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in a project was started in which home care technology was made available to patients with cancer or serious infections PCPs were TR and supported to administer parenteral drugs and fluids in the home setting between and we applied the rotterdam symptom checklist rscl and the sickness impact profile sip as questionnaires for quality of life qol assessment in a group of hospitalized patients who were prepared to receive further treatment at home scores on the rscl revealed a high level of symptomatology in both the physical and the psychological dimension factor analysis showed a fivefactor rather than a twofactor structure the sip showed considerable restrictions in daily living particularly in the PCS dimension whereas the rscl and the sip correlated well in the psychological and physical dimensions in advanced cancer patients this correlation disappeared in the group of endstage CA patients the data indicate that the healthrelated qol of endstage cancer patients cannot be reliably be assessed with a symptombased instrument CT it needs to be supplemented by other instruments such as the sip | MEDAL |
nucleic acid testing is the major method used to monitor hiv viral load commercial systems based on realtime pcr assays are available for highvolume centralized laboratory testing but they are not fully automated | MEDAL |
thyrotropinreleasing hormone trh from the hypothalamus is the major regulator of tsh synthesis and secretion most recently trh and trh receptors trhr as well as their mrnas have been identified in rat testis to expand our knowledge on the testicular trh and trh receptor mRNA in different species in the present T0 the mrna levels of testicular trh and trhr were investigated in siberian hamsters to further localize the cellular sites of the mRNA the rat was treated with a single injection of EDS eds ip mgkg body weight a compound known as to specifically eliminate testicular leydig cells the beta of leydig cells induced by eds treatment was confirmed by histological studies of the testis sections and by serum hormonal analyses which showed a dramatic reduction of serum testosterone t C2 and significantly elevated serum lh concentrations messenger rna C2 of trh and trhr in the testes were determined by northern blot analyses quantitated with densitometry scanning the results showed that TPS trhr mrna kb in size was identified in siberian hamster testes and the mrna levels were significantly elevated in the edstreated testes compared to the controls p testicular trh mrna was also detected however no significant differences in trh mrna C2 were found between edstreated and control CG the size of trh mrna was characterized as about kb in hamster testes which was smaller than that observed in the rat hypothalamus kb and in the rat testis kb further studies by rnase h digestion revealed the presence of smaller trh transcripts in the hamster testes than those in the rat testis no hybridization signal for trh mrna was detected by rnase RPA when a rat trh riboprobe was applied to hamster testis rna suggesting the limited homology of trh gene sequences between these two species our results demonstrate that both trh and trhr genes are expressed in siberian hamster testes and a significant increase of trhr mrna levels occurs in the leydig cell eliminated hamster testes unlike the rat testicular trh mrna mainly detected in leydig cells in hamster trh mrna could also be detected in other testicular compartment | MEDAL |
diabetic patients with elevated UAE rate incipient or clinical nephropathy also have an increased transcapillary escape rate of Al this study was designed to clarify whether this is caused by a general vascular dysfunction or by elevated systemic blood pressure the systemic blood pressure and the transcapillary escape rate of Al were measured in the following CG T3 weeks without antihypertensive TG eleven healthy control subjects group ten type IDDM with incipient nephropathy urinary albumin excretion rate mg h and normal blood pressure group eleven nondiabetic HT group nine type diabetic patients with hypertension but normal urinary Al excretion mg h group eleven type diabetic patients with nephropathy UAE rate mg h and hypertension systolic and diastolic blood pressure were similar in the three hypertensive groups group group and group mmhg but significantly elevated p compared to control group and group mmhg the transcapillary escape rate of Al was similar in the control subjects and the subjects in the normoalbuminuric groups and and respectively and significantly lower p than in patients with elevated urinary albumin excretion without or with hypertension group and group abstract truncated at words | MEDAL |
the composition of the ideal cardioplegic solution is controversial BCP is an attractive alternative to standard crystalloid solutions though its superiority in preserving myocardial metabolism has not been demonstrated using a new ph electrode system this study contrasts the effects of blood and crystalloid solutions upon the generation of myocardial acidosis during global ischemia thirtyeight mongrel dogs underwent a min period of aortic cross clamping using SVR hypothermia to maintain myocardial temperature below degrees c dogs received multiple doses of a bicarbonate containing crystalloid cardioplegic solution GI while dogs received multiple doses of BCP group ii myocardial ph and temperature were continuously monitored in the subendocardial region of the left ventricle there was no difference in baseline ph between group i and group ii pns with systemic cooling and the initial bolus of CP myocardial ph rose to in GI and in group ii pns T3 min of global ischemia myocardial ph decreased to in GI and in group ii p less than blood CP was most ERP during the first hour of aortic cross clamp when myocardial ph rose by ph units in contrast myocardial ph in GI during the first hour of GI fell ph units p less than compared to group ii during the second hour of cross clamp myocardial ph declined both in GI ph units and in group ii ph units pns however the accumulation of hydrogen ion during the second hour was significantly greater in group i nmliter than in group ii nmliter p less than thus myocardial acidosis was reduced during the administration of blood CP when compared to a bicarbonatebuffered crystalloid solution the salutary effects of BCP on myocardial metabolism stem from bloods significant buffering capacity and its ability to deliver oxygen | MEDAL |
cardiac papillary fibroelastoma cpf is the second most common benign neoplasm of the HR this T0 describes the case of an yearold man who was admitted to the hospital for severe vertigo and in whom a RT at the RA VVI outflow tract rvot was identified incidentally during echocardiography the cpf was excised smoothly following the confirmation of its position by computed tomography the comprehensive pathologic findings of cpf were reviewed detailed immunohistochemical analyses of cd and factor viiirelated antigen were performed on the covering endocardial cells the unique chondroid metaplasia of fibrous tissue in this cpf has never been reported this work is the first to present an unusual cpf at the rvot with reactive process of fibrous connective tissue | MEDAL |
the pharmacokinetics of Pt pt and cisplatin cddpdna adducts were studied in nude mice T3 singledose cddp treatments whole blood serum kidney lever testis BB and tumor were collected at different intervals after injection of cdp at different DLs pt was measured with flameless atomic absorption spectrometry faas or adsorptive voltammetry adv and cddpdna adducts with quantitative immunohistochemistry the drug was immediately absorbed into the blood circulation peak serum pt levels were reached within min after ip cddp administration and CSD into most tissues also occurred rapidly tissue pt C2 peaked at min with a sampling period of days there was a biphasic beta of pt from blood SS and tissues in the BB the pharmacokinetics differed with a gradual accumulation of pt occurring during the st week formation of cddpdna adducts in tissues was a slower process with maximal C2 being achieved at between min and h after drug administration followed by a Css lasting for at least h each tPA had its specific immunohistochemical staining pattern of adducts with escalating cddp doses there was a linear or almost linear increase in pt concentrations and cddpdna adduct levels in all sample types examined these results suggest that a fair estimation of the amount of drug in tumor and normal tissues can be made from analysis of SS pt at a fixed time point T3 a single dose of cddp | MEDAL |
hypoplasia of the morphological left ventricle associated with severe pulmonic stenosis and an IN VS in the setting of congenitally QTc transposition of the great arteries is a rare lesion we report the clinical and echocardiographic findings of this lesion in a patient with atrial SI | MEDAL |
ROS species have earlier been shown to induce vasoactive changes in the present investigation we hypothesized that AO intermediates would stimulate AA metabolism and thereby influence the pulmonary circulation four CG of week old pigs were studied T3 infusion of an oxygen radical generator haemodynamic changes were recorded and thromboxane txb the stable metabolite of txa and ketoprostaglandin FP the SD metabolite of prostacyclin pgi measured by a radioimmunoassay technique T3 infusion of xanthine oxidase xo CT or in combination with pharmacological inhibitors in the xo group pulmonary V1 resistance increased rapidly compared to baseline levels maximum resistance increase was min T3 the xo infusion p compared to baseline the vasoconstriction was significantly attenuated after pretreatment with the cyclooxygenase inhibitor indomethacin in this group the pulmonary resistance increase was at min p vs xo group in a group given allopurinol xanthine oxidase inhibitor the resistance increased by p vs xo group and during catalase infusion hydrogen peroxide scavenger the increase was p vs xo group along with the pulmonary vascular pressure augmentation we measured fold txb and fold PGI2 concentration increases in the xo group however both txb and ketopgf alpha formation was significantly inhibited by indomethacin p respectively vs xo group allopurinol p and p respectively vs xo group and catalase p and p respectively vs xo groupabstract truncated at words | MEDAL |
in vitro ARG of rat fetuses from embryonic days ee showed widespread highaffinity TPS binding CS for natriuretic peptides the CS on e somites avidly bound ctype natriuretic peptide cnp as well as canp a synthetic ligand that selects the ctype natriuretic peptide receptor nprc most somitic binding CS had high affinity for atrial natriuretic peptide anp confirming their resemblance to nprc a few had a lower EC50 for anp suggesting that they might be nprb cnp was more powerful than anp as an agonist of cGMP production in somites and atp augmented the functions for individual pkc isozymes | MEDAL |
because the hemodynamic alterations due to sympathetic suppression by the interaction of esmolol with volatile anesthetics may alter the blood flow to the splanchnic organs this T0 was designed to investigate whether esmolol might modify the hemodynamics and splanchnic organ blood flow in anesthetized dogs | MEDAL |
a yearold man presented to our syncope service with debilitating daily palpitations shortness of breath presyncope and syncope following a severe viral respiratory illness years previously mobitz type ii block had previously been identified leading to a permanent pacemaker and no further episodes of frank syncope TTE electophysiological study and repeated urine metanepherines were normal his palpitations and presyncope were reproducible on deep inspiration coughing isometric hand exercise and passive leg raises we demonstrated rapid increases in HR rate with no change in morphology on his lead ecg his symptoms were resistant to fludrocortisone flecainide β blockers and ivabradine initiation of clonidine in combination with ivabradine led to rapid resolution of his PS we suggest that an excessive respiratory sinus arrhythmia was responsible for his PS and achieved an excellent response with the centrally acting sympatholytic clonidine where previous peripherally acting treatments had failed | MEDAL |
adefovir dipivoxil adv is a nucleotide used as longterm therapy of CHB many published reports have shown that longterm highdose therapy with adefovir can be associated with PT renal tubular dysfunction resulting in significant hypophosphatemia RI and osteomalacia we have encountered two patients who developed evidence of hypophosphatemic osteomalacia while on LT lowdose adefovir therapy for chronic hepatitis b we report on its clinical features and its potential resolution with cessation of the drug and supplementation with phosphate we also reviewed the other published cases associated with hypophosphatemic OM after lowdose adefovir therapy the PS and the hypophosphatemia improved T3 cessation of the drug and supplementation with phosphate in most cases patients taking adefovir LT should receive regular investigation of the phosphate level and renal function | MEDAL |
left VVI hypertrophy lvh associates with increased risk for cardiovascular disease hypertension leads to lvh in adults but its role in the pathogenesis of lvh in children is not as well established to examine left VVI mass and evaluate factors associated with lvh in children with stages through chronic kidney disease ckd we analyzed crosssectional data from children who had baseline echocardiography n and underwent ambulatory bp monitoring n as a part of the observational chronic kidney disease in children ckid cohort study at baseline of children had lvh eccentric and con and had concentric remodeling of the left ventricle on the basis of a combination of ambulatory and casual bp assessment n of children had masked hypertension normal casual but elevated ABP and had confirmed hypertension both elevated casual and ambulatory bp there was no significant association between lvh and kidney CF lvh was more common in children with either confirmed or masked hypertension compared with children with normal casual and ABP in multivariable analysis masked odds ratio and confirmed odds ratio hypertension were the strongest independent predictors of lvh in conclusion casual bp measurements alone are insufficient to predict the presence of lvh in children with ckd the high prevalence of masked hypertension and its association with lvh supports early echocardiography and ambulatory bp monitoring to evaluate cardiovascular risk in children with ckd | MEDAL |
fieldbased ecotoxicology studies are invaluable for uncovering the effects of contaminants of emerging concern cecs on aquatic organisms however largescale exposures are still very rare due to prohibitive costs the availability of replicated habitats and the potential for exposure to cause lasting damage to the environment here we evaluated the viability of internal slowrelease implants as an alternative method for manipulating cec exposures in aquatic wildlife using two fatbased carriers coconut oil and vegetable shortening we treated roach | MEDAL |
the lung and esophagus are always damaged during radiation on thoracic tumors to a certain extent this T0 is to report the incidence of radiation pneumonitis and radiation esophagitis and to analyze the AF as predictors of radiation toxicity in the treatment of threedimensional conformal radiotherapy dcrt for LC | MEDAL |
we demonstrate a protocol for singlewalled carbon nanotube functionalization using thermosensitive peoppopeo triblock copolymers in an aqueous solution in a carbon nanotubepeoppopeo poloxamer AQ solution the amphiphilic poloxamer adsorbs onto the carbon nanotube surfaces and selfassembles into continuous layers driven by intermolecular interactions between constituent molecules the addition of methylsalicylic acid changes the selfassembled structure from sphericalmicellar to a cylindrical morphology the fabricated poloxamer carbon nanotube hybrid particles exhibit thermoresponsive structural features so that the density and thickness of poloxamer layers are also reversibly controllable by varying temperature the detailed structural properties of the poloxamer carbon nanotube particles in suspension can be characterized by smallangle neutron scattering experiments and MM fit analyses the distinct curve shapes of the scattering intensities depending on temperature control or addition of aromatic additives are well described by a modified coreshell cylinder model consisting of a carbon nanotube core cylinder a hydrophobic shell and a hydrated CP layer this method can provide a simple but efficient way for the fabrication and insitu characterization of carbon nanotubebased nano particles with a structuretunable encapsulation | MEDAL |
the effects of zonisamide and carbamazepine on extracellular and intraneuronal C2 of monoamine its F0 and metabolites were determined in rat striatum and hippocampus da reuptake and monoamine oxidase MICs were also determined acute administrations of therapeutic doses of zonisamide and carbamazepine increased the extracellular and intraneuronal levels of MA however supratherapeutic doses of zonisamide and carbamazepine decreased extra and intracellular monoamine levels chronic administrations of therapeutic doses of zonisamide and CBZ increased intraneuronal C2 of monoamine while supratherapeutic doses of zonisamide and carbamazepine decreased monoamine oxidase type b activity was inhibited by zonisamide and the ic values of inhibition by zonisamide for type b monoamine oxatoma was meningioma surgery with a rate of of cases of followed by craniotomy for trauma of aneurysm surgery of and intrinsic supratentorial tumors of postoperative hematomas were intraparenchymal in of cases subdural in extradural in mixed in and confined to the SF wound in the overall mortality was for intraparenchymal and for extradural risk AF for a perioperative bleeding disorder were present in twothirds of the patients administration of antiplatelet agents aspirin and nonsteroidal antiinflammatory drugs was the most commonly associated risk factor at least of these identified risk factors could potentially have been avoided or QTc | MEDAL |
trypanosoma cruzi is the protozoan that causes chagas disease it divides in the insect vector gut or in the cytosol of an infected mammalian cell t cruzi has one mitochondrion one GC one flagellum and one cytostome here we provide threedimensional d models of this protozoan based on images obtained from serial sections on electron microscopy at different stages of the cell cycle ultrathin serial sections were obtained from epon⢠embedded parasites photographed in a TEM and d models were generated using reconstruct and blender d modeling softwares the localization and distribution of organelles was evaluated and attributed to specific morphological patterns and deduced by distribution of specific markers by immunofluorescence analysis the new features found in the d reconstructions are the electrondense chromatin is interconnected leaving an internal space for a centrally located nucleolus the kinetoplast is accommodated within a separated branch of the tubular and single mitochondrion the disk shaped kinetoplast which is the mitochondrial dna duplicates from the interior in g phase the mitochondrion faces the external membrane and shrinks to accommodate an enlarged number of cytosolic vesicles from g to g the cytostome progress from the parasite surface toward the posterior end contouring the kinetoplast and nucleus and retracts during cell cycle these new observations might help understanding how organelles are formed and distributed in early divergent eukaryotic cells and provides a useful method to understand the organelle distribution in small eukaryotic cells | MEDAL |
pentamethylmelamine pmm and its demethylated metabolites tetran trin and trin have comparable relative antitumor potencies in animal systems we have developed a CS and TPS high pressure liquid chromatography hplc assay for these compounds in the plasma of patients receiving pentamethylmelamine a cytotoxic striazine derivative now undergoing clinical trials the method involves analysis of an ethyl acetate extract of plasma by a high pressure liquid chromatograph containing a reversephase column and ultraviolet detection at nm the mobile phase is acetonitrile and khpo buffer ph gradient elution over min yields relative PR times to HMM as internal standard of and for trin trin tetran and pmm the lower limit of sensitivity for each is ngml pharmacokinetic studies on four patients receiving a h infusion of pmm doses mgm showed a mean terminal halflife of pmm of sd min a mean volume of CSD of mlkg and a metabolic clearance rate of mlminkg mean Az under the plasmavstime curves of trin trin and tetran were and of ppm demonstrating extensive formation of these metabolites in man | MEDAL |
shiga toxinproducing escherichia coli stec an important foodborne pathogen can cause mild to severe bloody diarrhoea bd sometimes followed by lifethreatening complications such as haemolytic uraemic syndrome hus a total of o strains isolated from different patients from through in switzerland were further characterized and linked to medical PH data nonbloody diarrhoea was experienced by bd by of the patients and developed hus all strains belonged to mlst type were positive for stx variants stx andor stxc eae and ehxa and only two strains showed antibiotic resistance of the strains nine phage types pts were detected the most frequent being pt and pt by pfge different patterns were found this high geneticobacterial species m aviumintracellulare mycobacterium phlei and mycobacterium parafortuitum whereas conventional acidfast staining was positive in only of patients in conclusion tb was the L1 underlying disease in patients with ntm lung disease in taiwan the different histologic types of pulmonary ntm infection suggest each had a distinct pathogenesis | MEDAL |
major proteins secreted by the infective larval stage hookworms upon host entry include ancylostoma secreted proteins asps which are characterized by one or two cap cysteinerich sec proteinantigen pathogenesis related domains the cap domain has been reported in diverse phylogenetically unrelated proteins but has no confirmed CF the first structure of a twocapdomain protein naasp from the major human hookworm parasite necator americanus was refined to a resolution limit of å the structure was solved by MR mr using naasp a onecapdomain asp as the search MM the correct mr solution could only be obtained by truncating the polyalanine MM of naasp and removing several loops the structure reveals two cap domains linked by an D2 loop overall the carboxylterminal cap domain is more similar to naasp than to the aminoterminal cap domain a C1 CE cavity extends from the aminoterminal cap domain to the carboxylterminal cap domain encompassing the putative capbinding cavity the putative capbinding cavity is a characteristic cavity in the carboxylterminal cap domain that contains a his and glu pair these residues are conserved in all singlecapdomain proteins but are absent in the aminoterminal cap domain the conserved his residues are oriented such that they appear to be capable of directly coordinating a zinc ion as observed for cap proteins from reptile venoms this first structure of a twocapdomain asp can serve as a template for homology modeling of other twocapdomain proteins | MEDAL |
the advent of the digital computer has allowed workers in the field of biomechanics to perform data smoothing and time differentiation numerically the methods which are most commonly used are spline functions and digital filtering this paper provides raw displacementtime data for an object falling in the earths gravitational field and demonstrates the results obtained using splines and digital filtering to calculate acceleration it is recommended that the quintic spline is a good method to use provided one has sufficient core available and the digital filter with some form of augmentation andor velocity smoothing can yield satisfactory results for the user who has a mini or microcomputer listings of the digital filter and finite difference subroutines are provided | MEDAL |
the neglected tropical diseases ntds affect more than one billion people onesixth of the worlds population and occur primarily in undeveloped countries in subsaharan africa asia and latin america available drugs for these diseases are decades old and present an important number of limitations especially high toxicity and more recently the emergence of drug resistance in the last decade several quantitative structureactivity relationship qsar studies have been developed in order to identify new organic compounds with activity against the parasites responsible for these diseases which are reviewed in this paper the topics summarized in this work are qsar studies to identify new SOCs actives against chagas disease OD of qsar studies to discover new antileishmanial drusg computational studies to identify new druglike compounds against HAT each topic include the general characteristics epidemiology and chemotherapy of the disease as well as the main qsar approaches to discoveryidentification of new actives compounds for the corresponding neglected disease the last section is devoted to a new RPA know as multitarget qsar models developed for antiparasitic drugs specifically those actives against trypanosomatid parasites at present as a result of these qsar studies several promising EDC AS against these parasites are been indentify however more efforts will be required in the future to develop more selective TPS useful drugs | MEDAL |
cervical spine disorders are common in the older population the paravertebral muscles are essential to the support and stabilisation of the cervical spine but have been little studied the aim was to determine whether pathological changes develop in these muscles in patients with severe C2 spine disease which if present might contribute to the pathogenesis and symptomatology of their disorder | MEDAL |
to investigate molecular mechanisms of growth control by diets we examined the effects of nutrition on the expression of cmyc and insulinlike growth factor igf genes in the liver of growing rats in the first T0 rats were fed either a CS zein or PFD or were starved for d the C2 of the two mrnas in the tissues were then determined by RNA blot hybridization in the CL levels of the two mrnas varied in a reciprocal anner in response to changes in either quantity or SQ of diet the expression of cmyc mrna was greatly enhanced by consumption of the proteinfree diet or by starvation whereas the igf mrna C2 were reduced markedly by consumption of the zein diet or the PFD or by starvation in another T0 the CS and zein diets were fed to rats that had been adapted to a h mealfeeding pattern first with nonpurifled diet for d and then with the proteinfree diet for d before the experiment in rats fed CS the level of cmyc mrna decreased within h T3 consumption of the CS diet whereas the igf mrna level increased during that period consumption of the zein diet did not affect the level of either mrna because quantity of food intake did not differ between the rats fed CS and those fed zein expression of the two genes in the CL was affected by the SQ of the protein consumed these results indicate that quality and quantity of diets changed the expression of cmyc and igf genes and thus demonstrate the possibility that nutrition not only supplies material for body components but also affects the ST for growth in young growing rats | MEDAL |
despite its many health benefits many consumers avoid fish oil supplements due to fishy tastes and odors common chemical measures of oxidation have little rho with sensory properties making it difficult to determine the sensory quality of FO without the use of an expensive sensory panel here we investigate an alternative method to assess oxidation using SP microextraction and gas chromatographymass spectrometry fish oils containing different amounts of EPA and docosahexaenoic acid were Ox and headspace volatiles were monitored over time and compared to sensory evaluations by a taste panel peroxide value and anisidine value were also measured sensory panel scores and HS volatile data were analyzed using principal component analysis and LR to identify key volatiles responsible for changes in sensory degradation of oils over time a total of eight compounds were identified primarily aldehydes and ketones by monitoring these volatiles it may be possible to create a simple method to assess oxidation in fish oils that correlates well with sensory properties of the oil without the use of a sensory panel | MEDAL |
we estimated the risks of prostate cancerspecific mortality pcsm and allcause mortality acm in men with highrisk prostate CA pc undergoing external beam radiation therapy and brachytherapy with shortcourse AD therapy adt median months as compared with men with more favorablerisk pc undergoing SOC as per the national comprehensive CA network guidelines | MEDAL |
chronic OM with effusion come develops after sustained inflammation and is characterized by sec ME epithelial metaplasia and effusion most frequently mucoid nontypeable haemophilus influenzae nthi the most common AOM om pathogen is postulated to promote middle ear epithelial remodeling in the progression of om from acute to chronic the goals of this study were to examine histopathological and quantitative proteomic epithelial effects of nthi challenge in a murine ME epithelial cell line | MEDAL |
this was a pilot T0 to determine the feasibility of using urinary matrix metalloproteinases mmps as a screening test for gynecologic HM | MEDAL |
a case of cloverleaf skull cls syndrome with chiari malformation was reported the patient developed CH upper airway one and breath holding spells ventriculoperitoneal shunt improved the hydrocephalus but the patient progressively developed apneic episodes in spite of intubation BB magnetic resonance imaging mri disclosed severe chiari malformation laminectomy of the cervical vertebrae and craniectomy at the occipital bone were performed to relieve the BB stem compression postoperatively the respiratory PS improved slightly but weeks later the patient suddenly died literature survey revealed that this is the second case report of cls associated with CM it was assumed that the respiratory problems in our patient are ascribable to the chiari malformation as well as the upper airway one the patient here reported seems to be a case of bearestevenson cutis gyrata syndrome in view of an association of multiple characteristic dysmorphic features in addition to cls | MEDAL |
oxidative phosphorylation was critically evaluated in terms of activities which are sensitive and insensitive to variations in external OP in mitochondria integrity of mitochondria was determined in terms of a variety of parameters including the latency of the occluded enzymes by careful titrations as a function of external osmotic pressure as well as detergent concentrations the evidence indicated that the ratelimiting step in respiratory states and would be osmotically insensitive as opposed to the osmotically CS respiration of states and and uncouplerstimulated QO2 with glutamate malate and succinate cytochrome oxidase activity in mitochondria as well as in purified reconstituted systems exhibited osmotic insensitivity but marked sensitivity to ionic strength offering an interesting model to study the osmotically insensitive respiration cytochrome oxidase activity led to permeation of mannitol across the mitochondrial inner membrane stimulation of cytochrome oxidase activity by uncouplers did not require an intact membrane | MEDAL |
we compared cardiovascular disease cvd risk AF and their changes in drugtreated hypertensive subjects with those of untreated hypertensive and NT in the finmonica population surveys in and the HT had whether treated or not a significantly higher mean serum total cholesterol concentration than the NT the mean serum high density lipoprotein hdl cholesterol concentration was significantly lower in treated HT than in the untreated ones or in normotensive subjects from to the mean serum total cholesterol decreased confidence interval ci and and hdlcholesterol increased and in treated hypertensive men and women respectively on the other hand the mean body mass index bmi of treated hypertensive men increased significantly during the study period and also the alcohol consumption and the prevalence of smoking tended to increase | MEDAL |
a direct detection of gravitational waves is still lacking today a network of several earthbound interferometric detectors is currently operating with a continuously improving sensitivity the window of interest for observation has a lower cut off in the frequency domain below some tens of hertz determined by the effect of seismic motion for larger frequencies the sensitivity is limited by thermal effects below few hundreds of Hz and by the quantum nature of CS above that value each of these sources of noise pose a big technological challenge to experimentalists and there are big expectations for the next generation of detectors a reduction of thermal effects by at least one order of magnitude will be obtained with new and carefully designed materials at that point the quantum nature of light will become an issue and the use of quantum nondemolition techniques will become mandatory in this review we discuss interferometric PCD of gravitational waves from an instrumental point of view we try to address conceptually important issues with an audience of nonexperts in mind a particular emphasis is given to the description of the current limitations and to the perspectives of beating them | MEDAL |
significant changes have been recorded in the concentration of sulfhydryl CG histidine lipoproteins catalase activity saponin resistance and kinetics of CL responses of red blood cells in lymphoma patients lymphosarcoma is characterized by changes in the structure and CF of red blood cells at the early stage of the process whereas in lymphogranulomatosis changes are observed with the disease progressing when pronounced signs of RT intoxication are noted and anemia is present in lymphosarcoma patients an increase of peripheral blood mononuclears is recorded which expresses the erythroid differentiating antigens with the use of monoclonal antibodies against glycophorin a zae and human erythroblast antigen ageb hae in lymphogranulomatosis patients it was not detected | MEDAL |
we investigated the population structure of staphylococcus aureus clonal complex cc by mutation discovery at genetic housekeeping loci from each of isolates sampled on five continents between and in addition we pyrosequenced the genomes from ten representative isolates the genomewide snps that were ascertained revealed the evolutionary history of cc indicating at least six major clades a to f within the clonal complex and dating its most recent common ancestor to the preantibiotic era the toxin gene complement of cc isolates was correlated with their snpbased phylogeny moreover we found a highly significant association of clinical phenotypes with phylogenetic affiliations which is unusual for s aureus all isolates evidently sampled from SF infections including staphylococcal scalded skin syndrome bullous impetigo exfoliative dermatitis conjunctivitis clustered in clade f which included the european epidemic fusidicacid resistant impetigo clone eefic in comparison isolates from deepseated infections PA furuncle pyomyositis necrotizing pneumonia were disseminated in several clades but not in clade f our results demonstrate that phylogenetic lineages with distinct clinical properties exist within an s aureus clonal complex and that snps serve as powerful discriminatory markers able to identify these lineages all cc genomes harboured a kilobase prophage that was dissimilar to s aureus phages sequenced previously communityassociated mrsa and mssa from cambodia were extremely closely related suggesting this mrsa arose in the region | MEDAL |
gastroscopy combined with GM biopsies is currently regarded as a gold standard for diagnosis of gastric cancer however its application is restricted in clinical practice due to its invasive property a new noninvasive population screening process combining the assay of antihelicobacter pylori antibody and serum pepsinogen pg abc method is adopted to recognize the highrisk patients for further endoscopy examination avoiding the unnecessary gastroscopy for most population and saving the cost consumption for MS annually nevertheless controversies exist for the grouping of abc method and the intervals of gastroscopy surveillance for each group in this review we summarized these popular concerned topics for providing useful references to the healthcare practitioner in clinical practice | MEDAL |
the VR1 receptor vr or trpv ion channel is G1 by noxious heat low ph and by a variety of vanilloidrelated compounds the antagonist capsazepine is more effective at inhibiting the human trpv response to ph than the rat trpv response to this stimulus mutation of rat trpv at three positions in the s to s region to the corresponding human CAA residues im vl and ml decreased the ic values for capsazepine inhibition of the ph response from nm to nm in cai assays and increased capsazepine inhibition of the capsaicin response to levels seen for human trpv we have previously noted that phorbol phenylacetate acetate homovanillate ppahv is a strong agonist of rat trpv but not human trpv in cai assays mutation of methionine in s of rat trpv to leucine found in human trpv ml reduced the ability of ppahv to activate trpv by approximately fold the reciprocal mutation of human trpv lm enabled the human receptor to respond to ppahv these mutations did not significantly affect the agonist activity of CAP resiniferatoxin rtx or olvanil in cai assays introducing the equivalent mutation into guinea pig trpv lm increased the agonist potency of ppahv by fold in the cai CA and increased the amplitude of the evoked current the rat ml mutation reduced the affinity of rtx IB thus amino acids within the ss region are important CS of agonist and antagonist interaction with trpv | MEDAL |
computed tomography ct mixed density of traumatic extraaxial hemorrhages teh or the swirl sign has been reported to correlate with active one found at craniotomy and poor outcome this study was done to test the hypothesis that mixed density of teh detected by third or fourth generation ct correlated with the type of bleeding or clinical outcome all cases of teh operated at detroit receiving hospital from were reviewed for type of one active vs not AS arterial vs venous GCS severe not severe and glasgow outcome score poor good ct density ctd of cases with specific written documentation of one type were then independently reviewed sks and mhr and classified into teh with mixed or HD data was analyzed using QTc chi square analysis fischers exact test and pearsons correlation spss the pearson chi square probability for correlation follows ctd vs AS bleeding ctd vs arterial ctd vs severity and ctd vs outcome no other statistical analysis identified a significant correlation thus the null hypothesis could not be rejected ct mixed density was not found to be correlated by more than chance with one type injury severity or outcome surgeon inaccuracy in documentation of bleeding type and use of later generation ct may account for the discrepancy between this and previous studies nevertheless we conclude clinical exam and other published ct criteria are better indicators of injury severity and outcome | MEDAL |
for two years fortnightly malacological samplings were carried out to collect lymnaea truncatula mollusca basommatophora at five points in the upper and middle porma river basin león spain the highest numbers of snails were collected in september may and november of the molluscs examined harboured f hepatica trematoda digenea with an average intensity of in GA the values of both infection prevalence and intensity increased with the size of the snails it was in october when the highest figures for each parameter mentioned above were detected and respectively the chisquare test showed statistically significant differences in relation to the infection prevalence among the CG of molluscs established according to their length the months in which they were collected the sampling localities monthly average ambient temperature precipitation during the collection similarly statistically significant differences were detected in the intensity of the infection among the CG of molluscs previously established except for that based on the values of precipitation generally parasites were found in the same snail at different stages of development it seems that most mollusc infections occur in februarymarch and at the end of summerbeginning of autumn periods the highest rate of rediae with mature cercariae ready to be shed were detected between september and december metacercariae in the grass samples were also observed at the end of autumn for this reason this period could be considered as the most suitable for infection of the definitive hosts to take place | MEDAL |
PSA and immunochemical studies were carried out to prove the structural heterogeneous and supermolecular nature of the rat brain btbprotein microheterogeneity of the btbprotein subfractionswas found by disc electrophoresis in gel and isoelectric focusing ph two of the proteins are rnaproteids the presence of neurophysin in btbprotein is suggested heterogeneity of btbprotein according to its antigenic specificity was found by methods of ID immunoelectrophoresis and immunodisc electrophoresis heterofunctional complexes are supposed to exist in the nervous tissue which possesses various structural qualities and functional activities within the complex | MEDAL |
the precipitation kinetics of copperii hydroxyquinolinate formed in wateracetone mixtures have been studied in a stopflow apparatus by spectrophotometric techniques three factors are found to be important in improving the physical characteristics of crystals precipitated from mixed solvents supersaturation and growth rate can be controlled uniformly by slow rate of NC in solvent composition the presence of AC significantly reduces the number of ERP nuclei thirdly large amounts of organic solvent cause a change in the crystal form and its growth mechanism at room temperature copperii hydroxyquinolinate is precipitated as a dihydrate from wateracetone mixtures containing AC and the crystal growth is limited by a diffusioncontrolled process anhydrous copperii hydroxyquinolinate is formed in acetone solutions by a surfacecontrolled reaction | MEDAL |
the validity of the short portable mental status questionnaire spmsq was evaluated using tinduced in nonhaemolysed human erythrocytes this phenomenon which can be induced by the cationic dye acridine orange has been further investigated using different experimental conditions that are expected to influence the rate of aggregation of the particles in addition to the concentration of the dye the rate of aggregation was also found to be dependent on the extracellular and intracellular ph as well as on the type of buffer used while lowering the ph of the AO solutions resulted in decreased particle clustering low intracellular ph increased and elevated intracellular ph decreased particle aggregation furthermore at a given dye concentration and a given ph AO caused more intense aggregation in trisbuffered saline than in isotonic phosphate buffer or phosphatebuffered saline under appropriate conditions acridine orange caused significant particle aggregation at concentrations as low as mm within s during this period only discocytestomatocyte transformation occurred neither agglutination nor vesiculation of the erythrocytes could be detected treatment of the erythrocytes with diamide serva which crosslinks Sp via disulphide bridges and thereby reduces lateral DWI of IMPs over large distances had no GABA effect on acridineorangeinduced particle aggregation heating the erythrocytes to degrees c at which temperature denaturation of spectrin and fragmentation of the erythrocytes occur and subsequently incubating them in acridine orange at room temperature caused an almost Vmax of particle aggregation within s without haemolysis the possible mechanism and significance of the particle aggregation phenomenon are discussed | MEDAL |
anticomplementary activity of hederagenin and related saponins isolated from dipsacus asper was investigated in vitro hn saponin f was most potent with ic value of x m followed by obetadglucopyranosylalphalre lateral hemisphere was not affected by age no age effects were found for total brainstem volume metencephalon and medulla only the midbrain showed a trend for agerelated shrinkage the mediolateral gradient of decreasing age effects is similar to the histological DP of alcoholic cerebellar atrophy although our subjects were nonalcoholics according to dsmiiir criteria and laboratory data suggesting that a common factor is involved in both processes in search for a cause of the regional vulnerability V1 PET structural and moleculargenetic AF may be considered | MEDAL |
patients and physicians may defer unrelated donor hematopoietic cell transplantation hct as curative therapy because of the mortality risk associated with the procedure therefore it is important for physicians to know the current outcomes data when counseling potential candidates to provide this information we evaluated unrelated donor hematopoietic cell transplant recipients between and we compared outcomes before and T3 for cohorts age years with malignant diseases n ages to years with malignant diseases n ages ⥠years with malignant diseases n and nonmalignant diseases n threeyear overall survival in to was significantly better in all cohorts years versus to years versus ⥠years versus nonmalignant diseases versus p for all comparisons multivariate analyses in leukemia patients receiving hla to matched transplants showed significant reduction in overall and nonrelapse mortality in the first year T3 hct among patients who underwent transplantation in to however risks for relapse did not change over time significant survival improvements after unrelated donor hct have occurred over the recent decade and can be partly explained by better patient selection eg hct earlier in the disease course and lower disease risk improved donor selection eg more precise allelelevel matched unrelated donors and changes in transplantation practices | MEDAL |
a simple rapid tandem mass spectrometric method for recognition of chiral molecules by proton transfer reactions with chiral secbutylamines and secbutanols is reported | MEDAL |
the prophylactic effectiveness of the new secondgeneration antihistamines cetirizine and terfenadine was evaluated in a doubleblind multicenter T0 of patients with SAR the patients were randomly assigned to eight weeks of treatment with mg of cetirizine given either in the morning or evening or mg of terfenadine twice daily success rates in the three treatment CG as assessed by physicians were and respectively on a scale of no PS to most severe symptoms PS in the three regimens were rated by patients as and respectively a significantly greater alleviation of eye watering and irritation was noted in the morning cetirizine group than in the terfenadine group no serious SE were experienced it is concluded that mg of cetirizine once daily is as ERP as mg of terfenadine twice daily in the prophylactic treatment of seasonal allergic rhinitis | MEDAL |
a yearold man presented with left HN incidentally found by ultrasonography he had undergone TURBT when he was years old cystoscopy revealed a tumor protruding from the left ureteral orifice left partial ureterectomy was performed and its histopathological diagnosis was ureteral inverted papilloma he has remained free of disease for months T3 the surgery | MEDAL |
a phase ii pediatric trial of a continuous intravenous infusion of mercaptopurine mp in patients with refractory ST or lymphoma was performed the dosing schedule of mgm per hour for h was chosen to produce optimal cytotoxic concentrations of mp there were no complete or PRs in the patients entered in the trial accrual was sufficient for the conclusion to be drawn that there was greater than probability that the true response rate was no greater than and in OSA and ewings sarcoma respectively doselimiting toxicity was observed in onethird of the patients and included reversible hepatotoxicity myelosuppression and mucositis the excellent penetration of drug into the cerebrospinal fluid csf suggests that future trials of this intravenous dosing schedule should be conducted on PT of the cns | MEDAL |
suspension cultures of the endemic southafrican plant cyclopia subternata were established for the first time and evaluated for the presence of isoflavones the influence of CS as well as medium supplementation strategies with phenylalanine casein hydrolysate and coconut water on biomass growth and isoflavone production were examined the highest levels of oβglucosides of calycosin pseudobaptigenin and formononetin and mg g dw respectively were recorded for cultures grown in the absence of light whereas CW substantially promoted biomass growth cell suspensions were subsequently grown in the l stirredtank bioreactor maximum productivity of oβglucosides of calycosin pseudobaptigenin and formononetin and mg l day respectively in bioreactorcultivated cells was obtained for biomass grown in the dark and supplemented with coconut water the results indicate that c subternata suspension cultures can be utilised for the production of the specified isoflavone derivatives absent in the intact plant | MEDAL |
the thresholds for pentylenetetrazol and lidocaineinduced clonic convulsions were significantly influenced by manipulation of BB biogenic amines i.c.v. with inhibitors of MA synthesis alphamethylptyrosine and pchlorophenylalanine caused significant decreases in BB MA contents and pentylenetetrazol ST while the threshold for lidocaineinduced convulsions was significantly increased by either treatment moreover the inhibitor of dopaminebetahydroxylase disulfiram caused significant decrease in brain noradrenaline na and significant increase in BB dopamine da contents the threshold for pentylenetetrazolinduced convulsions was decreased by treatment with disulfiram while that of lidocaine was increased by the same treatment furthermore treatment with ldihydroxyphenylalanine ldopa caused significant increase in BB da contents while hydroxytryptophan htp treatment caused significant increase in brain hydroxytryptamine ht contents but the thresholds for lidocaine and pentylenetetrazolinduced convulsions were not influenced by either treatment these results may suggest that the BB MA systems different from their ability to inhibit control of PTZ seizures act to potentiate lidocaineinduced convulsions | MEDAL |
a cdna encoding human urokinase was inserted into the autographa californica nuclear polyhedrosis virus acnpv genome at the polyhedrin gene locus under control of a duplicated copy of the late basic MRP promoter the insectderived urokinase was produced predominantly in the form of singlechain prourokinase with a molecular mass of kda and demonstrated fibrinolytic activity synthesis and secretion of urokinase was first detected at hours postinfection and continued steadily throughout the infection period comparisons with urokinase synthesised using the very late acnpv polh promoter revealed that although the polyhedrin promoter is intrinsically stronger the yield of secreted urokinase was higher using the basic protein gene promoter these data support the hypothesis that the host cell sec pathway is compromised in the very late stages of baculovirus infection and may provide an explanation for why in general secreted and membranetargeted proteins are not produced to the high C2 observed with other proteins when using very late baculovirus gene promoters | MEDAL |
disk diffusion susceptibility testing for leptospira spp on leptospira vanaporn wuthiekanun lvw solid agar was reported recently however it was unclear whether the zone sizes obtained on lvw agar were comparable with those of other bacteria on muellerhinton agar | MEDAL |
the antithrombogenic EC cell defense atecd describes the properties that enable the endothelium to prevent circulating blood platelets from adhering to or aggregating on the vascular wall atecd was investigated in an experimental model in which bovine passage cultured EC cells ec were incubated with aggregating platelets and autologous plasma in a computeroperated aggregometerlike device a maximal PLT aggregation required x m adenosine diphosphate adp to be present in ecs a minute coincubation for ecs and platelets was found to be adequate in evaluating the maximal atecd value by increasing the ec number in the aggregation suspension PLT aggregation was progressively inhibited through a sigmoid curve inhibition of aggregation required x ec pharmacologic modulations of atecd by isosorbide dinitrate isdn isosorbide mononitrate ismn isosorbide mononitrate ismn were investigated under experimental conditions reflecting either an acute nitrate effect platelet control ecs drug adp or a chronic effect platelet day nitratetreated ecs adp under acute circumstances isdn antiplatelet activities were profoundly magnified by ecs aggregation was fully arrested with x m isdn and an ec number of x whereas the same isdn concentration CT induced inhibition of control aggregation in contrast there were no significant changes in platelet aggregation whether incubation was done in the presence or absence of ismn or ismn isdn metabolites longterm SE of ecs to isosorbide nitrates isn resulted in increased acquired ec changes in atecd ismn was a poor antiplatelet agent but was capable of counteracting isdn effects on atecdabstract truncated at words | MEDAL |
the aim of this study was to evaluate our results of RF catheter ablation rfca of IVT | MEDAL |
diversion of HPB away from the liver in the portal vein ligated pvl male rat results in dysfunction of the hypothalamopituitarygonadal axis as reflected by an increase in circulating oestradiol a decrease in ST and decreased expression of the malespecific cytochrome p c in hepatic microsomes the present study assessed whether there was a decline in the hepatic concentrations of mrna species corresponding to malespecific ps and whether femalespecific hepatic enzymes may be upregulated T3 pvl in microsomes from pvl male rat liver the activities of ps c and a androstenedione alpha and betahydroxylation respectively were decreased to p and p of control slot blotting revealed a decline in the mrnas for p c and a to and of respective control relative to betaactin mrna in contrast mrnas for the female specific p c and delta ketosteroid alphaoxidoreductase were unchanged from control and respectively p c protein was not detected in either control or pvl male rat CL but a slight increase in the rate of alphadihydrotestosterone formation was noted after pvl vs nmolminmg protein p corresponding respectively to and of the activity in female liver microsomes these studies indicate that the malespecific p c and a are downregulated at a pretranslational level whereas upregulation of the femalespecific enzymes p c and delta ketosteroid alphaoxidoreductase does not occur after pvl thus the endocrine effects of portal bypass result in biochemical demasculinization but not feminization of hepatic enzymes involved in steroid biotransformation because malespecific ps are ERP steroid hydroxylating enzymes their down regulation after pvl would significantly decrease hepatic androgen SE | MEDAL |
chronic inflammatory demyelinating polyneuropathy is a rare autoimmune disorder characterized by chronically progressive or relapsing symmetric sensorimotor involvement we describe the imaging findings in our patient magnetic resonance imaging showed presence of an intracranial white matter lesion and enhancing thickened cranial and spinal SN this disorder has been described very infrequently in the radiology literature | MEDAL |
recent data from several large studies of pediatric BPI reported baseline current episode duration ranging from less than a month to or year these data may reflect actual sample differences but the absence of uniformly applied definitions of episode duration number of lifetime episodes and daily rapid cycling patterns during episodes may also account for these differences | MEDAL |
the following hemodynamic parameters were determined in anesthetized mongrel dogs heart rate systolic blood pressure in the ascending aorta left VVI pressure at the peak dpdt left ventricular EDV pressure time intervals from q in ecg to peak dpdt maximum rate of left ventricular pressure rise FA arterial flow and certain indices of left ventricular +dP/dt it was demonstrated that prostacyclin even in high doses has no significant effect on hemodynamic parameters in dogs | MEDAL |
an oxygenstabilized enzyme electrode was applied to monitor the glucose concentration in a fermentor during a batch SC ofcandida utilis the electrode contains an electrolysis circuit for generation of oxygen within the enzyme layer that keeps the oxygen activity in that SL at the same level as that of the surrounding broth the electrolysis current is used as a measure of the gl concentration in the broththe gl analysis continued without L1 disturbances when the dissolved oxygen pressure gradually decreased during the fermentation and also when the broth was subjected to a sudden increase in DOT the electrode could also be used in an anaerobic BB then the reference electrode was replaced by a constant REF potential that simulated a REF oxygen activity | MEDAL |
the incidence of coronary artery disease cad is greater in men than in women the aim of the T0 was to analyze whether any genderrelated differences in patients with cad and documented spontaneous sustained VVI tachyarrhythmias exist and which parameters influence the induction of sustained VTs the data of patients women and men with spontaneous sustained IVT n and fibrillation n who underwent coronary and left ventricular angiography EP study and signalaveraging electrocardiogram ecg form the basis of this analysis no genderrelated differences could be observed in age number of diseased CAs history location and number of myocardial infarctions presence of left VVI aneurysm ejection fraction type of spontaneous or induced arrhythmias RA VVI ERP refractory period and signalaveraged ecg parameters age presence of previous myocardial infarction and ejection fraction were significant predictors p of inducibility of sustained ventricular tachyarrhythmias once cad has begun female and male patients present similar clinical and EP characteristics thus both genders should benefit similarly from diagnostic and therapeutic approaches if they are referred to the hospital or to invasive interventions at similar intervals in the course of their illness | MEDAL |
chick CL cell monolayers synthesize fatty acids at in vivo rates and are responsive to insulin and glucagon high rates of HFA synthesis are maintained with insulin present and lost slowly without insulin glucagon or cAMP cause immediate cessation of HFA synthesis the site of inhibition appears to be cytoplasmic acetylcoa carboxylase which catalyzes the first committed step of fatty acid synthesis liver carboxylase exists either as catalytically inactive protomers or AS filamentous polymers citrate an allosteric activator of the enzyme is required for both catalysis and polymerization glucagon and camp cause an immediate decrease in the cytoplasmic citrate concentration of chick CL cells apparently by inhibiting the conversion of glucose to citrate at the phosphofructokinase reaction since HFA synthesis and citrate level are closely correlated citrate appears to be a feedforward activator of the carboxylase in vivo compelling evidence indicates that carboxylase filaments are present in the intact cell when citrate C2 are high and depolymerize when citrate C2 fall hence carboxylase activity and HFA synthetic rate appear to be determined by cytoplasmic citrate level | MEDAL |
cardiac sac network activity cycle period tens of seconds to T2 has long been known to alter pyloric network activity cycle period approximately s but these effects have not been quantified some pyloric muscles extract cardiac sac timed variations in pyloric motor neuron firing and consequently produce cardiac sac timed movements even though no cardiac sac SN innervate them determining pyloric SMB therefore requires detailed description of cardiac sac effects on pyloric neural output pyloric muscle activity correlates well with motor neuron overall spike frequency osf number of spikes per burst divided by cycle period we therefore quantified the effects of cardiac sac activity on the osf of all pyloric SN in the lobster panulirus interruptus the VD vd neuron had a biphasic response with its osf first increasing and then decreasing during cardiac sac bursts lateral pyloric lp neuron osf decreased during cardiac sac activity the pyloric py neurons had two responses with osf either decreasing or increasing just after the beginning of cardiac sac activity the pyloric dilator pd SN had a triphasic response with osf increasing slightly at the beginning of cardiac sac activity decreasing during the cardiac sac burst and strongly increasing T3 cardiac sac activity ended the IC ic neuron had a biphasic response with osf decreasing at the beginning of cardiac sac activity and strongly increasing when cardiac sac activity ceased these data provide the quantitative description of cardiac sac effects on pyloric activity necessary to predict pyloric movement from pyloric neural output | MEDAL |
application of bradykinin bk to the spinal cord of the neonatal rat evoked depolarizations which could be recorded via either the dorsal or ventral roots however responses recorded via the ventral root were abolished by removal of extracellular ca or the addition of cd while responses recorded via the dorsal root were unaffected the response recorded via the VR was inhibited by the substance p antagonist spantide while responses recorded via the dorsal root were unaffected depolarizations recorded via the dorsal root were concentrationdependent with an ec of nm these responses were not antagonized by the bk selective antagonist leudesargbk but were antagonized by darghypthidphebk with a pa of which is similar to the values determined for other bkmediated responses application of phorbol dibutyrate pdbu to the spinal cord also evoked a depolarization with respect to the dorsal root this response to pdbu was enhanced by removal of extracellular ca while the response to bk was unaffected the potent protein kinase inhibitor staurosporine reduced the response to pdbu but did not affect the response to bk these results suggest that bk by acting on bk receptors can depolarize the central terminals of primary afferent nerve fibres this response to bk does not appear to be mediated via the activation of protein kinase c the depolarization to bk recorded via the ventral root of the spinal cord is indirect and may be secondary to the action of bk on the primary afferent terminals | MEDAL |
we investigated fat CSD and tissuespecific insulinstimulated gl uptake gu in seven fat compartments visceral and subcutaneous and skeletal muscle in morbidly obese patients with td and without nd type diabetes before and months after bariatric surgery | MEDAL |
immunocytochemistry using antibodies against the neurohypophysial nonapeptides has given equivocal results regarding relevant aspects of the CP NS system of elasmobranchs the lack of antibodies reacting with the elasmobranch neurophysins nps has prevented the study of this neurosecretory system by nps immunocytochemistry this led us to purify nps from scyliorhinus canicula and to use them to raise a polyclonal antibody this antibody reacted strongly with the elasmobranch NP neurons revealing their most delicate and distant hypophysial and extrahypophysial projections a detailed mapping of the NS system of five elasmobranch species etmopterus spinax squalus acanthias scyliorhinus canicula galeus melanostomus raja radiata and one holocephalian species hydrolagus colliei was performed in elasmobranchs the magnocellular neurophysin cells formed a distinct PON whereas in hydrolagus the immunoreactive cells were scattered distinct parvicellular neurophysin cells were present in the PON in raja the nucleus o contained parvicellular npsimmunoreactive SN the findings at the PIT level point to the possibility that NP neurons in addition to releasing nonapeptides into the SVR capillaries of the neural lobe also participate in the regulation of the CF of the rostral medial and intermediate lobes of the adenohypophysis by a dual mechanism ie a neurovascular pathway and a direct neural input the extrahypophysial projections of the NP SN were highly developed to a degree not comparable to any other vertebrate group the targets of these projections were located in the telencephalon diencephalon and hindbrain the evolutionary and functional implications of this phenomenon are discussed | MEDAL |
quality of life qol was evaluated in patients having radical gastrectomy for CA the results showed that more than half of the patients had a good appetite they consumed a normal diet and a normal volume of food approximately of the patients had weight loss of more than kg patients who underwent a TG had poor tolerance of normal food and frequent eating and body WL versus those who had a subtotal gastrectomy patients who underwent BII reconstruction T3 a distal SG lost more body weight than those with a billroth i anastomosis the extent of lymphadenectomy did not influence the qol patients under years of age had a better qol nearly all patients had normal work and daily living activities some patients appeared to lack energy or had a period of anxiety or depression these data indicate that radical gastrectomy can be performed with an acceptable qol for a potentially curable gastric carcinoma | MEDAL |
some human malignant lymphomas of the bcell type have morphologic and immunologic similarities to follicles seen in nonneoplastic reactive CL nodes in contrast a peculiar vaguely nodular DP of growth called pseudonodules or pseudofollicular proliferation centers which is morphologically distinguishable from true follicles is seen in malignant lymphoma welldifferentiated small lymphocytic type wdl to characterize the cellular components of pseudofollicles we undertook a detailed comparative immunohistologic study of the architectural relationship and CSD of t cells B1 and follicular dendritic reticulum cells drcs in reactive follicles neoplastic follicles and pseudofollicles we report several observations on the presence of drcs and tcell subset topography in pseudofollicles immunohistologic IF for the cd CR1 on drcs revealed that drc networks associated with true follicles were present in all cases of reactive follicular PH rfh and malignant lymphoma nodular poorly differentiated lymphocytic type pdl studied surprisingly drc networks were also identified in of cases of wdl although the size distribution of drc network diameters was nearly MZ in rfh and pdl the sizes were markedly diminished in wdl immunohistologic staining for leu and leu tcell subsets confirmed cellular arrangements in rfh and pdl reported by others in only of cases of wdl could TA localized to pseudofollicles in frozen tissue sections be identified in a nonrandom arrangement | MEDAL |
the paper presents the surgical technique for kramers distal i metatarsal osteotomy and the results of halluces valgi treatment in patients the mean age of the patients was years and mean followup time was years and months final clinical and radiological evaluation was performed according to kramers scale excellent and good results were found in of the cases fair in and bad in this simple extraarticular osteotomy without use of any external immobilization yielding threedimensional correction is a useful method for treatment of hallux valgus | MEDAL |
we analyzed the cellcell adherence related to cdcd and cd mrna in individuals with decreased cdcd expression on their neutrophil surface epstein barr virustransformed b cell lines were developed from one localized juvenile periodontitis ljp patient with decreased cdcd in the IP blood neutrophils and without systemic diseases two siblings with generalized PP gpp caused by LAD lad another ljp patient one localized prepubertal periodontitis lpp patient and two healthy subjects adhesion of leukocytes to each other was measured as cluster formation by aggregation CA the length and the amount of cd mrna expressed in the cell lines were analyzed by RNA blotting using the plabeled cd cdna the coding region of the mrna was analyzed by the reverse transcriptionpolymerase chain reaction method basemismatches between cd mrna and the plabeled rna probe synthesized from cd cdna were analyzed by rnase RPA in the adherence assay cells from the ljp patients with decreased cdcd formed more clusters of smaller size and fewer cells than those of the other subjects the cells from gpp and lad patients did not aggregate and did not form clusters either in the absence or presence of pma there were no differences in the length and the amount of mrna between the ljp patients and the other subjects while gpplad patients expressed a small amount of long mrna the whole coding region base pairs of all subjects was amplified except for the gpplad patients and the region base pairs was amplified from all subjectsabstract truncated at words | MEDAL |
twentyeight mycotoxins were studied in the hepatocyte primary culturedna repair test using rat and mouse HCs dna repair synthesis was elicited by several EDC of unknown carcinogenicity dimethoxysterigmatocystin versicolorins a and b averufin xanthomegnin luteosporin and chrysazin as well as by the carcinogenic myocotoxins aflatoxin b sterigmatocystin luteoskyrin ochratoxin a azaserine mitomycin c and actinomycin d the positive results with compounds of unknown carcinogenicity suggest that they are possibly genotoxic carcinogens the carcinogenic mycotoxins penicillic acid patulin griseofulvin and rugulosin which did not elicit repair synthesis may be nongenotoxic carcinogens | MEDAL |
for many hong kong chinese elderly depression and financial strain are quite a common occurrence this study examines the mediating and moderating effect of sense of control in the relationship between financial CS and DS the data came from a survey of a representative community sample of elderly respondents in hong kong using multiple regression models the authors found that generalized sense of control mediated and moderated the linkage between financial strain and depressive symptoms even T3 controlity of surgery in patients with C2 cancerranging between modified RH and radical hysterectomy with resection of the parametria to both pelvic side walls assessment of lymph node status is mandatory for individual treatment pelvic lymphonodectomy represents ST at radical hysterectomy and if positive paraaortic lymphonodectomy is indicated micturition disorders urinary incontinence CL cysts bowel problems thrombophlebitis urogenital fistulas and pulmonary embolism are the most frequent complications following RH today there is an increasing competition between different therapy strategies in cervical cancer such as primary irradiation chemoradiation neoadjuvant chemotherapy laparoscopic RH vaginal RH combined with laparoscopic surgical staging all these treatment modalities have to be compared with the golden standard comprising survival and recurrence rates functional results morbidity mortality and SQ of life | MEDAL |
myocardial perfusion imaging mpi is an important procedure in pediatric cardiology in terms of evaluating MI infarction and damage associated with various congenital or acquired HR diseases such as kawasaki disease anomalous origin of the left PD from the pulmonary artery and CR TGA after arterial switch surgery this type of imaging can detect myocardial damage in the morphological right ventricle when it functions as a systemic pumping chamber in patients with complex congenital heart diseases after intracardiac repair MPI can also evaluate myocardial damage associated with primary or AA cardiomyopathy in children the magnitude of increased RA ventricular uptake on mpi is a useful noninvasive means of estimating right ventricular pressure overload due to congenital heart or pulmonary diseases this article reviews myocardial perfusion tracers and pharmacological stress tests used to diagnose HR conditions in children and the current clinical roles of mpi in pediatric cardiology | MEDAL |
fecal coliform bacteria are used as indicator organisms for the presence of pathogens in sludges it has often been assumed that the counts of fecal coliforms T3 digestion where the sludges may also be called biosolids are representative of the counts when the sludge is disposed or recycled such as by land application the possibility has been raised however that dewatering processes can lead to increased counts of fecal coliforms and by inference human pathogens this paper presents data from previous studies of this possibility the results were inconsistent but showed observable increases in fecal coliforms at one treatment plant additional studies were then performed at another MTF which showed statistically significant increases in FC counts after dewatering and two days of aging the increases exceeded two orders of magnitude and included two centrifuge types and two biosolids types artifacts of media type and enumeration method have been excluded and shearing of the material by commercial blender did not produce the same effects | MEDAL |
concussion guidelines recommend physical and cognitive rest until all PCS resolve in part because of potential health risks including catastrophic injury related to a second impact however when postconcussion symptoms persist for weeks or months these risks are poorly characterized | MEDAL |
a yearold man with HCC carcinoma associated with alcoholic cirrhosis was hospitalized for lightheadedness and melena he had undergone multiple surgeries and had been treated with transcatheter arterial chemoembolization and sorafenib neither upper nor lower gastrointestinal endoscopy detected the source of one oral doubleballoon enteroscopy revealed a mass lesion in the upper jejunum cm from the treitz AL on the anal side which was identified as the source of bleeding subsequently a biopsy was performed a histopathological examination detected a hepatocellular carcinoma and a final diagnosis of jegment and recovery occurs within two months past reports have shown that in three of seven kd cases with NVD involvement optic disc swelling lasted over two months this is the second case in which the condition lasted six months | MEDAL |
the present study was performed to examine the mechanism by which cyclooxygenase blockade produces vasoconstriction in welldeveloped coronary collateral vessels eight dogs were studied to months T3 occlusion of the LAD i.a. lad had been performed to stimulate collateral vessel growth at the time of T0 the lad was cannulated at the site of occlusion for measurement of VA blood flow as an index of collateral blood flow levels of ketoprostaglandin f alpha were higher in blood diverted from the collateraldependent lad than in aortic blood p the increase in this SD product of prostacyclin metabolism indicated production of prostacyclin across the coronary collateral system administration of AA into the left main coronary artery to reach collateral vessels entering the lad resulted in a increase in retrograde flow p demonstrating cyclooxygenase activity with production of vasodilator prostaglandins in the collateral system ibuprofen mgkg iv caused a decrease in VA flow p suggesting that cyclooxygenase blockade inhibited tonic production of vasodilator prostaglandins in the Rcs in contrast neither thromboxane synthase inhibition with dazmegrel nor TP blockade with sq caused a significant change in collateral flow thus failing to support thromboxaneinduced collateral constriction T3 cyclooxygenase blockade prostacyclin infused into the LM coronary artery was able to restore retrograde flow to the preibuprofen levelabstract truncated at words | MEDAL |
therapyrelated changes of the bone marrow fiber content remain a controversial issue in hematopathology this conflict of opinion firstly depends on difficulties to determine the quantity of SF exactly semiquantitative grading morphometry reference to cellularity secondly the appropriate selection of patients with specific monotherapies including hydroxyurea hu and interferonalpha ifn seems to present some problems finally assessment of myelofibrosis is further biased by the different endpoints of sequential examinations the latter shortcoming can be improved upon by the calculation of the myelofibrosis progressionregression index which describes the ratio between difference of fiber density and observation time using strictly defined therapeutic regimens and intervals between sequential trephine biopsies a stimulating effect of ifn administration on bone marrow fibrosis in phchronic myelogenous one cml has been found this result is comparable with the failure of this agent to improve myelofibrosis and splenomegaly in a considerable number of patients with allied subtypes of chronic myeloproliferative disorders this is in contrast to the effect hu exerts which is a more fibrolytic or even stabilizing influence on bone marrow fibrosis this phenomenon is readily demonstrable by the assessment of dynamic features myelofibrosis progression index in addition patients showing a rapid progression of myelofibrosis during ifn and hu treatment of phcml are generally associated with a poor risk outcome and a significant worsening of survival | MEDAL |
the objective of this T0 was to determine the risk factors of erectile dysfunction ed for patients entering the dialysis programme with haemodialysis hd or PD pd participants were adult males with sexual partners they were given the semiquantitative standard international index of EF questionnaire we added the common risk AF in specially designed questions to cover our objectives the data were analysed using a multivariate regression MM fiftytwo patients fulfilled the eligibility criteria and completed this questionnaire twentyfive were on hd and on pd only were potent on entry into the T0 among the rest were classified under severe ed sexual desire appeared less affected in comparison to other domains although expressed their interest in treatment for ed none of the patients received any stepwise selection analysis identified the only significant risk factor to be older age there was no difference between the two populations of hd and pd it is concluded that ed is very prevalent in men beginning dialysis it should be assessed and treatment be offered | MEDAL |
an antiarrhythmic treatment was done in patients with recurrent VVI tachycardias using amiodarone the dosage was to mgd following a loading dosage of mg for to days amiodarone and desethylamiodarone concentration in SS control group n and in erythrocyte haemolysate control group n were determined in relapses of VVI tachycardias n and in PF as serious side effect n it was shown that amiodarone C2 rise continuously during loading treatment until the th to th day and that desethylamiodarone can be demonstrated T3 the rd day of treatment the mean concentrations sigma of amiodarone and desethylamiodarone were microgramml and microgramml in SS and microgramml and microgramsml in EA haemolysate amiodarone C2 did not correlate with efficacy and with incidence of side effects however in pulmonary fibrosis high desethylamiodarone concentrations in SS greater than microgramsml and in erythrocyte haemolysate greater than microgramsml were found four out of patients with recurrent VVI ART showed relatively low desethylamiodarone concentrations in serum desethylamiodaroneamiodarone ratio less than thus control of amiodarone treatment can be enlarged by determination of desethylamiodarone C2 as its concentrations correlate with relapses of ventricular tachycardias and serious SE | MEDAL |
the aim of this T0 was to propose a method to predict the minimum achievable mean lung dose mld and corresponding dosimetric parameters for organsatrisk oar based on individual patient anatomy for each patient the dose for equidistant individual MLC shaped fields in the treatment planning system tps was calculated based on these dose matrices the mld for each patient was predicted by the homemade dosepredictor software in which the solution of linear equations was implemented the software prediction results were validated based on d conformal radiotherapy dcrt and volumetric modulated arc therapy vmat plans previously prepared for patients with stage iii nonsmallcell LC nsclc for each patient dosimetric parameters derived from plans and the results calculated by dosepredictor were compared the mld the maximum dose to the spinal cord d | MEDAL |
we propose a novel method for detecting CS of molecular recombination in multiple alignments our approach is a compromise between previous extremes of computationally prohibitive but mathematically rigorous methods and imprecise heuristic methods using a combined algorithm for estimating tree structure and hidden markov MM parameters our program detects changes in phylogenetic tree topology over a multiple sequence alignment we evaluate our method on benchmark datasets from previous studies on two recombinant pathogens neisseria and hiv as well as simulated data we show that we are not only able to detect recombinant regions of vastly different sizes but also the location of breakpoints with great accuracy we show that our method does well inferring recombination breakpoints while at the same time maintaining practicality for larger datasets in all cases we confirm the breakpoint predictions of previous studies and in many cases we offer novel predictions | MEDAL |
the incidence of cardiac arrhythmias is estimated et per pregnancies usually in the third trimester and of them are asymptomatic they may appear for the first time in pregnancy or have a recurring character an important risk factor related to their appearance is the presence of structural heart disease which complicates of pregnancies generally the PS are mild and the treatment is not necessary but in some cases pharmacotherapy is necessary pharmacotherapy must be a compromise between the potentially adverse effects of drugs on the fetus and the beneficial effects on the cardiovascular system of the mother due to the OD of cardiac surgery many women with heart defects reach reproductive age and become Pr therefore this problem will be faced more and more often in clinical practice in addition to pharmacological methods some cardiac arrhythmias may require urgent lifesaving procedures external electrical cardioversion is associated with the application of certain amount of energy via two electrodes placed on the thorax it is used to treat hemodynamically unstable SVT tachycardias including atrial fibrillation and atrial flutter also in hemodynamically SD patients in whom drug therapy was ineffective elective electrical cardioversion can be use to convert cardiac arrhythmia to SR we present a case of a years old patient with congenital heart disease surgically corrected in childhood who had first incident of atrial flutter in pregnancy arrhytmia occured in th week of gestation the patient was hemodynamically stable and did not approve electrical cardioversion as a method of treatment therefore pharmacotherapy was started heart rate was controled with metoprolol and digoxin warfarin was used to AC calcium and potassium were also given described therapy did not convert atrial flutter to sinus rhythm therefore in rd week of gestation after patients approval electrical cardioversion was performed before cardioversion transesophageal echocardiogram was made to exclude the presence of thrombus inside atria SE of j was applied and sinus rhythm was restored cardiotocography during and after cardioversion did not show any significant fetal heart rate changes further pregnancy and puerperium were uneventful case report and review of the literature about cardiac arrhytmias and methods of its treatment especially in Pr women analysis of medical documentation of the patient treated in the department of cardiology as well as in the department of obstetrics and perinatology medical university of lublin review of abstracts and papers in the medline DB about heart arrhytmias occuring during pregnancy methods of their treatment with special refference to electrical cardioversion pregnancy is a condition which predisposes to cardiac arrhytmias it is associated with changes in CVS of Pr women that appear physiologically they can be effectively treated with low risk for mother and fetus electrical cardioversion is an ERP and safe method of therapy of SVT arrhytmias also during pregnancy the adaptation of the maternal hemostasis in pregnancy predisposes women to an increased risk of TE therefore ACT is essential to minimize the risk of embolic episodes and stroke during elective cardioversion pregnant women with structural or functional HR diseases should be under supervision of multidiscyplinary team of specialists obstetricians cardiologists neonatologists pediatricians | MEDAL |
the tau of sucrose as a flavor for activated charcoal was studied in vitro adsorption of sucrose in simulated gastric fluid usp without pepsin to AC and of a gliter sodium salicylate solution to a mixture of sucrose and AC and to plain AC was measured spectrophotometrically in vitro adsorption of sucroses to AC was minimal sucrose reduced in vitro adsorption of sodium salicylate to G1 charcoal by only small amounts for example at a ratio of g activated charcoal to g sodium salicylate SU reduced salicylate adsorption to activated charcoal from to a sucroseactivated charcoal preparation provides sufficient flavor without substantial loss of adsorbance | MEDAL |
despite typically not being taken into account usually in favour of the global distillation process the input of POPs pops through biological activities can be indeed relevant at the local scale in terrestrial polar environments when seabird colonies are considered seabirds can bioaccumulate and biomagnify pops gather in C1 numbers and excrete on land during their reproductive season thus making them locally as relevant secondary sources of pops the first part of this T0 indicated that these colonies act as so for several essential and nonessential trace elements and this second part tests the same hypothesis concerning pops using the very same samples lichens n mosses n and soil n were collected from locations in the south shetlands archipelago during the austral summers of and they were divided in colony within the colony itself for soil and within and surrounding the colony for vegetation and control at least m away from any colony interference and analysed for pops such as organochlorine pesticides polychlorinated biphenyls pcbs and polybrominated diphenyl ethers and stable isotopes c and n results showed that colonies act clearly as a secondary source for pcbs and likely for hexachlorobenzene as in the first part probable local sources other than the colonies themselves are hypothesised because of high concentrations found in control sites again soil seemed the most adequate matrix for the intended purposes especially because of some particularities in the absorption of animalderived organic matter by vegetation pointed out by stable isotope analyses | MEDAL |
protoncoupled electron transfer pcet at metaloxide nanoparticle interfaces plays a critical role in many photocatalytic reactions and energy conversion processes recent experimental studies have shown that photoreduced zno NCs react by pcet with organic hydrogen atom acceptors such as the nitroxyl radical tempo herein the interfacial pcet rate constant is calculated in the framework of vibronically nonadiabatic pcet theory which treats the electrons and transferring proton quantum mechanically the input quantities to the pcet rate constant including the electronic couplings are calculated with DFT the computed interfacial pcet rate constant is consistent with the experimentally measured value for this system providing validation for this pcet theory in this MM the electron transfers from the conduction band of the zno nanocrystal to tempo concertedly with proton transfer from a surface oxygen of the zno nanocrystal to the oxygen of tempo moreover the proton tunneling at the interface is gated by the relatively lowfrequency proton donoracceptor motion between the tempo radical and the zno nanocrystal the zno nanocrystal and tempo are found to contribute similar amounts to the innersphere reorganization energy implicating structural reorganization at the nanocrystal surface these fundamental mechanistic insights may guide the design of metaloxide nanocatalysts for a wide range of SE conversion processes | MEDAL |
the localization of transcribing rrna genes within nucleoli of mammalian cells although intensively studied has not been established most published papers on this topic situate transcribing ribosomal genes either to nucleolar FCs or to nucleolar dense fibrillar components to clarify this point we have generated the electron microscopic affinity cytochemistry picture of the nucleolus of cultured rat cells three kinds of affinity probes have been used probes to nucleolar chromatin including rdna sequences probes to a number of macromolecules such as rna polymerase i which are directly or indirectly involved in the synthesis and processing of rrna and formation of preribosomes antibodies to bromouridine for a recently standardized nonisotopical method depicting incorporated bromouridine within rna the results suggest the localization of transcription CS not only to dense fibrillar components but also to the border region between these components and fibrillar centers our data support a hypothesis that in metabolically AS mammalian nucleoli FCs and dense fibrillar components form a single PET domain for the transcription of rrna genes with nascent transcripts generating automatically dense fibrillar components through the AS process of transcription individual rrna genes thus become engulfed within dense fibrillar components | MEDAL |
the role of blood viscosity and haemostasis has been investigated in mixed groups of patients with branch and central retinal vein occlusion crvo with conflicting results this may have partly been due to the different aetiologies of these two types of vein occlusion | MEDAL |
prostaglandin e pge plays a key role in the maintenance of human pregnancy and labour onset pge can elicit diverse actions within the uterus depending on the EP ep ep ep and ep expressed by signalling through different intracellular pathways the ep receptors may inhibit or promote smooth muscle +dP/dt nine different ep receptor SVs have been identified with divergent signalling pathways rtpcr and immunohistochemistry were utilized to identify and localize ep receptor isoforms within the upper segment us and lower segment ls myometrium ep was significantly increased in the ls myometrium with term labour ep and ep splice variants epib epii epiii and epiv was downregulated in pregnancy us andor ls with a further decrease at term labour in the ls overall expression of ep was significantly higher in the ls while ep was significantly higher in the us no significant ep changes were observed consistent with the rtpcr results immunohistochemistry confirmed the presence and interestingly showed nuclear localization of ep receptors in the myometrium with higher ep expression and lower expression of ep the differential regulation of ep receptors within the myometrium indicates that they may play a role in controlling the onset and maintenance of human labour | MEDAL |
objectives picrosirius red and mmpsures also result in a decrease in testicular testosterone in rats the antiandrogenic effects of phthalates have been more thoroughly quantified than testicular pathology due to the significant time requirement associated with manual counting of mngs on histological sections an automated counting method was developed in imagej to quantify mngs in digital images of hematoxylinstained rat fetal testis tissue sections timed pregnant sprague dawley rats were exposed by daily p.o. from gestation day to with one of eight phthalate test compounds or CO VH both the manual counting method and the automated image analysis method identified dinbutyl phthalate butyl benzyl phthalate dipentyl phthalate and diethylhexyl phthalate as positive for induction of mngs dimethyl phthalate diethyl phthalate the brominated phthalate diethylhexyl tetrabromophthalate and dioctyl terephthalate were negative the rho between automated and manual scoring metrics was high r results of mng analysis were consistent with these EDC antiandrogenic activities which were confirmed in an ex vivo testosterone production assay in conclusion we have developed a reliable image analysis method that can be used to facilitate doseresponse studies for the reproducible induction of mngs by in utero DHP exposure | MEDAL |
because porcine chromosome ssc has become the focal point of many efforts aimed at identifying quantitative trait loci affecting or genes distributed across HSA hsa were physically mapped in the pig a more refined comparative map of this region for these two species was produced in this T0 four genes were selected based on their location in the human genome the availability of nucleotide CS and their genomic organization the genes selected were fibroblast growth factor basic fgf hsa q gonadotropin releasing hormone receptor gnrhr hsa q phosphodiesterase b pdeb hsa p and aminopeptidase s peps hsa pq genomic libraries were screened via pcr and clones were physically assigned using fluorescence in situ hybridization fish these four genes from hsa were physically mapped to ssc p pdeb p peps q gnrhr and q fgf these assignments provide additional benchmarks for the comparative map and help define the level of gene order conserved between hsa and ssc | MEDAL |
catch syndrome caused by a microdelection in chromosome is characterized by cleft palate and cardiac anomalies the majority of these children also have learning difficulties or speech and language deficits these problems are often due to the dysmorphology of the articulatory system in the present study the duration of auditory sensory SM which is of CE importance to speech perception and understanding was investigated as a research method we used mismatch negativity mmn an attention independent eventrelated potential which provides an objective electrical index of AEP sensory memory the present data suggest that the duration of this memory span is considerably shorter in yearold children with catch than in HCs thus the languagerelated problems encountered in children suffering from catch syndrome are likely to be caused also by cns dysfunctions | MEDAL |
PCTs are increasingly being conducted as new products seek to enter the market deployment of such interventions is based on evidence obtained mainly from the gold standard of randomized controlled clinical trials rcct a crucial factor in the ability of rccts to provide credible and generalisable data is sample size and PR of the required number of subjects at completion of the followup period however recruitment and retention in PCTs are hindered by prevalent peculiar challenges in africa that need to be circumvented this article shares experiences from a phase ii trial that recorded a high retention rate at months followup at a new clinical trial site | MEDAL |
metabolomics data provide unprecedented opportunities to decipher metabolic mechanisms by analyzing hundreds to thousands of metabolites data quality concerns and complex batch effects in metabolomics must be appropriately addressed through statistical analysis this T0 developed an integrated analysis tool for metabolomics studies to streamline the complete analysis flow from initial data preprocessing to downstream association analysis we developed statistical metabolomics analysisan r tool smart which can analyze input files with different formats visually represent various types of data features implement peak alignment and annotation conduct quality control for samples and peaks explore batch effects and perform association analysis a pharmacometabolomics study of antihypertensive medication was conducted and data were analyzed using smart NMN was identified as a metabolite significantly associated with angiotensinconvertingenzyme inhibitors in our metabolomewide association analysis p à in an analysis of covariance ancova with an adjustment for unknown latent groups and p à in an ancova with an adjustment for hidden substructures this endogenous neuropeptide is highly related to NT and neuromedin u which are involved in blood pressure regulation and smooth muscle contraction the smart software a user guide and example data can be downloaded from httpwwwstatsinicaedutwhsinchoumetabolomicssmarthtm | MEDAL |
amineptine and its main metabolite were determined simultaneously in plasma by highperformance liquid chromatography using quinupramine as internal standard the method comprised adsorption on extrelut column from alkaline plasma elution with diethyl ethermethylene chloride evaporation in the presence of m hydrochloric acid and injection of the acid solution onto a mu bondapak c column using acetonitrile m potassium dihydrogenphosphate as mobile phase and ultraviolet PCD at nm average steadystate concentrations of the two compounds were determined in four patients under treatment regimen two mg doses of amineptine per day at and h the concentrations determined h after the last dose were undetectable in all cases whereas the concentrations determined h after the second dose were found to be ng ml for amineptine and ng ml for its metabolite the technique can also be applied to whole blood with if necessary identification on the basis of the ultraviolet spectrum obtained by photodiodearray detection | MEDAL |
bone marrow bm samples fromajority of patients was by simple closure or truncal vagotomy and pyloroplasty an increase in patients awareness of the potentials of optimal operative management should encourage earlier presentation | MEDAL |
multiple sclerosis ms is a neurological disease with a variety of signs and PS exercise therapy has been shown to improve physical functions in ms however questions about an optimal exercise therapy remain in this regard we suggest a combined exercise therapy including aerobic and resistance exercises for ms patients the study is designed to observe test and compare the effects of proposed combined exercises on strength balance agility fatigue speed and walking distance in people with mild to moderate ms expanded disability status scale edss | MEDAL |
GBS causes a variety of morbid and sometimes fatal conditions affecting individuals of all age groups there are nine known serotypes of this GP coccus but few estimates of the incidence and duration of its colonization and none by serotype in the literature in the authors conducted a prospective cohort T0 among men and women living in a single dormitory in ann arbor michigan the week incidence with any serotype was among women and among men week incidence rates were highest for serotype v for women and for men and type ia for women and for men with no significant differences by gender the estimated average duration of any group b streptococcus colonization was longer for women weeks than men weeks serotype ia was carried an average of weeks longer in women and serotype iii was carried weeks longer colonization with more than one serotype occurred significantly less than would be expected by chance p based on the overall incidence transmission occurred between roommate pairs at the rate expected group b streptococcus colonization is frequent and dynamic but it is not transmitted by casual contact | MEDAL |
ergot alkaloids cause fescue toxicosis when livestock graze endophyteinfected tall fescue it is generally accepted that ergovaline is the toxic component of endophyteinfected tall fescue but there is no direct evidence to support this hypothesis the objective of this T0 was to examine relative and potential transport of ergoline and ergopeptine alkaloids across isolated gastric tissues in vitro sheep ruminal and omasal tissues were surgically removed and placed in parabiotic chambers equimolar concentrations of lysergic acid lysergol ergonovine ergotamine and ergocryptine were added to a krebs ringer phosphate krp solution on the mucosal side of the tissue tissue was incubated in nearphysiological conditions for min samples were taken from krp on the serosal side of the chambers at times and min and analyzed for ergot alkaloids by competitive elisa the serosal krp remaining T3 incubation was FD and the alkaloid species quantified by hplc the area of ruminal and omasal tissues was measured and the potential transportable alkaloids calculated by multiplying the moles of transported alkaloids per square centimeter of each tissue type by the SA of the tissue studies were conducted to compare alkaloid transport in reticular ruminal and omasal tissues and to determine whether transport was active or passive ruminal tissue had greater ergot alkaloid transport potential than omasal tissue vs mmol because of a larger surface area the ruminal posterior dorsal sac had the greatest potential for alkaloid transport but the other ruminal tissues were not different from one another alkaloid transport was less among reticular tissues than among ruminal tissues transport of alkaloids seemed to be an AS process the alkaloids with greatest transport potential were lysergic acid and lysergol ergopeptine alkaloids tended to pass across omasal tissues in greater quantities than across ruminal tissues but their transport was minimal compared to lysergic acid and lysergol | MEDAL |
the aim of this work was to develop a microbioreactor using liquid marble lm as a novel system for oocyte in vitro maturation ivm in small volumes | MEDAL |
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