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import gradio as gr
from igfold import IgFoldRunner
import os
import random
import base64


def read_mol(molpath):
    with open(molpath, "r") as fp:
        lines = fp.readlines()
    mol = ""
    for l in lines:
        mol += l
    return mol


def molecule(input_pdb, h_seq, l_seq):

    mol = read_mol(input_pdb)

    byte_content = mol.encode('utf-8')
    base64_content = base64.b64encode(byte_content).decode('utf-8')


    x = (
        """<!DOCTYPE html>
        <html>
        <head>    
    <meta http-equiv="content-type" content="text/html; charset=UTF-8" />
    <style>
    body{
        font-family:sans-serif
    }
    .mol-container {
    width: 100%;
    height: 600px;
    position: relative;
    }
    .mol-container select{
        background-image:None;
    }
    </style>
     <script src="https://cdnjs.cloudflare.com/ajax/libs/jquery/3.6.3/jquery.min.js" integrity="sha512-STof4xm1wgkfm7heWqFJVn58Hm3EtS31XFaagaa8VMReCXAkQnJZ+jEy8PCC/iT18dFy95WcExNHFTqLyp72eQ==" crossorigin="anonymous" referrerpolicy="no-referrer"></script>
     <script src="https://3Dmol.csb.pitt.edu/build/3Dmol-min.js"></script>
    </head>
    <body>  
    <div id="container" class="mol-container"></div>
  
        <script>

        let h_seq = `"""
        + h_seq
        + """`

        let l_seq = `"""
        + l_seq
        + """`

        let pdb = `"""
        + mol
        + """`  
             $(document).ready(function () {
                let element = $("#container");
                let config = { backgroundColor: "white" };
                let viewer = $3Dmol.createViewer(element, config);
                viewer.addModel(pdb, "pdb");
                viewer.getModel(0).setStyle({chain: "H"}, {cartoon:{color:"red"}});
                viewer.getModel(0).setStyle({chain: "L"}, {cartoon:{color:"blue"}});
                viewer.addSurface($3Dmol.SurfaceType.VDW, {opacity: 0.4, color: "lightblue"});
                viewer.addResLabels(h_seq, {chain: "H"});
                viewer.addResLabels(l_seq, {chain: "L"});
                viewer.zoomTo();
                viewer.render();
                viewer.zoom(0.8, 2000);
              })
        </script>
        </body></html>"""
    )

    return f"""<iframe style="width: 100%; height: 600px" name="result" allow="midi; geolocation; microphone; camera; 
    display-capture; encrypted-media;" sandbox="allow-modals allow-forms 
    allow-scripts allow-same-origin allow-popups 
    allow-top-navigation-by-user-activation allow-downloads" allowfullscreen="" 
    allowpaymentrequest="" frameborder="0" srcdoc='{x}'></iframe>
  <div style="position: absolute; top: 10px; right: 10px; background-color: white; padding: 10px; border: 1px solid black;">
        <div style="width: 20px; height: 20px; background-color: red; display: inline-block;"></div>
        <span style="margin-left: 5px;">Heavy chain</span><br>
        <div style="width: 20px; height: 20px; background-color: blue; display: inline-block;"></div>
        <span style="margin-left: 5px;">Light chain</span>
        <div style="display: flex; justify-content: space-between; margin-top: 5px;">
        <a href="data:application/octet-stream;base64,{base64_content}" download="structure.pdb">Download File</a>
        </div>
    </div>
    """

def validate(seq):
    alphabet = set('ACDEFGHIKLMNPQRSTVWY')
    leftover = set(seq.upper()) - alphabet
    return not leftover

def pred_seq(h_seq, l_seq):
    h_seq = h_seq.upper().replace(' ', '')
    l_seq = l_seq.upper().replace(' ', '')
    print(h_seq)
    print(l_seq)
    h_is_valid = validate(h_seq)
    l_is_valid = validate(l_seq)
    if h_is_valid and l_is_valid:    
        sequences = {
            "H": h_seq,
            "L": l_seq
        }

        f_name = ''.join([random.choice("ACDEFGHIKLMNPQRSTVWY") for _ in range(15)])

        pred_pdb = f"{f_name}.pdb"

        igfold = IgFoldRunner(num_models = 1)
        igfold.fold(
            pred_pdb,
            sequences=sequences,
            do_refine=False,
            do_renum=False
        )

        html = molecule(pred_pdb, h_seq, l_seq)

    else:
        html = "<p>ERROR! Not valid sequence</p>"
    return (html)

with gr.Blocks() as demo:
    gr.Markdown('# Antibody Structure Prediction')
    gr.Markdown("## Examples")
    html = """
    <p>
       <strong>H-chain:</strong> <tt>QVQLKESGPGLVAPSQSLSITCTVSGFSLSSYGVSWVRQPPGKGLEWLGVIWGDGSTNYHPNLMSRLSISKDISKSQVLFKLNSLQTDDTATYYCVTLDYWGQGTSVTVSS</tt>
       <br/>
       <strong>L-chain: </strong> <tt>DVVMTQTPLSLPVSLGDQASISCRSSQSLVHRNGNTYLHWYLQKPGQSPKLLIYKVSNRFSGVPDRFSGSGSGTDFTLKISRVEAEDLGLYFCFQTTYVPNTFGGGTKLEIK</tt>
    </p>

    <p>
       <strong>H-chain:</strong> <tt>EVQLLESGGGLVQPGGSLRLSCAASGFTFSLYWMGWVRQAPGKGLEWVSSISSSGGVTPYADSVKGRFTISRDNSKNTLYLQMNSLRAEDTAVYYCAKLGELGWFDPWGQGTLVTVSS</tt>
       <br/>
       <strong>L-chain: </strong> <tt>DIQMTQSPSSLSASVGDRVTITCRASQGISSYLNWYQQKPGKAPKLLIYYASNLQNGVPSRFSGSGSGTDFTLTISSLQPEDFATYYCQQSYSTPLTFGGGTKVEIK</tt>
    </p>

    <p>
       <strong>H-chain:</strong> <tt>EVQLVQSGPEVKKPGTSVKVSCKASGFTFMSSAVQWVRQARGQRLEWIGWIVIGSGNTNYAQKFQERVTITRDMSTSTAYMELSSLRSEDTAVYYCAAPYCSSISCNDGFDIWGQGTMVTVS</tt>
       <br/>
       <strong>L-chain: </strong> <tt>EIVLTQSPATLSLSPGERATLSCRASQSVSSYLAWYQQKPGQAPRLLIYDASNRATGIPARFSGSGSGTDFTLTISSLEPEDFAVYYCQQRSNWPITFGQGTKLEIK</tt>
    </p>
    """
    gr.HTML(value = html)
    h_text = gr.Textbox(lines=5, label="Heavy chain")
    l_text = gr.Textbox(lines=5, label="Light chain")
    btn = gr.Button(value="Submit")
    btn.click(pred_seq, inputs=[h_text, l_text], outputs=gr.HTML())
if __name__ == "__main__":
    demo.launch()