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---
library_name: popV
license: cc-by-4.0
tags:
- biology
- genomics
- single-cell
- anndata_version:0.11.3
- python_version:3.11.11
- popV
- 'tissue: Large_Intestine'
---
Popular Vote (popV) model for automated cell type annotation of single-cell RNA-seq data. We provide here pretrained models
for plug-in use in your own analysis.
Follow our [tutorial](https://github.com/YosefLab/popV/blob/main/tabula_sapiens_tutorial.ipynb) to learn how to use the model for cell type annotation.
# Model description
Tabula Sapiens is a benchmark, first-draft human cell atlas of over 1.1M cells from 28 organs of 24 normal human subjects. This work is the product of the Tabula Sapiens Consortium. Taking the organs from the same individual controls for genetic background, age, environment, and epigenetic effects, and allows detailed analysis and comparison of cell types that are shared between tissues.
**Link to CELLxGENE**:
Link to the [data](https://cellxgene.cziscience.com/e/7357cee7-9f7f-4ab0-8cec-90de8f047e38.cxg/) in the CELLxGENE browser for interactive exploration of the data and download of the source data.
**Training Code URL**:
Not provided by uploader.
# Metrics
We provide here accuracies for each of the experts and the ensemble model. The validation set accuracies are
computed on a 10% random subset of the data that was not used for training.
| Cell Type | N cells | celltypist | knn bbknn | knn harmony | knn on scvi | onclass | scanvi | svm | xgboost | Consensus Prediction |
| --- | --- | --- | --- | --- | --- | --- | --- | --- | --- | --- |
| enterocyte of epithelium of large intestine | 467 | 0.82 | 0.84 | 0.83 | 0.86 | 0.00 | 0.87 | 0.81 | 0.86 | 0.85 |
| CD4-positive, alpha-beta T cell | 419 | 0.84 | 0.88 | 0.87 | 0.61 | 0.00 | 0.80 | 0.84 | 0.87 | 0.90 |
| plasma cell | 315 | 0.98 | 0.98 | 0.97 | 0.99 | 0.00 | 0.99 | 0.99 | 0.98 | 0.99 |
| CD8-positive, alpha-beta T cell | 295 | 0.80 | 0.85 | 0.83 | 0.45 | 0.00 | 0.77 | 0.82 | 0.85 | 0.88 |
| fibroblast | 236 | 0.97 | 0.97 | 0.97 | 0.96 | 0.00 | 0.96 | 0.97 | 0.97 | 0.97 |
| B cell | 225 | 0.99 | 0.98 | 0.98 | 0.98 | 0.00 | 0.99 | 0.99 | 0.98 | 0.99 |
| gamma-delta T cell | 190 | 0.88 | 0.90 | 0.92 | 0.55 | 0.00 | 0.83 | 0.88 | 0.90 | 0.92 |
| intestinal crypt stem cell of colon | 152 | 0.82 | 0.86 | 0.83 | 0.79 | 0.00 | 0.78 | 0.82 | 0.82 | 0.88 |
| large intestine goblet cell | 180 | 0.92 | 0.95 | 0.94 | 0.63 | 0.00 | 0.70 | 0.92 | 0.88 | 0.92 |
| enteroglial cell | 76 | 0.99 | 0.97 | 0.97 | 0.99 | 0.00 | 0.97 | 0.99 | 0.99 | 0.99 |
| regulatory T cell | 59 | 0.63 | 0.66 | 0.67 | 0.38 | 0.00 | 0.51 | 0.71 | 0.74 | 0.77 |
| mast cell | 46 | 0.99 | 0.98 | 0.98 | 0.96 | 0.00 | 0.99 | 0.99 | 0.99 | 0.99 |
| myeloid leukocyte | 33 | 0.83 | 0.93 | 0.91 | 0.85 | 0.00 | 0.93 | 0.93 | 0.96 | 0.98 |
| colon endothelial cell | 38 | 0.93 | 0.90 | 0.92 | 0.89 | 0.00 | 0.90 | 0.95 | 0.96 | 0.95 |
| colon macrophage | 36 | 0.79 | 0.86 | 0.79 | 0.83 | 0.00 | 0.89 | 0.89 | 0.95 | 0.94 |
| transit amplifying cell of colon | 26 | 0.72 | 0.60 | 0.65 | 0.49 | 0.00 | 0.51 | 0.62 | 0.71 | 0.73 |
| naive thymus-derived CD4-positive, alpha-beta T cell | 22 | 0.82 | 0.84 | 0.71 | 0.24 | 0.00 | 0.79 | 0.74 | 0.80 | 0.84 |
| paneth cell of colon | 20 | 0.56 | 0.36 | 0.58 | 0.70 | 0.00 | 0.62 | 0.71 | 0.51 | 0.75 |
| tuft cell of colon | 18 | 0.97 | 1.00 | 1.00 | 0.88 | 0.00 | 0.82 | 0.97 | 0.92 | 0.97 |
| myofibroblast cell | 8 | 0.73 | 0.84 | 0.89 | 0.86 | 0.00 | 0.94 | 1.00 | 0.84 | 0.84 |
| monocyte | 4 | 0.86 | 0.86 | 0.40 | 0.67 | 0.00 | 0.86 | 0.86 | 0.86 | 0.86 |
| mature NK T cell | 6 | 0.00 | 0.25 | 0.00 | 0.29 | 0.00 | 0.18 | 0.55 | 0.60 | 0.25 |
| type L enteroendocrine cell | 3 | 0.44 | 0.67 | 0.57 | 0.80 | 0.00 | 0.44 | 0.80 | 0.40 | 0.80 |
| myeloid dendritic cell | 3 | 0.00 | 0.00 | 0.00 | 0.00 | 0.00 | 0.00 | 0.00 | 0.50 | 0.00 |
| enterochromaffin-like cell | 3 | 0.00 | 1.00 | 0.00 | 0.50 | 0.00 | 0.67 | 0.80 | 0.86 | 0.80 |
| natural killer cell | 1 | 0.00 | 0.00 | 0.00 | 0.00 | 0.00 | 0.18 | 0.00 | 0.00 | 0.00 |
| innate lymphoid cell | 2 | 0.00 | 0.00 | 0.00 | 0.00 | 0.00 | 0.25 | 0.00 | 0.00 | 0.00 |
| neutrophil | 0 | 0.00 | 0.00 | 0.00 | 0.00 | 0.00 | 0.00 | 0.00 | 0.00 | 0.00 |
The train accuracies are computed on the training data.
| Cell Type | N cells | celltypist | knn bbknn | knn harmony | knn on scvi | onclass | scanvi | svm | xgboost | Consensus Prediction |
| --- | --- | --- | --- | --- | --- | --- | --- | --- | --- | --- |
| enterocyte of epithelium of large intestine | 4100 | 0.84 | 0.85 | 0.87 | 0.91 | 0.00 | 0.89 | 0.85 | 0.89 | 0.88 |
| CD4-positive, alpha-beta T cell | 3821 | 0.85 | 0.88 | 0.91 | 0.77 | 0.00 | 0.86 | 0.87 | 0.90 | 0.92 |
| plasma cell | 2993 | 0.98 | 0.98 | 0.99 | 0.99 | 0.00 | 0.98 | 0.99 | 0.98 | 0.99 |
| CD8-positive, alpha-beta T cell | 2667 | 0.79 | 0.84 | 0.88 | 0.63 | 0.00 | 0.81 | 0.85 | 0.88 | 0.89 |
| fibroblast | 2361 | 0.98 | 0.98 | 0.98 | 0.98 | 0.00 | 0.98 | 0.99 | 0.99 | 0.99 |
| B cell | 1844 | 0.99 | 0.99 | 0.99 | 0.98 | 0.00 | 0.98 | 0.99 | 0.99 | 0.99 |
| gamma-delta T cell | 1666 | 0.91 | 0.92 | 0.95 | 0.68 | 0.00 | 0.89 | 0.92 | 0.93 | 0.96 |
| intestinal crypt stem cell of colon | 1580 | 0.84 | 0.86 | 0.91 | 0.87 | 0.00 | 0.85 | 0.90 | 0.87 | 0.92 |
| large intestine goblet cell | 1377 | 0.92 | 0.95 | 0.94 | 0.67 | 0.00 | 0.70 | 0.94 | 0.94 | 0.95 |
| enteroglial cell | 772 | 0.99 | 0.98 | 0.99 | 0.99 | 0.00 | 0.99 | 0.99 | 0.99 | 0.99 |
| regulatory T cell | 574 | 0.73 | 0.71 | 0.81 | 0.45 | 0.00 | 0.70 | 0.80 | 0.81 | 0.87 |
| mast cell | 347 | 0.98 | 0.97 | 0.98 | 0.95 | 0.00 | 0.98 | 0.99 | 0.99 | 0.99 |
| myeloid leukocyte | 321 | 0.85 | 0.90 | 0.92 | 0.87 | 0.00 | 0.94 | 0.99 | 1.00 | 0.96 |
| colon endothelial cell | 310 | 0.90 | 0.88 | 0.93 | 0.91 | 0.00 | 0.93 | 0.99 | 0.96 | 0.94 |
| colon macrophage | 292 | 0.79 | 0.85 | 0.86 | 0.81 | 0.00 | 0.95 | 0.99 | 1.00 | 0.94 |
| transit amplifying cell of colon | 211 | 0.75 | 0.69 | 0.81 | 0.65 | 0.00 | 0.52 | 0.80 | 0.80 | 0.87 |
| naive thymus-derived CD4-positive, alpha-beta T cell | 206 | 0.68 | 0.64 | 0.76 | 0.42 | 0.00 | 0.75 | 0.83 | 0.82 | 0.84 |
| paneth cell of colon | 197 | 0.63 | 0.35 | 0.81 | 0.78 | 0.00 | 0.79 | 0.85 | 0.58 | 0.89 |
| tuft cell of colon | 160 | 0.93 | 0.96 | 0.95 | 0.92 | 0.00 | 0.85 | 0.98 | 0.96 | 0.97 |
| myofibroblast cell | 74 | 0.82 | 0.83 | 0.88 | 0.87 | 0.00 | 0.93 | 1.00 | 0.98 | 0.95 |
| monocyte | 62 | 0.78 | 0.80 | 0.79 | 0.74 | 0.00 | 0.97 | 0.99 | 0.98 | 0.93 |
| mature NK T cell | 41 | 0.00 | 0.49 | 0.69 | 0.48 | 0.00 | 0.69 | 0.95 | 0.96 | 0.91 |
| type L enteroendocrine cell | 41 | 0.77 | 0.88 | 0.94 | 0.71 | 0.00 | 0.79 | 0.99 | 0.92 | 1.00 |
| myeloid dendritic cell | 37 | 0.00 | 0.14 | 0.45 | 0.19 | 0.00 | 0.90 | 1.00 | 1.00 | 0.75 |
| enterochromaffin-like cell | 24 | 0.00 | 0.79 | 0.88 | 0.82 | 0.00 | 0.63 | 1.00 | 0.94 | 0.96 |
| natural killer cell | 13 | 0.00 | 0.00 | 0.13 | 0.14 | 0.00 | 0.36 | 1.00 | 0.96 | 0.92 |
| innate lymphoid cell | 11 | 0.00 | 0.00 | 0.53 | 0.15 | 0.00 | 0.61 | 0.96 | 1.00 | 0.78 |
| neutrophil | 9 | 0.00 | 0.00 | 0.36 | 0.71 | 0.00 | 0.90 | 1.00 | 0.95 | 1.00 |
</details>
# References
Tabula Sapiens reveals transcription factor expression, senescence effects, and sex-specific features in cell types from 28 human organs and tissues, The Tabula Sapiens Consortium; bioRxiv, doi: https://doi.org/10.1101/2024.12.03.626516
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