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App.py
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import streamlit as st
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import zipfile
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import urllib.request
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import glob
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import SigProfilerMatrixGenerator
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from SigProfilerMatrixGenerator import install as genInstall
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import shutil
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import os
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from SigProfilerExtractor import sigpro as sig
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import sys
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import base64
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import streamlit.components.v1 as components
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curdir= os.getcwd()
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def remove_old_vcf():
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vcfrem=glob.glob('input/*.vcf')
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for filepath in vcfrem:
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os.remove(filepath)
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vcfrem=glob.glob('input/input/*.vcf')
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for filepath in vcfrem:
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os.remove(filepath)
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def show_pdf(file_path):
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with open(file_path,"rb") as f:
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base64_pdf = base64.b64encode(f.read()).decode('utf-8')
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pdf_display = f'<iframe src="data:application/pdf;base64,{base64_pdf}" width="1500" height="1000" type="application/pdf"></iframe>'
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st.markdown(pdf_display, unsafe_allow_html=True)
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if st.button('get reference genome'):
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st.write(os.path.dirname(SigProfilerMatrixGenerator.__file__))
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dirtest=os.path.dirname(SigProfilerMatrixGenerator.__file__)
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#st.write(sys.path)
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urllib.request.urlretrieve('https://dl.dropboxusercontent.com/s/et97ewsct862x7m/references.zip?dl=0','references.zip')
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with zipfile.ZipFile('references.zip', 'r') as zip_ref:
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zip_ref.extractall(dirtest)
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seev=glob.glob('/home/appuser/venv/lib/python3.9/site-packages/SigProfilerMatrixGenerator/references/*')
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for i in seev:
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st.write(i)
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##genInstall.install('GRCh37')
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if not os.path.exists('input'):
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os.mkdir('input')
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if not os.path.exists('input/input'):
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os.mkdir('input/input')
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file_to_lookat=st.file_uploader('VCF upload here',type=[".vcf"],accept_multiple_files=True)
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remove_old_vcf()
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if file_to_lookat !=[]:
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bytes_data=file_to_lookat[0].read()
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with open(os.path.join("input",file_to_lookat[0].name),"wb") as f:
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f.write(bytes_data)
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f.close()
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#vcfuse=glob.glob('file_to_lookat[0].name')[0]
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#shutil.copy2(vcfuse,'input/'+vcfuse)
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#pdb.set_trace()
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with st.spinner('computing signatures'):
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sig.sigProfilerExtractor("vcf", "output", "input", minimum_signatures=1, maximum_signatures=3)
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show_pdf('output/SBS96/Suggested_Solution/COSMIC_SBS96_Decomposed_Solution/SBS96_Decomposition_Plots.pdf')
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components.iframe("https://cancer.sanger.ac.uk/signatures/sbs/", height=3000,width=800)
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show_pdf('output/ID83/Suggested_Solution/COSMIC_ID83_Decomposed_Solution/ID83_Decomposition_Plots.pdf')
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components.iframe("https://cancer.sanger.ac.uk/signatures/id/",height=3000,width=800)
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remove_old_vcf()
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