host يبقى بنحكي الـ Endotoxin
+
+214
+00:16:33,210 --> 00:16:38,870
+تفرزه البكتيريا و هي ميتة from dead bacteria أما
+
+215
+00:16:38,870 --> 00:16:44,590
+ال Exotoxin خارجي بتفرزه البكتيريا و هي حية from
+
+216
+00:16:44,590 --> 00:16:48,420
+living bacteriaيبقى ال endotoxin from dead
+
+217
+00:16:48,420 --> 00:16:53,260
+bacteria لكن ال exotoxin from living bacteria عشان
+
+218
+00:16:53,260 --> 00:16:58,900
+هيك لو إنسان مصاب ببكتيريا بتفرز ال endotoxin
+
+219
+00:16:58,900 --> 00:17:04,440
+الدكتور في هذه الحالة مابيعطيه antibiotic بيقتل
+
+220
+00:17:04,440 --> 00:17:08,000
+اللي هو البكتيريا لأنه لو أعطاه antibiotic بيقتل
+
+221
+00:17:08,000 --> 00:17:13,310
+البكتيرياهيتفرز الـ bacterial endotoxin ويتدمر
+
+222
+00:17:13,310 --> 00:17:19,850
+الخلايا ويتأثر على الـ host هذا بالنسبة لهذا الـ
+
+223
+00:17:19,850 --> 00:17:20,210
+slide
+
diff --git a/PL9fwy3NUQKwZWgAb_V1ATlRRsH-6i3cPC/InTN5cUZCVI.srt b/PL9fwy3NUQKwZWgAb_V1ATlRRsH-6i3cPC/InTN5cUZCVI.srt
new file mode 100644
index 0000000000000000000000000000000000000000..fe63d17655ca4ad5a2f083bb1821db03f9f31475
--- /dev/null
+++ b/PL9fwy3NUQKwZWgAb_V1ATlRRsH-6i3cPC/InTN5cUZCVI.srt
@@ -0,0 +1,1062 @@
+1
+00:00:02,530 --> 00:00:06,650
+السلام عليكم ورحمة الله وبركاته يعطيكم العافية
+
+2
+00:00:06,650 --> 00:00:11,090
+جميعًا في هذا اللقاء سنستكمل موضوع الستاين في
+
+3
+00:00:11,090 --> 00:00:15,470
+المحاضرة السابقة، قسمنا الستاين حسب الـ function إلى
+
+4
+00:00:15,470 --> 00:00:19,770
+simple stain، differential stain، و special stain
+
+5
+00:00:19,770 --> 00:00:23,310
+اليوم إن شاء الله موضوعنا سيكون عن الـ differential
+
+6
+00:00:23,310 --> 00:00:27,550
+stain. اتفقنا أن الـ differential stain هي صبغة
+
+7
+00:00:27,550 --> 00:00:32,140
+تُستخدم لتقسيم البكتيريا إلى مجموعات (to divide bacteria)
+
+8
+00:00:32,140 --> 00:00:36,820
+into large groups. من الأمثلة على الـ Differential
+
+9
+00:00:36,820 --> 00:00:42,860
+stain: جرام stain و Acid Fast stain. بالنسبة لجرام
+
+10
+00:00:42,860 --> 00:00:47,640
+stain، قسمت البكتيريا إلى جرام positive بكتيريا وجرام
+
+11
+00:00:47,640 --> 00:00:51,900
+negative بكتيريا. تم تقسيم البكتيريا في جرام
+
+12
+00:00:51,900 --> 00:00:56,620
+stain على حسب الـ cell wall تبع البكتيريا. طيب، من
+
+13
+00:00:56,620 --> 00:01:01,020
+ماذا تتكون الـ cell wall؟ الـ cell wall تتكون من بيبتيدو
+
+14
+00:01:01,020 --> 00:01:05,600
+غلايكان.  بدنا نتعرف على البيبتيدوغلايكان
+
+15
+00:01:05,600 --> 00:01:12,800
+structure. البيبتيدو
+
+16
+00:01:12,800 --> 00:01:17,160
+غلايكان تتكون من alternative units من وحدات متكررة
+
+17
+00:01:17,160 --> 00:01:20,580
+من الـ N-acetylmuramic acid و الـ
+
+18
+00:01:20,580 --> 00:01:25,040
+N-acetylglucosamine. بتتكرر. بقول لك، لو كان أنا عندي
+
+19
+00:01:25,300 --> 00:01:29,940
+طبقة واحدة من الـ peptidoglycan، تكون البكتيريا
+
+20
+00:01:29,940 --> 00:01:34,520
+جرام negative بكتيريا. لكن لو كان أنا عندي عدة
+
+21
+00:01:34,520 --> 00:01:39,300
+طبقات من الـ peptidoglycan، تكون البكتيريا جرام
+
+22
+00:01:39,300 --> 00:01:44,500
+positive بكتيريا. طيب، عشان يكون عندي عدة طبقات، لازم
+
+23
+00:01:44,500 --> 00:01:48,840
+يكون فيه روابط بين هذه الطبقات. بقول لك إن الـ
+
+24
+00:01:48,840 --> 00:01:53,770
+N-acetylmuramic acid بيطلع منها أربع أحماض أمينية.
+
+25
+00:01:53,770 --> 00:01:57,470
+أربع أحماض أمينية، زي ما إحنا شايفين بالصورة،
+
+26
+00:01:57,470 --> 00:02:05,010
+مربوطين بـ cross-link بـ peptide bond. لو
+
+27
+00:02:05,010 --> 00:02:08,670
+بدي أعمل مقارنة بين الـ Gram positive bacteria و
+
+28
+00:02:08,670 --> 00:02:12,570
+Gram negative bacteria. أول وجه مقارنة: الـ Peptido
+
+29
+00:02:12,570 --> 00:02:15,630
+glycan layer. اتفقنا: لـ Gram positive bacteria
+
+30
+00:02:15,630 --> 00:02:19,600
+Multilayer of Peptidoglycan. لكن الـ Gram-negative
+
+31
+00:02:19,600 --> 00:02:23,380
+bacteria: one layer of peptidoglycan. ثاني
+
+32
+00:02:23,380 --> 00:02:27,160
+شيء: الـ lipid and lipoprotein content. الـ Gram
+
+33
+00:02:27,160 --> 00:02:30,980
+-positive bacteria: low concentration of lipid. لكن
+
+34
+00:02:30,980 --> 00:02:34,120
+الـ Gram-negative bacteria: high concentration، خاصة
+
+35
+00:02:34,120 --> 00:02:37,360
+في الـ outer membrane. الـ outer membrane of the
+
+36
+00:02:37,360 --> 00:02:41,200
+cell wall فقط موجود في الـ Gram-negative bacteria
+
+37
+00:02:41,200 --> 00:02:44,600
+ومش موجود في الـ Gram-positive bacteria. كذلك الـ
+
+38
+00:02:44,600 --> 00:02:47,540
+periplasmic membrane موجود في الـ Gram-negative
+
+39
+00:02:47,540 --> 00:02:50,560
+بكتيريا ومش موجود في الـ Gram-positive بكتيريا.
+
+40
+00:02:50,560 --> 00:02:54,120
+البوليساكاريد (السكريات) في الـ Gram-positive
+
+41
+00:02:54,120 --> 00:02:58,180
+بكتيريا: في عندي بوليساكاريد زي الـ Teichoic acid
+
+42
+00:02:58,180 --> 00:03:01,500
+اللي بيطلع من الـ cell wall، و الـ Lipoteichoic acid
+
+43
+00:03:01,500 --> 00:03:04,760
+اللي بيطلع من الـ cell membrane. على عكس الـ Gram
+
+44
+00:03:04,760 --> 00:03:08,180
+negative بكتيريا: ما عندي بوليساكاريد، لكن في عندي
+
+45
+00:03:08,180 --> 00:03:11,940
+Lipopolysaccharide موجود في الـ outer membrane.
+
+46
+00:03:12,730 --> 00:03:16,610
+الـ Lipopolysaccharide، من الليبيد، اتفقنا أنه لجرام
+
+47
+00:03:16,610 --> 00:03:21,150
+negative بكتيريا: high concentration of lipid، خاصة في
+
+48
+00:03:21,150 --> 00:03:25,990
+الـ outer membrane. آخر وجه مقارنة: التوكسين. لجرام
+
+49
+00:03:25,990 --> 00:03:30,930
+positive بكتيريا بتفرز إكزوتوكسين (heat labile toxin)،
+
+50
+00:03:30,930 --> 00:03:36,130
+يتأثر بدرجة الحرارة، produced from some
+
+51
+00:03:36,130 --> 00:03:40,920
+species of living bacteria (living bacteria). لأن
+
+52
+00:03:40,920 --> 00:03:46,200
+الاندوتوكسين اتفقنا بتفرزه البكتيريا لما تموت (from
+
+53
+00:03:46,200 --> 00:03:51,880
+dead bacteria). بالنسبة للإكزوتوكسين، ممكن تفرزه
+
+54
+00:03:51,880 --> 00:03:55,420
+some species of gram-negative bacteria. لكن
+
+55
+00:03:55,420 --> 00:04:01,480
+الاندوتوكسين هو heat stable toxin produced only by
+
+56
+00:04:01,480 --> 00:04:05,000
+gram-negative bacteria. فقط بتفرزه gram-negative
+
+57
+00:04:05,000 --> 00:04:09,790
+bacteria. لأن الـ endotoxin هو عبارة عن الـ Lipid A
+
+58
+00:04:09,790 --> 00:04:14,010
+اللي موجود في الـ outer membrane، اللي موجود فقط في
+
+59
+00:04:14,010 --> 00:04:17,290
+الـ Gram-negative bacteria. الـ outer membrane
+
+60
+00:04:17,290 --> 00:04:23,270
+بيحتوي على Lipopolysaccharide، اللي بتكون من Core
+
+61
+00:04:23,270 --> 00:04:27,030
+من Lipid A، اللي هو عبارة عن الـ endotoxin، و بتكون
+
+62
+00:04:27,030 --> 00:04:31,370
+أيضًا من الـ O antigen. الـ O antigen هذا بيعمل
+
+63
+00:04:31,370 --> 00:04:37,410
+antigenic variation، بيعمل تنوع. يعني لو دخل ميكروب
+
+64
+00:04:37,410 --> 00:04:41,990
+للجسم، لو دخلت بكتيريا للجسم، بيغير الانتيجين، بالتالي
+
+65
+00:04:41,990 --> 00:04:48,570
+ما بيتعرف عليه جهاز المناعة، البكتيريا.
+
+66
+00:04:48,570 --> 00:04:52,310
+الـ cell wall، بنلاحظ في الصورة، في جرام positive
+
+67
+00:04:52,310 --> 00:04:56,610
+البكتيريا والجرام negative البكتيريا بتحتوي على plasma
+
+68
+00:04:56,610 --> 00:05:01,650
+membrane. لكن الفرق في الـ cell wall: في جرام positive
+
+69
+00:05:01,650 --> 00:05:05,330
+البكتيريا، البيبتيدوغلايكان thick. لكن في الـ
+
+70
+00:05:05,330 --> 00:05:08,750
+Gram-negative، البيبتيدوغلايكان thin. كذلك
+
+71
+00:05:08,750 --> 00:05:11,850
+أيضًا بنلاحظ أنه أنا في عندي في الـ Gram-negative
+
+72
+00:05:11,850 --> 00:05:15,790
+بكتيريا outer membrane of cell wall، مش موجود في
+
+73
+00:05:15,790 --> 00:05:21,170
+الـ Gram-positive بكتيريا. هذه
+
+74
+00:05:21,170 --> 00:05:25,130
+صورة توضيحية للـ Gram-positive بكتيريا. في عندي cell
+
+75
+00:05:25,130 --> 00:05:29,270
+membrane، بيبتيدوغلايكان layer، thick. بالنسبة
+
+76
+00:05:29,270 --> 00:05:32,510
+لـ polysaccharide: في عندي polysaccharide في الـ
+
+77
+00:05:32,510 --> 00:05:36,180
+Gram-positive بكتيريا: teichoic acid موجود في الـ cell
+
+78
+00:05:36,180 --> 00:05:39,940
+wall، و lipoteichoic acid بيطلع من الـ cell membrane.
+
+79
+00:05:39,940 --> 00:05:45,720
+بالنسبة لجرام negative بكتيريا: في cell membrane زي
+
+80
+00:05:45,720 --> 00:05:49,220
+جرام positive بكتيريا. بيبتيدوغلايكان layer thin of
+
+81
+00:05:49,220 --> 00:05:53,380
+cell wall. بتحتوي على outer membrane of cell wall
+
+82
+00:05:53,380 --> 00:05:58,300
+اللي أحد مكوناته الليبوبوليساكاريد، اللي بيتكون
+
+83
+00:05:58,300 --> 00:06:03,300
+من الـ Lipid A، اللي قلنا هو الـ endotoxin. أيضًا في
+
+84
+00:06:03,300 --> 00:06:06,960
+الـ periplasmic space في الـ Gram-negative بكتيريا،
+
+85
+00:06:06,960 --> 00:06:13,200
+مش موجود في الـ Gram-positive بكتيريا. كيف
+
+86
+00:06:13,200 --> 00:06:17,160
+تم اكتشاف الـ Gram stain؟ في طبيب دنماركي اسمه
+
+87
+00:06:17,160 --> 00:06:21,240
+Christian Gram كان بيشتغل بالمختبر، جاب بعض أنواع
+
+88
+00:06:21,240 --> 00:06:25,340
+البكتيريا، جاب بكتيريا X وبكتيريا Y، وصبغها
+
+89
+00:06:25,340 --> 00:06:29,000
+بـ crystal violet. الـ crystal violet هي عبارة عن
+
+90
+00:06:29,000 --> 00:06:33,380
+basic stain، بتحمل شحنة موجبة. هتصبغ البكتيريا سالبة
+
+91
+00:06:33,380 --> 00:06:38,140
+الشحنة. انصبغت كل البكتيريا سواء كانت X أو Y بـ الـ
+
+92
+00:06:38,140 --> 00:06:42,600
+crystal violet، وأخذت اللون الفيوليت. بعد كده أضاف
+
+93
+00:06:42,600 --> 00:06:47,220
+الـ mordant (المثبت)، الـ iodine. ثبت اللون داخل
+
+94
+00:06:47,220 --> 00:06:51,380
+البكتيريا. ثالث خطوة، أضاف الـ decolorizer (مزيل)
+
+95
+00:06:51,380 --> 00:06:56,940
+اللون، مدة معينة، عبارة عن كحول. لاحظ أن اللون راح من
+
+96
+00:06:56,940 --> 00:07:02,000
+البكتيريا Y، لكن بكتيريا X ظلت محتفظة باللون الفيوليت.
+
+97
+00:07:02,000 --> 00:07:06,880
+بعد كده أضاف صبغة ثانية، basic stain، الصفرانين.
+
+98
+00:07:06,880 --> 00:07:10,460
+البكتيريا اللي راح اللون منها أخذت الصبغة.
+
+99
+00:07:10,460 --> 00:07:13,580
+والبكتيريا اللي ما راح اللون منها ما أخذت الصبغة.
+
+100
+00:07:13,580 --> 00:07:17,840
+ظلت محتفظة بالصبغة الأولى، crystal violet. ثم
+
+101
+00:07:17,840 --> 00:07:22,480
+البكتيريا اللي ظلت محتفظة باللون، وما نزل، وأخذت
+
+102
+00:07:22,480 --> 00:07:26,130
+اللون الفيوليت: Gram positive bacteria. واللي أخذت
+
+103
+00:07:26,130 --> 00:07:29,670
+اللون الـ pink وراح اللون منها: Gram-negative
+
+104
+00:07:29,670 --> 00:07:34,510
+bacteria. السؤال: ليش البكتيريا الـ Gram-positive
+
+105
+00:07:34,510 --> 00:07:38,690
+bacteria ظلت محتفظة باللون الـ violet؟ بناءً على
+
+106
+00:07:38,690 --> 00:07:42,770
+المقارنة اللي عملناها، ممكن نفسر هذه النتيجة: الـ
+
+107
+00:07:42,770 --> 00:07:46,230
+Gram-negative bacteria بتتكون من طبقة واحدة من الـ
+
+108
+00:07:46,230 --> 00:07:50,150
+peptidoglycan. بالتالي سهل على الـ decolorizer
+
+109
+00:07:50,150 --> 00:07:54,070
+(الكحول) يدخل ويزيل اللون. لكن الـ Gram-positive
+
+110
+00:07:54,070 --> 00:07:57,330
+بكتيريا بتتكون من multilayer من الـ peptidoglycan.
+
+111
+00:07:57,330 --> 00:08:01,990
+هتظل محتفظة باللون. السبب الثاني: قلنا إنه أنا في
+
+112
+00:08:01,990 --> 00:08:05,030
+عندي في الـ Gram-negative بكتيريا كمية عالية من
+
+113
+00:08:05,030 --> 00:08:09,390
+الدهون (high rich of lipid). و الـ decolorizer عبارة
+
+114
+00:08:09,390 --> 00:08:13,130
+عن كحول، و الكحول يذوب في الدهون، فراح يزيل اللون
+
+115
+00:08:13,130 --> 00:08:18,260
+منها. ولما أصبغ بصبغة ثانية، اللي هي الصفرانين، إيش
+
+116
+00:08:18,260 --> 00:08:21,360
+طبيعي إنها تصبغ البكتيريا اللي مش مصبوغة. لكن
+
+117
+00:08:21,360 --> 00:08:28,560
+البكتيريا المصبوغة هتظل لونها زي ما هو. جرام
+
+118
+00:08:28,560 --> 00:08:32,560
+stain، differential stain. اتفقنا إن الجرام stain
+
+119
+00:08:32,560 --> 00:08:36,380
+هي نوع من أنواع الـ differential stain اللي بتقسم
+
+120
+00:08:36,380 --> 00:08:41,240
+البكتيريا إلى مجموعات. هانز كريستيان جرام، دكتور
+
+121
+00:08:41,240 --> 00:08:44,580
+(he developed a new method to stain bacteria). لذلك
+
+122
+00:08:44,580 --> 00:08:47,720
+يمكن أن يكونوا مشهورين في مجموعات مخصصة. هذه
+
+123
+00:08:47,720 --> 00:08:52,160
+كريستيان جرام، طبيب دنماركي، طور طريقة جديدة
+
+124
+00:08:52,160 --> 00:08:56,720
+لصباغة البكتيريا: الـ Gram stain. قسمت البكتيريا
+
+125
+00:08:56,720 --> 00:09:01,460
+إلى مجموعتين كبيرتين. قسمت البكتيريا إلى مجموعتين: Gram
+
+126
+00:09:01,460 --> 00:09:05,760
+positive و Gram negative. هذا الهدف من Gram
+
+127
+00:09:05,760 --> 00:09:11,400
+stain. Gram status is important in medicine; the
+
+128
+00:09:11,400 --> 00:09:14,960
+presence or absence of a cell wall will change the
+
+129
+00:09:14,960 --> 00:09:19,200
+bacterium’s susceptibility to some antibiotics. بقول لك
+
+130
+00:09:19,200 --> 00:09:23,540
+مهم جدًا أعرف هل البكتيريا جرام positive أو جرام
+
+131
+00:09:23,540 --> 00:09:28,100
+negative. why؟ لأن وجود أو غياب الـ cell wall هيغير
+
+132
+00:09:28,100 --> 00:09:33,550
+استجابة البكتيريا للمضادات الحيوية. كيف؟ المضادات
+
+133
+00:09:33,550 --> 00:09:37,470
+الحيوية بتأثر على البكتيريا من خلال استهدافها
+
+134
+00:09:37,470 --> 00:09:42,670
+لتراكيب داخل الخلية. ممكن تستهدف الـ cell wall، ممكن
+
+135
+00:09:42,670 --> 00:09:46,930
+الـ protein، ممكن الـ ribosome، ممكن أي مكون داخل الخلية.
+
+136
+00:09:46,930 --> 00:09:50,730
+بالنسبة للـ cell wall، لأن الـ Gram stain بنعتمد عليه،
+
+137
+00:09:50,730 --> 00:09:54,550
+قلنا إن الـ cell wall بتكون من بيبتيدوغلايكان.
+
+138
+00:09:54,550 --> 00:09:57,650
+البيبتيدوغلايكان بتكون من وحدات من
+
+139
+00:09:57,650 --> 00:10:01,850
+الـ N-acetylglucosamine و الـ N-acetylmuramic acid.
+
+140
+00:10:02,200 --> 00:10:06,100
+الـ N-acetylmuramic acid بيطلع منها أحماض أمينية.
+
+141
+00:10:06,100 --> 00:10:10,840
+هذه الأحماض مربوطة بـ cross-link بـ peptide bond.
+
+142
+00:10:10,840 --> 00:10:15,740
+في مضادات حيوية زي الـ penicillin بتستهدف هذه
+
+143
+00:10:15,740 --> 00:10:19,600
+الروابط و بتكسرها. يعني بتستهدف فقط الـ Gram
+
+144
+00:10:19,600 --> 00:10:23,040
+positive bacteria. لأن اتفقنا: لـ Gram-positive
+
+145
+00:10:23,040 --> 00:10:26,220
+bacteria: multilayer of peptidoglycan، هي الموجودة
+
+146
+00:10:26,220 --> 00:10:30,000
+فيها الروابط. لكن لـ Gram-negative: طبقة واحدة،
+
+147
+00:10:30,000 --> 00:10:35,610
+ما فيها أي روابط. يعني البنسلين لو أعطيته لمريض مصاب
+
+148
+00:10:35,610 --> 00:10:41,030
+بالتهاب بكتيريا موجبة جرام، هيستجيب لهذا المضاد.
+
+149
+00:10:41,030 --> 00:10:45,090
+لكن لو أعطيته لمريض مصاب بالتهاب بكتيريا سالبة
+
+150
+00:10:45,090 --> 00:10:50,250
+جرام، ما رح يستجيب. يبقى ضروري جدًا أكون عارفة هل
+
+151
+00:10:50,250 --> 00:10:54,990
+البكتيريا موجبة جرام أو سالبة جرام، لأن استجابتها
+
+152
+00:10:54,990 --> 00:10:56,530
+للمضادات هتختلف.
+
+153
+00:11:01,040 --> 00:11:04,680
+Almost all bacteria are described by their Gram
+
+154
+00:11:04,680 --> 00:11:08,600
+stain characteristics. تقريبًا كل البكتيريا بيتم
+
+155
+00:11:08,600 --> 00:11:12,980
+تصنيفها بالـ Gram stain. إذا ملاحظين، ما حكى all
+
+156
+00:11:12,980 --> 00:11:17,200
+bacteria، قال almost all bacteria. ليش؟ لأن في عندي
+
+157
+00:11:17,200 --> 00:11:20,860
+بكتيريا اسمها الـ Mycobacterium. هذه البكتيريا
+
+158
+00:11:20,860 --> 00:11:25,040
+موجودة على الـ cell wall تبعها waxy lipid. الـ waxy
+
+159
+00:11:25,040 --> 00:11:29,780
+lipid بيعمل كحاجز، بمانع دخول الصبغة. فبالتالي ما
+
+160
+00:11:29,780 --> 00:11:33,400
+بقدر أصبغها بالـ simple stain ولا بالـ Gram stain.
+
+161
+00:11:33,400 --> 00:11:38,960
+بلجأ لطريقة أخرى لصبغها: بالـ Acid-fast stain، اللي
+
+162
+00:11:38,960 --> 00:11:43,600
+هنتعرف عليها في هذه المحاضرة. Based on differences
+
+163
+00:11:43,600 --> 00:11:47,780
+in cell wall structure. اتفقنا أن الصبغة بتكون حسب
+
+164
+00:11:47,780 --> 00:11:53,940
+الاختلاف في تركيب الـ cell wall. Reagents
+
+165
+00:11:53,940 --> 00:11:59,010
+for Gram stain: crystal violet (primary stain). هي
+
+166
+00:11:59,010 --> 00:12:02,830
+الصبغة الأولية، أول صبغة بستخدمها. positive stain،
+
+167
+00:12:02,830 --> 00:12:07,150
+يعني بتحمل شحنة موجبة، يعني basic stain، عشان تصبغ
+
+168
+00:12:07,150 --> 00:12:12,170
+البكتيريا سالبة الشحنة.  gives purple color. بتعطي اللون
+
+169
+00:12:12,170 --> 00:12:16,650
+الـ purple. ثاني مكون: الـ iodine. الـ iodine يعتبر
+
+170
+00:12:16,650 --> 00:12:21,290
+mordant (مثبت). و الـ principle تبع الـ iodine بقول
+
+171
+00:12:21,290 --> 00:12:25,070
+ليه؟ combines with primary stain. بيرتبط مع الـ
+
+172
+00:12:25,070 --> 00:12:29,710
+primary stain، اللي هي الـ crystal violet، to form an
+
+173
+00:12:29,710 --> 00:12:34,910
+insoluble complex. عشان يكون insoluble complex. الـ
+
+174
+00:12:34,910 --> 00:12:38,790
+insoluble complex اسمه crystal violet-iodine
+
+175
+00:12:38,790 --> 00:12:4
+
+223
+00:16:14,960 --> 00:16:19,060
+Air-drying to prevent lysis.  الثالث خطوة الـ Heat
+
+224
+00:16:19,060 --> 00:16:23,280
+-fixation to let bacteria stick to the slide and to
+
+225
+00:16:23,280 --> 00:16:27,800
+kill bacteria. الرسمة الموجودة في هذه الصفحة
+
+226
+00:16:27,800 --> 00:16:32,610
+شرحناها نفس ما شرحناها في الصفحة السابقة. أول أشياء
+
+227
+00:16:32,610 --> 00:16:36,710
+أن الخلايا كانت transparent.  قلنا أن البكتيريا
+
+228
+00:16:36,710 --> 00:16:40,810
+من خصائصها أنها transparent (شفافة). صبغتها بـ crystal
+
+229
+00:16:40,810 --> 00:16:43,830
+violet. أخذت اللون سواء أجرام positive أو أجرام
+
+230
+00:16:43,830 --> 00:16:47,650
+negative. ضفت الـ decolorizer (اللي هو الكحول أو
+
+231
+00:16:47,650 --> 00:16:51,010
+الأسيتون). اللون راح من الأجرام negative لكن الأجرام
+
+232
+00:16:51,010 --> 00:16:55,690
+positive ظلت محتفظة باللون violet. ضفت الصفرانين،
+
+233
+00:16:55,690 --> 00:16:59,810
+أخذت اللون البكتيريا التي راح منها اللون، وهي
+
+234
+00:16:59,810 --> 00:17:01,530
+الأجرام negative (البكتيريا).
+
+235
+00:17:07,060 --> 00:17:10,680
+الخطوة الرابعة هي الصباغة وقلنا أنها تختلف حسب نوع
+
+236
+00:17:10,680 --> 00:17:14,100
+الصباغة.  سيمبل ستاين، جرام ستاين،  فاست ستاين
+
+237
+00:17:14,100 --> 00:17:18,300
+بالنسبة لجرام ستاين، أول شيء: application of a
+
+238
+00:17:18,300 --> 00:17:21,700
+crystal violet. بدنا نضيف الـ crystal violet لمدة
+
+239
+00:17:21,700 --> 00:17:27,460
+primary stain لمدة one minute. بعد ما تخلص الدقيقة،
+
+240
+00:17:27,460 --> 00:17:32,260
+هأغسل بالماء. ثاني شيء، هضيف الـ mordant (المثبت)
+
+241
+00:17:32,260 --> 00:17:36,900
+اليود لمدة دقيقة. بعد ما أخلص هذه الدقيقة، سأغسل
+
+242
+00:17:36,900 --> 00:17:41,820
+بالماء. بعدها، هضيف الكحول أو الأسيتون، اللي هو
+
+243
+00:17:41,820 --> 00:17:46,360
+الـ decolorizer (مزيل اللون)، لكن هنا لمدة 15 second.
+
+244
+00:17:46,360 --> 00:17:50,880
+بعد ما أخلص الـ 15 second، هأغسل بالماء. آخر خطوة،
+
+245
+00:17:50,880 --> 00:17:54,340
+سأضيف الـ counterstain أو الـ secondary stain، اللي هي
+
+246
+00:17:54,340 --> 00:17:59,100
+الصفرانين لمدة one minute. بعد ما أخلص، سأغسل بال
+
+247
+00:17:59,100 --> 00:18:03,520
+ماء. ثم جفاف بالهواء (Air Drying). بعدين أشوفها تحت الـ
+
+248
+00:18:03,520 --> 00:18:13,310
+microscope على عدسة 40X و 100X. بالنسبة
+
+249
+00:18:13,310 --> 00:18:17,750
+للنتائج: كيف بدي أكتب نتيجة لـ Gram Stain؟ نتيجة لـ
+
+250
+00:18:17,750 --> 00:18:21,670
+Gram Stain تتكون من شقين. الشق الأول يتعلق بـ: هل هي
+
+251
+00:18:21,670 --> 00:18:25,950
+Gram Positive أو Gram Negative. لو كان اللون Violet،
+
+252
+00:18:25,950 --> 00:18:29,550
+فهي Gram Positive. لكن لو كان اللون Pink أو Red،
+
+253
+00:18:29,550 --> 00:18:33,550
+فهي Gram Negative. الشق الثاني يتعلق بالشكل:
+
+254
+00:18:33,550 --> 00:18:39,070
+هل هي كروية، عصوية، أو حلزونية.  يبقى لو أجينا
+
+255
+00:18:39,070 --> 00:18:42,230
+لهذه الصورة، بدي أكتب نتيجة لـ Gram Stain. سأرى
+
+256
+00:18:42,230 --> 00:18:45,910
+اللون. اللون Red،  إذن هي Gram Negative Bacteria.
+
+257
+00:18:45,910 --> 00:18:50,210
+سأنظر إلى شكل البكتيريا. هي عصوية، إذن النتيجة
+
+258
+00:18:50,210 --> 00:18:54,290
+Gram Negative Bacilli. بدون ما أكتب الـ
+
+259
+00:18:54,290 --> 00:18:58,540
+arrangement. الصورة الثانية، اللون violet، إذن هي
+
+260
+00:18:58,540 --> 00:19:02,480
+جرام positive بكتيريا. الشكل كروي، إذن كوكاي.
+
+261
+00:19:02,480 --> 00:19:11,260
+النتيجة الكاملة: جرام positive كوكاي.  error
+
+262
+00:19:11,260 --> 00:19:15,720
+during staining. never ever use oil culture. أقول
+
+263
+00:19:15,720 --> 00:19:20,280
+ممنوع أستخدم مستعمرات من زراعة قديمة، لأن
+
+264
+00:19:20,280 --> 00:19:24,080
+البكتيريا مع طول المدة، الجدار الخلوي لها يبدأ
+
+265
+00:19:24,080 --> 00:19:28,560
+يتحلل. بالتالي، هي بتكون Multilayer، اتحلل جزء منها،
+
+266
+00:19:28,560 --> 00:19:32,340
+أصبحت مبينة كأنها One Layer. يعني بيعطيني False
+
+267
+00:19:32,340 --> 00:19:35,380
+Result. يعني ممكن البكتيريا تكون في الأصل Gram
+
+268
+00:19:35,380 --> 00:19:38,900
+Positive. بكتيريا، راحت عملتي Gram Stain، أخدت
+
+269
+00:19:38,900 --> 00:19:44,660
+البكتيريا من Old Culture، هتبين في نتيجة Gram Stain
+
+270
+00:19:44,660 --> 00:19:49,290
+كأنها Gram Negative، هتبين لونها لون Pink. بالتالي،
+
+271
+00:19:49,290 --> 00:19:55,550
+ممنوع أن أستخدم بكتيريا في الـ Gram Stain تكون من Old
+
+272
+00:19:55,550 --> 00:19:59,750
+culture. Never ever use a sample from a patient taking
+
+273
+00:19:59,750 --> 00:20:03,130
+antibiotics. اللي ما تأخذي بكتيريا معزولة من مريض
+
+274
+00:20:03,130 --> 00:20:06,590
+بياخذ Antibiotics. أحنا قلنا في أول المحاضرة أن
+
+275
+00:20:06,590 --> 00:20:10,390
+المضادات الحيوية ممكن تستهدف الـ cell wall بالتحديد،
+
+276
+00:20:10,390 --> 00:20:13,830
+بتستهدف الـ cross link، زي الـ penicillin، اللي
+
+277
+00:20:13,830 --> 00:20:18,590
+بيستهدف الـ Gram positive bacteria. يعني لو مريض بياخد
+
+278
+00:20:18,590 --> 00:20:23,750
+penicillin عشان يعالج التهاب بكتيري من البكتيريا
+
+279
+00:20:23,750 --> 00:20:29,790
+الـ Gram positive bacteria، هتبين كأنها في Gram
+
+280
+00:20:29,790 --> 00:20:34,010
+negative bacteria في الجرام ستاين. ليش؟ لأن الـ
+
+281
+00:20:34,010 --> 00:20:36,810
+penicillin بيستهدف الـ cross-link. هي كانت
+
+282
+00:20:36,810 --> 00:20:40,830
+multilayer of peptidoglycan، هتبين كأنها one layer.
+
+283
+00:20:40,830 --> 00:20:45,790
+هيفكك الروابط، هيعمل collapse لـ cell wall. Time of
+
+284
+00:20:45,790 --> 00:20:50,510
+decolorizer. أقول: وقت الـ decolorizer مهم جداً،
+
+285
+00:20:50,510 --> 00:20:55,890
+يُعتبر critical. لو زودت أنا وقت الـ decolorizer،  Gram positive
+
+286
+00:20:55,890 --> 00:21:00,330
+bacteria هتبين كأنها Gram negative bacteria. ليش؟
+
+287
+00:21:00,330 --> 00:21:03,790
+لأنه هيكون في عندي وقت كافي لدخول الـ decolorizer،
+
+288
+00:21:03,790 --> 00:21:07,910
+اللي هو الكحول، إلى الـ multilayer. بالتالي اللون
+
+289
+00:21:07,910 --> 00:21:12,390
+هيروح من الـ Gram positive bacteria.  هتصبح البكتيريا
+
+290
+00:21:12,390 --> 00:21:16,330
+شفافة. فسأصبغ بالصفرانين، ستأخذ اللون pink، وهتبين
+
+291
+00:21:16,330 --> 00:21:21,030
+كأنها Gram negative bacteria. لو أنا قللت وقت الـ
+
+292
+00:21:21,030 --> 00:21:25,350
+decolorizer أقل من 15 second، لـ Gram negative
+
+293
+00:21:25,350 --> 00:21:29,550
+bacteria، هتبين كأنها Gram positive bacteria. ليش؟
+
+294
+00:21:29,550 --> 00:21:33,690
+لأنه مش هيكون في وقت كافي عشان ينزل الـ crystal
+
+295
+00:21:33,690 --> 00:21:37,470
+violet من الـ Gram negative bacteria من الـ peptido
+
+296
+00:21:37,470 --> 00:21:40,930
+glycan. هتبقى الـ Gram Negative محتفظة باللون
+
+297
+00:21:40,930 --> 00:21:44,990
+الـ violet. راحت أنا حطيت الصفرانين، مش هتأخذ الصبغة
+
+298
+00:21:44,990 --> 00:21:49,630
+لأنها ملونة باللون violet. بالتالي الـ Gram
+
+299
+00:21:49,630 --> 00:21:53,690
+Negative هتبين كأنها Gram Positive. Time of
+
+300
+00:21:53,690 --> 00:21:57,850
+fixation. لو أنا زودت وقت الـ fixation أو ضلت حتى الـ
+
+301
+00:21:57,850 --> 00:22:01,190
+slide على اللهب، الـ Gram Positive bacteria هتبين
+
+302
+00:22:01,190 --> 00:22:05,350
+كأنها Gram Negative bacteria. أحنا قلنا الـ fixation
+
+303
+00:22:05,350 --> 00:22:09,930
+بيعمل partial melting of cell wall. لو أنا زودت وقت
+
+304
+00:22:09,930 --> 00:22:13,430
+الـ fixation، هيصير في عندي complete melting للـ cell
+
+305
+00:22:13,430 --> 00:22:19,270
+wall. يبقى هتبين الـ multilayer كأنها one layer.
+
+306
+00:22:19,270 --> 00:22:24,150
+هيصير collapse للـ cell wall.  لا عينة على الشريحة.
+
+307
+00:22:24,150 --> 00:22:28,030
+أحنا قلنا وظيفة الـ fixation عشان تلتصق البكتيريا على
+
+308
+00:22:28,030 --> 00:22:32,110
+الـ slide. بالتالي، لو كان قليل، مش هتلتصق على الـ
+
+309
+00:22:32,110 --> 00:22:36,310
+slide. يبقى مش هيكون فيه عندي عينة. هذه أهم الأخطاء
+
+310
+00:22:36,310 --> 00:22:40,750
+اللي ممكن تحصل خلال عملية Gram Staining. عشان هيك،
+
+311
+00:22:40,750 --> 00:22:45,330
+لازم نلتزم بالوقت في كل خطوة. هيك بنكون خلصنا أول
+
+312
+00:22:45,330 --> 00:22:49,210
+نوع من أنواع الـ Differential Stain، اللي هو Gram
+
+313
+00:22:49,210 --> 00:22:49,810
+Stain
diff --git a/PL9fwy3NUQKwZWgAb_V1ATlRRsH-6i3cPC/InTN5cUZCVI_raw.json b/PL9fwy3NUQKwZWgAb_V1ATlRRsH-6i3cPC/InTN5cUZCVI_raw.json
new file mode 100644
index 0000000000000000000000000000000000000000..7b692c8a8e416c11dfc719d5003500c6c6db7bde
--- /dev/null
+++ b/PL9fwy3NUQKwZWgAb_V1ATlRRsH-6i3cPC/InTN5cUZCVI_raw.json
@@ -0,0 +1 @@
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{"start": 675.04, "end": 675.7, "word": " bacteria", "probability": 0.94482421875}, {"start": 675.7, "end": 676.56, "word": " ليش", "probability": 0.764892578125}, {"start": 676.56, "end": 676.78, "word": " لأن", "probability": 0.758056640625}, {"start": 676.78, "end": 676.98, "word": " في", "probability": 0.92333984375}, {"start": 676.98, "end": 677.2, "word": " عندي", "probability": 0.754638671875}, {"start": 677.2, "end": 677.72, "word": " بكتيريا", "probability": 0.98271484375}, {"start": 677.72, "end": 678.3, "word": " اسمها", "probability": 0.9404296875}, {"start": 678.3, "end": 678.5, "word": " ال", "probability": 0.75341796875}, {"start": 678.5, "end": 678.84, "word": " micro", "probability": 0.353759765625}, {"start": 678.84, "end": 679.7, "word": " bacterium", "probability": 0.87255859375}, {"start": 679.7, "end": 680.26, "word": " هذه", "probability": 0.6943359375}, {"start": 680.26, "end": 680.86, "word": " البكتيريا", "probability": 0.94677734375}, {"start": 680.86, "end": 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أصبغها بالـ Simple Stain ولا بالـ Gram Stain هلجأ لطريقة أخرى لصبغتها بالـ Acid Fast Stain اللي هنتعرف عليها في هذه المحاضرة Based on differences of cell wall structure اتفقنا أن الصبغة بتكون حسب الاختلاف في تركيب ال cell wall Reagent for Gram Stain", "tokens": [21292, 25957, 1211, 9122, 5016, 26108, 11622, 32767, 7649, 3615, 11778, 9778, 12610, 31767, 3555, 17082, 3660, 6156, 3555, 6027, 2655, 6027, 1829, 19446, 4724, 28543, 2288, 5551, 9381, 3555, 17082, 11296, 20666, 39184, 21532, 745, 491, 49429, 20666, 39184, 22130, 745, 491, 8032, 1211, 7435, 10721, 5296, 9566, 16572, 28671, 5551, 34740, 7578, 5296, 9381, 3555, 17082, 2655, 11296, 20666, 39184, 5097, 327, 15968, 745, 491, 13672, 1829, 8032, 29399, 3615, 28480, 25894, 11296, 8978, 29538, 9673, 5016, 46958, 25720, 18785, 322, 7300, 295, 2815, 2929, 3877, 1975, 2655, 5172, 4587, 8315, 14739, 31767, 3555, 17082, 3660, 39894, 30544, 11331, 35457, 2423, 47283, 2655, 15040, 5172, 8978, 6055, 31747, 1829, 3555, 2423, 2815, 2929, 1300, 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"probability": 0.9091796875}, {"start": 704.34, "end": 704.72, "word": " wall", "probability": 0.83740234375}, {"start": 704.72, "end": 705.52, "word": " structure", "probability": 0.82177734375}, {"start": 705.52, "end": 706.14, "word": " اتفقنا", "probability": 0.9248046875}, {"start": 706.14, "end": 706.34, "word": " أن", "probability": 0.450439453125}, {"start": 706.34, "end": 706.92, "word": " الصبغة", "probability": 0.97998046875}, {"start": 706.92, "end": 707.34, "word": " بتكون", "probability": 0.840576171875}, {"start": 707.34, "end": 707.78, "word": " حسب", "probability": 0.981201171875}, {"start": 707.78, "end": 708.56, "word": " الاختلاف", "probability": 0.86240234375}, {"start": 708.56, "end": 708.76, "word": " في", "probability": 0.97216796875}, {"start": 708.76, "end": 709.44, "word": " تركيب", "probability": 0.898681640625}, {"start": 709.44, "end": 709.7, "word": " ال", "probability": 0.8046875}, {"start": 709.7, "end": 709.96, "word": " cell", "probability": 0.487060546875}, {"start": 709.96, "end": 710.52, "word": " wall", "probability": 0.84033203125}, {"start": 710.52, "end": 713.94, "word": " Reagent", "probability": 0.6429443359375}, {"start": 713.94, "end": 714.38, "word": " for", "probability": 0.9453125}, {"start": 714.38, "end": 714.88, "word": " Gram", "probability": 0.52587890625}, {"start": 714.88, "end": 715.42, "word": " Stain", "probability": 0.97998046875}], "temperature": 1.0}, {"id": 27, "seek": 73799, "start": 715.77, "end": 737.99, "text": "Crystal Violet Primer Stain هي الصبغة الأولية أول صبغة بستخدمها Positive Stain يعني بتحمل شح نموجبة يعني Basic Stain عشان تصبغ البكتيريا الصالبة Stain Cellule Parbol بتعطي اللون البربل تاني مكوّن ال Iodine ال Iodine يعتبر Mordant مثبت", "tokens": [38477, 9196, 24383, 302, 2114, 9713, 745, 491, 39896, 31767, 3555, 17082, 3660, 16247, 12610, 10632, 5551, 12610, 20328, 3555, 17082, 3660, 4724, 14851, 9778, 40448, 11296, 46326, 745, 491, 37495, 22653, 39894, 35571, 1211, 13412, 5016, 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iodine complex هذا ال complex بينهجز داخل الcell wall", "tokens": [8592, 2407, 2423, 39184, 8665, 6055, 3555, 3615, 2423, 39184, 286, 378, 533, 4724, 39648, 32239, 3224, 22807, 29520, 365, 6194, 16441, 4724, 13546, 2655, 3555, 9566, 20449, 2423, 6194, 16441, 13672, 1829, 39896, 2423, 13662, 46480, 281, 1254, 364, 1028, 39754, 638, 3997, 6225, 8592, 7649, 7251, 30544, 1028, 39754, 638, 3997, 2423, 1028, 39754, 638, 3997, 24525, 2304, 3224, 13662, 46480, 44422, 533, 3997, 23758, 2423, 3997, 49374, 3224, 7435, 11622, 11778, 47283, 1211, 2423, 4164, 2929], "avg_logprob": -0.24238280691206454, "compression_ratio": 1.7014925373134329, "no_speech_prob": 5.960464477539063e-08, "words": [{"start": 738.45, "end": 738.77, "word": "شو", "probability": 0.517303466796875}, {"start": 738.77, "end": 738.95, "word": " الـ", "probability": 0.800048828125}, {"start": 738.95, "end": 739.45, "word": " principle", "probability": 0.409423828125}, {"start": 739.45, "end": 740.15, "word": " تبع", 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1318.19, "word": " slide", "probability": 0.93359375}, {"start": 1318.19, "end": 1318.43, "word": " على", "probability": 0.8388671875}, {"start": 1318.43, "end": 1318.97, "word": " اللهب", "probability": 0.8943684895833334}, {"start": 1318.97, "end": 1319.39, "word": " ال", "probability": 0.8056640625}, {"start": 1319.39, "end": 1319.69, "word": " Gram", "probability": 0.52685546875}, {"start": 1319.69, "end": 1320.11, "word": " Positive", "probability": 0.93505859375}, {"start": 1320.11, "end": 1320.77, "word": " بكتيريا", "probability": 0.90908203125}, {"start": 1320.77, "end": 1321.19, "word": " هتبين", "probability": 0.98095703125}, {"start": 1321.19, "end": 1321.77, "word": " كإنها", "probability": 0.9798583984375}, {"start": 1321.77, "end": 1322.23, "word": " Gram", "probability": 0.94775390625}, {"start": 1322.23, "end": 1322.63, "word": " Negative", "probability": 0.958984375}, {"start": 1322.63, "end": 1323.29, "word": " بكتيريا", "probability": 0.9900390625}], "temperature": 1.0}, {"id": 49, "seek": 135161, "start": 1323.81, "end": 1351.61, "text": "أحنا قلنا الفكساشن بيعمل partial melting of cell wall لو أنا زودت وقت الفكساشن هيصير في عندي complete melting للcell wall يبقى هتبين ال multilayer كأنها one layer هيصير collapse لل cell wall law no sample on slide أحنا قلنا وظيفة الفكساشن عشان تنتصق البكتيريا على ال slide فبالتالي لو كان قليل مش هتنتصق على", "tokens": [10721, 5016, 8315, 12174, 1211, 8315, 27188, 4117, 3794, 33599, 1863, 4724, 1829, 25957, 1211, 14641, 20493, 295, 2815, 2929, 45164, 41850, 30767, 23328, 2655, 4032, 38149, 27188, 4117, 3794, 33599, 1863, 39896, 9381, 13546, 8978, 18871, 16254, 3566, 20493, 24976, 4164, 2929, 7251, 3555, 4587, 7578, 8032, 2655, 3555, 9957, 2423, 2120, 388, 11167, 9122, 33456, 11296, 472, 4583, 39896, 9381, 13546, 15584, 24976, 2815, 2929, 2101, 572, 6889, 322, 4137, 5551, 5016, 8315, 12174, 1211, 8315, 4032, 19913, 33911, 3660, 27188, 4117, 3794, 33599, 1863, 6225, 8592, 7649, 6055, 1863, 2655, 9381, 4587, 29739, 4117, 2655, 13546, 25528, 15844, 2423, 4137, 6156, 3555, 6027, 2655, 6027, 1829, 45164, 25961, 12174, 20292, 1211, 37893, 8032, 2655, 29399, 9381, 4587, 15844], "avg_logprob": -0.17661372901963407, "compression_ratio": 1.7743190661478598, "no_speech_prob": 7.748603820800781e-07, "words": [{"start": 1323.81, "end": 1324.05, "word": "أحنا", "probability": 0.6505126953125}, {"start": 1324.05, "end": 1324.45, "word": " قلنا", "probability": 0.9098307291666666}, {"start": 1324.45, "end": 1325.35, "word": " الفكساشن", "probability": 0.710302734375}, {"start": 1325.35, "end": 1326.15, "word": " بيعمل", "probability": 0.892333984375}, {"start": 1326.15, "end": 1326.73, "word": " partial", "probability": 0.56689453125}, {"start": 1326.73, "end": 1327.21, "word": " melting", "probability": 0.873046875}, {"start": 1327.21, "end": 1327.57, "word": " of", "probability": 0.94921875}, {"start": 1327.57, "end": 1327.83, "word": " cell", "probability": 0.89013671875}, {"start": 1327.83, "end": 1328.13, "word": " wall", "probability": 0.853515625}, {"start": 1328.13, "end": 1328.73, "word": " لو", "probability": 0.8720703125}, {"start": 1328.73, "end": 1329.03, "word": " أنا", "probability": 0.5791015625}, {"start": 1329.03, "end": 1329.65, "word": " زودت", "probability": 0.9430338541666666}, {"start": 1329.65, "end": 1329.93, "word": " وقت", "probability": 0.9638671875}, {"start": 1329.93, "end": 1330.69, "word": " الفكساشن", "probability": 0.94638671875}, {"start": 1330.69, "end": 1331.09, "word": " هيصير", "probability": 0.7784830729166666}, {"start": 1331.09, "end": 1331.23, "word": " في", "probability": 0.8720703125}, {"start": 1331.23, "end": 1331.45, "word": " عندي", "probability": 0.836181640625}, {"start": 1331.45, "end": 1332.15, "word": " complete", "probability": 0.8486328125}, {"start": 1332.15, "end": 1332.71, "word": " melting", "probability": 0.9150390625}, {"start": 1332.71, "end": 1333.43, "word": " للcell", "probability": 0.5723876953125}, {"start": 1333.43, "end": 1333.77, "word": " wall", "probability": 0.8544921875}, {"start": 1333.77, "end": 1334.51, "word": " يبقى", "probability": 0.972900390625}, {"start": 1334.51, "end": 1335.57, "word": " هتبين", "probability": 0.9571533203125}, {"start": 1335.57, "end": 1336.29, "word": " ال", "probability": 0.95263671875}, {"start": 1336.29, "end": 1337.83, "word": " multilayer", "probability": 0.6668294270833334}, {"start": 1337.83, "end": 1338.33, "word": " كأنها", "probability": 0.85107421875}, {"start": 1338.33, "end": 1338.71, "word": " one", "probability": 0.927734375}, {"start": 1338.71, "end": 1339.27, "word": " layer", "probability": 0.90576171875}, {"start": 1339.27, "end": 1339.67, "word": " هيصير", "probability": 0.9132486979166666}, {"start": 1339.67, "end": 1340.27, "word": " collapse", "probability": 0.5908203125}, {"start": 1340.27, "end": 1340.61, "word": " لل", "probability": 0.8896484375}, {"start": 1340.61, "end": 1340.85, "word": " cell", "probability": 0.51513671875}, {"start": 1340.85, "end": 1341.43, "word": " wall", "probability": 0.88037109375}, {"start": 1341.43, "end": 1342.05, "word": " law", "probability": 0.2373046875}, {"start": 1342.05, "end": 1342.63, "word": " no", "probability": 0.60302734375}, {"start": 1342.63, "end": 1343.13, "word": " sample", "probability": 0.78857421875}, {"start": 1343.13, "end": 1343.55, "word": " on", "probability": 0.9501953125}, {"start": 1343.55, "end": 1344.15, "word": " slide", "probability": 0.947265625}, {"start": 1344.15, "end": 1344.47, "word": " أحنا", "probability": 0.8439127604166666}, {"start": 1344.47, "end": 1344.81, "word": " قلنا", "probability": 0.9700520833333334}, {"start": 1344.81, "end": 1345.37, "word": " وظيفة", "probability": 0.9927978515625}, {"start": 1345.37, "end": 1346.15, "word": " الفكساشن", "probability": 0.95009765625}, {"start": 1346.15, "end": 1346.61, "word": " عشان", "probability": 0.9728190104166666}, {"start": 1346.61, "end": 1347.19, "word": " تنتصق", "probability": 0.8494140625}, {"start": 1347.19, "end": 1347.81, "word": " البكتيريا", "probability": 0.9474609375}, {"start": 1347.81, "end": 1348.03, "word": " على", "probability": 0.794921875}, {"start": 1348.03, "end": 1348.19, "word": " ال", "probability": 0.84716796875}, {"start": 1348.19, "end": 1348.57, "word": " slide", "probability": 0.95947265625}, {"start": 1348.57, "end": 1349.73, "word": " فبالتالي", "probability": 0.98974609375}, {"start": 1349.73, "end": 1349.91, "word": " لو", "probability": 0.9326171875}, {"start": 1349.91, "end": 1350.15, "word": " كان", "probability": 0.99267578125}, {"start": 1350.15, "end": 1350.49, "word": " قليل", "probability": 0.9093424479166666}, {"start": 1350.49, "end": 1350.67, "word": " مش", "probability": 0.9716796875}, {"start": 1350.67, "end": 1351.37, "word": " هتنتصق", "probability": 0.893359375}, {"start": 1351.37, "end": 1351.61, "word": " على", "probability": 0.931640625}], "temperature": 1.0}, {"id": 50, "seek": 136981, "start": 1351.89, "end": 1369.81, "text": "الـ slide يبقى مش هيكون فيه عندي عينة هذه أهم الأخطاء اللي ممكن تحصل خلال عملية Gram Staining عشان هيك لازم نلتزم بالوقت في كل خطوة هيك بنكون خلصنا أول نوع من أنواع الـDifferential Stain اللي هو Gram Stain", "tokens": [6027, 39184, 4137, 7251, 3555, 4587, 7578, 37893, 39896, 30544, 8978, 3224, 18871, 16254, 6225, 9957, 3660, 29538, 5551, 16095, 16247, 9778, 9566, 16606, 13672, 1829, 3714, 43020, 6055, 5016, 36520, 16490, 1211, 6027, 6225, 42213, 10632, 22130, 745, 3686, 6225, 8592, 7649, 39896, 4117, 5296, 31377, 2304, 8717, 1211, 2655, 11622, 2304, 20666, 30543, 2655, 8978, 28242, 16490, 9566, 2407, 3660, 39896, 4117, 44945, 30544, 16490, 1211, 9381, 8315, 5551, 12610, 8717, 45367, 9154, 14739, 14407, 3615, 2423, 39184, 35, 15790, 831, 745, 491, 13672, 1829, 31439, 22130, 745, 491], "avg_logprob": -0.2593410364959551, "compression_ratio": 1.5446009389671362, "no_speech_prob": 1.823902130126953e-05, "words": [{"start": 1351.89, "end": 1352.11, "word": "الـ", "probability": 0.36767578125}, {"start": 1352.11, "end": 1352.53, "word": " slide", "probability": 0.455810546875}, {"start": 1352.53, "end": 1352.81, "word": " يبقى", "probability": 0.8172607421875}, {"start": 1352.81, "end": 1352.93, "word": " مش", "probability": 0.9521484375}, {"start": 1352.93, "end": 1353.31, "word": " هيكون", "probability": 0.92529296875}, {"start": 1353.31, "end": 1353.59, "word": " فيه", "probability": 0.6397705078125}, {"start": 1353.59, "end": 1353.93, "word": " عندي", "probability": 0.61676025390625}, {"start": 1353.93, "end": 1354.77, "word": " عينة", "probability": 0.5613199869791666}, {"start": 1354.77, "end": 1355.23, "word": " هذه", "probability": 0.4111328125}, {"start": 1355.23, "end": 1355.73, "word": " أهم", "probability": 0.968017578125}, {"start": 1355.73, "end": 1356.31, "word": " الأخطاء", "probability": 0.989990234375}, {"start": 1356.31, "end": 1356.47, "word": " اللي", "probability": 0.834228515625}, {"start": 1356.47, "end": 1356.77, "word": " ممكن", "probability": 0.982666015625}, {"start": 1356.77, "end": 1357.27, "word": " تحصل", "probability": 0.6529947916666666}, {"start": 1357.27, "end": 1357.89, "word": " خلال", "probability": 0.8951822916666666}, {"start": 1357.89, "end": 1358.53, "word": " عملية", "probability": 0.94482421875}, {"start": 1358.53, "end": 1359.41, "word": " Gram", "probability": 0.2509765625}, {"start": 1359.41, "end": 1360.13, "word": " Staining", "probability": 0.82568359375}, {"start": 1360.13, "end": 1360.53, "word": " عشان", "probability": 0.8982747395833334}, {"start": 1360.53, "end": 1360.75, "word": " هيك", "probability": 0.556884765625}, {"start": 1360.75, "end": 1361.09, "word": " لازم", "probability": 0.8828125}, {"start": 1361.09, "end": 1362.01, "word": " نلتزم", "probability": 0.9326171875}, {"start": 1362.01, "end": 1362.43, "word": " بالوقت", "probability": 0.781005859375}, {"start": 1362.43, "end": 1362.61, "word": " في", "probability": 0.9130859375}, {"start": 1362.61, "end": 1362.89, "word": " كل", "probability": 0.9873046875}, {"start": 1362.89, "end": 1363.51, "word": " خطوة", "probability": 0.89501953125}, {"start": 1363.51, "end": 1364.09, "word": " هيك", "probability": 0.7159423828125}, {"start": 1364.09, "end": 1364.39, "word": " بنكون", "probability": 0.875}, {"start": 1364.39, "end": 1364.89, "word": " خلصنا", "probability": 0.965087890625}, {"start": 1364.89, "end": 1365.33, "word": " أول", "probability": 0.96435546875}, {"start": 1365.33, "end": 1365.59, "word": " نوع", "probability": 0.96630859375}, {"start": 1365.59, "end": 1365.77, "word": " من", "probability": 0.98193359375}, {"start": 1365.77, "end": 1366.15, "word": " أنواع", "probability": 0.9469401041666666}, {"start": 1366.15, "end": 1366.87, "word": " الـDifferential", "probability": 0.677978515625}, {"start": 1366.87, "end": 1367.45, "word": " Stain", "probability": 0.87841796875}, {"start": 1367.45, "end": 1367.95, "word": " اللي", "probability": 0.7138671875}, {"start": 1367.95, "end": 1368.31, "word": " هو", "probability": 0.97509765625}, {"start": 1368.31, "end": 1369.21, "word": " Gram", "probability": 0.5458984375}, {"start": 1369.21, "end": 1369.81, "word": " Stain", "probability": 0.8984375}], "temperature": 1.0}], "language": "ar", "language_probability": 1.0, "duration": 1371.0454375, "duration_after_vad": 1339.025624999999}
\ No newline at end of file
diff --git a/PL9fwy3NUQKwZWgAb_V1ATlRRsH-6i3cPC/InTN5cUZCVI_raw.srt b/PL9fwy3NUQKwZWgAb_V1ATlRRsH-6i3cPC/InTN5cUZCVI_raw.srt
new file mode 100644
index 0000000000000000000000000000000000000000..a02ea41558b752571c34a137337f08c7d796e1f0
--- /dev/null
+++ b/PL9fwy3NUQKwZWgAb_V1ATlRRsH-6i3cPC/InTN5cUZCVI_raw.srt
@@ -0,0 +1,1252 @@
+1
+00:00:02,530 --> 00:00:06,650
+السلام عليكم ورحمة الله وبركاته يعطيكم العافية
+
+2
+00:00:06,650 --> 00:00:11,090
+جميعا في هذا اللقاء هنستكمل في موضوع الستان في
+
+3
+00:00:11,090 --> 00:00:15,470
+المحاضرة السابقة قسمنا الاستان حسب ال function ل
+
+4
+00:00:15,470 --> 00:00:19,770
+symbol استان differential استان و special استان
+
+5
+00:00:19,770 --> 00:00:23,310
+اليوم ان شاء الله موضوعنا هيكون عن ال differential
+
+6
+00:00:23,310 --> 00:00:27,550
+استان اتفقنا ان ال differential استان هي صبغة
+
+7
+00:00:27,550 --> 00:00:32,140
+تستخدم لتقسيم البكتيريا لمجموعاتDivide bacteria
+
+8
+00:00:32,140 --> 00:00:36,820
+into large groups من الأمثلة على الـDifferential
+
+9
+00:00:36,820 --> 00:00:42,860
+Stain لجرام Stain والAcid Fast Stain بالنسبة لجرام
+
+10
+00:00:42,860 --> 00:00:47,640
+Stain قسمت البكتيريا لجرام Positive بكتيريا وجرام
+
+11
+00:00:47,640 --> 00:00:51,900
+Negative بكتيريا تم تقسيم البكتيريا فيه لجرام
+
+12
+00:00:51,900 --> 00:00:56,620
+Stain على حسب الـcell wall تبع البكتيريا طيب من أش
+
+13
+00:00:56,620 --> 00:01:01,020
+بتكون ال cell wall؟الـ Cell Wall بتكون من بيبتيدو
+
+14
+00:01:01,020 --> 00:01:05,600
+إجلايكان بدنا نتعرف على البيبتيدو إجلايكان
+
+15
+00:01:05,600 --> 00:01:12,800
+structure البيبتيدو
+
+16
+00:01:12,800 --> 00:01:17,160
+إجلايكان بتكون من alternative unit من وحدات متكررة
+
+17
+00:01:17,160 --> 00:01:20,580
+من الـ unacetylamuramic acid و الـ
+
+18
+00:01:20,580 --> 00:01:25,040
+unacetylglucosamine بتتكرر بقولنا لو كان أنا عندي
+
+19
+00:01:25,300 --> 00:01:29,940
+طبقة واحدة من الـ peptidoglycan بتكون البكتيريا
+
+20
+00:01:29,940 --> 00:01:34,520
+إجرام نيجاتيف بكتيريا لكن لو كان أنا عندي عدة
+
+21
+00:01:34,520 --> 00:01:39,300
+طبقات من الـ peptidoglycan بتكون البكتيريا إجرام
+
+22
+00:01:39,300 --> 00:01:44,500
+positive بكتيريا طيب عشان يكون عندي عدة طبقات لازم
+
+23
+00:01:44,500 --> 00:01:48,840
+يكون فيه روابط بين هاي الطبقات بقولنا إن الـ
+
+24
+00:01:48,840 --> 00:01:53,770
+unacetylimeramic acid بيطلع منهاأربع أمينو أسد
+
+25
+00:01:53,770 --> 00:01:57,470
+أربع أحماض أمينية زي ما أحنا شايفين بالصورة
+
+26
+00:01:57,470 --> 00:02:05,010
+مربوطين ب cross-link بـ peptide bond لو
+
+27
+00:02:05,010 --> 00:02:08,670
+بدي أعمل مقارنة بين الـ Gram positive bacteria و
+
+28
+00:02:08,670 --> 00:02:12,570
+Gram negative bacteria أول وجه مقارنة بـ Peptido
+
+29
+00:02:12,570 --> 00:02:15,630
+glycan layer اتفقنا لـ Gram positive bacteria
+
+30
+00:02:15,630 --> 00:02:19,600
+Multilayer of Peptido glycanلكن الـ Gram-negative
+
+31
+00:02:19,600 --> 00:02:23,380
+bacteria one layer of peptido-pig lichen تاني
+
+32
+00:02:23,380 --> 00:02:27,160
+الشيء الـ lipid and lipoprotein content الـ Gram
+
+33
+00:02:27,160 --> 00:02:30,980
+-positive bacteria low concentration of lipid لكن
+
+34
+00:02:30,980 --> 00:02:34,120
+الـ Gram-negative bacteria high concentration خاصة
+
+35
+00:02:34,120 --> 00:02:37,360
+في الـ outer membrane الـ outer membrane of the
+
+36
+00:02:37,360 --> 00:02:41,200
+cell wall فقط موجود في الـ Gram-negative bacteria
+
+37
+00:02:41,200 --> 00:02:44,600
+ومش موجود في الـ Gram-positive bacteria كذلك الـ
+
+38
+00:02:44,600 --> 00:02:47,540
+periplasmic membraneموجود في الـ Gram Negative
+
+39
+00:02:47,540 --> 00:02:50,560
+بكتيريا ومش موجود في الـ Gram Positive بكتيريا
+
+40
+00:02:50,560 --> 00:02:54,120
+البوليساكاريد السكريات في الـ Gram Positive
+
+41
+00:02:54,120 --> 00:02:58,180
+بكتيريا في عندي بوليساكاريد زي الـ Thycoic Acid
+
+42
+00:02:58,180 --> 00:03:01,500
+اللي بيطلع من الـ cell wall والـ Lipo Thycoic Acid
+
+43
+00:03:01,500 --> 00:03:04,760
+اللي بيطلع من ال cell membrane على عكس ال Gram
+
+44
+00:03:04,760 --> 00:03:08,180
+Negative بكتيريا ماعندي بوليساكاريد لكن في عندي
+
+45
+00:03:08,180 --> 00:03:11,940
+Lipopolysaccharide موجود في ال outer membrane
+
+46
+00:03:12,730 --> 00:03:16,610
+الليبو بوليساكريد من الليبو اتفقنا انه لجرام
+
+47
+00:03:16,610 --> 00:03:21,150
+negative بكتيريا high concentration of لبد خاصة في
+
+48
+00:03:21,150 --> 00:03:25,990
+ال outer membrane اخر وجه مقارنة التوكسين لجرام
+
+49
+00:03:25,990 --> 00:03:30,930
+positive بكتيريا بتفرز اكزو توكسين heat labile
+
+50
+00:03:30,930 --> 00:03:36,130
+toxin بيتأثر بدرجة الحرارة produced from some
+
+51
+00:03:36,130 --> 00:03:40,920
+species of living bacteriaLiving bacteria لأن
+
+52
+00:03:40,920 --> 00:03:46,200
+الاندوتوكسين اتفقنا بتفرز البكتيريا لما تموت from
+
+53
+00:03:46,200 --> 00:03:51,880
+dead bacteria بالنسبة لالإكزوتوكسين ممكن تفرزه
+
+54
+00:03:51,880 --> 00:03:55,420
+some species of gram negative bacteria لكن
+
+55
+00:03:55,420 --> 00:04:01,480
+الاندوتوكسين هو heat stable toxin produced only by
+
+56
+00:04:01,480 --> 00:04:05,000
+gram negative bacteria فقط بتفرزه لgram negative
+
+57
+00:04:05,000 --> 00:04:09,790
+bacteriaلأن الـ Endotoxin هو عبارة عن الـ Libid-A
+
+58
+00:04:09,790 --> 00:04:14,010
+اللي موجود في الـ Outer Membrane اللي موجود فقط في
+
+59
+00:04:14,010 --> 00:04:17,290
+الـ Gram Negative Bacteria الـ Outer Membrane
+
+60
+00:04:17,290 --> 00:04:23,270
+بيحتوي على Lipopolysaccharide اللي بتكون من Core
+
+61
+00:04:23,270 --> 00:04:27,030
+من Libid-A اللي هو عبارة عن الـ Endotoxin و بتكون
+
+62
+00:04:27,030 --> 00:04:31,370
+أيضا من الـ O Antigen الـ O Antigen هذا بيعمل
+
+63
+00:04:31,370 --> 00:04:37,410
+Antigenic Variation بيعمل تفراديعني لو دخل ميكروب
+
+64
+00:04:37,410 --> 00:04:41,990
+الجسم لو دخلت بكتيريا الجسم بيغير الانتجن بالتالي
+
+65
+00:04:41,990 --> 00:04:48,570
+ما بيتعرف على جهاز المنار بكتيريا
+
+66
+00:04:48,570 --> 00:04:52,310
+الcell wall بنلاحظ فيه الصورة في جرام بوزة
+
+67
+00:04:52,310 --> 00:04:56,610
+البكتيريا والجرام نكت البكتيريا بتحتوي على plasma
+
+68
+00:04:56,610 --> 00:05:01,650
+membrane لكن الفرق في ال cell wall في جرام بوزة
+
+69
+00:05:01,650 --> 00:05:05,330
+البكتيريا اللي بيبتيده أجلاي كان thickلكن في الـ
+
+70
+00:05:05,330 --> 00:05:08,750
+Gram-Negative اللي بيبتدوا as like and thin كذلك
+
+71
+00:05:08,750 --> 00:05:11,850
+أيضًا بنلاحظ أنه أنا في عندي في الـ Gram-Negative
+
+72
+00:05:11,850 --> 00:05:15,790
+بكتيريا outer membrane of cell wall مش موجود في
+
+73
+00:05:15,790 --> 00:05:21,170
+الـ Gram-Positive بكتيريا هذه
+
+74
+00:05:21,170 --> 00:05:25,130
+صورة توضحية للـ Gram-Positive بكتيريا في عندي cell
+
+75
+00:05:25,130 --> 00:05:29,270
+membrane بيبتدوا as like and layer، thick بالنسبة
+
+76
+00:05:29,270 --> 00:05:32,510
+للـ Polysaccharide في عندي Polysaccharide في ال
+
+77
+00:05:32,510 --> 00:05:36,180
+Gram-Positive بكتيرياالثيكوك أسد موجود في الـ cell
+
+78
+00:05:36,180 --> 00:05:39,940
+wall والليبوثيكوك أسد بيطلع من الـ cell membrane
+
+79
+00:05:39,940 --> 00:05:45,720
+بالأ لجرام نيجاتيف بكتيريا في cell membrane زي
+
+80
+00:05:45,720 --> 00:05:49,220
+لجرام positive بكتيريا ببتيدوز like هال layer of
+
+81
+00:05:49,220 --> 00:05:53,380
+cell wall 10 تحتوي على outer membrane of cell wall
+
+82
+00:05:53,380 --> 00:05:58,300
+اللي أحد مقويناته الليبو بولي ساكرايت اللي بيتكون
+
+83
+00:05:58,300 --> 00:06:03,300
+من ال lipid A اللي قولنا هو ال endotoxinأيضاً في
+
+84
+00:06:03,300 --> 00:06:06,960
+إن بيري إبلازمك اسباس في الـ Gram-negative بكتيريا
+
+85
+00:06:06,960 --> 00:06:13,200
+مش موجود في الـ Gram-positive بكتيريا كيف
+
+86
+00:06:13,200 --> 00:06:17,160
+تم اكتشاف الـ Gram-stain؟ في طبيب دنماركي اسمه
+
+87
+00:06:17,160 --> 00:06:21,240
+Christian Igram كان بيشتغل بالمختبر جاب بعض أنواع
+
+88
+00:06:21,240 --> 00:06:25,340
+البكتيريا جاب بكتيريا X وبكتيريا Y وصبغها
+
+89
+00:06:25,340 --> 00:06:29,000
+بـCrystal Violet الـCrystal Violet هي عبارة عن
+
+90
+00:06:29,000 --> 00:06:33,380
+بازكستان بتحمل شحنة موجبةهتصبغ البكتيريا سالبة
+
+91
+00:06:33,380 --> 00:06:38,140
+الشحنة انصبغت كل البكتيريا سواء كانت X أو Y بي الـ
+
+92
+00:06:38,140 --> 00:06:42,600
+Crystal Violet وأخدت اللون الـ Violet بعد كده أضاف
+
+93
+00:06:42,600 --> 00:06:47,220
+الـ Mordant المثبت الـ Iodine ثبت اللون داخل
+
+94
+00:06:47,220 --> 00:06:51,380
+البكتيريا تالت خطوة أضاف الـ Decolorizer مزيل
+
+95
+00:06:51,380 --> 00:06:56,940
+اللون مدة معينة عبارة عن كحول لاحظ أن اللون راح من
+
+96
+00:06:56,940 --> 00:07:02,000
+البكتيريا Yلكن بكتيريا X ضلت محتفظة باللون الفيولت
+
+97
+00:07:02,000 --> 00:07:06,880
+بعد كده أضاف صبغة تانية Basic Stain الصفرانين
+
+98
+00:07:06,880 --> 00:07:10,460
+البكتيريا اللي راح اللون منها أخدت الصبغة high
+
+99
+00:07:10,460 --> 00:07:13,580
+والبكتيريا اللي ما راح اللون منها ما أخدت الصبغة
+
+100
+00:07:13,580 --> 00:07:17,840
+ضلت محتفظة بالصبغة الأولى لcrystal violet ثم
+
+101
+00:07:17,840 --> 00:07:22,480
+البكتيريا اللي ضلت محتفظة باللون وما نزل وأخدت
+
+102
+00:07:22,480 --> 00:07:26,130
+اللون الفيولت بال gram positive bacteriaواللي أخدت
+
+103
+00:07:26,130 --> 00:07:29,670
+اللون الـ Pink وراح اللون منها بالـ Gram Negative
+
+104
+00:07:29,670 --> 00:07:34,510
+Bacteria السؤال، ليش البكتيريا الـ Gram Positive
+
+105
+00:07:34,510 --> 00:07:38,690
+Bacteria دلت محتفظة باللون الـ Violet؟ بناء على
+
+106
+00:07:38,690 --> 00:07:42,770
+المقارنة اللي عملناها ممكن نتفسر هذه النتيجة الـ
+
+107
+00:07:42,770 --> 00:07:46,230
+Gram Negative Bacteria بتتكون من طبقة واحدة من الـ
+
+108
+00:07:46,230 --> 00:07:50,150
+Peptidoglycan بالتالي سهل على الـ Decolorizer
+
+109
+00:07:50,150 --> 00:07:54,070
+الكحول يدخل ويزيل اللونلكن الـ Gram-positive
+
+110
+00:07:54,070 --> 00:07:57,330
+بكتيريا بتتكون من Multi-layer من الـ Betadoglycan
+
+111
+00:07:57,330 --> 00:08:01,990
+هتظل محتفظة باللون السبب التاني قلنا إنه أنا في
+
+112
+00:08:01,990 --> 00:08:05,030
+عندي في الـ Gram-negative بكتيريا كمية عالية من
+
+113
+00:08:05,030 --> 00:08:09,390
+الدهون High Rich of Lipid و الـ Decolorizer عبارة
+
+114
+00:08:09,390 --> 00:08:13,130
+عن كحول و الكحول بدوب في الدهون فرح يزيل اللون
+
+115
+00:08:13,130 --> 00:08:18,260
+منها و لما أصبغ بصبغة تانيةاللي هي الصفرانين إش
+
+116
+00:08:18,260 --> 00:08:21,360
+طبيعي إنها تنصبغي البكتيريا اللي مش مصبوغة لكن
+
+117
+00:08:21,360 --> 00:08:28,560
+البكتيريا المصبوغة هتظل لونها زي ما هو إجرام
+
+118
+00:08:28,560 --> 00:08:32,560
+أستان، differential أستان تفقنا إن الإجرام أستان
+
+119
+00:08:32,560 --> 00:08:36,380
+هي نوع من أنواع differential أستان اللي بتقسم
+
+120
+00:08:36,380 --> 00:08:41,240
+البكتيريا لمجموعات هانزي كريستيان إجرام أدنش دكتور
+
+121
+00:08:41,240 --> 00:08:44,580
+he developed a new method to stain bacteriaلذلك
+
+122
+00:08:44,580 --> 00:08:47,720
+يمكن أن يكونوا مشهورين في مجموعات مخصصة هذه
+
+123
+00:08:47,720 --> 00:08:52,160
+الكريستيان إيجرام طبيب دنماركي طور طريقة جديدة
+
+124
+00:08:52,160 --> 00:08:56,720
+لصباغة البكتيريا الـ Gram Stain اختلط البكتيريا
+
+125
+00:08:56,720 --> 00:09:01,460
+إلى عضو كبير قسمت البكتيريا لمجموع تاني ال Gram
+
+126
+00:09:01,460 --> 00:09:05,760
+Positive و Gram Negative هذا الهدف من ال Gram
+
+127
+00:09:05,760 --> 00:09:11,400
+Stainإجرام status is important in medicine the
+
+128
+00:09:11,400 --> 00:09:14,960
+presence or absence of a cell wall will change the
+
+129
+00:09:14,960 --> 00:09:19,200
+bacterium susceptibility to some antibiotic بقولنا
+
+130
+00:09:19,200 --> 00:09:23,540
+مهم جدا أعرف هل البكتيريا إجرام positive أو إجرام
+
+131
+00:09:23,540 --> 00:09:28,100
+negative why؟ لأن وجود أو غياب ال cell wall هيغير
+
+132
+00:09:28,100 --> 00:09:33,550
+استجابة البكتيريا للمضادات الحيوية كيف؟المضادات
+
+133
+00:09:33,550 --> 00:09:37,470
+الحيوية بتأثر على البكتيريا من خلال استهدافها
+
+134
+00:09:37,470 --> 00:09:42,670
+لتراكيب داخل الخلية ممكن تستهدف السلوال ممكن
+
+135
+00:09:42,670 --> 00:09:46,930
+البلوتين ممكن الريبوسان ممكن أي مكون داخل الخلية
+
+136
+00:09:46,930 --> 00:09:50,730
+بالنسبة للسلوال لأن الـ Gram Stain باعتمد عليه
+
+137
+00:09:50,730 --> 00:09:54,550
+قولنا إن السلوال بتكون من بيبتيدوجليكان
+
+138
+00:09:54,550 --> 00:09:57,650
+البيبتيدوجليكان بتكون من وحدات من
+
+139
+00:09:57,650 --> 00:10:01,850
+الـunacetylglucosamine و الـunacetylamuramic acid
+
+140
+00:10:02,200 --> 00:10:06,100
+الـ Unacetyl Muramic Acid بيطلع منها أحماض أمينية
+
+141
+00:10:06,100 --> 00:10:10,840
+هذه الأحماض مربوطة بالـ cross-link بـ peptide bond
+
+142
+00:10:10,840 --> 00:10:15,740
+في مضادات حيوية زي الـ penicillin بتستهدف هذه
+
+143
+00:10:15,740 --> 00:10:19,600
+الروابط و بتكسرها يعني بتستهدف فقط لـ gram
+
+144
+00:10:19,600 --> 00:10:23,040
+positive bacteria لأن اتفقنا لـ gram positive
+
+145
+00:10:23,040 --> 00:10:26,220
+bacteria multilayer of peptidoglycan هي الموجود
+
+146
+00:10:26,220 --> 00:10:30,000
+فيها الروابط لكن لـ gram negative طبقة واحدة
+
+147
+00:10:30,000 --> 00:10:35,610
+مافيها أي روابطيعني البنسلل لو أعطيته لمريض مصاب
+
+148
+00:10:35,610 --> 00:10:41,030
+بالتهاب بكتيريا موجبة إجرام هيستجيب لهذا المضاد
+
+149
+00:10:41,030 --> 00:10:45,090
+لكن لو أعطيته لمريض مصاب بالتهاب بكتيريا سالبة
+
+150
+00:10:45,090 --> 00:10:50,250
+إجرام ما رح يستجيب يبقى ضروري جدا أكون عارفة هل
+
+151
+00:10:50,250 --> 00:10:54,990
+البكتيريا موجبة إجرام أو سالبة إجرام لأن استجابتها
+
+152
+00:10:54,990 --> 00:10:56,530
+للمضادات هتختلف
+
+153
+00:11:01,040 --> 00:11:04,680
+almost all bacteria are described by their gram
+
+154
+00:11:04,680 --> 00:11:08,600
+stand characteristic تقريبا كل البكتيريا بيتم
+
+155
+00:11:08,600 --> 00:11:12,980
+ثباتتها بالgram stand إذا ملاحظين ما حكى all
+
+156
+00:11:12,980 --> 00:11:17,200
+bacteria قال almost all bacteria ليش لأن في عندي
+
+157
+00:11:17,200 --> 00:11:20,860
+بكتيريا اسمها ال micro bacterium هذه البكتيريا
+
+158
+00:11:20,860 --> 00:11:25,040
+موجودة على ال cell wall تبعها waxy lipid ال waxy
+
+159
+00:11:25,040 --> 00:11:29,780
+lipidبيعمل كحاجز لمانع دخول الصبغة فبالتالي ما
+
+160
+00:11:29,780 --> 00:11:33,400
+بقدر أصبغها بالـ Simple Stain ولا بالـ Gram Stain
+
+161
+00:11:33,400 --> 00:11:38,960
+هلجأ لطريقة أخرى لصبغتها بالـ Acid Fast Stain اللي
+
+162
+00:11:38,960 --> 00:11:43,600
+هنتعرف عليها في هذه المحاضرة Based on differences
+
+163
+00:11:43,600 --> 00:11:47,780
+of cell wall structure اتفقنا أن الصبغة بتكون حسب
+
+164
+00:11:47,780 --> 00:11:53,940
+الاختلاف في تركيب ال cell wall Reagent
+
+165
+00:11:53,940 --> 00:11:59,010
+for Gram StainCrystal Violet Primer Stain هي
+
+166
+00:11:59,010 --> 00:12:02,830
+الصبغة الأولية أول صبغة بستخدمها Positive Stain
+
+167
+00:12:02,830 --> 00:12:07,150
+يعني بتحمل شح نموجبة يعني Basic Stain عشان تصبغ
+
+168
+00:12:07,150 --> 00:12:12,170
+البكتيريا الصالبة Stain Cellule Parbol بتعطي اللون
+
+169
+00:12:12,170 --> 00:12:16,650
+البربل تاني مكوّن ال Iodine ال Iodine يعتبر
+
+170
+00:12:16,650 --> 00:12:21,290
+Mordant مثبتشو الـ principle تبع الـ Iodine بقول
+
+171
+00:12:21,290 --> 00:12:25,070
+ليه؟ combines with primary stain بيرتبط مع ال
+
+172
+00:12:25,070 --> 00:12:29,710
+primary stain اللي هي ال crystal violet to form an
+
+173
+00:12:29,710 --> 00:12:34,910
+insoluble complex عشان يكون insoluble complex ال
+
+174
+00:12:34,910 --> 00:12:38,790
+insoluble complex اسمه crystal violet iodine
+
+175
+00:12:38,790 --> 00:12:43,210
+complex هذا ال complex بينهجز داخل الcell wall
+
+176
+00:12:49,850 --> 00:12:53,850
+تالت مكون من المكونات لـ Gram Stain Kit الـ
+
+177
+00:12:53,850 --> 00:12:58,770
+Ethanol أو الـ Acetone يعتبر Decalarizer مزيل
+
+178
+00:12:58,770 --> 00:13:03,170
+لللون Crystal Violet Iodine Complex فاندي Complex
+
+179
+00:13:03,170 --> 00:13:06,950
+اسمه Crystal Violet Iodine Complex اللي هو الـ
+
+180
+00:13:06,950 --> 00:13:11,490
+Insoluble Complex washed out of a Gram Negative
+
+181
+00:13:11,490 --> 00:13:15,110
+Organism بقوللي هذا ال Complex هيروح من لجرام
+
+182
+00:13:15,110 --> 00:13:18,090
+Negative Bakteria ليش هيروح من لجرام Negative
+
+183
+00:13:18,090 --> 00:13:22,920
+Bakteriaبسبب أنه لا يمكن أن يكون مخطط من خلال
+
+184
+00:13:22,920 --> 00:13:26,200
+مجموعة ليبوغوليسا كارايد لايعني مش هيقدر ينهجز
+
+185
+00:13:26,200 --> 00:13:28,980
+لأنه أنا عندي في الإجرام نيجاتيف بكتيريا
+
+186
+00:13:28,980 --> 00:13:33,440
+ليبوغوليسا كارايد فيه عندي دهون والكحول هيتوب في
+
+187
+00:13:33,440 --> 00:13:39,680
+هذه الدهون ويزيل إلهم صفرانين الصفرانين بسميها ال
+
+188
+00:13:39,680 --> 00:13:43,380
+counter stand أو ال secondary stand simple
+
+189
+00:13:43,380 --> 00:13:48,100
+positive stand اتفقنا أنه هيSimple Stain لأنها
+
+190
+00:13:48,100 --> 00:13:52,080
+بازكستان بتحمل شحنة موجة بقى هتصبغ البكتيريا
+
+191
+00:13:52,080 --> 00:13:57,580
+السالبة ذات Provide Contrasting Dye قولي هي صبغة
+
+192
+00:13:57,580 --> 00:14:01,740
+مايوزية ليش سميتها هكذا لأن ال crystal violet
+
+193
+00:14:01,740 --> 00:14:05,500
+هتعطي اللون الviolet للكل سواء Gram Positive ولا
+
+194
+00:14:05,500 --> 00:14:10,720
+Gram Negative لكن الصفرانين هتميز بين ال Gram
+
+195
+00:14:10,720 --> 00:14:14,940
+Positive و Gram Negativeالـ Gram Positive هتضلها
+
+196
+00:14:14,940 --> 00:14:17,980
+محتفظة باللون الـ Violet لكن الـ Gram Negative
+
+197
+00:14:17,980 --> 00:14:23,180
+هيروح منها اللون هترجح شفافة ففضلي أحط لون يميزها
+
+198
+00:14:23,180 --> 00:14:28,440
+الصفرانين ميزها وأعطاها هذا اللون for decolorized
+
+199
+00:14:28,440 --> 00:14:31,700
+cell هتعطي اللون للبكتيريا اللي راح اللون منها
+
+200
+00:14:31,700 --> 00:14:37,220
+يعني للـ Gram Negative بكتيريا Stains or سلبه اللي
+
+201
+00:14:37,220 --> 00:14:41,620
+بتصبح كل الخلايا but only the negative ones
+
+202
+00:14:41,840 --> 00:14:46,360
+actually a beer pink لكن فقط الـ gram negative
+
+203
+00:14:46,360 --> 00:14:52,580
+بكتيريا هي اللي هتاخد هاي الصبغة و تنسبغ باللون ال
+
+204
+00:14:52,580 --> 00:14:56,320
+pink ليش هتسبغ كل الخلايا لأن بالاصل هي basic
+
+205
+00:14:56,320 --> 00:15:00,280
+stain لكن ال gram positive بكتيريا ما أخدت اللون
+
+206
+00:15:00,280 --> 00:15:06,030
+لأن هي بالاصل ملونة بالنسبة للجدوللو انا صبغت الـ
+
+207
+00:15:06,030 --> 00:15:08,570
+Gram Positive و Gram Negative بيه الـ Primary
+
+208
+00:15:08,570 --> 00:15:11,950
+Stain لـ Crystal Violet سواء كانت Positive او
+
+209
+00:15:11,950 --> 00:15:15,150
+Negative هتاخد اللون الـ purple الـ Mordant
+
+210
+00:15:15,150 --> 00:15:19,490
+الأيودين هيثبت هذا اللون لما اضيف الـ Decolorizing
+
+211
+00:15:19,490 --> 00:15:23,510
+Agent الكحول او الاسيتون مزيل اللون ال Gram
+
+212
+00:15:23,510 --> 00:15:27,450
+Positive بكتيريا هتظل محتفظة باللون لكن ال Gram
+
+213
+00:15:27,450 --> 00:15:31,690
+Negative بكتيريا هيروح اللون منها ويعرفنا ليش
+
+214
+00:15:31,690 --> 00:15:36,140
+السببضفت الـ counter stain الصفرانين لجرام
+
+215
+00:15:36,140 --> 00:15:39,680
+positive بكتيريا هتظل محتفظة باللون لكن لجرام
+
+216
+00:15:39,680 --> 00:15:45,680
+negative هتاخد هذه الصبغة لأنه صارت شفافة فهتاخد
+
+217
+00:15:45,680 --> 00:15:53,560
+هذا اللون تعرفنا
+
+218
+00:15:53,560 --> 00:15:58,000
+على الهدف من لجرام stain و ال principle لجرام
+
+219
+00:15:58,000 --> 00:16:03,100
+stain هلأ بنتعرف على خطوات عمل لجرام stain قلناإنه
+
+220
+00:16:03,100 --> 00:16:06,560
+أي استخدام في الـ Microbiology Lab أول تلات خطوات
+
+221
+00:16:06,560 --> 00:16:10,760
+تابتة أول خطوة الـ Smear preparation وهي تختلف على
+
+222
+00:16:10,760 --> 00:16:14,960
+حسب إذا كان من مجموعة أو من البلاد تاني خطوة الـ
+
+223
+00:16:14,960 --> 00:16:19,060
+Air-drying to prevent lysis تالت خطوة الـ Heat
+
+224
+00:16:19,060 --> 00:16:23,280
+-fixation to let bacteria stick into slide and to
+
+225
+00:16:23,280 --> 00:16:27,800
+kill bacteria الرسمة الموجودة في هذه الصفحة
+
+226
+00:16:27,800 --> 00:16:32,610
+شرحناها نفس ما شرحناها في الصفحة السابقةأول أشياء
+
+227
+00:16:32,610 --> 00:16:36,710
+أن الخلايا كانت transparent هي البكتيريا قلنا أنه
+
+228
+00:16:36,710 --> 00:16:40,810
+من خصائصها transparent شفافة صبغتها بـ crystal
+
+229
+00:16:40,810 --> 00:16:43,830
+violet أخدت اللون سواء إجرام positive أو إجرام
+
+230
+00:16:43,830 --> 00:16:47,650
+negative ضفت الـ decolorizer اللي هو الكفول أو
+
+231
+00:16:47,650 --> 00:16:51,010
+الاسيتون اللون راح من الإجرام negative لكن الإجرام
+
+232
+00:16:51,010 --> 00:16:55,690
+positive دلت محتفظة باللون الviolet ضفت الصفرانين
+
+233
+00:16:55,690 --> 00:16:59,810
+أخدت اللون البكتيريا اللي راح اللون منها اللي هي
+
+234
+00:16:59,810 --> 00:17:01,530
+الإجرام negative بكتيريا
+
+235
+00:17:07,060 --> 00:17:10,680
+الخطوة الرابعه هي الاستان وقلنا انها تختلف حسب نوع
+
+236
+00:17:10,680 --> 00:17:14,100
+الاستان سيمبل استان اجرام استان اصد فاست استان
+
+237
+00:17:14,100 --> 00:17:18,300
+بالنسبالى لجرام استان اول اشيه application of a
+
+238
+00:17:18,300 --> 00:17:21,700
+crystal violet بدنا نضيف ال crystal violet ل
+
+239
+00:17:21,700 --> 00:17:27,460
+primary استان لمدة one minute بعد ما تخلص الدقيقة
+
+240
+00:17:27,460 --> 00:17:32,260
+هغسل بالتعب water تاني اشيه هضيف ال mordant المثبت
+
+241
+00:17:32,260 --> 00:17:36,900
+الايودين برضه لمدة دقيقةبعد ما اتخلص اللي دي هغسل
+
+242
+00:17:36,900 --> 00:17:41,820
+بال water بعدها هضيف الكحول او ال acetone اللي هو
+
+243
+00:17:41,820 --> 00:17:46,360
+ال decolorizer مزيل اللون لكن هانقل وقت 15 second
+
+244
+00:17:46,360 --> 00:17:50,880
+بعد ما اتخلص ال 15 second هغسل بال water اخر خطوة
+
+245
+00:17:50,880 --> 00:17:54,340
+هضيف ال counterstain او ال secondary stain اللي هي
+
+246
+00:17:54,340 --> 00:17:59,100
+السفرانية لمدة one minute بعد ما اخلص هغسل بال
+
+247
+00:17:59,100 --> 00:18:03,520
+water هتكها Jeff Air Drying بعدين اشوفها تحت ال
+
+248
+00:18:03,520 --> 00:18:13,310
+microscope على عدسة40X و100XI بالنسبة
+
+249
+00:18:13,310 --> 00:18:17,750
+للنتائج كيف بدي أكتب نتيجة لـ Gram Stain نتيجة لـ
+
+250
+00:18:17,750 --> 00:18:21,670
+Gram Stain بتتكون من شقين الشق الأول بتعلق هل هى
+
+251
+00:18:21,670 --> 00:18:25,950
+Gram Positive أو Gram Negative لو كان اللون Violet
+
+252
+00:18:25,950 --> 00:18:29,550
+بقى هى Gram Positive لكن لو كان اللون Pink أو Red
+
+253
+00:18:29,550 --> 00:18:33,550
+بقى هى Gram Negativeالشق التاني بيتعلق بال shape
+
+254
+00:18:33,550 --> 00:18:39,070
+هل هي بصلاي عصاوية أو كوكاي كراوية يبقى لو أجيناها
+
+255
+00:18:39,070 --> 00:18:42,230
+في هذه الصورة بدي أكتب نتيجة لـ Gram Stain هشوف
+
+256
+00:18:42,230 --> 00:18:45,910
+اللون اللون Red يبقى هي Gram Negative Bacteria
+
+257
+00:18:45,910 --> 00:18:50,210
+هطلع على شكل البكتيريا هي عصاوية يبقى بصلاي يبقى
+
+258
+00:18:50,210 --> 00:18:54,290
+النتيجة Gram Negative Bacilli بدون ما أكتب ال
+
+259
+00:18:54,290 --> 00:18:58,540
+arrangementالصورة التانية اللون violet يبقى هي
+
+260
+00:18:58,540 --> 00:19:02,480
+إجرام positive بكتيريا الشكل كروي يبقى كوكالي
+
+261
+00:19:02,480 --> 00:19:11,260
+النتيجة كاملة إجرام positive كوكالي error
+
+262
+00:19:11,260 --> 00:19:15,720
+during staining never ever used oil culture بقول
+
+263
+00:19:15,720 --> 00:19:20,280
+ممنوع أستخدم مستعمرات من مزارة قديمةلأس لأن
+
+264
+00:19:20,280 --> 00:19:24,080
+البكتيريا مع طول المدة الجدار الخلوي تبعها بيبدأ
+
+265
+00:19:24,080 --> 00:19:28,560
+يتحلل بالتالي هي بتكون Multilayer اتحلل الجزء منها
+
+266
+00:19:28,560 --> 00:19:32,340
+صارت مبينة كإنها One Layer يعني بيعطيني False
+
+267
+00:19:32,340 --> 00:19:35,380
+Result يعني ممكن البكتيريا تكون بالاصل Gram
+
+268
+00:19:35,380 --> 00:19:38,900
+Positive بكتيريا روحت عملتي Gram Stain أخدت
+
+269
+00:19:38,900 --> 00:19:44,660
+البكتيريا من Old Culture هتبين في نتيجة Gram Stain
+
+270
+00:19:44,660 --> 00:19:49,290
+كإنها Gram Negative هتبين لونها لون Pinkفبالتالي
+
+271
+00:19:49,290 --> 00:19:55,550
+ممنوع أنا أستخدم بكتيريا فيه لـ Gram-S تكون من All
+
+272
+00:19:55,550 --> 00:19:59,750
+culture never ever used sample for patient take
+
+273
+00:19:59,750 --> 00:20:03,130
+antibiotic اللي ما تاخدي بكتيريا معزولة من مريض
+
+274
+00:20:03,130 --> 00:20:06,590
+بياخد antibiotic أحنا قلنا في أول المحاضرة أن
+
+275
+00:20:06,590 --> 00:20:10,390
+المضادات الحيوية ممكن تستهدف ال cell wall بالتحديد
+
+276
+00:20:10,390 --> 00:20:13,830
+بتستهدف ال cross link زي ال penicillin اللي
+
+277
+00:20:13,830 --> 00:20:18,590
+بيستهدف ل gram positive بكتيريا يعنيلو مريض بياخد
+
+278
+00:20:18,590 --> 00:20:23,750
+penicillin عشان يعالج التهاب بكتيري من البكتيريا
+
+279
+00:20:23,750 --> 00:20:29,790
+لجرام positive bacteria هتبين كإنها في جرام
+
+280
+00:20:29,790 --> 00:20:34,010
+negative bacteria في الجرام استخدامي، ليش؟ لأن الـ
+
+281
+00:20:34,010 --> 00:20:36,810
+penicillin بيستهدف الـ cross-link هي كانت
+
+282
+00:20:36,810 --> 00:20:40,830
+multilayer of peptidoglycan هتبين كإنها one layer
+
+283
+00:20:40,830 --> 00:20:45,790
+هيفكك الروابط، هيعمل collapse لـ cell wallTime of
+
+284
+00:20:45,790 --> 00:20:50,510
+Decalarizer بقولنا وقت الـ Decalarizer مهم جدا
+
+285
+00:20:50,510 --> 00:20:55,890
+يعتبر critical لو زودت انا over ال gram positive
+
+286
+00:20:55,890 --> 00:21:00,330
+بكتيريا هتبين كأنها gram negative بكتيريا ليش؟
+
+287
+00:21:00,330 --> 00:21:03,790
+لأنه هيكون في عندي وقت كافي لدخول ال Decalarizer
+
+288
+00:21:03,790 --> 00:21:07,910
+اللي هو الكحول لأ ال multilayer فبالتالي اللون
+
+289
+00:21:07,910 --> 00:21:12,390
+هيروح من ال gram positive بكتيرياهتصبح البكتيريا
+
+290
+00:21:12,390 --> 00:21:16,330
+شفّا فهسبغ بالصفرانين هتاخد اللون ال pink وهتبين
+
+291
+00:21:16,330 --> 00:21:21,030
+كإنها gram negative بكتيريا لو أنا قللت وقت ال
+
+292
+00:21:21,030 --> 00:21:25,350
+decolorizer أقل من 15 second لgram negative
+
+293
+00:21:25,350 --> 00:21:29,550
+بكتيريا هتبين كإنها gram positive بكتيريا ليش؟
+
+294
+00:21:29,550 --> 00:21:33,690
+لأنه مش هيكون في وقت كافي عشان ينزل ال crystal
+
+295
+00:21:33,690 --> 00:21:37,470
+violet من لgram negative بكتيريا من الpeptido
+
+296
+00:21:37,470 --> 00:21:40,930
+glycanهتبقى الـ Gram Negative محتفظة باللون
+
+297
+00:21:40,930 --> 00:21:44,990
+الفيوليت روحت أنا حطيت الصفرانين مش هتاخد الصبغة
+
+298
+00:21:44,990 --> 00:21:49,630
+لأنها ملونة باللون الفيوليت فبالتالي الـ Gram
+
+299
+00:21:49,630 --> 00:21:53,690
+Negative هتبين كإنها Gram Positive time of
+
+300
+00:21:53,690 --> 00:21:57,850
+fixation لو أنا زودت وقت ال fixation أو ضلت حتى ال
+
+301
+00:21:57,850 --> 00:22:01,190
+slide على اللهب ال Gram Positive بكتيريا هتبين
+
+302
+00:22:01,190 --> 00:22:05,350
+كإنها Gram Negative بكتيرياأحنا قلنا الفكساشن
+
+303
+00:22:05,350 --> 00:22:09,930
+بيعمل partial melting of cell wall لو أنا زودت وقت
+
+304
+00:22:09,930 --> 00:22:13,430
+الفكساشن هيصير في عندي complete melting للcell
+
+305
+00:22:13,430 --> 00:22:19,270
+wall يبقى هتبين ال multilayer كأنها one layer
+
+306
+00:22:19,270 --> 00:22:24,150
+هيصير collapse لل cell wall law no sample on slide
+
+307
+00:22:24,150 --> 00:22:28,030
+أحنا قلنا وظيفة الفكساشن عشان تنتصق البكتيريا على
+
+308
+00:22:28,030 --> 00:22:32,110
+ال slide فبالتالي لو كان قليل مش هتنتصق علىالـ
+
+309
+00:22:32,110 --> 00:22:36,310
+slide يبقى مش هيكون فيه عندي عينة هذه أهم الأخطاء
+
+310
+00:22:36,310 --> 00:22:40,750
+اللي ممكن تحصل خلال عملية Gram Staining عشان هيك
+
+311
+00:22:40,750 --> 00:22:45,330
+لازم نلتزم بالوقت في كل خطوة هيك بنكون خلصنا أول
+
+312
+00:22:45,330 --> 00:22:49,210
+نوع من أنواع الـDifferential Stain اللي هو Gram
+
+313
+00:22:49,210 --> 00:22:49,810
+Stain
+
diff --git a/PL9fwy3NUQKwZWgAb_V1ATlRRsH-6i3cPC/PYxwZLDpRfI_raw.srt b/PL9fwy3NUQKwZWgAb_V1ATlRRsH-6i3cPC/PYxwZLDpRfI_raw.srt
new file mode 100644
index 0000000000000000000000000000000000000000..c8ce12abdccdcc120c50534e0ef39db6ce23661f
--- /dev/null
+++ b/PL9fwy3NUQKwZWgAb_V1ATlRRsH-6i3cPC/PYxwZLDpRfI_raw.srt
@@ -0,0 +1,1900 @@
+1
+00:00:03,040 --> 00:00:06,920
+السلام عليكم ورحمة الله وبركاته يعطيكم العافية
+
+2
+00:00:06,920 --> 00:00:11,440
+جميعا نستكمل معاكم محاضرات مساق الـ Microbiology
+
+3
+00:00:11,440 --> 00:00:16,620
+العملي وفي آخر محاضرة في هذا المساق هنتكلم في هذه
+
+4
+00:00:16,620 --> 00:00:20,240
+المحاضرة عن موضوعين الموضوع الأول بكتيريا
+
+5
+00:00:20,240 --> 00:00:24,540
+generation time generation time هو الوقت اللازم
+
+6
+00:00:24,540 --> 00:00:29,410
+البكتيريا حتى تنقسم وتضاعفبالنسبة للموضوع التاني
+
+7
+00:00:29,410 --> 00:00:34,750
+Microbial Control Agent العوامل اللي بتتحكم في نمو
+
+8
+00:00:34,750 --> 00:00:48,110
+الميكروبات إما بالتثبيت أو بالقتل Growth
+
+9
+00:00:48,110 --> 00:00:53,430
+النمو، إيش هو النمو؟ is an orderly increase in the
+
+10
+00:00:53,430 --> 00:00:58,610
+quantity of cellular constituentsالنمو هو زيادة في
+
+11
+00:00:58,610 --> 00:01:04,550
+محتويات الخلية البكتيرية زيادة في الـ Biomass في
+
+12
+00:01:04,550 --> 00:01:09,350
+الكتلة الحيوية للخلية النمو على إيش بيعتمد It
+
+13
+00:01:09,350 --> 00:01:12,890
+depends upon the ability of the cell to form new
+
+14
+00:01:12,890 --> 00:01:16,330
+protoplasm from nutrients available in the
+
+15
+00:01:16,330 --> 00:01:21,050
+environment بيقول بيعتمد على قدرة الخلية إنها
+
+16
+00:01:21,050 --> 00:01:26,850
+اتكون protoplasm جديد protoplasm يعني cytoplasmمن
+
+17
+00:01:26,850 --> 00:01:31,010
+المواد الغذائية الموجودة في البيئة المحيطة بالخلية
+
+18
+00:01:31,010 --> 00:01:36,010
+البكتيريا بقولي المواد البكتيريا بعض البكتيريا الـ
+
+19
+00:01:36,010 --> 00:01:40,690
+growth إنه هو بيعتمد على increase أو بيضم increase
+
+20
+00:01:40,690 --> 00:01:44,830
+in cell mass قولنا ال growth هو زيادة في الكتلة
+
+21
+00:01:44,830 --> 00:01:49,030
+الحيوية مش بس زيادة في الكتلة الحيوية بقولي كمان
+
+22
+00:01:49,030 --> 00:01:54,590
+and number of ribosomes زيادة اللي هو ال ribosomes
+
+23
+00:01:54,590 --> 00:01:58,130
+عدد ال ribosomesduplication of the bacterial
+
+24
+00:01:58,130 --> 00:02:02,110
+chromosome أكيد هيصير في عندي تضاعف للمادة
+
+25
+00:02:02,110 --> 00:02:07,490
+الوراثية للبكتيريا لأنه بنهاية هيتكون عندي خليتين
+
+26
+00:02:07,490 --> 00:02:11,610
+بكتيريتين كل بكتيرية هتاخد اي chromosome synthesis
+
+27
+00:02:11,610 --> 00:02:15,770
+of a new cell wall and plasma membrane برضه هيتكون
+
+28
+00:02:15,770 --> 00:02:19,150
+cell wall جديد و plasma membrane جديد لأنه احنا
+
+29
+00:02:19,150 --> 00:02:25,600
+قلنا هيتكون عنديto cell فبالتالي كل خلية بكتيرية
+
+30
+00:02:25,600 --> 00:02:31,100
+هيكون لها cell wall منفصل plasma membrane منفصل
+
+31
+00:02:31,100 --> 00:02:37,320
+مكونات chromosome منفصل كمان بقولي partitioning of
+
+32
+00:02:37,320 --> 00:02:43,100
+the two chromosomes هيصير في عندي انه انقسام لـ
+
+33
+00:02:43,100 --> 00:02:46,760
+chromosomes ل two chromosomes لأن كل خلية بكتيرية
+
+34
+00:02:46,760 --> 00:02:51,290
+هتاخد chromosomeSeptum Formation مايعني سبتم
+
+35
+00:02:51,290 --> 00:02:55,950
+فورماشن يعني اللي هو هيتكون عندي حاجز لما يصير قبل
+
+36
+00:02:55,950 --> 00:02:59,110
+ما يصير في عندي cell division هيصير في عندي تكوين
+
+37
+00:02:59,110 --> 00:03:04,270
+حاجز تمام بعدها هيصير عندي الانقسام هنشوف في ال
+
+38
+00:03:04,270 --> 00:03:08,890
+slide اللي بعيدها شو هو هذا الحاجز بالنهاية هتنقسم
+
+39
+00:03:08,890 --> 00:03:12,530
+الخلية البكتيرية لخليتين بكتيريتين هيصير في عندي
+
+40
+00:03:12,530 --> 00:03:17,040
+cell divisionاللي هو الانقسام بواسطة الـ Binary
+
+41
+00:03:17,040 --> 00:03:21,360
+Evasion اللي هو الانشطار الثنائي شو هو الانشطار
+
+42
+00:03:21,360 --> 00:03:24,940
+الثنائي؟ بقول لي this a sexual process of
+
+43
+00:03:24,940 --> 00:03:28,360
+reproduction is called binary evasion اللي هي
+
+44
+00:03:28,360 --> 00:03:34,480
+بتكون عملية لا جنسية مش sexual، a sexual process
+
+45
+00:03:34,480 --> 00:03:39,400
+لأنه بالنهاية أنا بتكون أو الانقسام بيصير من خلية
+
+46
+00:03:39,400 --> 00:03:44,890
+واحدة من mother cells مش من خليتينمش من أب و أم
+
+47
+00:03:44,890 --> 00:03:49,950
+فبالتالي هي لاجنسية عملية لاجنسية asexual process
+
+48
+00:03:49,950 --> 00:03:53,470
+اللي هي ال binary effusion اللي هي الانشطار
+
+49
+00:03:53,470 --> 00:03:58,230
+الثنائي كيف
+
+50
+00:03:58,230 --> 00:04:02,790
+بيصير ال binary division أو ال cell division لخلية
+
+51
+00:04:02,790 --> 00:04:06,250
+البكتيريا نلاحظ في الصورة أن أنا في عندي خلية
+
+52
+00:04:06,250 --> 00:04:12,410
+بكتيرية فيها ال DNA المادة الوراثيةالـ DNA هيصير
+
+53
+00:04:12,410 --> 00:04:16,310
+له اللي هو تضاعف عندي هيصير DNA replication نلاحظ
+
+54
+00:04:16,310 --> 00:04:20,490
+في الصورة إن والله اتضاعف اللي هو الـ Circular DNA
+
+55
+00:04:20,490 --> 00:04:25,550
+طبعا خلية البكتيريا قلنا في أول معمل هو عبارة عن
+
+56
+00:04:25,550 --> 00:04:30,670
+Circular دائري مش linear زي الإنسان هيصير في عندي
+
+57
+00:04:30,670 --> 00:04:35,220
+DNA replication تضاعف للـ DNAهيصير فيه cell
+
+58
+00:04:35,220 --> 00:04:39,840
+elongation هيصير استطالة لخلية البكتيريا بعد كده
+
+59
+00:04:39,840 --> 00:04:44,300
+البكتيريا هتحضر حالها للانقسام هيصير في عينيه
+
+60
+00:04:44,300 --> 00:04:48,860
+يقولنا تكون حاجز symptom formation اللي هو يتكون
+
+61
+00:04:48,860 --> 00:04:54,780
+في عينيه حاجز حتى إن الخلية البكتيرية تحضر حالها
+
+62
+00:04:54,780 --> 00:05:00,580
+للانقسام لخليتين بشكل كامل وتبتعد عن بعض بنلاحظ في
+
+63
+00:05:00,580 --> 00:05:06,030
+الصورة هنا إنهصار في عندي اللي هو انفصال لخليتين
+
+64
+00:05:06,030 --> 00:05:09,890
+البكتيريتين عن بعض اللي هو الـ two daughter cell
+
+65
+00:05:09,890 --> 00:05:14,170
+separated بعدها صار في عندي اللي هو كل خلية
+
+66
+00:05:14,170 --> 00:05:19,130
+بكتيرية انفصلت صار لها DNA خاص فيها وزي ما ملاحظ
+
+67
+00:05:19,130 --> 00:05:24,150
+عبارة عن اللي هو Circular دائري طبعاً لها cell
+
+68
+00:05:24,150 --> 00:05:27,930
+wall خاص فيها cytoplasm خاص فيها cell membrane خاص
+
+69
+00:05:27,930 --> 00:05:31,070
+فيها chromosome خاص فيها زي ما اتكلمنا في ال slide
+
+70
+00:05:31,070 --> 00:05:39,940
+اللي قبلهذا بالنسبة لـ Cell Division في
+
+71
+00:05:39,940 --> 00:05:44,340
+هاي الـ slide هنتعرف على بعض المصطلحات الهمة أول
+
+72
+00:05:44,340 --> 00:05:47,820
+مصطلح اللي هو الـ Growth بالنسبة لـ Growth عرفناه
+
+73
+00:05:47,820 --> 00:05:51,940
+في الـ slide الأبل وقلنا اللي هو زيادة في الكتلة
+
+74
+00:05:51,940 --> 00:05:56,460
+الحيويةThe Equation of Biomass اللي هو زيادة في
+
+75
+00:05:56,460 --> 00:06:00,240
+الكتلة الحيويةleading to cell revision بالنهاية
+
+76
+00:06:00,240 --> 00:06:04,920
+هيصير عندي انقسام لالخلية لخليتين بكتيريتين or
+
+77
+00:06:04,920 --> 00:06:09,920
+reproduction تاني مصطلح a batch culture ال batch
+
+78
+00:06:09,920 --> 00:06:14,200
+culture is a closed system نظام مغلق in a broth
+
+79
+00:06:14,200 --> 00:06:18,020
+medium in which no additional nutrient واللي هو
+
+80
+00:06:18,020 --> 00:06:22,040
+نظام مغلق في ال broth media مايصير في عندي أي
+
+81
+00:06:22,040 --> 00:06:26,550
+إضافات للمواد الغذائيةBad is added after
+
+82
+00:06:26,550 --> 00:06:30,230
+incubation of the brood بعد ما أنا أزرع هذه
+
+83
+00:06:30,230 --> 00:06:34,610
+البكتيريا يعني بكون عندي ميديا وبزرع فيها
+
+84
+00:06:34,610 --> 00:06:40,610
+البكتيريا وبحطها في ال incubator لو خلصت المغذيات
+
+85
+00:06:40,610 --> 00:06:44,050
+و البكتيريا في ال incubator أو بعد ما أنا طلعت هذا
+
+86
+00:06:44,050 --> 00:06:48,630
+الطبق من ال incubator بتموت البكتيريا لكن هذا
+
+87
+00:06:48,630 --> 00:06:52,800
+بالنسبة لبatch culture في النظام المغلقفي النظام
+
+88
+00:06:52,800 --> 00:06:57,320
+المغلق أنا ما بضيف أي مغذيات بعد اللي هو الزراعة
+
+89
+00:06:57,320 --> 00:07:02,460
+خلاص إذا خلصت المغذيات البكتيريا بتموت لكن بالنسبة
+
+90
+00:07:02,460 --> 00:07:06,340
+لـ continuous culture في عندي أنا نظام مفتوح حد
+
+91
+00:07:06,340 --> 00:07:10,580
+نظام كل ما اتخلص المواد الغذائية أنا بضيف غيرها
+
+92
+00:07:10,580 --> 00:07:15,960
+فبالتالي في نظام ال continuous culture ما بتمر
+
+93
+00:07:15,960 --> 00:07:20,510
+البكتيريا بمرحلة اللي هو ال death phaseفي مرحلة
+
+94
+00:07:20,510 --> 00:07:24,150
+الموت لأني أنا بصير أضيف مواد غذائية لكن في الـ
+
+95
+00:07:24,150 --> 00:07:27,830
+Closed System اللي هو الـ Batch Culture ما بضيف أي
+
+96
+00:07:27,830 --> 00:07:32,350
+مغذية No additional nutrient بعد الزراعة فبالتالي
+
+97
+00:07:32,350 --> 00:07:36,290
+البكتيريا بتموت و بتمر بمرحلات اللي هو الـ Death
+
+98
+00:07:36,290 --> 00:07:41,250
+phase بالنسبة للـ Generation Time اتكلمنا عنه Time
+
+99
+00:07:41,250 --> 00:07:44,510
+taken for a cell population to double in number هو
+
+100
+00:07:44,510 --> 00:07:49,200
+الوقت اللي لازم لاقى الخلية انها تتضاعف في العددهو
+
+101
+00:07:49,200 --> 00:07:52,600
+بقول اللي بيساوي the average length of the cell
+
+102
+00:07:52,600 --> 00:07:59,120
+cycle هذا بالنسبة لهذه المصطلحات اللي هتمر معناها
+
+103
+00:07:59,120 --> 00:08:05,340
+خلال الـ chapter الـ
+
+104
+00:08:05,340 --> 00:08:09,680
+bacterial growth curve اللي هو منحنة نمو البكتيريا
+
+105
+00:08:09,680 --> 00:08:14,700
+في عندي أربع أطوار أو أربع stage بتمر فيهم الخلية
+
+106
+00:08:14,700 --> 00:08:18,910
+البكتيرية في النموfour characteristic phase of the
+
+107
+00:08:18,910 --> 00:08:22,910
+growth cycle اللي هم ال lag phase ال log phase ال
+
+108
+00:08:22,910 --> 00:08:27,050
+stationary phase ال death phase هنتكلم عن كل phase
+
+109
+00:08:27,050 --> 00:08:31,070
+بالتفصيل أول phase اللي هو ال lag phase اللي هو
+
+110
+00:08:31,070 --> 00:08:35,650
+التطور التحضيري للانقسام هذا التطور بنعطي فرصة
+
+111
+00:08:35,650 --> 00:08:38,970
+للبكتيريا إنها تتأقلم إنه يصير في عندي adaptation
+
+112
+00:08:38,970 --> 00:08:44,130
+تحضر حالها للانقسام تحضر الانزيمات للانقساملكن
+
+113
+00:08:44,130 --> 00:08:47,370
+فعليًا ما بيصير في عندي أي نمو ما بيصير عندي أي
+
+114
+00:08:47,370 --> 00:08:52,630
+انقسام ما بيصير عندي زيادة في العدد متى هذا ال
+
+115
+00:08:52,630 --> 00:08:56,350
+fest بيصير immediately after the incubation of the
+
+116
+00:08:56,350 --> 00:09:00,190
+cell into fresh media بقوللي وقت ما أنتي تزرعي
+
+117
+00:09:00,190 --> 00:09:04,390
+الخلايا في ال fresh media هانبتبدأ مرحلة ال lag
+
+118
+00:09:04,390 --> 00:09:08,350
+fest ال population بقوللي ال population remained
+
+119
+00:09:08,350 --> 00:09:13,670
+temporarily unchanged بقوللي ما بيصير في عنديأي
+
+120
+00:09:13,670 --> 00:09:17,790
+تغيير في العدد تمام ما بيصير أي تغيير في العدد
+
+121
+00:09:17,790 --> 00:09:22,030
+بشكل مؤقت although there is no apparent cell
+
+122
+00:09:22,030 --> 00:09:25,050
+division بقولي ما بيصير في عندي أي انقسام اتفاقنة
+
+123
+00:09:25,050 --> 00:09:28,570
+ما بيصير في عندي أي نمو the cell may be growing in
+
+124
+00:09:28,570 --> 00:09:33,230
+volume لكن ممكن الخلايا يصير في عندي زيادة في
+
+125
+00:09:33,230 --> 00:09:37,430
+الحجم ال volume وليس العدد ما بيصير في عندي زيادة
+
+126
+00:09:37,430 --> 00:09:42,000
+في العدد قولنا ال population بيظلواطبعاً بشكل مؤقت
+
+127
+00:09:42,000 --> 00:09:46,040
+ثابت ما بتغير لكن بقولي ممكن يصير في عندي زيادة في
+
+128
+00:09:46,040 --> 00:09:50,280
+الحجم في ال biomass لكن مش في العدد بيصير في عندي
+
+129
+00:09:50,280 --> 00:09:54,240
+synthesizing لإنزايم أكيد بتحضر حالها لإنقسام
+
+130
+00:09:54,240 --> 00:10:00,300
+فبالتالي بيتم إنتاج إنزيمات بروتين RNA increasing
+
+131
+00:10:00,300 --> 00:10:04,700
+in metabolic activity كل هذه الأمور بتصير عشان
+
+132
+00:10:04,700 --> 00:10:09,180
+تحضر حالها لإنقسام لكن ما بيصير في عندي أي نمو
+
+133
+00:10:17,300 --> 00:10:21,120
+هذه الرسمة بتعبر عن مراحل نمو البكتيريا قلنا فيه
+
+134
+00:10:21,120 --> 00:10:26,000
+عندي four stages lag-log stationary death phase
+
+135
+00:10:26,000 --> 00:10:31,000
+أول مرحلة أو أول stage اللي هو الـ lag phase قلنا
+
+136
+00:10:31,000 --> 00:10:36,380
+إنه عدد البكتيريا في هذا الطور بيظل ثابت un
+
+137
+00:10:36,380 --> 00:10:41,740
+-exchanged طبعا أنا عندي في هذا المحور اللي هو
+
+138
+00:10:41,740 --> 00:10:45,640
+الـtime وهذا المحور عدد البكتيريا عدد البكتيريا في
+
+139
+00:10:45,640 --> 00:10:50,470
+الـ lag phaseبيظلوا ما بيتغير بيظلوا ثابت زي ما
+
+140
+00:10:50,470 --> 00:10:54,150
+بنلاحظ في الصورة انه العدد ثابت في مرحلة ال lag
+
+141
+00:10:54,150 --> 00:10:58,550
+phase بقوللي the length of the lag phase is
+
+142
+00:10:58,550 --> 00:11:03,010
+apparently dependent on a wide variety of factors
+
+143
+00:11:03,010 --> 00:11:08,210
+including شو العوامل اللي بتأثر على طول ال lag
+
+144
+00:11:08,210 --> 00:11:13,810
+phase ممكن مثلا في بكتيريا يكون ال lag phase50 أو
+
+145
+00:11:13,810 --> 00:11:18,850
+زي ما ملاحظ فيها الصورة يعني تقريباً 20 minute في
+
+146
+00:11:18,850 --> 00:11:22,490
+بكتيريا تانية ممكن يكون مثلاً خمسين minute أو
+
+147
+00:11:22,490 --> 00:11:27,350
+تلاتين minute فبيختلف طول ال lag phase من بكتيريا
+
+148
+00:11:27,350 --> 00:11:31,430
+لبكتيريا فيلم دي كتير عوامل أو عمامل بتأثر على
+
+149
+00:11:31,430 --> 00:11:35,950
+اللي هو طول ال lag phase طول مدة ال lag phase هلأ
+
+150
+00:11:35,950 --> 00:11:39,820
+بالنسبة لل generation timeالـ generation time لكل
+
+151
+00:11:39,820 --> 00:11:45,880
+بكتيريا مختلف مش نفس الشيء في كل البكتيريات طبعا
+
+152
+00:11:45,880 --> 00:11:50,100
+احنا ذكرنا لما شرحنا ال acid fast stain اللي هي
+
+153
+00:11:50,100 --> 00:11:53,760
+البكتيريا ال micro bacterium tuberculosis بتحتاج
+
+154
+00:11:53,760 --> 00:12:00,200
+20 ساعة عشان تنمو في نوع بكتيريا اخر خلال مثلا ربع
+
+155
+00:12:00,200 --> 00:12:04,080
+ساعة تلت ساعة بتنمو البكتيريا فبالتالي ال
+
+156
+00:12:04,080 --> 00:12:07,100
+generation time الوقت اللي لازم لنمو البكتيريا
+
+157
+00:12:07,100 --> 00:12:12,040
+بيختلفمن بكتيريا لأخرى ال lag phase نفس الشيء مش
+
+158
+00:12:12,040 --> 00:12:15,920
+كل البكتيريا بتتكيف بنفس الدرجة في عندي factor
+
+159
+00:12:15,920 --> 00:12:21,520
+بتأثر على طول ال lag phase أول factor the size of
+
+160
+00:12:21,520 --> 00:12:26,900
+the inicula اللي هو قداش أنا أخدت بكتيريا وزرعتها
+
+161
+00:12:26,900 --> 00:12:30,940
+في ال growth media حجم ال iniculaطبعا أكيد
+
+162
+00:12:30,940 --> 00:12:34,540
+البكتيريا الأليلة هتحضر حالها بشكل أسرع لكن
+
+163
+00:12:34,540 --> 00:12:39,160
+البكتيريا لو أنا أخدت انيكولام وكان الحجم أكتر رح
+
+164
+00:12:39,160 --> 00:12:43,540
+تاخد وقت أكتر لكن البكتيريا لو أنا أخدت انيكولام
+
+165
+00:12:43,540 --> 00:12:49,060
+وكان الحجم أليل أكيد رح تحضر حالها بشكل أسرع يبقى
+
+166
+00:12:49,060 --> 00:12:53,740
+هذا بالنسبة لأول factor بيأثر على ال lag fast تاني
+
+167
+00:12:53,740 --> 00:12:59,110
+factor timeالذي يجب أن يتجاوز من المصادر الواقعية
+
+168
+00:12:59,110 --> 00:13:05,050
+أو من المصادر الواقعية بقول لي الوقت اللازم حتى أن
+
+169
+00:13:05,050 --> 00:13:08,790
+البكتيريا تتكيف في حال صار في عندها صدمة خلال
+
+170
+00:13:08,790 --> 00:13:13,790
+عملية النقل البكتيريا قبل ما أنا أزرعها في اللي هو
+
+171
+00:13:13,790 --> 00:13:16,990
+ال media أكيد بتكون في وسط ممكن تكون في تربة في
+
+172
+00:13:16,990 --> 00:13:21,690
+هواء في طعام فبالتالي خلال ال transfer ممكن تكون
+
+173
+00:13:21,690 --> 00:13:26,620
+اتعرضت ل shock ل physical damageالوقت اللي بتحتاجه
+
+174
+00:13:26,620 --> 00:13:30,660
+هذه البكتيريا عشان تتكيف مع الوسط اللي أنا وضعته
+
+175
+00:13:30,660 --> 00:13:34,100
+فيها مع ال media اللي أنا وضعتها فيها أو تعالج
+
+176
+00:13:34,100 --> 00:13:38,700
+نفسها من الأضرار واصضاماتها ده كله بيأثر على اللي
+
+177
+00:13:38,700 --> 00:13:43,720
+هو الطول ال lag fest تالت factor time required for
+
+178
+00:13:43,720 --> 00:13:48,360
+synthesis of essential coenzyme or division factor
+
+179
+00:13:48,580 --> 00:13:52,920
+الوقت اللازم إنها اتصنع الـ Coenzyme شو هو الـ
+
+180
+00:13:52,920 --> 00:13:56,560
+Coenzyme؟ هو الفاكتور اللي بيساعد على عمل الانزيم
+
+181
+00:13:56,560 --> 00:14:02,220
+كمحفزة تآخر فاكتور بيأثر على طول الـ lag phase
+
+182
+00:14:02,220 --> 00:14:06,260
+اللي هو الـ time required for synthesis of a new
+
+183
+00:14:06,260 --> 00:14:09,480
+enzyme that are necessary to metabolize the
+
+184
+00:14:09,480 --> 00:14:13,560
+substrate present in the media هو الوقت اللازم
+
+185
+00:14:13,560 --> 00:14:18,020
+لإنتاج انزيماتضرورية عشان يصير في هندي اللي هو
+
+186
+00:14:18,020 --> 00:14:22,280
+تساعدها أو تستعملها في العمليات الحيوية في الـ
+
+187
+00:14:22,280 --> 00:14:26,780
+metabolism و تشتغل على ال substrate الموجود في ال
+
+188
+00:14:26,780 --> 00:14:31,340
+media هذا بالنسبة لل factor اللي بيأثر على طول ال
+
+189
+00:14:31,340 --> 00:14:36,820
+lag phase ويقولنا إن ال lag phase اللي هو بيختلفمش
+
+190
+00:14:36,820 --> 00:14:40,640
+كل البكتيريا بتتكيف بنفس الدرجة نفس فكرة ال
+
+191
+00:14:40,640 --> 00:14:43,540
+generation type انه لكل بكتيريا بيكون فيها انديه
+
+192
+00:14:43,540 --> 00:14:49,040
+generation type مختلف عن البكتيريا الأخرى هذا
+
+193
+00:14:49,040 --> 00:14:52,200
+بالنسبة لهذا ال slide
+
+194
+00:14:57,950 --> 00:15:02,270
+بعد ما البكتيريا اتأقلمت وحضرت حالها راح تبدأ
+
+195
+00:15:02,270 --> 00:15:05,770
+بالانقسام بالـ cell division يعني هيصير في عندي
+
+196
+00:15:05,770 --> 00:15:11,050
+نمو وزيادة بالعدد بالـ number وهي مرحلة الـ log
+
+197
+00:15:11,050 --> 00:15:15,010
+phase أو الـ X potential phase الـ X potential
+
+198
+00:15:15,010 --> 00:15:19,490
+phase of growth is a pattern of balance growth ليش
+
+199
+00:15:19,490 --> 00:15:23,130
+قالي balance growth؟ لأن البكتيريا راح تبدأ تتضاعف
+
+200
+00:15:23,130 --> 00:15:27,740
+بمعدل ثابتيعني كل خمس دقايق على سبيل المثال لها
+
+201
+00:15:27,740 --> 00:15:32,440
+تتضعف البكتيريا الواحدة وتصير اتناء طبعا هذا بسميه
+
+202
+00:15:32,440 --> 00:15:37,660
+اللي هو الزيادة اللغارتمية or الأسية growing by
+
+203
+00:15:37,660 --> 00:15:43,380
+geometric progression هذا بالنسبة لـ Geometric
+
+204
+00:15:43,380 --> 00:15:48,080
+progression هو الزيادة الأسية واللغارتمية أن
+
+205
+00:15:48,080 --> 00:15:51,960
+البكتيريا بتتضعف بمعدل ثابت كل خمس دقايق بتتضعف
+
+206
+00:15:52,220 --> 00:15:55,200
+بتصير على سبيل المثال طبعا هي مش كل البكتيريا
+
+207
+00:15:55,200 --> 00:15:58,900
+بيكون كل خمس دقايق لأ بيختلف من البكتيريا لآخر على
+
+208
+00:15:58,900 --> 00:16:03,060
+سبيل المثال انه في كل خمس دقائق بتتضاعف وبتصير
+
+209
+00:16:03,060 --> 00:16:06,660
+اتنين ممكن بكتيريا أخرى كل عشر دقائق بتتضاعف
+
+210
+00:16:06,660 --> 00:16:11,300
+وبتصير اتنين لكن بالنهاية التضاعف بيصير بمعدل ثابت
+
+211
+00:16:11,300 --> 00:16:16,900
+بيكون زيادة ليجوريتمية أو أستية wherein all the
+
+212
+00:16:16,900 --> 00:16:21,490
+cells are dividingregularly by binary illusion
+
+213
+00:16:21,490 --> 00:16:25,290
+طبعا الخلايا تنقسم بشكل منتظم زي ما قلنا بمعدل
+
+214
+00:16:25,290 --> 00:16:31,490
+ثابت بالانشطار الثنائي the cells divide at a
+
+215
+00:16:31,490 --> 00:16:36,110
+constant rate قلنا ان الخلايا تنقسم بمعدل ثابت
+
+216
+00:16:36,110 --> 00:16:40,430
+وفهمنا شو يعني بمعدل ثابتبالتالي، يعتمد على
+
+217
+00:16:40,430 --> 00:16:48,110
+محتويات الميديا وضروف الانكيباشيا طبعا، النمو
+
+218
+00:16:48,110 --> 00:16:53,650
+يعتمد على محتويات الميديا وكذلك على ضروف
+
+219
+00:16:53,650 --> 00:16:58,790
+الانكيباشيا قيمة إكس بوتنشيل إكروث بكتيريال
+
+220
+00:16:58,790 --> 00:17:04,210
+كالتشارت إكسبرس كجنراشن تايم الجنراشن تايم اللي
+
+221
+00:17:04,210 --> 00:17:09,120
+عرفناه في أول المحاضرة وقلناه هو الوقت اللازملأن
+
+222
+00:17:09,120 --> 00:17:15,380
+البكتيريا تتضاعف هو نفس الـ X potential growth of
+
+223
+00:17:15,380 --> 00:17:20,720
+bacterial culture يبقى لو أنا حكيت generation time
+
+224
+00:17:20,720 --> 00:17:25,320
+يبقى أنا بعبر عن اللي هو الـ X potential فاس برضه
+
+225
+00:17:25,320 --> 00:17:30,220
+له اسم تاني اللي هو ال doubling time لأنه قلنا أن
+
+226
+00:17:30,220 --> 00:17:35,300
+البكتيريا بيصير لها تضاعف doubling يعني تضاعف ففي
+
+227
+00:17:35,300 --> 00:17:45,040
+هذاالفاس البكتيريا بتتضعف بمعدل ثابت بالنسبة
+
+228
+00:17:45,040 --> 00:17:50,520
+لتالت مرحلة اللي هي الستشانري فاس البكتيريا لما
+
+229
+00:17:50,520 --> 00:17:54,220
+تموت بتنتج فضلات بتنتج waste بعدها رح تبدأ
+
+230
+00:17:54,220 --> 00:17:58,600
+البكتيريا تموت لما أوصل لمرحلة أنه البكتيريا أو
+
+231
+00:17:58,600 --> 00:18:02,120
+عدد البكتيريا اللي بتنمو بسوء عدد البكتيريا اللي
+
+232
+00:18:02,120 --> 00:18:07,580
+بتموتبسمي هذه المرحلة مرحلة الثبات الاستشانري فاس
+
+233
+00:18:07,580 --> 00:18:12,400
+طبعا ال rate of growth معدل النمو في مرحلة الثبات
+
+234
+00:18:12,400 --> 00:18:17,020
+بيساوي سفر exponential growth cannot be continue
+
+235
+00:18:17,020 --> 00:18:20,840
+forever in a batch culture for example closed
+
+236
+00:18:20,840 --> 00:18:25,340
+system such as destitute or افلسف بقولي انه في ال
+
+237
+00:18:25,340 --> 00:18:30,140
+exponential growth ما بيستمر في ال batch culture
+
+238
+00:18:30,510 --> 00:18:34,470
+واتفقنا على هذه المعلومة لما شرحنا شو هي الـ Batch
+
+239
+00:18:34,470 --> 00:18:38,350
+Culture الـ Batch Culture قولنا هي نظام مغلق ما
+
+240
+00:18:38,350 --> 00:18:42,810
+بضيف مواد غذائية بعد الـ Inclusion فبالتالي لما
+
+241
+00:18:42,810 --> 00:18:47,490
+توصل البكتيريا لمرحلة أو لما ال media يصير فيها
+
+242
+00:18:47,490 --> 00:18:51,410
+نقص في المواد الغذائية تخلص المواد الغذائية أكيد
+
+243
+00:18:51,410 --> 00:18:58,490
+البكتيريا هتبدأ تموت فبالتاليهنا هوصل لمرحلة اللي
+
+244
+00:18:58,490 --> 00:19:02,470
+هو الـ stationary phase ومرحلة الـ death phase الـ
+
+245
+00:19:02,470 --> 00:19:07,190
+X potential إجراث مش هيظل مستمر لكن هذا بالنسبة لل
+
+246
+00:19:07,190 --> 00:19:10,570
+batch culture لكن في ال continuous culture اللي
+
+247
+00:19:10,570 --> 00:19:14,450
+قلنا أنا بضيف مواد غذائية فبالتالي الـ X potential
+
+248
+00:19:14,450 --> 00:19:19,810
+إجراث هيظل مستمر في ال continuous cultureبيكون
+
+249
+00:19:19,810 --> 00:19:23,330
+مافي عندي مرحلة اللي هو الـ death phase في الـ
+
+250
+00:19:23,330 --> 00:19:26,830
+continuous culture في الـ open system راح توقف لإن
+
+251
+00:19:26,830 --> 00:19:30,710
+مرحلة الثبات وبعدها راح أضيف مقذيات وترجع تلوم
+
+252
+00:19:30,710 --> 00:19:35,450
+بقوللي إنه في مرحلة الثبات قداش راح تبقى فيه
+
+253
+00:19:35,450 --> 00:19:38,650
+بكتيريا بيعتمد على عدة عوامل population growth is
+
+254
+00:19:38,650 --> 00:19:42,230
+limited by one of the three factors أول عامل اللي
+
+255
+00:19:42,230 --> 00:19:46,390
+هو استهلاك المواد الغذائية هل سيكون عندي نقص في
+
+256
+00:19:46,390 --> 00:19:53,030
+المقذيات؟تاني عمل اللي هو زيادة ال West زيادة ال
+
+257
+00:19:53,030 --> 00:19:56,730
+West نتيجة العمليات الأيضية و تراجعه ما هذا أكيد
+
+258
+00:19:56,730 --> 00:20:00,910
+هيأثر في اللي هو قداش راح تظل البكتيريا في
+
+259
+00:20:00,910 --> 00:20:07,330
+البكتيريا طبعا حية في Lithium آخر factor اللي هو
+
+260
+00:20:07,330 --> 00:20:12,570
+نقص المساحة نقص المساحة في الأنبوب نتيجة زيادةعدد
+
+261
+00:20:12,570 --> 00:20:16,390
+الخلايا يبطل فيها انديوسع اللي هو a lack of
+
+262
+00:20:16,390 --> 00:20:21,010
+biological space اللي هو نقص المساحة الحيوية بيبطل
+
+263
+00:20:21,010 --> 00:20:25,810
+فيها انديوسع فبالتالي هاي كل العوامل اللي بتأثر في
+
+264
+00:20:25,810 --> 00:20:30,150
+مرحلة ثبات أداش راح تبقى في بكتيريا بيتمد على هاي
+
+265
+00:20:30,150 --> 00:20:35,010
+العوامل بالنسبة لرابع مرحلة اللي هي ال death phase
+
+266
+00:20:35,010 --> 00:20:38,450
+if incubation continues after the population
+
+267
+00:20:38,450 --> 00:20:42,380
+reaches stationary phase قولي لوالـ Incubation ظل
+
+268
+00:20:42,380 --> 00:20:45,540
+مستمر بعد ما أوصل لمرحلة الـ stationary phase اللي
+
+269
+00:20:45,540 --> 00:20:49,760
+هو معدل إنه بيكون فيها ثابت a death phase follows
+
+270
+00:20:49,760 --> 00:20:55,040
+بعدها بتبدأ مرحلة الـ death phase in which the
+
+271
+00:20:55,040 --> 00:20:58,960
+viable cell population decline بقوللي إنه عدد
+
+272
+00:20:58,960 --> 00:21:02,260
+الخلايا اللي بتنمو في الـ death phase ها بتبدأ
+
+273
+00:21:02,260 --> 00:21:05,760
+اتقل في مرحلة الـ stationary قولنا إن الـ viable
+
+274
+00:21:05,760 --> 00:21:09,820
+cell بتساوي اللي هي الخلايا الحية بتساوي الخلايا
+
+275
+00:21:09,820 --> 00:21:13,820
+الميتةلكن في الـ death face الـ viable cells
+
+276
+00:21:13,820 --> 00:21:18,520
+الخلايا الحية بتبدأ اتقل هندقلي ملاحظة أنا قولتها
+
+277
+00:21:18,520 --> 00:21:22,880
+في chapter الـ Serial Dilution if counting by
+
+278
+00:21:22,880 --> 00:21:25,580
+terpidometric measurement اللي هو الـ
+
+279
+00:21:25,580 --> 00:21:30,560
+spectrophotometer or microscopic count the death
+
+280
+00:21:30,560 --> 00:21:34,600
+face cannot be observed قولنا إنه أنا ما بميز
+
+281
+00:21:34,600 --> 00:21:37,780
+الخلايا الحية والخلايا الميتة لو أنا عديت في ال
+
+282
+00:21:37,780 --> 00:21:41,600
+terpidometric measurementأو لو استخدمت الـ
+
+283
+00:21:41,600 --> 00:21:44,900
+Microscopic Count لو استخدمت الـ Microscope ما
+
+284
+00:21:44,900 --> 00:21:49,160
+بفرق بين الخلايا الحية والخلايا الميتة فقط في
+
+285
+00:21:49,160 --> 00:21:54,440
+طريقة اللي هي الـ Viable Cells اللي هي الـ Blood
+
+286
+00:21:54,440 --> 00:21:58,340
+Count هي اللي بميز الخلايا الحية والميتة هي اللي
+
+287
+00:21:58,340 --> 00:22:02,400
+بتعد فقط الـ Viable Cell ما بتعد الخلايا الميتة
+
+288
+00:22:02,400 --> 00:22:07,340
+لأنه الأكيد البكتيريا اللي ماتت مش هتنمو على الطبق
+
+289
+00:22:08,010 --> 00:22:11,150
+فبالتالي لو أنا استخدمت هدول الطريقتين مش هلاحظ
+
+290
+00:22:11,150 --> 00:22:15,210
+الـ death phase فقط في اللي هو الـ blood count هذه
+
+291
+00:22:15,210 --> 00:22:18,110
+الملاحظة طبعا و أنا ذكرتها في الـ cereal dilution
+
+292
+00:22:18,110 --> 00:22:22,450
+هنكمل during the death phase the number of viable
+
+293
+00:22:22,450 --> 00:22:27,630
+cell degrees قولنا إنه في الـ death phase الخلايا
+
+294
+00:22:27,630 --> 00:22:32,750
+الحية بتبدأ تقل بشكل اللي هو logarithmicتمام نفس
+
+295
+00:22:32,750 --> 00:22:36,170
+اللي هو في الـ X potential الـ growth قلنا بيبدأ
+
+296
+00:22:36,170 --> 00:22:40,610
+يزيد عدد الخلايا بشكل لغرتمي بشكل قصي لكن هان
+
+297
+00:22:40,610 --> 00:22:45,790
+بيبدأ يقل بشكل لغرتمي essentially the reverse of
+
+298
+00:22:45,790 --> 00:22:49,070
+the growth during the log phase فقلي بيكون في شكل
+
+299
+00:22:49,070 --> 00:22:53,010
+عكسي لل log phase لأن ال log phase بيزيد بشكل
+
+300
+00:22:53,010 --> 00:22:58,410
+لغرتمي لكن بالنسبة لل death phase بيقل بشكل لغرتمي
+
+301
+00:22:58,820 --> 00:23:04,900
+هكذا خلصنا مراحل نمو البكتيريا وشرحنا كل الـ stage
+
+302
+00:23:04,900 --> 00:23:10,160
+اللي بتمر فيها البكتيريا من مرحلة الـ lag phase
+
+303
+00:23:10,160 --> 00:23:13,520
+الـ log phase الـ stationary phase و آخر الشيء
+
+304
+00:23:13,520 --> 00:23:17,140
+اللي هو الـ death phase طبعا هذا كله في ال batch
+
+305
+00:23:17,140 --> 00:23:21,660
+culture هاني
+
+306
+00:23:21,660 --> 00:23:26,190
+موضح مراحل نمو البكتيريا في صورةقلنا في عندي أربع
+
+307
+00:23:26,190 --> 00:23:30,010
+Stages اللي هو الـ Lag phase اللي هو اللي أنا من
+
+308
+00:23:30,010 --> 00:23:34,810
+وقت ما زرعت اللي هو الانيكولام في الـ media في الـ
+
+309
+00:23:34,810 --> 00:23:39,310
+fresh media بعدها هتبدأ الخلايا تنمو يصير في عندي
+
+310
+00:23:39,310 --> 00:23:43,150
+viable cell اللي هي الخلايا الحية في الـ X
+
+311
+00:23:43,150 --> 00:23:47,710
+potential phase بتبدأ يزيد عدد الخلايا الحية في
+
+312
+00:23:47,710 --> 00:23:50,950
+الـ Stationary phase قلنا إنه بتبدأ الخلايا هان
+
+313
+00:23:50,950 --> 00:23:53,730
+تتموت بنلاحظ أنه هان في عندي اللون الزهري بدل على
+
+314
+00:23:54,220 --> 00:23:57,620
+الـ death cells الخلايا اللي بتتموت بتبدأ الخلايا
+
+315
+00:23:57,620 --> 00:24:02,300
+انها تموت بكون عدد الخلايا اللي بتنمو بساوي عدد
+
+316
+00:24:02,300 --> 00:24:05,980
+الخلايا اللي بتموت في مرحلة الـ stationary phase
+
+317
+00:24:05,980 --> 00:24:10,940
+بعدين في مرحلة الـ death phase اللي هي بتبدأ اللي
+
+318
+00:24:10,940 --> 00:24:17,740
+هو عدد الخلايا اللي حية بيبدأ يقل بشكل لغرتمي هذا
+
+319
+00:24:17,740 --> 00:24:20,480
+بالنسبة لمراحل نمو البكتيريا
+
+320
+00:24:23,210 --> 00:24:26,930
+growth in continuous culture a continuous culture
+
+321
+00:24:26,930 --> 00:24:30,470
+is an open system اتفقنا إن ال continuous culture
+
+322
+00:24:30,470 --> 00:24:34,510
+هي عبارة عن نظام مفتوح على عكس ال batch culture
+
+323
+00:24:34,510 --> 00:24:37,730
+اللي كانت نظام مغلق in which fresh media is
+
+324
+00:24:37,730 --> 00:24:40,410
+continuously added to the culture at a constant
+
+325
+00:24:40,410 --> 00:24:44,450
+rate and all the broth is removed at the same rate
+
+326
+00:24:44,450 --> 00:24:48,870
+إيش قصده في هذه الجملة إنه بنفس الوقت اللي أنا بدي
+
+327
+00:24:48,870 --> 00:24:54,450
+أضيف فيه مغذياتنفس هذا الوقت هسحب الفضلات يبقى
+
+328
+00:24:54,450 --> 00:24:57,870
+المواد الغذائية اللى انا بدي اضيفها ل ال culture
+
+329
+00:24:57,870 --> 00:25:01,150
+لأن احنا اتفقنا ان ال continuous culture بضيف فيها
+
+330
+00:25:01,150 --> 00:25:05,190
+مواد غذائية بعد ال inculation ممكن اضيف مواد
+
+331
+00:25:05,190 --> 00:25:08,430
+غذائية لما اتخلص المواد الغذائية ممكن ان انا اضيف
+
+332
+00:25:08,430 --> 00:25:12,690
+مواد غذائية بعد ال inculation فبالتالي المواد
+
+333
+00:25:12,690 --> 00:25:17,230
+الغذائية اللى انا ضفتها هتكون بنفس الوقت اللى انا
+
+334
+00:25:17,230 --> 00:25:21,570
+بدي اسحب فيه الفضلات هيكون تنين بنفس ال rateكيف
+
+335
+00:25:21,570 --> 00:25:26,490
+بدي أسحب بقولي هذه العملية بستخدم جهاز اسمه الـ
+
+336
+00:25:26,490 --> 00:25:32,170
+Chemostatic هذا مهم هذا الجهاز اللي انا بدي اضيف
+
+337
+00:25:32,170 --> 00:25:35,510
+فيه اللي هو اللي بنفس الوقت اللي بدي اضيف فيه
+
+338
+00:25:35,510 --> 00:25:40,530
+مغاديات هسحب فضلات نفس الوقت بنفس الراتب نفس
+
+339
+00:25:40,530 --> 00:25:44,470
+المعدلالتركيز في الـ cell سيصل إلى مستوى
+
+340
+00:25:44,470 --> 00:25:48,290
+ايقاليبريام يبقى مستوى مستوى مستوى مستوى مستوى
+
+341
+00:25:48,290 --> 00:25:49,450
+مستوى مستوى مستوى مستوى مستوى مستوى مستوى مستوى
+
+342
+00:25:49,450 --> 00:25:50,070
+مستوى مستوى مستوى مستوى مستوى مستوى مستوى مستوى
+
+343
+00:25:50,070 --> 00:25:51,390
+مستوى مستوى مستوى مستوى مستوى مستوى مستوى مستوى
+
+344
+00:25:51,390 --> 00:25:52,150
+مستوى مستوى مستوى مستوى مستوى مستوى مستوى مستوى
+
+345
+00:25:52,150 --> 00:25:59,430
+مستوى مستوى مستوى مستوى مستوى مستوى مستوى مستوى
+
+346
+00:25:59,430 --> 00:26:08,810
+مستوى مستوى مستوى مستو
+
+347
+00:26:09,020 --> 00:26:14,820
+في وقت معين كل وقت معين بيتضاعف اللي هو العدد
+
+348
+00:26:14,820 --> 00:26:21,280
+الخلايا البكتيرية بمعدل ثابت ال
+
+349
+00:26:21,280 --> 00:26:25,360
+factor اللي بتأثر على النمو بشكل عام في ال slides
+
+350
+00:26:25,360 --> 00:26:29,720
+القبل كان في عوامل بتأثر على مثلا ال log phase على
+
+351
+00:26:29,720 --> 00:26:32,980
+ال lag phase على ال stationary phase لكن هنا
+
+352
+00:26:32,980 --> 00:26:36,880
+بنتكلم على النمو بشكل عام أول شئ درجة الحرارة أكيد
+
+353
+00:26:36,880 --> 00:26:40,630
+في بكتيريابتنمو على درجات حرارة عالية فى بكتيريا
+
+354
+00:26:40,630 --> 00:26:44,610
+بتنمو على درجات حرارة منخفضة معظم البكتيريا بتنمو
+
+355
+00:26:44,610 --> 00:26:49,370
+على درجات حرارة متوسطة ليها الـ 37 درجة سليسيا الـ
+
+356
+00:26:49,370 --> 00:26:54,550
+pH كمان الـ pH عندي طبعا بيختلف من بكتيريا
+
+357
+00:26:54,550 --> 00:26:58,070
+لبكتيريا فى بكتيريا بتنمو at alkaline pH على سبيل
+
+358
+00:26:58,070 --> 00:27:01,690
+الميثال الـ Vibrio cholera فى اللى هو الـ neutral
+
+359
+00:27:01,690 --> 00:27:06,930
+pH وفي الـ acidic pH فبيختلف من بكتيريا لأخرىتالي
+
+360
+00:27:06,930 --> 00:27:10,610
+تعامل الـ Salt Concentration أكيد في بكتيريا
+
+361
+00:27:10,610 --> 00:27:15,710
+بتتحمل الملوحة العالية بتتحمل 7.5% NACL زي ما
+
+362
+00:27:15,710 --> 00:27:21,390
+ذكرنا اللي هي عائلة الستافيلوكوكاس تمام؟ في بتتحمل
+
+363
+00:27:21,390 --> 00:27:26,550
+درجة 6.5% من اللي هو 6.5% NACL زي الـ Enterobacter
+
+364
+00:27:26,550 --> 00:27:32,310
+لكن باقي أنواع البكتيريا اللي هي بتتحمل 85% من 100
+
+365
+00:27:32,310 --> 00:27:36,710
+% NACLالاخر الشيء الـ Oxygen Concentration وهذا
+
+366
+00:27:36,710 --> 00:27:42,110
+شرحناه بـ Chapter كامل اتكلمنا عن كل التفاصيل اللي
+
+367
+00:27:42,110 --> 00:27:49,890
+هو في Chapter الـ Oxygen Requirement في
+
+368
+00:27:49,890 --> 00:27:53,490
+هاي الـ Slide هنتكلم كيف بدنا انقيز الـ Microbial
+
+369
+00:27:53,490 --> 00:27:57,970
+Growth كيف بدي اقيس مدى النمو راح اشوف عكارة هيكون
+
+370
+00:27:57,970 --> 00:28:03,000
+فيها دي Terbidity عكارةرح أقيصها من خلال جهاز
+
+371
+00:28:03,000 --> 00:28:07,440
+Spectrophotometer هقرأ الـ Absorbance طبعاً هيكون
+
+372
+00:28:07,440 --> 00:28:10,400
+في عندي Light Source هيمر الدوء عبر اللي هي الـ
+
+373
+00:28:10,400 --> 00:28:14,500
+Sample هيكون في عندي Turbidity عكارة هيصير في عندي
+
+374
+00:28:14,500 --> 00:28:19,260
+انتصاص اللي هو الـ Absorbance و بالنهاية هقرأ الـ
+
+375
+00:28:19,260 --> 00:28:24,580
+Absorbance لكل اللي هي في خلال كل وقت معني
+
+376
+00:28:28,270 --> 00:28:31,950
+بالنسبة للأدوات اللي هستخدمها في هذه التجربة أول
+
+377
+00:28:31,950 --> 00:28:35,630
+شي اللي هي لـ Tryptic-Cypros هاي ميديا سائلة هيكون
+
+378
+00:28:35,630 --> 00:28:40,690
+مزرعة فيها Non-pathogenic E.coli هتكون كـ Stock
+
+379
+00:28:40,690 --> 00:28:45,210
+culture عشان أخد منها انيكولام E.coli وأزرع في
+
+380
+00:28:45,210 --> 00:28:50,230
+ميديا أخرى انيكولات انجلوب عشان أزرع البكتيريا من
+
+381
+00:28:50,230 --> 00:28:54,950
+الـ Stock culture Spectrophotometer عشان أقرأ الـ
+
+382
+00:28:54,950 --> 00:28:59,040
+Absorbanceتست الـ tube راك بدي راك عشان أحط ال
+
+383
+00:28:59,040 --> 00:29:06,380
+tube فيه two tube of a tryptic soy broth واحدة
+
+384
+00:29:06,380 --> 00:29:11,980
+هتكون ل البلانك عشان أصفر عليها التانية عشان أزرع
+
+385
+00:29:11,980 --> 00:29:16,480
+فيها البكتيريا لال test هاخد من اللي هي ال stock
+
+386
+00:29:16,480 --> 00:29:21,200
+culture اللي موجود فيها E.coli و هزرع في tube أخرى
+
+387
+00:29:21,200 --> 00:29:24,020
+tryptic soy broth اللي هتكون مش وجود فيها
+
+388
+00:29:24,020 --> 00:29:27,890
+البكتيرياأكيد هيكون موجود في عندي أو بيلزمني
+
+389
+00:29:27,890 --> 00:29:32,150
+incubator عشان هنمي البكتيريا اللي عندي أنا
+
+390
+00:29:32,150 --> 00:29:35,570
+بالنهاية بدي أشوف الميكروبيا الإجراث معدل انمو
+
+391
+00:29:35,570 --> 00:29:41,050
+بنسيت بارنر بما أني هزرع بكتيريا فأكيد لازم أزرع
+
+392
+00:29:41,050 --> 00:29:46,250
+في ظروف sterile surface disinfectant هذا بالنسبة
+
+393
+00:29:46,250 --> 00:29:48,790
+للأدوات اللي هستخدمها في هذه التجربة
+
+394
+00:29:55,850 --> 00:29:59,530
+بالنسبة للخطوات أول شي هغسل أيدي ألبس ال gloves
+
+395
+00:29:59,530 --> 00:30:04,650
+أجهز أدواتي ومنطقة الشغل هشغل spectrophotometer و
+
+396
+00:30:04,650 --> 00:30:10,630
+أطبطه على 450 نانومتر هعمل warm up لجهاز لمدة 30
+
+397
+00:30:10,630 --> 00:30:14,570
+minute قبل ما أخد القراءة تاني شي هجيب ال two
+
+398
+00:30:14,570 --> 00:30:18,810
+tubes of tryptoxibros قلنا بدنا يكوننا two tubes
+
+399
+00:30:18,810 --> 00:30:23,920
+of tryptoxibros واحدة هتكون لل controlمش هزرع فيها
+
+400
+00:30:23,920 --> 00:30:28,560
+أي بكتيريا هتكون Uninculated فبالتالي هتكون
+
+401
+00:30:28,560 --> 00:30:32,220
+كcontrol شو هي الـ control؟ هي كل إشي بيكون فيها
+
+402
+00:30:32,220 --> 00:30:35,740
+معادة الشغل اللي أنا بدي أقرأها اللي أنا بدي أقرأه
+
+403
+00:30:35,740 --> 00:30:39,480
+هو الـ turbidity اللي جاي من البكتيريا و النموها
+
+404
+00:30:39,480 --> 00:30:44,080
+فبالتالي هان الـ control هتكون Media tryptoxide
+
+405
+00:30:44,080 --> 00:30:47,860
+broth لكن مش مزروع فيها بكتيريا فهتكون كcontrol
+
+406
+00:30:48,450 --> 00:30:51,710
+التيوب التاني هتكون اللي هي الـ sample اللي هزرع
+
+407
+00:30:51,710 --> 00:30:54,990
+فيها بكتيريا اللي هاخد الـ non-pathogenic E.coli
+
+408
+00:30:54,990 --> 00:30:59,010
+من الـ stock culture بقول لي حطي الـ control تيوب
+
+409
+00:30:59,010 --> 00:31:02,750
+في الـ sample holder of spectrophotometer شغلي
+
+410
+00:31:02,750 --> 00:31:07,320
+اللي هي الـ light control knob هتاخدي القراءةطبعا
+
+411
+00:31:07,320 --> 00:31:11,400
+القراءة هتكون إيش بالنسبة للـ Control أكيد هتكون
+
+412
+00:31:11,400 --> 00:31:16,260
+الامتصاص الـ Absorbance صفر لأن الـ Control Tube
+
+413
+00:31:16,260 --> 00:31:21,740
+مافيها أي بكتيريا هتكون Clear فبالتالي هيمر الضوء
+
+414
+00:31:21,740 --> 00:31:25,940
+احنا قولنا أنا بقرأ Terribility فبالتالي الـ
+
+415
+00:31:25,940 --> 00:31:30,060
+Control Tube هتكون عندي اللي هي Clear هيصير امتصاص
+
+416
+00:31:30,060 --> 00:31:33,140
+مش هيصير امتصاص لأنه مافي أي شيء هتكون الـ Tube
+
+417
+00:31:33,140 --> 00:31:38,180
+Clearفبالتالي الـ Absorbance هتكون 0 بالنسبة لـ
+
+418
+00:31:38,180 --> 00:31:43,040
+النافذية هتكون 100% ليش النافذية هتكون 100%؟ لأنه
+
+419
+00:31:43,040 --> 00:31:47,520
+الامتصاص سفر الضوء اللي دخل هو نفسه الضوء اللي طلع
+
+420
+00:31:47,520 --> 00:31:51,620
+فبالتالي النافذية 100% هشيل الـ Control الـ Tube
+
+421
+00:31:51,620 --> 00:31:57,460
+هيك بيكون صفرت على ال .. اللي هو صفرت الجهاز بيكون
+
+422
+00:31:57,460 --> 00:32:00,900
+الـ Spectrophotometer جاهز لقراة اللي هي الـ
+
+423
+00:32:00,900 --> 00:32:01,260
+Sample
+
+424
+00:32:05,500 --> 00:32:09,900
+بعدها بتشغلي اللى هو الـ Benson burner بتعملي اللى
+
+425
+00:32:09,900 --> 00:32:15,160
+هو sterilization للـ loop هتخليها تبرد شوية بعدها
+
+426
+00:32:15,160 --> 00:32:20,200
+هتاخدى Ecolime من ال stock culture هتزراعيها في ال
+
+427
+00:32:20,200 --> 00:32:26,880
+acid tube اللى قولنا للـ sample بعدها طبعا هتاخدى
+
+428
+00:32:26,880 --> 00:32:31,920
+اللى هتزراعيها في ال tube طبعا هتعملي تعقيم لل
+
+429
+00:32:31,920 --> 00:32:37,400
+loop بعد استخدامتههتبدأ تقرأ الـ Turbidity لـ S
+
+430
+00:32:37,400 --> 00:32:41,120
+-Tube في الـ Sample Well لـ Spectrophotometer
+
+431
+00:32:41,120 --> 00:32:45,880
+هتاخد قراءة الـ Absorbance تمام؟ بعد ما أخدت قراءة
+
+432
+00:32:45,880 --> 00:32:49,480
+الـ Absorbance هتاخد الـ Tube وتحطيها ترجعيها في
+
+433
+00:32:49,480 --> 00:32:54,520
+الـ Incubator خلال مدة معينة مثلا ممكن أخد القراءة
+
+434
+00:32:54,520 --> 00:32:59,920
+خلال مثلا مكتوب في جدول ممكن ناخد كل مثلا خمس
+
+435
+00:32:59,920 --> 00:33:04,520
+دقايق أخد قراءةأحط أخد قراءة وبعدين أحط اللي هي
+
+436
+00:33:04,520 --> 00:33:08,200
+الـ Sample في الـ Incubator خمس دقائق وبعدين أخد
+
+437
+00:33:08,200 --> 00:33:12,880
+القراءة مرتين هيكون في عندي جدول في الـ Time وجدول
+
+438
+00:33:12,880 --> 00:33:17,040
+فيه مقابله هيكون اللي هي القراءة في كل وقت الـ
+
+439
+00:33:17,040 --> 00:33:20,440
+Absorbance بعد ما أخلص طبعا هسكر جهاز الـ
+
+440
+00:33:20,440 --> 00:33:25,700
+Spectrophotometer هجهز ورقة أرسم اللي هو محور لـ
+
+441
+00:33:25,700 --> 00:33:29,400
+Absorbance اللي هي القراءات اللي أنا أخدتها مع الـ
+
+442
+00:33:29,400 --> 00:33:35,630
+Time الوقت في كلطبعا احنا قلنا بناخد كل خمس دقايق
+
+443
+00:33:35,630 --> 00:33:40,910
+فمثلا أول شئ كانت القراءة على سبيل المثال Zero
+
+444
+00:33:40,910 --> 00:33:45,310
+بعدين بعد خمس دقايق كانت القراءة ال absorbance
+
+445
+00:33:45,310 --> 00:33:49,310
+اتنين بعد خمس دقايق طبعا عند عشر دقايق يكون وقتها
+
+446
+00:33:49,310 --> 00:33:54,150
+المدة كانت تلاتة وهكذا هاخد طبعا قراءات هعمل اللي
+
+447
+00:33:54,150 --> 00:33:59,020
+هو محاور لال absorbance و ال timeبعد ما اخلص طبعا
+
+448
+00:33:59,020 --> 00:34:07,240
+هنظف المكان و هشلح لي gloves و اغسل ايدايا هبدأ
+
+449
+00:34:07,240 --> 00:34:12,120
+اسجل اللي هو ال absorbance لكل وقت معين حسب الجدول
+
+450
+00:34:12,120 --> 00:34:16,110
+مناللاحظ انهعند كل وقت معين هيكون فيه قيمة
+
+451
+00:34:16,110 --> 00:34:21,110
+Absorbance مختلفة هعمل بالنهاية XY Axis هيكون لأ
+
+452
+00:34:21,110 --> 00:34:24,630
+لـ Absorbance و لـ Time هعمل الـ Chart بالنهاية
+
+453
+00:34:24,630 --> 00:34:28,550
+هيوضح اللي هو الـ Growth Curve الـ Lag Phase الـ X
+
+454
+00:34:28,550 --> 00:34:32,930
+Potential Phase والستشانري فازليش متذكر الـ Death
+
+455
+00:34:32,930 --> 00:34:35,610
+Face؟ لأنه إحنا قولنا في الـ Terpidometric
+
+456
+00:34:35,610 --> 00:34:40,770
+Measurement ما بقدر ألاحظ الـ Death Face لأن الـ
+
+457
+00:34:40,770 --> 00:34:44,450
+Count بيكون لـ الـ Death Cell و الـ Viable Cell مش
+
+458
+00:34:44,450 --> 00:34:47,770
+هقدر أميزهم عن بعض في الـ Microscopic Count و في
+
+459
+00:34:47,770 --> 00:34:51,370
+الـ Terpidometric Measurement لكن في الـ Black
+
+460
+00:34:51,370 --> 00:34:53,170
+Count بقدر أميز
+
+461
+00:34:58,830 --> 00:35:04,370
+هذا الـ curve موضح المراحل النمو فيه أو نستاج فيه
+
+462
+00:35:04,370 --> 00:35:10,650
+صورة يبدو أن هذا صراحة أفضل من ال curve اللي مار
+
+463
+00:35:10,650 --> 00:35:15,150
+معناه أول شيء في ال lag phase مافي عندي خلايا في
+
+464
+00:35:15,150 --> 00:35:19,030
+ال log phase اللي هو في عندي عدد الخلايا الحية
+
+465
+00:35:19,030 --> 00:35:22,970
+أكتر من عدد الخلايا الميتة إذا مانلاحظ تحت مكتوب
+
+466
+00:35:22,970 --> 00:35:27,030
+أن ال death cell بكون لونها أسود ال viable cellفي
+
+467
+00:35:27,030 --> 00:35:31,210
+ND زي نقاط كتيرة لكن في أول مرحلة بيكون في ND few
+
+468
+00:35:31,210 --> 00:35:34,550
+cells اللي هي النقاط القليلة في ال look phase
+
+469
+00:35:34,550 --> 00:35:40,710
+منلاحظ أن ال viable cell أكتر من ال death cell في
+
+470
+00:35:40,710 --> 00:35:44,290
+ال stationary phase عدد الخلايا الميتة بيساوي عدد
+
+471
+00:35:44,290 --> 00:35:48,190
+الخلايا الحية في ال death phase منلاحظ أن عدد
+
+472
+00:35:48,190 --> 00:35:54,140
+الخلايا الميتة أكتر من عدد الخلايا الحيةطبعا هذا
+
+473
+00:35:54,140 --> 00:35:57,960
+ال curve هو المظبوط أكتر من ال curve اللي مار
+
+474
+00:35:57,960 --> 00:36:03,240
+معناه لأنه موضح كل المراحل أو انبين عدد الخلايا
+
+475
+00:36:03,240 --> 00:36:04,200
+بشكل أفضل
+
diff --git a/PL9fwy3NUQKwZWgAb_V1ATlRRsH-6i3cPC/Pl7CGd4BP8U.srt b/PL9fwy3NUQKwZWgAb_V1ATlRRsH-6i3cPC/Pl7CGd4BP8U.srt
new file mode 100644
index 0000000000000000000000000000000000000000..cfc606e5baef5ee82abbbcbbe70178637adf6493
--- /dev/null
+++ b/PL9fwy3NUQKwZWgAb_V1ATlRRsH-6i3cPC/Pl7CGd4BP8U.srt
@@ -0,0 +1,2065 @@
+
+1
+00:00:01,790 --> 00:00:05,850
+السلام عليكم ورحمة الله وبركاته. نستكمل معكم
+
+2
+00:00:05,850 --> 00:00:10,670
+محاضرات مساق الميكروبيولوجي العملي. في هذا اللقاء
+
+3
+00:00:10,670 --> 00:00:15,030
+ستكون المحاضرة بعنوان: Bacteria Oxygen Requirements
+
+4
+00:00:15,030 --> 00:00:19,790
+and Anaerobic Bacteria. قلنا إن البكتيريا عشان تنمو
+
+5
+00:00:19,790 --> 00:00:25,550
+وتتكاثر، لازم أن أُوَفِّر الظروف المناسبة للنمو من
+
+6
+00:00:25,550 --> 00:00:31,040
+هذه الظروف، حاجتها للأكسجين. سنتعرف في هذه المحاضرة
+
+7
+00:00:31,040 --> 00:00:35,940
+على تصنيفات البكتيريا حسب حاجتها للأكسجين، وكيف أنا
+
+8
+00:00:35,940 --> 00:00:40,780
+أُريد أن أُوَفِّر هذه الظروف، عشان أقدر أنمي البكتيريا.
+
+9
+00:00:40,780 --> 00:00:44,460
+Introduction
+
+10
+00:00:44,460 --> 00:00:48,200
+البكتيريا can exist in a wide variety of
+
+11
+00:00:48,200 --> 00:00:51,560
+environments, including environments that lack
+
+12
+00:00:51,560 --> 00:00:56,690
+oxygen. بقول إن البكتيريا ممكن إنها تنمو في بيئات
+
+13
+00:00:56,690 --> 00:01:01,930
+مختلفة، من ضمن هذه البيئات البيئة التي ليس موجود
+
+14
+00:01:01,930 --> 00:01:06,050
+فيها أكسجين. In fact, oxygen is toxic to some
+
+15
+00:01:06,050 --> 00:01:10,330
+bacteria. بقول في الحقيقة الأكسجين يعتبر سامّاً
+
+16
+00:01:10,330 --> 00:01:16,750
+للبعض من البكتيريا. The toxicity is usually due to
+
+17
+00:01:16,750 --> 00:01:20,890
+accidental formation of superoxide and hydrogen
+
+18
+00:01:20,890 --> 00:01:24,700
+peroxide, which react with other cell components.
+
+19
+00:01:24,700 --> 00:01:29,820
+بقول إن الأكسجين سام لبعض أنواع البكتيريا.
+
+20
+00:01:29,820 --> 00:01:34,600
+قلنا إن الأكسجين هو المستقبل النهائي للإلكترونات
+
+21
+00:01:34,600 --> 00:01:40,400
+في التنفس الهوائي، طبعاً.
+
+22
+00:01:40,400 --> 00:01:45,380
+في التنفس الخلوي، يرتبط الإلكترون طبعاً فيه. أنا
+
+23
+00:01:45,380 --> 00:01:49,560
+عندي في سلسلة نقل الإلكترون،  في مضخات
+
+24
+00:01:49,560 --> 00:01:55,840
+لإلكترونات. هيرتبط الإلكترون مع اللي هو
+
+25
+00:01:55,840 --> 00:02:01,880
+الأكسجين، مع المستقبل النهائي للإلكترونات، سيتكون
+
+26
+00:02:01,880 --> 00:02:07,900
+Superoxide، سيتكون Superoxide اللي هو O2−
+
+27
+00:02:07,900 --> 00:02:14,480
+هذا مكتسب إلكترون، يعتبر هذا سامّاً، لأنه بيقول
+
+28
+00:02:14,480 --> 00:02:17,940
+يقول إن هذه السمية عادةً تكون عالية
+
+29
+00:02:17,940 --> 00:02:22,520
+نتيجة لتكون Superoxide. كيف تكون
+
+30
+00:02:22,520 --> 00:02:26,000
+Superoxide خلال عملية التنفس الهوائي؟
+
+31
+00:02:26,000 --> 00:02:32,540
+المستقبل النهائي للإلكترونات هو الأكسجين، تمام.
+
+32
+00:02:32,540 --> 00:02:36,000
+الأكسجين هو المستقبل النهائي للإلكترونات، سيرتبط
+
+33
+00:02:36,000 --> 00:02:40,320
+مع إلكترون، ويتكون Superoxide. طبعاً هذا يعتبر
+
+34
+00:02:40,320 --> 00:02:46,010
+سامّاً. يتم التخلص منه عن طريق إنزيم Superoxide
+
+35
+00:02:46,010 --> 00:02:51,350
+dismutase. تكلمنا في معمل أو فصل Catalase
+
+36
+00:02:51,350 --> 00:02:56,170
+Test، أنه أنا أتخلص من Superoxide عن طريق إنزيم
+
+37
+00:02:56,170 --> 00:03:02,830
+Superoxide dismutase، سيُحَوِّله إلى H2O2
+
+38
+00:03:02,830 --> 00:03:06,550
+الذي هو بيروكسيد الهيدروجين. كيف أنا أُريد أن أتخلص من
+
+39
+00:03:06,550 --> 00:03:10,630
+بيروكسيد الهيدروجين الذي يعتبر أيضاً سامّاً
+
+40
+00:03:10,960 --> 00:03:16,860
+يتم التخلص منه إما عن طريق إنزيم الكاتالاز أو عن
+
+41
+00:03:16,860 --> 00:03:21,480
+طريق إنزيم بيروكسيداز الهيدروجين. طبعاً، في معادلات
+
+42
+00:03:21,480 --> 00:03:27,240
+شرحناها سابقاً،  ما هي نواتج عملية تكسير بيروكسيد الهيدروجين
+
+43
+00:03:27,240 --> 00:03:31,540
+بواسطة الكاتالاز أو بواسطة إنزيم الكاتالاز، و
+
+44
+00:03:31,540 --> 00:03:35,740
+بواسطة إنزيم بيروكسيداز الهيدروجين، وسنُذْكُره في
+
+45
+00:03:35,740 --> 00:03:41,900
+الشرائح التالية. تمام، هذا بالنسبة إلى أن السمية من أين
+
+46
+00:03:41,900 --> 00:03:45,220
+جائت، من تكون Superoxide وبيروكسيد الهيدروجين.
+
+47
+00:03:45,220 --> 00:03:50,900
+بالنسبة للبكتيريا التي ليس عندها هذه
+
+48
+00:03:50,900 --> 00:03:54,180
+الإنزيمات، التي ليس عندها إنزيم Superoxide
+
+49
+00:03:54,180 --> 00:03:59,180
+dismutase أو الكاتالاز أو بيروكسيداز الهيدروجين، سيكون
+
+50
+00:03:59,180 --> 00:04:05,260
+الأكسجين بالنسبة لها سامّاً، فبالتالي لن تلجأ
+
+51
+00:04:05,260 --> 00:04:10,590
+إلى الظروف الهوائية، لن تستخدم التنفس الهوائي
+
+52
+00:04:10,590 --> 00:04:16,450
+للحصول على الطاقة، ستلجأ إلى الظروف اللاهوائية، ستلجأ إلى
+
+53
+00:04:16,450 --> 00:04:21,750
+التنفس اللاهوائي. ستستخدم جزيء غير عضوي
+
+54
+00:04:21,750 --> 00:04:27,050
+غير الأكسجين كمستقبل نهائي للإلكترونات. Oxygen species
+
+55
+00:04:27,050 --> 00:04:31,430
+is harmful if bacteria have no enzyme system to
+
+56
+00:04:31,430 --> 00:04:35,550
+eliminate it. بقول إن الأكسجين يعتبر ضاراً لو
+
+57
+00:04:35,550 --> 00:04:39,610
+البكتيريا ليس عندها الإنزيمات عشان تتخلص من
+
+58
+00:04:39,610 --> 00:04:42,890
+الأشكال السامة للأكسجين، مثل Superoxide و
+
+59
+00:04:42,890 --> 00:04:46,470
+بيروكسيد الهيدروجين. إذا لم يكن عندها إنزيم Superoxide
+
+60
+00:04:46,470 --> 00:04:50,550
+dismutase أو إنزيم الكاتالاز أو بيروكسيداز الهيدروجين، هذا سيعتبر بالنسبة لها الأكسجين
+
+61
+00:04:50,550 --> 00:04:54,490
+سامّاً. معلومة ثانية، إن الأكسجين يكون ضاراً لو كان
+
+62
+00:04:54,490 --> 00:05:00,400
+تركيز أنواع الأكسجين  عالي جداً في
+
+63
+00:05:00,400 --> 00:05:03,860
+الوسط أو الهواء. لو كانت كمية الأكسجين أكثر من الكمية
+
+64
+00:05:03,860 --> 00:05:09,480
+التي تحتاجها البكتيريا، فبالتالي سيكون هو
+
+65
+00:05:09,480 --> 00:05:13,520
+سامّاً.  بعض أنواع البكتيريا. طبعاً، الأكسجين في
+
+66
+00:05:20,200 --> 00:05:27,980
+الهواء، نسبة الأكسجين في الهواء 21%. إذا كانت نسبة
+
+67
+00:05:27,980 --> 00:05:30,860
+الأكسجين أعلى من الموجودة في الوسط أو في الهواء
+
+68
+00:05:30,860 --> 00:05:36,840
+بشكل عام، مما تحتاجه البكتيريا، يعتبر سامّاً، فبالتالي في هذه
+
+69
+00:05:36,840 --> 00:05:41,740
+الحالتين، لو البكتيريا ليس عندها إنزيمات للتخلص من
+
+70
+00:05:41,740 --> 00:05:46,470
+الأكسجين، أو من الأشكال السامة للأكسجين، أو لو كان
+
+71
+00:05:46,470 --> 00:05:50,730
+نسبة الأكسجين في الوسط أو في الهواء أعلى من حاجة
+
+72
+00:05:50,730 --> 00:05:54,430
+أو أعلى من الكمية التي تحتاجها البكتيريا، سيكون
+
+73
+00:05:54,430 --> 00:05:59,210
+سامّاً. في هذه الحالات، أكيد البكتيريا ستلجأ إلى الظروف
+
+74
+00:05:59,210 --> 00:06:05,850
+اللاهوائية للحصول على الطاقة.  طيب، نأتي إلى الأشكال
+
+75
+00:06:05,850 --> 00:06:13,310
+السامة للأكسجين. أول شكل من أشكال
+
+76
+00:06:13,310 --> 00:06:18,590
+الأكسجين التي تكون سامة،  الأكسجين الأحادي، بقول لي هو
+
+77
+00:06:18,590 --> 00:06:22,930
+في حالة طاقة عالية، شديد التفاعل، يوجد في
+
+78
+00:06:22,930 --> 00:06:27,970
+الخلايا البلعمية. بقول لي هذا يعتبر في حالة طاقة عالية،
+
+79
+00:06:27,970 --> 00:06:33,590
+يكون شديد التفاعل، نشط، يُسْتَخْدَم، يتم
+
+80
+00:06:33,590 --> 00:06:38,830
+استخدامه في البلعمة، طبعاً الخلايا
+
+81
+00:06:38,830 --> 00:06:45,330
+البلعمية، عندما تُهاجَم من ميكروب أو
+
+82
+00:06:45,330 --> 00:06:50,250
+بكتيريا وتقوم بالبلعمة، تُفْرِز عليه
+
+83
+00:06:50,250 --> 00:06:56,190
+الأكسجين الأحادي، فبالتالي يُقْتَل
+
+84
+00:06:56,190 --> 00:07:00,850
+تموت البكتيريا أو الميكروب. هذا بالنسبة لأول شكل سام
+
+85
+00:07:00,850 --> 00:07:05,210
+من الأكسجين. ثاني شكل سام، Superoxide الجذور الحرة
+
+86
+00:07:05,210 --> 00:07:09,830
+و بيروكسيد الهيدروجين، اللذان يُنتَجَان
+
+87
+00:07:09,830 --> 00:07:14,070
+كميات قليلة في التنفس الخلوي
+
+88
+00:07:14,070 --> 00:07:18,170
+اللذان يُنتَجَان في التنفس الخلوي أو في
+
+89
+00:07:18,170 --> 00:07:23,560
+التنفس الهوائي. هذان سامّان جداً للخلية، طبعاً
+
+90
+00:07:23,560 --> 00:07:28,560
+هذان يُعتَبَران سامّين، يتم التخلص منهما عن طريق، طبعاً
+
+91
+00:07:28,560 --> 00:07:32,500
+بالنسبة لـ Superoxide الجذر الحر، بواسطة
+
+92
+00:07:32,500 --> 00:07:36,980
+إنزيم Superoxide dismutase، سيتكون
+
+93
+00:07:36,980 --> 00:07:39,480
+بيروكسيد الهيدروجين. كيف نتخلص من بيروكسيد الهيدروجين؟
+
+94
+00:07:39,480 --> 00:07:44,140
+إما بواسطة إنزيم الكاتالاز أو بواسطة إنزيم
+
+95
+00:07:44,140 --> 00:07:48,500
+بيروكسيداز الهيدروجين. طبعاً، التنفس الهوائي
+
+96
+00:07:48,500 --> 00:07:55,580
+الذي هو عملية التنفس الهوائي الخلوي، بما أنها تنمو
+
+97
+00:07:55,580 --> 00:08:00,520
+في الظروف الهوائية، يجب أن يكون عندها هذه الإنزيمات
+
+98
+00:08:00,520 --> 00:08:04,820
+التي تكلمنا عنها، عشان تتخلص من الأشكال السامة
+
+99
+00:08:04,820 --> 00:08:09,520
+للأكسجين، من هذه الأشكال السامة، أيون بيروكسيد  الذي هو
+
+100
+00:08:09,520 --> 00:08:15,440
+O2−2. هذا هو Superoxide الجذر الحر، و
+
+101
+00:08:15,440 --> 00:08:20,660
+اكتسب إلكترون آخر.  Used as antimicrobial
+
+102
+00:08:20,660 --> 00:08:25,060
+agent. ماذا يعني antimicrobial agent؟ يعني مادة
+
+103
+00:08:25,060 --> 00:08:29,660
+تُسْتَخْدَم في قتل أو تثبيت البكتيريا. هذا معنى
+
+104
+00:08:29,660 --> 00:08:33,880
+المُضادّات الحيوية، التي سنتكلم عنها بالتفصيل في
+
+105
+00:08:33,880 --> 00:08:41,410
+الفصل الأخير في المساق. Hydroxyl Radical
+
+106
+00:08:41,410 --> 00:08:47,070
+الذي هو OH−. The most active form in the
+
+107
+00:08:47,070 --> 00:08:51,310
+cytoplasm from respiration. طبعاً هو الشكل الأكثر نشاطاً
+
+108
+00:08:51,310 --> 00:08:56,530
+ويكون موجود في السيتوبلازم. هذا بالنسبة للأشكال
+
+109
+00:08:56,530 --> 00:09:03,530
+السامة للأكسجين. نأتي إلى كيف أنا أُريد أن أزيل هذه
+
+110
+00:09:03,530 --> 00:09:09,780
+السمية. بقول لي:  Toxic forms of oxygen,  ممكن أن أحتاج أن
+
+111
+00:09:09,780 --> 00:09:15,840
+أحتاج أن أزيل هذه السمية، السمية لهذه
+
+112
+00:09:15,840 --> 00:09:20,320
+الأشكال السامة للأكسجين باستخدام إنزيمات. قلنا Superoxide
+
+113
+00:09:20,320 --> 00:09:26,700
+dismutase تتخلص من الجذر الحر،
+
+114
+00:09:26,700 --> 00:09:32,900
+O2−، Superoxide الجذر الحر. الكاتالاز تتخلص من
+
+115
+00:09:32,900 --> 00:09:36,320
+بيروكسيد الهيدروجين. بيروكسيداز تتخلص من
+
+116
+00:09:36,320 --> 00:09:41,580
+بيروكسيد الهيدروجين أيضاً. إذا لم يكن الميكروب ينتج
+
+117
+00:09:41,580 --> 00:09:45,540
+هذه الإنزيمات، يجب أن تكون له حالة لاهوائية. بقول
+
+118
+00:09:45,540 --> 00:09:50,320
+لو البكتيريا أو الميكروب ليس عنده هذه الإنزيمات، يجب أن
+
+119
+00:09:50,320 --> 00:09:55,420
+تلجأ البكتيريا إلى الظروف اللاهوائية للحصول على الطاقة.
+
+120
+00:09:55,420 --> 00:09:58,440
+الخلايا التي تعيش في بيئة تحتوي على أكسجين تحتوي
+
+121
+00:09:58,440 --> 00:10:05,730
+على إنزيم Superoxide dismutase والكاتالاز. Convert
+
+122
+00:10:05,730 --> 00:10:09,130
+superoxide and peroxide to oxygen and water.
+
+123
+00:10:09,130 --> 00:10:14,430
+بقول إن الخلايا التي تستطيع العيش في بيئة فيها
+
+124
+00:10:14,430 --> 00:10:18,690
+أكسجين، أكيد ستمتلك إنزيم Superoxide
+
+125
+00:10:18,690 --> 00:10:23,050
+dismutase والكاتالاز. طبعاً، ستحول Superoxide
+
+126
+00:10:23,050 --> 00:10:27,410
+و بيروكسيد الهيدروجين، هذان طبعاً الأشكال السامة
+
+127
+00:10:27,410 --> 00:10:32,880
+للأكسجين، إلى أكسجين وماء، حسب المعادلات التي
+
+128
+00:10:32,880 --> 00:10:37,960
+سنراها في الشريحة التالية. طبعاً، الأكسجين وال
+
+129
+00:10:37,960 --> 00:10:42,700
+ماء، هذان أشكال غير سامة، فبالتالي هكذا زالت سمية
+
+130
+00:10:42,700 --> 00:10:48,740
+Superoxide وبيروكسيد الهيدروجين أو
+
+131
+00:10:48,740 --> 00:10:53,680
+بيروكسيد الهيدروجين. هذا بالنسبة للمعادلات.
+
+132
+00:10:53,680 --> 00:10:57,920
+Superoxide dismutase، هذا الإنزيم يحول Superoxide
+
+133
+00:10:57,920 --> 00:11:03,790
+ions. طبعاً، قلنا Superoxide الذي هو
+
+134
+00:11:03,790 --> 00:11:07,470
+الجذر الحر، هذا يعتبر سامّاً، فبالتالي كيف
+
+135
+00:11:07,470 --> 00:11:10,250
+أنا أريد أن أتخلص منه؟  في عندي أنا في سلسلة نقل الإلكترون
+
+136
+00:11:10,250 --> 00:11:14,850
+في عندي أيون هيدروجين سيتم
+
+137
+00:11:14,850 --> 00:11:18,430
+الارتباط مع أيون الهيدروجين، باستخدام إنزيم
+
+138
+00:11:18,430 --> 00:11:23,490
+Superoxide dismutase، سيتكون عندي H2O2 + O2
+
+139
+00:11:23,490 --> 00:11:30,410
+قلنا ستكون في النهاية ماء وأكسجين. هذا H2O2
+
+140
+00:11:30,410 --> 00:11:34,630
+يُعْتَبَر شكلاً سامّاً. كيف أتخلص منه؟ إما باستخدام إنزيم
+
+141
+00:11:34,630 --> 00:11:39,250
+الكاتالاز أو باستخدام إنزيم البيروكسيداز، يكسر
+
+142
+00:11:39,250 --> 00:11:43,470
+الكاتالاز يكسر بيروكسيد الهيدروجين الذي تم إنتاجه
+
+143
+00:11:43,470 --> 00:11:47,050
+أو تم تكوينه من الجذر الحر الذي هو Superoxide
+
+144
+00:11:47,050 --> 00:11:51,690
+مع أيون الهيدروجين، سيتم تكسير بيروكسيد الهيدروجين
+
+145
+00:11:51,690 --> 00:11:56,830
+بواسطة إنزيم الكاتالاز إلى ماء وأكسجين.
+
+146
+00:11:56,830 --> 00:12:01,010
+هكذا أنا استطعت التخلص من الأشكال السامة للأكسجين.
+
+147
+00:12:02,200 --> 00:12:06,520
+بالنسبة لبيروكسيداز، نفس الكاتالاز، يعمل على
+
+148
+00:12:06,520 --> 00:12:10,140
+بيروكسيد الهيدروجين، لكن بيروكسيداز أو
+
+149
+00:12:10,140 --> 00:12:14,400
+بيروكسيداز الهيدروجين يحول أو يكسر
+
+150
+00:12:14,400 --> 00:12:20,380
+بيروكسيد الهيدروجين إلى جزيئين من الماء،
+
+151
+00:12:20,380 --> 00:12:26,400
+2H2O، يبقى في النهاية سيتكون عندي ماء وأكسجين، غير
+
+152
+00:12:26,400 --> 00:12:31,060
+سامّين. أستطيع أن أتخلص من سمية الأكسجين.
+
+153
+00:12:39,020 --> 00:12:44,060
+نأتي إلى تصنيف حاجة البكتيريا للأكسجين، تصنيف
+
+154
+00:12:44,060 --> 00:12:48,780
+البكتيريا حسب حاجتها للأكسجين.  وقلنا تم تصنيف
+
+155
+00:12:48,780 --> 00:12:54,460
+البكتيريا حسب حاجتها للأكسجين إلى خمسة تصنيفات. أول
+
+156
+00:12:54,460 --> 00:12:58,520
+تصنيف، الهوائية الإجبارية. طبعاً،  Obligate Aerobic
+
+157
+00:12:58,520 --> 00:13:04,760
+معناها هوائية إجبارية. Require oxygen to
+
+158
+00:13:04,760 --> 00:13:11,930
+live. تحتاج أو تستخدم الأكسجين حتى تستطيع العيش.
+
+159
+00:13:11,930 --> 00:13:19,890
+يبقى هي تحتاج الأكسجين حتى تستطيع العيش، تستخدمه
+
+160
+00:13:19,890 --> 00:13:26,690
+كمستقبل نهائي للإلكترونات للحصول على الطاقة. Their
+
+161
+00:13:26,690 --> 00:13:30,480
+metabolic pathway requires oxygen as a final
+
+162
+00:13:30,480 --> 00:13:33,600
+electron acceptor. خلال التنفس الهوائي،
+
+163
+00:13:33,600 --> 00:13:39,720
+يبقى هي تستخدم التنفس الهوائي للحصول على
+
+164
+00:13:39,720 --> 00:13:44,280
+الطاقة، لأن الأكسجين هو المستقبل النهائي
+
+165
+00:13:44,280 --> 00:13:48,900
+للإلكترونات. في عندي طريقة أخرى للحصول على الطاقة؟
+
+166
+00:13:48,900 --> 00:13:53,320
+بقول: They have no alternate means of producing
+
+167
+00:13:53,320 --> 00:13:56,900
+ATP. بقول: ليس لدي أي طريقة أخرى للحصول على الطاقة
+
+168
+00:13:56,900 --> 00:14:01,630
+غير التنفس الهوائي، لأنها تحتاج الأكسجين
+
+169
+00:14:01,630 --> 00:14:05,310
+حتى تستطيع العيش، فبالتالي ستستخدم الأكسجين
+
+170
+00:14:05,310 --> 00:14:13,250
+كمستقبل نهائي للإلكترونات. طيب، بما أنها هوائية، بما
+
+171
+00:14:13,250 --> 00:14:18,490
+أنها هوائية، تستخدم الأكسجين كمستقبل نهائي
+
+172
+00:14:18,490 --> 00:14:23,170
+للإلكترونات، أكيد سيكون عندها كل الإنزيمات التي
+
+173
+00:14:23,170 --> 00:14
+
+223
+00:18:30,570 --> 00:18:34,540
+هيتم استخدام ال anaerobic respiration في الـ
+
+224
+00:18:34,540 --> 00:18:38,220
+obligate anaerobic للحصول على الطاقة فقط
+
+225
+00:18:38,220 --> 00:18:43,580
+anaerobic respiration وال fermentation  طيب ليش ما
+
+226
+00:18:43,580 --> 00:18:50,620
+بتقدر تنمو في وجود ال oxygen؟ بقول لي هي بتفقد
+
+227
+00:18:50,620 --> 00:18:55,880
+الإنزيمات، هذه الإنزيمات من خلالها بنتخلص من
+
+228
+00:18:55,880 --> 00:19:00,560
+ال toxic form of oxygen لأن super oxide dismutase
+
+229
+00:19:00,560 --> 00:19:05,180
+و catalase و peroxidase، وبالتالي هتموت في وجود ال oxygen
+
+230
+00:19:05,180 --> 00:19:09,400
+يبقى ما عندها الإنزيمات للتخلص من ال toxic form of
+
+231
+00:19:09,400 --> 00:19:15,920
+oxygen، فبالتالي مش هتقدر تنمو إلا في حال عدم وجود
+
+232
+00:19:15,920 --> 00:19:20,880
+ال oxygen. لو كان موجود ال oxygen هتموت (die in the
+
+233
+00:19:20,880 --> 00:19:25,100
+presence of oxygen). دي عدم وجود الإنزيمات اللازمة
+
+234
+00:19:25,100 --> 00:19:29,540
+للتخلص من ال toxic form of oxygen. يبقى ما عندها
+
+235
+00:19:29,540 --> 00:19:36,110
+الإنزيمات اللي ذكرناها لأنها لاهوائية. طيب لو أنا
+
+236
+00:19:36,110 --> 00:19:39,890
+زرعت بكتيريا Obligate anaerobic في broth
+
+237
+00:19:39,890 --> 00:19:44,770
+tube فقط، هلاقي النمو في الأسفل، هيكون sediment لأن
+
+238
+00:19:44,770 --> 00:19:51,370
+هي بتنمو في حال عدم وجود ال oxygen. طب نيجي لرابع
+
+239
+00:19:51,370 --> 00:19:56,490
+تصنيف: ال Aerotolerant Anaerobic Bacteria. هذه
+
+240
+00:19:56,490 --> 00:20:02,550
+البكتيريا هي بالأصل لاهوائية. لما إحنا قلنا على ال
+
+241
+00:20:02,550 --> 00:20:07,010
+facultative anaerobic، قلنا البكتيريا هي ليست
+
+242
+00:20:07,010 --> 00:20:11,970
+هوائية اختيارية، هي في الأصل هوائية لكن في حال عدم
+
+243
+00:20:11,970 --> 00:20:18,230
+وجود ال oxygen ممكن أنَّها تقدر تنمو وتتكاثر. لكن ال
+
+244
+00:20:18,230 --> 00:20:22,510
+aerotolerant anaerobic bacteria هي البكتيريا هي في
+
+245
+00:20:22,510 --> 00:20:29,650
+الأصل لاهوائية لكن بتتحمل وجود كمية قليلة من ال
+
+246
+00:20:29,650 --> 00:20:34,060
+oxygen (tolerate the presence of oxygen and can
+
+247
+00:20:34,060 --> 00:20:38,220
+survive in air but doesn't require it for growth).
+
+248
+00:20:38,220 --> 00:20:43,620
+هي بتتحمل كمية معينة من ال oxygen لكن ما بتحتاجها
+
+249
+00:20:43,620 --> 00:20:48,600
+في النمو لأنها هي بالأصل بكتيريا لاهوائية. طيب بما
+
+250
+00:20:48,600 --> 00:20:51,820
+أنَّها بكتيريا لاهوائية، عشان تحصل على الطاقة
+
+251
+00:20:51,820 --> 00:20:55,340
+بتستخدم anaerobic respiration وال
+
+252
+00:20:55,340 --> 00:21:00,400
+fermentation فقط لأنها هي بالأصل لاهوائية، مش هتقدر
+
+253
+00:21:00,680 --> 00:21:03,980
+بتستخدم ال aerobic respiration للحصول على الطاقة
+
+254
+00:21:03,980 --> 00:21:07,160
+فقط. الآن، aerobic respiration وال fermentation هي
+
+255
+00:21:07,160 --> 00:21:12,520
+اللي هتحصل من خلالهم على الطاقة. طيب، (they cause
+
+256
+00:21:12,520 --> 00:21:17,560
+superoxide dismutase). ليش بتقدر هذه البكتيريا تتحمل
+
+257
+00:21:17,560 --> 00:21:22,440
+كمية معينة من الأكسجين؟ نتيجة وجود إنزيم ال
+
+258
+00:21:22,440 --> 00:21:27,700
+superoxide dismutase، وبالتالي ممكن أنَّها تقدر تتحمل
+
+259
+00:21:27,700 --> 00:21:31,700
+كمية معينة لأنه موجود فقط إنزيم ال super oxide
+
+260
+00:21:31,700 --> 00:21:35,320
+dismutase. وبالتالي هتتخلص من ال toxic form of
+
+261
+00:21:35,320 --> 00:21:39,220
+أكسجين. يبقى تحتوي فقط على هذا الإنزيم، عشان هيك
+
+262
+00:21:39,220 --> 00:21:44,440
+بتقدر تتحمل كمية معينة من الأكسجين. لو زاد عن هذه
+
+263
+00:21:44,440 --> 00:21:51,000
+الكمية من الأكسجين هتموت البكتيريا. طيب لو أنا بدي
+
+264
+00:21:51,000 --> 00:21:57,210
+أنمي هذه البكتيريا في tube، تمام؟ هلاقي أنَّها ممكن
+
+265
+00:21:57,210 --> 00:22:02,030
+تقدر تنمو طبعًا في كل tube لكن أكثر مكان هتنمو
+
+266
+00:22:02,030 --> 00:22:05,990
+فيه اللي هو تحت في الأسفل، ال sediment. هذا بالنسبة
+
+267
+00:22:05,990 --> 00:22:11,870
+لل aerotolerant anaerobic bacteria. آخر تصنيف اللي هو
+
+268
+00:22:11,870 --> 00:22:18,370
+ال microaerophilic، اللي هي بتنمو فقط في
+
+269
+00:22:18,370 --> 00:22:22,430
+حال كان في عندي concentration قليل من الأكسجين.
+
+270
+00:22:27,590 --> 00:22:32,350
+في حال كان عندي كمية قليلة من ال oxygen بتحتاج
+
+271
+00:22:32,350 --> 00:22:37,710
+كمية قليلة من ال oxygen عشان تنمو، لكن مش الكمية
+
+272
+00:22:37,710 --> 00:22:41,550
+اللي بتحتاجها الهوائية الإجبارية. إحنا قلنا أنَّ
+
+273
+00:22:41,550 --> 00:22:44,330
+الهوائية الإجبارية (obligate aerobic bacteria)
+
+274
+00:22:44,330 --> 00:22:48,890
+بتحتاج oxygen حتى تنمو. هذه نفس الفكرة، بتحتاج ال
+
+275
+00:22:48,890 --> 00:22:52,830
+oxygen حتى تنمو لكن بتحتاج كمية قليلة من ال oxygen
+
+276
+00:22:52,830 --> 00:22:57,940
+حتى تنمو (أقل من 5-10% من ال oxygen)
+
+277
+00:22:57,940 --> 00:23:03,360
+فقط حتى تنمو البكتيريا. بتحتاج كمية من 5-10% أو
+
+278
+00:23:03,360 --> 00:23:07,580
+أقل من 5-10% من ال oxygen عشان تقدر
+
+279
+00:23:07,580 --> 00:23:13,520
+تنمو وتتكاثر. طيب عشان تحصل على الطاقة رح تحصل على
+
+280
+00:23:13,520 --> 00:23:18,160
+الطاقة فقط من ال aerobic respiration. رح تنمو على
+
+281
+00:23:18,160 --> 00:23:24,040
+السطح فقط. تمام؟ أو رح تنمو، مش على السطح، أسفل من
+
+282
+00:23:24,040 --> 00:23:28,200
+السطح بقليل، يعني هيكون أقل من السطح، مش على السطح
+
+283
+00:23:28,200 --> 00:23:32,920
+شوية تحت من السطح لأنها هي بتحتاج كمية قليلة من
+
+284
+00:23:32,920 --> 00:23:38,760
+الأكسجين. رح تحتوي هي على كل أنواع الإنزيمات
+
+285
+00:23:38,760 --> 00:23:43,000
+اللازمة للتخلص من ال toxic form. بكتيريا هذا
+
+286
+00:23:43,000 --> 00:23:47,780
+بالنسبة لتصنيفات البكتيريا حسب حاجتها للأكسجين.
+
+287
+00:23:53,740 --> 00:23:59,860
+البكتيريا الـ Capnophilic
+
+288
+00:23:59,860 --> 00:24:06,880
+بيحتاج ال CO2 حتى تنمو وتتكاثر. طب نيجي لهاي ال
+
+289
+00:24:06,880 --> 00:24:10,200
+slide، هاي ال slide لخصت تقريبًا كل اللي ذكرنا
+
+290
+00:24:10,200 --> 00:24:15,300
+تصنيفات البكتيريا حسب حاجتها للأكسجين. نيجي ل ال
+
+291
+00:24:15,300 --> 00:24:18,600
+obligate aerobic، قلنا عشان تحصل على الطاقة من
+
+292
+00:24:18,600 --> 00:24:22,420
+خلال ال cellular respiration، من خلال ال aerobic
+
+293
+00:24:22,420 --> 00:24:26,600
+respiration أو ال cellular respiration فقط للحصول
+
+294
+00:24:26,600 --> 00:24:32,120
+على الطاقة. هي aerobic مش anaerobic. لما أنميها في
+
+295
+00:24:32,120 --> 00:24:36,500
+broth tube، هلاقي أنَّها موجودة على السطح. بالنسبة
+
+296
+00:24:36,500 --> 00:24:40,920
+للإنزيمات، كل الإنزيمات موجودة عندها، هي ال
+
+297
+00:24:40,920 --> 00:24:43,500
+obligate aerobic bacteria. طبعًا
+
+298
+00:24:43,500 --> 00:24:46,800
+ال peroxidase وال catalase، واحد منهم هيكون موجود، مش
+
+299
+00:24:46,800 --> 00:24:50,140
+الاثنين، واحد منهم. البكتيريا اللي عندها catalase مش
+
+300
+00:24:50,140 --> 00:24:54,170
+هيكون عندها peroxidase والعكس صحيح. طب نيجي لأ الـ
+
+301
+00:24:54,170 --> 00:24:57,650
+facultative anaerobic bacteria، قلنا هي لاهوائية
+
+302
+00:24:57,650 --> 00:25:03,990
+اختيارية، هي بالأصل هوائية. يبقى عشان تحصل على
+
+303
+00:25:03,990 --> 00:25:07,690
+الطاقة من خلال ال cellular respiration وكمان
+
+304
+00:25:07,690 --> 00:25:13,370
+بتقدر تنمو في عدم وجود ال oxygen، فبالتالي من
+
+305
+00:25:13,370 --> 00:25:16,450
+خلال ال anaerobic respiration وال fermentation
+
+306
+00:25:16,450 --> 00:25:19,390
+بتحصل على الطاقة. يبقى بشكل عام، ال respiration بـ
+
+307
+00:25:19,390 --> 00:25:22,450
+(cellular respiration, aerobic respiration) وال fermentation
+
+308
+00:25:22,450 --> 00:25:26,790
+هتحصل على الطاقة. هي aerobic وanaerobic بكتيريا.
+
+309
+00:25:26,790 --> 00:25:29,810
+قلنا بما أنَّها هي هوائية يبقى هيكون عندها كل
+
+310
+00:25:29,810 --> 00:25:34,110
+ال إنزيمات. النمو في ال broth tube هيكون في كل ال
+
+311
+00:25:34,110 --> 00:25:37,770
+tubes لكن أكثر شيء هيكون على السطح، لأنها هي قلنا
+
+312
+00:25:37,770 --> 00:25:43,510
+بأصلها هي هوائية، بتحب أو بتفضل وجود الأكسجين.
+
+313
+00:25:43,510 --> 00:25:49,290
+بالنسبة لل obligate anaerobic bacteria، هي بكتيريا
+
+314
+00:25:49,290 --> 00:25:52,470
+لاهوائية (Anaerobic bacteria)، مش Aerobic. يبقى
+
+315
+00:25:52,470 --> 00:25:55,770
+فبالتالي فقط عشان تحصل على الطاقة من خلال ال
+
+316
+00:25:55,770 --> 00:25:59,950
+fermentation أو من خلال ال anaerobic respiration
+
+317
+00:25:59,950 --> 00:26:04,410
+أو زي ما ذكرنا ال autotrophy، اللي هي ال anaerobic
+
+318
+00:26:04,410 --> 00:26:07,310
+respiration. أكيد بما أنَّها anaerobic مش هيكون في
+
+319
+00:26:07,310 --> 00:26:10,690
+عندها ولا إنزيم من الإنزيمات للتخلص من ال toxic
+
+320
+00:26:10,690 --> 00:26:14,710
+form. البكتيريا هي بتموت في وجود ال oxygen نتيجة
+
+321
+00:26:14,710 --> 00:26:18,770
+عدم وجود الإنزيمات للتخلص من ال toxic form
+
+322
+00:26:18,770 --> 00:26:23,890
+للأكسجين. نموها في ال broth tube هيكون في الأسفل، في
+
+323
+00:26:23,890 --> 00:26:28,950
+ال sediment فقط، مش هيكون في على السطح لأنها بتنمو
+
+324
+00:26:28,950 --> 00:26:32,710
+فقط في عدم وجود الأكسجين. ال Aerotolerant Anaerobic
+
+325
+00:26:32,710 --> 00:26:37,090
+قلنا هي في الأصل anaerobic bacteria لاهوائية لكن
+
+326
+00:26:37,090 --> 00:26:44,130
+بتتحمل اللي هو كمية قليلة من الأكسجين فقط، بتتحمل
+
+327
+00:26:44,130 --> 00:26:47,870
+كمية قليلة من الأكسجين، بتقدر نتيجة وجود ال super
+
+328
+00:26:47,870 --> 00:26:49,850
+oxide dismutase enzyme.
+
+329
+00:26:52,460 --> 00:26:56,880
+من خلال، أو من خلال ال fermentation بتحصل على
+
+330
+00:26:56,880 --> 00:27:00,520
+الطاقة، وكمان من خلال اللي هو ال anaerobic
+
+331
+00:27:00,520 --> 00:27:03,420
+respiration. يبقى هنضيف anaerobic respiration.
+
+332
+00:27:03,420 --> 00:27:07,800
+بما أنَّها لاهوائية، فبالتالي بتحصل على الطاقة من
+
+333
+00:27:07,800 --> 00:27:11,660
+خلال التنفس الخلوي أو من خلال التنفس اللاهوائي
+
+334
+00:27:11,660 --> 00:27:14,580
+اللي هو ال anaerobic respiration من خلال ال
+
+335
+00:27:14,580 --> 00:27:21,430
+fermentation. هلاحظ أنَّه بالنسبة لل Aerotolerant
+
+336
+00:27:21,430 --> 00:27:28,790
+anaerobic bacteria، هتكون طبعًا اللي هو نموها
+
+337
+00:27:28,790 --> 00:27:35,130
+هيكون هي طبعًا لاهوائية، هيكون في كل tube، أكثر شيء
+
+338
+00:27:35,130 --> 00:27:39,290
+هيكون في الأسفل لأنها لاهوائية، لكن ممكن أني أنا
+
+339
+00:27:39,290 --> 00:27:43,510
+ألاقيها في الأعلى لأنها بتتحمل كمية معينة من ال
+
+340
+00:27:43,510 --> 00:27:50,550
+oxygen. بالنسبة لآخر شيء، ال microaerophilic bacteria،
+
+341
+00:27:50,550 --> 00:27:56,230
+طبعًا عشان تحصل على الطاقة فقط من خلال ال cellular
+
+342
+00:27:56,230 --> 00:27:59,490
+respiration أو ال aerobic respiration، زي ال
+
+343
+00:27:59,490 --> 00:28:04,650
+obligate aerobic، لأنها تحتاج للنمو، بتحتاج ال oxygen
+
+344
+00:28:04,650 --> 00:28:08,550
+لكن بكمية قليلة، أقل مما تحتاج ال obligate aerobic
+
+345
+00:28:08,550 --> 00:28:14,350
+بكتيريا. عشان هيك بشوفها أقل بقليل من، مش على السطح،
+
+346
+00:28:14,350 --> 00:28:21,170
+يعني أقل من السطح شوية، تحت من السطح. بتنمو هي
+
+347
+00:28:21,170 --> 00:28:24,390
+aerobic فقط، مش anaerobic. موجود فيها كل الإنزيمات
+
+348
+00:28:24,390 --> 00:28:28,730
+للتخلص من ال toxic form. أمثلة على ال obligate
+
+349
+00:28:28,730 --> 00:28:32,030
+aerobic bacteria: البكتريا البصلية والزائفة (pseudomonas). الفصيلة الـ
+
+350
+00:28:32,030 --> 00:28:35,250
+facultative anaerobic bacteria: كل ال enterobacteriaceae بما فيهم
+
+351
+00:28:35,250 --> 00:28:39,780
+ال E.coli والـ staphylococcus. بالنسبة للـ Obligate
+
+352
+00:28:39,780 --> 00:28:43,080
+Anaerobic Bacteria: عندي ال Clostridium، ال
+
+353
+00:28:43,080 --> 00:28:46,500
+Aerotolerant Anaerobic Bacteria: ال Lactobacillus،
+
+354
+00:28:46,500 --> 00:28:49,940
+ال microaerophilic: اللي هي ال Campylobacter وال
+
+355
+00:28:49,940 --> 00:28:53,780
+Helicobacter pylori. هذا بالنسبة للجدول طبعًا،
+
+356
+00:28:53,780 --> 00:28:58,660
+بالنسبة للأمثلة، مطلوب منكم حفظ الأمثلة ومهمة
+
+357
+00:28:58,660 --> 00:29:04,760
+جدًا الأمثلة. هذا تقريبًا الجدول ملخص لكل اللي ذكرناه
+
+358
+00:29:04,760 --> 00:29:10,540
+عن تصنيفات البكتيريا حسب حاجتها للأكسجين. طيب هلأ
+
+359
+00:29:10,540 --> 00:29:17,640
+نيجي للخطوات عشان أعرف شو تصنيفات البكتيريا حسب
+
+360
+00:29:17,640 --> 00:29:22,480
+حاجتها للأكسجين، بقول لي أني أنا بستخدم ميديا brain heart
+
+361
+00:29:22,480 --> 00:29:26,700
+infusion agar في Petri dishes، لكن أنا بستخدم ال
+
+362
+00:29:26,700 --> 00:29:30,960
+brain heart infusion agar. مكوناتها: peptone، mixed
+
+363
+00:29:30,960 --> 00:29:34,220
+starch. البيبتون بيكون هو عبارة عن بروتين، مصدر
+
+364
+00:29:34,220 --> 00:29:38,660
+للكربون والنيتروجين اللازم لنمو البكتيريا. beef heart
+
+365
+00:29:38,660 --> 00:29:42,380
+infusion طبعًا، beef heart اللي هو اللحم البقري، brain heart
+
+366
+00:29:42,380 --> 00:29:47,280
+infusion، glucose، يعني جل sodium chloride طبعًا عشان
+
+367
+00:29:47,280 --> 00:29:51,860
+أحافظ على ال osmotic pressure، dipotassium
+
+368
+00:29:51,860 --> 00:29:55,000
+phosphate اللي هو ال buffer عشان يعادل ال pH،
+
+369
+00:29:55,000 --> 00:30:01,480
+glucose طبعًا اللي هو ال nutrient، اللي هو السكر. في
+
+370
+00:30:01,480 --> 00:30:05,640
+عندي أكيد، بما أنَّه agar أكيد هيكون نسبة agar 1.5%
+
+371
+00:30:05,640 --> 00:30:10,920
+عشان أعمل اللي هو أو أشوف تصنيفات البكتيريا حسب
+
+372
+00:30:10,920 --> 00:30:17,360
+حاجتها للأكسجين، بقول لي هتستخدمي أنبوبتين أو ثلاثة
+
+373
+00:30:17,360 --> 00:30:23,040
+من brain heart infusion agar. دي هستخدم أنابيب
+
+374
+00:30:23,040 --> 00:30:26,860
+الأنابيب هي هيكون مصبوب فيها media brain heart
+
+375
+00:30:26,860 --> 00:30:32,750
+infusion agar، هصبها بـ deep، هتكون deep اللي هو مش
+
+376
+00:30:32,750 --> 00:30:35,890
+هعملها طبعًا بشكل مائل، فقط بعد ما تطلع من ال
+
+377
+00:30:35,890 --> 00:30:41,190
+autoclave، هخليها تتصلب بشكل عادي، بشكل مستقيم. بدي
+
+378
+00:30:41,190 --> 00:30:45,050
+hot plate، water bath، ice water bath، و thermometer.
+
+379
+00:30:45,050 --> 00:30:52,550
+طيب شو الخطوات؟ بقول لي بتجيبي اللي هو ال brain
+
+380
+00:30:52,550 --> 00:30:57,680
+heart infusion agar في أنابيب، هذول طبعًا بعد ما أنتِ
+
+381
+00:30:57,680 --> 00:31:01,860
+جهزتيهم وحضرتي ال media، وطبعًا طلعتيهم من ال
+
+382
+00:31:01,860 --> 00:31:05,660
+autoclave، اتصلبوا. وقلنا بنحط هاي الأنابيب في
+
+383
+00:31:05,660 --> 00:31:11,020
+boiling water bath، بنجهز water bath على درجة حرارة
+
+384
+00:31:11,020 --> 00:31:15,420
+أكثر من 100، طبعًا boiling water bath يعني أن ال
+
+385
+00:31:15,420 --> 00:31:18,200
+water bath بتكون المية بتغلي، يعني درجة الحرارة
+
+386
+00:31:18,200 --> 00:31:23,330
+أكثر من 100. طيب، after the brain heart infusion agar
+
+387
+00:31:23,330 --> 00:31:27,970
+ذابت، أكيد بما أنَّه درجة حرارة المية، أكيد الأجار قلنا
+
+388
+00:31:27,970 --> 00:31:32,670
+بتصلب عند 45 درجة سليزية، فأكيد هيكون هيتصلب اللي
+
+389
+00:31:32,670 --> 00:31:38,360
+هو الأجار في هاي ال media. بقول لي لما أتأكد أنَّه ال
+
+390
+00:31:38,360 --> 00:31:44,800
+agar اللي هو ذاب، أو sorry، هو طبعًا بـ بيدوب عند
+
+391
+00:31:44,800 --> 00:31:49,980
+درجة حرارة 85 درجة سليزية، بيدوب عند 85
+
+392
+00:31:49,980 --> 00:31:56,160
+درجة سليزية وبتصلب عند 45، فبالتالي أكيد عند
+
+393
+00:31:56,160 --> 00:32:01,100
+بما أنَّه درجة حرارة ال water bath مية أكيد هي هي
+
+394
+00:32:01,100 --> 00:32:07,800
+ذابت هذا ال agar. بقول لي لما أتأكد أنَّ الأجار ذاب، بتخلي
+
+395
+00:32:07,800 --> 00:32:12,160
+اللي هم الأنابيب في ال rack أو بتخلي ال water
+
+396
+00:32:12,160 --> 00:32:18,80
+
+445
+00:36:16,870 --> 00:36:19,930
+الـ toxic form بقول لي في الـ anaerobic bacteria ما
+
+446
+00:36:19,930 --> 00:36:26,810
+بقدر أتخلص من الـ toxic form للأكسجين، no detoxify
+
+447
+00:36:26,810 --> 00:36:31,050
+pathway، ما في عندي أي طرق لإزالة سمية الـ toxic
+
+448
+00:36:31,050 --> 00:36:35,190
+form of الأكسجين، فبالتالي البكتيريا هتموت، لكن في الـ
+
+449
+00:36:35,190 --> 00:36:38,450
+anaerobic bacteria و الـ facultative anaerobic
+
+450
+00:36:38,450 --> 00:36:42,350
+bacteria، الـ toxic form of الأكسجين بيتم إزالة
+
+451
+00:36:42,350 --> 00:36:45,980
+السمية عن طريق الإنزيمات، التالي الـ super oxide
+
+452
+00:36:45,980 --> 00:36:51,040
+dismutase، الكاتلاز، والبيروكسيداز، فبالتالي بيتكون non
+
+453
+00:36:51,040 --> 00:36:55,800
+-toxic form اللي هي الـ water و الـ oxygen، البكتيريا
+
+454
+00:36:55,800 --> 00:37:01,520
+بتقدر تعيش، طيب كيف أنا بدي أنمي البكتيريا اللي لا
+
+455
+00:37:01,520 --> 00:37:06,640
+هوائية؟ أول طريقة عندي اللي هي الـ fluid
+
+456
+00:37:06,640 --> 00:37:11,760
+thioglycolate broth، بقول لي الـ fluid thioglycolate
+
+457
+00:37:11,760 --> 00:37:16,230
+broth is a reducing media، reducing media، ليش
+
+458
+00:37:16,230 --> 00:37:20,290
+reducing media؟ بقول لي لأنه بتحتوي على الـ Sodium
+
+459
+00:37:20,290 --> 00:37:25,870
+Thioglycolate، هذا Sodium Thioglycolate أحد مكونات
+
+460
+00:37:25,870 --> 00:37:30,350
+الـ media، الـ fluid Thioglycolate، شو وظيفته
+
+461
+00:37:30,350 --> 00:37:35,380
+removes O2 from the media؟ بيزيل الأكسجين الموجود
+
+462
+00:37:35,380 --> 00:37:38,780
+في الـ media، فبالتالي هيك بقدر أنمي البكتيريا
+
+463
+00:37:38,780 --> 00:37:43,060
+اللاهوائية، يبقى عرفنا ليش هي تعتبر reducing media
+
+464
+00:37:43,060 --> 00:37:46,580
+لأنها بتحتوي على الـ sodium-thioglycolate اللي
+
+465
+00:37:46,580 --> 00:37:50,280
+بيزيل الأكسجين من الـ media، فبالتالي بوفير ظروف
+
+466
+00:37:50,280 --> 00:37:54,500
+للاهوائية، هذه الـ media بتدعم الـ aerobic and
+
+467
+00:37:54,500 --> 00:37:59,410
+anaerobic بكتيريا، بقول لي في هاي الـ media فيه كاشف
+
+468
+00:37:59,410 --> 00:38:03,890
+هذا الكاشف عشان أعرف هل تم التخلص من الأكسجين ولا
+
+469
+00:38:03,890 --> 00:38:12,710
+لا، Resazurin is an oxidation reduction indicator
+
+470
+00:38:12,710 --> 00:38:16,650
+that turned to pink in the presence of O2، هذا الـ
+
+471
+00:38:16,650 --> 00:38:21,270
+indicator بيتحول للون الـ pink في حال وجود الأكسجين،
+
+472
+00:38:21,270 --> 00:38:26,530
+يبقى في حال وجود الأكسجين بيتحول للون الـ pink، هيك
+
+473
+00:38:26,530 --> 00:38:33,030
+بعرف إنه والله إنه الأكسجين موجود في هاي الـ
+
+474
+00:38:33,030 --> 00:38:37,610
+media، لم يتم التخلص منه، بقول لي small amount of agar
+
+475
+00:38:37,610 --> 00:38:41,690
+في عندي كمية قليلة موجودة في الـ media to retard
+
+476
+00:38:41,690 --> 00:38:47,300
+gases، ليش موجود في عندي نسبة أجار أو أجار بنسبة
+
+477
+00:38:47,300 --> 00:38:53,640
+قليلة جداً؟ لأن الأجار بيعمل Trapping، بيحجز البخار
+
+478
+00:38:53,640 --> 00:38:59,420
+وبيحجز الغازات والأكسجين، فبالتالي بيمنع انتشارها
+
+479
+00:38:59,420 --> 00:39:04,640
+في الـ Lithium، وبيوفر هيك ظروف لاهوائية، فيه المعلومة
+
+480
+00:39:05,000 --> 00:39:09,560
+إنه أنا الـ Thioglycolate broth بروح أنا بسخّنها
+
+481
+00:39:09,560 --> 00:39:13,340
+عشان أطرد أي غازات موجودة في الـ medium، إحنا قلنا
+
+482
+00:39:13,340 --> 00:39:18,520
+قبل شوية، في كيف أنا بدي أوفر ظروف أو أشوف كيف
+
+483
+00:39:18,520 --> 00:39:22,160
+تصنيفات البكتيريا حسب حاجتها للأكسجين، قلنا بعد
+
+484
+00:39:22,160 --> 00:39:29,840
+ما أنا آخذ اللي هو أحط التيوبات في الـ water bath
+
+485
+00:39:29,840 --> 00:39:31,900
+لما أتأكد إنه
+
+486
+00:39:37,270 --> 00:39:42,710
+عشان أضمن إن كل الغازات اللي موجودة في الـ media تمّ
+
+487
+00:39:42,710 --> 00:39:47,310
+طردها، هكذا أنا وفرت الظروف اللاهوائية، هذا بالنسبة لـ
+
+488
+00:39:47,740 --> 00:39:52,660
+الأول طريقة لتنمية البكتيريا اللاهوائية، لـ Fluid
+
+489
+00:39:52,660 --> 00:39:56,400
+Thioglycolate Broth، هي Reducing Media، وقلنا ليش
+
+490
+00:39:56,400 --> 00:39:59,720
+Reducing Media؟ شو الـ Indicator الموجود في هذه
+
+491
+00:39:59,720 --> 00:40:05,300
+الـ Media؟ طبعاً موجود، أكيد نسبة أجار قليلة عشان
+
+492
+00:40:05,300 --> 00:40:09,300
+تعمل Trapping للغازات والأكسجين، وأوفر ظروف
+
+493
+00:40:09,300 --> 00:40:14,990
+لاهوائية، تاني طريقة عشان أنا أوفر ظروف لاهوائية، الـ
+
+494
+00:40:14,990 --> 00:40:20,190
+gas bag system، الـ anaerobic jar، anaerobic jar
+
+495
+00:40:20,190 --> 00:40:24,150
+such as the gas bag are visible in which an
+
+496
+00:40:24,150 --> 00:40:28,310
+anaerobic environment، بقول لي هو عشان أوفر ظروف
+
+497
+00:40:28,310 --> 00:40:32,490
+لاهوائية، هذه الظروف اللاهوائية كيف أنا بوفرها من
+
+498
+00:40:32,490 --> 00:40:37,010
+خلال؟ is generated after inoculate media are sealed
+
+499
+00:40:37,010 --> 00:40:43,270
+into the chamber، بقول لي إن الـ anaerobic jar هو
+
+500
+00:40:43,270 --> 00:40:48,410
+عبارة عن مرتبان محكم الإغلاق، sealed يعني محكم
+
+501
+00:40:48,410 --> 00:40:55,360
+الإغلاق، بجيب البكتيريا اللي أنا نمتها أو اللي أنا
+
+502
+00:40:55,360 --> 00:40:59,700
+بالأول زرعتها، طبعاً زرعت بكتيريا على ميديا high
+
+503
+00:40:59,700 --> 00:41:04,000
+البكتيريا بتحتاج ظروف لاهوائية، زرعتها على أطباق
+
+504
+00:41:04,000 --> 00:41:08,880
+بروح بحط الأطباق، الميديا المزروعة في هذا
+
+505
+00:41:08,880 --> 00:41:14,700
+الـ chamber، وبسكره، طبعاً قبل ما أنا أسكره، بقول لي في
+
+506
+00:41:14,700 --> 00:41:21,360
+في هذا الـ jar يكون اللي هو الـ Gas Generator
+
+507
+00:41:21,360 --> 00:41:26,600
+Envelope، إيش هذا الـ Gas Generator Envelope؟ عبارة
+
+508
+00:41:26,600 --> 00:41:32,800
+عن كيس، هذا الكيس موجود فيه مواد كيميائية، تمام، يبقى
+
+509
+00:41:32,800 --> 00:41:36,280
+موجود، عبارة، اللي بالنسبة ل الآن الـ Aerobic Jar
+
+510
+00:41:36,280 --> 00:41:41,180
+عبارة عن مرتبان، بروح أنا بحط الأطباق اللي أنا زرعت
+
+511
+00:41:41,180 --> 00:41:44,980
+البكتيريا اللي بتحتاج في ظروف لاهوائية، بحطها
+
+512
+00:41:44,980 --> 00:41:49,880
+upside down في هذا الـ jar، على جانب بحط كيس اللي هو الـ
+
+513
+00:41:49,880 --> 00:41:54,960
+gas generator، generator envelope، هذا الكيس موجود
+
+514
+00:41:54,960 --> 00:42:00,700
+فيه مواد كيميائية، بروح بضيف عليه water، تمام، بضيف
+
+515
+00:42:00,700 --> 00:42:03,800
+عليه مية، قبل ما أنا، طبعاً أنا أول شيء بفتح الكيس،
+
+516
+00:42:03,800 --> 00:42:09,100
+بضيف عليه مية، بكون حتى الأطباق upside down، وبعدين
+
+517
+00:42:09,100 --> 00:42:13,880
+بروح بسكر اللي هو هذا المرتبان، بيكون لازم يكون
+
+518
+00:42:13,880 --> 00:42:19,900
+محكم الإغلاق، بقول لي الـ chemicals، طبعاً مكملها اللي
+
+519
+00:42:19,900 --> 00:42:23,060
+هو الـ gas generator envelope that are placed in
+
+520
+00:42:23,060 --> 00:42:28,320
+the jar just prior to sealing، طبعاً إحنا بنحطه قبل
+
+521
+00:42:28,320 --> 00:42:33,040
+ما أنا أسكر المرتبان، روحت سكرت المرتبان، بقول لي الـ
+
+522
+00:42:33,040 --> 00:42:35,920
+chemicals in the envelope، قلنا في مواد كيميائية
+
+523
+00:42:35,920 --> 00:42:41,020
+في هذا الكيس، بقول لي بتنتج، أنا ضفت الـ water طبعاً،
+
+524
+00:42:41,020 --> 00:42:47,540
+فتحته، ضفت الـ water عشان يحفظ التفاعل، بقول لي المواد
+
+525
+00:42:47,540 --> 00:42:50,920
+الكيميائية بيصير في عندي تفاعل كيميائي بينتج
+
+526
+00:42:50,920 --> 00:42:55,600
+hydrogen gas and carbon dioxide، بعد ما أنا سكرت الـ
+
+527
+00:42:55,600 --> 00:43:00,220
+chamber، هذا المرتبان، صار تفاعل، نواتج التفاعل الـ
+
+528
+00:43:00,220 --> 00:43:03,740
+hydrogen gas و الـ carbon dioxide، بقول لي الـ
+
+529
+00:43:03,740 --> 00:43:08,620
+hydrogen gas اللي نتج من التفاعل بيرتبط مع الـ free
+
+530
+00:43:08,620 --> 00:43:13,460
+oxygen الموجود في الـ chamber، يبقى الـ hydrogen gas
+
+531
+00:43:13,460 --> 00:43:16,260
+اللي ناتج من التفاعل بيرتبط مع الأكسجين اللي
+
+532
+00:43:16,260 --> 00:43:19,800
+موجود في الـ chamber، هيك أنا أتخلصت من الأكسجين
+
+533
+00:43:19,800 --> 00:43:24,040
+اللي موجود في الـ chamber، هينتج في النهاية hydrogen
+
+534
+00:43:24,040 --> 00:43:30,760
+gas مع الأكسجين، هينتج عندي water، يبقى بلاحظ
+
+535
+00:43:30,760 --> 00:43:34,560
+إنّه، أو عشان أستدل إنّه صار في عندي ظروف لاهوائية
+
+536
+00:43:34,560 --> 00:43:39,300
+من خلال تعرق الجدار، لأنه بتكون عندي water، بقول الـ
+
+537
+00:43:39,300 --> 00:43:43,640
+carbon، الـ carbon dioxide اللي نتج من التفاعل is
+
+538
+00:43:43,640 --> 00:43:46,480
+required for the growth of certain organisms، في
+
+539
+00:43:46,480 --> 00:43:51,600
+بعض أنواع البكتيريا بتحتاج الـ CO2 لنموها، مثل، في عندي
+
+540
+00:43:51,600 --> 00:43:55,000
+blue indicator strip، في عندي أنا strip، مثل، blue
+
+541
+00:43:55,000 --> 00:44:00,160
+indicator strip موجودة في هذا الـ jar، usually placed
+
+542
+00:44:00,160 --> 00:44:03,620
+in the jar، it turned colorless when the oxygen has
+
+543
+00:44:03,620 --> 00:44:08,470
+been removed، في هذه الطريقة في عندي الـ methylene
+
+544
+00:44:08,470 --> 00:44:13,170
+blue indicator strip، بقول لي بيصير لونها colorless
+
+545
+00:44:13,170 --> 00:44:17,930
+لو الأكسجين تم إزالته، عشان أتأكد إن الأكسجين تمّ
+
+546
+00:44:17,930 --> 00:44:22,170
+إزالته، وتوفر عندي ظروف لاهوائية، بيصير هذا الـ
+
+547
+00:44:22,170 --> 00:44:26,730
+strip لونه colorless، هذا بالنسبة للـ anaerobic jar،
+
+548
+00:44:26,730 --> 00:44:32,810
+هنشوفه في هاي الـ slide، هاي الـ slide هو موضح الـ
+
+549
+00:44:32,810 --> 00:44:38,470
+anaerobic jar، بقول لي، أو إذا إحنا ملاحظين في هذا
+
+550
+00:44:38,470 --> 00:44:43,550
+الـ، هاي الـ slide، في عندي gas generator envelope، في
+
+551
+00:44:43,550 --> 00:44:47,910
+عندي case، هذا الـ case، قلنا فيه مواد كيميائية، أنا
+
+552
+00:44:47,910 --> 00:44:52,610
+أول شيء بصفّ الأطباق upside down، تمام، اللي أنا
+
+553
+00:44:52,610 --> 00:44:56,370
+زرعت فيها البكتيريا بتحتاج ظروف لاهوائية، موجود هذا
+
+554
+00:44:56,370 --> 00:44:59,290
+الكيس، الموجود فيه المواد الكيميائية، بفتح هذا الكيس،
+
+555
+00:44:59,290 --> 00:45:04,550
+بضيف مية، water is added to chemical in envelope،
+
+556
+00:45:04,550 --> 00:45:11,670
+هينتج عندي الـ H2 و CO2 من هذا الـ gas، أو من هذا الـ
+
+557
+00:45:11,670 --> 00:45:17,750
+envelope، تمام، بقول لي إن الـ hydrogen gas هيرتبط مع
+
+558
+00:45:17,750 --> 00:45:21,290
+الأكسجين الموجود في الـ chamber، هيتكون عندي water،
+
+559
+00:45:21,290 --> 00:45:25,050
+فبالتالي هيك أنا قدرت أتخلص من الأكسجين الموجود
+
+560
+00:45:25,050 --> 00:45:30,400
+في الـ chamber، هيك أنا وفرت ظروف لاهوائية، الـ carbon، الـ
+
+561
+00:45:30,400 --> 00:45:35,180
+carbon dioxide، بقول لي بيلزم لـ growth of certain
+
+562
+00:45:35,180 --> 00:45:39,540
+microorganism، هان في عندي، قلنا، الآن الـ indicator
+
+563
+00:45:39,540 --> 00:45:43,960
+strip، اللي هي الـ methylene blue، في حال توفر عندي
+
+564
+00:45:43,960 --> 00:45:47,420
+ظروف لاهوائية، في حال نقص الأكسجين، or absence of
+
+565
+00:45:47,420 --> 00:45:52,530
+oxygen، بيصير لونها colorless، Oxygen is removed from
+
+566
+00:45:52,530 --> 00:45:55,110
+the chamber، الأكسجين بيتم إزالته من الـ chamber
+
+567
+00:45:55,110 --> 00:45:59,330
+من خلال combining with hydrogen، هيرتبط مع الـ
+
+568
+00:45:59,330 --> 00:46:04,750
+hydrogen اللي ناتج من التفاعل، من اللي هو الـ gas in
+
+569
+00:46:04,750 --> 00:46:08,390
+generator envelope to form water، هيتكون عندي
+
+570
+00:46:08,390 --> 00:46:12,030
+بالنهاية water، قلنا بأستدل على التفاعل من خلال
+
+571
+00:46:12,030 --> 00:46:16,830
+تعرق الجدار، لأنه اتكون عندي water، ولهذا التفاعل
+
+572
+00:46:16,830 --> 00:46:20,210
+this reaction، اللي هو ارتباط الـ H، الهيدروجين جاز
+
+573
+00:46:20,210 --> 00:46:25,230
+مع الـ O2، catalyzed by the palladium pellets، بيتم
+
+574
+00:46:25,230 --> 00:46:28,490
+تحفيزه باستخدام الـ palladium pellets اللي بتكون
+
+575
+00:46:28,490 --> 00:46:33,250
+موجودة في الـ chamber، هذا بالنسبة للـ anaerobic jar،
+
+576
+00:46:33,250 --> 00:46:38,390
+طب في عندي نفس الفكرة، لكن مش jar، بيكون gas bag
+
+577
+00:46:38,390 --> 00:46:44,640
+system، اللي هو Case، مش مرتبان، بدل المرتبان بيكون
+
+578
+00:46:44,640 --> 00:46:49,420
+موجود Case، نفس الفكرة، بيكون في Case داخله مواد
+
+579
+00:46:49,420 --> 00:46:53,320
+كيميائية، بروح أنا بفتح الـ Case، بضيف عليه المية،
+
+580
+00:46:53,320 --> 00:47:00,100
+بيصير تفاعل، بوجود محفز معين بيحفظ التفاعل، بيرتبط
+
+581
+00:47:00,100 --> 00:47:04,140
+بالنهاية بينتج، اللي هو بينتج نفس الفكرة اللي كانت
+
+582
+00:47:04,140 --> 00:47:08,770
+في الـ jar، بينتج H2، هيرتبط مع الأكسجين اللي موجود
+
+583
+00:47:08,770 --> 00:47:13,430
+داخل هذا الـ Case، هيك بيوفر ظروف لاهوائية، طبعاً أنا
+
+584
+00:47:13,430 --> 00:47:17,470
+بحط الأطباق في هذا الـ Case، طبعاً مش، مش زي الـ jar،
+
+585
+00:47:17,470 --> 00:47:21,430
+بحط الأطباق في الـ Case، بقول لك هان آخر خطوة إنك أنتَ
+
+586
+00:47:21,430 --> 00:47:28,500
+لازم، طبعاً تستخدمي شريط، عشان أو مثلًا ملقط، تسكري هذا
+
+587
+00:47:28,500 --> 00:47:32,620
+الكيس عشان يوفر الظروف اللاهوائية، بعدين بتاخدي
+
+588
+00:47:32,620 --> 00:47:36,260
+هذا الكيس وبتحطيه في الـ incubation، هيك أنا بوفر
+
+589
+00:47:36,260 --> 00:47:41,020
+ظروف لاهوائية، نفس فكرة المرتبان لكن هو عبارة عن
+
+590
+00:47:41,020 --> 00:47:45,100
+كيس، بحط الأطباق داخل الكيس وبسكر هذا الكيس
+
+591
+00:47:45,100 --> 00:47:49,360
+باستخدام sealing bar، اللي هو شريط أو ملقط، لكن لازم
+
+592
+00:47:49,360 --> 00:47:54,450
+يكون أتأكد إن هو محكم الإغلاق، هذا بالنسبة للجاز باج
+
+593
+00:47:54,450 --> 00:48:00,950
+system، اللي هو الـ Case، نيجي لـ candle jar، الـ candle
+
+594
+00:48:00,950 --> 00:48:04,750
+jar، الـ candle jar is used to create micro
+
+595
+00:48:04,750 --> 00:48:09,490
+aerophilic condition، هذا الـ candle jar يستخدم
+
+596
+00:48:09,490 --> 00:48:13,710
+لتوفير الظروف اللي هي البكتيريا، أو لتنمية
+
+597
+00:48:13,710 --> 00:48:18,120
+البكتيريا، الـ Micro Aerophilic Bacteria، الـ Micro
+
+598
+00:48:18,120 --> 00:48:22,360
+Aerophilic Bacteria، قلنا بتحتاج كمية قليلة من
+
+599
+00:48:22,360 --> 00:48:27,060
+الأكسجين للنمو، شوف فكرة الـ candle jar، هو عبارة عن
+
+600
+00:48:27,060 --> 00:48:33,370
+مرتبان محكم الإغلاق، تمام، محكم الإغلاق، بحط فيه اللي
+
+601
+00:48:33,370 --> 00:48:38,110
+هي الـ medium، البكتيريا اللي أنا، أو الأطباق
+
+602
+00:48:38,110 --> 00:48:43,230
+البكتيريا اللي أنا زرعتها على الـ media، على الطبق
+
+603
+00:48:43,230 --> 00:48:47,930
+اللي بتحتاج ظروف أو بتحتاج كمية قليلة من الـ
+
+604
+00:48:47,930 --> 00:48:52,850
+oxygen، بحطها upside down، بقول لي آخر شيء بتحطي الـ
+
+605
+00:48:52,850 --> 00:48:57,490
+candle، بتشعلي الـ candle، اللي هي الشمعة، ممكن إحنا
+
+606
+00:48:57,490 --> 00:49:03,470
+نجيب slide، بنثبتها على آخر اللي هو petri dish، و
+
+607
+00:49:03,470 --> 00:49:09,070
+بشغل الشمعة، بولّع الشمعة، تمام، وبروح أنا بسكر هذا
+
+608
+00:49:09,070 --> 00:49:12,990
+الـ candle، أو بسكر الـ jar، الـ candle jar، بسكره
+
+609
+00:49:12,990 --> 00:49:17,990
+بعد ما أنا أوّلّع الشمعة، تمام، الـ candle is lit، بضوء
+
+610
+00:49:17,990 --> 00:49:21,190
+الشمعة، and the jar is sealed، وبسكر اللي هو
+
+611
+00:49:21,190 --> 00:49:25,310
+المرتبان، بقول لي الـ candle will burn and reduce
+
+612
+00:49:25,310 --> 00:49:30,240
+the oxygen concentration، هان إنه بعد ما أنتِ تولّعي
+
+613
+00:49:30,240 --> 00:49:36,420
+الشمعة وتسكري المرتبان، الشمعة هتحرق كل الأكسجين
+
+614
+00:49:36,420
\ No newline at end of file
diff --git a/PL9fwy3NUQKwZWgAb_V1ATlRRsH-6i3cPC/Pl7CGd4BP8U_raw.srt b/PL9fwy3NUQKwZWgAb_V1ATlRRsH-6i3cPC/Pl7CGd4BP8U_raw.srt
new file mode 100644
index 0000000000000000000000000000000000000000..81f4402419bef58feb1c38bae5d04f1b0c1bc705
--- /dev/null
+++ b/PL9fwy3NUQKwZWgAb_V1ATlRRsH-6i3cPC/Pl7CGd4BP8U_raw.srt
@@ -0,0 +1,2620 @@
+1
+00:00:01,790 --> 00:00:05,850
+السلام عليكم ورحمة الله وبركاته نستكمل معاكم
+
+2
+00:00:05,850 --> 00:00:10,670
+محاضرات مساق الميكرو بيولوجي العملي في هذا اللقاء
+
+3
+00:00:10,670 --> 00:00:15,030
+هتكون المحاضرة بعنوان bacteria oxygen requirements
+
+4
+00:00:15,030 --> 00:00:19,790
+and anaerobic bacteria قلنا إن البكتيريا عشان تنمو
+
+5
+00:00:19,790 --> 00:00:25,550
+وتتكثر لازم أني أنا أوفر الظروف المناسبة للنمو من
+
+6
+00:00:25,550 --> 00:00:31,040
+هذه الظروف حاجتها للأكسجينهنتعرف في هذه المحاضرة
+
+7
+00:00:31,040 --> 00:00:35,940
+على تصنيفات البكتيريا حسب حاجتها للأكسجين وكيف أنا
+
+8
+00:00:35,940 --> 00:00:40,780
+بدي أقدر أوفر هذه الظروف عشان أقدر أنمي البكتيريا
+
+9
+00:00:40,780 --> 00:00:44,460
+introduction
+
+10
+00:00:44,460 --> 00:00:48,200
+بكتيريا can exist in a wide variety of
+
+11
+00:00:48,200 --> 00:00:51,560
+environments including environments that lack
+
+12
+00:00:51,560 --> 00:00:56,690
+oxygenبقولي إن البكتيريا ممكن إنها تنمو في بيئات
+
+13
+00:00:56,690 --> 00:01:01,930
+مختلفة من ضمن هذه البيئات البيئة اللي مش موجود
+
+14
+00:01:01,930 --> 00:01:06,050
+فيها أكسجين in fact أكسجين is toxic to some
+
+15
+00:01:06,050 --> 00:01:10,330
+bacteria بقولي في الحقيقة الأكسجين يعتبر toxic
+
+16
+00:01:10,330 --> 00:01:16,750
+some لبعض البكتيريا the toxicity is usually due to
+
+17
+00:01:16,750 --> 00:01:20,890
+accidental formation of superoxide and hydrogen
+
+18
+00:01:20,890 --> 00:01:24,700
+peroxidewhich react with other cell components
+
+19
+00:01:24,700 --> 00:01:29,820
+بقولّي أن الـ Oxygen toxic لبعض أنواع البكتيريا
+
+20
+00:01:29,820 --> 00:01:34,600
+قلنا أن الـ Oxygen هو المستقبل النهائي للإلكترونات
+
+21
+00:01:34,600 --> 00:01:40,400
+في الـ aerobic respiration في التنفس الخلوي طبعا
+
+22
+00:01:40,400 --> 00:01:45,380
+في التنفس الخلوي بيرتبط الإلكترون طبعا فيها أنا
+
+23
+00:01:45,380 --> 00:01:49,560
+عندي في الـ electron transport إدشانهي في ندي
+
+24
+00:01:49,560 --> 00:01:55,840
+مضخات تمام لالإلكترونات هيرتبط إلكترون مع اللي هو
+
+25
+00:01:55,840 --> 00:02:01,880
+ال oxygen مع المستقبل النهائي لالإلكترونات هيتكون
+
+26
+00:02:01,880 --> 00:02:07,900
+super oxide هيتكون super oxide اللي هو O2 minus
+
+27
+00:02:07,900 --> 00:02:14,480
+هذا مكتسب إلكترون يعتبر هذا toxicتمام لأنه بيقول
+
+28
+00:02:14,480 --> 00:02:17,940
+يها that this toxicity is usually من ون جاية high
+
+29
+00:02:17,940 --> 00:02:22,520
+toxicity من formation of super oxide كيف بتكون ال
+
+30
+00:02:22,520 --> 00:02:26,000
+super oxide خلال عملية ال aerobic respiration
+
+31
+00:02:26,000 --> 00:02:32,540
+المستقبل أنهيه إلى الإلكترونات هو ال oxygen تمام
+
+32
+00:02:32,540 --> 00:02:36,000
+ال oxygen هو المستقبل أنهيه إلى الإلكترونات هيرتبط
+
+33
+00:02:36,000 --> 00:02:40,320
+مع إلكترون و هيكون ال super oxide طبعا هذا يعتبر
+
+34
+00:02:40,320 --> 00:02:46,010
+toxicبيتم التخلص منه حبر إنزيم الـ super oxide
+
+35
+00:02:46,010 --> 00:02:51,350
+dismutase اتكلمنا في معمل أو chapter الـ Catalysis
+
+36
+00:02:51,350 --> 00:02:56,170
+Test انه انا بتخلص من ال super oxide عن طريق إنزيم
+
+37
+00:02:56,170 --> 00:03:02,830
+ال super oxide dismutase رح يكسره لH2O2
+
+38
+00:03:02,830 --> 00:03:06,550
+اللي هو ال hydrogen peroxide كيف انا بدي اتخلص من
+
+39
+00:03:06,550 --> 00:03:10,630
+ال hydrogen peroxide اللي يعتبر ايضا toxic و سام
+
+40
+00:03:10,960 --> 00:03:16,860
+بيتم التخلص منه اما عن طريق انزيم الكتلاز او عن
+
+41
+00:03:16,860 --> 00:03:21,480
+طريق انزيم الهيدروجين بيروكسيداز طبعا في معادلات
+
+42
+00:03:21,480 --> 00:03:27,240
+شرحناها سابقا شهو نواتج عملية تكسير الهيدروجين
+
+43
+00:03:27,240 --> 00:03:31,540
+بيروكسايد من الكتلاز او بواسطة انزيم الكتلاز و
+
+44
+00:03:31,540 --> 00:03:35,740
+بواسطة انزيم الهيدروجين بيروكسيداز و هنذكره في ال
+
+45
+00:03:35,740 --> 00:03:41,900
+slidات الجائعة تمام هذا بالنسبة لإنه من وين الـ
+
+46
+00:03:41,900 --> 00:03:45,220
+toxicity جاية من تكوين الـ super oxide والهيدروجين
+
+47
+00:03:45,220 --> 00:03:50,900
+بيروكسال بالنسبة للبكتيريا اللي ما عندها هذه
+
+48
+00:03:50,900 --> 00:03:54,180
+الانزيمات اللي ما عندها انزيم ال super oxide
+
+49
+00:03:54,180 --> 00:03:59,180
+dismutase أو الكتلاز أو الهيدروجين بيروكسداز هيكون
+
+50
+00:03:59,180 --> 00:04:05,260
+ال oxygen بالنسبة لها toxic فبالتالي مش هتلجأ
+
+51
+00:04:05,260 --> 00:04:10,590
+لظروف الهوائية مش هتستخدمالـ Aerobic Respiration
+
+52
+00:04:10,590 --> 00:04:16,450
+للحصول للطاقة هتلجأ للظروف اللاهوائية هتلجأ للان
+
+53
+00:04:16,450 --> 00:04:21,750
+Aerobic Respiration هتستخدم inorganic molecule غير
+
+54
+00:04:21,750 --> 00:04:27,050
+الأكسجين كمستقبل نهائي للإلكترونارoxygen species
+
+55
+00:04:27,050 --> 00:04:31,430
+is harmful if bacteria have no enzyme system to
+
+56
+00:04:31,430 --> 00:04:35,550
+eliminate it قولي ال oxygen يعتبر harmful لو
+
+57
+00:04:35,550 --> 00:04:39,610
+البكتيريا ما عندها الانزيمات عشان تتخلص من ال
+
+58
+00:04:39,610 --> 00:04:42,890
+toxic form of oxygen زي ال super oxide و ال
+
+59
+00:04:42,890 --> 00:04:46,470
+hydrogen peroxide إذا ما كان عندها انزيم ال super
+
+60
+00:04:46,470 --> 00:04:50,550
+oxide ديسميوتاز أو انزيم الكتلاز أو ال hydrogen
+
+61
+00:04:50,550 --> 00:04:54,490
+peroxidase هذا هيعتبر بالنسبة لإلها ال oxygen
+
+62
+00:04:54,490 --> 00:05:00,400
+toxicتاني معلومة ان ال oxygen بيكون harmful لو كان
+
+63
+00:05:00,400 --> 00:05:03,860
+ال oxygen species concentration is very high in
+
+64
+00:05:03,860 --> 00:05:09,480
+media or air لو كانت كمية ال oxygen أكتر من الكمية
+
+65
+00:05:09,480 --> 00:05:13,520
+اللي بتحتاجها البكتيريا فبالتالي هان بيكون هو
+
+66
+00:05:13,520 --> 00:05:20,200
+toxic some من نسبة لا البكتيرياطبعاً الـ Oxygen في
+
+67
+00:05:20,200 --> 00:05:27,980
+الـ Air نسبة الـ Oxygen في الهواء 21% إذا كان نسبة
+
+68
+00:05:27,980 --> 00:05:30,860
+الـ Oxygen أعلى موجودة في الـ media أو في الهواء
+
+69
+00:05:30,860 --> 00:05:36,840
+عامة بتحتاجه البكتيريا يعتبر toxic فبالتالي في هذه
+
+70
+00:05:36,840 --> 00:05:41,740
+الحالتين لو البكتيريا ما عندها انزيمات لاتخلص من
+
+71
+00:05:41,740 --> 00:05:46,470
+الـ Oxygen أو من الـ toxic form of Oxygenأو لو كان
+
+72
+00:05:46,470 --> 00:05:50,730
+نسبة الأكسجين في الميديا أو في الهواء أعلى من حاجة
+
+73
+00:05:50,730 --> 00:05:54,430
+أو أعلى من الكمية اللى بتحتاجها البكتيريا هيكون
+
+74
+00:05:54,430 --> 00:05:59,210
+toxic في هذه الحالات أكيد البكتيريا هتلجأ للظروف
+
+75
+00:05:59,210 --> 00:06:05,850
+اللاهوائية للحصول على الطاقة طيب نيجي لاقى ال
+
+76
+00:06:05,850 --> 00:06:13,310
+toxic form of oxygenأول form لأول شكل من أشكال ال
+
+77
+00:06:13,310 --> 00:06:18,590
+oxygen اللي بتكون toxic single oxygen بقول لي is
+
+78
+00:06:18,590 --> 00:06:22,930
+in high energy state extremely reactive exist in
+
+79
+00:06:22,930 --> 00:06:27,970
+phagocyte بقولي هذا هو يعتبر high energy state
+
+80
+00:06:27,970 --> 00:06:33,590
+بيكون extremely reactive نشط بتستخدمه بيتم
+
+81
+00:06:33,590 --> 00:06:38,830
+استخدامه في phagocytosis في البلعمةطبعا الخلايا
+
+82
+00:06:38,830 --> 00:06:45,330
+اللي هي الـ phagocyte لما اتهاجم الميكروب أو
+
+83
+00:06:45,330 --> 00:06:50,250
+البكتيريا وتعمله ابتلاع phagocytosis بتفرز عليه
+
+84
+00:06:50,250 --> 00:06:56,190
+الـ O2 بتفرز عليه single oxygen فبالتالي بيتهاج
+
+85
+00:06:56,190 --> 00:07:00,850
+بتموت البكتيريا أو الميكروب هذا بالنسبة لأول toxic
+
+86
+00:07:00,850 --> 00:07:05,210
+form of oxygenتاني toxic form الـ super oxide free
+
+87
+00:07:05,210 --> 00:07:09,830
+radical والهيدروجين بيروكسايد اللي بيتم إنتاجهم
+
+88
+00:07:09,830 --> 00:07:14,070
+formed in small amount in cellular respiration
+
+89
+00:07:14,070 --> 00:07:18,170
+اللي بيتم إنتاجهم في الcellular respiration أو في
+
+90
+00:07:18,170 --> 00:07:23,560
+ال aerobic respirationهدول so toxic to cell طبعا
+
+91
+00:07:23,560 --> 00:07:28,560
+هدول يعتبروا سمين بيتم التخلص منهم عن طريق طبعا
+
+92
+00:07:28,560 --> 00:07:32,500
+بالنسبة لل super oxide ال free radical بواسطة
+
+93
+00:07:32,500 --> 00:07:36,980
+أنزيم ال super oxide this imutase هيتكون ال
+
+94
+00:07:36,980 --> 00:07:39,480
+hydrogen peroxide كيف بنتخلص من ال hydrogen
+
+95
+00:07:39,480 --> 00:07:44,140
+peroxide إما بواسطة أنزيم الكتلاز أو بواسطة أنزيم
+
+96
+00:07:44,140 --> 00:07:48,500
+ال hydrogen peroxidaseطبعا الـ aerobic respiration
+
+97
+00:07:48,500 --> 00:07:55,580
+اللي هي عملية التنفس الهوائي الخلوي بما أنها نمت
+
+98
+00:07:55,580 --> 00:08:00,520
+في الظروف الهوائية لازم يكون عندها هاي الانزيمات
+
+99
+00:08:00,520 --> 00:08:04,820
+اللي اتكلمنا عنها عشان تتخلص من ال toxic form of
+
+100
+00:08:04,820 --> 00:08:09,520
+oxygen من هاي ال toxic form peroxide anion اللي هي
+
+101
+00:08:09,520 --> 00:08:15,440
+ال O2 لكن minus 2هذا هو super oxide free radical و
+
+102
+00:08:15,440 --> 00:08:20,660
+اكتسب كمان electron آخر used as antimicrobial
+
+103
+00:08:20,660 --> 00:08:25,060
+agent ايش يعني antimicrobial agent يعني مادة
+
+104
+00:08:25,060 --> 00:08:29,660
+تستخدم في قتل أو تثبيت البكتيري هذا معناه ال
+
+105
+00:08:29,660 --> 00:08:33,880
+antimicrobial agent اللي هنتكلم عنه بالتفصيل في
+
+106
+00:08:33,880 --> 00:08:41,410
+آخر chapter في المساقHydroxyl Hydroxyl Radical
+
+107
+00:08:41,410 --> 00:08:47,070
+اللي هو الـ OH- The most active form Form in the
+
+108
+00:08:47,070 --> 00:08:51,310
+cytoplasm from respiration طبعا هو ال most active
+
+109
+00:08:51,310 --> 00:08:56,530
+form و بيكون موجود في ال cytoplasm هذا بالنسبة لل
+
+110
+00:08:56,530 --> 00:09:03,530
+toxic form of oxygen نيجي لكيف أنا بدي أزيل هاي
+
+111
+00:09:03,530 --> 00:09:09,780
+السمية بقول لي toxic form of oxygenممكن اني او انا
+
+112
+00:09:09,780 --> 00:09:15,840
+بحتاج اني انا ازيل هاي السمية ال toxicity لهاي ال
+
+113
+00:09:15,840 --> 00:09:20,320
+form of oxygen باستخدام انزيمات قولنا ال super
+
+114
+00:09:20,320 --> 00:09:26,700
+oxide dismutes بتخلص من اللي هو ال free radical ال
+
+115
+00:09:26,700 --> 00:09:32,900
+O2 minus super oxide free radical الكتلاز بتخلص من
+
+116
+00:09:32,900 --> 00:09:36,320
+ال hydrogen peroxide ال peroxidase بتخلص من ال
+
+117
+00:09:36,320 --> 00:09:41,580
+hydrogen أيضابيروكسايد إذا لم يكن الميكروب ينتج
+
+118
+00:09:41,580 --> 00:09:45,540
+هذه الانزيمات يجب أن يكون لها حالة ايوروبية بقول
+
+119
+00:09:45,540 --> 00:09:50,320
+لو البكتيريا او الميكروب ماعنده هذه الانزيمات لازم
+
+120
+00:09:50,320 --> 00:09:55,420
+البكتيريا تلجأ للظروف اللاهوائية للحصول على الطاقة
+
+121
+00:09:55,420 --> 00:09:58,440
+الخلايا اللي يعيشون في حياتة محتوى أكسجين تحتوي
+
+122
+00:09:58,440 --> 00:10:05,730
+على انزيم سوبر إكسايد ديسميوتاز وكتاليزconvert
+
+123
+00:10:05,730 --> 00:10:09,130
+super oxide and peroxide to oxygen and water
+
+124
+00:10:09,130 --> 00:10:14,430
+بقوللي ان الخلايا اللي بتقدر تعيش في بيئة فيها
+
+125
+00:10:14,430 --> 00:10:18,690
+اكسجين اكيد هتمتلك انزيم ال super oxide ال
+
+126
+00:10:18,690 --> 00:10:23,050
+decimutase و ال catalase طبعا هتحول ال super oxide
+
+127
+00:10:23,050 --> 00:10:27,410
+و ال peroxide ال peroxide هذول طبعا ال toxic form
+
+128
+00:10:27,410 --> 00:10:32,880
+of اكسجين ل oxygen and waterحسب المعادلات اللى
+
+129
+00:10:32,880 --> 00:10:37,960
+هنشوفها في ال slide اللى بعده طبعا ال oxygen و ال
+
+130
+00:10:37,960 --> 00:10:42,700
+water هدول non-toxic form فبالتالي هيك زالت سمية
+
+131
+00:10:42,700 --> 00:10:48,740
+ال ال super oxide ال super oxide و ال peroxide او
+
+132
+00:10:48,740 --> 00:10:53,680
+ال hydrogen peroxide هاي بالنسبة للمعادلات ال
+
+133
+00:10:53,680 --> 00:10:57,920
+super oxide dismutase هذا الانزين بيحول ال super
+
+134
+00:10:57,920 --> 00:11:03,790
+oxide ions طبعاأحنا قلنا الـ super oxide اللي هو
+
+135
+00:11:03,790 --> 00:11:07,470
+الـ free radical هذا يعتبر toxic فعل فبالتالي كيف
+
+136
+00:11:07,470 --> 00:11:10,250
+أنا بدي أتخلص منه في عندي أنا في ال electron a
+
+137
+00:11:10,250 --> 00:11:14,850
+transported chain في عندي hydrogen ion هيتم
+
+138
+00:11:14,850 --> 00:11:18,430
+الارتباط مع ال hydrogen ionباستخدام إنزيم الـ
+
+139
+00:11:18,430 --> 00:11:23,490
+superoxide desimutase هيتكون عندى H2O2 زائد O2
+
+140
+00:11:23,490 --> 00:11:30,410
+قولنا بتكون بالنهاية water و oxygen هذه الـ H2O2
+
+141
+00:11:30,410 --> 00:11:34,630
+تعتبر toxic form كيف أتخلص منها إما باستخدام إنزيم
+
+142
+00:11:34,630 --> 00:11:39,250
+الكتلازأو باستخدام انزيم البروكسيداز بتكسر
+
+143
+00:11:39,250 --> 00:11:43,470
+الكتالياز بتكسر ال hydrogen peroxide اللى تم انتجه
+
+144
+00:11:43,470 --> 00:11:47,050
+أو تم تكوينه من ال free radical اللى هي ال super
+
+145
+00:11:47,050 --> 00:11:51,690
+oxide مع ال hydrogen ion هيتم تكسيره ال hydrogen
+
+146
+00:11:51,690 --> 00:11:56,830
+peroxide بواسطة انزيم الكتالياز ل water and oxygen
+
+147
+00:11:56,830 --> 00:12:01,010
+هيك انا اقدرت اتخلص من ال toxic form of oxygen
+
+148
+00:12:02,200 --> 00:12:06,520
+بالنسبة لـ Pyroxidase نفس الكتالياز بيشتغل على الـ
+
+149
+00:12:06,520 --> 00:12:10,140
+hydrogen pyroxide لكن الـ Pyroxidase أو الـ
+
+150
+00:12:10,140 --> 00:12:14,400
+hydrogen pyroxidase بيحول الـ hydrogen أو بيكسر
+
+151
+00:12:14,400 --> 00:12:20,380
+الـ hydrogen الـ pyroxide ل 2 مليكيول من الميه
+
+152
+00:12:20,380 --> 00:12:26,400
+2H2O يبقى بالنهاية اتكون عندي water و oxygen non
+
+153
+00:12:26,400 --> 00:12:31,060
+-toxic farm يقدر ان انا اتخلص من سمية ال oxygen
+
+154
+00:12:39,020 --> 00:12:44,060
+نيجي لل Oxygen requirement classification تصنيف
+
+155
+00:12:44,060 --> 00:12:48,780
+البكتيريا حسب حاجتها للأكسجين وقلي تم تصنيف
+
+156
+00:12:48,780 --> 00:12:54,460
+البكتيريا حسب حاجتها للأكسجين لخمس تصنيفات أول
+
+157
+00:12:54,460 --> 00:12:58,520
+تصنيف ال obligate Europe طبعا هاي ال obligate
+
+158
+00:12:58,520 --> 00:13:04,760
+Europe معناها هوائية إجبارية require oxygen to
+
+159
+00:13:04,760 --> 00:13:11,930
+liveبتحتاج أو بتستخدم ال oxygen حتى انها تقدر تعيش
+
+160
+00:13:11,930 --> 00:13:19,890
+يبقى هي بتحتاج لل oxygen حتى تقدر تعيش بتستخدمه
+
+161
+00:13:19,890 --> 00:13:26,690
+كمستقبل نهائي للإلكترونات للحصول على الطاقة their
+
+162
+00:13:26,690 --> 00:13:30,480
+metabolic pathway require oxygenas a final
+
+163
+00:13:30,480 --> 00:13:33,600
+electron acceptor خلال الـ aerobic respiration
+
+164
+00:13:33,600 --> 00:13:39,720
+يبقى هي بتستخدم ال aerobic respiration للحصول على
+
+165
+00:13:39,720 --> 00:13:44,280
+الطاقة لأن ال oxygen هو المستقبل النهائي
+
+166
+00:13:44,280 --> 00:13:48,900
+للإلكترونات في عندي طريقة أخرى للحصول على الطاقة
+
+167
+00:13:48,900 --> 00:13:53,320
+بقولي they have no alternate means of producing
+
+168
+00:13:53,320 --> 00:13:56,900
+ATP بقولي ماعندي أي طريقة أخرى للحصول على الطاقة
+
+169
+00:13:56,900 --> 00:14:01,630
+غير ال aerobic respirationلأن هي بتحتاج ال oxygen
+
+170
+00:14:01,630 --> 00:14:05,310
+حتى انها تقدر اتعيش فبالتالي هتستخدم ال oxygen
+
+171
+00:14:05,310 --> 00:14:13,250
+كمستقبل نهائي للإلكترونات طيب بما انها هوائية بما
+
+172
+00:14:13,250 --> 00:14:18,490
+انها هوائية تستخدم ال oxygen كمستقبل نهائي
+
+173
+00:14:18,490 --> 00:14:23,170
+للإلكترونات أكيد راح يكون عندها كل الانزيمات اللي
+
+174
+00:14:23,170 --> 00:14:28,050
+ذكرناها عشان تتخلص من ال toxic form of oxygen عشان
+
+175
+00:14:28,050 --> 00:14:31,820
+تتخلص منالـ Super Oxide الـ Free Radical من الـ
+
+176
+00:14:31,820 --> 00:14:35,340
+Hydrogen Peroxide اللي يتم تكوينهم خلال الـ
+
+177
+00:14:35,340 --> 00:14:39,680
+Aerobic Respiration طيب لو أنا زرعت بكتيريا
+
+178
+00:14:39,680 --> 00:14:44,820
+Obligate Aerobic بكتيريا في برث التيوب راح تنمو
+
+179
+00:14:44,820 --> 00:14:49,660
+البكتيريا بشكل كبير على السطح لأنه في نسبة
+
+180
+00:14:49,660 --> 00:14:54,020
+الأكسوجين على السطح بتكون أعلى ما يكون فهلاحظ إذا
+
+181
+00:14:54,020 --> 00:14:58,970
+كانت البكتيريا Obligate Aerobicهلاحظ أنه النمو
+
+182
+00:14:58,970 --> 00:15:03,490
+البكتيريا هيكون على السطح لأن أعلى نسبة Oxygen
+
+183
+00:15:03,490 --> 00:15:08,430
+بتكون في السطح هذا بالنسبة لأول تصنيف اللي هو
+
+184
+00:15:08,430 --> 00:15:12,310
+Obligate Aerobic Bacteria قولنا بتستخدم Aerobic
+
+185
+00:15:12,310 --> 00:15:16,950
+فقط Respiration للحصول على الطاقة تاني إشي عندها
+
+186
+00:15:16,950 --> 00:15:20,170
+كل الإنزيمات اللي تتخلص من ال toxic form of Oxygen
+
+187
+00:15:20,170 --> 00:15:24,960
+بتنمو علىالسطح طبعا لو أنا نميتها في broth ال tube
+
+188
+00:15:24,960 --> 00:15:30,220
+بتحتاج ال oxygen عشان تقدر اتقش بالنسبة للتصنيف
+
+189
+00:15:30,220 --> 00:15:34,500
+التاني ال facultative anaerobic bacteria اللي هي
+
+190
+00:15:34,500 --> 00:15:40,140
+اللا هوائية اختيارية بقول لي it grows in best
+
+191
+00:15:40,140 --> 00:15:45,700
+where most oxygen is presentedبقول لي بتنمو بوجود
+
+192
+00:15:45,700 --> 00:15:50,440
+ال oxygen او عدمه لكن تفضل وجود ال oxygen يبقى
+
+193
+00:15:50,440 --> 00:15:56,640
+بتقدر تنمو بوجود ال oxygen او في حال عدم وجوده لكن
+
+194
+00:15:56,640 --> 00:16:03,140
+هي بتفضل وجود ال oxygen او بتفضل انها تنمو في وجود
+
+195
+00:16:03,140 --> 00:16:08,420
+ال oxygen يبقى عشان احصل على الطاقة بما انها هي
+
+196
+00:16:08,420 --> 00:16:13,200
+هوائية فبحصل على الطاقة من خلالالـ aerobic
+
+197
+00:16:13,200 --> 00:16:24,440
+respiration يبقى تستهلك الأكسجين للحصول
+
+198
+00:16:24,440 --> 00:16:28,900
+على الطاقة لأنها هوائية تمام بتستخدم الأكسجين
+
+199
+00:16:28,900 --> 00:16:34,560
+للحصول على أو كمستقبل نهائي للإلكترونات طيب إذا ما
+
+200
+00:16:34,560 --> 00:16:40,870
+توفر الأكسجين قلنا بتقدر إنها تعيش طبعافي عدم وجود
+
+201
+00:16:40,870 --> 00:16:44,970
+الأكسجين فبالتالي هتستخدم الـ anaerobic
+
+202
+00:16:44,970 --> 00:16:50,470
+respiration و ال fermentation للحصول على الطاقة في
+
+203
+00:16:50,470 --> 00:16:58,610
+حال عدم وجود الأكسجين تمام طيب بالنسبة للإنزيمات
+
+204
+00:16:58,610 --> 00:17:03,850
+بما أنها هي هوائية في الأصل فبالتالي عندها يكون كل
+
+205
+00:17:03,850 --> 00:17:09,030
+الإنزيمات للتخلص من ال toxic form of oxygen طيب
+
+206
+00:17:09,290 --> 00:17:14,570
+بالنسبة لو أنا نميتها على او في إبرث تيوب هلاحظ
+
+207
+00:17:14,570 --> 00:17:19,430
+انه هذه البكتيريا هتنمو في كل تيوب سواء على السطح
+
+208
+00:17:19,430 --> 00:17:23,950
+او في أسفل التيوب لأنه احنا قلنا هي لها وقية
+
+209
+00:17:23,950 --> 00:17:28,530
+اختيارية بتقدر تنمو في عدم وجود الأكسجين في حال
+
+210
+00:17:28,530 --> 00:17:33,210
+كان مش موجود الأكسجين بتقدر تنمو في حال عدم وجوده
+
+211
+00:17:33,210 --> 00:17:37,450
+يبقى هلاحظ انه في كل تيوب في انديو نمو لأ
+
+212
+00:17:37,450 --> 00:17:42,050
+البكتيريالكن النسبة الأعلى هتكون على السطح لأنها
+
+213
+00:17:42,050 --> 00:17:47,270
+هي قلنا بتفضل وجود ال oxygen هذا بالنسبة لتان
+
+214
+00:17:47,270 --> 00:17:51,710
+تصنيف ال facultative anaerobic bacteria نيجي
+
+215
+00:17:51,710 --> 00:17:56,070
+لتصنيف التالت اللي هو ال obligate anaerobic
+
+216
+00:17:56,070 --> 00:18:01,850
+bacteria اللاهوائية الإجبارية ال growth occurs
+
+217
+00:18:01,850 --> 00:18:06,390
+only when there is no oxygenبقول لي تنمو في غياب
+
+218
+00:18:06,390 --> 00:18:11,650
+الأكسجين فقط انه مو يحدث فقط عند عدم موجود
+
+219
+00:18:11,650 --> 00:18:17,170
+الأكسجين تمام طيب بقول لي انه use anaerobic
+
+220
+00:18:17,170 --> 00:18:21,030
+respiration او fermentation to generate ATP اكيد
+
+221
+00:18:21,030 --> 00:18:26,750
+بما انها لهوائية هتحصل على الطاقة من خلال ال
+
+222
+00:18:26,750 --> 00:18:30,570
+anaerobic respiration ومن خلال ال fermentation مش
+
+223
+00:18:30,570 --> 00:18:34,540
+هيتم استخدام ال anaerobic respirationفي الـ
+
+224
+00:18:34,540 --> 00:18:38,220
+obligate anaerobic لحصول على الطاقة فقط ال
+
+225
+00:18:38,220 --> 00:18:43,580
+anaerobic respiration و ال fermentation طيب ليش ما
+
+226
+00:18:43,580 --> 00:18:50,620
+بتقدر تنمو في وجود ال oxygen بقوللي هي بتفقد
+
+227
+00:18:50,620 --> 00:18:55,880
+الانزيمات اللي هذه الانزيمات من خلالها بنتخلص من
+
+228
+00:18:55,880 --> 00:19:00,560
+ال toxic form لال oxygen لإن سوبر اكساد ديسميوتاز
+
+229
+00:19:00,560 --> 00:19:05,180
+كتلاز بيركسدازفبالتالي هتموت في وجود ال Oxygen
+
+230
+00:19:05,180 --> 00:19:09,400
+يبقى ما عندها الانزيمات للتخلص من ال toxic form of
+
+231
+00:19:09,400 --> 00:19:15,920
+Oxygen فبالتالي مش هتقدر تنمو إلا في حال عدم موجود
+
+232
+00:19:15,920 --> 00:19:20,880
+ال Oxygen لو كان موجود ال Oxygen هتموت die in the
+
+233
+00:19:20,880 --> 00:19:25,100
+presence of Oxygen دي عدم موجود الانزيمات اللازمة
+
+234
+00:19:25,100 --> 00:19:29,540
+للتخلص من ال toxic form of Oxygen يبقى ما عندها
+
+235
+00:19:29,540 --> 00:19:36,110
+الانزيماتاللي ذكرناها لأنها لاهوائية طيب لو أنا
+
+236
+00:19:36,110 --> 00:19:39,890
+زراعت Obligate بكتيريا Obligate anaerobic في broth
+
+237
+00:19:39,890 --> 00:19:44,770
+tube فقط هلاقى النمو في الأسفل هيكون sediment لأن
+
+238
+00:19:44,770 --> 00:19:51,370
+هي تنمو في حال عدم وجود ال oxygen طب نيجي لرابع
+
+239
+00:19:51,370 --> 00:19:56,490
+تصنيف الـ Aerotolerant Anaerobic Bacteria هذه
+
+240
+00:19:56,490 --> 00:20:02,550
+البكتيريا هي بالاصل لاهوائيةلما احنا قلنا على ال
+
+241
+00:20:02,550 --> 00:20:07,010
+faculty of anaerobic قلنا البكتيريا هي هي لا
+
+242
+00:20:07,010 --> 00:20:11,970
+هوائية اختيارية هي في الاصل هوائية لكن في حال عدم
+
+243
+00:20:11,970 --> 00:20:18,230
+وجود ال oxygen ممكن انها تقدر تنمو وتتكثر لكن ال
+
+244
+00:20:18,230 --> 00:20:22,510
+aerotolerant anaerobic بكتيريا هي البكتيريا هي في
+
+245
+00:20:22,510 --> 00:20:29,650
+الاصل لا هوائية لكن بتتحمل وجود كمية قليلة من ال
+
+246
+00:20:29,650 --> 00:20:34,060
+oxygentolerate the presence of oxygen and can
+
+247
+00:20:34,060 --> 00:20:38,220
+survive in air but doesn't require it for growth
+
+248
+00:20:38,220 --> 00:20:43,620
+هي بتتحمل كمية معينة من ال oxygen لكن ما بتحتاجها
+
+249
+00:20:43,620 --> 00:20:48,600
+في النمو لأنه هي بالاصل بكتيريا لا هوائية طيب بما
+
+250
+00:20:48,600 --> 00:20:51,820
+أن هي بكتيريا لا هوائية عشان تحصل على الطاقة
+
+251
+00:20:51,820 --> 00:20:55,340
+بتستخدم الأن Europe crisp by ration وال
+
+252
+00:20:55,340 --> 00:21:00,400
+fermentation فقط لأن هي بالاصل لا هوائية مش هتقدر
+
+253
+00:21:00,680 --> 00:21:03,980
+بتستخدم الـ aerobic respiration للحصول على الطاقة
+
+254
+00:21:03,980 --> 00:21:07,160
+فقط الآن aerobic respiration وال fermentation هي
+
+255
+00:21:07,160 --> 00:21:12,520
+اللي هتحصل من خلالهم على الطاقة طيب، they cause
+
+256
+00:21:12,520 --> 00:21:17,560
+superoxide dismutase ليش بتقدر هذه البكتيريا تتحمل
+
+257
+00:21:17,560 --> 00:21:22,440
+كمية معينة من الأكسجين نتيجة وجود إنزيم ال
+
+258
+00:21:22,440 --> 00:21:27,700
+superoxide dismutaseفبالتالي ممكن انها تقدر تتحمل
+
+259
+00:21:27,700 --> 00:21:31,700
+كمية معينة لانه موجود فقط انزيم ال super oxide
+
+260
+00:21:31,700 --> 00:21:35,320
+dosimutase فبالتالي هتتخلص من ال toxic form of
+
+261
+00:21:35,320 --> 00:21:39,220
+أكسجين يبقى تحتوي فقط على هذا الانزيم عشان هيك
+
+262
+00:21:39,220 --> 00:21:44,440
+بتقدر تتحمل كمية معينة من الأكسجين لو زاد عن هذه
+
+263
+00:21:44,440 --> 00:21:51,000
+الكمية الأكسجين هتموت البكتيريا طيب لو انا بدي
+
+264
+00:21:51,000 --> 00:21:57,210
+أنمي هذه البكتيريا في ال tubeتمام هلاقي انه ممكن
+
+265
+00:21:57,210 --> 00:22:02,030
+تقدر تنمو على طبعا في كل tube لكن اكتر مكان هتنمو
+
+266
+00:22:02,030 --> 00:22:05,990
+فيه اللي هو تحت في الأسفل ال sediment هذا بالنسبة
+
+267
+00:22:05,990 --> 00:22:11,870
+لل aerotolerant aerobic bacteria اخر تصنيف اللي هو
+
+268
+00:22:11,870 --> 00:22:18,370
+ال micro aerobifillas اللي هي growth فقط بتنمو في
+
+269
+00:22:18,370 --> 00:22:22,430
+حال كان في عندي concentration قليل من الأكسجين
+
+270
+00:22:27,590 --> 00:22:32,350
+في حال كان عندى كمية قليلة من ال oxygen بتحتاج
+
+271
+00:22:32,350 --> 00:22:37,710
+كمية قليلة من ال oxygen عشان تنمو لكن مش الكمية
+
+272
+00:22:37,710 --> 00:22:41,550
+اللى بتحتاجها الهوائية الإجبارية احنا قولنا ان
+
+273
+00:22:41,550 --> 00:22:44,330
+الهوائية الإجبارية ال obligate aerobic bacteria
+
+274
+00:22:44,330 --> 00:22:48,890
+بتحتاج oxygen حتى تنمو هذه نفس الفكرة بتحتاج ال
+
+275
+00:22:48,890 --> 00:22:52,830
+oxygen حتى تنمو لكن بتحتاج كمية قليلة من ال oxygen
+
+276
+00:22:52,830 --> 00:22:57,940
+حتى تنموأقل من خمسة لعشرة في المية من ال oxygen
+
+277
+00:22:57,940 --> 00:23:03,360
+فقط حتى تنمو البكتيريا تحتاج كمية من خمسة لعشرة او
+
+278
+00:23:03,360 --> 00:23:07,580
+اقل من خمسة لعشرة في المية من ال oxygen عشان تقدر
+
+279
+00:23:07,580 --> 00:23:13,520
+تنمو و تتكثر طيب عشان احصل على الطاقة رح تحصل على
+
+280
+00:23:13,520 --> 00:23:18,160
+الطاقة فقط من ال aerobic respiration رح تنمو على
+
+281
+00:23:18,160 --> 00:23:24,040
+السطح فقط تمام او رح تنمو مش على السطحأسفل من
+
+282
+00:23:24,040 --> 00:23:28,200
+السطح بقليل يعني هيكون أقل من السطح مش على السطح
+
+283
+00:23:28,200 --> 00:23:32,920
+شوية تحت من السطح لأنه هي بتحتاج كمية قليلة من
+
+284
+00:23:32,920 --> 00:23:38,760
+الأكسجين راح تحتوي هي على كل أنواع الإنزيمات
+
+285
+00:23:38,760 --> 00:23:43,000
+اللازم من التخلص من ال toxic form بكتيريا هذا
+
+286
+00:23:43,000 --> 00:23:47,780
+بالنسبة لتصنيفات البكتيريا حسب حاجتها للأكسجين
+
+287
+00:23:53,740 --> 00:23:59,860
+البكتيريا الـ Cabinophilic
+
+288
+00:23:59,860 --> 00:24:06,880
+بيحتاج الـ CO2 حتى تنمو وتتكثرطب نيجي لهاي ال
+
+289
+00:24:06,880 --> 00:24:10,200
+slide هاي ال slide املخصت قريبا كل اللي ذكرنا
+
+290
+00:24:10,200 --> 00:24:15,300
+تصنيفات البكتيريا حسب حاجتها للأكسجين نيجي ل ال
+
+291
+00:24:15,300 --> 00:24:18,600
+obligate aerobic قولنا عشان تحصل على الطاقة من
+
+292
+00:24:18,600 --> 00:24:22,420
+خلال ال cellular respiration من خلال ال aerobic
+
+293
+00:24:22,420 --> 00:24:26,600
+respiration أو ال cellular respiration فقط للحصول
+
+294
+00:24:26,600 --> 00:24:32,120
+على الطاقةهي aerobic مش anaerobic لما اناميها في
+
+295
+00:24:32,120 --> 00:24:36,500
+broth tube هلاقي انه موفقت على السطح بالنسبة
+
+296
+00:24:36,500 --> 00:24:40,920
+للانزيمات كل الانزيمات موجودة عندها هي ال
+
+297
+00:24:40,920 --> 00:24:43,500
+bacterial obligate aerobic بكتيريا طبعا
+
+298
+00:24:43,500 --> 00:24:46,800
+البروكسيداز والكتاليز واحد منهم هيكون موجود مش
+
+299
+00:24:46,800 --> 00:24:50,140
+الاتنين واحد منهم البكتيريا اللي عندها الكتاليز مش
+
+300
+00:24:50,140 --> 00:24:54,170
+هيكون عندها البروكسيداز والعكس صحيحطب نيجي لأ الـ
+
+301
+00:24:54,170 --> 00:24:57,650
+facultative anaerobic bacteria قولنا هي لا هوائية
+
+302
+00:24:57,650 --> 00:25:03,990
+اختيارية هي بالاصل هوائية يبقى عشان تحصل على
+
+303
+00:25:03,990 --> 00:25:07,690
+الطاقة من خلال الـ Cellular Respiration وكمان
+
+304
+00:25:07,690 --> 00:25:13,370
+بتقدر تنمو في عدم موجود الـ Oxygen فبالتالي من
+
+305
+00:25:13,370 --> 00:25:16,450
+خلال الـ Anaerobic Respiration والـ Fermentation
+
+306
+00:25:16,450 --> 00:25:19,390
+بحصل على الطاقة يبقى بشكل عام الـ Respiration بـ
+
+307
+00:25:19,390 --> 00:25:22,450
+Cellular Run-aerobic respiration والـ fermentation
+
+308
+00:25:22,450 --> 00:25:26,790
+هتحصل على الطاقة هي aerobic وun-aerobic بكتيريا
+
+309
+00:25:26,790 --> 00:25:29,810
+قلنا بما أنها هي هوائية يبقى هيكون عندها كل
+
+310
+00:25:29,810 --> 00:25:34,110
+الانزيمات النمو في الـ breath tube هتكون في كل ال
+
+311
+00:25:34,110 --> 00:25:37,770
+tube لكن أكتر شئ هيكون على السطح لأنه هي قلنا
+
+312
+00:25:37,770 --> 00:25:43,510
+بالاصل هي هوائية بتحب أو تفضل وجود الأكسجين
+
+313
+00:25:43,510 --> 00:25:49,290
+بالنسبة لل obligate un-aerobic bacteriaهي بكتيريا
+
+314
+00:25:49,290 --> 00:25:52,470
+لا هوائية Anaerobic بكتيريا مش Aerobic يبقى
+
+315
+00:25:52,470 --> 00:25:55,770
+فبالتالي فقط عشان تحصل على الطاقة من خلال ال
+
+316
+00:25:55,770 --> 00:25:59,950
+fermentation أو من خلال ال anaerobic respiration
+
+317
+00:25:59,950 --> 00:26:04,410
+أو هان ذكرها ال autotrophy اللي هي ال anaerobic
+
+318
+00:26:04,410 --> 00:26:07,310
+respiration أكيد بما أنها anaerobic مش هيكون في
+
+319
+00:26:07,310 --> 00:26:10,690
+عندها ولا أنزيم من الانزيمات للتخلص من ال toxic
+
+320
+00:26:10,690 --> 00:26:14,710
+foreign بكتيريا هي بتمود في وجود ال oxygen نتيجة
+
+321
+00:26:14,710 --> 00:26:18,770
+عدمووجود الانزيمات للتخلص من الـ toxic form
+
+322
+00:26:18,770 --> 00:26:23,890
+للأكسجين نموها في البروستيتيوب هيكون في الأسفل في
+
+323
+00:26:23,890 --> 00:26:28,950
+ال sediment فقط مش هيكون في على السطح لأن هي بتنمو
+
+324
+00:26:28,950 --> 00:26:32,710
+فقط في وجود الأكسجينالـ Aerotolerant Anaerobic
+
+325
+00:26:32,710 --> 00:26:37,090
+قلنا هي في الأصل Anaerobic بكتيريا لهوائية لكن
+
+326
+00:26:37,090 --> 00:26:44,130
+بتتحمل اللي هو كمية قليلة من الأكسجين فقط بتتحمل
+
+327
+00:26:44,130 --> 00:26:47,870
+كمية قليلة من الأكسجين بتقدر نتيجة وشود ال super
+
+328
+00:26:47,870 --> 00:26:49,850
+oxide desimiotase enzyme
+
+329
+00:26:52,460 --> 00:26:56,880
+من خلال أو من خلال ال fermentation بتحصل على
+
+330
+00:26:56,880 --> 00:27:00,520
+الطاقة وكمان من خلال اللي هو ال unaerobic
+
+331
+00:27:00,520 --> 00:27:03,420
+respiration يبقى هان نضيف ال unaerobic respiration
+
+332
+00:27:03,420 --> 00:27:07,800
+بما أنها لا هوائية فبالتالي بتحصل على الطاقة من
+
+333
+00:27:07,800 --> 00:27:11,660
+خلال التنفس الخلوي أو من خلال التنفس اللاهوائي
+
+334
+00:27:11,660 --> 00:27:14,580
+اللي هو ال unaerobic respiration من خلال ال
+
+335
+00:27:14,580 --> 00:27:21,430
+fermentationهلاحظ أنه بالنسبة للـ Aerotolerant and
+
+336
+00:27:21,430 --> 00:27:28,790
+aerobic bacteria هتكون طبعا اللي هو فيه نموها
+
+337
+00:27:28,790 --> 00:27:35,130
+هيكون هي طبعا لا هوائية هيكون في كل tube أكتر إش
+
+338
+00:27:35,130 --> 00:27:39,290
+هيكون في الأسفل لأن هي لا هوائية لكن ممكن أني أنا
+
+339
+00:27:39,290 --> 00:27:43,510
+ألاقيها في الأعلى لأنه بتتحمل كمية معينة من ال
+
+340
+00:27:43,510 --> 00:27:50,550
+oxygenبالنسبة لأخر شيء ال micro aerophilic بكتيريا
+
+341
+00:27:50,550 --> 00:27:56,230
+طبعا عشان تحصل على الطاقة فقط من خلال ال cellular
+
+342
+00:27:56,230 --> 00:27:59,490
+respiration او ال aerobic respiration زي ال
+
+343
+00:27:59,490 --> 00:28:04,650
+obligate aerobic لأنها تحتاج للنمو تحتاج ال oxygen
+
+344
+00:28:04,650 --> 00:28:08,550
+لكن بكمية قليلة أقل مما تحتاج ال obligate aerobic
+
+345
+00:28:08,550 --> 00:28:14,350
+بكتيريا عشان هيك بشوفها أقلبقليل من مش على السطح
+
+346
+00:28:14,350 --> 00:28:21,170
+يعني أقل من السطح شوية لتحت من السطح بتنمو هي
+
+347
+00:28:21,170 --> 00:28:24,390
+aerobic فقط مش anaerobic موجود فيها كل الانزيمات
+
+348
+00:28:24,390 --> 00:28:28,730
+للتخلص من ال toxic form أمثلة على ال obligate
+
+349
+00:28:28,730 --> 00:28:32,030
+aerobic bacteria البصلص والصدومونص الفكولتات of
+
+350
+00:28:32,030 --> 00:28:35,250
+anaerobic bacteria كل ال enterobacterias بما فيهم
+
+351
+00:28:35,250 --> 00:28:39,780
+ال E.coli والستفيلوكوكسبالنسبة للـ Obligate
+
+352
+00:28:39,780 --> 00:28:43,080
+Anaerobic Bacteria عندي الـ Clostridium، الـ
+
+353
+00:28:43,080 --> 00:28:46,500
+Aerotolerant Anaerobic Bacteria الـ Lactobacillus،
+
+354
+00:28:46,500 --> 00:28:49,940
+الـ Micro Aerophilus اللي هي الـ Campylobacter و
+
+355
+00:28:49,940 --> 00:28:53,780
+الـ Helicobacter Biliary هذا بالنسبة للجدول طبعا
+
+356
+00:28:53,780 --> 00:28:58,660
+بالنسبة ل الأمثلة مطلوب منكوا حفظ الأمثلة ومهمين
+
+357
+00:28:58,660 --> 00:29:04,760
+هدال الأمثلةهذا تقريبا الجدول ملخص لكل اللي ذكرناه
+
+358
+00:29:04,760 --> 00:29:10,540
+عن تصنيفات البكتيريا حسب حاجتها للأكسجين طيب هلأ
+
+359
+00:29:10,540 --> 00:29:17,640
+نيجي للخطوات عشان أعرف شو تصنيفات البكتيريا حسب
+
+360
+00:29:17,640 --> 00:29:22,480
+حاجتها للأكسجين بقوللي أني أنا بستخدم ميديا brand
+
+361
+00:29:22,480 --> 00:29:26,700
+hard infusion agar في ندي إبروت لكن أنا بستخدم ال
+
+362
+00:29:26,700 --> 00:29:30,960
+brand hard infusion agarمكوناتها بيبتون mixed
+
+363
+00:29:30,960 --> 00:29:34,220
+chard البيبتون بيكون هو عبارة عن بروتين مصدر
+
+364
+00:29:34,220 --> 00:29:38,660
+للكربون والنيتروجين اللازم لنمو البكتيريا بيف hard
+
+365
+00:29:38,660 --> 00:29:42,380
+infusion طبعا بيف اللي هو اللحم البقري Gulf Brain
+
+366
+00:29:42,380 --> 00:29:47,280
+Infusion، Gulf يعني جل Sodium Chloride طبعا عشان
+
+367
+00:29:47,280 --> 00:29:51,860
+أحافظ على ال Osmotic Pressure Dipotassium
+
+368
+00:29:51,860 --> 00:29:55,000
+Phosphate اللي هو ال buffer عشان يعادل ال BH
+
+369
+00:29:55,000 --> 00:30:01,480
+Textrose طبعا اللي هو ال nutrientاللي هو السكر في
+
+370
+00:30:01,480 --> 00:30:05,640
+عندي اكيد بما انه هي agar اكيد هيكون نسبة agar 1.5
+
+371
+00:30:05,640 --> 00:30:10,920
+% عشان اعمل اللي هو او اشوف تصنيفات البكتيريا حسب
+
+372
+00:30:10,920 --> 00:30:17,360
+حاجتها للأكسجين بقوللي هتستخدمي اتيوبات او تلاتة
+
+373
+00:30:17,360 --> 00:30:23,040
+من brain heart infusion agar دي هستخدم اتيوبات
+
+374
+00:30:23,040 --> 00:30:26,860
+الاتيوبات هي هيكون مصبوب فيها media ل brain heart
+
+375
+00:30:26,860 --> 00:30:32,750
+infusion agarهصبها بـ deep هتكون deep اللي هو مش
+
+376
+00:30:32,750 --> 00:30:35,890
+هعملها طبعا بشكل مائل فقط بعد ما تطلع من الـ
+
+377
+00:30:35,890 --> 00:30:41,190
+autoclave هخليها تتصلب بشكل عادي بشكل مستقيم بدي
+
+378
+00:30:41,190 --> 00:30:45,050
+hot plate water bath ice water bath و thermometer
+
+379
+00:30:45,050 --> 00:30:52,550
+طيب شو الخطوات بقوللي بتجيبي اللي هو ال brain
+
+380
+00:30:52,550 --> 00:30:57,680
+heart infusion agar dips tubeهدول طبعا بعد ما انت
+
+381
+00:30:57,680 --> 00:31:01,860
+جهزتيهم و حضرت ال media و طبعا طلعتيهم من ال
+
+382
+00:31:01,860 --> 00:31:05,660
+Octoclav اتصلبوا و قلنا بنحط هاي ليتيوبات في
+
+383
+00:31:05,660 --> 00:31:11,020
+boiling water bath بنجهز water bath على درجة حرارة
+
+384
+00:31:11,020 --> 00:31:15,420
+اكتر من 100 طبعا boiling water bath يعني ان ال
+
+385
+00:31:15,420 --> 00:31:18,200
+water bath بتكون الميه بتغلي يعني درجة الحرارة
+
+386
+00:31:18,200 --> 00:31:23,330
+اكتر من 100بُلي after the brain hard infusion أجار
+
+387
+00:31:23,330 --> 00:31:27,970
+ملت أكيد بما أنه درجة الحرارة مية أكيد الأجار قلنا
+
+388
+00:31:27,970 --> 00:31:32,670
+بتصلب عند 85 درجة سليسيا فأكيد هيكون هيتصلب اللي
+
+389
+00:31:32,670 --> 00:31:38,360
+هو الأجار في هاي ال mediaبقول لي لما اتأكد انه ال
+
+390
+00:31:38,360 --> 00:31:44,800
+agar اللي هو داب او sorry هو طبعا بت بيدوب عند
+
+391
+00:31:44,800 --> 00:31:49,980
+درجة حرارة 85 في الدرجة السيليزياس بيدوب عند 85
+
+392
+00:31:49,980 --> 00:31:56,160
+درجة السيليزياس و بتصلب عند 45 فبالتالي اكيد عند
+
+393
+00:31:56,160 --> 00:32:01,100
+بما انه درجة الحرارة ال water bath مية اكيد هي هي
+
+394
+00:32:01,100 --> 00:32:07,800
+دوب هذا ال agarبقولي لما اتأكد ان الأجار داب بتخلي
+
+395
+00:32:07,800 --> 00:32:12,160
+اللي هم الليتيوبات في الراك او بتخلي ال water
+
+396
+00:32:12,160 --> 00:32:18,800
+يسيله boiling انه يغلي لمدة خمس دقائق تمام اقل
+
+397
+00:32:18,800 --> 00:32:24,390
+شيخمس دقائق ليش هذا الاشي ليش انا بخلي انهبحط
+
+398
+00:32:24,390 --> 00:32:30,670
+ليتيوبات و بخليه يغلي او بخلي خمس دقايق انه يتعرض
+
+399
+00:32:30,670 --> 00:32:35,410
+لدرجة حرارة عالية بقولي عشان اضمن انه تم طرد
+
+400
+00:32:35,410 --> 00:32:40,310
+الاكسجين من ليتيوب اذا كان فيه اي غازات اي اكسجين
+
+401
+00:32:40,310 --> 00:32:44,210
+فيه ليتيوبات يتم طردها باستخدام درجة الحرارة
+
+402
+00:32:44,210 --> 00:32:48,350
+العالية بقولي بعدين بتحطي طبعا thermometer في ال
+
+403
+00:32:48,350 --> 00:32:53,220
+water bath عشان نستراق بدرجة الحرارةلما طبعا انت
+
+404
+00:32:53,220 --> 00:32:58,340
+بتقللي درجة الحرارة او بتطفي الحرارة لما توصل
+
+405
+00:32:58,340 --> 00:33:02,160
+لدرجة حرارة خمسة و أربعين درجة سيريزيا اللي قلنا
+
+406
+00:33:02,160 --> 00:33:08,220
+بتصلب عندها الوات الأجار بقولي بتقومي لتيوبات من
+
+407
+00:33:08,220 --> 00:33:14,040
+ال water path وهنا لازم أنتبه انه ما احرق اللي هو
+
+408
+00:33:14,040 --> 00:33:18,200
+لتيوب عشان مايصير اي اختلاط ويدخل ال oxygen من
+
+409
+00:33:18,200 --> 00:33:24,210
+جديدبعد ما انا رفعت ال tube من ال water bath بقولي
+
+410
+00:33:24,210 --> 00:33:28,330
+بتعملي inculate each culture into separate agarde
+
+411
+00:33:28,330 --> 00:33:34,690
+كيف انا بدي اعمل inculate في تيوبات agarde من خلال
+
+412
+00:33:34,690 --> 00:33:37,970
+اللي هو ال stabbing بدي اعمل ال stabbing باستخدام
+
+413
+00:33:37,970 --> 00:33:42,130
+ال inculating needle بقولي حاولي انك تتجنبي ال
+
+414
+00:33:42,130 --> 00:33:47,460
+shaking او انه تعملي air bubbles في ال mediaبعد ما
+
+415
+00:33:47,460 --> 00:33:51,780
+اتخلصى على طول بتحطيهم فى ال incubation تمام او
+
+416
+00:33:51,780 --> 00:33:56,360
+قبل فى ال incubation بقولى بتحطيهم فى ال ice water
+
+417
+00:33:56,360 --> 00:34:01,860
+bath ليش عشان اضمن انه الاجار اتصلب completely
+
+418
+00:34:01,860 --> 00:34:05,000
+solid until they are completely solid عشان اضمن
+
+419
+00:34:05,000 --> 00:34:10,330
+انه الاجار اتصلبانكيوبات دي بعضها بتاخد ال tube
+
+420
+00:34:10,330 --> 00:34:14,770
+بتعمليلهم انكيوباشين على درجة حرارة 37 درجة
+
+421
+00:34:14,770 --> 00:34:22,770
+سيليسيوس لمدة 24 48 و 72 ساعة ليش 48 او 72 ساعة
+
+422
+00:34:22,770 --> 00:34:27,550
+لأن البكتيريا الهوائية بتنمو أسرع من اللي هوائية
+
+423
+00:34:27,550 --> 00:34:32,830
+البكتيريا اللي هوائية عشان تنمو بتحتاج من 48 ل 72
+
+424
+00:34:32,830 --> 00:34:39,090
+ساعةبعد ما اتخلص المدة ال incubation بقولي بتشوفي
+
+425
+00:34:39,090 --> 00:34:44,990
+اللي هو نمو البكتيريا هلاحظ ان ال obligate aerobic
+
+426
+00:34:44,990 --> 00:34:48,890
+bacteria هتنمو فقط على الصدح اللي سميناها اللي هي
+
+427
+00:34:48,890 --> 00:34:52,450
+ال bellicle ال faculty of anaerobic bacteria قلنا
+
+428
+00:34:52,450 --> 00:34:56,910
+هتنمو في كل تيوب لكن اكتر شئ هشوفه على الصدح ال
+
+429
+00:34:56,910 --> 00:35:00,270
+obligate anaerobic bacteria فقط في البطم اللي هي
+
+430
+00:35:00,270 --> 00:35:04,240
+ال sedimentالـ Aerotolerant anaerobic بكتيريا قلنا
+
+431
+00:35:04,240 --> 00:35:09,900
+هيكون طبعا في كل tube لكن أكتر شئ هيكون تحت لأن هي
+
+432
+00:35:09,900 --> 00:35:16,640
+بالاصل لا هوائية ال micro aerophilic بكتيريا اللي
+
+433
+00:35:16,640 --> 00:35:21,600
+هي قلنا بتنمو في وجود ال oxygen لكن بتحتاج كمية
+
+434
+00:35:21,600 --> 00:35:26,520
+قليلة فبالتالي هتكون أسفل من أسفل السطح بشوية هذا
+
+435
+00:35:26,520 --> 00:35:31,100
+بالنسبة لتصنيفات البكتيريا حسب حاجتها لل oxygen
+
+436
+00:35:36,550 --> 00:35:40,150
+الان يوربك بكتيريا كيف بدي أنمي البكتيريا
+
+437
+00:35:40,150 --> 00:35:44,190
+اللاهوائية في عندي أكتر من طريقة عشان أنمي الان
+
+438
+00:35:44,190 --> 00:35:47,490
+يوربك بكتيريا ونلاحظ ان الان يوربك بكتيريا بتنمو
+
+439
+00:35:47,490 --> 00:35:52,950
+في الأسفل اللي هي سميناها ال sedimentفي هاي ال
+
+440
+00:35:52,950 --> 00:35:56,830
+slide ذاكر اللي هو او عامل مقارنة بين الـ
+
+441
+00:35:56,830 --> 00:36:00,470
+anaerobic bacteria و ال aerobic or facultative
+
+442
+00:36:00,470 --> 00:36:05,930
+anaerobic bacteria بالنسبالي ال anaerobic bacteria
+
+443
+00:36:05,930 --> 00:36:11,970
+بقولي ال oxygen طبعا طبعا انا في عندي toxic او في
+
+444
+00:36:11,970 --> 00:36:16,870
+عندي اللي هو toxic form لال oxygen قلنا همهي كل
+
+445
+00:36:16,870 --> 00:36:19,930
+الـ toxic form بقوللي في الـ anaerobic bacteria ما
+
+446
+00:36:19,930 --> 00:36:26,810
+بقدر اتخلص من الـ toxic form للأكسجين no detoxify
+
+447
+00:36:26,810 --> 00:36:31,050
+pathway مافي عندي أي طرق لإزالة سمية الـ toxic
+
+448
+00:36:31,050 --> 00:36:35,190
+form of أكسجين فبالتالي البكتيريا هتموت لكن في الـ
+
+449
+00:36:35,190 --> 00:36:38,450
+anaerobic bacteria و الـ facultative anaerobic
+
+450
+00:36:38,450 --> 00:36:42,350
+bacteria الـ toxic form of أكسجين بيتم إزالة
+
+451
+00:36:42,350 --> 00:36:45,980
+السمية عن طريق الإنزيماتالتالي ال super oxide
+
+452
+00:36:45,980 --> 00:36:51,040
+ديسميوتاز الكتلاز والبروكسداز فبالتالي بيتكون non
+
+453
+00:36:51,040 --> 00:36:55,800
+-toxic form اللي هي ال water و ال oxygen البكتيريا
+
+454
+00:36:55,800 --> 00:37:01,520
+بتقدر تعيش طيب كيف أنا بدي أنمي البكتيريا اللي لا
+
+455
+00:37:01,520 --> 00:37:06,640
+هوائية أول طريقة عندي اللي هي ال fluid
+
+456
+00:37:06,640 --> 00:37:11,760
+thioglycolate broth بقولي ال fluid thioglycolate
+
+457
+00:37:11,760 --> 00:37:16,230
+broth is a reducing mediareducing media ليش
+
+458
+00:37:16,230 --> 00:37:20,290
+reducing media بقول لي لأنه بتحتوي على ال Sodium
+
+459
+00:37:20,290 --> 00:37:25,870
+Thioglycolate هذا Sodium Thioglycolate أحد مكونات
+
+460
+00:37:25,870 --> 00:37:30,350
+ال media ال fluid Thioglycolate براسل شو وظيفته
+
+461
+00:37:30,350 --> 00:37:35,380
+removes O2 from the media؟بيزيل ال oxygen الموجود
+
+462
+00:37:35,380 --> 00:37:38,780
+في ال media فبالتالي هيك بقدر أنمي البكتيريا
+
+463
+00:37:38,780 --> 00:37:43,060
+لهوائية يبقى عرفنا ليش هي تعتبر reducing media
+
+464
+00:37:43,060 --> 00:37:46,580
+لأنها بتحتوي على ال sodium-thiozoloycolate اللي
+
+465
+00:37:46,580 --> 00:37:50,280
+بيزيل ال oxygen من ال media فبالتالي بوفر ظروف
+
+466
+00:37:50,280 --> 00:37:54,500
+لهوائية هذه ال media بتدعم ال aerobic and
+
+467
+00:37:54,500 --> 00:37:59,410
+anaerobic بكتيريابقول لي في هاي ال media فيه كاشف
+
+468
+00:37:59,410 --> 00:38:03,890
+هذا الكاشف عشان أعرف هل تم التخلص من ال oxygen ولا
+
+469
+00:38:03,890 --> 00:38:12,710
+لأ Rezazarin is an oxidation reduction indicator
+
+470
+00:38:12,710 --> 00:38:16,650
+that turned to pink in the presence of O2 هذا ال
+
+471
+00:38:16,650 --> 00:38:21,270
+indicator بتحول للون ال pink في حال وجود ال oxygen
+
+472
+00:38:21,270 --> 00:38:26,530
+يبقى في حال وجود ال oxygen بتحول للونالبنك هيك
+
+473
+00:38:26,530 --> 00:38:33,030
+بعرف انه والله انه ال oxygen dial موجود في هاي ال
+
+474
+00:38:33,030 --> 00:38:37,610
+media لم يتم التخلص منه بقولي small amount of agar
+
+475
+00:38:37,610 --> 00:38:41,690
+في عندى كمية قليلة موجودة في ال media to retard
+
+476
+00:38:41,690 --> 00:38:47,300
+gasesليش موجود في عندي نسبة أجار أو أجار بنسبة
+
+477
+00:38:47,300 --> 00:38:53,640
+قليلة جدا لأن الأجار بيعمل Trapping بيحجز البخار
+
+478
+00:38:53,640 --> 00:38:59,420
+وبيحجز الغازات والأكسوجين فبالتالي بيمنع انتشارها
+
+479
+00:38:59,420 --> 00:39:04,640
+في Lithium وبيوفر هيك ظروف لا هوائية فيها المعلومة
+
+480
+00:39:05,000 --> 00:39:09,560
+إنه أنا الـ Thioglycolate breath بروح أنا بسخّنها
+
+481
+00:39:09,560 --> 00:39:13,340
+عشان أطرد أي غازات موجودة في الـ medium إحنا قلنا
+
+482
+00:39:13,340 --> 00:39:18,520
+قبل شوية في كيف أنا بدي أوفر ظروف أو أشوف كيف
+
+483
+00:39:18,520 --> 00:39:22,160
+تصنيفات البكتيريا حسب حاجتها للـ Oxygen قلنا بعد
+
+484
+00:39:22,160 --> 00:39:29,840
+ما أنا أخد اللي هو أحط التيوبات في ال water bath
+
+485
+00:39:29,840 --> 00:39:31,900
+لما أتأكد إنه
+
+486
+00:39:37,270 --> 00:39:42,710
+عشان اضمن ان كل الغازات اللى موجودة في ال media تم
+
+487
+00:39:42,710 --> 00:39:47,310
+طردها هكذا انا وفر الظروف لهوائية هذا بالنسبة ل
+
+488
+00:39:47,740 --> 00:39:52,660
+الأول طريقة لتنمية البكتيريا اللاهوائية لـ Fluid
+
+489
+00:39:52,660 --> 00:39:56,400
+Thioglycolate Broth هي Reducing Media وقلنا ليش
+
+490
+00:39:56,400 --> 00:39:59,720
+Reducing Media؟ شو الـ Indicator الموجود في هذه
+
+491
+00:39:59,720 --> 00:40:05,300
+الـ Media؟ طبعا موجود أكيد نسبة أجار قليلة عشان
+
+492
+00:40:05,300 --> 00:40:09,300
+تعمل Trapping للغازات والأكسجين وأفهر ظروف
+
+493
+00:40:09,300 --> 00:40:14,990
+لاهوائيةتاني طريقة عشان انا اوفر ظروف لاهوائية الـ
+
+494
+00:40:14,990 --> 00:40:20,190
+gas bag system الـ anaerobic jar anaerobic jar
+
+495
+00:40:20,190 --> 00:40:24,150
+such as the gas bag are visible in which an
+
+496
+00:40:24,150 --> 00:40:28,310
+anaerobic environment بقوللي هو عشان اوفر ظروف
+
+497
+00:40:28,310 --> 00:40:32,490
+لاهوائية هذه الظروف اللاهوائية كيف انا بوفرها من
+
+498
+00:40:32,490 --> 00:40:37,010
+خلال is generated after inoculate media are sealed
+
+499
+00:40:37,010 --> 00:40:43,270
+into the chamberبقول لي ان الان european jar هو
+
+500
+00:40:43,270 --> 00:40:48,410
+عبارة عن مرتبان محكم الإغلاق sealed يعني محكم
+
+501
+00:40:48,410 --> 00:40:55,360
+الإغلاقبجيب البكتيريا اللى انا نمتها او اللى انا
+
+502
+00:40:55,360 --> 00:40:59,700
+بالاول زرعتها طبعا زرعت بكتيريا على ميديا high
+
+503
+00:40:59,700 --> 00:41:04,000
+البكتيريا بتحتاج بروفلا هوائية زرعتها على اطباق
+
+504
+00:41:04,000 --> 00:41:08,880
+بروح بحط الانيكلاتي ميديا الميديا المزروعة في هذا
+
+505
+00:41:08,880 --> 00:41:14,700
+ال chamber و بسكره طبعا قبل ما انا اسكره بقوللي في
+
+506
+00:41:14,700 --> 00:41:21,360
+في هذا الجار يكوناللي هو الـ Gas Generator
+
+507
+00:41:21,360 --> 00:41:26,600
+Envelope إيش هذا الـ Gas Generator Envelope عبارة
+
+508
+00:41:26,600 --> 00:41:32,800
+عن كيس هذا الكيس موجود فيه مواد كيميائية تمام يبقى
+
+509
+00:41:32,800 --> 00:41:36,280
+موجود عبارة اللي بالنسبة لألان الـ Aerobic Jar
+
+510
+00:41:36,280 --> 00:41:41,180
+عبارة عن مرتبان بروح أنا بحط الأطباق اللي أنا زرعت
+
+511
+00:41:41,180 --> 00:41:44,980
+البكتيريا اللي بتحتاج في ظروف لاهوائية بحطها
+
+512
+00:41:44,980 --> 00:41:49,880
+upside down في هذا الجرعلى جانب بحط كيس اللى هو ال
+
+513
+00:41:49,880 --> 00:41:54,960
+gas generator generator envelope هذا الكيس موجود
+
+514
+00:41:54,960 --> 00:42:00,700
+فيه مواد كيميائية بروح بضيف عليه water تمام بضيف
+
+515
+00:42:00,700 --> 00:42:03,800
+عليه ميه قبل ما انا طبعا انا اول اشي بفتح الكيس
+
+516
+00:42:03,800 --> 00:42:09,100
+بضيف عليه ميه بكون حتى الأطباق upside down و بعدين
+
+517
+00:42:09,100 --> 00:42:13,880
+بروح بسكر اللى هو هذا المرتبان بيكون لازم يكون
+
+518
+00:42:13,880 --> 00:42:19,900
+محكم الإغلاق بقول ليالـ chemicals طبعا مكملها اللي
+
+519
+00:42:19,900 --> 00:42:23,060
+هو ال gas generator envelope that are placed in
+
+520
+00:42:23,060 --> 00:42:28,320
+the jar just prior to sailing طبعا احنا بنحطه قبل
+
+521
+00:42:28,320 --> 00:42:33,040
+ما انا اسكر المرتبان روحت سكرت المرتبان بقولي ال
+
+522
+00:42:33,040 --> 00:42:35,920
+chemicals in the envelope قولنا في مواد كيميائية
+
+523
+00:42:35,920 --> 00:42:41,020
+في هذا الكيس بقولي بتنتج انا ضفت ال water طبعا
+
+524
+00:42:41,020 --> 00:42:47,540
+فتحته ضفت ال water عشان يحفظ التفاعلبقول لي المواد
+
+525
+00:42:47,540 --> 00:42:50,920
+الكيميائية بيصير في اندي تفاعل كيميائي بينتج
+
+526
+00:42:50,920 --> 00:42:55,600
+hydrogen gas and carbon dioxide بعد ما انا سكرت ال
+
+527
+00:42:55,600 --> 00:43:00,220
+chamber هذا المرتبان صار تفاعل نواتج التفاعل ال
+
+528
+00:43:00,220 --> 00:43:03,740
+hydrogen gas و ال carbon dioxide بقوللي ال
+
+529
+00:43:03,740 --> 00:43:08,620
+hydrogen gas اللي نتج من التفاعل بيرتبط مع ال free
+
+530
+00:43:08,620 --> 00:43:13,460
+oxygen الموجود في ال chamberيبقى الـ hydrogen gas
+
+531
+00:43:13,460 --> 00:43:16,260
+اللى ناتج من التفاعل بيرتبط مع ال oxygen اللى
+
+532
+00:43:16,260 --> 00:43:19,800
+موجود في ال chamber هيك أنا اتخلصت من ال oxygen
+
+533
+00:43:19,800 --> 00:43:24,040
+اللى موجود في ال chamber هينتج في النهاية hydrogen
+
+534
+00:43:24,040 --> 00:43:30,760
+gas مع ال oxygen هينتج مع اندي water يبقى بلاحظ
+
+535
+00:43:30,760 --> 00:43:34,560
+انه او عشان استدل انه صار في اندي ظروف لا هوائية
+
+536
+00:43:34,560 --> 00:43:39,300
+من خلال تعرق الجدار لأنه بتكون عندى waterبقول الـ
+
+537
+00:43:39,300 --> 00:43:43,640
+carbon الـ carbon dioxide اللي نتج من التفاعل is
+
+538
+00:43:43,640 --> 00:43:46,480
+required for the growth of certain organisms في
+
+539
+00:43:46,480 --> 00:43:51,600
+بعض أنواع البكتيريا بتحتاج الـ CO2 لانمو مثليني
+
+540
+00:43:51,600 --> 00:43:55,000
+blue indicator strip في عندي أنا strip مثليني blue
+
+541
+00:43:55,000 --> 00:44:00,160
+indicator strip موجودة في هذا الجار usually placed
+
+542
+00:44:00,160 --> 00:44:03,620
+in the jar it turned colorless when the oxygen has
+
+543
+00:44:03,620 --> 00:44:08,470
+been removedفي هذا الطريقة في عندي الـ methylene
+
+544
+00:44:08,470 --> 00:44:13,170
+blue indicator strip بقول لي بيصير لونها colorless
+
+545
+00:44:13,170 --> 00:44:17,930
+لو الأكسجين تم إزالته عشان أتأكد أنه الأكسجين تم
+
+546
+00:44:17,930 --> 00:44:22,170
+إزالته وتوفر عندي ظروف لا هوائية بيصير هذا ال
+
+547
+00:44:22,170 --> 00:44:26,730
+strip لونه colorless هذا بالنسبة للـ anaerobic jar
+
+548
+00:44:26,730 --> 00:44:32,810
+هنشوفه في هاي ال slide هاي ال slide هو موضح ال
+
+549
+00:44:32,810 --> 00:44:38,470
+anaerobic jarبقول لي او اذا احنا ملاحظين في ال هذا
+
+550
+00:44:38,470 --> 00:44:43,550
+ال هاي ال slide في عندي gas generator envelope في
+
+551
+00:44:43,550 --> 00:44:47,910
+عندي case هذا ال case قولنا فيه مواد كيميائية انا
+
+552
+00:44:47,910 --> 00:44:52,610
+اول شئ بصفط الأطباق upside down تمام اللي انا
+
+553
+00:44:52,610 --> 00:44:56,370
+زراعت فيها البكتيريا بتحتاج ظروف لاهوائيةموجود هذا
+
+554
+00:44:56,370 --> 00:44:59,290
+الكيس الموجود فيه المواد الكيميائية بفتح هذا الكيس
+
+555
+00:44:59,290 --> 00:45:04,550
+بضيف ميه water is added to chemical in envelope
+
+556
+00:45:04,550 --> 00:45:11,670
+هينتج عندي ال H2 وCO2 من هذا ال gas او من هذا ال
+
+557
+00:45:11,670 --> 00:45:17,750
+envelope تمام بقوللي انه ال hydrogen gas هيرتبط مع
+
+558
+00:45:17,750 --> 00:45:21,290
+ال oxygen الموجود في ال chamber هيتكون عندي water
+
+559
+00:45:21,290 --> 00:45:25,050
+فبالتالي هيك انا قدرت اتخلص من ال oxygen الموجود
+
+560
+00:45:25,050 --> 00:45:30,400
+فيلتشامبر هيك انا وفرت ظروف لا هوائية ال carbon ال
+
+561
+00:45:30,400 --> 00:45:35,180
+carbon dioxide بقول ليه بيلزم ل growth of certain
+
+562
+00:45:35,180 --> 00:45:39,540
+microorganism هان في عندي قولنا الان ال indicator
+
+563
+00:45:39,540 --> 00:45:43,960
+strip اللي هي ال methylene blue في حال توفر عندي
+
+564
+00:45:43,960 --> 00:45:47,420
+ظروف لا هوائية في حال نقص ال oxygen or absence of
+
+565
+00:45:47,420 --> 00:45:52,530
+oxygen بيصير لونها colorlessOxygen is removed from
+
+566
+00:45:52,530 --> 00:45:55,110
+the chamber الـ Oxygen بيتم إزالته من ال chamber
+
+567
+00:45:55,110 --> 00:45:59,330
+من خلال combining with hydrogen هيرتبط مع ال
+
+568
+00:45:59,330 --> 00:46:04,750
+hydrogen اللي ناتج من التفاعل من اللي هو ال gas in
+
+569
+00:46:04,750 --> 00:46:08,390
+generator envelope to form water هيتكون عندي
+
+570
+00:46:08,390 --> 00:46:12,030
+بالنهاية water قولنا باستدل على التفاعل من خلال
+
+571
+00:46:12,030 --> 00:46:16,830
+تعرق الجدار لأنه اتكون عندي waterولهذا التفاعل
+
+572
+00:46:16,830 --> 00:46:20,210
+this reaction اللي هو ارتباط الـ H الهيدروجين جاز
+
+573
+00:46:20,210 --> 00:46:25,230
+مع الـ O2 catalyzed by the palladium pellets بيتم
+
+574
+00:46:25,230 --> 00:46:28,490
+تحفيزه باستخدام ال palladium pellets اللي بتكون
+
+575
+00:46:28,490 --> 00:46:33,250
+موجودة في ال chamber هذا بالنسبة للآن يورو بيك جار
+
+576
+00:46:33,250 --> 00:46:38,390
+طب في عندي نفس الفكرة لكن مش جار بيكون gas bag
+
+577
+00:46:38,390 --> 00:46:44,640
+powerاللي هو Case مش مرتبان بدل المرتبان بيكون
+
+578
+00:46:44,640 --> 00:46:49,420
+موجود Case نفس الفكرة بيكون في Case داخله مواد
+
+579
+00:46:49,420 --> 00:46:53,320
+كيميائية بروح أنا بفتح ال Case بضيف عليه الميه
+
+580
+00:46:53,320 --> 00:47:00,100
+بيصير تفاعل بوجود محفظ معين بيحفظ التفاعل بيرتبط
+
+581
+00:47:00,100 --> 00:47:04,140
+بالنهاية بينتج اللي هو بينتج نفس الفكرة اللي كانت
+
+582
+00:47:04,140 --> 00:47:08,770
+في الجعار بينتج H2هيرتبط مع ال oxygen اللى موجود
+
+583
+00:47:08,770 --> 00:47:13,430
+داخل هذا ال case هك بيوفر ظروف لا هوائية طبعا انا
+
+584
+00:47:13,430 --> 00:47:17,470
+بحط الأطباق في هذا ال case طبعا مش مش زى ال jar
+
+585
+00:47:17,470 --> 00:47:21,430
+بحط الأطباق فى ال case بقولك هان اخر خطوة انك انت
+
+586
+00:47:21,430 --> 00:47:28,500
+لازمطبعا تستخدمي شريط عشان او مثلا ملقط تسكري هذا
+
+587
+00:47:28,500 --> 00:47:32,620
+الكيس عشان يوفر الظروف اللي هوائية بعدين بتاخدي
+
+588
+00:47:32,620 --> 00:47:36,260
+هذا الكيس و بتحطيه في ال incubation هيك انا بوفر
+
+589
+00:47:36,260 --> 00:47:41,020
+ظروف لا هوائية نفس فكرة المرتبان لكن هو عبارة عن
+
+590
+00:47:41,020 --> 00:47:45,100
+كيس بحط الأطباق داخل الكيس و بسكر هذا الكيس
+
+591
+00:47:45,100 --> 00:47:49,360
+باستخدام sealing bar اللي هو شريط او ملقط لكن لازم
+
+592
+00:47:49,360 --> 00:47:54,450
+يكون اتأكد ان هو محكم الإغلاقهذا بالنسبة للجاز باج
+
+593
+00:47:54,450 --> 00:48:00,950
+باش اللي هو ال case نيجي ل ال candle jar ال candle
+
+594
+00:48:00,950 --> 00:48:04,750
+jar ال candle jar is used to create micro
+
+595
+00:48:04,750 --> 00:48:09,490
+aerophilic condition هذا ال candle jar يستخدم
+
+596
+00:48:09,490 --> 00:48:13,710
+لتوفير الظروف اللي هي البكتيريا أو لتنمية
+
+597
+00:48:13,710 --> 00:48:18,120
+البكتيرياالـ Micro Aerophilic Bacteria الـ Micro
+
+598
+00:48:18,120 --> 00:48:22,360
+Aerophilic Bacteria قلنا بتحتاج كمية قليلة من
+
+599
+00:48:22,360 --> 00:48:27,060
+الأكسجين للنمو شوف فكرة ال candle jar هو عبارة عن
+
+600
+00:48:27,060 --> 00:48:33,370
+مرتبان محكم الإغلاق تمام محكم الإغلاقبحط فيه اللي
+
+601
+00:48:33,370 --> 00:48:38,110
+هي ال medium البكتيريا اللي انا او الأطباق
+
+602
+00:48:38,110 --> 00:48:43,230
+البكتيريا اللي انا زرعتها على ال media على الطبق
+
+603
+00:48:43,230 --> 00:48:47,930
+اللي بتحتاج ظروف او بتحتاج جميعة قليلة من ال
+
+604
+00:48:47,930 --> 00:48:52,850
+oxygen بحطها upside down بقوللي اخر شئ بتحطي ال
+
+605
+00:48:52,850 --> 00:48:57,490
+candle بتشعلي ال candle اللي هي الشمعة ممكن احنا
+
+606
+00:48:57,490 --> 00:49:03,470
+نجيب slideبنثبتها على آخر اللي هو petri dish و
+
+607
+00:49:03,470 --> 00:49:09,070
+بشغل الشمعة بولّع الشمعة تمام و بروح أنا بسكر هذا
+
+608
+00:49:09,070 --> 00:49:12,990
+ال candle أو بسكر ال jar ال candle ال jar بسكره
+
+609
+00:49:12,990 --> 00:49:17,990
+بعد ما أنا اولّع الشمعة تمام ال candle is lit بضو
+
+610
+00:49:17,990 --> 00:49:21,190
+الشمعة and the jar is sealed و بسكر اللي هو
+
+611
+00:49:21,190 --> 00:49:25,310
+المرتبان بقول لي ال candle will burn and reduce
+
+612
+00:49:25,310 --> 00:49:30,240
+the oxygen concentration هان انهبعد ما انت تولعي
+
+613
+00:49:30,240 --> 00:49:36,420
+الشمعة وتسكري المرتبان الشمعة هتحرق كل ال Oxygen
+
+614
+00:49:36,420 --> 00:49:44,420
+الموجود في داخل الجار فبالتالي هيطلع CO2 لازم
+
+615
+00:49:44,420 --> 00:49:48,120
+لنموء البكتيريا رح يظل نسبة قليلة من ال Oxygen هيك
+
+616
+00:49:48,120 --> 00:49:52,800
+انا وفرت ظروف مناسبة لـMicro Aerophilic Bacteria
+
+617
+00:49:54,180 --> 00:49:57,700
+طبعا اسم اللى هو الطريقة ال candle jar لإني أنا
+
+618
+00:49:57,700 --> 00:50:03,400
+بستخدم candle شمعة فيهاي الطريقة فترة الحضانة طبعا
+
+619
+00:50:03,400 --> 00:50:09,700
+لا تقل عن 48 ساعة حتى تنمو البكتيريا و تتكثر
+
+620
+00:50:09,700 --> 00:50:15,140
+بالنسبة لأخر شئ اللى هو ال CO2 generating packet
+
+621
+00:50:19,700 --> 00:50:23,140
+طبعا هذا عشان اوفر ظروف الـ CO2 بعض أنواع
+
+622
+00:50:23,140 --> 00:50:27,820
+البكتيريا قلنا بتحتاج للـ CO2 لانمو نفس الفكرة في
+
+623
+00:50:27,820 --> 00:50:32,100
+بكون عندي envelope تمام بيوجد داخله gas generator
+
+624
+00:50:32,100 --> 00:50:37,940
+عشان اللي هو يكون عندي الجاز بحط ال culture
+
+625
+00:50:37,940 --> 00:50:41,340
+البكتيريا في هذا ال case طبعا هان Hyperbolic
+
+626
+00:50:41,340 --> 00:50:41,640
+Hyperbolic Hyperbolic Hyperbolic Hyperbolic
+
+627
+00:50:41,640 --> 00:50:42,000
+Hyperbolic Hyperbolic Hyperbolic Hyperbolic
+
+628
+00:50:42,000 --> 00:50:43,180
+Hyperbolic Hyperbolic Hyperbolic Hyperbolic
+
+629
+00:50:43,180 --> 00:50:43,560
+Hyperbolic Hyperbolic Hyperbolic Hyperbolic
+
+630
+00:50:43,560 --> 00:50:43,780
+Hyperbolic Hyperbolic Hyperbolic Hyperbolic
+
+631
+00:50:43,780 --> 00:50:43,780
+Hyperbolic Hyperbolic Hyperbolic Hyperbolic
+
+632
+00:50:43,780 --> 00:50:43,840
+Hyperbolic Hyperbolic Hyperbolic Hyperbolic
+
+633
+00:50:43,840 --> 00:50:47,890
+Hyperbolic Hyperbolic Hyperbهكذا نتخلص من الـ O2
+
+634
+00:50:47,890 --> 00:50:52,950
+ونحصل على الـ CO2 اللازم للنمو من خلال الـ Gas
+
+635
+00:50:52,950 --> 00:50:59,790
+Generator طبعا هذا يختص بالبكتيريا الهوائية اخر
+
+636
+00:50:59,790 --> 00:51:02,790
+اشي في هذا ال chapter اللي هو الـ anaerobic
+
+637
+00:51:02,790 --> 00:51:09,470
+chamber بلي هذا ال anaerobic chamber provide
+
+638
+00:51:09,470 --> 00:51:15,640
+a strict anaerobic atmosphericعشان انا اوفر ظروف
+
+639
+00:51:15,640 --> 00:51:21,300
+لهوائية طبعا باستخدام اللي هو palladium catalyst
+
+640
+00:51:21,300 --> 00:51:27,060
+and hydrogen gas mixed of 5%طبعا في عندي بيكون
+
+641
+00:51:27,060 --> 00:51:34,080
+اللي هو chamber او زي container او chamber كبير
+
+642
+00:51:34,080 --> 00:51:38,100
+اذا ما نلاحظ في هذه الصورة كل الإدوات موجودة داخل
+
+643
+00:51:38,100 --> 00:51:43,740
+هذا ال chamber بدخل فقط او اني انا بشتغل من خلال
+
+644
+00:51:43,740 --> 00:51:46,360
+قفازات متطيئة زي ما ملاحظين في هذه الصورة في عندي
+
+645
+00:51:46,360 --> 00:51:50,580
+أنا قفازات من خلال هذه القفازات انا بقدر اشتغل
+
+646
+00:51:50,580 --> 00:51:55,350
+داخل هذا ال chamberهك بيوفر طبعا او بمنع دخول ال
+
+647
+00:51:55,350 --> 00:51:59,710
+oxygen لداخل ال chamber بالنسبة للظروف اللي
+
+648
+00:51:59,710 --> 00:52:04,130
+لاهوائية كيف بتتكون طبعا في انديهيدروجين جاز بيصير
+
+649
+00:52:04,130 --> 00:52:07,190
+تفاعل بين ال hydrogen gas و ال oxygen اللي موجود
+
+650
+00:52:07,190 --> 00:52:12,010
+داخل ال chamber هك بتخلص من اللي هو ال oxygen بوفر
+
+651
+00:52:12,010 --> 00:52:16,410
+ظروف CO2 بالتالي بتتوفر الظروف اللي لاهوائية
+
+652
+00:52:17,720 --> 00:52:26,880
+الباليديوم كان مهم كمحفز لإرتباط الـH2 مع الأكسجين
+
+653
+00:52:26,880 --> 00:52:31,740
+عشان أتخلص من الأكسجين والذي يتكون عندى الـH2O
+
+654
+00:52:31,740 --> 00:52:37,740
+هكذا نكون خلصنا الـchamber يعطيكم العافية والسلام
+
+655
+00:52:37,740 --> 00:52:39,680
+عليكم ورحمة الله وبركاته
+
diff --git a/PL9fwy3NUQKwZWgAb_V1ATlRRsH-6i3cPC/UNVvGjbAdO4_postprocess.srt b/PL9fwy3NUQKwZWgAb_V1ATlRRsH-6i3cPC/UNVvGjbAdO4_postprocess.srt
new file mode 100644
index 0000000000000000000000000000000000000000..8af180fd346885e209807267034f85c424dd5e75
--- /dev/null
+++ b/PL9fwy3NUQKwZWgAb_V1ATlRRsH-6i3cPC/UNVvGjbAdO4_postprocess.srt
@@ -0,0 +1,1064 @@
+1
+00:00:00,250 --> 00:00:04,010
+السلام عليكم ورحمة الله وبركاته نستكمل معاكم
+
+2
+00:00:04,010 --> 00:00:07,410
+محاضرات مساق الـ Microbiology العملية في هذا
+
+3
+00:00:07,410 --> 00:00:10,470
+اللقاء هتكون المحاضرة بعنوان الـ Simple Stain
+
+4
+00:00:10,470 --> 00:00:14,310
+هنتعرف على الصبغات وأنواع الصبغات اللي من ضمنها
+
+5
+00:00:14,310 --> 00:00:17,990
+الـ Simple Stain في المعمل السابق اتعرفنا أن
+
+6
+00:00:17,990 --> 00:00:22,490
+البكتيريا حجمها صغير لاترى بالعين المجردة يتراوح
+
+7
+00:00:22,490 --> 00:00:27,800
+حجمها ما بين مصر ل2 ميكرو مترعشان أتغلب على هذه
+
+8
+00:00:27,800 --> 00:00:31,020
+المشكلة استخدمت الميكروسكوب عشان يعملي
+
+9
+00:00:31,020 --> 00:00:34,940
+magnification تكبيرني أنا أقدر أشوفها من الصفات
+
+10
+00:00:34,940 --> 00:00:38,300
+الأخرى رأى البكتيريا إنها transparent شفافة
+
+11
+00:00:38,300 --> 00:00:42,440
+colorless ما إلها لون featureless ما إلها أي معالم
+
+12
+00:00:42,440 --> 00:00:46,920
+عشان أتغلب على هذه المشاكل استخدمت لستان استخدمت
+
+13
+00:00:46,920 --> 00:00:51,320
+الصبغات حتى أقدر أميزها بشكل واضح و أتعرف على
+
+14
+00:00:51,320 --> 00:00:56,600
+شكلها طيب لو بدنا نعرف لستان الصبغاتWhat is a
+
+15
+00:00:56,600 --> 00:01:00,260
+stain? A stain is a substance that adhere to a
+
+16
+00:01:00,260 --> 00:01:04,480
+cell giving the cell color هي مادة بتلتصق بالخلايا
+
+17
+00:01:04,480 --> 00:01:08,880
+و بتعطي الخلايا لون اتفقنا ان البكتيريا colorless
+
+18
+00:01:08,880 --> 00:01:13,300
+من خلال الستان هتصير color تعطيها لون وجود هذا
+
+19
+00:01:13,300 --> 00:01:16,560
+اللون the presence of color give the cell
+
+20
+00:01:16,560 --> 00:01:20,650
+significant contrast so are much more visibleوجود
+
+21
+00:01:20,650 --> 00:01:25,290
+هذا اللون هيجعل الخلايا أكثر تباينا واضوحا وسهل
+
+22
+00:01:25,290 --> 00:01:30,650
+رؤيتها و التعرف علي شكلها طيب لو احنا سألنا كيف
+
+23
+00:01:30,650 --> 00:01:35,010
+الستان كيف الصبغة بتلتسق بالخلايا بال cell او ليش
+
+24
+00:01:35,010 --> 00:01:39,870
+الصبغة بتصبغ البكتيريا وما بتصبغ أشياء في العيلة
+
+25
+00:01:39,870 --> 00:01:43,610
+بقولنا ان في عندي قوة ارتباط affinity ما بين
+
+26
+00:01:43,610 --> 00:01:48,050
+الستان وما بين ال cell طيب شو سبب قوة الارتباط هاي
+
+27
+00:01:48,960 --> 00:01:54,260
+احنا بنعرف أنه البكتيريا فيها مكونات هذه المكونات
+
+28
+00:01:54,260 --> 00:01:58,940
+في بعضها بيحمل شحنة موجبة والبعض الآخر بيحمل شحنة
+
+29
+00:01:58,940 --> 00:02:03,360
+سالبة لكن الشحنة الكلية للبكتيريا ال over all
+
+30
+00:02:03,360 --> 00:02:08,320
+charge تعتبر negative charge شحنة سالبة واحنا
+
+31
+00:02:08,320 --> 00:02:13,690
+عارفين أن الشحنات المختلفة تتجذبعشان يصير تجاذب ما
+
+32
+00:02:13,690 --> 00:02:17,670
+بين السل، ما بين الخلايا، ما بين البكتيريا وما بين
+
+33
+00:02:17,670 --> 00:02:23,650
+الصبغة للستان لازم تكون شحنة الصبغة شحنة مخالفة
+
+34
+00:02:23,650 --> 00:02:28,590
+لشحنة البكتيريا يبقى لازم تكون شحنة الصبغة شحنة
+
+35
+00:02:28,590 --> 00:02:33,590
+موجبة يعني basic stain بالتالي بيصير في عندي تجاذب
+
+36
+00:02:33,590 --> 00:02:37,900
+وارتباط وبتلتصق الصبغة على الخلايايبقى شحنة
+
+37
+00:02:37,900 --> 00:02:42,240
+البكتيريا سالبة عشان هي أنا بستخدم صبغات موجة بت
+
+38
+00:02:42,240 --> 00:02:48,060
+الشحنة صبغات basic stain طب لو عندي أنا organism
+
+39
+00:02:48,060 --> 00:02:52,060
+غير البكتيريا شحنة الكلية موجة بقى شو هستخدم
+
+40
+00:02:52,060 --> 00:02:57,660
+هستخدم صبغات سالبة الشحنة هستخدم acidic stain يبقى
+
+41
+00:02:57,660 --> 00:03:00,940
+في عندي أنا different stain اتكلمنا قولنا في عندي
+
+42
+00:03:00,940 --> 00:03:04,990
+acidic stain في عندي basic stainhave different
+
+43
+00:03:04,990 --> 00:03:08,330
+affinities for different organisms اللي هقوة
+
+44
+00:03:08,330 --> 00:03:13,230
+ارتباط مختلفة بالكائنات المختلفة عندي انا ال basic
+
+45
+00:03:13,230 --> 00:03:17,410
+stand بتصبخ كائنات بتحمل شحنة سالفة ال acidic
+
+46
+00:03:17,410 --> 00:03:21,790
+stand بتصبخ كائنات بتحمل شحنة موجة يعني يبقى قوة
+
+47
+00:03:21,790 --> 00:03:26,950
+ارتباط مختلفة لكائنات مختلفة or different part of
+
+48
+00:03:26,950 --> 00:03:31,430
+organism طيب لو انا بدي أصبغ مكون داخل الخلية
+
+49
+00:03:31,430 --> 00:03:35,820
+لسبيل مثل ال proteinوقولنا حسب هذا المكون والله
+
+50
+00:03:35,820 --> 00:03:40,600
+إذا شحنت هذا المكون صالبة بصبغ بيه شحنات موجبة
+
+51
+00:03:40,600 --> 00:03:46,420
+بصبغ بيه أعفون صبغات موجبة basic stem لو شحنت هذا
+
+52
+00:03:46,420 --> 00:03:51,700
+المكون موجبة بصبغ بيه صبغات صالبة بيه acidic stem
+
+53
+00:03:51,700 --> 00:03:57,160
+there are used to differentiate different type of
+
+54
+00:03:57,160 --> 00:04:02,090
+organism or to view specific part of organismقلنا
+
+55
+00:04:02,090 --> 00:04:06,610
+إن الستان بتعطي خلايا لون وهذا بيجعل زهر رؤيتها
+
+56
+00:04:06,610 --> 00:04:10,810
+وتمييزها ويتعرف على شكلها لكن بقول في عند
+
+57
+00:04:10,810 --> 00:04:15,630
+استخدامات أخرى لاستان استخدام آخر to differentiate
+
+58
+00:04:15,630 --> 00:04:20,010
+different type of organism عشان يفرق بين أنواع
+
+59
+00:04:20,010 --> 00:04:24,710
+مختلفة من الكائنات بسمي هذا النوع من الصبغات اللي
+
+60
+00:04:24,710 --> 00:04:29,290
+مميزلة بين أنواع مختلفة من الكائنات differential
+
+61
+00:04:30,540 --> 00:04:35,620
+تمام هنتعرف عليها بالتفصيل في معامل لاحقة هاي ال
+
+62
+00:04:35,620 --> 00:04:41,760
+differential stain بتقسم ل البكتيريا ل مجموعات or
+
+63
+00:04:41,760 --> 00:04:45,320
+to view specific part of organism او اني انا اشوف
+
+64
+00:04:45,320 --> 00:04:49,480
+مكون من مكونات ال organism وهي الصبغات بسميها
+
+65
+00:04:49,480 --> 00:04:54,460
+special stain بتصبغ مكون خاص في الخلية البكتيرية
+
+66
+00:04:54,460 --> 00:04:57,260
+على سبيل المثال بدي اصبغ ال spore ال endospore
+
+67
+00:04:57,260 --> 00:05:02,520
+stain تعتبر special stain بدي اشوفمكوّن خاص داخل
+
+68
+00:05:02,520 --> 00:05:07,960
+الخلية البكتيريا هنا قلنا ان لستان بستخدمها انا
+
+69
+00:05:07,960 --> 00:05:12,520
+عشان اتعرف على شكل البكتيريا bacterial morphology
+
+70
+00:05:12,520 --> 00:05:16,860
+يبقى لستان بشكل عام تستخدم حتى ان انا اتعرف على ال
+
+71
+00:05:16,860 --> 00:05:20,800
+bacterial morphology بقولي ال bacterial morphology
+
+72
+00:05:20,800 --> 00:05:24,680
+ممكن اتعرف عليه مش بس من خلال الصبقات في طرق تانية
+
+73
+00:05:24,680 --> 00:05:29,340
+الطريقة التانية اسمها ال wet mountهذه الطريقة
+
+74
+00:05:29,340 --> 00:05:34,380
+البكتيريا اللى أنا بشوفها بتكون living بتكون حية
+
+75
+00:05:34,380 --> 00:05:40,380
+organism غير مصبوغة كيف أنا بعمل هذه الطريقة بجيب
+
+76
+00:05:40,380 --> 00:05:44,380
+slide و بحط drop of normal saline normal saline هو
+
+77
+00:05:44,380 --> 00:05:51,360
+محلول ملحي بتركيز 85% من 100% هذا المحلول بيتم
+
+78
+00:05:51,360 --> 00:05:53,080
+تحضيره بطريقة معينة
+
+79
+00:05:55,470 --> 00:05:58,850
+وبيحافظ على الـ osmotic pressure على الضغط
+
+80
+00:05:58,850 --> 00:06:02,310
+الاصمازي بعد ما انا احط drop من ال normal saline
+
+81
+00:06:02,310 --> 00:06:05,690
+بجيب touch من البكتيريا من وين بجيب ال touch من
+
+82
+00:06:05,690 --> 00:06:09,010
+البكتيريا هذا بيكون في طبعا عندي طبق petri dish
+
+83
+00:06:09,010 --> 00:06:13,230
+بطلعه من ال incubator من الحاضنة و باخد touch من
+
+84
+00:06:13,230 --> 00:06:17,190
+البكتيريا باستخدام ال loop سواء كان بلاستيك أو بال
+
+85
+00:06:17,190 --> 00:06:22,080
+نيكروم loop و لازم يكون طبعا استيرال معقبباستخدم
+
+86
+00:06:22,080 --> 00:06:26,520
+باخد البكتيريا من طبق هذا الطبق البتري داشوي
+
+87
+00:06:26,520 --> 00:06:31,980
+باستخدام ال loop بعدها بعمل mix خفيف بين ال normal
+
+88
+00:06:31,980 --> 00:06:36,740
+saline فيه وبعد ما اخلص بحط ال cover slip و تحت ال
+
+89
+00:06:36,740 --> 00:06:41,000
+microscope بشوف بكتيريا living حية لأن انا طلعت
+
+90
+00:06:41,000 --> 00:06:45,200
+البكتيريا من ال incubator تمام understand لأنه انا
+
+91
+00:06:45,200 --> 00:06:49,340
+ما استخدمت اي صبغة خلال خطوات شغلي فبالتاني مش
+
+92
+00:06:49,340 --> 00:06:53,880
+هتكون مسبوقةنفس الفكرة لما كنتوا تعملوها في ال
+
+93
+00:06:53,880 --> 00:06:57,620
+urine analysis كنتوا تعملوها centrifugation للعينة
+
+94
+00:06:57,620 --> 00:07:00,780
+تتخلصوا من ال supernatant وتاخدوا ال sediment
+
+95
+00:07:00,780 --> 00:07:04,060
+وتحطوه على ال slide و cover slip و تحت الميكروسكوب
+
+96
+00:07:04,060 --> 00:07:08,600
+بتشوفوا البكتيريا living حية لأنك انت ماعملت اي
+
+97
+00:07:08,600 --> 00:07:12,260
+خطوة قتلة البكتيريا كانت في العينة unstained
+
+98
+00:07:12,260 --> 00:07:15,440
+ماصبغتيش مستخدمتيش اي صباغة يبقى هاي الطريقة
+
+99
+00:07:15,440 --> 00:07:19,920
+بسميها wet mouthالطريقة التانية حتى اني انا اتعرف
+
+100
+00:07:19,920 --> 00:07:22,980
+علي البكتيريا المورفولوجي اللي هي طريقة الصباغة
+
+101
+00:07:22,980 --> 00:07:26,600
+اللي احنا في هذا المعمل هنتعرف عليها خلال هذه
+
+102
+00:07:26,600 --> 00:07:30,080
+الطريقة ال organism بيكون killed stain organism
+
+103
+00:07:30,080 --> 00:07:35,420
+بيكون ميت ليش؟ لأنه من ضمن خطوات الصباغة بيتم
+
+104
+00:07:35,420 --> 00:07:40,420
+تبلير ل slide تلت مرات على ال hub هذه الخطوة بتعمل
+
+105
+00:07:40,420 --> 00:07:45,730
+قتل البكتيرياالستان لأن انا هستخدم الصبغات هستخدم
+
+106
+00:07:45,730 --> 00:07:49,550
+ال basic stain اللى بتحمل شحنة موجبة اللى بتصفغ
+
+107
+00:07:49,550 --> 00:07:55,750
+البكتيريا سالبة الشحنة in our laboratory bacterial
+
+108
+00:07:55,750 --> 00:07:59,250
+morphology form and structure may be examined in
+
+109
+00:07:59,250 --> 00:08:02,290
+two ways زى ما اتفقنا يعني دى طريقتين عشان اتعرف
+
+110
+00:08:02,290 --> 00:08:06,430
+على ال bacterial morphology by observing living
+
+111
+00:08:06,430 --> 00:08:11,250
+organism هى الطريقة بسميها ال wet mouthباستخدام
+
+112
+00:08:11,250 --> 00:08:15,830
+كيلدستان Organism اللي هنتعرف عليها في هذا المعمل
+
+113
+00:08:15,830 --> 00:08:20,630
+بما أنه صغير جدا إلى جانب أن البكتيريا حجمها صغير
+
+114
+00:08:20,630 --> 00:08:23,450
+جدا واتغلبنا على هذه المشكلة باستخدام الميكروسكوب
+
+115
+00:08:23,450 --> 00:08:27,970
+بقول لي أن البكتيريا almost are also almost
+
+116
+00:08:27,970 --> 00:08:32,210
+completely transparent شفافة colorless مايلها long
+
+117
+00:08:32,210 --> 00:08:35,450
+feet and featureless مايلها معالم in their natural
+
+118
+00:08:35,450 --> 00:08:40,780
+status عشان أحل هذه المشاكل باستخدامالستان
+
+119
+00:08:40,780 --> 00:08:44,200
+however, staining can make the structure of
+
+120
+00:08:44,200 --> 00:08:48,200
+bacteria more pronounced بقولنا إن الصبغة بتجعل
+
+121
+00:08:48,200 --> 00:08:51,820
+البكتيريا أكتر وضوحا و بقدر أني أنا أميزها
+
+122
+00:08:51,820 --> 00:08:55,780
+different type of staining method are used to make
+
+123
+00:08:55,780 --> 00:08:59,980
+the cells and their internal structure more
+
+124
+00:08:59,980 --> 00:09:04,820
+visible under the light microscopeفي عندي أنواع
+
+125
+00:09:04,820 --> 00:09:09,500
+كتيرة من الصبغات تستخدم لصباغة الخلايا والتراكيب
+
+126
+00:09:09,500 --> 00:09:14,700
+الداخلية زي الفلاجلة زي ال spark زي الكابسول وبقدر
+
+127
+00:09:14,700 --> 00:09:17,440
+أني انا اشوفها تحت ال light microscope
+
+128
+00:09:25,870 --> 00:09:29,790
+Compost أو مكونات الصبغة الصبغة من إيش بتتكون
+
+129
+00:09:29,790 --> 00:09:33,310
+بقولنا ال stain are mixture of chromogen and
+
+130
+00:09:33,310 --> 00:09:37,890
+exochrome يبقى مكونات الصبغة الصبغة بتتكون من
+
+131
+00:09:37,890 --> 00:09:42,110
+مكونات اول مكون اسمه ال chromogen والمكوين التاني
+
+132
+00:09:42,110 --> 00:09:47,150
+اسمه ال exochrome ال chromogen هو المادة الملونة
+
+133
+00:09:47,150 --> 00:09:51,230
+أما ال exochrome هو اللي بيعطي الشحنةإحنا لما
+
+134
+00:09:51,230 --> 00:09:55,310
+عرفنا الاستنان قلنا it is a substance that adhere
+
+135
+00:09:55,310 --> 00:10:00,150
+to a cell بتلتثق بالخلايا كيف بتلتثق من خلال وجود
+
+136
+00:10:00,150 --> 00:10:06,270
+تجاذب ما بين الشحنات يبقى الصبغة لازم تحمل شحنة
+
+137
+00:10:06,270 --> 00:10:09,410
+عشان يصير هذا التجاذب بينها وبين البكتيريا مين
+
+138
+00:10:09,410 --> 00:10:13,430
+اللي بيعطي الاستنان الشحنة الـ exocram الـ exocram
+
+139
+00:10:13,430 --> 00:10:17,870
+هو اللي بيحمل الشحنة نكمل التعريف giving the cell
+
+140
+00:10:17,870 --> 00:10:21,970
+color بتعطيالخلايا اللون مين بيعطي اللون أو مين
+
+141
+00:10:21,970 --> 00:10:25,550
+المسئول أو مين المادة الملونة فيه الصبغة لـ
+
+142
+00:10:25,550 --> 00:10:28,410
+Chromagen لـ Chromagen هو المادة الملونة والـ
+
+143
+00:10:28,410 --> 00:10:32,550
+Exochrome هو اللي بيعطي الشحنة بقولنا لـ Chromagen
+
+144
+00:10:32,550 --> 00:10:36,590
+بتكون من مكونات أيضًا فرعية اللي هي البنزين
+
+145
+00:10:36,590 --> 00:10:40,900
+derivative زائد لـ Chromophoreبالظبط مين من ال
+
+146
+00:10:40,900 --> 00:10:45,660
+chromogen اللي هو اللي بيعطي اللون ال chromophore
+
+147
+00:10:45,660 --> 00:10:52,180
+ال chromophore هو بالتحديد اللي بيعطي اللون للصبغة
+
+148
+00:10:52,180 --> 00:10:55,240
+هو ال coloring agent أو إذا حكينا ال chromogen
+
+149
+00:10:55,240 --> 00:10:58,660
+برضه مش غلط مين بالتحديد في ال chromogen ال
+
+150
+00:10:58,660 --> 00:11:03,940
+chromophore بالنسبة لل exochrome ال exochrome قلنا
+
+151
+00:11:03,940 --> 00:11:07,080
+اللي بيعطي الشحنة give positive or negative charge
+
+152
+00:11:07,080 --> 00:11:11,870
+to the chromogenبيعطي شحنة موجبة طبعا أو بيحمل
+
+153
+00:11:11,870 --> 00:11:16,010
+الشحنة الموجبة إذا كانت الستان هي basic stand
+
+154
+00:11:16,010 --> 00:11:20,930
+وبيعطي الشحنة السالبة إذا كانت الصبغة اللي هي
+
+155
+00:11:20,930 --> 00:11:25,910
+acidic stand the ionized stand is capable of
+
+156
+00:11:25,910 --> 00:11:29,550
+binding to cell structure with opposite charge
+
+157
+00:11:29,550 --> 00:11:33,970
+وقلنا ال ionized stand قادر على الإرتباط بتراكيب
+
+158
+00:11:33,970 --> 00:11:37,750
+الخلايا عكس شحنتها عشان يصير تجارب يبقى عنين
+
+159
+00:11:38,130 --> 00:11:43,090
+البكتيريا شحنتها سالبة المفترض تكون الصبغة بتحمل
+
+160
+00:11:43,090 --> 00:12:01,650
+شحنة عكسها يعني موجة بت الشحنة يعني basic step في
+
+161
+00:12:01,650 --> 00:12:05,350
+هال slide هنتعرف على أنواع الصبغات على حسب ال
+
+162
+00:12:05,350 --> 00:12:10,880
+chargeوقلنا إنه بتنقسم ل Basic Stain و ل Acidic
+
+163
+00:12:10,880 --> 00:12:14,840
+Stain نيجي نتعرف على ال Basic Stain و ال Acidic
+
+164
+00:12:14,840 --> 00:12:19,280
+Stain Basic Stain are cationic يعني بيحمل شحنة
+
+165
+00:12:19,280 --> 00:12:22,580
+موجبة when ionized the chromogen exhibits a
+
+166
+00:12:22,580 --> 00:12:26,580
+positive charge بيحمل شحنة موجبة بما إنه بيحمل
+
+167
+00:12:26,580 --> 00:12:31,560
+شحنة موجبة يبقى لازم يرتبط بتراكيب شحنتها سالبة
+
+168
+00:12:31,850 --> 00:12:35,250
+Basic Stain بُعيط و negatively charged cell
+
+169
+00:12:35,250 --> 00:12:39,810
+structure زي مين هذه المكونات اللي هتشحنتها سالبة؟
+
+170
+00:12:39,810 --> 00:12:44,590
+Like Nucleic Acid ذا كلها الأمثلة على أنواع من ال
+
+171
+00:12:44,590 --> 00:12:49,050
+Basic Stain مثلين بلو، crystal violet، صفرانين،
+
+172
+00:12:49,050 --> 00:12:52,310
+ملاكايت جرين، and carbofucin are common Basic
+
+173
+00:12:52,310 --> 00:12:56,790
+Stain هدول كلهم يعتبروا Basic Stain Acidic Stain
+
+174
+00:12:56,790 --> 00:13:03,120
+are anionic يعني بتحمل شحنة سالبةبما انه يحمل شحنه
+
+175
+00:13:03,120 --> 00:13:11,260
+سالبة بيحمل شحنه سالبة بيحمل شحنه سالبة بيحمل شحنه
+
+176
+00:13:11,260 --> 00:13:19,820
+سالبة بيحمل شحنه سالبة بيحمل شحنه سالبة بيحمل شحنه
+
+177
+00:13:19,820 --> 00:13:21,460
+سالبة بيحمل شحنه سالبة بيحمل شحنه سالبة بيحمل شحنه
+
+178
+00:13:21,460 --> 00:13:22,500
+سالبة بيحمل شحنه سالبة بيحمل شحنه سالبة بيحمل شحنه
+
+179
+00:13:22,500 --> 00:13:23,560
+سالبة بيحمل شحنه سالبة بيحمل شحنه سالبة بيحمل شحنه
+
+180
+00:13:23,560 --> 00:13:24,900
+سالبة بيحمل شحنه سالبة بيحمل شحنه سالبة بيحمل شحنه
+
+181
+00:13:24,900 --> 00:13:29,800
+سالبة بيحمل شحنه سالبة بBacteria are slightly
+
+182
+00:13:29,800 --> 00:13:34,120
+negatively charged at pH 7 اتفقنا ان البكتيريا
+
+183
+00:13:34,120 --> 00:13:39,820
+بتحمل شحنه صالبة في pH 7 البازيكستان مين بتصبغ
+
+184
+00:13:39,820 --> 00:13:43,520
+اتفقنا انها بتصبغ البكتيريا لان البازيكستان بتحمل
+
+185
+00:13:43,520 --> 00:13:47,360
+شحنه موجبة والبكتيريا بتحمل شحنه صالبة فهيك بيصير
+
+186
+00:13:47,360 --> 00:13:51,500
+في عندي تجاتب بين الصبغه وما بين البكتيريا او
+
+187
+00:13:51,500 --> 00:13:57,540
+الخلية أسدكستان ال acidic dye ايش بتصبغمش بتعمل
+
+188
+00:13:57,540 --> 00:14:02,960
+الـ Acidic Stain هي بتحمل شحنة سالبة يبقى بتصبغ ال
+
+189
+00:14:02,960 --> 00:14:10,860
+background اللي بتحمل شحنة موجبة هذا
+
+190
+00:14:10,860 --> 00:14:15,160
+بالنسبة للفرق ما بين ال Basic Stain و Acidic Stain
+
+191
+00:14:15,160 --> 00:14:19,360
+أو تعريفهم ممكن نعمل مقارنة من حيث تعريف كل واحدة
+
+192
+00:14:19,360 --> 00:14:23,740
+منهم شو المكونات اللي بتصبغها هاي ال Basic Stain
+
+193
+00:14:23,740 --> 00:14:34,430
+أو Acidic Stainماذا أمثلة على كل منهم؟ type
+
+194
+00:14:34,430 --> 00:14:39,650
+of staining in microbiology lab هذه أنواع الصبغات
+
+195
+00:14:39,650 --> 00:14:43,410
+اللي هنستخدمها في معمل ال microbiology لما قلنا
+
+196
+00:14:43,410 --> 00:14:46,010
+على ال basic stain و ال acidic stain هذه أنواع
+
+197
+00:14:46,010 --> 00:14:50,430
+الصبغات حسب ال charge لكن هنتكلم عن أنواع الصبغات
+
+198
+00:14:50,430 --> 00:14:54,850
+حسب ال function حسب الاستخدامفينا تلت أنواع من
+
+199
+00:14:54,850 --> 00:14:58,770
+الصبغات أول واحدة الـ Simple Stain تاني نوع الـ
+
+200
+00:14:58,770 --> 00:15:02,030
+Differential Stain تالت نوع الـ Special Stain
+
+201
+00:15:02,030 --> 00:15:05,990
+بالنسبة للـ Simple Stain اللي في هذا المعمل هنتعرف
+
+202
+00:15:05,990 --> 00:15:10,670
+عليها Simple ليش أنا سميتها Simple لأنه بتتكون فقط
+
+203
+00:15:10,670 --> 00:15:15,230
+باستخدام Reagent واحد مادة واحدة في الصباغة سهلة
+
+204
+00:15:15,230 --> 00:15:19,610
+الاستخدام وخطواتها سهلة وبسيطة من خلال الـ Simple
+
+205
+00:15:19,610 --> 00:15:23,610
+Stainبتعرف على الـ morphology شكل الخلايا الـ
+
+206
+00:15:23,610 --> 00:15:27,710
+shape و بتعرف على ال arrangement ترتيب الخلايا و
+
+207
+00:15:27,710 --> 00:15:30,850
+هنفهم بالظبط شو يعني شكل الخلايا و شو ال
+
+208
+00:15:30,850 --> 00:15:34,530
+arrangement في ال slides اللي جاية تاني نوع اللي
+
+209
+00:15:34,530 --> 00:15:37,170
+هو ال differential stain طبعا مش هحكي عنه كتير لأن
+
+210
+00:15:37,170 --> 00:15:40,430
+في معامل هنفصل فيها شو يعني differential شو يعني
+
+211
+00:15:40,430 --> 00:15:43,850
+special stain و هنتعرف على ال gram stain و ال acid
+
+212
+00:15:43,850 --> 00:15:47,410
+fast stain لكن باختصار ال differential stain من
+
+213
+00:15:47,410 --> 00:15:51,740
+اسمهاDivide bacteria into groups بتقسم البكتيريا
+
+214
+00:15:51,740 --> 00:15:56,340
+لمجموعات تميزية بتقسم البكتيريا ل group على سبيل
+
+215
+00:15:56,340 --> 00:16:00,000
+المثال جرامستان من عرفي انديانا بتقسم البكتيريا
+
+216
+00:16:00,000 --> 00:16:04,860
+لموجبة جرام و بتقسم البكتيريا لثالثة جرام لو كانت
+
+217
+00:16:04,860 --> 00:16:08,100
+البكتيريا أخدت اللون ال violet اللون ال purple
+
+218
+00:16:08,100 --> 00:16:12,540
+يبقى بعتبرها جرام positive و لو أخدت اللون ال pink
+
+219
+00:16:12,540 --> 00:16:17,610
+أو ال red بعتبرها ثالثة جرام جرام negativeأيضًا من
+
+220
+00:16:17,610 --> 00:16:20,310
+الأمثلة على الـ Differential Stain الـ Acid Fast
+
+221
+00:16:20,310 --> 00:16:23,770
+Stain برضه بتقسم للبكتيريا لـ Acid Fast بس الله يو
+
+222
+00:16:23,770 --> 00:16:27,350
+نان Acid Fast بس الله تالت نوع اللي هو الـ Special
+
+223
+00:16:27,350 --> 00:16:32,990
+Stain بتصبغ مكونات خاصة داخل الخلية البكتيرية من
+
+224
+00:16:32,990 --> 00:16:36,410
+الأمثلة على الـ Special Stain كبسولر Stain اللي هو
+
+225
+00:16:36,410 --> 00:16:41,110
+الحافظة Indospore Stain الأبوغ و الفلاجلة Stain
+
+226
+00:16:41,110 --> 00:16:45,120
+هنتعرف عليهم بالتفصيل كفة من اتين صباغتهمأحنا قلنا
+
+227
+00:16:45,120 --> 00:16:49,260
+في أول slide إن أنا بستخدم الصبغات و differentiate
+
+228
+00:16:49,260 --> 00:16:53,400
+between different organism و أحنا قلنا إن هاي
+
+229
+00:16:53,400 --> 00:16:57,360
+معناها أو بنقصد فيها differential stain way to
+
+230
+00:16:57,360 --> 00:17:00,540
+view specific part of organism و بنقصد فيها ال
+
+231
+00:17:00,540 --> 00:17:04,380
+special stain يبقى هاي استخدامات الستان بالإضافة
+
+232
+00:17:04,380 --> 00:17:09,350
+إني من خلالها بقدر أتعرف على ال morphologyزي الـ
+
+233
+00:17:09,350 --> 00:17:11,750
+Symbolistane بقدر اتعرف على الـ morphology ل
+
+234
+00:17:11,750 --> 00:17:16,030
+البكتيريا شكل البكتيريا و ال arrangement لخلايا
+
+235
+00:17:16,030 --> 00:17:20,650
+البكتيريا هذا بالنسبة لأنواع الصبغات اللي
+
+236
+00:17:20,650 --> 00:17:28,090
+بنستخدمها في معمل ال microbiology حتى
+
+237
+00:17:28,090 --> 00:17:32,390
+الجدول ذاكر اللي هم كل الصبغات الـ Symbolistane
+
+238
+00:17:32,390 --> 00:17:34,750
+الـ Differentialistane و أنواعها و ال
+
+239
+00:17:34,750 --> 00:17:38,110
+Specialistane و أنواعها لكن القدر مش مطلوب معناه
+
+240
+00:17:38,350 --> 00:17:42,350
+فقط هنتكلم على ال simple stain بعد ما نخلص كل
+
+241
+00:17:42,350 --> 00:17:45,530
+المعامل ال differential و ال special stain هذا
+
+242
+00:17:45,530 --> 00:17:50,450
+بيكون الجدول ملخص لإيه اللي هم كلهم بس هنتكلم في
+
+243
+00:17:50,450 --> 00:17:55,010
+هذا اللقاء على أول صبغة اللي هي ال simple stain
+
+244
+00:17:55,010 --> 00:17:57,630
+ذاكر جون بال simple stain يعني أنا بستخدم
+
+245
+00:17:57,630 --> 00:18:01,130
+methylene blue, carbolfaxine, crystal violet
+
+246
+00:18:01,130 --> 00:18:04,770
+وصفرانين لو متذكرين أن هدول الصبغات هم كلهم
+
+247
+00:18:04,770 --> 00:18:08,530
+يعتبروا basic stainوالـ principle لأ الـ
+
+248
+00:18:08,530 --> 00:18:11,630
+simplestin يعني أنا بصبغ ب basic stein بتحمل شحنة
+
+249
+00:18:11,630 --> 00:18:15,110
+موجبة لإن البكتيريا الشحنة الكلية لإلها شحنة سالبة
+
+250
+00:18:15,110 --> 00:18:18,770
+ليش أنا بستخدم ال simplestin used to highlight
+
+251
+00:18:18,770 --> 00:18:22,310
+microorganism to determine cellular shape and
+
+252
+00:18:22,310 --> 00:18:26,410
+arrangement بقوللي الهدف من ال simplestin يعني أنا
+
+253
+00:18:26,410 --> 00:18:29,410
+أتعرف على شكل و ترتيب الخلايا الشاب و ال
+
+254
+00:18:29,410 --> 00:18:35,130
+arrangement بستخدم في ال simplestin single basic
+
+255
+00:18:35,130 --> 00:18:40,590
+dietبتصبغ الخلايا باستخدام فقط مكون واحد او
+
+256
+00:18:40,590 --> 00:18:44,070
+reaction واحد اللي هو ال basic stain ممكن استخدم
+
+257
+00:18:44,070 --> 00:18:47,530
+مثليني blue ممكن استخدم carbon fucsin ممكن استخدم
+
+258
+00:18:47,530 --> 00:18:51,610
+crystal violet او صفرانين او ملاكايت green المهم
+
+259
+00:18:51,610 --> 00:18:55,570
+يكون basic stain ممنوع انا استخدم acidic stain
+
+260
+00:18:55,570 --> 00:18:59,030
+بقولك في بعض الأحيان ممكن تستخدمي mordant اش يعني
+
+261
+00:18:59,030 --> 00:19:02,810
+mordant موردانت يعني مثبت عشان يثبتلي اللون
+
+262
+00:19:03,160 --> 00:19:06,480
+الـMordant مثال عليه الـIodine ممكن نستخدمه ممكن
+
+263
+00:19:06,480 --> 00:19:11,020
+لأ حسب إذا هو موجود متوافر أو لأ تمام هذا بالنسبة
+
+264
+00:19:11,020 --> 00:19:14,900
+للـSimple Stain الـDifferential Stain قولنا بتميز
+
+265
+00:19:14,900 --> 00:19:18,400
+البكتيريا أو بتقسمها لمجموعات والـSpecial Stain
+
+266
+00:19:18,400 --> 00:19:22,200
+بتصبح أجزاء خاصة في الخلايا البكتيرية
+
diff --git a/PL9fwy3NUQKwZWgAb_V1ATlRRsH-6i3cPC/UNVvGjbAdO4_raw.srt b/PL9fwy3NUQKwZWgAb_V1ATlRRsH-6i3cPC/UNVvGjbAdO4_raw.srt
new file mode 100644
index 0000000000000000000000000000000000000000..8af180fd346885e209807267034f85c424dd5e75
--- /dev/null
+++ b/PL9fwy3NUQKwZWgAb_V1ATlRRsH-6i3cPC/UNVvGjbAdO4_raw.srt
@@ -0,0 +1,1064 @@
+1
+00:00:00,250 --> 00:00:04,010
+السلام عليكم ورحمة الله وبركاته نستكمل معاكم
+
+2
+00:00:04,010 --> 00:00:07,410
+محاضرات مساق الـ Microbiology العملية في هذا
+
+3
+00:00:07,410 --> 00:00:10,470
+اللقاء هتكون المحاضرة بعنوان الـ Simple Stain
+
+4
+00:00:10,470 --> 00:00:14,310
+هنتعرف على الصبغات وأنواع الصبغات اللي من ضمنها
+
+5
+00:00:14,310 --> 00:00:17,990
+الـ Simple Stain في المعمل السابق اتعرفنا أن
+
+6
+00:00:17,990 --> 00:00:22,490
+البكتيريا حجمها صغير لاترى بالعين المجردة يتراوح
+
+7
+00:00:22,490 --> 00:00:27,800
+حجمها ما بين مصر ل2 ميكرو مترعشان أتغلب على هذه
+
+8
+00:00:27,800 --> 00:00:31,020
+المشكلة استخدمت الميكروسكوب عشان يعملي
+
+9
+00:00:31,020 --> 00:00:34,940
+magnification تكبيرني أنا أقدر أشوفها من الصفات
+
+10
+00:00:34,940 --> 00:00:38,300
+الأخرى رأى البكتيريا إنها transparent شفافة
+
+11
+00:00:38,300 --> 00:00:42,440
+colorless ما إلها لون featureless ما إلها أي معالم
+
+12
+00:00:42,440 --> 00:00:46,920
+عشان أتغلب على هذه المشاكل استخدمت لستان استخدمت
+
+13
+00:00:46,920 --> 00:00:51,320
+الصبغات حتى أقدر أميزها بشكل واضح و أتعرف على
+
+14
+00:00:51,320 --> 00:00:56,600
+شكلها طيب لو بدنا نعرف لستان الصبغاتWhat is a
+
+15
+00:00:56,600 --> 00:01:00,260
+stain? A stain is a substance that adhere to a
+
+16
+00:01:00,260 --> 00:01:04,480
+cell giving the cell color هي مادة بتلتصق بالخلايا
+
+17
+00:01:04,480 --> 00:01:08,880
+و بتعطي الخلايا لون اتفقنا ان البكتيريا colorless
+
+18
+00:01:08,880 --> 00:01:13,300
+من خلال الستان هتصير color تعطيها لون وجود هذا
+
+19
+00:01:13,300 --> 00:01:16,560
+اللون the presence of color give the cell
+
+20
+00:01:16,560 --> 00:01:20,650
+significant contrast so are much more visibleوجود
+
+21
+00:01:20,650 --> 00:01:25,290
+هذا اللون هيجعل الخلايا أكثر تباينا واضوحا وسهل
+
+22
+00:01:25,290 --> 00:01:30,650
+رؤيتها و التعرف علي شكلها طيب لو احنا سألنا كيف
+
+23
+00:01:30,650 --> 00:01:35,010
+الستان كيف الصبغة بتلتسق بالخلايا بال cell او ليش
+
+24
+00:01:35,010 --> 00:01:39,870
+الصبغة بتصبغ البكتيريا وما بتصبغ أشياء في العيلة
+
+25
+00:01:39,870 --> 00:01:43,610
+بقولنا ان في عندي قوة ارتباط affinity ما بين
+
+26
+00:01:43,610 --> 00:01:48,050
+الستان وما بين ال cell طيب شو سبب قوة الارتباط هاي
+
+27
+00:01:48,960 --> 00:01:54,260
+احنا بنعرف أنه البكتيريا فيها مكونات هذه المكونات
+
+28
+00:01:54,260 --> 00:01:58,940
+في بعضها بيحمل شحنة موجبة والبعض الآخر بيحمل شحنة
+
+29
+00:01:58,940 --> 00:02:03,360
+سالبة لكن الشحنة الكلية للبكتيريا ال over all
+
+30
+00:02:03,360 --> 00:02:08,320
+charge تعتبر negative charge شحنة سالبة واحنا
+
+31
+00:02:08,320 --> 00:02:13,690
+عارفين أن الشحنات المختلفة تتجذبعشان يصير تجاذب ما
+
+32
+00:02:13,690 --> 00:02:17,670
+بين السل، ما بين الخلايا، ما بين البكتيريا وما بين
+
+33
+00:02:17,670 --> 00:02:23,650
+الصبغة للستان لازم تكون شحنة الصبغة شحنة مخالفة
+
+34
+00:02:23,650 --> 00:02:28,590
+لشحنة البكتيريا يبقى لازم تكون شحنة الصبغة شحنة
+
+35
+00:02:28,590 --> 00:02:33,590
+موجبة يعني basic stain بالتالي بيصير في عندي تجاذب
+
+36
+00:02:33,590 --> 00:02:37,900
+وارتباط وبتلتصق الصبغة على الخلايايبقى شحنة
+
+37
+00:02:37,900 --> 00:02:42,240
+البكتيريا سالبة عشان هي أنا بستخدم صبغات موجة بت
+
+38
+00:02:42,240 --> 00:02:48,060
+الشحنة صبغات basic stain طب لو عندي أنا organism
+
+39
+00:02:48,060 --> 00:02:52,060
+غير البكتيريا شحنة الكلية موجة بقى شو هستخدم
+
+40
+00:02:52,060 --> 00:02:57,660
+هستخدم صبغات سالبة الشحنة هستخدم acidic stain يبقى
+
+41
+00:02:57,660 --> 00:03:00,940
+في عندي أنا different stain اتكلمنا قولنا في عندي
+
+42
+00:03:00,940 --> 00:03:04,990
+acidic stain في عندي basic stainhave different
+
+43
+00:03:04,990 --> 00:03:08,330
+affinities for different organisms اللي هقوة
+
+44
+00:03:08,330 --> 00:03:13,230
+ارتباط مختلفة بالكائنات المختلفة عندي انا ال basic
+
+45
+00:03:13,230 --> 00:03:17,410
+stand بتصبخ كائنات بتحمل شحنة سالفة ال acidic
+
+46
+00:03:17,410 --> 00:03:21,790
+stand بتصبخ كائنات بتحمل شحنة موجة يعني يبقى قوة
+
+47
+00:03:21,790 --> 00:03:26,950
+ارتباط مختلفة لكائنات مختلفة or different part of
+
+48
+00:03:26,950 --> 00:03:31,430
+organism طيب لو انا بدي أصبغ مكون داخل الخلية
+
+49
+00:03:31,430 --> 00:03:35,820
+لسبيل مثل ال proteinوقولنا حسب هذا المكون والله
+
+50
+00:03:35,820 --> 00:03:40,600
+إذا شحنت هذا المكون صالبة بصبغ بيه شحنات موجبة
+
+51
+00:03:40,600 --> 00:03:46,420
+بصبغ بيه أعفون صبغات موجبة basic stem لو شحنت هذا
+
+52
+00:03:46,420 --> 00:03:51,700
+المكون موجبة بصبغ بيه صبغات صالبة بيه acidic stem
+
+53
+00:03:51,700 --> 00:03:57,160
+there are used to differentiate different type of
+
+54
+00:03:57,160 --> 00:04:02,090
+organism or to view specific part of organismقلنا
+
+55
+00:04:02,090 --> 00:04:06,610
+إن الستان بتعطي خلايا لون وهذا بيجعل زهر رؤيتها
+
+56
+00:04:06,610 --> 00:04:10,810
+وتمييزها ويتعرف على شكلها لكن بقول في عند
+
+57
+00:04:10,810 --> 00:04:15,630
+استخدامات أخرى لاستان استخدام آخر to differentiate
+
+58
+00:04:15,630 --> 00:04:20,010
+different type of organism عشان يفرق بين أنواع
+
+59
+00:04:20,010 --> 00:04:24,710
+مختلفة من الكائنات بسمي هذا النوع من الصبغات اللي
+
+60
+00:04:24,710 --> 00:04:29,290
+مميزلة بين أنواع مختلفة من الكائنات differential
+
+61
+00:04:30,540 --> 00:04:35,620
+تمام هنتعرف عليها بالتفصيل في معامل لاحقة هاي ال
+
+62
+00:04:35,620 --> 00:04:41,760
+differential stain بتقسم ل البكتيريا ل مجموعات or
+
+63
+00:04:41,760 --> 00:04:45,320
+to view specific part of organism او اني انا اشوف
+
+64
+00:04:45,320 --> 00:04:49,480
+مكون من مكونات ال organism وهي الصبغات بسميها
+
+65
+00:04:49,480 --> 00:04:54,460
+special stain بتصبغ مكون خاص في الخلية البكتيرية
+
+66
+00:04:54,460 --> 00:04:57,260
+على سبيل المثال بدي اصبغ ال spore ال endospore
+
+67
+00:04:57,260 --> 00:05:02,520
+stain تعتبر special stain بدي اشوفمكوّن خاص داخل
+
+68
+00:05:02,520 --> 00:05:07,960
+الخلية البكتيريا هنا قلنا ان لستان بستخدمها انا
+
+69
+00:05:07,960 --> 00:05:12,520
+عشان اتعرف على شكل البكتيريا bacterial morphology
+
+70
+00:05:12,520 --> 00:05:16,860
+يبقى لستان بشكل عام تستخدم حتى ان انا اتعرف على ال
+
+71
+00:05:16,860 --> 00:05:20,800
+bacterial morphology بقولي ال bacterial morphology
+
+72
+00:05:20,800 --> 00:05:24,680
+ممكن اتعرف عليه مش بس من خلال الصبقات في طرق تانية
+
+73
+00:05:24,680 --> 00:05:29,340
+الطريقة التانية اسمها ال wet mountهذه الطريقة
+
+74
+00:05:29,340 --> 00:05:34,380
+البكتيريا اللى أنا بشوفها بتكون living بتكون حية
+
+75
+00:05:34,380 --> 00:05:40,380
+organism غير مصبوغة كيف أنا بعمل هذه الطريقة بجيب
+
+76
+00:05:40,380 --> 00:05:44,380
+slide و بحط drop of normal saline normal saline هو
+
+77
+00:05:44,380 --> 00:05:51,360
+محلول ملحي بتركيز 85% من 100% هذا المحلول بيتم
+
+78
+00:05:51,360 --> 00:05:53,080
+تحضيره بطريقة معينة
+
+79
+00:05:55,470 --> 00:05:58,850
+وبيحافظ على الـ osmotic pressure على الضغط
+
+80
+00:05:58,850 --> 00:06:02,310
+الاصمازي بعد ما انا احط drop من ال normal saline
+
+81
+00:06:02,310 --> 00:06:05,690
+بجيب touch من البكتيريا من وين بجيب ال touch من
+
+82
+00:06:05,690 --> 00:06:09,010
+البكتيريا هذا بيكون في طبعا عندي طبق petri dish
+
+83
+00:06:09,010 --> 00:06:13,230
+بطلعه من ال incubator من الحاضنة و باخد touch من
+
+84
+00:06:13,230 --> 00:06:17,190
+البكتيريا باستخدام ال loop سواء كان بلاستيك أو بال
+
+85
+00:06:17,190 --> 00:06:22,080
+نيكروم loop و لازم يكون طبعا استيرال معقبباستخدم
+
+86
+00:06:22,080 --> 00:06:26,520
+باخد البكتيريا من طبق هذا الطبق البتري داشوي
+
+87
+00:06:26,520 --> 00:06:31,980
+باستخدام ال loop بعدها بعمل mix خفيف بين ال normal
+
+88
+00:06:31,980 --> 00:06:36,740
+saline فيه وبعد ما اخلص بحط ال cover slip و تحت ال
+
+89
+00:06:36,740 --> 00:06:41,000
+microscope بشوف بكتيريا living حية لأن انا طلعت
+
+90
+00:06:41,000 --> 00:06:45,200
+البكتيريا من ال incubator تمام understand لأنه انا
+
+91
+00:06:45,200 --> 00:06:49,340
+ما استخدمت اي صبغة خلال خطوات شغلي فبالتاني مش
+
+92
+00:06:49,340 --> 00:06:53,880
+هتكون مسبوقةنفس الفكرة لما كنتوا تعملوها في ال
+
+93
+00:06:53,880 --> 00:06:57,620
+urine analysis كنتوا تعملوها centrifugation للعينة
+
+94
+00:06:57,620 --> 00:07:00,780
+تتخلصوا من ال supernatant وتاخدوا ال sediment
+
+95
+00:07:00,780 --> 00:07:04,060
+وتحطوه على ال slide و cover slip و تحت الميكروسكوب
+
+96
+00:07:04,060 --> 00:07:08,600
+بتشوفوا البكتيريا living حية لأنك انت ماعملت اي
+
+97
+00:07:08,600 --> 00:07:12,260
+خطوة قتلة البكتيريا كانت في العينة unstained
+
+98
+00:07:12,260 --> 00:07:15,440
+ماصبغتيش مستخدمتيش اي صباغة يبقى هاي الطريقة
+
+99
+00:07:15,440 --> 00:07:19,920
+بسميها wet mouthالطريقة التانية حتى اني انا اتعرف
+
+100
+00:07:19,920 --> 00:07:22,980
+علي البكتيريا المورفولوجي اللي هي طريقة الصباغة
+
+101
+00:07:22,980 --> 00:07:26,600
+اللي احنا في هذا المعمل هنتعرف عليها خلال هذه
+
+102
+00:07:26,600 --> 00:07:30,080
+الطريقة ال organism بيكون killed stain organism
+
+103
+00:07:30,080 --> 00:07:35,420
+بيكون ميت ليش؟ لأنه من ضمن خطوات الصباغة بيتم
+
+104
+00:07:35,420 --> 00:07:40,420
+تبلير ل slide تلت مرات على ال hub هذه الخطوة بتعمل
+
+105
+00:07:40,420 --> 00:07:45,730
+قتل البكتيرياالستان لأن انا هستخدم الصبغات هستخدم
+
+106
+00:07:45,730 --> 00:07:49,550
+ال basic stain اللى بتحمل شحنة موجبة اللى بتصفغ
+
+107
+00:07:49,550 --> 00:07:55,750
+البكتيريا سالبة الشحنة in our laboratory bacterial
+
+108
+00:07:55,750 --> 00:07:59,250
+morphology form and structure may be examined in
+
+109
+00:07:59,250 --> 00:08:02,290
+two ways زى ما اتفقنا يعني دى طريقتين عشان اتعرف
+
+110
+00:08:02,290 --> 00:08:06,430
+على ال bacterial morphology by observing living
+
+111
+00:08:06,430 --> 00:08:11,250
+organism هى الطريقة بسميها ال wet mouthباستخدام
+
+112
+00:08:11,250 --> 00:08:15,830
+كيلدستان Organism اللي هنتعرف عليها في هذا المعمل
+
+113
+00:08:15,830 --> 00:08:20,630
+بما أنه صغير جدا إلى جانب أن البكتيريا حجمها صغير
+
+114
+00:08:20,630 --> 00:08:23,450
+جدا واتغلبنا على هذه المشكلة باستخدام الميكروسكوب
+
+115
+00:08:23,450 --> 00:08:27,970
+بقول لي أن البكتيريا almost are also almost
+
+116
+00:08:27,970 --> 00:08:32,210
+completely transparent شفافة colorless مايلها long
+
+117
+00:08:32,210 --> 00:08:35,450
+feet and featureless مايلها معالم in their natural
+
+118
+00:08:35,450 --> 00:08:40,780
+status عشان أحل هذه المشاكل باستخدامالستان
+
+119
+00:08:40,780 --> 00:08:44,200
+however, staining can make the structure of
+
+120
+00:08:44,200 --> 00:08:48,200
+bacteria more pronounced بقولنا إن الصبغة بتجعل
+
+121
+00:08:48,200 --> 00:08:51,820
+البكتيريا أكتر وضوحا و بقدر أني أنا أميزها
+
+122
+00:08:51,820 --> 00:08:55,780
+different type of staining method are used to make
+
+123
+00:08:55,780 --> 00:08:59,980
+the cells and their internal structure more
+
+124
+00:08:59,980 --> 00:09:04,820
+visible under the light microscopeفي عندي أنواع
+
+125
+00:09:04,820 --> 00:09:09,500
+كتيرة من الصبغات تستخدم لصباغة الخلايا والتراكيب
+
+126
+00:09:09,500 --> 00:09:14,700
+الداخلية زي الفلاجلة زي ال spark زي الكابسول وبقدر
+
+127
+00:09:14,700 --> 00:09:17,440
+أني انا اشوفها تحت ال light microscope
+
+128
+00:09:25,870 --> 00:09:29,790
+Compost أو مكونات الصبغة الصبغة من إيش بتتكون
+
+129
+00:09:29,790 --> 00:09:33,310
+بقولنا ال stain are mixture of chromogen and
+
+130
+00:09:33,310 --> 00:09:37,890
+exochrome يبقى مكونات الصبغة الصبغة بتتكون من
+
+131
+00:09:37,890 --> 00:09:42,110
+مكونات اول مكون اسمه ال chromogen والمكوين التاني
+
+132
+00:09:42,110 --> 00:09:47,150
+اسمه ال exochrome ال chromogen هو المادة الملونة
+
+133
+00:09:47,150 --> 00:09:51,230
+أما ال exochrome هو اللي بيعطي الشحنةإحنا لما
+
+134
+00:09:51,230 --> 00:09:55,310
+عرفنا الاستنان قلنا it is a substance that adhere
+
+135
+00:09:55,310 --> 00:10:00,150
+to a cell بتلتثق بالخلايا كيف بتلتثق من خلال وجود
+
+136
+00:10:00,150 --> 00:10:06,270
+تجاذب ما بين الشحنات يبقى الصبغة لازم تحمل شحنة
+
+137
+00:10:06,270 --> 00:10:09,410
+عشان يصير هذا التجاذب بينها وبين البكتيريا مين
+
+138
+00:10:09,410 --> 00:10:13,430
+اللي بيعطي الاستنان الشحنة الـ exocram الـ exocram
+
+139
+00:10:13,430 --> 00:10:17,870
+هو اللي بيحمل الشحنة نكمل التعريف giving the cell
+
+140
+00:10:17,870 --> 00:10:21,970
+color بتعطيالخلايا اللون مين بيعطي اللون أو مين
+
+141
+00:10:21,970 --> 00:10:25,550
+المسئول أو مين المادة الملونة فيه الصبغة لـ
+
+142
+00:10:25,550 --> 00:10:28,410
+Chromagen لـ Chromagen هو المادة الملونة والـ
+
+143
+00:10:28,410 --> 00:10:32,550
+Exochrome هو اللي بيعطي الشحنة بقولنا لـ Chromagen
+
+144
+00:10:32,550 --> 00:10:36,590
+بتكون من مكونات أيضًا فرعية اللي هي البنزين
+
+145
+00:10:36,590 --> 00:10:40,900
+derivative زائد لـ Chromophoreبالظبط مين من ال
+
+146
+00:10:40,900 --> 00:10:45,660
+chromogen اللي هو اللي بيعطي اللون ال chromophore
+
+147
+00:10:45,660 --> 00:10:52,180
+ال chromophore هو بالتحديد اللي بيعطي اللون للصبغة
+
+148
+00:10:52,180 --> 00:10:55,240
+هو ال coloring agent أو إذا حكينا ال chromogen
+
+149
+00:10:55,240 --> 00:10:58,660
+برضه مش غلط مين بالتحديد في ال chromogen ال
+
+150
+00:10:58,660 --> 00:11:03,940
+chromophore بالنسبة لل exochrome ال exochrome قلنا
+
+151
+00:11:03,940 --> 00:11:07,080
+اللي بيعطي الشحنة give positive or negative charge
+
+152
+00:11:07,080 --> 00:11:11,870
+to the chromogenبيعطي شحنة موجبة طبعا أو بيحمل
+
+153
+00:11:11,870 --> 00:11:16,010
+الشحنة الموجبة إذا كانت الستان هي basic stand
+
+154
+00:11:16,010 --> 00:11:20,930
+وبيعطي الشحنة السالبة إذا كانت الصبغة اللي هي
+
+155
+00:11:20,930 --> 00:11:25,910
+acidic stand the ionized stand is capable of
+
+156
+00:11:25,910 --> 00:11:29,550
+binding to cell structure with opposite charge
+
+157
+00:11:29,550 --> 00:11:33,970
+وقلنا ال ionized stand قادر على الإرتباط بتراكيب
+
+158
+00:11:33,970 --> 00:11:37,750
+الخلايا عكس شحنتها عشان يصير تجارب يبقى عنين
+
+159
+00:11:38,130 --> 00:11:43,090
+البكتيريا شحنتها سالبة المفترض تكون الصبغة بتحمل
+
+160
+00:11:43,090 --> 00:12:01,650
+شحنة عكسها يعني موجة بت الشحنة يعني basic step في
+
+161
+00:12:01,650 --> 00:12:05,350
+هال slide هنتعرف على أنواع الصبغات على حسب ال
+
+162
+00:12:05,350 --> 00:12:10,880
+chargeوقلنا إنه بتنقسم ل Basic Stain و ل Acidic
+
+163
+00:12:10,880 --> 00:12:14,840
+Stain نيجي نتعرف على ال Basic Stain و ال Acidic
+
+164
+00:12:14,840 --> 00:12:19,280
+Stain Basic Stain are cationic يعني بيحمل شحنة
+
+165
+00:12:19,280 --> 00:12:22,580
+موجبة when ionized the chromogen exhibits a
+
+166
+00:12:22,580 --> 00:12:26,580
+positive charge بيحمل شحنة موجبة بما إنه بيحمل
+
+167
+00:12:26,580 --> 00:12:31,560
+شحنة موجبة يبقى لازم يرتبط بتراكيب شحنتها سالبة
+
+168
+00:12:31,850 --> 00:12:35,250
+Basic Stain بُعيط و negatively charged cell
+
+169
+00:12:35,250 --> 00:12:39,810
+structure زي مين هذه المكونات اللي هتشحنتها سالبة؟
+
+170
+00:12:39,810 --> 00:12:44,590
+Like Nucleic Acid ذا كلها الأمثلة على أنواع من ال
+
+171
+00:12:44,590 --> 00:12:49,050
+Basic Stain مثلين بلو، crystal violet، صفرانين،
+
+172
+00:12:49,050 --> 00:12:52,310
+ملاكايت جرين، and carbofucin are common Basic
+
+173
+00:12:52,310 --> 00:12:56,790
+Stain هدول كلهم يعتبروا Basic Stain Acidic Stain
+
+174
+00:12:56,790 --> 00:13:03,120
+are anionic يعني بتحمل شحنة سالبةبما انه يحمل شحنه
+
+175
+00:13:03,120 --> 00:13:11,260
+سالبة بيحمل شحنه سالبة بيحمل شحنه سالبة بيحمل شحنه
+
+176
+00:13:11,260 --> 00:13:19,820
+سالبة بيحمل شحنه سالبة بيحمل شحنه سالبة بيحمل شحنه
+
+177
+00:13:19,820 --> 00:13:21,460
+سالبة بيحمل شحنه سالبة بيحمل شحنه سالبة بيحمل شحنه
+
+178
+00:13:21,460 --> 00:13:22,500
+سالبة بيحمل شحنه سالبة بيحمل شحنه سالبة بيحمل شحنه
+
+179
+00:13:22,500 --> 00:13:23,560
+سالبة بيحمل شحنه سالبة بيحمل شحنه سالبة بيحمل شحنه
+
+180
+00:13:23,560 --> 00:13:24,900
+سالبة بيحمل شحنه سالبة بيحمل شحنه سالبة بيحمل شحنه
+
+181
+00:13:24,900 --> 00:13:29,800
+سالبة بيحمل شحنه سالبة بBacteria are slightly
+
+182
+00:13:29,800 --> 00:13:34,120
+negatively charged at pH 7 اتفقنا ان البكتيريا
+
+183
+00:13:34,120 --> 00:13:39,820
+بتحمل شحنه صالبة في pH 7 البازيكستان مين بتصبغ
+
+184
+00:13:39,820 --> 00:13:43,520
+اتفقنا انها بتصبغ البكتيريا لان البازيكستان بتحمل
+
+185
+00:13:43,520 --> 00:13:47,360
+شحنه موجبة والبكتيريا بتحمل شحنه صالبة فهيك بيصير
+
+186
+00:13:47,360 --> 00:13:51,500
+في عندي تجاتب بين الصبغه وما بين البكتيريا او
+
+187
+00:13:51,500 --> 00:13:57,540
+الخلية أسدكستان ال acidic dye ايش بتصبغمش بتعمل
+
+188
+00:13:57,540 --> 00:14:02,960
+الـ Acidic Stain هي بتحمل شحنة سالبة يبقى بتصبغ ال
+
+189
+00:14:02,960 --> 00:14:10,860
+background اللي بتحمل شحنة موجبة هذا
+
+190
+00:14:10,860 --> 00:14:15,160
+بالنسبة للفرق ما بين ال Basic Stain و Acidic Stain
+
+191
+00:14:15,160 --> 00:14:19,360
+أو تعريفهم ممكن نعمل مقارنة من حيث تعريف كل واحدة
+
+192
+00:14:19,360 --> 00:14:23,740
+منهم شو المكونات اللي بتصبغها هاي ال Basic Stain
+
+193
+00:14:23,740 --> 00:14:34,430
+أو Acidic Stainماذا أمثلة على كل منهم؟ type
+
+194
+00:14:34,430 --> 00:14:39,650
+of staining in microbiology lab هذه أنواع الصبغات
+
+195
+00:14:39,650 --> 00:14:43,410
+اللي هنستخدمها في معمل ال microbiology لما قلنا
+
+196
+00:14:43,410 --> 00:14:46,010
+على ال basic stain و ال acidic stain هذه أنواع
+
+197
+00:14:46,010 --> 00:14:50,430
+الصبغات حسب ال charge لكن هنتكلم عن أنواع الصبغات
+
+198
+00:14:50,430 --> 00:14:54,850
+حسب ال function حسب الاستخدامفينا تلت أنواع من
+
+199
+00:14:54,850 --> 00:14:58,770
+الصبغات أول واحدة الـ Simple Stain تاني نوع الـ
+
+200
+00:14:58,770 --> 00:15:02,030
+Differential Stain تالت نوع الـ Special Stain
+
+201
+00:15:02,030 --> 00:15:05,990
+بالنسبة للـ Simple Stain اللي في هذا المعمل هنتعرف
+
+202
+00:15:05,990 --> 00:15:10,670
+عليها Simple ليش أنا سميتها Simple لأنه بتتكون فقط
+
+203
+00:15:10,670 --> 00:15:15,230
+باستخدام Reagent واحد مادة واحدة في الصباغة سهلة
+
+204
+00:15:15,230 --> 00:15:19,610
+الاستخدام وخطواتها سهلة وبسيطة من خلال الـ Simple
+
+205
+00:15:19,610 --> 00:15:23,610
+Stainبتعرف على الـ morphology شكل الخلايا الـ
+
+206
+00:15:23,610 --> 00:15:27,710
+shape و بتعرف على ال arrangement ترتيب الخلايا و
+
+207
+00:15:27,710 --> 00:15:30,850
+هنفهم بالظبط شو يعني شكل الخلايا و شو ال
+
+208
+00:15:30,850 --> 00:15:34,530
+arrangement في ال slides اللي جاية تاني نوع اللي
+
+209
+00:15:34,530 --> 00:15:37,170
+هو ال differential stain طبعا مش هحكي عنه كتير لأن
+
+210
+00:15:37,170 --> 00:15:40,430
+في معامل هنفصل فيها شو يعني differential شو يعني
+
+211
+00:15:40,430 --> 00:15:43,850
+special stain و هنتعرف على ال gram stain و ال acid
+
+212
+00:15:43,850 --> 00:15:47,410
+fast stain لكن باختصار ال differential stain من
+
+213
+00:15:47,410 --> 00:15:51,740
+اسمهاDivide bacteria into groups بتقسم البكتيريا
+
+214
+00:15:51,740 --> 00:15:56,340
+لمجموعات تميزية بتقسم البكتيريا ل group على سبيل
+
+215
+00:15:56,340 --> 00:16:00,000
+المثال جرامستان من عرفي انديانا بتقسم البكتيريا
+
+216
+00:16:00,000 --> 00:16:04,860
+لموجبة جرام و بتقسم البكتيريا لثالثة جرام لو كانت
+
+217
+00:16:04,860 --> 00:16:08,100
+البكتيريا أخدت اللون ال violet اللون ال purple
+
+218
+00:16:08,100 --> 00:16:12,540
+يبقى بعتبرها جرام positive و لو أخدت اللون ال pink
+
+219
+00:16:12,540 --> 00:16:17,610
+أو ال red بعتبرها ثالثة جرام جرام negativeأيضًا من
+
+220
+00:16:17,610 --> 00:16:20,310
+الأمثلة على الـ Differential Stain الـ Acid Fast
+
+221
+00:16:20,310 --> 00:16:23,770
+Stain برضه بتقسم للبكتيريا لـ Acid Fast بس الله يو
+
+222
+00:16:23,770 --> 00:16:27,350
+نان Acid Fast بس الله تالت نوع اللي هو الـ Special
+
+223
+00:16:27,350 --> 00:16:32,990
+Stain بتصبغ مكونات خاصة داخل الخلية البكتيرية من
+
+224
+00:16:32,990 --> 00:16:36,410
+الأمثلة على الـ Special Stain كبسولر Stain اللي هو
+
+225
+00:16:36,410 --> 00:16:41,110
+الحافظة Indospore Stain الأبوغ و الفلاجلة Stain
+
+226
+00:16:41,110 --> 00:16:45,120
+هنتعرف عليهم بالتفصيل كفة من اتين صباغتهمأحنا قلنا
+
+227
+00:16:45,120 --> 00:16:49,260
+في أول slide إن أنا بستخدم الصبغات و differentiate
+
+228
+00:16:49,260 --> 00:16:53,400
+between different organism و أحنا قلنا إن هاي
+
+229
+00:16:53,400 --> 00:16:57,360
+معناها أو بنقصد فيها differential stain way to
+
+230
+00:16:57,360 --> 00:17:00,540
+view specific part of organism و بنقصد فيها ال
+
+231
+00:17:00,540 --> 00:17:04,380
+special stain يبقى هاي استخدامات الستان بالإضافة
+
+232
+00:17:04,380 --> 00:17:09,350
+إني من خلالها بقدر أتعرف على ال morphologyزي الـ
+
+233
+00:17:09,350 --> 00:17:11,750
+Symbolistane بقدر اتعرف على الـ morphology ل
+
+234
+00:17:11,750 --> 00:17:16,030
+البكتيريا شكل البكتيريا و ال arrangement لخلايا
+
+235
+00:17:16,030 --> 00:17:20,650
+البكتيريا هذا بالنسبة لأنواع الصبغات اللي
+
+236
+00:17:20,650 --> 00:17:28,090
+بنستخدمها في معمل ال microbiology حتى
+
+237
+00:17:28,090 --> 00:17:32,390
+الجدول ذاكر اللي هم كل الصبغات الـ Symbolistane
+
+238
+00:17:32,390 --> 00:17:34,750
+الـ Differentialistane و أنواعها و ال
+
+239
+00:17:34,750 --> 00:17:38,110
+Specialistane و أنواعها لكن القدر مش مطلوب معناه
+
+240
+00:17:38,350 --> 00:17:42,350
+فقط هنتكلم على ال simple stain بعد ما نخلص كل
+
+241
+00:17:42,350 --> 00:17:45,530
+المعامل ال differential و ال special stain هذا
+
+242
+00:17:45,530 --> 00:17:50,450
+بيكون الجدول ملخص لإيه اللي هم كلهم بس هنتكلم في
+
+243
+00:17:50,450 --> 00:17:55,010
+هذا اللقاء على أول صبغة اللي هي ال simple stain
+
+244
+00:17:55,010 --> 00:17:57,630
+ذاكر جون بال simple stain يعني أنا بستخدم
+
+245
+00:17:57,630 --> 00:18:01,130
+methylene blue, carbolfaxine, crystal violet
+
+246
+00:18:01,130 --> 00:18:04,770
+وصفرانين لو متذكرين أن هدول الصبغات هم كلهم
+
+247
+00:18:04,770 --> 00:18:08,530
+يعتبروا basic stainوالـ principle لأ الـ
+
+248
+00:18:08,530 --> 00:18:11,630
+simplestin يعني أنا بصبغ ب basic stein بتحمل شحنة
+
+249
+00:18:11,630 --> 00:18:15,110
+موجبة لإن البكتيريا الشحنة الكلية لإلها شحنة سالبة
+
+250
+00:18:15,110 --> 00:18:18,770
+ليش أنا بستخدم ال simplestin used to highlight
+
+251
+00:18:18,770 --> 00:18:22,310
+microorganism to determine cellular shape and
+
+252
+00:18:22,310 --> 00:18:26,410
+arrangement بقوللي الهدف من ال simplestin يعني أنا
+
+253
+00:18:26,410 --> 00:18:29,410
+أتعرف على شكل و ترتيب الخلايا الشاب و ال
+
+254
+00:18:29,410 --> 00:18:35,130
+arrangement بستخدم في ال simplestin single basic
+
+255
+00:18:35,130 --> 00:18:40,590
+dietبتصبغ الخلايا باستخدام فقط مكون واحد او
+
+256
+00:18:40,590 --> 00:18:44,070
+reaction واحد اللي هو ال basic stain ممكن استخدم
+
+257
+00:18:44,070 --> 00:18:47,530
+مثليني blue ممكن استخدم carbon fucsin ممكن استخدم
+
+258
+00:18:47,530 --> 00:18:51,610
+crystal violet او صفرانين او ملاكايت green المهم
+
+259
+00:18:51,610 --> 00:18:55,570
+يكون basic stain ممنوع انا استخدم acidic stain
+
+260
+00:18:55,570 --> 00:18:59,030
+بقولك في بعض الأحيان ممكن تستخدمي mordant اش يعني
+
+261
+00:18:59,030 --> 00:19:02,810
+mordant موردانت يعني مثبت عشان يثبتلي اللون
+
+262
+00:19:03,160 --> 00:19:06,480
+الـMordant مثال عليه الـIodine ممكن نستخدمه ممكن
+
+263
+00:19:06,480 --> 00:19:11,020
+لأ حسب إذا هو موجود متوافر أو لأ تمام هذا بالنسبة
+
+264
+00:19:11,020 --> 00:19:14,900
+للـSimple Stain الـDifferential Stain قولنا بتميز
+
+265
+00:19:14,900 --> 00:19:18,400
+البكتيريا أو بتقسمها لمجموعات والـSpecial Stain
+
+266
+00:19:18,400 --> 00:19:22,200
+بتصبح أجزاء خاصة في الخلايا البكتيرية
+
diff --git a/PL9fwy3NUQKwZWgAb_V1ATlRRsH-6i3cPC/Ul-B8e_odMk_raw.json b/PL9fwy3NUQKwZWgAb_V1ATlRRsH-6i3cPC/Ul-B8e_odMk_raw.json
new file mode 100644
index 0000000000000000000000000000000000000000..5d057695f388c1eae0d0c275a6aafd52b3372049
--- /dev/null
+++ b/PL9fwy3NUQKwZWgAb_V1ATlRRsH-6i3cPC/Ul-B8e_odMk_raw.json
@@ -0,0 +1 @@
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a و b بتضيفي reagent c اللي هو ال zinc powder ال zinc powder يعتبر reducing agent طبعا هضيف reagent c بقوللي بعد إضافة reagent c في حال كانت النتيجة colorless بعد إضافة reagent a و b إذا اللون اتحول للون red معناه هي النتيجة negative", "tokens": [41361, 13672, 11536, 2017, 1832, 19446, 1975, 2655, 17082, 13546, 39182, 11933, 11242, 31845, 3660, 26949, 317, 257, 4032, 272, 39894, 11242, 33911, 1829, 26949, 317, 269, 13672, 1829, 31439, 2423, 29062, 6341, 2423, 29062, 6341, 7251, 34268, 26890, 12245, 9461, 23032, 3555, 3615, 995, 8032, 11242, 33911, 26949, 317, 269, 4724, 39648, 20292, 39182, 11933, 11242, 31845, 3660, 26949, 317, 269, 8978, 11331, 6027, 25961, 2655, 28239, 31371, 7435, 3660, 2017, 1832, 39182, 11933, 11242, 31845, 3660, 26949, 317, 257, 4032, 272, 11933, 15730, 13672, 11536, 1975, 2655, 5016, 12610, 5296, 1211, 11536, 2182, 20449, 8315, 3224, 39896, 28239, 31371, 7435, 3660, 3671], "avg_logprob": -0.14523808842613584, "compression_ratio": 1.9375, "no_speech_prob": 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procedure و أمثلة على البكتيريا اللى بتتحول فقط الـ nitrate ل nitrite أمثلة على البكتيريا اللى بتتحول ال nitrate ل molecular nitrogen بتعمل complete reduction الصدمه و نصب البكتيريا اللى ما عندها أنزيم للـ Staphylococcus epidermidis", "tokens": [3224, 15730, 20666, 1863, 35457, 3660, 24976, 39184, 10747, 4032, 5551, 2304, 12984, 37977, 15844, 29739, 4117, 2655, 13546, 25528, 13672, 7578, 39894, 2655, 5016, 12610, 6156, 47432, 2423, 39184, 10900, 4404, 5296, 10900, 35002, 5551, 2304, 12984, 37977, 15844, 29739, 4117, 2655, 13546, 25528, 13672, 7578, 39894, 2655, 5016, 12610, 2423, 10900, 4404, 5296, 19046, 17903, 39894, 25957, 1211, 3566, 11004, 31767, 40448, 3224, 4032, 8717, 9381, 3555, 29739, 4117, 2655, 13546, 25528, 13672, 7578, 19446, 43242, 11296, 14739, 11622, 32640, 24976, 39184, 745, 569, 3495, 5842, 905, 1149, 13510, 966, 40920], "avg_logprob": -0.20096409574468085, "compression_ratio": 1.7740384615384615, "no_speech_prob": 5.960464477539063e-08, "words": [{"start": 917.91, 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البكتيريا أو الإنزيم الموجود في البكتيريا", "tokens": [3555, 22488, 11933, 11242, 31845, 3660, 1300, 559, 3020, 11933, 15730, 1975, 2655, 5016, 12610, 13672, 11536, 5296, 1211, 11536, 5551, 5016, 29973, 23758, 20449, 8315, 3224, 36145, 3224, 8717, 31371, 7435, 3660, 3671, 13672, 11536, 16247, 5016, 29973, 8717, 2655, 7435, 8717, 31371, 7435, 3660, 36145, 3224, 46811, 1975, 46456, 11622, 6027, 2423, 37942, 4404, 5296, 37942, 35002, 4724, 995, 14851, 9778, 3215, 10943, 2423, 1176, 4647, 35781, 4032, 20292, 3794, 4724, 35457, 3555, 29739, 4117, 2655, 13546, 25528, 34051, 33688, 1863, 11622, 32640, 9673, 29245, 23328, 8978, 29739, 4117, 2655, 13546, 25528], "avg_logprob": -0.16324013377490795, "compression_ratio": 1.661764705882353, "no_speech_prob": 2.6524066925048828e-05, "words": [{"start": 957.5, "end": 957.84, "word": "بعد", "probability": 0.929931640625}, {"start": 957.84, "end": 958.3, "word": " إضافة", "probability": 0.97314453125}, {"start": 958.3, "end": 958.92, "word": " 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reagent B dimethyl naphthalamide هضيفهم على المزرعة بعد ال incubation 24 ساعة بقوللي any nitrite in the media أي نيتريت موجود في ال media هيتفاعل مع هذه ال reagent وهيعطيني red color يبقى", "tokens": [32277, 2304, 3224, 1845, 950, 282, 388, 299, 8258, 26949, 317, 4724, 32277, 2304, 3224, 2423, 39184, 5013, 3293, 5088, 9296, 49123, 335, 482, 7251, 3555, 4587, 7578, 11296, 26949, 317, 316, 1845, 950, 282, 388, 299, 8258, 26949, 317, 363, 5013, 3293, 5088, 9296, 49123, 335, 482, 8032, 11242, 33911, 16095, 15844, 9673, 11622, 2288, 27884, 39182, 2423, 33345, 399, 4022, 8608, 995, 27884, 4724, 39648, 20292, 604, 10900, 35002, 294, 264, 3021, 36632, 8717, 36081, 2288, 36081, 3714, 29245, 23328, 8978, 2423, 3021, 39896, 2655, 5172, 995, 30241, 20449, 29538, 2423, 26949, 317, 37037, 1829, 3615, 9566, 1829, 22653, 2182, 2017, 7251, 3555, 4587, 7578], "avg_logprob": -0.31076388585346715, "compression_ratio": 1.6, "no_speech_prob": 1.0967254638671875e-05, "words": [{"start": 1038.52, "end": 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129389, "start": 1268.61, "end": 1293.89, "text": "كيف ال procedure قلنا بستخدم نيترات ال breath فيها بوتاسيوم نيترات نصف المية بعمل incubation هضيف خمسة drop من reactant A وخمسة drop من reactant B بقولي if nitrate is present in the media it will turn red within one to two minutes يعني ان في غلط في ال slide نغيرها ل nitrite", "tokens": [4117, 33911, 2423, 10747, 12174, 1211, 8315, 4724, 14851, 9778, 40448, 8717, 36081, 2288, 9307, 2423, 6045, 8978, 11296, 4724, 35473, 32277, 1829, 20498, 8717, 36081, 2288, 9307, 8717, 9381, 5172, 9673, 10632, 4724, 25957, 1211, 33345, 399, 8032, 11242, 33911, 16490, 2304, 3794, 3660, 3270, 9154, 4515, 394, 316, 4032, 9778, 2304, 3794, 3660, 3270, 9154, 4515, 394, 363, 4724, 39648, 1829, 498, 10900, 4404, 307, 1974, 294, 264, 3021, 309, 486, 1261, 2182, 1951, 472, 281, 732, 2077, 37495, 22653, 16472, 8978, 32771, 1211, 9566, 8978, 2423, 4137, 8717, 17082, 13546, 11296, 5296, 10900, 35002], "avg_logprob": -0.24569514834759187, "compression_ratio": 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نفط القمعين يعتبر carcinogenic عشان هيك", "tokens": [3224, 8315, 4724, 39648, 1829, 11778, 1829, 8978, 45030, 11242, 2423, 4514, 4474, 43242, 44713, 9778, 3215, 10943, 26949, 317, 13672, 1829, 31439, 6156, 5016, 9381, 2423, 22653, 2655, 2288, 9307, 5480, 1500, 12174, 12610, 1829, 26949, 317, 4724, 1829, 3224, 13672, 1829, 31439, 2423, 6339, 50113, 1211, 8717, 5172, 9566, 25062, 2304, 3615, 9957, 7251, 34268, 26890, 1032, 20021, 25473, 6225, 8592, 7649, 39896, 4117], "avg_logprob": -0.27402051793995186, "compression_ratio": 1.4508670520231215, "no_speech_prob": 2.6226043701171875e-06, "words": [{"start": 1933.62, "end": 1933.9, "word": "هنا", "probability": 0.585693359375}, {"start": 1933.9, "end": 1934.22, "word": " بقولي", "probability": 0.7816569010416666}, {"start": 1934.22, "end": 1934.52, "word": " دي", "probability": 0.705078125}, {"start": 1934.52, "end": 1935.14, "word": " في", "probability": 0.92138671875}, {"start": 1935.14, "end": 1935.52, "word": " بعض", "probability": 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"probability": 0.90380859375}, {"start": 1940.76, "end": 1941.68, "word": " قولي", "probability": 0.7527669270833334}, {"start": 1941.68, "end": 1942.94, "word": " reagent", "probability": 0.931640625}, {"start": 1942.94, "end": 1943.3, "word": " بيه", "probability": 0.7874348958333334}, {"start": 1943.3, "end": 1943.44, "word": " اللي", "probability": 0.955322265625}, {"start": 1943.44, "end": 1943.74, "word": " هو", "probability": 0.94873046875}, {"start": 1943.74, "end": 1943.94, "word": " ال", "probability": 0.830078125}, {"start": 1943.94, "end": 1944.14, "word": " diet", "probability": 0.059539794921875}, {"start": 1944.14, "end": 1944.54, "word": " مثل", "probability": 0.90185546875}, {"start": 1944.54, "end": 1944.88, "word": " نفط", "probability": 0.9554036458333334}, {"start": 1944.88, "end": 1945.4, "word": " القمعين", "probability": 0.7698974609375}, {"start": 1945.4, "end": 1945.76, "word": " يعتبر", "probability": 0.9111328125}, {"start": 1945.76, "end": 1946.64, "word": 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"probability": 0.97802734375}, {"start": 1951.97, "end": 1952.95, "word": " جلدك", "probability": 0.8524169921875}, {"start": 1952.95, "end": 1953.17, "word": " هذه", "probability": 0.47802734375}, {"start": 1953.17, "end": 1953.81, "word": " المواد", "probability": 0.9344075520833334}, {"start": 1953.81, "end": 1954.31, "word": " و", "probability": 0.958984375}, {"start": 1954.31, "end": 1954.75, "word": " كمان", "probability": 0.7919108072916666}, {"start": 1954.75, "end": 1955.85, "word": " انك", "probability": 0.7431640625}, {"start": 1955.85, "end": 1956.41, "word": " تستنشق", "probability": 0.96640625}, {"start": 1956.41, "end": 1956.65, "word": " هذه", "probability": 0.77587890625}, {"start": 1956.65, "end": 1957.23, "word": " المواد", "probability": 0.9674479166666666}, {"start": 1957.23, "end": 1958.13, "word": " كمان", "probability": 0.9109700520833334}, {"start": 1958.13, "end": 1958.37, "word": " ال", "probability": 0.266845703125}, {"start": 1958.37, "end": 1958.81, "word": " reagent", "probability": 0.822998046875}, {"start": 1958.81, "end": 1959.07, "word": " is", "probability": 0.1826171875}, {"start": 1959.07, "end": 1959.73, "word": " sulfonylic", "probability": 0.6560872395833334}, {"start": 1959.73, "end": 1960.03, "word": " acid", "probability": 0.943359375}, {"start": 1960.03, "end": 1960.47, "word": " يعتبر", "probability": 0.9446614583333334}, {"start": 1960.47, "end": 1961.17, "word": " كوزاتك", "probability": 0.73896484375}, {"start": 1961.17, "end": 1961.63, "word": " مواد", "probability": 0.9099934895833334}, {"start": 1961.63, "end": 1962.27, "word": " حارقة", "probability": 0.9798177083333334}, {"start": 1962.27, "end": 1964.65, "word": " طبعا", "probability": 0.976806640625}, {"start": 1964.65, "end": 1965.07, "word": " avoid", "probability": 0.8271484375}, {"start": 1965.07, "end": 1965.67, "word": " skin", "probability": 0.9384765625}, {"start": 1965.67, "end": 1966.39, "word": " contact", "probability": 0.95263671875}, {"start": 1966.39, "end": 1966.99, "word": " بحاول", "probability": 0.967041015625}, {"start": 1966.99, "end": 1967.21, "word": " أني", "probability": 0.7236328125}, {"start": 1967.21, "end": 1967.65, "word": " أبعده", "probability": 0.8765869140625}, {"start": 1967.65, "end": 1967.93, "word": " عن", "probability": 0.990234375}, {"start": 1967.93, "end": 1968.57, "word": " الجلد", "probability": 0.92822265625}, {"start": 1968.57, "end": 1968.67, "word": " و", "probability": 0.99169921875}, {"start": 1968.67, "end": 1968.79, "word": " عن", "probability": 0.93212890625}, {"start": 1968.79, "end": 1969.11, "word": " إيدي", "probability": 0.8486328125}, {"start": 1969.11, "end": 1969.27, "word": " و", "probability": 0.9892578125}, {"start": 1969.27, "end": 1969.45, "word": " ما", "probability": 0.5966796875}, {"start": 1969.45, "end": 1969.83, "word": " أني", "probability": 0.78271484375}, {"start": 1969.83, "end": 1969.97, "word": " أنا", "probability": 0.46484375}, {"start": 1969.97, "end": 1970.73, "word": " أستنشقه", "probability": 0.9711100260416666}], "temperature": 1.0}, {"id": 79, "seek": 197877, "start": 1971.07, "end": 1978.77, "text": "كمان أيضا عند استخدام الزنك باودر بيقوللي ممنوع انك تستنشقيها او انه تلمس جلدك", "tokens": [24793, 7649, 36632, 11242, 995, 43242, 44713, 9778, 3215, 10943, 6024, 110, 1863, 4117, 4724, 995, 23328, 2288, 4724, 1829, 39648, 20292, 3714, 27842, 45367, 16472, 4117, 6055, 14851, 1863, 8592, 38436, 11296, 1975, 2407, 16472, 3224, 6055, 19528, 3794, 10874, 1211, 3215, 4117], "avg_logprob": -0.18229166401757135, "compression_ratio": 1.375, "no_speech_prob": 5.960464477539063e-08, "words": [{"start": 1971.07, "end": 1971.65, "word": "كمان", "probability": 0.620849609375}, {"start": 1971.65, "end": 1972.21, "word": " أيضا", "probability": 0.8356119791666666}, {"start": 1972.21, "end": 1972.83, "word": " عند", "probability": 0.95263671875}, {"start": 1972.83, "end": 1973.39, "word": " استخدام", "probability": 0.978271484375}, {"start": 1973.39, "end": 1973.81, "word": " الزنك", "probability": 0.769775390625}, {"start": 1973.81, "end": 1974.29, "word": " باودر", "probability": 0.8907470703125}, {"start": 1974.29, "end": 1974.81, "word": " بيقوللي", "probability": 0.702117919921875}, {"start": 1974.81, "end": 1975.95, "word": " ممنوع", "probability": 0.97607421875}, {"start": 1975.95, "end": 1976.15, "word": " انك", "probability": 0.801025390625}, {"start": 1976.15, "end": 1977.01, "word": " تستنشقيها", "probability": 0.981689453125}, {"start": 1977.01, "end": 1977.31, "word": " او", "probability": 0.846923828125}, {"start": 1977.31, "end": 1977.71, "word": " انه", "probability": 0.847900390625}, {"start": 1977.71, "end": 1978.27, "word": " تلمس", "probability": 0.9806315104166666}, {"start": 1978.27, "end": 1978.77, "word": " جلدك", "probability": 0.97705078125}], "temperature": 1.0}, {"id": 80, "seek": 200221, "start": 1979.01, "end": 2002.21, "text": "ممنوع تستخدمي الـ mouth by beating في فحص الـ Nitrate Reduction Test لأن كل ال reagent اللي بنستخدمها تعتبر طبعا خطيرة، هيك من جون خلصنا الفحص ال Nitrate Reduction Test وخلصنا شعب ترب التلات فحوصات ال Ureas و ال Endol و ال Nitrate Reduction Test السلام عليكم و رحمة الله وبركاته", "tokens": [2304, 27842, 45367, 6055, 14851, 9778, 40448, 1829, 2423, 39184, 4525, 538, 13497, 8978, 6156, 5016, 9381, 2423, 39184, 37942, 4404, 4477, 27549, 9279, 5296, 33456, 28242, 2423, 26949, 317, 13672, 1829, 44945, 14851, 9778, 40448, 11296, 6055, 34268, 26890, 23032, 3555, 3615, 995, 16490, 9566, 48923, 12399, 39896, 4117, 9154, 10874, 11536, 16490, 1211, 9381, 8315, 27188, 5016, 9381, 2423, 37942, 4404, 4477, 27549, 9279, 4032, 9778, 1211, 9381, 8315, 13412, 3615, 3555, 6055, 25513, 16712, 1211, 9307, 6156, 5016, 2407, 9381, 9307, 2423, 624, 37868, 4032, 2423, 6967, 401, 4032, 2423, 37942, 4404, 4477, 27549, 9279, 21136, 37440, 25894, 24793, 4032, 12602, 35571, 3660, 21984, 4032, 26890, 4117, 9307, 3224], "avg_logprob": -0.24695796038197204, "compression_ratio": 1.7148760330578512, "no_speech_prob": 3.129243850708008e-05, "words": [{"start": 1979.01, "end": 1979.39, "word": "ممنوع", "probability": 0.8606770833333334}, {"start": 1979.39, "end": 1980.11, "word": " تستخدمي", "probability": 0.90986328125}, {"start": 1980.11, "end": 1980.41, "word": " الـ", "probability": 0.5318603515625}, {"start": 1980.41, "end": 1980.63, "word": " mouth", "probability": 0.461181640625}, {"start": 1980.63, "end": 1980.89, "word": " by", "probability": 0.5419921875}, {"start": 1980.89, "end": 1981.27, "word": " beating", "probability": 0.09210205078125}, {"start": 1981.27, "end": 1981.45, "word": " في", "probability": 0.8173828125}, {"start": 1981.45, "end": 1981.85, "word": " فحص", "probability": 0.9794921875}, {"start": 1981.85, "end": 1982.05, "word": " الـ", "probability": 0.78515625}, {"start": 1982.05, "end": 1982.37, "word": " Nitrate", "probability": 0.7032470703125}, {"start": 1982.37, "end": 1982.91, "word": " Reduction", "probability": 0.7490234375}, {"start": 1982.91, "end": 1983.21, "word": " Test", "probability": 0.833984375}, {"start": 1983.21, "end": 1983.49, "word": " لأن", "probability": 0.873046875}, {"start": 1983.49, "end": 1983.77, "word": " كل", "probability": 0.8916015625}, {"start": 1983.77, "end": 1983.87, "word": " ال", "probability": 0.94921875}, {"start": 1983.87, "end": 1984.23, "word": " reagent", "probability": 0.8525390625}, {"start": 1984.23, "end": 1984.37, "word": " اللي", "probability": 0.836181640625}, {"start": 1984.37, "end": 1985.01, "word": " بنستخدمها", "probability": 0.95537109375}, {"start": 1985.01, "end": 1985.55, "word": " تعتبر", "probability": 0.9039713541666666}, {"start": 1985.55, "end": 1987.03, "word": " طبعا", "probability": 0.84576416015625}, {"start": 1987.03, "end": 1988.21, "word": " خطيرة،", "probability": 0.7447509765625}, {"start": 1988.21, "end": 1988.45, "word": " هيك", "probability": 0.73291015625}, {"start": 1988.45, "end": 1988.59, "word": " من", "probability": 0.931640625}, {"start": 1988.59, "end": 1988.71, "word": " جون", "probability": 0.6409912109375}, {"start": 1988.71, "end": 1989.43, "word": " خلصنا", "probability": 0.988525390625}, {"start": 1989.43, "end": 1990.39, "word": " الفحص", "probability": 0.8543294270833334}, {"start": 1990.39, "end": 1990.51, "word": " ال", "probability": 0.7841796875}, {"start": 1990.51, "end": 1991.03, "word": " Nitrate", "probability": 0.80126953125}, {"start": 1991.03, "end": 1991.59, "word": " Reduction", "probability": 0.967529296875}, {"start": 1991.59, "end": 1991.99, "word": " Test", "probability": 0.9091796875}, {"start": 1991.99, "end": 1992.79, "word": " وخلصنا", "probability": 0.89375}, {"start": 1992.79, "end": 1993.49, "word": " شعب", "probability": 0.621826171875}, {"start": 1993.49, "end": 1993.81, "word": " ترب", "probability": 0.4837646484375}, {"start": 1993.81, "end": 1995.39, "word": " التلات", "probability": 0.9207356770833334}, {"start": 1995.39, "end": 1995.93, "word": " فحوصات", "probability": 0.8080078125}, {"start": 1995.93, "end": 1996.09, "word": " ال", "probability": 0.88916015625}, {"start": 1996.09, "end": 1996.57, "word": " Ureas", "probability": 0.40899658203125}, {"start": 1996.57, "end": 1996.71, "word": " و", "probability": 0.72509765625}, {"start": 1996.71, "end": 1996.81, "word": " ال", "probability": 0.52490234375}, {"start": 1996.81, "end": 1997.35, "word": " Endol", "probability": 0.60546875}, {"start": 1997.35, "end": 1997.93, "word": " و", "probability": 0.9423828125}, {"start": 1997.93, "end": 1998.25, "word": " ال", "probability": 0.7744140625}, {"start": 1998.25, "end": 1998.83, "word": " Nitrate", "probability": 0.964599609375}, {"start": 1998.83, "end": 1999.33, "word": " Reduction", "probability": 0.96435546875}, {"start": 1999.33, "end": 1999.65, "word": " Test", "probability": 0.921875}, {"start": 1999.65, "end": 2000.05, "word": " السلام", "probability": 0.841064453125}, {"start": 2000.05, "end": 2000.63, "word": " عليكم", "probability": 0.940185546875}, {"start": 2000.63, "end": 2000.81, "word": " و", "probability": 0.998046875}, {"start": 2000.81, "end": 2001.11, "word": " رحمة", "probability": 0.9060872395833334}, {"start": 2001.11, "end": 2001.41, "word": " الله", "probability": 0.97314453125}, {"start": 2001.41, "end": 2002.21, "word": " وبركاته", "probability": 0.93564453125}], "temperature": 1.0}], "language": "ar", "language_probability": 1.0, "duration": 2003.882125, "duration_after_vad": 1885.8006249999937}
\ No newline at end of file
diff --git a/PL9fwy3NUQKwZWgAb_V1ATlRRsH-6i3cPC/Ul-B8e_odMk_raw.srt b/PL9fwy3NUQKwZWgAb_V1ATlRRsH-6i3cPC/Ul-B8e_odMk_raw.srt
new file mode 100644
index 0000000000000000000000000000000000000000..e9ce96b740a69f542dcb575aa369b42b0160a2e2
--- /dev/null
+++ b/PL9fwy3NUQKwZWgAb_V1ATlRRsH-6i3cPC/Ul-B8e_odMk_raw.srt
@@ -0,0 +1,1792 @@
+1
+00:00:02,210 --> 00:00:06,090
+Nitrate reduction test Nitrate reduction test is a
+
+2
+00:00:06,090 --> 00:00:09,010
+test to differentiate between bacteria based on
+
+3
+00:00:09,010 --> 00:00:14,030
+their ability or inability to reduce nitrate NO3
+
+4
+00:00:14,030 --> 00:00:20,030
+to nitrite NO2 using anaerobic respiration بقوللي
+
+5
+00:00:20,030 --> 00:00:24,630
+هذا الفحص أنا بستخدمه للتفريق بين البكتيريا وهل
+
+6
+00:00:24,630 --> 00:00:29,030
+عندها القدرة او ما عندها القدرة انها تختزل to
+
+7
+00:00:29,030 --> 00:00:34,360
+reduce nitrateللنيترات بدي اكشفها للبكتيريا عندها
+
+8
+00:00:34,360 --> 00:00:40,260
+القدرة انها تختزل النيترات لنيترات يبقى النيترات
+
+9
+00:00:40,260 --> 00:00:46,040
+هي ال substrate اللى هى يشتغل عليها الانزيم هيتم
+
+10
+00:00:46,040 --> 00:00:50,800
+الاختزال بواسطة انزيم نيترات ريدكتاز من اسمه
+
+11
+00:00:50,800 --> 00:00:54,940
+نيترات ريدكتاز يبقى هيعمل اختزال للنيترات لل
+
+12
+00:00:54,940 --> 00:01:00,630
+substrate هيتم اختزالها لنيتراتهيتم استخدام الـ
+
+13
+00:01:00,630 --> 00:01:04,810
+nitrate في الـ anaerobic respiration احنا قلنا في
+
+14
+00:01:04,810 --> 00:01:08,890
+ال anaerobic respiration بستخدم inorganic molecule
+
+15
+00:01:08,890 --> 00:01:13,090
+كمستقبل لنهائي للإلكترونات من ضمن ال inorganic
+
+16
+00:01:13,090 --> 00:01:17,950
+molecule كان ال nitrate فبالتالي بيتم استخدامه في
+
+17
+00:01:17,950 --> 00:01:22,450
+ال anaerobic respiration يبقى في بعض البكتيريا
+
+18
+00:01:22,450 --> 00:01:25,690
+عندها أنزيم ال nitrate reductase بتعمل اختزال
+
+19
+00:01:25,690 --> 00:01:31,340
+لنيترات لنيتراتsome of these bacteria بقولي بعض
+
+20
+00:01:31,340 --> 00:01:34,980
+البكتيريا اللي عندها أنزيم النيترات reductase both
+
+21
+00:01:34,980 --> 00:01:38,680
+of the enzyme the two either reduce the nitrite to
+
+22
+00:01:38,680 --> 00:01:41,660
+either the ammonium or molecular nitrogen
+
+23
+00:01:41,660 --> 00:01:45,260
+البكتيريا اللي عندها أنزيم النيترات reductase بعد
+
+24
+00:01:45,260 --> 00:01:50,140
+ما تختزل النيترات لنيترات بقولي ممكن يتم اختزال
+
+25
+00:01:50,140 --> 00:01:54,760
+النيترات ل ammonium ion يبقى بعد ما يتم اختزال
+
+26
+00:01:54,760 --> 00:02:00,640
+النيترات البكتيريا عشان تستفيد منهممكن إنها تحصل
+
+27
+00:02:00,640 --> 00:02:03,940
+على نيتروجين source فبالتالي بتعمل اختزال
+
+28
+00:02:03,940 --> 00:02:09,260
+للنيترائت ل ammonium ion و بقول لي ممكن يتم اختزال
+
+29
+00:02:09,260 --> 00:02:13,280
+النيترائت ل molecular nitrogen كيف هيتم اختزال
+
+30
+00:02:13,280 --> 00:02:17,560
+النيترائت ل molecular nitrogen من خلال هذا
+
+31
+00:02:17,560 --> 00:02:23,810
+التفاعلات اللي هتصير زي ما هي موضحة في الصورةبقولي
+
+32
+00:02:23,810 --> 00:02:26,890
+بعد ما يتم اختزال الـ Nitrate لـ Nitrite بواسط
+
+33
+00:02:26,890 --> 00:02:30,730
+انزيم الـ Nitrate Reductase بيتم اختزال الـ
+
+34
+00:02:30,730 --> 00:02:34,630
+Nitrite لـ Nitric Oxide باستخدام انزيم الـ Nitrate
+
+35
+00:02:34,630 --> 00:02:39,710
+Reductase لأنه هيتم اختزال الـ Nitrite لـ Nitric
+
+36
+00:02:39,710 --> 00:02:43,830
+Oxide فبالتالي اسم الانزيم Nitrate Reductase بعد
+
+37
+00:02:43,830 --> 00:02:48,170
+ما يتم اختزال الـ Nitrite لـ Nitric Oxide الـ
+
+38
+00:02:48,170 --> 00:02:51,890
+Nitric Oxide برضه هيتم اختزاله لـ Nitrous Oxide اش
+
+39
+00:02:51,890 --> 00:02:57,070
+اسم الانزيم؟بنضيف Redactase هيعمل اختزال ل ال
+
+40
+00:02:57,070 --> 00:03:00,490
+substrate اللى قبل هيبقى اسمه Nitric Oxide
+
+41
+00:03:00,490 --> 00:03:05,090
+Redactase برضه ال Nitrous Oxide بعد ما يتم اختزاله
+
+42
+00:03:05,090 --> 00:03:08,610
+هيتم اختزاله لنيتروجين باستخدام أنزيم ال Nitrous
+
+43
+00:03:08,610 --> 00:03:11,770
+Oxide Redactase لأنه شتغل على ال Nitrous Oxide
+
+44
+00:03:11,770 --> 00:03:16,030
+فبالتالي بسميه Nitrous Oxide Redactase بالنهاية
+
+45
+00:03:16,030 --> 00:03:20,810
+هحصل على نيتروجين على molecular nitrogenهك عندي
+
+46
+00:03:20,810 --> 00:03:24,350
+أنا مصدر للـ nitrogen بتقدر البكتيريا تنمو وتتكثر
+
+47
+00:03:24,350 --> 00:03:29,250
+يبقى في بعض البكتيريا عندها أنزيم الـ nitrate
+
+48
+00:03:29,250 --> 00:03:34,710
+reductase هتعمل اختزال للنيتريات لـ nitrite في بعض
+
+49
+00:03:34,710 --> 00:03:39,150
+البكتيريا اللي عندها هذا الانزيم هيتم اختزال الـ
+
+50
+00:03:39,150 --> 00:03:43,950
+nitrite لـ ammonium ion أو ممكن يصير عندي complete
+
+51
+00:03:43,950 --> 00:03:48,330
+reduction زي ما هو موضح في الصورة في التفاعلات
+
+52
+00:03:48,330 --> 00:03:53,180
+التاليةبالنهاية هحصل على molecular nitrogen يبقى
+
+53
+00:03:53,180 --> 00:03:56,180
+ال substrate اللى هيشتغل عليها ال enzyme هو ال
+
+54
+00:03:56,180 --> 00:03:59,860
+nitrate بالنسبة للنواتج التفاعل ممكن يكون ال
+
+55
+00:03:59,860 --> 00:04:03,100
+nitrate اتوقف لها فقط يكون عندها enzyme ال nitrate
+
+56
+00:04:03,100 --> 00:04:06,660
+تتردكتز وتتوقف لل nitrate ممكن يتم اختزال ال
+
+57
+00:04:06,660 --> 00:04:09,340
+nitrate ل ammonium ion ناتج التفاعل يكون ammonium
+
+58
+00:04:09,340 --> 00:04:12,820
+ion ممكن تعمل complete reduction فبالتالي يكون
+
+59
+00:04:12,820 --> 00:04:15,360
+ناتج التفاعل molecular nitrogen
+
+60
+00:04:21,120 --> 00:04:24,360
+Nitrate reduction test لبرنسيب اللي قلنا انه ال
+
+61
+00:04:24,360 --> 00:04:27,000
+Nitrate هي ال substrate اللي هيشتغل عليها الانزيم
+
+62
+00:04:27,000 --> 00:04:31,000
+هكشف عن انزيم ال Nitrate Reductase هيشتغل على ال
+
+63
+00:04:31,000 --> 00:04:35,180
+Nitrate هيتم اختزاله ل Nitrite قلنا ممكن بعض
+
+64
+00:04:35,180 --> 00:04:40,420
+البكتيريا انها تعمل اختزال لل Nitrite لأمونيوم
+
+65
+00:04:40,420 --> 00:04:45,840
+عشان تحصل على نيتروجين source برضه ممكن يتم يتم
+
+66
+00:04:45,840 --> 00:04:50,500
+اختزال ال Nitrate لموليكولار نيتروجين زي ما شفنا
+
+67
+00:04:50,500 --> 00:04:53,840
+في ال slide اللي قبل كيف سلسلة التفاعلات اللي
+
+68
+00:04:53,840 --> 00:04:58,710
+بتصير عشان أحصل بالنهاية على موليكولارالنيتروجين
+
+69
+00:04:58,710 --> 00:05:03,190
+طبعا في سلسلة تفاعلات وهيتم استخدام عدة أنزيمات
+
+70
+00:05:03,190 --> 00:05:06,090
+لكن أول أنزيم هيتم استخدامه الـ Nitrate Reductase
+
+71
+00:05:06,090 --> 00:05:09,790
+يبقى هكشف على الـ Nitrate Reductase في حال تم
+
+72
+00:05:09,790 --> 00:05:13,250
+اختزال الـ Nitrate لـ Molecular Nitrogen لأن هو
+
+73
+00:05:13,250 --> 00:05:17,370
+أول أنزيم هيشتغل على الـ Nitrate بعدين هيحوله لـ
+
+74
+00:05:17,370 --> 00:05:20,850
+Nitrate بعدين الـ Nitrate هيتم اختزالها باستخدام
+
+75
+00:05:20,850 --> 00:05:24,830
+أنزيم Nitrate Reductase حتى بالنهاية أصل لـ
+
+76
+00:05:24,830 --> 00:05:28,910
+Molecular Nitrogenيبقى سواء كان ناتج تفاعل نيترات
+
+77
+00:05:28,910 --> 00:05:32,650
+او ناتج تفاعل امونيوم او molecular nitrogen هذا
+
+78
+00:05:32,650 --> 00:05:37,730
+يعني ان البكتيريا عندها انزيم النيترات ريدكتاز لكن
+
+79
+00:05:37,730 --> 00:05:41,290
+هان بيصير في عندي complete reduction هان لأ فقط
+
+80
+00:05:41,290 --> 00:05:46,670
+للنيترات بعدين ممكن انه يتم الاستفادة منه اختزاله
+
+81
+00:05:46,670 --> 00:05:50,950
+لامونيوم حتى احصل على ال nitrogen source بالنسبة
+
+82
+00:05:50,950 --> 00:05:56,190
+لل procedure كيف بدي اكشف عن هذا الانزيمأكيد هجيب
+
+83
+00:05:56,190 --> 00:05:59,770
+ميديا يكون فيها ال substrate اللى هيشتغل فيه عليها
+
+84
+00:05:59,770 --> 00:06:03,850
+الانزيم ال media اسمها نيترات ال broth بتكون ال
+
+85
+00:06:03,850 --> 00:06:08,410
+broth فبالتالي طريقة الزراعة باخد انيكولام باخد
+
+86
+00:06:08,410 --> 00:06:11,090
+البكتيريا اللى بدي أكشف هل عندها الانزيم ولا لأ
+
+87
+00:06:11,090 --> 00:06:15,270
+بعمل mix فيه ال broth هاي ال نيترات ال broth بقولى
+
+88
+00:06:15,270 --> 00:06:20,810
+بتحتوي على نصف المية KNO3 يبقى ال substrate اللى
+
+89
+00:06:20,810 --> 00:06:24,710
+هيشتغل عليها الانزيم ال NO3بتكون موجودة في هذه
+
+90
+00:06:24,710 --> 00:06:29,130
+الميديا بعد ما أزرع البكتيري هعمل incubation لمدة
+
+91
+00:06:29,130 --> 00:06:35,910
+24 ساعة على 37 درجة سليسيا في ال incubatorهلأ لو
+
+92
+00:06:35,910 --> 00:06:41,470
+البكتيريا عندها أنزيم النيترات Reductase ممكن انه
+
+93
+00:06:41,470 --> 00:06:45,470
+يكون ناتج التفاعل نيترات ممكن يكون أمانيا ممكن
+
+94
+00:06:45,470 --> 00:06:51,250
+يكون molecular نيتروجين كيف بدي أكشف عن هدولة بقول
+
+95
+00:06:51,250 --> 00:06:56,820
+بتضيف بعد الincubation reagentزي ما قلنا اي تفاعل
+
+96
+00:06:56,820 --> 00:07:00,840
+كيف بدى اكشف عنه اما بشوفه باعينى او ممكن اضيف بيه
+
+97
+00:07:00,840 --> 00:07:04,760
+H Indicator اذا كان ناتج التفاعل acid او base
+
+98
+00:07:04,760 --> 00:07:10,120
+فبالتالي هيغير لون الوسط او ممكن اضيف reagent هذا
+
+99
+00:07:10,120 --> 00:07:16,530
+ال reagent بيتفاعل مع احد نواتج التفاعل تمامفهنا
+
+100
+00:07:16,530 --> 00:07:20,330
+طبعا ماعندي ازد ولا بيز مش هقدر ايه اشوف ناتج
+
+101
+00:07:20,330 --> 00:07:23,990
+التفاعل بقى يعني ماعندي جاز مثلا فبالتالي هضيف
+
+102
+00:07:23,990 --> 00:07:27,850
+reactant هذا ال reactant هيتفاعل مع احد نواتج
+
+103
+00:07:27,850 --> 00:07:33,570
+التفاعل بقوللي بضيف بعد ال incubation زي فحص ال
+
+104
+00:07:33,570 --> 00:07:37,010
+methyl red vocosper scour كنا نضيف ال reactant بعد
+
+105
+00:07:37,010 --> 00:07:40,210
+ال incubation نعمل زراعة و نضيف بعد ال incubation
+
+106
+00:07:40,210 --> 00:07:44,670
+هذا نفس الفكرة نضيف بعد ال incubationReagent A
+
+107
+00:07:44,670 --> 00:07:49,750
+اسمه Sulphanilic Acid Reagent B بضيفه Diamethyl
+
+108
+00:07:49,750 --> 00:07:54,010
+Nafthalamine بضيف خمسة drop من Reagent A وخمسة
+
+109
+00:07:54,010 --> 00:08:00,830
+drop من Reagent B وبشوف التفاعل بقوللي ان Reagent
+
+110
+00:08:00,830 --> 00:08:06,970
+A و Reagent B بيتفاعلوا مع ال Nitrite بيتفاعلوا مع
+
+111
+00:08:06,970 --> 00:08:11,490
+ال Nitrite قولنا أي Reagent بضيفه بيتفاعل مع نواتج
+
+112
+00:08:11,490 --> 00:08:15,420
+التفاعلreagent A و B بتفاعل مع مين بالظبط من نواتج
+
+113
+00:08:15,420 --> 00:08:21,820
+التفاعل بتفاعل مع ال nitrite بقول لي لو ال nitrite
+
+114
+00:08:21,820 --> 00:08:24,560
+لو تم اغتزال ال nitrite ل Nitrite و ال nitrite
+
+115
+00:08:24,560 --> 00:08:29,280
+موجود هيرتبط ب reagent A و B و هيعطيني red complex
+
+116
+00:08:29,280 --> 00:08:35,660
+فبالتالي لو اتحول لون ال meat يبعد إضافة reagent A
+
+117
+00:08:35,660 --> 00:08:39,880
+و B للون ال red معناه هاي ان البكتيريا عندها أنزيم
+
+118
+00:08:39,880 --> 00:08:45,010
+ال nitrate reductaseحولت ال nitrate ل nitrite يبقى
+
+119
+00:08:45,010 --> 00:08:49,070
+high positive من الأمثلة عليها ال E coli انها حولت
+
+120
+00:08:49,070 --> 00:08:52,290
+ال nitrate ل nitrite ال nitrite اللى فى ال media
+
+121
+00:08:52,290 --> 00:08:56,030
+اتفاعل مع ال reagent a و b اللى انا ضفتهم اعطانى
+
+122
+00:08:56,030 --> 00:09:01,490
+red complex طيب لما اتغير اللون بقولى ما بقدر اكتب
+
+123
+00:09:01,490 --> 00:09:06,110
+انه negative ما بقدر احكى ان هذه النتيجة negative
+
+124
+00:09:06,110 --> 00:09:11,180
+ليش لأن احنا قلناإن ناتج التفاعل أو البكتيريا اللي
+
+125
+00:09:11,180 --> 00:09:13,720
+عندها أنزيم الـNitrate Reductase ممكن يكون ناتج
+
+126
+00:09:13,720 --> 00:09:19,020
+التفاعل Nitrite ممكن بعد ما يتم يكون الـNitrite في
+
+127
+00:09:19,020 --> 00:09:23,040
+الميديا يتم اختزاله لأمونيوم ممكن برضه إنه يصير في
+
+128
+00:09:23,040 --> 00:09:26,880
+عندي Complete Reduction ويتم تحويل الـNitrate عبر
+
+129
+00:09:26,880 --> 00:09:30,480
+سلسلة من الإنزيمات وسلسلة من التفاعلات لموليكولر
+
+130
+00:09:30,480 --> 00:09:36,540
+نيتروجينفبالتالي لو طلع النتيجة colorless هذا
+
+131
+00:09:36,540 --> 00:09:40,300
+لايعني انه negative ممكن يكون فعلا البكتيريا ما
+
+132
+00:09:40,300 --> 00:09:44,480
+عندها الانزيموممكن ان البكتيريا يكون عندها الانزيم
+
+133
+00:09:44,480 --> 00:09:49,360
+لكن حولت النيتريات لأمونيوم او حولت النيتريات
+
+134
+00:09:49,360 --> 00:09:53,080
+لموليكيولا نيتروجين في صار عندها complete
+
+135
+00:09:53,080 --> 00:09:58,180
+reduction طيب كيف بدي اعرف هل صار في عندي complete
+
+136
+00:09:58,180 --> 00:10:02,960
+reduction او اتحول لأمونيوم او فعلا البكتيريا ما
+
+137
+00:10:02,960 --> 00:10:06,860
+عندها انزيم النيتريات الريدكتاز بقوللي بضيفي
+
+138
+00:10:06,860 --> 00:10:12,280
+reaction C في حالكان اللون colorless ما اتغير بعد
+
+139
+00:10:12,280 --> 00:10:16,880
+إضافة reagent a و b بتضيفي reagent c اللي هو ال
+
+140
+00:10:16,880 --> 00:10:21,760
+zinc powder ال zinc powder يعتبر reducing agent
+
+141
+00:10:21,760 --> 00:10:27,620
+طبعا هضيف reagent c بقوللي بعد إضافة reagent c في
+
+142
+00:10:27,620 --> 00:10:32,520
+حال كانت النتيجة colorless بعد إضافة reagent a و b
+
+143
+00:10:32,520 --> 00:10:37,680
+إذا اللون اتحول للون red معناه هي النتيجة negative
+
+144
+00:10:38,400 --> 00:10:43,660
+لكن لو ما اتغير معناه هذه النتيجة انها positive
+
+145
+00:10:43,660 --> 00:10:47,220
+صار عندي complete reduction اتحول ال nitrate ل
+
+146
+00:10:47,220 --> 00:10:50,620
+molecular نيتروجين او ال nitrate بعد ما اتحول ل
+
+147
+00:10:50,620 --> 00:10:54,420
+nitrite اتحول ل ammonia طيب ليش احنا قولنا ان
+
+148
+00:10:54,420 --> 00:10:58,080
+النتيجة ال red negative و هنقولنا ان النتيجة ال
+
+149
+00:10:58,080 --> 00:11:02,800
+red positive الفكرة ان ال zinc powder يعتبر
+
+150
+00:11:02,800 --> 00:11:08,300
+reducing agent ايش يعني reducing agentيعني هيشتغل
+
+151
+00:11:08,300 --> 00:11:12,340
+شغل البكتيريا اللى عندها انزيم الـ Nitrate
+
+152
+00:11:12,340 --> 00:11:17,040
+Reductase لو البكتيريا ما عندها الانزيم هان الـ
+
+153
+00:11:17,040 --> 00:11:19,620
+negative لو ما عندها انزيم الـ Nitrate Reductase
+
+154
+00:11:19,620 --> 00:11:24,380
+هذا يعني انه ماصار تفاعل النيترات دلوا موجود في
+
+155
+00:11:24,380 --> 00:11:28,740
+الميديا السبسترات دلت موجودة في الميديا ماصار في
+
+156
+00:11:28,740 --> 00:11:33,020
+عندي اي تفاعل النيترات ما اتفاعل دلت موجودة في
+
+157
+00:11:33,020 --> 00:11:37,640
+الميدياهيجيز zinc powder اللى يعتبر reducing agent
+
+158
+00:11:37,640 --> 00:11:42,540
+هيشتغل شغل الانزيم هيحول ال nitrate اللى موجودة في
+
+159
+00:11:42,540 --> 00:11:48,460
+ال media لمعصر فيها اى تفاعل ل nitrite ال nitrite
+
+160
+00:11:48,460 --> 00:11:51,360
+تمام هيك صار عندى nitrite موجودة في ال media ال
+
+161
+00:11:51,360 --> 00:11:55,380
+nitrite انا ضفت قبلها reagent a و b سابقا كان مضعف
+
+162
+00:11:55,380 --> 00:12:00,530
+reagent a و b لما صار colorlessهيتفاعلوا reaction
+
+163
+00:12:00,530 --> 00:12:06,590
+a و b مع الـ nitrite اللى تم اختزاله بواسطة ال
+
+164
+00:12:06,590 --> 00:12:11,810
+zinc powder و ليس بواسطة البكتيريا لأنه ليش تم
+
+165
+00:12:11,810 --> 00:12:18,050
+تحويله لأن ال nitrate لم يتم استهلاكها كانت
+
+166
+00:12:18,050 --> 00:12:20,850
+البكتيريا معاها أنزيم ال nitrate reductase فضلت
+
+167
+00:12:20,850 --> 00:12:24,010
+موجودة في ال media فبالتالي هيش ال zinc powder
+
+168
+00:12:24,010 --> 00:12:28,380
+هيعملها reduction لل nitriteهيتفاعل مع reactant A
+
+169
+00:12:28,380 --> 00:12:32,340
+و B الموجود سابقاً فهيتحول اللون للون Red فبالتالي
+
+170
+00:12:32,340 --> 00:12:36,640
+اللون ال Red بعد إضافة reactant C اللي هو ال zinc
+
+171
+00:12:36,640 --> 00:12:40,880
+powder يعتبر negative من الأمثلة على البكتيريا I
+
+172
+00:12:40,880 --> 00:12:44,080
+اللي ما عندها الانزيم النيترات ريداكتاز اللي هو
+
+173
+00:12:44,080 --> 00:12:51,480
+لستاف Epidermidis طيب بالنسبة للبعد إضافة Reagent
+
+174
+00:12:51,480 --> 00:12:55,060
+C إذا دلت colorless قولنا هذا يعني انه بتعمل
+
+175
+00:12:55,060 --> 00:12:58,720
+complete reduction او حولت ال nitrate ل ammonia
+
+176
+00:12:58,720 --> 00:13:04,490
+لسه عندي colorlessلأن reagent C هو يعتبر reducing
+
+177
+00:13:04,490 --> 00:13:09,570
+agent هيشتغل على النيترات طيب في حال كان عندي
+
+178
+00:13:09,570 --> 00:13:13,470
+complete reduction النيترات موجود في الميديا لأ تم
+
+179
+00:13:13,470 --> 00:13:17,630
+استهلاكه تم تحويله لموليكيولر نيتروجين أو تم
+
+180
+00:13:17,630 --> 00:13:21,830
+تحويله لأمونيام فبالتالي النيترات اللي هيشتغل
+
+181
+00:13:21,830 --> 00:13:25,870
+عليها ال zinc powder مش موجود فبالتالي مش هيصير اي
+
+182
+00:13:25,870 --> 00:13:29,710
+تفاعل مش هيتحول النيترات لنيترات مش هيرتبط ب
+
+183
+00:13:29,710 --> 00:13:34,210
+reagent اوأو بي مش هيتحول اللون للون أحمر فبالتالي
+
+184
+00:13:34,210 --> 00:13:38,830
+ال colorless بعد إضافة reaction C يعتبر معناه إنه
+
+185
+00:13:38,830 --> 00:13:41,890
+البكتيريا عملت complete reduction حولت ال nitrate
+
+186
+00:13:41,890 --> 00:13:46,230
+ل molecular nitrogen أو أمونيا من الأمثلة على
+
+187
+00:13:46,230 --> 00:13:49,270
+البكتيريا اللي بتعمل complete reduction الصدوموناس
+
+188
+00:13:49,270 --> 00:13:55,300
+يبقى الصدوموناس لو أنا ضفت reaction A أو Bهتعطيني
+
+189
+00:13:55,300 --> 00:13:59,940
+colorless لكن بعد إضافة reagent C هتظلها colorless
+
+190
+00:13:59,940 --> 00:14:04,580
+ال E.coli فقط هتعمل reduction للنيترات مش هتعمل
+
+191
+00:14:04,580 --> 00:14:07,500
+complete reduction عشان هيك لما أضيف reagent A و B
+
+192
+00:14:07,500 --> 00:14:10,540
+بسرعة هلاحظ أنه في عندي لون أحمر هذا معناه أن
+
+193
+00:14:10,540 --> 00:14:14,280
+البكتيريا عندها أنزيم النيتريات Reductase لكن
+
+194
+00:14:14,280 --> 00:14:17,200
+ماعملت complete reduction فقط حولت النيتريات
+
+195
+00:14:17,200 --> 00:14:21,140
+لنيترات من الأمثلة عليها ال E.coli بعد إضافة
+
+196
+00:14:21,140 --> 00:14:25,680
+reagent C إذا كان اللون Redهذا معناه ان ما عندها
+
+197
+00:14:25,680 --> 00:14:28,820
+الانزيم من الأمثلة عليها الـ Staph Epidermis ليش
+
+198
+00:14:28,820 --> 00:14:32,880
+اتحول اللون للون Red لأن Zinc Powder اشتغل شغل
+
+199
+00:14:32,880 --> 00:14:37,680
+الانزيم اشتغل على ال Nitrate اللي لم يتم استهلاكها
+
+200
+00:14:37,680 --> 00:14:40,660
+لأنها ما عندها الانزيم فضلت موجودة في ال media
+
+201
+00:14:40,660 --> 00:14:44,380
+اشتغل عليها ال Zinc Powder يبقى اللون ال Red كان
+
+202
+00:14:44,380 --> 00:14:48,260
+ناتج من ال Zinc Powder اللي يعتبر reducing agent
+
+203
+00:14:48,260 --> 00:14:54,460
+وليس من البكتيريافاشتغل عليها و اعطاني ال nitrite
+
+204
+00:14:54,460 --> 00:14:57,520
+ال nitrite اتفاعل مع ال reagent a و b اللى موجود
+
+205
+00:14:57,520 --> 00:15:00,640
+سابقا و اعطاني اللون ال red لكن ال zinc powder
+
+206
+00:15:00,640 --> 00:15:04,520
+مالاقى اشي في حال ال complete reduction مالاقى ال
+
+207
+00:15:04,520 --> 00:15:07,520
+nitrate عشان يشتغل عليها فبالتالي مش هيصير اي
+
+208
+00:15:07,520 --> 00:15:10,600
+تفاعل لأنه صار عندي complete reduction اتحول ال
+
+209
+00:15:10,600 --> 00:15:14,860
+nitrate ل molecular nitrogen فمش موجود ال nitrate
+
+210
+00:15:14,860 --> 00:15:20,410
+تم استهلاكه في التفاعلهذا بالنسبة للـ procedure و
+
+211
+00:15:20,410 --> 00:15:23,890
+أمثلة على البكتيريا اللى بتتحول فقط الـ nitrate ل
+
+212
+00:15:23,890 --> 00:15:28,710
+nitrite أمثلة على البكتيريا اللى بتتحول ال nitrate
+
+213
+00:15:28,710 --> 00:15:31,250
+ل molecular nitrogen بتعمل complete reduction
+
+214
+00:15:31,250 --> 00:15:34,410
+الصدمه و نصب البكتيريا اللى ما عندها أنزيم للـ
+
+215
+00:15:34,410 --> 00:15:38,490
+Staphylococcus epidermidisهذا ملخص فحص الـ Nitrate
+
+216
+00:15:38,490 --> 00:15:42,550
+Reduction Test الـ Principle أشهر الـ procedure من
+
+217
+00:15:42,550 --> 00:15:46,090
+الـ positive و الـ negative للفحص يبقى لو جيبنا
+
+218
+00:15:46,090 --> 00:15:52,350
+سؤال صح و غلط أنه بعد إضافة Reagent C اللون إذا
+
+219
+00:15:52,350 --> 00:15:57,130
+اتحول للون أحمر هذا معناه أن النتيجة positive غلط
+
+220
+00:15:57,500 --> 00:16:00,960
+بعد إضافة Reagency إذا اتحول اللون للون أحمر هذا
+
+221
+00:16:00,960 --> 00:16:05,640
+معناه إنه نتيجة negative اللون الأحمر نتج نتيجة
+
+222
+00:16:05,640 --> 00:16:10,460
+إنه تم اختزال ال Nitrate ل Nitrite باستخدام ال
+
+223
+00:16:10,460 --> 00:16:15,360
+Zinc Powder وليس بسبب البكتيريا أو الإنزيم الموجود
+
+224
+00:16:15,360 --> 00:16:21,390
+في البكتيريانمر على ال slide ع السريع organism
+
+225
+00:16:21,390 --> 00:16:24,850
+that poses the enzyme nitrate reductase البكتيريا
+
+226
+00:16:24,850 --> 00:16:28,290
+اللي عندها أنزيم النيترات ريدكتاز هتعمل اختزال من
+
+227
+00:16:28,290 --> 00:16:32,550
+النيترات لنيترات قلنا النيترات هو ال substrate
+
+228
+00:16:32,550 --> 00:16:36,310
+لأنزيم النيترات الريدكتاز بقول النيترات إذا
+
+229
+00:16:36,310 --> 00:16:39,830
+ملاحظين هي النيترات كيف بدي أكشف عنه قلنا انه
+
+230
+00:16:39,830 --> 00:16:44,200
+reagent a و bهيتفاعلوا فقط مع النيترات مع ناتج
+
+231
+00:16:44,200 --> 00:16:47,100
+التفاعل النيترات وليس مع ال ammonium وليس مع ال
+
+232
+00:16:47,100 --> 00:16:51,780
+molecular nitrogen فبالتالي لو كانت النتيجة ما
+
+233
+00:16:51,780 --> 00:16:55,640
+تغير اللون هذا لا يعني انه ما عندها الانزيم ممكن
+
+234
+00:16:55,640 --> 00:16:59,290
+انها تكون عاملة complete reductionمش موجود اندي
+
+235
+00:16:59,290 --> 00:17:03,630
+ناتج التفاعل نيتريت موجود موليكولر نيتروجين او
+
+236
+00:17:03,630 --> 00:17:08,250
+امونيام فبالتالي ماتفعلوا مع reagent A و B فهضيف
+
+237
+00:17:08,250 --> 00:17:11,590
+اللي هو ال zinc powder كيف بدي اكشف على ال nitrite
+
+238
+00:17:11,590 --> 00:17:16,250
+ion بقول بإضافة sulfanilic acid reagent A ما
+
+239
+00:17:16,250 --> 00:17:20,620
+ذكرناه اللي هو يعتبراسمه salphanilic acid reagent
+
+240
+00:17:20,620 --> 00:17:24,980
+باسمه الـ dimethyl naphthalamide يبقىها reagent A
+
+241
+00:17:24,980 --> 00:17:28,360
+salphanilic acid reagent B dimethyl naphthalamide
+
+242
+00:17:28,360 --> 00:17:32,800
+هضيفهم على المزرعة بعد ال incubation 24 ساعة
+
+243
+00:17:32,800 --> 00:17:37,340
+بقوللي any nitrite in the media أي نيتريت موجود في
+
+244
+00:17:37,340 --> 00:17:40,520
+ال media هيتفاعل مع هذه ال reagent وهيعطيني red
+
+245
+00:17:40,520 --> 00:17:44,520
+color يبقىبعد إضافة reagent A و B إذا اتحول اللون
+
+246
+00:17:44,520 --> 00:17:49,620
+للون Red معناه هاي أنه تم تحويل ال Nitrate ل
+
+247
+00:17:49,620 --> 00:17:54,360
+Nitrite باستخدام إنزيم ال Nitrate Reductase هذه
+
+248
+00:17:54,360 --> 00:17:57,860
+الصورة للتفاعل قلنا ال Nitrate هي ال substrate هي
+
+249
+00:17:57,860 --> 00:18:01,260
+تم اختزالها ل Nitrite ممكن ال Nitrate بعض أنواع
+
+250
+00:18:01,260 --> 00:18:05,140
+البكتيريا تعملوا اختزال ل Ammonium وممكن يصير في
+
+251
+00:18:05,140 --> 00:18:08,410
+عندي complete reductionأخر احصل على molecular
+
+252
+00:18:08,410 --> 00:18:11,970
+nitrogen يعني في النهاية هحصل على nitrogen source
+
+253
+00:18:11,970 --> 00:18:16,830
+لازم اللي هو البكتيريا بقولي لو البكتيريا ما تغير
+
+254
+00:18:16,830 --> 00:18:21,970
+لونها مصر فين دي اي تغير في اللون في بعد إضافة
+
+255
+00:18:21,970 --> 00:18:26,230
+reagent a و b هذا معناه ان البكتيرياThe bacteria
+
+256
+00:18:26,230 --> 00:18:29,350
+bosses nitrate reductase عندها البكتيريا نيترات
+
+257
+00:18:29,350 --> 00:18:34,630
+ردكتاز لكن عملت reduce لالنيترات لأمونيوم قلنا
+
+258
+00:18:34,630 --> 00:18:37,810
+ممكن بعد ما يتم اختزال النيترات لنيترات يتم
+
+259
+00:18:37,810 --> 00:18:42,070
+اختزاله لأمونيوم أو ممكن انه يتم اختزاله ل
+
+260
+00:18:42,070 --> 00:18:46,480
+molecular نيتروجين في سلسلة من التفاعلاتهذه نفس
+
+261
+00:18:46,480 --> 00:18:49,760
+الفكرة انه عندها الانزيم لكن عملت اختيار للنيترات
+
+262
+00:18:49,760 --> 00:18:54,620
+لامونيا او الاحتمال التالت انه البكتيريا ما عندها
+
+263
+00:18:54,620 --> 00:18:58,480
+انزيم النيترات Reductase فبالتالي ما صار reduction
+
+264
+00:18:58,480 --> 00:19:05,350
+للنيترات بقول لي عشانأحدد هل البكتيريا عندها
+
+265
+00:19:05,350 --> 00:19:09,850
+الانزيم عملت complete reduction أو حولته لأمونيوم
+
+266
+00:19:09,850 --> 00:19:13,810
+أو فعلا هي ما عندها الانزيم هضيف ال zinc powder a
+
+267
+00:19:13,810 --> 00:19:16,190
+small amount of zinc powder is added to the
+
+268
+00:19:16,190 --> 00:19:20,050
+culture containing the reagent هيه هضيف ال zinc
+
+269
+00:19:20,050 --> 00:19:24,810
+powder للمزرعة اللي بتحتوي على reagent A وB سابقا
+
+270
+00:19:25,450 --> 00:19:29,050
+بقولي الـ Zinc إيش وظيفته؟ الـ Zinc Powder يُعتبر
+
+271
+00:19:29,050 --> 00:19:32,710
+قولنا الـ Reducing Agent Reduce نيتريات و نيتريات
+
+272
+00:19:32,710 --> 00:19:36,730
+قولنا هيعمل اختزال للنيتريات اللي موجود في الميديا
+
+273
+00:19:36,730 --> 00:19:42,250
+لنيتريات فبالتالي a pink أو red color أبير هيتحول
+
+274
+00:19:42,250 --> 00:19:46,350
+لون الوسط للون أحمر لأنه تم اختزال النيتريات
+
+275
+00:19:46,350 --> 00:19:49,890
+لنيتريات بواسطة الـ Zinc Powder وليس بواسطة
+
+276
+00:19:49,890 --> 00:19:53,510
+البكتيريا الـ Nitrite موجود في الميديا قولنا الـ
+
+277
+00:19:53,510 --> 00:19:57,140
+Nitriteبرتبط بـ reagent A و B الموجودين في الميديا
+
+278
+00:19:57,140 --> 00:20:01,160
+فيعطيين اللون الأحمر بقولي الحقيقة أن الـ Nitrate
+
+279
+00:20:01,160 --> 00:20:05,280
+were not reduced to nitrite by the bacteria في هذه
+
+280
+00:20:05,280 --> 00:20:08,860
+الحالة أن الـNitrate لم يتم اختزاله لـNitrite
+
+281
+00:20:08,860 --> 00:20:12,300
+باستخدام البكتيريا تم اختزاله باستخدام الـ Zinc
+
+282
+00:20:12,300 --> 00:20:16,800
+powder طيب ليش البكتيريا ليش ال Zinc powder قدر
+
+283
+00:20:16,800 --> 00:20:19,460
+انه يختزل من ال Nitrate لـNitrite؟ بقولي لأن
+
+284
+00:20:19,460 --> 00:20:24,830
+الـNitrate لم يتم استهلاكه Nitrate unreactedلم يتم
+
+285
+00:20:24,830 --> 00:20:30,050
+استهلاكه فهذا يعني ان البكتيريا ما عندها انزيم ال
+
+286
+00:20:30,050 --> 00:20:34,910
+nitrate reductase لكن ال red color doesn't appear
+
+287
+00:20:34,910 --> 00:20:38,730
+اذا ما ظهر اللون الأحمر هذا يعني ان ال nitrate
+
+288
+00:20:38,730 --> 00:20:42,810
+الموجود في ال media تم اختزاله سابقا ل nitrite
+
+289
+00:20:43,250 --> 00:20:46,750
+بعدها تم اختزاله ل ammonium or nitrogen gas
+
+290
+00:20:46,750 --> 00:20:50,690
+فبالتالي هان انه ال nitrate ال substrate تم
+
+291
+00:20:50,690 --> 00:20:54,810
+استهلاكها فلما ييجي ال zinc powder هيشتغل عليها مش
+
+292
+00:20:54,810 --> 00:20:57,690
+هلاقى مش هيلاقي اشي يشتغل عليها فبالتالي مش هيصير
+
+293
+00:20:57,690 --> 00:21:01,330
+اي تفاعل مش هيصير اي تغير في اللون فهذا يعني انه
+
+294
+00:21:01,330 --> 00:21:05,090
+صار في عندي complete reduction او اتحول ال nitrate
+
+295
+00:21:05,090 --> 00:21:10,810
+تم اختزاله ل ammoniumكيف ال procedure قلنا بستخدم
+
+296
+00:21:10,810 --> 00:21:14,470
+نيترات ال breath فيها بوتاسيوم نيترات نصف المية
+
+297
+00:21:14,470 --> 00:21:19,010
+بعمل incubation هضيف خمسة drop من reactant A وخمسة
+
+298
+00:21:19,010 --> 00:21:24,070
+drop من reactant B بقولي if nitrate is present in
+
+299
+00:21:24,070 --> 00:21:27,850
+the media it will turn red within one to two
+
+300
+00:21:27,850 --> 00:21:33,090
+minutes يعني ان في غلط في ال slide نغيرها ل
+
+301
+00:21:33,090 --> 00:21:37,420
+nitriteلو الـ Nitrite موجودة في الميديا قلنا
+
+302
+00:21:37,420 --> 00:21:40,760
+هتتفاعل مع reagent A و B و هتعطيني اللون الأحمر
+
+303
+00:21:40,760 --> 00:21:45,000
+يبقى ال positive هي red color بعد إضافة reagent A
+
+304
+00:21:45,000 --> 00:21:52,060
+و B لو ما تغير قلنا مابنحكي negative مابنحكي
+
+305
+00:21:52,060 --> 00:21:56,040
+negative ال negative test بعد إضافة reagent A و B
+
+306
+00:21:56,040 --> 00:22:00,840
+قلنا مانضيف zinc powder لو اتحول اللون للون الأحمر
+
+307
+00:22:00,840 --> 00:22:05,380
+هذا معناه إنه نتيجة negativeلأن الذنك بودر اشتغل
+
+308
+00:22:05,380 --> 00:22:08,900
+على الـ Nitrate اللي لم يتم استهلاكه لأن البكتيريا
+
+309
+00:22:08,900 --> 00:22:14,040
+ما عندها انزيم الـ Nitrate Reductase لكن النتيجة
+
+310
+00:22:14,040 --> 00:22:16,940
+الـ Positive معناه انه عملت البكتيريا Complete
+
+311
+00:22:16,940 --> 00:22:20,860
+Reduction او تم اختزال الـ Nitrate لأمونيوم
+
+312
+00:22:20,860 --> 00:22:24,260
+فبالتالي اجى الذنك بودر هيشتغل على الـ Nitrate ما
+
+313
+00:22:24,260 --> 00:22:28,520
+لقى الـNitrate تم استهلاكها فبالتالي ماهيصير تفاعل
+
+314
+00:22:28,520 --> 00:22:33,450
+هيكون Colorless يبقى هنعدل انهمش الـ Nitrate لو
+
+315
+00:22:33,450 --> 00:22:36,590
+الـ Nitrate موجود في الـ media هيتحول اللون للون
+
+316
+00:22:36,590 --> 00:22:43,730
+أحمر لأن هو اللي هيرتبط بـ reagent A و B هان
+
+317
+00:22:43,730 --> 00:22:46,550
+التفاعل احنا قلنا في هندي أكتر من احتمال ممكن
+
+318
+00:22:46,550 --> 00:22:50,430
+البكتيريا يتحول ال Nitrate ل Nitrite ممكن يتم
+
+319
+00:22:50,430 --> 00:22:54,630
+تحويل ال Nitrate ل molecular nitrogen لإنه تعمل
+
+320
+00:22:54,630 --> 00:22:58,450
+complete reduction قلنا ممكن بعد ما يتم اختزال ال
+
+321
+00:22:58,450 --> 00:23:03,080
+Nitrate ل Nitrite انهايختزل الـ Nitrate لـ Ammonia
+
+322
+00:23:03,080 --> 00:23:06,800
+يعني يكون التفاعل اختزال الـ Nitrate لـ Ammonia
+
+323
+00:23:06,800 --> 00:23:09,220
+ممكن مايصير في نتيجة الـ Negative هذه التفاعل هذه
+
+324
+00:23:09,220 --> 00:23:15,100
+النتيجة الـ Positive هذا معناه أنه عندها الانزيم
+
+325
+00:23:15,100 --> 00:23:21,000
+لكن نتائج التفاعل بتختلف هنا نتيجة التفاعل Nitrate
+
+326
+00:23:21,000 --> 00:23:26,840
+هنا نيتروجين جاز هنا Ammonia الرابع واحدة هي ما
+
+327
+00:23:26,840 --> 00:23:31,250
+عندها انزيم يبقى نقطةهذه هي الـ Positive
+
+328
+00:24:21,150 --> 00:24:23,390
+لكن هاي اخر issue هي negative
+
+329
+00:24:47,360 --> 00:24:51,440
+يبقى سواء كان ناتج التفاعل نيتريت أو molecular
+
+330
+00:24:51,440 --> 00:24:54,680
+nitrogen أو أمونيوم هذا معناه أن البكتيريا عندها
+
+331
+00:24:54,680 --> 00:24:59,300
+الانزيم لكن هانصار في ياندي complete reduction لو
+
+332
+00:24:59,300 --> 00:25:03,660
+ماصار تفاعل يبقى أو ماعندها البكتيريا الانزيم يبقى
+
+333
+00:25:03,660 --> 00:25:07,140
+ماهيصير تفاعل فبالتالي النيتريات ال substrate هتظل
+
+334
+00:25:07,140 --> 00:25:11,340
+موجودة في ال media أكيد بالنسبة لل nitrogen gas
+
+335
+00:25:11,340 --> 00:25:15,320
+مافيش كلهم موجود لكن في حال كانت عملة البكتيريا
+
+336
+00:25:15,320 --> 00:25:18,460
+complete reduction حولتالنيترات لـ molecular
+
+337
+00:25:18,460 --> 00:25:23,960
+nitrogen في عندي بيكون nitrogen gas بعد ما اضيف
+
+338
+00:25:23,960 --> 00:25:28,580
+reagent A و B يقولنا اذا اتحول اللون للون red هذا
+
+339
+00:25:28,580 --> 00:25:34,300
+معناه انه البكتيريا حولت النيترات لنيترات فقطليش؟
+
+340
+00:25:34,300 --> 00:25:39,420
+لأن قلنا إن الـreagent A وB بيتفاعل مع الـnitrite
+
+341
+00:25:39,420 --> 00:25:43,400
+ما بيتفاعل مع الـnitrogen molecular nitrogen أو
+
+342
+00:25:43,400 --> 00:25:46,620
+الـammonium أو الـnitrate في حال كانت البكتيريا
+
+343
+00:25:46,620 --> 00:25:49,840
+negative يبقى بعد إضافة reagent A وB إذا اتحول
+
+344
+00:25:49,840 --> 00:25:54,680
+اللون للون Red منشوف التفاعل إذا ناتج التفاعل
+
+345
+00:25:54,680 --> 00:25:58,760
+Nitrite يبقى نحكي إنه في عندي هيتحول اللون للون
+
+346
+00:25:58,760 --> 00:26:01,940
+Red لكن لو كان ناتج التفاعل مش Nitrite يبقى كلهم
+
+347
+00:26:01,940 --> 00:26:05,350
+هانمش ناتج التفاعل نيتريت مش ناتج التفاعل نيتريت
+
+348
+00:26:05,350 --> 00:26:11,530
+مش عندى تفاعل المواد المتفاعلة نيتريت مش نيتريت
+
+349
+00:26:11,530 --> 00:26:14,750
+يبقى مش هيصير ثغير فى اللون لأن ال reagent A و B
+
+350
+00:26:14,750 --> 00:26:18,510
+بيرتبط مع ال نيتريت فقط فى حال كان ناتج التفاعل
+
+351
+00:26:18,510 --> 00:26:24,090
+نيتريت هو هحكي هان انه هيتحول اللون للون Red طيب
+
+352
+00:26:24,090 --> 00:26:28,250
+بقولى بعد مضيف ال zinc powder أكيد فى حال كان
+
+353
+00:26:28,250 --> 00:26:32,500
+البكتيريا حولت ال نيتريت لنيتريتما بضيف الذنك
+
+354
+00:26:32,500 --> 00:26:36,420
+باودر فقط بضيف الذنك باودر في حال ما تغير اللون
+
+355
+00:26:36,420 --> 00:26:40,080
+بعد إضافة reagent A وB في هذه الحالة ما بضيف الذنك
+
+356
+00:26:40,080 --> 00:26:43,860
+باودر بعتبرها positive على طول لكن في حال ما تغير
+
+357
+00:26:43,860 --> 00:26:47,340
+اللون بضيف الذنك باودر عشان أفرق من البكتيريا اللي
+
+358
+00:26:47,340 --> 00:26:50,020
+ما عندها ال enzyme ومن البكتيريا اللي عملت
+
+359
+00:26:50,020 --> 00:26:54,110
+complete reductionبعد إضافة الزنك باودر إذا اتحول
+
+360
+00:26:54,110 --> 00:26:58,190
+اللون للون pink to red هاي معناه إنه البكتيريا ما
+
+361
+00:26:58,190 --> 00:27:02,430
+عندها الانزيم لأنه قلنا زنك باودر بيشتغل على قياشة
+
+362
+00:27:02,430 --> 00:27:07,430
+بيشتغل على النيترات فوان هشوف نيترات يبقى هيتحول
+
+363
+00:27:07,430 --> 00:27:11,090
+اللون للون red لكن هان في حال كانت عملت complete
+
+364
+00:27:11,090 --> 00:27:14,350
+reduction أو في حال اتحول النيترات لأمونيوم أكيد
+
+365
+00:27:14,350 --> 00:27:20,050
+بعد إضافة زنك باودر مش هيتغير اللون عنديلأنه مش
+
+366
+00:27:20,050 --> 00:27:23,730
+هيشتغل مش موجود ال substrate اللي هي ال nitrate
+
+367
+00:27:23,730 --> 00:27:26,970
+اللي هيشتغل عليها فبالتالي مش هيصير تفاعل لكن في
+
+368
+00:27:26,970 --> 00:27:30,970
+حالة ال negative أكيد ال nitrate لم يتم استهلاك
+
+369
+00:27:30,970 --> 00:27:34,530
+هضلت في الموجودة في ال media هيجي ال zinc powder
+
+370
+00:27:34,530 --> 00:27:38,010
+اللي هيشتغل نفس شغل الإنزيم هيحولها ل Nitrite ال
+
+371
+00:27:38,010 --> 00:27:41,930
+nitrite هترتبط مع ال agent A و B اللي مضاعف سابقا
+
+372
+00:27:41,930 --> 00:27:43,630
+فهيتحول اللون للون Red
+
+373
+00:27:50,080 --> 00:27:55,620
+هي الصورة في الميديا فيها نيتريات ضفت reagent a وb
+
+374
+00:27:55,620 --> 00:28:00,540
+ماتغير اللون فساعت وقتها هضيف ال zinc powder بعد
+
+375
+00:28:00,540 --> 00:28:04,180
+إضافة ال zinc powder إذا اتحول اللون للون red هذا
+
+376
+00:28:04,180 --> 00:28:12,060
+معناه إنه النتيجة negative ضفت reagent هاي نفس
+
+377
+00:28:12,060 --> 00:28:16,340
+الفكرة ماتغير اللون فساعتها هضيف reagent c reagent
+
+378
+00:28:16,340 --> 00:28:22,980
+c إذا بعد إضافة reagent cإذا اتحول اللون للون red
+
+379
+00:28:22,980 --> 00:28:27,720
+يبقى هاي معناه انه البكتيريا ما عندها الانزيم هاي
+
+380
+00:28:27,720 --> 00:28:32,980
+النتيجة ال negative هاي النتيجة ال برضه ملخص ضفت
+
+381
+00:28:32,980 --> 00:28:39,800
+زراعة بكتيريا في ال media in ال nitrate ضفت
+
+382
+00:28:39,800 --> 00:28:43,880
+reagent a و reagent b بقولي لو ضفت reagent a و b و
+
+383
+00:28:43,880 --> 00:28:47,760
+اتحول اللون للون أحمر معناه هاي النتيجة positive
+
+384
+00:28:48,610 --> 00:28:52,390
+لكن إذا ما تغير اللون هضيف ال zinc powder إذا بعد
+
+385
+00:28:52,390 --> 00:28:56,070
+إضافة ال zinc powder ما تغير اللون هذا معناه إنه
+
+386
+00:28:56,070 --> 00:28:59,410
+عملت complete reduction أو اتحول ال nitrate ل
+
+387
+00:28:59,410 --> 00:29:03,790
+ammonia يبقى high في حال كانت positive لكن الصورة
+
+388
+00:29:03,790 --> 00:29:11,210
+الأبل في حال كان negativeهي نفس الفكرة بقوللي انه
+
+389
+00:29:11,210 --> 00:29:18,170
+طبعا بعد ما اضفت ال reagent A وB هانضفت اول شئ
+
+390
+00:29:18,170 --> 00:29:21,690
+طبعا زراعتي البكتيريا ضفت reagent A وB ما تغير
+
+391
+00:29:21,690 --> 00:29:25,590
+اللون هانتغير اللون للون Red يبقى هاي نتيجة
+
+392
+00:29:25,590 --> 00:29:29,070
+positive هاني نتيجة ما بنحكي negative بنضيف ال
+
+393
+00:29:29,070 --> 00:29:32,970
+zinc powder فدول التيوبين ضفت ال zinc powder على
+
+394
+00:29:32,970 --> 00:29:36,590
+ال tube هاي اللي طلعت colorless بعد إضافة reagent
+
+395
+00:29:36,590 --> 00:29:41,910
+A وBلحظنا إنه ما تغير اللون فبالتالي هذا معناه إنه
+
+396
+00:29:41,910 --> 00:29:44,590
+البكتيريا عملت complete reduction بما إنه عملت
+
+397
+00:29:44,590 --> 00:29:48,130
+complete reduction يبقى هي positive للإنزيم لو
+
+398
+00:29:48,130 --> 00:29:51,630
+اتحول اللون للون أحمر قلنا إنه هذه النتيجة معناها
+
+399
+00:29:51,630 --> 00:29:55,810
+negative ما عندها الإنزيم لكن تحول اللون الأحمر
+
+400
+00:29:55,810 --> 00:30:00,310
+نتيجة إنه الـ Zinc powder عمل reducing للـ Nitrate
+
+401
+00:30:00,310 --> 00:30:03,610
+اللي موجود في ال media لم يصل له أي استهلاك اتحول
+
+402
+00:30:03,610 --> 00:30:07,770
+ل Nitrateأتفعل مع agent A و B فعطاني اللون الأحمر
+
+403
+00:30:07,770 --> 00:30:13,750
+هاي نفس الفكرة هاي ال media مش مزروعة ك control
+
+404
+00:30:13,750 --> 00:30:18,770
+هان بدون قبل ما أضيف اللي هو ال zinc powder اتحول
+
+405
+00:30:18,770 --> 00:30:22,490
+اللون للون red يبقى هي positive هان ضفت ال zinc
+
+406
+00:30:22,490 --> 00:30:25,350
+powder بعد إضافة ال zinc powder كانت colorless
+
+407
+00:30:25,350 --> 00:30:29,070
+يبقى هي positive بعد ما ضفت ال zinc powder اتحول
+
+408
+00:30:29,070 --> 00:30:34,200
+اللون للون red يبقى هي negativeبقول لي في تلاتة
+
+409
+00:30:34,200 --> 00:30:39,320
+أمثلة على البكتيريا بالنسبة لفحص النيترات
+
+410
+00:30:39,320 --> 00:30:43,320
+reduction test كيف بدي أنا أفرق بينهم قلنا الـ
+
+411
+00:30:43,320 --> 00:30:46,380
+Staphylococcus epidermidis ما عندها القدرة أنها
+
+412
+00:30:46,380 --> 00:30:50,600
+تستخدم النيترات as terminal electron acceptor
+
+413
+00:30:50,600 --> 00:30:54,780
+فبالتالي مش هتقدر تختزل النيترات لنيترا تبقى هي
+
+414
+00:30:54,780 --> 00:30:56,980
+negative لـ Staphylococcus epidermidis negative
+
+415
+00:30:56,980 --> 00:31:01,350
+للفحصالـ E.coli وكل عقلة الـ Enterobacteriasi
+
+416
+00:31:01,350 --> 00:31:06,330
+بتختزل للنيترات فقط لـ Nitrite فبالتالي بعد إضافة
+
+417
+00:31:06,330 --> 00:31:10,650
+Reagent A وB كل عقلة الـ Enterobacteriasi هيتحول
+
+418
+00:31:10,650 --> 00:31:15,590
+لونها لون ال media للون أحمر الصدومونس قلنا إنه هي
+
+419
+00:31:15,590 --> 00:31:18,350
+تعتبر بتعمل Complete Reduction فبالتالي بعد ما
+
+420
+00:31:18,350 --> 00:31:22,750
+أضيف Reagent A وB هيصير عندي مش هيصير تفاعل هيكون
+
+421
+00:31:22,750 --> 00:31:25,730
+colorless هذا لا يعني إنه نتيجة negative عشان هيك
+
+422
+00:31:25,730 --> 00:31:29,060
+بضيف ال zinc powderإذا ضلت colorless معناه إنه
+
+423
+00:31:29,060 --> 00:31:32,080
+عملت complete reduction الـ pseudomonas
+
+424
+00:31:32,080 --> 00:31:36,980
+fluorescence قلنا إن عندها القدرة إنها تفرز
+
+425
+00:31:36,980 --> 00:31:40,860
+pigment diffusable yellow green pigment بتفرز
+
+426
+00:31:40,860 --> 00:31:45,180
+pigment diffusable قلنا soluble بتقدر إنها اتلون
+
+427
+00:31:45,180 --> 00:31:49,810
+الكوني وكمان الطبقممكن ان انا اشوفها fluorescence
+
+428
+00:31:49,810 --> 00:31:54,910
+in the ultraviolet light في ال UV بتعمل reduce
+
+429
+00:31:54,910 --> 00:32:01,070
+للنيتريات completely هتعمل completely reduction
+
+430
+00:32:01,070 --> 00:32:04,750
+complete reduction للنيتريات ل molecular nitrogen
+
+431
+00:32:04,750 --> 00:32:09,390
+عشان هيك لما اضيف reagent A و B مش هيتحول اللون
+
+432
+00:32:09,390 --> 00:32:13,900
+للون أحمر في حال كان البكتيريا pseudomonasهنا
+
+433
+00:32:13,900 --> 00:32:17,480
+بقولي دي في بعض ال safety rules عند استخدام
+
+434
+00:32:17,480 --> 00:32:21,680
+reagent اللي هو فحص النيترات reaction test قولي
+
+435
+00:32:21,680 --> 00:32:25,760
+reagent بيه اللي هو ال diet مثل نفط القمعين يعتبر
+
+436
+00:32:25,760 --> 00:32:30,570
+carcinogenic عشان هيكطبعا بتلبسي ال gloves بتحاولي
+
+437
+00:32:30,570 --> 00:32:36,650
+أنك تبعدي عن جلدك هذه المواد و كمان انك تستنشق هذه
+
+438
+00:32:36,650 --> 00:32:40,470
+المواد كمان ال reagent is sulfonylic acid يعتبر
+
+439
+00:32:40,470 --> 00:32:46,990
+كوزاتك مواد حارقة طبعا avoid skin contact بحاول
+
+440
+00:32:46,990 --> 00:32:50,730
+أني أبعده عن الجلد و عن إيدي و ما أني أنا أستنشقه
+
+441
+00:32:51,070 --> 00:32:55,950
+كمان أيضا عند استخدام الزنك باودر بيقوللي ممنوع
+
+442
+00:32:55,950 --> 00:33:00,410
+انك تستنشقيها او انه تلمس جلدكممنوع تستخدمي الـ
+
+443
+00:33:00,410 --> 00:33:03,210
+mouth by beating في فحص الـ Nitrate Reduction Test
+
+444
+00:33:03,210 --> 00:33:08,210
+لأن كل ال reagent اللي بنستخدمها تعتبر طبعا خطيرة،
+
+445
+00:33:08,210 --> 00:33:11,990
+هيك من جون خلصنا الفحص ال Nitrate Reduction Test
+
+446
+00:33:11,990 --> 00:33:17,930
+وخلصنا شعب ترب التلات فحوصات ال Ureas و ال Endol و
+
+447
+00:33:17,930 --> 00:33:21,410
+ال Nitrate Reduction Test السلام عليكم و رحمة الله
+
+448
+00:33:21,410 --> 00:33:22,210
+وبركاته
+
diff --git a/PL9fwy3NUQKwZWgAb_V1ATlRRsH-6i3cPC/WtgYOo5DhhI.srt b/PL9fwy3NUQKwZWgAb_V1ATlRRsH-6i3cPC/WtgYOo5DhhI.srt
new file mode 100644
index 0000000000000000000000000000000000000000..6703d8e1855842538449f68f4009e5f77fdd35c6
--- /dev/null
+++ b/PL9fwy3NUQKwZWgAb_V1ATlRRsH-6i3cPC/WtgYOo5DhhI.srt
@@ -0,0 +1,2350 @@
+1
+00:00:02,060 --> 00:00:05,800
+السلام عليكم ورحمة الله وبركاته يعطيكم العافية
+
+2
+00:00:05,800 --> 00:00:09,280
+جميعًا اليوم المحاضرة عن Selective and
+
+3
+00:00:09,280 --> 00:00:13,560
+Differential Media في المحاضرة أو في المعامل
+
+4
+00:00:13,560 --> 00:00:17,540
+السابقة، اتكلمنا عن الـ Media، عرفنا الـ Media
+
+5
+00:00:17,540 --> 00:00:22,180
+وصنفنا الـ Media أو قسمنا الـ Media حسب الـ
+
+6
+00:00:22,180 --> 00:00:25,800
+Function، حسب الـ Consistency، حسب الـ Nutrient
+
+7
+00:00:25,800 --> 00:00:31,000
+وذكرنا عدة تصنيفات تحت كل صنف من هذه الأصناف
+
+8
+00:00:31,570 --> 00:00:36,270
+بالنسبة للـ function، قلنا ممكن أن تكون general
+
+9
+00:00:36,270 --> 00:00:40,210
+media، ممكن أن تكون transport media، ممكن أن
+
+10
+00:00:40,210 --> 00:00:43,030
+تكون selective media، ممكن أن تكون differential
+
+11
+00:00:43,030 --> 00:00:48,090
+media. اليوم سنتحدث عن ال selective و ال
+
+12
+00:00:48,090 --> 00:00:52,610
+differential media. سنشرح شو يعني selective، شو يعني
+
+13
+00:00:52,610 --> 00:00:55,610
+differential، وأمثلة على selective media و
+
+14
+00:00:55,610 --> 00:01:00,390
+differential media. طيب، بالأول خلينا نتكلم، شو يعني
+
+15
+00:01:00,390 --> 00:01:04,740
+selective. Selective بالعربي معناها انتقائي أو
+
+16
+00:01:04,740 --> 00:01:10,780
+اختياري. بدنا نختار ونمي نوع معين من الميكروبات
+
+17
+00:01:10,780 --> 00:01:14,800
+يبقى هي selection for the growth of certain
+
+18
+00:01:14,800 --> 00:01:19,160
+microorganisms. بدنا نمي نوع معين من الميكروبات
+
+19
+00:01:19,160 --> 00:01:25,360
+differential يعني تميزي. بدي أميز كل نوع من هاي
+
+20
+00:01:25,360 --> 00:01:30,340
+الميكروبات اللي نمت بلون معين. يبقى differential to
+
+21
+00:01:30,340 --> 00:01:34,720
+distinguish every group or species. يبقى ال
+
+22
+00:01:34,720 --> 00:01:38,620
+selective راح ينمي الـ group من الـ microorganisms
+
+23
+00:01:38,620 --> 00:01:43,660
+راح يجي الـ differential يعطي كل species في هذا الـ
+
+24
+00:01:43,660 --> 00:01:49,000
+group إشي مميز عن الثاني. هذا بالنسبة لمعنى
+
+25
+00:01:49,000 --> 00:01:53,080
+selective و differential media. ممكن الميديا تكون
+
+26
+00:01:53,080 --> 00:01:57,920
+selective لحال، ممكن تكون الميديا differential لحال،
+
+27
+00:01:57,920 --> 00:02:02,100
+ممكن تكون الميديا selective و differential بنفس
+
+28
+00:02:02,100 --> 00:02:05,680
+الوقت، يعني أن تنمي جروب من الـ microorganisms
+
+29
+00:02:05,680 --> 00:02:11,180
+بعدين الميديا نفسها راح تتميز، تعمل differentiate،
+
+30
+00:02:11,180 --> 00:02:14,700
+تعطي كل الـ species في هذا الجروب إشي مميز عن
+
+31
+00:02:14,700 --> 00:02:19,080
+الثاني. طيب،
+
+32
+00:02:19,440 --> 00:02:22,320
+in addition to general purpose media which
+
+33
+00:02:22,320 --> 00:02:25,040
+allow the growth of most types of bacteria,
+
+34
+00:02:25,040 --> 00:02:29,120
+microbiologists use specialized media to identify
+
+35
+00:02:29,120 --> 00:02:34,660
+or isolate specific groups of bacteria. شو الهدف من
+
+36
+00:02:34,660 --> 00:02:38,920
+الـ media؟ الهدف من الـ media أني أنمي معظم
+
+37
+00:02:38,920 --> 00:02:42,320
+الميكروبات، allow the growth of most types of
+
+38
+00:02:42,320 --> 00:02:47,660
+bacteria. بدي أنا أنمي معظم الميكروبات، لكن بقول لي
+
+39
+00:02:47,660 --> 00:02:54,770
+أن الـ Microbiologists بيختار الـ media أو بيختار
+
+40
+00:02:54,770 --> 00:02:59,750
+media خاصة عشان يعزل أنواع معينة من الميكروبات
+
+41
+00:02:59,750 --> 00:03:06,110
+ويعمل تعريف لها. إحنا قلنا أو شرحنا قبل كده أن
+
+42
+00:03:06,110 --> 00:03:10,490
+الـ microbiologists بيختار الـ media حسب نوع العينة.
+
+43
+00:03:10,490 --> 00:03:14,630
+بكون أنا عارفة أن والله مثلاً عينة الـ urine، الـ most
+
+44
+00:03:14,630 --> 00:03:19,920
+common pathogens in urine X وY وZ، فبالتالي أنا
+
+45
+00:03:19,920 --> 00:03:25,440
+بختار media تنمي هدول البكتيريا، الـ most common
+
+46
+00:03:25,440 --> 00:03:30,520
+pathogens in urine، عشان أعزل هذه الأنواع المعينة من
+
+47
+00:03:30,520 --> 00:03:34,220
+الميكروبات وأعمل لها تعريف. selective and
+
+48
+00:03:34,220 --> 00:03:38,620
+differential media are used to isolate or identify
+
+49
+00:03:38,620 --> 00:03:42,400
+particular organisms. أكيد الـ selective و الـ
+
+50
+00:03:42,400 --> 00:03:46,390
+differential media تستخدم لعزل نوع معين من
+
+51
+00:03:46,390 --> 00:03:51,430
+الميكروبات وتعريفها. selective media، هنعرف شو هي
+
+52
+00:03:51,430 --> 00:03:55,030
+الـ selective media، وأنا ذكرت التعريف في بداية
+
+53
+00:03:55,030 --> 00:04:00,070
+المعمل. allow certain types of organisms to grow. بدها
+
+54
+00:04:00,070 --> 00:04:04,990
+تسمح لنوع معين فقط أن ينمو، and inhibit the growth
+
+55
+00:04:04,990 --> 00:04:09,950
+of other organisms. وباقي الـ organisms هتعمل
+
+56
+00:04:09,950 --> 00:04:14,270
+inhibition، مش هتعمله kill، هتعمل inhibition لـ
+
+57
+00:04:14,270 --> 00:04:18,750
+growth of other organisms. طيب، بقول الـ selectivity،
+
+58
+00:04:18,750 --> 00:04:24,090
+الـ selective agent، إيش هو؟ for example, organisms
+
+59
+00:04:24,090 --> 00:04:27,910
+that can utilize a given sugar are easily selected
+
+60
+00:04:27,910 --> 00:04:31,050
+by making that sugar the only carbon source in
+
+61
+00:04:31,050 --> 00:04:35,750
+the medium. على سبيل المثال، ذكرنا الـ media، مرت معنا
+
+62
+00:04:35,750 --> 00:04:39,770
+اللي هي الـ Simon-Citrate Agar. قلنا هذه الـ media
+
+63
+00:04:39,770 --> 00:04:45,130
+تعتبر selective media. ليش selective media؟ لأن فقط
+
+64
+00:04:45,130 --> 00:04:48,790
+الـ organisms اللي عندها القدرة على استهلاك الـ
+
+65
+00:04:48,790 --> 00:04:54,230
+citrate كمصدر للـ carbon فقط هو اللي هينمو، أو فقط
+
+66
+00:04:54,230 --> 00:04:58,410
+هي البكتيريا اللي هتنمو، لأن الـ citrate هو المصدر
+
+67
+00:04:58,410 --> 00:05:03,950
+الوحيد للـ carbon في هذه الـ media. عشان هيك يعتبر
+
+68
+00:05:03,950 --> 00:05:08,990
+الـ sodium citrate في media السمن سيتريت أجار هو الـ
+
+69
+00:05:08,990 --> 00:05:14,550
+selective agent. هو اللي خلى أن فقط نوع معين
+
+70
+00:05:14,550 --> 00:05:17,290
+من البكتيريا اللي عندها القدرة على استهلاك الـ citrate
+
+71
+00:05:17,290 --> 00:05:21,130
+هي اللي تنمو، أما الـ other organisms مش هيقدر ينمو،
+
+72
+00:05:21,130 --> 00:05:24,870
+هيعمل inhibition، أو مش هيقدر ينمو في الأصل كمان.
+
+73
+00:05:24,870 --> 00:05:29,860
+تمام؟ مثال على selective media، أو وضّح فكرة شو يعني
+
+74
+00:05:29,860 --> 00:05:33,240
+selective media، شو أنا بضيف في هذه الـ media عشان
+
+75
+00:05:33,240 --> 00:05:40,300
+تصير selective media. on
+
+76
+00:05:40,300 --> 00:05:43,560
+the other hand, selective inhibition of some types
+
+77
+00:05:43,560 --> 00:05:47,900
+of microorganisms can be achieved by adding dyes,
+
+78
+00:05:47,900 --> 00:05:52,440
+antibiotics, salts, or specific inhibitors which affect
+
+79
+00:05:52,440 --> 00:05:55,740
+the metabolism or enzyme systems of the organisms.
+
+80
+00:05:56,450 --> 00:06:06,550
+بقول لي الـ selective agent ممكن يكون salt، ممكن
+
+81
+00:06:06,550 --> 00:06:11,150
+يكون dyes، ممكن يكون صبغات. الـ selective agent ممكن
+
+82
+00:06:11,150 --> 00:06:19,390
+يكون dyes، ممكن يكون antibiotic، ممكن يكون salt، all
+
+83
+00:06:19,390 --> 00:06:22,530
+specific inhibitors. بتأثر على الـ metabolism و الـ
+
+84
+00:06:22,530 --> 00:06:28,060
+enzyme للـ organisms. هدول هم اللي هيخلوا أو هيعملوا
+
+85
+00:06:28,060 --> 00:06:31,700
+inhibition لـ الـ other organisms. الـ salts، و الـ
+
+86
+00:06:31,700 --> 00:06:36,680
+antibiotics، و الـ salts. فبالتالي الـ other organisms هي
+
+87
+00:06:36,680 --> 00:06:41,440
+اللي هتنمو فقط. يبقى هذا يعتبر selective inhibition.
+
+88
+00:06:41,440 --> 00:06:47,320
+بيعمل inhibition لـ organism معين، فبالتالي مش هيقدر
+
+89
+00:06:47,320 --> 00:06:52,260
+ينمو، فقط الـ other organisms هو اللي هينمو. ذا، كل
+
+90
+00:06:52,260 --> 00:06:57,670
+أمثلة. على سبيل المثال، مجموعة تحتوي على بوتاسيوم
+
+91
+00:06:57,670 --> 00:07:04,010
+توليرايت، أو سوديوم أزايد، أو ثاليوم أسيتات. هدول
+
+92
+00:07:04,010 --> 00:07:09,950
+طبعاً بتركيز واحد من عشرة إلى خمسة من عشرة، ينهيبط
+
+93
+00:07:09,950 --> 00:07:15,490
+أكثر من جرام بكتيريا سالبة. تمام. هدول هم الـ
+
+94
+00:07:15,490 --> 00:07:19,290
+potassium tellurite، و Sodium Azide، و Thallous
+
+95
+00:07:19,290 --> 00:07:24,270
+Acetate. هدول كلهم يعتبروا كـ selective agents.
+
+96
+00:07:24,270 --> 00:07:30,010
+بيعملوا inhibition لجرام سالبة bacteria. يبقى
+
+97
+00:07:30,010 --> 00:07:34,050
+هيكونوا، لو أنا media ضفت عليها هدول التلاتة الـ
+
+98
+00:07:34,050 --> 00:07:38,500
+salts. طبعاً هدول يعتبروا salts. من الأمثلة على الـ
+
+99
+00:07:38,500 --> 00:07:41,400
+Selective Inhibition. بيعملوا Inhibition لـ Growth
+
+100
+00:07:41,400 --> 00:07:44,940
+of Gram Negative Bacteria. يبقى هيكونوا Selection
+
+101
+00:07:44,940 --> 00:07:49,240
+لمين؟ للجرام موجبة Bacteria. هيكونوا Selective
+
+102
+00:08:19,630 --> 00:08:26,070
+هدول طبعاً الـ salts أي
+
+103
+00:08:26,070 --> 00:08:30,630
+media بتحتوي على هدول الـ salts، هاي الأملاح بتعمل هاي
+
+104
+00:08:30,630 --> 00:08:33,970
+الأملاح inhibition لجرام سالبة bacteria. بالتالي
+
+105
+00:08:33,970 --> 00:08:37,770
+هتسمح بنمو جرام موجبة bacteria. selection of
+
+106
+00:08:37,770 --> 00:08:43,230
+جرام موجبة bacteria. طيب، media supplemented with
+
+107
+00:08:43,230 --> 00:08:46,970
+penicillin. الـ penicillin يعتبر antibiotic. هاي مثال
+
+108
+00:08:46,970 --> 00:08:49,510
+إحنا قلنا ممكن يكون dyes، ممكن يكون antibiotic،
+
+109
+00:08:49,510 --> 00:08:53,160
+ممكن يكون salt. أمثلة على الـ Salt هيها التلاتة هذول
+
+110
+00:08:53,160 --> 00:08:56,960
+يعتبروا Salt. قلنا بتعمل Inhibition لـ Growth of
+
+111
+00:08:56,960 --> 00:09:00,100
+Gram Negative Bacteria. طيب، الميديا اللي فيها
+
+112
+00:09:00,100 --> 00:09:03,520
+Penicillin. الـ Penicillin يعتبر Antibiotic. هي مثال
+
+113
+00:09:03,520 --> 00:09:07,900
+على الـ Antibiotic. أو الـ crystal violet. الـ crystal
+
+114
+00:09:07,900 --> 00:09:13,240
+violet يعتبر Dye. استعينى. يبقى هيك ذكرنا كل، ذكرنا
+
+115
+00:09:13,240 --> 00:09:16,920
+مثال على كل نوع: Dye، Antibiotic أو Salt. قلنا الـ
+
+116
+00:09:16,920 --> 00:09:20,580
+Penicillin و الـ crystal violet بيعملوا inhibition
+
+117
+00:09:20,580 --> 00:09:25,380
+للجرام موجبة bacteria. يبقى هي هتكون then
+
+118
+00:09:25,380 --> 00:09:28,220
+selective لمين؟ للجرام سالبة bacteria.
+
+119
+00:09:47,230 --> 00:09:51,410
+يبقى أي media فيها بنيسيلان و crystal violet هتنمي
+
+120
+00:09:51,410 --> 00:09:56,050
+فقط لجرام سالبة bacteria. selective of Gram
+
+121
+00:09:56,050 --> 00:09:59,610
+سالبة bacteria. هتعمل inhibition للجرام موجبة
+
+122
+00:09:59,610 --> 00:10:06,510
+bacteria. والبنيسيلان إحنا قلنا في بداية المعامل أنه
+
+123
+00:10:06,510 --> 00:10:11,330
+الاستهداف لهي الروابط الـ cross-link ما بين
+
+124
+00:10:11,330 --> 00:10:14,510
+الـ peptidoglycans في الـ gram positive bacteria
+
+125
+00:10:14,510 --> 00:10:17,110
+فبالتالي إحنا قلنا أنه هيأثر على الـ gram positive
+
+126
+00:10:17,110 --> 00:10:20,390
+bacteria، ومش هيأثر على الـ gram negative bacteria.
+
+127
+00:10:20,390 --> 00:10:26,740
+هذا كان الـ action تبعه للبنيسيلين. طيب، هنذكر أن
+
+128
+00:10:26,740 --> 00:10:30,040
+media، نفس الكلام اللي فوق. tellurite agar، إيش يعني
+
+129
+00:10:30,040 --> 00:10:33,940
+tellurite agar؟ يعني media فيها بوتاسيوم توليرايت.
+
+130
+00:10:33,940 --> 00:10:37,240
+قلنا هي media بتعمل inhibition للجرام سالبة
+
+131
+00:10:37,240 --> 00:10:41,480
+bacteria. يبقى هي selective للجرام موجبة bacteria.
+
+132
+00:10:41,480 --> 00:10:45,320
+is used to select for Gram positive organisms. هي
+
+133
+00:10:45,320 --> 00:10:50,260
+كاتبة هي media بتعمل، هي بتختار، بتعمل اختيار للجرام
+
+134
+00:10:50,260 --> 00:10:53,500
+موجبة bacteria. اتفقنا فوق هذا الكلام، على هذا
+
+135
+00:10:53,500 --> 00:10:57,370
+الكلام. بما أنها بتعمل inhibition لجرام سالبة
+
+136
+00:10:57,370 --> 00:11:01,930
+bacteria، يبقى هي select for gram positive bacteria.
+
+137
+00:11:01,930 --> 00:11:05,630
+And nutrient agar supplemented with penicillin. لو
+
+138
+00:11:05,630 --> 00:11:10,290
+nutrient agar ضفت عليها penicillin، إحنا قلنا الـ
+
+139
+00:11:10,290 --> 00:11:13,370
+penicillin بيعمل inhibition للجرام موجبة bacteria،
+
+140
+00:11:13,370 --> 00:11:18,050
+يبقى هو select for gram negative organisms. هيك
+
+141
+00:11:18,050 --> 00:11:25,190
+بيكون المعلومات واضحة. يعني الكلام هذا تقريباً ذاكر
+
+142
+00:11:25,190 --> 00:11:27,930
+"هان" إنها بتعمل "inhibition"، و "هان" ذاكر إنها
+
+143
+00:11:27,930 --> 00:11:32,430
+بتكون "selective" لمين؟ يبقى أنا بحفظ الـ "salt" هذا
+
+144
+00:11:32,430 --> 00:11:37,590
+بيعمل "inhibition" لمين؟ يبقى بشكل بديهي بكون عارفة
+
+145
+00:11:37,590 --> 00:11:48,850
+"selective" لأي نوع من البكتيريا. طيب،
+
+146
+00:11:49,150 --> 00:11:52,290
+ذكرنا شو يعني selective media، نيجي لـ
+
+147
+00:11:52,290 --> 00:11:56,110
+differential media. differential media doesn't
+
+148
+00:11:56,110 --> 00:12:00,250
+necessarily inhibit bacterial growth, but instead
+
+149
+00:12:00,250 --> 00:12:03,430
+makes the bacteria look different. بقول لي هي مش
+
+150
+00:12:03,430 --> 00:12:07,870
+بالضرورة أن تعمل inhibition للـ growth للبكتيريا،
+
+151
+00:12:07,870 --> 00:12:13,830
+لكن بتعمل تمييز لكل كولوني، لكل species أو كل group،
+
+152
+00:12:13,830 --> 00:12:18,130
+بتخليها أو بتعطيها شكل مميز. كل group أو كل species
+
+153
+00:12:18,600 --> 00:12:22,440
+أو every colony. حتى differential media work best
+
+154
+00:12:22,440 --> 00:12:26,640
+with closely related organisms, and the differential
+
+155
+00:12:26,640 --> 00:12:32,100
+agent is what causes the bacteria to look different.
+
+156
+00:12:32,100 --> 00:12:35,200
+بقول لي الـ differential agent زي ما قلنا فيه
+
+157
+00:12:35,200 --> 00:12:37,880
+selective agent، زي الـ salt، زي الـ dye، زي الـ
+
+158
+00:12:37,880 --> 00:12:42,420
+antibiotic. برضه في عندي differential agent، مادة هي
+
+159
+00:12:42,420 --> 00:12:47,350
+اللي بتخلي البكتيريا مختلفة. owing the presence of
+
+160
+00:12:47,350 --> 00:12:50,190
+certain dyes or chemicals in the medium, the organisms
+
+161
+00:12:50,190 --> 00:12:53,670
+will produce characteristic changes or a growth
+
+162
+00:12:53,670 --> 00:12:56,610
+pattern that are used for identification or
+
+163
+00:12:56,610 --> 00:13:00,410
+differentiation. بقول لي كمان أن هذا الـ
+
+164
+00:13:00,410 --> 00:13:04,690
+differential agent ممكن يكون وجوده، هو عبارة عن وجود
+
+165
+00:13:04,690 --> 00:13:08,050
+presence of certain dyes or chemicals موجودة في هذه
+
+166
+00:13:08,050 --> 00:13:11,550
+الـ media. ممكن يكون dyes، ممكن يكون chemicals موجود
+
+167
+00:13:11,550 --> 00:13:16,560
+في هذه الـ media. بيخلي الـ organisms يعمل نمو أو تغير
+
+168
+00:13:16,560 --> 00:13:21,420
+في النمو أو في نمط النمو، وهذا هيساعد في الـ
+
+169
+00:13:21,420 --> 00:13:25,840
+identification و الـ differentiation. هنفهم أكتر لما
+
+170
+00:13:25,840 --> 00:13:28,900
+نذكر أمثلة على selective و differential media،
+
+171
+00:13:28,900 --> 00:13:31,800
+نتعرف مين هو الـ selective agent، كيف هذا الـ
+
+172
+00:13:31,800 --> 00:13:35,380
+selective agent بيميز كل كولوني أو كل species أو
+
+173
+00:13:35,380 --> 00:13:39,860
+group في هذه الـ media. A variety of selective and
+
+174
+00:13:39,860 --> 00:13:42,840
+differential media are used in medical diagnostic
+
+175
+00:13:43,450 --> 00:13:47,110
+and water pollution laboratories, and in food and
+
+176
+00:13:47,110 --> 00:13:51,530
+dairy laboratories. بقول لي الـ Selective و الـ
+
+177
+00:13:51,530 --> 00:13:56,830
+Differential media بتستخدم في التشخيص الطبي، زي ما
+
+178
+00:13:56,830 --> 00:14:02,130
+إحنا كخصائي تحاليل طبية نستخدم الـ Differential و
+
+179
+00:14:02,130 --> 00:14:05,290
+الـ Selective media عشان أعمل identification
+
+180
+00:14:05,290 --> 00:14:11,510
+لـ microorganisms الموجودة عندي، عشان أعرف شو المسبب
+
+181
+00:14:11,510 --> 00:14:18,240
+للالتهاب. كمان يستخدم في تلوث المياه، بيفلتره طبعاً
+
+182
+00:14:18,240 --> 00:14:22,640
+المياه عشان يشوفوا الـ coliforms، بعدين بيحطوها على
+
+183
+00:14:22,640 --> 00:14:29,220
+طبق الـ EMB. طبعاً طبق الـ EMB ه
+
+223
+00:17:29,450 --> 00:17:31,990
+الـ Selective agent اللي هو التركيز العالي من
+
+224
+00:17:31,990 --> 00:17:36,950
+الملح بالنسبة لـ Differential agent هذه الـ media
+
+225
+00:17:36,950 --> 00:17:41,830
+تعتبر Selective and Differential agent. من هو الـ
+
+226
+00:17:41,830 --> 00:17:45,230
+Differential agent؟ الـ Differential agent في هذه
+
+227
+00:17:45,230 --> 00:17:50,390
+الـ media المانيتول. المانيتول يعتبر sugar. طيب
+
+228
+00:17:50,600 --> 00:17:53,660
+البكتيريا التي نمت (Staphylococcus) في هذه الـ media
+
+229
+00:17:53,660 --> 00:17:57,860
+بقول لي: (Staphylococcus) بدي أميزها، بدي أميز الـ
+
+230
+00:17:57,860 --> 00:18:01,600
+species للاستافيلوكوكاي. Differentiate Staphylococcus
+
+231
+00:18:01,600 --> 00:18:05,260
+كوكاي الـ species. يبقى الـ differential agent هو
+
+232
+00:18:05,260 --> 00:18:10,700
+المانيتول sugar، عشان نعرف كيف تعمل differentiate
+
+233
+00:18:10,700 --> 00:18:15,340
+بين الاستافيلوكوكاي الـ species. بنعرف أن في هذه
+
+234
+00:18:15,340 --> 00:18:20,770
+الـ media BH Indicator، اللي هو الفينول Red، الفينول non
+
+235
+00:18:20,770 --> 00:18:24,250
+-red. مر معنا للمرة الرابعة. أول مرة في اليوريا،
+
+236
+00:18:24,250 --> 00:18:27,270
+المرة الثانية فيه أو أول مرة في الـ fermentation،
+
+237
+00:18:27,270 --> 00:18:30,630
+ثانية مرة في اليوريا، ثالث مرة في الـ triple sugar
+
+238
+00:18:30,630 --> 00:18:34,830
+iron agar، وهي في الـ manitol salt agar. حدوده في الـ
+
+239
+00:18:34,830 --> 00:18:38,130
+acid yellow. في الـ base بيكون non-red. بقول لي
+
+240
+00:18:38,130 --> 00:18:42,510
+البكتيريا التي عندها الاستافيلوكوكاي، طبعاً هي فقط
+
+241
+00:18:42,510 --> 00:18:45,670
+التي ستتحمل الملوحة العالية، فقط التي ستنمو على الـ
+
+242
+00:18:45,670 --> 00:18:50,110
+manitol salt agar. بنميز الاستافيلوكوكاي الـ
+
+243
+00:18:50,110 --> 00:18:53,230
+species. بقول لي: في من الاستافيلوكوكاي عندها
+
+244
+00:18:53,230 --> 00:18:57,210
+القدرة أن تعمل fermentation للمانيتول، وفي non
+
+245
+00:18:57,210 --> 00:19:00,550
+manitol fermenter. طيب البكتيريا التي عندها القدرة
+
+246
+00:19:00,550 --> 00:19:05,350
+أن تعمل fermentation للمانيتول، ستعمل ferment
+
+247
+00:19:05,350 --> 00:19:08,270
+للمانيتول. طبعاً المانيتول sugar، ستصله fermentation،
+
+248
+00:19:09,320 --> 00:19:12,860
+ستعمل fermentation، ستعمل fermentation للمانيتول،
+
+249
+00:19:12,860 --> 00:19:17,500
+نواتج عملية الـ fermentation acid ستعمل على خفض الـ
+
+250
+00:19:17,500 --> 00:19:23,680
+pH. سيتحول لون الوسط للون yellow. يبقى colonies
+
+251
+00:19:23,680 --> 00:19:28,740
+surrounded by yellow zone. هي بتكون معناها أنه
+
+252
+00:19:28,740 --> 00:19:32,160
+manitol fermenter. من الأمثلة على الـ manitol
+
+253
+00:19:32,160 --> 00:19:36,510
+fermenter:  الاستافيلوكوكس أوريس. طب لو البكتيريا
+
+254
+00:19:36,510 --> 00:19:39,730
+الاستافيلوكوكاي الـ species ما عندها القدرة أن تعمل
+
+255
+00:19:39,730 --> 00:19:45,610
+fermentation للمانيتول، مش هيتم إنتاج أي تغيير في
+
+256
+00:19:45,610 --> 00:19:50,690
+اللون. فبالتالي ستظل الـ colony لونها colorless، و
+
+257
+00:19:50,690 --> 00:19:54,510
+لون الطبق لونه pink. من الأمثلة عليها: الاستافيلو
+
+258
+00:19:54,510 --> 00:19:58,950
+كوكاس إيبيدرميس. يبقى لو مسكنا طبق manitol salt agar،
+
+259
+00:19:58,950 --> 00:20:03,510
+ولقينا بكتيريا نامية، يبقى بعرف أنه ستافيلوكوكاي، لأن
+
+260
+00:20:03,510 --> 00:20:08,270
+هذه الـ media مختارة (Selective) للاستافيلوكوكاي فقط، التي
+
+261
+00:20:08,270 --> 00:20:13,010
+ستنمو. لستافيلوكوكاي. طيب أعطتني لون أصفر، يبقى
+
+262
+00:20:13,010 --> 00:20:17,750
+بعرف أنه عملت Manitol Fermenter. شو يعني Manitol
+
+263
+00:20:17,750 --> 00:20:20,690
+Fermenter؟ يعني عملت fermentation للمانيتول. اتكون
+
+264
+00:20:20,690 --> 00:20:25,510
+أسيد عمل على خفض الـ pH. فبالتالي تحول لون الوسط
+
+265
+00:20:25,510 --> 00:20:29,730
+للون أصفر. يبقى لون الوسط، لون الـ media لونها زهري،
+
+266
+00:20:29,730 --> 00:20:34,210
+سأجد أن الـ media تحول لونها للون الأصفر، وممكن
+
+267
+00:20:34,210 --> 00:20:37,170
+إذا تكونت كمية كبيرة من الأسيد، أجد الـ colony
+
+268
+00:20:37,170 --> 00:20:40,650
+نفسها تحولت للون الأصفر. من الأمثلة عليها
+
+269
+00:20:40,650 --> 00:20:44,830
+الاستافيلوكوكا أوريس. طيب لو مسكت الطبق ولقيت
+
+270
+00:20:44,830 --> 00:20:48,390
+والله عندي كولوني لونها colorless، لكن الطبق
+
+271
+00:20:48,390 --> 00:20:51,590
+حولها لونه pink، يبقى بعرف أن البكتيريا
+
+272
+00:20:51,590 --> 00:20:54,630
+ستافيلوكوكاي، لأن الـ media selection of
+
+273
+00:20:54,630 --> 00:20:59,080
+ستافيلوكوكاي. طيب لونها colorless، ولون الـ media
+
+274
+00:20:59,080 --> 00:21:02,900
+لا يزال لونه pink، يبقى بعرف أنه هي non manitol
+
+275
+00:21:02,900 --> 00:21:07,400
+fermenter. ما عملت fermentation للمنيتول، ما تغير لون
+
+276
+00:21:07,400 --> 00:21:12,500
+الـ media. لا يزال لون الـ media pink، ولا يزال لون الكولوني
+
+277
+00:21:12,500 --> 00:21:16,580
+colorless. من الأمثلة عليها: ستافيلوكوكس إيبيدرميس.
+
+278
+00:21:16,580 --> 00:21:22,920
+هذا بالنسبة لمنيتول salt agar. Manitol salt agar
+
+279
+00:21:22,920 --> 00:21:25,580
+is a selective media. هي selective و differential
+
+280
+00:21:25,580 --> 00:21:29,960
+media. لكن هو هان بده يشرح يعني selective media
+
+281
+00:21:29,960 --> 00:21:33,540
+used for isolation of pathogenic Staphylococci.
+
+282
+00:21:33,540 --> 00:21:37,680
+تستخدم لعزل الـ Staphylococci، لأنها فقط تنمي الـ
+
+283
+00:21:37,680 --> 00:21:41,080
+Staphylococci. الـ media تحتوي على Manitol كـ
+
+284
+00:21:41,080 --> 00:21:44,380
+differential agent. في non-red indicator كبيئة
+
+285
+00:21:44,380 --> 00:21:49,560
+Indicator. 7.5% Sodium chloride كـ selective agent.
+
+286
+00:21:49,970 --> 00:21:53,470
+Ferment Manitol هو الذي سيجعله Differentiate
+
+287
+00:21:53,470 --> 00:21:55,830
+هو الـ Differentiation اللي فيه Nordred هو الـ BH
+
+288
+00:21:55,830 --> 00:21:58,770
+Indicator. قلنا الـ Selective، الـ High Salt
+
+289
+00:21:58,770 --> 00:22:01,970
+Concentration، التركيز العالي من الملح، سيجعل
+
+290
+00:22:01,970 --> 00:22:05,510
+Inhibition لمعظم البكتيريا الأخرى بخلاف
+
+291
+00:22:05,510 --> 00:22:09,550
+Staphylococci. معادل Staphylococci. بقول لي: التركيز
+
+292
+00:22:09,550 --> 00:22:12,450
+العالي من الملح لن يعمل ذلك لـ Gram Negative
+
+293
+00:22:12,450 --> 00:22:16,470
+بكتيريا. سيجعل Inhibition. اتفقنا أنه ما بيصير كلّه
+
+294
+00:22:16,470 --> 00:22:22,900
+يصير Inhibition، بيصير Inhibition لنمو الكائنات الحية الأخرى على
+
+295
+00:22:22,900 --> 00:22:27,280
+Manitol Salt Agar. Pathogenic Staphylococcus aureus
+
+296
+00:22:27,280 --> 00:22:30,740
+طبعاً الـ Pathogenic من الـ Staphylococcus هي الـ Staphylococcus
+
+297
+00:22:30,740 --> 00:22:34,480
+أوريس. produce small colonies surrounded by yellow
+
+298
+00:22:34,480 --> 00:22:39,400
+zone. اتفقنا، بتفرج طبعاً هي بتكون small colony محيطة
+
+299
+00:22:39,400 --> 00:22:43,680
+باللون الأصفر نتيجة fermentation للـ Manitol. السبب
+
+300
+00:22:43,680 --> 00:22:46,800
+في تغيير اللون هذا: Staphylococcus aureus هي ferment
+
+301
+00:22:46,800 --> 00:22:50,540
+the manitol producing an acid which is turned
+
+302
+00:22:50,540 --> 00:22:53,260
+trans the indicator from red to yellow. هذا هو
+
+303
+00:22:53,260 --> 00:22:58,160
+السبب الذي يجعل Staphylococcus aureus أن الكولوني
+
+304
+00:22:58,160 --> 00:23:01,320
+حولها لون أصفر نتيجة الـ fermentation للمانيتول.
+
+305
+00:23:01,320 --> 00:23:03,880
+تكون acid عمل على خفض الـ pH. مش تحول الـ
+
+306
+00:23:03,880 --> 00:23:08,060
+indicator من red لـ yellow، لكن الآخر Staphylococcus ما
+
+307
+00:23:08,060 --> 00:23:12,650
+تعمل fermentation للمانيتول، مش هيتم إنتاج أي تغيير
+
+308
+00:23:12,650 --> 00:23:17,490
+في لون الـ media اللي هو أصلاً pink. تمام. بيقول: الـ
+
+309
+00:23:17,490 --> 00:23:20,230
+growth of other types of bacteria is generally
+
+310
+00:23:20,230 --> 00:23:24,450
+inhibited. الكائنات الحية الأخرى غير الاستافيلوكوكاي
+
+311
+00:23:24,450 --> 00:23:29,430
+يصير لها inhibition وليس killed. طيب نجي لكل نوع
+
+312
+00:23:29,430 --> 00:23:32,090
+من أنواع البكتيريا: الاستافيلوكوكاس أوريس على
+
+313
+00:23:32,090 --> 00:23:37,080
+المانيتول سولت أجار. أكيد هي Salt Tolerant، ستتحمل
+
+314
+00:23:37,080 --> 00:23:39,720
+الملوحة العالية، فبالتالي ستنمو. أي بكتيريا
+
+315
+00:23:39,720 --> 00:23:45,620
+Staphylococci طبعاً ستنمو لأنها ستتحمل درجة الملوحة
+
+316
+00:23:45,620 --> 00:23:51,420
+العالية. تمام. بالنسبة لـ Staphylococcus aureus، ماذا
+
+317
+00:23:51,420 --> 00:23:54,840
+ستفعل؟ ستعمل fermentation للمنيتول، فسيحول لون الـ
+
+318
+00:23:54,840 --> 00:23:58,160
+media للون yellow. Staphylococcus epidermidis برضه
+
+319
+00:23:58,160 --> 00:24:01,400
+ستتحمل الملوحة العالية، بما أنها Staphylococci، لكن
+
+320
+00:24:01,400 --> 00:24:04,620
+لن تعمل fermentation للمانيتول، فسيبقى لون الـ
+
+321
+00:24:04,620 --> 00:24:08,900
+media لونه pink. الـ Micrococcus luteus not salt
+
+322
+00:24:08,900 --> 00:24:12,680
+tolerant، doesn't growth on manitol. نمسح هذه
+
+323
+00:24:12,680 --> 00:24:17,680
+الجملة.
+
+324
+00:24:17,680 --> 00:24:21,600
+Micrococcus. بقول لي: نفس الاستافيلوكوكاس تنمو
+
+325
+00:24:21,600 --> 00:24:25,540
+على الـ manitol salt agar، وبتعمل fermentation
+
+326
+00:24:25,540 --> 00:24:29,760
+للمانيتول. طيب احنا قلنا أنه فقط الـ manitol salt
+
+327
+00:24:29,760 --> 00:24:34,230
+agar هي التي تنمو على ميديا الـ Manitol salt
+
+328
+00:24:34,230 --> 00:24:38,490
+agar. كيف نحكي أن الـ Micrococcus نفس Staphylococcus
+
+329
+00:24:38,490 --> 00:24:43,670
+aureus، تنمو وتعطي اللون الأصفر؟ بدنا نعرف معلومة:
+
+330
+00:24:43,670 --> 00:24:47,530
+never ever trust in selectivity of the
+
+331
+00:24:47,530 --> 00:24:51,070
+selective media. ما بدي أثق في الـ selective media،
+
+332
+00:24:51,070 --> 00:24:54,370
+لازم أنا أعمل فحوصات، أعمل جرام ستين، أعمل فحوصات
+
+333
+00:24:54,370 --> 00:24:58,650
+كيميائية. هي بتسهل عليّ التعريف، لكن أنا ما أضمن حد ما
+
+334
+00:24:58,650 --> 00:25:01,630
+أفتح طبق الـ Manitol Salt Agar، أشوف لون أصفر،
+
+335
+00:25:01,630 --> 00:25:05,350
+أخلص على طول Staphylococcus Aureus؟ لا! لازم أعمل
+
+336
+00:25:05,350 --> 00:25:09,990
+جرام ستين، لازم أمشي فحوصات، لازم أمشي كل الفحوصات،
+
+337
+00:25:09,990 --> 00:25:13,690
+حتى أوصل لتعريفي النهائي. فقط بعرف أنه هي عملت
+
+338
+00:25:13,690 --> 00:25:17,410
+fermentation للـ Manitol من ميديا الاستافيلو، من
+
+339
+00:25:17,410 --> 00:25:21,250
+ميديا الـ Manitol Salt Agar. يبقى هنا فيه طبعاً
+
+340
+00:25:21,250 --> 00:25:24,850
+دراسات حديثة بتأكد أن الـ Micrococcus نفس الـ
+
+341
+00:25:24,850 --> 00:25:28,320
+Staphylococcus Aureus، على الـ Manitol Salt Agar
+
+342
+00:25:28,320 --> 00:25:33,080
+تنمو وتعطي اللون الأصفر. فبالتالي بدنا نستفيد من
+
+343
+00:25:33,080 --> 00:25:36,100
+هذه المعلومة: «never ever trust in selectivity
+
+344
+00:25:36,100 --> 00:25:41,440
+of selective media».
+
+345
+00:25:41,440 --> 00:25:46,960
+طيب بالنسبة للصور، هذه الصور ممكن أن تأتي في
+
+346
+00:25:46,960 --> 00:25:49,820
+الامتحان. هذا طبق Manitol Salt Agar. الـ media لون
+
+347
+00:25:49,820 --> 00:25:52,760
+الـ media هو لون زهري، نفس اللون الموجود هنا، الذي
+
+348
+00:25:52,760 --> 00:25:58,370
+أنا أشير إليه. زراعة بكتيريا، بكتيريا أعطتني حولت
+
+349
+00:25:58,370 --> 00:26:02,610
+اللون، حول الكولوني لون أصفر، وحتى لون الكولوني لون
+
+350
+00:26:02,610 --> 00:26:07,910
+أصفر. لكن هنا ألاحظ أنه ظلّ اللون pink. يبقى high
+
+351
+00:26:07,910 --> 00:26:12,270
+بما أنها نمت، أحكي أنها ستافيلوكوكاي. طيب، أو نحكي
+
+352
+00:26:12,270 --> 00:26:16,950
+سؤال: tolerant. تحمّلت الملوحة العالية. طيب، نجي لـ
+
+353
+00:26:20,090 --> 00:26:23,110
+الـ fermentation. بما أنه تحوّل اللون للون أصفر،
+
+354
+00:26:23,110 --> 00:26:26,390
+يبقى أحكي Manitol Fermenter. لكن هنا أحكي not
+
+355
+00:26:26,390 --> 00:26:31,010
+Manitol... non Manitol Fermenter. يبقى هذا احتمال
+
+356
+00:26:31,010 --> 00:26:34,950
+أنها تكون. من الأمثلة عليها: ستافيلوكوكس أوريس، التي
+
+357
+00:26:34,950 --> 00:26:38,770
+تعطي هذا اللون، وهي ستافيلوكوكس إيبيدرميس. هنا
+
+358
+00:26:38,770 --> 00:26:41,470
+نفس الشيء، هنا ألاحظ اللون أصفر، وهنا اللون أحمر،
+
+359
+00:26:41,470 --> 00:26:44,910
+يبقى هذه Manitol Fermenter، هذه Non Manitol
+
+360
+00:26:44,910 --> 00:26:50,160
+Fermenter. هنا نلاحظ أن الطبق والكولوني صار لونها
+
+361
+00:26:50,160 --> 00:26:55,160
+أصفر. يبقى هي، بما أنها نمت، يبقى هي salt tolerant. من
+
+362
+00:26:55,160 --> 00:26:58,000
+الأمثلة عليها: الاستافيلوكوكاي. طيب أعطت اللون
+
+363
+00:26:58,000 --> 00:27:01,800
+الأصفر، يبقى manitol fermenter. من الأمثلة عليها
+
+364
+00:27:01,800 --> 00:27:07,290
+الاستافيلوكوكاس أوريس. نمت. يبقى البكتيريا salt tolerant
+
+365
+00:27:07,290 --> 00:27:12,790
+ما أعطت اللون الأصفر، ذا لون الـ media لونه pink، و
+
+366
+00:27:12,790 --> 00:27:16,350
+الـ colony كانت colorless، يبقى هي non-manitol
+
+367
+00:27:16,350 --> 00:27:18,890
+fermenter. من الأمثلة عليها: الاستافيلوكوكس
+
+368
+00:27:18,890 --> 00:27:22,890
+إيبيدرميس. هذه الـ media manitol salt agar، لكن
+
+369
+00:27:22,890 --> 00:27:27,230
+ما لاحظت أي نمو، يبقى هي أصلاً ما تحمّلت الملوحة
+
+370
+00:27:27,230 --> 00:27:30,590
+العالية، ما كان في عندي نمو، يبقى هذا احتمال أنها
+
+371
+00:27:30,590 --> 00:27:34,980
+تكون أي بكتيريا ما عدا الاستافيلوكوكاي. بشكل عام،
+
+372
+00:27:34,980 --> 00:27:37,640
+بنمحي الـ Micrococcus، قلنا لأنها تنمو على
+
+373
+00:27:37,640 --> 00:27:41,960
+المانيتول سولت أجار. ممكن نكتب اللي هي أي ميديا
+
+374
+00:27:41,960 --> 00:27:47,560
+جرام موجبة كوكاي. مثلاً الـ Clostridium، الباسيلس،
+
+375
+00:27:47,560 --> 00:27:52,380
+الستربتوكوكاي، أي بكتيريا جرام موجبة أو جرام
+
+376
+00:27:52,380 --> 00:27:56,380
+جرام موجبة، طبعاً من غير الاستافيلوكوكاي أو
+
+377
+00:27:56,380 --> 00:27:59,620
+جرام سالبة بكتيريا. هذه لو أنا نميتها على
+
+378
+00:27:59,620 --> 00:28:03,560
+المانيتول سولت أجار، لن تنمو، لأنها لن تتحمل درجة
+
+379
+00:28:03,560 --> 00:28:07,380
+الملوحة العالية. يبقى لو جئنا في الامتحان سؤال أنه
+
+380
+00:28:07,380 --> 00:28:12,000
+طبق manitol salt agar، لكن ما لاحظنا فيه نمو، نحكي
+
+381
+00:28:12,000 --> 00:28:17,660
+no growth because البكتيريا ما قدرة تتحمل، non-salt
+
+382
+00:28:17,660 --> 00:28:21,020
+tolerant، ما قدرة تتحمل الملوحة العالية. يبقى هي أي
+
+383
+00:28:21,020 --> 00:28:24,660
+بكتيريا غير الاستافيلوكوكاي، وغير الميكروكوكاس.
+
+384
+00:28:24,660 --> 00:28:28,580
+أذكري أي مثال، مثلاً gram negative bacteria،
+
+385
+00:28:28,580 --> 00:28:34,080
+Pseudomonas، E.coli، Klebsiella، Streptococci. أي مثال من
+
+386
+00:28:34,080 --> 00:28:38,460
+ما عدا الاستافيلوكوكاي والميكروكوكاس. هكذا نكون
+
+387
+00:28:38,460 --> 00:28:45,020
+خلصنا أول ميديا، اللي هي الـ Manitol salt agar،
+
+388
+00:28:45,020 --> 00:28:47,960
+selective و differential media. تعرفنا شو الـ
+
+389
+00:28:47,960 --> 00:28:51,700
+selective agent، وشو الـ differential agent. طيب نجي
+
+390
+00:28:51,700 --> 00:28:56,800
+لثاني ميديا: using methylene blue agar. هذه الـ media
+
+391
+00:28:56,800 --> 00:29:01,560
+برضه تعتبر selective و differential media. تمام. طيب
+
+392
+00:29:01,560 --> 00:29:04,140
+شو الـ selective agent في هذه الـ media؟ الـ
+
+393
+00:29:04,140 --> 00:29:08,000
+selective agent هو الإيوزين والميثيلين بلو. هذول
+
+394
+00:29:08,000 --> 00:29:11,560
+يُعتبروا dyes. قلنا الـ selective agent ممكن يكون
+
+395
+00:29:11,560 --> 00:29:15,400
+dyes. أم
+
+445
+00:32:28,340 --> 00:32:31,340
+بتكون أسد، بقولي هذا الأسد بيرتبط بالإيوزين
+
+446
+00:32:31,340 --> 00:32:34,560
+والميثيلين بلو، لكن الرابط ما بين الإيوزين
+
+447
+00:32:34,560 --> 00:32:39,400
+والميثيلين بلو بتكون رابط ما بينهم، فبالتالي
+
+448
+00:32:39,400 --> 00:32:45,260
+بيعطيني اللون الأخضر، الـ metallic green، الـ Coliform
+
+449
+00:32:45,260 --> 00:32:47,960
+بكتيريا، إحنا قلنا E.coli, Klebsiella, Citrobacter,
+
+450
+00:32:48,080 --> 00:32:51,760
+Enterobacter، الـ E.coli عرفنا أنه شيء خاص في هاي
+
+451
+00:32:51,760 --> 00:32:54,100
+من عائلة الـ Lactose Fermenter، بتعطي Metallic
+
+452
+00:32:54,100 --> 00:32:57,120
+Green Sheen، وعرفنا شو السبب، طيب الـ Klebsiella
+
+453
+00:32:57,120 --> 00:32:59,160
+Citrobacter، الـ Enterobacter بقول بتعمل
+
+454
+00:32:59,160 --> 00:33:02,660
+fermentation للـ Lactose، بتكون أسد زي الـ E.coli
+
+455
+00:33:02,660 --> 00:33:07,620
+بتربط مع الإيوزين والميثيلين بلو، بتعطي لون Pink to
+
+456
+00:33:07,620 --> 00:33:13,260
+Purple Colony أو Dark Purple Colony، لكن الـ E.coli
+
+457
+00:33:13,260 --> 00:33:16,820
+بتكون رابطة ما بين الإيوزين والميثيلين بلو، لكن هنا
+
+458
+00:33:16,820 --> 00:33:20,720
+ما بتتكون رابطة، فبنتج اللون البربل، هنا بتتكون رابطة
+
+459
+00:33:20,720 --> 00:33:23,540
+ما بين الإيوزين والميثيلين بلو، فبنتج اللون الـ
+
+460
+00:33:23,540 --> 00:33:26,940
+Metallic Green Sheen، هذا بالنسبة للـ Lactose
+
+461
+00:33:26,940 --> 00:33:29,860
+Fermenter، الـ Non-Lactose Fermenter بقول لي ما
+
+462
+00:33:29,860 --> 00:33:32,560
+بيصير أي fermentation للـ Lactose، فبالتالي مش
+
+463
+00:33:32,560 --> 00:33:36,300
+هيتغير لون الـ media، ومش هتتغير لون الكولوني،
+
+464
+00:33:36,300 --> 00:33:41,410
+هتظلها colorless، أو ممكن أنها تاخد لون الكولوني،
+
+465
+00:33:41,410 --> 00:33:45,270
+لون الـ media، طبعاً ممكن أن تكون colorless، أو ممكن
+
+466
+00:33:45,270 --> 00:33:48,250
+تاخد لون الـ media، لون الـ media لون violet فاتح،
+
+467
+00:33:48,250 --> 00:33:52,250
+فأبدأ أفرق ما بين الـ lactose و الـ non lactose، خاصة
+
+468
+00:33:52,250 --> 00:33:55,610
+أنه الـ lactose في الـ fermenter بتكون dark purple، لكن هاي
+
+469
+00:33:55,610 --> 00:34:00,070
+لو أخدت لون الـ media، بيكون لون violet فاتح، طيب لو
+
+470
+00:34:00,070 --> 00:34:04,450
+طبق AMP، لكن ما لاقيت فيه أي نمو، يبقى بعرف أنه هي
+
+471
+00:34:04,450 --> 00:34:07,830
+high gram positive bacteria، صار لها inhibition
+
+472
+00:34:08,070 --> 00:34:12,570
+بواسطة الصبغات، الأيوزين والمثيلين بلو، قبل هيك اتعرفنا
+
+473
+00:34:12,570 --> 00:34:24,190
+أو اتعرفنا على الـ EMB، نيجي للـ slides، EMB is a
+
+474
+00:34:24,190 --> 00:34:28,830
+primary differential media، هي تعتبر differential
+
+475
+00:34:28,830 --> 00:34:32,390
+media، وعرفنا لأن فيها الـ lactose كـ differential
+
+476
+00:34:32,390 --> 00:34:36,580
+agent، however it does inhibit the growth of some of
+
+477
+00:34:36,580 --> 00:34:39,700
+the gram positive bacteria، و اللي بتعمل inhibition
+
+478
+00:34:39,700 --> 00:34:43,920
+لـ some of the gram positive bacteria، تمام، فبالتالي
+
+479
+00:34:43,920 --> 00:34:48,560
+هي selective for the gram negative bacteria، EMB is
+
+480
+00:34:48,560 --> 00:34:51,380
+used to differentiate between enteric lactose
+
+481
+00:34:51,380 --> 00:34:54,320
+fermenter، الكوليفورم، and non-lactose fermenter، هي
+
+482
+00:34:54,320 --> 00:34:56,660
+بتفرق بين الـ lactose fermenter و non-lactose
+
+483
+00:34:56,660 --> 00:34:59,200
+fermenter من عائلة الـ enterobacteriaceae، اللي هي
+
+484
+00:34:59,200 --> 00:35:02,330
+الكوليفورم و الـ non-coliform، بالإضافة بتعمل
+
+485
+00:35:02,330 --> 00:35:05,850
+identification للـ E.coli، لأن الـ E.coli بتعطيها
+
+486
+00:35:05,850 --> 00:35:10,350
+لون مميز، لـ Metallic Green Sheen، فبالتالي بتساعد
+
+487
+00:35:10,350 --> 00:35:14,830
+في الـ identification للـ E.coli، فده بشكل خاص، Eosin
+
+488
+00:35:14,830 --> 00:35:17,550
+Methylene Blue Agar، بقولي هذه الـ media، الـ EMB
+
+489
+00:35:17,550 --> 00:35:22,850
+اللي هي Eosin Methylene Blue Agar، contains peptone،
+
+490
+00:35:22,850 --> 00:35:27,380
+تحتوي على، تحتوي على اللاكتوز، طبعاً البيبتون كمصدر
+
+491
+00:35:27,380 --> 00:35:30,980
+للتغذية، أي ميديا فيها بيبتون كمصدر للتغذية عشان
+
+492
+00:35:30,980 --> 00:35:35,160
+البكتيريا تنمو وتتكاثر، اللاكتوز differential agent،
+
+493
+00:35:35,160 --> 00:35:39,140
+الداي، الأيوزين والميثيلين بلو تعتبر selective
+
+494
+00:35:39,140 --> 00:35:44,080
+agent، واللي الـ sugar provide، هذا الـ sugar هو الـ
+
+495
+00:35:44,080 --> 00:35:49,400
+substrate، عشان البكتيريا تعملي fermentation، The
+
+496
+00:35:49,400 --> 00:35:51,960
+dyes inhibit the growth of gram positive
+
+497
+00:35:51,960 --> 00:35:54,880
+bacteria organisms، قلنا إن الصبغات، الميثيلين بلو
+
+498
+00:35:54,880 --> 00:35:57,360
+والإيوزين بتعمل inhibition لـ gram positive
+
+499
+00:35:57,360 --> 00:36:02,000
+organisms، and under acidic conditions also produce
+
+500
+00:36:02,000 --> 00:36:08,200
+dark purple، بقولي إذا تكون عندي acidic product،
+
+501
+00:36:08,200 --> 00:36:13,700
+يبقى هاي هترتبط، طبعاً المركب هيرتبط بالإيوزين
+
+502
+00:36:13,700 --> 00:36:18,880
+والميثيلين بلو، فبالتالي هتعطيني لون dark purple،
+
+503
+00:36:18,880 --> 00:36:22,320
+يبقى الـ dyes بنفس الوقت، مع الـ under acidic
+
+504
+00:36:22,320 --> 00:36:24,900
+conditions، مع الـ acidic conditions، لو تكون عندي
+
+505
+00:36:24,900 --> 00:36:29,400
+acidic product، هترتبط مع، اللي هي هذا الـ acidic
+
+506
+00:36:29,400 --> 00:36:33,740
+product، هيرتبط مع الصبغات، هيعطيني لون dark purple،
+
+507
+00:36:33,740 --> 00:36:37,740
+فبالتالي يعتبر الـ dyes برضه as pH indicator، كـ
+
+508
+00:36:37,740 --> 00:36:42,220
+selective agent، طيب
+
+509
+00:36:44,060 --> 00:36:48,060
+الأيوزين والميثيلين بلو، تجعل lactose fermenter تحصل على
+
+510
+00:36:48,060 --> 00:36:51,220
+لون pink colony، بقولي أن الأيوزين والميثيلين بلو
+
+511
+00:36:51,220 --> 00:36:55,200
+تجعل lactose fermenter تحصل على لون pink colony، أو هي
+
+512
+00:36:55,200 --> 00:36:59,480
+بالضبط بتاعتي dark purple، ليش بتاعتي الـ dark purple؟
+
+513
+00:36:59,480 --> 00:37:02,380
+قلنا بتكون أسد، الـ product، هذا الأسد الـ product
+
+514
+00:37:02,380 --> 00:37:06,620
+بيرتبط مع هذه الصبغات، فبيعطيني الـ pink or dark purple،
+
+515
+00:37:06,620 --> 00:37:11,940
+الـ E.coli قلنا إنه إلها شيء خاص، تمام، بقولي هذه
+
+516
+00:37:11,940 --> 00:37:15,300
+الصبغة بتترسب في الخلايا، وبتعطي اللون الـ
+
+517
+00:37:15,300 --> 00:37:19,080
+metallic green sheen، يبقى الـ E.coli بتعطي الـ
+
+518
+00:37:19,080 --> 00:37:24,240
+metallic green sheen، تمام، لكن الـ other coliform غير
+
+519
+00:37:24,240 --> 00:37:28,080
+الـ E.coli بتعطي اللون الـ dark purple، وعرفنا ليش
+
+520
+00:37:28,080 --> 00:37:30,800
+بتعطي اللون الـ metallic green sheen، لأنه بتكون
+
+521
+00:37:30,800 --> 00:37:33,960
+رابط ما بين الإيوزين والميثيلين blue، طب الـ non
+
+522
+00:37:33,960 --> 00:37:36,740
+-lactose fermenter بقولي هتضلها colorless، لأنه ما
+
+523
+00:37:36,740 --> 00:37:39,500
+هيكون fermentation، أو ممكن تاخد لون الـ media اللي
+
+524
+00:37:39,500 --> 00:37:43,740
+هو اللون الـ violet الفاتح، زي السالمونيلا، بقولي
+
+525
+00:37:43,740 --> 00:37:47,560
+السالمونيلا تعتبر non-lactose fermenter، لو نميتها
+
+526
+00:37:47,560 --> 00:37:50,660
+على الـ EMB، ممكن تكون colorless، وممكن تاخد لون الـ
+
+527
+00:37:50,660 --> 00:37:54,800
+media، طبعاً هي واحدة من أهم المسببات في الـ food
+
+528
+00:37:54,800 --> 00:37:57,120
+poisoning، في التسمم الغذائي،
+
+529
+00:37:59,590 --> 00:38:03,050
+طيب بالنسبة لهاي الـ media، used to confirm the
+
+530
+00:38:03,050 --> 00:38:05,670
+presence of E.coli in water samples contaminated
+
+531
+00:38:05,670 --> 00:38:09,830
+with sewage، أو فيه contamination، ولهاي الـ media بشكل
+
+532
+00:38:09,830 --> 00:38:12,550
+خاص، مش كمان بتفرق بين الـ lactose و الـ non-lactose،
+
+533
+00:38:12,550 --> 00:38:15,510
+لأنه هنتعرف على media، كمان بتميز بين الـ lactose
+
+534
+00:38:15,510 --> 00:38:19,250
+و الـ non-lactose، وفعلاً بتعطي ألوان واضحة، لكن هاي
+
+535
+00:38:19,250 --> 00:38:23,630
+الـ media بشكل خاص، بتستخدم عشان أؤكد وجود الـ E.coli
+
+536
+00:38:23,630 --> 00:38:28,190
+في عينات الـ water samples contaminated with sewage،
+
+537
+00:38:29,020 --> 00:38:33,600
+ليش هاي بتستخدم؟ لأن الـ E.coli قلنا في هذه الـ media
+
+538
+00:38:33,600 --> 00:38:37,140
+بتعطي لون مميز، بتعطي لون metallic green sheen،
+
+539
+00:38:37,140 --> 00:38:42,680
+فبالتالي هي بتأكد وجود الـ E.coli، you will use this
+
+540
+00:38:42,680 --> 00:38:46,380
+agar again within water sampling experiments to
+
+541
+00:38:46,380 --> 00:38:49,440
+differentiate between E.coli and Enterobacter،
+
+542
+00:38:49,440 --> 00:38:53,580
+بقولي في هذه الـ media، بميز بين الـ E.coli و الـ
+
+543
+00:38:53,580 --> 00:38:58,740
+Enterobacter على الـ EMB، طيب ليش بتميز في هذه
+
+544
+00:38:58,740 --> 00:39:03,100
+الميديا بين الـ E.coli و الـ Enterobacter؟ الـ E
+
+545
+00:39:03,100 --> 00:39:06,280
+.coli و الـ Enterobacter يعتبروا lactose fermenter، لكن الـ E
+
+546
+00:39:06,280 --> 00:39:10,000
+.coli قلنا بينتج acid، بيرتبط مع الـ eosin و
+
+547
+00:39:10,000 --> 00:39:13,660
+الميثيلين blue، بتكون رابطة بين الـ eosin و
+
+548
+00:39:13,660 --> 00:39:16,900
+الميثيلين blue، فبيعطيني اللون الـ metallic green
+
+549
+00:39:16,900 --> 00:39:22,500
+sheen، بالنسبة للـ Enterobacter، هذه اسمها fish eye،
+
+550
+00:39:22,500 --> 00:39:27,020
+الـ Enterobacter، ملاحظ أن شكلها زي الـ fish eye،
+
+551
+00:39:27,810 --> 00:39:32,610
+عارفين بتعمل fermentation للـ lactose، تمام، بتعمل
+
+552
+00:39:32,610 --> 00:39:36,470
+fermentation للـ lactose، sorry، بتعمل fermentation
+
+553
+00:39:36,470 --> 00:39:42,310
+للـ lactose، بتكون acid، هذا الـ acid بيرتسب مع الصبغة،
+
+554
+00:39:42,310 --> 00:39:48,010
+تمام، بيترسب في المنتصف، الصبغة بتترسب في المنتصف،
+
+555
+00:39:48,520 --> 00:39:52,120
+على الطرف بيكون في عندي colorless، فبالتالي بلاحظ أنه
+
+556
+00:39:52,120 --> 00:39:56,200
+هي زي عين السمكة، زي عين السمكة، يبقى السمكة
+
+557
+00:39:56,200 --> 00:40:00,080
+بتترسب في منتصف الـ colony، على الأطراف بحيث أن الـ
+
+558
+00:40:00,080 --> 00:40:04,720
+colony colorless، يبقى بتكون زي عين السمكة، فش… فش
+
+559
+00:40:04,720 --> 00:40:09,120
+eye، فبالتالي هيك الفرق بين الـ fish eye و الـ
+
+560
+00:40:09,120 --> 00:40:15,570
+interior، بأكتر، هذا بالنسبة للميديا
+
+561
+00:40:15,570 --> 00:40:17,310
+اللي بستخدمها بالتفريق بين الـ E.coli و الـ
+
+562
+00:40:17,310 --> 00:40:20,090
+Enterobacter، لأن الـ E.coli بتدي metallic green sheen،
+
+563
+00:40:20,090 --> 00:40:23,810
+وعرفنا شو السبب، الـ Enterobacter بتعطي fish eye،
+
+564
+00:40:23,810 --> 00:40:27,550
+لأنه بتعمل fermentation للـ Lactose، بيرتبط مع
+
+565
+00:40:27,550 --> 00:40:32,350
+الصبغة، بتترسب الصبغة في النص، على الطرف بحيث أنه في
+
+566
+00:40:32,350 --> 00:40:36,010
+عندي colorless، فبالتالي بتكون زي عين السمكة، هيك
+
+567
+00:40:36,010 --> 00:40:37,550
+بنكون خلصنا، نيجي لـ
+
+568
+00:40:45,100 --> 00:40:50,340
+لسلايدات، للصور، هنا ملاحظ طبق A و B، نمت عليه
+
+569
+00:40:50,340 --> 00:40:54,160
+البكتيريا، يبقى هي gram negative bacteria، تمام، ظهر
+
+570
+00:40:54,160 --> 00:40:56,880
+اللون الأخضر، يبقى بحكي أنه والله هي احتمال أنها
+
+571
+00:40:56,880 --> 00:41:01,020
+تكون E.coli، وعرفنا ليش تكون اللون الأخضر، بالنسبة
+
+572
+00:41:01,020 --> 00:41:07,140
+لهنا، fish eye colony، ملاحظ أن الصبغة اترسبت في
+
+573
+00:41:07,140 --> 00:41:09,660
+المنتصف، وعلى الطرف في عندي colorless، يبقى هي
+
+574
+00:41:09,660 --> 00:41:13,580
+احتمال أنها تكون Enterobacter، السبب أنه صار في
+
+575
+00:41:13,580 --> 00:41:17,400
+عندي fermentation لللاكتوز، تكون acid، ارتبط مع
+
+576
+00:41:17,400 --> 00:41:22,480
+الصبغة، ارتبط مع الصبغة، فبالتالي كون لون dark
+
+577
+00:41:22,480 --> 00:41:26,100
+purple، اترسبت الصبغة في المنتصف، وعلى الطرف كان في
+
+578
+00:41:26,100 --> 00:41:30,010
+عندي colorless، يبقى هي Enterobacter، طبعاً باقي اللي
+
+579
+00:41:30,010 --> 00:41:33,950
+هي الكليبسيلة والستروبكتر بتعطي لون dark زي هذا،
+
+580
+00:41:33,950 --> 00:41:38,070
+لكن ما بتترسب في المنتصف، الكليبسيلة والستروبكتر
+
+581
+00:41:38,070 --> 00:41:43,230
+بروتيوس وسالمونيلا، نفس الشيء، بروتيوس وسالمونيلا non
+
+582
+00:41:43,230 --> 00:41:46,730
+-lactose fermenter، فبالتالي مش هيتغير لون
+
+583
+00:41:46,730 --> 00:41:51,270
+الكولوني، هتظل لونها colorless، أو ممكن أنها تاخد
+
+584
+00:41:51,270 --> 00:41:54,030
+لون الـ media، اللي هو اللون، اللي هو الـ violet
+
+585
+00:41:54,030 --> 00:41:58,530
+الفاتح، بالنسبة لـ Staphylococcus aureus، لـ
+
+586
+00:41:58,530 --> 00:42:02,510
+Staphylococcus aureus على طبق الـ EMB، المفترض ما
+
+587
+00:42:02,510 --> 00:42:05,050
+إنها تنمو، لأنه هي gram positive bacteria، يبقى لو
+
+588
+00:42:05,050 --> 00:42:08,830
+جبنا في الامتحان EMB، ولاحظنا أنه والله في عندي طبق
+
+589
+00:42:08,830 --> 00:42:13,650
+ما في عندي أي نمو على الطبق، يبقى بحكي إنه هاي الـ
+
+590
+00:42:13,650 --> 00:42:17,670
+bacteria، صار لها inhibition، بواسطة اللي هي
+
+591
+00:42:17,670 --> 00:42:20,690
+selective agent، الإيوزين والميثيلين بلو، يبقى
+
+592
+00:42:20,690 --> 00:42:24,570
+احتمال إنها تكون gram positive bacteria، هذا بالنسبة
+
+593
+00:42:24,570 --> 00:42:31,150
+لـ… اللي هو الـ… شو تفسير الألوان؟ طبعاً الـ
+
+594
+00:42:31,150 --> 00:42:35,010
+EMB، الـ lactose fermenter لون dark purple، لكن الـ
+
+595
+00:42:35,010 --> 00:42:38,530
+E.coli فقط، الـ E.coli بتعطي metallic green sheen،
+
+596
+00:42:38,530 --> 00:42:42,030
+الـ Enterobacter بتميزها الـ fish eye colony، الـ
+
+597
+00:42:42,030 --> 00:42:44,610
+non lactose fermenter بتضلها colorless، أو ممكن
+
+598
+00:42:44,610 --> 00:42:49,410
+تاخد لون الـ media، لون الـ… الوسط، أي gram
+
+599
+00:42:49,410 --> 00:42:52,540
+positive bacteria مش هتنمو على هذا الوسط، الـ E.coli
+
+600
+00:42:52,540 --> 00:42:56,040
+من الملاحظ أنه والله هنا هي lactose fermenter،
+
+601
+00:42:56,040 --> 00:43:01,580
+وعرفنا شو سبب تكون اللون الأخضر على الـ EMB لـ E
+
+602
+00:43:01,580 --> 00:43:07,840
+.coli، هنا الـ Enterobacter باخد اللون الـ pink، تمام،
+
+603
+00:43:07,840 --> 00:43:13,660
+على الـ EMB، إحنا قلنا pink or dark purple، يبقى هنا
+
+604
+00:43:13,660 --> 00:43:18,050
+من الملاحظ أنه إحنا مش هنشوف، طبعاً الـ fish eye colony،
+
+605
+00:43:18,050 --> 00:43:22,390
+Enterobacter، المفترض أنها تكون fish eye colony، ليش
+
+606
+00:43:22,390 --> 00:43:26,070
+مش هنشوف هاي الـ fish eye colony؟ لأنه هاي مش
+
+607
+00:43:26,070 --> 00:43:29,470
+isolated colony، ما زرعت بطريقة الأربع عشان أحصل
+
+608
+00:43:29,470 --> 00:43:32,990
+على isolated colony، فبالتالي مش هشوف الـ fish eye
+
+609
+00:43:32,990 --> 00:43:35,390
+colony، عشان أشوف الـ fish eye colony لازم أزرع
+
+610
+00:43:35,390 --> 00:43:41,590
+بطريقة الأربع، طيب هيك خلصنا الـ EMB، نيجي لآخر media
+
+611
+00:43:41,590 --> 00:43:46,120
+اللي هي الـ MacConkey agar، الـ MacConkey زي الـ EMB،
+
+612
+00:43:46,120 --> 00:43:48,860
+selective لـ gram negative bacteria، يبقى لـ
+
+613
+00:43:48,860 --> 00:43:52,180
+gram positive bacteria هيصير لها inhibition، مين
+
+614
+00:43:52,180 --> 00:43:55,460
+الـ selective agent؟ الـ selective agent نفس
+
+615
+00:43:55,460 --> 00:44:00,400
+هذه الميديا، الـ EMB برضه يعتبر dyes، لكن بيختلف
+
+616
+00:44:00,400 --> 00:44:03,880
+dyes و salt، crystal violet and bile salt،
+
+617
+00:44:03,8
+
+667
+00:47:15,680 --> 00:47:19,740
+يبقى هذا بيميز الـ *E.coli* في المكونكي في الـ *AMP*
+
+668
+00:47:19,740 --> 00:47:23,570
+بتحول اللون للون أخضر. الـ *Non-Coliform*  أو الـ *Basilaria*
+
+669
+00:47:23,570 --> 00:47:26,030
+الـ *Non-Lactose Fermenter* ما بيصير *fermentation*
+
+670
+00:47:26,030 --> 00:47:29,950
+للـ *Lactose*. فبالتالي بتظلها *transparent* or
+
+671
+00:47:29,950 --> 00:47:34,610
+*colorless*. لـ *Gram-Positive* بكتيريا. اتفقنا؟ أي ميديا
+
+672
+00:47:34,610 --> 00:47:38,030
+بتعمل *inhibition* لأي نوع من أنواع البكتيريا ما
+
+673
+00:47:38,030 --> 00:47:41,510
+بيصير *kill*; ما بتموت في هذه الميديا، فقط بيصير لـ
+
+674
+00:47:41,510 --> 00:47:48,560
+*growth inhibition*. طيب، نيجي للصور. طبق مكونكي. لاحظنا
+
+675
+00:47:48,560 --> 00:47:51,280
+فيه أنه يبقى الـ *Gram-Negative Bacteria*. طب
+
+676
+00:47:51,280 --> 00:47:54,040
+بدي أميز هل هي *lactose fermenter* or *non-lactose*
+
+677
+00:47:54,040 --> 00:47:59,840
+*fermenter*. الـ *E.coli* قلنا إنه بتحول لون الوسط
+
+678
+00:47:59,840 --> 00:48:03,540
+وكمان الكولوني للون الزهري نتيجة إنتاج كمية كبيرة من
+
+679
+00:48:03,540 --> 00:48:08,000
+الأسيد. فبالتالي عمل على حفظ الـ *pH* وتحول لون الوسط
+
+680
+00:48:08,000 --> 00:48:14,450
+للون اللي هو أحمر. هذا بالنسبة لـ *E.coli*. تمام. الـ
+
+681
+00:48:14,450 --> 00:48:17,210
+*Enterobacter* نفس الـ *E.coli*. الـ *E.coli* و الـ
+
+682
+00:48:17,210 --> 00:48:19,090
+*Enterobacter* و الـ *Klebsiella* و الـ *Citrobacter*
+
+683
+00:48:19,090 --> 00:48:21,890
+نفس إيش؟ بتاعته لون *pink to red*. لكن اللي بيفرق
+
+684
+00:48:21,890 --> 00:48:25,310
+الـ *E.coli* إنها من لاحظ الـ *surrounding media* و
+
+685
+00:48:25,310 --> 00:48:30,350
+الكولوني لونها أحمر. تمام. بالنسبة للـ *Enterobacter*
+
+686
+00:48:30,350 --> 00:48:36,330
+برضه نمت، لاحظت أن اللون *pink to red*. يبقى من عائلة
+
+687
+00:48:36,330 --> 00:48:40,910
+الـ *coliform*، لاكتوز أو نحكي لاكتوز *fermented*.
+
+688
+00:48:40,910 --> 00:48:45,450
+برودوكشن السالمونيلا. تنتهى الـ *Non-lactose*
+
+689
+00:48:45,450 --> 00:48:47,870
+*fermentation*. يبقى مش هتعمل *fermentation* للاكتوز.
+
+690
+00:48:47,870 --> 00:48:51,990
+هتظهر اللون، الكولوني لونها *colorless* or *transparent*.
+
+691
+00:48:51,990 --> 00:48:55,330
+الاستافيلوكوكس أوريوس. مين؟ لـ *Gram-positive bacteria*.
+
+692
+00:48:55,330 --> 00:48:58,570
+فبالتالي مش هلاقي أي نمو على الطبق لأن هاي الـ
+
+693
+00:48:58,570 --> 00:49:01,970
+*media* بيصير فيها *inhibition* لـ *Gram-positive*
+
+694
+00:49:01,970 --> 00:49:07,210
+*bacteria*. طيب، لو سألنا، طبعاً هان هي معنى المادة نتيجة
+
+695
+00:49:07,210 --> 00:49:11,170
+أنه صار *inhibition* بواسطة الـ *crystal violet* و الـ
+
+696
+00:49:11,170 --> 00:49:18,670
+*bile salt*. لو سألنا سؤال: إن السيودوموناس، السيودوموناس
+
+697
+00:49:18,670 --> 00:49:22,470
+على طبق الـ *AMP* أو طبق المكونكي، طبعاً هي الـ *Gram*
+
+698
+00:49:22,470 --> 00:49:26,350
+*Negative Bacteria*. فأكيد السيودوموناس هتنمو على الـ
+
+699
+00:49:26,350 --> 00:49:30,130
+*AMP* والمكونكي. وإحنا قلنا الاثنين مش بس للـ
+
+700
+00:49:30,130 --> 00:49:32,610
+*Enterobacteriaceae*. هي بتنمي كل *Gram-Negative*
+
+701
+00:49:32,610 --> 00:49:36,090
+*Bacteria*. فعشان هيك لازم أعمل فحوصات، فحوصات الـ
+
+702
+00:49:36,090 --> 00:49:39,340
+*Oxidase*, فحوصات الـ *Glucose Fermentation* عشان أتأكد
+
+703
+00:49:39,340 --> 00:49:42,400
+إنها من عائلة الانتيروباكتريازية. وبعدين أحكي هل
+
+704
+00:49:42,400 --> 00:49:45,700
+هي من عائلة الكوليفورم أو من الـ *non-coliform*.
+
+705
+00:49:45,700 --> 00:49:49,600
+السوداموناس تعتبر *Gram-negative* بكتيريا. أكيد هتنمو
+
+706
+00:49:49,600 --> 00:49:55,140
+على طبق المكونكي أو الـ *AMP*. طيب، إيش هتعطي كولوني؟
+
+707
+00:49:55,140 --> 00:49:58,640
+السوداموناس بالأصل هي *non-glucose fermenter*.
+
+708
+00:49:58,640 --> 00:50:02,800
+فبالتالي هي *non-lactose*. بما إنها ما عملت *glucose*
+
+709
+00:50:02,800 --> 00:50:05,540
+*fermentation* لأبسط سكر، فبالتالي مش هتعمل
+
+710
+00:50:05,540 --> 00:50:10,240
+*fermentation* للـ *disaccharide* اللاكتوز. فبالتالي
+
+711
+00:50:10,240 --> 00:50:14,100
+تعتبر *non-lactose fermenter*. يبقى على طبق المكونكي
+
+712
+00:50:14,100 --> 00:50:18,280
+وعلى طبق الـ *A&B*، الـ *Pseudomonas* هتعامل معاملة الـ *non-*
+
+713
+00:50:18,280 --> 00:50:22,140
+-*lactose fermenter*. هيكون على المكونكي *colorless*.
+
+714
+00:50:22,140 --> 00:50:25,960
+على الـ *A&B* ممكن إنها تكون *colorless* وممكن تاخد لون
+
+715
+00:50:25,960 --> 00:50:31,400
+الميديا. هذا بالنسبة للـ *Pseudomonas*. فإحنا نكون خلصنا
+
+716
+00:50:32,240 --> 00:50:34,460
+معمل الـ "Selective" والـ "Differential Media".
+
+717
+00:50:34,460 --> 00:50:37,740
+تعرفنا على ٣ ميديا: مكونكي، *K*, *A*, *M*, *B*، مانيتول،
+
+718
+00:50:37,740 --> 00:50:40,220
+*asparagine agar*. كل ميديا من الـ "Selective" من الـ
+
+719
+00:50:40,220 --> 00:50:43,980
+"Differential Agent". أش بتعطي؟ كيف بتفرق بين مين
+
+720
+00:50:43,980 --> 00:50:47,480
+ومين؟ كل، مثلاً، الـ "Lactose Fermenter"، الـ "Non-
+
+721
+00:50:47,480 --> 00:50:51,880
+Lactose Fermenter"، شو اللون في كل حالة؟ كذلك في
+
+722
+00:50:51,880 --> 00:50:54,420
+المانيتول، اللي هي المانيتول "Fermenter" والـ
+
+723
+00:50:54,420 --> 00:50:57,560
+"Non-Mannitol Fermenter". في كل منهم أش بتعطي لون.
+
+724
+00:50:58,440 --> 00:51:03,920
+أتمنى الأمور والشرح يكون واضح. يعطيكم العافية.
+
+725
+00:51:03,920 --> 00:51:06,540
+والسلام عليكم ورحمة الله وبركاته.
diff --git a/PL9fwy3NUQKwZWgAb_V1ATlRRsH-6i3cPC/WtgYOo5DhhI_postprocess.srt b/PL9fwy3NUQKwZWgAb_V1ATlRRsH-6i3cPC/WtgYOo5DhhI_postprocess.srt
new file mode 100644
index 0000000000000000000000000000000000000000..788df139042be6aacafa373c8bcd642432f862bc
--- /dev/null
+++ b/PL9fwy3NUQKwZWgAb_V1ATlRRsH-6i3cPC/WtgYOo5DhhI_postprocess.srt
@@ -0,0 +1,2900 @@
+1
+00:00:02,060 --> 00:00:05,800
+السلام عليكم ورحمة الله وبركاته يعطيكم العافية
+
+2
+00:00:05,800 --> 00:00:09,280
+جميعًا اليوم المحاضرة بانوان Selective and
+
+3
+00:00:09,280 --> 00:00:13,560
+Differential Media في المحاضرة أو في المعامل
+
+4
+00:00:13,560 --> 00:00:17,540
+السابقة اتكلمنا عن الـ Media عرفنا الـ Media
+
+5
+00:00:17,540 --> 00:00:22,180
+وصنفنا الـ Media أو قسمنا الـ Media حسب الـ
+
+6
+00:00:22,180 --> 00:00:25,800
+Function حسب الـ Consistency حسب الـ Nutrient
+
+7
+00:00:25,800 --> 00:00:31,000
+وذكرنا عدة تصطيفات تحت كل صنف من هذه الأصناف
+
+8
+00:00:31,570 --> 00:00:36,270
+بالنسبة للـ function قلنا ممكن انها تكون general
+
+9
+00:00:36,270 --> 00:00:40,210
+media ممكن انها تكون transport media ممكن انها
+
+10
+00:00:40,210 --> 00:00:43,030
+تكون selective media ممكن انها تكون differential
+
+11
+00:00:43,030 --> 00:00:48,090
+media اليوم ماعملنا هنحكي فيه عن ال selective و ال
+
+12
+00:00:48,090 --> 00:00:52,610
+differential media هنشرح شو يعني selective شو يعني
+
+13
+00:00:52,610 --> 00:00:55,610
+differential و أمثلة على media selective و
+
+14
+00:00:55,610 --> 00:01:00,390
+differential media طيب بالأول خلينا نتكلم شو يعني
+
+15
+00:01:00,390 --> 00:01:04,740
+selectiveSelective بالعربي معناها انتقائي او
+
+16
+00:01:04,740 --> 00:01:10,780
+اختياري بدنا نختار و نمي نوع معين من الميكروبات
+
+17
+00:01:10,780 --> 00:01:14,800
+يبقى هي selection for the growth of certain
+
+18
+00:01:14,800 --> 00:01:19,160
+microorganism بدنا نمي نوع معين من الميكروبات
+
+19
+00:01:19,160 --> 00:01:25,360
+differential يعني تميزي بدي اميز كل نوع من هاي
+
+20
+00:01:25,360 --> 00:01:30,340
+الميكروبات اللي نمت بلون معينيبقى differential to
+
+21
+00:01:30,340 --> 00:01:34,720
+distinguish every group or species يبقى ال
+
+22
+00:01:34,720 --> 00:01:38,620
+selective راح ينمي ال group من ال micro organism
+
+23
+00:01:38,620 --> 00:01:43,660
+راح ييجي ال differential يعطي كل species في هذا ال
+
+24
+00:01:43,660 --> 00:01:49,000
+group إشي مميز عن التاني هذا بالنسبة لمعنى
+
+25
+00:01:49,000 --> 00:01:53,080
+selective و differential mediaممكن الميديا تكون
+
+26
+00:01:53,080 --> 00:01:57,920
+selective لحال ممكن تكون الميديا differential لحال
+
+27
+00:01:57,920 --> 00:02:02,100
+ممكن تكون الميديا selective و differential بنفس
+
+28
+00:02:02,100 --> 00:02:05,680
+الوقت يعني انها تنمي جروب من ال micro organism
+
+29
+00:02:05,680 --> 00:02:11,180
+بعدين الميديا نفسها راح اتميز تعمل differentiate
+
+30
+00:02:11,180 --> 00:02:14,700
+تعطي كل ال species في هذا الجروب اشي مميز عن
+
+31
+00:02:14,700 --> 00:02:19,080
+التاني طيب
+
+32
+00:02:19,440 --> 00:02:22,320
+in addition to general herbivores media which
+
+33
+00:02:22,320 --> 00:02:25,040
+allow the growth of most type of bacteria
+
+34
+00:02:25,040 --> 00:02:29,120
+microbiologists use specialized media to identify
+
+35
+00:02:29,120 --> 00:02:34,660
+or isolate specific group of bacteria شو الهدف من
+
+36
+00:02:34,660 --> 00:02:38,920
+ال media الهدف من ال media أني أنمي معظم
+
+37
+00:02:38,920 --> 00:02:42,320
+الميكروبات allow the growth of most type of
+
+38
+00:02:42,320 --> 00:02:47,660
+bacteria بدي أنا أنمي معظم الميكروبات لكن بقوللي
+
+39
+00:02:47,660 --> 00:02:54,770
+أنهالـ Microbiologist بيختار ال media أو بيختار
+
+40
+00:02:54,770 --> 00:02:59,750
+media خاصة عشان يعزل أنواع معينة من الميكروبات
+
+41
+00:02:59,750 --> 00:03:06,110
+ويعمل تعريف لإلها احنا قلنا او مرمعنا قبل كده ان
+
+42
+00:03:06,110 --> 00:03:10,490
+ال microbiologist بيختار ال media حسب نوع العينة
+
+43
+00:03:10,490 --> 00:03:14,630
+بكون انا عارفة ان والله مثلا عينة ال urine ال most
+
+44
+00:03:14,630 --> 00:03:19,920
+commonلأ ال pathogen in urine X وY وZ فبالتالي أنا
+
+45
+00:03:19,920 --> 00:03:25,440
+بختار media اتنمي هدول البكتيريا ال most common
+
+46
+00:03:25,440 --> 00:03:30,520
+pathogen in urine عشان اعزل هذه الأنواع المعينة من
+
+47
+00:03:30,520 --> 00:03:34,220
+الميكروبات وأعمل لها تعريف selective and
+
+48
+00:03:34,220 --> 00:03:38,620
+differential media are used to isolate or identify
+
+49
+00:03:38,620 --> 00:03:42,400
+particular organism أكيد ال selective و ال
+
+50
+00:03:42,400 --> 00:03:46,390
+differential mediaتستخدم لعزل نوع معين من
+
+51
+00:03:46,390 --> 00:03:51,430
+الميكروبات وتعريفها selective media هنمعرف شو هي
+
+52
+00:03:51,430 --> 00:03:55,030
+ال selective media وانا ذكرت التعريف في بداية
+
+53
+00:03:55,030 --> 00:04:00,070
+المعمل allow certain type of organism to grow بدها
+
+54
+00:04:00,070 --> 00:04:04,990
+تسمح لنوع معين فقط انه ينمو and inhibit the growth
+
+55
+00:04:04,990 --> 00:04:09,950
+of other organism وباقي ال organism هتعمله
+
+56
+00:04:09,950 --> 00:04:14,270
+inhibition مش هتعمله killهتعمله inhibition ل
+
+57
+00:04:14,270 --> 00:04:18,750
+growth of other organism طيب بقول ال selectivity
+
+58
+00:04:18,750 --> 00:04:24,090
+ال selective agent إيش هو؟ for example organism
+
+59
+00:04:24,090 --> 00:04:27,910
+that can utilize a given sugar are easily secreted
+
+60
+00:04:27,910 --> 00:04:31,050
+by making that sugar that only carbon source in
+
+61
+00:04:31,050 --> 00:04:35,750
+the mediaعلى سبيل المثال ذاكرها ال media مرت معنى
+
+62
+00:04:35,750 --> 00:04:39,770
+اللي هي ال Simon-Cytrate Agaric قولنا هذه ال media
+
+63
+00:04:39,770 --> 00:04:45,130
+تعتبر selective media ليش selective media لأن فقط
+
+64
+00:04:45,130 --> 00:04:48,790
+ال organism اللي عنده القدرة على استهلاك ال
+
+65
+00:04:48,790 --> 00:04:54,230
+citrate كمصدر لل carbon فقط هو اللي هينمو او فقط
+
+66
+00:04:54,230 --> 00:04:58,410
+هي البكتيريا اللي هتنمو لأن ال citrate هو المصدر
+
+67
+00:04:58,410 --> 00:05:03,950
+الوحيد كان لل carbon في هذه ال mediaعشان هيك يعتبر
+
+68
+00:05:03,950 --> 00:05:08,990
+ال sodium citrate في ميدية السمن سيطرات أجار هو ال
+
+69
+00:05:08,990 --> 00:05:14,550
+selective agent هو اللي خلّى انه فقط نوع معين
+
+70
+00:05:14,550 --> 00:05:17,290
+البكتيريا اللي عندها القدرة على استهلاك ال citrate
+
+71
+00:05:17,290 --> 00:05:21,130
+هي اللي تنمو أما ال other organism مش هيقدر ينمو
+
+72
+00:05:21,130 --> 00:05:24,870
+هيعمله inhibition أو مش هيقدر ينمو في الأصل كمان
+
+73
+00:05:24,870 --> 00:05:29,860
+تمام؟مثال على selective media أو وضّح فكرة شو يعني
+
+74
+00:05:29,860 --> 00:05:33,240
+selective media شو أنا بضيف في هذه ال media عشان
+
+75
+00:05:33,240 --> 00:05:40,300
+تصير selective media on
+
+76
+00:05:40,300 --> 00:05:43,560
+the other hand selective inhibition of some type
+
+77
+00:05:43,560 --> 00:05:47,900
+of microorganism can be achieved by adding dyes
+
+78
+00:05:47,900 --> 00:05:52,440
+antibiotic salt or specific inhibitor which affect
+
+79
+00:05:52,440 --> 00:05:55,740
+the metabolism or enzyme system of the organism
+
+80
+00:05:56,450 --> 00:06:06,550
+بقول لي ال selective agent ممكن يكون salt ممكن
+
+81
+00:06:06,550 --> 00:06:11,150
+يكون dyes ممكن يكون سبغات ال selective agent ممكن
+
+82
+00:06:11,150 --> 00:06:19,390
+يكون dyes ممكن يكون antibiotic ممكن يكون salt all
+
+83
+00:06:19,390 --> 00:06:22,530
+specific inhibitor بتأثر على ال metabolism و ال
+
+84
+00:06:22,530 --> 00:06:28,060
+enzyme ل الأورجانيزمهدول هم اللي هيخلوا او هيعملوا
+
+85
+00:06:28,060 --> 00:06:31,700
+inhibition ل ال other organism الستان و ال
+
+86
+00:06:31,700 --> 00:06:36,680
+antibiotic و السون فبالتالي ال other organism هي
+
+87
+00:06:36,680 --> 00:06:41,440
+اللي هتنمو فقط يبقى هذا يعتبر selective inhibition
+
+88
+00:06:41,440 --> 00:06:47,320
+بيعمل inhibition ل organism معين فبالتالي مش هيقدر
+
+89
+00:06:47,320 --> 00:06:52,260
+ينمو فقط ال other organism هو اللي هينمو ذا كل
+
+90
+00:06:52,260 --> 00:06:57,670
+أمثلةعلى سبيل المثال، مجموعة تحتوي على بوتاسيوم
+
+91
+00:06:57,670 --> 00:07:04,010
+توليرايت أو سوديوم أزايت أو ثاليوم أسيتات هدول
+
+92
+00:07:04,010 --> 00:07:09,950
+طبعا بتركيز واحد من عشرة لخمسة من عشرة سينهيبط
+
+93
+00:07:09,950 --> 00:07:15,490
+أكتر من جرام بكتيرية ناجحةتمام هدول هم ال
+
+94
+00:07:15,490 --> 00:07:19,290
+potassium tolerate و Sodium Azide و Phthalium
+
+95
+00:07:19,290 --> 00:07:24,270
+Acetate هدول كلهم يعتبروا ك selective agent
+
+96
+00:07:24,270 --> 00:07:30,010
+بيعملوا inhibition لجرام negative bacteria يبقى
+
+97
+00:07:30,010 --> 00:07:34,050
+هيكونوا لو أنا media اضفت عليها هدول التلاتة ال
+
+98
+00:07:34,050 --> 00:07:38,500
+salt طبعا هدول يعتبروا saltمن الأمثلة على الـ
+
+99
+00:07:38,500 --> 00:07:41,400
+Selective Inhibition بيعملوا Inhibition لـ Growth
+
+100
+00:07:41,400 --> 00:07:44,940
+of Gram Negative Bacteria يبقى هيكونوا Selection
+
+101
+00:07:44,940 --> 00:07:49,240
+لمين للجرام Positive Bacteria هيكونوا Selective
+
+102
+00:08:19,630 --> 00:08:26,070
+هدول طبعا ال salt أي
+
+103
+00:08:26,070 --> 00:08:30,630
+ميديا بتحتوي على هدول ال salt هاي الأملح بتعمل هاي
+
+104
+00:08:30,630 --> 00:08:33,970
+الأملح inhibition لجرام negative بكتيريا بالتالي
+
+105
+00:08:33,970 --> 00:08:37,770
+هتسمح بنمول جرام positive بكتيريا selection of
+
+106
+00:08:37,770 --> 00:08:43,230
+جرام positive بكتيرياطيب media supplement with
+
+107
+00:08:43,230 --> 00:08:46,970
+penicillin ال penicillin يعتبر antibiotic هاي مثال
+
+108
+00:08:46,970 --> 00:08:49,510
+احنا قولنا ممكن يكون dyes ممكن يكون antibiotic
+
+109
+00:08:49,510 --> 00:08:53,160
+ممكن يكون saltأمثلة على الـ Salt هيها التلاتة هذول
+
+110
+00:08:53,160 --> 00:08:56,960
+يعتبروا Salt قولنا بتعمل Inhibition لـ Growth of
+
+111
+00:08:56,960 --> 00:09:00,100
+Gram Negative Bacteria طيب الميديا اللي فيها
+
+112
+00:09:00,100 --> 00:09:03,520
+Penicillin ال Penicillin يعتبر Antibiotic هي مثال
+
+113
+00:09:03,520 --> 00:09:07,900
+على ال Antibiotic او ال crystal violet ال crystal
+
+114
+00:09:07,900 --> 00:09:13,240
+violet يعتبر Dyes استعينى يبقى هيك ذكرنا كل ذكرنا
+
+115
+00:09:13,240 --> 00:09:16,920
+مثال على كل نوع Dyes Antibiotic او Salt قولي ال
+
+116
+00:09:16,920 --> 00:09:20,580
+Penicillin و ال crystal violetبيعملوا inhibition
+
+117
+00:09:20,580 --> 00:09:25,380
+للجرام positive بكتيريا يبقى هي هتكون hand
+
+118
+00:09:25,380 --> 00:09:28,220
+selective لمين للجرام negative بكتيريا
+
+119
+00:09:47,230 --> 00:09:51,410
+يبقى أي ميديا فيها بنيسيلان وcrystal violet هتنمي
+
+120
+00:09:51,410 --> 00:09:56,050
+فقط لجرام نيجاتيف بكتيريا selective of جرام
+
+121
+00:09:56,050 --> 00:09:59,610
+نيجاتيف بكتيريا هتعمل inhibition للجرام positive
+
+122
+00:09:59,610 --> 00:10:06,510
+بكتيريا والبنيسيلان احنا قلنا في بداية المعاملإنه
+
+123
+00:10:06,510 --> 00:10:11,330
+الاستهداف لإيلولا الروابط الـ cross-link ما بين
+
+124
+00:10:11,330 --> 00:10:14,510
+الـ بيبتيدوش لايكان في الـ gram positive بكتيريا
+
+125
+00:10:14,510 --> 00:10:17,110
+فبالتالي احنا قلنا إنه هيأثر على الـ gram positive
+
+126
+00:10:17,110 --> 00:10:20,390
+بكتيريا ومش هيأثر على الـ gram negative بكتيريا
+
+127
+00:10:20,390 --> 00:10:26,740
+هذا كان ال action تبعه للبنسلان طيبهنذاكر إنه
+
+128
+00:10:26,740 --> 00:10:30,040
+ميديات نفس الكلام اللي فوق توليرايت أجار إيش يعني
+
+129
+00:10:30,040 --> 00:10:33,940
+توليرايت أجار يعني ميديا فيها بوتاسيوم توليرايت
+
+130
+00:10:33,940 --> 00:10:37,240
+قلنا هي ميديا بتعمل inhibition لليجرام negative
+
+131
+00:10:37,240 --> 00:10:41,480
+بكتيريا يبقى هي selective لليجرام positive بكتيريا
+
+132
+00:10:41,480 --> 00:10:45,320
+is used to select polygram positive organism هي
+
+133
+00:10:45,320 --> 00:10:50,260
+كاتب هي ميديا بتعمل هي بتختار بتعمل اختيار لليجرام
+
+134
+00:10:50,260 --> 00:10:53,500
+positive بكتيريا اتفقنا فوق هذا الكلام على هذا
+
+135
+00:10:53,500 --> 00:10:57,370
+الكلامبما انها بتعمل inhibition لجرام negative
+
+136
+00:10:57,370 --> 00:11:01,930
+بكتيريا يبقى هي select for gram positive بكتيريا
+
+137
+00:11:01,930 --> 00:11:05,630
+and nutrient agar supplement with penicillin لو
+
+138
+00:11:05,630 --> 00:11:10,290
+nutrient agar ضفت عليها penicillin احنا قلنا ال
+
+139
+00:11:10,290 --> 00:11:13,370
+penicillin بيعمل inhibition لجرام positive بكتيريا
+
+140
+00:11:13,370 --> 00:11:18,050
+يبقى هو select for gram negative organism هيك
+
+141
+00:11:18,050 --> 00:11:25,190
+بيكون المعلومات واضحةيعني الكلام هذا تقريبًا ذاكر
+
+142
+00:11:25,190 --> 00:11:27,930
+«هان» إنها بتعمل «inhibition» و «هان» ذاكر إنها
+
+143
+00:11:27,930 --> 00:11:32,430
+بتكون «selective» لمين يبقى أنا بحفظ ال «salt» هذا
+
+144
+00:11:32,430 --> 00:11:37,590
+بيعمل «inhibition» لمين يبقى بشكل بديهي بكون عارفة
+
+145
+00:11:37,590 --> 00:11:48,850
+«selective» لأي نوع من البكتيريا طيب
+
+146
+00:11:49,150 --> 00:11:52,290
+ذاكرنا شو يعني selective media نيجي لـ
+
+147
+00:11:52,290 --> 00:11:56,110
+differential media differential media doesn't
+
+148
+00:11:56,110 --> 00:12:00,250
+necessarily inhibit bacterial growth but instead
+
+149
+00:12:00,250 --> 00:12:03,430
+makes the bacteria look different بقول لي هي مش
+
+150
+00:12:03,430 --> 00:12:07,870
+بالضرورة انها تعمل inhibition لل growth للبكتيريا
+
+151
+00:12:07,870 --> 00:12:13,830
+لكن بتعمل تمييز لكل كولوني لكل species او كل group
+
+152
+00:12:13,830 --> 00:12:18,130
+بتخليها او بتعطيها شكل مميز كل group او كل species
+
+153
+00:12:18,600 --> 00:12:22,440
+أو every colony حتى differential media work best
+
+154
+00:12:22,440 --> 00:12:26,640
+with closely related organism and the differential
+
+155
+00:12:26,640 --> 00:12:32,100
+agent is what causes the bacteria look different
+
+156
+00:12:32,100 --> 00:12:35,200
+بقولي ال differential agent زي ما قولنا فيه
+
+157
+00:12:35,200 --> 00:12:37,880
+selective agent زي ال salt زي ال dye زي ال
+
+158
+00:12:37,880 --> 00:12:42,420
+antibiotic برضه في عندي differential agent مادة هي
+
+159
+00:12:42,420 --> 00:12:47,350
+اللي بتخلي البكتيريا مختلفةowing the presence of
+
+160
+00:12:47,350 --> 00:12:50,190
+certain dyes or chemical in the media the organism
+
+161
+00:12:50,190 --> 00:12:53,670
+will produce characteristic exchange or a growth
+
+162
+00:12:53,670 --> 00:12:56,610
+pattern that are used for identification or
+
+163
+00:12:56,610 --> 00:13:00,410
+differentiation بقول لي كمان ان هذا ال
+
+164
+00:13:00,410 --> 00:13:04,690
+differential agent ممكن يكون وجود هو عبارة عن وجود
+
+165
+00:13:04,690 --> 00:13:08,050
+presence of certain dyes or chemical موجودة في هذه
+
+166
+00:13:08,050 --> 00:13:11,550
+ال media ممكن يكون dyes ممكن يكون chemical موجود
+
+167
+00:13:11,550 --> 00:13:16,560
+في هذه ال media بيخلي ال organismيعمل نمو أو تغير
+
+168
+00:13:16,560 --> 00:13:21,420
+في النمو أو في نمط النمو وهذا هيساعد في ال
+
+169
+00:13:21,420 --> 00:13:25,840
+identification و ال differentiation هنفهم أكتر لما
+
+170
+00:13:25,840 --> 00:13:28,900
+نذكر أمثلة على selective و differential media
+
+171
+00:13:28,900 --> 00:13:31,800
+نتعرف مين هو ال selective agent كيف هذا ال
+
+172
+00:13:31,800 --> 00:13:35,380
+selective agent بيميز كل كولوني أو كل species أو
+
+173
+00:13:35,380 --> 00:13:39,860
+group في هذه ال mediaA variety of selective and
+
+174
+00:13:39,860 --> 00:13:42,840
+differential media are used in medical diagnostic
+
+175
+00:13:43,450 --> 00:13:47,110
+and water pollution laboratories and in food and
+
+176
+00:13:47,110 --> 00:13:51,530
+dairy laboratories بقوللي الـ Selective و الـ
+
+177
+00:13:51,530 --> 00:13:56,830
+Differential media بتستخدم في التشخيص الطبي زي ما
+
+178
+00:13:56,830 --> 00:14:02,130
+احنا كخصائي تحاليل طبية نستخدم الـ Differential و
+
+179
+00:14:02,130 --> 00:14:05,290
+الـ Selective media عشان اعمل identification
+
+180
+00:14:05,290 --> 00:14:11,510
+لmicro organism موجود لعندي عشان اعرف شو المسبب
+
+181
+00:14:11,510 --> 00:14:18,240
+للالتهابكمان يستخدم في تلوث المياه بيفلتره طبعا
+
+182
+00:14:18,240 --> 00:14:22,640
+المياه عشان يشوفوا الكوليفارم بعدين بيحطوها على
+
+183
+00:14:22,640 --> 00:14:29,220
+طبق ال AMB طبعا طبق ال AMB هنتعرف عليها في هذا ال
+
+184
+00:14:29,220 --> 00:14:34,480
+chapter ال AMB بتعطي ال equalize لون أخضر ال green
+
+185
+00:14:34,480 --> 00:14:38,680
+metallic chain فبالتالي هيك بتمييزها و بيسهل علي
+
+186
+00:14:38,680 --> 00:14:43,710
+تعريفها و ال identification لإلهاكمان برضه في اللي
+
+187
+00:14:43,710 --> 00:14:48,350
+هو مختبرات الـ Food ومراقبة وجودة الأغذية تقريبا
+
+188
+00:14:48,350 --> 00:14:51,310
+يعني في كل المختبرات تستخدم الـ Selective و الـ
+
+189
+00:14:51,310 --> 00:14:53,970
+Differential Media عشان تسهل عليها ال
+
+190
+00:14:53,970 --> 00:14:59,230
+identification والتعريف Some
+
+191
+00:14:59,230 --> 00:15:02,010
+media are both selective and differential احنا
+
+192
+00:15:02,010 --> 00:15:04,890
+قلنا في بعض أنواع ال media ممكن انها تكون
+
+193
+00:15:04,890 --> 00:15:08,750
+selective و differential في نفس الوقتthat is that
+
+194
+00:15:08,750 --> 00:15:12,090
+are able to select to select against the growth of
+
+195
+00:15:12,090 --> 00:15:17,570
+certain organism بقوللي هي بتسمح انها لنمو نوع
+
+196
+00:15:17,570 --> 00:15:21,970
+معين من الميكروبات او بتنمي نوع معين من الميكروبات
+
+197
+00:15:21,970 --> 00:15:27,240
+while the organism that do grow my eggssome
+
+198
+00:15:27,240 --> 00:15:31,580
+differential growth characteristic بقوللي ان اللي
+
+199
+00:15:31,580 --> 00:15:36,540
+اللي نمت من الميكروبات في هذه ال media بتمييزها
+
+200
+00:15:36,540 --> 00:15:43,080
+وبتعطيها اشي مختلف او لون مختلف عن ال عن كل group
+
+201
+00:15:43,080 --> 00:15:48,140
+او species there of the more common selective and
+
+202
+00:15:48,140 --> 00:15:51,360
+differential media are described below and will be
+
+203
+00:15:51,360 --> 00:15:55,840
+used in the laboratory exercise هنا في هذا الشابط
+
+204
+00:15:55,840 --> 00:15:59,780
+اذاكرتلت أمثلة على selective و differential media
+
+205
+00:15:59,780 --> 00:16:02,900
+أول
+
+206
+00:16:02,900 --> 00:16:07,680
+media هنتعرف عليها اللي هي ال money tall, salt,
+
+207
+00:16:07,880 --> 00:16:15,980
+agar بس هان في خطأ كتابي او هو ليس I money tall,
+
+208
+00:16:16,320 --> 00:16:22,820
+salt, agar هذه ال media هي selection of استفيل ككة
+
+209
+00:16:23,570 --> 00:16:28,190
+وبتعمل inhibition لـother organism يعني بتعمل
+
+210
+00:16:28,190 --> 00:16:33,710
+inhibition لجرام negative بكتيريا لجرام positive
+
+211
+00:16:33,710 --> 00:16:39,430
+غير لستافيلو كوكاية تبقى فقط بتنمي لستافيلو كوكاية
+
+212
+00:16:39,430 --> 00:16:44,650
+طيب شو اللي خلى في هذه ال media فقط لستافيلو
+
+213
+00:16:44,650 --> 00:16:48,530
+كوكاية انها اللي تنمو بقول في عندي selective agent
+
+214
+00:16:48,530 --> 00:16:51,990
+احنا قولنا selective agent ممكن يكون solved في هذه
+
+215
+00:16:51,990 --> 00:16:56,780
+ال mediaهو Sod التركيز العالي من ال Sodium
+
+216
+00:16:56,780 --> 00:17:02,500
+Chloride 7.5% NaCl هيعمل Inhibition لكل ال
+
+217
+00:17:02,500 --> 00:17:06,360
+organism مع دا الاستفيلوكوكاي لأن الاستفيلوكوكاي
+
+218
+00:17:06,360 --> 00:17:11,220
+من خصائصها انها بتتحمل درجة الملوحة العالية احنا
+
+219
+00:17:11,220 --> 00:17:16,800
+قلنا تركيز ال NaCl 85% من 100% تخيل انه يكون
+
+220
+00:17:16,800 --> 00:17:21,820
+التركيز 7.5% يبقى كل ال micro organism هيعملها
+
+221
+00:17:21,820 --> 00:17:25,370
+Inhibitionفقط لاستفيد الكوكايل عندها القدرة إنها
+
+222
+00:17:25,370 --> 00:17:29,450
+تتحمل درجة الملوحة العالية هي اللي هتنقل يبقى هذا
+
+223
+00:17:29,450 --> 00:17:31,990
+الـ Selective agent اللي هو التركيز العالي من
+
+224
+00:17:31,990 --> 00:17:36,950
+الأملح بالنسبة لـ Differential agent هذه ال media
+
+225
+00:17:36,950 --> 00:17:41,830
+تعتبر Selective and Differential agent مين هو الـ
+
+226
+00:17:41,830 --> 00:17:45,230
+Differential agent الـ Differential agent في هذه
+
+227
+00:17:45,230 --> 00:17:50,390
+ال media المانيتول المانيتول يعتبر sugar طيب
+
+228
+00:17:50,600 --> 00:17:53,660
+البكتيريا اللى نمت لستافيلو كوكاي في هذه الميديا
+
+229
+00:17:53,660 --> 00:17:57,860
+بقول لي لستافيلو كوكاي بدي اميزها بدي اميز ال
+
+230
+00:17:57,860 --> 00:18:01,600
+species للاستافيلو كوكاى Differentiate استافيلو
+
+231
+00:18:01,600 --> 00:18:05,260
+كوكاى ال species يبقى ال differential agent هو
+
+232
+00:18:05,260 --> 00:18:10,700
+المانيدال الشجر عشان نعرف كيف تعمل differentiate
+
+233
+00:18:10,700 --> 00:18:15,340
+بين الاستافيلو كوكاى ال species بنا نعرف ان في هذه
+
+234
+00:18:15,340 --> 00:18:20,770
+الميديا BH Indicator اللى هو الفينال Redالفيه non
+
+235
+00:18:20,770 --> 00:18:24,250
+-red مر معانا للمرة الرابعة اول مرة في اليوريا
+
+236
+00:18:24,250 --> 00:18:27,270
+المرة التانية فيه او اول مرة في ال fermentation
+
+237
+00:18:27,270 --> 00:18:30,630
+تانية مرة في اليوريا تالت مرة في ال triple sugar
+
+238
+00:18:30,630 --> 00:18:34,830
+iron agar وهي في ال manitol salt agar حدوده في ال
+
+239
+00:18:34,830 --> 00:18:38,130
+acid yellow في ال base بيكون non-red بقولي
+
+240
+00:18:38,130 --> 00:18:42,510
+البكتيريا اللى عندها الاستفيله كوكاي طبعا هي فقط
+
+241
+00:18:42,510 --> 00:18:45,670
+اللى هتتحمل الملوحة العالية فقط اللى هتنمو على ال
+
+242
+00:18:45,670 --> 00:18:50,110
+manitol salt agarبننميز الستافيلو كوكايا ال
+
+243
+00:18:50,110 --> 00:18:53,230
+species بقول ليه فى من الستافيلو كوكايا عندها
+
+244
+00:18:53,230 --> 00:18:57,210
+القدرة انها تعمل fermentation للمانيتال وفى non
+
+245
+00:18:57,210 --> 00:19:00,550
+manitol fermenter طيب البكتيريا اللى عندها القدرة
+
+246
+00:19:00,550 --> 00:19:05,350
+انها تعمل fermentation للمانيتال هتعمل ferment
+
+247
+00:19:05,350 --> 00:19:08,270
+للمانيتال طبعا المانيتال شجر هيصله fermentation
+
+248
+00:19:09,320 --> 00:19:12,860
+هتعمله fermentation هتعمل fermentation للمانيتال
+
+249
+00:19:12,860 --> 00:19:17,500
+نواتج عملية ال fermentation acid هتعمل على خفض ال
+
+250
+00:19:17,500 --> 00:19:23,680
+pH هيتحول لون الوسط للون yellow يبقى colonies
+
+251
+00:19:23,680 --> 00:19:28,740
+surrounded by yellow zone هي بتكون معناها انه
+
+252
+00:19:28,740 --> 00:19:32,160
+manitol fermenter من الأمثلة على ال manitol
+
+253
+00:19:32,160 --> 00:19:36,510
+fermenter اللي استفيله gas aureusطب لو البكتيريا
+
+254
+00:19:36,510 --> 00:19:39,730
+الاستفيله ككاى ال species ماعندها القدرة انها تعمل
+
+255
+00:19:39,730 --> 00:19:45,610
+fermentation للمانيتال مش هيتم انتج اي تغير في
+
+256
+00:19:45,610 --> 00:19:50,690
+اللون فبالتالي هيظلوا ال colony لونها colorless و
+
+257
+00:19:50,690 --> 00:19:54,510
+لون الطبق لونه pink من الأمثلة عليها الاستفيله
+
+258
+00:19:54,510 --> 00:19:58,950
+ككاس ابدامتر يبقى لو مسكنا طبق manitol salt agar
+
+259
+00:19:58,950 --> 00:20:03,510
+لقنا بكتيريا نامية يبقى بعرف انه هيستفيله ككاىلأن
+
+260
+00:20:03,510 --> 00:20:08,270
+هذه الميديا مختارة من استفيله و ككاية فقط اللي
+
+261
+00:20:08,270 --> 00:20:13,010
+هتنمو لستفيله و ككاية طيب أعطتني لون أصفر يبقى
+
+262
+00:20:13,010 --> 00:20:17,750
+بعرف أنه عملت Manitol Fermenter شو يعني Manitol
+
+263
+00:20:17,750 --> 00:20:20,690
+Fermenter يعني عملت fermentation للمانيتل اتكون
+
+264
+00:20:20,690 --> 00:20:25,510
+أسد عمل على حفظ ال BH فبالتالي اتحول لون الوسط
+
+265
+00:20:25,510 --> 00:20:29,730
+للون أصفريبقى لون الوسط لون الـmedia لونها زهري
+
+266
+00:20:29,730 --> 00:20:34,210
+هلاقي ان الـmedia تحول لونها لللون الأصفر وممكن
+
+267
+00:20:34,210 --> 00:20:37,170
+إذا اتكون كمية كبيرة من الأسد ألاقي الـcolony
+
+268
+00:20:37,170 --> 00:20:40,650
+نفسها تحولت للون الأصفر من الأمثلة عليها
+
+269
+00:20:40,650 --> 00:20:44,830
+الاستافيلوكوكا او الاسم طيب لو مسكت الطبق ولاقيت
+
+270
+00:20:44,830 --> 00:20:48,390
+والله عندي كولوني لونها colorless لكن الطبق
+
+271
+00:20:48,390 --> 00:20:51,590
+حوالينه ضاله لونه pink يبقى بعرف ان البكتيريا
+
+272
+00:20:51,590 --> 00:20:54,630
+استافيلوكوكاي لأن الـmedia selection of
+
+273
+00:20:54,630 --> 00:20:59,080
+استافيلوكوكاطيب لونها colorless و لون ال media
+
+274
+00:20:59,080 --> 00:21:02,900
+دلوا لونه pink يبقى بعرف انه هي non manitol
+
+275
+00:21:02,900 --> 00:21:07,400
+fermenter ماعملت fermentation للمنيتول ماتغير لون
+
+276
+00:21:07,400 --> 00:21:12,500
+ال media دلوا لون ال media pink و دل لون ال كله
+
+277
+00:21:12,500 --> 00:21:16,580
+colorless من الأمثلة عليها لسه فيه كوكس epidermis
+
+278
+00:21:16,580 --> 00:21:22,920
+هذا بالنسبة لمنيتول salt agaricManitol salt agar
+
+279
+00:21:22,920 --> 00:21:25,580
+is a selective media هي selective و differential
+
+280
+00:21:25,580 --> 00:21:29,960
+media لكن هو هان بده يشرحش يعني selective media
+
+281
+00:21:29,960 --> 00:21:33,540
+used for isolation of pathogenic Staphylococci
+
+282
+00:21:33,540 --> 00:21:37,680
+تستخدم لعزل الـ Staphylococci لأن هي فقط بتنمي الـ
+
+283
+00:21:37,680 --> 00:21:41,080
+Staphylococci الميديا بتحتوي على Manitol ك
+
+284
+00:21:41,080 --> 00:21:44,380
+differential agent في non-red indicator كبيئة
+
+285
+00:21:44,380 --> 00:21:49,560
+Indicator 7.5% Sodium chloride ك selective agent
+
+286
+00:21:49,970 --> 00:21:53,470
+Ferment Manitol هو اللي هيعمل لديه Differentiate
+
+287
+00:21:53,470 --> 00:21:55,830
+هو الـDifferentiation اللي فيه Nordred هو الـBH
+
+288
+00:21:55,830 --> 00:21:58,770
+Indicator قولنا الـSelective الـHigh Salt
+
+289
+00:21:58,770 --> 00:22:01,970
+Concentration التركيز العالي من الأملح هيعمل
+
+290
+00:22:01,970 --> 00:22:05,510
+Inhibition لمعظم of most بكتيريا other than
+
+291
+00:22:05,510 --> 00:22:09,550
+Staphylococci معادل Staphylococci بقولي التركيز
+
+292
+00:22:09,550 --> 00:22:12,450
+العالي من الأملح مش هيعمل كله لـGram Negative
+
+293
+00:22:12,450 --> 00:22:16,470
+بكتيريا هيعمل Inhibition اتفقنا انه ما بيصير كله
+
+294
+00:22:16,470 --> 00:22:22,900
+بيصير Inhibition لهلـ Growth لـ Other Organism On
+
+295
+00:22:22,900 --> 00:22:27,280
+Manitouin Salt Agar باثوجينك إستفيلوكوكس أوريس
+
+296
+00:22:27,280 --> 00:22:30,740
+طبعا الباثوجينك من الإستفيلوكوكس هي الإستفيلوكوكس
+
+297
+00:22:30,740 --> 00:22:34,480
+أوريس produce small colonies surrounded by yellow
+
+298
+00:22:34,480 --> 00:22:39,400
+zone اتفقنا بتفرج طبعا هي بتكون small colony محيطة
+
+299
+00:22:39,400 --> 00:22:43,680
+باللون الأصفر نتيجة fermentation لـ Manitouinالسبب
+
+300
+00:22:43,680 --> 00:22:46,800
+في تغيير اللون دا تستفيل الكوكس Aureus هي ferment
+
+301
+00:22:46,800 --> 00:22:50,540
+the manitol producing an acid which is turned
+
+302
+00:22:50,540 --> 00:22:53,260
+trans the indicator from red to yellow هذا هو
+
+303
+00:22:53,260 --> 00:22:58,160
+السبب اللي بيخلي تستفيل الكوكس Aureus ان الكلوني
+
+304
+00:22:58,160 --> 00:23:01,320
+حولها لون أصفر نتيجة ال fermentation للمانيتل
+
+305
+00:23:01,320 --> 00:23:03,880
+تتكون acid عمل على خفض البيئة مش اتحول ال
+
+306
+00:23:03,880 --> 00:23:08,060
+indicator من red ليرد لكن الأخر لستفيل الكوكس ما
+
+307
+00:23:08,060 --> 00:23:12,650
+بتعمل fermentation للمانيتلمش هيتم إنتاج أي تغير
+
+308
+00:23:12,650 --> 00:23:17,490
+في لون الميديا اللي هو أصلا بنكلر تمام بيقول ال
+
+309
+00:23:17,490 --> 00:23:20,230
+growth of other types of bacteria is generally
+
+310
+00:23:20,230 --> 00:23:24,450
+inhibited ال organism الآخر غير الاستافيولوكوكاي
+
+311
+00:23:24,450 --> 00:23:29,430
+بيصير لها inhibition وليس killed طيب نيجي لكل نوع
+
+312
+00:23:29,430 --> 00:23:32,090
+من أنواع البكتيريا الاستافيولوكوكاس اورياس على
+
+313
+00:23:32,090 --> 00:23:37,080
+المانيتواس هولد أجار أكيدهى Salt Tolerant هتتحمل
+
+314
+00:23:37,080 --> 00:23:39,720
+الملوحة العالية فبالتالي هتنمو اى بكتيرى
+
+315
+00:23:39,720 --> 00:23:45,620
+Staphylococci طبعا هتنمو لانها هتتحمل درجة الملوحة
+
+316
+00:23:45,620 --> 00:23:51,420
+العالية تمام بالنسبة لStaphylococcus aureus ايش
+
+317
+00:23:51,420 --> 00:23:54,840
+هتعمل هتعمل fermentation للمنيطول فهيتحول لون ال
+
+318
+00:23:54,840 --> 00:23:58,160
+media للون يلو Staphylococcus epidermidis برضه
+
+319
+00:23:58,160 --> 00:24:01,400
+هتتحمل الملوحة العالية بما انها Staphylococciلكن
+
+320
+00:24:01,400 --> 00:24:04,620
+مش هتعمل fermentation للمانيتال فهيضلوا لون ال
+
+321
+00:24:04,620 --> 00:24:08,900
+media لون big ال micrococcus luteus not salt
+
+322
+00:24:08,900 --> 00:24:12,680
+tolerant doesn't growth of own manitol نمسح هذه
+
+323
+00:24:12,680 --> 00:24:17,680
+الجملة ال
+
+324
+00:24:17,680 --> 00:24:21,600
+micrococcus بقول لي نفس الاستفيله coccus ابتنموا
+
+325
+00:24:21,600 --> 00:24:25,540
+على ال manitol salt agar و بتعمل fermentation
+
+326
+00:24:25,540 --> 00:24:29,760
+للمانيتال طيب احنا قلنا انه فقط ال manitol salt
+
+327
+00:24:29,760 --> 00:24:34,230
+agarهي اللي بتنمو على ميدية الـ Manitoulin salt
+
+328
+00:24:34,230 --> 00:24:38,490
+agar كيف بنحكي أنه ال micro ككس نفس لسه فيه ككس
+
+329
+00:24:38,490 --> 00:24:43,670
+aureus بتنمو و بتعطي اللون الأصفر بدنا نعرف معلومة
+
+330
+00:24:43,670 --> 00:24:47,530
+never ever trust is in selectivity of the
+
+331
+00:24:47,530 --> 00:24:51,070
+selective media مابدي أثق في ال selective media
+
+332
+00:24:51,070 --> 00:24:54,370
+لازم انا اعمل فحوصات اعمل جرامستين اعمل فحوصات
+
+333
+00:24:54,370 --> 00:24:58,650
+كيميائية هي بتسهل علي التعريف لكن انامش من حد ما
+
+334
+00:24:58,650 --> 00:25:01,630
+افتح طبق الـ Manitoula Salt Agar أشوف اللون أصفر
+
+335
+00:25:01,630 --> 00:25:05,350
+خلص على طول الـ Staphylococcus Aureus لأ لازم أعمل
+
+336
+00:25:05,350 --> 00:25:09,990
+إجرامستان لازم أمشي فحوصات لازم أمشي كل الفحوصات
+
+337
+00:25:09,990 --> 00:25:13,690
+حتى أوصل لتعريفي نهائي فقط بعرف أنه هي عملت
+
+338
+00:25:13,690 --> 00:25:17,410
+fermentation لالـ Manitoula من ميدية الاستفيلو من
+
+339
+00:25:17,410 --> 00:25:21,250
+ميدية الـ Manitoula Salt Agar يبقى هنا فيه طبعا
+
+340
+00:25:21,250 --> 00:25:24,850
+دراسات حديثة بتأكد أنه ال micrococcus نفس لـ
+
+341
+00:25:24,850 --> 00:25:28,320
+Staphylococcus Aureus علىالـ Manifold Salt Agar
+
+342
+00:25:28,320 --> 00:25:33,080
+بتنمو وبتعطي اللون الأصفر فبالتالي بدنا نستفيد من
+
+343
+00:25:33,080 --> 00:25:36,100
+هذه المعلومة إنه «never ever trust in selectivity
+
+344
+00:25:36,100 --> 00:25:41,440
+of selective media»
+
+345
+00:25:41,440 --> 00:25:46,960
+طيب بالنسبة للصور هذه الصور ممكن إنه يجينا في
+
+346
+00:25:46,960 --> 00:25:49,820
+الامتحان هذا طبق Manifold Salt Agar ال media لون
+
+347
+00:25:49,820 --> 00:25:52,760
+ال media هيه لون زهري نفس اللون الموجود هنا اللي
+
+348
+00:25:52,760 --> 00:25:58,370
+أنا بأشهر عليهزراعة بكتيريتان بكتيريا أعطتني حولت
+
+349
+00:25:58,370 --> 00:26:02,610
+اللون حولان الكلوني لون أصفر وحتى لون الكلوني لون
+
+350
+00:26:02,610 --> 00:26:07,910
+أصفر لكن هنا بلاحظ أنه أظل اللون pink يبقى high
+
+351
+00:26:07,910 --> 00:26:12,270
+بما أنها نمط بحكي أنه هي استفيله ككاية طيب أو نحكي
+
+352
+00:26:12,270 --> 00:26:16,950
+سؤال tolerant اتحملت الملوحة العالية طيب نيجي ل
+
+353
+00:26:20,090 --> 00:26:23,110
+الـ fermentation بما انه اتحول اللون للون أصفر
+
+354
+00:26:23,110 --> 00:26:26,390
+يبقى بحكي Manitol Fermenter لكن هنا بحكي not
+
+355
+00:26:26,390 --> 00:26:31,010
+Manitol .. non Manitol Fermenter يبقى هذه احتمال
+
+356
+00:26:31,010 --> 00:26:34,950
+انها تكون مثال عليها لسه في الكوكس Aureus اللي
+
+357
+00:26:34,950 --> 00:26:38,770
+بتعطي هذا اللون وهي لسه في الكوكس Epidermis هنا
+
+358
+00:26:38,770 --> 00:26:41,470
+نفس الشيء هم اللاحظ اللون أصفر وهنا اللون أحمر
+
+359
+00:26:41,470 --> 00:26:44,910
+يبقى هاي Manitol Fermenter هاي Non Manitol
+
+360
+00:26:44,910 --> 00:26:50,160
+Fermenterهنا نلاحظ أن الطبق و الكلونيه صار لونها
+
+361
+00:26:50,160 --> 00:26:55,160
+أصفر يبقى هي بما أنها نمط يبقى هي salt tolerant من
+
+362
+00:26:55,160 --> 00:26:58,000
+الأمثلة عليها الاستفيل و الككاية طيب أعطت اللون
+
+363
+00:26:58,000 --> 00:27:01,800
+الأصفر يبقى manitol fermenter من الأمثلة عليها
+
+364
+00:27:01,800 --> 00:27:07,290
+الاستفيل و الككاسنمت يبقى البكتيريا salt tolerant
+
+365
+00:27:07,290 --> 00:27:12,790
+ما عضت اللون الأصفر، دا اللون ال media لونه pink و
+
+366
+00:27:12,790 --> 00:27:16,350
+ال colony كانت colorless، يبقى هي non-many-tol
+
+367
+00:27:16,350 --> 00:27:18,890
+fermenter من الأمثلة عليها الاستفيل و الككس
+
+368
+00:27:18,890 --> 00:27:22,890
+Epidermitis هاي ال media ماني تول sold أجار، لكن
+
+369
+00:27:22,890 --> 00:27:27,230
+ما لاحظت أي نمو، يبقى هي بالاصل ما اتحملت الملوحة
+
+370
+00:27:27,230 --> 00:27:30,590
+العالية، ماكان في عندي نمو، يبقى هاي احتمال أنها
+
+371
+00:27:30,590 --> 00:27:34,980
+تكون أي بكتيريا ما عدا الاستفيل و الككسبشكل عام
+
+372
+00:27:34,980 --> 00:27:37,640
+بنمحي الـ «micrococcus» قولنا لأنها بتنمو على
+
+373
+00:27:37,640 --> 00:27:41,960
+المانيتال سولت أجار ممكن نكتب اللي هي أي ميديا
+
+374
+00:27:41,960 --> 00:27:47,560
+إجرام positive كوكاي مثلاً الـ Clostridium البصلص
+
+375
+00:27:47,560 --> 00:27:52,380
+الستريبتو كوكاي أي بكتيريا إجرام positive أو إجرام
+
+376
+00:27:52,380 --> 00:27:56,380
+إجرام positive طبعاً من غير الاستافيرو كوكاي أو
+
+377
+00:27:56,380 --> 00:27:59,620
+إجرام negative بكتيريا هاي لو أنا نميتها على
+
+378
+00:27:59,620 --> 00:28:03,560
+المانيتال سولت أجار مش هتنمولأنها مش هتتحمل درجة
+
+379
+00:28:03,560 --> 00:28:07,380
+الملوحة العالية يبقى لو جيبنا في الامتحان سؤال انه
+
+380
+00:28:07,380 --> 00:28:12,000
+طبق منيطول salt agar لكن ما لاحظنا فيه نمو بنحكي
+
+381
+00:28:12,000 --> 00:28:17,660
+no growth because البكتيريا ماقدرة تتحمل non-salt
+
+382
+00:28:17,660 --> 00:28:21,020
+tolerant ماقدرة تتحمل الملوحة العالية يبقى هي أي
+
+383
+00:28:21,020 --> 00:28:24,660
+بكتيريا غير الاستافيلوكوكايو غير الميكروكوكاس
+
+384
+00:28:24,660 --> 00:28:28,580
+اذكري اي مثال مثلا gram negative bacteria
+
+385
+00:28:28,580 --> 00:28:34,080
+Sodamonas E.coli KlebsiellaStriptococci اي مثال من
+
+386
+00:28:34,080 --> 00:28:38,460
+ما عدا الاستفيلوكوكاي والمايكروكوكا هكذا نكون
+
+387
+00:28:38,460 --> 00:28:45,020
+خلصنا الأول ميديا اللي هي ال Manitol salt agar
+
+388
+00:28:45,020 --> 00:28:47,960
+selective و differential media تعرفنا شو ال
+
+389
+00:28:47,960 --> 00:28:51,700
+selective agent وشو ال differential agent طيب نيجي
+
+390
+00:28:51,700 --> 00:28:56,800
+لتاني ميديا using methylene blue agar هذه الميديا
+
+391
+00:28:56,800 --> 00:29:01,560
+برضه تعتبر selective و differential media تمامطيب
+
+392
+00:29:01,560 --> 00:29:04,140
+شو الـ selective agent في هذه الميديا الـ
+
+393
+00:29:04,140 --> 00:29:08,000
+selective agent هو اليوزين و الميثيليني بلو هدول
+
+394
+00:29:08,000 --> 00:29:11,560
+يعتبروا dyes قلنا ال selective agent ممكن يكون
+
+395
+00:29:11,560 --> 00:29:15,400
+dyes أمثلة على ميديا ال selective agent فيها dyes
+
+396
+00:29:15,400 --> 00:29:20,340
+هي اليوزين و الميثيليني بلو طيب هذه الميديا بقولي
+
+397
+00:29:20,340 --> 00:29:28,840
+بتعمل inhibition لجرام positive bacteria فبالتالي
+
+398
+00:29:28,840 --> 00:29:33,880
+هتكون selectionOr of Gram Negative Bacteria هتعمل
+
+399
+00:29:33,880 --> 00:29:38,220
+Inhibition لـ Gram Positive Bacteria فبالتالي
+
+400
+00:29:38,220 --> 00:29:41,680
+هتعمل Selection فقط اللي هتنمو هي لـ Gram Negative
+
+401
+00:29:41,680 --> 00:29:45,240
+Bacteria مين اللي عمل Inhibition لـ Gram Positive
+
+402
+00:29:45,240 --> 00:29:47,900
+Bacteria بقولي الـ Dice الايوزين والميثاليني ال
+
+403
+00:29:47,900 --> 00:29:50,840
+blue عملت Inhibition لـ Gram Positive Bacteria
+
+404
+00:29:50,840 --> 00:29:54,820
+ففقط اللي هتنمو هي لـ Gram Negative Bacteria هيك
+
+405
+00:29:54,820 --> 00:29:57,960
+أعرفنا مين اللي هينمو في هذه ال mediaالـ
+
+406
+00:29:57,960 --> 00:30:00,520
+Differential agent لـ Lactose كانت في الـ
+
+407
+00:30:00,520 --> 00:30:02,860
+Manitoulin Salt أجار الـ Manitoulin لكن هنا الـ
+
+408
+00:30:02,860 --> 00:30:06,800
+Lactose يبقى أي بكتيريا من الـ Gram Negative
+
+409
+00:30:06,800 --> 00:30:12,420
+بكتيريا نمت في هذا الطبق هيميز لي بين الـ Gram
+
+410
+00:30:12,420 --> 00:30:16,240
+Negative بكتيريا هل هي Lactose Fermenter أو Non
+
+411
+00:30:16,240 --> 00:30:20,220
+-Lactose Fermenter بشكل خاص هذه الميديا تستخدم للـ
+
+412
+00:30:20,220 --> 00:30:23,320
+Differentiate between coliform اللي هي تعتبر
+
+413
+00:30:23,320 --> 00:30:27,620
+Lactose Fermenternon-coliform bacteria فهي من عقلة
+
+414
+00:30:27,620 --> 00:30:30,640
+الـ Enterobacteriasi قلنا بتتقسم لـ coliform و non
+
+415
+00:30:30,640 --> 00:30:33,540
+-coliform أو lactose fermenter و non-lactose
+
+416
+00:30:33,540 --> 00:30:37,540
+fermenter ففي هذه الـ media أنا بفرق بين الـ
+
+417
+00:30:37,540 --> 00:30:39,920
+lactose fermenter من عقلة الـ Enterobacteriasi و
+
+418
+00:30:39,920 --> 00:30:42,740
+non-lactose fermenter من عقلة الـ Enterobacteriasi
+
+419
+00:30:42,740 --> 00:30:47,060
+لكن هذا لا يعني أن هذه البكتيريا فقط بتنمي الـ
+
+420
+00:30:47,060 --> 00:30:50,240
+Enterobacteriasi بتنمي كل gram negative بكتيريا
+
+421
+00:30:50,240 --> 00:30:56,610
+عشان هك لازم أعمل قبل ماأعمل مثلًا تعريف أو أحكي
+
+422
+00:30:56,610 --> 00:30:59,930
+إنه انتيرو بكترياسي لازم قبلها أعمل إيجرامستان
+
+423
+00:30:59,930 --> 00:31:03,050
+لازم قبلها أعمل فحص الـ Oxidase فحص الـGlucose
+
+424
+00:31:03,050 --> 00:31:06,370
+fermentation لأن في عندي صدوموناس في عندي Vibrio
+
+425
+00:31:06,370 --> 00:31:08,870
+cholera لازم أتأكد من هذه الأمور أعرف إن هي من
+
+426
+00:31:08,870 --> 00:31:12,110
+عائلة الانتيرو بكترياسي إذا أتأكدت إن هي من عائلة
+
+427
+00:31:12,110 --> 00:31:15,410
+الانتيرو بكترياسي بعرف بعدها هل هي lactose
+
+428
+00:31:15,410 --> 00:31:18,190
+fermenter أو non-lactosefermenter من خلال هذه
+
+429
+00:31:18,190 --> 00:31:22,130
+الـmediumالـ pH Indicator في هذه الميديا بقول لي
+
+430
+00:31:22,130 --> 00:31:26,710
+إن الصبغات بتشتغل as pH Indicator الـ Eucalyptus
+
+431
+00:31:26,710 --> 00:31:32,050
+Blue بتشتغل as pH Indicator طيب قلنا إن الـ Gram
+
+432
+00:31:32,050 --> 00:31:35,130
+Negative Bacteria هي اللي فقط بتنمو على الـ AMP
+
+433
+00:31:35,130 --> 00:31:38,230
+نمت بكتيريا يبقى و أعرف إن هي Gram Negative
+
+434
+00:31:38,230 --> 00:31:42,710
+Bacteria طيب بدي أميز هل هي lactose fermenter أو
+
+435
+00:31:42,710 --> 00:31:44,870
+non-lactose fermenter لأنه احنا قلنا الـ
+
+436
+00:31:44,870 --> 00:31:49,830
+differential agent هو اللاكتوز بقول لي لوالبكتيريا
+
+437
+00:31:49,830 --> 00:31:56,090
+طبعا كانت lactose fermenter هتنتج acid تمام هذا ال
+
+438
+00:31:56,090 --> 00:32:01,430
+acid اللي هو ال acid product هيربط بيه اليوزين and
+
+439
+00:32:01,430 --> 00:32:04,430
+methylene blue لأنه احنا قولنا هو as pH indicator
+
+440
+00:32:04,430 --> 00:32:10,490
+هيكون رابط ما بينهم تمام اسمها ال amide اللي هي
+
+441
+00:32:10,490 --> 00:32:16,040
+group هيعطي نلون metallic green sheetبتكون هذه
+
+442
+00:32:16,040 --> 00:32:19,400
+البكتيريا إذا على طبق ال amp اللي حاصة metallic
+
+443
+00:32:19,400 --> 00:32:24,560
+green chain بكون احتمال انها تكون ال E.coli لان ال
+
+444
+00:32:24,560 --> 00:32:28,340
+E.coli من الكوليفارم بتعمل fermentation للكتاز
+
+445
+00:32:28,340 --> 00:32:31,340
+بتكون أسد بقولي هذا الأسد بيرتبط بالإيوزين
+
+446
+00:32:31,340 --> 00:32:34,560
+والميثاليني بلو لكن الرابط ما بين الإيوزين
+
+447
+00:32:34,560 --> 00:32:39,400
+والميثاليني بلو بتكون رابط ما بينهم فبالتالي
+
+448
+00:32:39,400 --> 00:32:45,260
+بيعطيني اللون الأخضر ال metallic greenالـ Coliform
+
+449
+00:32:45,260 --> 00:32:47,960
+بكتيريا احنا قلنا E.coli, Klebsiella, Citrobacter,
+
+450
+00:32:48,080 --> 00:32:51,760
+Enterobacter الـ E.coli عرفنا انه اشي خاص في هاي
+
+451
+00:32:51,760 --> 00:32:54,100
+من عائلة الـ Lactose Fermenter بتعطي Metallic
+
+452
+00:32:54,100 --> 00:32:57,120
+Green Chain وعرفنا شو السبب طيب الـ Klebsiella
+
+453
+00:32:57,120 --> 00:32:59,160
+Citrobacter ال Enterobacter بقول بتعمل
+
+454
+00:32:59,160 --> 00:33:02,660
+fermentation للـ Lactose بتكون أسد زي الـ E.coli
+
+455
+00:33:02,660 --> 00:33:07,620
+بتربط بي اليوزين والميثيليني بلو بتعطي لون Pink to
+
+456
+00:33:07,620 --> 00:33:13,260
+Purple Colony أو Dark Purple Colonyلكن الـ E.coli
+
+457
+00:33:13,260 --> 00:33:16,820
+بتكون رابطة ما بين الإيزين والميسيليان بلو لكن هن
+
+458
+00:33:16,820 --> 00:33:20,720
+ما بتتكون رابطة فبنتج اللون البربل هن بتتكون رابطة
+
+459
+00:33:20,720 --> 00:33:23,540
+ما بين الإيزين والميسيليان بلو فبنتج اللون الـ
+
+460
+00:33:23,540 --> 00:33:26,940
+Metallic Green Sheen هذا بالنسبة للـ Lactose
+
+461
+00:33:26,940 --> 00:33:29,860
+Fermenter الـ Non-Lactose Fermenter بقول لي ما
+
+462
+00:33:29,860 --> 00:33:32,560
+بيصير اي fermentation للـ Lactose فبالتالي مش
+
+463
+00:33:32,560 --> 00:33:36,300
+هيتغير اللون ال media ومش هتتغير اللون الكوني
+
+464
+00:33:36,300 --> 00:33:41,410
+هتظلها colorless او ممكن انها تاخداللون الكلوني
+
+465
+00:33:41,410 --> 00:33:45,270
+لون ال media طبعا ممكن انها تكون colorless او ممكن
+
+466
+00:33:45,270 --> 00:33:48,250
+تاخد لون ال media لون ال media لون violet فاتح
+
+467
+00:33:48,250 --> 00:33:52,250
+فابدى افرق ما بين ال lactose و ال non lactose خاصة
+
+468
+00:33:52,250 --> 00:33:55,610
+انه ال lactose في المنتر بتكون dark purple لكن هاي
+
+469
+00:33:55,610 --> 00:34:00,070
+لو اخدت لون ال media بيكون لون violet فاتح طيب لو
+
+470
+00:34:00,070 --> 00:34:04,450
+طبق AMP لكن مالاقيت فيه اي نمو يبقى بعرف انه هي
+
+471
+00:34:04,450 --> 00:34:07,830
+high gram positive bacteria صار لها inhibition
+
+472
+00:34:08,070 --> 00:34:12,570
+بواسطة الصبغات الأيوزين والمثلين قبله هك اتعرفنا
+
+473
+00:34:12,570 --> 00:34:24,190
+او اتعرفنا على ال ال EMB نيجي لل slides EMB is a
+
+474
+00:34:24,190 --> 00:34:28,830
+primary differential media هي تعتبر differential
+
+475
+00:34:28,830 --> 00:34:32,390
+media وعرفنا لأن فيها ال lactose ك differential
+
+476
+00:34:32,390 --> 00:34:36,580
+agent however it does inhibit the growth ofsome of
+
+477
+00:34:36,580 --> 00:34:39,700
+a gram positive bacteria و اللي بتعمل inhibition
+
+478
+00:34:39,700 --> 00:34:43,920
+لـ some of a gram positive bacteria تمام فبالتالي
+
+479
+00:34:43,920 --> 00:34:48,560
+هي selection for a gram negative bacteria AMB is
+
+480
+00:34:48,560 --> 00:34:51,380
+used to differentiate between enteric lactose
+
+481
+00:34:51,380 --> 00:34:54,320
+fermenter الكوليفارم and non-lactose fermenter هي
+
+482
+00:34:54,320 --> 00:34:56,660
+بتفرق بين ال lactose fermenter و non-lactose
+
+483
+00:34:56,660 --> 00:34:59,200
+fermenter من عالة ال enterobacteriaceae اللي هي
+
+484
+00:34:59,200 --> 00:35:02,330
+الكوليفارم و ال non-coliformبالإضافة بتعمل
+
+485
+00:35:02,330 --> 00:35:05,850
+identification للـ E.coli لأن الـ E.coli بتعطيها
+
+486
+00:35:05,850 --> 00:35:10,350
+لون مميز لـ Metallic Green Chain فبالتالي بتساعد
+
+487
+00:35:10,350 --> 00:35:14,830
+في ال identification للـ E.coli فده بشكل خاص Iosin
+
+488
+00:35:14,830 --> 00:35:17,550
+Methylene Blue Agar بقولي هذه ال media الـ AMP
+
+489
+00:35:17,550 --> 00:35:22,850
+اللي هي Iosin Methylene Blue Agar content peptone
+
+490
+00:35:22,850 --> 00:35:27,380
+تحتوي علىتحتوي على اللاكتوز طبعا البيبتون كمصدر
+
+491
+00:35:27,380 --> 00:35:30,980
+للتغذية أي ميديا فيها بيبتون كمصدر للتغذية عشان
+
+492
+00:35:30,980 --> 00:35:35,160
+البكتيريا تنمو تتكثر اللاكتوز differential agent
+
+493
+00:35:35,160 --> 00:35:39,140
+الداي الأيوزين والميثاليني بلو تعتبر selective
+
+494
+00:35:39,140 --> 00:35:44,080
+agent واللي ال sugar provide هذا ال sugar هو ال
+
+495
+00:35:44,080 --> 00:35:49,400
+substrate عشان البكتيريا تعملي fermentationThe
+
+496
+00:35:49,400 --> 00:35:51,960
+dyes inhibit the growth of ground positive
+
+497
+00:35:51,960 --> 00:35:54,880
+bacteria organism قولنا إن الصبغات الميسيليني بلو
+
+498
+00:35:54,880 --> 00:35:57,360
+والايوزين بتعمل inhibition لـground positive
+
+499
+00:35:57,360 --> 00:36:02,000
+organism and under acidic condition also produce
+
+500
+00:36:02,000 --> 00:36:08,200
+dark purple بقولّي إذا أتكون عندي acidic product
+
+501
+00:36:08,200 --> 00:36:13,700
+يبقى هاي هتترتبط بالطبع المركب هيرتبط بالايوزين
+
+502
+00:36:13,700 --> 00:36:18,880
+والميسيليني بلو فبالتاليهتعطيني لون dark purple
+
+503
+00:36:18,880 --> 00:36:22,320
+يبقى ال dies بنفس الوقت مع ال under acidic
+
+504
+00:36:22,320 --> 00:36:24,900
+condition مع ال acidic condition لو اتكون عندي
+
+505
+00:36:24,900 --> 00:36:29,400
+acidic product هترتبط مع اللي هي هذا ال acidic
+
+506
+00:36:29,400 --> 00:36:33,740
+product هيرتبط مع الصفغات هيعطيني لون dark purple
+
+507
+00:36:33,740 --> 00:36:37,740
+فبالتالي يعتبر ال dies برضه as BH indicator ك
+
+508
+00:36:37,740 --> 00:36:42,220
+selective agent طيب
+
+509
+00:36:44,060 --> 00:36:48,060
+الأيوزين والميتليني بلو تجعل لكتوز فرمنتر تحصل على
+
+510
+00:36:48,060 --> 00:36:51,220
+لون بنك كولوني بقولي أن الأيوزين والميتليني بلو
+
+511
+00:36:51,220 --> 00:36:55,200
+تجعل لكتوز فرمنتر تحصل على لون بنك كولوني أو هي
+
+512
+00:36:55,200 --> 00:36:59,480
+بالظبط بتاعتي dark purple لش بتاعتي ال dark purple
+
+513
+00:36:59,480 --> 00:37:02,380
+قلنا بتكون أسد ال product هذا ال أسد ال product
+
+514
+00:37:02,380 --> 00:37:06,620
+برتبط مع هذه الصفقات فبعطيني ال بنك or dark purple
+
+515
+00:37:06,620 --> 00:37:11,940
+ال equalize قلنا إنه إلها إشي خاص تمامبقولي هذه
+
+516
+00:37:11,940 --> 00:37:15,300
+الصبغة بتترسب في الخلايا و بتعطي اللون الـ
+
+517
+00:37:15,300 --> 00:37:19,080
+metallic green chain يبقى الـ E.coli بتعطي الـ
+
+518
+00:37:19,080 --> 00:37:24,240
+metallic green chain تمام لكن ال other كل فرم غير
+
+519
+00:37:24,240 --> 00:37:28,080
+ال E.coli بتعطي اللون ال dark purple و عرفنا ليش
+
+520
+00:37:28,080 --> 00:37:30,800
+بتعطي اللون ال metallic green chain لأنه بتكون
+
+521
+00:37:30,800 --> 00:37:33,960
+رابط ما بين اليوزين والميثالين blueطب الـ non
+
+522
+00:37:33,960 --> 00:37:36,740
+-lactose fermenter بقولي هتضلها colorless لأنه ما
+
+523
+00:37:36,740 --> 00:37:39,500
+هيصير fermentation أو ممكن تاخد لون ال media اللي
+
+524
+00:37:39,500 --> 00:37:43,740
+هو اللون ال violet الفاتح زي السلمونيلا بقولي
+
+525
+00:37:43,740 --> 00:37:47,560
+السلمونيلا تعتبر non-lactose fermenter لو نميتها
+
+526
+00:37:47,560 --> 00:37:50,660
+على ال A&B ممكن تكون colorless و ممكن تاخد لون ال
+
+527
+00:37:50,660 --> 00:37:54,800
+media طبعا هي واحدة من أهم المسببات في ال food
+
+528
+00:37:54,800 --> 00:37:57,120
+poisoning في التسمم الغذائي
+
+529
+00:37:59,590 --> 00:38:03,050
+طيب بالنسبة لها هاي ال media used to confirm the
+
+530
+00:38:03,050 --> 00:38:05,670
+presence of E.coli in water sample contaminated
+
+531
+00:38:05,670 --> 00:38:09,830
+with sewage or فيه كالماتيريا ولهاي ال media بشكل
+
+532
+00:38:09,830 --> 00:38:12,550
+خاص مش كمان يتفرق بين ال lactose و ال non-lactose
+
+533
+00:38:12,550 --> 00:38:15,510
+لأنه هنتعرف في ميدية ما كمان يتميز بين ال lactose
+
+534
+00:38:15,510 --> 00:38:19,250
+و ال non-lactose و فعلا بتعطي ألوان واضحة لكن هاي
+
+535
+00:38:19,250 --> 00:38:23,630
+ال media بشكل خاص تستخدم عشان أؤكد وجود ال E.coli
+
+536
+00:38:23,630 --> 00:38:28,190
+في عينات ال water sample contaminated with sewage
+
+537
+00:38:29,020 --> 00:38:33,600
+ليش هاي تستخدم لأن الـ E coli قلنا في هذه ال media
+
+538
+00:38:33,600 --> 00:38:37,140
+ابتعدي لون مميز ابتعدي لون metallic green chain
+
+539
+00:38:37,140 --> 00:38:42,680
+فبالتالي هي بتأكد وجود ال E coli you will use this
+
+540
+00:38:42,680 --> 00:38:46,380
+agar again within water sampling experiment to
+
+541
+00:38:46,380 --> 00:38:49,440
+differentiate between E coli and enterobacter
+
+542
+00:38:49,440 --> 00:38:53,580
+بقوللي في هذه ال media بميز بين ال E coli و ال
+
+543
+00:38:53,580 --> 00:38:58,740
+enterobacter على ال AMP طيب ليش بتميزفي هذه
+
+544
+00:38:58,740 --> 00:39:03,100
+الميديا بين الـ E.coli و الـ Enterobacter الـ E
+
+545
+00:39:03,100 --> 00:39:06,280
+.coli تنتين يعتبروا lactose fermenter لكن الـ E
+
+546
+00:39:06,280 --> 00:39:10,000
+.coli قلنا بينتج acid بيرتبط مع الـ E.osin و
+
+547
+00:39:10,000 --> 00:39:13,660
+الميثيلين blue بتكون رابطة بين الـ E.osin و
+
+548
+00:39:13,660 --> 00:39:16,900
+الميثيلين blue فبيعطيني اللون الـ metallic green
+
+549
+00:39:16,900 --> 00:39:22,500
+الشين بالنسبة للـ Enterobacter هذه اسمها fish eye
+
+550
+00:39:22,500 --> 00:39:27,020
+الـ Enterobacter ملاحظ أن شكلها زي الـ fish eye
+
+551
+00:39:27,810 --> 00:39:32,610
+عارفين بتعمل fermentation لل lactose تمام بتعمل
+
+552
+00:39:32,610 --> 00:39:36,470
+fermentation لل lactose sorry بتعمل fermentation
+
+553
+00:39:36,470 --> 00:39:42,310
+لل lactose بتكون acid هذا ال acid بيرسبت مع الصبغة
+
+554
+00:39:42,310 --> 00:39:48,010
+تمام بيترسب في المنتصف الصبغة بتترسب في المنتصف
+
+555
+00:39:48,520 --> 00:39:52,120
+عالطرف بيكون في عندي colorless فبالتالي بلاحظ أنه
+
+556
+00:39:52,120 --> 00:39:56,200
+هي زي عين السبقة .. زي عين السمكة يبقى السبقة
+
+557
+00:39:56,200 --> 00:40:00,080
+بتترسب في منتصف ال colony على الاتراف بحيث أن ال
+
+558
+00:40:00,080 --> 00:40:04,720
+colony colorless يبقى بتكون زي عين السبقة فش .. فش
+
+559
+00:40:04,720 --> 00:40:09,120
+eye فبالتالي هيك فرقات بين ال equal eye و ال
+
+560
+00:40:09,120 --> 00:40:15,570
+interior بأكترهذا بالنسبة للميديا
+
+561
+00:40:15,570 --> 00:40:17,310
+اللي استخدمه بالتفريق بين الـ E.coli و الـ
+
+562
+00:40:17,310 --> 00:40:20,090
+Enterobacter لأن الـ E.coli بتدّي متاليكا جرينتشين
+
+563
+00:40:20,090 --> 00:40:23,810
+و عرفنا شو السبب الـ Enterobacter بتعطي fish eye
+
+564
+00:40:23,810 --> 00:40:27,550
+لأنه بتعمل fermentation للـ Lactose بيرتبط مع
+
+565
+00:40:27,550 --> 00:40:32,350
+الصبغة بتترثب الصبغة في النص على الطرف بحيث انه في
+
+566
+00:40:32,350 --> 00:40:36,010
+اندي colorless فبالتالي بتكون زي عين الصبغة هيك
+
+567
+00:40:36,010 --> 00:40:37,550
+بنكون خلصنا نيجي ل
+
+568
+00:40:45,100 --> 00:40:50,340
+لسلايدات للصور هان ملاحظ طبق a and b نمت عليه
+
+569
+00:40:50,340 --> 00:40:54,160
+البكتيريا يبقى هي إجرام negative بكتيريا تمام ظهر
+
+570
+00:40:54,160 --> 00:40:56,880
+اللون الأخضر يبقى بحكي أنه والله هي احتمال انها
+
+571
+00:40:56,880 --> 00:41:01,020
+تكون E.coli وعرفنا ليش اتكون اللون الأخضر بالنسبة
+
+572
+00:41:01,020 --> 00:41:07,140
+لهان fish eye colony ملاحظ انهالصبغة اترسبت في
+
+573
+00:41:07,140 --> 00:41:09,660
+المنتصف وعلى الطرف فى عندي colorless يبقى هي
+
+574
+00:41:09,660 --> 00:41:13,580
+احتمال انها تكون enterobacter السبب انه صار فى
+
+575
+00:41:13,580 --> 00:41:17,400
+عندي fermentation للكتاز اتكون acid ارتبط مع
+
+576
+00:41:17,400 --> 00:41:22,480
+الصبغة ارتبط مع الصبغة فبالتالي كونلي لون dark
+
+577
+00:41:22,480 --> 00:41:26,100
+purple اترسبت الصبغة في المنتصف وعلى الطرف كان فى
+
+578
+00:41:26,100 --> 00:41:30,010
+عندي colorless يبقى هى enterobacterطبعاً باقي اللي
+
+579
+00:41:30,010 --> 00:41:33,950
+هي الكليبسيلة والستروبكتر بتعطي لون dark زي هذا
+
+580
+00:41:33,950 --> 00:41:38,070
+لكن ما بتترسف بالمنتصف الكليبسيلة والستروبكتر
+
+581
+00:41:38,070 --> 00:41:43,230
+بروتياس وسلمونيلا نفس الاشي بروتياس وسلمونيلا non
+
+582
+00:41:43,230 --> 00:41:46,730
+-lactose fermenter فبالتالي مش هيتغير اللون
+
+583
+00:41:46,730 --> 00:41:51,270
+الكولوني هتظلها لونها colorless او ممكن انها تاخد
+
+584
+00:41:51,270 --> 00:41:54,030
+اللون ال media اللي هو اللون اللي هو ال violet
+
+585
+00:41:54,030 --> 00:41:58,530
+الفاتحبالنسبة لـ Staphylococcus aureus لـ
+
+586
+00:41:58,530 --> 00:42:02,510
+Staphylococcus aureus على طبق الـ AMP المفترض ما
+
+587
+00:42:02,510 --> 00:42:05,050
+إنها تنمو لأنه هي gram positive بكتيريا يبقى لو
+
+588
+00:42:05,050 --> 00:42:08,830
+جبنا في الامتحان AMP ولاحظنا إنه والله في عندي طبق
+
+589
+00:42:08,830 --> 00:42:13,650
+ما في عندي أي نمو على الطبق يبقى بحكي إنه هاي ال
+
+590
+00:42:13,650 --> 00:42:17,670
+bacteria صار لها inhibition بسبواسطة اللي هي
+
+591
+00:42:17,670 --> 00:42:20,690
+selective agent اليوزينوي الميتليني بلو يبقى
+
+592
+00:42:20,690 --> 00:42:24,570
+احتمال إنها تكون gram positive بكتيرياهذا بالنسبة
+
+593
+00:42:24,570 --> 00:42:31,150
+لـ ال .. اللي هو ال .. شو تفسير الألوان طبعا الـ
+
+594
+00:42:31,150 --> 00:42:35,010
+AMP الـ lactose fermenter لون dark purple لكن الـ
+
+595
+00:42:35,010 --> 00:42:38,530
+E.coli فقط الـ E.coli بتعطي metallic green chain
+
+596
+00:42:38,530 --> 00:42:42,030
+الـ interior بكتر بمعيزها الـ fish eye colony الـ
+
+597
+00:42:42,030 --> 00:42:44,610
+non lactose fermenter بتضلها colorless أو ممكن
+
+598
+00:42:44,610 --> 00:42:49,410
+تاخد لون ال media لون ال .. الوسط أي إجراء
+
+599
+00:42:49,410 --> 00:42:52,540
+positive بكتيريا مش هتنمو على هذا الوسطالـ E.coli
+
+600
+00:42:52,540 --> 00:42:56,040
+من اللاحظ أنه والله هان هي lactose fermented
+
+601
+00:42:56,040 --> 00:43:01,580
+وعرفنا شو سبب تكون اللون الأخضر على ال A&B لـ E
+
+602
+00:43:01,580 --> 00:43:07,840
+.coli هان الـ Enterobacter باخد اللون ال pink تمام
+
+603
+00:43:07,840 --> 00:43:13,660
+على ال A&B احنا قلنا pink or dark purple يبقى هان
+
+604
+00:43:13,660 --> 00:43:18,050
+من اللاحظ أنه احنا مش هنشوفطبعا ال fish eye colony
+
+605
+00:43:18,050 --> 00:43:22,390
+انترو باكتر المفترض انها تكون fish eye colony ليش
+
+606
+00:43:22,390 --> 00:43:26,070
+مش هنشوف هاي ال fish eye colony لأنه هاي مش
+
+607
+00:43:26,070 --> 00:43:29,470
+isolated colony ما زلعت بطريقة الأربع عشان أحصل
+
+608
+00:43:29,470 --> 00:43:32,990
+على isolated colony فبالتالي مش هشوف ال fish eye
+
+609
+00:43:32,990 --> 00:43:35,390
+colony عشان أشوف ال fish eye colony لازم أزرع
+
+610
+00:43:35,390 --> 00:43:41,590
+بطريقة الأربع طيب هيك خلصنا ال A&B نيجي لآخر media
+
+611
+00:43:41,590 --> 00:43:46,120
+اللي هي الماكمكي أجارالمكونكي زي الـ «AMP»
+
+612
+00:43:46,120 --> 00:43:48,860
+«Selection» لـ «Gram Negative Bacteria» يبقى لـ
+
+613
+00:43:48,860 --> 00:43:52,180
+«Gram Positive Bacteria» هيصير لها Inhibition مين
+
+614
+00:43:52,180 --> 00:43:55,460
+الـ «Selective Agent»؟ الـ «Selective Agent» نفس
+
+615
+00:43:55,460 --> 00:44:00,400
+هذه الميديا الـ «AMP» برضه يعتبر «Dyes» لكن بيختلف
+
+616
+00:44:00,400 --> 00:44:03,880
+«Dyes» و «Salt» «Crystal Violet» and «Bi Salt»
+
+617
+00:44:03,880 --> 00:44:08,680
+بيتفق مع ال «AMP» أنه والله «Salt» مع ال «AMP»
+
+618
+00:44:08,680 --> 00:44:12,710
+أنها «Dyes»لكن هناك كان المثلين blue والايوزين
+
+619
+00:44:12,710 --> 00:44:17,230
+والايوزين كانت هناك لكن hand ل crystal violet
+
+620
+00:44:17,230 --> 00:44:21,330
+تعتبر ال by salt يعتبر hand salt يبقى في هذه
+
+621
+00:44:21,330 --> 00:44:24,330
+الميديا selective agent dies and salt crystal
+
+622
+00:44:24,330 --> 00:44:27,770
+violet dies وال by salt يعتبر salt طيب
+
+623
+00:44:27,770 --> 00:44:30,450
+differential agent نفس ال A and B differential
+
+624
+00:44:30,450 --> 00:44:34,030
+agent في هذه الميديا lactose لكن في ال manitol
+
+625
+00:44:34,030 --> 00:44:39,520
+salt أجار كان manitolهتميز ليه نفس الشيباق بين الـ
+
+626
+00:44:39,520 --> 00:44:42,480
+coliform و الـ non-coliform بكتيريا هذه صح بتميز
+
+627
+00:44:42,480 --> 00:44:45,340
+بين الـ coliform و الـ non-coliform لكن بشكل خاص
+
+628
+00:44:45,340 --> 00:44:49,300
+بتميز الـ E-coli تعملي identification للـ E-coli و
+
+629
+00:44:49,300 --> 00:44:53,640
+كمان للـ enterobacter لأنه بتدي فش I كله الـ pH
+
+630
+00:44:53,640 --> 00:44:58,520
+indicator الـ neutral red هذا جديد ممر معناه الـ
+
+631
+00:44:58,520 --> 00:45:01,880
+neutral red نفس الـ methyl red اللي أخدناه تبحث
+
+632
+00:45:01,880 --> 00:45:06,790
+الـ methyl redعكس البيئة اللي فيه نار رد في الاسد
+
+633
+00:45:06,790 --> 00:45:10,190
+بيكون لونه رد وفي الباس بيكون لونه يلو لـ Gram
+
+634
+00:45:10,190 --> 00:45:13,510
+Negative بكتيريا فقط اللي هي هتميز في هذه الميديا
+
+635
+00:45:13,510 --> 00:45:16,770
+هتميز لبعض الـ Lactose Fermenter و لبعض الـ Non
+
+636
+00:45:16,770 --> 00:45:20,010
+Lactose Fermenter اللاكتوز فرمنتر هتعمل
+
+637
+00:45:20,010 --> 00:45:23,190
+fermentation لللاكتوز هيتكوّن اسد بما أنه فيه ND
+
+638
+00:45:23,190 --> 00:45:27,310
+BH Indicator هيعمل على خفض ال BH هيتكوّن لون pink
+
+639
+00:45:27,310 --> 00:45:30,720
+to red كله لكن الـ Non Lactose Fermenterمش هتعمل
+
+640
+00:45:30,720 --> 00:45:33,240
+fermentation للـ lactose هيدلوا لون الـ colony
+
+641
+00:45:33,240 --> 00:45:37,120
+colorless لـ Gram-positive بكتيريا مش هيصيرلها أي
+
+642
+00:45:37,120 --> 00:45:43,780
+نمو زي الـ AMP فبالتالي مش هتنمو على الطبق المكون
+
+643
+00:45:43,780 --> 00:45:47,020
+K agar used to isolate Gram-negative بكتيريا قلنا
+
+644
+00:45:47,020 --> 00:45:49,960
+لأنه هي بتنمي لـ Gram-negative بكتيريا هي تعتبر
+
+645
+00:45:49,960 --> 00:45:53,540
+selective و differential media less selective لأنه
+
+646
+00:45:53,540 --> 00:45:56,620
+لـ Gram-positive بكتيريا بيصيرلها Inhibition من
+
+647
+00:45:56,620 --> 00:45:59,560
+الـ Selective agent الـ bisalt and crystal violet
+
+648
+00:46:00,070 --> 00:46:03,250
+طيب الـ Gram Negative بكتيريا فقط اللي هي اللي
+
+649
+00:46:03,250 --> 00:46:06,690
+هتنمو طيب ديفرانشيات ليش هي differential media
+
+650
+00:46:06,690 --> 00:46:10,190
+لأنها بتفرق بين الـ Gram Negative بكتيريا وقدرتها
+
+651
+00:46:10,190 --> 00:46:14,410
+إنها تعمل fermentation للـ Lactose بالنسبة للـ
+
+652
+00:46:14,410 --> 00:46:16,770
+Coliform طبعا هي بتميز الـ Coliform و الـ Non
+
+653
+00:46:16,770 --> 00:46:19,910
+-coliform نيجي للـ Coliform بسيلاي بتكون acid
+
+654
+00:46:19,910 --> 00:46:23,910
+نتيجة الـ Lactose fermentation فبالتالي بتخلي لون
+
+655
+00:46:23,910 --> 00:46:29,660
+الكولوني يتحول للون أحمر نتيجة خفض الـ pHبقولي هنا
+
+656
+00:46:29,660 --> 00:46:33,700
+في الـ E.coli طبعا تعتبر من الكوليفارم بكتيريا زي
+
+657
+00:46:33,700 --> 00:46:36,500
+ما ال E.coli كانت في ال A وB كانت مميزة باللون
+
+658
+00:46:36,500 --> 00:46:41,500
+الأخضر هنا برضه مميزة في المقنكي بقولي بتنتج
+
+659
+00:46:41,500 --> 00:46:46,940
+produce even greater quantities of acid causing
+
+660
+00:46:46,940 --> 00:46:50,260
+the surrounding media and the colonists to turn
+
+661
+00:46:50,260 --> 00:46:56,120
+red بقولي بتنتج كمية كبيرة من الأسد فبالتاليحوالين
+
+662
+00:46:56,120 --> 00:46:59,120
+اللي هو الـ Media الـ Colony نفسها والـ Media
+
+663
+00:46:59,120 --> 00:47:02,900
+بيصير لونها أحمر تمام هذا اللي بيميز الـ E.coli
+
+664
+00:47:02,900 --> 00:47:07,240
+أنه بلاحظ مش بس الـ Surrounding Media كمان الـ
+
+665
+00:47:07,240 --> 00:47:12,520
+Colony اتحول لونها للون أحمر نتيجة اللي هو الكمية
+
+666
+00:47:12,520 --> 00:47:15,680
+الكبيرة من الأسد اللي نتج من عملية ال fermentation
+
+667
+00:47:15,680 --> 00:47:19,740
+يبقى هذا بيميز الـ E.coli في المكونكي في الـ AMP
+
+668
+00:47:19,740 --> 00:47:23,570
+بتحول اللون للون أخضرالـ Non-Coliform Basilaria أو
+
+669
+00:47:23,570 --> 00:47:26,030
+الـ Non-Lactose Fermenter ما بيصير fermentation
+
+670
+00:47:26,030 --> 00:47:29,950
+للـ Lactose فبالتالي بتظلها transparent or
+
+671
+00:47:29,950 --> 00:47:34,610
+colorless لـ Gram Positive بكتيريا اتفقنا أي ميديا
+
+672
+00:47:34,610 --> 00:47:38,030
+بتعمل inhibition لأي نوع من أنواع البكتيريا ما
+
+673
+00:47:38,030 --> 00:47:41,510
+بيصير kill ما بتموت في هذه الميديا فقط بيصير لـ
+
+674
+00:47:41,510 --> 00:47:48,560
+growth inhibitionطيب نيجي للصور طبق مقنكي لاحظنا
+
+675
+00:47:48,560 --> 00:47:51,280
+فيه انه يبقى الـBacteria Gram Negative Bacteria طب
+
+676
+00:47:51,280 --> 00:47:54,040
+بدني أميز هل هي lactose fermenter or non-lactose
+
+677
+00:47:54,040 --> 00:47:59,840
+fermenter الـ E.coli قولنا إنه بتحول لون الوسط
+
+678
+00:47:59,840 --> 00:48:03,540
+وكمان الكلوني للون الزهر نتيجة إنتاج كمية كبيرة من
+
+679
+00:48:03,540 --> 00:48:08,000
+الأسد فبالتالي عمل على حفظ الـ pH وتحول لون الوسط
+
+680
+00:48:08,000 --> 00:48:14,450
+للون اللي هو أحمر هذا بالنسبة لـ E.coli تمامالـ
+
+681
+00:48:14,450 --> 00:48:17,210
+Enterobacter نفس الـ E.coli الـ E.coli و الـ
+
+682
+00:48:17,210 --> 00:48:19,090
+Enterobacter و الـ Klebsiella و الـ Citrobacter
+
+683
+00:48:19,090 --> 00:48:21,890
+نفس الإيش بتاعته لون pink to red لكن اللي بيفرق
+
+684
+00:48:21,890 --> 00:48:25,310
+الـ E.coli إنها من لاحظ الـ surrounding media و
+
+685
+00:48:25,310 --> 00:48:30,350
+الكولوني لونها أحمر تمام بالنسبة للـ Enterobacter
+
+686
+00:48:30,350 --> 00:48:36,330
+برضه نمت لاحظت أن اللون pink to red يبقى من عائلة
+
+687
+00:48:36,330 --> 00:48:40,910
+الـ coliform لكتوز أو نحكي لكتوز fermented
+
+688
+00:48:40,910 --> 00:48:45,450
+برودياسوي السلمونيلاتنتهى الـ Non-lactose
+
+689
+00:48:45,450 --> 00:48:47,870
+fermentation يبقى مش هتعمل fermentation للاكتوز
+
+690
+00:48:47,870 --> 00:48:51,990
+هتظهر اللون الكلوني لونها colorless or transparent
+
+691
+00:48:51,990 --> 00:48:55,330
+الاستفيلة كوكس أورياسمين لـ Gram-positive bacteria
+
+692
+00:48:55,330 --> 00:48:58,570
+فبالتالي مش هلاقي أي نمو على الطواق لأن هاي ال
+
+693
+00:48:58,570 --> 00:49:01,970
+media بيصير فيها inhibition لـ Gram-positive
+
+694
+00:49:01,970 --> 00:49:07,210
+bacteria طيب لو سألنا طبعا هان هي معنى ماد نتيجة
+
+695
+00:49:07,210 --> 00:49:11,170
+انه صار Inhibition بواسطة ال crystal violet و ال
+
+696
+00:49:11,170 --> 00:49:18,670
+bile saltلو سألنا سؤال إن الصدوموناس الصدوموناس
+
+697
+00:49:18,670 --> 00:49:22,470
+على طبق الـ AMP أو طبق المكونكي طبعاً هي الـ Gram
+
+698
+00:49:22,470 --> 00:49:26,350
+Negative Bacteria فأكيد الصدوموناس هتنمو على الـ
+
+699
+00:49:26,350 --> 00:49:30,130
+AMP والمكونكي وإحنا قلنا تنتين مش بس للـ
+
+700
+00:49:30,130 --> 00:49:32,610
+Enterobacteriasis هي بتنمي كل Gram Negative
+
+701
+00:49:32,610 --> 00:49:36,090
+Bacteria فعشان هيك لازم أعمل فحوصات فحوصات الـ
+
+702
+00:49:36,090 --> 00:49:39,340
+Oxidase فحوصات الـ Gelicose Fermentationعشان اتأكد
+
+703
+00:49:39,340 --> 00:49:42,400
+ان هي من عائلة الانتيرو بكتيريازي وبعدين احكي هل
+
+704
+00:49:42,400 --> 00:49:45,700
+هي من عائلة الكوليفارم او من الـnon-coliform
+
+705
+00:49:45,700 --> 00:49:49,600
+السوداموناس تعتبر اجرام نجатив بكتيريا اكيد هتنمو
+
+706
+00:49:49,600 --> 00:49:55,140
+على طبق المكونكي او ال AMP طيب ايش هتعطي كولوني؟
+
+707
+00:49:55,140 --> 00:49:58,640
+السوداموناس بالاصل هي non-gelicose fermenter
+
+708
+00:49:58,640 --> 00:50:02,800
+فبالتالي هي non-lactose بما انه ماعملة جلد
+
+709
+00:50:02,800 --> 00:50:05,540
+fermentation لأبسط سكر فبالتالي مش هتعمل
+
+710
+00:50:05,540 --> 00:50:10,240
+fermentation لالـ Dysaccharide اللاكتاز فبالتالي
+
+711
+00:50:10,240 --> 00:50:14,100
+تعتبر non-lactose fermenter يبقى على طبق الميكونكي
+
+712
+00:50:14,100 --> 00:50:18,280
+وعلى طبق ال A&B ال Sodomonas هتعامل معاملة ال non
+
+713
+00:50:18,280 --> 00:50:22,140
+-lactose fermenter هيكون على الميكونكي colorless
+
+714
+00:50:22,140 --> 00:50:25,960
+على ال A&B ممكن انها تكون colorless وممكن تاخد لون
+
+715
+00:50:25,960 --> 00:50:31,400
+الميدى هذا بالنسبة لل Sodomonas فاكما نكون خلصنا
+
+716
+00:50:32,240 --> 00:50:34,460
+معمل الـ «Selective» والـ «Differential Media»
+
+717
+00:50:34,460 --> 00:50:37,740
+تعرفنا على 3 ميديات مكون K, A, M, B مانيتال،
+
+718
+00:50:37,740 --> 00:50:40,220
+asphalt، أجار، كل ميديا من الـ «Selective» من الـ
+
+719
+00:50:40,220 --> 00:50:43,980
+«Differential Agent» أش بتعطي كيف بتفرق بين مين
+
+720
+00:50:43,980 --> 00:50:47,480
+ومين، كل مثلا الـ «Lactose Fermenter»، الـ «Non
+
+721
+00:50:47,480 --> 00:50:51,880
+-Lactose Fermenter»، شو اللون في كل حالة، كذلك في
+
+722
+00:50:51,880 --> 00:50:54,420
+المانيتال، اللي هي المانيتال الـ «Fermenter» والـ
+
+723
+00:50:54,420 --> 00:50:57,560
+«Non-Mannitol Fermenter» في كل منهم أش بتعطي لون
+
+724
+00:50:58,440 --> 00:51:03,920
+بأتمنى الأمور والشرح يكون واضح يعطيكم العافية
+
+725
+00:51:03,920 --> 00:51:06,540
+والسلام عليكم ورحمة الله وبركاته
+
diff --git a/PL9fwy3NUQKwZWgAb_V1ATlRRsH-6i3cPC/WtgYOo5DhhI_raw.json b/PL9fwy3NUQKwZWgAb_V1ATlRRsH-6i3cPC/WtgYOo5DhhI_raw.json
new file mode 100644
index 0000000000000000000000000000000000000000..fca9eaaedc7aaf0c596919fe995125ce773a7422
--- /dev/null
+++ b/PL9fwy3NUQKwZWgAb_V1ATlRRsH-6i3cPC/WtgYOo5DhhI_raw.json
@@ -0,0 +1 @@
+{"segments": [{"id": 1, "seek": 3100, "start": 2.06, "end": 31.0, "text": "السلام عليكم ورحمة الله وبركاته يعطيكم العافية جميعًا اليوم المحاضرة بانوان Selective and Differential Media في المحاضرة أو في المعامل السابقة اتكلمنا عن الـ Media عرفنا الـ Media وصنفنا الـ Media أو قسمنا الـ Media حسب الـ Function حسب الـ Consistency حسب الـ Nutrient وذكرنا عدة تصطيفات تحت كل صنف من هذه الأصناف", "tokens": [6027, 3794, 37440, 25894, 24793, 4032, 2288, 35571, 3660, 21984, 4032, 26890, 4117, 9307, 3224, 7251, 3615, 9566, 1829, 24793, 18863, 31845, 10632, 10874, 2304, 40228, 14111, 995, 45595, 20498, 9673, 5016, 46958, 25720, 4724, 7649, 2407, 7649, 13638, 488, 293, 413, 12612, 2549, 14741, 8978, 9673, 5016, 46958, 25720, 34051, 8978, 9673, 3615, 10943, 1211, 21136, 16758, 28671, 1975, 2655, 4117, 19528, 8315, 18871, 2423, 39184, 14741, 6225, 28480, 8315, 2423, 39184, 14741, 4032, 9381, 1863, 5172, 8315, 2423, 39184, 14741, 34051, 12174, 38251, 8315, 2423, 39184, 14741, 11331, 35457, 2423, 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احنا قلنا او مرمعنا قبل كده ان ال microbiologist بيختار ال media حسب نوع العينة بكون انا عارفة ان والله مثلا عينة ال urine ال most common", "tokens": [6027, 39184, 5818, 20027, 9201, 4724, 1829, 46456, 9640, 2423, 3021, 34051, 4724, 1829, 46456, 9640, 3021, 16490, 33546, 3660, 6225, 8592, 7649, 37495, 11622, 1211, 14739, 14407, 3615, 20449, 9957, 3660, 9154, 9673, 1829, 4117, 32887, 3555, 9307, 4032, 1829, 25957, 1211, 37279, 16572, 5172, 5296, 28814, 1211, 11296, 1975, 5016, 8315, 12174, 1211, 8315, 1975, 2407, 3714, 2288, 2304, 3615, 8315, 12174, 36150, 9122, 3215, 3224, 16472, 2423, 4532, 5614, 9201, 4724, 1829, 46456, 9640, 2423, 3021, 11331, 35457, 8717, 45367, 18863, 9957, 3660, 4724, 30544, 1975, 8315, 6225, 9640, 5172, 3660, 16472, 16070, 43761, 50113, 15040, 6225, 9957, 3660, 2423, 27638, 2423, 881, 2689], "avg_logprob": -0.13302951747620548, "compression_ratio": 1.7818181818181817, "no_speech_prob": 1.430511474609375e-06, "words": [{"start": 168.75, "end": 169.21, "word": 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هنمعرف شو هي ال selective media وانا ذكرت التعريف في بداية المعمل allow certain type of organism to grow بدها تسمح لنوع معين فقط انه ينمو and inhibit the growth of other organism وباقي ال organism هتعمله inhibition مش هتعمله kill", "tokens": [2655, 14851, 9778, 40448, 5296, 3615, 11622, 1211, 8717, 45367, 20449, 9957, 9154, 9673, 1829, 4117, 32887, 3555, 9307, 34683, 3615, 16572, 5172, 11296, 33930, 3021, 8032, 1863, 2304, 3615, 28480, 13412, 2407, 39896, 2423, 33930, 3021, 4032, 7649, 995, 29910, 37983, 2655, 16712, 3615, 16572, 5172, 8978, 4724, 28259, 10632, 9673, 25957, 1211, 2089, 1629, 2010, 295, 24128, 281, 1852, 47525, 11296, 6055, 38251, 5016, 5296, 1863, 45367, 20449, 9957, 6156, 47432, 16472, 3224, 7251, 1863, 2304, 2407, 293, 49858, 264, 4599, 295, 661, 24128, 4032, 3555, 995, 38436, 2423, 24128, 8032, 2655, 25957, 43761, 20406, 849, 37893, 8032, 2655, 25957, 43761, 1961], "avg_logprob": -0.13020833560398648, "compression_ratio": 1.6857142857142857, "no_speech_prob": 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907.81, "end": 925.57, "text": "that is that are able to select to select against the growth of certain organism بقوللي هي بتسمح انها لنمو نوع معين من الميكروبات او بتنمي نوع معين من الميكروبات while the organism that do grow my eggs", "tokens": [6780, 307, 300, 366, 1075, 281, 3048, 281, 3048, 1970, 264, 4599, 295, 1629, 24128, 4724, 39648, 20292, 39896, 39894, 38251, 5016, 16472, 11296, 5296, 1863, 2304, 2407, 8717, 45367, 20449, 9957, 9154, 9673, 1829, 4117, 32887, 3555, 9307, 1975, 2407, 39894, 1863, 2304, 1829, 8717, 45367, 20449, 9957, 9154, 9673, 1829, 4117, 32887, 3555, 9307, 1339, 264, 24128, 300, 360, 1852, 452, 6466], "avg_logprob": -0.23557692307692307, "compression_ratio": 1.598802395209581, "no_speech_prob": 2.384185791015625e-07, "words": [{"start": 907.81, "end": 908.19, "word": "that", "probability": 0.263671875}, {"start": 908.19, "end": 908.45, "word": " is", "probability": 0.7783203125}, {"start": 908.45, "end": 908.75, "word": " that", "probability": 0.8994140625}, 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هنا", "probability": 0.329345703125}, {"start": 954.68, "end": 955.08, "word": " في", "probability": 0.9677734375}, {"start": 955.08, "end": 955.36, "word": " هذا", "probability": 0.93701171875}, {"start": 955.36, "end": 955.84, "word": " الشابط", "probability": 0.385284423828125}, {"start": 955.84, "end": 956.32, "word": " اذاكر", "probability": 0.54803466796875}], "temperature": 1.0}, {"id": 37, "seek": 98282, "start": 956.68, "end": 982.82, "text": "تلت أمثلة على selective و differential media أول media هنتعرف عليها اللي هي ال money tall, salt, agar بس هان في خطأ كتابي او هو ليس I money tall, salt, agar هذه ال media هي selection of استفيل ككة", "tokens": [2655, 1211, 2655, 5551, 2304, 12984, 37977, 15844, 33930, 4032, 15756, 3021, 5551, 12610, 3021, 8032, 29399, 3615, 28480, 25894, 11296, 13672, 1829, 39896, 2423, 1460, 6764, 11, 5139, 11, 623, 289, 4724, 3794, 8032, 7649, 8978, 16490, 9566, 10721, 9122, 2655, 16758, 1829, 1975, 2407, 31439, 32239, 3794, 286, 1460, 6764, 11, 5139, 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"end": 1255.51, "text": "يبقى لون الوسط لون الـmedia لونها زهري هلاقي ان الـmedia تحول لونها لللون الأصفر وممكن إذا اتكون كمية كبيرة من الأسد ألاقي الـcolony نفسها تحولت للون الأصفر من الأمثلة عليها الاستافيلوكوكا او الاسم طيب لو مسكت الطبق ولاقيت والله عندي كولوني لونها colorless لكن الطبق حوالينه ضاله لونه pink يبقى بعرف ان البكتيريا استافيلوكوكاي لأن الـmedia selection of استافيلوكوكا", "tokens": [1829, 3555, 4587, 7578, 5296, 11536, 2423, 41779, 9566, 5296, 11536, 2423, 39184, 1912, 654, 5296, 11536, 11296, 30767, 3224, 16572, 8032, 15040, 38436, 16472, 2423, 39184, 1912, 654, 6055, 5016, 12610, 5296, 11536, 11296, 24976, 1211, 11536, 16247, 9381, 5172, 2288, 4032, 2304, 43020, 11933, 15730, 1975, 2655, 30544, 9122, 2304, 10632, 9122, 3555, 48923, 9154, 16247, 3794, 3215, 5551, 15040, 38436, 2423, 39184, 8768, 2526, 8717, 36178, 11296, 6055, 5016, 12610, 2655, 5296, 1211, 11536, 16247, 9381, 5172, 2288, 9154, 16247, 2304, 12984, 37977, 25894, 11296, 42963, 14851, 31845, 26895, 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"word": " growth", "probability": 0.59228515625}, {"start": 1450.36, "end": 1450.66, "word": " of", "probability": 0.6875}, {"start": 1450.66, "end": 1451.04, "word": " own", "probability": 0.822265625}, {"start": 1451.04, "end": 1451.46, "word": " manitol", "probability": 0.80419921875}, {"start": 1451.46, "end": 1452.42, "word": " نمسح", "probability": 0.9581298828125}, {"start": 1452.42, "end": 1452.68, "word": " هذه", "probability": 0.67626953125}, {"start": 1452.68, "end": 1453.24, "word": " الجملة", "probability": 0.93115234375}, {"start": 1453.24, "end": 1457.68, "word": " ال", "probability": 0.54638671875}, {"start": 1457.68, "end": 1458.76, "word": " micrococcus", "probability": 0.948486328125}, {"start": 1458.76, "end": 1459.12, "word": " بقول", "probability": 0.8408203125}, {"start": 1459.12, "end": 1459.26, "word": " لي", "probability": 0.1192626953125}, {"start": 1459.26, "end": 1459.54, "word": " نفس", "probability": 0.989013671875}, {"start": 1459.54, "end": 1460.32, "word": " الاستفيله", "probability": 0.79453125}, {"start": 1460.32, "end": 1460.8, "word": " coccus", "probability": 0.6036376953125}, {"start": 1460.8, "end": 1461.6, "word": " ابتنموا", "probability": 0.8857421875}, {"start": 1461.6, "end": 1461.9, "word": " على", "probability": 0.87548828125}, {"start": 1461.9, "end": 1462.82, "word": " ال", "probability": 0.94091796875}, {"start": 1462.82, "end": 1463.68, "word": " manitol", "probability": 0.8544921875}, {"start": 1463.68, "end": 1464.04, "word": " salt", "probability": 0.8798828125}, {"start": 1464.04, "end": 1464.36, "word": " agar", "probability": 0.806396484375}, {"start": 1464.36, "end": 1464.48, "word": " و", "probability": 0.407470703125}, {"start": 1464.48, "end": 1464.86, "word": " بتعمل", "probability": 0.9186197916666666}, {"start": 1464.86, "end": 1465.54, "word": " fermentation", "probability": 0.98291015625}, {"start": 1465.54, "end": 1466.68, "word": " للمانيتال", "probability": 0.8265625}, {"start": 1466.68, "end": 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the selective media مابدي أثق في ال selective media لازم انا اعمل فحوصات اعمل جرامستين اعمل فحوصات كيميائية هي بتسهل علي التعريف لكن انا", "tokens": [3224, 1829, 13672, 1829, 39894, 1863, 2304, 2407, 15844, 3714, 1829, 16254, 3660, 2423, 39184, 2458, 270, 3298, 259, 5139, 623, 289, 9122, 33911, 44945, 5016, 4117, 1829, 14739, 3224, 2423, 4532, 9122, 4117, 3794, 8717, 36178, 5296, 3794, 3224, 8978, 3224, 9122, 4117, 3794, 257, 540, 301, 39894, 1863, 2304, 2407, 4032, 39894, 3615, 9566, 1829, 13672, 11536, 16247, 9381, 5172, 2288, 47525, 8315, 8717, 3615, 28480, 20449, 1211, 20498, 3660, 1128, 1562, 3361, 307, 294, 3048, 4253, 295, 264, 33930, 3021, 3714, 16758, 16254, 5551, 12984, 4587, 8978, 2423, 33930, 3021, 5296, 31377, 2304, 1975, 8315, 1975, 25957, 1211, 6156, 5016, 2407, 9381, 9307, 1975, 25957, 1211, 10874, 2288, 10943, 14851, 9957, 1975, 25957, 1211, 6156, 5016, 2407, 9381, 9307, 9122, 32640, 1829, 16373, 10632, 39896, 39894, 3794, 3224, 1211, 25894, 16712, 3615, 16572, 5172, 44381, 1975, 8315], "avg_logprob": -0.22650709811677325, "compression_ratio": 1.7321428571428572, "no_speech_prob": 1.1324882507324219e-06, "words": [{"start": 1470.57, "end": 1471.15, "word": "هي", "probability": 0.730224609375}, {"start": 1471.15, "end": 1471.59, "word": " اللي", "probability": 0.7054443359375}, {"start": 1471.59, "end": 1472.27, "word": " بتنمو", "probability": 0.8460693359375}, {"start": 1472.27, "end": 1472.63, "word": " على", "probability": 0.732421875}, {"start": 1472.63, "end": 1473.45, "word": " ميدية", "probability": 0.842529296875}, {"start": 1473.45, "end": 1473.59, "word": " الـ", "probability": 0.658447265625}, {"start": 1473.59, "end": 1474.05, "word": " Manitoulin", "probability": 0.669830322265625}, {"start": 1474.05, "end": 1474.23, "word": " salt", "probability": 0.438232421875}, {"start": 1474.23, "end": 1474.67, "word": " agar", "probability": 0.718505859375}, {"start": 1474.67, "end": 1474.95, "word": " كيف", "probability": 0.9130859375}, 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1487.13, "end": 1487.37, "word": " of", "probability": 0.95556640625}, {"start": 1487.37, "end": 1487.53, "word": " the", "probability": 0.72119140625}, {"start": 1487.53, "end": 1488.03, "word": " selective", "probability": 0.916015625}, {"start": 1488.03, "end": 1488.41, "word": " media", "probability": 0.93798828125}, {"start": 1488.41, "end": 1489.23, "word": " مابدي", "probability": 0.7099609375}, {"start": 1489.23, "end": 1489.67, "word": " أثق", "probability": 0.9440104166666666}, {"start": 1489.67, "end": 1489.91, "word": " في", "probability": 0.9775390625}, {"start": 1489.91, "end": 1490.17, "word": " ال", "probability": 0.7236328125}, {"start": 1490.17, "end": 1490.57, "word": " selective", "probability": 0.85986328125}, {"start": 1490.57, "end": 1491.07, "word": " media", "probability": 0.9677734375}, {"start": 1491.07, "end": 1491.45, "word": " لازم", "probability": 0.9876302083333334}, {"start": 1491.45, "end": 1491.63, "word": " انا", "probability": 0.706787109375}, {"start": 1491.63, "end": 1492.07, "word": " اعمل", "probability": 0.8595377604166666}, {"start": 1492.07, "end": 1492.67, "word": " فحوصات", "probability": 0.97294921875}, {"start": 1492.67, "end": 1492.99, "word": " اعمل", "probability": 0.8194986979166666}, {"start": 1492.99, "end": 1493.67, "word": " جرامستين", "probability": 0.694775390625}, {"start": 1493.67, "end": 1493.97, "word": " اعمل", "probability": 0.9679361979166666}, {"start": 1493.97, "end": 1494.37, "word": " فحوصات", "probability": 0.9958984375}, {"start": 1494.37, "end": 1494.77, "word": " كيميائية", "probability": 0.9619140625}, {"start": 1494.77, "end": 1494.93, "word": " هي", "probability": 0.5986328125}, {"start": 1494.93, "end": 1495.43, "word": " بتسهل", "probability": 0.9862060546875}, {"start": 1495.43, "end": 1495.65, "word": " علي", "probability": 0.7275390625}, {"start": 1495.65, "end": 1496.91, "word": " التعريف", "probability": 0.89404296875}, {"start": 1496.91, "end": 1497.21, "word": " لكن", "probability": 0.93798828125}, {"start": 1497.21, "end": 1497.61, "word": " انا", "probability": 0.892333984375}], "temperature": 1.0}, {"id": 57, "seek": 152643, "start": 1497.89, "end": 1526.43, "text": "مش من حد ما افتح طبق الـ Manitoula Salt Agar أشوف اللون أصفر خلص على طول الـ Staphylococcus Aureus لأ لازم أعمل إجرامستان لازم أمشي فحوصات لازم أمشي كل الفحوصات حتى أوصل لتعريفي نهائي فقط بعرف أنه هي عملت fermentation لالـ Manitoula من ميدية الاستفيلو من ميدية الـ Manitoula Salt Agar يبقى هنا فيه طبعا دراسات حديثة بتأكد أنه ال micrococcus نفس لـ Staphylococcus Aureus على", "tokens": [2304, 8592, 9154, 11331, 3215, 19446, 1975, 5172, 2655, 5016, 23032, 3555, 4587, 2423, 39184, 2458, 270, 263, 875, 19503, 2725, 289, 5551, 8592, 38688, 13672, 11536, 5551, 9381, 5172, 2288, 16490, 1211, 9381, 15844, 23032, 12610, 2423, 39184, 745, 569, 3495, 5842, 905, 1149, 316, 540, 301, 5296, 10721, 5296, 31377, 2304, 5551, 25957, 1211, 11933, 7435, 2288, 10943, 14851, 7649, 5296, 31377, 2304, 5551, 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هذا بالنسبة للـ Lactose Fermenter الـ Non-Lactose Fermenter بقول لي ما بيصير اي fermentation للـ Lactose فبالتالي مش هيتغير اللون ال media ومش هتتغير اللون الكوني هتظلها colorless او ممكن انها تاخد", "tokens": [1211, 19452, 2423, 39184, 462, 13, 8768, 72, 39894, 30544, 12602, 16758, 9566, 3660, 19446, 49374, 33688, 1829, 11622, 9957, 16070, 2304, 1829, 3794, 1829, 20292, 7649, 4724, 1211, 2407, 44381, 8032, 1863, 19446, 39894, 2655, 30544, 12602, 16758, 9566, 3660, 6156, 3555, 29399, 7435, 13672, 11536, 2423, 26890, 36150, 8032, 1863, 39894, 2655, 30544, 12602, 16758, 9566, 3660, 19446, 49374, 33688, 1829, 11622, 9957, 16070, 2304, 1829, 3794, 1829, 20292, 7649, 4724, 1211, 2407, 6156, 3555, 29399, 7435, 13672, 11536, 2423, 39184, 49447, 299, 6969, 1240, 268, 23758, 20666, 1863, 35457, 3660, 24976, 39184, 441, 578, 541, 10728, 518, 260, 2423, 39184, 8774, 12, 43, 578, 541, 10728, 518, 260, 4724, 39648, 32239, 19446, 4724, 1829, 9381, 13546, 1975, 1829, 43161, 24976, 39184, 441, 578, 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{"start": 2115.31, "end": 2115.57, "word": " Blue", "probability": 0.86767578125}, {"start": 2115.57, "end": 2116.03, "word": " Agar", "probability": 0.93408203125}, {"start": 2116.03, "end": 2116.37, "word": " بقولي", "probability": 0.6355794270833334}, {"start": 2116.37, "end": 2116.57, "word": " هذه", "probability": 0.45458984375}, {"start": 2116.57, "end": 2116.73, "word": " ال", "probability": 0.75830078125}, {"start": 2116.73, "end": 2116.95, "word": " media", "probability": 0.7958984375}, {"start": 2116.95, "end": 2117.15, "word": " الـ", "probability": 0.4794921875}, {"start": 2117.15, "end": 2117.55, "word": " AMP", "probability": 0.7470703125}, {"start": 2117.55, "end": 2118.67, "word": " اللي", "probability": 0.899169921875}, {"start": 2118.67, "end": 2119.03, "word": " هي", "probability": 0.8447265625}, {"start": 2119.03, "end": 2119.71, "word": " Iosin", "probability": 0.9832356770833334}, {"start": 2119.71, "end": 2120.15, "word": " Methylene", "probability": 0.852783203125}, {"start": 2120.15, "end": 2120.71, "word": " Blue", "probability": 0.974609375}, {"start": 2120.71, "end": 2121.31, "word": " Agar", "probability": 0.95361328125}, {"start": 2121.31, "end": 2122.03, "word": " content", "probability": 0.26318359375}, {"start": 2122.03, "end": 2122.85, "word": " peptone", "probability": 0.5225016276041666}, {"start": 2122.85, "end": 2123.43, "word": " تحتوي", "probability": 0.83349609375}, {"start": 2123.43, "end": 2123.65, "word": " على", "probability": 0.5283203125}], "temperature": 1.0}, {"id": 80, "seek": 214660, "start": 2124.66, "end": 2146.6, "text": "تحتوي على اللاكتوز طبعا البيبتون كمصدر للتغذية أي ميديا فيها بيبتون كمصدر للتغذية عشان البكتيريا تنمو تتكثر اللاكتوز differential agent الداي الأيوزين والميثاليني بلو تعتبر selective agent واللي ال sugar provide هذا ال sugar هو ال substrate عشان البكتيريا تعملي fermentation", "tokens": [2655, 33753, 45865, 15844, 13672, 995, 4117, 2655, 2407, 11622, 23032, 3555, 3615, 995, 2423, 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selective", "probability": 0.94921875}, {"start": 2139.14, "end": 2140.14, "word": " agent", "probability": 0.92919921875}, {"start": 2140.14, "end": 2140.78, "word": " واللي", "probability": 0.3890380859375}, {"start": 2140.78, "end": 2140.98, "word": " ال", "probability": 0.60888671875}, {"start": 2140.98, "end": 2141.22, "word": " sugar", "probability": 0.7451171875}, {"start": 2141.22, "end": 2142.02, "word": " provide", "probability": 0.833984375}, {"start": 2142.02, "end": 2143.1, "word": " هذا", "probability": 0.8037109375}, {"start": 2143.1, "end": 2143.22, "word": " ال", "probability": 0.767578125}, {"start": 2143.22, "end": 2143.5, "word": " sugar", "probability": 0.888671875}, {"start": 2143.5, "end": 2143.84, "word": " هو", "probability": 0.9794921875}, {"start": 2143.84, "end": 2144.08, "word": " ال", "probability": 0.89111328125}, {"start": 2144.08, "end": 2144.5, "word": " substrate", "probability": 0.87451171875}, {"start": 2144.5, "end": 2144.98, "word": " عشان", "probability": 0.9861653645833334}, {"start": 2144.98, "end": 2145.66, "word": " البكتيريا", "probability": 0.98876953125}, {"start": 2145.66, "end": 2146.08, "word": " تعملي", "probability": 0.8177083333333334}, {"start": 2146.08, "end": 2146.6, "word": " fermentation", "probability": 0.96630859375}], "temperature": 1.0}, {"id": 81, "seek": 217608, "start": 2148.44, "end": 2176.08, "text": "The dyes inhibit the growth of ground positive bacteria organism قولنا إن الصبغات الميسيليني بلو والايوزين بتعمل inhibition لـground positive organism and under acidic condition also produce dark purple بقولّي إذا أتكون عندي acidic product يبقى هاي هتترتبط بالطبع المركب هيرتبط بالايوزين والميسيليني بلو فبالتالي", "tokens": [2278, 274, 2346, 49858, 264, 4599, 295, 2727, 3353, 11763, 24128, 12174, 12610, 8315, 36145, 31767, 3555, 17082, 9307, 9673, 1829, 3794, 26895, 9957, 1829, 4724, 1211, 2407, 16070, 47302, 2407, 11622, 9957, 39894, 25957, 1211, 20406, 849, 5296, 39184, 2921, 3353, 24128, 293, 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"end": 2156.5, "word": " inhibition", "probability": 0.822265625}, {"start": 2156.5, "end": 2156.92, "word": " لـground", "probability": 0.52490234375}, {"start": 2156.92, "end": 2157.36, "word": " positive", "probability": 0.91845703125}, {"start": 2157.36, "end": 2158.12, "word": " organism", "probability": 0.9658203125}, {"start": 2158.12, "end": 2159.04, "word": " and", "probability": 0.5703125}, {"start": 2159.04, "end": 2159.6, "word": " under", "probability": 0.86669921875}, {"start": 2159.6, "end": 2160.06, "word": " acidic", "probability": 0.9521484375}, {"start": 2160.06, "end": 2160.6, "word": " condition", "probability": 0.60546875}, {"start": 2160.6, "end": 2161.08, "word": " also", "probability": 0.6044921875}, {"start": 2161.08, "end": 2162.0, "word": " produce", "probability": 0.7109375}, {"start": 2162.0, "end": 2162.54, "word": " dark", "probability": 0.865234375}, {"start": 2162.54, "end": 2162.84, "word": " purple", "probability": 0.939453125}, {"start": 2162.84, "end": 2163.46, "word": " بقولّي", "probability": 0.64337158203125}, {"start": 2163.46, "end": 2164.04, "word": " إذا", "probability": 0.960205078125}, {"start": 2164.04, "end": 2164.36, "word": " أتكون", "probability": 0.747314453125}, {"start": 2164.36, "end": 2164.7, "word": " عندي", "probability": 0.757568359375}, {"start": 2164.7, "end": 2165.14, "word": " acidic", "probability": 0.6044921875}, {"start": 2165.14, "end": 2168.2, "word": " product", "probability": 0.95947265625}, {"start": 2168.2, "end": 2168.7, "word": " يبقى", "probability": 0.94775390625}, {"start": 2168.7, "end": 2169.08, "word": " هاي", "probability": 0.5654296875}, {"start": 2169.08, "end": 2170.0, "word": " هتترتبط", "probability": 0.9562174479166666}, {"start": 2170.0, "end": 2172.0, "word": " بالطبع", "probability": 0.7149658203125}, {"start": 2172.0, "end": 2172.58, "word": " المركب", "probability": 0.7994791666666666}, {"start": 2172.58, "end": 2173.1, "word": " هيرتبط", "probability": 0.90263671875}, {"start": 2173.1, "end": 2173.7, "word": " بالايوزين", "probability": 0.9203125}, {"start": 2173.7, "end": 2174.4, "word": " والميسيليني", "probability": 0.931640625}, {"start": 2174.4, "end": 2174.74, "word": " بلو", "probability": 0.8650716145833334}, {"start": 2174.74, "end": 2176.08, "word": " فبالتالي", "probability": 0.9783528645833334}], "temperature": 1.0}, {"id": 82, "seek": 220221, "start": 2176.52, "end": 2202.22, "text": "هتعطيني لون dark purple يبقى ال dies بنفس الوقت مع ال under acidic condition مع ال acidic condition لو اتكون عندي acidic product هترتبط مع اللي هي هذا ال acidic product هيرتبط مع الصفغات هيعطيني لون dark purple فبالتالي يعتبر ال dies برضه as BH indicator ك selective agent طيب", "tokens": [3224, 2655, 3615, 9566, 1829, 22653, 5296, 11536, 2877, 9656, 7251, 3555, 4587, 7578, 2423, 2714, 44945, 36178, 2423, 30543, 2655, 20449, 2423, 833, 39514, 4188, 20449, 2423, 39514, 4188, 45164, 1975, 2655, 30544, 18871, 16254, 39514, 1674, 8032, 2655, 43500, 3555, 9566, 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2388.01, "text": "عارفين بتعمل fermentation لل lactose تمام بتعمل fermentation لل lactose sorry بتعمل fermentation لل lactose بتكون acid هذا ال acid بيرسبت مع الصبغة تمام بيترسب في المنتصف الصبغة بتترسب في المنتصف", "tokens": [3615, 9640, 5172, 9957, 39894, 25957, 1211, 43161, 24976, 34042, 541, 46811, 10943, 39894, 25957, 1211, 43161, 24976, 34042, 541, 2597, 39894, 25957, 1211, 43161, 24976, 34042, 541, 39894, 30544, 8258, 23758, 2423, 8258, 4724, 13546, 35457, 2655, 20449, 31767, 3555, 17082, 3660, 46811, 10943, 4724, 36081, 2288, 35457, 8978, 9673, 29399, 9381, 5172, 31767, 3555, 17082, 3660, 39894, 2655, 2288, 35457, 8978, 9673, 29399, 9381, 5172], "avg_logprob": -0.15498621696058443, "compression_ratio": 2.0, "no_speech_prob": 8.940696716308594e-07, "words": [{"start": 2367.81, "end": 2368.41, "word": "عارفين", "probability": 0.913818359375}, {"start": 2368.41, "end": 2368.91, "word": " بتعمل", "probability": 0.8924153645833334}, {"start": 2368.91, "end": 2369.41, "word": " 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{"start": 3054.42, "end": 3054.52, "word": " «Non", "probability": 0.926513671875}, {"start": 3054.52, "end": 3054.88, "word": "-Mannitol", "probability": 0.645904541015625}, {"start": 3054.88, "end": 3055.38, "word": " Fermenter»", "probability": 0.84808349609375}, {"start": 3055.38, "end": 3055.78, "word": " في", "probability": 0.74560546875}, {"start": 3055.78, "end": 3056.04, "word": " كل", "probability": 0.98974609375}, {"start": 3056.04, "end": 3056.34, "word": " منهم", "probability": 0.989990234375}, {"start": 3056.34, "end": 3056.66, "word": " أش", "probability": 0.849609375}, {"start": 3056.66, "end": 3057.26, "word": " بتعطي", "probability": 0.80902099609375}, {"start": 3057.26, "end": 3057.56, "word": " لون", "probability": 0.772705078125}], "temperature": 1.0}, {"id": 115, "seek": 306654, "start": 3058.44, "end": 3066.54, "text": "بأتمنى الأمور والشرح يكون واضح يعطيكم العافية والسلام عليكم ورحمة الله وبركاته", "tokens": [3555, 10721, 2655, 27842, 7578, 16247, 2304, 13063, 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\ No newline at end of file
diff --git a/PL9fwy3NUQKwZWgAb_V1ATlRRsH-6i3cPC/WtgYOo5DhhI_raw.srt b/PL9fwy3NUQKwZWgAb_V1ATlRRsH-6i3cPC/WtgYOo5DhhI_raw.srt
new file mode 100644
index 0000000000000000000000000000000000000000..788df139042be6aacafa373c8bcd642432f862bc
--- /dev/null
+++ b/PL9fwy3NUQKwZWgAb_V1ATlRRsH-6i3cPC/WtgYOo5DhhI_raw.srt
@@ -0,0 +1,2900 @@
+1
+00:00:02,060 --> 00:00:05,800
+السلام عليكم ورحمة الله وبركاته يعطيكم العافية
+
+2
+00:00:05,800 --> 00:00:09,280
+جميعًا اليوم المحاضرة بانوان Selective and
+
+3
+00:00:09,280 --> 00:00:13,560
+Differential Media في المحاضرة أو في المعامل
+
+4
+00:00:13,560 --> 00:00:17,540
+السابقة اتكلمنا عن الـ Media عرفنا الـ Media
+
+5
+00:00:17,540 --> 00:00:22,180
+وصنفنا الـ Media أو قسمنا الـ Media حسب الـ
+
+6
+00:00:22,180 --> 00:00:25,800
+Function حسب الـ Consistency حسب الـ Nutrient
+
+7
+00:00:25,800 --> 00:00:31,000
+وذكرنا عدة تصطيفات تحت كل صنف من هذه الأصناف
+
+8
+00:00:31,570 --> 00:00:36,270
+بالنسبة للـ function قلنا ممكن انها تكون general
+
+9
+00:00:36,270 --> 00:00:40,210
+media ممكن انها تكون transport media ممكن انها
+
+10
+00:00:40,210 --> 00:00:43,030
+تكون selective media ممكن انها تكون differential
+
+11
+00:00:43,030 --> 00:00:48,090
+media اليوم ماعملنا هنحكي فيه عن ال selective و ال
+
+12
+00:00:48,090 --> 00:00:52,610
+differential media هنشرح شو يعني selective شو يعني
+
+13
+00:00:52,610 --> 00:00:55,610
+differential و أمثلة على media selective و
+
+14
+00:00:55,610 --> 00:01:00,390
+differential media طيب بالأول خلينا نتكلم شو يعني
+
+15
+00:01:00,390 --> 00:01:04,740
+selectiveSelective بالعربي معناها انتقائي او
+
+16
+00:01:04,740 --> 00:01:10,780
+اختياري بدنا نختار و نمي نوع معين من الميكروبات
+
+17
+00:01:10,780 --> 00:01:14,800
+يبقى هي selection for the growth of certain
+
+18
+00:01:14,800 --> 00:01:19,160
+microorganism بدنا نمي نوع معين من الميكروبات
+
+19
+00:01:19,160 --> 00:01:25,360
+differential يعني تميزي بدي اميز كل نوع من هاي
+
+20
+00:01:25,360 --> 00:01:30,340
+الميكروبات اللي نمت بلون معينيبقى differential to
+
+21
+00:01:30,340 --> 00:01:34,720
+distinguish every group or species يبقى ال
+
+22
+00:01:34,720 --> 00:01:38,620
+selective راح ينمي ال group من ال micro organism
+
+23
+00:01:38,620 --> 00:01:43,660
+راح ييجي ال differential يعطي كل species في هذا ال
+
+24
+00:01:43,660 --> 00:01:49,000
+group إشي مميز عن التاني هذا بالنسبة لمعنى
+
+25
+00:01:49,000 --> 00:01:53,080
+selective و differential mediaممكن الميديا تكون
+
+26
+00:01:53,080 --> 00:01:57,920
+selective لحال ممكن تكون الميديا differential لحال
+
+27
+00:01:57,920 --> 00:02:02,100
+ممكن تكون الميديا selective و differential بنفس
+
+28
+00:02:02,100 --> 00:02:05,680
+الوقت يعني انها تنمي جروب من ال micro organism
+
+29
+00:02:05,680 --> 00:02:11,180
+بعدين الميديا نفسها راح اتميز تعمل differentiate
+
+30
+00:02:11,180 --> 00:02:14,700
+تعطي كل ال species في هذا الجروب اشي مميز عن
+
+31
+00:02:14,700 --> 00:02:19,080
+التاني طيب
+
+32
+00:02:19,440 --> 00:02:22,320
+in addition to general herbivores media which
+
+33
+00:02:22,320 --> 00:02:25,040
+allow the growth of most type of bacteria
+
+34
+00:02:25,040 --> 00:02:29,120
+microbiologists use specialized media to identify
+
+35
+00:02:29,120 --> 00:02:34,660
+or isolate specific group of bacteria شو الهدف من
+
+36
+00:02:34,660 --> 00:02:38,920
+ال media الهدف من ال media أني أنمي معظم
+
+37
+00:02:38,920 --> 00:02:42,320
+الميكروبات allow the growth of most type of
+
+38
+00:02:42,320 --> 00:02:47,660
+bacteria بدي أنا أنمي معظم الميكروبات لكن بقوللي
+
+39
+00:02:47,660 --> 00:02:54,770
+أنهالـ Microbiologist بيختار ال media أو بيختار
+
+40
+00:02:54,770 --> 00:02:59,750
+media خاصة عشان يعزل أنواع معينة من الميكروبات
+
+41
+00:02:59,750 --> 00:03:06,110
+ويعمل تعريف لإلها احنا قلنا او مرمعنا قبل كده ان
+
+42
+00:03:06,110 --> 00:03:10,490
+ال microbiologist بيختار ال media حسب نوع العينة
+
+43
+00:03:10,490 --> 00:03:14,630
+بكون انا عارفة ان والله مثلا عينة ال urine ال most
+
+44
+00:03:14,630 --> 00:03:19,920
+commonلأ ال pathogen in urine X وY وZ فبالتالي أنا
+
+45
+00:03:19,920 --> 00:03:25,440
+بختار media اتنمي هدول البكتيريا ال most common
+
+46
+00:03:25,440 --> 00:03:30,520
+pathogen in urine عشان اعزل هذه الأنواع المعينة من
+
+47
+00:03:30,520 --> 00:03:34,220
+الميكروبات وأعمل لها تعريف selective and
+
+48
+00:03:34,220 --> 00:03:38,620
+differential media are used to isolate or identify
+
+49
+00:03:38,620 --> 00:03:42,400
+particular organism أكيد ال selective و ال
+
+50
+00:03:42,400 --> 00:03:46,390
+differential mediaتستخدم لعزل نوع معين من
+
+51
+00:03:46,390 --> 00:03:51,430
+الميكروبات وتعريفها selective media هنمعرف شو هي
+
+52
+00:03:51,430 --> 00:03:55,030
+ال selective media وانا ذكرت التعريف في بداية
+
+53
+00:03:55,030 --> 00:04:00,070
+المعمل allow certain type of organism to grow بدها
+
+54
+00:04:00,070 --> 00:04:04,990
+تسمح لنوع معين فقط انه ينمو and inhibit the growth
+
+55
+00:04:04,990 --> 00:04:09,950
+of other organism وباقي ال organism هتعمله
+
+56
+00:04:09,950 --> 00:04:14,270
+inhibition مش هتعمله killهتعمله inhibition ل
+
+57
+00:04:14,270 --> 00:04:18,750
+growth of other organism طيب بقول ال selectivity
+
+58
+00:04:18,750 --> 00:04:24,090
+ال selective agent إيش هو؟ for example organism
+
+59
+00:04:24,090 --> 00:04:27,910
+that can utilize a given sugar are easily secreted
+
+60
+00:04:27,910 --> 00:04:31,050
+by making that sugar that only carbon source in
+
+61
+00:04:31,050 --> 00:04:35,750
+the mediaعلى سبيل المثال ذاكرها ال media مرت معنى
+
+62
+00:04:35,750 --> 00:04:39,770
+اللي هي ال Simon-Cytrate Agaric قولنا هذه ال media
+
+63
+00:04:39,770 --> 00:04:45,130
+تعتبر selective media ليش selective media لأن فقط
+
+64
+00:04:45,130 --> 00:04:48,790
+ال organism اللي عنده القدرة على استهلاك ال
+
+65
+00:04:48,790 --> 00:04:54,230
+citrate كمصدر لل carbon فقط هو اللي هينمو او فقط
+
+66
+00:04:54,230 --> 00:04:58,410
+هي البكتيريا اللي هتنمو لأن ال citrate هو المصدر
+
+67
+00:04:58,410 --> 00:05:03,950
+الوحيد كان لل carbon في هذه ال mediaعشان هيك يعتبر
+
+68
+00:05:03,950 --> 00:05:08,990
+ال sodium citrate في ميدية السمن سيطرات أجار هو ال
+
+69
+00:05:08,990 --> 00:05:14,550
+selective agent هو اللي خلّى انه فقط نوع معين
+
+70
+00:05:14,550 --> 00:05:17,290
+البكتيريا اللي عندها القدرة على استهلاك ال citrate
+
+71
+00:05:17,290 --> 00:05:21,130
+هي اللي تنمو أما ال other organism مش هيقدر ينمو
+
+72
+00:05:21,130 --> 00:05:24,870
+هيعمله inhibition أو مش هيقدر ينمو في الأصل كمان
+
+73
+00:05:24,870 --> 00:05:29,860
+تمام؟مثال على selective media أو وضّح فكرة شو يعني
+
+74
+00:05:29,860 --> 00:05:33,240
+selective media شو أنا بضيف في هذه ال media عشان
+
+75
+00:05:33,240 --> 00:05:40,300
+تصير selective media on
+
+76
+00:05:40,300 --> 00:05:43,560
+the other hand selective inhibition of some type
+
+77
+00:05:43,560 --> 00:05:47,900
+of microorganism can be achieved by adding dyes
+
+78
+00:05:47,900 --> 00:05:52,440
+antibiotic salt or specific inhibitor which affect
+
+79
+00:05:52,440 --> 00:05:55,740
+the metabolism or enzyme system of the organism
+
+80
+00:05:56,450 --> 00:06:06,550
+بقول لي ال selective agent ممكن يكون salt ممكن
+
+81
+00:06:06,550 --> 00:06:11,150
+يكون dyes ممكن يكون سبغات ال selective agent ممكن
+
+82
+00:06:11,150 --> 00:06:19,390
+يكون dyes ممكن يكون antibiotic ممكن يكون salt all
+
+83
+00:06:19,390 --> 00:06:22,530
+specific inhibitor بتأثر على ال metabolism و ال
+
+84
+00:06:22,530 --> 00:06:28,060
+enzyme ل الأورجانيزمهدول هم اللي هيخلوا او هيعملوا
+
+85
+00:06:28,060 --> 00:06:31,700
+inhibition ل ال other organism الستان و ال
+
+86
+00:06:31,700 --> 00:06:36,680
+antibiotic و السون فبالتالي ال other organism هي
+
+87
+00:06:36,680 --> 00:06:41,440
+اللي هتنمو فقط يبقى هذا يعتبر selective inhibition
+
+88
+00:06:41,440 --> 00:06:47,320
+بيعمل inhibition ل organism معين فبالتالي مش هيقدر
+
+89
+00:06:47,320 --> 00:06:52,260
+ينمو فقط ال other organism هو اللي هينمو ذا كل
+
+90
+00:06:52,260 --> 00:06:57,670
+أمثلةعلى سبيل المثال، مجموعة تحتوي على بوتاسيوم
+
+91
+00:06:57,670 --> 00:07:04,010
+توليرايت أو سوديوم أزايت أو ثاليوم أسيتات هدول
+
+92
+00:07:04,010 --> 00:07:09,950
+طبعا بتركيز واحد من عشرة لخمسة من عشرة سينهيبط
+
+93
+00:07:09,950 --> 00:07:15,490
+أكتر من جرام بكتيرية ناجحةتمام هدول هم ال
+
+94
+00:07:15,490 --> 00:07:19,290
+potassium tolerate و Sodium Azide و Phthalium
+
+95
+00:07:19,290 --> 00:07:24,270
+Acetate هدول كلهم يعتبروا ك selective agent
+
+96
+00:07:24,270 --> 00:07:30,010
+بيعملوا inhibition لجرام negative bacteria يبقى
+
+97
+00:07:30,010 --> 00:07:34,050
+هيكونوا لو أنا media اضفت عليها هدول التلاتة ال
+
+98
+00:07:34,050 --> 00:07:38,500
+salt طبعا هدول يعتبروا saltمن الأمثلة على الـ
+
+99
+00:07:38,500 --> 00:07:41,400
+Selective Inhibition بيعملوا Inhibition لـ Growth
+
+100
+00:07:41,400 --> 00:07:44,940
+of Gram Negative Bacteria يبقى هيكونوا Selection
+
+101
+00:07:44,940 --> 00:07:49,240
+لمين للجرام Positive Bacteria هيكونوا Selective
+
+102
+00:08:19,630 --> 00:08:26,070
+هدول طبعا ال salt أي
+
+103
+00:08:26,070 --> 00:08:30,630
+ميديا بتحتوي على هدول ال salt هاي الأملح بتعمل هاي
+
+104
+00:08:30,630 --> 00:08:33,970
+الأملح inhibition لجرام negative بكتيريا بالتالي
+
+105
+00:08:33,970 --> 00:08:37,770
+هتسمح بنمول جرام positive بكتيريا selection of
+
+106
+00:08:37,770 --> 00:08:43,230
+جرام positive بكتيرياطيب media supplement with
+
+107
+00:08:43,230 --> 00:08:46,970
+penicillin ال penicillin يعتبر antibiotic هاي مثال
+
+108
+00:08:46,970 --> 00:08:49,510
+احنا قولنا ممكن يكون dyes ممكن يكون antibiotic
+
+109
+00:08:49,510 --> 00:08:53,160
+ممكن يكون saltأمثلة على الـ Salt هيها التلاتة هذول
+
+110
+00:08:53,160 --> 00:08:56,960
+يعتبروا Salt قولنا بتعمل Inhibition لـ Growth of
+
+111
+00:08:56,960 --> 00:09:00,100
+Gram Negative Bacteria طيب الميديا اللي فيها
+
+112
+00:09:00,100 --> 00:09:03,520
+Penicillin ال Penicillin يعتبر Antibiotic هي مثال
+
+113
+00:09:03,520 --> 00:09:07,900
+على ال Antibiotic او ال crystal violet ال crystal
+
+114
+00:09:07,900 --> 00:09:13,240
+violet يعتبر Dyes استعينى يبقى هيك ذكرنا كل ذكرنا
+
+115
+00:09:13,240 --> 00:09:16,920
+مثال على كل نوع Dyes Antibiotic او Salt قولي ال
+
+116
+00:09:16,920 --> 00:09:20,580
+Penicillin و ال crystal violetبيعملوا inhibition
+
+117
+00:09:20,580 --> 00:09:25,380
+للجرام positive بكتيريا يبقى هي هتكون hand
+
+118
+00:09:25,380 --> 00:09:28,220
+selective لمين للجرام negative بكتيريا
+
+119
+00:09:47,230 --> 00:09:51,410
+يبقى أي ميديا فيها بنيسيلان وcrystal violet هتنمي
+
+120
+00:09:51,410 --> 00:09:56,050
+فقط لجرام نيجاتيف بكتيريا selective of جرام
+
+121
+00:09:56,050 --> 00:09:59,610
+نيجاتيف بكتيريا هتعمل inhibition للجرام positive
+
+122
+00:09:59,610 --> 00:10:06,510
+بكتيريا والبنيسيلان احنا قلنا في بداية المعاملإنه
+
+123
+00:10:06,510 --> 00:10:11,330
+الاستهداف لإيلولا الروابط الـ cross-link ما بين
+
+124
+00:10:11,330 --> 00:10:14,510
+الـ بيبتيدوش لايكان في الـ gram positive بكتيريا
+
+125
+00:10:14,510 --> 00:10:17,110
+فبالتالي احنا قلنا إنه هيأثر على الـ gram positive
+
+126
+00:10:17,110 --> 00:10:20,390
+بكتيريا ومش هيأثر على الـ gram negative بكتيريا
+
+127
+00:10:20,390 --> 00:10:26,740
+هذا كان ال action تبعه للبنسلان طيبهنذاكر إنه
+
+128
+00:10:26,740 --> 00:10:30,040
+ميديات نفس الكلام اللي فوق توليرايت أجار إيش يعني
+
+129
+00:10:30,040 --> 00:10:33,940
+توليرايت أجار يعني ميديا فيها بوتاسيوم توليرايت
+
+130
+00:10:33,940 --> 00:10:37,240
+قلنا هي ميديا بتعمل inhibition لليجرام negative
+
+131
+00:10:37,240 --> 00:10:41,480
+بكتيريا يبقى هي selective لليجرام positive بكتيريا
+
+132
+00:10:41,480 --> 00:10:45,320
+is used to select polygram positive organism هي
+
+133
+00:10:45,320 --> 00:10:50,260
+كاتب هي ميديا بتعمل هي بتختار بتعمل اختيار لليجرام
+
+134
+00:10:50,260 --> 00:10:53,500
+positive بكتيريا اتفقنا فوق هذا الكلام على هذا
+
+135
+00:10:53,500 --> 00:10:57,370
+الكلامبما انها بتعمل inhibition لجرام negative
+
+136
+00:10:57,370 --> 00:11:01,930
+بكتيريا يبقى هي select for gram positive بكتيريا
+
+137
+00:11:01,930 --> 00:11:05,630
+and nutrient agar supplement with penicillin لو
+
+138
+00:11:05,630 --> 00:11:10,290
+nutrient agar ضفت عليها penicillin احنا قلنا ال
+
+139
+00:11:10,290 --> 00:11:13,370
+penicillin بيعمل inhibition لجرام positive بكتيريا
+
+140
+00:11:13,370 --> 00:11:18,050
+يبقى هو select for gram negative organism هيك
+
+141
+00:11:18,050 --> 00:11:25,190
+بيكون المعلومات واضحةيعني الكلام هذا تقريبًا ذاكر
+
+142
+00:11:25,190 --> 00:11:27,930
+«هان» إنها بتعمل «inhibition» و «هان» ذاكر إنها
+
+143
+00:11:27,930 --> 00:11:32,430
+بتكون «selective» لمين يبقى أنا بحفظ ال «salt» هذا
+
+144
+00:11:32,430 --> 00:11:37,590
+بيعمل «inhibition» لمين يبقى بشكل بديهي بكون عارفة
+
+145
+00:11:37,590 --> 00:11:48,850
+«selective» لأي نوع من البكتيريا طيب
+
+146
+00:11:49,150 --> 00:11:52,290
+ذاكرنا شو يعني selective media نيجي لـ
+
+147
+00:11:52,290 --> 00:11:56,110
+differential media differential media doesn't
+
+148
+00:11:56,110 --> 00:12:00,250
+necessarily inhibit bacterial growth but instead
+
+149
+00:12:00,250 --> 00:12:03,430
+makes the bacteria look different بقول لي هي مش
+
+150
+00:12:03,430 --> 00:12:07,870
+بالضرورة انها تعمل inhibition لل growth للبكتيريا
+
+151
+00:12:07,870 --> 00:12:13,830
+لكن بتعمل تمييز لكل كولوني لكل species او كل group
+
+152
+00:12:13,830 --> 00:12:18,130
+بتخليها او بتعطيها شكل مميز كل group او كل species
+
+153
+00:12:18,600 --> 00:12:22,440
+أو every colony حتى differential media work best
+
+154
+00:12:22,440 --> 00:12:26,640
+with closely related organism and the differential
+
+155
+00:12:26,640 --> 00:12:32,100
+agent is what causes the bacteria look different
+
+156
+00:12:32,100 --> 00:12:35,200
+بقولي ال differential agent زي ما قولنا فيه
+
+157
+00:12:35,200 --> 00:12:37,880
+selective agent زي ال salt زي ال dye زي ال
+
+158
+00:12:37,880 --> 00:12:42,420
+antibiotic برضه في عندي differential agent مادة هي
+
+159
+00:12:42,420 --> 00:12:47,350
+اللي بتخلي البكتيريا مختلفةowing the presence of
+
+160
+00:12:47,350 --> 00:12:50,190
+certain dyes or chemical in the media the organism
+
+161
+00:12:50,190 --> 00:12:53,670
+will produce characteristic exchange or a growth
+
+162
+00:12:53,670 --> 00:12:56,610
+pattern that are used for identification or
+
+163
+00:12:56,610 --> 00:13:00,410
+differentiation بقول لي كمان ان هذا ال
+
+164
+00:13:00,410 --> 00:13:04,690
+differential agent ممكن يكون وجود هو عبارة عن وجود
+
+165
+00:13:04,690 --> 00:13:08,050
+presence of certain dyes or chemical موجودة في هذه
+
+166
+00:13:08,050 --> 00:13:11,550
+ال media ممكن يكون dyes ممكن يكون chemical موجود
+
+167
+00:13:11,550 --> 00:13:16,560
+في هذه ال media بيخلي ال organismيعمل نمو أو تغير
+
+168
+00:13:16,560 --> 00:13:21,420
+في النمو أو في نمط النمو وهذا هيساعد في ال
+
+169
+00:13:21,420 --> 00:13:25,840
+identification و ال differentiation هنفهم أكتر لما
+
+170
+00:13:25,840 --> 00:13:28,900
+نذكر أمثلة على selective و differential media
+
+171
+00:13:28,900 --> 00:13:31,800
+نتعرف مين هو ال selective agent كيف هذا ال
+
+172
+00:13:31,800 --> 00:13:35,380
+selective agent بيميز كل كولوني أو كل species أو
+
+173
+00:13:35,380 --> 00:13:39,860
+group في هذه ال mediaA variety of selective and
+
+174
+00:13:39,860 --> 00:13:42,840
+differential media are used in medical diagnostic
+
+175
+00:13:43,450 --> 00:13:47,110
+and water pollution laboratories and in food and
+
+176
+00:13:47,110 --> 00:13:51,530
+dairy laboratories بقوللي الـ Selective و الـ
+
+177
+00:13:51,530 --> 00:13:56,830
+Differential media بتستخدم في التشخيص الطبي زي ما
+
+178
+00:13:56,830 --> 00:14:02,130
+احنا كخصائي تحاليل طبية نستخدم الـ Differential و
+
+179
+00:14:02,130 --> 00:14:05,290
+الـ Selective media عشان اعمل identification
+
+180
+00:14:05,290 --> 00:14:11,510
+لmicro organism موجود لعندي عشان اعرف شو المسبب
+
+181
+00:14:11,510 --> 00:14:18,240
+للالتهابكمان يستخدم في تلوث المياه بيفلتره طبعا
+
+182
+00:14:18,240 --> 00:14:22,640
+المياه عشان يشوفوا الكوليفارم بعدين بيحطوها على
+
+183
+00:14:22,640 --> 00:14:29,220
+طبق ال AMB طبعا طبق ال AMB هنتعرف عليها في هذا ال
+
+184
+00:14:29,220 --> 00:14:34,480
+chapter ال AMB بتعطي ال equalize لون أخضر ال green
+
+185
+00:14:34,480 --> 00:14:38,680
+metallic chain فبالتالي هيك بتمييزها و بيسهل علي
+
+186
+00:14:38,680 --> 00:14:43,710
+تعريفها و ال identification لإلهاكمان برضه في اللي
+
+187
+00:14:43,710 --> 00:14:48,350
+هو مختبرات الـ Food ومراقبة وجودة الأغذية تقريبا
+
+188
+00:14:48,350 --> 00:14:51,310
+يعني في كل المختبرات تستخدم الـ Selective و الـ
+
+189
+00:14:51,310 --> 00:14:53,970
+Differential Media عشان تسهل عليها ال
+
+190
+00:14:53,970 --> 00:14:59,230
+identification والتعريف Some
+
+191
+00:14:59,230 --> 00:15:02,010
+media are both selective and differential احنا
+
+192
+00:15:02,010 --> 00:15:04,890
+قلنا في بعض أنواع ال media ممكن انها تكون
+
+193
+00:15:04,890 --> 00:15:08,750
+selective و differential في نفس الوقتthat is that
+
+194
+00:15:08,750 --> 00:15:12,090
+are able to select to select against the growth of
+
+195
+00:15:12,090 --> 00:15:17,570
+certain organism بقوللي هي بتسمح انها لنمو نوع
+
+196
+00:15:17,570 --> 00:15:21,970
+معين من الميكروبات او بتنمي نوع معين من الميكروبات
+
+197
+00:15:21,970 --> 00:15:27,240
+while the organism that do grow my eggssome
+
+198
+00:15:27,240 --> 00:15:31,580
+differential growth characteristic بقوللي ان اللي
+
+199
+00:15:31,580 --> 00:15:36,540
+اللي نمت من الميكروبات في هذه ال media بتمييزها
+
+200
+00:15:36,540 --> 00:15:43,080
+وبتعطيها اشي مختلف او لون مختلف عن ال عن كل group
+
+201
+00:15:43,080 --> 00:15:48,140
+او species there of the more common selective and
+
+202
+00:15:48,140 --> 00:15:51,360
+differential media are described below and will be
+
+203
+00:15:51,360 --> 00:15:55,840
+used in the laboratory exercise هنا في هذا الشابط
+
+204
+00:15:55,840 --> 00:15:59,780
+اذاكرتلت أمثلة على selective و differential media
+
+205
+00:15:59,780 --> 00:16:02,900
+أول
+
+206
+00:16:02,900 --> 00:16:07,680
+media هنتعرف عليها اللي هي ال money tall, salt,
+
+207
+00:16:07,880 --> 00:16:15,980
+agar بس هان في خطأ كتابي او هو ليس I money tall,
+
+208
+00:16:16,320 --> 00:16:22,820
+salt, agar هذه ال media هي selection of استفيل ككة
+
+209
+00:16:23,570 --> 00:16:28,190
+وبتعمل inhibition لـother organism يعني بتعمل
+
+210
+00:16:28,190 --> 00:16:33,710
+inhibition لجرام negative بكتيريا لجرام positive
+
+211
+00:16:33,710 --> 00:16:39,430
+غير لستافيلو كوكاية تبقى فقط بتنمي لستافيلو كوكاية
+
+212
+00:16:39,430 --> 00:16:44,650
+طيب شو اللي خلى في هذه ال media فقط لستافيلو
+
+213
+00:16:44,650 --> 00:16:48,530
+كوكاية انها اللي تنمو بقول في عندي selective agent
+
+214
+00:16:48,530 --> 00:16:51,990
+احنا قولنا selective agent ممكن يكون solved في هذه
+
+215
+00:16:51,990 --> 00:16:56,780
+ال mediaهو Sod التركيز العالي من ال Sodium
+
+216
+00:16:56,780 --> 00:17:02,500
+Chloride 7.5% NaCl هيعمل Inhibition لكل ال
+
+217
+00:17:02,500 --> 00:17:06,360
+organism مع دا الاستفيلوكوكاي لأن الاستفيلوكوكاي
+
+218
+00:17:06,360 --> 00:17:11,220
+من خصائصها انها بتتحمل درجة الملوحة العالية احنا
+
+219
+00:17:11,220 --> 00:17:16,800
+قلنا تركيز ال NaCl 85% من 100% تخيل انه يكون
+
+220
+00:17:16,800 --> 00:17:21,820
+التركيز 7.5% يبقى كل ال micro organism هيعملها
+
+221
+00:17:21,820 --> 00:17:25,370
+Inhibitionفقط لاستفيد الكوكايل عندها القدرة إنها
+
+222
+00:17:25,370 --> 00:17:29,450
+تتحمل درجة الملوحة العالية هي اللي هتنقل يبقى هذا
+
+223
+00:17:29,450 --> 00:17:31,990
+الـ Selective agent اللي هو التركيز العالي من
+
+224
+00:17:31,990 --> 00:17:36,950
+الأملح بالنسبة لـ Differential agent هذه ال media
+
+225
+00:17:36,950 --> 00:17:41,830
+تعتبر Selective and Differential agent مين هو الـ
+
+226
+00:17:41,830 --> 00:17:45,230
+Differential agent الـ Differential agent في هذه
+
+227
+00:17:45,230 --> 00:17:50,390
+ال media المانيتول المانيتول يعتبر sugar طيب
+
+228
+00:17:50,600 --> 00:17:53,660
+البكتيريا اللى نمت لستافيلو كوكاي في هذه الميديا
+
+229
+00:17:53,660 --> 00:17:57,860
+بقول لي لستافيلو كوكاي بدي اميزها بدي اميز ال
+
+230
+00:17:57,860 --> 00:18:01,600
+species للاستافيلو كوكاى Differentiate استافيلو
+
+231
+00:18:01,600 --> 00:18:05,260
+كوكاى ال species يبقى ال differential agent هو
+
+232
+00:18:05,260 --> 00:18:10,700
+المانيدال الشجر عشان نعرف كيف تعمل differentiate
+
+233
+00:18:10,700 --> 00:18:15,340
+بين الاستافيلو كوكاى ال species بنا نعرف ان في هذه
+
+234
+00:18:15,340 --> 00:18:20,770
+الميديا BH Indicator اللى هو الفينال Redالفيه non
+
+235
+00:18:20,770 --> 00:18:24,250
+-red مر معانا للمرة الرابعة اول مرة في اليوريا
+
+236
+00:18:24,250 --> 00:18:27,270
+المرة التانية فيه او اول مرة في ال fermentation
+
+237
+00:18:27,270 --> 00:18:30,630
+تانية مرة في اليوريا تالت مرة في ال triple sugar
+
+238
+00:18:30,630 --> 00:18:34,830
+iron agar وهي في ال manitol salt agar حدوده في ال
+
+239
+00:18:34,830 --> 00:18:38,130
+acid yellow في ال base بيكون non-red بقولي
+
+240
+00:18:38,130 --> 00:18:42,510
+البكتيريا اللى عندها الاستفيله كوكاي طبعا هي فقط
+
+241
+00:18:42,510 --> 00:18:45,670
+اللى هتتحمل الملوحة العالية فقط اللى هتنمو على ال
+
+242
+00:18:45,670 --> 00:18:50,110
+manitol salt agarبننميز الستافيلو كوكايا ال
+
+243
+00:18:50,110 --> 00:18:53,230
+species بقول ليه فى من الستافيلو كوكايا عندها
+
+244
+00:18:53,230 --> 00:18:57,210
+القدرة انها تعمل fermentation للمانيتال وفى non
+
+245
+00:18:57,210 --> 00:19:00,550
+manitol fermenter طيب البكتيريا اللى عندها القدرة
+
+246
+00:19:00,550 --> 00:19:05,350
+انها تعمل fermentation للمانيتال هتعمل ferment
+
+247
+00:19:05,350 --> 00:19:08,270
+للمانيتال طبعا المانيتال شجر هيصله fermentation
+
+248
+00:19:09,320 --> 00:19:12,860
+هتعمله fermentation هتعمل fermentation للمانيتال
+
+249
+00:19:12,860 --> 00:19:17,500
+نواتج عملية ال fermentation acid هتعمل على خفض ال
+
+250
+00:19:17,500 --> 00:19:23,680
+pH هيتحول لون الوسط للون yellow يبقى colonies
+
+251
+00:19:23,680 --> 00:19:28,740
+surrounded by yellow zone هي بتكون معناها انه
+
+252
+00:19:28,740 --> 00:19:32,160
+manitol fermenter من الأمثلة على ال manitol
+
+253
+00:19:32,160 --> 00:19:36,510
+fermenter اللي استفيله gas aureusطب لو البكتيريا
+
+254
+00:19:36,510 --> 00:19:39,730
+الاستفيله ككاى ال species ماعندها القدرة انها تعمل
+
+255
+00:19:39,730 --> 00:19:45,610
+fermentation للمانيتال مش هيتم انتج اي تغير في
+
+256
+00:19:45,610 --> 00:19:50,690
+اللون فبالتالي هيظلوا ال colony لونها colorless و
+
+257
+00:19:50,690 --> 00:19:54,510
+لون الطبق لونه pink من الأمثلة عليها الاستفيله
+
+258
+00:19:54,510 --> 00:19:58,950
+ككاس ابدامتر يبقى لو مسكنا طبق manitol salt agar
+
+259
+00:19:58,950 --> 00:20:03,510
+لقنا بكتيريا نامية يبقى بعرف انه هيستفيله ككاىلأن
+
+260
+00:20:03,510 --> 00:20:08,270
+هذه الميديا مختارة من استفيله و ككاية فقط اللي
+
+261
+00:20:08,270 --> 00:20:13,010
+هتنمو لستفيله و ككاية طيب أعطتني لون أصفر يبقى
+
+262
+00:20:13,010 --> 00:20:17,750
+بعرف أنه عملت Manitol Fermenter شو يعني Manitol
+
+263
+00:20:17,750 --> 00:20:20,690
+Fermenter يعني عملت fermentation للمانيتل اتكون
+
+264
+00:20:20,690 --> 00:20:25,510
+أسد عمل على حفظ ال BH فبالتالي اتحول لون الوسط
+
+265
+00:20:25,510 --> 00:20:29,730
+للون أصفريبقى لون الوسط لون الـmedia لونها زهري
+
+266
+00:20:29,730 --> 00:20:34,210
+هلاقي ان الـmedia تحول لونها لللون الأصفر وممكن
+
+267
+00:20:34,210 --> 00:20:37,170
+إذا اتكون كمية كبيرة من الأسد ألاقي الـcolony
+
+268
+00:20:37,170 --> 00:20:40,650
+نفسها تحولت للون الأصفر من الأمثلة عليها
+
+269
+00:20:40,650 --> 00:20:44,830
+الاستافيلوكوكا او الاسم طيب لو مسكت الطبق ولاقيت
+
+270
+00:20:44,830 --> 00:20:48,390
+والله عندي كولوني لونها colorless لكن الطبق
+
+271
+00:20:48,390 --> 00:20:51,590
+حوالينه ضاله لونه pink يبقى بعرف ان البكتيريا
+
+272
+00:20:51,590 --> 00:20:54,630
+استافيلوكوكاي لأن الـmedia selection of
+
+273
+00:20:54,630 --> 00:20:59,080
+استافيلوكوكاطيب لونها colorless و لون ال media
+
+274
+00:20:59,080 --> 00:21:02,900
+دلوا لونه pink يبقى بعرف انه هي non manitol
+
+275
+00:21:02,900 --> 00:21:07,400
+fermenter ماعملت fermentation للمنيتول ماتغير لون
+
+276
+00:21:07,400 --> 00:21:12,500
+ال media دلوا لون ال media pink و دل لون ال كله
+
+277
+00:21:12,500 --> 00:21:16,580
+colorless من الأمثلة عليها لسه فيه كوكس epidermis
+
+278
+00:21:16,580 --> 00:21:22,920
+هذا بالنسبة لمنيتول salt agaricManitol salt agar
+
+279
+00:21:22,920 --> 00:21:25,580
+is a selective media هي selective و differential
+
+280
+00:21:25,580 --> 00:21:29,960
+media لكن هو هان بده يشرحش يعني selective media
+
+281
+00:21:29,960 --> 00:21:33,540
+used for isolation of pathogenic Staphylococci
+
+282
+00:21:33,540 --> 00:21:37,680
+تستخدم لعزل الـ Staphylococci لأن هي فقط بتنمي الـ
+
+283
+00:21:37,680 --> 00:21:41,080
+Staphylococci الميديا بتحتوي على Manitol ك
+
+284
+00:21:41,080 --> 00:21:44,380
+differential agent في non-red indicator كبيئة
+
+285
+00:21:44,380 --> 00:21:49,560
+Indicator 7.5% Sodium chloride ك selective agent
+
+286
+00:21:49,970 --> 00:21:53,470
+Ferment Manitol هو اللي هيعمل لديه Differentiate
+
+287
+00:21:53,470 --> 00:21:55,830
+هو الـDifferentiation اللي فيه Nordred هو الـBH
+
+288
+00:21:55,830 --> 00:21:58,770
+Indicator قولنا الـSelective الـHigh Salt
+
+289
+00:21:58,770 --> 00:22:01,970
+Concentration التركيز العالي من الأملح هيعمل
+
+290
+00:22:01,970 --> 00:22:05,510
+Inhibition لمعظم of most بكتيريا other than
+
+291
+00:22:05,510 --> 00:22:09,550
+Staphylococci معادل Staphylococci بقولي التركيز
+
+292
+00:22:09,550 --> 00:22:12,450
+العالي من الأملح مش هيعمل كله لـGram Negative
+
+293
+00:22:12,450 --> 00:22:16,470
+بكتيريا هيعمل Inhibition اتفقنا انه ما بيصير كله
+
+294
+00:22:16,470 --> 00:22:22,900
+بيصير Inhibition لهلـ Growth لـ Other Organism On
+
+295
+00:22:22,900 --> 00:22:27,280
+Manitouin Salt Agar باثوجينك إستفيلوكوكس أوريس
+
+296
+00:22:27,280 --> 00:22:30,740
+طبعا الباثوجينك من الإستفيلوكوكس هي الإستفيلوكوكس
+
+297
+00:22:30,740 --> 00:22:34,480
+أوريس produce small colonies surrounded by yellow
+
+298
+00:22:34,480 --> 00:22:39,400
+zone اتفقنا بتفرج طبعا هي بتكون small colony محيطة
+
+299
+00:22:39,400 --> 00:22:43,680
+باللون الأصفر نتيجة fermentation لـ Manitouinالسبب
+
+300
+00:22:43,680 --> 00:22:46,800
+في تغيير اللون دا تستفيل الكوكس Aureus هي ferment
+
+301
+00:22:46,800 --> 00:22:50,540
+the manitol producing an acid which is turned
+
+302
+00:22:50,540 --> 00:22:53,260
+trans the indicator from red to yellow هذا هو
+
+303
+00:22:53,260 --> 00:22:58,160
+السبب اللي بيخلي تستفيل الكوكس Aureus ان الكلوني
+
+304
+00:22:58,160 --> 00:23:01,320
+حولها لون أصفر نتيجة ال fermentation للمانيتل
+
+305
+00:23:01,320 --> 00:23:03,880
+تتكون acid عمل على خفض البيئة مش اتحول ال
+
+306
+00:23:03,880 --> 00:23:08,060
+indicator من red ليرد لكن الأخر لستفيل الكوكس ما
+
+307
+00:23:08,060 --> 00:23:12,650
+بتعمل fermentation للمانيتلمش هيتم إنتاج أي تغير
+
+308
+00:23:12,650 --> 00:23:17,490
+في لون الميديا اللي هو أصلا بنكلر تمام بيقول ال
+
+309
+00:23:17,490 --> 00:23:20,230
+growth of other types of bacteria is generally
+
+310
+00:23:20,230 --> 00:23:24,450
+inhibited ال organism الآخر غير الاستافيولوكوكاي
+
+311
+00:23:24,450 --> 00:23:29,430
+بيصير لها inhibition وليس killed طيب نيجي لكل نوع
+
+312
+00:23:29,430 --> 00:23:32,090
+من أنواع البكتيريا الاستافيولوكوكاس اورياس على
+
+313
+00:23:32,090 --> 00:23:37,080
+المانيتواس هولد أجار أكيدهى Salt Tolerant هتتحمل
+
+314
+00:23:37,080 --> 00:23:39,720
+الملوحة العالية فبالتالي هتنمو اى بكتيرى
+
+315
+00:23:39,720 --> 00:23:45,620
+Staphylococci طبعا هتنمو لانها هتتحمل درجة الملوحة
+
+316
+00:23:45,620 --> 00:23:51,420
+العالية تمام بالنسبة لStaphylococcus aureus ايش
+
+317
+00:23:51,420 --> 00:23:54,840
+هتعمل هتعمل fermentation للمنيطول فهيتحول لون ال
+
+318
+00:23:54,840 --> 00:23:58,160
+media للون يلو Staphylococcus epidermidis برضه
+
+319
+00:23:58,160 --> 00:24:01,400
+هتتحمل الملوحة العالية بما انها Staphylococciلكن
+
+320
+00:24:01,400 --> 00:24:04,620
+مش هتعمل fermentation للمانيتال فهيضلوا لون ال
+
+321
+00:24:04,620 --> 00:24:08,900
+media لون big ال micrococcus luteus not salt
+
+322
+00:24:08,900 --> 00:24:12,680
+tolerant doesn't growth of own manitol نمسح هذه
+
+323
+00:24:12,680 --> 00:24:17,680
+الجملة ال
+
+324
+00:24:17,680 --> 00:24:21,600
+micrococcus بقول لي نفس الاستفيله coccus ابتنموا
+
+325
+00:24:21,600 --> 00:24:25,540
+على ال manitol salt agar و بتعمل fermentation
+
+326
+00:24:25,540 --> 00:24:29,760
+للمانيتال طيب احنا قلنا انه فقط ال manitol salt
+
+327
+00:24:29,760 --> 00:24:34,230
+agarهي اللي بتنمو على ميدية الـ Manitoulin salt
+
+328
+00:24:34,230 --> 00:24:38,490
+agar كيف بنحكي أنه ال micro ككس نفس لسه فيه ككس
+
+329
+00:24:38,490 --> 00:24:43,670
+aureus بتنمو و بتعطي اللون الأصفر بدنا نعرف معلومة
+
+330
+00:24:43,670 --> 00:24:47,530
+never ever trust is in selectivity of the
+
+331
+00:24:47,530 --> 00:24:51,070
+selective media مابدي أثق في ال selective media
+
+332
+00:24:51,070 --> 00:24:54,370
+لازم انا اعمل فحوصات اعمل جرامستين اعمل فحوصات
+
+333
+00:24:54,370 --> 00:24:58,650
+كيميائية هي بتسهل علي التعريف لكن انامش من حد ما
+
+334
+00:24:58,650 --> 00:25:01,630
+افتح طبق الـ Manitoula Salt Agar أشوف اللون أصفر
+
+335
+00:25:01,630 --> 00:25:05,350
+خلص على طول الـ Staphylococcus Aureus لأ لازم أعمل
+
+336
+00:25:05,350 --> 00:25:09,990
+إجرامستان لازم أمشي فحوصات لازم أمشي كل الفحوصات
+
+337
+00:25:09,990 --> 00:25:13,690
+حتى أوصل لتعريفي نهائي فقط بعرف أنه هي عملت
+
+338
+00:25:13,690 --> 00:25:17,410
+fermentation لالـ Manitoula من ميدية الاستفيلو من
+
+339
+00:25:17,410 --> 00:25:21,250
+ميدية الـ Manitoula Salt Agar يبقى هنا فيه طبعا
+
+340
+00:25:21,250 --> 00:25:24,850
+دراسات حديثة بتأكد أنه ال micrococcus نفس لـ
+
+341
+00:25:24,850 --> 00:25:28,320
+Staphylococcus Aureus علىالـ Manifold Salt Agar
+
+342
+00:25:28,320 --> 00:25:33,080
+بتنمو وبتعطي اللون الأصفر فبالتالي بدنا نستفيد من
+
+343
+00:25:33,080 --> 00:25:36,100
+هذه المعلومة إنه «never ever trust in selectivity
+
+344
+00:25:36,100 --> 00:25:41,440
+of selective media»
+
+345
+00:25:41,440 --> 00:25:46,960
+طيب بالنسبة للصور هذه الصور ممكن إنه يجينا في
+
+346
+00:25:46,960 --> 00:25:49,820
+الامتحان هذا طبق Manifold Salt Agar ال media لون
+
+347
+00:25:49,820 --> 00:25:52,760
+ال media هيه لون زهري نفس اللون الموجود هنا اللي
+
+348
+00:25:52,760 --> 00:25:58,370
+أنا بأشهر عليهزراعة بكتيريتان بكتيريا أعطتني حولت
+
+349
+00:25:58,370 --> 00:26:02,610
+اللون حولان الكلوني لون أصفر وحتى لون الكلوني لون
+
+350
+00:26:02,610 --> 00:26:07,910
+أصفر لكن هنا بلاحظ أنه أظل اللون pink يبقى high
+
+351
+00:26:07,910 --> 00:26:12,270
+بما أنها نمط بحكي أنه هي استفيله ككاية طيب أو نحكي
+
+352
+00:26:12,270 --> 00:26:16,950
+سؤال tolerant اتحملت الملوحة العالية طيب نيجي ل
+
+353
+00:26:20,090 --> 00:26:23,110
+الـ fermentation بما انه اتحول اللون للون أصفر
+
+354
+00:26:23,110 --> 00:26:26,390
+يبقى بحكي Manitol Fermenter لكن هنا بحكي not
+
+355
+00:26:26,390 --> 00:26:31,010
+Manitol .. non Manitol Fermenter يبقى هذه احتمال
+
+356
+00:26:31,010 --> 00:26:34,950
+انها تكون مثال عليها لسه في الكوكس Aureus اللي
+
+357
+00:26:34,950 --> 00:26:38,770
+بتعطي هذا اللون وهي لسه في الكوكس Epidermis هنا
+
+358
+00:26:38,770 --> 00:26:41,470
+نفس الشيء هم اللاحظ اللون أصفر وهنا اللون أحمر
+
+359
+00:26:41,470 --> 00:26:44,910
+يبقى هاي Manitol Fermenter هاي Non Manitol
+
+360
+00:26:44,910 --> 00:26:50,160
+Fermenterهنا نلاحظ أن الطبق و الكلونيه صار لونها
+
+361
+00:26:50,160 --> 00:26:55,160
+أصفر يبقى هي بما أنها نمط يبقى هي salt tolerant من
+
+362
+00:26:55,160 --> 00:26:58,000
+الأمثلة عليها الاستفيل و الككاية طيب أعطت اللون
+
+363
+00:26:58,000 --> 00:27:01,800
+الأصفر يبقى manitol fermenter من الأمثلة عليها
+
+364
+00:27:01,800 --> 00:27:07,290
+الاستفيل و الككاسنمت يبقى البكتيريا salt tolerant
+
+365
+00:27:07,290 --> 00:27:12,790
+ما عضت اللون الأصفر، دا اللون ال media لونه pink و
+
+366
+00:27:12,790 --> 00:27:16,350
+ال colony كانت colorless، يبقى هي non-many-tol
+
+367
+00:27:16,350 --> 00:27:18,890
+fermenter من الأمثلة عليها الاستفيل و الككس
+
+368
+00:27:18,890 --> 00:27:22,890
+Epidermitis هاي ال media ماني تول sold أجار، لكن
+
+369
+00:27:22,890 --> 00:27:27,230
+ما لاحظت أي نمو، يبقى هي بالاصل ما اتحملت الملوحة
+
+370
+00:27:27,230 --> 00:27:30,590
+العالية، ماكان في عندي نمو، يبقى هاي احتمال أنها
+
+371
+00:27:30,590 --> 00:27:34,980
+تكون أي بكتيريا ما عدا الاستفيل و الككسبشكل عام
+
+372
+00:27:34,980 --> 00:27:37,640
+بنمحي الـ «micrococcus» قولنا لأنها بتنمو على
+
+373
+00:27:37,640 --> 00:27:41,960
+المانيتال سولت أجار ممكن نكتب اللي هي أي ميديا
+
+374
+00:27:41,960 --> 00:27:47,560
+إجرام positive كوكاي مثلاً الـ Clostridium البصلص
+
+375
+00:27:47,560 --> 00:27:52,380
+الستريبتو كوكاي أي بكتيريا إجرام positive أو إجرام
+
+376
+00:27:52,380 --> 00:27:56,380
+إجرام positive طبعاً من غير الاستافيرو كوكاي أو
+
+377
+00:27:56,380 --> 00:27:59,620
+إجرام negative بكتيريا هاي لو أنا نميتها على
+
+378
+00:27:59,620 --> 00:28:03,560
+المانيتال سولت أجار مش هتنمولأنها مش هتتحمل درجة
+
+379
+00:28:03,560 --> 00:28:07,380
+الملوحة العالية يبقى لو جيبنا في الامتحان سؤال انه
+
+380
+00:28:07,380 --> 00:28:12,000
+طبق منيطول salt agar لكن ما لاحظنا فيه نمو بنحكي
+
+381
+00:28:12,000 --> 00:28:17,660
+no growth because البكتيريا ماقدرة تتحمل non-salt
+
+382
+00:28:17,660 --> 00:28:21,020
+tolerant ماقدرة تتحمل الملوحة العالية يبقى هي أي
+
+383
+00:28:21,020 --> 00:28:24,660
+بكتيريا غير الاستافيلوكوكايو غير الميكروكوكاس
+
+384
+00:28:24,660 --> 00:28:28,580
+اذكري اي مثال مثلا gram negative bacteria
+
+385
+00:28:28,580 --> 00:28:34,080
+Sodamonas E.coli KlebsiellaStriptococci اي مثال من
+
+386
+00:28:34,080 --> 00:28:38,460
+ما عدا الاستفيلوكوكاي والمايكروكوكا هكذا نكون
+
+387
+00:28:38,460 --> 00:28:45,020
+خلصنا الأول ميديا اللي هي ال Manitol salt agar
+
+388
+00:28:45,020 --> 00:28:47,960
+selective و differential media تعرفنا شو ال
+
+389
+00:28:47,960 --> 00:28:51,700
+selective agent وشو ال differential agent طيب نيجي
+
+390
+00:28:51,700 --> 00:28:56,800
+لتاني ميديا using methylene blue agar هذه الميديا
+
+391
+00:28:56,800 --> 00:29:01,560
+برضه تعتبر selective و differential media تمامطيب
+
+392
+00:29:01,560 --> 00:29:04,140
+شو الـ selective agent في هذه الميديا الـ
+
+393
+00:29:04,140 --> 00:29:08,000
+selective agent هو اليوزين و الميثيليني بلو هدول
+
+394
+00:29:08,000 --> 00:29:11,560
+يعتبروا dyes قلنا ال selective agent ممكن يكون
+
+395
+00:29:11,560 --> 00:29:15,400
+dyes أمثلة على ميديا ال selective agent فيها dyes
+
+396
+00:29:15,400 --> 00:29:20,340
+هي اليوزين و الميثيليني بلو طيب هذه الميديا بقولي
+
+397
+00:29:20,340 --> 00:29:28,840
+بتعمل inhibition لجرام positive bacteria فبالتالي
+
+398
+00:29:28,840 --> 00:29:33,880
+هتكون selectionOr of Gram Negative Bacteria هتعمل
+
+399
+00:29:33,880 --> 00:29:38,220
+Inhibition لـ Gram Positive Bacteria فبالتالي
+
+400
+00:29:38,220 --> 00:29:41,680
+هتعمل Selection فقط اللي هتنمو هي لـ Gram Negative
+
+401
+00:29:41,680 --> 00:29:45,240
+Bacteria مين اللي عمل Inhibition لـ Gram Positive
+
+402
+00:29:45,240 --> 00:29:47,900
+Bacteria بقولي الـ Dice الايوزين والميثاليني ال
+
+403
+00:29:47,900 --> 00:29:50,840
+blue عملت Inhibition لـ Gram Positive Bacteria
+
+404
+00:29:50,840 --> 00:29:54,820
+ففقط اللي هتنمو هي لـ Gram Negative Bacteria هيك
+
+405
+00:29:54,820 --> 00:29:57,960
+أعرفنا مين اللي هينمو في هذه ال mediaالـ
+
+406
+00:29:57,960 --> 00:30:00,520
+Differential agent لـ Lactose كانت في الـ
+
+407
+00:30:00,520 --> 00:30:02,860
+Manitoulin Salt أجار الـ Manitoulin لكن هنا الـ
+
+408
+00:30:02,860 --> 00:30:06,800
+Lactose يبقى أي بكتيريا من الـ Gram Negative
+
+409
+00:30:06,800 --> 00:30:12,420
+بكتيريا نمت في هذا الطبق هيميز لي بين الـ Gram
+
+410
+00:30:12,420 --> 00:30:16,240
+Negative بكتيريا هل هي Lactose Fermenter أو Non
+
+411
+00:30:16,240 --> 00:30:20,220
+-Lactose Fermenter بشكل خاص هذه الميديا تستخدم للـ
+
+412
+00:30:20,220 --> 00:30:23,320
+Differentiate between coliform اللي هي تعتبر
+
+413
+00:30:23,320 --> 00:30:27,620
+Lactose Fermenternon-coliform bacteria فهي من عقلة
+
+414
+00:30:27,620 --> 00:30:30,640
+الـ Enterobacteriasi قلنا بتتقسم لـ coliform و non
+
+415
+00:30:30,640 --> 00:30:33,540
+-coliform أو lactose fermenter و non-lactose
+
+416
+00:30:33,540 --> 00:30:37,540
+fermenter ففي هذه الـ media أنا بفرق بين الـ
+
+417
+00:30:37,540 --> 00:30:39,920
+lactose fermenter من عقلة الـ Enterobacteriasi و
+
+418
+00:30:39,920 --> 00:30:42,740
+non-lactose fermenter من عقلة الـ Enterobacteriasi
+
+419
+00:30:42,740 --> 00:30:47,060
+لكن هذا لا يعني أن هذه البكتيريا فقط بتنمي الـ
+
+420
+00:30:47,060 --> 00:30:50,240
+Enterobacteriasi بتنمي كل gram negative بكتيريا
+
+421
+00:30:50,240 --> 00:30:56,610
+عشان هك لازم أعمل قبل ماأعمل مثلًا تعريف أو أحكي
+
+422
+00:30:56,610 --> 00:30:59,930
+إنه انتيرو بكترياسي لازم قبلها أعمل إيجرامستان
+
+423
+00:30:59,930 --> 00:31:03,050
+لازم قبلها أعمل فحص الـ Oxidase فحص الـGlucose
+
+424
+00:31:03,050 --> 00:31:06,370
+fermentation لأن في عندي صدوموناس في عندي Vibrio
+
+425
+00:31:06,370 --> 00:31:08,870
+cholera لازم أتأكد من هذه الأمور أعرف إن هي من
+
+426
+00:31:08,870 --> 00:31:12,110
+عائلة الانتيرو بكترياسي إذا أتأكدت إن هي من عائلة
+
+427
+00:31:12,110 --> 00:31:15,410
+الانتيرو بكترياسي بعرف بعدها هل هي lactose
+
+428
+00:31:15,410 --> 00:31:18,190
+fermenter أو non-lactosefermenter من خلال هذه
+
+429
+00:31:18,190 --> 00:31:22,130
+الـmediumالـ pH Indicator في هذه الميديا بقول لي
+
+430
+00:31:22,130 --> 00:31:26,710
+إن الصبغات بتشتغل as pH Indicator الـ Eucalyptus
+
+431
+00:31:26,710 --> 00:31:32,050
+Blue بتشتغل as pH Indicator طيب قلنا إن الـ Gram
+
+432
+00:31:32,050 --> 00:31:35,130
+Negative Bacteria هي اللي فقط بتنمو على الـ AMP
+
+433
+00:31:35,130 --> 00:31:38,230
+نمت بكتيريا يبقى و أعرف إن هي Gram Negative
+
+434
+00:31:38,230 --> 00:31:42,710
+Bacteria طيب بدي أميز هل هي lactose fermenter أو
+
+435
+00:31:42,710 --> 00:31:44,870
+non-lactose fermenter لأنه احنا قلنا الـ
+
+436
+00:31:44,870 --> 00:31:49,830
+differential agent هو اللاكتوز بقول لي لوالبكتيريا
+
+437
+00:31:49,830 --> 00:31:56,090
+طبعا كانت lactose fermenter هتنتج acid تمام هذا ال
+
+438
+00:31:56,090 --> 00:32:01,430
+acid اللي هو ال acid product هيربط بيه اليوزين and
+
+439
+00:32:01,430 --> 00:32:04,430
+methylene blue لأنه احنا قولنا هو as pH indicator
+
+440
+00:32:04,430 --> 00:32:10,490
+هيكون رابط ما بينهم تمام اسمها ال amide اللي هي
+
+441
+00:32:10,490 --> 00:32:16,040
+group هيعطي نلون metallic green sheetبتكون هذه
+
+442
+00:32:16,040 --> 00:32:19,400
+البكتيريا إذا على طبق ال amp اللي حاصة metallic
+
+443
+00:32:19,400 --> 00:32:24,560
+green chain بكون احتمال انها تكون ال E.coli لان ال
+
+444
+00:32:24,560 --> 00:32:28,340
+E.coli من الكوليفارم بتعمل fermentation للكتاز
+
+445
+00:32:28,340 --> 00:32:31,340
+بتكون أسد بقولي هذا الأسد بيرتبط بالإيوزين
+
+446
+00:32:31,340 --> 00:32:34,560
+والميثاليني بلو لكن الرابط ما بين الإيوزين
+
+447
+00:32:34,560 --> 00:32:39,400
+والميثاليني بلو بتكون رابط ما بينهم فبالتالي
+
+448
+00:32:39,400 --> 00:32:45,260
+بيعطيني اللون الأخضر ال metallic greenالـ Coliform
+
+449
+00:32:45,260 --> 00:32:47,960
+بكتيريا احنا قلنا E.coli, Klebsiella, Citrobacter,
+
+450
+00:32:48,080 --> 00:32:51,760
+Enterobacter الـ E.coli عرفنا انه اشي خاص في هاي
+
+451
+00:32:51,760 --> 00:32:54,100
+من عائلة الـ Lactose Fermenter بتعطي Metallic
+
+452
+00:32:54,100 --> 00:32:57,120
+Green Chain وعرفنا شو السبب طيب الـ Klebsiella
+
+453
+00:32:57,120 --> 00:32:59,160
+Citrobacter ال Enterobacter بقول بتعمل
+
+454
+00:32:59,160 --> 00:33:02,660
+fermentation للـ Lactose بتكون أسد زي الـ E.coli
+
+455
+00:33:02,660 --> 00:33:07,620
+بتربط بي اليوزين والميثيليني بلو بتعطي لون Pink to
+
+456
+00:33:07,620 --> 00:33:13,260
+Purple Colony أو Dark Purple Colonyلكن الـ E.coli
+
+457
+00:33:13,260 --> 00:33:16,820
+بتكون رابطة ما بين الإيزين والميسيليان بلو لكن هن
+
+458
+00:33:16,820 --> 00:33:20,720
+ما بتتكون رابطة فبنتج اللون البربل هن بتتكون رابطة
+
+459
+00:33:20,720 --> 00:33:23,540
+ما بين الإيزين والميسيليان بلو فبنتج اللون الـ
+
+460
+00:33:23,540 --> 00:33:26,940
+Metallic Green Sheen هذا بالنسبة للـ Lactose
+
+461
+00:33:26,940 --> 00:33:29,860
+Fermenter الـ Non-Lactose Fermenter بقول لي ما
+
+462
+00:33:29,860 --> 00:33:32,560
+بيصير اي fermentation للـ Lactose فبالتالي مش
+
+463
+00:33:32,560 --> 00:33:36,300
+هيتغير اللون ال media ومش هتتغير اللون الكوني
+
+464
+00:33:36,300 --> 00:33:41,410
+هتظلها colorless او ممكن انها تاخداللون الكلوني
+
+465
+00:33:41,410 --> 00:33:45,270
+لون ال media طبعا ممكن انها تكون colorless او ممكن
+
+466
+00:33:45,270 --> 00:33:48,250
+تاخد لون ال media لون ال media لون violet فاتح
+
+467
+00:33:48,250 --> 00:33:52,250
+فابدى افرق ما بين ال lactose و ال non lactose خاصة
+
+468
+00:33:52,250 --> 00:33:55,610
+انه ال lactose في المنتر بتكون dark purple لكن هاي
+
+469
+00:33:55,610 --> 00:34:00,070
+لو اخدت لون ال media بيكون لون violet فاتح طيب لو
+
+470
+00:34:00,070 --> 00:34:04,450
+طبق AMP لكن مالاقيت فيه اي نمو يبقى بعرف انه هي
+
+471
+00:34:04,450 --> 00:34:07,830
+high gram positive bacteria صار لها inhibition
+
+472
+00:34:08,070 --> 00:34:12,570
+بواسطة الصبغات الأيوزين والمثلين قبله هك اتعرفنا
+
+473
+00:34:12,570 --> 00:34:24,190
+او اتعرفنا على ال ال EMB نيجي لل slides EMB is a
+
+474
+00:34:24,190 --> 00:34:28,830
+primary differential media هي تعتبر differential
+
+475
+00:34:28,830 --> 00:34:32,390
+media وعرفنا لأن فيها ال lactose ك differential
+
+476
+00:34:32,390 --> 00:34:36,580
+agent however it does inhibit the growth ofsome of
+
+477
+00:34:36,580 --> 00:34:39,700
+a gram positive bacteria و اللي بتعمل inhibition
+
+478
+00:34:39,700 --> 00:34:43,920
+لـ some of a gram positive bacteria تمام فبالتالي
+
+479
+00:34:43,920 --> 00:34:48,560
+هي selection for a gram negative bacteria AMB is
+
+480
+00:34:48,560 --> 00:34:51,380
+used to differentiate between enteric lactose
+
+481
+00:34:51,380 --> 00:34:54,320
+fermenter الكوليفارم and non-lactose fermenter هي
+
+482
+00:34:54,320 --> 00:34:56,660
+بتفرق بين ال lactose fermenter و non-lactose
+
+483
+00:34:56,660 --> 00:34:59,200
+fermenter من عالة ال enterobacteriaceae اللي هي
+
+484
+00:34:59,200 --> 00:35:02,330
+الكوليفارم و ال non-coliformبالإضافة بتعمل
+
+485
+00:35:02,330 --> 00:35:05,850
+identification للـ E.coli لأن الـ E.coli بتعطيها
+
+486
+00:35:05,850 --> 00:35:10,350
+لون مميز لـ Metallic Green Chain فبالتالي بتساعد
+
+487
+00:35:10,350 --> 00:35:14,830
+في ال identification للـ E.coli فده بشكل خاص Iosin
+
+488
+00:35:14,830 --> 00:35:17,550
+Methylene Blue Agar بقولي هذه ال media الـ AMP
+
+489
+00:35:17,550 --> 00:35:22,850
+اللي هي Iosin Methylene Blue Agar content peptone
+
+490
+00:35:22,850 --> 00:35:27,380
+تحتوي علىتحتوي على اللاكتوز طبعا البيبتون كمصدر
+
+491
+00:35:27,380 --> 00:35:30,980
+للتغذية أي ميديا فيها بيبتون كمصدر للتغذية عشان
+
+492
+00:35:30,980 --> 00:35:35,160
+البكتيريا تنمو تتكثر اللاكتوز differential agent
+
+493
+00:35:35,160 --> 00:35:39,140
+الداي الأيوزين والميثاليني بلو تعتبر selective
+
+494
+00:35:39,140 --> 00:35:44,080
+agent واللي ال sugar provide هذا ال sugar هو ال
+
+495
+00:35:44,080 --> 00:35:49,400
+substrate عشان البكتيريا تعملي fermentationThe
+
+496
+00:35:49,400 --> 00:35:51,960
+dyes inhibit the growth of ground positive
+
+497
+00:35:51,960 --> 00:35:54,880
+bacteria organism قولنا إن الصبغات الميسيليني بلو
+
+498
+00:35:54,880 --> 00:35:57,360
+والايوزين بتعمل inhibition لـground positive
+
+499
+00:35:57,360 --> 00:36:02,000
+organism and under acidic condition also produce
+
+500
+00:36:02,000 --> 00:36:08,200
+dark purple بقولّي إذا أتكون عندي acidic product
+
+501
+00:36:08,200 --> 00:36:13,700
+يبقى هاي هتترتبط بالطبع المركب هيرتبط بالايوزين
+
+502
+00:36:13,700 --> 00:36:18,880
+والميسيليني بلو فبالتاليهتعطيني لون dark purple
+
+503
+00:36:18,880 --> 00:36:22,320
+يبقى ال dies بنفس الوقت مع ال under acidic
+
+504
+00:36:22,320 --> 00:36:24,900
+condition مع ال acidic condition لو اتكون عندي
+
+505
+00:36:24,900 --> 00:36:29,400
+acidic product هترتبط مع اللي هي هذا ال acidic
+
+506
+00:36:29,400 --> 00:36:33,740
+product هيرتبط مع الصفغات هيعطيني لون dark purple
+
+507
+00:36:33,740 --> 00:36:37,740
+فبالتالي يعتبر ال dies برضه as BH indicator ك
+
+508
+00:36:37,740 --> 00:36:42,220
+selective agent طيب
+
+509
+00:36:44,060 --> 00:36:48,060
+الأيوزين والميتليني بلو تجعل لكتوز فرمنتر تحصل على
+
+510
+00:36:48,060 --> 00:36:51,220
+لون بنك كولوني بقولي أن الأيوزين والميتليني بلو
+
+511
+00:36:51,220 --> 00:36:55,200
+تجعل لكتوز فرمنتر تحصل على لون بنك كولوني أو هي
+
+512
+00:36:55,200 --> 00:36:59,480
+بالظبط بتاعتي dark purple لش بتاعتي ال dark purple
+
+513
+00:36:59,480 --> 00:37:02,380
+قلنا بتكون أسد ال product هذا ال أسد ال product
+
+514
+00:37:02,380 --> 00:37:06,620
+برتبط مع هذه الصفقات فبعطيني ال بنك or dark purple
+
+515
+00:37:06,620 --> 00:37:11,940
+ال equalize قلنا إنه إلها إشي خاص تمامبقولي هذه
+
+516
+00:37:11,940 --> 00:37:15,300
+الصبغة بتترسب في الخلايا و بتعطي اللون الـ
+
+517
+00:37:15,300 --> 00:37:19,080
+metallic green chain يبقى الـ E.coli بتعطي الـ
+
+518
+00:37:19,080 --> 00:37:24,240
+metallic green chain تمام لكن ال other كل فرم غير
+
+519
+00:37:24,240 --> 00:37:28,080
+ال E.coli بتعطي اللون ال dark purple و عرفنا ليش
+
+520
+00:37:28,080 --> 00:37:30,800
+بتعطي اللون ال metallic green chain لأنه بتكون
+
+521
+00:37:30,800 --> 00:37:33,960
+رابط ما بين اليوزين والميثالين blueطب الـ non
+
+522
+00:37:33,960 --> 00:37:36,740
+-lactose fermenter بقولي هتضلها colorless لأنه ما
+
+523
+00:37:36,740 --> 00:37:39,500
+هيصير fermentation أو ممكن تاخد لون ال media اللي
+
+524
+00:37:39,500 --> 00:37:43,740
+هو اللون ال violet الفاتح زي السلمونيلا بقولي
+
+525
+00:37:43,740 --> 00:37:47,560
+السلمونيلا تعتبر non-lactose fermenter لو نميتها
+
+526
+00:37:47,560 --> 00:37:50,660
+على ال A&B ممكن تكون colorless و ممكن تاخد لون ال
+
+527
+00:37:50,660 --> 00:37:54,800
+media طبعا هي واحدة من أهم المسببات في ال food
+
+528
+00:37:54,800 --> 00:37:57,120
+poisoning في التسمم الغذائي
+
+529
+00:37:59,590 --> 00:38:03,050
+طيب بالنسبة لها هاي ال media used to confirm the
+
+530
+00:38:03,050 --> 00:38:05,670
+presence of E.coli in water sample contaminated
+
+531
+00:38:05,670 --> 00:38:09,830
+with sewage or فيه كالماتيريا ولهاي ال media بشكل
+
+532
+00:38:09,830 --> 00:38:12,550
+خاص مش كمان يتفرق بين ال lactose و ال non-lactose
+
+533
+00:38:12,550 --> 00:38:15,510
+لأنه هنتعرف في ميدية ما كمان يتميز بين ال lactose
+
+534
+00:38:15,510 --> 00:38:19,250
+و ال non-lactose و فعلا بتعطي ألوان واضحة لكن هاي
+
+535
+00:38:19,250 --> 00:38:23,630
+ال media بشكل خاص تستخدم عشان أؤكد وجود ال E.coli
+
+536
+00:38:23,630 --> 00:38:28,190
+في عينات ال water sample contaminated with sewage
+
+537
+00:38:29,020 --> 00:38:33,600
+ليش هاي تستخدم لأن الـ E coli قلنا في هذه ال media
+
+538
+00:38:33,600 --> 00:38:37,140
+ابتعدي لون مميز ابتعدي لون metallic green chain
+
+539
+00:38:37,140 --> 00:38:42,680
+فبالتالي هي بتأكد وجود ال E coli you will use this
+
+540
+00:38:42,680 --> 00:38:46,380
+agar again within water sampling experiment to
+
+541
+00:38:46,380 --> 00:38:49,440
+differentiate between E coli and enterobacter
+
+542
+00:38:49,440 --> 00:38:53,580
+بقوللي في هذه ال media بميز بين ال E coli و ال
+
+543
+00:38:53,580 --> 00:38:58,740
+enterobacter على ال AMP طيب ليش بتميزفي هذه
+
+544
+00:38:58,740 --> 00:39:03,100
+الميديا بين الـ E.coli و الـ Enterobacter الـ E
+
+545
+00:39:03,100 --> 00:39:06,280
+.coli تنتين يعتبروا lactose fermenter لكن الـ E
+
+546
+00:39:06,280 --> 00:39:10,000
+.coli قلنا بينتج acid بيرتبط مع الـ E.osin و
+
+547
+00:39:10,000 --> 00:39:13,660
+الميثيلين blue بتكون رابطة بين الـ E.osin و
+
+548
+00:39:13,660 --> 00:39:16,900
+الميثيلين blue فبيعطيني اللون الـ metallic green
+
+549
+00:39:16,900 --> 00:39:22,500
+الشين بالنسبة للـ Enterobacter هذه اسمها fish eye
+
+550
+00:39:22,500 --> 00:39:27,020
+الـ Enterobacter ملاحظ أن شكلها زي الـ fish eye
+
+551
+00:39:27,810 --> 00:39:32,610
+عارفين بتعمل fermentation لل lactose تمام بتعمل
+
+552
+00:39:32,610 --> 00:39:36,470
+fermentation لل lactose sorry بتعمل fermentation
+
+553
+00:39:36,470 --> 00:39:42,310
+لل lactose بتكون acid هذا ال acid بيرسبت مع الصبغة
+
+554
+00:39:42,310 --> 00:39:48,010
+تمام بيترسب في المنتصف الصبغة بتترسب في المنتصف
+
+555
+00:39:48,520 --> 00:39:52,120
+عالطرف بيكون في عندي colorless فبالتالي بلاحظ أنه
+
+556
+00:39:52,120 --> 00:39:56,200
+هي زي عين السبقة .. زي عين السمكة يبقى السبقة
+
+557
+00:39:56,200 --> 00:40:00,080
+بتترسب في منتصف ال colony على الاتراف بحيث أن ال
+
+558
+00:40:00,080 --> 00:40:04,720
+colony colorless يبقى بتكون زي عين السبقة فش .. فش
+
+559
+00:40:04,720 --> 00:40:09,120
+eye فبالتالي هيك فرقات بين ال equal eye و ال
+
+560
+00:40:09,120 --> 00:40:15,570
+interior بأكترهذا بالنسبة للميديا
+
+561
+00:40:15,570 --> 00:40:17,310
+اللي استخدمه بالتفريق بين الـ E.coli و الـ
+
+562
+00:40:17,310 --> 00:40:20,090
+Enterobacter لأن الـ E.coli بتدّي متاليكا جرينتشين
+
+563
+00:40:20,090 --> 00:40:23,810
+و عرفنا شو السبب الـ Enterobacter بتعطي fish eye
+
+564
+00:40:23,810 --> 00:40:27,550
+لأنه بتعمل fermentation للـ Lactose بيرتبط مع
+
+565
+00:40:27,550 --> 00:40:32,350
+الصبغة بتترثب الصبغة في النص على الطرف بحيث انه في
+
+566
+00:40:32,350 --> 00:40:36,010
+اندي colorless فبالتالي بتكون زي عين الصبغة هيك
+
+567
+00:40:36,010 --> 00:40:37,550
+بنكون خلصنا نيجي ل
+
+568
+00:40:45,100 --> 00:40:50,340
+لسلايدات للصور هان ملاحظ طبق a and b نمت عليه
+
+569
+00:40:50,340 --> 00:40:54,160
+البكتيريا يبقى هي إجرام negative بكتيريا تمام ظهر
+
+570
+00:40:54,160 --> 00:40:56,880
+اللون الأخضر يبقى بحكي أنه والله هي احتمال انها
+
+571
+00:40:56,880 --> 00:41:01,020
+تكون E.coli وعرفنا ليش اتكون اللون الأخضر بالنسبة
+
+572
+00:41:01,020 --> 00:41:07,140
+لهان fish eye colony ملاحظ انهالصبغة اترسبت في
+
+573
+00:41:07,140 --> 00:41:09,660
+المنتصف وعلى الطرف فى عندي colorless يبقى هي
+
+574
+00:41:09,660 --> 00:41:13,580
+احتمال انها تكون enterobacter السبب انه صار فى
+
+575
+00:41:13,580 --> 00:41:17,400
+عندي fermentation للكتاز اتكون acid ارتبط مع
+
+576
+00:41:17,400 --> 00:41:22,480
+الصبغة ارتبط مع الصبغة فبالتالي كونلي لون dark
+
+577
+00:41:22,480 --> 00:41:26,100
+purple اترسبت الصبغة في المنتصف وعلى الطرف كان فى
+
+578
+00:41:26,100 --> 00:41:30,010
+عندي colorless يبقى هى enterobacterطبعاً باقي اللي
+
+579
+00:41:30,010 --> 00:41:33,950
+هي الكليبسيلة والستروبكتر بتعطي لون dark زي هذا
+
+580
+00:41:33,950 --> 00:41:38,070
+لكن ما بتترسف بالمنتصف الكليبسيلة والستروبكتر
+
+581
+00:41:38,070 --> 00:41:43,230
+بروتياس وسلمونيلا نفس الاشي بروتياس وسلمونيلا non
+
+582
+00:41:43,230 --> 00:41:46,730
+-lactose fermenter فبالتالي مش هيتغير اللون
+
+583
+00:41:46,730 --> 00:41:51,270
+الكولوني هتظلها لونها colorless او ممكن انها تاخد
+
+584
+00:41:51,270 --> 00:41:54,030
+اللون ال media اللي هو اللون اللي هو ال violet
+
+585
+00:41:54,030 --> 00:41:58,530
+الفاتحبالنسبة لـ Staphylococcus aureus لـ
+
+586
+00:41:58,530 --> 00:42:02,510
+Staphylococcus aureus على طبق الـ AMP المفترض ما
+
+587
+00:42:02,510 --> 00:42:05,050
+إنها تنمو لأنه هي gram positive بكتيريا يبقى لو
+
+588
+00:42:05,050 --> 00:42:08,830
+جبنا في الامتحان AMP ولاحظنا إنه والله في عندي طبق
+
+589
+00:42:08,830 --> 00:42:13,650
+ما في عندي أي نمو على الطبق يبقى بحكي إنه هاي ال
+
+590
+00:42:13,650 --> 00:42:17,670
+bacteria صار لها inhibition بسبواسطة اللي هي
+
+591
+00:42:17,670 --> 00:42:20,690
+selective agent اليوزينوي الميتليني بلو يبقى
+
+592
+00:42:20,690 --> 00:42:24,570
+احتمال إنها تكون gram positive بكتيرياهذا بالنسبة
+
+593
+00:42:24,570 --> 00:42:31,150
+لـ ال .. اللي هو ال .. شو تفسير الألوان طبعا الـ
+
+594
+00:42:31,150 --> 00:42:35,010
+AMP الـ lactose fermenter لون dark purple لكن الـ
+
+595
+00:42:35,010 --> 00:42:38,530
+E.coli فقط الـ E.coli بتعطي metallic green chain
+
+596
+00:42:38,530 --> 00:42:42,030
+الـ interior بكتر بمعيزها الـ fish eye colony الـ
+
+597
+00:42:42,030 --> 00:42:44,610
+non lactose fermenter بتضلها colorless أو ممكن
+
+598
+00:42:44,610 --> 00:42:49,410
+تاخد لون ال media لون ال .. الوسط أي إجراء
+
+599
+00:42:49,410 --> 00:42:52,540
+positive بكتيريا مش هتنمو على هذا الوسطالـ E.coli
+
+600
+00:42:52,540 --> 00:42:56,040
+من اللاحظ أنه والله هان هي lactose fermented
+
+601
+00:42:56,040 --> 00:43:01,580
+وعرفنا شو سبب تكون اللون الأخضر على ال A&B لـ E
+
+602
+00:43:01,580 --> 00:43:07,840
+.coli هان الـ Enterobacter باخد اللون ال pink تمام
+
+603
+00:43:07,840 --> 00:43:13,660
+على ال A&B احنا قلنا pink or dark purple يبقى هان
+
+604
+00:43:13,660 --> 00:43:18,050
+من اللاحظ أنه احنا مش هنشوفطبعا ال fish eye colony
+
+605
+00:43:18,050 --> 00:43:22,390
+انترو باكتر المفترض انها تكون fish eye colony ليش
+
+606
+00:43:22,390 --> 00:43:26,070
+مش هنشوف هاي ال fish eye colony لأنه هاي مش
+
+607
+00:43:26,070 --> 00:43:29,470
+isolated colony ما زلعت بطريقة الأربع عشان أحصل
+
+608
+00:43:29,470 --> 00:43:32,990
+على isolated colony فبالتالي مش هشوف ال fish eye
+
+609
+00:43:32,990 --> 00:43:35,390
+colony عشان أشوف ال fish eye colony لازم أزرع
+
+610
+00:43:35,390 --> 00:43:41,590
+بطريقة الأربع طيب هيك خلصنا ال A&B نيجي لآخر media
+
+611
+00:43:41,590 --> 00:43:46,120
+اللي هي الماكمكي أجارالمكونكي زي الـ «AMP»
+
+612
+00:43:46,120 --> 00:43:48,860
+«Selection» لـ «Gram Negative Bacteria» يبقى لـ
+
+613
+00:43:48,860 --> 00:43:52,180
+«Gram Positive Bacteria» هيصير لها Inhibition مين
+
+614
+00:43:52,180 --> 00:43:55,460
+الـ «Selective Agent»؟ الـ «Selective Agent» نفس
+
+615
+00:43:55,460 --> 00:44:00,400
+هذه الميديا الـ «AMP» برضه يعتبر «Dyes» لكن بيختلف
+
+616
+00:44:00,400 --> 00:44:03,880
+«Dyes» و «Salt» «Crystal Violet» and «Bi Salt»
+
+617
+00:44:03,880 --> 00:44:08,680
+بيتفق مع ال «AMP» أنه والله «Salt» مع ال «AMP»
+
+618
+00:44:08,680 --> 00:44:12,710
+أنها «Dyes»لكن هناك كان المثلين blue والايوزين
+
+619
+00:44:12,710 --> 00:44:17,230
+والايوزين كانت هناك لكن hand ل crystal violet
+
+620
+00:44:17,230 --> 00:44:21,330
+تعتبر ال by salt يعتبر hand salt يبقى في هذه
+
+621
+00:44:21,330 --> 00:44:24,330
+الميديا selective agent dies and salt crystal
+
+622
+00:44:24,330 --> 00:44:27,770
+violet dies وال by salt يعتبر salt طيب
+
+623
+00:44:27,770 --> 00:44:30,450
+differential agent نفس ال A and B differential
+
+624
+00:44:30,450 --> 00:44:34,030
+agent في هذه الميديا lactose لكن في ال manitol
+
+625
+00:44:34,030 --> 00:44:39,520
+salt أجار كان manitolهتميز ليه نفس الشيباق بين الـ
+
+626
+00:44:39,520 --> 00:44:42,480
+coliform و الـ non-coliform بكتيريا هذه صح بتميز
+
+627
+00:44:42,480 --> 00:44:45,340
+بين الـ coliform و الـ non-coliform لكن بشكل خاص
+
+628
+00:44:45,340 --> 00:44:49,300
+بتميز الـ E-coli تعملي identification للـ E-coli و
+
+629
+00:44:49,300 --> 00:44:53,640
+كمان للـ enterobacter لأنه بتدي فش I كله الـ pH
+
+630
+00:44:53,640 --> 00:44:58,520
+indicator الـ neutral red هذا جديد ممر معناه الـ
+
+631
+00:44:58,520 --> 00:45:01,880
+neutral red نفس الـ methyl red اللي أخدناه تبحث
+
+632
+00:45:01,880 --> 00:45:06,790
+الـ methyl redعكس البيئة اللي فيه نار رد في الاسد
+
+633
+00:45:06,790 --> 00:45:10,190
+بيكون لونه رد وفي الباس بيكون لونه يلو لـ Gram
+
+634
+00:45:10,190 --> 00:45:13,510
+Negative بكتيريا فقط اللي هي هتميز في هذه الميديا
+
+635
+00:45:13,510 --> 00:45:16,770
+هتميز لبعض الـ Lactose Fermenter و لبعض الـ Non
+
+636
+00:45:16,770 --> 00:45:20,010
+Lactose Fermenter اللاكتوز فرمنتر هتعمل
+
+637
+00:45:20,010 --> 00:45:23,190
+fermentation لللاكتوز هيتكوّن اسد بما أنه فيه ND
+
+638
+00:45:23,190 --> 00:45:27,310
+BH Indicator هيعمل على خفض ال BH هيتكوّن لون pink
+
+639
+00:45:27,310 --> 00:45:30,720
+to red كله لكن الـ Non Lactose Fermenterمش هتعمل
+
+640
+00:45:30,720 --> 00:45:33,240
+fermentation للـ lactose هيدلوا لون الـ colony
+
+641
+00:45:33,240 --> 00:45:37,120
+colorless لـ Gram-positive بكتيريا مش هيصيرلها أي
+
+642
+00:45:37,120 --> 00:45:43,780
+نمو زي الـ AMP فبالتالي مش هتنمو على الطبق المكون
+
+643
+00:45:43,780 --> 00:45:47,020
+K agar used to isolate Gram-negative بكتيريا قلنا
+
+644
+00:45:47,020 --> 00:45:49,960
+لأنه هي بتنمي لـ Gram-negative بكتيريا هي تعتبر
+
+645
+00:45:49,960 --> 00:45:53,540
+selective و differential media less selective لأنه
+
+646
+00:45:53,540 --> 00:45:56,620
+لـ Gram-positive بكتيريا بيصيرلها Inhibition من
+
+647
+00:45:56,620 --> 00:45:59,560
+الـ Selective agent الـ bisalt and crystal violet
+
+648
+00:46:00,070 --> 00:46:03,250
+طيب الـ Gram Negative بكتيريا فقط اللي هي اللي
+
+649
+00:46:03,250 --> 00:46:06,690
+هتنمو طيب ديفرانشيات ليش هي differential media
+
+650
+00:46:06,690 --> 00:46:10,190
+لأنها بتفرق بين الـ Gram Negative بكتيريا وقدرتها
+
+651
+00:46:10,190 --> 00:46:14,410
+إنها تعمل fermentation للـ Lactose بالنسبة للـ
+
+652
+00:46:14,410 --> 00:46:16,770
+Coliform طبعا هي بتميز الـ Coliform و الـ Non
+
+653
+00:46:16,770 --> 00:46:19,910
+-coliform نيجي للـ Coliform بسيلاي بتكون acid
+
+654
+00:46:19,910 --> 00:46:23,910
+نتيجة الـ Lactose fermentation فبالتالي بتخلي لون
+
+655
+00:46:23,910 --> 00:46:29,660
+الكولوني يتحول للون أحمر نتيجة خفض الـ pHبقولي هنا
+
+656
+00:46:29,660 --> 00:46:33,700
+في الـ E.coli طبعا تعتبر من الكوليفارم بكتيريا زي
+
+657
+00:46:33,700 --> 00:46:36,500
+ما ال E.coli كانت في ال A وB كانت مميزة باللون
+
+658
+00:46:36,500 --> 00:46:41,500
+الأخضر هنا برضه مميزة في المقنكي بقولي بتنتج
+
+659
+00:46:41,500 --> 00:46:46,940
+produce even greater quantities of acid causing
+
+660
+00:46:46,940 --> 00:46:50,260
+the surrounding media and the colonists to turn
+
+661
+00:46:50,260 --> 00:46:56,120
+red بقولي بتنتج كمية كبيرة من الأسد فبالتاليحوالين
+
+662
+00:46:56,120 --> 00:46:59,120
+اللي هو الـ Media الـ Colony نفسها والـ Media
+
+663
+00:46:59,120 --> 00:47:02,900
+بيصير لونها أحمر تمام هذا اللي بيميز الـ E.coli
+
+664
+00:47:02,900 --> 00:47:07,240
+أنه بلاحظ مش بس الـ Surrounding Media كمان الـ
+
+665
+00:47:07,240 --> 00:47:12,520
+Colony اتحول لونها للون أحمر نتيجة اللي هو الكمية
+
+666
+00:47:12,520 --> 00:47:15,680
+الكبيرة من الأسد اللي نتج من عملية ال fermentation
+
+667
+00:47:15,680 --> 00:47:19,740
+يبقى هذا بيميز الـ E.coli في المكونكي في الـ AMP
+
+668
+00:47:19,740 --> 00:47:23,570
+بتحول اللون للون أخضرالـ Non-Coliform Basilaria أو
+
+669
+00:47:23,570 --> 00:47:26,030
+الـ Non-Lactose Fermenter ما بيصير fermentation
+
+670
+00:47:26,030 --> 00:47:29,950
+للـ Lactose فبالتالي بتظلها transparent or
+
+671
+00:47:29,950 --> 00:47:34,610
+colorless لـ Gram Positive بكتيريا اتفقنا أي ميديا
+
+672
+00:47:34,610 --> 00:47:38,030
+بتعمل inhibition لأي نوع من أنواع البكتيريا ما
+
+673
+00:47:38,030 --> 00:47:41,510
+بيصير kill ما بتموت في هذه الميديا فقط بيصير لـ
+
+674
+00:47:41,510 --> 00:47:48,560
+growth inhibitionطيب نيجي للصور طبق مقنكي لاحظنا
+
+675
+00:47:48,560 --> 00:47:51,280
+فيه انه يبقى الـBacteria Gram Negative Bacteria طب
+
+676
+00:47:51,280 --> 00:47:54,040
+بدني أميز هل هي lactose fermenter or non-lactose
+
+677
+00:47:54,040 --> 00:47:59,840
+fermenter الـ E.coli قولنا إنه بتحول لون الوسط
+
+678
+00:47:59,840 --> 00:48:03,540
+وكمان الكلوني للون الزهر نتيجة إنتاج كمية كبيرة من
+
+679
+00:48:03,540 --> 00:48:08,000
+الأسد فبالتالي عمل على حفظ الـ pH وتحول لون الوسط
+
+680
+00:48:08,000 --> 00:48:14,450
+للون اللي هو أحمر هذا بالنسبة لـ E.coli تمامالـ
+
+681
+00:48:14,450 --> 00:48:17,210
+Enterobacter نفس الـ E.coli الـ E.coli و الـ
+
+682
+00:48:17,210 --> 00:48:19,090
+Enterobacter و الـ Klebsiella و الـ Citrobacter
+
+683
+00:48:19,090 --> 00:48:21,890
+نفس الإيش بتاعته لون pink to red لكن اللي بيفرق
+
+684
+00:48:21,890 --> 00:48:25,310
+الـ E.coli إنها من لاحظ الـ surrounding media و
+
+685
+00:48:25,310 --> 00:48:30,350
+الكولوني لونها أحمر تمام بالنسبة للـ Enterobacter
+
+686
+00:48:30,350 --> 00:48:36,330
+برضه نمت لاحظت أن اللون pink to red يبقى من عائلة
+
+687
+00:48:36,330 --> 00:48:40,910
+الـ coliform لكتوز أو نحكي لكتوز fermented
+
+688
+00:48:40,910 --> 00:48:45,450
+برودياسوي السلمونيلاتنتهى الـ Non-lactose
+
+689
+00:48:45,450 --> 00:48:47,870
+fermentation يبقى مش هتعمل fermentation للاكتوز
+
+690
+00:48:47,870 --> 00:48:51,990
+هتظهر اللون الكلوني لونها colorless or transparent
+
+691
+00:48:51,990 --> 00:48:55,330
+الاستفيلة كوكس أورياسمين لـ Gram-positive bacteria
+
+692
+00:48:55,330 --> 00:48:58,570
+فبالتالي مش هلاقي أي نمو على الطواق لأن هاي ال
+
+693
+00:48:58,570 --> 00:49:01,970
+media بيصير فيها inhibition لـ Gram-positive
+
+694
+00:49:01,970 --> 00:49:07,210
+bacteria طيب لو سألنا طبعا هان هي معنى ماد نتيجة
+
+695
+00:49:07,210 --> 00:49:11,170
+انه صار Inhibition بواسطة ال crystal violet و ال
+
+696
+00:49:11,170 --> 00:49:18,670
+bile saltلو سألنا سؤال إن الصدوموناس الصدوموناس
+
+697
+00:49:18,670 --> 00:49:22,470
+على طبق الـ AMP أو طبق المكونكي طبعاً هي الـ Gram
+
+698
+00:49:22,470 --> 00:49:26,350
+Negative Bacteria فأكيد الصدوموناس هتنمو على الـ
+
+699
+00:49:26,350 --> 00:49:30,130
+AMP والمكونكي وإحنا قلنا تنتين مش بس للـ
+
+700
+00:49:30,130 --> 00:49:32,610
+Enterobacteriasis هي بتنمي كل Gram Negative
+
+701
+00:49:32,610 --> 00:49:36,090
+Bacteria فعشان هيك لازم أعمل فحوصات فحوصات الـ
+
+702
+00:49:36,090 --> 00:49:39,340
+Oxidase فحوصات الـ Gelicose Fermentationعشان اتأكد
+
+703
+00:49:39,340 --> 00:49:42,400
+ان هي من عائلة الانتيرو بكتيريازي وبعدين احكي هل
+
+704
+00:49:42,400 --> 00:49:45,700
+هي من عائلة الكوليفارم او من الـnon-coliform
+
+705
+00:49:45,700 --> 00:49:49,600
+السوداموناس تعتبر اجرام نجатив بكتيريا اكيد هتنمو
+
+706
+00:49:49,600 --> 00:49:55,140
+على طبق المكونكي او ال AMP طيب ايش هتعطي كولوني؟
+
+707
+00:49:55,140 --> 00:49:58,640
+السوداموناس بالاصل هي non-gelicose fermenter
+
+708
+00:49:58,640 --> 00:50:02,800
+فبالتالي هي non-lactose بما انه ماعملة جلد
+
+709
+00:50:02,800 --> 00:50:05,540
+fermentation لأبسط سكر فبالتالي مش هتعمل
+
+710
+00:50:05,540 --> 00:50:10,240
+fermentation لالـ Dysaccharide اللاكتاز فبالتالي
+
+711
+00:50:10,240 --> 00:50:14,100
+تعتبر non-lactose fermenter يبقى على طبق الميكونكي
+
+712
+00:50:14,100 --> 00:50:18,280
+وعلى طبق ال A&B ال Sodomonas هتعامل معاملة ال non
+
+713
+00:50:18,280 --> 00:50:22,140
+-lactose fermenter هيكون على الميكونكي colorless
+
+714
+00:50:22,140 --> 00:50:25,960
+على ال A&B ممكن انها تكون colorless وممكن تاخد لون
+
+715
+00:50:25,960 --> 00:50:31,400
+الميدى هذا بالنسبة لل Sodomonas فاكما نكون خلصنا
+
+716
+00:50:32,240 --> 00:50:34,460
+معمل الـ «Selective» والـ «Differential Media»
+
+717
+00:50:34,460 --> 00:50:37,740
+تعرفنا على 3 ميديات مكون K, A, M, B مانيتال،
+
+718
+00:50:37,740 --> 00:50:40,220
+asphalt، أجار، كل ميديا من الـ «Selective» من الـ
+
+719
+00:50:40,220 --> 00:50:43,980
+«Differential Agent» أش بتعطي كيف بتفرق بين مين
+
+720
+00:50:43,980 --> 00:50:47,480
+ومين، كل مثلا الـ «Lactose Fermenter»، الـ «Non
+
+721
+00:50:47,480 --> 00:50:51,880
+-Lactose Fermenter»، شو اللون في كل حالة، كذلك في
+
+722
+00:50:51,880 --> 00:50:54,420
+المانيتال، اللي هي المانيتال الـ «Fermenter» والـ
+
+723
+00:50:54,420 --> 00:50:57,560
+«Non-Mannitol Fermenter» في كل منهم أش بتعطي لون
+
+724
+00:50:58,440 --> 00:51:03,920
+بأتمنى الأمور والشرح يكون واضح يعطيكم العافية
+
+725
+00:51:03,920 --> 00:51:06,540
+والسلام عليكم ورحمة الله وبركاته
+
diff --git a/PL9fwy3NUQKwZWgAb_V1ATlRRsH-6i3cPC/XZSMgPWLPeQ_postprocess.srt b/PL9fwy3NUQKwZWgAb_V1ATlRRsH-6i3cPC/XZSMgPWLPeQ_postprocess.srt
new file mode 100644
index 0000000000000000000000000000000000000000..c9e82dc0b8139251d59cd0666b7c7849c8ad13f6
--- /dev/null
+++ b/PL9fwy3NUQKwZWgAb_V1ATlRRsH-6i3cPC/XZSMgPWLPeQ_postprocess.srt
@@ -0,0 +1,1156 @@
+1
+00:00:00,390 --> 00:00:04,130
+السلام عليكم ورحمة الله وبركاته يعطيكم العافية في
+
+2
+00:00:04,130 --> 00:00:07,130
+هذا اللقاء هيكون معملنا بعنوان introduction to the
+
+3
+00:00:07,130 --> 00:00:10,910
+oil emerging compound microscope هنتعرف على ال
+
+4
+00:00:10,910 --> 00:00:14,370
+light compound microscope على المشهر الضوئي المركب
+
+5
+00:00:14,760 --> 00:00:18,700
+استخدامات هذا المجهر و أجزاءه و وظيفة كل جزء
+
+6
+00:00:18,700 --> 00:00:22,740
+بالتفصيل سنتعرف كمان على بعض النقاط المهمة بالنسبة
+
+7
+00:00:22,740 --> 00:00:26,480
+للـ Light Compound Microscope أخر جزئية هنتكلم كيف
+
+8
+00:00:26,480 --> 00:00:31,660
+أنا بدي أحفظ أو أني أحافظ على الـ Microscope و أني
+
+9
+00:00:31,660 --> 00:00:36,760
+أنظفه بعد ما أخلص من شغلفي الـ introduction عرفنا
+
+10
+00:00:36,760 --> 00:00:39,420
+الـ microbiology وقلنا it is the study of
+
+11
+00:00:39,420 --> 00:00:43,500
+microorganism that cannot be seen by next eye من
+
+12
+00:00:43,500 --> 00:00:46,220
+الـ microorganism اللي احنا هيتعامل معها في هذا
+
+13
+00:00:46,220 --> 00:00:50,800
+المثاق اللي هو البكتيريا حجم البكتيريا بيتراوح و
+
+14
+00:00:50,800 --> 00:00:54,000
+معظم حجم البكتيريا بيتراوح ما بين نص لاتنين micro
+
+15
+00:00:54,000 --> 00:00:58,560
+meter micro meter كتير حجم صغير عشان اني انا اقدر
+
+16
+00:00:58,560 --> 00:01:01,920
+اشوفها بحتاج ال microscope فانا بدي اعرف ال
+
+17
+00:01:01,920 --> 00:01:05,800
+microscopeMicroscopy is the technology of making
+
+18
+00:01:05,800 --> 00:01:09,000
+very small things visible to the human eye هو
+
+19
+00:01:09,000 --> 00:01:12,100
+التقنية باستخدامها في تكبير الأشياء الصغيرة التي
+
+20
+00:01:12,100 --> 00:01:16,340
+لا ترى بالعين المجردة used in the diagnosis of
+
+21
+00:01:16,340 --> 00:01:19,640
+many diseases يستخدم الميكروسكوفي تشخيص العديد من
+
+22
+00:01:19,640 --> 00:01:24,120
+الأمراض على سبيل المثال الـ Cancerفي معمل الـ
+
+23
+00:01:24,120 --> 00:01:29,320
+histopathology في معمل الانسجار كنتوا تاخدوا ال
+
+24
+00:01:29,320 --> 00:01:33,220
+biopsy و يتقطعوها و يتمرروها بميراحل و بعدين
+
+25
+00:01:33,220 --> 00:01:36,920
+تصبغوها و بعد كده تشوفوها تحت ال microscope كنتوا
+
+26
+00:01:36,920 --> 00:01:41,260
+تتأكدوا من عدم وجود نمو غير طبيعي للخلايا فبالتالي
+
+27
+00:01:41,260 --> 00:01:45,100
+انكوا تقدروا تشخصوا هل عند المريض cancer أو لأ
+
+28
+00:01:45,100 --> 00:01:51,630
+used for soil, insect and rock تستخدم في التربةوفي
+
+29
+00:01:51,630 --> 00:01:55,210
+الـ insect للحشرات لو أنا بدي أشوف حشر أشوف
+
+30
+00:01:55,210 --> 00:01:59,530
+التفاصيل طبعتها بشوفها تحت ال microscope بالنسبة
+
+31
+00:01:59,530 --> 00:02:04,150
+للسخور المجاهر بنستخدمها في تحليل التركيب المعدني
+
+32
+00:02:04,150 --> 00:02:08,190
+لبعض السخور used in pathological laboratories and
+
+33
+00:02:08,190 --> 00:02:12,010
+medical بنستخدم ال microscope في المختبرات طبعا
+
+34
+00:02:12,010 --> 00:02:16,540
+أكيد مافي أي مختبر مافي موجود microscopeلو انا
+
+35
+00:02:16,540 --> 00:02:20,880
+مثلا بدي اعمل فيلم للدم بصفغه بصبخات خاصة وبشوفه
+
+36
+00:02:20,880 --> 00:02:24,960
+تحت ال microscope من خلاله هيتم تشخيص العديد من
+
+37
+00:02:24,960 --> 00:02:28,560
+أمراض الدم المختلفة من الانيميا واللوكيميا
+
+38
+00:02:28,560 --> 00:02:32,520
+industrial microscope used for metals and various
+
+39
+00:02:32,520 --> 00:02:37,080
+kinds of measurements نستخدم ال microscope للمعادن
+
+40
+00:02:37,480 --> 00:02:41,100
+educational microscope used for research أكيد لأ
+
+41
+00:02:41,100 --> 00:02:46,860
+هدف تعليمي وأيضا في مجار الأبحاث وفي رؤية تركيب
+
+42
+00:02:46,860 --> 00:02:51,540
+الخلايا النباتية والحيوانية وغيرها من التطبيقات
+
+43
+00:02:51,540 --> 00:02:56,220
+bacteria is one of the small microscope that
+
+44
+00:02:56,220 --> 00:02:58,540
+cannot be seen with the naked eye اتفقنا أن
+
+45
+00:02:58,540 --> 00:03:01,260
+البكتيريا من الكائنات الدقيقة التي لا ترى بالعين
+
+46
+00:03:01,260 --> 00:03:01,980
+المجردة
+
+47
+00:03:04,330 --> 00:03:09,850
+معظم حجم البكتيريا يتراوح بين نصف لإتنين ميكرو متر
+
+48
+00:03:09,850 --> 00:03:14,110
+وهذا اتفقنا عليه في أول المعمل لذلك يحتاج الناس
+
+49
+00:03:14,110 --> 00:03:17,530
+لمجموعة البكتيريا للوقت السفيري باستخدام ميكروسكوب
+
+50
+00:03:17,530 --> 00:03:22,050
+لرؤيه، عشان هيك أنا بحتاج الميكروسكوب حتى أني أنا
+
+51
+00:03:22,050 --> 00:03:27,170
+أكبرها عشان أقدر أشوفها لكن أكيد احنا قلنا إن معظم
+
+52
+00:03:27,170 --> 00:03:30,570
+حجم البكتيريا بيتراوح ما بين نصف لإتنين ميكرو متر
+
+53
+00:03:31,070 --> 00:03:34,630
+أكيد لكل قاعدة شواز في علم الـ Microbiology مافي
+
+54
+00:03:34,630 --> 00:03:39,050
+اشي Absolute مافي اشي مطلق كل اشي له استثناء من
+
+55
+00:03:39,050 --> 00:03:42,410
+هذه الاستثناء ان انا في عيني Giant Bacteria اسمها
+
+56
+00:03:42,410 --> 00:03:47,750
+Thiomargarita Namibiansi هذه البكتيريا تعتبر Giant
+
+57
+00:03:47,750 --> 00:03:51,970
+Bacteria بقدر اشوفها في عيني المجردة بيتراوح حجم
+
+58
+00:03:51,970 --> 00:03:55,910
+هذه البكتيريا ما بين 1 من 10 ل 3 من 10 ملي متر لو
+
+59
+00:03:55,910 --> 00:04:00,770
+انا بدي حوّلها ل micro meterتروح ما بين 100 لـ 300
+
+60
+00:04:00,770 --> 00:04:04,630
+ميكرو متر في الـ Diameter وبعض الأحيان ممكن توصل
+
+61
+00:04:04,630 --> 00:04:10,870
+لـ 75 من 100 ميلي متر أي 750 ميكرو متر يبقى جملة
+
+62
+00:04:10,870 --> 00:04:14,310
+كل البكتيريا لتراب العين المجرة ده غلط لأنه أنا
+
+63
+00:04:14,310 --> 00:04:17,430
+فيها عند ستنقة فيها عندي تيو مارجاريتا نامي
+
+64
+00:04:17,430 --> 00:04:20,730
+بيانسيا هنالك مختلفة نوعات من الميكروسكوب التي
+
+65
+00:04:20,730 --> 00:04:23,810
+تستخدمها في حياة الميكروبيا وقلنا إنه احنا بنعرف
+
+66
+00:04:23,810 --> 00:04:27,250
+أنواع مختلفة من الميكروسكوب على سبيل المثال أنا
+
+67
+00:04:27,250 --> 00:04:30,210
+فيه عنديالـ Light Microscope يعني دي الـ Light
+
+68
+00:04:30,210 --> 00:04:32,830
+Compound Microscope يعني دي الـ Fluorescent الـ
+
+69
+00:04:32,830 --> 00:04:36,090
+Electron Microscope كل هذه الميكروسكوبات أنا
+
+70
+00:04:36,090 --> 00:04:42,110
+بستخدمها في علم الـ Microbiology لكن in this lab
+
+71
+00:04:42,110 --> 00:04:44,890
+you will become familiar with the use of Light
+
+72
+00:04:44,890 --> 00:04:48,390
+Compound Microscope فهذا المعمل هنتعرف على الـ
+
+73
+00:04:48,390 --> 00:04:51,590
+Light Compound Microscope الـ Light Compound
+
+74
+00:04:51,590 --> 00:04:54,270
+Microscope ليش أنا سميته بهذا الإسم الـ Light لأن
+
+75
+00:04:54,270 --> 00:04:58,520
+الضوء بيمر خلال الجسم المرات كبيرهcompound لأنه
+
+76
+00:04:58,520 --> 00:05:02,220
+بيتركب من عدستان عدسة عينية الـ ocular lens وعدسة
+
+77
+00:05:02,220 --> 00:05:06,020
+شيئية الـ objective lens and will compare the
+
+78
+00:05:06,020 --> 00:05:09,800
+relative size and shape of various microorganisms
+
+79
+00:05:09,800 --> 00:05:13,740
+من خلاله أنا بتعرف على الحجم النسبي للبكتيريا
+
+80
+00:05:13,740 --> 00:05:20,340
+وأيضا أشكال الكائنات الدقيقة المختلفة microscope
+
+81
+00:05:20,340 --> 00:05:23,680
+consists of four major parts بقولك المايكروسكوب
+
+82
+00:05:23,680 --> 00:05:28,470
+بتكون من أربع مكونات أساسيةأول مكوّن عندنا اللي هو
+
+83
+00:05:28,470 --> 00:05:31,950
+الـ Framework الـ Framework اللي هو إطار العمل
+
+84
+00:05:31,950 --> 00:05:36,010
+إطار العمل بتكون من تلت أجزاء Arm, Stache, Pulse
+
+85
+00:05:36,010 --> 00:05:40,350
+هنتكلم عنها بالتفصيل عندي تاني جزء من أزاق الـ
+
+86
+00:05:40,350 --> 00:05:44,310
+Microscope المهمة الـ Adjustment System نظام
+
+87
+00:05:44,310 --> 00:05:48,090
+الضابط نظام الضابط بتكون من الـ Fine Adjustment و
+
+88
+00:05:48,090 --> 00:05:51,230
+الـ Coarse Adjustment اللي هو الضابط الصغير و
+
+89
+00:05:51,230 --> 00:05:54,860
+الضابط الكبيرالتالت جزء اللي هو الـ Magnification
+
+90
+00:05:54,860 --> 00:05:57,840
+التكبير المسؤول عن التكبير في الـ Microscope اللي
+
+91
+00:05:57,840 --> 00:06:02,520
+هم العدسات الـ Ocular Lens العدسة العينية و الـ
+
+92
+00:06:02,520 --> 00:06:06,500
+Objective Lens العدسات الشيئية آخر جزء اللي هو الـ
+
+93
+00:06:06,500 --> 00:06:09,880
+Lighting System اللي هو نظام الإضاءة فيه عند الـ
+
+94
+00:06:09,880 --> 00:06:14,000
+Condenser اللي هو المكثف اللي بيجمع الضوء و إداف
+
+95
+00:06:14,000 --> 00:06:17,680
+راجم اللي هو بتحكم في كمية الضوء المار عبر العين
+
+96
+00:06:24,230 --> 00:06:26,990
+في هاي الـ slide هنتكلم بالتفصيل عن أجزاء
+
+97
+00:06:26,990 --> 00:06:31,830
+الميكروسكوب ووظيفة كل جزء في عندي أول جزء الـ
+
+98
+00:06:31,830 --> 00:06:36,030
+ocular lens العدسات العينية في عندي two ocular
+
+99
+00:06:36,030 --> 00:06:40,190
+lens فدول العدسات العينية شو وظيفتهم؟ remagnify
+
+100
+00:06:40,190 --> 00:06:44,670
+the image بيعمل على إعادة تكبير الصورة طيب مين
+
+101
+00:06:44,670 --> 00:06:49,810
+اللي بيعمل magnify؟ مين المسؤول الأساسي والأول عن
+
+102
+00:06:49,810 --> 00:06:54,010
+تكبير الصورة؟ نكمل الجملةformed by the objective
+
+103
+00:06:54,010 --> 00:06:57,970
+lens اللي بيتم تكبيرها بواسطة الـ objective lens
+
+104
+00:06:57,970 --> 00:07:02,630
+يبقى الـ objective lens اللي هي العدسات الشيئية هي
+
+105
+00:07:02,630 --> 00:07:06,150
+ال primary lens that magnify the specimen هي
+
+106
+00:07:06,150 --> 00:07:09,990
+العدسة الأولية اللي بتكبرلي الصورة طبعا لأن هي
+
+107
+00:07:09,990 --> 00:07:13,910
+قريبة من العينة فأكيد هتكون هي العدسة الأولية اللي
+
+108
+00:07:13,910 --> 00:07:19,180
+بتكبر الصورةremagnify الـ ocular lens بتكبّلي
+
+109
+00:07:19,180 --> 00:07:24,340
+بعدها بتوصل للصورة للعدسة العينية و بتكبّلي إياها
+
+110
+00:07:24,340 --> 00:07:29,040
+حتى إن أنا أحصل بالنهاية على total magnification
+
+111
+00:07:29,040 --> 00:07:31,800
+إيش يعني total magnification؟ وحصل ضرب ال
+
+112
+00:07:31,800 --> 00:07:35,020
+objective magnification قوة تكبير العدسة الشيئية
+
+113
+00:07:35,020 --> 00:07:38,840
+اللي أنا استخدمتها مع قوة تكبير العدسة العينية
+
+114
+00:07:38,840 --> 00:07:42,380
+الـocular magnification اللي هي العشرة X قوة تكبير
+
+115
+00:07:42,380 --> 00:07:46,860
+العدسة العينية عشرة X لكنقوة تكبير العدسة الشيئية
+
+116
+00:07:46,860 --> 00:07:51,340
+في عيني انا اربع عدسات اربع عشرة اربعين مية فهو
+
+117
+00:07:51,340 --> 00:07:55,080
+استخدمت بدربها في قوة تكبير العدسة العينية العشرة
+
+118
+00:07:55,080 --> 00:07:59,940
+X حتى احصل على ال total magnification بالنسبة
+
+119
+00:07:59,940 --> 00:08:05,140
+للعدسات الشيئية العدسات الشيئية مربوطين باشي اسمه
+
+120
+00:08:05,140 --> 00:08:11,910
+nosepiece هذاهو الـ Nosepiece قُرص دائري أو جزء
+
+121
+00:08:11,910 --> 00:08:15,970
+دائري بيحمل العدسات الشيئية بنتحكم فيه من خلال
+
+122
+00:08:15,970 --> 00:08:20,170
+التدوير وبالتالي بيؤدي لغيير قوة التكبير اللي أنا
+
+123
+00:08:20,170 --> 00:08:23,750
+بدي إياها أربعة أو عشرة أو أربعين أو مية حسب أنا
+
+124
+00:08:23,750 --> 00:08:30,810
+شو ما بدي بالنسبة للـ Body نيجي للـ Body الـ Body
+
+125
+00:08:30,810 --> 00:08:34,310
+هو إيه اسم تاني اللي هو الـ Optical Tube الأنبوب
+
+126
+00:08:34,310 --> 00:08:38,250
+البصريtransmit the image from the objective lens
+
+127
+00:08:38,250 --> 00:08:42,670
+to the ocular lens using prism بقولك بينقل الصورة
+
+128
+00:08:42,670 --> 00:08:46,570
+من ال objective lens لال ocular lens ننتبه أنه مش
+
+129
+00:08:46,570 --> 00:08:49,950
+العكس لأنه احنا قلنا أن ال objective lens هي
+
+130
+00:08:49,950 --> 00:08:54,410
+المسئولة عن التكبير بشكل أولي لأنه هي المسئولة
+
+131
+00:08:54,410 --> 00:08:56,910
+الأولى عن التكبير فبالتالي هو بينقل من ال
+
+132
+00:08:56,910 --> 00:09:02,240
+objective لالـ Ocular Lens الـ Arm اللي هو الذراع،
+
+133
+00:09:02,240 --> 00:09:05,260
+الذراع أنا بستخدمه لحمل الـ Microscope و نقله من
+
+134
+00:09:05,260 --> 00:09:09,960
+مكان إلى آخر بالنسبة للـ Stage، الـ Stage هي عبارة
+
+135
+00:09:09,960 --> 00:09:14,000
+عن سيما أحنا شايفين سطح مستوي يوجد تحت العدسات
+
+136
+00:09:14,000 --> 00:09:17,920
+الشيئية مخصص لوضع الشريحة عليه hold the microscope
+
+137
+00:09:17,920 --> 00:09:22,100
+slide in position مخصص لوضع ال slide أو الشريحة
+
+138
+00:09:22,100 --> 00:09:27,210
+عليه فيندي أنا ملقتان clipsبساعد في تثبيت الشريحة
+
+139
+00:09:27,210 --> 00:09:31,190
+على الاستاج وضمان عدم تحركها أثناء شغله في موجود
+
+140
+00:09:31,190 --> 00:09:34,710
+تحت اللي هو الاستاج شيء اسمه الـ Condenser اللي هو
+
+141
+00:09:34,710 --> 00:09:38,890
+المكثف مش وظيفته focus light through the specimen
+
+142
+00:09:38,890 --> 00:09:44,450
+بيقولك بيجمع أو بيركز الشقة الضوء المتجهة نحو
+
+143
+00:09:44,450 --> 00:09:49,830
+الشريحة وبعدين هتمار عبر العدسات الشيئية طبعا
+
+144
+00:09:51,530 --> 00:09:54,230
+الكندنسر فيه تحته «Diaphragm» مايعني الـ
+
+145
+00:09:54,230 --> 00:09:57,270
+«Diaphragm» مسئول عن «control of the amount of
+
+146
+00:09:57,270 --> 00:10:01,290
+light entering the condenser» بتحكم بكمية الضوء
+
+147
+00:10:01,290 --> 00:10:05,930
+الواصلة للكندنسر وبعدين هتوصل للعينة ثم العدسات
+
+148
+00:10:05,930 --> 00:10:10,950
+الشيئية في عندي أكيد مصدر للضوء أخر الشيء في عندي
+
+149
+00:10:10,950 --> 00:10:16,210
+ضابطين على جانبي المشهر ضابط كبير «course focusing
+
+150
+00:10:16,210 --> 00:10:20,890
+knob» وضابط صغير «fine focusing knob»شو وظيفة الـ
+
+151
+00:10:20,890 --> 00:10:24,610
+Course Focusing Knob؟ وظيفته «Move the stage up
+
+152
+00:10:24,610 --> 00:10:27,210
+and down to focus the image» بقولك بحرك الـ
+
+153
+00:10:27,210 --> 00:10:31,330
+«stage» لفقه و لتحت حتى أنه يثبت الصورة مش يعني
+
+154
+00:10:31,330 --> 00:10:35,410
+focus the image نفس معناه أنه أنا عشان أقدر أجيب
+
+155
+00:10:35,410 --> 00:10:38,890
+ال field عشان أقدر أجيب ال field بحرك ال «stage»
+
+156
+00:10:38,890 --> 00:10:42,610
+لأعلى أو لأسفل حتى أقدر أجيبه في الصورة اللي أنا
+
+157
+00:10:42,610 --> 00:10:46,680
+بديها هيك أنا معناه اقدرت أجيب ال fieldالـ «Find
+
+158
+00:10:46,680 --> 00:10:50,240
+Focusing Knob» هو الضابط الصغير الوظيفته اللي هو
+
+159
+00:10:50,240 --> 00:10:54,000
+الـ «Clarity» الوضوح حتى إنه يزيد وضوح الصورة أنا
+
+160
+00:10:54,000 --> 00:10:58,480
+بستخدم بعد ما أجيب الفيل تمام جبت الفيل لو انتقلت
+
+161
+00:10:58,480 --> 00:11:03,180
+لعدسة «High Power» لقوة تكبير أعلى بلاقي الصورة
+
+162
+00:11:03,180 --> 00:11:07,020
+مغبشة فأنا بروح بلعب بالـ «Find» عشان إنه تزيد
+
+163
+00:11:07,020 --> 00:11:10,420
+وضوح الصورة تزيد الـ «Clarity» الـ «Base» هي
+
+164
+00:11:10,420 --> 00:11:15,040
+الركيزة الأساسية للمشهر واللي هي بتحمل الـ
+
+165
+00:11:15,040 --> 00:11:19,720
+«Microscope»هذا بالنسبة لأجزاء الـ «مايكروسكوب»
+
+166
+00:11:19,720 --> 00:11:23,960
+تعرفنا على وظيفة
+
+167
+00:11:23,960 --> 00:11:27,800
+كل جزء لكن هنتكلم بالتفصيل عن كل جزء في الـ
+
+168
+00:11:27,800 --> 00:11:29,240
+«سلايدات» اللي بعيدة
+
+169
+00:11:38,830 --> 00:11:42,710
+تكلمنا إن الميكروسكوب بتكون من أربع أزاق من ضمن
+
+170
+00:11:42,710 --> 00:11:45,370
+هذه الأجزاء أول شيء اللي هو الـ Framework الـ
+
+171
+00:11:45,370 --> 00:11:48,450
+Framework بتكون من تلت أجزاء الـ Base اللي هي
+
+172
+00:11:48,450 --> 00:11:51,850
+القاعدة و الـ Arm اللي هو الذراع من خلال اللي قلنا
+
+173
+00:11:51,850 --> 00:11:55,590
+بحمل الميكروسكوب وبنقله من مكان لآخر و الـ
+
+174
+00:11:55,590 --> 00:11:58,730
+Mechanical Stage طب أنا لو بدي أعرف شو هي الـ
+
+175
+00:11:58,730 --> 00:12:02,550
+Stage Stage is the location of the specimen to be
+
+176
+00:12:02,550 --> 00:12:07,930
+viewed أقولك هو الموقع اللي أنا بحط في الشريحة أو
+
+177
+00:12:07,930 --> 00:12:11,630
+العينةEclipse utilized in holding the specimen in
+
+178
+00:12:11,630 --> 00:12:14,330
+place هاي طبعاً هي كلها الـ stage في عندي أنا
+
+179
+00:12:14,330 --> 00:12:17,870
+clips هاي هي الـ clips هي الملاقت اللي بتستخدم
+
+180
+00:12:17,870 --> 00:12:21,290
+لتثبيت العين على الـ stage و ضمان عدم تحركها
+
+181
+00:12:21,290 --> 00:12:25,130
+mechanical stage انه هي ممكن انا احركها كيف انا
+
+182
+00:12:25,130 --> 00:12:29,390
+ممكن احركها باستخدام الـ stage knob control في
+
+183
+00:12:29,390 --> 00:12:32,730
+عندي top knob و في عندي bottom knob هذا هو ال top
+
+184
+00:12:32,730 --> 00:12:36,590
+knob وهذا ال bottom knobهدولا الـ Stage knob
+
+185
+00:12:36,590 --> 00:12:41,310
+control أنا بحرك فيهم الـ Stage لليمين و لا اليسار
+
+186
+00:12:41,310 --> 00:12:44,490
+و لا الأمام و لا الخلف طيب متى أنا بحتاج هذا
+
+187
+00:12:44,490 --> 00:12:48,810
+الاشياء على سبيل المثال أنا في عندي slide و بدي
+
+188
+00:12:48,810 --> 00:12:52,530
+أشوف كل هذه الشريحة بدي أمر على كل هذه الشريحة
+
+189
+00:12:52,530 --> 00:12:58,290
+فعشان أنا ما أضيع ولا field بروح بمشي زيجزاج بمشي
+
+190
+00:12:58,290 --> 00:13:02,150
+بهذه الطريقة حتى إن أنا أضمن إن أنا مريت على كل
+
+191
+00:13:02,150 --> 00:13:03,430
+الشريحة
+
+192
+00:13:05,580 --> 00:13:09,580
+طبعا فانا هان هستخدم ال stage knob control حتى
+
+193
+00:13:09,580 --> 00:13:13,160
+انها تساعدني في الحركة فعشان انا اول شي هبدأ
+
+194
+00:13:13,160 --> 00:13:18,640
+بالحركة للامام فعشان انا اتحرك للامام هستخدم ال
+
+195
+00:13:18,640 --> 00:13:22,060
+top knob is to move the forward and backward
+
+196
+00:13:22,060 --> 00:13:25,720
+هيحركلي ال stage للامام و للخلف انا بدي اتحرك
+
+197
+00:13:25,720 --> 00:13:28,900
+للامام هستخدم ال top knob فبدي ارجع في field لأ
+
+198
+00:13:28,900 --> 00:13:31,400
+بدي ارجع اشوفه بقى استخدم برضه ال top knob
+
+199
+00:13:31,400 --> 00:13:37,280
+بحركليها للامام و للخلفطيب بالنسبة للـ Bottom Knob
+
+200
+00:13:37,280 --> 00:13:42,800
+أنا خلصت كل هذه المنطقة بدي أروح هاجي أتحرك لليمين
+
+201
+00:13:42,800 --> 00:13:46,540
+فبستخدم الـ Bottom Knob is to move the stage right
+
+202
+00:13:46,540 --> 00:13:50,400
+and left يبقى أعرفنا ليش أنا بستخدم الـ Top Knob و
+
+203
+00:13:50,400 --> 00:13:53,720
+الـ Bottom Knob الـ Top حتى أني أنا أحرك للأمام و
+
+204
+00:13:53,720 --> 00:13:57,720
+للخلف و الـ Bottom حتى أني أحرك للـSlide لليمين و
+
+205
+00:13:57,720 --> 00:13:58,680
+للشمال
+
+206
+00:14:05,000 --> 00:14:08,180
+نجي لخلصنا من أول مكون من مكونات الـ Microscope
+
+207
+00:14:08,180 --> 00:14:11,920
+اللي هو الـ Adjustment System الـ Framework نجي
+
+208
+00:14:11,920 --> 00:14:15,120
+لتاني مكون اللي هو الـ Adjustment System الـ
+
+209
+00:14:15,120 --> 00:14:18,640
+Adjustment System هو نظام الضبطبتكون من الـ
+
+210
+00:14:18,640 --> 00:14:22,720
+Optical Tube الـ Optical Tube اللي هو اللي عرفناه
+
+211
+00:14:22,720 --> 00:14:26,080
+في الأول slide في المعمل كان الـ Body اللي هو
+
+212
+00:14:26,080 --> 00:14:32,340
+بينقل الصورة من الـ Objective Lens للـ Ocular Lens
+
+213
+00:14:32,340 --> 00:14:35,540
+يعني بيروبط ما بين العدسة العينية وما بين العدسة
+
+214
+00:14:35,540 --> 00:14:39,360
+الشيئية في عندي الcourse adjustment الضابط الكبير
+
+215
+00:14:39,360 --> 00:14:42,720
+و ال fine adjustment اللي هو الضابط الصغير هنتعرف
+
+216
+00:14:42,720 --> 00:14:46,050
+عليهم في هاي ال slideالـ Course Adjustment
+
+217
+00:14:46,050 --> 00:15:13,790
+Knob�
+
+218
+00:15:14,270 --> 00:15:18,630
+bringing the objective هي إن حتى إن أنا أجيب ال
+
+219
+00:15:18,630 --> 00:15:21,990
+field الضابط الكبير وظيفته إن أنا أقدر أجيب ال
+
+220
+00:15:21,990 --> 00:15:24,970
+field ال field هو الصورة اللي أنا بدي أشوف هيبقى
+
+221
+00:15:24,970 --> 00:15:28,530
+bringing the image أو focus the image هيوظيفة ال
+
+222
+00:15:28,530 --> 00:15:31,690
+course adjustment knob بالنسبة لأ ال fine
+
+223
+00:15:31,690 --> 00:15:35,690
+adjustment knob is used for improving the clarity
+
+224
+00:15:35,690 --> 00:15:39,610
+حتى إنه يزيد وضوح الصورة especially when viewing
+
+225
+00:15:39,610 --> 00:15:43,690
+under high powerخصوصًا لما أنا أستخدم الـHigh
+
+226
+00:15:43,690 --> 00:15:47,970
+Power مش قصده بيه هذه الجملة خلال الشغل العملي لما
+
+227
+00:15:47,970 --> 00:15:51,770
+أنا أجيب الـField على 4X و بعدين أروح أحول على 40X
+
+228
+00:15:51,770 --> 00:15:55,110
+أو على 100 اللي هي الـHigh Power بلاقي الصورة
+
+229
+00:15:55,110 --> 00:15:59,950
+مغبشة فعشان أنا أزيد وضوح الصورة يتكون improving
+
+230
+00:15:59,950 --> 00:16:02,850
+the clarity of the image بروح بحرك الـFine
+
+231
+00:16:02,850 --> 00:16:06,930
+Adjustment شوية فبالتالي هيك بتكون الصورة واضحة
+
+232
+00:16:06,930 --> 00:16:09,550
+وفي بعض الأمور التانية اللي ممكن كمان إن أنا أتحكم
+
+233
+00:16:09,550 --> 00:16:14,770
+فيها نتكلم عنهافي الـ «سلايدات» اللي جاية فأنا هان
+
+234
+00:16:14,770 --> 00:16:17,390
+بأستخدم فقط الـ «fine adjustment» جبت الـ «field»
+
+235
+00:16:17,390 --> 00:16:21,110
+ممنوع أستخدم الـ «course adjustment» خلاص فقط بحرك
+
+236
+00:16:21,110 --> 00:16:25,410
+الـ «fine adjustment» عشان تزيد وضوح الصورة ممنوع
+
+237
+00:16:25,410 --> 00:16:28,750
+أمسك الـ «course adjustment» هذا بالنسبة لـ
+
+238
+00:16:28,750 --> 00:16:32,770
+«course» والـ «fine adjustment knob» هاي مكونات
+
+239
+00:16:32,770 --> 00:16:37,290
+الـ «adjustment system» نيجي لآخر مكون اللي هو الـ
+
+240
+00:16:37,290 --> 00:16:39,930
+«interbiolary adjustment»
+
+241
+00:16:41,930 --> 00:16:45,210
+بشهدها الـ Inter-bipolary adjustment ‏to control
+
+242
+00:16:45,210 --> 00:16:48,230
+the distance ‏between the ocular lens ‏to adopt
+
+243
+00:16:48,230 --> 00:16:52,910
+the distance between viewer eye ‏قولنا إن المسافة
+
+244
+00:16:52,910 --> 00:16:58,750
+بين عينان أي شخص بتختلف ‏فبالتالي عشان أتحكم في
+
+245
+00:16:58,750 --> 00:17:03,170
+المسافة ‏ما بين العدسات العينية ‏حتى إنها تكون
+
+246
+00:17:03,170 --> 00:17:07,610
+مناسبة ‏لمسافة ما بين عينان الرأي ‏هذه أنا بستخدم
+
+247
+00:17:07,610 --> 00:17:12,950
+الـ Inter-bipolary adjustmentالـ Eye-Base Lens هي
+
+248
+00:17:12,950 --> 00:17:17,430
+نفسها الـ Ocular Lens ممكن هو الـ Spread apart
+
+249
+00:17:17,430 --> 00:17:22,290
+ممكن أبعدهم عن بعض أو Get closer together to fit
+
+250
+00:17:22,290 --> 00:17:28,130
+each individual أو ممكن أقربهم حسب شخص بيرتاح حسب
+
+251
+00:17:28,130 --> 00:17:32,690
+ما بيرتاح ممكن أن أنا أبعدهم أو أقربهم هذا بالنسبة
+
+252
+00:17:32,690 --> 00:17:34,410
+للـ Adjustment
+
+253
+00:17:42,630 --> 00:17:45,930
+ثالث مكوّن من مكونات الميكروسكوب الـ Magnification
+
+254
+00:17:45,930 --> 00:17:48,590
+قلنا المسؤول عن الـ Magnification العدسات الـ
+
+255
+00:17:48,590 --> 00:17:51,530
+Ocular Lens العدسات العينية الـ IBIS اللي هي اسم
+
+256
+00:17:51,530 --> 00:17:54,910
+تاني و الـ Objective Lens اللي هي العدسات الشيئية
+
+257
+00:17:54,910 --> 00:17:59,510
+الـ Ocular Lens or الـ IBIS is used for viewing
+
+258
+00:17:59,510 --> 00:18:04,510
+العدسات العينية تستخدم للرؤية Revolving Nose Views
+
+259
+00:18:04,510 --> 00:18:07,810
+contain Objective Lens that are used to magnify
+
+260
+00:18:07,810 --> 00:18:12,270
+قلنا إن في أندي أنا هان إيش اسمهNosepiece هذا هو
+
+261
+00:18:12,270 --> 00:18:16,770
+الـ Nosepiece قرص دائري من خلاله أنا بدور العدسات
+
+262
+00:18:16,770 --> 00:18:20,770
+الشيئية حسب أنا شو بدي بحرك و بختار العدسة الشيئية
+
+263
+00:18:20,770 --> 00:18:25,210
+اللي أنا بدي استخدمها بقولك هذا اسمه في العربية
+
+264
+00:18:25,210 --> 00:18:28,650
+فيه اسم اللي هو الطاحونة بيحتوى بيحمل العدسات
+
+265
+00:18:28,650 --> 00:18:33,220
+الشيئية شو وظيفة العدسات؟تستخدم لـ Magnify لتكبير
+
+266
+00:18:33,220 --> 00:18:36,880
+الصورة بالاتحاد مع الـ Ocular Lens واتفقنا إنه
+
+267
+00:18:36,880 --> 00:18:39,560
+بالنهاية أنا بحصل على Total Magnification من حصل
+
+268
+00:18:39,560 --> 00:18:41,980
+ضرب الـ Magnification للعدسات العينية في الـ
+
+269
+00:18:41,980 --> 00:18:45,720
+Magnification للعدسات الشائعية The objective of
+
+270
+00:18:45,720 --> 00:18:48,760
+microscope is not just to increase magnification
+
+271
+00:18:48,760 --> 00:18:53,440
+but to do so while attaining sufficient resolution
+
+272
+00:18:54,450 --> 00:18:57,230
+في هذه الجملة في أول معمل الـ Microscope قلنا إن
+
+273
+00:18:57,230 --> 00:19:01,150
+الـ Microscope هو تقنية بيجعل الأشياء الصغيرة يمكن
+
+274
+00:19:01,150 --> 00:19:05,070
+رؤيتها بالعين المجردة من خلال تكبيرها بالجملة هذه
+
+275
+00:19:05,070 --> 00:19:08,690
+بقول إن أنا مش هدفي من الـ Microscope فقط إن أنا
+
+276
+00:19:08,690 --> 00:19:12,750
+أكبر الأشياء كمان لازم أحصل على دقة عالية للصورة
+
+277
+00:19:12,750 --> 00:19:16,830
+تكون واضحة على سبيل المثال لو وقت كتير بنزل صورة
+
+278
+00:19:16,830 --> 00:19:20,090
+على الجوال بروح أنا بعمل zoom بكبر عشان أشوف بعض
+
+279
+00:19:20,090 --> 00:19:25,980
+التفاصيل ما بقدر أشوفها بشكل واضحفبالتالي هان ممكن
+
+280
+00:19:25,980 --> 00:19:30,640
+تكون الصورة مكبرة ولكن مش واضحة نطبق الموضوع على
+
+281
+00:19:30,640 --> 00:19:33,980
+الـ Microscope لما أنت تجيبي الـ Field على 10X و
+
+282
+00:19:33,980 --> 00:19:38,180
+تروحي على 40X الـ High Power هتلاحظي أن الصورة
+
+283
+00:19:38,180 --> 00:19:41,780
+مغبشة و مش واضحة عشان تحصلي على Resolution عالي
+
+284
+00:19:41,780 --> 00:19:45,580
+على دقة عالية بتروحي بتحركي الـ Point Adjustment
+
+285
+00:19:45,580 --> 00:19:50,710
+اللي هو الضابط الصغير حتى تحصلي على دقة عاليةوكمان
+
+286
+00:19:50,710 --> 00:19:54,790
+ممكن أنه نتحكم في أمور تانية هنتكلم عنها في الـ
+
+287
+00:19:54,790 --> 00:19:58,770
+Slides اللي جاي يبقى مش بس الهدف من الـ Microscope
+
+288
+00:19:58,770 --> 00:20:02,270
+أني أنا أكبر لازم أني أنا أحصل على دقة عالية من
+
+289
+00:20:02,270 --> 00:20:04,210
+خلال التحكم في بعض في الأمور
+
diff --git a/PL9fwy3NUQKwZWgAb_V1ATlRRsH-6i3cPC/XkWBgngbjy4_postprocess.srt b/PL9fwy3NUQKwZWgAb_V1ATlRRsH-6i3cPC/XkWBgngbjy4_postprocess.srt
new file mode 100644
index 0000000000000000000000000000000000000000..3f3b9aa2ddc40394983563ec53aba2a27683a7dc
--- /dev/null
+++ b/PL9fwy3NUQKwZWgAb_V1ATlRRsH-6i3cPC/XkWBgngbjy4_postprocess.srt
@@ -0,0 +1,1476 @@
+1
+00:00:00,690 --> 00:00:03,830
+تاني نوع من أنواع الـ special stain الـ capsular
+
+2
+00:00:03,830 --> 00:00:06,490
+stain قلنا إنه الـ capsule أحد الـ special
+
+3
+00:00:06,490 --> 00:00:09,770
+component اللي عدم وجودها لا يؤثر على الخلية
+
+4
+00:00:09,770 --> 00:00:13,190
+البكتيرية يعني في عندي بكتيريا capsulated وفي
+
+5
+00:00:13,190 --> 00:00:17,470
+بكتيريا non-capsulated capsule are gelatinous
+
+6
+00:00:17,470 --> 00:00:22,010
+outer layer structure هو طبقة خارجية secreted by
+
+7
+00:00:22,010 --> 00:00:25,730
+bacterial cells that lay outside of an organism
+
+8
+00:00:25,730 --> 00:00:30,060
+cell wallبكون خارج الخلية البكتيرية and these are
+
+9
+00:00:30,060 --> 00:00:32,900
+in direct contact with the environment فبالتالي
+
+10
+00:00:32,900 --> 00:00:36,680
+هيكون ملامس للبيئة الخارجية الـ capsule بيتم
+
+11
+00:00:36,680 --> 00:00:40,540
+تصنيعه فيه الـ cytoplasm and secret to the outside
+
+12
+00:00:40,540 --> 00:00:45,240
+of the cell وبيطلع خارج الخلية البكتيرية where it
+
+13
+00:00:45,240 --> 00:00:49,340
+surrounds the bacteria وبحيط في هذه الخليةmany
+
+14
+00:00:49,340 --> 00:00:53,700
+perhaps most bacteria produce capsule under the
+
+15
+00:00:53,700 --> 00:00:56,860
+right condition بيقوللي معظم البكتيريا بتكون
+
+16
+00:00:56,860 --> 00:01:00,320
+الكبسول في ال right condition إيش يعني في ال right
+
+17
+00:01:00,320 --> 00:01:03,120
+condition؟ يعني لو كانت الظروف مناسبة أو كان في
+
+18
+00:01:03,120 --> 00:01:08,860
+عندي مغذيات المغذيات هي الفائض من المغذيات بتتخزن
+
+19
+00:01:08,860 --> 00:01:13,040
+في الكبسول من وظائف الكبسول إنه يعتبر مخزن للمواد
+
+20
+00:01:13,040 --> 00:01:16,100
+الغذائية في حال كان في عندي فائض
+
+21
+00:01:19,770 --> 00:01:25,650
+Capsules are fragile بقولّي إن الكبسول هش and can
+
+22
+00:01:25,650 --> 00:01:30,370
+be dimensioned dissociated, destroyed or destroyed
+
+23
+00:01:30,370 --> 00:01:34,550
+by heat بقولّي إن الكبسول if fragile فبالتالي
+
+24
+00:01:34,550 --> 00:01:40,310
+هيتقلص و هيصير جاف و هيتشوّه و يتدمر بواسطة
+
+25
+00:01:40,310 --> 00:01:44,950
+الحرارة من خطوات لستان قلنا إن في عندي أول إشسمير
+
+26
+00:01:44,950 --> 00:01:49,540
+preparation air drying heat fixationبما أن
+
+27
+00:01:49,540 --> 00:01:53,660
+الكابسول إفراجيل وبتكسر بواسطة الحرارة يبقى خطوة
+
+28
+00:01:53,660 --> 00:01:58,360
+الـ heat fixation في الكابسولارستان مش هعملها لأنه
+
+29
+00:01:58,360 --> 00:02:02,400
+هيدمر الكابسول من خلال هذه الخطوة لو أنا عملتها
+
+30
+00:02:02,400 --> 00:02:08,060
+فبالتالي هيعطيني false result most capsule are
+
+31
+00:02:08,060 --> 00:02:13,440
+composed of polysaccharide بقولي معظم البكتيريا
+
+32
+00:02:13,440 --> 00:02:16,880
+اللي بتكون كابسول هي كابسول عبارة عن
+
+33
+00:02:16,880 --> 00:02:21,370
+polysaccharidebut some are composed of polypeptide
+
+34
+00:02:21,370 --> 00:02:25,750
+لكن في بعض البكتيريا الكابسول تبعها polypeptide زي
+
+35
+00:02:25,750 --> 00:02:31,170
+البصيلاس أنتراكسي بيوكايد كولوني showing viscous
+
+36
+00:02:31,170 --> 00:02:35,110
+or sticky growth typical of an organism producing
+
+37
+00:02:35,110 --> 00:02:39,090
+large quantities of a carbohydrate capsule قولنا
+
+38
+00:02:39,090 --> 00:02:43,470
+معظم البكتيريا اللي بتكون كابسول الكولوني تبعها
+
+39
+00:02:43,470 --> 00:02:46,210
+على الطبق في ان احنا كولوني morphology ممكن من
+
+40
+00:02:46,210 --> 00:02:51,210
+خلاله اتعرف على البكتيرياالمصدع مرات على الطبق بعد
+
+41
+00:02:51,210 --> 00:02:54,430
+ما أنا أنمي هذه البكتيريا بتكون طبيعتها Viscous
+
+42
+00:02:54,430 --> 00:02:57,830
+بتكون لذجة على سبيل المثال الكليبسيلة تعتبر
+
+43
+00:02:57,830 --> 00:03:02,510
+capsulated لما تيجي تاخدي كولوني كليبسيلة باللوب
+
+44
+00:03:02,510 --> 00:03:06,410
+بتلاقيها بتمغط هذا نتيجة الكميات الكبيرة من
+
+45
+00:03:06,410 --> 00:03:09,970
+الكربوهيدرات اللي بتكون منها الكابسول قولنا ان
+
+46
+00:03:09,970 --> 00:03:12,330
+الكابسول polysaccharide يعني carbohydrate
+
+47
+00:03:15,880 --> 00:03:20,620
+Capsules function شو وظائف الكبسول أول وظيفة
+
+48
+00:03:20,620 --> 00:03:24,740
+protect the cell from drying هيحميل البكتيريا من
+
+49
+00:03:24,740 --> 00:03:29,180
+الجفاف الكبسول هيعمل inhibit لالwater from
+
+50
+00:03:29,180 --> 00:03:33,160
+escaping into environment فبالتالي هيحمي الخلية
+
+51
+00:03:33,160 --> 00:03:37,280
+البكتيرية من الجفاف protect the cell from
+
+52
+00:03:37,280 --> 00:03:42,070
+phagocyte being engulfed by white blood cellبيحمي
+
+53
+00:03:42,070 --> 00:03:46,890
+الخلية من الـ phagocytosis لأن الكبسول لذج فسكس
+
+54
+00:03:46,890 --> 00:03:50,490
+فلما تيجي الـ phagocyte اللي هي الـ White BCs عشان
+
+55
+00:03:50,490 --> 00:03:55,590
+تعمل Engulfment تلتهم الخلية بتروح الخلية بتهرب
+
+56
+00:03:55,590 --> 00:04:01,050
+لأن الخلية بتحتوي على الكبسول اللذج فتهرب من جهاز
+
+57
+00:04:01,050 --> 00:04:04,510
+المناعة فتهرب من الـ White BCs وهذا يعتبر
+
+58
+00:04:04,510 --> 00:04:09,460
+Virulence Factor عامل من عوامل الشرسةتالت وظيفة
+
+59
+00:04:09,460 --> 00:04:12,840
+provide a food reserve when a certain organic
+
+60
+00:04:12,840 --> 00:04:16,980
+compounds are in excess بيقول يعتبر مخزن للمواد
+
+61
+00:04:16,980 --> 00:04:21,700
+الغذائية في حال عندي كان فائض من هذه المواد هتتخزن
+
+62
+00:04:21,700 --> 00:04:26,460
+في الكابسول رابع وظيفة يعتبر قولنا ان هو virulence
+
+63
+00:04:26,460 --> 00:04:30,040
+factor عامل من عوامل الشرازة of pathogenic
+
+64
+00:04:30,040 --> 00:04:35,760
+microbes خامس وظيفةthey serve as binding or
+
+65
+00:04:35,760 --> 00:04:39,380
+adhesion agent for sticking cell together or to
+
+66
+00:04:39,380 --> 00:04:44,200
+surface وقوللي الوظيفة الخامسة هي ال attachment ال
+
+67
+00:04:44,200 --> 00:04:47,800
+attachment ممكن تلتثق الخلايا البكتيرية مع بعض
+
+68
+00:04:47,800 --> 00:04:52,440
+together or to the surface أو على السطح الكابسول
+
+69
+00:04:52,440 --> 00:04:56,740
+لذج فبالتالي هتلذق البكتيريا على بعض مع بعض أو على
+
+70
+00:04:56,740 --> 00:05:01,100
+السطحطيب شوف فايدة ال attachment بقول عشان يصير في
+
+71
+00:05:01,100 --> 00:05:05,840
+عندي infection عشان يصير المرض أول خطوة لازم يصير
+
+72
+00:05:05,840 --> 00:05:10,120
+ال attachment تلتثق البكتيريا مع بعض أو تلتثق على
+
+73
+00:05:10,120 --> 00:05:14,420
+السطح بعدين هيصير في عندي colonization بعدين هيصير
+
+74
+00:05:14,420 --> 00:05:19,590
+ال infectionممكن قلنا يكون على الأسطح زي السخور أو
+
+75
+00:05:19,590 --> 00:05:23,090
+على سطح الأسنان في عندي بكتيريا الـ Streptococcus
+
+76
+00:05:23,090 --> 00:05:27,350
+mutans هذه البكتيريا بتعمل تسوص الأسنان لأنه
+
+77
+00:05:27,350 --> 00:05:35,330
+موجودة في الفم في وجود السكراسة تنتج الكابسول هذه
+
+78
+00:05:35,330 --> 00:05:38,810
+ال slide هنتكلم عن عوامل لازم أنا أخدها بعين
+
+79
+00:05:38,810 --> 00:05:43,940
+الاعتبار لما بدي أصبغ الكابسولأول معلومة بكتيريا
+
+80
+00:05:43,940 --> 00:05:49,180
+الكابسول are non-ionic قولي ان الكابسول مايله شحنة
+
+81
+00:05:49,180 --> 00:05:54,380
+so neither acidic nor basic stain will adhere to
+
+82
+00:05:54,380 --> 00:05:57,800
+their surface عشان هيك لا ال acidic stain ولا ال
+
+83
+00:05:57,800 --> 00:06:03,500
+basic stain هتقدر تلتثق بالكابسول هتبع هعمل طريقة
+
+84
+00:06:03,500 --> 00:06:06,840
+عشان اقدر اني انا اصبغ الكابسول هنتعرف عليها
+
+85
+00:06:06,840 --> 00:06:11,240
+because most capsule material are water soluble
+
+86
+00:06:11,640 --> 00:06:15,320
+simple stain will not adhere to them بقول لي معظم
+
+87
+00:06:15,320 --> 00:06:18,980
+مكونات الكابسول هي water soluble عشان هيك ال
+
+88
+00:06:18,980 --> 00:06:23,980
+simple stain مابتقدر تلتصق بالكابسول ال simple
+
+89
+00:06:23,980 --> 00:06:28,820
+stain أنا خلال ال simple stain بعمل washing بين كل
+
+90
+00:06:28,820 --> 00:06:33,040
+خطوة والتانية فبالتالي الكابسول material هيتم
+
+91
+00:06:33,040 --> 00:06:36,700
+إزالته خلال ال washing يبقى خلال شغل في الكابسول
+
+92
+00:06:36,700 --> 00:06:41,120
+stainبدي اخد بيعني الاعتبار rinsing the slide with
+
+93
+00:06:41,120 --> 00:06:45,000
+water لازم اني انا اتجنبه اني اغسل ال slide بالميه
+
+94
+00:06:45,000 --> 00:06:50,000
+لان الكابسول هيصله ازالة dislodge الكابسول هيتم
+
+95
+00:06:50,000 --> 00:06:57,700
+ازالته خلال عملية ال washing تالت معلومة ال older
+
+96
+00:06:57,700 --> 00:07:01,780
+culture are most likely to exhibit capsule
+
+97
+00:07:01,780 --> 00:07:06,490
+production بقوللي انه لما بدي انا اعملكابسول استان
+
+98
+00:07:06,490 --> 00:07:10,310
+باخد الـ colony من all their culture ليش؟ لأن الـ
+
+99
+00:07:10,310 --> 00:07:13,310
+all .. لأن في الـ all their culture بتكون المواد
+
+100
+00:07:13,310 --> 00:07:18,010
+الغذائية بدأت تتخلص و الظروف بتصبح سيئة فبالتالي
+
+101
+00:07:18,010 --> 00:07:22,730
+هيتكون عندي الكابسول نفس الاشي بالنسبة للـ Spar
+
+102
+00:07:22,730 --> 00:07:27,130
+يفضل أنها تكون all their culture لأن بتتكون
+
+103
+00:07:27,130 --> 00:07:32,070
+الكابسول و الـ Spar في .. لما تتصير الظروف البيئية
+
+104
+00:07:32,070 --> 00:07:37,040
+صعبةWhen performing a capsule stand on your
+
+105
+00:07:37,040 --> 00:07:40,740
+unknown, be sure the culture you take your sample
+
+106
+00:07:40,740 --> 00:07:44,980
+from is at least five days old.أقولك عشان هيك لما
+
+107
+00:07:44,980 --> 00:07:50,040
+بدك أنتي تعملي أي capsule stand، تاخدي الخلوني من
+
+108
+00:07:50,040 --> 00:07:56,810
+culture، من مزرعة على الأقل يكون لها خمس أيامأتراب
+
+109
+00:07:56,810 --> 00:08:01,590
+من السيرام يمكن استخدامه في تحريك لتحسين صميم
+
+110
+00:08:01,590 --> 00:08:07,750
+الكبسول وتجعله أسهل للتلاعب مع كمباون عادل مثل
+
+111
+00:08:07,750 --> 00:08:14,420
+ميكروسكوب ضوء وقلي أثناء عمليلأ لسمير في الكابسول
+
+112
+00:08:14,420 --> 00:08:19,100
+الستان ممكن اضيف drop of serum drop of serum بقول
+
+113
+00:08:19,100 --> 00:08:23,760
+هذا هيعمل على زيادة حجم الكابسول enhance the size
+
+114
+00:08:23,760 --> 00:08:26,480
+of the capsule يبقى هي وضيفت اللي هو ال serum
+
+115
+00:08:26,480 --> 00:08:31,700
+فبالتالي انا هقدر اشوف الكابسول بشكل واضح وقت اش
+
+116
+00:08:31,700 --> 00:08:35,500
+بحط اللي هو drop of serum عندنا هنتعرف خلال السمير
+
+117
+00:08:35,500 --> 00:08:40,920
+انا بضيف كولوني و هضيف معه اللي هو drop of serum
+
+118
+00:08:44,070 --> 00:08:47,990
+كيف بدي انا اصبغ الكابسول في عندي انا طريقتين
+
+119
+00:08:47,990 --> 00:08:51,670
+لصباغة الكابسول ال acid fast اذا بتتذكروا باصل
+
+120
+00:08:51,670 --> 00:08:56,910
+لايكان في عندي تلك طرق لصباغتها بدي انا اكون عارفة
+
+121
+00:08:56,910 --> 00:08:59,990
+كل الطرق و شو ال principle لكل طريقة و كيف بتكون
+
+122
+00:08:59,990 --> 00:09:04,550
+نتيجة كل منها بالنسبة لالكابسول أستان اول طريقة
+
+123
+00:09:04,550 --> 00:09:10,390
+اسمها الgens stand method بشكل مختصر احنا قلنا انه
+
+124
+00:09:10,390 --> 00:09:14,930
+الكابسول non-ionic مايله شحنةطب انا كيف بدي أصبغ
+
+125
+00:09:14,930 --> 00:09:19,270
+هذا الـ capsule بقولنا في هذه الطريقة بستخدم نوعين
+
+126
+00:09:19,270 --> 00:09:24,050
+من الصبغار بستخدم basic stain و بستخدم acidic
+
+127
+00:09:24,050 --> 00:09:28,650
+stain ال basic stain هتصبغ البكتيريا بستخدم basic
+
+128
+00:09:28,650 --> 00:09:32,570
+stain زي ال methylene blue أو ال crystal violet ال
+
+129
+00:09:32,570 --> 00:09:35,610
+acidic stain هتصبغ قولنا بتتذكروا في معمل ال
+
+130
+00:09:35,610 --> 00:09:37,730
+symbolism بتصبغ ال background
+
+131
+00:09:40,530 --> 00:09:43,830
+من الأمثلة على الـ Acidic Stain اللي ممكن استخدمها
+
+132
+00:09:43,830 --> 00:09:48,410
+زي النكروسين أو الانتياينك يبقى ال basic stain
+
+133
+00:09:48,410 --> 00:09:53,350
+صبغت البكتيريا ال acidic stain هتصبغها الخلفية ال
+
+134
+00:09:53,350 --> 00:09:57,630
+background بالتالي هيبين الكابسول على شكل hello
+
+135
+00:09:57,630 --> 00:10:02,670
+area على شكل فجوات حول البكتيريا هيقدرت أعرف هل هي
+
+136
+00:10:02,670 --> 00:10:06,650
+البكتيريا capsulated أو non capsulated بالرغم من
+
+137
+00:10:06,650 --> 00:10:11,710
+أن الكابسول ما له شحنة وما بقدر أصبغهعشان هيك بسمي
+
+138
+00:10:11,710 --> 00:10:16,650
+الـ Capsular Stain بسميها Negative Stain إيش يعني
+
+139
+00:10:16,650 --> 00:10:21,590
+Negative Stain؟ يعني مش هتقدر تاخد الصبغة لأنه
+
+140
+00:10:21,590 --> 00:10:25,450
+مايلها شحنة مش هتقدر تاخد الصبغة سواء كانت Acidic
+
+141
+00:10:25,450 --> 00:10:30,230
+أو Basic Stain مش عشان الكابسولة بيحمل شحنة سالبة
+
+142
+00:10:30,230 --> 00:10:33,070
+روحت سماتها Negative Stain لأ Negative Stain يعني
+
+143
+00:10:33,070 --> 00:10:40,040
+ماتقدر تاخد الصبغة لأنه مايلها شحنةوبهذا السؤال
+
+144
+00:10:40,040 --> 00:10:44,480
+مهم جدا ليش أنا بسمي ال capsular stain ب negative
+
+145
+00:10:44,480 --> 00:10:48,760
+stain نقرا
+
+146
+00:10:48,760 --> 00:10:52,620
+ال slide جينز method هانكتبلي ال principle تبعت
+
+147
+00:10:52,620 --> 00:10:56,660
+هاي الطريقة in this stain we used acidic and basic
+
+148
+00:10:56,660 --> 00:11:01,700
+dye بستخدم acidic و basic dye acidic dye as india
+
+149
+00:11:01,700 --> 00:11:04,740
+ink and negrosin بقولي من الأمثلة على ال acidic
+
+150
+00:11:04,740 --> 00:11:09,000
+dye ال india ink و ال negrosinUse to stain
+
+151
+00:11:09,000 --> 00:11:12,440
+background of the slide بقولي بتستخدم لصباغة ال
+
+152
+00:11:12,440 --> 00:11:17,180
+background and but basic stain ال basic stain زي
+
+153
+00:11:17,180 --> 00:11:20,080
+ال methylene blue أو ال crystal violet هتصبغ
+
+154
+00:11:20,080 --> 00:11:25,860
+الخلية البكتيرية بقولي ال India ink هتعطي semi
+
+155
+00:11:25,860 --> 00:11:30,180
+opaque background هتعطي الخلفية إشي معتم بالمقارنة
+
+156
+00:11:30,180 --> 00:11:34,620
+إنه الكابسول هيك هيبين هيكون واضح وهقدر أني أنا
+
+157
+00:11:34,620 --> 00:11:37,360
+أشوفه بالرغم من إنه ما له شحنة
+
+158
+00:11:42,080 --> 00:11:46,580
+بالنسبة لخطوات الشغل اول اش هعمل smear preparation
+
+159
+00:11:46,580 --> 00:11:50,720
+طريقة الـsmear preparation في الـgens method
+
+160
+00:11:50,720 --> 00:11:55,100
+هتختلف عن اي smear preparation تانية بقوللي بجيب
+
+161
+00:11:55,100 --> 00:11:59,960
+slide بحط على طرف ال slide مش بالنص على الطرف ال
+
+162
+00:11:59,960 --> 00:12:03,280
+slide drop of acidic acid
+
+163
+00:12:08,580 --> 00:12:13,300
+الـ Drop of India Ink أو ممكن نحكي بشكل عام اللي
+
+164
+00:12:13,300 --> 00:12:27,240
+هي أسادكستان أسادكستان
+
+165
+00:12:27,240 --> 00:12:31,560
+سواء India Ink أو نيجروزين بحط drop من هذه الصبغة
+
+166
+00:12:31,560 --> 00:12:36,780
+على one end of the slide على طرف ال slide بروح
+
+167
+00:12:36,780 --> 00:12:41,260
+بجيباللي هو Loop full of bacteria البكتيريا اللي
+
+168
+00:12:41,260 --> 00:12:46,280
+بدي أشوف هل هي فيها كابسول أو لأ وبعمل mix منيح
+
+169
+00:12:46,280 --> 00:12:50,140
+بين ال drop high وما بين البكتيريا لكن بدون ما
+
+170
+00:12:50,140 --> 00:12:53,760
+أعمل توزيع لل drop في نفس المكان بعمل mix
+
+171
+00:13:01,510 --> 00:13:06,310
+بعدها بروح بعد ما اعمل مكسة منيح بجيب slide تاني و
+
+172
+00:13:06,310 --> 00:13:09,550
+زي ال blood smear زي ما كنتوا بتعمله في ال blood
+
+173
+00:13:09,550 --> 00:13:13,610
+smear كنتوا تحطوا drop من ال blood و تفرده هى نفس
+
+174
+00:13:13,610 --> 00:13:17,530
+الشيء لكن هى مش drop of blood drop من الصبغة و من
+
+175
+00:13:17,530 --> 00:13:20,350
+البكتيريا انا عاملها ال home mix بروح بجيب slide
+
+176
+00:13:20,350 --> 00:13:27,140
+على الطرفوبستنى طبعا لما ال drop تيجى على حفة ال
+
+177
+00:13:27,140 --> 00:13:32,120
+slide وبعدين برجع لورا بعدين بعمل smear ما بعرف
+
+178
+00:13:32,120 --> 00:13:38,080
+اذا في عندي صورة هاي
+
+179
+00:13:38,080 --> 00:13:42,400
+الصورة اول اشي بجيب اللى هو drop من ال acidic
+
+180
+00:13:42,400 --> 00:13:46,320
+stand سواء كانت نيجروزين انديا انج او هانذكرلى ال
+
+181
+00:13:46,320 --> 00:13:50,060
+Congo red stand بجيب ال organism اللى انا بدي
+
+182
+00:13:50,380 --> 00:13:54,160
+أفحصله البكتيريا عنده هل هي capsulated أو non
+
+183
+00:13:54,160 --> 00:13:59,620
+-capsulated باجي بـ slide تانية تكون نظيفة و باجي
+
+184
+00:13:59,620 --> 00:14:05,040
+على بالنص بعدها برجع لورا باستنى لل drop انها تيجي
+
+185
+00:14:05,040 --> 00:14:10,600
+على العلى الشريحة على طرف الشريحة الاقيها انها
+
+186
+00:14:10,600 --> 00:14:16,820
+اتوزعت بعدين بقدم لقدام عشان اعمل blood smear عشان
+
+187
+00:14:16,820 --> 00:14:20,720
+اعمل فيلم من اللي هو طبعا هذا فيلم من ال blood لكن
+
+188
+00:14:20,720 --> 00:14:26,320
+هان انا بعمل اللي هو smear ل ال acidic stain مع
+
+189
+00:14:26,320 --> 00:14:30,620
+البكتيريا هلاحظ ان المنطقة القريبة اللي كانت من ال
+
+190
+00:14:30,620 --> 00:14:35,660
+drop هتكون thick وهي المنطقة هتكون thin يبقى هاي
+
+191
+00:14:35,660 --> 00:14:36,940
+أول خطوة نرجع
+
+192
+00:14:39,730 --> 00:14:43,910
+خلصنا من أول خطوة اللى هى الـ Smear preparation
+
+193
+00:14:43,910 --> 00:14:48,150
+الـ Smear preparation قلنا اضرب من الاسدكستان مع
+
+194
+00:14:48,150 --> 00:14:53,450
+البكتيريا وبعمللهم سمير فرد زى طريقة ال blood
+
+195
+00:14:53,450 --> 00:14:57,930
+smearهذه الطريقة انا لما اروح اجيب الاسدكستان و
+
+196
+00:14:57,930 --> 00:15:02,170
+اعملها mix و افرض بزي ال blood سمير بضمن ان ال
+
+197
+00:15:02,170 --> 00:15:06,310
+background كلها انسبغط لان ممكن لو فردت بال loop
+
+198
+00:15:06,310 --> 00:15:10,030
+زي ما كنا نفرض بالسمير العادى ممكن ما اتصل
+
+199
+00:15:10,030 --> 00:15:14,710
+الاسدكستان لكل البكتيريا فبالتالي مش هقدر اشوف ال
+
+200
+00:15:14,710 --> 00:15:18,750
+capsule الخطوة التانية بعد السمير preparation هعمل
+
+201
+00:15:18,750 --> 00:15:23,980
+air dryingهل هعمل heat fixation بقولنا انه ممنوع
+
+202
+00:15:23,980 --> 00:15:28,060
+انا اعمل heat fixation السبب الأول لأنه ال capsule
+
+203
+00:15:28,060 --> 00:15:32,580
+if fragile فبالتالي ممكن يتكسر مع الحرارة ويعطيني
+
+204
+00:15:32,580 --> 00:15:37,500
+false result في عندي سبب تاني هنذكره لما قمر معانا
+
+205
+00:15:37,500 --> 00:15:47,560
+خلال ال slide بعد ما اعملالخطوة التانية هي أصبغ
+
+206
+00:15:47,560 --> 00:15:53,460
+بـ Basic Stain
+
+207
+00:15:53,460 --> 00:15:56,120
+بعد الـ air-drying
+
+208
+00:16:10,530 --> 00:16:14,650
+هنا ذاكر مثليني بلو ممكن اكريستال فيوليت بضيف
+
+209
+00:16:14,650 --> 00:16:19,110
+المثليني بلو على الاسمير بعد ما عملتله air drying
+
+210
+00:16:19,110 --> 00:16:23,970
+لمدة من اتنين لتلاتة minute من اتنين لتلت دقائق
+
+211
+00:16:23,970 --> 00:16:28,250
+بخليها في المثليني بلو بعد ما يخلصوا التلت دقائق
+
+212
+00:16:28,250 --> 00:16:31,890
+بقول لي rinse gently with water and allow to air
+
+213
+00:16:31,890 --> 00:16:36,410
+dryبعدها بغسل بالميه لكن بنحاول انه يكون gently
+
+214
+00:16:36,410 --> 00:16:40,410
+لأنه احنا قلنا ال capsular material water soluble
+
+215
+00:16:40,410 --> 00:16:44,610
+فبالتالي ممكن يروح الكابسول مع ال washing خلال ال
+
+216
+00:16:44,610 --> 00:16:48,530
+washing هعمل air drying و بعدين هشوف على ال oil ال
+
+217
+00:16:48,530 --> 00:16:56,230
+oil immersion النتيجة هاي الشريح الخطوات بالصور
+
+218
+00:16:56,230 --> 00:16:56,910
+قلناها
+
+219
+00:16:59,470 --> 00:17:03,250
+هان بقوللي الـ capsule appear as clear zone الـ
+
+220
+00:17:03,250 --> 00:17:06,990
+capsule بيبين ك clear zone حوالين البكتيريا من
+
+221
+00:17:06,990 --> 00:17:10,190
+الأمثلة على البكتيريا الكابسولات دوحفز استريبتو
+
+222
+00:17:10,190 --> 00:17:14,210
+كوكسي نيومانيا إكليبسيلا نيومانيا والصدو مؤنس
+
+223
+00:17:14,210 --> 00:17:18,330
+نتيجة اللي هي ال capsule stain بطريقة ال genes
+
+224
+00:17:18,330 --> 00:17:22,150
+method ال background انصبغت لونها أسود بال India
+
+225
+00:17:22,150 --> 00:17:27,090
+ink البكتيريا انصبغت باللون الأزرق بال مثلين blue
+
+226
+00:17:27,090 --> 00:17:31,970
+بال basic stainلكن حوالين اللي هي البكتيريا فيها
+
+227
+00:17:31,970 --> 00:17:35,290
+عندي منطقة فاضية Halo area مش بصبوغة لأنه مايلها
+
+228
+00:17:35,290 --> 00:17:40,290
+شحنة هي الـ capsule هذا بالنسبة للـ Gens method
+
+229
+00:17:40,290 --> 00:17:44,370
+الـ Gens method اتعرفنا على ال principle تبع حقس
+
+230
+00:17:44,370 --> 00:17:48,610
+خطوات الصباح وكيف بتكون النتيجة الطريقة التانية
+
+231
+00:17:48,610 --> 00:17:54,060
+اللي هي الانثونيز method الانثونيز methodهعمل سمير
+
+232
+00:17:54,060 --> 00:17:57,460
+بpreparation عادى زى أي ستان مش زى الجنس الستان
+
+233
+00:17:57,460 --> 00:18:00,460
+باختلف لأ الanthonas method هعمل السمير
+
+234
+00:18:00,460 --> 00:18:04,760
+بpreparation عادى هعمل air-drying مش هعمل heat
+
+235
+00:18:04,760 --> 00:18:09,180
+-fixation وذكرنا الصباغة بالنسبة لأ الصباغة هستخدم
+
+236
+00:18:09,180 --> 00:18:13,820
+two reagents أول إشي ال primary stain هيكون ال
+
+237
+00:18:13,820 --> 00:18:17,680
+crystal violet only which gives the bacterial cell
+
+238
+00:18:17,680 --> 00:18:21,750
+and its capsular material a dark purpleبقولّي هى
+
+239
+00:18:21,750 --> 00:18:27,230
+تعطى لون البربول للكبسولر للبكتيريا السلوى
+
+240
+00:18:27,230 --> 00:18:31,090
+الكبسولر material unlike the cell انكمل the
+
+241
+00:18:31,090 --> 00:18:34,630
+capsule is non-ionic and the primary stain cannot
+
+242
+00:18:34,630 --> 00:18:39,110
+adhere بقولّي اتفقنا انه الكبسول non-ionic ماقله
+
+243
+00:18:39,110 --> 00:18:42,430
+شحنة بالتالي ال primary stain ال crystal violet مش
+
+244
+00:18:42,430 --> 00:18:50,470
+هتقدر تلتصق فيهمدت الصباغة في الـCrystal Violet في
+
+245
+00:18:50,470 --> 00:18:54,210
+خطوة الـAnthonys Method بقولّي من 4 لـ7 دقائق
+
+246
+00:18:54,210 --> 00:18:59,470
+بخليها مغمورة بالـCrystal Violet من 4 لـ6 دقائق في
+
+247
+00:18:59,470 --> 00:19:05,450
+الـGram Stain لما أنا صبغت البكتيريا
+
+248
+00:19:05,450 --> 00:19:09,590
+بالـGram Stain استخدمت الـPrimary Stain الـCrystal
+
+249
+00:19:09,590 --> 00:19:12,930
+Violet لكن أنا خلّت الـCrystal Violet one minute
+
+250
+00:19:13,340 --> 00:19:18,060
+لكن هان بقوللي انه انا لازم اخليها من اربع لسبع
+
+251
+00:19:18,060 --> 00:19:22,020
+دقائق بقوللي المدة الطويلة هاي بتكون as mordant
+
+252
+00:19:22,020 --> 00:19:26,220
+يبقى بعد خطوة ال primary stain في عندي mordant ال
+
+253
+00:19:26,220 --> 00:19:31,520
+mordant هان المدة الطويلة للصبغة هتثبت الصبغة داخل
+
+254
+00:19:31,520 --> 00:19:38,930
+البكتيريا بعد ما اناأخلص أتخلص أعمل اللي هو أتخلص
+
+255
+00:19:38,930 --> 00:19:43,150
+الأربع لسبعة دقائق هل هغسل؟ بقول لي مش هتغسلي لأنه
+
+256
+00:19:43,150 --> 00:19:47,150
+احنا قولنا انه الـ Capsular material water soluble
+
+257
+00:19:47,150 --> 00:19:51,510
+فبالتالي ممكن الكابسول هينزل خلال عملية ال washing
+
+258
+00:19:51,510 --> 00:19:56,340
+هيعطيني false negative resultهعمل خطوة الـ
+
+259
+00:19:56,340 --> 00:19:59,720
+Decolorizer الـ Decolorizer بقولّي اللي هو الـ
+
+260
+00:19:59,720 --> 00:20:04,220
+cover sulphate solution 20% نسبته cover sulphate
+
+261
+00:20:04,220 --> 00:20:08,420
+solution نحفظ التركيز في الـ Indus Bar ماري معناه
+
+262
+00:20:08,420 --> 00:20:12,120
+الـ cover salt هي كانت من مكونات الملقاقات الـ
+
+263
+00:20:12,120 --> 00:20:14,820
+green اللي كانت تعتبر water soluble عشان ما انا
+
+264
+00:20:14,820 --> 00:20:18,140
+خربتش هان cover sulphate اللي هو يعتبر الـ
+
+265
+00:20:18,140 --> 00:20:21,620
+Decolorizer في خطوة الـ Anthonys method لـ
+
+266
+00:20:21,620 --> 00:20:25,890
+capsular stampبقولّي هذه المادة الكيميائية الـ
+
+267
+00:20:25,890 --> 00:20:30,830
+copper sulfate بتعمل دور مزدوج Dual roles as both
+
+268
+00:20:30,830 --> 00:20:35,130
+the decolorizing agent and counterstain يبقى
+
+269
+00:20:35,130 --> 00:20:38,950
+الـprimer stain الـcrystal violet الماردنت المدة
+
+270
+00:20:38,950 --> 00:20:44,110
+الطويلة لصبغة من 4 إلى 7 دقائق الـdecolorizer و
+
+271
+00:20:44,110 --> 00:20:49,350
+الـcounterstain هيكون الـ20% copper sulfate طيب،
+
+272
+00:20:49,350 --> 00:20:55,130
+شو هي الوظيفة لـ الـ copper sulfate؟بقول لي إنه
+
+273
+00:20:55,130 --> 00:20:59,030
+يعتبر كـ decolorizer أو ممكن أنا أعتبره washer
+
+274
+00:20:59,030 --> 00:21:01,930
+كمان لأنه مش هستخدم الميه لأنه أحنا قلنا الـ
+
+275
+00:21:01,930 --> 00:21:06,190
+capsular material هي تعتبر water soluble فهيعتبر
+
+276
+00:21:06,190 --> 00:21:10,970
+كمان washer it removes excess primary stain بقول
+
+277
+00:21:10,970 --> 00:21:15,610
+لي هيزيل أي إضافات من ال primary stain إحنا بعد أي
+
+278
+00:21:15,610 --> 00:21:19,050
+صبغة بنغسل عشان أي إش زيادة يروحها مش هستخدم ال
+
+279
+00:21:19,050 --> 00:21:24,080
+water فهو هيغسل كمان يبقى هيستخدم ك washeras well
+
+280
+00:21:24,080 --> 00:21:29,380
+as color from the capsule only and capsule appear
+
+281
+00:21:29,380 --> 00:21:32,600
+faint light blue يبقى خلّينا في هاي كمان بقولك
+
+282
+00:21:32,600 --> 00:21:35,960
+هيزيل اللون من الكابسول اشهر طبيعي اللون هيروح من
+
+283
+00:21:35,960 --> 00:21:41,480
+الكابسول لأن الكابسول مايله شحنة non-ionic طيب هاي
+
+284
+00:21:41,480 --> 00:21:46,040
+وظيفته ك decolorizer أو washer بقولي and capsule
+
+285
+00:21:46,040 --> 00:21:50,340
+appear light blue طيب كيف الكابسول هياخد اللون
+
+286
+00:21:50,340 --> 00:21:55,030
+الأزرق الفاتح وهو مايله شحنةومرتب تطفي الصبغة
+
+287
+00:21:55,030 --> 00:22:01,870
+قولنا الكابر سلفات هيشتغل ك counter stand كيف
+
+288
+00:22:01,870 --> 00:22:07,670
+هيشتغل الكابر سلفات ك counter stand ويعطي الكابسول
+
+289
+00:22:07,670 --> 00:22:11,390
+اللون الأزرق بالرغم من أنه ما إله شحنة بقولي
+
+290
+00:22:11,390 --> 00:22:15,470
+الكابر سلفات هيشتغل ك counter stand من خلال أنه
+
+291
+00:22:15,470 --> 00:22:20,200
+هيعملي فرق في الأيونات يبقىهذا الفرق هيعطي
+
+292
+00:22:20,200 --> 00:22:24,820
+الكابسول بعض من الأيونات يعني هيعطي بعض من الشحنات
+
+293
+00:22:24,820 --> 00:22:29,960
+بالتالي الصبغة هترتبط فيه وهتنطق .. هتطلع خارج
+
+294
+00:22:29,960 --> 00:22:34,600
+الخلية وهيسير للكابسول هتعطيه لون أزرق فأفرح يبقى
+
+295
+00:22:34,600 --> 00:22:40,740
+هيك اشتغل ال .. ال cover solvent كCounter Stain
+
+296
+00:22:40,740 --> 00:22:44,460
+انه اعطى الكابسول اللون ال light blue اشتغل ك
+
+297
+00:22:44,460 --> 00:22:47,920
+decolorizing agent من خلال انه زال اللون من
+
+298
+00:22:47,920 --> 00:22:53,360
+الكابسول يبقى ال counter الكبرسالفات اشتغلي ك
+
+299
+00:22:53,360 --> 00:22:56,540
+counter stain مش يعني counter stain يعني ميزلي
+
+300
+00:22:56,540 --> 00:22:59,900
+حاجة عن حاجة الخلية البكتيرية اعطاها اللون ال
+
+301
+00:22:59,900 --> 00:23:04,100
+purple بشكل طبيعي لكن الكابسول اعطاها اللون الفاتح
+
+302
+00:23:04,100 --> 00:23:09,030
+ال light blue يبقى هيك كان ك counter stainبالنسبة
+
+303
+00:23:09,030 --> 00:23:15,050
+للخطوات أول خطوة قلنا aseptically transfer a loop
+
+304
+00:23:15,050 --> 00:23:18,410
+full of culture within a nicolating loop to the
+
+305
+00:23:18,410 --> 00:23:22,390
+saliva بدي أعمل smear preparation aseptically يعني
+
+306
+00:23:22,390 --> 00:23:26,610
+بدي أمنع أي شيء كنت مانعش أن أستخدم استيرال loop
+
+307
+00:23:26,610 --> 00:23:32,150
+أشتغل في مكان معقّم لو استخدمت مثلا البكتيريا من
+
+308
+00:23:32,150 --> 00:23:36,870
+إبلات تكون ال normal saline استيرال كل هايبسيبتك
+
+309
+00:23:36,870 --> 00:23:40,290
+تكنيك عشان امنع الcontamination الخطوة التانية
+
+310
+00:23:40,290 --> 00:23:45,050
+هعمل air drying بعدها مش هعمل heat fixation لان
+
+311
+00:23:45,050 --> 00:23:52,270
+اتفقنا ان هو fragile ايش ال fragile يعني الكابسول
+
+312
+00:23:52,270 --> 00:23:56,490
+ممكن انه يتكسر بالحرارة فبالتالي يعطيني false
+
+313
+00:23:56,490 --> 00:24:02,690
+negative result في سبب تاني انهليش أنا بديش اعمل
+
+314
+00:24:02,690 --> 00:24:06,950
+heat fixation في ال slide الأبل in this procedure
+
+315
+00:24:06,950 --> 00:24:11,410
+السمير shouldn't be hit heat fixed since shrinkage
+
+316
+00:24:11,410 --> 00:24:15,210
+is likely to occur and create a clear zone around
+
+317
+00:24:15,210 --> 00:24:19,070
+the bacteria which can be mistaken for a capsule
+
+318
+00:24:19,070 --> 00:24:24,110
+بقوللي انا لو انا روحت عملت heat fixation ممكن
+
+319
+00:24:24,110 --> 00:24:28,590
+الخلية البكتيرية يصير لها shrinkingلما تنكمش
+
+320
+00:24:28,590 --> 00:24:32,190
+الخلية البكتيرية هيكون في مكان فاضي create clear
+
+321
+00:24:32,190 --> 00:24:36,790
+zone around the bacterium فممكن أنا أفكر ال clear
+
+322
+00:24:36,790 --> 00:24:40,970
+zone هذا هو الكابسول لكن مش كابسول هيعطيني false
+
+323
+00:24:40,970 --> 00:24:44,810
+positive result يبقى في عندي سببين لعدم عمل خطوة
+
+324
+00:24:44,810 --> 00:24:48,650
+ال heat fixation السبب الأول لأنه fragile الكابسول
+
+325
+00:24:48,650 --> 00:24:51,970
+فبالتالي ممكن يتكسر بالحرارة ويعطيني false
+
+326
+00:24:51,970 --> 00:24:56,360
+negative result لكن السبب التانيممكن البكتيريا
+
+327
+00:24:56,360 --> 00:24:59,560
+يصير لها shrinking فبالتالي تكون الـ clear zone
+
+328
+00:24:59,560 --> 00:25:04,160
+أفكره هو الكابسول فهي يعطيني false positive result
+
+329
+00:25:04,160 --> 00:25:10,120
+نكمل الخطوات الخطوة اللي بعدها أعمل staining بالـ
+
+330
+00:25:10,120 --> 00:25:12,780
+Primer Stain اللي هي الـ crystal violet يبقى ال
+
+331
+00:25:12,780 --> 00:25:16,340
+crystal violet هي الـ Primer Stain هخليها من أربع
+
+332
+00:25:16,340 --> 00:25:20,000
+لسبع دقائق وقولنا هي الأربع لسبع دقائق هتكون
+
+333
+00:25:20,000 --> 00:25:25,270
+الموردنت عشرين في الميةالخطوة التي سأقوم بها هي كـ
+
+334
+00:25:25,270 --> 00:25:29,270
+Decalarizer أو Washer وهي نفس الشيء كـ Counter
+
+335
+00:25:29,270 --> 00:25:32,970
+Stain إذا ملاحظين ما كان في خطوة اللي هو هغسل
+
+336
+00:25:32,970 --> 00:25:36,090
+بالميه لأن احنا قلنا الـ Capsular Material هي
+
+337
+00:25:36,090 --> 00:25:40,990
+تعتبر Water Soluble هعمل Air Drying و هشوفها تحت
+
+338
+00:25:40,990 --> 00:25:45,970
+الميكروسكوب على الـ Oil Emergent يبقى نكتب
+
+339
+00:25:45,970 --> 00:25:49,830
+الـCrystal
+
+340
+00:25:49,830 --> 00:25:50,490
+Violet
+
+341
+00:25:54,140 --> 00:25:57,560
+الاربع عال سبعة هتكون مواردانت والعشرين في المية
+
+342
+00:25:57,560 --> 00:26:04,980
+هيكون الكبر سلفات هيكون decalarizer and
+
+343
+00:26:04,980 --> 00:26:07,300
+counterstain
+
+344
+00:27:30,620 --> 00:27:34,540
+يبقى في هاي طريقة الانثوناز method تعرفنا على ال
+
+345
+00:27:34,540 --> 00:27:37,960
+primary stain الموردانت ال decolorizer and ال
+
+346
+00:27:37,960 --> 00:27:41,660
+counter stain في طريقة ال genes method ما استخدمت
+
+347
+00:27:41,660 --> 00:27:44,520
+ولا primary ولا موردانت ولا decolorizer أو counter
+
+348
+00:27:44,520 --> 00:27:48,660
+stain كانتالفكرة إنه أنا بدأ أستخدم أسدكستان كانت
+
+349
+00:27:48,660 --> 00:28:05,300
+أوبازاكستان فبالتالي هيبين عندي ال capsule هذه
+
+350
+00:28:05,300 --> 00:28:11,140
+الصورة بتوضح بعض الخطوات في الصورة قلنا crystal
+
+351
+00:28:11,140 --> 00:28:14,160
+violet primary stain الأربع على سبعة minutes هتكون
+
+352
+00:28:14,160 --> 00:28:19,030
+more thanبعدها مش هغسل بالميه هعمل اللى هغسل بيه
+
+353
+00:28:19,030 --> 00:28:22,030
+ال cover salivate عشرين في الميه cover salivate
+
+354
+00:28:22,030 --> 00:28:27,030
+هتكون كخطوط ال decalarizer و ال counter stand هذا
+
+355
+00:28:27,030 --> 00:28:31,310
+بالنسبة لنتائج اللى هى ال capsular stand باستخدام
+
+356
+00:28:31,310 --> 00:28:35,950
+طريقة ال anthonis method ال capsule منلاحظ أن هو
+
+357
+00:28:36,540 --> 00:28:40,540
+البكتيريا ماخدة اللون الـ dark purple عادي لأنه
+
+358
+00:28:40,540 --> 00:28:43,660
+هتاخد الـ crystal violet بشكل طبيعي لأنه هي basic
+
+359
+00:28:43,660 --> 00:28:48,160
+stain هتصبغ البكتيريا الصالبة الكابسول هياخد لون
+
+360
+00:28:48,160 --> 00:28:52,000
+فاتح كتير لون موف فاتح او ممكن نعتبره أزرق فاتح
+
+361
+00:28:52,000 --> 00:28:55,520
+لأنه احنا قلنا الكبر سلفات هيعملي فرق في الأيونات
+
+362
+00:28:55,520 --> 00:28:59,340
+هيعطي الكابسول ايونات يعني هيصيرله شحنة فبالتالي
+
+363
+00:28:59,340 --> 00:29:05,080
+هتطلع جزء من الصبغة لخرج البكتيريا وهتتلون باللون
+
+364
+00:29:05,430 --> 00:29:09,450
+الـ Light Blue أو الـ Light Purple بدنا نفرق
+
+365
+00:29:09,450 --> 00:29:13,070
+بنتيجة الـ Anthony's method والـ Gen's method ال
+
+366
+00:29:13,070 --> 00:29:16,790
+result بتختلف حسب الطريقة بدنا نكون عارفين كل
+
+367
+00:29:16,790 --> 00:29:22,010
+طريقة شو ال principle تبعها ايش ال procedure لكل
+
+368
+00:29:22,010 --> 00:29:27,250
+طريقة وكيف بتكون النتيجة في كل طريقة هذا بالنسبة
+
+369
+00:29:27,250 --> 00:29:28,970
+لـ Capsular Style
+
diff --git a/PL9fwy3NUQKwZWgAb_V1ATlRRsH-6i3cPC/XkWBgngbjy4_raw.srt b/PL9fwy3NUQKwZWgAb_V1ATlRRsH-6i3cPC/XkWBgngbjy4_raw.srt
new file mode 100644
index 0000000000000000000000000000000000000000..3f3b9aa2ddc40394983563ec53aba2a27683a7dc
--- /dev/null
+++ b/PL9fwy3NUQKwZWgAb_V1ATlRRsH-6i3cPC/XkWBgngbjy4_raw.srt
@@ -0,0 +1,1476 @@
+1
+00:00:00,690 --> 00:00:03,830
+تاني نوع من أنواع الـ special stain الـ capsular
+
+2
+00:00:03,830 --> 00:00:06,490
+stain قلنا إنه الـ capsule أحد الـ special
+
+3
+00:00:06,490 --> 00:00:09,770
+component اللي عدم وجودها لا يؤثر على الخلية
+
+4
+00:00:09,770 --> 00:00:13,190
+البكتيرية يعني في عندي بكتيريا capsulated وفي
+
+5
+00:00:13,190 --> 00:00:17,470
+بكتيريا non-capsulated capsule are gelatinous
+
+6
+00:00:17,470 --> 00:00:22,010
+outer layer structure هو طبقة خارجية secreted by
+
+7
+00:00:22,010 --> 00:00:25,730
+bacterial cells that lay outside of an organism
+
+8
+00:00:25,730 --> 00:00:30,060
+cell wallبكون خارج الخلية البكتيرية and these are
+
+9
+00:00:30,060 --> 00:00:32,900
+in direct contact with the environment فبالتالي
+
+10
+00:00:32,900 --> 00:00:36,680
+هيكون ملامس للبيئة الخارجية الـ capsule بيتم
+
+11
+00:00:36,680 --> 00:00:40,540
+تصنيعه فيه الـ cytoplasm and secret to the outside
+
+12
+00:00:40,540 --> 00:00:45,240
+of the cell وبيطلع خارج الخلية البكتيرية where it
+
+13
+00:00:45,240 --> 00:00:49,340
+surrounds the bacteria وبحيط في هذه الخليةmany
+
+14
+00:00:49,340 --> 00:00:53,700
+perhaps most bacteria produce capsule under the
+
+15
+00:00:53,700 --> 00:00:56,860
+right condition بيقوللي معظم البكتيريا بتكون
+
+16
+00:00:56,860 --> 00:01:00,320
+الكبسول في ال right condition إيش يعني في ال right
+
+17
+00:01:00,320 --> 00:01:03,120
+condition؟ يعني لو كانت الظروف مناسبة أو كان في
+
+18
+00:01:03,120 --> 00:01:08,860
+عندي مغذيات المغذيات هي الفائض من المغذيات بتتخزن
+
+19
+00:01:08,860 --> 00:01:13,040
+في الكبسول من وظائف الكبسول إنه يعتبر مخزن للمواد
+
+20
+00:01:13,040 --> 00:01:16,100
+الغذائية في حال كان في عندي فائض
+
+21
+00:01:19,770 --> 00:01:25,650
+Capsules are fragile بقولّي إن الكبسول هش and can
+
+22
+00:01:25,650 --> 00:01:30,370
+be dimensioned dissociated, destroyed or destroyed
+
+23
+00:01:30,370 --> 00:01:34,550
+by heat بقولّي إن الكبسول if fragile فبالتالي
+
+24
+00:01:34,550 --> 00:01:40,310
+هيتقلص و هيصير جاف و هيتشوّه و يتدمر بواسطة
+
+25
+00:01:40,310 --> 00:01:44,950
+الحرارة من خطوات لستان قلنا إن في عندي أول إشسمير
+
+26
+00:01:44,950 --> 00:01:49,540
+preparation air drying heat fixationبما أن
+
+27
+00:01:49,540 --> 00:01:53,660
+الكابسول إفراجيل وبتكسر بواسطة الحرارة يبقى خطوة
+
+28
+00:01:53,660 --> 00:01:58,360
+الـ heat fixation في الكابسولارستان مش هعملها لأنه
+
+29
+00:01:58,360 --> 00:02:02,400
+هيدمر الكابسول من خلال هذه الخطوة لو أنا عملتها
+
+30
+00:02:02,400 --> 00:02:08,060
+فبالتالي هيعطيني false result most capsule are
+
+31
+00:02:08,060 --> 00:02:13,440
+composed of polysaccharide بقولي معظم البكتيريا
+
+32
+00:02:13,440 --> 00:02:16,880
+اللي بتكون كابسول هي كابسول عبارة عن
+
+33
+00:02:16,880 --> 00:02:21,370
+polysaccharidebut some are composed of polypeptide
+
+34
+00:02:21,370 --> 00:02:25,750
+لكن في بعض البكتيريا الكابسول تبعها polypeptide زي
+
+35
+00:02:25,750 --> 00:02:31,170
+البصيلاس أنتراكسي بيوكايد كولوني showing viscous
+
+36
+00:02:31,170 --> 00:02:35,110
+or sticky growth typical of an organism producing
+
+37
+00:02:35,110 --> 00:02:39,090
+large quantities of a carbohydrate capsule قولنا
+
+38
+00:02:39,090 --> 00:02:43,470
+معظم البكتيريا اللي بتكون كابسول الكولوني تبعها
+
+39
+00:02:43,470 --> 00:02:46,210
+على الطبق في ان احنا كولوني morphology ممكن من
+
+40
+00:02:46,210 --> 00:02:51,210
+خلاله اتعرف على البكتيرياالمصدع مرات على الطبق بعد
+
+41
+00:02:51,210 --> 00:02:54,430
+ما أنا أنمي هذه البكتيريا بتكون طبيعتها Viscous
+
+42
+00:02:54,430 --> 00:02:57,830
+بتكون لذجة على سبيل المثال الكليبسيلة تعتبر
+
+43
+00:02:57,830 --> 00:03:02,510
+capsulated لما تيجي تاخدي كولوني كليبسيلة باللوب
+
+44
+00:03:02,510 --> 00:03:06,410
+بتلاقيها بتمغط هذا نتيجة الكميات الكبيرة من
+
+45
+00:03:06,410 --> 00:03:09,970
+الكربوهيدرات اللي بتكون منها الكابسول قولنا ان
+
+46
+00:03:09,970 --> 00:03:12,330
+الكابسول polysaccharide يعني carbohydrate
+
+47
+00:03:15,880 --> 00:03:20,620
+Capsules function شو وظائف الكبسول أول وظيفة
+
+48
+00:03:20,620 --> 00:03:24,740
+protect the cell from drying هيحميل البكتيريا من
+
+49
+00:03:24,740 --> 00:03:29,180
+الجفاف الكبسول هيعمل inhibit لالwater from
+
+50
+00:03:29,180 --> 00:03:33,160
+escaping into environment فبالتالي هيحمي الخلية
+
+51
+00:03:33,160 --> 00:03:37,280
+البكتيرية من الجفاف protect the cell from
+
+52
+00:03:37,280 --> 00:03:42,070
+phagocyte being engulfed by white blood cellبيحمي
+
+53
+00:03:42,070 --> 00:03:46,890
+الخلية من الـ phagocytosis لأن الكبسول لذج فسكس
+
+54
+00:03:46,890 --> 00:03:50,490
+فلما تيجي الـ phagocyte اللي هي الـ White BCs عشان
+
+55
+00:03:50,490 --> 00:03:55,590
+تعمل Engulfment تلتهم الخلية بتروح الخلية بتهرب
+
+56
+00:03:55,590 --> 00:04:01,050
+لأن الخلية بتحتوي على الكبسول اللذج فتهرب من جهاز
+
+57
+00:04:01,050 --> 00:04:04,510
+المناعة فتهرب من الـ White BCs وهذا يعتبر
+
+58
+00:04:04,510 --> 00:04:09,460
+Virulence Factor عامل من عوامل الشرسةتالت وظيفة
+
+59
+00:04:09,460 --> 00:04:12,840
+provide a food reserve when a certain organic
+
+60
+00:04:12,840 --> 00:04:16,980
+compounds are in excess بيقول يعتبر مخزن للمواد
+
+61
+00:04:16,980 --> 00:04:21,700
+الغذائية في حال عندي كان فائض من هذه المواد هتتخزن
+
+62
+00:04:21,700 --> 00:04:26,460
+في الكابسول رابع وظيفة يعتبر قولنا ان هو virulence
+
+63
+00:04:26,460 --> 00:04:30,040
+factor عامل من عوامل الشرازة of pathogenic
+
+64
+00:04:30,040 --> 00:04:35,760
+microbes خامس وظيفةthey serve as binding or
+
+65
+00:04:35,760 --> 00:04:39,380
+adhesion agent for sticking cell together or to
+
+66
+00:04:39,380 --> 00:04:44,200
+surface وقوللي الوظيفة الخامسة هي ال attachment ال
+
+67
+00:04:44,200 --> 00:04:47,800
+attachment ممكن تلتثق الخلايا البكتيرية مع بعض
+
+68
+00:04:47,800 --> 00:04:52,440
+together or to the surface أو على السطح الكابسول
+
+69
+00:04:52,440 --> 00:04:56,740
+لذج فبالتالي هتلذق البكتيريا على بعض مع بعض أو على
+
+70
+00:04:56,740 --> 00:05:01,100
+السطحطيب شوف فايدة ال attachment بقول عشان يصير في
+
+71
+00:05:01,100 --> 00:05:05,840
+عندي infection عشان يصير المرض أول خطوة لازم يصير
+
+72
+00:05:05,840 --> 00:05:10,120
+ال attachment تلتثق البكتيريا مع بعض أو تلتثق على
+
+73
+00:05:10,120 --> 00:05:14,420
+السطح بعدين هيصير في عندي colonization بعدين هيصير
+
+74
+00:05:14,420 --> 00:05:19,590
+ال infectionممكن قلنا يكون على الأسطح زي السخور أو
+
+75
+00:05:19,590 --> 00:05:23,090
+على سطح الأسنان في عندي بكتيريا الـ Streptococcus
+
+76
+00:05:23,090 --> 00:05:27,350
+mutans هذه البكتيريا بتعمل تسوص الأسنان لأنه
+
+77
+00:05:27,350 --> 00:05:35,330
+موجودة في الفم في وجود السكراسة تنتج الكابسول هذه
+
+78
+00:05:35,330 --> 00:05:38,810
+ال slide هنتكلم عن عوامل لازم أنا أخدها بعين
+
+79
+00:05:38,810 --> 00:05:43,940
+الاعتبار لما بدي أصبغ الكابسولأول معلومة بكتيريا
+
+80
+00:05:43,940 --> 00:05:49,180
+الكابسول are non-ionic قولي ان الكابسول مايله شحنة
+
+81
+00:05:49,180 --> 00:05:54,380
+so neither acidic nor basic stain will adhere to
+
+82
+00:05:54,380 --> 00:05:57,800
+their surface عشان هيك لا ال acidic stain ولا ال
+
+83
+00:05:57,800 --> 00:06:03,500
+basic stain هتقدر تلتثق بالكابسول هتبع هعمل طريقة
+
+84
+00:06:03,500 --> 00:06:06,840
+عشان اقدر اني انا اصبغ الكابسول هنتعرف عليها
+
+85
+00:06:06,840 --> 00:06:11,240
+because most capsule material are water soluble
+
+86
+00:06:11,640 --> 00:06:15,320
+simple stain will not adhere to them بقول لي معظم
+
+87
+00:06:15,320 --> 00:06:18,980
+مكونات الكابسول هي water soluble عشان هيك ال
+
+88
+00:06:18,980 --> 00:06:23,980
+simple stain مابتقدر تلتصق بالكابسول ال simple
+
+89
+00:06:23,980 --> 00:06:28,820
+stain أنا خلال ال simple stain بعمل washing بين كل
+
+90
+00:06:28,820 --> 00:06:33,040
+خطوة والتانية فبالتالي الكابسول material هيتم
+
+91
+00:06:33,040 --> 00:06:36,700
+إزالته خلال ال washing يبقى خلال شغل في الكابسول
+
+92
+00:06:36,700 --> 00:06:41,120
+stainبدي اخد بيعني الاعتبار rinsing the slide with
+
+93
+00:06:41,120 --> 00:06:45,000
+water لازم اني انا اتجنبه اني اغسل ال slide بالميه
+
+94
+00:06:45,000 --> 00:06:50,000
+لان الكابسول هيصله ازالة dislodge الكابسول هيتم
+
+95
+00:06:50,000 --> 00:06:57,700
+ازالته خلال عملية ال washing تالت معلومة ال older
+
+96
+00:06:57,700 --> 00:07:01,780
+culture are most likely to exhibit capsule
+
+97
+00:07:01,780 --> 00:07:06,490
+production بقوللي انه لما بدي انا اعملكابسول استان
+
+98
+00:07:06,490 --> 00:07:10,310
+باخد الـ colony من all their culture ليش؟ لأن الـ
+
+99
+00:07:10,310 --> 00:07:13,310
+all .. لأن في الـ all their culture بتكون المواد
+
+100
+00:07:13,310 --> 00:07:18,010
+الغذائية بدأت تتخلص و الظروف بتصبح سيئة فبالتالي
+
+101
+00:07:18,010 --> 00:07:22,730
+هيتكون عندي الكابسول نفس الاشي بالنسبة للـ Spar
+
+102
+00:07:22,730 --> 00:07:27,130
+يفضل أنها تكون all their culture لأن بتتكون
+
+103
+00:07:27,130 --> 00:07:32,070
+الكابسول و الـ Spar في .. لما تتصير الظروف البيئية
+
+104
+00:07:32,070 --> 00:07:37,040
+صعبةWhen performing a capsule stand on your
+
+105
+00:07:37,040 --> 00:07:40,740
+unknown, be sure the culture you take your sample
+
+106
+00:07:40,740 --> 00:07:44,980
+from is at least five days old.أقولك عشان هيك لما
+
+107
+00:07:44,980 --> 00:07:50,040
+بدك أنتي تعملي أي capsule stand، تاخدي الخلوني من
+
+108
+00:07:50,040 --> 00:07:56,810
+culture، من مزرعة على الأقل يكون لها خمس أيامأتراب
+
+109
+00:07:56,810 --> 00:08:01,590
+من السيرام يمكن استخدامه في تحريك لتحسين صميم
+
+110
+00:08:01,590 --> 00:08:07,750
+الكبسول وتجعله أسهل للتلاعب مع كمباون عادل مثل
+
+111
+00:08:07,750 --> 00:08:14,420
+ميكروسكوب ضوء وقلي أثناء عمليلأ لسمير في الكابسول
+
+112
+00:08:14,420 --> 00:08:19,100
+الستان ممكن اضيف drop of serum drop of serum بقول
+
+113
+00:08:19,100 --> 00:08:23,760
+هذا هيعمل على زيادة حجم الكابسول enhance the size
+
+114
+00:08:23,760 --> 00:08:26,480
+of the capsule يبقى هي وضيفت اللي هو ال serum
+
+115
+00:08:26,480 --> 00:08:31,700
+فبالتالي انا هقدر اشوف الكابسول بشكل واضح وقت اش
+
+116
+00:08:31,700 --> 00:08:35,500
+بحط اللي هو drop of serum عندنا هنتعرف خلال السمير
+
+117
+00:08:35,500 --> 00:08:40,920
+انا بضيف كولوني و هضيف معه اللي هو drop of serum
+
+118
+00:08:44,070 --> 00:08:47,990
+كيف بدي انا اصبغ الكابسول في عندي انا طريقتين
+
+119
+00:08:47,990 --> 00:08:51,670
+لصباغة الكابسول ال acid fast اذا بتتذكروا باصل
+
+120
+00:08:51,670 --> 00:08:56,910
+لايكان في عندي تلك طرق لصباغتها بدي انا اكون عارفة
+
+121
+00:08:56,910 --> 00:08:59,990
+كل الطرق و شو ال principle لكل طريقة و كيف بتكون
+
+122
+00:08:59,990 --> 00:09:04,550
+نتيجة كل منها بالنسبة لالكابسول أستان اول طريقة
+
+123
+00:09:04,550 --> 00:09:10,390
+اسمها الgens stand method بشكل مختصر احنا قلنا انه
+
+124
+00:09:10,390 --> 00:09:14,930
+الكابسول non-ionic مايله شحنةطب انا كيف بدي أصبغ
+
+125
+00:09:14,930 --> 00:09:19,270
+هذا الـ capsule بقولنا في هذه الطريقة بستخدم نوعين
+
+126
+00:09:19,270 --> 00:09:24,050
+من الصبغار بستخدم basic stain و بستخدم acidic
+
+127
+00:09:24,050 --> 00:09:28,650
+stain ال basic stain هتصبغ البكتيريا بستخدم basic
+
+128
+00:09:28,650 --> 00:09:32,570
+stain زي ال methylene blue أو ال crystal violet ال
+
+129
+00:09:32,570 --> 00:09:35,610
+acidic stain هتصبغ قولنا بتتذكروا في معمل ال
+
+130
+00:09:35,610 --> 00:09:37,730
+symbolism بتصبغ ال background
+
+131
+00:09:40,530 --> 00:09:43,830
+من الأمثلة على الـ Acidic Stain اللي ممكن استخدمها
+
+132
+00:09:43,830 --> 00:09:48,410
+زي النكروسين أو الانتياينك يبقى ال basic stain
+
+133
+00:09:48,410 --> 00:09:53,350
+صبغت البكتيريا ال acidic stain هتصبغها الخلفية ال
+
+134
+00:09:53,350 --> 00:09:57,630
+background بالتالي هيبين الكابسول على شكل hello
+
+135
+00:09:57,630 --> 00:10:02,670
+area على شكل فجوات حول البكتيريا هيقدرت أعرف هل هي
+
+136
+00:10:02,670 --> 00:10:06,650
+البكتيريا capsulated أو non capsulated بالرغم من
+
+137
+00:10:06,650 --> 00:10:11,710
+أن الكابسول ما له شحنة وما بقدر أصبغهعشان هيك بسمي
+
+138
+00:10:11,710 --> 00:10:16,650
+الـ Capsular Stain بسميها Negative Stain إيش يعني
+
+139
+00:10:16,650 --> 00:10:21,590
+Negative Stain؟ يعني مش هتقدر تاخد الصبغة لأنه
+
+140
+00:10:21,590 --> 00:10:25,450
+مايلها شحنة مش هتقدر تاخد الصبغة سواء كانت Acidic
+
+141
+00:10:25,450 --> 00:10:30,230
+أو Basic Stain مش عشان الكابسولة بيحمل شحنة سالبة
+
+142
+00:10:30,230 --> 00:10:33,070
+روحت سماتها Negative Stain لأ Negative Stain يعني
+
+143
+00:10:33,070 --> 00:10:40,040
+ماتقدر تاخد الصبغة لأنه مايلها شحنةوبهذا السؤال
+
+144
+00:10:40,040 --> 00:10:44,480
+مهم جدا ليش أنا بسمي ال capsular stain ب negative
+
+145
+00:10:44,480 --> 00:10:48,760
+stain نقرا
+
+146
+00:10:48,760 --> 00:10:52,620
+ال slide جينز method هانكتبلي ال principle تبعت
+
+147
+00:10:52,620 --> 00:10:56,660
+هاي الطريقة in this stain we used acidic and basic
+
+148
+00:10:56,660 --> 00:11:01,700
+dye بستخدم acidic و basic dye acidic dye as india
+
+149
+00:11:01,700 --> 00:11:04,740
+ink and negrosin بقولي من الأمثلة على ال acidic
+
+150
+00:11:04,740 --> 00:11:09,000
+dye ال india ink و ال negrosinUse to stain
+
+151
+00:11:09,000 --> 00:11:12,440
+background of the slide بقولي بتستخدم لصباغة ال
+
+152
+00:11:12,440 --> 00:11:17,180
+background and but basic stain ال basic stain زي
+
+153
+00:11:17,180 --> 00:11:20,080
+ال methylene blue أو ال crystal violet هتصبغ
+
+154
+00:11:20,080 --> 00:11:25,860
+الخلية البكتيرية بقولي ال India ink هتعطي semi
+
+155
+00:11:25,860 --> 00:11:30,180
+opaque background هتعطي الخلفية إشي معتم بالمقارنة
+
+156
+00:11:30,180 --> 00:11:34,620
+إنه الكابسول هيك هيبين هيكون واضح وهقدر أني أنا
+
+157
+00:11:34,620 --> 00:11:37,360
+أشوفه بالرغم من إنه ما له شحنة
+
+158
+00:11:42,080 --> 00:11:46,580
+بالنسبة لخطوات الشغل اول اش هعمل smear preparation
+
+159
+00:11:46,580 --> 00:11:50,720
+طريقة الـsmear preparation في الـgens method
+
+160
+00:11:50,720 --> 00:11:55,100
+هتختلف عن اي smear preparation تانية بقوللي بجيب
+
+161
+00:11:55,100 --> 00:11:59,960
+slide بحط على طرف ال slide مش بالنص على الطرف ال
+
+162
+00:11:59,960 --> 00:12:03,280
+slide drop of acidic acid
+
+163
+00:12:08,580 --> 00:12:13,300
+الـ Drop of India Ink أو ممكن نحكي بشكل عام اللي
+
+164
+00:12:13,300 --> 00:12:27,240
+هي أسادكستان أسادكستان
+
+165
+00:12:27,240 --> 00:12:31,560
+سواء India Ink أو نيجروزين بحط drop من هذه الصبغة
+
+166
+00:12:31,560 --> 00:12:36,780
+على one end of the slide على طرف ال slide بروح
+
+167
+00:12:36,780 --> 00:12:41,260
+بجيباللي هو Loop full of bacteria البكتيريا اللي
+
+168
+00:12:41,260 --> 00:12:46,280
+بدي أشوف هل هي فيها كابسول أو لأ وبعمل mix منيح
+
+169
+00:12:46,280 --> 00:12:50,140
+بين ال drop high وما بين البكتيريا لكن بدون ما
+
+170
+00:12:50,140 --> 00:12:53,760
+أعمل توزيع لل drop في نفس المكان بعمل mix
+
+171
+00:13:01,510 --> 00:13:06,310
+بعدها بروح بعد ما اعمل مكسة منيح بجيب slide تاني و
+
+172
+00:13:06,310 --> 00:13:09,550
+زي ال blood smear زي ما كنتوا بتعمله في ال blood
+
+173
+00:13:09,550 --> 00:13:13,610
+smear كنتوا تحطوا drop من ال blood و تفرده هى نفس
+
+174
+00:13:13,610 --> 00:13:17,530
+الشيء لكن هى مش drop of blood drop من الصبغة و من
+
+175
+00:13:17,530 --> 00:13:20,350
+البكتيريا انا عاملها ال home mix بروح بجيب slide
+
+176
+00:13:20,350 --> 00:13:27,140
+على الطرفوبستنى طبعا لما ال drop تيجى على حفة ال
+
+177
+00:13:27,140 --> 00:13:32,120
+slide وبعدين برجع لورا بعدين بعمل smear ما بعرف
+
+178
+00:13:32,120 --> 00:13:38,080
+اذا في عندي صورة هاي
+
+179
+00:13:38,080 --> 00:13:42,400
+الصورة اول اشي بجيب اللى هو drop من ال acidic
+
+180
+00:13:42,400 --> 00:13:46,320
+stand سواء كانت نيجروزين انديا انج او هانذكرلى ال
+
+181
+00:13:46,320 --> 00:13:50,060
+Congo red stand بجيب ال organism اللى انا بدي
+
+182
+00:13:50,380 --> 00:13:54,160
+أفحصله البكتيريا عنده هل هي capsulated أو non
+
+183
+00:13:54,160 --> 00:13:59,620
+-capsulated باجي بـ slide تانية تكون نظيفة و باجي
+
+184
+00:13:59,620 --> 00:14:05,040
+على بالنص بعدها برجع لورا باستنى لل drop انها تيجي
+
+185
+00:14:05,040 --> 00:14:10,600
+على العلى الشريحة على طرف الشريحة الاقيها انها
+
+186
+00:14:10,600 --> 00:14:16,820
+اتوزعت بعدين بقدم لقدام عشان اعمل blood smear عشان
+
+187
+00:14:16,820 --> 00:14:20,720
+اعمل فيلم من اللي هو طبعا هذا فيلم من ال blood لكن
+
+188
+00:14:20,720 --> 00:14:26,320
+هان انا بعمل اللي هو smear ل ال acidic stain مع
+
+189
+00:14:26,320 --> 00:14:30,620
+البكتيريا هلاحظ ان المنطقة القريبة اللي كانت من ال
+
+190
+00:14:30,620 --> 00:14:35,660
+drop هتكون thick وهي المنطقة هتكون thin يبقى هاي
+
+191
+00:14:35,660 --> 00:14:36,940
+أول خطوة نرجع
+
+192
+00:14:39,730 --> 00:14:43,910
+خلصنا من أول خطوة اللى هى الـ Smear preparation
+
+193
+00:14:43,910 --> 00:14:48,150
+الـ Smear preparation قلنا اضرب من الاسدكستان مع
+
+194
+00:14:48,150 --> 00:14:53,450
+البكتيريا وبعمللهم سمير فرد زى طريقة ال blood
+
+195
+00:14:53,450 --> 00:14:57,930
+smearهذه الطريقة انا لما اروح اجيب الاسدكستان و
+
+196
+00:14:57,930 --> 00:15:02,170
+اعملها mix و افرض بزي ال blood سمير بضمن ان ال
+
+197
+00:15:02,170 --> 00:15:06,310
+background كلها انسبغط لان ممكن لو فردت بال loop
+
+198
+00:15:06,310 --> 00:15:10,030
+زي ما كنا نفرض بالسمير العادى ممكن ما اتصل
+
+199
+00:15:10,030 --> 00:15:14,710
+الاسدكستان لكل البكتيريا فبالتالي مش هقدر اشوف ال
+
+200
+00:15:14,710 --> 00:15:18,750
+capsule الخطوة التانية بعد السمير preparation هعمل
+
+201
+00:15:18,750 --> 00:15:23,980
+air dryingهل هعمل heat fixation بقولنا انه ممنوع
+
+202
+00:15:23,980 --> 00:15:28,060
+انا اعمل heat fixation السبب الأول لأنه ال capsule
+
+203
+00:15:28,060 --> 00:15:32,580
+if fragile فبالتالي ممكن يتكسر مع الحرارة ويعطيني
+
+204
+00:15:32,580 --> 00:15:37,500
+false result في عندي سبب تاني هنذكره لما قمر معانا
+
+205
+00:15:37,500 --> 00:15:47,560
+خلال ال slide بعد ما اعملالخطوة التانية هي أصبغ
+
+206
+00:15:47,560 --> 00:15:53,460
+بـ Basic Stain
+
+207
+00:15:53,460 --> 00:15:56,120
+بعد الـ air-drying
+
+208
+00:16:10,530 --> 00:16:14,650
+هنا ذاكر مثليني بلو ممكن اكريستال فيوليت بضيف
+
+209
+00:16:14,650 --> 00:16:19,110
+المثليني بلو على الاسمير بعد ما عملتله air drying
+
+210
+00:16:19,110 --> 00:16:23,970
+لمدة من اتنين لتلاتة minute من اتنين لتلت دقائق
+
+211
+00:16:23,970 --> 00:16:28,250
+بخليها في المثليني بلو بعد ما يخلصوا التلت دقائق
+
+212
+00:16:28,250 --> 00:16:31,890
+بقول لي rinse gently with water and allow to air
+
+213
+00:16:31,890 --> 00:16:36,410
+dryبعدها بغسل بالميه لكن بنحاول انه يكون gently
+
+214
+00:16:36,410 --> 00:16:40,410
+لأنه احنا قلنا ال capsular material water soluble
+
+215
+00:16:40,410 --> 00:16:44,610
+فبالتالي ممكن يروح الكابسول مع ال washing خلال ال
+
+216
+00:16:44,610 --> 00:16:48,530
+washing هعمل air drying و بعدين هشوف على ال oil ال
+
+217
+00:16:48,530 --> 00:16:56,230
+oil immersion النتيجة هاي الشريح الخطوات بالصور
+
+218
+00:16:56,230 --> 00:16:56,910
+قلناها
+
+219
+00:16:59,470 --> 00:17:03,250
+هان بقوللي الـ capsule appear as clear zone الـ
+
+220
+00:17:03,250 --> 00:17:06,990
+capsule بيبين ك clear zone حوالين البكتيريا من
+
+221
+00:17:06,990 --> 00:17:10,190
+الأمثلة على البكتيريا الكابسولات دوحفز استريبتو
+
+222
+00:17:10,190 --> 00:17:14,210
+كوكسي نيومانيا إكليبسيلا نيومانيا والصدو مؤنس
+
+223
+00:17:14,210 --> 00:17:18,330
+نتيجة اللي هي ال capsule stain بطريقة ال genes
+
+224
+00:17:18,330 --> 00:17:22,150
+method ال background انصبغت لونها أسود بال India
+
+225
+00:17:22,150 --> 00:17:27,090
+ink البكتيريا انصبغت باللون الأزرق بال مثلين blue
+
+226
+00:17:27,090 --> 00:17:31,970
+بال basic stainلكن حوالين اللي هي البكتيريا فيها
+
+227
+00:17:31,970 --> 00:17:35,290
+عندي منطقة فاضية Halo area مش بصبوغة لأنه مايلها
+
+228
+00:17:35,290 --> 00:17:40,290
+شحنة هي الـ capsule هذا بالنسبة للـ Gens method
+
+229
+00:17:40,290 --> 00:17:44,370
+الـ Gens method اتعرفنا على ال principle تبع حقس
+
+230
+00:17:44,370 --> 00:17:48,610
+خطوات الصباح وكيف بتكون النتيجة الطريقة التانية
+
+231
+00:17:48,610 --> 00:17:54,060
+اللي هي الانثونيز method الانثونيز methodهعمل سمير
+
+232
+00:17:54,060 --> 00:17:57,460
+بpreparation عادى زى أي ستان مش زى الجنس الستان
+
+233
+00:17:57,460 --> 00:18:00,460
+باختلف لأ الanthonas method هعمل السمير
+
+234
+00:18:00,460 --> 00:18:04,760
+بpreparation عادى هعمل air-drying مش هعمل heat
+
+235
+00:18:04,760 --> 00:18:09,180
+-fixation وذكرنا الصباغة بالنسبة لأ الصباغة هستخدم
+
+236
+00:18:09,180 --> 00:18:13,820
+two reagents أول إشي ال primary stain هيكون ال
+
+237
+00:18:13,820 --> 00:18:17,680
+crystal violet only which gives the bacterial cell
+
+238
+00:18:17,680 --> 00:18:21,750
+and its capsular material a dark purpleبقولّي هى
+
+239
+00:18:21,750 --> 00:18:27,230
+تعطى لون البربول للكبسولر للبكتيريا السلوى
+
+240
+00:18:27,230 --> 00:18:31,090
+الكبسولر material unlike the cell انكمل the
+
+241
+00:18:31,090 --> 00:18:34,630
+capsule is non-ionic and the primary stain cannot
+
+242
+00:18:34,630 --> 00:18:39,110
+adhere بقولّي اتفقنا انه الكبسول non-ionic ماقله
+
+243
+00:18:39,110 --> 00:18:42,430
+شحنة بالتالي ال primary stain ال crystal violet مش
+
+244
+00:18:42,430 --> 00:18:50,470
+هتقدر تلتصق فيهمدت الصباغة في الـCrystal Violet في
+
+245
+00:18:50,470 --> 00:18:54,210
+خطوة الـAnthonys Method بقولّي من 4 لـ7 دقائق
+
+246
+00:18:54,210 --> 00:18:59,470
+بخليها مغمورة بالـCrystal Violet من 4 لـ6 دقائق في
+
+247
+00:18:59,470 --> 00:19:05,450
+الـGram Stain لما أنا صبغت البكتيريا
+
+248
+00:19:05,450 --> 00:19:09,590
+بالـGram Stain استخدمت الـPrimary Stain الـCrystal
+
+249
+00:19:09,590 --> 00:19:12,930
+Violet لكن أنا خلّت الـCrystal Violet one minute
+
+250
+00:19:13,340 --> 00:19:18,060
+لكن هان بقوللي انه انا لازم اخليها من اربع لسبع
+
+251
+00:19:18,060 --> 00:19:22,020
+دقائق بقوللي المدة الطويلة هاي بتكون as mordant
+
+252
+00:19:22,020 --> 00:19:26,220
+يبقى بعد خطوة ال primary stain في عندي mordant ال
+
+253
+00:19:26,220 --> 00:19:31,520
+mordant هان المدة الطويلة للصبغة هتثبت الصبغة داخل
+
+254
+00:19:31,520 --> 00:19:38,930
+البكتيريا بعد ما اناأخلص أتخلص أعمل اللي هو أتخلص
+
+255
+00:19:38,930 --> 00:19:43,150
+الأربع لسبعة دقائق هل هغسل؟ بقول لي مش هتغسلي لأنه
+
+256
+00:19:43,150 --> 00:19:47,150
+احنا قولنا انه الـ Capsular material water soluble
+
+257
+00:19:47,150 --> 00:19:51,510
+فبالتالي ممكن الكابسول هينزل خلال عملية ال washing
+
+258
+00:19:51,510 --> 00:19:56,340
+هيعطيني false negative resultهعمل خطوة الـ
+
+259
+00:19:56,340 --> 00:19:59,720
+Decolorizer الـ Decolorizer بقولّي اللي هو الـ
+
+260
+00:19:59,720 --> 00:20:04,220
+cover sulphate solution 20% نسبته cover sulphate
+
+261
+00:20:04,220 --> 00:20:08,420
+solution نحفظ التركيز في الـ Indus Bar ماري معناه
+
+262
+00:20:08,420 --> 00:20:12,120
+الـ cover salt هي كانت من مكونات الملقاقات الـ
+
+263
+00:20:12,120 --> 00:20:14,820
+green اللي كانت تعتبر water soluble عشان ما انا
+
+264
+00:20:14,820 --> 00:20:18,140
+خربتش هان cover sulphate اللي هو يعتبر الـ
+
+265
+00:20:18,140 --> 00:20:21,620
+Decolorizer في خطوة الـ Anthonys method لـ
+
+266
+00:20:21,620 --> 00:20:25,890
+capsular stampبقولّي هذه المادة الكيميائية الـ
+
+267
+00:20:25,890 --> 00:20:30,830
+copper sulfate بتعمل دور مزدوج Dual roles as both
+
+268
+00:20:30,830 --> 00:20:35,130
+the decolorizing agent and counterstain يبقى
+
+269
+00:20:35,130 --> 00:20:38,950
+الـprimer stain الـcrystal violet الماردنت المدة
+
+270
+00:20:38,950 --> 00:20:44,110
+الطويلة لصبغة من 4 إلى 7 دقائق الـdecolorizer و
+
+271
+00:20:44,110 --> 00:20:49,350
+الـcounterstain هيكون الـ20% copper sulfate طيب،
+
+272
+00:20:49,350 --> 00:20:55,130
+شو هي الوظيفة لـ الـ copper sulfate؟بقول لي إنه
+
+273
+00:20:55,130 --> 00:20:59,030
+يعتبر كـ decolorizer أو ممكن أنا أعتبره washer
+
+274
+00:20:59,030 --> 00:21:01,930
+كمان لأنه مش هستخدم الميه لأنه أحنا قلنا الـ
+
+275
+00:21:01,930 --> 00:21:06,190
+capsular material هي تعتبر water soluble فهيعتبر
+
+276
+00:21:06,190 --> 00:21:10,970
+كمان washer it removes excess primary stain بقول
+
+277
+00:21:10,970 --> 00:21:15,610
+لي هيزيل أي إضافات من ال primary stain إحنا بعد أي
+
+278
+00:21:15,610 --> 00:21:19,050
+صبغة بنغسل عشان أي إش زيادة يروحها مش هستخدم ال
+
+279
+00:21:19,050 --> 00:21:24,080
+water فهو هيغسل كمان يبقى هيستخدم ك washeras well
+
+280
+00:21:24,080 --> 00:21:29,380
+as color from the capsule only and capsule appear
+
+281
+00:21:29,380 --> 00:21:32,600
+faint light blue يبقى خلّينا في هاي كمان بقولك
+
+282
+00:21:32,600 --> 00:21:35,960
+هيزيل اللون من الكابسول اشهر طبيعي اللون هيروح من
+
+283
+00:21:35,960 --> 00:21:41,480
+الكابسول لأن الكابسول مايله شحنة non-ionic طيب هاي
+
+284
+00:21:41,480 --> 00:21:46,040
+وظيفته ك decolorizer أو washer بقولي and capsule
+
+285
+00:21:46,040 --> 00:21:50,340
+appear light blue طيب كيف الكابسول هياخد اللون
+
+286
+00:21:50,340 --> 00:21:55,030
+الأزرق الفاتح وهو مايله شحنةومرتب تطفي الصبغة
+
+287
+00:21:55,030 --> 00:22:01,870
+قولنا الكابر سلفات هيشتغل ك counter stand كيف
+
+288
+00:22:01,870 --> 00:22:07,670
+هيشتغل الكابر سلفات ك counter stand ويعطي الكابسول
+
+289
+00:22:07,670 --> 00:22:11,390
+اللون الأزرق بالرغم من أنه ما إله شحنة بقولي
+
+290
+00:22:11,390 --> 00:22:15,470
+الكابر سلفات هيشتغل ك counter stand من خلال أنه
+
+291
+00:22:15,470 --> 00:22:20,200
+هيعملي فرق في الأيونات يبقىهذا الفرق هيعطي
+
+292
+00:22:20,200 --> 00:22:24,820
+الكابسول بعض من الأيونات يعني هيعطي بعض من الشحنات
+
+293
+00:22:24,820 --> 00:22:29,960
+بالتالي الصبغة هترتبط فيه وهتنطق .. هتطلع خارج
+
+294
+00:22:29,960 --> 00:22:34,600
+الخلية وهيسير للكابسول هتعطيه لون أزرق فأفرح يبقى
+
+295
+00:22:34,600 --> 00:22:40,740
+هيك اشتغل ال .. ال cover solvent كCounter Stain
+
+296
+00:22:40,740 --> 00:22:44,460
+انه اعطى الكابسول اللون ال light blue اشتغل ك
+
+297
+00:22:44,460 --> 00:22:47,920
+decolorizing agent من خلال انه زال اللون من
+
+298
+00:22:47,920 --> 00:22:53,360
+الكابسول يبقى ال counter الكبرسالفات اشتغلي ك
+
+299
+00:22:53,360 --> 00:22:56,540
+counter stain مش يعني counter stain يعني ميزلي
+
+300
+00:22:56,540 --> 00:22:59,900
+حاجة عن حاجة الخلية البكتيرية اعطاها اللون ال
+
+301
+00:22:59,900 --> 00:23:04,100
+purple بشكل طبيعي لكن الكابسول اعطاها اللون الفاتح
+
+302
+00:23:04,100 --> 00:23:09,030
+ال light blue يبقى هيك كان ك counter stainبالنسبة
+
+303
+00:23:09,030 --> 00:23:15,050
+للخطوات أول خطوة قلنا aseptically transfer a loop
+
+304
+00:23:15,050 --> 00:23:18,410
+full of culture within a nicolating loop to the
+
+305
+00:23:18,410 --> 00:23:22,390
+saliva بدي أعمل smear preparation aseptically يعني
+
+306
+00:23:22,390 --> 00:23:26,610
+بدي أمنع أي شيء كنت مانعش أن أستخدم استيرال loop
+
+307
+00:23:26,610 --> 00:23:32,150
+أشتغل في مكان معقّم لو استخدمت مثلا البكتيريا من
+
+308
+00:23:32,150 --> 00:23:36,870
+إبلات تكون ال normal saline استيرال كل هايبسيبتك
+
+309
+00:23:36,870 --> 00:23:40,290
+تكنيك عشان امنع الcontamination الخطوة التانية
+
+310
+00:23:40,290 --> 00:23:45,050
+هعمل air drying بعدها مش هعمل heat fixation لان
+
+311
+00:23:45,050 --> 00:23:52,270
+اتفقنا ان هو fragile ايش ال fragile يعني الكابسول
+
+312
+00:23:52,270 --> 00:23:56,490
+ممكن انه يتكسر بالحرارة فبالتالي يعطيني false
+
+313
+00:23:56,490 --> 00:24:02,690
+negative result في سبب تاني انهليش أنا بديش اعمل
+
+314
+00:24:02,690 --> 00:24:06,950
+heat fixation في ال slide الأبل in this procedure
+
+315
+00:24:06,950 --> 00:24:11,410
+السمير shouldn't be hit heat fixed since shrinkage
+
+316
+00:24:11,410 --> 00:24:15,210
+is likely to occur and create a clear zone around
+
+317
+00:24:15,210 --> 00:24:19,070
+the bacteria which can be mistaken for a capsule
+
+318
+00:24:19,070 --> 00:24:24,110
+بقوللي انا لو انا روحت عملت heat fixation ممكن
+
+319
+00:24:24,110 --> 00:24:28,590
+الخلية البكتيرية يصير لها shrinkingلما تنكمش
+
+320
+00:24:28,590 --> 00:24:32,190
+الخلية البكتيرية هيكون في مكان فاضي create clear
+
+321
+00:24:32,190 --> 00:24:36,790
+zone around the bacterium فممكن أنا أفكر ال clear
+
+322
+00:24:36,790 --> 00:24:40,970
+zone هذا هو الكابسول لكن مش كابسول هيعطيني false
+
+323
+00:24:40,970 --> 00:24:44,810
+positive result يبقى في عندي سببين لعدم عمل خطوة
+
+324
+00:24:44,810 --> 00:24:48,650
+ال heat fixation السبب الأول لأنه fragile الكابسول
+
+325
+00:24:48,650 --> 00:24:51,970
+فبالتالي ممكن يتكسر بالحرارة ويعطيني false
+
+326
+00:24:51,970 --> 00:24:56,360
+negative result لكن السبب التانيممكن البكتيريا
+
+327
+00:24:56,360 --> 00:24:59,560
+يصير لها shrinking فبالتالي تكون الـ clear zone
+
+328
+00:24:59,560 --> 00:25:04,160
+أفكره هو الكابسول فهي يعطيني false positive result
+
+329
+00:25:04,160 --> 00:25:10,120
+نكمل الخطوات الخطوة اللي بعدها أعمل staining بالـ
+
+330
+00:25:10,120 --> 00:25:12,780
+Primer Stain اللي هي الـ crystal violet يبقى ال
+
+331
+00:25:12,780 --> 00:25:16,340
+crystal violet هي الـ Primer Stain هخليها من أربع
+
+332
+00:25:16,340 --> 00:25:20,000
+لسبع دقائق وقولنا هي الأربع لسبع دقائق هتكون
+
+333
+00:25:20,000 --> 00:25:25,270
+الموردنت عشرين في الميةالخطوة التي سأقوم بها هي كـ
+
+334
+00:25:25,270 --> 00:25:29,270
+Decalarizer أو Washer وهي نفس الشيء كـ Counter
+
+335
+00:25:29,270 --> 00:25:32,970
+Stain إذا ملاحظين ما كان في خطوة اللي هو هغسل
+
+336
+00:25:32,970 --> 00:25:36,090
+بالميه لأن احنا قلنا الـ Capsular Material هي
+
+337
+00:25:36,090 --> 00:25:40,990
+تعتبر Water Soluble هعمل Air Drying و هشوفها تحت
+
+338
+00:25:40,990 --> 00:25:45,970
+الميكروسكوب على الـ Oil Emergent يبقى نكتب
+
+339
+00:25:45,970 --> 00:25:49,830
+الـCrystal
+
+340
+00:25:49,830 --> 00:25:50,490
+Violet
+
+341
+00:25:54,140 --> 00:25:57,560
+الاربع عال سبعة هتكون مواردانت والعشرين في المية
+
+342
+00:25:57,560 --> 00:26:04,980
+هيكون الكبر سلفات هيكون decalarizer and
+
+343
+00:26:04,980 --> 00:26:07,300
+counterstain
+
+344
+00:27:30,620 --> 00:27:34,540
+يبقى في هاي طريقة الانثوناز method تعرفنا على ال
+
+345
+00:27:34,540 --> 00:27:37,960
+primary stain الموردانت ال decolorizer and ال
+
+346
+00:27:37,960 --> 00:27:41,660
+counter stain في طريقة ال genes method ما استخدمت
+
+347
+00:27:41,660 --> 00:27:44,520
+ولا primary ولا موردانت ولا decolorizer أو counter
+
+348
+00:27:44,520 --> 00:27:48,660
+stain كانتالفكرة إنه أنا بدأ أستخدم أسدكستان كانت
+
+349
+00:27:48,660 --> 00:28:05,300
+أوبازاكستان فبالتالي هيبين عندي ال capsule هذه
+
+350
+00:28:05,300 --> 00:28:11,140
+الصورة بتوضح بعض الخطوات في الصورة قلنا crystal
+
+351
+00:28:11,140 --> 00:28:14,160
+violet primary stain الأربع على سبعة minutes هتكون
+
+352
+00:28:14,160 --> 00:28:19,030
+more thanبعدها مش هغسل بالميه هعمل اللى هغسل بيه
+
+353
+00:28:19,030 --> 00:28:22,030
+ال cover salivate عشرين في الميه cover salivate
+
+354
+00:28:22,030 --> 00:28:27,030
+هتكون كخطوط ال decalarizer و ال counter stand هذا
+
+355
+00:28:27,030 --> 00:28:31,310
+بالنسبة لنتائج اللى هى ال capsular stand باستخدام
+
+356
+00:28:31,310 --> 00:28:35,950
+طريقة ال anthonis method ال capsule منلاحظ أن هو
+
+357
+00:28:36,540 --> 00:28:40,540
+البكتيريا ماخدة اللون الـ dark purple عادي لأنه
+
+358
+00:28:40,540 --> 00:28:43,660
+هتاخد الـ crystal violet بشكل طبيعي لأنه هي basic
+
+359
+00:28:43,660 --> 00:28:48,160
+stain هتصبغ البكتيريا الصالبة الكابسول هياخد لون
+
+360
+00:28:48,160 --> 00:28:52,000
+فاتح كتير لون موف فاتح او ممكن نعتبره أزرق فاتح
+
+361
+00:28:52,000 --> 00:28:55,520
+لأنه احنا قلنا الكبر سلفات هيعملي فرق في الأيونات
+
+362
+00:28:55,520 --> 00:28:59,340
+هيعطي الكابسول ايونات يعني هيصيرله شحنة فبالتالي
+
+363
+00:28:59,340 --> 00:29:05,080
+هتطلع جزء من الصبغة لخرج البكتيريا وهتتلون باللون
+
+364
+00:29:05,430 --> 00:29:09,450
+الـ Light Blue أو الـ Light Purple بدنا نفرق
+
+365
+00:29:09,450 --> 00:29:13,070
+بنتيجة الـ Anthony's method والـ Gen's method ال
+
+366
+00:29:13,070 --> 00:29:16,790
+result بتختلف حسب الطريقة بدنا نكون عارفين كل
+
+367
+00:29:16,790 --> 00:29:22,010
+طريقة شو ال principle تبعها ايش ال procedure لكل
+
+368
+00:29:22,010 --> 00:29:27,250
+طريقة وكيف بتكون النتيجة في كل طريقة هذا بالنسبة
+
+369
+00:29:27,250 --> 00:29:28,970
+لـ Capsular Style
+
diff --git a/PL9fwy3NUQKwZWgAb_V1ATlRRsH-6i3cPC/YWBUtjE9yHc_raw.srt b/PL9fwy3NUQKwZWgAb_V1ATlRRsH-6i3cPC/YWBUtjE9yHc_raw.srt
new file mode 100644
index 0000000000000000000000000000000000000000..96915543ac506cb19942c7293ecb001c9f82ea5f
--- /dev/null
+++ b/PL9fwy3NUQKwZWgAb_V1ATlRRsH-6i3cPC/YWBUtjE9yHc_raw.srt
@@ -0,0 +1,2364 @@
+1
+00:00:02,090 --> 00:00:05,750
+السلام عليكم ورحمة الله وبركاته يعطيكم العافية
+
+2
+00:00:05,750 --> 00:00:09,190
+جميعا في هذا اللقاء نستكمل سلسلة ال biochemical
+
+3
+00:00:09,190 --> 00:00:12,910
+test في المحاضرة السابقة اتكلمنا على الانزيمات
+
+4
+00:00:12,910 --> 00:00:16,090
+وقسمنا الانزيم حسب ال side of production ل
+
+5
+00:00:16,090 --> 00:00:19,930
+endoenzyme و exoenzyme ال endoenzyme قلنا انه
+
+6
+00:00:19,930 --> 00:00:24,670
+التفاعل بيصير intracellular داخل الخليةلكن الـ
+
+7
+00:00:24,670 --> 00:00:28,590
+Exoenzyme التفاعل بيصير Extracellular خارج الخلية
+
+8
+00:00:28,590 --> 00:00:33,770
+في ال Exoenzyme الإنزيم يفرز داخل الخلية لكن بيطلع
+
+9
+00:00:33,770 --> 00:00:37,150
+بر الخلية لأن ال substrate اللي بيشتغل عليها ال
+
+10
+00:00:37,150 --> 00:00:42,670
+Exoenzyme حجمها كبير high molecular weight يبقى ال
+
+11
+00:00:42,670 --> 00:00:45,490
+substrate اللي هيشتغل عليها ال Exoenzyme high
+
+12
+00:00:45,490 --> 00:00:49,430
+molecular weight such as polysaccharide lipid and
+
+13
+00:00:49,430 --> 00:00:53,850
+proteinهدول يعتبروا macromolecules ما بيقدروا
+
+14
+00:00:53,850 --> 00:00:57,970
+يدخلوا جوا الخلية عشان هيك لازم يطلع الانزيم لبرا
+
+15
+00:00:57,970 --> 00:01:01,870
+ويصير التفاعل extracellular من الأمثل على
+
+16
+00:01:01,870 --> 00:01:04,970
+البوليساكرايد ال starch من الأمثل على الليبد ال
+
+17
+00:01:04,970 --> 00:01:09,550
+triglyceride من الأمثل على ال protein fibrinogen
+
+18
+00:01:09,550 --> 00:01:14,830
+casein gelatin الamylase انزيم بيشتغل على ال
+
+19
+00:01:14,830 --> 00:01:19,070
+starchالـ lipase بيشتغل على الـ triglyceride الـ
+
+20
+00:01:19,070 --> 00:01:22,170
+coagulase أخدناها في المحاضرة السابقة قولنا هو
+
+21
+00:01:22,170 --> 00:01:28,670
+إيجزو أنزايم بيشتغل على الفيبرنوجين الكازيناز
+
+22
+00:01:28,670 --> 00:01:32,310
+قولنا بيشتغل على الكازين الجيلاتيناز بيشتغل على
+
+23
+00:01:32,310 --> 00:01:37,270
+الجيلاتينهدول كلهم يعتبروا exo-enzyme لأن ال
+
+24
+00:01:37,270 --> 00:01:42,610
+substrate اللي بيشتغله عليها تعتبر high molecular
+
+25
+00:01:42,610 --> 00:01:46,670
+weight حجمها كبير ما بتقدر تدخل لداخل الخلية عشان
+
+26
+00:01:46,670 --> 00:01:51,990
+هي ال enzymات بتفرس داخل الخلية و تطلع لبرا عشان
+
+27
+00:01:51,990 --> 00:01:57,470
+يصير التفاعل extra cellular لو رجعنا لسلايدة type
+
+28
+00:01:57,470 --> 00:02:01,790
+of enzyme حسب ال site of productionفي الـ
+
+29
+00:02:01,790 --> 00:02:07,130
+Exoenzyme كان ذاكر أمثلة هنلاقي هذه الانزيمات هي
+
+30
+00:02:07,130 --> 00:02:13,350
+مذكورة في الجملة يبقى عشان نحفظ الـ Exoenzyme بشوف
+
+31
+00:02:13,350 --> 00:02:17,590
+شو ال substrate اللي بيشتغل عليها ال enzyme إذا ال
+
+32
+00:02:17,590 --> 00:02:22,510
+substrate polysaccharide or lipid or protein يبقى
+
+33
+00:02:22,510 --> 00:02:25,750
+هاي تعتبر high molecular weight يبقى ال enzyme
+
+34
+00:02:25,750 --> 00:02:30,160
+يعتبر Exoenzymeاليوم ان شاء الله في هذه المحاضرة
+
+35
+00:02:30,160 --> 00:02:35,580
+هنتكلم عن الاميلاز and جيلاتيناز هنبدأ بالاميلاز
+
+36
+00:02:35,580 --> 00:02:40,340
+production or starch hydrolysis test أول معلومة
+
+37
+00:02:40,340 --> 00:02:45,600
+عرفناها عن الاميلازإنه Exoenzyme السبسترات اللي
+
+38
+00:02:45,600 --> 00:02:49,680
+بيشتغل عليها ال amylase هي ال starch ال starch
+
+39
+00:02:49,680 --> 00:02:54,940
+يعتبر polysaccharide ال amylase بيعمل hydrolysis ل
+
+40
+00:02:54,940 --> 00:02:59,560
+ال starch عشان هي اسم الفحص starch hydrolysis test
+
+41
+00:02:59,560 --> 00:03:03,540
+or amylase production لأنه بيعمل hydrolysis لل
+
+42
+00:03:03,540 --> 00:03:07,600
+starch ال starch عبارة عن its chain of
+
+43
+00:03:07,600 --> 00:03:12,150
+monosaccharideمن الـ monosaccharide الجليكوز لو
+
+44
+00:03:12,150 --> 00:03:16,730
+بدنا نتعرف على الـ type of sugar الـ types of
+
+45
+00:03:16,730 --> 00:03:20,410
+sugar فيه عندى monosaccharide, disaccharide و
+
+46
+00:03:20,410 --> 00:03:25,370
+polysaccharide الـ monosaccharide جليكوز galactose
+
+47
+00:03:25,370 --> 00:03:29,050
+افراكتوز هي أمثلة على monosaccharide الـ
+
+48
+00:03:29,050 --> 00:03:33,150
+disaccharide بتتكون من two monosaccharide مرتبطين
+
+49
+00:03:33,150 --> 00:03:37,900
+من الأمثلة على الـ disaccharideSaccharose,
+
+50
+00:03:38,020 --> 00:03:41,680
+Maltose, Lactose الـ Saccharose عبارة عن جليكوز
+
+51
+00:03:41,680 --> 00:03:46,320
+and فركتوز المالتوز عبارة عن جليكوز زائد جليكوز
+
+52
+00:03:46,320 --> 00:03:51,200
+اللاكتوز جليكوز زائد جلاكتوز تالت نوع الـ
+
+53
+00:03:51,200 --> 00:03:54,720
+Polysaccharide مثال عليها الـ starch قولنا أن الـ
+
+54
+00:03:54,720 --> 00:03:59,560
+starch عبارة عن polymer of مونو ساكرايد من المونو
+
+55
+00:03:59,560 --> 00:04:03,080
+ساكرايد الجليكوز من الأمثلة الأخرى على ال
+
+56
+00:04:03,080 --> 00:04:06,620
+polysaccharide في عندي الجليكوجين وعندي السليلوز
+
+57
+00:04:07,200 --> 00:04:11,360
+لكن اليوم ان شاء الله هنتكلم احنا عن ال amylase
+
+58
+00:04:11,360 --> 00:04:15,420
+اللى بيشتغل على ال starch ال starch يعتبر
+
+59
+00:04:15,420 --> 00:04:20,140
+polysaccharide بتكون من polymer of glycose ال
+
+60
+00:04:20,140 --> 00:04:24,380
+starch هيدروليزز تكسير ال starch بيصير بواسط ال
+
+61
+00:04:24,380 --> 00:04:29,400
+two type of enzyme بواسط اتنوعين من الانزيمات اول
+
+62
+00:04:29,400 --> 00:04:35,060
+انزيم Alpha amylase Alpha وليس beta لأن ال betaيله
+
+63
+00:04:35,060 --> 00:04:38,420
+علاقة بالـ cellulose الـ cellulose الموجود في
+
+64
+00:04:38,420 --> 00:04:44,980
+البلاند الـ alpha amylase بيعمل hydrolysis of α1,4
+
+65
+00:04:44,980 --> 00:04:49,880
+-glycosidic bond بيكسر الرابطة اللي اسمها α1,4
+
+66
+00:04:49,880 --> 00:04:54,520
+-glycosidic bond الانزيم التاني المسؤول عن تكسير
+
+67
+00:04:54,520 --> 00:04:59,880
+ال starch Oligo-1,6-glycosidase مسؤول عن تكسير
+
+68
+00:04:59,880 --> 00:05:05,920
+الرابطة α1,6-glycosidic bondعشان نوضح الصورة اكتر
+
+69
+00:05:05,920 --> 00:05:09,000
+هذه
+
+70
+00:05:09,000 --> 00:05:14,820
+الصورة بتوضح الـ starch structure الـ starch قولنا
+
+71
+00:05:14,820 --> 00:05:18,240
+عبارة عن its chain of monosaccharide الـ
+
+72
+00:05:18,240 --> 00:05:21,360
+monosaccharide اللي هو الجليكوز يبقى هو عبارة عن
+
+73
+00:05:21,360 --> 00:05:25,760
+سلسلة من الجليكوز هاي الوحدات الخضراء عبارة عن
+
+74
+00:05:25,760 --> 00:05:32,640
+الجليكوزإذا كان ال ال structure chain الرابط ما
+
+75
+00:05:32,640 --> 00:05:36,900
+بينهم بتكون alpha one four linkage لو كان في عندي
+
+76
+00:05:36,900 --> 00:05:41,620
+branch بتكون الرابط alpha one six linkage احنا
+
+77
+00:05:41,620 --> 00:05:46,900
+قلنا انه في عندي two type two enzyme مسئولين عن ال
+
+78
+00:05:46,900 --> 00:05:51,020
+starch hydrolysis الانزيم الأول اللي هو ال alpha
+
+79
+00:05:51,020 --> 00:05:54,720
+amylase ال alpha amylase قلنا مسئول عن تكسير ال
+
+80
+00:05:55,030 --> 00:05:59,410
+الرابطة الهيدروليسيز للرابط ألفة وان فار في
+
+81
+00:05:59,410 --> 00:06:03,550
+جلايكوسيدكبون يبقى ال alpha amylase هيجي هنا في
+
+82
+00:06:03,550 --> 00:06:08,030
+حال كان في عندي chain في عندي سلسلة هيجي يكسر هاي
+
+83
+00:06:08,030 --> 00:06:12,950
+الروابط ويعطيني اما لو كان اعطا لو ايجي هنا من هاي
+
+84
+00:06:12,950 --> 00:06:17,530
+الرابطة لو
+
+85
+00:06:17,530 --> 00:06:23,430
+كسر هنا اتنين جليكوز أداني مالتوز لو كسر هناهيديني
+
+86
+00:06:23,430 --> 00:06:29,950
+تلاتة جيليكوز بسميهم malto triose تمام لو اتكسرت
+
+87
+00:06:29,950 --> 00:06:33,630
+هان الرابط هذا المركب اذا ضال ال branch بسميه
+
+88
+00:06:33,630 --> 00:06:39,030
+dextrine هيجي ال alpha او oligo alpha 16
+
+89
+00:06:39,030 --> 00:06:43,670
+glycosidase هيكسر الرابط اللي هان اللي هي alpha 16
+
+90
+00:06:43,670 --> 00:06:49,170
+linkage ويديني maltose and maltotriose هذه كلها
+
+91
+00:06:49,170 --> 00:06:55,120
+المركباتعبارة عن law molecular weight soluble in
+
+92
+00:06:55,120 --> 00:06:58,980
+water بتقدر تدخل للخلية عبر ال cell membrane
+
+93
+00:06:58,980 --> 00:07:03,600
+تستخدم في ال energy production خلال عملية ال
+
+94
+00:07:03,600 --> 00:07:08,140
+glycolysis كمان تستخدم ك carbon source ال carbon
+
+95
+00:07:08,140 --> 00:07:11,580
+source طبعا عندي أنا ال carbohydrate هي من
+
+96
+00:07:11,580 --> 00:07:18,060
+الفائدتها اللي هي تعتبر كمصدر للكربون اللازم لنمو
+
+97
+00:07:18,060 --> 00:07:21,530
+البكتيريايبقى هاي كلها تعتبر اللي هو molecular
+
+98
+00:07:21,530 --> 00:07:26,450
+weight بتقدر تدخل للخلية الخلية بتقدر تستفيد منها
+
+99
+00:07:26,450 --> 00:07:31,470
+ممكن كمصدر للطاقة أو ممكن كمصدر للكربون اللازم
+
+100
+00:07:31,470 --> 00:07:39,070
+لنمو البكتيري نرجع
+
+101
+00:07:39,070 --> 00:07:45,020
+لأول slideStarch agar is a differential medium لما
+
+102
+00:07:45,020 --> 00:07:49,980
+قسمنا ال media حسب ال function قلنا انه في عندي
+
+103
+00:07:49,980 --> 00:07:53,460
+differential media ال differential media بتعطي كل
+
+104
+00:07:53,460 --> 00:07:58,180
+بكتيريا لون بالتالي بتميز بكتيريا عن بكتيريا وهذا
+
+105
+00:07:58,180 --> 00:08:01,940
+ان شاء الله هنشوفه في ال result that test the
+
+106
+00:08:01,940 --> 00:08:05,140
+ability of an organism to produce certain
+
+107
+00:08:05,140 --> 00:08:09,640
+exoenzyme ولي هذا الفحص بيكشف عن قدرة ال organism
+
+108
+00:08:10,200 --> 00:08:14,500
+إنه ينتج Certain Exoenzyme قولنا إن الـ Amylase
+
+109
+00:08:14,500 --> 00:08:19,220
+يعتبر Exoenzyme الإنزيمات المسؤولة عن الـ starch
+
+110
+00:08:19,220 --> 00:08:23,020
+Hydrolysis أول إنزيم اللي هو الـ Alpha Amylase
+
+111
+00:08:23,020 --> 00:08:27,120
+المسؤول عن تكسير الرابط Alpha-1-Pharyglycosidic
+
+112
+00:08:27,120 --> 00:08:30,840
+bond الإنزيم التاني يبقى هذا أول إنزيم اللي هو الـ
+
+113
+00:08:30,840 --> 00:08:31,840
+Alpha Amylase
+
+114
+00:08:35,090 --> 00:08:39,810
+الانزيم التاني اللي هو Oligo-1,6-Glycosidase
+
+115
+00:08:39,810 --> 00:08:45,570
+المسؤول عن تكسير الرابطة Alpha-1,6-Glycosidic bond
+
+116
+00:08:45,570 --> 00:08:50,790
+that hydrolyze starch بيكسروا ال starch لمركبات
+
+117
+00:08:50,790 --> 00:08:56,130
+صغيرة الحجم بتقدر تدخل لخلية starch molecules are
+
+118
+00:08:56,130 --> 00:08:59,490
+too large to enter the bacterial cell قولنا إن ال
+
+119
+00:08:59,490 --> 00:09:03,670
+starch يعتبر polysaccharide حجمه كبير ما بيقدر
+
+120
+00:09:03,670 --> 00:09:08,710
+يدخل الخليةSo some bacteria some مش كل البكتيريا
+
+121
+00:09:08,710 --> 00:09:13,410
+بتفرز الانزيمات المسؤولة عن تكسير ال starch some
+
+122
+00:09:13,410 --> 00:09:18,110
+bacteria secrete exoenzyme اتفقنا انه عبارة عن
+
+123
+00:09:18,110 --> 00:09:22,550
+exoenzyme to degrade starch into subunit بيكسل ال
+
+124
+00:09:22,550 --> 00:09:25,870
+starch ل subunit زي ما اتعرفنا اما مالتوز مالتو
+
+125
+00:09:25,870 --> 00:09:30,550
+تريوز او ديسترين هاي ال subunitthat can then be
+
+126
+00:09:30,550 --> 00:09:33,810
+utilized by the organism بتقدر تدخل للخلية
+
+127
+00:09:33,810 --> 00:09:43,090
+والorganism يقدر انه يستهلكها ويستفيد منها هيك
+
+128
+00:09:43,090 --> 00:09:48,930
+احنا عرفنا كيف الانزيم بيكسر ال starch كيف شغل
+
+129
+00:09:48,930 --> 00:09:52,930
+الانزيم طب كيف بدنا نكشف عن هذا الانزيم وهل
+
+130
+00:09:52,930 --> 00:09:59,270
+البكتيريا قادرة انها تكسر ال starch او لأأحنا قلنا
+
+131
+00:09:59,270 --> 00:10:04,190
+إن أي أنزيم بيشتغل على substrates الأميلاز بيشتغل
+
+132
+00:10:04,190 --> 00:10:09,170
+على ال starch يبقى عشان أكشف على الأميلاز لازم
+
+133
+00:10:09,170 --> 00:10:14,670
+أزرع البكتيريا على ميديا فيها starch starch agar
+
+134
+00:10:14,670 --> 00:10:20,350
+is a simple nutrative media بقول هي عبارة عن media
+
+135
+00:10:20,350 --> 00:10:24,830
+simple with starch added يبقى هي عبارة عن nutrient
+
+136
+00:10:24,830 --> 00:10:29,730
+agar مضاف إليها ال starch as supplementالسترش هو
+
+137
+00:10:29,730 --> 00:10:32,890
+عبارة عن ال substrate اللي هيشتغل عليه الانزين
+
+138
+00:10:32,890 --> 00:10:36,930
+فبالتالي لازم يكون موجود في الميديا السترش أجار
+
+139
+00:10:36,930 --> 00:10:41,490
+ممكن تيجي جاهزة ببوتل وممكن أنا أحضرها كيف بحضرها
+
+140
+00:10:41,490 --> 00:10:47,170
+بجيب ال nutrient أجار و بضيف عليها استرش since no
+
+141
+00:10:47,170 --> 00:10:50,210
+color change occur in the media when organism
+
+142
+00:10:50,210 --> 00:10:54,550
+hydrolyzes starch we added we add iodine to the
+
+143
+00:10:54,550 --> 00:10:58,950
+blood after incubation iodine turns blue purpleor
+
+144
+00:10:58,950 --> 00:11:02,370
+black dependent on the concentration of iodine in
+
+145
+00:11:02,370 --> 00:11:06,370
+the presence of starch عشان نفهم شو قصده بهذه
+
+146
+00:11:06,370 --> 00:11:11,110
+الفقرة خلينا نشرح ال procedure هذه الفقرة هي ال
+
+147
+00:11:11,110 --> 00:11:15,990
+principle الفحص من هنا هي ال principle الفحص لكن
+
+148
+00:11:15,990 --> 00:11:18,470
+خلينا نحكي عن ال procedure
+
+149
+00:11:26,190 --> 00:11:30,630
+البروسيجر بقولي انه انا بحضر ال starch agar طبعا
+
+150
+00:11:30,630 --> 00:11:34,470
+زي خطوات تحضير ال media اللي اخدناها بعد ما احضر
+
+151
+00:11:34,470 --> 00:11:38,310
+ال starch agar بزرع البكتيريا اللي بدي افحص قدرتها
+
+152
+00:11:38,310 --> 00:11:42,590
+على انتاج هذا الانزين كيف طريقة الزراعة بزرع على
+
+153
+00:11:42,590 --> 00:11:47,170
+شكل خط يبقى بقولي extract the test organism across
+
+154
+00:11:47,170 --> 00:11:53,020
+a sample portion of the agar surface امن الزراعالـ
+
+155
+00:11:53,020 --> 00:11:56,000
+organism اللى انا بدي افحص قدرته على انتاج هذا
+
+156
+00:11:56,000 --> 00:12:01,440
+الانزيم بشكل خطب باخد الانيكولام بعد ما انا زرعت
+
+157
+00:12:01,440 --> 00:12:06,320
+طبعا بحط هذا الطبق اللى انا زرعته فى ال incubator
+
+158
+00:12:06,320 --> 00:12:14,020
+على درجة حرارة 37 لمدة 48 ساعة او 24 بعد ال
+
+159
+00:12:14,020 --> 00:12:19,480
+incubation طبعا هلاقي انا عندى نمو للبكتيريا زرعت
+
+160
+00:12:19,480 --> 00:12:23,460
+البكتيريا نمت البكتيرياإذا كان عندها انزيم
+
+161
+00:12:23,460 --> 00:12:27,180
+الاميلاز هتكون كسرت ال starch وإذا ما كان عندها
+
+162
+00:12:27,180 --> 00:12:31,640
+الانزيم مش هتكون كسرت ال starch طيب كيف بدي أكشف
+
+163
+00:12:31,640 --> 00:12:37,340
+عن هال إشي بروح أنا بضيف indicator أو مادة تتفاعل
+
+164
+00:12:37,340 --> 00:12:41,980
+معها ال starch وتعطي لون معين لو البكتيريا عندها
+
+165
+00:12:41,980 --> 00:12:46,420
+انزيم الاميلاز هتكسر ال starch يعني ال starch مش
+
+166
+00:12:46,420 --> 00:12:51,240
+موجود يعني مش هيصير تفاعل وما رح يعطي اللون المعين
+
+167
+00:12:51,890 --> 00:12:56,770
+لكن لو البكتيريا ما عندها ال enzyme ال starch ما
+
+168
+00:12:56,770 --> 00:13:01,050
+رح يتكسر هيظل موجود في ال media يعني هيصير التفاعل
+
+169
+00:13:01,050 --> 00:13:06,210
+يعطي لون معين هذا هو ال principle الفحص طيب شو هو
+
+170
+00:13:06,210 --> 00:13:10,510
+ال indicator اللي احنا بنضيفه بقولنا بتضيفي iodine
+
+171
+00:13:10,510 --> 00:13:14,470
+ال iodine أخدناها في ال gram stain نفسه اللي احنا
+
+172
+00:13:14,470 --> 00:13:18,880
+استخدمناه في ال gram stain بقولنا بعدالـ
+
+173
+00:13:18,880 --> 00:13:22,980
+Incubation لمدة 24 ساعة وبعد ما تنمو البكتيريا
+
+174
+00:13:22,980 --> 00:13:28,540
+بغمر الطبق بال Iodine بغمر كل الطبق بال Iodine
+
+175
+00:13:28,540 --> 00:13:33,980
+وبشوف نتيجة التفاعل Iodine will turn blue when it
+
+176
+00:13:33,980 --> 00:13:38,800
+reacts with starch بقوللي ال Iodine بتحول للون
+
+177
+00:13:38,800 --> 00:13:43,170
+الأزرق لو اتفاعل مع ال starchA clear zone will be
+
+178
+00:13:43,170 --> 00:13:47,290
+seen where starch has been digested لو السترش
+
+179
+00:13:47,290 --> 00:13:52,450
+اتكسر بقولي النتيجة بتكون clear zone يبقى اللون ال
+
+180
+00:13:52,450 --> 00:13:56,210
+blue لو كانت النتيجة negative لكن لو كانت النتيجة
+
+181
+00:13:56,210 --> 00:14:00,270
+positive موجود الأميلاز بتكون clear zone ال
+
+182
+00:14:00,270 --> 00:14:05,770
+principle الفحص لسترش لو كانت البكتيريا ما عندها
+
+183
+00:14:05,770 --> 00:14:11,740
+أنزيم الأميلازي بقى كانت negative للأميلازماتكسر
+
+184
+00:14:11,740 --> 00:14:16,720
+الاسترش دلو موجود ضفت الايوداين الايوداين اتفاعل
+
+185
+00:14:16,720 --> 00:14:20,420
+مع الاسترش واعطاني لون blue او purple او black
+
+186
+00:14:20,420 --> 00:14:23,940
+طبعا بيعطيني اللون اي لون من هذه الألوان حسب ال
+
+187
+00:14:23,940 --> 00:14:29,180
+concentration of الايوداين لو كانت البكتيريا عندها
+
+188
+00:14:29,180 --> 00:14:32,580
+انزيم الاميلازي كانت positive للاميلازي يبقى
+
+189
+00:14:32,580 --> 00:14:36,990
+الاسترش مش هيكون موجودلما أضيف ال iodine مش هيصير
+
+190
+00:14:36,990 --> 00:14:40,410
+في عندي لون فبالتالي بقولي هيكون في عندي clear
+
+191
+00:14:40,410 --> 00:14:48,470
+zone حوالين البكتيريا هذا بالنسبة لل principle هذا
+
+192
+00:14:48,470 --> 00:14:53,410
+الطبق بعد ما انا طلعته من ال incubator بعد ما نمت
+
+193
+00:14:53,410 --> 00:14:57,970
+البكتيريا هذا الطبق بعد ما انا ضفت ال iodine تمام
+
+194
+00:14:57,970 --> 00:15:02,960
+بقولي انهبعد ما اناضفت ال iodine في زراعة بكتيريا
+
+195
+00:15:02,960 --> 00:15:07,940
+X و بكتيريا Y نلاحظ انه بكتيريا X هانتكون ال clear
+
+196
+00:15:07,940 --> 00:15:12,420
+zone قولنا ال clear zone معناه انه ال starch سله
+
+197
+00:15:12,420 --> 00:15:16,440
+هيدروليسز اتكسر يبقى ال starch مش موجود لما ييجي
+
+198
+00:15:16,440 --> 00:15:20,060
+ال iodine يرتبط فيه مش هيكون فيه تفاعل فهيكون فيه
+
+199
+00:15:20,060 --> 00:15:25,760
+clear zone يبقى النتيجة هاي هي positive لكن هان
+
+200
+00:15:25,760 --> 00:15:31,210
+النتيجة هي negativeهنا الاسترش ما اتكسر الاسترش
+
+201
+00:15:31,210 --> 00:15:35,010
+دلو موجود ايجا ال iodine ارتبط معاه اعطاني لون
+
+202
+00:15:35,010 --> 00:15:40,210
+blue او purple او black حسب ال concentration of
+
+203
+00:15:40,210 --> 00:15:43,350
+iodine لكن احنا في المعمل ان شاء الله هيكون اللون
+
+204
+00:15:43,350 --> 00:15:48,230
+black بقولي من الأمثلة على ال amylase positive
+
+205
+00:15:48,230 --> 00:15:51,750
+اللي هي البصيلة ال subcules هي موجودة على الشمال
+
+206
+00:15:52,070 --> 00:15:55,990
+لكن الـ E.coli موجودة على اليمين البصيلة الـ
+
+207
+00:15:55,990 --> 00:15:59,290
+Subtules هي positive للأميلاز ملاحظ في عندي clear
+
+208
+00:15:59,290 --> 00:16:04,350
+zone around growth لكن هنا في عندي اللون اللي هو
+
+209
+00:16:04,350 --> 00:16:09,590
+مش واضح كتير لكن بيكون اللون black يبقى البصيلة
+
+210
+00:16:09,590 --> 00:16:13,770
+الـ Subtules مثال على الأميلاز positive لكن الـ E
+
+211
+00:16:13,770 --> 00:16:20,180
+.coli مثال على الأميلاز negativeأحنا قلنا في أول
+
+212
+00:16:20,180 --> 00:16:24,900
+ال .. أول slide أنه الـ starch agar تعتبر
+
+213
+00:16:24,900 --> 00:16:28,700
+differential media بتميز بكتيريا عن بكتيريا وهذا
+
+214
+00:16:28,700 --> 00:16:32,020
+اللي اللي أحنا لاحظناه في ال result ميازات لي
+
+215
+00:16:32,020 --> 00:16:37,560
+أعطتني في البصل لسه بتيوز لون clear zone لكن في ال
+
+216
+00:16:37,560 --> 00:16:41,500
+E.coli أعطتني لون تاني فقدرت أميز بكتيريا عن
+
+217
+00:16:41,500 --> 00:16:42,440
+بكتيريا
+
+218
+00:16:44,800 --> 00:16:48,480
+بالنسبة للأميلاز النتائج إذا كانت عندها positive
+
+219
+00:16:48,480 --> 00:16:51,620
+إذا كان عندها ال enzyme يبقى بيكون في عندى clear
+
+220
+00:16:51,620 --> 00:16:55,420
+zone of hydrolysis مثال عليها البصلة ال subcules
+
+221
+00:16:55,420 --> 00:16:59,800
+لكن لو كانت negative للأميلاز بيكون اللون blue
+
+222
+00:16:59,800 --> 00:17:03,300
+purple or black حسب ال concentration of iodine
+
+223
+00:17:03,300 --> 00:17:07,200
+مثال عليها ال E coli هذه الصورة هي في عندى clear
+
+224
+00:17:07,200 --> 00:17:11,920
+zone حوالين النمو يبقى هيالنتيجة positive هاي بصل
+
+225
+00:17:11,920 --> 00:17:16,720
+ل subcules لكن هان بلاحظ ان في عندي اللون أسود
+
+226
+00:17:16,720 --> 00:17:22,280
+حوالين البكتيريا أو حوالين النمو يبقى هي ال E.coli
+
+227
+00:17:22,280 --> 00:17:27,700
+طبعا بلاحظ هان ان هاي المناطق ماوصلت إليها
+
+228
+00:17:27,700 --> 00:17:32,780
+البكتيريا فبالتالي هيظل ال starch موجود هيرتبط بال
+
+229
+00:17:32,780 --> 00:17:34,680
+أيضاين ويعطيين اللون ال black
+
+230
+00:17:38,890 --> 00:17:43,370
+في الـ slide اللي قبل كان ذاكر أنه البصيلة الـ sub
+
+231
+00:17:43,370 --> 00:17:47,230
+-tools البصيلة
+
+232
+00:17:47,230 --> 00:17:50,470
+الـ sub-tools هي positive للبحث و للفحص و الـ
+
+233
+00:17:50,470 --> 00:17:54,990
+Equality negative للفحص لكن ما بحكي أن هذا يعتبر
+
+234
+00:17:54,990 --> 00:17:59,460
+significantهذا مش significant فقط لكن مثال على الـ
+
+235
+00:17:59,460 --> 00:18:02,740
+positive و الـ negative للفحص لأن الـ E.coli جرام
+
+236
+00:18:02,740 --> 00:18:06,100
+نيجاتيف بصيلاي و البصيلة السابتيولز تعتبر جرام
+
+237
+00:18:06,100 --> 00:18:09,480
+positive بصيلاي يبقى من الأصل من الجرامستين بقدر
+
+238
+00:18:09,480 --> 00:18:13,500
+أني أنا أفرق بينهم غير كده أن الـ E.coli تعتبر non
+
+239
+00:18:13,500 --> 00:18:17,360
+spar farming البصيلة السابتيولز تعتبر spar farming
+
+240
+00:18:17,360 --> 00:18:22,600
+لو بدنا نفرق الاميلاز positive للبصيلة السابتيولز
+
+241
+00:18:22,600 --> 00:18:28,010
+و ال E.coli اللي هي الاميلاز نيجاتيفهك منكون خلصنا
+
+242
+00:18:28,010 --> 00:18:33,570
+أول فحص اللى هو الـomyelase هنتقل للجيلاتيناز
+
+243
+00:18:33,570 --> 00:18:40,910
+هنتكلم
+
+244
+00:18:40,910 --> 00:18:44,830
+عن تانى أنزايم اللى هو الجيلاتيناز الجيلاتيناز
+
+245
+00:18:44,830 --> 00:18:48,990
+يعتبر exo enzyme ال substrate اللى هيشتغل عليها
+
+246
+00:18:48,990 --> 00:18:53,920
+الجيلاتينقلنا ان الجيلاتين يعتبر protein high
+
+247
+00:18:53,920 --> 00:18:58,600
+molecular weight لازم الانزيم يطلع لبرا ويكسره ل
+
+248
+00:18:58,600 --> 00:19:03,320
+low molecular weight هيكسره ل amino acid ال amino
+
+249
+00:19:03,320 --> 00:19:06,740
+acid هي سوليوبول بتقدر تدخل لل cell membrane
+
+250
+00:19:07,210 --> 00:19:12,050
+الأمينو أسد تعتبر كمصدر للنيتروجين نيتروجين source
+
+251
+00:19:12,050 --> 00:19:15,790
+تستخدم في ال synthesis of structural and
+
+252
+00:19:15,790 --> 00:19:20,950
+functional cellular protein اللازم لنمو البكتيريا
+
+253
+00:19:20,950 --> 00:19:24,050
+الجيلاتين
+
+254
+00:19:24,050 --> 00:19:28,550
+يعتبر بروتين مشتق من الكلجن الموجود في ال
+
+255
+00:19:28,550 --> 00:19:34,010
+connective tissue كانوا زمان يعتبروا الجيلاتينكـ
+
+256
+00:19:34,010 --> 00:19:38,590
+solidifying agent لكن في أنواع من البكتيريا بتفرز
+
+257
+00:19:38,590 --> 00:19:43,450
+أنزيم الجيلاتيناز بيكسر الجيلاتين وبتحول الوسط من
+
+258
+00:19:43,450 --> 00:19:49,630
+solid ل liquid وهي فكرة الكشف عن الانزيم ومعرفة هل
+
+259
+00:19:49,630 --> 00:19:54,210
+البكتيريا عندها انزيم الجيلاتيناز او لأ الفكرة انه
+
+260
+00:19:54,210 --> 00:19:59,010
+بيكون عندى في ال medium جيلاتين كsubstrate
+
+261
+00:19:59,710 --> 00:20:03,590
+الجيلاتينياز طبعا في البكتيريا هاكشف هل البكتيريا
+
+262
+00:20:03,590 --> 00:20:07,290
+عندها هذا الانزيم ولا لأ هو exo enzyme لو
+
+263
+00:20:07,290 --> 00:20:11,290
+البكتيريا عندها انزيم الجيلاتينياز هتكسر الجيلاتين
+
+264
+00:20:11,290 --> 00:20:15,670
+هتحوله ل amino acid زائد small peptide يبقى
+
+265
+00:20:15,670 --> 00:20:19,710
+الجيلاتين كان هو يعتبر solidifying agent هو اللي
+
+266
+00:20:19,710 --> 00:20:23,910
+خلّى ال media صلبة لما اتكسر الجيلاتين صار الوسط
+
+267
+00:20:23,910 --> 00:20:28,730
+سائل liquid يبقى هذا ال principle الفحصإنها الـ
+
+268
+00:20:28,730 --> 00:20:38,430
+media بعد ما كانت صلبة هستير سائلة Determine
+
+269
+00:20:38,430 --> 00:20:42,430
+an organism ability to produce proteolytic-like
+
+270
+00:20:42,430 --> 00:20:46,510
+enzyme and lycophygelatin فقوللي هذا الفحص عشان
+
+271
+00:20:46,510 --> 00:20:51,510
+أعرف هل البكتيريا بتفرز أنزيماتبتعمل البروتيوليتك
+
+272
+00:20:51,510 --> 00:20:56,290
+بتحلل البروتينات وبتخلي الوساط سائل البروتيوليتك
+
+273
+00:20:56,290 --> 00:21:00,430
+يعني license of a protein this test is used to
+
+274
+00:21:00,430 --> 00:21:05,210
+differentiate gram negative species هذه الفقرة هي
+
+275
+00:21:05,210 --> 00:21:08,850
+ال significant احنا قلنا كل فحص فيه له significant
+
+276
+00:21:08,850 --> 00:21:13,050
+مين ال positive و ال negative او بيفرق مين عن مين
+
+277
+00:21:13,050 --> 00:21:16,730
+وليها ده يستخدم to differentiate gram negative
+
+278
+00:21:16,730 --> 00:21:23,770
+speciesCeracea, Sodomonas and Vibrio هدول كلهم
+
+279
+00:21:23,770 --> 00:21:27,590
+positive لفحص الجيلاتين عندهم جيلاتينيز بيكسر
+
+280
+00:21:27,590 --> 00:21:31,150
+الجيلاتين ال test can be used to differentiate
+
+281
+00:21:31,150 --> 00:21:36,390
+genera of gelatinase producing bacteria such as
+
+282
+00:21:36,390 --> 00:21:40,290
+Ceracea and Brutias from other members of the
+
+283
+00:21:40,290 --> 00:21:45,090
+family Enterobacteriaceae بقوللي أن هذا الفحص
+
+284
+00:21:45,090 --> 00:21:49,720
+هيميزالبروتياس والسيراشيا هدول يعتبروا positive
+
+285
+00:21:49,720 --> 00:21:54,180
+للجيلاتيناز عن باقي أعضاء ال انتيرو بكترياس
+
+286
+00:21:54,180 --> 00:21:58,360
+الانتيرو بكترياس عائلة بتضم أكتر من بكتيريا من
+
+287
+00:21:58,360 --> 00:22:03,280
+ضمنها السيراشيا والبروتياس هدول هما فقط ال
+
+288
+00:22:03,280 --> 00:22:06,680
+positive لهذا الفحص من عائلة الانتيرو بكترياس
+
+289
+00:22:06,680 --> 00:22:11,280
+فبالتالي ممكن أن أنا أفرق هدول عن باقي أعضاء
+
+290
+00:22:11,280 --> 00:22:16,890
+الانتيرو بكترياس بفحص الجيلاتينازبقول لي إنه الفحص
+
+291
+00:22:16,890 --> 00:22:20,710
+هذا ال issue العملي لهذا الفحص لحد الآن لم يقدر
+
+292
+00:22:20,710 --> 00:22:26,310
+وفي طرق للكشف عن الانزيم بشكل أسرع هنتعرف عليها
+
+293
+00:22:26,310 --> 00:22:31,370
+خلال المحاضرة Staphylococcus aureus which is
+
+294
+00:22:31,370 --> 00:22:34,750
+gelatinous positive can be differentiated from
+
+295
+00:22:34,750 --> 00:22:38,490
+Staphylococcus epidermidis gelatinous negative
+
+296
+00:22:39,500 --> 00:22:43,460
+الستافيلو كوكاس أورياس جيلتان جيلاتيناز positive
+
+297
+00:22:43,460 --> 00:22:47,240
+لكن الاستافيلو كوكاس ايه بدار مدرس تعتبر جيلاتيناز
+
+298
+00:22:47,240 --> 00:22:52,140
+negative احنا قلنا انه الاستافيلو كوكاي بشكل عام
+
+299
+00:22:52,140 --> 00:22:58,260
+الجنراهاي gram positive كوكاي كتلاز positive تمام
+
+300
+00:22:58,570 --> 00:23:02,350
+الستفيلوكوكاي كتلاز positive عشان افرق ال species
+
+301
+00:23:02,350 --> 00:23:07,190
+عن بعض ذكرنا فحص كيميائي اللي هو ال coagulase
+
+302
+00:23:07,190 --> 00:23:11,110
+قولنا انه الستفيلوكوكاس اورياس coagulase positive
+
+303
+00:23:11,110 --> 00:23:14,490
+لكن الستفيلوكوكاس ابيديرمتاس coagulase negative
+
+304
+00:23:14,490 --> 00:23:18,230
+فحص تاني اللي هو الجيلاتيناز نفس الاشي
+
+305
+00:23:18,230 --> 00:23:22,430
+الستفيلوكوكاس اورياس جيلاتيناز positive لكن
+
+306
+00:23:22,430 --> 00:23:26,610
+الستفيلوكوكاس ابيديرمتاس جيلاتيناز negativeالـ
+
+307
+00:23:26,610 --> 00:23:29,930
+Staphylococcus aureus قلنا هي pathogenic لكن الـ
+
+308
+00:23:29,930 --> 00:23:33,550
+Staphylococcus epidermidis هي abortionistic في
+
+309
+00:23:33,550 --> 00:23:39,670
+كمان فحصين هيمروا معنا خلال الشريح هذا هو المقارنة
+
+310
+00:23:39,670 --> 00:23:42,890
+هما تنتيني ground positive ككاي كتلز positive لـ
+
+311
+00:23:42,890 --> 00:23:45,650
+Staphylococcus aureus لكن في end species لـ
+
+312
+00:23:45,650 --> 00:23:47,850
+Staphylococcus aureus و لـ Staphylococcus
+
+313
+00:23:47,850 --> 00:23:50,590
+epidermidis قلنا الـ Coagulase positive لـ
+
+314
+00:23:50,590 --> 00:23:54,050
+Staphylococcus aureus و الجيلاتيناز positiveفي
+
+315
+00:23:54,050 --> 00:23:57,190
+عندي فحصين هنتعرف عليهم أو هيمروا معانا خلال
+
+316
+00:23:57,190 --> 00:24:01,150
+الشباتر الجاي ال money toll و ال DNS برضه positive
+
+317
+00:24:01,150 --> 00:24:04,370
+في الاستفالة الكوكس او yes لكن negative في
+
+318
+00:24:04,370 --> 00:24:06,190
+الاستفالة الكوكس epidermis
+
+319
+00:24:09,210 --> 00:24:12,990
+Bacillus anthrax and Bacillus cereus and several
+
+320
+00:24:12,990 --> 00:24:17,570
+other members of the genus are gelatinous positive
+
+321
+00:24:17,570 --> 00:24:21,250
+as are Clostridium titani and Clostridium
+
+322
+00:24:21,250 --> 00:24:24,750
+perifringus قلنا إن ال Clostridium و البacillus
+
+323
+00:24:24,750 --> 00:24:29,570
+يعتبروا إجرام positive بسلائي Spore forming بفرق
+
+324
+00:24:29,570 --> 00:24:34,120
+بينهم بفحص الكتلازالبصيلة الـ Sirius والبصيلة
+
+325
+00:24:34,120 --> 00:24:38,740
+الانتراكس بقولهم جيلاتيناز positive وكمان التنتان
+
+326
+00:24:38,740 --> 00:24:43,420
+إذا بدنا نربط المعلومات كانوا يعتبروا central in
+
+327
+00:24:43,420 --> 00:24:47,500
+the endospar الكلستريديوم تيتانيب والكلستريديوم
+
+328
+00:24:47,500 --> 00:24:51,420
+بيرفرنجز برضه يعتبروا جيلاتيناز positive يبقى هذا
+
+329
+00:24:51,420 --> 00:24:54,440
+هو ال significant للفحص
+
+330
+00:24:57,230 --> 00:25:01,310
+Gelatin is a protein بقولّي هو عبارة عن بروتين
+
+331
+00:25:01,310 --> 00:25:06,270
+derived from collagen هو عبارة عن بروتين مشتاق من
+
+332
+00:25:06,270 --> 00:25:09,610
+الـ collagen الموجود في الـ connective tissue
+
+333
+00:25:09,610 --> 00:25:14,090
+Gelatinase comprise a family of extracellular
+
+334
+00:25:14,090 --> 00:25:18,070
+enzyme قلنا جيلاتيناز يعتبر من الـ exoenzyme
+
+335
+00:25:18,070 --> 00:25:22,610
+produced and secreted by some microorganism قلنا
+
+336
+00:25:22,610 --> 00:25:26,760
+بيتم إنتاجهبواسطة بعض أنواع البكتيريا مش كل
+
+337
+00:25:26,760 --> 00:25:30,540
+البكتيريا عندها أنزيم الجيلاتيناز to hydrolyze
+
+338
+00:25:30,540 --> 00:25:34,800
+الجيلاتين عشان يتكسر الجيلاتين ويتحول ل amino acid
+
+339
+00:25:34,800 --> 00:25:39,340
+هذا ال amino acid يعتبر nitrogen source يستخدم في
+
+340
+00:25:39,340 --> 00:25:44,340
+إنتاج البروتين جيلاتين is a simple protein اتفقنا
+
+341
+00:25:44,340 --> 00:25:48,680
+هو عبارة عن simple protein هذه المعلومة مهمة جدا
+
+342
+00:25:48,680 --> 00:25:52,460
+بقول لي أن الجيلاتين بكون solid لما تكون درجة
+
+343
+00:25:52,460 --> 00:25:58,220
+الحرارةخمسة و عشرين درجة سليزيوسية او اقل بيكون
+
+344
+00:25:58,220 --> 00:26:01,700
+liquid لما تكون درجة الحرارة ستة و عشرين درجة
+
+345
+00:26:01,700 --> 00:26:07,020
+سليزيوس او اعلى يبقى الجيلاتين بتتغير حالته حسب
+
+346
+00:26:07,020 --> 00:26:11,380
+درجة الحرارة وهي معلومة مهمة جدا و ال bacteria
+
+347
+00:26:11,380 --> 00:26:15,060
+that produce the enzyme gelatinase are grown in a
+
+348
+00:26:15,060 --> 00:26:19,570
+gelatin media the enzyme break upThe gelatin
+
+349
+00:26:19,570 --> 00:26:23,390
+molecule and the medium cannot solidify even at
+
+350
+00:26:23,390 --> 00:26:27,590
+cold temperature هذه الجملة أو الفقرة هي ال
+
+351
+00:26:27,590 --> 00:26:31,050
+principle للفحص بولي أنا لو نميت البكتيريا اللي
+
+352
+00:26:31,050 --> 00:26:35,770
+عندها ال exoenzyme الجيلاتينايز في وسط فيه جيلاتين
+
+353
+00:26:35,770 --> 00:26:39,800
+اللي هو ال substrateأكيد الانزيم الجيلاتيناز
+
+354
+00:26:39,800 --> 00:26:43,780
+الموجود في البكتيريا هيشتغل على ال substrate ال
+
+355
+00:26:43,780 --> 00:26:46,960
+substrate اللي هي الجيلاتين الموجود في ال media
+
+356
+00:26:46,960 --> 00:26:51,400
+هيكسر الجيلاتين اللي هو solid هيحوله ل amino acid
+
+357
+00:26:51,400 --> 00:26:56,440
+زائد small peptide هيبطل الوسط صلب هيصير الوسط
+
+358
+00:26:56,440 --> 00:27:00,320
+سائل يبقى ال media مش هتقدر تتصلب حتى في ال cold
+
+359
+00:27:00,320 --> 00:27:04,360
+temperature طب ليش قاللي حتى في ال cold
+
+360
+00:27:04,360 --> 00:27:04,980
+temperature
+
+361
+00:27:07,390 --> 00:27:11,090
+لأنه بالجملة اللى قبلها قالنا ان الجيلاتين بكون
+
+362
+00:27:11,090 --> 00:27:16,630
+صلب في درجات الحرارة المنخفضة عند 25 درجة سيليزيا
+
+363
+00:27:16,630 --> 00:27:21,650
+او اقل يعني بكون صلب في الكوال تمريتشار طيب بما
+
+364
+00:27:21,650 --> 00:27:25,330
+انه اتكسر الجيلاتين بطل عندى جيلاتين صار amino
+
+365
+00:27:25,330 --> 00:27:29,730
+acid يبقى مش هتتصلى بال media حتى لو كانت درجة
+
+366
+00:27:29,730 --> 00:27:33,770
+الحرارة منخفضة two methods used to determine
+
+367
+00:27:33,770 --> 00:27:37,940
+gelatinase production بقولى فيه عندىطريقتين لكشف
+
+368
+00:27:37,940 --> 00:27:41,640
+على ال gelatinous production طريقة الأولى gelatin
+
+369
+00:27:41,640 --> 00:27:45,540
+stab method الطريقة التانية gelatin strip method
+
+370
+00:27:45,540 --> 00:27:53,860
+من نضيف طريقة تالتة وهي le charcoal طريقة
+
+371
+00:27:53,860 --> 00:27:58,060
+تالتة وهي اسمها le charcoal method
+
+372
+00:28:10,660 --> 00:28:19,700
+التشاركول method يبقى
+
+373
+00:28:19,700 --> 00:28:22,440
+الطريقة الأولى الجيلاتين step method الجيلاتين
+
+374
+00:28:22,440 --> 00:28:26,220
+strip method التشاركول method هنتعرف على كل طريقة
+
+375
+00:28:26,220 --> 00:28:30,040
+شو ال principle اللي لها شو طريقة الشغل وكيف بتكون
+
+376
+00:28:30,040 --> 00:28:30,580
+ال result
+
+377
+00:28:35,040 --> 00:28:38,640
+في هاي ال slide ذاكر Sirachia Sodomonas Vibrio
+
+378
+00:28:38,640 --> 00:28:45,120
+كلهم يعتبروا جيلاتيناز positive أول طريقة عشان
+
+379
+00:28:45,120 --> 00:28:48,960
+أكشف على الجيلاتيناز هي ال gelatin stab method
+
+380
+00:28:48,960 --> 00:28:54,740
+الطريقة هي أني أنا بجيب التيوب طبعا مكتوب عندي أنا
+
+381
+00:28:54,740 --> 00:28:59,540
+أستابي بدي أعمل stabbing طاعن هتكون في التيوب
+
+382
+00:28:59,710 --> 00:29:04,870
+Hyalite-U بتحتوي على nutrient بروث البروث وليس
+
+383
+00:29:04,870 --> 00:29:09,990
+أجار ضروري جدا أن تكون البروث مش أجار لأن الأجار
+
+384
+00:29:09,990 --> 00:29:14,250
+يعتبر solidifying agent هتظلها صليت سواء كانت
+
+385
+00:29:14,250 --> 00:29:18,670
+البكتيريا positive أو negative للجيلاتيناز يبقى
+
+386
+00:29:18,670 --> 00:29:24,030
+ممنوع أحط أجار لأن لو حطت أجار ال media هتظل صليت
+
+387
+00:29:24,030 --> 00:29:26,230
+سواء كانت البكتيريا positive أو negative
+
+388
+00:29:26,230 --> 00:29:33,710
+للجيلاتينازهضيف 12% جيلاتين يبقى الـ 12% الجيلاتين
+
+389
+00:29:33,710 --> 00:29:38,910
+هيكون كsubstrate كيف بدى انا احضر هذه ال media
+
+390
+00:29:38,910 --> 00:29:44,250
+بقوللي بتجيبي ال nutrient broth تمام تقرا ايه شو
+
+391
+00:29:44,250 --> 00:29:48,270
+التعليمات الموجودة على البطولة كنت كتبلي انه كل
+
+392
+00:29:48,270 --> 00:29:52,190
+1000 ملي ضيف عليها 13 جرام من ال nutrient broth طب
+
+393
+00:29:52,190 --> 00:29:56,860
+انا بدى احضر 500 مل بعمل ضرب تبادلىبيطلع ان انا
+
+394
+00:29:56,860 --> 00:30:00,860
+بدي 6.5 جرام من الـ nutrient broth طيب نسبة
+
+395
+00:30:00,860 --> 00:30:06,400
+الجيلاتين بقوللي المستخدمة في هذا الفحص 12% 12%
+
+396
+00:30:06,400 --> 00:30:11,900
+يعني 12 لكل 100 مل دستيل ووتر طب انا اضيفت 500 مل
+
+397
+00:30:11,900 --> 00:30:16,220
+بعمل درد تبادل يبقى بدي 60 جرام of جيلاتين يبقى
+
+398
+00:30:16,220 --> 00:30:20,540
+بضيف ال 60 جرام من الجيلاتين على 6.5 جرام من ال
+
+399
+00:30:20,540 --> 00:30:25,720
+nutrient brothبعملهم mix فيه 500 مل دستل واتر على
+
+400
+00:30:25,720 --> 00:30:30,380
+الـ hot plate بعملهم mix بحطهم على ال hot plate
+
+401
+00:30:30,380 --> 00:30:35,480
+بعد ما يدوبوا منيح بحطهم فيه تيوب بحط سواب بدخلهم
+
+402
+00:30:35,480 --> 00:30:40,040
+في ال auto clef ال auto clef هيعقم للميديا بستنها
+
+403
+00:30:40,040 --> 00:30:44,540
+تبرد وبعدين بشتغل الفحص لتيوب بعد ما تطلع بسميها
+
+404
+00:30:44,540 --> 00:30:51,200
+nutrient gelatin deep tubeتمام هذه بتحتوي على ال
+
+405
+00:30:51,200 --> 00:30:55,000
+substrate اللي هي الجيلاتين باجي باخد البكتيريا
+
+406
+00:30:55,000 --> 00:30:58,640
+اللي انا بدي اكشف عندها انزيم الجيلاتيناز او لأ
+
+407
+00:30:58,640 --> 00:31:02,020
+يبقى touch of بكتيريا من الطبق اللي انا بدي افحص
+
+408
+00:31:02,020 --> 00:31:04,320
+او البكتيريا اللي بدي افحصها لها انت الانزيم ولا
+
+409
+00:31:04,320 --> 00:31:07,280
+لأ يبقى عند ال substrate اللي موجود في ال media
+
+410
+00:31:07,280 --> 00:31:10,720
+اللي هو الجيلاتين بدي اكشف عن الانزيم اللي موجود
+
+411
+00:31:10,720 --> 00:31:14,760
+في البكتيريا او مش موجود لسه مش عارفة هعمل
+
+412
+00:31:14,760 --> 00:31:20,320
+stabbing هي هتكون deep زي فحص ال motilityهعقم
+
+413
+00:31:20,320 --> 00:31:24,540
+الفوقة و هدخل بشكل عمودي و هعمل stabbing هعمل طعنة
+
+414
+00:31:24,540 --> 00:31:29,240
+في النص تقريبا 3 تولتين و هطلع هعقم الفوقة هعمل
+
+415
+00:31:29,240 --> 00:31:37,080
+incubation لمدة أسبوع على 37 درجة سليسيا بعد ما
+
+416
+00:31:37,080 --> 00:31:42,600
+أعمل incubation بقوللي بحطيها في التلاجة لمدة 30
+
+417
+00:31:42,600 --> 00:31:47,660
+دقيقة هنا سؤال مهمليش انا بدي احطها في التلاجة
+
+418
+00:31:47,660 --> 00:31:53,520
+لمدة 30 دقيقة بقوللي لما طليها من ال incubation
+
+419
+00:31:53,520 --> 00:31:57,860
+سواء كان البكتيريا عندها أنزيم الجيلاتيناز او ما
+
+420
+00:31:57,860 --> 00:32:02,460
+عندها هتكون liquid ليش؟ لو كانت البكتيريا عندها
+
+421
+00:32:02,460 --> 00:32:06,700
+أنزيم الجيلاتيناز هتكون liquid لأنه الجيلاتيناز
+
+422
+00:32:06,700 --> 00:32:10,440
+كسر الجيلاتين اتحول الوسط من solid ل liquid حسب
+
+423
+00:32:10,440 --> 00:32:15,060
+المعادلة اللي ذكرناها في أول slide طيب
+
+424
+00:32:16,490 --> 00:32:22,630
+لو ما عندها انزيم الجيلاتيناز ليش ضلت او ليش كانت
+
+425
+00:32:22,630 --> 00:32:27,130
+liquid بقولي لو كانت ما عندها انزيم الجيلاتيناز
+
+426
+00:32:27,130 --> 00:32:32,930
+تمام الجيلاتين مش هيتكسر تمام هيضل الجيلاتين في ال
+
+427
+00:32:32,930 --> 00:32:37,550
+media لكن احنا قولنا في ال slide ان الجيلاتين في
+
+428
+00:32:37,550 --> 00:32:45,200
+درجات الحرارة لما يكون من 25 او اعلى بتكوناللي
+
+429
+00:32:45,200 --> 00:32:49,560
+بتكون حالته liquid طب درجة ال incubator حرارة ال
+
+430
+00:32:49,560 --> 00:32:54,360
+incubator 37 يبقى أعلى من 26 يبقى هيكون liquid
+
+431
+00:32:54,360 --> 00:32:59,880
+فبالتالي بقولنا بنحطها في التلاجة لمدة 30 دقيقة
+
+432
+00:32:59,880 --> 00:33:07,190
+عشان أعرف و أحدد هل ال liquid نتيجة تكسيرالانزيم
+
+433
+00:33:07,190 --> 00:33:11,110
+الجيلاتيناز للجيلاتين واتحول لـ liquid ولا لأنه
+
+434
+00:33:11,110 --> 00:33:16,070
+كان ال incubator درجة حرارته أعلى من 26 فالجيلاتين
+
+435
+00:33:16,070 --> 00:33:21,530
+صار في الحالة السائلة فاحطه في التلاجة لمدة 30
+
+436
+00:33:21,530 --> 00:33:26,390
+minute هشوف إذا ضلوا liquid يبقى النتيجة بتكون
+
+437
+00:33:26,390 --> 00:33:30,030
+positive فعندها انزيم الجيلاتيناز كسر الجيلاتين
+
+438
+00:33:30,030 --> 00:33:37,450
+أعطاني أمينو أسد اتحول الوسط ل liquid لكنلو اتحول
+
+439
+00:33:37,450 --> 00:33:42,890
+ل solid بقول لي ما بكتب negative اذا متذكرين فحص
+
+440
+00:33:42,890 --> 00:33:45,790
+الـ coagulase في ال tube method كنا نقول نفس
+
+441
+00:33:45,790 --> 00:33:50,870
+الفكرة بدي اعمل re incubation for total 30 days
+
+442
+00:33:50,870 --> 00:33:56,430
+بدي اعمل اكمل incubation اكمل ل 30 يوم احنا عملنا
+
+443
+00:33:56,430 --> 00:34:04,330
+أسبوع سبع أيام فانا بكمل 23 يوم incubation عشانفي
+
+444
+00:34:04,330 --> 00:34:08,910
+بعض أنواع البكتيريا بيكون عندها الانزيم بكميات
+
+445
+00:34:08,910 --> 00:34:14,050
+قليلة فبالتالي عشان تقدر تكسر الجيلاتين بياخد وقت
+
+446
+00:34:14,050 --> 00:34:18,610
+فأنا عشان أضمن أنه هذه البكتيريا لو كان في عندي
+
+447
+00:34:18,610 --> 00:34:21,750
+هذه البكتيريا بتفرز الانزيم بكميات قليلة عشان أضمن
+
+448
+00:34:21,750 --> 00:34:27,510
+هذا الاشي بخليها incubation لمدة 30 يومبعد ما يخلص
+
+449
+00:34:27,510 --> 00:34:32,570
+الـ 30 يوم بحطها في التلاجة لمدة 30 minute لنفس
+
+450
+00:34:32,570 --> 00:34:36,490
+السبب اللى ذكرناه قبل شوية لأنه سواء كانت positive
+
+451
+00:34:36,490 --> 00:34:39,870
+أو negative هتكون liquid لأنه درجة حرارة ال
+
+452
+00:34:39,870 --> 00:34:45,310
+incubator 37 لو كانت negative فبالتالي الجيلاتين
+
+453
+00:34:45,310 --> 00:34:48,550
+فيه لما تكون درجة الحرارة أعلى من 26 هيكون في
+
+454
+00:34:48,550 --> 00:34:52,610
+الحالة في ال liquid في الحالة السائلة لو كان عندها
+
+455
+00:34:52,610 --> 00:34:57,850
+ال enzyme أكيد هيتكسر فانا مش عارفةهل عند الانزيم
+
+456
+00:34:57,850 --> 00:35:04,810
+ولا لأ فأحطها في التلاجة لمدة 30 دقيقة إذا دلت
+
+457
+00:35:04,810 --> 00:35:08,930
+liquid يبقى هي positive لكن لو اتصلبت يبقى هي
+
+458
+00:35:08,930 --> 00:35:13,010
+negative نسجل النتيجة negative هذا بالنسبة
+
+459
+00:35:13,010 --> 00:35:17,110
+للجليلاتين step method الفكرة ال principle زي ما
+
+460
+00:35:17,110 --> 00:35:22,950
+قلنا انه الوسط بتتحول ل liquid هذا ال principle
+
+461
+00:35:23,500 --> 00:35:28,860
+وبالنسبة لـ Result إما Liquid أو Solid الـ Result
+
+462
+00:35:28,860 --> 00:35:33,720
+في الجيلاتين استعب ميثود لو كانت نتيجة Positive
+
+463
+00:35:33,720 --> 00:35:38,020
+بيكون Liquid بتنتج وينتهي عن أنظم الجيلاتيناز ممكن
+
+464
+00:35:38,020 --> 00:35:41,480
+يكون Strong Positive وممكن تكون Weak Positive
+
+465
+00:35:41,480 --> 00:35:46,040
+Strong Positive إنها بتتحول لـ Liquid خلال تلت
+
+466
+00:35:46,040 --> 00:35:50,840
+أياملكن لو كانت week positive خلال من تلت لتلتين
+
+467
+00:35:50,840 --> 00:35:54,420
+يوم بيصير عندى اللى هو بتحول الوسط ل liquid زى
+
+468
+00:35:54,420 --> 00:35:59,380
+البروتياز قولنا انه بيكون عندها الانزين الانزين
+
+469
+00:35:59,380 --> 00:36:03,220
+بكميات قليلة ال negative ان الوسط بيصير له
+
+470
+00:36:03,220 --> 00:36:07,460
+solidify بتصلب نتيجة ان الجيلاتين ما تكسر دلو
+
+471
+00:36:07,460 --> 00:36:12,640
+الجيلاتينمع تأنزيب الـ Gelatinase حتى بعد 30 يوم
+
+472
+00:36:12,640 --> 00:36:17,600
+بتظلها الـ media still solid هذا بالنسبة لنتائج
+
+473
+00:36:17,600 --> 00:36:24,080
+طريقة الـ Gelatin Stab Methodنمر على الـ slides في
+
+474
+00:36:24,080 --> 00:36:27,660
+الجيلاتين step method بقوللي انكيولات نيوتريان
+
+475
+00:36:27,660 --> 00:36:31,900
+جيلاتين ييب اتيوب بتحتوي على 12% جيلاتين بعملها
+
+476
+00:36:31,900 --> 00:36:36,740
+incubation لمدة أسبوع بحدد إذا صار في عندي
+
+477
+00:36:36,740 --> 00:36:40,880
+liquification أنه اتحول ال media ل liquid ولا لأ
+
+478
+00:36:40,880 --> 00:36:47,700
+أو بحطها في التلاجة لمدة 30 دقيقةبعدها بشوف بعد ما
+
+479
+00:36:47,700 --> 00:36:51,020
+اتخلص التلاتين دقيقة بتأكد انه صار في عندي
+
+480
+00:36:51,020 --> 00:36:54,600
+leakification ولا لأ بقول لو negative بتكملي
+
+481
+00:36:54,600 --> 00:36:59,140
+incubation لتلاتين يوم if the gelatin is still
+
+482
+00:36:59,140 --> 00:37:02,640
+intact لو الجيلاتين ضاله صلب يبقى ال bacteria
+
+483
+00:37:02,640 --> 00:37:06,540
+didn't produce gelatinase البكتيريا ما عندها أنزيم
+
+484
+00:37:06,540 --> 00:37:11,030
+الجيلاتيناز ال media will solidifyالـ media بتتصلب
+
+485
+00:37:11,030 --> 00:37:14,250
+في التلاجة و الـ negative result أنا بسجلها
+
+486
+00:37:14,250 --> 00:37:18,330
+negative result إنه لو أنا حاطيتها في التلاجة تمام
+
+487
+00:37:18,330 --> 00:37:24,350
+ال media هتتصلب لأنه درجة الحرارة أقل من 25 قولنا
+
+488
+00:37:24,350 --> 00:37:28,310
+إنه الجيلاتين بتغير حسب درجة الحرارة if the
+
+489
+00:37:28,310 --> 00:37:32,410
+organism has produced sufficient gelatinase لو كان
+
+490
+00:37:32,410 --> 00:37:36,970
+عند الorganism انزيم الجيلاتينازالتيوب سيبقى
+
+491
+00:37:36,970 --> 00:37:40,790
+مليكود حتى
+
+492
+00:37:40,790 --> 00:37:42,630
+لو انا حطيته في التلاجة
+
+493
+00:37:49,500 --> 00:37:53,920
+إذا بنلاحظ أنه أنا ميّلت فبت .. ميّلت ال .. التيوب
+
+494
+00:37:53,920 --> 00:37:58,960
+فالتيوب ما اتحرك يبقى ضاله الوسط solid يبقى هي
+
+495
+00:37:58,960 --> 00:38:03,260
+negative gelatinase لكنها ميّلت التيوب يبقى اتحرك
+
+496
+00:38:03,260 --> 00:38:08,920
+يبقى الوسط سائل يبقى هي positive gelatinase some
+
+497
+00:38:08,920 --> 00:38:13,150
+organism may produce gelatinasein rather small
+
+498
+00:38:13,150 --> 00:38:18,030
+quantities قلنا في بعض أنواع البكتيريا تفرز انزيم
+
+499
+00:38:18,030 --> 00:38:22,210
+الجيلاتيناز بكميات قليلة عشان هيك ال negative
+
+500
+00:38:22,210 --> 00:38:26,150
+gelatinase لازم اني انا عاملها re incubated for 30
+
+501
+00:38:26,150 --> 00:38:30,850
+days لمدة 30 يوم لازم اعمل incubation عشان اتأكد
+
+502
+00:38:30,850 --> 00:38:38,310
+انه هي negative result بالنسبة
+
+503
+00:38:38,310 --> 00:38:42,640
+لهذا ال slide قلنا السيراشياعبارة عن positive
+
+504
+00:38:42,640 --> 00:38:46,680
+للجيلاتين بقدر افرق بينها وبين باقي الـ
+
+505
+00:38:46,680 --> 00:38:50,400
+Enterobacteriasis سلمونيلا هي عبارة عن من عالة الـ
+
+506
+00:38:50,400 --> 00:38:52,720
+Enterobacteriasis قولنا احنا فقط من عالة الـ
+
+507
+00:38:52,720 --> 00:38:56,100
+Enterobacteriasis الجيلاتين positive السيراشيا و
+
+508
+00:38:56,100 --> 00:38:59,320
+البروتياز يبقى السلمونيلا هي بتكون negative
+
+509
+00:38:59,320 --> 00:39:04,040
+الميديا اتصلبت لكن هنا في السيراشيا هي positive
+
+510
+00:39:04,040 --> 00:39:08,520
+للجيلاتين بقولنا ان الميديا صارت liquid سائلة اذا
+
+511
+00:39:08,520 --> 00:39:13,600
+ملاحظها اليومفي عندي سائل اتحرك لطيوب لكنها صلبة
+
+512
+00:39:13,600 --> 00:39:20,480
+الميديا يبقى هي بتكون negative للجيلاتينيز النتائج
+
+513
+00:39:20,480 --> 00:39:23,660
+قلنا ممكن انها تكون positive strong او weak ال
+
+514
+00:39:23,660 --> 00:39:27,120
+strong خلال تلات أيام ال week من تلاتة لتلاتين يوم
+
+515
+00:39:27,120 --> 00:39:31,320
+ال negative حتى بعد تلاتين يوم ما بيصير في عندي
+
+516
+00:39:31,320 --> 00:39:34,360
+liquification بتظلها ال media solid
+
+517
+00:39:38,650 --> 00:39:42,750
+هذه الطريقة الأولى شرحناها نجلى الطريقة التانية
+
+518
+00:39:42,750 --> 00:39:45,910
+اللى هى ال gelatin strip method ال gelatin strip
+
+519
+00:39:45,910 --> 00:39:49,190
+method بقولنا بنجيب تيوب بنحط فيها bacterial
+
+520
+00:39:49,190 --> 00:39:51,670
+suspension اش يعنى bacterial suspension يعني
+
+521
+00:39:51,670 --> 00:39:54,690
+normal saline مع بكتيريا أو nutrient broth مع
+
+522
+00:39:54,690 --> 00:39:57,790
+بكتيريا طبعا البكتيريا اللى بدى اكشف عندها الانزيم
+
+523
+00:39:57,790 --> 00:40:02,450
+ولا لأ بقولنا بنضيف X-ray film ال X-ray film
+
+524
+00:40:02,450 --> 00:40:06,210
+بقولنا هى coated with green gelatin emulsion يبقى
+
+525
+00:40:06,210 --> 00:40:10,490
+هتكون هى ال substrateالـ X-ray film بتحتوي على
+
+526
+00:40:10,490 --> 00:40:13,370
+الجيلاتين يبقى هي السبسترات اللي هيشتغل عليها
+
+527
+00:40:13,370 --> 00:40:16,210
+الانزيم الموجود في البكتيريا او مش موجود انا بدي
+
+528
+00:40:16,210 --> 00:40:22,870
+اكشف عنه بعدها بعمل incubation على درجة 37 لمدة 4
+
+529
+00:40:22,870 --> 00:40:27,590
+ساعات لو البكتيريا عندها انزيم الجيلاتينازهتشتغل
+
+530
+00:40:27,590 --> 00:40:32,370
+على الجيلاتين الموجود بالـ X-ray و هتحاول انها ايش
+
+531
+00:40:32,370 --> 00:40:36,470
+تكسر هذا الجيلاتين فبالتالي اللون الأسود اللي
+
+532
+00:40:36,470 --> 00:40:42,250
+موجود على الفيلم لـ X-ray هيختفي و هيصير colorless
+
+533
+00:40:42,250 --> 00:40:46,010
+يبقى النتيجة ال positive انه الـ X-ray فيلم هيسله
+
+534
+00:40:46,010 --> 00:40:49,490
+دي سبير هيصير colorless لكن لو ضاله لونه أسود
+
+535
+00:40:49,490 --> 00:40:53,740
+بقوليماتسجل النتيجة negative بتعمل Re-incubation
+
+536
+00:40:53,740 --> 00:40:58,660
+for a total of 24 hours بتكمل الـ Incubation لـ 24
+
+537
+00:40:58,660 --> 00:41:02,420
+ساعة يبقى لما أنك احنا حطينا بالأول 4 ساعات بنعمل
+
+538
+00:41:02,420 --> 00:41:06,300
+الـ Incubation لمدة 20 ساعة لو دلوا لونه أسود يبقى
+
+539
+00:41:06,300 --> 00:41:12,020
+خلاص بسجل النتيجة negative لكن لو اتغير لـ الـ
+
+540
+00:41:12,020 --> 00:41:15,540
+Nesapir اختفى اللون الأسود وصار colorless يبقى هي
+
+541
+00:41:15,540 --> 00:41:20,070
+positive للجيلاتيناز لكن أخدت وقتلأنه اللي قلنا في
+
+542
+00:41:20,070 --> 00:41:24,850
+بعض أنواع البكتيريا بتفرز الانزيم بكميات قليلة هذا
+
+543
+00:41:24,850 --> 00:41:28,630
+بالنسبة لطريقة ال gelatin strip method هي
+
+544
+00:41:28,630 --> 00:41:31,710
+alternative method for detecting gelatinase
+
+545
+00:41:31,710 --> 00:41:35,190
+production طريقة تانية عشان أفحص عن الجيلاتيناز
+
+546
+00:41:35,190 --> 00:41:40,080
+باستخدم ال X-ray film ال gelatin stripبقولنا
+
+547
+00:41:40,080 --> 00:41:44,600
+الجيلاتين الـ X-ray film that is coated with a
+
+548
+00:41:44,600 --> 00:41:48,140
+green gelatin emulsion هي بتكون فيها اللي هو
+
+549
+00:41:48,140 --> 00:41:52,480
+الجيلاتين organism that produce gelatinase remove
+
+550
+00:41:52,480 --> 00:41:56,080
+بقولّي لو البكتيريا عندها أنزيم الجيلاتيناز هتزيل
+
+551
+00:41:56,080 --> 00:41:59,780
+ال emulsion from the strip هتزيل اللون الأسود من
+
+552
+00:41:59,780 --> 00:42:03,000
+ال strip كف الطريقة زي ما قلنا بعمل انكولات
+
+553
+00:42:03,000 --> 00:42:08,790
+للبكتيريا في نص مل من ال normal salineلازم يكون ال
+
+554
+00:42:08,790 --> 00:42:11,770
+suspension تربط بعدين بحط ال X-ray film أو
+
+555
+00:42:11,770 --> 00:42:16,330
+الجلاتين في ال bacterial suspension أو ال saline
+
+556
+00:42:16,330 --> 00:42:20,610
+suspension بعمل incubation لمدة أربع ساعات لأربع
+
+557
+00:42:20,610 --> 00:42:25,690
+عشرين ساعة عشان ألاحظ إزالة اللي هو الجلاتين
+
+558
+00:42:25,690 --> 00:42:34,070
+emulsion من ال strip من اللاحظ في هذه الصورةما
+
+559
+00:42:34,070 --> 00:42:38,650
+اختفى اللون الأسود من الـ Strip يبقى هذه النتيجة
+
+560
+00:42:38,650 --> 00:42:43,370
+Negative للجيلاتيناز لكن هنا نلاحظ أن الـ Strip
+
+561
+00:42:43,370 --> 00:42:47,370
+راح اللون الأسود منه يبقى هذه جيلاتيناز Positive
+
+562
+00:42:48,140 --> 00:42:52,180
+الجزء اللى فوق طبعا أكيد ما راح اللون لأنه ما صار
+
+563
+00:42:52,180 --> 00:42:55,980
+فيه touch بينه وبين البكتيريا البكتيريا موجودة في
+
+564
+00:42:55,980 --> 00:42:59,960
+ال suspension في السقل اللى تحت فأكيد اللى صار فيه
+
+565
+00:42:59,960 --> 00:43:03,400
+touch هو اللى هيروح هيصير التفاعل مع ال substrate
+
+566
+00:43:03,400 --> 00:43:06,540
+وهيروح اللون من ال strip يبقى high gelatinase
+
+567
+00:43:06,540 --> 00:43:11,710
+positive وhigh gelatinase negativeخلصنا طريقة اللى
+
+568
+00:43:11,710 --> 00:43:14,990
+هى الـ Gelatin Strip نجى لآخر طريقة اللى هى ال
+
+569
+00:43:14,990 --> 00:43:19,470
+charcoal method ال charcoal هو الفحم الفكرة ان
+
+570
+00:43:19,470 --> 00:43:25,070
+الفحم جزيئات ومتماسكة وسبب وجود الجيلاتين فيه يبقى
+
+571
+00:43:25,070 --> 00:43:27,890
+بجيب انا bacterial suspension normal saline مع
+
+572
+00:43:27,890 --> 00:43:31,270
+بكتيريا او nutrient broth مع بكتيريا البكتيريا بدي
+
+573
+00:43:31,270 --> 00:43:35,330
+اكشف موجود فيها الانزيم ولا لأ فجيب charcoal فحم و
+
+574
+00:43:35,330 --> 00:43:39,480
+احطه فيه high lithium بدل ال X-ray filmالقطش
+
+575
+00:43:39,480 --> 00:43:43,900
+charcoal هيكون في السبستريات تمام هعمل incubation
+
+576
+00:43:43,900 --> 00:43:49,100
+لمدة 24 ساعة على 37 درجة سيليزيا اذا لاحظت انه
+
+577
+00:43:49,100 --> 00:43:52,480
+الفحم اترسب في قاع التيوب يبقى النتيجة negative
+
+578
+00:43:52,480 --> 00:43:57,020
+لكن اذا لاحظت انه الفحم اتوزع في كل التيوب اتفكك
+
+579
+00:43:57,020 --> 00:44:01,730
+الفحم هيصير انتشار في كل تيوبهيتغير لون التيوب لأ
+
+580
+00:44:01,730 --> 00:44:06,350
+اللون الأسود يبقى نتيجة positive لأن البكتيريا
+
+581
+00:44:06,350 --> 00:44:10,370
+عندها انزيم الجيلاتيناز كسر الجيلاتين الجيلاتين هو
+
+582
+00:44:10,370 --> 00:44:14,130
+اللي بيخلّي الفحم متماسك طب الجيلاتين اتكسر راح
+
+583
+00:44:14,130 --> 00:44:17,910
+يبقى الفحم هينتشر في كل تيوب وهيصير لونه أسود يبقى
+
+584
+00:44:17,910 --> 00:44:21,710
+النتيجة ال positive distribution هيصير في يدي لون
+
+585
+00:44:21,710 --> 00:44:25,810
+أسود في كل تيوب لكن نتيجة ال negative مش هتفرز
+
+586
+00:44:25,810 --> 00:44:30,250
+انزيم الجيلاتيناز يبقى الجيلاتين هيضلهبين جزيئات
+
+587
+00:44:30,250 --> 00:44:34,790
+الفحم فبالتالي هيترسب في القاهة هذا فكرة let's
+
+588
+00:44:34,790 --> 00:44:41,410
+share cool هكذا خلصنا طحس الجيلاتيناز وخلصنا ال
+
+589
+00:44:41,410 --> 00:44:44,770
+chapter اللي كان بيتكلم عن الاميلاز and جيلاتيناز
+
+590
+00:44:44,770 --> 00:44:50,270
+وقلنا اتنين exoenzyme بتمنى تكون الأمور واضحة
+
+591
+00:44:50,270 --> 00:44:53,070
+والسلام عليكم ورحمة الله وبركاته
+
diff --git a/PL9fwy3NUQKwZWgAb_V1ATlRRsH-6i3cPC/YZtUaTNI_xw.srt b/PL9fwy3NUQKwZWgAb_V1ATlRRsH-6i3cPC/YZtUaTNI_xw.srt
new file mode 100644
index 0000000000000000000000000000000000000000..0e04cc58692254dfafe666b4308270a1fd4c8f31
--- /dev/null
+++ b/PL9fwy3NUQKwZWgAb_V1ATlRRsH-6i3cPC/YZtUaTNI_xw.srt
@@ -0,0 +1,1679 @@
+1
+00:00:01,960 --> 00:00:05,640
+السلام عليكم ورحمة الله وبركاته يعطيكم العافية
+
+2
+00:00:05,640 --> 00:00:09,080
+جميعًا في هذا اللقاء هنتكلم عن the Catalase and
+
+3
+00:00:09,080 --> 00:00:13,620
+Coagulase Test قلنا إن البكتيريا Small and
+
+4
+00:00:13,620 --> 00:00:17,300
+Colorless  تغلبنا على إنها Small باستخدام
+
+5
+00:00:17,300 --> 00:00:20,520
+الميكروسكوب، الميكروسكوب عمل magnification
+
+6
+00:00:20,520 --> 00:00:25,320
+للبكتيريا، وإقدرت إني أنا أشوفها بالنسبة لإنها
+
+7
+00:00:25,320 --> 00:00:30,080
+Colorless شفافة، حَلّت هذه المشكلة باستخدام الstain
+
+8
+00:00:30,080 --> 00:00:33,920
+باستخدام الصبغة، بتعرفنا على أنواع الصبغات اللي من
+
+9
+00:00:33,920 --> 00:00:38,360
+ضمنها الـ Gram Stain، الـ Gram Stain كانت أول خطوة من
+
+10
+00:00:38,360 --> 00:00:42,180
+خطوات الـ identification، أول خطوة من خطوات تعريف
+
+11
+00:00:42,180 --> 00:00:47,320
+البكتيريا، الـ Gram Stain قسمت البكتيريا لمجموعات،  لـ
+
+12
+00:00:47,320 --> 00:00:51,020
+Gram Positive Bacteria و Gram Negative Bacteria، لـ
+
+13
+00:00:51,020 --> 00:00:55,100
+Gram Positive Bacteria بتضم مجموعة من البكتيريا، و
+
+14
+00:00:55,100 --> 00:00:58,980
+كذلك لـ Gram Negative، عشان أوصل للتعريف النهائي
+
+15
+00:00:59,210 --> 00:01:04,030
+لازم أعمل فحوصات كيميائية Biochemical Test، واليوم
+
+16
+00:01:04,030 --> 00:01:09,130
+هنتكلم عن أول فحصين كيميائيين، أهمهم الـ Catalase and
+
+17
+00:01:09,130 --> 00:01:14,670
+Coagulase Test، عشان نوضح فكرة الفحوصات الكيميائية
+
+18
+00:01:14,670 --> 00:01:20,030
+لو أنتَ بدك تقابل بنت لأول مرة، وعرفتي مواصفات هذه
+
+19
+00:01:20,030 --> 00:01:24,130
+البنت، أكيد لما توصلي للمكان اللي هتتقابليه فيه
+
+20
+00:01:24,130 --> 00:01:27,350
+وقتها تشوفي مين في المكان ليه نفس هذه المواصفات
+
+21
+00:01:27,350 --> 00:01:33,670
+وتستفيد، لما توصلي للبنت اللي أنت بدك إياها، يبقى
+
+22
+00:01:33,670 --> 00:01:37,990
+جمعتي أكثر من صفة لهذه البنت عشان تعرفيها، نفس
+
+23
+00:01:37,990 --> 00:01:41,950
+الشغل مع البكتيريا، عشان أوصل للتعريف النهائي بدي
+
+24
+00:01:41,950 --> 00:01:46,550
+أجمع صفات وخصائص عشان أعرفها من خلال الفحوصات
+
+25
+00:01:46,550 --> 00:01:51,510
+الكيميائية، شوف فكرة الفحوصات الكيميائية، كل بكتيريا
+
+26
+00:01:51,510 --> 00:01:55,610
+عندها إنزيمات معينة، أنا بدي أكشف هذه الإنزيمات
+
+27
+00:01:55,610 --> 00:02:00,170
+موجودة أو لا، إحنا بنعرف إن الإنزيم بيشتغل على
+
+28
+00:02:00,170 --> 00:02:05,090
+substrate، عشان أكشف هل الإنزيم موجود في البكتيريا
+
+29
+00:02:05,090 --> 00:02:10,210
+أو لا، بجيب مادة كيميائية أقلد وأحاكي فيها الـ
+
+30
+00:02:10,210 --> 00:02:13,090
+substrate اللي بيشتغل عليها الإنزيم الموجود
+
+31
+00:02:13,090 --> 00:02:17,750
+بالبكتيريا، ونستدل طبعًا إن التفاعل صار أو لا من
+
+32
+00:02:17,750 --> 00:02:22,230
+خلال نواتج التفاعل، ممكن أشوف نواتج التفاعل بالـ
+
+33
+00:02:22,230 --> 00:02:28,290
+eye، أو ممكن يكون تغير في لون الـ media، اليوم إن
+
+34
+00:02:28,290 --> 00:02:32,730
+شاء الله هنبدأ بأول فحص كيميائي، الـ catalase test
+
+35
+00:02:32,730 --> 00:02:37,530
+لكن قبل ما نتكلم عن الـ catalase and coagulase test
+
+36
+00:02:37,530 --> 00:02:46,870
+هنتكلم عن الإنزيم بشكل عام، enzymes
+
+37
+00:02:46,870 --> 00:02:50,830
+in the microbiology lab، biochemical test relies on
+
+38
+00:02:50,830 --> 00:02:55,100
+the enzymes، في معمل الـ Microbiology، الـ biochemical
+
+39
+00:02:55,100 --> 00:02:59,340
+test، الفحوصات بتعتمد على الإنزيمات، which is
+
+40
+00:02:59,340 --> 00:03:04,120
+a glycoprotein or protein that acts as a catalyst by
+
+41
+00:03:04,120 --> 00:03:07,000
+lowering the activation energy of certain
+
+42
+00:03:07,000 --> 00:03:11,660
+biological reactions، فيه تعريف مهم اللي هو الـ
+
+43
+00:03:11,660 --> 00:03:16,570
+enzyme، تعريف للإنزيم، اللي هو ممكن إنها تكون الـ
+
+44
+00:03:16,570 --> 00:03:21,590
+glycoprotein أو بروتين، يبقى الإنزيمات ممكن تكون
+
+45
+00:03:21,590 --> 00:03:27,510
+glycoprotein، بروتين مرتبط بولي سكاريد، بسكر، أو
+
+46
+00:03:27,510 --> 00:03:33,090
+بروتين، أو بروتين لحاله، بيشتغل as a catalyst، كمحفز
+
+47
+00:03:34,880 --> 00:03:39,600
+بيشتغل كمحفز، بيقلل طاقة التنشيط، lowering the
+
+48
+00:03:39,600 --> 00:03:43,760
+activation energy، طيب لو بدنا نعرف طاقة التنشيط، شو
+
+49
+00:03:43,760 --> 00:03:47,880
+هي الـ activation energy؟ طاقة التنشيط هو الحد
+
+50
+00:03:47,880 --> 00:03:52,620
+الأدنى من الطاقة اللازمة لحدوث التفاعل، يبقى أقل شيء
+
+51
+00:03:52,620 --> 00:03:56,600
+من الطاقة أنا بدي إياه عشان يصير التفاعل، بسميه الـ
+
+52
+00:03:56,600 --> 00:04:01,810
+activation energy، بالنسبة للمعادلة، قلنا الإنزيم
+
+53
+00:04:01,810 --> 00:04:05,190
+هَيشتغل على substrate، الـ substrate طبعًا هي مواد
+
+54
+00:04:05,190 --> 00:04:10,290
+التفاعل، عشان يعطيني product، نستطيع استخدام علمنا
+
+55
+00:04:10,290 --> 00:04:15,870
+في الإنزيمات البكتيرية لنعرف البكتيريا، ونقرر بين
+
+56
+00:04:15,870 --> 00:04:20,300
+مجموعات البكتيريا، هدفنا من دراسة الإنزيمات
+
+57
+00:04:20,300 --> 00:04:24,420
+بالبكتيريا، عشان أعمل identification، وأميز
+
+58
+00:04:24,420 --> 00:04:29,260
+البكتيريا عن بعض، زي ما وضحنا في بداية المحاضرة
+
+59
+00:04:29,260 --> 00:04:36,220
+types
+
+60
+00:04:36,220 --> 00:04:41,280
+of enzymes، أنواع الإنزيمات، أنواع الإنزيمات تنقسم
+
+61
+00:04:41,280 --> 00:04:47,440
+حسب، according to the site of the reaction، حسب موقع
+
+62
+00:04:47,440 --> 00:04:53,920
+العمل، وحسب مكان، أو حسب الإنتاج، يبقى بقسم الإنزيمات
+
+63
+00:04:53,920 --> 00:04:57,780
+حسب موقع العمل، الـ site of reaction، أو حسب الـ
+
+64
+00:04:57,780 --> 00:05:03,440
+enzyme production، حسب الإنتاج، بالنسبة لموقع العمل
+
+65
+00:05:03,440 --> 00:05:08,580
+الـ site of reaction، بصنفها إلى endoenzyme and
+
+66
+00:05:08,580 --> 00:05:13,860
+exoenzyme، يبقى الإنزيمات تنقسم على حسب الـ site of
+
+67
+00:05:13,860 --> 00:05:18,820
+reaction، إلى endoenzyme and exoenzyme، بالنسبة للـ
+
+68
+00:05:18,820 --> 00:05:22,840
+endoenzyme، بيصير التفاعل intracellular، يبقى
+
+69
+00:05:22,840 --> 00:05:27,980
+بيصير التفاعل داخل الخلية، where the substrate and
+
+70
+00:05:27,980 --> 00:05:33,150
+enzyme react inside the cell، بقول لي إن الـ
+
+71
+00:05:33,150 --> 00:05:36,410
+substrate والـ enzyme هيتفاعلوا داخل الخلية، الـ
+
+72
+00:05:36,410 --> 00:05:41,150
+enzyme يفرز داخل الخلية، بتفرز الخلية الـ substrate،
+
+73
+00:05:41,150 --> 00:05:45,270
+ممكن إنها تكون جوا الخلية، وممكن إنها تكون برا
+
+74
+00:05:45,270 --> 00:05:49,050
+الخلية، هلأ لو كانت الـ substrate داخل الخلية، ما في
+
+75
+00:05:49,050 --> 00:05:52,730
+عندي مشكلة، الـ enzyme يفرز من داخل الخلية، الـ
+
+76
+00:05:52,730 --> 00:05:55,410
+substrate موجودة داخل الخلية، التفاعل هيكون
+
+77
+00:05:55,410 --> 00:06:00,980
+intracellular، لكن لو كانت الـ substrate خارج الخلية
+
+78
+00:06:00,980 --> 00:06:05,620
+والإنزيم أكيد هيتم إنتاجه داخل الخلية، والإنزيم
+
+79
+00:06:05,620 --> 00:06:10,520
+endoenzyme، لازم التفاعل يصير intracellular، عشان
+
+80
+00:06:10,520 --> 00:06:15,820
+هيك، الخلية هتفرز إنزيمات، هذه الإنزيمات وظيفتها
+
+81
+00:06:15,820 --> 00:06:19,380
+أنها تدخل الـ substrate لداخل الخلية عشان يصير
+
+82
+00:06:19,380 --> 00:06:25,410
+التفاعل intracellular، يبقى بالنسبة للـ Endoenzyme،
+
+83
+00:06:25,410 --> 00:06:28,590
+الـ enzyme يفرز داخل الخلية، الـ substrate ممكن تكون
+
+84
+00:06:28,590 --> 00:06:32,230
+داخل الخلية، وممكن تكون خارج الخلية، إذا كانت داخل
+
+85
+00:06:32,230 --> 00:06:35,570
+الخلية، ما في عندي أي مشكلة، لو كانت خارج الخلية
+
+86
+00:06:35,570 --> 00:06:39,510
+وقتها الخلية هتفرز إنزيمات، هذه الإنزيمات وظيفتها
+
+87
+00:06:39,510 --> 00:06:42,370
+أنها تدخل الـ substrate لجوة الخلية، عشان يصير
+
+88
+00:06:42,370 --> 00:06:47,570
+التفاعل داخل الخلية، من الأمثلة على الـ Endoenzyme،
+
+89
+00:06:47,570 --> 00:06:53,810
+أكسيداز، كتلاز، بيورياز، نيترات ريدكتاز، طبعًا هدول حفظ
+
+90
+00:06:53,810 --> 00:06:57,630
+هنتعرف عليهم في المعامل اللي جاي، هناخد كل فحص و
+
+91
+00:06:57,630 --> 00:07:01,930
+نتعرف على الـ principle لكل فحص، وكيف بنشتغل هذا
+
+92
+00:07:01,930 --> 00:07:07,870
+الفحص، بالنسبة لـ Exoenzyme، الـ Exoenzyme، مكان حدوث
+
+93
+00:07:07,870 --> 00:07:13,670
+التفاعل خارج الخلية، extracellular، where the substrate
+
+94
+00:07:13,670 --> 00:07:18,290
+and enzyme react outside the cell، بقول الـ enzyme و
+
+95
+00:07:18,290 --> 00:07:23,330
+الـ substrate هيتفاعلوا خارج الخلية، الإنزيم برضه
+
+96
+00:07:23,330 --> 00:07:28,170
+هتفرز الخلية، هيفرز داخل الخلية، لكن الـ substrate
+
+97
+00:07:28,170 --> 00:07:32,130
+هتكون فقط خارج الخلية، إحنا قلنا في الـ endoenzyme
+
+98
+00:07:32,130 --> 00:07:36,210
+ممكن تكون داخل، وممكن تكون خارج، لكن في الـ exoenzyme
+
+99
+00:07:36,210 --> 00:07:40,990
+الـ substrate هتكون خارج الخلية، ما بقدر أدخل الـ
+
+100
+00:07:40,990 --> 00:07:43,970
+substrate، زي ما عملت في الـ Endoenzyme، لداخل
+
+101
+00:07:43,970 --> 00:07:48,290
+الخلية، لأن حجم الـ substrate كبير، عشان كده الـ
+
+102
+00:07:48,290 --> 00:07:53,490
+enzyme بيطلع لبرا، يبقى الـ Exoenzyme، الـ enzyme
+
+103
+00:07:53,490 --> 00:07:57,230
+بيفرز داخل الخلية، بيطلع لبرا عند الـ substrate، لأن
+
+104
+00:07:57,230 --> 00:08:01,570
+الـ substrate حجمها كبير، وبيصير التفاعل بين الـ
+
+105
+00:08:01,570 --> 00:08:06,130
+enzyme والـ substrate خارج الخلية، من الأمثلة على
+
+106
+00:08:06,130 --> 00:08:11,180
+الـ Exoenzyme، وحفظ، precollagenase، جيلاتيناز، أميلاز،
+
+107
+00:08:11,180 --> 00:08:16,360
+ليباز، كازيناز، يبقى هدول أمثلة على الـ Exoenzyme
+
+108
+00:08:16,360 --> 00:08:20,240
+يبقى هذا بالنسبة للـ site of reaction، قسمنا
+
+109
+00:08:20,240 --> 00:08:25,320
+الإنزيمات لإندوإنزايم and إكزوإنزايم، بالنسبة
+
+110
+00:08:25,320 --> 00:08:30,780
+للإنزايم production، موقع أو حسب الإنتاج، حسب
+
+111
+00:08:30,780 --> 00:08:34,520
+الإنتاج، بقسمه لـ inducible and constitutive
+
+112
+00:08:34,520 --> 00:08:40,300
+inducible و constitutive، شو الفرق بينهم؟ بقول لي الـ
+
+113
+00:08:40,300 --> 00:08:45,120
+inducible، بكون عندي محفز، يتم إنتاج الـ enzyme فقط
+
+114
+00:08:45,120 --> 00:08:50,260
+في وجود محفز، المحفز هيحفظ هذا الـ enzyme، produce
+
+115
+00:08:50,260 --> 00:08:56,760
+only when needed or induced، فقط عندما يكون المحفز
+
+116
+00:08:56,760 --> 00:09:02,040
+يتم إنتاج هذا الـ enzyme، لكن الـ constitutive بيتم
+
+117
+00:09:02,040 --> 00:09:07,590
+إنتاجه بشكل مستمر، continuously، بشكل مستمر بغض النظر
+
+118
+00:09:07,590 --> 00:09:13,350
+فيه عندي محفز أو لا، هذا بالنسبة لأنواع الإنزيم حسب
+
+119
+00:09:13,350 --> 00:09:17,050
+الـ enzyme production، حسب الإنتاج، يبقى type of
+
+120
+00:09:17,050 --> 00:09:20,550
+enzyme، حسب الـ site of reaction، إندو إنزيم، وإكزو
+
+121
+00:09:20,550 --> 00:09:25,610
+إنزيم، حسب الـ enzyme production، لـ inducible و
+
+122
+00:09:25,610 --> 00:09:26,690
+constitutive
+
+123
+00:09:36,140 --> 00:09:40,920
+notes، ملاحظات مهمة، every genus of bacteria has its
+
+124
+00:09:40,920 --> 00:09:44,880
+own unique set of enzymes، so we can identify it، بقول
+
+125
+00:09:44,880 --> 00:09:48,840
+لي كل مجموعة من البكتيريا إلها إنزيمات خاصة فيها،
+
+126
+00:09:48,840 --> 00:09:54,320
+هذا بيساعدني إني أنا أعمل identification للبكتيريا،
+
+127
+00:09:54,320 --> 00:09:59,300
+على سبيل المثال، الـ gram positive cocci بتضم
+
+128
+00:09:59,300 --> 00:10:04,460
+الستريبتوكوكاي cocci والستافيلوكوكاي cocci، الستافيلوكوكاي عندها
+
+129
+00:10:04,460 --> 00:10:08,720
+إنزيم الكتالاز، لكن الستريبتوكوكاي ما عندها إنزيم
+
+130
+00:10:08,720 --> 00:10:12,840
+الكتالاز، بروح أنا بشتغل فحص الكتالاز، إذا طلعت
+
+131
+00:10:12,840 --> 00:10:17,240
+catalase negative، يبقى بحكي إنه هي ستريبتوكوكاي، هي
+
+132
+00:10:17,240 --> 00:10:21,700
+قدرت أنا أعمل identification من خلال الإنزيمات، من
+
+133
+00:10:21,700 --> 00:10:26,980
+خلال وجود إنزيمات في بكتيريا معينة، وعدم وجودها في
+
+134
+00:10:26,980 --> 00:10:33,090
+بكتيريا أخرى، Endoenzyme may act outside the cell in
+
+135
+00:10:33,090 --> 00:10:36,250
+case of the presence of a high concentration of its
+
+136
+00:10:36,250 --> 00:10:41,530
+substrate، اتفقنا إنه الـ Endoenzyme بيفرز داخل
+
+137
+00:10:41,530 --> 00:10:45,690
+الخلية وبيظل داخلها، ما بيطلع برا، وبيصير التفاعل
+
+138
+00:10:45,690 --> 00:10:48,510
+داخل الخلية، لو كانت الـ substrate برا، هي اللي
+
+139
+00:10:48,510 --> 00:10:52,730
+بتدخل لجوة، فَـ هذه الجملة بقول لي في عندي استثناء،
+
+140
+00:10:52,730 --> 00:10:58,330
+إحنا قلنا إنه ما فيش عندي absolute، أكيد لكل قاعدة
+
+141
+00:10:58,330 --> 00:11:04,580
+شواذ، ممكن الـ Endoenzyme يطلع برا الخلية في حالة
+
+142
+00:11:04,580 --> 00:11:09,720
+واحدة فقط، لو كان تركيز الـ substrate خارج الخلية
+
+143
+00:11:09,720 --> 00:11:15,080
+أكثر بكثير من تركيزه في الداخل، عشان هيك الـ enzyme
+
+144
+00:11:15,080 --> 00:11:18,880
+هتفرز الخلية، ويطلع لبرا عند التركيز العالي من الـ
+
+145
+00:11:18,880 --> 00:11:23,980
+substrate، ويصير التفاعل، هذا بالنسبة للملاحظة
+
+146
+00:11:23,980 --> 00:11:29,840
+الثانية، الـ Endoenzyme ممكن إنه يطلع خارج الخلية في
+
+147
+00:11:29,840 --> 00:11:33,120
+حال كان عندي الـ concentration of its substrate
+
+148
+00:11:33,120 --> 00:11:38,440
+تركيزه خارج الخلية أعلى من داخل الخلية، آخر ملاحظة،
+
+149
+00:11:38,440 --> 00:11:42,840
+the same enzyme could be inducible and constitutive
+
+150
+00:11:42,840 --> 00:11:48,320
+in different genera، كاتب مثال اليورياز، إحنا قلنا
+
+151
+00:11:48,320 --> 00:11:52,800
+إن الإنزيم يقسم حسب الإنتاج إلى inducible and
+
+152
+00:11:52,800 --> 00:11:59,140
+constitutive، هذه الجملة تقول إن الإنزيم نفسه، مثلًا X،
+
+153
+00:11:59,140 --> 00:12:03,640
+ممكن يكون inducible في بكتيريا معينة، و constitutive
+
+154
+00:12:03,640 --> 00:12:08,340
+في بكتيريا أخرى، من الأمثلة على هذا الإنزيم، اللي
+
+155
+00:12:08,340 --> 00:12:12,000
+ممكن يكون inducible في بكتيريا، و constitutive في
+
+156
+00:12:12,000 --> 00:12:16,960
+بكتيريا أخرى، اليورياز، اليورياز من اسمه، يورياز
+
+157
+00:12:16,960 --> 00:12:22,230
+هيكسر اليوريا، اليوريا طبعًا هي toxic waste product،
+
+158
+00:12:22,230 --> 00:12:27,010
+نتخلص منها عن طريق تكسيرها لـ ammonia and CO2،
+
+159
+00:12:27,010 --> 00:12:34,910
+بالنسبة لليورياز، هيكسر لي اليوريا لـ ammonia and
+
+160
+00:12:34,910 --> 00:12:43,190
+CO2، فلو كان بالنسبة للـ inducible، في بكتيريا اسمها
+
+161
+00:12:43,190 --> 00:12:48,370
+Proteus، بتفرز إنزيم اليورياز في وجود محفز، يعني
+
+162
+00:12:49,580 --> 00:12:53,320
+بنعتبرها inducible، لما نعتبر الإنزيم حين
+
+163
+00:12:53,320 --> 00:12:58,080
+inducible، لكن في حال عدم وجود المحفز لا يتم إنتاجه،
+
+164
+00:12:58,080 --> 00:13:02,360
+يبقى حين كان الإنزيم اليورياز inducible في
+
+165
+00:13:02,360 --> 00:13:07,740
+البروتياس فقط في وجود محفز هيتم إنتاجه، لكن فيه
+
+166
+00:13:07,740 --> 00:13:12,400
+بكتيريا، الـ Helicobacter pylori، يتم إنتاجه بشكل
+
+167
+00:13:12,400 --> 00:13:18,610
+مستمر، يعني يعتبر constitutive، الـ H. pylori بتكون
+
+168
+00:13:18,610 --> 00:13:23,030
+في المعدة، طريقة الـ infection، تقليل حموضة المعدة،
+
+169
+00:13:23,030 --> 00:13:28,450
+كيف؟ بتفرز إنزيم اليورياز، عشان تتكسر اليوريا،
+
+170
+00:13:28,450 --> 00:13:33,210
+وتنتج الأمونيا، الأمونيا طبعًا قاعدة، هيرتفع الـ pH،
+
+171
+00:13:33,210 --> 00:13:38,490
+وتقلل من حموضة المعدة، يبقى الإنزيم نفسه، اليورياز،
+
+172
+00:13:38,490 --> 00:13:43,390
+ممكن يكون inducible، وممكن يكون constitutive، ذكرنا
+
+173
+00:13:43,390 --> 00:13:49,400
+مثل اليورياز إنز
+
+223
+00:17:37,500 --> 00:17:44,240
+يُعتبر إله نوعين أو لإله هدفين إن أنا أتخلص من الـ
+
+224
+00:17:44,240 --> 00:17:48,610
+toxic West اللي هي اليوريا، وبنفس الوقت بدي أحصل
+
+225
+00:17:48,610 --> 00:17:53,050
+على non-carbohydrate carbon source، يبقى اليورياز
+
+226
+00:17:53,050 --> 00:17:58,310
+كان في النوع الأول والنوع الرابع، خلصنا الـ
+
+227
+00:17:58,310 --> 00:18:04,410
+enzymatic reaction. هلأ هنبدأ في أول نوع أو أول
+
+228
+00:18:04,410 --> 00:18:10,810
+enzyme اللي هو الكتالاز. اسم الـ enzyme طبعاً من اسم
+
+229
+00:18:10,810 --> 00:18:15,290
+الفحص اللي هو الكتالاز. الـ substrate اللي هيشتغل
+
+230
+00:18:15,290 --> 00:18:19,790
+عليها الـ enzyme، الـ hydrogen peroxide، الـ H2O2،
+
+231
+00:18:19,790 --> 00:18:25,270
+اللي قلنا هي toxic material. الـ enzyme action
+
+232
+00:18:25,270 --> 00:18:30,850
+breaks down the toxic H2O2, producing oxygen gas
+
+233
+00:18:30,850 --> 00:18:36,230
+and water. بيقول لي هيكسر الـ toxic H2O2. قلنا إن الـ
+
+234
+00:18:36,230 --> 00:18:40,530
+H2O2، الـ hydrogen peroxide، يعتبر toxic waste
+
+235
+00:18:40,530 --> 00:18:46,150
+product. هيتكسر لـ O2 gas and water. المعادلة واضحة،
+
+236
+00:18:46,150 --> 00:18:50,050
+تفاعل الـ H2O2. قلنا الـ substrate،  الـ enzyme الـ
+
+237
+00:18:50,050 --> 00:18:54,650
+catalase. نواتج التفاعل O2 gas and water. الـ
+
+238
+00:18:54,650 --> 00:19:00,550
+H2O2. قلنا من أنواع الـ enzymatic reaction إنه يكسر
+
+239
+00:19:00,550 --> 00:19:05,110
+الـ worst product، الناتج من العمليات الأيضية. الـ
+
+240
+00:19:05,110 --> 00:19:10,830
+catalase هيكسر الـ H2O2 الناتج من عملية التنفس
+
+241
+00:19:10,830 --> 00:19:17,090
+الخلوي، الـ aerobic respiration. يبقى الـ H2O2 هيتم
+
+242
+00:19:17,090 --> 00:19:20,750
+إنتاجه خلال عملية التنفس الخلوي، الـ aerobic
+
+243
+00:19:20,750 --> 00:19:26,630
+respiration. كيف بيتم إنتاج الـ H2O2 في عملية
+
+244
+00:19:26,630 --> 00:19:32,010
+التنفس الخلوي؟ في المرحلة electron transport chain.
+
+245
+00:19:38,180 --> 00:19:41,240
+في عندي إلكترونات، electron
+
+246
+00:20:08,540 --> 00:20:13,180
+في عندنا electron transport chain، بيتم انتقال
+
+247
+00:20:13,180 --> 00:20:17,340
+الإلكترون من جزء لجزء حتى يوصل للمستقبل النهائي
+
+248
+00:20:17,340 --> 00:20:21,420
+للإلكترونات. المستقبل النهائي للإلكترونات هو
+
+249
+00:20:21,420 --> 00:20:27,680
+الأكسجين. هيرتبط الإلكترون بالمدار الأخير للأكسجين،
+
+250
+00:20:27,680 --> 00:20:30,260
+حسب المعادلة التالية.
+
+251
+00:20:41,340 --> 00:20:55,740
+طبعاً هذا الـ oxygen، يبقى
+
+252
+00:20:55,740 --> 00:20:59,280
+هيرتبط الـ electron خلال انتقاله في الـ electronic
+
+253
+00:20:59,280 --> 00:21:03,460
+transport، لكي ينتقل به للمستقبل أو هيرتبط به
+
+254
+00:21:03,460 --> 00:21:07,120
+المستقبل النهائي للإلكترونات اللي هو الـ oxygen، هو
+
+255
+00:21:07,120 --> 00:21:09,840
+هيعطيني الـ superoxide.
+
+256
+00:21:29,030 --> 00:21:38,570
+هيعطيني الـ superoxide. هذا بسميه الـ
+
+257
+00:21:38,570 --> 00:21:47,030
+super
+
+258
+00:21:47,030 --> 00:21:58,170
+oxide أو الـ free radical، ممكن نسميه هذا
+
+259
+00:21:58,170 --> 00:22:01,870
+الـ superoxide أو الـ free radical يعتبر طبعاً
+
+260
+00:22:01,870 --> 00:22:07,150
+toxic، unstable. طبعاً هنتخلص منه. في عندي في الـ
+
+261
+00:22:07,150 --> 00:22:12,070
+electronic transport chain، في عندي مضخات لـ H+.
+
+262
+00:22:12,070 --> 00:22:16,550
+تمام. الـ H+، فبالتالي الـ superoxide هذا
+
+263
+00:22:16,550 --> 00:22:22,270
+هيرتبط بالـ H+ ويعطيني الـ hydrogen peroxide.
+
+264
+00:22:22,270 --> 00:22:23,850
+نكمل المعادلة.
+
+265
+00:22:33,320 --> 00:22:37,780
+الـ superoxide هذا unstable، free radical، toxic،
+
+266
+00:22:37,780 --> 00:22:42,040
+يعتبر. عشان أتخلص منه، خلال أو موجود فيه الـ
+
+267
+00:22:42,040 --> 00:22:51,540
+electronic transport chain، في عندي الـ H+، الـ
+
+268
+00:22:51,540 --> 00:22:56,260
+H+ بواسطة إنزيم الـ superoxide dismutase،
+
+269
+00:22:56,260 --> 00:23:00,980
+هيتحول لـ H2O2، اللي هو الـ hydrogen peroxide.
+
+270
+00:23:39,680 --> 00:23:45,400
+بواسطة إنزيم السوبرأوكسايد دسميوتاز هيتحول لـ H2O
+
+271
+00:23:45,400 --> 00:23:54,740
+اللي
+
+272
+00:23:54,740 --> 00:24:00,020
+هو الهيدروجين بيروكسايد، طبعاً
+
+273
+00:24:00,020 --> 00:24:03,680
+ممكن نوزن المعادلة، اللي هي هتحتاج اثنين
+
+274
+00:24:11,990 --> 00:24:17,130
+هكذا. هذا هو الهيدروجين بيروكسايد، هيك اتكون
+
+275
+00:24:17,130 --> 00:24:22,870
+الهيدروجين بيروكسايد، هيتكسر لـ gas and water بواسطة
+
+276
+00:24:22,870 --> 00:24:25,970
+إنزيم الكتالاز. هنتخلص من الهيدروجين بيروكسايد، يعتبر
+
+277
+00:24:25,970 --> 00:24:31,050
+toxic للـ DNA وللـ cell membrane، عشان نتخلص منه،
+
+278
+00:24:31,050 --> 00:24:35,850
+هيتم إفراز إنزيم الكتالاز. الكتالاز هيكسر لي الـ O2
+
+279
+00:24:35,850 --> 00:24:41,910
+and H2O، لـ water. يبقى في الـ electron transport chain،
+
+280
+00:24:41,910 --> 00:24:46,690
+في عندي الإلكترونات، هتنتقل من جزء للجزء حتى تصل
+
+281
+00:24:46,690 --> 00:24:50,210
+للمستقبل النهائي اللي هو الـ oxygen. هترتبط بالـ
+
+282
+00:24:50,210 --> 00:24:54,050
+oxygen في المدار الأخير، هتعطيني الـ superoxide،
+
+283
+00:24:54,050 --> 00:25:00,250
+free radical، O2-. هذا الـ free radical،
+
+284
+00:25:00,250 --> 00:25:05,430
+unstable. هيرتبط بالـ H+.  هاي الـ H+ موجود
+
+285
+00:25:05,430 --> 00:25:08,550
+طبعاً فيه، عندي مضخات في الـ electron transport
+
+286
+00:25:08,550 --> 00:25:11,510
+chain، اللي هترتبط فيها بواسطة إنزيم الـ super
+
+287
+00:25:11,510 --> 00:25:16,070
+oxide dismutase. هيعطيني الـ hydrogen peroxide. هذا
+
+288
+00:25:16,070 --> 00:25:21,030
+بالنسبة لكيف تم إنتاج الـ H2O2 من عملية التنفس
+
+289
+00:25:21,030 --> 00:25:21,870
+الخلوي.
+
+290
+00:25:25,820 --> 00:25:30,580
+بيقول لي إن الـ hydrogen peroxide طبعاً لازم يتكسر
+
+291
+00:25:30,580 --> 00:25:35,240
+to prevent its toxic
+
+292
+00:25:35,240 --> 00:25:39,580
+action، عشان أمنع الـ toxic action on DNA and cell
+
+293
+00:25:39,580 --> 00:25:44,200
+membrane. قلنا هو toxic للـ DNA and cell membrane.
+
+294
+00:25:51,590 --> 00:25:55,170
+طيب، كيف بدي أكشف هل البكتيريا عندها إنزيم
+
+295
+00:25:55,170 --> 00:25:59,930
+الكتالاز أو لأ؟ قلنا بنجيب مادة فيها الـ
+
+296
+00:25:59,930 --> 00:26:03,290
+substrate اللي بيشتغل عليها الـ enzyme. إحنا قلنا إن
+
+297
+00:26:03,290 --> 00:26:07,970
+الـ substrate اللي بيشتغل عليها الكتالاز هي الـ H2O2،
+
+298
+00:26:07,970 --> 00:26:11,910
+الـ hydrogen peroxide. بنجيب مادة كيميائية من
+
+299
+00:26:11,910 --> 00:26:18,510
+المختبر اللي هي الـ hydrogen peroxide 3%، H2O2. بنضيف
+
+300
+00:26:18,510 --> 00:26:23,390
+عليها كولوني. هذه الكولوني طبعاً إذا كانت الكتالاز
+
+301
+00:26:23,390 --> 00:26:28,170
+البكتيريا موجود فيها إنزيم الكتالاز، هيتكسر بواسطة
+
+302
+00:26:28,170 --> 00:26:33,090
+الانزيم، وينتج oxygen. الـ oxygen بشوفه على شكل air
+
+303
+00:26:33,090 --> 00:26:36,590
+bubble. يبقى when hydrogen peroxide is added to a
+
+304
+00:26:36,590 --> 00:26:39,630
+colony of catalase-producing bacteria، لو أنا ضفت الـ
+
+305
+00:26:39,630 --> 00:26:44,820
+hydrogen peroxide، الـ hydrogen peroxide اللي هو الـ
+
+306
+00:26:44,820 --> 00:26:49,700
+substrate 3% H2O2، وضفته للكولوني للبكتيريا اللي
+
+307
+00:26:49,700 --> 00:26:54,580
+بتفرز الكتالاز، أكيد هيتكسر لأكسجين، الأكسجين بقدر
+
+308
+00:26:54,580 --> 00:26:57,580
+أني أنا أكشف عنه من خلال إني أنا بشوفه على شكل
+
+309
+00:26:57,580 --> 00:27:02,320
+bubbles. significance، significance، هتمر معناها
+
+310
+00:27:02,320 --> 00:27:05,920
+كتير، كل فحص كيميائي لازم يكون له significance،
+
+311
+00:27:05,920 --> 00:27:10,190
+أو لازم نعرف شو الـ significance لهذا الفحص. الـ
+
+312
+00:27:10,190 --> 00:27:14,170
+significance يعني شو أهمية الفحص؟ بيفرق بين مين
+
+313
+00:27:14,170 --> 00:27:18,050
+ومين؟ مين الـ positive ومين الـ negative لهذا الفحص؟
+
+314
+00:27:18,050 --> 00:27:23,930
+قبل ما نتكلم على هاي الـ slide، بدنا نرجع نبدأ من
+
+315
+00:27:23,930 --> 00:27:33,790
+الـ Gram stain. لو أنا عملت، نرسم diagram، عملت
+
+316
+00:27:33,790 --> 00:27:34,970
+Gram stain.
+
+317
+00:27:53,530 --> 00:27:56,590
+قلنا الـ Gram stain بتقسم البكتيريا لـ Gram
+
+318
+00:27:56,590 --> 00:27:58,070
+positive و Gram negative.
+
+319
+00:28:35,260 --> 00:28:39,080
+يبقى الـ Gram stain بتقسم البكتيريا لـ Gram positive و Gram
+
+320
+00:28:39,080 --> 00:28:42,380
+negative. كمان Gram positive و Gram negative
+
+321
+00:28:42,380 --> 00:28:45,980
+تتقسم، لأنها تصبغ  بإيجابي وسلبي.
+
+322
+00:29:24,040 --> 00:29:41,880
+Okay، بس الـ line بس
+
+323
+00:29:41,880 --> 00:29:42,260
+الـ line
+
+324
+00:29:54,170 --> 00:29:57,930
+يبقى بالنهاية قسمت لي إلى Gram positive cocci، Gram
+
+325
+00:29:57,930 --> 00:30:01,350
+positive bacilli، Gram negative cocci، Gram negative
+
+326
+00:30:01,350 --> 00:30:06,470
+bacilli. قلنا إن كل مجموعة بتضم مثلاً Gram positive
+
+327
+00:30:06,470 --> 00:30:10,130
+cocci بتضم مجموعة من البكتيريا. Gram positive bacilli
+
+328
+00:30:10,130 --> 00:30:13,570
+بتضم مجموعة من البكتيريا. كيف بدي أفرق بينهم؟
+
+329
+00:30:13,570 --> 00:30:17,980
+باستخدام الـ biochemical tests.  Gram positive bacteria
+
+330
+00:30:17,980 --> 00:30:21,980
+بتضم الـ streptococci، الـ staphylococci، الـ macro-
+
+331
+00:30:21,980 --> 00:30:28,020
+cocci، والـ micrococci. بيقول لي عشان أفرق بين هذول
+
+332
+00:30:28,020 --> 00:30:33,980
+المجموعات، بعمل فحص الكتالاز. طبعاً، لو طلعت عملت
+
+333
+00:30:33,980 --> 00:30:37,280
+Gram stain وطلعت معايا Gram positive cocci، على طول
+
+334
+00:30:37,280 --> 00:30:43,150
+بعمل فحص الكتالاز عشان أفرق بين الـ streptococci
+
+335
+00:30:43,150 --> 00:30:46,790
+وباقي الأنواع، الـ staphylo، والـ macro، والـ micro. يبقى
+
+336
+00:30:46,790 --> 00:30:49,890
+الخطوة الثانية إني أنا بدي أعمل الـ catalase test.
+
+337
+00:31:24,380 --> 00:31:39,100
+الـ catalase test. لو
+
+338
+00:31:39,100 --> 00:31:41,780
+عملت الـ catalase test وطلع negative،
+
+339
+00:31:57,390 --> 00:32:00,230
+يبقى البكتيريا هي streptococcal.
+
+340
+00:32:45,870 --> 00:32:49,830
+هذا بالنسبة لـ catalase test. لو طلعت عملت الفحص و
+
+341
+00:32:49,830 --> 00:32:53,910
+طلعت negative، يبقى هي streptococci. لكن لو طلعت
+
+342
+00:32:53,910 --> 00:33:03,410
+positive، في عندي ثلاث احتمالات: إما staphylococci،
+
+343
+00:33:03,410 --> 00:33:13,670
+staphylococci، أو macrococci، أو micrococci، أو
+
+344
+00:33:13,670 --> 00:33:14,270
+micro
+
+345
+00:33:28,760 --> 00:33:34,220
+Micrococci، Staphylococci، Macrococci،
+
+346
+00:33:34,220 --> 00:33:38,000
+Micrococci. يبقى هي positive. يبقى فحص الكتالاز فرق
+
+347
+00:33:38,000 --> 00:33:41,060
+اللي بين الـ streptococci وما بين الـ staphylo-
+
+348
+00:33:41,060 --> 00:33:46,130
+والـ macro والـ micrococci. الـ Gram positive bacilli
+
+349
+00:33:46,130 --> 00:33:52,390
+برضه بتضم الـ bacillus والـ clostridium. الـ bacillus لو أنا عملت
+
+350
+00:33:52,390 --> 00:33:57,270
+فحص الكتالاز، الـ bacillus هو positive، والـ clostridium
+
+351
+00:33:57,270 --> 00:34:01,570
+negative. تمام. وفي عندي كمان الـ Gram negative
+
+352
+00:34:01,570 --> 00:34:08,950
+bacilli بتضم الـ listeria monocytogenes. يبقى الـ Gram
+
+353
+00:34:08,950 --> 00:34:12,790
+positive bacilli، في عندي، أنا بعمل فحص الكتالاز، نفس
+
+354
+00:34:12,790 --> 00:34:13,310
+الفكرة.
+
+355
+00:34:43,130 --> 00:34:46,030
+يبقى الـ Gram positive bacilli بتضم الـ bacillus،
+
+356
+00:34:46,030 --> 00:34:50,230
+Clostridium، والـ listeria monocytogenes. عشان أفرق
+
+357
+00:34:50,230 --> 00:34:55,110
+بينهم بعمل فحص الكتالاز. لو طلعت catalase positive، يبقى
+
+358
+00:34:55,110 --> 00:34:58,530
+في عندي احتمال إما إنها تكون bacillus أو الـ listeria
+
+359
+00:34:58,530 --> 00:35:03,010
+monocytogenes. لو كانت negative، يبقى هي Clostridium.
+
+360
+00:35:03,010 --> 00:35:07,350
+يبقى هي بتكون Clostridium. عندي أو Clostridium أو
+
+361
+00:35:07,350 --> 00:35:11,280
+الـ listeria monocytogenes. بعمل فحص الكتالاز، لو طلعت
+
+362
+00:35:11,280 --> 00:35:28,860
+positive أو طلعت negative. لو كانت negative، لو
+
+363
+00:35:28,860 --> 00:35:31,620
+كانت negative، يبقى هي Clostridium.
+
+364
+00:36:06,780 --> 00:36:12,780
+لكن لو كانت positive، في عندي احتمالين، إما
+
+365
+00:36:12,780 --> 00:36:19,400
+إنها تكون Bacillus، أو
+
+366
+00:36:19,400 --> 00:36:25,440
+إنها تكون Listeria monocytogenes. Bacillus
+
+367
+00:36:25,440 --> 00:36:30,580
+أو Listeria
+
+368
+00:36:30,580 --> 00:36:35,600
+mono
+
+369
+00:36:44,510 --> 00:36:51,410
+cytogenes. يبقى الـ bacillus والـ listeria monocytogenes، catalase
+
+370
+00:36:51,410 --> 00:36:59,530
+positive. كيف
+
+371
+00:36:59,530 --> 00:37:03,250
+نفرق بين الـ staphylo، والـ macro، والـ micro؟ هنتعرف عليها
+
+372
+00:37:03,250 --> 00:37:07,410
+في المحاضرات الجاية. لكن بالنسبة للـ bacillus والـ listeria mono
+
+373
+00:37:07,410 --> 00:37:11,420
+cytogenes، لو أنا بدي أفرق بينهم، بفحص اللي هو الـ
+
+374
+00:37:11,420 --> 00:37:32,820
+Spore stain. Spore stain. Spore stain.
+
+375
+00:37:34,310 --> 00:37:38,230
+بعمله، لو طلعت spore forming، يبقى هي الـ bacillus،
+
+376
+00:37:38,230 --> 00:37:42,390
+لكن لو طلعت non spore forming، يبقى هي الـ listeria
+
+377
+00:37:42,390 --> 00:37:46,750
+monocytogenes. هيك أنا قدرت أبدأ أتعرف على
+
+378
+00:37:46,750 --> 00:37:49,870
+البكتيريا. يبقى لو عملت Gram stain، طلعت Gram
+
+379
+00:37:49,870 --> 00:37:53,840
+positive، في عندي احتمال streptococci، staphylo-
+
+380
+00:37:53,840 --> 00:37:56,780
+cocci، macrococci، micrococci. كيف بدي أفرق
+
+381
+00:37:56,780 --> 00:37:59,700
+بينهم؟ بعمل فحص الـ catalase. لو طلعت negative، يبقى
+
+382
+00:37:59,700 --> 00:38:02,880
+هي streptococci. لو طلعت positive، في عندي ثلاث
+
+383
+00:38:02,880 --> 00:38:06,260
+احتمالات. كيف أفرق بينهم؟ وهنتعرف في المحاضرات اللي
+
+384
+00:38:06,260 --> 00:38:10,640
+جاية في الفحوصات اللي هتمر معنا. لو عملت Gram stain، و
+
+385
+00:38:10,640 --> 00:38:14,020
+طلعت Gram positive bacilli، في عندي Gram positive
+
+386
+00:38:14,020 --> 00:38:17,380
+bacilli بتضم الـ clostridium، الـ bacillus، الـ listeria mono-
+
+387
+00:38:17,380 --> 00:38:20,900
+cytogenes. كيف أفرق بينهم؟ بعمل فحص الكتالاز. لو
+
+388
+00:38:20,900 --> 00:38:24,140
+طلعت negative، يبقى هي clostridium. لو طلعت
+
+389
+00:38:24,140 --> 00:38:27,980
+positive، يبقى عندي احتمالين: إما الـ bacillus أو الـ listeria
+
+390
+00:38:27,980 --> 00:38:31,040
+monocytogenes. كيف أفرق بينهم؟ هذه المعلومة
+
+391
+00:38:31,040 --> 00:38:35,560
+أخذناها في المحاضرات السابقة. من خلال الـ bacillus،
+
+392
+00:38:35,560 --> 00:38:39,000
+تعتبر spore forming. لكن الـ listeria monocytogenes
+
+393
+00:38:39,000 --> 00:38:44,840
+تعتبر non spore forming. لـ Gram negative cocci، في عندي
+
+394
+00:38:44,840 --> 00:38:48,020
+الـ neisseria، والموروكسلا، اللي هنتعرف عليهم برضه في
+
+395
+00:38:48,020 --> 00:38:51,160
+المحاضرات اللي جاية. لـ Gram negative bacilli، في عندي الـ
+
+396
+00:38:51,160 --> 00:38:55,500
+enterobacteriaceae، و الـ pseudomonas. هدول برضه catalase
+
+397
+00:38:55,500 --> 00:38:57,880
+كلاهما catalase positive. بيبقى هذا الفحص، أنا
+
+398
+00:38:57,880 --> 00:39:02,220
+ما بستخدمه للتفريق. هناخد ف
+
+445
+00:42:42,570 --> 00:42:47,690
+24 ساعة بلاحظ نمو البكتيريا، بقول لك بتضيف 1 مل من
+
+446
+00:42:47,690 --> 00:42:52,390
+الـ reagent، اللي هو 3% hydrogen peroxide، على اللي
+
+447
+00:42:52,390 --> 00:42:57,610
+هو على سطح الإسلان، تمام؟ بعدها بشوف النتيجة كيف الـ
+
+448
+00:42:57,610 --> 00:43:00,950
+result، بتكون الـ result هنشوفها في الـ slide اللي
+
+449
+00:43:00,950 --> 00:43:07,270
+بعد النتيجة، لو كانت النتيجة positive، هذا بالنسبة للـ
+
+450
+00:43:07,270 --> 00:43:11,890
+slide method، سيشوف النتيجة الـ positive، هتكون على
+
+451
+00:43:11,890 --> 00:43:15,570
+شكل air bubble، يبقى لو اتكون عندي air bubble، يبقى
+
+452
+00:43:15,570 --> 00:43:19,270
+هي positive catalase، لكن لو ما اتكون شيء يبقى هي
+
+453
+00:43:19,270 --> 00:43:22,630
+negative catalase، أو بنحكي smooth suspension بيكون
+
+454
+00:43:22,630 --> 00:43:25,490
+الـ suspension smooth، مافي عندي أي شيء، مافي عندي
+
+455
+00:43:25,490 --> 00:43:29,830
+air bubble، هذا بالنسبة لـ slide method، لـ tube
+
+456
+00:43:29,830 --> 00:43:34,210
+method، نفس الفكرة، بعد ما أضيف الـ 1 مل من الـ
+
+457
+00:43:34,210 --> 00:43:37,470
+hydrogen peroxide، بقول لك بتكون air bubbles في الـ
+
+458
+00:43:37,470 --> 00:43:40,130
+tube، زي ما احنا ملاحظين، يبقى هي high positive
+
+459
+00:43:40,130 --> 00:43:44,210
+catalase، لكن هون ما نلاحظ، في عندي أي فقاعات، يبقى
+
+460
+00:43:44,210 --> 00:43:49,580
+هنا نفس الفكرة للسلايد ميثود، نفس الفكرة، في عندي air
+
+461
+00:43:49,580 --> 00:43:53,200
+bubbles، يبقى catalase positive، لكن هون في عندي smooth
+
+462
+00:43:53,200 --> 00:43:59,720
+suspension، يبقى هنا negative للـ catalase، هيك اللي هي الـ
+
+463
+00:43:59,720 --> 00:44:00,100
+result
+
+464
+00:44:19,840 --> 00:44:24,560
+هذه الـ slide، هنتكلم عن بعض الملاحظات المهمة، بقول
+
+465
+00:44:24,560 --> 00:44:27,860
+لي be careful when using bacteria from blood agar
+
+466
+00:44:27,860 --> 00:44:30,860
+culture and avoid touching the agar by loop
+
+467
+00:44:30,860 --> 00:44:34,780
+because blood cells in agar also had catalase
+
+468
+00:44:34,780 --> 00:44:39,160
+enzyme، بيعطيني false positive، بقول لي ممنوع آخذ
+
+469
+00:44:39,160 --> 00:44:42,620
+كولوني عشان أعمل فحص اللي هو الـ catalase test من
+
+470
+00:44:42,620 --> 00:44:47,540
+blood agar، قلنا الـ blood agar هي enriched media، منضيف
+
+471
+00:44:47,540 --> 00:44:52,240
+الـ blood اللي هو supplement، يعتبر بنسبة 5%، يعني لو
+
+472
+00:44:52,240 --> 00:44:56,400
+أنا حضرت 1 liter من الـ nutrient agar، بضيف 50 ml من
+
+473
+00:44:56,400 --> 00:44:58,900
+الـ blood، طبعاً بعد ما أعقم الـ media، و تطلع من الـ
+
+474
+00:44:58,900 --> 00:45:02,920
+autoclave، بستنى تبرد بعدين بضيف الـ blood عشان ما
+
+475
+00:45:02,920 --> 00:45:07,880
+يتكسر، طيب ليش أنا ممنوع آخذ كولوني من الـ blood agar؟
+
+476
+00:45:08,150 --> 00:45:11,930
+بقول لك لو كنت زرعت بكتيريا على blood agar، وعملتي
+
+477
+00:45:11,930 --> 00:45:15,970
+فحص الـ Catalase، ممكن يعطيني false positive result
+
+478
+00:45:15,970 --> 00:45:20,830
+ليش؟ لأنك لما تأخذي كولوني من blood agar، ممكن تروحي
+
+479
+00:45:20,830 --> 00:45:25,350
+تأخذي مع الـ colony، تجرحي الـ agar، يعني تأخذي شوية
+
+480
+00:45:25,350 --> 00:45:30,690
+media، يعني blood، قلنا الـ blood cells نفسها بتحتوي
+
+481
+00:45:30,690 --> 00:45:35,610
+على Catalase enzyme، بالتالي ممكن البكتيريا ما يكون
+
+482
+00:45:35,610 --> 00:45:39,450
+عندها أنزيم الـ Catalase، لكن أنتِ أخذتي كلّه عن طريق
+
+483
+00:45:39,450 --> 00:45:43,470
+أو من طبق الـ blood agar، وجرحتي هذا الـ agar، يعني
+
+484
+00:45:43,470 --> 00:45:47,050
+أخذتي blood اللي موجود فيه Catalase enzyme، بالتالي
+
+485
+00:45:47,050 --> 00:45:51,910
+هيتفاعل مع الـ substrate اللي احنا حاطينه، الـ H2O2
+
+486
+00:45:51,910 --> 00:45:56,710
+وبيعطيني positive result، عشان هيك أو بيعطيني false
+
+487
+00:45:56,710 --> 00:46:00,550
+positive result، نتيجة خاطئة، مش البكتيريا عندها الـ
+
+488
+00:46:00,550 --> 00:46:03,670
+enzyme، عشان هيك الأفضل إني أنا أشتغل أي فحص
+
+489
+00:46:03,670 --> 00:46:07,550
+كيميائي على general media، على nutrient agar، لو
+
+490
+00:46:07,550 --> 00:46:11,830
+اضطريت إني أنا آخذ من blood agar، بحاول إني أنا
+
+491
+00:46:11,830 --> 00:46:16,010
+آخذ بدون ما أجرح الـ media، avoid touching the agar
+
+492
+00:46:16,010 --> 00:46:20,520
+by the loop، هذا بالنسبة للملاحظة الأولى، الملاحظة
+
+493
+00:46:20,520 --> 00:46:23,480
+الثانية، also don't use bacteria from old culture
+
+494
+00:46:23,480 --> 00:46:27,320
+because the enzyme activity drop by time، بيعطيني
+
+495
+00:46:27,320 --> 00:46:31,160
+false negative، أي فحص كيميائي لازم إني أنا أشتغله
+
+496
+00:46:31,160 --> 00:46:35,360
+تكون الـ culture fresh، لأنه لو كانت old culture
+
+497
+00:46:35,360 --> 00:46:39,060
+بتكون البكتيريا منهكة، تمام؟ الـ enzyme activity
+
+498
+00:46:39,060 --> 00:46:44,770
+بيبدأ يقل، فبالتالي بيعطيني false negative result، آخر
+
+499
+00:46:44,770 --> 00:46:48,490
+ملاحظة إحنا هنضيفها، وإحنا نحكيها قبل ما نختم بالـ
+
+500
+00:46:48,490 --> 00:46:52,750
+Catalase Test، هلأ مش كل البكتيريا قلنا عندها
+
+501
+00:46:52,750 --> 00:46:54,990
+enzyme الـ Catalase، على سبيل المثال قلنا الـ
+
+502
+00:46:54,990 --> 00:46:58,770
+Streptococci ما عندها enzyme الـ Catalase، طب قلنا
+
+503
+00:46:58,770 --> 00:47:02,910
+خلال التنفس الخلوي بيتم إنتاج الـ H2O2، اللي هو
+
+504
+00:47:02,910 --> 00:47:07,690
+يعتبر toxic، waste product، كيف البكتيريا اللي ما
+
+505
+00:47:07,690 --> 00:47:11,570
+عندها enzyme الـ Catalase، هتتخلص من الـ H2O2 اللي
+
+506
+00:47:11,570 --> 00:47:14,680
+يعتبر toxic، وقلنا إن البكتيريا اللي معاها، عندها
+
+507
+00:47:14,680 --> 00:47:18,300
+إنزيم الـ catalase، عندها إنزيم آخر بيقوم بنفس
+
+508
+00:47:18,300 --> 00:47:23,240
+الوظيفة، اللي هو اسمه hydrogen peroxidase، من اسمه
+
+509
+00:47:23,240 --> 00:47:25,740
+hydrogen peroxidase، يعني بيكسر الـ hydrogen
+
+510
+00:47:25,740 --> 00:47:29,580
+peroxide، هيشتغل على نصف الـ substrate اللي هي الـ
+
+511
+00:47:29,580 --> 00:47:35,780
+H2O2، لكن هيعطيني جزيئان ماء، هيعطيني two جزيئان
+
+512
+00:47:35,780 --> 00:47:40,780
+H2O، النواتج هتختلف، هيعطيني جزيئان ماء، مش هيعطيني
+
+513
+00:47:40,780 --> 00:47:45,320
+O2، فبالتالي مش هقدر أنا أستدل على التفاعل هذا زي
+
+514
+00:47:45,320 --> 00:47:51,260
+الـ Catalase Test، هذا بالنسبة لفحص Catalase Test
+
+515
+00:47:51,260 --> 00:47:54,520
+خلصناه، هنتقل لـ Coagulase Test
diff --git a/PL9fwy3NUQKwZWgAb_V1ATlRRsH-6i3cPC/YZtUaTNI_xw_raw.json b/PL9fwy3NUQKwZWgAb_V1ATlRRsH-6i3cPC/YZtUaTNI_xw_raw.json
new file mode 100644
index 0000000000000000000000000000000000000000..6544edb2636b5cd975e102cde02e253f6eac32c3
--- /dev/null
+++ b/PL9fwy3NUQKwZWgAb_V1ATlRRsH-6i3cPC/YZtUaTNI_xw_raw.json
@@ -0,0 +1 @@
+{"segments": [{"id": 1, "seek": 3144, "start": 1.96, "end": 31.44, "text": "السلام عليكم ورحمة الله وبركاته يعطيكم العافية جميعًا في هذا اللقاء هنتكلم عن Catalase and Coagulase Test قولنا إن البكتيريا Small and Colorless اتغلبنا على إنها Small باستخدام الميكروسكوب الميكروسكوب عملّي magnification للبكتيريا وإقدرت إني أنا أشوفها بالنسبة لإنها Colorless شفافة حلّت هذه المشكلة باستخدام الستان باستخدام الصبغاط", "tokens": [6027, 3794, 37440, 25894, 24793, 4032, 2288, 35571, 3660, 21984, 4032, 26890, 4117, 9307, 3224, 37495, 9566, 1829, 24793, 18863, 31845, 10632, 10874, 2304, 40228, 14111, 995, 8978, 23758, 13672, 4587, 16606, 8032, 29399, 4117, 19528, 18871, 24994, 651, 293, 3066, 559, 425, 651, 9279, 12174, 12610, 8315, 36145, 29739, 4117, 2655, 13546, 25528, 15287, 293, 10458, 1832, 1975, 2655, 17082, 46152, 8315, 15844, 36145, 11296, 15287, 4724, 995, 14851, 9778, 3215, 10943, 9673, 1829, 4117, 32887, 3794, 4117, 37746, 9673, 1829, 4117, 32887, 3794, 4117, 37746, 6225, 42213, 11703, 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وظيفتها", "probability": 0.84091796875}, {"start": 375.82, "end": 376.12, "word": " أنها", "probability": 0.671142578125}, {"start": 376.12, "end": 376.8, "word": " تدخل", "probability": 0.9676513671875}, {"start": 376.8, "end": 376.92, "word": " ال", "probability": 0.91455078125}, {"start": 376.92, "end": 377.52, "word": " substrate", "probability": 0.74267578125}, {"start": 377.52, "end": 378.22, "word": " لداخل", "probability": 0.9779052734375}, {"start": 378.22, "end": 378.66, "word": " الخلية", "probability": 0.98828125}, {"start": 378.66, "end": 379.04, "word": " عشان", "probability": 0.9910481770833334}, {"start": 379.04, "end": 379.38, "word": " يصير", "probability": 0.9744466145833334}, {"start": 379.38, "end": 380.1, "word": " التفاعل", "probability": 0.9537353515625}, {"start": 380.1, "end": 381.82, "word": " Intracellular", "probability": 0.877685546875}], "temperature": 1.0}, {"id": 16, "seek": 41203, "start": 382.91, "end": 412.03, "text": "يبقى بالنسبة للـ Endoenzyme الـ enzyme يفرز داخل الخلية ال substrate ممكن تكون داخل الخلية وممكن تكون خارج الخلية إذا كانت داخل الخلية مافي عندى اي مشكلة لو كانت خارج الخلية وقتها الخلية هتفرز انزيمات هذه الانزيمات وضيفتها انها تدخل ال substrate لجوا الخلية عشان يصير التفاعل داخل الخلية من الأمثلة على الـ Endoenzyme أكسيداز كتلاز بيورياز نيترات ريدكتاز", "tokens": [1829, 3555, 4587, 7578, 20666, 1863, 35457, 3660, 24976, 39184, 6967, 78, 268, 1229, 1398, 2423, 39184, 24521, 7251, 5172, 2288, 11622, 11778, 47283, 1211, 33962, 1211, 10632, 2423, 27585, 3714, 43020, 6055, 30544, 11778, 47283, 1211, 33962, 1211, 10632, 4032, 2304, 43020, 6055, 30544, 16490, 9640, 7435, 33962, 1211, 10632, 11933, 15730, 25961, 2655, 11778, 47283, 1211, 33962, 1211, 10632, 19446, 41185, 43242, 7578, 1975, 1829, 37893, 28820, 3660, 45164, 25961, 2655, 16490, 9640, 7435, 33962, 1211, 10632, 4032, 38149, 11296, 33962, 1211, 10632, 8032, 2655, 5172, 2288, 11622, 16472, 11622, 32640, 9307, 29538, 2423, 7649, 11622, 32640, 9307, 4032, 11242, 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390.57, "word": " خارج", "probability": 0.9947916666666666}, {"start": 390.57, "end": 391.11, "word": " الخلية", "probability": 0.9921875}, {"start": 391.11, "end": 391.55, "word": " إذا", "probability": 0.89111328125}, {"start": 391.55, "end": 391.93, "word": " كانت", "probability": 0.989013671875}, {"start": 391.93, "end": 392.23, "word": " داخل", "probability": 0.9768880208333334}, {"start": 392.23, "end": 392.63, "word": " الخلية", "probability": 0.9938151041666666}, {"start": 392.63, "end": 392.93, "word": " مافي", "probability": 0.8271484375}, {"start": 392.93, "end": 393.19, "word": " عندى", "probability": 0.607666015625}, {"start": 393.19, "end": 393.31, "word": " اي", "probability": 0.598388671875}, {"start": 393.31, "end": 393.85, "word": " مشكلة", "probability": 0.99169921875}, {"start": 393.85, "end": 394.29, "word": " لو", "probability": 0.96240234375}, {"start": 394.29, "end": 394.73, "word": " كانت", "probability": 0.99462890625}, {"start": 394.73, "end": 395.05, "word": " خارج", "probability": 0.99365234375}, {"start": 395.05, "end": 395.57, "word": " الخلية", "probability": 0.99267578125}, {"start": 395.57, "end": 396.21, "word": " وقتها", "probability": 0.9920247395833334}, {"start": 396.21, "end": 396.93, "word": " الخلية", "probability": 0.9762369791666666}, {"start": 396.93, "end": 397.49, "word": " هتفرز", "probability": 0.9830078125}, {"start": 397.49, "end": 398.19, "word": " انزيمات", "probability": 0.79095458984375}, {"start": 398.19, "end": 398.35, "word": " هذه", "probability": 0.1868896484375}, {"start": 398.35, "end": 398.91, "word": " الانزيمات", "probability": 0.9220703125}, {"start": 398.91, "end": 399.51, "word": " وضيفتها", "probability": 0.874609375}, {"start": 399.51, "end": 399.77, "word": " انها", "probability": 0.7001953125}, {"start": 399.77, "end": 400.29, "word": " تدخل", "probability": 0.97412109375}, {"start": 400.29, "end": 400.41, "word": " ال", "probability": 0.90576171875}, {"start": 400.41, "end": 400.81, "word": " substrate", "probability": 0.97412109375}, {"start": 400.81, "end": 401.27, "word": " لجوا", "probability": 0.7832845052083334}, {"start": 401.27, "end": 401.65, "word": " الخلية", "probability": 0.9931640625}, {"start": 401.65, "end": 401.97, "word": " عشان", "probability": 0.9855143229166666}, {"start": 401.97, "end": 402.37, "word": " يصير", "probability": 0.9083658854166666}, {"start": 402.37, "end": 403.09, "word": " التفاعل", "probability": 0.9560546875}, {"start": 403.09, "end": 404.07, "word": " داخل", "probability": 0.986328125}, {"start": 404.07, "end": 404.65, "word": " الخلية", "probability": 0.990234375}, {"start": 404.65, "end": 405.37, "word": " من", "probability": 0.96337890625}, {"start": 405.37, "end": 406.07, "word": " الأمثلة", "probability": 0.98291015625}, {"start": 406.07, "end": 406.39, "word": " على", "probability": 0.88525390625}, {"start": 406.39, "end": 406.67, "word": " الـ", "probability": 0.756103515625}, {"start": 406.67, "end": 407.57, "word": " Endoenzyme", "probability": 0.91904296875}, {"start": 407.57, "end": 408.53, "word": " أكسيداز", "probability": 0.752392578125}, {"start": 408.53, "end": 409.65, "word": " كتلاز", "probability": 0.7813720703125}, {"start": 409.65, "end": 410.55, "word": " بيورياز", "probability": 0.8287109375}, {"start": 410.55, "end": 411.31, "word": " نيترات", "probability": 0.9195556640625}, {"start": 411.31, "end": 412.03, "word": " ريدكتاز", "probability": 0.809375}], "temperature": 1.0}, {"id": 17, "seek": 44123, "start": 412.51, "end": 441.23, "text": "طبعا هدول حفظ هنتعرف عليهم في المعامل اللي جاي هناخد كل الفحص و نتعرف على ال principle لكل الفحص و كيف بنشتغل هذا الفحص بالنسبة لـ Exoenzyme الـ Exoenzyme مكان حدوث التفاعل خارج الخلية extra cellular where substrate and enzyme react outside the cell بقول ال enzyme و ال substrate هيتفاعلوا خارج الخلية", "tokens": [9566, 3555, 3615, 995, 8032, 3215, 12610, 11331, 5172, 19913, 8032, 29399, 3615, 28480, 25894, 16095, 8978, 9673, 3615, 10943, 1211, 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0.84326171875}, {"start": 418.37, "end": 419.09, "word": " ال", "probability": 0.97412109375}, {"start": 419.09, "end": 419.59, "word": " principle", "probability": 0.496826171875}, {"start": 419.59, "end": 420.09, "word": " لكل", "probability": 0.960205078125}, {"start": 420.09, "end": 420.51, "word": " الفحص", "probability": 0.9822591145833334}, {"start": 420.51, "end": 420.67, "word": " و", "probability": 0.97998046875}, {"start": 420.67, "end": 420.89, "word": " كيف", "probability": 0.933349609375}, {"start": 420.89, "end": 421.49, "word": " بنشتغل", "probability": 0.90361328125}, {"start": 421.49, "end": 421.93, "word": " هذا", "probability": 0.9189453125}, {"start": 421.93, "end": 422.65, "word": " الفحص", "probability": 0.98291015625}, {"start": 422.65, "end": 424.05, "word": " بالنسبة", "probability": 0.927490234375}, {"start": 424.05, "end": 424.49, "word": " لـ", "probability": 0.600830078125}, {"start": 424.49, "end": 425.83, "word": " Exoenzyme", "probability": 0.8208984375}, {"start": 425.83, "end": 426.09, "word": " الـ", "probability": 0.6124267578125}, {"start": 426.09, "end": 426.95, "word": " Exoenzyme", "probability": 0.940625}, {"start": 426.95, "end": 427.41, "word": " مكان", "probability": 0.95068359375}, {"start": 427.41, "end": 427.87, "word": " حدوث", "probability": 0.9927978515625}, {"start": 427.87, "end": 428.53, "word": " التفاعل", "probability": 0.9500732421875}, {"start": 428.53, "end": 429.27, "word": " خارج", "probability": 0.9949544270833334}, {"start": 429.27, "end": 429.87, "word": " الخلية", "probability": 0.97900390625}, {"start": 429.87, "end": 430.65, "word": " extra", "probability": 0.5517578125}, {"start": 430.65, "end": 431.53, "word": " cellular", "probability": 0.5244140625}, {"start": 431.53, "end": 432.91, "word": " where", "probability": 0.86572265625}, {"start": 432.91, "end": 433.67, "word": " substrate", "probability": 0.9091796875}, {"start": 433.67, "end": 434.05, "word": " and", "probability": 0.9169921875}, {"start": 434.05, "end": 434.69, "word": " enzyme", "probability": 0.89990234375}, {"start": 434.69, "end": 435.07, "word": " react", "probability": 0.90234375}, {"start": 435.07, "end": 436.03, "word": " outside", "probability": 0.78076171875}, {"start": 436.03, "end": 436.49, "word": " the", "probability": 0.87548828125}, {"start": 436.49, "end": 436.75, "word": " cell", "probability": 0.91796875}, {"start": 436.75, "end": 437.41, "word": " بقول", "probability": 0.6505126953125}, {"start": 437.41, "end": 437.57, "word": " ال", "probability": 0.75}, {"start": 437.57, "end": 438.07, "word": " enzyme", "probability": 0.358642578125}, {"start": 438.07, "end": 438.29, "word": " و", "probability": 0.8583984375}, {"start": 438.29, "end": 438.37, "word": " ال", "probability": 0.89697265625}, {"start": 438.37, "end": 438.85, "word": " substrate", "probability": 0.9501953125}, {"start": 438.85, "end": 439.93, "word": " هيتفاعلوا", "probability": 0.89404296875}, {"start": 439.93, "end": 440.53, "word": " خارج", "probability": 0.9954427083333334}, {"start": 440.53, "end": 441.23, "word": " الخلية", "probability": 0.9864908854166666}], "temperature": 1.0}, {"id": 18, "seek": 45861, "start": 441.95, "end": 458.61, "text": "الانزايم برضه هتفرز الخلية هيفرز داخل الخلية لكن ال substrate هتكون فقط خارج الخلية احنا قلنا في ال endoenzyme ممكن تكون داخل وممكن تكون خارج لكن في ال exoenzyme ال substrate هتكون خارج الخلية", "tokens": [6027, 7649, 11622, 995, 32640, 4724, 43042, 3224, 8032, 2655, 5172, 2288, 11622, 33962, 1211, 10632, 8032, 33911, 2288, 11622, 11778, 47283, 1211, 33962, 1211, 10632, 44381, 2423, 27585, 8032, 2655, 30544, 6156, 47432, 16490, 9640, 7435, 33962, 1211, 10632, 1975, 5016, 8315, 12174, 1211, 8315, 8978, 2423, 917, 78, 268, 1229, 1398, 3714, 43020, 6055, 30544, 11778, 47283, 1211, 4032, 2304, 43020, 6055, 30544, 16490, 9640, 7435, 44381, 8978, 2423, 454, 78, 268, 1229, 1398, 2423, 27585, 8032, 2655, 30544, 16490, 9640, 7435, 33962, 1211, 10632], "avg_logprob": -0.1332563983107155, "compression_ratio": 2.0128205128205128, "no_speech_prob": 1.0728836059570312e-06, "words": [{"start": 441.95, "end": 442.75, "word": "الانزايم", "probability": 0.70634765625}, {"start": 442.75, "end": 443.33, "word": " برضه", "probability": 0.94921875}, {"start": 443.33, "end": 444.01, "word": " هتفرز", "probability": 0.915625}, {"start": 444.01, "end": 444.83, "word": " الخلية", "probability": 0.8538411458333334}, {"start": 444.83, "end": 445.47, "word": " هيفرز", "probability": 0.76678466796875}, {"start": 445.47, "end": 445.93, "word": " داخل", "probability": 0.9757486979166666}, {"start": 445.93, "end": 446.51, "word": " الخلية", "probability": 0.9853515625}, {"start": 446.51, "end": 447.39, "word": " لكن", "probability": 0.90234375}, {"start": 447.39, "end": 447.63, "word": " ال", "probability": 0.94580078125}, {"start": 447.63, "end": 448.17, "word": " substrate", "probability": 0.8232421875}, {"start": 448.17, "end": 449.15, "word": " هتكون", 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"end": 487.87, "text": "ما بقدر أدخل الـ Substrate زي ما عملت في الـ Endoenzyme لداخل الخلية لأن حجم الـ Substrate كبير عشان كده الـ Enzyme بيطلع لبرا يبقى الـ Exoenzyme الـ Enzyme بيفرز داخل الخلية بيطلع لبرا عند الـ Substrate لأن الـ Substrate حجمها كبير و بيصير التفاعل بين الـ Enzyme والـ Substrate خارج الخلية من الأمثلة على الـ Exoenzyme وحفظه", "tokens": [15042, 4724, 28543, 2288, 5551, 3215, 9778, 1211, 2423, 39184, 42090, 4404, 30767, 1829, 19446, 6225, 42213, 2655, 8978, 2423, 39184, 6967, 78, 268, 1229, 1398, 5296, 3215, 47283, 1211, 33962, 1211, 10632, 5296, 33456, 11331, 7435, 2304, 2423, 39184, 42090, 4404, 9122, 3555, 13546, 6225, 8592, 7649, 9122, 3215, 3224, 2423, 39184, 2193, 1229, 1398, 4724, 1829, 9566, 1211, 3615, 5296, 3555, 23557, 7251, 3555, 4587, 7578, 2423, 39184, 2111, 78, 268, 1229, 1398, 2423, 39184, 2193, 1229, 1398, 4724, 33911, 2288, 11622, 11778, 47283, 1211, 33962, 1211, 10632, 4724, 1829, 9566, 1211, 3615, 5296, 3555, 23557, 43242, 2423, 39184, 42090, 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" برا", "probability": 0.888916015625}, {"start": 644.33, "end": 644.79, "word": " و", "probability": 0.42236328125}, {"start": 644.79, "end": 645.13, "word": " بيصير", "probability": 0.92431640625}, {"start": 645.13, "end": 645.69, "word": " التفاعل", "probability": 0.9205322265625}, {"start": 645.69, "end": 646.11, "word": " داخل", "probability": 0.98388671875}, {"start": 646.11, "end": 646.61, "word": " الخلية،", "probability": 0.92041015625}, {"start": 646.61, "end": 646.73, "word": " لو", "probability": 0.9736328125}, {"start": 646.73, "end": 647.05, "word": " كانت", "probability": 0.9892578125}, {"start": 647.05, "end": 647.13, "word": " ال", "probability": 0.89990234375}, {"start": 647.13, "end": 647.53, "word": " substrate", "probability": 0.66357421875}, {"start": 647.53, "end": 648.03, "word": " برا", "probability": 0.90234375}, {"start": 648.03, "end": 648.35, "word": " هي", "probability": 0.53564453125}, {"start": 648.35, "end": 648.51, "word": " اللي", "probability": 0.97705078125}, {"start": 648.51, "end": 649.01, "word": " بتدخل", "probability": 0.9376220703125}, {"start": 649.01, "end": 649.71, "word": " لجوة", "probability": 0.6510009765625}, {"start": 649.71, "end": 650.53, "word": " فهذه", "probability": 0.7952473958333334}, {"start": 650.53, "end": 650.91, "word": " الجملة", "probability": 0.9453125}, {"start": 650.91, "end": 651.21, "word": " بيقول", "probability": 0.800048828125}, {"start": 651.21, "end": 651.33, "word": " لي", "probability": 0.29931640625}, {"start": 651.33, "end": 651.39, "word": " في", "probability": 0.787109375}, {"start": 651.39, "end": 651.75, "word": " عندي", "probability": 0.6754150390625}, {"start": 651.75, "end": 652.73, "word": " استثناء،", "probability": 0.827734375}, {"start": 652.73, "end": 652.89, "word": " احنا", "probability": 0.8546549479166666}, {"start": 652.89, "end": 653.33, "word": " قلنا", "probability": 0.8531901041666666}, {"start": 653.33, "end": 654.03, "word": " إنه", "probability": 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التانية الـ Endoenzyme ممكن أنه", "tokens": [2304, 43020, 2423, 39184, 6967, 78, 268, 1229, 1398, 7251, 9566, 1211, 3615, 4724, 23557, 33962, 1211, 10632, 8978, 11331, 6027, 3660, 36764, 24401, 3660, 6156, 47432, 45164, 25961, 6055, 31747, 1829, 11622, 2423, 39184, 27585, 16490, 9640, 7435, 33962, 1211, 10632, 5551, 4117, 2655, 2288, 4724, 4117, 2655, 13546, 9154, 6055, 31747, 1829, 11622, 3224, 8978, 32748, 47283, 1211, 6225, 8592, 7649, 39896, 4117, 2423, 39184, 24521, 8032, 2655, 5172, 2288, 11622, 33962, 1211, 10632, 4032, 1829, 9566, 1211, 3615, 5296, 3555, 23557, 43242, 16712, 31747, 1829, 11622, 18863, 6027, 1829, 9154, 2423, 27585, 4032, 1829, 9381, 13546, 16712, 5172, 995, 30241, 23758, 20666, 1863, 35457, 3660, 5296, 19528, 15040, 5016, 19913, 3660, 16712, 7649, 10632, 2423, 39184, 6967, 78, 268, 1229, 1398, 3714, 43020, 14739, 3224], "avg_logprob": -0.07854893410852713, "compression_ratio": 1.9227467811158798, "no_speech_prob": 3.635883331298828e-06, "words": [{"start": 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"word": " تركيز", "probability": 0.9947509765625}, {"start": 667.5, "end": 667.76, "word": " الـ", "probability": 0.87939453125}, {"start": 667.76, "end": 668.34, "word": " substrate", "probability": 0.4716796875}, {"start": 668.34, "end": 669.04, "word": " خارج", "probability": 0.9910481770833334}, {"start": 669.04, "end": 669.72, "word": " الخلية", "probability": 0.9894205729166666}, {"start": 669.72, "end": 670.76, "word": " أكتر", "probability": 0.9793701171875}, {"start": 670.76, "end": 671.32, "word": " بكتير", "probability": 0.9837646484375}, {"start": 671.32, "end": 671.48, "word": " من", "probability": 0.9951171875}, {"start": 671.48, "end": 672.16, "word": " تركيزه", "probability": 0.9962890625}, {"start": 672.16, "end": 672.26, "word": " في", "probability": 0.95849609375}, {"start": 672.26, "end": 672.82, "word": " الداخل", "probability": 0.9900716145833334}, {"start": 672.82, "end": 673.7, "word": " عشان", "probability": 0.947265625}, {"start": 673.7, "end": 674.24, "word": " هيك", "probability": 0.66650390625}, {"start": 674.24, "end": 674.72, "word": " الـ", "probability": 0.9130859375}, {"start": 674.72, "end": 675.08, "word": " enzyme", "probability": 0.888671875}, {"start": 675.08, "end": 675.7, "word": " هتفرز", "probability": 0.93203125}, {"start": 675.7, "end": 676.22, "word": " الخلية", "probability": 0.9772135416666666}, {"start": 676.22, "end": 676.8, "word": " ويطلع", "probability": 0.88544921875}, {"start": 676.8, "end": 677.24, "word": " لبرا", "probability": 0.8642578125}, {"start": 677.24, "end": 677.52, "word": " عند", "probability": 0.9658203125}, {"start": 677.52, "end": 678.12, "word": " التركيز", "probability": 0.993896484375}, {"start": 678.12, "end": 678.58, "word": " العالي", "probability": 0.986328125}, {"start": 678.58, "end": 678.74, "word": " من", "probability": 0.990234375}, {"start": 678.74, "end": 678.88, "word": " ال", "probability": 0.9794921875}, {"start": 678.88, "end": 679.44, "word": " substrate", "probability": 0.495361328125}, {"start": 679.44, "end": 680.46, "word": " ويصير", "probability": 0.951171875}, {"start": 680.46, "end": 681.3, "word": " التفاعل", "probability": 0.919677734375}, {"start": 681.3, "end": 682.26, "word": " هذا", "probability": 0.8564453125}, {"start": 682.26, "end": 682.9, "word": " بالنسبة", "probability": 0.9937744140625}, {"start": 682.9, "end": 683.98, "word": " للملاحظة", "probability": 0.9345703125}, {"start": 683.98, "end": 684.48, "word": " التانية", "probability": 0.9718424479166666}, {"start": 684.48, "end": 684.68, "word": " الـ", "probability": 0.955078125}, {"start": 684.68, "end": 685.36, "word": " Endoenzyme", "probability": 0.92431640625}, {"start": 685.36, "end": 685.86, "word": " ممكن", "probability": 0.9873046875}, {"start": 685.86, "end": 686.42, "word": " أنه", "probability": 0.796630859375}], "temperature": 1.0}, {"id": 27, "seek": 71390, "start": 687.64, "end": 713.9, "text": "يطلع خارج الخلية في حال كان عندي الـ Concentration of its substrate تركيزه خارج الخلية أعلى من داخل الخلية آخر ملاحظة The same enzyme could be inducible and constitute in different genera كاتب مثال اليورياسي أحنا قلنا أن الإنزايم يقسم حسب الإنتاج إلى inducible and constitutive", "tokens": [1829, 9566, 1211, 3615, 16490, 9640, 7435, 33962, 1211, 10632, 8978, 11331, 6027, 25961, 18871, 16254, 2423, 39184, 2656, 2207, 2405, 295, 1080, 27585, 6055, 31747, 1829, 11622, 3224, 16490, 9640, 7435, 33962, 1211, 10632, 5551, 3615, 23942, 9154, 11778, 47283, 1211, 33962, 1211, 10632, 19753, 34740, 3714, 15040, 5016, 19913, 3660, 440, 912, 24521, 727, 312, 13716, 32128, 293, 23079, 1169, 294, 819, 1337, 64, 9122, 9307, 3555, 50113, 6027, 45595, 13063, 25528, 3794, 1829, 5551, 5016, 8315, 12174, 1211, 8315, 14739, 33688, 1863, 11622, 995, 32640, 7251, 4587, 38251, 11331, 35457, 33688, 29399, 26108, 30731, 13716, 32128, 293, 23079, 17254], "avg_logprob": -0.21192354166391983, "compression_ratio": 1.5708661417322836, "no_speech_prob": 2.8014183044433594e-06, "words": 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693.12, "end": 694.08, "word": " تركيزه", "probability": 0.94990234375}, {"start": 694.08, "end": 694.42, "word": " خارج", "probability": 0.9899088541666666}, {"start": 694.42, "end": 694.88, "word": " الخلية", "probability": 0.9881184895833334}, {"start": 694.88, "end": 695.24, "word": " أعلى", "probability": 0.9318033854166666}, {"start": 695.24, "end": 695.42, "word": " من", "probability": 0.99462890625}, {"start": 695.42, "end": 695.9, "word": " داخل", "probability": 0.9666341145833334}, {"start": 695.9, "end": 696.5, "word": " الخلية", "probability": 0.9869791666666666}, {"start": 696.5, "end": 697.6, "word": " آخر", "probability": 0.736083984375}, {"start": 697.6, "end": 698.44, "word": " ملاحظة", "probability": 0.9908203125}, {"start": 698.44, "end": 698.88, "word": " The", "probability": 0.487060546875}, {"start": 698.88, "end": 699.26, "word": " same", "probability": 0.88818359375}, {"start": 699.26, "end": 699.9, "word": " enzyme", "probability": 0.9482421875}, {"start": 699.9, "end": 700.26, "word": " could", "probability": 0.9375}, {"start": 700.26, "end": 700.64, "word": " be", "probability": 0.95751953125}, {"start": 700.64, "end": 701.46, "word": " inducible", "probability": 0.825927734375}, {"start": 701.46, "end": 701.98, "word": " and", "probability": 0.927734375}, {"start": 701.98, "end": 702.84, "word": " constitute", "probability": 0.622802734375}, {"start": 702.84, "end": 703.1, "word": " in", "probability": 0.351318359375}, {"start": 703.1, "end": 703.58, "word": " different", "probability": 0.89208984375}, {"start": 703.58, "end": 704.78, "word": " genera", "probability": 0.57373046875}, {"start": 704.78, "end": 705.16, "word": " كاتب", "probability": 0.6923828125}, {"start": 705.16, "end": 705.64, "word": " مثال", "probability": 0.8369140625}, {"start": 705.64, "end": 707.4, "word": " اليورياسي", "probability": 0.65224609375}, {"start": 707.4, "end": 707.96, "word": " أحنا", "probability": 0.7179768880208334}, {"start": 707.96, "end": 708.32, "word": " قلنا", "probability": 0.9163411458333334}, {"start": 708.32, "end": 708.54, "word": " أن", "probability": 0.46826171875}, {"start": 708.54, "end": 709.22, "word": " الإنزايم", "probability": 0.690185546875}, {"start": 709.22, "end": 709.7, "word": " يقسم", "probability": 0.9117838541666666}, {"start": 709.7, "end": 710.22, "word": " حسب", "probability": 0.97802734375}, {"start": 710.22, "end": 711.02, "word": " الإنتاج", "probability": 0.9300130208333334}, {"start": 711.02, "end": 711.36, "word": " إلى", "probability": 0.96728515625}, {"start": 711.36, "end": 712.2, "word": " inducible", "probability": 0.7012939453125}, {"start": 712.2, "end": 712.8, "word": " and", "probability": 0.94873046875}, {"start": 712.8, "end": 713.9, "word": " constitutive", "probability": 0.7744140625}], "temperature": 1.0}, {"id": 28, "seek": 73858, "start": 714.6, "end": 738.58, "text": "هذه الجملة تقول أن الانزيم نفسه مثلا X ممكن يكون inducible في بكتيريا معينة وconstitute في بكتيريا أخرى من الأمثلة على هذا الانزيم اللي ممكن يكون inducible في بكتيريا وconstitute في بكتيريا أخرى اليوريازي اليورياز من اسمه يورياز هيكسر اليوريا", "tokens": [3224, 24192, 25724, 2304, 37977, 6055, 39648, 14739, 2423, 7649, 11622, 32640, 8717, 36178, 3224, 50113, 15040, 1783, 3714, 43020, 7251, 30544, 13716, 32128, 8978, 4724, 4117, 2655, 13546, 25528, 20449, 9957, 3660, 4032, 25279, 6559, 8978, 4724, 4117, 2655, 13546, 25528, 5551, 34740, 7578, 9154, 16247, 2304, 12984, 37977, 15844, 23758, 2423, 7649, 11622, 32640, 13672, 1829, 3714, 43020, 7251, 30544, 13716, 32128, 8978, 4724, 4117, 2655, 13546, 25528, 4032, 25279, 6559, 8978, 4724, 4117, 2655, 13546, 25528, 5551, 34740, 7578, 45595, 13063, 1829, 31377, 1829, 45595, 13063, 1829, 31377, 9154, 24525, 2304, 3224, 7251, 13063, 1829, 31377, 39896, 4117, 3794, 2288, 45595, 13063, 25528], "avg_logprob": -0.1525993049144745, "compression_ratio": 2.2185792349726774, "no_speech_prob": 2.384185791015625e-07, "words": [{"start": 714.6, "end": 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" negative", "probability": 0.87255859375}, {"start": 2114.36, "end": 2128.86, "word": " لو", "probability": 0.796875}, {"start": 2128.86, "end": 2129.46, "word": " كانت", "probability": 0.989013671875}, {"start": 2129.46, "end": 2129.96, "word": " negative", "probability": 0.92333984375}, {"start": 2129.96, "end": 2130.38, "word": " يبقى", "probability": 0.9586181640625}, {"start": 2130.38, "end": 2130.72, "word": " هي", "probability": 0.9609375}, {"start": 2130.72, "end": 2130.94, "word": " a", "probability": 0.195556640625}, {"start": 2130.94, "end": 2131.2, "word": " closed", "probability": 0.92822265625}, {"start": 2131.2, "end": 2131.62, "word": " medium", "probability": 0.50439453125}], "temperature": 1.0}, {"id": 73, "seek": 219560, "start": 2166.78, "end": 2195.6, "text": "لكن لو كانت positive فى عندى احتمالين اما انها تكون bustle loss او انها تكون listeria monocytogen bustle loss or listeria mono", "tokens": [1211, 19452, 45164, 25961, 2655, 3353, 6156, 7578, 43242, 7578, 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فى اندي air bubbles يبقى كتلاس بوزيتف لكن هن فى اندي smooth suspension يبقى هن نيجاتف للكتلس هيك اللى هى ال result", "tokens": [1863, 36178, 27188, 4117, 25720, 24976, 3794, 15040, 1829, 3215, 3714, 1829, 12984, 23328, 8717, 36178, 27188, 4117, 25720, 6156, 7578, 16472, 16254, 1988, 16295, 7251, 3555, 4587, 7578, 9122, 2655, 1211, 32277, 4724, 2407, 11622, 36081, 5172, 44381, 8032, 1863, 6156, 7578, 16472, 16254, 5508, 15771, 7251, 3555, 4587, 7578, 8032, 1863, 8717, 1829, 7435, 9307, 5172, 5296, 23275, 2655, 1211, 3794, 39896, 4117, 13672, 7578, 8032, 7578, 2423, 1874], "avg_logprob": -0.4687499958607886, "compression_ratio": 1.5751633986928104, "no_speech_prob": 7.033348083496094e-06, "words": [{"start": 2625.8799999999997, "end": 2626.64, "word": "نفس", "probability": 0.333251953125}, {"start": 2626.64, "end": 2626.64, "word": " الفكرة", "probability": 0.8333333333333334}, {"start": 2626.64, "end": 2627.96, "word": " للسلايد", "probability": 0.454620361328125}, {"start": 2627.96, 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طيب ليش أنا ممنوع أخد كولوني من البلد أجار", "tokens": [4587, 1211, 8315, 29739, 1211, 3215, 5551, 7435, 9640, 39896, 2193, 10794, 292, 14741, 9154, 11242, 33911, 29739, 1211, 3215, 13672, 7578, 31439, 15436, 7251, 34268, 26890, 44945, 35457, 3660, 1025, 4, 37495, 22653, 45164, 41850, 11331, 11242, 43500, 502, 2733, 9154, 2423, 32694, 5551, 7435, 9640, 4724, 11242, 33911, 2625, 23271, 9154, 29739, 1211, 3215, 23032, 3555, 3615, 995, 39182, 19446, 5551, 3615, 995, 4587, 2304, 2423, 3021, 4032, 6055, 9566, 1211, 3615, 9154, 2423, 45833, 27995, 4724, 14851, 1863, 11296, 6055, 26890, 3215, 39182, 9957, 4724, 11242, 33911, 29739, 1211, 3215, 6225, 8592, 7649, 19446, 7251, 2655, 4117, 3794, 2288, 23032, 1829, 3555, 32239, 8592, 41850, 3714, 27842, 45367, 5551, 9778, 3215, 9122, 12610, 11536, 1829, 9154, 29739, 1211, 3215, 5551, 7435, 9640], "avg_logprob": -0.19494047950184534, "compression_ratio": 1.6996197718631179, "no_speech_prob": 8.463859558105469e-06, "words": [{"start": 2684.0, "end": 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false positive result ليش؟ لأنك لما تاخدي كولوني من إبلاد أجار ممكن تروح تاخدي مع الـ colony تجرح الأجار يعني تاخدي شوية media يعني blood قولنا لـ blood cells نفسها بتحتوي على Catalase enzyme بالتالي", "tokens": [3555, 39648, 11703, 1829, 45164, 9122, 29399, 30767, 9640, 27884, 4724, 4117, 2655, 13546, 25528, 15844, 11933, 36150, 18513, 5551, 7435, 9640, 4032, 25957, 1211, 31371, 6156, 5016, 9381, 2423, 39184, 24994, 651, 3714, 43020, 37495, 9566, 9957, 1829, 7908, 3353, 1874, 32239, 8592, 22807, 5296, 33456, 4117, 5296, 15042, 6055, 47283, 16254, 9122, 12610, 11536, 1829, 9154, 11933, 36150, 18513, 5551, 7435, 9640, 3714, 43020, 6055, 32887, 5016, 6055, 47283, 16254, 20449, 2423, 39184, 23028, 6055, 7435, 2288, 5016, 16247, 7435, 9640, 37495, 22653, 6055, 47283, 16254, 13412, 2407, 10632, 3021, 37495, 22653, 3390, 12174, 12610, 8315, 5296, 39184, 3390, 5438, 8717, 36178, 11296, 39894, 33753, 45865, 15844, 24994, 651, 24521, 20666, 2655, 6027, 1829], "avg_logprob": 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{"start": 2724.49, "end": 2724.89, "word": " تاخدي", "probability": 0.9095052083333334}, {"start": 2724.89, "end": 2725.35, "word": " شوية", "probability": 0.9352213541666666}, {"start": 2725.35, "end": 2725.89, "word": " media", "probability": 0.65576171875}, {"start": 2725.89, "end": 2726.85, "word": " يعني", "probability": 0.937744140625}, {"start": 2726.85, "end": 2727.45, "word": " blood", "probability": 0.578125}, {"start": 2727.45, "end": 2728.19, "word": " قولنا", "probability": 0.7936197916666666}, {"start": 2728.19, "end": 2728.37, "word": " لـ", "probability": 0.771240234375}, {"start": 2728.37, "end": 2728.71, "word": " blood", "probability": 0.476806640625}, {"start": 2728.71, "end": 2729.35, "word": " cells", "probability": 0.88330078125}, {"start": 2729.35, "end": 2729.99, "word": " نفسها", "probability": 0.9773763020833334}, {"start": 2729.99, "end": 2730.69, "word": " بتحتوي", "probability": 0.8279622395833334}, {"start": 2730.69, "end": 2731.05, "word": " على", "probability": 0.9462890625}, {"start": 2731.05, "end": 2731.77, "word": " Catalase", "probability": 0.8466796875}, {"start": 2731.77, "end": 2732.37, "word": " enzyme", "probability": 0.92919921875}, {"start": 2732.37, "end": 2733.83, "word": " بالتالي", "probability": 0.901611328125}], "temperature": 1.0}, {"id": 96, "seek": 275399, "start": 2734.17, "end": 2753.99, "text": "ممكن البكتيريا ما يكون عندها أنزيم الـ Catalase لكن انت أخدت كله عن طريق أو من طبق ال blood agar و جرحت هذا ال agar يعني أخدت blood اللي موجود فيه Catalase enzyme بالتالي هياتفاعل مع ال substrate اللي احنا حاطينها ال H2O2 وبعطيني positive result", "tokens": [2304, 43020, 29739, 4117, 2655, 13546, 25528, 19446, 7251, 30544, 43242, 11296, 14739, 11622, 32640, 2423, 39184, 24994, 651, 44381, 16472, 2655, 5551, 9778, 3215, 2655, 28242, 3224, 18871, 23032, 16572, 4587, 34051, 9154, 23032, 3555, 4587, 2423, 3390, 623, 289, 4032, 10874, 2288, 33753, 23758, 2423, 623, 289, 37495, 22653, 5551, 9778, 3215, 2655, 3390, 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"end": 2741.61, "word": " agar", "probability": 0.847900390625}, {"start": 2741.61, "end": 2741.75, "word": " و", "probability": 0.5400390625}, {"start": 2741.75, "end": 2742.11, "word": " جرحت", "probability": 0.845703125}, {"start": 2742.11, "end": 2742.39, "word": " هذا", "probability": 0.95556640625}, {"start": 2742.39, "end": 2742.53, "word": " ال", "probability": 0.9189453125}, {"start": 2742.53, "end": 2742.93, "word": " agar", "probability": 0.6522216796875}, {"start": 2742.93, "end": 2743.47, "word": " يعني", "probability": 0.911376953125}, {"start": 2743.47, "end": 2743.93, "word": " أخدت", "probability": 0.9765625}, {"start": 2743.93, "end": 2744.33, "word": " blood", "probability": 0.88134765625}, {"start": 2744.33, "end": 2744.59, "word": " اللي", "probability": 0.876708984375}, {"start": 2744.59, "end": 2745.09, "word": " موجود", "probability": 0.97705078125}, {"start": 2745.09, "end": 2745.37, "word": " فيه", "probability": 0.820068359375}, {"start": 2745.37, "end": 2746.01, "word": " Catalase", "probability": 0.705810546875}, {"start": 2746.01, "end": 2746.53, "word": " enzyme", "probability": 0.70703125}, {"start": 2746.53, "end": 2747.05, "word": " بالتالي", "probability": 0.8460693359375}, {"start": 2747.05, "end": 2747.79, "word": " هياتفاعل", "probability": 0.85}, {"start": 2747.79, "end": 2747.99, "word": " مع", "probability": 0.98388671875}, {"start": 2747.99, "end": 2748.11, "word": " ال", "probability": 0.93017578125}, {"start": 2748.11, "end": 2748.49, "word": " substrate", "probability": 0.416015625}, {"start": 2748.49, "end": 2748.75, "word": " اللي", "probability": 0.9716796875}, {"start": 2748.75, "end": 2748.97, "word": " احنا", "probability": 0.8776041666666666}, {"start": 2748.97, "end": 2749.75, "word": " حاطينها", "probability": 0.8642578125}, {"start": 2749.75, "end": 2750.33, "word": " ال", "probability": 0.65869140625}, {"start": 2750.33, "end": 2751.91, "word": " H2O2", "probability": 0.81829833984375}, {"start": 2751.91, "end": 2752.87, "word": " وبعطيني", "probability": 0.743896484375}, {"start": 2752.87, "end": 2753.27, "word": " positive", "probability": 0.763671875}, {"start": 2753.27, "end": 2753.99, "word": " result", "probability": 0.958984375}], "temperature": 1.0}, {"id": 97, "seek": 277661, "start": 2754.71, "end": 2776.61, "text": "عشان هيك او بيعطيني false positive result نتيجة خاطئة مش البكتيريا عندها ال enzyme عشان هيك الأفضل اني انا اشتغل اي فحص كيميائي على general media على nutrient agar لو اضطريت اني انا ااخد من blood agar بحاول اني انا ااخد بدون ما اجرح ال media avoid touching the agar by the loop", "tokens": [3615, 8592, 7649, 39896, 4117, 1975, 2407, 4724, 1829, 3615, 9566, 1829, 22653, 7908, 3353, 1874, 8717, 31371, 7435, 3660, 16490, 41193, 19986, 3660, 37893, 29739, 4117, 2655, 13546, 25528, 43242, 11296, 2423, 24521, 6225, 8592, 7649, 39896, 4117, 16247, 5172, 11242, 1211, 16472, 1829, 1975, 8315, 1975, 8592, 2655, 17082, 1211, 1975, 1829, 6156, 5016, 9381, 9122, 32640, 1829, 16373, 1829, 15844, 2674, 3021, 15844, 32694, 623, 289, 45164, 1975, 11242, 9566, 16572, 2655, 16472, 1829, 1975, 8315, 1975, 47283, 3215, 9154, 3390, 623, 289, 4724, 5016, 995, 12610, 16472, 1829, 1975, 8315, 1975, 47283, 3215, 47525, 11536, 19446, 1975, 7435, 2288, 5016, 2423, 3021, 5042, 11175, 264, 623, 289, 538, 264, 6367], "avg_logprob": -0.14279891485753266, "compression_ratio": 1.6334661354581674, "no_speech_prob": 4.76837158203125e-07, "words": [{"start": 2754.71, "end": 2755.21, "word": "عشان", "probability": 0.85400390625}, {"start": 2755.21, "end": 2755.59, "word": " هيك", "probability": 0.7265625}, {"start": 2755.59, "end": 2755.79, "word": " او", "probability": 0.6505126953125}, {"start": 2755.79, "end": 2756.41, "word": " بيعطيني", "probability": 0.763671875}, {"start": 2756.41, "end": 2756.71, "word": " false", "probability": 0.5048828125}, {"start": 2756.71, "end": 2757.23, "word": " positive", "probability": 0.93017578125}, {"start": 2757.23, "end": 2757.63, "word": " 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"word": " انا", "probability": 0.8232421875}, {"start": 2762.53, "end": 2763.05, "word": " اشتغل", "probability": 0.9412109375}, {"start": 2763.05, "end": 2763.31, "word": " اي", "probability": 0.90869140625}, {"start": 2763.31, "end": 2763.67, "word": " فحص", "probability": 0.9220377604166666}, {"start": 2763.67, "end": 2764.21, "word": " كيميائي", "probability": 0.95810546875}, {"start": 2764.21, "end": 2764.47, "word": " على", "probability": 0.916015625}, {"start": 2764.47, "end": 2765.03, "word": " general", "probability": 0.822265625}, {"start": 2765.03, "end": 2765.47, "word": " media", "probability": 0.9296875}, {"start": 2765.47, "end": 2765.81, "word": " على", "probability": 0.802734375}, {"start": 2765.81, "end": 2766.43, "word": " nutrient", "probability": 0.873046875}, {"start": 2766.43, "end": 2766.89, "word": " agar", "probability": 0.9482421875}, {"start": 2766.89, "end": 2767.55, "word": " لو", "probability": 0.94482421875}, {"start": 2767.55, "end": 2768.17, "word": " اضطريت", "probability": 0.90830078125}, {"start": 2768.17, "end": 2768.45, "word": " اني", "probability": 0.90625}, {"start": 2768.45, "end": 2768.63, "word": " انا", "probability": 0.932373046875}, {"start": 2768.63, "end": 2769.13, "word": " ااخد", "probability": 0.7985026041666666}, {"start": 2769.13, "end": 2769.31, "word": " من", "probability": 0.9970703125}, {"start": 2769.31, "end": 2769.65, "word": " blood", "probability": 0.83642578125}, {"start": 2769.65, "end": 2770.11, "word": " agar", "probability": 0.975341796875}, {"start": 2770.11, "end": 2771.41, "word": " بحاول", "probability": 0.9813232421875}, {"start": 2771.41, "end": 2771.71, "word": " اني", "probability": 0.715576171875}, {"start": 2771.71, "end": 2771.83, "word": " انا", "probability": 0.900634765625}, {"start": 2771.83, "end": 2772.17, "word": " ااخد", "probability": 0.8935546875}, {"start": 2772.17, "end": 2772.57, "word": " بدون", "probability": 0.9921875}, {"start": 2772.57, "end": 2772.73, "word": " ما", "probability": 0.7099609375}, {"start": 2772.73, "end": 2773.31, "word": " اجرح", "probability": 0.9676513671875}, {"start": 2773.31, "end": 2773.85, "word": " ال", "probability": 0.958984375}, {"start": 2773.85, "end": 2774.15, "word": " media", "probability": 0.97705078125}, {"start": 2774.15, "end": 2774.77, "word": " avoid", "probability": 0.6865234375}, {"start": 2774.77, "end": 2775.41, "word": " touching", "probability": 0.87841796875}, {"start": 2775.41, "end": 2775.67, "word": " the", "probability": 0.8955078125}, {"start": 2775.67, "end": 2776.01, "word": " agar", "probability": 0.96826171875}, {"start": 2776.01, "end": 2776.25, "word": " by", "probability": 0.951171875}, {"start": 2776.25, "end": 2776.43, "word": " the", "probability": 0.90234375}, {"start": 2776.43, "end": 2776.61, "word": " loop", "probability": 0.73779296875}], "temperature": 1.0}, {"id": 98, "seek": 280314, "start": 2777.42, "end": 2803.14, "text": "هذا بالنسبة للملاحظة الأولى الملاحظة التانية also don't use bacteria from old culture because the enzyme activity drop by time بيعطيني false negative اي فحص كيميائي لازم اني انا اشتغله تكون ال culture fresh لأنه لو كانت old culture بتكون البكتيريا منهجة تمام ال enzyme activity بيبدأ EL فبالتالي بيعطيني false negative result", "tokens": [3224, 15730, 20666, 1863, 35457, 3660, 5296, 19528, 15040, 5016, 19913, 3660, 16247, 12610, 7578, 9673, 15040, 5016, 19913, 3660, 16712, 7649, 10632, 611, 500, 380, 764, 11763, 490, 1331, 3713, 570, 264, 24521, 5191, 3270, 538, 565, 4724, 40228, 9566, 1829, 22653, 7908, 3671, 1975, 1829, 6156, 5016, 9381, 9122, 32640, 1829, 16373, 1829, 5296, 31377, 2304, 16472, 1829, 1975, 8315, 1975, 8592, 2655, 17082, 1211, 3224, 6055, 30544, 2423, 3713, 4451, 5296, 33456, 3224, 45164, 25961, 2655, 1331, 3713, 39894, 30544, 29739, 4117, 2655, 13546, 25528, 9154, 3224, 7435, 3660, 46811, 10943, 2423, 24521, 5191, 4724, 1829, 44510, 10721, 14426, 6156, 3555, 6027, 2655, 6027, 1829, 4724, 40228, 9566, 1829, 22653, 7908, 3671, 1874], "avg_logprob": -0.15251068477956659, "compression_ratio": 1.6911764705882353, "no_speech_prob": 4.76837158203125e-07, "words": [{"start": 2777.42, "end": 2777.78, "word": "هذا", "probability": 0.874267578125}, {"start": 2777.78, "end": 2778.34, "word": " بالنسبة", "probability": 0.9754638671875}, {"start": 2778.34, "end": 2779.52, "word": " للملاحظة", "probability": 0.9046223958333334}, {"start": 2779.52, "end": 2779.9, "word": " الأولى", "probability": 0.97119140625}, {"start": 2779.9, "end": 2780.52, "word": " الملاحظة", "probability": 0.9607421875}, {"start": 2780.52, "end": 2780.92, "word": " التانية", "probability": 0.9563802083333334}, {"start": 2780.92, "end": 2781.34, "word": " also", "probability": 0.06549072265625}, {"start": 2781.34, "end": 2781.66, "word": " don't", "probability": 0.87841796875}, {"start": 2781.66, "end": 2781.96, "word": " use", "probability": 0.86474609375}, {"start": 2781.96, "end": 2782.46, "word": " bacteria", "probability": 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0.74462890625}, {"start": 2787.7, "end": 2788.14, "word": " negative", "probability": 0.9169921875}, {"start": 2788.14, "end": 2789.1, "word": " اي", "probability": 0.6221923828125}, {"start": 2789.1, "end": 2789.46, "word": " فحص", "probability": 0.9723307291666666}, {"start": 2789.46, "end": 2790.02, "word": " كيميائي", "probability": 0.946875}, {"start": 2790.02, "end": 2790.32, "word": " لازم", "probability": 0.97119140625}, {"start": 2790.32, "end": 2790.52, "word": " اني", "probability": 0.5650634765625}, {"start": 2790.52, "end": 2790.66, "word": " انا", "probability": 0.841796875}, {"start": 2790.66, "end": 2791.16, "word": " اشتغله", "probability": 0.9033203125}, {"start": 2791.16, "end": 2791.48, "word": " تكون", "probability": 0.971923828125}, {"start": 2791.48, "end": 2791.64, "word": " ال", "probability": 0.94921875}, {"start": 2791.64, "end": 2792.1, "word": " culture", "probability": 0.94384765625}, {"start": 2792.1, "end": 2792.82, "word": " fresh", "probability": 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"probability": 0.94775390625}, {"start": 2799.06, "end": 2799.62, "word": " بيبدأ", "probability": 0.971435546875}, {"start": 2799.62, "end": 2800.04, "word": " EL", "probability": 0.378662109375}, {"start": 2800.04, "end": 2800.78, "word": " فبالتالي", "probability": 0.9883626302083334}, {"start": 2800.78, "end": 2801.38, "word": " بيعطيني", "probability": 0.83388671875}, {"start": 2801.38, "end": 2801.94, "word": " false", "probability": 0.8583984375}, {"start": 2801.94, "end": 2802.54, "word": " negative", "probability": 0.9599609375}, {"start": 2802.54, "end": 2803.14, "word": " result", "probability": 0.9150390625}], "temperature": 1.0}, {"id": 99, "seek": 283257, "start": 2804.25, "end": 2832.57, "text": "آخر ملاحظة احنا هنضيفها و احنا نحكيها قبل ما نختم بالـ Catalase Test هلأ مش كل البكتيريا قلنا عندها Enzyme الـ Catalase على سبيل المثال قلنا الـ Streptococci ما عندها Enzyme الـ Catalase طب قلنا خلال التنفس الخلوي بيتم إنتاج الـ H2O2 اللي هو يعتبر toxic worst product كيف البكتيريا اللي ما عندها Enzyme الـ Catalase هتتخلص من الـ H2O2 اللي يعتبر toxic", "tokens": [148, 95, 34740, 3714, 15040, 5016, 19913, 3660, 1975, 5016, 8315, 8032, 1863, 11242, 33911, 11296, 4032, 1975, 5016, 8315, 8717, 5016, 4117, 1829, 11296, 12174, 36150, 19446, 8717, 9778, 39237, 20666, 39184, 24994, 651, 9279, 8032, 1211, 10721, 37893, 28242, 29739, 4117, 2655, 13546, 25528, 12174, 1211, 8315, 43242, 11296, 2193, 1229, 1398, 2423, 39184, 24994, 651, 15844, 8608, 21292, 1211, 9673, 12984, 6027, 12174, 1211, 8315, 2423, 39184, 19597, 662, 905, 905, 537, 19446, 43242, 11296, 2193, 1229, 1398, 2423, 39184, 24994, 651, 23032, 3555, 12174, 1211, 8315, 16490, 1211, 6027, 16712, 1863, 36178, 33962, 1211, 45865, 4724, 36081, 2304, 11933, 29399, 26108, 2423, 39184, 389, 17, 46, 17, 13672, 1829, 31439, 7251, 34268, 26890, 12786, 5855, 1674, 9122, 33911, 29739, 4117, 2655, 13546, 25528, 13672, 1829, 19446, 43242, 11296, 2193, 1229, 1398, 2423, 39184, 24994, 651, 8032, 2655, 2655, 9778, 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"probability": 0.981689453125}, {"start": 2817.91, "end": 2818.47, "word": " طب", "probability": 0.909423828125}, {"start": 2818.47, "end": 2818.77, "word": " قلنا", "probability": 0.97900390625}, {"start": 2818.77, "end": 2819.15, "word": " خلال", "probability": 0.9949544270833334}, {"start": 2819.15, "end": 2819.59, "word": " التنفس", "probability": 0.9650065104166666}, {"start": 2819.59, "end": 2820.11, "word": " الخلوي", "probability": 0.9620768229166666}, {"start": 2820.11, "end": 2820.55, "word": " بيتم", "probability": 0.8904622395833334}, {"start": 2820.55, "end": 2821.03, "word": " إنتاج", "probability": 0.9013671875}, {"start": 2821.03, "end": 2821.27, "word": " الـ", "probability": 0.8876953125}, {"start": 2821.27, "end": 2822.27, "word": " H2O2", "probability": 0.870849609375}, {"start": 2822.27, "end": 2822.79, "word": " اللي", "probability": 0.875}, {"start": 2822.79, "end": 2822.91, "word": " هو", "probability": 0.98291015625}, {"start": 2822.91, "end": 2823.39, "word": " يعتبر", "probability": 0.8943684895833334}, {"start": 2823.39, "end": 2824.01, "word": " toxic", "probability": 0.4267578125}, {"start": 2824.01, "end": 2825.31, "word": " worst", "probability": 0.48974609375}, {"start": 2825.31, "end": 2825.93, "word": " product", "probability": 0.947265625}, {"start": 2825.93, "end": 2826.57, "word": " كيف", "probability": 0.9755859375}, {"start": 2826.57, "end": 2827.27, "word": " البكتيريا", "probability": 0.973828125}, {"start": 2827.27, "end": 2827.53, "word": " اللي", "probability": 0.944091796875}, {"start": 2827.53, "end": 2827.69, "word": " ما", "probability": 0.98876953125}, {"start": 2827.69, "end": 2828.07, "word": " عندها", "probability": 0.980224609375}, {"start": 2828.07, "end": 2828.45, "word": " Enzyme", "probability": 0.91259765625}, {"start": 2828.45, "end": 2828.67, "word": " الـ", "probability": 0.682373046875}, {"start": 2828.67, "end": 2829.15, "word": " Catalase", "probability": 0.98095703125}, {"start": 2829.15, "end": 2829.95, "word": " هتتخلص", "probability": 0.9864095052083334}, {"start": 2829.95, "end": 2830.15, "word": " من", "probability": 0.994140625}, {"start": 2830.15, "end": 2830.39, "word": " الـ", "probability": 0.980712890625}, {"start": 2830.39, "end": 2831.33, "word": " H2O2", "probability": 0.930908203125}, {"start": 2831.33, "end": 2831.57, "word": " اللي", "probability": 0.97021484375}, {"start": 2831.57, "end": 2832.11, "word": " يعتبر", "probability": 0.9508463541666666}, {"start": 2832.11, "end": 2832.57, "word": " toxic", "probability": 0.841796875}], "temperature": 1.0}, {"id": 100, "seek": 285688, "start": 2832.8, "end": 2856.88, "text": "وقلنا ان البكتيريا اللى معاها عندها انزيم الـ catalase عندها انزيم آخر بيقوم بنفس الوظيفة اللى هو اسمه hydrogen peroxidase من اسمه hydrogen peroxidase يعني بيكسر ال hydrogen peroxide هيشتغل على نصف ال substrate اللى هى ال H2O2 لكن هيعطينى جزيئان ميه هيعطينى two جزيئان H2O2", "tokens": [30543, 1211, 8315, 16472, 29739, 4117, 2655, 13546, 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{"start": 2833.56, "end": 2834.1, "word": " البكتيريا", "probability": 0.954296875}, {"start": 2834.1, "end": 2834.24, "word": " اللى", "probability": 0.8134765625}, {"start": 2834.24, "end": 2834.44, "word": " معاها", "probability": 0.849609375}, {"start": 2834.44, "end": 2834.68, "word": " عندها", "probability": 0.95751953125}, {"start": 2834.68, "end": 2835.06, "word": " انزيم", "probability": 0.8074544270833334}, {"start": 2835.06, "end": 2835.3, "word": " الـ", "probability": 0.59033203125}, {"start": 2835.3, "end": 2835.78, "word": " catalase", "probability": 0.631103515625}, {"start": 2835.78, "end": 2836.16, "word": " عندها", "probability": 0.971923828125}, {"start": 2836.16, "end": 2836.72, "word": " انزيم", "probability": 0.9485677083333334}, {"start": 2836.72, "end": 2837.36, "word": " آخر", "probability": 0.8779296875}, {"start": 2837.36, "end": 2837.88, "word": " بيقوم", "probability": 0.89697265625}, {"start": 2837.88, "end": 2838.3, "word": " بنفس", "probability": 0.98486328125}, {"start": 2838.3, "end": 2838.88, "word": " الوظيفة", "probability": 0.98955078125}, {"start": 2838.88, "end": 2839.02, "word": " اللى", "probability": 0.922119140625}, {"start": 2839.02, "end": 2839.24, "word": " هو", "probability": 0.9462890625}, {"start": 2839.24, "end": 2839.84, "word": " اسمه", "probability": 0.9361979166666666}, {"start": 2839.84, "end": 2840.84, "word": " hydrogen", "probability": 0.78662109375}, {"start": 2840.84, "end": 2842.4, "word": " peroxidase", "probability": 0.8096923828125}, {"start": 2842.4, "end": 2842.88, "word": " من", "probability": 0.9345703125}, {"start": 2842.88, "end": 2843.24, "word": " اسمه", "probability": 0.9619140625}, {"start": 2843.24, "end": 2843.68, "word": " hydrogen", "probability": 0.95166015625}, {"start": 2843.68, "end": 2844.46, "word": " peroxidase", "probability": 0.9288330078125}, {"start": 2844.46, "end": 2844.68, "word": " يعني", "probability": 0.94091796875}, {"start": 2844.68, "end": 2845.24, "word": " بيكسر", "probability": 0.90634765625}, {"start": 2845.24, "end": 2845.34, "word": " ال", "probability": 0.97265625}, {"start": 2845.34, "end": 2845.74, "word": " hydrogen", "probability": 0.66259765625}, {"start": 2845.74, "end": 2846.46, "word": " peroxide", "probability": 0.9267578125}, {"start": 2846.46, "end": 2847.48, "word": " هيشتغل", "probability": 0.90751953125}, {"start": 2847.48, "end": 2847.72, "word": " على", "probability": 0.94189453125}, {"start": 2847.72, "end": 2848.24, "word": " نصف", "probability": 0.8167317708333334}, {"start": 2848.24, "end": 2848.5, "word": " ال", "probability": 0.6875}, {"start": 2848.5, "end": 2849.04, "word": " substrate", "probability": 0.370361328125}, {"start": 2849.04, "end": 2849.3, "word": " اللى", "probability": 0.849609375}, {"start": 2849.3, "end": 2849.44, "word": " هى", "probability": 0.723388671875}, {"start": 2849.44, "end": 2849.58, "word": " ال", "probability": 0.78955078125}, {"start": 2849.58, "end": 2850.56, "word": " H2O2", "probability": 0.8653564453125}, {"start": 2850.56, "end": 2850.9, "word": " لكن", "probability": 0.88330078125}, {"start": 2850.9, "end": 2852.06, "word": " هيعطينى", "probability": 0.7541015625}, {"start": 2852.06, "end": 2853.04, "word": " جزيئان", "probability": 0.9015625}, {"start": 2853.04, "end": 2853.44, "word": " ميه", "probability": 0.8732096354166666}, {"start": 2853.44, "end": 2854.0, "word": " هيعطينى", "probability": 0.88583984375}, {"start": 2854.0, "end": 2854.4, "word": " two", "probability": 0.455078125}, {"start": 2854.4, "end": 2855.78, "word": " جزيئان", "probability": 0.97802734375}, {"start": 2855.78, "end": 2856.88, "word": " H2O2", "probability": 0.9830322265625}], "temperature": 1.0}, {"id": 101, "seek": 287452, "start": 2857.32, "end": 2874.52, "text": "النواتج هتختلف هيعطيني جزيئان ميا مش هيعطيني O2 فبالتالي مش هقدر انا استدل على التفاعل هذا زي ال Catalase Test هذا بالنسبة لفحص Catalase Test خلصناه هنتقل ل ال Coagulase Test", "tokens": [6027, 1863, 2407, 9307, 7435, 8032, 2655, 46456, 46538, 39896, 3615, 9566, 1829, 22653, 10874, 11622, 1829, 19986, 7649, 3714, 25528, 37893, 8032, 40228, 9566, 1829, 22653, 422, 17, 6156, 3555, 6027, 2655, 6027, 1829, 37893, 8032, 28543, 2288, 1975, 8315, 44713, 3215, 1211, 15844, 16712, 5172, 995, 30241, 23758, 30767, 1829, 2423, 24994, 651, 9279, 23758, 20666, 1863, 35457, 3660, 5296, 5172, 5016, 9381, 24994, 651, 9279, 16490, 1211, 9381, 8315, 3224, 8032, 29399, 4587, 1211, 5296, 2423, 3066, 559, 425, 651, 9279], "avg_logprob": -0.16277574020273544, "compression_ratio": 1.5942857142857143, "no_speech_prob": 5.960464477539063e-08, "words": [{"start": 2857.32, "end": 2858.06, "word": "النواتج", "probability": 0.8841796875}, {"start": 2858.06, "end": 2858.74, "word": " هتختلف", "probability": 0.9373779296875}, {"start": 2858.74, "end": 2859.32, "word": " هيعطيني", "probability": 0.83310546875}, {"start": 2859.32, "end": 2859.8, "word": " جزيئان", "probability": 0.89267578125}, {"start": 2859.8, "end": 2860.06, "word": " ميا", "probability": 0.6572265625}, {"start": 2860.06, "end": 2860.18, "word": " مش", "probability": 0.71435546875}, {"start": 2860.18, "end": 2860.78, "word": " هيعطيني", "probability": 0.774560546875}, {"start": 2860.78, "end": 2861.24, "word": " O2", "probability": 0.78662109375}, {"start": 2861.24, "end": 2861.86, "word": " فبالتالي", "probability": 0.97412109375}, {"start": 2861.86, "end": 2862.06, "word": " مش", "probability": 0.94970703125}, {"start": 2862.06, "end": 2862.52, "word": " هقدر", "probability": 0.9874674479166666}, {"start": 2862.52, "end": 2862.74, "word": " انا", "probability": 0.677978515625}, {"start": 2862.74, "end": 2863.26, "word": " استدل", "probability": 0.9036458333333334}, {"start": 2863.26, "end": 2863.44, "word": " على", "probability": 0.9677734375}, {"start": 2863.44, "end": 2864.18, "word": " التفاعل", "probability": 0.95361328125}, {"start": 2864.18, "end": 2864.58, "word": " هذا", "probability": 0.8828125}, {"start": 2864.58, "end": 2865.32, "word": " زي", "probability": 0.968017578125}, {"start": 2865.32, "end": 2865.48, "word": " ال", "probability": 0.90869140625}, {"start": 2865.48, "end": 2866.1, "word": " Catalase", "probability": 0.6446533203125}, {"start": 2866.1, "end": 2866.66, "word": " Test", "probability": 0.6298828125}, {"start": 2866.66, "end": 2867.6, "word": " هذا", "probability": 0.8017578125}, {"start": 2867.6, "end": 2868.34, "word": " بالنسبة", "probability": 0.9906005859375}, {"start": 2868.34, "end": 2870.1, "word": " لفحص", "probability": 0.8974609375}, {"start": 2870.1, "end": 2870.88, "word": " Catalase", "probability": 0.896728515625}, {"start": 2870.88, "end": 2871.26, "word": " Test", "probability": 0.86767578125}, {"start": 2871.26, "end": 2872.02, "word": " خلصناه", "probability": 0.9537109375}, {"start": 2872.02, "end": 2872.7, "word": " هنتقل", "probability": 0.9140625}, {"start": 2872.7, "end": 2873.0, "word": " ل", "probability": 0.69775390625}, {"start": 2873.0, "end": 2873.16, "word": " ال", "probability": 0.1943359375}, {"start": 2873.16, "end": 2874.1, "word": " Coagulase", "probability": 0.887939453125}, {"start": 2874.1, "end": 2874.52, "word": " Test", "probability": 0.90234375}], "temperature": 1.0}], "language": "ar", "language_probability": 1.0, "duration": 2877.138125, "duration_after_vad": 2393.21562499999}
\ No newline at end of file
diff --git a/PL9fwy3NUQKwZWgAb_V1ATlRRsH-6i3cPC/ZJy0jbwSDlU.srt b/PL9fwy3NUQKwZWgAb_V1ATlRRsH-6i3cPC/ZJy0jbwSDlU.srt
new file mode 100644
index 0000000000000000000000000000000000000000..fc91295a88d8ee6381c2b9970be7de43c1383ddf
--- /dev/null
+++ b/PL9fwy3NUQKwZWgAb_V1ATlRRsH-6i3cPC/ZJy0jbwSDlU.srt
@@ -0,0 +1,1625 @@
+1
+00:00:03,050 --> 00:00:06,790
+السلام عليكم ورحمة الله وبركاته يعطيكم العافية
+
+2
+00:00:06,790 --> 00:00:10,390
+جميعًا في هذا اللقاء هنستكمل الـ biochemical test
+
+3
+00:00:10,390 --> 00:00:15,550
+هنتكلم عن اليورياز واختبار اختزال النترات
+
+4
+00:00:15,550 --> 00:00:21,090
+هنبدأ باليورياز test، من اسمه يورياز test يبقى الـ
+
+5
+00:00:21,090 --> 00:00:26,050
+enzyme اللي هكشف عنه في هذا الفحص، اليورياز يعتبر
+
+6
+00:00:26,050 --> 00:00:30,850
+إنزيمًا، هيشتغل على اليوريا as substrate، يبقى الـ
+
+7
+00:00:30,850 --> 00:00:36,870
+substrate في هذا الفحص اليوريا، إنزيم اليورياز هيكسر
+
+8
+00:00:36,870 --> 00:00:40,570
+اليوريا، اليوريا قلنا عبارة عن toxic product
+
+9
+00:00:40,570 --> 00:00:45,350
+اليورياز هيعمل breakdown لليوريا، هنتخلص من
+
+10
+00:00:45,350 --> 00:00:50,410
+اليوريا عن طريق إنزيم اليورياز اللي بتفرزه بعض
+
+11
+00:00:50,410 --> 00:00:55,950
+أنواع البكتيريا، بنفس الوقت اليوريا تعتبر non-
+
+12
+00:00:55,950 --> 00:01:01,560
+carbohydrate carbon source، الكربوهيدرات هو المصدر
+
+13
+00:01:01,560 --> 00:01:06,640
+الأول لحصول على الـ carbon source اللازم لنمو
+
+14
+00:01:06,640 --> 00:01:11,460
+البكتيريا، في حال ما كان عندي كربوهيدرات أو كان
+
+15
+00:01:11,460 --> 00:01:15,320
+موجود وخلص الكربوهيدرات، البكتيريا هتلجأ لمصادر
+
+16
+00:01:15,320 --> 00:01:20,560
+أخرى حتى تحصل على الـ carbon source اللازم لنمو
+
+17
+00:01:20,560 --> 00:01:25,340
+البكتيريا، من هذه المصادر السيترات اللي اتكلمنا عنه
+
+18
+00:01:25,340 --> 00:01:29,900
+في المحاضرة السابقة، وعرفنا أن بعض أنواع البكتيريا
+
+19
+00:01:29,900 --> 00:01:33,740
+عندها القدرة على استهلاك السيترات، عندها إنزيمات
+
+20
+00:01:33,740 --> 00:01:38,320
+بتكسر السيترات، بالنهاية بحصل على الـ bio fat اللي
+
+21
+00:01:38,320 --> 00:01:44,620
+يعتبر كـ carbon source، كمصدر للكربون اللازم لنمو
+
+22
+00:01:44,620 --> 00:01:48,380
+البكتيريا، برضه اليوريا تعتبر non-carbohydrate
+
+23
+00:01:48,380 --> 00:01:52,540
+carbon source، اليوريا إذا هيتكسر اليوريا، هحصل على
+
+24
+00:01:52,540 --> 00:01:57,460
+أمونيا، الأمونيا تعتبر كـ nitrogen source، زائد CO2
+
+25
+00:01:57,460 --> 00:02:02,600
+يعتبر كـ carbon source لازم لنمو البكتيريا، طيب أي
+
+26
+00:02:02,600 --> 00:02:08,700
+تفاعل بيصير عشان أستدل عليه؟ باستدل من خلال الـ
+
+27
+00:02:08,700 --> 00:02:14,540
+product، نواتج التفاعل، فإما بضيف reagent، هذا الـ
+
+28
+00:02:14,540 --> 00:02:19,840
+reagent بيرتبط مع إحدى نواتج التفاعل وبيعطيني لون
+
+29
+00:02:19,840 --> 00:02:25,340
+،هكذا بعرف أنه التفاعل صار، أو الـ product بيكون عبارة
+
+30
+00:02:25,340 --> 00:02:30,940
+عن مركب حمضي أو قاعدي، فبالتالي بيتغير الـ pH، فبضيف
+
+31
+00:02:30,940 --> 00:02:35,740
+pH indicator في الميديا عشان يدل على حدوث التفاعل
+
+32
+00:02:35,740 --> 00:02:41,260
+في هذا الفحص عندي alkaline product، الـ NH3، الأمونيا
+
+33
+00:02:41,260 --> 00:02:46,080
+فبالتالي هضيف pH indicator في الميديا عشان أعرف هل
+
+34
+00:02:46,080 --> 00:02:50,080
+صار التفاعل ولا لأ، هل في عندي إنزيم اليورياز ولا
+
+35
+00:02:50,080 --> 00:02:57,050
+لأ، بالنسبة للـ Procedure، هستخدم يوريا أجار، الـ يوريا
+
+36
+00:02:57,050 --> 00:03:03,950
+أجار بصبها في slant، أو ممكن أنها تكون الـ يوريا
+
+37
+00:03:03,950 --> 00:03:08,590
+أبرث، أو ممكن تكون أجار بلات، يبقى فحص اليوريا ممكن
+
+38
+00:03:08,590 --> 00:03:12,290
+يكون slant أو أجار بلات أو أبرث، لكن الـ most
+
+39
+00:03:12,290 --> 00:03:17,840
+common بيكون slant، بعقم اليوريا أجار في الـ
+
+40
+00:03:17,840 --> 00:03:21,960
+filtration وليس في الـ autoclave، لأن اليوريا بتتكسر
+
+41
+00:03:21,960 --> 00:03:26,160
+في الحرارة، فبيتم تعقيم اليوريا أجار في الـ
+
+42
+00:03:26,160 --> 00:03:30,320
+filtration، مكونات الـ media، كأي media، في عندي
+
+43
+00:03:30,320 --> 00:03:34,040
+normal saline، الـ normal saline عشان يحافظ على الـ
+
+44
+00:03:34,040 --> 00:03:37,900
+osmotic pressure، في عندي mono potassium phosphate،
+
+45
+00:03:37,900 --> 00:03:43,040
+هذا يعتبر buffer، هيحافظ على ثبات الـ pH
+
+46
+00:03:43,910 --> 00:03:48,730
+البيبتون يعتبر بروتين، كمصدر لـ nitrogen source، و
+
+47
+00:03:48,730 --> 00:03:53,210
+carbon source لازم لنمو البكتيريا، لكن البيبتون
+
+48
+00:03:53,210 --> 00:03:57,510
+الموجود في ميديا اليوريا أجار موجود بكمية قليلة، 1
+
+49
+00:03:57,510 --> 00:04:03,010
+gram per liter، في عندي اليوريا، اليوريا هي الـ
+
+50
+00:04:03,010 --> 00:04:06,990
+substrate اللي هيشتغل عليها إنزيم اليورياز، موجودة
+
+51
+00:04:06,990 --> 00:04:11,980
+بكمية كبيرة، 20 gram per liter، الـ Urea هي الـ
+
+52
+00:04:11,980 --> 00:04:15,160
+substrate اللي بيشتغل عليها الإنزيم، هو بالتالي هتكون
+
+53
+00:04:15,160 --> 00:04:20,740
+الـ CO2 اللي يعتبر كـ carbon source لازم لنمو
+
+54
+00:04:20,740 --> 00:04:25,000
+البكتيريا، الـ Dextrose اللي هو الجلوكوز يعتبر كـ
+
+55
+00:04:25,000 --> 00:04:29,200
+energy source، كمصدر للطاقة، و carbon source برضه
+
+56
+00:04:29,200 --> 00:04:35,360
+موجود بكمية قليلة، حتى يعتبر carbon source لازم
+
+57
+00:04:35,360 --> 00:04:39,840
+لنمو البكتيريا، أكيد بما أنه ناتج التفاعل في عندي
+
+58
+00:04:39,840 --> 00:04:43,960
+alkaline product، فهضيف pH indicator عشان أكشف على
+
+59
+00:04:43,960 --> 00:04:49,300
+النواتج، الـ pH indicator هو الـ phenol red، الـ phenol
+
+60
+00:04:49,300 --> 00:04:53,160
+red، مر معنا قبل كده في فحص الـ fermentation، يبقى
+
+61
+00:04:53,160 --> 00:04:58,840
+عشان نحفظ فحص الـ fermentation وفحص اليوريا، كان الـ
+
+62
+00:04:58,840 --> 00:05:02,560
+pH indicator الموجود في الـ media هو
+
+63
+00:05:02,560 --> 00:05:08,710
+phenol red، في الـ acidic بيكون لونه
+
+64
+00:05:08,710 --> 00:05:12,370
+yellow، في الـ alkaline بيكون لونه red، في الـ neutral
+
+65
+00:05:12,370 --> 00:05:16,950
+بيكون لونه orange، هزرع البكتيريا اللي بدي أكشف
+
+66
+00:05:16,950 --> 00:05:21,390
+عندها إنزيم اليورياز ولا لأ، كيف بدي أنا أعمل، أو كيف
+
+67
+00:05:21,390 --> 00:05:25,330
+بدي أزرع الـ slant؟ بعمل stabbing للمنطقة اللي تحت
+
+68
+00:05:25,330 --> 00:05:31,570
+بعدين هطلع زي زجزاج، طبعًا هاعقم الفوهة قبل وبعد، بعد
+
+69
+00:05:31,570 --> 00:05:37,090
+هعمل incubation لمدة 24 ساعة، بعد الـ incubation هشوف
+
+70
+00:05:37,090 --> 00:05:42,710
+اللي هي الـ result، النتيجة، بقول لي: إذا لاحظت في
+
+71
+00:05:42,710 --> 00:05:46,090
+عندي growth with a change in color of media from
+
+72
+00:05:46,090 --> 00:05:49,530
+pink to red، هيكون في عندي نمو للبكتيريا، أشهر
+
+73
+00:05:49,530 --> 00:05:53,410
+شيء طبيعي، في عندي بيبتون كمصدر للنيتروجين والـ carbon
+
+74
+00:05:53,410 --> 00:05:56,790
+source بكمية قليلة، في عندي ديكستروز كمصدر للـ
+
+75
+00:05:56,790 --> 00:06:01,180
+carbon source بكمية قليلة، خلاص الـ Dextrose والـ
+
+76
+00:06:01,180 --> 00:06:05,200
+بيبتون، لأنه موجود في كمية قليلة، هتلجأ لمصادر
+
+77
+00:06:05,200 --> 00:06:09,060
+ثانية، اللي هي الـ non-carbohydrate carbon source
+
+78
+00:06:09,060 --> 00:06:15,380
+اليوريا، لأن عندها إنزيم اليورياز طبعًا، فهتكسر
+
+79
+00:06:15,380 --> 00:06:21,040
+اليوريا، وهحصل على CO2 زائد NH3، عندي carbon source
+
+80
+00:06:21,040 --> 00:06:25,280
+و nitrogen source، فبالتالي هتنمو البكتيريا، هيتغير
+
+81
+00:06:25,280 --> 00:06:28,340
+لون الـ media للون pink to red، لون الـ media الأصلي
+
+82
+00:06:28,340 --> 00:06:33,080
+بيكون لونه pink، لكن لون pink فاتح، هيتحول لون الـ
+
+83
+00:06:33,080 --> 00:06:38,720
+media للون فوشي، للون زهري غامق جدًا، فبالتالي هذه
+
+84
+00:06:38,720 --> 00:06:41,840
+النتيجة الـ positive، طيب ليش اتغير لون الـ media
+
+85
+00:06:41,840 --> 00:06:46,520
+للون الـ pink أو الـ red، اللون الفوشي الغامق، لأنه
+
+86
+00:06:46,520 --> 00:06:50,800
+عندي إنزيم اليورياز كسر اليوريا، نتج أمونيا،
+
+87
+00:06:50,800 --> 00:06:55,140
+الأمونيا alkaline product، الـ alkaline product طبعًا
+
+88
+00:06:55,140 --> 00:07:00,340
+عندي أنا pH indicator، phenol red، في حال كانت في
+
+89
+00:07:00,340 --> 00:07:05,340
+حال اللي هو الـ basic، هيتغير لون الـ pH indicator
+
+90
+00:07:05,340 --> 00:07:09,960
+للون الأحمر، نتيجة اللون الأحمر اللي هو تكون الـ
+
+91
+00:07:09,960 --> 00:07:13,720
+alkaline product، الأمونيا، هيرفع لي الـ pH، وهيتحول
+
+92
+00:07:13,720 --> 00:07:19,380
+لون الـ pH indicator للون الـ red، من الأمثلة على الـ
+
+93
+00:07:19,380 --> 00:07:22,080
+positive، النتيجة الـ positive، يورياز positive:
+
+94
+00:07:22,080 --> 00:07:26,220
+البروتيوس، الهيليكوباكتر، الكليبسلا، النيومونيا، الانتيرو-
+
+95
+00:07:26,220 --> 00:07:30,600
+باكتر، واليرسينيا، الانتيروكوليتكا، بالنسبة للنتيجة الـ
+
+96
+00:07:30,600 --> 00:07:36,280
+negative، بقول لي:
+
+97
+00:07:36,280 --> 00:07:40,860
+أن بيكون عندي growth، بيكون عندي نمو للبكتيريا
+
+98
+00:07:41,330 --> 00:07:44,670
+إذا بتتذكروا في فحص السيترات، كانت النتيجة النيجاتيف
+
+99
+00:07:44,670 --> 00:07:49,830
+كان عندي اللون الأخضر، هو لون الـ media الأصلي قبل
+
+100
+00:07:49,830 --> 00:07:54,510
+ما أنا أزرع uninoculated، بيكون لونه أخضر، وبيكون في
+
+101
+00:07:54,510 --> 00:07:58,430
+عندي growth مش موجود، في عندي نمو للبكتيريا مش
+
+102
+00:07:58,430 --> 00:08:02,550
+موجود، لكن في فحص اليوريا النتيجة النيجاتيف بيكون في عندي
+
+103
+00:08:02,550 --> 00:08:06,350
+growth، بيكون عندي نمو للبكتيريا، طيب ليش
+
+104
+00:08:06,350 --> 00:08:10,470
+هَنقُول إن النتيجة النيجاتيف، في عندي growth في فحص
+
+105
+00:08:10,470 --> 00:08:15,370
+السيترات، إذا بتتذكروا، ما كان في عندي مصدر آخر للـ
+
+106
+00:08:15,370 --> 00:08:18,710
+carbon غير السيترات، فبالتالي البكتيريا اللي عندها
+
+107
+00:08:18,710 --> 00:08:21,690
+القدرة على استهلاك السيترات فقط هي اللي هتنمو، كان
+
+108
+00:08:21,690 --> 00:08:26,190
+مصدر وحيد للـ carbon اللي هو السيترات، لكن في فحص
+
+109
+00:08:26,190 --> 00:08:29,650
+اليوريا، في ميديا اليوريا أجار، في عندي بيبتون، في
+
+110
+00:08:29,650 --> 00:08:33,130
+عندي ديكستروز، كلها مصادر للـ carbon وللـ nitrogen
+
+111
+00:08:33,130 --> 00:08:37,050
+لازمة لنمو البكتيريا، فبالتالي حتى لو البكتيريا ما
+
+112
+00:08:37,050 --> 00:08:41,040
+عندها القدرة إنها تكسر اليوريا، عندي بيبتون
+
+113
+00:08:41,040 --> 00:08:44,880
+وديكستروز موجود بكمية قليلة، هتقدر إنها تنمو
+
+114
+00:08:44,880 --> 00:08:49,220
+البكتيريا، هذا بالنسبة ليش إن النتيجة الـ negative
+
+115
+00:08:49,220 --> 00:08:52,900
+فيه بيكون فيه نمو with not change in color of
+
+116
+00:08:52,900 --> 00:08:57,580
+media، بيكون لون الـ media قلنا pink، لون زهري فاتح
+
+117
+00:08:57,580 --> 00:09:02,300
+أو بيقول ممكن يتحول لون الـ media للون الـ yellow،
+
+118
+00:09:02,300 --> 00:09:06,060
+ليش بتحول اللون للون الـ yellow؟ لأنه بيقولنا ممكن
+
+119
+00:09:06,060 --> 00:09:11,530
+البكتيريا تعمل fermentation للديكستروز، يتكون حمض،
+
+120
+00:09:11,530 --> 00:09:16,930
+ويتحول لون الـ pH للون الأصفر، يبقى ممكن يكون عندها
+
+121
+00:09:16,930 --> 00:09:20,350
+الـ negative لونه يلو، نتيجة اللي هو الـ
+
+122
+00:09:20,350 --> 00:09:23,810
+fermentation للديكستروز الموجود في الـ media،
+
+123
+00:09:24,200 --> 00:09:28,520
+هيتكون acidic product، فبالتالي هيقلل الـ pH، ويتحول
+
+124
+00:09:28,520 --> 00:09:33,480
+الـ pH indicator للون الأصفر، من الأمثلة على الـ
+
+125
+00:09:33,480 --> 00:09:38,980
+Urease Negative، السالمونيلا والشيجيلا يعتبروا Urease
+
+126
+00:09:38,980 --> 00:09:43,200
+Negative، بالنسبة
+
+127
+00:09:43,200 --> 00:09:50,810
+لهذا المكتوب، بعمل incubation لمدة 24 ساعة، two four
+
+128
+00:09:50,810 --> 00:09:55,490
+days، بقول لي ما أقدر أكتب النتيجة negative إلا بعد
+
+129
+00:09:55,490 --> 00:10:00,490
+أربعة أيام من الـ incubation، يبقى بطلع الـ tube بعد
+
+130
+00:10:00,490 --> 00:10:05,590
+24 ساعة، إذا طلعت negative، برجعها على الـ incubator
+
+131
+00:10:05,590 --> 00:10:10,430
+بعد أربعة أيام، إذا ضلت negative خلاص بسجل النتيجة
+
+132
+00:10:10,430 --> 00:10:14,970
+negative، لكن ما بسجل النتيجة negative قبل أربعة أيام،
+
+133
+00:10:14,970 --> 00:10:19,600
+ليش؟ why؟ هذا السؤال مهم جدًا، واللي في عندي بكتيريا
+
+134
+00:10:19,600 --> 00:10:24,800
+delayed urease positive، بتاخد وقت أطول، أكتر من 48
+
+135
+00:10:24,800 --> 00:10:30,560
+ساعة عشان تكسر اليوريا وتنتج الأمونيا وترفع الـ
+
+136
+00:10:30,560 --> 00:10:36,180
+pH، ويتحول لون الوسط للون الـ pink، عشان هيك بخليها لـ
+
+137
+00:10:36,180 --> 00:10:41,700
+أربعة أيام، في المقابل في عندي rapid urease positive،
+
+138
+00:10:41,700 --> 00:10:45,980
+هذه البكتيريا خلال ست ساعات، يعني قبل الـ 24 ساعة
+
+139
+00:10:45,980 --> 00:10:50,820
+بتحول لون الميديا للون الفوشي زي البروتيوس
+
+140
+00:10:50,820 --> 00:10:54,800
+والهيليكوباكتر، هدول يعتبروا rapid urease positive،
+
+141
+00:10:54,800 --> 00:10:58,700
+حتى أنا بلاحظ خلال الساعات الست الأولى إنه اتحول لون
+
+142
+00:10:58,700 --> 00:11:05,060
+الـ slant للون الفوشي، لكن بالنسبة للجزء اللي
+
+143
+00:11:05,060 --> 00:11:10,320
+تحت، ده بيكون لونه أصفر بعد 24 ساعة، كل tube بيصير
+
+144
+00:11:10,320 --> 00:11:15,310
+لونها فوشي، هذه بالنسبة ليش إن أنا مكتوب هنا بخليها
+
+145
+00:11:15,310 --> 00:11:20,270
+لأربعة أيام، لأن قلنا في عندي delayed urease positive،
+
+146
+00:11:20,270 --> 00:11:24,670
+في نقطة ثالثة مهمة بالنسبة لفحص اليوريا test،
+
+147
+00:11:24,670 --> 00:11:30,430
+بقول لي: في عندي في الـ media بيبتون، قلنا إن بعض
+
+148
+00:11:30,430 --> 00:11:34,710
+أنواع البكتيريا بتعمل deamination للبيبتون، مش يعني
+
+149
+00:11:34,710 --> 00:11:39,890
+deamination للبيبتون يعني هتنزع الأمونيا من
+
+150
+00:11:39,890 --> 00:11:44,120
+البيبتون، من البروتين، وهيرتفع، بما إنه انتزعت الـ
+
+151
+00:11:44,120 --> 00:11:47,020
+ammonia، طلعت الأمونيا، هيرتفع الـ pH، الأمونيا
+
+152
+00:11:47,020 --> 00:11:51,980
+اللي خلّاه product، هيتحول اللون، رفعت الـ pH، صار
+
+153
+00:11:51,980 --> 00:11:56,780
+basic، هيتحول لون الـ media للون الأحمر أو الـ
+
+154
+00:11:56,780 --> 00:12:00,780
+deep pink، طب السؤال هنا: كيف بدي أفرق إن اللون
+
+155
+00:12:00,780 --> 00:12:04,320
+الأحمر كان نتيجة تكسير اليوريا ولا البيبتون؟
+
+156
+00:12:04,320 --> 00:12:11,490
+بقول لي إن التفسير لهذا الموضوع: إن كمية البيبتون
+
+157
+00:12:11,490 --> 00:12:15,650
+الموجودة في الميديا قلنا 1 gram per liter، لكن
+
+158
+00:12:15,650 --> 00:12:20,590
+اليوريا كانت 20 gram per liter، يبقى كمية البيبتون
+
+159
+00:12:20,590 --> 00:12:25,130
+بالنسبة لليوريا قليلة جدًا، في عندي أنا كمان buffer
+
+160
+00:12:25,130 --> 00:12:30,030
+في الميديا، mono potassium phosphate، هذا الـ buffer
+
+161
+00:12:30,030 --> 00:12:33,910
+هيحافظ لي على ثبات البيئة، لو البكتيريا عملت
+
+162
+00:12:33,910 --> 00:12:37,050
+deamination للبيبتون اللي كميته قليلة بالنسبة
+
+163
+00:12:37,050 --> 00:12:41,780
+لليوريا، الـ buffer هيقدر يعادل هذا التغير في الـ pH،
+
+164
+00:12:41,780 --> 00:12:47,540
+لكن لو كانت نتيجة تكسير اليوريا، اليوريا الموجودة
+
+165
+00:12:47,540 --> 00:12:52,800
+في الـ media كتيرة، فبالتالي ما بيقدر الـ buffer يعمل
+
+166
+00:12:52,800 --> 00:12:57,820
+معادلة لها، فبكون فقط اللون الأحمر يكون من تكسير
+
+167
+00:12:57,820 --> 00:13:04,980
+اليوريا، هذا بالنسبة لأهم الأشياء اللي في فحص
+
+168
+00:13:04,980 --> 00:13:09,640
+اليورياز، بشكل ملخص، ممكن إحنا قلنا إني أزرع
+
+169
+00:13:09,640 --> 00:13:14,900
+في slant أو broth أو أجار بلات، نيجي نمر على الـ
+
+170
+00:13:14,900 --> 00:13:19,920
+slides، يورياز
+
+171
+00:1
+
+223
+00:17:10,110 --> 00:17:14,770
+some hemophilus species يعتبروا برضه يورياز بوزيتف
+
+224
+00:17:14,770 --> 00:17:18,810
+Proteus mirabilis is a major cause of human urinary
+
+225
+00:17:18,810 --> 00:17:22,910
+tract infection. هنا احنا قلنا أن البروتياس تعتبر
+
+226
+00:17:22,910 --> 00:17:27,870
+non-pathogen in stool لكن هنا بقول لي هي pathogen
+
+227
+00:17:27,870 --> 00:17:31,430
+في الـ urine. يبقى الـ Proteus mirabilis هي الـ major
+
+228
+00:17:31,430 --> 00:17:36,390
+cause of human urinary tract infection. عشان هيك لو
+
+229
+00:17:36,390 --> 00:17:42,670
+مريض بيه الـ UTI وكان سبب الـ UTI بروتياس
+
+230
+00:17:42,670 --> 00:17:49,090
+بنلاحظ أن رائحة الـ urine بتكون فيها رائحة اللي هي
+
+231
+00:17:49,090 --> 00:17:53,890
+رائحة الأمونيا نتيجة تكسير الـ urea بواسطة أنزيم
+
+232
+00:17:53,890 --> 00:17:57,590
+الـ urease لأن البروتياس urease positive، فهيكسر الـ
+
+233
+00:17:57,590 --> 00:18:02,230
+urea، هيطلع أمونيا، الرائحة اللي هي رائحة الأمونيا.
+
+234
+00:18:02,630 --> 00:18:07,490
+هذا بالنسبة لـ significance لفحص اليورياز عشان يفرق
+
+235
+00:18:07,490 --> 00:18:10,150
+بين الـ Salmonella والشيجيلا اللي يعتبروا يورياز negative
+
+236
+00:18:10,680 --> 00:18:15,640
+باثوجين في الستول عن اليورياز positive اللي هي الـ
+
+237
+00:18:15,640 --> 00:18:19,040
+non-pathogen اللي هي البروتياس. طب كيف بدي أفرق
+
+238
+00:18:19,040 --> 00:18:23,180
+السلمونيلا والشيجيلا عن بعض؟ لو طلعت يورياز
+
+239
+00:18:23,180 --> 00:18:27,620
+negative، بقول في فحص الـ motility وفحص الـ H2S.
+
+240
+00:18:27,620 --> 00:18:31,760
+السلمونيلا positive للفحسين: motile and H2S
+
+241
+00:18:31,760 --> 00:18:36,420
+positive. الشيجيلا non-motile and H2S negative.
+
+242
+00:18:36,950 --> 00:18:41,850
+البروتياس نفس السلمونيلا: موتايل زائد H2S positive.
+
+243
+00:18:41,850 --> 00:18:45,890
+طبعاً بفرق بينهم بفحص اللي هو اليورياز: البروتياس
+
+244
+00:18:45,890 --> 00:18:50,690
+positive والسلمونيلا negative لليورياز. هذا بالنسبة
+
+245
+00:18:50,690 --> 00:18:58,750
+لـ significance لفحص اليورياز.
+
+246
+00:18:58,750 --> 00:19:02,070
+Urease producing by some Enterobacteriaceae. اليورياز
+
+247
+00:19:02,070 --> 00:19:04,150
+positive. قلنا البروتياس، الكلبسيلة،
+
+248
+00:19:04,150 --> 00:19:08,260
+Enterobacteriaceae، Yersinia enterocolitica، الهيليكوبكتر
+
+249
+00:19:08,260 --> 00:19:12,520
+بيلوري هو أكسداز بوزيتف. قلنا الهيليكوبكتر زي الـ
+
+250
+00:19:12,520 --> 00:19:19,380
+... زي الـ Vibrio cholerae، زي الـ Aeromonas. هذول كانوا
+
+251
+00:19:19,380 --> 00:19:27,000
+أكسداز بوزيتف، لكن كانت الـ Pseudomonas أكسداز زيهم.
+
+252
+00:19:27,000 --> 00:19:31,960
+Pseudomonas أكسداز بوزيتف. الهيليكوبكتر، الـ Aeromonas،
+
+253
+00:19:31,960 --> 00:19:36,640
+الـ Vibrio. لكن الاختلاف بينها وبين Pseudomonas أنه الـ
+
+254
+00:19:36,640 --> 00:19:40,500
+Helicobacter بقول لي هي Glucose fermenter، لكن
+
+255
+00:19:40,500 --> 00:19:45,520
+الـ Pseudomonas كانت Non-glucose fermenter. الـ
+
+256
+00:19:45,520 --> 00:19:49,220
+Helicobacter قلنا أنه هي Urease positive، بكتيرية
+
+257
+00:19:49,220 --> 00:19:53,100
+تعتبر Rapid urease positive، خلال ست ساعات بتتحول
+
+258
+00:19:53,100 --> 00:19:57,180
+اللون للون بنكي. والبروتياس، الـ Helicobacter، و
+
+259
+00:19:57,180 --> 00:20:00,400
+البروتياس يعتبروا Rapid urease positive.
+
+260
+00:20:05,110 --> 00:20:07,530
+الـ principle عشان إني أنا أفرق اليورياز positive و
+
+261
+00:20:07,530 --> 00:20:10,150
+اليورياز negative، بستخدم اللي هي
+
+262
+00:20:10,150 --> 00:20:14,190
+Urea agar. قلنا بتحتوي على يوريا بنسبة 20 جرام
+
+263
+00:20:14,190 --> 00:20:20,750
+per liter. per liter. جيلاتين، بيبتون. هدول مصادر
+
+264
+00:20:20,750 --> 00:20:24,750
+يعتبروا الـ protein، مصادر الـ nitrogen والـ carbon
+
+265
+00:20:24,750 --> 00:20:28,250
+source. موجود بكمية قليلة. صوديوم كلورايت. قلنا
+
+266
+00:20:28,250 --> 00:20:31,770
+عشان يحافظ على الـ osmotic pressure. ديكستروز موجود
+
+267
+00:20:31,770 --> 00:20:36,460
+بكمية قليلة. قلنا كمصدر للكربون، مصدر للطاقة. في non
+
+268
+00:20:36,460 --> 00:20:40,620
+-red. هو الـ pH indicator. مونوبتاسيوم فوسفات. قلنا
+
+269
+00:20:40,620 --> 00:20:44,100
+اللي هو الـ buffer، اللي هيحفظ لي على التغير في الـ
+
+270
+00:20:44,100 --> 00:20:47,700
+pH. some bacteria can utilize urea as non-
+
+271
+00:20:47,700 --> 00:20:50,480
+carbohydrate carbon source. قلنا الـ urea تعتبر
+
+272
+00:20:50,480 --> 00:20:53,940
+non-carbohydrate carbon source. باستخدام أنزيم الـ
+
+273
+00:20:53,940 --> 00:20:59,420
+urease. هي أنزيم الـ urease. وهي الـ urea هي الرابطة
+
+274
+00:20:59,420 --> 00:21:03,410
+ما بين الكربون والنيتروجين. ما بين الكربون
+
+275
+00:21:03,410 --> 00:21:07,190
+والنيتروجين. هتيجي اليورياز، ويكسر هاي الرابطة،
+
+276
+00:21:07,190 --> 00:21:12,630
+ويتكون عندي أمونيا زائد CO2. Urease activity: the
+
+277
+00:21:12,630 --> 00:21:15,890
+urea is detected by growing bacteria in media
+
+278
+00:21:15,890 --> 00:21:20,430
+containing urea. اليوريا هتكون كـ substrate، وبستخدم pH
+
+279
+00:21:20,430 --> 00:21:25,130
+indicator، اللي هو الـ phenol red. لو اليوريا اتكسرت
+
+280
+00:21:25,130 --> 00:21:29,690
+بواسطة أنزيم اليورياز، الأمونيا هتكون ناتج من نواتج
+
+281
+00:21:29,690 --> 00:21:36,530
+عملية التكسير، فبالتالي هيعملي الوسط alkaline، هيرفع
+
+282
+00:21:36,530 --> 00:21:40,810
+الـ pH، فبالتالي الـ indicator هيتحول لونه للون
+
+283
+00:21:40,810 --> 00:21:47,150
+الأحمر to deep pink، أو أنه هيتحول للون زي الـ
+
+284
+00:21:47,150 --> 00:21:51,750
+violet. هذا هو الـ positive لفحص اليوريا. Dextrose
+
+285
+00:21:51,750 --> 00:21:54,790
+قلنا موجود بكمية قليلة في الـ media، فبالتالي
+
+286
+00:21:54,790 --> 00:22:01,130
+البكتيريا هتروح تلجأ لمصدر ثاني لـ carbon source، أو
+
+287
+00:22:01,130 --> 00:22:05,290
+أنها تتوقف عن النمو في حال ما كان في عندها أنزيم
+
+288
+00:22:05,290 --> 00:22:10,870
+اليورياز. أكيد هتتوقف عن النمو، لأن البيبتون و
+
+289
+00:22:10,870 --> 00:22:14,630
+الديكستروز اللي يعتبروا كمصدر الكاربون اللازم لنمو
+
+290
+00:22:14,630 --> 00:22:19,410
+البكتيريا، خلص موجود بكمية قليلة، هتلجأ لليورياز
+
+291
+00:22:19,410 --> 00:22:23,470
+في حال ما كان عندها يورياز. أكيد هتتوقف عن النمو
+
+292
+00:22:23,470 --> 00:22:27,790
+في أي حال ما عندها يورياز. البكتيريا كانت يورياز
+
+293
+00:22:27,790 --> 00:22:31,930
+positive، أكيد هتلجأ لمصدر اليوريا عشان تحصل على
+
+294
+00:22:31,930 --> 00:22:36,790
+carbon source. نلاحظ
+
+295
+00:22:36,790 --> 00:22:40,610
+في الصورة أن في الـ non-red، في البيئة في الـ acidic بيكون
+
+296
+00:22:40,610 --> 00:22:45,270
+لونه yellow، لكن في الـ basic بيكون لونه فوشي. هيك
+
+297
+00:22:45,270 --> 00:22:49,090
+بيكون النتيجة الـ positive. هذا اللون الفوشي في حال
+
+298
+00:22:49,090 --> 00:22:50,630
+كان عندي يورياز positive.
+
+299
+00:22:53,640 --> 00:22:58,600
+الطريقة: إني أنا بزرع الـ slant في الـ media اليوريا
+
+300
+00:22:58,600 --> 00:23:02,400
+بالـ test organism، بالـ organism اللي بدي أكشف عنه.
+
+301
+00:23:02,400 --> 00:23:07,600
+هعمل incubation لمدة 24 ساعة لـ 4 days. وعرفنا ليش
+
+302
+00:23:07,600 --> 00:23:13,040
+أنا بدي أخليها لمدة 4 أيام، لأن في عندي delay
+
+303
+00:23:13,040 --> 00:23:19,560
+urease positive بكتيريا. النتيجة بشوفها بعد 48 ساعة.
+
+304
+00:23:19,560 --> 00:23:22,840
+هي: some bacteria have a delay urease reaction that
+
+305
+00:23:22,840 --> 00:23:27,180
+may require an incubation period longer than أكثر
+
+306
+00:23:27,180 --> 00:23:31,760
+من 48 ساعة. النتيجة الـ positive بشوف لون bright
+
+307
+00:23:31,760 --> 00:23:35,320
+pink color. هيتطور فيه الـ slant، بعدين ممكن أنه يمتد
+
+308
+00:23:35,320 --> 00:23:39,070
+في الـ media خلال أول تلت ساعات بشوفه في الـ slant. فلما
+
+309
+00:23:39,070 --> 00:23:43,150
+اتكمل الـ 24 ساعة بشوفه ممتد في كل الـ media. في الـ
+
+310
+00:23:43,150 --> 00:23:46,730
+negative، لا تغير في الـ original color of media. مش
+
+311
+00:23:46,730 --> 00:23:49,510
+هلاحظ أي تغير في لون الـ media، أو قلنا هيتحول
+
+312
+00:23:49,510 --> 00:23:56,550
+اللون للون الأصفر. هذه نتيجة الـ positive، والـ
+
+313
+00:23:56,550 --> 00:23:59,370
+negative. هنا احنا قلنا ممكن تكون breath، ممكن تكون
+
+314
+00:23:59,370 --> 00:24:02,510
+slant. هذه breath. النتيجة الـ negative: ما تغير لون
+
+315
+00:24:02,510 --> 00:24:05,310
+الـ media. النتيجة الـ positive: كان في عندي اللون
+
+316
+00:24:05,310 --> 00:24:09,240
+فوشي. high slant. النتيجة الـ positive: اللون فوشي في
+
+317
+00:24:09,240 --> 00:24:13,560
+كل tube. لكن الـ negative، ما تغير لون الـ media. الـ
+
+318
+00:24:13,560 --> 00:24:16,860
+Rapid positive. قلنا خلال أول ست ساعات بلاحظ أن
+
+319
+00:24:16,860 --> 00:24:21,840
+اللون الفوشي كان في الـ slant، لكن لو أنا خليتها أو
+
+320
+00:24:21,840 --> 00:24:26,760
+كملت لـ 24 ساعة، هلاحظ أنه هيمتد اللون الفوشي في كل
+
+321
+00:24:26,760 --> 00:24:30,920
+tube. High negative: هي ما تغير لون الـ media. دلوقتي
+
+322
+00:24:30,920 --> 00:24:35,300
+اللون اللي ما أخد no change on color of the media.
+
+323
+00:24:35,730 --> 00:24:39,390
+هذا بالنسبة لفحص اليورياز.
+
+324
+00:24:47,090 --> 00:24:51,570
+هنيجي هلأ للفحص الثاني اللي هو الـ Indole test. الـ
+
+325
+00:24:51,570 --> 00:24:55,430
+Indole test هيكشف لي على أنزيم التريبتوفاناز.
+
+326
+00:24:55,430 --> 00:25:00,550
+التريبتوفاناز يعتبر indo enzyme. من اسمه، هيشتغل على
+
+327
+00:25:00,550 --> 00:25:05,050
+التريبتوفان as substrate. التريبتوفان يعتبر amino
+
+328
+00:25:05,050 --> 00:25:09,790
+acid. الـ amino acid التريبتوفاناز هيكسر هذا الـ amino
+
+329
+00:25:09,790 --> 00:25:13,950
+acid عشان أحصل على carbon source و nitrogen source
+
+330
+00:25:13,950 --> 00:25:20,150
+لازم لنمو البكتيريا. التريبتوفان من مكوناته اندول و
+
+331
+00:25:20,150 --> 00:25:24,010
+بيروفات وأمونيا. هدول ثلاثة، لكن بيكونوا متصلين
+
+332
+00:25:24,010 --> 00:25:28,990
+ببعض، مع بعض، مرتبطين. في حال البكتيريا أفرزت ذات
+
+333
+00:25:28,990 --> 00:25:33,230
+إنزيم التريبتوفاناز، هتكسر التريبتوفان اللي هو الـ
+
+334
+00:25:33,230 --> 00:25:38,810
+substrate، هتعطيني اندول زائد بيروفات زائد أمونيا.
+
+335
+00:25:39,840 --> 00:25:43,400
+هدول الثلاثة هتعطيهم بشكل منفصل. كانوا هم مع بعض
+
+336
+00:25:43,400 --> 00:25:49,040
+مرتبطين ببعض. بـ... التريبتوفان. باسم التريبتوفان.
+
+337
+00:25:49,040 --> 00:25:51,640
+التريبتوفان عبارة عن اندول وبيروفات وأمونيا
+
+338
+00:25:51,640 --> 00:25:55,660
+مرتبطين ببعض. إجا أنزيم التريبتوفاناز، كسر
+
+339
+00:25:55,660 --> 00:25:59,880
+التريبتوفان، وأعطاني اندول زائد بيروفات زائد أمونيا.
+
+340
+00:25:59,880 --> 00:26:03,780
+طبعاً الأمونيا تستخدم كـ nitrogen source. البيروفات
+
+341
+00:26:03,780 --> 00:26:09,150
+تستخدم كمصدر للـ carbon source. هذا بالنسبة
+
+342
+00:26:09,150 --> 00:26:13,750
+للفحص الـ principle. كيف أنا بدي أشتغل هذا الفحص؟
+
+343
+00:26:13,750 --> 00:26:17,790
+بقول لي: بجيب media بتحتوي على الـ substrate الـ
+
+344
+00:26:17,790 --> 00:26:22,410
+tryptophan. ممكن أستخدم media اسمها الـ tryptone
+
+345
+00:26:22,410 --> 00:26:26,370
+broth. بتكون الـ broth بتحتوي على الـ tryptophan، الـ
+
+346
+00:26:26,370 --> 00:26:31,850
+substrate. أو ممكن أستخدم media اسمها SIM. الـ SIM
+
+347
+00:26:31,850 --> 00:26:38,150
+بتكون هاي الـ media slant، مش الـ broth. الـ SIM، أو
+
+348
+00:26:38,150 --> 00:26:44,670
+sorry، بتكون stab، مش slant. أنعدل هذه الكلمة. الـ S I
+
+349
+00:26:44,670 --> 00:26:48,150
+M. بتكون stab، زي اللي استخدمناها في فحص الـ motility.
+
+350
+00:26:48,150 --> 00:26:54,610
+الـ S بترمز أو بتكشف على فحص الـ H2S. الـ I بتكشف على
+
+351
+00:26:54,610 --> 00:26:59,670
+فحص الـ indole، والـ M بتكشف على فحص الـ motility. هذه
+
+352
+00:26:59,670 --> 00:27:03,190
+الـ media تعتبر single media multiple test. هنتعرف
+
+353
+00:27:03,190 --> 00:27:07,290
+في المحاضرة الجاية. إنّي أنا ميديا واحدة ممكن تكشف لي
+
+354
+00:27:07,290 --> 00:27:10,830
+على أكثر من فحص كيميائي. فهتكشف على الـ H2S، to
+
+355
+00:27:10,830 --> 00:27:13,790
+edge. وهتكشف على الـ motility، وهتكشف على الـ indole.
+
+356
+00:27:13,790 --> 00:27:18,450
+فممكن أستخدم هذه الميديا، أو ممكن أستخدم ميديا
+
+357
+00:27:18,450 --> 00:27:22,370
+tryptone broth، اللي فقط هتكشف لي على الـ indole. لكن
+
+358
+00:27:22,370 --> 00:27:26,330
+هذه الميديا هتكشف لي على تلاتة فحوصات، من ضمنهم الـ
+
+359
+00:27:26,330 --> 00:27:31,510
+indole. لو استخدمت tryptone broth، هاخد البكتيريا
+
+360
+00:27:31,510 --> 00:27:36,290
+اللي بدي أكشف هل عندها أنزيم التريبتوفاناز، وهعمل mix
+
+361
+00:27:36,290 --> 00:27:42,590
+في الـ broth. لو كانت SIM، هي أستاذ، فهعمل stabbing، طعنة
+
+362
+00:27:42,590 --> 00:27:48,470
+في المنتصف، وهشوف النتيجة بعد 24 ساعة. هعمل
+
+363
+00:27:48,470 --> 00:27:53,910
+incubation لمدة 24 ساعة. بقول لي إنه عشان أكشف عن
+
+364
+00:27:53,910 --> 00:27:59,070
+التفاعل، إنه صار ولا لأ، بدي أضيف reagent. هذا الـ
+
+365
+00:27:59,070 --> 00:28:03,210
+reagent هيرتبط مع واحد لو هتنجح التفاعل، هيرتبط مع
+
+366
+00:28:03,210 --> 00:28:08,570
+واحد من هدول، هيعطيني لون. هيك بعرف أن التفاعل صار
+
+367
+00:28:08,570 --> 00:28:12,970
+صار ولا لأ. بقول لي في فحص الـ indole test، بعد الـ
+
+368
+00:28:12,970 --> 00:28:18,290
+incubation، بتضيف Kovacs reagent. الـ Kovacs reagent
+
+369
+00:28:18,290 --> 00:28:23,200
+بقول لي يرتبط أو من مكونات الـ Kovacs reagent، الأول
+
+370
+00:28:23,200 --> 00:28:29,500
+خلّيني نحكي isoamyl alcohol، HCL، وفي مادة اللي هي
+
+371
+00:28:29,500 --> 00:28:36,700
+الـ p-dimethylaminobenzaldehyde (DMABA). هدول التلاتة مكونات من مكونات الـ Kovacs
+
+372
+00:28:36,700 --> 00:28:40,800
+reagent. هضيف الـ Kovacs reagent بعد 24 ساعة
+
+373
+00:28:40,800 --> 00:28:45,080
+incubation. هعمل شيك للـ tube، وهقرأ النتيجة
+
+374
+00:28:45,080 --> 00:28:50,050
+immediately. بقول لي لو اتكون حلقة لونها cherry red
+
+375
+00:28:50,050 --> 00:28:55,150
+ring، زي لون الكرز، يبقى هي positive للفحص
+
+376
+00:28:55,150 --> 00:28:58,970
+الاندول. عندها أنزيم التريبتوفاناز. مثال عليها
+
+377
+00:28:58,970 --> 00:29:05,150
+الـ E. coli. لو صار... لو ما صار عندي أي تغيير، ما
+
+378
+00:29:05,150 --> 00:29:09,470
+اتكون حلقة لونها زي لون الكرز، يبقى هي negative. زي
+
+379
+00:29:09,470 --> 00:29:13,510
+الـ Klebsiella والـ Enterobacter. طيب كيف صار
+
+380
+00:29:13,510 --> 00:29:18,030
+التفاعل؟ أو كيف اتكون الحلقة الحمراء؟ قلنا إنّ الـ
+
+381
+00:29:18,030 --> 00:29:22,050
+Kovacs reagent اللي أنا أضفته بيرتبط مع أحد نواتج
+
+382
+00:29:22,050 --> 00:29:26,210
+التفاعل، واللي الـ Kovacs reagent بيرتبط مع الـ indole.
+
+383
+00:29:26,210 --> 00:29:34,890
+مين بالتحديد اللي بيرتبط مع الـ indole من مكونات الـ
+
+384
+00:29:34,890 --> 00:29:38,990
+Kovacs reagent؟ من مكونات الـ Kovacs reagent اللي
+
+385
+00:29:38,990 --> 00:29:43,810
+بيرتبط مع الاندول اللي هو الـ DMABA. هيدا
+
+386
+00:29:43,810 --> 00:29:49,090
+هيرتبطوا اتنين هدول مع بعض تمام، ويكوّن لي complex.
+
+387
+00:29:49,090 --> 00:29:53,670
+هذا الـ complex هيرتبط مع بعضه. هيرسب في القاع. تمام؟ في
+
+388
+00:29:53,670 --> 00:29:59,090
+قاع اليوتيوب هيترسب. هيجي الـ HCL، هيرفع الـ complex
+
+389
+00:29:59,090 --> 00:30:03,070
+ويتكون حلقة في أعلى اليوتيوب. يبقى كذا فالتفاعل
+
+390
+00:30:03,
+
+445
+00:34:26,070 --> 00:34:31,050
+التريبتوفان D-aminase في حال البكتيريا ما عندها
+
+446
+00:34:31,050 --> 00:34:34,250
+إنزيم للتريبتوفان D-aminase، هتستخدم إنزيم آخر حتى
+
+447
+00:34:34,250 --> 00:34:37,110
+تستفيد من التريبتوفان D-aminase حتى تحصل على
+
+448
+00:34:37,110 --> 00:34:43,070
+carbon و nitrogen source. طيب، شو وظيفة الـ HCL-HAN؟
+
+449
+00:34:43,940 --> 00:34:51,720
+بيقول لنا أن الـ HCl-Han بيمنع ارتباط التريبتوفان مع
+
+450
+00:34:51,720 --> 00:34:56,240
+الـ FeCl3. هلأ لو البكتيريا ما عندها إنزيم
+
+451
+00:34:56,240 --> 00:35:01,300
+لتريبتوفان دي أميناز، هيضل التريبتوفان موجود في الـ
+
+452
+00:35:01,300 --> 00:35:07,280
+media. أضفت رأيي بخصوص أحد مكوناته، الـ FeCl3، بقول
+
+453
+00:35:07,280 --> 00:35:12,240
+لي أن الـ FeCl3 لو ما قلل الـ اندول بيروفات، يعني ما كان
+
+454
+00:35:12,240 --> 00:35:15,540
+هناك إنزيم لتريبتوفان دي أميناز موجود، اللي هيكسر
+
+455
+00:35:15,540 --> 00:35:19,120
+التريبتوفان، هيروح يرتبط مع الـ substrate مع
+
+456
+00:35:19,120 --> 00:35:23,940
+التريبتوفان ويعطيني لون أحمر أو لون brown shred.
+
+457
+00:35:23,940 --> 00:35:28,990
+فبالتالي هيعطيني false positive result. الـ HCl
+
+458
+00:35:28,990 --> 00:35:34,810
+هيمنع هذا الارتباط، فبالتالي هيمنع إنه يتكون لون
+
+459
+00:35:34,810 --> 00:35:38,990
+أحمر نتيجة ارتباط الـ FeCl3 و الـ Try و الـ
+
+460
+00:35:38,990 --> 00:35:42,830
+Tryptophan مع بعض، ويعطيني False Positive Result.
+
+461
+00:35:42,830 --> 00:35:48,150
+هذه وظيفة الـ HCl في الـ TDA Reagent. لكن الـ HCl
+
+462
+00:35:48,150 --> 00:35:52,970
+في الـ Covax Reagent، اللي هو يرفع الـ Complex اللي
+
+463
+00:35:52,970 --> 00:35:54,770
+اتكون في القاعدة الأعلى.
+
+464
+00:35:57,990 --> 00:36:02,030
+نِجي للسلايدات. الـ Indole test: the ability to degrade
+
+465
+00:36:02,030 --> 00:36:06,630
+amino acid to identifiable end product is often
+
+466
+00:36:06,630 --> 00:36:09,330
+used to differentiate among bacteria. بيقول لي هو
+
+467
+00:36:09,330 --> 00:36:13,450
+هذا الفحص هيكشف عن قدرة البكتيريا إنها تكسر الـ
+
+468
+00:36:13,450 --> 00:36:16,990
+amino acid، فبالتالي هيساعدني إني أفرق بين
+
+469
+00:36:16,990 --> 00:36:20,830
+البكتيريا. Tryptophan, for example, is hydrolyzed to
+
+470
+00:36:20,830 --> 00:36:24,850
+indol, pyruvic acid, and ammonia. قلنا من نواتج
+
+471
+00:36:24,850 --> 00:36:30,040
+التفاعل اندول، بيروفات، وأمونيا. هيتكسروا بواسطة
+
+472
+00:36:30,040 --> 00:36:34,160
+التريبتوفاناز. لأن التريبتوفان دي أميناز،  الاندول مع
+
+473
+00:36:34,160 --> 00:36:39,420
+البيروفات هيدلوا، والأمونيا هتكون لحالها. البيروفيك
+
+474
+00:36:39,420 --> 00:36:42,140
+أسيد قلنا إنه بيصير metabolized to produce large
+
+475
+00:36:42,140 --> 00:36:45,520
+amount of energy. هتكون مصدر للطاقة. الأمونيا
+
+476
+00:36:45,520 --> 00:36:50,500
+ستُستخدم، قلنا، عشان أعمل أو يتكون new amino acid
+
+477
+00:36:50,500 --> 00:36:55,670
+كمصدر للنيتروجين والكربون اللازم لتكوين الـ amino
+
+478
+00:36:55,670 --> 00:36:59,870
+acid. فبالتالي هيتكون البروتين اللازم لنمو البكتيريا.
+
+479
+00:36:59,870 --> 00:37:04,730
+الـ Indole ده اللي بأكشف عنه كيف؟ قلنا إنه بضيف Covax
+
+480
+00:37:04,730 --> 00:37:09,850
+reagent. بيتكون من para-dimethyl amino
+
+481
+00:37:09,850 --> 00:37:14,430
+benzaldehyde in alcohol. قلنا هذا هو الاختصار الـ B
+
+482
+00:37:15,620 --> 00:37:21,940
+الـ DMAB. هذا هو اختصارها. أحد مكونات الـ Covax
+
+483
+00:37:21,940 --> 00:37:26,740
+Reagent. بتقول لنا بترتبط بالاندول اللي هو أحد نواتج
+
+484
+00:37:26,740 --> 00:37:31,340
+التفاعل، هيعطيني اللي هو بالنهاية Complex. هذا الـ
+
+485
+00:37:31,340 --> 00:37:36,240
+Complex لونه أحمر، Cherry Red Ring. الـ FeCl3 هيعمل
+
+486
+00:37:36,240 --> 00:37:41,720
+على رفع هذا الراسب لفوق في Lithium. هيتكون Red
+
+487
+00:37:41,720 --> 00:37:45,990
+Color. البروسيجر: أنا هعمل incubation بيه لتريبتون
+
+488
+00:37:45,990 --> 00:37:51,010
+لتريبتون broth  SIM ميديا، المهم ميديا بتحتوي على
+
+489
+00:37:51,010 --> 00:37:55,150
+التريبتوفان اللي هيكون كـ substrate. هعمل incubation.
+
+490
+00:37:55,150 --> 00:37:58,810
+بعد الـ incubation، هضيف الـ Covax reagent. هعمل
+
+491
+00:37:58,810 --> 00:38:02,550
+shaking، و هقرأ immediately. هاي الاندول اللي هو
+
+492
+00:38:02,550 --> 00:38:08,540
+ناتج من نواتج عملية تكسير التريبتوفان. هذا الـ Covax
+
+493
+00:38:08,540 --> 00:38:12,180
+Reagent، قلنا مكوناته الـ p-dimethyl
+
+494
+00:38:12,180 --> 00:38:16,540
+aminobenzaldehyde و الـ HCl و الـ amyl alcohol.
+
+495
+00:38:16,540 --> 00:38:21,820
+بالأخر هيتكون عندي cherry red ring. النتيجة، قلنا
+
+496
+00:38:21,820 --> 00:38:24,860
+red ring in the top layer indicate the presence of
+
+497
+00:38:24,860 --> 00:38:29,840
+indol. لكن في حال غياب اللون، هذا بيدل إنه الاندول
+
+498
+00:38:29,840 --> 00:38:34,960
+ما اتكون، فبالتالي إنزيم التريبتوفاناز مش موجود في
+
+499
+00:38:34,960 --> 00:38:39,210
+البكتيريا. هذا الفحص  has significant، وبيقول لنا
+
+500
+00:38:39,210 --> 00:38:44,430
+بيميز الـ E. coli اللي هي positive لهذا الفحص. هيتكون
+
+501
+00:38:44,430 --> 00:38:49,070
+red ring في الـ top.  الـ from negative اللي negative
+
+502
+00:38:49,070 --> 00:38:52,430
+لهذا الفحص، هيكون سلبية، والـ interior بيكون ما فيه
+
+503
+00:38:52,430 --> 00:38:57,710
+أي red ring. قلنا الـ IMViC اللي هو الـ Indole و الـ
+
+504
+00:38:57,710 --> 00:39:01,270
+Methyl Red, Voges-Proskauer, Citrate. هدول الأربع فحوصات
+
+505
+00:39:01,270 --> 00:39:05,740
+بيستخدموا للتفريق بين coliform عن بعض. لو البكتيريا
+
+506
+00:39:05,740 --> 00:39:10,080
+طلعت lactose fermenter، انتروباكتيريا لكتوز فرمنتر،
+
+507
+00:39:10,080 --> 00:39:12,240
+عشان أفرق الـ Coliform، اللي هي الـ E. coli,
+
+508
+00:39:12,240 --> 00:39:17,160
+Citrobacter، و الـ Enterobacter و الـ E. coli، بستخدم أربع
+
+509
+00:39:17,160 --> 00:39:21,640
+فحوصات، اللي هم الـ Indole, Methyl Red, Voges-Proskauer و
+
+510
+00:39:21,640 --> 00:39:27,900
+السيترات، عشان أفرق الأربع genera هدول عن بعض.
+
+511
+00:39:31,190 --> 00:39:36,490
+هيك بنكون خلصنا الـ Indole test.  هنتقل لاخر فحص
+
+512
+00:39:36,490 --> 00:39:39,630
+كيميائي اللي هو الـ Nitrate Reduction test.
diff --git a/PL9fwy3NUQKwZWgAb_V1ATlRRsH-6i3cPC/ZJy0jbwSDlU_raw.json b/PL9fwy3NUQKwZWgAb_V1ATlRRsH-6i3cPC/ZJy0jbwSDlU_raw.json
new file mode 100644
index 0000000000000000000000000000000000000000..7bf1b83163e9920ba285fe44177b5f7af212d10b
--- /dev/null
+++ b/PL9fwy3NUQKwZWgAb_V1ATlRRsH-6i3cPC/ZJy0jbwSDlU_raw.json
@@ -0,0 +1 @@
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{"start": 846.87, "end": 847.19, "word": " and", "probability": 0.93359375}, {"start": 847.19, "end": 847.47, "word": " some", "probability": 0.900390625}, {"start": 847.47, "end": 848.13, "word": " homophilous", "probability": 0.516357421875}, {"start": 848.13, "end": 848.79, "word": " species", "probability": 0.7646484375}, {"start": 848.79, "end": 850.25, "word": " are", "probability": 0.8037109375}, {"start": 850.25, "end": 850.95, "word": " urease", "probability": 0.9353841145833334}, {"start": 850.95, "end": 851.61, "word": " positive", "probability": 0.93310546875}, {"start": 851.61, "end": 852.51, "word": " هذا", "probability": 0.72412109375}, {"start": 852.51, "end": 853.73, "word": " الفقرة", "probability": 0.8037109375}, {"start": 853.73, "end": 854.27, "word": " كلها", "probability": 0.9853515625}, {"start": 854.27, "end": 854.49, "word": " ال", "probability": 0.5693359375}, {"start": 854.49, "end": 855.07, "word": " significant", "probability": 0.4208984375}, {"start": 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"word": " بقوللي", "probability": 0.72705078125}], "temperature": 1.0}, {"id": 34, "seek": 88307, "start": 863.31, "end": 883.07, "text": "انه بستخدمه عشان افرق ان ما ال lactose fermenter قولنا عائلة ال enterobacteriaceae اللى هى تعتبر إجرام نيجاتيف بصلية اكسيداز نيجاتيف بنميها على المقنكى اصده هان ال differential media اللى هى المقنكى", "tokens": [7649, 3224, 4724, 14851, 9778, 40448, 3224, 6225, 8592, 7649, 1975, 5172, 2288, 4587, 16472, 19446, 2423, 34042, 541, 38300, 260, 12174, 12610, 8315, 6225, 16373, 37977, 2423, 465, 391, 996, 14125, 72, 617, 8346, 13672, 7578, 8032, 7578, 6055, 34268, 26890, 11933, 7435, 2288, 10943, 8717, 1829, 7435, 9307, 33911, 4724, 36520, 10632, 1975, 4117, 3794, 25708, 31377, 8717, 1829, 7435, 9307, 33911, 44945, 2304, 1829, 11296, 15844, 9673, 4587, 1863, 4117, 7578, 1975, 9381, 3215, 3224, 8032, 7649, 2423, 15756, 3021, 13672, 7578, 8032, 7578, 9673, 4587, 1863, 4117, 7578], "avg_logprob": -0.30745967229207355, "compression_ratio": 1.6269430051813472, "no_speech_prob": 8.481740951538086e-05, "words": [{"start": 863.31, "end": 863.77, "word": "انه", "probability": 0.3436279296875}, {"start": 863.77, "end": 864.57, "word": " بستخدمه", "probability": 0.9484375}, {"start": 864.57, "end": 864.95, "word": " عشان", "probability": 0.9807942708333334}, {"start": 864.95, "end": 865.65, "word": " افرق", "probability": 0.9248046875}, {"start": 865.65, "end": 865.95, "word": " ان", "probability": 0.703125}, {"start": 865.95, "end": 866.13, "word": " ما", "probability": 0.1435546875}, {"start": 866.13, "end": 866.29, "word": " ال", "probability": 0.64453125}, {"start": 866.29, "end": 866.83, "word": " lactose", "probability": 0.783935546875}, {"start": 866.83, "end": 867.71, "word": " fermenter", "probability": 0.7412109375}, {"start": 867.71, "end": 868.35, "word": " قولنا", "probability": 0.8338216145833334}, {"start": 868.35, "end": 868.75, "word": " عائلة", "probability": 0.7945963541666666}, {"start": 868.75, "end": 868.91, "word": " ال", 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" المقنكى", "probability": 0.613720703125}, {"start": 879.71, "end": 880.25, "word": " اصده", "probability": 0.67877197265625}, {"start": 880.25, "end": 880.53, "word": " هان", "probability": 0.6220703125}, {"start": 880.53, "end": 880.63, "word": " ال", "probability": 0.919921875}, {"start": 880.63, "end": 881.07, "word": " differential", "probability": 0.9072265625}, {"start": 881.07, "end": 881.71, "word": " media", "probability": 0.9453125}, {"start": 881.71, "end": 881.97, "word": " اللى", "probability": 0.98388671875}, {"start": 881.97, "end": 882.31, "word": " هى", "probability": 0.971435546875}, {"start": 882.31, "end": 883.07, "word": " المقنكى", "probability": 0.991015625}], "temperature": 1.0}, {"id": 35, "seek": 91406, "start": 884.74, "end": 914.06, "text": "المقنكي تعتبر differential media هو selective media selective media لأنه بتنمي فقط لجرام negative بكتيريا مابتنمي لجرام positive بكتيريا differential media لأنه بتميز اللكتاز عن ال non-lactose fermenter فلو نمت البكتيريا اللكتاز fermenter بتعطيها اللون ال pink لكن لو نمت البكتيريا ال non-lactose fermenter بتعطيها اللون ال colorless هلأ بقوللي", "tokens": [45340, 4587, 1863, 4117, 1829, 6055, 34268, 26890, 15756, 3021, 31439, 33930, 3021, 33930, 3021, 5296, 33456, 3224, 39894, 1863, 2304, 1829, 6156, 47432, 5296, 7435, 2288, 10943, 3671, 4724, 4117, 2655, 13546, 25528, 3714, 16758, 2655, 1863, 2304, 1829, 5296, 7435, 2288, 10943, 3353, 4724, 4117, 2655, 13546, 25528, 15756, 3021, 5296, 33456, 3224, 39894, 2304, 1829, 11622, 13672, 4117, 2655, 31377, 18871, 2423, 2107, 12, 75, 578, 541, 38300, 260, 6156, 1211, 2407, 8717, 2304, 2655, 29739, 4117, 2655, 13546, 25528, 13672, 4117, 2655, 31377, 38300, 260, 39894, 3615, 9566, 1829, 11296, 13672, 11536, 2423, 7022, 44381, 45164, 8717, 2304, 2655, 29739, 4117, 2655, 13546, 25528, 2423, 2107, 12, 75, 578, 541, 38300, 260, 39894, 3615, 9566, 1829, 11296, 13672, 11536, 2423, 2017, 1832, 8032, 1211, 10721, 4724, 39648, 20292], "avg_logprob": 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0.8472900390625}, {"start": 897.02, "end": 897.9, "word": " اللكتاز", "probability": 0.7340087890625}, {"start": 897.9, "end": 898.84, "word": " عن", "probability": 0.97021484375}, {"start": 898.84, "end": 899.08, "word": " ال", "probability": 0.93701171875}, {"start": 899.08, "end": 899.3, "word": " non", "probability": 0.712890625}, {"start": 899.3, "end": 899.92, "word": "-lactose", "probability": 0.886474609375}, {"start": 899.92, "end": 900.58, "word": " fermenter", "probability": 0.850830078125}, {"start": 900.58, "end": 901.84, "word": " فلو", "probability": 0.97412109375}, {"start": 901.84, "end": 902.26, "word": " نمت", "probability": 0.98876953125}, {"start": 902.26, "end": 903.1, "word": " البكتيريا", "probability": 0.95849609375}, {"start": 903.1, "end": 903.78, "word": " اللكتاز", "probability": 0.95361328125}, {"start": 903.78, "end": 904.5, "word": " fermenter", "probability": 0.85791015625}, {"start": 904.5, "end": 905.26, "word": " بتعطيها", "probability": 0.97900390625}, {"start": 905.26, "end": 905.68, "word": " اللون", "probability": 0.942626953125}, {"start": 905.68, "end": 905.88, "word": " ال", "probability": 0.712890625}, {"start": 905.88, "end": 906.2, "word": " pink", "probability": 0.89990234375}, {"start": 906.2, "end": 906.76, "word": " لكن", "probability": 0.8515625}, {"start": 906.76, "end": 907.04, "word": " لو", "probability": 0.96630859375}, {"start": 907.04, "end": 907.36, "word": " نمت", "probability": 0.9892578125}, {"start": 907.36, "end": 908.04, "word": " البكتيريا", "probability": 0.98369140625}, {"start": 908.04, "end": 908.56, "word": " ال", "probability": 0.78515625}, {"start": 908.56, "end": 908.84, "word": " non", "probability": 0.9208984375}, {"start": 908.84, "end": 909.44, "word": "-lactose", "probability": 0.928955078125}, {"start": 909.44, "end": 910.0, "word": " fermenter", "probability": 0.958251953125}, {"start": 910.0, "end": 910.76, "word": " بتعطيها", "probability": 0.9931640625}, {"start": 910.76, 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الشيجيلا عن بعض لو طلعت يورياز negative بقول في فحص ال motility و فحص ال H2S السلمونيلا positive للفحسين motile and H2S positive الشيجيلا non motile and H2S negative", "tokens": [3555, 5718, 104, 29245, 9957, 8978, 2423, 14851, 12610, 18871, 45595, 13063, 1829, 31377, 3353, 13672, 7578, 8032, 7578, 2423, 2107, 3100, 8799, 13672, 7578, 8032, 7578, 2423, 26890, 35473, 1829, 31377, 23032, 3555, 9122, 33911, 11778, 1829, 1975, 5172, 2288, 4587, 21136, 19528, 11536, 1829, 15040, 4032, 25124, 1829, 7435, 1829, 15040, 18871, 45030, 11242, 45164, 23032, 1211, 34268, 7251, 13063, 1829, 31377, 3671, 4724, 39648, 8978, 6156, 5016, 9381, 2423, 2184, 1140, 4032, 6156, 5016, 9381, 2423, 389, 17, 50, 21136, 19528, 11536, 1829, 15040, 3353, 24976, 5172, 5016, 3794, 9957, 2184, 794, 293, 389, 17, 50, 3353, 25124, 1829, 7435, 1829, 15040, 2107, 2184, 794, 293, 389, 17, 50, 3671], "avg_logprob": -0.24629933870675272, "compression_ratio": 1.885321100917431, "no_speech_prob": 3.5762786865234375e-06, 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ممتد في كل ال media في ال negative لا تغير في ال original color of media مش هلاحظ اي تغير في لون ال media او قولنا هيتحول اللون للون الأصفر هذه نتيجة ال positive و ال negative هان احنا قولنا ممكن تكون breath ممكن تكون slant هذه breath النتيجة ال negative ما تغير لون ال media النتيجة ال positive كان في عندي اللون فوشي", "tokens": [5172, 1211, 15042, 1975, 2655, 24793, 1211, 2423, 4022, 8608, 995, 27884, 4724, 8592, 38688, 3224, 3714, 2304, 2655, 3215, 8978, 28242, 2423, 3021, 8978, 2423, 3671, 20193, 6055, 17082, 13546, 8978, 2423, 3380, 2017, 295, 3021, 37893, 8032, 15040, 5016, 19913, 1975, 1829, 6055, 17082, 13546, 8978, 5296, 11536, 2423, 3021, 1975, 2407, 12174, 12610, 8315, 8032, 36081, 5016, 12610, 13672, 11536, 5296, 1211, 11536, 16247, 9381, 5172, 2288, 29538, 8717, 31371, 7435, 3660, 2423, 3353, 4032, 2423, 3671, 8032, 7649, 1975, 5016, 8315, 12174, 12610, 8315, 3714, 43020, 6055, 30544, 6045, 3714, 43020, 6055, 30544, 1061, 394, 29538, 6045, 28239, 31371, 7435, 3660, 2423, 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substrate هعمل incubation بعد ال incubation هضيف ال covax reagent هعمل shacking و هقرأ immediately هاي الإندول اللي هو ناتج من نواتش عملية تكسير لتريبتوفان", "tokens": [6027, 26890, 2407, 8608, 1829, 7435, 2288, 1975, 8315, 8032, 25957, 1211, 33345, 399, 4724, 1829, 3224, 5296, 2655, 16572, 3555, 2655, 2407, 5296, 2655, 16572, 3555, 2655, 11536, 2423, 26890, 2407, 12984, 25855, 6324, 3714, 1829, 16254, 995, 9673, 16095, 3714, 1829, 16254, 995, 39894, 33753, 45865, 15844, 5296, 2655, 16572, 3555, 2655, 38688, 7649, 13672, 1829, 39896, 30544, 9122, 27585, 8032, 25957, 1211, 33345, 399, 39182, 2423, 33345, 399, 8032, 11242, 33911, 2423, 598, 2757, 87, 26949, 317, 8032, 25957, 1211, 402, 14134, 4032, 8032, 4587, 2288, 10721, 4258, 8032, 47302, 33688, 41260, 12610, 13672, 1829, 31439, 8717, 9307, 7435, 9154, 8717, 2407, 9307, 8592, 6225, 42213, 10632, 6055, 4117, 3794, 13546, 5296, 2655, 16572, 3555, 2655, 38688, 7649], "avg_logprob": -0.2722848316685098, "compression_ratio": 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"probability": 0.7744140625}, {"start": 2269.01, "end": 2269.89, "word": " المهم", "probability": 0.97216796875}, {"start": 2269.89, "end": 2270.21, "word": " ميديا", "probability": 0.951416015625}, {"start": 2270.21, "end": 2270.71, "word": " بتحتوي", "probability": 0.876953125}, {"start": 2270.71, "end": 2271.01, "word": " على", "probability": 0.8173828125}, {"start": 2271.01, "end": 2271.91, "word": " لتريبتوفان", "probability": 0.9105747767857143}, {"start": 2271.91, "end": 2272.39, "word": " اللي", "probability": 0.794189453125}, {"start": 2272.39, "end": 2272.95, "word": " هيكون", "probability": 0.95263671875}, {"start": 2272.95, "end": 2273.21, "word": " ك", "probability": 0.93701171875}, {"start": 2273.21, "end": 2273.85, "word": " substrate", "probability": 0.55419921875}, {"start": 2273.85, "end": 2274.37, "word": " هعمل", "probability": 0.97705078125}, {"start": 2274.37, "end": 2275.15, "word": " incubation", "probability": 0.94189453125}, {"start": 2275.15, "end": 2276.09, 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HCL و الـ aminalcohol بالآخر هيتكوّن لعندي sherry red ring النتيجة قلنا red ring in the top layer indicate the presence of indol لكن في حال غياب اللون هذا بيدل إنه الindol ما اتكون فبالتالي إنزيم لتربطه فناز مش موجود في البكتيريا", "tokens": [3224, 15730, 2423, 39184, 3066, 2757, 87, 1300, 559, 317, 12174, 1211, 8315, 3714, 4117, 2407, 8315, 47395, 2423, 39184, 363, 12, 13595, 3293, 5088, 12, 7428, 996, 11368, 304, 1479, 3495, 1479, 4032, 2423, 39184, 389, 22458, 4032, 2423, 39184, 669, 2071, 7210, 20666, 148, 95, 34740, 8032, 36081, 4117, 2407, 11703, 1863, 5296, 3615, 1863, 16254, 402, 5318, 2182, 4875, 28239, 31371, 7435, 3660, 12174, 1211, 8315, 2182, 4875, 294, 264, 1192, 4583, 13330, 264, 6814, 295, 1016, 401, 44381, 8978, 11331, 6027, 32771, 1829, 16758, 13672, 11536, 23758, 4724, 25708, 1211, 36145, 3224, 2423, 471, 401, 19446, 1975, 2655, 30544, 6156, 3555, 6027, 2655, 6027, 1829, 36145, 11622, 32640, 5296, 2655, 2288, 3555, 9566, 3224, 6156, 1863, 31377, 37893, 3714, 29245, 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red ring قولنا ال MVEC اللي هو ال Indol و ال Methyl Red Vox Bicarb Citrate هدول الأربع فحوصات يستخدموا للتفريق ال coliform عن بعض", "tokens": [3224, 15730, 27188, 5016, 9381, 12588, 279, 4776, 46740, 4032, 4724, 39648, 8315, 4724, 32640, 1829, 11622, 2423, 462, 1173, 72, 13672, 1829, 39896, 3353, 46740, 15730, 27188, 5016, 9381, 8032, 36081, 30544, 2182, 4875, 8978, 2423, 1192, 490, 3671, 13672, 1829, 3671, 46740, 15730, 27188, 5016, 9381, 5296, 4117, 1211, 3555, 8608, 37977, 4032, 2423, 10636, 4724, 995, 4117, 2655, 2288, 19446, 8032, 2655, 30544, 1975, 1829, 2182, 4875, 12174, 12610, 8315, 2423, 376, 7540, 34, 13672, 1829, 31439, 2423, 2333, 401, 4032, 2423, 48602, 5088, 4477, 691, 5230, 363, 7953, 65, 18435, 4404, 8032, 3215, 12610, 16247, 25513, 3615, 6156, 5016, 2407, 9381, 9307, 7251, 14851, 9778, 40448, 14407, 24976, 2655, 5172, 16572, 4587, 2423, 1173, 8629, 18871, 45030, 11242], "avg_logprob": -0.3732215321160913, "compression_ratio": 1.6405693950177935, "no_speech_prob": 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"end": 2352.9, "word": " سيتروباكتر", "probability": 0.6862564086914062}, {"start": 2352.9, "end": 2353.02, "word": " و", "probability": 0.33447265625}, {"start": 2353.02, "end": 2353.92, "word": " الانتيروباكتر", "probability": 0.8648003472222222}, {"start": 2353.92, "end": 2354.54, "word": " و", "probability": 0.7255859375}, {"start": 2354.54, "end": 2355.16, "word": " ال", "probability": 0.77197265625}, {"start": 2355.16, "end": 2355.34, "word": " E", "probability": 0.268798828125}, {"start": 2355.34, "end": 2355.82, "word": ".coli", "probability": 0.681640625}, {"start": 2355.82, "end": 2356.52, "word": " بستخدم", "probability": 0.8702392578125}, {"start": 2356.52, "end": 2357.16, "word": " أربع", "probability": 0.83544921875}, {"start": 2357.16, "end": 2357.74, "word": " فحوصات", "probability": 0.96484375}, {"start": 2357.74, "end": 2357.86, "word": " اللي", "probability": 0.94091796875}, {"start": 2357.86, "end": 2358.14, "word": " هم", "probability": 0.840576171875}, {"start": 2358.14, "end": 2358.24, "word": " ال", "probability": 0.86865234375}, {"start": 2358.24, "end": 2358.72, "word": " Endol", "probability": 0.44158935546875}, {"start": 2358.72, "end": 2360.0, "word": " Red", "probability": 0.1375732421875}, {"start": 2360.0, "end": 2360.52, "word": " Vagas", "probability": 0.5565999348958334}, {"start": 2360.52, "end": 2360.94, "word": " Biscuit", "probability": 0.5564778645833334}, {"start": 2360.94, "end": 2361.64, "word": " و", "probability": 0.71630859375}, {"start": 2361.64, "end": 2362.44, "word": " السيترات", "probability": 0.787109375}, {"start": 2362.44, "end": 2362.76, "word": " عشان", "probability": 0.9918619791666666}, {"start": 2362.76, "end": 2363.44, "word": " أفرق", "probability": 0.882568359375}, {"start": 2363.44, "end": 2365.34, "word": " الأربع", "probability": 0.9645182291666666}, {"start": 2365.34, "end": 2366.12, "word": " الأربع", "probability": 0.74072265625}, {"start": 2366.12, "end": 2366.52, "word": " هدول", "probability": 0.8548177083333334}, {"start": 2366.52, "end": 2367.08, "word": " genus", "probability": 0.525634765625}, {"start": 2367.08, "end": 2367.36, "word": " عن", "probability": 0.96728515625}, {"start": 2367.36, "end": 2367.9, "word": " بعض", "probability": 0.996826171875}], "temperature": 1.0}, {"id": 93, "seek": 237963, "start": 2371.19, "end": 2379.63, "text": "هك بنكون خلصنا ال test Indole هنتقل لاخر فحص كيميائي اللي هو ال test Nitrate Reduction", "tokens": [3224, 4117, 44945, 30544, 16490, 1211, 9381, 8315, 2423, 1500, 2333, 4812, 8032, 29399, 4587, 1211, 5296, 47283, 2288, 6156, 5016, 9381, 9122, 32640, 1829, 16373, 1829, 13672, 1829, 31439, 2423, 1500, 37942, 4404, 4477, 27549], "avg_logprob": -0.35114018981521194, "compression_ratio": 1.1043478260869566, "no_speech_prob": 5.960464477539063e-08, "words": [{"start": 2371.19, "end": 2371.49, "word": "هك", "probability": 0.80078125}, {"start": 2371.49, "end": 2371.81, "word": " بنكون", "probability": 0.520782470703125}, {"start": 2371.81, "end": 2372.57, "word": " خلصنا", "probability": 0.98095703125}, {"start": 2372.57, "end": 2372.81, "word": " ال", "probability": 0.814453125}, {"start": 2372.81, "end": 2373.09, "word": " test", "probability": 0.4775390625}, {"start": 2373.09, "end": 2373.45, "word": " Indole", "probability": 0.30230712890625}, {"start": 2373.45, "end": 2374.81, "word": " هنتقل", "probability": 0.7828369140625}, {"start": 2374.81, "end": 2376.07, "word": " لاخر", "probability": 0.7693684895833334}, {"start": 2376.07, "end": 2376.49, "word": " فحص", "probability": 0.9583333333333334}, {"start": 2376.49, "end": 2377.13, "word": " كيميائي", "probability": 0.9373046875}, {"start": 2377.13, "end": 2377.31, "word": " اللي", "probability": 0.5439453125}, {"start": 2377.31, "end": 2377.75, "word": " هو", "probability": 0.9775390625}, {"start": 2377.75, "end": 2378.23, "word": " ال", "probability": 0.74560546875}, {"start": 2378.23, "end": 2378.45, "word": " test", "probability": 0.3486328125}, {"start": 2378.45, "end": 2378.93, "word": " Nitrate", "probability": 0.8251953125}, {"start": 2378.93, "end": 2379.63, "word": " Reduction", "probability": 0.7529296875}], "temperature": 1.0}], "language": "ar", "language_probability": 1.0, "duration": 2381.3456875, "duration_after_vad": 2322.7199999999916}
\ No newline at end of file
diff --git a/PL9fwy3NUQKwZWgAb_V1ATlRRsH-6i3cPC/ZJy0jbwSDlU_raw.srt b/PL9fwy3NUQKwZWgAb_V1ATlRRsH-6i3cPC/ZJy0jbwSDlU_raw.srt
new file mode 100644
index 0000000000000000000000000000000000000000..0ee791eeec3097f2162996837089052282377b8c
--- /dev/null
+++ b/PL9fwy3NUQKwZWgAb_V1ATlRRsH-6i3cPC/ZJy0jbwSDlU_raw.srt
@@ -0,0 +1,2052 @@
+1
+00:00:03,050 --> 00:00:06,790
+السلام عليكم ورحمة الله وبركاته يعطيكم العافية
+
+2
+00:00:06,790 --> 00:00:10,390
+جميعا في هذا اللقاء هنستكمل ال biochemical test
+
+3
+00:00:10,390 --> 00:00:15,550
+هنتكلم عن اليورياز اندوال ان نيترات reduction test
+
+4
+00:00:15,550 --> 00:00:21,090
+هنبدأ باليورياز test من اسمه يورياز test يبقى ال
+
+5
+00:00:21,090 --> 00:00:26,050
+enzyme اللي هكشف عنه في هذا الفحص اليورياز يعتبر
+
+6
+00:00:26,050 --> 00:00:30,850
+اندوانزايم هيشتغل على اليوريا as substrate يبقى ال
+
+7
+00:00:30,850 --> 00:00:36,870
+substrateفي هذا الفحص اليوريا انزيم اليورياز هيكسر
+
+8
+00:00:36,870 --> 00:00:40,570
+اليوريا اليوريا قلنا عبارة عن toxic product
+
+9
+00:00:40,570 --> 00:00:45,350
+اليورياز هيعمل breakdown ل اليوريا هنتخلص من
+
+10
+00:00:45,350 --> 00:00:50,410
+اليوريا عن طريق انزيم اليورياز اللى بتفرزه بعض
+
+11
+00:00:50,410 --> 00:00:55,950
+أنواع البكتيريا بنفس الوقت اليوريا تعتبر non
+
+12
+00:00:55,950 --> 00:01:01,560
+carbohydrate carbon sourceالكربوهيدريات هو المصدر
+
+13
+00:01:01,560 --> 00:01:06,640
+الأول لحصول على ال carbon source اللازم لنمو
+
+14
+00:01:06,640 --> 00:01:11,460
+البكتيريا في حال ما كان عندي كربوهيدريات أو كان
+
+15
+00:01:11,460 --> 00:01:15,320
+موجود وخلص الكربوهيدريات البكتيريا هتلجأ لمصادر
+
+16
+00:01:15,320 --> 00:01:20,560
+أخرى حتى تحصل على ال carbon source اللازم لنمو
+
+17
+00:01:20,560 --> 00:01:25,340
+البكتيريامن هذه المصادر السيترات اللى اتكلمنا عنه
+
+18
+00:01:25,340 --> 00:01:29,900
+في المحاضرة السابقة وعرفنا ان بعض أنواع البكتيريا
+
+19
+00:01:29,900 --> 00:01:33,740
+عندها القدرة على استهلاك السيترات عندها انزيمات
+
+20
+00:01:33,740 --> 00:01:38,320
+بتكسر السيترات بالنهاية بحصل على ال bio fat اللى
+
+21
+00:01:38,320 --> 00:01:44,620
+يعتبر كcarbon source كمصدر للكربون اللازم لنمو
+
+22
+00:01:44,620 --> 00:01:48,380
+البكتيريا برضه اليوريا تعتبر non carbohydrate
+
+23
+00:01:48,380 --> 00:01:52,540
+carbon source اليوريا إذا هيتكسر اليوريا هحصل على
+
+24
+00:01:52,540 --> 00:01:57,460
+أمانيا الأمانيا تعتبر ك nitrogen source زائد CO2
+
+25
+00:01:57,460 --> 00:02:02,600
+يعتبر ك carbon source لازم لنمو البكتيريا طيب أي
+
+26
+00:02:02,600 --> 00:02:08,700
+تفاعل بيصير عشان أستدل عليهبستدل من خلال ال
+
+27
+00:02:08,700 --> 00:02:14,540
+product نواتج التفاعل فإما بضيف reagent هذا ال
+
+28
+00:02:14,540 --> 00:02:19,840
+reagent بيرتبط مع أحدى نواتج التفاعل وبيعطيني لون
+
+29
+00:02:19,840 --> 00:02:25,340
+هك بعرف أنه التفاعل صار أو ال product بيكون عبارة
+
+30
+00:02:25,340 --> 00:02:30,940
+عن مركب حمضي أو قاعدي فبالتالي بيتغير ال pHفبضيف
+
+31
+00:02:30,940 --> 00:02:35,740
+BH Indicator في الميديا عشان يدلل على حدوث التفاعل
+
+32
+00:02:35,740 --> 00:02:41,260
+في هذا الفحص عندي alkaline product الـNH3 الأمونيا
+
+33
+00:02:41,260 --> 00:02:46,080
+فبالتالي هضيف BH Indicator في الميديا عشان أعرف هل
+
+34
+00:02:46,080 --> 00:02:50,080
+صار التفاعل ولا لأ هل في عندي أنزيم اليورياز ولا
+
+35
+00:02:50,080 --> 00:02:57,050
+لأ بالنسبة لـ Procedureهستخدم يوريا أجار ال يوريا
+
+36
+00:02:57,050 --> 00:03:03,950
+أجار بصبها ب إسلانت أو ممكن أنها تكون ال يوريا
+
+37
+00:03:03,950 --> 00:03:08,590
+إبرث أو ممكن تكون أجار بلات يبقى فحص ال يوريا ممكن
+
+38
+00:03:08,590 --> 00:03:12,290
+يكون إسلانت أو أجار بلات أو إبرث لكن ال most
+
+39
+00:03:12,290 --> 00:03:17,840
+common بكون إسلانتبعقم اليوريا أجار في ال
+
+40
+00:03:17,840 --> 00:03:21,960
+filtration وليس في ال autoclave لأن اليوريا بتتكسر
+
+41
+00:03:21,960 --> 00:03:26,160
+في الحرارة فبيتم تعقيم اليوريا أجار في ال
+
+42
+00:03:26,160 --> 00:03:30,320
+filtration مكونات ال media كأي media في عندي
+
+43
+00:03:30,320 --> 00:03:34,040
+normal saline ال normal saline عشان يحافظ على ال
+
+44
+00:03:34,040 --> 00:03:37,900
+osmotic pressure في عندي mono potassium phosphate
+
+45
+00:03:37,900 --> 00:03:43,040
+هذا يعتبر buffer BH هيحافظ ليه على التغير في ال BH
+
+46
+00:03:43,910 --> 00:03:48,730
+البيبتون يعتبر بروتين كمصدر لـ nitrogen source و
+
+47
+00:03:48,730 --> 00:03:53,210
+carbon source لازم لنمو البكتيريا لكن البيبتون
+
+48
+00:03:53,210 --> 00:03:57,510
+الموجود في ميديا اليوريا أجار موجود بكمية قليلة 1
+
+49
+00:03:57,510 --> 00:04:03,010
+gram per liter في عندي اليوريا اليوريا هي ال
+
+50
+00:04:03,010 --> 00:04:06,990
+substrate اللي هيشتغل عليها انزيم اليورياز موجودة
+
+51
+00:04:06,990 --> 00:04:11,980
+بكمية كبيرة 20 gram per literالـ Urea هي الـ
+
+52
+00:04:11,980 --> 00:04:15,160
+Substrate اللي يشغل عليها الانزيم هو بالتالي هتكون
+
+53
+00:04:15,160 --> 00:04:20,740
+الـ CO2 اللي يعتبر ك carbon source لازم لنمو
+
+54
+00:04:20,740 --> 00:04:25,000
+البكتيريا الـ Dextrose اللي هو الجليكوز يعتبر ك
+
+55
+00:04:25,000 --> 00:04:29,200
+energy source كمصدر للطاقة and carbon source برضه
+
+56
+00:04:29,200 --> 00:04:35,360
+موجود بكمية قليلة حتى يعتبر carbon source لازم
+
+57
+00:04:35,360 --> 00:04:39,840
+لنمو البكتيرياأكيد بما أنه ناتج التفاول في عندي
+
+58
+00:04:39,840 --> 00:04:43,960
+alkaline product فهضيف pH indicator عشان أكشف على
+
+59
+00:04:43,960 --> 00:04:49,300
+النواتج ال pH indicator هو ال phenol red ال phenol
+
+60
+00:04:49,300 --> 00:04:53,160
+red مر معنى قبل كده في فحص ال fermentation يبقى
+
+61
+00:04:53,160 --> 00:04:58,840
+عشان نحفظ فحص ال fermentation و فحص اليوريا كان ال
+
+62
+00:04:58,840 --> 00:05:02,560
+pH الموجود ال pH indicator الموجود في ال media هو
+
+63
+00:05:02,560 --> 00:05:08,710
+phenol redالفيه non-red في الacidic بيكون لونه
+
+64
+00:05:08,710 --> 00:05:12,370
+yellow في ال alkaline بيكون لونه red في ال neutral
+
+65
+00:05:12,370 --> 00:05:16,950
+بيكون لونه orange هزرع البكتيريا اللى بدي اكشف
+
+66
+00:05:16,950 --> 00:05:21,390
+عندها انزيم الورياز ولا لأ كيف بدي انا اعمل او كيف
+
+67
+00:05:21,390 --> 00:05:25,330
+بدي ازرع ال slant بعمل stabbing للمنطقة اللى تحت
+
+68
+00:05:25,330 --> 00:05:31,570
+بعدين هطلع زيك زاج طبعا هاقم الفوهة قبل و بعد بعد
+
+69
+00:05:31,570 --> 00:05:37,090
+هعمل incubation لمدة 24 ساعةبعد ال incubation هشوف
+
+70
+00:05:37,090 --> 00:05:42,710
+اللي هي ال result النتيجة بقول لي إذا لاحظت في
+
+71
+00:05:42,710 --> 00:05:46,090
+عندي growth with a change in color of media from
+
+72
+00:05:46,090 --> 00:05:49,530
+pink to red هيكون في عندي نمولة البكتيريا إشهر
+
+73
+00:05:49,530 --> 00:05:53,410
+طبيعي في عندي بيبتون كمصدر لل نيتروجين وال carbon
+
+74
+00:05:53,410 --> 00:05:56,790
+source بكمية قليلة في عندي ديكستراز كمصدر لل
+
+75
+00:05:56,790 --> 00:06:01,180
+carbon source بكمية قليلة خلصالـ Dextrose و
+
+76
+00:06:01,180 --> 00:06:05,200
+البيبتون لأنه موجود في كمية قليلة هتلجأ لمصادر
+
+77
+00:06:05,200 --> 00:06:09,060
+تانية اللي هي ال non carbohydrate carbon source
+
+78
+00:06:09,060 --> 00:06:15,380
+اليوريا لأن عندها أنزيم اليورياز طبعا فهتكسر
+
+79
+00:06:15,380 --> 00:06:21,040
+اليوريا و هحصل على CO2 زائد NH3 عندي carbon source
+
+80
+00:06:21,040 --> 00:06:25,280
+و nitrogen source فبالتالي هتنمو البكتيرياهيتغير
+
+81
+00:06:25,280 --> 00:06:28,340
+لون ال media للون pink to red لون ال media الأصلي
+
+82
+00:06:28,340 --> 00:06:33,080
+بيكون لونه pink لكن لون pink فاتح هيتحول لون ال
+
+83
+00:06:33,080 --> 00:06:38,720
+media للون فوشي للون زهري غامق كتير فبالتالي هذه
+
+84
+00:06:38,720 --> 00:06:41,840
+النتيجة ال positive طيب ليش اتغير لون ال media
+
+85
+00:06:41,840 --> 00:06:46,520
+للون ال pink أو ال red اللون الفوشي الغامق لأنه
+
+86
+00:06:46,520 --> 00:06:50,800
+عندي أنزيم اليورياز كسر اليوريا نتج أمانيا
+
+87
+00:06:50,800 --> 00:06:55,140
+الأمانيا alkaline productالـ alkaline product طبعا
+
+88
+00:06:55,140 --> 00:07:00,340
+عندي أنا pH indicator في نول red في حال كانت في
+
+89
+00:07:00,340 --> 00:07:05,340
+حال اللي هو ال basic هيتغير اللون ال pH indicator
+
+90
+00:07:05,340 --> 00:07:09,960
+لللون الأحمر نتيجة اللون الأحمر اللي هو تكون ال
+
+91
+00:07:09,960 --> 00:07:13,720
+alkaline product الأمانية هيرفعلي ال pH وهيتحول
+
+92
+00:07:13,720 --> 00:07:19,380
+لون ال pH indicator لللون ال redمن الأمثلة على ال
+
+93
+00:07:19,380 --> 00:07:22,080
+positive النتيجة ال positive يورياز positive
+
+94
+00:07:22,080 --> 00:07:26,220
+البروتياز الهيليكو باكتر كليبسيلا نيومانيا انتيرو
+
+95
+00:07:26,220 --> 00:07:30,600
+باكتر ويرسينيا انتيرو كوليتكا بالنسبة للنتيجة ال
+
+96
+00:07:30,600 --> 00:07:36,280
+negative بقولي
+
+97
+00:07:36,280 --> 00:07:40,860
+أن في بكون عندي growth في بكون عندي نمو للبكتيريا
+
+98
+00:07:41,330 --> 00:07:44,670
+إذا بتتذكروا بفحص السيطريات كانت النتيجة النيجاتيف
+
+99
+00:07:44,670 --> 00:07:49,830
+كان عندي اللون الأخضر هو اللون ال media الأصلي قبل
+
+100
+00:07:49,830 --> 00:07:54,510
+ما أنا أزرع uninculated بيكون لونه أخضر و بيكون في
+
+101
+00:07:54,510 --> 00:07:58,430
+عندي نوع growth مش موجود في عندي نمولة البكتيريا
+
+102
+00:07:58,430 --> 00:08:02,550
+لكن في فحص اليوريا النتيجة النيجاتف بيكون في عندي
+
+103
+00:08:02,550 --> 00:08:06,350
+growth في بيكون عندي نمولة البكتيريا طيب ليش
+
+104
+00:08:06,350 --> 00:08:10,470
+هنقولنا ان النتيجة النيجاتف في عندي growthفي فحص
+
+105
+00:08:10,470 --> 00:08:15,370
+السيطرات إذا بتتذكروا ما كان في عندي مصدر آخر للـ
+
+106
+00:08:15,370 --> 00:08:18,710
+carbon غير السيطرات فبالتالي البكتيريا اللي عندها
+
+107
+00:08:18,710 --> 00:08:21,690
+القدرة على استهلاك السيطرات فقط هي اللي هتنمو كان
+
+108
+00:08:21,690 --> 00:08:26,190
+مصدر وحيد للـ carbon اللي هو السيطرات لكن في فحص
+
+109
+00:08:26,190 --> 00:08:29,650
+اليوريا في ميديا اليوريا أجار في عندي بيبتان في
+
+110
+00:08:29,650 --> 00:08:33,130
+عندي دكستراز كلها مصادر لالـ carbon و الـ nitrogen
+
+111
+00:08:33,130 --> 00:08:37,050
+لازمة لنمو البكتيريا فبالتالي حتى لو البكتيريا ما
+
+112
+00:08:37,050 --> 00:08:41,040
+عندها القدرة انهاتكسر اليوريا عندى بيبتون
+
+113
+00:08:41,040 --> 00:08:44,880
+وديكستراز موجود بكمية قليلة هتقدر انها تنمو
+
+114
+00:08:44,880 --> 00:08:49,220
+البكتيريا هذا بالنسبة ليش انه النتيجة ال negative
+
+115
+00:08:49,220 --> 00:08:52,900
+فيه بيكون فيه إجراث with not change in color of
+
+116
+00:08:52,900 --> 00:08:57,580
+media بيكون لون ال media قولنا pink لون زهري فاتح
+
+117
+00:08:57,580 --> 00:09:02,300
+او بيقول ممكن يتحول لون ال media لللون ال yellow
+
+118
+00:09:02,300 --> 00:09:06,060
+ليش بتحول اللون لللون ال yellow لأنه بيقولنا ممكن
+
+119
+00:09:06,060 --> 00:09:11,530
+البكتيرياتعمل fermentation للـ Dextrose يتكون أسد
+
+120
+00:09:11,530 --> 00:09:16,930
+ويتحول لون الـ pH لللون الأصفر يبقى ممكن يكون عنده
+
+121
+00:09:16,930 --> 00:09:20,350
+الـ negative لونه ياله نتيجة اللي هو ال
+
+122
+00:09:20,350 --> 00:09:23,810
+fermentation للـ Dextrose الموجود في ال media
+
+123
+00:09:24,200 --> 00:09:28,520
+هيتكون acidic product فبالتالي هيقلل ال BH ويتحول
+
+124
+00:09:28,520 --> 00:09:33,480
+ال BH Indicator لللون الأصفر من الأمثلة على ال
+
+125
+00:09:33,480 --> 00:09:38,980
+Ureas Negative السلمونيلا والشيجيلا يعتبروا Ureas
+
+126
+00:09:38,980 --> 00:09:43,200
+Negative بالنسبة
+
+127
+00:09:43,200 --> 00:09:50,810
+لهذا المكتوب بعمل incubation لمدة 24 ساعةtwo four
+
+128
+00:09:50,810 --> 00:09:55,490
+days بقول لي مابقدر أكتب النتيجة negative إلا بعد
+
+129
+00:09:55,490 --> 00:10:00,490
+أربع أيام من ال incubation يبقى بطلع ال tube بعد
+
+130
+00:10:00,490 --> 00:10:05,590
+24 ساعة إذا طلعت negative برجحها على ال incubator
+
+131
+00:10:05,590 --> 00:10:10,430
+بعد أربع أيام إذا ضلت negative خلاص بسجل النتيجة
+
+132
+00:10:10,430 --> 00:10:14,970
+negative لكن مابسجل النتيجة negative قبل أربع أيام
+
+133
+00:10:14,970 --> 00:10:19,600
+ليش؟ why؟ هذا السؤال مهم جداواللي في عندي بكتيريا
+
+134
+00:10:19,600 --> 00:10:24,800
+delayed urease positive بتاخد وقت أطول اكتر من 48
+
+135
+00:10:24,800 --> 00:10:30,560
+ساعة عشان تكسر ال urea وتنتج ال ammonia وترفع ال
+
+136
+00:10:30,560 --> 00:10:36,180
+pH ويتحول لون الوسط للون ال pink عشان هيك بخليها ل
+
+137
+00:10:36,180 --> 00:10:41,700
+4 ايام في المقابل في عندي rapid urease positive
+
+138
+00:10:41,700 --> 00:10:45,980
+هذه البكتيريا خلال 6 ساعات يعني قبل ال 24 ساعة
+
+139
+00:10:45,980 --> 00:10:50,820
+بتحول لونالميديا لللون الفوشى زى البروتياس
+
+140
+00:10:50,820 --> 00:10:54,800
+والهيليكوباكتر هدول يعتبروا rapid urease positive
+
+141
+00:10:54,800 --> 00:10:58,700
+حتى أنا بلاحظ خلال الست ساعات الأولى انه اتحول لون
+
+142
+00:10:58,700 --> 00:11:05,060
+ال slant لللون الفوشى لكن ال بالنسبة للبط اللى
+
+143
+00:11:05,060 --> 00:11:10,320
+تحتها ده بيكون لونه أصفر بعد 24 ساعة كل تيوب بيصير
+
+144
+00:11:10,320 --> 00:11:15,310
+لونها فوشىهذه بالنسبة ليش ان انا مكتوبة هنا بخليها
+
+145
+00:11:15,310 --> 00:11:20,270
+ل 4 أيام لان قلنا في عندي delayed urease positive
+
+146
+00:11:20,270 --> 00:11:24,670
+في نقطة تالتة مهمة بالنسبة لفحص ال urease test
+
+147
+00:11:24,670 --> 00:11:30,430
+بقول لي في عندي في ال media بيبتون قلنا ان بعض
+
+148
+00:11:30,430 --> 00:11:34,710
+أنواع البكتيريا بتعمل deamination للبيبتون مش يعني
+
+149
+00:11:34,710 --> 00:11:39,890
+deamination للبيبتون يعني هتنزع ال ammonia من
+
+150
+00:11:39,890 --> 00:11:44,120
+البيبتون من البروتينوهيرفع بما انه انتزعت ال
+
+151
+00:11:44,120 --> 00:11:47,020
+ammonia طلعت ال ammonia هيرتفع ال pH ال ammonia
+
+152
+00:11:47,020 --> 00:11:51,980
+اللي خلانه product هيتحول اللون رفعت ال pH صار
+
+153
+00:11:51,980 --> 00:11:56,780
+basic هيتحول اللون ال media لللون الأحمر او ال
+
+154
+00:11:56,780 --> 00:12:00,780
+deep pink طب السؤال هان كيف بدي افرق ان اللون
+
+155
+00:12:00,780 --> 00:12:04,320
+الأحمر كان نتيجة تكسير اليوريا ولا ال بيبطان
+
+156
+00:12:04,320 --> 00:12:11,490
+بقوللي انه التفسير لهذا الموضوعإن كمية البيبتون
+
+157
+00:12:11,490 --> 00:12:15,650
+الموجودة في الميديا قلنا 1 gram per liter لكن
+
+158
+00:12:15,650 --> 00:12:20,590
+اليوريا كانت 20 gram per liter يبقى كمية البيبتون
+
+159
+00:12:20,590 --> 00:12:25,130
+بالنسبة لليوريا كثير قليلة في عندي أنا كمان buffer
+
+160
+00:12:25,130 --> 00:12:30,030
+في الميديا mono potassium phosphate هذا ال buffer
+
+161
+00:12:30,030 --> 00:12:33,910
+هيحافظلي على التغير في البيئة لو البكتيريا عملت
+
+162
+00:12:33,910 --> 00:12:37,050
+deamination للبيبتون اللي كميته قليلة بالنسبة
+
+163
+00:12:37,050 --> 00:12:41,780
+لليورياالـ buffer هيقدر يعادل هذا التغير في الـ pH
+
+164
+00:12:41,780 --> 00:12:47,540
+لكن لو كانت نتيجة تكسير اليوريا اليوريا الموجودة
+
+165
+00:12:47,540 --> 00:12:52,800
+في ال media كتيرة فبالتالي ما بيقدر ال buffer يعمل
+
+166
+00:12:52,800 --> 00:12:57,820
+معادلة لإلها فبكون فقط اللون الأحمر تكون من تكسير
+
+167
+00:12:57,820 --> 00:13:04,980
+اليوريا هذا بالنسبة لـأهم الأشياء اللي في فحص
+
+168
+00:13:04,980 --> 00:13:09,640
+اليورياز بشكل ملخص ممكن احنا قولنا اني انا ازرع
+
+169
+00:13:09,640 --> 00:13:14,900
+بسلانت او بروث او اجار ابلات نيجي نمور على ال
+
+170
+00:13:14,900 --> 00:13:19,920
+slides يورياز
+
+171
+00:13:19,920 --> 00:13:23,040
+test some bacteria are able to produce an enzyme
+
+172
+00:13:23,040 --> 00:13:25,700
+called urease بقولي بعض أنواع البكتيريا عندها
+
+173
+00:13:25,700 --> 00:13:29,510
+القدرة انها تفرز انزيم اسمه اليوريازالذي يهاجم
+
+174
+00:13:29,510 --> 00:13:33,270
+نيتروجين والكربون في مجموعات عميقية مثل اليوريا
+
+175
+00:13:33,270 --> 00:13:34,690
+يعمل تجارب تجارب تجارب تجارب تجارب تجارب تجارب
+
+176
+00:13:34,690 --> 00:13:35,730
+تجارب تجارب تجارب تجارب تجارب تجارب تجارب تجارب
+
+177
+00:13:35,730 --> 00:13:36,270
+تجارب تجارب تجارب تجارب تجارب تجارب تجارب تجارب
+
+178
+00:13:36,270 --> 00:13:36,670
+تجارب تجارب تجارب تجارب تجارب تجارب تجارب تجارب
+
+179
+00:13:36,670 --> 00:13:36,670
+تجارب تجارب تجارب تجارب تجارب تجارب تجارب تجارب
+
+180
+00:13:36,670 --> 00:13:56,310
+تجارب تجارب تجارب تجارب تجارب تجارب تجارب تجارب ت
+
+181
+00:13:56,630 --> 00:13:59,050
+helping to differentiate salmonella and shigella
+
+182
+00:13:59,050 --> 00:14:03,130
+species which are urease negative from the urease
+
+183
+00:14:03,130 --> 00:14:05,610
+positive non-battaging protease and some
+
+184
+00:14:05,610 --> 00:14:08,790
+citrobacter species and some homophilous species
+
+185
+00:14:08,790 --> 00:14:15,070
+are urease positive هذا الفقرة كلها ال significant
+
+186
+00:14:15,070 --> 00:14:20,970
+لفحص ال urease شو ال significant لفحص ال urease
+
+187
+00:14:20,970 --> 00:14:26,290
+فحص ال urease بقولليانه بستخدمه عشان افرق ان ما ال
+
+188
+00:14:26,290 --> 00:14:28,910
+lactose fermenter قولنا عائلة ال
+
+189
+00:14:28,910 --> 00:14:32,710
+enterobacteriaceae اللى هى تعتبر إجرام نيجاتيف
+
+190
+00:14:32,710 --> 00:14:40,530
+بصلية اكسيداز نيجاتيف بنميها على المقنكى اصده هان
+
+191
+00:14:40,530 --> 00:14:46,260
+ال differential media اللى هى المقنكىالمقنكي تعتبر
+
+192
+00:14:46,260 --> 00:14:49,480
+differential media هو selective media selective
+
+193
+00:14:49,480 --> 00:14:52,520
+media لأنه بتنمي فقط لجرام negative بكتيريا
+
+194
+00:14:52,520 --> 00:14:56,020
+مابتنمي لجرام positive بكتيريا differential media
+
+195
+00:14:56,020 --> 00:15:01,840
+لأنه بتميز اللكتاز عن ال non-lactose fermenter فلو
+
+196
+00:15:01,840 --> 00:15:05,880
+نمت البكتيريا اللكتاز fermenter بتعطيها اللون ال
+
+197
+00:15:05,880 --> 00:15:10,000
+pink لكن لو نمت البكتيريا ال non-lactose fermenter
+
+198
+00:15:10,000 --> 00:15:15,900
+بتعطيها اللون ال colorless هلأ بقولليفي هذه الفقرة
+
+199
+00:15:15,900 --> 00:15:22,360
+لو أنا وصلتني عينة شتول بروح
+
+200
+00:15:22,360 --> 00:15:25,740
+بزراها على الـ differential media المكونكي احنا
+
+201
+00:15:25,740 --> 00:15:31,160
+قلنا الـ microbiologist بختار نوع ال media حسب نوع
+
+202
+00:15:31,160 --> 00:15:36,980
+العينة من الميديات اللي انا بزراها في حال وصلتني
+
+203
+00:15:36,980 --> 00:15:44,460
+عينة شتول مكونكي بزرع عينة الشتول على مكونكيبعد ما
+
+204
+00:15:44,460 --> 00:15:48,260
+انا زراعة عينة الستول على مقنقي طلعت النتيجة non
+
+205
+00:15:48,260 --> 00:15:52,100
+-lactose fermenter كان لون ال كولوني لونها
+
+206
+00:15:52,100 --> 00:15:56,240
+colorless بقوللي في عندي تلت احتمالات ال non
+
+207
+00:15:56,240 --> 00:15:59,640
+-lactose fermenter بتتكون من مجموعة من البكتيريا
+
+208
+00:15:59,640 --> 00:16:03,680
+لكن في الستول في بكون عندي تلت احتمالات اما انها
+
+209
+00:16:03,680 --> 00:16:08,980
+تكون Salmonella, Shigellaالسلمونيلا والشيجيلا
+
+210
+00:16:08,980 --> 00:16:13,060
+يعتبروا pathogen in stool البروتياس يعتبر non
+
+211
+00:16:13,060 --> 00:16:16,680
+pathogen كيف بدي أفرق بين هدولة التلاتة بقولي
+
+212
+00:16:16,680 --> 00:16:22,500
+بتعملي فحص الورياز الورياز هيميز ال non lactose
+
+213
+00:16:22,500 --> 00:16:27,360
+fermenter في ال stool specimen على طبق أو على
+
+214
+00:16:27,360 --> 00:16:32,800
+ميديا الماكنكي لو طلعت ورياز negative يبقى هي
+
+215
+00:16:32,800 --> 00:16:36,920
+سلمونيلا وشيجيلالكن هي مكتوب salmonella and
+
+216
+00:16:36,920 --> 00:16:41,360
+shigella species which are urease negative لكن ال
+
+217
+00:16:41,360 --> 00:16:45,440
+urease positive اللى تعتبر non pathogen في ال
+
+218
+00:16:45,440 --> 00:16:49,080
+stool specimen اللى هي تعتبر ال protease ال
+
+219
+00:16:49,080 --> 00:16:52,800
+protease تعتبر urease positive يبقى بعمل فحص ال
+
+220
+00:16:52,800 --> 00:16:57,140
+urease لو طلعت positive فهي protease تعتبر non
+
+221
+00:16:57,140 --> 00:17:01,320
+pathogen في ال stool specimen لكن لو طلعت negative
+
+222
+00:17:01,320 --> 00:17:06,350
+يبقى هي salmonella او shigella هنكتب اللى فيهورياز
+
+223
+00:17:06,350 --> 00:17:10,110
+بوزيتف غير البروتياس some citrobacter species and
+
+224
+00:17:10,110 --> 00:17:14,770
+some hemophilus species يعتبروا برضه يورياز بوزيتف
+
+225
+00:17:14,770 --> 00:17:18,810
+بروتياس ميرابيلس is a major cause of human urinary
+
+226
+00:17:18,810 --> 00:17:22,910
+tract infection هان احنا قلنا انه البروتياس تعتبر
+
+227
+00:17:22,910 --> 00:17:27,870
+non pathogen in stool لكن هان بقوللي هي pathogen
+
+228
+00:17:27,870 --> 00:17:31,430
+في ال urine يبقى البروتياس ميرابيلس هي ال major
+
+229
+00:17:31,430 --> 00:17:36,390
+cause of human urinary tract infectionعشان هيك لو
+
+230
+00:17:36,390 --> 00:17:42,670
+مريض بيه الـ UTI كان وكان سبب الـ UTI بروتياس
+
+231
+00:17:42,670 --> 00:17:49,090
+بنلاحظ أنه رائحة الـ urine بتكون في رائحة اللي هي
+
+232
+00:17:49,090 --> 00:17:53,890
+رائحة الأمانيا نتيجة تكسير الـ urea بواسطة أنزيم
+
+233
+00:17:53,890 --> 00:17:57,590
+الـ ureas لأن البروتياس ureas positive فهيكسر الـ
+
+234
+00:17:57,590 --> 00:18:02,230
+urea هيطلع أمانيا الرائحة اللي هي رائحة الأمانيا
+
+235
+00:18:02,630 --> 00:18:07,490
+هذا بالنسبة لـ Significant لفحص اليورياز عشان يفرق
+
+236
+00:18:07,490 --> 00:18:10,150
+ال salmonella و الشجلة اللي يعتبر يورياز negative
+
+237
+00:18:10,680 --> 00:18:15,640
+باثوجين في الستول عن اليورياز positive اللى هى ال
+
+238
+00:18:15,640 --> 00:18:19,040
+non pathogen اللى هى البروتياز طب كيف دي افرق
+
+239
+00:18:19,040 --> 00:18:23,180
+السلمونيلا و الشيجيلا عن بعض لو طلعت يورياز
+
+240
+00:18:23,180 --> 00:18:27,620
+negative بقول في فحص ال motility و فحص ال H2S
+
+241
+00:18:27,620 --> 00:18:31,760
+السلمونيلا positive للفحسين motile and H2S
+
+242
+00:18:31,760 --> 00:18:36,420
+positive الشيجيلا non motile and H2S negative
+
+243
+00:18:36,950 --> 00:18:41,850
+البروتياس نفس السلمونيلا موتايل زائد H2S positive
+
+244
+00:18:41,850 --> 00:18:45,890
+طبعا بفرق بينهم بفحص اللي هو اليورياز البروتياس
+
+245
+00:18:45,890 --> 00:18:50,690
+positive والسلمونيلا negative لليورياز هذا بالنسبة
+
+246
+00:18:50,690 --> 00:18:58,750
+ل ال significant للفحص اليورياز
+
+247
+00:18:58,750 --> 00:19:02,070
+producing by some enterobacteriasemia اليورياز
+
+248
+00:19:02,070 --> 00:19:04,150
+positive قولنا البروتياس لكلبسلال
+
+249
+00:19:04,150 --> 00:19:08,260
+enterobacterialiersinia enterocoliticaالهيليكوبكتر
+
+250
+00:19:08,260 --> 00:19:12,520
+بيولوري هو أكسداز بوزيتف قلنا الهيليكوبكتر زي ال
+
+251
+00:19:12,520 --> 00:19:19,380
+.. زي ال vibrio cholera زي ال aeromonas هدال كانوا
+
+252
+00:19:19,380 --> 00:19:27,000
+أكسداز بوزيتف لكن كانت ال Sodomonas أكسداز زيهم
+
+253
+00:19:27,000 --> 00:19:31,960
+Sodomonas أكسداز بوزيتف الهيليكوبكتر ال aeromonas
+
+254
+00:19:31,960 --> 00:19:36,640
+ال vibrio لكن الإختلاف بينها وبين Sodomonasإنه الـ
+
+255
+00:19:36,640 --> 00:19:40,500
+Helicobacter بقول لي هي Gelicose Fermenter لكن
+
+256
+00:19:40,500 --> 00:19:45,520
+الصدومونس كانت Non-Gelicose Fermenter الـ
+
+257
+00:19:45,520 --> 00:19:49,220
+Helicobacter قلنا إنه هي Urease Positive بكتيرية
+
+258
+00:19:49,220 --> 00:19:53,100
+تعتبر Rapid Urease Positive خلال ست ساعات بتتحول
+
+259
+00:19:53,100 --> 00:19:57,180
+اللون للون بنكية و البروتياس الـ Helicobacter و
+
+260
+00:19:57,180 --> 00:20:00,400
+البروتياس يعتبرهم Rapid Urease Positive
+
+261
+00:20:05,110 --> 00:20:07,530
+الـ Principle عشان اني انا افرق اليوريا is
+
+262
+00:20:07,530 --> 00:20:10,150
+positive و اليوريا is negative بستخدم اللي هي
+
+263
+00:20:10,150 --> 00:20:14,190
+اليوريا agar قولنا بتحتوي على يوريا بنسبة 20 جرام
+
+264
+00:20:14,190 --> 00:20:20,750
+per liter per liter جيلاتين بيبتون هدول مصادر
+
+265
+00:20:20,750 --> 00:20:24,750
+يعتبر ال protein مصادر ال nitrogen و ال carbon
+
+266
+00:20:24,750 --> 00:20:28,250
+source موجود بكمية قليلة صوديوم الكلورايت قولنا
+
+267
+00:20:28,250 --> 00:20:31,770
+عشان يحافظ على ال ozone pressure ديكستروز موجود
+
+268
+00:20:31,770 --> 00:20:36,460
+بكمية قليلة قولنا كمصدر للكربونمصدر للطاقة في non
+
+269
+00:20:36,460 --> 00:20:40,620
+-red هو الـ pH Indicator مونوبوتاسيومفوسفات قلنا
+
+270
+00:20:40,620 --> 00:20:44,100
+اللي هو ال buffer اللي هيحفظلي على التغير في الـ
+
+271
+00:20:44,100 --> 00:20:47,700
+pH some bacteria can utilize urea as non
+
+272
+00:20:47,700 --> 00:20:50,480
+-carbohydrate carbon source قلنا الـ urea تعتبر
+
+273
+00:20:50,480 --> 00:20:53,940
+non-carbohydrate carbon source باستخدام أنزيم الـ
+
+274
+00:20:53,940 --> 00:20:59,420
+urease هي أنزيم الـ urease وهي الـ urea هي الرابط
+
+275
+00:20:59,420 --> 00:21:03,410
+ما بين الكربون والنيتروجينمابين الكربون
+
+276
+00:21:03,410 --> 00:21:07,190
+والنيتروجين هتيجي اليورياز ويكسر هاي الرابطة
+
+277
+00:21:07,190 --> 00:21:12,630
+ويتكون عندي أمونيا زائد CO2 يورياز activity the
+
+278
+00:21:12,630 --> 00:21:15,890
+urea is detected by growing bacteria in media
+
+279
+00:21:15,890 --> 00:21:20,430
+containing urea يوريا هتكون كsubstrate وبستخدم pH
+
+280
+00:21:20,430 --> 00:21:25,130
+indicator اللي هو ال phenol red لو اليوريا اتكسرت
+
+281
+00:21:25,130 --> 00:21:29,690
+بواسطة انزيم اليورياز الامونيا هتكون ناتج من نواتج
+
+282
+00:21:29,690 --> 00:21:36,530
+عمليةالتكسير فبالتالي هيعملي الوسط alkaline هيرفع
+
+283
+00:21:36,530 --> 00:21:40,810
+ال pH فبالتالي ال indicator هيتحول لونه للون
+
+284
+00:21:40,810 --> 00:21:47,150
+الأحمر to deep pink او انه هيتحول للون زي ال
+
+285
+00:21:47,150 --> 00:21:51,750
+violet هذا هو ال positive لفحص اليوريا Dextrose
+
+286
+00:21:51,750 --> 00:21:54,790
+قلنا موجود بكمية قليلة في ال media فبالتالي
+
+287
+00:21:54,790 --> 00:22:01,130
+البكتيريا هتروح تلجأ لمصدر تاني ل carbon sourceأو
+
+288
+00:22:01,130 --> 00:22:05,290
+انها تتوقف على النمو في حال ما كان في عندها انزيم
+
+289
+00:22:05,290 --> 00:22:10,870
+اليورياز اكيد هتتوقف على النمو لأن البيبتون و
+
+290
+00:22:10,870 --> 00:22:14,630
+الديكستراز اللي يعتبروا كمصدر الكاربون اللازم لنمو
+
+291
+00:22:14,630 --> 00:22:19,410
+البكتيريا خلص موجود بكمية قليلة هتلجأ لا اليورياز
+
+292
+00:22:19,410 --> 00:22:23,470
+في حال ما كان عندها يورياز اكيد هتتوقف على النمو
+
+293
+00:22:23,470 --> 00:22:27,790
+في اي حال عندها يورياز البكتيريا كانت يورياز
+
+294
+00:22:27,790 --> 00:22:31,930
+positive اكيد هتلجأ لامصدر اليوريا عشان تحصل على
+
+295
+00:22:31,930 --> 00:22:36,790
+carbon source نلاحظ
+
+296
+00:22:36,790 --> 00:22:40,610
+في الصورة أنه في non-red في البيئة في الاسدك بيكون
+
+297
+00:22:40,610 --> 00:22:45,270
+لونه yellow لكن في ال basic بيكون لونه فوشي هيك
+
+298
+00:22:45,270 --> 00:22:49,090
+بيكون النتيجة ال positive هذا اللون الفوشي في حال
+
+299
+00:22:49,090 --> 00:22:50,630
+كان عندي يورياز positive
+
+300
+00:22:53,640 --> 00:22:58,600
+الطريقة أني أنا بزرع ال slant في ال media اليوريا
+
+301
+00:22:58,600 --> 00:23:02,400
+بال test organism بال organism اللي بدي أكشف عنه
+
+302
+00:23:02,400 --> 00:23:07,600
+هعمل incubation لمدة 24 ساعة ل 4 days و عرفنا ليش
+
+303
+00:23:07,600 --> 00:23:13,040
+أنا بدي أخلي اليوتيوب لمدة 4 أياملأن في عندي delay
+
+304
+00:23:13,040 --> 00:23:19,560
+urease positive بكتيريا النتيجة بشوفها بعد 48 ساعة
+
+305
+00:23:19,560 --> 00:23:22,840
+هي some bacteria have a delay urease reaction that
+
+306
+00:23:22,840 --> 00:23:27,180
+may require an incubation period longer than أكتر
+
+307
+00:23:27,180 --> 00:23:31,760
+من 48 ساعة النتيجة ال positive بشوف لون bright
+
+308
+00:23:31,760 --> 00:23:35,320
+pink color هيتطور فيه الإسلان بعدين ممكن أنه يمتد
+
+309
+00:23:35,320 --> 00:23:39,070
+في ال media خلال أول تلت ساعات بشوفه بالإسلانفلما
+
+310
+00:23:39,070 --> 00:23:43,150
+اتكمل ال 24 ساعة بشوفه ممتد في كل ال media في ال
+
+311
+00:23:43,150 --> 00:23:46,730
+negative لا تغير في ال original color of media مش
+
+312
+00:23:46,730 --> 00:23:49,510
+هلاحظ اي تغير في لون ال media او قولنا هيتحول
+
+313
+00:23:49,510 --> 00:23:56,550
+اللون للون الأصفر هذه نتيجة ال positive و ال
+
+314
+00:23:56,550 --> 00:23:59,370
+negative هان احنا قولنا ممكن تكون breath ممكن تكون
+
+315
+00:23:59,370 --> 00:24:02,510
+slant هذه breath النتيجة ال negative ما تغير لون
+
+316
+00:24:02,510 --> 00:24:05,310
+ال media النتيجة ال positive كان في عندي اللون
+
+317
+00:24:05,310 --> 00:24:09,240
+فوشيhigh slant النتيجة ال positive اللون فوشي في
+
+318
+00:24:09,240 --> 00:24:13,560
+كل تيوب لكن ال negative ما تغير لون ال media ال
+
+319
+00:24:13,560 --> 00:24:16,860
+rabbit positive قلنا خلال أول ست ساعات بلاحظ أن
+
+320
+00:24:16,860 --> 00:24:21,840
+اللون الفوشي كان في ال slant لكن لو أنا خلاتها أو
+
+321
+00:24:21,840 --> 00:24:26,760
+كملت ل 24 ساعة هلاحظ أنه هيمتد اللون الفوشي في كل
+
+322
+00:24:26,760 --> 00:24:30,920
+تيوب high negative هي ما تغير لون ال media دلوا
+
+323
+00:24:30,920 --> 00:24:35,300
+اللون اللي ما أخد no change on color of the media
+
+324
+00:24:35,730 --> 00:24:39,390
+هذا بالنسبة لفحص اليورياز
+
+325
+00:24:47,090 --> 00:24:51,570
+هنيجي هلأ للفحص التاني اللي هو ال Indole Test ال
+
+326
+00:24:51,570 --> 00:24:55,430
+Indole Test هيكشف ليه على أنزيم لتريبتوفاناز
+
+327
+00:24:55,430 --> 00:25:00,550
+لتريبتوفاناز يعتبر اندو انزايم من اسمه هيشتغل على
+
+328
+00:25:00,550 --> 00:25:05,050
+لتريبتوفان as substrate لتريبتوفان يعتبر amino
+
+329
+00:25:05,050 --> 00:25:09,790
+acid ال amino acid لتريبتوفاناز هيكسر هذا ال amino
+
+330
+00:25:09,790 --> 00:25:13,950
+acid عشان أحصل على carbon source و nitrogen source
+
+331
+00:25:13,950 --> 00:25:20,150
+لازم لنمو البكتيرياالتريبتوفان من مكوناته اندول و
+
+332
+00:25:20,150 --> 00:25:24,010
+بيروفات و أمونيا هدولة تلاتة لكن بيكونوا متصلين
+
+333
+00:25:24,010 --> 00:25:28,990
+ببعض مع بعض مرتبطين في حال البكتيريا أفرت ذات
+
+334
+00:25:28,990 --> 00:25:33,230
+إنزيم للتريبتوفانات هتكسر التريبتوفان اللي هو ال
+
+335
+00:25:33,230 --> 00:25:38,810
+substrate هتعطيني اندول زائد بيروفات زائد أمونيا
+
+336
+00:25:39,840 --> 00:25:43,400
+هدولة التلاتة هتعطيهم بشكل منفصل كانوا هم مع بعض
+
+337
+00:25:43,400 --> 00:25:49,040
+مرتبطين ببعض ب .. لتريبتوفان باسم لتريبتوفان
+
+338
+00:25:49,040 --> 00:25:51,640
+لتريبتوفان عبارة عن اندول و بيروفيت و أمونيا
+
+339
+00:25:51,640 --> 00:25:55,660
+مرتبطين ببعض إجا أنزيم لتريبتوفانياز كسر
+
+340
+00:25:55,660 --> 00:25:59,880
+لتريبتوفان و أعطاني اندول زائد بيروفيت زائد أمونيا
+
+341
+00:25:59,880 --> 00:26:03,780
+طبعا الأمونيا تستخدم ك نيتروجين سورس البيروفيت
+
+342
+00:26:03,780 --> 00:26:09,150
+تستخدم لمصدد التقوي ال carbon sourceهذا بالنسبة
+
+343
+00:26:09,150 --> 00:26:13,750
+للفحص ال principle كيف انا بدي اشتغل هذا الفحص
+
+344
+00:26:13,750 --> 00:26:17,790
+بقوللي بجيب media بتحتوي على ال substrate ال
+
+345
+00:26:17,790 --> 00:26:22,410
+triptofan ممكن استخدم media اسمها ال triptone
+
+346
+00:26:22,410 --> 00:26:26,370
+broth بتكون ال broth بتحتوي على ال triptofan ال
+
+347
+00:26:26,370 --> 00:26:31,850
+substrate او ممكن استخدم media اسمها sim ال sim
+
+348
+00:26:31,850 --> 00:26:38,150
+بتكون هاي ال media slant مش ال broth ال simأو
+
+349
+00:26:38,150 --> 00:26:44,670
+sorry بتكون stab مش slant انعدل هذه الكلمة ال S I
+
+350
+00:26:44,670 --> 00:26:48,150
+M بتكون stab زي اللي استخدمناها في فحص ال motility
+
+351
+00:26:48,150 --> 00:26:54,610
+ال S بترمز أو بتكشف على فحص ال H2S ال I بتكشف على
+
+352
+00:26:54,610 --> 00:26:59,670
+فحص ال indol و ال M بتكشف على فحص ال motility هذه
+
+353
+00:26:59,670 --> 00:27:03,190
+ال media تعتبر single media multiple test هنتعرف
+
+354
+00:27:03,190 --> 00:27:07,290
+في المحاضرة الجايإن أنا ميديا واحدة ممكن تكشفلي
+
+355
+00:27:07,290 --> 00:27:10,830
+على أكتر من فحص كيميائي فهتكشف على الـ edge to
+
+356
+00:27:10,830 --> 00:27:13,790
+edge وهتكشف على ال motility وهتكشف على ال indoor
+
+357
+00:27:13,790 --> 00:27:18,450
+فممكن أستخدم هذه الميديا أو ممكن أستخدم ميديا
+
+358
+00:27:18,450 --> 00:27:22,370
+لتريبتون إبروث اللي فقط هتكشفلي على ال indoor لكن
+
+359
+00:27:22,370 --> 00:27:26,330
+هذه الميديا هتكشفلي على تلت فحوصات من ضمنهم ال
+
+360
+00:27:26,330 --> 00:27:31,510
+indoor لو استخدمت لتريبتون إبروث هاخد البكتيريا
+
+361
+00:27:31,510 --> 00:27:36,290
+اللي بدي أكشف هل انتالنزيم للتريبتوفاناس وهعمل mix
+
+362
+00:27:36,290 --> 00:27:42,590
+في البروث لو كانت SIM هي أستاذ فهعمل stabbing طعنة
+
+363
+00:27:42,590 --> 00:27:48,470
+في المنتصف و هشوف النتيجة بعد 24 ساعة هعمل
+
+364
+00:27:48,470 --> 00:27:53,910
+incubation لمدة 24 ساعةبقولنا انه عشان اكشف عن
+
+365
+00:27:53,910 --> 00:27:59,070
+التفاعل انه صار ولا لأ بدي اضيف reagent هذا ال
+
+366
+00:27:59,070 --> 00:28:03,210
+reagent هيرتبط مع احد لو هتنجح التفاعل هيرتبط مع
+
+367
+00:28:03,210 --> 00:28:08,570
+واحد من هدول هيعطيني لون هيك بعرف ان التفاعل صار
+
+368
+00:28:08,570 --> 00:28:12,970
+صار ولا لأ بقوللي في فحص ال indulge test بعد ال
+
+369
+00:28:12,970 --> 00:28:18,290
+incubation بتضيفي covax reagent ال covax reagent
+
+370
+00:28:18,290 --> 00:28:23,200
+بقوللييرتبط او من مكونات الـ COVAX REAGENT بالأول
+
+371
+00:28:23,200 --> 00:28:29,500
+خلّيني نحكي ISOAMILE ALCOHOL HCL وفيه مادة اللي هي
+
+372
+00:28:29,500 --> 00:28:36,700
+ال BMAB هدولة التلت مكونات من مكونات الـ COVAX
+
+373
+00:28:36,700 --> 00:28:40,800
+REAGENT هضيف الـ COVAX REAGENT بعد 24 ساعة
+
+374
+00:28:40,800 --> 00:28:45,080
+incubation هعمل شكلة لتيوب و هقرأ النتيجة
+
+375
+00:28:45,080 --> 00:28:50,050
+immediatelyبقول لي لو اتكون حلقة لونها cherry red
+
+376
+00:28:50,050 --> 00:28:55,150
+ring زي لون الكرز يبقى هي positive للفحص
+
+377
+00:28:55,150 --> 00:28:58,970
+الاندواليندها انزيم لـ tryptophanase مثال عليها
+
+378
+00:28:58,970 --> 00:29:05,150
+الـ E.coli لو صار ما لو ما صار عندي اي تغيير ما
+
+379
+00:29:05,150 --> 00:29:09,470
+اتكون حلقة لونها زي لون الكرز يبقى هي negative زي
+
+380
+00:29:09,470 --> 00:29:13,510
+ال E.clipsilla و ال Enterobacter طيب كيف صار
+
+381
+00:29:13,510 --> 00:29:18,030
+التفاعل او كيف اتكون الحلقة الحمراءقلنا إن الـ
+
+382
+00:29:18,030 --> 00:29:22,050
+Covax reagent اللي أنا أضفته برتبط مع أحد نواتج
+
+383
+00:29:22,050 --> 00:29:26,210
+التفاعل و اللي ال Covax reagent برتبط مع ال Indol
+
+384
+00:29:26,210 --> 00:29:34,890
+مين بالتحديد اللي بيرتبط مع ال Indol من مكونات ال
+
+385
+00:29:34,890 --> 00:29:38,990
+Covax reagentمن مكونات الـ Govex Reagent اللى
+
+386
+00:29:38,990 --> 00:29:43,810
+بيرتبط مع الاندالي اللى هو ال بي ام ابي هدى
+
+387
+00:29:43,810 --> 00:29:49,090
+هيرتبطوا تنين هدول مع بعض تمام ويكونولى Complex
+
+388
+00:29:49,090 --> 00:29:53,670
+هذا ال Complex هيرتبط مع بعضترسّب في القاع تمام في
+
+389
+00:29:53,670 --> 00:29:59,090
+قاع اليوتيوب هيترسّب هييجي ال HCL هيرفع ال complex
+
+390
+00:29:59,090 --> 00:30:03,070
+و يتكون حلقة في أعلى اليوتيوب يبقى كذا فالتفاعل
+
+391
+00:30:03,070 --> 00:30:06,970
+بيصير ال triptofan يزكسر ال triptofan الموجود في
+
+392
+00:30:06,970 --> 00:30:10,990
+ال media بعد ال incubation هضيف ال covax reagent
+
+393
+00:30:10,990 --> 00:30:17,490
+ال covax reagent أحد مكوناته ال BMABهذه هترتبط مع
+
+394
+00:30:17,490 --> 00:30:22,170
+نواتج التفاعل اللى هو بالتحديد الاندول هيكوّن ليه
+
+395
+00:30:22,170 --> 00:30:26,990
+Complex هيترسّب في قاع ال tube هيجي ال HCl اللى
+
+396
+00:30:26,990 --> 00:30:31,370
+يعتبر مكوّن من مكونات ال covax reagent هيرفع ال
+
+397
+00:30:31,370 --> 00:30:37,430
+complex ويكوّن اللى هو الحلقة الحمراء بقولي ال HCl
+
+398
+00:30:37,430 --> 00:30:41,290
+لو كان لو أنا استخدمت تربتون البروثممكن إن أنا
+
+399
+00:30:41,290 --> 00:30:46,110
+أستغني عنه من ما أضيفه على الـ Covax Reagent لكن
+
+400
+00:30:46,110 --> 00:30:51,810
+في حال كانت ال media SIM اللي هي Stabbing بتكون
+
+401
+00:30:51,810 --> 00:30:55,910
+semi solid فبالتالي صعب إن أنا أستغني عنه لأنه ما
+
+402
+00:30:55,910 --> 00:31:03,290
+بيقدر يطلع لفوق بحتاج لـ SCL هو اللي يطلعه لفوقهذا
+
+403
+00:31:03,290 --> 00:31:08,210
+بالنسبة للفحص هيو الـ N دول قلنا بيرتبط مع الـ
+
+404
+00:31:08,210 --> 00:31:12,670
+PMAB هيتوّن Complex اللي هي الـ Sherry Red هتترسب
+
+405
+00:31:12,670 --> 00:31:17,190
+في القاح هييجي الـ SCL يرفعها بقول لي في Reagent
+
+406
+00:31:17,190 --> 00:31:20,890
+تاني ممكن اني انا في حال ما في عندي Covax Reagent
+
+407
+00:31:20,890 --> 00:31:25,850
+اني انا اضيفه عشان اكشف انه التفاعل صاراسمه
+
+408
+00:31:25,850 --> 00:31:29,850
+Ehrlich-reagent من مكوناته نفس المكونات تبعت
+
+409
+00:31:29,850 --> 00:31:34,130
+الكوفكس-reagent لكن الاختلاف بدل ال Isoamyl
+
+410
+00:31:34,130 --> 00:31:38,870
+Alcohol هستخدم Ethyl Alcohol الفرق بين الكوفكس وال
+
+411
+00:31:38,870 --> 00:31:43,510
+Ehrlich-reagent أنه ال Ehrlich-reagent يعتبر more
+
+412
+00:31:43,510 --> 00:31:48,990
+sensitive يعني لو كمية قليلةموجودة في ال media الـ
+
+413
+00:31:48,990 --> 00:31:54,910
+Tryptophan هيكشف على هذه الكيمية هذا الفرق بين
+
+414
+00:31:54,910 --> 00:32:03,510
+الالخ reagent و ال Kovacs reagent طيب احنا قلنا
+
+415
+00:32:03,510 --> 00:32:08,190
+اذا بتتذكروا في فحص ال Catalase ال Catalase كان ال
+
+416
+00:32:09,270 --> 00:32:11,690
+الـ substrate اللى بيشتغل عليها الانزيم الـ
+
+417
+00:32:11,690 --> 00:32:17,150
+hydrogen peroxide قولنا في حال ما كان عندي كتالياز
+
+418
+00:32:17,150 --> 00:32:20,190
+في حال البكتيريا ما أفرزت انزيم الكتالياز ال
+
+419
+00:32:20,190 --> 00:32:25,050
+hydrogen peroxide يعتبر toxic فبالتالي البكتيريا
+
+420
+00:32:25,050 --> 00:32:28,870
+اللى كانت negative للكتالياز هتبقى هيكون انت انزيم
+
+421
+00:32:28,870 --> 00:32:31,950
+تانى عشان تتخلص من ال hydrogen peroxide اللى هو ال
+
+422
+00:32:31,950 --> 00:32:38,230
+hydrogen peroxidase قولنا برضه لـ tryptophan عشان
+
+423
+00:32:38,230 --> 00:32:41,670
+اني اناأستفيد منه وأحصل على carbon و nitrogen
+
+424
+00:32:41,670 --> 00:32:44,310
+source في حال البكتيريا ما كان فيه عندها
+
+425
+00:32:44,310 --> 00:32:48,930
+tryptophan S في بكتيريا عندها أنزيم تاني اسمه
+
+426
+00:32:48,930 --> 00:32:54,070
+tryptophan deaminase هذا هيشتغل على نفس ال
+
+427
+00:32:54,070 --> 00:32:57,910
+substrate اللي اشتغل عليها أنزيم ال tryptophan S
+
+428
+00:32:57,910 --> 00:33:02,850
+هيعمل deamination احنا قلنا ال tryptophan بتكون من
+
+429
+00:33:02,850 --> 00:33:07,310
+endol and pyruvate وNH3 مربوطين في بعضفبالتالي
+
+430
+00:33:07,310 --> 00:33:10,850
+انزيم لـ tryptophan D-aminase هيجي يعمل D
+
+431
+00:33:10,850 --> 00:33:16,750
+-amination لـ tryptophan هيرفع الأمانيا لحال هيخل
+
+432
+00:33:16,750 --> 00:33:20,090
+الاندول و البيروفات مربوطين ببعض D-aminase يبقى
+
+433
+00:33:20,090 --> 00:33:24,770
+هيعمل D-amination هيزيل الأمانيا لكن هيخل الاندول
+
+434
+00:33:24,770 --> 00:33:30,630
+و البيروفات مع بعض كيف بدي أكشف عن أنه صارت تفاعل
+
+435
+00:33:30,630 --> 00:33:35,290
+بقولنا أني أنا بدي أضيف reagentعشان يرتبط مع ناتج
+
+436
+00:33:35,290 --> 00:33:39,690
+من نواتج التفاعل في هذه الحالة بضيف reactant اللي
+
+437
+00:33:39,690 --> 00:33:45,030
+هو ال triptofan-d-aminase reactant مكيونته FeCl3
+
+438
+00:33:45,030 --> 00:33:50,150
+زي الـ HCl زي الـ distilled waterمين بالظبط اللى
+
+439
+00:33:50,150 --> 00:33:56,030
+هيرتبط بالنواتج التفاعل من مكونات الـ TDR agent
+
+440
+00:33:56,030 --> 00:34:01,210
+واللي الـ FECL3 أحد مكونات الـ tryptophan D
+
+441
+00:34:01,210 --> 00:34:06,090
+امينازر agent هيرتبط مع الاندول بيروفات اللى هو
+
+442
+00:34:06,090 --> 00:34:11,750
+ناتج من نواتج التفاعل هيعطيني complex اسمه
+
+443
+00:34:11,750 --> 00:34:16,370
+فرقهيدرازون هذا لونه brown shred يبقى النتيجة ال
+
+444
+00:34:16,370 --> 00:34:20,230
+positive هي ال brownshredلكن النتيجة الـ negative
+
+445
+00:34:20,230 --> 00:34:26,070
+لازم تتغير لون الـ media هذا بالنسبة لفحص
+
+446
+00:34:26,070 --> 00:34:31,050
+التريبتوفان D-aminase في حال البكتيريا ما عندها
+
+447
+00:34:31,050 --> 00:34:34,250
+انزيم للتريبتوفان D-aminase هتستخدم انزيم آخر حتى
+
+448
+00:34:34,250 --> 00:34:37,110
+تستفيد من التريبتوفان D-aminase حتى تحصل على
+
+449
+00:34:37,110 --> 00:34:43,070
+carbon و nitrogen source طيب، شو وظيفة الـ HCL-HAN
+
+450
+00:34:43,940 --> 00:34:51,720
+بقولنا ان الـ HCl-Han بيمنع ارتباط لتريبتوفان مع
+
+451
+00:34:51,720 --> 00:34:56,240
+الـ FECL3 هلأ لو البكتيريا ما عندها انزيم
+
+452
+00:34:56,240 --> 00:35:01,300
+لتريبتوفان دي اميناز هيضلوا لتريبتوفان موجود في ال
+
+453
+00:35:01,300 --> 00:35:07,280
+media اضفت رأي جاندي لأحد مكوناته الـ FECL3 بقول
+
+454
+00:35:07,280 --> 00:35:12,240
+لي الـ FECL3 لومقلق الاندوهال بيروفات يعني ماكان
+
+455
+00:35:12,240 --> 00:35:15,540
+انزيم لتريبتوفان دي اميناز موجود اللي هيكسر
+
+456
+00:35:15,540 --> 00:35:19,120
+لتريبتوفان هيروح يرتبط مع ال substrate مع
+
+457
+00:35:19,120 --> 00:35:23,940
+لتريبتوفان ويعطيني لون أحمر أو لون brown shred
+
+458
+00:35:23,940 --> 00:35:28,990
+فبالتالي هيعطيني false positive resultالـ HCl
+
+459
+00:35:28,990 --> 00:35:34,810
+هيمنع هذا الإرتباط فبالتالي هيمنع إنه يتكون لون
+
+460
+00:35:34,810 --> 00:35:38,990
+أحمر نتيجة إرتباط الـ FACL الـ Tree و الـ
+
+461
+00:35:38,990 --> 00:35:42,830
+Tryptophan مع بعض و يعطوني False Positive Result
+
+462
+00:35:42,830 --> 00:35:48,150
+هذا وظيفة الـ HCl في الـ TDA Reagent لكن الـ HCl
+
+463
+00:35:48,150 --> 00:35:52,970
+في الـ COVAX Reagent اللي هو يرفع الـ Complex اللي
+
+464
+00:35:52,970 --> 00:35:54,770
+اتكون في القاعلة الأعلى
+
+465
+00:35:57,990 --> 00:36:02,030
+نجي للسلايدات الاندول test the ability to degrade
+
+466
+00:36:02,030 --> 00:36:06,630
+amino acid to identifiable end product is often
+
+467
+00:36:06,630 --> 00:36:09,330
+used to differentiate among bacteria بقول لي هو
+
+468
+00:36:09,330 --> 00:36:13,450
+هذا الفحص هيكشف على قدرة البكتيريا انها تكسر ال
+
+469
+00:36:13,450 --> 00:36:16,990
+amino acid فبالتالي هيساعدني ان انا افرق بين
+
+470
+00:36:16,990 --> 00:36:20,830
+البكتيريا tryptophan for example is hydrolyzed to
+
+471
+00:36:20,830 --> 00:36:24,850
+endol pyruvic acid and ammonia قولنا من نواتج
+
+472
+00:36:24,850 --> 00:36:30,040
+التفاعل اندولبيروفات و أمونيا هيتكسروا بواسطة
+
+473
+00:36:30,040 --> 00:36:34,160
+التريبتوفاناز لأن التريبتوفان دي أميناز ان دول مع
+
+474
+00:36:34,160 --> 00:36:39,420
+البيروفات هيدلوا و الأمونيا هتكون لحال البيروفيك
+
+475
+00:36:39,420 --> 00:36:42,140
+أسد قلنا انه بيصير metabolized to produce large
+
+476
+00:36:42,140 --> 00:36:45,520
+amount of energy هتكون مصدر للطاقة الأمونيا
+
+477
+00:36:45,520 --> 00:36:50,500
+ستستخدم قلنا عشان أعمل او يتكون new amino acid
+
+478
+00:36:50,500 --> 00:36:55,670
+كمصدر للنيتروجين و الكربون اللازم لتكوين ال amino
+
+479
+00:36:55,670 --> 00:36:59,870
+acid فبالتالي هيتكون لبروتين اللازم لنمو البكتيريا
+
+480
+00:36:59,870 --> 00:37:04,730
+ال end ده اللي باكشف عنه كيف قولنا انه بضيف covax
+
+481
+00:37:04,730 --> 00:37:09,850
+reagent بيتكون من بارا diamethyl amino
+
+482
+00:37:09,850 --> 00:37:14,430
+benzaldehyde in alcohol قولنا هذا هو الإختصار ال B
+
+483
+00:37:15,620 --> 00:37:21,940
+الـ BMAB هذا هي اختصارها أحد مكونات الـ GovEx
+
+484
+00:37:21,940 --> 00:37:26,740
+Reagent تقولنا بترتبط بالاندالي اللي هو أحد نواتج
+
+485
+00:37:26,740 --> 00:37:31,340
+التفاعل هيعطيني اللي هو بالنهاية Complex هذا ال
+
+486
+00:37:31,340 --> 00:37:36,240
+Complex لونه أحمر Cherry Red Ring الـ FCL هيعمل
+
+487
+00:37:36,240 --> 00:37:41,720
+على رفع هذا الراسب لفوق في Lithium هيتكون Red
+
+488
+00:37:41,720 --> 00:37:45,990
+Colorالبرو سيجر انا هعمل incubation بيه لتريبتو
+
+489
+00:37:45,990 --> 00:37:51,010
+لتريبتون البروث RSIM ميديا المهم ميديا بتحتوي على
+
+490
+00:37:51,010 --> 00:37:55,150
+لتريبتوفان اللي هيكون ك substrate هعمل incubation
+
+491
+00:37:55,150 --> 00:37:58,810
+بعد ال incubation هضيف ال covax reagent هعمل
+
+492
+00:37:58,810 --> 00:38:02,550
+shacking و هقرأ immediately هاي الإندول اللي هو
+
+493
+00:38:02,550 --> 00:38:08,540
+ناتج من نواتش عملية تكسير لتريبتوفانهذا الـ Covax
+
+494
+00:38:08,540 --> 00:38:12,180
+Reagent قلنا مكوناته الـ B-dimethyl
+
+495
+00:38:12,180 --> 00:38:16,540
+-aminobenzaldehyde و الـ HCL و الـ aminalcohol
+
+496
+00:38:16,540 --> 00:38:21,820
+بالآخر هيتكوّن لعندي sherry red ring النتيجة قلنا
+
+497
+00:38:21,820 --> 00:38:24,860
+red ring in the top layer indicate the presence of
+
+498
+00:38:24,860 --> 00:38:29,840
+indol لكن في حال غياب اللون هذا بيدل إنه الindol
+
+499
+00:38:29,840 --> 00:38:34,960
+ما اتكون فبالتالي إنزيم لتربطه فناز مش موجود في
+
+500
+00:38:34,960 --> 00:38:39,210
+البكتيرياهذا الفحص ashes significant له و بقولنا
+
+501
+00:38:39,210 --> 00:38:44,430
+بيميز ال E coli اللي هي positive لهذا الفحص هيتكون
+
+502
+00:38:44,430 --> 00:38:49,070
+red ring في ال top from negative اللي negative
+
+503
+00:38:49,070 --> 00:38:52,430
+لهذا الفحص لكلب سلة و ال interior باكتر ما هتكون
+
+504
+00:38:52,430 --> 00:38:57,710
+اي red ring قولنا ال MVEC اللي هو ال Indol و ال
+
+505
+00:38:57,710 --> 00:39:01,270
+Methyl Red Vox Bicarb Citrate هدول الأربع فحوصات
+
+506
+00:39:01,270 --> 00:39:05,740
+يستخدموا للتفريق ال coliform عن بعضلو البكتيريا
+
+507
+00:39:05,740 --> 00:39:10,080
+طلعت lactose fermenter انتيرو بكتيريا لكتاز فرمنتر
+
+508
+00:39:10,080 --> 00:39:12,240
+عشان افرق الـ Coliform اللي هي الـ Eclipsilla
+
+509
+00:39:12,240 --> 00:39:17,160
+سيتروباكتر و الانتيروباكتر و ال E.coli بستخدم أربع
+
+510
+00:39:17,160 --> 00:39:21,640
+فحوصات اللي هم ال Endol Red Vagas Biscuit و
+
+511
+00:39:21,640 --> 00:39:27,900
+السيترات عشان أفرق الأربع الأربع هدول genus عن بعض
+
+512
+00:39:31,190 --> 00:39:36,490
+هك بنكون خلصنا ال test Indole هنتقل لاخر فحص
+
+513
+00:39:36,490 --> 00:39:39,630
+كيميائي اللي هو ال test Nitrate Reduction
+
diff --git a/PL9fwy3NUQKwZWgAb_V1ATlRRsH-6i3cPC/a4LrdmbI9CQ.srt b/PL9fwy3NUQKwZWgAb_V1ATlRRsH-6i3cPC/a4LrdmbI9CQ.srt
new file mode 100644
index 0000000000000000000000000000000000000000..e68bae4f257f5ddab68c609254ce5374b0b890b7
--- /dev/null
+++ b/PL9fwy3NUQKwZWgAb_V1ATlRRsH-6i3cPC/a4LrdmbI9CQ.srt
@@ -0,0 +1,1397 @@
+1
+00:00:00,370 --> 00:00:02,690
+في هاي الـ slide هنتكلم عن بعض النقاط المهمة
+
+2
+00:00:02,690 --> 00:00:06,190
+بالنسبة للـ light compound microscope أول شيء الـ
+
+3
+00:00:06,190 --> 00:00:09,210
+magnification الـ magnification هي قوة التكبير
+
+4
+00:00:09,210 --> 00:00:13,490
+relative enlargement of the specimen هو قوة تكبير
+
+5
+00:00:13,490 --> 00:00:17,130
+العين أو الشريحة حتى أحصل على الـ total
+
+6
+00:00:17,130 --> 00:00:21,530
+magnification بضرب الـ eyepiece magnification ضرب الـ
+
+7
+00:00:21,530 --> 00:00:25,190
+objective magnification قوة التكبير العدسة العينية
+
+8
+00:00:25,190 --> 00:00:29,250
+اللي هي الـ ocular lens magnification اللي هي ثابتة
+
+9
+00:00:29,250 --> 00:00:33,590
+10X ضرب قوة تكبير العدسة الشيئية اللي أنا استخدمتها
+
+10
+00:00:33,590 --> 00:00:40,150
+فيها عندي أربع عدسات شيئية 4x 10x 40x 100x حسب أنا
+
+11
+00:00:40,150 --> 00:00:43,770
+شو استخدمت بضربها بقوة تكبير العدسة العينية حتى
+
+12
+00:00:43,770 --> 00:00:47,510
+أحصل على الـ total magnification the image seen by
+
+13
+00:00:47,510 --> 00:00:50,430
+the eye through a compound microscope is termed
+
+14
+00:00:50,430 --> 00:00:54,580
+the virtual image بقول لك الصورة اللي أنا بحصل عليها
+
+15
+00:00:54,580 --> 00:00:58,080
+من خلال الـ light compound microscope بوصفها بأنها
+
+16
+00:00:58,080 --> 00:01:02,940
+virtual image إيش يعني virtual يعني افتراضية في
+
+17
+00:01:02,940 --> 00:01:07,260
+الوضع الطبيعي أنا لو جبت حشرة بشوفها بثلاثة أبعاد طول
+
+18
+00:01:07,260 --> 00:01:10,420
+وعرض وارتفاع لكن أنا لو روحت جبت هذه الحشرة و
+
+19
+00:01:10,420 --> 00:01:14,740
+حطيتها تحت الـ microscope عشان أشوفها، هأشوف فقط طول و
+
+20
+00:01:14,740 --> 00:01:18,720
+عرض، بالتالي هي صورة افتراضية، صورة virtual مش
+
+21
+00:01:18,720 --> 00:01:24,600
+حقيقية، ما شفتهاش في الوضع الطبيعي، يبقى أول شيء
+
+22
+00:01:24,600 --> 00:01:28,640
+بحكي أن هي عبارة عن virtual image صورة افتراضية
+
+23
+00:01:28,640 --> 00:01:33,060
+and is upside down إيش يعني upside down؟ لو أنا جبت هاي الحشرة
+
+24
+00:01:33,060 --> 00:01:36,600
+لو حطيتها تحت الـ microscope فقط هأشوف اللي على السطح
+
+25
+00:01:37,030 --> 00:01:40,270
+مش هأشوف شيء من تحت، لو بدي أشوف التفاصيل بروح
+
+26
+00:01:40,270 --> 00:01:46,130
+بقطعها زي ما بنقطع في معمل الأنسجة، and reversed
+
+27
+00:01:46,130 --> 00:01:50,970
+إيش يعني reversed؟ يعني معكوسة، الضوء طبعاً لما يمر
+
+28
+00:01:50,970 --> 00:01:54,350
+فيه هيصير له انكسار، لما إحنا نشوف حالنا على المرايا
+
+29
+00:01:54,350 --> 00:01:57,790
+نشوف الصورة بتكون معكوسة لأن الضوء لما يمر عبر
+
+30
+00:01:57,790 --> 00:02:01,510
+الزجاج هينكسر، والعدسات اللي موجودة في الـ
+
+31
+00:02:01,510 --> 00:02:05,670
+microscope مصنوعة من الزجاج بالتالي الضوء هيمر
+
+32
+00:02:05,900 --> 00:02:10,260
+وينكسر ونشوف صورة معكوسة، يبقى الصورة اللي أنا
+
+33
+00:02:10,260 --> 00:02:13,760
+بشوفها بـ light compound microscope virtual صورة
+
+34
+00:02:13,760 --> 00:02:18,000
+افتراضية، and upside down اللي هي فقط بشوف اللي على
+
+35
+00:02:18,000 --> 00:02:28,060
+السطح، and reversed بتكون معكوسة، total
+
+36
+00:02:28,060 --> 00:02:31,120
+magnification تكلمنا، حتى أحصل عليه eyepiece
+
+37
+00:02:31,120 --> 00:02:35,740
+magnification ضرب objective magnification هأقول لك أنه
+
+38
+00:02:35,740 --> 00:02:39,800
+طبعاً العدسة العينية كلها الـ magnification تبعها
+
+39
+00:02:39,800 --> 00:02:44,820
+10x العدسات الشيئية، أنا فيها 4x بسميها scanning
+
+40
+00:02:44,820 --> 00:02:48,660
+objective إيش يعني scanning؟ يعني ماسحة، أنا من
+
+41
+00:02:48,660 --> 00:02:53,660
+خلالها بشوف كل الشريحة، كل slide، بألقي نظرة على كل
+
+42
+00:02:53,660 --> 00:02:57,640
+slide، لو أنا بدي أحصل على الـ total magnification
+
+43
+00:02:57,640 --> 00:03:02,560
+من الـ 4x objective، الـ total magnification يكون 40
+
+44
+00:03:03,060 --> 00:03:07,560
+بالنسبة لو استخدمت 10x objective، بحصل على 100x
+
+45
+00:03:07,560 --> 00:03:11,040
+total magnification، الاسم الثاني لـ الـ 10x
+
+46
+00:03:11,040 --> 00:03:21,300
+objective اللي هي الـ low power، لو
+
+47
+00:03:21,300 --> 00:03:25,220
+أنا استخدمت 40x objective بحصل على total
+
+48
+00:03:25,220 --> 00:03:29,720
+magnification 400x، الاسم الثاني لـ الـ 40x
+
+49
+00:03:29,720 --> 00:03:31,520
+objective، الـ high power
+
+50
+00:03:38,680 --> 00:03:43,780
+يبقى الـ 4X اسمها scanning objective، الـ 10X
+
+51
+00:03:43,780 --> 00:03:49,980
+بسميها low power، والـ 40X بسميها الـ high power
+
+52
+00:03:49,980 --> 00:03:55,960
+في عندي أنا الـ 100X، الـ
+
+53
+00:03:55,960 --> 00:04:04,790
+100X objective، لو أنا بدي أضربها بالـ 10X اللي هي
+
+54
+00:04:04,790 --> 00:04:10,290
+الـ eyepiece، بحصل على Total Magnification بـ 1000X
+
+55
+00:04:10,290 --> 00:04:15,050
+Total Magnification، بالنسبة لـ 100X بسميها oil
+
+56
+00:04:15,050 --> 00:04:22,630
+immersion تمام،
+
+57
+00:04:22,630 --> 00:04:26,890
+الـ 100X بسميها oil immersion، الـ 40X بسميها الـ
+
+58
+00:04:26,890 --> 00:04:30,680
+high power، الـ 10X بسميها الـ low power، الـ 4X ده
+
+59
+00:04:30,680 --> 00:04:33,720
+هأسميها الـ scanning power، هيك أنا حصلت على الـ
+
+60
+00:04:33,720 --> 00:04:40,180
+Total Magnification، خلصنا
+
+61
+00:04:40,180 --> 00:04:43,140
+من الـ Magnification ومن الـ Total Magnification
+
+62
+00:04:43,140 --> 00:04:46,920
+في أنا عندي slide، اللي هي الـ Numerical Aperture
+
+63
+00:04:46,920 --> 00:04:49,960
+لكن قبل ما أتكلم على الـ Numerical Aperture بدي
+
+64
+00:04:49,960 --> 00:04:54,540
+أتكلم عن الـ Resolution، كثير حكينا في الـ
+
+65
+00:04:54,540 --> 00:04:59,190
+Resolution لكن بدنا نعرفه بشكل علمي، الـ Resolution
+
+66
+00:04:59,190 --> 00:05:03,790
+it is the ability of the microscope to distinguish two
+
+67
+00:05:03,790 --> 00:05:08,290
+points يقول لك هو قدرة الـ microscope أو العدسة أنه
+
+68
+00:05:08,290 --> 00:05:12,930
+يميز بين نقطتين، نكمل التعريف أن هاتين النقطتين
+
+69
+00:05:12,930 --> 00:05:18,130
+يكونوا المسافة بينهما قليلة، يبقى that how small
+
+70
+00:05:18,130 --> 00:05:25,910
+the distance between them، نحكي that how small
+
+71
+00:05:31,200 --> 00:05:38,160
+the distance between them
+
+72
+00:05:38,160 --> 00:05:43,540
+هو قدرة الميكروسكوب أو العدسة يميز بين نقطتين
+
+73
+00:05:43,540 --> 00:05:45,840
+المسافة بينهما قليلة
+
+74
+00:05:53,680 --> 00:05:57,080
+The smaller the distance between the two specific
+
+75
+00:05:57,080 --> 00:06:00,940
+points that can be distinguished apart, the
+
+76
+00:06:00,940 --> 00:06:03,960
+greater the resolution power of the microscope
+
+77
+00:06:03,960 --> 00:06:09,060
+و أقول لك كل ما كانت المسافة ما بين جسمين مختلفين
+
+78
+00:06:09,060 --> 00:06:12,800
+و أنا قدرت أميز بين هذين الجسمين، كل ما كانت المسافة
+
+79
+00:06:12,800 --> 00:06:16,740
+الصغيرة و أنا قدرت أميز بين هذين الجسمين كل ما كان
+
+80
+00:06:16,740 --> 00:06:20,670
+الـ resolution power لهذا الـ microscope عالي، يعني لو
+
+81
+00:06:20,670 --> 00:06:23,630
+كان الـ Microscope الـ Resolution تبعه عالي معناه
+
+82
+00:06:23,630 --> 00:06:27,290
+أنا هأقدر أميز بين نقطتين مختلفتين المسافة بينهما
+
+83
+00:06:27,290 --> 00:06:31,070
+كثير قليلة، و أحكي أنه والله هذا جسم X وهذا جسم Y
+
+84
+00:06:31,070 --> 00:06:34,790
+برغم أن المسافة كثير قليلة بينهما لكن لو كان الـ
+
+85
+00:06:34,790 --> 00:06:38,230
+Resolution لـ الـ Microscope أو العدسة كثير قليل
+
+86
+00:06:38,230 --> 00:06:41,930
+النقطتين هاتين هيبينوا كأنهما جسم واحد وهيكونوا فوق
+
+87
+00:06:41,930 --> 00:06:45,090
+بعض، فبالتالي الـ Resolution لـ الـ Microscope
+
+88
+00:06:45,090 --> 00:06:50,590
+هيكون قليل، The larger numerical aperture قبل ما
+
+89
+00:06:50,590 --> 00:06:55,690
+نتكلم عن هذه الجملة هنعرف لـ numerical aperture
+
+90
+00:06:55,690 --> 00:07:09,110
+The
+
+91
+00:07:09,110 --> 00:07:12,790
+numerical aperture: The amount of light reaching
+
+92
+00:07:12,790 --> 00:07:17,090
+the specimen هو كمية الضوء الواصلة للعينة، As
+
+93
+00:07:17,090 --> 00:07:20,090
+numerical aperture increases, the resolution
+
+94
+00:07:20,090 --> 00:07:23,910
+increases، بقول لك كل ما زادت كمية الضوء اللي واصلة
+
+95
+00:07:23,910 --> 00:07:27,390
+للعينة، كل ما كان الـ Resolution للـ Microscope أو
+
+96
+00:07:27,390 --> 00:07:32,930
+العدسة كبيرة، إحنا قلنا لو أنت كنت في 10X أو 4X جبت
+
+97
+00:07:32,930 --> 00:07:35,950
+الـ field و روحتي على الـ high power 40X بتلاقي
+
+98
+00:07:35,950 --> 00:07:40,890
+الصورة مغبشة، وقلنا عشان تكون الصورة واضحة
+
+99
+00:07:40,890 --> 00:07:44,530
+بروح أتحكم بالـ Fine Adjustment، وقلت في بعض الأشياء
+
+100
+00:07:44,530 --> 00:07:48,170
+كمان اللي ممكن أتحكم فيها اللي هنتكلم عنها من ضمن
+
+101
+00:07:48,170 --> 00:07:51,870
+الأمور الثانية اللي أنا بأتحكم فيها كمية الضوء
+
+102
+00:07:51,870 --> 00:07:56,750
+الواصلة اللي هي الـ numerical aperture، طيب أنا كيف
+
+103
+00:07:56,750 --> 00:08:00,890
+بدي أتحكم بالـ numerical aperture؟ كيف بدي أتحكم
+
+104
+00:08:00,890 --> 00:08:04,650
+بكمية الضوء الواصلة خلال العينة؟ اتكلمنا، أجزاء
+
+105
+00:08:04,650 --> 00:08:08,930
+الميكروسكوب، عندي شيء اسمه diaphragm، هذا من خلاله
+
+106
+00:08:08,930 --> 00:08:13,340
+أنا بأتحكم بكمية الضوء الواصلة خلال العينة، بروح أنا
+
+107
+00:08:13,340 --> 00:08:17,440
+عشان أزود الـ numerical aperture بفتح الـ diaphragm
+
+108
+00:08:17,440 --> 00:08:22,580
+شوية، عشان أنا أزيد الـ resolution، تمام، من
+
+109
+00:08:22,580 --> 00:08:26,640
+خلال هاي الجملة هنستنتج علاقة مهمة، أن كل ما زاد الـ
+
+110
+00:08:26,640 --> 00:08:30,920
+magnification كل ما زادت قوة التكبير كل ما احتجت
+
+111
+00:08:30,920 --> 00:08:36,290
+كمية ضوء أكثر حتى أن تصل للعينة، بالتالي الـ
+
+112
+00:08:36,290 --> 00:08:40,350
+Resolution للـ Microscope أو العدسة بيكون كثير
+
+113
+00:08:40,350 --> 00:08:45,750
+أكثر، كثير بيزيد أو بيكون كثير، تمام؟ اللي بيؤكد
+
+114
+00:08:45,750 --> 00:08:49,450
+الكلام، أنه أنا لما كنت في 10X وانتقلت على الـ high
+
+115
+00:08:49,450 --> 00:08:53,070
+power للـ 40X احتجت كمية ضوء أكثر، روحت فتحت
+
+116
+00:08:53,070 --> 00:08:57,310
+diaphragm الـ diaphragm عشان يصل ضوء أكثر للعينة
+
+117
+00:08:57,310 --> 00:09:00,190
+بالتالي الـ Resolution زاد، شو يعني الـ Resolution
+
+118
+00:09:00,190 --> 00:09:03,210
+زاد؟ يعني قدرت أميز بين جسمين المسافة بينهما
+
+119
+00:09:03,210 --> 00:09:07,930
+كثير قليلة، لكن لو أنا كنت على 4X أو على 10X
+
+120
+00:09:07,930 --> 00:09:12,790
+ما أحتاجش كمية ضوء كبيرة، وهذا كثير طالبات وطلاب
+
+121
+00:09:12,790 --> 00:09:16,530
+لما يروحوا يجيبوا الـ field على 4X و 10X بينسوا يسكروا
+
+122
+00:09:16,530 --> 00:09:19,590
+الـ diaphragm، يصيروا يطلعوا كل شوية وينزلوا ويحكوا
+
+123
+00:09:19,590 --> 00:09:23,350
+أنهم ما لقوا الـ field، لكن هو مش ما لقوا الـ field
+
+124
+00:09:23,350 --> 00:09:28,610
+لازم إحنا نسكر الـ diaphragm على عدسة 4 و 10 لأن
+
+125
+00:09:28,610 --> 00:09:31,670
+قلنا في علاقة عكسية بين الـ magnification و الـ
+
+126
+00:09:32,160 --> 00:09:37,600
+numerical aperture، أنا بدي العدسة الأربعة
+
+127
+00:09:37,600 --> 00:09:42,000
+X قوة تكبيرها كثير قليلة، فبالتالي ما أحتاجش كمية ضوء
+
+128
+00:09:42,000 --> 00:09:46,220
+فلابد من إغلاق الديافراغم، نيجي للعلاقة الرياضية
+
+129
+00:09:46,220 --> 00:09:49,980
+الـ Numerical Aperture بيقول لك بيساوي الـ Angular
+
+130
+00:09:49,980 --> 00:09:54,300
+Aperture ضرب الـ Refractive Index، الـ Angular
+
+131
+00:09:54,300 --> 00:09:57,840
+Aperture اللي هي الـ Sin θ ضرب الـ Refractive Index
+
+132
+00:09:57,840 --> 00:10:04,340
+اللي هو الـ n، ما يبقى θ هي عبارة عن زاوية الانكسار لو
+
+133
+00:10:04,340 --> 00:10:15,940
+أنا عندي هاي الـ slide ومرّ الضوء عبر هاي الـ slide
+
+134
+00:10:15,940 --> 00:10:23,200
+الـ slide ومرّ الضوء عبر هاي الـ slide، الضوء لما يمر عبر
+
+135
+00:10:23,200 --> 00:10:29,870
+الـ slide هينكسر بزاوية، هاي الزاوية هي عبارة عن θ
+
+136
+00:10:29,870 --> 00:10:33,970
+«زاوية الانكسار» هي θ، بأعوّض فيها في «refractive
+
+137
+00:10:33,970 --> 00:10:38,510
+index» ومعامل انكسار الوسط الموجود ما بين الـ
+
+138
+00:10:38,510 --> 00:10:41,870
+«slide» وما بين العدسة الشيئية، اللي هنتكلم فيه
+
+139
+00:10:41,870 --> 00:10:45,950
+بآخر الكلام، الـ numerical aperture بيقول لك بيتمد
+
+140
+00:10:45,950 --> 00:10:50,770
+على الـ radius of the lens بيتمد على نصف قطر العدسة
+
+141
+00:10:50,770 --> 00:10:54,510
+والعلاقة ما بين الـ numerical aperture و radius
+
+142
+00:10:54,510 --> 00:10:56,890
+علاقة عكسية، تمام؟
+
+143
+00:11:00,010 --> 00:11:07,370
+نرجع لـ slide، الـ «high» «The larger numerical
+
+144
+00:11:07,370 --> 00:11:11,750
+aperture, the smaller the resolvable distance and
+
+145
+00:11:11,750 --> 00:11:14,990
+hence the more efficient the resolution power»
+
+146
+00:11:17,070 --> 00:11:20,290
+أحتاج كمية ضوء أكثر، كل ما زاد الـ numerical
+
+147
+00:11:20,290 --> 00:11:24,830
+aperture كل ما قدرت أميز بين نقطتين المسافة بينهما
+
+148
+00:11:24,830 --> 00:11:27,870
+أكثر قليلة، بيكون بالتالي بيكون الـ resolution .. الـ
+
+149
+00:11:27,870 --> 00:11:32,110
+resolution لـ الـ microscope أو العدسة أكثر عالي
+
+150
+00:11:32,110 --> 00:11:35,190
+فيعرفنا العلاقة الرياضية عشان نفهم العلاقة
+
+151
+00:11:35,190 --> 00:11:35,870
+الرياضية
+
+152
+00:11:48,610 --> 00:11:55,250
+هنا نكتبها مكتوب
+
+153
+00:11:55,250 --> 00:12:07,930
+في عندي R اللي هو R
+
+154
+00:12:07,930 --> 00:12:16,150
+بيساوي الـ .. في عندي رقم مكتوب
+
+155
+00:12:45,120 --> 00:12:48,500
+الرقم ضرب الـ wavelength على الـ numerical aperture
+
+156
+00:12:57,620 --> 00:13:04,060
+هذه هي العلاقة الرياضية، خلينا نحكي عن كل جزء بهذه
+
+157
+00:13:04,060 --> 00:13:08,680
+العلاقة، R هي عبارة عن مقياس أصغر مسافة قابلة
+
+158
+00:13:08,680 --> 00:13:13,900
+للتمييز بين نقطتين، R هي مقياس يعبر عن distance عن
+
+159
+00:13:13,900 --> 00:13:17,820
+مسافة، أصغر مسافة قابلة للتمييز بين نقطتين، طبعاً هذا
+
+160
+00:13:17,820 --> 00:13:22,020
+ثابت، الـ wavelength هو الطول الموجي، طبعاً إحنا قلنا
+
+161
+00:13:22,020 --> 00:13:26,160
+أن الضوء يمر عبر العينة، فأنا بأخذ طول الموجة اللي هيدخل
+
+162
+00:13:26,160 --> 00:13:29,680
+الضوء اللي بيمر عبر العينة بأعوّض عنه في هذا القانون
+
+163
+00:13:29,680 --> 00:13:33,880
+الـ numerical aperture هي كمية الضوء اللي مرّ عبر
+
+164
+00:13:33,880 --> 00:13:38,580
+العينة، طيب، من هاي العلاقة أنا بأستنتج أن في عندي
+
+165
+00:13:38,580 --> 00:13:43,020
+أو قبل ما نحكي عن هاي العلاقة، هلأ إحنا قلنا كل ما
+
+166
+00:13:43,020 --> 00:13:48,460
+زاد الـ magnification كل ما احتجت كمية الضوء أكثر
+
+167
+00:13:48,460 --> 00:13:52,610
+كل ما زاد الـ magnification كل ما احتجت كمية الضوء
+
+168
+00:13:52,610 --> 00:13:57,350
+أكثر اللي هو الـ numerical aperture أكثر، فبالتالي
+
+169
+00:13:57,350 --> 00:14:02,570
+كل ما كان الـ resolution للميكروسكوب كثير عالي
+
+170
+00:14:02,570 --> 00:14:07,250
+فبالتالي قدرت أميز بين نقطتين المسافة بينهما كثير
+
+171
+00:14:07,250 --> 00:14:12,430
+قليلة، يبقى R هي المسافة اللي بين النقطتين كثير
+
+172
+00:14:12,430 --> 00:14:16,910
+قليلة قدرت أميز بينهم، وكانت كمية الضوء عالية والـ
+
+173
+00:14:16,910 --> 00:14:20,010
+resolution عالية، يبقى لو بنكمل القانون عشان تكون
+
+174
+00:14:20,010 --> 00:14:26,070
+الأمور واضحة أكثر، بنكتب أن الـ R هي مسافة بين
+
+175
+00:14:26,070 --> 00:14:35,550
+نقطتين وهي مقلوب الـ resolution يبقى
+
+176
+00:14:35,550 --> 00:14:44,890
+الواحد
+
+177
+00:14:44,890 --> 00:14:50,750
+على الـ
+
+178
+00:14:50,750 --> 00:14:51,270
+resolution
+
+179
+00:15:21,890 --> 00:15:
+
+223
+00:18:38,420 --> 00:18:42,600
+وشفت فقط مساحة أقل، شوفت high المساحة ونفس الفكرة
+
+224
+00:18:42,600 --> 00:18:47,000
+بالنسبة للـ Oil immersion، هشوف فقط مساحة أقل، هشوف
+
+225
+00:18:47,000 --> 00:18:51,420
+high المساحة فقط، يبقى كانت علاقة عكسية بين الـ
+
+226
+00:18:51,420 --> 00:18:54,920
+Field of Fusion وما بين الـ Magnification
+
+227
+00:19:05,020 --> 00:19:08,720
+as a magnification increases, the resolution also
+
+228
+00:19:08,720 --> 00:19:11,980
+increases، اتفقنا كل ما زاد الـ magnification كل ما
+
+229
+00:19:11,980 --> 00:19:14,940
+زاد الـ resolution، يعني قدرت أميز بين نقطتين
+
+230
+00:19:14,940 --> 00:19:18,580
+المسافة بينهم كتير قليلة، ولو بدنا نوضح أكثر هذه
+
+231
+00:19:18,580 --> 00:19:21,300
+النقطة، لو أنت كنت راكبة سيارة، والسيارة بتمشي
+
+232
+00:19:21,300 --> 00:19:25,880
+بسرعة معينة، كان في ضوء أحمر من مسافة بعيدة، كل ما
+
+233
+00:19:25,880 --> 00:19:29,620
+اقتربتِ، وكل ما قدرتِ أن تميزي أن ضوء هذا عبارة
+
+234
+00:19:29,620 --> 00:19:35,240
+كان عن نصباحين فوق بعض، وبالتالي قدرتِ تميزي هذين
+
+235
+00:19:35,240 --> 00:19:39,180
+الكائنين اللي كانت المسافة بينهم كتير قريبة، أنتِ كل
+
+236
+00:19:39,180 --> 00:19:43,040
+ما قربتِ، معناه بما يقابله في الميكروسكوب، كل ما
+
+237
+00:19:43,040 --> 00:19:46,140
+كبرتي، كل ما زادت الـ Magnification، كل ما قدرتِ
+
+238
+00:19:46,140 --> 00:19:50,260
+أنك تميزي بين جسمين، كائنين المسافة بينهم كتير
+
+239
+00:19:50,260 --> 00:19:53,840
+صغيرة، to achieve a high magnification with good
+
+240
+00:19:53,840 --> 00:19:57,360
+resolution, the objective lens must have a small
+
+241
+00:19:57,360 --> 00:20:02,250
+radius، كلما قلنا أن الـ numerical aperture لها
+
+242
+00:20:02,250 --> 00:20:06,510
+علاقة تعتمد على الـ «Radius of Length»، وأنا قلت
+
+243
+00:20:06,510 --> 00:20:10,170
+أنَّ العلاقة عكسية، نرجع شوية، نرجع للمعلومات، كل ما
+
+244
+00:20:10,170 --> 00:20:13,090
+زاد الـ Magnification، كل ما احتجتِ ضوء أكثر، يعني
+
+245
+00:20:13,090 --> 00:20:16,490
+الـ numerical aperture كان عالي، كل ما كان الـ
+
+246
+00:20:16,490 --> 00:20:19,750
+Resolution عالي، طب أنا قلت الـ numerical aperture
+
+247
+00:20:19,750 --> 00:20:23,810
+علاقتها عكسية مع الـ Radius، يبقى هيكون الـ Radius
+
+248
+00:20:23,810 --> 00:20:26,970
+علاقته عكسية مع كل من الـ Magnification ومع الـ
+
+249
+00:20:26,970 --> 00:20:30,430
+Resolution، يبقى وهذا اللي بيأكد هذا الكلام، يبقى
+
+250
+00:20:30,430 --> 00:20:33,890
+Magnification هي، كل ما زاد، لأنَّ المرق الأبراطور
+
+251
+00:20:33,890 --> 00:20:37,390
+بيزيد، الـ Resolution بيزيد، ويقل الـ Radius، قلت
+
+252
+00:20:37,390 --> 00:20:42,450
+يبقى عدسة 10 X الـ Radius تبعها هيبقى أصغر من عدسة
+
+253
+00:20:42,450 --> 00:20:45,530
+4 X، من هاي اللي اللي علاقة نستنتج هذه
+
+254
+00:20:45,530 --> 00:20:50,410
+المعلومة، shorter wavelength of light provides
+
+255
+00:20:50,410 --> 00:20:54,320
+greater resolution، اتفقنا حسب العلاقة اللي أخذناها
+
+256
+00:20:54,320 --> 00:20:57,000
+الـ «R» اللي هي أنَّه قياس أصغر مسافة بين نقطتين
+
+257
+00:20:57,000 --> 00:21:01,920
+بتساوي 0.612 ضرب الـ «Wavelength» على الـ
+
+258
+00:21:01,920 --> 00:21:05,220
+«Numerical Aperture»، أنَّه في عندي علاقة عكسية ما
+
+259
+00:21:05,220 --> 00:21:08,520
+بين الـ «Resolution» وما بين الـ «Wavelength»، ما
+
+260
+00:21:08,520 --> 00:21:12,300
+بين الطول الموجي، الطول الموجي هو… الطول الموجي
+
+261
+00:21:12,300 --> 00:21:14,700
+هي خاصية في الضوء، حسب شو أنا… الضوء اللي أنا
+
+262
+00:21:14,700 --> 00:21:18,540
+استخدمته، بستخدم الطول الموجي لهذا الضوء، واللي
+
+263
+00:21:18,540 --> 00:21:22,930
+بيأكد هذا الكلام، أنَّه بالميكروسكوب الإلكتروني، أنا بستخدم
+
+264
+00:21:22,930 --> 00:21:27,370
+إلكترونات، الطول الموجي للإلكترونات هي أقل من الطول
+
+265
+00:21:27,370 --> 00:21:30,970
+الموجي للضوء المرئي، عشان هيك الـ resolution للميكروسكوب
+
+266
+00:21:30,970 --> 00:21:34,890
+الإلكتروني بيكون أعلى من الـ resolution للميكروسكوب
+
+267
+00:21:34,890 --> 00:21:40,090
+الضوئي، لأنَّ الـ wavelength للإلكترونات أقل من الـ
+
+268
+00:21:40,090 --> 00:21:42,010
+wavelength للضوء المرئي
+
+269
+00:21:45,220 --> 00:21:48,580
+هنعمل شوية مراجعة على كل العلاقات اللي اتكلمنا
+
+270
+00:21:48,580 --> 00:21:51,840
+عنها، «depth of focus»، الـ «depth of focus» قلنا
+
+271
+00:21:51,840 --> 00:21:56,240
+هو thickness of specimen، هو سمك المجال that can be
+
+272
+00:21:56,240 --> 00:22:00,120
+seen in focus at one time، قلنا كل علاقة عكسية ما
+
+273
+00:22:00,120 --> 00:22:02,520
+بين الـ magnification وما بين الـ depth of focus
+
+274
+00:22:02,520 --> 00:22:05,900
+الـ field of vision هو المساحة اللي أنا بدي أشوفها
+
+275
+00:22:05,900 --> 00:22:09,260
+قلنا أنَّ العلاقة برضه عكسية، يبقى الـ
+
+276
+00:22:09,260 --> 00:22:11,760
+magnification معاكسة للـ depth of focus والـ
+
+277
+00:22:11,760 --> 00:22:15,610
+field of vision، العلاقة عكسية، لكن الـ Magnification
+
+278
+00:22:15,610 --> 00:22:19,750
+مع الـ Resolution ومع الـ numerical aperture كلهم
+
+279
+00:22:19,750 --> 00:22:22,870
+علاقة طردية، كل ما زاد الـ Magnification كل ما
+
+280
+00:22:22,870 --> 00:22:25,730
+احتجتِ كمية ضوء أكثر تصل للعينة، بالتالي الـ
+
+281
+00:22:25,730 --> 00:22:29,050
+Resolution بيزيد، هاي ملخص كل العلاقات اللي اتكلمنا
+
+282
+00:22:29,050 --> 00:22:33,290
+فيها، الـ Medium between the objective and the
+
+283
+00:22:33,290 --> 00:22:36,370
+object is a factor that must be taken into
+
+284
+00:22:36,370 --> 00:22:39,310
+consideration for the most effective use of
+
+285
+00:22:39,310 --> 00:22:44,110
+microscope lens، بقول لك الوسط بين العدسة الشيئية وما
+
+286
+00:22:44,110 --> 00:22:48,410
+بين العينة لازم ناخدها بعين الاعتبار حتى تكون
+
+287
+00:22:48,410 --> 00:22:52,030
+الصورة واضحة، أنا قلت كل ما زاد الـ Magnification
+
+288
+00:22:52,030 --> 00:22:56,750
+كل ما زاد الـ Resolution، لكن احنا قلنا لازم أحياناً
+
+289
+00:22:56,750 --> 00:23:00,930
+أن ناخذ بعين الاعتبار أمور منها الـ Fine
+
+290
+00:23:00,930 --> 00:23:05,570
+Adjustment، لما اروح من 4X اروح لـ 40X لازم ألعب
+
+291
+00:23:05,570 --> 00:23:09,370
+بالـ Fine Adjustment، لازم أتحكم بكمية الضوء
+
+292
+00:23:09,370 --> 00:23:12,890
+الواصلة لهذه العينة، من الأمور المهمة اللي لازم
+
+293
+00:23:12,890 --> 00:23:18,130
+آخذها بعين الاعتبار، منها برضه الوسط ما بين العدسة
+
+294
+00:23:18,130 --> 00:23:22,030
+الشيئية وما بين الـ Slide، بقول لك The Lower Power
+
+295
+00:23:22,030 --> 00:23:27,150
+Objective 4X, 10X and High Dry Objective 40X use
+
+296
+00:23:27,150 --> 00:23:31,910
+Air، احنا قلنا أن الـ 4X اسمها Scanning Objective
+
+297
+00:23:31,910 --> 00:23:36,560
+لكن هنا هو مسميها Low Power، لكن إحنا خلّينا نتفق
+
+298
+00:23:36,560 --> 00:23:41,080
+أن الـ 4X هي تعتبر Scanning، الـ 10X تعتبر Low
+
+299
+00:23:41,080 --> 00:23:45,000
+Power، لكن لو أنا سميت الـ 4X Low Power عادي، هي
+
+300
+00:23:45,000 --> 00:23:48,680
+Scanning أو Low Power، ولكن الـ High Power هي الـ
+
+301
+00:23:48,680 --> 00:23:53,180
+40X، أقول لك في العدسة الشيئية 4X وفي العدسة الشيئية
+
+302
+00:23:53,180 --> 00:23:57,700
+10X و40X، باستخدام يكون الوسط ما بين العدسة الشيئية
+
+303
+00:23:57,700 --> 00:24:02,020
+وما بين الـ Slide يكون الهواء، لكن عند العدسة 100X
+
+304
+00:24:02,930 --> 00:24:05,930
+الـ Oil Immersion، بستخدم الـ Oil عشان هي، احنا
+
+305
+00:24:05,930 --> 00:24:10,090
+بنسميها Oil Immersion لأن بنضيف الـ Oil، طيب ليش
+
+306
+00:24:10,090 --> 00:24:15,410
+أنا بضيف Oil عند 100X؟ بقول لك لأن الـ Oil نفس
+
+307
+00:24:15,410 --> 00:24:20,690
+معامل الانكسار للزجاج، حلو، لما ينتقل الضوء من بين وسطين معامل
+
+308
+00:24:20,690 --> 00:24:24,870
+انكسارهما مختلف، بيتشتت الضوء، بيتشتت الضوء يعني الـ
+
+309
+00:24:24,870 --> 00:24:28,830
+numerical aperture، يعني كمية الضوء الواصلة للعينة
+
+310
+00:24:28,830 --> 00:24:32,900
+بتكون قليلة، وبالتالي الـ Resolution هيقل، وضوح الصورة
+
+311
+00:24:32,900 --> 00:24:36,640
+هيقل، عشان هيك أنا بستخدم الـ Oil اللي له نفس
+
+312
+00:24:36,640 --> 00:24:41,200
+معامل الانكسار للزجاج، وبالتالي مش هيتشتت الضوء، هيتجمع على
+
+313
+00:24:41,200 --> 00:24:45,640
+العدسة، هيوصل كمية كبيرة من الضوء للعينة، numerical
+
+314
+00:24:45,640 --> 00:24:48,700
+aperture هيكون عالي، بالتالي هيكون الـ Resolution
+
+315
+00:24:48,700 --> 00:24:54,500
+وضوح الصورة أكثر، في حالة أن أنا لو ضفت Oil، تمام،
+
+316
+00:24:54,500 --> 00:24:57,540
+هذا بالنسبة للسبب أني بحط Oil، when oil
+
+317
+00:24:57,540 --> 00:25:00,400
+immersion lenses are employed, a drop of oil should
+
+318
+00:25:00,400 --> 00:25:02,820
+be used، أقول لك، لو أنا استخدمت الـ Oil immersion
+
+319
+00:25:02,820 --> 00:25:07,780
+lenses، اللي احنا سميناها هي الـ 100X Objective
+
+320
+00:25:07,780 --> 00:25:12,740
+بقول لك، نضيف نقطة من الزيت، وليه هذا السبب؟
+
+321
+00:25:12,740 --> 00:25:15,520
+binding of the light waves and currents، لأن
+
+322
+00:25:15,520 --> 00:25:20,830
+بيصير في عندي انحناء للضوء، Oil has the same
+
+323
+00:25:20,830 --> 00:25:24,310
+refractive index of the glass, while air in the
+
+324
+00:25:24,310 --> 00:25:28,030
+case of refraction، لأن معامل الانكسار ذات، إنه نفس
+
+325
+00:25:28,030 --> 00:25:32,330
+معامل الانكسار للزجاج، لكن بالنسبة للهواء معامل
+
+326
+00:25:32,330 --> 00:25:37,190
+انكسار الهواء أقل من الزجاج، وهذا بيؤدي إلى أنَّه
+
+327
+00:25:37,190 --> 00:25:41,690
+يتشتت الضوء أكثر، وتقل كمية الضوء الواصلة للعينة
+
+328
+00:25:41,690 --> 00:25:43,450
+بالتالي الـ Resolution تقل
+
+329
+00:25:47,540 --> 00:25:52,600
+هنحطلك صورة مقارنة بين، لو أنا بدون ما أحط oil على
+
+330
+00:25:52,600 --> 00:25:57,580
+100X ولو أنا حطيت oil على العدسة 100X، لو أنا ما
+
+331
+00:25:57,580 --> 00:26:01,920
+حطيت oil على العدسة 100X، بقول لك أنَّه لما يمر الضوء،
+
+332
+00:26:01,920 --> 00:26:07,100
+الضوء هيتشتت، فبالتالي مش هيوصل كمية كبيرة للعينة
+
+333
+00:26:07,100 --> 00:26:11,700
+مش هيوصل كمية كبيرة من الضوء للعينة أو للعدسة
+
+334
+00:26:11,700 --> 00:26:15,300
+الشيئية، فبالتالي الـ Resolution بيقل، لكن لو أنا
+
+335
+00:26:15,300 --> 00:26:19,380
+استخدمت الـ Oil، وبالتالي الضوء مش هيتشتت، هيتجمع على
+
+336
+00:26:19,380 --> 00:26:24,000
+العدسة الشيئية، هيوصل كمية ضوء كبيرة، فبالتالي الـ
+
+337
+00:26:24,000 --> 00:26:29,140
+resolution، وضوح الصورة بيزيد، Refractive إيش يعني؟
+
+338
+00:26:29,140 --> 00:26:32,420
+Refractive يعني bending، انكسار أو انحناء
+
+339
+00:26:32,420 --> 00:26:35,240
+Refractive index is a measurement of the extent
+
+340
+00:26:35,240 --> 00:26:39,440
+that a substance bends light، هو بيقول لك بيقيس معدل
+
+341
+00:26:39,440 --> 00:26:45,220
+انكسار الضوء، field of view or equal، لو كان اللي هو
+
+342
+00:26:45,220 --> 00:26:48,060
+الـ total magnification، field of view يعني الـ
+
+343
+00:26:48,060 --> 00:26:51,140
+total magnification، بقول لك لو كان أكبر أو يساوي
+
+344
+00:26:51,140 --> 00:26:56,000
+500X، causes increase in diffraction and this causes
+
+345
+00:26:56,000 --> 00:26:59,300
+distortion of the image، مش معناه، هلأ أنا لما
+
+346
+00:26:59,300 --> 00:27:02,960
+أستخدم 4X، قلنا معامل الانكسار أو total، sorry
+
+347
+00:27:02,960 --> 00:27:07,700
+total magnification بكون 40X، لو أنا استخدمت 10X
+
+348
+00:27:07,700 --> 00:27:12,710
+بكون اللي هي 100X total magnification، لو أنا استخدمت
+
+349
+00:27:12,710 --> 00:27:18,210
+40X، الـ total magnification بيكون 400X، طيب لو أنا
+
+350
+00:27:18,210 --> 00:27:22,050
+استخدمت 100X، بيكون الـ total magnification 1000X،
+
+351
+00:27:22,050 --> 00:27:26,190
+وين الـ total magnification بيكون أعلى من 500X لو
+
+352
+00:27:26,190 --> 00:27:30,130
+أنا استخدمت اللي هي الـ 100X Objective، لو أنا
+
+353
+00:27:30,130 --> 00:27:33,750
+استخدمت عدسة، العدسة الشيئية 100X هيكون الـ total
+
+354
+00:27:33,750 --> 00:27:38,440
+Magnification أكثر من 500X، فبالتالي في العدسة
+
+355
+00:27:38,440 --> 00:27:43,060
+الشيئية 100X، بيقول لك بيزيد، لما يكون الـ total
+
+356
+00:27:43,060 --> 00:27:46,640
+Magnification أكثر من 500X، اللي هو عند 100X، بيزيد
+
+357
+00:27:46,640 --> 00:27:51,360
+تشطط الضوء، فبالتالي عشان هيك أنا بوضع… بحط oil
+
+358
+00:27:51,360 --> 00:27:54,180
+عند الـ 100X، لأن الـ total magnification اللي
+
+359
+00:27:54,180 --> 00:27:58,340
+بنحصل عليها أكثر من 500X، بالتالي هيكون عند الـ numerical
+
+360
+00:27:58,340 --> 00:28:01,340
+aperture هيتشتت الضوء، الـ numerical aperture هيكون
+
+361
+00:28:01,340 --> 00:28:05,490
+قليل، الـ resolution قليل، بالتالي هذا يسبب
+
+362
+00:28:05,490 --> 00:28:08,430
+Distortion of the image، هيكون في عندي قلة في
+
+363
+00:28:08,430 --> 00:28:11,710
+وضوح الصورة، وقلنا ليش؟ لأنه اتشتت الضوء، ما تجمعش
+
+364
+00:28:11,710 --> 00:28:15,350
+على العينة،  Concerning numerical aperture، هذا
+
+365
+00:28:15,350 --> 00:28:19,210
+بالنسبة للعلاقات
+
+366
+00:28:19,210 --> 00:28:23,410
+المهمة، بالنسبة للـ light compound microscope، هلأ
+
+367
+00:28:23,410 --> 00:28:26,650
+بقول لك كيف أنا أستخدم الـ Microscope، أول شيء طبعاً
+
+368
+00:28:26,650 --> 00:28:32,570
+بحط الفيشة في الكهرباء، بضوي مصدر الإضاءة، وبحط على
+
+369
+00:28:32,570 --> 00:28:37,490
+4X، ببدأ أنا بالـ Low Power، وبعدين بنتقل لـ High
+
+370
+00:28:37,490 --> 00:28:42,190
+Power، بحط على 4X Objective، بحط الـ Smear على
+
+371
+00:28:42,190 --> 00:28:44,590
+الـ Stage، الـ Smear يعني أني أنا بحط الـ Slide أو
+
+372
+00:28:44,590 --> 00:28:48,030
+العينة اللي أنا بديها على الـ Stage، وبشوفها على
+
+373
+00:28:48,030 --> 00:28:52,870
+الـ 4X، بستخدم الـ Course Adjustment Knob، احنا قلنا أن
+
+374
+00:28:52,870 --> 00:28:55,970
+الـ course adjustment knob، حتى أني أستخدمه حتى
+
+375
+00:28:55,970 --> 00:29:01,170
+أن أجيب الـ field، بحركه طبعاً، ممكن أني أحط الـ
+
+376
+00:29:01,170 --> 00:29:06,010
+Stage أخليها للآخر اللي فوق، وأبدأ أني أنزل، أو
+
+377
+00:29:06,010 --> 00:29:09,530
+أني أنزلها للآخر، وأبدأ أطلع شوية شوية، حتى أني
+
+378
+00:29:09,530 --> 00:29:12,630
+أشوف الصورة اللي أنا بديها، هيك معناه أني أنا
+
+379
+00:29:12,630 --> 00:29:16,650
+جبت الـ field، بقول لك بعدين بتنتقلي للـ «objective
+
+380
+00:29:16,650 --> 00:29:20,690
+lens» الثانية، العشرة X وما بعدها اللي هي الأربعين
+
+381
+00:29:20,690 --> 00:29:25,530
+X، وآخر شيء بتوصلي، طبعاً لما توصلي لأربعين، بتحاولي أنك
+
+382
+00:29:25,530 --> 00:29:28,650
+تحركي الـ Fine Adjustment حتى تكون
+
+383
+00:29:28,650 --> 00:29:32,510
+الـ Resolution للـ Image واضحة، لما تستخدمي الـ Oil
+
+384
+00:29:32,510 --> 00:29:35,930
+Immersion، بقول لك بتحطي Drop of Oil، واتفقنا ليش
+
+385
+00:29:35,930 --> 00:29:39,890
+بأننا بنحط Oil على الـ 100X، كافي، بتمسكي
+
+386
+00:29:39,890 --> 00:29:43,740
+الـ Microscope، بقول لك بتمسكيه، إيد بتمسك الـ Arm
+
+387
+00:29:43,740 --> 00:29:46,760
+وإيد ثانية بتمسك الـ Base، زي ما إحنا شايفين أنَّه
+
+388
+00:29:46,760 --> 00:29:50,440
+هان، إيد ماسكة الـ Base قاعدة، والإيد الثانية ماسكة
+
+389
+00:29:50,440 --> 00:29:55,360
+الـ Arm، في بعض العوامل اللي بتأثر على جودة الصورة
+
+390
+00:29:55,360 --> 00:29:58,860
+Quality of Microscope image، الـ lenses اتكلمنا عن هذا
+
+391
+00:29:58,860 --> 00:30:03,460
+الموضوع، أنَّه جودة الـ Microscope والعدسات طبعاً
+
+392
+00:30:03,460 --> 00:30:07,700
+مثلاً على سبيل المثال الـ Microscope الإلكتروني، الـ
+
+393
+00:30:07,700 --> 00:30:
\ No newline at end of file
diff --git a/PL9fwy3NUQKwZWgAb_V1ATlRRsH-6i3cPC/a4LrdmbI9CQ_raw.srt b/PL9fwy3NUQKwZWgAb_V1ATlRRsH-6i3cPC/a4LrdmbI9CQ_raw.srt
new file mode 100644
index 0000000000000000000000000000000000000000..c8a2056f9ef6f89102cff87957807476e2dc51c0
--- /dev/null
+++ b/PL9fwy3NUQKwZWgAb_V1ATlRRsH-6i3cPC/a4LrdmbI9CQ_raw.srt
@@ -0,0 +1,1768 @@
+1
+00:00:00,370 --> 00:00:02,690
+في هاي ال slide هنتكلم عن بعض النقاط المهمة
+
+2
+00:00:02,690 --> 00:00:06,190
+بالنسبة لل light compound microscope أول شئ ال
+
+3
+00:00:06,190 --> 00:00:09,210
+magnification ال magnification هي قوة التكبير
+
+4
+00:00:09,210 --> 00:00:13,490
+relative enlargement of the specimen هو قوة تكبير
+
+5
+00:00:13,490 --> 00:00:17,130
+العين أو الشريحة حتى أحصل على ال total
+
+6
+00:00:17,130 --> 00:00:21,530
+magnification بضرب ال Ibs magnification ضرب ال
+
+7
+00:00:21,530 --> 00:00:25,190
+objective magnification قوة التكبير العدسة العينية
+
+8
+00:00:25,190 --> 00:00:29,250
+اللي هي الocular lens magnification اللي هي ثابت
+
+9
+00:00:29,250 --> 00:00:33,590
+10Xدرب قوة تكبير العدسة الشيئية اللي أنا استخدمتها
+
+10
+00:00:33,590 --> 00:00:40,150
+فيها عندي أربع عدسات شيئية 4x 10x 40x 100x حسب أنا
+
+11
+00:00:40,150 --> 00:00:43,770
+شو استخدمت بضربها بقوة تكبير العدسة العينية حتى
+
+12
+00:00:43,770 --> 00:00:47,510
+أحصل على ال total magnification the image seen by
+
+13
+00:00:47,510 --> 00:00:50,430
+the eye through a compound microscope is termed
+
+14
+00:00:50,430 --> 00:00:54,580
+the virtualimage بقولك الصورة اللي انا بحصل عليها
+
+15
+00:00:54,580 --> 00:00:58,080
+من خلال ال light compound microscope بوصفها بإنها
+
+16
+00:00:58,080 --> 00:01:02,940
+virtual image اش يعني virtual يعني افتراضية في
+
+17
+00:01:02,940 --> 00:01:07,260
+الوضع الطبيعي انا لو جبت حشرة بشوفها ب3 أبعاد طول
+
+18
+00:01:07,260 --> 00:01:10,420
+و عرض و ارتفاع لكن انا لو روحت جبت هذه الحشرة و
+
+19
+00:01:10,420 --> 00:01:14,740
+حطيتها تحت ال microscope عشان اشوفهاهشوف فقط طول و
+
+20
+00:01:14,740 --> 00:01:18,720
+عرض بالتالي هي صورة افتراضية صورة virtual مش
+
+21
+00:01:18,720 --> 00:01:24,600
+حقيقية ماشوفتها بيه الوضع الطبيعي يبقى اول اشي
+
+22
+00:01:24,600 --> 00:01:28,640
+بحكي ان هي عبارة عن virtual image صورة افتراضية
+
+23
+00:01:28,640 --> 00:01:33,060
+and is upside شو يعني upside لو انا جبت هاي الحشة
+
+24
+00:01:33,060 --> 00:01:36,600
+لو حطتها تحت ال microscope فقط هشوف اللي على السطح
+
+25
+00:01:37,030 --> 00:01:40,270
+مش هشوف اشي من تحت لو بدي اشوف التفاصيل بروح
+
+26
+00:01:40,270 --> 00:01:46,130
+بقطحها زي ما بنقطع في معمل الانسجار and reversed
+
+27
+00:01:46,130 --> 00:01:50,970
+اش يعني reversed يعني معكوسة الضوء طبعا لما يمر
+
+28
+00:01:50,970 --> 00:01:54,350
+هيصيرله انكسار لما احنا نشوف حالنا على المرايا
+
+29
+00:01:54,350 --> 00:01:57,790
+نشوف الصورة بتكون معكوسة لأن الضوء لما يمر عبر
+
+30
+00:01:57,790 --> 00:02:01,510
+الزجاج هينكسر و العدسات اللي موجودة في ال
+
+31
+00:02:01,510 --> 00:02:05,670
+microscope مصموعة من الزجاج بالتالي الضوء هيمر
+
+32
+00:02:05,900 --> 00:02:10,260
+وينكسر ونشوف صورة معكوسة يبقى الصورة اللي أنا
+
+33
+00:02:10,260 --> 00:02:13,760
+بشوفها بـ light compound microscope virtual صورة
+
+34
+00:02:13,760 --> 00:02:18,000
+افتراضية and upside اللي هي فقط بشوف اللي على
+
+35
+00:02:18,000 --> 00:02:28,060
+السطح and reverse بتكون معكوسة total
+
+36
+00:02:28,060 --> 00:02:31,120
+magnification اتكلمنا حتى احصل عليه Ibs
+
+37
+00:02:31,120 --> 00:02:35,740
+magnification ضرب objective magnificationهزكلك انه
+
+38
+00:02:35,740 --> 00:02:39,800
+طبعا العدسة العينية كلها ال magnification تبعها
+
+39
+00:02:39,800 --> 00:02:44,820
+10x العدسات الشيئية انا فيها 4x بسميها scanning
+
+40
+00:02:44,820 --> 00:02:48,660
+objective ايش يعني scanning يعني ماسحة انا من
+
+41
+00:02:48,660 --> 00:02:53,660
+خلالها بشوف كل الشريحة كل slide بلقي نظرة على كل
+
+42
+00:02:53,660 --> 00:02:57,640
+slide لو انا بدي احصل على ال total magnification
+
+43
+00:02:57,640 --> 00:03:02,560
+من ال 4x objective ال total magnification يكون 40
+
+44
+00:03:03,060 --> 00:03:07,560
+بالنسبة لو استخدمت 10x objective بحصل على 100x
+
+45
+00:03:07,560 --> 00:03:11,040
+total magnification الاسم التاني لأ ال 10x
+
+46
+00:03:11,040 --> 00:03:21,300
+objective اللي هي ال law power لو
+
+47
+00:03:21,300 --> 00:03:25,220
+أنا استخدمت 40x objective بحصل على total
+
+48
+00:03:25,220 --> 00:03:29,720
+magnification 400x الاسم التاني لأ ال 40x
+
+49
+00:03:29,720 --> 00:03:31,520
+objective ال high power
+
+50
+00:03:38,680 --> 00:03:43,780
+يبقى الـ 4X اسمها scanning objective الـ 10X
+
+51
+00:03:43,780 --> 00:03:49,980
+بسميها low power و الـ 40X بسميها الـ high power
+
+52
+00:03:49,980 --> 00:03:55,960
+في عندي أنا الـ 100X الـ
+
+53
+00:03:55,960 --> 00:04:04,790
+100X objective لو أنا بدي أضربها بالـ 10Xاللي هي
+
+54
+00:04:04,790 --> 00:04:10,290
+الـ I piece بحصل على Total Magnification بـ 1000X
+
+55
+00:04:10,290 --> 00:04:15,050
+Total Magnification بالنسبة لـ 100X بسميها Oil
+
+56
+00:04:15,050 --> 00:04:22,630
+Emergent تمام،
+
+57
+00:04:22,630 --> 00:04:26,890
+الـ 100X بسميها Oil Emergent الـ 40X بسميها الـ
+
+58
+00:04:26,890 --> 00:04:30,680
+High Power الـ 10X بسميها الـ Low Powerالـ 4X ده
+
+59
+00:04:30,680 --> 00:04:33,720
+هسميها الـ Scanning Power هيك أنا حصلت على الـ
+
+60
+00:04:33,720 --> 00:04:40,180
+Total Magnification خلصنا
+
+61
+00:04:40,180 --> 00:04:43,140
+من الـ Magnification ومن الـ Total Magnification
+
+62
+00:04:43,140 --> 00:04:46,920
+في أنا عندي Slide اللي هي الـ Numerical Aperture
+
+63
+00:04:46,920 --> 00:04:49,960
+لكن قبل ما أتكلم على الـ Numerical Aperture بدي
+
+64
+00:04:49,960 --> 00:04:54,540
+أتكلم عن الـ Resolution كثير حكينا في الـ
+
+65
+00:04:54,540 --> 00:04:59,190
+Resolution لكن بدنا نعرفه بشكل علميالـ Resolution
+
+66
+00:04:59,190 --> 00:05:03,790
+it is the ability of microscope to distinguish two
+
+67
+00:05:03,790 --> 00:05:08,290
+points يقولك هو قدرة ال microscope أو العدسة أنه
+
+68
+00:05:08,290 --> 00:05:12,930
+يميز بين نقطتين نكمل التعريف أن هدول النقطتين
+
+69
+00:05:12,930 --> 00:05:18,130
+يكونوا المسافة بينهم قليلة يبقى that how small
+
+70
+00:05:18,130 --> 00:05:25,910
+distance between it نحكي that how small
+
+71
+00:05:31,200 --> 00:05:38,160
+distance between them
+
+72
+00:05:38,160 --> 00:05:43,540
+هو قدرة الميكروسكوب أو العدسة يميز بين نقطتين
+
+73
+00:05:43,540 --> 00:05:45,840
+المسافة بينهم قليلة
+
+74
+00:05:53,680 --> 00:05:57,080
+The smaller the distance between the two specific
+
+75
+00:05:57,080 --> 00:06:00,940
+objectives that can be distinguished apart, the
+
+76
+00:06:00,940 --> 00:06:03,960
+greater the resolution power of the microscope
+
+77
+00:06:03,960 --> 00:06:09,060
+وقولّك كل ما كانت المسافة ما بين جثمان مختلفان
+
+78
+00:06:09,060 --> 00:06:12,800
+وانا قدرت اميز بين هدول الجثمان كل ما كانت المسافة
+
+79
+00:06:12,800 --> 00:06:16,740
+الصغيرة وانا قدرت اميز بين هدول الجثمان كل ما كان
+
+80
+00:06:16,740 --> 00:06:20,670
+ال resolution power لهذا ال microscope عالييعني لو
+
+81
+00:06:20,670 --> 00:06:23,630
+كان الـ Microscope الـ Resolution تبعه عالي معناه
+
+82
+00:06:23,630 --> 00:06:27,290
+أنا هقدر أميز بين نقطتين مختلفتين المسافة بينهم
+
+83
+00:06:27,290 --> 00:06:31,070
+كتير قليلة و أحكي إنه والله هذا جسم X و هذا جسم Y
+
+84
+00:06:31,070 --> 00:06:34,790
+برغم إن المسافة كتير قليلة بينهم لكن لو كان الـ
+
+85
+00:06:34,790 --> 00:06:38,230
+Resolution لأ الـ Microscope أو العدسة كتير قليل
+
+86
+00:06:38,230 --> 00:06:41,930
+النقطتين هدول هيبينوا كأنهم جسم واحد و هيكونوا فوق
+
+87
+00:06:41,930 --> 00:06:45,090
+بعض فبالتالي الـ Resolution لأ الـ Microscope
+
+88
+00:06:45,090 --> 00:06:50,590
+هيكون قليلThe larger enumerical aperture قبل ما
+
+89
+00:06:50,590 --> 00:06:55,690
+نتكلم عن هذه الجملة هنعرف لـ numerical aperture
+
+90
+00:06:55,690 --> 00:07:09,110
+The
+
+91
+00:07:09,110 --> 00:07:12,790
+enumerical aperture The amount of light reaching
+
+92
+00:07:12,790 --> 00:07:17,090
+the specimen هو كمية الضوء الواصلة للعينةAs
+
+93
+00:07:17,090 --> 00:07:20,090
+numerical aperture increases, the resolution
+
+94
+00:07:20,090 --> 00:07:23,910
+increases بقولّك كل ما زادت كمية الضوء اللي واصلة
+
+95
+00:07:23,910 --> 00:07:27,390
+للعينة كل ما كان الـ Resolution للـ Microscope أو
+
+96
+00:07:27,390 --> 00:07:32,930
+العدسة كبيرة احنا قلنا لو انت كنت في 10X او 4X جبت
+
+97
+00:07:32,930 --> 00:07:35,950
+ال field و روحتي على ال high power 40X بتلاقي
+
+98
+00:07:35,950 --> 00:07:40,890
+الصورة مغبشة وقولنا عشان انا تكون الصورة واضحة
+
+99
+00:07:40,890 --> 00:07:44,530
+بروح اتحكم بالـ Fine Adjustmentوقلت في بعض الأشياء
+
+100
+00:07:44,530 --> 00:07:48,170
+كمان اللي ممكن أتحكم فيها اللي هنتكلم عنها من ضمن
+
+101
+00:07:48,170 --> 00:07:51,870
+الأمور التانية اللي أنا بتحكم فيها كمية الضوء
+
+102
+00:07:51,870 --> 00:07:56,750
+المرة اللي هي الـ numerical aperture طيب أنا كيف
+
+103
+00:07:56,750 --> 00:08:00,890
+بدي أتحكم بالـ numerical aperture؟ كيف بدي أتحكم
+
+104
+00:08:00,890 --> 00:08:04,650
+بكمية الضوء المرة خلال العينة؟ اتكلمنات أجزاء
+
+105
+00:08:04,650 --> 00:08:08,930
+الميكروسكوفي عندي إشي اسمه diaphragم هذا من خلاله
+
+106
+00:08:08,930 --> 00:08:13,340
+أنا بتحكم بكمية الضوء المرة خلال العينةفبروح انا
+
+107
+00:08:13,340 --> 00:08:17,440
+عشان ازود ال numerical aperture بفتح ال diaphragm
+
+108
+00:08:17,440 --> 00:08:22,580
+شوية عشان انا عندى ايزية ال resolution تمام من
+
+109
+00:08:22,580 --> 00:08:26,640
+خلال هاي الجملة هنستنتج علاقة مهمة ان كل ما زاد ال
+
+110
+00:08:26,640 --> 00:08:30,920
+magnification كل ما زادت قوة التكبير كل ماحتجت
+
+111
+00:08:30,920 --> 00:08:36,290
+كمية الضوء اكتر حتى انها تصل للعينةبالتالي الـ
+
+112
+00:08:36,290 --> 00:08:40,350
+Resolution للـ Microscope أو العدسة بيكون كتير
+
+113
+00:08:40,350 --> 00:08:45,750
+اكتر كتير بيزيد أو بيكون كتير تمام؟ اللي بيأكد
+
+114
+00:08:45,750 --> 00:08:49,450
+الكلام انه انا لما كنت في 10X وانتقلت على الـ High
+
+115
+00:08:49,450 --> 00:08:53,070
+Power للـ 40X احتجت كمية ضوء اكتر روحت فتحت
+
+116
+00:08:53,070 --> 00:08:57,310
+Diagram الـ Diaphragm عشان يصل ضوء اكتر للعينة
+
+117
+00:08:57,310 --> 00:09:00,190
+بالتالي الـ Resolution زاد شو يعني الـ Resolution
+
+118
+00:09:00,190 --> 00:09:03,210
+زاد؟ يعني اقدرت اميز بين جثمان المسافة بينهم
+
+119
+00:09:03,210 --> 00:09:07,930
+الكتير قليلةلكن لو أنا كنت على 4X أو على 10X
+
+120
+00:09:07,930 --> 00:09:12,790
+مابحتاش كمية الضوء كبيرة وهذا كتير طالبات و طلاب
+
+121
+00:09:12,790 --> 00:09:16,530
+لما يروح يجيبوا ال field على 4X و 10X بنسوا يسكوا
+
+122
+00:09:16,530 --> 00:09:19,590
+ال diaphragm يصيروا يطلعوا كلستاج و ينزلوا و يحكوا
+
+123
+00:09:19,590 --> 00:09:23,350
+انهم مالقوا ال field لكن هو مش مالقوا ال field
+
+124
+00:09:23,350 --> 00:09:28,610
+لازم احنا نسكر ال diaphragm على عدسة 4 و 10 لأن
+
+125
+00:09:28,610 --> 00:09:31,670
+قلنا في علاقة تربية بين ال magnification و المباني
+
+126
+00:09:32,160 --> 00:09:37,600
+الـ Numerical Aperture أنا بدي الكنة العدسة أربعة
+
+127
+00:09:37,600 --> 00:09:42,000
+X قوة تتكبير كتير قليلة فبالتالي ما بحتاج كمية ضوء
+
+128
+00:09:42,000 --> 00:09:46,220
+فلازم يتسكر الديافراج نيجي لها العلاقة الرياضية
+
+129
+00:09:46,220 --> 00:09:49,980
+الـ Numerical Aperture بيقولك بيساوي الـ Angular
+
+130
+00:09:49,980 --> 00:09:54,300
+Aperture ضرب الـ Refractive Index الـ Angular
+
+131
+00:09:54,300 --> 00:09:57,840
+Aperture اللي هي الـ Sinθ ضرب الـ Refractive Index
+
+132
+00:09:57,840 --> 00:10:04,340
+هو الـ Nمايبقاش هي θ هي عبارة عن زاوية الانكسار لو
+
+133
+00:10:04,340 --> 00:10:15,940
+أنا عندي هاي ال slide و مرضو عبر ال slide هاي
+
+134
+00:10:15,940 --> 00:10:23,200
+ال slide و مرضو عبر هاي ال slide الضوء لما يمر عبر
+
+135
+00:10:23,200 --> 00:10:29,870
+ال slide هينكسر بزاوية هاي الزاويةهي عبارة عن θ
+
+136
+00:10:29,870 --> 00:10:33,970
+«زاوية الانكسار» هي θ بعوض فيها في «unrefractive
+
+137
+00:10:33,970 --> 00:10:38,510
+index» ومعامل انكسار الوصف الموجود ما بين الـ
+
+138
+00:10:38,510 --> 00:10:41,870
+«slide» وما بين العدسة الشيئية اللي هنتكلم فيه
+
+139
+00:10:41,870 --> 00:10:45,950
+بآخر المعبر الـ numerical aperture بيقولك بيتمد
+
+140
+00:10:45,950 --> 00:10:50,770
+على الـ radius of lens بيتمد على نصف قطر العدسة
+
+141
+00:10:50,770 --> 00:10:54,510
+والعلاقة ما بين الـ numerical aperture وradius
+
+142
+00:10:54,510 --> 00:10:56,890
+علاقة عكسية، تمام؟
+
+143
+00:11:00,010 --> 00:11:07,370
+نرجع لـ slide الـ «high» «The larger numerical
+
+144
+00:11:07,370 --> 00:11:11,750
+aperture, the smaller the dissolvable distance and
+
+145
+00:11:11,750 --> 00:11:14,990
+hence the more efficient the resolution power»
+
+146
+00:11:17,070 --> 00:11:20,290
+محتاجة كمية ضوء اكتر كل ما زاد ال numerical
+
+147
+00:11:20,290 --> 00:11:24,830
+aperture كل ما قدرت اميز بين نقطتان المسافة بينهم
+
+148
+00:11:24,830 --> 00:11:27,870
+اكتر قليلة بيكون بالتالي بيكون ال resolution .. ال
+
+149
+00:11:27,870 --> 00:11:32,110
+resolution لأ ال microscope او العدسة اكتر عالي
+
+150
+00:11:32,110 --> 00:11:35,190
+فيعرفنا العلاقة الرياضية عشان نفهم العلاقة
+
+151
+00:11:35,190 --> 00:11:35,870
+الرياضية
+
+152
+00:11:48,610 --> 00:11:55,250
+هنا نكتبها مكتوب
+
+153
+00:11:55,250 --> 00:12:07,930
+في عندي R اللي هو R
+
+154
+00:12:07,930 --> 00:12:16,150
+بساوي ال .. في عندي رقم مكتوب
+
+155
+00:12:45,120 --> 00:12:48,500
+الرقم درب ال web links على ال numerical aperture
+
+156
+00:12:57,620 --> 00:13:04,060
+هذه هي العلاقة الرياضية خلّينا نحكي عن كل جزء بهذه
+
+157
+00:13:04,060 --> 00:13:08,680
+العلاقة R هي عبارة عن مقياس أصغر مسافة قابلة
+
+158
+00:13:08,680 --> 00:13:13,900
+للتمييز بين نقطتين R هي مقياس يعبر عن distance عن
+
+159
+00:13:13,900 --> 00:13:17,820
+مسافة أصغر مسافة قابلة للتمييز بين نقطتين طبعا هذا
+
+160
+00:13:17,820 --> 00:13:22,020
+ثابت ال wavelength هو الطول الموجي طبعا أحنا قلنا
+
+161
+00:13:22,020 --> 00:13:26,160
+أن الضوء يمر عبر العينةفأنا سوء طول الموج اللي هدق
+
+162
+00:13:26,160 --> 00:13:29,680
+الضوء اللي بمر عبر العينة بعود عنه في هذا القانون
+
+163
+00:13:29,680 --> 00:13:33,880
+الـ numerical aperture هي كمية الضوء اللي مار عبر
+
+164
+00:13:33,880 --> 00:13:38,580
+العينة طيب، ان هاي العلاقة أنا بستنتج ان في عندي
+
+165
+00:13:38,580 --> 00:13:43,020
+أو قبل ما نحكي عن هاي العلاقة هلأ احنا قولنا كل ما
+
+166
+00:13:43,020 --> 00:13:48,460
+زاد ال magnification كل ما احتجت كمية الضوء أكتر
+
+167
+00:13:48,460 --> 00:13:52,610
+كل ما زاد ال magnification كل ما احتجتالكمية الضوء
+
+168
+00:13:52,610 --> 00:13:57,350
+أكتر اللي هو الـ numerical aperture أكتر فبالتالي
+
+169
+00:13:57,350 --> 00:14:02,570
+كل ما كان الـ resolution لأ الميكروسكوب كتير عالي
+
+170
+00:14:02,570 --> 00:14:07,250
+فبالتالي اقدرت اميز بين نقطتين المسافة بينهم كتير
+
+171
+00:14:07,250 --> 00:14:12,430
+قليلة يبقى R هي المسافة اللي بين النقطتين كتير
+
+172
+00:14:12,430 --> 00:14:16,910
+قليلة اقدرت اميز بينهم وكان كمية الضوء عالية و ال
+
+173
+00:14:16,910 --> 00:14:20,010
+resolution عالية يبقى لو بنكمل القانون عشان تكون
+
+174
+00:14:20,010 --> 00:14:26,070
+الأمور واضحة أكتربنكتب أن الـ R هي مسافة بين
+
+175
+00:14:26,070 --> 00:14:35,550
+نقطتين وهي معكوث الـ resolution يبقى
+
+176
+00:14:35,550 --> 00:14:44,890
+الواحد
+
+177
+00:14:44,890 --> 00:14:50,750
+و الـ
+
+178
+00:14:50,750 --> 00:14:51,270
+resolution
+
+179
+00:15:21,890 --> 00:15:25,850
+يبقى كل ما زاد الـ Magnification كل ما احتجت كمية
+
+180
+00:15:25,850 --> 00:15:30,910
+الضوء أكتر فبالتالي كل ما كانت قوة الـ Resolution
+
+181
+00:15:30,910 --> 00:15:35,790
+دقة الميكروسكوب أو العدسة أكتر فمعناها إيش يعني
+
+182
+00:15:35,790 --> 00:15:39,110
+الـ Resolution يعني اقدر اتميز بين نقطتين المسافة
+
+183
+00:15:39,110 --> 00:15:44,250
+بينهم كثير قليلاً من هذه العلاقة نستنتج علاقة أخرى
+
+184
+00:15:44,250 --> 00:15:47,270
+إنه ما بين الـ Resolution وما بين طول الموج اللي
+
+185
+00:15:47,270 --> 00:15:56,720
+هي علاقة عكسيةكل ما كان الطول الموجي اكتير قليل كل
+
+186
+00:15:56,720 --> 00:16:01,840
+ما كان الطول الموجي لضوء الماء عبر العين القصير كل
+
+187
+00:16:01,840 --> 00:16:05,760
+ما كان الـ Resolution عالي وقدرت اميز بين نقطتان
+
+188
+00:16:05,760 --> 00:16:10,400
+المسافة بينهم اكتر قليلة تمام؟ هذا بالنسبة للعلاقة
+
+189
+00:16:10,400 --> 00:16:14,600
+الرياضية المضحة بتمنى تكون واضحة كل الأمور تمام
+
+190
+00:16:22,560 --> 00:16:26,220
+الـ «depth of focus» معرفة ليها «thickness of
+
+191
+00:16:26,220 --> 00:16:30,460
+field» «سمك المجال» اللي هو عمق المسافة أو عمق
+
+192
+00:16:30,460 --> 00:16:34,760
+الرؤية معرفة ليها «thickness of field» اللي هو سمك
+
+193
+00:16:34,760 --> 00:16:39,080
+المجال «as magnification increases depth of focus
+
+194
+00:16:39,080 --> 00:16:43,140
+increases» إيش يعني سمك المجال أو سمك الـ field؟
+
+195
+00:16:43,140 --> 00:16:46,400
+معناه لو كان أنا عندي كتير طبقات لو عندي عينة فيها
+
+196
+00:16:46,400 --> 00:16:50,400
+كتير طبقات أنا
+
+197
+00:16:50,400 --> 00:16:55,620
+ماعملتلها تقطيعكانت العينات تتكون من عدة طبقات
+
+198
+00:16:55,620 --> 00:17:01,080
+وعينة أخرى كانت تتكون من طبقتين كل ما كانت الطبقات
+
+199
+00:17:01,080 --> 00:17:04,960
+أقل في هذه الناحية كل ما كان يعني ال depth of
+
+200
+00:17:04,960 --> 00:17:09,210
+focus كتير قليلفبالتالي بيكون الـ Resolution وضوح
+
+201
+00:17:09,210 --> 00:17:12,470
+الصورة في المنطقة اللي بيكون عندها الطبقات الأقل
+
+202
+00:17:12,470 --> 00:17:16,710
+بتكون الـ Resolution عالية وبالتالي بتحتاج قوة
+
+203
+00:17:16,710 --> 00:17:20,870
+تكبير عالية يبقى كل ما كانت الطبقات أقل يعني الـ
+
+204
+00:17:20,870 --> 00:17:23,850
+Depth of Focus كان أليل كل ما كان الـ Resolution
+
+205
+00:17:23,850 --> 00:17:26,830
+أكيد لما تكون الطبقات أليلة هيكون وضوح الصورة أكتر
+
+206
+00:17:26,830 --> 00:17:29,890
+الـ Resolution أكتر فبالتالي الـ Magnification
+
+207
+00:17:29,890 --> 00:17:32,470
+بيزيد يبقى العلاقة ما بين الـ Depth of Focus
+
+208
+00:17:32,470 --> 00:17:37,350
+والمagnification علاقة عكسيةبالنسبة للـ Field of
+
+209
+00:17:37,350 --> 00:17:41,550
+Effusion الـ Field of Effusion هي الـ Surface Area
+
+210
+00:17:41,550 --> 00:17:45,950
+of View هي المساحة اللي أنا بدي أشوفها بقولك As a
+
+211
+00:17:45,950 --> 00:17:49,690
+magnification increase the area of view decrease
+
+212
+00:17:49,690 --> 00:17:54,370
+كل ما زادت قوتي التكبير كل ما قلت مساحة المنطقة
+
+213
+00:17:54,370 --> 00:17:59,170
+اللي أنا بدي أشوفها وهذا أشي طبيعي في 4X لو أنا
+
+214
+00:17:59,170 --> 00:18:03,770
+جبت ورقة شجر وهي في مرسومة موجودة في slide سابقة
+
+215
+00:18:06,880 --> 00:18:11,080
+بنسبة لـ 4x لو أنا جبت ورقة شجر و روحت حطيتها على
+
+216
+00:18:11,080 --> 00:18:15,520
+4x هشوف كل ورقة الشجر هتكون المساحة اللي انا بدي
+
+217
+00:18:15,520 --> 00:18:19,900
+اشوفها كتير كبيرة لكن لو انا زودت ال magnification
+
+218
+00:18:19,900 --> 00:18:23,460
+روحت على 10x بالتالي المساحة اللي انا شوفتها راحت
+
+219
+00:18:23,460 --> 00:18:27,740
+هذه الأزاق و شوفت فقط جزءأصغر من اللي أنا شوفته
+
+220
+00:18:27,740 --> 00:18:32,940
+فيه 4X وكذلك فيه بالنسبة لـ 40X كل ما زودت قوة
+
+221
+00:18:32,940 --> 00:18:35,700
+التكبير كل ما زاد الـ Magnification كل ما كانت
+
+222
+00:18:35,700 --> 00:18:38,420
+المساحة اللي أنا بدي أشوفها أقل راحت المساحة high
+
+223
+00:18:38,420 --> 00:18:42,600
+وشوفت فقط مساحة أقل شوفت high المساحة ونفس الفكرة
+
+224
+00:18:42,600 --> 00:18:47,000
+بالنسبة للـ Oil immersion هشوف فقط مساحة أقل هشوف
+
+225
+00:18:47,000 --> 00:18:51,420
+high المساحة فقط يبقى كانت علاقة عكسية بين الـ
+
+226
+00:18:51,420 --> 00:18:54,920
+Field of Fusion وما بين الـ Magnification
+
+227
+00:19:05,020 --> 00:19:08,720
+as a magnification increase the resolution also
+
+228
+00:19:08,720 --> 00:19:11,980
+increase اتفقنا كل ما زاد ال magnification كل ما
+
+229
+00:19:11,980 --> 00:19:14,940
+زاد ال resolution يعني اقدرت اميز بين نقطتين
+
+230
+00:19:14,940 --> 00:19:18,580
+المسافة بينهم كتير قليلة و لو بدنا نوضح اكتر هذه
+
+231
+00:19:18,580 --> 00:19:21,300
+النقطة لو انت كنت راكبة بسيارة و السيارة بتمشي
+
+232
+00:19:21,300 --> 00:19:25,880
+بسرعة معينة كان في ضوء أحمر من مسافة بعيدة كل ما
+
+233
+00:19:25,880 --> 00:19:29,620
+اقتربتي و كل ما اقدرتي ان اتميزي ان ضوء هذا عبارة
+
+234
+00:19:29,620 --> 00:19:35,240
+كان عن نصباحين فوق بعضوبالتالي قدرتي اتميزي هدول
+
+235
+00:19:35,240 --> 00:19:39,180
+الكائنين اللي كانت المسافة بينهم كتير قريبة انت كل
+
+236
+00:19:39,180 --> 00:19:43,040
+ما قربتي معناه بما يقابله في الميكروسكوب كل ما
+
+237
+00:19:43,040 --> 00:19:46,140
+كبرتوا كل ما زادت الـ Magnification كل ما قدرتي
+
+238
+00:19:46,140 --> 00:19:50,260
+انك اتميزي بين جسمان كائنين المسافة بينهم كتير
+
+239
+00:19:50,260 --> 00:19:53,840
+صغيرة to achieve a high magnification with good
+
+240
+00:19:53,840 --> 00:19:57,360
+resolution the objective lens must have a small
+
+241
+00:19:57,360 --> 00:20:02,250
+radius كلما وقولنا انه الـ numerical apertureالها
+
+242
+00:20:02,250 --> 00:20:06,510
+علاقة تعتمد على الـ «Radius of Length» وأنا قلت
+
+243
+00:20:06,510 --> 00:20:10,170
+إنه العلاقة عكسية نرجع شوية نرجع المعلومات كل ما
+
+244
+00:20:10,170 --> 00:20:13,090
+زاد الـ Magnification كل ما احتجت ميضه أكتر يعني
+
+245
+00:20:13,090 --> 00:20:16,490
+الـ numerical aperture كان عالي كل ما كان الـ
+
+246
+00:20:16,490 --> 00:20:19,750
+Resolution عالي طب أنا قلت ال numerical aperture
+
+247
+00:20:19,750 --> 00:20:23,810
+علاقته عكسية مع الـ Radius يبقى هيكون الـ Radius
+
+248
+00:20:23,810 --> 00:20:26,970
+علاقته عكسية مع كل من الـ Magnification ومع الـ
+
+249
+00:20:26,970 --> 00:20:30,430
+Resolutionيبقى وهذا اللي بيأكد هذا الكلام يبقى
+
+250
+00:20:30,430 --> 00:20:33,890
+Magnification هي لأ كل ما زاد لإن المرق الأبراطور
+
+251
+00:20:33,890 --> 00:20:37,390
+بيزيد ال Resolution بيزيد ويقل ال Radius بقالت
+
+252
+00:20:37,390 --> 00:20:42,450
+يبقى عدسة عشرة X ال Radius تبقى هيبقى أصغر من عدسة
+
+253
+00:20:42,450 --> 00:20:45,530
+أربعة X من هاي اللي اللي علاقة نستنفتش هذه
+
+254
+00:20:45,530 --> 00:20:50,410
+المعلومة shorter wavelength of light provide
+
+255
+00:20:50,410 --> 00:20:54,320
+greater resolutionاتفقنا حسب العلاقة اللي أخدناها
+
+256
+00:20:54,320 --> 00:20:57,000
+الـ «R» اللي هي انه قياس أصغر مسافة بين نقطتين
+
+257
+00:20:57,000 --> 00:21:01,920
+بتساوي 0.612 درب الـ «Wavelength» على الـ
+
+258
+00:21:01,920 --> 00:21:05,220
+«Numerical Aperture» إنه في عندي علاقة عكسية ما
+
+259
+00:21:05,220 --> 00:21:08,520
+بين الـ «Resolution» وما بين الـ «Wavelength» ما
+
+260
+00:21:08,520 --> 00:21:12,300
+بين الطول الموجي الطول الموجي هو .. الطول الموجي
+
+261
+00:21:12,300 --> 00:21:14,700
+هي خاصية في الضوء حسب شو أنا .. الضوء اللي أنا
+
+262
+00:21:14,700 --> 00:21:18,540
+استخدمته بستخدم الطول الموجي لهذا الضوء واللي
+
+263
+00:21:18,540 --> 00:21:22,930
+بيأكد هذا الكلام أنهالمشهر الإلكتروني أنا بستخدم
+
+264
+00:21:22,930 --> 00:21:27,370
+إلكترونات الطول الموجي للإلكترونات هي أقل من الطول
+
+265
+00:21:27,370 --> 00:21:30,970
+الموجي للضوء المريء عشان هيك ال resolution للمشهر
+
+266
+00:21:30,970 --> 00:21:34,890
+الإلكتروني بيكون أعلى من ال resolution للمشهر
+
+267
+00:21:34,890 --> 00:21:40,090
+الضوءي لأن ال wavelength للإلكترونات أقل من ال
+
+268
+00:21:40,090 --> 00:21:42,010
+wavelength للضوء المريء
+
+269
+00:21:45,220 --> 00:21:48,580
+هنعملّي شوية مراجعة على كل الإلاقات اللي اتكلمنا
+
+270
+00:21:48,580 --> 00:21:51,840
+عنها «depth of focus» الـ «depth of focus» قولنا
+
+271
+00:21:51,840 --> 00:21:56,240
+هو thickness of specimen هو ثمك المجال that can be
+
+272
+00:21:56,240 --> 00:22:00,120
+seen in focus at one time قولنا كل إلاقة عكسية ما
+
+273
+00:22:00,120 --> 00:22:02,520
+بين الـ magnification وما بين الـ depth of focus
+
+274
+00:22:02,520 --> 00:22:05,900
+الـ field of vision هو المساحة اللي أنا بدي أشوفها
+
+275
+00:22:05,900 --> 00:22:09,260
+قولنا إنه الإلاقة برضه عكسية يبقى الـ
+
+276
+00:22:09,260 --> 00:22:11,760
+magnification معقول من الـ depth of focus والـ
+
+277
+00:22:11,760 --> 00:22:15,610
+field of vision إلاقة عكسيةلكن الـ Magnification
+
+278
+00:22:15,610 --> 00:22:19,750
+مع الـ Resolution ومع الـ numerical aperture كلهم
+
+279
+00:22:19,750 --> 00:22:22,870
+علاقة ترضية كل ما زاد الـ Magnification كل ما
+
+280
+00:22:22,870 --> 00:22:25,730
+احتجت كمية الضوء اكتر تصل العينة بتتالي الـ
+
+281
+00:22:25,730 --> 00:22:29,050
+Resolution لزيها هاي ملخص كل العلاقات اللي اتكلمنا
+
+282
+00:22:29,050 --> 00:22:33,290
+فيها الـ Medium between the objective and the
+
+283
+00:22:33,290 --> 00:22:36,370
+object is a factor that must be taken into
+
+284
+00:22:36,370 --> 00:22:39,310
+consideration for the most effective use of
+
+285
+00:22:39,310 --> 00:22:44,110
+microscope lensبقولّك الوسط بين العدسة الشيئية وما
+
+286
+00:22:44,110 --> 00:22:48,410
+بين العينة لازم ناخدها بعين الاعتبار حتى إن هتكون
+
+287
+00:22:48,410 --> 00:22:52,030
+الصورة واضحة أنا قولت كل ما زاد الـ Magnification
+
+288
+00:22:52,030 --> 00:22:56,750
+كل ما زاد الـ Resolution لكن احنا قلنا لازم بعض
+
+289
+00:22:56,750 --> 00:23:00,930
+الأحيان أنه ناخد بعين الاعتبار أمور منها الـ Fine
+
+290
+00:23:00,930 --> 00:23:05,570
+Adjustment لما انا أروح من 4X أروح لـ40X لازم ألعب
+
+291
+00:23:05,570 --> 00:23:09,370
+بالـFine Adjustment لازم أتحكم بإداء فرق من كمية
+
+292
+00:23:09,370 --> 00:23:12,890
+الضوءواصلة لهذه العينة من الأمور المهمة اللي لازم
+
+293
+00:23:12,890 --> 00:23:18,130
+أخدها بعين الاعتبار منها برضه الوسط ما بين العدسة
+
+294
+00:23:18,130 --> 00:23:22,030
+الشيئية وما بين الـ Slots بقولك The Lower Power
+
+295
+00:23:22,030 --> 00:23:27,150
+Objective 4X 10X and High Dry Objective 40X Loose
+
+296
+00:23:27,150 --> 00:23:31,910
+Air احنا قلنا ان الـ 4X اسمها Scanning Objective
+
+297
+00:23:31,910 --> 00:23:36,560
+لكن هنا هو مسميها Low Powerلكن إحنا خلّينا نتفق
+
+298
+00:23:36,560 --> 00:23:41,080
+إنه الـ 4X هي تعتبر Scanning الـ 10X تعتبر Law
+
+299
+00:23:41,080 --> 00:23:45,000
+Power لكن لو أنا سميت الـ 4X Law Power عادي هي
+
+300
+00:23:45,000 --> 00:23:48,680
+Scanning أو Law Power ولكن الـ High Power هي الـ
+
+301
+00:23:48,680 --> 00:23:53,180
+40X أقولك في العدسة الشيئية 4 وفي العدسة الشيئية
+
+302
+00:23:53,180 --> 00:23:57,700
+10X و40X باستخدام يكون الوسط ما بين العدسة الشيئية
+
+303
+00:23:57,700 --> 00:24:02,020
+وما بين الـ Slide يكون الهواء لكن عند العدسة 100
+
+304
+00:24:02,930 --> 00:24:05,930
+الـ Oil Emergent بستخدم الـ Oil عشان هي احنا
+
+305
+00:24:05,930 --> 00:24:10,090
+بنسميها Oil Emergent لأنه بنضيف الـ Oil طيب ليش
+
+306
+00:24:10,090 --> 00:24:15,410
+أنا بضيف Oil عند 100X؟ بقولك لأن الـ Z يلو نفس
+
+307
+00:24:15,410 --> 00:24:20,690
+معامل الزجاج حلو لما ينتقل الضوء مابان وسطين معامل
+
+308
+00:24:20,690 --> 00:24:24,870
+انكسار هو مختلف بتشطط الضوء بتشطط الضوء يعني ال
+
+309
+00:24:24,870 --> 00:24:28,830
+numerical aperture يعني كمية الضوء الواصلة للعينة
+
+310
+00:24:28,830 --> 00:24:32,900
+بتكون قليلةبالتالي الـ Resolution هيقل وضوح الصورة
+
+311
+00:24:32,900 --> 00:24:36,640
+هيقل، عشان هيك أنا بستخدم الـ Oil اللي له نفس
+
+312
+00:24:36,640 --> 00:24:41,200
+معامل الزجاج، وبالتالي مش هيتشطط الضوء، هيتجمع على
+
+313
+00:24:41,200 --> 00:24:45,640
+العدسة، هيوصل كمية كبيرة الضوء لعينة، numerical
+
+314
+00:24:45,640 --> 00:24:48,700
+aperture هيكون عالي، بالتالي هيكون الـ Resolution
+
+315
+00:24:48,700 --> 00:24:54,500
+وضوح الصورة أكتر في حالة أن أنا لو ضفت Oilتمام،
+
+316
+00:24:54,500 --> 00:24:57,540
+هذا بالنسبة للسبب إن أنا بحط Oil ‏when oil
+
+317
+00:24:57,540 --> 00:25:00,400
+emergence lens are employed ‏a drop of oil should
+
+318
+00:25:00,400 --> 00:25:02,820
+be used ‏أقول لك، لو أنا استخدمت الـ Oil emergence
+
+319
+00:25:02,820 --> 00:25:07,780
+lens ‏اللي احنا سميناها هي الـ 100X Objective
+
+320
+00:25:07,780 --> 00:25:12,740
+‏بقولك، نضيف نقطة من إزاي ‏وليه هذا السبب؟
+
+321
+00:25:12,740 --> 00:25:15,520
+‏binding of the light waves and cursants ‏لأن
+
+322
+00:25:15,520 --> 00:25:20,830
+بيصير في عندي انحناء للضوءOil has the same
+
+323
+00:25:20,830 --> 00:25:24,310
+refractive index of the glass while air in the
+
+324
+00:25:24,310 --> 00:25:28,030
+case of refraction لأن معامل انكسار ذات إنه نفس
+
+325
+00:25:28,030 --> 00:25:32,330
+معامل انكسار الزرجاج لكن بالنسبة للهواء معامل
+
+326
+00:25:32,330 --> 00:25:37,190
+انكسار الهواء أقل من الزرجاج وهذا بيقدّي إنه
+
+327
+00:25:37,190 --> 00:25:41,690
+يتشدّر الضوء أكتر أتقل كمية الضوء الواصلة للعينة
+
+328
+00:25:41,690 --> 00:25:43,450
+بالتالي الـ Resolution تقل
+
+329
+00:25:47,540 --> 00:25:52,600
+هنحملك صورة مقارنة بين لو أنا بدون ما أحط oil على
+
+330
+00:25:52,600 --> 00:25:57,580
+100X و لو أنا حطيت oil على العدسة 100 لو أنا ما
+
+331
+00:25:57,580 --> 00:26:01,920
+حطيت oil على العدسة 100 بقولك إنه لما يمر الضوء،
+
+332
+00:26:01,920 --> 00:26:07,100
+الضوء هيتشتت فبالتالي مش هيوصل كمية كبيرة للعينة
+
+333
+00:26:07,100 --> 00:26:11,700
+مش هيوصل كمية كبيرة من الضوء للعينة أو للعدسة
+
+334
+00:26:11,700 --> 00:26:15,300
+الشيئية فبالتالي الـ Resolution ألف لكن لو أنا
+
+335
+00:26:15,300 --> 00:26:19,380
+استخدمت الـ Oilبالتالي الضوء مش هيتشتت هيتجمع على
+
+336
+00:26:19,380 --> 00:26:24,000
+العدس الشيئية هيوصل كمية الضوء كبيرة فبالتالي ال
+
+337
+00:26:24,000 --> 00:26:29,140
+resolution وضوح الصورة بزيه Refractive إيش يعني؟
+
+338
+00:26:29,140 --> 00:26:32,420
+Refractive يعني bending إنكسار أو إنحناء
+
+339
+00:26:32,420 --> 00:26:35,240
+Refractive index is a measurement of the extent
+
+340
+00:26:35,240 --> 00:26:39,440
+that a substance bends light هو بيقولك بيقيس معدل
+
+341
+00:26:39,440 --> 00:26:45,220
+إنكسار الضوءfield of view or equal لو كان اللي هو
+
+342
+00:26:45,220 --> 00:26:48,060
+الـ total magnification field of view يعني الـ
+
+343
+00:26:48,060 --> 00:26:51,140
+total magnification بقولك لو كان أكبر أو يساوي
+
+344
+00:26:51,140 --> 00:26:56,000
+500x cause increase in diffraction and this cause
+
+345
+00:26:56,000 --> 00:26:59,300
+distortion of the image مش معناه، هلأ أنا لما
+
+346
+00:26:59,300 --> 00:27:02,960
+أستخدم 4x وقولنا معامل الإنكسار أو total sorry
+
+347
+00:27:02,960 --> 00:27:07,700
+total magnification بكون 40 لو أنا استخدمت 10x
+
+348
+00:27:07,700 --> 00:27:12,710
+بكون اللي هي100 Total Magnification لو أنا استخدمت
+
+349
+00:27:12,710 --> 00:27:18,210
+40X الـ Total Magnification بيكون 400X طيب لو أنا
+
+350
+00:27:18,210 --> 00:27:22,050
+استخدمت 100 بيكون الـ Total Magnification 1000X
+
+351
+00:27:22,050 --> 00:27:26,190
+وين الـ Total Magnification بيكون أعلى من 500 لو
+
+352
+00:27:26,190 --> 00:27:30,130
+أنا استخدمت اللي هي الـ 100X Objective لو أنا
+
+353
+00:27:30,130 --> 00:27:33,750
+استخدمت عدسة العدسة الشيئية 100 هيكون الـ Total
+
+354
+00:27:33,750 --> 00:27:38,440
+Magnification أكتر من 500فبالتالي في العدسة
+
+355
+00:27:38,440 --> 00:27:43,060
+الشيئية 100X بيقولّك بيزيد لما يكون الـ Total
+
+356
+00:27:43,060 --> 00:27:46,640
+Magnification أكتر من 500 اللي هو عن 100X بيزيد
+
+357
+00:27:46,640 --> 00:27:51,360
+تشطط الضوء فبالتالي عشان هيك أنا بوضع .. بحط oil
+
+358
+00:27:51,360 --> 00:27:54,180
+عند الـ 100X لأن الـ Total Magnification اللي
+
+359
+00:27:54,180 --> 00:27:58,340
+نبعها أكتر من 500 بالتالي هيكون عند الـ numerical
+
+360
+00:27:58,340 --> 00:28:01,340
+aperture هيتشطط الضوء ال numerical aperture هيكون
+
+361
+00:28:01,340 --> 00:28:05,490
+قليل ال-resolution قليل بالتاليقلتها تسبب
+
+362
+00:28:05,490 --> 00:28:08,430
+Distortion of the image ‏هيكون فيها اندى قلة في
+
+363
+00:28:08,430 --> 00:28:11,710
+وضوح الصورة ‏وقلنا ليش لأنه اتشتت اللوء ما تجمع
+
+364
+00:28:11,710 --> 00:28:15,350
+على العينة ‏Caline numerical aperture ألي ‏هذا
+
+365
+00:28:15,350 --> 00:28:19,210
+بالنسبة للعلاقات
+
+366
+00:28:19,210 --> 00:28:23,410
+المهمة ‏بالنسبة لل light compound microscopeهلأ
+
+367
+00:28:23,410 --> 00:28:26,650
+بقولك كيف أنا أستخدم الـMicroscope أول شيء طبعا
+
+368
+00:28:26,650 --> 00:28:32,570
+بحط الفيشة في الكهربا بضوي مصدر الإضاءة و بحط على
+
+369
+00:28:32,570 --> 00:28:37,490
+أربعة ببدأ أنا بالـLow Power و بعدين بنتقل لـHigh
+
+370
+00:28:37,490 --> 00:28:42,190
+Power بحط على 4X Objective بحط الـSmear على
+
+371
+00:28:42,190 --> 00:28:44,590
+الـStage الـSmear يعني أني أنا بحط الـSlide أو
+
+372
+00:28:44,590 --> 00:28:48,030
+العين اللي أنا بديها على الـStage و بشوفها على
+
+373
+00:28:48,030 --> 00:28:52,870
+الـ4X بستخدم الـCourse Adjustment Logأحنا قلنا إن
+
+374
+00:28:52,870 --> 00:28:55,970
+ال course adjustment knob حتى إن أنا أستخدمه حتى
+
+375
+00:28:55,970 --> 00:29:01,170
+إن أنا أجيب ال fail فبحركه طبعا ممكن إن أنا أحط ال
+
+376
+00:29:01,170 --> 00:29:06,010
+stage أخليها للآخر اللي فوق و أبدأ إن أنا أنزل أو
+
+377
+00:29:06,010 --> 00:29:09,530
+إن أنا بنزلها للآخر و ببدأ بتطلع شوية شوية حتى إن
+
+378
+00:29:09,530 --> 00:29:12,630
+أنا أشوف الصورة اللي أنا بديها هيك معناه إن أنا
+
+379
+00:29:12,630 --> 00:29:16,650
+جبت ال failبقولك بعدين بتنتقلي للـ «objective
+
+380
+00:29:16,650 --> 00:29:20,690
+lens» التانية العشرة X وما بعدها اللي هي الأربعين
+
+381
+00:29:20,690 --> 00:29:25,530
+وأخر شئ بتوصلي طبعا لما توصلي لأربعين بتحاول أنك
+
+382
+00:29:25,530 --> 00:29:28,650
+تحركي الـFine Adjustment حتى أنه تكون
+
+383
+00:29:28,650 --> 00:29:32,510
+الـResolution للـImage واضحة لما تستخدمي الـOil
+
+384
+00:29:32,510 --> 00:29:35,930
+Immersion بقولك بتحطي Drop of Oil واتفقنا ليش
+
+385
+00:29:35,930 --> 00:29:39,890
+بإننا بنحط Oil على الـ100X كافي بتمسكي
+
+386
+00:29:39,890 --> 00:29:43,740
+الـMicroscope بقولك بتمسكيهو إيد بتمسك الـ ARM
+
+387
+00:29:43,740 --> 00:29:46,760
+وإيد تانية بتمسك الـ Bezel زي ما إحنا شايفين أنه
+
+388
+00:29:46,760 --> 00:29:50,440
+هان إيد ماسكة الـ Bezel قاعدة والإيد التانية ماسكة
+
+389
+00:29:50,440 --> 00:29:55,360
+الـ ARM في بعض العوامل اللي بتأثر على جودة الصورة
+
+390
+00:29:55,360 --> 00:29:58,860
+Quality of Microscope الـ Antlens اتكلمنا عن هذا
+
+391
+00:29:58,860 --> 00:30:03,460
+الموضوع أنه جودة الـ Microscope و العدسات طبعا
+
+392
+00:30:03,460 --> 00:30:07,700
+مثلا على سبيل المثال الـ Microscope الإلكتروني الـ
+
+393
+00:30:07,700 --> 00:30:10,560
+Resolution تبعه أكيد هيكون أعلى من الـ Microscope
+
+394
+00:30:10,560 --> 00:30:13,720
+الضوءيالـ Size of sample، الـ type of sample أو
+
+395
+00:30:13,720 --> 00:30:16,700
+mouth of light on the sample كمية الضوء الواصلة
+
+396
+00:30:16,700 --> 00:30:22,240
+للعينة وجودة العينة كل هذه الأمور بتأثر على جودة
+
+397
+00:30:22,240 --> 00:30:26,020
+الصورة Microscope Maintenance كيف أنا بدي أحافظ
+
+398
+00:30:26,020 --> 00:30:31,420
+على الـ Microscope بقولك ضروري أني أنا أحافظ أني
+
+399
+00:30:31,420 --> 00:30:34,680
+أنا أخزنه بالطريقة الصحية هاي هتمنع كتير من
+
+400
+00:30:34,680 --> 00:30:38,820
+المشاكلهحميه من الـ Dust and dirt بدي أحميه من
+
+401
+00:30:38,820 --> 00:30:42,360
+الغبار و من الفندس و من الفطريات ا start the
+
+402
+00:30:42,360 --> 00:30:44,880
+microscope under a protection cover في عندي
+
+403
+00:30:44,880 --> 00:30:47,780
+protection cover كيس خاص فيه ال microscope بعد ما
+
+404
+00:30:47,780 --> 00:30:51,880
+أخلص و أنظف ال microscope بحطه فيه ال protection
+
+405
+00:30:51,880 --> 00:30:57,500
+cover و بحطه في ظروف تكون الرطوبة فيها قليلة كيف
+
+406
+00:30:57,500 --> 00:31:01,080
+أنا بدي أنظف ال microscope بقولك بستخدم لأ ال body
+
+407
+00:31:01,080 --> 00:31:05,820
+و للا ال stage اللي هي صبونةأما بالنسبة لأ العدسات
+
+408
+00:31:05,820 --> 00:31:08,060
+حتى أني أنا مضت في العدسات باستخدام الـ Ether
+
+409
+00:31:08,060 --> 00:31:11,280
+Alcohol أو الـ Alcohol أو في عندي Lens Cleaner
+
+410
+00:31:11,280 --> 00:31:17,960
+Solution Cleaner سائل خاص بالعدسات للتنظيف بالنسبة
+
+411
+00:31:17,960 --> 00:31:23,960
+لأدوات الـ Lens Paper بقولك في عندي بعض الـ Lens
+
+412
+00:31:23,960 --> 00:31:27,060
+Paper اللي هو Commercial Lens Tissue For Optics
+
+413
+00:31:27,060 --> 00:31:32,750
+بتكون ورق تجاري خاص بتنظيف العدسات بقولكاحذري أنك
+
+414
+00:31:32,750 --> 00:31:36,310
+انت تستخدمي rough paper protect انه يكون اللي هو
+
+415
+00:31:36,310 --> 00:31:40,710
+الورق يكون خشن لأنه هيك هيأذي العدسات ممكن بعض
+
+416
+00:31:40,710 --> 00:31:45,170
+الأمور التانية اللي انك تستبدليها ب fine tissue
+
+417
+00:31:45,170 --> 00:31:49,670
+paper اللي هو الكهنس الناعم مثلا ال cloth اللي هو
+
+418
+00:31:49,670 --> 00:31:53,470
+الشاس سلك اللي هو الحريق ممكن تستخدمي هدول كلهم في
+
+419
+00:31:53,470 --> 00:31:57,910
+تنظيف العدساتكيف انا بدي انظف من الـ IBs الـ
+
+420
+00:31:57,910 --> 00:32:01,810
+Objective الـ Microscope Stage الـ Microscope Body
+
+421
+00:32:01,810 --> 00:32:05,970
+الـ Condenser بقولك بالنسبة للـ IBs انت بتنظفيهم
+
+422
+00:32:05,970 --> 00:32:09,970
+بحركة دائرية Circular مواشن من الداخل للخارج او
+
+423
+00:32:09,970 --> 00:32:15,850
+زيجزاجي وبعدين طبعا بتنشفيهم بالنسبة لل IBs هي بعد
+
+424
+00:32:15,850 --> 00:32:19,430
+ما انت اتخلص بتعملي ال drying اذا انت احتجتي مرة
+
+425
+00:32:19,430 --> 00:32:23,820
+اخرى لتنظيف مطفيها و تعملي ال drying كمان اخرىالـ
+
+426
+00:32:23,820 --> 00:32:27,140
+Objective lens ممنوع انك انت تشيليها من الـ
+
+427
+00:32:27,140 --> 00:32:29,420
+Nosepiece الـ Nosepiece تكلمنا عنه القرص اللي هو
+
+428
+00:32:29,420 --> 00:32:33,520
+اللي بيحرك العدسات ممنوع تشيلي العدسات الشيئية من
+
+429
+00:32:33,520 --> 00:32:38,140
+الـ Nosepiece اخر شئ لو انت بدك تنطفي ال body
+
+430
+00:32:38,140 --> 00:32:42,100
+بقولك لازم ترفع الفيشة من الكهربا و بعدين تنطفي ال
+
+431
+00:32:42,100 --> 00:32:48,510
+body حق بيقولنا بصابونةحتى إنك تزيل كل القادورات
+
+432
+00:32:48,510 --> 00:32:51,950
+عنها أخر الشيء الـ Condenser أكيد لما قبل ما تنطفي
+
+433
+00:32:51,950 --> 00:32:55,530
+بتدخل فيه شغلناه بتنطفي الـ Condenser بـ Lentifree
+
+434
+00:32:55,530 --> 00:32:59,710
+Cotton الـ Swab و بتستخدمي الـ Lens Cleaning
+
+435
+00:32:59,710 --> 00:33:05,690
+Solution و أخر الشيء بتعملي .. بتمسحيه بـ Dry Swab
+
+436
+00:33:05,690 --> 00:33:10,030
+فهيك بنكون خلصنا معمل الـ Microscope ان شاء الله
+
+437
+00:33:10,030 --> 00:33:14,040
+تكون كل المعلومات واضحة بالنسبة لكمهيكون فينا
+
+438
+00:33:14,040 --> 00:33:18,320
+مناقشة الأسبوع اللي جاي يوم التلاتة الساعة أحد عشر
+
+439
+00:33:18,320 --> 00:33:22,500
+هيكون تكون المناقشة في معمل الـ Microscope ومعمل
+
+440
+00:33:22,500 --> 00:33:25,840
+الـ Introduction إذا في أي معلومات إن شاء الله
+
+441
+00:33:25,840 --> 00:33:29,800
+هنقشها في هذا اللقاء السلام عليكم ورحمة الله
+
+442
+00:33:29,800 --> 00:33:30,440
+وبركاته
+
diff --git a/PL9fwy3NUQKwZWgAb_V1ATlRRsH-6i3cPC/bxthOW-TT6Y_postprocess.srt b/PL9fwy3NUQKwZWgAb_V1ATlRRsH-6i3cPC/bxthOW-TT6Y_postprocess.srt
new file mode 100644
index 0000000000000000000000000000000000000000..e5c332fb7a76b3137c037c8bf6423a95c9f81e19
--- /dev/null
+++ b/PL9fwy3NUQKwZWgAb_V1ATlRRsH-6i3cPC/bxthOW-TT6Y_postprocess.srt
@@ -0,0 +1,2532 @@
+1
+00:00:02,680 --> 00:00:06,220
+السلام عليكم ورحمة الله وبركاته نستكمل معاكم
+
+2
+00:00:06,220 --> 00:00:09,560
+محاضرات مساق الـ Microbiology العملية في هذا
+
+3
+00:00:09,560 --> 00:00:12,720
+اللقاء هنتكلم عن Media Preparation and
+
+4
+00:00:12,720 --> 00:00:16,060
+Sterilization Aseptic Techniques Pure Culture
+
+5
+00:00:16,060 --> 00:00:20,220
+Concept هنتعرف على طريق تحضير الـ Media لـ
+
+6
+00:00:20,220 --> 00:00:23,420
+Sterilization التعقيم كيف بدي أعقم الأدوات اللي
+
+7
+00:00:23,420 --> 00:00:26,940
+بستخدمها في معمل الـ Microbiology طبعا الـ
+
+8
+00:00:26,940 --> 00:00:29,920
+Sterilization يعتبر من الـ Aseptic Technique الـ
+
+9
+00:00:29,920 --> 00:00:33,570
+Aseptic Technique هي كل الإجراءاتاللي أنا بتبيعها
+
+10
+00:00:33,570 --> 00:00:37,690
+حتى أمنع ال contamination ال pure culture هي
+
+11
+00:00:37,690 --> 00:00:42,410
+المزرعة اللي بتكون المستعمرات الكولونية أصلها خلية
+
+12
+00:00:42,410 --> 00:00:46,210
+بكتيرية واحدة يعني بلاحظ على الطبق أن المستعمرة
+
+13
+00:00:46,210 --> 00:00:52,050
+كلها نفس ال morphology هذه المحاضرة هنتعرف على كل
+
+14
+00:00:52,050 --> 00:00:53,950
+من هذه المواضيع بالتفصيل
+
+15
+00:00:59,080 --> 00:01:02,480
+الـ Natural Environment Microorganism usually
+
+16
+00:01:02,480 --> 00:01:06,720
+exists as Mixed Population إيش يعني Mixed
+
+17
+00:01:06,720 --> 00:01:10,860
+Population لو أنا جبت السواب طبعا السواب بستخدمها
+
+18
+00:01:10,860 --> 00:01:15,580
+لأخذ مسحات أخدت بالسواب مسحة من الطاولة و زرعت
+
+19
+00:01:15,580 --> 00:01:19,280
+عالمي على وسط غذائي بعدين حطيت الطبق في ال
+
+20
+00:01:19,280 --> 00:01:23,700
+incubator لمدة 24 ساعة يعني عملت incubation ال
+
+21
+00:01:23,700 --> 00:01:26,960
+incubator طبعا هيوصل الظروف المناسبة لنمو
+
+22
+00:01:26,960 --> 00:01:32,030
+البكتيريابعد 24 ساعة هلاقي باندي فيه نمو على الطبق
+
+23
+00:01:32,030 --> 00:01:36,570
+هلاقي كولوني هذه الكولوني هتكون مختلفة في ال
+
+24
+00:01:36,570 --> 00:01:41,170
+morphology يعني هلاقي مجموعة من ال micro organism
+
+25
+00:01:41,170 --> 00:01:47,390
+mixed microorganism او mixed population وليس كائن
+
+26
+00:01:47,390 --> 00:01:50,770
+واحد however we are
+
+27
+00:01:58,510 --> 00:02:03,410
+عشان أدرس خصائص هذه الكائنات و أتعرف عليها لازم
+
+28
+00:02:03,410 --> 00:02:08,930
+أاخد كل كائن لحال لازم يكون عندي a pure culture شو
+
+29
+00:02:08,930 --> 00:02:13,270
+يعني a pure culture؟ a pure culture is one in
+
+30
+00:02:13,270 --> 00:02:17,430
+which all organisms are descendants of the same
+
+31
+00:02:17,430 --> 00:02:21,840
+organismهي المزرعة اللي بتكون المستعمرات عليها من
+
+32
+00:02:21,840 --> 00:02:27,540
+نفس السلالة يعني أصلها كائن واحد يعني أصلها خلية
+
+33
+00:02:27,540 --> 00:02:32,260
+بسكيرية واحدة technique for obtaining pure culture
+
+34
+00:02:32,260 --> 00:02:36,380
+from a mixed population will be described in this
+
+35
+00:02:36,380 --> 00:02:40,980
+slide لو أنا إجبتني عينيا natural وروحت زراعتها
+
+36
+00:02:40,980 --> 00:02:45,960
+بعد 24 ساعة لحظة عندي مستعمرات حجمها كبير
+
+37
+00:02:45,960 --> 00:02:49,610
+ومستعمرات حجمها صغيرةيبقى هان عندي mixed
+
+38
+00:02:49,610 --> 00:02:54,610
+population عشان أعمل identification لكل واحدة لازم
+
+39
+00:02:54,610 --> 00:02:59,310
+أعمل pure culture هذا المعمل هنتعرف كيف أنا بدي
+
+40
+00:02:59,310 --> 00:03:03,530
+أعمل pure culture عشان أتعرف على كل بكتيريا علاحي
+
+41
+00:03:03,530 --> 00:03:07,510
+ده من ال mixed population اللي أنا زرعتها في الأول
+
+42
+00:03:13,340 --> 00:03:17,040
+In working with microorganism, we must have a
+
+43
+00:03:17,040 --> 00:03:20,980
+method of transferring a growing organism from a
+
+44
+00:03:20,980 --> 00:03:23,680
+pure culture to a sterile medium without
+
+45
+00:03:23,680 --> 00:03:28,460
+introducing any unwanted outside contaminants خلال
+
+46
+00:03:28,460 --> 00:03:32,160
+شغل في معمل الـ Microbiology لازم يكون في عندي طرق
+
+47
+00:03:32,160 --> 00:03:36,480
+و إجراءات لمنع ال contamination في حال نقلت ال
+
+48
+00:03:36,480 --> 00:03:41,040
+micro organism من pure culture ل sterile media
+
+49
+00:03:41,040 --> 00:03:45,220
+يعني لوحة تانيةلو كان في عندي pure culture على
+
+50
+00:03:45,220 --> 00:03:50,360
+سبيل المثال E.coli وبدي أزرعها على وصف ثاني لازم
+
+51
+00:03:50,360 --> 00:03:53,540
+ألتزم بكل الطرق والإجراءات لمنع ال contamination
+
+52
+00:03:53,540 --> 00:03:59,000
+خلال عملية النقل أو حتى لما بدي أنقل micro
+
+53
+00:03:59,000 --> 00:04:02,780
+organism من mixed population ل pure culture لازم
+
+54
+00:04:02,780 --> 00:04:06,880
+ألتزم بالإجراءات لمنع ال contamination من ضمن هذه
+
+55
+00:04:06,880 --> 00:04:09,640
+الإجراءات أني أنا أمسح ال bench بالكخول قبل ما
+
+56
+00:04:09,640 --> 00:04:14,500
+أبدأ الشغللو استخدمت نيكروم لوبي تم تعقيمه قبل ما
+
+57
+00:04:14,500 --> 00:04:19,280
+أنا أستخدمه أفتغل جانب اللهب كمان كل أدواتي اللي
+
+58
+00:04:19,280 --> 00:04:24,360
+بستخدمها تكون معقمة من لوب Media4Cep ألشاب ونتعرف
+
+59
+00:04:24,360 --> 00:04:28,160
+في هذه المحاضرة على طرق تعقيم كل أداة في معمل الـ
+
+60
+00:04:28,160 --> 00:04:32,460
+Microbiology this method of preventing unwanted
+
+61
+00:04:32,460 --> 00:04:36,820
+microorganismبقولك إن هاي الإجراءات اللي باطمن
+
+62
+00:04:36,820 --> 00:04:40,540
+فيها إن الوصف ما يصير فيه تلوث ما يصير كونتا
+
+63
+00:04:40,540 --> 00:04:44,300
+ماناشا بسميها الـ Aseptic technique يبقى الـ
+
+64
+00:04:44,300 --> 00:04:47,920
+Aseptic technique هي كل الإجراءات اللي بيتم
+
+65
+00:04:47,920 --> 00:04:56,540
+اتخاذها لمنع الكونتا ماناشا for
+
+66
+00:04:56,540 --> 00:05:00,420
+the most part بكتيريا وفيزيولوجياonly can be
+
+67
+00:05:00,420 --> 00:05:04,400
+studied in pure culture عشان أدرس حصائص البكتيريا
+
+68
+00:05:04,400 --> 00:05:08,940
+لازم يكون عندي pure culture مش mixed population
+
+69
+00:05:08,940 --> 00:05:13,140
+the best way to obtain a pure culture is to start
+
+70
+00:05:13,140 --> 00:05:17,380
+with a single bacterial cell عشان أحصل على pure
+
+71
+00:05:17,380 --> 00:05:22,450
+culture لازم أبدأ ب single bacterial cellماذا يعني
+
+72
+00:05:22,450 --> 00:05:25,910
+single bacterial cell يعني isolated colony لأن ال
+
+73
+00:05:25,910 --> 00:05:30,470
+isolated colony أصلها خلية بكتيرية واحدة فلما بدي
+
+74
+00:05:30,470 --> 00:05:33,870
+أعمل pure culture من mixed population بروح بزرع
+
+75
+00:05:33,870 --> 00:05:37,990
+بطريقة الأربع باخد ال isolated colony من الربع
+
+76
+00:05:37,990 --> 00:05:43,470
+الرابع هيك بضمن أن ال culture يكون pure لأن أصلها
+
+77
+00:05:43,470 --> 00:05:46,650
+ال isolated colony خلية بكتيرية واحدة
+
+78
+00:05:52,950 --> 00:05:57,970
+الخلية تنقسم بسرعة تنتج ملايين من الخلايا خلال 24
+
+79
+00:05:57,970 --> 00:06:03,550
+ساعة يبقى بعد 24 ساعة هلاقي عندي ملايين من الخلايا
+
+80
+00:06:03,550 --> 00:06:08,270
+البكتيرية طب لو أنا ما اتبعت الـ Aseptic Technique
+
+81
+00:06:08,270 --> 00:06:12,810
+وكان عندي Contaminant unwanted غير اللي أنا بدي
+
+82
+00:06:12,810 --> 00:06:17,490
+إيها هينمو الـ unwanted الـcontaminantوينقسم ويصير
+
+83
+00:06:17,490 --> 00:06:21,750
+فيها اندي كون تماناش يبقى ال culture هيصير mixed
+
+84
+00:06:21,750 --> 00:06:26,690
+population مش pure culture a single unwanted
+
+85
+00:06:26,690 --> 00:06:31,170
+continent so can do the same thing in a otherwise
+
+86
+00:06:31,170 --> 00:06:36,050
+pure culture making the culture useless يبقى صار
+
+87
+00:06:36,050 --> 00:06:39,470
+اندي mixed population يبقى ال culture بتصير
+
+88
+00:06:39,470 --> 00:06:44,750
+useless غير صالحة للاستخدام for this reason
+
+89
+00:06:44,750 --> 00:06:49,610
+اعتبرتكI guess the restricted trial procedure must
+
+90
+00:06:49,610 --> 00:06:53,410
+be used عشان أعمل identification لازم يكون عندي
+
+91
+00:06:53,410 --> 00:06:57,750
+pure culture وليس mixed population عشان هيك لازم
+
+92
+00:06:57,750 --> 00:07:02,030
+يكون فيه إجراءات صارمة عشان أمنع ال contamination
+
+93
+00:07:02,030 --> 00:07:06,650
+عشان
+
+94
+00:07:06,650 --> 00:07:10,870
+أزرع و أنمي ال micro organism لازم يكون عندي وسط
+
+95
+00:07:10,870 --> 00:07:16,630
+غذائي لنمو البكتيريابسمي هذا الوسط ال media ال
+
+96
+00:07:16,630 --> 00:07:20,870
+media هي الوسط الغذائي لنمو البكتيريا in working
+
+97
+00:07:20,870 --> 00:07:24,810
+with microorganism we must also have a sterile
+
+98
+00:07:24,810 --> 00:07:28,410
+nutrient containing media in which to grow the
+
+99
+00:07:28,410 --> 00:07:32,530
+organism طبعا ال media لازم تكون استيرال لازم تكون
+
+100
+00:07:32,530 --> 00:07:37,370
+معقمة وهذا من ال aseptic technique anything in or
+
+101
+00:07:37,370 --> 00:07:41,490
+on which we grow a microorganism is termeda medium
+
+102
+00:07:41,490 --> 00:07:45,670
+بقولي أي إشي بينمي ال micro organism بسميه medium
+
+103
+00:07:45,670 --> 00:07:50,890
+ال medium مفرد جمحا media a nutrient blend ال
+
+104
+00:07:50,890 --> 00:07:54,790
+blend يعني خليط used to support microbial growth
+
+105
+00:07:54,790 --> 00:07:59,670
+هي خليط من المواد الغذائية لنمو ال micro organism
+
+106
+00:07:59,670 --> 00:08:04,910
+dehydrate powder according to manufactural
+
+107
+00:08:04,910 --> 00:08:10,390
+instruction بقوليهذه الميديا بتكون باودر كيف انا
+
+108
+00:08:10,390 --> 00:08:15,630
+بدي احضرها بحلها بدستل واتر dehydrate يعني الباودر
+
+109
+00:08:15,630 --> 00:08:20,290
+بدي احلها بدستل واتر حسب التعليمات الموجودة على
+
+110
+00:08:20,290 --> 00:08:25,290
+البوتل على سبيل المثال New Zealand Agar بتيجي بوتل
+
+111
+00:08:25,290 --> 00:08:32,570
+مكتوب عليها كل 28 جرام بحلها بـ 1 لتر دستل واتر
+
+112
+00:08:32,570 --> 00:08:36,930
+طبعا حسب الكمية اللي انا بحضرها لو بدي احضر 500 مل
+
+113
+00:08:37,200 --> 00:08:41,800
+بحسب أكم من جرام بدّي من البودر وبحلها بـ 500 ملي
+
+114
+00:08:41,800 --> 00:08:47,920
+دستيرل واتر agar is dried hydrophilic jelly-like
+
+115
+00:08:47,920 --> 00:08:52,960
+substance بقولّي الأجار قلنا عبارة عن solidifying
+
+116
+00:08:52,960 --> 00:08:58,320
+agent هو مادة مجففة زي الـ jelly هيدروفليك محبل
+
+117
+00:08:58,320 --> 00:09:02,840
+الماء بيعطي طبعا ال media القوام الصلب عشان ما
+
+118
+00:09:02,840 --> 00:09:07,450
+يكون سائلextracted from various species of red
+
+119
+00:09:07,450 --> 00:09:11,270
+algae بقولك بيستخلص من كائنات مختلفة من التحالب
+
+120
+00:09:11,270 --> 00:09:16,210
+الحمراء it's used as solidifying agent قولنا هو
+
+121
+00:09:16,210 --> 00:09:21,530
+يعتبر solidifying agent عشان تصلب ال media الألجار
+
+122
+00:09:21,530 --> 00:09:27,170
+بيدوب في درجة حرارة 85 وبتصلب عند درجة حرارة 40
+
+123
+00:09:33,040 --> 00:09:38,300
+Gelatin is colorless and odorless substance شو هو
+
+124
+00:09:38,300 --> 00:09:42,140
+الجيلاتين؟ الجيلاتين مادة مايلها رائحة و مايلها
+
+125
+00:09:42,140 --> 00:09:46,080
+لون طيب شو علاقتها بالميديا؟ قولنا زمان كانوا
+
+126
+00:09:46,080 --> 00:09:50,580
+يستخدموه كـ predefine agent لكن حاليا ما بيتم
+
+127
+00:09:50,580 --> 00:09:54,820
+استخدامه تم استبداله بالأجار طيب ليش تم استبداله و
+
+128
+00:09:54,820 --> 00:09:58,860
+ما بيتم استخدامه حاليا؟ لأن في بعض أنواع البكتيريا
+
+129
+00:09:58,860 --> 00:10:03,960
+بتفرز أنزيم الجيلاتينمن اسمه بيكسر الجلدين
+
+130
+00:10:03,960 --> 00:10:08,680
+فبالتالي بيتحول الوسط لثائق that is made from the
+
+131
+00:10:08,680 --> 00:10:13,240
+collagen مصنوع من الكلاجن بيدوب عن درجة حرارة 35
+
+132
+00:10:13,240 --> 00:10:19,520
+ألف في ال device وبيتطلب عن درجات حرارة أقل micro
+
+133
+00:10:19,520 --> 00:10:23,000
+organism are grown and subculture on different
+
+134
+00:10:23,000 --> 00:10:26,440
+type of chemical media بقول إن ال micro organism
+
+135
+00:10:26,440 --> 00:10:30,960
+بنميها و بعملها subculture على أوصات غذائية مختلفة
+
+136
+00:10:31,600 --> 00:10:35,560
+sub culture شو يعني sub culture؟ وشو الفرق بينها
+
+137
+00:10:35,560 --> 00:10:40,200
+وبين ال culture؟ لو أنا أخدت عينة ديوران وزرعتها
+
+138
+00:10:40,200 --> 00:10:44,640
+على وسط بحكي culture لكن لو زرعتها كمان مرة على
+
+139
+00:10:44,640 --> 00:10:49,480
+طبق تاني بحكي sub culture ال media بقولي ممكن تكون
+
+140
+00:10:49,480 --> 00:10:52,420
+chemically defined شو يعني chemically defined؟
+
+141
+00:10:52,420 --> 00:10:57,100
+يعني non concentration مكونات ال media كميات
+
+142
+00:10:57,100 --> 00:11:02,440
+معروفةممكن تكون الـ Media كيميكا دي Undefined
+
+143
+00:11:02,440 --> 00:11:07,480
+النسب تبعتها مش معروفة Unknown proportion a medium
+
+144
+00:11:07,480 --> 00:11:12,700
+is sterilized بس يعني sterilization يعني living
+
+145
+00:11:12,700 --> 00:11:17,820
+organism removed الـ Media قولنا كل أدواتي بيتم
+
+146
+00:11:17,820 --> 00:11:21,240
+تعقيمها والـ Media لازم اني انا اعقمها من الـ
+
+147
+00:11:21,240 --> 00:11:26,280
+Aseptic technique اني انا اعقمهاطرق تعقيم الـ
+
+148
+00:11:26,280 --> 00:11:29,700
+Media طبعا فين دي أكتر من طريقة لتعقيم الـ Media
+
+149
+00:11:29,700 --> 00:11:33,340
+قبل ما نتكلم عن طرق تعقيم الـ Media بنتكلم كيف أنا
+
+150
+00:11:33,340 --> 00:11:39,600
+بدي أحضر Media لو بدي أحضر الـ Nutrient Agar بقرأ
+
+151
+00:11:39,600 --> 00:11:42,780
+طبعا التعليمات الموجودة على الـ Bottle بتيجي
+
+152
+00:11:42,780 --> 00:11:45,920
+Bottle مكتوب عليها Nutrient Agar هاي الـ Bottle
+
+153
+00:11:45,920 --> 00:11:50,900
+Powder بقرأ التعليمات بوظن حسب الكمية اللي أنا بدي
+
+154
+00:11:50,900 --> 00:11:55,920
+إياهابعد ما أوزن بحط الوزنة في فلوسك بضيف ضد ال
+
+155
+00:11:55,920 --> 00:12:00,800
+water حسب الكمية و بحركها بعدين بحطها على ال hot
+
+156
+00:12:00,800 --> 00:12:05,420
+plate في زر لا الحرارة و زر لا السرعة طبعا قبلها
+
+157
+00:12:05,420 --> 00:12:09,780
+بنحط في الفلوسك مغناطيس حتى تدوب ال powder لما
+
+158
+00:12:09,780 --> 00:12:13,940
+تدوب منيح ال media بدخل الفلوسك على ال auto-clamp
+
+159
+00:12:13,940 --> 00:12:18,700
+ال auto-clamp هيعقم ليه ال media و يقتل كل ال
+
+160
+00:12:18,700 --> 00:12:25,150
+microorganism بما فيهملسباري هاك الـ media طلعت
+
+161
+00:12:25,150 --> 00:12:29,050
+معقّمة هاي من الـ Aseptic technique في طرق أخرى
+
+162
+00:12:29,050 --> 00:12:34,370
+منها الـ oven filtration UV exposure إيثالين 5 لكن
+
+163
+00:12:34,370 --> 00:12:38,070
+الـ most common اللي بيتم استخدامها الـ auto
+
+164
+00:12:38,070 --> 00:12:44,430
+-eclipse Other sterilization method طرق أخرى
+
+165
+00:12:44,430 --> 00:12:48,990
+للتعقيم إفلام انتجر عشان التوحشChemicals المواد
+
+166
+00:12:48,990 --> 00:12:52,270
+الكيميائية Ozone نيتروجين ديوكسايد هيدروجين
+
+167
+00:12:52,270 --> 00:12:55,770
+دروكسايد هذا الـ autoplayer used for sterilization
+
+168
+00:12:55,770 --> 00:13:01,110
+بعقم من خلاله الـ media بيتم قتل كل ال micro
+
+169
+00:13:01,110 --> 00:13:06,010
+organism بما فيهم الصغار تحت درجة حرارة عالية وضغط
+
+170
+00:13:06,010 --> 00:13:10,730
+مرتفع هذا هو الـ UV exposure الـ UV بتعمل على قتل
+
+171
+00:13:10,730 --> 00:13:14,990
+ال micro organism بالنسبة لهذه الطريقة هي ال
+
+172
+00:13:14,990 --> 00:13:19,420
+filtrationبكون محضرة ميديا و بحطها بـ cup و بسحب
+
+173
+00:13:19,420 --> 00:13:23,820
+الميديا بالسرنجة و بروح بلزق filter membrane زي
+
+174
+00:13:23,820 --> 00:13:27,940
+اللي بالصورة هي ال filter membrane بالسرنجة و بنزل
+
+175
+00:13:27,940 --> 00:13:32,780
+شويه شويه هيك بتتفلتر الميديا و أكيد بنزلها في الـ
+
+176
+00:13:32,780 --> 00:13:37,920
+sterile cup من الأمثلة على الميديا اللي أنا بستخدم
+
+177
+00:13:37,920 --> 00:13:42,660
+طريقة ال filtration لتحقيمها اليوريا أجار اليوريا
+
+178
+00:13:42,660 --> 00:13:47,780
+أجاربيتم تعقيمها باستخدام ال filtration لأن لو
+
+179
+00:13:47,780 --> 00:13:52,600
+دخلت ال auto-clamp بتتكسر هذه ال media فبستخدم
+
+180
+00:13:52,600 --> 00:13:57,700
+طريقة ال filtration هاي ال ethylene oxide وهذا هو
+
+181
+00:13:57,700 --> 00:14:03,780
+ال offer auto
+
+182
+00:14:03,780 --> 00:14:07,220
+-clamp the principle of sterilization in an auto
+
+183
+00:14:07,220 --> 00:14:12,010
+-clamp is that steam under pressure قولي إنهمبدأ
+
+184
+00:14:12,010 --> 00:14:15,630
+عمل الـ Autoclaval Sterilization steam under
+
+185
+00:14:15,630 --> 00:14:21,990
+pressure بيرفع درجة الحرارة لـ 121 بالتدريج لما
+
+186
+00:14:21,990 --> 00:14:28,710
+تصل لـ 121 بيبدأ يحسب الـ 15 minutes درجة حرارة
+
+187
+00:14:28,710 --> 00:14:33,050
+عالية 121 مع ضغط مرتفع كل ال micro organism بتموت
+
+188
+00:14:33,050 --> 00:14:37,250
+بما فيهم بسبع بعد ما تخلص الـ 15 minutes تنخسر
+
+189
+00:14:37,250 --> 00:14:41,580
+درجة الحرارة بالتدريجهك الميديا صارت استيرايل
+
+190
+00:14:41,580 --> 00:14:47,420
+معظمة بطلع الميديا بستناها تبرد شوية أقدر أني أنا
+
+191
+00:14:47,420 --> 00:14:51,760
+أمسكها بصوبها في petri dish جنب اللهب طبعا لازم
+
+192
+00:14:51,760 --> 00:14:54,420
+جنب اللهب لأنه من الـ Aseptic technique
+
+193
+00:14:54,420 --> 00:14:59,540
+sterilization of equipment and material wire loop
+
+194
+00:14:59,540 --> 00:15:03,660
+اللي هو الانيكلاتينج loop or iniculating needle
+
+195
+00:15:03,660 --> 00:15:09,010
+heat to redness inبزنسين بو عرنر إفلام كيف بدي أنا
+
+196
+00:15:09,010 --> 00:15:12,890
+أعقم الـ wire loop و اللي بحطه على الإفلام لما
+
+197
+00:15:12,890 --> 00:15:17,590
+يتوهج و يصير لونه أحمر هيك بتكون تم تعقيمه لـ
+
+198
+00:15:17,590 --> 00:15:22,890
+glassware الذجاجيات بيتم تعقيمها بالـ oven بالنسبة
+
+199
+00:15:22,890 --> 00:15:28,650
+للـ oven درجة الحرارة بتكون من 160 ل180 درجة
+
+200
+00:15:28,650 --> 00:15:33,310
+سليسيا لمدة ساعتين لـ glassware أنا لما أدخلها على
+
+201
+00:15:33,310 --> 00:15:38,530
+ال ovenبلفها بيه سلفان او ورق زي السلفان ده يسميه
+
+202
+00:15:38,530 --> 00:15:43,090
+grass proof paper او aluminium allowing additional
+
+203
+00:15:43,090 --> 00:15:46,930
+time for items to come to temperature and cool
+
+204
+00:15:46,930 --> 00:15:53,070
+down وقوللي هان انه لما تصل درجة الحرارة حرارة ال
+
+205
+00:15:53,070 --> 00:15:58,770
+oven ل160 او 180 بيبدأ يحسب الساعتين لما اتخلص
+
+206
+00:15:58,770 --> 00:16:03,300
+الساعتين بتنزل درجة الحرارة وبيفتح الجهازبالنسبة
+
+207
+00:16:03,300 --> 00:16:08,840
+لـ Plastic بتريدش بتيجي من الشركة بارد بتكون معقمة
+
+208
+00:16:08,840 --> 00:16:13,580
+استيرايل Culture Media and Solutions Culture Media
+
+209
+00:16:13,580 --> 00:16:17,300
+قلنا بيتم تعقيمها في الـ Autoclave solution زي الـ
+
+210
+00:16:17,300 --> 00:16:20,460
+Normal Saline كذلك بيتم تعقيمه في الـ Autoclave
+
+211
+00:16:20,460 --> 00:16:23,580
+اللي هو الـ Pressure Cooker قلنا زي فنجار تفضغط
+
+212
+00:16:23,580 --> 00:16:27,880
+الـ Glass Breeder اللي هو الألشاب الـMetal Forceps
+
+213
+00:16:27,880 --> 00:16:32,190
+الـ Forceps بيتم استخدامه اللي هو الملقطفي فحص الـ
+
+214
+00:16:32,190 --> 00:16:37,430
+Antibiotics بحطه فيه كحوس بعدين بعرضه على إلهام
+
+215
+00:16:37,430 --> 00:16:45,130
+Flaming in Alcohol Sterile
+
+216
+00:16:45,130 --> 00:16:48,550
+Medium هي Medium لكن بعد ما طلعتها من الـ Auto
+
+217
+00:16:48,550 --> 00:16:53,550
+class تكون الوسط خالي من كل ال micro organism مافي
+
+218
+00:16:53,550 --> 00:16:57,230
+عندي living microorganism is one which is free of
+
+219
+00:16:57,230 --> 00:17:02,060
+all life formsIt is usually sterilized by heating
+
+220
+00:17:02,060 --> 00:17:05,480
+it to a temperature at which all contaminant
+
+221
+00:17:05,480 --> 00:17:09,060
+microorganisms are destroyed كل الميكروعجنزم راح
+
+222
+00:17:09,060 --> 00:17:13,880
+تتذمر في ما فيهم بسبوع Colony is the smallest
+
+223
+00:17:13,880 --> 00:17:18,720
+bacterial unit that can be seen with the naked eye
+
+224
+00:17:18,720 --> 00:17:22,220
+هي أصغر واحدة بكتيرية ممكن أنا أشوفها قولنا ال
+
+225
+00:17:22,220 --> 00:17:25,780
+isolated colony بحصل عليها من الربع الرابع لو أنا
+
+226
+00:17:25,780 --> 00:17:30,780
+زرعت بطريقة الأربع cultureلو إجتني عينة يورينز
+
+227
+00:17:30,780 --> 00:17:34,320
+زرعتها أول مرة بسميها culture يبقى هي جزء من
+
+228
+00:17:34,320 --> 00:17:38,880
+العينة is part of a specimen الـ ground بنميها in
+
+229
+00:17:38,880 --> 00:17:43,220
+cultural media بيتم زراعتها و تنميتها على cultural
+
+230
+00:17:43,220 --> 00:17:47,360
+media طيب شو تعريف الـ cultural media is a medium
+
+231
+00:17:47,360 --> 00:17:52,880
+هي وسط liquid أو ثائل that contain nutrients to
+
+232
+00:17:52,880 --> 00:17:57,120
+grow bacteria in vitro فيها مغذيات لازمة لنمو
+
+233
+00:17:57,120 --> 00:18:01,780
+البكتيرياليس لازم أزرع و أنمي البكتيريا فين دي
+
+234
+00:18:01,780 --> 00:18:05,860
+سببان الصف الأول sometimes we cannot identify with
+
+235
+00:18:05,860 --> 00:18:09,820
+microscopical examination directly لأنه بال direct
+
+236
+00:18:09,820 --> 00:18:13,540
+examination اسمه direct بجيب العينة اللي بدي
+
+237
+00:18:13,540 --> 00:18:17,860
+أبحثها على سبيل المثال عينة urine بcenterها باخد
+
+238
+00:18:17,860 --> 00:18:20,960
+ال sediments بشوف تحت ال microscope هذا اسمه
+
+239
+00:18:20,960 --> 00:18:24,800
+direct examination فقط هشوف عندي بكتيريا أو لأ
+
+240
+00:18:24,800 --> 00:18:29,440
+ممكن أتعرف على شكلهاأو على هل هي gram positive أو
+
+241
+00:18:29,440 --> 00:18:32,780
+gram negative من خلال صباغة ال slide لكن ما بقدر
+
+242
+00:18:32,780 --> 00:18:38,660
+أتعرف بشكل نهائي على البكتيريا لازم أعزلها وأنميها
+
+243
+00:18:38,660 --> 00:18:43,060
+وأجري عليها فحوصات كيميائية يتواصلنا للتعريف
+
+244
+00:18:43,060 --> 00:18:47,960
+النهائي سبب التاني sometimes we do culture for
+
+245
+00:18:47,960 --> 00:18:52,160
+antibiotic sensitivity تستهيب ان بعد ما أعرف أي
+
+246
+00:18:52,160 --> 00:18:57,490
+بكتيريا لازم أعمل فحص المضادات الحيوية عشان أشوفهي
+
+247
+00:18:57,490 --> 00:19:02,730
+البكتيريا لأي مبادح حيوي هتستجيب عشان الدكتور يوصف
+
+248
+00:19:02,730 --> 00:19:06,590
+العلاج للمريض في الفحص بعتمد أنه أنا بدي أجيب
+
+249
+00:19:06,590 --> 00:19:11,730
+البكتيريا اللي نميتها وعرفتها بزراها على طبق بشكل
+
+250
+00:19:11,730 --> 00:19:15,770
+continuous بدي أتأكد أن الطبق كله زرعت عليه
+
+251
+00:19:15,770 --> 00:19:20,870
+بكتيريا بعدها أحط قراس Antibiotic فوق البكتيريا
+
+252
+00:19:20,870 --> 00:19:25,070
+اللي أنا زرعتها بحطها في ال incubator بعد 24 ساعة
+
+253
+00:19:25,070 --> 00:19:30,090
+بشوف النتيجةإذا كانت البكتيريا حساسة لـ Antibiotic
+
+254
+00:19:30,090 --> 00:19:34,370
+بشوف zone of inhibition منطقة فاضية حوالين الـ
+
+255
+00:19:34,370 --> 00:19:37,770
+Antibiotic يعني الـ Antibiotic قتل هذه البكتيريا
+
+256
+00:19:37,770 --> 00:19:44,330
+لو كانت مقاومة للـ Antibiotic هشوف نمول البكتيريا
+
+257
+00:19:44,330 --> 00:19:48,210
+حوالين الـ Antibiotic أكيد الدكتور هيختار الـ
+
+258
+00:19:48,210 --> 00:19:52,870
+Antibiotic اللي كان طبعا أو البكتيريا اللي كانت
+
+259
+00:19:52,870 --> 00:19:57,770
+حساسة لـ Antibioticمش المقاومة لأنه مش هيستجيب له
+
+260
+00:19:57,770 --> 00:20:01,870
+خاطرات الـ Media أكيد الـ Media بتدعم نمو
+
+261
+00:20:01,870 --> 00:20:07,050
+البكتيريا بتحتوي على مواد غذائية الـ PF للميديا
+
+262
+00:20:07,050 --> 00:20:11,550
+بيكون neutral to slightly alkaline درجة الحرارة
+
+263
+00:20:11,550 --> 00:20:17,130
+لازم تكون مناسبة لأن البكتيريا بتنمو عن درجة حرارة
+
+264
+00:20:17,130 --> 00:20:17,450
+37
+
+265
+00:20:20,740 --> 00:20:25,080
+بشوف zone of inhibition حوالين ال antibiotic يعني
+
+266
+00:20:25,080 --> 00:20:29,960
+ال antibiotic قطل high البكتيريا high معناها ان هي
+
+267
+00:20:29,960 --> 00:20:34,840
+حساسة لكن لو كانت مقاومة ل antibiotic معين هشوف
+
+268
+00:20:34,840 --> 00:20:38,340
+نمو البكتيريا حوالين ال antibiotic هان في نمو
+
+269
+00:20:38,340 --> 00:20:41,620
+حوالين ال antibiotic يبقى مقاومة لكن هان zone of
+
+270
+00:20:41,620 --> 00:20:46,720
+inhibition يبقى حساسة فالدكتور رح يختارالـ
+
+271
+00:20:46,720 --> 00:20:52,720
+Antibiotic الحساس مش المقاوم لأنه مش هيستجيب إذن
+
+272
+00:20:52,720 --> 00:20:58,980
+ال
+
+273
+00:20:58,980 --> 00:21:02,200
+media بنصمسها بناء على تلت معايير حسب ال
+
+274
+00:21:02,200 --> 00:21:05,680
+consistency حسب القوام ال nutrition المواد
+
+275
+00:21:05,680 --> 00:21:09,660
+الغذائية ال function الوظيفة بالنسبة للقوام بصمسها
+
+276
+00:21:09,660 --> 00:21:15,930
+لسولد سيمي سولد براس سولد نسبة الأجار بتكونمن 1.5%
+
+277
+00:21:15,930 --> 00:21:20,510
+لـ 2% أجار من الأمثلة عليها لـ Nutrients أجار ال
+
+278
+00:21:20,510 --> 00:21:24,090
+chemical lead نسبة الأجار بتكون 4 من 10% من
+
+279
+00:21:24,090 --> 00:21:28,110
+الأمثلة عليها ال media SIM أو اللي استخدمناها في
+
+280
+00:21:28,110 --> 00:21:33,050
+فحص المجتلد ال broth ما فيها أجار، نسبة الأجار
+
+281
+00:21:33,050 --> 00:21:36,230
+Zero من الأمثلة عليها لـ Nutrients broth أو
+
+282
+00:21:36,230 --> 00:21:39,650
+النيترات البروث أو ال brain hard infusion البروث
+
+283
+00:21:40,640 --> 00:21:43,900
+تاني معيار حسب الـ Nutrition حسب المواد الغذائية
+
+284
+00:21:43,900 --> 00:21:48,780
+بصنفها إما لـ Simple أو Complex الـ Simple بتحتوي
+
+285
+00:21:48,780 --> 00:21:53,300
+على مغذي واحد فقط مصدر واحد للتغذية من الأمثلة
+
+286
+00:21:53,300 --> 00:21:57,040
+عليها الـ Beton Water Broth في هذه الميديا الـ
+
+287
+00:21:57,040 --> 00:22:01,400
+Beton هو مصدر التغذية الوحيد الـ Complex بكون فيه
+
+288
+00:22:01,400 --> 00:22:05,120
+أكثر من مصدر للتغذية من الأمثلة عليها الـ Blood
+
+289
+00:22:05,120 --> 00:22:09,570
+Agaricتالت ميار على حسب الـ Function تقسم إلى
+
+290
+00:22:09,570 --> 00:22:12,870
+General Selective Differential Transport
+
+291
+00:22:12,870 --> 00:22:17,710
+Enrichment General من اسمها General بتنمي معظم
+
+292
+00:22:17,710 --> 00:22:20,710
+أنواع البكتيريا نيوتريان أجار من الأمثلة عليها
+
+293
+00:22:20,710 --> 00:22:25,950
+Selective اختيارية بتنمي أنواع معينة من البكتيريا
+
+294
+00:22:25,950 --> 00:22:30,690
+متشابهة في الحصاء يعني حسب حصية معينة من الأمثلة
+
+295
+00:22:30,690 --> 00:22:35,340
+عليها الـ Manitoulin Source Agile MSAhigh الـ MSA
+
+296
+00:22:35,340 --> 00:22:38,760
+بتنمي فقط البكتيريا اللي بتتحمل درجة الملوحة
+
+297
+00:22:38,760 --> 00:22:41,980
+العالية الـ high concentration of salts يبقى
+
+298
+00:22:41,980 --> 00:22:47,000
+الخاصية هي تحمل درجة الملوحة الـ NACL الموجود في
+
+299
+00:22:47,000 --> 00:22:53,140
+الـ media تركيزه 7.5% أكيد بقى البكتيريا مارح
+
+300
+00:22:53,140 --> 00:22:56,700
+تتحمل هذه الدرجة العالية من الـ salt فبالتالي مارح
+
+301
+00:22:56,700 --> 00:23:01,440
+تلمس الـ differential من اسمها differential هتميز
+
+302
+00:23:01,440 --> 00:23:07,080
+أكتر من نوعمن الـ Bacteria بتعطي كل نوع لون معين
+
+303
+00:23:07,080 --> 00:23:10,060
+من الأمثلة عليها الـ Manifold false eggs الـ
+
+304
+00:23:10,060 --> 00:23:12,940
+Manifold false أجار تعتبر selective and
+
+305
+00:23:12,940 --> 00:23:17,300
+differential ال transport media ال transport media
+
+306
+00:23:17,300 --> 00:23:21,220
+من الأمثلة عليها ال securette securette transport
+
+307
+00:23:21,220 --> 00:23:27,200
+media هو عبارة عن أسواق فيها media هي تستخدم لو ما
+
+308
+00:23:27,200 --> 00:23:31,000
+كان ضمن العين بعيد عن المختبر أكيد بستخدم ال
+
+309
+00:23:31,000 --> 00:23:35,730
+transport mediaتحفظ البكتيريا عشان ما تموت خلال
+
+310
+00:23:35,730 --> 00:23:39,190
+عملية المقل قبل ما أنا أشتغلها لو أنا ماعندي
+
+311
+00:23:39,190 --> 00:23:42,550
+Transport Media ممكن أني أحطها في Normal Saline
+
+312
+00:23:42,550 --> 00:23:46,650
+الـ Normal Saline هيحفظ البكتيريا من إنها اتموت
+
+313
+00:23:46,650 --> 00:23:51,430
+خامس نوع اللي هو الـ Enrich الـ Enrich في أنها
+
+314
+00:23:51,430 --> 00:23:55,950
+تحتاج لبعض الإضافات بعد ما يتم تحضير الـ Media لأن
+
+315
+00:23:55,950 --> 00:24:00,370
+في بعض أنواع البكتيريا تعتبر fastidious مدلة لأ
+
+316
+00:24:00,370 --> 00:24:06,940
+عشان تنمو تحتاج إلىبعض الإضافات من الإضافات ممكن
+
+317
+00:24:06,940 --> 00:24:11,940
+نضيف blood ممكن نضيف egg ممكن نضيف serum بالنسبة
+
+318
+00:24:11,940 --> 00:24:16,200
+لأمثلة على ال enriched ال blood media او ال
+
+319
+00:24:16,200 --> 00:24:20,940
+chocolate media بالنسبة لل blood نضيف البلد لكن
+
+320
+00:24:20,940 --> 00:24:24,260
+البلد بعد ما انا اطلع طبعا هي هواكس بحد ال
+
+321
+00:24:24,260 --> 00:24:28,140
+nutrient agar بعد ما انا اطلعها من ال autoclave
+
+322
+00:24:28,140 --> 00:24:33,700
+بخليها تبرد بضيف البلد بعد ما تبردبنسبة خمسة في
+
+323
+00:24:33,700 --> 00:24:39,900
+المية يعني الخمسة بدها 100 مل الـ nutrient agar لو
+
+324
+00:24:39,900 --> 00:24:44,460
+أنا حضرت ألف ملي من الـ nutrient agar بحط خمسين مل
+
+325
+00:24:44,460 --> 00:24:49,360
+من الـ blood على الألف مل من الـ nutrient agar لكن
+
+326
+00:24:49,360 --> 00:24:54,720
+للشوكولات أنا بضيف الـ blood هو اللي بعد ما أطلع
+
+327
+00:24:54,720 --> 00:24:58,140
+ال media من الـ autoclave وهي سخنة ليش؟ لأن درجة
+
+328
+00:24:58,140 --> 00:25:02,200
+الحرارة العالية بتعمل cumulative لل RPTsبتطلع
+
+329
+00:25:02,200 --> 00:25:08,020
+فاكتور Exovacter في المهمين لنمو بعض أنواع
+
+330
+00:25:08,020 --> 00:25:12,240
+البكتيريا اللي هي الفاصلية البكتيريا ال enrichment
+
+331
+00:25:12,240 --> 00:25:17,060
+ال enrichment هي بتعمل inhibition لنمو ال unwanted
+
+332
+00:25:17,060 --> 00:25:21,520
+بكتيريا بالتالي بتسمح لنمو فقط ال wanted بكتيريا
+
+333
+00:25:21,520 --> 00:25:25,240
+أنها تنمو بكل أرياحية مثل الأمثلة عليها Selenite
+
+334
+00:25:25,240 --> 00:25:29,670
+of Fibrose بتنميالـ Salmonella الألكالاين بيبتون
+
+335
+00:25:29,670 --> 00:25:36,950
+واتر اللي بتنمي الـ Vibrio cholera هاي
+
+336
+00:25:36,950 --> 00:25:41,870
+هي ال transport media هي كبتيجي بكات بتكون معقمة
+
+337
+00:25:41,870 --> 00:25:46,570
+استيرال لو أنا بدي أخد مسحة باخد المسحة بالسواب
+
+338
+00:25:46,570 --> 00:25:51,210
+هذا بعد ما أخد المسحة بحطه في التيوب هذا اللي
+
+339
+00:25:51,210 --> 00:25:52,870
+موجود في الميديا
+
+340
+00:25:57,380 --> 00:26:01,020
+Forms of Culture Media أشكال الـ Culture Media أول
+
+341
+00:26:01,020 --> 00:26:05,580
+شكل البروث الـ Q الميديا ممكن تكون بروث طبعا
+
+342
+00:26:05,580 --> 00:26:10,280
+البروث بحطها بـ Q RQ's containing a liquid media
+
+343
+00:26:10,280 --> 00:26:14,700
+عنابيب تحتوي على liquid media على ميديا سائلة a
+
+344
+00:26:14,700 --> 00:26:19,580
+typical nutrient containing بروث ميديا تشقق سيبروث
+
+345
+00:26:19,580 --> 00:26:25,370
+اتنوثيانت بروث من الأمثلة على البروث الـ Qطيب بعد
+
+346
+00:26:25,370 --> 00:26:28,870
+ما أزرع البكتيريا في بروست يو كيف أعرف نمط
+
+347
+00:26:28,870 --> 00:26:33,330
+البكتيريا أو لأ و اللي بتلاقي انه موبى تلت أشكال
+
+348
+00:26:33,330 --> 00:26:37,930
+ممكن تلاحظي أزوان ممكن تلاقيه كشكل او a
+
+349
+00:26:37,930 --> 00:26:41,690
+combination of three forms أو تلاقيهم مستمعين
+
+350
+00:26:41,690 --> 00:26:48,310
+التلت أشكال نتعرف على التلت أشكال أول واحد البريكل
+
+351
+00:26:48,310 --> 00:26:52,170
+a mass of organism is floating on top of the
+
+352
+00:26:52,170 --> 00:26:57,500
+prostateبقوللي بتكون البكتيريا بتنمو على السطح
+
+353
+00:26:57,500 --> 00:27:02,620
+بتطفو على السطح turbidity the organism appear as a
+
+354
+00:27:02,620 --> 00:27:06,100
+general cloudiness throughout the breath بتكون
+
+355
+00:27:06,100 --> 00:27:11,360
+امعكزة لتيوب sediment a mass of organism كتلة من
+
+356
+00:27:11,360 --> 00:27:15,480
+الخلايا بتكون at the bottom of the tube بتكون
+
+357
+00:27:15,480 --> 00:27:20,870
+متركزة في القاع طبعا الاختلافحسب نوع البكتيريا
+
+358
+00:27:20,870 --> 00:27:25,330
+واختلاف احتياجاتها للأكسجين فمثلًا الـ pellicle
+
+359
+00:27:25,330 --> 00:27:29,950
+بتكون aerobic لكن ال sediment بتكون anaerobic
+
+360
+00:27:29,950 --> 00:27:35,730
+طريقة الزراعة باخذ ال-iniculum أو البكتيريا وبعمل
+
+361
+00:27:35,730 --> 00:27:37,350
+mix لبراس EQ
+
+362
+00:27:42,170 --> 00:27:46,390
+بالنسبة لأشكال انمو في البروس a tube لو كانت
+
+363
+00:27:46,390 --> 00:27:50,510
+مجموعة الخلايا مترسبة في القاع يبقى بتكون sediment
+
+364
+00:27:50,510 --> 00:27:54,270
+لو كانت طافية على السطح bellicle بنلاحظ بنفس ال
+
+365
+00:27:54,270 --> 00:27:58,650
+tube في عندي كمان مجموعة من الأورجانيزم مترسب في
+
+366
+00:27:58,650 --> 00:28:01,650
+القاع يبقى sediment في هاي ال tube في عندي
+
+367
+00:28:01,650 --> 00:28:05,010
+bellicle and sediment في ال slide القبل قلنا ممكن
+
+368
+00:28:05,010 --> 00:28:09,910
+ألاقي انمو بشكل أو بأكتر من شكل أغر شكل التربي دي
+
+369
+00:28:09,910 --> 00:28:14,230
+تيب كل tube امعكرخلصنا البروتاتيوب أو الشكل من
+
+370
+00:28:14,230 --> 00:28:18,110
+أشكال الـ culture ميدياني جلدان شكل الـ slant tube
+
+371
+00:28:18,110 --> 00:28:22,310
+are tubes containing a nutrient medium بلصق
+
+372
+00:28:22,310 --> 00:28:27,470
+صليدفاين أجار أنابيب تحتوي على الـ nutrient media
+
+373
+00:28:27,470 --> 00:28:33,130
+على وصف غذائي مع صليدفاين أجار في البروتاتيوب كان
+
+374
+00:28:33,130 --> 00:28:37,070
+في عندي وصف غذائي لكن ماكن في عندي صليدفاين أجار
+
+375
+00:28:37,070 --> 00:28:40,750
+ماكن في عندي أجار كانت في بروت ثائل هذه الفرق
+
+376
+00:28:40,750 --> 00:28:47,390
+بينها وبينبالنسبة للإسلانتي تيوب كيف احصل على شكل
+
+377
+00:28:47,390 --> 00:28:55,190
+الإسلان؟ شكل الإسلان بيكون هاك لو رسمناه هي التيوب
+
+378
+00:28:55,190 --> 00:29:02,690
+بيكون مائل كيف احصل على شكل الإسلان؟ بتركها تتصلب
+
+379
+00:29:02,690 --> 00:29:05,630
+في زاوية يعني بعد ما طلعها من الـ autoclave بحطها
+
+380
+00:29:05,630 --> 00:29:09,550
+في زاوية عشان تتصلب في زاوية كيف بصراع الإسلانتي
+
+381
+00:29:09,550 --> 00:29:14,870
+تيوب؟بدخل بعمل طعن للمنطقة اللي تحت بدخل بالـ
+
+382
+00:29:14,870 --> 00:29:18,750
+Iniculating Needle بعمل طعن للمنطقة اللي تحت بعدين
+
+383
+00:29:18,750 --> 00:29:22,630
+بطلع بعمل ريكزاج للمنطقة المائية هذا بيكون السطح
+
+384
+00:29:22,630 --> 00:29:27,550
+بعمل ريكزاج عشان أزرع على هذا السطح البكتيريا طب
+
+385
+00:29:27,550 --> 00:29:32,030
+ليش بزرع البكتيريا بطريقة الـ Flat Etude؟ in order
+
+386
+00:29:32,030 --> 00:29:36,510
+to get a flat iniculating surface it used for
+
+387
+00:29:36,510 --> 00:29:40,840
+transport and storageهذه الحالة بيكون عندى مساحة
+
+388
+00:29:40,840 --> 00:29:44,900
+سطح أكبر يبقى بحصل على كمية أكبر من البكتيريا
+
+389
+00:29:44,900 --> 00:29:49,660
+بيكون مناسب في حالة ال transport في حالة النقل أو
+
+390
+00:29:49,660 --> 00:29:52,860
+لل storage التخزين لو بدي أخزن البكتيريا ال
+
+391
+00:29:52,860 --> 00:29:57,340
+slanted tube مناسبة لأنها بتوفر مساحة سطح أكبر
+
+392
+00:29:57,340 --> 00:30:01,760
+تالت شكل من أشكال ال cultural media للـ tab Q4DP
+
+393
+00:30:01,760 --> 00:30:05,840
+نفس ال slanted tube أنابيب تحتوي على وسط غذائي
+
+394
+00:30:05,840 --> 00:30:10,520
+معهد للـ Designing أجارلكن الفرق الإختلاف عن الـ
+
+395
+00:30:10,520 --> 00:30:14,160
+slanted tube إنها مش بزاوية بتكون عادي بطلّحها من
+
+396
+00:30:14,160 --> 00:30:18,180
+الـ autoclave بحطها في الرّاك تتصلب، بتركها تتصلب
+
+397
+00:30:18,180 --> 00:30:23,200
+لأن طريقة الزراعة انيكولاتية بيستعمل تطعن
+
+398
+00:30:23,200 --> 00:30:27,580
+الانيكولام إلى أجار الانيكولام هي المسحة اللي أنا
+
+399
+00:30:27,580 --> 00:30:31,560
+أخدتها أو البكتيريا بطعنها في الأجار بفحص الـ
+
+400
+00:30:31,560 --> 00:30:37,290
+motility أجار إبلات خلّصنا من ال tubeالـ tube إما
+
+401
+00:30:37,290 --> 00:30:41,150
+يكون بروس ممكن يكون slanted tube ممكن يكون stab
+
+402
+00:30:41,150 --> 00:30:45,250
+tube لكن البلاد فقط أجار لأنه مابقدر أحط بروس في
+
+403
+00:30:45,250 --> 00:30:50,350
+البلاد are sterile petri-blets قولنا إنه الـ petri
+
+404
+00:30:50,350 --> 00:30:54,750
+-blets بيجيب استيرايل معقّم في bags that are
+
+405
+00:30:54,750 --> 00:30:59,070
+aseptically filled with a melted sterile agar
+
+406
+00:30:59,070 --> 00:31:03,990
+media and allowed to solidify أنا بصب الميديا في
+
+407
+00:31:03,990 --> 00:31:09,930
+الأطباقبشكل طبعا بشكل اني انا امنع يكون تماناش
+
+408
+00:31:09,930 --> 00:31:13,950
+Aseptically بعمل كل الإجراءات اللازمة لمنع يكون
+
+409
+00:31:13,950 --> 00:31:17,830
+تماناش بصب ال media في الأطباق هاي ال media بتكون
+
+410
+00:31:17,830 --> 00:31:23,070
+استيرال معقمة وبعدين بتركها تتصلق بلايز are much
+
+411
+00:31:23,070 --> 00:31:27,610
+less confining than slant and stack بقولي البلايز
+
+412
+00:31:27,610 --> 00:31:31,210
+بتكون أقل قيود من ال slant و ال stack عشان هي أنا
+
+413
+00:31:31,210 --> 00:31:35,670
+بستخدمها in the culture inالزراعة عشان أحصل على
+
+414
+00:31:35,670 --> 00:31:39,750
+isolated colony و أقدر أعمل pure culture و أتعرف
+
+415
+00:31:39,750 --> 00:31:46,650
+على البكتيريا سيبراتينج قلنا أنه أنا من خلال طريقة
+
+416
+00:31:46,650 --> 00:31:50,450
+الأرباع بقدر أفصل المستعمرات على البعض و أحصل على
+
+417
+00:31:50,450 --> 00:31:55,090
+isolated colony and counting of microorganisms لو
+
+418
+00:31:55,090 --> 00:31:59,730
+زرعت على إسلام مش هقدر أني أعد ال micro organism
+
+419
+00:31:59,730 --> 00:32:04,550
+لكن بالطبقيا لمساحة وارتيا بقدر أعدفي كمان شغلة
+
+420
+00:32:04,550 --> 00:32:09,650
+تالتة بقدر من خلالها أو بتفيدني فيه البلات اللي هي
+
+421
+00:32:09,650 --> 00:32:14,190
+فحص المضادات الحيوية طبعا الفحص المضادات الحيوية
+
+422
+00:32:14,190 --> 00:32:18,350
+الطبق أفضل ما بقدر أعمل هذا الفحص let you pick up
+
+423
+00:32:18,350 --> 00:32:22,530
+البلات is less confining than slants and steps
+
+424
+00:32:22,530 --> 00:32:26,170
+تستخدم في ال capturing, separating and counting
+
+425
+00:32:26,170 --> 00:32:31,060
+وفي كمان في فحص المضادات الحيويةطرق زراعة لبلاد
+
+426
+00:32:31,060 --> 00:32:40,760
+هيتعرف عليها في آخر المعمل هان
+
+427
+00:32:40,760 --> 00:32:44,120
+أشكال الـ Cultural Media قولنا لتيوب في عندي تلات
+
+428
+00:32:44,120 --> 00:32:47,800
+أشكال ممكن تكون البروست ميديا لكن ما بتحتوي على
+
+429
+00:32:47,800 --> 00:32:53,220
+أجار ممكن تكون أجار Deep اللي هي فيها أصل الـ
+
+430
+00:32:53,220 --> 00:32:57,500
+Dividing Agent أجارلكن بتكون بشكل عادي بتركها على
+
+431
+00:32:57,500 --> 00:33:02,100
+ذاك تتطلب فتاخد الشكل العادي بالنسبة للأجار slant
+
+432
+00:33:02,100 --> 00:33:05,220
+بتفرق عن الأجار دي فيها طبعا في ال device agent
+
+433
+00:33:05,220 --> 00:33:10,900
+لكن بتكون بزاوية بتكون مائلة بالنسبة لبلاد أكيد
+
+434
+00:33:10,900 --> 00:33:17,140
+هتكون أجار إبلات هنأشكال
+
+435
+00:33:17,140 --> 00:33:20,340
+ال culture media اللي فيها أجار اللي هي لبلاد
+
+436
+00:33:20,340 --> 00:33:27,070
+الأجار دي والأجار slantفيها مقارنة لمساحة السطح
+
+437
+00:33:27,070 --> 00:33:30,410
+أكبر مساحة السطح أكيد هتكون الأجاري بلايك بعدين
+
+438
+00:33:30,410 --> 00:33:34,190
+الأجاري slant لكن الأجاري tube مافي عندي أي مساحة
+
+439
+00:33:34,190 --> 00:33:40,970
+سطح كيف بدي أحصل على ال slant tube ممكن أني أنا
+
+440
+00:33:40,970 --> 00:33:44,470
+أحط ال tube بعد ما أطلعها من ال autoclave على أي
+
+441
+00:33:44,470 --> 00:33:49,610
+سطح بشكل مائل فبعد فترة هتتطلب وتعطيني ال slant
+
+442
+00:33:49,610 --> 00:33:53,610
+tubeممكن فيه ان دي جهاز بينحط عليه إجراءات بشكل
+
+443
+00:33:53,610 --> 00:33:57,430
+مائل فبالتالي بيتكون لـ slant tube زي ما احنا
+
+444
+00:33:57,430 --> 00:34:01,710
+ملاحظين في الصورة
+
+445
+00:34:01,710 --> 00:34:05,550
+Aseptic technique يعني كل الإجراءات اللتي يتم
+
+446
+00:34:05,550 --> 00:34:09,070
+اتخاذها لمنع الـ contamination the most commonly
+
+447
+00:34:09,070 --> 00:34:12,590
+used device for moving bacteria is the inculating
+
+448
+00:34:12,590 --> 00:34:16,830
+loop بقول لي إن الـinculating loop هي أدات استخدام
+
+449
+00:34:16,830 --> 00:34:21,210
+لنقل بكتيريا من وسط لآخر وزراعتهاالـ Nucleating
+
+450
+00:34:21,210 --> 00:34:24,610
+loop ممكن أنها تكون من النيكروم النيكروم and alloy
+
+451
+00:34:24,610 --> 00:34:28,730
+هي سبيكة of nickel and chromium أو ممكن تكون من
+
+452
+00:34:28,730 --> 00:34:33,170
+البلاتينيوم wire بالنسبة للـ Nucleating loop
+
+453
+00:34:33,170 --> 00:34:37,230
+بنلاحظ أن في عندي أنا حلقة loop at one end في
+
+454
+00:34:37,230 --> 00:34:40,610
+نهايتها في حلقها الحلقة هي اللي باخد البكتيريا
+
+455
+00:34:40,610 --> 00:34:44,890
+فيها والجزء الثاني اللي هو أنا بمسك وهذا بالنسبة
+
+456
+00:34:44,890 --> 00:34:48,790
+للـ Nucleating loopفي أداء تشبهها اسمها الـ
+
+457
+00:34:48,790 --> 00:34:52,050
+Inculating Needle اللي احنا استخدمناها في الـ
+
+458
+00:34:52,050 --> 00:34:55,850
+Motility هي نفس الشيء لكن مش موجود في Handy Loop
+
+459
+00:34:55,850 --> 00:35:04,110
+مافي Handy حلقة هي Stressed Wire كيف
+
+460
+00:35:04,110 --> 00:35:07,350
+انا بدي اعقم الـ Inculating Loop أو الـ Needle؟
+
+461
+00:35:07,350 --> 00:35:10,750
+استيريليز بواب إنسترمنت بحافظ على جزء الـ Wire في
+
+462
+00:35:10,750 --> 00:35:15,110
+الأسلام حتى يشعروا بالإنسناراش بعقم الأدتان
+
+463
+00:35:15,110 --> 00:35:20,820
+بتعريضهم لللهبلما تتوهج glow يصير لونه أحمر هيك
+
+464
+00:35:20,820 --> 00:35:24,680
+بيكون تم إبتعاقيم the instrument should be allowed
+
+465
+00:35:24,680 --> 00:35:29,060
+to cool قولي بعد ما تم عملية التعقيم بستنى غلق
+
+466
+00:35:29,060 --> 00:35:33,240
+اللوب أو ال needle إنها تبرد لمدة عشر لعشرين
+
+467
+00:35:33,240 --> 00:35:37,660
+second قبل ما أنا أستخدمها why to avoid killing
+
+468
+00:35:37,660 --> 00:35:41,920
+the iniculum عشان ما أقتل ال iniculum ال iniculum
+
+469
+00:35:41,920 --> 00:35:45,290
+المسح اللي أنا أخدتهاin this way all contaminants
+
+470
+00:35:45,290 --> 00:35:49,250
+on the wire are incinerated في هذه الطريقة كل ال
+
+471
+00:35:49,250 --> 00:35:52,990
+contaminants اللي على ال wire أو على ال loop
+
+472
+00:35:52,990 --> 00:35:57,470
+بيصلوا incinerated فيها ملاحظات don't blow on the
+
+473
+00:35:57,470 --> 00:36:00,950
+instrument to cool them ممنوع أني أنفخ على ال loop
+
+474
+00:36:00,950 --> 00:36:05,350
+أو ال needle عشان أخليه يبرد ممنوع don't touch the
+
+475
+00:36:05,350 --> 00:36:09,090
+instrument to a car to cool them ممنوع أعمل touch
+
+476
+00:36:09,090 --> 00:36:12,850
+بالأجار عشان أخليه يبردDon't lay the loop down
+
+477
+00:36:12,850 --> 00:36:17,170
+once it is sterilized ممنوع أخلي اللوب أو الـ
+
+478
+00:36:17,170 --> 00:36:20,470
+needle بعد ما أعقمه أحطه على البرج لأنه بيصير
+
+479
+00:36:20,470 --> 00:36:27,010
+contaminant procedure
+
+480
+00:36:27,010 --> 00:36:31,050
+for aseptically transfer أول شي بدي أعقم اللوب
+
+481
+00:36:31,420 --> 00:36:34,220
+واللي تاني إشي بدون ما تحط اللوب على الـ bench
+
+482
+00:36:34,220 --> 00:36:38,160
+بتنسكي الـ culture tube بتعقم الفوهة بتمريرها على
+
+483
+00:36:38,160 --> 00:36:42,260
+اللهد بسرعة ممنوع أحط الغطاية على الـ bench بضلي
+
+484
+00:36:42,260 --> 00:36:46,500
+مسكاها بإيدي الغطاية بنسكها بنفس الإيدي اللي ماسكا
+
+485
+00:36:46,500 --> 00:36:52,460
+فيها اللوب تالت إشي بدخل اللوب في الـ tube و بذرع
+
+486
+00:36:52,460 --> 00:36:56,700
+رابع إشي بعقم بعد ما أخلص الشغل بعقم الفوهة و
+
+487
+00:36:56,700 --> 00:37:01,620
+بفكرهاخامس شاق بكرر نفس الخطوات في حال أنا ذراعت
+
+488
+00:37:01,620 --> 00:37:08,780
+أكتر من الـ tube لما
+
+489
+00:37:08,780 --> 00:37:13,340
+أخلص الشغل بعقم اللوب و بحطه على ال bench لو أنا
+
+490
+00:37:13,340 --> 00:37:19,720
+فكت أني أنا ما عقمت، بعقم هان
+
+491
+00:37:19,720 --> 00:37:24,400
+الخطوات في صور بعقم اللوب بتعريضه لأ اللهب لما
+
+492
+00:37:24,400 --> 00:37:28,850
+يصير لونه أحمر يتوهجك وعرف تم تعقيمهفخلّيه يبرد
+
+493
+00:37:28,850 --> 00:37:34,870
+قبل ما أستخدمه من 10 ل 20 second عشان أمنع ال
+
+494
+00:37:34,870 --> 00:37:40,830
+killing للانيكولام بمسك
+
+495
+00:37:40,830 --> 00:37:45,630
+ال capture cube برفع الغطاية بعقم الفوهة ممنوع أحط
+
+496
+00:37:45,630 --> 00:37:50,410
+الغطاية على ال bench بزرع بعد ما أخلص الشغل بعقم
+
+497
+00:37:50,410 --> 00:37:52,830
+الفوهة و برجع الغطاية
+
+498
+00:37:58,120 --> 00:38:02,160
+الزائد اللي هبط أول جزء بيكون dark zone of
+
+499
+00:38:02,160 --> 00:38:06,460
+unburned gas هذه المنطقة ما بيصير فيها احتراف
+
+500
+00:38:06,460 --> 00:38:10,060
+بعدين عندي ال orange zone of incomplete combustion
+
+501
+00:38:10,060 --> 00:38:13,600
+بعدين بتيجي منطقة ال blue zone of partial
+
+502
+00:38:13,600 --> 00:38:17,400
+combustion أخر شئ ال colorless zone of complete
+
+503
+00:38:17,400 --> 00:38:21,860
+combustion كل ما طلعنا لفوق بيزيد ال oxygen بيصير
+
+504
+00:38:21,860 --> 00:38:26,400
+sufficient combustion احتراف كامل للغازفبالتالي
+
+505
+00:38:26,400 --> 00:38:30,260
+بتكون شديدة الحرارة hottest يبقى اللي بدنا نعرفه
+
+506
+00:38:30,260 --> 00:38:34,820
+أن التعقيد بيحصل عند المنطقة الزرقه أو المنطقة ال
+
+507
+00:38:34,820 --> 00:38:40,520
+colorless بتميها non-luminous flame بتكون hottest
+
+508
+00:38:40,520 --> 00:38:45,400
+بتكون شديدة الحرارة لكن لل luminous flame بتكون هي
+
+509
+00:38:45,400 --> 00:38:50,660
+المنطقة ال orange not very hotماذا تستخدمها في
+
+510
+00:38:50,660 --> 00:38:55,180
+تعقيم اللوب بستخدم فيما تعقيم اللوب تعقيم عند
+
+511
+00:38:55,180 --> 00:39:00,100
+المنطقة ال blue، الزرق أو ال colorless لأنه بتكون
+
+512
+00:39:00,100 --> 00:39:04,920
+hottest five
+
+513
+00:39:04,920 --> 00:39:10,100
+basic techniques of culturing إنكولات فتحت لتيوب
+
+514
+00:39:10,100 --> 00:39:14,000
+أو لبلات و أخدت إنكولام و زرعت بعد ما زرعت دخلت
+
+515
+00:39:14,000 --> 00:39:17,040
+الطبق على ال incubator و عملت incubation يبقى
+
+516
+00:39:17,040 --> 00:39:21,620
+المرحلة التانية incubationمدت 24 ساعة و أحيانا 48
+
+517
+00:39:21,620 --> 00:39:25,640
+حسب البكتيريا بعد الـ incubation هلاقي نمو
+
+518
+00:39:25,640 --> 00:39:29,660
+للبكتيريا ممكن ينمو أكتر من نوع من البكتيريا باخد
+
+519
+00:39:29,660 --> 00:39:32,920
+كل نوع و بزرعه على طبق هاي المرحلة بسميها
+
+520
+00:39:32,920 --> 00:39:37,360
+isolation يبقى المرحلة التانية ال isolation بعدها
+
+521
+00:39:37,360 --> 00:39:41,480
+هعمل ححوقات تعريفية في مرحلة ال examination عشان
+
+522
+00:39:41,480 --> 00:39:45,240
+أعمل identification للبكتيريا اللي هي المرحلة
+
+523
+00:39:45,240 --> 00:39:45,940
+الأخيرة
+
+524
+00:39:49,790 --> 00:39:53,370
+Pure culture concept to identify bacteria in a
+
+525
+00:39:53,370 --> 00:39:56,670
+clinical sample cannot be done unless isolated
+
+526
+00:39:56,670 --> 00:40:00,290
+colony are used عشان أتعرف على البكتيريا لازم يكون
+
+527
+00:40:00,290 --> 00:40:04,490
+عندي isolated colony ال isolated colony أصلها خلية
+
+528
+00:40:04,490 --> 00:40:09,270
+بكتيرية واحدة كيف بدي أحصل على ال isolated colony؟
+
+529
+00:40:09,270 --> 00:40:13,190
+to obtain well isolated colony it is essential to
+
+530
+00:40:13,190 --> 00:40:17,270
+disperse the inoculum or sample on the surface of
+
+531
+00:40:17,270 --> 00:40:21,570
+an enriched agar plateعشان أحصل على إيزولات
+
+532
+00:40:21,570 --> 00:40:26,110
+الكلوني مهم جدا أوزع العينة على سطح البلاد أجابي
+
+533
+00:40:26,110 --> 00:40:29,830
+So the individual bacteria are well separated from
+
+534
+00:40:29,830 --> 00:40:34,230
+each other هنقدر نفصل البكتيريا عن بعض بطريقة
+
+535
+00:40:34,230 --> 00:40:39,810
+الأربع، هنخطف فبالتالي هنفصل المستعمرات عن بعض، هك
+
+536
+00:40:39,810 --> 00:40:44,790
+بضمن أن الإيزولات الكلوني أصلها خلية بكتيرية واحدة
+
+537
+00:40:45,190 --> 00:40:48,610
+لكن لو أنا أخدت من الرُبع الأول الرُبع الأول بيكون
+
+538
+00:40:48,610 --> 00:40:52,850
+فك المستعمرات فوق بعض ممكن تكون بكتيريا X فوق
+
+539
+00:40:52,850 --> 00:40:56,790
+بكتيريا Y يعني بيكون عندي mixed population روحت
+
+540
+00:40:56,790 --> 00:41:00,910
+أنا أشتغلت حوصات هيكون كل نتائج الحوصات غلط لأنه
+
+541
+00:41:00,910 --> 00:41:04,630
+أشتغلت على بكتيريا X و بكتيريا Y لازم فقط أنا
+
+542
+00:41:04,630 --> 00:41:08,650
+أشتغل على بكتيريا واحدة في ال isolated colony
+
+543
+00:41:08,650 --> 00:41:14,740
+بتكونالمتعمرات بعيدة عن بعض فضمن أن أصلها بكتيريا
+
+544
+00:41:14,740 --> 00:41:19,100
+واحدة
+
+545
+00:41:19,100 --> 00:41:25,660
+Contaminants
+
+546
+00:41:25,660 --> 00:41:29,480
+other microorganisms present in the sample كائنات
+
+547
+00:41:29,480 --> 00:41:33,500
+أخرى موجودة في العين أو في المزرعة فلسبيل المثال
+
+548
+00:41:33,500 --> 00:41:37,990
+إذا طينت يورين وزرعتها لكن ما عقمت اللوبهينمو
+
+549
+00:41:37,990 --> 00:41:41,830
+micro organism على الوساط غير الموجود في عينة الـ
+
+550
+00:41:41,830 --> 00:41:46,390
+urine نتيجة عدم تعقيمي للـ loop isolated colonies
+
+551
+00:41:46,390 --> 00:41:49,390
+a population of millions of cells that are
+
+552
+00:41:49,390 --> 00:41:53,970
+identical and are descended from a single founder
+
+553
+00:41:53,970 --> 00:41:57,690
+cell ال isolated كولوني لو أنا زرعت في طريقة
+
+554
+00:41:57,690 --> 00:42:01,130
+الأربعة هتكون موجودة في القبع الأخير هي ملايين
+
+555
+00:42:01,130 --> 00:42:05,970
+الخلايا المتشابهة اللي أصلها خلية بكتيرية واحدةلو
+
+556
+00:42:05,970 --> 00:42:09,150
+أخدتها وروح اتزرعتها على طبق بسميه طبق pure
+
+557
+00:42:09,150 --> 00:42:13,490
+culture هيك بيصير كل الطبق من بكتيريا واحدة بلاحظ
+
+558
+00:42:13,490 --> 00:42:18,690
+ان كل الكولوني نفس الموارفولوجي Stock culture،
+
+559
+00:42:18,690 --> 00:42:21,570
+stock يعني مخزون، a culture that already contains
+
+560
+00:42:21,570 --> 00:42:26,570
+cells it is used as source of cell from which to
+
+561
+00:42:26,570 --> 00:42:31,450
+manipulate new cultureتحتوي على خلايا تستخدم كمصدر
+
+562
+00:42:31,450 --> 00:42:35,310
+للخلايا البكتيرية عشان تزرع مرة تانية الأطباق اللي
+
+563
+00:42:35,310 --> 00:42:38,990
+بتزرع منها و بتاخدوا بكتيريا تعتبر stock culture
+
+564
+00:42:38,990 --> 00:42:43,930
+عشان تعملوا a new culture ال culture media ممكن
+
+565
+00:42:43,930 --> 00:42:48,750
+تكون rich غنية بمواد غذائية بتنمي كل الأنواع or
+
+566
+00:42:48,750 --> 00:42:52,590
+selective بتنمي نوع معين ال growth فيها مباديات
+
+567
+00:42:52,590 --> 00:42:57,100
+لنمو البكتيريا or inhibitor فيها مصبطاتممكن تكون
+
+568
+00:42:57,100 --> 00:43:01,580
+Liquid او Solid الـ Temperature لازم تكون مناسبة
+
+569
+00:43:01,580 --> 00:43:06,000
+لنمو البكتيريا Source of Energy مفضلة الطاقة زي
+
+570
+00:43:06,000 --> 00:43:10,280
+الجليكوز، اللاكتوز، المانيتال Source of Carbon او
+
+571
+00:43:10,280 --> 00:43:14,240
+نيتروجين أكيد لازم يكون في عندي Carbon Source او
+
+572
+00:43:14,240 --> 00:43:18,060
+Nitrogen Source عشان تنمو البكتيريا وتتكثر من
+
+573
+00:43:18,060 --> 00:43:22,700
+أمثلة على Carbon Source الكربوهيدرات الـNitrogen
+
+574
+00:43:22,700 --> 00:43:26,810
+Source الأمينو أساسيAseptic techniques include
+
+575
+00:43:26,810 --> 00:43:30,090
+sterilization of media لازم اني انا اعقم ال media
+
+576
+00:43:30,090 --> 00:43:34,870
+sterilization of equipment لازم انا اعقم الأدوات
+
+577
+00:43:34,870 --> 00:43:40,050
+proper handling اني انا اشتغل مثلا جنب اللهد ممنوع
+
+578
+00:43:40,050 --> 00:43:43,350
+اني انا انفخ عشان يبرد اللوب اتعقم ال bench قبل
+
+579
+00:43:43,350 --> 00:43:47,410
+الشغل وبعد هذا كلها تعتبر aseptic technique عشان
+
+580
+00:43:47,410 --> 00:43:48,710
+امنع ال contamination
+
+581
+00:43:55,320 --> 00:43:59,720
+الأدوات اللي هستخدمها في الشغل Maniculating Loops
+
+582
+00:43:59,720 --> 00:44:04,580
+لها بنسن للتعقيم stop culture قلنا ان stop culture
+
+583
+00:44:04,580 --> 00:44:08,320
+هي مخزون تحتوي على خلايا عشان انا اعمل a new
+
+584
+00:44:08,320 --> 00:44:12,540
+culture اكيد استرال اجار ابلات اللي انا بدي ازرع
+
+585
+00:44:12,540 --> 00:44:12,840
+عليه
+
+586
+00:44:20,110 --> 00:44:24,510
+كيف طريقة الشغل عشان أحصل على isolated colony أول
+
+587
+00:44:24,510 --> 00:44:29,770
+خطوة بعقّم اللوب باتسنع يبرد باخد انيكلوم من الـ
+
+588
+00:44:29,770 --> 00:44:35,050
+Soap culture وأكيد بعقّم الفوا بفرض الانيكلوم على
+
+589
+00:44:35,050 --> 00:44:38,990
+الرُبع الأول بحاول يكون continuous تقريب عن بعض
+
+590
+00:44:38,990 --> 00:44:42,370
+تاني خطوة بتوقع أنا في الرُبع الأول مش هيكون
+
+591
+00:44:42,370 --> 00:44:46,270
+isolated colony بحاول اني انا ما اضغط على الأجار
+
+592
+00:44:46,270 --> 00:44:50,850
+وانا بصرا أو استخدمت plastic loop بتخلص منهثالث
+
+593
+00:44:50,850 --> 00:44:54,910
+خطوة بقولي تقلبي الطبق 90 درجة و تزرعي الربع
+
+594
+00:44:54,910 --> 00:44:59,070
+التاني تاخدي من الربع الأول و تعملي أخطوط ما ترجعي
+
+595
+00:44:59,070 --> 00:45:07,430
+تاخدي من ال stock لأن أنا بدي أحفظ بكرر
+
+596
+00:45:07,430 --> 00:45:12,450
+نفس الخطوات في الربع التالت و في الربع الرابع
+
+597
+00:45:12,450 --> 00:45:16,570
+بحاول أني أتخلص من ال loop بين ربع و ربع لو أنا
+
+598
+00:45:16,570 --> 00:45:20,820
+استخدمت plastic loop اذا نيكروم loop بعقمالنيكروم
+
+599
+00:45:20,820 --> 00:45:24,900
+لوب آخر إشي بقولي أنا بحط الأطباق في ال incubator
+
+600
+00:45:24,900 --> 00:45:29,240
+upside-down why؟ to prevent condensation حتى أمنع
+
+601
+00:45:29,240 --> 00:45:34,200
+التكاثر فيها الملاحظة أنه أنا لما أفتكر tube بحاول
+
+602
+00:45:34,200 --> 00:45:37,580
+أني أنا ما أمسكها من ال LED لأن ممكن أنها تكون
+
+603
+00:45:37,580 --> 00:45:45,500
+مفتوحة وتنكسر منها هذه
+
+604
+00:45:45,500 --> 00:45:50,590
+الخطوات في سؤال أول إشي بعقم ال loopباخد Eniculum
+
+605
+00:45:50,590 --> 00:45:54,250
+وبذرع الربع الأول بشكل continuous بكونه اقرب عن
+
+606
+00:45:54,250 --> 00:46:00,410
+بعض بعدين بعقم اللوب بقلب البلاد 90 درجة باخد من
+
+607
+00:46:00,410 --> 00:46:05,670
+الربع الأول و بطلع بعدها بقلب 90 درجة و طبعا بعقم
+
+608
+00:46:05,670 --> 00:46:11,290
+اللوب باخد من الربع التاني و بطلع بقلب 90 درجة و
+
+609
+00:46:11,290 --> 00:46:15,810
+أكيد بعقم اللوب و باخد من الربع التالت و بعمل deep
+
+610
+00:46:15,810 --> 00:46:16,210
+bag
+
+611
+00:46:20,320 --> 00:46:24,160
+في عندي تلت طرق لزراعة الأجار إبلاد أول طريقة
+
+612
+00:46:24,160 --> 00:46:26,400
+تعرفنا عليها بالـ slide الأقبل اللي هي الـ
+
+613
+00:46:26,400 --> 00:46:30,480
+Quadrant String هاي الطريقة بستخدمها لحصول على
+
+614
+00:46:30,480 --> 00:46:34,360
+إيزولات كولوني لعمل بيورت كالتشار عشان أتعرف على
+
+615
+00:46:34,360 --> 00:46:38,760
+البكتيريا الطريقة التانية الـ Radiant String اللي
+
+616
+00:46:38,760 --> 00:46:43,860
+هي الشعاعي بستخدمها حتى أحصل أيضا على إيزولات
+
+617
+00:46:43,860 --> 00:46:47,680
+كولوني كفي طريقة الزراعة بزرعالربع الأول بشكل
+
+618
+00:46:47,680 --> 00:46:53,820
+continuous بعدين بطلع شعاع من الربع الأول بين كل
+
+619
+00:46:53,820 --> 00:46:58,660
+شعاع و التاني بعقن بعدين بقلب 90 درجة و بحاول أني
+
+620
+00:46:58,660 --> 00:47:03,320
+انا أطلع شعاع و بعقن بين كل شعاع و التاني هذه
+
+621
+00:47:03,320 --> 00:47:07,240
+الـVariant Structure الطريقة التالتة ال continuous
+
+622
+00:47:07,240 --> 00:47:12,200
+أو الـ Z-zag هذه الطريقة بنستخدمها لفحص المضادات
+
+623
+00:47:12,200 --> 00:47:18,120
+الحيوية و كمان بنستخدمها ل ال countطبعا بفتح أنا
+
+624
+00:47:18,120 --> 00:47:22,720
+الطبق بزرع بشكل continuous لازم يكون نتح كل الطبق
+
+625
+00:47:22,720 --> 00:47:32,300
+بالبكتيريا هيك تسمو كل البكتيريا على الطبق هاي
+
+626
+00:47:32,300 --> 00:47:36,500
+الطريقة بستخدمها في ال urine culture فنزل خط بعدين
+
+627
+00:47:36,500 --> 00:47:41,720
+بزرع بشكل continuous بالنسبة للثورة high الثورة
+
+628
+00:47:41,720 --> 00:47:46,910
+high الطبق مزروع بطريقة radiant spreadلكن هذه
+
+629
+00:47:46,910 --> 00:47:51,930
+الصورة مزروعة بطريقة الـ Quadrant Effect بحصل على
+
+630
+00:47:51,930 --> 00:47:57,050
+إيزولاتت كولونية على مستعمرات مفصولة عن بعض بقدر
+
+631
+00:47:57,050 --> 00:48:01,490
+أعمل منها view of culture و أتعرف على البكتيريا
+
+632
+00:48:01,490 --> 00:48:06,610
+خلصنا الشطر ياتيكم العافية جميعا والسلام عليكم
+
+633
+00:48:06,610 --> 00:48:08,250
+ورحمة الله وبركاته
+
diff --git a/PL9fwy3NUQKwZWgAb_V1ATlRRsH-6i3cPC/bxthOW-TT6Y_raw.json b/PL9fwy3NUQKwZWgAb_V1ATlRRsH-6i3cPC/bxthOW-TT6Y_raw.json
new file mode 100644
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Aseptic technique هي كل الإجراءات اللي بيتم اتخاذها لمنع الكونتا ماناشا for the most part بكتيريا وفيزيولوجيا", "tokens": [3555, 39648, 4117, 36145, 8032, 47302, 33688, 7435, 2288, 16606, 9307, 13672, 1829, 4724, 41193, 27842, 8978, 11296, 36145, 2423, 2407, 9381, 5172, 19446, 7251, 9381, 13546, 8978, 3224, 6055, 1211, 2407, 12984, 19446, 7251, 9381, 13546, 9122, 11536, 2655, 995, 3714, 7649, 33599, 995, 4724, 38251, 1829, 11296, 2423, 39184, 1018, 5250, 299, 6532, 7251, 3555, 4587, 7578, 2423, 39184, 1018, 5250, 299, 6532, 39896, 28242, 33688, 7435, 2288, 16606, 9307, 13672, 1829, 4724, 36081, 2304, 1975, 2655, 9778, 45636, 11296, 32767, 1863, 3615, 2423, 30544, 2655, 995, 3714, 7649, 33599, 995, 337, 264, 881, 644, 4724, 4117, 2655, 13546, 25528, 4032, 41185, 11622, 1829, 12610, 29245, 25528], "avg_logprob": -0.18906249769709327, "compression_ratio": 1.8585365853658538, "no_speech_prob": 0.0, "words": [{"start": 274.08, "end": 274.74, "word": "بقولك", "probability": 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{"start": 279.32, "end": 279.68, "word": " ما", "probability": 0.90087890625}, {"start": 279.68, "end": 280.04, "word": " يصير", "probability": 0.8346354166666666}, {"start": 280.04, "end": 280.54, "word": " كونتا", "probability": 0.52294921875}, {"start": 280.54, "end": 281.02, "word": " ماناشا", "probability": 0.7591552734375}, {"start": 281.02, "end": 281.88, "word": " بسميها", "probability": 0.9373779296875}, {"start": 281.88, "end": 282.58, "word": " الـ", "probability": 0.857666015625}, {"start": 282.58, "end": 283.22, "word": " Aseptic", "probability": 0.7747395833333334}, {"start": 283.22, "end": 283.78, "word": " technique", "probability": 0.48388671875}, {"start": 283.78, "end": 284.12, "word": " يبقى", "probability": 0.948974609375}, {"start": 284.12, "end": 284.3, "word": " الـ", "probability": 0.846923828125}, {"start": 284.3, "end": 284.72, "word": " Aseptic", "probability": 0.9034830729166666}, {"start": 284.72, "end": 285.24, "word": " technique", "probability": 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useless يبقى صار اندي mixed population يبقى ال culture بتصير useless غير صالحة للاستخدام for this reason اعتبرتك", "tokens": [2407, 9957, 4587, 38251, 4032, 1829, 9381, 13546, 8978, 11296, 16472, 16254, 9122, 11536, 46811, 7649, 33599, 7251, 3555, 4587, 7578, 2423, 3713, 8032, 1829, 9381, 13546, 7467, 4415, 37893, 6075, 3713, 257, 2167, 33745, 18932, 370, 393, 360, 264, 912, 551, 294, 257, 5911, 6075, 3713, 1455, 264, 3713, 14115, 7251, 3555, 4587, 7578, 20328, 9640, 16472, 16254, 7467, 4415, 7251, 3555, 4587, 7578, 2423, 3713, 39894, 9381, 13546, 14115, 32771, 13546, 20328, 6027, 5016, 3660, 24976, 995, 14851, 9778, 3215, 10943, 337, 341, 1778, 1975, 34268, 26890, 2655, 4117], "avg_logprob": -0.2345448367945526, "compression_ratio": 1.6652360515021458, "no_speech_prob": 4.947185516357422e-06, "words": [{"start": 376.19, "end": 376.99, "word": "وينقسم", "probability": 0.881103515625}, {"start": 376.99, "end": 377.49, "word": " ويصير", "probability": 0.84716796875}, {"start": 377.49, 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الوسط الغذائي لنمو البكتيريا in working with microorganism we must also have a sterile nutrient containing media in which to grow the organism طبعا ال media لازم تكون استيرال لازم تكون معقمة وهذا من ال aseptic technique anything in or on which we grow a microorganism is termed", "tokens": [3555, 38251, 1829, 23758, 2423, 41779, 9566, 2423, 3021, 2423, 3021, 39896, 2423, 41779, 9566, 6024, 118, 8848, 16373, 1829, 5296, 1863, 2304, 2407, 29739, 4117, 2655, 13546, 25528, 294, 1364, 365, 49129, 1434, 321, 1633, 611, 362, 257, 18924, 794, 32694, 19273, 3021, 294, 597, 281, 1852, 264, 24128, 23032, 3555, 3615, 995, 2423, 3021, 5296, 31377, 2304, 6055, 30544, 44713, 13546, 6027, 5296, 31377, 2304, 6055, 30544, 20449, 4587, 46007, 37037, 15730, 9154, 2423, 382, 5250, 299, 6532, 1340, 294, 420, 322, 597, 321, 1852, 257, 49129, 1434, 307, 1433, 292], "avg_logprob": -0.12915557987512427, "compression_ratio": 1.6514522821576763, "no_speech_prob": 0.0, "words": [{"start": 433.25, "end": 433.91, 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عشان ما أقتل ال iniculum ال iniculum المسح اللي أنا أخدتها", "tokens": [1211, 15042, 6055, 2655, 2407, 3224, 7435, 17513, 7251, 9381, 13546, 5296, 11536, 3224, 5551, 5016, 29973, 39896, 4117, 4724, 1829, 30544, 46811, 11933, 3555, 2655, 3615, 995, 4587, 32640, 264, 7198, 820, 312, 4350, 281, 1627, 12174, 12610, 1829, 39182, 19446, 46811, 6225, 42213, 10632, 16712, 3615, 4587, 32640, 4724, 14851, 1863, 7578, 32771, 1211, 4587, 13672, 37746, 34051, 2423, 11037, 36145, 11296, 6055, 26890, 3215, 32767, 41891, 6225, 46309, 5296, 3615, 46309, 9957, 1150, 12174, 36150, 19446, 41850, 5551, 14851, 9778, 40448, 11296, 983, 281, 5042, 8011, 264, 294, 299, 11560, 6225, 8592, 7649, 19446, 5551, 38149, 1211, 2423, 294, 299, 11560, 2423, 294, 299, 11560, 9673, 3794, 5016, 13672, 1829, 41850, 5551, 9778, 3215, 2655, 11296], "avg_logprob": -0.21796875732640425, "compression_ratio": 1.6591760299625469, "no_speech_prob": 0.0, "words": [{"start": 2116.9, "end": 2117.32, "word": "لما", "probability": 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ال", "probability": 0.98876953125}, {"start": 2140.8, "end": 2141.3, "word": " iniculum", "probability": 0.8135579427083334}, {"start": 2141.3, "end": 2141.48, "word": " ال", "probability": 0.88134765625}, {"start": 2141.48, "end": 2141.92, "word": " iniculum", "probability": 0.9138997395833334}, {"start": 2141.92, "end": 2142.4, "word": " المسح", "probability": 0.76416015625}, {"start": 2142.4, "end": 2142.56, "word": " اللي", "probability": 0.9501953125}, {"start": 2142.56, "end": 2142.74, "word": " أنا", "probability": 0.818359375}, {"start": 2142.74, "end": 2143.26, "word": " أخدتها", "probability": 0.9716796875}], "temperature": 1.0}, {"id": 78, "seek": 217089, "start": 2143.49, "end": 2170.89, "text": "in this way all contaminants on the wire are incinerated في هذه الطريقة كل ال contaminants اللي على ال wire أو على ال loop بيصلوا incinerated فيها ملاحظات don't blow on the instrument to cool them ممنوع أني أنفخ على ال loop أو ال needle عشان أخليه يبرد ممنوع don't touch the instrument to a car to cool them ممنوع أعمل touch بالأجار عشان أخليه يبرد", "tokens": [259, 341, 636, 439, 27562, 1719, 322, 264, 6234, 366, 834, 4564, 770, 8978, 29538, 41950, 16572, 28671, 28242, 2423, 27562, 1719, 13672, 1829, 15844, 2423, 6234, 34051, 15844, 2423, 6367, 4724, 1829, 36520, 14407, 834, 4564, 770, 8978, 11296, 3714, 15040, 5016, 19913, 9307, 500, 380, 6327, 322, 264, 7198, 281, 1627, 552, 3714, 27842, 45367, 14739, 1829, 14739, 5172, 9778, 15844, 2423, 6367, 34051, 2423, 11037, 6225, 8592, 7649, 5551, 9778, 20292, 3224, 7251, 26890, 3215, 3714, 27842, 45367, 500, 380, 2557, 264, 7198, 281, 257, 1032, 281, 1627, 552, 3714, 27842, 45367, 5551, 25957, 1211, 2557, 20666, 10721, 7435, 9640, 6225, 8592, 7649, 5551, 9778, 20292, 3224, 7251, 26890, 3215], "avg_logprob": -0.1774945242101686, "compression_ratio": 1.875, "no_speech_prob": 2.205371856689453e-06, "words": [{"start": 2143.49, "end": 2143.73, "word": "in", "probability": 0.5263671875}, {"start": 2143.73, "end": 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"end": 2154.93, "word": " incinerated", "probability": 0.7578125}, {"start": 2154.93, "end": 2155.65, "word": " فيها", "probability": 0.92919921875}, {"start": 2155.65, "end": 2156.35, "word": " ملاحظات", "probability": 0.93994140625}, {"start": 2156.35, "end": 2156.65, "word": " don't", "probability": 0.752197265625}, {"start": 2156.65, "end": 2157.03, "word": " blow", "probability": 0.83349609375}, {"start": 2157.03, "end": 2157.33, "word": " on", "probability": 0.94091796875}, {"start": 2157.33, "end": 2157.47, "word": " the", "probability": 0.92626953125}, {"start": 2157.47, "end": 2157.95, "word": " instrument", "probability": 0.87890625}, {"start": 2157.95, "end": 2158.23, "word": " to", "probability": 0.95947265625}, {"start": 2158.23, "end": 2158.47, "word": " cool", "probability": 0.96875}, {"start": 2158.47, "end": 2158.69, "word": " them", "probability": 0.931640625}, {"start": 2158.69, "end": 2159.05, "word": " ممنوع", "probability": 0.8489583333333334}, {"start": 2159.05, "end": 2159.25, "word": " أني", "probability": 0.6043701171875}, {"start": 2159.25, "end": 2159.83, "word": " أنفخ", "probability": 0.7835286458333334}, {"start": 2159.83, "end": 2160.13, "word": " على", "probability": 0.92919921875}, {"start": 2160.13, "end": 2160.73, "word": " ال", "probability": 0.91455078125}, {"start": 2160.73, "end": 2160.95, "word": " loop", "probability": 0.97509765625}, {"start": 2160.95, "end": 2161.21, "word": " أو", "probability": 0.8876953125}, {"start": 2161.21, "end": 2161.35, "word": " ال", "probability": 0.95703125}, {"start": 2161.35, "end": 2161.61, "word": " needle", "probability": 0.9736328125}, {"start": 2161.61, "end": 2162.23, "word": " عشان", "probability": 0.9620768229166666}, {"start": 2162.23, "end": 2163.11, "word": " أخليه", "probability": 0.78076171875}, {"start": 2163.11, "end": 2163.61, "word": " يبرد", "probability": 0.9124348958333334}, {"start": 2163.61, "end": 2164.45, "word": " ممنوع", "probability": 0.9500325520833334}, {"start": 2164.45, "end": 2164.87, "word": " don't", "probability": 0.958740234375}, {"start": 2164.87, "end": 2165.13, "word": " touch", "probability": 0.92138671875}, {"start": 2165.13, "end": 2165.35, "word": " the", "probability": 0.93505859375}, {"start": 2165.35, "end": 2165.89, "word": " instrument", "probability": 0.859375}, {"start": 2165.89, "end": 2166.07, "word": " to", "probability": 0.499267578125}, {"start": 2166.07, "end": 2166.21, "word": " a", "probability": 0.892578125}, {"start": 2166.21, "end": 2166.45, "word": " car", "probability": 0.18408203125}, {"start": 2166.45, "end": 2166.77, "word": " to", "probability": 0.96533203125}, {"start": 2166.77, "end": 2167.11, "word": " cool", "probability": 0.966796875}, {"start": 2167.11, "end": 2167.57, "word": " them", "probability": 0.90283203125}, {"start": 2167.57, "end": 2168.37, "word": " ممنوع", "probability": 0.95361328125}, {"start": 2168.37, "end": 2168.71, "word": " أعمل", "probability": 0.92431640625}, {"start": 2168.71, "end": 2169.09, "word": " touch", "probability": 0.908203125}, {"start": 2169.09, "end": 2169.69, "word": " بالأجار", "probability": 0.9571533203125}, {"start": 2169.69, "end": 2170.03, "word": " عشان", "probability": 0.9912109375}, {"start": 2170.03, "end": 2170.55, "word": " أخليه", "probability": 0.9671630859375}, {"start": 2170.55, "end": 2170.89, "word": " يبرد", "probability": 0.9952799479166666}], "temperature": 1.0}, {"id": 79, "seek": 219105, "start": 2171.25, "end": 2191.05, "text": "Don't lay the loop down once it is sterilized ممنوع أخلي اللوب أو الـ needle بعد ما أعقمه أحطه على البرج لأنه بيصير contaminant procedure for aseptically transfer أول شي بدي أعقم اللوب", "tokens": [7450, 380, 2360, 264, 6367, 760, 1564, 309, 307, 41477, 1602, 3714, 27842, 45367, 5551, 9778, 20292, 13672, 37746, 34051, 2423, 39184, 11037, 39182, 19446, 5551, 3615, 4587, 2304, 3224, 5551, 5016, 9566, 3224, 15844, 2423, 26890, 7435, 5296, 33456, 3224, 4724, 1829, 9381, 13546, 27562, 394, 10747, 337, 382, 5250, 984, 5003, 5551, 12610, 44049, 4724, 16254, 5551, 3615, 4587, 2304, 13672, 37746], "avg_logprob": -0.2569711538461538, "compression_ratio": 1.3210526315789475, "no_speech_prob": 5.960464477539063e-08, "words": [{"start": 2171.25, "end": 2171.75, "word": "Don't", "probability": 0.55810546875}, {"start": 2171.75, "end": 2172.03, "word": " lay", "probability": 0.87353515625}, {"start": 2172.03, "end": 2172.27, "word": " the", "probability": 0.89990234375}, {"start": 2172.27, "end": 2172.47, "word": " loop", "probability": 0.84326171875}, {"start": 2172.47, "end": 2172.85, "word": " down", "probability": 0.83447265625}, {"start": 2172.85, "end": 2173.25, "word": " once", "probability": 0.8740234375}, {"start": 2173.25, "end": 2173.51, "word": " it", "probability": 0.951171875}, {"start": 2173.51, "end": 2173.77, "word": " is", "probability": 0.61962890625}, {"start": 2173.77, "end": 2174.75, "word": " sterilized", "probability": 0.913330078125}, {"start": 2174.75, "end": 2175.53, "word": " ممنوع", "probability": 0.892578125}, {"start": 2175.53, "end": 2176.13, "word": " أخلي", "probability": 0.7023111979166666}, {"start": 2176.13, "end": 2176.79, "word": " اللوب", "probability": 0.5899658203125}, {"start": 2176.79, "end": 2177.01, "word": " أو", "probability": 0.87744140625}, {"start": 2177.01, "end": 2177.17, "word": " الـ", "probability": 0.6612548828125}, {"start": 2177.17, "end": 2177.37, "word": " needle", "probability": 0.48876953125}, {"start": 2177.37, "end": 2177.67, "word": " بعد", "probability": 0.95849609375}, {"start": 2177.67, "end": 2177.87, "word": " ما", "probability": 0.76953125}, {"start": 2177.87, "end": 2178.33, "word": " أعقمه", "probability": 0.96044921875}, {"start": 2178.33, "end": 2178.85, "word": " أحطه", "probability": 0.9566650390625}, {"start": 2178.85, "end": 2179.09, "word": " على", "probability": 0.90478515625}, {"start": 2179.09, "end": 2179.65, "word": " البرج", "probability": 0.7123209635416666}, {"start": 2179.65, "end": 2180.05, "word": " لأنه", "probability": 0.9363606770833334}, {"start": 2180.05, "end": 2180.47, "word": " بيصير", "probability": 0.939697265625}, {"start": 2180.47, "end": 2181.77, "word": " contaminant", "probability": 0.527740478515625}, {"start": 2181.77, "end": 2187.01, "word": " procedure", "probability": 0.728515625}, {"start": 2187.01, "end": 2187.57, "word": " for", "probability": 0.9404296875}, {"start": 2187.57, "end": 2188.33, "word": " aseptically", "probability": 0.8977864583333334}, {"start": 2188.33, "end": 2188.93, "word": " transfer", "probability": 0.6123046875}, {"start": 2188.93, "end": 2189.53, "word": " أول", "probability": 0.871826171875}, {"start": 2189.53, "end": 2189.69, "word": " شي", "probability": 0.51025390625}, {"start": 2189.69, "end": 2189.93, "word": " بدي", "probability": 0.579345703125}, {"start": 2189.93, "end": 2190.49, "word": " أعقم", "probability": 0.981689453125}, {"start": 2190.49, "end": 2191.05, "word": " اللوب", "probability": 0.93017578125}], "temperature": 1.0}, {"id": 80, "seek": 221748, "start": 2191.42, "end": 2217.48, "text": "واللي تاني إشي بدون ما تحط اللوب على الـ bench بتنسكي الـ culture tube بتعقم الفوهة بتمريرها على اللهد بسرعة ممنوع أحط الغطاية على الـ bench بضلي مسكاها بإيدي الغطاية بنسكها بنفس الإيدي اللي ماسكا فيها اللوب تالت إشي بدخل اللوب في الـ tube و بذرع رابع إشي بعقم بعد ما أخلص الشغل بعقم الفوهة و بفكرها", "tokens": [2407, 6027, 20292, 6055, 7649, 1829, 11933, 8592, 1829, 47525, 11536, 19446, 6055, 5016, 9566, 13672, 37746, 15844, 2423, 39184, 10638, 39894, 1863, 3794, 4117, 1829, 2423, 39184, 3713, 9917, 39894, 3615, 4587, 2304, 27188, 2407, 3224, 3660, 39894, 29973, 13546, 11296, 15844, 13672, 3224, 3215, 4724, 3794, 2288, 27884, 3714, 27842, 45367, 5551, 5016, 9566, 6024, 118, 9566, 995, 10632, 15844, 2423, 39184, 10638, 4724, 11242, 20292, 47524, 4117, 995, 11296, 4724, 28814, 1829, 16254, 6024, 118, 9566, 995, 10632, 44945, 3794, 4117, 11296, 44945, 36178, 33688, 1829, 16254, 13672, 1829, 3714, 32277, 4117, 995, 8978, 11296, 13672, 37746, 6055, 6027, 2655, 11933, 8592, 1829, 47525, 9778, 1211, 13672, 37746, 8978, 2423, 39184, 9917, 4032, 4724, 8848, 2288, 3615, 12602, 16758, 3615, 11933, 8592, 1829, 45030, 4587, 2304, 39182, 19446, 5551, 9778, 1211, 9381, 25124, 17082, 1211, 45030, 4587, 2304, 27188, 2407, 3224, 3660, 4032, 4724, 5172, 37983, 11296], "avg_logprob": -0.18284353890166377, "compression_ratio": 2.0931174089068825, "no_speech_prob": 0.0, "words": [{"start": 2191.42, "end": 2191.86, "word": "واللي", "probability": 0.8346354166666666}, {"start": 2191.86, "end": 2192.18, "word": " تاني", "probability": 0.9694010416666666}, {"start": 2192.18, "end": 2192.36, "word": " إشي", "probability": 0.7347819010416666}, {"start": 2192.36, "end": 2192.74, "word": " بدون", "probability": 0.9736328125}, {"start": 2192.74, "end": 2192.86, "word": " ما", "probability": 0.66796875}, {"start": 2192.86, "end": 2193.22, "word": " تحط", "probability": 0.7801106770833334}, {"start": 2193.22, "end": 2193.54, "word": " اللوب", "probability": 0.91064453125}, {"start": 2193.54, "end": 2193.74, "word": " على", "probability": 0.7294921875}, {"start": 2193.74, "end": 2193.98, "word": " الـ", "probability": 0.77734375}, {"start": 2193.98, "end": 2194.22, "word": " bench", "probability": 0.365234375}, {"start": 2194.22, "end": 2194.94, "word": " بتنسكي", "probability": 0.79853515625}, {"start": 2194.94, "end": 2195.34, "word": " الـ", "probability": 0.57958984375}, {"start": 2195.34, "end": 2195.54, "word": " culture", "probability": 0.4638671875}, {"start": 2195.54, "end": 2196.12, "word": " tube", "probability": 0.89794921875}, {"start": 2196.12, "end": 2196.78, "word": " بتعقم", "probability": 0.89599609375}, {"start": 2196.78, "end": 2197.26, "word": " الفوهة", "probability": 0.78857421875}, {"start": 2197.26, "end": 2197.92, "word": " بتمريرها", "probability": 0.8553466796875}, {"start": 2197.92, "end": 2198.16, "word": " على", "probability": 0.8046875}, {"start": 2198.16, "end": 2198.5, "word": " اللهد", "probability": 0.4733072916666667}, {"start": 2198.5, "end": 2199.06, "word": " بسرعة", "probability": 0.9853515625}, {"start": 2199.06, "end": 2200.02, "word": " ممنوع", "probability": 0.9500325520833334}, {"start": 2200.02, "end": 2200.44, "word": " أحط", "probability": 0.9599609375}, {"start": 2200.44, "end": 2201.1, "word": " الغطاية", "probability": 0.885107421875}, {"start": 2201.1, "end": 2201.38, "word": " على", "probability": 0.8603515625}, {"start": 2201.38, "end": 2201.58, "word": " الـ", "probability": 0.86962890625}, {"start": 2201.58, "end": 2201.8, "word": " bench", "probability": 0.79345703125}, {"start": 2201.8, "end": 2202.26, "word": " بضلي", "probability": 0.78515625}, {"start": 2202.26, "end": 2202.8, "word": " مسكاها", "probability": 0.75250244140625}, {"start": 2202.8, "end": 2203.26, "word": " بإيدي", "probability": 0.970947265625}, {"start": 2203.26, "end": 2204.3, "word": " الغطاية", "probability": 0.9150390625}, {"start": 2204.3, "end": 2204.82, "word": " بنسكها", "probability": 0.9146728515625}, {"start": 2204.82, "end": 2205.22, "word": " بنفس", "probability": 0.88037109375}, {"start": 2205.22, "end": 2205.66, "word": " الإيدي", "probability": 0.87646484375}, {"start": 2205.66, "end": 2205.9, "word": " اللي", "probability": 0.94091796875}, {"start": 2205.9, "end": 2206.5, "word": " ماسكا", "probability": 0.55987548828125}, {"start": 2206.5, "end": 2206.94, "word": " فيها", "probability": 0.986328125}, {"start": 2206.94, "end": 2207.54, "word": " اللوب", "probability": 0.9716796875}, {"start": 2207.54, "end": 2208.58, "word": " تالت", "probability": 0.9627278645833334}, {"start": 2208.58, "end": 2209.0, "word": " إشي", "probability": 0.9871419270833334}, {"start": 2209.0, "end": 2209.96, "word": " بدخل", "probability": 0.8876953125}, {"start": 2209.96, "end": 2210.48, "word": " اللوب", "probability": 0.978271484375}, {"start": 2210.48, "end": 2210.78, 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"word": " بعقم", "probability": 0.9376627604166666}, {"start": 2215.9, "end": 2216.58, "word": " الفوهة", "probability": 0.9737548828125}, {"start": 2216.58, "end": 2216.7, "word": " و", "probability": 0.73974609375}, {"start": 2216.7, "end": 2217.48, "word": " بفكرها", "probability": 0.8699951171875}], "temperature": 1.0}, {"id": 81, "seek": 224746, "start": 2218.08, "end": 2247.46, "text": "خامس شاق بكرر نفس الخطوات في حال أنا ذراعت أكتر من الـ tube لما أخلص الشغل بعقم اللوب و بحطه على ال bench لو أنا فكت أني أنا ما عقمت، بعقم هان الخطوات في صور بعقم اللوب بتعريضه لأ اللهب لما يصير لونه أحمر يتوهجك وعرف تم تعقيمه", "tokens": [9778, 10943, 3794, 13412, 995, 4587, 4724, 37983, 2288, 8717, 36178, 33962, 9566, 2407, 9307, 8978, 11331, 6027, 41850, 29910, 23557, 34268, 5551, 4117, 2655, 2288, 9154, 2423, 39184, 9917, 5296, 15042, 5551, 9778, 1211, 9381, 25124, 17082, 1211, 45030, 4587, 2304, 13672, 37746, 4032, 4724, 5016, 9566, 3224, 15844, 2423, 10638, 45164, 41850, 6156, 4117, 2655, 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{"start": 2235.02, "end": 2235.52, "word": " بعقم", "probability": 0.7737630208333334}, {"start": 2235.52, "end": 2239.72, "word": " هان", "probability": 0.59619140625}, {"start": 2239.72, "end": 2240.42, "word": " الخطوات", "probability": 0.9691162109375}, {"start": 2240.42, "end": 2240.76, "word": " في", "probability": 0.9462890625}, {"start": 2240.76, "end": 2241.4, "word": " صور", "probability": 0.982666015625}, {"start": 2241.4, "end": 2242.38, "word": " بعقم", "probability": 0.931640625}, {"start": 2242.38, "end": 2242.76, "word": " اللوب", "probability": 0.979736328125}, {"start": 2242.76, "end": 2243.5, "word": " بتعريضه", "probability": 0.80966796875}, {"start": 2243.5, "end": 2243.76, "word": " لأ", "probability": 0.5908203125}, {"start": 2243.76, "end": 2244.22, "word": " اللهب", "probability": 0.5078938802083334}, {"start": 2244.22, "end": 2244.4, "word": " لما", "probability": 0.897705078125}, {"start": 2244.4, "end": 2244.7, "word": " يصير", "probability": 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هبط أول جزء بيكون dark zone of unburned gas هذه المنطقة ما بيصير فيها احتراف بعدين عندي ال orange zone of incomplete combustion بعدين بتيجي منطقة ال blue zone of partial combustion أخر شئ ال colorless zone of complete combustion كل ما طلعنا لفوق بيزيد ال oxygen بيصير sufficient combustion احتراف كامل للغاز", "tokens": [6027, 11622, 16373, 3215, 13672, 1829, 8032, 3555, 9566, 5551, 12610, 10874, 11622, 38207, 4724, 1829, 30544, 2877, 6668, 295, 517, 21763, 292, 4211, 29538, 9673, 1863, 9566, 28671, 19446, 4724, 1829, 9381, 13546, 8978, 11296, 1975, 33753, 23557, 5172, 39182, 9957, 18871, 16254, 2423, 7671, 6668, 295, 31709, 28121, 39182, 9957, 4724, 31371, 7435, 1829, 9154, 9566, 28671, 2423, 3344, 6668, 295, 14641, 28121, 5551, 34740, 13412, 19986, 2423, 2017, 1832, 6668, 295, 3566, 28121, 28242, 19446, 23032, 1211, 3615, 8315, 5296, 5172, 30543, 4724, 1829, 11622, 25708, 2423, 9169, 4724, 1829, 9381, 13546, 11563, 28121, 1975, 33753, 23557, 5172, 9122, 10943, 1211, 24976, 17082, 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hottest بتكون شديدة الحرارة لكن لل luminous flame بتكون هي المنطقة ال orange not very hot", "tokens": [5172, 3555, 6027, 2655, 6027, 1829, 39894, 30544, 13412, 16254, 41891, 21542, 2288, 9640, 3660, 32780, 7251, 3555, 4587, 7578, 13672, 1829, 47525, 8315, 8717, 3615, 28480, 3224, 14739, 16712, 3615, 4587, 25708, 4724, 1829, 5016, 36520, 43242, 9673, 1863, 9566, 28671, 6024, 110, 2288, 4587, 3224, 34051, 9673, 1863, 9566, 28671, 2423, 2017, 1832, 39894, 2304, 1829, 11296, 2107, 12, 75, 7112, 563, 13287, 39894, 30544, 32780, 39894, 30544, 13412, 16254, 41891, 21542, 2288, 9640, 3660, 44381, 24976, 32476, 563, 13287, 39894, 30544, 39896, 9673, 1863, 9566, 28671, 2423, 7671, 406, 588, 2368], "avg_logprob": -0.18388157361432125, "compression_ratio": 1.883248730964467, "no_speech_prob": 5.960464477539063e-08, "words": [{"start": 2305.7, "end": 2306.4, "word": "فبالتالي", "probability": 0.9613444010416666}, {"start": 2306.4, "end": 2306.8, "word": " بتكون", "probability": 0.759521484375}, 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"probability": 0.86376953125}, {"start": 2354.62, "end": 2354.76, "word": " ال", "probability": 0.86376953125}, {"start": 2354.76, "end": 2355.42, "word": " incubator", "probability": 0.890869140625}, {"start": 2355.42, "end": 2355.54, "word": " و", "probability": 0.95458984375}, {"start": 2355.54, "end": 2355.94, "word": " عملت", "probability": 0.8310546875}, {"start": 2355.94, "end": 2356.82, "word": " incubation", "probability": 0.96826171875}, {"start": 2356.82, "end": 2357.04, "word": " يبقى", "probability": 0.958984375}, {"start": 2357.04, "end": 2357.5, "word": " المرحلة", "probability": 0.98486328125}, {"start": 2357.5, "end": 2357.9, "word": " التانية", "probability": 0.9627278645833334}, {"start": 2357.9, "end": 2358.76, "word": " incubation", "probability": 0.932861328125}], "temperature": 1.0}, {"id": 86, "seek": 238594, "start": 2359.04, "end": 2385.94, "text": "مدت 24 ساعة و أحيانا 48 حسب البكتيريا بعد الـ incubation هلاقي نمو للبكتيريا ممكن ينمو أكتر من نوع من البكتيريا 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colony أصلها خلية بكتيرية واحدة كيف بدي أحصل على ال isolated colony؟ to obtain well isolated colony it is essential to disperse the inoculum or sample on the surface of an enriched agar plate", "tokens": [47, 540, 3713, 3410, 281, 5876, 11763, 294, 257, 9115, 6889, 2644, 312, 1096, 5969, 14621, 23028, 366, 1143, 6225, 8592, 7649, 5551, 2655, 3615, 28480, 15844, 29739, 4117, 2655, 13546, 25528, 5296, 31377, 2304, 7251, 30544, 18871, 16254, 14621, 23028, 2423, 14621, 23028, 5551, 36520, 11296, 16490, 1211, 10632, 4724, 4117, 2655, 13546, 10632, 36764, 24401, 3660, 9122, 33911, 4724, 16254, 5551, 5016, 36520, 15844, 2423, 14621, 23028, 22807, 281, 12701, 731, 14621, 23028, 309, 307, 7115, 281, 717, 610, 405, 264, 294, 905, 11560, 420, 6889, 322, 264, 3753, 295, 364, 48624, 623, 289, 5924], "avg_logprob": -0.17586096604259646, "compression_ratio": 1.6455223880597014, "no_speech_prob": 1.9669532775878906e-06, "words": [{"start": 2389.79, "end": 2390.31, "word": "Pure", "probability": 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0.9573567708333334}, {"start": 2877.05, "end": 2877.51, "word": " أعمل", "probability": 0.94287109375}, {"start": 2877.51, "end": 2877.99, "word": " منها", "probability": 0.990234375}, {"start": 2877.99, "end": 2878.33, "word": " view", "probability": 0.061737060546875}, {"start": 2878.33, "end": 2878.61, "word": " of", "probability": 0.51611328125}, {"start": 2878.61, "end": 2879.01, "word": " culture", "probability": 0.8408203125}, {"start": 2879.01, "end": 2879.19, "word": " و", "probability": 0.53955078125}, {"start": 2879.19, "end": 2879.71, "word": " أتعرف", "probability": 0.9405517578125}, {"start": 2879.71, "end": 2880.03, "word": " على", "probability": 0.9423828125}, {"start": 2880.03, "end": 2881.49, "word": " البكتيريا", "probability": 0.9330078125}, {"start": 2881.49, "end": 2883.39, "word": " خلصنا", "probability": 0.947998046875}, {"start": 2883.39, "end": 2884.13, "word": " الشطر", "probability": 0.7950032552083334}, {"start": 2884.13, "end": 2884.59, "word": " ياتيكم", "probability": 0.766357421875}, {"start": 2884.59, "end": 2885.01, "word": " العافية", "probability": 0.9640299479166666}, {"start": 2885.01, "end": 2885.59, "word": " جميعا", "probability": 0.9498291015625}, {"start": 2885.59, "end": 2886.07, "word": " والسلام", "probability": 0.8299153645833334}, {"start": 2886.07, "end": 2886.61, "word": " عليكم", "probability": 0.94482421875}, {"start": 2886.61, "end": 2887.07, "word": " ورحمة", "probability": 0.9677734375}, {"start": 2887.07, "end": 2887.45, "word": " الله", "probability": 0.9677734375}, {"start": 2887.45, "end": 2888.25, "word": " وبركاته", "probability": 0.98515625}], "temperature": 1.0}], "language": "ar", "language_probability": 1.0, "duration": 2890.2341875, "duration_after_vad": 2808.8574999999887}
\ No newline at end of file
diff --git a/PL9fwy3NUQKwZWgAb_V1ATlRRsH-6i3cPC/bxthOW-TT6Y_raw.srt b/PL9fwy3NUQKwZWgAb_V1ATlRRsH-6i3cPC/bxthOW-TT6Y_raw.srt
new file mode 100644
index 0000000000000000000000000000000000000000..e5c332fb7a76b3137c037c8bf6423a95c9f81e19
--- /dev/null
+++ b/PL9fwy3NUQKwZWgAb_V1ATlRRsH-6i3cPC/bxthOW-TT6Y_raw.srt
@@ -0,0 +1,2532 @@
+1
+00:00:02,680 --> 00:00:06,220
+السلام عليكم ورحمة الله وبركاته نستكمل معاكم
+
+2
+00:00:06,220 --> 00:00:09,560
+محاضرات مساق الـ Microbiology العملية في هذا
+
+3
+00:00:09,560 --> 00:00:12,720
+اللقاء هنتكلم عن Media Preparation and
+
+4
+00:00:12,720 --> 00:00:16,060
+Sterilization Aseptic Techniques Pure Culture
+
+5
+00:00:16,060 --> 00:00:20,220
+Concept هنتعرف على طريق تحضير الـ Media لـ
+
+6
+00:00:20,220 --> 00:00:23,420
+Sterilization التعقيم كيف بدي أعقم الأدوات اللي
+
+7
+00:00:23,420 --> 00:00:26,940
+بستخدمها في معمل الـ Microbiology طبعا الـ
+
+8
+00:00:26,940 --> 00:00:29,920
+Sterilization يعتبر من الـ Aseptic Technique الـ
+
+9
+00:00:29,920 --> 00:00:33,570
+Aseptic Technique هي كل الإجراءاتاللي أنا بتبيعها
+
+10
+00:00:33,570 --> 00:00:37,690
+حتى أمنع ال contamination ال pure culture هي
+
+11
+00:00:37,690 --> 00:00:42,410
+المزرعة اللي بتكون المستعمرات الكولونية أصلها خلية
+
+12
+00:00:42,410 --> 00:00:46,210
+بكتيرية واحدة يعني بلاحظ على الطبق أن المستعمرة
+
+13
+00:00:46,210 --> 00:00:52,050
+كلها نفس ال morphology هذه المحاضرة هنتعرف على كل
+
+14
+00:00:52,050 --> 00:00:53,950
+من هذه المواضيع بالتفصيل
+
+15
+00:00:59,080 --> 00:01:02,480
+الـ Natural Environment Microorganism usually
+
+16
+00:01:02,480 --> 00:01:06,720
+exists as Mixed Population إيش يعني Mixed
+
+17
+00:01:06,720 --> 00:01:10,860
+Population لو أنا جبت السواب طبعا السواب بستخدمها
+
+18
+00:01:10,860 --> 00:01:15,580
+لأخذ مسحات أخدت بالسواب مسحة من الطاولة و زرعت
+
+19
+00:01:15,580 --> 00:01:19,280
+عالمي على وسط غذائي بعدين حطيت الطبق في ال
+
+20
+00:01:19,280 --> 00:01:23,700
+incubator لمدة 24 ساعة يعني عملت incubation ال
+
+21
+00:01:23,700 --> 00:01:26,960
+incubator طبعا هيوصل الظروف المناسبة لنمو
+
+22
+00:01:26,960 --> 00:01:32,030
+البكتيريابعد 24 ساعة هلاقي باندي فيه نمو على الطبق
+
+23
+00:01:32,030 --> 00:01:36,570
+هلاقي كولوني هذه الكولوني هتكون مختلفة في ال
+
+24
+00:01:36,570 --> 00:01:41,170
+morphology يعني هلاقي مجموعة من ال micro organism
+
+25
+00:01:41,170 --> 00:01:47,390
+mixed microorganism او mixed population وليس كائن
+
+26
+00:01:47,390 --> 00:01:50,770
+واحد however we are
+
+27
+00:01:58,510 --> 00:02:03,410
+عشان أدرس خصائص هذه الكائنات و أتعرف عليها لازم
+
+28
+00:02:03,410 --> 00:02:08,930
+أاخد كل كائن لحال لازم يكون عندي a pure culture شو
+
+29
+00:02:08,930 --> 00:02:13,270
+يعني a pure culture؟ a pure culture is one in
+
+30
+00:02:13,270 --> 00:02:17,430
+which all organisms are descendants of the same
+
+31
+00:02:17,430 --> 00:02:21,840
+organismهي المزرعة اللي بتكون المستعمرات عليها من
+
+32
+00:02:21,840 --> 00:02:27,540
+نفس السلالة يعني أصلها كائن واحد يعني أصلها خلية
+
+33
+00:02:27,540 --> 00:02:32,260
+بسكيرية واحدة technique for obtaining pure culture
+
+34
+00:02:32,260 --> 00:02:36,380
+from a mixed population will be described in this
+
+35
+00:02:36,380 --> 00:02:40,980
+slide لو أنا إجبتني عينيا natural وروحت زراعتها
+
+36
+00:02:40,980 --> 00:02:45,960
+بعد 24 ساعة لحظة عندي مستعمرات حجمها كبير
+
+37
+00:02:45,960 --> 00:02:49,610
+ومستعمرات حجمها صغيرةيبقى هان عندي mixed
+
+38
+00:02:49,610 --> 00:02:54,610
+population عشان أعمل identification لكل واحدة لازم
+
+39
+00:02:54,610 --> 00:02:59,310
+أعمل pure culture هذا المعمل هنتعرف كيف أنا بدي
+
+40
+00:02:59,310 --> 00:03:03,530
+أعمل pure culture عشان أتعرف على كل بكتيريا علاحي
+
+41
+00:03:03,530 --> 00:03:07,510
+ده من ال mixed population اللي أنا زرعتها في الأول
+
+42
+00:03:13,340 --> 00:03:17,040
+In working with microorganism, we must have a
+
+43
+00:03:17,040 --> 00:03:20,980
+method of transferring a growing organism from a
+
+44
+00:03:20,980 --> 00:03:23,680
+pure culture to a sterile medium without
+
+45
+00:03:23,680 --> 00:03:28,460
+introducing any unwanted outside contaminants خلال
+
+46
+00:03:28,460 --> 00:03:32,160
+شغل في معمل الـ Microbiology لازم يكون في عندي طرق
+
+47
+00:03:32,160 --> 00:03:36,480
+و إجراءات لمنع ال contamination في حال نقلت ال
+
+48
+00:03:36,480 --> 00:03:41,040
+micro organism من pure culture ل sterile media
+
+49
+00:03:41,040 --> 00:03:45,220
+يعني لوحة تانيةلو كان في عندي pure culture على
+
+50
+00:03:45,220 --> 00:03:50,360
+سبيل المثال E.coli وبدي أزرعها على وصف ثاني لازم
+
+51
+00:03:50,360 --> 00:03:53,540
+ألتزم بكل الطرق والإجراءات لمنع ال contamination
+
+52
+00:03:53,540 --> 00:03:59,000
+خلال عملية النقل أو حتى لما بدي أنقل micro
+
+53
+00:03:59,000 --> 00:04:02,780
+organism من mixed population ل pure culture لازم
+
+54
+00:04:02,780 --> 00:04:06,880
+ألتزم بالإجراءات لمنع ال contamination من ضمن هذه
+
+55
+00:04:06,880 --> 00:04:09,640
+الإجراءات أني أنا أمسح ال bench بالكخول قبل ما
+
+56
+00:04:09,640 --> 00:04:14,500
+أبدأ الشغللو استخدمت نيكروم لوبي تم تعقيمه قبل ما
+
+57
+00:04:14,500 --> 00:04:19,280
+أنا أستخدمه أفتغل جانب اللهب كمان كل أدواتي اللي
+
+58
+00:04:19,280 --> 00:04:24,360
+بستخدمها تكون معقمة من لوب Media4Cep ألشاب ونتعرف
+
+59
+00:04:24,360 --> 00:04:28,160
+في هذه المحاضرة على طرق تعقيم كل أداة في معمل الـ
+
+60
+00:04:28,160 --> 00:04:32,460
+Microbiology this method of preventing unwanted
+
+61
+00:04:32,460 --> 00:04:36,820
+microorganismبقولك إن هاي الإجراءات اللي باطمن
+
+62
+00:04:36,820 --> 00:04:40,540
+فيها إن الوصف ما يصير فيه تلوث ما يصير كونتا
+
+63
+00:04:40,540 --> 00:04:44,300
+ماناشا بسميها الـ Aseptic technique يبقى الـ
+
+64
+00:04:44,300 --> 00:04:47,920
+Aseptic technique هي كل الإجراءات اللي بيتم
+
+65
+00:04:47,920 --> 00:04:56,540
+اتخاذها لمنع الكونتا ماناشا for
+
+66
+00:04:56,540 --> 00:05:00,420
+the most part بكتيريا وفيزيولوجياonly can be
+
+67
+00:05:00,420 --> 00:05:04,400
+studied in pure culture عشان أدرس حصائص البكتيريا
+
+68
+00:05:04,400 --> 00:05:08,940
+لازم يكون عندي pure culture مش mixed population
+
+69
+00:05:08,940 --> 00:05:13,140
+the best way to obtain a pure culture is to start
+
+70
+00:05:13,140 --> 00:05:17,380
+with a single bacterial cell عشان أحصل على pure
+
+71
+00:05:17,380 --> 00:05:22,450
+culture لازم أبدأ ب single bacterial cellماذا يعني
+
+72
+00:05:22,450 --> 00:05:25,910
+single bacterial cell يعني isolated colony لأن ال
+
+73
+00:05:25,910 --> 00:05:30,470
+isolated colony أصلها خلية بكتيرية واحدة فلما بدي
+
+74
+00:05:30,470 --> 00:05:33,870
+أعمل pure culture من mixed population بروح بزرع
+
+75
+00:05:33,870 --> 00:05:37,990
+بطريقة الأربع باخد ال isolated colony من الربع
+
+76
+00:05:37,990 --> 00:05:43,470
+الرابع هيك بضمن أن ال culture يكون pure لأن أصلها
+
+77
+00:05:43,470 --> 00:05:46,650
+ال isolated colony خلية بكتيرية واحدة
+
+78
+00:05:52,950 --> 00:05:57,970
+الخلية تنقسم بسرعة تنتج ملايين من الخلايا خلال 24
+
+79
+00:05:57,970 --> 00:06:03,550
+ساعة يبقى بعد 24 ساعة هلاقي عندي ملايين من الخلايا
+
+80
+00:06:03,550 --> 00:06:08,270
+البكتيرية طب لو أنا ما اتبعت الـ Aseptic Technique
+
+81
+00:06:08,270 --> 00:06:12,810
+وكان عندي Contaminant unwanted غير اللي أنا بدي
+
+82
+00:06:12,810 --> 00:06:17,490
+إيها هينمو الـ unwanted الـcontaminantوينقسم ويصير
+
+83
+00:06:17,490 --> 00:06:21,750
+فيها اندي كون تماناش يبقى ال culture هيصير mixed
+
+84
+00:06:21,750 --> 00:06:26,690
+population مش pure culture a single unwanted
+
+85
+00:06:26,690 --> 00:06:31,170
+continent so can do the same thing in a otherwise
+
+86
+00:06:31,170 --> 00:06:36,050
+pure culture making the culture useless يبقى صار
+
+87
+00:06:36,050 --> 00:06:39,470
+اندي mixed population يبقى ال culture بتصير
+
+88
+00:06:39,470 --> 00:06:44,750
+useless غير صالحة للاستخدام for this reason
+
+89
+00:06:44,750 --> 00:06:49,610
+اعتبرتكI guess the restricted trial procedure must
+
+90
+00:06:49,610 --> 00:06:53,410
+be used عشان أعمل identification لازم يكون عندي
+
+91
+00:06:53,410 --> 00:06:57,750
+pure culture وليس mixed population عشان هيك لازم
+
+92
+00:06:57,750 --> 00:07:02,030
+يكون فيه إجراءات صارمة عشان أمنع ال contamination
+
+93
+00:07:02,030 --> 00:07:06,650
+عشان
+
+94
+00:07:06,650 --> 00:07:10,870
+أزرع و أنمي ال micro organism لازم يكون عندي وسط
+
+95
+00:07:10,870 --> 00:07:16,630
+غذائي لنمو البكتيريابسمي هذا الوسط ال media ال
+
+96
+00:07:16,630 --> 00:07:20,870
+media هي الوسط الغذائي لنمو البكتيريا in working
+
+97
+00:07:20,870 --> 00:07:24,810
+with microorganism we must also have a sterile
+
+98
+00:07:24,810 --> 00:07:28,410
+nutrient containing media in which to grow the
+
+99
+00:07:28,410 --> 00:07:32,530
+organism طبعا ال media لازم تكون استيرال لازم تكون
+
+100
+00:07:32,530 --> 00:07:37,370
+معقمة وهذا من ال aseptic technique anything in or
+
+101
+00:07:37,370 --> 00:07:41,490
+on which we grow a microorganism is termeda medium
+
+102
+00:07:41,490 --> 00:07:45,670
+بقولي أي إشي بينمي ال micro organism بسميه medium
+
+103
+00:07:45,670 --> 00:07:50,890
+ال medium مفرد جمحا media a nutrient blend ال
+
+104
+00:07:50,890 --> 00:07:54,790
+blend يعني خليط used to support microbial growth
+
+105
+00:07:54,790 --> 00:07:59,670
+هي خليط من المواد الغذائية لنمو ال micro organism
+
+106
+00:07:59,670 --> 00:08:04,910
+dehydrate powder according to manufactural
+
+107
+00:08:04,910 --> 00:08:10,390
+instruction بقوليهذه الميديا بتكون باودر كيف انا
+
+108
+00:08:10,390 --> 00:08:15,630
+بدي احضرها بحلها بدستل واتر dehydrate يعني الباودر
+
+109
+00:08:15,630 --> 00:08:20,290
+بدي احلها بدستل واتر حسب التعليمات الموجودة على
+
+110
+00:08:20,290 --> 00:08:25,290
+البوتل على سبيل المثال New Zealand Agar بتيجي بوتل
+
+111
+00:08:25,290 --> 00:08:32,570
+مكتوب عليها كل 28 جرام بحلها بـ 1 لتر دستل واتر
+
+112
+00:08:32,570 --> 00:08:36,930
+طبعا حسب الكمية اللي انا بحضرها لو بدي احضر 500 مل
+
+113
+00:08:37,200 --> 00:08:41,800
+بحسب أكم من جرام بدّي من البودر وبحلها بـ 500 ملي
+
+114
+00:08:41,800 --> 00:08:47,920
+دستيرل واتر agar is dried hydrophilic jelly-like
+
+115
+00:08:47,920 --> 00:08:52,960
+substance بقولّي الأجار قلنا عبارة عن solidifying
+
+116
+00:08:52,960 --> 00:08:58,320
+agent هو مادة مجففة زي الـ jelly هيدروفليك محبل
+
+117
+00:08:58,320 --> 00:09:02,840
+الماء بيعطي طبعا ال media القوام الصلب عشان ما
+
+118
+00:09:02,840 --> 00:09:07,450
+يكون سائلextracted from various species of red
+
+119
+00:09:07,450 --> 00:09:11,270
+algae بقولك بيستخلص من كائنات مختلفة من التحالب
+
+120
+00:09:11,270 --> 00:09:16,210
+الحمراء it's used as solidifying agent قولنا هو
+
+121
+00:09:16,210 --> 00:09:21,530
+يعتبر solidifying agent عشان تصلب ال media الألجار
+
+122
+00:09:21,530 --> 00:09:27,170
+بيدوب في درجة حرارة 85 وبتصلب عند درجة حرارة 40
+
+123
+00:09:33,040 --> 00:09:38,300
+Gelatin is colorless and odorless substance شو هو
+
+124
+00:09:38,300 --> 00:09:42,140
+الجيلاتين؟ الجيلاتين مادة مايلها رائحة و مايلها
+
+125
+00:09:42,140 --> 00:09:46,080
+لون طيب شو علاقتها بالميديا؟ قولنا زمان كانوا
+
+126
+00:09:46,080 --> 00:09:50,580
+يستخدموه كـ predefine agent لكن حاليا ما بيتم
+
+127
+00:09:50,580 --> 00:09:54,820
+استخدامه تم استبداله بالأجار طيب ليش تم استبداله و
+
+128
+00:09:54,820 --> 00:09:58,860
+ما بيتم استخدامه حاليا؟ لأن في بعض أنواع البكتيريا
+
+129
+00:09:58,860 --> 00:10:03,960
+بتفرز أنزيم الجيلاتينمن اسمه بيكسر الجلدين
+
+130
+00:10:03,960 --> 00:10:08,680
+فبالتالي بيتحول الوسط لثائق that is made from the
+
+131
+00:10:08,680 --> 00:10:13,240
+collagen مصنوع من الكلاجن بيدوب عن درجة حرارة 35
+
+132
+00:10:13,240 --> 00:10:19,520
+ألف في ال device وبيتطلب عن درجات حرارة أقل micro
+
+133
+00:10:19,520 --> 00:10:23,000
+organism are grown and subculture on different
+
+134
+00:10:23,000 --> 00:10:26,440
+type of chemical media بقول إن ال micro organism
+
+135
+00:10:26,440 --> 00:10:30,960
+بنميها و بعملها subculture على أوصات غذائية مختلفة
+
+136
+00:10:31,600 --> 00:10:35,560
+sub culture شو يعني sub culture؟ وشو الفرق بينها
+
+137
+00:10:35,560 --> 00:10:40,200
+وبين ال culture؟ لو أنا أخدت عينة ديوران وزرعتها
+
+138
+00:10:40,200 --> 00:10:44,640
+على وسط بحكي culture لكن لو زرعتها كمان مرة على
+
+139
+00:10:44,640 --> 00:10:49,480
+طبق تاني بحكي sub culture ال media بقولي ممكن تكون
+
+140
+00:10:49,480 --> 00:10:52,420
+chemically defined شو يعني chemically defined؟
+
+141
+00:10:52,420 --> 00:10:57,100
+يعني non concentration مكونات ال media كميات
+
+142
+00:10:57,100 --> 00:11:02,440
+معروفةممكن تكون الـ Media كيميكا دي Undefined
+
+143
+00:11:02,440 --> 00:11:07,480
+النسب تبعتها مش معروفة Unknown proportion a medium
+
+144
+00:11:07,480 --> 00:11:12,700
+is sterilized بس يعني sterilization يعني living
+
+145
+00:11:12,700 --> 00:11:17,820
+organism removed الـ Media قولنا كل أدواتي بيتم
+
+146
+00:11:17,820 --> 00:11:21,240
+تعقيمها والـ Media لازم اني انا اعقمها من الـ
+
+147
+00:11:21,240 --> 00:11:26,280
+Aseptic technique اني انا اعقمهاطرق تعقيم الـ
+
+148
+00:11:26,280 --> 00:11:29,700
+Media طبعا فين دي أكتر من طريقة لتعقيم الـ Media
+
+149
+00:11:29,700 --> 00:11:33,340
+قبل ما نتكلم عن طرق تعقيم الـ Media بنتكلم كيف أنا
+
+150
+00:11:33,340 --> 00:11:39,600
+بدي أحضر Media لو بدي أحضر الـ Nutrient Agar بقرأ
+
+151
+00:11:39,600 --> 00:11:42,780
+طبعا التعليمات الموجودة على الـ Bottle بتيجي
+
+152
+00:11:42,780 --> 00:11:45,920
+Bottle مكتوب عليها Nutrient Agar هاي الـ Bottle
+
+153
+00:11:45,920 --> 00:11:50,900
+Powder بقرأ التعليمات بوظن حسب الكمية اللي أنا بدي
+
+154
+00:11:50,900 --> 00:11:55,920
+إياهابعد ما أوزن بحط الوزنة في فلوسك بضيف ضد ال
+
+155
+00:11:55,920 --> 00:12:00,800
+water حسب الكمية و بحركها بعدين بحطها على ال hot
+
+156
+00:12:00,800 --> 00:12:05,420
+plate في زر لا الحرارة و زر لا السرعة طبعا قبلها
+
+157
+00:12:05,420 --> 00:12:09,780
+بنحط في الفلوسك مغناطيس حتى تدوب ال powder لما
+
+158
+00:12:09,780 --> 00:12:13,940
+تدوب منيح ال media بدخل الفلوسك على ال auto-clamp
+
+159
+00:12:13,940 --> 00:12:18,700
+ال auto-clamp هيعقم ليه ال media و يقتل كل ال
+
+160
+00:12:18,700 --> 00:12:25,150
+microorganism بما فيهملسباري هاك الـ media طلعت
+
+161
+00:12:25,150 --> 00:12:29,050
+معقّمة هاي من الـ Aseptic technique في طرق أخرى
+
+162
+00:12:29,050 --> 00:12:34,370
+منها الـ oven filtration UV exposure إيثالين 5 لكن
+
+163
+00:12:34,370 --> 00:12:38,070
+الـ most common اللي بيتم استخدامها الـ auto
+
+164
+00:12:38,070 --> 00:12:44,430
+-eclipse Other sterilization method طرق أخرى
+
+165
+00:12:44,430 --> 00:12:48,990
+للتعقيم إفلام انتجر عشان التوحشChemicals المواد
+
+166
+00:12:48,990 --> 00:12:52,270
+الكيميائية Ozone نيتروجين ديوكسايد هيدروجين
+
+167
+00:12:52,270 --> 00:12:55,770
+دروكسايد هذا الـ autoplayer used for sterilization
+
+168
+00:12:55,770 --> 00:13:01,110
+بعقم من خلاله الـ media بيتم قتل كل ال micro
+
+169
+00:13:01,110 --> 00:13:06,010
+organism بما فيهم الصغار تحت درجة حرارة عالية وضغط
+
+170
+00:13:06,010 --> 00:13:10,730
+مرتفع هذا هو الـ UV exposure الـ UV بتعمل على قتل
+
+171
+00:13:10,730 --> 00:13:14,990
+ال micro organism بالنسبة لهذه الطريقة هي ال
+
+172
+00:13:14,990 --> 00:13:19,420
+filtrationبكون محضرة ميديا و بحطها بـ cup و بسحب
+
+173
+00:13:19,420 --> 00:13:23,820
+الميديا بالسرنجة و بروح بلزق filter membrane زي
+
+174
+00:13:23,820 --> 00:13:27,940
+اللي بالصورة هي ال filter membrane بالسرنجة و بنزل
+
+175
+00:13:27,940 --> 00:13:32,780
+شويه شويه هيك بتتفلتر الميديا و أكيد بنزلها في الـ
+
+176
+00:13:32,780 --> 00:13:37,920
+sterile cup من الأمثلة على الميديا اللي أنا بستخدم
+
+177
+00:13:37,920 --> 00:13:42,660
+طريقة ال filtration لتحقيمها اليوريا أجار اليوريا
+
+178
+00:13:42,660 --> 00:13:47,780
+أجاربيتم تعقيمها باستخدام ال filtration لأن لو
+
+179
+00:13:47,780 --> 00:13:52,600
+دخلت ال auto-clamp بتتكسر هذه ال media فبستخدم
+
+180
+00:13:52,600 --> 00:13:57,700
+طريقة ال filtration هاي ال ethylene oxide وهذا هو
+
+181
+00:13:57,700 --> 00:14:03,780
+ال offer auto
+
+182
+00:14:03,780 --> 00:14:07,220
+-clamp the principle of sterilization in an auto
+
+183
+00:14:07,220 --> 00:14:12,010
+-clamp is that steam under pressure قولي إنهمبدأ
+
+184
+00:14:12,010 --> 00:14:15,630
+عمل الـ Autoclaval Sterilization steam under
+
+185
+00:14:15,630 --> 00:14:21,990
+pressure بيرفع درجة الحرارة لـ 121 بالتدريج لما
+
+186
+00:14:21,990 --> 00:14:28,710
+تصل لـ 121 بيبدأ يحسب الـ 15 minutes درجة حرارة
+
+187
+00:14:28,710 --> 00:14:33,050
+عالية 121 مع ضغط مرتفع كل ال micro organism بتموت
+
+188
+00:14:33,050 --> 00:14:37,250
+بما فيهم بسبع بعد ما تخلص الـ 15 minutes تنخسر
+
+189
+00:14:37,250 --> 00:14:41,580
+درجة الحرارة بالتدريجهك الميديا صارت استيرايل
+
+190
+00:14:41,580 --> 00:14:47,420
+معظمة بطلع الميديا بستناها تبرد شوية أقدر أني أنا
+
+191
+00:14:47,420 --> 00:14:51,760
+أمسكها بصوبها في petri dish جنب اللهب طبعا لازم
+
+192
+00:14:51,760 --> 00:14:54,420
+جنب اللهب لأنه من الـ Aseptic technique
+
+193
+00:14:54,420 --> 00:14:59,540
+sterilization of equipment and material wire loop
+
+194
+00:14:59,540 --> 00:15:03,660
+اللي هو الانيكلاتينج loop or iniculating needle
+
+195
+00:15:03,660 --> 00:15:09,010
+heat to redness inبزنسين بو عرنر إفلام كيف بدي أنا
+
+196
+00:15:09,010 --> 00:15:12,890
+أعقم الـ wire loop و اللي بحطه على الإفلام لما
+
+197
+00:15:12,890 --> 00:15:17,590
+يتوهج و يصير لونه أحمر هيك بتكون تم تعقيمه لـ
+
+198
+00:15:17,590 --> 00:15:22,890
+glassware الذجاجيات بيتم تعقيمها بالـ oven بالنسبة
+
+199
+00:15:22,890 --> 00:15:28,650
+للـ oven درجة الحرارة بتكون من 160 ل180 درجة
+
+200
+00:15:28,650 --> 00:15:33,310
+سليسيا لمدة ساعتين لـ glassware أنا لما أدخلها على
+
+201
+00:15:33,310 --> 00:15:38,530
+ال ovenبلفها بيه سلفان او ورق زي السلفان ده يسميه
+
+202
+00:15:38,530 --> 00:15:43,090
+grass proof paper او aluminium allowing additional
+
+203
+00:15:43,090 --> 00:15:46,930
+time for items to come to temperature and cool
+
+204
+00:15:46,930 --> 00:15:53,070
+down وقوللي هان انه لما تصل درجة الحرارة حرارة ال
+
+205
+00:15:53,070 --> 00:15:58,770
+oven ل160 او 180 بيبدأ يحسب الساعتين لما اتخلص
+
+206
+00:15:58,770 --> 00:16:03,300
+الساعتين بتنزل درجة الحرارة وبيفتح الجهازبالنسبة
+
+207
+00:16:03,300 --> 00:16:08,840
+لـ Plastic بتريدش بتيجي من الشركة بارد بتكون معقمة
+
+208
+00:16:08,840 --> 00:16:13,580
+استيرايل Culture Media and Solutions Culture Media
+
+209
+00:16:13,580 --> 00:16:17,300
+قلنا بيتم تعقيمها في الـ Autoclave solution زي الـ
+
+210
+00:16:17,300 --> 00:16:20,460
+Normal Saline كذلك بيتم تعقيمه في الـ Autoclave
+
+211
+00:16:20,460 --> 00:16:23,580
+اللي هو الـ Pressure Cooker قلنا زي فنجار تفضغط
+
+212
+00:16:23,580 --> 00:16:27,880
+الـ Glass Breeder اللي هو الألشاب الـMetal Forceps
+
+213
+00:16:27,880 --> 00:16:32,190
+الـ Forceps بيتم استخدامه اللي هو الملقطفي فحص الـ
+
+214
+00:16:32,190 --> 00:16:37,430
+Antibiotics بحطه فيه كحوس بعدين بعرضه على إلهام
+
+215
+00:16:37,430 --> 00:16:45,130
+Flaming in Alcohol Sterile
+
+216
+00:16:45,130 --> 00:16:48,550
+Medium هي Medium لكن بعد ما طلعتها من الـ Auto
+
+217
+00:16:48,550 --> 00:16:53,550
+class تكون الوسط خالي من كل ال micro organism مافي
+
+218
+00:16:53,550 --> 00:16:57,230
+عندي living microorganism is one which is free of
+
+219
+00:16:57,230 --> 00:17:02,060
+all life formsIt is usually sterilized by heating
+
+220
+00:17:02,060 --> 00:17:05,480
+it to a temperature at which all contaminant
+
+221
+00:17:05,480 --> 00:17:09,060
+microorganisms are destroyed كل الميكروعجنزم راح
+
+222
+00:17:09,060 --> 00:17:13,880
+تتذمر في ما فيهم بسبوع Colony is the smallest
+
+223
+00:17:13,880 --> 00:17:18,720
+bacterial unit that can be seen with the naked eye
+
+224
+00:17:18,720 --> 00:17:22,220
+هي أصغر واحدة بكتيرية ممكن أنا أشوفها قولنا ال
+
+225
+00:17:22,220 --> 00:17:25,780
+isolated colony بحصل عليها من الربع الرابع لو أنا
+
+226
+00:17:25,780 --> 00:17:30,780
+زرعت بطريقة الأربع cultureلو إجتني عينة يورينز
+
+227
+00:17:30,780 --> 00:17:34,320
+زرعتها أول مرة بسميها culture يبقى هي جزء من
+
+228
+00:17:34,320 --> 00:17:38,880
+العينة is part of a specimen الـ ground بنميها in
+
+229
+00:17:38,880 --> 00:17:43,220
+cultural media بيتم زراعتها و تنميتها على cultural
+
+230
+00:17:43,220 --> 00:17:47,360
+media طيب شو تعريف الـ cultural media is a medium
+
+231
+00:17:47,360 --> 00:17:52,880
+هي وسط liquid أو ثائل that contain nutrients to
+
+232
+00:17:52,880 --> 00:17:57,120
+grow bacteria in vitro فيها مغذيات لازمة لنمو
+
+233
+00:17:57,120 --> 00:18:01,780
+البكتيرياليس لازم أزرع و أنمي البكتيريا فين دي
+
+234
+00:18:01,780 --> 00:18:05,860
+سببان الصف الأول sometimes we cannot identify with
+
+235
+00:18:05,860 --> 00:18:09,820
+microscopical examination directly لأنه بال direct
+
+236
+00:18:09,820 --> 00:18:13,540
+examination اسمه direct بجيب العينة اللي بدي
+
+237
+00:18:13,540 --> 00:18:17,860
+أبحثها على سبيل المثال عينة urine بcenterها باخد
+
+238
+00:18:17,860 --> 00:18:20,960
+ال sediments بشوف تحت ال microscope هذا اسمه
+
+239
+00:18:20,960 --> 00:18:24,800
+direct examination فقط هشوف عندي بكتيريا أو لأ
+
+240
+00:18:24,800 --> 00:18:29,440
+ممكن أتعرف على شكلهاأو على هل هي gram positive أو
+
+241
+00:18:29,440 --> 00:18:32,780
+gram negative من خلال صباغة ال slide لكن ما بقدر
+
+242
+00:18:32,780 --> 00:18:38,660
+أتعرف بشكل نهائي على البكتيريا لازم أعزلها وأنميها
+
+243
+00:18:38,660 --> 00:18:43,060
+وأجري عليها فحوصات كيميائية يتواصلنا للتعريف
+
+244
+00:18:43,060 --> 00:18:47,960
+النهائي سبب التاني sometimes we do culture for
+
+245
+00:18:47,960 --> 00:18:52,160
+antibiotic sensitivity تستهيب ان بعد ما أعرف أي
+
+246
+00:18:52,160 --> 00:18:57,490
+بكتيريا لازم أعمل فحص المضادات الحيوية عشان أشوفهي
+
+247
+00:18:57,490 --> 00:19:02,730
+البكتيريا لأي مبادح حيوي هتستجيب عشان الدكتور يوصف
+
+248
+00:19:02,730 --> 00:19:06,590
+العلاج للمريض في الفحص بعتمد أنه أنا بدي أجيب
+
+249
+00:19:06,590 --> 00:19:11,730
+البكتيريا اللي نميتها وعرفتها بزراها على طبق بشكل
+
+250
+00:19:11,730 --> 00:19:15,770
+continuous بدي أتأكد أن الطبق كله زرعت عليه
+
+251
+00:19:15,770 --> 00:19:20,870
+بكتيريا بعدها أحط قراس Antibiotic فوق البكتيريا
+
+252
+00:19:20,870 --> 00:19:25,070
+اللي أنا زرعتها بحطها في ال incubator بعد 24 ساعة
+
+253
+00:19:25,070 --> 00:19:30,090
+بشوف النتيجةإذا كانت البكتيريا حساسة لـ Antibiotic
+
+254
+00:19:30,090 --> 00:19:34,370
+بشوف zone of inhibition منطقة فاضية حوالين الـ
+
+255
+00:19:34,370 --> 00:19:37,770
+Antibiotic يعني الـ Antibiotic قتل هذه البكتيريا
+
+256
+00:19:37,770 --> 00:19:44,330
+لو كانت مقاومة للـ Antibiotic هشوف نمول البكتيريا
+
+257
+00:19:44,330 --> 00:19:48,210
+حوالين الـ Antibiotic أكيد الدكتور هيختار الـ
+
+258
+00:19:48,210 --> 00:19:52,870
+Antibiotic اللي كان طبعا أو البكتيريا اللي كانت
+
+259
+00:19:52,870 --> 00:19:57,770
+حساسة لـ Antibioticمش المقاومة لأنه مش هيستجيب له
+
+260
+00:19:57,770 --> 00:20:01,870
+خاطرات الـ Media أكيد الـ Media بتدعم نمو
+
+261
+00:20:01,870 --> 00:20:07,050
+البكتيريا بتحتوي على مواد غذائية الـ PF للميديا
+
+262
+00:20:07,050 --> 00:20:11,550
+بيكون neutral to slightly alkaline درجة الحرارة
+
+263
+00:20:11,550 --> 00:20:17,130
+لازم تكون مناسبة لأن البكتيريا بتنمو عن درجة حرارة
+
+264
+00:20:17,130 --> 00:20:17,450
+37
+
+265
+00:20:20,740 --> 00:20:25,080
+بشوف zone of inhibition حوالين ال antibiotic يعني
+
+266
+00:20:25,080 --> 00:20:29,960
+ال antibiotic قطل high البكتيريا high معناها ان هي
+
+267
+00:20:29,960 --> 00:20:34,840
+حساسة لكن لو كانت مقاومة ل antibiotic معين هشوف
+
+268
+00:20:34,840 --> 00:20:38,340
+نمو البكتيريا حوالين ال antibiotic هان في نمو
+
+269
+00:20:38,340 --> 00:20:41,620
+حوالين ال antibiotic يبقى مقاومة لكن هان zone of
+
+270
+00:20:41,620 --> 00:20:46,720
+inhibition يبقى حساسة فالدكتور رح يختارالـ
+
+271
+00:20:46,720 --> 00:20:52,720
+Antibiotic الحساس مش المقاوم لأنه مش هيستجيب إذن
+
+272
+00:20:52,720 --> 00:20:58,980
+ال
+
+273
+00:20:58,980 --> 00:21:02,200
+media بنصمسها بناء على تلت معايير حسب ال
+
+274
+00:21:02,200 --> 00:21:05,680
+consistency حسب القوام ال nutrition المواد
+
+275
+00:21:05,680 --> 00:21:09,660
+الغذائية ال function الوظيفة بالنسبة للقوام بصمسها
+
+276
+00:21:09,660 --> 00:21:15,930
+لسولد سيمي سولد براس سولد نسبة الأجار بتكونمن 1.5%
+
+277
+00:21:15,930 --> 00:21:20,510
+لـ 2% أجار من الأمثلة عليها لـ Nutrients أجار ال
+
+278
+00:21:20,510 --> 00:21:24,090
+chemical lead نسبة الأجار بتكون 4 من 10% من
+
+279
+00:21:24,090 --> 00:21:28,110
+الأمثلة عليها ال media SIM أو اللي استخدمناها في
+
+280
+00:21:28,110 --> 00:21:33,050
+فحص المجتلد ال broth ما فيها أجار، نسبة الأجار
+
+281
+00:21:33,050 --> 00:21:36,230
+Zero من الأمثلة عليها لـ Nutrients broth أو
+
+282
+00:21:36,230 --> 00:21:39,650
+النيترات البروث أو ال brain hard infusion البروث
+
+283
+00:21:40,640 --> 00:21:43,900
+تاني معيار حسب الـ Nutrition حسب المواد الغذائية
+
+284
+00:21:43,900 --> 00:21:48,780
+بصنفها إما لـ Simple أو Complex الـ Simple بتحتوي
+
+285
+00:21:48,780 --> 00:21:53,300
+على مغذي واحد فقط مصدر واحد للتغذية من الأمثلة
+
+286
+00:21:53,300 --> 00:21:57,040
+عليها الـ Beton Water Broth في هذه الميديا الـ
+
+287
+00:21:57,040 --> 00:22:01,400
+Beton هو مصدر التغذية الوحيد الـ Complex بكون فيه
+
+288
+00:22:01,400 --> 00:22:05,120
+أكثر من مصدر للتغذية من الأمثلة عليها الـ Blood
+
+289
+00:22:05,120 --> 00:22:09,570
+Agaricتالت ميار على حسب الـ Function تقسم إلى
+
+290
+00:22:09,570 --> 00:22:12,870
+General Selective Differential Transport
+
+291
+00:22:12,870 --> 00:22:17,710
+Enrichment General من اسمها General بتنمي معظم
+
+292
+00:22:17,710 --> 00:22:20,710
+أنواع البكتيريا نيوتريان أجار من الأمثلة عليها
+
+293
+00:22:20,710 --> 00:22:25,950
+Selective اختيارية بتنمي أنواع معينة من البكتيريا
+
+294
+00:22:25,950 --> 00:22:30,690
+متشابهة في الحصاء يعني حسب حصية معينة من الأمثلة
+
+295
+00:22:30,690 --> 00:22:35,340
+عليها الـ Manitoulin Source Agile MSAhigh الـ MSA
+
+296
+00:22:35,340 --> 00:22:38,760
+بتنمي فقط البكتيريا اللي بتتحمل درجة الملوحة
+
+297
+00:22:38,760 --> 00:22:41,980
+العالية الـ high concentration of salts يبقى
+
+298
+00:22:41,980 --> 00:22:47,000
+الخاصية هي تحمل درجة الملوحة الـ NACL الموجود في
+
+299
+00:22:47,000 --> 00:22:53,140
+الـ media تركيزه 7.5% أكيد بقى البكتيريا مارح
+
+300
+00:22:53,140 --> 00:22:56,700
+تتحمل هذه الدرجة العالية من الـ salt فبالتالي مارح
+
+301
+00:22:56,700 --> 00:23:01,440
+تلمس الـ differential من اسمها differential هتميز
+
+302
+00:23:01,440 --> 00:23:07,080
+أكتر من نوعمن الـ Bacteria بتعطي كل نوع لون معين
+
+303
+00:23:07,080 --> 00:23:10,060
+من الأمثلة عليها الـ Manifold false eggs الـ
+
+304
+00:23:10,060 --> 00:23:12,940
+Manifold false أجار تعتبر selective and
+
+305
+00:23:12,940 --> 00:23:17,300
+differential ال transport media ال transport media
+
+306
+00:23:17,300 --> 00:23:21,220
+من الأمثلة عليها ال securette securette transport
+
+307
+00:23:21,220 --> 00:23:27,200
+media هو عبارة عن أسواق فيها media هي تستخدم لو ما
+
+308
+00:23:27,200 --> 00:23:31,000
+كان ضمن العين بعيد عن المختبر أكيد بستخدم ال
+
+309
+00:23:31,000 --> 00:23:35,730
+transport mediaتحفظ البكتيريا عشان ما تموت خلال
+
+310
+00:23:35,730 --> 00:23:39,190
+عملية المقل قبل ما أنا أشتغلها لو أنا ماعندي
+
+311
+00:23:39,190 --> 00:23:42,550
+Transport Media ممكن أني أحطها في Normal Saline
+
+312
+00:23:42,550 --> 00:23:46,650
+الـ Normal Saline هيحفظ البكتيريا من إنها اتموت
+
+313
+00:23:46,650 --> 00:23:51,430
+خامس نوع اللي هو الـ Enrich الـ Enrich في أنها
+
+314
+00:23:51,430 --> 00:23:55,950
+تحتاج لبعض الإضافات بعد ما يتم تحضير الـ Media لأن
+
+315
+00:23:55,950 --> 00:24:00,370
+في بعض أنواع البكتيريا تعتبر fastidious مدلة لأ
+
+316
+00:24:00,370 --> 00:24:06,940
+عشان تنمو تحتاج إلىبعض الإضافات من الإضافات ممكن
+
+317
+00:24:06,940 --> 00:24:11,940
+نضيف blood ممكن نضيف egg ممكن نضيف serum بالنسبة
+
+318
+00:24:11,940 --> 00:24:16,200
+لأمثلة على ال enriched ال blood media او ال
+
+319
+00:24:16,200 --> 00:24:20,940
+chocolate media بالنسبة لل blood نضيف البلد لكن
+
+320
+00:24:20,940 --> 00:24:24,260
+البلد بعد ما انا اطلع طبعا هي هواكس بحد ال
+
+321
+00:24:24,260 --> 00:24:28,140
+nutrient agar بعد ما انا اطلعها من ال autoclave
+
+322
+00:24:28,140 --> 00:24:33,700
+بخليها تبرد بضيف البلد بعد ما تبردبنسبة خمسة في
+
+323
+00:24:33,700 --> 00:24:39,900
+المية يعني الخمسة بدها 100 مل الـ nutrient agar لو
+
+324
+00:24:39,900 --> 00:24:44,460
+أنا حضرت ألف ملي من الـ nutrient agar بحط خمسين مل
+
+325
+00:24:44,460 --> 00:24:49,360
+من الـ blood على الألف مل من الـ nutrient agar لكن
+
+326
+00:24:49,360 --> 00:24:54,720
+للشوكولات أنا بضيف الـ blood هو اللي بعد ما أطلع
+
+327
+00:24:54,720 --> 00:24:58,140
+ال media من الـ autoclave وهي سخنة ليش؟ لأن درجة
+
+328
+00:24:58,140 --> 00:25:02,200
+الحرارة العالية بتعمل cumulative لل RPTsبتطلع
+
+329
+00:25:02,200 --> 00:25:08,020
+فاكتور Exovacter في المهمين لنمو بعض أنواع
+
+330
+00:25:08,020 --> 00:25:12,240
+البكتيريا اللي هي الفاصلية البكتيريا ال enrichment
+
+331
+00:25:12,240 --> 00:25:17,060
+ال enrichment هي بتعمل inhibition لنمو ال unwanted
+
+332
+00:25:17,060 --> 00:25:21,520
+بكتيريا بالتالي بتسمح لنمو فقط ال wanted بكتيريا
+
+333
+00:25:21,520 --> 00:25:25,240
+أنها تنمو بكل أرياحية مثل الأمثلة عليها Selenite
+
+334
+00:25:25,240 --> 00:25:29,670
+of Fibrose بتنميالـ Salmonella الألكالاين بيبتون
+
+335
+00:25:29,670 --> 00:25:36,950
+واتر اللي بتنمي الـ Vibrio cholera هاي
+
+336
+00:25:36,950 --> 00:25:41,870
+هي ال transport media هي كبتيجي بكات بتكون معقمة
+
+337
+00:25:41,870 --> 00:25:46,570
+استيرال لو أنا بدي أخد مسحة باخد المسحة بالسواب
+
+338
+00:25:46,570 --> 00:25:51,210
+هذا بعد ما أخد المسحة بحطه في التيوب هذا اللي
+
+339
+00:25:51,210 --> 00:25:52,870
+موجود في الميديا
+
+340
+00:25:57,380 --> 00:26:01,020
+Forms of Culture Media أشكال الـ Culture Media أول
+
+341
+00:26:01,020 --> 00:26:05,580
+شكل البروث الـ Q الميديا ممكن تكون بروث طبعا
+
+342
+00:26:05,580 --> 00:26:10,280
+البروث بحطها بـ Q RQ's containing a liquid media
+
+343
+00:26:10,280 --> 00:26:14,700
+عنابيب تحتوي على liquid media على ميديا سائلة a
+
+344
+00:26:14,700 --> 00:26:19,580
+typical nutrient containing بروث ميديا تشقق سيبروث
+
+345
+00:26:19,580 --> 00:26:25,370
+اتنوثيانت بروث من الأمثلة على البروث الـ Qطيب بعد
+
+346
+00:26:25,370 --> 00:26:28,870
+ما أزرع البكتيريا في بروست يو كيف أعرف نمط
+
+347
+00:26:28,870 --> 00:26:33,330
+البكتيريا أو لأ و اللي بتلاقي انه موبى تلت أشكال
+
+348
+00:26:33,330 --> 00:26:37,930
+ممكن تلاحظي أزوان ممكن تلاقيه كشكل او a
+
+349
+00:26:37,930 --> 00:26:41,690
+combination of three forms أو تلاقيهم مستمعين
+
+350
+00:26:41,690 --> 00:26:48,310
+التلت أشكال نتعرف على التلت أشكال أول واحد البريكل
+
+351
+00:26:48,310 --> 00:26:52,170
+a mass of organism is floating on top of the
+
+352
+00:26:52,170 --> 00:26:57,500
+prostateبقوللي بتكون البكتيريا بتنمو على السطح
+
+353
+00:26:57,500 --> 00:27:02,620
+بتطفو على السطح turbidity the organism appear as a
+
+354
+00:27:02,620 --> 00:27:06,100
+general cloudiness throughout the breath بتكون
+
+355
+00:27:06,100 --> 00:27:11,360
+امعكزة لتيوب sediment a mass of organism كتلة من
+
+356
+00:27:11,360 --> 00:27:15,480
+الخلايا بتكون at the bottom of the tube بتكون
+
+357
+00:27:15,480 --> 00:27:20,870
+متركزة في القاع طبعا الاختلافحسب نوع البكتيريا
+
+358
+00:27:20,870 --> 00:27:25,330
+واختلاف احتياجاتها للأكسجين فمثلًا الـ pellicle
+
+359
+00:27:25,330 --> 00:27:29,950
+بتكون aerobic لكن ال sediment بتكون anaerobic
+
+360
+00:27:29,950 --> 00:27:35,730
+طريقة الزراعة باخذ ال-iniculum أو البكتيريا وبعمل
+
+361
+00:27:35,730 --> 00:27:37,350
+mix لبراس EQ
+
+362
+00:27:42,170 --> 00:27:46,390
+بالنسبة لأشكال انمو في البروس a tube لو كانت
+
+363
+00:27:46,390 --> 00:27:50,510
+مجموعة الخلايا مترسبة في القاع يبقى بتكون sediment
+
+364
+00:27:50,510 --> 00:27:54,270
+لو كانت طافية على السطح bellicle بنلاحظ بنفس ال
+
+365
+00:27:54,270 --> 00:27:58,650
+tube في عندي كمان مجموعة من الأورجانيزم مترسب في
+
+366
+00:27:58,650 --> 00:28:01,650
+القاع يبقى sediment في هاي ال tube في عندي
+
+367
+00:28:01,650 --> 00:28:05,010
+bellicle and sediment في ال slide القبل قلنا ممكن
+
+368
+00:28:05,010 --> 00:28:09,910
+ألاقي انمو بشكل أو بأكتر من شكل أغر شكل التربي دي
+
+369
+00:28:09,910 --> 00:28:14,230
+تيب كل tube امعكرخلصنا البروتاتيوب أو الشكل من
+
+370
+00:28:14,230 --> 00:28:18,110
+أشكال الـ culture ميدياني جلدان شكل الـ slant tube
+
+371
+00:28:18,110 --> 00:28:22,310
+are tubes containing a nutrient medium بلصق
+
+372
+00:28:22,310 --> 00:28:27,470
+صليدفاين أجار أنابيب تحتوي على الـ nutrient media
+
+373
+00:28:27,470 --> 00:28:33,130
+على وصف غذائي مع صليدفاين أجار في البروتاتيوب كان
+
+374
+00:28:33,130 --> 00:28:37,070
+في عندي وصف غذائي لكن ماكن في عندي صليدفاين أجار
+
+375
+00:28:37,070 --> 00:28:40,750
+ماكن في عندي أجار كانت في بروت ثائل هذه الفرق
+
+376
+00:28:40,750 --> 00:28:47,390
+بينها وبينبالنسبة للإسلانتي تيوب كيف احصل على شكل
+
+377
+00:28:47,390 --> 00:28:55,190
+الإسلان؟ شكل الإسلان بيكون هاك لو رسمناه هي التيوب
+
+378
+00:28:55,190 --> 00:29:02,690
+بيكون مائل كيف احصل على شكل الإسلان؟ بتركها تتصلب
+
+379
+00:29:02,690 --> 00:29:05,630
+في زاوية يعني بعد ما طلعها من الـ autoclave بحطها
+
+380
+00:29:05,630 --> 00:29:09,550
+في زاوية عشان تتصلب في زاوية كيف بصراع الإسلانتي
+
+381
+00:29:09,550 --> 00:29:14,870
+تيوب؟بدخل بعمل طعن للمنطقة اللي تحت بدخل بالـ
+
+382
+00:29:14,870 --> 00:29:18,750
+Iniculating Needle بعمل طعن للمنطقة اللي تحت بعدين
+
+383
+00:29:18,750 --> 00:29:22,630
+بطلع بعمل ريكزاج للمنطقة المائية هذا بيكون السطح
+
+384
+00:29:22,630 --> 00:29:27,550
+بعمل ريكزاج عشان أزرع على هذا السطح البكتيريا طب
+
+385
+00:29:27,550 --> 00:29:32,030
+ليش بزرع البكتيريا بطريقة الـ Flat Etude؟ in order
+
+386
+00:29:32,030 --> 00:29:36,510
+to get a flat iniculating surface it used for
+
+387
+00:29:36,510 --> 00:29:40,840
+transport and storageهذه الحالة بيكون عندى مساحة
+
+388
+00:29:40,840 --> 00:29:44,900
+سطح أكبر يبقى بحصل على كمية أكبر من البكتيريا
+
+389
+00:29:44,900 --> 00:29:49,660
+بيكون مناسب في حالة ال transport في حالة النقل أو
+
+390
+00:29:49,660 --> 00:29:52,860
+لل storage التخزين لو بدي أخزن البكتيريا ال
+
+391
+00:29:52,860 --> 00:29:57,340
+slanted tube مناسبة لأنها بتوفر مساحة سطح أكبر
+
+392
+00:29:57,340 --> 00:30:01,760
+تالت شكل من أشكال ال cultural media للـ tab Q4DP
+
+393
+00:30:01,760 --> 00:30:05,840
+نفس ال slanted tube أنابيب تحتوي على وسط غذائي
+
+394
+00:30:05,840 --> 00:30:10,520
+معهد للـ Designing أجارلكن الفرق الإختلاف عن الـ
+
+395
+00:30:10,520 --> 00:30:14,160
+slanted tube إنها مش بزاوية بتكون عادي بطلّحها من
+
+396
+00:30:14,160 --> 00:30:18,180
+الـ autoclave بحطها في الرّاك تتصلب، بتركها تتصلب
+
+397
+00:30:18,180 --> 00:30:23,200
+لأن طريقة الزراعة انيكولاتية بيستعمل تطعن
+
+398
+00:30:23,200 --> 00:30:27,580
+الانيكولام إلى أجار الانيكولام هي المسحة اللي أنا
+
+399
+00:30:27,580 --> 00:30:31,560
+أخدتها أو البكتيريا بطعنها في الأجار بفحص الـ
+
+400
+00:30:31,560 --> 00:30:37,290
+motility أجار إبلات خلّصنا من ال tubeالـ tube إما
+
+401
+00:30:37,290 --> 00:30:41,150
+يكون بروس ممكن يكون slanted tube ممكن يكون stab
+
+402
+00:30:41,150 --> 00:30:45,250
+tube لكن البلاد فقط أجار لأنه مابقدر أحط بروس في
+
+403
+00:30:45,250 --> 00:30:50,350
+البلاد are sterile petri-blets قولنا إنه الـ petri
+
+404
+00:30:50,350 --> 00:30:54,750
+-blets بيجيب استيرايل معقّم في bags that are
+
+405
+00:30:54,750 --> 00:30:59,070
+aseptically filled with a melted sterile agar
+
+406
+00:30:59,070 --> 00:31:03,990
+media and allowed to solidify أنا بصب الميديا في
+
+407
+00:31:03,990 --> 00:31:09,930
+الأطباقبشكل طبعا بشكل اني انا امنع يكون تماناش
+
+408
+00:31:09,930 --> 00:31:13,950
+Aseptically بعمل كل الإجراءات اللازمة لمنع يكون
+
+409
+00:31:13,950 --> 00:31:17,830
+تماناش بصب ال media في الأطباق هاي ال media بتكون
+
+410
+00:31:17,830 --> 00:31:23,070
+استيرال معقمة وبعدين بتركها تتصلق بلايز are much
+
+411
+00:31:23,070 --> 00:31:27,610
+less confining than slant and stack بقولي البلايز
+
+412
+00:31:27,610 --> 00:31:31,210
+بتكون أقل قيود من ال slant و ال stack عشان هي أنا
+
+413
+00:31:31,210 --> 00:31:35,670
+بستخدمها in the culture inالزراعة عشان أحصل على
+
+414
+00:31:35,670 --> 00:31:39,750
+isolated colony و أقدر أعمل pure culture و أتعرف
+
+415
+00:31:39,750 --> 00:31:46,650
+على البكتيريا سيبراتينج قلنا أنه أنا من خلال طريقة
+
+416
+00:31:46,650 --> 00:31:50,450
+الأرباع بقدر أفصل المستعمرات على البعض و أحصل على
+
+417
+00:31:50,450 --> 00:31:55,090
+isolated colony and counting of microorganisms لو
+
+418
+00:31:55,090 --> 00:31:59,730
+زرعت على إسلام مش هقدر أني أعد ال micro organism
+
+419
+00:31:59,730 --> 00:32:04,550
+لكن بالطبقيا لمساحة وارتيا بقدر أعدفي كمان شغلة
+
+420
+00:32:04,550 --> 00:32:09,650
+تالتة بقدر من خلالها أو بتفيدني فيه البلات اللي هي
+
+421
+00:32:09,650 --> 00:32:14,190
+فحص المضادات الحيوية طبعا الفحص المضادات الحيوية
+
+422
+00:32:14,190 --> 00:32:18,350
+الطبق أفضل ما بقدر أعمل هذا الفحص let you pick up
+
+423
+00:32:18,350 --> 00:32:22,530
+البلات is less confining than slants and steps
+
+424
+00:32:22,530 --> 00:32:26,170
+تستخدم في ال capturing, separating and counting
+
+425
+00:32:26,170 --> 00:32:31,060
+وفي كمان في فحص المضادات الحيويةطرق زراعة لبلاد
+
+426
+00:32:31,060 --> 00:32:40,760
+هيتعرف عليها في آخر المعمل هان
+
+427
+00:32:40,760 --> 00:32:44,120
+أشكال الـ Cultural Media قولنا لتيوب في عندي تلات
+
+428
+00:32:44,120 --> 00:32:47,800
+أشكال ممكن تكون البروست ميديا لكن ما بتحتوي على
+
+429
+00:32:47,800 --> 00:32:53,220
+أجار ممكن تكون أجار Deep اللي هي فيها أصل الـ
+
+430
+00:32:53,220 --> 00:32:57,500
+Dividing Agent أجارلكن بتكون بشكل عادي بتركها على
+
+431
+00:32:57,500 --> 00:33:02,100
+ذاك تتطلب فتاخد الشكل العادي بالنسبة للأجار slant
+
+432
+00:33:02,100 --> 00:33:05,220
+بتفرق عن الأجار دي فيها طبعا في ال device agent
+
+433
+00:33:05,220 --> 00:33:10,900
+لكن بتكون بزاوية بتكون مائلة بالنسبة لبلاد أكيد
+
+434
+00:33:10,900 --> 00:33:17,140
+هتكون أجار إبلات هنأشكال
+
+435
+00:33:17,140 --> 00:33:20,340
+ال culture media اللي فيها أجار اللي هي لبلاد
+
+436
+00:33:20,340 --> 00:33:27,070
+الأجار دي والأجار slantفيها مقارنة لمساحة السطح
+
+437
+00:33:27,070 --> 00:33:30,410
+أكبر مساحة السطح أكيد هتكون الأجاري بلايك بعدين
+
+438
+00:33:30,410 --> 00:33:34,190
+الأجاري slant لكن الأجاري tube مافي عندي أي مساحة
+
+439
+00:33:34,190 --> 00:33:40,970
+سطح كيف بدي أحصل على ال slant tube ممكن أني أنا
+
+440
+00:33:40,970 --> 00:33:44,470
+أحط ال tube بعد ما أطلعها من ال autoclave على أي
+
+441
+00:33:44,470 --> 00:33:49,610
+سطح بشكل مائل فبعد فترة هتتطلب وتعطيني ال slant
+
+442
+00:33:49,610 --> 00:33:53,610
+tubeممكن فيه ان دي جهاز بينحط عليه إجراءات بشكل
+
+443
+00:33:53,610 --> 00:33:57,430
+مائل فبالتالي بيتكون لـ slant tube زي ما احنا
+
+444
+00:33:57,430 --> 00:34:01,710
+ملاحظين في الصورة
+
+445
+00:34:01,710 --> 00:34:05,550
+Aseptic technique يعني كل الإجراءات اللتي يتم
+
+446
+00:34:05,550 --> 00:34:09,070
+اتخاذها لمنع الـ contamination the most commonly
+
+447
+00:34:09,070 --> 00:34:12,590
+used device for moving bacteria is the inculating
+
+448
+00:34:12,590 --> 00:34:16,830
+loop بقول لي إن الـinculating loop هي أدات استخدام
+
+449
+00:34:16,830 --> 00:34:21,210
+لنقل بكتيريا من وسط لآخر وزراعتهاالـ Nucleating
+
+450
+00:34:21,210 --> 00:34:24,610
+loop ممكن أنها تكون من النيكروم النيكروم and alloy
+
+451
+00:34:24,610 --> 00:34:28,730
+هي سبيكة of nickel and chromium أو ممكن تكون من
+
+452
+00:34:28,730 --> 00:34:33,170
+البلاتينيوم wire بالنسبة للـ Nucleating loop
+
+453
+00:34:33,170 --> 00:34:37,230
+بنلاحظ أن في عندي أنا حلقة loop at one end في
+
+454
+00:34:37,230 --> 00:34:40,610
+نهايتها في حلقها الحلقة هي اللي باخد البكتيريا
+
+455
+00:34:40,610 --> 00:34:44,890
+فيها والجزء الثاني اللي هو أنا بمسك وهذا بالنسبة
+
+456
+00:34:44,890 --> 00:34:48,790
+للـ Nucleating loopفي أداء تشبهها اسمها الـ
+
+457
+00:34:48,790 --> 00:34:52,050
+Inculating Needle اللي احنا استخدمناها في الـ
+
+458
+00:34:52,050 --> 00:34:55,850
+Motility هي نفس الشيء لكن مش موجود في Handy Loop
+
+459
+00:34:55,850 --> 00:35:04,110
+مافي Handy حلقة هي Stressed Wire كيف
+
+460
+00:35:04,110 --> 00:35:07,350
+انا بدي اعقم الـ Inculating Loop أو الـ Needle؟
+
+461
+00:35:07,350 --> 00:35:10,750
+استيريليز بواب إنسترمنت بحافظ على جزء الـ Wire في
+
+462
+00:35:10,750 --> 00:35:15,110
+الأسلام حتى يشعروا بالإنسناراش بعقم الأدتان
+
+463
+00:35:15,110 --> 00:35:20,820
+بتعريضهم لللهبلما تتوهج glow يصير لونه أحمر هيك
+
+464
+00:35:20,820 --> 00:35:24,680
+بيكون تم إبتعاقيم the instrument should be allowed
+
+465
+00:35:24,680 --> 00:35:29,060
+to cool قولي بعد ما تم عملية التعقيم بستنى غلق
+
+466
+00:35:29,060 --> 00:35:33,240
+اللوب أو ال needle إنها تبرد لمدة عشر لعشرين
+
+467
+00:35:33,240 --> 00:35:37,660
+second قبل ما أنا أستخدمها why to avoid killing
+
+468
+00:35:37,660 --> 00:35:41,920
+the iniculum عشان ما أقتل ال iniculum ال iniculum
+
+469
+00:35:41,920 --> 00:35:45,290
+المسح اللي أنا أخدتهاin this way all contaminants
+
+470
+00:35:45,290 --> 00:35:49,250
+on the wire are incinerated في هذه الطريقة كل ال
+
+471
+00:35:49,250 --> 00:35:52,990
+contaminants اللي على ال wire أو على ال loop
+
+472
+00:35:52,990 --> 00:35:57,470
+بيصلوا incinerated فيها ملاحظات don't blow on the
+
+473
+00:35:57,470 --> 00:36:00,950
+instrument to cool them ممنوع أني أنفخ على ال loop
+
+474
+00:36:00,950 --> 00:36:05,350
+أو ال needle عشان أخليه يبرد ممنوع don't touch the
+
+475
+00:36:05,350 --> 00:36:09,090
+instrument to a car to cool them ممنوع أعمل touch
+
+476
+00:36:09,090 --> 00:36:12,850
+بالأجار عشان أخليه يبردDon't lay the loop down
+
+477
+00:36:12,850 --> 00:36:17,170
+once it is sterilized ممنوع أخلي اللوب أو الـ
+
+478
+00:36:17,170 --> 00:36:20,470
+needle بعد ما أعقمه أحطه على البرج لأنه بيصير
+
+479
+00:36:20,470 --> 00:36:27,010
+contaminant procedure
+
+480
+00:36:27,010 --> 00:36:31,050
+for aseptically transfer أول شي بدي أعقم اللوب
+
+481
+00:36:31,420 --> 00:36:34,220
+واللي تاني إشي بدون ما تحط اللوب على الـ bench
+
+482
+00:36:34,220 --> 00:36:38,160
+بتنسكي الـ culture tube بتعقم الفوهة بتمريرها على
+
+483
+00:36:38,160 --> 00:36:42,260
+اللهد بسرعة ممنوع أحط الغطاية على الـ bench بضلي
+
+484
+00:36:42,260 --> 00:36:46,500
+مسكاها بإيدي الغطاية بنسكها بنفس الإيدي اللي ماسكا
+
+485
+00:36:46,500 --> 00:36:52,460
+فيها اللوب تالت إشي بدخل اللوب في الـ tube و بذرع
+
+486
+00:36:52,460 --> 00:36:56,700
+رابع إشي بعقم بعد ما أخلص الشغل بعقم الفوهة و
+
+487
+00:36:56,700 --> 00:37:01,620
+بفكرهاخامس شاق بكرر نفس الخطوات في حال أنا ذراعت
+
+488
+00:37:01,620 --> 00:37:08,780
+أكتر من الـ tube لما
+
+489
+00:37:08,780 --> 00:37:13,340
+أخلص الشغل بعقم اللوب و بحطه على ال bench لو أنا
+
+490
+00:37:13,340 --> 00:37:19,720
+فكت أني أنا ما عقمت، بعقم هان
+
+491
+00:37:19,720 --> 00:37:24,400
+الخطوات في صور بعقم اللوب بتعريضه لأ اللهب لما
+
+492
+00:37:24,400 --> 00:37:28,850
+يصير لونه أحمر يتوهجك وعرف تم تعقيمهفخلّيه يبرد
+
+493
+00:37:28,850 --> 00:37:34,870
+قبل ما أستخدمه من 10 ل 20 second عشان أمنع ال
+
+494
+00:37:34,870 --> 00:37:40,830
+killing للانيكولام بمسك
+
+495
+00:37:40,830 --> 00:37:45,630
+ال capture cube برفع الغطاية بعقم الفوهة ممنوع أحط
+
+496
+00:37:45,630 --> 00:37:50,410
+الغطاية على ال bench بزرع بعد ما أخلص الشغل بعقم
+
+497
+00:37:50,410 --> 00:37:52,830
+الفوهة و برجع الغطاية
+
+498
+00:37:58,120 --> 00:38:02,160
+الزائد اللي هبط أول جزء بيكون dark zone of
+
+499
+00:38:02,160 --> 00:38:06,460
+unburned gas هذه المنطقة ما بيصير فيها احتراف
+
+500
+00:38:06,460 --> 00:38:10,060
+بعدين عندي ال orange zone of incomplete combustion
+
+501
+00:38:10,060 --> 00:38:13,600
+بعدين بتيجي منطقة ال blue zone of partial
+
+502
+00:38:13,600 --> 00:38:17,400
+combustion أخر شئ ال colorless zone of complete
+
+503
+00:38:17,400 --> 00:38:21,860
+combustion كل ما طلعنا لفوق بيزيد ال oxygen بيصير
+
+504
+00:38:21,860 --> 00:38:26,400
+sufficient combustion احتراف كامل للغازفبالتالي
+
+505
+00:38:26,400 --> 00:38:30,260
+بتكون شديدة الحرارة hottest يبقى اللي بدنا نعرفه
+
+506
+00:38:30,260 --> 00:38:34,820
+أن التعقيد بيحصل عند المنطقة الزرقه أو المنطقة ال
+
+507
+00:38:34,820 --> 00:38:40,520
+colorless بتميها non-luminous flame بتكون hottest
+
+508
+00:38:40,520 --> 00:38:45,400
+بتكون شديدة الحرارة لكن لل luminous flame بتكون هي
+
+509
+00:38:45,400 --> 00:38:50,660
+المنطقة ال orange not very hotماذا تستخدمها في
+
+510
+00:38:50,660 --> 00:38:55,180
+تعقيم اللوب بستخدم فيما تعقيم اللوب تعقيم عند
+
+511
+00:38:55,180 --> 00:39:00,100
+المنطقة ال blue، الزرق أو ال colorless لأنه بتكون
+
+512
+00:39:00,100 --> 00:39:04,920
+hottest five
+
+513
+00:39:04,920 --> 00:39:10,100
+basic techniques of culturing إنكولات فتحت لتيوب
+
+514
+00:39:10,100 --> 00:39:14,000
+أو لبلات و أخدت إنكولام و زرعت بعد ما زرعت دخلت
+
+515
+00:39:14,000 --> 00:39:17,040
+الطبق على ال incubator و عملت incubation يبقى
+
+516
+00:39:17,040 --> 00:39:21,620
+المرحلة التانية incubationمدت 24 ساعة و أحيانا 48
+
+517
+00:39:21,620 --> 00:39:25,640
+حسب البكتيريا بعد الـ incubation هلاقي نمو
+
+518
+00:39:25,640 --> 00:39:29,660
+للبكتيريا ممكن ينمو أكتر من نوع من البكتيريا باخد
+
+519
+00:39:29,660 --> 00:39:32,920
+كل نوع و بزرعه على طبق هاي المرحلة بسميها
+
+520
+00:39:32,920 --> 00:39:37,360
+isolation يبقى المرحلة التانية ال isolation بعدها
+
+521
+00:39:37,360 --> 00:39:41,480
+هعمل ححوقات تعريفية في مرحلة ال examination عشان
+
+522
+00:39:41,480 --> 00:39:45,240
+أعمل identification للبكتيريا اللي هي المرحلة
+
+523
+00:39:45,240 --> 00:39:45,940
+الأخيرة
+
+524
+00:39:49,790 --> 00:39:53,370
+Pure culture concept to identify bacteria in a
+
+525
+00:39:53,370 --> 00:39:56,670
+clinical sample cannot be done unless isolated
+
+526
+00:39:56,670 --> 00:40:00,290
+colony are used عشان أتعرف على البكتيريا لازم يكون
+
+527
+00:40:00,290 --> 00:40:04,490
+عندي isolated colony ال isolated colony أصلها خلية
+
+528
+00:40:04,490 --> 00:40:09,270
+بكتيرية واحدة كيف بدي أحصل على ال isolated colony؟
+
+529
+00:40:09,270 --> 00:40:13,190
+to obtain well isolated colony it is essential to
+
+530
+00:40:13,190 --> 00:40:17,270
+disperse the inoculum or sample on the surface of
+
+531
+00:40:17,270 --> 00:40:21,570
+an enriched agar plateعشان أحصل على إيزولات
+
+532
+00:40:21,570 --> 00:40:26,110
+الكلوني مهم جدا أوزع العينة على سطح البلاد أجابي
+
+533
+00:40:26,110 --> 00:40:29,830
+So the individual bacteria are well separated from
+
+534
+00:40:29,830 --> 00:40:34,230
+each other هنقدر نفصل البكتيريا عن بعض بطريقة
+
+535
+00:40:34,230 --> 00:40:39,810
+الأربع، هنخطف فبالتالي هنفصل المستعمرات عن بعض، هك
+
+536
+00:40:39,810 --> 00:40:44,790
+بضمن أن الإيزولات الكلوني أصلها خلية بكتيرية واحدة
+
+537
+00:40:45,190 --> 00:40:48,610
+لكن لو أنا أخدت من الرُبع الأول الرُبع الأول بيكون
+
+538
+00:40:48,610 --> 00:40:52,850
+فك المستعمرات فوق بعض ممكن تكون بكتيريا X فوق
+
+539
+00:40:52,850 --> 00:40:56,790
+بكتيريا Y يعني بيكون عندي mixed population روحت
+
+540
+00:40:56,790 --> 00:41:00,910
+أنا أشتغلت حوصات هيكون كل نتائج الحوصات غلط لأنه
+
+541
+00:41:00,910 --> 00:41:04,630
+أشتغلت على بكتيريا X و بكتيريا Y لازم فقط أنا
+
+542
+00:41:04,630 --> 00:41:08,650
+أشتغل على بكتيريا واحدة في ال isolated colony
+
+543
+00:41:08,650 --> 00:41:14,740
+بتكونالمتعمرات بعيدة عن بعض فضمن أن أصلها بكتيريا
+
+544
+00:41:14,740 --> 00:41:19,100
+واحدة
+
+545
+00:41:19,100 --> 00:41:25,660
+Contaminants
+
+546
+00:41:25,660 --> 00:41:29,480
+other microorganisms present in the sample كائنات
+
+547
+00:41:29,480 --> 00:41:33,500
+أخرى موجودة في العين أو في المزرعة فلسبيل المثال
+
+548
+00:41:33,500 --> 00:41:37,990
+إذا طينت يورين وزرعتها لكن ما عقمت اللوبهينمو
+
+549
+00:41:37,990 --> 00:41:41,830
+micro organism على الوساط غير الموجود في عينة الـ
+
+550
+00:41:41,830 --> 00:41:46,390
+urine نتيجة عدم تعقيمي للـ loop isolated colonies
+
+551
+00:41:46,390 --> 00:41:49,390
+a population of millions of cells that are
+
+552
+00:41:49,390 --> 00:41:53,970
+identical and are descended from a single founder
+
+553
+00:41:53,970 --> 00:41:57,690
+cell ال isolated كولوني لو أنا زرعت في طريقة
+
+554
+00:41:57,690 --> 00:42:01,130
+الأربعة هتكون موجودة في القبع الأخير هي ملايين
+
+555
+00:42:01,130 --> 00:42:05,970
+الخلايا المتشابهة اللي أصلها خلية بكتيرية واحدةلو
+
+556
+00:42:05,970 --> 00:42:09,150
+أخدتها وروح اتزرعتها على طبق بسميه طبق pure
+
+557
+00:42:09,150 --> 00:42:13,490
+culture هيك بيصير كل الطبق من بكتيريا واحدة بلاحظ
+
+558
+00:42:13,490 --> 00:42:18,690
+ان كل الكولوني نفس الموارفولوجي Stock culture،
+
+559
+00:42:18,690 --> 00:42:21,570
+stock يعني مخزون، a culture that already contains
+
+560
+00:42:21,570 --> 00:42:26,570
+cells it is used as source of cell from which to
+
+561
+00:42:26,570 --> 00:42:31,450
+manipulate new cultureتحتوي على خلايا تستخدم كمصدر
+
+562
+00:42:31,450 --> 00:42:35,310
+للخلايا البكتيرية عشان تزرع مرة تانية الأطباق اللي
+
+563
+00:42:35,310 --> 00:42:38,990
+بتزرع منها و بتاخدوا بكتيريا تعتبر stock culture
+
+564
+00:42:38,990 --> 00:42:43,930
+عشان تعملوا a new culture ال culture media ممكن
+
+565
+00:42:43,930 --> 00:42:48,750
+تكون rich غنية بمواد غذائية بتنمي كل الأنواع or
+
+566
+00:42:48,750 --> 00:42:52,590
+selective بتنمي نوع معين ال growth فيها مباديات
+
+567
+00:42:52,590 --> 00:42:57,100
+لنمو البكتيريا or inhibitor فيها مصبطاتممكن تكون
+
+568
+00:42:57,100 --> 00:43:01,580
+Liquid او Solid الـ Temperature لازم تكون مناسبة
+
+569
+00:43:01,580 --> 00:43:06,000
+لنمو البكتيريا Source of Energy مفضلة الطاقة زي
+
+570
+00:43:06,000 --> 00:43:10,280
+الجليكوز، اللاكتوز، المانيتال Source of Carbon او
+
+571
+00:43:10,280 --> 00:43:14,240
+نيتروجين أكيد لازم يكون في عندي Carbon Source او
+
+572
+00:43:14,240 --> 00:43:18,060
+Nitrogen Source عشان تنمو البكتيريا وتتكثر من
+
+573
+00:43:18,060 --> 00:43:22,700
+أمثلة على Carbon Source الكربوهيدرات الـNitrogen
+
+574
+00:43:22,700 --> 00:43:26,810
+Source الأمينو أساسيAseptic techniques include
+
+575
+00:43:26,810 --> 00:43:30,090
+sterilization of media لازم اني انا اعقم ال media
+
+576
+00:43:30,090 --> 00:43:34,870
+sterilization of equipment لازم انا اعقم الأدوات
+
+577
+00:43:34,870 --> 00:43:40,050
+proper handling اني انا اشتغل مثلا جنب اللهد ممنوع
+
+578
+00:43:40,050 --> 00:43:43,350
+اني انا انفخ عشان يبرد اللوب اتعقم ال bench قبل
+
+579
+00:43:43,350 --> 00:43:47,410
+الشغل وبعد هذا كلها تعتبر aseptic technique عشان
+
+580
+00:43:47,410 --> 00:43:48,710
+امنع ال contamination
+
+581
+00:43:55,320 --> 00:43:59,720
+الأدوات اللي هستخدمها في الشغل Maniculating Loops
+
+582
+00:43:59,720 --> 00:44:04,580
+لها بنسن للتعقيم stop culture قلنا ان stop culture
+
+583
+00:44:04,580 --> 00:44:08,320
+هي مخزون تحتوي على خلايا عشان انا اعمل a new
+
+584
+00:44:08,320 --> 00:44:12,540
+culture اكيد استرال اجار ابلات اللي انا بدي ازرع
+
+585
+00:44:12,540 --> 00:44:12,840
+عليه
+
+586
+00:44:20,110 --> 00:44:24,510
+كيف طريقة الشغل عشان أحصل على isolated colony أول
+
+587
+00:44:24,510 --> 00:44:29,770
+خطوة بعقّم اللوب باتسنع يبرد باخد انيكلوم من الـ
+
+588
+00:44:29,770 --> 00:44:35,050
+Soap culture وأكيد بعقّم الفوا بفرض الانيكلوم على
+
+589
+00:44:35,050 --> 00:44:38,990
+الرُبع الأول بحاول يكون continuous تقريب عن بعض
+
+590
+00:44:38,990 --> 00:44:42,370
+تاني خطوة بتوقع أنا في الرُبع الأول مش هيكون
+
+591
+00:44:42,370 --> 00:44:46,270
+isolated colony بحاول اني انا ما اضغط على الأجار
+
+592
+00:44:46,270 --> 00:44:50,850
+وانا بصرا أو استخدمت plastic loop بتخلص منهثالث
+
+593
+00:44:50,850 --> 00:44:54,910
+خطوة بقولي تقلبي الطبق 90 درجة و تزرعي الربع
+
+594
+00:44:54,910 --> 00:44:59,070
+التاني تاخدي من الربع الأول و تعملي أخطوط ما ترجعي
+
+595
+00:44:59,070 --> 00:45:07,430
+تاخدي من ال stock لأن أنا بدي أحفظ بكرر
+
+596
+00:45:07,430 --> 00:45:12,450
+نفس الخطوات في الربع التالت و في الربع الرابع
+
+597
+00:45:12,450 --> 00:45:16,570
+بحاول أني أتخلص من ال loop بين ربع و ربع لو أنا
+
+598
+00:45:16,570 --> 00:45:20,820
+استخدمت plastic loop اذا نيكروم loop بعقمالنيكروم
+
+599
+00:45:20,820 --> 00:45:24,900
+لوب آخر إشي بقولي أنا بحط الأطباق في ال incubator
+
+600
+00:45:24,900 --> 00:45:29,240
+upside-down why؟ to prevent condensation حتى أمنع
+
+601
+00:45:29,240 --> 00:45:34,200
+التكاثر فيها الملاحظة أنه أنا لما أفتكر tube بحاول
+
+602
+00:45:34,200 --> 00:45:37,580
+أني أنا ما أمسكها من ال LED لأن ممكن أنها تكون
+
+603
+00:45:37,580 --> 00:45:45,500
+مفتوحة وتنكسر منها هذه
+
+604
+00:45:45,500 --> 00:45:50,590
+الخطوات في سؤال أول إشي بعقم ال loopباخد Eniculum
+
+605
+00:45:50,590 --> 00:45:54,250
+وبذرع الربع الأول بشكل continuous بكونه اقرب عن
+
+606
+00:45:54,250 --> 00:46:00,410
+بعض بعدين بعقم اللوب بقلب البلاد 90 درجة باخد من
+
+607
+00:46:00,410 --> 00:46:05,670
+الربع الأول و بطلع بعدها بقلب 90 درجة و طبعا بعقم
+
+608
+00:46:05,670 --> 00:46:11,290
+اللوب باخد من الربع التاني و بطلع بقلب 90 درجة و
+
+609
+00:46:11,290 --> 00:46:15,810
+أكيد بعقم اللوب و باخد من الربع التالت و بعمل deep
+
+610
+00:46:15,810 --> 00:46:16,210
+bag
+
+611
+00:46:20,320 --> 00:46:24,160
+في عندي تلت طرق لزراعة الأجار إبلاد أول طريقة
+
+612
+00:46:24,160 --> 00:46:26,400
+تعرفنا عليها بالـ slide الأقبل اللي هي الـ
+
+613
+00:46:26,400 --> 00:46:30,480
+Quadrant String هاي الطريقة بستخدمها لحصول على
+
+614
+00:46:30,480 --> 00:46:34,360
+إيزولات كولوني لعمل بيورت كالتشار عشان أتعرف على
+
+615
+00:46:34,360 --> 00:46:38,760
+البكتيريا الطريقة التانية الـ Radiant String اللي
+
+616
+00:46:38,760 --> 00:46:43,860
+هي الشعاعي بستخدمها حتى أحصل أيضا على إيزولات
+
+617
+00:46:43,860 --> 00:46:47,680
+كولوني كفي طريقة الزراعة بزرعالربع الأول بشكل
+
+618
+00:46:47,680 --> 00:46:53,820
+continuous بعدين بطلع شعاع من الربع الأول بين كل
+
+619
+00:46:53,820 --> 00:46:58,660
+شعاع و التاني بعقن بعدين بقلب 90 درجة و بحاول أني
+
+620
+00:46:58,660 --> 00:47:03,320
+انا أطلع شعاع و بعقن بين كل شعاع و التاني هذه
+
+621
+00:47:03,320 --> 00:47:07,240
+الـVariant Structure الطريقة التالتة ال continuous
+
+622
+00:47:07,240 --> 00:47:12,200
+أو الـ Z-zag هذه الطريقة بنستخدمها لفحص المضادات
+
+623
+00:47:12,200 --> 00:47:18,120
+الحيوية و كمان بنستخدمها ل ال countطبعا بفتح أنا
+
+624
+00:47:18,120 --> 00:47:22,720
+الطبق بزرع بشكل continuous لازم يكون نتح كل الطبق
+
+625
+00:47:22,720 --> 00:47:32,300
+بالبكتيريا هيك تسمو كل البكتيريا على الطبق هاي
+
+626
+00:47:32,300 --> 00:47:36,500
+الطريقة بستخدمها في ال urine culture فنزل خط بعدين
+
+627
+00:47:36,500 --> 00:47:41,720
+بزرع بشكل continuous بالنسبة للثورة high الثورة
+
+628
+00:47:41,720 --> 00:47:46,910
+high الطبق مزروع بطريقة radiant spreadلكن هذه
+
+629
+00:47:46,910 --> 00:47:51,930
+الصورة مزروعة بطريقة الـ Quadrant Effect بحصل على
+
+630
+00:47:51,930 --> 00:47:57,050
+إيزولاتت كولونية على مستعمرات مفصولة عن بعض بقدر
+
+631
+00:47:57,050 --> 00:48:01,490
+أعمل منها view of culture و أتعرف على البكتيريا
+
+632
+00:48:01,490 --> 00:48:06,610
+خلصنا الشطر ياتيكم العافية جميعا والسلام عليكم
+
+633
+00:48:06,610 --> 00:48:08,250
+ورحمة الله وبركاته
+
diff --git a/PL9fwy3NUQKwZWgAb_V1ATlRRsH-6i3cPC/ek7K6HogOhs_raw.json b/PL9fwy3NUQKwZWgAb_V1ATlRRsH-6i3cPC/ek7K6HogOhs_raw.json
new file mode 100644
index 0000000000000000000000000000000000000000..133db3f09460f130a396739122fb5cb574fcd0fc
--- /dev/null
+++ b/PL9fwy3NUQKwZWgAb_V1ATlRRsH-6i3cPC/ek7K6HogOhs_raw.json
@@ -0,0 +1 @@
+{"segments": [{"id": 1, "seek": 3107, "start": 21.23, "end": 31.07, "text": "السلام عليكم ورحمة الله وبركاته، نستكمل معكم سلسلة Microbiology الجامعة الإسلامية، فيديو اليوم بعنوان «جرام الثاني»", "tokens": [6027, 3794, 37440, 25894, 24793, 4032, 2288, 35571, 3660, 21984, 4032, 26890, 4117, 9307, 3224, 12399, 8717, 14851, 24793, 1211, 20449, 24793, 8608, 1211, 3794, 37977, 5818, 20027, 1793, 25724, 10943, 27884, 33688, 3794, 37440, 10632, 12399, 8978, 16254, 2407, 45595, 20498, 45030, 1863, 2407, 7649, 4657, 7435, 2288, 10943, 6024, 104, 7649, 1829, 5933], "avg_logprob": -0.18582589126058988, "compression_ratio": 1.3716216216216217, "no_speech_prob": 1.1920928955078125e-07, "words": [{"start": 21.23, "end": 21.69, "word": "السلام", "probability": 0.9235026041666666}, {"start": 21.69, "end": 22.23, "word": " عليكم", "probability": 0.9541015625}, {"start": 22.23, "end": 22.73, "word": " ورحمة", "probability": 0.9344482421875}, {"start": 22.73, "end": 22.95, "word": " الله", "probability": 0.974609375}, {"start": 22.95, "end": 24.01, "word": " وبركاته،", "probability": 0.8774007161458334}, {"start": 24.01, "end": 24.53, "word": " نستكمل", "probability": 0.977783203125}, {"start": 24.53, "end": 25.07, "word": " معكم", "probability": 0.831298828125}, {"start": 25.07, "end": 25.73, "word": " سلسلة", "probability": 0.990234375}, {"start": 25.73, "end": 26.61, "word": " Microbiology", "probability": 0.6199544270833334}, {"start": 26.61, "end": 27.19, "word": " الجامعة", "probability": 0.95703125}, {"start": 27.19, "end": 28.41, "word": " الإسلامية،", "probability": 0.87783203125}, {"start": 28.41, "end": 28.73, "word": " فيديو", "probability": 0.9527994791666666}, {"start": 28.73, "end": 29.11, "word": " اليوم", "probability": 0.9765625}, {"start": 29.11, "end": 29.91, "word": " بعنوان", "probability": 0.918701171875}, {"start": 29.91, "end": 30.47, "word": " «جرام", "probability": 0.582183837890625}, {"start": 30.47, "end": 31.07, "word": " الثاني»", "probability": 0.809423828125}], "temperature": 1.0}, {"id": 2, "seek": 7887, "start": 56.65, "end": 78.87, "text": "والآن لنتعرف عن الأدوات التي تستخدم في خطوات العمل أولاً لدينا الصبغة الأولية وهي gram crystal violet ثم لدينا gram iodine وهو المثبت ثم لدينا gram decalorizer وهو عبارة عن ميثانو بتركيز 95%", "tokens": [2407, 6027, 148, 48506, 5296, 29399, 3615, 28480, 18871, 16247, 3215, 2407, 9307, 38392, 6055, 14851, 9778, 40448, 8978, 16490, 9566, 2407, 9307, 18863, 42213, 5551, 12610, 995, 14111, 5296, 16254, 8315, 31767, 3555, 17082, 3660, 16247, 12610, 10632, 37037, 1829, 21353, 13662, 46480, 38637, 2304, 5296, 16254, 8315, 21353, 44422, 533, 37037, 2407, 9673, 12984, 3555, 2655, 38637, 2304, 5296, 16254, 8315, 21353, 979, 304, 284, 6545, 37037, 2407, 6225, 3555, 9640, 3660, 18871, 3714, 1829, 12984, 7649, 2407, 4724, 2655, 31747, 1829, 11622, 13420, 4], "avg_logprob": -0.18465909666635774, "compression_ratio": 1.5303030303030303, "no_speech_prob": 2.2649765014648438e-06, "words": [{"start": 56.65, "end": 57.03, "word": "والآن", "probability": 0.61865234375}, {"start": 57.03, "end": 57.55, "word": " لنتعرف", "probability": 0.8424072265625}, {"start": 57.55, "end": 57.71, "word": " عن", "probability": 0.62060546875}, {"start": 57.71, "end": 58.15, "word": " الأدوات", "probability": 0.9879150390625}, {"start": 58.15, "end": 58.41, "word": " التي", "probability": 0.83740234375}, {"start": 58.41, "end": 58.91, "word": " تستخدم", "probability": 0.9544677734375}, {"start": 58.91, "end": 59.07, "word": " في", "probability": 0.94287109375}, {"start": 59.07, "end": 59.47, "word": " خطوات", "probability": 0.9542236328125}, {"start": 59.47, "end": 59.87, "word": " العمل", "probability": 0.996337890625}, {"start": 59.87, "end": 60.75, "word": " أولاً", "probability": 0.7779541015625}, {"start": 60.75, "end": 61.29, "word": " لدينا", "probability": 0.89697265625}, {"start": 61.29, "end": 62.19, "word": " الصبغة", "probability": 0.8494873046875}, {"start": 62.19, "end": 62.75, "word": " الأولية", "probability": 0.9708658854166666}, {"start": 62.75, "end": 63.25, "word": " وهي", "probability": 0.85009765625}, {"start": 63.25, "end": 63.77, "word": " gram", "probability": 0.150146484375}, {"start": 63.77, "end": 64.15, "word": " crystal", "probability": 0.488525390625}, {"start": 64.15, "end": 64.61, "word": " violet", "probability": 0.205078125}, {"start": 64.61, "end": 68.01, "word": " ثم", "probability": 0.927978515625}, {"start": 68.01, "end": 68.43, "word": " لدينا", "probability": 0.9786783854166666}, {"start": 68.43, "end": 68.71, "word": " gram", "probability": 0.91455078125}, {"start": 68.71, "end": 69.25, "word": " iodine", "probability": 0.924560546875}, {"start": 69.25, "end": 69.75, "word": " وهو", "probability": 0.845458984375}, {"start": 69.75, "end": 70.43, "word": " المثبت", "probability": 0.9608154296875}, {"start": 70.43, "end": 74.67, "word": " ثم", "probability": 0.96630859375}, {"start": 74.67, "end": 75.27, "word": " لدينا", "probability": 0.9886067708333334}, {"start": 75.27, "end": 75.63, "word": " gram", "probability": 0.94091796875}, {"start": 75.63, "end": 76.33, "word": " decalorizer", "probability": 0.739013671875}, {"start": 76.33, "end": 76.55, "word": " وهو", "probability": 0.906982421875}, {"start": 76.55, "end": 76.89, "word": " عبارة", "probability": 0.9761962890625}, {"start": 76.89, "end": 77.05, "word": " عن", "probability": 0.99267578125}, {"start": 77.05, "end": 77.45, "word": " ميثانو", "probability": 0.86728515625}, {"start": 77.45, "end": 77.93, "word": " بتركيز", "probability": 0.78857421875}, {"start": 77.93, "end": 78.41, "word": " 95", "probability": 0.9658203125}, {"start": 78.41, "end": 78.87, "word": "%", "probability": 0.95263671875}], "temperature": 1.0}, {"id": 3, "seek": 9412, "start": 82.52, "end": 94.12, "text": "وداينا ال counter-stain وهي عبارة عن أصابع المعاكسة الصفرانين, sterile loops, slide, normal saline والبكتيريا، سلبة جرامة وموجة متجرامة", "tokens": [23328, 995, 9957, 995, 2423, 5682, 12, 372, 491, 37037, 1829, 6225, 3555, 9640, 3660, 18871, 5551, 9381, 16758, 3615, 9673, 3615, 995, 4117, 3794, 3660, 31767, 5172, 2288, 7649, 9957, 11, 18924, 794, 16121, 11, 4137, 11, 2710, 1845, 533, 16070, 3555, 4117, 2655, 13546, 25528, 12399, 8608, 46152, 3660, 10874, 2288, 10943, 3660, 4032, 2304, 29245, 3660, 44650, 7435, 2288, 10943, 3660], "avg_logprob": -0.29375, "compression_ratio": 1.29375, "no_speech_prob": 0.0, "words": [{"start": 82.52, "end": 83.08, "word": "وداينا", "probability": 0.656494140625}, {"start": 83.08, "end": 83.2, "word": " ال", "probability": 0.95947265625}, {"start": 83.2, "end": 83.48, "word": " counter", "probability": 0.81201171875}, {"start": 83.48, "end": 83.9, "word": "-stain", "probability": 0.4728597005208333}, {"start": 83.9, "end": 84.16, "word": " وهي", "probability": 0.700927734375}, {"start": 84.16, "end": 84.52, "word": " عبارة", "probability": 0.9847412109375}, {"start": 84.52, "end": 84.72, "word": " عن", "probability": 0.9970703125}, {"start": 84.72, "end": 85.06, "word": " أصابع", "probability": 0.8453369140625}, {"start": 85.06, "end": 85.68, "word": " المعاكسة", "probability": 0.9479166666666666}, {"start": 85.68, "end": 86.42, "word": " الصفرانين,", "probability": 0.91240234375}, {"start": 86.76, "end": 89.36, "word": " sterile", "probability": 0.731201171875}, {"start": 89.36, "end": 89.76, "word": " loops,", "probability": 0.8740234375}, {"start": 90.02, "end": 90.48, "word": " slide,", "probability": 0.521484375}, {"start": 90.84, "end": 91.08, "word": " normal", "probability": 0.8134765625}, {"start": 91.08, "end": 91.58, "word": " saline", "probability": 0.725830078125}, {"start": 91.58, "end": 92.56, "word": " والبكتيريا،", "probability": 0.777099609375}, {"start": 92.56, "end": 92.9, "word": " سلبة", "probability": 0.7057291666666666}, {"start": 92.9, "end": 93.26, "word": " جرامة", "probability": 0.835693359375}, {"start": 93.26, "end": 93.56, "word": " وموجة", "probability": 0.666259765625}, {"start": 93.56, "end": 94.12, "word": " متجرامة", "probability": 0.89765625}], "temperature": 1.0}], "language": "ar", "language_probability": 1.0, "duration": 245.1795, "duration_after_vad": 44.28937499999999}
\ No newline at end of file
diff --git a/PL9fwy3NUQKwZWgAb_V1ATlRRsH-6i3cPC/fZnjal05seA_postprocess.srt b/PL9fwy3NUQKwZWgAb_V1ATlRRsH-6i3cPC/fZnjal05seA_postprocess.srt
new file mode 100644
index 0000000000000000000000000000000000000000..f71d9c95bcd3102dbf298477b0301a87fff6a09a
--- /dev/null
+++ b/PL9fwy3NUQKwZWgAb_V1ATlRRsH-6i3cPC/fZnjal05seA_postprocess.srt
@@ -0,0 +1,848 @@
+1
+00:00:00,830 --> 00:00:03,890
+السلام عليكم اليوم هنحكي عن مساق ل practical
+
+2
+00:00:03,890 --> 00:00:08,230
+essential medical microbiology في هذا اللقاء
+
+3
+00:00:08,230 --> 00:00:13,170
+هنتعرف على علم ال microbiology تعريف هذا العلم
+
+4
+00:00:13,170 --> 00:00:17,790
+فروع علم ال microbiology و الهدف من قسم ال
+
+5
+00:00:17,790 --> 00:00:22,290
+microbiology ال biology في المستشفيات هنتعرف على
+
+6
+00:00:22,290 --> 00:00:27,090
+المواضيع اللي هنتناولها في هذا المساق و توزيها
+
+7
+00:00:27,090 --> 00:00:34,660
+خلال الفصلو آخر شئ هنحكي عن توزيع درجات المساق
+
+8
+00:00:34,660 --> 00:00:41,780
+بالنسبة لعلم ال microbiology لو جزأنا كلمة ال
+
+9
+00:00:41,780 --> 00:00:47,560
+microbiology طبعا هو العلم it is the study بيو
+
+10
+00:00:47,560 --> 00:00:54,950
+حيوي و micro دقيق يبقى هو علم الأحياء الدقيقةالـ
+
+11
+00:00:54,950 --> 00:00:58,330
+Microbiology لو أنا إجابة بدي أعرفه it is the
+
+12
+00:00:58,330 --> 00:01:03,510
+study of microorganism هو علم دراسة الكائنات
+
+13
+00:01:03,510 --> 00:01:08,350
+الدقيقة طب لو بدي أعرف ال micro organism بحكي it
+
+14
+00:01:08,350 --> 00:01:12,650
+is a small living organism that cannot be seen by
+
+15
+00:01:12,650 --> 00:01:17,450
+naked eye هو العلم الذي يدرس الكائنات الدقيقة
+
+16
+00:01:17,450 --> 00:01:23,110
+الحية التي لا ترى بالعين المجردعلم ال microbiology
+
+17
+00:01:23,110 --> 00:01:30,370
+هو علم واسع طبعا له أفرع له أفرع مختلفة ال
+
+18
+00:01:30,370 --> 00:01:36,350
+microbiology يضم عدة فروع medical microbiology و
+
+19
+00:01:36,350 --> 00:01:41,150
+soil microbiology و food microbiology طبعا انتوا
+
+20
+00:01:41,150 --> 00:01:45,890
+في عينكم أساق ال food microbiology اللي طبعا
+
+21
+00:01:45,890 --> 00:01:50,550
+تطبيقات في مجال الأبحاث و في مجال رقابة وجودة
+
+22
+00:01:50,550 --> 00:01:55,140
+الأغذيةفيه إنه كمان Water and Sewage Microbiology
+
+23
+00:01:55,140 --> 00:02:00,360
+Industrial Microbiology وMicrobial Genetic يبقى
+
+24
+00:02:00,360 --> 00:02:05,900
+علم الـ Microbiology علم واسع بيضم عدة أقسام تحته
+
+25
+00:02:05,900 --> 00:02:11,820
+طبعا إحنا كأخصائي تحاليل طبية هندرس اللي هو الـ
+
+26
+00:02:11,820 --> 00:02:15,000
+Medical Microbiology اللي إيه علاقة في اللي هو
+
+27
+00:02:15,000 --> 00:02:20,820
+الجانب الطبيبالنسبة لـ Medical Microbiology طبعا
+
+28
+00:02:20,820 --> 00:02:26,180
+هو علم كبير وواسع عشان هيك هندرسه في عدة مصاقات
+
+29
+00:02:26,180 --> 00:02:31,700
+خلال سنوات الدراسة ال medical microbiology بضم
+
+30
+00:02:31,700 --> 00:02:36,620
+طبعا أيضا عدة فروع أول فرع اللي هو ال bacteriology
+
+31
+00:02:36,620 --> 00:02:41,340
+هو علم دراسة البكتيريا في هذا المصاق هنركز على
+
+32
+00:02:41,340 --> 00:02:45,840
+دراسة البكتيريا هنتعامل مع البكتيريافي عيننا الـ
+
+33
+00:02:45,840 --> 00:02:51,040
+Virology طبعا الـ Virology هو علم دراسة الفيروسات
+
+34
+00:02:51,040 --> 00:02:56,760
+أخدته في سنة تانية الـ Mycology وهو علم الفطريات و
+
+35
+00:02:56,760 --> 00:03:00,100
+الـ Proto Zoology هو علم الأوليات طبعا هدول
+
+36
+00:03:00,100 --> 00:03:06,040
+العلمين هتاخدوهم في مستوى رابع بالنسبة لـ
+
+37
+00:03:06,040 --> 00:03:10,340
+Immunology وهو علم المناعة علم المناعة كان قديما
+
+38
+00:03:10,340 --> 00:03:15,540
+هو فرع من فروع ال medical microbiologyلكن مع كبر
+
+39
+00:03:15,540 --> 00:03:19,100
+حجم المعلومات في هلم المناعة تم فصله عن ال
+
+40
+00:03:19,100 --> 00:03:25,000
+microbiology أنا بتكلم عن micro organism جسم غريب
+
+41
+00:03:25,000 --> 00:03:30,360
+طبعا antigen دخل جسم الإنسان أكيد هيلاقي رد فعل من
+
+42
+00:03:30,360 --> 00:03:35,340
+جهاز المناعة ويكون له أزام مضادة بالتالي لايمكن
+
+43
+00:03:35,340 --> 00:03:41,080
+فصل ال microbiology عن ال immunology في الدراسة
+
+44
+00:03:43,240 --> 00:03:47,740
+طبعا هك اعرفنا شو تعريف علم ال microbiology شو
+
+45
+00:03:47,740 --> 00:03:51,800
+أقسام علم ال microbiology وعرفنا فروع ال medical
+
+46
+00:03:51,800 --> 00:03:56,860
+microbiology الهدف من قسم ال microbiology في
+
+47
+00:03:56,860 --> 00:04:00,940
+المستشفيات يهدف إلى عزل البكتيريا من العينات
+
+48
+00:04:00,940 --> 00:04:05,880
+المرضية وتشخيصها عبر فحوصات مختلفة طيب احنا
+
+49
+00:04:05,880 --> 00:04:10,840
+هنتعامل مع عينات مرضية يعني احتمال كبير يكون فيها
+
+50
+00:04:10,840 --> 00:04:15,280
+pathogenic microorganismبالتالي إجراءات السلامة
+
+51
+00:04:15,280 --> 00:04:19,500
+والوقاية ضرورية أنه نتبعها في معمل الـ
+
+52
+00:04:19,500 --> 00:04:23,880
+Microbiology عشان هيك في أول أسبوع هيكون في عندنا
+
+53
+00:04:23,880 --> 00:04:28,460
+مقدمة المقدمة هتكون عبارة عن safety rules قواعد
+
+54
+00:04:28,460 --> 00:04:33,240
+السلامة داخل معمل الـ Microbiology وهنتكلم أيضًا
+
+55
+00:04:33,240 --> 00:04:38,380
+عن الأدوات في معمل الـ Microbiology Instrument in
+
+56
+00:04:38,380 --> 00:04:42,620
+Microbiology Lab هنتعرف على الأدوات الخاصة بالمعمل
+
+57
+00:04:43,410 --> 00:04:48,170
+الاسبوع التانى هنتكلم عن ال microscope بالنسبة لها
+
+58
+00:04:48,170 --> 00:04:52,770
+ال microscope احنا نتعامل مع micro organism مع
+
+59
+00:04:52,770 --> 00:04:58,510
+كائنات لتراب العين المجردة بالتالي اكيد انا هحتاج
+
+60
+00:04:58,510 --> 00:05:02,830
+ال microscope حتى ان انا اكبرها و اشوفها بشكل واضح
+
+61
+00:05:03,310 --> 00:05:06,750
+في هذا المعمل هنتعرف على الميكروسكوب أجزاء
+
+62
+00:05:06,750 --> 00:05:12,530
+الميكروسكوب وظيفة كل جزء هنتعرف على كيف أننا
+
+63
+00:05:12,530 --> 00:05:16,610
+نستخدم الميكروسكوب وكيف أقدر أجيب ال field بشكل
+
+64
+00:05:16,610 --> 00:05:22,010
+صحيح بالنسبة للأسبوع التالت والأسبوع الرابع
+
+65
+00:05:22,010 --> 00:05:26,430
+والأسبوع الخامس كلها عبارة عنها تكون عن استان على
+
+66
+00:05:26,430 --> 00:05:32,760
+عن صبغاتفي معمل السوائل اللي أخدته فيه الـ Urine
+
+67
+00:05:32,760 --> 00:05:38,620
+Analysis كان في عندي Microscopic Examination وفي
+
+68
+00:05:38,620 --> 00:05:43,040
+عندي من ضمن ال Microscopic Examination في عندي فرع
+
+69
+00:05:43,040 --> 00:05:50,280
+اللي هو البكتيريا لو أنا طبعا جيبت عينة مريض بالـ
+
+70
+00:05:50,280 --> 00:05:54,120
+UTI اللي هو الـ Urinary Tract Infection الديهاب
+
+71
+00:05:54,120 --> 00:05:58,730
+المسالك البوليةهذا المريض لو جبت عينة يورين وعملت
+
+72
+00:05:58,730 --> 00:06:02,050
+لها centrifugation واخدت طبعا اتخلصت من ال
+
+73
+00:06:02,050 --> 00:06:05,730
+supernatant واخدت ال sediment وحطته على slide و
+
+74
+00:06:05,730 --> 00:06:10,490
+cover slip و تحت الميكروسكوب اشي اكيد هشوف تحت
+
+75
+00:06:10,490 --> 00:06:14,870
+الميكروسكوب في عينة اليورين هشوف بكتيريا هذه
+
+76
+00:06:14,870 --> 00:06:20,070
+البكتيريا هشوفها small حجمها صغير و هشوفها
+
+77
+00:06:20,070 --> 00:06:25,240
+transparent شفافةطبعا البكتيريا transparent شفافة
+
+78
+00:06:25,240 --> 00:06:29,100
+مش هقدر اشوفها بشكل واضح كتير هتحتاج لقوة ملاحظة
+
+79
+00:06:29,100 --> 00:06:33,780
+عشان هيك بلجأ للستان للصبغات حتى اني انا اقدر
+
+80
+00:06:33,780 --> 00:06:38,320
+اشوفها بشكل واضح في عندي انا انواع كتيرة من
+
+81
+00:06:38,320 --> 00:06:42,800
+الصبغات و تصنيفات اول شي ان ال simple stand ال
+
+82
+00:06:42,800 --> 00:06:47,770
+simple stand بشكل مختصرهنحكي عن هتحكي الهدف منها
+
+83
+00:06:47,770 --> 00:06:51,950
+اللي هو اني انا اشوف الشاب و ال arrangement شكل و
+
+84
+00:06:51,950 --> 00:06:56,150
+ترتيب الخلايا الخلية البكتيريا بالنسبة
+
+85
+00:06:56,150 --> 00:06:59,930
+لليجرامستان، ليجرامستان يمكن اسمعتوا فيها بتقسم
+
+86
+00:06:59,930 --> 00:07:05,160
+البكتيريا لمجمعتين موجبة جرام و سالبة جرامالاسد
+
+87
+00:07:05,160 --> 00:07:09,940
+فاستستان هي خاصة ببكتيريا السول الرئوي بكتيريا
+
+88
+00:07:09,940 --> 00:07:12,420
+السول الرئوي اللي هي الـ Mycobacterium
+
+89
+00:07:12,420 --> 00:07:17,380
+tuberculosis هذه البكتيريا ما بقدر اني انا اصبغها
+
+90
+00:07:17,380 --> 00:07:24,860
+بالجرام استان تمام فبالتالي هل جاء للاسد فاستستان
+
+91
+00:07:24,860 --> 00:07:31,200
+لصبغتها طبعا هنعرف ايش السبب في عدم مقدرتنا
+
+92
+00:07:31,200 --> 00:07:36,270
+لصبغتها بالجرام استان في خاصيةفي هذه البكتيريا
+
+93
+00:07:36,270 --> 00:07:41,210
+طبعا تتخلين ما نقدر نصبغها بيه لجرام استان
+
+94
+00:07:41,210 --> 00:07:46,130
+بالأسبوع الخامس فيه اننا هنكمل فيه موضوع الاستان
+
+95
+00:07:46,130 --> 00:07:51,230
+فيه اندي انا تراكيب خاصة في الخلية البكتيرية في
+
+96
+00:07:51,230 --> 00:07:58,360
+هذا المعمل هنصبغ special standالستان خاص هتصبغ
+
+97
+00:07:58,360 --> 00:08:02,260
+تراكيب خاصة داخل الخلية البكتيرية فى عندي الـ
+
+98
+00:08:02,260 --> 00:08:06,280
+Spore Stain اللى هى الأبواغ فى حتى إن أنا أعرف هل
+
+99
+00:08:06,280 --> 00:08:10,680
+البكتيريا قادرة على إنتاج الأبواغ أو لأ بستخدم ال
+
+100
+00:08:10,680 --> 00:08:14,360
+Spore Stain الـ Negative Stain اللى هى اسم آخر
+
+101
+00:08:14,360 --> 00:08:16,740
+اللى هى الـ Capsule Stain حتى إن أنا أعرف
+
+102
+00:08:16,740 --> 00:08:20,760
+البكتيريا هل تمتلك Capsule حافظة أو لأ بصبغها بـ
+
+103
+00:08:20,760 --> 00:08:24,620
+Negative Stainbacterial motility عشان اعرف
+
+104
+00:08:24,620 --> 00:08:29,640
+البكتيريا هل هي motile او non motile بستخدم طبعا
+
+105
+00:08:29,640 --> 00:08:35,560
+اللي هي الفلاجلة استان الفلاجلة يعتبر هو طبعا
+
+106
+00:08:35,560 --> 00:08:39,100
+الجزء المسئول عن الحركة اللي هو الصوت فعشان اعرف
+
+107
+00:08:39,100 --> 00:08:44,480
+هل هي motile أو non motile بصبغ الصوت طبعا هذا
+
+108
+00:08:44,480 --> 00:08:48,080
+بالنسبة لأسبوع التالت والرابع والخامس كلها هتكون
+
+109
+00:08:48,080 --> 00:08:53,660
+عبارة عن استانوهنستخدم اكيد المايكروسكوب فبالتالي
+
+110
+00:08:53,660 --> 00:08:57,320
+لازم نكون طالعين من معمل المايكروسكوب عارفين نجيب
+
+111
+00:08:57,320 --> 00:09:01,480
+ال field لأن كل المعامل هتعتمد على المايكروسكوب
+
+112
+00:09:03,340 --> 00:09:07,360
+في الأسبوع السادس هنبدأ نحكي عن ال isolation عزل
+
+113
+00:09:07,360 --> 00:09:11,380
+البكتيريا طيب انا ليش بدي اعزل بكتيريا احنا قلنا
+
+114
+00:09:11,380 --> 00:09:15,400
+هدفنا في قسم ال microbiology اننا نعزل بكتيريا من
+
+115
+00:09:15,400 --> 00:09:20,500
+عينات مرضية و نشخصها عبر فحوصات مختلفة ايش يعني
+
+116
+00:09:20,500 --> 00:09:24,080
+نشخصها يعني بدي اعطي اسم للبكتيريا المسببة
+
+117
+00:09:24,080 --> 00:09:28,800
+للالتهاف بدي اعرف هويتها اللي على أساسه الدكتور
+
+118
+00:09:28,800 --> 00:09:33,180
+هيوصف هلاجة اللي هو ال antibiotic المناسب للمريض
+
+119
+00:09:33,730 --> 00:09:37,830
+هلأ انا في اول معامل لما أخدت العينة وشوفتها تحت
+
+120
+00:09:37,830 --> 00:09:42,150
+ال microscope وصبغتها فقط عرفت انه انا فيها اندي
+
+121
+00:09:42,150 --> 00:09:45,970
+infection او لأ في بكتيريا في العينة او لأ عرفت
+
+122
+00:09:45,970 --> 00:09:50,290
+معلومات قليلة انه هاي مثلا من اي مجموعة ماقدرت
+
+123
+00:09:50,290 --> 00:09:55,470
+اوصل لاسم البكتيرياعشان هيك لازم انا اعزلها من
+
+124
+00:09:55,470 --> 00:10:00,930
+العيينة عشان الصورة تكون اوضح بدنا انشبه البكتيريا
+
+125
+00:10:00,930 --> 00:10:05,970
+بالجنين والعيينة بالام هلأ ال baby طول ما هو طول
+
+126
+00:10:05,970 --> 00:10:11,390
+ما هو بطن امه فقط بقدر اعرف انه هل الام حامل او لأ
+
+127
+00:10:11,390 --> 00:10:16,010
+هل فيه baby او لأ ممكن اعرف مثلا جنسه جنس الجنين
+
+128
+00:10:16,340 --> 00:10:20,840
+لكن تفاصيل أكتر لون بشرته لون عيونه ما بقدر لازم
+
+129
+00:10:20,840 --> 00:10:26,020
+يولد الطفل و أشوفه حتى أقدر أعرف هذه التفاصيل كذلك
+
+130
+00:10:26,020 --> 00:10:31,040
+البكتيريا لازم أعزلها من العينة و أتعامل معها عشان
+
+131
+00:10:31,040 --> 00:10:36,520
+أعرف هذه التفاصيل و أقدر أشخصها بالنسبة للجنين
+
+132
+00:10:36,520 --> 00:10:42,640
+الجنين حتى يولد و يخرج للحياة لازم يموء و يكبر و
+
+133
+00:10:42,640 --> 00:10:47,840
+بالنهاية يتكون جسم إنسان كاملبرضه الجنين بيحتاج
+
+134
+00:10:47,840 --> 00:10:52,280
+حتى ينمو و يكبر لغذاء هذا الغذاء بياخده من طبعا
+
+135
+00:10:52,280 --> 00:10:57,080
+إمه و لازم يكون في بيئة مناسبة لنموه و بيئة مناسبة
+
+136
+00:10:57,080 --> 00:11:01,960
+لحمايته طبعا هذه البيئة هي رحم إمه هنتعامل مع
+
+137
+00:11:01,960 --> 00:11:06,920
+البكتيريا بنفس تعاملنا مع الجنين حتى نقدر نعزلها
+
+138
+00:11:07,270 --> 00:11:12,210
+أول شئ البكتيريا برضه لازم تنمو و تنقسم طبعا
+
+139
+00:11:12,210 --> 00:11:16,550
+البكتيريا تعتبر unicellular يعني وحيدة الخلية
+
+140
+00:11:16,550 --> 00:11:22,170
+بتنقسم بواسطة الانشطار الثنائي و بتنمو بشكل أُسي و
+
+141
+00:11:22,170 --> 00:11:26,250
+بتكون ملايين من الخلايا هذه الخلايا بتتجمع مع
+
+142
+00:11:26,250 --> 00:11:30,630
+بعضها البعض و بتكون ما يعرف بالكلوني الكلوني هو
+
+143
+00:11:30,630 --> 00:11:36,610
+المستعمر هذه مصطلح الكلونيالـ Colony هي عبارة عن
+
+144
+00:11:36,610 --> 00:11:43,750
+مستعمرة هذا طبعا شكل هذه بنسميها Colony مستعمر هذا
+
+145
+00:11:43,750 --> 00:11:48,810
+الشكل يعتبر Colony تجمع من ملايين من الخلايا
+
+146
+00:11:48,810 --> 00:11:52,810
+البكتيريا تجمعت حوالين بعض و كونت في ما يعرف بال
+
+147
+00:11:52,810 --> 00:11:59,500
+Colony طبعا البكتيريا أناما بقدر أشوفها في الواقع
+
+148
+00:11:59,500 --> 00:12:03,380
+إلا على شكل كولوني زي الجنين ما بقدر نشوفه إلا على
+
+149
+00:12:03,380 --> 00:12:07,760
+شكل جسم إنسان كامل تاني الشيء البكتيريا قلنا إنه
+
+150
+00:12:07,760 --> 00:12:13,540
+حتى تنمو بتحتاج لغذاء الغذاء البكتيريا منسمي media
+
+151
+00:12:15,460 --> 00:12:20,660
+media هو الوسط الغذائي للبكتيريا فده المعمل هنتعرف
+
+152
+00:12:20,660 --> 00:12:24,940
+كيف هنحضر ال media كيف هنحضر الوسط الغذائي
+
+153
+00:12:24,940 --> 00:12:29,520
+للبكتيريا طبعا هذا طبق بطري بالنسبة ل media هي
+
+154
+00:12:29,520 --> 00:12:41,180
+المادة الحمراء تمام هي المادة الحمراء لحظة
+
+155
+00:12:44,740 --> 00:12:48,840
+هذه المادة الحمرة تعتبر هي ال media هنتعرف كيف
+
+156
+00:12:48,840 --> 00:12:53,420
+نحضرها طبعا نصبها في طبق البتري داش بعد كده نجيب
+
+157
+00:12:53,420 --> 00:12:56,660
+العينة اللي أنا شاكه في اندي infection الديهاب
+
+158
+00:12:56,660 --> 00:13:01,760
+بزرع هذه العينة و بحطها في جهاز incubator بعد 24
+
+159
+00:13:01,760 --> 00:13:06,980
+ساعة بشوف نمو البكتيريا على شكل مستعمرة تمام؟ طيب
+
+160
+00:13:07,180 --> 00:13:11,840
+بالنسبة للجنين قلنا الجنين طبعا حاضنته الحاضنة
+
+161
+00:13:11,840 --> 00:13:15,760
+الأساسية هي رحم الأم طول فترة التسعة شهور بيكون في
+
+162
+00:13:15,760 --> 00:13:20,200
+رحم الأم طيب البكتيريا حتى تنمو وتتكثر بدها اللي
+
+163
+00:13:20,200 --> 00:13:24,200
+هي حاضنة حاضنت البكتيريا اسمها incubator هذا
+
+164
+00:13:24,200 --> 00:13:28,560
+الجهاز هنتعرف عليه في أول معمل من معامل الـ
+
+165
+00:13:28,560 --> 00:13:33,220
+microbiologyهذه الـ Incubator هي عبارة عن جهاز
+
+166
+00:13:33,220 --> 00:13:37,740
+بوفر الظروف المناسبة لنمو البكتيريا زي ما رحم الأم
+
+167
+00:13:37,740 --> 00:13:41,400
+بوفر الظروف المناسبة لنمو ال baby و ال incubator
+
+168
+00:13:41,400 --> 00:13:47,990
+بوفر الظروف المناسبة لنمو ال babyالبكتيريا بالنسبة
+
+169
+00:13:47,990 --> 00:13:51,810
+للأسبوع السادس قلنا media preparation and
+
+170
+00:13:51,810 --> 00:13:55,470
+sterilization ال sterilization معناها تعقيم to
+
+171
+00:13:55,470 --> 00:14:00,210
+kill all microorganism حتى أني انا اقتل كل ال
+
+172
+00:14:00,210 --> 00:14:04,090
+microorganism التعقيم ال sterilization في شغل ال
+
+173
+00:14:04,090 --> 00:14:08,650
+microbiology مهم جدا جدا لأن ال microorganism
+
+174
+00:14:08,650 --> 00:14:11,710
+موجودة في كل مكان على الأصدفة فياندي microorganism
+
+175
+00:14:11,710 --> 00:14:16,660
+على الجلد فياندي في الهواء فبالتاليعملية التعقيم
+
+176
+00:14:16,660 --> 00:14:20,720
+مهمة جدا هلأ انا لو حضرت ميديا و خلال تحذيري
+
+177
+00:14:20,720 --> 00:14:25,520
+استخدمت flask ماعقمت هذا ال flask بعدها اجيت صبت
+
+178
+00:14:25,520 --> 00:14:29,380
+الميديا في الطبق اللي بترداش و زرعت عليها عينتي
+
+179
+00:14:29,380 --> 00:14:33,920
+بعد 24 ساعة لاقيت في اندي نمو على الطبق طيب هلأ
+
+180
+00:14:33,920 --> 00:14:39,240
+هذا النمو هل هو من العينة ولا هل هو من ال flask
+
+181
+00:14:39,240 --> 00:14:43,440
+اللي انا ماعقمتهفهنا بيصير عندي في لبس في الموضوع
+
+182
+00:14:43,440 --> 00:14:48,860
+فعشان هي في هذا في شغل ال microbiology لازم عملية
+
+183
+00:14:48,860 --> 00:14:52,200
+تعقيم الأدوات تعقيم الشغل فهذا المعمل هنتعرف كيف
+
+184
+00:14:52,200 --> 00:14:56,860
+بنعقم الأدوات كيف بنعقم مكان الشغل بالنسبالي ال
+
+185
+00:14:56,860 --> 00:15:01,040
+aseptic technique هي جميع الإجراءات اللازمة لمنع
+
+186
+00:15:01,040 --> 00:15:05,260
+ال contamination and pure culture concept مش يعني
+
+187
+00:15:05,260 --> 00:15:10,870
+ابيور ابيور معناها نقي ابيور هي معناها نقيطب عشان
+
+188
+00:15:10,870 --> 00:15:16,230
+نفهم أكتر، نكمل في قصة الجنيد، لو كانت الأم حامل
+
+189
+00:15:16,230 --> 00:15:21,810
+بـ Identical Twins وشأت الأقدار أنه يولدوا متصلين،
+
+190
+00:15:21,810 --> 00:15:25,250
+هدول الـ Twins، هل لازم يكون فيه اندي تدخل ضده
+
+191
+00:15:25,250 --> 00:15:30,110
+لفصل التوقم عن بعض؟ لأنه بالنهاية كل واحد له اسم،
+
+192
+00:15:30,110 --> 00:15:35,280
+كل واحد له شخصيةنفس الاشي بالنسبة للبكتيريا لو كان
+
+193
+00:15:35,280 --> 00:15:40,100
+عندي infection لو كان عندي التهاب وكان سبب الاتهاب
+
+194
+00:15:40,100 --> 00:15:45,280
+أكتر من microorganism هنلاحظ بعد 24 ساعة نمو
+
+195
+00:15:45,280 --> 00:15:49,000
+مستعمرات على الطبق في مستعمرات هتكون مخدة شكل معين
+
+196
+00:15:49,000 --> 00:15:53,080
+ومستعمرات تانية مخدة شكل مختلف هان هحس ان في عندي
+
+197
+00:15:53,080 --> 00:15:56,780
+إذا الكله مش نفس الشكل هعرف ان في عندي two
+
+198
+00:15:56,780 --> 00:16:03,380
+microorganismفلازم هان اخد كل colony كل شكل مثلا
+
+199
+00:16:03,380 --> 00:16:09,000
+الشكل ال colony كل شكل من هدول الشكلين اخد و
+
+200
+00:16:09,000 --> 00:16:13,680
+ازرعهم كل واحدة منهم على طبق على احدى عشان تكون
+
+201
+00:16:13,680 --> 00:16:19,000
+المزرعة عندى pure حتى يكون اصل ال colony عبارة عن
+
+202
+00:16:19,000 --> 00:16:23,740
+خلية بكتيرية واحدة وهذا الاشي ضروري جدا للفحوصات
+
+203
+00:16:23,740 --> 00:16:28,050
+الكيميائيةبالنسبة لآخر إشي الـ Colony Morphology
+
+204
+00:16:28,050 --> 00:16:32,650
+اللي هو شكل المستعمرات على الطبق ممكن من خلال شكل
+
+205
+00:16:32,650 --> 00:16:36,810
+المستعمرات على الطبق أتعرف على اللي هو هوية
+
+206
+00:16:36,810 --> 00:16:40,650
+البكتيريا في بكتيريا عندنا اسمها Streptococcus من
+
+207
+00:16:40,650 --> 00:16:46,550
+مميزات هذه البكتيريا أنه أنا على طبعا شكل
+
+208
+00:16:46,550 --> 00:16:50,370
+المستعمرات هذه البكتيريا على الطبق بتكون pinpoint
+
+209
+00:16:50,370 --> 00:16:54,590
+على شكل راس الدبوسي كتير حجمها صغيرفأنا من حد ما
+
+210
+00:16:54,590 --> 00:16:59,050
+أشوف pinpoint على الطبق احتمالي كبير بحكي انه high
+
+211
+00:16:59,050 --> 00:17:04,070
+streptolacin لازم نأكد ال .. طبعا نأكد اللي هو
+
+212
+00:17:04,070 --> 00:17:12,230
+التشخيص بفكوثات كيميائية هذا بالنسبة لأسبوع السادس
+
diff --git a/PL9fwy3NUQKwZWgAb_V1ATlRRsH-6i3cPC/fiNRLFNYLh8.srt b/PL9fwy3NUQKwZWgAb_V1ATlRRsH-6i3cPC/fiNRLFNYLh8.srt
new file mode 100644
index 0000000000000000000000000000000000000000..e739f81930fdc1ebea1564e29d9f220396e0121c
--- /dev/null
+++ b/PL9fwy3NUQKwZWgAb_V1ATlRRsH-6i3cPC/fiNRLFNYLh8.srt
@@ -0,0 +1,1294 @@
+1
+00:00:02,330 --> 00:00:06,610
+Methyl red Voges-Proskauer test هذا الفحص له علاقة
+
+2
+00:00:06,610 --> 00:00:09,990
+بالبكتيريا الـ fermentation لو البكتيريا عملت
+
+3
+00:00:09,990 --> 00:00:14,710
+fermentation للجليكوز، هعمل فحص الـ Methyl red Voges-Proskauer
+
+4
+00:00:14,710 --> 00:00:18,950
+test. قلنا أن من نتائج عملية الـ fermentation
+
+5
+00:00:18,950 --> 00:00:24,210
+الـ end product إما يكون أسيد أو كحول، وقلنا أن الـ
+
+6
+00:00:24,210 --> 00:00:27,210
+end product هي من خلالها أنا بقدر أعمل
+
+7
+00:00:27,210 --> 00:00:30,570
+identification للبكتيريا. كيف بدي أعمل
+
+8
+00:00:30,570 --> 00:00:33,890
+identification للبكتيريا من خلال الـ end product
+
+9
+00:00:33,890 --> 00:00:38,470
+من خلال فحص الـ methyl red Voges-Proskauer test عشان نعرف
+
+10
+00:00:38,470 --> 00:00:49,090
+أكثر لو
+
+11
+00:00:49,090 --> 00:00:52,810
+البكتيريا عملت fermentation للجليكوز، عملت فحص
+
+12
+00:00:52,810 --> 00:00:57,070
+البكتيريا الـ fermentation اللي شرحناه في الجزء
+
+13
+00:00:57,070 --> 00:01:01,580
+الأول، بيقول لي بتعمل فحص الـ Methyl Red Voges-Proskauer
+
+14
+00:01:01,580 --> 00:01:05,160
+test. الـ Methyl Red Voges-Proskauer test
+
+15
+00:01:05,160 --> 00:01:09,980
+هذا من خلاله بدي أعرف أي pathway سلكت البكتيريا.
+
+16
+00:01:09,980 --> 00:01:15,580
+البكتيريا لما تنتج acid، بيقول لي بتسلك الـ pathway
+
+17
+00:01:15,580 --> 00:01:19,600
+اسمه mixed acid fermentation. يبقى لو كان ناتج
+
+18
+00:01:19,600 --> 00:01:24,480
+عملية الـ fermentation acid، بتكون البكتيريا سلكت الـ
+
+19
+00:01:24,480 --> 00:01:28,870
+pathway اللي اسمه mixed acid fermentation. لو كان
+
+20
+00:01:28,870 --> 00:01:33,070
+ناتج عملية الـ fermentation كحول، بتكون البكتيريا
+
+21
+00:01:33,070 --> 00:01:38,470
+سلكت الـ pathway اللي اسمه butylene glycol pathway
+
+22
+00:01:38,470 --> 00:01:44,750
+أو neutral pathway أو butanediol pathway. ثلاث أسماء
+
+23
+00:01:44,750 --> 00:01:51,510
+لـ pathway هذا، اللي نواتج الـ fermentation بتكون
+
+24
+00:01:51,510 --> 00:01:56,480
+كحول. بالنسبة لـ mixed acid fermentation، أنا
+
+25
+00:01:56,480 --> 00:02:01,020
+عشان أكشف هل البكتيريا سلكت هذا الـ pathway، بستخدم
+
+26
+00:02:01,020 --> 00:02:06,560
+فحص الـ Methyl Red Test عشان أعرف البكتيريا سلكت
+
+27
+00:02:06,560 --> 00:02:10,940
+الـ pathway اللي اسمه neutral pathway أو بيوتانديول
+
+28
+00:02:10,940 --> 00:02:16,580
+أو بيوتيلين. أيش اللي بيقول pathway؟ بستخدم فحص الـ
+
+29
+00:02:16,580 --> 00:02:20,640
+Voges-Proskauer test. بالنسبة لـMixed Acid
+
+30
+00:02:20,640 --> 00:02:23,980
+Fermentation، قلنا أن البكتيريا بتعمل ferment
+
+31
+00:02:23,980 --> 00:02:29,280
+ للجليكوز، and produce extreme amount of acid. قلنا
+
+32
+00:02:29,280 --> 00:02:32,720
+نواتج العملية في هذا الـ pathway acid. بقول extreme
+
+33
+00:02:32,720 --> 00:02:37,460
+amount of acid. بتكون الـ acid strong acid زي الـ
+
+34
+00:02:37,460 --> 00:02:41,160
+formic acid، الـ acetic acid، و الـ lactic acid. هاي الـ
+
+35
+00:02:41,160 --> 00:02:46,060
+acid تعتبر strong acid. فبالتالي هتعمل على خفض الـ
+
+36
+00:02:46,060 --> 00:02:52,140
+pH بشكل كبير. high decrease in pH. أقل من خمسة الـ pH
+
+37
+00:02:52,140 --> 00:02:57,500
+بوصل. فبالتالي بيعمل على تغيير لون الـ media في
+
+38
+00:02:57,500 --> 00:03:02,040
+الفحص. الـ bacterial fermentation لو عملته بشكل كبير
+
+39
+00:03:02,040 --> 00:03:06,380
+بيعمل على تغيير الـ pH، بيخفض الـ pH بشكل كبير.
+
+40
+00:03:06,380 --> 00:03:10,160
+عشان هيك بفحص الـ bacterial fermentation اللي حكينا
+
+41
+00:03:10,160 --> 00:03:16,320
+عنه، كان في تيوب لونها، لون التيوب كان أصفر غامق، أصفر
+
+42
+00:03:16,320 --> 00:03:20,380
+غامق. قلنا لأنه بتنتج كمية كبيرة من الـ acid، strong
+
+43
+00:03:20,380 --> 00:03:24,780
+acid. يبقى كانت في تيوب هاي الـ pathway mixed acid
+
+44
+00:03:24,780 --> 00:03:29,320
+pathway. كان extreme amount of acid. بيقول لي نواتج
+
+45
+00:03:29,320 --> 00:03:33,000
+عملية الـ fermentation في الـ mixed acid
+
+46
+00:03:33,000 --> 00:03:38,300
+fermentation بتكون stable. عشان أكشف عن هاي الـ
+
+47
+00:03:38,300 --> 00:03:42,720
+pathway، بضيف reagent اسمه methyl red reagent.
+
+48
+00:03:42,720 --> 00:03:45,780
+هنتعرف على الـ procedure ومتى أنا هضيفها. ده الـ
+
+49
+00:03:45,780 --> 00:03:51,380
+methyl red. بالنسبة لـ neutral pathway أو
+
+50
+00:03:51,380 --> 00:03:55,080
+butanediol pathway أو butylene glycol pathway،
+
+51
+00:03:55,080 --> 00:03:58,820
+بيقول لي أن البكتيريا بتعمل fermentation للجليكوز،
+
+52
+00:03:58,820 --> 00:04:04,840
+لكن بتنتج الكحول اللي هو الأسيتون. الأسيتون يعتبر
+
+53
+00:04:04,840 --> 00:04:09,180
+الكحول من نواتج عملية الـ fermentation. هذا الأسيتون
+
+54
+00:04:09,180 --> 00:04:14,060
+يعتبر unstable على عكس الـ strong acid. كانت هنا في
+
+55
+00:04:14,060 --> 00:04:18,060
+الـ mixed acid pathway stable، لكن الأسيتون يعتبر
+
+56
+00:04:18,060 --> 00:04:23,200
+unstable. acidic product، أو هو يعتبر acidic لكن مش
+
+57
+00:04:23,200 --> 00:04:27,380
+strong acid. هيعمل على خفض الـ pH لكن مش بصورة كبيرة.
+
+58
+00:04:27,380 --> 00:04:32,460
+هيكون slight decrease in pH. بيقول لي بما أنه
+
+59
+00:04:32,460 --> 00:04:38,620
+unstable، أكيد عشان يصير مستقر هيتحول لمركب أكثر
+
+60
+00:04:38,620 --> 00:04:43,920
+استقراراً، هيتحول لبيوتان ديول. هذا البيوتان ديول
+
+61
+00:04:43,920 --> 00:04:48,640
+stable. عشان هيك سميت الـ pathway اسمه بيوتان ديول
+
+62
+00:04:48,640 --> 00:04:52,960
+pathway. هذا المركب بيقول لي neutral product، عشان
+
+63
+00:04:52,960 --> 00:04:55,900
+هيك سميت الـ pathway اسم ثاني، كان الـ neutral
+
+64
+00:04:55,900 --> 00:04:59,880
+pathway. يبقى هيعمل لي slight decrease in pH، عشان
+
+65
+00:04:59,880 --> 00:05:04,960
+هيك الـ tube كانت في الجزء قبل في المحاضرة لما
+
+66
+00:05:04,960 --> 00:05:09,220
+كشفت عن الـ fermentation، كان اللون فاتح. اتكون
+
+67
+00:05:09,220 --> 00:05:13,100
+الكحول، عمل لي على خفض الـ pH، لكن كان slight decrease
+
+68
+00:05:13,100 --> 00:05:19,910
+in pH. عشان أوضح الصورة أكثر أو أن أنا أعرف هل هي
+
+69
+00:05:19,910 --> 00:05:23,990
+سلكت الـ Voges-Proskauer pathway اللي هو الـ butanediol pathway
+
+70
+00:05:23,990 --> 00:05:27,510
+أو الـ mixed acid pathway، لازم أعمل فحص
+
+71
+00:05:27,510 --> 00:05:30,930
+الـ methyl red Voges-Proskauer، لأن أوقات ممكن ما يكون في
+
+72
+00:05:30,930 --> 00:05:34,930
+عندي الـ tube أن أنا أقارن الفرق في اللون، هل هو
+
+73
+00:05:34,930 --> 00:05:38,110
+غامق أو فاتح. ممكن تطلع كلها البكتيريا اللي عندي
+
+74
+00:05:38,110 --> 00:05:41,910
+سلكت طريقة الـ mixed acid pathway، فبالتالي مش هقدر
+
+75
+00:05:41,910 --> 00:05:45,310
+أن أنا أفرد. فعشان هيك عملوا فحص الـ Methyl Red
+
+76
+00:05:45,310 --> 00:05:49,550
+Voges-Proskauer. الـ reagent اللي أنا بدي أضيفه عشان
+
+77
+00:05:49,550 --> 00:05:53,230
+أكشف على هذا الـ pathway اسمه Barritt's Reagent. الـ
+
+78
+00:05:53,230 --> 00:05:57,870
+Barritt's Reagent بتكون من VP1، VP2. نتيجة اختصار لـ
+
+79
+00:05:57,870 --> 00:06:02,270
+الـ V اسم الفحص اللي هو Voges-Proskauer. الأول الـ
+
+80
+00:06:02,270 --> 00:06:05,070
+reagent اللي أنا بضيفه اللي هو خمسة في المئة Alpha-
+
+81
+00:06:05,070 --> 00:06:10,310
+naphthol. VP2 اللي بدي أضيفه بعد ما ضفت الأول، هضيف
+
+82
+00:06:10,310 --> 00:06:17,770
+reagent أربعين في المئة KOH. KOH هو هذا الـ VP2. يبقى
+
+83
+00:06:17,770 --> 00:06:22,110
+الـ Barritt's reagent بتكون من VP1 و VP2. الـ VP1 Alpha-
+
+84
+00:06:22,110 --> 00:06:28,110
+naphthol. الـ VP2 أربعين في المئة KOH. طيب كيف أنا
+
+85
+00:06:28,110 --> 00:06:34,330
+بدي أكشف على الـ pathway، أي pathway سلكت البكتيريا؟
+
+86
+00:06:34,330 --> 00:06:40,110
+بيقول لي أني بدي أجيب media اسمها methyl red Voges-Proskauer
+
+87
+00:06:40,110 --> 00:06:43,090
+medium. هاي الـ media بتكون broth. الميديا
+
+88
+00:06:43,090 --> 00:06:48,390
+بتحتوي على جليكوز، أكيد كـ substrate، هيشتغل عليها
+
+89
+00:06:48,390 --> 00:06:52,790
+هتشتغل عليها البكتيريا، هتعمل fermentation للجليكوز.
+
+90
+00:06:52,790 --> 00:06:58,410
+في عندي في الميديا برضه، مصدر للـ nitrogen
+
+91
+00:06:58,410 --> 00:07:02,550
+source عشان تنمو البكتيريا. بولي هاي الـ media
+
+92
+00:07:02,550 --> 00:07:07,470
+بتاخدي two tube منها. واحد الـ tube هيكون لفحص الـ
+
+93
+00:07:07,470 --> 00:07:11,250
+methyl red test، و الـ tube الثاني هيكون لفحص الـ VP
+
+94
+00:07:11,250 --> 00:07:17,980
+test. نفس الـ substrates، لأن نفس الـ substrates هيشتغلوا عليها
+
+95
+00:07:17,980 --> 00:07:22,420
+فبالتالي هتكون نفس الـ media في كلا الفحصين، لكن
+
+96
+00:07:22,420 --> 00:07:26,040
+الاختلاف في الـ reagent اللي أنا هضيفه بعد الـ
+
+97
+00:07:26,040 --> 00:07:29,380
+incubation. بعد الـ incubation، لفحص الـ methyl red
+
+98
+00:07:29,380 --> 00:07:32,580
+هضيف الـ reagent اللي هو قلنا methyl red reagent.
+
+99
+00:07:32,580 --> 00:07:36,540
+لكن لفحص الـ Voges-Proskauer test، هضيف الـ Barritt's
+
+100
+00:07:36,540 --> 00:07:41,820
+reagent. يبقى أنا بستخدم هذول الفحصين باستخدام لهم
+
+101
+00:07:41,820 --> 00:07:45,120
+نفس الـ media. هاي الـ media بتحتوي على جليكوز كـ
+
+102
+00:07:45,120 --> 00:07:49,120
+substrate. بيقول لي نفس الـ organism بدي أزرعه في
+
+103
+00:07:49,120 --> 00:07:53,680
+توب تيوب. هعمل incubation لمدة 24 ساعة على درجة
+
+104
+00:07:53,680 --> 00:07:57,760
+حرارة 37. بعد ما تخلص فترة الـ incubation وتمّ
+
+105
+00:07:57,760 --> 00:08:02,820
+البكتيريا، أكيد هتكون عاملة fermentation للجليكوز.
+
+106
+00:08:02,820 --> 00:08:06,420
+تمام. أنا بالشكل المبدأي عرفت أنه هي جليكوز
+
+107
+00:08:06,420 --> 00:08:10,100
+fermenter من الفحص اللي حكينا عنه في الجزء الأول.
+
+108
+00:08:10,100 --> 00:08:13,540
+هلا بدي أعرف أي pathway سلكت، الـ mixed acid pathway
+
+109
+00:08:13,540 --> 00:08:16,000
+أو بيوتان بيوتان بيوتان بيوتان بيوتان بيوتان
+
+110
+00:08:16,000 --> 00:08:17,280
+بيوتان بيوتان بيوتان بيوتان بيوتان بيوتان بيوتان
+
+111
+00:08:17,280 --> 00:08:24,760
+بيوتان بيوتان بيوتان بيوتان بيوتان بيوتان بيوتان
+
+112
+00:08:24,760 --> 00:08:27,780
+بيوتان بيوتان بيوتان بيوتان بيوتان بيوتان بيوتان
+
+113
+00:08:27,780 --> 00:08:32,120
+بيوتان بيوتان بيوتان بيوتان بيوتان بيوتان بيوتان
+
+114
+00:08:32,120 --> 00:08:37,590
+بيعمل على خفض الـ pH بصورة كبيرة. high decrease in
+
+115
+00:08:37,590 --> 00:08:42,590
+pH. هضيف أكيد pH indicator. بيقول الـ pH indicator في
+
+116
+00:08:42,590 --> 00:08:47,990
+هذا الفحص هو الـ methyl red. مش بيكون موجود في الـ
+
+117
+00:08:47,990 --> 00:08:51,430
+media. أنا بضيفه بعد ما أخلص incubation، بعد ما
+
+118
+00:08:51,430 --> 00:08:54,570
+أخلص فترة الـ incubation، بضيف الـ pH indicator.
+
+119
+00:08:54,570 --> 00:08:59,270
+بيكون الـ reagent لونه أحمر. بضيف two drop من one لـ
+
+120
+00:08:59,270 --> 00:09:05,210
+three drop على الـ tube. الميثايل ريد ريآجنت شو حدوده؟
+
+121
+00:09:05,210 --> 00:09:09,610
+بيقول لي بيكون في الـ acidic لونه أحمر، وفي الـ basic
+
+122
+00:09:09,610 --> 00:09:14,170
+بيكون لونه أصفر. لنيوترال بيكون ما بين 4 و 4.6 و
+
+123
+00:09:14,170 --> 00:09:18,570
+2، لونه orange. ننتبه، بالفحص اللي اتكلمنا عنه بالأول،
+
+124
+00:09:18,570 --> 00:09:21,090
+الـ fermentation، عشان أشوف بشكل عام هل هي بتعمل
+
+125
+00:09:21,090 --> 00:09:26,510
+fermentation ولا لأ، كان الـ pH Indicator في methyl red
+
+126
+00:09:26,510 --> 00:09:28,770
+وكان موجود في الـ media. أنا ما ضفته بعد الـ
+
+127
+00:09:28,770 --> 00:09:32,670
+incubation. الحدود معاكسة. كانت هناك في الـ acidic
+
+128
+00:09:32,670 --> 00:09:37,130
+yellow، وفي الـ basic لون red. لكن هنا في الـ acidic
+
+129
+00:09:37,130 --> 00:09:42,230
+لونه أحمر، وفي الـ basic لون yellow. يبقى اتكون عندي
+
+130
+00:09:42,230 --> 00:09:46,710
+من نتيجة الـ fermentation strong acid، هتعمل على خفض
+
+131
+00:09:46,710 --> 00:09:53,960
+الـ pH بصورة كبيرة. هتكون أقل من 4.4. هيتحول اللون
+
+132
+00:09:53,960 --> 00:09:57,120
+اللون للون red. يبقى النتيجة الـ positive هان red.
+
+133
+00:09:57,120 --> 00:10:00,180
+كانت هناك في الـ fermentation بالبكتيريا الـ
+
+134
+00:10:00,180 --> 00:10:03,920
+fermentation كانت النتيجة الـ yellow كانت هي الـ
+
+135
+00:10:03,920 --> 00:10:09,180
+positive، لأن الـ indicator بيختلف. لون الـ media
+
+136
+00:10:09,180 --> 00:10:13,700
+لونها أصفر اللي هنزرع عليها. فلو ما تغير أي شيء ضل
+
+137
+00:10:13,700 --> 00:10:16,760
+لون الـ media لونه yellow، يبقى هي negative. من
+
+138
+00:10:16,760 --> 00:10:20,620
+الأمثلة على الـ positive، الـ E.coli و الـ Proteus mirabilis.
+
+139
+00:10:20,620 --> 00:10:24,350
+من الأمثلة على الـ negative،
+
+140
+00:10:24,350 --> 00:10:27,970
+الـ Klebsiella pneumoniae و الـ Enterobacter. يبقى هذا
+
+141
+00:10:27,970 --> 00:10:32,390
+الفحص الـ Methyl Red Voges-Proskauer يستخدم لتفريق
+
+142
+00:10:32,390 --> 00:10:36,010
+الكوليفورم بكتيريا. قلنا الكوليفورم بكتيريا اللي هي
+
+143
+00:10:36,010 --> 00:10:40,110
+الـ E.coli، الـ Citrobacter، الـ Enterobacter، و
+
+144
+00:10:40,110 --> 00:10:43,350
+الـ Klebsiella. عشان أفرق بينهم، أخذنا فحص السيطرات.
+
+145
+00:10:43,350 --> 00:10:46,770
+بيقول لي برضه بتستخدمي فحص الـ Methyl Red Voges-Proskauer
+
+146
+00:10:46,770 --> 00:10:51,350
+و هنستخدم برضه فحص الاندول. هذا فيما يسمى بـ IMViC.
+
+147
+00:10:51,660 --> 00:10:55,020
+الـ IMViC استخدم لتمييز الكوليفورم. بش تغير
+
+148
+00:10:55,020 --> 00:10:58,420
+الأربع فحوصات عشان أفرق الكوليفورم بكتيريا
+
+149
+00:10:58,420 --> 00:11:01,240
+الـ Klebsiella، الـ Citrobacter، الـ Enterobacter، و الـ E.coli.
+
+150
+00:11:01,240 --> 00:11:05,760
+هذا بالنسبة لـ Methyl Red. بالنسبة للـ Voges-
+
+151
+00:11:05,760 --> 00:11:08,840
+Proskauer Test. بعد ما أنا أعمل incubation و أخلص
+
+152
+00:11:08,840 --> 00:11:12,520
+فترة الـ incubation، عشان أكشف عنه بضيف الـ Barritt's
+
+153
+00:11:12,520 --> 00:11:15,900
+reagent. نفس الفكرة. هضيف الـ Barritt's reagent بعد الـ
+
+154
+00:11:15,900 --> 00:11:21,480
+incubation، وبيقول لي بتضيفي VP1 بعدين بتضيفي VP2. ممنوع
+
+155
+00:11:21,480 --> 00:11:27,160
+أنا أبدل. هنحكي السبب بعد ما نشرح كيف أو إيش
+
+156
+00:11:27,160 --> 00:11:32,780
+التفاعل اللي بيصير. قلنا من نواتج عملية الـ
+
+157
+00:11:32,780 --> 00:11:36,400
+pathway اللي هو الـ butanediol pathway، أسيتوين.
+
+158
+00:11:36,400 --> 00:11:40,760
+الأسيتون هو كحول. قلنا الكحول، الأمثلة عليها
+
+159
+00:11:40,760 --> 00:11:45,520
+الأسيتون. الأسيتون يعتبر acidic لكن ما بيعمل على
+
+160
+00:11:45,520 --> 00:11:51,000
+خفض الـ pH بصورة كبيرة. Unstable. Reagent VP1 اللي هو
+
+161
+00:11:51,000 --> 00:11:55,000
+الـ Alpha-naphthol بيرتبط في الـ acetone وبيعطيني
+
+162
+00:11:55,000 --> 00:11:59,280
+complex. بعدها أنا هضيف، بعد مدة تقريباً 10 دقائق،
+
+163
+00:11:59,280 --> 00:12:06,240
+هضيف الـ VP2. الـ VP2 اللي هو الـ 40% KOH. الـ KOH
+
+164
+00:12:06,240 --> 00:12:10,940
+الـ KOH هيرتبط بهذا الـ complex ويعطيني اللون الـ red.
+
+165
+00:12:10,940 --> 00:12:15,580
+يبقى النتيجة الـ positive هتكون red، لكن النتيجة
+
+166
+00:12:15,580 --> 00:12:18,000
+الـ negative مش هيصير تغير في الميديا. لون الميديا
+
+167
+00:12:18,000 --> 00:12:22,560
+لونها أصفر. البكتيريا الـ positive لهذا الفحص
+
+168
+00:12:22,560 --> 00:12:25,480
+Klebsiella pneumoniae و Enterobacter. لكن الـ negative
+
+169
+00:12:25,480 --> 00:12:29,520
+الـ E.coli و الـ Proteus mirabilis. نلاحظ أن اللي
+
+170
+00:12:29,520 --> 00:12:33,580
+كانت positive في الـ methyl red هتكون negative في
+
+171
+00:12:33,580 --> 00:12:36,180
+الـ Voges-Proskauer، واللي كانت negative في الـ
+
+172
+00:12:36,180 --> 00:12:40,800
+methyl red هتكون positive في الـ
+
+223
+00:16:28,810 --> 00:16:32,610
+Acetone لأنه تحول إلى Butanediol، فيعطيني negative
+
+224
+00:16:32,610 --> 00:16:35,550
+result  لن يتغير اللون إلى Red، سيكون not changed
+
+225
+00:16:35,550 --> 00:16:39,350
+في اللون، الميديا، فبالتالي يعطي false negative
+
+226
+00:16:39,350 --> 00:16:45,150
+result لذلك، يجب أن تكون المزرعة مأخوذة أو
+
+227
+00:16:45,150 --> 00:16:50,850
+أُضيف الـReagent بعد 24 ساعة، لا تزيد عن 2، وليس بعد 72
+
+228
+00:16:50,850 --> 00:16:55,450
+ساعة، لأن الـAcetone غير مستقر (unstable)؛ سيتحول إلى Butanediol
+
+229
+00:16:55,450 --> 00:16:59,270
+والـReagent يرتبط فقط مع الـAcetone وليس مع
+
+230
+00:16:59,270 --> 00:17:07,170
+الـButanediol، فيعطيني False Negative Result. طيب،
+
+231
+00:17:07,640 --> 00:17:10,380
+قلنا لو البكتيريا قامت بعملية تخمير (fermentation) للجلوكوز (Gelicose)
+
+232
+00:17:10,380 --> 00:17:12,740
+وقمتُ بفحص البكتيريا لعملية التخمير (fermentation) التي تكلمنا
+
+233
+00:17:12,740 --> 00:17:16,680
+عنها في الجزء الأول، وهو الذي فيه اللون الأحمر (Red)
+
+234
+00:17:16,680 --> 00:17:21,340
+وGelicose والأنبوب ذو المِجرى (Durham Tube). هذا الفحص، لو قمتُ به
+
+235
+00:17:21,340 --> 00:17:26,540
+وطلعت النتيجة موجبة (positive)، أقوم بفحص الـmixed methyl red Voges-Proskauer
+
+236
+00:17:26,540 --> 00:17:31,660
+test. لو طلعت methyl red موجبة (positive) و
+
+237
+00:17:31,660 --> 00:17:34,580
+Voges-Proskauer سالبة (negative)، فهي تكون قد سلكت المسار (pathway)
+
+238
+00:17:34,580 --> 00:17:37,720
+المختلط للحوامض (mixed acid pathway)، وهو ناتج عملية
+
+239
+00:17:37,720 --> 00:17:42,160
+التخمير (fermentation) لحمض قوي. من الأمثلة على هذا
+
+240
+00:17:42,160 --> 00:17:46,940
+الموجب (positive) للميثيل ريد (methyl red) والسالب (negative) لفوجس-بروكساور (Voges-Proskauer)  هي  E.
+
+241
+00:17:46,940 --> 00:17:52,620
+coli. وقلنا أيضاً لبكتيريا المِرَابِيلِس (Proteus mirabilis). لكن لو كانت
+
+242
+00:17:52,620 --> 00:17:55,940
+Methyl Red سالبة (Negative) و Voges-Proskauer موجبة (Positive)، يعني
+
+243
+00:17:55,940 --> 00:18:00,560
+سلكت مسار البيوتيلين غليكول (butylene glycol pathway). من الأمثلة
+
+244
+00:18:00,560 --> 00:18:04,820
+على ذلك أن تكون Methyl Red سالبة (Negative)، Voges-Proskauer
+
+245
+00:18:04,820 --> 00:18:08,370
+موجبة (Positive) مثل  السالمونيلا (Salmonella) وبعض البكتيريا الأخرى. أقول لكم، لو
+
+246
+00:18:08,370 --> 00:18:12,210
+البكتيريا لم تقم بعملية التخمير (fermentation) أصلاً للجلوكوز، أنا
+
+247
+00:18:12,210 --> 00:18:15,250
+ممنوع أن أقوم أصلاً بفحص الـ Methyl Red-Voges-Proskauer
+
+248
+00:18:15,250 --> 00:18:19,910
+test لأن لو قمتُ بالفحص، فستكون النتيجة
+
+249
+00:18:19,910 --> 00:18:23,390
+سالبة (negative)  لـ Methyl Red وسالبة (negative) لـ Voges-Proskauer
+
+250
+00:18:23,390 --> 00:18:26,770
+test. مثل السيودوموناس (Pseudomonas)، السيودوموناس أصلاً لا تقوم ب
+
+251
+00:18:26,770 --> 00:18:29,630
+عملية تخمير (fermentation) للجلوكوز، فلا سيتكون حامض، ولا
+
+252
+00:18:29,630 --> 00:18:34,090
+سيتكون كحول. ممنوع أن أرى نتيجة أن الإثنين
+
+253
+00:18:34,090 --> 00:18:36,370
+موجبان (positive)  لـ Methyl Red و Voges-Proskauer test لو لم
+
+254
+00:18:36,370 --> 00:18:40,170
+أقوم بهما لنفس الكائن الحي، ممنوع أن يكون الاثنان
+
+255
+00:18:40,170 --> 00:18:44,190
+موجبان (positive)، يجب أن يكون أحدهما موجباً (positive) والآخر سالباً (negative)
+
+256
+00:18:44,190 --> 00:18:47,850
+أو كلاهما سالبان (negative) لو لم تكن تقوم أصلاً ب
+
+257
+00:18:47,850 --> 00:18:50,750
+عملية تخمير (Gelicose fermentation) للجلوكوز. هذا ممنوع أن أراه، لأن
+
+258
+00:18:50,750 --> 00:18:54,910
+البكتيريا إما تسلك مسار الأحماض المختلطة (mixed acid pathway) أو
+
+259
+00:18:54,910 --> 00:18:59,910
+مسار البيوتيلين غليكول (butylene glycol pathway)، لا تسلك الاثنين معاً.
+
+260
+00:19:03,410 --> 00:19:07,350
+إذن، لو طرحنا سؤالاً بأن فحص (Voges-Proskauer) Reagent  يستخدم ل
+
+261
+00:19:07,350 --> 00:19:11,670
+الكشف عما إذا كانت البكتيريا تقوم بعملية تخمير (fermentation) في
+
+262
+00:19:11,670 --> 00:19:16,250
+مسار الحامض (acid pathway) أم لا، فنقول  خطأ (False). فحص (Voges-Proskauer) الذي أستخدمه
+
+263
+00:19:16,250 --> 00:19:21,130
+لأرى ما إذا كانت البكتيريا قد سلكت أو لم تسلك مسار الحمض (acid pathway)، أستخدم
+
+264
+00:19:21,130 --> 00:19:25,370
+فحص الميثيل ريد (methyl red) Voges-Proskauer Reagent.
+
+265
+00:19:25,370 --> 00:19:31,190
+أما في مسار البيوتانديول (butanediol pathway)، فكما قلنا أن
+
+266
+00:19:31,190 --> 00:19:35,300
+هذا ممنوع أن أراه. الوسط نفسه (Media) لفحص الـ
+
+267
+00:19:35,300 --> 00:19:38,280
+Methyl red و الـ Voges-Proskauer، لكن الاختلاف في الـ
+
+268
+00:19:38,280 --> 00:19:44,220
+Reagent. الـ Reagent  يكون في مسار الأحماض المختلطة (Mixed Acid Pathway) الـ Methyl red، في مسار البيوتيلين
+
+269
+00:19:44,220 --> 00:19:47,560
+البيوتيلين غليكول (butylene glycol).  البيوتيلينيش
+
+270
+00:19:47,560 --> 00:19:47,860
+البيوتيلينيش
+
+271
+00:19:47,860 --> 00:19:48,440
+البيوتيلينيش
+
+272
+00:19:48,440 --> 00:19:48,660
+البيوتيلينيش
+
+273
+00:19:48,660 --> 00:19:50,420
+البيوتيلينيش
+
+274
+00:19:50,420 --> 00:19:52,220
+البيوتيلينيش
+
+275
+00:19:52,220 --> 00:19:57,860
+البيوتيلينيش
+
+276
+00:19:57,860 --> 00:20:00,820
+البيوتيلين
+
+277
+00:20:01,770 --> 00:20:05,550
+لو رأيتُ قمتُ بالفحص وأضفتُ  Voges-Proskauer reagent، ستكون
+
+278
+00:20:05,550 --> 00:20:09,690
+موجبة (positive) لفحص فوجس-بروكساور (Voges-Proskauer)، لكن فحص التخمير (fermentation)
+
+279
+00:20:09,690 --> 00:20:14,480
+قلنا النتيجة الموجبة (positive) يكون لونها أصفر (yellow). هكذا نكون
+
+280
+00:20:14,480 --> 00:20:18,000
+قد أنهينا تقريباً أهم الأشياء التي يجب أن نعرفها في فحص
+
+281
+00:20:18,000 --> 00:20:22,240
+الميثيل ريد (methyl red) وفوجس-بروكساور (Voges-Proskauer). سننتقل إلى شرائح (slides) نمر عليها
+
+282
+00:20:22,240 --> 00:20:26,260
+أقول، فحص الميثيل ريد (methyl red): بعض البكتيريا الشكلية (coliform) ستُخمر
+
+283
+00:20:26,260 --> 00:20:30,200
+(ferment)  ما تستخلص من الدكستروز (dextrose) وهو الجلوكوز (glucose) إلى
+
+284
+00:20:30,200 --> 00:20:33,720
+منتجات حمضية (acid product) التي ستؤدي إلى انخفاض مستوى  pH إلى أقل من
+
+285
+00:20:33,720 --> 00:20:38,040
+5. يسمى هذا تخمير (fermentation) مختلط للحوامض (mixed acid fermentation). قلنا
+
+286
+00:20:38,040 --> 00:20:41,280
+أن بعض البكتيريا الشكلية (coliform) وليس كل البكتيريا…  الكوليفورم
+
+287
+00:20:41,280 --> 00:20:45,080
+تقوم بعملية تخمير (fermentation)  للدكستروز (dextrose) إلى أحماض قوية (Strong Acid)
+
+288
+00:20:45,080 --> 00:20:49,480
+فقط E.coli من الكوليفورم تكون موجبة (positive)
+
+289
+00:20:49,480 --> 00:20:53,720
+للميثيل ريد (methyl red) ؛ تسلك مسار تخمير الأحماض المختلطة (Mixed Acid Fermentation)
+
+290
+00:20:53,720 --> 00:20:57,800
+هذا سينتج أحماض قوية (Strong Acid)  سوف تؤثر على مستوى pH بشكل
+
+291
+00:20:57,800 --> 00:21:03,760
+كبير (high decrease in pH) إلى أقل من خمسة، حتى أقل
+
+292
+00:21:03,760 --> 00:21:08,260
+من 4.4 بعد الحضانة (incubation). أضيف مَن؟ أضيف
+
+293
+00:21:08,260 --> 00:21:11,700
+كاشف (reaction) الميثيل ريد (methyl red) لفحص  تخمير الأحماض المختلطة (Mixed Acid Fermentation).
+
+294
+00:21:11,700 --> 00:21:15,670
+هذا الكاشف (Methyl Red) سيُحوّل لونه إلى
+
+295
+00:21:15,670 --> 00:21:21,290
+أحمر في حال كان هناك منتجات حمضية (acid product) وهذا يدل على أنه
+
+296
+00:21:21,290 --> 00:21:24,690
+صار هناك تخمير مختلط للأحماض (mixed acid fermentation).
+
+297
+00:21:24,690 --> 00:21:29,810
+سنلاحظ أن اللون سيُحوّل إلى أحمر بعد أن أضفتُ
+
+298
+00:21:29,810 --> 00:21:33,130
+الكاشف (reaction) للميثيل ريد (Methyl Red)،  فالبكتيريا كانت E.
+
+299
+00:21:33,130 --> 00:21:37,250
+coli الموجبة (positive) لهذا الفحص (Methyl Red).  E.coli
+
+300
+00:21:37,250 --> 00:21:40,610
+Antirobacter قلنا سالبة (negative)، لا يحدث أي تغير في
+
+301
+00:21:40,610 --> 00:21:44,020
+لون الوسط (media)؛ إذا لاحظتم، غير مُحضّن (unincubation)  لم تُزرع.
+
+302
+00:21:44,020 --> 00:21:47,800
+لم تُزرع؛ يكون لون الوسط (media)  لا يوجد أي تغير في لون
+
+303
+00:21:47,800 --> 00:21:50,980
+الوسط (media) ،فهي سالبة (negative) مثل الإيكلسيلا (Eclipsilla) و ال
+
+304
+00:21:50,980 --> 00:21:55,320
+Enterobacter. فحص فوجس-بروكساور (Voges-Proskauer Test)  وللبكتيريا الشكلية (coliform) الأخرى
+
+305
+00:21:55,320 --> 00:22:00,740
+التي لم تُخمر (ferment) الأحماض المختلطة (mix acid)، بالتأكيد ستُخمر (ferment) بفحص فوجس-بروكساور (Voges-Proskauer Test)  أو
+
+306
+00:22:00,740 --> 00:22:05,580
+مسار البيوتانديول (butanediol pathway)؛ تحوّل الدكستروز (dextrose)  الجلوكوز
+
+307
+00:22:05,580 --> 00:22:11,620
+إلى منتجات أقل حمضية (to less acidic product)، منتجات كحولية
+
+308
+00:22:11,620 --> 00:22:16,160
+(product) مثل الإيثانول (ethanol) أو البيوتانديول (butanediol). هذه البكتيريا
+
+309
+00:22:16,160 --> 00:22:20,780
+أقول لكم، ستكون سالبة (negative) في فحص الميثيل ريد (methyl red) لو قمتُ
+
+310
+00:22:20,780 --> 00:22:24,040
+بفحص الميثيل ريد (methyl red)، ستكون سالبة (negative) في الميثيل ريد (methyl red)  لكن
+
+311
+00:22:24,040 --> 00:22:27,220
+موجبة (positive) في فوجس-بروكساور (Voges-Proskauer).  تخمير البيوتانديول (Butanediol fermentation)
+
+312
+00:22:27,220 --> 00:22:31,300
+في مسار (pathway) هذا قلنا يجب أن أتمكن من الكشف عنه بواسطة
+
+313
+00:22:31,300 --> 00:22:35,560
+فحص فوجس-بروكساور (Voges-Proskauer Test)؛ يكشف عن وجود الأسيتون (Acetone)؛ لأنه
+
+314
+00:22:35,560 --> 00:22:39,920
+قلنا الكاشف VB1 يرتبط مع الأسيتون (Acetone) وليس
+
+315
+00:22:39,920 --> 00:22:43,660
+مع البيوتانديول (Butanediol). فهو سيكشف عن وجود الأسيتون (Acetone)
+
+316
+00:22:43,660 --> 00:22:47,240
+الذي يكون أساسياً في تكوين البيوتانديول (Butanediol).  البيوتانديول (Butanediol)
+
+317
+00:22:47,240 --> 00:22:51,980
+الأسيتون (Acetone) يتحول إلى بيوتانديول (Butanediol).  البيوتانديول (Butanediol) هو
+
+318
+00:22:51,980 --> 00:22:55,960
+مستقر (stable) ومتعادل (neutral)
+
+319
+00:22:55,960 --> 00:22:58,120
+مستقر (stable) ومتعادل (neutral).
+
+320
+00:23:00,050 --> 00:23:04,910
+في هذين الفحصين (methyl red test and Voges-Proskauer)
+
+321
+00:23:04,910 --> 00:23:07,690
+test. نستخدم نفس الوسط (media) لأن
+
+322
+00:23:07,690 --> 00:23:11,430
+الركيزة (substrate) نفسها هي الجلوكوز (glucose)، لكن الكاشف (reagent) يختلف
+
+323
+00:23:11,430 --> 00:23:17,700
+أضيف بعد الحضانة (incubation). أقول لكم أنني أقوم بهذين الفحصين
+
+324
+00:23:17,700 --> 00:23:24,740
+في نفس الوقت لأرى، لأرى يعني، إذا كانت
+
+325
+00:23:24,740 --> 00:23:28,640
+موجبة (positive) لأحدهما، ستكون سالبة (negative) للأخرى لكي
+
+326
+00:23:28,640 --> 00:23:32,480
+أتأكد من أن إحداهما موجبة (positive) والأخرى يجب أن تكون
+
+327
+00:23:32,480 --> 00:23:37,460
+سالبة (negative).  كاشف فوجس-بروكساور (Voges-Proskauer reagent)  كاشف فوجس-بروكساور (Voges-Proskauer reagent) للكشف
+
+328
+00:23:37,460 --> 00:23:42,920
+عن فحص فوجس-بروكساور (Voges-Proskauer test). قلنا VB1  5% α-نفثول،
+
+329
+00:23:42,920 --> 00:23:47,460
+VB2 وهو 40% هيدروكسيد البوتاسيوم (potassium hydroxide) KOH. أضيفه
+
+330
+00:23:47,460 --> 00:23:54,480
+بعد 24 ساعة (to 24-hour culture). قلنا بعد أن تكون
+
+331
+00:23:54,480 --> 00:23:59,080
+المزرعة قد خضعت للحضانة (incubation) 24 ساعة، ممنوع أن أضعه
+
+332
+00:23:59,080 --> 00:24:03,900
+بعد 72 ساعة. الأنبوب يُرج جيداً، أحاول أن
+
+333
+00:24:03,900 --> 00:24:09,890
+أرج جيداً ليمتزج، يتحول اللون إلى وردي (pink)
+
+334
+00:24:09,890 --> 00:24:14,770
+أو أحمر (red) بعد أن أقوم بالرج (shaking). هذا يدل على
+
+335
+00:24:14,770 --> 00:24:18,430
+نتيجة موجبة (positive reaction) لوجود الأسيتون (Acetone)
+
+336
+00:24:18,430 --> 00:24:22,410
+وليس البيوتانديول (Butanediol)؛ لأنه قلنا الكاشف
+
+337
+00:24:22,410 --> 00:24:25,850
+يرتبط مع الأسيتون (Acetone) وليس البيوتانديول (Butanediol) لذلك
+
+338
+00:24:25,850 --> 00:24:32,490
+يجب أن لا تتجاوز مدة الحضانة (incubation) للمزرعة 72 ساعة. نلاحظ في
+
+339
+00:24:32,490 --> 00:24:37,100
+فحص فوجس-بروكساور (Voges-Proskauer)، سيكون لون الوسط (media) أصفر (yellow). هذه ليست
+
+340
+00:24:37,100 --> 00:24:40,860
+مُحضّنة (unincubation)، هذه هي نفسها  للميثيل ريد (Methyl red) الموجبة (Positive)،
+
+341
+00:24:40,860 --> 00:24:43,860
+سنلاحظ وجود لون أحمر (red). قلنا الموجبة (positive) في
+
+342
+00:24:43,860 --> 00:24:46,420
+فحص فوجس-بروكساور (Voges-Proskauer) هي الإنتروباكتر (Enterobacter) و ال
+
+343
+00:24:46,420 --> 00:24:49,000
+الإيكلسيلا (Eclipsilla) التي كانت سالبة (Negative) في الميثيل ريد (Methyl red)
+
+344
+00:24:49,000 --> 00:24:51,120
+هذه الإيكلسيلا الرئوية (Eclipsilla pneumonia) والانتروباكتر
+
+345
+00:24:51,120 --> 00:24:54,970
+موجبة (Positive) في فحص فوجس-بروكساور (Voges-Proskauer)، لكن E.coli و
+
+346
+00:24:54,970 --> 00:24:58,690
+البروتيوس (Proteus) قلنا ستكون سالبة (negative) في فحص فوجس-بروكساور (Voges-Proskauer)
+
+347
+00:24:58,690 --> 00:25:03,070
+لم يتغير لون الوسط (media) لديهم لأنهم موجبة (positive) في
+
+348
+00:25:03,070 --> 00:25:06,210
+الميثيل ريد (methyl red)؛ لأنهم يُخمرون (ferment) الأحماض المختلطة (mixed acid fermentation).
+
+349
+00:25:06,210 --> 00:25:10,730
+في الواقع، هناك بعض الملاحظات التي ذكرناها أيضاً. لو
+
+350
+00:25:10,730 --> 00:25:14,530
+البكتيريا لا تُخمر (ferment) أصلاً الجلوكوز (glucose)  لو
+
+351
+00:25:14,530 --> 00:25:17,790
+قمتُ بهذا الفحص (methyl red and Voges-Proskauer test)
+
+352
+00:25:17,790 --> 00:25:22,930
+ستكون سالبة (negative) في كليهما مثل السيودوموناس (Pseudomonas). أقول لكم، من الضروري
+
+353
+00:25:22,930 --> 00:25:28,190
+أن تضعوا الكاشف (reagent)  بشكل مُتسلسل. لو أضفتم KOH
+
+354
+00:25:28,190 --> 00:25:31,590
+وهو VB2 أولاً، سيتفاعل مع الببتون
+
+355
+00:25:31,590 --> 00:25:36,330
+الموجود في الوسط (media)، ويعطيني لون وردي سلموني (Salmon pink color) وهو
+
+356
+00:25:36,330 --> 00:25:40,390
+لون السلمون، وبالتالي سأظنه هو
+
+357
+00:25:40,390 --> 00:25:43,830
+النتيجة الموجبة (positive result)، لأن النتيجة الموجبة (positive result) تكون
+
+358
+00:25:43,830 --> 00:25:48,790
+عند لون أحمر، لكن هذا نتيجة موجبة خاطئة (false positive result).  KOH
+
+359
+00:25:48,790 --> 00:25:53,350
+الذي يرتبط مع الببتون (peptone)، لكن قلنا الكاشف
+
+360
+00:25:53,350 --> 00:25:57,470
+VB1 يرتبط مع الأسيتون (acetylene) ليعطيني اللون
+
+361
+00:25:57,470 --> 00:26:01,470
+الأحمر.  فحص فوجس-بروكساور (Voges-Proskauer test). قلنا فحص فوجس-بروكساور (Voges-Proskauer) يجب أن تكون
+
+362
+00:26:01,470 --> 00:26:05,450
+المزرعة، ممنوع أن أقوم به بعد 72 ساعة من
+
+363
+00:26:05,450 --> 00:26:11,630
+الحضانة (incubation)، لأنه سيُعطيني نتيجة موجبة خاطئة (weekly positive or false)
+
+364
+00:26:11,630 --> 00:26:16,450
+أو سالبة خاطئة (negative result). لماذا؟ لأننا قلنا إنه بعد 72 ساعة
+
+365
+00:26:16,450 --> 00:26:20,430
+الأسيتون (Acetone) غير المستقر (Unstable) يتحول إلى مركب
+
+366
+00:26:20,430 --> 00:26:24,150
+البيوتانديول (butanediol) المستقر (stable). وقلنا أن الكاشف V.1
+
+367
+00:26:24,150 --> 00:26:28,110
+يرتبط مع الأسيتون (Acetone) وليس مع البيوتانديول (butanediol). فلو
+
+368
+00:26:28,110 --> 00:26:33,130
+وضعته بعد 72 ساعة، سيكون قد تحول إلى بيوتانديول (butanediol)، سيبحث
+
+369
+00:26:33,130 --> 00:26:36,370
+عن الأسيتون (Acetone)، لن يجده، وبالتالي لن يرتبط
+
+370
+00:26:36,370 --> 00:26:40,870
+معه، لن يُعطيني اللون الأحمر (Red)، فسأظن النتيجة
+
+371
+00:26:40,870 --> 00:26:44,640
+سالبة (Negative)، لكنها سالبة خاطئة (False Negative Result) لأنني
+
+372
+00:26:44,640 --> 00:26:48,900
+استخدمتُ مزرعة بعد 72 ساعة من الحضانة (incubation)، حيث
+
+373
+00:26:48,900 --> 00:26:52,940
+نكون قد أنهينا فحص الميثيل ريد (Methyl Red) وفوجس-بروكساور (Voges-Proskauer Test)
+
+374
+00:26:52,940 --> 00:26:5
\ No newline at end of file
diff --git a/PL9fwy3NUQKwZWgAb_V1ATlRRsH-6i3cPC/fiNRLFNYLh8_raw.srt b/PL9fwy3NUQKwZWgAb_V1ATlRRsH-6i3cPC/fiNRLFNYLh8_raw.srt
new file mode 100644
index 0000000000000000000000000000000000000000..2276c80cd39e0c05517b496f099c4d6fd352d250
--- /dev/null
+++ b/PL9fwy3NUQKwZWgAb_V1ATlRRsH-6i3cPC/fiNRLFNYLh8_raw.srt
@@ -0,0 +1,1656 @@
+1
+00:00:02,330 --> 00:00:06,610
+Methyl redvocas-buscowar test هذا الفحص له علاقة
+
+2
+00:00:06,610 --> 00:00:09,990
+بالبكتيريا ال fermentation لو البكتيريا عملت
+
+3
+00:00:09,990 --> 00:00:14,710
+fermentation للجليكوز هعمل فحص الـ Methyl redvocas
+
+4
+00:00:14,710 --> 00:00:18,950
+-buscowar قولنا إن من نتائج عملية ال fermentation
+
+5
+00:00:18,950 --> 00:00:24,210
+ال end product إما يكون أسد أو الكحول وقلنا إن ال
+
+6
+00:00:24,210 --> 00:00:27,210
+end product هي من خلالها أنا بقدر أعمل
+
+7
+00:00:27,210 --> 00:00:30,570
+identification للبكتيرياكيف بدي اعمل
+
+8
+00:00:30,570 --> 00:00:33,890
+identification للبكتيريا من خلال الـ end product
+
+9
+00:00:33,890 --> 00:00:38,470
+من خلال فحص الـ methyl red vocas buscala عشان نعرف
+
+10
+00:00:38,470 --> 00:00:49,090
+أكتر لو
+
+11
+00:00:49,090 --> 00:00:52,810
+البكتيريا عملت fermentation للجليكوز عملت فحص
+
+12
+00:00:52,810 --> 00:00:57,070
+البكتيريا ال fermentation اللي شرحناه في الجزء
+
+13
+00:00:57,070 --> 00:01:01,580
+الأولبقول لي بتعمل فحص الـ Methyl Red Vagas
+
+14
+00:01:01,580 --> 00:01:05,160
+Buscowar Test الـ Methyl Red Vagas Buscowar Test
+
+15
+00:01:05,160 --> 00:01:09,980
+هذا من خلاله بدي أعرف أي pathway سلكت البكتيريا
+
+16
+00:01:09,980 --> 00:01:15,580
+البكتيريا لما تنتج acid بقول لي بتسلق ال pathway
+
+17
+00:01:15,580 --> 00:01:19,600
+اسمه mixed acid fermentation يبقى لو كان ناتج
+
+18
+00:01:19,600 --> 00:01:24,480
+عملية ال fermentation acid بتكون البكتيريا سلكت ال
+
+19
+00:01:24,480 --> 00:01:28,870
+pathway اللي اسمه mixed acid fermentationلو كان
+
+20
+00:01:28,870 --> 00:01:33,070
+ناتج عملية ال fermentation ألكحول بتكون البكتيريا
+
+21
+00:01:33,070 --> 00:01:38,470
+سلكت ال pathway اللي اسمه butylene glycol pathway
+
+22
+00:01:38,470 --> 00:01:44,750
+or neutral pathway or butanediol pathway تلت أسامي
+
+23
+00:01:44,750 --> 00:01:51,510
+ل ال pathway هذا اللي نواتج ال fermentation بتكون
+
+24
+00:01:51,510 --> 00:01:56,480
+ألكحول بالنسبة ل ال mixed acid fermentationأنا
+
+25
+00:01:56,480 --> 00:02:01,020
+عشان أكشف هل البكتيريا سلكت هذا ال pathway بستخدم
+
+26
+00:02:01,020 --> 00:02:06,560
+فحص الـ Methyl Red Test عشان أعرف البكتيريا سلكت
+
+27
+00:02:06,560 --> 00:02:10,940
+ال pathway اللي اسمه neutral pathway او بيوتانديول
+
+28
+00:02:10,940 --> 00:02:16,580
+او بيوتيولين ايش اللي يقول pathway بستخدم فحص ال
+
+29
+00:02:16,580 --> 00:02:20,640
+AUGUST BUSCUAR TEST بالنسبة لـMixed Acid
+
+30
+00:02:20,640 --> 00:02:23,980
+Fermentation قلنا ان البكتيريا بتعمل ferment
+
+31
+00:02:23,980 --> 00:02:29,280
+للجليكوزand produce extreme amount of acid قولنا
+
+32
+00:02:29,280 --> 00:02:32,720
+نواتج العملية في هذا ال pathway acid بقول extreme
+
+33
+00:02:32,720 --> 00:02:37,460
+amount of acid بتكون ال acid strong acid زي ال
+
+34
+00:02:37,460 --> 00:02:41,160
+formic acid الacidic acid و ال lactic acid هاي ال
+
+35
+00:02:41,160 --> 00:02:46,060
+acid تعتبر strong acid فبالتالي هتعمل على خفط ال
+
+36
+00:02:46,060 --> 00:02:52,140
+pH بشكل كبير high decrease in pH أقل من خمسةالـ pH
+
+37
+00:02:52,140 --> 00:02:57,500
+بوصل فبالتالي بيعمل على تغيير لون ال media في
+
+38
+00:02:57,500 --> 00:03:02,040
+الفحص ال bacterial fermentation لو عملته بشكل بشكل
+
+39
+00:03:02,040 --> 00:03:06,380
+كبير بيعمل على تغيير ال pH بيخفض ال pH بشكل كبير
+
+40
+00:03:06,380 --> 00:03:10,160
+عشان هيك بفحص ال bacterial fermentation اللي حكينا
+
+41
+00:03:10,160 --> 00:03:16,320
+عنهكان في لتيوب لون اللي لتيوب كان أصفر غامق أصفر
+
+42
+00:03:16,320 --> 00:03:20,380
+غامق قلنا لأنه بتنتج كمية كبيرة من ال acid strong
+
+43
+00:03:20,380 --> 00:03:24,780
+acid يبقى كانت في لتيوب هاي ال pathway mixed acid
+
+44
+00:03:24,780 --> 00:03:29,320
+pathway كان extreme amount of acid بقولي نواتج
+
+45
+00:03:29,320 --> 00:03:33,000
+عملية ال fermentation في ال mixed acid
+
+46
+00:03:33,000 --> 00:03:38,300
+fermentation بتكون stable عشان أكشف عن هاي ال
+
+47
+00:03:38,300 --> 00:03:42,720
+pathwayبضيف reagent اسمه missile red reagent
+
+48
+00:03:42,720 --> 00:03:45,780
+هنتعرف على ال procedure ومتى انا هضيفها ده ال
+
+49
+00:03:45,780 --> 00:03:51,380
+missile red بالنسبالى ال neutral pathway او
+
+50
+00:03:51,380 --> 00:03:55,080
+butanediol pathway او butylene glycol pathway
+
+51
+00:03:55,080 --> 00:03:58,820
+بقوللي ان البكتيريا بتعمل fermentation للجليكوز
+
+52
+00:03:58,820 --> 00:04:04,840
+لكن بتنتج الكحول اللي هو الacetoneالاسيتون يعتبر
+
+53
+00:04:04,840 --> 00:04:09,180
+الكحول من نواتج عملية ال fermentation هذا الاسيتون
+
+54
+00:04:09,180 --> 00:04:14,060
+يعتبر unstable على عكس ال strong acid كانت هنا في
+
+55
+00:04:14,060 --> 00:04:18,060
+ال mixed acid pathway stable لكن الاسيتون يعتبر
+
+56
+00:04:18,060 --> 00:04:23,200
+unstable acidic product او هو يعتبر acidic لكن مش
+
+57
+00:04:23,200 --> 00:04:27,380
+strong acid هيعمل على خفض ال pH لكن مش بصورة كبيرة
+
+58
+00:04:27,380 --> 00:04:32,460
+هيكون slight decrease in pHبقول لي بما ان هو
+
+59
+00:04:32,460 --> 00:04:38,620
+unstable اكيد عشان يصير مستقر هيتحول لمركب اكثر
+
+60
+00:04:38,620 --> 00:04:43,920
+استقرارا هيتحول لبيوتان ديول هذا البيوتان ديول
+
+61
+00:04:43,920 --> 00:04:48,640
+stable عشان هيك سميت ال pathway اسمه بيوتان ديول
+
+62
+00:04:48,640 --> 00:04:52,960
+pathwayهذا المُركّب بقول لي neutral product عشان
+
+63
+00:04:52,960 --> 00:04:55,900
+هيك سميت ال pathway اسم تاني كان ال neutral
+
+64
+00:04:55,900 --> 00:04:59,880
+pathway يبقى هيعمللي slight decrease in pH عشان
+
+65
+00:04:59,880 --> 00:05:04,960
+هيك ال tube كانت في الجزء القبل في المحاضرة لما
+
+66
+00:05:04,960 --> 00:05:09,220
+كشفت عن ال fermentation كان اللون فاتح اتكون
+
+67
+00:05:09,220 --> 00:05:13,100
+القحول عمللي على خفض ال pH لكن كان slight decrease
+
+68
+00:05:13,100 --> 00:05:19,910
+in pHعشان اوضح الصورة اكتر او ان انا اعرف هل هي
+
+69
+00:05:19,910 --> 00:05:23,990
+سلكة الـVB pathway اللي هو الـbutanediol pathway
+
+70
+00:05:23,990 --> 00:05:27,510
+او الـmixed acid pathway لازم اعمل فحص
+
+71
+00:05:27,510 --> 00:05:30,930
+الـmethyredvo-casposcavar لان اوقات ممكن مايكون في
+
+72
+00:05:30,930 --> 00:05:34,930
+عندي الـtube ان انا اقارن الفرق في اللون هل هو
+
+73
+00:05:34,930 --> 00:05:38,110
+غامق او فاتح ممكن تطلع كلها البكتيريا اللي عندي
+
+74
+00:05:38,110 --> 00:05:41,910
+سلكة طريقة الـmixed acid pathwayفبالتالي مش هقدر
+
+75
+00:05:41,910 --> 00:05:45,310
+اني انا افرد فعشان هيك عملوا فحص الـ Methyl Red
+
+76
+00:05:45,310 --> 00:05:49,550
+Vukasposkaur الـ Reagent اللي انا بدي اضيفه عشان
+
+77
+00:05:49,550 --> 00:05:53,230
+اكشف على هذا ال pathway اسمه Barrett's Reagent الـ
+
+78
+00:05:53,230 --> 00:05:57,870
+Barrett's Reagent بتكون من VB1 VB نتيجة اختصار ل
+
+79
+00:05:57,870 --> 00:06:02,270
+ال V اسم الفحص اللي هو Vukasposkaurالأول الـ
+
+80
+00:06:02,270 --> 00:06:05,070
+reagent اللى أنا بضيفه اللى هو خمسة في المية Alpha
+
+81
+00:06:05,070 --> 00:06:10,310
+-Naphthol VB2 اللى بدي أضيفه بعد ما ضفت الأول هضيف
+
+82
+00:06:10,310 --> 00:06:17,770
+reagent أربعين في المية KOH KOH هو هذا ال VB2 يبقى
+
+83
+00:06:17,770 --> 00:06:22,110
+البرتز reagent بتكون من VB1 و VB2 ال VB1 Alpha
+
+84
+00:06:22,110 --> 00:06:28,110
+-Naphthol ال VB2 أربعين في المية KOH طيب كيف أنا
+
+85
+00:06:28,110 --> 00:06:34,330
+بديأكشف على ال path way أي path way سلكة البكتيريا
+
+86
+00:06:34,330 --> 00:06:40,110
+بقوللي اني بدي اجيب media اسمها methyl red vucus
+
+87
+00:06:40,110 --> 00:06:43,090
+puscawar هاي ال media بتكون ال broth الميديا
+
+88
+00:06:43,090 --> 00:06:48,390
+بتحتوي على جيليكوز اكيد ك substrate هيشتغل عليها
+
+89
+00:06:48,390 --> 00:06:52,790
+هتشتغل عليها البكتيريا هتعمل fermentation لجيليكوز
+
+90
+00:06:52,790 --> 00:06:58,410
+في عندى في الميديا برضه ببطونمصدر للـ nitrogen
+
+91
+00:06:58,410 --> 00:07:02,550
+source عشان تنمو البكتيريا بولي هاي ال media
+
+92
+00:07:02,550 --> 00:07:07,470
+بتاخدي two tube منها واحد ال tube هيكون لفحص ال
+
+93
+00:07:07,470 --> 00:07:11,250
+methyl red test وال tube التاني هيكون لفحص ال VB
+
+94
+00:07:11,250 --> 00:07:17,980
+testنفس السبسترات لأن نفس السبسترات هيشتغلوا عليها
+
+95
+00:07:17,980 --> 00:07:22,420
+فبالتالي هتكون نفس ال media في كيلاء الفحصين لكن
+
+96
+00:07:22,420 --> 00:07:26,040
+الاختيلة في ال reagent اللي انا هضيفه بعد ال
+
+97
+00:07:26,040 --> 00:07:29,380
+incubation بعد ال incubation لفحص ال methyl red
+
+98
+00:07:29,380 --> 00:07:32,580
+هضيف ال reagent اللي هو قولنا methyl red reagent
+
+99
+00:07:32,580 --> 00:07:36,540
+لكن لفحص ال focus بسكوت test هضيف ال barretts
+
+100
+00:07:36,540 --> 00:07:41,820
+reagent يبقى انا بستخدمهدول الفحصين باستخدام لهم
+
+101
+00:07:41,820 --> 00:07:45,120
+نفس ال media هاي ال media بتحتوي على جليكوز ك
+
+102
+00:07:45,120 --> 00:07:49,120
+substrate بقول لي نفس ال organism بدي أزرعه في
+
+103
+00:07:49,120 --> 00:07:53,680
+توقت يوب هعمل incubation لمدة 24 ساعة على درجة
+
+104
+00:07:53,680 --> 00:07:57,760
+حرارة 37 بعد ما تخلص فترة ال incubation و تم
+
+105
+00:07:57,760 --> 00:08:02,820
+البكتيريا أكيد هتكون عاملة fermentation لجليكوز
+
+106
+00:08:02,820 --> 00:08:06,420
+تمام أنا بالشكل المبدأي عرفت أنه هي جليكوز
+
+107
+00:08:06,420 --> 00:08:10,100
+fermenter من الفحص الليحكينا عنه في الجزء الاول
+
+108
+00:08:10,100 --> 00:08:13,540
+هلا بدي اعرف اي pathway سلكة ال mixed acid pathway
+
+109
+00:08:13,540 --> 00:08:16,000
+او بيوتان بيوتان بيوتان بيوتان بيوتان بيوتان
+
+110
+00:08:16,000 --> 00:08:17,280
+بيوتان بيوتان بيوتان بيوتان بيوتان بيوتان بيوتان
+
+111
+00:08:17,280 --> 00:08:24,760
+بيوتان بيوتان بيوتان بيوتان بيوتان بيوتان بيوتان
+
+112
+00:08:24,760 --> 00:08:27,780
+بيوتان بيوتان بيوتان بيوتان بيوتان بيوتان بيوتان
+
+113
+00:08:27,780 --> 00:08:27,780
+بيوتان بيوتان بيوتان بيوتان بيوتان بيوتان بيوتان
+
+114
+00:08:27,780 --> 00:08:27,780
+بيوتان بيوتان بيوتان بيوتان بيوتان بيوتان بيوتان
+
+115
+00:08:27,780 --> 00:08:32,120
+بيوتان بيوتان بيوتان بيوتان بيوتان بيوتان بيوتان
+
+116
+00:08:32,120 --> 00:08:37,590
+بهيعمل على خفض ال pH بصورة كبيرة high decrease in
+
+117
+00:08:37,590 --> 00:08:42,590
+pH هضيف اكيد pH indicator بقول ال pH indicator في
+
+118
+00:08:42,590 --> 00:08:47,990
+هذا الفحص هو ال methyl red مش بيكون موجود في ال
+
+119
+00:08:47,990 --> 00:08:51,430
+media انا بضيفه بعد ما اتخلص incubation بعد ما
+
+120
+00:08:51,430 --> 00:08:54,570
+اتخلص فترة ال incubation بضيف ال pH indicator
+
+121
+00:08:54,570 --> 00:08:59,270
+بيكون ال reagent لونه أحمر بضيف two drop من one ل
+
+122
+00:08:59,270 --> 00:09:05,210
+three drop على ال tubeالميثايرد رياجنت شو حدوده
+
+123
+00:09:05,210 --> 00:09:09,610
+بيقول لي بيكون في ال acidic لونه أحمر وفي ال basic
+
+124
+00:09:09,610 --> 00:09:14,170
+بيكون لونه أصفر لنيوترال بيكون ما بين 4 و 4 و 6 و
+
+125
+00:09:14,170 --> 00:09:18,570
+2 لونه orange ننتبه بالفحص اللي اتكلمنا عنه بالأول
+
+126
+00:09:18,570 --> 00:09:21,090
+ال fermentation عشان أشوف بشكل عام هل هي بتعمل
+
+127
+00:09:21,090 --> 00:09:26,510
+fermentation ولا لأ كانالـ pH Indicator في نول رد
+
+128
+00:09:26,510 --> 00:09:28,770
+وكان موجود في ال media أنا ماضفته بعد ال
+
+129
+00:09:28,770 --> 00:09:32,670
+incubation الحدود معاكوسة كانت هناك في ال acidic
+
+130
+00:09:32,670 --> 00:09:37,130
+yellow وفي ال basic لون red لكن هنا في ال acidic
+
+131
+00:09:37,130 --> 00:09:42,230
+لونه أحمر وفي ال basic لون yellow يبقى اتكون عندي
+
+132
+00:09:42,230 --> 00:09:46,710
+من نتيجة ال fermentation strong acid هتعمل على خفض
+
+133
+00:09:46,710 --> 00:09:53,960
+ال pH بصورة كبيرة هتكون أقل من 4.4هيتحول اللون
+
+134
+00:09:53,960 --> 00:09:57,120
+اللون للون red يبقى النتيجة ال positive هان red
+
+135
+00:09:57,120 --> 00:10:00,180
+كانت هناك في ال fermentation بالبكتيريا ال
+
+136
+00:10:00,180 --> 00:10:03,920
+fermentation كانت النتيجة ال yellow كانت هي ال
+
+137
+00:10:03,920 --> 00:10:09,180
+positive لأن ال indicator بيختلف لون ال media
+
+138
+00:10:09,180 --> 00:10:13,700
+لونها أصفر اللي هنزرع عليها فلو ما تغير أي شيء ضال
+
+139
+00:10:13,700 --> 00:10:16,760
+لون ال media لونه yellow يبقى هي negative من
+
+140
+00:10:16,760 --> 00:10:20,620
+الأمثلة على ال positive ال E.coli و ال Brutias
+
+141
+00:10:20,620 --> 00:10:24,350
+mirabilisمن الأمثلة على الـ Negative
+
+142
+00:10:24,350 --> 00:10:27,970
+الكليبسيلانيومانيا و الـ Enterobacter يبقى هذا
+
+143
+00:10:27,970 --> 00:10:32,390
+الفحص الـ Methyl Red Vox Biscuit يستخدم لتفريق
+
+144
+00:10:32,390 --> 00:10:36,010
+الكوليفارم بكتيريا قلنا الكوليفارم بكتيريا اللي هي
+
+145
+00:10:36,010 --> 00:10:40,110
+الـ E.coli الستروبكتر الـ Enterobacter و
+
+146
+00:10:40,110 --> 00:10:43,350
+الكليبسيلة عشان أفرق بينهم أخدنا فحص السيطرات
+
+147
+00:10:43,350 --> 00:10:46,770
+بقولي برضه بتستخدمي فحص الـ Methyl Red Vox Biscuit
+
+148
+00:10:46,770 --> 00:10:51,350
+و هنستخدم برضه فحص الاندول هذا فيما يسمى بـ MVEC
+
+149
+00:10:51,660 --> 00:10:55,020
+الـ M-Vecto استخدم لتمييز الكوليفورم بش تغير
+
+150
+00:10:55,020 --> 00:10:58,420
+الأربع فحوصات عشان أفرق الكوليفورم بكتيريا
+
+151
+00:10:58,420 --> 00:11:01,240
+الإكليبسيلا السيتروباكتر الانتيروباكتر وال E-coli
+
+152
+00:11:01,240 --> 00:11:05,760
+هذا بالنسبة لـ Methyl Red بالنسبة للـ Vocus
+
+153
+00:11:05,760 --> 00:11:08,840
+Biscuit Test بعد ما أنا أعمل incubation و أتخلص
+
+154
+00:11:08,840 --> 00:11:12,520
+فترة ال incubation عشان أكشف عنه بضيف البرتز
+
+155
+00:11:12,520 --> 00:11:15,900
+رياجون نفس الفكرة هضيف البرتز رياجون بعد ال
+
+156
+00:11:15,900 --> 00:11:21,480
+incubation وقوللي بتضيفي VB1 بعدين بتضيفي VB2ممنوع
+
+157
+00:11:21,480 --> 00:11:27,160
+أنا أبدل هنحكي السبب بعد ما نشرح كيف أو إيهش
+
+158
+00:11:27,160 --> 00:11:32,780
+التفاعل اللي بيصير قولنا من نواتج عملية ال ال
+
+159
+00:11:32,780 --> 00:11:36,400
+pathway اللي هو الـ butanediol pathway أسيتوين
+
+160
+00:11:36,400 --> 00:11:40,760
+الأسيتوين هو ألكحول قولنا ألكحول الأمثلة عليها
+
+161
+00:11:40,760 --> 00:11:45,520
+الأسيتوين الأسيتوين يعتبر acidic لكن ما بيعمل على
+
+162
+00:11:45,520 --> 00:11:51,000
+خفض الـ pH بصورة كبيرة UnstableReagent VB1 اللي هو
+
+163
+00:11:51,000 --> 00:11:55,000
+الـ Alpha-Naphthol برتبط في الـ Acetone وبعطيني
+
+164
+00:11:55,000 --> 00:11:59,280
+Complex بعدها أنا هضيف بعدى بمدة تقريبا 10 دقايق
+
+165
+00:11:59,280 --> 00:12:06,240
+هضيف الـ VB2 الـ VB2 اللي هو الـ Hydrogen الـ KOH
+
+166
+00:12:06,240 --> 00:12:10,940
+الـ KOH هيرتبط بهذا الكمplex ويعطينى اللون الـ Red
+
+167
+00:12:10,940 --> 00:12:15,580
+يبقى النتيجة الـ Positive هتكون Red لكن النتيجة
+
+168
+00:12:15,580 --> 00:12:18,000
+الـ Negative مش هيصير تغير في الميديا لون الميديا
+
+169
+00:12:18,000 --> 00:12:22,560
+لونها أصفرالبكتيريا الـ positive لهذا الفحص
+
+170
+00:12:22,560 --> 00:12:25,480
+كليبسيلا نيومانيا و Enterobacter لكن الـ negative
+
+171
+00:12:25,480 --> 00:12:29,520
+الـ E.coli و البروتياس ميرابيلاس نلاحظ أن اللي
+
+172
+00:12:29,520 --> 00:12:33,580
+كانت positive في الـ methyl red هتكون negative في
+
+173
+00:12:33,580 --> 00:12:36,180
+الـ Vagus Buscal و اللي كانت negative في الـ
+
+174
+00:12:36,180 --> 00:12:40,800
+methyl red هتكون positive في الـ Vagus Buscal يبقى
+
+175
+00:12:40,800 --> 00:12:44,800
+هذا الفحص أكيد البكتيريا اللي سلكت الـ mixed acid
+
+176
+00:12:44,800 --> 00:12:49,560
+pathwayمش هتسلق الطريق التاني إما هتسلق طريق الـ
+
+177
+00:12:49,560 --> 00:12:53,040
+mixed acid pathway أو طريق اللي هو الـ butanediol
+
+178
+00:12:53,040 --> 00:12:57,380
+pathway مش هتسلق اتينين مع بعض يبقى بعمل الفحص الـ
+
+179
+00:12:57,380 --> 00:13:00,660
+methyl red لبكتيريا الـ E.coli إش طبيعي يكون الـ
+
+180
+00:13:00,660 --> 00:13:03,880
+vascular اللي لها ال E.coli بما أنها كانت positive
+
+181
+00:13:03,880 --> 00:13:06,500
+في ال methyl red إش طبيعي هيكون negative في ال E
+
+182
+00:13:06,500 --> 00:13:12,780
+.coli لأنها تسلق فقط pathway واحد لكنلو طلعت نيجة
+
+183
+00:13:12,780 --> 00:13:16,400
+في الـ Methyl Red فأكيد هتكون سالكة الطريق التاني
+
+184
+00:13:16,400 --> 00:13:18,900
+اللي هو الـ butanediol فهتكون positive في الـ
+
+185
+00:13:18,900 --> 00:13:24,460
+focus بوسكوية هذا الفحص كيف يشتغل الفحص و كيف
+
+186
+00:13:24,460 --> 00:13:28,380
+نشتغل الفحص و الـ principle لهذا الفحص أنه في عندي
+
+187
+00:13:28,380 --> 00:13:33,800
+Methyl Red بيعمل drop في الـ mixed acid pathway
+
+188
+00:13:33,800 --> 00:13:37,520
+فيه بيكون عندي high decrease in pH فبالتالي هيتحول
+
+189
+00:13:37,520 --> 00:13:43,080
+لون الـ pH indicator للون أحمريلكن هنا مافي NDPH
+
+190
+00:13:43,080 --> 00:13:47,640
+Indicator هضيف Reagent اللي هو الـ VB1 و VB2 الـ
+
+191
+00:13:47,640 --> 00:13:52,020
+VB1 برتبط مع الـ Acetone انتباه وليس البيوتانديون
+
+192
+00:13:52,020 --> 00:13:55,660
+مع الـ Acetone الـ Unstable هيعطيني بالنهاية
+
+193
+00:13:55,660 --> 00:13:58,540
+Complex هذا الـ Complex هيرتبط بالـ VB2 هيعطيني
+
+194
+00:13:58,540 --> 00:14:01,900
+اللون الـ Red يبقى اللون الـ Red نتج مش نتيجة انه
+
+195
+00:14:01,900 --> 00:14:05,600
+كان في NDPH Indicator نتيجة Reagent اتفاعل مع
+
+196
+00:14:05,600 --> 00:14:11,000
+نواتج التفاعليبقى ارتبط مع نواتج التفاعل الـ
+
+197
+00:14:11,000 --> 00:14:14,380
+Acetyl-Nucleotanyl-1 مش نتيجة الـ pH Integrator أو
+
+198
+00:14:14,380 --> 00:14:20,740
+تغيير في الـ pH فيها نقطتين مهمين لازم نتبهلقلهم
+
+199
+00:14:20,740 --> 00:14:26,640
+انه انا بعمل VB1 بحط بعدين بحط VB2 ممنوع احط VB2
+
+200
+00:14:26,640 --> 00:14:31,000
+بعدين احط VB1 السؤال هان why او شو هيصير لو انا
+
+201
+00:14:31,000 --> 00:14:37,830
+حطيت VB2أول issue بعدين حطيت VB1 بقول لي ال VB2 لو
+
+202
+00:14:37,830 --> 00:14:43,250
+حطيتيه بالأول مش هيرتبط مع ال Acetone تمام ال
+
+203
+00:14:43,250 --> 00:14:48,490
+Acetone فقط هيرتبط مع VB1 ال VB2 لو حطيتيه بالأول
+
+204
+00:14:48,490 --> 00:14:51,850
+مش هيرتبط بال Acetone هيروح يرتبط بال Peptone اللي
+
+205
+00:14:51,850 --> 00:14:56,000
+موجود في ال mediaلما يرتبط مع البيبتون الموجود في
+
+206
+00:14:56,000 --> 00:15:03,280
+ال media هيعطيني لون red فانا هفكر انه النتيجة
+
+207
+00:15:03,280 --> 00:15:06,940
+positive تمام فبالتالي هيعطيني false positive
+
+208
+00:15:06,940 --> 00:15:11,720
+result كان اللون ال red نتيجة ارتباط ال VB2KOH مع
+
+209
+00:15:11,720 --> 00:15:15,740
+البيبتون في ال media وليس نتيجة ارتباط ال reagent
+
+210
+00:15:15,740 --> 00:15:21,200
+مع الacetoneعشان هيك لازم احنا نحط VB1 أول بعدين
+
+211
+00:15:21,200 --> 00:15:25,940
+VB2 لو حطيت VB2 هيرتبط مع البيبتون مش هيرتبط مع ال
+
+212
+00:15:25,940 --> 00:15:30,240
+Acetone لأن ال Acetone فقط مخصص انه يرتبط مع VB1
+
+213
+00:15:30,240 --> 00:15:33,720
+هيرتبط VB2 مع البيبتون و هيعطيني اللون ال Red انا
+
+214
+00:15:33,720 --> 00:15:37,000
+هفكره انه هو النتيجة ال positive لكن هو مش النتيجة
+
+215
+00:15:37,000 --> 00:15:41,640
+ال positive هو false positive result النقطة
+
+216
+00:15:41,640 --> 00:15:47,590
+التانية المهمة في هذا الفحص انه انا لازمأقرأ
+
+217
+00:15:47,590 --> 00:15:53,590
+النتيجة بعد ما ازدش الفترة اني احط ال reagent بعد
+
+218
+00:15:53,590 --> 00:16:00,990
+72 ساعة 24 ساعة طلعتها من ال incubator ممنوع بعد
+
+219
+00:16:00,990 --> 00:16:06,050
+72 ساعة احط ال reagent هيعطيني false negative
+
+220
+00:16:06,050 --> 00:16:12,410
+result ليش؟ لأن ال acetone قلنا unstable عشان يصير
+
+221
+00:16:12,410 --> 00:16:17,210
+مستقر بيتحول ل butanediolلـ Butanediol Stable
+
+222
+00:16:17,210 --> 00:16:21,290
+فبالتالي بعد 72 ساعة بيكون الـ Acetone يتحول لـ
+
+223
+00:16:21,290 --> 00:16:24,570
+Butanediol لو أنا ضفت الرياجانت الرياجانت قلنا
+
+224
+00:16:24,570 --> 00:16:28,810
+بيرتبط فقط مع الـ Acetone فبالتالي مش هيلاقي الـ
+
+225
+00:16:28,810 --> 00:16:32,610
+Acetone لأنه اتحول لـ Butanediol فهيعطيني negative
+
+226
+00:16:32,610 --> 00:16:35,550
+result مش هيتحول اللون للون Red هيكون not changed
+
+227
+00:16:35,550 --> 00:16:39,350
+في اللون الميديا فبالتالي هيعطي false negative
+
+228
+00:16:39,350 --> 00:16:45,150
+result عشان هيك لازم انيأنا أتكون المزرعة أخد أو
+
+229
+00:16:45,150 --> 00:16:50,850
+أضيف الـReagent بعد 24 ساعة ما تزيد عن 2 مش بعد 72
+
+230
+00:16:50,850 --> 00:16:55,450
+ساعة لأن الـAcetone unstable هيتحول لـButanediol
+
+231
+00:16:55,450 --> 00:16:59,270
+والـReagent فقط بيرتبط مع الـAcetone وليس مع
+
+232
+00:16:59,270 --> 00:17:07,170
+الـButanediol فهيعطيني False Negative Result طيب
+
+233
+00:17:07,640 --> 00:17:10,380
+قلنا لو البكتيريا عملت fermentation للـGelicose
+
+234
+00:17:10,380 --> 00:17:12,740
+عملت فحص البكتيريا الـfermentation اللي اتكلمنا
+
+235
+00:17:12,740 --> 00:17:16,680
+عنه في الجزء الأول اللي هو اللي فيه فيه نار Red
+
+236
+00:17:16,680 --> 00:17:21,340
+وGelicose والدرهامي Tube هذا الفحص لو أنا عملته
+
+237
+00:17:21,340 --> 00:17:26,540
+وطلعت positive بعمل فحص الـmixed methyl red vucose
+
+238
+00:17:26,540 --> 00:17:31,660
+buscowar test لو طلعت methyl red positiveوقص
+
+239
+00:17:31,660 --> 00:17:34,580
+مستكور نيجاتيف يبقى هي بتكون سلكت ال pathway ال
+
+240
+00:17:34,580 --> 00:17:37,720
+mixed acid pathway اللي هو ناتج عملية ال
+
+241
+00:17:37,720 --> 00:17:42,160
+fermentation strong acid من الأمثلة على هذا ال
+
+242
+00:17:42,160 --> 00:17:46,940
+positive للميثايرد و negative للوقص مستكور ال E
+
+243
+00:17:46,940 --> 00:17:52,620
+.coli وقلنا كمان لبروتيا ميرابيلسلكن لو كانت
+
+244
+00:17:52,620 --> 00:17:55,940
+Methyl Red Negative و Vagas Buscar Positive يعني
+
+245
+00:17:55,940 --> 00:18:00,560
+سلكة طريق لبيوتيولين إجلايكول pathway من الأمثلة
+
+246
+00:18:00,560 --> 00:18:04,820
+على انها تكون Methyl Red Negative Vagas Buscar
+
+247
+00:18:04,820 --> 00:18:08,370
+Positive لك لبسلة و NT رابعة أكتربقول لي لو
+
+248
+00:18:08,370 --> 00:18:12,210
+البكتيريا ما عملت fermentation بالاصل للجليكوز انا
+
+249
+00:18:12,210 --> 00:18:15,250
+ممنوع ان انا اعمل اصلا فحص الـ Methyl Red-Vacous
+
+250
+00:18:15,250 --> 00:18:19,910
+-Biscuit لان لو عملت الفحص لو انا عملت الفحص هيطلع
+
+251
+00:18:19,910 --> 00:18:23,390
+negative للـ Methyl Red و negative للVacous
+
+252
+00:18:23,390 --> 00:18:26,770
+-Biscuit زي الصدومونس الصدومونس بالاصل ما بتعمل
+
+253
+00:18:26,770 --> 00:18:29,630
+fermentation جليكوز يبقى ولا هيتكون acid ولا
+
+254
+00:18:29,630 --> 00:18:34,090
+هيتكون الجحول ممنوع انا اشوف نتيجة ان الاتنين
+
+255
+00:18:34,090 --> 00:18:36,370
+positive ال Methyl Red وVacous-Biscuit لو ما
+
+256
+00:18:36,370 --> 00:18:40,170
+عملتهم لانفس الأورجانيزم ممنوع يكونوا اتنان
+
+257
+00:18:40,170 --> 00:18:44,190
+positive لازم يكون واحد لأن positive واحد negative
+
+258
+00:18:44,190 --> 00:18:47,850
+او اتنان negative لو كانت في الأصل ما بتعمل
+
+259
+00:18:47,850 --> 00:18:50,750
+Gelicose fermentation هذا ممنوع اني انا اشوفه لأن
+
+260
+00:18:50,750 --> 00:18:54,910
+البكتيريا اما بتسلك ال mixed acid pathway او ال
+
+261
+00:18:54,910 --> 00:18:59,910
+butylene glycol pathway ما بتسلك الاتنان مع بعض
+
+262
+00:19:03,410 --> 00:19:07,350
+يبقى لو جيبنا سؤال انه البريطس رياجانت used to
+
+263
+00:19:07,350 --> 00:19:11,670
+detect if the bacteria fermentation ferment in
+
+264
+00:19:11,670 --> 00:19:16,250
+acid pathway or not نحكي false البريطس اللي أنا
+
+265
+00:19:16,250 --> 00:19:21,130
+بستخدم عشان أشوف هل البكتيريا سلكت أو سلكت ال acid
+
+266
+00:19:21,130 --> 00:19:25,370
+pathway بستخدم اللي هو ال methyrid البريطس رياجانت
+
+267
+00:19:25,370 --> 00:19:31,190
+بستخدمه في البيوتانديول pathway يبقى زي ما قلنا ان
+
+268
+00:19:31,190 --> 00:19:35,300
+هذا ممنوع اني انا أشوفهالـ Media نفسها لفحص الـ
+
+269
+00:19:35,300 --> 00:19:38,280
+Methyrid و الـ Vascular لكن الاختلاف في الـ
+
+270
+00:19:38,280 --> 00:19:44,220
+Reagent الـ Reagent بيكون في الـ Mixed Acid
+
+271
+00:19:44,220 --> 00:19:47,560
+Pathway الـ Methyrid في البيوتين البيوتيولينيش
+
+272
+00:19:47,560 --> 00:19:47,860
+البيوتيولينيش البيوتيولينيش البيوتيولينيش
+
+273
+00:19:47,860 --> 00:19:48,440
+البيوتيولينيش البيوتيولينيش البيوتيولينيش
+
+274
+00:19:48,440 --> 00:19:48,660
+البيوتيولينيش البيوتيولينيش البيوتيولينيش
+
+275
+00:19:48,660 --> 00:19:50,420
+البيوتيولينيش البيوتيولينيش البيوتيولينيش
+
+276
+00:19:50,420 --> 00:19:52,220
+البيوتيولينيش البيوتيولينيش البيوتيولينيش
+
+277
+00:19:52,220 --> 00:19:57,860
+البيوتيولينيش البيوتيولينيش البيوتيولينيش
+
+278
+00:19:57,860 --> 00:20:00,820
+البيوتيولينيش البيوتيول
+
+279
+00:20:01,770 --> 00:20:05,550
+لو شوفت عملت الفحص وضفت الـ Parity reagent هتكون
+
+280
+00:20:05,550 --> 00:20:09,690
+positive للواقع سبسكوار لكن الفحص ال fermentation
+
+281
+00:20:09,690 --> 00:20:14,480
+قلنا النتيجة ال positive بتكون لون yellowهكذا نكون
+
+282
+00:20:14,480 --> 00:20:18,000
+خلصنا تقريبا أهم الأشياء اللي لازم نعرفها في فحص
+
+283
+00:20:18,000 --> 00:20:22,240
+الميثايريد فوكس بوسكواريه هنجي لسلايدات نمر عليها
+
+284
+00:20:22,240 --> 00:20:26,260
+بقول فحص الميثايريد some coliform will ferment
+
+285
+00:20:26,260 --> 00:20:30,200
+that extracts the dextrose اللي هو الجليكوز to
+
+286
+00:20:30,200 --> 00:20:33,720
+acid product that will cause the pH to drop below
+
+287
+00:20:33,720 --> 00:20:38,040
+pH 5 this is called a mixed acid fermentation قلنا
+
+288
+00:20:38,040 --> 00:20:41,280
+انه some coliform مش كل ال .. ال .. الcoliform
+
+289
+00:20:41,280 --> 00:20:45,080
+بتعمل fermentation لل dextrose لالـ Strong Acid
+
+290
+00:20:45,080 --> 00:20:49,480
+فقط الـ E.coli من الـ من الكوليفورم هي positive
+
+291
+00:20:49,480 --> 00:20:53,720
+لالميثايل Red بتسلق الـ Mixed Acid Fermentation
+
+292
+00:20:53,720 --> 00:20:57,800
+هذا هينتج Strong Acid هيعمل على لحفظ الـ pH بصورة
+
+293
+00:20:57,800 --> 00:21:03,760
+كبيرة high decrease in pH لأقل حتى من خمسة حتى أقل
+
+294
+00:21:03,760 --> 00:21:08,260
+هيكون من 4.4 بعد ال incubation بضيف مين بضيف ال
+
+295
+00:21:08,260 --> 00:21:11,700
+reaction للميثايل Red لفحص الـ Mixed Acid
+
+296
+00:21:11,700 --> 00:21:15,670
+Fermentationهذا الـ Methyl Red بقول هيتحول للون
+
+297
+00:21:15,670 --> 00:21:21,290
+أحمر في حال كان في عندي acid product هذا بيدل أنه
+
+298
+00:21:21,290 --> 00:21:24,690
+صار في عندي fermentation mixed acid fermentation
+
+299
+00:21:24,690 --> 00:21:29,810
+بنلاحظ أن اللون هانتحول للون أحمر بعد ما أنا أضفت
+
+300
+00:21:29,810 --> 00:21:33,130
+ال reaction ل Methyl Red يبقى البكتيريا كانت E
+
+301
+00:21:33,130 --> 00:21:37,250
+-coli ال positive لهذا الفحص Methyl Red E-coli ال
+
+302
+00:21:37,250 --> 00:21:40,610
+Antirobacter قلنا negative مافي بيصير أي تغير في
+
+303
+00:21:40,610 --> 00:21:44,020
+لون اللي نديها إذا ملاحظين highuninculated مش
+
+304
+00:21:44,020 --> 00:21:47,800
+مزروعهاك بتكون لون الميديا مافي اي تغير في لون
+
+305
+00:21:47,800 --> 00:21:50,980
+الميديا يبقى هي negative زي ال Eclipsilla و ال
+
+306
+00:21:50,980 --> 00:21:55,320
+Enterobacter Vagas-Boschauer Test و for other
+
+307
+00:21:55,320 --> 00:22:00,740
+coliform البكتيريا اللى ماعملت اللى هو ال mix acid
+
+308
+00:22:00,740 --> 00:22:05,580
+fermentation اكيد هتعمل ال Vagas-Boschauer Test او
+
+309
+00:22:05,580 --> 00:22:11,620
+ال butanediol pathwayهتحول الـ dextrose الجليكوز
+
+310
+00:22:11,620 --> 00:22:16,160
+to less acidic product لـ product أقل حمضية الكحول
+
+311
+00:22:16,160 --> 00:22:20,780
+product زي ال ethanol or butanediol هذه البكتيريا
+
+312
+00:22:20,780 --> 00:22:24,040
+بقولي بتكون negative في فحص الميثايريد لو أنا عملت
+
+313
+00:22:24,040 --> 00:22:27,220
+فحص الميثايريد هتكون negative في الميثايريد لكن
+
+314
+00:22:27,220 --> 00:22:31,300
+positive في الفاكسبوسكاالـ Butanediol fermentation
+
+315
+00:22:31,300 --> 00:22:35,560
+الـ pathway هذا قلناه أنا بدي أقدر أكشف عنه بالـ
+
+316
+00:22:35,560 --> 00:22:39,920
+Vagus Piscolar Test هيقصلي وجود الـ Acetone لأنه
+
+317
+00:22:39,920 --> 00:22:43,660
+احنا قلنا الـ Reagent VB1 بيرتبط بالـ Acetone مش
+
+318
+00:22:43,660 --> 00:22:47,240
+بالـ Butanediol يبقى هو هيكشف عن وجود الـ Acetone
+
+319
+00:22:47,240 --> 00:22:51,980
+اللي هو بيكون أساسي لـ Butanediol الـ Butanediol
+
+320
+00:22:51,980 --> 00:22:55,960
+الـ Acetone بيتحول لـ Butanediol الـ Butanediol هو
+
+321
+00:22:55,960 --> 00:22:58,120
+stable neutral
+
+322
+00:23:00,050 --> 00:23:04,910
+التي نان الفحصين الـ methyl red test و ال podcast
+
+323
+00:23:04,910 --> 00:23:07,690
+prescribed test بستخدم نفس ال media لأن ال
+
+324
+00:23:07,690 --> 00:23:11,430
+substrate نفسها هي الجليكوز لكن ال reagent بيختلف
+
+325
+00:23:11,430 --> 00:23:17,700
+بضيف بعض ال incubation بقولي أن أنا الفحصينبعملهم
+
+326
+00:23:17,700 --> 00:23:24,740
+بنفس الوقت عشان أشوف هل عشان أشوف يعني إذا هي أكيد
+
+327
+00:23:24,740 --> 00:23:28,640
+كانت positive لواحد هتكون negative للتانية عشان
+
+328
+00:23:28,640 --> 00:23:32,480
+أتأكد أنه واحدة positive والتانية لازم تكون
+
+329
+00:23:32,480 --> 00:23:37,460
+negative بارتس رياجونت البارتس رياجونت عشان أكشف
+
+330
+00:23:37,460 --> 00:23:42,920
+على ال focus buscler test قلنا ال VB1 5% αنفثالالـ
+
+331
+00:23:42,920 --> 00:23:47,460
+VB2 اللى هو 40% بوتاسيوم هيدروكسايد الـ KOH بضيفه
+
+332
+00:23:47,460 --> 00:23:54,480
+بعد 24 ساعة to 24 hour culture قولنا بعد ما تكون
+
+333
+00:23:54,480 --> 00:23:59,080
+المزرعة قعدت incubation 24 ساعة ممنوع اني احطها
+
+334
+00:23:59,080 --> 00:24:03,900
+بعد 72 ساعة الـ tube بعملها شاكن بحاول اني انا
+
+335
+00:24:03,900 --> 00:24:09,890
+اعمل شاكن عشان انه يصير mixبتحول اللون للون pink
+
+336
+00:24:09,890 --> 00:24:14,770
+او red بعد ما انا اعمل checking هذا بيدل على ال
+
+337
+00:24:14,770 --> 00:24:18,430
+positive reaction نتيجة وجود اللي هو ال Acetone
+
+338
+00:24:18,430 --> 00:24:22,410
+وليس Libyutanediol لإنه احنا قلنا ال reagent
+
+339
+00:24:22,410 --> 00:24:25,850
+بيرتبط مع ال Acetone وليس Libyutanediol عشان هيك
+
+340
+00:24:25,850 --> 00:24:32,490
+لازم ال culture تكون ما اتزيد عن 72 ساعة ملاحظها
+
+341
+00:24:32,490 --> 00:24:37,100
+لفوكاس بوسكوار بيكون لون هقولنا أصفرهذه ليست
+
+342
+00:24:37,100 --> 00:24:40,860
+مزروعة، هي نفسها لـ Methyl Arid الـ Positive،
+
+343
+00:24:40,860 --> 00:24:43,860
+نلاحظ أنه فيه أنجلون أحمر قلنا الـ Positive في
+
+344
+00:24:43,860 --> 00:24:46,420
+الفوكس بوسكوار هي الـ Enterobacter و الـ
+
+345
+00:24:46,420 --> 00:24:49,000
+Eclipsella اللي كانت Negative في الـ Methyl Arid
+
+346
+00:24:49,000 --> 00:24:51,120
+هي هذه الـ Eclipsella pneumonia والـ Enterobacter
+
+347
+00:24:51,120 --> 00:24:54,970
+Positive في فحص الفوكس بوسكوارلكن الـ E.coli و
+
+348
+00:24:54,970 --> 00:24:58,690
+البروتيز قلنا هيكونوا في فحص الفوكاسبوسكور نيجاتيف
+
+349
+00:24:58,690 --> 00:25:03,070
+ما تغير لون ال media هم لأنهم positive في
+
+350
+00:25:03,070 --> 00:25:06,210
+الميثايريد لأنهم بيعملوا mixed acid fermentation
+
+351
+00:25:06,210 --> 00:25:10,730
+فعلا في بعض الملاحظات اللي حكيناها برضه لو
+
+352
+00:25:10,730 --> 00:25:14,530
+البكتيريا ما بتعمل fermentation بالأصل للجليكوز لو
+
+353
+00:25:14,530 --> 00:25:17,790
+أنا عملت هذا الفحص الميثايريد و الفوكاسبوسكور
+
+354
+00:25:17,790 --> 00:25:22,930
+هتكون negative في الاتنين زي الصدومانسبقولّي ضروري
+
+355
+00:25:22,930 --> 00:25:28,190
+أنك انت تحطي الـreagent بشكل متسلسل لو ضفت الـKOH
+
+356
+00:25:28,190 --> 00:25:31,590
+اللي هو الـVB2 بالأول بقولّي هيتفاعل مع البيبتوني
+
+357
+00:25:31,590 --> 00:25:36,330
+الموجود في الـmedia هيعطيني Salmon pink color اللي
+
+358
+00:25:36,330 --> 00:25:40,390
+هو لون السمك السلمون فبالتالي أنا هفكره هو
+
+359
+00:25:40,390 --> 00:25:43,830
+الـpositive result لأنه الـpositive result بتكون
+
+360
+00:25:43,830 --> 00:25:48,790
+عندى لون أحمرلكن هذا false positive result KOH
+
+361
+00:25:48,790 --> 00:25:53,350
+اللي بيرتبط مع البيبتون لكن احنا قلنا ال reagent
+
+362
+00:25:53,350 --> 00:25:57,470
+ال VB1 بيرتبط مع الacetylene عشان يعطيني اللون
+
+363
+00:25:57,470 --> 00:26:01,470
+الأحمر VB test قلنا ال focus discover لازم تكون ال
+
+364
+00:26:01,470 --> 00:26:05,450
+culture ممنوع اني انا اعملها بعد 72 ساعة of
+
+365
+00:26:05,450 --> 00:26:11,630
+incubation لأنه هيعطيني weekly positive or false
+
+366
+00:26:11,630 --> 00:26:16,450
+negative result ليش لأنه احنا قلنا انهبعد 72 ساعة
+
+367
+00:26:16,450 --> 00:26:20,430
+الـ Acetone الـ Unstable بيتحول لمركّب
+
+368
+00:26:20,430 --> 00:26:24,150
+البيوتانديول الستابل وقلنا إن الـ Reagent الـ V.1
+
+369
+00:26:24,150 --> 00:26:28,110
+بيرتبط مع ال Acetone وليس مع البيوتانديول فلو أنا
+
+370
+00:26:28,110 --> 00:26:33,130
+حطيت بعد 72 ساعة هيكون اتحول لبيوتانديول هييجي
+
+371
+00:26:33,130 --> 00:26:36,370
+يدور على ال Acetone مش هيلاقيه فبالتالي مش هيرتبط
+
+372
+00:26:36,370 --> 00:26:40,870
+معاه ما هيعطيني اللون ال Red فهفكر أنا النتيجة هي
+
+373
+00:26:40,870 --> 00:26:44,640
+Negative لكن هي False Negative Resultلأني أنا
+
+374
+00:26:44,640 --> 00:26:48,900
+استخدمت مزرعة بعد 72 ساعة من الـ incubation حيث
+
+375
+00:26:48,900 --> 00:26:52,940
+بنكون خلصنا فحص الـ Methyl Red Vox Biscuit Test
+
+376
+00:26:52,940 --> 00:26:57,940
+هذا الفحص بعمله فقط لو كانت البكتيريا بالاصل كانت
+
+377
+00:26:57,940 --> 00:27:01,540
+fermentation جليكوز fermenter بتعمل fermentation
+
+378
+00:27:01,540 --> 00:27:09,860
+لجليكوز هذا الفحص برضه بستخدمه لتفريق في الـ
+
+379
+00:27:09,860 --> 00:27:16,140
+coliform بكتيريا في أول slide نسينانمر عليها
+
+380
+00:27:16,140 --> 00:27:19,940
+الميثايردفوكاس بوسكوهر إبقاش significant لهذا
+
+381
+00:27:19,940 --> 00:27:22,560
+الفحص used to differentiate between enteric
+
+382
+00:27:22,560 --> 00:27:25,640
+bacilli اللي هي الكوليفارم اللي قلنا انتروباكتر
+
+383
+00:27:25,640 --> 00:27:30,360
+ستروباكتر إيكولايكليبسيلا مع الاندول والسايتريت
+
+384
+00:27:30,360 --> 00:27:33,840
+السايتريت قلنا باستخدمه عشان أفرق الكوليفارم
+
+385
+00:27:33,840 --> 00:27:37,340
+بكتيريا كانت ال إيكولاي نيجاتيف والباقي positive
+
+386
+00:27:38,030 --> 00:27:41,830
+بستخدم برضه فحص الـ Methyrid Focus Biscuer و ال
+
+387
+00:27:41,830 --> 00:27:44,890
+Endol هدول الأربع فحصات عشان أفرق الـ Coliform
+
+388
+00:27:44,890 --> 00:27:49,110
+بكتيريا some of these pathway produce unstable
+
+389
+00:27:49,110 --> 00:27:52,790
+acidic product بقولي بعض أنواع البكتيريا بتسلق
+
+390
+00:27:52,790 --> 00:27:58,350
+سلوك اللي هو الـ butanediol pathway بتنتج unstable
+
+391
+00:27:58,350 --> 00:28:02,180
+acidic productwhich quickly convert to neutral
+
+392
+00:28:02,180 --> 00:28:05,240
+compound هتتحول ل neutral compound عشان هيك انا
+
+393
+00:28:05,240 --> 00:28:08,640
+سميت ال pathway ب neutral compound some organism
+
+394
+00:28:08,640 --> 00:28:13,120
+use the butylene glycol pathway هاي البكتيريا
+
+395
+00:28:13,120 --> 00:28:17,200
+بتستخدم ال butylene glycol pathway اللي بتنتج
+
+396
+00:28:17,200 --> 00:28:20,920
+الكحول which produce neutral end product بتكون
+
+397
+00:28:20,920 --> 00:28:24,960
+neutral end product including acetylene and 2,3
+
+398
+00:28:24,960 --> 00:28:29,840
+-butanediolنواتج عملية الـ fermentation الكحول
+
+399
+00:28:29,840 --> 00:28:34,520
+الاسيتوين أونستابللكن البيوتانديول هو الستابل
+
+400
+00:28:34,520 --> 00:28:38,760
+other organism ال organism الآخر قولنا انه بتسلق
+
+401
+00:28:38,760 --> 00:28:42,160
+ال mixed acid pathway هتنتج acid in the product
+
+402
+00:28:42,160 --> 00:28:45,740
+قولنا strong acid زي ال lactic acid ال acidic acid
+
+403
+00:28:45,740 --> 00:28:49,260
+and formic acid هاي ال acidic in the product قولنا
+
+404
+00:28:49,260 --> 00:28:53,960
+هي stable على عكس هي كانت unstable و بتظلها acidic
+
+405
+00:28:53,960 --> 00:28:57,980
+strong acidic هتعمل على ال high decrease in pH لكن
+
+406
+00:28:57,980 --> 00:29:02,160
+هانهتعمل slide decrease للـ pH فعشان هيك أنا بضيف
+
+407
+00:29:02,160 --> 00:29:06,740
+reaction عشان يرتبط مع النواتج وخاصة الـ Acetone
+
+408
+00:29:06,740 --> 00:29:10,960
+يعني ال VB1 بيرتبط بالـ Acetone هيك بنكون خلصنا
+
+409
+00:29:10,960 --> 00:29:15,320
+الفحص الميثايريد فوكاس بوسكوار وخلصنا فحص ال
+
+410
+00:29:15,320 --> 00:29:19,440
+fermentation البكتيريا ال fermentation وميثايريد
+
+411
+00:29:19,440 --> 00:29:22,160
+فوكاس بوسكوار قلنا الميثايريد فوكاس بوسكوار إيه له
+
+412
+00:29:22,160 --> 00:29:25,420
+علاقة في ال fermentation عشان هيك لازم يكونوا
+
+413
+00:29:25,420 --> 00:29:30,390
+اتنين مع بعضيعطيكم العافية جميعًا والسلام عليكم
+
+414
+00:29:30,390 --> 00:29:31,770
+ورحمة الله وبركاته
+
diff --git a/PL9fwy3NUQKwZWgAb_V1ATlRRsH-6i3cPC/hwINTbSvNaQ_raw.srt b/PL9fwy3NUQKwZWgAb_V1ATlRRsH-6i3cPC/hwINTbSvNaQ_raw.srt
new file mode 100644
index 0000000000000000000000000000000000000000..83755e0f9de3a4b63e44dcba6dc5aa6b00662e3b
--- /dev/null
+++ b/PL9fwy3NUQKwZWgAb_V1ATlRRsH-6i3cPC/hwINTbSvNaQ_raw.srt
@@ -0,0 +1,308 @@
+1
+00:00:00,670 --> 00:00:04,490
+في الأسبوع السابع والتامن والتاسع والعاشر هذه
+
+2
+00:00:04,490 --> 00:00:08,670
+الأسبوع كلها هناخد ال biochemical test الفحوصات
+
+3
+00:00:08,670 --> 00:00:13,250
+الكيميائية أنا عشان أوصل لتشخيص الصح لازم أجري هذه
+
+4
+00:00:13,250 --> 00:00:17,320
+الفحوصاتقبل ما اجري اي فحص لازم يكون فيه اندي انا
+
+5
+00:00:17,320 --> 00:00:21,300
+pure culture طبعا ال pure culture طبعا احنا قلنا
+
+6
+00:00:21,300 --> 00:00:26,220
+انه كل المستعمرة على الطبق ماخدة نفس الشكل اي انه
+
+7
+00:00:26,220 --> 00:00:31,280
+اصل الكولوني هو خلية بكتيرية واحدة هنتعرف على كل
+
+8
+00:00:31,280 --> 00:00:36,500
+فحص بالتفصيل هنتعرف على ال principle الفحص هنتعرف
+
+9
+00:00:36,500 --> 00:00:40,920
+علىالـ Significant أهمية كل فحص، كيف بنعمل الفحص،
+
+10
+00:00:40,920 --> 00:00:43,840
+شو النتيجة الـ Positive، كيف بتكون، شو النتيجة الـ
+
+11
+00:00:43,840 --> 00:00:47,500
+Negative، كيف بتكون شكلها، مين الـ Positive لهذا
+
+12
+00:00:47,500 --> 00:00:51,120
+الفحص من البكتيريا ومين الـ Negative لكل فحص من
+
+13
+00:00:51,120 --> 00:00:55,180
+البكتيريا بالنسبة للأسبوع الـ 11، في الأسبوع الـ
+
+14
+00:00:55,180 --> 00:00:58,980
+11 هنتعرف على الـ Bacterial Oxygen Requirement and
+
+15
+00:00:58,980 --> 00:01:02,640
+Anaerobic Bacteriaطبعا البكتيريا منصنفها حسب
+
+16
+00:01:02,640 --> 00:01:06,620
+حجبتها لأكسوجين بكتيريا هوائية بكتيريا لا هوائية
+
+17
+00:01:06,620 --> 00:01:10,820
+بكتيريا بتحتها الـCO2 مثلا فبالتالي انا لما بدي
+
+18
+00:01:10,820 --> 00:01:14,160
+اعزل بكتيريا X وانا عارفة ان بكتيريا X بتحتها
+
+19
+00:01:14,160 --> 00:01:17,900
+الـCO2 فلازم اوفر ظروف الـCO2 حتى تنمو لو انا
+
+20
+00:01:17,900 --> 00:01:21,240
+ماوفرت هذه الظروف البكتيريا مش هتنمو فهذا المعمل
+
+21
+00:01:21,240 --> 00:01:26,040
+هنتعرف كيف نوفر مثلا ظروف هوائية كيف نوفر ظروف لا
+
+22
+00:01:26,040 --> 00:01:30,070
+هوائية كيف انا بدي أوفر ظروف CO2في الأسبوع الـ 12
+
+23
+00:01:30,070 --> 00:01:34,810
+هنتعرف single media multiple test طبعا هذا يتبع لل
+
+24
+00:01:34,810 --> 00:01:38,890
+biochemical test انا في ال biochemical test لو بدي
+
+25
+00:01:38,890 --> 00:01:43,350
+اعمل مثلا عشان اوصل لتشخيص الصحة اكيد هعمل اكتر من
+
+26
+00:01:43,350 --> 00:01:47,010
+فحص كيميائي لان كل ما زادت عدد الفحوصات كل ما زادت
+
+27
+00:01:47,010 --> 00:01:52,610
+الدقة في التشخيص تخيلوا انا بديأشخص بكتيريا معينة
+
+28
+00:01:52,610 --> 00:01:57,850
+و بدي اعمل فحص كذا فحص بالتالي هياخد مني وقت و
+
+29
+00:01:57,850 --> 00:02:02,290
+هياخد مني مجهود ان انا كل فحص بده أدوات خاصة بدي
+
+30
+00:02:02,290 --> 00:02:07,250
+احضر لكل فحص مثلا media خاصة هذا هياخد مني وقت و
+
+31
+00:02:07,250 --> 00:02:10,870
+جهدفكرة الـ Single Media Multiple Test انه ميديا
+
+32
+00:02:10,870 --> 00:02:15,670
+واحدة بزرع البكتيريا اللي انا بدي طبعا اعرف او بدي
+
+33
+00:02:15,670 --> 00:02:20,030
+اشخصها و اعرف هويتها و تاني يوم باجي بشوف النتائج
+
+34
+00:02:20,030 --> 00:02:25,850
+بكون طبعا النتيجة بعد 24 ساعة بقرأ نتيجة طبعا اكتر
+
+35
+00:02:25,850 --> 00:02:30,090
+من فحص في نفس الميديا في الأسبوع التلتاشر في اشي
+
+36
+00:02:30,090 --> 00:02:33,030
+اسمه هناخد معملين طبعا Selective and Differential
+
+37
+00:02:33,030 --> 00:02:36,390
+Media وفي معمل تاني منفصل عنه اللي هو Serial
+
+38
+00:02:36,390 --> 00:02:39,870
+Dilutionبالنسبة لـ Selective and Differential
+
+39
+00:02:39,870 --> 00:02:42,990
+Media قولنا ميديا هو وسط غذائي الوسط الغذائي في
+
+40
+00:02:42,990 --> 00:02:45,570
+عندنا إيه اللي هو تصنيفات مختلفة هنتعرف في هذا
+
+41
+00:02:45,570 --> 00:02:49,070
+المعمل عن هذه التصنيفات في من ضمن التصنيفات إش
+
+42
+00:02:49,070 --> 00:02:52,450
+اسمه اللي هو الـ Selective Media الـ Selective
+
+43
+00:02:52,450 --> 00:02:58,840
+معناها اللي هي انتقائي أو اختيارييعني أنا بدي أنمي
+
+44
+00:02:58,840 --> 00:03:03,480
+مثلا الـ bacteria اللي هي الـ Mojapetgram في عندي
+
+45
+00:03:03,480 --> 00:03:07,960
+ميديا خاصة بتنمي فقط الـ Mojapetgram و بتطبط الـ
+
+46
+00:03:07,960 --> 00:03:11,320
+Celebitgram طبعا أكيد في الميديا في مكون بيعمل على
+
+47
+00:03:11,320 --> 00:03:16,540
+تثبيت مثلا الـ Celebitgramهنتعرف على أنواع متعددة
+
+48
+00:03:16,540 --> 00:03:19,760
+من الـ Media Selective الـ Differential Media إن
+
+49
+00:03:19,760 --> 00:03:24,440
+هي بتنمي أكتر منه لكن بتعطي مثلا بكتيريا X لون على
+
+50
+00:03:24,440 --> 00:03:29,440
+هذا الوسط وبتعطي بكتيريا Y لون تاني على نفس الوسط
+
+51
+00:03:29,440 --> 00:03:33,140
+يمكن شفته في صورة الـ group الـ WhatsApp فيه
+
+52
+00:03:33,140 --> 00:03:38,500
+انديانطبق مقسم نصين نصه مزروع عليه بكتيريا X هو
+
+53
+00:03:38,500 --> 00:03:43,770
+النص التاني مزروع عليه بكتيريا YX أعطت لون أصفر وY
+
+54
+00:03:43,770 --> 00:03:46,590
+أعطت لون colorless فهذه تعتبر الـ differential
+
+55
+00:03:46,590 --> 00:03:50,570
+media الـ cereal dilution طبعا احنا قلنا ان
+
+56
+00:03:50,570 --> 00:03:56,750
+البكتيريا تعتبر ما بشوفها الا على شكل مستعمرات
+
+57
+00:03:56,750 --> 00:04:01,350
+فبالتالي انا لو بدي اعد البكتيريا مش هقدر اعد
+
+58
+00:04:01,350 --> 00:04:05,370
+البكتيريا لأن الكلوني هو تجمع ملايين من الخلايا
+
+59
+00:04:05,370 --> 00:04:08,910
+البكتيرية في هذا المعمل هنتعرف كيف انا لو بدي اعد
+
+60
+00:04:08,910 --> 00:04:12,510
+البكتيريا مثلا بدي اشوفمدى استجابة المريض للـ
+
+61
+00:04:12,510 --> 00:04:16,170
+Antibiotic هلقل ولا زاد هنعرف من خلال الـ Serial
+
+62
+00:04:16,170 --> 00:04:20,590
+Dilution في الأسبوع الـ 14 هنعرف لـ Growth Curve
+
+63
+00:04:20,590 --> 00:04:24,290
+and Microbial Control Agents هنتعرف على نمو
+
+64
+00:04:24,290 --> 00:04:28,070
+البكتيريا شو العوامل اللتي بتأثر على النمو بشكل
+
+65
+00:04:28,070 --> 00:04:36,570
+عام كيف بدي أقيس مدى النمو هذا طبعا مواضيع المساق
+
+66
+00:04:36,570 --> 00:04:42,170
+ويتوزيها خلال الفصلبالنسبة لتوزيع الدرجات هيكون
+
+67
+00:04:42,170 --> 00:04:47,370
+طبعا تلاتين و نص في و سبعين نهائي في عندي quizات
+
+68
+00:04:47,370 --> 00:04:51,550
+عشرين درجة هيكون quizان كل quiz هيكون في تلات
+
+69
+00:04:51,550 --> 00:04:56,390
+معامل كل quiz هيكون من عشرين و هنقسم الدرجة على
+
+70
+00:04:56,390 --> 00:05:02,090
+اتنين عشرة بيطلع عشرة في لكل quiz homework عليا
+
+71
+00:05:02,090 --> 00:05:06,010
+عشر علامات هيكون homework واحد فقط هنحكي عنه في
+
+72
+00:05:06,010 --> 00:05:09,130
+حينه هيكون شويه يعنيبعد ما نخلص الفحوزات
+
+73
+00:05:09,130 --> 00:05:15,250
+الكيميائية ال final exam هيكون سبعين تلاتين عملي و
+
+74
+00:05:15,250 --> 00:05:20,250
+أربعين نظري بالنهاية طبعا مجموع العلمات مية هيك
+
+75
+00:05:20,250 --> 00:05:26,150
+بيكون توزيع الدرجات بتمنى تكون الأمور واضحة و
+
+76
+00:05:26,150 --> 00:05:29,590
+بتمنى لكوا كل التوفيق والسلام عليكم ورحمة الله
+
+77
+00:05:29,590 --> 00:05:30,450
+وبركاته
+
diff --git a/PL9fwy3NUQKwZWgAb_V1ATlRRsH-6i3cPC/kghd40S327U_postprocess.srt b/PL9fwy3NUQKwZWgAb_V1ATlRRsH-6i3cPC/kghd40S327U_postprocess.srt
new file mode 100644
index 0000000000000000000000000000000000000000..cb27db09cac567f061017b4ab3fb055f4b6a2d05
--- /dev/null
+++ b/PL9fwy3NUQKwZWgAb_V1ATlRRsH-6i3cPC/kghd40S327U_postprocess.srt
@@ -0,0 +1,1796 @@
+1
+00:00:02,530 --> 00:00:06,450
+السلام عليكم ورحمة الله وبركاته يعطيكم العافية
+
+2
+00:00:06,450 --> 00:00:10,410
+جميعا في هذا اللقاء هتكون المحاضرة بعنوان الـ
+
+3
+00:00:10,410 --> 00:00:13,790
+Serial Dilution Method of Bacteria Enumeration
+
+4
+00:00:13,790 --> 00:00:18,870
+طريقة عد البكتيريا باستخدام الـ Serial Dilution
+
+5
+00:00:18,870 --> 00:00:23,710
+Method طبعا هنذكر أكثر من طريقة لعد البكتيريا لكن
+
+6
+00:00:23,710 --> 00:00:27,550
+في هذا المعمل هنشرح طريقة الـ Serial Dilution
+
+7
+00:00:27,550 --> 00:00:31,490
+Method many studies require the quantitative
+
+8
+00:00:32,150 --> 00:00:35,470
+determination of bacterial population واللي في
+
+9
+00:00:35,470 --> 00:00:39,950
+كثير من الدراسات بتحتاج ان انا اعد البكتيريا
+
+10
+00:00:39,950 --> 00:00:43,410
+quantitative determination of bacterial population
+
+11
+00:00:43,410 --> 00:00:48,410
+quantitative يعني عد البكتيريا لكن لما انا احكي
+
+12
+00:00:48,410 --> 00:00:53,170
+qualitative qualitative يعني presence or absence
+
+13
+00:00:53,170 --> 00:00:56,790
+بدي اشوف هل البكتيريا موجودة ولا لأ لكن
+
+14
+00:00:56,790 --> 00:01:01,340
+quantitative بتكلم على عد البكتيريابقول في عندي
+
+15
+00:01:01,340 --> 00:01:05,540
+طريقتين لعد البكتيريا the two most widely used
+
+16
+00:01:05,540 --> 00:01:10,240
+method for determining bacterial numbers are
+
+17
+00:01:10,240 --> 00:01:14,540
+الطريقتين اللي أنا بستخدمهم في عد البكتيريا ال
+
+18
+00:01:14,540 --> 00:01:18,560
+most common أول طريقة اللي هي ال standard blood
+
+19
+00:01:18,560 --> 00:01:22,820
+count method اللي هنتكلم عنها في هذا المعمل اللي
+
+20
+00:01:22,820 --> 00:01:27,300
+هي cereal dilution method الطريقة التانية ال
+
+21
+00:01:27,300 --> 00:01:31,880
+spectrophotometerالـ Terbidiometric Analysis
+
+22
+00:01:31,880 --> 00:01:37,040
+بتعتمد على الـ Terbidity طبعا هذه الطريقة هتذكرها
+
+23
+00:01:37,040 --> 00:01:41,800
+في ال chapter اللي معنوه في chapter اللي هو ال
+
+24
+00:01:41,800 --> 00:01:44,040
+bacterial growth اللي هي طريقة الـ
+
+25
+00:01:44,040 --> 00:01:49,160
+Spectrophotometer بقول لي طريقة ال standard blood
+
+26
+00:01:49,160 --> 00:01:53,440
+count method أو Serial dilution method is an
+
+27
+00:01:53,440 --> 00:01:57,840
+indirect measurement of cell density هي طريقة
+
+28
+00:01:57,840 --> 00:01:58,620
+indirect
+
+29
+00:02:10,430 --> 00:02:14,650
+indirect measurement ليش قاللي طريقة غير مباشرة
+
+30
+00:02:14,650 --> 00:02:21,310
+لأنه في التعبير على عدد البكتيريا بكتب في نهاية
+
+31
+00:02:21,310 --> 00:02:28,070
+الحل colony farming unit الوحدة اللي أنا بعبر عنها
+
+32
+00:02:28,070 --> 00:02:33,150
+هي colony farming unit per ml أو اجرام حسب العينة
+
+33
+00:02:33,530 --> 00:02:37,430
+طيب كولوني يعني مستعمرة المستعمرة انا ما بعرف قداش
+
+34
+00:02:37,430 --> 00:02:40,930
+البكتيريا الموجودة فيها ممكن تكون single ممكن تكون
+
+35
+00:02:40,930 --> 00:02:45,570
+chain فبالتالي انا بعبر ب colony farming unit عشان
+
+36
+00:02:45,570 --> 00:02:48,890
+هيك انا بسمي هذه الطريقة او بحكي عن هذه الطريقة
+
+37
+00:02:48,890 --> 00:02:54,310
+indirect measurement طيب ليش قاللي ان هي فقط بتقيس
+
+38
+00:02:54,310 --> 00:02:58,800
+ال live bacteria بتقيس البكتيريا الحيةمنعد في ال
+
+39
+00:02:58,800 --> 00:03:02,080
+standard blood count method فقط البكتيريا الحية
+
+40
+00:03:02,080 --> 00:03:06,280
+لأنه لو ميتة أكيد مش هتتكثر لأنه بعتمد على ال
+
+41
+00:03:06,280 --> 00:03:10,620
+blood count method ال blood count method ان انا
+
+42
+00:03:10,620 --> 00:03:16,960
+بدي أزرع او أعمل تخفيف للعينة بعدين أزرع كل تخفيف
+
+43
+00:03:16,960 --> 00:03:21,800
+على طبق و أحطها في ال incubator فبالتالي البكتيريا
+
+44
+00:03:21,800 --> 00:03:26,850
+لو ميتة مش هتتكثرفي هذا الطبق عشان هيك فقط في هذه
+
+45
+00:03:26,850 --> 00:03:30,990
+الطريقة بتقيس البكتيريا أو من عد البكتيريا الحية
+
+46
+00:03:30,990 --> 00:03:36,690
+طيب الطريقة التانية لـ spectrophotometer analysis
+
+47
+00:03:36,690 --> 00:03:40,130
+بقول بتعتمد على الـ turbidity عشان هيك اسمها
+
+48
+00:03:40,130 --> 00:03:46,110
+turbidometric analysis or spectrophotometer بقول
+
+49
+00:03:46,110 --> 00:03:50,760
+بتعتمد على ال turbidityindirectly measured برضه
+
+50
+00:03:50,760 --> 00:03:54,060
+نفس ال standard blood count هي تعتبر indirect
+
+51
+00:03:54,060 --> 00:03:58,420
+measurement لماذا؟ لأنها بتعتمد على ال turbidity
+
+52
+00:03:58,420 --> 00:04:03,200
+طبعا العكارة ما بقدر اميز البكتيريا الحية
+
+53
+00:04:03,200 --> 00:04:06,520
+والبكتيريا الميتة لما يكون في انمو على شكل ال
+
+54
+00:04:06,520 --> 00:04:10,180
+growth على شكل العكارة على شكل ال turbidity ما
+
+55
+00:04:10,180 --> 00:04:14,810
+بقدر اميزهل البكتيريا حية ولا ميتة البكتيريا سواء
+
+56
+00:04:14,810 --> 00:04:18,390
+كانت حية او ميتة بيكون في عندي turbidity بيكون في
+
+57
+00:04:18,390 --> 00:04:23,630
+عندي عكارة تمام يبقى سواء كانت طبعا هي تحت ال
+
+58
+00:04:23,630 --> 00:04:27,190
+microscope البكتيريا او في عندي عكارة مابقدر اميز
+
+59
+00:04:27,190 --> 00:04:30,450
+بين البكتيريا الحية والميتة لكن على الطبق فقط
+
+60
+00:04:30,450 --> 00:04:35,070
+البكتيريا الحية هي اللي بتتكثر يبقى بطريقة ال
+
+61
+00:04:35,070 --> 00:04:38,940
+standard blood count انا بعد البكتيريا الحيةفي
+
+62
+00:04:38,940 --> 00:04:43,900
+طريقة الـ Spectrophotometer بقيس البكتيريا الحية
+
+63
+00:04:43,900 --> 00:04:50,000
+والميتة تمام هذا بالنسبة للطريقتين الطريقتين طبعا
+
+64
+00:04:50,000 --> 00:04:54,540
+هم العد البكتيريا الـ Quantitative طب نيجي لأول
+
+65
+00:04:54,540 --> 00:04:59,380
+طريقة اللى هتكون او اللى هنشرح عنها في هذا المعمل
+
+66
+00:04:59,380 --> 00:05:03,440
+اللى هى ال standard blood count method اللى بسميها
+
+67
+00:05:03,440 --> 00:05:04,820
+viable count
+
+68
+00:05:09,180 --> 00:05:13,840
+the blood count viable count يعني حية لأن هي بتعد
+
+69
+00:05:13,840 --> 00:05:16,760
+البكتيريا الحية يبقى إلها اسم تاني اللي هي ال
+
+70
+00:05:16,760 --> 00:05:21,680
+viable count لأنه بتعد فقط البكتيريا الحية the
+
+71
+00:05:21,680 --> 00:05:25,500
+number of bacteria in a given sample is usually
+
+72
+00:05:25,500 --> 00:05:30,440
+too great to be counted directly بقولي البكتيريا
+
+73
+00:05:30,440 --> 00:05:35,300
+أو عدد البكتيريا في عينة معينة بيكون كتير كبير
+
+74
+00:05:35,300 --> 00:05:39,970
+لإني أنا أقدر أعدهافبالتالي لو انت روحتي اخدتي من
+
+75
+00:05:39,970 --> 00:05:44,650
+العينة الاصلية روحتي زلعتيها على الطبق هيكون كل
+
+76
+00:05:44,650 --> 00:05:48,890
+الطبق مليان مستعمرات بكتيريا فبالتالي انا مش هقدر
+
+77
+00:05:48,890 --> 00:05:54,510
+اعد مش هعرف انا اعد هاي ال colony عشان هيك بنلجأ
+
+78
+00:05:54,510 --> 00:05:58,850
+لطريقة ال dilution however if the sample is
+
+79
+00:05:58,850 --> 00:06:03,110
+serially diluted بقولي ان العينة انا بعملي لها
+
+80
+00:06:03,110 --> 00:06:08,040
+تخفيف عشان اني انا اقدر اعدهاتمام لازم احنا نخفف
+
+81
+00:06:08,040 --> 00:06:12,080
+العينة الأصلية فبالتالي بيكون العدد قليل بقدر اني
+
+82
+00:06:12,080 --> 00:06:17,080
+انا عيدها بعدها بدرب في ال factor اللي انا اللي هو
+
+83
+00:06:17,080 --> 00:06:23,280
+شو التخفيف شو ال dilution بدرب فيه ال factor and
+
+84
+00:06:23,280 --> 00:06:27,740
+then blotted out on an agar surface in such manner
+
+85
+00:06:27,740 --> 00:06:31,020
+that single isolated bacteria from visible
+
+86
+00:06:31,020 --> 00:06:35,460
+isolated colonyبقولي بعد ما تعملي serial dilution
+
+87
+00:06:35,460 --> 00:06:40,700
+تعملي تخفيف للعينة الأصلية بروح أنا باخد من كل
+
+88
+00:06:40,700 --> 00:06:47,200
+تخفيف حجم معين تقريبا كنا ناخد اللي هو واحد من
+
+89
+00:06:47,200 --> 00:06:52,720
+عشرة ML من كل تخفيفه أروح أنا أزرعه على طبق تمام
+
+90
+00:06:52,720 --> 00:06:57,980
+بعدين أفرد هذه الحجم الواحد من عشرة ML أفرده على
+
+91
+00:06:57,980 --> 00:07:03,380
+الطبق باستخدام الالشيبممكن كنا نعمل من كل إت يوب
+
+92
+00:07:03,380 --> 00:07:08,000
+نعمل two بلات طبقين و بعد بقى أنا أعيد أخد ال
+
+93
+00:07:08,000 --> 00:07:11,320
+average بينهم ممكن أعمل على طبق واحد أزرع كل إت
+
+94
+00:07:11,320 --> 00:07:14,780
+يوب كل تخفيف على طبق واحد أو ممكن على طبقين و
+
+95
+00:07:14,780 --> 00:07:19,140
+بعدين أخد ال average بنفرضها بالالشف و بنحطها في
+
+96
+00:07:19,140 --> 00:07:23,240
+ال incubator و بعد 24 ساعة بشوف ال count بشوف
+
+97
+00:07:23,240 --> 00:07:29,030
+العدربقولي كل single isolated bacteria بتكون من
+
+98
+00:07:29,030 --> 00:07:32,870
+isolated colony بتعبر على ال isolated colony the
+
+99
+00:07:32,870 --> 00:07:36,430
+number of colony can be used as a measure of the
+
+100
+00:07:36,430 --> 00:07:40,050
+number of viable living cell in the non-dilution
+
+101
+00:07:40,050 --> 00:07:44,730
+بقولي عدد ال colony اللي أنا بدي أشوفها بعد ما
+
+102
+00:07:44,730 --> 00:07:51,190
+أحطها في ال incubator هتعبر عنعدد الخلايا الحية في
+
+103
+00:07:51,190 --> 00:07:55,010
+هذا الـ dilution في هذا التخفيف اللي أنا أخدت منه
+
+104
+00:07:55,010 --> 00:08:02,830
+وزرعته على الطبق تمام هذا بالنسبة لأول slide نيجي
+
+105
+00:08:02,830 --> 00:08:06,610
+keep in mind that if the organism normally forms
+
+106
+00:08:06,610 --> 00:08:10,210
+multiple cell arrangements such as chain the
+
+107
+00:08:10,210 --> 00:08:13,830
+colony farming unit may consist of a chain of
+
+108
+00:08:13,830 --> 00:08:18,030
+bacteria rather than a single bacteria بقوللي انه
+
+109
+00:08:18,350 --> 00:08:21,810
+أحنا قلنا في أول معمل لما تعرفنا على الشاب قلنا
+
+110
+00:08:21,810 --> 00:08:25,870
+البكتيريا ممكن تكون في عندي كمان arrangement ترتيب
+
+111
+00:08:25,870 --> 00:08:30,950
+للخلايا البكتيرية حسب مستوى الانقصام ممكن
+
+112
+00:08:30,950 --> 00:08:34,870
+البكتيريا تكون على اتشان على شكل سلسلة ممكن تكون
+
+113
+00:08:34,870 --> 00:08:39,830
+على شكل تجمعات تمام فبالتالي لما أنا أعبر بال
+
+114
+00:08:39,830 --> 00:08:43,710
+colony farming unit ال colony farming unitقلنا
+
+115
+00:08:43,710 --> 00:08:47,790
+ممكن انها تكون الكوني تجمع من الخلايا البكتيرية ال
+
+116
+00:08:47,790 --> 00:08:51,190
+single cell بقولي هان مش برضه مش ممكن انها تكون
+
+117
+00:08:51,190 --> 00:08:55,570
+على شكل single بكتيريا ممكن انها تكون chain of
+
+118
+00:08:55,570 --> 00:09:00,910
+بكتيريا فبالتالي هان انا بعتبر ال measurement
+
+119
+00:09:00,910 --> 00:09:05,300
+indirect بقولي in additionsome of the bacteria may
+
+120
+00:09:05,300 --> 00:09:09,120
+be clumped together برضه ممكن البكتيريا تتجمع مع
+
+121
+00:09:09,120 --> 00:09:12,820
+بعضها زي ما قلنا ممكن انها تكون على شكل أسطاف على
+
+122
+00:09:12,820 --> 00:09:17,480
+شكل انقود العنب فبالتالي therefore we doing the
+
+123
+00:09:17,480 --> 00:09:21,720
+blood cow technique we generally say we are
+
+124
+00:09:21,720 --> 00:09:24,800
+determining the number of colony farming unit in
+
+125
+00:09:24,800 --> 00:09:28,600
+that non dilution عشان هيك انا لما اعمل طريقة ال
+
+126
+00:09:28,600 --> 00:09:33,810
+cereal dilution or blood cow techniqueبعبّر
+
+127
+00:09:33,810 --> 00:09:38,390
+بالنهاية على العدد بـ Colony Farming Unit ما بحكي
+
+128
+00:09:38,390 --> 00:09:41,610
+Single Bacteria او Each and Bacteria لأنه بالنهاية
+
+129
+00:09:41,610 --> 00:09:46,270
+المستعمرة ممكن تكون عبارة عن Single Bacterium ممكن
+
+130
+00:09:46,270 --> 00:09:50,390
+تكون Each and ممكن تكون Clump فبعبّر على Colony
+
+131
+00:09:50,390 --> 00:09:54,770
+Farming Unit اللي هو المستعمرة عدد المستعمرات اللي
+
+132
+00:09:54,770 --> 00:10:00,480
+أناعديتها في هذا لبلات اللى كان اللى انا انا اصلا
+
+133
+00:10:00,480 --> 00:10:04,780
+زرعته من هذا in that non dilution من هذا التخفيف
+
+134
+00:10:04,780 --> 00:10:10,300
+by extrapolation this number can in turn be used
+
+135
+00:10:10,300 --> 00:10:13,420
+to calculate the number of colony forming unit in
+
+136
+00:10:13,420 --> 00:10:18,120
+the original sample طبعا انا بعد عدد بعد الكولوني
+
+137
+00:10:18,120 --> 00:10:23,000
+وبنهاية بعبر عن اللى اللى هي الوحدة بكولوني
+
+138
+00:10:23,000 --> 00:10:27,690
+forming unit في هذاin the original sample بعد ما
+
+139
+00:10:27,690 --> 00:10:31,930
+أعد تخفيف بضرب في اللي هو تخفيف العينة حتى أحصل
+
+140
+00:10:31,930 --> 00:10:37,190
+على عدد البكتيريا في العينة الأصلية bacterial
+
+141
+00:10:37,190 --> 00:10:42,570
+count by this method are usually expressed as
+
+142
+00:10:42,570 --> 00:10:46,490
+colony forming unit per millimeter بقوللي عدد
+
+143
+00:10:46,490 --> 00:10:49,930
+البكتيريا في هذه الطريقة بعبّر عنها ب colony
+
+144
+00:10:49,930 --> 00:10:55,170
+forming unitper ml او اجرام حسب نوع العينة اذا
+
+145
+00:10:55,170 --> 00:11:00,850
+كانت سائلة بعبر بي الملي ليتر واذا كانت صلبة بعبر
+
+146
+00:11:00,850 --> 00:11:04,950
+بي لجرام يبقى كله uniformic unit per ml او اجرام
+
+147
+00:11:04,950 --> 00:11:06,370
+حسب نوع العينة
+
+148
+00:11:10,090 --> 00:11:14,070
+Normally the bacterial sample is diluted by factor
+
+149
+00:11:14,070 --> 00:11:19,530
+of 10 and plated on agar بقوللي عادة احنا لما انا
+
+150
+00:11:19,530 --> 00:11:25,490
+اعمل تخفيف لعينة الاصلية بروح بعمل بمضاعفات العشرة
+
+151
+00:11:25,490 --> 00:11:30,690
+و بزراعها على الagar after incubation the number
+
+152
+00:11:30,690 --> 00:11:36,090
+of colonies on dilution plate showing between بعد
+
+153
+00:11:36,090 --> 00:11:40,560
+ما انا اعمل incubation ل الأطباق اللي اناأخدت من
+
+154
+00:11:40,560 --> 00:11:46,500
+كل تخفيف حجم معين وروحت زرعته على قبلة طبق او
+
+155
+00:11:46,500 --> 00:11:50,920
+طبقين من كل تخفيف بعد ال incubation من عد هاي ال
+
+156
+00:11:50,920 --> 00:11:55,800
+colony مين الأطباق اللي انا بدي اعدها لو كان فقط
+
+157
+00:11:55,800 --> 00:12:01,380
+كان عدد ال colony ما بين 30 ل 300 colony هو هذا
+
+158
+00:12:01,380 --> 00:12:06,700
+اللي انا بدي اعوضه بالنهاية في القانونهى بقولى
+
+159
+00:12:06,700 --> 00:12:10,900
+البلاد اللى فى بين تلاتين لتلاتمية كولونى هو اللى
+
+160
+00:12:10,900 --> 00:12:16,600
+أنا بختاره لإن هو ال significant هو اللى إله أهمية
+
+161
+00:12:16,600 --> 00:12:22,660
+طيب لو كان عدد الكولونى أقل من تلاتين كولونى فى
+
+162
+00:12:22,660 --> 00:12:28,060
+الطبق بقولى تكتبي too few to count على طول من حد
+
+163
+00:12:28,060 --> 00:12:31,420
+ما تشوفى عدد الكولونى فى الطبق أقل من تلاتين
+
+164
+00:12:31,420 --> 00:12:36,570
+مابعود فى القانون بكتب tooto few to count طيب لو
+
+165
+00:12:36,570 --> 00:12:43,610
+كان اكتر من تلاتمية ما بعده بكتب فيه ال result to
+
+166
+00:12:43,610 --> 00:12:47,630
+numerous to count يبقى لو كان اقل من تلاتين to few
+
+167
+00:12:47,630 --> 00:12:51,650
+to count اللي هو اختصار هايم او اذا كان اكتر من
+
+168
+00:12:51,650 --> 00:12:59,070
+تلاتمية بكتب to numerous to count تمام؟لكن لو كان
+
+169
+00:12:59,070 --> 00:13:03,130
+بين الـ 30 والـ 300 بعد هاي الكلوني وبالنهاية بعود
+
+170
+00:13:03,130 --> 00:13:07,290
+بالقانون اللي هندكره في ال slides الجاي هذا البلاد
+
+171
+00:13:07,290 --> 00:13:12,790
+تقريبا عندي انا اللي هو 7 او 8 كلوني فبالتالي هو
+
+172
+00:13:12,790 --> 00:13:17,670
+اقل من 30 فبكتب على طول too few to count هو مش
+
+173
+00:13:17,670 --> 00:13:21,530
+مناسب اني انا اعدهم هم نلاحظ ان هو تقريبا ما بين
+
+174
+00:13:21,530 --> 00:13:25,750
+ال 30 لل 300 فبعد هاي الكلوني قداش عددها وبالنهاية
+
+175
+00:13:26,200 --> 00:13:29,680
+بعود ال number of colony فيه قانون هنتعرف عليه
+
+176
+00:13:29,680 --> 00:13:33,580
+نلاحظ ان هذا الطبق كتير اكتر من 300 تقريبا
+
+177
+00:13:33,580 --> 00:13:37,440
+فبالتالي صعب اني انا اعده فعلى طول ما بعد بكتب 2
+
+178
+00:13:37,440 --> 00:13:42,240
+in numerous to count طيب
+
+179
+00:13:42,240 --> 00:13:48,480
+نيجي للخطوات بالنسبة للخطوات كيف انا بدي اعمل
+
+180
+00:13:48,480 --> 00:13:51,920
+serial dilution method او blood count method او
+
+181
+00:13:51,920 --> 00:13:56,460
+viable countبجيب العينة الأصلية اللي أنا بدي أشوف
+
+182
+00:13:56,460 --> 00:14:00,500
+عدد البكتيريا في هذه العينة وقلنا بدنا نعمل serial
+
+183
+00:14:00,500 --> 00:14:05,700
+dilution هلأ ممكن أنا هذه العينة أخد منها مباشرة
+
+184
+00:14:05,700 --> 00:14:09,540
+وممكن أني أنا أخففها في هذا المثال ماخد منها
+
+185
+00:14:09,540 --> 00:14:16,160
+مباشرة ماخد واحد MLوعمل طبعا التيوبات اللي هي لأ
+
+186
+00:14:16,160 --> 00:14:20,100
+التخفيف طبعا أنا بحط diluent في هذه التيوبات تمام
+
+187
+00:14:20,100 --> 00:14:24,160
+على الأغلب بحط 9 ml normal saline 9 ml normal
+
+188
+00:14:24,160 --> 00:14:28,940
+saline 9 ml normal saline ممكن عادي أستخدم مثلا 4
+
+189
+00:14:28,940 --> 00:14:34,140
+.5 ml normal saline المهم بالنهاية يكون التخفيف
+
+190
+00:14:34,140 --> 00:14:39,140
+أنا عارفة شو التخفيف اللي أنا هضرب فيهكان حاطة 9
+
+191
+00:14:39,140 --> 00:14:44,400
+ml normal saline أخد 1 ml من العينة وضافها على أول
+
+192
+00:14:44,400 --> 00:14:47,920
+tube اللي هو اللي فيه daily one 9 ml normal saline
+
+193
+00:14:47,920 --> 00:14:53,960
+طيب شو هو التخفيف في هذه ال tube التخفيف اللي هو
+
+194
+00:14:53,960 --> 00:14:58,160
+ال sample على ال sample زائد ال daily one أنا أخدت
+
+195
+00:14:58,160 --> 00:15:02,640
+1 ml يبقى ال sample 1 ml على ال sample زائد ال
+
+196
+00:15:02,640 --> 00:15:06,040
+daily one ال sample 1 زائد ال daily one 9 يبقى 1
+
+197
+00:15:06,040 --> 00:15:11,020
+على 10يبقى التخفيف عشرة أس سالب واحد في هذه ال
+
+198
+00:15:11,020 --> 00:15:15,440
+tube طيب نيجي لتخفيف العينة التانية بروح أنا باخد
+
+199
+00:15:15,440 --> 00:15:19,580
+من العينة الأولى واحد ML و بحطها في هذه ال tube
+
+200
+00:15:19,580 --> 00:15:23,840
+تخفيف هذه ال tube واحد طبعا اللي هو ال sample على
+
+201
+00:15:23,840 --> 00:15:27,240
+ال total واحد زائد تسعة يبقى واحد على عشرة عشرة أس
+
+202
+00:15:27,240 --> 00:15:31,960
+سالب واحد درب تخفيف العينة اللي قبلها اللي هي عشرة
+
+203
+00:15:31,960 --> 00:15:36,910
+أس سالب واحد يبقى عشرة أس سالب اتنيننفس الفكرة هان
+
+204
+00:15:36,910 --> 00:15:40,670
+هاخد 1ml من هايلي تيوب و اضيفها على لي تيوب التالت
+
+205
+00:15:40,670 --> 00:15:45,030
+يبقى تخفيف هايلي تيوب اللي هي واحد على واحد زائد
+
+206
+00:15:45,030 --> 00:15:48,570
+تسعة ال sample اللي انا اخدت واحد ml من هان على
+
+207
+00:15:48,570 --> 00:15:53,270
+واحد زائد تسعة الدوليوان يبقى واحد على عشرة عشرة
+
+208
+00:15:53,270 --> 00:15:58,930
+أسالب واحد هو التخفيف هايلي تيوب ضرب تخفيفالتيوب
+
+209
+00:15:58,930 --> 00:16:01,510
+اللي قبلها اللي هو كان عشرة أقصالب اتنين يبقى عشرة
+
+210
+00:16:01,510 --> 00:16:04,730
+أقصالب تلاتة بنكمل بنفس الفكرة بيطلع في هاي التيوب
+
+211
+00:16:04,730 --> 00:16:09,170
+عشرة أقصالب أربعة عشرة أقصالب خمسة عشرة أقصالب ستة
+
+212
+00:16:09,170 --> 00:16:14,810
+هيك عرفنا تخفيف كل تيوب بعد ما أعمل تخفيف في هاي
+
+213
+00:16:14,810 --> 00:16:19,490
+التيوب لازم أكون عارف أنا أو كاتبة على التيوب أنه
+
+214
+00:16:19,490 --> 00:16:23,310
+هاي والله تخفيفها عشرة أقصالب واحد هاي عشرة أقصالب
+
+215
+00:16:23,310 --> 00:16:26,780
+اتنين وعلى الطبق اللي أنا هزرع منه نفس الفكرةهكتب
+
+216
+00:16:26,780 --> 00:16:30,200
+هاي تخفيفها عشرة أقصالب واحد هاي عشرة أقصالب اتنين
+
+217
+00:16:30,200 --> 00:16:33,440
+عشرة أقصالب تلاتة عشرة أقصالب أربعة عشرة أقصالب
+
+218
+00:16:33,440 --> 00:16:37,240
+خمسة وعشرة أقصالب ستة بعد ما أعمل تخفيف
+
+219
+00:16:40,700 --> 00:16:48,580
+هو مش ذاكر المهم هاخد من كل التيوب حجم معين لازم
+
+220
+00:16:48,580 --> 00:16:52,160
+انا اكون عارفة هذا الحجم لأن بعد ما اعود هعود فيه
+
+221
+00:16:52,160 --> 00:16:56,220
+القانون على سبيل المثال هاخد 1 من 10 ML من كل
+
+222
+00:16:56,220 --> 00:16:59,920
+التيوب وهزرعه على الطبق على طبق معين ممكن قلنا
+
+223
+00:16:59,920 --> 00:17:03,820
+نعمل نزرع على كل التيوب على طبقين واخد الـAverage
+
+224
+00:17:03,820 --> 00:17:08,580
+المتوسط ناخد على سبيل المثال 1 من 10 ML من هذا
+
+225
+00:17:08,580 --> 00:17:14,090
+التيوبو بحطه على إبلات و بزرع و بفرد و هكذا في كل
+
+226
+00:17:14,090 --> 00:17:19,910
+تيوبات بعد 24 ساعة بعمل count ل .. ال .. ال ..
+
+227
+00:17:19,910 --> 00:17:26,010
+اللي هو ال .. الإبلات لكلنا طبعا هتطلع في عندي هان
+
+228
+00:17:26,010 --> 00:17:30,610
+في الأغلب أقل تخفيف اللي هي too numerous to count
+
+229
+00:17:30,610 --> 00:17:34,630
+أخر إشي هتكون too few to count لكن هان تقريبا
+
+230
+00:17:34,630 --> 00:17:37,670
+هيطلع في ال .. اللي في النص ممكن إن أنا أقدر أعدها
+
+231
+00:17:38,150 --> 00:17:42,570
+فهعيد كل كله نزل و اعوض في القانون اللى هنذكره في
+
+232
+00:17:42,570 --> 00:17:46,070
+ال slides اللى بعدي انا بقول انك انت بتنقلي طبعا
+
+233
+00:17:46,070 --> 00:17:49,590
+واحد ML من كل tube بتنقليها لل tube التانى و
+
+234
+00:17:49,590 --> 00:17:52,890
+بالاها النهاية التخفيف هو تخفيفها في تخفيف اللى
+
+235
+00:17:52,890 --> 00:17:59,490
+قبلها نفس الفكرة بعدين بتعملي طبعا بتاخدي من كل
+
+236
+00:17:59,490 --> 00:18:04,250
+tube و بتحطيه على طبق أجار مكتوب و بعدين بتعملي
+
+237
+00:18:04,250 --> 00:18:10,040
+اللى هو incubationمن 24 ل 48 ساعة بعدين بقولي
+
+238
+00:18:10,040 --> 00:18:13,080
+بتهدي ال colony في الأطباق فقط لما يكون عدد ال
+
+239
+00:18:13,080 --> 00:18:19,620
+colony من 30 ل 200 أو ل 300 colonies لو كان two
+
+240
+00:18:19,620 --> 00:18:23,820
+numerous to count أكتر من 300 بكتب two numerous to
+
+241
+00:18:23,820 --> 00:18:28,240
+count لو كان أقل من 30 بكتب two few to count هذا
+
+242
+00:18:28,240 --> 00:18:33,580
+بالنسبة للطريقة اللي أنا بشتغل عليها في هاي ال
+
+243
+00:18:33,580 --> 00:18:39,940
+slide نفس الفكرةلكن الاختلاف انه هانذاكر في ليتيوب
+
+244
+00:18:39,940 --> 00:18:43,580
+مش حاطة 9 ML احنا قولنا مش شرط اني انا احط 9 ML
+
+245
+00:18:43,580 --> 00:18:49,560
+بحط اي اي حجم بديا لكن بالنهاية لازم اكون عارف هذا
+
+246
+00:18:49,560 --> 00:18:54,640
+الحجم على اللي على اساسه هحسب ال dilution هانحطت 4
+
+247
+00:18:54,640 --> 00:18:59,860
+.5 ML من ال diluent واخد نص ML من اللي هو ليتيوب
+
+248
+00:18:59,860 --> 00:19:04,340
+يبقى نص كيف دي احسب dilution هاي ليتيوب نص علىنص
+
+249
+00:19:04,340 --> 00:19:08,700
+زائد أربعة ونص بيطلع بالنهاية اللي هو عشرة أس سالب
+
+250
+00:19:08,700 --> 00:19:13,200
+واحد طيب ليش هانحطت عشرة أس سالب اتنين تخفيفها لأن
+
+251
+00:19:13,200 --> 00:19:21,540
+هاي العينة مخففة في المثال اللي قبل في
+
+252
+00:19:21,540 --> 00:19:25,500
+المثال هدا كانت العينة الأصلية مش مخففة أخدت واحد
+
+253
+00:19:25,500 --> 00:19:30,960
+MLمن high ل tube وضفتها على ال diluent وحسبت ال
+
+254
+00:19:30,960 --> 00:19:34,480
+dilution بدون ما اضرب ب dilution ل هذه العينة لأن
+
+255
+00:19:34,480 --> 00:19:39,980
+هذه العينة مش مخففة يبقى ننتبه في السؤال هل العينة
+
+256
+00:19:39,980 --> 00:19:44,340
+الأصلية مخففة إذا مخففة لازم أضرب أول ال tube في
+
+257
+00:19:44,340 --> 00:19:48,930
+تخفيف high ل tube العينة الأصليةلكن لو مش مخففة
+
+258
+00:19:48,930 --> 00:19:53,590
+خلاص على طول زى ما ذاكر في هذا المثال على طول بعمل
+
+259
+00:19:53,590 --> 00:19:57,070
+اللى هو التخفيف بحسب التخفيف بدون ما اضرب في
+
+260
+00:19:57,070 --> 00:20:00,350
+dilution high لـ tube لأنه high لـ tube مش مخففة
+
+261
+00:20:00,350 --> 00:20:07,010
+فهذا المثال هان العينة high مخففة وذا كل تخفيفها
+
+262
+00:20:07,010 --> 00:20:12,310
+عشرة أس تالب واحد يبقى تخفيف أول tube اللى هو ال
+
+263
+00:20:12,310 --> 00:20:15,890
+sample اللى أنا أخدته نص على ال sample نص زائد
+
+264
+00:20:15,890 --> 00:20:21,110
+أربعة و نص يبقىبنهاية بطلع نص على خمسة نص على خمسة
+
+265
+00:20:21,110 --> 00:20:25,650
+واحد على عشر عشرة أسالب واحد في تخفيف ال tube اللي
+
+266
+00:20:25,650 --> 00:20:28,330
+قبلها اللي هي عشرة أسالب واحد يبقى عشرة أسالب
+
+267
+00:20:28,330 --> 00:20:32,470
+اتنين طبعا هاي ال tube تخفيفها أربع بعمل بنفس
+
+268
+00:20:32,470 --> 00:20:36,350
+الفكرة نص على نص زائد أربع ونص عشرة أسالب واحد درب
+
+269
+00:20:36,350 --> 00:20:40,230
+تخفيف هاي عشرة أسالب اتنين بنهاية بطلع عشرة أسالب
+
+270
+00:20:40,230 --> 00:20:42,530
+أربع في هاي ال tube عشرة أسالب خمسة في هاي ال tube
+
+271
+00:20:42,530 --> 00:20:48,160
+عشرة أسالب ستة في هاي ال tubeأخد من كل الـ tube نص
+
+272
+00:20:48,160 --> 00:20:53,640
+أو واحد من عشرة ML و راح زرعها في طبق تمام راح
+
+273
+00:20:53,640 --> 00:20:59,060
+زرعها في هاي الأطباق و حطها في ال incubator يبقى
+
+274
+00:20:59,060 --> 00:21:03,400
+في هذا المثال ينتبه أنه هان العينة هاي مخففة فلازم
+
+275
+00:21:03,400 --> 00:21:06,860
+أضرب أول tube في تخفيفها لكن في المثال اللي ان ذكر
+
+276
+00:21:06,860 --> 00:21:11,540
+في الأول ماكانت العينة مخففة عشان هيك ماضربتش ممكن
+
+277
+00:21:11,540 --> 00:21:16,320
+في مثال تاني ما يذكرلياللي هو هان تخفيفها عشر أقل
+
+278
+00:21:16,320 --> 00:21:23,120
+سالب واحد ممكن يقولي والله أخدت خمسة وعشرين ML من
+
+279
+00:21:23,120 --> 00:21:27,740
+الملك وضفتهم على مائتين وخمسة وعشرين من ال normal
+
+280
+00:21:27,740 --> 00:21:32,060
+saline فهان بيدي وقتها أحسب تخفيف اللي هو هاي ال
+
+281
+00:21:32,060 --> 00:21:36,000
+sample اللي بنحكي خمسة وعشرين على خمسة وعشرين زائد
+
+282
+00:21:36,000 --> 00:21:38,620
+مائتين وخمسة وعشرين اللي هو ال sample زائد ال do
+
+283
+00:21:38,620 --> 00:21:43,860
+you want فبطلع خمسة وعشرين على مائتين وخمسين25 على
+
+284
+00:21:43,860 --> 00:21:48,340
+250 اللي هي 1 على 10 اللي هي 10 أساليب واحد هيك
+
+285
+00:21:48,340 --> 00:21:54,040
+بكون حاسبة تخفيف هاي لتيوب و بكمل حل زي ما كملنا
+
+286
+00:21:54,040 --> 00:21:58,960
+يبقى ممكن ما يكون مخفف العينة ممكن يكون مخففها و
+
+287
+00:21:58,960 --> 00:22:04,200
+يذكرلك التخفيف ممكن يكون فيه تخفيف لكن مش ذاكر شو
+
+288
+00:22:04,200 --> 00:22:07,620
+قيمة ال dilution فانت بدك تحسب قيمة ال dilution من
+
+289
+00:22:07,620 --> 00:22:13,400
+القانون sample على sample زائد diluentهذا بالنسبة
+
+290
+00:22:13,400 --> 00:22:18,700
+لهذه الصورة هذه السؤال هذا الفكرة نفس اللي احنا
+
+291
+00:22:18,700 --> 00:22:22,060
+ذكرناها في الأول أخد واحد ML وضفنا قولنا كل واحد
+
+292
+00:22:22,060 --> 00:22:25,180
+قداشر ال dilution هي ذكرهم عشر أقل سالب واحد عشر
+
+293
+00:22:25,180 --> 00:22:27,860
+أقل سالب اتنين عشر أقل سالب تلات عشر أقل سالب أربع
+
+294
+00:22:27,860 --> 00:22:33,400
+عشر أقل سالب خمسة عشر أقل سالب ستةواحد من عشر ML
+
+295
+00:22:33,400 --> 00:22:42,840
+منها يليت يوبات وراح زرعهم في اللي هو الاطباق
+
+296
+00:22:42,840 --> 00:22:51,120
+وبعد 24 ساعة شاف ال colonialism طيب
+
+297
+00:22:51,120 --> 00:22:56,430
+احنا قلنا بعد 24 ساعةبحسب او بعد ال colony اذا كان
+
+298
+00:22:56,430 --> 00:22:59,990
+لبلاد ما بين تلاتين لتلاتمية colony farming unit
+
+299
+00:22:59,990 --> 00:23:03,390
+ومنعود بالقانون شهو هو القانون اللى بنعود عليه
+
+300
+00:23:03,390 --> 00:23:07,010
+بقول لي calculate the number of bacteria colony
+
+301
+00:23:07,010 --> 00:23:11,890
+farming unit per milliliter or gram of sample by
+
+302
+00:23:11,890 --> 00:23:14,930
+dividing the number of colonies كيف بدي اعد او بدي
+
+303
+00:23:14,930 --> 00:23:18,690
+احسب احنا قولنا بالنهاية لبلاد count بعبر عنها ب
+
+304
+00:23:18,690 --> 00:23:23,940
+colony farming unit per ml or gramتمام طيب كيف
+
+305
+00:23:23,940 --> 00:23:27,620
+بالنهاية بدي أحسب هاي ال .. ال measures أو كيف بدي
+
+306
+00:23:27,620 --> 00:23:31,780
+أعبر بـ Colony Farming Unit per ML Origram بقولي
+
+307
+00:23:31,780 --> 00:23:35,980
+بتقسمي ال number of colonies عدد ال colony اللي
+
+308
+00:23:35,980 --> 00:23:40,820
+أنتي عدتيه على الطبق اللي كان ما بين 30 ل 300
+
+309
+00:23:40,820 --> 00:23:44,820
+قولنا ما بعود في القانون إذا كان أقل من 30 و إذا
+
+310
+00:23:44,820 --> 00:23:52,430
+أكتر من 300بعد كده بحط على الـ Sample شو هذا الـ S
+
+311
+00:23:52,430 --> 00:23:57,030
+رمز الـ S بقول لي هو كمية العينة اللي إنتي أخدتيها
+
+312
+00:23:57,030 --> 00:24:02,810
+من الـ YouTube يعني في هذا الـ YouTube أنا روحت
+
+313
+00:24:02,810 --> 00:24:06,750
+أخدت من هذا الـ YouTube واحد من عشرة ML وحطيته في
+
+314
+00:24:06,750 --> 00:24:10,130
+البلاد وروحت فردته وزرعت قداش أنا أخدت من الـ
+
+315
+00:24:10,130 --> 00:24:17,340
+YouTube دا عوض يبقى هان واحد من عشرة هي الـ Sواحد
+
+316
+00:24:17,340 --> 00:24:20,380
+من عشراء يبقى ال number of colony عدد ال colony
+
+317
+00:24:20,380 --> 00:24:24,980
+اللي عديتها في الطبق الأساس هو أداش أنا أخدت من
+
+318
+00:24:24,980 --> 00:24:30,140
+التخفيف و روحت زراعته على الطبق ال دي اللي هي ال
+
+319
+00:24:30,140 --> 00:24:34,020
+dilution شو ال tube اللي أنا أخدت منه أداش كان ال
+
+320
+00:24:34,020 --> 00:24:37,540
+dilution أداش كان التخفيف في هذه ال tube أيو
+
+321
+00:24:37,540 --> 00:24:41,340
+number of colonies دي dilution blankالـ S هي
+
+322
+00:24:41,340 --> 00:24:45,280
+volume transferred to blood قداش حجم العينة اللي
+
+323
+00:24:45,280 --> 00:24:48,980
+أنا أخدتها من ال tube في هذا ال dilution وحطيته
+
+324
+00:24:48,980 --> 00:24:51,860
+على ال blood بالنهاية هعبّر على كل uniform units
+
+325
+00:24:51,860 --> 00:25:00,360
+per ml or اجرام طيب قبل ما نذكر هذا المثال في
+
+326
+00:25:00,360 --> 00:25:07,450
+الخطوات في الخطواتفي هذا المثال ذاكر مثلا أنه أخد
+
+327
+00:25:07,450 --> 00:25:13,750
+1ML من العينة وحطينا 9ML من الـ diluent طيب ممكن
+
+328
+00:25:13,750 --> 00:25:22,270
+ما يقوللي أنه قداش أنا مثلا اللي هو قداش أنا أخدت
+
+329
+00:25:22,270 --> 00:25:26,650
+الـ diluent ممكن ما يكون ذاكر قداش رقم الـ diluent
+
+330
+00:25:26,650 --> 00:25:30,690
+قداش اللي هو 9ML هاي مش ذاكرها أو مش ذاكر ال 1ML
+
+331
+00:25:31,540 --> 00:25:37,280
+عشان نروح يقولي ال dilution في لتيوب هاي واحد على
+
+332
+00:25:37,280 --> 00:25:43,440
+عشرة تمام ال dilution في هاي لتيوب واحد على عشرة
+
+333
+00:25:43,440 --> 00:25:49,800
+وفي هاي لتيوب ضفتي انتي اربع و نص diluent قداش ال
+
+334
+00:25:49,800 --> 00:25:54,640
+volume of sample اللي أخدتي يبقى ممكن يسألي سؤال
+
+335
+00:25:54,640 --> 00:25:59,810
+كيف نلعب الأسئلةهان يقول لي ان في اليوتيوب انا
+
+336
+00:25:59,810 --> 00:26:07,110
+أخدت 4.5 ML ديليوانت والتخفيف 1 على 10 او 10 أسالب
+
+337
+00:26:07,110 --> 00:26:11,730
+1 بقول لي ال volume of sample قداش كيف بدي أحسب ال
+
+338
+00:26:11,730 --> 00:26:16,450
+volume of sample يعني اللي هي هان هادي قداش هي مش
+
+339
+00:26:16,450 --> 00:26:21,030
+ذاكرها نيجي عشان تكون الصورة واضحة اللي هو ال
+
+340
+00:26:21,030 --> 00:26:21,390
+diligent
+
+341
+00:26:33,560 --> 00:26:37,800
+in a tube اربعة
+
+342
+00:26:37,800 --> 00:26:41,920
+و نص ML
+
+343
+00:26:41,920 --> 00:26:50,800
+ال dilution اللي
+
+344
+00:26:50,800 --> 00:26:56,480
+هو واحد على بقولي
+
+345
+00:26:56,480 --> 00:27:02,080
+ال volume of
+
+346
+00:27:03,240 --> 00:27:11,680
+سامبل انت بدك تجيبى قداش هو كيف انا بدى اجيبه هلأ
+
+347
+00:27:11,680 --> 00:27:15,400
+بدنا نعرف انه انا ال dilution اش هو ال dilution ال
+
+348
+00:27:15,400 --> 00:27:22,840
+dilution سامبل على سامبل
+
+349
+00:27:22,840 --> 00:27:27,660
+ال dilution
+
+350
+00:27:33,960 --> 00:27:37,860
+التخفيف ال dilution شو هو ال dilution ال dilution
+
+351
+00:27:37,860 --> 00:27:42,180
+هو ال volume of sample على ال volume of sample
+
+352
+00:27:42,180 --> 00:27:46,780
+زائد ال volume of diluent طبعا طيب ال volume of
+
+353
+00:27:46,780 --> 00:27:50,220
+هذا هو بالنسبة لدى ال dilution طب ال dilution
+
+354
+00:27:50,220 --> 00:27:55,800
+بيعطينى اللى هو واحد على عشرة يبقى هان واحد على
+
+355
+00:27:55,800 --> 00:28:05,100
+عشرة واحد على عشرة طيب هلأالـ sample هي هتكون قداش
+
+356
+00:28:05,100 --> 00:28:11,400
+الـ sample هي اللي هو او التخفيف اللي هو واحد على
+
+357
+00:28:11,400 --> 00:28:16,600
+واحد على عشرة هذا هو ال total يبقى ال total هو
+
+358
+00:28:16,600 --> 00:28:21,680
+العشرة ال sample زائد ال diluent هو ال total اللي
+
+359
+00:28:21,680 --> 00:28:26,120
+هو عشرة من هذا ممكن اني انا اقدر قداش اجيب ال
+
+360
+00:28:26,120 --> 00:28:31,220
+volume of sample يبقى ال total هيساوي
+
+361
+00:28:32,840 --> 00:28:39,520
+اللي هو ال sample زائد ال diluent ال total بساوي
+
+362
+00:28:39,520 --> 00:28:46,000
+ال sample زائد ال diluent ويساوي عشرة
+
+363
+00:28:46,000 --> 00:28:50,340
+من الدنيا اللي هو من خلال ال dilution واحد على
+
+364
+00:28:50,340 --> 00:28:54,540
+عشرة يبقى ال total هو عشرة طب قاللي ان ال diluent
+
+365
+00:28:54,540 --> 00:28:58,580
+في ال tube أربعة و نص يبقى ال diluent موجودة هنا
+
+366
+00:28:58,580 --> 00:29:04,780
+انعوض في القانون هناالـ sample مش معروفة أنا بدي
+
+367
+00:29:04,780 --> 00:29:13,460
+أجيبها زائد ال diluent أربعة و نص و ال total من ال
+
+368
+00:29:13,460 --> 00:29:17,020
+dilution جيبناها عشر طب قداش ال sample اللي هي ال
+
+369
+00:29:17,020 --> 00:29:22,640
+sample هتساوي نص يبقى
+
+370
+00:29:22,640 --> 00:29:26,240
+ال sample هتكون نص هيك أنا جيبت ال sample ممكن ما
+
+371
+00:29:26,240 --> 00:29:31,060
+يكون مدينيها في القانون أو في المسألة فانا بدي
+
+372
+00:29:31,060 --> 00:29:34,880
+أجيب ال sampleنفس الفكرة ممكن يجيب لل sample لكن
+
+373
+00:29:34,880 --> 00:29:38,280
+مايجيب لل diluent يقول لل sample اللي أنا أخدتها
+
+374
+00:29:38,280 --> 00:29:42,580
+من أول tube اللي هي نص ML ال dilution واحد من عشرة
+
+375
+00:29:42,580 --> 00:29:46,660
+قداش ال volume of diluent هعوض بنفس القانون sample
+
+376
+00:29:46,660 --> 00:29:50,680
+على sample زائد diluent واحد على عشرة التعطل اللي
+
+377
+00:29:50,680 --> 00:29:53,700
+هي ال sample زائد ال diluent عشرة ال sample
+
+378
+00:29:53,700 --> 00:29:57,440
+قدانيها اللي هي نص زائد ال diluent مش مدينيها
+
+379
+00:29:57,440 --> 00:30:02,640
+ويساوي عشرة هحسب بالنهاية هيطلع ال diluent نصهك
+
+380
+00:30:02,640 --> 00:30:07,700
+أنا ممكن يبقى في المسألة مايجيبليش الـ Diluent
+
+381
+00:30:07,700 --> 00:30:10,560
+ممكن مايجيبليش الـ Dilution ممكن مايجيبليش الـ
+
+382
+00:30:10,560 --> 00:30:15,960
+Volume of Sample كلهم بنفس الفكرة من خلال القانون
+
+383
+00:30:15,960 --> 00:30:19,840
+Sample على Sample زائد Diluent هو هذا الـ Dilution
+
+384
+00:30:19,840 --> 00:30:23,820
+من خلاله أنا بقدر أجيب أي واحد منهم وأكمل المسألة
+
+385
+00:30:23,820 --> 00:30:28,920
+وأحلها بشكل بسيط وسهلقلنا ممكن العينة الأصلية
+
+386
+00:30:28,920 --> 00:30:32,640
+مباشرة أخد منها فبالتالي مش هخفف ال tube أو ال
+
+387
+00:30:32,640 --> 00:30:37,370
+tubeلو العينة الأصلية مخففة زي ما ذكرنا في مثال
+
+388
+00:30:37,370 --> 00:30:40,810
+اللي كان مكتوب فيه عشر أقصار بواحد تخفيفها لازم
+
+389
+00:30:40,810 --> 00:30:44,590
+أنا أضرب أول tube في تخفيف هذه العينة ممكن مايست
+
+390
+00:30:44,590 --> 00:30:49,490
+كل التخفيف يقولي والله أنا أخدت 25 مل مع 225
+
+391
+00:30:49,490 --> 00:30:53,110
+ديليوانت فوقتها أنا لازم أحسب ال dilution من خلال
+
+392
+00:30:53,110 --> 00:30:56,290
+هذا القانون سابل على سامل زائد دوليوانت هي الدين
+
+393
+00:30:56,290 --> 00:31:00,450
+ال dilution هحسب ال dilution وبعدين أضرب في هذا ال
+
+394
+00:31:00,450 --> 00:31:04,540
+dilution في تخفيف أول tubeهذه كل الأفكار اللي ممكن
+
+395
+00:31:04,540 --> 00:31:10,100
+تيجي بالنسبة لمسائل الـ Serial Delusion نرجع
+
+396
+00:31:10,100 --> 00:31:16,200
+للمثال اللي في الملزمة أو في الـ Slides فهذا
+
+397
+00:31:16,200 --> 00:31:20,300
+المثال ذاكر أنه أخد 1ML من العينة يبقى هذه العينة
+
+398
+00:31:20,300 --> 00:31:26,600
+مش مخففة تمام في كل tube حط 9MLNormal saline اللي
+
+399
+00:31:26,600 --> 00:31:30,020
+هو الـ Diluent أخد واحد مئل من هايلت يوب يبقى
+
+400
+00:31:30,020 --> 00:31:33,680
+تخفيفها واحد على واحد زائد تسعة واحد اللي هي ال
+
+401
+00:31:33,680 --> 00:31:36,820
+sample على واحد ال sample زائد ال diluent تسعة
+
+402
+00:31:36,820 --> 00:31:41,540
+يبقى واحد على عشرة عشرة أسالب واحد وقلنا هاي مش
+
+403
+00:31:41,540 --> 00:31:45,790
+مخففة فبالتالي مش هضرفيه أي تخفيفتخفيف التيوب
+
+404
+00:31:45,790 --> 00:31:49,430
+التاني أخدت 1 ML من التيوب الأول على التيوب التاني
+
+405
+00:31:49,430 --> 00:31:54,290
+يبقى التخفيف 1 على 1 زائد 9 يبقى 1 على 10 في تخفيف
+
+406
+00:31:54,290 --> 00:31:57,890
+التيوب القابل 10 أقل سالب 1 يبقى 10 أقل سالب 2
+
+407
+00:31:57,890 --> 00:32:05,650
+وهكذا هكمل في كل تيوب ماخد من كل تيوب 1 ML تمام
+
+408
+00:32:05,650 --> 00:32:09,470
+أخد 1 ML من كل تيوب وزرعوا على إبلات قلنا هنلاحظ
+
+409
+00:32:09,470 --> 00:32:13,310
+أن أحنا في أول تيوبات هتكون two numerous to count
+
+410
+00:32:13,310 --> 00:32:18,500
+وآخر تيوب twoif you to count يبقى هدولة التانية
+
+411
+00:32:18,500 --> 00:32:21,800
+كانوا two many colonies to count مش هعود عنهم في
+
+412
+00:32:21,800 --> 00:32:28,180
+القانون هدول أقل من تلاتين تلت كولوني أخر تلت
+
+413
+00:32:28,180 --> 00:32:32,780
+تخفيفات أقل من تلتين فبالتالي هقول two if you to
+
+414
+00:32:32,780 --> 00:32:36,600
+count هذا ما بين التلتين والتلتمية فهو اللي هعوده
+
+415
+00:32:36,600 --> 00:32:40,040
+في القانون اللي هو مية و تسعة و خمسين كولوني كان
+
+416
+00:32:40,040 --> 00:32:43,700
+تخفيفه عشرة والسالب تلاتة هنعوده في القانون اللي
+
+417
+00:32:43,700 --> 00:32:50,160
+هومية مية و تسعة و خمسين على أس ال volume اللي أنا
+
+418
+00:32:50,160 --> 00:32:56,880
+أخدته اللي هو واحد على عشرة أسالب تلاتة طبعا عشرة
+
+419
+00:32:56,880 --> 00:33:00,520
+أسالب تلاتة بتطلع على فوق فبالتالي بتصير عشرة اف
+
+420
+00:33:00,520 --> 00:33:04,100
+تلاتة بتطلع بالنهاية القانون كله farming units per
+
+421
+00:33:04,100 --> 00:33:08,520
+milliliter لأنه كانت سائلة فبالتالي per milliliter
+
+422
+00:33:08,520 --> 00:33:12,960
+هاي هذا مثالبقولي هان روحتي إنتي عدتي لقولي في
+
+423
+00:33:12,960 --> 00:33:18,880
+الطبخ كان 46 بما أنه كان 46 يبقى بحطه في القانون
+
+424
+00:33:18,880 --> 00:33:22,740
+تمام ما بين ال 30 وال 300 يبقى أنا وقتها هان هعود
+
+425
+00:33:22,740 --> 00:33:25,940
+في القانون قال لي حطيتي 1 ml من ال bacterial
+
+426
+00:33:25,940 --> 00:33:31,170
+culture ل 99 ml من ال salineوأخدت من هذا الـ tube
+
+427
+00:33:31,170 --> 00:33:34,590
+واحد من عشرة ML وروحتي زرعتي يبقى الـ dilution
+
+428
+00:33:34,590 --> 00:33:38,530
+هيكون واحد على واحد زائد تسعة وتسعين اللي هي واحد
+
+429
+00:33:38,530 --> 00:33:42,430
+على مية يبقى هاي التخفيف واحد على مية الكل هو ستة
+
+430
+00:33:42,430 --> 00:33:46,470
+و أربعين القس اللي هي واحد من عشرة ML يبقى بعود في
+
+431
+00:33:46,470 --> 00:33:50,950
+القانون ستة و أربعين ضرب مية ليش لأنه ال dilution
+
+432
+00:33:50,950 --> 00:33:55,090
+أنا طلعته لفوق كان عشرة أس سالب اتنين طلعته لفوق
+
+433
+00:33:55,090 --> 00:33:59,860
+صار عشرة أس اتنين اللي هي في المية ضربالواحد على
+
+434
+00:33:59,860 --> 00:34:06,000
+واحد من عشرة الواحد من عشرة طلعت فا يبقى سارت عشرة
+
+435
+00:34:06,000 --> 00:34:11,340
+فهيك القانون بالنهاية ستة واربعين الف colony
+
+436
+00:34:11,340 --> 00:34:17,960
+farming unit per ml هذا بالنسبة لهذا
+
+437
+00:34:17,960 --> 00:34:22,360
+المثال هيك ما نكون خلصنا معمل ال cereal dilution
+
+438
+00:34:22,360 --> 00:34:27,810
+methodبيجي طبعا أسئلة مثال حسابات أحسبي اللي هو ال
+
+439
+00:34:27,810 --> 00:34:32,730
+colony farming unit في بير ميلي later في هذا ال في
+
+440
+00:34:32,730 --> 00:34:36,150
+هاي العينة وقولنا الأسئلة ممكن يكون ال dilution مش
+
+441
+00:34:36,150 --> 00:34:42,010
+موجود ممكن ال volume of blood مش موجود ممكنممكن ال
+
+442
+00:34:42,010 --> 00:34:46,550
+volume of sample أنت تحسبيه ممكن ال daily weight
+
+443
+00:34:46,550 --> 00:34:49,870
+أنت تحسبيه ممكن العينة تكون مش مخففة ممكن تكون
+
+444
+00:34:49,870 --> 00:34:53,670
+مخففة فهي كل الأمور بدنا ننتبه عليها برضه ممكن
+
+445
+00:34:53,670 --> 00:34:58,170
+يجيبلك عدد الكولوني أقل من تلاتين أو أكتر من
+
+446
+00:34:58,170 --> 00:35:02,170
+تلاتمية فبالتالي هانش هلجأ للتعويض في القانون هكتب
+
+447
+00:35:02,170 --> 00:35:07,410
+two few to count or two numerous to countهك مينكون
+
+448
+00:35:07,410 --> 00:35:11,510
+خلصنا الشعب تر يعطيكم العافية والسلام عليكم ورحمة
+
+449
+00:35:11,510 --> 00:35:12,570
+الله وبركاته
+
diff --git a/PL9fwy3NUQKwZWgAb_V1ATlRRsH-6i3cPC/kghd40S327U_raw.json b/PL9fwy3NUQKwZWgAb_V1ATlRRsH-6i3cPC/kghd40S327U_raw.json
new file mode 100644
index 0000000000000000000000000000000000000000..5c4ec304916f04a8935d3ef007cf264cefdd1672
--- /dev/null
+++ b/PL9fwy3NUQKwZWgAb_V1ATlRRsH-6i3cPC/kghd40S327U_raw.json
@@ -0,0 +1 @@
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by extrapolation this number can in turn be used to calculate the number of colony forming unit in the original sample طبعا انا بعد عدد بعد الكولوني وبنهاية بعبر عن اللى اللى هي الوحدة بكولوني forming unit في هذا", "tokens": [3615, 16254, 2655, 11296, 8978, 23758, 5296, 36150, 9307, 13672, 7578, 25961, 13672, 7578, 1975, 8315, 1975, 8315, 1975, 9381, 15040, 30767, 2288, 34268, 3224, 9154, 23758, 294, 300, 2107, 11504, 1448, 9154, 23758, 16712, 9778, 5172, 33911, 538, 48224, 399, 341, 1230, 393, 294, 1261, 312, 1143, 281, 8873, 264, 1230, 295, 23028, 15745, 4985, 294, 264, 3380, 6889, 23032, 3555, 3615, 995, 1975, 8315, 45030, 3215, 6225, 3215, 3215, 45030, 3215, 33251, 12610, 11536, 1829, 46599, 1863, 11296, 10632, 45030, 26890, 18871, 13672, 7578, 13672, 7578, 39896, 2423, 2407, 24401, 3660, 4724, 4117, 12610, 11536, 1829, 15745, 4985, 8978, 23758], "avg_logprob": -0.22906553600598306, "compression_ratio": 1.7489878542510122, "no_speech_prob": 5.9604644775390625e-06, "words": 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count by this method are usually expressed as colony forming unit per millimeter بقوللي عدد البكتيريا في هذه الطريقة بعبّر عنها ب colony forming unit", "tokens": [259, 264, 3380, 6889, 39182, 19446, 5551, 22488, 6055, 9778, 5172, 33911, 4724, 11242, 25513, 8978, 13672, 1829, 31439, 6055, 9778, 5172, 33911, 18863, 9957, 3660, 11331, 49975, 5551, 5016, 36520, 15844, 6225, 3215, 3215, 29739, 4117, 2655, 13546, 25528, 8978, 18863, 9957, 3660, 16247, 36520, 10632, 35632, 1207, 538, 341, 3170, 366, 2673, 12675, 382, 23028, 15745, 4985, 680, 17942, 4724, 39648, 20292, 6225, 3215, 3215, 29739, 4117, 2655, 13546, 25528, 8978, 29538, 41950, 16572, 28671, 45030, 3555, 11703, 2288, 18871, 11296, 4724, 23028, 15745, 4985], "avg_logprob": -0.1896306767382405, "compression_ratio": 1.6218487394957983, "no_speech_prob": 1.621246337890625e-05, "words": [{"start": 625.01, "end": 625.25, "word": "in", "probability": 0.1533203125}, {"start": 625.25, "end": 625.43, "word": " the", "probability": 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{"start": 631.19, "end": 631.45, "word": " حتى", "probability": 0.925048828125}, {"start": 631.45, "end": 631.93, "word": " أحصل", "probability": 0.9381510416666666}, {"start": 631.93, "end": 632.29, "word": " على", "probability": 0.900390625}, {"start": 632.29, "end": 633.27, "word": " عدد", "probability": 0.9886067708333334}, {"start": 633.27, "end": 634.27, "word": " البكتيريا", "probability": 0.921875}, {"start": 634.27, "end": 634.57, "word": " في", "probability": 0.9794921875}, {"start": 634.57, "end": 635.29, "word": " العينة", "probability": 0.9246419270833334}, {"start": 635.29, "end": 636.29, "word": " الأصلية", "probability": 0.8011881510416666}, {"start": 636.29, "end": 637.19, "word": " bacterial", "probability": 0.775390625}, {"start": 637.19, "end": 637.95, "word": " count", "probability": 0.908203125}, {"start": 637.95, "end": 638.63, "word": " by", "probability": 0.943359375}, {"start": 638.63, "end": 639.01, "word": " this", "probability": 0.67333984375}, {"start": 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او اجرام حسب نوع العينة", "tokens": [610, 23271, 1975, 2407, 1975, 7435, 2288, 10943, 11331, 35457, 8717, 45367, 18863, 9957, 3660, 1975, 15730, 25961, 2655, 8608, 16373, 37977, 45030, 26890, 4724, 1829, 9673, 20292, 5296, 36081, 2288, 4032, 45636, 995, 25961, 2655, 20328, 46152, 3660, 45030, 26890, 4724, 1829, 5296, 7435, 2288, 10943, 7251, 3555, 4587, 7578, 28242, 3224, 9452, 299, 4985, 680, 23271, 1975, 2407, 1975, 7435, 2288, 10943, 11331, 35457, 8717, 45367, 18863, 9957, 3660], "avg_logprob": -0.2191840304682652, "compression_ratio": 1.7737226277372262, "no_speech_prob": 8.046627044677734e-06, "words": [{"start": 651.37, "end": 651.77, "word": "per", "probability": 0.472412109375}, {"start": 651.77, "end": 652.27, "word": " ml", "probability": 0.80126953125}, {"start": 652.27, "end": 652.79, "word": " او", "probability": 0.78076171875}, {"start": 652.79, "end": 653.27, "word": " اجرام", "probability": 0.901611328125}, {"start": 653.27, "end": 653.79, "word": " حسب", "probability": 0.963623046875}, {"start": 653.79, "end": 654.41, "word": " نوع", "probability": 0.98291015625}, {"start": 654.41, "end": 654.97, "word": " العينة", "probability": 0.7875162760416666}, {"start": 654.97, "end": 655.17, "word": " اذا", "probability": 0.74755859375}, {"start": 655.17, "end": 655.57, "word": " كانت", "probability": 0.987548828125}, {"start": 655.57, "end": 655.97, "word": " سائلة", "probability": 0.8743489583333334}, {"start": 655.97, "end": 656.41, "word": " بعبر", "probability": 0.5869140625}, {"start": 656.41, "end": 656.81, "word": " بي", "probability": 0.4658203125}, {"start": 656.81, "end": 657.39, "word": " الملي", "probability": 0.653076171875}, {"start": 657.39, "end": 658.71, "word": " ليتر", "probability": 0.7389322916666666}, {"start": 658.71, "end": 659.47, "word": " واذا", "probability": 0.7661946614583334}, {"start": 659.47, "end": 659.93, "word": " كانت", "probability": 0.986572265625}, {"start": 659.93, "end": 660.35, "word": " صلبة", "probability": 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27981, 322, 11504, 1448, 5924, 4099, 1296, 39182, 19446, 1975, 8315, 1975, 25957, 1211, 33345, 399, 5296, 16247, 9566, 3555, 995, 4587, 13672, 1829, 1975, 8315], "avg_logprob": -0.2184495163651613, "compression_ratio": 1.5310077519379846, "no_speech_prob": 3.0100345611572266e-05, "words": [{"start": 670.09, "end": 670.69, "word": "Normally", "probability": 0.6069234212239584}, {"start": 670.69, "end": 670.93, "word": " the", "probability": 0.51318359375}, {"start": 670.93, "end": 671.43, "word": " bacterial", "probability": 0.81005859375}, {"start": 671.43, "end": 672.01, "word": " sample", "probability": 0.79052734375}, {"start": 672.01, "end": 672.33, "word": " is", "probability": 0.92822265625}, {"start": 672.33, "end": 672.87, "word": " diluted", "probability": 0.973388671875}, {"start": 672.87, "end": 673.41, "word": " by", "probability": 0.96142578125}, {"start": 673.41, "end": 674.07, "word": " factor", "probability": 0.56298828125}, {"start": 674.07, "end": 674.55, "word": " of", "probability": 0.63134765625}, {"start": 674.55, "end": 674.95, "word": " 10", "probability": 0.6875}, {"start": 674.95, "end": 675.25, "word": " and", "probability": 0.87548828125}, {"start": 675.25, "end": 675.69, "word": " plated", "probability": 0.506866455078125}, {"start": 675.69, "end": 675.93, "word": " on", "probability": 0.9189453125}, {"start": 675.93, "end": 676.25, "word": " agar", "probability": 0.88720703125}, {"start": 676.25, "end": 676.77, "word": " بقوللي", "probability": 0.6083170572916666}, {"start": 676.77, "end": 678.43, "word": " عادة", "probability": 0.9801432291666666}, {"start": 678.43, "end": 678.91, "word": " احنا", "probability": 0.9051106770833334}, {"start": 678.91, "end": 679.31, "word": " لما", "probability": 0.970458984375}, {"start": 679.31, "end": 679.53, "word": " انا", "probability": 0.923828125}, {"start": 679.53, "end": 680.13, "word": " اعمل", "probability": 0.9544270833333334}, {"start": 680.13, "end": 680.85, "word": " تخفيف", "probability": 0.939453125}, {"start": 680.85, "end": 682.05, "word": " لعينة", "probability": 0.7003173828125}, {"start": 682.05, "end": 683.13, "word": " الاصلية", "probability": 0.79656982421875}, {"start": 683.13, "end": 683.49, "word": " بروح", "probability": 0.9627278645833334}, {"start": 683.49, "end": 683.89, "word": " بعمل", "probability": 0.9666341145833334}, {"start": 683.89, "end": 684.83, "word": " بمضاعفات", "probability": 0.96923828125}, {"start": 684.83, "end": 685.49, "word": " العشرة", "probability": 0.9300130208333334}, {"start": 685.49, "end": 686.33, "word": " و", "probability": 0.67529296875}, {"start": 686.33, "end": 687.21, "word": " بزراعها", "probability": 0.87841796875}, {"start": 687.21, "end": 687.45, "word": " على", "probability": 0.89453125}, {"start": 687.45, "end": 688.05, "word": " الagar", "probability": 0.75537109375}, {"start": 688.05, "end": 689.01, "word": " after", "probability": 0.62744140625}, {"start": 689.01, "end": 689.87, "word": " incubation", "probability": 0.97021484375}, {"start": 689.87, "end": 690.23, "word": " the", "probability": 0.732421875}, {"start": 690.23, "end": 690.69, "word": " number", "probability": 0.9228515625}, {"start": 690.69, "end": 690.97, "word": " of", "probability": 0.9736328125}, {"start": 690.97, "end": 691.47, "word": " colonies", "probability": 0.92919921875}, {"start": 691.47, "end": 691.91, "word": " on", "probability": 0.93115234375}, {"start": 691.91, "end": 692.53, "word": " dilution", "probability": 0.960205078125}, {"start": 692.53, "end": 692.87, "word": " plate", "probability": 0.6767578125}, {"start": 692.87, "end": 693.29, "word": " showing", "probability": 0.74072265625}, {"start": 693.29, "end": 693.93, "word": " between", "probability": 0.9501953125}, {"start": 693.93, "end": 696.09, "word": " بعد", "probability": 0.8779296875}, {"start": 696.09, "end": 696.31, "word": " ما", "probability": 0.90771484375}, {"start": 696.31, "end": 696.51, "word": " انا", 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118709, "start": 1166.48, "end": 1187.1, "text": "من high ل tube وضفتها على ال diluent وحسبت ال dilution بدون ما اضرب ب dilution ل هذه العينة لأن هذه العينة مش مخففة يبقى ننتبه في السؤال هل العينة الأصلية مخففة إذا مخففة لازم أضرب أول ال tube في تخفيف high ل tube العينة الأصلية", "tokens": [27842, 1090, 5296, 9917, 4032, 11242, 5172, 2655, 11296, 15844, 2423, 11504, 19226, 4032, 5016, 35457, 2655, 2423, 11504, 1448, 47525, 11536, 19446, 1975, 11242, 25513, 4724, 11504, 1448, 5296, 29538, 18863, 9957, 3660, 5296, 33456, 29538, 18863, 9957, 3660, 37893, 3714, 9778, 5172, 5172, 3660, 7251, 3555, 4587, 7578, 8717, 29399, 3555, 3224, 8978, 21136, 33604, 6027, 8032, 1211, 18863, 9957, 3660, 16247, 36520, 10632, 3714, 9778, 5172, 5172, 3660, 11933, 15730, 3714, 9778, 5172, 5172, 3660, 5296, 31377, 2304, 5551, 11242, 25513, 5551, 12610, 2423, 9917, 8978, 6055, 9778, 5172, 33911, 1090, 5296, 9917, 18863, 9957, 3660, 16247, 36520, 10632], "avg_logprob": -0.17217839747956656, "compression_ratio": 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2107.01, "word": "هك", "probability": 0.846435546875}, {"start": 2107.01, "end": 2107.41, "word": " مينكون", "probability": 0.5128580729166666}, {"start": 2107.41, "end": 2108.03, "word": " خلصنا", "probability": 0.98486328125}, {"start": 2108.03, "end": 2108.93, "word": " الشعب", "probability": 0.6597493489583334}, {"start": 2108.93, "end": 2109.15, "word": " تر", "probability": 0.6942138671875}, {"start": 2109.15, "end": 2109.61, "word": " يعطيكم", "probability": 0.82451171875}, {"start": 2109.61, "end": 2110.15, "word": " العافية", "probability": 0.9583333333333334}, {"start": 2110.15, "end": 2110.65, "word": " والسلام", "probability": 0.9392903645833334}, {"start": 2110.65, "end": 2111.11, "word": " عليكم", "probability": 0.9365234375}, {"start": 2111.11, "end": 2111.51, "word": " ورحمة", "probability": 0.9581298828125}, {"start": 2111.51, "end": 2111.81, "word": " الله", "probability": 0.97314453125}, {"start": 2111.81, "end": 2112.57, "word": " وبركاته", "probability": 0.965625}], "temperature": 1.0}], "language": "ar", "language_probability": 1.0, "duration": 2113.387375, "duration_after_vad": 2044.4331249999946}
\ No newline at end of file
diff --git a/PL9fwy3NUQKwZWgAb_V1ATlRRsH-6i3cPC/kghd40S327U_raw.srt b/PL9fwy3NUQKwZWgAb_V1ATlRRsH-6i3cPC/kghd40S327U_raw.srt
new file mode 100644
index 0000000000000000000000000000000000000000..cb27db09cac567f061017b4ab3fb055f4b6a2d05
--- /dev/null
+++ b/PL9fwy3NUQKwZWgAb_V1ATlRRsH-6i3cPC/kghd40S327U_raw.srt
@@ -0,0 +1,1796 @@
+1
+00:00:02,530 --> 00:00:06,450
+السلام عليكم ورحمة الله وبركاته يعطيكم العافية
+
+2
+00:00:06,450 --> 00:00:10,410
+جميعا في هذا اللقاء هتكون المحاضرة بعنوان الـ
+
+3
+00:00:10,410 --> 00:00:13,790
+Serial Dilution Method of Bacteria Enumeration
+
+4
+00:00:13,790 --> 00:00:18,870
+طريقة عد البكتيريا باستخدام الـ Serial Dilution
+
+5
+00:00:18,870 --> 00:00:23,710
+Method طبعا هنذكر أكثر من طريقة لعد البكتيريا لكن
+
+6
+00:00:23,710 --> 00:00:27,550
+في هذا المعمل هنشرح طريقة الـ Serial Dilution
+
+7
+00:00:27,550 --> 00:00:31,490
+Method many studies require the quantitative
+
+8
+00:00:32,150 --> 00:00:35,470
+determination of bacterial population واللي في
+
+9
+00:00:35,470 --> 00:00:39,950
+كثير من الدراسات بتحتاج ان انا اعد البكتيريا
+
+10
+00:00:39,950 --> 00:00:43,410
+quantitative determination of bacterial population
+
+11
+00:00:43,410 --> 00:00:48,410
+quantitative يعني عد البكتيريا لكن لما انا احكي
+
+12
+00:00:48,410 --> 00:00:53,170
+qualitative qualitative يعني presence or absence
+
+13
+00:00:53,170 --> 00:00:56,790
+بدي اشوف هل البكتيريا موجودة ولا لأ لكن
+
+14
+00:00:56,790 --> 00:01:01,340
+quantitative بتكلم على عد البكتيريابقول في عندي
+
+15
+00:01:01,340 --> 00:01:05,540
+طريقتين لعد البكتيريا the two most widely used
+
+16
+00:01:05,540 --> 00:01:10,240
+method for determining bacterial numbers are
+
+17
+00:01:10,240 --> 00:01:14,540
+الطريقتين اللي أنا بستخدمهم في عد البكتيريا ال
+
+18
+00:01:14,540 --> 00:01:18,560
+most common أول طريقة اللي هي ال standard blood
+
+19
+00:01:18,560 --> 00:01:22,820
+count method اللي هنتكلم عنها في هذا المعمل اللي
+
+20
+00:01:22,820 --> 00:01:27,300
+هي cereal dilution method الطريقة التانية ال
+
+21
+00:01:27,300 --> 00:01:31,880
+spectrophotometerالـ Terbidiometric Analysis
+
+22
+00:01:31,880 --> 00:01:37,040
+بتعتمد على الـ Terbidity طبعا هذه الطريقة هتذكرها
+
+23
+00:01:37,040 --> 00:01:41,800
+في ال chapter اللي معنوه في chapter اللي هو ال
+
+24
+00:01:41,800 --> 00:01:44,040
+bacterial growth اللي هي طريقة الـ
+
+25
+00:01:44,040 --> 00:01:49,160
+Spectrophotometer بقول لي طريقة ال standard blood
+
+26
+00:01:49,160 --> 00:01:53,440
+count method أو Serial dilution method is an
+
+27
+00:01:53,440 --> 00:01:57,840
+indirect measurement of cell density هي طريقة
+
+28
+00:01:57,840 --> 00:01:58,620
+indirect
+
+29
+00:02:10,430 --> 00:02:14,650
+indirect measurement ليش قاللي طريقة غير مباشرة
+
+30
+00:02:14,650 --> 00:02:21,310
+لأنه في التعبير على عدد البكتيريا بكتب في نهاية
+
+31
+00:02:21,310 --> 00:02:28,070
+الحل colony farming unit الوحدة اللي أنا بعبر عنها
+
+32
+00:02:28,070 --> 00:02:33,150
+هي colony farming unit per ml أو اجرام حسب العينة
+
+33
+00:02:33,530 --> 00:02:37,430
+طيب كولوني يعني مستعمرة المستعمرة انا ما بعرف قداش
+
+34
+00:02:37,430 --> 00:02:40,930
+البكتيريا الموجودة فيها ممكن تكون single ممكن تكون
+
+35
+00:02:40,930 --> 00:02:45,570
+chain فبالتالي انا بعبر ب colony farming unit عشان
+
+36
+00:02:45,570 --> 00:02:48,890
+هيك انا بسمي هذه الطريقة او بحكي عن هذه الطريقة
+
+37
+00:02:48,890 --> 00:02:54,310
+indirect measurement طيب ليش قاللي ان هي فقط بتقيس
+
+38
+00:02:54,310 --> 00:02:58,800
+ال live bacteria بتقيس البكتيريا الحيةمنعد في ال
+
+39
+00:02:58,800 --> 00:03:02,080
+standard blood count method فقط البكتيريا الحية
+
+40
+00:03:02,080 --> 00:03:06,280
+لأنه لو ميتة أكيد مش هتتكثر لأنه بعتمد على ال
+
+41
+00:03:06,280 --> 00:03:10,620
+blood count method ال blood count method ان انا
+
+42
+00:03:10,620 --> 00:03:16,960
+بدي أزرع او أعمل تخفيف للعينة بعدين أزرع كل تخفيف
+
+43
+00:03:16,960 --> 00:03:21,800
+على طبق و أحطها في ال incubator فبالتالي البكتيريا
+
+44
+00:03:21,800 --> 00:03:26,850
+لو ميتة مش هتتكثرفي هذا الطبق عشان هيك فقط في هذه
+
+45
+00:03:26,850 --> 00:03:30,990
+الطريقة بتقيس البكتيريا أو من عد البكتيريا الحية
+
+46
+00:03:30,990 --> 00:03:36,690
+طيب الطريقة التانية لـ spectrophotometer analysis
+
+47
+00:03:36,690 --> 00:03:40,130
+بقول بتعتمد على الـ turbidity عشان هيك اسمها
+
+48
+00:03:40,130 --> 00:03:46,110
+turbidometric analysis or spectrophotometer بقول
+
+49
+00:03:46,110 --> 00:03:50,760
+بتعتمد على ال turbidityindirectly measured برضه
+
+50
+00:03:50,760 --> 00:03:54,060
+نفس ال standard blood count هي تعتبر indirect
+
+51
+00:03:54,060 --> 00:03:58,420
+measurement لماذا؟ لأنها بتعتمد على ال turbidity
+
+52
+00:03:58,420 --> 00:04:03,200
+طبعا العكارة ما بقدر اميز البكتيريا الحية
+
+53
+00:04:03,200 --> 00:04:06,520
+والبكتيريا الميتة لما يكون في انمو على شكل ال
+
+54
+00:04:06,520 --> 00:04:10,180
+growth على شكل العكارة على شكل ال turbidity ما
+
+55
+00:04:10,180 --> 00:04:14,810
+بقدر اميزهل البكتيريا حية ولا ميتة البكتيريا سواء
+
+56
+00:04:14,810 --> 00:04:18,390
+كانت حية او ميتة بيكون في عندي turbidity بيكون في
+
+57
+00:04:18,390 --> 00:04:23,630
+عندي عكارة تمام يبقى سواء كانت طبعا هي تحت ال
+
+58
+00:04:23,630 --> 00:04:27,190
+microscope البكتيريا او في عندي عكارة مابقدر اميز
+
+59
+00:04:27,190 --> 00:04:30,450
+بين البكتيريا الحية والميتة لكن على الطبق فقط
+
+60
+00:04:30,450 --> 00:04:35,070
+البكتيريا الحية هي اللي بتتكثر يبقى بطريقة ال
+
+61
+00:04:35,070 --> 00:04:38,940
+standard blood count انا بعد البكتيريا الحيةفي
+
+62
+00:04:38,940 --> 00:04:43,900
+طريقة الـ Spectrophotometer بقيس البكتيريا الحية
+
+63
+00:04:43,900 --> 00:04:50,000
+والميتة تمام هذا بالنسبة للطريقتين الطريقتين طبعا
+
+64
+00:04:50,000 --> 00:04:54,540
+هم العد البكتيريا الـ Quantitative طب نيجي لأول
+
+65
+00:04:54,540 --> 00:04:59,380
+طريقة اللى هتكون او اللى هنشرح عنها في هذا المعمل
+
+66
+00:04:59,380 --> 00:05:03,440
+اللى هى ال standard blood count method اللى بسميها
+
+67
+00:05:03,440 --> 00:05:04,820
+viable count
+
+68
+00:05:09,180 --> 00:05:13,840
+the blood count viable count يعني حية لأن هي بتعد
+
+69
+00:05:13,840 --> 00:05:16,760
+البكتيريا الحية يبقى إلها اسم تاني اللي هي ال
+
+70
+00:05:16,760 --> 00:05:21,680
+viable count لأنه بتعد فقط البكتيريا الحية the
+
+71
+00:05:21,680 --> 00:05:25,500
+number of bacteria in a given sample is usually
+
+72
+00:05:25,500 --> 00:05:30,440
+too great to be counted directly بقولي البكتيريا
+
+73
+00:05:30,440 --> 00:05:35,300
+أو عدد البكتيريا في عينة معينة بيكون كتير كبير
+
+74
+00:05:35,300 --> 00:05:39,970
+لإني أنا أقدر أعدهافبالتالي لو انت روحتي اخدتي من
+
+75
+00:05:39,970 --> 00:05:44,650
+العينة الاصلية روحتي زلعتيها على الطبق هيكون كل
+
+76
+00:05:44,650 --> 00:05:48,890
+الطبق مليان مستعمرات بكتيريا فبالتالي انا مش هقدر
+
+77
+00:05:48,890 --> 00:05:54,510
+اعد مش هعرف انا اعد هاي ال colony عشان هيك بنلجأ
+
+78
+00:05:54,510 --> 00:05:58,850
+لطريقة ال dilution however if the sample is
+
+79
+00:05:58,850 --> 00:06:03,110
+serially diluted بقولي ان العينة انا بعملي لها
+
+80
+00:06:03,110 --> 00:06:08,040
+تخفيف عشان اني انا اقدر اعدهاتمام لازم احنا نخفف
+
+81
+00:06:08,040 --> 00:06:12,080
+العينة الأصلية فبالتالي بيكون العدد قليل بقدر اني
+
+82
+00:06:12,080 --> 00:06:17,080
+انا عيدها بعدها بدرب في ال factor اللي انا اللي هو
+
+83
+00:06:17,080 --> 00:06:23,280
+شو التخفيف شو ال dilution بدرب فيه ال factor and
+
+84
+00:06:23,280 --> 00:06:27,740
+then blotted out on an agar surface in such manner
+
+85
+00:06:27,740 --> 00:06:31,020
+that single isolated bacteria from visible
+
+86
+00:06:31,020 --> 00:06:35,460
+isolated colonyبقولي بعد ما تعملي serial dilution
+
+87
+00:06:35,460 --> 00:06:40,700
+تعملي تخفيف للعينة الأصلية بروح أنا باخد من كل
+
+88
+00:06:40,700 --> 00:06:47,200
+تخفيف حجم معين تقريبا كنا ناخد اللي هو واحد من
+
+89
+00:06:47,200 --> 00:06:52,720
+عشرة ML من كل تخفيفه أروح أنا أزرعه على طبق تمام
+
+90
+00:06:52,720 --> 00:06:57,980
+بعدين أفرد هذه الحجم الواحد من عشرة ML أفرده على
+
+91
+00:06:57,980 --> 00:07:03,380
+الطبق باستخدام الالشيبممكن كنا نعمل من كل إت يوب
+
+92
+00:07:03,380 --> 00:07:08,000
+نعمل two بلات طبقين و بعد بقى أنا أعيد أخد ال
+
+93
+00:07:08,000 --> 00:07:11,320
+average بينهم ممكن أعمل على طبق واحد أزرع كل إت
+
+94
+00:07:11,320 --> 00:07:14,780
+يوب كل تخفيف على طبق واحد أو ممكن على طبقين و
+
+95
+00:07:14,780 --> 00:07:19,140
+بعدين أخد ال average بنفرضها بالالشف و بنحطها في
+
+96
+00:07:19,140 --> 00:07:23,240
+ال incubator و بعد 24 ساعة بشوف ال count بشوف
+
+97
+00:07:23,240 --> 00:07:29,030
+العدربقولي كل single isolated bacteria بتكون من
+
+98
+00:07:29,030 --> 00:07:32,870
+isolated colony بتعبر على ال isolated colony the
+
+99
+00:07:32,870 --> 00:07:36,430
+number of colony can be used as a measure of the
+
+100
+00:07:36,430 --> 00:07:40,050
+number of viable living cell in the non-dilution
+
+101
+00:07:40,050 --> 00:07:44,730
+بقولي عدد ال colony اللي أنا بدي أشوفها بعد ما
+
+102
+00:07:44,730 --> 00:07:51,190
+أحطها في ال incubator هتعبر عنعدد الخلايا الحية في
+
+103
+00:07:51,190 --> 00:07:55,010
+هذا الـ dilution في هذا التخفيف اللي أنا أخدت منه
+
+104
+00:07:55,010 --> 00:08:02,830
+وزرعته على الطبق تمام هذا بالنسبة لأول slide نيجي
+
+105
+00:08:02,830 --> 00:08:06,610
+keep in mind that if the organism normally forms
+
+106
+00:08:06,610 --> 00:08:10,210
+multiple cell arrangements such as chain the
+
+107
+00:08:10,210 --> 00:08:13,830
+colony farming unit may consist of a chain of
+
+108
+00:08:13,830 --> 00:08:18,030
+bacteria rather than a single bacteria بقوللي انه
+
+109
+00:08:18,350 --> 00:08:21,810
+أحنا قلنا في أول معمل لما تعرفنا على الشاب قلنا
+
+110
+00:08:21,810 --> 00:08:25,870
+البكتيريا ممكن تكون في عندي كمان arrangement ترتيب
+
+111
+00:08:25,870 --> 00:08:30,950
+للخلايا البكتيرية حسب مستوى الانقصام ممكن
+
+112
+00:08:30,950 --> 00:08:34,870
+البكتيريا تكون على اتشان على شكل سلسلة ممكن تكون
+
+113
+00:08:34,870 --> 00:08:39,830
+على شكل تجمعات تمام فبالتالي لما أنا أعبر بال
+
+114
+00:08:39,830 --> 00:08:43,710
+colony farming unit ال colony farming unitقلنا
+
+115
+00:08:43,710 --> 00:08:47,790
+ممكن انها تكون الكوني تجمع من الخلايا البكتيرية ال
+
+116
+00:08:47,790 --> 00:08:51,190
+single cell بقولي هان مش برضه مش ممكن انها تكون
+
+117
+00:08:51,190 --> 00:08:55,570
+على شكل single بكتيريا ممكن انها تكون chain of
+
+118
+00:08:55,570 --> 00:09:00,910
+بكتيريا فبالتالي هان انا بعتبر ال measurement
+
+119
+00:09:00,910 --> 00:09:05,300
+indirect بقولي in additionsome of the bacteria may
+
+120
+00:09:05,300 --> 00:09:09,120
+be clumped together برضه ممكن البكتيريا تتجمع مع
+
+121
+00:09:09,120 --> 00:09:12,820
+بعضها زي ما قلنا ممكن انها تكون على شكل أسطاف على
+
+122
+00:09:12,820 --> 00:09:17,480
+شكل انقود العنب فبالتالي therefore we doing the
+
+123
+00:09:17,480 --> 00:09:21,720
+blood cow technique we generally say we are
+
+124
+00:09:21,720 --> 00:09:24,800
+determining the number of colony farming unit in
+
+125
+00:09:24,800 --> 00:09:28,600
+that non dilution عشان هيك انا لما اعمل طريقة ال
+
+126
+00:09:28,600 --> 00:09:33,810
+cereal dilution or blood cow techniqueبعبّر
+
+127
+00:09:33,810 --> 00:09:38,390
+بالنهاية على العدد بـ Colony Farming Unit ما بحكي
+
+128
+00:09:38,390 --> 00:09:41,610
+Single Bacteria او Each and Bacteria لأنه بالنهاية
+
+129
+00:09:41,610 --> 00:09:46,270
+المستعمرة ممكن تكون عبارة عن Single Bacterium ممكن
+
+130
+00:09:46,270 --> 00:09:50,390
+تكون Each and ممكن تكون Clump فبعبّر على Colony
+
+131
+00:09:50,390 --> 00:09:54,770
+Farming Unit اللي هو المستعمرة عدد المستعمرات اللي
+
+132
+00:09:54,770 --> 00:10:00,480
+أناعديتها في هذا لبلات اللى كان اللى انا انا اصلا
+
+133
+00:10:00,480 --> 00:10:04,780
+زرعته من هذا in that non dilution من هذا التخفيف
+
+134
+00:10:04,780 --> 00:10:10,300
+by extrapolation this number can in turn be used
+
+135
+00:10:10,300 --> 00:10:13,420
+to calculate the number of colony forming unit in
+
+136
+00:10:13,420 --> 00:10:18,120
+the original sample طبعا انا بعد عدد بعد الكولوني
+
+137
+00:10:18,120 --> 00:10:23,000
+وبنهاية بعبر عن اللى اللى هي الوحدة بكولوني
+
+138
+00:10:23,000 --> 00:10:27,690
+forming unit في هذاin the original sample بعد ما
+
+139
+00:10:27,690 --> 00:10:31,930
+أعد تخفيف بضرب في اللي هو تخفيف العينة حتى أحصل
+
+140
+00:10:31,930 --> 00:10:37,190
+على عدد البكتيريا في العينة الأصلية bacterial
+
+141
+00:10:37,190 --> 00:10:42,570
+count by this method are usually expressed as
+
+142
+00:10:42,570 --> 00:10:46,490
+colony forming unit per millimeter بقوللي عدد
+
+143
+00:10:46,490 --> 00:10:49,930
+البكتيريا في هذه الطريقة بعبّر عنها ب colony
+
+144
+00:10:49,930 --> 00:10:55,170
+forming unitper ml او اجرام حسب نوع العينة اذا
+
+145
+00:10:55,170 --> 00:11:00,850
+كانت سائلة بعبر بي الملي ليتر واذا كانت صلبة بعبر
+
+146
+00:11:00,850 --> 00:11:04,950
+بي لجرام يبقى كله uniformic unit per ml او اجرام
+
+147
+00:11:04,950 --> 00:11:06,370
+حسب نوع العينة
+
+148
+00:11:10,090 --> 00:11:14,070
+Normally the bacterial sample is diluted by factor
+
+149
+00:11:14,070 --> 00:11:19,530
+of 10 and plated on agar بقوللي عادة احنا لما انا
+
+150
+00:11:19,530 --> 00:11:25,490
+اعمل تخفيف لعينة الاصلية بروح بعمل بمضاعفات العشرة
+
+151
+00:11:25,490 --> 00:11:30,690
+و بزراعها على الagar after incubation the number
+
+152
+00:11:30,690 --> 00:11:36,090
+of colonies on dilution plate showing between بعد
+
+153
+00:11:36,090 --> 00:11:40,560
+ما انا اعمل incubation ل الأطباق اللي اناأخدت من
+
+154
+00:11:40,560 --> 00:11:46,500
+كل تخفيف حجم معين وروحت زرعته على قبلة طبق او
+
+155
+00:11:46,500 --> 00:11:50,920
+طبقين من كل تخفيف بعد ال incubation من عد هاي ال
+
+156
+00:11:50,920 --> 00:11:55,800
+colony مين الأطباق اللي انا بدي اعدها لو كان فقط
+
+157
+00:11:55,800 --> 00:12:01,380
+كان عدد ال colony ما بين 30 ل 300 colony هو هذا
+
+158
+00:12:01,380 --> 00:12:06,700
+اللي انا بدي اعوضه بالنهاية في القانونهى بقولى
+
+159
+00:12:06,700 --> 00:12:10,900
+البلاد اللى فى بين تلاتين لتلاتمية كولونى هو اللى
+
+160
+00:12:10,900 --> 00:12:16,600
+أنا بختاره لإن هو ال significant هو اللى إله أهمية
+
+161
+00:12:16,600 --> 00:12:22,660
+طيب لو كان عدد الكولونى أقل من تلاتين كولونى فى
+
+162
+00:12:22,660 --> 00:12:28,060
+الطبق بقولى تكتبي too few to count على طول من حد
+
+163
+00:12:28,060 --> 00:12:31,420
+ما تشوفى عدد الكولونى فى الطبق أقل من تلاتين
+
+164
+00:12:31,420 --> 00:12:36,570
+مابعود فى القانون بكتب tooto few to count طيب لو
+
+165
+00:12:36,570 --> 00:12:43,610
+كان اكتر من تلاتمية ما بعده بكتب فيه ال result to
+
+166
+00:12:43,610 --> 00:12:47,630
+numerous to count يبقى لو كان اقل من تلاتين to few
+
+167
+00:12:47,630 --> 00:12:51,650
+to count اللي هو اختصار هايم او اذا كان اكتر من
+
+168
+00:12:51,650 --> 00:12:59,070
+تلاتمية بكتب to numerous to count تمام؟لكن لو كان
+
+169
+00:12:59,070 --> 00:13:03,130
+بين الـ 30 والـ 300 بعد هاي الكلوني وبالنهاية بعود
+
+170
+00:13:03,130 --> 00:13:07,290
+بالقانون اللي هندكره في ال slides الجاي هذا البلاد
+
+171
+00:13:07,290 --> 00:13:12,790
+تقريبا عندي انا اللي هو 7 او 8 كلوني فبالتالي هو
+
+172
+00:13:12,790 --> 00:13:17,670
+اقل من 30 فبكتب على طول too few to count هو مش
+
+173
+00:13:17,670 --> 00:13:21,530
+مناسب اني انا اعدهم هم نلاحظ ان هو تقريبا ما بين
+
+174
+00:13:21,530 --> 00:13:25,750
+ال 30 لل 300 فبعد هاي الكلوني قداش عددها وبالنهاية
+
+175
+00:13:26,200 --> 00:13:29,680
+بعود ال number of colony فيه قانون هنتعرف عليه
+
+176
+00:13:29,680 --> 00:13:33,580
+نلاحظ ان هذا الطبق كتير اكتر من 300 تقريبا
+
+177
+00:13:33,580 --> 00:13:37,440
+فبالتالي صعب اني انا اعده فعلى طول ما بعد بكتب 2
+
+178
+00:13:37,440 --> 00:13:42,240
+in numerous to count طيب
+
+179
+00:13:42,240 --> 00:13:48,480
+نيجي للخطوات بالنسبة للخطوات كيف انا بدي اعمل
+
+180
+00:13:48,480 --> 00:13:51,920
+serial dilution method او blood count method او
+
+181
+00:13:51,920 --> 00:13:56,460
+viable countبجيب العينة الأصلية اللي أنا بدي أشوف
+
+182
+00:13:56,460 --> 00:14:00,500
+عدد البكتيريا في هذه العينة وقلنا بدنا نعمل serial
+
+183
+00:14:00,500 --> 00:14:05,700
+dilution هلأ ممكن أنا هذه العينة أخد منها مباشرة
+
+184
+00:14:05,700 --> 00:14:09,540
+وممكن أني أنا أخففها في هذا المثال ماخد منها
+
+185
+00:14:09,540 --> 00:14:16,160
+مباشرة ماخد واحد MLوعمل طبعا التيوبات اللي هي لأ
+
+186
+00:14:16,160 --> 00:14:20,100
+التخفيف طبعا أنا بحط diluent في هذه التيوبات تمام
+
+187
+00:14:20,100 --> 00:14:24,160
+على الأغلب بحط 9 ml normal saline 9 ml normal
+
+188
+00:14:24,160 --> 00:14:28,940
+saline 9 ml normal saline ممكن عادي أستخدم مثلا 4
+
+189
+00:14:28,940 --> 00:14:34,140
+.5 ml normal saline المهم بالنهاية يكون التخفيف
+
+190
+00:14:34,140 --> 00:14:39,140
+أنا عارفة شو التخفيف اللي أنا هضرب فيهكان حاطة 9
+
+191
+00:14:39,140 --> 00:14:44,400
+ml normal saline أخد 1 ml من العينة وضافها على أول
+
+192
+00:14:44,400 --> 00:14:47,920
+tube اللي هو اللي فيه daily one 9 ml normal saline
+
+193
+00:14:47,920 --> 00:14:53,960
+طيب شو هو التخفيف في هذه ال tube التخفيف اللي هو
+
+194
+00:14:53,960 --> 00:14:58,160
+ال sample على ال sample زائد ال daily one أنا أخدت
+
+195
+00:14:58,160 --> 00:15:02,640
+1 ml يبقى ال sample 1 ml على ال sample زائد ال
+
+196
+00:15:02,640 --> 00:15:06,040
+daily one ال sample 1 زائد ال daily one 9 يبقى 1
+
+197
+00:15:06,040 --> 00:15:11,020
+على 10يبقى التخفيف عشرة أس سالب واحد في هذه ال
+
+198
+00:15:11,020 --> 00:15:15,440
+tube طيب نيجي لتخفيف العينة التانية بروح أنا باخد
+
+199
+00:15:15,440 --> 00:15:19,580
+من العينة الأولى واحد ML و بحطها في هذه ال tube
+
+200
+00:15:19,580 --> 00:15:23,840
+تخفيف هذه ال tube واحد طبعا اللي هو ال sample على
+
+201
+00:15:23,840 --> 00:15:27,240
+ال total واحد زائد تسعة يبقى واحد على عشرة عشرة أس
+
+202
+00:15:27,240 --> 00:15:31,960
+سالب واحد درب تخفيف العينة اللي قبلها اللي هي عشرة
+
+203
+00:15:31,960 --> 00:15:36,910
+أس سالب واحد يبقى عشرة أس سالب اتنيننفس الفكرة هان
+
+204
+00:15:36,910 --> 00:15:40,670
+هاخد 1ml من هايلي تيوب و اضيفها على لي تيوب التالت
+
+205
+00:15:40,670 --> 00:15:45,030
+يبقى تخفيف هايلي تيوب اللي هي واحد على واحد زائد
+
+206
+00:15:45,030 --> 00:15:48,570
+تسعة ال sample اللي انا اخدت واحد ml من هان على
+
+207
+00:15:48,570 --> 00:15:53,270
+واحد زائد تسعة الدوليوان يبقى واحد على عشرة عشرة
+
+208
+00:15:53,270 --> 00:15:58,930
+أسالب واحد هو التخفيف هايلي تيوب ضرب تخفيفالتيوب
+
+209
+00:15:58,930 --> 00:16:01,510
+اللي قبلها اللي هو كان عشرة أقصالب اتنين يبقى عشرة
+
+210
+00:16:01,510 --> 00:16:04,730
+أقصالب تلاتة بنكمل بنفس الفكرة بيطلع في هاي التيوب
+
+211
+00:16:04,730 --> 00:16:09,170
+عشرة أقصالب أربعة عشرة أقصالب خمسة عشرة أقصالب ستة
+
+212
+00:16:09,170 --> 00:16:14,810
+هيك عرفنا تخفيف كل تيوب بعد ما أعمل تخفيف في هاي
+
+213
+00:16:14,810 --> 00:16:19,490
+التيوب لازم أكون عارف أنا أو كاتبة على التيوب أنه
+
+214
+00:16:19,490 --> 00:16:23,310
+هاي والله تخفيفها عشرة أقصالب واحد هاي عشرة أقصالب
+
+215
+00:16:23,310 --> 00:16:26,780
+اتنين وعلى الطبق اللي أنا هزرع منه نفس الفكرةهكتب
+
+216
+00:16:26,780 --> 00:16:30,200
+هاي تخفيفها عشرة أقصالب واحد هاي عشرة أقصالب اتنين
+
+217
+00:16:30,200 --> 00:16:33,440
+عشرة أقصالب تلاتة عشرة أقصالب أربعة عشرة أقصالب
+
+218
+00:16:33,440 --> 00:16:37,240
+خمسة وعشرة أقصالب ستة بعد ما أعمل تخفيف
+
+219
+00:16:40,700 --> 00:16:48,580
+هو مش ذاكر المهم هاخد من كل التيوب حجم معين لازم
+
+220
+00:16:48,580 --> 00:16:52,160
+انا اكون عارفة هذا الحجم لأن بعد ما اعود هعود فيه
+
+221
+00:16:52,160 --> 00:16:56,220
+القانون على سبيل المثال هاخد 1 من 10 ML من كل
+
+222
+00:16:56,220 --> 00:16:59,920
+التيوب وهزرعه على الطبق على طبق معين ممكن قلنا
+
+223
+00:16:59,920 --> 00:17:03,820
+نعمل نزرع على كل التيوب على طبقين واخد الـAverage
+
+224
+00:17:03,820 --> 00:17:08,580
+المتوسط ناخد على سبيل المثال 1 من 10 ML من هذا
+
+225
+00:17:08,580 --> 00:17:14,090
+التيوبو بحطه على إبلات و بزرع و بفرد و هكذا في كل
+
+226
+00:17:14,090 --> 00:17:19,910
+تيوبات بعد 24 ساعة بعمل count ل .. ال .. ال ..
+
+227
+00:17:19,910 --> 00:17:26,010
+اللي هو ال .. الإبلات لكلنا طبعا هتطلع في عندي هان
+
+228
+00:17:26,010 --> 00:17:30,610
+في الأغلب أقل تخفيف اللي هي too numerous to count
+
+229
+00:17:30,610 --> 00:17:34,630
+أخر إشي هتكون too few to count لكن هان تقريبا
+
+230
+00:17:34,630 --> 00:17:37,670
+هيطلع في ال .. اللي في النص ممكن إن أنا أقدر أعدها
+
+231
+00:17:38,150 --> 00:17:42,570
+فهعيد كل كله نزل و اعوض في القانون اللى هنذكره في
+
+232
+00:17:42,570 --> 00:17:46,070
+ال slides اللى بعدي انا بقول انك انت بتنقلي طبعا
+
+233
+00:17:46,070 --> 00:17:49,590
+واحد ML من كل tube بتنقليها لل tube التانى و
+
+234
+00:17:49,590 --> 00:17:52,890
+بالاها النهاية التخفيف هو تخفيفها في تخفيف اللى
+
+235
+00:17:52,890 --> 00:17:59,490
+قبلها نفس الفكرة بعدين بتعملي طبعا بتاخدي من كل
+
+236
+00:17:59,490 --> 00:18:04,250
+tube و بتحطيه على طبق أجار مكتوب و بعدين بتعملي
+
+237
+00:18:04,250 --> 00:18:10,040
+اللى هو incubationمن 24 ل 48 ساعة بعدين بقولي
+
+238
+00:18:10,040 --> 00:18:13,080
+بتهدي ال colony في الأطباق فقط لما يكون عدد ال
+
+239
+00:18:13,080 --> 00:18:19,620
+colony من 30 ل 200 أو ل 300 colonies لو كان two
+
+240
+00:18:19,620 --> 00:18:23,820
+numerous to count أكتر من 300 بكتب two numerous to
+
+241
+00:18:23,820 --> 00:18:28,240
+count لو كان أقل من 30 بكتب two few to count هذا
+
+242
+00:18:28,240 --> 00:18:33,580
+بالنسبة للطريقة اللي أنا بشتغل عليها في هاي ال
+
+243
+00:18:33,580 --> 00:18:39,940
+slide نفس الفكرةلكن الاختلاف انه هانذاكر في ليتيوب
+
+244
+00:18:39,940 --> 00:18:43,580
+مش حاطة 9 ML احنا قولنا مش شرط اني انا احط 9 ML
+
+245
+00:18:43,580 --> 00:18:49,560
+بحط اي اي حجم بديا لكن بالنهاية لازم اكون عارف هذا
+
+246
+00:18:49,560 --> 00:18:54,640
+الحجم على اللي على اساسه هحسب ال dilution هانحطت 4
+
+247
+00:18:54,640 --> 00:18:59,860
+.5 ML من ال diluent واخد نص ML من اللي هو ليتيوب
+
+248
+00:18:59,860 --> 00:19:04,340
+يبقى نص كيف دي احسب dilution هاي ليتيوب نص علىنص
+
+249
+00:19:04,340 --> 00:19:08,700
+زائد أربعة ونص بيطلع بالنهاية اللي هو عشرة أس سالب
+
+250
+00:19:08,700 --> 00:19:13,200
+واحد طيب ليش هانحطت عشرة أس سالب اتنين تخفيفها لأن
+
+251
+00:19:13,200 --> 00:19:21,540
+هاي العينة مخففة في المثال اللي قبل في
+
+252
+00:19:21,540 --> 00:19:25,500
+المثال هدا كانت العينة الأصلية مش مخففة أخدت واحد
+
+253
+00:19:25,500 --> 00:19:30,960
+MLمن high ل tube وضفتها على ال diluent وحسبت ال
+
+254
+00:19:30,960 --> 00:19:34,480
+dilution بدون ما اضرب ب dilution ل هذه العينة لأن
+
+255
+00:19:34,480 --> 00:19:39,980
+هذه العينة مش مخففة يبقى ننتبه في السؤال هل العينة
+
+256
+00:19:39,980 --> 00:19:44,340
+الأصلية مخففة إذا مخففة لازم أضرب أول ال tube في
+
+257
+00:19:44,340 --> 00:19:48,930
+تخفيف high ل tube العينة الأصليةلكن لو مش مخففة
+
+258
+00:19:48,930 --> 00:19:53,590
+خلاص على طول زى ما ذاكر في هذا المثال على طول بعمل
+
+259
+00:19:53,590 --> 00:19:57,070
+اللى هو التخفيف بحسب التخفيف بدون ما اضرب في
+
+260
+00:19:57,070 --> 00:20:00,350
+dilution high لـ tube لأنه high لـ tube مش مخففة
+
+261
+00:20:00,350 --> 00:20:07,010
+فهذا المثال هان العينة high مخففة وذا كل تخفيفها
+
+262
+00:20:07,010 --> 00:20:12,310
+عشرة أس تالب واحد يبقى تخفيف أول tube اللى هو ال
+
+263
+00:20:12,310 --> 00:20:15,890
+sample اللى أنا أخدته نص على ال sample نص زائد
+
+264
+00:20:15,890 --> 00:20:21,110
+أربعة و نص يبقىبنهاية بطلع نص على خمسة نص على خمسة
+
+265
+00:20:21,110 --> 00:20:25,650
+واحد على عشر عشرة أسالب واحد في تخفيف ال tube اللي
+
+266
+00:20:25,650 --> 00:20:28,330
+قبلها اللي هي عشرة أسالب واحد يبقى عشرة أسالب
+
+267
+00:20:28,330 --> 00:20:32,470
+اتنين طبعا هاي ال tube تخفيفها أربع بعمل بنفس
+
+268
+00:20:32,470 --> 00:20:36,350
+الفكرة نص على نص زائد أربع ونص عشرة أسالب واحد درب
+
+269
+00:20:36,350 --> 00:20:40,230
+تخفيف هاي عشرة أسالب اتنين بنهاية بطلع عشرة أسالب
+
+270
+00:20:40,230 --> 00:20:42,530
+أربع في هاي ال tube عشرة أسالب خمسة في هاي ال tube
+
+271
+00:20:42,530 --> 00:20:48,160
+عشرة أسالب ستة في هاي ال tubeأخد من كل الـ tube نص
+
+272
+00:20:48,160 --> 00:20:53,640
+أو واحد من عشرة ML و راح زرعها في طبق تمام راح
+
+273
+00:20:53,640 --> 00:20:59,060
+زرعها في هاي الأطباق و حطها في ال incubator يبقى
+
+274
+00:20:59,060 --> 00:21:03,400
+في هذا المثال ينتبه أنه هان العينة هاي مخففة فلازم
+
+275
+00:21:03,400 --> 00:21:06,860
+أضرب أول tube في تخفيفها لكن في المثال اللي ان ذكر
+
+276
+00:21:06,860 --> 00:21:11,540
+في الأول ماكانت العينة مخففة عشان هيك ماضربتش ممكن
+
+277
+00:21:11,540 --> 00:21:16,320
+في مثال تاني ما يذكرلياللي هو هان تخفيفها عشر أقل
+
+278
+00:21:16,320 --> 00:21:23,120
+سالب واحد ممكن يقولي والله أخدت خمسة وعشرين ML من
+
+279
+00:21:23,120 --> 00:21:27,740
+الملك وضفتهم على مائتين وخمسة وعشرين من ال normal
+
+280
+00:21:27,740 --> 00:21:32,060
+saline فهان بيدي وقتها أحسب تخفيف اللي هو هاي ال
+
+281
+00:21:32,060 --> 00:21:36,000
+sample اللي بنحكي خمسة وعشرين على خمسة وعشرين زائد
+
+282
+00:21:36,000 --> 00:21:38,620
+مائتين وخمسة وعشرين اللي هو ال sample زائد ال do
+
+283
+00:21:38,620 --> 00:21:43,860
+you want فبطلع خمسة وعشرين على مائتين وخمسين25 على
+
+284
+00:21:43,860 --> 00:21:48,340
+250 اللي هي 1 على 10 اللي هي 10 أساليب واحد هيك
+
+285
+00:21:48,340 --> 00:21:54,040
+بكون حاسبة تخفيف هاي لتيوب و بكمل حل زي ما كملنا
+
+286
+00:21:54,040 --> 00:21:58,960
+يبقى ممكن ما يكون مخفف العينة ممكن يكون مخففها و
+
+287
+00:21:58,960 --> 00:22:04,200
+يذكرلك التخفيف ممكن يكون فيه تخفيف لكن مش ذاكر شو
+
+288
+00:22:04,200 --> 00:22:07,620
+قيمة ال dilution فانت بدك تحسب قيمة ال dilution من
+
+289
+00:22:07,620 --> 00:22:13,400
+القانون sample على sample زائد diluentهذا بالنسبة
+
+290
+00:22:13,400 --> 00:22:18,700
+لهذه الصورة هذه السؤال هذا الفكرة نفس اللي احنا
+
+291
+00:22:18,700 --> 00:22:22,060
+ذكرناها في الأول أخد واحد ML وضفنا قولنا كل واحد
+
+292
+00:22:22,060 --> 00:22:25,180
+قداشر ال dilution هي ذكرهم عشر أقل سالب واحد عشر
+
+293
+00:22:25,180 --> 00:22:27,860
+أقل سالب اتنين عشر أقل سالب تلات عشر أقل سالب أربع
+
+294
+00:22:27,860 --> 00:22:33,400
+عشر أقل سالب خمسة عشر أقل سالب ستةواحد من عشر ML
+
+295
+00:22:33,400 --> 00:22:42,840
+منها يليت يوبات وراح زرعهم في اللي هو الاطباق
+
+296
+00:22:42,840 --> 00:22:51,120
+وبعد 24 ساعة شاف ال colonialism طيب
+
+297
+00:22:51,120 --> 00:22:56,430
+احنا قلنا بعد 24 ساعةبحسب او بعد ال colony اذا كان
+
+298
+00:22:56,430 --> 00:22:59,990
+لبلاد ما بين تلاتين لتلاتمية colony farming unit
+
+299
+00:22:59,990 --> 00:23:03,390
+ومنعود بالقانون شهو هو القانون اللى بنعود عليه
+
+300
+00:23:03,390 --> 00:23:07,010
+بقول لي calculate the number of bacteria colony
+
+301
+00:23:07,010 --> 00:23:11,890
+farming unit per milliliter or gram of sample by
+
+302
+00:23:11,890 --> 00:23:14,930
+dividing the number of colonies كيف بدي اعد او بدي
+
+303
+00:23:14,930 --> 00:23:18,690
+احسب احنا قولنا بالنهاية لبلاد count بعبر عنها ب
+
+304
+00:23:18,690 --> 00:23:23,940
+colony farming unit per ml or gramتمام طيب كيف
+
+305
+00:23:23,940 --> 00:23:27,620
+بالنهاية بدي أحسب هاي ال .. ال measures أو كيف بدي
+
+306
+00:23:27,620 --> 00:23:31,780
+أعبر بـ Colony Farming Unit per ML Origram بقولي
+
+307
+00:23:31,780 --> 00:23:35,980
+بتقسمي ال number of colonies عدد ال colony اللي
+
+308
+00:23:35,980 --> 00:23:40,820
+أنتي عدتيه على الطبق اللي كان ما بين 30 ل 300
+
+309
+00:23:40,820 --> 00:23:44,820
+قولنا ما بعود في القانون إذا كان أقل من 30 و إذا
+
+310
+00:23:44,820 --> 00:23:52,430
+أكتر من 300بعد كده بحط على الـ Sample شو هذا الـ S
+
+311
+00:23:52,430 --> 00:23:57,030
+رمز الـ S بقول لي هو كمية العينة اللي إنتي أخدتيها
+
+312
+00:23:57,030 --> 00:24:02,810
+من الـ YouTube يعني في هذا الـ YouTube أنا روحت
+
+313
+00:24:02,810 --> 00:24:06,750
+أخدت من هذا الـ YouTube واحد من عشرة ML وحطيته في
+
+314
+00:24:06,750 --> 00:24:10,130
+البلاد وروحت فردته وزرعت قداش أنا أخدت من الـ
+
+315
+00:24:10,130 --> 00:24:17,340
+YouTube دا عوض يبقى هان واحد من عشرة هي الـ Sواحد
+
+316
+00:24:17,340 --> 00:24:20,380
+من عشراء يبقى ال number of colony عدد ال colony
+
+317
+00:24:20,380 --> 00:24:24,980
+اللي عديتها في الطبق الأساس هو أداش أنا أخدت من
+
+318
+00:24:24,980 --> 00:24:30,140
+التخفيف و روحت زراعته على الطبق ال دي اللي هي ال
+
+319
+00:24:30,140 --> 00:24:34,020
+dilution شو ال tube اللي أنا أخدت منه أداش كان ال
+
+320
+00:24:34,020 --> 00:24:37,540
+dilution أداش كان التخفيف في هذه ال tube أيو
+
+321
+00:24:37,540 --> 00:24:41,340
+number of colonies دي dilution blankالـ S هي
+
+322
+00:24:41,340 --> 00:24:45,280
+volume transferred to blood قداش حجم العينة اللي
+
+323
+00:24:45,280 --> 00:24:48,980
+أنا أخدتها من ال tube في هذا ال dilution وحطيته
+
+324
+00:24:48,980 --> 00:24:51,860
+على ال blood بالنهاية هعبّر على كل uniform units
+
+325
+00:24:51,860 --> 00:25:00,360
+per ml or اجرام طيب قبل ما نذكر هذا المثال في
+
+326
+00:25:00,360 --> 00:25:07,450
+الخطوات في الخطواتفي هذا المثال ذاكر مثلا أنه أخد
+
+327
+00:25:07,450 --> 00:25:13,750
+1ML من العينة وحطينا 9ML من الـ diluent طيب ممكن
+
+328
+00:25:13,750 --> 00:25:22,270
+ما يقوللي أنه قداش أنا مثلا اللي هو قداش أنا أخدت
+
+329
+00:25:22,270 --> 00:25:26,650
+الـ diluent ممكن ما يكون ذاكر قداش رقم الـ diluent
+
+330
+00:25:26,650 --> 00:25:30,690
+قداش اللي هو 9ML هاي مش ذاكرها أو مش ذاكر ال 1ML
+
+331
+00:25:31,540 --> 00:25:37,280
+عشان نروح يقولي ال dilution في لتيوب هاي واحد على
+
+332
+00:25:37,280 --> 00:25:43,440
+عشرة تمام ال dilution في هاي لتيوب واحد على عشرة
+
+333
+00:25:43,440 --> 00:25:49,800
+وفي هاي لتيوب ضفتي انتي اربع و نص diluent قداش ال
+
+334
+00:25:49,800 --> 00:25:54,640
+volume of sample اللي أخدتي يبقى ممكن يسألي سؤال
+
+335
+00:25:54,640 --> 00:25:59,810
+كيف نلعب الأسئلةهان يقول لي ان في اليوتيوب انا
+
+336
+00:25:59,810 --> 00:26:07,110
+أخدت 4.5 ML ديليوانت والتخفيف 1 على 10 او 10 أسالب
+
+337
+00:26:07,110 --> 00:26:11,730
+1 بقول لي ال volume of sample قداش كيف بدي أحسب ال
+
+338
+00:26:11,730 --> 00:26:16,450
+volume of sample يعني اللي هي هان هادي قداش هي مش
+
+339
+00:26:16,450 --> 00:26:21,030
+ذاكرها نيجي عشان تكون الصورة واضحة اللي هو ال
+
+340
+00:26:21,030 --> 00:26:21,390
+diligent
+
+341
+00:26:33,560 --> 00:26:37,800
+in a tube اربعة
+
+342
+00:26:37,800 --> 00:26:41,920
+و نص ML
+
+343
+00:26:41,920 --> 00:26:50,800
+ال dilution اللي
+
+344
+00:26:50,800 --> 00:26:56,480
+هو واحد على بقولي
+
+345
+00:26:56,480 --> 00:27:02,080
+ال volume of
+
+346
+00:27:03,240 --> 00:27:11,680
+سامبل انت بدك تجيبى قداش هو كيف انا بدى اجيبه هلأ
+
+347
+00:27:11,680 --> 00:27:15,400
+بدنا نعرف انه انا ال dilution اش هو ال dilution ال
+
+348
+00:27:15,400 --> 00:27:22,840
+dilution سامبل على سامبل
+
+349
+00:27:22,840 --> 00:27:27,660
+ال dilution
+
+350
+00:27:33,960 --> 00:27:37,860
+التخفيف ال dilution شو هو ال dilution ال dilution
+
+351
+00:27:37,860 --> 00:27:42,180
+هو ال volume of sample على ال volume of sample
+
+352
+00:27:42,180 --> 00:27:46,780
+زائد ال volume of diluent طبعا طيب ال volume of
+
+353
+00:27:46,780 --> 00:27:50,220
+هذا هو بالنسبة لدى ال dilution طب ال dilution
+
+354
+00:27:50,220 --> 00:27:55,800
+بيعطينى اللى هو واحد على عشرة يبقى هان واحد على
+
+355
+00:27:55,800 --> 00:28:05,100
+عشرة واحد على عشرة طيب هلأالـ sample هي هتكون قداش
+
+356
+00:28:05,100 --> 00:28:11,400
+الـ sample هي اللي هو او التخفيف اللي هو واحد على
+
+357
+00:28:11,400 --> 00:28:16,600
+واحد على عشرة هذا هو ال total يبقى ال total هو
+
+358
+00:28:16,600 --> 00:28:21,680
+العشرة ال sample زائد ال diluent هو ال total اللي
+
+359
+00:28:21,680 --> 00:28:26,120
+هو عشرة من هذا ممكن اني انا اقدر قداش اجيب ال
+
+360
+00:28:26,120 --> 00:28:31,220
+volume of sample يبقى ال total هيساوي
+
+361
+00:28:32,840 --> 00:28:39,520
+اللي هو ال sample زائد ال diluent ال total بساوي
+
+362
+00:28:39,520 --> 00:28:46,000
+ال sample زائد ال diluent ويساوي عشرة
+
+363
+00:28:46,000 --> 00:28:50,340
+من الدنيا اللي هو من خلال ال dilution واحد على
+
+364
+00:28:50,340 --> 00:28:54,540
+عشرة يبقى ال total هو عشرة طب قاللي ان ال diluent
+
+365
+00:28:54,540 --> 00:28:58,580
+في ال tube أربعة و نص يبقى ال diluent موجودة هنا
+
+366
+00:28:58,580 --> 00:29:04,780
+انعوض في القانون هناالـ sample مش معروفة أنا بدي
+
+367
+00:29:04,780 --> 00:29:13,460
+أجيبها زائد ال diluent أربعة و نص و ال total من ال
+
+368
+00:29:13,460 --> 00:29:17,020
+dilution جيبناها عشر طب قداش ال sample اللي هي ال
+
+369
+00:29:17,020 --> 00:29:22,640
+sample هتساوي نص يبقى
+
+370
+00:29:22,640 --> 00:29:26,240
+ال sample هتكون نص هيك أنا جيبت ال sample ممكن ما
+
+371
+00:29:26,240 --> 00:29:31,060
+يكون مدينيها في القانون أو في المسألة فانا بدي
+
+372
+00:29:31,060 --> 00:29:34,880
+أجيب ال sampleنفس الفكرة ممكن يجيب لل sample لكن
+
+373
+00:29:34,880 --> 00:29:38,280
+مايجيب لل diluent يقول لل sample اللي أنا أخدتها
+
+374
+00:29:38,280 --> 00:29:42,580
+من أول tube اللي هي نص ML ال dilution واحد من عشرة
+
+375
+00:29:42,580 --> 00:29:46,660
+قداش ال volume of diluent هعوض بنفس القانون sample
+
+376
+00:29:46,660 --> 00:29:50,680
+على sample زائد diluent واحد على عشرة التعطل اللي
+
+377
+00:29:50,680 --> 00:29:53,700
+هي ال sample زائد ال diluent عشرة ال sample
+
+378
+00:29:53,700 --> 00:29:57,440
+قدانيها اللي هي نص زائد ال diluent مش مدينيها
+
+379
+00:29:57,440 --> 00:30:02,640
+ويساوي عشرة هحسب بالنهاية هيطلع ال diluent نصهك
+
+380
+00:30:02,640 --> 00:30:07,700
+أنا ممكن يبقى في المسألة مايجيبليش الـ Diluent
+
+381
+00:30:07,700 --> 00:30:10,560
+ممكن مايجيبليش الـ Dilution ممكن مايجيبليش الـ
+
+382
+00:30:10,560 --> 00:30:15,960
+Volume of Sample كلهم بنفس الفكرة من خلال القانون
+
+383
+00:30:15,960 --> 00:30:19,840
+Sample على Sample زائد Diluent هو هذا الـ Dilution
+
+384
+00:30:19,840 --> 00:30:23,820
+من خلاله أنا بقدر أجيب أي واحد منهم وأكمل المسألة
+
+385
+00:30:23,820 --> 00:30:28,920
+وأحلها بشكل بسيط وسهلقلنا ممكن العينة الأصلية
+
+386
+00:30:28,920 --> 00:30:32,640
+مباشرة أخد منها فبالتالي مش هخفف ال tube أو ال
+
+387
+00:30:32,640 --> 00:30:37,370
+tubeلو العينة الأصلية مخففة زي ما ذكرنا في مثال
+
+388
+00:30:37,370 --> 00:30:40,810
+اللي كان مكتوب فيه عشر أقصار بواحد تخفيفها لازم
+
+389
+00:30:40,810 --> 00:30:44,590
+أنا أضرب أول tube في تخفيف هذه العينة ممكن مايست
+
+390
+00:30:44,590 --> 00:30:49,490
+كل التخفيف يقولي والله أنا أخدت 25 مل مع 225
+
+391
+00:30:49,490 --> 00:30:53,110
+ديليوانت فوقتها أنا لازم أحسب ال dilution من خلال
+
+392
+00:30:53,110 --> 00:30:56,290
+هذا القانون سابل على سامل زائد دوليوانت هي الدين
+
+393
+00:30:56,290 --> 00:31:00,450
+ال dilution هحسب ال dilution وبعدين أضرب في هذا ال
+
+394
+00:31:00,450 --> 00:31:04,540
+dilution في تخفيف أول tubeهذه كل الأفكار اللي ممكن
+
+395
+00:31:04,540 --> 00:31:10,100
+تيجي بالنسبة لمسائل الـ Serial Delusion نرجع
+
+396
+00:31:10,100 --> 00:31:16,200
+للمثال اللي في الملزمة أو في الـ Slides فهذا
+
+397
+00:31:16,200 --> 00:31:20,300
+المثال ذاكر أنه أخد 1ML من العينة يبقى هذه العينة
+
+398
+00:31:20,300 --> 00:31:26,600
+مش مخففة تمام في كل tube حط 9MLNormal saline اللي
+
+399
+00:31:26,600 --> 00:31:30,020
+هو الـ Diluent أخد واحد مئل من هايلت يوب يبقى
+
+400
+00:31:30,020 --> 00:31:33,680
+تخفيفها واحد على واحد زائد تسعة واحد اللي هي ال
+
+401
+00:31:33,680 --> 00:31:36,820
+sample على واحد ال sample زائد ال diluent تسعة
+
+402
+00:31:36,820 --> 00:31:41,540
+يبقى واحد على عشرة عشرة أسالب واحد وقلنا هاي مش
+
+403
+00:31:41,540 --> 00:31:45,790
+مخففة فبالتالي مش هضرفيه أي تخفيفتخفيف التيوب
+
+404
+00:31:45,790 --> 00:31:49,430
+التاني أخدت 1 ML من التيوب الأول على التيوب التاني
+
+405
+00:31:49,430 --> 00:31:54,290
+يبقى التخفيف 1 على 1 زائد 9 يبقى 1 على 10 في تخفيف
+
+406
+00:31:54,290 --> 00:31:57,890
+التيوب القابل 10 أقل سالب 1 يبقى 10 أقل سالب 2
+
+407
+00:31:57,890 --> 00:32:05,650
+وهكذا هكمل في كل تيوب ماخد من كل تيوب 1 ML تمام
+
+408
+00:32:05,650 --> 00:32:09,470
+أخد 1 ML من كل تيوب وزرعوا على إبلات قلنا هنلاحظ
+
+409
+00:32:09,470 --> 00:32:13,310
+أن أحنا في أول تيوبات هتكون two numerous to count
+
+410
+00:32:13,310 --> 00:32:18,500
+وآخر تيوب twoif you to count يبقى هدولة التانية
+
+411
+00:32:18,500 --> 00:32:21,800
+كانوا two many colonies to count مش هعود عنهم في
+
+412
+00:32:21,800 --> 00:32:28,180
+القانون هدول أقل من تلاتين تلت كولوني أخر تلت
+
+413
+00:32:28,180 --> 00:32:32,780
+تخفيفات أقل من تلتين فبالتالي هقول two if you to
+
+414
+00:32:32,780 --> 00:32:36,600
+count هذا ما بين التلتين والتلتمية فهو اللي هعوده
+
+415
+00:32:36,600 --> 00:32:40,040
+في القانون اللي هو مية و تسعة و خمسين كولوني كان
+
+416
+00:32:40,040 --> 00:32:43,700
+تخفيفه عشرة والسالب تلاتة هنعوده في القانون اللي
+
+417
+00:32:43,700 --> 00:32:50,160
+هومية مية و تسعة و خمسين على أس ال volume اللي أنا
+
+418
+00:32:50,160 --> 00:32:56,880
+أخدته اللي هو واحد على عشرة أسالب تلاتة طبعا عشرة
+
+419
+00:32:56,880 --> 00:33:00,520
+أسالب تلاتة بتطلع على فوق فبالتالي بتصير عشرة اف
+
+420
+00:33:00,520 --> 00:33:04,100
+تلاتة بتطلع بالنهاية القانون كله farming units per
+
+421
+00:33:04,100 --> 00:33:08,520
+milliliter لأنه كانت سائلة فبالتالي per milliliter
+
+422
+00:33:08,520 --> 00:33:12,960
+هاي هذا مثالبقولي هان روحتي إنتي عدتي لقولي في
+
+423
+00:33:12,960 --> 00:33:18,880
+الطبخ كان 46 بما أنه كان 46 يبقى بحطه في القانون
+
+424
+00:33:18,880 --> 00:33:22,740
+تمام ما بين ال 30 وال 300 يبقى أنا وقتها هان هعود
+
+425
+00:33:22,740 --> 00:33:25,940
+في القانون قال لي حطيتي 1 ml من ال bacterial
+
+426
+00:33:25,940 --> 00:33:31,170
+culture ل 99 ml من ال salineوأخدت من هذا الـ tube
+
+427
+00:33:31,170 --> 00:33:34,590
+واحد من عشرة ML وروحتي زرعتي يبقى الـ dilution
+
+428
+00:33:34,590 --> 00:33:38,530
+هيكون واحد على واحد زائد تسعة وتسعين اللي هي واحد
+
+429
+00:33:38,530 --> 00:33:42,430
+على مية يبقى هاي التخفيف واحد على مية الكل هو ستة
+
+430
+00:33:42,430 --> 00:33:46,470
+و أربعين القس اللي هي واحد من عشرة ML يبقى بعود في
+
+431
+00:33:46,470 --> 00:33:50,950
+القانون ستة و أربعين ضرب مية ليش لأنه ال dilution
+
+432
+00:33:50,950 --> 00:33:55,090
+أنا طلعته لفوق كان عشرة أس سالب اتنين طلعته لفوق
+
+433
+00:33:55,090 --> 00:33:59,860
+صار عشرة أس اتنين اللي هي في المية ضربالواحد على
+
+434
+00:33:59,860 --> 00:34:06,000
+واحد من عشرة الواحد من عشرة طلعت فا يبقى سارت عشرة
+
+435
+00:34:06,000 --> 00:34:11,340
+فهيك القانون بالنهاية ستة واربعين الف colony
+
+436
+00:34:11,340 --> 00:34:17,960
+farming unit per ml هذا بالنسبة لهذا
+
+437
+00:34:17,960 --> 00:34:22,360
+المثال هيك ما نكون خلصنا معمل ال cereal dilution
+
+438
+00:34:22,360 --> 00:34:27,810
+methodبيجي طبعا أسئلة مثال حسابات أحسبي اللي هو ال
+
+439
+00:34:27,810 --> 00:34:32,730
+colony farming unit في بير ميلي later في هذا ال في
+
+440
+00:34:32,730 --> 00:34:36,150
+هاي العينة وقولنا الأسئلة ممكن يكون ال dilution مش
+
+441
+00:34:36,150 --> 00:34:42,010
+موجود ممكن ال volume of blood مش موجود ممكنممكن ال
+
+442
+00:34:42,010 --> 00:34:46,550
+volume of sample أنت تحسبيه ممكن ال daily weight
+
+443
+00:34:46,550 --> 00:34:49,870
+أنت تحسبيه ممكن العينة تكون مش مخففة ممكن تكون
+
+444
+00:34:49,870 --> 00:34:53,670
+مخففة فهي كل الأمور بدنا ننتبه عليها برضه ممكن
+
+445
+00:34:53,670 --> 00:34:58,170
+يجيبلك عدد الكولوني أقل من تلاتين أو أكتر من
+
+446
+00:34:58,170 --> 00:35:02,170
+تلاتمية فبالتالي هانش هلجأ للتعويض في القانون هكتب
+
+447
+00:35:02,170 --> 00:35:07,410
+two few to count or two numerous to countهك مينكون
+
+448
+00:35:07,410 --> 00:35:11,510
+خلصنا الشعب تر يعطيكم العافية والسلام عليكم ورحمة
+
+449
+00:35:11,510 --> 00:35:12,570
+الله وبركاته
+
diff --git a/PL9fwy3NUQKwZWgAb_V1ATlRRsH-6i3cPC/lhmDRi5HdZw.srt b/PL9fwy3NUQKwZWgAb_V1ATlRRsH-6i3cPC/lhmDRi5HdZw.srt
new file mode 100644
index 0000000000000000000000000000000000000000..b92be2adca7d26119d030f8b7b222cc2d69600b7
--- /dev/null
+++ b/PL9fwy3NUQKwZWgAb_V1ATlRRsH-6i3cPC/lhmDRi5HdZw.srt
@@ -0,0 +1,702 @@
+1
+00:00:00,500 --> 00:00:03,740
+أتكلمنا سابقًا عن الـ spells والانسكابات وقلنا لو
+
+2
+00:00:03,740 --> 00:00:08,140
+صار عندي أي انسكاب أو أي انكسار بخبر الinstructor
+
+3
+00:00:08,140 --> 00:00:12,480
+عشان يعطيني التعليمات في التعامل معها سنتكلم في
+
+4
+00:00:12,480 --> 00:00:15,920
+هاي الـ slide كيف أنا بدي أتعامل مع الـ spells دي
+
+5
+00:00:15,920 --> 00:00:20,080
+contamination of small or moderate spells
+
+6
+00:00:20,080 --> 00:00:25,960
+مع الانسكاب اللي بكون صغير ومتوسط لأنه أنا
+
+7
+00:00:25,960 --> 00:00:29,990
+فيه عندي ممكن يكون large spills انسكاب كبير سيكون
+
+8
+00:00:29,990 --> 00:00:33,330
+عندي التعامل مختلف أول حاجة notify other
+
+9
+00:00:33,330 --> 00:00:37,530
+workers in the area of the spills بشعر وبخبر
+
+10
+00:00:37,530 --> 00:00:42,210
+الموجودين في المكان أن والله في شيء انكب في المكان
+
+11
+00:00:42,210 --> 00:00:45,290
+and control the traffic through the area وبدي أتحكم
+
+12
+00:00:45,290 --> 00:00:50,290
+في حركة المرور يعني بحكي لأي حد ممنوع أنه يقرب على
+
+13
+00:00:50,290 --> 00:00:55,770
+هذه المنطقة لأنه والله صار في عندي انسكاب shoe
+
+14
+00:00:55,770 --> 00:00:59,130
+covers and safety goggles بقول لك بتلبسي الـ shoe
+
+15
+00:00:59,130 --> 00:01:03,030
+covers نلبس غطاء الأحذية ليش؟ لأنه أنا خلال الـ
+
+16
+00:01:03,030 --> 00:01:06,850
+handling ممكن يكون والله صار في عندي touch بين
+
+17
+00:01:06,850 --> 00:01:11,730
+البكتيريا وهي البكتيريا تكون معدية تمام، فبالتالي حتى
+
+18
+00:01:11,730 --> 00:01:17,470
+أمنع نقل العدوى لكل مكان بروحه وكمان لحذائي الـ
+
+19
+00:01:17,470 --> 00:01:21,750
+shoes تبعي بستخدم اللي هو الـ shoe covers and safety
+
+20
+00:01:21,750 --> 00:01:25,850
+goggles اللي هي النظارات لأنه ممكن يكون نتيجة
+
+21
+00:01:25,850 --> 00:01:29,190
+الانسكاب صار في عندي رذاذ في الجو وبالتالي يدخل في
+
+22
+00:01:29,190 --> 00:01:33,210
+عيني ويسبب لي infection if a spill is on a floor
+
+23
+00:01:33,210 --> 00:01:36,350
+may be splashed beyond the immediate area of the spill
+
+24
+00:01:36,350 --> 00:01:40,450
+بقول لك ممكن الـ spill ينتشر كمان للمنطقة المجاورة
+
+25
+00:01:40,450 --> 00:01:44,330
+put on gloves بقول لك بعدها تلبسي الـ gloves and cover
+
+26
+00:01:44,330 --> 00:01:49,030
+the spill area with paper towels بغطي منطقة الانسكاب
+
+27
+00:01:49,030 --> 00:01:54,880
+بـ paper towels بعدها بـ disinfectant over the towel from
+
+28
+00:01:54,880 --> 00:02:00,220
+the edge of the spill to the center بغطي أو بحاول أـ skip
+
+29
+00:02:00,220 --> 00:02:05,100
+disinfectant اللي هو الـ detail  المرطب على اللي هو الـ
+
+30
+00:02:05,100 --> 00:02:09,880
+tissue paper اللي أنا حطيتهم وبحاول آخذ من الـ each
+
+31
+00:02:10,270 --> 00:02:15,370
+من الحواف حتى المركز ليش؟ حتى أني أمنع انتشار اللي
+
+32
+00:02:15,370 --> 00:02:20,850
+هو الانتشار الانسكاب لأنه لو جبت من المركز لبرا
+
+33
+00:02:20,850 --> 00:02:24,050
+معناه الكلام أني أنا جبت بكتيريا ووزعتها وصارت
+
+34
+00:02:24,050 --> 00:02:28,410
+الدائرة أكبر بقول لك كل الأدوات دي contaminants all
+
+35
+00:02:28,410 --> 00:02:32,830
+objects in spill area كل الأدوات اللي موجودة في
+
+36
+00:02:32,830 --> 00:02:37,140
+منطقة الانسكاب بحاول أني أنا أعقمها وأننظفها لأنه
+
+37
+00:02:37,140 --> 00:02:41,940
+ممكن يكون الرذاذ وقع عليها allow  بس تنتظر لمدة 30
+
+38
+00:02:41,940 --> 00:02:46,380
+دقيقة of contact time حتى هذه 30 دقيقة تكون كافية
+
+39
+00:02:46,380 --> 00:02:54,160
+حتى إنه يتم قتل كل شيء infectious، تمام؟ Pick
+
+40
+00:02:54,160 --> 00:02:58,620
+up any sharps including broken glass with forceps
+
+41
+00:02:58,620 --> 00:03:02,220
+and place in a sharps container بقول لك لو في أي مواد
+
+42
+00:03:02,220 --> 00:03:05,960
+حادة أو مواد زجاجية نتيجة الانكسار أو الانسكاب
+
+43
+00:03:06,300 --> 00:03:09,800
+بحاول أني ألتقطه بالـ forceps الـ forceps اللي هو
+
+44
+00:03:09,800 --> 00:03:13,620
+الملقط ممنوع أنا أستخدم إيدي لأنه ممكن يصير في
+
+45
+00:03:13,620 --> 00:03:18,560
+عندي infection ويصير systemic infection وتدخل
+
+46
+00:03:18,560 --> 00:03:22,320
+الدم ويسبب لي طبعًا أمراض عندي place in a sharps
+
+47
+00:03:22,320 --> 00:03:26,200
+container بعد ما أخلص أنا بحط هذه المواد الحادة و
+
+48
+00:03:26,200 --> 00:03:29,120
+الـ broken glass في الـ sharps container وشو هو الـ
+
+49
+00:03:29,120 --> 00:03:32,660
+sharps container هو نفسه الـ puncture-resistant
+
+50
+00:03:32,660 --> 00:03:35,890
+container اللي اتكلمنا عنه اللي قلنا أي مواد حادة أنا
+
+51
+00:03:35,890 --> 00:03:40,930
+بستخدمها اللي هي الملقط  المشروط كلها هاي بحطها فيه
+
+52
+00:03:40,930 --> 00:03:45,130
+puncture-resistant container container مقاوم للثقب
+
+53
+00:03:45,130 --> 00:03:48,310
+لأنه أنا لو حطيتها في autoclave bag هذا الـ case
+
+54
+00:03:48,310 --> 00:03:52,590
+ممكن يخترق الـ case الـ needle مثلًا المواد الحادة دي
+
+55
+00:03:52,590 --> 00:03:56,350
+ممكن تخترق الـ case وبالتالي تسبب لي  إصابة وتعَملي
+
+56
+00:03:56,350 --> 00:04:00,960
+infection Use a dustpan and brush بقول لك هان
+
+57
+00:04:00,960 --> 00:04:05,120
+استخدمي اللي هي معناه هاي الـ dustpan and brush
+
+58
+00:04:05,120 --> 00:04:10,340
+هي الممسحة to recover any shards of broken glass
+
+59
+00:04:10,340 --> 00:04:15,100
+in contaminated liquid نستخدم الممسحة حتى نتخلص من
+
+60
+00:04:15,100 --> 00:04:19,960
+أي شظايا من الزجاج بعد ما أخلص من الممسحة طبعًا ما
+
+61
+00:04:19,960 --> 00:04:23,600
+بمسك الممسحة وبحطها وبـ أرميها في أي مكان لأ بقول لك
+
+62
+00:04:23,600 --> 00:04:29,640
+ضروري دي و contaminated بنحطها في مطهر عشان ما يكون
+
+63
+00:04:29,640 --> 00:04:33,900
+فيها بكتيريا wipe area with disinfectant and clean
+
+64
+00:04:33,900 --> 00:04:37,320
+paper towels and put in biohazard bag بعد ما أخلص
+
+65
+00:04:37,320 --> 00:04:43,200
+بغطي المنطقة بـ tissue paper بمطهر ومعقم وبعد ما أخلص
+
+66
+00:04:43,200 --> 00:04:46,620
+بحط هذه الأدوات اللي استخدمتها في الـ biohazard bag
+
+67
+00:04:46,620 --> 00:04:51,200
+الـ biohazard bag هي نفسها الـ autoclave bag لأن
+
+68
+00:04:51,200 --> 00:04:54,460
+tissue paper مش شيء  فبحطه في الـ autoclave bag
+
+69
+00:04:55,090 --> 00:04:59,750
+move if spill is on the floor طبعًا بمسح المنطقة أو
+
+70
+00:04:59,750 --> 00:05:03,370
+بمسح الأرضية لو كان الـ spill على الأرضية remove
+
+71
+00:05:03,370 --> 00:05:07,010
+gloves and shoe covers before leaving the area of the
+
+72
+00:05:07,010 --> 00:05:11,350
+spill بقول لك قبل ما تطلعي من منطقة الانسكاب بتتخلصي
+
+73
+00:05:11,350 --> 00:05:16,550
+من الـ gloves ومن غطاء الحذاء وبتحطي في الـ bio
+
+74
+00:05:16,550 --> 00:05:20,830
+hazard اللي هو الـ autoclave bag حتى إني أنا أمنع
+
+75
+00:05:20,830 --> 00:05:25,810
+انتشار طبعًا اللي هو العدوى بعد ما أخلص بغسل إيدي
+
+76
+00:05:25,810 --> 00:05:30,250
+بالمياه والصابون هذا بالنسبة لـ small and moderate
+
+77
+00:05:30,250 --> 00:05:34,510
+spills أما بالنسبة لـ large spills الـ large spills
+
+78
+00:05:34,510 --> 00:05:39,670
+بقول لك evacuate the room بدك تخلي الغرفة close the door بدك
+
+79
+00:05:39,670 --> 00:05:43,560
+تسكري الباب and prevent others from entering و
+
+80
+00:05:43,560 --> 00:05:47,700
+بدك تمنعي أي حد أنه يدخل هذه الغرفة and wait
+
+81
+00:05:47,700 --> 00:05:52,840
+بدك تنتظري 30 دقيقة حتى أن الرذاذ يستقر على الأرض
+
+82
+00:05:52,840 --> 00:05:57,460
+والأجهزة عشان أنا لما أدخل ما يكون في شيء موجود فيه
+
+83
+00:05:57,460 --> 00:06:00,660
+الهواء وأنا أستنشقه ويقلقش المخاطرية ويسبب لي
+
+84
+00:06:00,660 --> 00:06:05,230
+infection مش أستنى تلاتين في الـ اتكلمنا إحنا في
+
+85
+00:06:05,230 --> 00:06:08,530
+الـ small وفي الـ moderate spills تلاتين دقيقة حتى
+
+86
+00:06:08,530 --> 00:06:13,150
+تكون كافية لقتل الـ اللي هو البكتيريا لكن هان
+
+87
+00:06:13,150 --> 00:06:16,950
+بدي تلاتين دقيقة حتى إني أنا أضمن أن الرذاذ
+
+88
+00:06:16,950 --> 00:06:21,610
+استقر على الأرض وعلى الأجهزة بعدها بأتبع نفس
+
+89
+00:06:21,610 --> 00:06:25,550
+الخطوات اللي أنا استخدمتها في الـ small والـ
+
+90
+00:06:25,550 --> 00:06:29,030
+moderate spills بما فيهم آخر شيء إني أنا أنتظر كمان
+
+91
+00:06:29,030 --> 00:06:33,990
+تلاتين دقيقة حتى إنها تكون كافية إنها تقتل كل
+
+92
+00:06:33,990 --> 00:06:38,030
+البكتيريا هذه بعض الملاحظات بالنسبة لـ بالمعمل
+
+93
+00:06:38,030 --> 00:06:42,230
+إن ممنوع حد يدخل المعمل بدون أي أحد من الأساتذة
+
+94
+00:06:42,650 --> 00:06:46,770
+ممنوع أني أنا الأدوات الشخصية أني أحطها في منطقة
+
+95
+00:06:46,770 --> 00:06:49,730
+الشغل جوالات ممنوع الجوالات في منطقة الشغل ممنوع
+
+96
+00:06:49,730 --> 00:06:53,230
+الأدوات الشخصية من لو أنا اضطرت أني أنا أستخدم أي
+
+97
+00:06:53,230 --> 00:06:58,800
+قلم ودفتر بعد ما أخلص أحاول أني أنا أعقمهم كل شعب
+
+98
+00:06:58,800 --> 00:07:02,920
+هتتقسم لجروبات كل جروب هيكون طبعًا متكون من أربع
+
+99
+00:07:02,920 --> 00:07:08,060
+بنات كل هدول الأربع بنات أو هدول الجروب هيدولوا طول
+
+100
+00:07:08,060 --> 00:07:12,040
+الفصل يشتغل نفس الشغل ويكتب حتى أنه يكون الناتج
+
+101
+00:07:12,040 --> 00:07:16,060
+التقارير تكون نفسها بالنسبة للأشياء المحتاجة في
+
+102
+00:07:16,060 --> 00:07:19,820
+المعمل الـ lab coat ضروري جدًا جدًا الـ Match أو الـ
+
+103
+00:07:19,820 --> 00:07:22,520
+lighter  الولاعة أنا أكيد معمل الـ Microbiology
+
+104
+00:07:22,520 --> 00:07:25,700
+هنستخدم  الولاعة فبالتالي ضروري كل group يكون معه
+
+105
+00:07:25,700 --> 00:07:29,520
+أداة الصابون بدنا قولنا عن الـ safety rules بدنا
+
+106
+00:07:29,520 --> 00:07:33,340
+نغسل أيدينا قبل وبعد الشغل بمياه والصابون الماركر
+
+107
+00:07:33,340 --> 00:07:37,020
+ضروري برضه كل group يكون معه ماركر small أو
+
+108
+00:07:37,020 --> 00:07:40,780
+medium size مناشف صغيرة حتى إني أنا بعد
+
+109
+00:07:40,780 --> 00:07:46,060
+ما أغسل إيدي أنشفهم  saline plastic bag هي إنها
+
+110
+00:07:46,060 --> 00:07:49,100
+هتكون حقيبة حتى إني أنا أحط فيها الـ lab coat ممكن
+
+111
+00:07:49,100 --> 00:07:51,980
+كيس أنك تحط فيه الـ lab coat عشان تفصليه عن أدواتك
+
+112
+00:07:51,980 --> 00:07:55,360
+التانية وما يصيبك فيه أي infection rubber band
+
+113
+00:07:55,360 --> 00:07:59,040
+اللي هي مطاط  المطاطة هي ممكن أنا أستخدمها في حال
+
+114
+00:07:59,040 --> 00:08:03,560
+كان الـ lab coat اللي يكون الأكمام واسعة فهيك ممكن
+
+115
+00:08:03,560 --> 00:08:07,740
+يعيق الشغل فأنا ممكن أحط الـ rubber band حتى تقدر
+
+116
+00:08:07,740 --> 00:08:12,250
+تشتغلي بكل سهولة General Microbiology Manual هذا
+
+117
+00:08:12,250 --> 00:08:16,550
+الكتاب الملزمة اللي حكينا عنها هي الملزمة نزلناها
+
+118
+00:08:16,550 --> 00:08:22,830
+على الـ moodle موجودة إن شاء الله لما نشتغل الشغل
+
+119
+00:08:22,830 --> 00:08:26,150
+لما نبدأ بالشغل العملي هنتكلم عنها لأنه فيها هيكون
+
+120
+00:08:26,150 --> 00:08:30,770
+كتابة تقارير بالنسبة لـ Instruments in Microbiology
+
+121
+00:08:30,770 --> 00:08:33,650
+Lab الأدوات اللي أنا بستخدمها في معمل الـ
+
+122
+00:08:33,650 --> 00:08:37,420
+Microbiology أول أداة اللي هي الـ incubator الـ
+
+123
+00:08:37,420 --> 00:08:42,600
+incubator هو عبارة عن حاضنة معناها الحاضنة الـ
+
+124
+00:08:42,600 --> 00:08:46,480
+incubator هي عبارة عن جهاز بيوفر لي ظروف مناسبة
+
+125
+00:08:46,480 --> 00:08:49,140
+لنمو البكتيريا اللي بقى لو بدي أعرف الـ incubator
+
+126
+00:08:49,140 --> 00:08:53,540
+بحكي device which provides appropriate conditions
+
+127
+00:08:53,540 --> 00:08:57,180
+for bacterial growth بيوفر ظروف مناسبة لنمو
+
+128
+00:08:57,180 --> 00:08:59,760
+البكتيريا غالبًا درجة الحرارة اللي بتنمو عليها
+
+129
+00:08:59,760 --> 00:09:02,520
+البكتيريا سبعة وثلاثين  هيكون مكتوب درجة
+
+130
+00:09:02,520 --> 00:09:06,640
+الحرارة سبعة وثلاثين لما أشوف 37 هيك أنا مضمن أن
+
+131
+00:09:06,640 --> 00:09:10,280
+والله في عندي 37 مناسبة لنمو البكتيريا بنحط
+
+132
+00:09:10,280 --> 00:09:14,160
+الأطباق اللي بتزرع هنا وبعدين بنسكر بعد 24 ساعة
+
+133
+00:09:14,160 --> 00:09:17,300
+بنلاحظ نمو البكتيريا طبعًا هذا الـ incubator يكون
+
+134
+00:09:17,300 --> 00:09:22,460
+يوفر ظروف هوائية الـ autoclave هو عبارة عن جهاز
+
+135
+00:09:22,460 --> 00:09:27,320
+بنستخدمه used for sterilization لتعقيم  باستخدام
+
+136
+00:09:27,320 --> 00:09:30,980
+الضغط  إذًا ملاحظين بيوفر لي درجة حرارة عالية وضغط
+
+137
+00:09:30,980 --> 00:09:35,340
+عالي كافي إنه يقتل لي all microorganisms or all
+
+138
+00:09:35,340 --> 00:09:39,600
+البكتيريا يعني يقتل لي كل البكتيريا بالنسبة لي الـ
+
+139
+00:09:39,600 --> 00:09:42,220
+anaerobic jar وفيه عندي الـ candle jar إحنا قلنا
+
+140
+00:09:42,220 --> 00:09:45,560
+فيه بكتيريا طبعًا بتختلف في خصائصها فيه بكتيريا
+
+141
+00:09:45,560 --> 00:09:48,420
+بتحتاج ظروف لاهوائية في النمو فيه بتحتاج ظروف
+
+142
+00:09:48,420 --> 00:09:51,580
+هوائية لو أنا البكتيريا بتحتاج ظروف هوائية في الـ
+
+143
+00:09:51,580 --> 00:09:54,980
+incubator العادي هيك بيوفر لي ظروف هوائية وظروف 37
+
+144
+00:09:54,980 --> 00:09:59,570
+مناسبة للنمو لكن لو البكتيريا لاهوائية الـ autoclave
+
+145
+00:09:59,570 --> 00:10:02,550
+ما بنمي  البكتيريا فبالتالي أنا
+
+146
+00:10:02,550 --> 00:10:07,060
+بحتاج إلى الـ anaerobic jar هذا بيوفر لي الظروف
+
+147
+00:10:07,060 --> 00:10:11,020
+اللاهوائية هو عبارة عن مرتبان زجاجي بيكون محكم
+
+148
+00:10:11,020 --> 00:10:14,260
+الإغلاق موجود فيه case هذا الـ case فيه مواد
+
+149
+00:10:14,260 --> 00:10:19,800
+كيميائية من جوا بالنهاية بتكون نواتج هذه النواتج
+
+150
+00:10:19,800 --> 00:10:23,800
+عبارة عن بتوفر ظروف لاهوائية نتعرف عليها بالتفصيل
+
+151
+00:10:23,800 --> 00:10:28,940
+في معمل الـ Bacterial Oxygen Requirements بالتالي
+
+152
+00:10:28,940 --> 00:10:32,340
+آخر شيء بتوفر ظروف لاهوائية مناسبة لنمو البكتيريا
+
+153
+00:10:32,680 --> 00:10:38,100
+بعد ما أنا طبعًا أسكر هذا أحط الأطباق البكتيرية
+
+154
+00:10:38,100 --> 00:10:44,040
+اللي أنا بحتاجها حتى تنمو وأسكر المرتبان بحطها في
+
+155
+00:10:44,040 --> 00:10:48,380
+الـ incubator العادي هيك أنا بتكون موفرة ظروف 37 وظروف
+
+156
+00:10:48,380 --> 00:10:52,360
+ظروف لاهوائية الـ candle jar من اسمها candle شمعة
+
+157
+00:10:52,360 --> 00:10:57,020
+لأني بستخدم شمعة في هذا الـ candle jar فيه بكتيريا
+
+158
+00:10:57,020 --> 00:11:01,790
+حتى تنمو تحت ظروف الـ CO2 حتى أنا أوفر ظروف الـ
+
+159
+00:11:01,790 --> 00:11:07,470
+CO2 بجيب جار محكم الإغلاق هذا الـ jar بتكون من
+
+160
+00:11:07,470 --> 00:11:11,430
+أطباق بكتيرية بحط الأطباق اللي أنا بدي أعزل
+
+161
+00:11:11,430 --> 00:11:16,950
+البكتيريا اللي بتحتاج CO2 وأثبت آخر شيء slide حتى
+
+162
+00:11:16,950 --> 00:11:20,750
+أحط slide حتى أثبت عليها الشمعة وأضوي الشمعة و
+
+163
+00:11:20,750 --> 00:11:26,090
+بسكر هذا المرتبان لما تطفأ الشمعة هيك أنا بتأكد أنه
+
+164
+00:11:26,090 --> 00:11:29,770
+والله اتوفرت ظروف CO2 بحمل هذا الـ jar وبحطه في
+
+165
+00:11:29,770 --> 00:11:34,270
+الـ incubator العادي هيك أنا توفرت عندي ظروف 37 الـ
+
+166
+00:11:34,270 --> 00:11:38,730
+CO2 اللازم لنمو البكتيريا بنسن برنر اللي هو اللهب
+
+167
+00:11:38,730 --> 00:11:43,410
+بنسن اللهب بنسن ضروري جدًا لحتى إنه خلال شغلي أنا
+
+168
+00:11:43,410 --> 00:11:47,330
+في الـ micro لازم يكون اللهب مشغل حتى إني أنا أوفر
+
+169
+00:11:47,330 --> 00:11:50,770
+ظروف معقمة أمنع الـ contamination باستخدم برضه
+
+170
+00:11:50,770 --> 00:11:54,070
+اللهب في تعقيم الـ inoculating loop الـ loop هاي هي
+
+171
+00:11:54,070 --> 00:11:57,550
+الأداة اللي أنا بستخدمها في زراعة البكتيريا في
+
+172
+00:11:57,550 --> 00:12:01,210
+عندنا inoculating loop هذا مش disposable ممكن أنا أستخدمه
+
+173
+00:12:01,210 --> 00:12:05,270
+أكثر من مرة ممكن أعقمه، بعقمه أو لازم إني أنا
+
+174
+00:12:05,270 --> 00:12:09,730
+أعقمه باللهب بنسن بحطه على اللهب لما يتوهج ويصير
+
+175
+00:12:09,730 --> 00:12:14,290
+لونه أحمر هيك بعرف إنه صار sterile، صار معقم ممكن
+
+176
+00:12:14,290 --> 
\ No newline at end of file
diff --git a/PL9fwy3NUQKwZWgAb_V1ATlRRsH-6i3cPC/lhmDRi5HdZw_postprocess.srt b/PL9fwy3NUQKwZWgAb_V1ATlRRsH-6i3cPC/lhmDRi5HdZw_postprocess.srt
new file mode 100644
index 0000000000000000000000000000000000000000..cab0a0fb13a44715ce6d301baa6c8d1bbbee3474
--- /dev/null
+++ b/PL9fwy3NUQKwZWgAb_V1ATlRRsH-6i3cPC/lhmDRi5HdZw_postprocess.srt
@@ -0,0 +1,776 @@
+1
+00:00:00,500 --> 00:00:03,740
+أتكلمنا سابقًا عن الـ Spells والانثكابات وقلنا لو
+
+2
+00:00:03,740 --> 00:00:08,140
+صار عندي أي انثكاب أو أي انكسار بخبر ال instructor
+
+3
+00:00:08,140 --> 00:00:12,480
+عشان يعطيني التعليمات في التعامل معها هنتكلم في
+
+4
+00:00:12,480 --> 00:00:15,920
+هاي ال slide كيف أنا بدي أتعامل مع ال Spells دي
+
+5
+00:00:15,920 --> 00:00:20,080
+contamination of spells of small or moderate
+
+6
+00:00:20,080 --> 00:00:25,960
+spells مع الانثكاب اللي بكون صغير ومتوسط لأنه أنا
+
+7
+00:00:25,960 --> 00:00:29,990
+فيه عنديممكن يكون large spells انسكاب كبير هيكون
+
+8
+00:00:29,990 --> 00:00:33,330
+في ياندي التعامل مختلف اول حاجة notify other
+
+9
+00:00:33,330 --> 00:00:37,530
+workers in the area of the spells بشعر و بخبر
+
+10
+00:00:37,530 --> 00:00:42,210
+الموجودين في المكان ان والله في إشي انكب في المكان
+
+11
+00:00:42,210 --> 00:00:45,290
+and control the traffic through area و بدي أتحكم
+
+12
+00:00:45,290 --> 00:00:50,290
+في حركة المرور يعني بحكي لأي حد ممنوع انه يقرب على
+
+13
+00:00:50,290 --> 00:00:55,770
+هذه المنطقة لإنه والله صار في ياندي انسكابshoe
+
+14
+00:00:55,770 --> 00:00:59,130
+cover and safety goggles بقولك بتلبسي ال shoe
+
+15
+00:00:59,130 --> 00:01:03,030
+covers نلبس الغطاء الأحذية ليش لأنه أنا خلال ال
+
+16
+00:01:03,030 --> 00:01:06,850
+handicap ممكن يكون والله صار في عندي touch بين
+
+17
+00:01:06,850 --> 00:01:11,730
+بكتيريا وهي البكتيريا تكون معدية تمام فبالتالي حتى
+
+18
+00:01:11,730 --> 00:01:17,470
+أمنع نقل العدو لكل مكان بروحه وكمان لحذائي ل ال
+
+19
+00:01:17,470 --> 00:01:21,750
+shoes تبعي بستخدم اللي هو ال shoe cover and safety
+
+20
+00:01:21,750 --> 00:01:25,850
+goggles اللي هي النضارات لأنه ممكن يكوننتيجة
+
+21
+00:01:25,850 --> 00:01:29,190
+الانسكاب صار في عندي رداد في الجو وبالتالي يدخل في
+
+22
+00:01:29,190 --> 00:01:33,210
+عيني ويسببلي infection if a spill is on a floor
+
+23
+00:01:33,210 --> 00:01:36,350
+may be splashed beyond immediate area of the spill
+
+24
+00:01:36,350 --> 00:01:40,450
+بقولك ممكن ال spill ينتشر كمان للمنطقة المجاورة
+
+25
+00:01:40,450 --> 00:01:44,330
+put on gloves بقولك بعدها تلبسي ل gloves and cover
+
+26
+00:01:44,330 --> 00:01:49,030
+spill area with paper towel بغطي منطقة الانسكاب
+
+27
+00:01:49,030 --> 00:01:54,880
+بتشوبابربعدها بور disinfectant over towel from
+
+28
+00:01:54,880 --> 00:02:00,220
+each of the spill to center بغطي او بحاول ا skip
+
+29
+00:02:00,220 --> 00:02:05,100
+disinfectant اللي هو ال detail متهر على اللي هو ال
+
+30
+00:02:05,100 --> 00:02:09,880
+tissue paper اللي انا حطيتهم وبحاول اخد من ال each
+
+31
+00:02:10,270 --> 00:02:15,370
+من الحواف حتى المركز ليش حتى أني أمنع انتشار اللي
+
+32
+00:02:15,370 --> 00:02:20,850
+هو الانتشار الانسكاب لأني لو جبت من المركز لبرا
+
+33
+00:02:20,850 --> 00:02:24,050
+معناه الكلام أني أنا جبت بكتيريا ووزعتها وصارت
+
+34
+00:02:24,050 --> 00:02:28,410
+الدائرة أكبر بقولك كل الأدوات دي كونتمانات all
+
+35
+00:02:28,410 --> 00:02:32,830
+object in spell area كل الأدوات اللي موجودة في
+
+36
+00:02:32,830 --> 00:02:37,140
+منطقة الانسكاب بحاول أني أنا أعقمها و أنظفهالأنه
+
+37
+00:02:37,140 --> 00:02:41,940
+ممكن يكون الرذاذ إيجا عليها allow بس تنتظر لمدة 30
+
+38
+00:02:41,940 --> 00:02:46,380
+دقيقة of contact time حتى هذه 30 دقيقة تكون كافية
+
+39
+00:02:46,380 --> 00:02:54,160
+حتى إنه يتم قتل كل شي Infectious، تمام؟Pick
+
+40
+00:02:54,160 --> 00:02:58,620
+up any charp including broken glass with forceps
+
+41
+00:02:58,620 --> 00:03:02,220
+and place in char container بقولك لو في أي مواد
+
+42
+00:03:02,220 --> 00:03:05,960
+حادة أو مواد زجاجية نتيجة الانكسار أو الانسكاب
+
+43
+00:03:06,300 --> 00:03:09,800
+بحاول أني ألتقطه بالـ forceps الـ forceps اللي هو
+
+44
+00:03:09,800 --> 00:03:13,620
+الملقط ممنوع أنا أستخدم إيدي لأنه ممكن يصير في
+
+45
+00:03:13,620 --> 00:03:18,560
+عندي infection و يصير systemic infection و تدخل
+
+46
+00:03:18,560 --> 00:03:22,320
+الدم و يسبب لي طبعا أمراض عندي place in a charred
+
+47
+00:03:22,320 --> 00:03:26,200
+container بعد ما هذا أنا بحط هذه المواد الحادة و
+
+48
+00:03:26,200 --> 00:03:29,120
+ال broken glass في ال charred container وشو هو ال
+
+49
+00:03:29,120 --> 00:03:32,660
+charred container هو نفسه ال puncture resistant
+
+50
+00:03:32,660 --> 00:03:35,890
+container اللي اتكلمنا عنه اللي قلناأي مواد حد أنا
+
+51
+00:03:35,890 --> 00:03:40,930
+بستخدمها اللي هي الملقط المشروط كلها هاي بحطها بيه
+
+52
+00:03:40,930 --> 00:03:45,130
+puncture resistant container container مقاول للخرق
+
+53
+00:03:45,130 --> 00:03:48,310
+لأنه انا لو حطيتها في autoclave bag هذا ال case
+
+54
+00:03:48,310 --> 00:03:52,590
+ممكن يخترق ال case ال needle مثلا المواد الحد ده
+
+55
+00:03:52,590 --> 00:03:56,350
+ممكن تخترق ال case وبالتالي تسببلي الأداء و تعملي
+
+56
+00:03:56,350 --> 00:04:00,960
+infectionUse a sewage and dust pan بقولك هان
+
+57
+00:04:00,960 --> 00:04:05,120
+استخدمي اللي هي معناه هاي ال sewage and dust pan
+
+58
+00:04:05,120 --> 00:04:10,340
+هي الممسحة to recover any charred of broken glass
+
+59
+00:04:10,340 --> 00:04:15,100
+in contaminated liquid نستخدم الممسح حتى نتخلص من
+
+60
+00:04:15,100 --> 00:04:19,960
+أي شضايا من الزجاج بعد ما أخلص من الممسح طبعا ما
+
+61
+00:04:19,960 --> 00:04:23,600
+بمسك الممسحة و بحطها و برميها في أي مكان لأ بقولك
+
+62
+00:04:23,600 --> 00:04:29,640
+ضروري دي و contaminatedبنحطها في مطهر عشان ما يكون
+
+63
+00:04:29,640 --> 00:04:33,900
+فيها بكتيريا wipe area with disinfectant and clean
+
+64
+00:04:33,900 --> 00:04:37,320
+paper towel and pot in biohazard bag بعد ما أخلص
+
+65
+00:04:37,320 --> 00:04:43,200
+بغطي المنطقة بإيتيشو بابر بمطهر ومعقم وبعد ما أخلص
+
+66
+00:04:43,200 --> 00:04:46,620
+بحط هذه الأدوات اللي استخدمتها في ال biohazard bag
+
+67
+00:04:46,620 --> 00:04:51,200
+ال biohazard bag هي نفسها ال auto-clav bag لأن
+
+68
+00:04:51,200 --> 00:04:54,460
+إيتيشو بابر مش إشيحات فبحطه في ال auto-clav bag
+
+69
+00:04:55,090 --> 00:04:59,750
+move if spill is in floor طبعا بمسح المنطقة أو
+
+70
+00:04:59,750 --> 00:05:03,370
+بمسح الأرضية لو كان ال spill على الأرضية remove
+
+71
+00:05:03,370 --> 00:05:07,010
+gloves and shoes cover before removing area of the
+
+72
+00:05:07,010 --> 00:05:11,350
+spill بقولك قبل ما تطلع من منطقة الانسكاب بتتخلصي
+
+73
+00:05:11,350 --> 00:05:16,550
+من ال gloves و من الغطاء الحذاء و بتحطي في ال bio
+
+74
+00:05:16,550 --> 00:05:20,830
+hazardاللي هو الـ Autoclave bag حتى اني انا امنع
+
+75
+00:05:20,830 --> 00:05:25,810
+انتشار طبعا اللي هو العدوى بعد ما اخلص بغسل ايدي
+
+76
+00:05:25,810 --> 00:05:30,250
+بالميه والصابون هذا بالنسبة لـ Small and Moderate
+
+77
+00:05:30,250 --> 00:05:34,510
+Spills اما بالنسبة لـ Large Spill الـ Large Spill
+
+78
+00:05:34,510 --> 00:05:39,670
+بقولك evacuate room بدك تخلي الغرفة close door بدك
+
+79
+00:05:39,670 --> 00:05:43,560
+تسكري البوابand prevent others from entering و
+
+80
+00:05:43,560 --> 00:05:47,700
+بديك تمنعي أدح أي حد أنه يدخل هذه الغرفة and where
+
+81
+00:05:47,700 --> 00:05:52,840
+بديك تنتظر 30 دقيقة حتى أنه الرذاذ يستقر على الأرض
+
+82
+00:05:52,840 --> 00:05:57,460
+و الأجهزة عشان أنا لما أدخل مايكون إشي موجود فيه
+
+83
+00:05:57,460 --> 00:06:00,660
+الهواء و أنا أستنشقه و يقلقش المخاطرية و يسببلي
+
+84
+00:06:00,660 --> 00:06:05,230
+infectionمش أستنى تلاتين في ال .. اتكلمنا احنا في
+
+85
+00:06:05,230 --> 00:06:08,530
+الـ small و في ال moderate spells تلاتين دقيقة حتى
+
+86
+00:06:08,530 --> 00:06:13,150
+تكون كافية لقتل ال .. اللي هو البكتيريا لكن هان
+
+87
+00:06:13,150 --> 00:06:16,950
+بدي تلاتين دقيقة حتى اني انا اضمن انه ال radar
+
+88
+00:06:16,950 --> 00:06:21,610
+استقر على الأرض و على الأجهزة بعدها باتبع نفس
+
+89
+00:06:21,610 --> 00:06:25,550
+الخطوات اللي انااستخدمتها في الـ small و ال
+
+90
+00:06:25,550 --> 00:06:29,030
+moderate spells بما فيهم اخر شئ اني انا انتظر كمان
+
+91
+00:06:29,030 --> 00:06:33,990
+تلاتين دقيقة حتى انها تكون كافية انها تقتل كل
+
+92
+00:06:33,990 --> 00:06:38,030
+البكتيريا هذه بعض الملاحظات بالنسبة ل .. بالمعمل
+
+93
+00:06:38,030 --> 00:06:42,230
+ان ممنوح حد يدخل المعمل بدون اي حد من الاساتذة
+
+94
+00:06:42,650 --> 00:06:46,770
+الممنوع أنه أنا الأدوات الشخصية أني أحطها في منطقة
+
+95
+00:06:46,770 --> 00:06:49,730
+الشغل جوالات ممنوع الجوالات في منطقة الشغل ممنوع
+
+96
+00:06:49,730 --> 00:06:53,230
+الأدوات الشخصية من لو أنا اضطرت أني أنا أستخدم أي
+
+97
+00:06:53,230 --> 00:06:58,800
+قلمدفتر بعد ما أخلص أحاول أني أنا أعقمهم كل شعب
+
+98
+00:06:58,800 --> 00:07:02,920
+هتتقسم لجروبات كل جروب هيكون طبعا متكون من أربع
+
+99
+00:07:02,920 --> 00:07:08,060
+بنات كل هدول الأربع بنات أو هدول الجروب هيدلوا طول
+
+100
+00:07:08,060 --> 00:07:12,040
+الفصل يشتغل نفس الشغل و يكتب حتى أنه يكون النتاج
+
+101
+00:07:12,040 --> 00:07:16,060
+التقارير تكون نفسها بالنسبة للأشياء المحتاجة في
+
+102
+00:07:16,060 --> 00:07:19,820
+المعمل ال lab code ضروري جدا جداالـ Match أو الـ
+
+103
+00:07:19,820 --> 00:07:22,520
+Lighter الأداحة أنا أكيد معملة الـ Microbiology
+
+104
+00:07:22,520 --> 00:07:25,700
+هنستخدم اللها فبالتالي ضروري كل group يكون معاه
+
+105
+00:07:25,700 --> 00:07:29,520
+أداحة الصابونة بدنا قولنا عن ال safety rules بدنا
+
+106
+00:07:29,520 --> 00:07:33,340
+نغسل أديانا قبل و بعد الشغل بميه والصابون الماركر
+
+107
+00:07:33,340 --> 00:07:37,020
+ضروري برضه كل group يكون معاه ماركر Small أو
+
+108
+00:07:37,020 --> 00:07:40,780
+Medium Size دوال منشفة تكون صغيرة حتى أني أنا بعد
+
+109
+00:07:40,780 --> 00:07:46,060
+ما أغسل أديا أنشفهمساليابول بلاستيك باك هى انه
+
+110
+00:07:46,060 --> 00:07:49,100
+هتكون حقيبة حتى اني انا احط فيها ال lab coat ممكن
+
+111
+00:07:49,100 --> 00:07:51,980
+كيس انك تحط فيه ال lab coat عشان تفصليه عن أدواتك
+
+112
+00:07:51,980 --> 00:07:55,360
+التانية و ما يصف يد فيه اننا infection rubber band
+
+113
+00:07:55,360 --> 00:07:59,040
+اللى هى مطاط المطاطة هى ممكن انا استخدمها في حال
+
+114
+00:07:59,040 --> 00:08:03,560
+كان ال lab coat اللى يكون الاديان واسعين فهيك ممكن
+
+115
+00:08:03,560 --> 00:08:07,740
+يعيق الشغل فانا ممكن احط ال rubber band حتى تقدر
+
+116
+00:08:07,740 --> 00:08:12,250
+تشتغلي بكل سهولةGeneral Microbiology Manual هذا
+
+117
+00:08:12,250 --> 00:08:16,550
+الكتاب الملزمة اللي حكينا عنها هي الملزمة نزلناها
+
+118
+00:08:16,550 --> 00:08:22,830
+على الـ Model موجودة ان شاء الله لما نشتغل الشغل
+
+119
+00:08:22,830 --> 00:08:26,150
+لما نبدأ بالشغل العملي هنتكلم عنها لأنه فيها هيكون
+
+120
+00:08:26,150 --> 00:08:30,770
+كتابة تقارير بالنسبة لـ Instrument in Microbiology
+
+121
+00:08:30,770 --> 00:08:33,650
+Lab الأدوات اللي أنا بستخدمها في معمل الـ
+
+122
+00:08:33,650 --> 00:08:37,420
+Microbiologyأول أداة اللي هي ال incubator ال
+
+123
+00:08:37,420 --> 00:08:42,600
+incubator هو عبارة عن حاضنة معناها الحاضنة ال
+
+124
+00:08:42,600 --> 00:08:46,480
+incubator هي عبارة عن جهاز بوفر لي ظروف مناسبة
+
+125
+00:08:46,480 --> 00:08:49,140
+لنمو البكتيريا اللي بقى لو بدي أعرف ال incubator
+
+126
+00:08:49,140 --> 00:08:53,540
+بحكي device which confirm appropriate condition
+
+127
+00:08:53,540 --> 00:08:57,180
+for bacterial growth بيوفر ظروف مناسبة لنمو
+
+128
+00:08:57,180 --> 00:08:59,760
+البكتيريا غالبا درجة الحرارة اللي بتنمو عليها
+
+129
+00:08:59,760 --> 00:09:02,520
+البكتيريا سبعة و تلاتين هن بيكون مكتوب درجة
+
+130
+00:09:02,520 --> 00:09:06,640
+الحرارة سبعة و تلاتينلما اشوف 37 هك انا مضمن ان
+
+131
+00:09:06,640 --> 00:09:10,280
+والله في عندي 37 مناسبة لنمو البكتيريا بنحط
+
+132
+00:09:10,280 --> 00:09:14,160
+الأطباق اللي بتردش هنا وبعدين بنسكر بعد 24 ساعة
+
+133
+00:09:14,160 --> 00:09:17,300
+بنلاحظ نمو البكتيريا طبعا هذا ال incubator يكون
+
+134
+00:09:17,300 --> 00:09:22,460
+يوفر ظروف هوائية ال auto-clef هو عبارة عن جهاز
+
+135
+00:09:22,460 --> 00:09:27,320
+بنستخدمه used for sterilization لا تعقيمزي تنجرة
+
+136
+00:09:27,320 --> 00:09:30,980
+الضغط إذا ملاحظين بيوفرلي درجة حرارة عالية وضغط
+
+137
+00:09:30,980 --> 00:09:35,340
+عالي كافي إنه يقتللي all microorganism or all
+
+138
+00:09:35,340 --> 00:09:39,600
+بكتيريا يعني يقتللي كل البكتيريا بالنسبالي الـ
+
+139
+00:09:39,600 --> 00:09:42,220
+Anaerobic Jar و فيه اندي الـ Candle Jar احنا قولنا
+
+140
+00:09:42,220 --> 00:09:45,560
+فيه بكتيريا طبعا بتختلف في خصائصها فيه بكتيريا
+
+141
+00:09:45,560 --> 00:09:48,420
+بتحتاج ظروف لا هوائية في النمو فيه بتحتاج ظروف
+
+142
+00:09:48,420 --> 00:09:51,580
+هوائية لو أنا البكتيريا بتحتاج ظروف هوائية في الـ
+
+143
+00:09:51,580 --> 00:09:54,980
+Incubator العادى هيك بيوفرلي ظروف هوائية وظروف 37
+
+144
+00:09:54,980 --> 00:09:59,570
+مناسبة للنمولكن لو البكتيريا لا هوائية الـ auto
+
+145
+00:09:59,570 --> 00:10:02,550
+incubator ما بنمي high البكتيريا فبالتالي أنا
+
+146
+00:10:02,550 --> 00:10:07,060
+بحتاج إلى الـ anaerobic jarهذا بيوفر ليه الظروف
+
+147
+00:10:07,060 --> 00:10:11,020
+اللاهوائية هو عبارة عن مرتبان ججاجي بيكون محكم
+
+148
+00:10:11,020 --> 00:10:14,260
+الإغلاق موجود فيه case هذا ال case فيه مواد
+
+149
+00:10:14,260 --> 00:10:19,800
+كيميائية من جوا بالنهاية بتكون نواتج هذه النواتج
+
+150
+00:10:19,800 --> 00:10:23,800
+عبارة عن بتوفر ظروف لاهوائية نتعرف عليها بالتفصيل
+
+151
+00:10:23,800 --> 00:10:28,940
+في معمل الـ Bacterial Oxygen Requirement بالتالي
+
+152
+00:10:28,940 --> 00:10:32,340
+أخر شهر يتكون ظروف لاهوائية مناسبة لنمو البكتيريا
+
+153
+00:10:32,680 --> 00:10:38,100
+بعد ما أنا طبعا أسكر هذا أحط الأطباق البكتيرية
+
+154
+00:10:38,100 --> 00:10:44,040
+اللي أنا بحتاجها حتى تنمو و بسكر المرتبان بحطها في
+
+155
+00:10:44,040 --> 00:10:48,380
+الـ incubator العادي هيك أنا بتكون موفرة ضروف 37 و
+
+156
+00:10:48,380 --> 00:10:52,360
+ظروف لا هوائية ال candle jar من اسمها candle شمعة
+
+157
+00:10:52,360 --> 00:10:57,020
+لأن أنا بستخدم شمعة في هذا ال candle في بكتيريا
+
+158
+00:10:57,020 --> 00:11:01,790
+حتى تنمو تحت ظروف الـ CO2فحتى أنا أوفر ظروف الـ
+
+159
+00:11:01,790 --> 00:11:07,470
+CO2 بجيب جار محكم الإغلاقهذا الـ Jar بتكون من
+
+160
+00:11:07,470 --> 00:11:11,430
+أطباق بكتيريا بحط الأطباق اللي أنا بدي أعزل
+
+161
+00:11:11,430 --> 00:11:16,950
+البكتيريا اللي بتحتاج CO2 و بثبت أخر شيء slide حتى
+
+162
+00:11:16,950 --> 00:11:20,750
+أحط slide حتى أثبت عليها الشامعة و بضو الشامعة و
+
+163
+00:11:20,750 --> 00:11:26,090
+بسكر هذا المرتبان لما تطف الشامعة هك أنا بتأكد أنه
+
+164
+00:11:26,090 --> 00:11:29,770
+والله اتوفر الظروف CO2 بحمل هذا الـ Jar و بحطه في
+
+165
+00:11:29,770 --> 00:11:34,270
+ال incubator العادي هك أنا اتوفرت اندي ظروف 37الـ
+
+166
+00:11:34,270 --> 00:11:38,730
+CO2 اللازم ينمو البكتيريا بنسين بارنر اللي هو لهاب
+
+167
+00:11:38,730 --> 00:11:43,410
+بنسين لهاب بنسين ضروري جدا لحتى انه خلال شغلي انا
+
+168
+00:11:43,410 --> 00:11:47,330
+في ال micro لازم يكون اللهاب مشغل حتى اني انا اوفر
+
+169
+00:11:47,330 --> 00:11:50,770
+ظروف معقمة امنع ال contamination باستخدم برضه
+
+170
+00:11:50,770 --> 00:11:54,070
+اللهاب في تعقيم الـ necrum lowالـ Loop ها هي
+
+171
+00:11:54,070 --> 00:11:57,550
+الأداة اللي أنا بستخدمها في زراعة البكتيريا في
+
+172
+00:11:57,550 --> 00:12:01,210
+اندينيكروم Loop هذا مش disposable ممكن أنا أستخدمه
+
+173
+00:12:01,210 --> 00:12:05,270
+أكتر من مرة ممكن أعقمه، بعقمه أو لازم أني أنا
+
+174
+00:12:05,270 --> 00:12:09,730
+أعقمه باللهب بنسان بحطه على اللهب لما يتوهج و يصير
+
+175
+00:12:09,730 --> 00:12:14,290
+لونه أحمر، هيك بعرف أنه صار استرال، صار معقم ممكن
+
+176
+00:12:14,290 --> 00:12:18,090
+أستخدمه مرة أخرىفي عندي plastic loop هاي بتكون
+
+177
+00:12:18,090 --> 00:12:21,730
+disposable فقط مرة واحدة فقط مرة واحدة أنا
+
+178
+00:12:21,730 --> 00:12:25,070
+بستخدمها بزرع بكتيريا بعد ما أتخلص من زراعة
+
+179
+00:12:25,070 --> 00:12:28,690
+البكتيريا بحطها في الـ Autoclave pack وما بستخدمها
+
+180
+00:12:28,690 --> 00:12:33,550
+مرة تانية بالنسبة لالأنا Aerobic Station هي عبارة
+
+181
+00:12:33,550 --> 00:12:38,310
+عن محطة بتوفر ظروف لا هوائية فيها أن ممكن أنا أدخل
+
+182
+00:12:38,310 --> 00:12:42,640
+إيديا من خلال هاي ممكن أنا أقدر أشتغلوهي بتوفر
+
+183
+00:12:42,640 --> 00:12:46,600
+لظروف لاهوائية Safety Cabinet بتوفر الأمان و
+
+184
+00:12:46,600 --> 00:12:52,640
+الحماية ومن خلالها أنا بكون شغلي كله بمنع ال
+
+185
+00:12:52,640 --> 00:12:57,580
+contamination بتتعقم من خلال ال UV في UV طبعا بكون
+
+186
+00:12:57,580 --> 00:13:01,100
+موجود في هذا Safety Cabinet بتعقم كل المكان اللي
+
+187
+00:13:01,100 --> 00:13:03,840
+أنا بشتغل فيه وهيك بضمن أن والله مافيه عندي أي
+
+188
+00:13:03,840 --> 00:13:06,780
+contamination هاي بالنسبة للأدوات اللي احنا
+
+189
+00:13:06,780 --> 00:13:10,630
+بنستعملها في معمن الـ Microbiologyطبعاً في أدوات
+
+190
+00:13:10,630 --> 00:13:13,310
+هنتعرف عليها في التفصيل كيف قاليه يتعملها في
+
+191
+00:13:13,310 --> 00:13:17,650
+المقشبات الأخرى و هنتعرف على الأدوات ان شاء الله
+
+192
+00:13:17,650 --> 00:13:21,950
+لما نشتغل العملين ان شاء الله تكون الأمور واضحة
+
+193
+00:13:21,950 --> 00:13:25,250
+انكوا هذا بالنسبة لأول chapter في معمل ال
+
+194
+00:13:25,250 --> 00:13:28,530
+microbiology والسلام عليكم ورحمة الله وبركاته
+
diff --git a/PL9fwy3NUQKwZWgAb_V1ATlRRsH-6i3cPC/lhmDRi5HdZw_raw.json b/PL9fwy3NUQKwZWgAb_V1ATlRRsH-6i3cPC/lhmDRi5HdZw_raw.json
new file mode 100644
index 0000000000000000000000000000000000000000..6655bd95dc5f554d86cdc139b61ee863b15338cb
--- /dev/null
+++ b/PL9fwy3NUQKwZWgAb_V1ATlRRsH-6i3cPC/lhmDRi5HdZw_raw.json
@@ -0,0 +1 @@
+{"segments": [{"id": 1, "seek": 2642, "start": 0.5, "end": 26.42, "text": "أتكلمنا سابقًا عن الـ Spells والانثكابات وقلنا لو صار عندي أي انثكاب أو أي انكسار بخبر ال instructor عشان يعطيني التعليمات في التعامل معها هنتكلم في هاي ال slide كيف أنا بدي أتعامل مع ال Spells دي contamination of spells of small or moderate spells مع الانثكاب اللي بكون صغير ومتوسط لأنه أنا فيه عندي", "tokens": [10721, 2655, 4117, 19528, 8315, 8608, 16758, 4587, 14111, 995, 18871, 2423, 39184, 3550, 43909, 16070, 7649, 12984, 4117, 16758, 9307, 4032, 4587, 1211, 8315, 45164, 20328, 9640, 18871, 16254, 36632, 16472, 12984, 4117, 16758, 34051, 36632, 16472, 4117, 3794, 9640, 4724, 9778, 26890, 2423, 18499, 6225, 8592, 7649, 7251, 3615, 9566, 9957, 1829, 16712, 3615, 20292, 2304, 9307, 8978, 16712, 3615, 10943, 1211, 20449, 11296, 8032, 29399, 4117, 19528, 8978, 8032, 47302, 2423, 4137, 9122, 33911, 41850, 4724, 16254, 5551, 2655, 3615, 10943, 1211, 20449, 2423, 3550, 43909, 11778, 1829, 33012, 295, 25053, 295, 1359, 420, 18174, 25053, 20449, 2423, 7649, 12984, 4117, 16758, 13672, 1829, 4724, 30544, 20328, 17082, 13546, 4032, 2304, 2655, 41779, 9566, 5296, 33456, 3224, 41850, 8978, 3224, 18871, 16254], "avg_logprob": -0.20076884400276912, "compression_ratio": 1.7992424242424243, "no_speech_prob": 4.410743713378906e-06, "words": [{"start": 0.5, "end": 0.96, "word": "أتكلمنا", "probability": 0.83330078125}, {"start": 0.96, "end": 1.52, "word": " سابقًا", "probability": 0.85390625}, {"start": 1.52, "end": 1.8, "word": " عن", "probability": 0.97705078125}, {"start": 1.8, "end": 2.0, "word": " الـ", "probability": 0.851806640625}, {"start": 2.0, "end": 2.28, "word": " Spells", "probability": 0.57666015625}, {"start": 2.28, "end": 3.24, "word": " والانثكابات", "probability": 0.7010904947916666}, {"start": 3.24, "end": 3.58, "word": " وقلنا", "probability": 0.8525390625}, {"start": 3.58, "end": 3.74, "word": " لو", "probability": 0.91162109375}, {"start": 3.74, "end": 3.96, "word": " صار", 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259.96, "word": " ما", "probability": 0.72900390625}, {"start": 259.96, "end": 260.34, "word": " بمسك", "probability": 0.905517578125}, {"start": 260.34, "end": 260.84, "word": " الممسحة", "probability": 0.857373046875}, {"start": 260.84, "end": 260.9, "word": " و", "probability": 0.826171875}, {"start": 260.9, "end": 261.3, "word": " بحطها", "probability": 0.97119140625}, {"start": 261.3, "end": 261.4, "word": " و", "probability": 0.9443359375}, {"start": 261.4, "end": 261.72, "word": " برميها", "probability": 0.95390625}, {"start": 261.72, "end": 261.84, "word": " في", "probability": 0.9453125}, {"start": 261.84, "end": 261.96, "word": " أي", "probability": 0.7451171875}, {"start": 261.96, "end": 262.3, "word": " مكان", "probability": 0.996337890625}, {"start": 262.3, "end": 262.88, "word": " لأ", "probability": 0.9599609375}, {"start": 262.88, "end": 263.6, "word": " بقولك", "probability": 0.9060872395833334}, {"start": 263.6, "end": 264.14, "word": " ضروري", "probability": 0.9273681640625}, {"start": 264.14, "end": 264.3, "word": " دي", "probability": 0.84716796875}, {"start": 264.3, "end": 264.44, "word": " و", "probability": 0.46728515625}, {"start": 264.44, "end": 264.96, "word": " contaminated", "probability": 0.260498046875}], "temperature": 1.0}, {"id": 11, "seek": 29445, "start": 267.34, "end": 294.46, "text": "بنحطها في مطهر عشان ما يكون فيها بكتيريا wipe area with disinfectant and clean paper towel and pot in biohazard bag بعد ما أخلص بغطي المنطقة بإيتيشو بابر بمطهر ومعقم وبعد ما أخلص بحط هذه الأدوات اللي استخدمتها في ال biohazard bag ال biohazard bag هي نفسها ال auto-clav bag لأن إيتيشو بابر مش إشيحات فبحطه في ال auto-clav bag", "tokens": [3555, 1863, 5016, 9566, 11296, 8978, 3714, 9566, 3224, 2288, 6225, 8592, 7649, 19446, 7251, 30544, 8978, 11296, 4724, 4117, 2655, 13546, 25528, 14082, 1859, 365, 33334, 394, 293, 2541, 3035, 15755, 293, 1847, 294, 12198, 71, 921, 515, 3411, 39182, 19446, 5551, 9778, 1211, 9381, 4724, 17082, 9566, 1829, 9673, 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" عشان", "probability": 0.9358723958333334}, {"start": 269.12, "end": 269.28, "word": " ما", "probability": 0.97021484375}, {"start": 269.28, "end": 269.64, "word": " يكون", "probability": 0.9169921875}, {"start": 269.64, "end": 270.12, "word": " فيها", "probability": 0.97314453125}, {"start": 270.12, "end": 270.88, "word": " بكتيريا", "probability": 0.955078125}, {"start": 270.88, "end": 271.82, "word": " wipe", "probability": 0.165771484375}, {"start": 271.82, "end": 272.28, "word": " area", "probability": 0.84423828125}, {"start": 272.28, "end": 272.48, "word": " with", "probability": 0.830078125}, {"start": 272.48, "end": 273.3, "word": " disinfectant", "probability": 0.9306640625}, {"start": 273.3, "end": 273.54, "word": " and", "probability": 0.94775390625}, {"start": 273.54, "end": 273.9, "word": " clean", "probability": 0.85986328125}, {"start": 273.9, "end": 274.22, "word": " paper", "probability": 0.94189453125}, {"start": 274.22, "end": 274.64, "word": " towel", "probability": 0.11883544921875}, {"start": 274.64, "end": 274.94, "word": " and", "probability": 0.951171875}, {"start": 274.94, "end": 275.2, "word": " pot", "probability": 0.304443359375}, {"start": 275.2, "end": 275.44, "word": " in", "probability": 0.90966796875}, {"start": 275.44, "end": 276.08, "word": " biohazard", "probability": 0.8516845703125}, {"start": 276.08, "end": 276.38, "word": " bag", "probability": 0.94140625}, {"start": 276.38, "end": 276.66, "word": " بعد", "probability": 0.92626953125}, {"start": 276.66, "end": 276.78, "word": " ما", "probability": 0.94091796875}, {"start": 276.78, "end": 277.32, "word": " أخلص", "probability": 0.934814453125}, {"start": 277.32, "end": 278.7, "word": " بغطي", "probability": 0.92529296875}, {"start": 278.7, "end": 279.24, "word": " المنطقة", "probability": 0.9774169921875}, {"start": 279.24, "end": 279.84, "word": " بإيتيشو", "probability": 0.7396443684895834}, {"start": 279.84, "end": 280.26, "word": " بابر", "probability": 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285.6, "end": 286.34, "word": " biohazard", "probability": 0.9310302734375}, {"start": 286.34, "end": 286.62, "word": " bag", "probability": 0.96435546875}, {"start": 286.62, "end": 286.82, "word": " ال", "probability": 0.93505859375}, {"start": 286.82, "end": 287.84, "word": " biohazard", "probability": 0.9803466796875}, {"start": 287.84, "end": 288.24, "word": " bag", "probability": 0.958984375}, {"start": 288.24, "end": 288.46, "word": " هي", "probability": 0.900390625}, {"start": 288.46, "end": 288.94, "word": " نفسها", "probability": 0.9951171875}, {"start": 288.94, "end": 289.12, "word": " ال", "probability": 0.9443359375}, {"start": 289.12, "end": 289.42, "word": " auto", "probability": 0.5615234375}, {"start": 289.42, "end": 290.24, "word": "-clav", "probability": 0.3814290364583333}, {"start": 290.24, "end": 290.56, "word": " bag", "probability": 0.9765625}, {"start": 290.56, "end": 291.2, "word": " لأن", "probability": 0.8203125}, {"start": 291.2, "end": 291.68, "word": " إيتيشو", "probability": 0.873974609375}, {"start": 291.68, "end": 292.0, "word": " بابر", "probability": 0.99462890625}, {"start": 292.0, "end": 292.18, "word": " مش", "probability": 0.98388671875}, {"start": 292.18, "end": 292.7, "word": " إشيحات", "probability": 0.7919921875}, {"start": 292.7, "end": 293.16, "word": " فبحطه", "probability": 0.880615234375}, {"start": 293.16, "end": 293.32, "word": " في", "probability": 0.9833984375}, {"start": 293.32, "end": 293.5, "word": " ال", "probability": 0.96484375}, {"start": 293.5, "end": 293.74, "word": " auto", "probability": 0.86083984375}, {"start": 293.74, "end": 294.08, "word": "-clav", "probability": 0.9480794270833334}, {"start": 294.08, "end": 294.46, "word": " bag", "probability": 0.97265625}], "temperature": 1.0}, {"id": 12, "seek": 31697, "start": 295.09, "end": 316.97, "text": "move if spill is in floor طبعا بمسح المنطقة أو بمسح الأرضية لو كان ال spill على الأرضية remove gloves and shoes cover before removing area of the spill بقولك قبل ما تطلع من منطقة الانسكاب بتتخلصي من ال gloves و من الغطاء الحذاء و بتحطي في ال bio hazard", "tokens": [76, 1682, 498, 22044, 307, 294, 4123, 23032, 3555, 3615, 995, 4724, 2304, 3794, 5016, 9673, 1863, 9566, 28671, 34051, 4724, 2304, 3794, 5016, 16247, 43042, 10632, 45164, 25961, 2423, 22044, 15844, 16247, 43042, 10632, 4159, 14976, 293, 6654, 2060, 949, 12720, 1859, 295, 264, 22044, 4724, 39648, 4117, 12174, 36150, 19446, 6055, 9566, 1211, 3615, 9154, 9154, 9566, 28671, 2423, 7649, 3794, 4117, 16758, 39894, 2655, 9778, 1211, 9381, 1829, 9154, 2423, 14976, 4032, 9154, 6024, 118, 9566, 16606, 21542, 8848, 16606, 4032, 39894, 5016, 9566, 1829, 8978, 2423, 12198, 20790], "avg_logprob": -0.1560819879654915, "compression_ratio": 1.587719298245614, "no_speech_prob": 1.7881393432617188e-06, "words": [{"start": 295.09, "end": 295.53, "word": "move", "probability": 0.598876953125}, {"start": 295.53, "end": 295.77, "word": " if", "probability": 0.97216796875}, {"start": 295.77, "end": 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{"start": 306.81, "end": 307.01, "word": " the", "probability": 0.87890625}, {"start": 307.01, "end": 307.33, "word": " spill", "probability": 0.9794921875}, {"start": 307.33, "end": 307.97, "word": " بقولك", "probability": 0.763427734375}, {"start": 307.97, "end": 308.33, "word": " قبل", "probability": 0.97412109375}, {"start": 308.33, "end": 308.49, "word": " ما", "probability": 0.92041015625}, {"start": 308.49, "end": 309.01, "word": " تطلع", "probability": 0.99072265625}, {"start": 309.01, "end": 309.17, "word": " من", "probability": 0.98486328125}, {"start": 309.17, "end": 309.67, "word": " منطقة", "probability": 0.9874674479166666}, {"start": 309.67, "end": 310.41, "word": " الانسكاب", "probability": 0.8044921875}, {"start": 310.41, "end": 311.35, "word": " بتتخلصي", "probability": 0.9313151041666666}, {"start": 311.35, "end": 311.49, "word": " من", "probability": 0.9970703125}, {"start": 311.49, "end": 311.63, "word": " ال", "probability": 0.74560546875}, {"start": 311.63, 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8592, 2655, 17082, 1211, 18863, 42213, 9957, 16472, 13412, 16606, 21984, 6055, 30544, 16247, 2304, 13063, 4032, 46958, 5016, 3660, 16472, 4117, 14407, 23758, 20666, 1863, 35457, 3660, 5296, 10721, 12610, 7187, 8978, 20449, 42213, 2423, 4532, 5614, 1793, 16070, 3794, 37440, 25894, 24793, 4032, 2288, 35571, 3660, 21984, 4032, 26890, 4117, 9307, 3224], "avg_logprob": -0.15929203328833116, "compression_ratio": 1.7941176470588236, "no_speech_prob": 8.022785186767578e-05, "words": [{"start": 789.51, "end": 789.85, "word": "طبعاً", "probability": 0.880078125}, {"start": 789.85, "end": 789.97, "word": " في", "probability": 0.77294921875}, {"start": 789.97, "end": 790.63, "word": " أدوات", "probability": 0.8763427734375}, {"start": 790.63, "end": 791.05, "word": " هنتعرف", "probability": 0.954833984375}, {"start": 791.05, "end": 791.27, "word": " عليها", "probability": 0.99658203125}, {"start": 791.27, "end": 791.41, "word": " في", "probability": 0.84375}, {"start": 791.41, "end": 791.95, "word": " التفصيل", "probability": 0.8128662109375}, {"start": 791.95, "end": 792.27, "word": " كيف", "probability": 0.944580078125}, {"start": 792.27, "end": 792.57, "word": " قاليه", "probability": 0.51190185546875}, {"start": 792.57, "end": 793.01, "word": " يتعملها", "probability": 0.8650390625}, {"start": 793.01, "end": 793.31, "word": " في", "probability": 0.9716796875}, {"start": 793.31, "end": 794.85, "word": " المقشبات", "probability": 0.8580078125}, {"start": 794.85, "end": 795.53, "word": " الأخرى", "probability": 0.9505208333333334}, {"start": 795.53, "end": 795.99, "word": " و", "probability": 0.68310546875}, {"start": 795.99, "end": 796.53, "word": " هنتعرف", "probability": 0.875244140625}, {"start": 796.53, "end": 796.71, "word": " على", "probability": 0.5400390625}, {"start": 796.71, "end": 797.19, "word": " الأدوات", "probability": 0.98974609375}, {"start": 797.19, "end": 797.33, "word": " ان", "probability": 0.61865234375}, {"start": 797.33, "end": 797.49, "word": " شاء", "probability": 0.991943359375}, {"start": 797.49, "end": 797.65, "word": " الله", "probability": 0.9521484375}, {"start": 797.65, "end": 798.01, "word": " لما", "probability": 0.92578125}, {"start": 798.01, "end": 798.83, "word": " نشتغل", "probability": 0.99599609375}, {"start": 798.83, "end": 799.83, "word": " العملين", "probability": 0.7872721354166666}, {"start": 799.83, "end": 800.29, "word": " ان", "probability": 0.157958984375}, {"start": 800.29, "end": 800.67, "word": " شاء", "probability": 0.98974609375}, {"start": 800.67, "end": 800.75, "word": " الله", "probability": 0.95849609375}, {"start": 800.75, "end": 801.01, "word": " تكون", "probability": 0.966796875}, {"start": 801.01, "end": 801.49, "word": " الأمور", "probability": 0.9710286458333334}, {"start": 801.49, "end": 801.95, "word": " واضحة", "probability": 0.9737548828125}, {"start": 801.95, "end": 802.43, "word": " انكوا", "probability": 0.6671549479166666}, {"start": 802.43, "end": 803.01, "word": " هذا", "probability": 0.84130859375}, {"start": 803.01, "end": 803.49, "word": " بالنسبة", "probability": 0.97998046875}, {"start": 803.49, "end": 803.91, "word": " لأول", "probability": 0.9677734375}, {"start": 803.91, "end": 804.31, "word": " chapter", "probability": 0.8037109375}, {"start": 804.31, "end": 804.63, "word": " في", "probability": 0.9716796875}, {"start": 804.63, "end": 805.05, "word": " معمل", "probability": 0.85498046875}, {"start": 805.05, "end": 805.25, "word": " ال", "probability": 0.84619140625}, {"start": 805.25, "end": 806.17, "word": " microbiology", "probability": 0.6775716145833334}, {"start": 806.17, "end": 806.67, "word": " والسلام", "probability": 0.88037109375}, {"start": 806.67, "end": 807.13, "word": " عليكم", "probability": 0.955810546875}, {"start": 807.13, "end": 807.55, "word": " ورحمة", "probability": 0.9654541015625}, {"start": 807.55, "end": 807.81, "word": " الله", "probability": 0.97314453125}, {"start": 807.81, "end": 808.53, "word": " وبركاته", "probability": 0.98828125}], "temperature": 1.0}], "language": "ar", "language_probability": 1.0, "duration": 810.1906875, "duration_after_vad": 800.230000000001}
\ No newline at end of file
diff --git a/PL9fwy3NUQKwZWgAb_V1ATlRRsH-6i3cPC/lhmDRi5HdZw_raw.srt b/PL9fwy3NUQKwZWgAb_V1ATlRRsH-6i3cPC/lhmDRi5HdZw_raw.srt
new file mode 100644
index 0000000000000000000000000000000000000000..cab0a0fb13a44715ce6d301baa6c8d1bbbee3474
--- /dev/null
+++ b/PL9fwy3NUQKwZWgAb_V1ATlRRsH-6i3cPC/lhmDRi5HdZw_raw.srt
@@ -0,0 +1,776 @@
+1
+00:00:00,500 --> 00:00:03,740
+أتكلمنا سابقًا عن الـ Spells والانثكابات وقلنا لو
+
+2
+00:00:03,740 --> 00:00:08,140
+صار عندي أي انثكاب أو أي انكسار بخبر ال instructor
+
+3
+00:00:08,140 --> 00:00:12,480
+عشان يعطيني التعليمات في التعامل معها هنتكلم في
+
+4
+00:00:12,480 --> 00:00:15,920
+هاي ال slide كيف أنا بدي أتعامل مع ال Spells دي
+
+5
+00:00:15,920 --> 00:00:20,080
+contamination of spells of small or moderate
+
+6
+00:00:20,080 --> 00:00:25,960
+spells مع الانثكاب اللي بكون صغير ومتوسط لأنه أنا
+
+7
+00:00:25,960 --> 00:00:29,990
+فيه عنديممكن يكون large spells انسكاب كبير هيكون
+
+8
+00:00:29,990 --> 00:00:33,330
+في ياندي التعامل مختلف اول حاجة notify other
+
+9
+00:00:33,330 --> 00:00:37,530
+workers in the area of the spells بشعر و بخبر
+
+10
+00:00:37,530 --> 00:00:42,210
+الموجودين في المكان ان والله في إشي انكب في المكان
+
+11
+00:00:42,210 --> 00:00:45,290
+and control the traffic through area و بدي أتحكم
+
+12
+00:00:45,290 --> 00:00:50,290
+في حركة المرور يعني بحكي لأي حد ممنوع انه يقرب على
+
+13
+00:00:50,290 --> 00:00:55,770
+هذه المنطقة لإنه والله صار في ياندي انسكابshoe
+
+14
+00:00:55,770 --> 00:00:59,130
+cover and safety goggles بقولك بتلبسي ال shoe
+
+15
+00:00:59,130 --> 00:01:03,030
+covers نلبس الغطاء الأحذية ليش لأنه أنا خلال ال
+
+16
+00:01:03,030 --> 00:01:06,850
+handicap ممكن يكون والله صار في عندي touch بين
+
+17
+00:01:06,850 --> 00:01:11,730
+بكتيريا وهي البكتيريا تكون معدية تمام فبالتالي حتى
+
+18
+00:01:11,730 --> 00:01:17,470
+أمنع نقل العدو لكل مكان بروحه وكمان لحذائي ل ال
+
+19
+00:01:17,470 --> 00:01:21,750
+shoes تبعي بستخدم اللي هو ال shoe cover and safety
+
+20
+00:01:21,750 --> 00:01:25,850
+goggles اللي هي النضارات لأنه ممكن يكوننتيجة
+
+21
+00:01:25,850 --> 00:01:29,190
+الانسكاب صار في عندي رداد في الجو وبالتالي يدخل في
+
+22
+00:01:29,190 --> 00:01:33,210
+عيني ويسببلي infection if a spill is on a floor
+
+23
+00:01:33,210 --> 00:01:36,350
+may be splashed beyond immediate area of the spill
+
+24
+00:01:36,350 --> 00:01:40,450
+بقولك ممكن ال spill ينتشر كمان للمنطقة المجاورة
+
+25
+00:01:40,450 --> 00:01:44,330
+put on gloves بقولك بعدها تلبسي ل gloves and cover
+
+26
+00:01:44,330 --> 00:01:49,030
+spill area with paper towel بغطي منطقة الانسكاب
+
+27
+00:01:49,030 --> 00:01:54,880
+بتشوبابربعدها بور disinfectant over towel from
+
+28
+00:01:54,880 --> 00:02:00,220
+each of the spill to center بغطي او بحاول ا skip
+
+29
+00:02:00,220 --> 00:02:05,100
+disinfectant اللي هو ال detail متهر على اللي هو ال
+
+30
+00:02:05,100 --> 00:02:09,880
+tissue paper اللي انا حطيتهم وبحاول اخد من ال each
+
+31
+00:02:10,270 --> 00:02:15,370
+من الحواف حتى المركز ليش حتى أني أمنع انتشار اللي
+
+32
+00:02:15,370 --> 00:02:20,850
+هو الانتشار الانسكاب لأني لو جبت من المركز لبرا
+
+33
+00:02:20,850 --> 00:02:24,050
+معناه الكلام أني أنا جبت بكتيريا ووزعتها وصارت
+
+34
+00:02:24,050 --> 00:02:28,410
+الدائرة أكبر بقولك كل الأدوات دي كونتمانات all
+
+35
+00:02:28,410 --> 00:02:32,830
+object in spell area كل الأدوات اللي موجودة في
+
+36
+00:02:32,830 --> 00:02:37,140
+منطقة الانسكاب بحاول أني أنا أعقمها و أنظفهالأنه
+
+37
+00:02:37,140 --> 00:02:41,940
+ممكن يكون الرذاذ إيجا عليها allow بس تنتظر لمدة 30
+
+38
+00:02:41,940 --> 00:02:46,380
+دقيقة of contact time حتى هذه 30 دقيقة تكون كافية
+
+39
+00:02:46,380 --> 00:02:54,160
+حتى إنه يتم قتل كل شي Infectious، تمام؟Pick
+
+40
+00:02:54,160 --> 00:02:58,620
+up any charp including broken glass with forceps
+
+41
+00:02:58,620 --> 00:03:02,220
+and place in char container بقولك لو في أي مواد
+
+42
+00:03:02,220 --> 00:03:05,960
+حادة أو مواد زجاجية نتيجة الانكسار أو الانسكاب
+
+43
+00:03:06,300 --> 00:03:09,800
+بحاول أني ألتقطه بالـ forceps الـ forceps اللي هو
+
+44
+00:03:09,800 --> 00:03:13,620
+الملقط ممنوع أنا أستخدم إيدي لأنه ممكن يصير في
+
+45
+00:03:13,620 --> 00:03:18,560
+عندي infection و يصير systemic infection و تدخل
+
+46
+00:03:18,560 --> 00:03:22,320
+الدم و يسبب لي طبعا أمراض عندي place in a charred
+
+47
+00:03:22,320 --> 00:03:26,200
+container بعد ما هذا أنا بحط هذه المواد الحادة و
+
+48
+00:03:26,200 --> 00:03:29,120
+ال broken glass في ال charred container وشو هو ال
+
+49
+00:03:29,120 --> 00:03:32,660
+charred container هو نفسه ال puncture resistant
+
+50
+00:03:32,660 --> 00:03:35,890
+container اللي اتكلمنا عنه اللي قلناأي مواد حد أنا
+
+51
+00:03:35,890 --> 00:03:40,930
+بستخدمها اللي هي الملقط المشروط كلها هاي بحطها بيه
+
+52
+00:03:40,930 --> 00:03:45,130
+puncture resistant container container مقاول للخرق
+
+53
+00:03:45,130 --> 00:03:48,310
+لأنه انا لو حطيتها في autoclave bag هذا ال case
+
+54
+00:03:48,310 --> 00:03:52,590
+ممكن يخترق ال case ال needle مثلا المواد الحد ده
+
+55
+00:03:52,590 --> 00:03:56,350
+ممكن تخترق ال case وبالتالي تسببلي الأداء و تعملي
+
+56
+00:03:56,350 --> 00:04:00,960
+infectionUse a sewage and dust pan بقولك هان
+
+57
+00:04:00,960 --> 00:04:05,120
+استخدمي اللي هي معناه هاي ال sewage and dust pan
+
+58
+00:04:05,120 --> 00:04:10,340
+هي الممسحة to recover any charred of broken glass
+
+59
+00:04:10,340 --> 00:04:15,100
+in contaminated liquid نستخدم الممسح حتى نتخلص من
+
+60
+00:04:15,100 --> 00:04:19,960
+أي شضايا من الزجاج بعد ما أخلص من الممسح طبعا ما
+
+61
+00:04:19,960 --> 00:04:23,600
+بمسك الممسحة و بحطها و برميها في أي مكان لأ بقولك
+
+62
+00:04:23,600 --> 00:04:29,640
+ضروري دي و contaminatedبنحطها في مطهر عشان ما يكون
+
+63
+00:04:29,640 --> 00:04:33,900
+فيها بكتيريا wipe area with disinfectant and clean
+
+64
+00:04:33,900 --> 00:04:37,320
+paper towel and pot in biohazard bag بعد ما أخلص
+
+65
+00:04:37,320 --> 00:04:43,200
+بغطي المنطقة بإيتيشو بابر بمطهر ومعقم وبعد ما أخلص
+
+66
+00:04:43,200 --> 00:04:46,620
+بحط هذه الأدوات اللي استخدمتها في ال biohazard bag
+
+67
+00:04:46,620 --> 00:04:51,200
+ال biohazard bag هي نفسها ال auto-clav bag لأن
+
+68
+00:04:51,200 --> 00:04:54,460
+إيتيشو بابر مش إشيحات فبحطه في ال auto-clav bag
+
+69
+00:04:55,090 --> 00:04:59,750
+move if spill is in floor طبعا بمسح المنطقة أو
+
+70
+00:04:59,750 --> 00:05:03,370
+بمسح الأرضية لو كان ال spill على الأرضية remove
+
+71
+00:05:03,370 --> 00:05:07,010
+gloves and shoes cover before removing area of the
+
+72
+00:05:07,010 --> 00:05:11,350
+spill بقولك قبل ما تطلع من منطقة الانسكاب بتتخلصي
+
+73
+00:05:11,350 --> 00:05:16,550
+من ال gloves و من الغطاء الحذاء و بتحطي في ال bio
+
+74
+00:05:16,550 --> 00:05:20,830
+hazardاللي هو الـ Autoclave bag حتى اني انا امنع
+
+75
+00:05:20,830 --> 00:05:25,810
+انتشار طبعا اللي هو العدوى بعد ما اخلص بغسل ايدي
+
+76
+00:05:25,810 --> 00:05:30,250
+بالميه والصابون هذا بالنسبة لـ Small and Moderate
+
+77
+00:05:30,250 --> 00:05:34,510
+Spills اما بالنسبة لـ Large Spill الـ Large Spill
+
+78
+00:05:34,510 --> 00:05:39,670
+بقولك evacuate room بدك تخلي الغرفة close door بدك
+
+79
+00:05:39,670 --> 00:05:43,560
+تسكري البوابand prevent others from entering و
+
+80
+00:05:43,560 --> 00:05:47,700
+بديك تمنعي أدح أي حد أنه يدخل هذه الغرفة and where
+
+81
+00:05:47,700 --> 00:05:52,840
+بديك تنتظر 30 دقيقة حتى أنه الرذاذ يستقر على الأرض
+
+82
+00:05:52,840 --> 00:05:57,460
+و الأجهزة عشان أنا لما أدخل مايكون إشي موجود فيه
+
+83
+00:05:57,460 --> 00:06:00,660
+الهواء و أنا أستنشقه و يقلقش المخاطرية و يسببلي
+
+84
+00:06:00,660 --> 00:06:05,230
+infectionمش أستنى تلاتين في ال .. اتكلمنا احنا في
+
+85
+00:06:05,230 --> 00:06:08,530
+الـ small و في ال moderate spells تلاتين دقيقة حتى
+
+86
+00:06:08,530 --> 00:06:13,150
+تكون كافية لقتل ال .. اللي هو البكتيريا لكن هان
+
+87
+00:06:13,150 --> 00:06:16,950
+بدي تلاتين دقيقة حتى اني انا اضمن انه ال radar
+
+88
+00:06:16,950 --> 00:06:21,610
+استقر على الأرض و على الأجهزة بعدها باتبع نفس
+
+89
+00:06:21,610 --> 00:06:25,550
+الخطوات اللي انااستخدمتها في الـ small و ال
+
+90
+00:06:25,550 --> 00:06:29,030
+moderate spells بما فيهم اخر شئ اني انا انتظر كمان
+
+91
+00:06:29,030 --> 00:06:33,990
+تلاتين دقيقة حتى انها تكون كافية انها تقتل كل
+
+92
+00:06:33,990 --> 00:06:38,030
+البكتيريا هذه بعض الملاحظات بالنسبة ل .. بالمعمل
+
+93
+00:06:38,030 --> 00:06:42,230
+ان ممنوح حد يدخل المعمل بدون اي حد من الاساتذة
+
+94
+00:06:42,650 --> 00:06:46,770
+الممنوع أنه أنا الأدوات الشخصية أني أحطها في منطقة
+
+95
+00:06:46,770 --> 00:06:49,730
+الشغل جوالات ممنوع الجوالات في منطقة الشغل ممنوع
+
+96
+00:06:49,730 --> 00:06:53,230
+الأدوات الشخصية من لو أنا اضطرت أني أنا أستخدم أي
+
+97
+00:06:53,230 --> 00:06:58,800
+قلمدفتر بعد ما أخلص أحاول أني أنا أعقمهم كل شعب
+
+98
+00:06:58,800 --> 00:07:02,920
+هتتقسم لجروبات كل جروب هيكون طبعا متكون من أربع
+
+99
+00:07:02,920 --> 00:07:08,060
+بنات كل هدول الأربع بنات أو هدول الجروب هيدلوا طول
+
+100
+00:07:08,060 --> 00:07:12,040
+الفصل يشتغل نفس الشغل و يكتب حتى أنه يكون النتاج
+
+101
+00:07:12,040 --> 00:07:16,060
+التقارير تكون نفسها بالنسبة للأشياء المحتاجة في
+
+102
+00:07:16,060 --> 00:07:19,820
+المعمل ال lab code ضروري جدا جداالـ Match أو الـ
+
+103
+00:07:19,820 --> 00:07:22,520
+Lighter الأداحة أنا أكيد معملة الـ Microbiology
+
+104
+00:07:22,520 --> 00:07:25,700
+هنستخدم اللها فبالتالي ضروري كل group يكون معاه
+
+105
+00:07:25,700 --> 00:07:29,520
+أداحة الصابونة بدنا قولنا عن ال safety rules بدنا
+
+106
+00:07:29,520 --> 00:07:33,340
+نغسل أديانا قبل و بعد الشغل بميه والصابون الماركر
+
+107
+00:07:33,340 --> 00:07:37,020
+ضروري برضه كل group يكون معاه ماركر Small أو
+
+108
+00:07:37,020 --> 00:07:40,780
+Medium Size دوال منشفة تكون صغيرة حتى أني أنا بعد
+
+109
+00:07:40,780 --> 00:07:46,060
+ما أغسل أديا أنشفهمساليابول بلاستيك باك هى انه
+
+110
+00:07:46,060 --> 00:07:49,100
+هتكون حقيبة حتى اني انا احط فيها ال lab coat ممكن
+
+111
+00:07:49,100 --> 00:07:51,980
+كيس انك تحط فيه ال lab coat عشان تفصليه عن أدواتك
+
+112
+00:07:51,980 --> 00:07:55,360
+التانية و ما يصف يد فيه اننا infection rubber band
+
+113
+00:07:55,360 --> 00:07:59,040
+اللى هى مطاط المطاطة هى ممكن انا استخدمها في حال
+
+114
+00:07:59,040 --> 00:08:03,560
+كان ال lab coat اللى يكون الاديان واسعين فهيك ممكن
+
+115
+00:08:03,560 --> 00:08:07,740
+يعيق الشغل فانا ممكن احط ال rubber band حتى تقدر
+
+116
+00:08:07,740 --> 00:08:12,250
+تشتغلي بكل سهولةGeneral Microbiology Manual هذا
+
+117
+00:08:12,250 --> 00:08:16,550
+الكتاب الملزمة اللي حكينا عنها هي الملزمة نزلناها
+
+118
+00:08:16,550 --> 00:08:22,830
+على الـ Model موجودة ان شاء الله لما نشتغل الشغل
+
+119
+00:08:22,830 --> 00:08:26,150
+لما نبدأ بالشغل العملي هنتكلم عنها لأنه فيها هيكون
+
+120
+00:08:26,150 --> 00:08:30,770
+كتابة تقارير بالنسبة لـ Instrument in Microbiology
+
+121
+00:08:30,770 --> 00:08:33,650
+Lab الأدوات اللي أنا بستخدمها في معمل الـ
+
+122
+00:08:33,650 --> 00:08:37,420
+Microbiologyأول أداة اللي هي ال incubator ال
+
+123
+00:08:37,420 --> 00:08:42,600
+incubator هو عبارة عن حاضنة معناها الحاضنة ال
+
+124
+00:08:42,600 --> 00:08:46,480
+incubator هي عبارة عن جهاز بوفر لي ظروف مناسبة
+
+125
+00:08:46,480 --> 00:08:49,140
+لنمو البكتيريا اللي بقى لو بدي أعرف ال incubator
+
+126
+00:08:49,140 --> 00:08:53,540
+بحكي device which confirm appropriate condition
+
+127
+00:08:53,540 --> 00:08:57,180
+for bacterial growth بيوفر ظروف مناسبة لنمو
+
+128
+00:08:57,180 --> 00:08:59,760
+البكتيريا غالبا درجة الحرارة اللي بتنمو عليها
+
+129
+00:08:59,760 --> 00:09:02,520
+البكتيريا سبعة و تلاتين هن بيكون مكتوب درجة
+
+130
+00:09:02,520 --> 00:09:06,640
+الحرارة سبعة و تلاتينلما اشوف 37 هك انا مضمن ان
+
+131
+00:09:06,640 --> 00:09:10,280
+والله في عندي 37 مناسبة لنمو البكتيريا بنحط
+
+132
+00:09:10,280 --> 00:09:14,160
+الأطباق اللي بتردش هنا وبعدين بنسكر بعد 24 ساعة
+
+133
+00:09:14,160 --> 00:09:17,300
+بنلاحظ نمو البكتيريا طبعا هذا ال incubator يكون
+
+134
+00:09:17,300 --> 00:09:22,460
+يوفر ظروف هوائية ال auto-clef هو عبارة عن جهاز
+
+135
+00:09:22,460 --> 00:09:27,320
+بنستخدمه used for sterilization لا تعقيمزي تنجرة
+
+136
+00:09:27,320 --> 00:09:30,980
+الضغط إذا ملاحظين بيوفرلي درجة حرارة عالية وضغط
+
+137
+00:09:30,980 --> 00:09:35,340
+عالي كافي إنه يقتللي all microorganism or all
+
+138
+00:09:35,340 --> 00:09:39,600
+بكتيريا يعني يقتللي كل البكتيريا بالنسبالي الـ
+
+139
+00:09:39,600 --> 00:09:42,220
+Anaerobic Jar و فيه اندي الـ Candle Jar احنا قولنا
+
+140
+00:09:42,220 --> 00:09:45,560
+فيه بكتيريا طبعا بتختلف في خصائصها فيه بكتيريا
+
+141
+00:09:45,560 --> 00:09:48,420
+بتحتاج ظروف لا هوائية في النمو فيه بتحتاج ظروف
+
+142
+00:09:48,420 --> 00:09:51,580
+هوائية لو أنا البكتيريا بتحتاج ظروف هوائية في الـ
+
+143
+00:09:51,580 --> 00:09:54,980
+Incubator العادى هيك بيوفرلي ظروف هوائية وظروف 37
+
+144
+00:09:54,980 --> 00:09:59,570
+مناسبة للنمولكن لو البكتيريا لا هوائية الـ auto
+
+145
+00:09:59,570 --> 00:10:02,550
+incubator ما بنمي high البكتيريا فبالتالي أنا
+
+146
+00:10:02,550 --> 00:10:07,060
+بحتاج إلى الـ anaerobic jarهذا بيوفر ليه الظروف
+
+147
+00:10:07,060 --> 00:10:11,020
+اللاهوائية هو عبارة عن مرتبان ججاجي بيكون محكم
+
+148
+00:10:11,020 --> 00:10:14,260
+الإغلاق موجود فيه case هذا ال case فيه مواد
+
+149
+00:10:14,260 --> 00:10:19,800
+كيميائية من جوا بالنهاية بتكون نواتج هذه النواتج
+
+150
+00:10:19,800 --> 00:10:23,800
+عبارة عن بتوفر ظروف لاهوائية نتعرف عليها بالتفصيل
+
+151
+00:10:23,800 --> 00:10:28,940
+في معمل الـ Bacterial Oxygen Requirement بالتالي
+
+152
+00:10:28,940 --> 00:10:32,340
+أخر شهر يتكون ظروف لاهوائية مناسبة لنمو البكتيريا
+
+153
+00:10:32,680 --> 00:10:38,100
+بعد ما أنا طبعا أسكر هذا أحط الأطباق البكتيرية
+
+154
+00:10:38,100 --> 00:10:44,040
+اللي أنا بحتاجها حتى تنمو و بسكر المرتبان بحطها في
+
+155
+00:10:44,040 --> 00:10:48,380
+الـ incubator العادي هيك أنا بتكون موفرة ضروف 37 و
+
+156
+00:10:48,380 --> 00:10:52,360
+ظروف لا هوائية ال candle jar من اسمها candle شمعة
+
+157
+00:10:52,360 --> 00:10:57,020
+لأن أنا بستخدم شمعة في هذا ال candle في بكتيريا
+
+158
+00:10:57,020 --> 00:11:01,790
+حتى تنمو تحت ظروف الـ CO2فحتى أنا أوفر ظروف الـ
+
+159
+00:11:01,790 --> 00:11:07,470
+CO2 بجيب جار محكم الإغلاقهذا الـ Jar بتكون من
+
+160
+00:11:07,470 --> 00:11:11,430
+أطباق بكتيريا بحط الأطباق اللي أنا بدي أعزل
+
+161
+00:11:11,430 --> 00:11:16,950
+البكتيريا اللي بتحتاج CO2 و بثبت أخر شيء slide حتى
+
+162
+00:11:16,950 --> 00:11:20,750
+أحط slide حتى أثبت عليها الشامعة و بضو الشامعة و
+
+163
+00:11:20,750 --> 00:11:26,090
+بسكر هذا المرتبان لما تطف الشامعة هك أنا بتأكد أنه
+
+164
+00:11:26,090 --> 00:11:29,770
+والله اتوفر الظروف CO2 بحمل هذا الـ Jar و بحطه في
+
+165
+00:11:29,770 --> 00:11:34,270
+ال incubator العادي هك أنا اتوفرت اندي ظروف 37الـ
+
+166
+00:11:34,270 --> 00:11:38,730
+CO2 اللازم ينمو البكتيريا بنسين بارنر اللي هو لهاب
+
+167
+00:11:38,730 --> 00:11:43,410
+بنسين لهاب بنسين ضروري جدا لحتى انه خلال شغلي انا
+
+168
+00:11:43,410 --> 00:11:47,330
+في ال micro لازم يكون اللهاب مشغل حتى اني انا اوفر
+
+169
+00:11:47,330 --> 00:11:50,770
+ظروف معقمة امنع ال contamination باستخدم برضه
+
+170
+00:11:50,770 --> 00:11:54,070
+اللهاب في تعقيم الـ necrum lowالـ Loop ها هي
+
+171
+00:11:54,070 --> 00:11:57,550
+الأداة اللي أنا بستخدمها في زراعة البكتيريا في
+
+172
+00:11:57,550 --> 00:12:01,210
+اندينيكروم Loop هذا مش disposable ممكن أنا أستخدمه
+
+173
+00:12:01,210 --> 00:12:05,270
+أكتر من مرة ممكن أعقمه، بعقمه أو لازم أني أنا
+
+174
+00:12:05,270 --> 00:12:09,730
+أعقمه باللهب بنسان بحطه على اللهب لما يتوهج و يصير
+
+175
+00:12:09,730 --> 00:12:14,290
+لونه أحمر، هيك بعرف أنه صار استرال، صار معقم ممكن
+
+176
+00:12:14,290 --> 00:12:18,090
+أستخدمه مرة أخرىفي عندي plastic loop هاي بتكون
+
+177
+00:12:18,090 --> 00:12:21,730
+disposable فقط مرة واحدة فقط مرة واحدة أنا
+
+178
+00:12:21,730 --> 00:12:25,070
+بستخدمها بزرع بكتيريا بعد ما أتخلص من زراعة
+
+179
+00:12:25,070 --> 00:12:28,690
+البكتيريا بحطها في الـ Autoclave pack وما بستخدمها
+
+180
+00:12:28,690 --> 00:12:33,550
+مرة تانية بالنسبة لالأنا Aerobic Station هي عبارة
+
+181
+00:12:33,550 --> 00:12:38,310
+عن محطة بتوفر ظروف لا هوائية فيها أن ممكن أنا أدخل
+
+182
+00:12:38,310 --> 00:12:42,640
+إيديا من خلال هاي ممكن أنا أقدر أشتغلوهي بتوفر
+
+183
+00:12:42,640 --> 00:12:46,600
+لظروف لاهوائية Safety Cabinet بتوفر الأمان و
+
+184
+00:12:46,600 --> 00:12:52,640
+الحماية ومن خلالها أنا بكون شغلي كله بمنع ال
+
+185
+00:12:52,640 --> 00:12:57,580
+contamination بتتعقم من خلال ال UV في UV طبعا بكون
+
+186
+00:12:57,580 --> 00:13:01,100
+موجود في هذا Safety Cabinet بتعقم كل المكان اللي
+
+187
+00:13:01,100 --> 00:13:03,840
+أنا بشتغل فيه وهيك بضمن أن والله مافيه عندي أي
+
+188
+00:13:03,840 --> 00:13:06,780
+contamination هاي بالنسبة للأدوات اللي احنا
+
+189
+00:13:06,780 --> 00:13:10,630
+بنستعملها في معمن الـ Microbiologyطبعاً في أدوات
+
+190
+00:13:10,630 --> 00:13:13,310
+هنتعرف عليها في التفصيل كيف قاليه يتعملها في
+
+191
+00:13:13,310 --> 00:13:17,650
+المقشبات الأخرى و هنتعرف على الأدوات ان شاء الله
+
+192
+00:13:17,650 --> 00:13:21,950
+لما نشتغل العملين ان شاء الله تكون الأمور واضحة
+
+193
+00:13:21,950 --> 00:13:25,250
+انكوا هذا بالنسبة لأول chapter في معمل ال
+
+194
+00:13:25,250 --> 00:13:28,530
+microbiology والسلام عليكم ورحمة الله وبركاته
+
diff --git a/PL9fwy3NUQKwZWgAb_V1ATlRRsH-6i3cPC/o7otBG9cVEk.srt b/PL9fwy3NUQKwZWgAb_V1ATlRRsH-6i3cPC/o7otBG9cVEk.srt
new file mode 100644
index 0000000000000000000000000000000000000000..de3b927bfcc8e987362cc3790d22b5e0b7d248b7
--- /dev/null
+++ b/PL9fwy3NUQKwZWgAb_V1ATlRRsH-6i3cPC/o7otBG9cVEk.srt
@@ -0,0 +1,7 @@
+1
+00:00:20,640 --> 00:00:24,440
+مساكم معكم بسلسلة ميكروبيولوجيا الجامعة
+
+2
+00:00:24,440 --> 00:00:28,280
+الإسلامية، فيديو اليوم بعنوان Simplistan
diff --git a/PL9fwy3NUQKwZWgAb_V1ATlRRsH-6i3cPC/o7otBG9cVEk_postprocess.srt b/PL9fwy3NUQKwZWgAb_V1ATlRRsH-6i3cPC/o7otBG9cVEk_postprocess.srt
new file mode 100644
index 0000000000000000000000000000000000000000..9b9667090a313e1dc286b5a04dbabb26c48177ea
--- /dev/null
+++ b/PL9fwy3NUQKwZWgAb_V1ATlRRsH-6i3cPC/o7otBG9cVEk_postprocess.srt
@@ -0,0 +1,8 @@
+1
+00:00:20,640 --> 00:00:24,440
+مساكمل معاكم سلسلة ميكروبيولوجيا الجامعة
+
+2
+00:00:24,440 --> 00:00:28,280
+الإسلامية، فيديو اليوم بعنوان Simplistan
+
diff --git a/PL9fwy3NUQKwZWgAb_V1ATlRRsH-6i3cPC/o7otBG9cVEk_raw.json b/PL9fwy3NUQKwZWgAb_V1ATlRRsH-6i3cPC/o7otBG9cVEk_raw.json
new file mode 100644
index 0000000000000000000000000000000000000000..9f33480c0709883a5e71f46361da7f4144481c0e
--- /dev/null
+++ b/PL9fwy3NUQKwZWgAb_V1ATlRRsH-6i3cPC/o7otBG9cVEk_raw.json
@@ -0,0 +1 @@
+{"segments": [{"id": 1, "seek": 2828, "start": 20.64, "end": 28.28, "text": "مساكمل معاكم سلسلة ميكروبيولوجيا الجامعة الإسلامية، فيديو اليوم بعنوان Simplistan", "tokens": [2304, 3794, 995, 24793, 1211, 20449, 995, 24793, 8608, 1211, 3794, 37977, 3714, 1829, 4117, 32887, 21292, 12610, 29245, 25528, 25724, 10943, 27884, 33688, 3794, 37440, 10632, 12399, 8978, 16254, 2407, 45595, 20498, 45030, 1863, 2407, 7649, 3998, 564, 7559], "avg_logprob": -0.30659297617470344, "compression_ratio": 1.2767857142857142, "no_speech_prob": 1.0311603546142578e-05, "words": [{"start": 20.64, "end": 22.04, "word": "مساكمل", "probability": 0.613720703125}, {"start": 22.04, "end": 22.52, "word": " معاكم", "probability": 0.8338216145833334}, {"start": 22.52, "end": 23.04, "word": " سلسلة", "probability": 0.9786376953125}, {"start": 23.04, "end": 23.96, "word": " ميكروبيولوجيا", "probability": 0.85784912109375}, {"start": 23.96, "end": 24.44, "word": " الجامعة", "probability": 0.9754231770833334}, {"start": 24.44, "end": 25.66, "word": " الإسلامية،", "probability": 0.838330078125}, {"start": 25.66, "end": 25.98, "word": " فيديو", "probability": 0.9527994791666666}, {"start": 25.98, "end": 26.4, "word": " اليوم", "probability": 0.97021484375}, {"start": 26.4, "end": 27.34, "word": " بعنوان", "probability": 0.8939208984375}, {"start": 27.34, "end": 28.28, "word": " Simplistan", "probability": 0.3711140950520833}], "temperature": 1.0}], "language": "ar", "language_probability": 1.0, "duration": 127.825875, "duration_after_vad": 9.076250000000005}
\ No newline at end of file
diff --git a/PL9fwy3NUQKwZWgAb_V1ATlRRsH-6i3cPC/o7otBG9cVEk_raw.srt b/PL9fwy3NUQKwZWgAb_V1ATlRRsH-6i3cPC/o7otBG9cVEk_raw.srt
new file mode 100644
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--- /dev/null
+++ b/PL9fwy3NUQKwZWgAb_V1ATlRRsH-6i3cPC/o7otBG9cVEk_raw.srt
@@ -0,0 +1,8 @@
+1
+00:00:20,640 --> 00:00:24,440
+مساكمل معاكم سلسلة ميكروبيولوجيا الجامعة
+
+2
+00:00:24,440 --> 00:00:28,280
+الإسلامية، فيديو اليوم بعنوان Simplistan
+
diff --git a/PL9fwy3NUQKwZWgAb_V1ATlRRsH-6i3cPC/owBu12fuBog.srt b/PL9fwy3NUQKwZWgAb_V1ATlRRsH-6i3cPC/owBu12fuBog.srt
new file mode 100644
index 0000000000000000000000000000000000000000..d7e2cdfd2568cf56f55bef668bfcf410c1a478a3
--- /dev/null
+++ b/PL9fwy3NUQKwZWgAb_V1ATlRRsH-6i3cPC/owBu12fuBog.srt
@@ -0,0 +1,2758 @@
+1
+00:00:03,170 --> 00:00:07,030
+السلام عليكم ورحمة الله وبركاته، يعطيكم العافية
+
+2
+00:00:07,030 --> 00:00:11,070
+جميعًا، نستكمل معكم سلسلة محاضرات مساق الـ
+
+3
+00:00:11,070 --> 00:00:15,390
+Microbiology العملي. في هذا اللقاء، ستكون المحاضرة
+
+4
+00:00:15,390 --> 00:00:19,810
+بعنوان Single Media Multiple Test في المعامل.
+
+5
+00:00:19,810 --> 00:00:24,990
+السابقة، كنا نستخدم ميديا تكشف عن فحص كيميائي
+
+6
+00:00:24,990 --> 00:00:29,680
+واحد (one biochemical test). اليوم سنتعامل مع ميديا
+
+7
+00:00:29,680 --> 00:00:34,240
+تكشف عن أكثر من biochemical test، بقدر أُعمل فيها
+
+8
+00:00:34,240 --> 00:00:39,060
+أكثر من فحص كيميائي. من الأمثلة على high-end media
+
+9
+00:00:39,060 --> 00:00:43,980
+في عندي أكثر من ميديا، بقدر من خلالها أكشف عن أكثر
+
+10
+00:00:43,980 --> 00:00:49,440
+من biochemical tests: SIM media, Triple Sugar Iron agar,
+
+11
+00:00:49,860 --> 00:00:54,300
+Lysine Iron agar, Motility Indole, Ornithine Media. يبقى هذه الميديا من الأمثلة
+
+12
+00:00:54,300 --> 00:00:59,360
+على Single Media Multiple Test. اسم الميديا يأتي من
+
+13
+00:00:59,360 --> 00:01:04,720
+الفحوصات التي أقدر أُعملها في هذه الميديا.
+
+14
+00:01:04,720 --> 00:01:08,360
+سنتعرف في هذا المعمل على كل ميديا من هذه الميديات
+
+15
+00:01:08,360 --> 00:01:13,160
+بالتفصيل،  ما هي الفحوصات التي تكشف عنها كل ميديا، وكيف
+
+16
+00:01:13,160 --> 00:01:17,630
+أقدر أفسر نتائج هذه الميديا.  لنبدأ بأول
+
+17
+00:01:17,630 --> 00:01:23,930
+ميديا، وهي SIM media. قلنا أن اسم الميديا
+
+18
+00:01:23,930 --> 00:01:28,690
+يأتي من أسماء الفحوصات التي أقدر أُعملها في هذه
+
+19
+00:01:28,690 --> 00:01:32,850
+الميديا. بالنسبة لـ SIM media، الـ S يرمز لـ
+
+20
+00:01:32,850 --> 00:01:37,690
+sulfide، الـ I يرمز لـ Indole، والـ M يرمز لـ Motility.
+
+21
+00:01:37,690 --> 00:01:44,340
+يبقى أقدر أكشف عن الـ sulfide، عن الـ Indole، وعن
+
+22
+00:01:44,340 --> 00:01:48,480
+الـ motility.  تعرفنا في المعامل السابقة كيف أقدر
+
+23
+00:01:48,480 --> 00:01:52,260
+أكشف عن الـ motility وعن الـ Indole، لكن ما
+
+24
+00:01:52,260 --> 00:01:56,320
+تعرفنا على الـ sulfide. اليوم إن شاء الله سنتكلم
+
+25
+00:01:56,320 --> 00:01:59,560
+كيف أقدر أكشف عن الـ H2S production test. عن أي
+
+26
+00:01:59,560 --> 00:02:05,000
+أنزيم بالضبط يكشف هذا الفحص؟ ما هي نتائج الفحص؟ وماذا
+
+27
+00:02:05,000 --> 00:02:09,820
+يعطينا؟ الـ Indole والـ motility تعرفنا عليهما، سنمر
+
+28
+00:02:09,820 --> 00:02:13,720
+عليهما بسرعة. بالنسبة لأول فحص، وهو الـ
+
+29
+00:02:17,520 --> 00:02:21,640
+sulfide،  الـ sulfide، أكشف عن الـ H2S production
+
+30
+00:02:21,640 --> 00:02:27,480
+test. أريد أن أكشف عن إنزيم Sodium Thiosulfate
+
+31
+00:02:27,480 --> 00:02:31,980
+Reductase. يبقى من اسمه Sodium Thiosulfate
+
+32
+00:02:31,980 --> 00:02:37,100
+Reductase، سيعمل reduction لـ Sodium Thiosulfate.
+
+33
+00:02:37,510 --> 00:02:40,350
+يبقى الـ substrate التي يجب أن تكون موجودة في الـ
+
+34
+00:02:40,350 --> 00:02:43,750
+media هي Sodium Thiosulfate. يبقى هذا الـ substrate
+
+35
+00:02:43,750 --> 00:02:48,010
+التي سيعمل عليها إنزيم Sodium Thiosulfate
+
+36
+00:02:48,010 --> 00:02:54,310
+Reductase، سيعمل لها reduction. ناتج عملية الاختزال
+
+37
+00:02:54,310 --> 00:02:59,590
+هو الـ sulfide. في النهاية، سيتكون hydrogen sulfide gas
+
+38
+00:02:59,590 --> 00:03:06,090
+الذي هو H2S gas. سيتكون عندي H2S gas. كيف أُقدر أكشف
+
+39
+00:03:06,090 --> 00:03:12,270
+عن الـ H2S gas؟  قبل كنا نقول في الـ
+
+40
+00:03:12,270 --> 00:03:17,110
+fermentation، كان ناتج العملية Gas. كنا نضيف، نحضر
+
+41
+00:03:17,110 --> 00:03:20,210
+Durham tube. إذا تكون air bubble، أعرف أنه
+
+42
+00:03:20,210 --> 00:03:24,010
+تكون عندي Gas. لكن في هذه الـ media، هذه الـ media
+
+43
+00:03:24,010 --> 00:03:30,270
+تُعتبر،  التي هي فيها نسبة agar، هي semi-solid. بما أن
+
+44
+00:03:30,270 --> 00:03:33,330
+في عندي فحص motility، ستكون semi-solid media.
+
+45
+00:03:34,210 --> 00:03:37,530
+الفحص الـ fermentation، كانت الـ media broth. فبالتالي
+
+46
+00:03:37,530 --> 00:03:41,730
+سهل أن أضع Durham tube. لكن في هذا الفحص، ما
+
+47
+00:03:41,730 --> 00:03:46,290
+أقدر أضع Durham tube، لأن الـ media متصلبة، فيها نسبة
+
+48
+00:03:46,290 --> 00:03:49,670
+agar. فبالتالي، صعب أني أضع Durham tube، ما أقدر
+
+49
+00:03:49,670 --> 00:03:54,670
+أكشف عن الجاز باستخدام Durham tube. كيف أُقدر أكشف
+
+50
+00:03:54,670 --> 00:04:00,530
+عن الـ gas؟ الـ H2S gas؟  أقول لكم: أكشف عن الـ H2S gas
+
+51
+00:04:00,530 --> 00:04:04,910
+بإضافة indicator. هذا الـ indicator سيرتبط بالـ H2S
+
+52
+00:04:04,910 --> 00:04:10,390
+gas، ويعطيني لون أسود. يبقى لو البكتيريا عندها إنزيم
+
+53
+00:04:10,740 --> 00:04:15,020
+Sodium Thiosulfate Reductase، ستعمل اختزالًا للـ
+
+54
+00:04:15,020 --> 00:04:17,740
+substrate، وهي Sodium Thiosulfate، الموجودة في
+
+55
+00:04:17,740 --> 00:04:23,260
+الميديا، سينتج H2S gas. كيف أُقدر أكشف عنه؟ الـ H2S gas
+
+56
+00:04:23,260 --> 00:04:26,840
+سيرتبط بـ indicator. هذا الـ indicator موجود في
+
+57
+00:04:26,840 --> 00:04:30,640
+الميديا، اسمه Ferrous Ammonium Sulfate. يبقى هذا هو
+
+58
+00:04:30,640 --> 00:04:35,080
+الـ H2S indicator. سيرتبط مع الـ H2S gas. لو
+
+59
+00:04:35,080 --> 00:04:38,460
+البكتيريا عندها إنزيم Sodium Thiosulfate Reductase،
+
+60
+00:04:38,460 --> 00:04:43,920
+وتكون الـ H2S gas، سيتكون ferrous sulfide. هذا المركب
+
+61
+00:04:43,920 --> 00:04:50,160
+black precipitate، لونه أسود، سيتكون هذا الراسب. يبقى
+
+62
+00:04:50,160 --> 00:04:54,060
+لو تكون عندي لون أسود، معناه أن البكتيريا عندها
+
+63
+00:04:54,060 --> 00:04:58,620
+الـ إنزيم، تكون غاز، تفاعل الغاز مع، تفاعل الغاز مع الـ
+
+64
+00:04:58,620 --> 00:05:01,520
+indicator، وهو ferrous ammonium sulfate، الـ H2S
+
+65
+00:05:01,520 --> 00:05:05,080
+indicator، الموجود في الـ media، وأعطاني اللون
+
+66
+00:05:05,080 --> 00:05:11,250
+الأسود. طيب، بالنسبة لفحص الـ H2S production، قلنا
+
+67
+00:05:11,250 --> 00:05:15,610
+أنه إذا تكون اللون الأسود، يبقى هذا positive،
+
+68
+00:05:15,610 --> 00:05:19,550
+تحتوي على إنزيم Sodium Thiosulfate Reductase. عرفنا
+
+69
+00:05:19,550 --> 00:05:24,590
+الـ substrates، عرفنا الـ enzyme، عرفنا الـ indicator
+
+70
+00:05:24,590 --> 00:05:29,820
+الموجود في الـ medium. طيب، كأي فحص كيميائي، أكيد
+
+71
+00:05:29,820 --> 00:05:34,200
+البكتيريا تعمل، أو عندها هذا الإنزيم، يكون لهدف،
+
+72
+00:05:34,200 --> 00:05:38,460
+يكون فيه فائدة من وجود هذا الإنزيم في الـ in vivo.
+
+73
+00:05:38,460 --> 00:05:41,700
+أنا أتكلم عن in vivo. زي ما قلنا مثلًا في فحص
+
+74
+00:05:41,700 --> 00:05:45,960
+الكتلاز، كانت تتخلص من الـ hydrogen peroxide، الـ
+
+75
+00:05:45,960 --> 00:05:50,480
+toxic. هنا، الفائدة من أن البكتيريا تعمل reduction
+
+76
+00:05:50,480 --> 00:05:55,780
+للسلفايت، لأنه قلنا من ضمن الـ inorganic molecules
+
+77
+00:05:55,780 --> 00:05:59,980
+التي تستعملها البكتيريا كمستقبل نهائي للإلكترونات
+
+78
+00:05:59,980 --> 00:06:04,120
+في الـ anaerobic respiration، عشان تنتج الطاقة.
+
+79
+00:06:04,120 --> 00:06:09,280
+الـ sulfur. يبقى هذا من فوائد هذا الإنزيم، أنه تعمل
+
+80
+00:06:09,280 --> 00:06:13,310
+reduction للسلفايت، عشان أستخدم الـ Sulphur
+
+81
+00:06:13,310 --> 00:06:16,150
+كمستقبل نهائي للإلكترونات في الـ anaerobic
+
+82
+00:06:16,150 --> 00:06:20,190
+respiration، لإنتاج الطاقة. طيب، خلّصنا من أول فحص
+
+83
+00:06:20,190 --> 00:06:25,830
+وهو الـ H2S Production Test. نجي للفحص الثاني،
+
+84
+00:06:25,830 --> 00:06:30,530
+الـ Indole. طبعًا الـ Indole مر معنا في الـ chapter الذي
+
+85
+00:06:30,530 --> 00:06:35,050
+قبله. قلنا الـ Indole يكشف عن إنزيم tryptophanase.
+
+86
+00:06:35,050 --> 00:06:38,970
+tryptophanase، من اسمه، سيعمل على الـ substrate
+
+87
+00:06:38,970 --> 00:06:42,770
+tryptophan، وهي الـ amino acid. هذه الـ tryptophan
+
+88
+00:06:42,770 --> 00:06:47,450
+تتكون من Indole، و pyruvate، و NH3، مرتبطة ببعض. لو
+
+89
+00:06:47,450 --> 00:06:51,770
+إنزيم tryptophanase موجود في البكتيريا، سيكسر
+
+90
+00:06:51,770 --> 00:06:58,590
+الـ tryptophan إلى Indole، pyruvate، و NH3. سنضيف الـ Kovacs
+
+91
+00:06:58,590 --> 00:07:01,950
+reagent.  نضيف الـ Kovacs reagent. هذا الـ reagent
+
+92
+00:07:01,950 --> 00:07:07,350
+نضيفه بعد الـ incubation. نضيفه على الـ media. هذا
+
+93
+00:07:07,350 --> 00:07:11,710
+الـ Kovacs reagent سيرتبط مع الـ Indole، ناتج من نواتج
+
+94
+00:07:11,710 --> 00:07:16,690
+عملية تكسير الـ tryptophan. سيرتبط به، ويتكون red ring.
+
+95
+00:07:16,690 --> 00:07:20,010
+قلنا هذا الـ red ring، يكون لونه لون الكرز، الـ
+
+96
+00:07:20,010 --> 00:07:23,950
+cherry red ring. لو تكونت، يبقى هي Indole positive.
+
+97
+00:07:23,950 --> 00:07:28,010
+بالنسبة لفحص الـ motility، اتكلمنا،  أنا، لأُعمل
+
+98
+00:07:28,010 --> 00:07:32,190
+فحص الـ motility، أزرع البكتيريا في ميديا semi-solid
+
+99
+00:07:32,190 --> 00:07:37,170
+media. هي الميديا فيها نسبة agar 4 من 10% agar.
+
+100
+00:07:37,170 --> 00:07:43,190
+الفكرة أنه لو البكتيريا متحركة، سألاحظ أن
+
+101
+00:07:43,190 --> 00:07:48,550
+النمو سيكون على خط الزراعة، وسينتشر بعيدًا عن خط
+
+102
+00:07:48,550 --> 00:07:52,350
+الزراعة. لكن لو كانت non-motile، فقط على خط الزراعة،
+
+103
+00:07:52,350 --> 00:07:56,790
+سأجد النمو. هذا بالنسبة لفحص الـ motility. طبعًا أنا
+
+104
+00:07:56,790 --> 00:08:01,450
+سأعمل stabbing. يبقى ميديا الـ SIM، أي ميديا فيها فحص
+
+105
+00:08:01,450 --> 00:08:05,650
+الـ motility، ستكون الزراعة stabbing، ستكون stab tube.
+
+106
+00:08:05,650 --> 00:08:08,770
+سأزرع stabbing. سآخذ البكتيريا التي أريد أن أكشف عنها،
+
+107
+00:08:08,770 --> 00:08:13,830
+سأعمل stabbing للـ media، وسأعمل incubation لمدة 24
+
+108
+00:08:14,320 --> 00:08:20,760
+ساعة.  هناك ملاحظتان في هذه الـ media.
+
+109
+00:08:20,760 --> 00:08:25,480
+الملاحظة الأولى، أني بعد 24 ساعة، أقرأ
+
+110
+00:08:25,480 --> 00:08:31,880
+النتائج،  وهي فحوصات هذه الـ media. أقول لكم: أقرأ فحص الـ
+
+111
+00:08:31,880 --> 00:08:37,300
+motility، بعدين أقرأ فحص الـ sulfide، بعدين أقرأ فحص
+
+112
+00:08:37,300 --> 00:08:42,610
+الـ Indole، من خلال إضافة الـ Kovacs reagent. يبقى لازم،
+
+113
+00:08:42,610 --> 00:08:48,450
+أو ممنوع، أقرأ فحص الـ Indole إلا بعد قراءة فحص الـ
+
+114
+00:08:48,450 --> 00:08:52,170
+motility. لماذا؟ لأن مكتوب بالترتيب، أقرأ فحص الـ
+
+115
+00:08:52,170 --> 00:08:56,030
+motility، بعدين الـ H2S production، بعدين أضيف الـ
+
+116
+00:08:56,030 --> 00:09:00,070
+Kovacs reagent، عشان أقرأ الـ Indole test. هكذا بالترتيب.
+
+117
+00:09:00,070 --> 00:09:03,910
+ممنوع أضيف الـ Kovacs reagent، وأقرأ الـ Indole test،
+
+118
+00:09:03,910 --> 00:09:09,450
+بعدين أقرأ فحص الـ motility. هذا سؤال مهم. السبب أن
+
+119
+00:09:09,940 --> 00:09:15,040
+الـ Kovacs reagent، قلنا أحد مكوناته الـ HCL، ذكرنا
+
+120
+00:09:15,040 --> 00:09:19,260
+سابقًا، لما شرحنا فحص الـ Indole test بالتفصيل، أحد
+
+121
+00:09:19,260 --> 00:09:24,440
+مكونات الـ Kovacs reagent هو الـ HCL. أقول لكم: الـ HCL
+
+122
+00:09:24,440 --> 00:09:28,900
+يقلل الـ turbidity الموجودة في الميديا، ويجعلها
+
+123
+00:09:28,900 --> 00:09:34,020
+clear، يعمل clearance للميديا. طيب، فحص الـ motility
+
+124
+00:09:34,020 --> 00:09:39,000
+يعتمد على الـ turbidity، أنه لو البكتيريا كانت على
+
+125
+00:09:39,000 --> 00:09:42,060
+خط الزراعة، نمت على خط الزراعة، واتجهت بعيدًا عن خط
+
+126
+00:09:42,060 --> 00:09:44,700
+الزراعة، يكون فيها turbidity عالية. أقرأ الـ
+
+127
+00:09:44,700 --> 00:09:47,600
+turbidity، فأعرف أنها motile. لكن لو كانت فقط على
+
+128
+00:09:47,600 --> 00:09:52,220
+خط الزراعة، يبقى non-motile. يبقى قراءة فحص الـ
+
+129
+00:09:52,220 --> 00:09:56,020
+motility تعتمد على الـ turbidity. طب الـ Kovacs
+
+130
+00:09:56,020 --> 00:09:59,280
+reagent، قلنا أنه يقلل الـ turbidity، يعمل
+
+131
+00:09:59,280 --> 00:10:03,790
+clearance للميديا. فبالتالي، لن أقدر أن أقرأ فحص الـ
+
+132
+00:10:03,790 --> 00:10:09,410
+motility، صح؟ لأنه يعتمد على الـ turbidity.  لذلك،
+
+133
+00:10:09,410 --> 00:10:13,690
+أقرأ فحص الـ motility، أشوف هل هي على خط الزراعة
+
+134
+00:10:13,690 --> 00:10:18,490
+فقط، يبقى non-motile. هل هي خرجت عن خط الزراعة، اتجهت
+
+135
+00:10:18,490 --> 00:10:23,350
+بعيدًا عن خط الزراعة؟ يبقى هي motile. وبعدين أسجل
+
+136
+00:10:23,350 --> 00:10:27,210
+النتيجة، وبعدين أضيف الـ Kovacs reagent، عشان أقرأ الـ
+
+137
+00:10:27,210 --> 00:10:32,950
+Indole test. هذا بالنسبة لأول ملاحظة. الملاحظة الثانية،
+
+138
+00:10:32,950 --> 00:10:38,250
+أنه لو البكتيريا H2S positive، لو كان عندها إنزيم
+
+139
+00:10:38,250 --> 00:10:43,770
+Sodium Thiosulfate Reductase، وأنتجت الـ H2S gas، ارتبط
+
+140
+00:10:43,770 --> 00:10:47,090
+مع الـ H2S indicator، وأعطاني ferrous sulfide، الذي
+
+141
+00:10:47,090 --> 00:10:51,950
+يُعتبر black precipitate، تكون عندي لون أسود غامق
+
+142
+00:10:51,950 --> 00:10:57,360
+في كل الـ media. طب، هل سأعرف نتيجة الـ motility لو
+
+143
+00:10:57,360 --> 00:11:00,940
+تكون لون أسود في الـ media؟ أكيد لا، لن أعرف نتيجة
+
+144
+00:11:00,940 --> 00:11:05,220
+الـ motility. بالتالي، لو البكتيريا كانت H2S
+
+145
+00:11:05,220 --> 00:11:10,020
+production positive، الـ motility لن أقدر أن أعرفها،
+
+146
+00:11:10,020 --> 00:11:15,520
+أكتب في النتيجة unknown. يبقى لو جاءني سؤال، صورة
+
+147
+00:11:15,520 --> 00:11:20,460
+موجودة عليها H2S، كان لونها أسود، H2S positive، فحص
+
+148
+00:11:20,460 --> 00:11:23,800
+الـ motility، نكتب unknown، لن أقدر أن أعرف فحص
+
+149
+00:11:23,800 --> 00:11:28,180
+الـ motility، لأن اللون الأسود غطّى على الـ media، غطّى
+
+150
+00:11:28,180 --> 00:11:31,920
+على فحص الـ motility، وعلى الـ turbidity. طيب، ما هو الحل
+
+151
+00:11:31,920 --> 00:11:35,940
+لعرف هل البكتيريا motile أم non-motile؟ نقول لكم:
+
+152
+00:11:35,940 --> 00:11:40,760
+نحضر media خاصة، semi-solid media، فيها نسبة agar
+
+153
+00:11:40,760 --> 00:11:46,690
+4 من 10%، بدون أن أضيف Sodium
+
+154
+00:11:46,690 --> 00:11:50,490
+Thiosulfate، الـ substrate، والـ H2S indicator، بدون
+
+155
+00:11:50,490 --> 00:11:54,990
+أن أضيف أي substrate أو أي indicator لأي فحوصات
+
+156
+00:11:54,990 --> 00:11:59,410
+أخرى،  زي ما عملنا في أول فحص في الـ motility،
+
+157
+00:11:59,410 --> 00:12:03,510
+كانت هي فقط high-end medium، nutrient broth، مع نسبة
+
+158
+00:12:03,510 --> 00:12:07,990
+agar 4 من 10%.  ليس فيها أي substrate لأي فحوصات
+
+159
+00:12:07,990 --> 00:12:11,810
+كيميائية أخرى، فهي فقط ستكشف عن الـ motility. هذا
+
+160
+00:12:11,810 --> 00:12:16,250
+بالنسبة للحلقة. كما نكون قد  أنهينا تقريبًا أو  أجملنا الـ
+
+161
+00:12:16,250 --> 00:12:20,430
+SIM media، عن ماذا الفحوصات التي تكشف عنها، وما
+
+162
+00:12:20,430 --> 00:12:24,150
+هي النتيجة positive و negative لكل فحص، كيف أُقدر
+
+163
+00:12:24,150 --> 00:12:29,410
+أُعمل هذا الفحص، وكيف أُقدر أقرأ النتائج. نجي للـ
+
+164
+00:12:29,410 --> 00:12:34,690
+slides. هذه الـ media، قلنا semi-solid media، SIM test.
+
+165
+00:12:34,690 --> 00:12:37,910
+medium is a semi-solid media، يعني نسبة agar فيها
+
+166
+00:12:37,910 --> 00:12:42,590
+4 من 10%. قلنا أي ميديا فيها فحص الـ motility، فهي
+
+167
+00:12:42,590 --> 00:12:47,120
+semi-solid media. Used as a differential test medium.
+
+168
+00:12:47,120 --> 00:12:51,360
+أكيد، بما أنها تعطيني، أو تُفرّق لي بين بكتيريا وبكتيريا،
+
+169
+00:12:51,360 --> 00:12:55,900
+يبقى هي differential test media.  فأعطت مثلًا بعض
+
+170
+00:12:55,900 --> 00
+
+223
+00:16:29,150 --> 00:16:32,030
+nature of the media نتيجة أن الـ media هي semi
+
+224
+00:16:32,030 --> 00:16:35,310
+-solid فبالتالي لو البكتيريا عندها القدرة أن
+
+225
+00:16:35,310 --> 00:16:39,310
+تنمو radiating out from the center step line لو
+
+226
+00:16:39,310 --> 00:16:43,190
+قدرت تنمو بعيدا عن خط الزراعة يبقى هذا يشير أن
+
+227
+00:16:43,190 --> 00:16:48,320
+البكتيريا أو الـ test organism is motile بالنسبة لـ
+
+228
+00:16:48,320 --> 00:16:53,340
+Significant لهذا الفحص قلنا أي فحص بيكون له
+
+229
+00:16:53,340 --> 00:16:56,500
+أهمية Significant عشان يفرق بين من
+
+230
+00:16:56,500 --> 00:17:00,000
+ومن فحص الـ Motility تكلمنا عنه لما شرحنا على
+
+231
+00:17:00,000 --> 00:17:02,820
+البكتيريا الـ Motility والـ Indoor لما شرحناهم
+
+232
+00:17:02,820 --> 00:17:07,880
+بالتفصيل لكن بالنسبة للـ H2S production، الـ H2S
+
+233
+00:17:07,880 --> 00:17:12,060
+production بقول لي أن الـ significant لهذا الفحص
+
+234
+00:17:12,060 --> 00:17:15,940
+the ability to reduce sulfur is specific to two
+
+235
+00:17:15,940 --> 00:17:19,140
+members of the enteric family Salmonella and
+
+236
+00:17:19,140 --> 00:17:24,680
+Brucella بقول لي أن قدرة البكتيريا أنها تعمل اختصار
+
+237
+00:17:24,680 --> 00:17:30,660
+للسلفر فقط بكتيريتين هما اللتان  H2S production
+
+238
+00:17:30,660 --> 00:17:34,140
+positive من الـ Enteric family اللي هي عائلة الـ
+
+239
+00:17:34,140 --> 00:17:38,520
+Enterobacteriaceae السلمونيلا والبروتياس هما
+
+240
+00:17:38,520 --> 00:17:43,940
+هذان H2S positive، احنا قلنا إذا بتذكروا بفحص
+
+241
+00:17:43,940 --> 00:17:49,520
+البروتياس، بفحص اليورياز، sorry، بفحص اليورياز قلنا
+
+242
+00:17:49,520 --> 00:17:54,400
+يفرق بين الـ non-lactose fermenter اللي هم
+
+243
+00:17:54,400 --> 00:17:58,600
+السلمونيلا، شجيلة، البروتياس، هؤلاء الثلاثة يعتبرون
+
+244
+00:17:58,600 --> 00:18:02,930
+non-lactose fermenter موجودين في عينات الـ stone
+
+245
+00:18:02,930 --> 00:18:07,370
+قلنا عشان افرق بينهم بعمل فحص اليورياز، لو طلعت
+
+246
+00:18:07,370 --> 00:18:11,670
+يورياز positive يبقى بروتياس، لو طلعت يورياز
+
+247
+00:18:11,670 --> 00:18:16,690
+negative يبقى سلمونيلا أو شجيلة، طيب السلمونيلا و
+
+248
+00:18:16,690 --> 00:18:21,590
+الشجيلة، كيف بدي افرق بينهم؟ بقول من خلال فحص الـ
+
+249
+00:18:21,590 --> 00:18:27,450
+motility والـ H2S production، السلمونيلا H2S
+
+250
+00:18:27,450 --> 00:18:32,390
+production positive وmotile، لكن شجيلة non motile و
+
+251
+00:18:32,390 --> 00:18:38,470
+non H2S أو تعتبر H2S production negative، البروتياس
+
+252
+00:18:38,470 --> 00:18:43,310
+نفس السلمونيلا motile و H2S positive، لكن كيف
+
+253
+00:18:43,310 --> 00:18:46,830
+بفرق بينهم؟ من خلال فحص اليورياز، يبقى هي مكتوب
+
+254
+00:18:46,830 --> 00:18:51,650
+السلمونيلا H2S positive and motile، للبروتياس H2S
+
+255
+00:18:51,650 --> 00:18:54,810
+positive and motile، كيف بفرق بينهم؟ من خلال، اتكلمنا
+
+256
+00:18:54,810 --> 00:18:57,950
+عن هذا الشيء سابقا، من خلال فحص اليورياز،
+
+257
+00:18:57,950 --> 00:19:03,370
+السلمونيلا يورياز negative، للبروتياس positive،
+
+258
+00:19:03,370 --> 00:19:10,470
+الشجيلة عكس السلمونيلا، والبروتياس H2S negative and
+
+259
+00:19:10,470 --> 00:19:15,280
+non motile، لكن هي يورياز negative، الـ E. coli
+
+260
+00:19:15,280 --> 00:19:20,480
+اتفقنا أنه هي فقط الوحيدة الـ non-motile من
+
+261
+00:19:20,480 --> 00:19:25,900
+الكوليفارم and doesn't produce H2S، الـ E. coli
+
+262
+00:19:25,900 --> 00:19:31,320
+تعتبر motile وكمان doesn't produce H2S، قلنا في
+
+263
+00:19:31,320 --> 00:19:35,080
+عندي فحص اللي هو الـ MVEC، بقدر أفرق بين الـ E. coli والـ
+
+264
+00:19:35,080 --> 00:19:39,360
+E. cloacae، الـ MVEC اللي هي بفرق بين الكوليفارم
+
+265
+00:19:39,360 --> 00:19:45,530
+حتى ككل، مش بس الـ E. cloacae والـ E. coli، كيف أنا بدي
+
+266
+00:19:45,530 --> 00:19:49,050
+أشغل هذا الفحص؟ أكيد بعمل label للي تيوب الـ SIM
+
+267
+00:19:49,050 --> 00:19:53,810
+أجار دي بتيوب، كيف بتكون؟ هي أجار من أشكال من أشكال
+
+268
+00:19:53,810 --> 00:19:58,510
+اللي هو الـ media، في الـ SIM بتكون أجار دي بتيوب
+
+269
+00:19:58,510 --> 00:20:03,270
+بعمل label باسم البكتيريا اللي بدي أكشف عنها و
+
+270
+00:20:03,270 --> 00:20:09,790
+بكتب طبعا اللي هو تاريخ الزراعة، بعمل طبعا الـ
+
+271
+00:20:09,790 --> 00:20:12,770
+stabbing، بستخدم الـ aseptic technique، بمنع أي كون
+
+272
+00:20:12,770 --> 00:20:17,950
+تاميناشي، أن سير، بعمل الـ stabbing للـ media، بحاول
+
+273
+00:20:17,950 --> 00:20:21,310
+أن أنا أبعد عن الـ bottom of the tube، آخر الـ tube
+
+274
+00:20:21,310 --> 00:20:27,610
+ما بعمل الـ stabbing له، تقريبا ببعد شوية مسافة، بعمل
+
+275
+00:20:27,610 --> 00:20:32,950
+incubation لمدة 24 ساعة، درجة حرارة 37، هنكتب
+
+276
+00:20:32,950 --> 00:20:36,230
+للملاحظة، قولناها سابقا، أن لو البكتيريا عندها القدرة
+
+277
+00:20:36,230 --> 00:20:40,190
+أنها تنتج الـ hydrogen sulfide يبقى بالنهاية هيتكون
+
+278
+00:20:40,190 --> 00:20:43,990
+black precipitate، فبالتالي مش هقدر اكشف على الـ
+
+279
+00:20:43,990 --> 00:20:48,410
+motility of organism، عشان هيك بفكر أن فحص الـ
+
+280
+00:20:48,410 --> 00:20:53,150
+motility هو false negative result، مش هيبين عندي، مش
+
+281
+00:20:53,150 --> 00:20:56,970
+هتبين الحركة، فبالتالي هفكرها negative، لكن هي false
+
+282
+00:20:56,970 --> 00:21:02,190
+negative result، بالـ H2S Production، اللون الأسود
+
+283
+00:21:02,190 --> 00:21:05,850
+اللي اتكون هو اللي خلاني ما أشوف الـ turbidity
+
+284
+00:21:05,850 --> 00:21:10,870
+فبالتالي أحدد الـ Motility للبكتيريا، عشان هيك الحل
+
+285
+00:21:10,870 --> 00:21:15,970
+أني أنا بعمل Semi Solid Media بدون ما يكون فيه
+
+286
+00:21:15,970 --> 00:21:19,010
+أي substrate اللي هي الـ Sodium Thiosulfate أو
+
+287
+00:21:19,010 --> 00:21:22,810
+الـ H2S Indicator، فقط بتتكون من الـ Nutrient
+
+288
+00:21:22,810 --> 00:21:33,130
+Growth مع نسبة أجار 0.4 من 10%، بالنسبة للنتائج، هي
+
+289
+00:21:33,130 --> 00:21:37,940
+الصورتان نفس الشيء، أول شيء هنقرأ فحص الـ Motility
+
+290
+00:21:37,940 --> 00:21:42,120
+أكيد، اللي بالنسبة لـ Motility هي الـ bacteria motile
+
+291
+00:21:42,120 --> 00:21:46,080
+اللي بنلاحظ أنه في عندي بعيدا عن خط الزراعة في
+
+292
+00:21:46,080 --> 00:21:50,860
+عندي turbidity، بعدها هقرأ الـ edge to edge، هي
+
+293
+00:21:50,860 --> 00:21:55,120
+negative، edge to edge negative، فحص الـ Indole، ضفنا
+
+294
+00:21:55,120 --> 00:21:58,980
+الـ Kovac's reagent، ما تحول اللون للون أحمر في أعلى
+
+295
+00:21:58,980 --> 00:22:03,080
+الحلقة، يبقى هي في أعلى الـ tube يبقى هي Indole
+
+296
+00:22:03,080 --> 00:22:08,130
+negative، هان بالنسبة لفحص الـ Motility، من اللحظة
+
+297
+00:22:08,130 --> 00:22:13,070
+أنه على خط الزراعة يبقى هي non-motile، فحص الـ H2S
+
+298
+00:22:13,070 --> 00:22:17,150
+negative، فحص الاندول positive، اتكون لون cherry red
+
+299
+00:22:17,150 --> 00:22:24,250
+يبقى هي positive، هان H2S positive، ماقدر اكشف على
+
+300
+00:22:24,250 --> 00:22:28,030
+الـ motility، فبكتب unknown، الـ motility بكتب عنها
+
+301
+00:22:28,030 --> 00:22:31,710
+unknown، بالنسبة للاندول negative،
+
+302
+00:22:34,450 --> 00:22:39,650
+هذه الـ tube هي كـ control، مش مزروعة، هذه الـ tube
+
+303
+00:22:39,650 --> 00:22:43,890
+نقرأ أول شيء نتيجة الـ motility، فقط هي هنا على خط
+
+304
+00:22:43,890 --> 00:22:49,330
+الزراعة هي، يبقى هي non motile، بالنسبة للـ H2S
+
+305
+00:22:49,330 --> 00:22:52,950
+negative، بالنسبة للـ Indole، من اللاحظ في عندي لون
+
+306
+00:22:52,950 --> 00:22:58,530
+أحمر يبقى هي Indole positive، هنا الـ H2S positive،
+
+307
+00:22:58,530 --> 00:23:02,780
+طبعا الـ motility unknown، بالنسبة للاندول positive،
+
+308
+00:23:02,780 --> 00:23:06,880
+هان الـ H2S positive، بالنسبة للموتيليتي unknown،
+
+309
+00:23:06,880 --> 00:23:10,500
+بالنسبة للاندول negative، يبقى هيك نقرأ النتيجة،
+
+310
+00:23:10,500 --> 00:23:16,380
+بنكتب الثلاث فحوصات SIM، عشان ما نطلع خبطة، نكتب S
+
+311
+00:23:16,380 --> 00:23:21,090
+اللي هو للكبريت، I للاندول، M للموتيليتي، نقرأ أول
+
+312
+00:23:21,090 --> 00:23:24,210
+شيء الـ motility، بعدين الـ H2S، بعدين بنضيف الـ
+
+313
+00:23:24,210 --> 00:23:27,910
+Kovac's reagent وبنقرأ نتيجة الاندول، هيك بنكون
+
+314
+00:23:27,910 --> 00:23:34,430
+خلصنا أول media اللي هي الـ SIM media، نيجي لتاني
+
+315
+00:23:34,430 --> 00:23:39,150
+media، تعتبر single media multiple test اللي هي لـ
+
+316
+00:23:39,150 --> 00:23:44,970
+triple sugar iron agar أو Kligler iron agar، هذان
+
+317
+00:23:44,970 --> 00:23:49,950
+الوسطين نفس الـ media، لكن الاختلاف أن الـ Kligler
+
+318
+00:23:49,950 --> 00:23:55,650
+iron agar بتحتوي على نوعين من أنواع السكر اللي هما
+
+319
+00:23:55,650 --> 00:24:00,410
+الـ dextrose اللي هو الجلوكوز زائد اللاكتوز، لكن الـ
+
+320
+00:24:00,410 --> 00:24:04,230
+triple sugar iron agar بتحتوي على ثلاثة أنواع من
+
+321
+00:24:04,230 --> 00:24:09,500
+السكريات، من اسمها triple، dextrose زي الكليجر، زائد
+
+322
+00:24:09,500 --> 00:24:13,080
+lactose برضه زي الكليجر، لكن الإضافة المختلفة عن
+
+323
+00:24:13,080 --> 00:24:17,160
+الكليجر اللي هي السكروز، يبقى الإثنان نفس الشيء
+
+324
+00:24:17,160 --> 00:24:22,880
+لكن الفرق أن سكر السكروز مش موجود في الكليجر
+
+325
+00:24:22,880 --> 00:24:27,580
+iron agar، فقط موجود في الـ triple sugar iron agar،
+
+326
+00:24:27,580 --> 00:24:33,690
+هي الـ media من خلالها بكشف على deamination للبيبتون،
+
+327
+00:24:33,690 --> 00:24:38,430
+الـ fermentation والـ H2S production، ففي هذه الـ
+
+328
+00:24:38,430 --> 00:24:44,810
+media، منصبها في slant، slant، هي بتكون slant tube،
+
+329
+00:24:44,810 --> 00:24:50,310
+ليه أنا صبيتها في slant tube؟ عشان أوفر ظروف
+
+330
+00:24:50,310 --> 00:24:54,810
+هوائية aerobic condition، من خلال الـ slant، سطح الـ
+
+331
+00:24:54,810 --> 00:24:58,680
+slant هذا موفر aerobic condition، وهذا الـ
+
+332
+00:24:58,680 --> 00:25:01,580
+deamination اللي للبيبتون بيحصل في ظروف aerobic
+
+333
+00:25:01,580 --> 00:25:07,060
+condition، كمان بدي أوفر من خلال الـ butt أو اللي تحت
+
+334
+00:25:07,060 --> 00:25:10,140
+الـ slant، هذا بسميه butt، اللي بكون موجود تحت، هذا
+
+335
+00:25:10,140 --> 00:25:13,920
+بسميه butt، هذا بوفر ظروف anaerobic condition، ظروف
+
+336
+00:25:13,920 --> 00:25:17,620
+لاهوائية، الـ fermentation والـ gas production
+
+337
+00:25:17,620 --> 00:25:23,030
+بيحصلوا في ظروف لاهوائية، عشان هيك بدي أنا أوفر
+
+338
+00:25:23,030 --> 00:25:27,710
+ظروف هوائية وظروف لاهوائية، فبصب الـ media بـ slant،
+
+339
+00:25:27,710 --> 00:25:30,730
+لأن الـ deamination للبيبتون بيصير في ظروف aerobic
+
+340
+00:25:30,730 --> 00:25:33,710
+condition، الـ fermentation والـ H2S production
+
+341
+00:25:33,710 --> 00:25:38,410
+بيصير في ظروف anaerobic condition، يبقى الـ slant
+
+342
+00:25:38,410 --> 00:25:41,930
+هكشف على الـ deamination للبيبتون، الـ butt هكشف على
+
+343
+00:25:41,930 --> 00:25:45,990
+الـ fermentation والـ H2S production، في high الـ
+
+344
+00:25:45,990 --> 00:25:50,800
+media، في عندي pH indicator، لأنه نواتج عملية الـ
+
+345
+00:25:50,800 --> 00:25:54,280
+fermentation acid، ونواتج عملية الـ deamination
+
+346
+00:25:54,280 --> 00:25:57,400
+للبيبتون، في عندي alkaline product، فبالتالي بما أنه
+
+347
+00:25:57,400 --> 00:26:00,680
+أتكون alkaline أو الـ acidic product، هضيف pH
+
+348
+00:26:00,680 --> 00:26:05,280
+Indicator عشان استدل على حدوث الـ fermentation أو
+
+349
+00:26:05,280 --> 00:26:09,940
+الـ deamination للبيبتون، الـ pH Indicator الموجود في
+
+350
+00:26:09,940 --> 00:26:13,600
+الـ media هو الـ phenol red، يعني الـ phenol red مر
+
+351
+00:26:13,600 --> 00:26:17,400
+معنا للمرة الثالثة، أول مرة مر في فحص الـ
+
+352
+00:26:17,400 --> 00:26:21,370
+fermentation، ثاني مرة في فحص اليورياز، ثالث مرة في
+
+353
+00:26:21,370 --> 00:26:25,530
+فحص triple sugar iron agar، حدوده في الـ acid
+
+354
+00:26:25,530 --> 00:26:28,990
+بيكون لونه yellow، في الـ alkaline بيكون لونه red، في
+
+355
+00:26:28,990 --> 00:26:32,990
+الـ neutral بيكون لونه orange، بما أني أنا بدي اكشف
+
+356
+00:26:32,990 --> 00:26:37,150
+على H2S production، يبقى اكيد لازم يكون موجود في الـ
+
+357
+00:26:37,150 --> 00:26:42,510
+media H2S indicator، زي فحص الـ SIM، لكن الـ H2S
+
+358
+00:26:42,510 --> 00:26:47,920
+indicator هو ferrous ammonium citrate، تمام، ferrous ammonium
+
+359
+00:26:47,920 --> 00:26:51,000
+citrate اللي موجود في هذه الـ media، الـ H2S
+
+360
+00:26:51,000 --> 00:26:56,100
+indicator، طيب لو البكتيريا عملت glucose
+
+361
+00:26:56,100 --> 00:26:59,120
+fermentation، يبقى هيتكون ناتج عملية الـ
+
+362
+00:26:59,120 --> 00:27:04,580
+fermentation acid، هيتحول لون الـ butt للون yellow، صح؟
+
+363
+00:27:04,580 --> 00:27:09,220
+لكن لو البكتيريا عملت deamination للبيبتون، هتنزع
+
+364
+00:27:09,220 --> 00:27:14,540
+الأمونيا، البيبتون عبارة عن، قلنا amino acid، الأمينو
+
+365
+00:27:14,540 --> 00:27:17,960
+أسيد، هيك هتبعث الأمونيا، عملت دي
+
+366
+00:27:17,960 --> 00:27:21,340
+amination،زالة هاي الأمونيا، يبقى الأمونيا عندي
+
+367
+00:27:21,340 --> 00:27:26,360
+alkaline product، هترفع الـ pH، هيتحول اللون، هيتحول
+
+368
+00:27:26,360 --> 00:27:30,170
+لون الـ media للون أحمر، لو البكتيريا عندها القدرة
+
+369
+00:27:30,170 --> 00:27:34,990
+أنها تعمل H2S production، قلنا هيتكون H2S gas،
+
+370
+00:27:34,990 --> 00:27:37,950
+هيرتبط مع الـ indicator الموجود في الـ media، هيتكون
+
+371
+00:27:37,950 --> 00:27:41,650
+عندنا black precipitate في الـ butt، يبقى هكذا أعرف أن
+
+372
+00:27:41,650 --> 00:27:48,750
+صار فيه عندنا H2S production، هذا بالنسبة لكيف أو  ما
+
+373
+00:27:48,750 --> 00:27:53,650
+اللي بيصير في هاي الـ media، هلأ لو صار فيه عندنا أو
+
+374
+00:27:53,650 --> 00:27:57,070
+أتكون لون يلو في الـ butt، برمز لليلو بالرمز A،
+
+375
+00:28:06,490 --> 00:28:11,530
+لو أتكون لون أحمر في الـ slant، صار في عندي
+
+376
+00:28:11,530 --> 00:28:20,130
+deamination للبيبتون، برمز بالرمز K، هنفهم
+
+377
+00:28:20,130 --> 00:28:25,430
+أكثر لما نقرأ النتائج أو نكتب النتيجة، الـ black
+
+378
+00:28:25,430 --> 00:28:27,630
+precipitate بكتبه H2S،
+
+379
+00:28:35,780 --> 00:28:40,500
+لون الـ media بيكون لون orange، تمام، فبالتالي لو
+
+380
+00:28:40,500 --> 00:28:44,200
+اتحول اللون للون يلو في الـ butt، يبقى هكذا لو صار في
+
+381
+00:28:44,200 --> 00:28:48,440
+عندي glucose fermentation، لو صار في عندي لون أحمر
+
+382
+00:28:48,440 --> 00:28:51,360
+في الـ slant، يبقى صار في عندي deamination للبيبتون،
+
+383
+00:28:51,360 --> 00:28:55,500
+لو اتكون black precipitate، معناه أن في عندي H2S
+
+384
+00:28:55,500 --> 00:28:56,060
+production،
+
+385
+00:29:02,120 --> 00:29:08,300
+هلأ بالنسبة لكيف بيصير أو كيف البكتيريا بتستهلك
+
+386
+00:29:08,300 --> 00:29:13,040
+المواد الغذائية، هلأ كل البكتيريا اللي عندها القدرة
+
+387
+00:29:13,040 --> 00:29:16,820
+أنها تعمل fermentation، هتعمل fermentation لأبسط
+
+388
+00:29:16,820 --> 00:29:21,920
+سكر اللي هو الجلوكوز، يبقى راحت أنا زراعة البكتيريا
+
+389
+00:29:21,920 --> 00:29:26,820
+في high الـ media، أول مصدر رح تلجأ إليه البكتيريا
+
+390
+00:29:26,820 --> 00:29:31,020
+هو الجلوكوز الموجود في الـ media، أكيد هيكون فيه
+
+391
+00:29:31,020 --> 00:29:34,280
+جلوكوز، بما أني بدي أكشف على الـ fermentation، هيكون
+
+392
+00:29:34,280 --> 00:29:38,960
+موجود جلوكوز، لكن موجود بكمية قليلة، يبقى أول مصدر
+
+393
+00:29:38,960 --> 00:29:44,640
+البكتيريا
+
+445
+00:33:21,720 --> 00:33:26,320
+هيتحول نتيجة اللي هو وجود الفيه نار red للون لون
+
+446
+00:33:26,320 --> 00:33:30,880
+الـ media هيتحول للون red في الـ slant فقط في الـ
+
+447
+00:33:30,880 --> 00:33:36,580
+slant قولنا هيتحول لون الـ media للون أحمر لأنه
+
+448
+00:33:36,580 --> 00:33:40,800
+احنا قولنا أن الـ deamination للبيبتون هذا هيصير
+
+449
+00:33:40,800 --> 00:33:46,700
+فقط في ظروف هوائية في الـ aerobic condition فقط
+
+450
+00:33:46,700 --> 00:33:49,780
+هيصير في الـ slant يبقى هيتحول لون الـ slant فقط
+
+451
+00:33:49,780 --> 00:33:55,040
+للون red لكن الـ butt هيظل لونه أصفر يبقى هنحكي
+
+452
+00:33:55,040 --> 00:34:00,120
+لون red أحمر و لون الـ butt لون الـ butt هيظل لون
+
+453
+00:34:00,120 --> 00:34:05,660
+أصفر يبقى red على yellow red قلنا نرمز له بالرمز
+
+454
+00:34:05,660 --> 00:34:11,910
+K يبقى K على A هيك نرمز للنتيجة K على A هذا بالنسبة
+
+455
+00:34:11,910 --> 00:34:16,170
+لو البكتيريا هيك قرأت أو هيك الفحص هيك النتيجة
+
+456
+00:34:16,170 --> 00:34:20,250
+بتكون لو البكتيريا جليكوز fermentation لكن non
+
+457
+00:34:20,250 --> 00:34:23,990
+-lactose fermenter بتكون red على yellow لكن لو
+
+458
+00:34:23,990 --> 00:34:28,050
+كانت جليكوز و lactose fermentation بتكون yellow
+
+459
+00:34:28,050 --> 00:34:33,330
+على yellow على yellow هذا بعد 24 ساعة لكن خلال أول 12
+
+460
+00:34:33,330 --> 00:34:42,970
+ساعة لو كانت lactose أو non-lactose fermenter ستكون
+
+461
+00:34:42,970 --> 00:34:48,130
+A على A عشان هيك نقرأ النتيجة عند 24 ساعة هكذا
+
+462
+00:34:48,130 --> 00:34:53,470
+هنتكلم في هذا الموضوع بالنسبة لو البكتيريا كانت
+
+463
+00:34:53,470 --> 00:34:57,740
+في الأصل non-gelicose fermenter هذا اتكلمنا عنه هذا
+
+464
+00:34:57,740 --> 00:35:00,940
+كله لو كانت البكتيريا في الأصل هي gelicose
+
+465
+00:35:00,940 --> 00:35:04,540
+fermenter لكن لو البكتيريا هي ما عندها القدرة أن
+
+466
+00:35:04,540 --> 00:35:08,320
+تعمل fermentation لأبسط سكر الجليكوز يبقى أكيد مش
+
+467
+00:35:08,320 --> 00:35:12,840
+هتعمل fermentation للسكر الثنائي اللاكتوز والسكراز
+
+468
+00:35:12,840 --> 00:35:17,680
+فبالتالي هتروح لمين؟ هتروح للبيبتون هتعمل له
+
+469
+00:35:17,680 --> 00:35:21,900
+deamination هيتكون عندي alkaline product هيتحول
+
+470
+00:35:21,900 --> 00:35:25,910
+لون الـ media للون red هيصير اللون الـ Red قلنا الـ
+
+471
+00:35:25,910 --> 00:35:30,050
+deamination للبيبتون هيصير في الـ slant في الـ
+
+472
+00:35:30,050 --> 00:35:33,870
+aerobic condition يبقى هيتحول لون الـ slant للون
+
+473
+00:35:33,870 --> 00:35:37,850
+الـ red لكن الـ butt مش هيصله أي تغير No change
+
+474
+00:35:37,850 --> 00:35:41,770
+بقول لي ممكن برضه لو البكتيريا في الأصل non
+
+475
+00:35:41,770 --> 00:35:45,430
+-gelicose fermenter ممكن أنها لو ما عندها القدرة
+
+476
+00:35:45,430 --> 00:35:48,110
+أنها تعمل للـ gelicose فبتعرفش هتعمل للاكتوز ولا
+
+477
+00:35:48,110 --> 00:35:53,880
+للسكراز أكيد هتعمل deamination للبيبتون في الـ
+
+478
+00:35:53,880 --> 00:35:58,380
+aerobic condition في الظروف الهوائية هيتحول لون
+
+479
+00:35:58,380 --> 00:36:02,260
+الـ slant للون red طيب لو البكتيريا ما اكتفت
+
+480
+00:36:02,260 --> 00:36:07,000
+بحاجتها من المواد الغذائية بقول لي هتعمل
+
+481
+00:36:07,000 --> 00:36:11,340
+deamination للبيبتون في ظروف anaerobic condition
+
+482
+00:36:11,340 --> 00:36:17,360
+بشكل اضطراري يبقى هنا صار عندي deamination للبيبتون
+
+483
+00:36:17,360 --> 00:36:22,560
+في ظروف anaerobic condition لكن بشكل اضطراري يبقى
+
+484
+00:36:22,560 --> 00:36:25,440
+لو كانت البكتيريا non-gelicose fermenter ممكن تكون
+
+485
+00:36:25,440 --> 00:36:32,400
+red على no change نكتب K على no change أو red
+
+486
+00:36:32,400 --> 00:36:39,860
+على red اللي هي K على K يبقى هنا النتيجة نكتبها K
+
+487
+00:36:49,430 --> 00:36:54,710
+هنا K على no change لأنه احنا قلنا الـ red نرمز له
+
+488
+00:36:54,710 --> 00:37:14,710
+بالرمز K alkaline تكون وهنا K على K طيب
+
+489
+00:37:15,350 --> 00:37:18,250
+هذا بالنسبة لو كانت البكتيريا non-glycose
+
+490
+00:37:18,250 --> 00:37:22,490
+fermenter إما تكون K على K أو ممكن تكون K على no change
+
+491
+00:37:22,490 --> 00:37:28,610
+وفسرنا كل نتيجة إيش بتكون بالظبط طيب نأخذ
+
+492
+00:37:28,610 --> 00:37:31,970
+عملية الـ fermentation قلنا إما أسيد واتكلمنا أنه
+
+493
+00:37:31,970 --> 00:37:38,310
+بتكون لون yellow في التيوب أو غاز طيب بالنسبة للغاز
+
+494
+00:37:38,310 --> 00:37:43,350
+كيف بدي أكشف عنه؟ قلنا أن الغاز ممكن كنا نكشف عنه
+
+495
+00:37:43,350 --> 00:37:47,690
+عن طريق الدرهام tube لكن الدرهام tube فقط بقدر
+
+496
+00:37:47,690 --> 00:37:52,210
+أحطها لو كانت الـ media broth هاي slant slant agar
+
+497
+00:37:52,210 --> 00:37:55,790
+it's agar بيبقى في عندي نسبة agar ما بقدر أحط درهام
+
+498
+00:37:55,790 --> 00:37:59,850
+tube كيف بدي أكشف على نواتج عملية الـ
+
+499
+00:37:59,850 --> 00:38:03,730
+fermentation لو كان في عندي تكون غاز بقول لي بتكون
+
+500
+00:38:03,730 --> 00:38:07,890
+cracks بتكون زي تشققات في الـ media أو ممكن الـ
+
+501
+00:38:07,890 --> 00:38:12,210
+media تطلع لفوق هيك بعرف أن والله تكون عندي غاز
+
+502
+00:38:12,210 --> 00:38:19,150
+طيب بالنسبة للـ H2S production الـ H2S production
+
+503
+00:38:19,150 --> 00:38:23,650
+احنا قلنا أنه الـ هاي الميديا بتكشف على الـ
+
+504
+00:38:23,650 --> 00:38:27,090
+fermentation بتكشف على الـ deamination للبيبتون
+
+505
+00:38:27,090 --> 00:38:30,410
+قلنا برضه الـ fermentation ممكن يكون acid و غاز
+
+506
+00:38:30,410 --> 00:38:33,950
+وعرفنا كيف كل نتيجة بشوف لونها أو كيف بيكون
+
+507
+00:38:33,950 --> 00:38:39,450
+الشكل لكن بالنسبة للـ H2S production طيب لو تم
+
+508
+00:38:39,450 --> 00:38:43,390
+إنتاج اللون الأسود احنا قلنا الـ H2S production
+
+509
+00:38:43,390 --> 00:38:48,830
+بتكون عندي black precipitate هل هقدر أشوف طبعاً الـ
+
+510
+00:38:48,830 --> 00:38:57,850
+H2S production بيصير زي ما قلنا قبل الـ
+
+511
+00:38:57,850 --> 00:39:01,230
+H2S production قلنا فيه بيصير في ظروف anaerobic
+
+512
+00:39:01,230 --> 00:39:06,620
+condition بيكون في الـ butt صار في عندي إنتاج اللون
+
+513
+00:39:06,620 --> 00:39:11,480
+الأسود هل هقدر أشوف اللون الأصفر الناتج من عملية
+
+514
+00:39:11,480 --> 00:39:14,400
+الـ fermentation خاصة أن الـ fermentation و الـ H2S
+
+515
+00:39:14,400 --> 00:39:17,420
+production بيصيروا في الـ anaerobic condition
+
+516
+00:39:17,420 --> 00:39:22,300
+بيصيروا في الـ butt لو صار في عندي تكون للون الأسود
+
+517
+00:39:22,300 --> 00:39:27,280
+الناتج من أن تكون الـ H2S gas هل هقدر أشوف اللون
+
+518
+00:39:27,280 --> 00:39:33,300
+الأصفر في الـ butt ولا لأ؟ بقول لي لو عملت البكتيريا H2S
+
+519
+00:39:33,300 --> 00:39:39,120
+production بدي أكون عارفة أنه عملت gelicose
+
+520
+00:39:39,120 --> 00:39:43,280
+fermentation يبقى طالما البكتيريا H2S production
+
+521
+00:39:43,280 --> 00:39:46,760
+positive يبقى هي gelicose fermentation positive
+
+522
+00:39:46,760 --> 00:39:53,020
+لأن إنتاج الـ H2S production يحصل في الوسط الحمضي
+
+523
+00:39:53,280 --> 00:39:58,480
+الناتج من عملية التخمير اللي هو السكريات فبالتالي و
+
+524
+00:39:58,480 --> 00:40:01,900
+اتكون acid صار في عندي acid condition هيتكون الـ
+
+525
+00:40:01,900 --> 00:40:07,200
+H2S production يبقى بدنا نكون عارفين إذا اتكون عند
+
+526
+00:40:07,200 --> 00:40:11,160
+الـ H2S لون اتكون لون أسود في الـ butt على طول بكتب
+
+527
+00:40:11,160 --> 00:40:16,240
+جنبه أن صار في عندي جليكوز fermentation لأن عملية
+
+528
+00:40:16,240 --> 00:40:21,260
+الـ H2S production بتصير في الوسط الحمضي هذا
+
+529
+00:40:21,260 --> 00:40:22,180
+بالنسبة ل
+
+530
+00:40:26,150 --> 00:40:30,810
+الـ H2S production يبقى عارفة أن كل عملية عشان
+
+531
+00:40:30,810 --> 00:40:33,610
+نتيجة deamination للبيبتون قولنا بيكون في عندي
+
+532
+00:40:33,610 --> 00:40:37,830
+تكون لون red في الـ slant ممكن يصير بشكل اضطراري
+
+533
+00:40:37,830 --> 00:40:42,690
+في الـ butt الـ fermentation لو صار في عندي لجليكوز فقط
+
+534
+00:40:42,690 --> 00:40:46,830
+جليكوز fermentation بدون ما يصير للاكتوز والسكراز
+
+535
+00:40:46,830 --> 00:40:51,890
+بتكون عندي في الـ butt لون أصفر لكن في الـ slant بيكون
+
+536
+00:40:51,890 --> 00:40:56,000
+في عندي لون أحمر نتيجة الـ deamination للـ peptide
+
+537
+00:40:56,000 --> 00:40:58,760
+لكن لو البكتيريا عندها القدرة أنها تعمل
+
+538
+00:40:58,760 --> 00:41:01,180
+fermentation لكل السكريات الموجودة في الـ media
+
+539
+00:41:01,180 --> 00:41:05,180
+هلاقي كل tube لونه أصفر الـ H2S acid production
+
+540
+00:41:05,180 --> 00:41:10,660
+قولنا لو اتكون راسب أسود في الـ butt يبقى هذا معناه
+
+541
+00:41:10,660 --> 00:41:13,320
+أنه بدي أعرف أنه والله صار في عندي جليكوز
+
+542
+00:41:13,320 --> 00:41:17,500
+fermentation already لأن الـ H2S acid production
+
+543
+00:41:17,500 --> 00:41:19,280
+بيصير في الـ acidic condition
+
+544
+00:41:25,030 --> 00:41:32,450
+طيب فيه معلومة مهمة أن أنا بقرأ النتائج للـ triple
+
+545
+00:41:32,450 --> 00:41:38,910
+sugar iron agar عند 24 ساعة ممنوع أقرأ بعد 24 ساعة
+
+546
+00:41:38,910 --> 00:41:45,270
+و ممنوع أقرأ قبل 24 ساعة طيب لو قرأت بعد 24 ساعة
+
+547
+00:41:45,270 --> 00:41:49,110
+بقول لي كل الكربوهيدرات الموجودة في الـ media هتكون
+
+548
+00:41:49,110 --> 00:41:55,810
+تم استهلاكها will be depleted البكتيريا لو برضه
+
+549
+00:41:55,810 --> 00:42:00,210
+البكتيريا هتلجأ بما أنه خلصت بعد 24 ساعة هيكون خلص
+
+550
+00:42:00,210 --> 00:42:03,910
+الجليكوز already هتكون خلص اللاكتوز والسكراز
+
+551
+00:42:03,910 --> 00:42:08,030
+البكتيريا هتلجأ لمصادر ثانية اللي هو البيبتون
+
+552
+00:42:08,030 --> 00:42:14,010
+وهتعمله deamination فبالتالي لو كانت النتيجة A
+
+553
+00:42:14,010 --> 00:42:17,850
+على A لو كانت البكتيريا في الأصل جليكوز fermenter
+
+554
+00:42:17,850 --> 00:42:21,130
+عندها القدرة على تخمير اللاكتوز والسكراز قلنا
+
+555
+00:42:21,130 --> 00:42:25,220
+بتكون النتيجة A على A بتعمل fermentation للجليكوز
+
+556
+00:42:25,220 --> 00:42:30,120
+خلال 12 ساعة خلال أول 12 ساعة بعد هيخلص الجليكوز
+
+557
+00:42:30,120 --> 00:42:33,160
+لأنه موجود في كمية قليلة هتروح للكتوز والسكراز
+
+558
+00:42:33,160 --> 00:42:37,320
+هينتج كمية أسيد كبيرة هينتشر في كل tube اللون
+
+559
+00:42:37,320 --> 00:42:41,140
+الأصفر وهيتكون yellow في الـ slant و yellow في الـ butt
+
+560
+00:42:41,140 --> 00:42:45,680
+يبقى هتكون النتيجة A على A لو كانت النتيجة A على A
+
+561
+00:42:45,680 --> 00:42:50,950
+تمام هي في الأصل lactose fermenter ورحت أنا قرأت
+
+562
+00:42:50,950 --> 00:42:55,570
+النتيجة بعد 24 ساعة بقول لي أنه أكيد بعد 24 ساعة
+
+563
+00:42:55,570 --> 00:43:00,510
+هيخلص اللاكتوز والسكراز هتروح البكتيريا لمصدر ثاني
+
+564
+00:43:00,510 --> 00:43:03,750
+اللي هو البيبتون هتعمله deamination الـ
+
+565
+00:43:03,750 --> 00:43:06,770
+deamination للبيبتون قلنا بيصير في ظروف aerobic
+
+566
+00:43:06,770 --> 00:43:11,830
+condition بيصير في الـ slant بيتحول لون الـ slant
+
+567
+00:43:11,830 --> 00:43:17,210
+للون red يبقى هتتحول النتيجة الـ A على A لـ K على A
+
+568
+00:43:17,210 --> 00:43:22,290
+فهفكرها أنا أنها هي non-lactose fermenter لكن هي في
+
+569
+00:43:22,290 --> 00:43:25,510
+الأصل lactose fermenter لكن لأني أنا قرأت النتيجة
+
+570
+00:43:25,510 --> 00:43:32,790
+بعد 24 ساعة طيب لو قرأت قبل 24 ساعة بقول لي لو قرأتِ
+
+571
+00:43:32,790 --> 00:43:36,130
+على سبيل المثال كانت البكتيريا هي non-lactose or
+
+572
+00:43:36,130 --> 00:43:40,310
+saccharose fermenter كانت K على A ورحت أنا قرأتها
+
+573
+00:43:40,670 --> 00:43:45,010
+قبل 24 ساعة قبل 24 ساعة قلنا سواء كانت lactose
+
+574
+00:43:45,010 --> 00:43:47,970
+fermenter أو non-lactose fermenter بتكون A على A
+
+575
+00:43:47,970 --> 00:43:51,790
+لأنه فقط بيتم استهلاك الجليكوز خلال أول 12 ساعة
+
+576
+00:43:51,790 --> 00:43:57,250
+بيتحول بيتكون acid هذه نواتج عملية الـ
+
+577
+00:43:57,250 --> 00:44:00,310
+fermentation acid بيصير في الـ anaerobic condition
+
+578
+00:44:00,310 --> 00:44:06,190
+لكن اللون الأصفر بيمتد في كل tube فبالتالي هفكر
+
+579
+00:44:06,190 --> 00:44:12,110
+النتيجة A على A يبقى لو أنا قرأت قبل 24 ساعة وكانت
+
+580
+00:44:12,110 --> 00:44:15,390
+البكتيريا في الأصل هي non-lactose fermenter يبقى
+
+581
+00:44:15,390 --> 00:44:19,630
+النتيجة المفترض أن أقرأها K على A لو قرأتها قبل
+
+582
+00:44:19,630 --> 00:44:23,610
+24 ساعة هتكون النتيجة A على A فبالتالي هيصير في
+
+583
+00:44:23,610 --> 00:44:27,050
+عندي false result عشان هيك لازم أقرأ النتيجة عند
+
+584
+00:44:27,050 --> 00:44:33,070
+24 ساعة مش قبل 24 ساعة و مش بعد 24 ساعة قلنا بعد
+
+585
+00:44:33,070 --> 00:44:37,490
+24 ساعة لو كانت البكتيريا A على A هتبين عندي كأنها
+
+586
+00:44:37,490 --> 00:44:42,800
+K على A لكن لو قرأت قبل 24 ساعة و كانت في الأصل هي
+
+587
+00:44:42,800 --> 00:44:46,760
+K على A هتبين عندي كأنها A على A فبالتالي كل
+
+588
+00:44:46,760 --> 00:44:48,880
+النتائج هتكون عندي غلط
+
+589
+00:44:54,580 --> 00:44:58,900
+نرجع على السلايدات Kliger iron agar اللي Kliger هو
+
+590
+00:44:58,900 --> 00:45:02,360
+اسم العالم iron لأنه بدي أكشف فيها على الـ H2S
+
+591
+00:45:02,360 --> 00:45:06,880
+production agar لأنه أكيد في الـ media هي فيها نسبة
+
+592
+00:45:06,880 --> 00:45:11,360
+agar بنسبة واحد ونصف في المية الـ triple sugar iron
+
+593
+00:45:11,360 --> 00:45:15,280
+agar لأنه الـ triple فيها ثلاثة سكريات قولنا الـ
+
+594
+00:45:15,280 --> 00:45:20,080
+dextrose اللي هو الجليكوز أو اللاكتوز والسكروز Iron
+
+595
+00:45:20,080 --> 00:45:23,460
+بدي أكشف على الـ H2S production و الـ agar لأن فيها
+
+596
+00:45:23,460 --> 00:45:27,600
+نسبة agar واحد و نصف في الميديا واحد و نصف بالمئة
+
+597
+00:45:27,600 --> 00:45:31,780
+Kliger iron agar and triple sugar iron agar are
+
+598
+00:45:31,780 --> 00:45:36,140
+widely used in the identification of gram negative
+
+599
+00:45:36,140 --> 00:45:40,500
+bacteria particularly the Enterobacteriaceae هذه
+
+600
+00:45:40,500 --> 00:45:45,420
+الميديا بتستخدم للتعريف للـ gram negative bacteria
+
+601
+00:45:45,420 --> 00:45:48,230
+خاصة الـ Enterobacteriaceae قلنا أن الـ
+
+602
+00:45:48,230 --> 00:45:51,570
+Enterobacteriaceae هي الـ gram negative بس  تعتبر
+
+603
+00:45:51,570 --> 00:45:56,670
+enteric بكتيريا بكشف من خلال أو بعمل تعريف للـ
+
+604
+00:45:56,670 --> 00:45:59,590
+Enterobacteriaceae من خلال triple sugar iron agar
+
+605
+00:45:59,590 --> 00:46:03,870
+أو Kliger iron agar هذه الـ media the media are
+
+606
+00:46:03,870 --> 00:46:08,150
+identical الميديتين متشابهة نفس الشيء متشابهة
+
+607
+00:46:08,150 --> 00:46:11,710
+لكن الاختلاف أن الـ triple sugar iron agar بتحتوي
+
+608
+00:46:11,710 --> 00:46:15,970
+على السكروز والاختلاف بالإضافة للـ dextrose والـ
+
+609
+00:46:15,970 --> 00:46:20,930
+lactose الموجود في الـ Kliger iron agar كيف أنا
+
+610
+00:46:20,930 --> 00:46:25,170
+بصبها الـ media قلنا الـ media بصبها slant تمام كيف
+
+611
+00:46:25,170 --> 00:46:29,630
+بزرعها بعمل stabbing للـ butt للـ butt بعمل stabbing
+
+612
+00:46:29,630 --> 00:46:33,310
+بعدين بعمل streaking على الـ slant surface وبعمل زي
+
+613
+00:46:33,310 --> 00:46:37,530
+زجاج هكذا احنا قلنا أن نزرع الـ slant tube هكذا
+
+614
+00:46:37,530 --> 00:46:41,470
+البكتيريا بتتعرض لـ anaerobic environment تقول لنا
+
+615
+00:46:41,470 --> 00:46:45,890
+بتتعرض لظروف لا هوائية من خلال الـ butt وظروف هي
+
+616
+00:46:45,890 --> 00:46:50,090
+هوائية اللي هي الـ aerobic من خلال الـ slant النار
+
+617
+00:46:50,090 --> 00:46:53,870
+red موجود كـ indicator كـ phenol red indicator في الـ media
+
+618
+00:46:53,870 --> 00:46:57,
+
+667
+00:50:19,050 --> 00:50:22,030
+عنه من خلال وجود الـ phenol red اللي هو مؤشر  pH
+
+668
+00:50:22,030 --> 00:50:25,130
+السوديوم سيوسالفات هو الـ substrate الذي
+
+669
+00:50:25,130 --> 00:50:29,550
+سيشتغل عليه الانزيم  سوف يعمل لو موجود فيه البكتيريا
+
+670
+00:50:29,550 --> 00:50:33,630
+سيُحوّله إلى hydrogen sulfide  الـ hydrogen
+
+671
+00:50:33,630 --> 00:50:38,430
+sulfide سيتفاعل مع الـ iron salt فبالتالي سيتكون
+
+672
+00:50:38,430 --> 00:50:42,600
+عندي black precipitate كما ذكرناه و شرحناه في
+
+673
+00:50:42,600 --> 00:50:46,720
+ميديا SIM Ferric Ammonium Citrate هو مؤشر H2S
+
+674
+00:50:46,720 --> 00:50:50,080
+الذي سيرتبط مع غاز H2S  وسيعطينا
+
+675
+00:50:50,080 --> 00:50:53,580
+black precipitate. أي ميديا تحتوي على
+
+676
+00:50:53,580 --> 00:50:56,520
+Sodium Chloride  للحفاظ على الضغط الأسموزي
+
+677
+00:50:56,520 --> 00:51:03,840
+Osmotic Pressure.  الأجار يعتبر عامل مُتماسك solidifying agent هذه
+
+678
+00:51:03,840 --> 00:51:09,180
+الميديا تُستخدم في تعريف وتشخيص Antirobacteriasi
+
+679
+00:51:09,180 --> 00:51:13,360
+من خلال هذه الميديا نُشخص قدرة البكتيريا على
+
+680
+00:51:13,360 --> 00:51:19,300
+إنتاج أو تكوين غاز نتيجة تخمّر السكر fermentation
+
+681
+00:51:19,300 --> 00:51:24,380
+تخمير اللاكتوز أو السكاروز أيضاً fermentation
+
+682
+00:51:24,380 --> 00:51:28,140
+إنتاج غاز hydrogen sulfide  H2S
+
+683
+00:51:33,190 --> 00:51:37,110
+مبدأ الكائنات المخمرة jelly cause is
+
+684
+00:51:37,110 --> 00:51:41,670
+أول سكر يُحَلَل catabolized في هذه الـ media
+
+685
+00:51:41,670 --> 00:51:45,210
+أنا أُشخص التخمّر. قلنا أن في هذه
+
+686
+00:51:45,210 --> 00:51:48,950
+الـ media أُشخص التخمّر الآن.
+
+687
+00:51:48,950 --> 00:51:54,310
+البكتيريا ستستهلك, ستلجأ إلى أبسط الطرق
+
+688
+00:51:54,310 --> 00:51:59,570
+للحصول على الطاقة والغذاء,  ستبدأ بأبسط مركب
+
+689
+00:51:59,570 --> 00:52:02,730
+وهو الجلوكوز, ستبدأ بالـ Dextrose الذي
+
+690
+00:52:02,730 --> 00:52:07,890
+قلنا أنه سكر بسيط simple sugar. ستُخَمّره لو
+
+691
+00:52:07,890 --> 00:52:10,690
+البكتيريا لديها القدرة على تخمير هذا
+
+692
+00:52:10,690 --> 00:52:14,990
+الجلوكوز, سيتكون عندي حمض acid
+
+693
+00:52:14,990 --> 00:52:20,470
+فبالتالي سيتحول لون الـ media للون أصفر. If glycose
+
+694
+00:52:20,470 --> 00:52:24,130
+is used up. إذا تم استهلاك الجلوكوز, البكتيريا
+
+695
+00:52:24,130 --> 00:52:27,230
+ستصل إلى نقطة مفترق استقلابي metabolic fork in the rod. البكتيريا ستصل
+
+696
+00:52:27,230 --> 00:52:33,770
+إلى لحظة التشعب في المسار تمام هذا
+
+697
+00:52:33,770 --> 00:52:37,130
+بالنسبة لأول شيء، قلنا أن البكتيريا ستلجأ
+
+698
+00:52:37,130 --> 00:52:43,430
+إلى أول مصدر وهو الجلوكوز, ستستهلك الجلوكوز, سيتكون
+
+699
+00:52:43,430 --> 00:52:47,870
+حمض، سيتحول لون الـ media للون أصفر وسينتشر أيضاً في
+
+700
+00:52:47,870 --> 00:52:51,990
+الـ slant للون أصفر yellow. If an organism produces the
+
+701
+00:52:51,990 --> 00:52:56,550
+enzyme to hydrolyze this saccharide lactose, the
+
+702
+00:52:56,550 --> 00:53:01,050
+organism will continue fermenting to assist in ATP
+
+703
+00:53:01,050 --> 00:53:06,320
+generation. أقول لكم، لو أن البكتيريا, لو نفذ طبعاً  أكيد
+
+704
+00:53:06,320 --> 00:53:10,580
+الجلوكوز موجود بكمية قليلة, لو نفذ الـ dextrose
+
+705
+00:53:10,580 --> 00:53:14,980
+ستستهلك اللاكتوز أو السكاروز أو كليهما معاً
+
+706
+00:53:14,980 --> 00:53:19,120
+حسب الانزيمات الموجودة عند البكتيريا، تمام هذا
+
+707
+00:53:19,120 --> 00:53:26,140
+بالنسبة للّكتوز والسكاروز. However, if the glucose
+
+708
+00:53:26,140 --> 00:53:29,440
+is limiting and the organism doesn't produce the
+
+709
+00:53:29,720 --> 00:53:32,940
+necessary enzyme to catabolize lactose, the
+
+710
+00:53:32,940 --> 00:53:36,780
+organism can utilize the protein in the media via
+
+711
+00:53:36,780 --> 00:53:41,680
+the process of deamination. أقول لكم لو الجلوكوز نفذ
+
+712
+00:53:41,680 --> 00:53:44,740
+والبكتيريا لا تمتلك القدرة على تحليل إنزيمات
+
+713
+00:53:44,740 --> 00:53:47,280
+اللاكتوز، يعني لو البكتيريا في الأصل تُخَمِّر الجلوكوز
+
+714
+00:53:47,280 --> 00:53:49,360
+fermentation لكن لا تمتلك القدرة على
+
+715
+00:53:49,360 --> 00:53:53,260
+تخمير اللاكتوز ولا السكاروز, بالتأكيد ستستهلك
+
+716
+00:53:53,260 --> 00:53:57,340
+البروتين (البيبتون) الموجود في الميديا ستُجري عملية
+
+717
+00:53:57,340 --> 00:54:01,480
+deamination  وسيتحول لون الـ slant للون  ذاك،  يبقى هذا
+
+718
+00:54:01,480 --> 00:54:07,760
+بالنسبة للمبدأ الذي شرحناه سابقاً  بالنسبة
+
+719
+00:54:07,760 --> 00:54:10,920
+للخطوات قلنا نُجري زراعة على triple sugar iron
+
+720
+00:54:10,920 --> 00:54:14,120
+agar slant  من خلال إجراء عملية طعن للبطّ و
+
+721
+00:54:14,120 --> 00:54:17,880
+الـ slant بزرعه بشكل متعرج، نقوم بعملية حضانة لمدة 24
+
+722
+00:54:17,880 --> 00:54:23,330
+ساعة. ممنوع قبلها، ممنوع بعدها. قلنا أن التوقيت
+
+723
+00:54:23,330 --> 00:54:27,690
+حاسم  في قراءةtriple sugar iron agar أو
+
+724
+00:54:27,690 --> 00:54:32,310
+clagar iron agar. لو قرأت قبل 24 ساعة، an
+
+725
+00:54:32,310 --> 00:54:36,650
+early reading could reveal yellow throughout the
+
+726
+00:54:36,650 --> 00:54:40,930
+media. أقول لكم، لو قرأت قبل 24 ساعة يكون قد تم
+
+727
+00:54:40,930 --> 00:54:45,930
+استهلاك الجلوكوز فقط، التخمّر fermentation  سيتحول اللون
+
+728
+00:54:45,930 --> 00:54:50,260
+الوسط للون أصفر yellow، فبالتالي هذا سيحدث في الظروف اللاهوائية
+
+729
+00:54:50,260 --> 00:54:53,240
+Un-aerobic condition ويمتد اللون الأصفر في كل الـ tube
+
+730
+00:54:53,240 --> 00:54:56,980
+فبالتالي كل الـ media سيكون لونها أصفر،  هذا يدفع
+
+731
+00:54:56,980 --> 00:55:00,900
+leading one to conclude that the organism is a
+
+732
+00:55:00,900 --> 00:55:05,180
+lactose fermenter عندما قد لا يكون قد قام  بذلك  بعد
+
+733
+00:55:05,180 --> 00:55:09,820
+exercised the glucose. أقول لكم سأعتقد أن الكائن
+
+734
+00:55:09,820 --> 00:55:15,110
+organism قد قام بعملية تخمّر اللاكتوز fermentation
+
+735
+00:55:15,110 --> 00:55:20,550
+لكنّه حتى الآن استهلك الجلوكوز فقط. لو قرأت
+
+736
+00:55:20,550 --> 00:55:25,610
+بعد 24 ساعة، قراءة بعد، لو قرأت بعد 24
+
+737
+00:55:25,610 --> 00:55:28,910
+ساعة the lactose has been depleted. أقول لكم أن اللاكتوز
+
+738
+00:55:28,910 --> 00:55:34,220
+قد تم استهلاكه بالكامل.  could reveal a yellow butt
+
+739
+00:55:34,220 --> 00:55:37,620
+and red slant, leading one to falsely conclude that
+
+740
+00:55:37,620 --> 00:55:40,740
+the organism is glucose only fermenter. لو قرأت
+
+741
+00:55:40,740 --> 00:55:45,240
+بعد 24 ساعة، اللاكتوز والسكاروز سيتم استهلاكهما
+
+742
+00:55:45,240 --> 00:55:48,120
+فبالتالي البكتيريا ستلجأ إلى مصادر أخرى وهي
+
+743
+00:55:48,120 --> 00:55:51,560
+البيبتون، فبالتالي سيكون لديّ deamination للبيبتون
+
+744
+00:55:51,560 --> 00:55:55,060
+في الـ slant، سيتحول لون الـ slant للون أحمر، لكن في
+
+745
+00:55:55,060 --> 00:55:59,140
+الـ butt سيكون أصفر yellow، فبالتالي سأفكر هذه النتيجة
+
+746
+00:55:59,140 --> 00:56:04,450
+على أنها فقط البكتيريا لديها القدرة على
+
+747
+00:56:04,450 --> 00:56:08,870
+تخمير الجلوكوز وهي غير مُخَمِّرة للّكتوز non-lactose fermenter
+
+748
+00:56:08,870 --> 00:56:14,330
+فهذا سيؤدي إلى نتائج خاطئة falsely result  بالنسبة للنتائج
+
+749
+00:56:14,330 --> 00:56:20,650
+لو كان لون الـ butt أصفر yellow  في الـ slant أصفر yellow  يبقى
+
+750
+00:56:20,650 --> 00:56:24,430
+سأعرف أن البكتيريا قد خَمَّرَت الجلوكوز واللاكتوز
+
+751
+00:56:24,430 --> 00:56:29,360
+والسكاروز fermentation. سيتكون عندي حمض  هذا الحمض
+
+752
+00:56:29,360 --> 00:56:34,380
+acid حوّل لون الوسط إلى اللون الأصفر نتيجة وجود الفينول
+
+753
+00:56:34,380 --> 00:56:38,000
+red. انتشر في الـ slant وفي الـ butt، أرمز له
+
+754
+00:56:38,000 --> 00:56:43,060
+بالرمز A على A، تمام هذا بالنسبة لأول رمز، هذا لو
+
+755
+00:56:43,060 --> 00:56:48,640
+قرأت النتائج عند 24 ساعة، لأنه لو قرأت قبل عند
+
+756
+00:56:48,640 --> 00:56:52,480
+أول 12 ساعة، يعني أن فقط حدث استهلاك
+
+757
+00:56:52,480 --> 00:56:57,340
+للجلوكوز، هذا كله نتحدث عند 24 ساعة. طيب، لو لاحظت في
+
+758
+00:56:57,340 --> 00:57:00,580
+الـ butt  هناك لون أصفر yellow لكن في الـ slant  هناك لون أحمر red
+
+759
+00:57:00,580 --> 00:57:03,820
+في الـ butt أصفر yellow، يعني أن هناك فقط
+
+760
+00:57:03,820 --> 00:57:07,560
+تخمّر جلوكوزي gelatinous fermentation، تكون حمض في
+
+761
+00:57:07,560 --> 00:57:10,820
+الـ slant، تحوّل إلى أحمر red، يبقى هناك deamination
+
+762
+00:57:10,820 --> 00:57:15,340
+للبيبتون بشكل هوائي، في ظروف هوائية aerobic
+
+763
+00:57:15,340 --> 00:57:21,080
+condition، تمام، الرمز بما أنه أصفر yellow نضع A، بما أنه
+
+764
+00:57:21,080 --> 00:57:26,070
+أحمر red، نضع K. لم يحدث
+
+765
+00:57:26,070 --> 00:57:29,570
+تخمّر fermentation، لم أرَ لون أحمر red في الـ butt أو
+
+766
+00:57:29,570 --> 00:57:33,030
+في الـ slant، لم يحدث، لم أرَ لون
+
+767
+00:57:33,030 --> 00:57:38,150
+أصفر yellow، لم يحدث تخمّر fermentation، ماذا يعني
+
+768
+00:57:38,150 --> 00:57:40,430
+لون أحمر red في الـ slant؟  يعني أن هناك
+
+769
+00:57:40,430 --> 00:57:44,310
+deamination للبيبتون في الظروف الهوائية aerobic
+
+770
+00:57:44,310 --> 00:57:49,370
+condition. البكتيريا لم تجد ما تحتاجه من
+
+771
+00:57:49,370 --> 00:57:52,810
+المواد الغذائية، قامت بعملية deamination  للبيبتون بشكل
+
+772
+00:57:52,810 --> 00:57:56,890
+اضطرارا في الظروف اللاهوائية anaerobic condition، يبقى هناك
+
+773
+00:57:56,890 --> 00:58:00,010
+deamination للبيبتون aerobically and
+
+774
+00:58:00,010 --> 00:58:02,790
+anaerobically. تكون منتجات قلوية alkaline product
+
+775
+00:58:02,790 --> 00:58:07,410
+فتغيّر لون الـ media للون أحمر نتيجة تكون
+
+776
+00:58:07,410 --> 00:58:12,130
+المواد القلوية alkaline products. يبقى الرمز K على K. هذه البكتيريا
+
+777
+00:58:12,130 --> 00:58:15,430
+بما أنها في الأصل غير مُخَمِّرة، يبقى هي ليست من
+
+778
+00:58:15,430 --> 00:58:19,290
+الـ Enterobacteriaceae لأن الـ Enterobacteriaceae  تقوم بعملية
+
+779
+00:58:19,290 --> 00:58:23,810
+تخمّر الجلوكوز fermentation. كلها لو الـ red كان في الـ slant لكن
+
+780
+00:58:23,810 --> 00:58:27,370
+في الـ butt لم يحدث أي تغيير، يبقى هنا أقول أن
+
+781
+00:58:27,370 --> 00:58:30,530
+لم يحدث تخمّر fermentation، لكن حدثت عملية
+
+782
+00:58:30,530 --> 00:58:34,190
+deamination للبيبتون لكن فقط في الظروف الهوائية aerobic condition
+
+783
+00:58:34,190 --> 00:58:37,670
+تكون منتجات قلوية alkaline product تحوّل لون الـ media أو
+
+784
+00:58:37,670 --> 00:58:41,670
+لون الـ slant فقط إلى اللون الأحمر. بما أنه لم يحدث
+
+785
+00:58:41,670 --> 00:58:44,030
+تخمّر fermentation يبقى ليست من الـ Enterobacteria
+
+786
+00:58:44,030 --> 00:58:48,410
+سنرمز لها بالرمز K على no change. لو لم
+
+787
+00:58:48,410 --> 00:58:53,510
+يحدث أي تغيير في الـ slant أو في الـ butt، يعني إما
+
+788
+00:58:53,510 --> 00:58:59,050
+أن البكتيريا تنمو ببطء أو أنني لم أزرع
+
+789
+00:58:59,050 --> 00:59:01,970
+بكتيريا، هي أصلاً ليست من عائلة الـ Enterobacteriaceae
+
+790
+00:59:01,970 --> 00:59:06,990
+لأنّ البكتيريا، كلّ الـ Enterobacteriaceae
+
+791
+00:59:06,990 --> 00:59:10,950
+تنمو عادياً عند 24 ساعة، تكون قد نمت، فبالتالي not
+
+792
+00:59:10,950 --> 00:59:13,750
+change على not change، أقول أنها ليست من
+
+793
+00:59:13,750 --> 00:59:17,250
+Enterobacteriaceae. قلنا لـ Black precipitates نرمز
+
+794
+00:59:17,250 --> 00:59:23,030
+لها بالرمز H2S. اختزال الكبريت Sulphur Reduction أو ما يسمى
+
+795
+00:59:23,030 --> 00:59:25,430
+عندما يحدث اختزال الكبريت Reduction، قلنا
+
+796
+00:59:25,430 --> 00:59:28,310
+هذا يحدث في الظروف الحمضية Acidic Condition الناتج من
+
+797
+00:59:28,310 --> 00:59:31,670
+تخمّر الجلوكوز أو اللاكتوز أو السكاروز
+
+798
+00:59:31,670 --> 00:59:35,590
+فبالتالي لو تكون اللون الأسود يجب أن أكتب بجانبها
+
+799
+00:59:35,590 --> 00:59:41,170
+A  الذي في الـ butt أضع بجانبه A،  ممنوع أن أضع K
+
+800
+00:59:41,170 --> 00:59:45,410
+لأنّ H2S  يحدث اختزاله في ظروف حمضية acidic
+
+801
+00:59:45,410 --> 00:59:50,110
+condition، يبقى ممنوع أن أرى نتيجة K على K مع الـ H2S
+
+802
+00:59:50,110 --> 00:59:53,750
+لأنّ الـ H2S يحدث في ظروف حمضية acidic condition، يبقى من
+
+803
+00:59:53,750 --> 00:59:59,760
+حتى لا أرى الـ H2S أضع في الـ butt A. لو تكوّن
+
+804
+00:59:59,760 --> 01:00:03,560
+هناك تشققات في الـ media أعرف أنّه ناتج
+
+805
+01:00:03,560 --> 01:00:08,120
+عن عملية تخمّر fermentation  حمضية مع غاز gas، فأضيف رمز
+
+806
+01:00:08,120 --> 01:00:14,020
+الغاز gas بجانب الرمز، A على A مثلاً أو K على
+
+807
+01:00:14,020 --> 01:00:18,180
+A، أضع رمزاً بجانب الرمز  يعني أنّه حدث
+
+808
+01:00:18,180 --> 01:00:21,720
+من نواتج عملية التخمّر fermentation أيضاً حمض acid
+
+809
+01:00:22,270 --> 01:00:28,310
+هذا بالنسبة لكلّ الأشياء المحتملة، أول
+
+810
+01:00:28,310 --> 01:00:31,790
+رمزين هذا الرمز وهذا الرمز يعني أنّها من عائلة
+
+811
+01:00:31,790 --> 01:00:35,850
+الـ Enterobacteriaceae، لكن هذه الثلاثة ليست من عائلة
+
+812
+01:00:35,850 --> 01:00:38,510
+الـ Enterobacteriaceae، هذان الاثنان من عائلة الـ
+
+813
+01:00:38,510 --> 01:00:42,270
+Enterobacteriaceae، هذه مُخَمِّرة للّكتوز lactose fermenter، هذه غير مُخَمِّرة
+
+814
+01:00:42,270 --> 01:00:45,550
+للاكتوز non-lactose fermenter مثل البروتيوس والسلمونيلا والشيجيلة
+
+815
+01:00:45,550 --> 01:00:49,710
+لكن هذه مُخَمِّرة للّكتوز lactose fermenter  مثل الكوليفورم
+
+816
+01:00:50,090 --> 01:00:54,650
+هذه الثلاثة ليست من عائلة الـ Enterobacteriaceae،  طيب
+
+817
+01:00:54,650 --> 01:01:01,690
+نأتي للنتائج، أول واحدة أقول أنّ رمزها K على A
+
+818
+01:01:01,690 --> 01:01:05,530
+تمام، لديّ لون، تكوّن لون أصفر yellow وتكوّن لون أحمر red
+
+819
+01:01:05,530 --> 01:01:09,010
+يبقى K على A، يبقى هي من عائلة الـ
+
+820
+01:01:09,010 --> 01:01:12,030
+Enterobacteriaceae التي هي غير مُخَمِّرة للّكتوز non-lactose fermenter مثل
+
+821
+01:01:12,030 --> 01:01:16,050
+المرجانيلا  المرجاني.  لم يتكوّن غاز gas يبقى
+
+822
+01:01:16,050 --> 01:01:21,420
+K على A، هذه البكتيريا. K على no change. إذا
+
+823
+01:01:21,420 --> 01:01:25,780
+لاحظتم لون الـ media هنا  كـ control، هذه
+
+824
+01:01:25,780 --> 01:01:28,760
+غير مزروعة كـ control، لون الـ media هكذا يكون لونه برتقالي
+
+825
+01:01:28,760 --> 01:01:32,600
+orange. نلاحظ أنّ في الـ slant تكوّن لون أحمر red، لكن في
+
+826
+01:01:32,600 --> 01:01:37,040
+الـ butt كان نفس اللون، هذا لم يتغير no change يبقى هذه
+
+827
+01:01:37,040 --> 01:01:40,700
+البكتيريا المعوية الزائفة Pseudomonas aeruginosa، يبقى هذا الرمز، ليست من عائلة
+
+828
+01:01:40,700 --> 01:01:43,640
+الـ Enterobacteriaceae، وفعلاً البكتيريا المعوية الزائفة  ليست
+
+829
+01:01:43,640 --> 01:01:46,900
+من عائلة الـ Enterobacteriaceae، هذه غير مزروعة كـ
+
+830
+01:01:46,900 --> 01:01:51,910
+control.  يمكن أن يحدث إنتاج H2S production قلنا
+
+831
+01:01:51,910 --> 01:01:56,810
+من  أرى H2S أضع بجانبه A لأنّ H2S
+
+832
+01:01:56,810 --> 01:01:59,970
+production يحدث في ظروف حمضية acid condition، يبقى أكيد
+
+833
+01:01:59,970 --> 01:02:05,210
+يجب أن يكون هناك تخمّر جلوكوزي glucose fermentation  قبل تكوّن اللون
+
+834
+01:02:05,210 --> 01:02:10,010
+الأحمر red، يبقى أقول K على A مع الـ H2S، K على A يبقى هي
+
+835
+01:02:10,010 --> 01:02:14,130
+من عائلة الـ Enterobacteriaceae، من عائلة الـ غير
+
+836
+01:02:14,130 --> 01:02:18,050
+مُخَمِّرة للّكتوز non-lactose fermenter لأنها K على A. K على A، هي غير
+
+837
+01:02:18,050 --> 01:02:20,770
+مُخَمِّرة للّكتوز non-lactose fermentation،  تكوّن عندي إنتاج H
+
+889
+01:06:45,470 --> 01:06:51,050
+اللي هي من الـ Non-Collifer هذا بالنسبة للـ S
+
+890
+01:06:51,050 --> 01:06:54,850
+اللي هو Triple Sugar Iron Agar هكذا نكون خلصنا أول
+
+891
+01:06:54,850 --> 01:06:58,890
+ميديتين الـ SIM و Triple Sugar Iron Agar أو
+
+892
+01:06:58,890 --> 01:07:02,810
+Triple Sugar Iron Agar
+
diff --git a/PL9fwy3NUQKwZWgAb_V1ATlRRsH-6i3cPC/owBu12fuBog_postprocess.srt b/PL9fwy3NUQKwZWgAb_V1ATlRRsH-6i3cPC/owBu12fuBog_postprocess.srt
new file mode 100644
index 0000000000000000000000000000000000000000..038f6ebd4421903e3a32d2aa4dfab2a1cb537988
--- /dev/null
+++ b/PL9fwy3NUQKwZWgAb_V1ATlRRsH-6i3cPC/owBu12fuBog_postprocess.srt
@@ -0,0 +1,3568 @@
+1
+00:00:03,170 --> 00:00:07,030
+السلام عليكم ورحمة الله وبركاته يعطيكم العافية
+
+2
+00:00:07,030 --> 00:00:11,070
+جميعا نستكمل معكم سلسلة محاضرات مساق الـ
+
+3
+00:00:11,070 --> 00:00:15,390
+Microbiology العملي في هذا اللقاء هتكون المحاضرة
+
+4
+00:00:15,390 --> 00:00:19,810
+بعنوان Single Media Multiple Test في المعامل
+
+5
+00:00:19,810 --> 00:00:24,990
+السابقة كنا نستخدم ميديا بتكشف على فحص كيميائي
+
+6
+00:00:24,990 --> 00:00:29,680
+واحد one biochemical testاليوم هنتعامل مع ميديا
+
+7
+00:00:29,680 --> 00:00:34,240
+هتكشف على أكتر من biochemical test بقدر أعمل فيها
+
+8
+00:00:34,240 --> 00:00:39,060
+أكتر من فحص كيميائي من الأمثلة على high end media
+
+9
+00:00:39,060 --> 00:00:43,980
+في عندي أكتر من ميديا بقدر من خلالها أكشف على أكتر
+
+10
+00:00:43,980 --> 00:00:49,440
+من biochemical test SIM media trigger iron agar
+
+11
+00:00:49,860 --> 00:00:54,300
+Triple Sugar Iron Agar Lysine Iron Agar Motility
+
+12
+00:00:54,300 --> 00:00:59,360
+Indole Ornithine Media يبقى هاي الميديا من الأمثلة
+
+13
+00:00:59,360 --> 00:01:04,720
+على Single Media Multiple Test اسم الميديا جاي من
+
+14
+00:01:04,720 --> 00:01:08,360
+الفحوصات اللي أنا بقدر أعملها في هاي الميديا
+
+15
+00:01:08,360 --> 00:01:13,160
+هنتعرف في هذا المعمل على كل ميديا من هاي الميديات
+
+16
+00:01:13,160 --> 00:01:17,630
+بالتفصيلشو الفحوصات اللى بتكشف عنها كل ميديا كيف
+
+17
+00:01:17,630 --> 00:01:23,930
+أنا بقدر أفسر نتائج هذه الميديا خلينا نبدأ بأول
+
+18
+00:01:23,930 --> 00:01:28,690
+ميديا اللى هى الـ SIM media قولنا إن اسم الميديا
+
+19
+00:01:28,690 --> 00:01:32,850
+جاى من أسماء الفحوصات اللى أنا بقدر أعملها في هذه
+
+20
+00:01:32,850 --> 00:01:37,690
+الميديا بالنسبة لـ SIM media الـ S يرمز لـ
+
+21
+00:01:37,690 --> 00:01:44,340
+sulphideالـ I يرمز لـ Endol ال M يرمز لـ Motility
+
+22
+00:01:44,340 --> 00:01:48,480
+يبقى بقدر أكشف على ال sulphide على ال endol و على
+
+23
+00:01:48,480 --> 00:01:52,260
+ال motility تعرفنا في المعامل السابقة كيف أنا بدي
+
+24
+00:01:52,260 --> 00:01:56,320
+أقدر أكشف على ال motility و على ال endol لكن ما
+
+25
+00:01:56,320 --> 00:01:59,560
+تعرفنا على ال sulphide اليوم ان شاء الله هنتكلم
+
+26
+00:01:59,560 --> 00:02:05,000
+كيف أنا بدي أكشف على ال H2S production test عن أي
+
+27
+00:02:05,000 --> 00:02:09,820
+أنزين بالظبط بيكشف هذا الفحص شو نواتج الفحصوشو
+
+28
+00:02:09,820 --> 00:02:13,720
+بيعطيه نيلون الاندول والموتيليت اتعرفنا عليه هنمر
+
+29
+00:02:13,720 --> 00:02:17,520
+عليه على السريع بالنسبة لأول فحص اللي هو ال
+
+30
+00:02:17,520 --> 00:02:21,640
+sulfide ال sulfide باكشف على ال H2S production
+
+31
+00:02:21,640 --> 00:02:27,480
+test بدي اكشف على انزايم Sodium Thiosulfate
+
+32
+00:02:27,480 --> 00:02:31,980
+Reductase يبقى من اسمه Sodium Thiosulfate
+
+33
+00:02:31,980 --> 00:02:37,100
+Reductase هيعمل reduction لل Sodium Thiosulfate
+
+34
+00:02:37,510 --> 00:02:40,350
+يبقى ال substrate اللي لازم تكون موجودة في ال
+
+35
+00:02:40,350 --> 00:02:43,750
+media هي Sodium Thiosulfate يبقى هاي ال substrate
+
+36
+00:02:43,750 --> 00:02:48,010
+اللي هيشتغل عليها انزيم Sodium Thiosulfate
+
+37
+00:02:48,010 --> 00:02:54,310
+Reductase هيعمل لها reduction ناتج عملية الاختزال
+
+38
+00:02:54,310 --> 00:02:59,590
+هو ال sulphide بنهاية هيتكون hydrogen sulphide gas
+
+39
+00:02:59,590 --> 00:03:06,090
+اللي هو ال H2S gas هيتكون عندي H2S gasكيف بدي أكشف
+
+40
+00:03:06,090 --> 00:03:12,270
+على الـ H2S Gas؟ قبل إحنا كنا نقول في ال
+
+41
+00:03:12,270 --> 00:03:17,110
+fermentation كان نواتج العملية Gas كنا نضيف نحضر
+
+42
+00:03:17,110 --> 00:03:20,210
+هام إت يوب إذا أتكون air bubble بعرف إنه والله
+
+43
+00:03:20,210 --> 00:03:24,010
+أتكون عندي Gas لكن في هذه ال media هي ال media
+
+44
+00:03:24,010 --> 00:03:30,270
+تعتبر اللي هي فيها نسبة أجاري هي semi-solid بما أن
+
+45
+00:03:30,270 --> 00:03:33,330
+في عندي فحص motility بقى هتكون semi-solid media
+
+46
+00:03:34,210 --> 00:03:37,530
+الفحص ال fermentation كانت ال media broth فبالتالي
+
+47
+00:03:37,530 --> 00:03:41,730
+سهل أني أنا أحط درهام tube لكن في هذا الفحص ما
+
+48
+00:03:41,730 --> 00:03:46,290
+بقدر أحط درهام tube لأنه ال media متصلبة فيها نسبة
+
+49
+00:03:46,290 --> 00:03:49,670
+أجار فبالتالي صعب أني أنا أحط درهام tube فماقدر
+
+50
+00:03:49,670 --> 00:03:54,670
+أكشف على الجاز باستخدام الدرهام tubeكيف بدي اكشف
+
+51
+00:03:54,670 --> 00:04:00,530
+على ال gas ال H2S gas بقوللي بكشف على ال H2S gas
+
+52
+00:04:00,530 --> 00:04:04,910
+بإضافة indicator هذا ال indicator هيرتبط بال H2S
+
+53
+00:04:04,910 --> 00:04:10,390
+gas ويعطيني لون أسود يبقى لو البكتيريا عندها انزيم
+
+54
+00:04:10,740 --> 00:04:15,020
+Sodium Thiosulfate Reductase هتعمل اختزال للـ
+
+55
+00:04:15,020 --> 00:04:17,740
+Substrate اللي هي Sodium Thiosulfate الموجودة في
+
+56
+00:04:17,740 --> 00:04:23,260
+الميديا هينتج H2S Gas كيف بدي أخشف عنه الـ H2S Gas
+
+57
+00:04:23,260 --> 00:04:26,840
+هيربط ب Indicator هذا ال Indicator موجود في
+
+58
+00:04:26,840 --> 00:04:30,640
+الميديا اسمه Ferrous Ammonium Sulfate يبقى هذا هو
+
+59
+00:04:30,640 --> 00:04:35,080
+الـ H2S Indicator هيربط مع الـ H2S Gas لو
+
+60
+00:04:35,080 --> 00:04:38,460
+البكتيريا عندها أنزيم Sodium Thiosulfate Reductase
+
+61
+00:04:38,460 --> 00:04:43,920
+وتكون الـ H2S Gasهيتكون فررس سالفايد هذا المركب
+
+62
+00:04:43,920 --> 00:04:50,160
+black precipitate لونه أسود هيتكون هذا الراسب يبقى
+
+63
+00:04:50,160 --> 00:04:54,060
+لو اتكون عندي لون أسود معناه ان البكتيريا عندها
+
+64
+00:04:54,060 --> 00:04:58,620
+الانزيم كونت غاز اتفاعلت مع اتفاعل الجاز مع ال
+
+65
+00:04:58,620 --> 00:05:01,520
+indicator اللي هو الفررس أمانيوم سالفات ال H2S
+
+66
+00:05:01,520 --> 00:05:05,080
+indicator اللي موجود في ال media و أعطاني اللون
+
+67
+00:05:05,080 --> 00:05:11,250
+الأسودطيب بالنسبة لفحص الـ H2S production قولنا
+
+68
+00:05:11,250 --> 00:05:15,610
+إنه إذا اتكون اللون الأسود يبقى هي هذه positive
+
+69
+00:05:15,610 --> 00:05:19,550
+بتكون فيها انزيم الصادم ثيو سلفات Reductase اعرفنا
+
+70
+00:05:19,550 --> 00:05:24,590
+السبسترات اعرفنا ال enzyme اعرفنا من ال indicator
+
+71
+00:05:24,590 --> 00:05:29,820
+اللي موجود في ال medium طيب كأي فحص كيميائيأكيد
+
+72
+00:05:29,820 --> 00:05:34,200
+البكتيريا بتعمل أو عندها هذا الانزيم بيكون لهدف
+
+73
+00:05:34,200 --> 00:05:38,460
+بيكون فيه فائدة من وجود هذا الانزيم في ال in vivo
+
+74
+00:05:38,460 --> 00:05:41,700
+انا بتكلم عن ان in vivo زي ما قولنا مثلا في فحص
+
+75
+00:05:41,700 --> 00:05:45,960
+الكتلاز كانت تتخلص من ال hydrogen peroxide ال
+
+76
+00:05:45,960 --> 00:05:50,480
+toxic هنا الفائدة من ان البكتيريا تعمل reduction
+
+77
+00:05:50,480 --> 00:05:55,780
+للصلفاتإنه احنا قلنا من ضمن ال inorganic molecule
+
+78
+00:05:55,780 --> 00:05:59,980
+اللي بتستعملها البكتيريا كمستقبل نهائي للإلكترونات
+
+79
+00:05:59,980 --> 00:06:04,120
+في الآن aerobic respiration عشان تنتج الطاقة
+
+80
+00:06:04,120 --> 00:06:09,280
+السلفر يبقى هذا من فوائد هذا الانزيم انه بتعمل
+
+81
+00:06:09,280 --> 00:06:13,310
+reduction للسلفات عشانأنا أستخدم الـ Sulphur
+
+82
+00:06:13,310 --> 00:06:16,150
+كمستقبل نهائي للإلكترونات في الان Europe
+
+83
+00:06:16,150 --> 00:06:20,190
+Prespiration لإنتاج الطاقة طيب خلصنا من أول فحص
+
+84
+00:06:20,190 --> 00:06:25,830
+اللي هو ال H2S Production Testنجي للفحص التاني
+
+85
+00:06:25,830 --> 00:06:30,530
+الاندول طبعا الاندول مر معنا في ال chapter اللي
+
+86
+00:06:30,530 --> 00:06:35,050
+قبل قولنا الاندول بيكشف على أنزيم لتريبتوفاناز
+
+87
+00:06:35,050 --> 00:06:38,970
+لتريبتوفاناز من اسمه هيشتغل على ال substrate
+
+88
+00:06:38,970 --> 00:06:42,770
+لتريبتوفان اللي هي ال amino acid هذي لتريبتوفان
+
+89
+00:06:42,770 --> 00:06:47,450
+بتتكون من اندول وبيوروفات وNH3 مرتبطين ببعض لو
+
+90
+00:06:47,450 --> 00:06:51,770
+أنزيم لتريبتوفاناز موجود في البكتيريا هيكسر
+
+91
+00:06:51,770 --> 00:06:58,590
+لتريبتوفان لاندولpyruvate and NH3 هضيف ال covax
+
+92
+00:06:58,590 --> 00:07:01,950
+reagent بنضيف ال covax reagent هو هذا ال reagent
+
+93
+00:07:01,950 --> 00:07:07,350
+بنضيفه بعد ال incubation بنضيفه على ال media هذا
+
+94
+00:07:07,350 --> 00:07:11,710
+ال covax reagent بيرتبط مع ال indol ناتج من نواتج
+
+95
+00:07:11,710 --> 00:07:16,690
+عملية تكسير للتريبتوفان هيرتبط فيه ويتكون red ring
+
+96
+00:07:16,690 --> 00:07:20,010
+قولنا هاي ال red ring بيكون لونها لون الكرز ال
+
+97
+00:07:20,010 --> 00:07:23,950
+cherry red ringلو اتكونت يبقى هي indol positive
+
+98
+00:07:23,950 --> 00:07:28,010
+بالنسبة لفحص ال motility اتكلمنا انا عشان اشتغل
+
+99
+00:07:28,010 --> 00:07:32,190
+فحص ال motility بزرع البكتيريا في ميديا semi solid
+
+100
+00:07:32,190 --> 00:07:37,170
+media هي الميديا فيها نسبة الأجار 4 من 10% أجار
+
+101
+00:07:37,170 --> 00:07:43,190
+الفكرة انه لو البكتيريا متع المتحركة هلاحظ انه
+
+102
+00:07:43,190 --> 00:07:48,550
+النمو هيكونعلى خط الزراعه وهينتشر بعيدا عن خط
+
+103
+00:07:48,550 --> 00:07:52,350
+الزراعه لكن لو كانت non motile فقط على خط الزراعه
+
+104
+00:07:52,350 --> 00:07:56,790
+هلاقي النمو هذا بالنسبة لفحص ال motility طبعا انا
+
+105
+00:07:56,790 --> 00:08:01,450
+هعمل stabbing يبقى ميديا ال SIM اي ميديا فيها فحص
+
+106
+00:08:01,450 --> 00:08:05,650
+ال motility هتكون الزراعه stabbing هتكون stab tube
+
+107
+00:08:05,650 --> 00:08:08,770
+هزرع stabbing هاخد البكتيريا اللي بدي اكشف عنها
+
+108
+00:08:08,770 --> 00:08:13,830
+هعمل stabbing ل ال media و هعمل incubation لمدة 4
+
+109
+00:08:14,320 --> 00:08:20,760
+بعشرين ساعة هلأ فيها ملاحظتين في هي ال media
+
+110
+00:08:20,760 --> 00:08:25,480
+المحلاحظة الأولى ان انا بعد اربع عشرين ساعة بقرأ
+
+111
+00:08:25,480 --> 00:08:31,880
+نتائج اللي هو فحوصات هي ال media بقولي بقرأ فحص ال
+
+112
+00:08:31,880 --> 00:08:37,300
+motility بعدين بقرأ فحص ال sulfide بعدين بقرأ فحص
+
+113
+00:08:37,300 --> 00:08:42,610
+ال indoor من خلال إضافة ال covax reagent يبقىلازم
+
+114
+00:08:42,610 --> 00:08:48,450
+او ممنوع اقرأ فحص الاندول الا بعد قراءة فحص ال
+
+115
+00:08:48,450 --> 00:08:52,170
+motility why هى يوهان مكتوب بالترتيب بقرأ فحص ال
+
+116
+00:08:52,170 --> 00:08:56,030
+motility بعدين ال H2O production بعدين بضيف ال
+
+117
+00:08:56,030 --> 00:09:00,070
+covax reagent عشان اقرأ ال endol test هيك بالترتيب
+
+118
+00:09:00,070 --> 00:09:03,910
+ممنوع اضيف ال covax reagent و اقرأ ال endol test
+
+119
+00:09:03,910 --> 00:09:09,450
+بعدين اقرأ فحص ال motility هذا سؤال مهم السبب انه
+
+120
+00:09:09,940 --> 00:09:15,040
+الـ COVAX REAGENT قلنا أحد مكوناته الـ HCL ذكرنا
+
+121
+00:09:15,040 --> 00:09:19,260
+سابقاً لما شرحنا فحص الـ Endowel Test بالتفصيل أحد
+
+122
+00:09:19,260 --> 00:09:24,440
+مكونات الـ COVAX REAGENT هو الـ HCL بقول الـ HCL
+
+123
+00:09:24,440 --> 00:09:28,900
+بقلل الـ turbidity الموجودة في الميديا و بيخليها
+
+124
+00:09:28,900 --> 00:09:34,020
+clear بعمل clearance للميدياطيب فحص ال motility
+
+125
+00:09:34,020 --> 00:09:39,000
+بعتمد على ال turbidity انه لو البكتيريا كانت على
+
+126
+00:09:39,000 --> 00:09:42,060
+خط الزراعه نمت على خط الزراعه واتجهت بعيدا عن خط
+
+127
+00:09:42,060 --> 00:09:44,700
+الزراعه بيكون فيها انديه turbidity بقرأ ال
+
+128
+00:09:44,700 --> 00:09:47,600
+turbidity فبعرف ان هي motile لكن لو كانت فقط على
+
+129
+00:09:47,600 --> 00:09:52,220
+خط الزراعه يبقى non motile يبقى قراءة فحص ال
+
+130
+00:09:52,220 --> 00:09:56,020
+motility بعتمد على ال turbidity طب ال Kovacs
+
+131
+00:09:56,020 --> 00:09:59,280
+reagent قلنا ان هو بقلل ال turbidity بعمل
+
+132
+00:09:59,280 --> 00:10:03,790
+clearance ل الميديافبالتالي مش هعرف أقرأ فحص الـ
+
+133
+00:10:03,790 --> 00:10:09,410
+Motility صح لأنه بيعتمد على الـ turbidity عشان هيك
+
+134
+00:10:09,410 --> 00:10:13,690
+بقرأ فحص الـ Motility بشوف هل هي على خط الزراعة
+
+135
+00:10:13,690 --> 00:10:18,490
+فقط يبقى non-motile هل هي طلعت عن خط الزراعة اتجهت
+
+136
+00:10:18,490 --> 00:10:23,350
+بعيدا عن خط الزراعة يبقى هي motile و بعدين بسجل
+
+137
+00:10:23,350 --> 00:10:27,210
+النتيجة و بعدين بضيف ال Covax reagent عشان أقرأ ال
+
+138
+00:10:27,210 --> 00:10:32,950
+Endol testهذا بالنسبة لأول ملاحظة الملاحظة التانية
+
+139
+00:10:32,950 --> 00:10:38,250
+أنه لو البكتيريا H2S positive لو كان عندها انزيم
+
+140
+00:10:38,250 --> 00:10:43,770
+الصيوم ثيوكسلفاتريدكتاز وأنتجت الـ H2S gas ارتبط
+
+141
+00:10:43,770 --> 00:10:47,090
+مع الـ H2S indicator و أعطاني فرر السلفايد اللي
+
+142
+00:10:47,090 --> 00:10:51,950
+يعتبر black precipitate بتكون عندى لون أسود غامق
+
+143
+00:10:51,950 --> 00:10:57,360
+في كل ال mediaطب هل هعرف نتيجة الـ motility لو
+
+144
+00:10:57,360 --> 00:11:00,940
+اتكون لون أسود في ال media أكيد لأ مش هعرف نتيجة
+
+145
+00:11:00,940 --> 00:11:05,220
+ال motility بالتالي لو البكتيريا كانت H2S
+
+146
+00:11:05,220 --> 00:11:10,020
+production positive ال motility ماقدر أتعرف عليها
+
+147
+00:11:10,020 --> 00:11:15,520
+بكتب في نتيجة unknown يبقى لو إجاني سؤالصورة
+
+148
+00:11:15,520 --> 00:11:20,460
+موجودة عليها H2S كان لونها أسود H2S positive فحص
+
+149
+00:11:20,460 --> 00:11:23,800
+الـ motility بنكتب unknown ما بقدر أتعرف على فحص
+
+150
+00:11:23,800 --> 00:11:28,180
+ال motility لأن اللون الأسود غطى على ال media غطى
+
+151
+00:11:28,180 --> 00:11:31,920
+على فحص ال motility وعلى ال turbidity طب إيش الحل
+
+152
+00:11:31,920 --> 00:11:35,940
+عشان أعرف هل البكتيريا motile أو non motile بقولنا
+
+153
+00:11:35,940 --> 00:11:40,760
+بنحضر media خاصة semi solid media فيها نسبة الأجار
+
+154
+00:11:40,760 --> 00:11:46,690
+4 من 10%بدون ما أضيف اللي هو الـ Sodium
+
+155
+00:11:46,690 --> 00:11:50,490
+Thiosulfate الـ Substrate والـ H2S Indicator بدون
+
+156
+00:11:50,490 --> 00:11:54,990
+ما أضيف أي Substrate أو أي Indicator لأي فحوصات
+
+157
+00:11:54,990 --> 00:11:59,410
+تانية زي ما اشتغلنا في أول فحص في الـ Motility
+
+158
+00:11:59,410 --> 00:12:03,510
+كانت هي فقط high and medium nutrient broth مع نسبة
+
+159
+00:12:03,510 --> 00:12:07,990
+أجار 4 من 10%مافيها أي substrate لأي فحوصات
+
+160
+00:12:07,990 --> 00:12:11,810
+كيميائية تانية فهي فقط هتكشف على ال motility هذا
+
+161
+00:12:11,810 --> 00:12:16,250
+بالنسبة للحلقة فكما نكون خلصنا تقريبا أو أجملنا ال
+
+162
+00:12:16,250 --> 00:12:20,430
+SIM media عن شو الفحوصات اللي بتكشف عنها شو
+
+163
+00:12:20,430 --> 00:12:24,150
+النتيجة ال positive و ال negative لكل فحص كيف أنا
+
+164
+00:12:24,150 --> 00:12:29,410
+بدي أعمل هذا الفحص و كيف بدي أقرأ النتائج نيجي لل
+
+165
+00:12:29,410 --> 00:12:34,690
+slidesهي ال media قولنا semi-solid media SIM test
+
+166
+00:12:34,690 --> 00:12:37,910
+medium is a semi-solid media يعني نسبة الأجار فيها
+
+167
+00:12:37,910 --> 00:12:42,590
+4 من 10% قولنا أي ميديا فيها فحص ال motility فهي
+
+168
+00:12:42,590 --> 00:12:47,120
+semi-solid mediaUsed as a differential test medium
+
+169
+00:12:47,120 --> 00:12:51,360
+أكيد بما أنه بتعطيني أو بتفرقلي بكتيريا عن بكتيريا
+
+170
+00:12:51,360 --> 00:12:55,900
+يبقى هي differential test media في أعطت مثلا بعض
+
+171
+00:12:55,900 --> 00:12:58,840
+البكتيريا اللي عندها القدرة على إنتاج الـH2S gas
+
+172
+00:12:58,840 --> 00:13:01,980
+لون أسود لكن البكتيريا اللي ما عندها القدرة إنها
+
+173
+00:13:01,980 --> 00:13:05,200
+تنتج الـH2S ما كان فيه لون أسود يبقى هيعتبرها
+
+174
+00:13:05,200 --> 00:13:08,850
+differential test mediumقلنا الاس بورزموس
+
+175
+00:13:08,850 --> 00:13:13,770
+لاستلفايد من خلال فحص الـH2O production بكشف عن
+
+176
+00:13:13,770 --> 00:13:18,010
+قدرة البكتيريا إنها تستهلك الـ Sodium Thiosulfate
+
+177
+00:13:18,010 --> 00:13:23,130
+قلنا هذا الـsubstrate اللي هيشتغل عليه الإنزيمthey
+
+178
+00:13:23,130 --> 00:13:26,110
+can reduce it to sulfide using the enzyme
+
+179
+00:13:26,110 --> 00:13:31,630
+thiosulfate reductase قولنا من خلال انزيم ال
+
+180
+00:13:31,630 --> 00:13:34,210
+thiosulfate reductase أو ال sodium thiosulfate
+
+181
+00:13:34,210 --> 00:13:38,710
+reductase اللي موجود في بعض أنواع البكتيريا هتعمله
+
+182
+00:13:38,710 --> 00:13:41,850
+البكتيريا reduction لل sodium thiosulfate
+
+183
+00:13:41,850 --> 00:13:45,110
+السبسترات الموجود في الميديا هينتج بالنهاية
+
+184
+00:13:45,110 --> 00:13:49,770
+sulfite او هينتج بالنهاية هينتج sulfite هتعمله
+
+185
+00:13:49,770 --> 00:13:53,560
+reduction و بالنهاية هيتكونالـ hydrogen sulphide
+
+186
+00:13:53,560 --> 00:13:59,000
+gas بالنسبة للـ I الـ I قلنا هي بتكشف عن الاندول
+
+187
+00:13:59,000 --> 00:14:04,280
+باكشف عن قدرة البكتيريا انها تنتج لـ tryptophanase
+
+188
+00:14:04,280 --> 00:14:07,620
+قلنا أنزيم لـ tryptophanase بيكسر ال amino acid
+
+189
+00:14:07,620 --> 00:14:11,840
+الـ tryptophan لاندول امانيام and pyruvic acid ال
+
+190
+00:14:11,840 --> 00:14:15,140
+motility بدي اكشف على ال motility ال motility هو
+
+191
+00:14:15,140 --> 00:14:20,570
+وجود الفلاجلةهل البكتيريا بتقدر تسبح خلال ال media
+
+192
+00:14:20,570 --> 00:14:25,450
+هل بتقدر تتجه بعيدا عن خط الزراعة ولا لأ مكونات ال
+
+193
+00:14:25,450 --> 00:14:30,250
+media نسبة الأجار 4 من 10% أجار هذا لفحص مهم جدا
+
+194
+00:14:30,250 --> 00:14:33,830
+لفحص ال mortality عشان هيك ال media تعتبر semi
+
+195
+00:14:33,830 --> 00:14:37,850
+solid media بيبتون البيبتون هو عبارة عن قلنا
+
+196
+00:14:37,850 --> 00:14:41,700
+مجموعة من ال amino acid مرتبطة مع بعضها البعضwhich
+
+197
+00:14:41,700 --> 00:14:45,180
+is rich in tryptophan amino acid يبقى هو موجود فيه
+
+198
+00:14:45,180 --> 00:14:48,880
+هو ال tryptophan اللي يعتبر substrate مهم لفحص
+
+199
+00:14:48,880 --> 00:14:52,900
+الإندول الصواتيومثيو سلفات قلنا substrate لفحص ال
+
+200
+00:14:52,900 --> 00:14:57,360
+H2S production يبقى هدول التلاتة substrate لكل فحص
+
+201
+00:14:57,360 --> 00:15:01,960
+من الفحوصات التلاتة اللي بتكشف عنهم ميديا ال SIM
+
+202
+00:15:01,960 --> 00:15:06,160
+في الراس أمانيوم سلفات قلنا هو ال H2S indicator
+
+203
+00:15:06,160 --> 00:15:11,520
+اللي هيكشف على وجود ال H2S gasهذه الميديا SIM
+
+204
+00:15:11,520 --> 00:15:16,720
+بتكشف على تلت أنشطة بتقوم بيها الـ Enteric
+
+205
+00:15:16,720 --> 00:15:21,740
+Bacteria Enteric Bacteria بتفرق لي بعنهم أول إيش
+
+206
+00:15:21,740 --> 00:15:24,620
+قلنا اللي هو فحص السلفات Sodium Thiosulfate
+
+207
+00:15:24,620 --> 00:15:27,240
+السبسترات اللي هيشتغل عليها أنزيم Sodium
+
+208
+00:15:27,240 --> 00:15:30,460
+Thiosulfate Reductase First Ammonium Sulfate هو
+
+209
+00:15:30,460 --> 00:15:34,540
+الـH2S Indicatorعشان أكشف على الـ hydrogen sulfide
+
+210
+00:15:34,540 --> 00:15:38,000
+production اللي هو الـ H2S production قلنا
+
+211
+00:15:38,000 --> 00:15:40,240
+الferrous ammonium sulfate اللي هو الـ H2S
+
+212
+00:15:40,240 --> 00:15:44,200
+indicator بيرتبط مع الـ H2S gas اللي ينتج من عملية
+
+213
+00:15:44,200 --> 00:15:48,100
+ال reduction عشان ينتج بالنهاية الferrous sulfide
+
+214
+00:15:48,100 --> 00:15:53,290
+هذا المركب لونه أسود black precipitateتانى نشاط
+
+215
+00:15:53,290 --> 00:15:57,490
+بتكشف عنه هذه ال media اللى هو الفحص الاندول
+
+216
+00:15:57,490 --> 00:16:03,070
+البيبتون موجود is rich in tryptophan بقولنا انه
+
+217
+00:16:03,070 --> 00:16:07,990
+البيبتون بيحتوي على ال tryptophan بدي اكشف هل
+
+218
+00:16:07,990 --> 00:16:12,590
+البكتيريا بتقدر انها تهاجم هذا ال tryptophan تكسر
+
+219
+00:16:12,590 --> 00:16:16,730
+ال tryptophan ناتجة عملية التكسير هيتكون عندى
+
+220
+00:16:16,730 --> 00:16:20,930
+اندول كيف بكشف على الاندول بضيف ال covax reagent
+
+221
+00:16:21,790 --> 00:16:25,710
+فحص الـ motility فحص الـ motility أكيد بقدر أني
+
+222
+00:16:25,710 --> 00:16:29,150
+أنا أكشف عنه في هذه ال media نتيجة الـ semi-solid
+
+223
+00:16:29,150 --> 00:16:32,030
+nature of the media نتيجة أنه ال media هي semi
+
+224
+00:16:32,030 --> 00:16:35,310
+-solid فبالتالي لو البكتيريا عندها القدرة أنها
+
+225
+00:16:35,310 --> 00:16:39,310
+تنمو radiating out from the center step line لو
+
+226
+00:16:39,310 --> 00:16:43,190
+قدر تنمو بعيدا عن خط الزراعي يبقى هذا يشير أنه
+
+227
+00:16:43,190 --> 00:16:48,320
+البكتيريا أو test organism is motileبالنسبة لـ
+
+228
+00:16:48,320 --> 00:16:53,340
+Significant لهذا الفحص قلنا أي فحص بيكون في يد انا
+
+229
+00:16:53,340 --> 00:16:56,500
+بيكون لإله Significant أهمية عشان يفرق بين مين
+
+230
+00:16:56,500 --> 00:17:00,000
+ومين فحص الـ Motility اتكلمنا عنه لما شرحنا على
+
+231
+00:17:00,000 --> 00:17:02,820
+البكتيريا الـ Motility و الـ Indoor لما شرحناهم
+
+232
+00:17:02,820 --> 00:17:07,880
+بالتفصيللكن بالنسبة للـ H2S production الـ H2S
+
+233
+00:17:07,880 --> 00:17:12,060
+production بقول لي إنه الـ significant لهذا الفحص
+
+234
+00:17:12,060 --> 00:17:15,940
+the ability to reduce sulfur is specific to two
+
+235
+00:17:15,940 --> 00:17:19,140
+members of the enteric family Salmonella and
+
+236
+00:17:19,140 --> 00:17:24,680
+Brutias بقول لي إن قدرة البكتيريا إنها تعمل اختصار
+
+237
+00:17:24,680 --> 00:17:30,660
+للسلفر فقط بكتيريتين تنتين هم اللي H2S production
+
+238
+00:17:30,660 --> 00:17:34,140
+positiveمن الـ Enteric family اللي هي عائلة الـ
+
+239
+00:17:34,140 --> 00:17:38,520
+Enterobacteriaceae السلمونيلا و البروتياس التانتان
+
+240
+00:17:38,520 --> 00:17:43,940
+هدول H2S positive احنا قلنا اذا بتتذكروا بفحص
+
+241
+00:17:43,940 --> 00:17:49,520
+البروتياس بفحص اليورياز sorry بفحص اليورياز قلنا
+
+242
+00:17:49,520 --> 00:17:54,400
+بيفرق بين ال non-lactose fermenter اللي هم
+
+243
+00:17:54,400 --> 00:17:58,600
+السلمونيلا شجلة البروتياس هدولة التلاتة يعتبروا
+
+244
+00:17:58,600 --> 00:18:02,930
+non-lactose fermenterموجودين في عينات ال stone
+
+245
+00:18:02,930 --> 00:18:07,370
+قلنا عشان افرق بينهم بعمل فحص اليورياز لو طلعت
+
+246
+00:18:07,370 --> 00:18:11,670
+يورياز positive يبقى بروتياس لو طلعت يورياز
+
+247
+00:18:11,670 --> 00:18:16,690
+negative يبقى سلمونيلا او شجلة طيب السلمونيلا و
+
+248
+00:18:16,690 --> 00:18:21,590
+الشجلة كيف بدي افرق بينهم؟بقول من خلال فحص ال
+
+249
+00:18:21,590 --> 00:18:27,450
+motility و ال H2S production السلمونيلا H2S
+
+250
+00:18:27,450 --> 00:18:32,390
+production positive و motile لكن شجلة non motile و
+
+251
+00:18:32,390 --> 00:18:38,470
+non H2S او تعتبر H2S production negative البروتياس
+
+252
+00:18:38,470 --> 00:18:43,310
+نفس السلمونيلا motile زاد H2S positive لكن كيف
+
+253
+00:18:43,310 --> 00:18:46,830
+بفرق بينهم من خلال فحص اليوريايبقى هي مكتوب
+
+254
+00:18:46,830 --> 00:18:51,650
+السلمونيلا H2S positive and motile لبروتياز H2S
+
+255
+00:18:51,650 --> 00:18:54,810
+positive and motile كيف بفرق بينهم من خلال اتكلمنا
+
+256
+00:18:54,810 --> 00:18:57,950
+عنه هذا الاشي سابقا من خلال فحص اليورياز
+
+257
+00:18:57,950 --> 00:19:03,370
+السلمونيلا يورياز negative لبروتياز positive
+
+258
+00:19:03,370 --> 00:19:10,470
+الشجلة عكس السلمونيلا و لبروتياز H2S negative and
+
+259
+00:19:10,470 --> 00:19:15,280
+non motile لكن هي يورياز negativeالـ Eclipsella
+
+260
+00:19:15,280 --> 00:19:20,480
+اتفقنا إنه هي فقط الوحيدة الـ non-motile من
+
+261
+00:19:20,480 --> 00:19:25,900
+الكوليفارم and doesn't produce H2S الـ E.coli
+
+262
+00:19:25,900 --> 00:19:31,320
+تعتبر motile وكمان doesn't produce H2S قلنا في
+
+263
+00:19:31,320 --> 00:19:35,080
+عندي فحص اللي هو ال MVEC بقدر أفرق بين ال E.coli و
+
+264
+00:19:35,080 --> 00:19:39,360
+ال E.clipsella ال MVEC اللي هي بفرق بين الكوليفارم
+
+265
+00:19:39,360 --> 00:19:45,530
+حتى ككل مش بس ال E.clipsella و E.coliكيف أنا بدي
+
+266
+00:19:45,530 --> 00:19:49,050
+أشغل هذا الفحص أكيد بعمل label للي تيوب الـ SIM
+
+267
+00:19:49,050 --> 00:19:53,810
+أجار دي بتيوب كيف بتكون هي أجار من أشكال من أشكال
+
+268
+00:19:53,810 --> 00:19:58,510
+اللي هو ال media في ال SIM بتكون أجار دي بتيوب
+
+269
+00:19:58,510 --> 00:20:03,270
+بعمل label باسم البكتيريا اللي بدي أكشف عنها و
+
+270
+00:20:03,270 --> 00:20:09,790
+بكتب طبعا اللي هو تاريخ الزراعةبعمل طبعا ال
+
+271
+00:20:09,790 --> 00:20:12,770
+stabbing بستخدم ال aseptic technique بمنع أي كنت
+
+272
+00:20:12,770 --> 00:20:17,950
+ماناشي انه سير بعمل ال stabbing ل ال media بحاول
+
+273
+00:20:17,950 --> 00:20:21,310
+ان انا ابعد على ال bottom of the tube اخر ال tube
+
+274
+00:20:21,310 --> 00:20:27,610
+ما بعمل ال stabbing له تقريبا ببعد شوية مسافةبعمل
+
+275
+00:20:27,610 --> 00:20:32,950
+incubation لمدة 24 ساعة درجة حرارة 37 هنكتب
+
+276
+00:20:32,950 --> 00:20:36,230
+لملاحظة قولناها سابقا ان لو البكتيري عندها القدرة
+
+277
+00:20:36,230 --> 00:20:40,190
+انها تنتج ال hydrogen sulfide يبقى بالنهاية هيتكون
+
+278
+00:20:40,190 --> 00:20:43,990
+black precipitate فبالتالي مش هقدر اكشف على ال
+
+279
+00:20:43,990 --> 00:20:48,410
+motility of organismعشان هيك بفكر أن فحص ال
+
+280
+00:20:48,410 --> 00:20:53,150
+motility هو false negative result مش هيبين عندي مش
+
+281
+00:20:53,150 --> 00:20:56,970
+هتبين الحركة فبالتالي هفكرها negative لكن هي false
+
+282
+00:20:56,970 --> 00:21:02,190
+negative resultبالـ H2S Production اللون الأسود
+
+283
+00:21:02,190 --> 00:21:05,850
+اللي اتكون هو اللي خلاني ما أشوف الـ Turbidity
+
+284
+00:21:05,850 --> 00:21:10,870
+فبالتالي أحدد الـ Motility للبكتيريا عشان هيك الحل
+
+285
+00:21:10,870 --> 00:21:15,970
+أني أنا بعمل Semi Solid Media بدون ما يكون فيه
+
+286
+00:21:15,970 --> 00:21:19,010
+اندي Substrate اللي هي الـ Sodium Thiosulfate أو
+
+287
+00:21:19,010 --> 00:21:22,810
+الـ H2S Indicator فقط بتتكون من الـ Nutrient
+
+288
+00:21:22,810 --> 00:21:33,130
+Growth مع نسبة أجار 4 من 10%بالنسبة للنتائج هي
+
+289
+00:21:33,130 --> 00:21:37,940
+الصورتان نفس الشيءأول شئ هنقرأ فحص الـ Motility
+
+290
+00:21:37,940 --> 00:21:42,120
+أكيد اللي بالنسبة لـ Motility هي الـ bacteria موطة
+
+291
+00:21:42,120 --> 00:21:46,080
+اللي بنلاحظ أنه في عندي بعيدا عن خط الزراعة في
+
+292
+00:21:46,080 --> 00:21:50,860
+عندي turbidity بعدها هقرأ الـ edge to us هي
+
+293
+00:21:50,860 --> 00:21:55,120
+negative edge to us negative فحص ال Indol دفت
+
+294
+00:21:55,120 --> 00:21:58,980
+الكوفاكس رياجانت ماتحول اللون للون أحمر في أعلى
+
+295
+00:21:58,980 --> 00:22:03,080
+الحلقة يبقى هي في أعلى ال tube يبقى هي Indol
+
+296
+00:22:03,080 --> 00:22:08,130
+negativeهان بالنسبة لفحص الـ Motility من اللحظة
+
+297
+00:22:08,130 --> 00:22:13,070
+أنه على خط الزراعي يبقى هي non-motile فحص الـ H2S
+
+298
+00:22:13,070 --> 00:22:17,150
+نيجاتيف فحص الاندول positive اتكوّن لون cherry red
+
+299
+00:22:17,150 --> 00:22:24,250
+يبقى هي positive هان H2S positive ماقدر اكشف على
+
+300
+00:22:24,250 --> 00:22:28,030
+ال motility فبكتب unknown ال motility بكتب عنها
+
+301
+00:22:28,030 --> 00:22:31,710
+unknown بالنسبة لالاندول negative
+
+302
+00:22:34,450 --> 00:22:39,650
+هذه الـ tube هي ك control مش مزروعة هذه ال tube
+
+303
+00:22:39,650 --> 00:22:43,890
+نقرأ أول إشي نتيجة ال motility فقط هي هنا على خط
+
+304
+00:22:43,890 --> 00:22:49,330
+الزراعية هي يبقى هي non motile بالنسبة ل ال H2S
+
+305
+00:22:49,330 --> 00:22:52,950
+negative بالنسبة ل ال indol من اللاحظ في عندي لون
+
+306
+00:22:52,950 --> 00:22:58,530
+أحمر يبقى هي indol positive هنا ال H2S positive
+
+307
+00:22:58,530 --> 00:23:02,780
+طبعا ال motility unknownبالنسبة للاندول positive
+
+308
+00:23:02,780 --> 00:23:06,880
+هان ال H2S positive بالنسبة للموتيليتي unknown
+
+309
+00:23:06,880 --> 00:23:10,500
+بالنسبة للاندول negative يبقى هيك نقرأ النتيجة
+
+310
+00:23:10,500 --> 00:23:16,380
+بنكتب التلت فحوصات SIM عشان ما نطلع خبطش نكتب S
+
+311
+00:23:16,380 --> 00:23:21,090
+اللي هو لسه الفايد I للاندول M للموتيليتيبنقرأ أول
+
+312
+00:23:21,090 --> 00:23:24,210
+issue ال motility بعدين ال S2S بعدين بنضيف ال
+
+313
+00:23:24,210 --> 00:23:27,910
+Govex reagent وبنقرأ نتيجة ال indoor هيك بنكون
+
+314
+00:23:27,910 --> 00:23:34,430
+خلصنا أول media اللي هي ال SIM media نيجي لتاني
+
+315
+00:23:34,430 --> 00:23:39,150
+media تعتبر single media multiple test اللي هي ل
+
+316
+00:23:39,150 --> 00:23:44,970
+triple sugar iron agar or clicar iron agarهدولا
+
+317
+00:23:44,970 --> 00:23:49,950
+الميديتين نفس ال media لكن الاختلاف انه ال kliger
+
+318
+00:23:49,950 --> 00:23:55,650
+iron agar بتحتوي على نوعين من أنواع السكر اللي هم
+
+319
+00:23:55,650 --> 00:24:00,410
+ال dextrose اللي هو الجليكوز زائد اللاكتوز لكن ال
+
+320
+00:24:00,410 --> 00:24:04,230
+triple sugar iron agar بتحتوي على ثلاث أنواع من
+
+321
+00:24:04,230 --> 00:24:09,500
+السكريات من اسمها tripledextrose زي الكليجر زائد
+
+322
+00:24:09,500 --> 00:24:13,080
+lactose برضه زي الكليجر لكن الإضافة المختلفة عن
+
+323
+00:24:13,080 --> 00:24:17,160
+الكليجر اللي هي الساكروز يبقى اتنتاين نفس الاشي
+
+324
+00:24:17,160 --> 00:24:22,880
+لكن الفرق انه سكر الساكروز مش موجود في الكليجر
+
+325
+00:24:22,880 --> 00:24:27,580
+iron agar فقط موجود في ال triple sugar iron agar
+
+326
+00:24:27,580 --> 00:24:33,690
+هي ال media من خلالها بكشف علىدي اميناشن للبيبتون
+
+327
+00:24:33,690 --> 00:24:38,430
+ال fermentation وال H2S production ففي هذه ال
+
+328
+00:24:38,430 --> 00:24:44,810
+media منصبها في slant slant هي بتكون slant اتيوب
+
+329
+00:24:44,810 --> 00:24:50,310
+ليش انا صبيتها في slant اتيوب عشان اوفر ظروف
+
+330
+00:24:50,310 --> 00:24:54,810
+هوائية aerobic condition من خلال ال slant سطح ال
+
+331
+00:24:54,810 --> 00:24:58,680
+slant هذا موفر aerobic conditionوهذا الـ
+
+332
+00:24:58,680 --> 00:25:01,580
+deamination اللي اللي بيبتوع بيحصل في ظروف aerobic
+
+333
+00:25:01,580 --> 00:25:07,060
+condition كمان بدي أوفر من خلال البط او اللي تحت
+
+334
+00:25:07,060 --> 00:25:10,140
+ال slant هذا بسميه بط اللي بكون موجود تحت هذا
+
+335
+00:25:10,140 --> 00:25:13,920
+بسميه بط هذا بوفر ظروف an aerobic condition ظروف
+
+336
+00:25:13,920 --> 00:25:17,620
+لا هوائية ال fermentation و القشط و أسي production
+
+337
+00:25:17,620 --> 00:25:23,030
+بيحصلوا في ظروف لا هوائية عشان هيك بديأنا أوفر
+
+338
+00:25:23,030 --> 00:25:27,710
+ظروف هوائية وظروف لا هوائية فبصب ال media ب slant
+
+339
+00:25:27,710 --> 00:25:30,730
+لأن ال-d-amination للبيبتون بيصير في ظروف European
+
+340
+00:25:30,730 --> 00:25:33,710
+condition ال fermentation و ال-H2S production
+
+341
+00:25:33,710 --> 00:25:38,410
+بيصير في ظروف Un-European condition يبقى ال slant
+
+342
+00:25:38,410 --> 00:25:41,930
+هكشف على ال-d-amination للبيبتون ال-but هكشف على
+
+343
+00:25:41,930 --> 00:25:45,990
+ال fermentation و ال-H2S production في high ال
+
+344
+00:25:45,990 --> 00:25:50,800
+media في عندي BH indicatorلش لأنه نواتج عملية ال
+
+345
+00:25:50,800 --> 00:25:54,280
+fermentation acid و نواتج عملية ال deamination
+
+346
+00:25:54,280 --> 00:25:57,400
+للبيبتون في عندي alkaline product فبالتالي بما أنه
+
+347
+00:25:57,400 --> 00:26:00,680
+اتكون alkaline او ال acidic product هضيف BH
+
+348
+00:26:00,680 --> 00:26:05,280
+Indicator عشان استدل على حدوث ال fermentation او
+
+349
+00:26:05,280 --> 00:26:09,940
+ال deamination للبيبتون ال BH Indicator الموجود في
+
+350
+00:26:09,940 --> 00:26:13,600
+ال media هو ال phenol red يعني ال phenol red مر
+
+351
+00:26:13,600 --> 00:26:17,400
+معنى للمرة التالتة اول مرة مر في فحص ال
+
+352
+00:26:17,400 --> 00:26:21,370
+fermentationتاني مرة في فحص اليوريا تالت مرة في
+
+353
+00:26:21,370 --> 00:26:25,530
+فحص يوليس ريبيل شوجر ايرون اجار حدوده في ال acid
+
+354
+00:26:25,530 --> 00:26:28,990
+بيكون لونه yellow في ال alkaline بيكون لونه red في
+
+355
+00:26:28,990 --> 00:26:32,990
+ال neutral بيكون لونه orange بما اني انا بدي اكشف
+
+356
+00:26:32,990 --> 00:26:37,150
+على H2S production يبقى اكيد لازم يكون موجود في ال
+
+357
+00:26:37,150 --> 00:26:42,510
+media H2S indicator زي فحص ال SIM لكن ال H2S
+
+358
+00:26:42,510 --> 00:26:47,920
+indicator هو فرق امونيوم سيترات تمامفرق أمانيا
+
+359
+00:26:47,920 --> 00:26:51,000
+المسيطرات اللى موجود في هذه ال media ال H2S
+
+360
+00:26:51,000 --> 00:26:56,100
+Indicator طيب لو البكتيريا عملت gelicose
+
+361
+00:26:56,100 --> 00:26:59,120
+fermentation يبقى هيتكون ناتجة عملية ال
+
+362
+00:26:59,120 --> 00:27:04,580
+fermentation acid هيتحول لون البطل للون yellow صح
+
+363
+00:27:04,580 --> 00:27:09,220
+لكن لو البكتيريا عملت deamination للبيبطون هتنزع
+
+364
+00:27:09,220 --> 00:27:14,540
+الأمانيا البيبطون عبارة عن قلنا amino acidالأمانو
+
+365
+00:27:14,540 --> 00:27:17,960
+أسد الهيكلية تبعته في يندي أمونيا عملت دي
+
+366
+00:27:17,960 --> 00:27:21,340
+emination زلة هاي الأمونيا يبقى الأمونيا عندى
+
+367
+00:27:21,340 --> 00:27:26,360
+alkaline product هترفع ال pH هيتكون اللون هيتحول
+
+368
+00:27:26,360 --> 00:27:30,170
+لون ال media للون أحمرلو البكتيريا عندها القدرة
+
+369
+00:27:30,170 --> 00:27:34,990
+انها تعمل H2S production قلنا هيتكون H2S gas
+
+370
+00:27:34,990 --> 00:27:37,950
+هيرتبط مع ال indicator الموجود في ال media هيتكون
+
+371
+00:27:37,950 --> 00:27:41,650
+عندنا black precipitate في البط يبقى هك باعرف انه
+
+372
+00:27:41,650 --> 00:27:48,750
+صار فيه عندنا H2S production هذا بالنسبة لكف او اش
+
+373
+00:27:48,750 --> 00:27:53,650
+اللي بيصير في هاي ال media هلأ لو صار فيه عندنا او
+
+374
+00:27:53,650 --> 00:27:57,070
+اتكون لون يلو في البط برمز ليلو بالرمز A
+
+375
+00:28:06,490 --> 00:28:11,530
+لو أتكون لون أحمر في ال slant صار في عندي
+
+376
+00:28:11,530 --> 00:28:20,130
+deamination للبيبتون بارمز بالرمز K هنفهم
+
+377
+00:28:20,130 --> 00:28:25,430
+أكتر لما نقرأ النتائج أو نكتب النتيجة ال black
+
+378
+00:28:25,430 --> 00:28:27,630
+abrasive بتاعي بكتب H2S
+
+379
+00:28:35,780 --> 00:28:40,500
+لون ال media بيكون لون orange تمام فبالتالي لو
+
+380
+00:28:40,500 --> 00:28:44,200
+اتحول اللون للون يلو في ال pot يبقى هكذا لو صار في
+
+381
+00:28:44,200 --> 00:28:48,440
+عندي gelicose fermentation لو صار في عندي لون أحمر
+
+382
+00:28:48,440 --> 00:28:51,360
+في ال slant يبقى صار في عندي deamination للبيبطان
+
+383
+00:28:51,360 --> 00:28:55,500
+لو اتكون black precipitate معناه ان في عندي H2S
+
+384
+00:28:55,500 --> 00:28:56,060
+production
+
+385
+00:29:02,120 --> 00:29:08,300
+هلأ بالنسبة لكيف بيصير او كيف البكتيريا بتستهلك
+
+386
+00:29:08,300 --> 00:29:13,040
+المواد الغذائية هلأ كل البكتيريا اللى عندها القدرة
+
+387
+00:29:13,040 --> 00:29:16,820
+انها تعمل fermentation هتعمل fermentation لأبسط
+
+388
+00:29:16,820 --> 00:29:21,920
+سكر اللى هو الجليكوز يبقى روحت أنا زراعة البكتيريا
+
+389
+00:29:21,920 --> 00:29:26,820
+في high ال media أول مصدر رح تلجأ لإله البكتيريا
+
+390
+00:29:26,820 --> 00:29:31,020
+هو الجليكوزالموجود في ال media أكيد هيكون فيه
+
+391
+00:29:31,020 --> 00:29:34,280
+جليكوز بما أنني بدي أكشف على ال fermentation هيكون
+
+392
+00:29:34,280 --> 00:29:38,960
+موجود جليكوز لكن موجود بكمية قليلة يبقى أول مصدر
+
+393
+00:29:38,960 --> 00:29:44,640
+البكتيريا هتروح له هو الجليكوز لو البكتيريا عندها
+
+394
+00:29:44,640 --> 00:29:49,540
+القدرة أنها تعمل fermentation هتروح لجليكوز و تعمل
+
+395
+00:29:49,540 --> 00:29:53,730
+fermentation يبقى ال fermentation قلنالو عندها
+
+396
+00:29:53,730 --> 00:29:56,530
+البكتيريا القدرة انها تعمل fermentation هتروح
+
+397
+00:29:56,530 --> 00:30:01,370
+لأبسط سكر أول مرحلة خلال أول 12 ساعة هتروح
+
+398
+00:30:01,370 --> 00:30:06,450
+للجليكوز اللي هو أبسط سكرهتعمله fermentation يبقى
+
+399
+00:30:06,450 --> 00:30:11,050
+أول 12 ساعة هتلجأ البكتيريا للجليكوز وهتعمله تخمير
+
+400
+00:30:11,050 --> 00:30:18,670
+هيتكون acid حمض هيرمل على خفض البيئة هيتحول لون ال
+
+401
+00:30:18,670 --> 00:30:22,250
+media للون أصفر في البط قولنا ال fermentation
+
+402
+00:30:22,250 --> 00:30:24,950
+بيحصل في ال and European condition يبقى بيحصل في
+
+403
+00:30:24,950 --> 00:30:31,260
+البط هيتحول لون ال tube للون أصفر في البطلكن سعر
+
+404
+00:30:31,260 --> 00:30:34,060
+هو ال fermentation في الـun-aerobic condition لكن
+
+405
+00:30:34,060 --> 00:30:39,220
+اللون الأصفر بيمتد في كل tube سواء في البط أو سواء
+
+406
+00:30:39,220 --> 00:30:43,180
+في الإسلان يبقى ال fermentation في سير في ظروف لا
+
+407
+00:30:43,180 --> 00:30:47,580
+هوائية بيحول بما أن سعر ال fermentation هيتكون
+
+408
+00:30:47,580 --> 00:30:51,440
+acid ال acid هيعمل على خفض ال pH هيتحول لون ال
+
+409
+00:30:51,440 --> 00:30:55,360
+media للون ال tube هيمتد اللون الأصفر في كل tube
+
+410
+00:30:55,360 --> 00:31:00,440
+سواء في البط أو في الإسلانيبقى برمز لهذه النتيجة
+
+411
+00:31:00,440 --> 00:31:04,880
+على بما أنه هنتكوّن لون يلو و هان لون يلو يبقى A
+
+412
+00:31:04,880 --> 00:31:09,100
+يلو فوق بالإسلانت على A في البط يبقى A يلو فوق
+
+413
+00:31:09,100 --> 00:31:09,240
+بالإسلانت على A في البط يبقى A يلو فوق بالإسلانت
+
+414
+00:31:09,240 --> 00:31:12,620
+على A في البط يبقى A يلو فوق بالإسلانت على A في
+
+415
+00:31:12,620 --> 00:31:17,020
+البط يبقى A يلو فوق بالإسلانت على A في البط يبقى A
+
+416
+00:31:17,020 --> 00:31:20,020
+يلو فوق بالإسلانت على A في البط يبقى A يلو فوق
+
+417
+00:31:20,020 --> 00:31:21,160
+بالإسلانت على A في البط يبقى A يلو فوق بالإسلانت
+
+418
+00:31:21,160 --> 00:31:25,160
+على A في البط يبقى A يلو فوق بالإسلانت على A
+
+419
+00:31:26,620 --> 00:31:30,700
+الجليكوز قُلت موجود بكمية قليلة في الميديا موجود
+
+420
+00:31:30,700 --> 00:31:36,180
+بنسبة 1 من 10% بعد ما يخلص الجليكوز أكيد البكتيريا
+
+421
+00:31:36,180 --> 00:31:40,880
+هتروح لمصادر تانية موجود في الميديا قلنا لل triple
+
+422
+00:31:40,880 --> 00:31:45,880
+والكليجر iron agar قلنا موجود جليكوز وموجود
+
+423
+00:31:45,880 --> 00:31:51,190
+lactose فبالتالي البكتيريا هتروح لل lactoseلو
+
+424
+00:31:51,190 --> 00:31:54,370
+البكتيريا هذا خلال أول 12 ساعة قلنا البكتيريا
+
+425
+00:31:54,370 --> 00:31:58,110
+بتروح للجليكوز موجود بكمية قليلة هتروح بعدين
+
+426
+00:31:58,110 --> 00:32:03,150
+لمصادر تانية بقولي من خلال بعد 12 ل 24 ساعة
+
+427
+00:32:03,150 --> 00:32:07,270
+البكتيريا هتروح للكتوز لو البكتيريا عندها القدرة
+
+428
+00:32:07,270 --> 00:32:12,030
+انها تخمر اللكتوز او الساكراز لو ال media لو ال
+
+429
+00:32:12,030 --> 00:32:14,770
+media هي ال triple sugar iron agar لو البكتيريا
+
+430
+00:32:14,770 --> 00:32:18,150
+عندها القدرة انها تعمل fermentation لالهم ايش
+
+431
+00:32:18,150 --> 00:32:25,670
+هيصير؟هتعمل تخمير لباقي السكريات هينتج جمية كبيرة
+
+432
+00:32:25,670 --> 00:32:30,990
+من ال acid من الحمض هيعمل على طبعا خفض ال pH راح
+
+433
+00:32:30,990 --> 00:32:34,330
+يظل كل tube لونه أصفر يبقى هتظلها النتيجة نفس
+
+434
+00:32:34,330 --> 00:32:37,510
+الشيء yellow في ال slant و yellow في ال butt يبقى
+
+435
+00:32:37,510 --> 00:32:42,070
+النتيجة a على a a على اللي هي ال slant على a في ال
+
+436
+00:32:42,070 --> 00:32:46,650
+butt يبقى هيتكون جمية acid بصورة كبيرة هيظل ال
+
+437
+00:32:46,650 --> 00:32:50,650
+tube كله لونه أصفرطب لو البكتيريا ما عندها القدرة
+
+438
+00:32:50,650 --> 00:32:55,410
+على تخمير باقي السكريات زي اللاكتوز او السكاروز
+
+439
+00:32:55,410 --> 00:33:00,250
+اكيد هتلجأ لمصادر اخرى احنا قلنا اولا الجليكوز
+
+440
+00:33:00,250 --> 00:33:05,910
+بعدين اللاكتوز او السكاروز مش موجودين هتروح لمصادر
+
+441
+00:33:05,910 --> 00:33:09,310
+اخرى المصدر اللى بعده اللى هتروح البكتيريا عليه
+
+442
+00:33:09,310 --> 00:33:14,560
+اللى هو البيبطان البيبطان موجود في ال mediaهتعمله
+
+443
+00:33:14,560 --> 00:33:17,920
+الـ deamination للبيبتون قولنا هيتكون الأمانيا
+
+444
+00:33:17,920 --> 00:33:21,720
+الأمانيا alkaline product ال alkaline product
+
+445
+00:33:21,720 --> 00:33:26,320
+هيتحول نتيجة اللي هو وجود الفيه نار red للون لون
+
+446
+00:33:26,320 --> 00:33:30,880
+ال media هيتحول للون red في ال slant فقط في ال
+
+447
+00:33:30,880 --> 00:33:36,580
+slant قولنا هيتحول لون ال media للون أحمر لأنه
+
+448
+00:33:36,580 --> 00:33:40,800
+احنا قولنا ان ال deamination للبيبتون هذا هيصير
+
+449
+00:33:40,800 --> 00:33:46,700
+فقط فيظروف هوائية في الـ aerobic condition فقط
+
+450
+00:33:46,700 --> 00:33:49,780
+هيصير في الـ slant يبقى هيتحول لون ال slant فقط
+
+451
+00:33:49,780 --> 00:33:55,040
+للون red لكن ال butt هيظل لونه أصفر يبقى هان نحكي
+
+452
+00:33:55,040 --> 00:34:00,120
+لون red أحمر و لون ال butt لون ال butt هيظل لون
+
+453
+00:34:00,120 --> 00:34:05,660
+أصفر يبقى red على yellow red قلنا نرمز لإله بالرمز
+
+454
+00:34:05,660 --> 00:34:11,910
+K يبقى K على A هيك نرمز النتيجة K على Aهذا بالنسبة
+
+455
+00:34:11,910 --> 00:34:16,170
+لو البكتيريا هيك قرأت او هيك الفحص هيك النتيجة
+
+456
+00:34:16,170 --> 00:34:20,250
+بتكون لو البكتيريا جيليكوز fermentation لكن non
+
+457
+00:34:20,250 --> 00:34:23,990
+-lactose fermenter بتكون red على yellow لكن لو
+
+458
+00:34:23,990 --> 00:34:28,050
+كانت جيليكوز و lactose fermentation بتكون yellow
+
+459
+00:34:28,050 --> 00:34:33,330
+على yellow ا على ا هذا بعد 24 ساعة لكن خلال أول 12
+
+460
+00:34:33,330 --> 00:34:42,970
+ساعة لو كانت lactoseأو non-lactose fermenter ستكون
+
+461
+00:34:42,970 --> 00:34:48,130
+A على A عشان هي كم نقرأ النتيجة عند 24 ساعة هدنا
+
+462
+00:34:48,130 --> 00:34:53,470
+هنتكلم في هذا الموضوع بالنسبة لو البكتيريا كانت
+
+463
+00:34:53,470 --> 00:34:57,740
+بالاصل non-gelicose fermenter هذا اتكلمنا عنههذا
+
+464
+00:34:57,740 --> 00:35:00,940
+كله لو كانت البكتيريا في الأصل هي Gelicose
+
+465
+00:35:00,940 --> 00:35:04,540
+Fermenter لكن لو البكتيريا هي ماعندها القدرة انها
+
+466
+00:35:04,540 --> 00:35:08,320
+تعمل fermentation لأبسط سكر الجليكوز يبقى أكيد مش
+
+467
+00:35:08,320 --> 00:35:12,840
+هتعمل fermentation للسكر الثنائي اللكتاز والسكراز
+
+468
+00:35:12,840 --> 00:35:17,680
+فبالتالي هتروح لمين هتروح للبيبتون هتعمله
+
+469
+00:35:17,680 --> 00:35:21,900
+deamination هيتكون عندي alkaline product هيتحول
+
+470
+00:35:21,900 --> 00:35:25,910
+لون ال media للون redهيصير اللون الـ Red قلنا الـ
+
+471
+00:35:25,910 --> 00:35:30,050
+Deamination للبيبتون هيصير في الـ Slant في الـ
+
+472
+00:35:30,050 --> 00:35:33,870
+Aerobic condition يبقى هيتحول اللون الـ Slant للون
+
+473
+00:35:33,870 --> 00:35:37,850
+الـ Red لكن البط مش هيصله أي تغير No, it changed
+
+474
+00:35:37,850 --> 00:35:41,770
+بقوللي ممكن برضه لو البكتيريا في الأصل non
+
+475
+00:35:41,770 --> 00:35:45,430
+-gelicose fermenter ممكن إنها لو ما عندها القدرة
+
+476
+00:35:45,430 --> 00:35:48,110
+إنها تعمل للـ Gelicose فبتعرفش هتعمل للاكتوز ولا
+
+477
+00:35:48,110 --> 00:35:53,880
+للسكراز أكيد هتعمل Deamination للبيبتون فيالـ
+
+478
+00:35:53,880 --> 00:35:58,380
+aerobic condition في الظروف الهوائية هيتحول لون
+
+479
+00:35:58,380 --> 00:36:02,260
+الـ slant للون red طيب لو البكتيريا ما اكتفت
+
+480
+00:36:02,260 --> 00:36:07,000
+بحاجتها من المواد الغذائية بقوللي هتعمل
+
+481
+00:36:07,000 --> 00:36:11,340
+deamination للبيبتون في ظروف unaerobic condition
+
+482
+00:36:11,340 --> 00:36:17,360
+بشكل اضطراري يبقى هان صار عند deamination للبيبتون
+
+483
+00:36:17,360 --> 00:36:22,560
+في ظروف unaerobic condition لكنبشكل اضطراري يبقى
+
+484
+00:36:22,560 --> 00:36:25,440
+لو كانت البكتيريا non-gelicose fermenter ممكن تكون
+
+485
+00:36:25,440 --> 00:36:32,400
+red على not change نكتب K على not change أو red
+
+486
+00:36:32,400 --> 00:36:39,860
+على red اللي هي K على K يبقى هان النتيجة نكتبها K
+
+487
+00:36:49,430 --> 00:36:54,710
+هان K على not change لأنه احنا قلنا ال red نرمز له
+
+488
+00:36:54,710 --> 00:37:14,710
+بالرمز K alkaline اتكون و هان K على K طيب
+
+489
+00:37:15,350 --> 00:37:18,250
+هذا بالنسبة لو كانت البكتيريا non-glycose
+
+490
+00:37:18,250 --> 00:37:22,490
+fermenter إما تكون K على K أو ممكن تكون K على نهار
+
+491
+00:37:22,490 --> 00:37:28,610
+تتشانج وفسرنا كل نتيجة إيش بتكون بالظبط طيب نوادج
+
+492
+00:37:28,610 --> 00:37:31,970
+عملية ال fermentation قلنا إما أسد واتكلمنا إنه
+
+493
+00:37:31,970 --> 00:37:38,310
+بتكون لون يلو فيه لتيوب أو جاز طيب بالنسبة للجاز
+
+494
+00:37:38,310 --> 00:37:43,350
+كيف بدي أكشف عنه قلنا إن الجاز ممكن كنا نكشف عنه
+
+495
+00:37:43,350 --> 00:37:47,690
+عن طريق الدرهام التيوبلكن الدرهام تيوب فقط بقدر
+
+496
+00:37:47,690 --> 00:37:52,210
+احطها لو كانت ال media broth هاي slant slant أجار
+
+497
+00:37:52,210 --> 00:37:55,790
+it's you بيبقى في عندي نسبة أجار ما بقدر احط درهام
+
+498
+00:37:55,790 --> 00:37:59,850
+it's you كيف بدي أكشف على النواتج عملية ال
+
+499
+00:37:59,850 --> 00:38:03,730
+fermentation لو كان في عندي تكون gas بقوللي بتكون
+
+500
+00:38:03,730 --> 00:38:07,890
+cracks بتكون زي تشققات في ال media او ممكن ال
+
+501
+00:38:07,890 --> 00:38:12,210
+media تطلع لفوق هيك بعرف ان والله اتكون عندي gas
+
+502
+00:38:12,210 --> 00:38:19,150
+طيببالنسبة للـ H2S production الـ H2S production
+
+503
+00:38:19,150 --> 00:38:23,650
+احنا قلنا انه الهاي الميديا بتكشف على ال
+
+504
+00:38:23,650 --> 00:38:27,090
+fermentation بتكشف على ال deamination للبيبتون
+
+505
+00:38:27,090 --> 00:38:30,410
+قلنا برضه ال fermentation ممكن يكون acid و gas
+
+506
+00:38:30,410 --> 00:38:33,950
+وعرفنا كيف كل نتيجة أشبهون لونها أو كيف بيكون
+
+507
+00:38:33,950 --> 00:38:39,450
+الشكل لكن بالنسبة لـالـ H2S production طيب لو تم
+
+508
+00:38:39,450 --> 00:38:43,390
+إنتاج اللون الأسود احنا قلنا الـ H2S production
+
+509
+00:38:43,390 --> 00:38:48,830
+بتكون عندي black precipitate هل هقدر أشوف طبعا الـ
+
+510
+00:38:48,830 --> 00:38:57,850
+H2S production بيصير زي ما قلنا قبل الـ
+
+511
+00:38:57,850 --> 00:39:01,230
+H2S production قلنا فيه بيصير في ظروف أن European
+
+512
+00:39:01,230 --> 00:39:06,620
+condition بيكون في البطلو صار في عندي إنتاج اللون
+
+513
+00:39:06,620 --> 00:39:11,480
+الأسود هل هقدر أشوف اللون الأصفر الناتج من عملية
+
+514
+00:39:11,480 --> 00:39:14,400
+ال fermentation خاصة أن ال fermentation و ال H2S
+
+515
+00:39:14,400 --> 00:39:17,420
+production بيصيروا في الـ Un-aerobic condition
+
+516
+00:39:17,420 --> 00:39:22,300
+بيصيروا في البط لو صار في عندي تكون للون الأسود
+
+517
+00:39:22,300 --> 00:39:27,280
+الناتج من أنه اتكون ال H2S gas هل هقدر أشوف اللون
+
+518
+00:39:27,280 --> 00:39:33,300
+الأصفر في البط ولا لأ؟ بقولليلو عملت البكتيريا H2S
+
+519
+00:39:33,300 --> 00:39:39,120
+production بدي أكون عارفة إنه عملت Gelicose
+
+520
+00:39:39,120 --> 00:39:43,280
+fermentation يبقى طالما البكتيريا H2S production
+
+521
+00:39:43,280 --> 00:39:46,760
+positive يبقى هي Gelicose fermentation positive
+
+522
+00:39:46,760 --> 00:39:53,020
+لأن إنتاج الـH2S production يحصل في الوسط الحمضي
+
+523
+00:39:53,280 --> 00:39:58,480
+الناتج من عملية تخمير اللي هو السكريات فبالتالي و
+
+524
+00:39:58,480 --> 00:40:01,900
+اتكون acid صار في عندي acid ال condition هيتكون ال
+
+525
+00:40:01,900 --> 00:40:07,200
+H2S production يبقى بدنا نكون عارفين إذا اتكون عند
+
+526
+00:40:07,200 --> 00:40:11,160
+ال H2S لون اتكون لون أسود في البط على طول بكتوب
+
+527
+00:40:11,160 --> 00:40:16,240
+جنبها ان صار في عندي جليكوز fermentation لأن عملية
+
+528
+00:40:16,240 --> 00:40:21,260
+ال H2S production بتصير في الوسط الحمضي هذا
+
+529
+00:40:21,260 --> 00:40:22,180
+بالنسبة ل
+
+530
+00:40:26,150 --> 00:40:30,810
+الـ H2S production يبقى عريفة انها كل عملية عشان
+
+531
+00:40:30,810 --> 00:40:33,610
+نتيجة دي ammunition للبيبتون قولنا بيكون في عندي
+
+532
+00:40:33,610 --> 00:40:37,830
+اتكوين لون Red في ال slant ممكن يصير بشكل اضطراري
+
+533
+00:40:37,830 --> 00:40:42,690
+في البط ال fermentation لو صار في عندي لجليكوز فقط
+
+534
+00:40:42,690 --> 00:40:46,830
+جليكوز fermentation بدون ما يصير للاكتوز والسكراز
+
+535
+00:40:46,830 --> 00:40:51,890
+بتكون عندي في البط لون أصفر لكن في ال slant بيكون
+
+536
+00:40:51,890 --> 00:40:56,000
+في عنديلون أحمر نتيجة الـdeamination للـpeptide
+
+537
+00:40:56,000 --> 00:40:58,760
+لكن لو البكتيريا عندها القدرة إنها تعمل
+
+538
+00:40:58,760 --> 00:41:01,180
+fermentation لكل السكريات الموجودة في الـmedia
+
+539
+00:41:01,180 --> 00:41:05,180
+هلاقي كل tube لونه أصفر الـH2O acid production
+
+540
+00:41:05,180 --> 00:41:10,660
+قولنا لو اتكون راسب أسود في البط يبقى هذا معناه
+
+541
+00:41:10,660 --> 00:41:13,320
+إنه بدي أعرف إنه والله صار في عندي جليكوز
+
+542
+00:41:13,320 --> 00:41:17,500
+fermentation already لأن الـH2O acid production
+
+543
+00:41:17,500 --> 00:41:19,280
+بيصير في الـacidic condition
+
+544
+00:41:25,030 --> 00:41:32,450
+طيب فيها معلومة مهمة ان انا بقرأ النتائج لل triple
+
+545
+00:41:32,450 --> 00:41:38,910
+sugar iron agar عند 24 ساعة ممنوع اقرأ بعد 24 ساعة
+
+546
+00:41:38,910 --> 00:41:45,270
+و ممنوع اقرأ قبل 24 ساعة طيب لو قرأت بعد 24 ساعة
+
+547
+00:41:45,270 --> 00:41:49,110
+بقولي كل الكربوهيدرات الموجودة في ال media هتكون
+
+548
+00:41:49,110 --> 00:41:55,810
+تم استهلاكها will be depleted ال bacteria لووبرضه
+
+549
+00:41:55,810 --> 00:42:00,210
+البكتيريا هتلجأ بما انه خلصت بعد 24 ساعة هيكون خلص
+
+550
+00:42:00,210 --> 00:42:03,910
+الجليكوز already هتكون خلص اللاكتوز والسكراز
+
+551
+00:42:03,910 --> 00:42:08,030
+البكتيريا هتلجأ لمصادر تانية اللي هو ال بيبتون
+
+552
+00:42:08,030 --> 00:42:14,010
+وهتعمله دي ammunition فبالتالي لو كانت النتيجة A
+
+553
+00:42:14,010 --> 00:42:17,850
+على A لو كانت البكتيريا في الأصل جليكوز fermenter
+
+554
+00:42:17,850 --> 00:42:21,130
+عندها القدرة على تخمير اللاكتوز والسكراز قلنا
+
+555
+00:42:21,130 --> 00:42:25,220
+بتكون النتيجة A على Aبتعمل fermentation للجليكوز
+
+556
+00:42:25,220 --> 00:42:30,120
+خلال 12 ساعة خلال أول 12 ساعة بعد هيخلص الجليكوز
+
+557
+00:42:30,120 --> 00:42:33,160
+لأنه موجود في كمية قليلة هتروح للكتاز والسكراز
+
+558
+00:42:33,160 --> 00:42:37,320
+هينتج كمية أسد كبيرة هينتشر في كل tube اللون
+
+559
+00:42:37,320 --> 00:42:41,140
+الأصفر وهيتكون yellow في ال slant و yellow في البط
+
+560
+00:42:41,140 --> 00:42:45,680
+يبقى هتكون النتيجة a على a لو كانت النتيجة a على a
+
+561
+00:42:45,680 --> 00:42:50,950
+تمام هي بالاصل lactose fermenterورحت أنا قرأت
+
+562
+00:42:50,950 --> 00:42:55,570
+النتيجة بعد 24 ساعة بقول لي انه اكيد بعد 24 ساعة
+
+563
+00:42:55,570 --> 00:43:00,510
+هيخلص اللكتاز والسكراز هتروح البكتيريا لمصدر تاني
+
+564
+00:43:00,510 --> 00:43:03,750
+اللي هو ال بيبتون هتعمله deamination ال
+
+565
+00:43:03,750 --> 00:43:06,770
+deamination للبيبتون قلنا بيصير في ظروف europic
+
+566
+00:43:06,770 --> 00:43:11,830
+condition بيصير في الإسلاند بيتحول لون الإسلاند
+
+567
+00:43:11,830 --> 00:43:17,210
+للون red يبقى هتتحول النتيجة ال a على a ل k على a
+
+568
+00:43:17,210 --> 00:43:22,290
+فهفكرها أناإنه هي non-lactose fermenter لكن هي في
+
+569
+00:43:22,290 --> 00:43:25,510
+الأصل lactose fermenter لكن لأني أنا قرأت النتيجة
+
+570
+00:43:25,510 --> 00:43:32,790
+بعد 24 ساعة طيب لو قرأت قبل 24 ساعة بقولي لو قرأتي
+
+571
+00:43:32,790 --> 00:43:36,130
+على سبيل المثال كانت البكتيريا هي non-lactose or
+
+572
+00:43:36,130 --> 00:43:40,310
+saccharose fermenter كانت K على A وروحت أنا قرأتها
+
+573
+00:43:40,670 --> 00:43:45,010
+قبل 24 ساعة قبل 24 ساعة قلنا سواء كانت lactose
+
+574
+00:43:45,010 --> 00:43:47,970
+fermenter أو non-lactose fermenter بتكون A على A
+
+575
+00:43:47,970 --> 00:43:51,790
+لأنه فقط بيتم استهلاك الجليكوز خلال أول 12 ساعة
+
+576
+00:43:51,790 --> 00:43:57,250
+بيتحول بيتكون acid هذا النواتج عملية ال
+
+577
+00:43:57,250 --> 00:44:00,310
+fermentation acid بيصير في الان European condition
+
+578
+00:44:00,310 --> 00:44:06,190
+لكن اللون الأصفر بيمتد في كل tube فبالتالي هفكر
+
+579
+00:44:06,190 --> 00:44:12,110
+النتيجة A على Aيبقى لو أنا قرأت قبل 24 ساعة وكانت
+
+580
+00:44:12,110 --> 00:44:15,390
+البكتيريا بالاصل هي non-lactose fermenter يبقى
+
+581
+00:44:15,390 --> 00:44:19,630
+النتيجة المفترض أنا أقرأها K على A لو قرأتها قبل
+
+582
+00:44:19,630 --> 00:44:23,610
+24 ساعة هتكون النتيجة A على A فبالتالي هيصير في
+
+583
+00:44:23,610 --> 00:44:27,050
+عندي false result عشان هيك لازم أقرأ النتيجة عند
+
+584
+00:44:27,050 --> 00:44:33,070
+24 ساعة مش قبل 24 ساعة و مش بعد 24 ساعة قلنا بعد
+
+585
+00:44:33,070 --> 00:44:37,490
+24 ساعة لو كانت البكتيريا A على A هتبين عندي كأنها
+
+586
+00:44:37,490 --> 00:44:42,800
+K على Aلكن لو قرأت قبل 24 ساعة و كانت في الأصل هي
+
+587
+00:44:42,800 --> 00:44:46,760
+K على A هتبين عندي كأنها A على A فبالتالي كل
+
+588
+00:44:46,760 --> 00:44:48,880
+النتائج هتكون عندي غلط
+
+589
+00:44:54,580 --> 00:44:58,900
+نرجع على السلايدات kliger iron agar اللي kliger هو
+
+590
+00:44:58,900 --> 00:45:02,360
+اسم العالم iron لأنه بدي أكشف فيها على الـ H2O
+
+591
+00:45:02,360 --> 00:45:06,880
+production agar لأنه أكيد في الميديا هي فيها نسبة
+
+592
+00:45:06,880 --> 00:45:11,360
+agar بنسبة واحد ونص في المية ال triple sugar iron
+
+593
+00:45:11,360 --> 00:45:15,280
+agar لأنه ال triple فيها تلت سكريات قولنا ال
+
+594
+00:45:15,280 --> 00:45:20,080
+dextrose اللي هو الجليكوز أو اللكتوز والسكروزIron
+
+595
+00:45:20,080 --> 00:45:23,460
+بدي أكشف على الـ H2S production و الأجار لأن فيها
+
+596
+00:45:23,460 --> 00:45:27,600
+نسبة أجار واحد و نصف في الميديا واحد و نصف بالمية
+
+597
+00:45:27,600 --> 00:45:31,780
+Cligar iron agar and triple sugar iron agar are
+
+598
+00:45:31,780 --> 00:45:36,140
+widely used in the identification of gram negative
+
+599
+00:45:36,140 --> 00:45:40,500
+bacteria particularly the enterobacteriasis هذه
+
+600
+00:45:40,500 --> 00:45:45,420
+الميديا بتستخدم للتعريف للgram negative bacteria
+
+601
+00:45:45,420 --> 00:45:48,230
+خاصة ال enterobacteriasisقلنا ان الـ
+
+602
+00:45:48,230 --> 00:45:51,570
+Enterobacteriasis هي الجرام نيجاتيف بسلاي تعتبر
+
+603
+00:45:51,570 --> 00:45:56,670
+enteric بكتيريا بكشف من خلال أو بعمل تعريف للـ
+
+604
+00:45:56,670 --> 00:45:59,590
+Enterobacteriasis من خلال triple sugar iron agar
+
+605
+00:45:59,590 --> 00:46:03,870
+أو كليجر ايرون اجار هذه ال media the media are
+
+606
+00:46:03,870 --> 00:46:08,150
+identical الميديتين بتشابه بعض نفس الاشي بيتشابه
+
+607
+00:46:08,150 --> 00:46:11,710
+لكن الاختلاف ان ال triple sugar iron agar بتحتوي
+
+608
+00:46:11,710 --> 00:46:15,970
+على استكاروزهو الإختلاف بالإضافة للـ Dextrose والـ
+
+609
+00:46:15,970 --> 00:46:20,930
+Lactose الموجود في الجليجر ايرون اجار كيف انا
+
+610
+00:46:20,930 --> 00:46:25,170
+بصبها ال media قلنا ال media بصبها slant تمام كيف
+
+611
+00:46:25,170 --> 00:46:29,630
+بزرعها بعمل stabbing لل بط للبط بعمل stabbing
+
+612
+00:46:29,630 --> 00:46:33,310
+بعدين بعمل بطلة على ال slant surface و بعمل زيجا
+
+613
+00:46:33,310 --> 00:46:37,530
+زاج هك احنا قلنا ان نزرع ال slant tube هك
+
+614
+00:46:37,530 --> 00:46:41,470
+البكتيريا بتتعرض ل anaerobicEnvironment تقولنا
+
+615
+00:46:41,470 --> 00:46:45,890
+بتتعرض لظروف لا هوائية من خلال البط وظروف هي
+
+616
+00:46:45,890 --> 00:46:50,090
+هوائية اللي هي الـAerobic من خلال الاسلاند الفينار
+
+617
+00:46:50,090 --> 00:46:53,870
+Red موجود ك Indicator ك BH Indicator في ال media
+
+618
+00:46:53,870 --> 00:46:57,210
+وقلنا في عندي كمان H2S Indicator اللي هو الferric
+
+619
+00:46:57,210 --> 00:47:01,030
+ammonium citrate لو البكتيريا في الأصل هي non
+
+620
+00:47:01,030 --> 00:47:04,310
+-fermenter ما بتعمل fermentation للجليكوز ولا
+
+621
+00:47:04,310 --> 00:47:10,090
+للكتاز ولا للسكراز أكيد هتلجأ لمصدر البيبتونزي الـ
+
+622
+00:47:10,090 --> 00:47:13,170
+Sodomonas احنا قلنا Sodomonas تعتبر non-gelicose
+
+623
+00:47:13,170 --> 00:47:18,230
+fermenter فبالتالي هتنجأ للبيبتون هتنمو فقط على ال
+
+624
+00:47:18,230 --> 00:47:22,230
+slant تمام لأنه احنا قلنا انه هي Sodomonas
+
+625
+00:47:22,230 --> 00:47:25,830
+obligate aerobic bacteria فبالتالي هتنمو على ال
+
+626
+00:47:25,830 --> 00:47:29,970
+slant في ال aerobic condition هتعمل degradation
+
+627
+00:47:29,970 --> 00:47:34,450
+للبروتين للبيبتون قلنا هتعمل deamination للبيبتون
+
+628
+00:47:34,450 --> 00:47:39,360
+هيتكون alkaline product هيتحولاللون ال media للون
+
+629
+00:47:39,360 --> 00:47:46,080
+أحمر في ال slant فقط فبالتالي هيك بتكون ال اللي هي
+
+630
+00:47:46,080 --> 00:47:50,080
+نتيجة ال Sodomonas انه صار في عندي deamination
+
+631
+00:47:50,080 --> 00:47:56,440
+للبيبتون هيتحول لون ال slant للون أحمر مكونات ال
+
+632
+00:47:56,440 --> 00:47:59,480
+media مكونات ال media في عندي enzymatic digest of
+
+633
+00:47:59,480 --> 00:48:02,760
+غازين enzymatic digest of animal tissue هدول
+
+634
+00:48:02,760 --> 00:48:06,990
+يعتبروامصادر للتغذية مصدر لل كربون وال نيتروجن
+
+635
+00:48:06,990 --> 00:48:10,290
+source اللازم لنمو البكتيريا هاي لون ال media
+
+636
+00:48:10,290 --> 00:48:18,030
+لونها جاي على أورنج تمام يست انرشت بيبتون قولنا
+
+637
+00:48:18,030 --> 00:48:23,770
+البيبتون يعتبر كمصدر للهو ال nitrogen source وكمان
+
+638
+00:48:23,770 --> 00:48:27,580
+لل carbon sourceوقلنا عشان البكتيريا تعمله بدي
+
+639
+00:48:27,580 --> 00:48:30,260
+أكشف على الـ deamination للبيبطان فبالتالي أكيد
+
+640
+00:48:30,260 --> 00:48:34,000
+لازم يكون في عيندي بيبطان في الميديا ديكستراز
+
+641
+00:48:34,000 --> 00:48:38,300
+واللاكتاز موجودين في ال triple وكلجر ايرون اجار
+
+642
+00:48:38,300 --> 00:48:41,640
+قلنا الديكستراز موجود في كمية قليلة زي ما ملاحظين
+
+643
+00:48:41,640 --> 00:48:47,340
+1 من 10% اللاكتاز والسكراز موجود بكمية أعلى 1%
+
+644
+00:48:47,340 --> 00:48:50,520
+السكراز مش موجود في الكلجر ايرون اجار قلنا
+
+645
+00:48:50,520 --> 00:48:54,930
+الإختلاف بين الكلجر و ال tripleإنه استكالوز مش
+
+646
+00:48:54,930 --> 00:48:58,650
+موجود في الجليجر لكن موجود في ال triple sugar iron
+
+647
+00:48:58,650 --> 00:49:03,430
+agar في اندفيرك أمونيوم سايتريات اللي هو ال H2S
+
+648
+00:49:03,430 --> 00:49:07,510
+indicator صوديوم كلورايد عشان يحافظلي على ال
+
+649
+00:49:07,510 --> 00:49:10,470
+osmotic pressure صوديوم ثيو سلفات ال substrate
+
+650
+00:49:10,470 --> 00:49:13,950
+اللي هيشتغل عليها أنزيم الصوديوم ثيو سلفات
+
+651
+00:49:13,950 --> 00:49:18,090
+productive عشان أكشف على ال H2S production في نار
+
+652
+00:49:18,090 --> 00:49:21,990
+red قلنا اللي هو ال BH indicator قلنا بيكونفي ال
+
+653
+00:49:21,990 --> 00:49:28,150
+media لونه بيكون ال في ال طبعا في ال neutral لونه
+
+654
+00:49:28,150 --> 00:49:32,470
+orange وهو لون ال pH للميديا بدون ما أنا أزرعها
+
+655
+00:49:32,470 --> 00:49:35,990
+بتكون سبعة وأربع فبالتالي هنا سبعة وأربع بيكون لون
+
+656
+00:49:35,990 --> 00:49:42,130
+orange يبقى لون ال media هو لون orange لون برتقالي
+
+657
+00:49:42,130 --> 00:49:46,390
+الأجار أكيد بما أنه هي إسلانت يبقى نسبة الأجار
+
+658
+00:49:46,390 --> 00:49:52,230
+واحد ونصف في الميةهان ذكرلي كل مكوّن من المكونات
+
+659
+00:49:52,230 --> 00:49:55,470
+إيش؟ فإنتوا قولنا ال casein ال animal tissue ال
+
+660
+00:49:55,470 --> 00:49:59,530
+yeast and the restعشان اوفر اللي هو الـ nitrogen و
+
+661
+00:49:59,530 --> 00:50:02,010
+الـ carbon و الـ vitamin و الـ source اللي لازم
+
+662
+00:50:02,010 --> 00:50:05,330
+لنمو البكتيريا قولنا اللي triple sugar iron agar
+
+663
+00:50:05,330 --> 00:50:08,330
+بتحتوي على تلت أنواع من السكريات ديكستروز، لكتاز،
+
+664
+00:50:08,330 --> 00:50:11,710
+سكراز، لكن الـkliger iron agar فقط ديكستروز و
+
+665
+00:50:11,710 --> 00:50:14,770
+لكتاز لو البكتيريا عندها القدرة انها تعمل
+
+666
+00:50:14,770 --> 00:50:19,050
+fermentation لهاي السكريات هيتكون acid بقدر اكشف
+
+667
+00:50:19,050 --> 00:50:22,030
+عنه من خلال وجود الـ phenol red اللي هو الـ pH
+
+668
+00:50:22,030 --> 00:50:25,130
+indicatorالسوديوم سيوسالفات هو الـ substrate اللى
+
+669
+00:50:25,130 --> 00:50:29,550
+هيشتغل عليه الانزيم هيعمل لو موجود فيه البكتيريا
+
+670
+00:50:29,550 --> 00:50:33,630
+هيعمله اختيزة ل hydrogen sulfide ال hydrogen
+
+671
+00:50:33,630 --> 00:50:38,430
+sulfide هيتفاعل مع ال iron salt فبالتالي هيتكون
+
+672
+00:50:38,430 --> 00:50:42,600
+عندي black precipitate زى ما ذكرناه فيوشرحناها في
+
+673
+00:50:42,600 --> 00:50:46,720
+ميديا SIM Ferric Ammonium Citrate هو الـ H2S
+
+674
+00:50:46,720 --> 00:50:50,080
+Indicator اللي هيرتبط مع الـ H2S Gas وهيعطينا
+
+675
+00:50:50,080 --> 00:50:53,580
+black precipitate Sodium Chloride أي ميديا فيها
+
+676
+00:50:53,580 --> 00:50:56,520
+Sodium Chloride عشان يحافظ على الـ Osmotic
+
+677
+00:50:56,520 --> 00:51:03,840
+Pressure الأجار يعتبر ك solidifying agentهذه
+
+678
+00:51:03,840 --> 00:51:09,180
+الميديا بتعملي identification لـ Antirobacteriasi
+
+679
+00:51:09,180 --> 00:51:13,360
+من خلال هذه الميديا بكشف على قدرة البكتيريا انها
+
+680
+00:51:13,360 --> 00:51:19,300
+اتكون او انه اتكون gas نتيجة fermentation للشجر
+
+681
+00:51:19,300 --> 00:51:24,380
+انها اتعمل fermentation لللاكتوز او السكاروز كمان
+
+682
+00:51:24,380 --> 00:51:28,140
+انها اتكون اللي هو ال hydrogen sulfide الـH2S gas
+
+683
+00:51:33,190 --> 00:51:37,110
+principle for fermentative organism jelly cause is
+
+684
+00:51:37,110 --> 00:51:41,670
+the first sugar catabolized بقوللي في هاي ال media
+
+685
+00:51:41,670 --> 00:51:45,210
+انا بكشف على ال fermentation احنا قولنا ان في هاي
+
+686
+00:51:45,210 --> 00:51:48,950
+ال media انا بكشف على ال fermentation هلأ
+
+687
+00:51:48,950 --> 00:51:54,310
+البكتيريا هتستهلك اول ما هتروح هتروح لأبسط الطرق
+
+688
+00:51:54,310 --> 00:51:59,570
+حتى تحصل على الطاقة والغذاء فأكيد هتبدأ بأبسط مركب
+
+689
+00:51:59,570 --> 00:52:02,730
+اللي هو الجليكواز هتبدأ بال Dextrose اللي احنا
+
+690
+00:52:02,730 --> 00:52:07,890
+قلناه يعتبر simple sugar هتعمله fermentation لو
+
+691
+00:52:07,890 --> 00:52:10,690
+البكتيريا عندها القدرة انها تعمل fermentation لهذا
+
+692
+00:52:10,690 --> 00:52:14,990
+الجليكواز هتعمل fermentationهيتكون عندى acid
+
+693
+00:52:14,990 --> 00:52:20,470
+فبالتالي هيتحول لون ال media للون أصفر if glycose
+
+694
+00:52:20,470 --> 00:52:24,130
+is used up اذا تم استهلاك الجليكوز a bacterium
+
+695
+00:52:24,130 --> 00:52:27,230
+reach a metabolic fork in the rod البكتيريا هتوصل
+
+696
+00:52:27,230 --> 00:52:33,770
+للحظة ان هي هتكون زى الشوكة في الطريقتمام هذا
+
+697
+00:52:33,770 --> 00:52:37,130
+بالنسبة لأول إشي ان احنا قلنا ان البكتيريا هتروح
+
+698
+00:52:37,130 --> 00:52:43,430
+لأول مصدر اللي هو الجليكوز هتستهلك الجليكوز هيتكون
+
+699
+00:52:43,430 --> 00:52:47,870
+أسد هيتحول لون ال media فيه البط وهينتشر كمان في
+
+700
+00:52:47,870 --> 00:52:51,990
+ال إسلانت للون يلو if an organism produce the
+
+701
+00:52:51,990 --> 00:52:56,550
+enzyme to hydrolyze this saccharide lactose the
+
+702
+00:52:56,550 --> 00:53:01,050
+organism will continue fermenting to assist in ATP
+
+703
+00:53:01,050 --> 00:53:06,320
+generationبقول لي لو البكتيريا لو خلص طبعا اكيد ال
+
+704
+00:53:06,320 --> 00:53:10,580
+ال جيليوكوز موجود بكمية قليلة لو خلص ال dextrose
+
+705
+00:53:10,580 --> 00:53:14,980
+هتروح تستهلك اللكتوز او السكاروز او الاتنين معاها
+
+706
+00:53:14,980 --> 00:53:19,120
+حسب الانزيمات الموجودة عند البكتيريا تمام هذا
+
+707
+00:53:19,120 --> 00:53:26,140
+بالنسبة للكتوز و السكاروز however if the gelicose
+
+708
+00:53:26,140 --> 00:53:29,440
+is limiting and the organism doesn't produce them
+
+709
+00:53:29,720 --> 00:53:32,940
+necessary enzyme to catabolize lactose the
+
+710
+00:53:32,940 --> 00:53:36,780
+organism can utilize the protein in the media via
+
+711
+00:53:36,780 --> 00:53:41,680
+the process of deamination بقولي لو الجليكوز خلص
+
+712
+00:53:41,680 --> 00:53:44,740
+والبكتيريا ماعندها القدرة انها تكسر انزيمات
+
+713
+00:53:44,740 --> 00:53:47,280
+اللاكتوز يعني لو البكتيريا في الأصل الجليكوز
+
+714
+00:53:47,280 --> 00:53:49,360
+fermentation لكن ماعندها القدرة انها تعمل
+
+715
+00:53:49,360 --> 00:53:53,260
+fermentation لا اللاكتوز ولا السكراز أكيد هتستهلك
+
+716
+00:53:53,260 --> 00:53:57,340
+البروتين البيبتون الموجود في الميديا هتعملهدى
+
+717
+00:53:57,340 --> 00:54:01,480
+amination ويتحول لون ال slant للون ده يبقى هذا
+
+718
+00:54:01,480 --> 00:54:07,760
+بالنسبة لل principle اللى شرحناه سابقا بالنسبة
+
+719
+00:54:07,760 --> 00:54:10,920
+للخطوات قولنا بنعمل زراع على ال triple sugar iron
+
+720
+00:54:10,920 --> 00:54:14,120
+agaric slant من خلال ان انا بعمل stabbing لالبط و
+
+721
+00:54:14,120 --> 00:54:17,880
+ال slant بزرعه زيك زاك بعمل incubation لمدة 24
+
+722
+00:54:17,880 --> 00:54:23,330
+hours ممنوع قبل اطلع ممنوع بعدقلنا إن الـ timing
+
+723
+00:54:23,330 --> 00:54:27,690
+is critical في قراءة لـ triple sugar iron agar أو
+
+724
+00:54:27,690 --> 00:54:32,310
+لـ clagar iron agar لو أنا قرأت قبل 24 ساعة an
+
+725
+00:54:32,310 --> 00:54:36,650
+early reading could reveal yellow throughout the
+
+726
+00:54:36,650 --> 00:54:40,930
+media بقوللي إنه لو قرأت قبل 24 ساعة بيكون تم
+
+727
+00:54:40,930 --> 00:54:45,930
+استهلاك فقط الجليكوز صلوا fermentation هيتحول لون
+
+728
+00:54:45,930 --> 00:54:50,260
+الوسط للون yellowفبالتالي هذا هيصير في الـ Un
+
+729
+00:54:50,260 --> 00:54:53,240
+-aerobic condition و هيمتد اللون الأصفر في كل سيوب
+
+730
+00:54:53,240 --> 00:54:56,980
+فبالتالي كل الـ media هيكون لونها أصفر هيج أنا
+
+731
+00:54:56,980 --> 00:55:00,900
+leading one to conclude that the organism is a
+
+732
+00:55:00,900 --> 00:55:05,180
+lactose fermenter when it simply may not yet have
+
+733
+00:55:05,180 --> 00:55:09,820
+exercised the glycose بقوللي أنا هفكر أنه ال
+
+734
+00:55:09,820 --> 00:55:15,110
+organism صار عندهأو عمل fermentation للـ lactose
+
+735
+00:55:15,110 --> 00:55:20,550
+لكن هو فقط لحد الآن استهلك الجليكوز لو أنا قرأت
+
+736
+00:55:20,550 --> 00:55:25,610
+بعد 24 ساعة a reading after the لو أنا قرأت بعد 24
+
+737
+00:55:25,610 --> 00:55:28,910
+ساعة the lactose has been depleted بقولني أن ال
+
+738
+00:55:28,910 --> 00:55:34,220
+lactose بسله استهلاك كلهcould reveal a yellow pot
+
+739
+00:55:34,220 --> 00:55:37,620
+and red slant leading one to fossil conclude that
+
+740
+00:55:37,620 --> 00:55:40,740
+the organism is glucose only fermenter لو أنا قرأت
+
+741
+00:55:40,740 --> 00:55:45,240
+بعد 24 ساعات لكتاز والسكراز هيتم استهلاكهم
+
+742
+00:55:45,240 --> 00:55:48,120
+فبالتالي البكتيريا هتروح لمصادر تانية اللي هي ال
+
+743
+00:55:48,120 --> 00:55:51,560
+بيبتون فبالتالي هيصير في عندي deamination للبيبتون
+
+744
+00:55:51,560 --> 00:55:55,060
+في ال slant هيتحول لون ال slant للون red لكن في ال
+
+745
+00:55:55,060 --> 00:55:59,140
+pot هيكون yellow فبالتالي انا هفكر هذه النتيجة K
+
+746
+00:55:59,140 --> 00:56:04,450
+على A ان فقط البكتيرياعندها القدرة إنها تستعمل
+
+747
+00:56:04,450 --> 00:56:08,870
+fermentation للجليكوز وهي non-lactose fermenter
+
+748
+00:56:08,870 --> 00:56:14,330
+فهك هيصير في عندي falsely result بالنسبة للنتائج
+
+749
+00:56:14,330 --> 00:56:20,650
+لو اتكون فيه البطلون yellow في ال slant يلو يبقى
+
+750
+00:56:20,650 --> 00:56:24,430
+هك بعرف إن البكتيريا عملت gelicose و lactose
+
+751
+00:56:24,430 --> 00:56:29,360
+وsaccharose fermentationأتكون عندي acid هذا ال
+
+752
+00:56:29,360 --> 00:56:34,380
+acid اتحول لون ال وسط للون أصفر نتيجة وجود الفينال
+
+753
+00:56:34,380 --> 00:56:38,000
+red انتشر في ال slant وفي ال butt برمز لقيله
+
+754
+00:56:38,000 --> 00:56:43,060
+بالرمز A على A تمام هذا بالنسبة لأول رمز هذا لو
+
+755
+00:56:43,060 --> 00:56:48,640
+قرأت النتائج عند 24 ساعة لأنه لو أنا قرأت هان عند
+
+756
+00:56:48,640 --> 00:56:52,480
+أول 12 ساعة معناه أن فقط صار في عندي استهلاك
+
+757
+00:56:52,480 --> 00:56:57,340
+للجليكوز هذا كله بتتكلم عند 24 ساعةطيب لو لاحظت في
+
+758
+00:56:57,340 --> 00:57:00,580
+ال bot في عندي yellow لكن في ال slant في عندي red
+
+759
+00:57:00,580 --> 00:57:03,820
+في ال في ال yellow bot معناه انه صار في عندي فقط
+
+760
+00:57:03,820 --> 00:57:07,560
+gelatinous fermentation اتكون في عندي acid في ال
+
+761
+00:57:07,560 --> 00:57:10,820
+slant اتكون red يبقى صار في عندي deamination
+
+762
+00:57:10,820 --> 00:57:15,340
+لبيبتون بشكل aerobically بشكل في ظروف ال aerobic
+
+763
+00:57:15,340 --> 00:57:21,080
+condition تمام الرمز بما انه yellow بنحط A بما انه
+
+764
+00:57:21,080 --> 00:57:26,070
+hundred يبقى بنحط KRed على Red يبقى ما أصار في
+
+765
+00:57:26,070 --> 00:57:29,570
+عندي fermentation حتى ما أشوف لون Red في ال بط أو
+
+766
+00:57:29,570 --> 00:57:33,030
+في الإسلان يبقى ما أصار في عندي ماشوفة لون ال
+
+767
+00:57:33,030 --> 00:57:38,150
+yellow يبقى ما أصار في عندي fermentation إيش معناه
+
+768
+00:57:38,150 --> 00:57:40,430
+في ال Red في الإسلان معناه إنه صار في عندي
+
+769
+00:57:40,430 --> 00:57:44,310
+deamination للبيبتون في الظروف الهوائية في ال
+
+770
+00:57:44,310 --> 00:57:49,370
+European conditionالبكتيريا ماقتفت بحاجتها في
+
+771
+00:57:49,370 --> 00:57:52,810
+المواد الغذائية فعملت deamination للبيبتون بشكل
+
+772
+00:57:52,810 --> 00:57:56,890
+اضطراري في الظروف الـ anaerobic condition يبقى صار
+
+773
+00:57:56,890 --> 00:58:00,010
+في عندي بيبتون deamination aerobically and
+
+774
+00:58:00,010 --> 00:58:02,790
+anaerobically اتكون في عندي alkaline product
+
+775
+00:58:02,790 --> 00:58:07,410
+فاتحول اللونالميديا للون أحمر نتيجة تكون
+
+776
+00:58:07,410 --> 00:58:12,130
+الألقالييني برودك تبقى الرمز K على K هذه البكتيريا
+
+777
+00:58:12,130 --> 00:58:15,430
+بما أنه في الأصل هي non fermentation يبقى هي مش من
+
+778
+00:58:15,430 --> 00:58:19,290
+ال انتيرو بكترياسي لأن الانتيرو بكترياسي هي تعمل
+
+779
+00:58:19,290 --> 00:58:23,810
+جليكوز fermentation كلها لو ال red كان slant لكن
+
+780
+00:58:23,810 --> 00:58:27,370
+في البط ماصار في عندي أي تغير يبقى هان بحكي أنه
+
+781
+00:58:27,370 --> 00:58:30,530
+ماصار في عندي fermentation لكن صار في عنديهذه
+
+782
+00:58:30,530 --> 00:58:34,190
+اميناشن للبيبتون لكن فقط في الـ aerobic condition
+
+783
+00:58:34,190 --> 00:58:37,670
+تكون عندي alkaline product تتحول لون ال media أو
+
+784
+00:58:37,670 --> 00:58:41,670
+لون ال slant فقط للون أحمر بما أن مصر في عندي
+
+785
+00:58:41,670 --> 00:58:44,030
+fermentation يبقى هي مش من ال interior bacteria
+
+786
+00:58:44,030 --> 00:58:48,410
+سيقولنا برمز لها بالرمز K على no exchange لو ما
+
+787
+00:58:48,410 --> 00:58:53,510
+اتغير في عندي ال في ال slant أو في البطمعناه إما
+
+788
+00:58:53,510 --> 00:58:59,050
+أن البكتيريا بتنمو بشكل بطيء أو بالاصل أنا ما زرعت
+
+789
+00:58:59,050 --> 00:59:01,970
+بكتيريا هي بالاصل مش من عائلة الـ Enterobacterias
+
+790
+00:59:01,970 --> 00:59:06,990
+لأن أنا طبعا ال بكتيريا كل ال Enterobacterias
+
+791
+00:59:06,990 --> 00:59:10,950
+بتنمو عادي عند 24 ساعة بتكون نامية فبالتالي not
+
+792
+00:59:10,950 --> 00:59:13,750
+change على not change بحكي أنه not from
+
+793
+00:59:13,750 --> 00:59:17,250
+Enterobacterias قلنا لـ Black precipitates برمز
+
+794
+00:59:17,250 --> 00:59:23,030
+لإله بالرمز H2Sالـ Sulphur Reduction أو اللي هو
+
+795
+00:59:23,030 --> 00:59:25,430
+لما يصير في ندي الـ Reduction لالـ Sulphur قولنا
+
+796
+00:59:25,430 --> 00:59:28,310
+هذا بيصير في الـ Acidic Condition الناتج من ال
+
+797
+00:59:28,310 --> 00:59:31,670
+fermentation للجليكوز أو اللاكتوز أو السكاروز
+
+798
+00:59:31,670 --> 00:59:35,590
+فبالتالي لو تكون اللون الأسود لازم أكتب جنبها على
+
+799
+00:59:35,590 --> 00:59:41,170
+طول Aاللي هي في الـ Bot بحط جنبها A ممنوع أحط K
+
+800
+00:59:41,170 --> 00:59:45,410
+لأن الـ H2S بال reduction بيسوير في ظروف acidic
+
+801
+00:59:45,410 --> 00:59:50,110
+condition يبقى ممنوع أشوف نتيجة K على K مع الـ H2S
+
+802
+00:59:50,110 --> 00:59:53,750
+لأن الـ H2S بيسير في ظروف acidic condition يبقى من
+
+803
+00:59:53,750 --> 00:59:59,760
+حتى ما أشوف الـ H2S بحط في ال Bot Aلو اتكون في
+
+804
+00:59:59,760 --> 01:00:03,560
+عندي تشققات في ال media بحط بعرف انه والله ناتج
+
+805
+01:00:03,560 --> 01:00:08,120
+عملية ال fermentation acid مع gas فبضيف رمز اللي
+
+806
+01:00:08,120 --> 01:00:14,020
+هو ال gas جنب اللي هي الرمز A على A مثلا او K على
+
+807
+01:00:14,020 --> 01:00:18,180
+A بحط رمز جنب الرمز اللي هو ال gas معناه انه صار
+
+808
+01:00:18,180 --> 01:00:21,720
+في عندي من نواتج عملية ال fermentation كمان acid
+
+809
+01:00:22,270 --> 01:00:28,310
+هذا بالنسبة لكل الأشياء المحتمل انها تكون اول
+
+810
+01:00:28,310 --> 01:00:31,790
+رمزان هذا الرمز وهذا الرمز معناه انه هي من عائلة
+
+811
+01:00:31,790 --> 01:00:35,850
+الانتيرو بكترياسي لكن هذول التلاتة مش من عائلة
+
+812
+01:00:35,850 --> 01:00:38,510
+الانتيرو بكترياسي هذول التنين من عائلة الانتيرو
+
+813
+01:00:38,510 --> 01:00:42,270
+بكترياسي هي بتكون lactose fermenter هي بتكون non
+
+814
+01:00:42,270 --> 01:00:45,550
+lactose fermenter زي البروتيا السلمونيلا الشيجيلة
+
+815
+01:00:45,550 --> 01:00:49,710
+لكن هان بتكون من lactose fermenter الكوليفارم
+
+816
+01:00:50,090 --> 01:00:54,650
+التلاتة هدول مش من عائلة ال انتيرو بكترياسي طب
+
+817
+01:00:54,650 --> 01:01:01,690
+نيجي للنتائج أول واحدة بقولي ان هي رمزها K على A
+
+818
+01:01:01,690 --> 01:01:05,530
+تمام عندي لون اتكون لون Yellow و اتكون لون Red
+
+819
+01:01:05,530 --> 01:01:09,010
+يبقى هي K على A يبقى هي من عائلة ال انتيرو
+
+820
+01:01:09,010 --> 01:01:12,030
+بكترياسي اللي هي non-lactose fermenter زي
+
+821
+01:01:12,030 --> 01:01:16,050
+المارجانيلا مارجاني تمام ما اتكون في عندي جاز يبقى
+
+822
+01:01:16,050 --> 01:01:21,420
+خلاص K على A هاي البكتيريا Kعلى no change إذا
+
+823
+01:01:21,420 --> 01:01:25,780
+ملاحظين لون ال media تحت نفس هاي هاي ك control هاي
+
+824
+01:01:25,780 --> 01:01:28,760
+مش مزروعة ك control لون ال media هيك بيكون جاي على
+
+825
+01:01:28,760 --> 01:01:32,600
+أورنج فبنلاحظ إنه في ال slant اتكون لون red لكن في
+
+826
+01:01:32,600 --> 01:01:37,040
+البطك كان نفس اللون هذا ما تغير no change يبقى هي
+
+827
+01:01:37,040 --> 01:01:40,700
+صدمانة سقير جنازة يبقى هذا الرمز هي مش من عيلة ال
+
+828
+01:01:40,700 --> 01:01:43,640
+interior bacteriasia وفعلا الصدمانة سقير جنازة مش
+
+829
+01:01:43,640 --> 01:01:46,900
+من عيلة ال interior bacteriasia هاي مش مزروعة ك
+
+830
+01:01:46,900 --> 01:01:51,910
+controlهذا يمكن أن يتكون لون H2S production قلنا
+
+831
+01:01:51,910 --> 01:01:56,810
+من حد ما أشوف الـ H2S بحمل جانبه A لأن الـ H2S
+
+832
+01:01:56,810 --> 01:01:59,970
+production يحدث في ظروف acid condition يبقى أكيد
+
+833
+01:01:59,970 --> 01:02:05,210
+يكون هناك سارج ليكوز fermentation فوق اتكوين اللون
+
+834
+01:02:05,210 --> 01:02:10,010
+الريد يبقى بحكي K على A مع الـ H2S K على A يبقى هي
+
+835
+01:02:10,010 --> 01:02:14,130
+من عائلة الـ Enterobacteraceae من عائلة الـ non
+
+836
+01:02:14,130 --> 01:02:18,050
+-lactose fermenter لأنها K على AK على A بقى هي non
+
+837
+01:02:18,050 --> 01:02:20,770
+-lactose fermentation اتكون عندي H2S production
+
+838
+01:02:20,770 --> 01:02:27,150
+يبقى احتمال كبير انها تكون هي البروتياز بأنه اتكون
+
+839
+01:02:27,150 --> 01:02:32,070
+عندي ال H2S production يبقى هي البروتياز لكن في
+
+840
+01:02:32,070 --> 01:02:36,130
+عندي برضه كمان السلمونيلا بفرق بينهم بفحص اليورياز
+
+841
+01:02:36,920 --> 01:02:39,540
+Equally فيين دي صار فيين دي هان تشقيقات إذا
+
+842
+01:02:39,540 --> 01:02:44,500
+ملاحظين في ال media يبقى بحط رمز ال gas تمام و بما
+
+843
+01:02:44,500 --> 01:02:47,800
+إنه في ال slant و في ال path تتكون لون أصفر و أصفر
+
+844
+01:02:47,800 --> 01:02:52,460
+يبقى a على a with gas يبقى ها هي النتيجة بنقولنا
+
+845
+01:02:52,460 --> 01:02:56,280
+ال gas و ال H2S بضيفها جنب الرمز K على A أو على A
+
+846
+01:02:56,280 --> 01:03:03,900
+على M هذا بالنسبة للنتائج نيجي لهدول هان الرمز
+
+847
+01:03:03,900 --> 01:03:09,340
+اللي هو Kعلى A بما أنه عندي أتكون H2S يبقى اللي
+
+848
+01:03:09,340 --> 01:03:15,720
+تحتها بتكون A يبقى K على A with H2S هان K على A
+
+849
+01:03:15,720 --> 01:03:25,800
+with H2S with gas كمان مع gas نكتب K
+
+850
+01:03:25,800 --> 01:03:30,650
+بما أنه لونه أحمر فوق على Aطبعا لأنه في اندي H2S
+
+851
+01:03:30,650 --> 01:03:33,510
+production فعلى طول بعرف أنه اللي تحت اللون
+
+852
+01:03:33,510 --> 01:03:41,950
+المفترض يكون أصفر اللي هو A fermentation مع
+
+853
+01:03:41,950 --> 01:03:48,830
+H2S
+
+854
+01:03:48,830 --> 01:03:51,490
+هذا بالنسبة لأول واحدة
+
+855
+01:03:58,590 --> 01:04:03,990
+النتيجة التانية هي نفسها لكن مع اتكون في عندي gas
+
+856
+01:04:15,630 --> 01:04:19,870
+يبقى بما أنه في عندي اللون الأسود يبقى H2S يتكون
+
+857
+01:04:19,870 --> 01:04:23,890
+في عندي فيه تشققات يبقى في عندي gas طبعا بالنسبة
+
+858
+01:04:23,890 --> 01:04:28,090
+للبط بما أنه أسود اللون H2S يبقى على طول بدي أعرف
+
+859
+01:04:28,090 --> 01:04:31,170
+ايه ممنوع أحط K في ال triple sugar iron agar ممنوع
+
+860
+01:04:31,170 --> 01:04:35,070
+يكون في عندي هن H2S و أحط K فقط ايه لأن ال
+
+861
+01:04:35,070 --> 01:04:38,450
+production H2S بيحصل في ظروف acid condition فوق
+
+862
+01:04:38,450 --> 01:04:45,070
+تكون اللون الأحمر يبقى K هن K اللون الأحمر فوق على
+
+863
+01:04:49,250 --> 01:04:57,250
+هنا تلاحظ ان كتكون
+
+864
+01:04:57,250 --> 01:05:03,030
+اللون الأحمر فتحت
+
+865
+01:05:03,030 --> 01:05:09,330
+كتكون
+
+866
+01:05:09,330 --> 01:05:14,130
+اللون الأحمر كعلى ك
+
+867
+01:05:19,880 --> 01:05:24,220
+مافي عندي اي تشققات ومافي عندي اي طبعا H2O
+
+868
+01:05:24,220 --> 01:05:28,560
+production يبقى high مش من عقل السلينتيرو بكترياسي
+
+869
+01:05:28,560 --> 01:05:33,180
+هان اتكون عندي A على
+
+870
+01:05:33,180 --> 01:05:38,420
+A اذا ملاحظين لون أصفر طبعا فوق لون أصفر تحت يبقى
+
+871
+01:05:38,420 --> 01:05:41,420
+A على A مافي عندي اي تشققات مافي عندي لون أسود
+
+872
+01:05:41,420 --> 01:05:46,700
+يبقى فقط A على A هان K فوق اتكون اللون الأحمر على
+
+873
+01:05:46,700 --> 01:05:52,870
+تحت في عندي اللون الأصفر Aمع وجود اللي هو الـ gas
+
+874
+01:05:52,870 --> 01:05:56,190
+في عندي هان تشققات يبقى في عندي gas يبقى K على A
+
+875
+01:05:56,190 --> 01:05:59,290
+with gas يبقى هاي من عقلة الـ enterobacteriasi هان
+
+876
+01:05:59,290 --> 01:06:03,610
+no change على no change يعملها بالاصل ك control
+
+877
+01:06:03,610 --> 01:06:10,010
+هاي
+
+878
+01:06:10,010 --> 01:06:12,550
+يبقى مش من عقلة الـ enterobacteriasi يبقى هاي مش
+
+879
+01:06:12,550 --> 01:06:15,750
+من عقلة الـ enterobacteriasiهي مش من عائلة ال
+
+880
+01:06:15,750 --> 01:06:18,650
+انتيرو بكترياسي لكن هي من عائلة ال انتيرو بكترياسي
+
+881
+01:06:18,650 --> 01:06:21,650
+هي من عائلة ال انتيرو بكترياسي هي من عائلة ال
+
+882
+01:06:21,650 --> 01:06:24,370
+انتيرو بكترياسي وهي كذلك هي و هي من عائلة ال
+
+883
+01:06:24,370 --> 01:06:26,890
+انتيرو بكترياسي يبقى عائلة ال انتيرو بكترياسي اما
+
+884
+01:06:26,890 --> 01:06:31,470
+تكون a على a او k على a لكن لو اتكون k على k او k
+
+885
+01:06:31,470 --> 01:06:33,950
+على not change او not change على not change يبقى
+
+886
+01:06:33,950 --> 01:06:37,790
+هي مش من عائلة ال انتيرو بكترياسي لو كانت a على a
+
+887
+01:06:37,790 --> 01:06:41,550
+يبقى هي من ال coliform lactose fermenter لكن لو
+
+888
+01:06:41,550 --> 01:06:45,470
+كانت k على a يبقى هيمن الـ Non-Lactose Fermenter
+
+889
+01:06:45,470 --> 01:06:51,050
+اللي هي من الـ Non-Colliefer هذا بالنسبة للـ S
+
+890
+01:06:51,050 --> 01:06:54,850
+اللي هو Triple Sugar Iron Agar هكذا نكون خلصنا أول
+
+891
+01:06:54,850 --> 01:06:58,890
+ميديتين الـ SIM و Triple Sugar Iron Agar أو
+
+892
+01:06:58,890 --> 01:07:02,810
+Trigger Sugar Iron Agar
+
diff --git a/PL9fwy3NUQKwZWgAb_V1ATlRRsH-6i3cPC/owBu12fuBog_raw.json b/PL9fwy3NUQKwZWgAb_V1ATlRRsH-6i3cPC/owBu12fuBog_raw.json
new file mode 100644
index 0000000000000000000000000000000000000000..b20c6cdd6cb3ef5c9d529fa3be31fb0adf898936
--- /dev/null
+++ b/PL9fwy3NUQKwZWgAb_V1ATlRRsH-6i3cPC/owBu12fuBog_raw.json
@@ -0,0 +1 @@
+{"segments": [{"id": 1, "seek": 2733, "start": 3.17, "end": 27.33, "text": "السلام عليكم ورحمة الله وبركاته يعطيكم العافية جميعا نستكمل معكم سلسلة محاضرات مساق الـ Microbiology العملي في هذا اللقاء هتكون المحاضرة بعنوان Single Media Multiple Test في المعامل السابقة كنا نستخدم ميديا بتكشف على فحص كيميائي واحد one biochemical test", "tokens": [6027, 3794, 37440, 25894, 24793, 4032, 2288, 35571, 3660, 21984, 4032, 26890, 4117, 9307, 3224, 37495, 9566, 1829, 24793, 18863, 31845, 10632, 10874, 2304, 40228, 995, 8717, 14851, 24793, 1211, 20449, 24793, 8608, 1211, 3794, 37977, 3714, 5016, 46958, 2288, 9307, 47524, 995, 4587, 2423, 39184, 5818, 20027, 1793, 18863, 2304, 20292, 8978, 23758, 13672, 4587, 16606, 8032, 2655, 30544, 9673, 5016, 46958, 25720, 45030, 1863, 2407, 7649, 31248, 14741, 40056, 9279, 8978, 9673, 3615, 10943, 1211, 21136, 16758, 28671, 9122, 8315, 8717, 14851, 9778, 40448, 3714, 1829, 16254, 995, 39894, 4117, 8592, 5172, 15844, 6156, 5016, 9381, 9122, 32640, 1829, 16373, 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"end": 71.72, "word": " ميديا", "probability": 0.9886474609375}, {"start": 71.72, "end": 72.24, "word": " من", "probability": 0.98828125}, {"start": 72.24, "end": 72.54, "word": " هاي", "probability": 0.75}, {"start": 72.54, "end": 73.16, "word": " الميديات", "probability": 0.9580078125}, {"start": 73.16, "end": 73.9, "word": " بالتفصيل", "probability": 0.9779296875}], "temperature": 1.0}, {"id": 4, "seek": 9847, "start": 74.23, "end": 98.47, "text": "شو الفحوصات اللى بتكشف عنها كل ميديا كيف أنا بقدر أفسر نتائج هذه الميديا خلينا نبدأ بأول ميديا اللى هى الـ SIM media قولنا إن اسم الميديا جاى من أسماء الفحوصات اللى أنا بقدر أعملها في هذه الميديا بالنسبة لـ SIM media الـ S يرمز لـ sulphide", "tokens": [8592, 2407, 27188, 5016, 2407, 9381, 9307, 13672, 7578, 39894, 4117, 8592, 5172, 18871, 11296, 28242, 3714, 1829, 16254, 995, 9122, 33911, 41850, 4724, 28543, 2288, 5551, 36178, 2288, 8717, 2655, 16373, 7435, 29538, 9673, 1829, 16254, 995, 16490, 1211, 9957, 995, 8717, 44510, 10721, 4724, 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البروتياس التانتان هدول H2S positive احنا قلنا اذا بتتذكروا بفحص البروتياس بفحص اليورياز sorry بفحص اليورياز قلنا بيفرق بين ال non-lactose fermenter اللي هم السلمونيلا شجلة البروتياس هدولة التلاتة يعتبروا non-lactose fermenter", "tokens": [27842, 2423, 39184, 10399, 299, 1605, 13672, 1829, 39896, 6225, 16373, 37977, 2423, 39184, 10399, 996, 14125, 72, 617, 8346, 21136, 19528, 11536, 1829, 15040, 4032, 2423, 26890, 2407, 31371, 32277, 16712, 7649, 2655, 7649, 8032, 3215, 12610, 389, 17, 50, 3353, 1975, 5016, 8315, 12174, 1211, 8315, 1975, 15730, 39894, 2655, 8848, 37983, 14407, 4724, 5172, 5016, 9381, 2423, 26890, 2407, 31371, 32277, 4724, 5172, 5016, 9381, 45595, 13063, 1829, 31377, 2597, 4724, 5172, 5016, 9381, 45595, 13063, 1829, 31377, 12174, 1211, 8315, 4724, 33911, 2288, 4587, 49374, 2423, 2107, 12, 75, 578, 541, 38300, 260, 13672, 1829, 8032, 2304, 21136, 19528, 11536, 1829, 15040, 13412, 7435, 37977, 2423, 26890, 2407, 31371, 32277, 8032, 3215, 12610, 3660, 16712, 1211, 9307, 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1863, 35457, 3660, 24976, 29399, 16373, 7435, 39896, 2423, 9381, 13063, 2655, 7649, 8717, 36178, 25124, 1829, 38207], "avg_logprob": -0.24367559523809523, "compression_ratio": 1.0454545454545454, "no_speech_prob": 0.0, "words": [{"start": 1290.41, "end": 1291.05, "word": "بالنسبة", "probability": 0.96416015625}, {"start": 1291.05, "end": 1292.69, "word": " للنتائج", "probability": 0.8668212890625}, {"start": 1292.69, "end": 1293.13, "word": " هي", "probability": 0.40380859375}, {"start": 1293.13, "end": 1293.89, "word": " الصورتان", "probability": 0.658984375}, {"start": 1293.89, "end": 1294.25, "word": " نفس", "probability": 0.994873046875}, {"start": 1294.25, "end": 1294.99, "word": " الشيء", "probability": 0.8151041666666666}], "temperature": 1.0}, {"id": 55, "seek": 132362, "start": 1295.48, "end": 1323.62, "text": "أول شئ هنقرأ فحص الـ Motility أكيد اللي بالنسبة لـ Motility هي الـ bacteria موطة اللي بنلاحظ أنه في عندي بعيدا عن خط الزراعة في عندي turbidity بعدها هقرأ الـ edge to us هي negative edge to us negative فحص ال Indol دفت الكوفاكس رياجانت ماتحول اللون للون أحمر في أعلى الحلقة يبقى هي في أعلى ال tube يبقى هي Indol negative", "tokens": [10721, 12610, 13412, 19986, 8032, 1863, 4587, 2288, 10721, 6156, 5016, 9381, 2423, 39184, 8956, 1140, 5551, 4117, 25708, 13672, 1829, 20666, 1863, 35457, 3660, 5296, 39184, 8956, 1140, 39896, 2423, 39184, 11763, 3714, 2407, 9566, 3660, 13672, 1829, 44945, 15040, 5016, 19913, 14739, 3224, 8978, 18871, 16254, 45030, 25708, 995, 18871, 16490, 9566, 6024, 110, 23557, 27884, 8978, 18871, 16254, 18252, 17711, 39182, 11296, 8032, 4587, 2288, 10721, 2423, 39184, 4691, 281, 505, 39896, 3671, 4691, 281, 505, 3671, 6156, 5016, 9381, 2423, 2333, 401, 11778, 5172, 2655, 33251, 38688, 995, 4117, 3794, 12602, 1829, 26108, 7649, 2655, 3714, 9307, 5016, 12610, 13672, 11536, 5296, 1211, 11536, 5551, 5016, 29973, 8978, 5551, 3615, 23942, 21542, 1211, 28671, 7251, 3555, 4587, 7578, 39896, 8978, 5551, 3615, 23942, 2423, 9917, 7251, 3555, 4587, 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"probability": 0.7115478515625}, {"start": 2048.16, "end": 2048.68, "word": " نرمز", "probability": 0.8240966796875}, {"start": 2048.68, "end": 2049.38, "word": " النتيجة", "probability": 0.96044921875}, {"start": 2049.38, "end": 2049.64, "word": " K", "probability": 0.88623046875}, {"start": 2049.64, "end": 2050.0, "word": " على", "probability": 0.91015625}, {"start": 2050.0, "end": 2050.34, "word": " A", "probability": 0.91064453125}], "temperature": 1.0}, {"id": 84, "seek": 207523, "start": 2051.07, "end": 2075.23, "text": "هذا بالنسبة لو البكتيريا هيك قرأت او هيك الفحص هيك النتيجة بتكون لو البكتيريا جيليكوز fermentation لكن non-lactose fermenter بتكون red على yellow لكن لو كانت جيليكوز و lactose fermentation بتكون yellow على yellow ا على ا هذا بعد 24 ساعة لكن خلال أول 12 ساعة لو كانت lactose", "tokens": [3224, 15730, 20666, 1863, 35457, 3660, 45164, 29739, 4117, 2655, 13546, 25528, 39896, 4117, 12174, 2288, 10721, 2655, 1975, 2407, 39896, 4117, 27188, 5016, 9381, 39896, 4117, 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2059.91, "word": " لكن", "probability": 0.91455078125}, {"start": 2059.91, "end": 2060.25, "word": " non", "probability": 0.5263671875}, {"start": 2060.25, "end": 2060.97, "word": "-lactose", "probability": 0.8935546875}, {"start": 2060.97, "end": 2061.59, "word": " fermenter", "probability": 0.60107421875}, {"start": 2061.59, "end": 2062.01, "word": " بتكون", "probability": 0.94287109375}, {"start": 2062.01, "end": 2062.35, "word": " red", "probability": 0.54248046875}, {"start": 2062.35, "end": 2062.63, "word": " على", "probability": 0.830078125}, {"start": 2062.63, "end": 2063.09, "word": " yellow", "probability": 0.80029296875}, {"start": 2063.09, "end": 2063.81, "word": " لكن", "probability": 0.8720703125}, {"start": 2063.81, "end": 2063.99, "word": " لو", "probability": 0.9873046875}, {"start": 2063.99, "end": 2064.39, "word": " كانت", "probability": 0.988037109375}, {"start": 2064.39, "end": 2065.09, "word": " جيليكوز", "probability": 0.9702962239583334}, {"start": 2065.09, 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"word": " 24", "probability": 0.85009765625}, {"start": 2070.49, "end": 2071.25, "word": " ساعة", "probability": 0.9466145833333334}, {"start": 2071.25, "end": 2071.85, "word": " لكن", "probability": 0.91650390625}, {"start": 2071.85, "end": 2072.49, "word": " خلال", "probability": 0.994140625}, {"start": 2072.49, "end": 2072.91, "word": " أول", "probability": 0.92724609375}, {"start": 2072.91, "end": 2073.33, "word": " 12", "probability": 0.8984375}, {"start": 2073.33, "end": 2073.85, "word": " ساعة", "probability": 0.92236328125}, {"start": 2073.85, "end": 2074.09, "word": " لو", "probability": 0.9892578125}, {"start": 2074.09, "end": 2074.57, "word": " كانت", "probability": 0.97998046875}, {"start": 2074.57, "end": 2075.23, "word": " lactose", "probability": 0.978515625}], "temperature": 1.0}, {"id": 85, "seek": 209685, "start": 2076.13, "end": 2096.85, "text": "أو non-lactose fermenter ستكون A على A عشان هي كم نقرأ النتيجة عند 24 ساعة هدنا هنتكلم في هذا الموضوع بالنسبة لو البكتيريا كانت بالاصل non-gelicose fermenter هذا اتكلمنا عنه", "tokens": [10721, 2407, 2107, 12, 75, 578, 541, 38300, 260, 8608, 2655, 30544, 316, 15844, 316, 6225, 8592, 7649, 39896, 9122, 2304, 8717, 4587, 2288, 10721, 28239, 31371, 7435, 3660, 43242, 4022, 8608, 995, 27884, 8032, 3215, 8315, 8032, 29399, 4117, 19528, 8978, 23758, 9673, 2407, 11242, 45367, 20666, 1863, 35457, 3660, 45164, 29739, 4117, 2655, 13546, 25528, 25961, 2655, 20666, 33546, 1211, 2107, 12, 10345, 299, 541, 38300, 260, 23758, 1975, 2655, 4117, 19528, 8315, 18871, 3224], "avg_logprob": -0.27043268237358487, "compression_ratio": 1.4702702702702704, "no_speech_prob": 1.3232231140136719e-05, "words": [{"start": 2076.13, "end": 2076.67, "word": "أو", "probability": 0.56744384765625}, {"start": 2076.67, "end": 2078.99, "word": " non", "probability": 0.17724609375}, {"start": 2078.99, "end": 2079.77, "word": "-lactose", "probability": 0.84130859375}, {"start": 2079.77, "end": 2082.47, "word": " fermenter", "probability": 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0.8590494791666666}, {"start": 2087.55, "end": 2088.13, "word": " هدنا", "probability": 0.4881998697916667}, {"start": 2088.13, "end": 2088.69, "word": " هنتكلم", "probability": 0.987060546875}, {"start": 2088.69, "end": 2089.11, "word": " في", "probability": 0.90576171875}, {"start": 2089.11, "end": 2089.45, "word": " هذا", "probability": 0.93115234375}, {"start": 2089.45, "end": 2090.31, "word": " الموضوع", "probability": 0.9986572265625}, {"start": 2090.31, "end": 2091.77, "word": " بالنسبة", "probability": 0.8260498046875}, {"start": 2091.77, "end": 2092.11, "word": " لو", "probability": 0.81689453125}, {"start": 2092.11, "end": 2093.01, "word": " البكتيريا", "probability": 0.9626953125}, {"start": 2093.01, "end": 2093.47, "word": " كانت", "probability": 0.986572265625}, {"start": 2093.47, "end": 2094.13, "word": " بالاصل", "probability": 0.91796875}, {"start": 2094.13, "end": 2094.45, "word": " non", "probability": 0.76318359375}, {"start": 2094.45, "end": 2095.19, "word": "-gelicose", "probability": 0.79986572265625}, {"start": 2095.19, "end": 2095.73, "word": " fermenter", "probability": 0.9365234375}, {"start": 2095.73, "end": 2095.91, "word": " هذا", "probability": 0.54931640625}, {"start": 2095.91, "end": 2096.49, "word": " اتكلمنا", "probability": 0.883935546875}, {"start": 2096.49, "end": 2096.85, "word": " عنه", "probability": 0.979248046875}], "temperature": 1.0}, {"id": 86, "seek": 212338, "start": 2097.38, "end": 2123.38, "text": "هذا كله لو كانت البكتيريا في الأصل هي Gelicose Fermenter لكن لو البكتيريا هي ماعندها القدرة انها تعمل fermentation لأبسط سكر الجليكوز يبقى أكيد مش هتعمل fermentation للسكر الثنائي اللكتاز والسكراز فبالتالي هتروح لمين هتروح للبيبتون هتعمله deamination هيتكون عندي alkaline product هيتحول لون ال media للون red", "tokens": [3224, 15730, 28242, 3224, 45164, 25961, 2655, 29739, 4117, 2655, 13546, 25528, 8978, 16247, 36520, 39896, 16142, 299, 541, 10728, 518, 260, 44381, 45164, 29739, 4117, 2655, 13546, 25528, 39896, 19446, 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"probability": 0.85888671875}, {"start": 2098.64, "end": 2099.0, "word": " كانت", "probability": 0.967041015625}, {"start": 2099.0, "end": 2099.5, "word": " البكتيريا", "probability": 0.96396484375}, {"start": 2099.5, "end": 2099.64, "word": " في", "probability": 0.916015625}, {"start": 2099.64, "end": 2100.0, "word": " الأصل", "probability": 0.837646484375}, {"start": 2100.0, "end": 2100.26, "word": " هي", "probability": 0.77978515625}, {"start": 2100.26, "end": 2100.94, "word": " Gelicose", "probability": 0.5567220052083334}, {"start": 2100.94, "end": 2101.68, "word": " Fermenter", "probability": 0.5708821614583334}, {"start": 2101.68, "end": 2102.26, "word": " لكن", "probability": 0.7587890625}, {"start": 2102.26, "end": 2102.48, "word": " لو", "probability": 0.919921875}, {"start": 2102.48, "end": 2103.08, "word": " البكتيريا", "probability": 0.97646484375}, {"start": 2103.08, "end": 2103.42, "word": " هي", "probability": 0.76025390625}, {"start": 2103.42, "end": 2104.02, "word": " ماعندها", "probability": 0.723388671875}, {"start": 2104.02, "end": 2104.32, "word": " القدرة", "probability": 0.9601236979166666}, {"start": 2104.32, "end": 2104.54, "word": " انها", "probability": 0.525146484375}, {"start": 2104.54, "end": 2104.9, "word": " تعمل", "probability": 0.9803059895833334}, {"start": 2104.9, "end": 2105.44, "word": " fermentation", "probability": 0.9091796875}, {"start": 2105.44, "end": 2106.36, "word": " لأبسط", "probability": 0.96171875}, {"start": 2106.36, "end": 2106.78, "word": " سكر", "probability": 0.873291015625}, {"start": 2106.78, "end": 2107.5, "word": " الجليكوز", "probability": 0.8552734375}, {"start": 2107.5, "end": 2107.74, "word": " يبقى", "probability": 0.9581298828125}, {"start": 2107.74, "end": 2108.14, "word": " أكيد", "probability": 0.9114583333333334}, {"start": 2108.14, "end": 2108.32, "word": " مش", "probability": 0.93798828125}, {"start": 2108.32, "end": 2108.72, "word": " هتعمل", "probability": 0.9864501953125}, {"start": 2108.72, "end": 2109.34, "word": " fermentation", "probability": 0.97265625}, {"start": 2109.34, "end": 2110.64, "word": " للسكر", "probability": 0.7706705729166666}, {"start": 2110.64, "end": 2111.3, "word": " الثنائي", "probability": 0.93154296875}, {"start": 2111.3, "end": 2112.02, "word": " اللكتاز", "probability": 0.73583984375}, {"start": 2112.02, "end": 2112.84, "word": " والسكراز", "probability": 0.6925048828125}, {"start": 2112.84, "end": 2114.02, "word": " فبالتالي", "probability": 0.9857584635416666}, {"start": 2114.02, "end": 2114.56, "word": " هتروح", "probability": 0.9754638671875}, {"start": 2114.56, "end": 2115.02, "word": " لمين", "probability": 0.9111328125}, {"start": 2115.02, "end": 2115.54, "word": " هتروح", "probability": 0.933837890625}, {"start": 2115.54, "end": 2116.3, "word": " للبيبتون", "probability": 0.739599609375}, {"start": 2116.3, "end": 2117.68, "word": " هتعمله", "probability": 0.9281005859375}, {"start": 2117.68, "end": 2118.86, "word": " deamination", "probability": 0.691650390625}, {"start": 2118.86, "end": 2119.56, "word": " هيتكون", "probability": 0.78271484375}, {"start": 2119.56, "end": 2119.88, "word": " عندي", "probability": 0.6993408203125}, {"start": 2119.88, "end": 2120.5, "word": " alkaline", "probability": 0.892333984375}, {"start": 2120.5, "end": 2120.98, "word": " product", "probability": 0.970703125}, {"start": 2120.98, "end": 2121.9, "word": " هيتحول", "probability": 0.9078369140625}, {"start": 2121.9, "end": 2122.14, "word": " لون", "probability": 0.98095703125}, {"start": 2122.14, "end": 2122.3, "word": " ال", "probability": 0.78466796875}, {"start": 2122.3, "end": 2122.56, "word": " media", "probability": 0.9384765625}, {"start": 2122.56, "end": 2123.08, "word": " للون", "probability": 0.9697265625}, {"start": 2123.08, "end": 2123.38, "word": " red", "probability": 0.63720703125}], "temperature": 1.0}, {"id": 87, "seek": 215253, "start": 2123.89, "end": 2152.53, "text": "هيصير اللون الـ Red قلنا الـ Deamination للبيبتون هيصير في الـ Slant في الـ Aerobic condition يبقى هيتحول اللون الـ Slant للون الـ Red لكن البط مش هيصله أي تغير No, it changed بقوللي ممكن برضه لو البكتيريا في الأصل non-gelicose fermenter ممكن إنها لو ما عندها القدرة إنها تعمل للـ Gelicose فبتعرفش هتعمل للاكتوز ولا للسكراز أكيد هتعمل Deamination للبيبتون في", "tokens": [3224, 1829, 9381, 13546, 13672, 11536, 2423, 39184, 4477, 12174, 1211, 8315, 2423, 39184, 1346, 335, 2486, 24976, 21292, 3555, 2655, 11536, 39896, 9381, 13546, 8978, 2423, 39184, 6187, 394, 8978, 2423, 39184, 32459, 22234, 4188, 7251, 3555, 4587, 7578, 8032, 36081, 5016, 12610, 13672, 11536, 2423, 39184, 6187, 394, 5296, 1211, 11536, 2423, 39184, 4477, 44381, 29739, 9566, 37893, 39896, 36520, 3224, 36632, 6055, 17082, 13546, 883, 11, 309, 3105, 4724, 39648, 20292, 3714, 43020, 4724, 43042, 3224, 45164, 29739, 4117, 2655, 13546, 25528, 8978, 16247, 36520, 2107, 12, 10345, 299, 541, 38300, 260, 3714, 43020, 36145, 11296, 45164, 19446, 43242, 11296, 25062, 3215, 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"probability": 0.6493326822916666}, {"start": 2126.43, "end": 2127.01, "word": " للبيبتون", "probability": 0.79111328125}, {"start": 2127.01, "end": 2127.55, "word": " هيصير", "probability": 0.7408854166666666}, {"start": 2127.55, "end": 2127.99, "word": " في", "probability": 0.93408203125}, {"start": 2127.99, "end": 2128.99, "word": " الـ", "probability": 0.5081787109375}, {"start": 2128.99, "end": 2129.49, "word": " Slant", "probability": 0.7076416015625}, {"start": 2129.49, "end": 2129.81, "word": " في", "probability": 0.84716796875}, {"start": 2129.81, "end": 2130.05, "word": " الـ", "probability": 0.747314453125}, {"start": 2130.05, "end": 2130.63, "word": " Aerobic", "probability": 0.68310546875}, {"start": 2130.63, "end": 2131.65, "word": " condition", "probability": 0.62255859375}, {"start": 2131.65, "end": 2132.19, "word": " يبقى", "probability": 0.946044921875}, {"start": 2132.19, "end": 2132.67, "word": " هيتحول", "probability": 0.9000244140625}, {"start": 2132.67, "end": 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"word": " No,", "probability": 0.129150390625}, {"start": 2137.35, "end": 2137.41, "word": " it", "probability": 0.85205078125}, {"start": 2137.41, "end": 2137.85, "word": " changed", "probability": 0.5400390625}, {"start": 2137.85, "end": 2139.13, "word": " بقوللي", "probability": 0.6529134114583334}, {"start": 2139.13, "end": 2139.47, "word": " ممكن", "probability": 0.963623046875}, {"start": 2139.47, "end": 2140.07, "word": " برضه", "probability": 0.9873046875}, {"start": 2140.07, "end": 2140.37, "word": " لو", "probability": 0.91650390625}, {"start": 2140.37, "end": 2140.91, "word": " البكتيريا", "probability": 0.94375}, {"start": 2140.91, "end": 2141.03, "word": " في", "probability": 0.92138671875}, {"start": 2141.03, "end": 2141.43, "word": " الأصل", "probability": 0.88525390625}, {"start": 2141.43, "end": 2141.77, "word": " non", "probability": 0.345947265625}, {"start": 2141.77, "end": 2142.53, "word": "-gelicose", "probability": 0.8529052734375}, {"start": 2142.53, "end": 2143.31, "word": " fermenter", "probability": 0.7685546875}, {"start": 2143.31, "end": 2143.71, "word": " ممكن", "probability": 0.972900390625}, {"start": 2143.71, "end": 2144.21, "word": " إنها", "probability": 0.781494140625}, {"start": 2144.21, "end": 2144.69, "word": " لو", "probability": 0.869140625}, {"start": 2144.69, "end": 2144.89, "word": " ما", "probability": 0.681640625}, {"start": 2144.89, "end": 2145.17, "word": " عندها", "probability": 0.94189453125}, {"start": 2145.17, "end": 2145.43, "word": " القدرة", "probability": 0.9775390625}, {"start": 2145.43, "end": 2145.61, "word": " إنها", "probability": 0.7191162109375}, {"start": 2145.61, "end": 2145.93, "word": " تعمل", "probability": 0.9386393229166666}, {"start": 2145.93, "end": 2146.11, "word": " للـ", "probability": 0.705810546875}, {"start": 2146.11, "end": 2146.51, "word": " Gelicose", "probability": 0.7874348958333334}, {"start": 2146.51, "end": 2146.99, "word": " فبتعرفش", "probability": 0.7085367838541666}, {"start": 2146.99, "end": 2147.39, "word": " هتعمل", "probability": 0.931884765625}, {"start": 2147.39, "end": 2147.93, "word": " للاكتوز", "probability": 0.8234049479166666}, {"start": 2147.93, "end": 2148.11, "word": " ولا", "probability": 0.89111328125}, {"start": 2148.11, "end": 2148.85, "word": " للسكراز", "probability": 0.6082763671875}, {"start": 2148.85, "end": 2149.75, "word": " أكيد", "probability": 0.9596354166666666}, {"start": 2149.75, "end": 2150.27, "word": " هتعمل", "probability": 0.986328125}, {"start": 2150.27, "end": 2151.07, "word": " Deamination", "probability": 0.8321940104166666}, {"start": 2151.07, "end": 2152.19, "word": " للبيبتون", "probability": 0.92822265625}, {"start": 2152.19, "end": 2152.53, "word": " في", "probability": 0.986328125}], "temperature": 1.0}, {"id": 88, "seek": 217990, "start": 2153.06, "end": 2179.9, "text": "الـ aerobic condition في الظروف الهوائية هيتحول لون الـ slant للون red طيب لو البكتيريا ما اكتفت بحاجتها من المواد الغذائية بقوللي هتعمل deamination للبيبتون في ظروف unaerobic condition بشكل اضطراري يبقى هان صار عند deamination للبيبتون في ظروف unaerobic condition لكن", "tokens": [6027, 39184, 11207, 22234, 4188, 8978, 6024, 116, 32887, 5172, 2423, 3224, 2407, 16373, 10632, 8032, 36081, 5016, 12610, 5296, 11536, 2423, 39184, 1061, 394, 5296, 1211, 11536, 2182, 23032, 1829, 3555, 45164, 29739, 4117, 2655, 13546, 25528, 19446, 1975, 4117, 2655, 5172, 2655, 4724, 5016, 26108, 2655, 11296, 9154, 9673, 14407, 3215, 6024, 118, 8848, 16373, 10632, 4724, 39648, 20292, 8032, 2655, 25957, 1211, 368, 335, 2486, 24976, 21292, 3555, 2655, 11536, 8978, 1357, 116, 32887, 5172, 517, 64, 260, 22234, 4188, 4724, 8592, 28820, 1975, 11242, 9566, 2288, 9640, 1829, 7251, 3555, 4587, 7578, 8032, 7649, 20328, 9640, 43242, 368, 335, 2486, 24976, 21292, 3555, 2655, 11536, 8978, 1357, 116, 32887, 5172, 2002, 260, 22234, 4188, 44381], "avg_logprob": -0.14921874664723872, "compression_ratio": 1.9209302325581394, "no_speech_prob": 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"word": " red", "probability": 0.2286376953125}, {"start": 2159.9, "end": 2160.68, "word": " طيب", "probability": 0.9609375}, {"start": 2160.68, "end": 2160.9, "word": " لو", "probability": 0.86865234375}, {"start": 2160.9, "end": 2161.52, "word": " البكتيريا", "probability": 0.9607421875}, {"start": 2161.52, "end": 2161.7, "word": " ما", "probability": 0.78173828125}, {"start": 2161.7, "end": 2162.26, "word": " اكتفت", "probability": 0.96357421875}, {"start": 2162.26, "end": 2163.68, "word": " بحاجتها", "probability": 0.9875}, {"start": 2163.68, "end": 2163.96, "word": " من", "probability": 0.99169921875}, {"start": 2163.96, "end": 2165.08, "word": " المواد", "probability": 0.92578125}, {"start": 2165.08, "end": 2165.7, "word": " الغذائية", "probability": 0.9880859375}, {"start": 2165.7, "end": 2166.22, "word": " بقوللي", "probability": 0.73828125}, {"start": 2166.22, "end": 2167.0, "word": " هتعمل", "probability": 0.9921875}, {"start": 2167.0, "end": 2167.86, "word": " deamination", "probability": 0.7392578125}, {"start": 2167.86, "end": 2168.66, "word": " للبيبتون", "probability": 0.84560546875}, {"start": 2168.66, "end": 2168.9, "word": " في", "probability": 0.95556640625}, {"start": 2168.9, "end": 2169.72, "word": " ظروف", "probability": 0.9737548828125}, {"start": 2169.72, "end": 2170.68, "word": " unaerobic", "probability": 0.695556640625}, {"start": 2170.68, "end": 2171.34, "word": " condition", "probability": 0.97509765625}, {"start": 2171.34, "end": 2172.46, "word": " بشكل", "probability": 0.9869791666666666}, {"start": 2172.46, "end": 2173.56, "word": " اضطراري", "probability": 0.934814453125}, {"start": 2173.56, "end": 2174.18, "word": " يبقى", "probability": 0.96533203125}, {"start": 2174.18, "end": 2174.6, "word": " هان", "probability": 0.814453125}, {"start": 2174.6, "end": 2175.22, "word": " صار", "probability": 0.924560546875}, {"start": 2175.22, "end": 2175.4, "word": " عند", "probability": 0.93603515625}, {"start": 2175.4, "end": 2176.24, "word": " deamination", "probability": 0.7657877604166666}, {"start": 2176.24, "end": 2177.36, "word": " للبيبتون", "probability": 0.92431640625}, {"start": 2177.36, "end": 2177.62, "word": " في", "probability": 0.982421875}, {"start": 2177.62, "end": 2178.2, "word": " ظروف", "probability": 0.96826171875}, {"start": 2178.2, "end": 2178.88, "word": " unaerobic", "probability": 0.9069010416666666}, {"start": 2178.88, "end": 2179.42, "word": " condition", "probability": 0.98095703125}, {"start": 2179.42, "end": 2179.9, "word": " لكن", "probability": 0.884765625}], "temperature": 1.0}, {"id": 89, "seek": 219986, "start": 2180.32, "end": 2199.86, "text": "بشكل اضطراري يبقى لو كانت البكتيريا non-gelicose fermenter ممكن تكون red على not change نكتب K على not change أو red على red اللي هي K على K يبقى هان النتيجة نكتبها K", "tokens": [3555, 8592, 28820, 1975, 11242, 9566, 2288, 9640, 1829, 7251, 3555, 4587, 7578, 45164, 25961, 2655, 29739, 4117, 2655, 13546, 25528, 2107, 12, 10345, 299, 541, 38300, 260, 3714, 43020, 6055, 30544, 2182, 15844, 406, 1319, 8717, 4117, 2655, 3555, 591, 15844, 406, 1319, 34051, 2182, 15844, 2182, 13672, 1829, 39896, 591, 15844, 591, 7251, 3555, 4587, 7578, 8032, 7649, 28239, 31371, 7435, 3660, 8717, 4117, 2655, 3555, 11296, 591], "avg_logprob": -0.1837588070144116, "compression_ratio": 1.5121951219512195, "no_speech_prob": 3.808736801147461e-05, "words": [{"start": 2180.32, "end": 2180.94, "word": "بشكل", "probability": 0.9765625}, {"start": 2180.94, "end": 2181.88, "word": " اضطراري", "probability": 0.8868001302083334}, {"start": 2181.88, "end": 2182.56, "word": " يبقى", "probability": 0.9586181640625}, {"start": 2182.56, "end": 2182.74, "word": " لو", "probability": 0.96337890625}, {"start": 2182.74, "end": 2183.08, "word": " كانت", "probability": 0.98681640625}, {"start": 2183.08, "end": 2183.6, "word": " البكتيريا", "probability": 0.90693359375}, {"start": 2183.6, "end": 2183.82, "word": " non", "probability": 0.306884765625}, {"start": 2183.82, "end": 2184.26, "word": "-gelicose", "probability": 0.779052734375}, {"start": 2184.26, "end": 2184.74, "word": " fermenter", "probability": 0.570556640625}, {"start": 2184.74, "end": 2184.98, "word": " ممكن", "probability": 0.89501953125}, {"start": 2184.98, "end": 2185.44, "word": " تكون", "probability": 0.950439453125}, {"start": 2185.44, "end": 2185.92, "word": " red", "probability": 0.395263671875}, {"start": 2185.92, "end": 2186.3, "word": " على", "probability": 0.89013671875}, {"start": 2186.3, "end": 2187.44, "word": " not", "probability": 0.697265625}, {"start": 2187.44, "end": 2188.04, "word": " change", "probability": 0.66357421875}, {"start": 2188.04, "end": 2189.56, "word": " نكتب", "probability": 0.885009765625}, {"start": 2189.56, "end": 2190.66, "word": " K", "probability": 0.33642578125}, {"start": 2190.66, "end": 2191.0, "word": " على", "probability": 0.86474609375}, {"start": 2191.0, "end": 2191.32, "word": " not", "probability": 0.900390625}, {"start": 2191.32, "end": 2191.76, "word": " change", "probability": 0.80712890625}, {"start": 2191.76, "end": 2192.1, "word": " أو", "probability": 0.68798828125}, {"start": 2192.1, "end": 2192.4, "word": " red", "probability": 0.76611328125}, {"start": 2192.4, "end": 2192.64, "word": " على", "probability": 0.94091796875}, {"start": 2192.64, "end": 2192.88, "word": " red", "probability": 0.8154296875}, {"start": 2192.88, "end": 2193.04, "word": " اللي", "probability": 0.800537109375}, {"start": 2193.04, "end": 2193.44, "word": " هي", "probability": 0.8896484375}, {"start": 2193.44, "end": 2194.6, "word": " K", "probability": 0.85986328125}, {"start": 2194.6, "end": 2194.8, "word": " على", "probability": 0.92578125}, {"start": 2194.8, "end": 2195.06, "word": " K", "probability": 0.9638671875}, {"start": 2195.06, "end": 2195.24, "word": " يبقى", "probability": 0.9300537109375}, {"start": 2195.24, "end": 2195.48, "word": " هان", "probability": 0.6845703125}, {"start": 2195.48, "end": 2195.92, "word": " 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"probability": 0.98486328125}, {"start": 2311.59, "end": 2312.35, "word": " نتيجة", "probability": 0.990966796875}, {"start": 2312.35, "end": 2312.91, "word": " أشبهون", "probability": 0.81474609375}, {"start": 2312.91, "end": 2313.23, "word": " لونها", "probability": 0.9742838541666666}, {"start": 2313.23, "end": 2313.41, "word": " أو", "probability": 0.86474609375}, {"start": 2313.41, "end": 2313.63, "word": " كيف", "probability": 0.9638671875}, {"start": 2313.63, "end": 2313.95, "word": " بيكون", "probability": 0.8839518229166666}, {"start": 2313.95, "end": 2314.43, "word": " الشكل", "probability": 0.991455078125}, {"start": 2314.43, "end": 2314.73, "word": " لكن", "probability": 0.85888671875}, {"start": 2314.73, "end": 2315.41, "word": " بالنسبة", "probability": 0.9962158203125}, {"start": 2315.41, "end": 2315.73, "word": " لـ", "probability": 0.837158203125}], "temperature": 1.0}, {"id": 94, "seek": 234303, "start": 2316.01, "end": 2343.03, "text": "الـ H2S production طيب لو تم إنتاج اللون الأسود احنا قلنا الـ H2S production بتكون عندي black precipitate هل هقدر أشوف طبعا الـ H2S production بيصير زي ما قلنا قبل الـ H2S production قلنا فيه بيصير في ظروف أن European condition بيكون في البط", "tokens": [6027, 39184, 389, 17, 50, 4265, 23032, 1829, 3555, 45164, 46811, 11933, 29399, 26108, 13672, 11536, 16247, 3794, 23328, 1975, 5016, 8315, 12174, 1211, 8315, 2423, 39184, 389, 17, 50, 4265, 39894, 30544, 18871, 16254, 2211, 23354, 8086, 8032, 1211, 8032, 28543, 2288, 5551, 8592, 38688, 23032, 3555, 3615, 995, 2423, 39184, 389, 17, 50, 4265, 4724, 1829, 9381, 13546, 30767, 1829, 19446, 12174, 1211, 8315, 12174, 36150, 2423, 39184, 389, 17, 50, 4265, 12174, 1211, 8315, 8978, 3224, 4724, 1829, 9381, 13546, 8978, 1357, 116, 32887, 5172, 14739, 6473, 4188, 4724, 1829, 30544, 8978, 29739, 9566], "avg_logprob": -0.16470025662256746, "compression_ratio": 1.7438423645320198, "no_speech_prob": 1.0609626770019531e-05, "words": [{"start": 2316.01, "end": 2316.41, "word": 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2322.59, "word": " H2S", "probability": 0.9083658854166666}, {"start": 2322.59, "end": 2323.39, "word": " production", "probability": 0.974609375}, {"start": 2323.39, "end": 2324.21, "word": " بتكون", "probability": 0.924072265625}, {"start": 2324.21, "end": 2324.69, "word": " عندي", "probability": 0.6527099609375}, {"start": 2324.69, "end": 2325.27, "word": " black", "probability": 0.52490234375}, {"start": 2325.27, "end": 2326.09, "word": " precipitate", "probability": 0.94091796875}, {"start": 2326.09, "end": 2327.07, "word": " هل", "probability": 0.973876953125}, {"start": 2327.07, "end": 2327.61, "word": " هقدر", "probability": 0.98974609375}, {"start": 2327.61, "end": 2328.01, "word": " أشوف", "probability": 0.8494466145833334}, {"start": 2328.01, "end": 2328.39, "word": " طبعا", "probability": 0.9775390625}, {"start": 2328.39, "end": 2328.83, "word": " الـ", "probability": 0.91943359375}, {"start": 2328.83, "end": 2329.53, "word": " H2S", "probability": 0.9375}, {"start": 2329.53, "end": 2330.23, "word": " production", "probability": 0.978515625}, {"start": 2330.23, "end": 2330.83, "word": " بيصير", "probability": 0.9326171875}, {"start": 2330.83, "end": 2331.07, "word": " زي", "probability": 0.9716796875}, {"start": 2331.07, "end": 2331.17, "word": " ما", "probability": 0.99169921875}, {"start": 2331.17, "end": 2331.49, "word": " قلنا", "probability": 0.89111328125}, {"start": 2331.49, "end": 2331.87, "word": " قبل", "probability": 0.989990234375}, {"start": 2331.87, "end": 2337.85, "word": " الـ", "probability": 0.965087890625}, {"start": 2337.85, "end": 2338.41, "word": " H2S", "probability": 0.92919921875}, {"start": 2338.41, "end": 2338.93, "word": " production", "probability": 0.9755859375}, {"start": 2338.93, "end": 2339.27, "word": " قلنا", "probability": 0.9366861979166666}, {"start": 2339.27, "end": 2339.57, "word": " فيه", "probability": 0.709228515625}, {"start": 2339.57, "end": 2339.91, "word": " بيصير", "probability": 0.972900390625}, {"start": 2339.91, "end": 2340.05, "word": " في", "probability": 0.96728515625}, {"start": 2340.05, "end": 2340.53, "word": " ظروف", "probability": 0.9622802734375}, {"start": 2340.53, "end": 2340.87, "word": " أن", "probability": 0.11419677734375}, {"start": 2340.87, "end": 2341.23, "word": " European", "probability": 0.189453125}, {"start": 2341.23, "end": 2341.83, "word": " condition", "probability": 0.83935546875}, {"start": 2341.83, "end": 2342.31, "word": " بيكون", "probability": 0.7937825520833334}, {"start": 2342.31, "end": 2342.57, "word": " في", "probability": 0.970703125}, {"start": 2342.57, "end": 2343.03, "word": " البط", "probability": 0.964111328125}], "temperature": 1.0}, {"id": 95, "seek": 237060, "start": 2344.44, "end": 2370.6, "text": "لو صار في عندي إنتاج اللون الأسود هل هقدر أشوف اللون الأصفر الناتج من عملية ال fermentation خاصة أن ال fermentation و ال H2S production بيصيروا في الـ Un-aerobic condition بيصيروا في البط لو صار في عندي تكون للون الأسود الناتج من أنه اتكون ال H2S gas هل هقدر أشوف اللون الأصفر في البط ولا لأ؟ بقوللي", "tokens": [1211, 2407, 20328, 9640, 8978, 18871, 16254, 11933, 29399, 26108, 13672, 11536, 16247, 3794, 23328, 8032, 1211, 8032, 28543, 2288, 5551, 8592, 38688, 13672, 11536, 16247, 9381, 5172, 2288, 28239, 9307, 7435, 9154, 6225, 42213, 10632, 2423, 43161, 16490, 33546, 3660, 14739, 2423, 43161, 4032, 2423, 389, 17, 50, 4265, 4724, 1829, 9381, 13546, 14407, 8978, 2423, 39184, 1156, 12, 64, 260, 22234, 4188, 4724, 1829, 9381, 13546, 14407, 8978, 29739, 9566, 45164, 20328, 9640, 8978, 18871, 16254, 6055, 30544, 24976, 11536, 16247, 3794, 23328, 28239, 9307, 7435, 9154, 14739, 3224, 1975, 2655, 30544, 2423, 389, 17, 50, 4211, 8032, 1211, 8032, 28543, 2288, 5551, 8592, 38688, 13672, 11536, 16247, 9381, 5172, 2288, 8978, 29739, 9566, 49429, 5296, 10721, 22807, 4724, 39648, 20292], "avg_logprob": -0.16645665707126742, "compression_ratio": 2.142857142857143, "no_speech_prob": 5.543231964111328e-06, "words": [{"start": 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0.82861328125}, {"start": 2365.04, "end": 2365.8, "word": " هل", "probability": 0.987060546875}, {"start": 2365.8, "end": 2366.22, "word": " هقدر", "probability": 0.9964192708333334}, {"start": 2366.22, "end": 2366.7, "word": " أشوف", "probability": 0.9700520833333334}, {"start": 2366.7, "end": 2367.28, "word": " اللون", "probability": 0.998046875}, {"start": 2367.28, "end": 2368.1, "word": " الأصفر", "probability": 0.937255859375}, {"start": 2368.1, "end": 2368.3, "word": " في", "probability": 0.99169921875}, {"start": 2368.3, "end": 2368.74, "word": " البط", "probability": 0.987060546875}, {"start": 2368.74, "end": 2368.92, "word": " ولا", "probability": 0.91455078125}, {"start": 2368.92, "end": 2369.74, "word": " لأ؟", "probability": 0.7943522135416666}, {"start": 2369.74, "end": 2370.6, "word": " بقوللي", "probability": 0.7972005208333334}], "temperature": 1.0}, {"id": 96, "seek": 239302, "start": 2370.9, "end": 2393.02, "text": "لو عملت البكتيريا H2S production بدي أكون عارفة إنه عملت Gelicose fermentation يبقى طالما البكتيريا H2S production positive يبقى هي Gelicose fermentation positive لأن إنتاج الـH2S production يحصل في الوسط الحمضي", "tokens": [1211, 2407, 6225, 42213, 2655, 29739, 4117, 2655, 13546, 25528, 389, 17, 50, 4265, 4724, 16254, 5551, 30544, 6225, 9640, 5172, 3660, 36145, 3224, 6225, 42213, 2655, 16142, 299, 541, 43161, 7251, 3555, 4587, 7578, 23032, 6027, 15042, 29739, 4117, 2655, 13546, 25528, 389, 17, 50, 4265, 3353, 7251, 3555, 4587, 7578, 39896, 16142, 299, 541, 43161, 3353, 5296, 33456, 11933, 29399, 26108, 2423, 39184, 39, 17, 50, 4265, 7251, 5016, 36520, 8978, 2423, 41779, 9566, 21542, 2304, 11242, 1829], "avg_logprob": -0.16560570693310397, "compression_ratio": 1.6685393258426966, "no_speech_prob": 2.384185791015625e-07, "words": [{"start": 2370.9, "end": 2371.26, "word": "لو", "probability": 0.903564453125}, {"start": 2371.26, "end": 2371.72, "word": " عملت", "probability": 0.9404296875}, {"start": 2371.72, "end": 2372.44, "word": " 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كانت lactose fermenter أو non-lactose fermenter بتكون A على A لأنه فقط بيتم استهلاك الجليكوز خلال أول 12 ساعة بيتحول بيتكون acid هذا النواتج عملية ال fermentation acid بيصير في الان European condition لكن اللون الأصفر بيمتد في كل tube فبالتالي هفكر النتيجة A على A", "tokens": [4587, 36150, 4022, 8608, 995, 27884, 12174, 36150, 4022, 8608, 995, 27884, 12174, 1211, 8315, 8608, 2407, 16606, 25961, 2655, 34042, 541, 38300, 260, 34051, 2107, 12, 75, 578, 541, 38300, 260, 39894, 30544, 316, 15844, 316, 5296, 33456, 3224, 6156, 47432, 4724, 36081, 2304, 44713, 3224, 15040, 4117, 25724, 20292, 4117, 2407, 11622, 16490, 1211, 6027, 5551, 12610, 2272, 8608, 995, 27884, 4724, 36081, 5016, 12610, 4724, 36081, 30544, 8258, 23758, 28239, 2407, 9307, 7435, 6225, 42213, 10632, 2423, 43161, 8258, 4724, 1829, 9381, 13546, 8978, 2423, 7649, 6473, 4188, 44381, 13672, 11536, 16247, 9381, 5172, 2288, 4724, 32640, 2655, 3215, 8978, 28242, 9917, 6156, 3555, 6027, 2655, 6027, 1829, 8032, 5172, 37983, 28239, 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2747.14, "text": "Iron بدي أكشف على الـ H2S production و الأجار لأن فيها نسبة أجار واحد و نصف في الميديا واحد و نصف بالمية Cligar iron agar and triple sugar iron agar are widely used in the identification of gram negative bacteria particularly the enterobacteriasis هذه الميديا بتستخدم للتعريف للgram negative bacteria خاصة ال enterobacteriasis", "tokens": [40, 2044, 4724, 16254, 5551, 4117, 8592, 5172, 15844, 2423, 39184, 389, 17, 50, 4265, 4032, 16247, 7435, 9640, 5296, 33456, 8978, 11296, 8717, 35457, 3660, 5551, 7435, 9640, 36764, 24401, 4032, 8717, 9381, 5172, 8978, 9673, 1829, 16254, 995, 36764, 24401, 4032, 8717, 9381, 5172, 20666, 2304, 10632, 2033, 328, 289, 6497, 623, 289, 293, 15508, 5076, 6497, 623, 289, 366, 13371, 1143, 294, 264, 22065, 295, 21353, 3671, 11763, 4098, 264, 3242, 996, 14125, 4609, 271, 29538, 9673, 1829, 16254, 995, 39894, 14851, 9778, 40448, 24976, 2655, 3615, 16572, 5172, 24976, 1342, 3671, 11763, 16490, 33546, 3660, 2423, 3242, 996, 14125, 4609, 271], 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" the", "probability": 0.5009765625}, {"start": 2733.84, "end": 2734.54, "word": " identification", "probability": 0.9404296875}, {"start": 2734.54, "end": 2735.18, "word": " of", "probability": 0.9765625}, {"start": 2735.18, "end": 2735.72, "word": " gram", "probability": 0.6435546875}, {"start": 2735.72, "end": 2736.14, "word": " negative", "probability": 0.65380859375}, {"start": 2736.14, "end": 2736.8, "word": " bacteria", "probability": 0.935546875}, {"start": 2736.8, "end": 2737.76, "word": " particularly", "probability": 0.74169921875}, {"start": 2737.76, "end": 2738.92, "word": " the", "probability": 0.87744140625}, {"start": 2738.92, "end": 2740.12, "word": " enterobacteriasis", "probability": 0.659228515625}, {"start": 2740.12, "end": 2740.5, "word": " هذه", "probability": 0.6259765625}, {"start": 2740.5, "end": 2741.22, "word": " الميديا", "probability": 0.958251953125}, {"start": 2741.22, "end": 2742.58, "word": " بتستخدم", "probability": 0.93212890625}, {"start": 2742.58, 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بكشف على ال fermentation احنا قولنا ان في هاي ال media انا بكشف على ال fermentation هلأ البكتيريا هتستهلك اول ما هتروح هتروح ل", "tokens": [1424, 21961, 781, 337, 38300, 1166, 24128, 17186, 3082, 307, 264, 700, 5076, 3857, 14923, 1602, 4724, 39648, 20292, 8978, 8032, 47302, 2423, 3021, 1975, 8315, 4724, 4117, 8592, 5172, 15844, 2423, 43161, 1975, 5016, 8315, 12174, 12610, 8315, 16472, 8978, 8032, 47302, 2423, 3021, 1975, 8315, 4724, 4117, 8592, 5172, 15844, 2423, 43161, 8032, 1211, 10721, 29739, 4117, 2655, 13546, 25528, 8032, 2655, 14851, 3224, 23275, 1975, 12610, 19446, 8032, 2655, 32887, 5016, 8032, 2655, 32887, 5016, 5296], "avg_logprob": -0.2378906285390258, "compression_ratio": 1.7595628415300546, "no_speech_prob": 8.940696716308594e-07, "words": [{"start": 3093.19, "end": 3093.97, "word": "principle", "probability": 0.6019083658854166}, {"start": 3093.97, "end": 3094.31, "word": " for", "probability": 0.853515625}, {"start": 3094.31, "end": 3095.11, "word": " fermentative", 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yellow", "probability": 0.708984375}], "temperature": 1.0}, {"id": 132, "seek": 331248, "start": 3287.72, "end": 3312.48, "text": "فبالتالي هذا هيصير في الـ Un-aerobic condition و هيمتد اللون الأصفر في كل سيوب فبالتالي كل الـ media هيكون لونها أصفر هيج أنا leading one to conclude that the organism is a lactose fermenter when it simply may not yet have exercised the glycose بقوللي أنا هفكر أنه ال organism صار عنده", "tokens": [5172, 3555, 6027, 2655, 6027, 1829, 23758, 39896, 9381, 13546, 8978, 2423, 39184, 1156, 12, 64, 260, 22234, 4188, 4032, 39896, 2304, 2655, 3215, 13672, 11536, 16247, 9381, 5172, 2288, 8978, 28242, 8608, 1829, 37746, 6156, 3555, 6027, 2655, 6027, 1829, 28242, 2423, 39184, 3021, 39896, 30544, 5296, 11536, 11296, 5551, 9381, 5172, 2288, 39896, 7435, 41850, 5775, 472, 281, 16886, 300, 264, 24128, 307, 257, 34042, 541, 38300, 260, 562, 309, 2935, 815, 406, 1939, 362, 454, 2869, 2640, 264, 290, 356, 6877, 68, 4724, 39648, 20292, 41850, 8032, 5172, 37983, 14739, 3224, 2423, 24128, 20328, 9640, 43242, 3224], "avg_logprob": -0.27645422090398203, "compression_ratio": 1.5436507936507937, "no_speech_prob": 2.384185791015625e-07, "words": [{"start": 3287.72, "end": 3288.64, "word": "فبالتالي", "probability": 0.9080403645833334}, {"start": 3288.64, "end": 3289.1, "word": " هذا", "probability": 0.58349609375}, {"start": 3289.1, "end": 3289.56, "word": " هيصير", "probability": 0.7605794270833334}, {"start": 3289.56, "end": 3289.74, "word": " في", "probability": 0.8203125}, {"start": 3289.74, "end": 3290.1, "word": " الـ", "probability": 0.53070068359375}, {"start": 3290.1, "end": 3290.26, "word": " Un", "probability": 0.2166748046875}, {"start": 3290.26, "end": 3290.6, "word": "-aerobic", "probability": 0.5557861328125}, {"start": 3290.6, "end": 3291.02, "word": " condition", "probability": 0.96484375}, {"start": 3291.02, "end": 3291.18, "word": " و", "probability": 0.481201171875}, {"start": 3291.18, "end": 3291.64, "word": " هيمتد", "probability": 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{"start": 3295.8, "end": 3296.64, "word": " هيج", "probability": 0.43804931640625}, {"start": 3296.64, "end": 3296.98, "word": " أنا", "probability": 0.67822265625}, {"start": 3296.98, "end": 3297.52, "word": " leading", "probability": 0.931640625}, {"start": 3297.52, "end": 3297.94, "word": " one", "probability": 0.849609375}, {"start": 3297.94, "end": 3298.26, "word": " to", "probability": 0.94970703125}, {"start": 3298.26, "end": 3298.86, "word": " conclude", "probability": 0.54150390625}, {"start": 3298.86, "end": 3299.54, "word": " that", "probability": 0.90380859375}, {"start": 3299.54, "end": 3299.92, "word": " the", "probability": 0.7802734375}, {"start": 3299.92, "end": 3300.4, "word": " organism", "probability": 0.9892578125}, {"start": 3300.4, "end": 3300.74, "word": " is", "probability": 0.97265625}, {"start": 3300.74, "end": 3300.9, "word": " a", "probability": 0.98486328125}, {"start": 3300.9, "end": 3301.46, "word": " lactose", "probability": 0.942626953125}, {"start": 3301.46, "end": 3302.2, "word": " fermenter", "probability": 0.750244140625}, {"start": 3302.2, "end": 3302.64, "word": " when", "probability": 0.88623046875}, {"start": 3302.64, "end": 3302.92, "word": " it", "probability": 0.96923828125}, {"start": 3302.92, "end": 3303.36, "word": " simply", "probability": 0.82861328125}, {"start": 3303.36, "end": 3303.7, "word": " may", "probability": 0.931640625}, {"start": 3303.7, "end": 3304.14, "word": " not", "probability": 0.966796875}, {"start": 3304.14, "end": 3304.74, "word": " yet", "probability": 0.91015625}, {"start": 3304.74, "end": 3305.18, "word": " have", "probability": 0.9248046875}, {"start": 3305.18, "end": 3306.28, "word": " exercised", "probability": 0.5838216145833334}, {"start": 3306.28, "end": 3306.68, "word": " the", "probability": 0.91015625}, {"start": 3306.68, "end": 3307.34, "word": " glycose", "probability": 0.55657958984375}, {"start": 3307.34, "end": 3307.86, "word": " بقوللي", "probability": 0.6787923177083334}, {"start": 3307.86, "end": 3308.42, "word": " أنا", "probability": 0.70556640625}, {"start": 3308.42, "end": 3309.1, "word": " هفكر", "probability": 0.9861653645833334}, {"start": 3309.1, "end": 3309.66, "word": " أنه", "probability": 0.681396484375}, {"start": 3309.66, "end": 3309.82, "word": " ال", "probability": 0.72021484375}, {"start": 3309.82, "end": 3310.4, "word": " organism", "probability": 0.71142578125}, {"start": 3310.4, "end": 3311.96, "word": " صار", "probability": 0.986083984375}, {"start": 3311.96, "end": 3312.48, "word": " عنده", "probability": 0.99609375}], "temperature": 1.0}, {"id": 133, "seek": 333161, "start": 3312.75, "end": 3331.61, "text": "أو عمل fermentation للـ lactose لكن هو فقط لحد الآن استهلك الجليكوز لو أنا قرأت بعد 24 ساعة a reading after the لو أنا قرأت بعد 24 ساعة the lactose has been depleted بقولني أن ال lactose بسله استهلاك كله", "tokens": [10721, 2407, 6225, 42213, 43161, 24976, 39184, 34042, 541, 44381, 31439, 6156, 47432, 5296, 24401, 6024, 48506, 44713, 3224, 23275, 25724, 20292, 4117, 2407, 11622, 45164, 41850, 12174, 2288, 10721, 2655, 39182, 4022, 8608, 995, 27884, 257, 3760, 934, 264, 45164, 41850, 12174, 2288, 10721, 2655, 39182, 4022, 8608, 995, 27884, 264, 34042, 541, 575, 668, 37546, 10993, 4724, 39648, 22653, 14739, 2423, 34042, 541, 4724, 3794, 43761, 44713, 3224, 15040, 4117, 28242, 3224], "avg_logprob": -0.20447916746139527, "compression_ratio": 1.6, "no_speech_prob": 1.7881393432617188e-06, "words": [{"start": 3312.75, "end": 3313.05, "word": "أو", "probability": 0.865234375}, {"start": 3313.05, "end": 3313.31, "word": " عمل", "probability": 0.9755859375}, {"start": 3313.31, "end": 3313.95, "word": " fermentation", "probability": 0.931640625}, {"start": 3313.95, "end": 3314.65, "word": " للـ", "probability": 0.6143798828125}, {"start": 3314.65, "end": 3315.11, "word": " lactose", "probability": 0.75927734375}, {"start": 3315.11, "end": 3315.43, "word": " لكن", "probability": 0.8291015625}, {"start": 3315.43, "end": 3315.77, "word": " هو", "probability": 0.9482421875}, {"start": 3315.77, "end": 3316.21, "word": " فقط", "probability": 0.95703125}, {"start": 3316.21, "end": 3316.57, "word": " لحد", "probability": 0.902587890625}, {"start": 3316.57, "end": 3317.15, "word": " الآن", "probability": 0.6685791015625}, {"start": 3317.15, "end": 3318.19, "word": " استهلك", "probability": 0.89111328125}, {"start": 3318.19, "end": 3318.93, "word": " الجليكوز", "probability": 0.808935546875}, {"start": 3318.93, "end": 3319.75, "word": " لو", "probability": 0.86376953125}, {"start": 3319.75, "end": 3320.03, "word": " أنا", "probability": 0.79248046875}, {"start": 3320.03, "end": 3320.55, "word": " قرأت", "probability": 0.987548828125}, {"start": 3320.55, "end": 3320.77, "word": " بعد", "probability": 0.970703125}, {"start": 3320.77, "end": 3321.33, "word": " 24", "probability": 0.86572265625}, {"start": 3321.33, "end": 3322.15, "word": " ساعة", "probability": 0.8994140625}, {"start": 3322.15, "end": 3322.59, "word": " a", "probability": 0.5498046875}, {"start": 3322.59, "end": 3322.95, "word": " reading", "probability": 0.95703125}, {"start": 3322.95, "end": 3323.51, "word": " after", "probability": 0.88818359375}, {"start": 3323.51, "end": 3323.87, "word": " the", "probability": 0.4208984375}, {"start": 3323.87, "end": 3324.45, "word": " لو", "probability": 0.392333984375}, {"start": 3324.45, "end": 3324.67, "word": " أنا", "probability": 0.82177734375}, {"start": 3324.67, "end": 3325.01, "word": " قرأت", "probability": 0.9959716796875}, {"start": 3325.01, "end": 3325.17, "word": " بعد", "probability": 0.970703125}, {"start": 3325.17, "end": 3325.61, "word": " 24", "probability": 0.9873046875}, {"start": 3325.61, "end": 3326.09, "word": " ساعة", "probability": 0.89990234375}, {"start": 3326.09, "end": 3326.21, "word": " the", "probability": 0.35546875}, {"start": 3326.21, "end": 3326.79, "word": " lactose", "probability": 0.97412109375}, {"start": 3326.79, "end": 3327.17, "word": " has", "probability": 0.87255859375}, {"start": 3327.17, "end": 3327.49, "word": " been", "probability": 0.9267578125}, {"start": 3327.49, "end": 3328.17, "word": " depleted", "probability": 0.861083984375}, {"start": 3328.17, "end": 3328.61, "word": " بقولني", "probability": 0.7357584635416666}, {"start": 3328.61, "end": 3328.77, "word": " أن", "probability": 0.385498046875}, {"start": 3328.77, "end": 3328.91, "word": " ال", "probability": 0.435791015625}, {"start": 3328.91, "end": 3329.49, "word": " lactose", "probability": 0.80908203125}, {"start": 3329.49, "end": 3330.07, "word": " بسله", "probability": 0.94873046875}, {"start": 3330.07, "end": 3331.13, "word": " استهلاك", "probability": 0.8870849609375}, {"start": 3331.13, "end": 3331.61, "word": " كله", "probability": 0.982666015625}], "temperature": 1.0}, {"id": 134, "seek": 336166, "start": 3332.52, "end": 3361.66, "text": "could reveal a yellow pot and red slant leading one to fossil conclude that the organism is glucose only fermenter لو أنا قرأت بعد 24 ساعات لكتاز والسكراز هيتم استهلاكهم فبالتالي البكتيريا هتروح لمصادر تانية اللي هي ال بيبتون فبالتالي هيصير في عندي deamination للبيبتون في ال slant هيتحول لون ال slant للون red لكن في ال pot هيكون yellow فبالتالي انا هفكر هذه النتيجة K على A ان فقط البكتيريا", "tokens": [66, 429, 10658, 257, 5566, 1847, 293, 2182, 1061, 394, 5775, 472, 281, 18737, 16886, 300, 264, 24128, 307, 1563, 1311, 541, 787, 38300, 260, 45164, 41850, 12174, 2288, 10721, 2655, 39182, 4022, 8608, 45761, 9307, 5296, 4117, 2655, 31377, 16070, 3794, 37983, 31377, 8032, 36081, 2304, 44713, 3224, 15040, 4117, 16095, 6156, 3555, 6027, 2655, 6027, 1829, 29739, 4117, 2655, 13546, 25528, 8032, 2655, 32887, 5016, 32767, 9381, 18513, 2288, 6055, 7649, 10632, 13672, 1829, 39896, 2423, 4724, 1829, 3555, 2655, 11536, 6156, 3555, 6027, 2655, 6027, 1829, 8032, 1829, 9381, 13546, 8978, 18871, 16254, 368, 335, 2486, 24976, 21292, 3555, 2655, 11536, 8978, 2423, 1061, 394, 8032, 36081, 5016, 12610, 5296, 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3806.1, "end": 3806.32, "word": " أنه", "probability": 0.91015625}, {"start": 3806.32, "end": 3806.58, "word": " لونه", "probability": 0.7560221354166666}, {"start": 3806.58, "end": 3806.9, "word": " أحمر", "probability": 0.98095703125}, {"start": 3806.9, "end": 3807.32, "word": " فوق", "probability": 0.67138671875}, {"start": 3807.32, "end": 3807.72, "word": " على", "probability": 0.9267578125}, {"start": 3807.72, "end": 3808.82, "word": " A", "probability": 0.9755859375}], "temperature": 1.0}, {"id": 153, "seek": 383149, "start": 3809.07, "end": 3831.49, "text": "طبعا لأنه في اندي H2S production فعلى طول بعرف أنه اللي تحت اللون المفترض يكون أصفر اللي هو A fermentation مع H2S هذا بالنسبة لأول واحدة", "tokens": [9566, 3555, 3615, 995, 5296, 33456, 3224, 8978, 16472, 16254, 389, 17, 50, 4265, 6156, 3615, 23942, 23032, 12610, 45030, 28480, 14739, 3224, 13672, 1829, 6055, 33753, 13672, 11536, 9673, 5172, 2655, 43042, 7251, 30544, 5551, 9381, 5172, 2288, 13672, 1829, 31439, 316, 43161, 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Iron", "probability": 0.92138671875}, {"start": 4013.03, "end": 4013.43, "word": " Agar", "probability": 0.89990234375}, {"start": 4013.43, "end": 4013.69, "word": " هكذا", "probability": 0.7118326822916666}, {"start": 4013.69, "end": 4013.93, "word": " نكون", "probability": 0.4248046875}, {"start": 4013.93, "end": 4014.51, "word": " خلصنا", "probability": 0.94482421875}, {"start": 4014.51, "end": 4014.85, "word": " أول", "probability": 0.89501953125}, {"start": 4014.85, "end": 4015.41, "word": " ميديتين", "probability": 0.7408203125}, {"start": 4015.41, "end": 4015.63, "word": " الـ", "probability": 0.61181640625}, {"start": 4015.63, "end": 4016.23, "word": " SIM", "probability": 0.6136474609375}, {"start": 4016.23, "end": 4016.71, "word": " و", "probability": 0.8388671875}, {"start": 4016.71, "end": 4017.13, "word": " Triple", "probability": 0.44140625}, {"start": 4017.13, "end": 4017.65, "word": " Sugar", "probability": 0.8994140625}, {"start": 4017.65, "end": 4018.07, "word": " Iron", "probability": 0.88525390625}, {"start": 4018.07, "end": 4018.53, "word": " Agar", "probability": 0.945556640625}, {"start": 4018.53, "end": 4018.89, "word": " أو", "probability": 0.77734375}, {"start": 4018.89, "end": 4019.59, "word": " Trigger", "probability": 0.36578369140625}, {"start": 4019.59, "end": 4019.97, "word": " Sugar", "probability": 0.7392578125}, {"start": 4019.97, "end": 4022.21, "word": " Iron", "probability": 0.8671875}, {"start": 4022.21, "end": 4022.81, "word": " Agar", "probability": 0.95849609375}], "temperature": 1.0}], "language": "ar", "language_probability": 1.0, "duration": 4024.598625, "duration_after_vad": 3888.065624999981}
\ No newline at end of file
diff --git a/PL9fwy3NUQKwZWgAb_V1ATlRRsH-6i3cPC/owBu12fuBog_raw.srt b/PL9fwy3NUQKwZWgAb_V1ATlRRsH-6i3cPC/owBu12fuBog_raw.srt
new file mode 100644
index 0000000000000000000000000000000000000000..038f6ebd4421903e3a32d2aa4dfab2a1cb537988
--- /dev/null
+++ b/PL9fwy3NUQKwZWgAb_V1ATlRRsH-6i3cPC/owBu12fuBog_raw.srt
@@ -0,0 +1,3568 @@
+1
+00:00:03,170 --> 00:00:07,030
+السلام عليكم ورحمة الله وبركاته يعطيكم العافية
+
+2
+00:00:07,030 --> 00:00:11,070
+جميعا نستكمل معكم سلسلة محاضرات مساق الـ
+
+3
+00:00:11,070 --> 00:00:15,390
+Microbiology العملي في هذا اللقاء هتكون المحاضرة
+
+4
+00:00:15,390 --> 00:00:19,810
+بعنوان Single Media Multiple Test في المعامل
+
+5
+00:00:19,810 --> 00:00:24,990
+السابقة كنا نستخدم ميديا بتكشف على فحص كيميائي
+
+6
+00:00:24,990 --> 00:00:29,680
+واحد one biochemical testاليوم هنتعامل مع ميديا
+
+7
+00:00:29,680 --> 00:00:34,240
+هتكشف على أكتر من biochemical test بقدر أعمل فيها
+
+8
+00:00:34,240 --> 00:00:39,060
+أكتر من فحص كيميائي من الأمثلة على high end media
+
+9
+00:00:39,060 --> 00:00:43,980
+في عندي أكتر من ميديا بقدر من خلالها أكشف على أكتر
+
+10
+00:00:43,980 --> 00:00:49,440
+من biochemical test SIM media trigger iron agar
+
+11
+00:00:49,860 --> 00:00:54,300
+Triple Sugar Iron Agar Lysine Iron Agar Motility
+
+12
+00:00:54,300 --> 00:00:59,360
+Indole Ornithine Media يبقى هاي الميديا من الأمثلة
+
+13
+00:00:59,360 --> 00:01:04,720
+على Single Media Multiple Test اسم الميديا جاي من
+
+14
+00:01:04,720 --> 00:01:08,360
+الفحوصات اللي أنا بقدر أعملها في هاي الميديا
+
+15
+00:01:08,360 --> 00:01:13,160
+هنتعرف في هذا المعمل على كل ميديا من هاي الميديات
+
+16
+00:01:13,160 --> 00:01:17,630
+بالتفصيلشو الفحوصات اللى بتكشف عنها كل ميديا كيف
+
+17
+00:01:17,630 --> 00:01:23,930
+أنا بقدر أفسر نتائج هذه الميديا خلينا نبدأ بأول
+
+18
+00:01:23,930 --> 00:01:28,690
+ميديا اللى هى الـ SIM media قولنا إن اسم الميديا
+
+19
+00:01:28,690 --> 00:01:32,850
+جاى من أسماء الفحوصات اللى أنا بقدر أعملها في هذه
+
+20
+00:01:32,850 --> 00:01:37,690
+الميديا بالنسبة لـ SIM media الـ S يرمز لـ
+
+21
+00:01:37,690 --> 00:01:44,340
+sulphideالـ I يرمز لـ Endol ال M يرمز لـ Motility
+
+22
+00:01:44,340 --> 00:01:48,480
+يبقى بقدر أكشف على ال sulphide على ال endol و على
+
+23
+00:01:48,480 --> 00:01:52,260
+ال motility تعرفنا في المعامل السابقة كيف أنا بدي
+
+24
+00:01:52,260 --> 00:01:56,320
+أقدر أكشف على ال motility و على ال endol لكن ما
+
+25
+00:01:56,320 --> 00:01:59,560
+تعرفنا على ال sulphide اليوم ان شاء الله هنتكلم
+
+26
+00:01:59,560 --> 00:02:05,000
+كيف أنا بدي أكشف على ال H2S production test عن أي
+
+27
+00:02:05,000 --> 00:02:09,820
+أنزين بالظبط بيكشف هذا الفحص شو نواتج الفحصوشو
+
+28
+00:02:09,820 --> 00:02:13,720
+بيعطيه نيلون الاندول والموتيليت اتعرفنا عليه هنمر
+
+29
+00:02:13,720 --> 00:02:17,520
+عليه على السريع بالنسبة لأول فحص اللي هو ال
+
+30
+00:02:17,520 --> 00:02:21,640
+sulfide ال sulfide باكشف على ال H2S production
+
+31
+00:02:21,640 --> 00:02:27,480
+test بدي اكشف على انزايم Sodium Thiosulfate
+
+32
+00:02:27,480 --> 00:02:31,980
+Reductase يبقى من اسمه Sodium Thiosulfate
+
+33
+00:02:31,980 --> 00:02:37,100
+Reductase هيعمل reduction لل Sodium Thiosulfate
+
+34
+00:02:37,510 --> 00:02:40,350
+يبقى ال substrate اللي لازم تكون موجودة في ال
+
+35
+00:02:40,350 --> 00:02:43,750
+media هي Sodium Thiosulfate يبقى هاي ال substrate
+
+36
+00:02:43,750 --> 00:02:48,010
+اللي هيشتغل عليها انزيم Sodium Thiosulfate
+
+37
+00:02:48,010 --> 00:02:54,310
+Reductase هيعمل لها reduction ناتج عملية الاختزال
+
+38
+00:02:54,310 --> 00:02:59,590
+هو ال sulphide بنهاية هيتكون hydrogen sulphide gas
+
+39
+00:02:59,590 --> 00:03:06,090
+اللي هو ال H2S gas هيتكون عندي H2S gasكيف بدي أكشف
+
+40
+00:03:06,090 --> 00:03:12,270
+على الـ H2S Gas؟ قبل إحنا كنا نقول في ال
+
+41
+00:03:12,270 --> 00:03:17,110
+fermentation كان نواتج العملية Gas كنا نضيف نحضر
+
+42
+00:03:17,110 --> 00:03:20,210
+هام إت يوب إذا أتكون air bubble بعرف إنه والله
+
+43
+00:03:20,210 --> 00:03:24,010
+أتكون عندي Gas لكن في هذه ال media هي ال media
+
+44
+00:03:24,010 --> 00:03:30,270
+تعتبر اللي هي فيها نسبة أجاري هي semi-solid بما أن
+
+45
+00:03:30,270 --> 00:03:33,330
+في عندي فحص motility بقى هتكون semi-solid media
+
+46
+00:03:34,210 --> 00:03:37,530
+الفحص ال fermentation كانت ال media broth فبالتالي
+
+47
+00:03:37,530 --> 00:03:41,730
+سهل أني أنا أحط درهام tube لكن في هذا الفحص ما
+
+48
+00:03:41,730 --> 00:03:46,290
+بقدر أحط درهام tube لأنه ال media متصلبة فيها نسبة
+
+49
+00:03:46,290 --> 00:03:49,670
+أجار فبالتالي صعب أني أنا أحط درهام tube فماقدر
+
+50
+00:03:49,670 --> 00:03:54,670
+أكشف على الجاز باستخدام الدرهام tubeكيف بدي اكشف
+
+51
+00:03:54,670 --> 00:04:00,530
+على ال gas ال H2S gas بقوللي بكشف على ال H2S gas
+
+52
+00:04:00,530 --> 00:04:04,910
+بإضافة indicator هذا ال indicator هيرتبط بال H2S
+
+53
+00:04:04,910 --> 00:04:10,390
+gas ويعطيني لون أسود يبقى لو البكتيريا عندها انزيم
+
+54
+00:04:10,740 --> 00:04:15,020
+Sodium Thiosulfate Reductase هتعمل اختزال للـ
+
+55
+00:04:15,020 --> 00:04:17,740
+Substrate اللي هي Sodium Thiosulfate الموجودة في
+
+56
+00:04:17,740 --> 00:04:23,260
+الميديا هينتج H2S Gas كيف بدي أخشف عنه الـ H2S Gas
+
+57
+00:04:23,260 --> 00:04:26,840
+هيربط ب Indicator هذا ال Indicator موجود في
+
+58
+00:04:26,840 --> 00:04:30,640
+الميديا اسمه Ferrous Ammonium Sulfate يبقى هذا هو
+
+59
+00:04:30,640 --> 00:04:35,080
+الـ H2S Indicator هيربط مع الـ H2S Gas لو
+
+60
+00:04:35,080 --> 00:04:38,460
+البكتيريا عندها أنزيم Sodium Thiosulfate Reductase
+
+61
+00:04:38,460 --> 00:04:43,920
+وتكون الـ H2S Gasهيتكون فررس سالفايد هذا المركب
+
+62
+00:04:43,920 --> 00:04:50,160
+black precipitate لونه أسود هيتكون هذا الراسب يبقى
+
+63
+00:04:50,160 --> 00:04:54,060
+لو اتكون عندي لون أسود معناه ان البكتيريا عندها
+
+64
+00:04:54,060 --> 00:04:58,620
+الانزيم كونت غاز اتفاعلت مع اتفاعل الجاز مع ال
+
+65
+00:04:58,620 --> 00:05:01,520
+indicator اللي هو الفررس أمانيوم سالفات ال H2S
+
+66
+00:05:01,520 --> 00:05:05,080
+indicator اللي موجود في ال media و أعطاني اللون
+
+67
+00:05:05,080 --> 00:05:11,250
+الأسودطيب بالنسبة لفحص الـ H2S production قولنا
+
+68
+00:05:11,250 --> 00:05:15,610
+إنه إذا اتكون اللون الأسود يبقى هي هذه positive
+
+69
+00:05:15,610 --> 00:05:19,550
+بتكون فيها انزيم الصادم ثيو سلفات Reductase اعرفنا
+
+70
+00:05:19,550 --> 00:05:24,590
+السبسترات اعرفنا ال enzyme اعرفنا من ال indicator
+
+71
+00:05:24,590 --> 00:05:29,820
+اللي موجود في ال medium طيب كأي فحص كيميائيأكيد
+
+72
+00:05:29,820 --> 00:05:34,200
+البكتيريا بتعمل أو عندها هذا الانزيم بيكون لهدف
+
+73
+00:05:34,200 --> 00:05:38,460
+بيكون فيه فائدة من وجود هذا الانزيم في ال in vivo
+
+74
+00:05:38,460 --> 00:05:41,700
+انا بتكلم عن ان in vivo زي ما قولنا مثلا في فحص
+
+75
+00:05:41,700 --> 00:05:45,960
+الكتلاز كانت تتخلص من ال hydrogen peroxide ال
+
+76
+00:05:45,960 --> 00:05:50,480
+toxic هنا الفائدة من ان البكتيريا تعمل reduction
+
+77
+00:05:50,480 --> 00:05:55,780
+للصلفاتإنه احنا قلنا من ضمن ال inorganic molecule
+
+78
+00:05:55,780 --> 00:05:59,980
+اللي بتستعملها البكتيريا كمستقبل نهائي للإلكترونات
+
+79
+00:05:59,980 --> 00:06:04,120
+في الآن aerobic respiration عشان تنتج الطاقة
+
+80
+00:06:04,120 --> 00:06:09,280
+السلفر يبقى هذا من فوائد هذا الانزيم انه بتعمل
+
+81
+00:06:09,280 --> 00:06:13,310
+reduction للسلفات عشانأنا أستخدم الـ Sulphur
+
+82
+00:06:13,310 --> 00:06:16,150
+كمستقبل نهائي للإلكترونات في الان Europe
+
+83
+00:06:16,150 --> 00:06:20,190
+Prespiration لإنتاج الطاقة طيب خلصنا من أول فحص
+
+84
+00:06:20,190 --> 00:06:25,830
+اللي هو ال H2S Production Testنجي للفحص التاني
+
+85
+00:06:25,830 --> 00:06:30,530
+الاندول طبعا الاندول مر معنا في ال chapter اللي
+
+86
+00:06:30,530 --> 00:06:35,050
+قبل قولنا الاندول بيكشف على أنزيم لتريبتوفاناز
+
+87
+00:06:35,050 --> 00:06:38,970
+لتريبتوفاناز من اسمه هيشتغل على ال substrate
+
+88
+00:06:38,970 --> 00:06:42,770
+لتريبتوفان اللي هي ال amino acid هذي لتريبتوفان
+
+89
+00:06:42,770 --> 00:06:47,450
+بتتكون من اندول وبيوروفات وNH3 مرتبطين ببعض لو
+
+90
+00:06:47,450 --> 00:06:51,770
+أنزيم لتريبتوفاناز موجود في البكتيريا هيكسر
+
+91
+00:06:51,770 --> 00:06:58,590
+لتريبتوفان لاندولpyruvate and NH3 هضيف ال covax
+
+92
+00:06:58,590 --> 00:07:01,950
+reagent بنضيف ال covax reagent هو هذا ال reagent
+
+93
+00:07:01,950 --> 00:07:07,350
+بنضيفه بعد ال incubation بنضيفه على ال media هذا
+
+94
+00:07:07,350 --> 00:07:11,710
+ال covax reagent بيرتبط مع ال indol ناتج من نواتج
+
+95
+00:07:11,710 --> 00:07:16,690
+عملية تكسير للتريبتوفان هيرتبط فيه ويتكون red ring
+
+96
+00:07:16,690 --> 00:07:20,010
+قولنا هاي ال red ring بيكون لونها لون الكرز ال
+
+97
+00:07:20,010 --> 00:07:23,950
+cherry red ringلو اتكونت يبقى هي indol positive
+
+98
+00:07:23,950 --> 00:07:28,010
+بالنسبة لفحص ال motility اتكلمنا انا عشان اشتغل
+
+99
+00:07:28,010 --> 00:07:32,190
+فحص ال motility بزرع البكتيريا في ميديا semi solid
+
+100
+00:07:32,190 --> 00:07:37,170
+media هي الميديا فيها نسبة الأجار 4 من 10% أجار
+
+101
+00:07:37,170 --> 00:07:43,190
+الفكرة انه لو البكتيريا متع المتحركة هلاحظ انه
+
+102
+00:07:43,190 --> 00:07:48,550
+النمو هيكونعلى خط الزراعه وهينتشر بعيدا عن خط
+
+103
+00:07:48,550 --> 00:07:52,350
+الزراعه لكن لو كانت non motile فقط على خط الزراعه
+
+104
+00:07:52,350 --> 00:07:56,790
+هلاقي النمو هذا بالنسبة لفحص ال motility طبعا انا
+
+105
+00:07:56,790 --> 00:08:01,450
+هعمل stabbing يبقى ميديا ال SIM اي ميديا فيها فحص
+
+106
+00:08:01,450 --> 00:08:05,650
+ال motility هتكون الزراعه stabbing هتكون stab tube
+
+107
+00:08:05,650 --> 00:08:08,770
+هزرع stabbing هاخد البكتيريا اللي بدي اكشف عنها
+
+108
+00:08:08,770 --> 00:08:13,830
+هعمل stabbing ل ال media و هعمل incubation لمدة 4
+
+109
+00:08:14,320 --> 00:08:20,760
+بعشرين ساعة هلأ فيها ملاحظتين في هي ال media
+
+110
+00:08:20,760 --> 00:08:25,480
+المحلاحظة الأولى ان انا بعد اربع عشرين ساعة بقرأ
+
+111
+00:08:25,480 --> 00:08:31,880
+نتائج اللي هو فحوصات هي ال media بقولي بقرأ فحص ال
+
+112
+00:08:31,880 --> 00:08:37,300
+motility بعدين بقرأ فحص ال sulfide بعدين بقرأ فحص
+
+113
+00:08:37,300 --> 00:08:42,610
+ال indoor من خلال إضافة ال covax reagent يبقىلازم
+
+114
+00:08:42,610 --> 00:08:48,450
+او ممنوع اقرأ فحص الاندول الا بعد قراءة فحص ال
+
+115
+00:08:48,450 --> 00:08:52,170
+motility why هى يوهان مكتوب بالترتيب بقرأ فحص ال
+
+116
+00:08:52,170 --> 00:08:56,030
+motility بعدين ال H2O production بعدين بضيف ال
+
+117
+00:08:56,030 --> 00:09:00,070
+covax reagent عشان اقرأ ال endol test هيك بالترتيب
+
+118
+00:09:00,070 --> 00:09:03,910
+ممنوع اضيف ال covax reagent و اقرأ ال endol test
+
+119
+00:09:03,910 --> 00:09:09,450
+بعدين اقرأ فحص ال motility هذا سؤال مهم السبب انه
+
+120
+00:09:09,940 --> 00:09:15,040
+الـ COVAX REAGENT قلنا أحد مكوناته الـ HCL ذكرنا
+
+121
+00:09:15,040 --> 00:09:19,260
+سابقاً لما شرحنا فحص الـ Endowel Test بالتفصيل أحد
+
+122
+00:09:19,260 --> 00:09:24,440
+مكونات الـ COVAX REAGENT هو الـ HCL بقول الـ HCL
+
+123
+00:09:24,440 --> 00:09:28,900
+بقلل الـ turbidity الموجودة في الميديا و بيخليها
+
+124
+00:09:28,900 --> 00:09:34,020
+clear بعمل clearance للميدياطيب فحص ال motility
+
+125
+00:09:34,020 --> 00:09:39,000
+بعتمد على ال turbidity انه لو البكتيريا كانت على
+
+126
+00:09:39,000 --> 00:09:42,060
+خط الزراعه نمت على خط الزراعه واتجهت بعيدا عن خط
+
+127
+00:09:42,060 --> 00:09:44,700
+الزراعه بيكون فيها انديه turbidity بقرأ ال
+
+128
+00:09:44,700 --> 00:09:47,600
+turbidity فبعرف ان هي motile لكن لو كانت فقط على
+
+129
+00:09:47,600 --> 00:09:52,220
+خط الزراعه يبقى non motile يبقى قراءة فحص ال
+
+130
+00:09:52,220 --> 00:09:56,020
+motility بعتمد على ال turbidity طب ال Kovacs
+
+131
+00:09:56,020 --> 00:09:59,280
+reagent قلنا ان هو بقلل ال turbidity بعمل
+
+132
+00:09:59,280 --> 00:10:03,790
+clearance ل الميديافبالتالي مش هعرف أقرأ فحص الـ
+
+133
+00:10:03,790 --> 00:10:09,410
+Motility صح لأنه بيعتمد على الـ turbidity عشان هيك
+
+134
+00:10:09,410 --> 00:10:13,690
+بقرأ فحص الـ Motility بشوف هل هي على خط الزراعة
+
+135
+00:10:13,690 --> 00:10:18,490
+فقط يبقى non-motile هل هي طلعت عن خط الزراعة اتجهت
+
+136
+00:10:18,490 --> 00:10:23,350
+بعيدا عن خط الزراعة يبقى هي motile و بعدين بسجل
+
+137
+00:10:23,350 --> 00:10:27,210
+النتيجة و بعدين بضيف ال Covax reagent عشان أقرأ ال
+
+138
+00:10:27,210 --> 00:10:32,950
+Endol testهذا بالنسبة لأول ملاحظة الملاحظة التانية
+
+139
+00:10:32,950 --> 00:10:38,250
+أنه لو البكتيريا H2S positive لو كان عندها انزيم
+
+140
+00:10:38,250 --> 00:10:43,770
+الصيوم ثيوكسلفاتريدكتاز وأنتجت الـ H2S gas ارتبط
+
+141
+00:10:43,770 --> 00:10:47,090
+مع الـ H2S indicator و أعطاني فرر السلفايد اللي
+
+142
+00:10:47,090 --> 00:10:51,950
+يعتبر black precipitate بتكون عندى لون أسود غامق
+
+143
+00:10:51,950 --> 00:10:57,360
+في كل ال mediaطب هل هعرف نتيجة الـ motility لو
+
+144
+00:10:57,360 --> 00:11:00,940
+اتكون لون أسود في ال media أكيد لأ مش هعرف نتيجة
+
+145
+00:11:00,940 --> 00:11:05,220
+ال motility بالتالي لو البكتيريا كانت H2S
+
+146
+00:11:05,220 --> 00:11:10,020
+production positive ال motility ماقدر أتعرف عليها
+
+147
+00:11:10,020 --> 00:11:15,520
+بكتب في نتيجة unknown يبقى لو إجاني سؤالصورة
+
+148
+00:11:15,520 --> 00:11:20,460
+موجودة عليها H2S كان لونها أسود H2S positive فحص
+
+149
+00:11:20,460 --> 00:11:23,800
+الـ motility بنكتب unknown ما بقدر أتعرف على فحص
+
+150
+00:11:23,800 --> 00:11:28,180
+ال motility لأن اللون الأسود غطى على ال media غطى
+
+151
+00:11:28,180 --> 00:11:31,920
+على فحص ال motility وعلى ال turbidity طب إيش الحل
+
+152
+00:11:31,920 --> 00:11:35,940
+عشان أعرف هل البكتيريا motile أو non motile بقولنا
+
+153
+00:11:35,940 --> 00:11:40,760
+بنحضر media خاصة semi solid media فيها نسبة الأجار
+
+154
+00:11:40,760 --> 00:11:46,690
+4 من 10%بدون ما أضيف اللي هو الـ Sodium
+
+155
+00:11:46,690 --> 00:11:50,490
+Thiosulfate الـ Substrate والـ H2S Indicator بدون
+
+156
+00:11:50,490 --> 00:11:54,990
+ما أضيف أي Substrate أو أي Indicator لأي فحوصات
+
+157
+00:11:54,990 --> 00:11:59,410
+تانية زي ما اشتغلنا في أول فحص في الـ Motility
+
+158
+00:11:59,410 --> 00:12:03,510
+كانت هي فقط high and medium nutrient broth مع نسبة
+
+159
+00:12:03,510 --> 00:12:07,990
+أجار 4 من 10%مافيها أي substrate لأي فحوصات
+
+160
+00:12:07,990 --> 00:12:11,810
+كيميائية تانية فهي فقط هتكشف على ال motility هذا
+
+161
+00:12:11,810 --> 00:12:16,250
+بالنسبة للحلقة فكما نكون خلصنا تقريبا أو أجملنا ال
+
+162
+00:12:16,250 --> 00:12:20,430
+SIM media عن شو الفحوصات اللي بتكشف عنها شو
+
+163
+00:12:20,430 --> 00:12:24,150
+النتيجة ال positive و ال negative لكل فحص كيف أنا
+
+164
+00:12:24,150 --> 00:12:29,410
+بدي أعمل هذا الفحص و كيف بدي أقرأ النتائج نيجي لل
+
+165
+00:12:29,410 --> 00:12:34,690
+slidesهي ال media قولنا semi-solid media SIM test
+
+166
+00:12:34,690 --> 00:12:37,910
+medium is a semi-solid media يعني نسبة الأجار فيها
+
+167
+00:12:37,910 --> 00:12:42,590
+4 من 10% قولنا أي ميديا فيها فحص ال motility فهي
+
+168
+00:12:42,590 --> 00:12:47,120
+semi-solid mediaUsed as a differential test medium
+
+169
+00:12:47,120 --> 00:12:51,360
+أكيد بما أنه بتعطيني أو بتفرقلي بكتيريا عن بكتيريا
+
+170
+00:12:51,360 --> 00:12:55,900
+يبقى هي differential test media في أعطت مثلا بعض
+
+171
+00:12:55,900 --> 00:12:58,840
+البكتيريا اللي عندها القدرة على إنتاج الـH2S gas
+
+172
+00:12:58,840 --> 00:13:01,980
+لون أسود لكن البكتيريا اللي ما عندها القدرة إنها
+
+173
+00:13:01,980 --> 00:13:05,200
+تنتج الـH2S ما كان فيه لون أسود يبقى هيعتبرها
+
+174
+00:13:05,200 --> 00:13:08,850
+differential test mediumقلنا الاس بورزموس
+
+175
+00:13:08,850 --> 00:13:13,770
+لاستلفايد من خلال فحص الـH2O production بكشف عن
+
+176
+00:13:13,770 --> 00:13:18,010
+قدرة البكتيريا إنها تستهلك الـ Sodium Thiosulfate
+
+177
+00:13:18,010 --> 00:13:23,130
+قلنا هذا الـsubstrate اللي هيشتغل عليه الإنزيمthey
+
+178
+00:13:23,130 --> 00:13:26,110
+can reduce it to sulfide using the enzyme
+
+179
+00:13:26,110 --> 00:13:31,630
+thiosulfate reductase قولنا من خلال انزيم ال
+
+180
+00:13:31,630 --> 00:13:34,210
+thiosulfate reductase أو ال sodium thiosulfate
+
+181
+00:13:34,210 --> 00:13:38,710
+reductase اللي موجود في بعض أنواع البكتيريا هتعمله
+
+182
+00:13:38,710 --> 00:13:41,850
+البكتيريا reduction لل sodium thiosulfate
+
+183
+00:13:41,850 --> 00:13:45,110
+السبسترات الموجود في الميديا هينتج بالنهاية
+
+184
+00:13:45,110 --> 00:13:49,770
+sulfite او هينتج بالنهاية هينتج sulfite هتعمله
+
+185
+00:13:49,770 --> 00:13:53,560
+reduction و بالنهاية هيتكونالـ hydrogen sulphide
+
+186
+00:13:53,560 --> 00:13:59,000
+gas بالنسبة للـ I الـ I قلنا هي بتكشف عن الاندول
+
+187
+00:13:59,000 --> 00:14:04,280
+باكشف عن قدرة البكتيريا انها تنتج لـ tryptophanase
+
+188
+00:14:04,280 --> 00:14:07,620
+قلنا أنزيم لـ tryptophanase بيكسر ال amino acid
+
+189
+00:14:07,620 --> 00:14:11,840
+الـ tryptophan لاندول امانيام and pyruvic acid ال
+
+190
+00:14:11,840 --> 00:14:15,140
+motility بدي اكشف على ال motility ال motility هو
+
+191
+00:14:15,140 --> 00:14:20,570
+وجود الفلاجلةهل البكتيريا بتقدر تسبح خلال ال media
+
+192
+00:14:20,570 --> 00:14:25,450
+هل بتقدر تتجه بعيدا عن خط الزراعة ولا لأ مكونات ال
+
+193
+00:14:25,450 --> 00:14:30,250
+media نسبة الأجار 4 من 10% أجار هذا لفحص مهم جدا
+
+194
+00:14:30,250 --> 00:14:33,830
+لفحص ال mortality عشان هيك ال media تعتبر semi
+
+195
+00:14:33,830 --> 00:14:37,850
+solid media بيبتون البيبتون هو عبارة عن قلنا
+
+196
+00:14:37,850 --> 00:14:41,700
+مجموعة من ال amino acid مرتبطة مع بعضها البعضwhich
+
+197
+00:14:41,700 --> 00:14:45,180
+is rich in tryptophan amino acid يبقى هو موجود فيه
+
+198
+00:14:45,180 --> 00:14:48,880
+هو ال tryptophan اللي يعتبر substrate مهم لفحص
+
+199
+00:14:48,880 --> 00:14:52,900
+الإندول الصواتيومثيو سلفات قلنا substrate لفحص ال
+
+200
+00:14:52,900 --> 00:14:57,360
+H2S production يبقى هدول التلاتة substrate لكل فحص
+
+201
+00:14:57,360 --> 00:15:01,960
+من الفحوصات التلاتة اللي بتكشف عنهم ميديا ال SIM
+
+202
+00:15:01,960 --> 00:15:06,160
+في الراس أمانيوم سلفات قلنا هو ال H2S indicator
+
+203
+00:15:06,160 --> 00:15:11,520
+اللي هيكشف على وجود ال H2S gasهذه الميديا SIM
+
+204
+00:15:11,520 --> 00:15:16,720
+بتكشف على تلت أنشطة بتقوم بيها الـ Enteric
+
+205
+00:15:16,720 --> 00:15:21,740
+Bacteria Enteric Bacteria بتفرق لي بعنهم أول إيش
+
+206
+00:15:21,740 --> 00:15:24,620
+قلنا اللي هو فحص السلفات Sodium Thiosulfate
+
+207
+00:15:24,620 --> 00:15:27,240
+السبسترات اللي هيشتغل عليها أنزيم Sodium
+
+208
+00:15:27,240 --> 00:15:30,460
+Thiosulfate Reductase First Ammonium Sulfate هو
+
+209
+00:15:30,460 --> 00:15:34,540
+الـH2S Indicatorعشان أكشف على الـ hydrogen sulfide
+
+210
+00:15:34,540 --> 00:15:38,000
+production اللي هو الـ H2S production قلنا
+
+211
+00:15:38,000 --> 00:15:40,240
+الferrous ammonium sulfate اللي هو الـ H2S
+
+212
+00:15:40,240 --> 00:15:44,200
+indicator بيرتبط مع الـ H2S gas اللي ينتج من عملية
+
+213
+00:15:44,200 --> 00:15:48,100
+ال reduction عشان ينتج بالنهاية الferrous sulfide
+
+214
+00:15:48,100 --> 00:15:53,290
+هذا المركب لونه أسود black precipitateتانى نشاط
+
+215
+00:15:53,290 --> 00:15:57,490
+بتكشف عنه هذه ال media اللى هو الفحص الاندول
+
+216
+00:15:57,490 --> 00:16:03,070
+البيبتون موجود is rich in tryptophan بقولنا انه
+
+217
+00:16:03,070 --> 00:16:07,990
+البيبتون بيحتوي على ال tryptophan بدي اكشف هل
+
+218
+00:16:07,990 --> 00:16:12,590
+البكتيريا بتقدر انها تهاجم هذا ال tryptophan تكسر
+
+219
+00:16:12,590 --> 00:16:16,730
+ال tryptophan ناتجة عملية التكسير هيتكون عندى
+
+220
+00:16:16,730 --> 00:16:20,930
+اندول كيف بكشف على الاندول بضيف ال covax reagent
+
+221
+00:16:21,790 --> 00:16:25,710
+فحص الـ motility فحص الـ motility أكيد بقدر أني
+
+222
+00:16:25,710 --> 00:16:29,150
+أنا أكشف عنه في هذه ال media نتيجة الـ semi-solid
+
+223
+00:16:29,150 --> 00:16:32,030
+nature of the media نتيجة أنه ال media هي semi
+
+224
+00:16:32,030 --> 00:16:35,310
+-solid فبالتالي لو البكتيريا عندها القدرة أنها
+
+225
+00:16:35,310 --> 00:16:39,310
+تنمو radiating out from the center step line لو
+
+226
+00:16:39,310 --> 00:16:43,190
+قدر تنمو بعيدا عن خط الزراعي يبقى هذا يشير أنه
+
+227
+00:16:43,190 --> 00:16:48,320
+البكتيريا أو test organism is motileبالنسبة لـ
+
+228
+00:16:48,320 --> 00:16:53,340
+Significant لهذا الفحص قلنا أي فحص بيكون في يد انا
+
+229
+00:16:53,340 --> 00:16:56,500
+بيكون لإله Significant أهمية عشان يفرق بين مين
+
+230
+00:16:56,500 --> 00:17:00,000
+ومين فحص الـ Motility اتكلمنا عنه لما شرحنا على
+
+231
+00:17:00,000 --> 00:17:02,820
+البكتيريا الـ Motility و الـ Indoor لما شرحناهم
+
+232
+00:17:02,820 --> 00:17:07,880
+بالتفصيللكن بالنسبة للـ H2S production الـ H2S
+
+233
+00:17:07,880 --> 00:17:12,060
+production بقول لي إنه الـ significant لهذا الفحص
+
+234
+00:17:12,060 --> 00:17:15,940
+the ability to reduce sulfur is specific to two
+
+235
+00:17:15,940 --> 00:17:19,140
+members of the enteric family Salmonella and
+
+236
+00:17:19,140 --> 00:17:24,680
+Brutias بقول لي إن قدرة البكتيريا إنها تعمل اختصار
+
+237
+00:17:24,680 --> 00:17:30,660
+للسلفر فقط بكتيريتين تنتين هم اللي H2S production
+
+238
+00:17:30,660 --> 00:17:34,140
+positiveمن الـ Enteric family اللي هي عائلة الـ
+
+239
+00:17:34,140 --> 00:17:38,520
+Enterobacteriaceae السلمونيلا و البروتياس التانتان
+
+240
+00:17:38,520 --> 00:17:43,940
+هدول H2S positive احنا قلنا اذا بتتذكروا بفحص
+
+241
+00:17:43,940 --> 00:17:49,520
+البروتياس بفحص اليورياز sorry بفحص اليورياز قلنا
+
+242
+00:17:49,520 --> 00:17:54,400
+بيفرق بين ال non-lactose fermenter اللي هم
+
+243
+00:17:54,400 --> 00:17:58,600
+السلمونيلا شجلة البروتياس هدولة التلاتة يعتبروا
+
+244
+00:17:58,600 --> 00:18:02,930
+non-lactose fermenterموجودين في عينات ال stone
+
+245
+00:18:02,930 --> 00:18:07,370
+قلنا عشان افرق بينهم بعمل فحص اليورياز لو طلعت
+
+246
+00:18:07,370 --> 00:18:11,670
+يورياز positive يبقى بروتياس لو طلعت يورياز
+
+247
+00:18:11,670 --> 00:18:16,690
+negative يبقى سلمونيلا او شجلة طيب السلمونيلا و
+
+248
+00:18:16,690 --> 00:18:21,590
+الشجلة كيف بدي افرق بينهم؟بقول من خلال فحص ال
+
+249
+00:18:21,590 --> 00:18:27,450
+motility و ال H2S production السلمونيلا H2S
+
+250
+00:18:27,450 --> 00:18:32,390
+production positive و motile لكن شجلة non motile و
+
+251
+00:18:32,390 --> 00:18:38,470
+non H2S او تعتبر H2S production negative البروتياس
+
+252
+00:18:38,470 --> 00:18:43,310
+نفس السلمونيلا motile زاد H2S positive لكن كيف
+
+253
+00:18:43,310 --> 00:18:46,830
+بفرق بينهم من خلال فحص اليوريايبقى هي مكتوب
+
+254
+00:18:46,830 --> 00:18:51,650
+السلمونيلا H2S positive and motile لبروتياز H2S
+
+255
+00:18:51,650 --> 00:18:54,810
+positive and motile كيف بفرق بينهم من خلال اتكلمنا
+
+256
+00:18:54,810 --> 00:18:57,950
+عنه هذا الاشي سابقا من خلال فحص اليورياز
+
+257
+00:18:57,950 --> 00:19:03,370
+السلمونيلا يورياز negative لبروتياز positive
+
+258
+00:19:03,370 --> 00:19:10,470
+الشجلة عكس السلمونيلا و لبروتياز H2S negative and
+
+259
+00:19:10,470 --> 00:19:15,280
+non motile لكن هي يورياز negativeالـ Eclipsella
+
+260
+00:19:15,280 --> 00:19:20,480
+اتفقنا إنه هي فقط الوحيدة الـ non-motile من
+
+261
+00:19:20,480 --> 00:19:25,900
+الكوليفارم and doesn't produce H2S الـ E.coli
+
+262
+00:19:25,900 --> 00:19:31,320
+تعتبر motile وكمان doesn't produce H2S قلنا في
+
+263
+00:19:31,320 --> 00:19:35,080
+عندي فحص اللي هو ال MVEC بقدر أفرق بين ال E.coli و
+
+264
+00:19:35,080 --> 00:19:39,360
+ال E.clipsella ال MVEC اللي هي بفرق بين الكوليفارم
+
+265
+00:19:39,360 --> 00:19:45,530
+حتى ككل مش بس ال E.clipsella و E.coliكيف أنا بدي
+
+266
+00:19:45,530 --> 00:19:49,050
+أشغل هذا الفحص أكيد بعمل label للي تيوب الـ SIM
+
+267
+00:19:49,050 --> 00:19:53,810
+أجار دي بتيوب كيف بتكون هي أجار من أشكال من أشكال
+
+268
+00:19:53,810 --> 00:19:58,510
+اللي هو ال media في ال SIM بتكون أجار دي بتيوب
+
+269
+00:19:58,510 --> 00:20:03,270
+بعمل label باسم البكتيريا اللي بدي أكشف عنها و
+
+270
+00:20:03,270 --> 00:20:09,790
+بكتب طبعا اللي هو تاريخ الزراعةبعمل طبعا ال
+
+271
+00:20:09,790 --> 00:20:12,770
+stabbing بستخدم ال aseptic technique بمنع أي كنت
+
+272
+00:20:12,770 --> 00:20:17,950
+ماناشي انه سير بعمل ال stabbing ل ال media بحاول
+
+273
+00:20:17,950 --> 00:20:21,310
+ان انا ابعد على ال bottom of the tube اخر ال tube
+
+274
+00:20:21,310 --> 00:20:27,610
+ما بعمل ال stabbing له تقريبا ببعد شوية مسافةبعمل
+
+275
+00:20:27,610 --> 00:20:32,950
+incubation لمدة 24 ساعة درجة حرارة 37 هنكتب
+
+276
+00:20:32,950 --> 00:20:36,230
+لملاحظة قولناها سابقا ان لو البكتيري عندها القدرة
+
+277
+00:20:36,230 --> 00:20:40,190
+انها تنتج ال hydrogen sulfide يبقى بالنهاية هيتكون
+
+278
+00:20:40,190 --> 00:20:43,990
+black precipitate فبالتالي مش هقدر اكشف على ال
+
+279
+00:20:43,990 --> 00:20:48,410
+motility of organismعشان هيك بفكر أن فحص ال
+
+280
+00:20:48,410 --> 00:20:53,150
+motility هو false negative result مش هيبين عندي مش
+
+281
+00:20:53,150 --> 00:20:56,970
+هتبين الحركة فبالتالي هفكرها negative لكن هي false
+
+282
+00:20:56,970 --> 00:21:02,190
+negative resultبالـ H2S Production اللون الأسود
+
+283
+00:21:02,190 --> 00:21:05,850
+اللي اتكون هو اللي خلاني ما أشوف الـ Turbidity
+
+284
+00:21:05,850 --> 00:21:10,870
+فبالتالي أحدد الـ Motility للبكتيريا عشان هيك الحل
+
+285
+00:21:10,870 --> 00:21:15,970
+أني أنا بعمل Semi Solid Media بدون ما يكون فيه
+
+286
+00:21:15,970 --> 00:21:19,010
+اندي Substrate اللي هي الـ Sodium Thiosulfate أو
+
+287
+00:21:19,010 --> 00:21:22,810
+الـ H2S Indicator فقط بتتكون من الـ Nutrient
+
+288
+00:21:22,810 --> 00:21:33,130
+Growth مع نسبة أجار 4 من 10%بالنسبة للنتائج هي
+
+289
+00:21:33,130 --> 00:21:37,940
+الصورتان نفس الشيءأول شئ هنقرأ فحص الـ Motility
+
+290
+00:21:37,940 --> 00:21:42,120
+أكيد اللي بالنسبة لـ Motility هي الـ bacteria موطة
+
+291
+00:21:42,120 --> 00:21:46,080
+اللي بنلاحظ أنه في عندي بعيدا عن خط الزراعة في
+
+292
+00:21:46,080 --> 00:21:50,860
+عندي turbidity بعدها هقرأ الـ edge to us هي
+
+293
+00:21:50,860 --> 00:21:55,120
+negative edge to us negative فحص ال Indol دفت
+
+294
+00:21:55,120 --> 00:21:58,980
+الكوفاكس رياجانت ماتحول اللون للون أحمر في أعلى
+
+295
+00:21:58,980 --> 00:22:03,080
+الحلقة يبقى هي في أعلى ال tube يبقى هي Indol
+
+296
+00:22:03,080 --> 00:22:08,130
+negativeهان بالنسبة لفحص الـ Motility من اللحظة
+
+297
+00:22:08,130 --> 00:22:13,070
+أنه على خط الزراعي يبقى هي non-motile فحص الـ H2S
+
+298
+00:22:13,070 --> 00:22:17,150
+نيجاتيف فحص الاندول positive اتكوّن لون cherry red
+
+299
+00:22:17,150 --> 00:22:24,250
+يبقى هي positive هان H2S positive ماقدر اكشف على
+
+300
+00:22:24,250 --> 00:22:28,030
+ال motility فبكتب unknown ال motility بكتب عنها
+
+301
+00:22:28,030 --> 00:22:31,710
+unknown بالنسبة لالاندول negative
+
+302
+00:22:34,450 --> 00:22:39,650
+هذه الـ tube هي ك control مش مزروعة هذه ال tube
+
+303
+00:22:39,650 --> 00:22:43,890
+نقرأ أول إشي نتيجة ال motility فقط هي هنا على خط
+
+304
+00:22:43,890 --> 00:22:49,330
+الزراعية هي يبقى هي non motile بالنسبة ل ال H2S
+
+305
+00:22:49,330 --> 00:22:52,950
+negative بالنسبة ل ال indol من اللاحظ في عندي لون
+
+306
+00:22:52,950 --> 00:22:58,530
+أحمر يبقى هي indol positive هنا ال H2S positive
+
+307
+00:22:58,530 --> 00:23:02,780
+طبعا ال motility unknownبالنسبة للاندول positive
+
+308
+00:23:02,780 --> 00:23:06,880
+هان ال H2S positive بالنسبة للموتيليتي unknown
+
+309
+00:23:06,880 --> 00:23:10,500
+بالنسبة للاندول negative يبقى هيك نقرأ النتيجة
+
+310
+00:23:10,500 --> 00:23:16,380
+بنكتب التلت فحوصات SIM عشان ما نطلع خبطش نكتب S
+
+311
+00:23:16,380 --> 00:23:21,090
+اللي هو لسه الفايد I للاندول M للموتيليتيبنقرأ أول
+
+312
+00:23:21,090 --> 00:23:24,210
+issue ال motility بعدين ال S2S بعدين بنضيف ال
+
+313
+00:23:24,210 --> 00:23:27,910
+Govex reagent وبنقرأ نتيجة ال indoor هيك بنكون
+
+314
+00:23:27,910 --> 00:23:34,430
+خلصنا أول media اللي هي ال SIM media نيجي لتاني
+
+315
+00:23:34,430 --> 00:23:39,150
+media تعتبر single media multiple test اللي هي ل
+
+316
+00:23:39,150 --> 00:23:44,970
+triple sugar iron agar or clicar iron agarهدولا
+
+317
+00:23:44,970 --> 00:23:49,950
+الميديتين نفس ال media لكن الاختلاف انه ال kliger
+
+318
+00:23:49,950 --> 00:23:55,650
+iron agar بتحتوي على نوعين من أنواع السكر اللي هم
+
+319
+00:23:55,650 --> 00:24:00,410
+ال dextrose اللي هو الجليكوز زائد اللاكتوز لكن ال
+
+320
+00:24:00,410 --> 00:24:04,230
+triple sugar iron agar بتحتوي على ثلاث أنواع من
+
+321
+00:24:04,230 --> 00:24:09,500
+السكريات من اسمها tripledextrose زي الكليجر زائد
+
+322
+00:24:09,500 --> 00:24:13,080
+lactose برضه زي الكليجر لكن الإضافة المختلفة عن
+
+323
+00:24:13,080 --> 00:24:17,160
+الكليجر اللي هي الساكروز يبقى اتنتاين نفس الاشي
+
+324
+00:24:17,160 --> 00:24:22,880
+لكن الفرق انه سكر الساكروز مش موجود في الكليجر
+
+325
+00:24:22,880 --> 00:24:27,580
+iron agar فقط موجود في ال triple sugar iron agar
+
+326
+00:24:27,580 --> 00:24:33,690
+هي ال media من خلالها بكشف علىدي اميناشن للبيبتون
+
+327
+00:24:33,690 --> 00:24:38,430
+ال fermentation وال H2S production ففي هذه ال
+
+328
+00:24:38,430 --> 00:24:44,810
+media منصبها في slant slant هي بتكون slant اتيوب
+
+329
+00:24:44,810 --> 00:24:50,310
+ليش انا صبيتها في slant اتيوب عشان اوفر ظروف
+
+330
+00:24:50,310 --> 00:24:54,810
+هوائية aerobic condition من خلال ال slant سطح ال
+
+331
+00:24:54,810 --> 00:24:58,680
+slant هذا موفر aerobic conditionوهذا الـ
+
+332
+00:24:58,680 --> 00:25:01,580
+deamination اللي اللي بيبتوع بيحصل في ظروف aerobic
+
+333
+00:25:01,580 --> 00:25:07,060
+condition كمان بدي أوفر من خلال البط او اللي تحت
+
+334
+00:25:07,060 --> 00:25:10,140
+ال slant هذا بسميه بط اللي بكون موجود تحت هذا
+
+335
+00:25:10,140 --> 00:25:13,920
+بسميه بط هذا بوفر ظروف an aerobic condition ظروف
+
+336
+00:25:13,920 --> 00:25:17,620
+لا هوائية ال fermentation و القشط و أسي production
+
+337
+00:25:17,620 --> 00:25:23,030
+بيحصلوا في ظروف لا هوائية عشان هيك بديأنا أوفر
+
+338
+00:25:23,030 --> 00:25:27,710
+ظروف هوائية وظروف لا هوائية فبصب ال media ب slant
+
+339
+00:25:27,710 --> 00:25:30,730
+لأن ال-d-amination للبيبتون بيصير في ظروف European
+
+340
+00:25:30,730 --> 00:25:33,710
+condition ال fermentation و ال-H2S production
+
+341
+00:25:33,710 --> 00:25:38,410
+بيصير في ظروف Un-European condition يبقى ال slant
+
+342
+00:25:38,410 --> 00:25:41,930
+هكشف على ال-d-amination للبيبتون ال-but هكشف على
+
+343
+00:25:41,930 --> 00:25:45,990
+ال fermentation و ال-H2S production في high ال
+
+344
+00:25:45,990 --> 00:25:50,800
+media في عندي BH indicatorلش لأنه نواتج عملية ال
+
+345
+00:25:50,800 --> 00:25:54,280
+fermentation acid و نواتج عملية ال deamination
+
+346
+00:25:54,280 --> 00:25:57,400
+للبيبتون في عندي alkaline product فبالتالي بما أنه
+
+347
+00:25:57,400 --> 00:26:00,680
+اتكون alkaline او ال acidic product هضيف BH
+
+348
+00:26:00,680 --> 00:26:05,280
+Indicator عشان استدل على حدوث ال fermentation او
+
+349
+00:26:05,280 --> 00:26:09,940
+ال deamination للبيبتون ال BH Indicator الموجود في
+
+350
+00:26:09,940 --> 00:26:13,600
+ال media هو ال phenol red يعني ال phenol red مر
+
+351
+00:26:13,600 --> 00:26:17,400
+معنى للمرة التالتة اول مرة مر في فحص ال
+
+352
+00:26:17,400 --> 00:26:21,370
+fermentationتاني مرة في فحص اليوريا تالت مرة في
+
+353
+00:26:21,370 --> 00:26:25,530
+فحص يوليس ريبيل شوجر ايرون اجار حدوده في ال acid
+
+354
+00:26:25,530 --> 00:26:28,990
+بيكون لونه yellow في ال alkaline بيكون لونه red في
+
+355
+00:26:28,990 --> 00:26:32,990
+ال neutral بيكون لونه orange بما اني انا بدي اكشف
+
+356
+00:26:32,990 --> 00:26:37,150
+على H2S production يبقى اكيد لازم يكون موجود في ال
+
+357
+00:26:37,150 --> 00:26:42,510
+media H2S indicator زي فحص ال SIM لكن ال H2S
+
+358
+00:26:42,510 --> 00:26:47,920
+indicator هو فرق امونيوم سيترات تمامفرق أمانيا
+
+359
+00:26:47,920 --> 00:26:51,000
+المسيطرات اللى موجود في هذه ال media ال H2S
+
+360
+00:26:51,000 --> 00:26:56,100
+Indicator طيب لو البكتيريا عملت gelicose
+
+361
+00:26:56,100 --> 00:26:59,120
+fermentation يبقى هيتكون ناتجة عملية ال
+
+362
+00:26:59,120 --> 00:27:04,580
+fermentation acid هيتحول لون البطل للون yellow صح
+
+363
+00:27:04,580 --> 00:27:09,220
+لكن لو البكتيريا عملت deamination للبيبطون هتنزع
+
+364
+00:27:09,220 --> 00:27:14,540
+الأمانيا البيبطون عبارة عن قلنا amino acidالأمانو
+
+365
+00:27:14,540 --> 00:27:17,960
+أسد الهيكلية تبعته في يندي أمونيا عملت دي
+
+366
+00:27:17,960 --> 00:27:21,340
+emination زلة هاي الأمونيا يبقى الأمونيا عندى
+
+367
+00:27:21,340 --> 00:27:26,360
+alkaline product هترفع ال pH هيتكون اللون هيتحول
+
+368
+00:27:26,360 --> 00:27:30,170
+لون ال media للون أحمرلو البكتيريا عندها القدرة
+
+369
+00:27:30,170 --> 00:27:34,990
+انها تعمل H2S production قلنا هيتكون H2S gas
+
+370
+00:27:34,990 --> 00:27:37,950
+هيرتبط مع ال indicator الموجود في ال media هيتكون
+
+371
+00:27:37,950 --> 00:27:41,650
+عندنا black precipitate في البط يبقى هك باعرف انه
+
+372
+00:27:41,650 --> 00:27:48,750
+صار فيه عندنا H2S production هذا بالنسبة لكف او اش
+
+373
+00:27:48,750 --> 00:27:53,650
+اللي بيصير في هاي ال media هلأ لو صار فيه عندنا او
+
+374
+00:27:53,650 --> 00:27:57,070
+اتكون لون يلو في البط برمز ليلو بالرمز A
+
+375
+00:28:06,490 --> 00:28:11,530
+لو أتكون لون أحمر في ال slant صار في عندي
+
+376
+00:28:11,530 --> 00:28:20,130
+deamination للبيبتون بارمز بالرمز K هنفهم
+
+377
+00:28:20,130 --> 00:28:25,430
+أكتر لما نقرأ النتائج أو نكتب النتيجة ال black
+
+378
+00:28:25,430 --> 00:28:27,630
+abrasive بتاعي بكتب H2S
+
+379
+00:28:35,780 --> 00:28:40,500
+لون ال media بيكون لون orange تمام فبالتالي لو
+
+380
+00:28:40,500 --> 00:28:44,200
+اتحول اللون للون يلو في ال pot يبقى هكذا لو صار في
+
+381
+00:28:44,200 --> 00:28:48,440
+عندي gelicose fermentation لو صار في عندي لون أحمر
+
+382
+00:28:48,440 --> 00:28:51,360
+في ال slant يبقى صار في عندي deamination للبيبطان
+
+383
+00:28:51,360 --> 00:28:55,500
+لو اتكون black precipitate معناه ان في عندي H2S
+
+384
+00:28:55,500 --> 00:28:56,060
+production
+
+385
+00:29:02,120 --> 00:29:08,300
+هلأ بالنسبة لكيف بيصير او كيف البكتيريا بتستهلك
+
+386
+00:29:08,300 --> 00:29:13,040
+المواد الغذائية هلأ كل البكتيريا اللى عندها القدرة
+
+387
+00:29:13,040 --> 00:29:16,820
+انها تعمل fermentation هتعمل fermentation لأبسط
+
+388
+00:29:16,820 --> 00:29:21,920
+سكر اللى هو الجليكوز يبقى روحت أنا زراعة البكتيريا
+
+389
+00:29:21,920 --> 00:29:26,820
+في high ال media أول مصدر رح تلجأ لإله البكتيريا
+
+390
+00:29:26,820 --> 00:29:31,020
+هو الجليكوزالموجود في ال media أكيد هيكون فيه
+
+391
+00:29:31,020 --> 00:29:34,280
+جليكوز بما أنني بدي أكشف على ال fermentation هيكون
+
+392
+00:29:34,280 --> 00:29:38,960
+موجود جليكوز لكن موجود بكمية قليلة يبقى أول مصدر
+
+393
+00:29:38,960 --> 00:29:44,640
+البكتيريا هتروح له هو الجليكوز لو البكتيريا عندها
+
+394
+00:29:44,640 --> 00:29:49,540
+القدرة أنها تعمل fermentation هتروح لجليكوز و تعمل
+
+395
+00:29:49,540 --> 00:29:53,730
+fermentation يبقى ال fermentation قلنالو عندها
+
+396
+00:29:53,730 --> 00:29:56,530
+البكتيريا القدرة انها تعمل fermentation هتروح
+
+397
+00:29:56,530 --> 00:30:01,370
+لأبسط سكر أول مرحلة خلال أول 12 ساعة هتروح
+
+398
+00:30:01,370 --> 00:30:06,450
+للجليكوز اللي هو أبسط سكرهتعمله fermentation يبقى
+
+399
+00:30:06,450 --> 00:30:11,050
+أول 12 ساعة هتلجأ البكتيريا للجليكوز وهتعمله تخمير
+
+400
+00:30:11,050 --> 00:30:18,670
+هيتكون acid حمض هيرمل على خفض البيئة هيتحول لون ال
+
+401
+00:30:18,670 --> 00:30:22,250
+media للون أصفر في البط قولنا ال fermentation
+
+402
+00:30:22,250 --> 00:30:24,950
+بيحصل في ال and European condition يبقى بيحصل في
+
+403
+00:30:24,950 --> 00:30:31,260
+البط هيتحول لون ال tube للون أصفر في البطلكن سعر
+
+404
+00:30:31,260 --> 00:30:34,060
+هو ال fermentation في الـun-aerobic condition لكن
+
+405
+00:30:34,060 --> 00:30:39,220
+اللون الأصفر بيمتد في كل tube سواء في البط أو سواء
+
+406
+00:30:39,220 --> 00:30:43,180
+في الإسلان يبقى ال fermentation في سير في ظروف لا
+
+407
+00:30:43,180 --> 00:30:47,580
+هوائية بيحول بما أن سعر ال fermentation هيتكون
+
+408
+00:30:47,580 --> 00:30:51,440
+acid ال acid هيعمل على خفض ال pH هيتحول لون ال
+
+409
+00:30:51,440 --> 00:30:55,360
+media للون ال tube هيمتد اللون الأصفر في كل tube
+
+410
+00:30:55,360 --> 00:31:00,440
+سواء في البط أو في الإسلانيبقى برمز لهذه النتيجة
+
+411
+00:31:00,440 --> 00:31:04,880
+على بما أنه هنتكوّن لون يلو و هان لون يلو يبقى A
+
+412
+00:31:04,880 --> 00:31:09,100
+يلو فوق بالإسلانت على A في البط يبقى A يلو فوق
+
+413
+00:31:09,100 --> 00:31:09,240
+بالإسلانت على A في البط يبقى A يلو فوق بالإسلانت
+
+414
+00:31:09,240 --> 00:31:12,620
+على A في البط يبقى A يلو فوق بالإسلانت على A في
+
+415
+00:31:12,620 --> 00:31:17,020
+البط يبقى A يلو فوق بالإسلانت على A في البط يبقى A
+
+416
+00:31:17,020 --> 00:31:20,020
+يلو فوق بالإسلانت على A في البط يبقى A يلو فوق
+
+417
+00:31:20,020 --> 00:31:21,160
+بالإسلانت على A في البط يبقى A يلو فوق بالإسلانت
+
+418
+00:31:21,160 --> 00:31:25,160
+على A في البط يبقى A يلو فوق بالإسلانت على A
+
+419
+00:31:26,620 --> 00:31:30,700
+الجليكوز قُلت موجود بكمية قليلة في الميديا موجود
+
+420
+00:31:30,700 --> 00:31:36,180
+بنسبة 1 من 10% بعد ما يخلص الجليكوز أكيد البكتيريا
+
+421
+00:31:36,180 --> 00:31:40,880
+هتروح لمصادر تانية موجود في الميديا قلنا لل triple
+
+422
+00:31:40,880 --> 00:31:45,880
+والكليجر iron agar قلنا موجود جليكوز وموجود
+
+423
+00:31:45,880 --> 00:31:51,190
+lactose فبالتالي البكتيريا هتروح لل lactoseلو
+
+424
+00:31:51,190 --> 00:31:54,370
+البكتيريا هذا خلال أول 12 ساعة قلنا البكتيريا
+
+425
+00:31:54,370 --> 00:31:58,110
+بتروح للجليكوز موجود بكمية قليلة هتروح بعدين
+
+426
+00:31:58,110 --> 00:32:03,150
+لمصادر تانية بقولي من خلال بعد 12 ل 24 ساعة
+
+427
+00:32:03,150 --> 00:32:07,270
+البكتيريا هتروح للكتوز لو البكتيريا عندها القدرة
+
+428
+00:32:07,270 --> 00:32:12,030
+انها تخمر اللكتوز او الساكراز لو ال media لو ال
+
+429
+00:32:12,030 --> 00:32:14,770
+media هي ال triple sugar iron agar لو البكتيريا
+
+430
+00:32:14,770 --> 00:32:18,150
+عندها القدرة انها تعمل fermentation لالهم ايش
+
+431
+00:32:18,150 --> 00:32:25,670
+هيصير؟هتعمل تخمير لباقي السكريات هينتج جمية كبيرة
+
+432
+00:32:25,670 --> 00:32:30,990
+من ال acid من الحمض هيعمل على طبعا خفض ال pH راح
+
+433
+00:32:30,990 --> 00:32:34,330
+يظل كل tube لونه أصفر يبقى هتظلها النتيجة نفس
+
+434
+00:32:34,330 --> 00:32:37,510
+الشيء yellow في ال slant و yellow في ال butt يبقى
+
+435
+00:32:37,510 --> 00:32:42,070
+النتيجة a على a a على اللي هي ال slant على a في ال
+
+436
+00:32:42,070 --> 00:32:46,650
+butt يبقى هيتكون جمية acid بصورة كبيرة هيظل ال
+
+437
+00:32:46,650 --> 00:32:50,650
+tube كله لونه أصفرطب لو البكتيريا ما عندها القدرة
+
+438
+00:32:50,650 --> 00:32:55,410
+على تخمير باقي السكريات زي اللاكتوز او السكاروز
+
+439
+00:32:55,410 --> 00:33:00,250
+اكيد هتلجأ لمصادر اخرى احنا قلنا اولا الجليكوز
+
+440
+00:33:00,250 --> 00:33:05,910
+بعدين اللاكتوز او السكاروز مش موجودين هتروح لمصادر
+
+441
+00:33:05,910 --> 00:33:09,310
+اخرى المصدر اللى بعده اللى هتروح البكتيريا عليه
+
+442
+00:33:09,310 --> 00:33:14,560
+اللى هو البيبطان البيبطان موجود في ال mediaهتعمله
+
+443
+00:33:14,560 --> 00:33:17,920
+الـ deamination للبيبتون قولنا هيتكون الأمانيا
+
+444
+00:33:17,920 --> 00:33:21,720
+الأمانيا alkaline product ال alkaline product
+
+445
+00:33:21,720 --> 00:33:26,320
+هيتحول نتيجة اللي هو وجود الفيه نار red للون لون
+
+446
+00:33:26,320 --> 00:33:30,880
+ال media هيتحول للون red في ال slant فقط في ال
+
+447
+00:33:30,880 --> 00:33:36,580
+slant قولنا هيتحول لون ال media للون أحمر لأنه
+
+448
+00:33:36,580 --> 00:33:40,800
+احنا قولنا ان ال deamination للبيبتون هذا هيصير
+
+449
+00:33:40,800 --> 00:33:46,700
+فقط فيظروف هوائية في الـ aerobic condition فقط
+
+450
+00:33:46,700 --> 00:33:49,780
+هيصير في الـ slant يبقى هيتحول لون ال slant فقط
+
+451
+00:33:49,780 --> 00:33:55,040
+للون red لكن ال butt هيظل لونه أصفر يبقى هان نحكي
+
+452
+00:33:55,040 --> 00:34:00,120
+لون red أحمر و لون ال butt لون ال butt هيظل لون
+
+453
+00:34:00,120 --> 00:34:05,660
+أصفر يبقى red على yellow red قلنا نرمز لإله بالرمز
+
+454
+00:34:05,660 --> 00:34:11,910
+K يبقى K على A هيك نرمز النتيجة K على Aهذا بالنسبة
+
+455
+00:34:11,910 --> 00:34:16,170
+لو البكتيريا هيك قرأت او هيك الفحص هيك النتيجة
+
+456
+00:34:16,170 --> 00:34:20,250
+بتكون لو البكتيريا جيليكوز fermentation لكن non
+
+457
+00:34:20,250 --> 00:34:23,990
+-lactose fermenter بتكون red على yellow لكن لو
+
+458
+00:34:23,990 --> 00:34:28,050
+كانت جيليكوز و lactose fermentation بتكون yellow
+
+459
+00:34:28,050 --> 00:34:33,330
+على yellow ا على ا هذا بعد 24 ساعة لكن خلال أول 12
+
+460
+00:34:33,330 --> 00:34:42,970
+ساعة لو كانت lactoseأو non-lactose fermenter ستكون
+
+461
+00:34:42,970 --> 00:34:48,130
+A على A عشان هي كم نقرأ النتيجة عند 24 ساعة هدنا
+
+462
+00:34:48,130 --> 00:34:53,470
+هنتكلم في هذا الموضوع بالنسبة لو البكتيريا كانت
+
+463
+00:34:53,470 --> 00:34:57,740
+بالاصل non-gelicose fermenter هذا اتكلمنا عنههذا
+
+464
+00:34:57,740 --> 00:35:00,940
+كله لو كانت البكتيريا في الأصل هي Gelicose
+
+465
+00:35:00,940 --> 00:35:04,540
+Fermenter لكن لو البكتيريا هي ماعندها القدرة انها
+
+466
+00:35:04,540 --> 00:35:08,320
+تعمل fermentation لأبسط سكر الجليكوز يبقى أكيد مش
+
+467
+00:35:08,320 --> 00:35:12,840
+هتعمل fermentation للسكر الثنائي اللكتاز والسكراز
+
+468
+00:35:12,840 --> 00:35:17,680
+فبالتالي هتروح لمين هتروح للبيبتون هتعمله
+
+469
+00:35:17,680 --> 00:35:21,900
+deamination هيتكون عندي alkaline product هيتحول
+
+470
+00:35:21,900 --> 00:35:25,910
+لون ال media للون redهيصير اللون الـ Red قلنا الـ
+
+471
+00:35:25,910 --> 00:35:30,050
+Deamination للبيبتون هيصير في الـ Slant في الـ
+
+472
+00:35:30,050 --> 00:35:33,870
+Aerobic condition يبقى هيتحول اللون الـ Slant للون
+
+473
+00:35:33,870 --> 00:35:37,850
+الـ Red لكن البط مش هيصله أي تغير No, it changed
+
+474
+00:35:37,850 --> 00:35:41,770
+بقوللي ممكن برضه لو البكتيريا في الأصل non
+
+475
+00:35:41,770 --> 00:35:45,430
+-gelicose fermenter ممكن إنها لو ما عندها القدرة
+
+476
+00:35:45,430 --> 00:35:48,110
+إنها تعمل للـ Gelicose فبتعرفش هتعمل للاكتوز ولا
+
+477
+00:35:48,110 --> 00:35:53,880
+للسكراز أكيد هتعمل Deamination للبيبتون فيالـ
+
+478
+00:35:53,880 --> 00:35:58,380
+aerobic condition في الظروف الهوائية هيتحول لون
+
+479
+00:35:58,380 --> 00:36:02,260
+الـ slant للون red طيب لو البكتيريا ما اكتفت
+
+480
+00:36:02,260 --> 00:36:07,000
+بحاجتها من المواد الغذائية بقوللي هتعمل
+
+481
+00:36:07,000 --> 00:36:11,340
+deamination للبيبتون في ظروف unaerobic condition
+
+482
+00:36:11,340 --> 00:36:17,360
+بشكل اضطراري يبقى هان صار عند deamination للبيبتون
+
+483
+00:36:17,360 --> 00:36:22,560
+في ظروف unaerobic condition لكنبشكل اضطراري يبقى
+
+484
+00:36:22,560 --> 00:36:25,440
+لو كانت البكتيريا non-gelicose fermenter ممكن تكون
+
+485
+00:36:25,440 --> 00:36:32,400
+red على not change نكتب K على not change أو red
+
+486
+00:36:32,400 --> 00:36:39,860
+على red اللي هي K على K يبقى هان النتيجة نكتبها K
+
+487
+00:36:49,430 --> 00:36:54,710
+هان K على not change لأنه احنا قلنا ال red نرمز له
+
+488
+00:36:54,710 --> 00:37:14,710
+بالرمز K alkaline اتكون و هان K على K طيب
+
+489
+00:37:15,350 --> 00:37:18,250
+هذا بالنسبة لو كانت البكتيريا non-glycose
+
+490
+00:37:18,250 --> 00:37:22,490
+fermenter إما تكون K على K أو ممكن تكون K على نهار
+
+491
+00:37:22,490 --> 00:37:28,610
+تتشانج وفسرنا كل نتيجة إيش بتكون بالظبط طيب نوادج
+
+492
+00:37:28,610 --> 00:37:31,970
+عملية ال fermentation قلنا إما أسد واتكلمنا إنه
+
+493
+00:37:31,970 --> 00:37:38,310
+بتكون لون يلو فيه لتيوب أو جاز طيب بالنسبة للجاز
+
+494
+00:37:38,310 --> 00:37:43,350
+كيف بدي أكشف عنه قلنا إن الجاز ممكن كنا نكشف عنه
+
+495
+00:37:43,350 --> 00:37:47,690
+عن طريق الدرهام التيوبلكن الدرهام تيوب فقط بقدر
+
+496
+00:37:47,690 --> 00:37:52,210
+احطها لو كانت ال media broth هاي slant slant أجار
+
+497
+00:37:52,210 --> 00:37:55,790
+it's you بيبقى في عندي نسبة أجار ما بقدر احط درهام
+
+498
+00:37:55,790 --> 00:37:59,850
+it's you كيف بدي أكشف على النواتج عملية ال
+
+499
+00:37:59,850 --> 00:38:03,730
+fermentation لو كان في عندي تكون gas بقوللي بتكون
+
+500
+00:38:03,730 --> 00:38:07,890
+cracks بتكون زي تشققات في ال media او ممكن ال
+
+501
+00:38:07,890 --> 00:38:12,210
+media تطلع لفوق هيك بعرف ان والله اتكون عندي gas
+
+502
+00:38:12,210 --> 00:38:19,150
+طيببالنسبة للـ H2S production الـ H2S production
+
+503
+00:38:19,150 --> 00:38:23,650
+احنا قلنا انه الهاي الميديا بتكشف على ال
+
+504
+00:38:23,650 --> 00:38:27,090
+fermentation بتكشف على ال deamination للبيبتون
+
+505
+00:38:27,090 --> 00:38:30,410
+قلنا برضه ال fermentation ممكن يكون acid و gas
+
+506
+00:38:30,410 --> 00:38:33,950
+وعرفنا كيف كل نتيجة أشبهون لونها أو كيف بيكون
+
+507
+00:38:33,950 --> 00:38:39,450
+الشكل لكن بالنسبة لـالـ H2S production طيب لو تم
+
+508
+00:38:39,450 --> 00:38:43,390
+إنتاج اللون الأسود احنا قلنا الـ H2S production
+
+509
+00:38:43,390 --> 00:38:48,830
+بتكون عندي black precipitate هل هقدر أشوف طبعا الـ
+
+510
+00:38:48,830 --> 00:38:57,850
+H2S production بيصير زي ما قلنا قبل الـ
+
+511
+00:38:57,850 --> 00:39:01,230
+H2S production قلنا فيه بيصير في ظروف أن European
+
+512
+00:39:01,230 --> 00:39:06,620
+condition بيكون في البطلو صار في عندي إنتاج اللون
+
+513
+00:39:06,620 --> 00:39:11,480
+الأسود هل هقدر أشوف اللون الأصفر الناتج من عملية
+
+514
+00:39:11,480 --> 00:39:14,400
+ال fermentation خاصة أن ال fermentation و ال H2S
+
+515
+00:39:14,400 --> 00:39:17,420
+production بيصيروا في الـ Un-aerobic condition
+
+516
+00:39:17,420 --> 00:39:22,300
+بيصيروا في البط لو صار في عندي تكون للون الأسود
+
+517
+00:39:22,300 --> 00:39:27,280
+الناتج من أنه اتكون ال H2S gas هل هقدر أشوف اللون
+
+518
+00:39:27,280 --> 00:39:33,300
+الأصفر في البط ولا لأ؟ بقولليلو عملت البكتيريا H2S
+
+519
+00:39:33,300 --> 00:39:39,120
+production بدي أكون عارفة إنه عملت Gelicose
+
+520
+00:39:39,120 --> 00:39:43,280
+fermentation يبقى طالما البكتيريا H2S production
+
+521
+00:39:43,280 --> 00:39:46,760
+positive يبقى هي Gelicose fermentation positive
+
+522
+00:39:46,760 --> 00:39:53,020
+لأن إنتاج الـH2S production يحصل في الوسط الحمضي
+
+523
+00:39:53,280 --> 00:39:58,480
+الناتج من عملية تخمير اللي هو السكريات فبالتالي و
+
+524
+00:39:58,480 --> 00:40:01,900
+اتكون acid صار في عندي acid ال condition هيتكون ال
+
+525
+00:40:01,900 --> 00:40:07,200
+H2S production يبقى بدنا نكون عارفين إذا اتكون عند
+
+526
+00:40:07,200 --> 00:40:11,160
+ال H2S لون اتكون لون أسود في البط على طول بكتوب
+
+527
+00:40:11,160 --> 00:40:16,240
+جنبها ان صار في عندي جليكوز fermentation لأن عملية
+
+528
+00:40:16,240 --> 00:40:21,260
+ال H2S production بتصير في الوسط الحمضي هذا
+
+529
+00:40:21,260 --> 00:40:22,180
+بالنسبة ل
+
+530
+00:40:26,150 --> 00:40:30,810
+الـ H2S production يبقى عريفة انها كل عملية عشان
+
+531
+00:40:30,810 --> 00:40:33,610
+نتيجة دي ammunition للبيبتون قولنا بيكون في عندي
+
+532
+00:40:33,610 --> 00:40:37,830
+اتكوين لون Red في ال slant ممكن يصير بشكل اضطراري
+
+533
+00:40:37,830 --> 00:40:42,690
+في البط ال fermentation لو صار في عندي لجليكوز فقط
+
+534
+00:40:42,690 --> 00:40:46,830
+جليكوز fermentation بدون ما يصير للاكتوز والسكراز
+
+535
+00:40:46,830 --> 00:40:51,890
+بتكون عندي في البط لون أصفر لكن في ال slant بيكون
+
+536
+00:40:51,890 --> 00:40:56,000
+في عنديلون أحمر نتيجة الـdeamination للـpeptide
+
+537
+00:40:56,000 --> 00:40:58,760
+لكن لو البكتيريا عندها القدرة إنها تعمل
+
+538
+00:40:58,760 --> 00:41:01,180
+fermentation لكل السكريات الموجودة في الـmedia
+
+539
+00:41:01,180 --> 00:41:05,180
+هلاقي كل tube لونه أصفر الـH2O acid production
+
+540
+00:41:05,180 --> 00:41:10,660
+قولنا لو اتكون راسب أسود في البط يبقى هذا معناه
+
+541
+00:41:10,660 --> 00:41:13,320
+إنه بدي أعرف إنه والله صار في عندي جليكوز
+
+542
+00:41:13,320 --> 00:41:17,500
+fermentation already لأن الـH2O acid production
+
+543
+00:41:17,500 --> 00:41:19,280
+بيصير في الـacidic condition
+
+544
+00:41:25,030 --> 00:41:32,450
+طيب فيها معلومة مهمة ان انا بقرأ النتائج لل triple
+
+545
+00:41:32,450 --> 00:41:38,910
+sugar iron agar عند 24 ساعة ممنوع اقرأ بعد 24 ساعة
+
+546
+00:41:38,910 --> 00:41:45,270
+و ممنوع اقرأ قبل 24 ساعة طيب لو قرأت بعد 24 ساعة
+
+547
+00:41:45,270 --> 00:41:49,110
+بقولي كل الكربوهيدرات الموجودة في ال media هتكون
+
+548
+00:41:49,110 --> 00:41:55,810
+تم استهلاكها will be depleted ال bacteria لووبرضه
+
+549
+00:41:55,810 --> 00:42:00,210
+البكتيريا هتلجأ بما انه خلصت بعد 24 ساعة هيكون خلص
+
+550
+00:42:00,210 --> 00:42:03,910
+الجليكوز already هتكون خلص اللاكتوز والسكراز
+
+551
+00:42:03,910 --> 00:42:08,030
+البكتيريا هتلجأ لمصادر تانية اللي هو ال بيبتون
+
+552
+00:42:08,030 --> 00:42:14,010
+وهتعمله دي ammunition فبالتالي لو كانت النتيجة A
+
+553
+00:42:14,010 --> 00:42:17,850
+على A لو كانت البكتيريا في الأصل جليكوز fermenter
+
+554
+00:42:17,850 --> 00:42:21,130
+عندها القدرة على تخمير اللاكتوز والسكراز قلنا
+
+555
+00:42:21,130 --> 00:42:25,220
+بتكون النتيجة A على Aبتعمل fermentation للجليكوز
+
+556
+00:42:25,220 --> 00:42:30,120
+خلال 12 ساعة خلال أول 12 ساعة بعد هيخلص الجليكوز
+
+557
+00:42:30,120 --> 00:42:33,160
+لأنه موجود في كمية قليلة هتروح للكتاز والسكراز
+
+558
+00:42:33,160 --> 00:42:37,320
+هينتج كمية أسد كبيرة هينتشر في كل tube اللون
+
+559
+00:42:37,320 --> 00:42:41,140
+الأصفر وهيتكون yellow في ال slant و yellow في البط
+
+560
+00:42:41,140 --> 00:42:45,680
+يبقى هتكون النتيجة a على a لو كانت النتيجة a على a
+
+561
+00:42:45,680 --> 00:42:50,950
+تمام هي بالاصل lactose fermenterورحت أنا قرأت
+
+562
+00:42:50,950 --> 00:42:55,570
+النتيجة بعد 24 ساعة بقول لي انه اكيد بعد 24 ساعة
+
+563
+00:42:55,570 --> 00:43:00,510
+هيخلص اللكتاز والسكراز هتروح البكتيريا لمصدر تاني
+
+564
+00:43:00,510 --> 00:43:03,750
+اللي هو ال بيبتون هتعمله deamination ال
+
+565
+00:43:03,750 --> 00:43:06,770
+deamination للبيبتون قلنا بيصير في ظروف europic
+
+566
+00:43:06,770 --> 00:43:11,830
+condition بيصير في الإسلاند بيتحول لون الإسلاند
+
+567
+00:43:11,830 --> 00:43:17,210
+للون red يبقى هتتحول النتيجة ال a على a ل k على a
+
+568
+00:43:17,210 --> 00:43:22,290
+فهفكرها أناإنه هي non-lactose fermenter لكن هي في
+
+569
+00:43:22,290 --> 00:43:25,510
+الأصل lactose fermenter لكن لأني أنا قرأت النتيجة
+
+570
+00:43:25,510 --> 00:43:32,790
+بعد 24 ساعة طيب لو قرأت قبل 24 ساعة بقولي لو قرأتي
+
+571
+00:43:32,790 --> 00:43:36,130
+على سبيل المثال كانت البكتيريا هي non-lactose or
+
+572
+00:43:36,130 --> 00:43:40,310
+saccharose fermenter كانت K على A وروحت أنا قرأتها
+
+573
+00:43:40,670 --> 00:43:45,010
+قبل 24 ساعة قبل 24 ساعة قلنا سواء كانت lactose
+
+574
+00:43:45,010 --> 00:43:47,970
+fermenter أو non-lactose fermenter بتكون A على A
+
+575
+00:43:47,970 --> 00:43:51,790
+لأنه فقط بيتم استهلاك الجليكوز خلال أول 12 ساعة
+
+576
+00:43:51,790 --> 00:43:57,250
+بيتحول بيتكون acid هذا النواتج عملية ال
+
+577
+00:43:57,250 --> 00:44:00,310
+fermentation acid بيصير في الان European condition
+
+578
+00:44:00,310 --> 00:44:06,190
+لكن اللون الأصفر بيمتد في كل tube فبالتالي هفكر
+
+579
+00:44:06,190 --> 00:44:12,110
+النتيجة A على Aيبقى لو أنا قرأت قبل 24 ساعة وكانت
+
+580
+00:44:12,110 --> 00:44:15,390
+البكتيريا بالاصل هي non-lactose fermenter يبقى
+
+581
+00:44:15,390 --> 00:44:19,630
+النتيجة المفترض أنا أقرأها K على A لو قرأتها قبل
+
+582
+00:44:19,630 --> 00:44:23,610
+24 ساعة هتكون النتيجة A على A فبالتالي هيصير في
+
+583
+00:44:23,610 --> 00:44:27,050
+عندي false result عشان هيك لازم أقرأ النتيجة عند
+
+584
+00:44:27,050 --> 00:44:33,070
+24 ساعة مش قبل 24 ساعة و مش بعد 24 ساعة قلنا بعد
+
+585
+00:44:33,070 --> 00:44:37,490
+24 ساعة لو كانت البكتيريا A على A هتبين عندي كأنها
+
+586
+00:44:37,490 --> 00:44:42,800
+K على Aلكن لو قرأت قبل 24 ساعة و كانت في الأصل هي
+
+587
+00:44:42,800 --> 00:44:46,760
+K على A هتبين عندي كأنها A على A فبالتالي كل
+
+588
+00:44:46,760 --> 00:44:48,880
+النتائج هتكون عندي غلط
+
+589
+00:44:54,580 --> 00:44:58,900
+نرجع على السلايدات kliger iron agar اللي kliger هو
+
+590
+00:44:58,900 --> 00:45:02,360
+اسم العالم iron لأنه بدي أكشف فيها على الـ H2O
+
+591
+00:45:02,360 --> 00:45:06,880
+production agar لأنه أكيد في الميديا هي فيها نسبة
+
+592
+00:45:06,880 --> 00:45:11,360
+agar بنسبة واحد ونص في المية ال triple sugar iron
+
+593
+00:45:11,360 --> 00:45:15,280
+agar لأنه ال triple فيها تلت سكريات قولنا ال
+
+594
+00:45:15,280 --> 00:45:20,080
+dextrose اللي هو الجليكوز أو اللكتوز والسكروزIron
+
+595
+00:45:20,080 --> 00:45:23,460
+بدي أكشف على الـ H2S production و الأجار لأن فيها
+
+596
+00:45:23,460 --> 00:45:27,600
+نسبة أجار واحد و نصف في الميديا واحد و نصف بالمية
+
+597
+00:45:27,600 --> 00:45:31,780
+Cligar iron agar and triple sugar iron agar are
+
+598
+00:45:31,780 --> 00:45:36,140
+widely used in the identification of gram negative
+
+599
+00:45:36,140 --> 00:45:40,500
+bacteria particularly the enterobacteriasis هذه
+
+600
+00:45:40,500 --> 00:45:45,420
+الميديا بتستخدم للتعريف للgram negative bacteria
+
+601
+00:45:45,420 --> 00:45:48,230
+خاصة ال enterobacteriasisقلنا ان الـ
+
+602
+00:45:48,230 --> 00:45:51,570
+Enterobacteriasis هي الجرام نيجاتيف بسلاي تعتبر
+
+603
+00:45:51,570 --> 00:45:56,670
+enteric بكتيريا بكشف من خلال أو بعمل تعريف للـ
+
+604
+00:45:56,670 --> 00:45:59,590
+Enterobacteriasis من خلال triple sugar iron agar
+
+605
+00:45:59,590 --> 00:46:03,870
+أو كليجر ايرون اجار هذه ال media the media are
+
+606
+00:46:03,870 --> 00:46:08,150
+identical الميديتين بتشابه بعض نفس الاشي بيتشابه
+
+607
+00:46:08,150 --> 00:46:11,710
+لكن الاختلاف ان ال triple sugar iron agar بتحتوي
+
+608
+00:46:11,710 --> 00:46:15,970
+على استكاروزهو الإختلاف بالإضافة للـ Dextrose والـ
+
+609
+00:46:15,970 --> 00:46:20,930
+Lactose الموجود في الجليجر ايرون اجار كيف انا
+
+610
+00:46:20,930 --> 00:46:25,170
+بصبها ال media قلنا ال media بصبها slant تمام كيف
+
+611
+00:46:25,170 --> 00:46:29,630
+بزرعها بعمل stabbing لل بط للبط بعمل stabbing
+
+612
+00:46:29,630 --> 00:46:33,310
+بعدين بعمل بطلة على ال slant surface و بعمل زيجا
+
+613
+00:46:33,310 --> 00:46:37,530
+زاج هك احنا قلنا ان نزرع ال slant tube هك
+
+614
+00:46:37,530 --> 00:46:41,470
+البكتيريا بتتعرض ل anaerobicEnvironment تقولنا
+
+615
+00:46:41,470 --> 00:46:45,890
+بتتعرض لظروف لا هوائية من خلال البط وظروف هي
+
+616
+00:46:45,890 --> 00:46:50,090
+هوائية اللي هي الـAerobic من خلال الاسلاند الفينار
+
+617
+00:46:50,090 --> 00:46:53,870
+Red موجود ك Indicator ك BH Indicator في ال media
+
+618
+00:46:53,870 --> 00:46:57,210
+وقلنا في عندي كمان H2S Indicator اللي هو الferric
+
+619
+00:46:57,210 --> 00:47:01,030
+ammonium citrate لو البكتيريا في الأصل هي non
+
+620
+00:47:01,030 --> 00:47:04,310
+-fermenter ما بتعمل fermentation للجليكوز ولا
+
+621
+00:47:04,310 --> 00:47:10,090
+للكتاز ولا للسكراز أكيد هتلجأ لمصدر البيبتونزي الـ
+
+622
+00:47:10,090 --> 00:47:13,170
+Sodomonas احنا قلنا Sodomonas تعتبر non-gelicose
+
+623
+00:47:13,170 --> 00:47:18,230
+fermenter فبالتالي هتنجأ للبيبتون هتنمو فقط على ال
+
+624
+00:47:18,230 --> 00:47:22,230
+slant تمام لأنه احنا قلنا انه هي Sodomonas
+
+625
+00:47:22,230 --> 00:47:25,830
+obligate aerobic bacteria فبالتالي هتنمو على ال
+
+626
+00:47:25,830 --> 00:47:29,970
+slant في ال aerobic condition هتعمل degradation
+
+627
+00:47:29,970 --> 00:47:34,450
+للبروتين للبيبتون قلنا هتعمل deamination للبيبتون
+
+628
+00:47:34,450 --> 00:47:39,360
+هيتكون alkaline product هيتحولاللون ال media للون
+
+629
+00:47:39,360 --> 00:47:46,080
+أحمر في ال slant فقط فبالتالي هيك بتكون ال اللي هي
+
+630
+00:47:46,080 --> 00:47:50,080
+نتيجة ال Sodomonas انه صار في عندي deamination
+
+631
+00:47:50,080 --> 00:47:56,440
+للبيبتون هيتحول لون ال slant للون أحمر مكونات ال
+
+632
+00:47:56,440 --> 00:47:59,480
+media مكونات ال media في عندي enzymatic digest of
+
+633
+00:47:59,480 --> 00:48:02,760
+غازين enzymatic digest of animal tissue هدول
+
+634
+00:48:02,760 --> 00:48:06,990
+يعتبروامصادر للتغذية مصدر لل كربون وال نيتروجن
+
+635
+00:48:06,990 --> 00:48:10,290
+source اللازم لنمو البكتيريا هاي لون ال media
+
+636
+00:48:10,290 --> 00:48:18,030
+لونها جاي على أورنج تمام يست انرشت بيبتون قولنا
+
+637
+00:48:18,030 --> 00:48:23,770
+البيبتون يعتبر كمصدر للهو ال nitrogen source وكمان
+
+638
+00:48:23,770 --> 00:48:27,580
+لل carbon sourceوقلنا عشان البكتيريا تعمله بدي
+
+639
+00:48:27,580 --> 00:48:30,260
+أكشف على الـ deamination للبيبطان فبالتالي أكيد
+
+640
+00:48:30,260 --> 00:48:34,000
+لازم يكون في عيندي بيبطان في الميديا ديكستراز
+
+641
+00:48:34,000 --> 00:48:38,300
+واللاكتاز موجودين في ال triple وكلجر ايرون اجار
+
+642
+00:48:38,300 --> 00:48:41,640
+قلنا الديكستراز موجود في كمية قليلة زي ما ملاحظين
+
+643
+00:48:41,640 --> 00:48:47,340
+1 من 10% اللاكتاز والسكراز موجود بكمية أعلى 1%
+
+644
+00:48:47,340 --> 00:48:50,520
+السكراز مش موجود في الكلجر ايرون اجار قلنا
+
+645
+00:48:50,520 --> 00:48:54,930
+الإختلاف بين الكلجر و ال tripleإنه استكالوز مش
+
+646
+00:48:54,930 --> 00:48:58,650
+موجود في الجليجر لكن موجود في ال triple sugar iron
+
+647
+00:48:58,650 --> 00:49:03,430
+agar في اندفيرك أمونيوم سايتريات اللي هو ال H2S
+
+648
+00:49:03,430 --> 00:49:07,510
+indicator صوديوم كلورايد عشان يحافظلي على ال
+
+649
+00:49:07,510 --> 00:49:10,470
+osmotic pressure صوديوم ثيو سلفات ال substrate
+
+650
+00:49:10,470 --> 00:49:13,950
+اللي هيشتغل عليها أنزيم الصوديوم ثيو سلفات
+
+651
+00:49:13,950 --> 00:49:18,090
+productive عشان أكشف على ال H2S production في نار
+
+652
+00:49:18,090 --> 00:49:21,990
+red قلنا اللي هو ال BH indicator قلنا بيكونفي ال
+
+653
+00:49:21,990 --> 00:49:28,150
+media لونه بيكون ال في ال طبعا في ال neutral لونه
+
+654
+00:49:28,150 --> 00:49:32,470
+orange وهو لون ال pH للميديا بدون ما أنا أزرعها
+
+655
+00:49:32,470 --> 00:49:35,990
+بتكون سبعة وأربع فبالتالي هنا سبعة وأربع بيكون لون
+
+656
+00:49:35,990 --> 00:49:42,130
+orange يبقى لون ال media هو لون orange لون برتقالي
+
+657
+00:49:42,130 --> 00:49:46,390
+الأجار أكيد بما أنه هي إسلانت يبقى نسبة الأجار
+
+658
+00:49:46,390 --> 00:49:52,230
+واحد ونصف في الميةهان ذكرلي كل مكوّن من المكونات
+
+659
+00:49:52,230 --> 00:49:55,470
+إيش؟ فإنتوا قولنا ال casein ال animal tissue ال
+
+660
+00:49:55,470 --> 00:49:59,530
+yeast and the restعشان اوفر اللي هو الـ nitrogen و
+
+661
+00:49:59,530 --> 00:50:02,010
+الـ carbon و الـ vitamin و الـ source اللي لازم
+
+662
+00:50:02,010 --> 00:50:05,330
+لنمو البكتيريا قولنا اللي triple sugar iron agar
+
+663
+00:50:05,330 --> 00:50:08,330
+بتحتوي على تلت أنواع من السكريات ديكستروز، لكتاز،
+
+664
+00:50:08,330 --> 00:50:11,710
+سكراز، لكن الـkliger iron agar فقط ديكستروز و
+
+665
+00:50:11,710 --> 00:50:14,770
+لكتاز لو البكتيريا عندها القدرة انها تعمل
+
+666
+00:50:14,770 --> 00:50:19,050
+fermentation لهاي السكريات هيتكون acid بقدر اكشف
+
+667
+00:50:19,050 --> 00:50:22,030
+عنه من خلال وجود الـ phenol red اللي هو الـ pH
+
+668
+00:50:22,030 --> 00:50:25,130
+indicatorالسوديوم سيوسالفات هو الـ substrate اللى
+
+669
+00:50:25,130 --> 00:50:29,550
+هيشتغل عليه الانزيم هيعمل لو موجود فيه البكتيريا
+
+670
+00:50:29,550 --> 00:50:33,630
+هيعمله اختيزة ل hydrogen sulfide ال hydrogen
+
+671
+00:50:33,630 --> 00:50:38,430
+sulfide هيتفاعل مع ال iron salt فبالتالي هيتكون
+
+672
+00:50:38,430 --> 00:50:42,600
+عندي black precipitate زى ما ذكرناه فيوشرحناها في
+
+673
+00:50:42,600 --> 00:50:46,720
+ميديا SIM Ferric Ammonium Citrate هو الـ H2S
+
+674
+00:50:46,720 --> 00:50:50,080
+Indicator اللي هيرتبط مع الـ H2S Gas وهيعطينا
+
+675
+00:50:50,080 --> 00:50:53,580
+black precipitate Sodium Chloride أي ميديا فيها
+
+676
+00:50:53,580 --> 00:50:56,520
+Sodium Chloride عشان يحافظ على الـ Osmotic
+
+677
+00:50:56,520 --> 00:51:03,840
+Pressure الأجار يعتبر ك solidifying agentهذه
+
+678
+00:51:03,840 --> 00:51:09,180
+الميديا بتعملي identification لـ Antirobacteriasi
+
+679
+00:51:09,180 --> 00:51:13,360
+من خلال هذه الميديا بكشف على قدرة البكتيريا انها
+
+680
+00:51:13,360 --> 00:51:19,300
+اتكون او انه اتكون gas نتيجة fermentation للشجر
+
+681
+00:51:19,300 --> 00:51:24,380
+انها اتعمل fermentation لللاكتوز او السكاروز كمان
+
+682
+00:51:24,380 --> 00:51:28,140
+انها اتكون اللي هو ال hydrogen sulfide الـH2S gas
+
+683
+00:51:33,190 --> 00:51:37,110
+principle for fermentative organism jelly cause is
+
+684
+00:51:37,110 --> 00:51:41,670
+the first sugar catabolized بقوللي في هاي ال media
+
+685
+00:51:41,670 --> 00:51:45,210
+انا بكشف على ال fermentation احنا قولنا ان في هاي
+
+686
+00:51:45,210 --> 00:51:48,950
+ال media انا بكشف على ال fermentation هلأ
+
+687
+00:51:48,950 --> 00:51:54,310
+البكتيريا هتستهلك اول ما هتروح هتروح لأبسط الطرق
+
+688
+00:51:54,310 --> 00:51:59,570
+حتى تحصل على الطاقة والغذاء فأكيد هتبدأ بأبسط مركب
+
+689
+00:51:59,570 --> 00:52:02,730
+اللي هو الجليكواز هتبدأ بال Dextrose اللي احنا
+
+690
+00:52:02,730 --> 00:52:07,890
+قلناه يعتبر simple sugar هتعمله fermentation لو
+
+691
+00:52:07,890 --> 00:52:10,690
+البكتيريا عندها القدرة انها تعمل fermentation لهذا
+
+692
+00:52:10,690 --> 00:52:14,990
+الجليكواز هتعمل fermentationهيتكون عندى acid
+
+693
+00:52:14,990 --> 00:52:20,470
+فبالتالي هيتحول لون ال media للون أصفر if glycose
+
+694
+00:52:20,470 --> 00:52:24,130
+is used up اذا تم استهلاك الجليكوز a bacterium
+
+695
+00:52:24,130 --> 00:52:27,230
+reach a metabolic fork in the rod البكتيريا هتوصل
+
+696
+00:52:27,230 --> 00:52:33,770
+للحظة ان هي هتكون زى الشوكة في الطريقتمام هذا
+
+697
+00:52:33,770 --> 00:52:37,130
+بالنسبة لأول إشي ان احنا قلنا ان البكتيريا هتروح
+
+698
+00:52:37,130 --> 00:52:43,430
+لأول مصدر اللي هو الجليكوز هتستهلك الجليكوز هيتكون
+
+699
+00:52:43,430 --> 00:52:47,870
+أسد هيتحول لون ال media فيه البط وهينتشر كمان في
+
+700
+00:52:47,870 --> 00:52:51,990
+ال إسلانت للون يلو if an organism produce the
+
+701
+00:52:51,990 --> 00:52:56,550
+enzyme to hydrolyze this saccharide lactose the
+
+702
+00:52:56,550 --> 00:53:01,050
+organism will continue fermenting to assist in ATP
+
+703
+00:53:01,050 --> 00:53:06,320
+generationبقول لي لو البكتيريا لو خلص طبعا اكيد ال
+
+704
+00:53:06,320 --> 00:53:10,580
+ال جيليوكوز موجود بكمية قليلة لو خلص ال dextrose
+
+705
+00:53:10,580 --> 00:53:14,980
+هتروح تستهلك اللكتوز او السكاروز او الاتنين معاها
+
+706
+00:53:14,980 --> 00:53:19,120
+حسب الانزيمات الموجودة عند البكتيريا تمام هذا
+
+707
+00:53:19,120 --> 00:53:26,140
+بالنسبة للكتوز و السكاروز however if the gelicose
+
+708
+00:53:26,140 --> 00:53:29,440
+is limiting and the organism doesn't produce them
+
+709
+00:53:29,720 --> 00:53:32,940
+necessary enzyme to catabolize lactose the
+
+710
+00:53:32,940 --> 00:53:36,780
+organism can utilize the protein in the media via
+
+711
+00:53:36,780 --> 00:53:41,680
+the process of deamination بقولي لو الجليكوز خلص
+
+712
+00:53:41,680 --> 00:53:44,740
+والبكتيريا ماعندها القدرة انها تكسر انزيمات
+
+713
+00:53:44,740 --> 00:53:47,280
+اللاكتوز يعني لو البكتيريا في الأصل الجليكوز
+
+714
+00:53:47,280 --> 00:53:49,360
+fermentation لكن ماعندها القدرة انها تعمل
+
+715
+00:53:49,360 --> 00:53:53,260
+fermentation لا اللاكتوز ولا السكراز أكيد هتستهلك
+
+716
+00:53:53,260 --> 00:53:57,340
+البروتين البيبتون الموجود في الميديا هتعملهدى
+
+717
+00:53:57,340 --> 00:54:01,480
+amination ويتحول لون ال slant للون ده يبقى هذا
+
+718
+00:54:01,480 --> 00:54:07,760
+بالنسبة لل principle اللى شرحناه سابقا بالنسبة
+
+719
+00:54:07,760 --> 00:54:10,920
+للخطوات قولنا بنعمل زراع على ال triple sugar iron
+
+720
+00:54:10,920 --> 00:54:14,120
+agaric slant من خلال ان انا بعمل stabbing لالبط و
+
+721
+00:54:14,120 --> 00:54:17,880
+ال slant بزرعه زيك زاك بعمل incubation لمدة 24
+
+722
+00:54:17,880 --> 00:54:23,330
+hours ممنوع قبل اطلع ممنوع بعدقلنا إن الـ timing
+
+723
+00:54:23,330 --> 00:54:27,690
+is critical في قراءة لـ triple sugar iron agar أو
+
+724
+00:54:27,690 --> 00:54:32,310
+لـ clagar iron agar لو أنا قرأت قبل 24 ساعة an
+
+725
+00:54:32,310 --> 00:54:36,650
+early reading could reveal yellow throughout the
+
+726
+00:54:36,650 --> 00:54:40,930
+media بقوللي إنه لو قرأت قبل 24 ساعة بيكون تم
+
+727
+00:54:40,930 --> 00:54:45,930
+استهلاك فقط الجليكوز صلوا fermentation هيتحول لون
+
+728
+00:54:45,930 --> 00:54:50,260
+الوسط للون yellowفبالتالي هذا هيصير في الـ Un
+
+729
+00:54:50,260 --> 00:54:53,240
+-aerobic condition و هيمتد اللون الأصفر في كل سيوب
+
+730
+00:54:53,240 --> 00:54:56,980
+فبالتالي كل الـ media هيكون لونها أصفر هيج أنا
+
+731
+00:54:56,980 --> 00:55:00,900
+leading one to conclude that the organism is a
+
+732
+00:55:00,900 --> 00:55:05,180
+lactose fermenter when it simply may not yet have
+
+733
+00:55:05,180 --> 00:55:09,820
+exercised the glycose بقوللي أنا هفكر أنه ال
+
+734
+00:55:09,820 --> 00:55:15,110
+organism صار عندهأو عمل fermentation للـ lactose
+
+735
+00:55:15,110 --> 00:55:20,550
+لكن هو فقط لحد الآن استهلك الجليكوز لو أنا قرأت
+
+736
+00:55:20,550 --> 00:55:25,610
+بعد 24 ساعة a reading after the لو أنا قرأت بعد 24
+
+737
+00:55:25,610 --> 00:55:28,910
+ساعة the lactose has been depleted بقولني أن ال
+
+738
+00:55:28,910 --> 00:55:34,220
+lactose بسله استهلاك كلهcould reveal a yellow pot
+
+739
+00:55:34,220 --> 00:55:37,620
+and red slant leading one to fossil conclude that
+
+740
+00:55:37,620 --> 00:55:40,740
+the organism is glucose only fermenter لو أنا قرأت
+
+741
+00:55:40,740 --> 00:55:45,240
+بعد 24 ساعات لكتاز والسكراز هيتم استهلاكهم
+
+742
+00:55:45,240 --> 00:55:48,120
+فبالتالي البكتيريا هتروح لمصادر تانية اللي هي ال
+
+743
+00:55:48,120 --> 00:55:51,560
+بيبتون فبالتالي هيصير في عندي deamination للبيبتون
+
+744
+00:55:51,560 --> 00:55:55,060
+في ال slant هيتحول لون ال slant للون red لكن في ال
+
+745
+00:55:55,060 --> 00:55:59,140
+pot هيكون yellow فبالتالي انا هفكر هذه النتيجة K
+
+746
+00:55:59,140 --> 00:56:04,450
+على A ان فقط البكتيرياعندها القدرة إنها تستعمل
+
+747
+00:56:04,450 --> 00:56:08,870
+fermentation للجليكوز وهي non-lactose fermenter
+
+748
+00:56:08,870 --> 00:56:14,330
+فهك هيصير في عندي falsely result بالنسبة للنتائج
+
+749
+00:56:14,330 --> 00:56:20,650
+لو اتكون فيه البطلون yellow في ال slant يلو يبقى
+
+750
+00:56:20,650 --> 00:56:24,430
+هك بعرف إن البكتيريا عملت gelicose و lactose
+
+751
+00:56:24,430 --> 00:56:29,360
+وsaccharose fermentationأتكون عندي acid هذا ال
+
+752
+00:56:29,360 --> 00:56:34,380
+acid اتحول لون ال وسط للون أصفر نتيجة وجود الفينال
+
+753
+00:56:34,380 --> 00:56:38,000
+red انتشر في ال slant وفي ال butt برمز لقيله
+
+754
+00:56:38,000 --> 00:56:43,060
+بالرمز A على A تمام هذا بالنسبة لأول رمز هذا لو
+
+755
+00:56:43,060 --> 00:56:48,640
+قرأت النتائج عند 24 ساعة لأنه لو أنا قرأت هان عند
+
+756
+00:56:48,640 --> 00:56:52,480
+أول 12 ساعة معناه أن فقط صار في عندي استهلاك
+
+757
+00:56:52,480 --> 00:56:57,340
+للجليكوز هذا كله بتتكلم عند 24 ساعةطيب لو لاحظت في
+
+758
+00:56:57,340 --> 00:57:00,580
+ال bot في عندي yellow لكن في ال slant في عندي red
+
+759
+00:57:00,580 --> 00:57:03,820
+في ال في ال yellow bot معناه انه صار في عندي فقط
+
+760
+00:57:03,820 --> 00:57:07,560
+gelatinous fermentation اتكون في عندي acid في ال
+
+761
+00:57:07,560 --> 00:57:10,820
+slant اتكون red يبقى صار في عندي deamination
+
+762
+00:57:10,820 --> 00:57:15,340
+لبيبتون بشكل aerobically بشكل في ظروف ال aerobic
+
+763
+00:57:15,340 --> 00:57:21,080
+condition تمام الرمز بما انه yellow بنحط A بما انه
+
+764
+00:57:21,080 --> 00:57:26,070
+hundred يبقى بنحط KRed على Red يبقى ما أصار في
+
+765
+00:57:26,070 --> 00:57:29,570
+عندي fermentation حتى ما أشوف لون Red في ال بط أو
+
+766
+00:57:29,570 --> 00:57:33,030
+في الإسلان يبقى ما أصار في عندي ماشوفة لون ال
+
+767
+00:57:33,030 --> 00:57:38,150
+yellow يبقى ما أصار في عندي fermentation إيش معناه
+
+768
+00:57:38,150 --> 00:57:40,430
+في ال Red في الإسلان معناه إنه صار في عندي
+
+769
+00:57:40,430 --> 00:57:44,310
+deamination للبيبتون في الظروف الهوائية في ال
+
+770
+00:57:44,310 --> 00:57:49,370
+European conditionالبكتيريا ماقتفت بحاجتها في
+
+771
+00:57:49,370 --> 00:57:52,810
+المواد الغذائية فعملت deamination للبيبتون بشكل
+
+772
+00:57:52,810 --> 00:57:56,890
+اضطراري في الظروف الـ anaerobic condition يبقى صار
+
+773
+00:57:56,890 --> 00:58:00,010
+في عندي بيبتون deamination aerobically and
+
+774
+00:58:00,010 --> 00:58:02,790
+anaerobically اتكون في عندي alkaline product
+
+775
+00:58:02,790 --> 00:58:07,410
+فاتحول اللونالميديا للون أحمر نتيجة تكون
+
+776
+00:58:07,410 --> 00:58:12,130
+الألقالييني برودك تبقى الرمز K على K هذه البكتيريا
+
+777
+00:58:12,130 --> 00:58:15,430
+بما أنه في الأصل هي non fermentation يبقى هي مش من
+
+778
+00:58:15,430 --> 00:58:19,290
+ال انتيرو بكترياسي لأن الانتيرو بكترياسي هي تعمل
+
+779
+00:58:19,290 --> 00:58:23,810
+جليكوز fermentation كلها لو ال red كان slant لكن
+
+780
+00:58:23,810 --> 00:58:27,370
+في البط ماصار في عندي أي تغير يبقى هان بحكي أنه
+
+781
+00:58:27,370 --> 00:58:30,530
+ماصار في عندي fermentation لكن صار في عنديهذه
+
+782
+00:58:30,530 --> 00:58:34,190
+اميناشن للبيبتون لكن فقط في الـ aerobic condition
+
+783
+00:58:34,190 --> 00:58:37,670
+تكون عندي alkaline product تتحول لون ال media أو
+
+784
+00:58:37,670 --> 00:58:41,670
+لون ال slant فقط للون أحمر بما أن مصر في عندي
+
+785
+00:58:41,670 --> 00:58:44,030
+fermentation يبقى هي مش من ال interior bacteria
+
+786
+00:58:44,030 --> 00:58:48,410
+سيقولنا برمز لها بالرمز K على no exchange لو ما
+
+787
+00:58:48,410 --> 00:58:53,510
+اتغير في عندي ال في ال slant أو في البطمعناه إما
+
+788
+00:58:53,510 --> 00:58:59,050
+أن البكتيريا بتنمو بشكل بطيء أو بالاصل أنا ما زرعت
+
+789
+00:58:59,050 --> 00:59:01,970
+بكتيريا هي بالاصل مش من عائلة الـ Enterobacterias
+
+790
+00:59:01,970 --> 00:59:06,990
+لأن أنا طبعا ال بكتيريا كل ال Enterobacterias
+
+791
+00:59:06,990 --> 00:59:10,950
+بتنمو عادي عند 24 ساعة بتكون نامية فبالتالي not
+
+792
+00:59:10,950 --> 00:59:13,750
+change على not change بحكي أنه not from
+
+793
+00:59:13,750 --> 00:59:17,250
+Enterobacterias قلنا لـ Black precipitates برمز
+
+794
+00:59:17,250 --> 00:59:23,030
+لإله بالرمز H2Sالـ Sulphur Reduction أو اللي هو
+
+795
+00:59:23,030 --> 00:59:25,430
+لما يصير في ندي الـ Reduction لالـ Sulphur قولنا
+
+796
+00:59:25,430 --> 00:59:28,310
+هذا بيصير في الـ Acidic Condition الناتج من ال
+
+797
+00:59:28,310 --> 00:59:31,670
+fermentation للجليكوز أو اللاكتوز أو السكاروز
+
+798
+00:59:31,670 --> 00:59:35,590
+فبالتالي لو تكون اللون الأسود لازم أكتب جنبها على
+
+799
+00:59:35,590 --> 00:59:41,170
+طول Aاللي هي في الـ Bot بحط جنبها A ممنوع أحط K
+
+800
+00:59:41,170 --> 00:59:45,410
+لأن الـ H2S بال reduction بيسوير في ظروف acidic
+
+801
+00:59:45,410 --> 00:59:50,110
+condition يبقى ممنوع أشوف نتيجة K على K مع الـ H2S
+
+802
+00:59:50,110 --> 00:59:53,750
+لأن الـ H2S بيسير في ظروف acidic condition يبقى من
+
+803
+00:59:53,750 --> 00:59:59,760
+حتى ما أشوف الـ H2S بحط في ال Bot Aلو اتكون في
+
+804
+00:59:59,760 --> 01:00:03,560
+عندي تشققات في ال media بحط بعرف انه والله ناتج
+
+805
+01:00:03,560 --> 01:00:08,120
+عملية ال fermentation acid مع gas فبضيف رمز اللي
+
+806
+01:00:08,120 --> 01:00:14,020
+هو ال gas جنب اللي هي الرمز A على A مثلا او K على
+
+807
+01:00:14,020 --> 01:00:18,180
+A بحط رمز جنب الرمز اللي هو ال gas معناه انه صار
+
+808
+01:00:18,180 --> 01:00:21,720
+في عندي من نواتج عملية ال fermentation كمان acid
+
+809
+01:00:22,270 --> 01:00:28,310
+هذا بالنسبة لكل الأشياء المحتمل انها تكون اول
+
+810
+01:00:28,310 --> 01:00:31,790
+رمزان هذا الرمز وهذا الرمز معناه انه هي من عائلة
+
+811
+01:00:31,790 --> 01:00:35,850
+الانتيرو بكترياسي لكن هذول التلاتة مش من عائلة
+
+812
+01:00:35,850 --> 01:00:38,510
+الانتيرو بكترياسي هذول التنين من عائلة الانتيرو
+
+813
+01:00:38,510 --> 01:00:42,270
+بكترياسي هي بتكون lactose fermenter هي بتكون non
+
+814
+01:00:42,270 --> 01:00:45,550
+lactose fermenter زي البروتيا السلمونيلا الشيجيلة
+
+815
+01:00:45,550 --> 01:00:49,710
+لكن هان بتكون من lactose fermenter الكوليفارم
+
+816
+01:00:50,090 --> 01:00:54,650
+التلاتة هدول مش من عائلة ال انتيرو بكترياسي طب
+
+817
+01:00:54,650 --> 01:01:01,690
+نيجي للنتائج أول واحدة بقولي ان هي رمزها K على A
+
+818
+01:01:01,690 --> 01:01:05,530
+تمام عندي لون اتكون لون Yellow و اتكون لون Red
+
+819
+01:01:05,530 --> 01:01:09,010
+يبقى هي K على A يبقى هي من عائلة ال انتيرو
+
+820
+01:01:09,010 --> 01:01:12,030
+بكترياسي اللي هي non-lactose fermenter زي
+
+821
+01:01:12,030 --> 01:01:16,050
+المارجانيلا مارجاني تمام ما اتكون في عندي جاز يبقى
+
+822
+01:01:16,050 --> 01:01:21,420
+خلاص K على A هاي البكتيريا Kعلى no change إذا
+
+823
+01:01:21,420 --> 01:01:25,780
+ملاحظين لون ال media تحت نفس هاي هاي ك control هاي
+
+824
+01:01:25,780 --> 01:01:28,760
+مش مزروعة ك control لون ال media هيك بيكون جاي على
+
+825
+01:01:28,760 --> 01:01:32,600
+أورنج فبنلاحظ إنه في ال slant اتكون لون red لكن في
+
+826
+01:01:32,600 --> 01:01:37,040
+البطك كان نفس اللون هذا ما تغير no change يبقى هي
+
+827
+01:01:37,040 --> 01:01:40,700
+صدمانة سقير جنازة يبقى هذا الرمز هي مش من عيلة ال
+
+828
+01:01:40,700 --> 01:01:43,640
+interior bacteriasia وفعلا الصدمانة سقير جنازة مش
+
+829
+01:01:43,640 --> 01:01:46,900
+من عيلة ال interior bacteriasia هاي مش مزروعة ك
+
+830
+01:01:46,900 --> 01:01:51,910
+controlهذا يمكن أن يتكون لون H2S production قلنا
+
+831
+01:01:51,910 --> 01:01:56,810
+من حد ما أشوف الـ H2S بحمل جانبه A لأن الـ H2S
+
+832
+01:01:56,810 --> 01:01:59,970
+production يحدث في ظروف acid condition يبقى أكيد
+
+833
+01:01:59,970 --> 01:02:05,210
+يكون هناك سارج ليكوز fermentation فوق اتكوين اللون
+
+834
+01:02:05,210 --> 01:02:10,010
+الريد يبقى بحكي K على A مع الـ H2S K على A يبقى هي
+
+835
+01:02:10,010 --> 01:02:14,130
+من عائلة الـ Enterobacteraceae من عائلة الـ non
+
+836
+01:02:14,130 --> 01:02:18,050
+-lactose fermenter لأنها K على AK على A بقى هي non
+
+837
+01:02:18,050 --> 01:02:20,770
+-lactose fermentation اتكون عندي H2S production
+
+838
+01:02:20,770 --> 01:02:27,150
+يبقى احتمال كبير انها تكون هي البروتياز بأنه اتكون
+
+839
+01:02:27,150 --> 01:02:32,070
+عندي ال H2S production يبقى هي البروتياز لكن في
+
+840
+01:02:32,070 --> 01:02:36,130
+عندي برضه كمان السلمونيلا بفرق بينهم بفحص اليورياز
+
+841
+01:02:36,920 --> 01:02:39,540
+Equally فيين دي صار فيين دي هان تشقيقات إذا
+
+842
+01:02:39,540 --> 01:02:44,500
+ملاحظين في ال media يبقى بحط رمز ال gas تمام و بما
+
+843
+01:02:44,500 --> 01:02:47,800
+إنه في ال slant و في ال path تتكون لون أصفر و أصفر
+
+844
+01:02:47,800 --> 01:02:52,460
+يبقى a على a with gas يبقى ها هي النتيجة بنقولنا
+
+845
+01:02:52,460 --> 01:02:56,280
+ال gas و ال H2S بضيفها جنب الرمز K على A أو على A
+
+846
+01:02:56,280 --> 01:03:03,900
+على M هذا بالنسبة للنتائج نيجي لهدول هان الرمز
+
+847
+01:03:03,900 --> 01:03:09,340
+اللي هو Kعلى A بما أنه عندي أتكون H2S يبقى اللي
+
+848
+01:03:09,340 --> 01:03:15,720
+تحتها بتكون A يبقى K على A with H2S هان K على A
+
+849
+01:03:15,720 --> 01:03:25,800
+with H2S with gas كمان مع gas نكتب K
+
+850
+01:03:25,800 --> 01:03:30,650
+بما أنه لونه أحمر فوق على Aطبعا لأنه في اندي H2S
+
+851
+01:03:30,650 --> 01:03:33,510
+production فعلى طول بعرف أنه اللي تحت اللون
+
+852
+01:03:33,510 --> 01:03:41,950
+المفترض يكون أصفر اللي هو A fermentation مع
+
+853
+01:03:41,950 --> 01:03:48,830
+H2S
+
+854
+01:03:48,830 --> 01:03:51,490
+هذا بالنسبة لأول واحدة
+
+855
+01:03:58,590 --> 01:04:03,990
+النتيجة التانية هي نفسها لكن مع اتكون في عندي gas
+
+856
+01:04:15,630 --> 01:04:19,870
+يبقى بما أنه في عندي اللون الأسود يبقى H2S يتكون
+
+857
+01:04:19,870 --> 01:04:23,890
+في عندي فيه تشققات يبقى في عندي gas طبعا بالنسبة
+
+858
+01:04:23,890 --> 01:04:28,090
+للبط بما أنه أسود اللون H2S يبقى على طول بدي أعرف
+
+859
+01:04:28,090 --> 01:04:31,170
+ايه ممنوع أحط K في ال triple sugar iron agar ممنوع
+
+860
+01:04:31,170 --> 01:04:35,070
+يكون في عندي هن H2S و أحط K فقط ايه لأن ال
+
+861
+01:04:35,070 --> 01:04:38,450
+production H2S بيحصل في ظروف acid condition فوق
+
+862
+01:04:38,450 --> 01:04:45,070
+تكون اللون الأحمر يبقى K هن K اللون الأحمر فوق على
+
+863
+01:04:49,250 --> 01:04:57,250
+هنا تلاحظ ان كتكون
+
+864
+01:04:57,250 --> 01:05:03,030
+اللون الأحمر فتحت
+
+865
+01:05:03,030 --> 01:05:09,330
+كتكون
+
+866
+01:05:09,330 --> 01:05:14,130
+اللون الأحمر كعلى ك
+
+867
+01:05:19,880 --> 01:05:24,220
+مافي عندي اي تشققات ومافي عندي اي طبعا H2O
+
+868
+01:05:24,220 --> 01:05:28,560
+production يبقى high مش من عقل السلينتيرو بكترياسي
+
+869
+01:05:28,560 --> 01:05:33,180
+هان اتكون عندي A على
+
+870
+01:05:33,180 --> 01:05:38,420
+A اذا ملاحظين لون أصفر طبعا فوق لون أصفر تحت يبقى
+
+871
+01:05:38,420 --> 01:05:41,420
+A على A مافي عندي اي تشققات مافي عندي لون أسود
+
+872
+01:05:41,420 --> 01:05:46,700
+يبقى فقط A على A هان K فوق اتكون اللون الأحمر على
+
+873
+01:05:46,700 --> 01:05:52,870
+تحت في عندي اللون الأصفر Aمع وجود اللي هو الـ gas
+
+874
+01:05:52,870 --> 01:05:56,190
+في عندي هان تشققات يبقى في عندي gas يبقى K على A
+
+875
+01:05:56,190 --> 01:05:59,290
+with gas يبقى هاي من عقلة الـ enterobacteriasi هان
+
+876
+01:05:59,290 --> 01:06:03,610
+no change على no change يعملها بالاصل ك control
+
+877
+01:06:03,610 --> 01:06:10,010
+هاي
+
+878
+01:06:10,010 --> 01:06:12,550
+يبقى مش من عقلة الـ enterobacteriasi يبقى هاي مش
+
+879
+01:06:12,550 --> 01:06:15,750
+من عقلة الـ enterobacteriasiهي مش من عائلة ال
+
+880
+01:06:15,750 --> 01:06:18,650
+انتيرو بكترياسي لكن هي من عائلة ال انتيرو بكترياسي
+
+881
+01:06:18,650 --> 01:06:21,650
+هي من عائلة ال انتيرو بكترياسي هي من عائلة ال
+
+882
+01:06:21,650 --> 01:06:24,370
+انتيرو بكترياسي وهي كذلك هي و هي من عائلة ال
+
+883
+01:06:24,370 --> 01:06:26,890
+انتيرو بكترياسي يبقى عائلة ال انتيرو بكترياسي اما
+
+884
+01:06:26,890 --> 01:06:31,470
+تكون a على a او k على a لكن لو اتكون k على k او k
+
+885
+01:06:31,470 --> 01:06:33,950
+على not change او not change على not change يبقى
+
+886
+01:06:33,950 --> 01:06:37,790
+هي مش من عائلة ال انتيرو بكترياسي لو كانت a على a
+
+887
+01:06:37,790 --> 01:06:41,550
+يبقى هي من ال coliform lactose fermenter لكن لو
+
+888
+01:06:41,550 --> 01:06:45,470
+كانت k على a يبقى هيمن الـ Non-Lactose Fermenter
+
+889
+01:06:45,470 --> 01:06:51,050
+اللي هي من الـ Non-Colliefer هذا بالنسبة للـ S
+
+890
+01:06:51,050 --> 01:06:54,850
+اللي هو Triple Sugar Iron Agar هكذا نكون خلصنا أول
+
+891
+01:06:54,850 --> 01:06:58,890
+ميديتين الـ SIM و Triple Sugar Iron Agar أو
+
+892
+01:06:58,890 --> 01:07:02,810
+Trigger Sugar Iron Agar
+
diff --git a/PL9fwy3NUQKwZWgAb_V1ATlRRsH-6i3cPC/ssK-XRx65XI.srt b/PL9fwy3NUQKwZWgAb_V1ATlRRsH-6i3cPC/ssK-XRx65XI.srt
new file mode 100644
index 0000000000000000000000000000000000000000..fe8790aa378237f6074ed0fd0eff6aca5aa81c78
--- /dev/null
+++ b/PL9fwy3NUQKwZWgAb_V1ATlRRsH-6i3cPC/ssK-XRx65XI.srt
@@ -0,0 +1,1387 @@
+1
+00:00:01,620 --> 00:00:05,780
+فنبدأ بالجزء الثاني من الـ Chapter اللي هو يتكلم
+
+2
+00:00:05,780 --> 00:00:09,820
+عن الـ Microbial Control Agents عوامل التحكم
+
+3
+00:00:09,820 --> 00:00:14,560
+بالميكروبات. Many microorganisms are beneficial and
+
+4
+00:00:14,560 --> 00:00:18,880
+necessary for human wellbeing. قلنا أن نسبة كبيرة
+
+5
+00:00:18,880 --> 00:00:23,540
+من الميكروبات تعتبر beneficial، مفيدة ونسبة قليلة
+
+6
+00:00:23,540 --> 00:00:28,160
+منها تعتبر non-pathogenic. However, microbial
+
+7
+00:00:28,160 --> 00:00:30,740
+activities may have undesirable
+
+8
+00:00:38,540 --> 00:00:42,960
+بتسبب اللي هو تسمم الغذاء وتسبب العديد من الأمراض.
+
+9
+00:00:42,960 --> 00:00:46,140
+عشان هيك، it is essential to be able to kill
+
+10
+00:00:46,140 --> 00:00:50,420
+microorganisms or inhibit their growth to minimize
+
+11
+00:00:51,860 --> 00:00:57,080
+عشان هيك لازم يكون هنا القدرة أن احنا نقتل الـ
+
+12
+00:00:57,080 --> 00:01:00,960
+microorganisms هذه أو نعملي لها تثبيط للنمو عشان
+
+13
+00:01:00,960 --> 00:01:06,340
+نقلل هذه المخاطر. بقول لي أنه عندي أكثر من طريقة
+
+14
+00:01:06,340 --> 00:01:10,360
+للتخلص من الميكروبات. في عندي الـ physical methods، في
+
+15
+00:01:10,360 --> 00:01:14,380
+عندي الـ chemical methods، و الـ mechanical methods.
+
+16
+00:01:14,380 --> 00:01:19,520
+هذه طرق للتخلص من الميكروبات. سنتكلم عن كل طريقة
+
+17
+00:01:19,850 --> 00:01:24,630
+وفي عندي في كل طريقة طبعاً أنواع سنتكلم عنهم في الـ
+
+18
+00:01:24,630 --> 00:01:29,530
+slides القادمة. سنتكلم
+
+19
+00:01:29,530 --> 00:01:34,210
+على أول نوع من methods of microbial control، الـ
+
+20
+00:01:34,210 --> 00:01:38,210
+physical methods، الطرق الفيزيائية. الـ radiation
+
+21
+00:01:38,210 --> 00:01:42,930
+الإشعاع هو مثال على الـ physical methods. في عندي
+
+22
+00:01:42,930 --> 00:01:47,490
+أنواع من الـ radiation، من الإشعاع: الـ Ionizing
+
+23
+00:01:47,490 --> 00:01:51,390
+Radiation والـ Non-Ionizing Radiation. سنتكلم عنهم
+
+24
+00:01:51,390 --> 00:01:55,870
+بالتفصيل. الـ
+
+25
+00:01:55,870 --> 00:01:59,510
+Ionizing Radiation has deep penetration power that has
+
+26
+00:01:59,510 --> 00:02:03,610
+sufficient energy. بقول لي أنه بتمتلك قوة اختراق
+
+27
+00:02:03,610 --> 00:02:08,490
+عالية نتيجة كمية الطاقة العالية الموجودة في الإشعاع.
+
+28
+00:02:08,490 --> 00:02:12,670
+هذا يسبب to cause electrons to leave their orbits,
+
+29
+00:02:12,670 --> 00:02:17,820
+breaking DNA. بالتالي راح يطلع الإلكترون من المدار،
+
+30
+00:02:17,820 --> 00:02:23,300
+راح يخترق الحاجز ويكسر الـ DNA. هكذا أنا بما أني أنا
+
+31
+00:02:23,300 --> 00:02:27,920
+كسرت الـ DNA، تم تعطيل عملية الـ replication، التضاعف
+
+32
+00:02:27,920 --> 00:02:33,060
+في الخلية. فبالتالي، هكذا، هيؤثر على نمو الميكروب. من
+
+33
+00:02:33,060 --> 00:02:36,960
+أمثلة على الـ Ionizing Radiation: الـ Gamma-Rays،
+
+34
+00:02:36,960 --> 00:02:40,920
+الـ X-Rays، والـ Cathode-Rays. هذه الطريقة تستخدم
+
+35
+00:02:49,820 --> 00:02:53,620
+النوع الثاني من الـ radiation، الـ Non-Ionizing
+
+36
+00:02:53,620 --> 00:02:58,680
+Radiation.  He has little penetration power. بتمتلك قوة اختراق
+
+37
+00:02:58,680 --> 00:03:02,360
+قليلة لأن الطاقة التي بتمتلكها أقل، على عكس الـ
+
+38
+00:03:02,360 --> 00:03:07,170
+Ionizing التي كانت بتمتلك قوة اختراق عالية، and must
+
+39
+00:03:07,170 --> 00:03:11,090
+be directly exposed. بقول لي لازم بشكل مباشر تتعرض
+
+40
+00:03:11,090 --> 00:03:15,070
+للميكروب. في الـ ionizing radiation كان فيه  إلكترون
+
+41
+00:03:15,070 --> 00:03:20,430
+هو الذي يتعرض للميكروب، لكن هنا بشكل مباشر
+
+42
+00:03:20,430 --> 00:03:25,350
+هي نفسها الـ non-ionizing radiation هي التي تتعرض
+
+43
+00:03:25,350 --> 00:03:29,690
+للميكروب بشكل مباشر. ما بيخرج منها أيونات. مثال عليها: الـ UV light. الـ UV light بقول لي creates thymine
+
+44
+00:03:29,690 --> 00:03:34,070
+dimers.  يعني ثايمين دايمر.  لما أنا أعرض
+
+45
+00:03:34,070 --> 00:03:38,140
+البكتيريا لـ UV light، البكتيريا فيها الـ DNA،
+
+46
+00:03:38,140 --> 00:03:43,240
+المادة الوراثية. الآن المادة الوراثية طبعاً هي سلسلة
+
+47
+00:03:43,240 --> 00:03:48,780
+من القواعد النيتروجينية. فيه بيكون عندي القواعد
+
+48
+00:03:48,780 --> 00:03:52,560
+النيتروجينية الـ A والـ T بتكون رابطة ما بينهم.
+
+49
+00:03:52,560 --> 00:03:56,240
+بتكون رابطة ما بين الـ A والـ T. الآن الـ UV light لو
+
+50
+00:03:56,240 --> 00:04:01,160
+أنا عرضتها للبكتيريا، يعني للـ DNA، رح تفكك الرابطة
+
+51
+00:04:01,160 --> 00:04:07,460
+ما بين الـ A و الـ T. هتفكك الرابطة ما بين الـ A و
+
+52
+00:04:07,460 --> 00:04:11,700
+الـ T، وهتخلي الرابطة ما بين الـ T و الـ T، ورح
+
+53
+00:04:11,700 --> 00:04:16,960
+يرتبطوا ويكونوا الثايمين دايمر. هذا بالنسبة
+
+54
+00:04:16,960 --> 00:04:20,040
+لثايمين دايمر، which interferes with replication.
+
+55
+00:04:20,040 --> 00:04:24,580
+طبعاً بالتالي هي عطلت الـ DNA، عطلت عملية الـ
+
+56
+00:04:28,520 --> 00:04:33,240
+replication. فبالتالي، هتأثر على نمو الميكروب زي ما
+
+57
+00:04:33,240 --> 00:04:36,820
+احنا بنلاحظ. فيه الصورة هنا القواعد النيتروجينية
+
+58
+00:04:36,820 --> 00:04:41,120
+الـA والـT.  فأندي هنا رابطة ما بين الـA والـT.
+
+59
+00:04:41,120 --> 00:04:46,520
+لكن لما أنا عرضتها، هذا طبعاً normal DNA، لكن لما
+
+60
+00:04:46,520 --> 00:04:50,700
+أنا عرضتها لـUV، صار عندي تكون الـthymine dimer،
+
+61
+00:04:50,700 --> 00:04:54,460
+التي هي فككت الرابطة، زي ما احنا ملاحظين بين الـA
+
+62
+00:04:54,460 --> 00:04:58,780
+والـT، كونت رابطة ما بين الـT والـT، وخلتهم مع بعض.
+
+63
+00:04:58,780 --> 00:05:03,920
+هكذا الـDNA  تعطل، فبالتالي تعطل الـreplication.
+
+65
+00:05:08,810 --> 00:05:12,930
+في هذه الـ slide، نتكلم على مصطلحات مهمة. أول مصطلح:
+
+66
+00:05:12,930 --> 00:05:16,310
+الـ sterilization، التعقيم. هو معناه، في أول
+
+67
+00:05:16,310 --> 00:05:21,570
+chapter في المساق، وعرفناه أنه killing or removing
+
+68
+00:05:21,570 --> 00:05:26,370
+all forms of microbial life including endospores. هو
+
+69
+00:05:26,370 --> 00:05:31,890
+قتل كل الـ microbial، كل أشكال الـ microbial life بما
+
+70
+00:05:31,890 --> 00:05:35,960
+فيهم الـ endospores. طبعاً الـ Sterilization قلنا فيه
+
+71
+00:05:35,960 --> 00:05:38,740
+أنها أكثر من طريقة للـ Sterilization، منها الـ
+
+72
+00:05:38,740 --> 00:05:41,880
+Autoclave، الـ filtration، الـ hot oven، الـ
+
+73
+00:05:41,880 --> 00:05:45,800
+ethylene oxide. بالنسبة للـ disinfection، قلنا هي
+
+74
+00:05:45,800 --> 00:05:50,640
+عبارة عن مواد كيميائية تستخدم للـ inhibition، لتثبيط نمو
+
+75
+00:05:50,640 --> 00:05:54,460
+الكائن الحي. لكن الـ sterilization قلنا killing، الـ
+
+76
+00:05:54,460 --> 00:05:56,620
+disinfection inhibition the growth of
+
+77
+00:05:56,620 --> 00:06:00,160
+microorganisms. لكن الذي نريد أن نضيفه أن الـ
+
+78
+00:06:00,160 --> 00:06:04,280
+disinfection تتكلم على الـ non-living surfaces، أنه
+
+79
+00:06:04,280 --> 00:06:09,660
+أنا أستخدمها مواد كيميائية للجمادات، زي الطاولة. لكن
+
+80
+00:06:09,660 --> 00:06:14,120
+لو أنا أريد أن أحكي على الـ Living tissues، أستخدم مصطلح
+
+81
+00:06:14,120 --> 00:06:18,040
+الـ Antiseptic.  are chemicals used on living tissues
+
+82
+00:06:18,040 --> 00:06:20,680
+to inhibit the growth of microorganisms. يبقى الـ
+
+83
+00:06:20,680 --> 00:06:23,980
+disinfection والـ antiseptic متشابهتان، أنهما يستخدمان
+
+84
+00:06:23,980 --> 00:06:29,020
+للـinhibition، لتثبيط نمو الكائنات الحية. لكن
+
+85
+00:06:29,020 --> 00:06:33,190
+الاختلاف أنه disinfection on non-living surfaces،
+
+86
+00:06:33,190 --> 00:06:37,350
+للجمادات. لكن لو أنا أستعمل مواد كيميائية على
+
+87
+00:06:37,350 --> 00:06:41,490
+الكائنات الحية، أتحدث عن الـ Antiseptic. الـ
+
+88
+00:06:41,490 --> 00:06:45,230
+Chemotherapeutic Agent هو مصطلح شامل. أي مادة
+
+89
+00:06:45,230 --> 00:06:49,470
+كيميائية تستخدم لقتل، destroy، kill or inhibit the
+
+90
+00:06:49,470 --> 00:06:52,630
+growth of microorganisms، لكن أتحدث عن الـ Living
+
+91
+00:06:52,630 --> 00:06:59,390
+tissues. يبقى هي مصطلح عام، يستخدم لأو للتعبير عن أي
+
+92
+00:06:59,390 --> 00:07:03,370
+مادة كيميائية تستخدم لقتل أو تثبيط الكائنات الحية
+
+93
+00:07:03,370 --> 00:07:10,870
+لكن أنا أتحدث عن الـ Living tissues. في
+
+94
+00:07:10,870 --> 00:07:14,270
+الـ slide السابقة، عرفنا الـ chemotherapeutic agent،
+
+95
+00:07:14,270 --> 00:07:17,110
+التي هي المواد الكيميائية التي تستخدم لقتل أو
+
+96
+00:07:17,110 --> 00:07:22,430
+تثبيط الكائنات على in or on living tissues، في أو في
+
+97
+00:07:22,430 --> 00:07:27,360
+الـ host. لكن لما أنا أتحدث عن العملية التي تستخدم
+
+98
+00:07:27,360 --> 00:07:31,160
+فيها المواد الكيميائية، أحكي Antimicrobial
+
+99
+00:07:31,160 --> 00:07:34,520
+Chemotherapy. يبقى الـ Antimicrobial Chemotherapy
+
+100
+00:07:34,520 --> 00:07:37,620
+هي العملية is the use of chemicals، هو استخدام
+
+101
+00:07:37,620 --> 00:07:41,780
+المواد الكيميائية to inhibit or kill microorganisms
+
+102
+00:07:41,780 --> 00:07:44,960
+in or on the host. الـhost يعني الـ living tissues.
+
+103
+00:07:44,960 --> 00:07:49,220
+يبقى هذا المصطلح، والمصطلح الذي ذكرناه، آخرش الـ
+
+104
+00:07:49,220 --> 00:07:52,800
+Chemotherapeutic agent، هما تقريباً نفس المصطلح، لكن
+
+105
+00:07:52,800 --> 00:07:55,680
+الـ Chemotherapeutic Agent عبارة عن الكيميكال، اللي هي
+
+106
+00:07:55,680 --> 00:07:59,160
+المواد الكيميائية نفسها. لكن الـ Antimicrobial
+
+107
+00:07:59,160 --> 00:08:02,300
+Chemotherapy، أنا أتحدث عن العملية التي أنا أستخدم
+
+108
+00:08:02,300 --> 00:08:07,560
+فيها المواد الكيميائية لتثبيط أو قتل الـ micro
+
+109
+00:08:07,560 --> 00:08:12,320
+organisms in or on the host or living tissues. طيب، لو
+
+110
+00:08:12,320 --> 00:08:16,880
+أنا أريد أن أقسم الـ Antimicrobial Chemotherapeutic
+
+111
+00:08:16,880 --> 00:08:23,810
+Agents، نقسمهم إلى صنفين. أول صنف، اللي هو الـ Antibiotic.
+
+112
+00:08:23,810 --> 00:08:27,430
+الـ Antibiotic are substances produced as metabolic
+
+113
+00:08:27,430 --> 00:08:32,090
+products of one microorganism which inhibit or kill
+
+114
+00:08:32,090 --> 00:08:38,310
+other microorganisms. هي تؤخذ من كائن حي حتى يؤثر
+
+115
+00:08:38,310 --> 00:08:43,210
+على كائن حي آخر. آخذها من العمليات الأيضية حتى
+
+116
+00:08:43,210 --> 00:08:48,230
+يؤثر عليها من خلال التثبيط أو القتل على الـ living
+
+117
+00:08:48,230 --> 00:08:53,200
+tissues، زي ما ذكرنا. لكن بالنسبة للمصطلح الثاني، الـ
+
+118
+00:08:53,200 --> 00:08:56,540
+Antimicrobial Chemotherapeutic Chemicals. Chemicals
+
+119
+00:08:56,540 --> 00:09:01,860
+هي كيميكالز يتم إنتاجها في المختبر، in the laboratory.
+
+120
+00:09:01,860 --> 00:09:06,580
+هنا يتم إنتاجها أو يتم استخلاصها من كائنات
+
+121
+00:09:06,580 --> 00:09:10,000
+حية. لكن الـ Antimicrobial Chemotherapeutic
+
+122
+00:09:10,000 --> 00:09:14,520
+Chemicals هي مواد كيميائية يتم تصنيعها في المختبرات
+
+123
+00:09:14,520 --> 00:09:19,800
+حتى تؤثر على الـ microorganisms إما بالتثبيط أو في
+
+124
+00:09:19,800 --> 00:09:26,080
+القتل. هذا بالنسبة للفرق بينهما. بالنسبة للـ
+
+125
+00:09:26,080 --> 00:09:29,420
+Antimicrobial chemotherapy هي العملية. لكن لما
+
+126
+00:09:29,420 --> 00:09:34,020
+نتكلم على الـ chemicals نفسها، نقول الـ chemotherapeutic
+
+127
+00:09:34,020 --> 00:09:39,680
+agents. الـ Chemotherapeutic Agents يصنفان إلى صنفين
+
+128
+00:09:39,680 --> 00:09:42,540
+اللذان هما الـ Antibiotics وAntimicrobial
+
+129
+00:09:42,540 --> 00:09:46,100
+Chemotherapeutic Chemicals. الـ Antibiotics هي مواد
+
+130
+00:09:46,100 --> 00:09:50,440
+من كائنات حية، تستخلص من كائن حي. لكن الـ
+
+131
+00:09:50,440 --> 00:09:55,000
+Antimicrobial Chemotherapeutic Chemicals يتم
+
+132
+00:09:55,000 --> 00:09:56,760
+تصنيعها في المختبر.
+
+133
+00:10:00,140 --> 00:10:03,300
+في الـ slide السابقة، صنفنا الـ Antimicrobial
+
+134
+00:10:03,300 --> 00:10:07,720
+Agents حسب الـ origin، حسب المصدر: إلى Antibiotics و
+
+135
+00:10:07,720 --> 00:10:11,040
+Antimicrobial Chemotherapeutic Chemicals. لأنه قلنا
+
+136
+00:10:11,040 --> 00:10:14,740
+أن الـ Antibiotics تستخلص من كائن حي. لكن الـ
+
+137
+00:10:14,740 --> 00:10:18,560
+Antimicrobial Chemotherapeutic Chemicals كانت من
+
+138
+00:10:18,560 --> 00:10:22,680
+المختبر، يتم تصنيعها في المختبر. الآن نصنف الـ
+
+139
+00:10:22,680 --> 00:10:27,120
+Antimicrobial Agents حسب الـ action، حسب تأثيرها.
+
+140
+00:10:27,120 --> 00:10:32,750
+تصنف إلى اثنين: cidal. ماذا تعني cidal؟ cidal اللي
+
+141
+00:10:32,750 --> 00:10:36,590
+هو الذي يقتل microorganisms، التي تقتل الـ microorganisms.
+
+142
+00:10:36,590 --> 00:10:40,090
+لأننا اتفقنا أن الـ antimicrobial agents
+
+143
+00:10:40,090 --> 00:10:45,230
+مصطلح عام يستخدم لقتل أو تثبيط. فلو كان الـ action
+
+144
+00:10:45,230 --> 00:10:49,510
+cidal، يبقى أنا أتحدث عن قتل الـ microorganisms. في
+
+145
+00:10:49,510 --> 00:10:54,210
+عندي أمثلة على antibiotics تستخدم في قتل الـ microorganisms:
+
+146
+00:10:54,210 --> 00:10:58,550
+البنسلين، السيفالوسبورين، الستربتوميسين،
+
+147
+00:10:58,550 --> 00:11:03,790
+النيوميسين. هذا بالنسبة لـ cidal. بالنسبة للـ static،
+
+148
+00:11:03,790 --> 00:11:07,430
+التي هي Inhibit microbial growth long enough for
+
+149
+00:11:07,430 --> 00:11:10,830
+the body's own defenses to remove the organisms.
+
+150
+00:11:10,830 --> 00:11:16,310
+تعمل على تثبيط نمو الكائن الحي. من الأمثلة على الـ
+
+151
+00:11:16,310 --> 00:11:20,350
+Antibiotics أو الـ Antimicrobial agents التي هي static
+
+152
+00:11:20,350 --> 00:11:25,950
+in action: Tetracycline، erythromycin، sulfonamide.
+
+153
+00:11:26,090 --> 00:11:30,270
+هذه كلها Antimicrobial Agents.  بالنسبة لـ Action
+
+154
+00:11:30,270 --> 00:11:34,070
+static، تعمل Inhibition للميكروبية للجراثيم. لكن
+
+155
+00:11:34,070 --> 00:11:37,810
+بالنسبة لـ Antimicrobial Agents التي تعمل قتل،
+
+156
+00:11:37,810 --> 00:11:41,330
+cidal in action: البنسلين، Cephalosporin،
+
+157
+00:11:41,330 --> 00:11:45,890
+Streptomycin، Neomycin. هذه كلها حفظ، مطلوب أنكم
+
+158
+00:11:45,890 --> 00:11:50,130
+تحفظوا:  ما هي الـ Antimicrobial Agents التي تعمل cidal
+
+159
+00:11:50,130 --> 00:11:53,840
+in action، ومن التي تعمل static in action؟  طب نأتي
+
+160
+00:11:53,840 --> 00:11:58,140
+لمصطلح مهم، وهو selective toxicity. Selective
+
+161
+00:11:58,140 --> 00:12:02,720
+toxicity. ماذا يعني هذا المصطلح؟ معناه السمية الانتقائية.
+
+162
+00:12:02,720 --> 00:12:07,360
+يعني أن that the agent used must inhibit or kill
+
+163
+00:12:07,360 --> 00:12:11,440
+the microorganism without seriously harming the
+
+164
+00:12:11,440 --> 00:12:16,480
+host. بقول لي أن الـ chemicals أو الـ antimicrobial
+
+165
+00:12:16,480 --> 00:12:19,380
+agents التي تعمل inhibition or kill  للـ micro
+
+166
+00:12:19,380 --> 00:12:24,170
+organisms، لازم أنه يعمل هذا الـ action، يثبت أو يقتل
+
+167
+00:12:24,170 --> 00:12:28,290
+الميكروب من غير ما يؤثر على الـ host، على العائل
+
+168
+00:12:28,290 --> 00:12:33,290
+بأي ضرر. هذا بالنسبة لـ Selective Toxicity، أنه
+
+169
+00:12:33,290 --> 00:12:37,670
+الـ Antimicrobial Agent لازم يثبت أو يقتل الميكروب
+
+170
+00:12:37,670 --> 00:12:43,310
+من غير ما يؤثر على العائل بأي ضرر. طبيعي طبعاً أنه
+
+171
+00:12:43,310 --> 00:12:47,690
+يكون فيه side effects، آثار جانبية، لكن لازم أنها تكون
+
+172
+00:12:47,690 --> 00:12:51,630
+غير مؤذية للعائل. هذا بالنسبة لتصنيف الـ
+
+173
+00:12:51,630 --> 00:12:58,490
+Antimicrobial Agents حسب الـ Action. قلنا
+
+174
+00:12:58,490 --> 00:13:02,190
+أن الـ Antimicrobial Agents تصنف حسب أول شيء الـ
+
+175
+00:13:02,190 --> 00:13:05,930
+Origin: إلى Antibiotics و Antimicrobial chemotherapeutic
+
+176
+00:13:05,930 --> 00:13:10,710
+chemicals. حسب الـ Action: إلى cidal و static. حسب، الآن
+
+177
+00:13:10,710 --> 00:13:14,950
+نأتي لـ Spectrum، حسب المادة أو المجال الذي يؤثر
+
+178
+00:13:14,950 --> 00:13:19,320
+عليه. هي الـ Antimicrobial agents تصنف إلى Broad
+
+179
+00:13:19,320 --> 00:13:23,580
+Spectrum و Narrow Spectrum. بالنسبة لـ Broad
+
+180
+00:13:23,580 --> 00:13:28,680
+Spectrum، الذي
+
+223
+00:16:23,520 --> 00:16:27,840
+هيعمل إنه هيكون أقل ضرر لبكتيريا الـ normal flora
+
+224
+00:16:27,840 --> 00:16:32,860
+هاي بالتالي هيحافظ عليها، هتظل تعمل الوظائف
+
+225
+00:16:32,860 --> 00:16:37,560
+المختلفة لها. طيب، هنكتب لأن سوء استخدام أو
+
+226
+00:16:37,560 --> 00:16:41,200
+استخدام للـ broad-spectrum antibiotic can lead to
+
+227
+00:16:41,200 --> 00:16:43,840
+a superinfection. بيقود لـ super infection by
+
+228
+00:16:43,840 --> 00:16:46,740
+opportunistic microorganism. شو يعني super
+
+229
+00:16:46,740 --> 00:16:50,400
+infection يعني؟ نتيجة خلل معين، على سبيل المثال،
+
+230
+00:16:50,400 --> 00:16:54,500
+استخدام Antibiotic/ Antimicrobial Agent Broad
+
+231
+00:16:54,500 --> 00:16:58,780
+Spectrum راح تقضي على الـ normal flora وعلى
+
+232
+00:16:58,780 --> 00:17:02,640
+الميكروب. راح تيجي البكتيريا الانتهازية وتسبب له
+
+233
+00:17:02,640 --> 00:17:06,300
+عدوى. هذه العدوى بسميها Super Infection، لأن هو
+
+234
+00:17:06,300 --> 00:17:11,180
+بالأساس ما كان له عدوى، لكن نتيجة اللي هو استخدام
+
+235
+00:17:11,180 --> 00:17:15,120
+الـ broad-spectrum antimicrobial agent، صار هذا
+
+236
+00:17:15,120 --> 00:17:18,970
+الأمر. لكن لو أنا بتكلم على الـ primary infection،
+
+237
+00:17:18,970 --> 00:17:23,990
+بتكون العدوى ابتدائية من، طبعا، من بكتيريا ممرضة،
+
+238
+00:17:23,990 --> 00:17:28,810
+وبتسبب المرض. هذا بالنسبة لآخر تصنيف من تصنيفات الـ
+
+239
+00:17:28,810 --> 00:17:32,610
+antimicrobial agent حسب الـ spectrum. ومطلوب حفظ
+
+240
+00:17:32,610 --> 00:17:35,530
+اللي هو الأمثلة على الـ broad spectrum و narrow
+
+241
+00:17:35,530 --> 00:17:39,750
+spectrum. وآخر شيء لازم نعرف ليش أنا، أو شو بفضل أكيد
+
+242
+00:17:39,750 --> 00:17:42,990
+بنفضل الـ narrow spectrum لأنه more specific. ثاني
+
+243
+00:17:42,990 --> 00:17:46,870
+شيء، لأنه will cause less destruction to the
+
+244
+00:17:46,870 --> 00:17:50,830
+body's normal flora. لو أنا استخدمت الـ broad
+
+245
+00:17:50,830 --> 00:17:53,990
+spectrum antimicrobial agent، هيسبب لي super
+
+246
+00:17:53,990 --> 00:17:58,150
+infection by opportunistic microorganism. وعرفنا شو
+
+247
+00:17:58,150 --> 00:18:01,710
+يعني super infection، وشو يعني primary infection.
+
+248
+00:18:05,420 --> 00:18:09,280
+في هاي الـ slide، هنتكلم على مصطلحات مهمة. هو
+
+249
+00:18:09,280 --> 00:18:13,280
+المصطلح الـ antibiotic-resistant، اللي هي مقاومة
+
+250
+00:18:13,280 --> 00:18:18,160
+المضادات الحيوية. bacteria gain ability to grow no
+
+251
+00:18:18,160 --> 00:18:22,760
+longer sensitive to a drug. إنه البكتيريا بيصير
+
+252
+00:18:22,760 --> 00:18:27,460
+عندها القدرة إنها تنمو حتى بوجود المضاد الحيوي.
+
+253
+00:18:27,460 --> 00:18:31,860
+بطلت تتأثر في الـ antimicrobial agent.
+
+254
+00:18:33,080 --> 00:18:37,400
+Antiretroviral هي عبارة عن مضادات بتشتغل ضد
+
+255
+00:18:37,400 --> 00:18:41,560
+الفيروسات. طب نيجي لـ combination of drugs، اللي
+
+256
+00:18:41,560 --> 00:18:45,260
+هو اتحاد الأدوية. فيه عندي تلات مصطلحات مهمة بالنسبة
+
+257
+00:18:45,260 --> 00:18:49,960
+لـ combination of drugs: synergism، antagonism، و
+
+258
+00:18:49,960 --> 00:18:54,820
+additive. بالنسبة لأول واحد اللي هو synergism،
+
+259
+00:18:54,820 --> 00:18:58,860
+synergism يعني action of two antibiotics greater
+
+260
+00:18:58,860 --> 00:19:04,630
+than the sum of their individual effects. مجموع تأثير/فعالية المضادات الحيوية لإثنين أكبر من
+
+261
+00:19:04,630 --> 00:19:10,070
+تأثير كل مضاد حيوي على حدة. يعني واحد زائد واحد
+
+262
+00:19:10,070 --> 00:19:13,350
+بيكون مش بيساوي اتنين، أكبر من اتنين، لأن مجموع
+
+263
+00:19:13,350 --> 00:19:17,810
+تأثير فعاليتهم مع بعض أكبر من تأثير كل مضاد حيوي
+
+264
+00:19:17,810 --> 00:19:22,650
+على حدة. فبالتالي، في هاي الحالة، منيح إنه ناخد هدول
+
+265
+00:19:22,650 --> 00:19:27,230
+الأدوية مع بعض، لأن تأثيرهم مع بعض أكبر من تأثير كل
+
+266
+00:19:27,230 --> 00:19:31,980
+مضاد حيوي على حدة. بالنسبة لـ antagonism، اللي هو
+
+267
+00:19:31,980 --> 00:19:36,080
+action of two drugs is reduced; less effective. إنه مجموع
+
+268
+00:19:36,080 --> 00:19:40,060
+تأثير الـ two drugs مع بعض أقل من تأثير كل واحد على
+
+269
+00:19:40,060 --> 00:19:45,220
+حدا. يعني واحد زائد واحد بيكون أقل من اتنين. أخر
+
+270
+00:19:45,220 --> 00:19:49,300
+شيء هي الـ additive: the action of two drugs in
+
+271
+00:19:49,300 --> 00:19:52,140
+combination is equal to the sum of their
+
+272
+00:19:52,140 --> 00:19:55,540
+independent activity when studied separately. بقول
+
+273
+00:19:55,540 --> 00:19:59,880
+لي إن مجموع تأثير المضادين الحيويين بيساوي تأثير
+
+274
+00:19:59,880 --> 00:20:03,680
+كل مضاد حيوي على حدة. يعني واحد زائد واحد بيساوي
+
+275
+00:20:03,680 --> 00:20:08,220
+اتنين. هذا بالنسبة لـ combination of drugs. نيجي
+
+276
+00:20:08,220 --> 00:20:12,180
+لمصطلحين مهمين، اللي هما الـ minimum inhibitory
+
+277
+00:20:12,180 --> 00:20:18,200
+concentration (MIC). The lowest concentration of
+
+278
+00:20:18,200 --> 00:20:22,120
+chemotherapy agent capable of preventing growth of
+
+279
+00:20:22,120 --> 00:20:27,140
+the test organism. هو أقل تركيز من الـ antimicrobial
+
+280
+00:20:27,140 --> 00:20:31,740
+agent قادر على منع نمو البكتيريا. شو منع نمو
+
+281
+00:20:31,740 --> 00:20:34,900
+البكتيريا؟ شو معناها؟ اللي هي التثبيط، inhibition.
+
+282
+00:20:34,900 --> 00:20:39,200
+عشان هيك أنا قلت minimum inhibitory، يعني هو أقل
+
+283
+00:20:39,200 --> 00:20:42,160
+تركيز من الـ antimicrobial agent قادر إنه يعمل
+
+284
+00:20:42,160 --> 00:20:46,200
+inhibition للبكتيريا. Preventing the growth يعني
+
+285
+00:20:46,200 --> 00:20:50,640
+يعمل inhibition. لكن الـ minimum bactericidal
+
+286
+00:20:50,640 --> 00:20:54,650
+concentration، اللي هو the lowest concentration of
+
+287
+00:20:54,650 --> 00:20:59,850
+chemotherapy agent whose result is no growth، اللي
+
+288
+00:20:59,850 --> 00:21:05,230
+هي turbidity of subculture. هو أقل تركيز من الـ
+
+289
+00:21:05,230 --> 00:21:09,830
+antibiotic أو الـ antimicrobial agent قادر على قتل
+
+290
+00:21:09,830 --> 00:21:12,770
+البكتيريا. إنه يكون فيه عندي no growth. No growth
+
+291
+00:21:12,770 --> 00:21:17,030
+يعني قتل البكتيريا، إن ما يكون فيه عندي بكتيريا في
+
+292
+00:21:17,030 --> 00:21:21,940
+الـ culture. أو ممكن نحكي في تعريف أدق، اللي هو أقل
+
+293
+00:21:21,940 --> 00:21:26,280
+تركيز من الـ antimicrobial agent بيسمح بنمو أقل من
+
+294
+00:21:26,280 --> 00:21:31,520
+1 من 100% من الميكروب. هذا بالنسبة لـ minimum
+
+295
+00:21:31,520 --> 00:21:34,720
+inhibitory concentration والـ minimum bactericidal
+
+296
+00:21:34,720 --> 00:21:38,380
+concentration. الفصل الجاي في مساق الـ diagnostic
+
+297
+00:21:38,380 --> 00:21:41,780
+medical microbiology، هتتعرفوا بالتفصيل كيف أنا بدي
+
+298
+00:21:41,780 --> 00:21:46,020
+أحسب الـ minimum inhibitory concentration والـ
+
+299
+00:21:46,020 --> 00:21:48,300
+minimum bactericidal concentration.
+
+300
+00:21:53,540 --> 00:21:58,120
+Antimicrobial activity. كيف تأثير أو كيف بيأثر الـ
+
+301
+00:21:58,120 --> 00:22:02,580
+antimicrobial agent على الخلية. في عندي خمس طرق of
+
+302
+00:22:02,580 --> 00:22:08,680
+antimicrobial activity. هنذكرهم بشكل مختصر. الفصل
+
+303
+00:22:08,680 --> 00:22:11,520
+الجاي في مادة الـ diagnostic medical microbiology
+
+304
+00:22:11,520 --> 00:22:16,300
+هيكون فيه chapter كامل على هذا الموضوع. يعني هيكون
+
+305
+00:22:16,300 --> 00:22:21,530
+فيه تفاصيل أكثر. لكن في هذا الفصل فقط، هنذكر بعض
+
+306
+00:22:21,530 --> 00:22:26,450
+المعلومات الأساسية. بالنسبة لأول طريقة: injury to
+
+307
+00:22:26,450 --> 00:22:29,490
+plasma membrane. ممكن أن الـ antimicrobial agent
+
+308
+00:22:29,490 --> 00:22:32,050
+يؤثر على الـ plasma membrane. مثال على
+
+309
+00:22:32,050 --> 00:22:36,330
+antimicrobial agent: الـ polymyxin B. هو هذا بيؤثر
+
+310
+00:22:36,330 --> 00:22:39,730
+على الـ plasma membrane. ممكن أنه يعمل inhibition of
+
+311
+00:22:39,730 --> 00:22:43,850
+cell wall synthesis. مثال عليه: الـ penicillin و الـ
+
+312
+00:22:43,850 --> 00:22:48,650
+bacitracin. وإحنا قولنا في أول معامل في المادة إن
+
+313
+00:22:48,650 --> 00:22:51,810
+الـ penicillin بيعمل على تفكيك الرابطة اللي كانت
+
+314
+00:22:51,810 --> 00:22:55,610
+بين الـ bacterial glycan. فبالتالي بيعمل inhibition
+
+315
+00:22:55,610 --> 00:22:59,470
+للـ cell wall synthesis. ممكن يؤثر على الـ protein
+
+316
+00:22:59,470 --> 00:23:03,950
+synthesis، بالتحديد اللي هي الـ translation. ممكن
+
+317
+00:23:03,950 --> 00:23:07,030
+يؤثر، يعمل inhibition of nucleic acid replication
+
+318
+00:23:07,030 --> 00:23:10,930
+أو transcription. ممكن يعمل inhibition of
+
+319
+00:23:10,930 --> 00:23:13,950
+essential metabolites. هذا بالنسبة للـ
+
+320
+00:23:13,950 --> 00:23:19,290
+antimicrobial activity. بالنسبة
+
+321
+00:23:19,290 --> 00:23:21,950
+للـ antimicrobial activity، قولنا ممكن أنه يعمل
+
+322
+00:23:21,950 --> 00:23:25,870
+injury للـ plasma membrane، مثلًا عليه الـ بولي
+
+323
+00:23:25,870 --> 00:23:30,410
+ميكسين ب. ممكن يعمل inhibition للـ cell wall
+
+324
+00:23:30,410 --> 00:23:32,990
+synthesis. قولنا في أمثلة: الـ penicillin،
+
+325
+00:23:32,990 --> 00:23:36,770
+الـ cephalosporin، الـ bacitracin، الـ vancomycin. ممكن يعمل
+
+326
+00:23:36,770 --> 00:23:40,170
+inhibition لـ protein synthesis. مثال عليه، بالأخص
+
+327
+00:23:40,170 --> 00:23:43,890
+اللي هو translation: الـ chloramphenicol، الـ
+
+328
+00:23:43,890 --> 00:23:47,470
+erythromycin، الـ tetracycline، الـ streptomycin. ممكن
+
+329
+00:23:47,470 --> 00:23:52,230
+يؤثر على اللي هو inhibition of synthesis of
+
+330
+00:23:52,230 --> 00:23:56,990
+essential metabolites. ممكن يؤثر على اللي هو لـ
+
+331
+00:23:56,990 --> 00:24:00,450
+nucleic acid replication and transcription. طبعًا
+
+332
+00:24:00,450 --> 00:24:04,130
+إحنا بس بنعرف اللي هو شو الـ active، شو الـ
+
+333
+00:24:04,130 --> 00:24:07,740
+antimicrobial activity. الأمثلة في هاي الـ slide مش
+
+334
+00:24:07,740 --> 00:24:12,880
+مطلوب حفظها. الفصل الجاي هتكون المادة بالتفصيل، لكن
+
+335
+00:24:12,880 --> 00:24:18,860
+إحنا في هذا الفصل بس بدنا نعرف شو الطرق، أو كيف الـ
+
+336
+00:24:18,860 --> 00:24:24,060
+antimicrobial agent بيؤثر على الخلية البكتيرية.
+
+337
+00:24:24,060 --> 00:24:28,180
+مطلوب فقط حفظ اللي تم ذكره في الـ slide اللي قبل
+
+338
+00:24:28,180 --> 00:24:33,030
+فقط. أما في هاي الـ slide، فقط بدنا نعرف شو طرق، بشكل
+
+339
+00:24:33,030 --> 00:24:37,290
+مختصر، كيف بيأثر الـ antimicrobial agent على الخلية
+
+340
+00:24:37,290 --> 00:24:41,850
+البكتيرية بالقتل أو بالتثبيط، بدون حفظ الأمثلة. لكن
+
+341
+00:24:41,850 --> 00:24:49,610
+مطلوب حفظ ما تم ذكره في الـ slide اللي قبل. How
+
+342
+00:24:49,610 --> 00:24:53,370
+to choose antimicrobial drug. عوامل لازم أخذها بعين
+
+343
+00:24:53,370 --> 00:24:58,770
+الاعتبار قبل وصف أي antimicrobial drug. هل هو narrow
+
+344
+00:24:58,770 --> 00:25:02,970
+spectrum أو broad spectrum؟ قلنا يفضل إن أنا أستخدم
+
+345
+00:25:02,970 --> 00:25:06,190
+الـ narrow spectrum، للأسباب اللي ذكرناها سابقًا.
+
+346
+00:25:06,190 --> 00:25:10,430
+ممكن إننا نستخدم الـ broad spectrum في حالات
+
+347
+00:25:10,430 --> 00:25:15,770
+استثنائية. هل المريض old age أو child؟ نعرف إن الـ
+
+348
+00:25:15,770 --> 00:25:19,730
+antibiotic فيه تركيزات مختلفة. فأكيد مش هعطي الـ
+
+349
+00:25:19,730 --> 00:25:23,810
+child نفس تركيز الـ old age. male أو female،
+
+350
+00:25:23,810 --> 00:25:29,600
+pregnant أو lactating women. inpatient أو outpatient.
+
+351
+00:25:29,600 --> 00:25:34,360
+inpatient، اللي هو مريض داخل المشفى، بيصرف له broad
+
+352
+00:25:34,360 --> 00:25:38,680
+spectrum، لأنه بيكون معرض للتعرض للكثير من
+
+353
+00:25:38,680 --> 00:25:44,480
+الميكروبات. لكن الـ outpatient، اللي هو خارج المشفى،
+
+354
+00:25:44,480 --> 00:25:49,200
+type of microorganism، نوع الـ microorganism. site
+
+355
+00:25:49,200 --> 00:25:54,730
+of infection، اللي هي مكان العدوى. ممكن تكون سطحية،
+
+356
+00:25:54,730 --> 00:26:01,230
+فبيتم صرف مضاد حيوي على شكل مرهم. لكن لو كان داخل
+
+357
+00:26:01,230 --> 00:26:06,890
+الجسم، بيتم إعطاؤه capsule أو injection. هذا بالنسبة
+
+358
+00:26:06,890 --> 00:26:10,970
+للعوامل اللي لازم أخذها بعين الاعتبار عند وصف أي
+
+359
+00:26:10,970 --> 00:26:18,650
+مضاد حيوي. هاي
+
+360
+00:26:18,650 --> 00:26:22,840
+الـ slide من chapter الـ serial dilution. حابب أنوه
+
+361
+00:26:22,840 --> 00:26:27,280
+لبعض المعلومات اللي ما ذكرتها في الـ chapter هلأ.
+
+362
+00:26:27,280 --> 00:26:31,080
+بالنسبة للـ pour plate، شو قصده في الـ pour plate؟
+
+363
+00:26:31,080 --> 00:26:35,280
+الـ pour plate، إنه أنا بحط العينة قبل ما أنا أصب
+
+364
+00:26:35,280 --> 00:26:40,280
+الميديا. هيك أنا بوفر ظروف لا هوائية. لكن الـ spread
+
+365
+00:26:40,280 --> 00:26:44,400
+plate، الـ spread plate، بحط بتكون مصبوبة الميديا،
+
+366
+00:26:44,400 --> 00:26:48,880
+بعدين بحط العينة. هيك أنا بوفر ظروف هوائية. طيب، ليش
+
+367
+00:26:48,880 --> 00:26:50,660
+في الـ slide ذاكرها؟
+
+368
+00:26:53,960 --> 00:26:58,420
+10<sup>3</sup>، وأخذ من هون اللي هو من الـ
+
+369
+00:26:58,420 --> 00:27:01,780
+dilution. 10<sup>3</sup>، هون أخذ من الـ dilution
+
+370
+00:27:01,780 --> 00:27:06,000
+أو من الـ tube التخفيف تبعها. 10<sup>2</sup>،
+
+371
+00:27:06,000 --> 00:27:10,180
+وذكر 10<sup>3</sup>. إحنا قولنا في القانون الـ
+
+372
+00:27:10,180 --> 00:27:14,460
+concentration، اللي هو الـ number of colonies، عدد الـ
+
+373
+00:27:14,460 --> 00:27:20,040
+colonies اللي موجودة على الطبق، على الـ S، اللي هي الـ
+
+374
+00:27:20,040 --> 00:27:24,110
+volume اللي أنا أخذته من هااي التوب، وفرّدتُه أو
+
+375
+00:27:24,110 --> 00:27:29,990
+حطيته على الـ plate، ضرب الـ dilution. هذا الرقم هو
+
+376
+00:27:29,990 --> 00:27:35,590
+حاصل ضرب الـ dilution مع الـ S. الـ S ضرب اللي هو
+
+377
+00:27:35,590 --> 00:27:42,390
+الـ dilution. هذا الرقم. هون أخذت 1 مل من هااي
+
+378
+00:27:42,390 --> 00:27:45,710
+التيوب. فأنا ضربتها في تخفيفها، اللي هي 10<sup>3</sup>،
+
+379
+00:27:45,710 --> 00:27:51,300
+فطلع 10<sup>3</sup>. لكن هون أخذت 1 من
+
+380
+00:27:51,300 --> 00:27:58,000
+10 مل، ضرب اللي هي الـ 10<sup>2</sup>، فطلعت
+
+381
+00:27:58,000 --> 00:28:01,960
+الـ 10<sup>3</sup>. هذا بالنسبة للرقم. ليش هون مكتوب
+
+382
+00:28:01,960 --> 00:28:06,140
+10<sup>3</sup>؟ هي الرقم هذا حاصل ضرب الـ dilution
+
+383
+00:28:06,140 --> 00:28:11,160
+مع الـ S. هون أخذت 1 من 10، لكن هون أخذت 1 مل.
+
+384
+00:28:11,160 --> 00:28:17,960
+لازم يكون الرقم اللي هو الـ S لازم يكون بالـ مل. لو
+
+385
+00:28:17,960 --> 00:28:23,220
+كان في الـ µl، لازم أحوله لـ مل. هذه معلومة مهمة
+
+386
+00:28:23,220 --> 00:28:30,400
+لازم ننتبه لها في الامتحانات. هيك
+
+387
+00:28:30,400 --> 00:28:35,680
+بنكون وصلنا لنهاية شرح المحاضرة، وأيضًا لنهاية سلسلة
+
+388
+00:28:35,680 --> 00:28:40,040
+محاضرات مساق الـ essential medical microbiology.
+
+389
+00:28:40,040 --> 00:28:45,180
+أتمنى لكم كل التوفيق والنجاح. والسلام عليكم ورحمة
+
+390
+00:28:45,180 --> 00:28:46,400
+الله وبركاته.
diff --git a/PL9fwy3NUQKwZWgAb_V1ATlRRsH-6i3cPC/ssK-XRx65XI_postprocess.srt b/PL9fwy3NUQKwZWgAb_V1ATlRRsH-6i3cPC/ssK-XRx65XI_postprocess.srt
new file mode 100644
index 0000000000000000000000000000000000000000..36381d534d612d8ce88e652f74bb3a1c4c1fa732
--- /dev/null
+++ b/PL9fwy3NUQKwZWgAb_V1ATlRRsH-6i3cPC/ssK-XRx65XI_postprocess.srt
@@ -0,0 +1,1560 @@
+1
+00:00:01,620 --> 00:00:05,780
+فنبدأ بالجزء التاني من الـ Chapter اللي هو بتكلم
+
+2
+00:00:05,780 --> 00:00:09,820
+عن الـ Microbial Control Agent عوامل التحكم
+
+3
+00:00:09,820 --> 00:00:14,560
+بالميكروبات Many microorganisms are beneficial and
+
+4
+00:00:14,560 --> 00:00:18,880
+necessary for human wellbeing قولنا إن نسبة كبيرة
+
+5
+00:00:18,880 --> 00:00:23,540
+من الميكروبات تعتبر beneficial، مفيدة ونسبة قليلة
+
+6
+00:00:23,540 --> 00:00:28,160
+منها تعتبر non-pathogenic however, microbial
+
+7
+00:00:28,160 --> 00:00:30,740
+activities may have undesirable
+
+8
+00:00:38,540 --> 00:00:42,960
+بتسبب اللي هو تسامم الغذاء وتسبب العديد من الأمراض
+
+9
+00:00:42,960 --> 00:00:46,140
+عشان هيك it is essential to be able to kill
+
+10
+00:00:46,140 --> 00:00:50,420
+microorganism or inhibit their growth to minimize
+
+11
+00:00:51,860 --> 00:00:57,080
+عشان هيك لازم يكون هنا القدرة ان احنا نقتل ال
+
+12
+00:00:57,080 --> 00:01:00,960
+micro organism هاي او نعملي لها تثبيت لانمو عشان
+
+13
+00:01:00,960 --> 00:01:06,340
+نقلل هاي المخاطر بقوللي انه عندي اكتر من طريقة
+
+14
+00:01:06,340 --> 00:01:10,360
+للتخلص من الميكروبات في عندي ال physical method في
+
+15
+00:01:10,360 --> 00:01:14,380
+عندي ال chemical method و ال mechanical method
+
+16
+00:01:14,380 --> 00:01:19,520
+هيكلها طرق للتخلص من الميكروبات هنتكلم عن كل طريقة
+
+17
+00:01:19,850 --> 00:01:24,630
+وفي عندي في كل طريقة طبعا أنواع هنتكلم عنهم في ال
+
+18
+00:01:24,630 --> 00:01:29,530
+slides اللي جايه هنتكلم
+
+19
+00:01:29,530 --> 00:01:34,210
+على أول نوع من ال method of microbial control ال
+
+20
+00:01:34,210 --> 00:01:38,210
+physical method الطرق الفيزيائية ال radiation
+
+21
+00:01:38,210 --> 00:01:42,930
+الإشعاع هو مثال على ال physical method في عندي
+
+22
+00:01:42,930 --> 00:01:47,490
+أنواع من ال radiation من الإشعاعالـ Ionizing
+
+23
+00:01:47,490 --> 00:01:51,390
+Radiation والـ Non-Ionizing Radiation هنتكلم عنهم
+
+24
+00:01:51,390 --> 00:01:55,870
+بالتفصيل الـ
+
+25
+00:01:55,870 --> 00:01:59,510
+Ionizing Radiation deep penetration power that has
+
+26
+00:01:59,510 --> 00:02:03,610
+sufficient energy بقوللي أنه بتمتلك قوة اختراق
+
+27
+00:02:03,610 --> 00:02:08,490
+عالية نتيجة كمية الطاقة العالية الموجودة بالإشعار
+
+28
+00:02:08,490 --> 00:02:12,670
+هذا هيسبب to cause electron to leave their orbit
+
+29
+00:02:12,670 --> 00:02:17,820
+breaks DNAبالتالي راح يطلع عند الإلكترون من المدار
+
+30
+00:02:17,820 --> 00:02:23,300
+راح يخترق الحاجز ويكسر الـ DNA هك أنا بما أني أنا
+
+31
+00:02:23,300 --> 00:02:27,920
+كسرت الـ DNA تم تعطيل عملية الـ replication التطاع
+
+32
+00:02:27,920 --> 00:02:33,060
+في الخلية فبالتالي هك هيأثر على نمو الميكروب من
+
+33
+00:02:33,060 --> 00:02:36,960
+الأمثلة على الـ Ionizing Radiation الـ Gamma-Rays
+
+34
+00:02:36,960 --> 00:02:40,920
+الـ X-Rays والـ Cathode-Rays هذه الطريقة تستخدم
+
+35
+00:02:49,820 --> 00:02:53,620
+النوع التاني من الرادياشن الـ Non-Ionizing
+
+36
+00:02:53,620 --> 00:02:58,680
+Radiation هفليتل بنترياشن بوار بتمتلك قوة اختراق
+
+37
+00:02:58,680 --> 00:03:02,360
+قليلة لأن الطاقة اللي بتمتلكها أقل على عكس ال
+
+38
+00:03:02,360 --> 00:03:07,170
+Ionizing اللي كانت بتمتلك قوة اختراق عاليةand must
+
+39
+00:03:07,170 --> 00:03:11,090
+be directly exposed بقولي لازم بشكل مباشر تتعرض
+
+40
+00:03:11,090 --> 00:03:15,070
+لالميكروب في ال ionizing radiation كان فيه أندي
+
+41
+00:03:15,070 --> 00:03:20,430
+إلكترون هو اللي يتعرض لالميكروب لكن هان بشكل مباشر
+
+42
+00:03:20,430 --> 00:03:25,350
+هي نفسها ال non-ionizing radiation هي اللي هتتعرض
+
+43
+00:03:25,350 --> 00:03:29,690
+لالميكروب بشكل مباشر ما بيخرج منها ايونات نطال
+
+44
+00:03:29,690 --> 00:03:34,070
+عليها ال UV light ال UV light بقولي creates تايمان
+
+45
+00:03:34,070 --> 00:03:38,140
+ديامر أش يعني تايمان ديامرهلأ لما أنا أعرض
+
+46
+00:03:38,140 --> 00:03:43,240
+البكتيريا لـ UV light البكتيريا فيها الـ DNA
+
+47
+00:03:43,240 --> 00:03:48,780
+المادة الوراثية هلأ المادة الوراثية طبعا هي سلسلة
+
+48
+00:03:48,780 --> 00:03:52,560
+من القواعد النيتروجينية فيه بيكون عندي القاعد
+
+49
+00:03:52,560 --> 00:03:56,240
+النيتروجينية الـ A والـ T بتكون رابط ما بينهم
+
+50
+00:03:56,240 --> 00:04:01,160
+بتكون رابط ما بين الـ A والـ T هلأ الـ UV light لو
+
+51
+00:04:01,160 --> 00:04:07,460
+أنا عرضتها للبكتيريا يعني للـ DNAرح تفكك الرابطة
+
+52
+00:04:07,460 --> 00:04:11,700
+ما بين الـ A و الـ T هتفكك الرابطة ما بين الـ A و
+
+53
+00:04:11,700 --> 00:04:16,960
+الـ T و هتخلي الرابطة ما بين الـ T و الـ T و رح
+
+54
+00:04:16,960 --> 00:04:20,040
+يرتبطوا و يكونوا الثيامان ديامر هذا بالنسبة
+
+55
+00:04:20,040 --> 00:04:24,580
+لثيامان ديامر which interfere with replication
+
+56
+00:04:24,580 --> 00:04:28,520
+طبعا بالتالي هي عطلت الـ DNA عطلت عملية الـ
+
+57
+00:04:28,520 --> 00:04:33,240
+replication فبالتالي هتأثر على نمو الميكروب زي ما
+
+58
+00:04:33,240 --> 00:04:36,820
+احنا بنلاحظ فيهالصورة هان القواعد النيتروجينية
+
+59
+00:04:36,820 --> 00:04:41,120
+الـA والـT فاندي هان هيه رابطة ما بين الـA والـT
+
+60
+00:04:41,120 --> 00:04:46,520
+لكن لما انا عرضتها هذا طبعا normal of DNA لكن لما
+
+61
+00:04:46,520 --> 00:04:50,700
+انا عرضتها لـUV صار عندي اتكون الـthiamine ديامر
+
+62
+00:04:50,700 --> 00:04:54,460
+اللي هي فككت الرابطة زي ما احنا ملاحظين بين الـE
+
+63
+00:04:54,460 --> 00:04:58,780
+والـT كونت رابطة ما بين الـT والـT وخلتهم مع بعض
+
+64
+00:04:58,780 --> 00:05:03,920
+هيك الـDNA اتعطل فبالتالي اتعطل الـreplication
+
+65
+00:05:08,810 --> 00:05:12,930
+في هاي الـ slide نتكلم على مصطلحات مهمة أول مصطلح
+
+66
+00:05:12,930 --> 00:05:16,310
+لـ sterilization التعقيم هو مر معناه في أول
+
+67
+00:05:16,310 --> 00:05:21,570
+chapter في المساق وعرفناه أنه killing or removing
+
+68
+00:05:21,570 --> 00:05:26,370
+all forms of microbial life including endosperm هو
+
+69
+00:05:26,370 --> 00:05:31,890
+قتل كل ال microbial كل أشكال ال microbial life بما
+
+70
+00:05:31,890 --> 00:05:35,960
+فيهم ال endospermطبعا الـ Sterilization قلنا فيه
+
+71
+00:05:35,960 --> 00:05:38,740
+أن دي أكتر من طريقة للـ Sterilization منها الـ
+
+72
+00:05:38,740 --> 00:05:41,880
+Autoclave، الـ filtration، الـ hot oven، الـ
+
+73
+00:05:41,880 --> 00:05:45,800
+ethylene oxide بالنسبة للـ disinfection قلنا هي
+
+74
+00:05:45,800 --> 00:05:50,640
+عبارة عن كيميكال تستخدم للـ inhibition لتثبيت نمو
+
+75
+00:05:50,640 --> 00:05:54,460
+الكائن الحي لكن ال sterilization قلنا killing ال
+
+76
+00:05:54,460 --> 00:05:56,620
+disinfection inhibition the growth of
+
+77
+00:05:56,620 --> 00:06:00,160
+microorganisms لكن اللي بدنا نضيفه أن الـ
+
+78
+00:06:00,160 --> 00:06:04,280
+disinfection بتتكلم على الـ non-living surfaceإنه
+
+79
+00:06:04,280 --> 00:06:09,660
+أنا بستخدمها مواد كيميائية لجمادات زي الطاولة لكن
+
+80
+00:06:09,660 --> 00:06:14,120
+لو أنا بدي أحكي على الـ Living tissue بستخدم مصطلح
+
+81
+00:06:14,120 --> 00:06:18,040
+الـ Antiseptic our chemical used on living tissue
+
+82
+00:06:18,040 --> 00:06:20,680
+to inhibit the growth of microorganism يبقى الـ
+
+83
+00:06:20,680 --> 00:06:23,980
+disinfection والـ antiseptic بتشبهوا إنه بيستخدموا
+
+84
+00:06:23,980 --> 00:06:29,020
+للـinhibition لتثبيت نمو الكائنات الحية لكن
+
+85
+00:06:29,020 --> 00:06:33,190
+الإختلاف إنه disinfectionon non-living surface
+
+86
+00:06:33,190 --> 00:06:37,350
+للجمادات لكن لو أنا بستعمل مواد كيميائية على
+
+87
+00:06:37,350 --> 00:06:41,490
+الكائنات الحية بتكلم على الـ Antiseptic الـ
+
+88
+00:06:41,490 --> 00:06:45,230
+Chemotherapeutic Agent هو مصطلح شامل أي مادة
+
+89
+00:06:45,230 --> 00:06:49,470
+كيميائية تستخدم لقتل، destroy، kill or inhibit the
+
+90
+00:06:49,470 --> 00:06:52,630
+growth of microorganisms لكن بتكلم على الـ Living
+
+91
+00:06:52,630 --> 00:06:59,390
+tissueيبقى هي مصطلح عام تستخدم لأو للتعبير عن أي
+
+92
+00:06:59,390 --> 00:07:03,370
+مادة كيميائية تستخدم لقتل أو تثبيت الكائنات الحية
+
+93
+00:07:03,370 --> 00:07:10,870
+لكن أنا بتكلم على الـ Living tissue في
+
+94
+00:07:10,870 --> 00:07:14,270
+الـ slide الأقبل عرفنا الـ chemotherapeutic agent
+
+95
+00:07:14,270 --> 00:07:17,110
+اللي هي المواد الكيميائية اللي تستخدم لقتل أو
+
+96
+00:07:17,110 --> 00:07:22,430
+تثبيت الكائنات على in or on living tissue في أو في
+
+97
+00:07:22,430 --> 00:07:27,360
+الـ hostلكن لما أنا أتكلم على العملية اللي تستخدم
+
+98
+00:07:27,360 --> 00:07:31,160
+فيها المواد الكيميائية بحكي Antimicrobial
+
+99
+00:07:31,160 --> 00:07:34,520
+Chemotherapy يبقى الـ Antimicrobial Chemotherapy
+
+100
+00:07:34,520 --> 00:07:37,620
+هي العملية is the use of chemical هو استخدام
+
+101
+00:07:37,620 --> 00:07:41,780
+المواد الكيميائية to inhibit or kill microorganism
+
+102
+00:07:41,780 --> 00:07:44,960
+in or on the host الhost يعني الـ living tissue
+
+103
+00:07:44,960 --> 00:07:49,220
+يبقى هذا المصطلح والمصطلح اللي ذكرناه أخرش الـ
+
+104
+00:07:49,220 --> 00:07:52,800
+Chemotherapeutic agentهم تقريبًا نفس المصطلح لكن
+
+105
+00:07:52,800 --> 00:07:55,680
+الـ Chemotherapeutic Agent عبارة عن كيميكا اللي هي
+
+106
+00:07:55,680 --> 00:07:59,160
+المواد الكيميائية نفسها لكن الـ Antimicrobial
+
+107
+00:07:59,160 --> 00:08:02,300
+Chemotherapy أنا بتكلم عن العملية اللي أنا بستخدم
+
+108
+00:08:02,300 --> 00:08:07,560
+فيها المواد الكيميائية لتثبيت أو قتل ال micro
+
+109
+00:08:07,560 --> 00:08:12,320
+organism in or on the host or living tissue طيب لو
+
+110
+00:08:12,320 --> 00:08:16,880
+أنا بدي أقسم الـ Antimicrobial Chemotherapeutic
+
+111
+00:08:16,880 --> 00:08:23,810
+Agent بنقسمهم لصنفينأول صنف اللي هو الـ Antibiotic
+
+112
+00:08:23,810 --> 00:08:27,430
+الـ Antibiotic substance produced as metabolic
+
+113
+00:08:27,430 --> 00:08:32,090
+product of one microorganism which inhibit or kill
+
+114
+00:08:32,090 --> 00:08:38,310
+other microorganism هي تأخذ من كائن حي حتى يؤثر
+
+115
+00:08:38,310 --> 00:08:43,210
+على كائن حي آخر باخدها من العمليات الأيضية حتى
+
+116
+00:08:43,210 --> 00:08:48,230
+يؤثر عليها من خلال التصبيت أو القتل على ال living
+
+117
+00:08:48,230 --> 00:08:53,200
+tissue زي ما ذكرنالكن بالنسبة لمصطلح التاني الـ
+
+118
+00:08:53,200 --> 00:08:56,540
+Antimicrobial Chemotherapeutic Chemical Chemical
+
+119
+00:08:56,540 --> 00:09:01,860
+هي كيميكالز بيتم إنتاجها في المختبر in laboratory
+
+120
+00:09:01,860 --> 00:09:06,580
+المهان بيتم إنتاجها أو بيتم استخلاصها من كائنات
+
+121
+00:09:06,580 --> 00:09:10,000
+حية لكن الـ Antimicrobial Chemotherapeutic
+
+122
+00:09:10,000 --> 00:09:14,520
+Chemical هي مواد كيميائية بيتم تصنيعهافي المختبرات
+
+123
+00:09:14,520 --> 00:09:19,800
+حتى تأثر على الـ microorganism إما بالتفبيت أو في
+
+124
+00:09:19,800 --> 00:09:26,080
+القطن هذا بالنسبة للفرق بينهم بالنسبة للـ
+
+125
+00:09:26,080 --> 00:09:29,420
+Antimicrobial chemotherapy هي العملية لكن لما
+
+126
+00:09:29,420 --> 00:09:34,020
+بنتكلم على ال chemical نفسها بنقول ال chemotherapy
+
+127
+00:09:34,020 --> 00:09:39,680
+agentالـ Chemotherapeutic Agents بيصنفوا لصنفين
+
+128
+00:09:39,680 --> 00:09:42,540
+اللي هم الـ Antibiotic وAntimicrobial
+
+129
+00:09:42,540 --> 00:09:46,100
+Chemotherapeutic Chemicals الـ Antibiotic هي مواد
+
+130
+00:09:46,100 --> 00:09:50,440
+من كائنات حية تستخلص من كائن حي لكن الـ
+
+131
+00:09:50,440 --> 00:09:55,000
+Antimicrobial Chemotherapeutic Chemicals يتم
+
+132
+00:09:55,000 --> 00:09:56,760
+تصنيعها في المختبر
+
+133
+00:10:00,140 --> 00:10:03,300
+في الـ slide اللي قبل صنفنا الـ Antimicrobial
+
+134
+00:10:03,300 --> 00:10:07,720
+Agent حسب الـ origin حسب المصدر لـ Antibiotic و
+
+135
+00:10:07,720 --> 00:10:11,040
+Antimicrobial Chemotherapeutic Chemical لأنه قلنا
+
+136
+00:10:11,040 --> 00:10:14,740
+أن الـ Antibiotic تستخلص من كائن حي لكن الـ
+
+137
+00:10:14,740 --> 00:10:18,560
+Antimicrobial Chemotherapeutic Chemical كانت من
+
+138
+00:10:18,560 --> 00:10:22,680
+المختبر يتم تصنيحها في المختبر هلأ بنصنف الـ
+
+139
+00:10:22,680 --> 00:10:27,120
+Antimicrobial Agent حسب الـ action حسب الـ action
+
+140
+00:10:27,120 --> 00:10:32,750
+تصنف إلى اتنينوstatic شو يعني sideal؟ sideal اللي
+
+141
+00:10:32,750 --> 00:10:36,590
+هو the kill microorganism اللي بتعمل قتل لل micro
+
+142
+00:10:36,590 --> 00:10:40,090
+organism لأن احنا اتفقنا ان ال antimicrobial agent
+
+143
+00:10:40,090 --> 00:10:45,230
+مصطلح عام يستخدم لقتل او تفضيل فلو كان ال action
+
+144
+00:10:45,230 --> 00:10:49,510
+sideal يبقى أنا بتكلم على ال kill microorganism في
+
+145
+00:10:49,510 --> 00:10:54,210
+عندي أمثلة على antibiotic تستخدم في قتل ال micro
+
+146
+00:10:54,210 --> 00:10:58,550
+organism بنسلين، سفالوسبورين، ستربتميسين،
+
+147
+00:10:58,550 --> 00:11:03,790
+نيموميسينهذا بالنسبة لـ Stidal بالنسبة للـ Static
+
+148
+00:11:03,790 --> 00:11:07,430
+اللي هي Inhibit microbial growth long enough for
+
+149
+00:11:07,430 --> 00:11:10,830
+the body's own defense to remove the organism
+
+150
+00:11:10,830 --> 00:11:16,310
+بتعمل على تثبيت نمو الكائن الحي من الأمثل على الـ
+
+151
+00:11:16,310 --> 00:11:20,350
+Antibiotic أو الـ Antimicrobial agent اللي static
+
+152
+00:11:20,350 --> 00:11:25,950
+in action Tetracycline erythromycin sulfonamide
+
+153
+00:11:26,090 --> 00:11:30,270
+هدول كلهم Antimicrobial Agent بالنسبة لـ Action
+
+154
+00:11:30,270 --> 00:11:34,070
+Static بتعمل Inhibition للميكروبيا لجراسه لكن
+
+155
+00:11:34,070 --> 00:11:37,810
+بالنسبة لـ Antimicrobial Agent اللي بتعمل قتل
+
+156
+00:11:37,810 --> 00:11:41,330
+Cydal in Action بنسلن، Cephalosporin،
+
+157
+00:11:41,330 --> 00:11:45,890
+Streptomycin، Neomycin هدول كلهم حفظ مطلوب انكوا
+
+158
+00:11:45,890 --> 00:11:50,130
+حفظ شهو الـ Antimicrobial Agent اللي بتعمل Cydal
+
+159
+00:11:50,130 --> 00:11:53,840
+in Action ومين اللي بتعمل Static in Actionطب نيجي
+
+160
+00:11:53,840 --> 00:11:58,140
+لمصطلح مهم اللي هو selective toxicity selective
+
+161
+00:11:58,140 --> 00:12:02,720
+toxicity شوها ده المصطلح معناه السمية الإختيارية
+
+162
+00:12:02,720 --> 00:12:07,360
+بيسميها that the agent used must inhibit or kill
+
+163
+00:12:07,360 --> 00:12:11,440
+the microorganism without seriously harming the
+
+164
+00:12:11,440 --> 00:12:16,480
+host بقوللي إن ال chemicals أو الـ antimicrobial
+
+165
+00:12:16,480 --> 00:12:19,380
+agent اللي بيعمل inhibition or kill ال micro
+
+166
+00:12:19,380 --> 00:12:24,170
+organismلازم أنه يعمل هذا الـ Action يثبت أو يقتل
+
+167
+00:12:24,170 --> 00:12:28,290
+الميكروب من غير ما يأثر على الـ Host على العائل
+
+168
+00:12:28,290 --> 00:12:33,290
+بأي أدرار هذا بالنسبة لـ Selective Toxicity أنه
+
+169
+00:12:33,290 --> 00:12:37,670
+الـ Antimicrobial Agent لازم يثبت أو يقتل الميكروب
+
+170
+00:12:37,670 --> 00:12:43,310
+من غير ما يأثر على العائل بأي أدرار طبيعي طبعا أنه
+
+171
+00:12:43,310 --> 00:12:47,690
+يكون فيه side effect أثر جانبية لكن لازم أنها تكون
+
+172
+00:12:47,690 --> 00:12:51,630
+غير مؤذية للعائلهذا بالنسبة لتصنيف الـ
+
+173
+00:12:51,630 --> 00:12:58,490
+Antimicrobial Agent حسب الـ Action قلنا
+
+174
+00:12:58,490 --> 00:13:02,190
+إن الـ Antimicrobial Agent بصنفه حسب أول شيء الـ
+
+175
+00:13:02,190 --> 00:13:05,930
+Origin لـ Antibiotic و Antimicrobial كيموثيربيوتك
+
+176
+00:13:05,930 --> 00:13:10,710
+كيميكال حسب الـ Action لـ Cydal و Static حسب، هلأ
+
+177
+00:13:10,710 --> 00:13:14,950
+نيجي لـ Spectrum حسب المادة أو المجال اللي بتأثر
+
+178
+00:13:14,950 --> 00:13:19,320
+عليههي الـ Antimicrobial agent من صنفه لـ Broad
+
+179
+00:13:19,320 --> 00:13:23,580
+Spectrum و ل Narrow Spectrum بالنسبة لـ Broad
+
+180
+00:13:23,580 --> 00:13:28,680
+Spectrum اللي هو واسع الطيف Drugs which are
+
+181
+00:13:28,680 --> 00:13:32,080
+effective agents of a variety of both Gram
+
+182
+00:13:32,080 --> 00:13:35,300
+-positive and Gram-negative bacteria من اسمه واسع
+
+183
+00:13:35,300 --> 00:13:38,900
+الطيف فبالتالي بيأثر على كل من ال Gram-positive
+
+184
+00:13:38,900 --> 00:13:42,640
+and Gram-negative bacteriaمن الأمثلة على الـ
+
+185
+00:13:42,640 --> 00:13:46,060
+Antimicrobial Agents اللي هي الـ Broad Spectrum
+
+186
+00:13:46,060 --> 00:13:50,860
+وهدول حفظ تتراسيكلينستربتومايسين سيفالوسبورين
+
+187
+00:13:50,860 --> 00:13:56,120
+أمبيسيلين سالفون أماي هدول أمثلة على Antimicrobial
+
+188
+00:13:56,120 --> 00:14:00,000
+Agents تعتبر Broad Spectrum طيب، بالنسبة للـ
+
+189
+00:14:00,000 --> 00:14:03,020
+Narrow Spectrum طبعا قلنا تصنيفان Antimicrobial
+
+190
+00:14:03,020 --> 00:14:06,160
+Agents حسب الـ Spectrum لـ Broad و Narrow عرفنا شو
+
+191
+00:14:06,160 --> 00:14:12,610
+يعني Broad Narrow من معناها ضيقهيأثر على نوع معين
+
+192
+00:14:12,610 --> 00:14:16,010
+من البكتيريا على مجموعة معينة drug which are
+
+193
+00:14:16,010 --> 00:14:20,130
+effective against just gram positive bacteria or
+
+194
+00:14:20,130 --> 00:14:23,790
+just gram negative bacteria or only a few species
+
+195
+00:14:23,790 --> 00:14:27,810
+يبقى هي بتأثر فقط على ال gram negative أو ممكن
+
+196
+00:14:27,810 --> 00:14:30,430
+أنها تأثر فقط على ال gram positive أو ممكن أنها
+
+197
+00:14:30,430 --> 00:14:35,410
+تأثر فقط على a few speciesمن الأمثلة على البكتيريا
+
+198
+00:14:35,410 --> 00:14:38,530
+اللى أو من الأمثلة على الـ Antimicrobial agent
+
+199
+00:14:38,530 --> 00:14:42,330
+اللى تعتبر narrow spectrum اللى هى الـ penicillin
+
+200
+00:14:42,330 --> 00:14:46,190
+الاريثروميسين الـ glendamycin والجنتاميسين هدول
+
+201
+00:14:46,190 --> 00:14:50,830
+كلهم يعتبروا narrow spectrum طيب مين أفضل ال
+
+202
+00:14:50,830 --> 00:14:54,450
+narrow spectrum او ال broad spectrum بقوللي ان ال
+
+203
+00:14:54,450 --> 00:14:59,170
+narrow spectrum هو الأفضل ليش هو الأفضل أول سبب
+
+204
+00:14:59,170 --> 00:15:03,760
+اللى هو مش مذكورلأنه more specific مختص بشكل أكتر
+
+205
+00:15:03,760 --> 00:15:08,460
+لأنه بيستهدف نوع معين هذا بالنسبة لأول سبب السبب
+
+206
+00:15:08,460 --> 00:15:12,740
+التاني بيقوللي انه الـ Neurospectrum it will cause
+
+207
+00:15:12,740 --> 00:15:16,760
+less distraction to the body to the body's normal
+
+208
+00:15:16,760 --> 00:15:21,260
+efflora قبل ما نذكر أو نحكي عن هذا السبب خلينا
+
+209
+00:15:21,260 --> 00:15:26,340
+نعرف شو هي ال normal efflora ال normal efflora
+
+210
+00:15:26,340 --> 00:15:32,160
+بتكون بكتيريا مفيدةعلى سبيل المثال مثلا في ال ..
+
+211
+00:15:32,160 --> 00:15:35,680
+ال .. ال .. في أي مكان في الجسم بيكون فيه عندي
+
+212
+00:15:35,680 --> 00:15:40,240
+بكتيريا مفيدة بيكون عاملة زي خط دفع لو .. لو أنا
+
+213
+00:15:40,240 --> 00:15:44,640
+أعطيت المريض Broad Spectrum يعني Antimicrobial
+
+214
+00:15:44,640 --> 00:15:48,320
+Agent بيكون واسع التأثير بيأثر على الـ Gram
+
+215
+00:15:48,320 --> 00:15:52,340
+Positive والـ Gram Negative بكتيريا إيش هيصير؟ رح
+
+216
+00:15:52,340 --> 00:15:56,240
+يقتل هذا الـ Antimicrobial Agent رح يقتل أو يثبت
+
+217
+00:15:56,240 --> 00:16:02,070
+حسب اللي هو شنوهرح يعمل على قتل أو تثبيت الميكروب
+
+218
+00:16:02,070 --> 00:16:06,590
+والـ Normal flora كمان فبالتالي راح تروح البكتيريا
+
+219
+00:16:06,590 --> 00:16:11,490
+الانتهازية الـ abortionistic microorganism تعمل
+
+220
+00:16:11,490 --> 00:16:16,090
+super infection طبعا، طيب، هلأ لو أنا استخدمت
+
+221
+00:16:16,090 --> 00:16:19,370
+narrow spectrum هذا الاشي مش هيصير فبالتالي will
+
+222
+00:16:19,370 --> 00:16:22,750
+cause less destruction to the body's normal flora
+
+223
+00:16:23,520 --> 00:16:27,840
+هيعمل إنه هيكون أقل ضرر لبكتيريا الـ normal flora
+
+224
+00:16:27,840 --> 00:16:32,860
+هاي بالتالي هيحافظ عليها هتظلها تعمل الوظائف
+
+225
+00:16:32,860 --> 00:16:37,560
+المختلفة لإلها طيب، هنكتب لإن سوء استخدام أو
+
+226
+00:16:37,560 --> 00:16:41,200
+استخدام للـ broad-spectrum antibiotic can lead to
+
+227
+00:16:41,200 --> 00:16:43,840
+super infection بيقدي لsuper infection by
+
+228
+00:16:43,840 --> 00:16:46,740
+abortionistic microorganism شو يعني super
+
+229
+00:16:46,740 --> 00:16:50,400
+infection يعني؟نتيجة خلل معين على سبيل المثال
+
+230
+00:16:50,400 --> 00:16:54,500
+استخدام Antibiotic R Antimicrobial Agent Broad
+
+231
+00:16:54,500 --> 00:16:58,780
+Spectrum راح تقضي على الـNormaliflora وعلى
+
+232
+00:16:58,780 --> 00:17:02,640
+الميكروب راح تيجي البكتيريا الانتهازية وتسبب لإله
+
+233
+00:17:02,640 --> 00:17:06,300
+عدوى هذه العدوى بسميها Super Infection لأن هو
+
+234
+00:17:06,300 --> 00:17:11,180
+بالأساس ما كان له عدوى لكن نتيجة اللي هو استخدام
+
+235
+00:17:11,180 --> 00:17:15,120
+الـBroad Spectrum Antimicrobial Agent صار هذا
+
+236
+00:17:15,120 --> 00:17:18,970
+الأشياءلكن لو أنا بتكلم على الـ Primary Infection
+
+237
+00:17:18,970 --> 00:17:23,990
+بتكون العدوى ابتجيني من طبعا من بكتيريا ممرضة
+
+238
+00:17:23,990 --> 00:17:28,810
+وبتسبب المرض هذا بالنسبة لآخر تصنيف من تصنيفات الـ
+
+239
+00:17:28,810 --> 00:17:32,610
+Antimicrobial Agent حسب الـ Spectrum ومطلوب حفظ
+
+240
+00:17:32,610 --> 00:17:35,530
+اللي هو الأمثلة على الـ Broad Spectrum و Narrow
+
+241
+00:17:35,530 --> 00:17:39,750
+Spectrum واخر شئ لازم نعرف ليش أنا أو شو بفضل أكيد
+
+242
+00:17:39,750 --> 00:17:42,990
+بنفضل الـ Narrow Spectrum لأنه more specific تاني
+
+243
+00:17:42,990 --> 00:17:46,870
+الشيء لأنهwill cause less destruction to the
+
+244
+00:17:46,870 --> 00:17:50,830
+body's normal flora لو أنا استخدمت الـ Broad
+
+245
+00:17:50,830 --> 00:17:53,990
+Spectrum Antimicrobial Agent هيسببلي super
+
+246
+00:17:53,990 --> 00:17:58,150
+infection by abortionistic microorganism وعرفنا شو
+
+247
+00:17:58,150 --> 00:18:01,710
+يعني super infection وشو يعني primary infection
+
+248
+00:18:05,420 --> 00:18:09,280
+في هاي الـ slide هنتكلم على مصطلحات مهمة هو
+
+249
+00:18:09,280 --> 00:18:13,280
+المصطلح الـ antibiotic-resistant اللي هي مقاومة
+
+250
+00:18:13,280 --> 00:18:18,160
+المضادات الحيوية bacteria gain ability to grow no
+
+251
+00:18:18,160 --> 00:18:22,760
+longer sensitive to a drug إنه البكتيريا بيصير
+
+252
+00:18:22,760 --> 00:18:27,460
+عندها القدرة إنها تنمو حتى بوجود المضاد الحيوي
+
+253
+00:18:27,460 --> 00:18:31,860
+بطلت تتأثر في الـ antimicrobial agent
+
+254
+00:18:33,080 --> 00:18:37,400
+Antiretroviral هي عبارة عن مضادات بتشتغل ضد
+
+255
+00:18:37,400 --> 00:18:41,560
+الفيروسات طب نيجي ل ال combination of a drug اللي
+
+256
+00:18:41,560 --> 00:18:45,260
+هو اتحاد الأدوية فيه عندي تلت مصطلحات مهمة بالنسبة
+
+257
+00:18:45,260 --> 00:18:49,960
+ل ال combination of a drug synergism، antagonism و
+
+258
+00:18:49,960 --> 00:18:54,820
+additive بالنسبة لأول واحد اللي هو synergism
+
+259
+00:18:54,820 --> 00:18:58,860
+synergism يعني action of two antibiotic greater
+
+260
+00:18:58,860 --> 00:19:04,630
+مجموع تأثيرفعالية المضادات الحيوية لإتنين أكبر من
+
+261
+00:19:04,630 --> 00:19:10,070
+تأثير كل مضاد حيوي على حدا يعني واحد زائد واحد
+
+262
+00:19:10,070 --> 00:19:13,350
+بيكون مش بيساوي اتنين أكبر من اتنين لأن مجموع
+
+263
+00:19:13,350 --> 00:19:17,810
+تأثير فعليتهم مع بعض أكبر من تأثير كل مضاد حيوي
+
+264
+00:19:17,810 --> 00:19:22,650
+على حدا فبالتالي في هاي الحالة ميح انه ناخد هدول
+
+265
+00:19:22,650 --> 00:19:27,230
+الأدوية مع بعض لأن تأثيرهم مع بعض أكبر من تأثير كل
+
+266
+00:19:27,230 --> 00:19:31,980
+مضاد حيوي على حدابالنسبة لـ Antagonism اللي هو
+
+267
+00:19:31,980 --> 00:19:36,080
+action of drug is reduced less effective إنه مجموع
+
+268
+00:19:36,080 --> 00:19:40,060
+تأثير الـ two drug مع بعض أقل من تأثير كل واحد على
+
+269
+00:19:40,060 --> 00:19:45,220
+حدا يعني واحد زائد واحد بيكون أقل من اتنين أخر
+
+270
+00:19:45,220 --> 00:19:49,300
+شائل هي ال additive the action of two drugs in
+
+271
+00:19:49,300 --> 00:19:52,140
+combination is equal to the sum of their
+
+272
+00:19:52,140 --> 00:19:55,540
+independent activity when studied separatelyبقول
+
+273
+00:19:55,540 --> 00:19:59,880
+لي إن مجموع تأثير المضادين الحيويين بيساوي تأثير
+
+274
+00:19:59,880 --> 00:20:03,680
+كل مضاد حيوي على حد يعني واحد زائد واحد بيساوي
+
+275
+00:20:03,680 --> 00:20:08,220
+اتنين هذا بالنسبة لـ Combination of a Drug نيجي
+
+276
+00:20:08,220 --> 00:20:12,180
+لمصطلحين مهمين اللي هم الـ Minimum Inhibitory
+
+277
+00:20:12,180 --> 00:20:18,200
+Concentration MIC The lowest concentration of
+
+278
+00:20:18,200 --> 00:20:22,120
+chemotherapy agent capable of preventing growth of
+
+279
+00:20:22,120 --> 00:20:27,140
+the test organismهو أقل تركيز من الـ Antimicrobial
+
+280
+00:20:27,140 --> 00:20:31,740
+agent قادر على منع نمو البكتيريا شو منع نمو
+
+281
+00:20:31,740 --> 00:20:34,900
+البكتيريا، شو معناها، اللي هي تفضيط، Inhibition
+
+282
+00:20:34,900 --> 00:20:39,200
+عشان هيك أنا قولت Minimum inhibitory يعني هو أقل
+
+283
+00:20:39,200 --> 00:20:42,160
+تركيز من الـ Antimicrobial agent قادر إنه يعمل
+
+284
+00:20:42,160 --> 00:20:46,200
+Inhibition للبكتيريا Preventing the growth يعني
+
+285
+00:20:46,200 --> 00:20:50,640
+يعمل Inhibition لكن الـ Minimum bactericidal
+
+286
+00:20:50,640 --> 00:20:54,650
+concentrationاللي هو the lowest concentration of
+
+287
+00:20:54,650 --> 00:20:59,850
+chemotherapy agent ذات result is in no growth اللي
+
+288
+00:20:59,850 --> 00:21:05,230
+هي turbidity of subculture هو أقل تركيز من الـ
+
+289
+00:21:05,230 --> 00:21:09,830
+antibiotic أو الـ antimicrobial agent خاطر على قتل
+
+290
+00:21:09,830 --> 00:21:12,770
+البكتيريا، إنه يكون فيه عندي no growth no growth
+
+291
+00:21:12,770 --> 00:21:17,030
+يعني قتل البكتيريا، إن ما يكون فيه عندي بكتيريا في
+
+292
+00:21:17,030 --> 00:21:21,940
+الـ Lithiumأو ممكن نحكي في تعريف أدق اللي هو أقل
+
+293
+00:21:21,940 --> 00:21:26,280
+تركيز من الـ Antimicrobial agent بيسمح بنمو أقل من
+
+294
+00:21:26,280 --> 00:21:31,520
+1 من 10% من الميكروب هذا بالنسبة لـ Minimum
+
+295
+00:21:31,520 --> 00:21:34,720
+Inhibitory Concentration والـ Minimum Bacterioidal
+
+296
+00:21:34,720 --> 00:21:38,380
+Concentration الفصل الجاي في مساق الـ Diagnostic
+
+297
+00:21:38,380 --> 00:21:41,780
+Medical Microbiology هتتعرفوا بالتفصيل كيف أنا بدي
+
+298
+00:21:41,780 --> 00:21:46,020
+أحسب الـ Minimum Inhibitory Concentration والـ
+
+299
+00:21:46,020 --> 00:21:48,300
+Minimum Bacterioidal Concentration
+
+300
+00:21:53,540 --> 00:21:58,120
+Antimicrobial Activity كيف تأثير أو كيف بيأثر الـ
+
+301
+00:21:58,120 --> 00:22:02,580
+Antimicrobial Agent على الخلية في عندي خمس طرق of
+
+302
+00:22:02,580 --> 00:22:08,680
+Antimicrobial Activity هنذكرهم بشكل مختصر الفصل
+
+303
+00:22:08,680 --> 00:22:11,520
+الجاي في مادة الـ Diagnostic Medical Microbiology
+
+304
+00:22:11,520 --> 00:22:16,300
+هيكون فيه chapter كامل على هذا الموضوع يعني هيكون
+
+305
+00:22:16,300 --> 00:22:21,530
+فيه تفاصيل أكتر لكن في هذا الفصل فقط هنذكربعض
+
+306
+00:22:21,530 --> 00:22:26,450
+المعلومات الأساسية بالنسبة لأول طريقة Injury to
+
+307
+00:22:26,450 --> 00:22:29,490
+plasma membrane ممكن أنه الـ Antimicrobial agent
+
+308
+00:22:29,490 --> 00:22:32,050
+يأثر على الـ plasma membrane مثال على
+
+309
+00:22:32,050 --> 00:22:36,330
+Antimicrobial agent الـ Polymyxin B هو هذا بيأثر
+
+310
+00:22:36,330 --> 00:22:39,730
+على ال plasma membrane ممكن أنه يعمل Inhibition of
+
+311
+00:22:39,730 --> 00:22:43,850
+cell wall synthesisمثال عليا الـ Penicillin و الـ
+
+312
+00:22:43,850 --> 00:22:48,650
+Bacitracin وإحنا قولنا في أول معامل في المادة إن
+
+313
+00:22:48,650 --> 00:22:51,810
+الـ Penicillin بيعمل على تفكيك الرابط اللي كانت
+
+314
+00:22:51,810 --> 00:22:55,610
+بين الـBacterial glycan فبالتالي بيعمل Inhibition
+
+315
+00:22:55,610 --> 00:22:59,470
+للـ Cell Wall Synthesis ممكن يؤثر على الـ Protein
+
+316
+00:22:59,470 --> 00:23:03,950
+Synthesis بالتحديد اللي هي الـ Translation ممكن
+
+317
+00:23:03,950 --> 00:23:07,030
+يؤثر يعمل Inhibition of Nucleic Acid Replication
+
+318
+00:23:07,030 --> 00:23:10,930
+أو Retranscriptionممكن يعمل Inhibition of
+
+319
+00:23:10,930 --> 00:23:13,950
+Essential Metabolites هذا بالنسبة للـ
+
+320
+00:23:13,950 --> 00:23:19,290
+Antimicrobial Activity بالنسبة
+
+321
+00:23:19,290 --> 00:23:21,950
+للـ Antimicrobial Activity قولنا ممكن أنه يعمل
+
+322
+00:23:21,950 --> 00:23:25,870
+Injury للـ Plasma Membrane مثلًا عليه البولي
+
+323
+00:23:25,870 --> 00:23:30,410
+ميكسين بيه ممكن يعمل Inhibition للسل والـ
+
+324
+00:23:30,410 --> 00:23:32,990
+Synthesis قولنا في أمثلة الـ Penicillin،
+
+325
+00:23:32,990 --> 00:23:36,770
+الستفالوسبورين، البسترسين، الفانكمايسينممكن يعمل
+
+326
+00:23:36,770 --> 00:23:40,170
+Inhibition لـ Protein Synthesis مثال عليه بالأخص
+
+327
+00:23:40,170 --> 00:23:43,890
+اللي هو Translation لـ Chloramphenicol لـ
+
+328
+00:23:43,890 --> 00:23:47,470
+Erythromycin لـ Tetracycline لـ Streptomycin ممكن
+
+329
+00:23:47,470 --> 00:23:52,230
+يؤثر على اللي هو Inhibition of Synthesis of
+
+330
+00:23:52,230 --> 00:23:56,990
+Essential Metabolites ممكن يؤثر على اللي هو لـ
+
+331
+00:23:56,990 --> 00:24:00,450
+Nucleic Acid Replication and Transcription طبعا
+
+332
+00:24:00,450 --> 00:24:04,130
+احنا بس بناعرف اللي هو شو الـ Active شو الـ
+
+333
+00:24:04,130 --> 00:24:07,740
+Antimicrobial Activityالأمثلة في هاي الـ slide مش
+
+334
+00:24:07,740 --> 00:24:12,880
+مطلوب حفظ الفصل الجاي هتكون المادة في التفصيل لكن
+
+335
+00:24:12,880 --> 00:24:18,860
+احنا في هذا الفصل بس بدنا نعرف شو الطرق أو كيف الـ
+
+336
+00:24:18,860 --> 00:24:24,060
+Antimicrobial agent بيأثر على الخلية البكتيرية
+
+337
+00:24:24,060 --> 00:24:28,180
+مطلوب فقط حفظ اللي تم ذكره في ال slide اللي قبل
+
+338
+00:24:28,180 --> 00:24:33,030
+فقط أما في هاي ال slide فقط بدنا نعرف شوطرق بشكل
+
+339
+00:24:33,030 --> 00:24:37,290
+مختصر كيف بيأثر الـ Antimicrobial agent على الخلية
+
+340
+00:24:37,290 --> 00:24:41,850
+البكتيرية بالقتل أو بالتثبيت بدون حفظ الأمثلة لكن
+
+341
+00:24:41,850 --> 00:24:49,610
+مطلوب حفظ ما تم ذكره في ال slide اللي قبل How
+
+342
+00:24:49,610 --> 00:24:53,370
+to choose antimicrobial drug عوامل لازم أخدها بعين
+
+343
+00:24:53,370 --> 00:24:58,770
+الاعتبار قبل وصف أي antimicrobial drugهل هو Narrow
+
+344
+00:24:58,770 --> 00:25:02,970
+Spectrum أو Broad Spectrum قلنا يفضل إن أنا أستخدم
+
+345
+00:25:02,970 --> 00:25:06,190
+الـ Narrow Spectrum للأسباب اللي ذكرناها سابقة
+
+346
+00:25:06,190 --> 00:25:10,430
+ممكن إننا نستخدم الـ Broad Spectrum في حالات
+
+347
+00:25:10,430 --> 00:25:15,770
+إستثنائية هل المريض Old Age أو Child نعرف إنه الـ
+
+348
+00:25:15,770 --> 00:25:19,730
+Antibiotic فيه تركيز مختلفة فأكيد مش هعطي الـ
+
+349
+00:25:19,730 --> 00:25:23,810
+Child نفس تركيز الـ Old Age Male أو Female
+
+350
+00:25:23,810 --> 00:25:29,600
+Pregnant أو Lactating Womeninpatient or outpatient
+
+351
+00:25:29,600 --> 00:25:34,360
+inpatient اللي هو مريض داخل المشفة بيصرفوله برد
+
+352
+00:25:34,360 --> 00:25:38,680
+spectrum لأنه بيكون معرض للتعرض للكثير من
+
+353
+00:25:38,680 --> 00:25:44,480
+الميكروبات لكن ال outpatient اللي هو خارج المشفة
+
+354
+00:25:44,480 --> 00:25:49,200
+type of microorganism نوع ال micro organism site
+
+355
+00:25:49,200 --> 00:25:54,730
+of infection اللي هي مكان العدوىممكن تكون سطحية
+
+356
+00:25:54,730 --> 00:26:01,230
+فبيتم صرف مضاد حيوي على شكل برهم لكن لو كان داخل
+
+357
+00:26:01,230 --> 00:26:06,890
+الجسم بيتم إعطاءه capsule أو injection هذا بالنسبة
+
+358
+00:26:06,890 --> 00:26:10,970
+للعوامل اللي لازم أخدها بعين الاعتبار عند وصف أي
+
+359
+00:26:10,970 --> 00:26:18,650
+مضاد حيوي هاي
+
+360
+00:26:18,650 --> 00:26:22,840
+ال slide من chapter الـ Serial Dilutionحاب أنوه
+
+361
+00:26:22,840 --> 00:26:27,280
+لبعض المعلومات اللي ما ذكرتها في الـ Chapter هلأ
+
+362
+00:26:27,280 --> 00:26:31,080
+بالنسبة للـ Pour Eplate شو قصده في الـ Pour Eplate
+
+363
+00:26:31,080 --> 00:26:35,280
+للـ Pour Eplate إنه أنا بحط العينة قبل ما أنا أصب
+
+364
+00:26:35,280 --> 00:26:40,280
+الميديا هيك أنا بوفر ظروف لا هواقية لكن الـ Spread
+
+365
+00:26:40,280 --> 00:26:44,400
+Eplate الـ Spread Eplate بحط بتكون مصبوبة الميديا
+
+366
+00:26:44,400 --> 00:26:48,880
+بعدين بحط العينة هيك أنا بوفر ظروف هواقية طيب ليش
+
+367
+00:26:48,880 --> 00:26:50,660
+في ال slide ذاكرها
+
+368
+00:26:53,960 --> 00:26:58,420
+عشرة أساليب تلاتة وأخد من هان اللي هو من الـ
+
+369
+00:26:58,420 --> 00:27:01,780
+dilution عشرة أساليب تلاتة هان أخد من ال dilution
+
+370
+00:27:01,780 --> 00:27:06,000
+أو من الـ tube التخفيف تبعها عشرة أساليب اتنين
+
+371
+00:27:06,000 --> 00:27:10,180
+وذكر عشرة أساليب تلاتة أحنا قولنا في القانون الـ
+
+372
+00:27:10,180 --> 00:27:14,460
+concentration اللي هو الـ number of colony عدد ال
+
+373
+00:27:14,460 --> 00:27:20,040
+colony اللي موجودة على الطبق على الـ S اللي هي الـ
+
+374
+00:27:20,040 --> 00:27:24,110
+volume اللي أناأخدته من هايل يوتيوب وفردته أو
+
+375
+00:27:24,110 --> 00:27:29,990
+حطيته على البلاد ضرب الـ Dilution هذا الرقم هو
+
+376
+00:27:29,990 --> 00:27:35,590
+حاصل ضرب الـ Dilution مع الـ S الـ S ضرب اللي هو
+
+377
+00:27:35,590 --> 00:27:42,390
+الـ Dilution هذا الرقم هان أخدت واحد ملي من هايل
+
+378
+00:27:42,390 --> 00:27:45,710
+يوتيوب فانا ضربتها في تخفيفة اللي هي عشرة أسالب
+
+379
+00:27:45,710 --> 00:27:51,300
+تلاتة فطلع عشرة أسالب تلاتة لكن هانأخد واحد من
+
+380
+00:27:51,300 --> 00:27:58,000
+عشرة ML ضرب اللي هي العشرة أسالب اتنين فطلعت
+
+381
+00:27:58,000 --> 00:28:01,960
+العشرة أسالب تلاتة هذا بالنسبة للرقم ليش هان مكتوب
+
+382
+00:28:01,960 --> 00:28:06,140
+عشرة أسالب تلاتة هي الرقم هذا حاصل ضرب ال dilution
+
+383
+00:28:06,140 --> 00:28:11,160
+مع ال S هان أخدت واحد من عشرة لكن هان أخدت واحد ML
+
+384
+00:28:11,160 --> 00:28:17,960
+لازم يكون الرقم اللي هو ال S لازم يكون بال ML لو
+
+385
+00:28:17,960 --> 00:28:23,220
+كان في ال microلازم أحاوله لـ ML هذه معلومة مهمة
+
+386
+00:28:23,220 --> 00:28:30,400
+لازم ننتبه لإلها في المثاقات هيك
+
+387
+00:28:30,400 --> 00:28:35,680
+بنكون وصلنا لنهاية شرح المحاضرة وأيضا لنهاية سلسلة
+
+388
+00:28:35,680 --> 00:28:40,040
+محاضرات مساق الـ Essential Medical Microbiology
+
+389
+00:28:40,040 --> 00:28:45,180
+بتمنعكم كل التوفيق والنجاح والسلام عليكم ورحمة
+
+390
+00:28:45,180 --> 00:28:46,400
+الله وبركاته
+
diff --git a/PL9fwy3NUQKwZWgAb_V1ATlRRsH-6i3cPC/ssK-XRx65XI_raw.srt b/PL9fwy3NUQKwZWgAb_V1ATlRRsH-6i3cPC/ssK-XRx65XI_raw.srt
new file mode 100644
index 0000000000000000000000000000000000000000..36381d534d612d8ce88e652f74bb3a1c4c1fa732
--- /dev/null
+++ b/PL9fwy3NUQKwZWgAb_V1ATlRRsH-6i3cPC/ssK-XRx65XI_raw.srt
@@ -0,0 +1,1560 @@
+1
+00:00:01,620 --> 00:00:05,780
+فنبدأ بالجزء التاني من الـ Chapter اللي هو بتكلم
+
+2
+00:00:05,780 --> 00:00:09,820
+عن الـ Microbial Control Agent عوامل التحكم
+
+3
+00:00:09,820 --> 00:00:14,560
+بالميكروبات Many microorganisms are beneficial and
+
+4
+00:00:14,560 --> 00:00:18,880
+necessary for human wellbeing قولنا إن نسبة كبيرة
+
+5
+00:00:18,880 --> 00:00:23,540
+من الميكروبات تعتبر beneficial، مفيدة ونسبة قليلة
+
+6
+00:00:23,540 --> 00:00:28,160
+منها تعتبر non-pathogenic however, microbial
+
+7
+00:00:28,160 --> 00:00:30,740
+activities may have undesirable
+
+8
+00:00:38,540 --> 00:00:42,960
+بتسبب اللي هو تسامم الغذاء وتسبب العديد من الأمراض
+
+9
+00:00:42,960 --> 00:00:46,140
+عشان هيك it is essential to be able to kill
+
+10
+00:00:46,140 --> 00:00:50,420
+microorganism or inhibit their growth to minimize
+
+11
+00:00:51,860 --> 00:00:57,080
+عشان هيك لازم يكون هنا القدرة ان احنا نقتل ال
+
+12
+00:00:57,080 --> 00:01:00,960
+micro organism هاي او نعملي لها تثبيت لانمو عشان
+
+13
+00:01:00,960 --> 00:01:06,340
+نقلل هاي المخاطر بقوللي انه عندي اكتر من طريقة
+
+14
+00:01:06,340 --> 00:01:10,360
+للتخلص من الميكروبات في عندي ال physical method في
+
+15
+00:01:10,360 --> 00:01:14,380
+عندي ال chemical method و ال mechanical method
+
+16
+00:01:14,380 --> 00:01:19,520
+هيكلها طرق للتخلص من الميكروبات هنتكلم عن كل طريقة
+
+17
+00:01:19,850 --> 00:01:24,630
+وفي عندي في كل طريقة طبعا أنواع هنتكلم عنهم في ال
+
+18
+00:01:24,630 --> 00:01:29,530
+slides اللي جايه هنتكلم
+
+19
+00:01:29,530 --> 00:01:34,210
+على أول نوع من ال method of microbial control ال
+
+20
+00:01:34,210 --> 00:01:38,210
+physical method الطرق الفيزيائية ال radiation
+
+21
+00:01:38,210 --> 00:01:42,930
+الإشعاع هو مثال على ال physical method في عندي
+
+22
+00:01:42,930 --> 00:01:47,490
+أنواع من ال radiation من الإشعاعالـ Ionizing
+
+23
+00:01:47,490 --> 00:01:51,390
+Radiation والـ Non-Ionizing Radiation هنتكلم عنهم
+
+24
+00:01:51,390 --> 00:01:55,870
+بالتفصيل الـ
+
+25
+00:01:55,870 --> 00:01:59,510
+Ionizing Radiation deep penetration power that has
+
+26
+00:01:59,510 --> 00:02:03,610
+sufficient energy بقوللي أنه بتمتلك قوة اختراق
+
+27
+00:02:03,610 --> 00:02:08,490
+عالية نتيجة كمية الطاقة العالية الموجودة بالإشعار
+
+28
+00:02:08,490 --> 00:02:12,670
+هذا هيسبب to cause electron to leave their orbit
+
+29
+00:02:12,670 --> 00:02:17,820
+breaks DNAبالتالي راح يطلع عند الإلكترون من المدار
+
+30
+00:02:17,820 --> 00:02:23,300
+راح يخترق الحاجز ويكسر الـ DNA هك أنا بما أني أنا
+
+31
+00:02:23,300 --> 00:02:27,920
+كسرت الـ DNA تم تعطيل عملية الـ replication التطاع
+
+32
+00:02:27,920 --> 00:02:33,060
+في الخلية فبالتالي هك هيأثر على نمو الميكروب من
+
+33
+00:02:33,060 --> 00:02:36,960
+الأمثلة على الـ Ionizing Radiation الـ Gamma-Rays
+
+34
+00:02:36,960 --> 00:02:40,920
+الـ X-Rays والـ Cathode-Rays هذه الطريقة تستخدم
+
+35
+00:02:49,820 --> 00:02:53,620
+النوع التاني من الرادياشن الـ Non-Ionizing
+
+36
+00:02:53,620 --> 00:02:58,680
+Radiation هفليتل بنترياشن بوار بتمتلك قوة اختراق
+
+37
+00:02:58,680 --> 00:03:02,360
+قليلة لأن الطاقة اللي بتمتلكها أقل على عكس ال
+
+38
+00:03:02,360 --> 00:03:07,170
+Ionizing اللي كانت بتمتلك قوة اختراق عاليةand must
+
+39
+00:03:07,170 --> 00:03:11,090
+be directly exposed بقولي لازم بشكل مباشر تتعرض
+
+40
+00:03:11,090 --> 00:03:15,070
+لالميكروب في ال ionizing radiation كان فيه أندي
+
+41
+00:03:15,070 --> 00:03:20,430
+إلكترون هو اللي يتعرض لالميكروب لكن هان بشكل مباشر
+
+42
+00:03:20,430 --> 00:03:25,350
+هي نفسها ال non-ionizing radiation هي اللي هتتعرض
+
+43
+00:03:25,350 --> 00:03:29,690
+لالميكروب بشكل مباشر ما بيخرج منها ايونات نطال
+
+44
+00:03:29,690 --> 00:03:34,070
+عليها ال UV light ال UV light بقولي creates تايمان
+
+45
+00:03:34,070 --> 00:03:38,140
+ديامر أش يعني تايمان ديامرهلأ لما أنا أعرض
+
+46
+00:03:38,140 --> 00:03:43,240
+البكتيريا لـ UV light البكتيريا فيها الـ DNA
+
+47
+00:03:43,240 --> 00:03:48,780
+المادة الوراثية هلأ المادة الوراثية طبعا هي سلسلة
+
+48
+00:03:48,780 --> 00:03:52,560
+من القواعد النيتروجينية فيه بيكون عندي القاعد
+
+49
+00:03:52,560 --> 00:03:56,240
+النيتروجينية الـ A والـ T بتكون رابط ما بينهم
+
+50
+00:03:56,240 --> 00:04:01,160
+بتكون رابط ما بين الـ A والـ T هلأ الـ UV light لو
+
+51
+00:04:01,160 --> 00:04:07,460
+أنا عرضتها للبكتيريا يعني للـ DNAرح تفكك الرابطة
+
+52
+00:04:07,460 --> 00:04:11,700
+ما بين الـ A و الـ T هتفكك الرابطة ما بين الـ A و
+
+53
+00:04:11,700 --> 00:04:16,960
+الـ T و هتخلي الرابطة ما بين الـ T و الـ T و رح
+
+54
+00:04:16,960 --> 00:04:20,040
+يرتبطوا و يكونوا الثيامان ديامر هذا بالنسبة
+
+55
+00:04:20,040 --> 00:04:24,580
+لثيامان ديامر which interfere with replication
+
+56
+00:04:24,580 --> 00:04:28,520
+طبعا بالتالي هي عطلت الـ DNA عطلت عملية الـ
+
+57
+00:04:28,520 --> 00:04:33,240
+replication فبالتالي هتأثر على نمو الميكروب زي ما
+
+58
+00:04:33,240 --> 00:04:36,820
+احنا بنلاحظ فيهالصورة هان القواعد النيتروجينية
+
+59
+00:04:36,820 --> 00:04:41,120
+الـA والـT فاندي هان هيه رابطة ما بين الـA والـT
+
+60
+00:04:41,120 --> 00:04:46,520
+لكن لما انا عرضتها هذا طبعا normal of DNA لكن لما
+
+61
+00:04:46,520 --> 00:04:50,700
+انا عرضتها لـUV صار عندي اتكون الـthiamine ديامر
+
+62
+00:04:50,700 --> 00:04:54,460
+اللي هي فككت الرابطة زي ما احنا ملاحظين بين الـE
+
+63
+00:04:54,460 --> 00:04:58,780
+والـT كونت رابطة ما بين الـT والـT وخلتهم مع بعض
+
+64
+00:04:58,780 --> 00:05:03,920
+هيك الـDNA اتعطل فبالتالي اتعطل الـreplication
+
+65
+00:05:08,810 --> 00:05:12,930
+في هاي الـ slide نتكلم على مصطلحات مهمة أول مصطلح
+
+66
+00:05:12,930 --> 00:05:16,310
+لـ sterilization التعقيم هو مر معناه في أول
+
+67
+00:05:16,310 --> 00:05:21,570
+chapter في المساق وعرفناه أنه killing or removing
+
+68
+00:05:21,570 --> 00:05:26,370
+all forms of microbial life including endosperm هو
+
+69
+00:05:26,370 --> 00:05:31,890
+قتل كل ال microbial كل أشكال ال microbial life بما
+
+70
+00:05:31,890 --> 00:05:35,960
+فيهم ال endospermطبعا الـ Sterilization قلنا فيه
+
+71
+00:05:35,960 --> 00:05:38,740
+أن دي أكتر من طريقة للـ Sterilization منها الـ
+
+72
+00:05:38,740 --> 00:05:41,880
+Autoclave، الـ filtration، الـ hot oven، الـ
+
+73
+00:05:41,880 --> 00:05:45,800
+ethylene oxide بالنسبة للـ disinfection قلنا هي
+
+74
+00:05:45,800 --> 00:05:50,640
+عبارة عن كيميكال تستخدم للـ inhibition لتثبيت نمو
+
+75
+00:05:50,640 --> 00:05:54,460
+الكائن الحي لكن ال sterilization قلنا killing ال
+
+76
+00:05:54,460 --> 00:05:56,620
+disinfection inhibition the growth of
+
+77
+00:05:56,620 --> 00:06:00,160
+microorganisms لكن اللي بدنا نضيفه أن الـ
+
+78
+00:06:00,160 --> 00:06:04,280
+disinfection بتتكلم على الـ non-living surfaceإنه
+
+79
+00:06:04,280 --> 00:06:09,660
+أنا بستخدمها مواد كيميائية لجمادات زي الطاولة لكن
+
+80
+00:06:09,660 --> 00:06:14,120
+لو أنا بدي أحكي على الـ Living tissue بستخدم مصطلح
+
+81
+00:06:14,120 --> 00:06:18,040
+الـ Antiseptic our chemical used on living tissue
+
+82
+00:06:18,040 --> 00:06:20,680
+to inhibit the growth of microorganism يبقى الـ
+
+83
+00:06:20,680 --> 00:06:23,980
+disinfection والـ antiseptic بتشبهوا إنه بيستخدموا
+
+84
+00:06:23,980 --> 00:06:29,020
+للـinhibition لتثبيت نمو الكائنات الحية لكن
+
+85
+00:06:29,020 --> 00:06:33,190
+الإختلاف إنه disinfectionon non-living surface
+
+86
+00:06:33,190 --> 00:06:37,350
+للجمادات لكن لو أنا بستعمل مواد كيميائية على
+
+87
+00:06:37,350 --> 00:06:41,490
+الكائنات الحية بتكلم على الـ Antiseptic الـ
+
+88
+00:06:41,490 --> 00:06:45,230
+Chemotherapeutic Agent هو مصطلح شامل أي مادة
+
+89
+00:06:45,230 --> 00:06:49,470
+كيميائية تستخدم لقتل، destroy، kill or inhibit the
+
+90
+00:06:49,470 --> 00:06:52,630
+growth of microorganisms لكن بتكلم على الـ Living
+
+91
+00:06:52,630 --> 00:06:59,390
+tissueيبقى هي مصطلح عام تستخدم لأو للتعبير عن أي
+
+92
+00:06:59,390 --> 00:07:03,370
+مادة كيميائية تستخدم لقتل أو تثبيت الكائنات الحية
+
+93
+00:07:03,370 --> 00:07:10,870
+لكن أنا بتكلم على الـ Living tissue في
+
+94
+00:07:10,870 --> 00:07:14,270
+الـ slide الأقبل عرفنا الـ chemotherapeutic agent
+
+95
+00:07:14,270 --> 00:07:17,110
+اللي هي المواد الكيميائية اللي تستخدم لقتل أو
+
+96
+00:07:17,110 --> 00:07:22,430
+تثبيت الكائنات على in or on living tissue في أو في
+
+97
+00:07:22,430 --> 00:07:27,360
+الـ hostلكن لما أنا أتكلم على العملية اللي تستخدم
+
+98
+00:07:27,360 --> 00:07:31,160
+فيها المواد الكيميائية بحكي Antimicrobial
+
+99
+00:07:31,160 --> 00:07:34,520
+Chemotherapy يبقى الـ Antimicrobial Chemotherapy
+
+100
+00:07:34,520 --> 00:07:37,620
+هي العملية is the use of chemical هو استخدام
+
+101
+00:07:37,620 --> 00:07:41,780
+المواد الكيميائية to inhibit or kill microorganism
+
+102
+00:07:41,780 --> 00:07:44,960
+in or on the host الhost يعني الـ living tissue
+
+103
+00:07:44,960 --> 00:07:49,220
+يبقى هذا المصطلح والمصطلح اللي ذكرناه أخرش الـ
+
+104
+00:07:49,220 --> 00:07:52,800
+Chemotherapeutic agentهم تقريبًا نفس المصطلح لكن
+
+105
+00:07:52,800 --> 00:07:55,680
+الـ Chemotherapeutic Agent عبارة عن كيميكا اللي هي
+
+106
+00:07:55,680 --> 00:07:59,160
+المواد الكيميائية نفسها لكن الـ Antimicrobial
+
+107
+00:07:59,160 --> 00:08:02,300
+Chemotherapy أنا بتكلم عن العملية اللي أنا بستخدم
+
+108
+00:08:02,300 --> 00:08:07,560
+فيها المواد الكيميائية لتثبيت أو قتل ال micro
+
+109
+00:08:07,560 --> 00:08:12,320
+organism in or on the host or living tissue طيب لو
+
+110
+00:08:12,320 --> 00:08:16,880
+أنا بدي أقسم الـ Antimicrobial Chemotherapeutic
+
+111
+00:08:16,880 --> 00:08:23,810
+Agent بنقسمهم لصنفينأول صنف اللي هو الـ Antibiotic
+
+112
+00:08:23,810 --> 00:08:27,430
+الـ Antibiotic substance produced as metabolic
+
+113
+00:08:27,430 --> 00:08:32,090
+product of one microorganism which inhibit or kill
+
+114
+00:08:32,090 --> 00:08:38,310
+other microorganism هي تأخذ من كائن حي حتى يؤثر
+
+115
+00:08:38,310 --> 00:08:43,210
+على كائن حي آخر باخدها من العمليات الأيضية حتى
+
+116
+00:08:43,210 --> 00:08:48,230
+يؤثر عليها من خلال التصبيت أو القتل على ال living
+
+117
+00:08:48,230 --> 00:08:53,200
+tissue زي ما ذكرنالكن بالنسبة لمصطلح التاني الـ
+
+118
+00:08:53,200 --> 00:08:56,540
+Antimicrobial Chemotherapeutic Chemical Chemical
+
+119
+00:08:56,540 --> 00:09:01,860
+هي كيميكالز بيتم إنتاجها في المختبر in laboratory
+
+120
+00:09:01,860 --> 00:09:06,580
+المهان بيتم إنتاجها أو بيتم استخلاصها من كائنات
+
+121
+00:09:06,580 --> 00:09:10,000
+حية لكن الـ Antimicrobial Chemotherapeutic
+
+122
+00:09:10,000 --> 00:09:14,520
+Chemical هي مواد كيميائية بيتم تصنيعهافي المختبرات
+
+123
+00:09:14,520 --> 00:09:19,800
+حتى تأثر على الـ microorganism إما بالتفبيت أو في
+
+124
+00:09:19,800 --> 00:09:26,080
+القطن هذا بالنسبة للفرق بينهم بالنسبة للـ
+
+125
+00:09:26,080 --> 00:09:29,420
+Antimicrobial chemotherapy هي العملية لكن لما
+
+126
+00:09:29,420 --> 00:09:34,020
+بنتكلم على ال chemical نفسها بنقول ال chemotherapy
+
+127
+00:09:34,020 --> 00:09:39,680
+agentالـ Chemotherapeutic Agents بيصنفوا لصنفين
+
+128
+00:09:39,680 --> 00:09:42,540
+اللي هم الـ Antibiotic وAntimicrobial
+
+129
+00:09:42,540 --> 00:09:46,100
+Chemotherapeutic Chemicals الـ Antibiotic هي مواد
+
+130
+00:09:46,100 --> 00:09:50,440
+من كائنات حية تستخلص من كائن حي لكن الـ
+
+131
+00:09:50,440 --> 00:09:55,000
+Antimicrobial Chemotherapeutic Chemicals يتم
+
+132
+00:09:55,000 --> 00:09:56,760
+تصنيعها في المختبر
+
+133
+00:10:00,140 --> 00:10:03,300
+في الـ slide اللي قبل صنفنا الـ Antimicrobial
+
+134
+00:10:03,300 --> 00:10:07,720
+Agent حسب الـ origin حسب المصدر لـ Antibiotic و
+
+135
+00:10:07,720 --> 00:10:11,040
+Antimicrobial Chemotherapeutic Chemical لأنه قلنا
+
+136
+00:10:11,040 --> 00:10:14,740
+أن الـ Antibiotic تستخلص من كائن حي لكن الـ
+
+137
+00:10:14,740 --> 00:10:18,560
+Antimicrobial Chemotherapeutic Chemical كانت من
+
+138
+00:10:18,560 --> 00:10:22,680
+المختبر يتم تصنيحها في المختبر هلأ بنصنف الـ
+
+139
+00:10:22,680 --> 00:10:27,120
+Antimicrobial Agent حسب الـ action حسب الـ action
+
+140
+00:10:27,120 --> 00:10:32,750
+تصنف إلى اتنينوstatic شو يعني sideal؟ sideal اللي
+
+141
+00:10:32,750 --> 00:10:36,590
+هو the kill microorganism اللي بتعمل قتل لل micro
+
+142
+00:10:36,590 --> 00:10:40,090
+organism لأن احنا اتفقنا ان ال antimicrobial agent
+
+143
+00:10:40,090 --> 00:10:45,230
+مصطلح عام يستخدم لقتل او تفضيل فلو كان ال action
+
+144
+00:10:45,230 --> 00:10:49,510
+sideal يبقى أنا بتكلم على ال kill microorganism في
+
+145
+00:10:49,510 --> 00:10:54,210
+عندي أمثلة على antibiotic تستخدم في قتل ال micro
+
+146
+00:10:54,210 --> 00:10:58,550
+organism بنسلين، سفالوسبورين، ستربتميسين،
+
+147
+00:10:58,550 --> 00:11:03,790
+نيموميسينهذا بالنسبة لـ Stidal بالنسبة للـ Static
+
+148
+00:11:03,790 --> 00:11:07,430
+اللي هي Inhibit microbial growth long enough for
+
+149
+00:11:07,430 --> 00:11:10,830
+the body's own defense to remove the organism
+
+150
+00:11:10,830 --> 00:11:16,310
+بتعمل على تثبيت نمو الكائن الحي من الأمثل على الـ
+
+151
+00:11:16,310 --> 00:11:20,350
+Antibiotic أو الـ Antimicrobial agent اللي static
+
+152
+00:11:20,350 --> 00:11:25,950
+in action Tetracycline erythromycin sulfonamide
+
+153
+00:11:26,090 --> 00:11:30,270
+هدول كلهم Antimicrobial Agent بالنسبة لـ Action
+
+154
+00:11:30,270 --> 00:11:34,070
+Static بتعمل Inhibition للميكروبيا لجراسه لكن
+
+155
+00:11:34,070 --> 00:11:37,810
+بالنسبة لـ Antimicrobial Agent اللي بتعمل قتل
+
+156
+00:11:37,810 --> 00:11:41,330
+Cydal in Action بنسلن، Cephalosporin،
+
+157
+00:11:41,330 --> 00:11:45,890
+Streptomycin، Neomycin هدول كلهم حفظ مطلوب انكوا
+
+158
+00:11:45,890 --> 00:11:50,130
+حفظ شهو الـ Antimicrobial Agent اللي بتعمل Cydal
+
+159
+00:11:50,130 --> 00:11:53,840
+in Action ومين اللي بتعمل Static in Actionطب نيجي
+
+160
+00:11:53,840 --> 00:11:58,140
+لمصطلح مهم اللي هو selective toxicity selective
+
+161
+00:11:58,140 --> 00:12:02,720
+toxicity شوها ده المصطلح معناه السمية الإختيارية
+
+162
+00:12:02,720 --> 00:12:07,360
+بيسميها that the agent used must inhibit or kill
+
+163
+00:12:07,360 --> 00:12:11,440
+the microorganism without seriously harming the
+
+164
+00:12:11,440 --> 00:12:16,480
+host بقوللي إن ال chemicals أو الـ antimicrobial
+
+165
+00:12:16,480 --> 00:12:19,380
+agent اللي بيعمل inhibition or kill ال micro
+
+166
+00:12:19,380 --> 00:12:24,170
+organismلازم أنه يعمل هذا الـ Action يثبت أو يقتل
+
+167
+00:12:24,170 --> 00:12:28,290
+الميكروب من غير ما يأثر على الـ Host على العائل
+
+168
+00:12:28,290 --> 00:12:33,290
+بأي أدرار هذا بالنسبة لـ Selective Toxicity أنه
+
+169
+00:12:33,290 --> 00:12:37,670
+الـ Antimicrobial Agent لازم يثبت أو يقتل الميكروب
+
+170
+00:12:37,670 --> 00:12:43,310
+من غير ما يأثر على العائل بأي أدرار طبيعي طبعا أنه
+
+171
+00:12:43,310 --> 00:12:47,690
+يكون فيه side effect أثر جانبية لكن لازم أنها تكون
+
+172
+00:12:47,690 --> 00:12:51,630
+غير مؤذية للعائلهذا بالنسبة لتصنيف الـ
+
+173
+00:12:51,630 --> 00:12:58,490
+Antimicrobial Agent حسب الـ Action قلنا
+
+174
+00:12:58,490 --> 00:13:02,190
+إن الـ Antimicrobial Agent بصنفه حسب أول شيء الـ
+
+175
+00:13:02,190 --> 00:13:05,930
+Origin لـ Antibiotic و Antimicrobial كيموثيربيوتك
+
+176
+00:13:05,930 --> 00:13:10,710
+كيميكال حسب الـ Action لـ Cydal و Static حسب، هلأ
+
+177
+00:13:10,710 --> 00:13:14,950
+نيجي لـ Spectrum حسب المادة أو المجال اللي بتأثر
+
+178
+00:13:14,950 --> 00:13:19,320
+عليههي الـ Antimicrobial agent من صنفه لـ Broad
+
+179
+00:13:19,320 --> 00:13:23,580
+Spectrum و ل Narrow Spectrum بالنسبة لـ Broad
+
+180
+00:13:23,580 --> 00:13:28,680
+Spectrum اللي هو واسع الطيف Drugs which are
+
+181
+00:13:28,680 --> 00:13:32,080
+effective agents of a variety of both Gram
+
+182
+00:13:32,080 --> 00:13:35,300
+-positive and Gram-negative bacteria من اسمه واسع
+
+183
+00:13:35,300 --> 00:13:38,900
+الطيف فبالتالي بيأثر على كل من ال Gram-positive
+
+184
+00:13:38,900 --> 00:13:42,640
+and Gram-negative bacteriaمن الأمثلة على الـ
+
+185
+00:13:42,640 --> 00:13:46,060
+Antimicrobial Agents اللي هي الـ Broad Spectrum
+
+186
+00:13:46,060 --> 00:13:50,860
+وهدول حفظ تتراسيكلينستربتومايسين سيفالوسبورين
+
+187
+00:13:50,860 --> 00:13:56,120
+أمبيسيلين سالفون أماي هدول أمثلة على Antimicrobial
+
+188
+00:13:56,120 --> 00:14:00,000
+Agents تعتبر Broad Spectrum طيب، بالنسبة للـ
+
+189
+00:14:00,000 --> 00:14:03,020
+Narrow Spectrum طبعا قلنا تصنيفان Antimicrobial
+
+190
+00:14:03,020 --> 00:14:06,160
+Agents حسب الـ Spectrum لـ Broad و Narrow عرفنا شو
+
+191
+00:14:06,160 --> 00:14:12,610
+يعني Broad Narrow من معناها ضيقهيأثر على نوع معين
+
+192
+00:14:12,610 --> 00:14:16,010
+من البكتيريا على مجموعة معينة drug which are
+
+193
+00:14:16,010 --> 00:14:20,130
+effective against just gram positive bacteria or
+
+194
+00:14:20,130 --> 00:14:23,790
+just gram negative bacteria or only a few species
+
+195
+00:14:23,790 --> 00:14:27,810
+يبقى هي بتأثر فقط على ال gram negative أو ممكن
+
+196
+00:14:27,810 --> 00:14:30,430
+أنها تأثر فقط على ال gram positive أو ممكن أنها
+
+197
+00:14:30,430 --> 00:14:35,410
+تأثر فقط على a few speciesمن الأمثلة على البكتيريا
+
+198
+00:14:35,410 --> 00:14:38,530
+اللى أو من الأمثلة على الـ Antimicrobial agent
+
+199
+00:14:38,530 --> 00:14:42,330
+اللى تعتبر narrow spectrum اللى هى الـ penicillin
+
+200
+00:14:42,330 --> 00:14:46,190
+الاريثروميسين الـ glendamycin والجنتاميسين هدول
+
+201
+00:14:46,190 --> 00:14:50,830
+كلهم يعتبروا narrow spectrum طيب مين أفضل ال
+
+202
+00:14:50,830 --> 00:14:54,450
+narrow spectrum او ال broad spectrum بقوللي ان ال
+
+203
+00:14:54,450 --> 00:14:59,170
+narrow spectrum هو الأفضل ليش هو الأفضل أول سبب
+
+204
+00:14:59,170 --> 00:15:03,760
+اللى هو مش مذكورلأنه more specific مختص بشكل أكتر
+
+205
+00:15:03,760 --> 00:15:08,460
+لأنه بيستهدف نوع معين هذا بالنسبة لأول سبب السبب
+
+206
+00:15:08,460 --> 00:15:12,740
+التاني بيقوللي انه الـ Neurospectrum it will cause
+
+207
+00:15:12,740 --> 00:15:16,760
+less distraction to the body to the body's normal
+
+208
+00:15:16,760 --> 00:15:21,260
+efflora قبل ما نذكر أو نحكي عن هذا السبب خلينا
+
+209
+00:15:21,260 --> 00:15:26,340
+نعرف شو هي ال normal efflora ال normal efflora
+
+210
+00:15:26,340 --> 00:15:32,160
+بتكون بكتيريا مفيدةعلى سبيل المثال مثلا في ال ..
+
+211
+00:15:32,160 --> 00:15:35,680
+ال .. ال .. في أي مكان في الجسم بيكون فيه عندي
+
+212
+00:15:35,680 --> 00:15:40,240
+بكتيريا مفيدة بيكون عاملة زي خط دفع لو .. لو أنا
+
+213
+00:15:40,240 --> 00:15:44,640
+أعطيت المريض Broad Spectrum يعني Antimicrobial
+
+214
+00:15:44,640 --> 00:15:48,320
+Agent بيكون واسع التأثير بيأثر على الـ Gram
+
+215
+00:15:48,320 --> 00:15:52,340
+Positive والـ Gram Negative بكتيريا إيش هيصير؟ رح
+
+216
+00:15:52,340 --> 00:15:56,240
+يقتل هذا الـ Antimicrobial Agent رح يقتل أو يثبت
+
+217
+00:15:56,240 --> 00:16:02,070
+حسب اللي هو شنوهرح يعمل على قتل أو تثبيت الميكروب
+
+218
+00:16:02,070 --> 00:16:06,590
+والـ Normal flora كمان فبالتالي راح تروح البكتيريا
+
+219
+00:16:06,590 --> 00:16:11,490
+الانتهازية الـ abortionistic microorganism تعمل
+
+220
+00:16:11,490 --> 00:16:16,090
+super infection طبعا، طيب، هلأ لو أنا استخدمت
+
+221
+00:16:16,090 --> 00:16:19,370
+narrow spectrum هذا الاشي مش هيصير فبالتالي will
+
+222
+00:16:19,370 --> 00:16:22,750
+cause less destruction to the body's normal flora
+
+223
+00:16:23,520 --> 00:16:27,840
+هيعمل إنه هيكون أقل ضرر لبكتيريا الـ normal flora
+
+224
+00:16:27,840 --> 00:16:32,860
+هاي بالتالي هيحافظ عليها هتظلها تعمل الوظائف
+
+225
+00:16:32,860 --> 00:16:37,560
+المختلفة لإلها طيب، هنكتب لإن سوء استخدام أو
+
+226
+00:16:37,560 --> 00:16:41,200
+استخدام للـ broad-spectrum antibiotic can lead to
+
+227
+00:16:41,200 --> 00:16:43,840
+super infection بيقدي لsuper infection by
+
+228
+00:16:43,840 --> 00:16:46,740
+abortionistic microorganism شو يعني super
+
+229
+00:16:46,740 --> 00:16:50,400
+infection يعني؟نتيجة خلل معين على سبيل المثال
+
+230
+00:16:50,400 --> 00:16:54,500
+استخدام Antibiotic R Antimicrobial Agent Broad
+
+231
+00:16:54,500 --> 00:16:58,780
+Spectrum راح تقضي على الـNormaliflora وعلى
+
+232
+00:16:58,780 --> 00:17:02,640
+الميكروب راح تيجي البكتيريا الانتهازية وتسبب لإله
+
+233
+00:17:02,640 --> 00:17:06,300
+عدوى هذه العدوى بسميها Super Infection لأن هو
+
+234
+00:17:06,300 --> 00:17:11,180
+بالأساس ما كان له عدوى لكن نتيجة اللي هو استخدام
+
+235
+00:17:11,180 --> 00:17:15,120
+الـBroad Spectrum Antimicrobial Agent صار هذا
+
+236
+00:17:15,120 --> 00:17:18,970
+الأشياءلكن لو أنا بتكلم على الـ Primary Infection
+
+237
+00:17:18,970 --> 00:17:23,990
+بتكون العدوى ابتجيني من طبعا من بكتيريا ممرضة
+
+238
+00:17:23,990 --> 00:17:28,810
+وبتسبب المرض هذا بالنسبة لآخر تصنيف من تصنيفات الـ
+
+239
+00:17:28,810 --> 00:17:32,610
+Antimicrobial Agent حسب الـ Spectrum ومطلوب حفظ
+
+240
+00:17:32,610 --> 00:17:35,530
+اللي هو الأمثلة على الـ Broad Spectrum و Narrow
+
+241
+00:17:35,530 --> 00:17:39,750
+Spectrum واخر شئ لازم نعرف ليش أنا أو شو بفضل أكيد
+
+242
+00:17:39,750 --> 00:17:42,990
+بنفضل الـ Narrow Spectrum لأنه more specific تاني
+
+243
+00:17:42,990 --> 00:17:46,870
+الشيء لأنهwill cause less destruction to the
+
+244
+00:17:46,870 --> 00:17:50,830
+body's normal flora لو أنا استخدمت الـ Broad
+
+245
+00:17:50,830 --> 00:17:53,990
+Spectrum Antimicrobial Agent هيسببلي super
+
+246
+00:17:53,990 --> 00:17:58,150
+infection by abortionistic microorganism وعرفنا شو
+
+247
+00:17:58,150 --> 00:18:01,710
+يعني super infection وشو يعني primary infection
+
+248
+00:18:05,420 --> 00:18:09,280
+في هاي الـ slide هنتكلم على مصطلحات مهمة هو
+
+249
+00:18:09,280 --> 00:18:13,280
+المصطلح الـ antibiotic-resistant اللي هي مقاومة
+
+250
+00:18:13,280 --> 00:18:18,160
+المضادات الحيوية bacteria gain ability to grow no
+
+251
+00:18:18,160 --> 00:18:22,760
+longer sensitive to a drug إنه البكتيريا بيصير
+
+252
+00:18:22,760 --> 00:18:27,460
+عندها القدرة إنها تنمو حتى بوجود المضاد الحيوي
+
+253
+00:18:27,460 --> 00:18:31,860
+بطلت تتأثر في الـ antimicrobial agent
+
+254
+00:18:33,080 --> 00:18:37,400
+Antiretroviral هي عبارة عن مضادات بتشتغل ضد
+
+255
+00:18:37,400 --> 00:18:41,560
+الفيروسات طب نيجي ل ال combination of a drug اللي
+
+256
+00:18:41,560 --> 00:18:45,260
+هو اتحاد الأدوية فيه عندي تلت مصطلحات مهمة بالنسبة
+
+257
+00:18:45,260 --> 00:18:49,960
+ل ال combination of a drug synergism، antagonism و
+
+258
+00:18:49,960 --> 00:18:54,820
+additive بالنسبة لأول واحد اللي هو synergism
+
+259
+00:18:54,820 --> 00:18:58,860
+synergism يعني action of two antibiotic greater
+
+260
+00:18:58,860 --> 00:19:04,630
+مجموع تأثيرفعالية المضادات الحيوية لإتنين أكبر من
+
+261
+00:19:04,630 --> 00:19:10,070
+تأثير كل مضاد حيوي على حدا يعني واحد زائد واحد
+
+262
+00:19:10,070 --> 00:19:13,350
+بيكون مش بيساوي اتنين أكبر من اتنين لأن مجموع
+
+263
+00:19:13,350 --> 00:19:17,810
+تأثير فعليتهم مع بعض أكبر من تأثير كل مضاد حيوي
+
+264
+00:19:17,810 --> 00:19:22,650
+على حدا فبالتالي في هاي الحالة ميح انه ناخد هدول
+
+265
+00:19:22,650 --> 00:19:27,230
+الأدوية مع بعض لأن تأثيرهم مع بعض أكبر من تأثير كل
+
+266
+00:19:27,230 --> 00:19:31,980
+مضاد حيوي على حدابالنسبة لـ Antagonism اللي هو
+
+267
+00:19:31,980 --> 00:19:36,080
+action of drug is reduced less effective إنه مجموع
+
+268
+00:19:36,080 --> 00:19:40,060
+تأثير الـ two drug مع بعض أقل من تأثير كل واحد على
+
+269
+00:19:40,060 --> 00:19:45,220
+حدا يعني واحد زائد واحد بيكون أقل من اتنين أخر
+
+270
+00:19:45,220 --> 00:19:49,300
+شائل هي ال additive the action of two drugs in
+
+271
+00:19:49,300 --> 00:19:52,140
+combination is equal to the sum of their
+
+272
+00:19:52,140 --> 00:19:55,540
+independent activity when studied separatelyبقول
+
+273
+00:19:55,540 --> 00:19:59,880
+لي إن مجموع تأثير المضادين الحيويين بيساوي تأثير
+
+274
+00:19:59,880 --> 00:20:03,680
+كل مضاد حيوي على حد يعني واحد زائد واحد بيساوي
+
+275
+00:20:03,680 --> 00:20:08,220
+اتنين هذا بالنسبة لـ Combination of a Drug نيجي
+
+276
+00:20:08,220 --> 00:20:12,180
+لمصطلحين مهمين اللي هم الـ Minimum Inhibitory
+
+277
+00:20:12,180 --> 00:20:18,200
+Concentration MIC The lowest concentration of
+
+278
+00:20:18,200 --> 00:20:22,120
+chemotherapy agent capable of preventing growth of
+
+279
+00:20:22,120 --> 00:20:27,140
+the test organismهو أقل تركيز من الـ Antimicrobial
+
+280
+00:20:27,140 --> 00:20:31,740
+agent قادر على منع نمو البكتيريا شو منع نمو
+
+281
+00:20:31,740 --> 00:20:34,900
+البكتيريا، شو معناها، اللي هي تفضيط، Inhibition
+
+282
+00:20:34,900 --> 00:20:39,200
+عشان هيك أنا قولت Minimum inhibitory يعني هو أقل
+
+283
+00:20:39,200 --> 00:20:42,160
+تركيز من الـ Antimicrobial agent قادر إنه يعمل
+
+284
+00:20:42,160 --> 00:20:46,200
+Inhibition للبكتيريا Preventing the growth يعني
+
+285
+00:20:46,200 --> 00:20:50,640
+يعمل Inhibition لكن الـ Minimum bactericidal
+
+286
+00:20:50,640 --> 00:20:54,650
+concentrationاللي هو the lowest concentration of
+
+287
+00:20:54,650 --> 00:20:59,850
+chemotherapy agent ذات result is in no growth اللي
+
+288
+00:20:59,850 --> 00:21:05,230
+هي turbidity of subculture هو أقل تركيز من الـ
+
+289
+00:21:05,230 --> 00:21:09,830
+antibiotic أو الـ antimicrobial agent خاطر على قتل
+
+290
+00:21:09,830 --> 00:21:12,770
+البكتيريا، إنه يكون فيه عندي no growth no growth
+
+291
+00:21:12,770 --> 00:21:17,030
+يعني قتل البكتيريا، إن ما يكون فيه عندي بكتيريا في
+
+292
+00:21:17,030 --> 00:21:21,940
+الـ Lithiumأو ممكن نحكي في تعريف أدق اللي هو أقل
+
+293
+00:21:21,940 --> 00:21:26,280
+تركيز من الـ Antimicrobial agent بيسمح بنمو أقل من
+
+294
+00:21:26,280 --> 00:21:31,520
+1 من 10% من الميكروب هذا بالنسبة لـ Minimum
+
+295
+00:21:31,520 --> 00:21:34,720
+Inhibitory Concentration والـ Minimum Bacterioidal
+
+296
+00:21:34,720 --> 00:21:38,380
+Concentration الفصل الجاي في مساق الـ Diagnostic
+
+297
+00:21:38,380 --> 00:21:41,780
+Medical Microbiology هتتعرفوا بالتفصيل كيف أنا بدي
+
+298
+00:21:41,780 --> 00:21:46,020
+أحسب الـ Minimum Inhibitory Concentration والـ
+
+299
+00:21:46,020 --> 00:21:48,300
+Minimum Bacterioidal Concentration
+
+300
+00:21:53,540 --> 00:21:58,120
+Antimicrobial Activity كيف تأثير أو كيف بيأثر الـ
+
+301
+00:21:58,120 --> 00:22:02,580
+Antimicrobial Agent على الخلية في عندي خمس طرق of
+
+302
+00:22:02,580 --> 00:22:08,680
+Antimicrobial Activity هنذكرهم بشكل مختصر الفصل
+
+303
+00:22:08,680 --> 00:22:11,520
+الجاي في مادة الـ Diagnostic Medical Microbiology
+
+304
+00:22:11,520 --> 00:22:16,300
+هيكون فيه chapter كامل على هذا الموضوع يعني هيكون
+
+305
+00:22:16,300 --> 00:22:21,530
+فيه تفاصيل أكتر لكن في هذا الفصل فقط هنذكربعض
+
+306
+00:22:21,530 --> 00:22:26,450
+المعلومات الأساسية بالنسبة لأول طريقة Injury to
+
+307
+00:22:26,450 --> 00:22:29,490
+plasma membrane ممكن أنه الـ Antimicrobial agent
+
+308
+00:22:29,490 --> 00:22:32,050
+يأثر على الـ plasma membrane مثال على
+
+309
+00:22:32,050 --> 00:22:36,330
+Antimicrobial agent الـ Polymyxin B هو هذا بيأثر
+
+310
+00:22:36,330 --> 00:22:39,730
+على ال plasma membrane ممكن أنه يعمل Inhibition of
+
+311
+00:22:39,730 --> 00:22:43,850
+cell wall synthesisمثال عليا الـ Penicillin و الـ
+
+312
+00:22:43,850 --> 00:22:48,650
+Bacitracin وإحنا قولنا في أول معامل في المادة إن
+
+313
+00:22:48,650 --> 00:22:51,810
+الـ Penicillin بيعمل على تفكيك الرابط اللي كانت
+
+314
+00:22:51,810 --> 00:22:55,610
+بين الـBacterial glycan فبالتالي بيعمل Inhibition
+
+315
+00:22:55,610 --> 00:22:59,470
+للـ Cell Wall Synthesis ممكن يؤثر على الـ Protein
+
+316
+00:22:59,470 --> 00:23:03,950
+Synthesis بالتحديد اللي هي الـ Translation ممكن
+
+317
+00:23:03,950 --> 00:23:07,030
+يؤثر يعمل Inhibition of Nucleic Acid Replication
+
+318
+00:23:07,030 --> 00:23:10,930
+أو Retranscriptionممكن يعمل Inhibition of
+
+319
+00:23:10,930 --> 00:23:13,950
+Essential Metabolites هذا بالنسبة للـ
+
+320
+00:23:13,950 --> 00:23:19,290
+Antimicrobial Activity بالنسبة
+
+321
+00:23:19,290 --> 00:23:21,950
+للـ Antimicrobial Activity قولنا ممكن أنه يعمل
+
+322
+00:23:21,950 --> 00:23:25,870
+Injury للـ Plasma Membrane مثلًا عليه البولي
+
+323
+00:23:25,870 --> 00:23:30,410
+ميكسين بيه ممكن يعمل Inhibition للسل والـ
+
+324
+00:23:30,410 --> 00:23:32,990
+Synthesis قولنا في أمثلة الـ Penicillin،
+
+325
+00:23:32,990 --> 00:23:36,770
+الستفالوسبورين، البسترسين، الفانكمايسينممكن يعمل
+
+326
+00:23:36,770 --> 00:23:40,170
+Inhibition لـ Protein Synthesis مثال عليه بالأخص
+
+327
+00:23:40,170 --> 00:23:43,890
+اللي هو Translation لـ Chloramphenicol لـ
+
+328
+00:23:43,890 --> 00:23:47,470
+Erythromycin لـ Tetracycline لـ Streptomycin ممكن
+
+329
+00:23:47,470 --> 00:23:52,230
+يؤثر على اللي هو Inhibition of Synthesis of
+
+330
+00:23:52,230 --> 00:23:56,990
+Essential Metabolites ممكن يؤثر على اللي هو لـ
+
+331
+00:23:56,990 --> 00:24:00,450
+Nucleic Acid Replication and Transcription طبعا
+
+332
+00:24:00,450 --> 00:24:04,130
+احنا بس بناعرف اللي هو شو الـ Active شو الـ
+
+333
+00:24:04,130 --> 00:24:07,740
+Antimicrobial Activityالأمثلة في هاي الـ slide مش
+
+334
+00:24:07,740 --> 00:24:12,880
+مطلوب حفظ الفصل الجاي هتكون المادة في التفصيل لكن
+
+335
+00:24:12,880 --> 00:24:18,860
+احنا في هذا الفصل بس بدنا نعرف شو الطرق أو كيف الـ
+
+336
+00:24:18,860 --> 00:24:24,060
+Antimicrobial agent بيأثر على الخلية البكتيرية
+
+337
+00:24:24,060 --> 00:24:28,180
+مطلوب فقط حفظ اللي تم ذكره في ال slide اللي قبل
+
+338
+00:24:28,180 --> 00:24:33,030
+فقط أما في هاي ال slide فقط بدنا نعرف شوطرق بشكل
+
+339
+00:24:33,030 --> 00:24:37,290
+مختصر كيف بيأثر الـ Antimicrobial agent على الخلية
+
+340
+00:24:37,290 --> 00:24:41,850
+البكتيرية بالقتل أو بالتثبيت بدون حفظ الأمثلة لكن
+
+341
+00:24:41,850 --> 00:24:49,610
+مطلوب حفظ ما تم ذكره في ال slide اللي قبل How
+
+342
+00:24:49,610 --> 00:24:53,370
+to choose antimicrobial drug عوامل لازم أخدها بعين
+
+343
+00:24:53,370 --> 00:24:58,770
+الاعتبار قبل وصف أي antimicrobial drugهل هو Narrow
+
+344
+00:24:58,770 --> 00:25:02,970
+Spectrum أو Broad Spectrum قلنا يفضل إن أنا أستخدم
+
+345
+00:25:02,970 --> 00:25:06,190
+الـ Narrow Spectrum للأسباب اللي ذكرناها سابقة
+
+346
+00:25:06,190 --> 00:25:10,430
+ممكن إننا نستخدم الـ Broad Spectrum في حالات
+
+347
+00:25:10,430 --> 00:25:15,770
+إستثنائية هل المريض Old Age أو Child نعرف إنه الـ
+
+348
+00:25:15,770 --> 00:25:19,730
+Antibiotic فيه تركيز مختلفة فأكيد مش هعطي الـ
+
+349
+00:25:19,730 --> 00:25:23,810
+Child نفس تركيز الـ Old Age Male أو Female
+
+350
+00:25:23,810 --> 00:25:29,600
+Pregnant أو Lactating Womeninpatient or outpatient
+
+351
+00:25:29,600 --> 00:25:34,360
+inpatient اللي هو مريض داخل المشفة بيصرفوله برد
+
+352
+00:25:34,360 --> 00:25:38,680
+spectrum لأنه بيكون معرض للتعرض للكثير من
+
+353
+00:25:38,680 --> 00:25:44,480
+الميكروبات لكن ال outpatient اللي هو خارج المشفة
+
+354
+00:25:44,480 --> 00:25:49,200
+type of microorganism نوع ال micro organism site
+
+355
+00:25:49,200 --> 00:25:54,730
+of infection اللي هي مكان العدوىممكن تكون سطحية
+
+356
+00:25:54,730 --> 00:26:01,230
+فبيتم صرف مضاد حيوي على شكل برهم لكن لو كان داخل
+
+357
+00:26:01,230 --> 00:26:06,890
+الجسم بيتم إعطاءه capsule أو injection هذا بالنسبة
+
+358
+00:26:06,890 --> 00:26:10,970
+للعوامل اللي لازم أخدها بعين الاعتبار عند وصف أي
+
+359
+00:26:10,970 --> 00:26:18,650
+مضاد حيوي هاي
+
+360
+00:26:18,650 --> 00:26:22,840
+ال slide من chapter الـ Serial Dilutionحاب أنوه
+
+361
+00:26:22,840 --> 00:26:27,280
+لبعض المعلومات اللي ما ذكرتها في الـ Chapter هلأ
+
+362
+00:26:27,280 --> 00:26:31,080
+بالنسبة للـ Pour Eplate شو قصده في الـ Pour Eplate
+
+363
+00:26:31,080 --> 00:26:35,280
+للـ Pour Eplate إنه أنا بحط العينة قبل ما أنا أصب
+
+364
+00:26:35,280 --> 00:26:40,280
+الميديا هيك أنا بوفر ظروف لا هواقية لكن الـ Spread
+
+365
+00:26:40,280 --> 00:26:44,400
+Eplate الـ Spread Eplate بحط بتكون مصبوبة الميديا
+
+366
+00:26:44,400 --> 00:26:48,880
+بعدين بحط العينة هيك أنا بوفر ظروف هواقية طيب ليش
+
+367
+00:26:48,880 --> 00:26:50,660
+في ال slide ذاكرها
+
+368
+00:26:53,960 --> 00:26:58,420
+عشرة أساليب تلاتة وأخد من هان اللي هو من الـ
+
+369
+00:26:58,420 --> 00:27:01,780
+dilution عشرة أساليب تلاتة هان أخد من ال dilution
+
+370
+00:27:01,780 --> 00:27:06,000
+أو من الـ tube التخفيف تبعها عشرة أساليب اتنين
+
+371
+00:27:06,000 --> 00:27:10,180
+وذكر عشرة أساليب تلاتة أحنا قولنا في القانون الـ
+
+372
+00:27:10,180 --> 00:27:14,460
+concentration اللي هو الـ number of colony عدد ال
+
+373
+00:27:14,460 --> 00:27:20,040
+colony اللي موجودة على الطبق على الـ S اللي هي الـ
+
+374
+00:27:20,040 --> 00:27:24,110
+volume اللي أناأخدته من هايل يوتيوب وفردته أو
+
+375
+00:27:24,110 --> 00:27:29,990
+حطيته على البلاد ضرب الـ Dilution هذا الرقم هو
+
+376
+00:27:29,990 --> 00:27:35,590
+حاصل ضرب الـ Dilution مع الـ S الـ S ضرب اللي هو
+
+377
+00:27:35,590 --> 00:27:42,390
+الـ Dilution هذا الرقم هان أخدت واحد ملي من هايل
+
+378
+00:27:42,390 --> 00:27:45,710
+يوتيوب فانا ضربتها في تخفيفة اللي هي عشرة أسالب
+
+379
+00:27:45,710 --> 00:27:51,300
+تلاتة فطلع عشرة أسالب تلاتة لكن هانأخد واحد من
+
+380
+00:27:51,300 --> 00:27:58,000
+عشرة ML ضرب اللي هي العشرة أسالب اتنين فطلعت
+
+381
+00:27:58,000 --> 00:28:01,960
+العشرة أسالب تلاتة هذا بالنسبة للرقم ليش هان مكتوب
+
+382
+00:28:01,960 --> 00:28:06,140
+عشرة أسالب تلاتة هي الرقم هذا حاصل ضرب ال dilution
+
+383
+00:28:06,140 --> 00:28:11,160
+مع ال S هان أخدت واحد من عشرة لكن هان أخدت واحد ML
+
+384
+00:28:11,160 --> 00:28:17,960
+لازم يكون الرقم اللي هو ال S لازم يكون بال ML لو
+
+385
+00:28:17,960 --> 00:28:23,220
+كان في ال microلازم أحاوله لـ ML هذه معلومة مهمة
+
+386
+00:28:23,220 --> 00:28:30,400
+لازم ننتبه لإلها في المثاقات هيك
+
+387
+00:28:30,400 --> 00:28:35,680
+بنكون وصلنا لنهاية شرح المحاضرة وأيضا لنهاية سلسلة
+
+388
+00:28:35,680 --> 00:28:40,040
+محاضرات مساق الـ Essential Medical Microbiology
+
+389
+00:28:40,040 --> 00:28:45,180
+بتمنعكم كل التوفيق والنجاح والسلام عليكم ورحمة
+
+390
+00:28:45,180 --> 00:28:46,400
+الله وبركاته
+
diff --git a/PL9fwy3NUQKwZWgAb_V1ATlRRsH-6i3cPC/wyOj5_y9CBQ_raw.json b/PL9fwy3NUQKwZWgAb_V1ATlRRsH-6i3cPC/wyOj5_y9CBQ_raw.json
new file mode 100644
index 0000000000000000000000000000000000000000..60b866a6156a3b06916b7dd1e36b3c845965d932
--- /dev/null
+++ b/PL9fwy3NUQKwZWgAb_V1ATlRRsH-6i3cPC/wyOj5_y9CBQ_raw.json
@@ -0,0 +1 @@
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"وكمان بعد ما انت من شغلي في المعمل ضروري انه ان احنا نعقم المكان وينطهره يبقى في عندي انا مصطلحين disinfection وفي عندي مصطلح تاني sterilization هدول المصطلحين بشكل مختصر الفرق بينهم ال disinfection معناه تطهير بدي اقتل فقط ال pathogenic bacteria", "tokens": [2407, 24793, 7649, 39182, 19446, 16472, 2655, 9154, 13412, 17082, 20292, 8978, 9673, 25957, 1211, 48812, 2288, 13063, 1829, 16472, 3224, 16472, 1975, 5016, 8315, 8717, 3615, 4587, 2304, 9673, 41361, 4032, 9957, 9566, 3224, 2288, 3224, 7251, 3555, 4587, 7578, 8978, 18871, 16254, 1975, 8315, 3714, 9381, 9566, 1211, 5016, 9957, 33334, 313, 4032, 41185, 18871, 16254, 3714, 9381, 9566, 1211, 5016, 6055, 7649, 1829, 41477, 2144, 8032, 3215, 12610, 9673, 9381, 9566, 1211, 5016, 9957, 4724, 8592, 28820, 3714, 46456, 9381, 2288, 27188, 2288, 4587, 49374, 16095, 2423, 33334, 313, 20449, 8315, 3224, 6055, 9566, 3224, 13546, 4724, 16254, 1975, 38149, 1211, 6156, 47432, 2423, 3100, 25473, 11763], "avg_logprob": -0.11282376072428248, 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"text": "وقولك الـ code لازم احنا نجيب كيس معمل الـ microbiology وكيس خاص ونحطه فيه ممنوع امسك ال lab code و احطه في الشنطة لأن خلال شغلك ما بتضمني ان يكون بكتيريا اجت على ال lab code روح تحطيها ال lab code في شنطك بالتالي هي كل الأدوات في الشنطة هتصير ملوثة بالبكتيريا وممكن انها تسبب infection لإلك Departing all injury accidents spells بقولنا", "tokens": [2407, 39648, 4117, 2423, 39184, 3089, 5296, 31377, 2304, 1975, 5016, 8315, 8717, 7435, 1829, 3555, 9122, 1829, 3794, 3714, 25957, 1211, 2423, 39184, 33234, 1793, 4032, 4117, 1829, 3794, 16490, 33546, 4032, 1863, 5016, 9566, 3224, 8978, 3224, 3714, 27842, 45367, 1975, 2304, 3794, 4117, 2423, 2715, 3089, 4032, 1975, 5016, 9566, 3224, 8978, 25124, 1863, 9566, 3660, 5296, 33456, 16490, 1211, 6027, 13412, 17082, 23275, 19446, 39894, 11242, 27842, 1829, 16472, 7251, 30544, 4724, 4117, 2655, 13546, 25528, 1975, 7435, 2655, 15844, 2423, 2715, 3089, 12602, 2407, 5016, 6055, 5016, 9566, 1829, 11296, 2423, 2715, 3089, 8978, 13412, 1863, 9566, 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1132.62, "end": 1132.96, "word": " Bag", "probability": 0.84375}, {"start": 1132.96, "end": 1133.22, "word": " الـ", "probability": 0.771484375}, {"start": 1133.22, "end": 1133.36, "word": " Auto", "probability": 0.75830078125}, {"start": 1133.36, "end": 1133.66, "word": "-Clap", "probability": 0.9637044270833334}, {"start": 1133.66, "end": 1133.88, "word": " Bag", "probability": 0.9619140625}, {"start": 1133.88, "end": 1134.06, "word": " هو", "probability": 0.8818359375}, {"start": 1134.06, "end": 1134.46, "word": " عبارة", "probability": 0.9970703125}, {"start": 1134.46, "end": 1134.68, "word": " عن", "probability": 0.99755859375}, {"start": 1134.68, "end": 1135.04, "word": " كيس", "probability": 0.98828125}, {"start": 1135.04, "end": 1136.14, "word": " هلأ", "probability": 0.6667277018229166}, {"start": 1136.14, "end": 1136.52, "word": " الـ", "probability": 0.6051025390625}, {"start": 1136.52, "end": 1137.22, "word": " Auto", "probability": 0.9091796875}, {"start": 1137.22, "end": 1137.52, "word": "-Clap", "probability": 0.9765625}, {"start": 1137.52, "end": 1137.72, "word": " Bag", "probability": 0.97021484375}, {"start": 1137.72, "end": 1138.16, "word": " منحط", "probability": 0.7370198567708334}, {"start": 1138.16, "end": 1138.44, "word": " فيها", "probability": 0.958984375}, {"start": 1138.44, "end": 1138.92, "word": " الأدوات", "probability": 0.979248046875}, {"start": 1138.92, "end": 1139.52, "word": " البلاستيك", "probability": 0.90732421875}, {"start": 1139.52, "end": 1139.72, "word": " زي", "probability": 0.972900390625}, {"start": 1139.72, "end": 1139.82, "word": " الـ", "probability": 0.85009765625}, {"start": 1139.82, "end": 1140.12, "word": " Petri", "probability": 0.921142578125}, {"start": 1140.12, "end": 1140.4, "word": " dish", "probability": 0.330078125}, {"start": 1140.4, "end": 1140.72, "word": " زي", "probability": 0.915771484375}, {"start": 1140.72, "end": 1140.86, "word": " الـ", "probability": 0.755126953125}, {"start": 1140.86, "end": 1141.1, "word": " Loops", "probability": 0.480712890625}, {"start": 1141.1, "end": 1141.4, "word": " لكن", "probability": 0.90478515625}, {"start": 1141.4, "end": 1142.06, "word": " الأدوات", "probability": 0.9901123046875}, {"start": 1142.06, "end": 1142.82, "word": " الحادة", "probability": 0.9568684895833334}, {"start": 1142.82, "end": 1143.5, "word": " زي", "probability": 0.9931640625}, {"start": 1143.5, "end": 1144.06, "word": " الـ", "probability": 0.9501953125}, {"start": 1144.06, "end": 1144.72, "word": " Needles،", "probability": 0.48486328125}, {"start": 1144.72, "end": 1145.56, "word": " Scalpels،", "probability": 0.770556640625}, {"start": 1145.56, "end": 1145.84, "word": " الـ", "probability": 0.6160888671875}, {"start": 1145.84, "end": 1146.3, "word": " Sharps", "probability": 0.6295166015625}, {"start": 1146.3, "end": 1146.8, "word": " هاي", "probability": 0.52978515625}, {"start": 1146.8, "end": 1147.16, "word": " كلها", "probability": 0.992431640625}, {"start": 1147.16, "end": 1147.74, "word": " بحطها", "probability": 0.9853515625}, {"start": 1147.74, "end": 1148.28, "word": " ضروري", "probability": 0.9312744140625}, {"start": 1148.28, "end": 1148.52, "word": " حتى", "probability": 0.9189453125}, {"start": 1148.52, "end": 1148.76, "word": " أني", "probability": 0.6280517578125}, {"start": 1148.76, "end": 1148.84, "word": " انا", "probability": 0.779052734375}, {"start": 1148.84, "end": 1149.34, "word": " أتخلص", "probability": 0.92578125}, {"start": 1149.34, "end": 1149.66, "word": " منها", "probability": 0.996826171875}, {"start": 1149.66, "end": 1150.22, "word": " بحطها", "probability": 0.9898681640625}, {"start": 1150.22, "end": 1150.52, "word": " في", "probability": 0.97509765625}, {"start": 1150.52, "end": 1151.3, "word": " Puncture", "probability": 0.4820556640625}, {"start": 1151.3, "end": 1152.28, "word": "-resistant", "probability": 0.6966145833333334}, {"start": 1152.28, "end": 1153.06, "word": " container", "probability": 0.6376953125}, {"start": 1153.06, "end": 1153.72, "word": " يعني", "probability": 0.958984375}, {"start": 1153.72, "end": 1154.22, "word": " وعاء", "probability": 0.94970703125}, {"start": 1154.22, "end": 1154.9, "word": " مقاوم", "probability": 0.996826171875}, {"start": 1154.9, "end": 1155.56, "word": " للخرق", "probability": 0.8924153645833334}, {"start": 1155.56, "end": 1155.88, "word": " لأني", "probability": 0.7718098958333334}, {"start": 1155.88, "end": 1156.02, "word": " انا", "probability": 0.789794921875}, {"start": 1156.02, "end": 1156.16, "word": " لو", "probability": 0.99267578125}, {"start": 1156.16, "end": 1156.8, "word": " حطيتها", "probability": 0.838134765625}, {"start": 1156.8, "end": 1157.44, "word": " للكيس", "probability": 0.87152099609375}], "temperature": 1.0}, {"id": 46, "seek": 117772, "start": 1157.64, "end": 1177.72, "text": "ممكن لو حطيت الـ needle في كيس اللي هو الـ autoclave bag ممكن تخرق الكيس وهيك بالتالي ممكن أنه تسبب أداء لإنسان اللي بيحملها عشان هيك الأدوات الحادة أنا بتخلص منها بحطها في puncture resistant container لكن الأدوات مثلا لبلاستيك بحطها في ال autoclave bag", "tokens": [2304, 43020, 45164, 11331, 9566, 36081, 2423, 39184, 11037, 8978, 9122, 1829, 3794, 13672, 1829, 31439, 2423, 39184, 45833, 27995, 3411, 3714, 43020, 6055, 9778, 2288, 4587, 33251, 1829, 3794, 37037, 1829, 4117, 20666, 2655, 6027, 1829, 3714, 43020, 14739, 3224, 6055, 35457, 3555, 5551, 3215, 16606, 5296, 28814, 1863, 3794, 7649, 13672, 1829, 4724, 1829, 35571, 1211, 11296, 6225, 8592, 7649, 39896, 4117, 16247, 3215, 2407, 9307, 21542, 18513, 3660, 41850, 39894, 9778, 1211, 9381, 9154, 11296, 4724, 5016, 9566, 11296, 8978, 27006, 540, 20383, 10129, 44381, 16247, 3215, 2407, 9307, 50113, 15040, 5296, 3555, 15040, 14851, 1829, 4117, 4724, 5016, 9566, 11296, 8978, 2423, 45833, 27995, 3411], "avg_logprob": -0.16349432170391082, "compression_ratio": 1.7575757575757576, "no_speech_prob": 3.337860107421875e-06, "words": [{"start": 1157.64, "end": 1158.26, "word": "ممكن", "probability": 0.939697265625}, {"start": 1158.26, "end": 1158.84, "word": " لو", "probability": 0.98388671875}, {"start": 1158.84, "end": 1159.34, "word": " حطيت", "probability": 0.97705078125}, {"start": 1159.34, "end": 1159.78, "word": " الـ", "probability": 0.665283203125}, {"start": 1159.78, "end": 1160.04, "word": " needle", "probability": 0.82470703125}, {"start": 1160.04, "end": 1160.34, "word": " في", "probability": 0.87451171875}, {"start": 1160.34, "end": 1160.94, "word": " كيس", "probability": 0.9503580729166666}, {"start": 1160.94, "end": 1161.12, "word": " اللي", "probability": 0.8291015625}, {"start": 1161.12, "end": 1161.28, "word": " هو", "probability": 0.99462890625}, {"start": 1161.28, "end": 1161.46, "word": " الـ", "probability": 0.910400390625}, {"start": 1161.46, "end": 1161.94, "word": " autoclave", "probability": 0.6534423828125}, {"start": 1161.94, "end": 1162.2, "word": " bag", "probability": 0.86474609375}, {"start": 1162.2, "end": 1162.66, "word": " ممكن", "probability": 0.974365234375}, {"start": 1162.66, "end": 1163.2, "word": " تخرق", "probability": 0.673583984375}, {"start": 1163.2, "end": 1163.86, "word": " الكيس", "probability": 0.9300130208333334}, {"start": 1163.86, "end": 1164.62, "word": " وهيك", "probability": 0.7283528645833334}, {"start": 1164.62, "end": 1165.08, "word": " بالتالي", "probability": 0.910888671875}, {"start": 1165.08, "end": 1165.4, "word": " ممكن", "probability": 0.9853515625}, {"start": 1165.4, "end": 1165.74, "word": " أنه", "probability": 0.795166015625}, {"start": 1165.74, "end": 1166.26, "word": " تسبب", "probability": 0.81591796875}, {"start": 1166.26, "end": 1166.66, "word": " أداء", "probability": 0.8258463541666666}, {"start": 1166.66, "end": 1167.52, "word": " لإنسان", "probability": 0.8615234375}, {"start": 1167.52, "end": 1167.68, "word": " اللي", "probability": 0.96435546875}, {"start": 1167.68, "end": 1168.18, "word": " بيحملها", "probability": 0.80107421875}, {"start": 1168.18, "end": 1168.56, "word": " عشان", "probability": 0.9488932291666666}, {"start": 1168.56, "end": 1169.3, "word": " هيك", "probability": 0.848876953125}, {"start": 1169.3, "end": 1169.92, "word": " الأدوات", "probability": 0.9730224609375}, {"start": 1169.92, "end": 1170.6, "word": " الحادة", "probability": 0.9049479166666666}, {"start": 1170.6, "end": 1170.84, "word": " أنا", "probability": 0.82568359375}, {"start": 1170.84, "end": 1171.34, "word": " بتخلص", "probability": 0.955078125}, {"start": 1171.34, "end": 1171.56, "word": " منها", "probability": 0.987060546875}, {"start": 1171.56, "end": 1171.98, "word": " بحطها", "probability": 0.9134521484375}, {"start": 1171.98, "end": 1172.12, "word": " في", "probability": 0.958984375}, {"start": 1172.12, "end": 1172.6, "word": " puncture", "probability": 0.5286102294921875}, {"start": 1172.6, "end": 1173.12, "word": " resistant", "probability": 0.85888671875}, {"start": 1173.12, "end": 1173.72, "word": " container", "probability": 0.93896484375}, {"start": 1173.72, "end": 1174.14, "word": " لكن", "probability": 0.91015625}, {"start": 1174.14, "end": 1174.78, "word": " الأدوات", "probability": 0.98828125}, {"start": 1174.78, "end": 1175.16, "word": " مثلا", "probability": 0.977294921875}, {"start": 1175.16, "end": 1175.74, "word": " لبلاستيك", "probability": 0.8575846354166666}, {"start": 1175.74, "end": 1176.12, "word": " بحطها", "probability": 0.9847412109375}, {"start": 1176.12, "end": 1176.38, "word": " في", "probability": 0.990234375}, {"start": 1176.38, "end": 1176.58, "word": " ال", "probability": 0.97607421875}, {"start": 1176.58, "end": 1177.4, "word": " autoclave", "probability": 0.694091796875}, {"start": 1177.4, "end": 1177.72, "word": " bag", "probability": 0.9677734375}], "temperature": 1.0}], "language": "ar", "language_probability": 1.0, "duration": 1180.10775, "duration_after_vad": 1164.3831249999996}
\ No newline at end of file
diff --git a/PL9fwy3NUQKwZWgAb_V1ATlRRsH-6i3cPC/wyOj5_y9CBQ_raw.srt b/PL9fwy3NUQKwZWgAb_V1ATlRRsH-6i3cPC/wyOj5_y9CBQ_raw.srt
new file mode 100644
index 0000000000000000000000000000000000000000..706ffcd38a80c1c48a7d9731aa725acadb3017a8
--- /dev/null
+++ b/PL9fwy3NUQKwZWgAb_V1ATlRRsH-6i3cPC/wyOj5_y9CBQ_raw.srt
@@ -0,0 +1,1064 @@
+1
+00:00:00,880 --> 00:00:04,040
+بدنا نحكي في هذه الـ slide عن الـ safety rules عن
+
+2
+00:00:04,040 --> 00:00:08,300
+قواعد وإجراءات السلامة والوقاية داخل معمل الـ
+
+3
+00:00:08,300 --> 00:00:11,440
+microbiology in the microbiology laboratory
+
+4
+00:00:11,440 --> 00:00:14,480
+infectious materials are processed as the money of
+
+5
+00:00:14,480 --> 00:00:17,480
+the microorganism used in this course may be
+
+6
+00:00:17,480 --> 00:00:20,960
+pathogenic for a human في معمل ال microbiology
+
+7
+00:00:20,960 --> 00:00:25,480
+بدنا نتعامل مع أي إشي كأنه infectious كأنه معدي
+
+8
+00:00:25,480 --> 00:00:29,300
+وممكن يسببلي مرض كأنه pathogenic for a human ممكن
+
+9
+00:00:29,300 --> 00:00:33,600
+أنهلسبب مرض لأ الإنسان احنا هنتعامل مع بكتيريا
+
+10
+00:00:33,600 --> 00:00:37,660
+هنصبخ بكتيريا هنجري فحوصات على بكتيريا هتزرعي
+
+11
+00:00:37,660 --> 00:00:42,420
+بكتيريا هتمسكي طبق في مزروع عليه بكتيريا عشان هيك
+
+12
+00:00:42,420 --> 00:00:46,900
+أي طبق احنا بنعطيكيه اعتبريه انه infectious انه
+
+13
+00:00:46,900 --> 00:00:51,360
+معدي وممكن يسبب اليك مرض also the materials found
+
+14
+00:00:51,940 --> 00:00:55,740
+برضه الأدوات الموجودة في المعمل such as glass
+
+15
+00:00:55,740 --> 00:00:59,340
+equipment زي زجاجيات من baker, flask, cylinder,
+
+16
+00:00:59,500 --> 00:01:03,460
+tube, biological agent and chemical المواد
+
+17
+00:01:03,460 --> 00:01:06,500
+الكيميائية برضه بدي أتعامل معها كإنها infectious
+
+18
+00:01:06,500 --> 00:01:10,920
+كإنها معضية وممكن إنها تسببلي مرض Campos safety
+
+19
+00:01:10,920 --> 00:01:15,640
+hazard ممكن إنها تشكل خطر على حياتناIf you don't
+
+20
+00:01:15,640 --> 00:01:18,960
+follow laboratory protocol إذا ما اتبعنا قواعد
+
+21
+00:01:18,960 --> 00:01:24,940
+السلامة وامان داخل معمل الـ microbiology As a
+
+22
+00:01:24,940 --> 00:01:28,920
+result لذا قالك certain rules هناك قواعد are
+
+23
+00:01:28,920 --> 00:01:31,680
+necessary to prevent the spread of infectious
+
+24
+00:01:31,680 --> 00:01:37,340
+agent ضروري أن نلتزم فيها حتى نمنع انتشار العدوى
+
+25
+00:01:37,340 --> 00:01:41,500
+The possibility of infection yourselves or other
+
+26
+00:01:41,500 --> 00:01:46,540
+peopleوحتى أقلل فرصة احتمالية اصابة نفسي او حد
+
+27
+00:01:46,540 --> 00:01:50,440
+قريب مني بالعدوى كيف ممكن حد قريب مني بالعدوى
+
+28
+00:01:50,440 --> 00:01:54,860
+ينصاب روحتي انت على سبيل المثال زراعتي بكتيريا بعد
+
+29
+00:01:54,860 --> 00:01:59,460
+ما زراعتي حطيت ايدك على ال bench طبعا هيك ال bench
+
+30
+00:01:59,460 --> 00:02:03,920
+بيصير ملوث بالبكتيريا اجت صحابيتك حطت قلم على ال
+
+31
+00:02:03,920 --> 00:02:08,060
+benchخلص المعمل أخدت القلم و حطته بالشنطة بعد ما
+
+32
+00:02:08,060 --> 00:02:11,840
+روحت على البيت اجى أخوها الصغير و حط هذا القلم في
+
+33
+00:02:11,840 --> 00:02:17,120
+تمه هان الأخوها الصغير اتعرض لعدوى نتيجة عدم
+
+34
+00:02:17,120 --> 00:02:20,540
+اتباعها إجراءات السلامة و البقايا في معمل الـ
+
+35
+00:02:20,540 --> 00:02:24,900
+Microbiology عشان هيك لازم نتبع هذه الإجراءات حتى
+
+36
+00:02:24,900 --> 00:02:31,630
+نحمي نفسنا و نحمي كل قريبنا من العدوىبرضه الهاتف
+
+37
+00:02:31,630 --> 00:02:35,250
+ممنوع نمسكه في معمل الـ Microbiology أي أدوات خلال
+
+38
+00:02:35,250 --> 00:02:39,430
+الشغل أنا بدي استخدمها ضروري بعد ما أنا قبل ما
+
+39
+00:02:39,430 --> 00:02:46,810
+أطلع من معمل الـ Microbiology إن أنا انعقبهم All
+
+40
+00:02:46,810 --> 00:02:50,070
+healthcare workers should routinely use
+
+41
+00:02:50,070 --> 00:02:54,410
+appropriate barrier evacuation قلنا كل العاملين في
+
+42
+00:02:54,410 --> 00:03:01,130
+المجال الصحي لازم يستخدموا بشكل روتينيprecuation
+
+43
+00:03:01,130 --> 00:03:05,170
+يعني وسائل حماية مناسبة لازم يستخدموا وسائل حماية
+
+44
+00:03:05,170 --> 00:03:10,770
+مناسبة to prevent skin حتى يحموا جلدهم add mucus
+
+45
+00:03:10,770 --> 00:03:16,130
+membrane الأخشية المخاطية exposure من أنه تتعرض
+
+46
+00:03:16,130 --> 00:03:20,490
+when contact with blood or other body fluid of any
+
+47
+00:03:20,490 --> 00:03:25,230
+patient حتى أنه تتعرض لهذا ال pathogen أو
+
+48
+00:03:25,230 --> 00:03:29,490
+البكتيريا من العينات المختلفة سواء من blood أو من
+
+49
+00:03:29,720 --> 00:03:33,800
+سوائل الجسم المختلفة طيب كيف العاملين في المجال
+
+50
+00:03:33,800 --> 00:03:37,880
+الصحي بيحموا جلدهم بيلبسوا من وسائل الحماية أنهم
+
+51
+00:03:37,880 --> 00:03:41,700
+يلبسوا ل gloves and mucus membrane كيف بدي أحمي
+
+52
+00:03:41,700 --> 00:03:46,240
+الأغشية المخاطية أني أنا ألبس ال mask تمام بالنسبة
+
+53
+00:03:46,240 --> 00:03:50,100
+لقواعة السلامة أهم إشي أني أنا أبدأ بحماية نفسي
+
+54
+00:03:50,100 --> 00:03:55,600
+لأنه لو حاميت نفسي أكيد هحمي الآخرين من العدوى أول
+
+55
+00:03:55,600 --> 00:04:00,780
+نقطة هي للناس اللي برا لأنهاحنا محجبين للناس اللي
+
+56
+00:04:00,780 --> 00:04:05,400
+بشعرهم بقولك لازم انك انت اتحافظي على شعرك يكون
+
+57
+00:04:05,400 --> 00:04:10,880
+مربوط لأنه ممكن خلال شغلك اذا ال كان مش مربوط
+
+58
+00:04:10,880 --> 00:04:15,500
+الشعر ينزل على الطبق البكتيري always wearing
+
+59
+00:04:15,500 --> 00:04:20,980
+closed shoes في معمل ال microbiology لازم ال shoes
+
+60
+00:04:20,980 --> 00:04:25,320
+يكون closed ممنوع نلبس صنادر لأنه ممكن يكون في
+
+61
+00:04:25,320 --> 00:04:32,090
+رجلك يكون في جلحروحتي انت زراعتي بكتيريا طبعا خلال
+
+62
+00:04:32,090 --> 00:04:35,790
+الزراعة مثلا انا بستخدم فيه أداء اسمه الوب في
+
+63
+00:04:35,790 --> 00:04:40,990
+الزراعة وقعت هذه اللوب على الأرض وإجت على رجلك
+
+64
+00:04:40,990 --> 00:04:44,210
+اللي فيها الجروح طبعا اللوب فيها بكتيريا هذه
+
+65
+00:04:44,210 --> 00:04:48,410
+البكتيريا هتدخل من هذا الجروح للجسم وتعملك
+
+66
+00:04:48,410 --> 00:04:52,210
+systemic infection طبعا هذه systemic infection إيش
+
+67
+00:04:52,210 --> 00:04:55,870
+يعني عدوى بتدخل قدامه وممكن تروح لأى مكان في الجسم
+
+68
+00:04:55,870 --> 00:05:01,420
+وتسببلك أمراضwearing personal protection equipment
+
+69
+00:05:01,420 --> 00:05:06,220
+لازم نلبس اللي هي ال gloves ال lab coat ال eye
+
+70
+00:05:06,220 --> 00:05:11,020
+protection ممنوع أنا أشتغل في معمل ال microbiology
+
+71
+00:05:11,020 --> 00:05:15,220
+أزرع بكتيريا بدون ما ألبس ال coat و ال gloves ما
+
+72
+00:05:15,220 --> 00:05:18,980
+بضمن أنه أي إشي يدخل جسمي و يسببلي infection ال
+
+73
+00:05:18,980 --> 00:05:22,480
+eye protection اللي هي ال googles أنا بعض الأحيان
+
+74
+00:05:22,480 --> 00:05:25,880
+ممكن أستخدم ال eye protection لالتغطية لأن ممكن
+
+75
+00:05:25,880 --> 00:05:29,810
+نزرع بكتيريا سائلةأتكلمنا عن الأوساط الغذائية في
+
+76
+00:05:29,810 --> 00:05:33,710
+المحاضرة التعليفية في بعض أنواع ال media ممكن أنها
+
+77
+00:05:33,710 --> 00:05:36,870
+تكون الأوساط الغذائية تكون سائلة روحت أنت زرعت
+
+78
+00:05:36,870 --> 00:05:40,670
+بكتيريا ممكن تيجي على عينك فبالتالي ال googles
+
+79
+00:05:40,670 --> 00:05:44,810
+اللي هي ال eye protection هان مهمة حتى أمنع أنها
+
+80
+00:05:44,810 --> 00:05:48,750
+تيجي على عيني و تسببلي عدوى never applying
+
+81
+00:05:48,750 --> 00:05:53,280
+cosmetics ممنوع أني أناطبعا أحط أدوات تجميل or
+
+82
+00:05:53,280 --> 00:05:56,480
+blessing object finger pencil in the mouth or
+
+83
+00:05:56,480 --> 00:06:01,200
+touching the face ممنوع تمسك أصابعك أو القلم طبعا
+
+84
+00:06:01,200 --> 00:06:05,960
+سواء في طمك أو أنه يصير touch في الوجه في كتير
+
+85
+00:06:05,960 --> 00:06:10,640
+بنات مثلا بتروح تزرع بكتيريا بعد ما تزرع بتهتستنى
+
+86
+00:06:10,640 --> 00:06:14,500
+صحبيتها قبل ما تخلص بتضلها في ال .. طبعا في ال ..
+
+87
+00:06:14,500 --> 00:06:18,950
+في ال groupبعدها تقعد تستنى بتروح حاطة اللى هى
+
+88
+00:06:18,950 --> 00:06:22,730
+إيديها على وجهها بالتالي يصير في عندي touch بين ال
+
+89
+00:06:22,730 --> 00:06:27,970
+.. بين البكتيريا و الوجه ممكن يسببلها عدوى و مشاكل
+
+90
+00:06:27,970 --> 00:06:32,490
+في الوجهة never eating, drinking or chewing gum in
+
+91
+00:06:32,490 --> 00:06:35,530
+the laboratory ممنوع الأكل و الشراب لأنه ممكن
+
+92
+00:06:35,530 --> 00:06:42,470
+يتلوث الطعام و الشراب بهذه البكتيريا بالنسبة
+
+93
+00:06:42,470 --> 00:06:47,300
+لمنطقة الشغالfor working washing hand period and
+
+94
+00:06:47,300 --> 00:06:51,120
+after lab working with water and soap ضروري بدنا
+
+95
+00:06:51,120 --> 00:06:56,000
+نغسل إيدينا قبل و بعد الشغل بالمية والصابون keep
+
+96
+00:06:56,000 --> 00:07:00,280
+the lab bench free of unnecessary material ضروري
+
+97
+00:07:00,280 --> 00:07:04,600
+المكان الشغل أو منطقة الشغل فقط الأشياء الضرورية
+
+98
+00:07:04,600 --> 00:07:07,580
+اللي أنا بحتاجها خلال شغلي انها تكون الأشياء الغير
+
+99
+00:07:07,580 --> 00:07:12,340
+ضروريةطبعا ممنوع أنها نحطها حتى لأنه ممكن أنها
+
+100
+00:07:12,340 --> 00:07:17,160
+تكون مصدر للعدوى فيما بعد disinfecting lab bench
+
+101
+00:07:17,160 --> 00:07:21,440
+period to end at the conclusion of each lab
+
+102
+00:07:21,440 --> 00:07:26,420
+session بقولك ها disinfecting هاي معناها أننا بدنا
+
+103
+00:07:26,420 --> 00:07:32,200
+نطهر ال bench ما قبل ما أبدأ شغلي حتى أني أنا أغمن
+
+104
+00:07:32,200 --> 00:07:35,960
+أنه مافي أي إشي على ال bench في هذه المنطقة أي
+
+105
+00:07:35,960 --> 00:07:41,960
+بكتيريا أي contaminationوكمان بعد ما انت من شغلي
+
+106
+00:07:41,960 --> 00:07:47,480
+في المعمل ضروري انه ان احنا نعقم المكان وينطهره
+
+107
+00:07:47,480 --> 00:07:51,740
+يبقى في عندي انا مصطلحين disinfection وفي عندي
+
+108
+00:07:51,740 --> 00:07:56,360
+مصطلح تاني sterilization هدول المصطلحين بشكل مختصر
+
+109
+00:07:56,360 --> 00:08:01,620
+الفرق بينهم ال disinfection معناه تطهير بدي اقتل
+
+110
+00:08:01,620 --> 00:08:05,770
+فقط ال pathogenic bacteriaالـ disinfection معناها
+
+111
+00:08:05,770 --> 00:08:10,330
+تطهير كل pathogenic بكتيريا فقط بدي أقتل البكتيريا
+
+112
+00:08:10,330 --> 00:08:14,630
+اللي بتسببلي أمراض بالنسبة لـ sterilization اللي
+
+113
+00:08:14,630 --> 00:08:20,990
+هي التعقيم التعقيم انه kill all microorganism or
+
+114
+00:08:20,990 --> 00:08:25,330
+kill all بكتيريا بدي أقتل كل البكتيريا في ان احنا
+
+115
+00:08:25,330 --> 00:08:30,840
+جهاز بنستخدمه لـ sterilization هنتحدث فيهفي الجزء
+
+116
+00:08:30,840 --> 00:08:34,500
+الأخير من المعمل هذا الجهاز اسمه الـ Autoclave
+
+117
+00:08:34,500 --> 00:08:38,880
+مندخل كل الأدوات اللي أنا بدي أشتغل فيها من Loop
+
+118
+00:08:38,880 --> 00:08:45,120
+من Tube وغيرها من الأمور اللي أنا بستخدمها في شغلي
+
+119
+00:08:45,120 --> 00:08:48,480
+وبدي أدخلها في جهاز الـ Autoclave جهاز الـ
+
+120
+00:08:48,480 --> 00:08:52,620
+Autoclave ضغط عالي ودرجة حرارة عالية بيقتل كل
+
+121
+00:08:52,620 --> 00:08:56,200
+البكتيريا اللي موجودة في الأدوات وهكذا بوفر لي
+
+122
+00:08:56,200 --> 00:09:01,520
+ظروف معقمةGood lab practice بقول لها لو أنا مارست
+
+123
+00:09:01,520 --> 00:09:05,900
+عملي بشكل جيد و لو اتبعت كل إجراءات الوقاية
+
+124
+00:09:05,900 --> 00:09:10,220
+والسلامة including method for aseptic transfer
+
+125
+00:09:10,220 --> 00:09:16,120
+returning materials to proper location proper care
+
+126
+00:09:16,120 --> 00:09:19,840
+and handling of equipment نقلت المواد للمكان
+
+127
+00:09:19,840 --> 00:09:24,450
+الصحيح بالطريقة الصحيحة بالطريقة ال standardكل هذه
+
+128
+00:09:24,450 --> 00:09:28,090
+الأمور لو أنا اتبعتها هحمي حالي من اي infectious
+
+129
+00:09:28,090 --> 00:09:33,350
+من اي عدو من اي infection keeping the bench top
+
+130
+00:09:33,350 --> 00:09:37,830
+clear of extraneous material التكلمنا فيها ان انا
+
+131
+00:09:37,830 --> 00:09:42,760
+لازم احافظ على bench top يعني سطح ال benchلازم
+
+132
+00:09:42,760 --> 00:09:47,280
+أحافظ عليه من أي أمور أنا ما بدي أحتاجها أي أمور
+
+133
+00:09:47,280 --> 00:09:50,680
+unnecessary لازم أني أنا أتخلص منها فقط الأمور
+
+134
+00:09:50,680 --> 00:09:53,720
+الضرورية اللي بحتاجها في شغلي لازم تكون على ال
+
+135
+00:09:53,720 --> 00:09:58,660
+«بنش» هلأ في كل group هيكون طبعاً هيكون في عنده
+
+136
+00:09:58,660 --> 00:10:05,880
+لهب «بنسن» هذا اللهب هنوزع البنات جروبات لما أنا
+
+137
+00:10:05,880 --> 00:10:09,880
+أبدأ أشتغل أول ما أبدأ أشتغل لما نخلص الجزء النظر
+
+138
+00:10:09,880 --> 00:10:14,400
+ونبدأ في الجزء العمليبدنا نغسل أيدانا فيه الميه
+
+139
+00:10:14,400 --> 00:10:20,320
+والصابون و نشفهم منيح بعد كده باجي لمنطقة ال bench
+
+140
+00:10:20,320 --> 00:10:25,240
+كل group بيروح لمنطقة الشغل الخاصة فيه و بيجيب
+
+141
+00:10:25,240 --> 00:10:30,000
+detail بال tissue و بيطهر المكان بعد ما أخلص بال
+
+142
+00:10:30,000 --> 00:10:35,060
+detail بدي أعقم بالكحول بجيب tissue و كحول و بعقم
+
+143
+00:10:35,060 --> 00:10:39,610
+ال benchبعد كده بدي أبدأ الشغل بشغل اللهب بعد ما
+
+144
+00:10:39,610 --> 00:10:45,450
+أخلص الشغل بسكر اللهب و بعدين بجيب دي طول كحول و
+
+145
+00:10:45,450 --> 00:10:52,930
+برش ممنوع أنا يكون اللهب شغال و أروح أجي أجي أرش
+
+146
+00:10:52,930 --> 00:10:59,250
+الكحول و أعقم المكان ليش؟ لأن وقتها هيصير عندى
+
+147
+00:10:59,250 --> 00:11:03,590
+طبعا انفجار في المكان ممنوع الكحول و اللهب يشتموا
+
+148
+00:11:04,690 --> 00:11:08,070
+هذا بالنسبة لمنطقة الشغل شو الإجراءات اللي احنا
+
+149
+00:11:08,070 --> 00:11:14,710
+بنعملها ما قبل و بعد شغلي dispose
+
+150
+00:11:14,710 --> 00:11:19,250
+all contaminated material in autoclave bag and
+
+151
+00:11:19,250 --> 00:11:22,530
+prepare disposable of other different type of pest
+
+152
+00:11:23,200 --> 00:11:27,660
+الـ despose يعني بدي أتخلص من all كونتا منادت
+
+153
+00:11:27,660 --> 00:11:31,420
+كونتا منادت material يعني مواد ملوثة بدي أتخلص من
+
+154
+00:11:31,420 --> 00:11:35,460
+كل المواد الملوثة اللي أنا استخدمتها في شغل من بيت
+
+155
+00:11:35,460 --> 00:11:38,880
+الرفاش من اللوب كل هذه الأدوات بعد ما أخلص تعتبر
+
+156
+00:11:38,880 --> 00:11:43,480
+كونتا مناشن تعتبر ملوثات كونتا منادت فيها بكتيريا
+
+157
+00:11:43,480 --> 00:11:48,220
+فبالتالي لازم أحطها في إشي اسمه autoclave bag ال
+
+158
+00:11:48,220 --> 00:11:52,970
+autoclave bag هي عبارة عن كيسبعد ما أخلص الشغل بحط
+
+159
+00:11:52,970 --> 00:11:56,230
+الأطباق اللي بتريدش اللي زراعتها و بحطها في هذا ال
+
+160
+00:11:56,230 --> 00:11:59,670
+case ليش بحطها في هذا ال case؟ عشان يدخلوها في
+
+161
+00:11:59,670 --> 00:12:04,350
+جهاز ال autoclave عشان نعقمها و هيك نحمي حتى عمال
+
+162
+00:12:04,350 --> 00:12:08,610
+مثلاين نظافة من ال infection the code should be
+
+163
+00:12:08,610 --> 00:12:13,280
+kept separately from other things in your bagوقولك
+
+164
+00:12:13,280 --> 00:12:16,420
+الـ code لازم احنا نجيب كيس معمل الـ microbiology
+
+165
+00:12:16,420 --> 00:12:20,300
+وكيس خاص ونحطه فيه ممنوع امسك ال lab code و احطه
+
+166
+00:12:20,300 --> 00:12:24,020
+في الشنطة لأن خلال شغلك ما بتضمني ان يكون بكتيريا
+
+167
+00:12:24,020 --> 00:12:27,400
+اجت على ال lab code روح تحطيها ال lab code في شنطك
+
+168
+00:12:27,400 --> 00:12:32,160
+بالتالي هي كل الأدوات في الشنطة هتصير ملوثة
+
+169
+00:12:32,160 --> 00:12:36,220
+بالبكتيريا وممكن انها تسبب infection لإلك
+
+170
+00:12:36,220 --> 00:12:42,700
+Departing all injury accidents spells بقولنالازم
+
+171
+00:12:42,700 --> 00:12:48,600
+احنا كل الحوادث كل ال spells اش يعني ال spells؟ ال
+
+172
+00:12:48,600 --> 00:12:54,820
+spells يعني الانسكابات and broken ال class where
+
+173
+00:12:54,820 --> 00:13:00,120
+الأشياء المكسورة ضروري جدا انه طبعا ينبه ال
+
+174
+00:13:00,120 --> 00:13:03,640
+instructor ال instructor يعني اللي بيعطي التعليمات
+
+175
+00:13:03,640 --> 00:13:08,780
+and receiving instruction for clean up ونستقبل منه
+
+176
+00:13:08,780 --> 00:13:15,590
+التعليمات كيف انه احنا ممكنان انا بحاول ان اتخلص
+
+177
+00:13:15,590 --> 00:13:19,890
+من هذا ال spells or broken glassware طبعا هنتعرف
+
+178
+00:13:19,890 --> 00:13:23,290
+كمان شوية في كمان slide تان على كيف انا ممكن لو
+
+179
+00:13:23,290 --> 00:13:26,710
+صار في عندي spells لو صار في عندي انسكاب او اشي
+
+180
+00:13:26,710 --> 00:13:31,300
+انكسر ججاجي كيف ان انا اتعامل معاهalways using
+
+181
+00:13:31,300 --> 00:13:34,940
+appropriate bipeding device and understand that
+
+182
+00:13:34,940 --> 00:13:38,440
+mouth bipeding is forbidden عارفين أنتم ال mouth
+
+183
+00:13:38,440 --> 00:13:42,940
+bipeding أكيد مرهت معاكم هاي ممنوع forbidden ممنوع
+
+184
+00:13:42,940 --> 00:13:47,220
+أني أنا أستعملها في معمل ال microbiology لأنه ممكن
+
+185
+00:13:47,220 --> 00:13:50,860
+يكون في عندي بكتيريا فبالتالي أنا أروح أشفط
+
+186
+00:13:50,860 --> 00:13:55,130
+بكتيريا و هيك تسببلي عدوىUsing universal pre
+
+187
+00:13:55,130 --> 00:13:58,670
+-equation post in the lab and seen inside front
+
+188
+00:13:58,670 --> 00:14:03,290
+cover of this laboratory manual Universal يعني
+
+189
+00:14:03,290 --> 00:14:07,370
+عالمي، pre-equation قلنا وسائل حماية في عندي أنا
+
+190
+00:14:07,370 --> 00:14:12,910
+في كل قسم مثلا أو في كل معمل في عندي lab manual أو
+
+191
+00:14:13,860 --> 00:14:19,180
+الكتاب خاص هذا الكتاب بيكون فيه كل فحص كل الفحصات
+
+192
+00:14:19,180 --> 00:14:22,160
+اللي بنعملها في القسم الـ Microbiology اسم هذا
+
+193
+00:14:22,160 --> 00:14:25,520
+الفحص، أشهر الأدوات اللي أنا بستخدمها، شو إجراءات
+
+194
+00:14:25,520 --> 00:14:30,440
+السلامة و الوقاية، و اللي كان لازم انت طبعا تشوفي
+
+195
+00:14:30,440 --> 00:14:33,280
+لو انت دخلت القسم ال Microbiology انك انت تشوفي
+
+196
+00:14:33,280 --> 00:14:40,580
+هذا ال lab manual Safety
+
+197
+00:14:40,580 --> 00:14:45,390
+for dealing with specimenقواعد السلامة والأمان في
+
+198
+00:14:45,390 --> 00:14:49,790
+تعاملنا مع العينة blood and other body fluid from
+
+199
+00:14:49,790 --> 00:14:53,130
+all patients should be considered infective أحنا
+
+200
+00:14:53,130 --> 00:14:57,030
+قلنا أي إشي في معمل الـ microbiology لازم نعتبر أن
+
+201
+00:14:57,030 --> 00:15:00,450
+هو infectious فمبالك في العينات اللي بتيجي من
+
+202
+00:15:00,450 --> 00:15:04,130
+المرضى عشان أنا بدي أفحصها برضه لازم أني أنا
+
+203
+00:15:04,130 --> 00:15:09,510
+أعتبرها أنها معدية ممكن تنقلي العدوىAll healthcare
+
+204
+00:15:09,510 --> 00:15:13,650
+workers should take precaution to prevent injury
+
+205
+00:15:13,650 --> 00:15:18,490
+caused by needle واللي كان كل العاملين في المجال
+
+206
+00:15:18,490 --> 00:15:23,350
+الصحي لازم ياخدوا بالهم ويحموا أنفسهم من ال injury
+
+207
+00:15:23,350 --> 00:15:28,140
+اللي بتسببها من الإصابات الممكن يسببها by needleor
+
+208
+00:15:28,140 --> 00:15:32,000
+scalpels ايش يعني scalpels يعني مش روت and other
+
+209
+00:15:32,000 --> 00:15:35,520
+sharp instrument او الأدوات الحادة or devised
+
+210
+00:15:35,520 --> 00:15:39,780
+during a procedure خلال شغلي انا لازم اخد بالي لو
+
+211
+00:15:39,780 --> 00:15:43,880
+انا استخدمت needle او أدوات حادة حتى اني انا ما
+
+212
+00:15:43,880 --> 00:15:50,900
+طبعا ممكن ال needle انها يسببطبعا تنغز في ال
+
+213
+00:15:50,900 --> 00:15:54,880
+needle و تسببلي عدوى بعض الأحيان ال needle أنا
+
+214
+00:15:54,880 --> 00:15:58,680
+بستخدمها في فحص المضادات الحيوية بروح بزرع بكتيريا
+
+215
+00:15:58,680 --> 00:16:02,000
+في طباق و بعدين بجبدي أحط أقراس ال antibiotic على
+
+216
+00:16:02,000 --> 00:16:06,100
+هذه البكتيريا فبستخدم ال needle في وضع ال
+
+217
+00:16:06,100 --> 00:16:10,200
+antibiotic على البكتيريا فهك ممكن يصير touch بين
+
+218
+00:16:10,200 --> 00:16:14,390
+ال needle و بين البكتيرياوممكن أنا نتيجة طبعا
+
+219
+00:16:14,390 --> 00:16:18,530
+استخدامي اني انا ما اتخدت اجراءاتي الوقاية ممكن
+
+220
+00:16:18,530 --> 00:16:22,470
+انا انغز بالإبرة وهيك طبعا الإبرة فيها بكتيريا و
+
+221
+00:16:22,470 --> 00:16:26,970
+تدخل الرسم وتسببلي infection هان بقولي لازم اني
+
+222
+00:16:26,970 --> 00:16:31,040
+انا ااخد كل الإجراءات حتى امنعالحوادث اللي ممكن
+
+223
+00:16:31,040 --> 00:16:35,660
+اتسببها ايه الأدوات الحادة to prevent needle stick
+
+224
+00:16:35,660 --> 00:16:39,400
+injuries needle shouldn't be recapped ممنوع انا
+
+225
+00:16:39,400 --> 00:16:43,080
+حتى اني انا احمل حالي من غزة الإبرة ممنوع اني ارجع
+
+226
+00:16:43,080 --> 00:16:47,740
+احط الغطاية فتحت ال needle ممنوع اقلط اني ارجع
+
+227
+00:16:47,740 --> 00:16:54,640
+اغطيها بيه اللي هي غطيتهاavoid contaminating the
+
+228
+00:16:54,640 --> 00:16:58,180
+outside of the container container طبعا ال safety
+
+229
+00:16:58,180 --> 00:17:01,680
+box هذا اللي عارفينه ال safety box بنعرف لازم ان
+
+230
+00:17:01,680 --> 00:17:05,580
+احنا ناخد بالنا انه ما يتلوث من برا ببكتيريا على
+
+231
+00:17:05,580 --> 00:17:09,400
+سبيل المثال روحت انت زراعتي بكتيريا بال loop اللوب
+
+232
+00:17:09,400 --> 00:17:12,960
+بظل فيها بكتيريا بالغلط صار عندي touch بال
+
+233
+00:17:12,960 --> 00:17:17,600
+container من برا كيك صار ال container ملوث ممكن
+
+234
+00:17:17,600 --> 00:17:23,560
+تيجي أزميلتك يتفكروا انه نظيف يصير عندي عدوىويسبب
+
+235
+00:17:23,560 --> 00:17:28,040
+لها مرض عشان هيك من أول ما اتكلمنا عن الـ Safety
+
+236
+00:17:28,040 --> 00:17:31,280
+Rules قلنا لازم نتعامل مع أي إشي في معمل الـ
+
+237
+00:17:31,280 --> 00:17:35,620
+Microbiology كإنه infectious كإنه معدي All
+
+238
+00:17:35,620 --> 00:17:39,000
+specimens should be bought in a well-constructed
+
+239
+00:17:39,000 --> 00:17:42,280
+container with a secure lid to prevent leaking
+
+240
+00:17:42,280 --> 00:17:46,840
+during transport قلنا كل العينات لازم أني أنا
+
+241
+00:17:46,840 --> 00:17:52,760
+أحطها في وعاء محكم الإغلاق with a secure lidوكمان
+
+242
+00:17:52,760 --> 00:17:58,300
+غطاء آمن ليش؟ حتى أمنع تسرب العينة أثناء عملية
+
+243
+00:17:58,300 --> 00:18:02,200
+النقل ممكن لو كانت العينة سائلة وكان الغطاء مش
+
+244
+00:18:02,200 --> 00:18:05,460
+محكم و أنا روحت نقلت العينة خلال نقل العينة ممكن
+
+245
+00:18:05,460 --> 00:18:11,660
+هي العينة تسير في عندي مثلا تسريب لمناطق أنا مش
+
+246
+00:18:11,660 --> 00:18:15,820
+ماخدة بالي منها و العينة ممكن يكون فيها طبعا
+
+247
+00:18:15,820 --> 00:18:22,200
+بكتيريا و تسببلي عدوى لناس طبعاخارج حتى المجال
+
+248
+00:18:22,200 --> 00:18:26,060
+الصحي عشان هيك لازم ناخد بالنا إنه العينات كلها
+
+249
+00:18:26,060 --> 00:18:30,840
+نحطها في well-constructed container with a secure
+
+250
+00:18:30,840 --> 00:18:35,780
+lip after they are used disposable syringe and
+
+251
+00:18:35,780 --> 00:18:39,320
+needle scalpel blades and other sharp should be
+
+252
+00:18:39,320 --> 00:18:42,560
+placed in a puncture resistant container for
+
+253
+00:18:42,560 --> 00:18:47,820
+disposable احنا قلنا في ال .. أول ما تكلمنا عن ال
+
+254
+00:18:47,820 --> 00:18:51,840
+safety rulesالأدوات اللي بعد ما انا أخلص بحطها في
+
+255
+00:18:51,840 --> 00:18:55,040
+Auto-Clap Bag الـ Auto-Clap Bag هو عبارة عن كيس
+
+256
+00:18:55,040 --> 00:18:59,520
+هلأ الـ Auto-Clap Bag منحط فيها الأدوات البلاستيك
+
+257
+00:18:59,520 --> 00:19:02,820
+زي الـ Petri dish زي الـ Loops لكن الأدوات الحادة
+
+258
+00:19:02,820 --> 00:19:07,160
+زي الـ Needles، Scalpels، الـ Sharps هاي كلها
+
+259
+00:19:07,160 --> 00:19:10,520
+بحطها ضروري حتى أني انا أتخلص منها بحطها في
+
+260
+00:19:10,520 --> 00:19:15,560
+Puncture-resistant container يعني وعاء مقاوم للخرق
+
+261
+00:19:15,560 --> 00:19:20,340
+لأني انا لو حطيتها للكيسممكن لو حطيت الـ needle في
+
+262
+00:19:20,340 --> 00:19:24,620
+كيس اللي هو الـ autoclave bag ممكن تخرق الكيس وهيك
+
+263
+00:19:24,620 --> 00:19:28,180
+بالتالي ممكن أنه تسبب أداء لإنسان اللي بيحملها
+
+264
+00:19:28,180 --> 00:19:32,120
+عشان هيك الأدوات الحادة أنا بتخلص منها بحطها في
+
+265
+00:19:32,120 --> 00:19:35,160
+puncture resistant container لكن الأدوات مثلا
+
+266
+00:19:35,160 --> 00:19:37,720
+لبلاستيك بحطها في ال autoclave bag
+
diff --git a/PL9fwy3NUQKwZWgAb_V1ATlRRsH-6i3cPC/yt25unVl2bQ_postprocess.srt b/PL9fwy3NUQKwZWgAb_V1ATlRRsH-6i3cPC/yt25unVl2bQ_postprocess.srt
new file mode 100644
index 0000000000000000000000000000000000000000..a15d1784473a9c58acf6c26bdb63e53abbaaac15
--- /dev/null
+++ b/PL9fwy3NUQKwZWgAb_V1ATlRRsH-6i3cPC/yt25unVl2bQ_postprocess.srt
@@ -0,0 +1,2040 @@
+1
+00:00:01,960 --> 00:00:05,660
+السلام عليكم ورحمة الله وبركاته نستكمل معاكم
+
+2
+00:00:05,660 --> 00:00:09,700
+محاضرات مساقى الـ Microbiology العملي ولازلنا في
+
+3
+00:00:09,700 --> 00:00:13,200
+ال biochemical test اليوم موضوعنا هيكون عن
+
+4
+00:00:13,200 --> 00:00:17,240
+bacterial fermentation and methyl red vocaspar
+
+5
+00:00:17,240 --> 00:00:21,440
+هنبدأ بال bacterial fermentation microbial
+
+6
+00:00:21,440 --> 00:00:24,340
+metabolic process are complex but they permit the
+
+7
+00:00:24,340 --> 00:00:27,700
+microbiologist to distinguish among microorganism
+
+8
+00:00:27,700 --> 00:00:32,050
+grown in cultureبقولي العمليات الأيضية اللي بتقوم
+
+9
+00:00:32,050 --> 00:00:36,690
+فيها البكتيريا معقدة لكن بتسمح لأخصائي ال
+
+10
+00:00:36,690 --> 00:00:40,510
+microbiology انه يميز بين الميكروبات اللي بتنمو في
+
+11
+00:00:40,510 --> 00:00:44,470
+المزرعة many clinical pathogen can be identified
+
+12
+00:00:44,470 --> 00:00:47,830
+by manipulating pure culture into media that
+
+13
+00:00:47,830 --> 00:00:52,950
+contain one or more specific biochemicals بقولي ال
+
+14
+00:00:52,950 --> 00:00:57,950
+pathogen ممكن ان انا اقدر اعرفها من خلال اني
+
+15
+00:00:57,950 --> 00:01:03,760
+بزرعهافي pure culture وصلت العينة بروح انا بزراها
+
+16
+00:01:03,760 --> 00:01:08,040
+بطريقة ال quadrant على سبيل المثال كانت عينة تيرين
+
+17
+00:01:08,040 --> 00:01:12,360
+روحت زراعتها بطريقة ال quadrant بحصل على isolated
+
+18
+00:01:12,360 --> 00:01:15,380
+كلني من خلال طريقة ال quadrant من خلال طريقة
+
+19
+00:01:15,380 --> 00:01:19,680
+الأربعة هذه ال isolated كلني باخدها و بعمل pure
+
+20
+00:01:19,680 --> 00:01:22,880
+culture بروح على طبق بزراها هذه ال isolated كلني
+
+21
+00:01:22,880 --> 00:01:27,160
+باخدها و بزراها حتى احصل على pure culture هيك
+
+22
+00:01:27,160 --> 00:01:31,640
+باطمنإن المزرعة هيكون أصلها خلية بكتيريا واحدة
+
+23
+00:01:31,640 --> 00:01:35,620
+يعني أنا لما بدي أشتغل هشتغل على بكتيريا واحدة فقط
+
+24
+00:01:35,620 --> 00:01:39,460
+X مش mixed population عشان تكون نتائجي ال
+
+25
+00:01:39,460 --> 00:01:43,920
+biochemical test صح يبقى ال pure culture مهمة جدا
+
+26
+00:01:43,920 --> 00:01:51,000
+عشان أشتغل الفحوصات الكيميائية أنا لما أزرعالعينة
+
+27
+00:01:51,000 --> 00:01:54,520
+و احصل على isolator كله اني بعدين اعمل pure
+
+28
+00:01:54,520 --> 00:01:58,400
+culture باخد من ال pure culture و بشتغل فحوصات
+
+29
+00:01:58,400 --> 00:02:04,140
+كيميائية الفحص الكيميائي ممكن يكون عبارة عن media
+
+30
+00:02:04,140 --> 00:02:07,520
+بروح بزراحي ال pure culture في media بتحتوي على
+
+31
+00:02:07,520 --> 00:02:11,840
+one specific biochemical شو قصده بهذه الجملة على
+
+32
+00:02:11,840 --> 00:02:16,860
+سبيل المثال فحص السيمون سايتراتأو فحص السيطرات أنا
+
+33
+00:02:16,860 --> 00:02:21,140
+بستخدم media simon citrate agar media اسمها simon
+
+34
+00:02:21,140 --> 00:02:26,880
+citrate agar هذه ال media فقط بتكشفلي على فحص
+
+35
+00:02:26,880 --> 00:02:30,000
+كيميائي واحد اللي هو السيطرات هل البكتيريا عندها
+
+36
+00:02:30,000 --> 00:02:35,240
+القدرة على استهلاك السيطرات أو لأ لكن في بعض أنواع
+
+37
+00:02:35,240 --> 00:02:39,240
+ال media لما أنا أزرعها أزرع البكتيريا من ال bio
+
+38
+00:02:39,240 --> 00:02:42,680
+culture في هذه ال media بتكشفلي more specific
+
+39
+00:02:42,680 --> 00:02:46,750
+biochemical أكتر من فحص كيميائيهنتعرف في chapter
+
+40
+00:02:46,750 --> 00:02:51,830
+الـ Single Media Multiple Test على هذا الموضوع انه
+
+41
+00:02:51,830 --> 00:02:54,470
+ميديا انا بزرع البكتيريا في ميديا هذه الميديا
+
+42
+00:02:54,470 --> 00:03:00,050
+بتكشفلي على اكتر من فحص كيميائي كيف انا بدي استدل
+
+43
+00:03:00,050 --> 00:03:04,330
+انه التفاعل صار او لأ بقولنا من خلال نواتج التفاعل
+
+44
+00:03:04,330 --> 00:03:09,230
+بشوف النتائج ممكن بال next eye زي فحص الكتلاز كنت
+
+45
+00:03:09,230 --> 00:03:14,360
+اشوف ال air bubbles بعيني فحص ال coagulaseبرضه
+
+46
+00:03:14,360 --> 00:03:17,600
+ممكن اني انا اشوفها بالناقد I كنت اشوف الـ Clumpy
+
+47
+00:03:17,600 --> 00:03:24,660
+ممكن برضه اني انا اضيف Reagent في الميديا هذا الـ
+
+48
+00:03:24,660 --> 00:03:31,000
+Reagent يتفاعل مع نواتج التفاعل او يغير من اللغة
+
+49
+00:03:31,000 --> 00:03:34,960
+الميديا على سبيل المثال فحص السيطرات فحص السيطرات
+
+50
+00:03:34,960 --> 00:03:38,960
+كان في NDPH Indicator اللي هو الـ Bromothymol Blue
+
+51
+00:03:38,960 --> 00:03:45,560
+كان موجود من مكونات الميديالما التفاعل لو
+
+52
+00:03:45,560 --> 00:03:48,580
+البكتيريا عندها القدرة على استهلاك السيطرات قولنا
+
+53
+00:03:48,580 --> 00:03:51,860
+هيتكون الـ Climate Product اللي هو الـ Ammonia الـ
+
+54
+00:03:51,860 --> 00:03:56,660
+NH3 هيرفع الـ pH في عندي pH Indicator في الـ Media
+
+55
+00:03:56,660 --> 00:04:02,240
+هيتغير لون الـ Media حسب طبعا شو الـ pH Indicator
+
+56
+00:04:02,240 --> 00:04:06,220
+وشو لونه في ال Acidic أو الBasic حسب نواتج التفاعل
+
+57
+00:04:06,220 --> 00:04:11,060
+كان في السيطرات ناتج التفاعل Ammoniaالـ pH
+
+58
+00:04:11,060 --> 00:04:15,680
+Indicator في الـ Basic كان لونه blue يبقى هيتغير
+
+59
+00:04:15,680 --> 00:04:19,180
+لون ال media من اللون الأخضر لللون الأزرق هيك أنا
+
+60
+00:04:19,180 --> 00:04:24,200
+بعرف أنه البكتيريا قدرت تستهلك السيطرات يبقى هي
+
+61
+00:04:24,200 --> 00:04:30,180
+سيطرات بوزيفة هذا بالنسبة للـ Reagent أو الـ pH
+
+62
+00:04:30,180 --> 00:04:34,460
+Indicator كان موجود في ال media ممكن أنا بعد ال
+
+63
+00:04:34,460 --> 00:04:39,470
+incubationأضيف reagent يتفاعل مع نواتج التفاعل زي
+
+64
+00:04:39,470 --> 00:04:43,250
+فحص الـ amylase الـ amylase كنا نضيف ال iodine بعد
+
+65
+00:04:43,250 --> 00:04:47,250
+ال incubation يبقى نواتج التفاعل ممكن ان انا
+
+66
+00:04:47,250 --> 00:04:51,670
+اشوفها بالـ next I زي فحص الكتلاز والكواجولاز ممكن
+
+67
+00:04:51,670 --> 00:04:55,550
+ان انا اضيف pH indicator او reagent يتفاعل مع
+
+68
+00:04:55,550 --> 00:04:58,510
+نواتج التفاعل هذا reagent يكون موجود في ال media
+
+69
+00:04:58,510 --> 00:05:03,740
+زي فحص السيطرات او ممكن ان انا اضيف reagent بعدالـ
+
+70
+00:05:03,740 --> 00:05:08,300
+Incubation هذا زي فحص الـ amylase هذا الـ Reagent
+
+71
+00:05:08,300 --> 00:05:13,940
+هيتفاعل مع نواتج التفاعل لأنه ممكن يكون الـ
+
+72
+00:05:13,940 --> 00:05:18,280
+Reagent هذا بيتكسر في الـ Autoclave عشان هيك أنا
+
+73
+00:05:18,280 --> 00:05:21,760
+بضيفه بعد الـ Incubation الـ reaction البيو
+
+74
+00:05:21,760 --> 00:05:25,580
+كيميائية التي تأتي بـ Blast Candidatebe determined
+
+75
+00:05:25,580 --> 00:05:28,700
+by relatively simple indicator reagent بقول كيف
+
+76
+00:05:28,700 --> 00:05:32,440
+بستدل على التفاعل من خلال simple indicator reagent
+
+77
+00:05:32,440 --> 00:05:35,520
+هذا ال reagent ممكن يكون موجود في ال medium زي فحص
+
+78
+00:05:35,520 --> 00:05:39,380
+السيطرات أو ممكن أنا أضيفه على المزرعة بعد ال
+
+79
+00:05:39,380 --> 00:05:43,300
+incubation زي فحص ال amylase أو قولنا ممكن أني أنا
+
+80
+00:05:43,300 --> 00:05:48,560
+أشوف التفاعل بال next eye زي فحص الكتلاز و ال
+
+81
+00:05:48,560 --> 00:05:51,600
+coagulase some bacteria ferment carbohydrates
+
+82
+00:05:52,800 --> 00:05:57,520
+producing acidic alcohol or gases in products بقول
+
+83
+00:05:57,520 --> 00:06:00,540
+لي ان بعض البكتيريا عندها القدرة على تخمير
+
+84
+00:06:00,540 --> 00:06:05,880
+السكريات بتنتج acid or alcohol or gas يبقى نواتج
+
+85
+00:06:05,880 --> 00:06:10,880
+عملية ال fermentation acid or alcohol or gas بعض
+
+86
+00:06:10,880 --> 00:06:15,280
+أنواع الميكروبات بتنتج acid with gas وفي بعض الآخر
+
+87
+00:06:15,280 --> 00:06:21,510
+بتنتج ال acid without gasكيف بدنا نميز البكتيريا؟
+
+88
+00:06:21,510 --> 00:06:24,370
+many different species are distinguished on the
+
+89
+00:06:24,370 --> 00:06:28,250
+basis of the carbohydrate they do or don't attack
+
+90
+00:06:28,250 --> 00:06:31,970
+as well as by the nature of end product formed
+
+91
+00:06:31,970 --> 00:06:35,870
+during fermentation بقول لي بقدر أميز بين
+
+92
+00:06:35,870 --> 00:06:41,270
+الميكروبات من خلال أنواع الكربوهيدرات اللي بتستطيع
+
+93
+00:06:41,270 --> 00:06:47,360
+تخميرها يبقى بقدر أميز بين الميكروبات من خلالنوع
+
+94
+00:06:47,360 --> 00:06:52,940
+الكربوهيدرات اللى بتقدر انها اتخمرها كاف يعنى
+
+95
+00:06:52,940 --> 00:06:58,840
+قولنا انه اللى من انواع الكربوهيدرات الجليكوز
+
+96
+00:06:58,840 --> 00:07:02,280
+قولنا في بعض انواع البكتيريا بتقدر تخمر الجليكوز
+
+97
+00:07:02,280 --> 00:07:06,340
+جليكوز fermenter زى الانتيرو بكترياسي في بعض انواع
+
+98
+00:07:06,340 --> 00:07:09,720
+البكتيريا مابتقدر تخمر الجليكوز نون جليكوز
+
+99
+00:07:09,720 --> 00:07:17,070
+fermenter اللى هى الصدومانس من خلالهذا الفحص بقدر
+
+100
+00:07:17,070 --> 00:07:20,870
+ان انا اميز الصدومونس عن الانتيرو بكترياسي قولنا
+
+101
+00:07:20,870 --> 00:07:23,690
+ان الانتيرو بكترياسي بتنقسم ل lactose fermenter و
+
+102
+00:07:23,690 --> 00:07:28,630
+non lactose fermenter يبقى من خلال هذا الفحص بقدر
+
+103
+00:07:28,630 --> 00:07:32,650
+اميز عائلة الانتيرو بكترياسي او ان انا اقسمها
+
+104
+00:07:32,650 --> 00:07:37,280
+لمجموعات يساعدني في التعريفتاني إشي من خلال الـ
+
+105
+00:07:37,280 --> 00:07:40,620
+end product اللى اتكونت خلال عملية ال fermentation
+
+106
+00:07:40,620 --> 00:07:44,380
+قولنا نواتج عملية ال fermentation ممكن تكون acid
+
+107
+00:07:44,380 --> 00:07:49,080
+or alcohol or gas ممكن تكون acid لحاله ممكن تكون
+
+108
+00:07:49,080 --> 00:07:55,220
+acid with gas بكون أنا عارفة البكتيريا X بتعمل أو
+
+109
+00:07:55,220 --> 00:07:59,140
+نواتج عملية ال fermentation acid without gas
+
+110
+00:07:59,140 --> 00:08:04,140
+البكتيريا Y نواتج عملية ال fermentation acid with
+
+111
+00:08:04,140 --> 00:08:08,880
+gasيبقى من خلال نواتج الـ fermentation بقدر أني
+
+112
+00:08:08,880 --> 00:08:13,100
+أنا أتعرف على البكتيريا the nature of a product
+
+113
+00:08:13,100 --> 00:08:17,140
+formed in amino acid metabolism also provide
+
+114
+00:08:17,140 --> 00:08:20,940
+information as to the identification of bacterial
+
+115
+00:08:20,940 --> 00:08:24,500
+species بقولي برضه من خلال ال amino acid
+
+116
+00:08:24,500 --> 00:08:28,540
+metabolism ممكن أنها تقدملي معلومات تساعدني في
+
+117
+00:08:28,540 --> 00:08:33,020
+تعريف ال bacterial speciesعلى سبيل المثال في عندي
+
+118
+00:08:33,020 --> 00:08:37,300
+amino acid اسمه لتريبتوفان في بعض أنواع البكتيريا
+
+119
+00:08:37,300 --> 00:08:42,380
+عندها أنزيم لتريبتوفانياز بتكسر لتريبتوفان تمام
+
+120
+00:08:42,380 --> 00:08:47,560
+وبحصل على نوات اللي هو Indole, Pyrophate و أمونيا
+
+121
+00:08:47,560 --> 00:08:53,440
+الممكن إن أنا أستفيد طبعا من الأمونيا ك نيتروجين
+
+122
+00:08:53,440 --> 00:08:57,660
+سورس البيروفات فيه كمصدر للطاقة ك carbon source
+
+123
+00:08:57,660 --> 00:09:02,860
+هذا من خلال اللي هو in vivoIn vitro ممكن أستغل هذا
+
+124
+00:09:02,860 --> 00:09:07,260
+الانزيم عشان أعمله فحص كيميائي أشوف هل البكتيريا
+
+125
+00:09:07,260 --> 00:09:10,900
+عندها انزيم للـ tryptophanase أو لأ وهذا هيساعدني
+
+126
+00:09:10,900 --> 00:09:16,120
+في ال identification هنتعرف عليه بالتفصيل في ال
+
+127
+00:09:16,120 --> 00:09:20,700
+chapter اللي جاي في فحص الانضال test the
+
+128
+00:09:20,700 --> 00:09:23,740
+production of visible pigment distinguish certain
+
+129
+00:09:23,740 --> 00:09:28,700
+type of bacteria صدمونسي رشد ولبعض أنواع الصبغات
+
+130
+00:09:28,700 --> 00:09:34,740
+تستخدم للتمييزبين أنواع البكتيريا الصودموناس بتفرص
+
+131
+00:09:34,740 --> 00:09:39,440
+صبغة soluble pigment يبقى الصودموناس بتفرص صبغة
+
+132
+00:09:39,440 --> 00:09:52,260
+soluble pigment إيش
+
+133
+00:09:52,260 --> 00:09:57,180
+يعني soluble pigment هي لونها أصفر أو أخضرSoluble
+
+134
+00:09:57,180 --> 00:10:01,660
+pigment يعني بتلون الكلوني و كمان بتنتشر في الطبق
+
+135
+00:10:01,660 --> 00:10:05,800
+يبقى بلاقى الطبق كله الأجار مع الكلوني صار لونها
+
+136
+00:10:05,800 --> 00:10:10,220
+أخضر لكن السيراشيا بقولي insoluble pigment
+
+137
+00:10:30,530 --> 00:10:34,030
+السيراشيا in soluble pigment مايعني in soluble
+
+138
+00:10:34,030 --> 00:10:38,270
+pigment فقط هلاقي لون الكلوني ما هتقدر تنتشر في
+
+139
+00:10:38,270 --> 00:10:42,210
+الطبق لونها بيكون أحمر يبقى Sodomonas soluble
+
+140
+00:10:42,210 --> 00:10:46,310
+pigment مايعني soluble pigment يعني هتلون الكلوني
+
+141
+00:10:46,310 --> 00:10:50,810
+مع الطبق كامل باللون الأخضر أو الأصفر السيراشيا in
+
+142
+00:10:50,810 --> 00:10:55,710
+soluble pigment فقط هتلون الكلوني باللون الأحمرمش
+
+143
+00:10:55,710 --> 00:11:00,870
+الأجار لأنه هي insoluble ما هتقدر تدوب في المزرعة
+
+144
+00:11:00,870 --> 00:11:03,990
+working with a biological structure freshly
+
+145
+00:11:03,990 --> 00:11:08,190
+isolated from clinical specimen the microbiologist
+
+146
+00:11:08,190 --> 00:11:12,430
+uses a carefully selected petri of special media
+
+147
+00:11:12,430 --> 00:11:16,370
+to identify their outstanding biochemical
+
+148
+00:11:16,370 --> 00:11:21,280
+preparators بقوللي إنهخلال شغلي في الـ Biochemical
+
+149
+00:11:21,280 --> 00:11:24,340
+Test بأستخدم الـ Pure Culture وقلنا ليش Pure
+
+150
+00:11:24,340 --> 00:11:27,880
+Culture لأن أصلها خلية بكتيريا واحدة هيك بضمن أنه
+
+151
+00:11:27,880 --> 00:11:32,380
+أنا بعرف بكتيريا واحدة فقط اللي هي X الـ Fresh
+
+152
+00:11:32,380 --> 00:11:37,740
+لازم يكون لأن الـ Enzyme Activity بقل مع مرور
+
+153
+00:11:37,740 --> 00:11:42,180
+الوقت فبعطيني False Result الـ Microbiologist
+
+154
+00:11:42,180 --> 00:11:48,260
+بيقول لي بيختار ال media اللي هيزرع عليها العينات
+
+155
+00:11:48,970 --> 00:11:53,470
+عينايا على أي أساس احنا بنختار ال media بقولنا
+
+156
+00:11:53,470 --> 00:11:58,050
+بنختار ال media حسب نوع العينة ال stool لو وصلتني
+
+157
+00:11:58,050 --> 00:12:02,150
+عينة stool مش نفس ال media اللي هزرع عليها عينة ال
+
+158
+00:12:02,150 --> 00:12:06,270
+urine عشان هيك بدي أجيب media فقط بتنمي ال
+
+159
+00:12:06,270 --> 00:12:09,330
+pathogen ال pathogen الموجود في ال stool بيختلف عن
+
+160
+00:12:09,330 --> 00:12:12,670
+الموجود في ال urine يعني يكون ال common pathogen
+
+161
+00:12:12,670 --> 00:12:16,320
+in stoolيختلف عن الـ Common Pathogen in urine
+
+162
+00:12:16,320 --> 00:12:20,600
+فبالتالي أنا فقط بستخدم ميديا بتنمي الـ Pathogen
+
+163
+00:12:20,600 --> 00:12:27,340
+فبختار ميديا حسب نوع العينة هتتعرفوا في المعمل الـ
+
+164
+00:12:27,340 --> 00:12:31,960
+Diagnostic في الفصل الجاي كل عينة هيكون لها
+
+165
+00:12:31,960 --> 00:12:36,500
+chapter خاص كيف أنا أو شو الميديا اللي هزرع عليها
+
+166
+00:12:36,500 --> 00:12:37,720
+هذه العينة
+
+167
+00:12:46,580 --> 00:12:51,520
+Bacteria mainly produce ATP by بقولّي أن البكتيريا
+
+168
+00:12:51,520 --> 00:12:56,060
+بتنتج الـATP بتلت عملية أول عملية الـAerobic
+
+169
+00:12:56,060 --> 00:13:00,200
+Respiration تانية عملية الـfermentation تالت عملية
+
+170
+00:13:00,200 --> 00:13:03,560
+الـAnaerobic Respiration هنتعرف على كل عملية
+
+171
+00:13:03,560 --> 00:13:09,600
+بالتفصيل الـAerobic Respiration تنفس الهوائي is an
+
+172
+00:13:09,600 --> 00:13:13,500
+oxidative process which uses oxygen as final
+
+173
+00:13:13,500 --> 00:13:17,960
+electron acceptorبقول للتنفس الهوائي البكتيريا
+
+174
+00:13:17,960 --> 00:13:21,960
+اللى بتعمله لازم تكون obligate aerobic لازم تكون
+
+175
+00:13:21,960 --> 00:13:28,080
+هوائية إجبارية Oxidative process بتستخدم الأكسجين
+
+176
+00:13:28,080 --> 00:13:31,800
+ك final electron acceptor المستقبل النهائي
+
+177
+00:13:31,800 --> 00:13:35,340
+للإلكترونات بيكون الأكسجين الأكسجين يعتبر
+
+178
+00:13:35,340 --> 00:13:40,240
+inorganic molecule يبقىفي الـ Aerobic Respiration
+
+179
+00:13:40,240 --> 00:13:43,740
+بأستخدم الأكسجين in organic molecule للأكسجين
+
+180
+00:13:43,740 --> 00:13:47,680
+كمستقبل نهائي للإلكترونات خلال الـ Electron
+
+181
+00:13:47,680 --> 00:13:53,640
+Transport Chain قلنا بيصير اللي هو تفاعل أكسدة
+
+182
+00:13:53,640 --> 00:13:57,720
+واختزال المستقبل النهائي للإلكترونات الأكسجين
+
+183
+00:13:57,720 --> 00:14:01,080
+بيصير في عندي بيكون في عندي أنزيم الـ Oxidase الـ
+
+184
+00:14:01,080 --> 00:14:05,540
+Cytochrome C Oxidase بيعمل أكسدة للـ Cytochrome C
+
+185
+00:14:05,540 --> 00:14:09,950
+Reducedأكسى دا يعني فقد الإلكترونات هياخدوا
+
+186
+00:14:09,950 --> 00:14:13,510
+الإلكترون الـ Oxygen كمستقبل نهائي للإلكترونات
+
+187
+00:14:13,510 --> 00:14:18,970
+ويتكون الـH2O بالنهاية أنا بحصل من هذه العملية على
+
+188
+00:14:18,970 --> 00:14:26,370
+36 لـ 38 ATP pair جليكوز molecule كل جزء جليكوز
+
+189
+00:14:26,370 --> 00:14:34,710
+بينتج 36 ل 38 ATP ليش قالي 36 ل 38؟ 36 في حال كان
+
+190
+00:14:34,710 --> 00:14:40,930
+Eukaryoticالـ 38 في حال كان بره كديوتيك يبقى عملية
+
+191
+00:14:40,930 --> 00:14:45,310
+الـ Aerobic Respiration عملية بتتعامل مع ان الـ
+
+192
+00:14:45,310 --> 00:14:49,170
+Oxygen هو المستقبل النهائي للإلكترونات كمية الطاقة
+
+193
+00:14:49,170 --> 00:14:53,810
+اللي بتنتج من 36 لـ 38 بقوللي هي ال most efficient
+
+194
+00:14:53,810 --> 00:14:58,730
+form of the energy productionبالنسبة لتانية عملية
+
+195
+00:14:58,730 --> 00:15:02,730
+ال fermentation بستخدم organic molecule كمستقبل
+
+196
+00:15:02,730 --> 00:15:05,510
+نهائي للإلكترونات احنا قلنا في ال aerobic
+
+197
+00:15:05,510 --> 00:15:08,590
+respiration بستخدم inorganic molecule اللي هو
+
+198
+00:15:08,590 --> 00:15:12,310
+الأكسجين لكن في ال fermentation بستخدم organic
+
+199
+00:15:12,310 --> 00:15:16,510
+molecule كمستقبل نهائي للإلكترونات زي مين في عندي
+
+200
+00:15:16,510 --> 00:15:20,490
+organic molecule بقولي بشكل رئيسي الكربوهيدرات على
+
+201
+00:15:20,490 --> 00:15:25,100
+سبيل المثال جليكوز، لاكتاز، مالتاز، ساكرازالطاقة
+
+202
+00:15:25,100 --> 00:15:28,840
+اللي بتنتج خلال عملية ال fermentation بقول هي أقل
+
+203
+00:15:28,840 --> 00:15:33,820
+كفاءة من ال aerobic respiration فقط بيتم إنتاج 2
+
+204
+00:15:33,820 --> 00:15:39,780
+ATB بجليكوز molecule كل جليكوز molecule بينتج منه
+
+205
+00:15:39,780 --> 00:15:41,880
+فقط 2ATB
+
+206
+00:15:46,750 --> 00:15:50,170
+يبقى هذا بالنسبة لـ fermentation بأستخدم organic
+
+207
+00:15:50,170 --> 00:15:55,030
+molecule زي الكربوهيدرات كمية الطاقة التي تنتج to
+
+208
+00:15:55,030 --> 00:16:00,510
+ATP ال fermentation بتصير فقط في البكتيريا احنا
+
+209
+00:16:00,510 --> 00:16:03,750
+قولنا ال aerobic respiration فقط لازم تكون
+
+210
+00:16:03,750 --> 00:16:07,990
+البكتيريا هوائية إجبارية في ال fermentation بتكون
+
+211
+00:16:07,990 --> 00:16:12,170
+anaerobic بكتيريا بكتيريا لا هوائية وكمان
+
+212
+00:16:12,170 --> 00:16:17,600
+الفكولتات في anaerobicاللي هي الهوائية الاختيارية
+
+213
+00:16:17,600 --> 00:16:22,500
+ممكن انها تعمل fermentation and
+
+214
+00:16:22,500 --> 00:16:25,400
+aerobic respiration is similar to aerobic
+
+215
+00:16:25,400 --> 00:16:28,580
+respiration بقولي بتشبه ال aerobic respiration
+
+216
+00:16:28,580 --> 00:16:32,760
+لأنها بتستخدم inorganic molecule لكن في ال aerobic
+
+217
+00:16:32,760 --> 00:16:37,040
+respiration احنا قولنا بنستخدم inorganic molecule
+
+218
+00:16:37,040 --> 00:16:40,620
+اللي هو ال A2 لكن هنا انا بستخدم inorganic
+
+219
+00:16:40,620 --> 00:16:47,250
+molecule such as iron منجانيزقابلت نيترات صلفة
+
+220
+00:16:47,250 --> 00:16:51,430
+يبقى هاي اللي بستخدمها في الان أيوروبيك ريسبيراشن
+
+221
+00:16:51,430 --> 00:16:55,570
+ال inorganic molecule لكن في ال أيوروبيك بستخدم
+
+222
+00:16:55,570 --> 00:16:59,870
+الأكسجين كمستقبل نهائي للإلكترونات كمية الطاقة
+
+223
+00:16:59,870 --> 00:17:04,630
+اللي يتم إنتاجها لكل جزاء جيليوكوز بقول بتختلف حسب
+
+224
+00:17:04,630 --> 00:17:08,430
+نوع المستقبل النهائي للإلكترونات لو أنا استخدمت
+
+225
+00:17:08,430 --> 00:17:11,810
+ايرون كمية الطاقة بتختلف عن لو أنا استخدمت قابل
+
+226
+00:17:11,810 --> 00:17:18,080
+هذا بالنسبة لالان aerobic respiration كمية الطاقة
+
+227
+00:17:18,080 --> 00:17:22,580
+التي تنتج حسب المستقبل النهائي للإلكترونات يبقى ال
+
+228
+00:17:22,580 --> 00:17:25,280
+aerobic respiration بستخدم inorganic molecule
+
+229
+00:17:25,280 --> 00:17:30,440
+الأكسجين كمية الطاقة من 36 ل 38 ATP الآن aerobic
+
+230
+00:17:30,440 --> 00:17:34,660
+respiration بستخدم inorganic molecule زي ال iron
+
+231
+00:17:34,660 --> 00:17:38,360
+المنجنيز الكوبالت النيترات السلفات كمية الطاقة حسب
+
+232
+00:17:38,360 --> 00:17:42,350
+المستقبل النهائي للإلكتروناتالـ fermentation
+
+233
+00:17:42,350 --> 00:17:46,410
+باستخدم organic molecules زي الكربوهايدرات جمية
+
+234
+00:17:46,410 --> 00:17:51,810
+الطاقة فقط 2 ATP لكل جزاء جليكوز أخر جملة موجودة
+
+235
+00:17:51,810 --> 00:17:55,330
+في هاي ال slide بقول All fermentative bacteria can
+
+236
+00:17:55,330 --> 00:17:58,530
+ferment the simplest sugar اللي هو الديكستروز أو
+
+237
+00:17:58,530 --> 00:18:02,170
+الديجليكوز كل البكتيريا اللي انتها القدرة انها
+
+238
+00:18:02,170 --> 00:18:06,250
+تعمل ال fermentation بقول بتقدر تعمل fermentation
+
+239
+00:18:06,250 --> 00:18:10,740
+لأبسط سكر اللي هو الجليكوزالجليكوز قلنا
+
+240
+00:18:10,740 --> 00:18:15,580
+monosaccharide يبقى هو simple sugar بسيط فأي
+
+241
+00:18:15,580 --> 00:18:19,280
+بكتيريا بتقدر انها تعمل fermentation هتكون بتعمل
+
+242
+00:18:19,280 --> 00:18:23,180
+fermentation لأبسط ال sugar اللي هو الجليكوز
+
+243
+00:18:23,180 --> 00:18:27,800
+اللكتوز قلنا disaccharide اللي هو بتكون من two
+
+244
+00:18:27,800 --> 00:18:33,800
+sugar البكتيريا لو عملت تخمير للكتوز بتكون بالاصل
+
+245
+00:18:33,800 --> 00:18:38,900
+هي عاملة للجليكوز لأن اللكتوز يعتبر disaccharide
+
+246
+00:18:39,420 --> 00:18:43,100
+سكر معقد فأكيد بما أنها قدرت تعمل fermentation
+
+247
+00:18:43,100 --> 00:18:48,000
+للسكر المعقد هتكون عملت fermentation لل sugar
+
+248
+00:18:48,000 --> 00:18:52,640
+الأبسط منه اللي هو الجليكوز المونوسكري لكن العكس
+
+249
+00:18:52,640 --> 00:18:58,280
+مش صحيح يعني البكتيريا لو ماقدرت تعمل تخميد للكتاز
+
+250
+00:18:58,280 --> 00:19:01,700
+ممكن بالأصل تكون هي gelicose fermenter
+
+251
+00:19:06,660 --> 00:19:11,660
+النون لكتاز فيرمنتر هي مابتقدر تعمل تخميد للكتاز
+
+252
+00:19:11,660 --> 00:19:17,200
+لكن قدرت تعمل تخميد للجليكوز للسكر البسيطممكن انها
+
+253
+00:19:17,200 --> 00:19:20,660
+تكون في الاصل هي non-lactose fermenter وكمان non
+
+254
+00:19:20,660 --> 00:19:24,500
+-gelicose fermenter زي الصدومونس الصدومونس هي non
+
+255
+00:19:24,500 --> 00:19:28,080
+-gelicose fermenter فأكيد مش هتقدر تعمل تخمير
+
+256
+00:19:28,080 --> 00:19:32,320
+للسكر المعقد ماقدرت تعمل تخمير للسكر البسيط فاشي
+
+257
+00:19:32,320 --> 00:19:36,280
+طبيعي مش هتقدر تعمل تخمير للسكر المعقد اللاكتوز
+
+258
+00:19:36,280 --> 00:19:40,520
+يبقى بقول نهان انه اي بكتيريا بتعمل fermentation
+
+259
+00:19:40,520 --> 00:19:44,940
+هتكون بتقدر تعمل fermentation ل ال simplest sugar
+
+260
+00:19:44,940 --> 00:19:50,480
+اللي هو الجليكوزلو كانت عاملة تخمير لسكر معقد بدي
+
+261
+00:19:50,480 --> 00:19:56,680
+أفهم بشكل بديهي أنه هي قدرت تعمل تخمير للسكر
+
+262
+00:19:56,680 --> 00:20:02,320
+البسيط اللي هو الجليكوز لكن العكس مش صحيح ممكن
+
+263
+00:20:02,320 --> 00:20:07,000
+أنها تكون تقدر تعمل وممكن ما تكون تقدر تعمل
+
+264
+00:20:07,000 --> 00:20:12,320
+fermentation للجليكوز زي اللكتوزقلنا في الانتيرو
+
+265
+00:20:12,320 --> 00:20:16,100
+بكتيريا C في عندي نون لكتوز فرمنتر لكن هي في الأصل
+
+266
+00:20:16,100 --> 00:20:20,260
+قدرت تعمل للجليكوز في عندي نون لكتوز فرمنتر زي
+
+267
+00:20:20,260 --> 00:20:28,340
+السودومونس لكن هي بالأصل مابتقدر تعمل للجليكوز طيب
+
+268
+00:20:28,340 --> 00:20:31,220
+كيف بدي أنا أعرف هل البكتيريا عملت fermentation
+
+269
+00:20:31,220 --> 00:20:35,040
+ولا لأ هذا فحص اللي هو البكتيريا ال fermentation
+
+270
+00:20:35,040 --> 00:20:38,830
+كيف أنا بدي أعمل هذا الفحصالبرنسب اللي هذا الفحص
+
+271
+00:20:38,830 --> 00:20:41,690
+قولنا انه البكتيريا اللي بتقدر تعمل fermentation
+
+272
+00:20:41,690 --> 00:20:45,950
+أكيد هي تشتغل على ال substrate اللي هي
+
+273
+00:20:45,950 --> 00:20:50,070
+الكربوهايدرات هنحط طبعا جليكوز اللي هو أبسط sugar
+
+274
+00:20:50,070 --> 00:20:53,910
+في ال media كربوهايدرات نواتج عملية ال
+
+275
+00:20:53,910 --> 00:20:56,390
+fermentation أكيد ال fermentation هتشتغل على
+
+276
+00:20:56,390 --> 00:21:00,230
+الكربوهايدرات نواتج العملية acidic or alcohol
+
+277
+00:21:00,230 --> 00:21:04,370
+alcohol or gases and product نواتج العملية acid or
+
+278
+00:21:04,370 --> 00:21:07,980
+alcohol or gasكيف طريقة الشغل بدي احضر
+
+279
+00:21:07,980 --> 00:21:12,680
+fermentation tube بتكون هي ال broth مكونات هذه ال
+
+280
+00:21:12,680 --> 00:21:18,060
+tube ال nutrient ال broth ال nutrient ال broth اي
+
+281
+00:21:18,060 --> 00:21:21,100
+media سواء ال nutrient اجارة او ال nutrient ال
+
+282
+00:21:21,100 --> 00:21:25,200
+broth بتحتوي على مصادر للتغذية عشان تقدر تنمو
+
+283
+00:21:25,200 --> 00:21:28,960
+البكتيريا من مصادر التغذية هي في عندي بيبتون كمصدر
+
+284
+00:21:28,960 --> 00:21:33,770
+لل نيتروجين قلنا في عندي ممكن كمانBeef Extract
+
+285
+00:21:33,770 --> 00:21:40,290
+كمصادر لـ Carbon فين دي Phenol Red Phenol Red BH
+
+286
+00:21:40,290 --> 00:21:48,530
+Indicator بتركيز 0.00189% BH Indicator لأنه احنا
+
+287
+00:21:48,530 --> 00:21:52,450
+قلنا نواتج العملية acidic or alcohol فعشان اكشف عن
+
+288
+00:21:52,450 --> 00:21:57,060
+نواتج هايأو نواتج الدفاع اللى بستخدم BH Indicator
+
+289
+00:21:57,060 --> 00:22:00,420
+مين الباقي BH Indicator اللى بستخدمه اللى هو ال
+
+290
+00:22:00,420 --> 00:22:06,220
+Phenol Red بتركيز 0.00189
+
+291
+00:22:06,220 --> 00:22:10,440
+ال substrate اللى هيشتغل عليها الانزيم لازم تكون
+
+292
+00:22:10,440 --> 00:22:17,040
+في ال media اللى هى الجليكوز بنسبة 1.2% يبقى فيه
+
+293
+00:22:17,040 --> 00:22:20,720
+اندي أكيد هيكون جليكوز طيب احنا قلنا بدنا نكشف على
+
+294
+00:22:20,720 --> 00:22:25,510
+ال acid or الكحولمن خلال الفينار Red طب كيف بدي
+
+295
+00:22:25,510 --> 00:22:29,030
+أكشف على الجاز بقول في عندي درهام تيوب هي اللي
+
+296
+00:22:29,030 --> 00:22:32,750
+هتكشف ليه على ال gas product بتكون موجودة في
+
+297
+00:22:32,750 --> 00:22:38,890
+التيوب بتكون
+
+298
+00:22:38,890 --> 00:22:42,430
+موجودة بمقلوبة في التيوب بنحطها هنا
+
+299
+00:22:50,370 --> 00:22:55,450
+هذه الجرهام تيوب بحطها بشكل مقلوب هتقدر تكشفلي على
+
+300
+00:22:55,450 --> 00:23:00,730
+ال gas product طبعا انا بحطها قبل ما ادخلها اوتو
+
+301
+00:23:00,730 --> 00:23:05,970
+كلف لانه اذا كان موجود في عندي air bubbles في هاي
+
+302
+00:23:05,970 --> 00:23:09,270
+ال tube مقبل ال incubation مقبل ان انا ازرع ال
+
+303
+00:23:09,270 --> 00:23:13,130
+اوتو كلف هيتخلص من هاي ال air bubble عشان مايصير
+
+304
+00:23:13,130 --> 00:23:17,650
+في عندي اي false result لانهكيف النتيجة بتكون
+
+305
+00:23:17,650 --> 00:23:21,010
+بيكون عندي إذا كانت هي قدرت تنتج غاز بيكون في عندي
+
+306
+00:23:21,010 --> 00:23:25,450
+air bubbles في الـ tube عشان أمنع الـ false
+
+307
+00:23:25,450 --> 00:23:28,630
+positive result لازم أني أنا أدخلها الـ autoclave
+
+308
+00:23:28,630 --> 00:23:31,270
+بعدين أني أنا أزرع عشان إذا كان في عندي أي air
+
+309
+00:23:31,270 --> 00:23:36,090
+bubbles موجود ما قبل الinculation ال autoclave أنا
+
+310
+00:23:36,090 --> 00:23:40,690
+أتخلص منه من خلال الـ autoclave هي يبقى مكونات ال
+
+311
+00:23:40,690 --> 00:23:47,020
+media اللي فيه نوردشو الألوان تبعته بقول لي في non
+
+312
+00:23:47,020 --> 00:23:50,380
+-red في ال acidic بيكون لونه ياله في ال basic
+
+313
+00:23:50,380 --> 00:23:53,840
+بيكون لونه red في ال neutral بيكون لونه orange إذا
+
+314
+00:23:53,840 --> 00:23:59,740
+كان أقل من 86 بيكون acidic ما بين 86 و 84 بيكون ال
+
+315
+00:23:59,740 --> 00:24:04,600
+neutral إذا كان أعلى من 84 بيكون basic بيكون لونه
+
+316
+00:24:04,600 --> 00:24:09,960
+أحمرهعمل انكيولات لأ البكتيريا اللى انا بدي افحص
+
+317
+00:24:09,960 --> 00:24:15,260
+هل بتقدر تعمل fermentation ولا لأ هايبروث هاخد ال
+
+318
+00:24:15,260 --> 00:24:20,800
+ال ال انيكولام و هعمل mix في هاي ليتيوب هعمل
+
+319
+00:24:20,800 --> 00:24:27,300
+incubation لمدة 24 ساعة على 37 درجة سيليسيا بعد 24
+
+320
+00:24:27,300 --> 00:24:32,200
+ساعة بقولي النتائج بتكون كالتالي اما هتتغير لون
+
+321
+00:24:32,200 --> 00:24:37,110
+ليتيوب لللون ال yellow اش يعنيبقولي هذا معناه ان
+
+322
+00:24:37,110 --> 00:24:41,570
+البكتيريا قدرت تعمل fermentation للجليكوز نواتج
+
+323
+00:24:41,570 --> 00:24:45,730
+عملية ال fermentation أسدك اتكون أسد فبالتالي
+
+324
+00:24:45,730 --> 00:24:49,470
+هيعمل على خفض ال بي اتش وهيتحول اللون للون ييلو
+
+325
+00:24:49,470 --> 00:24:53,090
+ييلو زائد air bubble ال air bubble هتكون في
+
+326
+00:24:53,090 --> 00:24:55,690
+الدرهمت يوب بقولي ان البكتيريا قدرت تعمل
+
+327
+00:24:55,690 --> 00:24:59,330
+fermentation للجليكوز نواتج عملية ال fermentation
+
+328
+00:24:59,330 --> 00:25:04,350
+أسد قللت ال بي اتش اتحول اللون للون أصفروبنفس
+
+329
+00:25:04,350 --> 00:25:07,210
+الوقت كمان كان من نواتج عملية ال fermentation
+
+330
+00:25:07,210 --> 00:25:11,850
+أنتجت gas كيف بدي أكشف عنه من خلال الدرهام tube
+
+331
+00:25:11,850 --> 00:25:15,170
+هشوف air bubbles في الدرهام tube يبقى هاي النتيجة
+
+332
+00:25:15,170 --> 00:25:19,470
+بتكون نواتج التفاعل أو نواتج ال fermentation يكون
+
+333
+00:25:19,470 --> 00:25:25,850
+acid with gas لكن هاي acid without gas Orange زائد
+
+334
+00:25:25,850 --> 00:25:30,030
+air bubblesأورنج هلأ عملت fermentation ولا لأ لأ
+
+335
+00:25:30,030 --> 00:25:33,130
+لأن الأورنج بيكون feeling neutral ماتكون عندي
+
+336
+00:25:33,130 --> 00:25:38,370
+product acidic طيب كيف نتج الغاز عندي و هو من
+
+337
+00:25:38,370 --> 00:25:42,110
+نواتج عملية ال fermentation بيقول لي هان بيكون مش
+
+338
+00:25:42,110 --> 00:25:46,250
+مسكرين الأنبوبة امنيح بيكون الغاز من برة مصر في
+
+339
+00:25:46,250 --> 00:25:50,130
+عندي fermentation لكن ال air bubbles اتكون لنتيجة
+
+340
+00:25:50,130 --> 00:25:56,070
+ان ال tube كانت مش مسكرة امنيح ال red اللون الأحمر
+
+341
+00:25:56,300 --> 00:25:58,920
+أكيد مش هيكون في عندي fermentation لأن اللون
+
+342
+00:25:58,920 --> 00:26:05,560
+الأحمر موجود في ال basic في حال كان في عندي
+
+343
+00:26:05,560 --> 00:26:10,360
+alkaline ما أصبح في عندي fermentation طيب شو سبب
+
+344
+00:26:10,360 --> 00:26:14,560
+اللون الأحمر بقول لي أنه يكون نتيجة ال deamination
+
+345
+00:26:14,560 --> 00:26:18,140
+of بيبتون من مكونات ال media في عندي بيبتون
+
+346
+00:26:18,140 --> 00:26:22,220
+البيبتون هذاالبكتيريا اللى ماقدرت تعمل
+
+347
+00:26:22,220 --> 00:26:25,880
+fermentation للجليكوز هتروح لمصادر تانية هتروح
+
+348
+00:26:25,880 --> 00:26:31,520
+للبيبتون تعمل deamination هتنزع الـ N-H3
+
+349
+00:26:31,520 --> 00:26:36,160
+Deamination ايش معناها انها تنزع الامانيا البيبتون
+
+350
+00:26:36,160 --> 00:26:41,140
+اللى هو ال amino acid ال amino acid احد اللى هو
+
+351
+00:26:41,140 --> 00:26:46,380
+مكوناته او ال structure سبعته بيكون فيه اندى N-H3
+
+352
+00:26:47,020 --> 00:26:50,260
+البكتيريا هتعمل الـ Deamination هتنزع الـ NH3
+
+353
+00:26:50,260 --> 00:26:54,300
+الأمونيا عشان تحصل على نيتروجين سارس تقدر تنمو
+
+354
+00:26:54,300 --> 00:26:58,820
+وتتكثر اتكون الـ NH3 أمونيا الأمونيا الالكالاين
+
+355
+00:26:58,820 --> 00:27:05,080
+هيرفع الـ pH وتتحول لون ال ميديا للون أحمر هيك
+
+356
+00:27:05,080 --> 00:27:08,680
+بنكون خلصنا ال bacterial fermentation قولنا ال
+
+357
+00:27:08,680 --> 00:27:11,480
+bacterial fermentation عشان ال principle انه
+
+358
+00:27:11,480 --> 00:27:15,520
+carbohydrate الشيليوكاز بيكون موجود في الميديالو
+
+359
+00:27:15,520 --> 00:27:18,300
+البكتيريا قدرت تعمل fermentation النواتج هتكون
+
+360
+00:27:18,300 --> 00:27:22,700
+acid or alcohol or gas كيف بدي أكشف عن ال acid؟ في
+
+361
+00:27:22,700 --> 00:27:26,160
+عندي pH indicator اللي هو الفينار Red كيف بدي أكشف
+
+362
+00:27:26,160 --> 00:27:29,640
+على ال gas؟ في عندي درهام تيوب بقدر أكشف على ال
+
+363
+00:27:29,640 --> 00:27:36,020
+gas عرفنا كل launch و تفسير هذه الألوان بالنسبة
+
+364
+00:27:36,020 --> 00:27:39,600
+للـ Significant لهذا الفحص الـ Significant لهذا
+
+365
+00:27:39,600 --> 00:27:43,660
+الفحص اللي هو هيميز بين اللي قلناه ال gelicose
+
+366
+00:27:43,660 --> 00:27:48,090
+fermenterبين positive و negative بين الصدومونس وال
+
+367
+00:27:48,090 --> 00:27:53,910
+انتيرو بكترياسي كمان في اندي في ال انتيرو بكترياسي
+
+368
+00:27:53,910 --> 00:27:56,690
+ممكن انها تميز ال lactose fermenter عن ال non
+
+369
+00:27:56,690 --> 00:27:59,210
+lactose fermenter هذا بالنسبة ل significant
+
+370
+00:28:08,880 --> 00:28:11,960
+Fermentation of Carbohydrate Carbohydrate are
+
+371
+00:28:11,960 --> 00:28:15,680
+complex chemical substrate which serves as energy
+
+372
+00:28:15,680 --> 00:28:19,880
+source قولنا إن الـ Carbohydrate هي معقدة بتكون
+
+373
+00:28:19,880 --> 00:28:24,380
+مصدر للطاقة و كمان لـ Carbon Source لما تتكسر
+
+374
+00:28:24,380 --> 00:28:28,880
+بواسطة البكتيريا and other cells أكيد أنا لما
+
+375
+00:28:28,880 --> 00:28:32,640
+تتكسر بحصل عليه من خلالها علاقة طاقة و بحصل على
+
+376
+00:28:32,640 --> 00:28:35,640
+Carbon Source هذا الـ Carbon Source مهم لنموء
+
+377
+00:28:35,640 --> 00:28:40,530
+الخلية الخلية البكتيريةمكونات الـ Carbohydrate,
+
+378
+00:28:40,630 --> 00:28:46,170
+Carbon, Hydrogen and Oxygen حسب هذه العلاقة ممكن
+
+379
+00:28:46,170 --> 00:28:49,810
+أنه يكون صنفه إلى Sugar or Starch الـ Sugar اللي
+
+380
+00:28:49,810 --> 00:28:52,970
+هي الـ Monodiosaccharide، الـ Starch قولنا الـ
+
+381
+00:28:52,970 --> 00:28:54,210
+Polysaccharide
+
+382
+00:28:56,080 --> 00:29:00,240
+facultative anaerobic and anaerobic bacteria are
+
+383
+00:29:00,240 --> 00:29:02,540
+capable of fermentation قولت أنا عملية ال
+
+384
+00:29:02,540 --> 00:29:05,560
+fermentation بتقدر اللي هي ال facultative
+
+385
+00:29:05,560 --> 00:29:09,620
+anaerobic and anaerobic bacteria قولنا ال
+
+386
+00:29:09,620 --> 00:29:13,420
+anaerobic respiration فقط ال obligate anaerobic هي
+
+387
+00:29:13,420 --> 00:29:17,060
+اللي بتقدر تعمل ال anaerobic respiration لكن ال
+
+388
+00:29:17,060 --> 00:29:20,780
+fermentation ال anaerobic bacteria و ال
+
+389
+00:29:20,780 --> 00:29:23,560
+facultative anaerobic bacteria بتقدر تعمل
+
+390
+00:29:23,560 --> 00:29:28,050
+fermentationالفكولتات الـ anaerobic هي الهوائية
+
+391
+00:29:28,050 --> 00:29:32,390
+الإختيارية لأن الـ fermentation يقولنا بيحصل في
+
+392
+00:29:32,390 --> 00:29:35,870
+ظروف لا هوائية عشان هيك أشي طبيعي الـ anaerobic
+
+393
+00:29:35,870 --> 00:29:38,830
+بكتيريا تقدر تعمل الـ fermentation الفكولتات الـ
+
+394
+00:29:38,830 --> 00:29:43,090
+anaerobic في حال ما عندي أكسجين أكيد بتستطيع العيش
+
+395
+00:29:43,090 --> 00:29:48,290
+بدون أكسجين فبالتالي بتقدر تعمل fermentationلكن
+
+396
+00:29:48,290 --> 00:29:52,310
+الـ Obligate Aerobic Bacteria ما بتقدر تعمل
+
+397
+00:29:52,310 --> 00:29:56,030
+fermentation ل الجليكوز ده هي الصدومونس الصدومونس
+
+398
+00:29:56,030 --> 00:30:00,490
+تعتبر Obligate Aerobic تمام قلنا هي Non-Gelicose
+
+399
+00:30:00,490 --> 00:30:04,370
+Fermenter شو تعريف عملية ال fermentation هاني معرف
+
+400
+00:30:04,370 --> 00:30:09,530
+عملية ال fermentation Un-Aerobic Process هي عملية
+
+401
+00:30:09,530 --> 00:30:12,190
+لا هوائية عشان هي فقط اللي بتقدر تعمل ال
+
+402
+00:30:12,190 --> 00:30:15,210
+fermentation ال Un-Aerobic Bacteria والفكولتات في
+
+403
+00:30:15,210 --> 00:30:18,710
+Un-Aerobicما بتقدر تعمل الـ obligate aerobic زي
+
+404
+00:30:18,710 --> 00:30:22,690
+الـ Sodomonas during which carbohydrates are
+
+405
+00:30:22,690 --> 00:30:26,690
+broken down for energy production بيقوللي بيتم
+
+406
+00:30:26,690 --> 00:30:36,390
+تكسير ال carbohydrates عشان أحصل على الطاقة a
+
+407
+00:30:36,390 --> 00:30:40,370
+wide variety of carbohydrates may be fermented by
+
+408
+00:30:40,370 --> 00:30:44,240
+various bacteria in order to obtain energyبقولي
+
+409
+00:30:44,240 --> 00:30:50,440
+كتير من ال carbohydrate بيتم تخميرها بواسطة أنواع
+
+410
+00:30:50,440 --> 00:30:54,800
+مختلفة من البكتيريا عشان أحصل على الطاقة and the
+
+411
+00:30:54,800 --> 00:30:57,240
+types of carbohydrate بقولي الأنواع ال
+
+412
+00:30:57,240 --> 00:31:00,420
+carbohydrate اللي بيتم تخميرها by specific
+
+413
+00:31:00,420 --> 00:31:04,320
+organism can service as a diagnostic tool for the
+
+414
+00:31:04,320 --> 00:31:07,840
+identification of that organism قولناحسب
+
+415
+00:31:07,840 --> 00:31:11,040
+الكربوهايدرات اللى بيتم تخميرها وحسب الـ end
+
+416
+00:31:11,040 --> 00:31:14,160
+product أنا هدولا للاتنين بقدر استخدمهم ك
+
+417
+00:31:14,160 --> 00:31:18,400
+diagnostic tool عشان أقدر أعرف البكتيريا ممكن ال
+
+418
+00:31:18,400 --> 00:31:21,920
+ال interior bacteriasi صنفناها لـ lactose
+
+419
+00:31:21,920 --> 00:31:25,000
+fermenter و non lactose fermenter من خلال هذا
+
+420
+00:31:25,000 --> 00:31:28,800
+الفحص بقدر نقسم ال interior bacteriasi لمجموعات
+
+421
+00:31:28,800 --> 00:31:33,190
+وهذا هيساعدني في ال identificationكمان نواتش عملية
+
+422
+00:31:33,190 --> 00:31:37,310
+ال fermentation قلنا ممكن يكون acid or acid with
+
+423
+00:31:37,310 --> 00:31:40,110
+gas فبالتالي برضه هذا هيساعدني في ال
+
+424
+00:31:40,110 --> 00:31:46,530
+identification للorganism نواتش عملية ال
+
+425
+00:31:46,530 --> 00:31:49,710
+fermentation ممكن يكون acid and product وممكن يكون
+
+426
+00:31:49,710 --> 00:31:54,860
+acid with gas and productعشان اكشف على الـ acid
+
+427
+00:31:54,860 --> 00:31:58,540
+production بقول من خلال الـ pH Indicator اللي هو
+
+428
+00:31:58,540 --> 00:32:02,580
+الفينول Red نحفظ حدود الفينول Red في ال acidic
+
+429
+00:32:02,580 --> 00:32:06,040
+بيكون لونه Yellow في ال basic بيكون لونه Red
+
+430
+00:32:06,040 --> 00:32:10,060
+بيتحول إذا كان في عندي acidic product بتتحول لون
+
+431
+00:32:10,060 --> 00:32:15,360
+ال media للون Yellow إذا كان ال ال pH أقل من 6 و 8
+
+432
+00:32:15,360 --> 00:32:19,820
+عشان أكشف على الجاز بحط درهم إتيوب هاي الدرهم
+
+433
+00:32:19,820 --> 00:32:23,400
+إتيوب في عندي اللي هي إتيوب مقلوبةإذا شوفت في عندي
+
+434
+00:32:23,400 --> 00:32:27,600
+air bubbles يبقى هان في عندي gas كان من نواتج
+
+435
+00:32:27,600 --> 00:32:31,800
+عملية ال fermentation بكتيريا produce acidic
+
+436
+00:32:31,800 --> 00:32:34,620
+product when they ferment certain carbohydrate
+
+437
+00:32:34,620 --> 00:32:39,720
+بقوللي أن البكتيريا بتنتج acid product لما اتخمر
+
+438
+00:32:39,720 --> 00:32:42,920
+أنواع معينة من ال carbohydrate ال carbohydrate
+
+439
+00:32:42,920 --> 00:32:46,740
+utilization test فحص ال carbohydrate utilization
+
+440
+00:32:46,740 --> 00:32:49,500
+اللي أنا شرحته قبل شوية
+
+441
+00:32:57,570 --> 00:33:01,090
+مصمم من خلال اني انا اكشف على التغير في ال بي اتش
+
+442
+00:33:01,090 --> 00:33:05,350
+اذا صار في عندي fermentation ولا لأ التغير في ال
+
+443
+00:33:05,350 --> 00:33:09,790
+بي اتش لانه ال fermentation نواتج ال fermentation
+
+444
+00:33:09,790 --> 00:33:14,370
+acid فبالتالي اذا تغير ال بي اتش يبقى صار في عندي
+
+445
+00:33:14,370 --> 00:33:18,880
+fermentationacid lowers the pH of the media بقوللي
+
+446
+00:33:18,880 --> 00:33:22,160
+ان ال acid بيعمل لخفض ال pH في ال media فبالتالي
+
+447
+00:33:22,160 --> 00:33:26,720
+بيخلي ال pH indicator يتحول لللون ال yellow لو
+
+448
+00:33:26,720 --> 00:33:30,280
+البكتيريا ماقدرت اتخمر ال carbohydrate بقوللي ان
+
+449
+00:33:30,280 --> 00:33:34,300
+ال media هيظل لونها أحمر او هتعمل deamination ل
+
+450
+00:33:34,300 --> 00:33:39,020
+الببطان هتنزع ال ammonia ال NH3 ال NH3 alkaline
+
+451
+00:33:39,020 --> 00:33:43,920
+فبالتالي هيتحول لون ال media لللون الأحمر if gas
+
+452
+00:33:43,920 --> 00:33:48,700
+is produced as byby product of fermentation then
+
+453
+00:33:48,700 --> 00:33:52,040
+the Durham tube will have a bubble in it بقولي لو
+
+454
+00:33:52,040 --> 00:33:56,860
+اتكون gas من ال fermentation انا بقدر اكشف عليه من
+
+455
+00:33:56,860 --> 00:34:03,000
+خلال اني انا بشوف air bubbles في ال Durham tube ال
+
+456
+00:34:03,000 --> 00:34:05,800
+carbohydrate اللي ممكن اني انا اكشف عنها جليكوز
+
+457
+00:34:05,800 --> 00:34:09,260
+لاكتوز ساكروس بقولي all carbohydrate test media
+
+458
+00:34:09,260 --> 00:34:13,160
+should be inoculated with a transfer loop عشان
+
+459
+00:34:13,160 --> 00:34:16,700
+اعمل الفحص بستخدم اللي هو ال transfer tubeهذا
+
+460
+00:34:16,700 --> 00:34:21,700
+بالنسبة للـ result لـ fermentation ملاحظ في tube 1
+
+461
+00:34:21,700 --> 00:34:26,660
+و tube 3 تكون اللون ال yellow يبقى فى صار فى عندي
+
+462
+00:34:26,660 --> 00:34:32,080
+fermentation لجليكوز اتكون أسد اتحول اللون للون
+
+463
+00:34:32,080 --> 00:34:37,040
+أصفر طبعا نتيجة اللى هو ال decrease ل ال pH ملاحظ
+
+464
+00:34:37,040 --> 00:34:42,380
+انه high لونها أخف من high هنتعرف كمان شوية ان في
+
+465
+00:34:42,380 --> 00:34:47,110
+بعض أنواع البكتيريا بتعمل fermentation لالجليكوز
+
+466
+00:34:47,110 --> 00:34:51,770
+وبتنتج كمية كبيرة من ال acid أو بتنتج أحماض بتعمل
+
+467
+00:34:51,770 --> 00:34:57,810
+على خفض البيئة مش بصورة كبيرة فبالتالي بيكون اللون
+
+468
+00:34:57,810 --> 00:35:02,170
+غامق كتير لكن ها نتكون acid لكن مش بصورة كبيرة في
+
+469
+00:35:02,170 --> 00:35:06,790
+عندي air bubbles هاي ال tube اللي بالنص تكون اللون
+
+470
+00:35:06,790 --> 00:35:09,570
+ال red يبقى ما صار في عندي fermentation صار في
+
+471
+00:35:09,570 --> 00:35:15,620
+عندي deamination لأ النجاتيف لفرمنتشن تيوب وان و
+
+472
+00:35:15,620 --> 00:35:24,040
+ثري بوزيتيف لفرمنتشن طيب
+
+473
+00:35:24,040 --> 00:35:30,020
+هلأ لو عملت إجرام استان و طلعت إجرام نيجاتيف بصلة
+
+474
+00:35:30,020 --> 00:35:32,920
+ماهو الخطوة التانية الخطوة التانية أني أنا بدي
+
+475
+00:35:32,920 --> 00:35:36,400
+أعمل فحص ال Oxidase قلنا في المعمل السابق عن هذا
+
+476
+00:35:36,400 --> 00:35:39,490
+الموضوعإذا طلعت positive يبقى فيها عندى احتمال
+
+477
+00:35:39,490 --> 00:35:42,870
+Sodamonase, Vibrio, Campylobacter, Helicobacter,
+
+478
+00:35:43,310 --> 00:35:46,450
+Irimonase قولنا هي Oxidase positive لو كانت
+
+479
+00:35:46,450 --> 00:35:49,370
+negative فيها عندى احتمال Enterobacteraceae و
+
+480
+00:35:49,370 --> 00:35:56,310
+Acinetobacter طيب عشان أفرق بين هدولة ال positive,
+
+481
+00:35:56,550 --> 00:35:58,930
+oxidase positive, oxidase negative إذا طلعت
+
+482
+00:35:58,930 --> 00:36:00,630
+Oxidase positive
+
+483
+00:36:03,420 --> 00:36:06,240
+يبقى عندي احتمال قولنا الـ Vibrio, Campylobacter,
+
+484
+00:36:06,460 --> 00:36:09,260
+Helicobacter, Aeromonas و Sodomonas لو طلعت أكسداز
+
+485
+00:36:09,260 --> 00:36:13,380
+negative انتيروبكترياسي أسينيتوباكتر عشان أفرق ال
+
+486
+00:36:13,380 --> 00:36:16,960
+positive بقولي بتعملي فحص الجليكوز fermentation لو
+
+487
+00:36:16,960 --> 00:36:20,200
+طلعت negative قولنا Sodomonas obligate aerobic
+
+488
+00:36:20,200 --> 00:36:25,840
+فبالتالي أكيد هتكون non-gelicose fermenter لو طلعت
+
+489
+00:36:25,840 --> 00:36:29,040
+positive يبقى في عندي أربع احتمالات Vibrio,
+
+490
+00:36:29,160 --> 00:36:33,060
+Campylobacter, Helicobacter, Aeromonasبالنسبة لأ
+
+491
+00:36:33,060 --> 00:36:36,160
+الأكسداز negative عشان أفرق الـ Enterobacteriase و
+
+492
+00:36:36,160 --> 00:36:38,660
+الـ Acinetobacter برضه بعمل فحص الجليكوز
+
+493
+00:36:38,660 --> 00:36:41,320
+fermentation لو طلعت positive يبقى هي
+
+494
+00:36:41,320 --> 00:36:43,740
+Enterobacteriase الـ Enterobacteriase تعتبر
+
+495
+00:36:43,740 --> 00:36:47,600
+facultative and aerobic كلنا facultative and
+
+496
+00:36:47,600 --> 00:36:51,060
+aerobic بكتيريا بتقدر تعمل fermentation لو طلعت
+
+497
+00:36:51,060 --> 00:36:55,160
+negative يبقى هي Acinetobacterبرضه ال انتيرو
+
+498
+00:36:55,160 --> 00:36:58,460
+بكترياسي قلنا بنصنفها لمجموعتان اللي هي lactose
+
+499
+00:36:58,460 --> 00:37:01,780
+fermenter و non lactose fermenter ممكن ان انا اعمل
+
+500
+00:37:01,780 --> 00:37:06,020
+هذا الفحص عشان اقدر ان انا اسم ال انتيرو بكترياسي
+
+501
+00:37:06,020 --> 00:37:10,420
+لمجموعات وهذا هيساعدني في التعريف كيف فحص ال
+
+502
+00:37:10,420 --> 00:37:14,680
+lactose fermenter هنتعرف عليه في ال differential
+
+503
+00:37:14,680 --> 00:37:19,140
+media من خلال المقنكي المقنكي هي تعتبر
+
+504
+00:37:19,140 --> 00:37:22,440
+differential media هتقدر تتميز اللي lactose و non
+
+505
+00:37:22,440 --> 00:37:26,870
+lactose fermenterيبقى هي حسب النوع الـ
+
+506
+00:37:26,870 --> 00:37:30,010
+carbohydrate أنا الـ type of carbohydrate اقدرت ان
+
+507
+00:37:30,010 --> 00:37:34,970
+انا اعمل identification للبكتيريا هيك بنكون خلصنا
+
+508
+00:37:34,970 --> 00:37:38,770
+اول فحص اللي هو ال bacterial fermentation هلأ
+
+509
+00:37:38,770 --> 00:37:42,810
+هنتعرف على فحص ال methyl red vucus بسكوار وهو
+
+510
+00:37:42,810 --> 00:37:44,850
+بيتعلق برضه في ال fermentation
+
diff --git a/PL9fwy3NUQKwZWgAb_V1ATlRRsH-6i3cPC/zA7UNireE5w.srt b/PL9fwy3NUQKwZWgAb_V1ATlRRsH-6i3cPC/zA7UNireE5w.srt
new file mode 100644
index 0000000000000000000000000000000000000000..f6436a2337772c3ecfcb0f5f2aaea7e974d32744
--- /dev/null
+++ b/PL9fwy3NUQKwZWgAb_V1ATlRRsH-6i3cPC/zA7UNireE5w.srt
@@ -0,0 +1,1172 @@
+
+1
+00:00:00,520 --> 00:00:04,220
+السلام عليكم ورحمة الله وبركاته يعطيكم العافية
+
+2
+00:00:04,220 --> 00:00:08,160
+جميعًا نستكمل معكم محاضرات مساق  Microbiology
+
+3
+00:00:08,160 --> 00:00:12,060
+العملي. في المحاضرة السابقة، تعرّفنا على الستان
+
+4
+00:00:12,060 --> 00:00:15,700
+وقسمنا الصبغات حسب الـ function إلى: simple stain،
+
+5
+00:00:15,700 --> 00:00:18,840
+differential stain، و special stain. في هذا اللقاء
+
+6
+00:00:18,840 --> 00:00:21,720
+سنتناول آخر نوع من أنواع الصبغات، وهو الـ
+
+7
+00:00:21,720 --> 00:00:26,010
+special stain. الـ special stains قلنا تستخدم to view
+
+8
+00:00:26,010 --> 00:00:29,770
+specific parts of bacteria. specific part يعني
+
+9
+00:00:29,770 --> 00:00:35,050
+special components. هذه المكونات، عدم وجودها لا يؤثر
+
+10
+00:00:35,050 --> 00:00:39,000
+على الخلية البكتيرية. في المقابل، يوجد عندي essential
+
+11
+00:00:39,000 --> 00:00:44,060
+components. هي مكونات أساسية، ووجودها يؤثر على الخلية
+
+12
+00:00:44,060 --> 00:00:49,480
+البكتيرية. مثل من essential components؟ السيتوبلازم، الجدار
+
+13
+00:00:49,480 --> 00:00:53,780
+الـ cytoplasm، النواة. كلها تعتبر essential
+
+14
+00:00:53,780 --> 00:00:58,460
+components. الخلية البكتيرية لا تستطيع الاستغناء عنها.
+
+15
+00:00:58,460 --> 00:01:03,600
+لكن بالنسبة للـ special components، من الأمثلة عندي
+
+16
+00:01:03,600 --> 00:01:08,970
+الـ spore، الكبسولة، الأسواط. سنتعرف في هذه المحاضرة
+
+17
+00:01:08,970 --> 00:01:13,090
+على كيفية صباغة الـ special components. عندي الـ
+
+18
+00:01:13,090 --> 00:01:16,470
+endospore stain، الـ capsule stain، و الـ flagella
+
+19
+00:01:16,470 --> 00:01:20,870
+stain. أول special component سنتكلم عنه هو الـ
+
+20
+00:01:20,870 --> 00:01:26,510
+spore. endospore staining، صباغة الـ endospore. the
+
+21
+00:01:26,510 --> 00:01:30,270
+name "endospore" is suggestive of a spore or seed
+
+22
+00:01:30,270 --> 00:01:35,440
+like form. بقول لي أن شكل البوغ يشبه البذور. في جملة
+
+23
+00:01:35,440 --> 00:01:39,640
+أخرى، سمعنا أن الأبواغ في البكتيريا تستخدم
+
+24
+00:01:39,640 --> 00:01:43,800
+للحماية، for protection. لكن في الفطريات تستخدم
+
+25
+00:01:43,800 --> 00:01:50,120
+للتكاثر، كالبذور في النباتات. "endo" means within.
+
+26
+00:01:50,120 --> 00:01:54,560
+يقول لنا: طالما البوغ داخل الخلية، نسميه endo-spore.
+
+27
+00:01:54,560 --> 00:01:59,360
+بعد ذلك، قد تبدأ البكتيريا في التحلل، ويخرج البوغ،
+
+28
+00:01:59,360 --> 00:02:04,460
+ونسميه exo-spore. لكن طالما هو داخل الخلية، نسميه
+
+29
+00:02:04,720 --> 00:02:09,140
+endo-spore. يقول لنا أن البكتيريا، عندما تكون في حالتها
+
+30
+00:02:09,140 --> 00:02:13,740
+الطبيعية، وتكون الظروف مهيأة للتكاثر، نسمي
+
+31
+00:02:13,740 --> 00:02:18,280
+الخلية vegetative cell، خلية نباتية. هي الـ
+
+32
+00:02:18,280 --> 00:02:22,440
+vegetative cell. في حال تغيرت الظروف، وأصبحت غير
+
+33
+00:02:22,440 --> 00:02:28,300
+مناسبة للتكاثر، تقوم البكتيريا بتكوين الأبواغ، الـ
+
+34
+00:02:28,300 --> 00:02:32,460
+spore، كنوع من أنواع الحماية من العوامل الخارجية. في
+
+35
+00:02:32,460 --> 00:02:37,520
+هذه الحالة، عندما تتحول الـ vegetative cell إلى spore، نسمي
+
+36
+00:02:37,520 --> 00:02:42,580
+العملية sporulation. لكن لو تحسنت الظروف، وتم
+
+37
+00:02:42,580 --> 00:02:47,600
+تزويدها بالمغذيات، ستعود الـ spore إلى vegetative cell
+
+38
+00:02:47,600 --> 00:02:52,790
+بعملية، ستعود الـ spore إلى vegetative cell بعملية
+
+39
+00:02:52,790 --> 00:02:57,050
+اسمها germination. إذن، sporulation هي عملية
+
+40
+00:02:57,050 --> 00:03:00,270
+تحويل الـ vegetative cell إلى endospore. لو تحسنت
+
+41
+00:03:00,270 --> 00:03:03,530
+الظروف، ستعود الـ endospore إلى vegetative cell في
+
+42
+00:03:03,530 --> 00:03:09,350
+عملية germination. الـ endospore يعتبر resting
+
+43
+00:03:09,350 --> 00:03:15,600
+structure. يعني resting يعني خامل، يعني non-active. by
+
+44
+00:03:15,600 --> 00:03:19,240
+some bacteria. قال لي some، مش all. لأن قلنا أن
+
+45
+00:03:19,240 --> 00:03:22,800
+البوغ عبارة عن special component. ليست كل أنواع
+
+46
+00:03:22,800 --> 00:03:25,700
+البكتيريا تتكونه.  توجد بكتيريا لديها القدرة على
+
+47
+00:03:25,700 --> 00:03:28,980
+تكوين البوغ، spore-forming bacteria. وفي عندي
+
+48
+00:03:28,980 --> 00:03:33,660
+بكتيريا ليس لديها القدرة على تكوين البوغ، non-
+
+49
+00:03:33,660 --> 00:03:38,940
+spore-forming bacteria. for survival during adverse
+
+50
+00:03:38,940 --> 00:03:43,180
+environmental conditions.  بقول لك: تستخدم للحماية، و
+
+51
+00:03:43,180 --> 00:03:47,920
+للبقاء، survival. وهو البقاء في الظروف البيئية
+
+52
+00:03:47,920 --> 00:03:52,020
+الصعبة. على سبيل المثال، في nutrient limitation، في
+
+53
+00:03:52,020 --> 00:03:57,300
+حال نقص المغذيات، or extreme environment. في حال وجود
+
+54
+00:03:57,300 --> 00:04:02,060
+ظروف بيئية صعبة، مثل الجفاف. the spores are
+
+55
+00:04:02,060 --> 00:04:05,860
+resistant to heat and chemicals.  بقول لي أن الـ spores
+
+56
+00:04:05,860 --> 00:04:10,980
+مقاومة للحرارة وللمواد الكيميائية. لماذا؟ because of
+
+57
+00:04:10,980 --> 00:04:14,260
+a tough outer covering made of the protein keratin.
+
+58
+00:04:14,260 --> 00:04:18,340
+يقول لنا: لأنه محاط ببروتين اسمه الكيراتين. إذن،
+
+59
+00:04:18,340 --> 00:04:22,390
+مصطلح الكيراتين مهم. وجود الكيراتين هو
+
+60
+00:04:22,390 --> 00:04:25,970
+ما يجعل الـ spores مقاومة للحرارة وللمواد
+
+61
+00:04:25,970 --> 00:04:30,090
+الكيميائية. من ضمن المواد الكيميائية، الـ stains،
+
+62
+00:04:30,090 --> 00:04:34,730
+الصبغات. إذن، the keratin also resists staining. إذن،
+
+63
+00:04:34,730 --> 00:04:39,330
+الكيراتين سيكون مقاومًا للـ staining، للصباغة. الـ spore
+
+64
+00:04:39,330 --> 00:04:44,630
+سيكون مقاومًا للصباغة. so extreme measures must be
+
+65
+00:04:44,630 --> 00:04:48,050
+taken to stain a spore.  لذلك، يجب أن نأخذ بعين
+
+66
+00:04:48,050 --> 00:04:54,530
+الاعتبار هذا الأمر، عندما نريد صباغة spore في
+
+67
+00:04:54,530 --> 00:04:59,350
+هذه الشريحة. سنتعرف على خصائص الـ spore، الـ endospore.
+
+68
+00:04:59,350 --> 00:05:03,930
+can remain dormant indefinitely; not reproductive.
+
+69
+00:05:03,930 --> 00:05:09,590
+يقول لي أن الـ endospore يبقى خاملًا مدى الحياة، لكن
+
+70
+00:05:09,590 --> 00:05:13,170
+لو تحسنت الظروف، but germinate quickly when
+
+71
+00:05:13,700 --> 00:05:17,120
+appropriate trigger is applied. لو تحسنت الظروف،
+
+72
+00:05:17,120 --> 00:05:21,220
+قد يحدث الإنبات (germination)، ليتحول الـ spore إلى
+
+73
+00:05:21,220 --> 00:05:26,300
+vegetative cell. لكنه يبقى خاملًا لفترة طويلة جدًا، ولا
+
+74
+00:05:26,300 --> 00:05:31,480
+يتكاثر. non-reproductive. وهو inactive. قلنا أنه
+
+75
+00:05:31,480 --> 00:05:35,740
+خامل، dehydrated، يعني منزوع منه الماء. stable for
+
+76
+00:05:35,740 --> 00:05:40,560
+years. قلنا أنه يبقى مستقرًا لسنوات عديدة وطويلة.
+
+77
+00:05:40,560 --> 00:05:45,280
+في حالة تحسن الظروف، حتى بعد مدة طويلة، لديه
+
+78
+00:05:45,280 --> 00:05:49,260
+القدرة to germinate، ويتحول إلى vegetative cell.
+
+79
+00:05:49,260 --> 00:05:53,700
+endospore differs significantly from the
+
+80
+00:05:53,700 --> 00:05:57,580
+vegetative or normally functioning cell. يقول لي أن
+
+81
+00:05:57,580 --> 00:06:01,100
+الـ endospore يختلف كليًا عن الـ vegetative cell.
+
+82
+00:06:01,100 --> 00:06:06,000
+قلنا: inactive، dormant. لكن الـ vegetative cell
+
+83
+00:06:06,000 --> 00:06:09,840
+reproductive، تكاثرية. the spore is stable for
+
+84
+00:06:09,840 --> 00:06:14,240
+years. لكن الـ vegetative cell ليست stable، تمر بـ
+
+85
+00:06:14,240 --> 00:06:19,640
+death phase، تموت، تمر في طور الموت. bacteria can
+
+86
+00:06:19,640 --> 00:06:24,080
+form endospores in approximately six to eight hours
+
+87
+00:06:24,080 --> 00:06:27,980
+after being exposed to adverse conditions.  يقول لي
+
+88
+00:06:27,980 --> 00:06:31,800
+أن البكتيريا تستطيع تكوين الـ spore، طبعًا في حال أصبحت
+
+89
+00:06:31,800 --> 00:06:37,100
+الظروف غير مناسبة للنمو، تقريبًا بعد 6 إلى 8 ساعات من
+
+90
+00:06:37,100 --> 00:06:41,000
+تعرضها لهذه الظروف السيئة. يعني عملية الـ
+
+91
+00:06:41,000 --> 00:06:45,340
+sporulation، تحويل الـ vegetative cell إلى endospore،
+
+92
+00:06:45,340 --> 00:06:51,440
+تتم بعد 6 إلى 8 ساعات من تعرضها لظروف غير مناسبة. germinate
+
+93
+00:06:51,440 --> 00:06:57,130
+into a vegetative cell within 1.5 hours. لكن يقول لنا: في
+
+94
+00:06:57,130 --> 00:07:02,150
+حال تحسنت الظروف، الـ spore سيتحول إلى vegetative cell
+
+95
+00:07:02,150 --> 00:07:06,270
+خلال ساعة ونصف. إذن، عملية germination تتم بعد
+
+96
+00:07:06,270 --> 00:07:10,390
+ساعة ونصف من تحسن الظروف. لذلك، في الجملة الأولى،
+
+97
+00:07:10,390 --> 00:07:14,850
+قال لي أن germinate quickly. عملية germination
+
+98
+00:07:14,850 --> 00:07:18,410
+تحدث بسرعة، بعد ساعة ونصف من تحسن الظروف. لكن الـ
+
+99
+00:07:18,410 --> 00:07:23,050
+sporulation تحدث بعد 6 إلى 8 ساعات من تعرضها
+
+100
+00:07:23,050 --> 00:07:27,680
+للظروف السيئة. يعني germination أسرع من الـ
+
+101
+00:07:27,680 --> 00:07:31,960
+sporulation. Spores
+
+102
+00:07:31,960 --> 00:07:36,580
+formed by Gram-positive bacteria. وقلنا أن
+
+103
+00:07:36,580 --> 00:07:40,180
+ليست كل البكتيريا تتكون spores. أشهر البكتيريا التي
+
+104
+00:07:40,180 --> 00:07:45,160
+تكون spores هي الباسيلس والكلوستريديوم. الباسيلس و
+
+105
+00:07:45,160 --> 00:07:49,670
+الكلوستريديوم، هذان Gram-positive bacteria. الـ Gram
+
+106
+00:07:49,670 --> 00:07:53,750
+positive bacilli، شكلها عصوي، bacilli، تمام.
+
+107
+00:07:53,750 --> 00:07:56,190
+البكتيريا spore-forming،  يقول لنا:
+
+108
+00:07:56,190 --> 00:07:59,730
+البا سيلوس والكلوستريديوم، هي Gram-positive بكتيريا. هذا
+
+109
+00:07:59,730 --> 00:08:03,090
+لا يعني أن كل Gram-positive هي spore-forming.  في
+
+110
+00:08:03,090 --> 00:08:06,310
+عندي بكتيريا Gram-positive لكن ليست spore-forming.
+
+111
+00:08:06,310 --> 00:08:10,570
+سنحكي عن أشهر البكتيريا spore-forming. سنتكلم
+
+112
+00:08:10,570 --> 00:08:15,440
+عنها بشكل مختصر. أول بكتيريا، الـ Bacillus anthracis،
+
+113
+00:08:15,440 --> 00:08:21,000
+التي تسبب Anthrax، الجمرة الخبيثة. الإصابة بهذه
+
+114
+00:08:21,000 --> 00:08:24,720
+البكتيريا قد تكون عن طريق injection أو الـ
+
+115
+00:08:24,720 --> 00:08:27,780
+inhalation، الاستنشاق. استنشاق الـ Bacillus
+
+116
+00:08:27,780 --> 00:08:32,700
+anthracis spores. كان يتم استخدامها في
+
+117
+00:08:32,700 --> 00:08:36,590
+الحروب البيولوجية. يأخذون ظرفًا ويضعون فيه
+
+118
+00:08:36,590 --> 00:08:39,690
+spores هذه البكتيريا، ويرسلونها إلى
+
+119
+00:08:39,690 --> 00:08:43,950
+الدولة المعادية. عندما يصل الظرف، يفتحونه، ويشمون
+
+120
+00:08:43,950 --> 00:08:49,250
+فيستنشقون، وبالتالي يصابون بالـ anthrax، بالجمرة
+
+121
+00:08:49,250 --> 00:08:53,530
+الخبيثة. بالنسبة للكلوستريديوم، في عندي
+
+122
+00:08:53,530 --> 00:08:57,590
+three species تسبب أمراضًا للإنسان. أولًا،
+
+123
+00:08:57,590 --> 00:09:01,940
+Clostridium botulinum، الذي يسبب الـ
+
+124
+00:09:01,940 --> 00:09:06,220
+botulism، وهو الـ food poisoning. يسبب هذا
+
+125
+00:09:06,220 --> 00:09:09,820
+الشلل.  لو شخص أصيب بـ Clostridium
+
+126
+00:09:09,820 --> 00:09:13,920
+botulinum، يحدث في عينيه شلل ارتخائي. لذلك،
+
+127
+00:09:13,920 --> 00:09:19,340
+يستخدم في عمليات التجميل، الـ Botox. يتم حقنه في
+
+128
+00:09:19,340 --> 00:09:24,230
+منطقة، مثل حول العين، ليحدث ارتخاء في
+
+129
+00:09:24,230 --> 00:09:28,170
+عندي نوع آخر من Clostridium، نوع آخر من
+
+130
+00:09:28,170 --> 00:09:32,090
+Clostridium، Clostridium perfringens، التي تسبب gas
+
+131
+00:09:32,090 --> 00:09:36,750
+gangrene، وهي نقص وصول الـ oxygen للأنسجة.
+
+132
+00:09:36,750 --> 00:09:42,190
+فيصبح لون العضو أسود. Clostridium tetani،
+
+133
+00:09:42,190 --> 00:09:45,890
+التي تسبب tetanus، أو له اسم آخر، fixed
+
+134
+00:09:45,890 --> 00:09:49,950
+smile، أو الكزاز. يكون هناك ضغط على أسنانه، ويحدث لديه
+
+135
+00:09:49,950 --> 00:09:53,630
+شلل انقباضي. إذن، Clostridium tetani يسبب
+
+136
+00:09:53,630 --> 00:09:57,210
+شللًا انقباضيًا. لكن Clostridium botulinum
+
+137
+00:09:57,210 --> 00:10:02,710
+يسبب شللًا ارتخائيًا. هذا بالنسبة لأشهر
+
+138
+00:10:02,710 --> 00:10:06,130
+البكتيريا spore-forming. ستتعرفون أكثر على كل
+
+139
+00:10:06,130 --> 00:10:09,530
+بكتيريا، على pathogenesis، طريقة إحداثها للمرض،
+
+140
+00:10:09,530 --> 00:10:14,170
+لكن المطلوب أن تعرفوا أن هذه هي spore-forming. المطلوب
+
+141
+00:10:14,170 --> 00:10:23,650
+أن نعرف اسم المرض الذي تسببه كل بكتيريا. endospore
+
+142
+00:10:23,650 --> 00:10:27,230
+location. موقع الـ endospore. position of the
+
+143
+00:10:27,230 --> 00:10:31,710
+endospore differs among bacterial species and is
+
+144
+00:10:31,710 --> 00:10:36,590
+useful in identification. يقول لي أن موقع
+
+145
+00:10:36,590 --> 00:10:40,010
+الـ endospore يختلف من نوع لآخر. قد يكون
+
+146
+00:10:40,010 --> 00:10:43,950
+central endospore، يقع في المنتصف. قد يكون sub-
+
+147
+00:10:43,950 --> 00:10:47,450
+terminal، قريب من الطرف. قد يكون terminal
+
+148
+00:10:47,450 --> 00:10:51,670
+endospore. من الأمثلة على الـ central، الباسيلوس
+
+149
+00:10:51,670 --> 00:10:55,090
+cereus، والباسيلوس anthracis. terminal،
+
+150
+00:10:55,090 --> 00:10:58,470
+Clostridium tetani. subterminal، الباسيلوس
+
+151
+00:10:58,470 --> 00:11:01,670
+subtilis. إذن، عندي الباسيلوس cereus،
+
+152
+00:11:01,670 --> 00:11:06,110
+والباسيلوس subtilis، أعتبرهما spore-forming
+
+153
+00:11:06,110 --> 00:11:10,570
+bacteria. قلنا أن Gram stain هي أول
+
+154
+00:11:10,570 --> 00:11:14,790
+خطوة في تعريف البكتيريا. لكن لا أستطيع من خلال
+
+155
+00:11:14,790 --> 00:11:19,410
+Gram stain فقط، أن أعرف البكتيريا. يقول لي من
+
+156
+00:11:19,410 --> 00:11:22,390
+خلال endospore stain، أو endospore location،
+
+157
+00:11:22,390 --> 00:11:26,630
+قد يساعد في التعريف. كيف؟ على سبيل المثال، كأن
+
+158
+00:11:26,630 --> 00:11:31,890
+عندي بكتيريا X، أريد التعرف عليها. أول خطوة لأعمل
+
+159
+00:11:31,890 --> 00:11:36,030
+تعريفًا، أجري Gram stain. أجريت Gram stain،
+
+160
+00:11:36,030 --> 00:11:39,790
+وكانت النتيجة Gram-positive bacilli.  حسنًا، عندي Gram-
+
+161
+00:11:39,790 --> 00:11:42,450
+positive bacilli. يعني مجموعة كبيرة من
+
+162
+00:11:42,450 --> 00:11:45,650
+البكتيريا. أعرف أن بعضها قد يكون spore-
+
+163
+00:11:45,650 --> 00:11:49,580
+forming، وبعضها قد يكون non-spore forming. أذهب
+
+164
+00:11:49,580 --> 00:11:56,020
+وأجري endospore stain. طلعت من endospore stain
+
+165
+00:11:56,020 --> 00:12:00,220
+أن البكتيريا spore-forming. سأستبعد كل البكتيريا
+
+166
+00:12:00,220 --> 00:12:04,680
+non-spore-forming من الـ Gram-positive bacilli. سأذهب
+
+167
+00:12:04,680 --> 00:12:08,540
+وأفكر في spore-forming من الـ Gram-positive bacilli.
+
+168
+00:12:08,540 --> 00:12:11,860
+أعرف من الـ spore-forming Gram-positive
+
+169
+00:12:11,860 --> 00:12:17,160
+bacilli، أنهم الباسيلوس والكلوستريديوم.  لأفرق بين
+
+170
+00:12:17,160 --> 00:12:20,320
+البا سيلوس و Clostridium، في فحص كيميائي آخر، سنتعرف
+
+171
+00:12:20,320 --> 00:12:24,740
+عليه في المحاضرات القادمة. حسنًا، أجريت هذا الفحص،
+
+172
+00:12:24,740 --> 00:12:27,860
+وكانت النتيجة أن هذه البكتيريا هي Bacillus. حسنًا، في
+
+173
+00:12:27,860 --> 00:12:31,700
+عندي أنواع من الباسيلوس. كيف أعرف أي نوع هذه
+
+174
+00:12:31,700 --> 00:12:35,560
+البكتيريا؟ يقول لي من خلال موقع الـ endospore،
+
+175
+00:12:35,560 --> 00:12:39,720
+endospore location. سبق أن قلت أن endospore
+
+176
+00:12:39,720 --> 00:12:43,940
+location هو subterminal.  إذن، البكتيريا هي
+
+177
+00:12:43,940 --> 00:12:48,460
+باسيلوس. إذن، من خلال موقع الـ endospore،
+
+178
+00:12:48,460 --> 00:12:54,720
+ساعدني في تعريف البكتيريا
+
+223
+00:15:56,920 --> 00:16:01,590
+مميزاتها أن هي Lipid soluble وهذا اللي ساعدها في
+
+224
+00:16:01,590 --> 00:16:05,990
+اختراق ال wax lipid في ال mycobacterium لكن في
+
+225
+00:16:05,990 --> 00:16:10,190
+الاسبوري ما عندي wax lipid عندي كيراتين سأستخدم
+
+226
+00:16:10,190 --> 00:16:16,030
+Malachite green الملاكايت
+
+227
+00:16:16,030 --> 00:16:20,730
+جرين هي الـ primary stain الملاكايت جرين نعرف أنه
+
+228
+00:16:20,730 --> 00:16:26,850
+هي basic stain ومعلومة إضافية أنها water soluble
+
+229
+00:16:26,850 --> 00:16:29,790
+يبقى نكتب هنا الـ primary stain
+
+230
+00:16:41,160 --> 00:16:44,480
+الـ Primary Stain الملاكايت Green وهي Water
+
+231
+00:16:44,480 --> 00:16:49,320
+Soluble يبقى عشان نحاول أن نحفظ ونقارن الـ Acid
+
+232
+00:16:49,320 --> 00:16:52,280
+Phosphate Stain استخدمت الـ Primary Stain الكاربو
+
+233
+00:16:52,280 --> 00:16:56,400
+الفوكسيني اللي تعتبر Lipid Soluble عشان الـ wax
+
+234
+00:16:56,400 --> 00:16:59,900
+lipid الموجود في الـ Mycobacterium لكن في الـ Indus
+
+235
+00:16:59,900 --> 00:17:03,360
+Pore استخدمت الـ Primary Stain الملاكايت Green اللي
+
+236
+00:17:03,360 --> 00:17:08,540
+هي Water Soluble ليش استخدمتها؟ نعرف في الـ slides
+
+237
+00:17:08,540 --> 00:17:12,810
+الجاية. أول خطوة في الـ staining طبعا احنا قلنا smear
+
+238
+00:17:12,810 --> 00:17:16,050
+preparation and air drying heat fixation بعدها
+
+239
+00:17:16,050 --> 00:17:19,770
+سأصبغ، سأجيب بيكر، أضع فيه ماء، أخليه يغلي، لما يغلي
+
+240
+00:17:19,770 --> 00:17:23,910
+سأضع الـ slide على طرف البيكر بغمر الـ slide بالـ
+
+241
+00:17:23,910 --> 00:17:29,030
+primary stain الملاكايت الـ green سأنتظر من 3 لـ 5
+
+242
+00:17:29,030 --> 00:17:35,570
+دقائق، وبعد ما تنتهي الخمس دقائق طبعا سأنتظر، سأخليها
+
+243
+00:17:35,570 --> 00:17:39,840
+تبرد. الـ steam سيعمل enhance للملاكايت جرين
+
+244
+00:17:39,840 --> 00:17:44,560
+penetration سيساعد على اختراق الملاكايت جرين الصبغة
+
+245
+00:17:44,560 --> 00:17:50,100
+للكيراتين. سأقول، أنا لو جفت الصبغة نفس
+
+246
+00:17:50,100 --> 00:17:52,920
+في الـ acid phosphate أضيف الصبغة لأنها يتكون
+
+247
+00:17:52,920 --> 00:17:57,740
+كريستال لونه أخضر. بعد ما أخلص الخمس دقائق أخليها
+
+248
+00:17:57,740 --> 00:18:02,260
+تبرد. يبقى الخطوة الثانية cooling سأعمل cooling وهي
+
+249
+00:18:02,260 --> 00:18:06,330
+الخطوة مهمة جدا لأن لو ما خليتها تبرد مدة كافية
+
+250
+00:18:06,330 --> 00:18:11,210
+ستؤثر على النتائج. الـ cooling قلنا act as more
+
+251
+00:18:11,210 --> 00:18:15,050
+than يبقى الـ cooling سيكون act as more than
+
+252
+00:18:24,320 --> 00:18:29,160
+تمام. الـ cooling يعتبر كموردنت لأنه من خلال الـ
+
+253
+00:18:29,160 --> 00:18:32,580
+cooling سيكون كموردنت لأنه من خلال الـ heat أو الـ
+
+254
+00:18:32,580 --> 00:18:36,860
+steam انكسرت الروابط في الكيراتين، قدرت الصبغة
+
+255
+00:18:36,860 --> 00:18:41,080
+الملاكايت الـ green تخترق الـ spore حتى أثبت
+
+256
+00:18:41,080 --> 00:18:44,760
+الملاكايت الـ green في الـ spore أخليها تبرد لأن الـ
+
+257
+00:18:44,760 --> 00:18:48,280
+cooling سيعمل re-naturation للروابط، بالتالي الصبغة
+
+258
+00:18:48,280 --> 00:18:53,280
+ستثبت. بعد الـ cooling gently rinse the slide with
+
+259
+00:18:53,280 --> 00:18:56,760
+water. الخطوة بعد ما نستخدم الـ primary stain
+
+260
+00:18:56,760 --> 00:19:01,480
+mordant بعدين نستخدم الـ decolorizer. الـ decolorizer
+
+261
+00:19:01,480 --> 00:19:05,460
+في الـ endospore stain هو الـ water يبقى الـ
+
+262
+00:19:05,460 --> 00:19:07,220
+decolorizer هو الـ water
+
+263
+00:19:22,030 --> 00:19:26,530
+الـ water سأعتبره كـ decolorizer طب ليش؟ احنا قلنا
+
+264
+00:19:26,530 --> 00:19:31,250
+أن الـ primary stain هي الملاكايت green واتفقنا على
+
+265
+00:19:31,250 --> 00:19:35,710
+معلومة أن الملاكايت green هي water soluble يبقى
+
+266
+00:19:35,710 --> 00:19:39,810
+الماء سيُزيل الملاكايت green سيُزيل الصبغة بدون
+
+267
+00:19:39,810 --> 00:19:44,830
+الحاجة لأي مادة كيميائية. يبقى عرفنا ليش أنا اخترت
+
+268
+00:19:44,830 --> 00:19:48,890
+بالذات الملاكايت green كـ primary stain لأنها water
+
+269
+00:19:48,890 --> 00:19:54,160
+soluble واحنا ما استخدمنا أي مادة كيميائية اعتبرنا
+
+270
+00:19:54,160 --> 00:19:58,440
+الـ water هو الـ decolorizer بعدها سأضيف الـ
+
+271
+00:19:58,440 --> 00:20:04,800
+counterstain الصفرانين لمدة من 1 لـ 2 من 1 لـ 2
+
+272
+00:20:04,800 --> 00:20:09,800
+minute بعدها سأغسل بالـ water سأعمل air drying على الـ
+
+273
+00:20:09,800 --> 00:20:12,920
+oil immersion يبقى الصفرانين هي counterstain
+
+274
+00:20:38,790 --> 00:20:43,270
+يبقى الصفرانين هي counter-stain قلنا الخطوة الأولى
+
+275
+00:20:43,270 --> 00:20:45,850
+اللي هي السمير الـ preparation الخطوة الثانية are
+
+276
+00:20:45,850 --> 00:20:48,970
+drying heat fixation الـ primary stain الملاكايت
+
+277
+00:20:48,970 --> 00:20:53,670
+جرين اللي هي water soluble لكن سأعرضها لـ steam عشان
+
+278
+00:20:53,670 --> 00:20:57,230
+يزيد اختراق الملاكايت جرين للـ spore أو لـ
+
+279
+00:20:57,230 --> 00:21:01,430
+الكيراتين سأعمل cooling mordant بعدها الـ
+
+280
+00:21:01,430 --> 00:21:05,730
+decolorizer بالـ water وعرفنا السبب لأن الصبغة
+
+281
+00:21:05,730 --> 00:21:10,030
+الملاكايت جرين water soluble سأعمل counterstain اللي
+
+282
+00:21:10,030 --> 00:21:19,070
+هي ستكون الصفرانين. هذه بالنسبة للخطوات بالنسبة
+
+283
+00:21:19,070 --> 00:21:23,330
+لهذه الصورة، سأعمل مقارنة بين spore forming bacteria
+
+284
+00:21:23,330 --> 00:21:25,870
+و non spore forming bacteria. الـ spore forming
+
+285
+00:21:25,870 --> 00:21:30,390
+bacteria البكتيريا فيها جواها endospore لكن الـ non
+
+286
+00:21:30,390 --> 00:21:35,320
+spore forming لا تحتوي على spore سواء كانت spore
+
+287
+00:21:35,320 --> 00:21:38,600
+forming أو non spore forming. البكتيريا والـ spore
+
+288
+00:21:38,600 --> 00:21:45,280
+سيكونوا قبل الصباغة transparent سأضيف
+
+289
+00:21:45,280 --> 00:21:48,040
+الـ primary stain الملاكايت الـ green أكيد البكتيريا
+
+290
+00:21:48,040 --> 00:21:51,620
+بكل سهولة ستأخذ الصبغة لأنها basic stain لكن الـ
+
+291
+00:21:51,620 --> 00:21:55,220
+spore قلنا فيه كيراتين صعب أن يُصبغ، عشان هيك
+
+292
+00:21:55,220 --> 00:22:00,620
+نعرضها لـ steam. هذا الـ steam سيعمل أو سيساعد على
+
+293
+00:22:00,620 --> 00:22:05,120
+اختراق الملاكايت الـ green للـ spore سأعمل cooling سيعمل
+
+294
+00:22:05,120 --> 00:22:08,100
+renaturation للروابط اللي انكسرت في الكيراتين اللي
+
+295
+00:22:08,100 --> 00:22:12,880
+موجود في الـ spore بعدها سأغسل بالـ water
+
+296
+00:22:12,880 --> 00:22:17,080
+decolorization with water. نقول اللون سيروح من
+
+297
+00:22:17,080 --> 00:22:21,500
+أين؟ from the cell  باستثناء الـ spore. سؤال مهم ليش
+
+298
+00:22:21,500 --> 00:22:25,450
+اللون راح من البكتيريا وضل في الـ spore ليش الـ
+
+299
+00:22:25,450 --> 00:22:29,390
+spore ضل بالـ spore لأن الـ spore فيه مادة الكيراتين
+
+300
+00:22:29,390 --> 00:22:32,790
+احنا كسرنا الروابط من خلال الـ steam، أعادنا الروابط
+
+301
+00:22:32,790 --> 00:22:37,190
+من خلال الـ cooling ثبتت الصبغة عشان هيك مهم جدا الـ
+
+302
+00:22:37,190 --> 00:22:41,070
+cooling. لو أنا ما عملت cooling مدة كافية ممكن اللون
+
+303
+00:22:41,070 --> 00:22:45,610
+سيروح حتى من الـ spore ويطلع عندي false result
+
+304
+00:22:45,610 --> 00:22:49,410
+الخلية البكتيريا راح منها اللون لأن الـ cell wall
+
+305
+00:22:49,410 --> 00:22:54,310
+تبعها ضعف من الـ steam من الحرارة وهي اصلا ما عندها
+
+306
+00:22:54,310 --> 00:22:58,490
+كيراتين. تقدر تحتفظ باللون. فلما أنا غسلت الملاكايت
+
+307
+00:22:58,490 --> 00:23:02,210
+جرين الصبغة بالـ water سهل أنها تروح لأنها water
+
+308
+00:23:02,210 --> 00:23:06,590
+soluble. بعدها سأضيف الـ counterstain الصفرانين أكيد
+
+309
+00:23:06,590 --> 00:23:09,990
+ستأخذ اللون. اللي نزال منه اللون يعني البكتيريا هي
+
+310
+00:23:09,990 --> 00:23:14,070
+اللي ستأخذ اللون. الـ spore سيظل لونها أخضر. يبقى اذا
+
+311
+00:23:14,070 --> 00:23:17,170
+كانت الخلية كلها لونها أحمر هي non spore forming
+
+312
+00:23:17,170 --> 00:23:21,370
+اذا كانت الخلية لونها أحمر والـ spore أخضر يبقى هي
+
+313
+00:23:21,370 --> 00:23:26,560
+spore forming. في دي ملاحظة اللي قلناها أن الـ
+
+314
+00:23:26,560 --> 00:23:30,280
+water في الـ spore staining اعتبرته كـ decolorizer
+
+315
+00:23:30,280 --> 00:23:35,100
+لأن الملاكايت green يحتوي على copper salt is
+
+316
+00:23:35,100 --> 00:23:38,260
+water soluble. احنا قلنا الملاكايت green هي water
+
+317
+00:23:38,260 --> 00:23:41,360
+soluble. بالتحديد ايش المكون اللي في الملاكايت
+
+318
+00:23:41,360 --> 00:23:45,680
+green اللي هو water soluble؟ الـ copper salt بالتالي
+
+319
+00:23:45,680 --> 00:23:50,100
+سهل أنه يزيل الصبغة عن الـ water. سهل أنه يزيل
+
+320
+00:23:50,100 --> 00:23:55,890
+الصبغة عن الخلية البكتيرية. vegetative cell have
+
+321
+00:23:55,890 --> 00:23:59,470
+been destroyed by heat because of this reason
+
+322
+00:23:59,470 --> 00:24:02,870
+the malachite green runs easily from the
+
+323
+00:24:02,870 --> 00:24:05,270
+vegetative cell. بقول لي أن الـ vegetative cell
+
+324
+00:24:05,270 --> 00:24:08,470
+البكتيريا تدمرت بواسطة الـ heat أو الـ steam
+
+325
+00:24:08,470 --> 00:24:13,510
+بالتالي سهل على الصبغة أنها تروح من الـ vegetative
+
+326
+00:24:13,510 --> 00:24:17,950
+cell بدون الحاجة لأي مادة كيميائية لأن الـ
+
+327
+00:24:17,950 --> 00:24:24,650
+malachite green هي water soluble. هذه الخطوات  مع
+
+328
+00:24:24,650 --> 00:24:30,310
+الصور نفس اللي شرحناها ما فيه أي اضافات. هاي
+
+329
+00:24:30,310 --> 00:24:33,870
+بالنسبة للـ result النتيجة. الـ vegetatives اللي
+
+330
+00:24:33,870 --> 00:24:37,470
+ملونة باللون الـ pink لكن الـ endospore متلون باللون
+
+331
+00:24:37,470 --> 00:24:41,690
+الأخضر. في عندي endospore متلونة باللون
+
+332
+00:24:41,690 --> 00:24:44,570
+الأخضر وعندي الـ vegetatives اللي متلونة باللون الـ
+
+333
+00:24:44,570 --> 00:24:48,530
+pink. في هذه الصورة في عندي endospore متلونة باللون
+
+334
+00:24:48,530 --> 00:24:52,210
+الأخضر والخلية متلونة باللون الأحمر وفي عندي
+
+335
+00:24:52,210 --> 00:24:56,770
+exospore متلونة باللون الأخضر. هيك بنكون خلصنا أول
+
+336
+00:24:56,770 --> 00:25:00,670
+special component اللي هو الـ endospore. تعرفنا على
+
+337
+00:25:00,670 --> 00:25:02,670
+الـ endospore staining
diff --git a/PL9fwy3NUQKwZWgAb_V1ATlRRsH-6i3cPC/zA7UNireE5w_postprocess.srt b/PL9fwy3NUQKwZWgAb_V1ATlRRsH-6i3cPC/zA7UNireE5w_postprocess.srt
new file mode 100644
index 0000000000000000000000000000000000000000..0dea63cf7d24086a7c1370abe01605ca4fd5caae
--- /dev/null
+++ b/PL9fwy3NUQKwZWgAb_V1ATlRRsH-6i3cPC/zA7UNireE5w_postprocess.srt
@@ -0,0 +1,1348 @@
+1
+00:00:00,520 --> 00:00:04,220
+السلام عليكم ورحمة الله وبركاته يعطيكم العافية
+
+2
+00:00:04,220 --> 00:00:08,160
+جميعا نستكمل معاكم محاضرات مساق الـ Microbiology
+
+3
+00:00:08,160 --> 00:00:12,060
+العملي في المحاضرة السابقة اتعرفنا على الستان
+
+4
+00:00:12,060 --> 00:00:15,700
+قسمنا الصبغات حسب ال function ل simple stand،
+
+5
+00:00:15,700 --> 00:00:18,840
+differential stand و special stand في هذا اللقاء
+
+6
+00:00:18,840 --> 00:00:21,720
+هنتناول أخر نوع من أنواع الصبغات اللي هو ال
+
+7
+00:00:21,720 --> 00:00:26,010
+special standالـ specialists قلنا تستخدم to view
+
+8
+00:00:26,010 --> 00:00:29,770
+specific part of bacteria specific part يعني
+
+9
+00:00:29,770 --> 00:00:35,050
+special component هذه المكونات عدم وجودها لا يؤثر
+
+10
+00:00:35,050 --> 00:00:39,000
+على الخلية البكتيريةفي المقابل في عندي essential
+
+11
+00:00:39,000 --> 00:00:44,060
+component هي مكونات عمق دم وجودها هيأثر على الخلية
+
+12
+00:00:44,060 --> 00:00:49,480
+البكتيرية زي مين essential component سلمبران سلوال
+
+13
+00:00:49,480 --> 00:00:53,780
+الـcytoplasm النيوكلياس كلها هي تعتبر essential
+
+14
+00:00:53,780 --> 00:00:58,460
+component الخلية البكتيرية ما بتقدر تستغني عنها
+
+15
+00:00:58,460 --> 00:01:03,600
+لكن بالنسبة لال special component من الأمثلة عندي
+
+16
+00:01:03,600 --> 00:01:08,970
+الـSpar الكابسول الفلاجلةهنتعرف في هذه المحاضرة
+
+17
+00:01:08,970 --> 00:01:13,090
+كيف بدي أنا أصبغ ال special component عندي ال
+
+18
+00:01:13,090 --> 00:01:16,470
+Indospore stain، ال capsule stain و ال flagella
+
+19
+00:01:16,470 --> 00:01:20,870
+stain أول special component هنتكلم عنه اللي هو ال
+
+20
+00:01:20,870 --> 00:01:26,510
+spar Indospore staining صباغة ال Indospore the
+
+21
+00:01:26,510 --> 00:01:30,270
+name Indospore is suggestive of a spar or seed
+
+22
+00:01:30,270 --> 00:01:35,440
+like form بقوللي أنه شكل البوغ بيشبه البذورفي جملة
+
+23
+00:01:35,440 --> 00:01:39,640
+اكتير سمعناها ان الأبواق في البكتيريا تستخدم
+
+24
+00:01:39,640 --> 00:01:43,800
+للحماية for protection لكن في الفطريات تستخدم
+
+25
+00:01:43,800 --> 00:01:50,120
+للتكاثر كالبذور في النباتات Indo means within
+
+26
+00:01:50,120 --> 00:01:54,560
+بقولنا طول ما الاسبار داخل الخلية بسميه Indo-espar
+
+27
+00:01:54,560 --> 00:01:59,360
+بعد كده ممكن تبدأ تتحلل البكتيريا ويطلع الاسبار
+
+28
+00:01:59,360 --> 00:02:04,460
+واسميه Re-espar لكن طول ما هو داخل الخلية بسميه
+
+29
+00:02:04,720 --> 00:02:09,140
+Indospore بقولنا ان البكتيريا لما تكون في حالتها
+
+30
+00:02:09,140 --> 00:02:13,740
+الطبيعية و تكون الظروف مهيئة لانه مو التكاثر بسمي
+
+31
+00:02:13,740 --> 00:02:18,280
+الخلية vegetative cell خلية تكاثرية هي ال
+
+32
+00:02:18,280 --> 00:02:22,440
+vegetative cell في حال اتغيرت الظروف و أصبحت غير
+
+33
+00:02:22,440 --> 00:02:28,300
+مناسبة لانه مو البكتيريا بتقوم بتكوين الأبواغ ال
+
+34
+00:02:28,300 --> 00:02:32,460
+sport كنوع من أنواع الحماية من العوامل الخارجية في
+
+35
+00:02:32,460 --> 00:02:37,520
+هذه الحالةلما تتحول ال vegetative cell ل spar بسمي
+
+36
+00:02:37,520 --> 00:02:42,580
+العملية sparulation لكن لو اتحسنت الظروف و تم
+
+37
+00:02:42,580 --> 00:02:47,600
+تزويدها بمغضيات راح ترجع ال spar ل vegetative cell
+
+38
+00:02:47,600 --> 00:02:52,790
+بعملية راح ترجع ال sparلـ vegetative cell بعملية
+
+39
+00:02:52,790 --> 00:02:57,050
+اسمها ال germination يبقى ال speculation هي عملية
+
+40
+00:02:57,050 --> 00:03:00,270
+تحويل ال vegetative cell ل endospar لو اتحسنت
+
+41
+00:03:00,270 --> 00:03:03,530
+الظروف هترجع ال endospar ل vegetative cell في
+
+42
+00:03:03,530 --> 00:03:09,350
+عملية ال germination ال endospar يعتبر resting
+
+43
+00:03:09,350 --> 00:03:15,600
+structure يعني resting يعني خامل يعني non activeby
+
+44
+00:03:15,600 --> 00:03:19,240
+some bacteria قال لي some مش all لإنه قلنا إن
+
+45
+00:03:19,240 --> 00:03:22,800
+الاسبار عبارة عن special component مش كل أنواع
+
+46
+00:03:22,800 --> 00:03:25,700
+البكتيريا بتكونه، في بكتيريا عندها القدرة على
+
+47
+00:03:25,700 --> 00:03:28,980
+تكوين الاسبار، spar farming bacteria وفي عند
+
+48
+00:03:28,980 --> 00:03:33,660
+بكتيريا ما عندها القدرة إنها تكون الاسبار، non
+
+49
+00:03:33,660 --> 00:03:38,940
+spar farming bacteriafor survival during adverse
+
+50
+00:03:38,940 --> 00:03:43,180
+environmental conditions بقولك تستخدم للحماية و
+
+51
+00:03:43,180 --> 00:03:47,920
+للبقاء survival اللي هي البقاء في الظروف البيئية
+
+52
+00:03:47,920 --> 00:03:52,020
+الصعبة على سبيل المثال في nutrient limitation في
+
+53
+00:03:52,020 --> 00:03:57,300
+حال نقص الأغذية or extreme environmentفي حال كان
+
+54
+00:03:57,300 --> 00:04:02,060
+عندي ظروف بيئية صعبة زي الجفاف الـ Spore are
+
+55
+00:04:02,060 --> 00:04:05,860
+resistant to heat and chemical بقوللي ان الـ Spore
+
+56
+00:04:05,860 --> 00:04:10,980
+مقاوم للحرارة و للمواد الكيميائية ليش؟ because of
+
+57
+00:04:10,980 --> 00:04:14,260
+a tough outer covering made of the protein keratin
+
+58
+00:04:14,260 --> 00:04:18,340
+بقولنا لأن هو محاط ببروتين اسمه الـ keratin يبقى
+
+59
+00:04:18,340 --> 00:04:22,390
+هذا مصطلح الـ keratin مهميبقى وجود الكيراتين هو
+
+60
+00:04:22,390 --> 00:04:25,970
+اللي بيخلّي الـ Spore مقاوم للحرارة وللمواد
+
+61
+00:04:25,970 --> 00:04:30,090
+الكيميائية من ضمن المواد الكيميائية الـ Stain
+
+62
+00:04:30,090 --> 00:04:34,730
+الصبغات يبقى the keratin also resist staining يبقى
+
+63
+00:04:34,730 --> 00:04:39,330
+الكيراتين هيكون مقاوم لل Staining للصباغة ال Spore
+
+64
+00:04:39,330 --> 00:04:44,630
+هيكون مقاومة للصباغة so extreme measuremust be
+
+65
+00:04:44,630 --> 00:04:48,050
+taken to stand a spar عشان هيك لازم انا اخد بعين
+
+66
+00:04:48,050 --> 00:04:54,530
+الاعتبار هذا الاشي لما بدي انا اصبغ ليه spar في
+
+67
+00:04:54,530 --> 00:04:59,350
+هاي ال slide هنتعرف على خصائص ال spar ال endospar
+
+68
+00:04:59,350 --> 00:05:03,930
+can remain dormant indefinitely not reproductive
+
+69
+00:05:03,930 --> 00:05:09,590
+بقوللي انه ال endospar بيضلوا خامل لمدة الحياة لكن
+
+70
+00:05:09,590 --> 00:05:13,170
+لو اتحسنت الظروف but germinate quickly when
+
+71
+00:05:13,700 --> 00:05:17,120
+appropriate trigger is applied لو اتحسنت الظروف
+
+72
+00:05:17,120 --> 00:05:21,220
+ممكن انه يسله جرمن عشان يتحول ال sport ل
+
+73
+00:05:21,220 --> 00:05:26,300
+vegetative cell لكن بيظلوا خامل فترة طويلة جدا مش
+
+74
+00:05:26,300 --> 00:05:31,480
+هيتكاثر non reproductive هو inactive قولنا ان هو
+
+75
+00:05:31,480 --> 00:05:35,740
+خامل dehydrated يعني منزوع منه الميه stable for
+
+76
+00:05:35,740 --> 00:05:40,560
+years قولناإنه بيظلوا استابل لسنوات عديدة وطويلة
+
+77
+00:05:40,560 --> 00:05:45,280
+ممكن في حالة حسنة الظروف حتى لو بعد مدة طويلة عنده
+
+78
+00:05:45,280 --> 00:05:49,260
+القدرة to germinate ويتحول ل vegetative cell
+
+79
+00:05:49,260 --> 00:05:53,700
+Indosperm differs significantly from the
+
+80
+00:05:53,700 --> 00:05:57,580
+vegetative or normally functioning cell بقولي إن
+
+81
+00:05:57,580 --> 00:06:01,100
+ال Indosperm بيختلف كليا عن ال vegetative cell
+
+82
+00:06:01,100 --> 00:06:06,000
+قلنا لinactive، dormant، لكن الـ vegetative cell
+
+83
+00:06:06,000 --> 00:06:09,840
+reproductive، تكاثرية الـ spar is stable for
+
+84
+00:06:09,840 --> 00:06:14,240
+years، لكن ال vegetative cell مش stable، هتمر بالـ
+
+85
+00:06:14,240 --> 00:06:19,640
+death phase، هتموت، هتمر في طور الموت بكتيريا can
+
+86
+00:06:19,640 --> 00:06:24,080
+form endospars in approximately six to eight hours
+
+87
+00:06:24,080 --> 00:06:27,980
+after being exposed to adverse conditionsبقول لي
+
+88
+00:06:27,980 --> 00:06:31,800
+البكتيريا بتقدر اتكون لـ Spore طبعا في حال أصبحت
+
+89
+00:06:31,800 --> 00:06:37,100
+الظروف غير مناسبة للنمو تقريبا بعد 6 ل 8 ساعات من
+
+90
+00:06:37,100 --> 00:06:41,000
+تعرضها لهذه الظروف السيئة يعني عملية الـ
+
+91
+00:06:41,000 --> 00:06:45,340
+Sporulation تحول ال vegetative cell لIndospore
+
+92
+00:06:45,340 --> 00:06:51,440
+بتتم بعد 6 ل 8 ساعات من تعرضها لظروف germinate
+
+93
+00:06:51,440 --> 00:06:57,130
+into a vegetative cell with 1.5 power لكن بقولنافي
+
+94
+00:06:57,130 --> 00:07:02,150
+حال تحسنت الظروف الـ spar هتتحول لـvegetative cell
+
+95
+00:07:02,150 --> 00:07:06,270
+خلال ساعة و نص يبقى عملية ال germination بتتم بعد
+
+96
+00:07:06,270 --> 00:07:10,390
+ساعة و نص من تحسن الظروف عشان هيك في أول جملة
+
+97
+00:07:10,390 --> 00:07:14,850
+قاللي انه germinate quickly عملية ال germination
+
+98
+00:07:14,850 --> 00:07:18,410
+بتسير بسرعة بعد ساعة و نص من تحسن الظروف لكن ال
+
+99
+00:07:18,410 --> 00:07:23,050
+sparulation بتتسير بعد 6 ل 8 ساعات من تعرضها
+
+100
+00:07:23,050 --> 00:07:27,680
+للظروف السيئة يعني ال germination بتكونأسرع من الـ
+
+101
+00:07:27,680 --> 00:07:31,960
+Sparulation Spar
+
+102
+00:07:31,960 --> 00:07:36,580
+formed by gram positive bacteria و احنا قلنا انه
+
+103
+00:07:36,580 --> 00:07:40,180
+مش كل البكتيريا بتكون لـ Spar أشهر البكتيريا اللي
+
+104
+00:07:40,180 --> 00:07:45,160
+بتكون لـ Spar هي البصلص و الكلستريديوم البصلص و
+
+105
+00:07:45,160 --> 00:07:49,670
+الكلستريديوم هدول gram positive bacteriaالـ Gram
+
+106
+00:07:49,670 --> 00:07:53,750
+-positive Bacilli الشاب تبعهم Bacilli تمام
+
+107
+00:07:53,750 --> 00:07:56,190
+البكتيريا لـ Spar farming بقولنا
+
+108
+00:07:56,190 --> 00:07:59,730
+البسيلسوليكلوستريديوم هي Gram-positive بكتيريا هذا
+
+109
+00:07:59,730 --> 00:08:03,090
+لا يعني أن كل Gram-positive هي Spar farming في
+
+110
+00:08:03,090 --> 00:08:06,310
+عندي بكتيريا Gram-positive لكن لا Spar farming
+
+111
+00:08:06,310 --> 00:08:10,570
+بدنا نحكي عن أشهر البكتيريا لـ Spar farming هنتكلم
+
+112
+00:08:10,570 --> 00:08:15,440
+عنهم بشكل مختصرأول بكتيريا الـ Bacillus anthracis
+
+113
+00:08:15,440 --> 00:08:21,000
+اللي بتسبب الـ Anthrax الجمرة الخبيثة الإصابة بهذه
+
+114
+00:08:21,000 --> 00:08:24,720
+البكتيريا ممكن تكون عن طريق ال injection أو ال
+
+115
+00:08:24,720 --> 00:08:27,780
+inhalation الاستنشاق استنشاق الـ Bacillus
+
+116
+00:08:27,780 --> 00:08:32,700
+anthracis sport الاستنشاق كانوا يستخدموها في
+
+117
+00:08:32,700 --> 00:08:36,590
+الحروب البيولوجيةبروحوا بيجيبوا ضرف و بيحطوا فيه
+
+118
+00:08:36,590 --> 00:08:39,690
+الـ spar تبع البكتيريا و بيروحوا بيبعتوها لقى
+
+119
+00:08:39,690 --> 00:08:43,950
+الدولة المعادية فلما يصل الضرف بيفتحه بيشمه
+
+120
+00:08:43,950 --> 00:08:49,250
+بيستنشق فبالتالي بيصاب بي ال anthrax بي الجمرة
+
+121
+00:08:49,250 --> 00:08:53,530
+الخبيثة بالنسبة لكلوستريديوم الكلوستريديوم في عندي
+
+122
+00:08:53,530 --> 00:08:57,590
+three species بيسببوا أمراض للإنسان أول واحد
+
+123
+00:08:57,590 --> 00:09:01,940
+الكلوستريديوم Botulinum اللي بيسبباللي بيسبب الـ
+
+124
+00:09:01,940 --> 00:09:06,220
+botulism اللي هو الـ food poisoning بيسبب هذا
+
+125
+00:09:06,220 --> 00:09:09,820
+بيصير في عيني انا لو شخص انصاب الكلستريديوم
+
+126
+00:09:09,820 --> 00:09:13,920
+بوتولينيوم بيصير في عينيه شلل ارتخائي عشان هيك
+
+127
+00:09:13,920 --> 00:09:19,340
+بيتم استخدامه في عمليات التجميل ال Botox بيتم حقد
+
+128
+00:09:19,340 --> 00:09:24,230
+منطقة مثلا حول العين عشان يصير في عيني ارتخاءفي
+
+129
+00:09:24,230 --> 00:09:28,170
+عندى نوع اخر من الـ Clostridium سبيشيز اخر من ال
+
+130
+00:09:28,170 --> 00:09:32,090
+Clostridium Clostridium Perifringes اللى بتسبب gas
+
+131
+00:09:32,090 --> 00:09:36,750
+gangrene اللى هى نقص وصول ال oxygen للانسجار
+
+132
+00:09:36,750 --> 00:09:42,190
+فبالتالي بيصير العضو لونه أسود Clostridium Tetani
+
+133
+00:09:42,190 --> 00:09:45,890
+اللى بتسبب ال tetanus او له اسم تانى ال fixed
+
+134
+00:09:45,890 --> 00:09:49,950
+smile او الكزاز بيكون ضغط على أسنانه بيصير عنده
+
+135
+00:09:49,950 --> 00:09:53,630
+شلل انقباضىيبقى الـ Clostridium تيتاني بيكون فيه
+
+136
+00:09:53,630 --> 00:09:57,210
+عندى شلل انقباضي لكن الـ Clostridium Botulinum
+
+137
+00:09:57,210 --> 00:10:02,710
+بيصير فيه عندى شلل ارتخالي هاي بالنسبة لأشهر
+
+138
+00:10:02,710 --> 00:10:06,130
+البكتيريا الـ Spar Forming هتتعرفوا أكتر عن كل
+
+139
+00:10:06,130 --> 00:10:09,530
+بكتيريا عن الـ pathogenesis طريقة إحدافها للمرض
+
+140
+00:10:09,530 --> 00:10:14,170
+لكن المطلوب تعرفوا أن هدول هم Spar Forming المطلوب
+
+141
+00:10:14,170 --> 00:10:23,650
+نعرف شو اسم المرض اللي بتسببه كل بكتيرياIndospore
+
+142
+00:10:23,650 --> 00:10:27,230
+location موقع الاندوسبورت position of the
+
+143
+00:10:27,230 --> 00:10:31,710
+endospore differs among bacterial species and is
+
+144
+00:10:31,710 --> 00:10:36,590
+useful in identification بقوللي أن موقع
+
+145
+00:10:36,590 --> 00:10:40,010
+الاندوسبورت بختلف من species لآخر ممكن يكون
+
+146
+00:10:40,010 --> 00:10:43,950
+central endospore يقع في المنتصف ممكن يكون sub
+
+147
+00:10:43,950 --> 00:10:47,450
+terminal قريب من الطرف ممكن يكون terminal
+
+148
+00:10:47,450 --> 00:10:51,670
+endosporeمن الأمثلة على الـ «Central» البصيلة
+
+149
+00:10:51,670 --> 00:10:55,090
+«Ceres» والبصيلة «Anthrax» «Terminal»
+
+150
+00:10:55,090 --> 00:10:58,470
+«Eclostridium» «Titanium» «Subterminal» البصيلة
+
+151
+00:10:58,470 --> 00:11:01,670
+«Subtules» يبقى في عندي إضافات البصيلة «Ceres»
+
+152
+00:11:01,670 --> 00:11:06,110
+والبصيلة «Subtules» أنا بعتبرهم «Spar Farming
+
+153
+00:11:06,110 --> 00:11:10,570
+Bacteria» احنا قلنا انه لـ «Gram Stain» هي أول
+
+154
+00:11:10,570 --> 00:11:14,790
+خطوة من خطوات تعريف البكتيريا لكن ما بقدر من خلال
+
+155
+00:11:14,790 --> 00:11:19,410
+«Gram Stain» فقط أتعرف على البكتيريابقول لي من
+
+156
+00:11:19,410 --> 00:11:22,390
+خلال الـ IndoSpar Stain أو الـ IndoSpar Location
+
+157
+00:11:22,390 --> 00:11:26,630
+ممكن يساعد في التعريف، كيف؟ على سبيل المثال، كأن
+
+158
+00:11:26,630 --> 00:11:31,890
+عندي بكتيريا X، بدي أنا أتعرف عليها أول خطوة عشان
+
+159
+00:11:31,890 --> 00:11:36,030
+أعمل تعريف، بعمل لـ Gram Stain عملت Gram Stain،
+
+160
+00:11:36,030 --> 00:11:39,790
+طلعت Gram Positive Bustle Life طيب، أنا أعمل لـ
+
+161
+00:11:39,790 --> 00:11:42,450
+Gram Positive Bustle Life يعني مجموعة كبيرة من
+
+162
+00:11:42,450 --> 00:11:45,650
+البكتيريا، بعرف أن في مجموعة ممكن تكون Spar
+
+163
+00:11:45,650 --> 00:11:49,580
+Formingوفيه ممكن يكون منها non-spar farming فروح
+
+164
+00:11:49,580 --> 00:11:56,020
+اتعملت الـ endospar stem طلعت من ال endospar stem
+
+165
+00:11:56,020 --> 00:12:00,220
+البكتيريا هي spar farming يبقى هستثنى كل البكتيريا
+
+166
+00:12:00,220 --> 00:12:04,680
+non-spar farming من ال gram positive bacilli هروح
+
+167
+00:12:04,680 --> 00:12:08,540
+افكر ب spar farming من ال gram positive bacilli
+
+168
+00:12:08,540 --> 00:12:11,860
+انا بعرف من ال spar farming ال gram positive
+
+169
+00:12:11,860 --> 00:12:17,160
+bacilli اللي هي البصلص والكلستريديومعشان افرق بين
+
+170
+00:12:17,160 --> 00:12:20,320
+البصلص و الـ Clostridium في فحص كيميا ايه هنتعرف
+
+171
+00:12:20,320 --> 00:12:24,740
+عليه في المعامل اللي جاي طيب انا هذا الفحص عملته
+
+172
+00:12:24,740 --> 00:12:27,860
+كانت النتيجة ان هاي البكتيريا بصلص طب فيه
+
+173
+00:12:27,860 --> 00:12:31,700
+اندوسبيشيز من البصلص كيف بدي اعرف اي species هاي
+
+174
+00:12:31,700 --> 00:12:35,560
+البكتيريا؟ بقوللي من خلال موقع الIndospar
+
+175
+00:12:35,560 --> 00:12:39,720
+الIndospar location انا سبقت كان الIndospar
+
+176
+00:12:39,720 --> 00:12:43,940
+location اللي هو sub terminal يبقى البكتيريا هي
+
+177
+00:12:43,940 --> 00:12:48,460
+بصلصيبقى هيك أنا من خلال الـ Position of Endospore
+
+178
+00:12:48,460 --> 00:12:54,720
+ساعدني في تعريف البكتيريا Endospore
+
+179
+00:12:54,720 --> 00:12:58,200
+shape شكل الـ Endospore ممكن يكون كراوي ممكن يكون
+
+180
+00:12:58,200 --> 00:13:02,440
+بيضاوي و ممكن يكون أسطوان في عندي أشكال مختلفة
+
+181
+00:13:02,440 --> 00:13:06,180
+بالنسبة للـ Endospore size بقولي أن الـ Endospore
+
+182
+00:13:06,180 --> 00:13:10,440
+can also be larger or smaller in diameter than the
+
+183
+00:13:10,440 --> 00:13:14,560
+vegetative cell Endospore ممكن يكون حجمهأكبر أو
+
+184
+00:13:14,560 --> 00:13:20,640
+أصغر من الـ vegetative cell في أول تلت بكتيريا أول
+
+185
+00:13:20,640 --> 00:13:23,300
+تلت صور هذه الصورة الأولى والتانية والتالتة
+
+186
+00:13:23,300 --> 00:13:26,760
+مانلاحظ انه ال Indus Bar أصغر من ال vegetative
+
+187
+00:13:26,760 --> 00:13:30,360
+cell لكن في الصورة الرابعة والخامسة والسادسة ال
+
+188
+00:13:30,360 --> 00:13:34,480
+Indus Bar كان أكبر من ال vegetative cell وسبب
+
+189
+00:13:34,480 --> 00:13:38,360
+الانتفاخ لل vegetative cell في المنطقة المحيطة بال
+
+190
+00:13:38,360 --> 00:13:41,600
+Indus Bar مش في كل ال vegetative cell فقط المنطقة
+
+191
+00:13:41,600 --> 00:13:45,750
+المحيطة بال Indus Barthose that are larger in
+
+192
+00:13:45,750 --> 00:13:48,990
+diameter will produce an area of swelling in the
+
+193
+00:13:48,990 --> 00:13:52,750
+vegetative cell هيسبب لانتفاخ لال vegetative cell
+
+194
+00:13:52,750 --> 00:13:56,210
+الانتفاخ هيكون فقط في المنطقة المحيطة بالـ
+
+195
+00:13:56,210 --> 00:14:02,970
+Endospore طيب نتعرف هلأ على طريقة الصباغة الـ
+
+196
+00:14:02,970 --> 00:14:06,670
+Endospore Staining اللي هاسم تاني discover Fulton
+
+197
+00:14:06,670 --> 00:14:11,650
+Method لازم نكون عارفينأي استان إذا إلها اسم تاني
+
+198
+00:14:11,650 --> 00:14:14,710
+نكون عارفين هل ده الاسم زي الـ Acid Fast Stain كان
+
+199
+00:14:14,710 --> 00:14:19,430
+إلها الـ Zel Nelson Stain لأنه ممكن في الامتحان
+
+200
+00:14:19,430 --> 00:14:22,530
+أنا أجيب أي سؤال بيتعلق بالـ Acid Fast Stain لكن
+
+201
+00:14:22,530 --> 00:14:25,510
+ما أكتب Acid Fast Stain أكتب الاسم التاني Zel
+
+202
+00:14:25,510 --> 00:14:29,410
+Nelson Stain فتكوني أنتي عارفة إن هي هاي هي نفس
+
+203
+00:14:29,410 --> 00:14:35,690
+الـ Acid Fast Stain كأي خطوة كأي استان في أي في
+
+204
+00:14:35,690 --> 00:14:39,480
+المعمل الـ Microbiology Lab أول خطوةSmear
+
+205
+00:14:39,480 --> 00:14:42,900
+Preparation الخطوة التانية Air Drying الخطوة
+
+206
+00:14:42,900 --> 00:14:48,000
+التالتة هي Fixation وعارفين كل تفاصيل هذه الخطوات
+
+207
+00:14:48,000 --> 00:14:52,400
+اللي اترقنا اليهم في معمل ال symbol STAIN الخطوة
+
+208
+00:14:52,400 --> 00:14:56,320
+الرابعة بتكون ال STAIN قولنا هي بتختلف حسب نوع ال
+
+209
+00:14:56,320 --> 00:15:02,200
+STAIN اليوم هنتكلم على ال Endospore STAIN قولنا في
+
+210
+00:15:02,200 --> 00:15:07,520
+بداية المعمل ان ال STAIN مقاوم للصبغة نتيجة وجود
+
+211
+00:15:07,520 --> 00:15:11,680
+الكيراتينإذا بتتذكروا في الـ Acid fast stain قلنا
+
+212
+00:15:11,680 --> 00:15:15,880
+الـ Mycobacterium مقاومة أيضًا للصبغة نتيجة وجود
+
+213
+00:15:15,880 --> 00:15:20,900
+الـ wax lipid لكن أنا قدرت أخترق ال wax lipid عن
+
+214
+00:15:20,900 --> 00:15:25,020
+طريق تعريض البكتيريا للـ steam ساعد على اختراق
+
+215
+00:15:25,020 --> 00:15:28,820
+اللي هو الـ Carbolfoxin اللي كانت primarily stain
+
+216
+00:15:28,820 --> 00:15:33,420
+للـ wax lipid وقدرت أني أنا أصبغهاهان في الاندو
+
+217
+00:15:33,420 --> 00:15:37,960
+سبارك نفس الفكرة السبارك بتتكون من كيراتين صعبة
+
+218
+00:15:37,960 --> 00:15:42,800
+الصباغة فبالتالي هستخدم لستيم حتى يكسر الروابط في
+
+219
+00:15:42,800 --> 00:15:47,740
+الكيراتين وبالتالي هيسهل اختراق الصبغة لداخل
+
+220
+00:15:47,740 --> 00:15:51,060
+السبارك لكن في ال acid fast stain انا استخدمت
+
+221
+00:15:51,060 --> 00:15:53,720
+كاربولفوكسين ك primary stain مش هستخدم
+
+222
+00:15:53,720 --> 00:15:56,920
+كاربولفوكسين في الاندو سبارك كاربولفوكسين كانت
+
+223
+00:15:56,920 --> 00:16:01,590
+مميزاتها ان هيLipid soluble وهذا اللي ساعدها في
+
+224
+00:16:01,590 --> 00:16:05,990
+اختراق ال wax lipid في ال mycobacterium لكن في
+
+225
+00:16:05,990 --> 00:16:10,190
+الاسبوري ماعندي wax lipid عندي كيراتين هستخدم
+
+226
+00:16:10,190 --> 00:16:16,030
+Malachite green الملاكايت
+
+227
+00:16:16,030 --> 00:16:20,730
+جرين هي ال primary stain الملاكايت جرين بنعرف أنه
+
+228
+00:16:20,730 --> 00:16:26,850
+هي basic stainو معلومة إضافية أنها water soluble
+
+229
+00:16:26,850 --> 00:16:29,790
+يبقى نكتب هان ال primary stem
+
+230
+00:16:41,160 --> 00:16:44,480
+الـ Primary Stain الملقاقية Green وهي Water
+
+231
+00:16:44,480 --> 00:16:49,320
+Soluble يبقى عشان نحاول اننا نحفظ ونقارن ال Acid
+
+232
+00:16:49,320 --> 00:16:52,280
+Phosphate Stain استخدمت ال Primary Stain الكربو
+
+233
+00:16:52,280 --> 00:16:56,400
+الفوكسيني اللي تعتبر Lipid Soluble عشان ال wax
+
+234
+00:16:56,400 --> 00:16:59,900
+lipid الموجود في ال Mycobacterium لكن في ال Indus
+
+235
+00:16:59,900 --> 00:17:03,360
+Pore استخدمت ال Primary Stain الملقاقية Green اللي
+
+236
+00:17:03,360 --> 00:17:08,540
+هي Water Soluble ليش استخدمتها نعرف في ال slides
+
+237
+00:17:08,540 --> 00:17:12,810
+الجايةأول خطوة في الستان طبعا احنا قلنا spear
+
+238
+00:17:12,810 --> 00:17:16,050
+preparation and air drying heat fixation بعدها
+
+239
+00:17:16,050 --> 00:17:19,770
+هصبغ هجيب بيكر احط فيه ميه اخليها تغلي لما تغلي
+
+240
+00:17:19,770 --> 00:17:23,910
+هحط ال slide على طرف البيكر بغمر ال slide بال
+
+241
+00:17:23,910 --> 00:17:29,030
+primary stand الملاكايت ال green هستنى من 3 ل 5
+
+242
+00:17:29,030 --> 00:17:35,570
+دقائق و بعد ما تنتهي الخمس دقائق طبعا هستنى هخليها
+
+243
+00:17:35,570 --> 00:17:39,840
+تبردالـ steam هيعمل enhance ملاكاية إيجرين
+
+244
+00:17:39,840 --> 00:17:44,560
+بنتريشون هيساعد على اختراق الملاكاية إيجرين الصبغة
+
+245
+00:17:44,560 --> 00:17:50,100
+للكيراتين للسبوع طبعا بقولي انا لو جفت الصبغة نفس
+
+246
+00:17:50,100 --> 00:17:52,920
+في ال acid phosphate بضيف الصبغة لأنها يتكون
+
+247
+00:17:52,920 --> 00:17:57,740
+كريستال لونه أخضر بعد ما اتخلص الخمس دقائق بخليها
+
+248
+00:17:57,740 --> 00:18:02,260
+تبرد يبقى الخطوة التانية cooling هعمل cooling وهي
+
+249
+00:18:02,260 --> 00:18:06,330
+الخطوة مهمة جدا لأن لو ما خلتها تبردمدة كفاية
+
+250
+00:18:06,330 --> 00:18:11,210
+هتأثر على النتائج ال cooling قولنا act as more
+
+251
+00:18:11,210 --> 00:18:15,050
+than يبقى ال cooling هيكون act as more than
+
+252
+00:18:24,320 --> 00:18:29,160
+تمام ال cooling يعتبر ك-mordant لأنه من خلال ال ال
+
+253
+00:18:29,160 --> 00:18:32,580
+cooling هيكون ك-mordant لأنه من خلال ال heat أو ال
+
+254
+00:18:32,580 --> 00:18:36,860
+steam اتكسرت الروابط في الكيراتين قدرت الصبغة
+
+255
+00:18:36,860 --> 00:18:41,080
+الملاكاية ال green تخترق ال spar حتى أثبت
+
+256
+00:18:41,080 --> 00:18:44,760
+الملاكاية ال green في ال spar بخليها تبرد لأنه ال
+
+257
+00:18:44,760 --> 00:18:48,280
+cooling هيعمل re-naturation للروابط بالتالي الصبغة
+
+258
+00:18:48,280 --> 00:18:53,280
+هتثبتبعد ال cooling gently rinse the slide with
+
+259
+00:18:53,280 --> 00:18:56,760
+water الخطوة بعد ما احنا بستخدم ال primary stain
+
+260
+00:18:56,760 --> 00:19:01,480
+ماردان بعدين بستخدم ال decolorizer ال decolorizer
+
+261
+00:19:01,480 --> 00:19:05,460
+في ال Indosporous stain هو ال water يبقى ال
+
+262
+00:19:05,460 --> 00:19:07,220
+decolorizer هو ال water
+
+263
+00:19:22,030 --> 00:19:26,530
+الـ water هعتبره ك decalorizer طب ليش؟ احنا قلنا
+
+264
+00:19:26,530 --> 00:19:31,250
+ان ال primary stain هي الملاكايت green واتفقنا على
+
+265
+00:19:31,250 --> 00:19:35,710
+معلومة ان الملاكايت green هي water soluble يبقى
+
+266
+00:19:35,710 --> 00:19:39,810
+الميه هتزيل الملاكايت green هتزيل الصبغة بدون
+
+267
+00:19:39,810 --> 00:19:44,830
+الحاجة لأي مادة كيميائية يبقى عرفنا ليش انا اخترت
+
+268
+00:19:44,830 --> 00:19:48,890
+بالذات الملاكايت green ك primary stain لأنها water
+
+269
+00:19:48,890 --> 00:19:54,160
+solubleو احنا ما استخدمنا اي مادة كيميائية اعتبرنا
+
+270
+00:19:54,160 --> 00:19:58,440
+ال water هو ال decalryzer بعدها هضيف ال
+
+271
+00:19:58,440 --> 00:20:04,800
+counterstain للصفرانين لمدة من 1 ل 2 من 1 ل 2
+
+272
+00:20:04,800 --> 00:20:09,800
+minute بعدها هغسل بالwater هعمل air drying على ال
+
+273
+00:20:09,800 --> 00:20:12,920
+oil immersion يبقى الصفرانين هي counterstain
+
+274
+00:20:38,790 --> 00:20:43,270
+يبقى الصفرانين هي counter-stain قلنا الخطوة الأولى
+
+275
+00:20:43,270 --> 00:20:45,850
+اللى هى السمير ال preparation الخطوة التانى are
+
+276
+00:20:45,850 --> 00:20:48,970
+drying heat fixation ال primary stain الملاكايت
+
+277
+00:20:48,970 --> 00:20:53,670
+جرين اللى هى water soluble لكن هعرضها ل steam عشان
+
+278
+00:20:53,670 --> 00:20:57,230
+يزيد اختراق الملاكايت جرين ل ال spar أو ل
+
+279
+00:20:57,230 --> 00:21:01,430
+الكيراتين هعمل cooling mordant بعدها ال
+
+280
+00:21:01,430 --> 00:21:05,730
+decolorizer بى ال water وعرفنا السبب لأن الصفقة
+
+281
+00:21:05,730 --> 00:21:10,030
+الملاكايت جرين water soluble هعمل counterستان اللي
+
+282
+00:21:10,030 --> 00:21:19,070
+هي هتكون الصفرانين هذه بالنسبة للخطوات بالنسبة
+
+283
+00:21:19,070 --> 00:21:23,330
+لهذه الصورة عملني مقارنة بين Spar farming bacteria
+
+284
+00:21:23,330 --> 00:21:25,870
+و non spar farming bacteria ال Spar farming
+
+285
+00:21:25,870 --> 00:21:30,390
+bacteria البكتيريا فيه جواها endospar لكن ال non
+
+286
+00:21:30,390 --> 00:21:35,320
+spar farming ما بتحتوي على Spar سواء كانتSpar
+
+287
+00:21:35,320 --> 00:21:38,600
+farming او non spar farming البكتيريا و الـ spar
+
+288
+00:21:38,600 --> 00:21:45,280
+هيكونوا قبل الصباغة transparent هضيف
+
+289
+00:21:45,280 --> 00:21:48,040
+ال primary stain لملكات الـ green أكيد البكتيريا
+
+290
+00:21:48,040 --> 00:21:51,620
+بكل سهولة هتاخد الصبغة لأنها basic stain لكن ال
+
+291
+00:21:51,620 --> 00:21:55,220
+spar قلنا في كيراتين صعب انه ينصبخ عشان هيك
+
+292
+00:21:55,220 --> 00:22:00,620
+بنعرضها ل steam هذا ال steam هيعمل او هيساعد على
+
+293
+00:22:00,620 --> 00:22:05,120
+اختراق الملكات ال green لل sparهعمل cooling هيعمل
+
+294
+00:22:05,120 --> 00:22:08,100
+renaturation للروابط اللى اتكسرت في الكيراتين اللى
+
+295
+00:22:08,100 --> 00:22:12,880
+موجود اللى فى ال spar بعدها هغسل بالwater
+
+296
+00:22:12,880 --> 00:22:17,080
+decolorization with water بقولنا اللون هيروح من
+
+297
+00:22:17,080 --> 00:22:21,500
+وين from the cell بطنات ال spar hence سؤال مهم لش
+
+298
+00:22:21,500 --> 00:22:25,450
+اللون راح من البكتيريا وضلوا فى ال sparليش الـ
+
+299
+00:22:25,450 --> 00:22:29,390
+Lawn دلوا بالـ spar لأن الـ spar في مادة الكيراتين
+
+300
+00:22:29,390 --> 00:22:32,790
+احنا كسرنا الروابط من خلال الـ steam قعدنا الروابط
+
+301
+00:22:32,790 --> 00:22:37,190
+من خلال ال cooling تبتت الصبغة عشان هيك مهم جدا ال
+
+302
+00:22:37,190 --> 00:22:41,070
+cooling لو انا ماعملت cooling مدة كافية ممكن اللان
+
+303
+00:22:41,070 --> 00:22:45,610
+يروح حتى من ال spar و يطلع عندي false result
+
+304
+00:22:45,610 --> 00:22:49,410
+الخلية البكتيريا راح منها اللان لأن ال steel wall
+
+305
+00:22:49,410 --> 00:22:54,310
+تبعها ضعف من ال steam من الحرارةوهى اصلا ما عندها
+
+306
+00:22:54,310 --> 00:22:58,490
+كيراتين تقدر تحتفظ باللون فلما انا غسلت الملاكايت
+
+307
+00:22:58,490 --> 00:23:02,210
+جرين الصبغة بالواتر سهل انها تروح لأنها water
+
+308
+00:23:02,210 --> 00:23:06,590
+soluble بعدها هضيف ال counterstain الصفرانين اكيد
+
+309
+00:23:06,590 --> 00:23:09,990
+هتاخد اللون اللى نزال منها اللون يعني البكتيرى هي
+
+310
+00:23:09,990 --> 00:23:14,070
+اللى هتاخد اللون الـ spar هيظل لونها أخضر يبقى اذا
+
+311
+00:23:14,070 --> 00:23:17,170
+كانت الخلية كلها لونها أحمر هي non spar farming
+
+312
+00:23:17,170 --> 00:23:21,370
+اذا كانت الخلية لونها أحمر والـ spar أخضر يبقى هي
+
+313
+00:23:21,370 --> 00:23:26,560
+spar farmingفين دي ملاحظة اللي قلناها انه الـ
+
+314
+00:23:26,560 --> 00:23:30,280
+water في الـ sparse tanning اعتبرته ك decalorizer
+
+315
+00:23:30,280 --> 00:23:35,100
+لأنه الملاكايت green بيحتوي على copper salt is
+
+316
+00:23:35,100 --> 00:23:38,260
+water soluble احنا قلنا الملاكايت green هي water
+
+317
+00:23:38,260 --> 00:23:41,360
+soluble بالتحديد ايش المكون اللي في الملاكايت
+
+318
+00:23:41,360 --> 00:23:45,680
+green اللي هو water soluble ال copper salt بالتالي
+
+319
+00:23:45,680 --> 00:23:50,100
+سهل انه يزيل الصبغة عن ال water سهل انه يزيل
+
+320
+00:23:50,100 --> 00:23:55,890
+الصبغة عن الخلية البكتيريةvegetative cell have
+
+321
+00:23:55,890 --> 00:23:59,470
+been distributed by heat because of this reason
+
+322
+00:23:59,470 --> 00:24:02,870
+the malachite green runs easily from the
+
+323
+00:24:02,870 --> 00:24:05,270
+vegetative cell بقول لي أن ال vegetative cell
+
+324
+00:24:05,270 --> 00:24:08,470
+البكتيريا اتدمرت بواسطة ال heat أو ال steam
+
+325
+00:24:08,470 --> 00:24:13,510
+بالتالي سهل على الصبغة أنها تروح من ال vegetative
+
+326
+00:24:13,510 --> 00:24:17,950
+cell بدون الحاجة لأي مادة كيميائية لأن ال
+
+327
+00:24:17,950 --> 00:24:24,650
+malachite green هي water solubleهذه الخطوات بسوق
+
+328
+00:24:24,650 --> 00:24:30,310
+بيه الصور نفس اللي شرحناها مافي اي اضافات هاي
+
+329
+00:24:30,310 --> 00:24:33,870
+بالنسبة لل result النتيجة ال vegetatives اللي
+
+330
+00:24:33,870 --> 00:24:37,470
+ملونة باللون ال pink لكن ال endospar متلون باللون
+
+331
+00:24:37,470 --> 00:24:41,690
+الأخضر فى عندي hand freeze bar متلونة باللون
+
+332
+00:24:41,690 --> 00:24:44,570
+الأخضر وعندي ال vegetatives اللي متلونة باللون ال
+
+333
+00:24:44,570 --> 00:24:48,530
+pinkفي هذه الصورة في عندي Indospar متلونة باللون
+
+334
+00:24:48,530 --> 00:24:52,210
+الأخضر والسل متلونة باللون الأحمر وفي عندي
+
+335
+00:24:52,210 --> 00:24:56,770
+Efrespar متلونة باللون الأخضر هيك بنكون خلصنا أول
+
+336
+00:24:56,770 --> 00:25:00,670
+special component اللي هو ال Indospar تعرفنا على
+
+337
+00:25:00,670 --> 00:25:02,670
+ال Indospar Stemming
+
diff --git a/PL9fwy3NUQKwZWgAb_V1ATlRRsH-6i3cPC/zA7UNireE5w_raw.json b/PL9fwy3NUQKwZWgAb_V1ATlRRsH-6i3cPC/zA7UNireE5w_raw.json
new file mode 100644
index 0000000000000000000000000000000000000000..8fc637da357ba97098df4eeca672521c30f966a3
--- /dev/null
+++ b/PL9fwy3NUQKwZWgAb_V1ATlRRsH-6i3cPC/zA7UNireE5w_raw.json
@@ -0,0 +1 @@
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"text": "من الأمثلة على الـ «Central» البصيلة «Ceres» والبصيلة «Anthrax» «Terminal» «Eclostridium» «Titanium» «Subterminal» البصيلة «Subtules» يبقى في عندي إضافات البصيلة «Ceres» والبصيلة «Subtules» أنا بعتبرهم «Spar Farming Bacteria» احنا قلنا انه لـ «Gram Stain» هي أول خطوة من خطوات تعريف البكتيريا لكن ما بقدر من خلال «Gram Stain» فقط أتعرف على البكتيريا", "tokens": [27842, 16247, 2304, 12984, 37977, 15844, 2423, 39184, 4657, 34, 317, 2155, 5933, 29739, 9381, 1829, 37977, 4657, 34, 9931, 5933, 16070, 3555, 9381, 1829, 37977, 4657, 7828, 392, 424, 87, 5933, 4657, 51, 966, 2071, 5933, 4657, 36, 3474, 555, 8558, 2197, 5933, 4657, 51, 9670, 2197, 5933, 4657, 39582, 7039, 2071, 5933, 29739, 9381, 1829, 37977, 4657, 39582, 83, 3473, 5933, 7251, 3555, 4587, 7578, 8978, 18871, 16254, 11933, 11242, 31845, 9307, 29739, 9381, 1829, 37977, 4657, 34, 9931, 5933, 16070, 3555, 9381, 1829, 37977, 4657, 39582, 83, 3473, 5933, 41850, 4724, 34268, 26890, 16095, 4657, 50, 2181, 9067, 2810, 363, 14125, 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"temperature": 1.0}, {"id": 47, "seek": 121292, "start": 1189.82, "end": 1212.92, "text": "و احنا ما استخدمنا اي مادة كيميائية اعتبرنا ال water هو ال decalryzer بعدها هضيف ال counterstain للصفرانين لمدة من 1 ل 2 من 1 ل 2 minute بعدها هغسل بالwater هعمل air drying على ال oil immersion يبقى الصفرانين هي counterstain", "tokens": [2407, 1975, 5016, 8315, 19446, 44713, 9778, 40448, 8315, 1975, 1829, 3714, 18513, 3660, 9122, 32640, 1829, 16373, 10632, 1975, 34268, 26890, 8315, 2423, 1281, 31439, 2423, 979, 304, 627, 4527, 39182, 11296, 8032, 11242, 33911, 2423, 5682, 372, 491, 24976, 9381, 5172, 2288, 7649, 9957, 32767, 41891, 9154, 502, 5296, 568, 9154, 502, 5296, 568, 3456, 39182, 11296, 8032, 17082, 3794, 1211, 20666, 8002, 8032, 25957, 1211, 1988, 22709, 15844, 2423, 3184, 40348, 7251, 3555, 4587, 7578, 31767, 5172, 2288, 7649, 9957, 39896, 5682, 372, 491], "avg_logprob": -0.26740056614984165, "compression_ratio": 1.6354679802955665, "no_speech_prob": 1.8477439880371094e-06, "words": 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cell البكتيريا اتدمرت بواسطة ال heat أو ال steam بالتالي سهل على الصبغة أنها تروح من ال vegetative cell بدون الحاجة لأي مادة كيميائية لأن ال malachite green هي water soluble", "tokens": [303, 847, 1166, 2815, 362, 668, 12631, 538, 3738, 570, 295, 341, 1778, 264, 2806, 608, 642, 3092, 6676, 3612, 490, 264, 5764, 1166, 2815, 4724, 39648, 32239, 14739, 2423, 5764, 1166, 2815, 29739, 4117, 2655, 13546, 25528, 1975, 2655, 40448, 43500, 4724, 14407, 3794, 9566, 3660, 2423, 3738, 34051, 2423, 11952, 20666, 2655, 6027, 1829, 8608, 3224, 1211, 15844, 31767, 3555, 17082, 3660, 14739, 11296, 6055, 32887, 5016, 9154, 2423, 5764, 1166, 2815, 47525, 11536, 21542, 26108, 3660, 5296, 10721, 1829, 3714, 18513, 3660, 9122, 32640, 1829, 16373, 10632, 5296, 33456, 2423, 2806, 608, 642, 3092, 39896, 1281, 24807, 638], "avg_logprob": -0.17371324069944083, "compression_ratio": 1.6892430278884463, "no_speech_prob": 2.384185791015625e-07, "words": [{"start": 1434.51, "end": 1435.27, "word": "vegetative", 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diff --git a/PL9fwy3NUQKwb0RqFErSm_cqoiTXH0hLcc/Lh4oT_Gdlo4_raw.json b/PL9fwy3NUQKwb0RqFErSm_cqoiTXH0hLcc/Lh4oT_Gdlo4_raw.json
new file mode 100644
index 0000000000000000000000000000000000000000..c28ab7815504a595a67e5f5c4425ed4a1592a6cb
--- /dev/null
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@@ -0,0 +1 @@
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إلى هناك يساهمون في تثبيت الوجود الفلسطيني في المدينة وانعاشها اقتصاديا وحماية المسجد الأقصى والمقدسات في مدينة القدس", "tokens": [40228, 22653, 6225, 11622, 37977, 25062, 3215, 3794, 8978, 29538, 21136, 1863, 2407, 9307, 6225, 11622, 37977, 25062, 3215, 3794, 18871, 6024, 114, 5172, 3660, 6024, 118, 25513, 10632, 18871, 3714, 5016, 1829, 9566, 11296, 18863, 2288, 21292, 6225, 11622, 37977, 20666, 4117, 10943, 1211, 20193, 7251, 14851, 9566, 40228, 14739, 7251, 36520, 11296, 11933, 15040, 8608, 41361, 25062, 3215, 3794, 5551, 3224, 1211, 25062, 3215, 3794, 8717, 36178, 16095, 36725, 3224, 1211, 8315, 8978, 16712, 2304, 7649, 10632, 4032, 9640, 3555, 3615, 9957, 8032, 2304, 32545, 9957, 37495, 22653, 7251, 36520, 11536, 30731, 34105, 4117, 7251, 3794, 995, 16095, 11536, 8978, 6055, 12984, 21292, 2655, 2423, 29245, 23328, 27188, 1211, 3794, 9566, 9957, 1829, 8978, 9673, 3215, 9957, 3660, 4032, 7649, 3615, 33599, 11296, 1975, 38149, 9381, 18513, 25528, 4032, 5016, 15042, 10632, 9673, 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