Delete test_protocol.py
Browse files- test_protocol.py +0 -48
test_protocol.py
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from opentrons import protocol_api
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metadata = {
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'protocolName': 'My Protocol',
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'author': 'Your Name',
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'description': 'Description of the protocol',
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'apiLevel': '2.12'
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}
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def run(protocol: protocol_api.ProtocolContext):
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# Labware
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plate = protocol.load_labware('corning_96_wellplate_360ul_flat', '1')
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tiprack = protocol.load_labware('opentrons_96_tiprack_300ul', '2')
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pipette = protocol.load_instrument('p300_single_gen2', 'right', tip_racks=[tiprack])
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# Parameters
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num_samples = 96
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sample_volume = 200
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reagent_volume = 50
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# Reagent reservoirs
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reagent_reservoir = protocol.load_labware('usascientific_12_reservoir_22ml', '3')
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# Transfer reagent to samples
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for well in plate.wells()[:num_samples]:
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pipette.pick_up_tip()
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pipette.aspirate(reagent_volume, reagent_reservoir.wells()[0])
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pipette.dispense(reagent_volume, well)
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pipette.blow_out(well.top())
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pipette.drop_tip()
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# Mix samples
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for well in plate.wells()[:num_samples]:
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pipette.pick_up_tip()
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pipette.mix(5, sample_volume, well)
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pipette.drop_tip()
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# Incubate samples
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protocol.delay(minutes=30) # Incubate for 30 minutes
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# Transfer samples to a new plate
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output_plate = protocol.load_labware('corning_96_wellplate_360ul_flat', '4')
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for src_well, dest_well in zip(plate.wells()[:num_samples], output_plate.wells()[:num_samples]):
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pipette.pick_up_tip()
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pipette.aspirate(sample_volume, src_well)
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pipette.dispense(sample_volume, dest_well)
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pipette.blow_out(dest_well.top())
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pipette.drop_tip()
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