text: 3.2.
  Cell line and culture
  DU145 (prostate cancer) cell line was used in this assay. DU145 was grown in DMEM/F-12 medium (Gibco BRL Life Technologies, San Diego, CA., USA) supplemented with 10% fetal calf serum and kept in a humidified 37 degreesC, 5% CO2 incubator.
  
entities.text: ['Cell line']
entities.type: Cell
entities.entity_id: PMC-2769146-sec-05_T1
entities.text: ['DU145 (prostate cancer) cell line']
entities.type: Cell
entities.entity_id: PMC-2769146-sec-05_T2
entities.text: ['DU145']
entities.type: Cell
entities.entity_id: PMC-2769146-sec-05_T3
entities.text: ['serum']
entities.type: Organism_substance
entities.entity_id: PMC-2769146-sec-05_T4
entities.text: ['fetal']
entities.type: Developing_anatomical_structure
entities.entity_id: PMC-2769146-sec-05_T5