id
stringlengths 7
10
| title
stringlengths 8
354
| content
stringlengths 91
4.1k
| contents
stringlengths 164
4.24k
| embedding
sequencelengths 384
384
|
|---|---|---|---|---|
PMID:25008 | Effect of 3-[bis-(2-hydroxyethyl)-amino]-acetophenone-[4.5-diphenyl-oxazolyl-(2)]-hydrazone (IMET 98/69) on lethal mengo virus encephalitis. | 3-[Bis-(2-hydroxyethyl)-amino]-acetophenone-[4.5-diphenyl-oxazolyl-(2)]-hydrazone (IMET 98/69) was found to afford maximum "rates of protection" in intraperitoneally (i.p.) or intranasally (i.n.) induced Mengo virus encephalitis in mice when administered once daily at doses of 1 mmole/kg (456.5 mg/kg) subcutaneously (s.c.) or 4 mmoles/kg (1 826 mg/kg) perorally (p.o.). Three days of treatment were sufficient if started on the day before or at the time of virus inoculation. Initiation of treatment 6 hours after inoculation was no longer effective. In a remarkable number of brains from infected and treated mice no virus was detectable, while in the remaining brains the appearance of virus was strongly delayed, and its amount significantly reduced. | Effect of 3-[bis-(2-hydroxyethyl)-amino]-acetophenone-[4.5-diphenyl-oxazolyl-(2)]-hydrazone (IMET 98/69) on lethal mengo virus encephalitis. 3-[Bis-(2-hydroxyethyl)-amino]-acetophenone-[4.5-diphenyl-oxazolyl-(2)]-hydrazone (IMET 98/69) was found to afford maximum "rates of protection" in intraperitoneally (i.p.) or intranasally (i.n.) induced Mengo virus encephalitis in mice when administered once daily at doses of 1 mmole/kg (456.5 mg/kg) subcutaneously (s.c.) or 4 mmoles/kg (1 826 mg/kg) perorally (p.o.). Three days of treatment were sufficient if started on the day before or at the time of virus inoculation. Initiation of treatment 6 hours after inoculation was no longer effective. In a remarkable number of brains from infected and treated mice no virus was detectable, while in the remaining brains the appearance of virus was strongly delayed, and its amount significantly reduced. | [
-0.04953311011195183,
-0.008160306140780449,
-0.02023807168006897,
-0.023009514436125755,
0.07395439594984055,
-0.015464103780686855,
0.038871243596076965,
0.11519574373960495,
-0.025936612859368324,
-0.002029295777902007,
-0.0029840441420674324,
-0.026342928409576416,
0.00472085177898407,
0.04523750767111778,
0.0070392363704741,
0.02065141685307026,
0.013801601715385914,
-0.05142049118876457,
-0.05934121087193489,
0.05454321578145027,
-0.059019822627305984,
-0.006613283418118954,
-0.010810230858623981,
-0.058487921953201294,
0.03583816811442375,
-0.04134444519877434,
0.030021965503692627,
0.028632014989852905,
-0.029750995337963104,
-0.1993224024772644,
-0.006688207853585482,
-0.014461498707532883,
-0.04178357496857643,
-0.054756056517362595,
-0.0653340220451355,
0.04215984418988228,
-0.023121314123272896,
-0.00011384916433598846,
-0.012890498153865337,
-0.03493083640933037,
0.08057942986488342,
0.08338487893342972,
-0.01596810296177864,
-0.014533789828419685,
-0.047966670244932175,
-0.03764839470386505,
-0.031198708340525627,
-0.028310323134064674,
0.06900964677333832,
-0.006083749700337648,
0.0018035767134279013,
-0.01863839663565159,
-0.010739232413470745,
0.03353732079267502,
0.020715776830911636,
0.009769991040229797,
-0.05284732207655907,
0.02688138373196125,
-0.0686863586306572,
0.04551001638174057,
0.02044752612709999,
0.025063956156373024,
-0.1794130653142929,
0.08535847067832947,
-0.01473385002464056,
-0.015114761888980865,
-0.027586471289396286,
-0.06549052894115448,
0.03865235298871994,
0.10741779208183289,
-0.05418429151177406,
-0.0006331104086712003,
-0.00013032363494858146,
-0.0020074783824384212,
0.04395348206162453,
-0.00037070640246383846,
0.025083554908633232,
-0.06801936030387878,
0.018173884600400925,
-0.006856891326606274,
-0.029084622859954834,
-0.009232097305357456,
0.024658434092998505,
-0.022831398993730545,
0.043944403529167175,
0.01586983911693096,
0.046579837799072266,
0.01456911489367485,
0.006498578004539013,
0.007107825949788094,
0.008950433693826199,
0.028557423502206802,
-0.026720911264419556,
-0.00745039526373148,
0.011299551464617252,
0.01331246830523014,
0.04279230535030365,
-0.03795899450778961,
-0.024362953379750252,
0.333335280418396,
-0.001567958272062242,
0.03710799664258957,
-0.09141603857278824,
-0.034435562789440155,
-0.007382787764072418,
0.021649911999702454,
0.003913313616067171,
-0.04157025367021561,
-0.007939399220049381,
0.04087644815444946,
-0.02742549031972885,
0.03394921496510506,
0.05653085559606552,
0.03189030662178993,
-0.05060829222202301,
0.03659377619624138,
0.04019767791032791,
0.02601146697998047,
0.009185236878693104,
-0.0010840743780136108,
0.0909964069724083,
-0.045170772820711136,
0.051402151584625244,
0.008566493168473244,
0.03805667906999588,
0.05205848813056946,
-0.011503993533551693,
0.0887303575873375,
-0.003773684147745371,
-0.0008784966776147485,
-0.008782321587204933,
-0.02472717873752117,
-0.031671129167079926,
0.055372703820466995,
-0.008373375050723553,
0.007947505451738834,
-0.03334780037403107,
-0.010062485001981258,
0.00020619718998204917,
-0.07470832765102386,
-0.004558915738016367,
-0.018324967473745346,
0.007884802296757698,
-0.11249475926160812,
-0.0972038209438324,
0.06718600541353226,
-0.024636222049593925,
-0.01826552487909794,
0.007513869553804398,
0.004593252670019865,
0.0558478944003582,
0.10187865793704987,
-0.04837222397327423,
0.04254486411809921,
-0.023834576830267906,
-0.001678873086348176,
0.010248290374875069,
0.028876934200525284,
-0.02689916267991066,
0.006180271040648222,
-0.13572977483272552,
-0.04999277740716934,
0.0032239072024822235,
0.10513381659984589,
-0.02391486056149006,
-0.036643728613853455,
-0.0127578005194664,
-0.01628417707979679,
0.04482000693678856,
-0.005617154762148857,
-0.007008126936852932,
-0.026264604181051254,
0.059684641659259796,
-0.01829899474978447,
-0.009718846529722214,
-0.043462712317705154,
0.011231724172830582,
0.018622778356075287,
0.011678493581712246,
0.04215690493583679,
-0.0012902208836749196,
0.006248027551919222,
-0.008739315904676914,
0.06098242476582527,
-0.008364617824554443,
-0.012915967963635921,
-0.020105259492993355,
0.03649511560797691,
0.06237117201089859,
-0.03989056497812271,
-0.03796807676553726,
0.0211427491158247,
-0.03251037001609802,
-0.010774918831884861,
0.025361455976963043,
-0.042373646050691605,
-0.07032931596040726,
0.04988960176706314,
-0.0011692007537931204,
-0.055699363350868225,
0.10024573653936386,
-0.02280784212052822,
0.03506195917725563,
-0.0074212998151779175,
0.034954093396663666,
0.11868198961019516,
0.03346138074994087,
-0.08526720851659775,
0.028037549927830696,
-0.045738015323877335,
-0.03363249823451042,
-0.025692777708172798,
-0.01859966665506363,
-0.0024983910843729973,
-0.0011533118085935712,
0.09936677664518356,
-0.03906918689608574,
0.040899790823459625,
0.016068877652287483,
0.03867741674184799,
0.05276019498705864,
-0.010939941741526127,
-0.06078895926475525,
-0.2876916229724884,
-0.02906777337193489,
-0.016849316656589508,
-0.028722818940877914,
0.03727799654006958,
-0.005867409519851208,
0.01861400716006756,
-0.08266395330429077,
-0.0014634947292506695,
-0.027165252715349197,
-0.005271521862596273,
0.08077950030565262,
0.0039656502194702625,
-0.004678158089518547,
-0.04569422826170921,
0.04270188882946968,
0.011539601720869541,
-0.040070515125989914,
0.04068182408809662,
-0.003478721948340535,
0.020905056968331337,
-0.03130778670310974,
-0.05140189081430435,
0.02643721178174019,
0.0352388396859169,
-0.02858002670109272,
0.14326360821723938,
0.02449995093047619,
0.014558007009327412,
-0.014204971492290497,
-0.00867618340998888,
0.05588378384709358,
0.057489193975925446,
-0.04609121382236481,
-0.023313794285058975,
-0.05573355406522751,
-0.003559054806828499,
-0.02369396574795246,
-0.013730611652135849,
-0.006127903237938881,
-0.033660586923360825,
-0.03293750435113907,
0.024765856564044952,
-0.058480847626924515,
0.012381283566355705,
0.01421184092760086,
0.00048657908337190747,
0.030072730034589767,
-0.010365311987698078,
-0.02158987708389759,
0.020125536248087883,
0.014995560981333256,
-0.010889511555433273,
0.02616761438548565,
0.029372310265898705,
-0.035132598131895065,
-0.052417874336242676,
0.012367406859993935,
-0.03923818841576576,
-0.007424998562783003,
0.020529113709926605,
-0.0031831932719796896,
0.02193565107882023,
-0.05511423572897911,
-0.009218567050993443,
-0.04375530034303665,
0.03694918006658554,
0.012207292951643467,
0.10073201358318329,
0.07371977716684341,
0.00022092180734034628,
0.11068284511566162,
-0.02157074771821499,
-0.0015271930024027824,
0.052633363753557205,
-0.0013276422396302223,
0.040388111025094986,
-0.0180484801530838,
-0.09075506776571274,
0.024036122485995293,
-0.06052319332957268,
-0.004956278949975967,
0.0007997354841791093,
-0.05256879702210426,
0.014388049021363258,
0.04509907215833664,
-0.015253360383212566,
-0.029789136722683907,
0.05235520750284195,
0.033243853598833084,
0.019089331850409508,
-0.045666974037885666,
-0.02389542944729328,
0.009981228038668633,
0.008678005076944828,
-0.024419786408543587,
-0.2820459306240082,
0.003882720833644271,
-0.009015507996082306,
-0.016987500712275505,
0.005638387519866228,
0.004795193672180176,
0.005016863811761141,
0.0044808657839894295,
-0.07241075485944748,
-0.057044923305511475,
0.017624681815505028,
0.042948924005031586,
0.054161034524440765,
0.058915507048368454,
-0.0008957340614870191,
0.030902350321412086,
0.026743801310658455,
0.006282289046794176,
0.013006092049181461,
0.008503534831106663,
-0.03419794887304306,
-0.06823264062404633,
0.15935787558555603,
0.01015554554760456,
-0.006150856148451567,
0.06630253046751022,
-0.02090686745941639,
0.09370049089193344,
-0.03601584956049919,
-0.0023282624315470457,
-0.018721723929047585,
-0.021496843546628952,
0.06511522084474564,
0.00005308412073645741,
0.044191185384988785,
0.018191585317254066,
-0.04776536673307419,
0.041563957929611206,
0.008364023640751839,
0.015891551971435547,
-0.03577466681599617,
0.0000631866860203445,
0.014234066009521484,
0.04456965625286102,
0.032241083681583405,
-0.012482704594731331,
-0.009880655445158482,
-0.07812854647636414,
-0.04359859973192215,
0.02804967574775219,
-0.008940081112086773,
-0.040316142141819,
0.02635977789759636,
-0.04714973270893097,
0.016527796164155006,
-0.0029754256829619408,
0.008932194672524929,
0.058644849807024,
-0.010496247559785843,
0.0191220510751009,
0.04991724714636803,
-0.02949797362089157,
-0.003413998754695058,
0.07316861301660538,
-0.01583636924624443
] |
PMID:25009 | Reproducible plaquing system for rhinovirus serotypes in HeLa cells--agarose suspension. | HeLa cell--agarose suspension method provides an excellent plaquing system for assay of rhinovirus serotypes which ordinarily produce indistinct plaques on cell monolayers. The variable plaque size of cloned virus progeny does not involve antigenic change in phenotype as shown by plaque neutralization test. The system has proved useful for accurate and reproducible plaque assay of rhinoviruses, assay of serum neutralizing antibodies and cloning of viruses. The plaques produced are large, clear and reproducible. The factors which influence the plaquing of rhinoviruses in this system are the age of the cells, cell density and pH of the medium. | Reproducible plaquing system for rhinovirus serotypes in HeLa cells--agarose suspension. HeLa cell--agarose suspension method provides an excellent plaquing system for assay of rhinovirus serotypes which ordinarily produce indistinct plaques on cell monolayers. The variable plaque size of cloned virus progeny does not involve antigenic change in phenotype as shown by plaque neutralization test. The system has proved useful for accurate and reproducible plaque assay of rhinoviruses, assay of serum neutralizing antibodies and cloning of viruses. The plaques produced are large, clear and reproducible. The factors which influence the plaquing of rhinoviruses in this system are the age of the cells, cell density and pH of the medium. | [
-0.004006457980722189,
0.016482116654515266,
0.014831416308879852,
-0.022079449146986008,
-0.01872282847762108,
0.01860087923705578,
0.08490767329931259,
0.009900879114866257,
0.008090204559266567,
-0.0104928407818079,
-0.013529939576983452,
-0.09483692795038223,
0.06447675824165344,
0.013082195073366165,
-0.04158109426498413,
-0.02521435171365738,
-0.002818057779222727,
0.04296063631772995,
0.0029603319708257914,
-0.00014833502063993365,
-0.025378229096531868,
-0.06669364869594574,
-0.03701433166861534,
-0.03653948754072189,
0.07789318263530731,
0.017741883173584938,
-0.041251663118600845,
0.010501740500330925,
-0.026471851393580437,
-0.20312608778476715,
0.02038474939763546,
-0.11110235005617142,
-0.02157513238489628,
-0.026694072410464287,
-0.0286012664437294,
-0.0072333249263465405,
-0.013990988954901695,
-0.05098291486501694,
-0.007009353023022413,
0.051041703671216965,
0.02964424528181553,
0.013889500871300697,
-0.04010056331753731,
0.010146735236048698,
-0.0038421337958425283,
-0.025495652109384537,
-0.05571369826793671,
0.01781642436981201,
0.03509730473160744,
-0.01651761494576931,
0.0008552405051887035,
-0.05180824548006058,
-0.024098655208945274,
0.014751461334526539,
-0.06508273631334305,
0.003674800042062998,
-0.037406668066978455,
0.05508360266685486,
0.019818738102912903,
-0.0287938229739666,
0.019215239211916924,
-0.009730855002999306,
-0.14314593374729156,
0.0735868439078331,
0.0494009368121624,
-0.0044218916445970535,
0.02033356949687004,
-0.06831658631563187,
0.0008409183355979621,
0.06506724655628204,
-0.08875814080238342,
0.036207061260938644,
-0.031035589054226875,
0.04800790548324585,
-0.0038657996337860823,
0.010377570055425167,
-0.017986059188842773,
-0.030962815508246422,
0.01462585385888815,
0.03476255387067795,
-0.060214027762413025,
-0.010148081928491592,
0.04362811520695686,
0.013013540767133236,
0.007185418624430895,
0.06313435733318329,
-0.038164302706718445,
-0.035167209804058075,
0.03580085560679436,
-0.0052916486747562885,
0.014397201128304005,
0.023387905210256577,
0.004648195579648018,
0.004170681349933147,
-0.07581670582294464,
0.00015033676754683256,
0.0015325261047109962,
-0.028465401381254196,
0.07649123668670654,
0.37093979120254517,
0.021148789674043655,
-0.013519401662051678,
0.0044625299051404,
-0.011524474248290062,
0.00391684053465724,
-0.019414611160755157,
-0.01787205971777439,
-0.0245515163987875,
-0.05015001446008682,
0.07436159998178482,
0.048281192779541016,
0.02121354453265667,
0.030365169048309326,
-0.07709048688411713,
0.02672661654651165,
-0.01957636885344982,
0.0184136014431715,
0.008340430445969105,
-0.07969940453767776,
0.03621778264641762,
-0.01145541574805975,
-0.07568711787462234,
-0.008257582783699036,
-0.04932064190506935,
0.11769941449165344,
0.03558231517672539,
0.031361956149339676,
0.13890445232391357,
0.05284564197063446,
0.012867751531302929,
0.0007124614203348756,
-0.017247026786208153,
0.002150059910491109,
-0.007700162474066019,
0.006840285379439592,
0.002641885308548808,
0.052338432520627975,
-0.025247661396861076,
0.010326318442821503,
0.016584321856498718,
-0.0004316289850976318,
-0.02680654637515545,
-0.0018196309683844447,
-0.07714547216892242,
0.004728294443339109,
0.06852976232767105,
-0.05345844104886055,
0.06904041022062302,
0.008679154329001904,
-0.08591747283935547,
-0.008208994753658772,
0.05101916193962097,
-0.017705850303173065,
0.01180045586079359,
0.023812977597117424,
0.003132474608719349,
-0.029216336086392403,
0.02057248167693615,
0.004084021784365177,
0.05042927339673042,
-0.030710801482200623,
-0.03618801385164261,
0.014553485438227654,
0.048537593334913254,
0.014564630575478077,
-0.07915959507226944,
0.009127378463745117,
0.016889382153749466,
0.08554759621620178,
0.0338270328938961,
-0.0239250548183918,
-0.030233193188905716,
-0.036295466125011444,
0.023856351152062416,
-0.042690157890319824,
0.011853024363517761,
-0.037743113934993744,
0.010585613548755646,
0.02558804675936699,
0.006316557992249727,
-0.0065613375045359135,
-0.03414369747042656,
0.0013136224588379264,
0.007859509438276291,
0.026082230731844902,
-0.018286436796188354,
-0.06291907280683517,
0.02244093455374241,
0.054484982043504715,
-0.011037416756153107,
-0.008901008404791355,
0.01778699830174446,
-0.025788437575101852,
0.02024232968688011,
0.017190491780638695,
-0.0036163735203444958,
0.00478752376511693,
0.013979868032038212,
0.007875180803239346,
0.011691058985888958,
0.08188128471374512,
0.02339787408709526,
0.01497110165655613,
-0.013956262730062008,
0.04641031473875046,
0.0676710307598114,
0.03655960038304329,
-0.029764974489808083,
0.050739679485559464,
0.02743344008922577,
-0.03360547870397568,
0.005908828694373369,
-0.052373453974723816,
-0.0063071358017623425,
-0.08330965787172318,
0.07665444165468216,
-0.03437680006027222,
-0.06147373467683792,
0.02327871322631836,
-0.005140680354088545,
0.043856676667928696,
0.03301384299993515,
0.0446576252579689,
-0.2977622449398041,
-0.0189158134162426,
0.030092380940914154,
-0.011127936653792858,
0.025784721598029137,
-0.08564725518226624,
-0.008367124013602734,
-0.05715353786945343,
0.04619922116398811,
-0.05050143599510193,
0.06996206939220428,
0.07773277163505554,
0.0049365549348294735,
-0.01438282523304224,
-0.012609162367880344,
0.07378047704696655,
-0.04580749198794365,
0.004512161482125521,
0.0029855817556381226,
0.01618182845413685,
-0.012879284098744392,
-0.01235920935869217,
0.03481690213084221,
0.020760975778102875,
0.06700413674116135,
0.032762352377176285,
0.12613485753536224,
-0.01052449457347393,
0.03324984014034271,
-0.002786272205412388,
-0.0020012359600514174,
0.055702634155750275,
0.02620348148047924,
-0.02565642260015011,
-0.0026068957522511482,
0.03966474160552025,
-0.053903717547655106,
-0.007019558921456337,
-0.0075959013774991035,
-0.027357902377843857,
0.02400708571076393,
-0.015116071328520775,
-0.019042329862713814,
-0.07426489144563675,
-0.01336245983839035,
-0.008157516829669476,
-0.0015594920841977,
0.0016904857475310564,
0.024369290098547935,
-0.006521725095808506,
0.03636253997683525,
-0.07173693925142288,
0.0066045126877725124,
0.024522876366972923,
0.013354518450796604,
0.05080261453986168,
-0.019749939441680908,
0.03684307262301445,
-0.0740196704864502,
0.036982275545597076,
0.011381049640476704,
-0.054732467979192734,
0.02818956971168518,
-0.019958559423685074,
-0.04414602369070053,
0.019125986844301224,
0.018952900543808937,
0.00609182333573699,
0.05315374955534935,
0.020350933074951172,
-0.026972852647304535,
0.11693400144577026,
-0.06226648390293121,
0.033191271126270294,
0.021454721689224243,
0.047024618834257126,
0.006136807147413492,
-0.05476098135113716,
-0.11355505138635635,
-0.002591314958408475,
-0.004215502180159092,
-0.03411300107836723,
-0.04484761506319046,
0.087701216340065,
0.030190560966730118,
0.05725317448377609,
-0.01477676909416914,
0.002997494535520673,
0.018355900421738625,
0.0023317327722907066,
0.02471359819173813,
0.0019562896341085434,
-0.05795745551586151,
-0.025587938725948334,
-0.060409266501665115,
0.025107169523835182,
-0.2771003246307373,
-0.01095799170434475,
-0.03920990601181984,
0.06808356940746307,
-0.01661461777985096,
-0.029192164540290833,
-0.005825720727443695,
-0.04671991243958473,
0.019523294642567635,
0.01083065290004015,
0.010356146842241287,
0.044418949633836746,
0.06089137867093086,
0.02134738303720951,
0.023069951683282852,
0.06980418413877487,
0.04191459342837334,
-0.06768660992383957,
0.012862715870141983,
-0.049695275723934174,
-0.0012920373119413853,
-0.04061688482761383,
0.15334850549697876,
0.05238231644034386,
-0.032179128378629684,
0.009234347380697727,
-0.02207128517329693,
-0.02865774929523468,
0.005704910960048437,
-0.03649754077196121,
0.04583205282688141,
-0.07069160789251328,
0.07444735616445541,
0.038866668939590454,
-0.003040639217942953,
0.052431073039770126,
-0.03310718387365341,
-0.009826084598898888,
-0.021322166547179222,
-0.010154765099287033,
-0.06499353796243668,
0.04186565428972244,
-0.03754819929599762,
0.021488051861524582,
0.010073245503008366,
-0.01561416033655405,
0.010958033613860607,
-0.08889424800872803,
-0.018663719296455383,
0.028993938118219376,
-0.06579014658927917,
-0.04132964089512825,
0.03754817694425583,
-0.013653569854795933,
0.020945081487298012,
-0.023982498794794083,
-0.017027970403432846,
-0.02793292887508869,
-0.011202839203178883,
0.03715185075998306,
0.05666804686188698,
0.00026263640029355884,
-0.02438916079699993,
0.07303771376609802,
0.000014487665794149507
] |
PMID:25010 | Use of gel precipitin test in the diagnosis of cytomegalovirus infections. | Cytomegalovirus (CMV) antibody was detected in human sera by the gel precipitin test. The results were closely related to those obtained by complement fixation. The simplicity of the test makes it useful for the routine serological diagnosis of CMV infection. | Use of gel precipitin test in the diagnosis of cytomegalovirus infections. Cytomegalovirus (CMV) antibody was detected in human sera by the gel precipitin test. The results were closely related to those obtained by complement fixation. The simplicity of the test makes it useful for the routine serological diagnosis of CMV infection. | [
-0.0705244168639183,
0.05052507668733597,
-0.024698957800865173,
0.022108981385827065,
0.04833907261490822,
-0.05747899413108826,
0.11666048318147659,
0.012329481542110443,
-0.013401923701167107,
-0.01892044022679329,
-0.005274543538689613,
-0.055837374180555344,
-0.014835287816822529,
-0.03493424132466316,
-0.070831298828125,
-0.04218319430947304,
0.05521206930279732,
0.01588553376495838,
-0.007673913612961769,
-0.00006196142203407362,
-0.1068761795759201,
-0.01628619246184826,
-0.035421643406152725,
-0.04344569519162178,
0.05978550389409065,
-0.010747586376965046,
-0.006928738672286272,
-0.024949155747890472,
-0.018845176324248314,
-0.13196726143360138,
0.04928999021649361,
-0.070488840341568,
0.017265865579247475,
-0.047250598669052124,
0.021267633885145187,
-0.024349018931388855,
0.026763256639242172,
-0.030774442479014397,
-0.014546332880854607,
0.016916746273636818,
0.13782162964344025,
-0.008041002787649632,
-0.08165975660085678,
-0.0069001903757452965,
0.030019143596291542,
-0.007790162228047848,
0.062156759202480316,
0.002223739866167307,
0.0879727229475975,
0.018353568390011787,
0.030599061399698257,
-0.04186909645795822,
0.05592240020632744,
0.05197184160351753,
-0.0550042986869812,
0.01843019388616085,
-0.037649430334568024,
-0.03193197026848793,
-0.031149806454777718,
0.011105059646070004,
-0.04633181542158127,
0.027179496362805367,
-0.1270214021205902,
0.006572573445737362,
0.03716682270169258,
0.0009160407353192568,
-0.04071700945496559,
-0.0625385046005249,
0.03254053369164467,
0.060879066586494446,
-0.06482947617769241,
0.03037618286907673,
-0.032830581068992615,
0.08895526081323624,
-0.004572496749460697,
-0.04269174858927727,
0.025602741166949272,
0.0016521234065294266,
0.04537862539291382,
0.034425992518663406,
-0.04757368564605713,
0.0809658020734787,
0.07613077014684677,
-0.038716595619916916,
0.03658406808972359,
0.017756979912519455,
0.021025635302066803,
0.005966871976852417,
0.04656982421875,
-0.04043138027191162,
0.01898498460650444,
-0.04052175208926201,
-0.04443513602018356,
0.058057110756635666,
-0.027177957817912102,
0.028841128572821617,
0.0795326977968216,
0.01797585003077984,
-0.002483394229784608,
0.3750135600566864,
-0.03778186812996864,
-0.029505286365747452,
-0.045994777232408524,
-0.016816774383187294,
0.0389007069170475,
-0.032023679465055466,
-0.0045607807114720345,
0.041850946843624115,
0.05290890112519264,
0.0060474565252661705,
0.022892573848366737,
-0.014178494922816753,
0.04553421214222908,
-0.014124516397714615,
-0.03071940876543522,
-0.03065527230501175,
0.04587613046169281,
0.028625613078475,
0.029194772243499756,
0.02806711010634899,
0.018804121762514114,
-0.04202398657798767,
0.03321259096264839,
-0.08393222093582153,
0.04624281823635101,
-0.017775461077690125,
0.03768661618232727,
0.10768306255340576,
-0.010387906804680824,
-0.014572083950042725,
0.04015378654003143,
0.022709593176841736,
-0.04344528540968895,
0.04201659560203552,
-0.016270142048597336,
0.008994541130959988,
0.006136707030236721,
-0.018109433352947235,
0.025844551622867584,
0.017368614673614502,
-0.022297251969575882,
-0.024804651737213135,
-0.05730119347572327,
-0.08732873946428299,
-0.040922269225120544,
0.07149653136730194,
-0.0819563940167427,
-0.02307230792939663,
0.03773825615644455,
-0.07588162273168564,
0.00909997895359993,
0.09233365952968597,
-0.018745461478829384,
0.07249291241168976,
0.03816543146967888,
0.0036345310509204865,
0.03902936354279518,
0.008176847361028194,
0.006691579706966877,
0.017354074865579605,
-0.001218509627506137,
-0.012128579430282116,
-0.054933879524469376,
0.06735363602638245,
-0.00425362354144454,
-0.0365065336227417,
-0.04895989969372749,
-0.06803184002637863,
0.03515808656811714,
-0.05030972138047218,
0.011764628812670708,
0.028216717764735222,
-0.0025201914831995964,
0.026603005826473236,
-0.022511959075927734,
-0.03673693165183067,
-0.09104815125465393,
0.06289781630039215,
0.03149629756808281,
0.03179483115673065,
0.026739617809653282,
0.07594258338212967,
-0.02380664087831974,
0.056588441133499146,
0.006402626167982817,
-0.01165339257568121,
0.016910618171095848,
0.0004200578841846436,
0.08366376906633377,
0.01074726227670908,
0.06370878219604492,
-0.009655737318098545,
-0.05537593364715576,
-0.014082469046115875,
-0.05192221701145172,
-0.003983392380177975,
-0.06513568013906479,
0.004245146177709103,
0.04688649997115135,
-0.026689747348427773,
0.027035245671868324,
-0.013126973994076252,
0.043544311076402664,
-0.010937581770122051,
-0.008508539758622646,
-0.027216970920562744,
0.026440229266881943,
-0.06271126866340637,
0.016390854492783546,
0.035526107996702194,
-0.0413520522415638,
0.05885425582528114,
-0.015058700926601887,
-0.0283841360360384,
-0.028193695470690727,
0.014003189280629158,
-0.04550404101610184,
-0.01249958947300911,
0.02452744171023369,
-0.018276361748576164,
-0.013790057972073555,
0.03309864550828934,
-0.0666828528046608,
-0.28744009137153625,
-0.014916079118847847,
-0.010504361242055893,
0.007196507882326841,
0.0011898899683728814,
-0.0030171971302479506,
0.0006632907898165286,
-0.03392094373703003,
0.013983143493533134,
-0.07638729363679886,
0.08069004863500595,
0.05470660701394081,
-0.08434347063302994,
-0.0507163442671299,
0.0009508483344689012,
0.05601535364985466,
-0.032114773988723755,
-0.008469218388199806,
0.0326121523976326,
0.004529900848865509,
-0.000023695856725680642,
-0.015841331332921982,
0.0304632019251585,
0.05282730981707573,
0.06573046743869781,
-0.06947456300258636,
0.10587042570114136,
0.06362693011760712,
-0.0394325852394104,
-0.05667857080698013,
0.00980628002434969,
0.03896934166550636,
0.01042118389159441,
-0.04042930155992508,
-0.009491979144513607,
0.06855867058038712,
-0.05219852551817894,
-0.014584283344447613,
-0.02844964526593685,
0.015328733250498772,
-0.022538987919688225,
-0.05081276223063469,
0.017050690948963165,
-0.00943110790103674,
0.0018536425195634365,
-0.002381916157901287,
0.032752349972724915,
0.04619022086262703,
0.017900515347719193,
0.034471504390239716,
-0.04280579462647438,
-0.0627637505531311,
-0.03995272517204285,
0.016703085973858833,
0.001899426570162177,
-0.04913204535841942,
-0.011529706418514252,
-0.039137497544288635,
-0.054683007299900055,
-0.004660059232264757,
0.0503196120262146,
-0.04910987615585327,
0.021269075572490692,
-0.03658422455191612,
-0.026173794642090797,
0.006298518739640713,
-0.03338492289185524,
-0.08319102972745895,
0.104347825050354,
-0.0023067740257829428,
-0.012976712547242641,
0.0949614942073822,
-0.03259115293622017,
0.01812657155096531,
0.04600278660655022,
-0.017925148829817772,
0.09982490539550781,
-0.054026246070861816,
-0.043817609548568726,
-0.022873762995004654,
0.03103615529835224,
-0.003200832987204194,
0.011050554923713207,
0.014666670002043247,
0.057895973324775696,
0.029429633170366287,
-0.012825751677155495,
0.03801130875945091,
-0.0422348752617836,
-0.05658392980694771,
0.00003697017746162601,
0.019552920013666153,
-0.011082333512604237,
-0.030520586296916008,
0.006069518160074949,
0.02190554514527321,
-0.2359272688627243,
0.06778167933225632,
-0.005586691666394472,
-0.009152581915259361,
0.05228603631258011,
-0.02433282881975174,
-0.005735993850976229,
-0.05838222801685333,
-0.03576689213514328,
0.05033393204212189,
0.04929491505026817,
-0.015544787049293518,
0.04368193447589874,
-0.00991372112184763,
0.00436636945232749,
0.06767063587903976,
0.08258262276649475,
-0.03481195122003555,
0.028069742023944855,
-0.024564025923609734,
0.02941097319126129,
-0.05292462185025215,
0.14833296835422516,
-0.003440829226747155,
-0.016829831525683403,
0.05738001689314842,
0.0028715000953525305,
-0.007774784695357084,
-0.011203132569789886,
0.011127605102956295,
0.026910362765192986,
-0.021140899509191513,
0.0189987663179636,
-0.019853979349136353,
0.04359079524874687,
0.04761867597699165,
-0.0015940532321110368,
0.014666818082332611,
0.017802683636546135,
0.01828937605023384,
-0.06616128236055374,
0.051856789737939835,
-0.025739261880517006,
-0.06022157147526741,
-0.004362521227449179,
-0.007486253976821899,
0.01148843765258789,
-0.05875247344374657,
0.0015469201607629657,
0.012958989478647709,
-0.0034499920438975096,
-0.04320613294839859,
0.02425539679825306,
-0.029845884069800377,
0.04743377864360809,
0.02285005897283554,
0.008099939674139023,
-0.041635628789663315,
0.024988410994410515,
-0.008091356605291367,
0.0023524172138422728,
-0.06666996330022812,
-0.01429657731205225,
0.09141191095113754,
-0.06699614226818085
] |
PMID:25011 | Preparation of rubella virus haemagglutinating antigen by polyethlene glycol precipitation. | Polyethylene glycol (PEG) was used for precipitation of rubella virus haemagglutinating (HA) antigen from the fluid phase of infected Vero cell cultures, maintained with or without calf serum. In both cases potent antigen preparation were obtained. When the antigen was precipitated from serum-containing medium, higher titres were obtained, provided that substrate or final product were treated with Tween 80 and ether. HA antigen obtained by PET precipitation was entirely valuable in the haemagglutination-inhibition test, as compared with antigens prepared by other methods. | Preparation of rubella virus haemagglutinating antigen by polyethlene glycol precipitation. Polyethylene glycol (PEG) was used for precipitation of rubella virus haemagglutinating (HA) antigen from the fluid phase of infected Vero cell cultures, maintained with or without calf serum. In both cases potent antigen preparation were obtained. When the antigen was precipitated from serum-containing medium, higher titres were obtained, provided that substrate or final product were treated with Tween 80 and ether. HA antigen obtained by PET precipitation was entirely valuable in the haemagglutination-inhibition test, as compared with antigens prepared by other methods. | [
-0.07045438140630722,
-0.0047718919813632965,
-0.005660834722220898,
-0.05069489777088165,
0.051609620451927185,
0.027004718780517578,
0.02605491131544113,
0.08401008695363998,
-0.05568056181073189,
0.0008744352962821722,
-0.009469258598983288,
-0.06392713636159897,
0.019198989495635033,
-0.049322161823511124,
-0.0751771479845047,
-0.031750090420246124,
0.0028245202265679836,
0.008543611504137516,
-0.05368844419717789,
0.03302943333983421,
-0.039330825209617615,
-0.034379925578832626,
-0.011840387247502804,
-0.018724866211414337,
0.04376358166337013,
0.007549651898443699,
0.01932527869939804,
0.037606384605169296,
-0.034935563802719116,
-0.1615743786096573,
-0.001214565010741353,
-0.054609354585409164,
-0.014279901050031185,
-0.06066790223121643,
-0.05537503957748413,
-0.016395846381783485,
-0.023037616163492203,
0.008866907097399235,
-0.06431911885738373,
-0.005955467466264963,
0.027198441326618195,
-0.013311777263879776,
-0.10465549677610397,
0.005767453461885452,
-0.015515796840190887,
-0.020074717700481415,
-0.03355637937784195,
0.024481425061821938,
0.07603855431079865,
0.00046662805834785104,
0.004303035791963339,
0.06324152648448944,
0.008279027417302132,
-0.031803157180547714,
-0.04909559711813927,
0.005675092339515686,
-0.03830812871456146,
0.013260076753795147,
-0.04282340779900551,
-0.03927790746092796,
-0.03885701298713684,
0.03534035384654999,
-0.10893037915229797,
0.07750293612480164,
0.02085021324455738,
-0.013750521466135979,
-0.01050500851124525,
-0.021908853203058243,
0.07012850791215897,
0.046582434326410294,
-0.03926749899983406,
0.07572072744369507,
-0.008313342928886414,
0.06467084586620331,
-0.05738518387079239,
-0.0075376913882792,
-0.010699760168790817,
-0.0035173571668565273,
0.085676409304142,
0.04415597394108772,
-0.04116368293762207,
0.07120232284069061,
0.020577047020196915,
-0.019956255331635475,
-0.052833542227745056,
0.022316550835967064,
-0.004299731459468603,
-0.0026203705929219723,
0.08097510784864426,
0.012022946961224079,
0.04493538290262222,
-0.00535360723733902,
-0.04896887391805649,
0.04408472031354904,
-0.0706961378455162,
0.025643572211265564,
-0.0013119939249008894,
-0.032250966876745224,
0.044231656938791275,
0.4091745615005493,
-0.05804607644677162,
-0.04910785332322121,
0.027351269498467445,
-0.01438128761947155,
-0.01950918883085251,
-0.027367062866687775,
-0.026273388415575027,
0.03128973767161369,
-0.011145719327032566,
0.033486414700746536,
-0.016536440700292587,
0.04069415107369423,
0.020298387855291367,
-0.043362732976675034,
0.0013595697237178683,
-0.010409096255898476,
-0.015121116302907467,
0.025262953713536263,
-0.035586897283792496,
0.0120571069419384,
0.02029225416481495,
0.007971502840518951,
0.0447506383061409,
-0.05182862654328346,
0.038171228021383286,
0.02849971130490303,
-0.009310628287494183,
0.12201191484928131,
0.00048689084360376,
0.0011208062060177326,
0.021832792088389397,
0.00017150210624095052,
-0.03647758066654205,
-0.03854122385382652,
0.04962996393442154,
0.040312740951776505,
0.03460002690553665,
0.034037914127111435,
0.030582617968320847,
0.02555624023079872,
-0.0657629445195198,
-0.015497266314923763,
-0.05979185178875923,
-0.04215221479535103,
0.00887511856853962,
0.0348028838634491,
-0.07093147933483124,
0.06311950087547302,
-0.005704159382730722,
-0.052538853138685226,
0.005851114168763161,
0.032735683023929596,
-0.04527844488620758,
0.006676116026937962,
0.01955687627196312,
-0.013274562545120716,
-0.0005288806278258562,
0.023974206298589706,
-0.010812078602612019,
0.005732008721679449,
-0.05207376927137375,
-0.03973965346813202,
0.04120073840022087,
0.04286514222621918,
-0.049849726259708405,
-0.038126666098833084,
-0.00643949257209897,
-0.02739080972969532,
0.053342122584581375,
-0.05611540377140045,
-0.008159693330526352,
0.017003940418362617,
-0.03887118771672249,
0.020428573712706566,
-0.015127680264413357,
-0.039188068360090256,
-0.030176537111401558,
0.05268966406583786,
0.009035123512148857,
0.020820505917072296,
0.029170889407396317,
0.0141125014051795,
-0.005664034280925989,
0.0314037948846817,
0.03112957254052162,
-0.0318136066198349,
-0.01922823302447796,
0.04534715414047241,
-0.005034628789871931,
0.04400546848773956,
-0.024364586919546127,
0.0075829545967280865,
-0.051076989620923996,
0.03762948885560036,
0.04927782714366913,
0.005595693830400705,
-0.045392461121082306,
0.0051240259781479836,
0.06991268694400787,
-0.029903724789619446,
0.09477271884679794,
-0.022369984537363052,
0.0032244890462607145,
0.00005258137025521137,
0.023145506158471107,
0.03336083143949509,
0.11692625284194946,
-0.038308847695589066,
0.049212634563446045,
0.024066315963864326,
-0.02534044347703457,
0.049159422516822815,
-0.04527648910880089,
0.01322020124644041,
-0.05281563475728035,
0.06158178672194481,
-0.019987594336271286,
-0.02173531986773014,
0.026907388120889664,
-0.011082477867603302,
0.061417464166879654,
-0.02194216102361679,
-0.036786772310733795,
-0.28232255578041077,
0.0038262279704213142,
0.017988819628953934,
-0.0517418198287487,
0.052258897572755814,
0.007707351818680763,
-0.027479758486151695,
-0.032632794231176376,
0.02434075064957142,
-0.03833501785993576,
0.09914913773536682,
-0.003073409665375948,
-0.016628960147500038,
-0.0479457750916481,
-0.021515831351280212,
0.07535525411367416,
-0.03446343168616295,
0.014856676571071148,
0.04870910942554474,
0.0007524594548158348,
0.013573689386248589,
-0.007548597641289234,
0.018731610849499702,
0.07357903569936752,
0.03828035667538643,
-0.038651417940855026,
0.0981491208076477,
0.022502386942505836,
-0.001764299813657999,
-0.08089753985404968,
0.016744788736104965,
0.04997875168919563,
-0.010032263584434986,
-0.06522031128406525,
-0.003250361420214176,
0.041247058659791946,
-0.075472392141819,
0.044875286519527435,
0.030795181170105934,
0.00854650977998972,
0.061664409935474396,
-0.0018212997820228338,
-0.00966321025043726,
-0.08475338667631149,
0.03251892328262329,
-0.023060331121087074,
-0.0169998612254858,
0.02052101120352745,
0.0535898357629776,
0.006865326315164566,
0.06267113238573074,
-0.10916103422641754,
0.016911432147026062,
0.02567611075937748,
0.03276169300079346,
-0.006739222910255194,
0.0022859093733131886,
0.04066161811351776,
-0.0685504749417305,
0.028462648391723633,
0.08291057497262955,
-0.047132931649684906,
-0.02167266048491001,
0.02213321253657341,
-0.05786609649658203,
0.0015334178460761905,
0.034476663917303085,
-0.05036722868680954,
0.08890458941459656,
0.0056368340738117695,
-0.0631188154220581,
0.09167291969060898,
-0.018676523119211197,
0.061995234340429306,
0.03362689167261124,
0.05941455066204071,
-0.051896508783102036,
-0.02749081701040268,
-0.03676572069525719,
-0.031968388706445694,
0.04659358039498329,
0.007146934513002634,
0.022549429908394814,
0.027079051360487938,
0.027691345661878586,
0.006895268335938454,
-0.04669461026787758,
-0.008225937373936176,
-0.0100974440574646,
-0.00015331734903156757,
0.0016601174138486385,
-0.02247599884867668,
-0.04099239036440849,
-0.04273935407400131,
-0.03779488429427147,
-0.0027950117364525795,
-0.2676292657852173,
0.026702167466282845,
0.010679414495825768,
0.024604374542832375,
0.012975426390767097,
0.004242295399308205,
-0.000981289311312139,
-0.06284589320421219,
0.024360114708542824,
0.027398938313126564,
-0.0005218083970248699,
0.023970311507582664,
0.026642832905054092,
-0.0014727268135175109,
0.04216109588742256,
0.0325138084590435,
0.005890099331736565,
-0.03384314104914665,
-0.0014879741938784719,
-0.042201895266771317,
0.05257347598671913,
-0.08994819223880768,
0.15106536448001862,
-0.0006338395760394633,
-0.05975520238280296,
0.03355199098587036,
-0.003889900865033269,
-0.023538514971733093,
-0.04707740992307663,
0.005849949084222317,
0.04043613746762276,
0.0414879247546196,
0.07614828646183014,
-0.013430868275463581,
0.0064583527855575085,
-0.009134850464761257,
0.009041515178978443,
0.04757118225097656,
-0.0058127823285758495,
0.04889072850346565,
-0.04230829328298569,
0.049407362937927246,
0.02406449243426323,
-0.06477871537208557,
0.022369930520653725,
-0.01348969992250204,
-0.03919650986790657,
-0.08896832168102264,
-0.032019346952438354,
-0.050727762281894684,
0.0008475988288410008,
-0.07186324149370193,
0.03840704634785652,
-0.018226383253932,
-0.0007059801719151437,
-0.05034114047884941,
0.018822675570845604,
0.03544546663761139,
0.02862383984029293,
0.03611420467495918,
0.009019079618155956,
-0.02659647911787033,
-0.012613466009497643,
0.09645508974790573,
-0.011101914569735527
] |
PMID:25012 | Isolation of Tettnang virus from Ixodes ricinus ticks in Czechoslovakia. | Three virus strains closely related to Tettnang virus were isolated from nymphs and males of Ixodes ricinus tick in Moravia and Slovakia. The virus isolates were lethal for suckling mice on intracerebral (i.c.) inoculation but the virus titres in the brain were low. The incubation period of 5--12 days in the first mouse passage was shortened to 3 days in further passages. Infection of mice was accompanied by ultrastructural changes in the cytoplasm of neurons. Groups of spherical virus particles 75 to 80 nm in diameter occurred within simple vacuoles. | Isolation of Tettnang virus from Ixodes ricinus ticks in Czechoslovakia. Three virus strains closely related to Tettnang virus were isolated from nymphs and males of Ixodes ricinus tick in Moravia and Slovakia. The virus isolates were lethal for suckling mice on intracerebral (i.c.) inoculation but the virus titres in the brain were low. The incubation period of 5--12 days in the first mouse passage was shortened to 3 days in further passages. Infection of mice was accompanied by ultrastructural changes in the cytoplasm of neurons. Groups of spherical virus particles 75 to 80 nm in diameter occurred within simple vacuoles. | [
0.0438619889318943,
-0.02940112166106701,
-0.005587530788034201,
0.003978670109063387,
0.032196078449487686,
-0.056778404861688614,
0.03760162740945816,
0.0368325375020504,
-0.03114137053489685,
-0.045733191072940826,
0.011844048276543617,
-0.07961995899677277,
0.014722185209393501,
-0.010448317043483257,
-0.034659501165151596,
-0.07586438208818436,
-0.05422203242778778,
-0.09071444720029831,
0.017052432522177696,
0.040345847606658936,
-0.033820319920778275,
-0.030933290719985962,
0.017161814495921135,
-0.060623299330472946,
-0.00045537532423622906,
0.01262533850967884,
0.016071708872914314,
-0.008518689312040806,
-0.0030890635680407286,
-0.16833628714084625,
-0.0018132212571799755,
-0.03207775205373764,
-0.0687045156955719,
-0.0027163841295987368,
-0.009995798580348492,
-0.006768631283193827,
-0.024132395163178444,
-0.04869535565376282,
-0.005010444205254316,
-0.0010212571360170841,
0.03918018564581871,
0.07886935025453568,
-0.006805763114243746,
-0.01289181038737297,
0.03764248639345169,
-0.005503206979483366,
-0.008805489167571068,
-0.009533762000501156,
0.08025044202804565,
0.016357509419322014,
-0.010705748572945595,
-0.02696371264755726,
0.038132939487695694,
0.021831005811691284,
-0.03225065395236015,
-0.010595669969916344,
-0.007365962490439415,
0.0207282155752182,
0.00805299635976553,
0.056253138929605484,
-0.02289477549493313,
0.05810107663273811,
-0.0957471951842308,
0.05876629799604416,
0.02532920241355896,
-0.000022466369046014734,
-0.04286418855190277,
0.027529237791895866,
0.02559710294008255,
0.03397083282470703,
-0.014177714474499226,
0.09738186746835709,
-0.0015615281881764531,
-0.009083304554224014,
0.02496170811355114,
0.02148646116256714,
-0.009090591222047806,
0.0042740157805383205,
0.0478743351995945,
0.019480079412460327,
0.0012482971651479602,
0.027187947183847427,
0.0727342739701271,
0.016562795266509056,
-0.014163877815008163,
-0.0027085861656814814,
-0.020703928545117378,
-0.04769092798233032,
-0.02908174879848957,
-0.011928458698093891,
-0.012044436298310757,
0.00032188877230510116,
-0.002091997303068638,
-0.06717059761285782,
-0.0678306370973587,
-0.011320357210934162,
0.0880868211388588,
0.014798273332417011,
0.029409589245915413,
0.35714563727378845,
-0.01816732995212078,
-0.020942680537700653,
-0.019634367898106575,
-0.007929445244371891,
-0.0016719436971470714,
0.027217715978622437,
-0.05436645820736885,
-0.019486797973513603,
-0.024782337248325348,
0.054780263453722,
0.04066864401102066,
0.02676665596663952,
-0.01340685598552227,
0.055156633257865906,
0.05957968160510063,
-0.09992347657680511,
-0.02695162035524845,
-0.017556771636009216,
-0.033481165766716,
0.05586446076631546,
-0.0016963747330009937,
-0.012686793692409992,
0.04345913231372833,
-0.03862668573856354,
0.07326186448335648,
0.03997175395488739,
-0.001454694545827806,
0.08261378854513168,
0.07912012189626694,
-0.040714047849178314,
0.032684076577425,
-0.005796517711132765,
-0.055277854204177856,
0.009718856774270535,
0.014475846663117409,
-0.007573301438242197,
-0.004675798583775759,
0.0039250836707651615,
0.0015320938546210527,
-0.04205776005983353,
-0.040142424404621124,
-0.09937520325183868,
0.01790926605463028,
-0.032101403921842575,
-0.044743962585926056,
-0.005332012660801411,
-0.033918194472789764,
-0.039359916001558304,
-0.0014196103438735008,
0.027762500569224358,
0.027195598930120468,
0.07076302915811539,
-0.026751063764095306,
-0.01080461498349905,
0.019500598311424255,
-0.021856535226106644,
0.022969216108322144,
0.031045930460095406,
0.0023385349195450544,
-0.03098107874393463,
-0.04699193686246872,
-0.0544520802795887,
-0.004392505623400211,
0.059087883681058884,
0.01590178720653057,
-0.06243489682674408,
0.02729877084493637,
0.007048421539366245,
0.08410404622554779,
-0.07865259796380997,
-0.0007412381819449365,
-0.04128718003630638,
-0.005321433302015066,
0.043341245502233505,
-0.0643298551440239,
-0.057490669190883636,
-0.03000621497631073,
-0.02670462243258953,
0.023557523265480995,
0.057760462164878845,
0.0026591031346470118,
-0.009238192811608315,
-0.026419829577207565,
0.07570847868919373,
-0.012654384598135948,
0.0021007307805120945,
0.010059538297355175,
0.0010735158575698733,
-0.019492799416184425,
0.0018277033232152462,
-0.013551666401326656,
-0.0444798469543457,
0.07901518791913986,
-0.0243073720484972,
0.0029928525909781456,
0.02026880346238613,
0.03384561091661453,
0.030419277027249336,
0.06197001412510872,
-0.04768863320350647,
0.0779603123664856,
-0.07075905054807663,
-0.00519923260435462,
0.04345384240150452,
-0.02302020974457264,
0.07568861544132233,
0.05932653322815895,
-0.08236169070005417,
0.007383758667856455,
-0.005655872169882059,
0.010700293816626072,
0.0019678431563079357,
-0.010470904409885406,
-0.042095258831977844,
-0.033834218978881836,
0.10566165298223495,
-0.011658421717584133,
-0.02659560553729534,
0.05631879344582558,
0.057179179042577744,
0.03806198760867119,
-0.020690815523266792,
-0.017280369997024536,
-0.2495499551296234,
-0.08550358563661575,
-0.011415068991482258,
-0.04476306959986687,
-0.0517999641597271,
-0.08211639523506165,
0.02362637408077717,
-0.05541640892624855,
0.06682126969099045,
-0.032305970788002014,
0.006676679942756891,
0.0653589740395546,
0.02393774501979351,
-0.052745796740055084,
0.008454013615846634,
0.126692533493042,
0.04717778414487839,
0.0670514851808548,
0.028017494827508926,
-0.03399121016263962,
0.033390067517757416,
0.023763811215758324,
0.03531063720583916,
0.0057923560962080956,
0.013255455531179905,
-0.05625937506556511,
0.16282905638217926,
0.02007478103041649,
0.00565171567723155,
-0.06810180097818375,
-0.04064735397696495,
0.018829714506864548,
0.020296921953558922,
-0.058821965008974075,
-0.014367667026817799,
-0.005702699068933725,
-0.017042599618434906,
0.008456542156636715,
0.02559930644929409,
-0.07735822349786758,
0.003008690197020769,
-0.005083413794636726,
-0.013990821316838264,
-0.027510475367307663,
0.03277306631207466,
-0.011582172475755215,
-0.044876646250486374,
0.015063603408634663,
0.05777565389871597,
0.009208278730511665,
0.017517181113362312,
-0.01571001298725605,
0.04857560619711876,
0.05200372636318207,
-0.021134531125426292,
0.040097519755363464,
-0.05634396895766258,
0.02892559953033924,
-0.024683257564902306,
0.054189011454582214,
-0.013563692569732666,
-0.03737880662083626,
-0.01758357509970665,
-0.009595508687198162,
-0.025776464492082596,
0.014189987443387508,
0.014474648050963879,
-0.04660835117101669,
0.12392165511846542,
0.05654625967144966,
-0.03787251189351082,
0.08417091518640518,
0.018085399642586708,
-0.041202887892723083,
-0.07286272197961807,
0.03479265794157982,
-0.039753589779138565,
-0.018143191933631897,
-0.046063825488090515,
0.002151468303054571,
-0.010123718529939651,
-0.046051397919654846,
0.04401438310742378,
0.018155492842197418,
-0.0161562729626894,
-0.012949317693710327,
-0.006924740970134735,
0.02656967006623745,
0.017709944397211075,
0.019669245928525925,
0.05664186552166939,
-0.07672876864671707,
-0.05400580167770386,
-0.01140037551522255,
0.0004924794193357229,
0.05403495579957962,
-0.2765551507472992,
-0.005891179665923119,
0.035195719450712204,
0.01693715527653694,
0.06473813951015472,
0.004967173095792532,
0.015131459571421146,
-0.05530501529574394,
-0.023695794865489006,
-0.011908948421478271,
0.06321293860673904,
0.0038815399166196585,
0.009326807223260403,
0.015502492897212505,
0.003603433258831501,
0.06343657523393631,
0.004349938593804836,
-0.01249806210398674,
0.03064143657684326,
-0.03706701844930649,
-0.0533546507358551,
-0.07026427239179611,
0.17257575690746307,
0.055614691227674484,
-0.021089250221848488,
-0.0065533616580069065,
0.017354294657707214,
0.024515999481081963,
0.013908368535339832,
-0.06980562955141068,
-0.003125519957393408,
-0.04220285639166832,
0.042437244206666946,
-0.032261405140161514,
0.04725120961666107,
0.10889773070812225,
-0.007845588028430939,
0.008281123824417591,
0.023860102519392967,
-0.012492693960666656,
-0.0549805648624897,
0.02817666158080101,
-0.013317713513970375,
0.002364181913435459,
-0.003173388075083494,
-0.022386299446225166,
-0.06794950366020203,
-0.13503028452396393,
-0.04030485451221466,
0.04076395556330681,
0.0057508875615894794,
-0.047578390687704086,
0.04216287285089493,
-0.015082531608641148,
0.02388647198677063,
0.04115677624940872,
0.060827575623989105,
-0.032788507640361786,
-0.0016889470862224698,
-0.00380249647423625,
0.03817347437143326,
-0.06715729832649231,
-0.02159818820655346,
0.09127838909626007,
0.05301002785563469
] |
PMID:25016 | The nature of the renal response to chronic disorders of acid-base equilibrium. | The rate of acid excretion by the kidney appears to be determined by factors regulating the site and the rate of sodium reabsorption, rather than by a homeostatic mechanism that responds to systemic pH. This hypothesis, although unconventional, is supported by much experimental evidence, and it accounts for a wide variety of clinical and physiologic findings that heretofore have been difficult or impossible to explain. | The nature of the renal response to chronic disorders of acid-base equilibrium. The rate of acid excretion by the kidney appears to be determined by factors regulating the site and the rate of sodium reabsorption, rather than by a homeostatic mechanism that responds to systemic pH. This hypothesis, although unconventional, is supported by much experimental evidence, and it accounts for a wide variety of clinical and physiologic findings that heretofore have been difficult or impossible to explain. | [
-0.017227910459041595,
0.001780874328687787,
0.02925165370106697,
0.06837195158004761,
0.0017197213601320982,
-0.060909900814294815,
0.11212888360023499,
0.024513859301805496,
-0.02293229289352894,
-0.019454970955848694,
0.0692402720451355,
-0.08241195231676102,
0.04613211750984192,
0.08312028646469116,
0.0033745907712727785,
0.008980877697467804,
0.003581575583666563,
0.041986022144556046,
-0.025767464190721512,
-0.016958026215434074,
0.052681814879179,
0.004116983152925968,
0.014403960667550564,
-0.03314433619379997,
0.013327617198228836,
-0.05343974754214287,
0.02772415243089199,
-0.03974093496799469,
-0.07181399315595627,
-0.1460467427968979,
-0.04121766984462738,
-0.001839936594478786,
0.03133949264883995,
-0.017322994768619537,
-0.022579725831747055,
-0.02109318971633911,
-0.041034240275621414,
0.001758993836119771,
-0.022760463878512383,
0.056886520236730576,
0.012754536233842373,
0.020616276189684868,
0.023494234308600426,
0.029304835945367813,
-0.008296002633869648,
0.01913890242576599,
-0.0023348547983914614,
0.014186995103955269,
0.05986621230840683,
0.08950769901275635,
-0.020387612283229828,
0.024996276944875717,
0.027961036190390587,
0.09414105862379074,
0.012293273583054543,
-0.00506707513704896,
0.019117435440421104,
-0.0010666585294529796,
0.048901401460170746,
-0.0329805351793766,
0.013996541500091553,
0.054483603686094284,
-0.11928126215934753,
0.07588072121143341,
0.06925563514232635,
0.05980505421757698,
-0.06593476980924606,
0.01767069846391678,
0.034782882779836655,
-0.01185357104986906,
-0.04851582646369934,
0.07057361304759979,
-0.03251653537154198,
-0.0277550145983696,
0.06131777912378311,
-0.021396325901150703,
0.01949435845017433,
-0.003607803490012884,
0.0068482086062431335,
0.010689422488212585,
0.04003532975912094,
0.027126096189022064,
0.023392708972096443,
0.019602423533797264,
0.02150610089302063,
-0.0738251656293869,
0.07718656957149506,
0.009014395996928215,
0.0011775761377066374,
0.024714168161153793,
-0.04235420748591423,
-0.055761877447366714,
-0.02704787626862526,
0.01134444959461689,
-0.015725018456578255,
0.035627976059913635,
0.015538477338850498,
-0.03788182511925697,
-0.05133131146430969,
0.34842079877853394,
-0.03696423023939133,
0.0951695442199707,
-0.09028071165084839,
-0.008984100073575974,
0.03503275662660599,
-0.07032447308301926,
-0.03045756369829178,
0.009012222290039062,
-0.06551344692707062,
0.03468611463904381,
0.014028484001755714,
0.04248986765742302,
0.04954522103071213,
-0.06792464852333069,
-0.032762154936790466,
0.023130454123020172,
0.007508226204663515,
0.02357320487499237,
0.012095022015273571,
0.004967821761965752,
0.008808912709355354,
0.023603364825248718,
-0.00769813172519207,
-0.07015757262706757,
0.052883755415678024,
0.00262910732999444,
0.0295103769749403,
0.066736601293087,
0.01867319457232952,
0.007572854869067669,
0.047280482947826385,
-0.01776927150785923,
-0.053462326526641846,
-0.02753525972366333,
-0.02723151631653309,
-0.0025187754072248936,
0.03840271756052971,
-0.06563537567853928,
0.004962863400578499,
0.055445291101932526,
-0.07018314301967621,
-0.038509078323841095,
-0.07774142175912857,
-0.07519134134054184,
-0.12526105344295502,
0.1248023733496666,
0.046052977442741394,
0.021851668134331703,
-0.026851879432797432,
0.013349976390600204,
-0.004499735310673714,
0.03984770178794861,
-0.024205008521676064,
-0.00003021821794391144,
-0.027298998087644577,
0.053005825728178024,
-0.040936149656772614,
-0.031662359833717346,
0.018305737525224686,
-0.007212398573756218,
-0.03029453381896019,
-0.054582808166742325,
-0.010710461065173149,
0.028984490782022476,
0.01918342150747776,
0.02846871316432953,
-0.047885216772556305,
-0.045963626354932785,
0.06939660757780075,
0.016968199983239174,
-0.026730285957455635,
-0.010931523516774178,
0.0018505698535591364,
-0.08800987899303436,
-0.02196362614631653,
-0.0324539840221405,
-0.018477488309144974,
0.015777237713336945,
-0.024212120100855827,
-0.010381028987467289,
0.042822714895009995,
0.05002289637923241,
-0.018403686583042145,
0.018700119107961655,
0.010575616732239723,
-0.03167552873492241,
-0.04527880623936653,
-0.011393947526812553,
-0.003451765049248934,
0.03426333889365196,
0.00555643392726779,
0.007761271204799414,
-0.06120990961790085,
-0.011074837297201157,
-0.012831583619117737,
-0.0034089095424860716,
0.02834424562752247,
-0.00026947574224323034,
-0.02932197041809559,
0.06130361184477806,
0.033340875059366226,
0.014148873277008533,
-0.03695327416062355,
0.06603068113327026,
-0.01186063326895237,
0.021589869633316994,
0.007818232290446758,
-0.006215629633516073,
-0.006272383499890566,
0.05972921475768089,
-0.025794994086027145,
0.015104902908205986,
-0.06399188935756683,
-0.08304115384817123,
-0.01589670591056347,
-0.005237744655460119,
0.03205537050962448,
0.0047472394071519375,
0.09649664163589478,
-0.029802493751049042,
0.049844466149806976,
-0.05784779414534569,
-0.0028252783231437206,
-0.2935757637023926,
0.009324750863015652,
0.04565845802426338,
-0.014933132566511631,
0.10871953517198563,
-0.05078212916851044,
-0.005527116823941469,
-0.018243517726659775,
-0.06750794500112534,
-0.020195629447698593,
0.020864389836788177,
0.022740274667739868,
-0.028919249773025513,
-0.036881376057863235,
-0.019237391650676727,
0.017100991681218147,
0.03271445631980896,
-0.03410286828875542,
0.039697423577308655,
-0.023766960948705673,
0.06304723024368286,
-0.06946855783462524,
0.026733940467238426,
-0.014349921606481075,
0.027952414005994797,
0.0008386804256588221,
0.09933531284332275,
-0.04178042337298393,
-0.024835431948304176,
0.014239083975553513,
0.07185126096010208,
-0.013485603965818882,
0.01212421152740717,
-0.07710962742567062,
0.0018804047722369432,
-0.020795732736587524,
-0.008676177822053432,
-0.04249073192477226,
0.00847780704498291,
-0.05002255737781525,
-0.06024395301938057,
-0.00019425922073423862,
-0.039068710058927536,
0.06294426321983337,
-0.09656283259391785,
0.014475284144282341,
0.022560223937034607,
0.09307575970888138,
-0.016668295487761497,
-0.07208047807216644,
0.03687508776783943,
-0.01076427660882473,
-0.006629077717661858,
-0.04211597144603729,
0.03942425549030304,
0.024183616042137146,
-0.013694165274500847,
-0.03305019810795784,
-0.07996053993701935,
-0.024458348751068115,
0.007290629670023918,
0.05760207772254944,
0.04921330139040947,
0.003949552774429321,
0.007099353242665529,
0.01580614037811756,
-0.027615610510110855,
0.04769573360681534,
0.04264316335320473,
0.015708651393651962,
0.019676202908158302,
0.12970635294914246,
-0.027196884155273438,
0.06602050364017487,
0.06097646430134773,
0.04733454808592796,
-0.03716248273849487,
-0.032236676663160324,
-0.03840098902583122,
-0.028815168887376785,
-0.002402890007942915,
-0.11627738177776337,
0.05026128143072128,
0.006582848262041807,
0.018182070925831795,
0.01473988126963377,
0.031107963994145393,
-0.04189737141132355,
0.014683176763355732,
0.014788402244448662,
-0.04288032650947571,
0.015435710549354553,
-0.041459280997514725,
-0.022941764444112778,
-0.030723078176379204,
0.054822780191898346,
-0.2848533093929291,
0.019534381106495857,
0.031278952956199646,
-0.002699808683246374,
-0.013147118501365185,
0.06751997023820877,
-0.01807870715856552,
0.03688257932662964,
-0.018725158646702766,
0.02788073755800724,
0.05114671587944031,
-0.02709774672985077,
0.011004123836755753,
0.02503982186317444,
-0.008046355098485947,
-0.04288645461201668,
0.06222345307469368,
-0.05803394690155983,
0.004597686231136322,
-0.024997694417834282,
0.02423527091741562,
0.021138321608304977,
0.14227817952632904,
-0.01000287476927042,
0.02040785923600197,
0.0394354946911335,
-0.030712110921740532,
0.014908960089087486,
-0.07536985725164413,
-0.02808361127972603,
0.017953157424926758,
0.040415871888399124,
-0.010707934387028217,
0.008029580116271973,
-0.0026898193173110485,
-0.015846572816371918,
-0.03220198675990105,
0.02256038784980774,
-0.006389336660504341,
0.022550225257873535,
0.005976137705147266,
-0.011348175816237926,
-0.05553213506937027,
-0.04624094441533089,
0.051050808280706406,
-0.05094708502292633,
-0.05399204045534134,
-0.059394244104623795,
0.028333883732557297,
0.020311923697590828,
-0.010623877868056297,
-0.050989676266908646,
0.05925847589969635,
0.040870506316423416,
0.006503432523459196,
-0.02298029139637947,
-0.01677851378917694,
-0.07718285173177719,
-0.000747614074498415,
-0.042438458651304245,
0.04188667610287666,
0.05708930268883705,
0.029430769383907318,
0.011869232170283794,
0.045764680951833725
] |
PMID:25017 | Sympathetic responsiveness and antihypertensive effect of beta-receptor blockade in essential hypertension. | The relationship between sympathetic responsiveness and the blood pressure reduction induced by long-term beta-blockade was assessed in patients with essential hypertension. The increase in plasma noradrenaline concentration during physical exercise was used as an index of sympathetic responsiveness. The cardioselective beta-blocker, atenolol, was given to 16 patients with sustained benign essential hypertension for five weeks at a dose of 200 mg/day. Atenolol induced a marked decrease in blood pressure and pulse rate during recumbency, orthostasis and exercise concomitant with a marked increase in plasma noradrenaline concentration (p less than 0.0125) and a pronounced decrease in plasma renin concentration (p less than 0.01). The ratio of plasma noradrenaline during exercise to the base line concentration correlated significantly with the subsequent decrease in mean arterial blood pressure induced by beta-blockade (r = 0.840; p less than 0.001). A less significant correlation was observed between the plasma renin concentration and the subsequent decrease in mean arterial pressure (r = 0.542; p less than 0.05). The results obtained indicate that sympathetic responsiveness is an important determinant of blood pressure response to beta-blockade induced by atenolol. | Sympathetic responsiveness and antihypertensive effect of beta-receptor blockade in essential hypertension. The relationship between sympathetic responsiveness and the blood pressure reduction induced by long-term beta-blockade was assessed in patients with essential hypertension. The increase in plasma noradrenaline concentration during physical exercise was used as an index of sympathetic responsiveness. The cardioselective beta-blocker, atenolol, was given to 16 patients with sustained benign essential hypertension for five weeks at a dose of 200 mg/day. Atenolol induced a marked decrease in blood pressure and pulse rate during recumbency, orthostasis and exercise concomitant with a marked increase in plasma noradrenaline concentration (p less than 0.0125) and a pronounced decrease in plasma renin concentration (p less than 0.01). The ratio of plasma noradrenaline during exercise to the base line concentration correlated significantly with the subsequent decrease in mean arterial blood pressure induced by beta-blockade (r = 0.840; p less than 0.001). A less significant correlation was observed between the plasma renin concentration and the subsequent decrease in mean arterial pressure (r = 0.542; p less than 0.05). The results obtained indicate that sympathetic responsiveness is an important determinant of blood pressure response to beta-blockade induced by atenolol. | [
-0.03309183567762375,
0.07174213975667953,
0.03817840665578842,
0.04943583533167839,
-0.017144693061709404,
-0.025185517966747284,
0.044245898723602295,
0.0037732673808932304,
-0.04936109110713005,
-0.06393232196569443,
-0.004136189818382263,
-0.02015363797545433,
0.02028067782521248,
0.054236989468336105,
-0.03358607366681099,
-0.009659621864557266,
0.0746862068772316,
-0.0007931500440463424,
-0.04414844140410423,
-0.00706496462225914,
-0.02195676974952221,
-0.020304998382925987,
0.07712028175592422,
-0.007821433246135712,
0.0644548237323761,
-0.023362113162875175,
-0.022749759256839752,
0.010633615776896477,
-0.10435887426137924,
-0.17149364948272705,
0.0007104856194928288,
-0.011458133347332478,
0.047400519251823425,
0.012797635979950428,
-0.014091799966990948,
0.017351526767015457,
-0.058039385825395584,
0.009300283156335354,
-0.03521811217069626,
0.025811292231082916,
0.03978309780359268,
0.005041276570409536,
0.004354647360742092,
0.04942077770829201,
0.009670794010162354,
-0.0048849876038730145,
-0.016435656696558,
0.00040678991354070604,
0.07292692363262177,
0.013713560067117214,
-0.03415962681174278,
0.017476657405495644,
-0.017932124435901642,
0.06208603456616402,
0.04205676540732384,
0.010122239589691162,
-0.020850839093327522,
0.05407106131315231,
0.014123368076980114,
0.010791640728712082,
-0.01815437525510788,
0.08016066998243332,
-0.13765652477741241,
0.07522295415401459,
0.028163239359855652,
0.03366929292678833,
-0.04064318165183067,
-0.005759961437433958,
0.029824981465935707,
0.06850231438875198,
0.0042724609375,
0.07627017796039581,
-0.011702320538461208,
-0.015114291571080685,
-0.0012276044581085443,
-0.009423773735761642,
-0.04401390999555588,
0.014330410398542881,
-0.006027458235621452,
-0.00833813939243555,
0.04515125975012779,
-0.029849985614418983,
-0.03344755619764328,
-0.03235198184847832,
0.036161888390779495,
-0.05865352228283882,
0.09124074876308441,
-0.036756712943315506,
0.00886384304612875,
-0.0012064565671607852,
-0.04190909117460251,
-0.04582500457763672,
-0.014185722917318344,
0.021625012159347534,
0.04476749151945114,
-0.012918454594910145,
-0.028092147782444954,
-0.050301581621170044,
-0.06947305053472519,
0.3642762005329132,
0.02693617157638073,
0.0609714649617672,
-0.10112211108207703,
0.015977773815393448,
0.00007887483661761507,
-0.050570059567689896,
-0.004130272660404444,
0.02179567702114582,
0.0006428582710213959,
0.006639075465500355,
0.010262907482683659,
-0.0353059321641922,
0.08744842559099197,
-0.05728944391012192,
-0.014715606346726418,
-0.028993329033255577,
0.028386017307639122,
-0.02749778889119625,
0.023642055690288544,
0.03668208420276642,
-0.021497169509530067,
-0.018811311572790146,
-0.007857154123485088,
-0.003863648045808077,
0.01383125502616167,
-0.00778285413980484,
0.02757156640291214,
0.0976189523935318,
0.04923615604639053,
-0.004517540335655212,
0.058846525847911835,
0.016964560374617577,
-0.08112925291061401,
-0.03883490711450577,
0.04886583611369133,
-0.021399950608611107,
0.07138141244649887,
-0.048520129173994064,
0.01575532555580139,
-0.017348380759358406,
0.01663343608379364,
-0.04846742004156113,
0.0661754384636879,
-0.116463802754879,
-0.057115696370601654,
0.10894620418548584,
0.03968968614935875,
-0.003714253194630146,
-0.030080603435635567,
0.06929410248994827,
-0.06212865933775902,
0.021547339856624603,
-0.06220117211341858,
0.05438794940710068,
-0.028129475191235542,
0.048092156648635864,
0.020536115393042564,
-0.0015888356138020754,
-0.023498449474573135,
-0.045857954770326614,
-0.0730537697672844,
-0.041815079748630524,
-0.05025786533951759,
0.008092707954347134,
0.028825893998146057,
-0.003770381910726428,
-0.04999687895178795,
-0.010885442607104778,
0.03008178249001503,
0.03965141624212265,
0.022490909323096275,
-0.01084714476019144,
0.047233037650585175,
-0.0335795022547245,
0.006916876416653395,
-0.00686060544103384,
0.05518645793199539,
-0.02166021056473255,
0.01676987297832966,
-0.04623972252011299,
0.0400630347430706,
0.023364407941699028,
0.06373575329780579,
-0.015431261621415615,
-0.012135283090174198,
0.023296788334846497,
-0.03437613695859909,
-0.024137383326888084,
-0.03075544908642769,
0.06910867244005203,
-0.024799318984150887,
-0.01546361856162548,
-0.06619783490896225,
0.014433799311518669,
-0.04999437555670738,
-0.006553007289767265,
-0.025266511365771294,
-0.010529601015150547,
-0.09436599910259247,
0.05156197026371956,
0.03526074439287186,
0.006305824499577284,
-0.00862424261868,
0.059992849826812744,
0.07044616341590881,
0.09111210703849792,
-0.04312621057033539,
-0.008742480538785458,
0.05179227888584137,
0.001677913125604391,
0.0406406931579113,
0.01405787281692028,
0.05272822454571724,
-0.015950683504343033,
-0.054161202162504196,
-0.015846705064177513,
-0.016085291281342506,
-0.018364107236266136,
0.05021330341696739,
0.00872397143393755,
0.03467433899641037,
0.018619021400809288,
0.03141225129365921,
-0.30081087350845337,
-0.03116328828036785,
0.0008719100733287632,
-0.010730825364589691,
0.018662575632333755,
-0.005755943711847067,
0.05164396017789841,
-0.055339764803647995,
-0.019159268587827682,
-0.05703958868980408,
0.012606736272573471,
0.08339012414216995,
-0.004163804464042187,
0.009730941615998745,
0.010082188993692398,
0.08233201503753662,
0.005044999066740274,
-0.052831847220659256,
-0.007092807907611132,
0.032019224017858505,
0.018589694052934647,
-0.006386354099959135,
-0.023380668833851814,
0.02776503376662731,
0.032143399119377136,
-0.021756412461400032,
0.0495549738407135,
-0.03872495889663696,
-0.023997029289603233,
0.012569534592330456,
0.00557932211086154,
0.02850276790559292,
-0.021463828161358833,
-0.11712886393070221,
0.000182786287041381,
-0.023676155135035515,
0.0673087015748024,
-0.004451318643987179,
-0.0184127576649189,
0.00891940575093031,
-0.02667824923992157,
0.03889142721891403,
0.006253253202885389,
0.010076243430376053,
-0.060680992901325226,
0.013505942188203335,
0.027577966451644897,
0.06900477409362793,
-0.053554926067590714,
-0.012026585638523102,
0.023486554622650146,
-0.018314825370907784,
0.02516648918390274,
-0.011230775155127048,
0.12255287170410156,
-0.0039476435631513596,
-0.07249624282121658,
-0.0549614243209362,
-0.02664826065301895,
-0.01819692738354206,
0.014520682394504547,
-0.0031778246629983187,
0.023871561512351036,
-0.031241366639733315,
-0.019367411732673645,
0.0786948949098587,
0.06747624278068542,
0.020030779764056206,
0.05002130940556526,
0.02080918289721012,
-0.01935010589659214,
0.10083850473165512,
-0.003493598196655512,
0.024363534525036812,
0.07456967234611511,
0.01092488132417202,
-0.07056169211864471,
-0.01845085434615612,
-0.005328869912773371,
0.016934657469391823,
0.0027350573800504208,
-0.053796302527189255,
0.013268854469060898,
0.041318465024232864,
-0.02227308228611946,
-0.03871186822652817,
0.024170322343707085,
0.04038047417998314,
0.014134097844362259,
0.006377173587679863,
-0.028798965737223625,
-0.009397614747285843,
-0.02279338799417019,
-0.05661128833889961,
-0.009582237340509892,
0.008656295947730541,
-0.2700020372867584,
-0.02271716110408306,
0.029943637549877167,
-0.037429746240377426,
-0.0452570766210556,
0.03441781923174858,
0.05626172199845314,
-0.02266957052052021,
-0.09166839718818665,
-0.01008780486881733,
-0.031375352293252945,
0.06313323229551315,
0.05081501230597496,
0.0595703050494194,
-0.08578769862651825,
-0.01729818806052208,
0.05441570654511452,
0.0007201075204648077,
0.027623608708381653,
-0.07284224778413773,
0.03809187933802605,
0.0045862155966460705,
0.12820954620838165,
-0.008999187499284744,
-0.03896366432309151,
0.04493771493434906,
0.011563481763005257,
0.025810685008764267,
-0.08081445097923279,
-0.012658119201660156,
-0.03199653699994087,
-0.00022811951930634677,
0.052675146609544754,
-0.025524234399199486,
-0.014295107685029507,
0.03954818844795227,
-0.034340210258960724,
0.011981290765106678,
-0.018578318879008293,
-0.027996260672807693,
-0.034067749977111816,
-0.010967112146317959,
-0.05797102302312851,
-0.04053608700633049,
0.07269179075956345,
-0.07522358745336533,
-0.07013693451881409,
0.014761198312044144,
-0.015501106157898903,
0.03622569888830185,
-0.0148807717487216,
-0.039409175515174866,
0.0546761192381382,
0.04170604795217514,
0.012327909469604492,
-0.06958667188882828,
-0.048356227576732635,
-0.0005228117224760354,
0.005145857110619545,
-0.00525252940133214,
-0.005066555459052324,
0.006941378116607666,
-0.026236634701490402,
0.0400032103061676,
0.050811517983675
] |
PMID:25018 | Sodium wasting, acidosis and hyperkalemia induced by methicillin interstitial nephritis. Evidence for selective distal tubular dysfunction. | A 61 year old male patient was studied who manifested dehydration, azotemia, acidosis and hyperkalemia six weeks after exposure to methicillin. Thyroid and adrenal glucocorticoid and mineralocorticoid function were normal. The dehydration was found to be caused by a profound sodium-losing nephropathy; urinary sodium ranged from 78 to 101 meq/day during a salt restricted diet. A distal renal tubular acidosis and a quantitively impaired ability to excrete potassium were also found. These defects were relatively unresponsive to mineralocorticoid or prednisone therapy. A renal biopsy specimen showed an interstitial nephritis which selectively affected distal tubules and was thought to be secondary to methicillin. The data suggest functional impairment specific for the distal tubule, but with only a modest decrease in the glomerular filtration rate. | Sodium wasting, acidosis and hyperkalemia induced by methicillin interstitial nephritis. Evidence for selective distal tubular dysfunction. A 61 year old male patient was studied who manifested dehydration, azotemia, acidosis and hyperkalemia six weeks after exposure to methicillin. Thyroid and adrenal glucocorticoid and mineralocorticoid function were normal. The dehydration was found to be caused by a profound sodium-losing nephropathy; urinary sodium ranged from 78 to 101 meq/day during a salt restricted diet. A distal renal tubular acidosis and a quantitively impaired ability to excrete potassium were also found. These defects were relatively unresponsive to mineralocorticoid or prednisone therapy. A renal biopsy specimen showed an interstitial nephritis which selectively affected distal tubules and was thought to be secondary to methicillin. The data suggest functional impairment specific for the distal tubule, but with only a modest decrease in the glomerular filtration rate. | [
-0.025000255554914474,
-0.01718316040933132,
0.06200351193547249,
0.05254827067255974,
0.005450667347759008,
-0.08584043383598328,
0.09010378271341324,
0.006979009136557579,
-0.045036230236291885,
-0.054802145808935165,
0.0029887782875448465,
-0.054044149816036224,
0.006653583142906427,
0.07131019234657288,
-0.03449937701225281,
-0.0043871537782251835,
0.06273353844881058,
0.0027405021246522665,
-0.08619160950183868,
-0.032497845590114594,
-0.016422294080257416,
0.005013595800846815,
0.02275201305747032,
-0.03779573366045952,
0.0239252969622612,
-0.011566044762730598,
0.03620900586247444,
0.037388961762189865,
-0.036678116768598557,
-0.16368769109249115,
0.016657788306474686,
0.008963722735643387,
-0.05220391973853111,
-0.0035118511877954006,
-0.07076697051525116,
0.0014724032953381538,
-0.054065871983766556,
0.002558703301474452,
0.00014687175280414522,
0.011081084609031677,
0.02453834004700184,
-0.008798183873295784,
0.02085266076028347,
0.05556965991854668,
-0.04439666494727135,
0.0038964299019426107,
-0.023899070918560028,
0.0040804133750498295,
0.17245130240917206,
0.05097761005163193,
0.003530643181875348,
-0.04231244698166847,
0.037422750145196915,
0.07986459136009216,
0.047737013548612595,
0.01644480787217617,
-0.03056635707616806,
0.04076867550611496,
0.005046363454312086,
0.010281060822308064,
0.014129438437521458,
0.00781826488673687,
-0.1442560851573944,
0.020220914855599403,
0.04841756448149681,
0.06132996082305908,
-0.04064396023750305,
-0.03335645794868469,
0.059097111225128174,
-0.012231232598423958,
0.0020290520042181015,
0.046336062252521515,
-0.03560960292816162,
0.04945947974920273,
0.002141272183507681,
-0.009873960167169571,
-0.0192565880715847,
-0.030762776732444763,
0.006427598651498556,
-0.006573578808456659,
0.025626078248023987,
0.0032569055911153555,
-0.0025739793200045824,
-0.024500280618667603,
0.013416530564427376,
-0.065648153424263,
0.007542360108345747,
-0.011653267778456211,
0.014703566208481789,
-0.04462407901883125,
-0.001795964315533638,
-0.012226726859807968,
0.010239961557090282,
0.02007504366338253,
-0.023807445541024208,
0.0620138980448246,
-0.022503068670630455,
-0.05528119578957558,
-0.06944604218006134,
0.39330410957336426,
0.005570846609771252,
0.028085285797715187,
-0.032942213118076324,
0.01065612118691206,
-0.010850414633750916,
-0.001167910173535347,
-0.0009858710691332817,
-0.035460442304611206,
-0.050072211772203445,
0.03713957220315933,
0.04031246155500412,
-0.029461819678544998,
0.026723535731434822,
-0.04349394515156746,
-0.033430855721235275,
0.005148821976035833,
0.0316368006169796,
-0.02567465230822563,
-0.005276774056255817,
0.059052325785160065,
-0.013865195214748383,
-0.035190604627132416,
0.017277643084526062,
-0.012148598209023476,
0.05858326703310013,
0.042068805545568466,
0.029922453686594963,
0.012556794099509716,
-0.013781789690256119,
0.009769362397491932,
0.06289376318454742,
0.024340765550732613,
-0.0007969144498929381,
0.01433408260345459,
-0.011375047266483307,
0.020896488800644875,
0.040447965264320374,
-0.08311795443296432,
0.02087269350886345,
0.05552082136273384,
-0.05490390211343765,
-0.06789117306470871,
-0.06261886656284332,
-0.06161404401063919,
-0.019169704988598824,
0.09428175538778305,
0.044830743223428726,
-0.006089213769882917,
0.0035171941854059696,
0.003950411919504404,
0.010012268088757992,
0.009432419203221798,
-0.03822556138038635,
0.02941393479704857,
0.012840671464800835,
-0.0023045947309583426,
-0.016992127522826195,
0.03789069503545761,
0.024388181045651436,
-0.030370401218533516,
-0.08113566786050797,
-0.07148236781358719,
0.0045024799183011055,
0.045570824295282364,
0.025059107691049576,
0.06111462041735649,
-0.0507633313536644,
-0.02144383080303669,
-0.03198819234967232,
0.06932628154754639,
-0.009451854974031448,
0.021101394668221474,
-0.016415003687143326,
-0.02897907979786396,
0.0031350364442914724,
0.008155634626746178,
0.03562219440937042,
0.0005383872194215655,
-0.03425535559654236,
-0.0671016201376915,
0.04221581295132637,
0.05470217764377594,
-0.013248235918581486,
0.025805899873375893,
0.0050987983122467995,
-0.03290112316608429,
-0.036526236683130264,
-0.014210464432835579,
0.0145864961668849,
0.014976457692682743,
-0.030497673898935318,
0.004711788613349199,
-0.07560314238071442,
-0.06644102185964584,
-0.03276592493057251,
-0.05118165537714958,
-0.007522380445152521,
0.030520504340529442,
-0.019995341077446938,
0.00480104424059391,
0.07773413509130478,
0.017621507868170738,
0.0340796560049057,
0.034102197736501694,
-0.04271992668509483,
0.0005200989544391632,
-0.02819664590060711,
-0.004275918938219547,
0.009914137423038483,
0.0008420628146268427,
-0.04302922263741493,
0.08401012420654297,
-0.0008833277388475835,
0.0036378337536007166,
-0.01602449081838131,
0.008362879976630211,
0.0024138381704688072,
-0.009519148617982864,
0.06237591803073883,
-0.008261206559836864,
0.08137012273073196,
-0.025592241436243057,
-0.05550999939441681,
-0.32995083928108215,
-0.0226142518222332,
0.046472951769828796,
-0.0042984639294445515,
0.07230529189109802,
-0.04499158635735512,
0.023049289360642433,
-0.02210848219692707,
-0.03543838858604431,
-0.026583297178149223,
0.023229945451021194,
0.08167924731969833,
0.0042580203153193,
-0.03691179305315018,
0.0214777123183012,
0.03977474942803383,
0.04117170348763466,
-0.043682754039764404,
0.06914598494768143,
0.014504552818834782,
0.011036507785320282,
-0.02469455637037754,
0.017922453582286835,
0.009776338003575802,
0.06803984194993973,
0.015996847301721573,
0.09813965111970901,
-0.008527815341949463,
-0.04363346844911575,
-0.1002008467912674,
0.0012299726950004697,
0.01982993260025978,
0.022569358348846436,
-0.04405597224831581,
-0.011625762097537518,
-0.02000925876200199,
-0.014776105061173439,
-0.015163024887442589,
-0.03349520266056061,
-0.0342659130692482,
-0.07898788154125214,
0.019575895741581917,
-0.03941209614276886,
-0.029510602355003357,
-0.024063320830464363,
0.01769290864467621,
-0.04402211681008339,
0.012427630834281445,
-0.013802110217511654,
-0.01187801267951727,
0.09660419821739197,
-0.005433008074760437,
-0.03806588798761368,
-0.010146131739020348,
0.04022415727376938,
-0.018182741478085518,
-0.05985911190509796,
-0.036894794553518295,
-0.052846018224954605,
-0.0444520078599453,
-0.019019730389118195,
-0.0002547190524637699,
0.05803942680358887,
-0.04423680901527405,
0.029649261385202408,
0.02771233394742012,
-0.02795048989355564,
0.032478783279657364,
0.04694418981671333,
0.028348030522465706,
-0.01756485551595688,
0.09474167972803116,
-0.02552652545273304,
0.00616650702431798,
0.0781298354268074,
0.04203435778617859,
-0.050771210342645645,
-0.05339052528142929,
-0.0337943360209465,
0.0462227538228035,
-0.0056620710529387,
-0.06111899018287659,
0.09078077971935272,
0.00403030589222908,
-0.008460178039968014,
0.09772714972496033,
-0.05725507438182831,
-0.03364315256476402,
0.018523123115301132,
-0.013003120198845863,
0.007472430355846882,
0.004320716951042414,
-0.03664363548159599,
-0.038888417184352875,
0.06533411145210266,
0.049835365265607834,
-0.24841462075710297,
0.03343664109706879,
0.02956245094537735,
-0.04316087067127228,
0.009158589877188206,
0.06418772786855698,
0.010830153711140156,
0.0012928784126415849,
-0.037153299897909164,
0.028428340330719948,
0.06588228791952133,
0.006405722349882126,
0.022425087168812752,
-0.02511514164507389,
0.04028769209980965,
0.0011645345948636532,
0.055525053292512894,
-0.04193345829844475,
0.004559563007205725,
-0.023387260735034943,
0.04462285339832306,
-0.02262650988996029,
0.0981210246682167,
-0.0035205718595534563,
-0.0671783983707428,
0.08715151250362396,
-0.008065113797783852,
0.06747463345527649,
-0.06482914835214615,
-0.014550719410181046,
-0.009468615986406803,
0.07035131007432938,
-0.0011408324353396893,
0.03957519307732582,
0.0438239611685276,
0.007489833049476147,
-0.05577077716588974,
0.045732803642749786,
0.021360933780670166,
0.016515644267201424,
0.004957336466759443,
-0.0067860702984035015,
-0.04931581765413284,
-0.03666027635335922,
0.04034186899662018,
-0.015913117676973343,
-0.0458090640604496,
-0.020872626453638077,
0.08528850227594376,
-0.0027421426493674517,
-0.04293930530548096,
-0.056184638291597366,
0.0689065083861351,
0.05586190149188042,
0.033067114651203156,
-0.019495511427521706,
0.020611314103007317,
-0.052883703261613846,
0.045658692717552185,
-0.059516701847314835,
0.002689346903935075,
0.005144438240677118,
-0.06740927696228027,
0.01688321866095066,
0.008216594345867634
] |
PMID:25021 | New concepts in dysmenorrhea. | The etiology of primary dysmenorrhea, which is the most common gynecologic complaint and cause of lost working hours, remains obscure but merits careful scientific investigation. Recent studies suggest that increased endometrial prostaglandin production and release may be responsible for dysmenorrhea. Prostaglandins cause myometrial contractility that, if excessive, leads to uterine ischemia and pain. This hypothesis has led to clinical trials of antiprostaglandin agents such as indomethacin and fenamates, which inhibit the synthesis of prostaglandin through the prostaglandin synthetase system as well as antagonize their action at the cell receptor level. The good response of dysmenorrhea to other conventional forms of therapy such as oral contraceptives and dilatation of the cervix can be partly explained on the basis of a reduced level of prostaglandins in the menstrual fluid with such therapy. There is a definite need for further evaluation of the antiprostaglandin compounds in the treatment of dysmenorrhea so that sound formulations can be evolved for the elimination of this incapacitating disorder. | New concepts in dysmenorrhea. The etiology of primary dysmenorrhea, which is the most common gynecologic complaint and cause of lost working hours, remains obscure but merits careful scientific investigation. Recent studies suggest that increased endometrial prostaglandin production and release may be responsible for dysmenorrhea. Prostaglandins cause myometrial contractility that, if excessive, leads to uterine ischemia and pain. This hypothesis has led to clinical trials of antiprostaglandin agents such as indomethacin and fenamates, which inhibit the synthesis of prostaglandin through the prostaglandin synthetase system as well as antagonize their action at the cell receptor level. The good response of dysmenorrhea to other conventional forms of therapy such as oral contraceptives and dilatation of the cervix can be partly explained on the basis of a reduced level of prostaglandins in the menstrual fluid with such therapy. There is a definite need for further evaluation of the antiprostaglandin compounds in the treatment of dysmenorrhea so that sound formulations can be evolved for the elimination of this incapacitating disorder. | [
0.0069294581189751625,
0.04343782365322113,
-0.032255981117486954,
0.04429183527827263,
0.008029874414205551,
0.0023744874633848667,
-0.02371327392756939,
-0.0005157182458788157,
-0.005774693097919226,
-0.06528622657060623,
0.0397489108145237,
-0.0430447980761528,
0.05638190731406212,
0.014384330250322819,
-0.051427844911813736,
-0.010509414598345757,
0.03196484223008156,
-0.0003343176213093102,
-0.07432655990123749,
-0.004393935203552246,
0.012568065896630287,
0.012524109333753586,
0.00045790529111400247,
0.007378905545920134,
0.057209715247154236,
0.008056895807385445,
0.006897512823343277,
0.017473388463258743,
-0.06131661310791969,
-0.1418841928243637,
0.02212958224117756,
0.00205922219902277,
0.025617850944399834,
0.018313664942979813,
0.013274663127958775,
-0.047007568180561066,
-0.06385877728462219,
0.019934767857193947,
0.006409758236259222,
0.03358142077922821,
0.0569668784737587,
-0.010651733726263046,
-0.00938638485968113,
-0.0058721317909657955,
0.049998365342617035,
0.07662162184715271,
0.002382674952968955,
-0.004802303854376078,
0.0949210450053215,
0.005323801655322313,
0.08774518221616745,
-0.006782140117138624,
-0.020087840035557747,
0.04075225070118904,
-0.0018905398901551962,
-0.08732452243566513,
-0.010961871594190598,
0.013669787906110287,
0.012676230631768703,
0.035360388457775116,
0.032273318618535995,
0.025340106338262558,
-0.1292760819196701,
0.04451364651322365,
0.01580715924501419,
0.00357795599848032,
0.03888748586177826,
-0.011001279577612877,
0.04804706200957298,
0.060241423547267914,
-0.08201774209737778,
0.05688713490962982,
-0.04929298907518387,
0.02460058033466339,
-0.0012728954898193479,
-0.001389126991853118,
0.012815040536224842,
-0.034559525549411774,
0.05656503885984421,
0.0804651752114296,
0.049787282943725586,
0.03914157301187515,
-0.0012422135332599282,
0.00014967721654102206,
-0.06123225763440132,
-0.09333637356758118,
0.0009666190599091351,
-0.011611076071858406,
0.011426691897213459,
-0.013165071606636047,
-0.03157118707895279,
-0.06341023743152618,
-0.025017373263835907,
-0.019286619499325752,
-0.027835024520754814,
0.026721475645899773,
-0.003494568169116974,
0.052834391593933105,
-0.05855882167816162,
0.3696233332157135,
-0.054526347666978836,
-0.027726560831069946,
-0.05900105461478233,
0.0022245130967348814,
0.025204934179782867,
-0.03704595938324928,
-0.006261289119720459,
0.01036750990897417,
-0.056710269302129745,
0.03301544487476349,
0.027342170476913452,
0.0558897964656353,
0.04343544691801071,
-0.03587701916694641,
0.011775520630180836,
-0.08055976778268814,
0.03695350140333176,
-0.03050360269844532,
-0.08239654451608658,
0.019721413031220436,
-0.0327620729804039,
-0.00999200064688921,
0.018909787759184837,
-0.020052632316946983,
0.04223775118589401,
0.0038607902824878693,
-0.013018053025007248,
0.061489347368478775,
0.017536750063300133,
-0.06385490298271179,
0.03806198015809059,
0.04943779855966568,
-0.058996666222810745,
0.03347458317875862,
0.0381169430911541,
-0.05436859652400017,
0.046737805008888245,
-0.012006986886262894,
0.02519523724913597,
-0.0062068612314760685,
-0.054472580552101135,
-0.03969259560108185,
-0.048285260796546936,
-0.03790882229804993,
0.0013981194933876395,
0.10734961181879044,
0.03322816640138626,
-0.03746642917394638,
0.014237239956855774,
0.021632060408592224,
0.02347266860306263,
0.0561937615275383,
-0.060680050402879715,
0.058318737894296646,
0.044420596212148666,
0.010951641947031021,
0.03463245928287506,
-0.06079290434718132,
-0.0020636855624616146,
-0.02927142009139061,
-0.02088392712175846,
0.03138335794210434,
-0.03884556144475937,
0.015266054309904575,
0.04625295475125313,
-0.003317595459520817,
-0.01589120179414749,
-0.0001424702350050211,
-0.032101064920425415,
-0.017822537571191788,
-0.05125240236520767,
-0.008025791496038437,
-0.060335006564855576,
-0.06874871253967285,
0.006655634380877018,
0.0008144174935296178,
0.0009531493415124714,
-0.008067321963608265,
0.0586550347507,
0.00420313561335206,
0.09510919451713562,
0.04465627297759056,
0.025645218789577484,
-0.02933315932750702,
0.018307145684957504,
-0.044656869024038315,
-0.0744645968079567,
0.003538849065080285,
-0.013646122999489307,
-0.04173559695482254,
0.04278649017214775,
-0.016658959910273552,
-0.06622720509767532,
0.000549808144569397,
-0.04907714203000069,
-0.030094169080257416,
-0.017109587788581848,
0.03987376391887665,
-0.043236613273620605,
-0.00024829135509207845,
0.06997833400964737,
0.02829943597316742,
0.052201852202415466,
0.05978785827755928,
0.04130939766764641,
0.033295560628175735,
0.01919962465763092,
-0.0019253755453974009,
0.010218022391200066,
-0.011895916424691677,
0.03403908386826515,
0.02818514220416546,
0.033317986875772476,
-0.02537176012992859,
-0.08618462085723877,
0.06126523017883301,
-0.008200319483876228,
-0.007491419557482004,
0.0650307685136795,
-0.03321688622236252,
0.05783066526055336,
0.05982385203242302,
0.04993075877428055,
-0.3096809387207031,
-0.1065680980682373,
-0.02176884189248085,
-0.05300549045205116,
0.05607134476304054,
0.041152987629175186,
-0.0325082503259182,
-0.05382037162780762,
0.008142795413732529,
-0.0178020391613245,
0.051123328506946564,
0.05939970910549164,
-0.06233398616313934,
-0.058508116751909256,
0.013992109335958958,
0.006406134460121393,
-0.012000410817563534,
-0.0286130141466856,
0.01904919184744358,
-0.025213085114955902,
-0.015614003874361515,
0.0019124463433399796,
-0.005711168982088566,
0.04024672880768776,
-0.0009200368658639491,
-0.023114468902349472,
0.08269251883029938,
-0.04666716605424881,
-0.017203105613589287,
-0.02960757352411747,
0.044319137930870056,
0.03207997605204582,
0.010015121661126614,
-0.10275662690401077,
-0.004279077518731356,
-0.039102792739868164,
0.05689554288983345,
-0.07053116708993912,
-0.0010779707226902246,
-0.035168539732694626,
-0.03550462797284126,
0.012449920177459717,
-0.0038798467721790075,
0.03497758135199547,
-0.08767791092395782,
0.009883047081530094,
-0.03232712298631668,
-0.005705258343368769,
0.0003415639803279191,
0.04027712717652321,
-0.014754190109670162,
-0.0036567228380590677,
0.03503766655921936,
-0.0020317621529102325,
-0.023702986538410187,
-0.023226195946335793,
-0.06474731862545013,
0.003276947420090437,
-0.06374908238649368,
-0.03279227018356323,
0.02501031756401062,
0.029764581471681595,
0.06107960641384125,
-0.06556257605552673,
-0.01948494277894497,
-0.016506358981132507,
0.006604653317481279,
-0.04190412536263466,
0.04859969764947891,
0.0578218474984169,
0.09103064239025116,
0.13798420131206512,
-0.09980102628469467,
0.03647387772798538,
0.09718117862939835,
0.04454812407493591,
0.004071887116879225,
-0.006097311154007912,
-0.08824777603149414,
-0.019258949905633926,
0.0354120135307312,
-0.07036706060171127,
0.027915755286812782,
0.03367190435528755,
0.014613629318773746,
0.001485170447267592,
-0.04222049191594124,
0.029012830927968025,
0.034703951328992844,
0.009206874296069145,
0.040513474494218826,
0.022621167823672295,
-0.015488171018660069,
-0.006437930278480053,
0.02934965491294861,
0.049771759659051895,
-0.2397255003452301,
-0.018035154789686203,
-0.004567351657897234,
-0.06670447438955307,
-0.0807749330997467,
0.0818287655711174,
-0.0695360004901886,
-0.013646700419485569,
-0.03404886648058891,
0.0073733264580369,
0.024799777194857597,
0.006416317541152239,
0.012671632692217827,
0.0014810699503868818,
-0.0035271779634058475,
0.020007017999887466,
0.07542772591114044,
0.06123080849647522,
0.03762764483690262,
-0.04419237747788429,
-0.006912548094987869,
-0.042911894619464874,
0.1099710613489151,
0.012081412598490715,
-0.022675679996609688,
0.05400735139846802,
0.0546705462038517,
0.02728685550391674,
-0.029062146320939064,
0.08134927600622177,
-0.008075418882071972,
0.05982404202222824,
0.05866532027721405,
-0.021168023347854614,
-0.03584153950214386,
0.02249756082892418,
-0.030596941709518433,
-0.010954860597848892,
0.03536023944616318,
-0.004970666952431202,
-0.020677175372838974,
-0.04959685355424881,
-0.0649944469332695,
0.008102026768028736,
0.0512753464281559,
-0.044518303126096725,
-0.0570085383951664,
-0.04233376309275627,
0.0379941388964653,
0.032288145273923874,
-0.014970172196626663,
-0.07298135757446289,
0.024305419996380806,
0.049548134207725525,
0.04592745378613472,
-0.02654317207634449,
0.031042005866765976,
-0.00865129567682743,
0.014248605817556381,
-0.007467343006283045,
0.0028657803777605295,
-0.06350782513618469,
-0.030192727223038673,
0.054747793823480606,
0.0001616402150830254
] |
PMID:25022 | Subtle sequelae of brain damage. Perplexity, distractibility, and fatigue. | Subtle problems of perplexity, distractibility, and fatigue accompany all kinds of brain injury for they appear to result from disruption of accustomed neural pathways and loss or change of mental function. Emotional disturbances may mask these subtle problems, but they can also result from them. Clinical experience indicates that these problems are more apt to become stressful when the patient misinterprets or copes ineffectively with them. That these common problems of brain injured adults may be overlooked in the usual clinical examination was shown in a comparison of clinical records of 50 patients referred for neuropsychological consultation with records of 46 patient-participants in a longitudinal neuropsychological study. Significantly more reports of these problems appeared in the latter group of records. However, consultation records did reflect emotional distress. Irritability, depression, or anxiety affected all but six consultation patients and appeared with equal frequency among working patients as among those unable to work or needing full-time care. This suggests that some of their emotional distress resulted from less obvious problems than those impairing mobility, strength, or competency. Counseling can reduce the patient's vulnerability to the psychologically crippling effects of perplexity, distractibility, and fatigue. Specific recommendations for patient and family counseling are offered. | Subtle sequelae of brain damage. Perplexity, distractibility, and fatigue. Subtle problems of perplexity, distractibility, and fatigue accompany all kinds of brain injury for they appear to result from disruption of accustomed neural pathways and loss or change of mental function. Emotional disturbances may mask these subtle problems, but they can also result from them. Clinical experience indicates that these problems are more apt to become stressful when the patient misinterprets or copes ineffectively with them. That these common problems of brain injured adults may be overlooked in the usual clinical examination was shown in a comparison of clinical records of 50 patients referred for neuropsychological consultation with records of 46 patient-participants in a longitudinal neuropsychological study. Significantly more reports of these problems appeared in the latter group of records. However, consultation records did reflect emotional distress. Irritability, depression, or anxiety affected all but six consultation patients and appeared with equal frequency among working patients as among those unable to work or needing full-time care. This suggests that some of their emotional distress resulted from less obvious problems than those impairing mobility, strength, or competency. Counseling can reduce the patient's vulnerability to the psychologically crippling effects of perplexity, distractibility, and fatigue. Specific recommendations for patient and family counseling are offered. | [
-0.01386573351919651,
0.03689108416438103,
0.0020596375688910484,
0.028947990387678146,
-0.03344004228711128,
0.014117255806922913,
0.07923019677400589,
0.03664620593190193,
0.012508349493145943,
-0.06500594317913055,
0.015274477191269398,
0.0031113699078559875,
0.02443801984190941,
0.061848290264606476,
-0.004370905924588442,
0.06012376397848129,
0.001336916466243565,
0.018108496442437172,
-0.04374845698475838,
0.055518943816423416,
-0.07778380811214447,
0.01742035709321499,
0.04069622978568077,
0.0524895153939724,
-0.0215449221432209,
0.0321660190820694,
-0.03358040377497673,
0.012590707279741764,
-0.04119165614247322,
-0.15178894996643066,
0.022022902965545654,
0.014662413857877254,
-0.006679842714220285,
-0.02921454794704914,
0.019949477165937424,
0.02311547100543976,
-0.03605101630091667,
0.05840631201863289,
-0.0029843850061297417,
-0.0323539599776268,
0.03610406443476677,
0.020894808694720268,
-0.023763353005051613,
-0.04258561134338379,
-0.021506819874048233,
-0.009978837333619595,
0.002655050717294216,
-0.02156686969101429,
0.09670819342136383,
-0.02061205357313156,
-0.056836310774087906,
-0.071857750415802,
0.039026614278554916,
0.05134734883904457,
-0.002271228702738881,
-0.008089162409305573,
0.05613821744918823,
0.020883353427052498,
-0.013862734660506248,
0.07026132196187973,
-0.013112025335431099,
0.026546752080321312,
-0.07410413026809692,
0.01806461811065674,
0.05824338272213936,
-0.02669142745435238,
-0.028835918754339218,
-0.10447461903095245,
0.013901646248996258,
0.040968313813209534,
-0.05214225873351097,
0.04245004802942276,
-0.00634734658524394,
0.06143321841955185,
0.05218945071101189,
-0.05154777690768242,
-0.018860189244151115,
-0.03639509528875351,
-0.0028911042027175426,
0.03131715953350067,
0.03972398489713669,
0.008938804268836975,
-0.034621257334947586,
-0.04091605544090271,
0.004656633362174034,
-0.012934661470353603,
-0.0017947969026863575,
-0.045807287096977234,
-0.025941528379917145,
-0.01927330531179905,
-0.047513198107481,
-0.00913213286548853,
-0.008008276112377644,
0.03412410616874695,
-0.029830945655703545,
-0.026464663445949554,
0.005610368680208921,
-0.03465438261628151,
-0.06427035480737686,
0.4060472846031189,
-0.0007129498990252614,
0.02049710974097252,
0.04952166974544525,
0.014509784989058971,
-0.03464218229055405,
-0.0469675287604332,
0.026889001950621605,
-0.05734479799866676,
-0.01907469891011715,
0.046876974403858185,
0.0186553206294775,
-0.023900510743260384,
0.01899944432079792,
-0.013655388727784157,
-0.006883395370095968,
0.06254113465547562,
0.07193931937217712,
-0.014112011529505253,
0.01055432204157114,
0.009334909729659557,
-0.0009740058449096978,
-0.01857585459947586,
0.031666211783885956,
-0.018368659541010857,
-0.03310959413647652,
-0.019916128367185593,
0.01838095486164093,
0.06852394342422485,
0.05155971646308899,
-0.09591202437877655,
0.046254221349954605,
0.000053804593335371464,
0.05019516125321388,
0.041909825056791306,
0.016866769641637802,
-0.007128579076379538,
-0.00437980517745018,
-0.037234846502542496,
0.010719877667725086,
0.027467316016554832,
0.02559853345155716,
-0.030617207288742065,
0.004288462921977043,
-0.06507325172424316,
-0.08908811956644058,
0.1125451996922493,
-0.020181752741336823,
-0.017418432980775833,
-0.025863388553261757,
-0.01773091033101082,
-0.03364335745573044,
-0.01693612150847912,
-0.017080038785934448,
0.04821455851197243,
-0.008726265281438828,
-0.00013346409832593054,
0.029986001551151276,
-0.018890874460339546,
-0.012671181000769138,
0.04856516420841217,
0.07771611958742142,
-0.04116930812597275,
-0.015523390844464302,
0.09961756318807602,
0.0191443283110857,
0.04290584847331047,
-0.0038327996153384447,
-0.03298230841755867,
-0.021548213437199593,
-0.049210432916879654,
0.015200178138911724,
-0.011504546739161015,
0.027606278657913208,
-0.025562625378370285,
0.0337679460644722,
0.021537456661462784,
-0.05116566643118858,
0.015445037744939327,
-0.024963902309536934,
0.02814001590013504,
0.07542221993207932,
-0.015038671903312206,
-0.02883070521056652,
0.021777868270874023,
-0.008196241222321987,
-0.046544477343559265,
0.03444971889257431,
0.0373246967792511,
0.050195563584566116,
-0.03487647324800491,
-0.048150233924388885,
-0.06442337483167648,
-0.05339017137885094,
0.02024921029806137,
-0.03134208917617798,
0.004596605431288481,
-0.03089298866689205,
-0.04633912071585655,
-0.0495535172522068,
0.03226077929139137,
0.018406467512249947,
-0.00892779789865017,
0.0020169138442724943,
0.047487761825323105,
0.05814509838819504,
0.006951814983040094,
0.010961601510643959,
-0.03642675280570984,
0.04892279952764511,
0.051378972828388214,
0.0013884229119867086,
0.0015165634686127305,
-0.011964810080826283,
0.028612688183784485,
0.017699670046567917,
0.04549665376543999,
0.010907791554927826,
0.027168670669198036,
0.06462886929512024,
0.039214372634887695,
0.047183647751808167,
-0.031077217310667038,
-0.03642677888274193,
-0.31300246715545654,
-0.06401731073856354,
-0.005003468133509159,
-0.039759229868650436,
-0.013560113497078419,
-0.05655890703201294,
-0.0234559066593647,
0.009655087254941463,
0.003953771665692329,
0.005479215178638697,
-0.0019526878604665399,
0.03696586936712265,
-0.09862343966960907,
-0.06432917714118958,
0.03729065880179405,
-0.006859452463686466,
0.010628797113895416,
-0.05936381593346596,
-0.02994544990360737,
-0.05366775766015053,
0.010086520574986935,
0.06251399964094162,
0.046585097908973694,
-0.05055234208703041,
-0.03138504922389984,
0.011613971553742886,
0.12705165147781372,
-0.021754775196313858,
0.01363067701458931,
-0.030395187437534332,
-0.0784679725766182,
0.006331328768283129,
-0.03248996287584305,
-0.08325853198766708,
0.061459653079509735,
-0.027160892263054848,
0.00940538477152586,
-0.015006623230874538,
-0.06352418661117554,
-0.027475951239466667,
-0.08431588858366013,
0.0015380254480987787,
-0.007238506339490414,
0.006422146689146757,
-0.09730443358421326,
-0.018324565142393112,
0.003277409356087446,
-0.017622536048293114,
-0.0049706134013831615,
-0.0018267880659550428,
0.031718380749225616,
-0.006464455742388964,
-0.006668641697615385,
0.01788191869854927,
0.08299674093723297,
-0.013678259216248989,
-0.096348837018013,
0.029824763536453247,
-0.03117372654378414,
0.006937641184777021,
-0.0159642044454813,
-0.009101335890591145,
0.06760436296463013,
-0.049812864512205124,
0.004239812958985567,
0.06482767313718796,
0.013241319917142391,
-0.037157364189624786,
0.011235469952225685,
0.047232139855623245,
0.010395186021924019,
0.1315746158361435,
-0.008457021787762642,
-0.018392091616988182,
0.01902955211699009,
0.008692240342497826,
-0.015557626262307167,
-0.0018167172092944384,
-0.03395181521773338,
0.014131815172731876,
0.05358382686972618,
0.020814893767237663,
-0.0035481443628668785,
0.06181322783231735,
-0.0019090869463980198,
-0.04529457539319992,
-0.027939504012465477,
-0.05439861863851547,
0.03014044277369976,
-0.0663156807422638,
-0.010174953378736973,
0.02371869795024395,
-0.030951103195548058,
-0.06288366764783859,
0.06179354712367058,
0.006868663243949413,
-0.29728278517723083,
0.028016364201903343,
-0.027581827715039253,
0.019649075344204903,
0.02077106386423111,
0.05336572974920273,
-0.05512319505214691,
0.049020543694496155,
-0.023823251947760582,
-0.029062196612358093,
0.037795290350914,
0.00909463595598936,
0.03045288473367691,
-0.04301350191235542,
-0.03991512209177017,
0.05752179026603699,
0.06586436927318573,
-0.009794794954359531,
0.03620055317878723,
0.037447042763233185,
0.015400188975036144,
0.04530149698257446,
0.16173459589481354,
-0.0013627742882817984,
0.028328338637948036,
-0.006059625186026096,
0.05526838079094887,
0.035958629101514816,
-0.006017429754137993,
-0.0020622359588742256,
0.03610416129231453,
-0.013772659003734589,
0.006512554362416267,
0.011355589143931866,
0.03720468282699585,
-0.032597851008176804,
-0.05257420241832733,
0.011460312642157078,
0.02555716596543789,
-0.06014057621359825,
0.020666038617491722,
-0.026986608281731606,
-0.0011613252572715282,
-0.041369207203388214,
0.08284369856119156,
0.030076272785663605,
-0.006888215430080891,
-0.05019531771540642,
-0.016481908038258553,
-0.04254691302776337,
-0.07183592021465302,
-0.0147256376221776,
0.062054313719272614,
0.014758474193513393,
0.042986318469047546,
-0.008104742504656315,
0.03315204381942749,
0.012068521231412888,
0.06698774546384811,
-0.05034329742193222,
-0.023830700665712357,
0.03219630569219589,
0.0030228886753320694,
0.032252974808216095,
0.007149758283048868
] |
PMID:25019 | The syndrome of post-splenectomy fulminant sepsis. Case report and review of the literature. | Three years after splenectomy, a middle-aged woman had two separate episodes of fulminant bacterial sepsis. She recovered each time with prompt and appropriate treatment. Her immunologic system was examined and found to be normal with respect to antibody formation against diphtheria and tetanus toxoid, granulocyte killing of staphylococci and serum opsonizing activity. Granulocytes, bursa-equivalent (B) and thymus-dependent (T) lymphocytes and serum immunoglobulins were quantitatively normal. She produced antibody against subcutaneously injected polyvalent pneumococcal vaccine. She demonstrated cutaneous anergy. The literature on this syndrome was reviewed in an attempt to ascertain why hyposplenic patients are subject of fulminant bacterial, chiefly pneumococcal, sepsis. The probably explanation is the delayed production of antibodies against the phagocytic-resistant capsule of certain bacteria which the host had not previously encountered. The incidence of this syndrome appears to be on the order of 0.5 to 1.0%/year for splenectomized older children and adults. As the syndrome is seen chiefly in splenectomized or othewise hyposplenic patients, a causal relationship seems to exist. | The syndrome of post-splenectomy fulminant sepsis. Case report and review of the literature. Three years after splenectomy, a middle-aged woman had two separate episodes of fulminant bacterial sepsis. She recovered each time with prompt and appropriate treatment. Her immunologic system was examined and found to be normal with respect to antibody formation against diphtheria and tetanus toxoid, granulocyte killing of staphylococci and serum opsonizing activity. Granulocytes, bursa-equivalent (B) and thymus-dependent (T) lymphocytes and serum immunoglobulins were quantitatively normal. She produced antibody against subcutaneously injected polyvalent pneumococcal vaccine. She demonstrated cutaneous anergy. The literature on this syndrome was reviewed in an attempt to ascertain why hyposplenic patients are subject of fulminant bacterial, chiefly pneumococcal, sepsis. The probably explanation is the delayed production of antibodies against the phagocytic-resistant capsule of certain bacteria which the host had not previously encountered. The incidence of this syndrome appears to be on the order of 0.5 to 1.0%/year for splenectomized older children and adults. As the syndrome is seen chiefly in splenectomized or othewise hyposplenic patients, a causal relationship seems to exist. | [
-0.02816520258784294,
0.048215970396995544,
-0.04541612043976784,
0.0020184635650366545,
0.002558052772656083,
-0.013564332388341427,
0.07247871905565262,
0.11754457652568817,
-0.033693354576826096,
-0.03866448998451233,
0.0014607307966798544,
-0.04766831547021866,
-0.008597802370786667,
-0.008507899940013885,
-0.0410025380551815,
-0.01529926247894764,
0.032357845455408096,
0.018497155979275703,
-0.09084588289260864,
0.02941144071519375,
-0.07016948610544205,
0.046983495354652405,
-0.03070867620408535,
-0.015945864841341972,
0.03306267410516739,
0.011438840068876743,
0.011985043995082378,
-0.045371007174253464,
-0.044655732810497284,
-0.1688733547925949,
-0.03257745876908302,
-0.05365367978811264,
0.005512189585715532,
-0.009491745382547379,
0.020668527111411095,
-0.009477583691477776,
0.010150440968573093,
-0.013466522097587585,
0.001366997486911714,
0.05133841559290886,
-0.0169044341892004,
0.038217637687921524,
0.0421406626701355,
-0.02513389103114605,
0.03606787323951721,
0.0012170393019914627,
-0.030259139835834503,
0.027823135256767273,
0.12273985892534256,
0.013534219935536385,
-0.01201064232736826,
0.014497701078653336,
0.013449191115796566,
0.1147298663854599,
0.01196729950606823,
-0.043750811368227005,
-0.03260225057601929,
0.020252779126167297,
-0.03785603865981102,
0.011729257181286812,
-0.013247647322714329,
-0.01839740201830864,
-0.08945111930370331,
0.0565253309905529,
0.06066069006919861,
-0.03839539363980293,
0.025610027834773064,
-0.054786957800388336,
0.0733240619301796,
0.018118856474757195,
-0.009898480027914047,
0.03614159673452377,
-0.05033870041370392,
0.10502376407384872,
-0.0243393424898386,
-0.006847982294857502,
-0.006263624876737595,
-0.02741631120443344,
0.03255081549286842,
0.05263398587703705,
0.022428972646594048,
0.028251981362700462,
-0.020529022440314293,
0.008827713318169117,
-0.06467829644680023,
-0.0607442669570446,
-0.0015115025453269482,
-0.05334276705980301,
-0.012610341422259808,
-0.010029491037130356,
0.015237663872539997,
-0.04247310757637024,
0.0018636543536558747,
0.043771445751190186,
-0.05226457491517067,
-0.027896739542484283,
-0.06907884031534195,
-0.0016383283073082566,
0.04858582094311714,
0.45065072178840637,
-0.04984082281589508,
-0.03528085723519325,
0.008558724075555801,
0.0026434799656271935,
0.029256245121359825,
0.01434156484901905,
0.03870255500078201,
-0.006974516436457634,
-0.0005732112913392484,
0.015895511955022812,
0.004289445001631975,
0.0028379783034324646,
0.06496617943048477,
0.009201046079397202,
-0.022154241800308228,
0.03381772339344025,
0.06504098325967789,
0.055311840027570724,
-0.018133273348212242,
0.008151302114129066,
0.054088953882455826,
-0.03467266261577606,
0.0020808333065360785,
-0.08565619587898254,
0.02043103240430355,
0.033964455127716064,
0.02893137000501156,
0.08695460855960846,
-0.003051964333280921,
-0.00946605484932661,
0.07662342488765717,
0.029498377814888954,
0.007725497242063284,
-0.01688649132847786,
-0.004469160456210375,
0.023237626999616623,
0.03506177291274071,
0.004032752476632595,
0.013934887945652008,
-0.020477192476391792,
0.020404044538736343,
-0.07029029726982117,
-0.02666139043867588,
-0.06870929896831512,
-0.033318232744932175,
0.06479267030954361,
-0.04164542630314827,
0.025992540642619133,
-0.0396684966981411,
0.020124752074480057,
-0.035125214606523514,
0.07377795875072479,
-0.011617124080657959,
0.02527090162038803,
-0.006338173523545265,
0.016595466062426567,
-0.019457409158349037,
-0.000837388273794204,
0.01665719598531723,
0.032597217708826065,
-0.05104915425181389,
-0.039012521505355835,
0.002292497316375375,
0.04540880024433136,
0.01987438276410103,
0.010426182299852371,
-0.008947462774813175,
-0.028094593435525894,
-0.009915548376739025,
0.002886423608288169,
0.000376909039914608,
0.011288497596979141,
-0.023525407537817955,
-0.047716692090034485,
0.01520980428904295,
0.009889301843941212,
0.021383358165621758,
-0.021556371822953224,
-0.011156211607158184,
0.04821079596877098,
-0.02797034941613674,
0.04471522197127342,
0.02577054873108864,
0.015312910079956055,
-0.007631492335349321,
-0.044217582792043686,
-0.04436911270022392,
0.0585632249712944,
0.07148263603448868,
0.013686846941709518,
-0.011461544781923294,
-0.028223736211657524,
-0.05725199729204178,
-0.021906446665525436,
0.031632889062166214,
-0.03169524297118187,
-0.029393592849373817,
-0.004390343092381954,
0.044373154640197754,
-0.02471500262618065,
0.0984790250658989,
-0.00554443197324872,
-0.011054227128624916,
0.014903904870152473,
0.014536203816533089,
0.012152872048318386,
0.043363913893699646,
-0.011263309046626091,
0.053846217691898346,
-0.008406987413764,
-0.024083750322461128,
0.03730768337845802,
-0.007880207151174545,
0.0074579776264727116,
-0.014171548187732697,
0.07211413234472275,
-0.03641451895236969,
-0.020656678825616837,
0.03515460714697838,
-0.012856962159276009,
0.05882571265101433,
0.019219717010855675,
-0.021217355504631996,
-0.3158304691314697,
0.011515616439282894,
-0.022007545456290245,
-0.07049211114645004,
-0.03365996852517128,
0.040945976972579956,
-0.06632833182811737,
-0.012281805276870728,
0.003168074181303382,
-0.013055223971605301,
0.0948917418718338,
0.07199446856975555,
-0.04551276937127113,
-0.06493201851844788,
0.015449052676558495,
0.06591925024986267,
0.03714972734451294,
-0.08135217428207397,
0.006717565469443798,
0.014099914580583572,
-0.016984453424811363,
-0.021540462970733643,
0.013112583197653294,
0.030209165066480637,
0.007073312997817993,
-0.030496815219521523,
0.11421393603086472,
0.021929262205958366,
0.01953410543501377,
-0.02662310004234314,
-0.012462134473025799,
0.014434426091611385,
-0.009782858192920685,
-0.0311106089502573,
-0.020549336448311806,
0.0026352866552770138,
-0.00888361781835556,
-0.033201295882463455,
-0.018606439232826233,
0.012926376424729824,
-0.0867127850651741,
0.0007177212974056602,
-0.03894384577870369,
-0.018017882481217384,
-0.004595515783876181,
-0.007202791981399059,
0.027961168438196182,
0.03136245533823967,
-0.04828326404094696,
0.03576090559363365,
0.010537024587392807,
-0.05817129462957382,
-0.0389285646378994,
0.040367353707551956,
0.0046485899947583675,
-0.007319225464016199,
-0.04133279621601105,
0.003089817240834236,
-0.07204389572143555,
0.021273234859108925,
-0.004177382215857506,
-0.013004829175770283,
0.01876141130924225,
0.019017567858099937,
-0.03726363927125931,
0.01595272496342659,
0.026449836790561676,
-0.014953050762414932,
0.04696723073720932,
0.011068864725530148,
-0.0692119374871254,
0.12844812870025635,
-0.03508927300572395,
-0.03266352042555809,
0.010039974935352802,
0.07933155447244644,
0.000707874190993607,
-0.011311286129057407,
-0.038294896483421326,
-0.005013681948184967,
0.0848315879702568,
-0.06403861194849014,
0.02796410582959652,
0.05237273871898651,
-0.013242927379906178,
0.03675539791584015,
-0.053660038858652115,
0.004186603240668774,
-0.01637606881558895,
-0.018187187612056732,
-0.008150800131261349,
0.002721702679991722,
-0.0691046193242073,
-0.022694971412420273,
0.00006654927710769698,
-0.011823496781289577,
-0.25405153632164,
0.035383664071559906,
-0.0018631290877237916,
-0.039857614785432816,
0.0076519884169101715,
-0.011792588047683239,
-0.02300466038286686,
-0.041549425572156906,
-0.009319004602730274,
0.026524513959884644,
0.06534338742494583,
-0.015741096809506416,
0.040790461003780365,
0.0036393236368894577,
-0.04173393175005913,
0.0025684144347906113,
-0.03173854202032089,
0.028394555673003197,
0.01098268199712038,
0.017961330711841583,
0.005026299972087145,
-0.03245646134018898,
0.11612161248922348,
-0.0013011317932978272,
0.0046589067205786705,
-0.026666022837162018,
0.03661658987402916,
0.08729735016822815,
-0.04570601508021355,
0.05354519560933113,
0.07195527106523514,
-0.013535134494304657,
0.03822185471653938,
-0.0037241182290017605,
-0.0063498117960989475,
-0.03041108138859272,
-0.03144921734929085,
0.03774811327457428,
0.016767878085374832,
0.021467743441462517,
-0.07097926735877991,
-0.007115982007235289,
0.037865906953811646,
-0.06919234246015549,
0.10733398050069809,
-0.029706614091992378,
-0.036421049386262894,
-0.04913819208741188,
0.0035624406300485134,
0.017415696755051613,
-0.04193653166294098,
0.005632931366562843,
0.04542316868901253,
-0.0201284009963274,
0.03677442669868469,
-0.033012889325618744,
0.029162520542740822,
0.008298412896692753,
0.010236010886728764,
-0.014371426776051521,
-0.04796328768134117,
-0.07165484130382538,
-0.09040765464305878,
0.07683539390563965,
0.02543516270816326
] |
PMID:25026 | Smoking education programs 1960-1976. | This paper is a review of published reports, in English, of educational programs designed to change smoking behavior. Attempts to change the smoking behavior of young people have included anti-smoking campaigns, youth-to-youth programs, and a variety of message themes and teaching methods. Instruction has been presented both by teachers who were committed or persuasive and by teachers who were neutral or presented both sides of the issue. Didactic teaching, group discussion, individual study, peer instruction, and mass media have been employed. Health effects of smoking, both short- and long-term effects, have been emphasized. Most methods used with youth have shown little success. Studies of other methods have produced contradictory results. Educational programs for adults have included large scale anti-smoking campaigns, smoking cessation clinics, and a variety of more specific withdrawal methods. These methods have included individual counseling, emotional role playing, aversive conditioning, desensitization, and specific techniques to reduce the likelihood that smoking will occur in situations previously associated with smoking. Some of these techniques have produced poor results while studies of other methods have shown inconsistent results. The two methods showing the most promise are individual counseling and smoking withdrawal clinics. | Smoking education programs 1960-1976. This paper is a review of published reports, in English, of educational programs designed to change smoking behavior. Attempts to change the smoking behavior of young people have included anti-smoking campaigns, youth-to-youth programs, and a variety of message themes and teaching methods. Instruction has been presented both by teachers who were committed or persuasive and by teachers who were neutral or presented both sides of the issue. Didactic teaching, group discussion, individual study, peer instruction, and mass media have been employed. Health effects of smoking, both short- and long-term effects, have been emphasized. Most methods used with youth have shown little success. Studies of other methods have produced contradictory results. Educational programs for adults have included large scale anti-smoking campaigns, smoking cessation clinics, and a variety of more specific withdrawal methods. These methods have included individual counseling, emotional role playing, aversive conditioning, desensitization, and specific techniques to reduce the likelihood that smoking will occur in situations previously associated with smoking. Some of these techniques have produced poor results while studies of other methods have shown inconsistent results. The two methods showing the most promise are individual counseling and smoking withdrawal clinics. | [
0.013717756606638432,
0.06768728047609329,
0.04955645278096199,
0.0025945049710571766,
0.015738075599074364,
0.07005980610847473,
-0.03267138451337814,
0.020811231806874275,
-0.026560628786683083,
0.010051906108856201,
-0.004811233840882778,
-0.002150396117940545,
-0.025195976719260216,
0.012258063070476055,
-0.011598575860261917,
0.005186575930565596,
0.010636291466653347,
-0.0028525113593786955,
-0.017698490992188454,
0.0046661244705319405,
-0.018853826448321342,
0.04217031970620155,
-0.005893264431506395,
-0.005401975009590387,
0.08220086991786957,
0.029212646186351776,
-0.017288079485297203,
-0.039005205035209656,
-0.021437106654047966,
-0.08459913730621338,
0.08058418333530426,
-0.03900979831814766,
-0.0018129446543753147,
0.021388093009591103,
-0.049815695732831955,
0.013609785586595535,
-0.011947588995099068,
0.009108027443289757,
-0.05794065445661545,
0.05209740251302719,
0.010465597733855247,
0.03174780681729317,
-0.016282659024000168,
-0.0161821860820055,
-0.035772696137428284,
-0.008889948017895222,
-0.05439000576734543,
-0.07356199622154236,
0.05295608192682266,
-0.007941105403006077,
0.02378883771598339,
-0.03886764124035835,
0.033331722021102905,
0.0045058391988277435,
0.000569562369491905,
-0.03767231106758118,
-0.005609116982668638,
-0.0030431796330958605,
-0.0357939787209034,
0.04964466392993927,
-0.023988472297787666,
0.03768608346581459,
-0.22440597414970398,
0.09555120021104813,
-0.0023888065479695797,
0.06119203567504883,
0.002711967332288623,
0.005094015505164862,
0.10945018380880356,
0.017757175490260124,
-0.08547286689281464,
0.018794286996126175,
0.0037887683138251305,
0.06280849128961563,
0.03926270082592964,
0.005872477777302265,
-0.03297744691371918,
0.004352915100753307,
-0.0061465599574148655,
-0.03434327244758606,
0.004261216148734093,
0.018098536878824234,
0.04165612906217575,
-0.04206768795847893,
-0.0031161780934780836,
-0.051410309970378876,
0.024398166686296463,
-0.08880883455276489,
0.008539884351193905,
0.009183218702673912,
-0.006514958571642637,
0.039921004325151443,
-0.025120463222265244,
-0.022092510014772415,
-0.022728297859430313,
-0.041267022490501404,
0.02702190913259983,
-0.02481827884912491,
-0.061160724610090256,
0.4090527296066284,
-0.036028046160936356,
0.0025137346237897873,
-0.005213941913098097,
-0.008167184889316559,
0.0010311667574569583,
-0.044107601046562195,
-0.02706381119787693,
-0.07546533644199371,
-0.0068914745934307575,
0.023924918845295906,
0.006911757402122021,
-0.02090030163526535,
0.036214716732501984,
-0.006682836450636387,
-0.05862056091427803,
0.015580571256577969,
0.05218581482768059,
0.038994885981082916,
0.026733916252851486,
0.041430141776800156,
-0.0369592159986496,
0.036943551152944565,
0.0347878597676754,
-0.08363258838653564,
0.028990553691983223,
-0.08546668291091919,
-0.03707388415932655,
0.10373448580503464,
-0.004182765260338783,
-0.00007831653783796355,
0.07316281646490097,
0.004186585545539856,
-0.017725655809044838,
0.006688730791211128,
-0.04024579003453255,
-0.03174838423728943,
0.01009650994092226,
0.026091376319527626,
-0.008119132369756699,
0.016971305012702942,
0.057739049196243286,
-0.007076174486428499,
0.022353777661919594,
-0.085525743663311,
-0.04574788734316826,
0.06768961995840073,
0.05772580951452255,
0.03628268465399742,
-0.0039122868329286575,
0.054892051964998245,
0.04933995380997658,
0.018249111250042915,
-0.07824133336544037,
0.012170209549367428,
0.02472756803035736,
-0.009500850923359394,
0.07018162310123444,
0.021582771092653275,
0.03168207034468651,
-0.015210787765681744,
-0.018789784982800484,
-0.047902774065732956,
0.023002058267593384,
0.0483933761715889,
0.06499561667442322,
0.007986188866198063,
-0.045306410640478134,
-0.008424462750554085,
-0.017129333689808846,
-0.06752512603998184,
0.02051127515733242,
0.017465787008404732,
0.023609712719917297,
-0.013081394135951996,
-0.018543563783168793,
0.027711134403944016,
-0.0025287456810474396,
-0.011691727675497532,
0.00026442582020536065,
0.042351365089416504,
0.08116624504327774,
-0.021024391055107117,
0.03131664916872978,
0.004837804939597845,
0.01494191586971283,
0.00729181757196784,
0.00770384818315506,
-0.04188086837530136,
0.08063202351331711,
0.022220250219106674,
-0.037543706595897675,
-0.03732293099164963,
-0.08399016410112381,
-0.02232404239475727,
-0.005881379824131727,
-0.058998651802539825,
-0.010787755250930786,
-0.06896005570888519,
-0.014912458136677742,
0.047170668840408325,
-0.033234212547540665,
-0.006792176980525255,
-0.025113990530371666,
-0.010330410674214363,
-0.010350807569921017,
0.00766789773479104,
0.03235587850213051,
-0.05232540890574455,
0.09223734587430954,
-0.016396554186940193,
-0.02996980771422386,
0.00336210778914392,
0.03183334693312645,
0.01771998032927513,
-0.010326351970434189,
0.025009535253047943,
0.015361746773123741,
0.04032336175441742,
-0.04471499100327492,
0.014250516891479492,
0.010487331077456474,
-0.019461024552583694,
0.017809094861149788,
-0.34566229581832886,
-0.0010523187229409814,
0.07394495606422424,
0.0330486074090004,
0.019928060472011566,
-0.018975786864757538,
0.019664432853460312,
-0.016225045546889305,
-0.03305274620652199,
0.0742647647857666,
0.0032206957694143057,
-0.01270264945924282,
-0.015030118636786938,
0.038253918290138245,
0.029376180842518806,
0.017339719459414482,
-0.002440027892589569,
-0.04775693640112877,
0.012268517166376114,
-0.024457644671201706,
-0.014901877380907536,
-0.034139540046453476,
0.0030393311753869057,
-0.06686636805534363,
0.04764072224497795,
0.02109762839972973,
0.0909653753042221,
-0.005939982831478119,
-0.026736117899417877,
0.06988561153411865,
0.028846316039562225,
0.04295225813984871,
0.04205695539712906,
-0.1262013018131256,
0.04518134891986847,
0.0062294513918459415,
-0.04291875660419464,
0.0025974861346185207,
-0.024355903267860413,
-0.03914680331945419,
-0.05759407579898834,
0.004788020625710487,
-0.055677589029073715,
-0.023855693638324738,
-0.06702131032943726,
-0.007345371879637241,
0.03613002225756645,
0.023685775697231293,
-0.037521012127399445,
-0.03608335927128792,
0.03405104577541351,
0.018622523173689842,
-0.031249139457941055,
-0.01094511803239584,
-0.0018758588703349233,
-0.00958090741187334,
-0.08016302436590195,
0.05570799484848976,
-0.011780298314988613,
-0.05036560818552971,
-0.029104631394147873,
-0.011015704832971096,
0.047913238406181335,
-0.0822567492723465,
0.002656854223459959,
-0.02184567227959633,
0.026231572031974792,
-0.006229456979781389,
-0.00771080469712615,
0.04622612148523331,
-0.0267044585198164,
0.06471064686775208,
-0.030936293303966522,
-0.03646516799926758,
0.014870318584144115,
0.025518789887428284,
-0.048539645969867706,
-0.02081354334950447,
-0.09328629821538925,
0.001186106470413506,
-0.020298965275287628,
-0.046564459800720215,
0.027172626927495003,
0.03675774857401848,
0.023927997797727585,
-0.014703715220093727,
-0.022491147741675377,
-0.03069417178630829,
0.01965435966849327,
0.028179163113236427,
0.005070572718977928,
0.013617522083222866,
0.01926194317638874,
0.030568955466151237,
0.03707178682088852,
0.032931141555309296,
-0.23657755553722382,
-0.05086570978164673,
-0.004557126201689243,
0.07215352356433868,
-0.03627347946166992,
0.09727945923805237,
-0.026145879179239273,
0.052512336522340775,
0.0339818075299263,
-0.032199978828430176,
0.015219185501337051,
-0.03516840934753418,
0.05224469676613808,
0.02352811023592949,
-0.023352626711130142,
0.02439219318330288,
0.02372543327510357,
-0.05147809907793999,
-0.0030183775816112757,
0.027544626966118813,
0.06885460764169693,
-0.0042079440318048,
0.10417808592319489,
-0.03265167772769928,
0.0001571304746903479,
-0.02165760099887848,
-0.031069742515683174,
-0.10689553618431091,
0.019986139610409737,
-0.019830437377095222,
0.04106457903981209,
-0.00883906427770853,
0.053016625344753265,
0.08016037940979004,
0.05730737745761871,
0.00002947996836155653,
-0.04784691333770752,
-0.017872564494609833,
-0.033841319382190704,
0.02023627795279026,
-0.05496932566165924,
0.06352203339338303,
0.020750727504491806,
-0.04885480925440788,
0.06766828894615173,
-0.023100875318050385,
0.018627898767590523,
-0.0668676570057869,
-0.006793764419853687,
-0.024356285110116005,
0.012271351180970669,
-0.0019707120954990387,
0.04016820713877678,
0.04881720617413521,
0.003209851449355483,
0.025769062340259552,
-0.08831660449504852,
-0.04699238762259483,
0.035281796008348465,
-0.014168153516948223,
-0.004615006037056446,
0.039941683411598206,
0.019667765125632286,
0.05854492634534836,
0.07430082559585571
] |
PMID:25027 | Neuroendocrine carcinomas of the colon. Ultrastructural and biochemical evidence of their secretory function. | Four cases of malignant colonic tumors diagnosed by light microscopy as "small cell undifferentiated carcinomas" were shown by electron microscopy to have neurosecretory-type granules. Biochemical analysis of tumor tissue extracts disclosed the presence of considerable levels of VMA and catecholamines in all tumors; 5-HIAA was present in one tumor. Clinically, there had been no signs or symptoms attributable to those or related substances. Similar observations have been reported in a variety of neuroendocrine neoplasms; for example, the demonstration of neurosecretory-type granules and determination of amine or peptide materials in tumor tissue or body fluids may not be necessarily reflected in clinical hormonal syndromes or obvious metabolic abnormalities. Our structural and biochemical observations indicate that, regardless of clinically evident hormonal activity or lack thereof, some small cell "undifferentiated" colonic cancers derive from APUD elements, and therefore they should be classified within the group of neuroendocrine carcinomas. The evident secretory capabilities of these carcinomas suggest obvious diagnostic possibilities and could conceivably lead to a reappraisal of current therapy. | Neuroendocrine carcinomas of the colon. Ultrastructural and biochemical evidence of their secretory function. Four cases of malignant colonic tumors diagnosed by light microscopy as "small cell undifferentiated carcinomas" were shown by electron microscopy to have neurosecretory-type granules. Biochemical analysis of tumor tissue extracts disclosed the presence of considerable levels of VMA and catecholamines in all tumors; 5-HIAA was present in one tumor. Clinically, there had been no signs or symptoms attributable to those or related substances. Similar observations have been reported in a variety of neuroendocrine neoplasms; for example, the demonstration of neurosecretory-type granules and determination of amine or peptide materials in tumor tissue or body fluids may not be necessarily reflected in clinical hormonal syndromes or obvious metabolic abnormalities. Our structural and biochemical observations indicate that, regardless of clinically evident hormonal activity or lack thereof, some small cell "undifferentiated" colonic cancers derive from APUD elements, and therefore they should be classified within the group of neuroendocrine carcinomas. The evident secretory capabilities of these carcinomas suggest obvious diagnostic possibilities and could conceivably lead to a reappraisal of current therapy. | [
-0.02167992666363716,
-0.02438780479133129,
0.03769419342279434,
0.018780168145895004,
0.049365364015102386,
0.014488360844552517,
0.08720133453607559,
0.02218778058886528,
-0.01584545522928238,
-0.02738264948129654,
0.03655647113919258,
0.0010219813557341695,
-0.022100355476140976,
0.03555377200245857,
-0.03300746530294418,
-0.024514848366379738,
0.029820740222930908,
-0.03734111413359642,
-0.007632435765117407,
-0.004324769601225853,
0.02673381380736828,
-0.015352821908891201,
0.014638262800872326,
0.0010064328089356422,
0.03895009309053421,
-0.008324501104652882,
0.022038647904992104,
0.007415114436298609,
-0.09847106784582138,
-0.1555543839931488,
0.0114879896864295,
-0.00811123475432396,
0.051031697541475296,
-0.047321733087301254,
0.032842252403497696,
-0.02108185924589634,
-0.014174624346196651,
0.0025432121474295855,
-0.03478742018342018,
0.014036339707672596,
0.06785188615322113,
-0.02489425055682659,
-0.004006579518318176,
0.014936220832169056,
0.03667200356721878,
0.0015038716373965144,
-0.032165560871362686,
-0.049389783293008804,
0.05618816241621971,
0.04339727386832237,
0.05767614394426346,
-0.034663427621126175,
-0.02785417065024376,
0.07825138419866562,
0.03676031902432442,
-0.017096728086471558,
0.028399022296071053,
-0.008820943534374237,
-0.03339065611362457,
0.03413498029112816,
0.0037275964859873056,
0.14089995622634888,
-0.1189127117395401,
0.012786133214831352,
0.08194245398044586,
-0.0466386117041111,
-0.08104472607374191,
-0.04071621969342232,
0.08454769849777222,
-0.010901796631515026,
0.025895599275827408,
0.030735008418560028,
0.021618375554680824,
0.05151534825563431,
-0.023349501192569733,
-0.06023483723402023,
0.028129274025559425,
-0.023333627730607986,
-0.03620290383696556,
0.051363930106163025,
0.011685008183121681,
0.054904915392398834,
0.02967597357928753,
-0.043313924223184586,
-0.01802811026573181,
-0.04958314448595047,
-0.08175290375947952,
-0.03930265083909035,
-0.016417641192674637,
-0.013149638660252094,
-0.033959560096263885,
-0.0712277814745903,
0.004070911556482315,
-0.008233713917434216,
-0.05824289098381996,
-0.0643569678068161,
-0.026103084906935692,
-0.026265336200594902,
0.016750900074839592,
0.3789358139038086,
-0.060343362390995026,
0.009806697256863117,
0.02110927738249302,
-0.013166696764528751,
-0.025582442060112953,
0.039911601692438126,
-0.05640875920653343,
0.009293495677411556,
0.007697781082242727,
0.0841449722647667,
0.03206682205200195,
0.01730984076857567,
0.007129390258342028,
-0.07907768338918686,
-0.016498081386089325,
-0.03321133181452751,
0.040851663798093796,
-0.0037703842390328646,
-0.05449346825480461,
0.029565291479229927,
-0.013618533499538898,
-0.05507988855242729,
0.02784743346273899,
-0.004139831755310297,
0.04265769571065903,
0.0003238527860958129,
-0.040045179426670074,
0.029341528192162514,
0.045362889766693115,
0.005189018789678812,
-0.001432117074728012,
0.050694894045591354,
-0.019185258075594902,
-0.014907482080161572,
0.01065772119909525,
-0.011533304117619991,
0.0752386525273323,
-0.016500959172844887,
-0.013314316980540752,
-0.027869289740920067,
-0.022637415677309036,
-0.07020632922649384,
-0.0009407607140019536,
-0.05132113769650459,
-0.04558858647942543,
0.08656623959541321,
0.015571816824376583,
-0.0021647175308316946,
-0.002846021670848131,
0.044319622218608856,
0.00201645796187222,
0.10932473838329315,
-0.02758241817355156,
0.04923264682292938,
-0.026288704946637154,
0.008710408583283424,
-0.011670855805277824,
-0.031220724806189537,
-0.050488971173763275,
-0.006097679026424885,
0.007446685340255499,
-0.00027652649441733956,
-0.005708106327801943,
0.070500947535038,
0.0612674318253994,
-0.009991605766117573,
-0.058986764401197433,
-0.048703692853450775,
0.010824964381754398,
0.0021491344086825848,
0.02241058088839054,
0.06695996224880219,
-0.0421178825199604,
-0.027081305161118507,
-0.05346962437033653,
0.00678597716614604,
-0.0018163464264944196,
0.011260125786066055,
0.02267131209373474,
0.03610174357891083,
-0.01802196353673935,
-0.0132838049903512,
-0.004037634469568729,
0.04527018591761589,
0.01689452864229679,
-0.007066657301038504,
-0.016814852133393288,
-0.010482404381036758,
0.038100387901067734,
0.04161401465535164,
-0.04380132630467415,
-0.015871424227952957,
-0.0991055890917778,
0.0002475967921782285,
-0.07407457381486893,
-0.0463259182870388,
-0.0017408068524673581,
-0.02923230454325676,
-0.036621030420064926,
-0.004859088454395533,
0.05285894125699997,
0.02452033758163452,
0.03710095211863518,
0.09117620438337326,
0.03753410279750824,
0.033734679222106934,
0.0014137080870568752,
0.040393535047769547,
0.022362928837537766,
-0.02558886632323265,
-0.016964413225650787,
0.007394010666757822,
-0.034473709762096405,
0.012532918713986874,
-0.042476292699575424,
0.013691439293324947,
-0.017858626320958138,
0.017782414332032204,
0.05194507911801338,
-0.06163790822029114,
0.02832951210439205,
-0.023518458008766174,
0.01607225090265274,
-0.30354785919189453,
-0.07693671435117722,
-0.006949517410248518,
-0.09291843324899673,
0.023495446890592575,
-0.01967712491750717,
0.0037275529466569424,
-0.0042270477861166,
0.005394999403506517,
-0.05356549844145775,
0.01655036397278309,
0.07129097729921341,
-0.019054044038057327,
-0.016591109335422516,
-0.024977829307317734,
0.05491716042160988,
0.04842904955148697,
-0.057237572968006134,
0.017421839758753777,
0.038296040147542953,
0.0206194706261158,
0.020029667764902115,
0.014269469305872917,
0.020082460716366768,
0.02400808595120907,
-0.003711165627464652,
0.12918797135353088,
0.05581754073500633,
0.0032151213381439447,
0.015427726320922375,
0.006636008620262146,
0.06089310348033905,
-0.03869181498885155,
-0.0354955829679966,
0.0286709051579237,
-0.13767226040363312,
-0.07221468538045883,
0.0006852103979326785,
-0.0710698738694191,
-0.010440843179821968,
-0.024467526003718376,
-0.03196263313293457,
-0.00927369948476553,
-0.08629651367664337,
-0.013629825785756111,
-0.035232722759246826,
-0.007559424266219139,
0.03363566845655441,
-0.015508058480918407,
-0.00773650174960494,
0.10178990662097931,
-0.014387078583240509,
0.018060030415654182,
-0.0021014090161770582,
0.023014897480607033,
-0.021072126924991608,
-0.03971964865922928,
-0.009485646151006222,
-0.0813872367143631,
0.04404392093420029,
0.03928438574075699,
0.02934916689991951,
-0.015743911266326904,
0.0034080008044838905,
0.0060880146920681,
0.013586053624749184,
0.00661148177459836,
-0.016947366297245026,
0.07314805686473846,
-0.007052197586745024,
0.026143185794353485,
0.13893020153045654,
0.0038000191561877728,
0.013005537912249565,
0.048785701394081116,
-0.009089051745831966,
-0.007201381027698517,
-0.027790118008852005,
-0.02541862614452839,
0.03656909242272377,
-0.004008166026324034,
-0.02095533162355423,
0.08803824335336685,
0.008783848024904728,
-0.039555393159389496,
0.04294656589627266,
0.05061298981308937,
-0.029923532158136368,
0.006643336266279221,
-0.006053428631275892,
0.014694142155349255,
0.06256716698408127,
-0.015289860777556896,
-0.04664558917284012,
0.017009250819683075,
-0.010129515081644058,
-0.28476688265800476,
0.028329258784651756,
0.01796090602874756,
0.024162394925951958,
-0.048066262155771255,
-0.0007343554752878845,
-0.04549860581755638,
0.0032131837215274572,
-0.01676921732723713,
0.050166863948106766,
0.043124932795763016,
-0.020263882353901863,
0.0825723335146904,
-0.034287769347429276,
-0.030623463913798332,
-0.0010784565238282084,
0.025360632687807083,
-0.010782422497868538,
0.05452999100089073,
0.003800265723839402,
0.05120910704135895,
-0.019978266209363937,
0.1467946469783783,
-0.024866996333003044,
-0.06699681282043457,
-0.00798873696476221,
-0.003724155016243458,
0.03089958243072033,
0.006342281587421894,
0.02539367973804474,
0.07080480456352234,
0.04630172625184059,
0.0054258788004517555,
0.018893852829933167,
0.0020654890686273575,
0.011000528000295162,
0.024997010827064514,
0.026564043015241623,
-0.03246152028441429,
0.03985334187746048,
-0.009620649740099907,
0.017916183918714523,
-0.12684227526187897,
-0.03883631154894829,
0.018797628581523895,
-0.08494216203689575,
0.00015558733139187098,
-0.06637711822986603,
0.0060237315483391285,
-0.04460631310939789,
-0.005692026577889919,
-0.012072740122675896,
0.039203956723213196,
0.023426083847880363,
0.024463312700390816,
-0.0023775859735906124,
0.013639991171658039,
-0.019791020080447197,
0.04533282667398453,
-0.018117299303412437,
0.0014599483693018556,
-0.0019392051035538316,
-0.04866611212491989,
0.09534566849470139,
-0.0022049578838050365
] |
PMID:25029 | Biochemical tests for the detection of alcoholism: comparison of plasma alpha-amino-n-butyric acid with other available tests. | The measurement of AANB relative to leucine was found to be a useful biochemical test for the detection of chronic alcohol consumption. This test, in combination with GGTP determinations, provided a sensitive means of detection (81% positive) for chronic alcohol consumption with a minimum of false positive determinations (2%). | Biochemical tests for the detection of alcoholism: comparison of plasma alpha-amino-n-butyric acid with other available tests. The measurement of AANB relative to leucine was found to be a useful biochemical test for the detection of chronic alcohol consumption. This test, in combination with GGTP determinations, provided a sensitive means of detection (81% positive) for chronic alcohol consumption with a minimum of false positive determinations (2%). | [
0.00016622843395452946,
0.04813612252473831,
0.003297650022432208,
-0.040996003895998,
0.06314706057310104,
-0.023609157651662827,
0.10466473549604416,
0.027450604364275932,
-0.02400512807071209,
-0.04153456538915634,
0.03068341687321663,
-0.06596893072128296,
-0.02732243575155735,
0.06279554218053818,
-0.05677393823862076,
0.020134883001446724,
0.0378895029425621,
0.033029280602931976,
0.036207497119903564,
0.0001881415373645723,
-0.013342777267098427,
0.03788251429796219,
0.03906131535768509,
-0.036241017282009125,
0.04419633001089096,
0.07333188503980637,
0.02023547887802124,
-0.021462561562657356,
-0.0829877108335495,
-0.12953650951385498,
-0.02036503329873085,
0.000733646156731993,
0.012485800310969353,
-0.0666099563241005,
-0.038742873817682266,
-0.0033601203467696905,
-0.04635502025485039,
0.014974900521337986,
-0.03167688474059105,
0.0006746795843355358,
0.046049222350120544,
-0.019260963425040245,
-0.0352335125207901,
-0.006058454047888517,
0.031108738854527473,
0.024606147781014442,
-0.004702522419393063,
0.04353012144565582,
-0.007008194923400879,
0.03916412219405174,
0.012480233795940876,
0.019449947401881218,
0.08369547128677368,
0.036288876086473465,
0.00869759637862444,
-0.023550305515527725,
-0.03858370706439018,
-0.002511277562007308,
-0.06282056123018265,
0.02976996824145317,
-0.013253997080028057,
-0.010976096615195274,
-0.1426180750131607,
0.02913234941661358,
0.03799844905734062,
0.02541397325694561,
-0.08524556457996368,
0.008656344376504421,
0.024668097496032715,
0.025495953857898712,
-0.09403505176305771,
0.005835302639752626,
0.022437218576669693,
0.024978268891572952,
-0.01873791590332985,
-0.03149757161736488,
-0.0003190806892234832,
-0.028706056997179985,
0.03781527653336525,
-0.009103014133870602,
0.015734385699033737,
0.030318882316350937,
0.046391651034355164,
-0.05946354940533638,
0.043227165937423706,
-0.04839963838458061,
0.011091884225606918,
0.023430325090885162,
0.05677347630262375,
0.04300539195537567,
-0.01836756058037281,
-0.03469420596957207,
-0.017443913966417313,
0.008681497536599636,
-0.043453801423311234,
-0.05226502940058708,
0.03454018756747246,
-0.004084159154444933,
-0.015521146357059479,
0.36413130164146423,
0.008478628471493721,
0.01596354879438877,
-0.05087602511048317,
-0.05108359083533287,
-0.0115647092461586,
-0.024898778647184372,
-0.039353616535663605,
0.04780643805861473,
-0.034922562539577484,
0.07199546694755554,
0.05030925199389458,
0.020297067239880562,
0.04591726139187813,
-0.04182274267077446,
-0.0508660189807415,
0.040247123688459396,
0.057186730206012726,
-0.009498294442892075,
-0.00928749144077301,
0.05327916145324707,
-0.034079812467098236,
-0.01854698359966278,
0.019609767943620682,
-0.08063699305057526,
0.0013380845775827765,
-0.02122911810874939,
0.005565621890127659,
0.06545237451791763,
0.034425802528858185,
0.04454478621482849,
0.036329351365566254,
-0.04286228492856026,
-0.00331355188973248,
-0.022730452939867973,
-0.028923237696290016,
-0.014924751594662666,
0.04194345325231552,
0.0156526081264019,
0.033581577241420746,
0.0766262337565422,
-0.040312398225069046,
-0.005042708478868008,
0.012009900994598866,
-0.043490294367074966,
-0.04130617156624794,
0.01147277932614088,
0.015615926124155521,
0.021145641803741455,
0.0005804542452096939,
-0.03057183511555195,
0.04396268352866173,
0.04238790646195412,
-0.0480639785528183,
-0.022727111354470253,
0.01215435191988945,
0.024226516485214233,
-0.002712163608521223,
-0.011177582666277885,
-0.023780468851327896,
-0.013701951131224632,
-0.06778080016374588,
-0.029959527775645256,
-0.011705624870955944,
0.024213159456849098,
0.04389151185750961,
-0.04754370450973511,
-0.0134245241060853,
-0.01951724849641323,
-0.016001345589756966,
-0.013363071717321873,
0.03837273269891739,
0.01827237568795681,
-0.00586347421631217,
0.019570499658584595,
-0.02756497822701931,
-0.041822321712970734,
0.020669931545853615,
0.08999769389629364,
-0.0028923754580318928,
-0.028213700279593468,
0.07386079430580139,
-0.014883744530379772,
-0.07374316453933716,
0.07686269283294678,
0.05539045110344887,
0.002394132548943162,
-0.03466224670410156,
-0.03339402377605438,
0.05535849928855896,
0.017079411074519157,
-0.05456486716866493,
0.03263895958662033,
-0.09915492683649063,
-0.004187015350908041,
-0.014189069159328938,
-0.0140905836597085,
-0.018103674054145813,
-0.03499554842710495,
-0.00889749638736248,
0.004915743134915829,
0.06595264375209808,
0.017943715676665306,
0.019383171573281288,
0.024216894060373306,
0.07368884235620499,
0.034278400242328644,
-0.015323401428759098,
-0.02547919563949108,
0.03531754016876221,
0.018915941938757896,
-0.0236765518784523,
-0.00497351074591279,
-0.03740118816494942,
-0.019884368404746056,
-0.0728742703795433,
-0.006292780861258507,
0.014380719512701035,
-0.02728958986699581,
-0.0012001378927379847,
0.007056545931845903,
0.04269644618034363,
-0.07296225428581238,
0.026265747845172882,
-0.3076618015766144,
-0.014640492387115955,
0.05414559319615364,
0.04249123856425285,
0.047405682504177094,
-0.062054071575403214,
-0.041070956736803055,
-0.01689831353724003,
-0.023591570556163788,
0.002235477091744542,
0.030704056844115257,
0.09278522431850433,
-0.003986406605690718,
-0.024420663714408875,
-0.00498163141310215,
0.06527413427829742,
0.0057646045461297035,
-0.06162906065583229,
0.011667211540043354,
0.00905628316104412,
-0.009422504343092442,
-0.021143468096852303,
0.0224223081022501,
0.024714356288313866,
0.07224731147289276,
-0.08256743848323822,
0.1405775547027588,
-0.013525950722396374,
0.009252991527318954,
0.007075710222125053,
-0.04328038915991783,
0.03824441134929657,
-0.007889085449278355,
-0.02913850173354149,
-0.004732197616249323,
-0.009158872067928314,
-0.06824610382318497,
0.008140035904943943,
-0.024073153734207153,
-0.024359486997127533,
0.018879858776926994,
0.02659784071147442,
-0.017839718610048294,
-0.10133586823940277,
-0.05700371414422989,
-0.017673857510089874,
0.04997299239039421,
-0.0008995865355245769,
-0.020525116473436356,
-0.037417858839035034,
0.06638460606336594,
-0.014365906827151775,
0.0317351371049881,
-0.03321857377886772,
0.023409586399793625,
-0.009323333390057087,
-0.06424151360988617,
-0.06297831237316132,
-0.074678435921669,
0.0023941562976688147,
0.06473912298679352,
0.0717683956027031,
-0.0305156372487545,
0.005568967666476965,
0.04217716306447983,
0.02738274447619915,
-0.053679317235946655,
-0.013764134608209133,
0.09606890380382538,
0.04049212485551834,
0.08481931686401367,
0.08052991330623627,
-0.043634697794914246,
-0.021023599430918694,
0.04099360480904579,
0.0016441617626696825,
0.015340245328843594,
-0.08849646151065826,
-0.04096239432692528,
0.013750585727393627,
-0.03519873693585396,
-0.054050128906965256,
0.06743678450584412,
-0.038950856775045395,
0.0070894150994718075,
0.058253202587366104,
0.03487658128142357,
0.006165745202451944,
0.0538165420293808,
0.005644642747938633,
0.044583674520254135,
-0.002989468164741993,
-0.0035857073962688446,
-0.09457507729530334,
-0.01433203648775816,
0.03942146897315979,
-0.2807683050632477,
0.0024086046032607555,
-0.034956905990839005,
0.0030117223504930735,
-0.06843001395463943,
0.002609795657917857,
-0.017546942457556725,
0.03282669186592102,
-0.050843335688114166,
0.011430252343416214,
-0.00838937982916832,
-0.004458054434508085,
0.0663476288318634,
-0.03396274149417877,
0.00469284038990736,
0.005774701479822397,
0.03217247501015663,
-0.03228522837162018,
-0.0364285483956337,
-0.007841397076845169,
0.014905329793691635,
-0.01020788587629795,
0.1449887603521347,
-0.030678028240799904,
-0.03721027448773384,
0.060215532779693604,
0.03690328076481819,
-0.003065644297748804,
-0.02391810528934002,
-0.016795646399259567,
0.012369108386337757,
0.041649434715509415,
0.10965969413518906,
-0.04619596153497696,
0.056232843548059464,
0.11185058951377869,
-0.03001052513718605,
0.006764349527657032,
-0.007515173405408859,
-0.01675242744386196,
0.04592364281415939,
-0.024393025785684586,
-0.08124447613954544,
-0.05635715648531914,
0.0449514277279377,
-0.037022415548563004,
-0.06935001164674759,
-0.0001537607458885759,
-0.011837892234325409,
0.015517660416662693,
-0.01909569650888443,
0.01737557351589203,
0.03313580900430679,
0.005966958124190569,
0.017528889700770378,
-0.028267590329051018,
-0.08945979177951813,
0.00710057420656085,
0.01898723468184471,
-0.03037741407752037,
-0.00040004434413276613,
0.06810329854488373,
0.018221737816929817,
0.11262901872396469,
0.018873829394578934
] |
PMID:25030 | Pain and clinical thrombophlebitis following intravenous diazepam and lorazepam. | Eighty-seven per cent of surgical patients receiving undiluted diazepam experienced pain on injection while 6-16%, depending on the dose, manifested evidence of clinical thrombophlebitis. This was improved when diazepam, 10 mg, was diluted to 20-40 ml with intravenous solution. In contrast, lorazepam appeared to have minimal irritative or injurious effects on veins whether undiluted or diluted. In view of these results and clinical studies reporting a higher patient acceptance of lorazepam than diazepam, lorazepam may be a superior drug for use in anaesthesia. | Pain and clinical thrombophlebitis following intravenous diazepam and lorazepam. Eighty-seven per cent of surgical patients receiving undiluted diazepam experienced pain on injection while 6-16%, depending on the dose, manifested evidence of clinical thrombophlebitis. This was improved when diazepam, 10 mg, was diluted to 20-40 ml with intravenous solution. In contrast, lorazepam appeared to have minimal irritative or injurious effects on veins whether undiluted or diluted. In view of these results and clinical studies reporting a higher patient acceptance of lorazepam than diazepam, lorazepam may be a superior drug for use in anaesthesia. | [
-0.02991524338722229,
-0.005449206568300724,
0.03730897605419159,
0.041567426174879074,
0.02066233567893505,
-0.02430449053645134,
0.06923992186784744,
0.08084522932767868,
0.002767552388831973,
-0.023178046569228172,
-0.03240460529923439,
0.03359418362379074,
-0.030875157564878464,
0.02046959474682808,
-0.013727553188800812,
-0.01673314720392227,
0.04845099896192551,
-0.058662671595811844,
-0.06280776858329773,
0.04131066054105759,
-0.002541558351367712,
-0.008321074768900871,
0.025898326188325882,
0.02987079694867134,
-0.03925847262144089,
0.002764487871900201,
0.0434744618833065,
0.015963329002261162,
0.019600393250584602,
-0.18025967478752136,
0.046744950115680695,
0.0029140205588191748,
-0.0013336887350305915,
-0.050590820610523224,
-0.020652243867516518,
0.021903101354837418,
-0.039419177919626236,
0.03800276666879654,
0.01227243710309267,
0.008984042331576347,
0.11541613191366196,
0.03121117316186428,
-0.029280629009008408,
0.007594494614750147,
0.01359385997056961,
-0.04630506411194801,
-0.03080672025680542,
0.019153742119669914,
0.1392972320318222,
0.009778302162885666,
0.055074743926525116,
-0.033274609595537186,
0.016000356525182724,
0.09517164528369904,
-0.034488845616579056,
-0.07509130239486694,
-0.029809873551130295,
-0.04521091654896736,
-0.019199959933757782,
0.034932252019643784,
-0.009994893334805965,
0.01875375211238861,
-0.1196824163198471,
0.026360822841525078,
-0.005396869033575058,
-0.00007912801811471581,
0.012868617661297321,
-0.01860054023563862,
-0.032207638025283813,
0.09920936822891235,
0.00015446571342181414,
0.03956162557005882,
0.03272233530879021,
0.052838362753391266,
0.027619298547506332,
-0.02690667100250721,
0.09388229250907898,
0.028330104425549507,
-0.044838324189186096,
-0.015612954273819923,
-0.004411649890244007,
-0.022358672693371773,
-0.003479111474007368,
-0.083249531686306,
-0.047245923429727554,
-0.08247652649879456,
0.04111639782786369,
-0.021467169746756554,
0.013667372055351734,
0.05116468667984009,
0.056960877031087875,
-0.010101386345922947,
-0.03637504205107689,
-0.07422811537981033,
-0.00015392446948681027,
0.0021271102596074343,
-0.01739303208887577,
-0.025893373414874077,
-0.002800887217745185,
0.3477360010147095,
-0.022094273939728737,
0.008988668210804462,
-0.03878779709339142,
-0.027774538844823837,
0.0030681814532727003,
-0.0035956287756562233,
0.01828446239233017,
0.004851477220654488,
-0.02387724071741104,
0.01922145113348961,
0.01908571831882,
0.006520651280879974,
0.11822669953107834,
0.0015032782685011625,
-0.06226757913827896,
0.035361725836992264,
0.05623168870806694,
-0.025021398440003395,
0.006387136876583099,
0.03776491433382034,
-0.011681105010211468,
-0.004358194302767515,
-0.0235143955796957,
-0.056140027940273285,
0.05454263091087341,
-0.0021622981876134872,
-0.019256262108683586,
0.0820467621088028,
0.013012968935072422,
0.04384041205048561,
0.023661138489842415,
-0.09443189203739166,
0.02519902028143406,
0.07095730304718018,
0.019653381779789925,
-0.023243127390742302,
0.03126051649451256,
0.0011460705427452922,
0.04748446121811867,
-0.051478348672389984,
0.029483916237950325,
-0.048846740275621414,
0.000493394851218909,
-0.07031770050525665,
-0.08059268444776535,
0.08058144152164459,
-0.02806074544787407,
0.014952842146158218,
-0.03452198952436447,
-0.07206995040178299,
-0.03721357882022858,
0.033750008791685104,
-0.05812845751643181,
0.07271809130907059,
-0.04156394675374031,
0.04749275743961334,
-0.02225898765027523,
0.046241648495197296,
-0.04686510190367699,
0.04480923339724541,
-0.016064124181866646,
-0.054275915026664734,
-0.006322356406599283,
0.03012593276798725,
-0.027063654735684395,
0.044194284826517105,
-0.033038243651390076,
-0.003159307176247239,
-0.014255451038479805,
0.06346326321363449,
-0.00910175684839487,
0.01908697932958603,
-0.028916776180267334,
0.0005889895837754011,
0.01553780771791935,
0.023670444265007973,
0.05233876034617424,
-0.033650755882263184,
0.004278722684830427,
-0.005019907373934984,
0.022116409614682198,
0.0838252604007721,
-0.013319241814315319,
0.020627839490771294,
0.027838408946990967,
0.015897471457719803,
-0.008482695557177067,
0.028126323595643044,
0.0023577329702675343,
0.01865612342953682,
-0.047451797872781754,
-0.02950458414852619,
-0.0456593818962574,
0.028117170557379723,
-0.009572645649313927,
-0.012709511443972588,
-0.0835583284497261,
-0.027607234194874763,
-0.04544045776128769,
-0.03962438553571701,
0.10335129499435425,
0.05890278518199921,
-0.009449725970625877,
0.07504140585660934,
0.02503250166773796,
0.06273098289966583,
0.004691835958510637,
-0.04352647066116333,
0.030904045328497887,
0.008665877394378185,
0.04650659114122391,
0.015299239195883274,
0.018252180889248848,
-0.010927574709057808,
-0.04225921630859375,
0.012978917919099331,
0.013433803804218769,
0.015990128740668297,
-0.010928389616310596,
0.027640588581562042,
-0.026063956320285797,
-0.04892712086439133,
0.011303805746138096,
-0.3165975511074066,
-0.03171433135867119,
0.05763348564505577,
0.03741374611854553,
0.07167422771453857,
-0.026781421154737473,
0.022712644189596176,
-0.03897707909345627,
-0.06212575361132622,
-0.006667917128652334,
-0.03281545639038086,
0.04425248131155968,
-0.09074170887470245,
-0.05494244396686554,
0.008415701799094677,
-0.006498986389487982,
0.06713756173849106,
-0.010828287340700626,
0.029535412788391113,
-0.007348916959017515,
-0.0018504941835999489,
-0.023243524134159088,
-0.03767582029104233,
0.035960935056209564,
0.0386524461209774,
0.028339732438325882,
0.10273657739162445,
-0.004633652046322823,
-0.0753759890794754,
-0.06836871802806854,
0.015357356518507004,
0.07524358481168747,
-0.02183556742966175,
-0.11458627134561539,
-0.0037083118222653866,
-0.0024584983475506306,
0.03737378865480423,
0.015229768119752407,
0.015273046679794788,
-0.0061297048814594746,
-0.10965228825807571,
-0.030698472633957863,
-0.01325430627912283,
-0.048495519906282425,
-0.025967897847294807,
-0.03212716430425644,
-0.007079901173710823,
-0.005595726426690817,
-0.03504953160881996,
-0.03024831786751747,
0.010496825911104679,
-0.006701950915157795,
0.004097026772797108,
-0.020951975136995316,
0.041106969118118286,
-0.037151701748371124,
-0.07754696905612946,
0.02476748451590538,
0.032364651560783386,
-0.026410946622490883,
0.0036869547329843044,
0.019098438322544098,
0.0568576455116272,
-0.06608058512210846,
-0.024597221985459328,
0.017702028155326843,
0.05264335125684738,
0.015666063874959946,
0.09072000533342361,
0.016126230359077454,
-0.023357274010777473,
0.12181999534368515,
-0.047148868441581726,
-0.0725724846124649,
0.06912815570831299,
0.008920343592762947,
-0.038611724972724915,
0.04303916543722153,
-0.08343245834112167,
0.017061615362763405,
0.02602470852434635,
0.03851746395230293,
0.04802095144987106,
0.0349302664399147,
-0.03267769142985344,
0.029875515028834343,
0.0010741162113845348,
-0.0228698942810297,
-0.021687563508749008,
-0.05478055402636528,
0.006011536344885826,
-0.030793270096182823,
-0.01874370127916336,
-0.014492010697722435,
-0.008497226983308792,
-0.0104491813108325,
-0.24644775688648224,
-0.03594827279448509,
0.03616335242986679,
0.012254022993147373,
-0.012330049648880959,
0.013322648592293262,
0.08877457678318024,
-0.03196209296584129,
-0.00936854723840952,
-0.023850759491324425,
-0.001974973361939192,
-0.028304066509008408,
-0.0011636941926553845,
0.01978422701358795,
-0.05532235652208328,
0.035190362483263016,
0.07438862323760986,
-0.01727203279733658,
0.0020104246214032173,
-0.01646091602742672,
0.021481331437826157,
0.019669203087687492,
0.06890638917684555,
0.043512243777513504,
0.015617724508047104,
0.0690561830997467,
0.03080187737941742,
0.058252494782209396,
-0.056798212230205536,
0.03471394255757332,
-0.027103690430521965,
0.006842115428298712,
0.08846317231655121,
-0.021145572885870934,
0.029389312490820885,
0.0186579842120409,
-0.11455155164003372,
-0.0012691962765529752,
0.04925061762332916,
0.006439263001084328,
0.013948500156402588,
0.04302971437573433,
-0.001254844362847507,
0.017464376986026764,
0.013121221214532852,
-0.06740143895149231,
-0.020522743463516235,
-0.07154592126607895,
0.007184983696788549,
-0.036635205149650574,
-0.02861645258963108,
-0.0103838499635458,
0.03253183513879776,
0.04809509962797165,
0.05659234896302223,
-0.0233086496591568,
-0.014979870989918709,
-0.021516790613532066,
0.007599830627441406,
0.002589958719909191,
-0.07978784292936325,
-0.04863752797245979,
-0.013337517157196999,
0.052713505923748016,
-0.044139567762613297
] |
PMID:25031 | Arterialized capillary blood used to determine the acid-base and blood gas status of dogs. | Blood samples were collected by microhematocrit tube from posterior medial margin of the shaved, but otherwise untreated, canine ear. Acid-base and blood gas values of these samples were compared with the values of samples obtained simultaneously from the carotid artery. The arterialized nature of capillary blood from the canine ear was demonstrated under various degrees of chemical restraint and during conditions of extreme hypoxic acidosis to hyperventilatory alkalosis. Once a week determinations of acid-base and blood gas status with such arterialized capillary blood from a group of awake dogs showed within-subject variance to be significantly less (P less than 0.05) than between-subject variance; thus, uniqueness of individual dogs was reliably revealed. This technique also was used to demonstrated breed differences for acid-base and blood gas characteristics. | Arterialized capillary blood used to determine the acid-base and blood gas status of dogs. Blood samples were collected by microhematocrit tube from posterior medial margin of the shaved, but otherwise untreated, canine ear. Acid-base and blood gas values of these samples were compared with the values of samples obtained simultaneously from the carotid artery. The arterialized nature of capillary blood from the canine ear was demonstrated under various degrees of chemical restraint and during conditions of extreme hypoxic acidosis to hyperventilatory alkalosis. Once a week determinations of acid-base and blood gas status with such arterialized capillary blood from a group of awake dogs showed within-subject variance to be significantly less (P less than 0.05) than between-subject variance; thus, uniqueness of individual dogs was reliably revealed. This technique also was used to demonstrated breed differences for acid-base and blood gas characteristics. | [
-0.013162986375391483,
0.018272943794727325,
-0.009768383577466011,
0.014511876739561558,
-0.002316197846084833,
-0.01644829660654068,
0.08756480365991592,
0.03507951274514198,
-0.017136765643954277,
0.015445726923644543,
0.04277779534459114,
-0.10072476416826248,
-0.0007798980223014951,
0.0399201400578022,
-0.03675462678074837,
-0.038825713098049164,
0.01100524328649044,
0.018905790522694588,
-0.06508047878742218,
-0.008914068341255188,
-0.02239621803164482,
-0.011309045366942883,
0.030662788078188896,
-0.038590528070926666,
0.04444156587123871,
0.010307101532816887,
0.06309735774993896,
0.007889577187597752,
-0.017929894849658012,
-0.14038097858428955,
0.0014606021577492356,
-0.01953303813934326,
0.000040137751057045534,
0.00634078960865736,
-0.009341501630842686,
0.029747821390628815,
0.0016230229521170259,
-0.018484288826584816,
-0.039288315922021866,
0.03738071769475937,
0.08718030154705048,
0.019450876861810684,
-0.046958886086940765,
0.035243626683950424,
-0.010772820562124252,
0.018919823691248894,
-0.027143999934196472,
0.009244117885828018,
0.06596747040748596,
-0.006756615824997425,
-0.020934730768203735,
-0.017588526010513306,
0.036010727286338806,
0.022648131474852562,
0.00024199645849876106,
-0.028861500322818756,
0.0020674525294452906,
-0.02226945571601391,
0.029291076585650444,
-0.03837035223841667,
0.004007687326520681,
0.09091728180646896,
-0.11433877795934677,
0.056785404682159424,
0.006360158324241638,
-0.015602710656821728,
-0.05453093722462654,
-0.06748645007610321,
0.08339891582727432,
-0.002220484660938382,
0.006613276898860931,
0.00021538279543165118,
0.029538653790950775,
-0.011158999986946583,
-0.0072707319632172585,
-0.004695225972682238,
0.03486267849802971,
-0.011573884636163712,
0.014061592519283295,
-0.012238623574376106,
-0.03749644011259079,
0.013423885218799114,
0.005775117315351963,
-0.0038665763568133116,
0.007667352445423603,
-0.037845440208911896,
0.016280444338917732,
-0.016093721613287926,
-0.05607488006353378,
0.032855916768312454,
-0.01825878396630287,
-0.05247657373547554,
-0.0685419887304306,
-0.0017505708383396268,
-0.02775091491639614,
0.0154744703322649,
0.03759482130408287,
-0.008120403625071049,
0.038649704307317734,
0.4207817316055298,
-0.01612112857401371,
-0.02659042924642563,
-0.04449417069554329,
-0.030480975285172462,
0.04965495690703392,
-0.007196636404842138,
-0.04411652684211731,
-0.008753811940550804,
-0.047739461064338684,
0.0340409055352211,
0.04726811870932579,
0.01695011928677559,
0.028299467638134956,
-0.040227845311164856,
-0.016208600252866745,
0.03718224912881851,
0.004375997465103865,
0.029666174203157425,
0.030947456136345863,
0.06204519048333168,
-0.019175875931978226,
-0.02699635550379753,
-0.005313342437148094,
-0.030082115903496742,
0.11303877830505371,
-0.006076645106077194,
0.009846784174442291,
0.053774334490299225,
0.036134153604507446,
0.02003660425543785,
0.021215373650193214,
-0.006000110413879156,
-0.03215966746211052,
-0.06762339174747467,
-0.03510443866252899,
-0.020827436819672585,
-0.0012482724850997329,
0.014566173776984215,
0.022295117378234863,
0.00480315089225769,
-0.04661386087536812,
-0.046819206327199936,
-0.049138400703668594,
-0.04951751232147217,
-0.10340817272663116,
0.11241788417100906,
0.010182741098105907,
-0.0019879958126693964,
-0.05022561550140381,
-0.03576315566897392,
-0.02474651113152504,
-0.06878476589918137,
0.007587946020066738,
0.04832076653838158,
-0.0001774696575012058,
0.047995295375585556,
-0.05897581949830055,
0.021275479346513748,
0.016818061470985413,
-0.008831198327243328,
0.01995186135172844,
-0.0491163432598114,
-0.02113642357289791,
0.050937019288539886,
0.0022867899388074875,
-0.011044054292142391,
-0.03955757990479469,
-0.05455635488033295,
0.07111460715532303,
0.01733005978167057,
0.03578246384859085,
-0.0032481851521879435,
-0.008729910477995872,
-0.018770553171634674,
-0.033527225255966187,
0.017258044332265854,
-0.007762788329273462,
0.030177831649780273,
0.018217846751213074,
0.012156139127910137,
0.0867065042257309,
0.037306249141693115,
-0.01900077797472477,
0.011037502437829971,
0.018549839034676552,
-0.01990918442606926,
-0.045837029814720154,
-0.04081149771809578,
0.013318653218448162,
0.09646975994110107,
-0.020175334066152573,
-0.033122312277555466,
-0.0255194753408432,
-0.029085583984851837,
0.0052297827787697315,
0.02178952284157276,
-0.026959901675581932,
0.03360830247402191,
-0.016640642657876015,
0.043218135833740234,
0.08018192648887634,
-0.027771933004260063,
0.0028421294409781694,
0.027496295049786568,
0.1287841945886612,
0.0815507248044014,
0.03433976694941521,
-0.04295392334461212,
0.027768122032284737,
-0.013488101772964,
0.03562552481889725,
0.03821410611271858,
-0.07027216255664825,
0.03293309360742569,
-0.008198411203920841,
0.024677680805325508,
0.0014352425932884216,
-0.029704438522458076,
0.042966146022081375,
-0.000718643597792834,
0.05957568436861038,
-0.13059957325458527,
-0.002797620138153434,
-0.3351881206035614,
-0.02740428037941456,
-0.021694406867027283,
-0.01020926795899868,
0.06838145107030869,
-0.035529445856809616,
0.06461402028799057,
-0.03556099906563759,
0.009254910983145237,
-0.02892974577844143,
-0.00013339199358597398,
0.03242034092545509,
0.012738315388560295,
-0.035355664789676666,
0.04256926476955414,
0.10344687849283218,
0.005461206194013357,
-0.054595936089754105,
0.048874206840991974,
0.04942803084850311,
0.06421201676130295,
-0.023353131487965584,
-0.014911509118974209,
0.0021427529864013195,
0.04004641994833946,
-0.013875279575586319,
0.13589061796665192,
-0.08156267553567886,
0.013147478923201561,
-0.027358748018741608,
0.00380676775239408,
-0.018030568957328796,
-0.010229703038930893,
-0.08172616362571716,
-0.019771605730056763,
-0.037987831979990005,
-0.007675555068999529,
0.008438339456915855,
-0.021188663318753242,
-0.033125489950180054,
-0.06461575627326965,
-0.005792370531708002,
-0.05585569888353348,
-0.05644545704126358,
-0.05599215626716614,
0.02950596995651722,
-0.005449858959764242,
-0.01183716394007206,
-0.05064278841018677,
-0.021643666550517082,
0.0374862402677536,
0.023187199607491493,
-0.045537322759628296,
-0.014913763850927353,
-0.005104872398078442,
-0.04170290008187294,
-0.03857230395078659,
0.016016896814107895,
-0.0592937171459198,
-0.01758813112974167,
0.04913053289055824,
-0.05091608688235283,
0.05972915515303612,
0.0023433947935700417,
0.010179566219449043,
0.025236230343580246,
0.03319314867258072,
0.031536925584077835,
0.044207848608493805,
0.012154731899499893,
0.012384029105305672,
0.08990668505430222,
-0.0003049677761737257,
-0.024209944531321526,
0.043042413890361786,
-0.00902167521417141,
0.011578807607293129,
-0.039480745792388916,
-0.059097081422805786,
0.000013099302123009693,
0.015670763328671455,
-0.0616481676697731,
0.06840416043996811,
-0.01795378141105175,
-0.00256149610504508,
0.05742263421416283,
0.03246741741895676,
-0.030282577499747276,
0.009381067007780075,
-0.027427690103650093,
0.01368302945047617,
-0.007414041552692652,
-0.0341305173933506,
-0.042660459876060486,
0.019693825393915176,
0.01998393051326275,
-0.28539732098579407,
-0.015659058466553688,
0.03567203879356384,
-0.00036772387102246284,
-0.033120714128017426,
-0.03203703835606575,
-0.028324292972683907,
-0.04072403535246849,
0.021671976894140244,
-0.0053728255443274975,
-0.012012282386422157,
0.04144228622317314,
0.02986587956547737,
0.03580069914460182,
-0.012395590543746948,
0.051066383719444275,
0.03971373289823532,
-0.02176302671432495,
-0.00334944948554039,
0.017343949526548386,
0.010847752913832664,
-0.05678603798151016,
0.14240321516990662,
0.011026369407773018,
-0.04374360665678978,
0.027419088408350945,
0.011815750040113926,
0.02427736110985279,
-0.00040007487405091524,
-0.035489682108163834,
0.06691645085811615,
-0.014844448305666447,
0.038360483944416046,
0.0023687761276960373,
-0.003401457564905286,
0.045719318091869354,
-0.008421238511800766,
0.037767164409160614,
0.03266223147511482,
-0.004616104066371918,
0.039633676409721375,
0.002912415424361825,
-0.020382720977067947,
-0.06549111008644104,
0.07187047600746155,
0.009422737173736095,
-0.01802467554807663,
-0.025074439123272896,
0.028476154431700706,
-0.044575974345207214,
-0.06172395125031471,
-0.060741979628801346,
0.03596646711230278,
-0.014543656259775162,
0.020822307094931602,
0.008214893750846386,
0.028623947873711586,
-0.02528286539018154,
0.027751700952649117,
-0.04534813016653061,
0.01650363579392433,
-0.02539929375052452,
-0.026294052600860596,
0.10363257676362991,
0.04325653240084648
] |
PMID:25032 | Type-specific pneumococcal respiratory disease in the elderly and chronically III. | Type-specific pneumococcal respiratory disease was studied in a chronic-disease hospital during a 27-month period. Isolates from 50 patients with pneumonia and from 24 patients with chronic bronchitis were available for typing. Vaccine types were isolated from 74 per cent of patients with pneumonia and from 42 per cent of patients with chronic bronchitis. Pneumococcal types isolated from 5 of 8 patients with bacteremia and from 6 of 9 patients who died were also included in the vaccine. The data suggest that, theoretically, closed populations of elderly and chronically ill patients would benefit from vaccination in an attempt to control pneumococcal pneumonia. Less clear is the potential role vaccination in patients with chronic bronchitis. | Type-specific pneumococcal respiratory disease in the elderly and chronically III. Type-specific pneumococcal respiratory disease was studied in a chronic-disease hospital during a 27-month period. Isolates from 50 patients with pneumonia and from 24 patients with chronic bronchitis were available for typing. Vaccine types were isolated from 74 per cent of patients with pneumonia and from 42 per cent of patients with chronic bronchitis. Pneumococcal types isolated from 5 of 8 patients with bacteremia and from 6 of 9 patients who died were also included in the vaccine. The data suggest that, theoretically, closed populations of elderly and chronically ill patients would benefit from vaccination in an attempt to control pneumococcal pneumonia. Less clear is the potential role vaccination in patients with chronic bronchitis. | [
-0.05008387565612793,
0.03617960214614868,
-0.042945556342601776,
0.0190837811678648,
0.03639935702085495,
-0.011502115987241268,
0.016194717958569527,
-0.005155143328011036,
-0.06836830824613571,
0.05000808462500572,
-0.0005676590953953564,
-0.04558687284588814,
0.007721835747361183,
0.025102615356445312,
0.01772220991551876,
0.009348005056381226,
-0.004383973311632872,
-0.040517259389162064,
-0.03381786122918129,
0.05749004706740379,
-0.03228947892785072,
0.021251192316412926,
0.010243333876132965,
-0.0557514913380146,
0.027569206431508064,
0.022355929017066956,
0.043152883648872375,
-0.06428741663694382,
-0.00711118383333087,
-0.15606747567653656,
-0.03222605958580971,
-0.009425089694559574,
-0.02303195185959339,
-0.010194317437708378,
-0.05269360542297363,
-0.053805142641067505,
0.01558209490031004,
0.01425901334732771,
0.006592394318431616,
0.04112061858177185,
0.046627476811409,
0.012791628949344158,
0.0388210155069828,
-0.022579174488782883,
-0.02672657184302807,
-0.024647926911711693,
-0.10960369557142258,
0.008597939275205135,
0.0944935530424118,
0.020238975062966347,
-0.01408584788441658,
0.03395981714129448,
-0.01820657216012478,
0.0917530432343483,
-0.045952923595905304,
-0.04432383179664612,
-0.015422186814248562,
0.027535991743206978,
-0.03887375071644783,
-0.03494325280189514,
-0.013642915524542332,
0.012026814743876457,
-0.10024911910295486,
0.07785894721746445,
-0.023128898814320564,
0.08113056421279907,
0.005806869361549616,
-0.034569889307022095,
0.030671415850520134,
0.013216876424849033,
-0.06723582744598389,
0.04727685824036598,
-0.052569806575775146,
0.08308415859937668,
0.0037513559218496084,
0.02909594029188156,
-0.04937780275940895,
-0.04375071078538895,
0.04194585978984833,
0.028153514489531517,
0.0011883446713909507,
0.0673891007900238,
-0.020025379955768585,
-0.01438851933926344,
-0.06779513508081436,
-0.014283053576946259,
0.03555328771471977,
-0.06281092762947083,
-0.036326367408037186,
-0.0017979071708396077,
0.023360269144177437,
0.05440199002623558,
-0.05037585273385048,
0.06484600156545639,
-0.03498145192861557,
0.003400527173653245,
0.07919643819332123,
0.013563202694058418,
-0.02229909412562847,
0.3961907923221588,
-0.019993377849459648,
-0.06436572223901749,
0.003798858029767871,
0.002740203868597746,
0.05609138682484627,
0.019879790022969246,
0.032141391187906265,
-0.05443470552563667,
0.009354254230856895,
0.005623998586088419,
-0.06624993681907654,
0.02452690526843071,
0.004771220963448286,
-0.008143129758536816,
-0.07935452461242676,
0.030925774946808815,
0.04411729425191879,
0.05445544421672821,
-0.016792340204119682,
0.01460986491292715,
-0.052907463163137436,
-0.054149433970451355,
0.029868237674236298,
-0.04746752604842186,
0.05296926945447922,
0.026634706184267998,
-0.015438513830304146,
0.06811556965112686,
0.0314534455537796,
0.020766614004969597,
0.06651187688112259,
0.029667751863598824,
0.019058378413319588,
-0.011212335899472237,
-0.026408109813928604,
-0.01680561527609825,
-0.019680717960000038,
-0.021620985120534897,
0.03211251273751259,
0.00032852331059984863,
-0.021759893745183945,
-0.05533032491803169,
-0.007941325195133686,
-0.035994261503219604,
-0.08394565433263779,
0.06311991065740585,
-0.08362443000078201,
0.03415442258119583,
-0.004495266359299421,
0.014293578453361988,
0.007386862765997648,
0.10282216966152191,
-0.05619540810585022,
0.01300336979329586,
0.04472596198320389,
0.030224096029996872,
0.0271921269595623,
-0.022543570026755333,
-0.01766151934862137,
-0.04505051299929619,
-0.06376566737890244,
-0.05859645456075668,
-0.013179083354771137,
0.0612245537340641,
-0.052143678069114685,
-0.013002614490687847,
-0.015885667875409126,
0.01813342794775963,
0.0008809686987660825,
0.002027452690526843,
0.023611363023519516,
0.02956157736480236,
0.03518370911478996,
0.00794332753866911,
0.0084397466853261,
0.06488186120986938,
-0.06214941293001175,
-0.03161758929491043,
0.0014598070411011577,
-0.003882819088175893,
-0.04046229273080826,
0.022444551810622215,
0.002204173244535923,
0.005510908551514149,
0.0046066478826105595,
0.010889386758208275,
-0.01708713173866272,
0.029408087953925133,
0.048173222690820694,
-0.03079674392938614,
0.03950468823313713,
-0.013596085831522942,
-0.05218200385570526,
-0.039423611015081406,
0.0306385550647974,
-0.007254835683852434,
-0.06669704616069794,
-0.027121610939502716,
0.06547373533248901,
-0.05232878029346466,
0.03137296065688133,
0.005343014374375343,
-0.0265145692974329,
-0.01952344737946987,
0.013127852231264114,
-0.020360464230179787,
0.018915344029664993,
-0.02347966656088829,
0.044122420251369476,
-0.021949663758277893,
-0.04571819305419922,
0.022479884326457977,
-0.02369886077940464,
0.018531158566474915,
-0.04200350120663643,
0.033695727586746216,
-0.017765866592526436,
-0.021567238494753838,
0.05141013115644455,
0.01671745441854,
0.012692692689597607,
0.04294552654027939,
-0.010979210026562214,
-0.2980506122112274,
-0.030840858817100525,
0.024266231805086136,
-0.031245270743966103,
0.006523770280182362,
-0.02339380607008934,
-0.03457482531666756,
0.05579202622175217,
-0.021730253472924232,
-0.02685258723795414,
0.05267850309610367,
0.04185374826192856,
-0.0957699865102768,
-0.0245957188308239,
-0.004577564541250467,
0.1321423202753067,
0.0394970066845417,
-0.06682288646697998,
-0.0545952282845974,
-0.023428218439221382,
0.04548855498433113,
-0.0527343675494194,
0.023649010807275772,
0.009403659030795097,
0.04102699086070061,
-0.05418222397565842,
0.1348806917667389,
-0.06389512121677399,
0.04225967079401016,
0.015875622630119324,
0.015178985893726349,
-0.011988203041255474,
0.0041556027717888355,
-0.03482186049222946,
0.026495512574911118,
-0.05212617293000221,
-0.035536326467990875,
0.017233483493328094,
-0.014469108544290066,
0.03307800740003586,
-0.04728953167796135,
0.003591112559661269,
-0.029744384810328484,
-0.02861189655959606,
-0.003788052126765251,
-0.03250749409198761,
0.051989395171403885,
0.06386362016201019,
-0.05140044167637825,
0.01651151292026043,
0.018603025004267693,
-0.013364000245928764,
-0.004058916587382555,
0.04716481268405914,
-0.0014739511534571648,
0.021127993240952492,
-0.0014836881309747696,
-0.03105849213898182,
-0.04476069286465645,
0.03329896181821823,
-0.019979111850261688,
-0.012280268594622612,
0.04238630831241608,
0.0030476527754217386,
-0.045713577419519424,
-0.051151927560567856,
-0.0007063915836624801,
-0.018331559374928474,
0.029407093301415443,
0.029530450701713562,
-0.02528359554708004,
0.07560266554355621,
-0.055873073637485504,
-0.0601920448243618,
0.025763148441910744,
0.04211552441120148,
-0.0011604103492572904,
-0.06056448444724083,
-0.02499964088201523,
0.012582299299538136,
0.05078680440783501,
-0.04271965101361275,
0.02899738773703575,
0.07251080870628357,
0.00842925626784563,
0.0049292235635221004,
-0.019043512642383575,
0.042760152369737625,
-0.030473167076706886,
-0.030171483755111694,
0.002941759070381522,
-0.006870192475616932,
-0.009923652745783329,
-0.04681938886642456,
0.01440408919006586,
0.0498531199991703,
-0.2732657492160797,
0.029567983001470566,
0.03718751296401024,
0.040081217885017395,
0.013843711465597153,
0.05422782525420189,
-0.03644229844212532,
-0.05731935054063797,
-0.03296960890293121,
0.010235740803182125,
0.08786974102258682,
0.021875180304050446,
0.08527781069278717,
0.011842574924230576,
-0.018731122836470604,
-0.000829907541628927,
-0.0038233590312302113,
-0.023603472858667374,
0.06904271990060806,
-0.03363332152366638,
0.011871343478560448,
-0.02722861059010029,
0.11945591121912003,
0.015413869172334671,
-0.0010044267401099205,
-0.0012210817076265812,
0.03225820139050484,
0.06589453667402267,
-0.00820048525929451,
0.030146488919854164,
0.07811900973320007,
-0.07465513050556183,
0.10652806609869003,
0.020349446684122086,
0.03322678804397583,
-0.017158357426524162,
0.006574488710612059,
0.049025606364011765,
-0.007948522455990314,
-0.013472451828420162,
-0.04048764333128929,
-0.024557096883654594,
-0.004342509433627129,
-0.03135199472308159,
0.07394568622112274,
-0.012158364988863468,
-0.02400890178978443,
-0.08098283410072327,
-0.0463823601603508,
0.0003061933384742588,
0.028637627139687538,
0.00926548708230257,
0.03913096711039543,
0.051668014377355576,
0.08535809814929962,
-0.06491337716579437,
0.027536222711205482,
0.0006280165980570018,
0.00021136784926056862,
0.01895918883383274,
0.022411586716771126,
-0.021197767928242683,
-0.10725521296262741,
0.08081978559494019,
0.06140758469700813
] |
PMID:25034 | Subacute thyroiditis with increased serum alkaline phosphatase. | Three patients had subacute thyroiditis and elevated serum alkaline phosphatase, presumably related to the thyroiditis. Concomitant elevation of the serum gamma-glutamyl transferase suggested that the alkaline phosphatase was of hepatic origin. The elevation of the serum alkaline phosphatase could not be definitely related to the degree or duration of elevation of the serum thyroxine. The combination of elevated serum alkaline phosphatase with the systemic symptoms of subacute thyroiditis may obscure the diagnosis, especially if patients have little or no neck pain. | Subacute thyroiditis with increased serum alkaline phosphatase. Three patients had subacute thyroiditis and elevated serum alkaline phosphatase, presumably related to the thyroiditis. Concomitant elevation of the serum gamma-glutamyl transferase suggested that the alkaline phosphatase was of hepatic origin. The elevation of the serum alkaline phosphatase could not be definitely related to the degree or duration of elevation of the serum thyroxine. The combination of elevated serum alkaline phosphatase with the systemic symptoms of subacute thyroiditis may obscure the diagnosis, especially if patients have little or no neck pain. | [
0.03309740498661995,
0.014071973972022533,
0.023121684789657593,
0.051069051027297974,
0.0009047000203281641,
-0.071443572640419,
0.11623267084360123,
0.04933338984847069,
-0.07288377732038498,
-0.04870416596531868,
-0.0013045964296907187,
-0.09250123798847198,
0.02948504313826561,
0.026165254414081573,
-0.018948085606098175,
-0.043049827218055725,
0.02908388338983059,
-0.07033172249794006,
-0.08053537458181381,
0.028608985245227814,
-0.059421904385089874,
0.03693941980600357,
0.04164007306098938,
-0.04653483256697655,
0.06130385398864746,
0.04824090376496315,
-0.0043515912257134914,
0.012242002412676811,
-0.04489682614803314,
-0.15131862461566925,
-0.014310088008642197,
-0.026533614844083786,
-0.044144224375486374,
-0.025423983111977577,
0.021394653245806694,
0.04932926595211029,
-0.057806480675935745,
0.03947867453098297,
0.008836477063596249,
0.02481519989669323,
0.003735616570338607,
-0.02186940237879753,
-0.048178672790527344,
0.04009278491139412,
-0.022687667980790138,
0.006362014450132847,
-0.041752394288778305,
0.06298144161701202,
0.11092746257781982,
0.01848585531115532,
0.017734937369823456,
0.02822546288371086,
0.04066186770796776,
0.10153397917747498,
-0.003194758901372552,
-0.007041222881525755,
-0.04181777685880661,
0.0350741483271122,
0.004246705677360296,
-0.015753036364912987,
-0.023272937163710594,
0.024419911205768585,
-0.08714862912893295,
0.057165663689374924,
0.03708404675126076,
0.043555933982133865,
-0.001634400337934494,
-0.028585292398929596,
-0.0013746096519753337,
0.02163911610841751,
-0.025976695120334625,
-0.00018997916777152568,
-0.02298806980252266,
0.023014331236481667,
0.022990895435214043,
0.004447054583579302,
-0.003104499774053693,
-0.0018424085574224591,
0.011714077554643154,
0.03614437207579613,
0.01250218041241169,
0.04364452138543129,
-0.003778147744014859,
-0.01993349939584732,
-0.02248447574675083,
-0.04443880543112755,
0.007181648164987564,
-0.047746770083904266,
-0.00025559531059116125,
0.030073130503296852,
-0.004428388550877571,
-0.1003178209066391,
0.013714672066271305,
0.004044038243591785,
-0.012551447376608849,
0.07030463218688965,
0.046966712921857834,
0.03821980580687523,
-0.015749499201774597,
0.3329501748085022,
-0.04825460910797119,
-0.012214044108986855,
-0.05910904332995415,
0.01303891185671091,
-0.04226170480251312,
-0.02474655956029892,
0.009020939469337463,
-0.044726256281137466,
-0.04319848120212555,
0.015463328920304775,
0.019821206107735634,
0.020146094262599945,
0.020707054063677788,
-0.09390443563461304,
-0.020022636279463768,
0.030725328251719475,
0.04588444158434868,
0.0047219134867191315,
-0.039745770394802094,
0.0009755328646861017,
-0.01272389106452465,
-0.010061441920697689,
0.0038690282963216305,
-0.09129969775676727,
-0.018176140263676643,
0.0915149673819542,
0.011064332909882069,
0.07944393157958984,
0.0642547458410263,
-0.01596495881676674,
0.04702972248196602,
-0.018941018730401993,
0.01815006509423256,
-0.022767582908272743,
-0.030710920691490173,
-0.004309786017984152,
0.006403501145541668,
-0.04274352639913559,
0.050039809197187424,
0.05937117338180542,
0.021557101979851723,
-0.09946822375059128,
-0.09324496239423752,
-0.002919344697147608,
-0.0013427595840767026,
0.09911651164293289,
-0.08529799431562424,
0.07954173535108566,
0.008322897367179394,
-0.07599847763776779,
0.005901785101741552,
0.004170702304691076,
-0.014844253659248352,
0.05256475508213043,
-0.012921581044793129,
-0.013311979360878468,
-0.02359158918261528,
0.0027334485203027725,
0.003205350134521723,
-0.05968184769153595,
-0.04905831813812256,
0.009108033031225204,
-0.030446235090494156,
0.0003091156540904194,
0.041638780385255814,
0.006871266756206751,
0.0016688548494130373,
-0.013323306106030941,
-0.016791226342320442,
0.008894568309187889,
-0.006939751096069813,
0.04439713433384895,
0.004478175193071365,
-0.006086096167564392,
0.014426319859921932,
0.03246954083442688,
-0.003383360803127289,
0.02647067978978157,
0.008754545822739601,
-0.05835871398448944,
0.05347267910838127,
0.009535652585327625,
-0.043021176010370255,
0.0664951503276825,
0.022316835820674896,
-0.02478301152586937,
0.007026165258139372,
0.007593280635774136,
0.00811716727912426,
-0.0016751247458159924,
-0.07795793563127518,
-0.015925979241728783,
-0.06531599909067154,
-0.044885654002428055,
-0.010569600388407707,
-0.000656549702398479,
-0.02827960066497326,
0.035385262221097946,
0.06146536394953728,
0.0029782915953546762,
0.1035335585474968,
0.04571064934134483,
0.0025808163918554783,
0.011550262570381165,
-0.015755588188767433,
0.004095394629985094,
0.009043454192578793,
0.047535572201013565,
0.07903345674276352,
0.02848312258720398,
-0.005256881006062031,
-0.010214366018772125,
-0.07318150252103806,
0.021375438198447227,
-0.04995308071374893,
0.017595361918210983,
-0.03267635032534599,
-0.025187620893120766,
0.01590929552912712,
0.030954981222748756,
0.04661031439900398,
-0.049989305436611176,
-0.039575062692165375,
-0.2669532895088196,
-0.0010083564557135105,
0.025223523378372192,
0.007885986007750034,
0.0023300459142774343,
-0.048931438475847244,
0.011332494206726551,
0.04598289728164673,
-0.036748457700014114,
-0.026578441262245178,
0.06561434268951416,
0.09708219766616821,
-0.05281518027186394,
-0.06997870653867722,
0.0003419639833737165,
0.057148803025484085,
0.033155035227537155,
0.010733844712376595,
0.06430647522211075,
0.00969511829316616,
0.014659849926829338,
-0.02968193031847477,
0.021307701244950294,
0.06736239790916443,
0.012578336521983147,
0.03199001029133797,
0.07935645431280136,
-0.007025412283837795,
-0.05492491275072098,
-0.08391030877828598,
0.008546999655663967,
-0.012067942880094051,
0.01423207949846983,
-0.08274385333061218,
0.021278465166687965,
-0.04031050205230713,
-0.06332197785377502,
-0.0030295869801193476,
-0.058386482298374176,
-0.007982064969837666,
-0.09049298614263535,
-0.04214520379900932,
-0.03911087289452553,
-0.03465013578534126,
0.01312110386788845,
0.013624987564980984,
-0.0020414511673152447,
0.038981009274721146,
0.0272169541567564,
0.04194687306880951,
0.017460770905017853,
0.05513591691851616,
0.017889847978949547,
-0.03473701700568199,
0.022254111245274544,
-0.016431432217359543,
-0.09967818856239319,
0.02957131527364254,
-0.019855765625834465,
-0.005075224209576845,
-0.0227931160479784,
0.02148032747209072,
0.01293619442731142,
0.030815793201327324,
0.008968510664999485,
-0.02004712074995041,
0.00256255641579628,
0.02418953739106655,
-0.00872181449085474,
0.05073622614145279,
-0.009613985195755959,
0.09495341032743454,
-0.02714972011744976,
-0.015860026702284813,
-0.009941975586116314,
0.04130198433995247,
-0.02041562646627426,
-0.05024731159210205,
-0.03907550126314163,
0.01247004047036171,
0.0027385076973587275,
-0.09248204529285431,
0.020498162135481834,
0.04536781087517738,
-0.03477894887328148,
0.053800735622644424,
0.012074040248990059,
0.029441295191645622,
-0.007823805324733257,
0.034693703055381775,
-0.010832083411514759,
0.018875746056437492,
-0.05259549617767334,
-0.043855179101228714,
0.0024547239299863577,
-0.006043788976967335,
-0.3207419216632843,
0.00804237648844719,
0.024622345343232155,
-0.08894576877355576,
-0.04219713434576988,
0.042641885578632355,
-0.006337933707982302,
-0.00016699355910532176,
-0.05841275304555893,
0.052450839430093765,
0.05023373290896416,
0.005664885509759188,
0.07386325299739838,
0.0042868019081652164,
-0.07126396149396896,
-0.023685723543167114,
0.0445442870259285,
0.011971275322139263,
0.027616385370492935,
-0.03545825928449631,
0.00832801777869463,
-0.029051633551716805,
0.1950116604566574,
0.00912274606525898,
0.032101429998874664,
0.060469262301921844,
0.007144922856241465,
0.037656959146261215,
0.009520196355879307,
-0.04591333866119385,
0.013005335815250874,
0.023892201483249664,
0.07008061558008194,
-0.026240531355142593,
0.009591233916580677,
0.06450039148330688,
0.007653567939996719,
-0.003428952768445015,
-0.05072568729519844,
0.010337204672396183,
0.03700917959213257,
0.0687679871916771,
-0.08611619472503662,
-0.08058324456214905,
0.08858467638492584,
-0.03473064675927162,
-0.004057764541357756,
-0.03253015875816345,
0.009600942954421043,
-0.0076764714904129505,
0.006390667520463467,
-0.021089565008878708,
0.022234424948692322,
0.10756342113018036,
0.010871732607483864,
-0.038717132061719894,
-0.017865722998976707,
-0.004598076455295086,
-0.006409862078726292,
-0.03469907492399216,
0.028158079832792282,
-0.02569686248898506,
-0.04164883494377136,
0.08053893595933914,
0.020393379032611847
] |
PMID:25035 | Clinical and biochemical heterogeneity of depressive disorders. | Indirect evidence, mostly pharmacologic, has suggested a role for brain neurotransmitter amines such as norepinephrine in the production of depression or mania. Clinical investigations have supported this concept but also indicate that depression is probably a biochemically heterogeneous group of illnesses. There may be a clinically, biochemically, and pharmacologically definable subtype of depression in which there is a disorder of norepinephrine metabolism or disposition in brain. I review here the experimental data from which this hypothesis is derived. | Clinical and biochemical heterogeneity of depressive disorders. Indirect evidence, mostly pharmacologic, has suggested a role for brain neurotransmitter amines such as norepinephrine in the production of depression or mania. Clinical investigations have supported this concept but also indicate that depression is probably a biochemically heterogeneous group of illnesses. There may be a clinically, biochemically, and pharmacologically definable subtype of depression in which there is a disorder of norepinephrine metabolism or disposition in brain. I review here the experimental data from which this hypothesis is derived. | [
0.03024064376950264,
0.0031528521794825792,
-0.0156710147857666,
-0.011969507671892643,
0.014414268545806408,
0.034792665392160416,
0.10049392282962799,
0.005296580493450165,
0.03088100254535675,
-0.06378120929002762,
0.04741841182112694,
-0.014022818766534328,
-0.003674654522910714,
0.07665993273258209,
-0.012760907411575317,
0.016080433502793312,
0.044777095317840576,
-0.006728778593242168,
-0.04613398760557175,
-0.004292360506951809,
-0.0018834302900359035,
-0.017008101567626,
0.0294045377522707,
0.0371747650206089,
0.0038443265948444605,
0.0014485857682302594,
-0.007553936913609505,
0.0023709628731012344,
-0.06750412285327911,
-0.13350950181484222,
0.038343217223882675,
0.04339813441038132,
0.050118960440158844,
-0.019738493487238884,
0.051752399653196335,
0.07018335908651352,
-0.04113401472568512,
0.049734968692064285,
-0.0133728738874197,
0.052255358546972275,
0.0157433170825243,
0.0026681784074753523,
-0.006035808939486742,
0.013502500019967556,
0.0365624874830246,
0.004898601211607456,
-0.06141382455825806,
-0.006230394821614027,
0.03436436131596565,
-0.020023902878165245,
-0.011670386418700218,
0.026146480813622475,
0.010773385874927044,
0.0987047553062439,
-0.006475736852735281,
0.017217472195625305,
-0.010508257895708084,
0.006169698666781187,
-0.026993805542588234,
0.06259270757436752,
-0.01997852325439453,
0.050176024436950684,
-0.1410031020641327,
0.02889203652739525,
0.12606841325759888,
-0.03717787563800812,
-0.087517648935318,
-0.012138939462602139,
-0.0002873191551771015,
0.012759891338646412,
-0.06155513972043991,
-0.016430875286459923,
0.027218542993068695,
-0.04439940303564072,
-0.0012238655472174287,
0.0014046046417206526,
0.023189490661025047,
-0.04017762094736099,
0.0593283548951149,
-0.031239250674843788,
0.06814663857221603,
0.03631718456745148,
0.007050899788737297,
-0.04897254705429077,
0.007013935595750809,
-0.03240238130092621,
-0.036561269313097,
-0.036562636494636536,
-0.004401163663715124,
-0.01499621756374836,
0.00168694113381207,
-0.05310872197151184,
0.02890786901116371,
0.013375962153077126,
-0.010519408620893955,
0.012428762391209602,
0.01560414582490921,
-0.03801960125565529,
0.019543474540114403,
0.3984481692314148,
-0.012574221007525921,
0.020644478499889374,
0.010640361346304417,
0.026584314182400703,
-0.027060020714998245,
0.01076348964124918,
-0.0599607415497303,
-0.09734292328357697,
-0.00500353891402483,
0.012923847883939743,
-0.0019201545510441065,
0.02200295589864254,
0.05716210976243019,
-0.10072141140699387,
-0.055690884590148926,
-0.044509515166282654,
0.03450872376561165,
-0.03298896178603172,
-0.0016758915735408664,
0.006931480020284653,
-0.018492253497242928,
0.0012055030092597008,
0.008287865668535233,
-0.00918838381767273,
0.020730316638946533,
-0.028172679245471954,
-0.04481525346636772,
0.06631145626306534,
0.06925659626722336,
-0.05428527295589447,
0.06129961460828781,
0.040168896317481995,
-0.024196652695536613,
0.021342528983950615,
0.02805383689701557,
-0.019643142819404602,
0.049374934285879135,
-0.01690680719912052,
0.03604329749941826,
0.0017963011050596833,
0.0028311400674283504,
-0.04020444676280022,
-0.008282274939119816,
-0.04847480356693268,
-0.07616355270147324,
0.12531371414661407,
0.015446365810930729,
-0.013889404945075512,
0.011026935651898384,
0.023496702313423157,
-0.023923182860016823,
0.04718547314405441,
-0.0319596529006958,
0.05861556529998779,
-0.04026292636990547,
0.055241040885448456,
0.0035261493176221848,
-0.05917984992265701,
-0.0317559540271759,
0.00915008969604969,
-0.0039035945665091276,
-0.004230901133269072,
-0.013768111355602741,
0.10156408697366714,
0.01823420450091362,
0.020048566162586212,
-0.04527802765369415,
0.002939885016530752,
-0.004451878368854523,
0.006498287431895733,
0.015029145404696465,
0.005826986860483885,
0.03848182410001755,
-0.020588736981153488,
-0.09752565622329712,
-0.017855919897556305,
0.026377011090517044,
0.0367252416908741,
-0.009162897244095802,
-0.02557177096605301,
0.066676564514637,
-0.023203982040286064,
0.001200609840452671,
0.026567429304122925,
0.05205196887254715,
-0.018906280398368835,
0.00968841090798378,
-0.008213990367949009,
-0.01217272412031889,
0.028143787756562233,
-0.05993993207812309,
-0.00939200259745121,
0.01949862204492092,
-0.010374785400927067,
-0.022428564727306366,
-0.04868397116661072,
0.01155630499124527,
-0.016929663717746735,
-0.021893205121159554,
0.03390198573470116,
0.015825346112251282,
0.06896518915891647,
0.012144484557211399,
0.054129038006067276,
0.05934341624379158,
-0.006933488417416811,
0.021368617191910744,
-0.00439792824909091,
0.06302561610937119,
-0.030348937958478928,
0.006330091040581465,
-0.013204643502831459,
-0.008572567254304886,
0.02140587754547596,
-0.05077092722058296,
0.028740083798766136,
-0.0035740032326430082,
0.05841401591897011,
0.13731561601161957,
0.0029648328199982643,
-0.041500866413116455,
-0.06683827936649323,
-0.02095642499625683,
-0.2527102530002594,
-0.0748075395822525,
0.00519161531701684,
0.0043722642585635185,
-0.02550111897289753,
0.0030446944292634726,
-0.006385268643498421,
-0.017354914918541908,
-0.025194400921463966,
0.06747715920209885,
0.02238471619784832,
0.043635234236717224,
-0.029175149276852608,
-0.03536110743880272,
0.003614965360611677,
-0.0045148711651563644,
0.049847763031721115,
-0.05405102297663689,
-0.024802807718515396,
0.029642218723893166,
-0.014037048444151878,
0.002512826817110181,
-0.005705700255930424,
-0.016952667385339737,
0.025826770812273026,
-0.055610109120607376,
0.122006356716156,
0.0042373728938400745,
0.004927228204905987,
-0.003776912111788988,
-0.03475464880466461,
0.07384540140628815,
-0.019512973725795746,
-0.11421726644039154,
0.022612620145082474,
-0.06255343556404114,
-0.03646678850054741,
-0.05921369418501854,
-0.04698209837079048,
-0.04388175159692764,
-0.05506771430373192,
0.030511165037751198,
0.025596894323825836,
-0.03012026660144329,
-0.07994264364242554,
-0.022816065698862076,
0.013509931042790413,
0.0141273969784379,
-0.04801280423998833,
-0.015875602141022682,
0.00739283999428153,
0.012919665314257145,
-0.008783546276390553,
-0.055799730122089386,
0.011657399125397205,
-0.0001814405113691464,
-0.12641070783138275,
-0.029184190556406975,
-0.06092991307377815,
-0.017529692500829697,
0.05772669240832329,
0.009183174930512905,
0.026684775948524475,
-0.02781192772090435,
-0.028825098648667336,
0.023767292499542236,
0.029088512063026428,
0.003342385170981288,
0.054718017578125,
0.00491018733009696,
0.10132013261318207,
0.1314290463924408,
-0.05065545439720154,
0.07534534484148026,
0.047059573233127594,
-0.03154413774609566,
-0.028882555663585663,
0.00032438154448755085,
-0.02465124800801277,
0.022692792117595673,
-0.020514868199825287,
-0.09816638380289078,
0.01894908770918846,
0.0921359658241272,
-0.001075870357453823,
-0.03007924184203148,
0.056872159242630005,
-0.0813421756029129,
0.07721032947301865,
0.011569340713322163,
-0.015349753201007843,
0.038694098591804504,
-0.06196853518486023,
-0.06232496351003647,
0.004848065320402384,
0.0854317918419838,
-0.2503356635570526,
-0.0010915270540863276,
-0.022947821766138077,
0.006639119237661362,
-0.00953164603561163,
0.02875642478466034,
0.003725330112501979,
0.05183872580528259,
-0.07098809629678726,
0.01531099434942007,
0.02363181859254837,
0.007243557833135128,
0.06704931706190109,
-0.04099888727068901,
-0.018681522458791733,
0.01892494037747383,
0.06416428834199905,
-0.037744615226984024,
0.013658842071890831,
-0.04486986622214317,
0.013121779076755047,
0.02838120423257351,
0.12634825706481934,
-0.03578078746795654,
0.007891764864325523,
0.04742327705025673,
0.025328153744339943,
0.04246557131409645,
-0.07379947602748871,
0.0041883098892867565,
-0.02141856774687767,
-0.0018166181398555636,
0.06907203793525696,
-0.04896268993616104,
0.034405309706926346,
0.011917420662939548,
-0.0323496013879776,
-0.0035677053965628147,
0.02078963816165924,
-0.01168364193290472,
-0.05234409123659134,
0.014784686267375946,
-0.018277345225214958,
-0.03203659877181053,
0.04037738963961601,
-0.05152677744626999,
-0.019254080951213837,
-0.06626441329717636,
-0.03453097119927406,
0.02393239736557007,
0.009902754798531532,
0.014612918719649315,
-0.0032687124330550432,
0.00177537533454597,
0.018001791089773178,
0.014300416223704815,
-0.0003003064775839448,
-0.02530336007475853,
0.05651480704545975,
-0.05097286030650139,
-0.07676821947097778,
0.034244995564222336,
-0.08765088766813278,
0.09421998262405396,
-0.010337474755942822
] |
PMID:25037 | Relationship of pH of blood and aqueous with vitamin C. | Blood pH, aqueous pH, and intraocular tension were noted in 20 albino rabbits before and after administration of vitamin C in oral dosage of 7.5 gm/kg/day (in 4 equal divided doses for one week). A significant difference was noted in changes of blood pH and fall in intraocular tension but aqueous pH did not show any change. In 20 glaucoma patients there was no significant change in pH of blood of glaucoma after administration of vitamin C in 3.5 gm/kg body weight per day by oral route for one week (in 4 equal divided doses). | Relationship of pH of blood and aqueous with vitamin C. Blood pH, aqueous pH, and intraocular tension were noted in 20 albino rabbits before and after administration of vitamin C in oral dosage of 7.5 gm/kg/day (in 4 equal divided doses for one week). A significant difference was noted in changes of blood pH and fall in intraocular tension but aqueous pH did not show any change. In 20 glaucoma patients there was no significant change in pH of blood of glaucoma after administration of vitamin C in 3.5 gm/kg body weight per day by oral route for one week (in 4 equal divided doses). | [
-0.011653007939457893,
0.010211452841758728,
0.030936215072870255,
0.047476429492235184,
-0.012026982381939888,
-0.029222026467323303,
0.10866661369800568,
0.03310777619481087,
-0.04553437605500221,
0.026269275695085526,
0.02449127845466137,
-0.08465725183486938,
0.024891788139939308,
0.03696528449654579,
-0.04147019982337952,
-0.014197217300534248,
0.0009814552031457424,
-0.04748575761914253,
-0.10870710760354996,
0.05091081187129021,
-0.003513172036036849,
-0.0368613563477993,
0.020125145092606544,
-0.005025468301028013,
0.020313207060098648,
0.01243822742253542,
-0.025259125977754593,
-0.03956851735711098,
-0.019669540226459503,
-0.19349853694438934,
-0.008835280314087868,
0.0357089564204216,
0.009649521671235561,
-0.002349464688450098,
-0.024818459525704384,
0.03387844190001488,
0.017297953367233276,
0.01144876517355442,
0.029972493648529053,
0.07056144624948502,
0.062320247292518616,
0.0022467595990747213,
0.016637949272990227,
0.04087454080581665,
-0.04166453331708908,
0.038052741438150406,
-0.00991496630012989,
0.04780024662613869,
0.13104555010795593,
0.07144977152347565,
-0.019153650850057602,
0.00378344114869833,
-0.015666615217924118,
0.038652170449495316,
-0.0708831399679184,
-0.01061035506427288,
0.06264277547597885,
-0.025462374091148376,
0.0636194571852684,
0.038078032433986664,
0.001642773044295609,
-0.010611374862492085,
-0.15598289668560028,
0.09730921685695648,
0.03331471234560013,
0.0264729056507349,
-0.019648320972919464,
-0.0036557160783559084,
0.008028891868889332,
0.03517577424645424,
-0.07313548028469086,
0.04670887440443039,
-0.01801535114645958,
0.006881073582917452,
0.021105166524648666,
-0.04831208288669586,
-0.002376007614657283,
-0.0343744158744812,
0.02213415876030922,
-0.012655472382903099,
-0.033945631235837936,
-0.0014691143296658993,
-0.03545445203781128,
-0.027097666636109352,
0.052698131650686264,
-0.04198162257671356,
0.01183388102799654,
-0.03419739753007889,
-0.010540173389017582,
-0.03034883178770542,
-0.02257358655333519,
-0.046420060098171234,
-0.05430026724934578,
0.04585151746869087,
-0.013603734783828259,
0.013316739350557327,
-0.0023025372065603733,
-0.09168124943971634,
-0.019026368856430054,
0.3231807053089142,
-0.08240628987550735,
-0.0415949709713459,
-0.057426437735557556,
-0.054539356380701065,
0.014497706666588783,
-0.03180807828903198,
0.033778853714466095,
0.017322808504104614,
0.029627973213791847,
0.06431509554386139,
0.031941577792167664,
0.02230338752269745,
-0.003623104654252529,
-0.07349957525730133,
-0.07005345076322556,
0.06057494878768921,
0.05676628276705742,
-0.010691555216908455,
0.006748555693775415,
0.02115044556558132,
-0.04915492236614227,
-0.01974346674978733,
-0.014474963769316673,
-0.012488845735788345,
0.06034200266003609,
0.013512754812836647,
0.038002073764801025,
0.07566939294338226,
0.014899330213665962,
0.022781601175665855,
0.03515419736504555,
-0.006429605185985565,
0.010700881481170654,
-0.010720149613916874,
-0.016709772869944572,
0.009377011097967625,
0.02062631957232952,
-0.021056601777672768,
0.028851328417658806,
0.010963265784084797,
-0.08034449815750122,
-0.04621528834104538,
-0.039358727633953094,
-0.068296417593956,
-0.012349765747785568,
0.11230511963367462,
0.00835825689136982,
0.04080117121338844,
-0.05721447616815567,
-0.023719973862171173,
0.02544996701180935,
0.05317206680774689,
0.01560977939516306,
0.04817194491624832,
0.010145467706024647,
-0.0040948232635855675,
-0.006449057720601559,
-0.010642229579389095,
0.010987777262926102,
0.020563820376992226,
-0.05057496950030327,
-0.05542299896478653,
-0.03004802204668522,
-0.0006016475963406265,
0.029093757271766663,
-0.017162960022687912,
0.01623527519404888,
-0.04772438481450081,
0.030010893940925598,
-0.0014631790108978748,
0.05687972530722618,
0.01451075915247202,
-0.017815744504332542,
-0.0767427533864975,
0.02176440693438053,
0.03864337503910065,
-0.005577641073614359,
-0.005924406927078962,
0.07311750948429108,
-0.014964708127081394,
0.06541059166193008,
0.06372325867414474,
0.025874359533190727,
0.04429778456687927,
0.02123401314020157,
-0.001259012147784233,
-0.09065303951501846,
0.05353657528758049,
0.0021200994960963726,
0.03777606785297394,
-0.028886931017041206,
-0.029668858274817467,
-0.07232853025197983,
-0.00487842783331871,
-0.014418025501072407,
0.03384949266910553,
-0.06214160472154617,
-0.07833226025104523,
-0.05130098760128021,
0.0016146450070664287,
0.08174696564674377,
-0.06601420044898987,
-0.002613675780594349,
-0.006811406463384628,
0.057850174605846405,
0.010682501830160618,
0.012009277939796448,
0.010488053783774376,
0.02008773386478424,
-0.024776868522167206,
0.0014221908058971167,
0.007444582413882017,
-0.06464693695306778,
0.0036786231212317944,
-0.028247572481632233,
0.007448122370988131,
0.012602721340954304,
-0.01238301582634449,
0.001851447974331677,
0.007708478718996048,
0.04896882176399231,
-0.0973932221531868,
-0.016603831201791763,
-0.2910611629486084,
0.014556527137756348,
0.0159162525087595,
-0.020677315071225166,
0.10238611698150635,
-0.05198346823453903,
0.0004231808125041425,
0.01402347069233656,
-0.014117551036179066,
-0.007320272736251354,
0.035914402455091476,
0.051087841391563416,
-0.02569827251136303,
-0.05889222025871277,
0.0022391038946807384,
0.03012571670114994,
0.10954000055789948,
-0.011451173573732376,
0.10669131577014923,
-0.0018234930466860533,
0.035634275525808334,
-0.009126846678555012,
0.012791471555829048,
0.06804639846086502,
0.03936264291405678,
0.04604466259479523,
0.12626151740550995,
-0.0980115756392479,
-0.07215341180562973,
-0.015707027167081833,
0.0070092384703457355,
0.032574478536844254,
-0.030547618865966797,
-0.09166967868804932,
-0.026470765471458435,
0.008697793819010258,
0.0038314461708068848,
0.035617198795080185,
-0.05365009605884552,
0.014693430624902248,
0.014295918866991997,
0.0061773331835865974,
-0.037915050983428955,
-0.01870749704539776,
-0.041207000613212585,
0.07413064688444138,
0.012115802615880966,
0.0738491490483284,
-0.036413658410310745,
-0.009684242308139801,
0.03323456272482872,
-0.007641206961125135,
-0.05212702602148056,
-0.006217620335519314,
0.0083855502307415,
0.015314958989620209,
-0.059098247438669205,
0.033272452652454376,
-0.0904538705945015,
0.006223960313946009,
-0.006313876714557409,
0.0011849494185298681,
0.005505294073373079,
-0.026705987751483917,
0.008673823438584805,
-0.05246252194046974,
0.09730213135480881,
-0.01314008329063654,
0.026646340265870094,
0.08329271525144577,
0.014518526382744312,
0.09914729744195938,
-0.05379295349121094,
-0.014400621876120567,
0.03393339365720749,
0.03428826108574867,
-0.035359662026166916,
-0.03836826607584953,
-0.011955167166888714,
-0.00703910319134593,
0.025811389088630676,
-0.05973784253001213,
0.06588314473628998,
-0.006370798218995333,
0.034557681530714035,
-0.012531334534287453,
-0.016420962288975716,
-0.03455411642789841,
-0.051218073815107346,
0.025456948205828667,
0.04365710914134979,
-0.05337672680616379,
-0.03215332329273224,
-0.047193191945552826,
0.04738888144493103,
0.03915805369615555,
-0.2779306471347809,
-0.010475263930857182,
0.022906241938471794,
0.012050708755850792,
-0.031281378120183945,
0.027936555445194244,
0.016190150752663612,
0.0079096844419837,
-0.04534033685922623,
0.03424067795276642,
0.009089869447052479,
-0.0018341055838391185,
0.03620285168290138,
-0.006455646362155676,
0.023923398926854134,
-0.02362404577434063,
0.059903986752033234,
-0.024677932262420654,
0.01298270933330059,
0.024419400840997696,
0.0367283821105957,
-0.043133173137903214,
0.1221064105629921,
-0.031067930161952972,
-0.019813621416687965,
0.003517006989568472,
0.012626509182155132,
0.04516888037323952,
-0.014637798070907593,
0.016531262546777725,
0.01048241090029478,
0.0973409116268158,
0.01761705055832863,
-0.0193572249263525,
0.04156501591205597,
0.028409268707036972,
-0.0757344663143158,
0.00702610844746232,
0.006880124565213919,
0.014263653196394444,
-0.0066794659942388535,
0.017705250531435013,
-0.04910790175199509,
-0.010893619619309902,
0.05999353900551796,
-0.021194573491811752,
-0.04346928745508194,
-0.04001162201166153,
0.005950648337602615,
-0.059370145201683044,
-0.060526132583618164,
-0.07194066047668457,
0.09279128909111023,
0.006297807674854994,
-0.022115463390946388,
-0.011605823412537575,
-0.04281318932771683,
0.003913491033017635,
0.04994243010878563,
-0.04849398881196976,
0.02946295589208603,
-0.015538129024207592,
-0.03158239275217056,
-0.00012183230865048245,
0.014944511465728283
] |
PMID:25039 | Origin of an elevated plasma alkaline phosphatase activity in non-jaundiced patients. | Samples from 260 non-jaundiced patients with elevated plasma alkaline phosphatase activities were analysed for gamma-glutamyltransferase and 5'-nucleotidase activity, and for alkaline phosphatase isoenzyme pattern. The plasma gamma-glutamyltransferase activity was found to be a more sensitive index than that of plasma 5'-nucleotidase in confirming the presence of a liver component of the elevated plasma alkaline phosphatase. If the gamma-glutamyltransferase level is normal it is probable that the increase in plasma alkaline phosphatase activity is of bone origin. However, and elevated gamma-glutamyltransferase result does not exclude a bone component; in this situation plasma alkaline phosphatase isoenzymes should be estimated. The causes of elevated activities of plasma alkaline phosphatase, 5'-nucleotidase and gamma-glutamyltransferase, found in this investigation were generally the same as those found by other worker. The effect of treatment by drugs on gamma-glutamyltransferase, an inducible enzyme, needs more investigation. | Origin of an elevated plasma alkaline phosphatase activity in non-jaundiced patients. Samples from 260 non-jaundiced patients with elevated plasma alkaline phosphatase activities were analysed for gamma-glutamyltransferase and 5'-nucleotidase activity, and for alkaline phosphatase isoenzyme pattern. The plasma gamma-glutamyltransferase activity was found to be a more sensitive index than that of plasma 5'-nucleotidase in confirming the presence of a liver component of the elevated plasma alkaline phosphatase. If the gamma-glutamyltransferase level is normal it is probable that the increase in plasma alkaline phosphatase activity is of bone origin. However, and elevated gamma-glutamyltransferase result does not exclude a bone component; in this situation plasma alkaline phosphatase isoenzymes should be estimated. The causes of elevated activities of plasma alkaline phosphatase, 5'-nucleotidase and gamma-glutamyltransferase, found in this investigation were generally the same as those found by other worker. The effect of treatment by drugs on gamma-glutamyltransferase, an inducible enzyme, needs more investigation. | [
-0.018931305035948753,
0.024595580995082855,
-0.015859883278608322,
-0.0018761116079986095,
0.020630953833460808,
-0.06027017533779144,
0.06440696120262146,
0.0547650046646595,
-0.039058443158864975,
-0.059553880244493484,
0.032052695751190186,
-0.0546470507979393,
-0.0158136747777462,
0.00919982511550188,
-0.067356638610363,
-0.0769333764910698,
0.031067494302988052,
-0.03344724327325821,
-0.03337067365646362,
-0.010047564283013344,
-0.02146138809621334,
0.023971986025571823,
0.10578790307044983,
-0.01303690206259489,
0.055246319621801376,
0.06144765391945839,
0.006533874198794365,
0.012287057004868984,
-0.07165367901325226,
-0.17626546323299408,
-0.03742571175098419,
0.007769898045808077,
0.03092777170240879,
-0.03822101280093193,
-0.01443331316113472,
0.03917422518134117,
-0.03531290218234062,
0.057920463383197784,
-0.019499294459819794,
0.049996353685855865,
0.003662863513454795,
-0.012959197163581848,
-0.02873808518052101,
0.021902522072196007,
0.00791907962411642,
0.0020415717735886574,
-0.04660249873995781,
0.019064515829086304,
0.058968011289834976,
0.04504697397351265,
-0.028643352910876274,
0.028502989560365677,
0.010411050170660019,
0.05666668340563774,
-0.0016677394742146134,
0.009659242816269398,
0.03564979135990143,
0.0703161433339119,
-0.029296966269612312,
0.04630324989557266,
0.010457942262291908,
-0.021435290575027466,
-0.14186206459999084,
0.033328693360090256,
0.0940152108669281,
0.007865107618272305,
0.02728978730738163,
-0.06573425978422165,
0.03478514030575752,
0.03862755373120308,
-0.05341149866580963,
0.0598297081887722,
-0.044912561774253845,
0.04469699412584305,
0.03089538775384426,
-0.006470765918493271,
0.008236217312514782,
-0.0013443112839013338,
0.01308275293558836,
0.01937243714928627,
-0.030055036768317223,
0.0234798863530159,
0.0242434274405241,
0.01024444680660963,
-0.02890891209244728,
-0.03240878880023956,
0.015723031014204025,
-0.009330964647233486,
-0.035736314952373505,
0.038270916789770126,
-0.04305468499660492,
-0.054883625358343124,
-0.015224971808493137,
-0.0009692077874206007,
-0.048710934817790985,
0.040648456662893295,
0.06976167112588882,
0.048090752214193344,
0.014253225177526474,
0.3828641474246979,
-0.030625218525528908,
0.015268108807504177,
-0.10573320090770721,
0.05103203281760216,
0.00013484568626154214,
-0.028176162391901016,
0.010525304824113846,
-0.018463678658008575,
-0.030852967873215675,
-0.005061588250100613,
0.05685341730713844,
0.017701931297779083,
0.024589965119957924,
-0.07388344407081604,
-0.07352840900421143,
0.012035123072564602,
0.009619242511689663,
0.0067587741650640965,
-0.04797893390059471,
0.04835369065403938,
0.008974459022283554,
-0.030856529250741005,
-0.005866900086402893,
-0.08506162464618683,
0.005360478535294533,
0.08770478516817093,
0.004176913760602474,
0.07507825642824173,
0.06676188111305237,
-0.003142203902825713,
0.07133369892835617,
-0.01495268102735281,
-0.009099199436604977,
0.007369291502982378,
0.020916691049933434,
-0.02756400965154171,
0.05631685629487038,
-0.015513544902205467,
0.049028195440769196,
0.02236083894968033,
-0.009631823748350143,
-0.05412118881940842,
-0.0822119265794754,
-0.04124583303928375,
0.004307950846850872,
0.06721719354391098,
-0.03414887189865112,
0.044383808970451355,
0.025388281792402267,
-0.05449838936328888,
0.004135743249207735,
0.04513118788599968,
-0.034817811101675034,
0.05897461250424385,
-0.04520835727453232,
-0.04150361195206642,
0.009822537191212177,
-0.0056419349275529385,
-0.0224712323397398,
-0.04601099342107773,
-0.07345480471849442,
-0.02332560159265995,
0.007902223616838455,
0.042124368250370026,
0.056578151881694794,
-0.002240955363959074,
-0.031334325671195984,
-0.01525751780718565,
0.017151106148958206,
-0.036044590175151825,
-0.014605119824409485,
0.01818733848631382,
-0.02075224183499813,
-0.008206584490835667,
0.033038947731256485,
-0.0016964796232059598,
0.025817235931754112,
0.011730102822184563,
-0.0060927593149244785,
-0.04095747321844101,
0.04663531482219696,
0.0011319260811433196,
-0.018852561712265015,
0.05259723588824272,
-0.013663103803992271,
-0.05402928963303566,
-0.003932972904294729,
0.034252557903528214,
0.03813245892524719,
0.03107341006398201,
-0.019234489649534225,
-0.0037738822866231203,
-0.10475362092256546,
-0.031114602461457253,
-0.01085149496793747,
-0.06366384774446487,
-0.054750002920627594,
0.0011539945844560862,
0.045934587717056274,
-0.015607076697051525,
0.07331289350986481,
0.05873415991663933,
0.03162040188908577,
0.0004378875601105392,
-0.004419438075274229,
0.03314368799328804,
-0.06302332133054733,
0.025244884192943573,
0.07900945097208023,
0.09250171482563019,
0.014463716186583042,
0.031237291172146797,
-0.04108572378754616,
0.05033698305487633,
-0.041700128465890884,
0.012427770532667637,
-0.03839988633990288,
0.000893201504368335,
0.02862360328435898,
0.007231687195599079,
-0.006872444413602352,
-0.05651247128844261,
-0.03981926292181015,
-0.28225067257881165,
-0.014547672122716904,
0.03443676605820656,
0.013357851654291153,
-0.022404076531529427,
0.022588469088077545,
0.030344923958182335,
-0.02671695314347744,
-0.04899641498923302,
-0.006448616273701191,
0.07359454035758972,
0.07653916627168655,
-0.03379835933446884,
-0.033541105687618256,
0.01815141923725605,
0.03183360770344734,
-0.021768055856227875,
-0.0292807649821043,
0.04633820801973343,
-0.016976282000541687,
0.023668240755796432,
-0.05345144495368004,
0.04738922417163849,
0.026322724297642708,
0.04909508302807808,
-0.008871952071785927,
0.10175944119691849,
-0.007989209145307541,
-0.03509766608476639,
-0.058312974870204926,
0.009386271238327026,
0.0363960899412632,
-0.005927704740315676,
-0.10251864790916443,
-0.0010471550049260259,
-0.0592162199318409,
-0.054292693734169006,
-0.011444921605288982,
-0.038363952189683914,
0.030261550098657608,
-0.06512289494276047,
-0.034896332770586014,
-0.037841614335775375,
-0.03314363583922386,
-0.031185850501060486,
0.0008572239894419909,
0.006595755461603403,
0.00871484074741602,
0.05514483153820038,
-0.02364170551300049,
0.057484280318021774,
0.0051536764949560165,
0.02613404020667076,
-0.03634060546755791,
-0.005920134484767914,
-0.026010911911725998,
-0.06106899678707123,
0.02308128960430622,
-0.07030654698610306,
0.024486791342496872,
-0.027273353189229965,
0.0013817984145134687,
-0.010332350619137287,
-0.003010263666510582,
0.0005354617605917156,
-0.01729986071586609,
0.0024217315949499607,
0.024920031428337097,
0.07907508313655853,
0.030077097937464714,
0.000429094594437629,
0.107896588742733,
-0.03578321263194084,
-0.015771934762597084,
0.03611856698989868,
0.01153833419084549,
-0.046144574880599976,
-0.029963117092847824,
-0.02049853280186653,
0.0050246757455170155,
-0.008011418394744396,
-0.05110306292772293,
0.0867299884557724,
-0.011559825390577316,
-0.005671994294971228,
0.01815846562385559,
0.03691656142473221,
0.01964164897799492,
0.0023397200275212526,
0.017860298976302147,
0.034712281078100204,
-0.01046828180551529,
-0.05185279995203018,
-0.09037833660840988,
0.014922266826033592,
0.026926357299089432,
-0.2796842157840729,
0.013036934658885002,
-0.04504387453198433,
-0.08907143771648407,
0.0003656070039141923,
-0.004062778316438198,
0.023105716332793236,
0.03375442698597908,
-0.0208294615149498,
0.06804099678993225,
0.014458693563938141,
0.03810438513755798,
0.0651155635714531,
0.007472512312233448,
-0.06116648018360138,
-0.0022175658959895372,
0.04032985121011734,
0.03178757429122925,
0.039181482046842575,
-0.030639013275504112,
0.010878821834921837,
-0.056199923157691956,
0.1503361463546753,
0.007276087533682585,
0.029050221666693687,
0.06517365574836731,
-0.022338885813951492,
-0.015396383590996265,
-0.04674617201089859,
-0.0034156430047005415,
0.028729287907481194,
0.017956474795937538,
0.08642112463712692,
-0.003886937163770199,
-0.015612591989338398,
0.10100823640823364,
0.02048521302640438,
0.02746485359966755,
-0.050288453698158264,
-0.01905633509159088,
0.03551829978823662,
0.03304167836904526,
-0.07147949188947678,
-0.03719498589634895,
0.07100845128297806,
-0.09088634699583054,
0.00835033692419529,
-0.02659795992076397,
0.02450060099363327,
0.005594943184405565,
-0.0016868773382157087,
-0.022299202159047127,
-0.009950065985321999,
0.061971940100193024,
0.012923779897391796,
-0.016232891008257866,
-0.05857380852103233,
0.02294352650642395,
0.005365623626857996,
-0.05676981061697006,
0.005070780403912067,
0.01685968041419983,
-0.03302861005067825,
0.07518725842237473,
-0.02699192427098751
] |
PMID:25043 | Purification and some properties of riboflavin synthetase from Bacillus stearothermophilus ATCC 8005. | A riboflavin synthetase was purified 51-fold from a thermophilic organism, Bacillus stearothermophilus ATCC 8005, that grew at 40 to 72 degrees C. Some of the properties of the enzyme are: (i) its temperature optimum was 95 degrees C, and the activity was negligible below 40 degrees C; (ii) the Arrhenius plot of the initial reaction rates was concave upward, with a break at 65 degrees C, and the apparent activation energies below and above 65 degrees C were 4.2 X 10(4) and 6.7 X 10(4) J/mol, respectively; (iii) the enzyme was fairly stable up to 60 degrees C without 6,7-dimethyl-8-ribityllumazine; this substance protected the enzyme from inactivation above 60 to 97 degrees C; (iv) the pH range for stability was 6.0 to 10.0 at 26 degrees C and 6.3 to 7.6 at 55 degrees C; (v) the enzyme was highly resistant at 26 degrees C to denaturation in 8 M urea, but the tolerance was extremely low at 55 degrees C; (vi) its molecular weight was estimated at 45,000; (vii) the Km for 6,7-dimethyl-8-ribityllumazine was 23 micrometer at 55 degrees C and 29 micrometer at 75 degrees C; (viii) its pH optimum was 6.7 to 7.2; (ix) 6-methyl-7-hydroxy-8-ribityllumazine was a competitive inhibitor (Ki = 0.18 micrometer); (x) the activity was sensitive to heavy-metal ions and thiol reagents; (xi) the enzyme did not require cofactor or a carbon donor; and (xii) the molar ratio of 6,7-dimethyl-8-ribityllumazine consumption to riboflavin formation was 2 throughout the entire reaction. Properties i through vi distinguish this enzyme from riboflavin synthetases purified by other investigators from mesophilic organisms, Ashbya gossypii, Eremothecium ashbyii, Escherichia coli, yeast, and spinach. | Purification and some properties of riboflavin synthetase from Bacillus stearothermophilus ATCC 8005. A riboflavin synthetase was purified 51-fold from a thermophilic organism, Bacillus stearothermophilus ATCC 8005, that grew at 40 to 72 degrees C. Some of the properties of the enzyme are: (i) its temperature optimum was 95 degrees C, and the activity was negligible below 40 degrees C; (ii) the Arrhenius plot of the initial reaction rates was concave upward, with a break at 65 degrees C, and the apparent activation energies below and above 65 degrees C were 4.2 X 10(4) and 6.7 X 10(4) J/mol, respectively; (iii) the enzyme was fairly stable up to 60 degrees C without 6,7-dimethyl-8-ribityllumazine; this substance protected the enzyme from inactivation above 60 to 97 degrees C; (iv) the pH range for stability was 6.0 to 10.0 at 26 degrees C and 6.3 to 7.6 at 55 degrees C; (v) the enzyme was highly resistant at 26 degrees C to denaturation in 8 M urea, but the tolerance was extremely low at 55 degrees C; (vi) its molecular weight was estimated at 45,000; (vii) the Km for 6,7-dimethyl-8-ribityllumazine was 23 micrometer at 55 degrees C and 29 micrometer at 75 degrees C; (viii) its pH optimum was 6.7 to 7.2; (ix) 6-methyl-7-hydroxy-8-ribityllumazine was a competitive inhibitor (Ki = 0.18 micrometer); (x) the activity was sensitive to heavy-metal ions and thiol reagents; (xi) the enzyme did not require cofactor or a carbon donor; and (xii) the molar ratio of 6,7-dimethyl-8-ribityllumazine consumption to riboflavin formation was 2 throughout the entire reaction. Properties i through vi distinguish this enzyme from riboflavin synthetases purified by other investigators from mesophilic organisms, Ashbya gossypii, Eremothecium ashbyii, Escherichia coli, yeast, and spinach. | [
-0.10196886211633682,
-0.029832806438207626,
-0.06321381777524948,
0.028009111061692238,
0.028100159019231796,
-0.009514302015304565,
0.029848290607333183,
0.017721345648169518,
-0.07671529054641724,
-0.009102648124098778,
0.006792490836232901,
-0.09030960500240326,
0.034294769167900085,
-0.045866820961236954,
-0.0027852097991853952,
-0.03789972886443138,
0.05428614839911461,
0.032316066324710846,
-0.014927790500223637,
0.020348556339740753,
0.011061511002480984,
-0.020154530182480812,
0.06144123896956444,
0.0018871549982577562,
0.05065048485994339,
0.04837741330265999,
0.04324602335691452,
0.05200323089957237,
-0.020677201449871063,
-0.22364486753940582,
0.06052602082490921,
-0.0020314445719122887,
0.046720124781131744,
-0.06317569315433502,
-0.009141246788203716,
0.047116898000240326,
-0.02306375838816166,
-0.048790134489536285,
-0.033653661608695984,
0.06544218957424164,
0.010173969902098179,
0.03581547364592552,
-0.005987487733364105,
0.02105727046728134,
-0.031031938269734383,
0.08465787768363953,
-0.011036820709705353,
0.03540651500225067,
0.0931132584810257,
0.010394576005637646,
-0.03451265022158623,
-0.009480313397943974,
-0.00395511370152235,
0.03587275370955467,
0.04850039631128311,
-0.023380832746624947,
0.09078642725944519,
0.0722384825348854,
-0.004634385462850332,
-0.021118950098752975,
-0.002485445933416486,
-0.045089542865753174,
-0.08505138754844666,
0.04096848890185356,
0.026061242446303368,
0.006062139756977558,
0.028880229219794273,
-0.1077180802822113,
0.0035756819415837526,
0.051747675985097885,
-0.039774153381586075,
0.09083367884159088,
-0.044410642236471176,
0.008070755749940872,
0.05125509575009346,
0.018821926787495613,
-0.03710031136870384,
0.012645476497709751,
0.06942448765039444,
0.015332164242863655,
0.0032412789296358824,
-0.016961265355348587,
0.019595451653003693,
0.06338624656200409,
-0.01780972257256508,
-0.005616193171590567,
0.05324166640639305,
0.018502814695239067,
0.02990257926285267,
0.06950166076421738,
-0.021455977112054825,
-0.03291609510779381,
-0.007213056553155184,
-0.04877708479762077,
-0.09111498296260834,
0.04865647479891777,
0.037224870175123215,
0.030720476061105728,
0.05774663761258125,
0.3754976987838745,
-0.04410145804286003,
0.009087691083550453,
-0.038010209798812866,
-0.007468921598047018,
0.016391662880778313,
-0.016330325976014137,
-0.028583824634552002,
0.027705011889338493,
0.031040290370583534,
-0.009320026263594627,
0.044263485819101334,
-0.041885606944561005,
-0.02943161502480507,
-0.018138393759727478,
-0.015845663845539093,
0.01735938899219036,
0.05587185174226761,
-0.00875620637089014,
-0.04936209321022034,
0.029405876994132996,
-0.06361320614814758,
0.00043917514267377555,
0.058403193950653076,
-0.018645962700247765,
0.02570990100502968,
0.020730970427393913,
-0.026272227987647057,
0.08038370311260223,
0.05716153606772423,
0.027926748618483543,
0.0410148985683918,
0.02805360220372677,
0.01053930539637804,
-0.02484970912337303,
0.022763250395655632,
-0.048063211143016815,
0.019884197041392326,
-0.022672567516565323,
0.029376322403550148,
-0.06062139943242073,
-0.013285713270306587,
-0.05106523260474205,
-0.04433269426226616,
-0.04247959330677986,
-0.040117643773555756,
0.049571190029382706,
-0.02380530536174774,
0.038701131939888,
-0.0037160245701670647,
-0.03684472292661667,
0.006154847331345081,
0.03657906502485275,
0.008304825983941555,
-0.014226111583411694,
0.029630297794938087,
-0.010542364791035652,
-0.05016258731484413,
-0.030410461127758026,
-0.006408356130123138,
-0.015764158219099045,
-0.09915518760681152,
-0.02411002106964588,
0.013407053425908089,
0.07217184454202652,
-0.05075275897979736,
-0.027770427986979485,
-0.04340313747525215,
0.02673899568617344,
-0.00689292885363102,
-0.026645442470908165,
0.032821692526340485,
-0.012719412334263325,
-0.05159316956996918,
-0.015965189784765244,
-0.039545610547065735,
-0.008200515061616898,
-0.0027987794019281864,
-0.018187614157795906,
-0.01329327467828989,
0.0007519429200328887,
0.004607856273651123,
0.01753496751189232,
-0.015224159695208073,
0.028204631060361862,
0.002714988309890032,
-0.028008341789245605,
-0.0666792169213295,
-0.02614426240324974,
-0.02015507034957409,
0.05613798275589943,
-0.049248162657022476,
-0.00632608262822032,
-0.07197006791830063,
0.05387142673134804,
-0.003913698252290487,
-0.010745315812528133,
-0.03788366541266441,
0.08682402223348618,
0.054808732122182846,
-0.02924632839858532,
0.07352698594331741,
0.010772638954222202,
-0.028591640293598175,
0.04313657060265541,
-0.01610729843378067,
0.02099781483411789,
0.04794161394238472,
0.021947670727968216,
-0.005044435150921345,
-0.0010598684893921018,
-0.0350043922662735,
0.032615795731544495,
-0.11653462052345276,
-0.005810827948153019,
-0.08414217084646225,
0.03825874999165535,
-0.03242574632167816,
-0.012257055379450321,
0.04255402833223343,
0.013822301290929317,
0.02370031177997589,
-0.07306786626577377,
-0.06427492946386337,
-0.2997434139251709,
0.02648570016026497,
0.020236073061823845,
-0.01326859649270773,
0.06708210706710815,
-0.02375832386314869,
0.01592988334596157,
-0.04553557187318802,
0.010881512425839901,
-0.052066728472709656,
0.03579280897974968,
0.06568359583616257,
-0.005579471588134766,
-0.06136858090758324,
-0.018164170905947685,
-0.011468935757875443,
0.051844239234924316,
-0.012570320628583431,
-0.0008647291106171906,
-0.04177054017782211,
0.037991322576999664,
0.025259679183363914,
-0.010350060649216175,
0.03664736822247505,
0.031437329947948456,
-0.04425342008471489,
0.07315855473279953,
-0.02885725535452366,
0.035660531371831894,
0.03535190597176552,
0.03924529254436493,
0.02933179959654808,
-0.032541368156671524,
-0.10604267567396164,
-0.020519644021987915,
-0.016377007588744164,
0.0011082924902439117,
-0.02366795763373375,
-0.013799226842820644,
-0.012152974493801594,
0.04108385369181633,
0.04725990071892738,
-0.019350670278072357,
-0.03422549366950989,
0.007326883263885975,
-0.03325266391038895,
0.03800993040204048,
0.020410733297467232,
-0.011730761267244816,
0.01679046079516411,
-0.007766449823975563,
0.0447503887116909,
0.04010779410600662,
0.04135116562247276,
-0.05080877244472504,
-0.018032843247056007,
-0.05372649431228638,
-0.0016518925549462438,
-0.02807324379682541,
0.01598404161632061,
-0.013302681036293507,
-0.04070809483528137,
-0.01631549373269081,
0.05602462589740753,
-0.008576955646276474,
0.0223519466817379,
0.044160254299640656,
0.0028451241087168455,
0.051811762154102325,
-0.014578494243323803,
-0.013949870131909847,
0.03662225231528282,
-0.024783851578831673,
-0.01310738269239664,
0.03085816651582718,
-0.008254819549620152,
-0.019039813429117203,
-0.02148175984621048,
-0.012147080153226852,
-0.0266164131462574,
0.01366275642067194,
0.04267555847764015,
0.03901030123233795,
-0.08202642947435379,
0.02800040878355503,
0.03885597363114357,
0.010159116238355637,
-0.03276212513446808,
0.07240293174982071,
0.0608338825404644,
0.06328888982534409,
-0.053421925753355026,
0.010434715077280998,
-0.024123139679431915,
0.0803479254245758,
0.0760577842593193,
-0.2606832981109619,
-0.01071687787771225,
0.02990737184882164,
-0.008911563083529472,
-0.06178297847509384,
0.019392142072319984,
0.034022849053144455,
-0.028852812945842743,
-0.031721554696559906,
-0.012858070433139801,
0.041247956454753876,
-0.005291256587952375,
0.05426569655537605,
0.03770894557237625,
0.029538985341787338,
-0.0238084327429533,
0.003387340111657977,
-0.03807491064071655,
0.03477916494011879,
-0.030273910611867905,
0.014391141943633556,
-0.029757671058177948,
0.12188787758350372,
-0.015500412322580814,
0.005611255299299955,
0.07586686313152313,
-0.008827575482428074,
0.046373914927244186,
-0.06472203880548477,
0.0285220667719841,
-0.008781643584370613,
0.04341459646821022,
0.10216914117336273,
-0.02225651778280735,
0.01797887124121189,
0.027627015486359596,
-0.018931347876787186,
-0.03302302211523056,
-0.02983337640762329,
-0.01098612230271101,
0.006024460308253765,
-0.015368240885436535,
-0.08026912063360214,
-0.023847727105021477,
0.08463946729898453,
-0.0933094173669815,
-0.0631461963057518,
-0.07531131058931351,
-0.06246058642864227,
-0.0014618376735597849,
-0.011066439561545849,
-0.00024118612054735422,
0.025494733825325966,
-0.0016000907635316253,
0.003563129110261798,
0.003165025729686022,
-0.04064294323325157,
-0.0013676946982741356,
-0.013326508924365044,
-0.04968361184000969,
-0.017621705308556557,
-0.006906046997755766,
-0.006161811295896769,
0.15529394149780273,
-0.03622553497552872
] |
PMID:25040 | A supplement to alkaline phosphatase fractionations: utilization of gamma-glutamyl transpeptidase and hydroxyproline assays. | Fractionation of serum alkaline phosphatase by either biochemical or electrophoretic techniques is often insufficient to determine the source of the elevated serum enzyme. In 31 unselected patients with high serum alkaline phosphatase a simultaneous determination of serum gamma glutamyl transpeptidase and urinary hydroxyproline excretion rates was performed, in addition to urea denaturation and L-phenylalanine inhibition tests. Of 25 patients with definite diagnosis, the urea denaturation test correctly identified the source of the elevated serum alkaline phosphatase (ALP) in 16 (64 percent). The serum gamma-glutamyl transpeptidase (GGTP) alone predicted the correct diagnosis in 64 percent of the cases and the urinary hydroxyproline (HOP) alone in 69 percent. When all three tests were performed simultaneously, the combination of results identified the source of the ALP in 88 percent of the cases. It is believed that this combination offers better sensitivity and specificity than any of the three tests used individually. | A supplement to alkaline phosphatase fractionations: utilization of gamma-glutamyl transpeptidase and hydroxyproline assays. Fractionation of serum alkaline phosphatase by either biochemical or electrophoretic techniques is often insufficient to determine the source of the elevated serum enzyme. In 31 unselected patients with high serum alkaline phosphatase a simultaneous determination of serum gamma glutamyl transpeptidase and urinary hydroxyproline excretion rates was performed, in addition to urea denaturation and L-phenylalanine inhibition tests. Of 25 patients with definite diagnosis, the urea denaturation test correctly identified the source of the elevated serum alkaline phosphatase (ALP) in 16 (64 percent). The serum gamma-glutamyl transpeptidase (GGTP) alone predicted the correct diagnosis in 64 percent of the cases and the urinary hydroxyproline (HOP) alone in 69 percent. When all three tests were performed simultaneously, the combination of results identified the source of the ALP in 88 percent of the cases. It is believed that this combination offers better sensitivity and specificity than any of the three tests used individually. | [
-0.032338641583919525,
0.01224400196224451,
-0.02172478474676609,
0.0008963920408859849,
0.002083668252453208,
-0.03782181069254875,
0.025572394952178,
0.0475909560918808,
-0.026133570820093155,
-0.018422972410917282,
0.015615047886967659,
-0.06508797407150269,
-0.010764663107693195,
0.033750589936971664,
-0.046582046896219254,
-0.04515359178185463,
0.06102133169770241,
-0.025423094630241394,
-0.034131139516830444,
0.015157345682382584,
0.008607929572463036,
0.05223928391933441,
0.04677320644259453,
-0.04831886664032936,
0.044247232377529144,
0.031199097633361816,
0.004949316848069429,
-0.002075866097584367,
-0.045033495873212814,
-0.1804029494524002,
-0.009084310382604599,
-0.029294155538082123,
-0.025122027844190598,
-0.03877587616443634,
-0.05244670435786247,
0.06100946292281151,
-0.02400171011686325,
0.0006961246253922582,
-0.0026886742562055588,
0.016646072268486023,
0.023009488359093666,
0.00686838710680604,
-0.030757222324609756,
-0.011492711491882801,
-0.004078484605997801,
0.03278883919119835,
-0.06746097654104233,
0.05191158503293991,
0.08156987279653549,
0.044079381972551346,
-0.007226466666907072,
0.05993043631315231,
0.07616259902715683,
0.02108338102698326,
-0.012192785739898682,
-0.02082802727818489,
0.010926834307610989,
0.05551254749298096,
-0.027304908260703087,
0.019591866061091423,
-0.01862192153930664,
0.006865984294563532,
-0.1560097485780716,
0.029443804174661636,
0.03003646992146969,
-0.00815852265805006,
-0.016569441184401512,
-0.05644906684756279,
0.029097573831677437,
0.03095865808427334,
-0.020182082429528236,
0.016188664361834526,
-0.009063857607543468,
0.03848165646195412,
-0.000988106825388968,
0.021721066907048225,
0.002000634092837572,
0.020439639687538147,
0.02076134830713272,
0.02986719459295273,
-0.024489929899573326,
0.03976645693182945,
0.020099589601159096,
0.03515452891588211,
0.005134097300469875,
-0.06050233170390129,
0.04446740821003914,
0.03401792421936989,
0.03240581974387169,
-0.006691351532936096,
-0.01628977432847023,
-0.06640274077653885,
-0.008959679864346981,
0.01773041859269142,
-0.044040221720933914,
0.02117026410996914,
0.02610599622130394,
0.031109359115362167,
0.0007170456810854375,
0.36777931451797485,
-0.047264061868190765,
0.005436899606138468,
-0.030837280675768852,
-0.0023651951923966408,
0.002747121499851346,
-0.03423400968313217,
-0.02668333798646927,
-0.05240461602807045,
-0.04426223039627075,
0.054616816341876984,
0.017671095207333565,
0.029856808483600616,
0.04987487196922302,
-0.0491567887365818,
-0.07141473889350891,
0.037496909499168396,
0.04253362491726875,
-0.002298017032444477,
-0.04197430983185768,
0.027567783370614052,
0.004749208688735962,
-0.0026919865049421787,
-0.009387359023094177,
-0.0451945886015892,
0.011834535747766495,
0.027199095115065575,
0.016274474561214447,
0.09781795740127563,
0.042731668800115585,
0.015622124075889587,
0.004616298247128725,
-0.0019687782041728497,
-0.024738729000091553,
0.0154340835288167,
-0.046643175184726715,
-0.024380702525377274,
0.07523984462022781,
-0.04359803348779678,
0.01875658892095089,
0.04441235587000847,
-0.025471601635217667,
-0.04370315372943878,
-0.08357462286949158,
-0.01657375879585743,
-0.022613391280174255,
0.15732434391975403,
-0.06671959906816483,
0.05136944726109505,
0.03374170884490013,
-0.10279988497495651,
-0.010182236321270466,
0.06155410036444664,
0.0001710880605969578,
0.05607956647872925,
-0.020482610911130905,
0.016415473073720932,
0.043517451733350754,
0.04713411629199982,
0.017059745267033577,
0.014455966651439667,
-0.08665867149829865,
-0.03921506553888321,
0.005095645785331726,
0.026013508439064026,
0.01779637485742569,
-0.0025367937050759792,
-0.060767028480768204,
-0.026210198178887367,
-0.0032411934807896614,
-0.04532889276742935,
0.04660722613334656,
0.025400623679161072,
0.00897938571870327,
-0.017031125724315643,
-0.027285870164632797,
-0.017803777009248734,
-0.009870581328868866,
0.04268321394920349,
0.02910497970879078,
-0.020479977130889893,
0.08148440718650818,
-0.008553768508136272,
-0.07931945472955704,
0.05871772766113281,
0.012722397223114967,
-0.048003800213336945,
0.001969078555703163,
0.01665101945400238,
0.019034182652831078,
0.03582454472780228,
-0.028587963432073593,
0.00854706671088934,
-0.08399373292922974,
-0.0301823690533638,
0.023249810561537743,
-0.023377656936645508,
-0.09470441937446594,
0.021889472380280495,
0.07198608666658401,
-0.003911319654434919,
0.09041529148817062,
0.005624053999781609,
0.010011319071054459,
0.01452452689409256,
-0.051610808819532394,
0.04872054606676102,
-0.03983500227332115,
0.04359104111790657,
0.05739463493227959,
0.06579657644033432,
-0.020008372142910957,
0.009770209901034832,
-0.03879832103848457,
0.02902350202202797,
-0.05458127334713936,
0.0384669192135334,
0.013004965148866177,
-0.009395739994943142,
-0.013445703312754631,
0.018549731001257896,
0.025779668241739273,
-0.04788757115602493,
-0.04264253377914429,
-0.30386894941329956,
-0.026773598045110703,
0.036262497305870056,
-0.0210402924567461,
0.04483993723988533,
-0.06757847964763641,
-0.01331390906125307,
-0.007309065666049719,
-0.06026717647910118,
-0.003779458813369274,
0.09570837765932083,
0.09502915292978287,
-0.033704280853271484,
0.014354473911225796,
0.019972149282693863,
0.041376661509275436,
-0.015580307692289352,
-0.014365563169121742,
0.02294839173555374,
0.007152230478823185,
0.021411724388599396,
-0.03677073493599892,
0.05491859093308449,
0.03199690580368042,
0.03969272971153259,
-0.006941758096218109,
0.10317708551883698,
-0.011650608852505684,
-0.042268481105566025,
-0.06029259040951729,
-0.02243957482278347,
0.003133852034807205,
-0.029715048149228096,
-0.08322837948799133,
0.01737137697637081,
-0.04246540740132332,
-0.07537087053060532,
-0.03698187693953514,
-0.014621361158788204,
-0.005144480615854263,
-0.025735588744282722,
-0.007288750726729631,
-0.03469446301460266,
-0.08936019986867905,
-0.04416172578930855,
-0.025898411870002747,
-0.0019835098646581173,
0.03199642151594162,
0.042153771966695786,
0.019959254190325737,
0.07475635409355164,
-0.004540933296084404,
0.033983636647462845,
-0.037235915660858154,
0.008848643861711025,
-0.011098789051175117,
-0.06392952799797058,
0.03202395886182785,
-0.022655166685581207,
-0.011254933662712574,
0.012896712869405746,
-0.01729588210582733,
0.00978537555783987,
0.031152494251728058,
0.025522423908114433,
-0.050104353576898575,
-0.018653670325875282,
0.006948693655431271,
0.08986236900091171,
0.07059767097234726,
0.03578146919608116,
0.06682024896144867,
-0.021593954414129257,
0.02628888376057148,
0.014492184855043888,
0.00365818222053349,
0.0032853316515684128,
-0.07275538891553879,
-0.03795671463012695,
-0.010300630703568459,
-0.013988500460982323,
-0.0375792495906353,
0.018170807510614395,
0.029940864071249962,
-0.021435242146253586,
0.06825699657201767,
0.05484108626842499,
0.00008209097723010927,
0.04064232110977173,
0.04170840233564377,
0.020713603124022484,
0.0013243794674053788,
-0.01911156252026558,
-0.056367192417383194,
-0.020938701927661896,
0.04134703055024147,
-0.3020780682563782,
0.009808567352592945,
-0.012106901034712791,
-0.05697118490934372,
-0.019889485090970993,
0.012946061789989471,
0.03855540230870247,
-0.010552135296165943,
-0.01758788712322712,
0.038274675607681274,
0.024580761790275574,
0.06363478302955627,
0.05157624930143356,
-0.013851186260581017,
-0.007198691833764315,
-0.008600990287959576,
0.0320475697517395,
0.0017554325750097632,
0.0368223711848259,
-0.032895322889089584,
0.02823854237794876,
-0.06379534304141998,
0.16996997594833374,
0.02962440811097622,
0.019996371120214462,
0.07073521614074707,
0.013919677585363388,
-0.028044922277331352,
-0.0022903825156390667,
-0.02133520506322384,
-0.0021568441297858953,
0.03802002966403961,
0.07657808810472488,
-0.042421065270900726,
0.0018019424751400948,
0.07058734446763992,
0.024262934923171997,
0.006097686477005482,
-0.03129275515675545,
-0.04204548895359039,
0.026547959074378014,
-0.0063643683679401875,
-0.0709744393825531,
-0.023151015862822533,
0.04410644993185997,
-0.1199508085846901,
-0.045196834951639175,
-0.03408493101596832,
-0.024240899831056595,
-0.003912404645234346,
-0.0072349729016423225,
-0.016470929607748985,
0.029792504385113716,
0.021635159850120544,
-0.01419831719249487,
-0.03047705627977848,
-0.022421900182962418,
-0.0019430130487307906,
-0.051895622164011,
-0.03474331647157669,
0.001897953450679779,
0.0166783407330513,
-0.03473774343729019,
0.0957375168800354,
0.004600021056830883
] |
PMID:25044 | Formation of glycosidases in batch and continuous culture of Bacteroides fragilis. | Nine strains of bacteroides fragilis were cultivated in stirred fermentors and tested for their ability to produce glycosidases. B. fragilis subsp. vulgatus B70 was used for optimizing the production of glycosidases. The highest bacterial yield was obtained in proteose peptone-yeast extract medium. The optimum pH for maximal bacterial yield was 7.0, and the optimum temperature for growth was 37 degrees C. The formation of glycosidases was optimal between pH 6.5 and 7.5, and the optimum temperature for synthesis of glycosidases was between 33 and 37 degrees C. Culture under controlled conditions in fermentors gave more reproducible production of glycosidases than static cultures in bottles. The strain was also grown in continuous culture at a dilution rate of 0.1 liter/h at pH 7.0 and 37 degrees C with a yield of 2.0 mg of dry weight per ml in the complex medium. The formation of glycosidases remained constant during the entire continuous process. | Formation of glycosidases in batch and continuous culture of Bacteroides fragilis. Nine strains of bacteroides fragilis were cultivated in stirred fermentors and tested for their ability to produce glycosidases. B. fragilis subsp. vulgatus B70 was used for optimizing the production of glycosidases. The highest bacterial yield was obtained in proteose peptone-yeast extract medium. The optimum pH for maximal bacterial yield was 7.0, and the optimum temperature for growth was 37 degrees C. The formation of glycosidases was optimal between pH 6.5 and 7.5, and the optimum temperature for synthesis of glycosidases was between 33 and 37 degrees C. Culture under controlled conditions in fermentors gave more reproducible production of glycosidases than static cultures in bottles. The strain was also grown in continuous culture at a dilution rate of 0.1 liter/h at pH 7.0 and 37 degrees C with a yield of 2.0 mg of dry weight per ml in the complex medium. The formation of glycosidases remained constant during the entire continuous process. | [
-0.014213426038622856,
-0.011990874074399471,
-0.04880470782518387,
-0.00644413847476244,
0.04859808459877968,
-0.05482695624232292,
-0.09421548992395401,
0.04808363318443298,
-0.04326355457305908,
-0.04379374533891678,
0.01617581769824028,
-0.06580445915460587,
-0.00041019104537554085,
-0.008211903274059296,
-0.024813508614897728,
-0.029356472194194794,
0.02808821015059948,
-0.018924297764897346,
-0.06889723986387253,
0.03973590210080147,
0.06013370305299759,
-0.022279152646660805,
0.01421068049967289,
-0.056215811520814896,
-0.014811525121331215,
-0.013421215116977692,
0.020444441586732864,
0.006345176137983799,
-0.0073227486573159695,
-0.17721709609031677,
0.02897784858942032,
-0.02789164148271084,
0.08029236644506454,
-0.023481937125325203,
-0.06822880357503891,
0.030210210010409355,
0.0089617520570755,
-0.03921423479914665,
0.012156822718679905,
0.0687694326043129,
0.04782874137163162,
0.03615133464336395,
-0.030928634107112885,
0.0025840331800282,
-0.018139632418751717,
-0.009022398851811886,
-0.027298247441649437,
0.020789917558431625,
0.08263362944126129,
0.016314642503857613,
-0.01979750581085682,
-0.03475835546851158,
-0.034465182572603226,
-0.02602194994688034,
-0.019603876397013664,
-0.01280179526656866,
-0.0019588693976402283,
0.015312202274799347,
-0.01303678285330534,
0.01603999175131321,
0.03256836533546448,
-0.031327374279499054,
-0.13406036794185638,
0.03628532215952873,
-0.017275432124733925,
-0.03171030059456825,
0.0015024481108412147,
-0.06728316843509674,
0.03950444236397743,
0.0723847970366478,
-0.03329591825604439,
0.03201406076550484,
-0.0056074014864861965,
0.009537607431411743,
0.020678235217928886,
0.015521949157118797,
-0.00458442373201251,
0.018617095425724983,
0.03449823707342148,
0.10493472218513489,
0.0016042609931901097,
0.022698651999235153,
-0.027188079431653023,
-0.0010437715100124478,
-0.03500194475054741,
-0.034319233149290085,
0.028735605999827385,
0.024019209668040276,
0.056302353739738464,
0.025473445653915405,
0.0024789334274828434,
-0.052459098398685455,
-0.06712880730628967,
-0.002959595061838627,
-0.021462760865688324,
0.02735273912549019,
0.009967592544853687,
0.046191271394491196,
-0.016042590141296387,
0.4095824360847473,
-0.07169254869222641,
-0.05824018269777298,
-0.0019070409471169114,
0.03430430591106415,
0.026746125891804695,
-0.04347415640950203,
-0.0342448465526104,
-0.027083449065685272,
-0.01422718446701765,
0.03014935366809368,
0.04581297188997269,
0.025136413052678108,
-0.003294941270723939,
-0.0554366260766983,
-0.012280954048037529,
0.0022922346834093332,
0.034720513969659805,
0.014935242012143135,
-0.0833369642496109,
0.05476691946387291,
0.002175849862396717,
-0.026390790939331055,
0.06247566267848015,
-0.02630935050547123,
0.057593341916799545,
0.02216436341404915,
-0.018667839467525482,
0.09308050572872162,
0.00807270873337984,
-0.03904928267002106,
0.023370590060949326,
0.030971765518188477,
-0.015972301363945007,
-0.01624041236937046,
0.02573392726480961,
-0.06603463739156723,
0.04198012501001358,
-0.017694033682346344,
0.03190638870000839,
-0.009751280769705772,
-0.009041819721460342,
-0.005106222350150347,
-0.051036518067121506,
-0.058492131531238556,
-0.01221877709031105,
0.07643824070692062,
-0.0346984937787056,
0.020115992054343224,
-0.023448562249541283,
-0.062181632965803146,
0.004042689688503742,
0.02365976758301258,
0.004553478676825762,
0.004783105105161667,
0.00941917859017849,
-0.006853334605693817,
0.003786388086155057,
-0.024265466257929802,
0.03356717526912689,
-0.03771962225437164,
-0.07119037210941315,
0.02867615967988968,
0.041894540190696716,
0.06038949266076088,
0.03769020736217499,
-0.01921801082789898,
-0.027842804789543152,
0.03675701096653938,
0.020831743255257607,
0.00046645975089631975,
0.02373775839805603,
-0.024663042277097702,
0.019981401041150093,
-0.01600046269595623,
-0.004689508117735386,
-0.004817686975002289,
0.008237974718213081,
0.029125012457370758,
0.015508079901337624,
0.04517329856753349,
0.05364101007580757,
-0.01161276362836361,
-0.01531956810504198,
0.022748518735170364,
0.024796586483716965,
-0.058459922671318054,
0.03168785944581032,
-0.011195652186870575,
0.0003105869109276682,
0.008584791794419289,
-0.03684886917471886,
-0.004511634819209576,
-0.12960124015808105,
0.01665889471769333,
0.06940724700689316,
-0.053099825978279114,
0.019075961783528328,
-0.0006305592833086848,
0.02478780597448349,
0.00046663969988003373,
0.03565916419029236,
0.023735638707876205,
0.002152038970962167,
-0.035008128732442856,
0.046038080006837845,
0.013966601341962814,
0.09611143171787262,
0.004254723899066448,
-0.02337803691625595,
0.001234009861946106,
-0.06772416085004807,
-0.024436214938759804,
0.007592780515551567,
0.006598362233489752,
-0.09905727952718735,
0.05590442568063736,
-0.031592242419719696,
-0.007175112143158913,
0.04153510928153992,
0.015160157345235348,
0.060568176209926605,
-0.020533595234155655,
-0.013157054781913757,
-0.28842833638191223,
0.018721887841820717,
0.04549530893564224,
-0.013017860241234303,
0.05022121220827103,
-0.05090666189789772,
0.05117898806929588,
-0.056081417948007584,
0.008583158254623413,
-0.04502196982502937,
0.0738547146320343,
0.03127281740307808,
-0.033159852027893066,
-0.04463668912649155,
0.0037941320333629847,
0.046705976128578186,
0.005989389959722757,
-0.014290938153862953,
0.03880707919597626,
-0.009380904026329517,
0.015506580471992493,
-0.0683174803853035,
0.08358044177293777,
-0.011478235945105553,
0.011637557297945023,
-0.043758492916822433,
0.10728628933429718,
-0.04501922056078911,
0.02732820250093937,
-0.07593461126089096,
0.06685731559991837,
-0.01627742499113083,
-0.013022823259234428,
-0.07167994976043701,
-0.01138195488601923,
-0.018642446026206017,
0.03503442928195,
-0.054712727665901184,
0.03382504731416702,
0.0006608408875763416,
0.04800770431756973,
0.0049923015758395195,
-0.056457407772541046,
-0.07718617469072342,
0.009637903422117233,
-0.08388675004243851,
0.04944482818245888,
-0.009091176092624664,
0.02949456498026848,
-0.01788630522787571,
0.01794174499809742,
-0.029069898650050163,
0.020566662773489952,
0.03322749212384224,
-0.04102321341633797,
0.0011118357069790363,
-0.020159246399998665,
0.03768179938197136,
-0.03997422754764557,
0.021663419902324677,
0.002783203264698386,
-0.03266557306051254,
0.030046496540308,
0.04642544686794281,
0.015085370279848576,
0.010386303067207336,
0.034732885658741,
-0.03795715421438217,
0.045329563319683075,
0.003473124699667096,
0.050763968378305435,
0.03725449740886688,
0.0008954485529102385,
0.016134876757860184,
0.037969790399074554,
0.036095209419727325,
-0.0049819909036159515,
-0.05070085823535919,
-0.11128278076648712,
0.006895550061017275,
-0.034510139375925064,
-0.01479642279446125,
0.05810989812016487,
0.02702457830309868,
0.04906380921602249,
0.06704587489366531,
-0.0015401332639157772,
-0.0012549537932500243,
0.002638779580593109,
0.06646871566772461,
0.04301400110125542,
-0.0021737648639827967,
-0.06923314183950424,
-0.022920958697795868,
0.035866498947143555,
0.029899951070547104,
-0.2545444667339325,
-0.0003044626791961491,
0.02700563333928585,
0.008171634748578072,
0.028659828007221222,
0.0196198970079422,
0.048599760979413986,
-0.02328583225607872,
0.021196622401475906,
0.07460945844650269,
0.06025322526693344,
-0.022193599492311478,
0.045359306037425995,
0.023312194272875786,
0.04721046984195709,
0.03012840822339058,
-0.0015735059278085828,
-0.01865302585065365,
0.08891081064939499,
-0.04622838273644447,
0.01977095939218998,
-0.05675717070698738,
0.11257001757621765,
0.05594586953520775,
-0.03511820733547211,
0.07857383787631989,
0.00018871577049139887,
0.036369774490594864,
-0.003777401754632592,
0.007842222228646278,
0.02592027746140957,
0.033874690532684326,
0.09064871817827225,
-0.05822505056858063,
-0.011687304824590683,
0.03447355702519417,
-0.002328382106497884,
0.006480028852820396,
-0.02603224664926529,
0.049703553318977356,
0.009263097308576107,
-0.040920112282037735,
-0.06316987425088882,
-0.030348876491189003,
0.030918247997760773,
-0.07670183479785919,
-0.04636348411440849,
-0.12533102929592133,
-0.028304725885391235,
-0.016144106164574623,
-0.057828858494758606,
-0.07154611498117447,
0.0016640258254483342,
-0.02199099212884903,
0.005120471585541964,
-0.007904049940407276,
-0.01672207936644554,
0.04351361468434334,
-0.03502688929438591,
0.006703756749629974,
0.028470750898122787,
-0.046760283410549164,
-0.02324678562581539,
0.11546341329813004,
-0.10705844312906265
] |
PMID:25045 | Clear, defined medium for the sporulation of Clostridium perfringens. | A new, defined medium for the sporulation of Clostridium perfringens is presented. Sporulation levels exceeding 10(6) to 10(7) heat-resistant spores per ml were obtained for seven strains: PS49, PS52, FD-1, T-65, NCTC strains 8798, 8238, and 10240. In the presence of theophylline, a methylxanthine, higher levels of heat-resistant spores were attained for strains PS49, PS52, FD-1, ant T-65; photomicrographs demonstrated a higher fraction of sporulating cells when these strains were grown in the presence of methylxanthines. Use of washed, highly diluted (less than 100 cells) inocula resulted in no reduction in spore yield. Strain KA3 grew well but sporulated poorly on this medium. The medium was clear and free of precipitate when small amounts (100 microgram/ml) of methylxanthine were incorporated. | Clear, defined medium for the sporulation of Clostridium perfringens. A new, defined medium for the sporulation of Clostridium perfringens is presented. Sporulation levels exceeding 10(6) to 10(7) heat-resistant spores per ml were obtained for seven strains: PS49, PS52, FD-1, T-65, NCTC strains 8798, 8238, and 10240. In the presence of theophylline, a methylxanthine, higher levels of heat-resistant spores were attained for strains PS49, PS52, FD-1, ant T-65; photomicrographs demonstrated a higher fraction of sporulating cells when these strains were grown in the presence of methylxanthines. Use of washed, highly diluted (less than 100 cells) inocula resulted in no reduction in spore yield. Strain KA3 grew well but sporulated poorly on this medium. The medium was clear and free of precipitate when small amounts (100 microgram/ml) of methylxanthine were incorporated. | [
-0.012135166674852371,
-0.010912828147411346,
-0.010668435133993626,
-0.002959358738735318,
0.07182912528514862,
-0.06377027928829193,
-0.05426190048456192,
0.0367794968187809,
-0.05047863721847534,
-0.010452923364937305,
-0.0018110029632225633,
-0.07453928142786026,
0.001959471497684717,
0.0020464672707021236,
0.024003582075238228,
-0.025013742968440056,
0.014671053737401962,
-0.0032176170498132706,
0.048367854207754135,
-0.016244463622570038,
0.06880789250135422,
-0.009505946189165115,
0.004697456955909729,
-0.0518951453268528,
-0.009118430316448212,
0.005397479515522718,
0.01743779145181179,
-0.003302356693893671,
-0.03181900829076767,
-0.1772879958152771,
-0.005937784444540739,
-0.052163511514663696,
0.00021581182954832911,
-0.037895701825618744,
-0.012816800735890865,
-0.02455291710793972,
0.012914320454001427,
-0.07253338396549225,
-0.05754689872264862,
0.040088679641485214,
0.015171115286648273,
0.014443504624068737,
0.01206680666655302,
0.0066829524002969265,
-0.032355066388845444,
0.02055211551487446,
-0.022662702947854996,
0.006665830500423908,
0.09627902507781982,
-0.007465509232133627,
-0.029118221253156662,
-0.036263357847929,
0.02611907199025154,
0.017576759681105614,
-0.0018999414751306176,
-0.022622115910053253,
0.034541402012109756,
0.025867443531751633,
-0.009143243543803692,
0.0030680883210152388,
0.01658656820654869,
0.04577457532286644,
-0.14121970534324646,
0.019658010452985764,
0.0219487976282835,
-0.034216590225696564,
-0.03399668261408806,
-0.005178099498152733,
0.02940554916858673,
0.03510793298482895,
-0.04632076248526573,
0.04996239021420479,
0.03725312650203705,
0.06925851106643677,
0.03736274316906929,
-0.0347415991127491,
-0.04094894230365753,
-0.026742368936538696,
-0.013211455196142197,
0.004897657781839371,
-0.04156043380498886,
0.026361515745520592,
-0.04866524785757065,
-0.045804768800735474,
-0.04872582480311394,
-0.04722249507904053,
0.05580176040530205,
-0.036066435277462006,
0.015986522659659386,
0.018005887046456337,
-0.001505498425103724,
-0.038386013358831406,
-0.004652868956327438,
-0.04717081040143967,
-0.04586678743362427,
-0.029823319986462593,
-0.014520671218633652,
0.02466762252151966,
0.051637206226587296,
0.4254864454269409,
-0.04153323173522949,
-0.04738195240497589,
-0.019026491791009903,
0.020959939807653427,
0.024498362094163895,
0.0028910513501614332,
0.030390363186597824,
-0.0006825343589298427,
0.01887650601565838,
0.03714124485850334,
0.04940260201692581,
-0.012186814099550247,
0.05167652294039726,
-0.012797093018889427,
-0.014480114914476871,
-0.008755570277571678,
0.07646418362855911,
0.028918523341417313,
-0.06239701807498932,
0.04055806249380112,
0.017097022384405136,
-0.002269885502755642,
0.043980278074741364,
-0.008122730068862438,
0.06479448080062866,
-0.026720795780420303,
-0.01844988577067852,
0.09275169670581818,
0.013647008687257767,
0.05537267029285431,
0.007859275676310062,
0.004977703094482422,
-0.010919386520981789,
-0.013185818679630756,
0.03581974282860756,
-0.01733260601758957,
0.02793893963098526,
-0.038121242076158524,
0.003730901051312685,
-0.01888609491288662,
0.011627566069364548,
-0.05436280369758606,
-0.004612480755895376,
-0.06854435801506042,
-0.024018293246626854,
0.08163682371377945,
-0.06970629096031189,
0.061743587255477905,
-0.08655907958745956,
0.0010391318937763572,
-0.024818746373057365,
0.04403255507349968,
-0.04389713332056999,
0.011564845219254494,
0.029119687154889107,
-0.011307226493954659,
0.00014927287702448666,
-0.015712199732661247,
-0.03142937645316124,
0.045601699501276016,
0.005340323783457279,
0.01343904621899128,
0.042191535234451294,
0.06174233928322792,
-0.007315133698284626,
-0.02044968493282795,
-0.01688472554087639,
-0.010560792870819569,
0.030140239745378494,
0.013088123872876167,
-0.005458945874124765,
0.01467165444046259,
-0.0039711506105959415,
-0.0420762374997139,
-0.05308903753757477,
0.009583234786987305,
0.004926174413412809,
0.016409099102020264,
0.05190059170126915,
0.05561326444149017,
0.0481589213013649,
0.033352743834257126,
-0.010142362676560879,
0.03400898352265358,
-0.027124734595417976,
-0.015030723065137863,
-0.0625874474644661,
-0.015139070339500904,
0.023592859506607056,
0.06296294927597046,
-0.04535758122801781,
-0.00041449410491622984,
-0.1021447703242302,
0.026998311281204224,
-0.010802817530930042,
0.001483272877521813,
-0.03492118790745735,
0.0889117419719696,
0.003093231236562133,
-0.058878086507320404,
0.036714985966682434,
0.011599194258451462,
-0.028638245537877083,
0.008769067004323006,
0.03363342955708504,
0.026611896231770515,
0.06055551767349243,
-0.01870318502187729,
-0.011235383339226246,
0.020834947004914284,
-0.05606876313686371,
-0.01753349043428898,
0.008628304116427898,
0.027657171711325645,
-0.06266177445650101,
0.0706552192568779,
-0.014048142358660698,
-0.0002601135347504169,
0.02936437912285328,
0.0198697280138731,
0.05377652868628502,
-0.01090506836771965,
-0.04955420270562172,
-0.29105880856513977,
0.01526267547160387,
0.003931308630853891,
-0.08146635442972183,
0.08624923974275589,
-0.04418163374066353,
-0.0005067458841949701,
-0.03804614022374153,
-0.030207954347133636,
-0.06151672825217247,
0.0801854282617569,
0.039803870022296906,
-0.04095036908984184,
-0.0016142437234520912,
0.004408619366586208,
0.032808855175971985,
0.0361524373292923,
-0.021192027255892754,
-0.006642183754593134,
0.05505285784602165,
0.04219629243016243,
-0.0358298160135746,
0.023335885256528854,
0.04026677459478378,
0.011420327238738537,
-0.0357043594121933,
0.09745267033576965,
0.03647594898939133,
0.05638887360692024,
-0.03044101782143116,
-0.03858945891261101,
0.019944388419389725,
-0.03789149969816208,
-0.06541786342859268,
-0.04856419935822487,
-0.041522663086652756,
0.04648168757557869,
-0.010557164438068867,
0.030569851398468018,
-0.006800525356084108,
-0.026284491643309593,
0.06867040693759918,
-0.013647913932800293,
-0.0702921450138092,
0.00885823369026184,
-0.07247432321310043,
-0.01563800312578678,
0.062420155853033066,
0.008387750014662743,
0.050010185688734055,
-0.07638897746801376,
-0.01934569701552391,
0.0398801825940609,
0.01081406231969595,
-0.0015027852496132255,
-0.04680188372731209,
-0.024486742913722992,
-0.010215356945991516,
-0.059292975813150406,
0.025573618710041046,
-0.00889773853123188,
-0.024954432621598244,
0.04441068321466446,
-0.0023004617542028427,
-0.03890341520309448,
0.030738862231373787,
-0.01468646340072155,
-0.03959286957979202,
0.08632545918226242,
-0.03572911024093628,
-0.017979802563786507,
0.060778629034757614,
0.03067691996693611,
0.01631803624331951,
0.014878361485898495,
0.016164984554052353,
-0.013406313955783844,
0.008573104627430439,
-0.06899657845497131,
-0.0195886492729187,
0.061842139810323715,
-0.000029758144592051394,
0.035696953535079956,
0.023213565349578857,
0.0160724725574255,
0.02229624055325985,
-0.02640388533473015,
-0.03407103195786476,
0.03979778662323952,
0.07786945253610611,
0.011551744304597378,
0.03657066076993942,
-0.023962251842021942,
-0.0022818411234766245,
0.04826388880610466,
-0.0284423828125,
-0.3096316456794739,
-0.01830689236521721,
0.0747729167342186,
0.05027398094534874,
-0.00903130043298006,
0.026359232142567635,
0.05964704975485802,
-0.04581441730260849,
-0.00910588726401329,
0.045417819172143936,
-0.01590743474662304,
-0.024584805592894554,
0.05617832392454147,
0.054515331983566284,
-0.015905840322375298,
0.004820873495191336,
-0.020523685961961746,
-0.04337484389543533,
0.017276721075177193,
-0.05797936022281647,
0.06775128841400146,
-0.0655880719423294,
0.1286153495311737,
0.03364674746990204,
-0.03135140985250473,
0.025836816057562828,
0.06115473434329033,
0.07562638819217682,
-0.018970461562275887,
0.020983045920729637,
0.03479637950658798,
-0.039551202207803726,
0.05724640563130379,
0.0012366388691589236,
0.05673849210143089,
-0.008078284561634064,
0.028975632041692734,
-0.008228948339819908,
0.014891408383846283,
-0.044608257710933685,
-0.06168914586305618,
-0.06374333798885345,
-0.05905165150761604,
-0.012175279669463634,
0.05118120461702347,
-0.06964051723480225,
-0.019099963828921318,
-0.10734307765960693,
-0.0020088229794055223,
0.012650534510612488,
0.003178159473463893,
0.00901172123849392,
0.060475703328847885,
0.011099021881818771,
-0.04145198315382004,
0.04345335438847542,
0.004933053627610207,
-0.03552420064806938,
0.01119927130639553,
0.015576211735606194,
-0.013843955472111702,
-0.0078986044973135,
-0.029910370707511902,
0.08773455768823624,
-0.019392522051930428
] |
PMID:25046 | Simple bacterial preservation medium and its application to proficiency testing in water bacteriology. | A medium composed of nutrient broth, 1.8% boric acid, and 1% sodium chloride at pH 7.0 was shown to maintain the stability of Escherichia coli cultures for up to 10 days at room temperature. By using this preservation medium for preparing a simulated sample a successful proficiency test survey in water bacteriology was conducted. | Simple bacterial preservation medium and its application to proficiency testing in water bacteriology. A medium composed of nutrient broth, 1.8% boric acid, and 1% sodium chloride at pH 7.0 was shown to maintain the stability of Escherichia coli cultures for up to 10 days at room temperature. By using this preservation medium for preparing a simulated sample a successful proficiency test survey in water bacteriology was conducted. | [
-0.053329117596149445,
0.01971147209405899,
-0.04820596054196358,
-0.004094657022505999,
0.0887061208486557,
-0.024918189272284508,
-0.0298966895788908,
0.010333128273487091,
-0.061570532619953156,
-0.02756563015282154,
-0.05169461667537689,
-0.11505289375782013,
0.015084879472851753,
-0.01053044106811285,
-0.006704573053866625,
-0.03954076021909714,
0.03015577606856823,
-0.020470133051276207,
0.0034286025911569595,
0.005150517448782921,
0.030647577717900276,
0.004780770745128393,
-0.030815934762358665,
-0.01852467469871044,
0.017843054607510567,
0.00581962987780571,
0.017827920615673065,
-0.02801544964313507,
-0.012682309374213219,
-0.17605708539485931,
-0.032669395208358765,
-0.02913372591137886,
0.007463432382792234,
-0.003624184988439083,
-0.029931755736470222,
0.05181179568171501,
0.005197761580348015,
-0.05033935233950615,
-0.040403738617897034,
0.04070943593978882,
0.025641636922955513,
0.023551173508167267,
0.011798164807260036,
-0.005140694323927164,
-0.020967083051800728,
0.02229645662009716,
-0.013964970596134663,
0.015880106016993523,
0.09038139879703522,
-0.011498354375362396,
-0.02079620398581028,
-0.05449039116501808,
-0.03596065193414688,
0.0378035232424736,
-0.026399657130241394,
0.010110333561897278,
0.05201480910181999,
0.023843063041567802,
-0.02779165469110012,
-0.014473156072199345,
0.014441401697695255,
-0.0035005088429898024,
-0.11886290460824966,
0.04785308614373207,
-0.015228633768856525,
0.036491136997938156,
-0.03394478186964989,
0.012431398965418339,
0.05695642530918121,
0.017387721687555313,
-0.0531880185008049,
0.030137499794363976,
0.03092201240360737,
0.051007144153118134,
0.04323272779583931,
-0.002095069270581007,
0.03327202424407005,
-0.009110037237405777,
0.008069483563303947,
0.03587187081575394,
-0.01607571542263031,
0.023395298048853874,
0.05164288356900215,
0.013428956270217896,
-0.0354432687163353,
-0.03042762354016304,
0.03095368668437004,
0.002255883300676942,
0.009202784858644009,
0.03198730945587158,
-0.0193040631711483,
-0.05646929517388344,
0.01107612531632185,
0.041294533759355545,
-0.03175601363182068,
-0.04088935628533363,
-0.011850045993924141,
-0.022980626672506332,
-0.00014488310262095183,
0.3681804835796356,
-0.0679963082075119,
0.008379550650715828,
-0.007414358668029308,
-0.032763831317424774,
0.02939789369702339,
-0.03155568242073059,
0.008805235847830772,
0.0038726311177015305,
-0.014457483775913715,
0.002315135905519128,
-0.03131886199116707,
0.0028917943127453327,
0.03530837595462799,
-0.039904385805130005,
0.007529560476541519,
0.05440698564052582,
0.06460512429475784,
-0.014659617096185684,
-0.029389267787337303,
0.004340233281254768,
-0.002513444982469082,
0.001189522910863161,
0.07458747923374176,
-0.07207068800926208,
0.012239822186529636,
-0.03977178782224655,
0.016079196706414223,
0.0639021173119545,
-0.012147600762546062,
0.03405817598104477,
-0.004235764965415001,
-0.016169682145118713,
-0.009294340386986732,
-0.024665798991918564,
-0.025837447494268417,
0.022002439945936203,
0.025053229182958603,
-0.013970565982162952,
0.027164384722709656,
-0.07356208562850952,
-0.008631396107375622,
0.005580204539000988,
-0.002311734249815345,
-0.11042044311761856,
-0.07587385922670364,
0.0760028064250946,
-0.019195672124624252,
0.03764127939939499,
-0.03629232943058014,
-0.060276955366134644,
-0.03197233006358147,
0.06976466625928879,
0.00004318940045777708,
0.0063288272358477116,
0.05930778384208679,
-0.02349145896732807,
0.025347426533699036,
-0.02175017073750496,
-0.014586021192371845,
-0.037918198853731155,
-0.09925127029418945,
-0.047761160880327225,
-0.0014388469280675054,
0.08077964931726456,
-0.027072429656982422,
-0.004782071337103844,
0.01615985855460167,
0.01266754325479269,
0.0468304269015789,
0.004991874564439058,
0.04101971164345741,
0.01968115009367466,
0.036904823035001755,
0.0013424829812720418,
-0.01723932847380638,
-0.020559992641210556,
-0.006335906218737364,
0.042424701154232025,
0.04276234284043312,
-0.00506576057523489,
0.0034563487861305475,
0.005469032097607851,
-0.013864900916814804,
0.05289662256836891,
0.0434039868414402,
0.006947830319404602,
-0.06561540812253952,
-0.030338691547513008,
0.03606831282377243,
0.11563444882631302,
-0.022344134747982025,
-0.051013730466365814,
-0.05083375424146652,
0.04689963534474373,
0.01235971599817276,
0.033781539648771286,
-0.03184438869357109,
-0.003988053184002638,
0.03804744780063629,
-0.03294447436928749,
-0.016656048595905304,
0.022746816277503967,
-0.05083996057510376,
-0.012158065102994442,
0.05933382362127304,
0.0478111132979393,
0.08008607476949692,
-0.015132322907447815,
0.03681744262576103,
0.05011953040957451,
-0.09453505277633667,
-0.005532070063054562,
-0.07410683482885361,
-0.048946090042591095,
-0.013891356997191906,
0.011158575303852558,
0.029702192172408104,
-0.04067743197083473,
0.038325853645801544,
0.06226307898759842,
0.07130904495716095,
-0.05133283883333206,
-0.062987320125103,
-0.3170352578163147,
0.04369049891829491,
-0.023848293349146843,
-0.012234278954565525,
0.1960568130016327,
-0.07212992757558823,
0.012590563856065273,
-0.05611501634120941,
-0.022118479013442993,
-0.06419572979211807,
0.05249035358428955,
0.007859879173338413,
-0.026989977806806564,
-0.04938735067844391,
-0.06122048944234848,
0.0033912190701812506,
0.08535229414701462,
-0.03614084795117378,
0.056605130434036255,
0.01579289697110653,
-0.00035951222525909543,
0.006624754052609205,
0.015877138823270798,
0.03833569958806038,
0.06191779673099518,
-0.0015073565300554037,
0.10667228698730469,
-0.05950494483113289,
0.0264978539198637,
0.015540944412350655,
0.0202272180467844,
0.01830163039267063,
-0.029099134728312492,
-0.09859278798103333,
-0.03032337687909603,
0.003055885899811983,
-0.05243799090385437,
0.03032069467008114,
-0.009299208410084248,
0.007106584496796131,
0.02612980827689171,
0.04066145792603493,
0.00453938590362668,
-0.09299387037754059,
-0.01443210057914257,
-0.06352332979440689,
0.0415707565844059,
0.06752233952283859,
-0.05632789060473442,
-0.024212496355175972,
-0.004725690931081772,
-0.008921418339014053,
0.008962302468717098,
0.04848579689860344,
-0.017033707350492477,
0.04536563158035278,
-0.0020186463370919228,
0.0063497889786958694,
-0.042209915816783905,
-0.04647089168429375,
0.025492340326309204,
0.026476867496967316,
0.055856261402368546,
0.04748588800430298,
-0.0063850064761936665,
-0.01727188751101494,
0.0263360682874918,
-0.015473747625946999,
0.0606091134250164,
-0.04995466768741608,
-0.022129617631435394,
0.0370224229991436,
-0.011417732574045658,
-0.011913693509995937,
0.02577577531337738,
0.0484645739197731,
-0.04546550661325455,
-0.04111093655228615,
-0.04497072100639343,
-0.027818908914923668,
0.050116751343011856,
-0.012258478440344334,
0.026853526011109352,
-0.01313391700387001,
0.07324210554361343,
0.06608333438634872,
-0.001774621196091175,
-0.01080684456974268,
0.06963779777288437,
0.07719624787569046,
0.014765920117497444,
-0.002973372582346201,
-0.015229793265461922,
-0.04322890192270279,
0.008261637762188911,
-0.017289265990257263,
-0.2840884327888489,
0.04432769492268562,
0.03532887622714043,
-0.02407093159854412,
-0.03816588222980499,
0.028699373826384544,
-0.011272584088146687,
-0.009586985222995281,
-0.05783557519316673,
0.036304183304309845,
-0.0037868963554501534,
-0.01837083138525486,
0.06009896844625473,
-0.0160926915705204,
0.03254073113203049,
0.01899353414773941,
-0.003930905368179083,
-0.06548212468624115,
0.028781350702047348,
-0.0076261647045612335,
0.004339274019002914,
-0.0504186786711216,
0.13403287529945374,
0.02192297950387001,
0.0130333062261343,
0.018884768709540367,
0.06004563346505165,
0.05635325610637665,
0.006602039560675621,
0.0036821041721850634,
0.036900583654642105,
0.022294998168945312,
0.08250994980335236,
-0.04129301384091377,
-0.002821890404447913,
0.0241146981716156,
-0.024884281679987907,
-0.0207442007958889,
-0.019109362736344337,
-0.02121204137802124,
-0.051698237657547,
-0.007758026942610741,
-0.06599198281764984,
-0.01724541001021862,
0.02132965438067913,
-0.01580086536705494,
0.020528847351670265,
-0.0776880607008934,
-0.01651456579566002,
-0.038378022611141205,
-0.05211598053574562,
0.041846130043268204,
0.07946401834487915,
-0.015589732676744461,
0.015551873482763767,
0.037959907203912735,
0.03009067475795746,
0.011609901674091816,
-0.03318769857287407,
-0.0015593318967148662,
0.06540925055742264,
-0.022774863988161087,
-0.008967449888586998,
0.09990159422159195,
-0.016182908788323402
] |
PMID:25047 | Reduction of azo dyes by intestinal anaerobes. | Reduction of seven azo dyes (amaranth, Ponceau SX, Allura Red, Sunset Yellow, tartrazine, Orange II, and methyl orange) was carried out by cell suspensions of predominant intestinal anaerobes. It was optimal at pH 7.4 in 0.4 M phosphate buffer and inhibited by glucose. Flavin mononucleotide caused a marked enhancement of azo reduction by Bacteroides thetaiotaomicron. Other electron carriers, e.g., methyl viologen, benzyl viologen, phenosafranin, neutral red, crystal violet, flavin adenine dinucleotide, menadione, and Janus Green B can replace flavin mononucleotide. These data suggest that an extracellular shuttle is required for azo reduction. | Reduction of azo dyes by intestinal anaerobes. Reduction of seven azo dyes (amaranth, Ponceau SX, Allura Red, Sunset Yellow, tartrazine, Orange II, and methyl orange) was carried out by cell suspensions of predominant intestinal anaerobes. It was optimal at pH 7.4 in 0.4 M phosphate buffer and inhibited by glucose. Flavin mononucleotide caused a marked enhancement of azo reduction by Bacteroides thetaiotaomicron. Other electron carriers, e.g., methyl viologen, benzyl viologen, phenosafranin, neutral red, crystal violet, flavin adenine dinucleotide, menadione, and Janus Green B can replace flavin mononucleotide. These data suggest that an extracellular shuttle is required for azo reduction. | [
-0.03315003216266632,
0.023020360618829727,
-0.04448030889034271,
0.046854641288518906,
0.012621445581316948,
-0.01770354062318802,
0.037211813032627106,
0.10115540772676468,
-0.04231303185224533,
-0.05110951140522957,
0.013172872364521027,
-0.02764369547367096,
0.01904275454580784,
0.014973137527704239,
-0.03347419574856758,
-0.02724524959921837,
0.04986150935292244,
-0.005015397910028696,
-0.0927865207195282,
-0.037894826382398605,
0.0013879896141588688,
0.015834344550967216,
0.013499751687049866,
0.0258871428668499,
0.058812085539102554,
0.0007478278712369502,
0.0077094994485378265,
0.003035880858078599,
-0.061505258083343506,
-0.1874133050441742,
-0.003417887492105365,
-0.054725538939237595,
0.0517408512532711,
-0.00664149783551693,
-0.010631915181875229,
0.018785206601023674,
-0.03271358087658882,
-0.0050304061733186245,
-0.05663825199007988,
-0.015838734805583954,
0.0944971889257431,
0.025709377601742744,
-0.0035777268931269646,
0.032531172037124634,
-0.061047159135341644,
-0.006132062524557114,
-0.017308169975876808,
0.010318473912775517,
0.09466077387332916,
-0.025904137641191483,
0.025080395862460136,
-0.03767261281609535,
-0.003101233160123229,
0.04529140517115593,
-0.0000097053116405732,
-0.012023642659187317,
0.01654667779803276,
0.008215682581067085,
-0.04064752161502838,
0.00003577805182430893,
0.04441574960947037,
0.009911073371767998,
-0.15219226479530334,
0.056399114429950714,
0.05615448206663132,
-0.0345088392496109,
0.025318704545497894,
0.009020193479955196,
0.0295378677546978,
0.036909230053424835,
-0.08766183257102966,
-0.000298606842989102,
-0.039866335690021515,
-0.01776677556335926,
0.0037523764185607433,
-0.0003608172119129449,
0.06952326744794846,
-0.05236072093248367,
0.01858389750123024,
-0.08558861911296844,
-0.015045865438878536,
-0.01875627040863037,
-0.0359756201505661,
-0.006265285424888134,
0.065544493496418,
0.029426397755742073,
0.018064700067043304,
-0.007208098191767931,
0.003402515547350049,
0.032401371747255325,
0.06769894808530807,
-0.0560729019343853,
0.03418805077672005,
-0.01592326909303665,
-0.029354173690080643,
-0.007315241266041994,
-0.019324079155921936,
0.0027375861536711454,
0.032470058649778366,
0.3522542119026184,
-0.05405920371413231,
-0.011656713671982288,
-0.03874416649341583,
-0.07072275131940842,
-0.019016914069652557,
-0.05180041864514351,
0.01292380876839161,
-0.014217308722436428,
0.0005971633363515139,
0.05269817262887955,
0.017004508525133133,
0.004756358452141285,
0.05753662809729576,
-0.01457061618566513,
-0.045795656740665436,
0.07798437774181366,
0.01512986235320568,
0.0573030449450016,
-0.05310237035155296,
0.04794315621256828,
-0.038582492619752884,
-0.0416601337492466,
0.06819452345371246,
0.006011818069964647,
0.03515740483999252,
0.01652977429330349,
-0.054847024381160736,
0.08773406594991684,
0.03998459503054619,
0.03236345946788788,
0.039548199623823166,
-0.01496177352964878,
0.03150755539536476,
0.0038263273891061544,
0.028449643403291702,
0.048978280276060104,
0.07073193043470383,
-0.03535955771803856,
-0.024237459525465965,
-0.00766407884657383,
-0.025512775406241417,
-0.04635189473628998,
-0.034687403589487076,
-0.06580283492803574,
-0.06118480861186981,
0.04442233592271805,
-0.05476837232708931,
-0.03712008148431778,
0.019638508558273315,
-0.005666959565132856,
-0.008013212122023106,
0.05454912409186363,
-0.023702727630734444,
0.0404350720345974,
-0.053158678114414215,
0.09318719804286957,
-0.021126214414834976,
-0.004980330355465412,
-0.013568617403507233,
0.013220159336924553,
-0.0917978584766388,
0.01665280945599079,
0.014250541105866432,
0.06124643608927727,
-0.0383707657456398,
-0.04704322665929794,
-0.0136255556717515,
0.040430545806884766,
0.027799159288406372,
0.055620383471250534,
0.0035728097427636385,
-0.04687144607305527,
0.014312762767076492,
-0.02805027924478054,
-0.02501278929412365,
-0.0018211596179753542,
0.04607779532670975,
0.046019546687603,
0.04245947301387787,
-0.010056212544441223,
0.003041504882276058,
-0.01693294383585453,
0.03421537205576897,
-0.0032634371891617775,
0.05512797087430954,
0.010437778197228909,
-0.0038527329452335835,
0.02278708480298519,
-0.04365348070859909,
-0.00460693147033453,
-0.024172469973564148,
0.05066914111375809,
-0.1028391495347023,
0.05372816324234009,
0.00956482719630003,
-0.04573861509561539,
-0.05724602937698364,
0.0005236983997747302,
-0.05134760960936546,
-0.012388329952955246,
0.09835828840732574,
-0.004140444099903107,
0.006979994010180235,
0.04129600152373314,
0.0193204116076231,
0.039329711347818375,
0.022360360249876976,
0.019114429131150246,
-0.011257488280534744,
0.0013889723923057318,
-0.02935684286057949,
0.06754083931446075,
0.019890490919351578,
0.06039346754550934,
-0.041753094643354416,
0.03127530217170715,
-0.055848054587841034,
0.024142369627952576,
0.01568165235221386,
0.01302456296980381,
0.026493508368730545,
-0.063499316573143,
-0.03636721521615982,
-0.31455856561660767,
-0.004220036324113607,
0.02365814335644245,
0.010298194363713264,
0.062523752450943,
0.00797565933316946,
-0.03075689636170864,
-0.027742348611354828,
-0.008257435634732246,
-0.018450982868671417,
0.014329351484775543,
0.07114233076572418,
-0.05431381240487099,
-0.0224365945905447,
-0.004179137293249369,
0.023179367184638977,
0.048045892268419266,
-0.045500122010707855,
0.01812225580215454,
-0.016786620020866394,
0.01761055737733841,
-0.0040291412733495235,
0.04241447150707245,
-0.017896883189678192,
0.05648449435830116,
0.002952741226181388,
0.14369703829288483,
0.05808782950043678,
0.048699844628572464,
-0.05365205928683281,
0.022922053933143616,
0.02160402201116085,
-0.026775816455483437,
-0.05188056454062462,
0.0009278216166421771,
0.004307534545660019,
-0.03792976215481758,
-0.0644964650273323,
0.011521602049469948,
-0.024775786325335503,
0.01949271932244301,
0.07644890993833542,
0.008585689589381218,
-0.07988210767507553,
0.029316401109099388,
-0.03985678404569626,
0.003108089556917548,
-0.027914121747016907,
0.042822591960430145,
-0.03783348947763443,
0.027552952989935875,
0.06119690462946892,
0.02378239296376705,
0.024076176807284355,
0.03773459047079086,
-0.027561210095882416,
-0.0344432070851326,
0.02433195151388645,
-0.051292356103658676,
-0.021875478327274323,
0.0008624640177004039,
-0.012000802904367447,
0.041295360773801804,
0.022844549268484116,
0.004612138960510492,
0.035119011998176575,
-0.03753810003399849,
0.017429674044251442,
0.042609382420778275,
-0.02458464540541172,
0.03652375936508179,
0.1109505146741867,
-0.02452252246439457,
0.008711280301213264,
-0.0018551719840615988,
0.0050973002798855305,
0.014466071501374245,
-0.011560285463929176,
-0.08377793431282043,
-0.021462153643369675,
-0.025589443743228912,
0.03567251190543175,
0.07731896638870239,
0.006163927726447582,
-0.048413921147584915,
0.030136533081531525,
-0.0025868110824376345,
-0.019763648509979248,
0.06448166072368622,
0.03204043582081795,
0.0413801372051239,
-0.04333013668656349,
0.024871312081813812,
0.004515639040619135,
0.04416027292609215,
-0.011698824353516102,
-0.24064797163009644,
0.00967509113252163,
0.010874700732529163,
0.0028542079962790012,
-0.03324228525161743,
0.08637026697397232,
0.03231643885374069,
-0.08800430595874786,
0.007468050811439753,
0.008353871293365955,
-0.001161486143246293,
-0.01436194684356451,
0.02398841641843319,
0.03296602889895439,
0.01230321079492569,
-0.010603481903672218,
0.026598148047924042,
-0.007400512229651213,
-0.016282416880130768,
-0.062188949435949326,
0.03006073087453842,
-0.09093965590000153,
0.08230923861265182,
-0.013934456743299961,
-0.09509320557117462,
0.057863928377628326,
0.032984379678964615,
0.06753966957330704,
-0.048985887318849564,
0.0012768958695232868,
-0.038646843284368515,
0.03229629620909691,
0.06731319427490234,
-0.029816726222634315,
0.09529156982898712,
0.04719788581132889,
-0.03238361328840256,
-0.0016093639424070716,
0.007998062297701836,
-0.029172150418162346,
-0.07504257559776306,
-0.04611457139253616,
-0.11400020122528076,
-0.01089467853307724,
0.1047879159450531,
-0.05605471506714821,
-0.07368674874305725,
-0.06786853075027466,
0.023165803402662277,
0.024708572775125504,
-0.056647174060344696,
-0.017992667853832245,
-0.004101587925106287,
0.001975648570805788,
-0.019755065441131592,
-0.008363126777112484,
-0.09406288713216782,
-0.005461018532514572,
0.04055849090218544,
-0.008980645798146725,
0.031869132071733475,
-0.04406190291047096,
-0.06260048598051071,
0.06419732421636581,
0.00885973684489727
] |
PMID:25048 | Concentration of poliovirus from tap water onto membrane filters with aluminum chloride at ambient pH levels. | A method is described for the concentration of an enterovirus from large volumes of tap water by addition of small amounts of aluminum chloride to enhance virus removal by membrane filters. Tap water treated with 2 X 10(-5) M aluminum chloride showed a slight decrease in pH (less than 0.5), a slight increase in turbidity, and enhanced removal of poliovirus by membrane filters. Virus was quantitatively recovered by treating the filters with a basic buffer, and this eluate was reconcentrated to a small volume by adsorption to aluminum hydroxide flocs. Using these procedures, virus from 1,000 liters of water was reduced to a final eluate of 20 to 80 ml with a mean recovery of 70%. | Concentration of poliovirus from tap water onto membrane filters with aluminum chloride at ambient pH levels. A method is described for the concentration of an enterovirus from large volumes of tap water by addition of small amounts of aluminum chloride to enhance virus removal by membrane filters. Tap water treated with 2 X 10(-5) M aluminum chloride showed a slight decrease in pH (less than 0.5), a slight increase in turbidity, and enhanced removal of poliovirus by membrane filters. Virus was quantitatively recovered by treating the filters with a basic buffer, and this eluate was reconcentrated to a small volume by adsorption to aluminum hydroxide flocs. Using these procedures, virus from 1,000 liters of water was reduced to a final eluate of 20 to 80 ml with a mean recovery of 70%. | [
-0.022039931267499924,
-0.009132565930485725,
0.0677533969283104,
-0.03752046450972557,
-0.00006702336395392194,
-0.044782619923353195,
0.06620001792907715,
0.06283079087734222,
-0.03020733781158924,
-0.0009209996787831187,
-0.005650150123983622,
-0.07532154768705368,
0.06989466398954391,
-0.029349323362112045,
0.029326628893613815,
-0.043859586119651794,
0.04569900408387184,
-0.025727219879627228,
-0.0722350925207138,
0.03625695779919624,
0.021468939259648323,
-0.04333949089050293,
-0.001310261432081461,
-0.04208075627684593,
0.01161448284983635,
0.027001790702342987,
-0.016432158648967743,
0.002733631758019328,
-0.04827588424086571,
-0.18318980932235718,
-0.02424941211938858,
-0.022095907479524612,
-0.01358255185186863,
0.003997471183538437,
-0.042661167681217194,
0.023575471714138985,
-0.016324903815984726,
-0.04159555211663246,
-0.05256634205579758,
0.006213529501110315,
0.04140923172235489,
0.05804465711116791,
-0.03729815408587456,
-0.03147135302424431,
-0.08969610184431076,
-0.025968631729483604,
0.006523926742374897,
0.017609892413020134,
0.12685389816761017,
-0.016483476385474205,
-0.004222373478114605,
0.04055285453796387,
-0.0010200345423072577,
0.08263953030109406,
-0.04572970047593117,
-0.036385636776685715,
-0.0011849423171952367,
0.04433145374059677,
-0.0030417570378631353,
-0.006706717424094677,
0.016227371990680695,
0.007306438870728016,
-0.16595673561096191,
0.11679159849882126,
0.008034082129597664,
0.025692254304885864,
-0.03355393186211586,
0.0005767506081610918,
0.05583500489592552,
0.03932539001107216,
-0.08284715563058853,
0.06413768976926804,
-0.021277477964758873,
0.06481043249368668,
0.002822295995429158,
-0.04260161519050598,
-0.04445526748895645,
0.010012788698077202,
0.008113275282084942,
-0.03633633628487587,
-0.04010705649852753,
-0.01630168780684471,
0.013985523954033852,
-0.0438133142888546,
-0.006412862800061703,
0.06222622096538544,
0.011402077972888947,
-0.0073697627522051334,
0.04413837194442749,
0.04667423292994499,
-0.024609757587313652,
0.03145653009414673,
-0.011186400428414345,
0.013765000738203526,
-0.031666100025177,
0.04904686659574509,
-0.02542482502758503,
-0.006201704032719135,
0.014909799210727215,
0.40967416763305664,
-0.046324003487825394,
0.009821007028222084,
-0.006422131322324276,
-0.05670919269323349,
0.005890867672860622,
-0.012554268352687359,
-0.00517053110525012,
0.019352613016963005,
0.01609296351671219,
0.022792309522628784,
0.03652004525065422,
-0.0033304784446954727,
0.03333967179059982,
0.0018217028118669987,
-0.016660377383232117,
0.021257033571600914,
0.04768694192171097,
-0.032402731478214264,
-0.025217493996024132,
0.051213204860687256,
-0.030419420450925827,
-0.04677256941795349,
0.020873986184597015,
-0.013940423727035522,
0.08549907058477402,
0.03505683317780495,
0.018035389482975006,
0.1020055040717125,
0.021098505705595016,
0.05660014599561691,
0.00010934491729130968,
-0.04778611287474632,
0.004033973440527916,
-0.0014378338819369674,
0.046719685196876526,
-0.02307295612990856,
0.021960943937301636,
-0.04702172055840492,
0.005961084738373756,
-0.014970558695495129,
-0.005819106008857489,
-0.042154498398303986,
-0.05483225733041763,
-0.12332373857498169,
0.015897337347269058,
0.05187525972723961,
-0.04783305525779724,
0.05805163457989693,
-0.059409357607364655,
-0.10235518217086792,
0.012678801082074642,
0.08279390633106232,
-0.029233567416667938,
0.0018362668342888355,
0.048196941614151,
0.01970226876437664,
0.026232149451971054,
-0.015133772045373917,
0.012795435264706612,
0.033134013414382935,
-0.04446737840771675,
-0.026972182095050812,
-0.039500642567873,
0.04016420245170593,
0.01504887081682682,
0.015681998804211617,
-0.04800400510430336,
-0.024745970964431763,
0.0022601282689720392,
0.05934993550181389,
0.002485942328348756,
0.018228664994239807,
0.03515883535146713,
-0.001041883835569024,
-0.017560841515660286,
0.03203120827674866,
-0.05312735214829445,
-0.0016631578328087926,
0.007061526644974947,
0.008562537841498852,
-0.005561929661780596,
-0.02803531289100647,
-0.02415284514427185,
0.017164167016744614,
0.053622424602508545,
-0.026035111397504807,
0.004621962085366249,
0.031111281365156174,
0.006438049487769604,
0.03294745460152626,
-0.01665867492556572,
-0.013197999447584152,
0.0026540306862443686,
0.003608034225180745,
-0.0070503996685147285,
0.010112770833075047,
0.0036670654080808163,
-0.04986247420310974,
0.007513297256082296,
-0.04753660783171654,
0.02628854662179947,
-0.03979218751192093,
-0.04608677700161934,
0.04205431044101715,
-0.0023704979103058577,
0.06707097589969635,
0.012876258231699467,
0.023566842079162598,
0.09673038870096207,
0.06822419911623001,
-0.008410939015448093,
0.03191140294075012,
-0.046829674392938614,
-0.052610743790864944,
0.0009627350955270231,
-0.016839120537042618,
0.01774938963353634,
-0.03634709492325783,
0.024634789675474167,
-0.030468113720417023,
0.05596783384680748,
-0.015464558266103268,
-0.05820823833346367,
-0.2925603687763214,
-0.01943828910589218,
-0.01829678937792778,
-0.01706162467598915,
0.059558749198913574,
-0.11964602768421173,
0.015859294682741165,
-0.07322943955659866,
-0.0218715388327837,
-0.023459646850824356,
0.046032823622226715,
0.01148409303277731,
0.013181705959141254,
0.04033611714839935,
0.013104635290801525,
0.03592021018266678,
0.04705902561545372,
0.011534183286130428,
0.02204662188887596,
0.04353860765695572,
0.03587733581662178,
0.029097987338900566,
0.06465744972229004,
0.061653345823287964,
0.0596214197576046,
-0.07153578847646713,
0.12809593975543976,
0.009722271002829075,
0.0010978596983477473,
0.00238245353102684,
-0.021080486476421356,
0.018203238025307655,
0.049201637506484985,
-0.0353451706469059,
-0.0733204111456871,
0.012808692641556263,
-0.0408988818526268,
0.009780273772776127,
-0.05379878357052803,
-0.03402679041028023,
-0.01706078089773655,
0.0615944042801857,
-0.06919606029987335,
-0.02239108271896839,
0.007959422655403614,
-0.022423824295401573,
-0.011796729639172554,
0.05512005463242531,
-0.06762602180242538,
-0.010634277015924454,
0.0677688792347908,
-0.012121203355491161,
0.027225662022829056,
-0.015547380782663822,
0.0360499769449234,
-0.011728060431778431,
0.014081206172704697,
0.014196719974279404,
-0.048731107264757156,
-0.019108576700091362,
-0.04565419256687164,
-0.034221943467855453,
0.014519275166094303,
-0.0225322637706995,
-0.034577712416648865,
0.029084300622344017,
0.03360970690846443,
0.0009177280007861555,
0.019778449088335037,
0.036795713007450104,
-0.03967050835490227,
0.08934161812067032,
0.020291371271014214,
0.05847940593957901,
0.0050398507155478,
0.04197702556848526,
-0.026193704456090927,
-0.03694333881139755,
-0.062465302646160126,
-0.06415534764528275,
0.03156474977731705,
0.004429131746292114,
0.019174668937921524,
0.01944880746304989,
0.05411909148097038,
0.07227028161287308,
-0.039066627621650696,
0.018368948251008987,
-0.040405623614788055,
0.03027605079114437,
0.032890435308218,
-0.005586313549429178,
-0.049701567739248276,
0.03651471808552742,
0.030865157023072243,
0.021914025768637657,
-0.2796032130718231,
-0.025640908628702164,
0.01696871779859066,
0.011232087388634682,
-0.0013337184209376574,
0.0057974690571427345,
0.03954722732305527,
-0.030965572223067284,
0.03430017828941345,
0.011863909661769867,
-0.01707136631011963,
0.010570785962045193,
0.009042046032845974,
-0.02280351147055626,
0.020248042419552803,
-0.008343774825334549,
-0.032513294368982315,
-0.0457426942884922,
0.05411997810006142,
-0.0296284519135952,
-0.0002721503551583737,
-0.06837105005979538,
0.14961081743240356,
-0.02222300134599209,
-0.07081259042024612,
-0.00528247794136405,
-0.016020499169826508,
-0.011173670180141926,
0.001317742164246738,
-0.03814821317791939,
-0.007764624897390604,
0.011284071952104568,
0.06623566895723343,
-0.008731551468372345,
0.06466875970363617,
-0.004522697068750858,
-0.007067585829645395,
0.006488045211881399,
-0.01882009021937847,
-0.029216375201940536,
-0.029908617958426476,
0.025598885491490364,
-0.027448441833257675,
-0.01520104706287384,
-0.014840754680335522,
-0.013856454752385616,
-0.002767413854598999,
-0.08460292220115662,
0.017543956637382507,
0.05542297288775444,
-0.07165080308914185,
-0.02874303050339222,
0.058006737381219864,
-0.021601883694529533,
0.0170902032405138,
0.029838943853974342,
-0.031064562499523163,
0.006118445191532373,
0.008706041611731052,
0.03258057311177254,
0.07007256150245667,
-0.05084611475467682,
0.005384066142141819,
0.03588341549038887,
0.017628764733672142
] |
PMID:25053 | Hypotensive and bradycardic effects of classical alpha-sympathomimetic drugs upon intravenous administration to pentobarbitone-anaesthetized rats. | The effects of the classical alpha-sympathomimetic drugs naphazoline, tramazoline, xylometazoline, tetryzoline and oxymetazoline on blood pressure and heart rate of pentobarbitone-anaesthetized (75 mg/kg; i.p.) normotensive rats were studied following intravenous injections. With the aid of this experimental animal model a dose dependent decrease in mean arterial pressure and heart rate could be established for these agents, oxymetazoline excepted, for which only the bradycardia could be quantified. It is suggested that central alpha-adrenoceptors are involved in the mechanism of this cardiovascular inhibition in view of the most specific alpha-adrenoceptor-stimulating properties of these ddrugs. Additionally, the results indicate that the use of pentobarbitone as the anaesthetic enhances the hypotensive action of these agents, so that their pronounced peripheral vasopressive response can be overcome. | Hypotensive and bradycardic effects of classical alpha-sympathomimetic drugs upon intravenous administration to pentobarbitone-anaesthetized rats. The effects of the classical alpha-sympathomimetic drugs naphazoline, tramazoline, xylometazoline, tetryzoline and oxymetazoline on blood pressure and heart rate of pentobarbitone-anaesthetized (75 mg/kg; i.p.) normotensive rats were studied following intravenous injections. With the aid of this experimental animal model a dose dependent decrease in mean arterial pressure and heart rate could be established for these agents, oxymetazoline excepted, for which only the bradycardia could be quantified. It is suggested that central alpha-adrenoceptors are involved in the mechanism of this cardiovascular inhibition in view of the most specific alpha-adrenoceptor-stimulating properties of these ddrugs. Additionally, the results indicate that the use of pentobarbitone as the anaesthetic enhances the hypotensive action of these agents, so that their pronounced peripheral vasopressive response can be overcome. | [
-0.02783612720668316,
0.03347916901111603,
0.019943103194236755,
0.000198031120817177,
-0.02394549548625946,
0.01672276295721531,
0.027431853115558624,
0.029246753081679344,
-0.022501012310385704,
-0.05529710277915001,
-0.05060587823390961,
-0.009577680379152298,
-0.00681939534842968,
0.08694819360971451,
-0.04517168551683426,
-0.009123883210122585,
0.0271471980959177,
-0.0007271551294252276,
-0.043598245829343796,
0.020662358030676842,
-0.01929139904677868,
0.029825877398252487,
0.02762731909751892,
0.0023768185637891293,
-0.0042542642913758755,
0.02657111920416355,
0.020801620557904243,
0.01067924965173006,
-0.04980086162686348,
-0.16892215609550476,
-0.01263162400573492,
0.03536973148584366,
-0.008432677015662193,
-0.03988436609506607,
-0.02644777111709118,
0.013466255739331245,
-0.04726181924343109,
0.11493056267499924,
-0.011874169111251831,
0.012809116393327713,
0.03042355552315712,
0.035909295082092285,
0.003646975615993142,
0.04645543172955513,
-0.0024611155968159437,
-0.033606767654418945,
-0.023617330938577652,
0.011057334952056408,
0.10380586236715317,
-0.012089991942048073,
0.011050596833229065,
0.014031719416379929,
0.05559679493308067,
0.01776023954153061,
0.03076484054327011,
-0.04985096678137779,
-0.02721155248582363,
0.059140872210264206,
0.007297925651073456,
0.030298644676804543,
-0.014200952835381031,
0.02272043377161026,
-0.14946189522743225,
0.0548192523419857,
0.031030898913741112,
0.007934893481433392,
-0.062109414488077164,
-0.013015261851251125,
0.034109000116586685,
0.03633880615234375,
-0.00840374268591404,
0.024663709104061127,
0.029910869896411896,
-0.027861716225743294,
-0.013146346434950829,
-0.03608907014131546,
0.02603594958782196,
-0.07076690346002579,
-0.026503946632146835,
0.003042233642190695,
0.03782471641898155,
-0.04292406514286995,
-0.016041381284594536,
-0.02259214036166668,
0.035214342176914215,
-0.0402664877474308,
0.07849589735269547,
-0.07020293176174164,
-0.07175716757774353,
0.017540816217660904,
-0.04795617237687111,
-0.03965985029935837,
0.00090008758706972,
-0.04230908304452896,
-0.04842120036482811,
-0.021367134526371956,
-0.00460090721026063,
0.009389598853886127,
-0.03922351077198982,
0.37720927596092224,
0.042426008731126785,
0.027403099462389946,
-0.08889691531658173,
-0.03906037658452988,
0.04277776926755905,
-0.02562594972550869,
0.00887055043131113,
-0.040207698941230774,
-0.02200126275420189,
0.07215376943349838,
0.047480177134275436,
0.020703192800283432,
0.046477969735860825,
-0.03735525161027908,
0.016364360228180885,
-0.04411108046770096,
0.0262153297662735,
0.05496571585536003,
0.004919505212455988,
0.011968712322413921,
-0.038048479706048965,
-0.007180206477642059,
-0.016841035336256027,
0.03576112911105156,
0.018868479877710342,
-0.030290674418210983,
-0.04394076019525528,
0.06980517506599426,
0.03945315629243851,
0.00807102769613266,
0.08254518359899521,
-0.01691986247897148,
-0.0056656296364963055,
0.02010311372578144,
0.011126876808702946,
0.010357930324971676,
0.04168803244829178,
-0.02031552419066429,
0.014865017496049404,
-0.024786440655589104,
-0.05657905340194702,
-0.059965964406728745,
0.009582331404089928,
-0.1082054004073143,
-0.06011098250746727,
0.12275391817092896,
0.06293674558401108,
-0.012772157788276672,
-0.0537262037396431,
0.041799888014793396,
-0.028325863182544708,
0.022570433095097542,
-0.007616179529577494,
0.0887138769030571,
-0.041003406047821045,
0.0670144110918045,
-0.018157025799155235,
0.057530101388692856,
-0.010224718600511551,
0.03327370807528496,
-0.09629254043102264,
-0.027330806478857994,
-0.03166365623474121,
0.06529110670089722,
0.00645972928032279,
0.011366726830601692,
-0.03691175580024719,
0.018097026273608208,
-0.01141807995736599,
-0.013019404374063015,
-0.007390625309199095,
-0.003144193207845092,
-0.011511100456118584,
-0.035574767738580704,
-0.060782384127378464,
0.016115259379148483,
0.06908661872148514,
-0.04387945309281349,
-0.0050715128891170025,
-0.02164422906935215,
0.04888639226555824,
0.025855334475636482,
-0.00443746242672205,
0.012630946934223175,
0.020468158647418022,
0.07510556280612946,
-0.05112999677658081,
0.008584199473261833,
0.019384775310754776,
-0.014408263377845287,
-0.002018984640017152,
0.004200339317321777,
-0.09866615384817123,
0.017412740737199783,
-0.05167622119188309,
-0.01420167088508606,
-0.038359835743904114,
0.06057222932577133,
-0.03177961707115173,
-0.012002822943031788,
0.08373618870973587,
0.04446754232048988,
0.04556563124060631,
0.038062307983636856,
0.0010756972478702664,
0.0378836989402771,
0.029678920283913612,
-0.01837218925356865,
0.04374256730079651,
-0.03224833309650421,
-0.02993738278746605,
0.031161466613411903,
0.032471783459186554,
0.008294953033328056,
-0.04536854475736618,
0.05507300794124603,
-0.016675662249326706,
0.020874949172139168,
0.02489755116403103,
0.04174543544650078,
0.047192659229040146,
-0.00989595428109169,
0.02178248204290867,
-0.3332638144493103,
-0.03310761973261833,
0.004217874724417925,
-0.028023621067404747,
0.024304278194904327,
-0.050697825849056244,
0.05236677825450897,
-0.07045568525791168,
-0.020037231966853142,
-0.029146244749426842,
0.035650644451379776,
0.08515214920043945,
-0.02357705496251583,
-0.013148553669452667,
-0.018293263390660286,
0.03647368773818016,
0.0314599834382534,
-0.07228744775056839,
-0.014467556960880756,
0.021344266831874847,
-0.02610575407743454,
-0.004164454527199268,
-0.049233391880989075,
0.011074733920395374,
0.038110148161649704,
-0.023398054763674736,
0.09714372456073761,
-0.007820324040949345,
-0.06724704056978226,
-0.0007240013219416142,
0.005979193840175867,
0.04513528570532799,
0.007403506897389889,
-0.10344438254833221,
-0.006789261940866709,
-0.030233193188905716,
0.0772319957613945,
0.04119793698191643,
0.026988394558429718,
0.006249140482395887,
-0.08454445004463196,
0.037377938628196716,
0.013500247150659561,
-0.008337956853210926,
-0.07137922942638397,
0.01594509370625019,
0.017423419281840324,
0.007721613626927137,
-0.028455078601837158,
-0.05106126889586449,
-0.0032172813080251217,
0.03959318622946739,
0.011857538484036922,
-0.008379344828426838,
0.039768822491168976,
-0.04635987803339958,
-0.05904223397374153,
0.0008028274169191718,
-0.02392813190817833,
-0.05664981156587601,
0.023247793316841125,
0.05518515780568123,
0.05049556493759155,
-0.02705376036465168,
-0.04482979327440262,
0.0378207303583622,
0.013680870644748211,
0.045303646475076675,
0.0921667218208313,
0.031630389392375946,
-0.010427397675812244,
0.15065142512321472,
-0.016414819285273552,
0.008275515399873257,
0.05266498401761055,
-0.01426753681153059,
0.014246274717152119,
-0.013568324036896229,
-0.10106762498617172,
-0.03025093302130699,
-0.022103356197476387,
0.025858502835035324,
0.047169413417577744,
-0.00016912150022108108,
-0.05579160898923874,
-0.01297526154667139,
0.00687588332220912,
0.017415642738342285,
0.04153615981340408,
0.012646414339542389,
-0.029011517763137817,
-0.02145233005285263,
0.014442580752074718,
0.042304471135139465,
0.019966300576925278,
0.012737446464598179,
-0.2738696336746216,
-0.0012687034904956818,
0.02498798817396164,
-0.03723537549376488,
0.020062429830431938,
-0.04839608445763588,
0.014725946821272373,
-0.041744474321603775,
-0.02064523845911026,
-0.02460014447569847,
-0.037737295031547546,
0.08526799827814102,
0.043953921645879745,
0.05546202510595322,
-0.05790744721889496,
-0.026502594351768494,
0.015488043427467346,
0.007029186934232712,
0.026622744277119637,
-0.036268025636672974,
0.009278870187699795,
-0.008143567480146885,
0.12477713823318481,
0.00854271650314331,
-0.0020676730200648308,
0.03940641134977341,
0.010433086194097996,
0.012260804884135723,
0.011987692676484585,
-0.004360396880656481,
-0.01961442641913891,
-0.01622171141207218,
0.0890984982252121,
-0.033636633306741714,
0.031792737543582916,
0.010984795168042183,
-0.09511564671993256,
0.015138901770114899,
-0.03319691866636276,
-0.021490804851055145,
-0.03755384311079979,
0.02124055288732052,
-0.050632208585739136,
-0.016008393839001656,
0.05693512409925461,
-0.004827949218451977,
-0.05261300504207611,
-0.008829427883028984,
0.010813512839376926,
0.06097978726029396,
-0.044787317514419556,
-0.02961987443268299,
0.026104317978024483,
0.029855644330382347,
0.023885978385806084,
-0.039679188281297684,
-0.017013490200042725,
-0.01613200269639492,
0.008539851754903793,
-0.007496858946979046,
0.023183831945061684,
-0.007586468011140823,
-0.03487340733408928,
0.03850249946117401,
0.01132551021873951
] |
PMID:25054 | [Arterial malformations and Takayashu disease]. | The authors report a recent series of 5 cases with Takayashu's disease and discuss the common pathological presentation of malformations of the arterial trunk, caused by disorderd development of the arterial system or by later segmental arrested development of the large trunks. The disease, which presents in the young adult as a diffuse inflammatory process, is probably auto-immune, but has its foundations in embryonic and foetal development. | [Arterial malformations and Takayashu disease]. The authors report a recent series of 5 cases with Takayashu's disease and discuss the common pathological presentation of malformations of the arterial trunk, caused by disorderd development of the arterial system or by later segmental arrested development of the large trunks. The disease, which presents in the young adult as a diffuse inflammatory process, is probably auto-immune, but has its foundations in embryonic and foetal development. | [
-0.044312477111816406,
-0.046802736818790436,
0.009754534810781479,
0.03367936238646507,
0.0077419523149728775,
-0.000035373468563193455,
0.03956609591841698,
0.0675317570567131,
-0.005426633637398481,
-0.012780265882611275,
0.03431512415409088,
-0.06160847097635269,
0.019510559737682343,
0.04976344108581543,
-0.024559395387768745,
0.0034797717817127705,
-0.008737321011722088,
0.0023687374778091908,
-0.014509899541735649,
0.005014420952647924,
-0.05657501891255379,
0.01503153145313263,
0.014921500347554684,
-0.08246069401502609,
-0.01509079895913601,
0.02215718664228916,
0.06598233431577682,
-0.0020368292462080717,
0.010344520211219788,
-0.11139741539955139,
-0.04878133535385132,
-0.02407417818903923,
0.00660706777125597,
0.007128652185201645,
0.020833820104599,
-0.014263474382460117,
-0.051800768822431564,
0.054745543748140335,
-0.019159561023116112,
-0.02466767095029354,
0.025468485429883003,
0.06349309533834457,
0.04445548355579376,
0.017619967460632324,
0.03139011189341545,
0.04891597107052803,
0.013498724438250065,
-0.030236970633268356,
0.06666135787963867,
0.004476804751902819,
-0.05995146930217743,
-0.020641298964619637,
0.04349299892783165,
0.07937563210725784,
0.003974066115915775,
-0.014352627098560333,
0.03429659828543663,
0.012723302468657494,
0.0004563576076179743,
0.04142408072948456,
0.06604776531457901,
0.014733953401446342,
-0.06429135054349899,
0.07009152323007584,
-0.0065688579343259335,
0.0011301299091428518,
0.026161421090364456,
-0.0035176011733710766,
0.11803342401981354,
-0.020642314106225967,
0.006906561087816954,
-0.04082079976797104,
0.017816178500652313,
0.0690181702375412,
0.010969257913529873,
-0.03343959152698517,
-0.048453282564878464,
-0.07052582502365112,
-0.019861336797475815,
0.03892398253083229,
0.009740634821355343,
0.046646848320961,
-0.017098698765039444,
0.007284075021743774,
-0.03723687678575516,
-0.0007126932614482939,
0.012515120208263397,
-0.10578953474760056,
-0.10956809669733047,
0.019625961780548096,
-0.03374861925840378,
-0.0396292544901371,
0.04312749579548836,
0.0391240231692791,
-0.10511059314012527,
0.022906238213181496,
0.006829824764281511,
-0.04725571721792221,
-0.01866355538368225,
0.39857977628707886,
-0.015736613422632217,
-0.012157544493675232,
0.016001511365175247,
0.0007195889484137297,
-0.009183707647025585,
0.04894833639264107,
-0.0540587455034256,
-0.04302363842725754,
-0.021114598959684372,
0.04847795516252518,
0.007238320540636778,
-0.05434504151344299,
0.057574860751628876,
0.03051232174038887,
-0.05510319024324417,
-0.01738477684557438,
0.0019848779775202274,
-0.004713668487966061,
-0.11739178001880646,
0.06200934574007988,
0.03699744865298271,
0.007761990185827017,
0.02608856000006199,
-0.017626892775297165,
0.054810088127851486,
0.022883674129843712,
0.001362822251394391,
0.0638214722275734,
0.07757019996643066,
-0.032625313848257065,
0.03950829058885574,
0.05646457150578499,
-0.04463478922843933,
0.004601858090609312,
0.04298857972025871,
-0.008489107713103294,
-0.05174828693270683,
-0.020434098318219185,
-0.040557075291872025,
-0.030526848509907722,
-0.010553405620157719,
-0.0029491132590919733,
-0.02328994683921337,
-0.03319171071052551,
-0.0502699539065361,
0.08259967714548111,
-0.013981414027512074,
0.03071577660739422,
-0.04805272817611694,
0.04654283821582794,
0.002791033359244466,
-0.003071868559345603,
-0.028037402778863907,
0.016246838495135307,
0.015991834923624992,
0.015292419120669365,
-0.03095017559826374,
0.11098115891218185,
0.001240366487763822,
-0.016179582104086876,
-0.02460317872464657,
-0.034822795540094376,
-0.04588400572538376,
0.02972482144832611,
0.06397772580385208,
0.00829332321882248,
-0.06911155581474304,
0.01963741146028042,
0.051109857857227325,
-0.021743565797805786,
-0.05277551710605621,
0.0009471970261074603,
0.009003659710288048,
0.0013533488381654024,
0.06279689073562622,
0.0366259440779686,
-0.014799349941313267,
0.020586134865880013,
-0.020492497831583023,
0.029313480481505394,
0.08100250363349915,
0.020073985680937767,
0.013967695645987988,
0.05044393986463547,
0.02523919567465782,
-0.08452305942773819,
-0.04488122835755348,
-0.029135625809431076,
0.06051431596279144,
0.01658621057868004,
0.012724663130939007,
-0.03090565651655197,
-0.08820071816444397,
-0.047290485352277756,
-0.01445136871188879,
-0.05976473540067673,
-0.004822258837521076,
-0.022601056843996048,
0.026792388409376144,
-0.009581223130226135,
0.03584979102015495,
-0.015513763763010502,
-0.010877548716962337,
-0.026369456201791763,
-0.0166168250143528,
0.048880863934755325,
-0.04399309307336807,
-0.04513850808143616,
-0.059486836194992065,
0.0265748742967844,
-0.008486956357955933,
0.0731591135263443,
0.008750193752348423,
0.046662021428346634,
0.02949134260416031,
0.016080345958471298,
-0.046683184802532196,
-0.003015135880559683,
-0.007466637063771486,
0.015181221067905426,
0.08365601301193237,
-0.034930698573589325,
0.036151256412267685,
-0.321615606546402,
-0.046582773327827454,
-0.019328324124217033,
-0.08557215332984924,
-0.06231722980737686,
-0.00452030124142766,
-0.015058607794344425,
0.0021818792447447777,
0.025705944746732712,
0.0168483704328537,
0.005580885801464319,
0.07950060069561005,
0.01133323460817337,
-0.026132874190807343,
-0.022773567587137222,
0.07096684724092484,
0.00026152163627557456,
-0.03863779827952385,
-0.04741404578089714,
-0.016209280118346214,
0.0039191218093037605,
-0.005822575651109219,
0.007369589060544968,
0.030892645940184593,
0.005730161909013987,
0.02807462401688099,
0.1216447576880455,
0.02445351518690586,
0.025664085522294044,
-0.044461242854595184,
0.04738207906484604,
0.018612822517752647,
0.014699782244861126,
0.005237891338765621,
0.03135773539543152,
-0.08085650950670242,
-0.06489746272563934,
-0.05067194253206253,
-0.0785924643278122,
-0.03467283397912979,
-0.03475061058998108,
-0.06091175973415375,
-0.008977007120847702,
0.007237647660076618,
-0.030694512650370598,
-0.013570917770266533,
-0.0632133036851883,
-0.0064165024086833,
0.03772447258234024,
0.03533778339624405,
0.028755923733115196,
0.006900664418935776,
0.015113431960344315,
0.031971510499715805,
0.03022557497024536,
-0.02993430569767952,
-0.05362691357731819,
0.05123297870159149,
-0.05702972412109375,
-0.039271458983421326,
0.0107168173417449,
0.02512511797249317,
0.0067384736612439156,
-0.07399829477071762,
-0.04188757389783859,
0.06418353319168091,
0.012521355412900448,
0.014318139292299747,
0.02881215699017048,
0.01967054232954979,
-0.048237144947052,
0.11470834165811539,
-0.008585631847381592,
-0.0045748502016067505,
0.07794267684221268,
0.00702865794301033,
0.019686369225382805,
-0.03262319788336754,
-0.036862507462501526,
0.004898096434772015,
0.01562795229256153,
-0.09408681839704514,
0.03236982226371765,
0.05925220623612404,
-0.05587838590145111,
0.010896100662648678,
0.02355951815843582,
0.009074937552213669,
-0.02892470359802246,
-0.06242702156305313,
-0.0031831087544560432,
-0.02187233604490757,
-0.023197920992970467,
-0.03101697750389576,
-0.026795975863933563,
0.02076108567416668,
-0.25624120235443115,
-0.0011781472712755203,
-0.013387828134000301,
-0.01380068901926279,
0.039466533809900284,
-0.024705680087208748,
-0.07025553286075592,
-0.017315149307250977,
0.018795965239405632,
0.024205012246966362,
-0.0146551001816988,
0.0022392976097762585,
0.021046821027994156,
-0.021773099899291992,
-0.008564663119614124,
-0.01815066486597061,
0.03159157186746597,
-0.03896988555788994,
0.055570088326931,
-0.07028619199991226,
-0.03557895869016647,
0.007802102714776993,
0.14473114907741547,
0.035853803157806396,
-0.040540747344493866,
0.014090131036937237,
-0.002780065406113863,
0.02710825577378273,
-0.025472218170762062,
-0.005621754098683596,
-0.001627321937121451,
0.010210512205958366,
0.06801847368478775,
0.04132329672574997,
0.04815192148089409,
0.04560810327529907,
-0.04546312242746353,
0.0841507762670517,
-0.02843833714723587,
0.010276726447045803,
-0.008595946244895458,
0.003774473210796714,
-0.0709817185997963,
-0.01709304004907608,
0.06490624696016312,
0.061282847076654434,
0.007989645935595036,
-0.001965220784768462,
0.048820313066244125,
0.06224962696433067,
0.0029814441222697496,
0.03432406485080719,
-0.007551508489996195,
-0.01269219908863306,
0.04163975641131401,
0.012225487269461155,
0.027200348675251007,
-0.002790838247165084,
-0.00803518109023571,
-0.0004947770503349602,
0.0376402847468853,
-0.019150005653500557,
-0.11544812470674515,
0.0940956398844719,
0.02072758786380291
] |
PMID:25055 | [Action of a sodium lactate perfusion on the hemodynamic effects of beta-blockaders]. | In 15 dogs who had been given propranolol and 5 humans who had been given Pindolol, an infusion of sodium lactate produced: 1) an increased in blood lactate levels and pH; 2) an increase in the cardiac index; 3) a state in which an infusion of isoproterenol (inactive on its own) could act as it would have done had the betaadrenergic receptors not been blocked. A perfusion of lactic acid which was sufficient to cause the variations in pH and blood lactate levels that are seen with exercise had no action on the heart rate nor on the cardiac index of 10 dogs treated with propranolol and could not re-establish the betastimulating properties of isoproterenol. Finally, alkalosis produced by an infusion of THAM in 13 dogs or sodium bicarbonate in 2 humans, changed neither the cardiac index nor the heart rate, nor the response to isoproterenol after a betaadrenergic blockade. Infusion of sodium lactate associated with isoproterenol could be used to combat the depressent effects of betablockers in patients with cardiac disorders. | [Action of a sodium lactate perfusion on the hemodynamic effects of beta-blockaders]. In 15 dogs who had been given propranolol and 5 humans who had been given Pindolol, an infusion of sodium lactate produced: 1) an increased in blood lactate levels and pH; 2) an increase in the cardiac index; 3) a state in which an infusion of isoproterenol (inactive on its own) could act as it would have done had the betaadrenergic receptors not been blocked. A perfusion of lactic acid which was sufficient to cause the variations in pH and blood lactate levels that are seen with exercise had no action on the heart rate nor on the cardiac index of 10 dogs treated with propranolol and could not re-establish the betastimulating properties of isoproterenol. Finally, alkalosis produced by an infusion of THAM in 13 dogs or sodium bicarbonate in 2 humans, changed neither the cardiac index nor the heart rate, nor the response to isoproterenol after a betaadrenergic blockade. Infusion of sodium lactate associated with isoproterenol could be used to combat the depressent effects of betablockers in patients with cardiac disorders. | [
-0.08046811819076538,
0.005411435384303331,
-0.006819350644946098,
0.06011255085468292,
-0.02537999115884304,
-0.011454718187451363,
0.017867352813482285,
0.03372415155172348,
-0.05454254522919655,
-0.03315209224820137,
0.04038485139608383,
-0.02916824072599411,
-0.004143194295465946,
0.06904018670320511,
-0.047954011708498,
-0.016451358795166016,
0.03846902772784233,
-0.010787791572511196,
-0.064360611140728,
-0.053463373333215714,
-0.038260553032159805,
0.00403945054858923,
-0.028962209820747375,
-0.02939693070948124,
0.026398006826639175,
0.04893626272678375,
-0.026887163519859314,
0.04453499987721443,
-0.061288490891456604,
-0.17122262716293335,
0.015514724887907505,
-0.010410469956696033,
0.04122826084494591,
-0.0038725079502910376,
-0.03856801241636276,
0.04265382140874863,
-0.04135995730757713,
0.046716365963220596,
-0.004469216335564852,
0.058527275919914246,
0.020265020430088043,
-0.03269820660352707,
0.008373484015464783,
0.0592564195394516,
-0.012100151740014553,
0.020984791219234467,
-0.019800057634711266,
-0.009425222873687744,
0.11996035277843475,
0.003892297390848398,
0.004415465518832207,
0.023494653403759003,
0.005899298936128616,
0.01743219420313835,
0.014873086474835873,
-0.04764280095696449,
0.00386620732024312,
0.03068816103041172,
0.006044010631740093,
0.030081219971179962,
-0.0022669658064842224,
0.035152096301317215,
-0.1582680493593216,
0.06076479330658913,
0.012357803992927074,
0.07810990512371063,
-0.03695197403430939,
0.045596182346343994,
0.04331541433930397,
0.07012483477592468,
-0.019923193380236626,
0.01757579669356346,
0.024626467376947403,
-0.023745762184262276,
0.031990110874176025,
-0.00819240603595972,
-0.012314063496887684,
0.049942005425691605,
-0.04349038004875183,
-0.011064372956752777,
0.017231862992048264,
-0.04968676716089249,
-0.06335968524217606,
0.006329330150038004,
0.046767931431531906,
-0.05449998378753662,
0.10995927453041077,
-0.03194262832403183,
0.010724609717726707,
0.011537455022335052,
-0.06981830298900604,
-0.061030272394418716,
-0.04046633094549179,
0.014955265447497368,
-0.019339879974722862,
0.049044739454984665,
-0.04412728548049927,
0.008419767022132874,
-0.00046334631042554975,
0.34562283754348755,
0.037046343088150024,
0.052190400660037994,
-0.0602993369102478,
-0.02866092324256897,
0.010280217044055462,
-0.047203343361616135,
0.018440555781126022,
0.043284822255373,
-0.04500047490000725,
0.03486786410212517,
0.06789394468069077,
0.00453925970941782,
0.09199048578739166,
-0.045564617961645126,
-0.01924782618880272,
0.0076791890896856785,
0.053188506513834,
-0.015509972348809242,
-0.025158647447824478,
0.036808598786592484,
-0.03788474202156067,
0.02552226185798645,
-0.026179946959018707,
0.003136235289275646,
0.0462544746696949,
0.0054950653575360775,
0.013944648206233978,
0.06630989909172058,
0.06309682875871658,
0.0029779255855828524,
0.0550101101398468,
0.009330845437943935,
-0.0074741169810295105,
0.051920823752880096,
-0.012798711657524109,
-0.0784299299120903,
0.021513454616069794,
-0.02044854685664177,
0.09826765954494476,
-0.012678585015237331,
0.022077543660998344,
-0.03442046418786049,
0.009665366262197495,
-0.07553315907716751,
-0.061405718326568604,
0.05716126784682274,
0.03747016191482544,
-0.017231298610568047,
-0.08033264428377151,
0.005352861247956753,
-0.075070321559906,
0.006552161183208227,
-0.033808402717113495,
0.06950375437736511,
-0.04490255191922188,
-0.011928831227123737,
0.0065261488780379295,
0.058750543743371964,
-0.04381222277879715,
-0.02980874851346016,
-0.0617695190012455,
-0.06441168487071991,
-0.002798388246446848,
0.009760316461324692,
-0.012987280264496803,
0.06205856800079346,
-0.026850629597902298,
-0.0023713908158242702,
-0.008752561174333096,
0.028170282021164894,
-0.027830125764012337,
-0.03345947712659836,
0.026009446009993553,
-0.06686821579933167,
-0.02714727446436882,
0.0421069897711277,
0.01617717370390892,
-0.005128378514200449,
0.043913520872592926,
-0.05198904871940613,
0.01791328936815262,
0.010010105557739735,
0.043798137456178665,
0.0007712027872912586,
0.01004085410386324,
0.002854728838428855,
-0.06396166980266571,
-0.022943709045648575,
0.01978280022740364,
0.06541779637336731,
-0.012450057081878185,
0.019436463713645935,
-0.10014533996582031,
0.015775196254253387,
-0.024593334645032883,
0.0016112629091367126,
-0.048456817865371704,
-0.0003082230396103114,
-0.0799989253282547,
0.025473736226558685,
0.06450371444225311,
-0.004202363081276417,
0.0022928230464458466,
0.027048368006944656,
0.05107298493385315,
0.060213178396224976,
-0.04552718624472618,
0.014262882061302662,
0.07809976488351822,
0.02095038630068302,
0.028936060145497322,
0.08132270723581314,
0.008855740539729595,
0.001001926837489009,
-0.045515526086091995,
0.05344320833683014,
-0.041101451963186264,
-0.010746799409389496,
0.03442716225981712,
0.01685013435781002,
0.0778798758983612,
-0.0417371429502964,
0.022716406732797623,
-0.30881309509277344,
-0.054076991975307465,
-0.027536174282431602,
-0.008450033143162727,
0.02481094002723694,
-0.03431672230362892,
0.03882482647895813,
-0.04974690452218056,
-0.0635477751493454,
-0.04214243218302727,
-0.010488545522093773,
0.04296257346868515,
-0.003293238114565611,
0.007869531400501728,
0.022156061604619026,
0.050503604114055634,
0.0256307665258646,
-0.06881298869848251,
0.01837630197405815,
-0.019066737964749336,
0.0024091191589832306,
-0.04290575534105301,
0.029814740642905235,
0.006490868050605059,
0.057236213237047195,
0.015597371384501457,
0.09432000666856766,
-0.009512696415185928,
0.009402604773640633,
-0.039629869163036346,
-0.04315531998872757,
0.08201006799936295,
-0.00784275308251381,
-0.09551083296537399,
0.014589764177799225,
-0.09095825999975204,
0.04603700339794159,
0.016772452741861343,
0.04113791510462761,
-0.008902941830456257,
-0.07646351307630539,
0.02270178310573101,
-0.03557835519313812,
0.03581489995121956,
-0.03552721068263054,
0.021018125116825104,
0.02894657850265503,
0.02719062753021717,
-0.07162626087665558,
0.021483268588781357,
0.0005293712019920349,
-0.005611139349639416,
0.006851818412542343,
-0.0608610138297081,
0.08720595389604568,
-0.004289408680051565,
-0.0413866825401783,
-0.04532907158136368,
-0.014768296852707863,
-0.002281885128468275,
0.004167054779827595,
0.0027154681738466024,
0.009033157490193844,
-0.019003039225935936,
-0.05159595608711243,
0.008876972831785679,
0.00645647756755352,
0.0403720885515213,
0.042349446564912796,
0.029534639790654182,
0.003372523467987776,
0.1178760901093483,
-0.030534015968441963,
-0.02760794758796692,
0.09185447543859482,
0.021187977865338326,
-0.04989824816584587,
-0.04778620973229408,
-0.046682700514793396,
-0.008265181444585323,
-0.018760766834020615,
-0.058805447071790695,
0.023066043853759766,
0.021536901593208313,
0.006428583525121212,
-0.017606906592845917,
0.0037883601617068052,
0.027839167043566704,
0.049198612570762634,
0.012973897159099579,
-0.01095519121736288,
0.012009209021925926,
-0.003726518712937832,
0.012921818532049656,
-0.004407960921525955,
0.012833977118134499,
-0.23479190468788147,
-0.027384959161281586,
0.0690368041396141,
-0.0803784504532814,
0.044663429260253906,
-0.024062417447566986,
0.030658381059765816,
-0.03934427723288536,
-0.05457570403814316,
-0.010361106134951115,
-0.027681272476911545,
0.036073293536901474,
0.037942759692668915,
0.05614098161458969,
-0.012299995869398117,
0.00974653847515583,
0.029222138226032257,
-0.005723630078136921,
0.02802354097366333,
-0.07082483172416687,
0.012802660465240479,
0.028923338279128075,
0.15503552556037903,
0.07156970351934433,
-0.028043238446116447,
0.08574926108121872,
0.04820811375975609,
0.03918810933828354,
-0.036661747843027115,
0.04879489168524742,
-0.05741642042994499,
-0.005650979466736317,
0.056898266077041626,
-0.045754387974739075,
0.022740939632058144,
0.044826921075582504,
-0.06549793481826782,
-0.002775374334305525,
0.027161357924342155,
-0.004057479090988636,
-0.002921446692198515,
-0.015547579154372215,
-0.055766139179468155,
-0.05524458736181259,
0.04233837500214577,
-0.04017750918865204,
-0.07823652029037476,
-0.007675264496356249,
-0.030552733689546585,
0.019584117457270622,
-0.06573222577571869,
-0.04198611155152321,
0.04439293593168259,
0.009130525402724743,
0.0160067081451416,
0.002143421210348606,
-0.06644561141729355,
-0.019348762929439545,
-0.011231102980673313,
-0.026383748278021812,
0.0052391234785318375,
-0.03025803342461586,
-0.04526017978787422,
0.10165480524301529,
0.05609865114092827
] |
PMID:25056 | Bacterial 2,3-butanediol dehydrogenases. | Enterobacter aerogenes, Aeromonas hydrophila, Serratia marcescens and Staphylococcus aureus possessing L(+)-butanediol dehydrogenase produced mainly meso-butanediol and small amounts of optically active butanediol; Acetobacter suboxydans, Bacillus polymyxa and Erwinia carotovora containing D(-)-butanediol dehydrogenase produced more optically active butanediol than meso-butanediol. Resting and growing cells of these organisms oxidezed only one enantiomer of racemic butanediol. The D(-)-butanediol dehydrogenase from Bacillus polymyxa was partially purified (30-fold) with a specific activity of 24.5. Except NAD and NADH no other cofactors were required. Optimum pH-values for oxidation and reduction were pH 9 and pH 7, respectively. The optimum temperature was about 60 degrees C. The molecular weight was 100000 to 107000. The Km-values were 3.3 mM for D(-)butanediol, 6.25 mM for meso-butanediol, 0.53 mM for acetoin, 0.2 mM for NAD, 0.1 mM for NADH, 87 mM for diacetyl, 38 mM for 1,2-propanediol; 2,3-pentanedion was not a substrate for this enzyme. The L(+)butanediol dehydrogenase from Serratia marcescens was purified 57-fold (specific activity 22.3). Besides NAD or NADH no cofactors were required. The optimum value for oxidation was about pH9 and for reduction pH 4.5. The optimum temperature was 32-36 degrees C. The molecular weight was 100000 to 107000. The Km-values were 5 mM for meso-butanediol, 10 mM for racemic butanediol, 6.45 for acetoin, 1 mM for NAD, 0.25 mM for NADH, 2.08 mM for diacetyl, 16.7 mM for 2,3-pentanedion and 11.8 mM for 1,2-propanediol. | Bacterial 2,3-butanediol dehydrogenases. Enterobacter aerogenes, Aeromonas hydrophila, Serratia marcescens and Staphylococcus aureus possessing L(+)-butanediol dehydrogenase produced mainly meso-butanediol and small amounts of optically active butanediol; Acetobacter suboxydans, Bacillus polymyxa and Erwinia carotovora containing D(-)-butanediol dehydrogenase produced more optically active butanediol than meso-butanediol. Resting and growing cells of these organisms oxidezed only one enantiomer of racemic butanediol. The D(-)-butanediol dehydrogenase from Bacillus polymyxa was partially purified (30-fold) with a specific activity of 24.5. Except NAD and NADH no other cofactors were required. Optimum pH-values for oxidation and reduction were pH 9 and pH 7, respectively. The optimum temperature was about 60 degrees C. The molecular weight was 100000 to 107000. The Km-values were 3.3 mM for D(-)butanediol, 6.25 mM for meso-butanediol, 0.53 mM for acetoin, 0.2 mM for NAD, 0.1 mM for NADH, 87 mM for diacetyl, 38 mM for 1,2-propanediol; 2,3-pentanedion was not a substrate for this enzyme. The L(+)butanediol dehydrogenase from Serratia marcescens was purified 57-fold (specific activity 22.3). Besides NAD or NADH no cofactors were required. The optimum value for oxidation was about pH9 and for reduction pH 4.5. The optimum temperature was 32-36 degrees C. The molecular weight was 100000 to 107000. The Km-values were 5 mM for meso-butanediol, 10 mM for racemic butanediol, 6.45 for acetoin, 1 mM for NAD, 0.25 mM for NADH, 2.08 mM for diacetyl, 16.7 mM for 2,3-pentanedion and 11.8 mM for 1,2-propanediol. | [
-0.02997332438826561,
0.006019788794219494,
-0.03825446590781212,
0.035710085183382034,
0.043348513543605804,
-0.05440285801887512,
-0.043204184621572495,
0.046726565808057785,
-0.01637067273259163,
-0.04984351620078087,
0.002282975474372506,
-0.08007270097732544,
0.0336528904736042,
-0.11507446318864822,
0.005068945232778788,
-0.05072109028697014,
0.041811179369688034,
0.04006706923246384,
0.0005304107908159494,
-0.01879316382110119,
0.01592710241675377,
-0.007732840720564127,
-0.026571927592158318,
0.027271348983049393,
0.06878978759050369,
0.03636166825890541,
0.0092284781858325,
0.06480276584625244,
0.0057090348564088345,
-0.1999683529138565,
0.013792303390800953,
0.022997744381427765,
0.03048730455338955,
-0.06327132135629654,
0.01082299929112196,
-0.026280080899596214,
-0.0015571683179587126,
-0.025216851383447647,
-0.020245417952537537,
0.04147358238697052,
0.023352012038230896,
0.003528644796460867,
0.043023731559515,
-0.001416279817931354,
-0.04655109718441963,
-0.016843119636178017,
-0.05503994971513748,
0.022799694910645485,
0.0914018452167511,
0.011343587189912796,
0.034619275480508804,
0.012442730367183685,
0.0031540212221443653,
0.018897760659456253,
0.03869052976369858,
-0.0967838242650032,
0.052750952541828156,
-0.03461027890443802,
-0.015322808176279068,
-0.014376307837665081,
0.008621844463050365,
0.02285517193377018,
-0.11703161150217056,
0.0054696775041520596,
-0.011347886174917221,
-0.00832441821694374,
0.02816229686141014,
-0.0883142352104187,
0.038668833673000336,
0.03919010981917381,
-0.005528809502720833,
0.05301652103662491,
0.04859676584601402,
-0.036113984882831573,
0.026947613805532455,
0.0001254648668691516,
-0.016918445006012917,
-0.029651619493961334,
0.014835169538855553,
-0.02279377356171608,
-0.029944943264126778,
0.008128596469759941,
-0.033937934786081314,
-0.03562984615564346,
-0.014783168211579323,
0.029406871646642685,
0.05219998583197594,
-0.04185717552900314,
0.02732882834970951,
0.044887710362672806,
0.006908682640641928,
-0.05554977059364319,
0.000572957331314683,
-0.050053443759679794,
-0.061364803463220596,
0.024831989780068398,
0.030876830220222473,
0.023813989013433456,
0.004027829971164465,
0.39148151874542236,
-0.033528659492731094,
-0.0028929521795362234,
-0.028716204687952995,
-0.048101551830768585,
0.01652986742556095,
-0.006992862559854984,
-0.027760274708271027,
0.013866518624126911,
0.00956032332032919,
-0.005047151818871498,
0.004533305298537016,
0.0045364899560809135,
0.0033377015497535467,
-0.038121964782476425,
-0.00380692002363503,
0.0009750602184794843,
0.05055604875087738,
-0.01393937412649393,
-0.041563980281353,
0.058003488928079605,
-0.008816162124276161,
-0.01627529226243496,
0.05450811982154846,
0.026808269321918488,
0.002026349538937211,
-0.024449774995446205,
-0.04619915410876274,
0.0807090625166893,
0.04865287244319916,
0.04394150897860527,
0.027009615674614906,
-0.004028442315757275,
-0.012728809379041195,
0.02022489160299301,
0.0035961491521447897,
-0.09792798012495041,
0.061047978699207306,
-0.01568497158586979,
0.012134548276662827,
-0.046429991722106934,
-0.007357957307249308,
-0.011230820789933205,
-0.01887131854891777,
-0.05550770089030266,
-0.048169299960136414,
0.04760551080107689,
-0.04905842989683151,
-0.027364695444703102,
-0.038117337971925735,
-0.01350473240017891,
0.02880287915468216,
0.07519752532243729,
0.03828318789601326,
0.01787867210805416,
0.06816025078296661,
0.04748084023594856,
-0.02246982231736183,
-0.03759366646409035,
0.019337790086865425,
0.006807285360991955,
-0.038086410611867905,
-0.008073931559920311,
0.0716465637087822,
0.03877883777022362,
0.04726599529385567,
-0.03348471596837044,
-0.04750608652830124,
0.050830136984586716,
0.021491456776857376,
0.05168571695685387,
0.006408912129700184,
-0.01433694176375866,
-0.01734352298080921,
-0.026697387918829918,
-0.04662740230560303,
-0.0222951527684927,
0.01662560924887657,
0.08373473584651947,
0.007537785917520523,
0.04420030862092972,
0.029616976156830788,
-0.04543251916766167,
0.0008315996965393424,
0.022438453510403633,
0.03069762885570526,
-0.026987405493855476,
-0.047149159014225006,
-0.013837005011737347,
-0.02380378544330597,
0.020554766058921814,
-0.03654715046286583,
0.012438184581696987,
-0.08438495546579361,
0.03419690579175949,
-0.019318366423249245,
-0.01833028346300125,
-0.024373386055231094,
0.028687013313174248,
-0.015731588006019592,
-0.03887765109539032,
0.15617163479328156,
0.01709170825779438,
-0.015717610716819763,
0.006193370092660189,
0.015046661719679832,
0.022075114771723747,
0.00251892302185297,
0.0021410740446299314,
0.009636953473091125,
-0.01976071670651436,
-0.07840396463871002,
0.0023308235686272383,
-0.06068367511034012,
-0.05284103378653526,
-0.06385979801416397,
0.0022686454467475414,
-0.013046521693468094,
0.03659955412149429,
0.03577583283185959,
0.02151516079902649,
0.0835883691906929,
-0.024491706863045692,
0.01046060025691986,
-0.3318735659122467,
-0.02421785332262516,
-0.04261226952075958,
-0.059628959745168686,
0.05245364084839821,
-0.017580896615982056,
0.010894542559981346,
0.00338561600074172,
-0.0018774623749777675,
-0.010084684938192368,
0.05215463042259216,
0.006443881429731846,
0.0029664014000445604,
-0.025798358023166656,
-0.037835944443941116,
0.024849388748407364,
0.027562344446778297,
-0.08836303651332855,
-0.006670774891972542,
-0.0002040587569354102,
0.025176919996738434,
-0.00360594829544425,
-0.011878999881446362,
0.05575593188405037,
0.08643102645874023,
-0.02578129991889,
0.10217560827732086,
0.0018322046380490065,
0.042503394186496735,
-0.07418893277645111,
0.060892850160598755,
0.023573800921440125,
-0.05391418933868408,
0.0024670958518981934,
-0.03919944167137146,
-0.07586248964071274,
-0.0026255487464368343,
-0.08288003504276276,
0.01982385665178299,
0.020494915544986725,
0.018659867346286774,
0.03545835614204407,
0.0017361550126224756,
-0.07244309037923813,
0.010771496221423149,
-0.057944104075431824,
0.03483057767152786,
0.009310375899076462,
-0.024605171754956245,
-0.03172387182712555,
-0.050339844077825546,
0.05376918241381645,
0.0624033585190773,
-0.02924298122525215,
-0.021112387999892235,
-0.006080560851842165,
-0.04950127750635147,
0.024092691019177437,
-0.0473979152739048,
0.02532077021896839,
0.011285297572612762,
0.009643924422562122,
-0.017614735290408134,
0.054112836718559265,
-0.028305144980549812,
0.024514559656381607,
-0.04546019434928894,
-0.01170341670513153,
0.05817709490656853,
0.015820370987057686,
0.01430057268589735,
0.09316349029541016,
0.012839916162192822,
-0.010872462764382362,
0.06339849531650543,
0.03920518979430199,
-0.00560940383002162,
-0.024370133876800537,
-0.08127276599407196,
-0.02257021702826023,
-0.022840475663542747,
-0.017483802512288094,
0.06893802434206009,
-0.0350443460047245,
0.005331337917596102,
0.050045646727085114,
-0.015920553356409073,
-0.03464455530047417,
0.027462733909487724,
0.060184236615896225,
0.029730282723903656,
0.005182045511901379,
0.007404491305351257,
-0.03524523973464966,
-0.01070290245115757,
-0.017391355708241463,
-0.23604489862918854,
-0.04484318196773529,
0.030245574191212654,
0.045648977160453796,
-0.019456319510936737,
0.02957616001367569,
0.060436733067035675,
-0.042562272399663925,
0.020113522186875343,
-0.004758734721690416,
0.0013585907872766256,
0.02668999880552292,
0.006971721071749926,
0.06751282513141632,
-0.02963089942932129,
0.04554847627878189,
0.019333232194185257,
-0.050724804401397705,
0.027231648564338684,
0.02187519706785679,
-0.023749491199851036,
-0.027391213923692703,
0.12393441051244736,
0.023783838376402855,
-0.09067953377962112,
0.07351379841566086,
0.010173017159104347,
0.026364797726273537,
-0.060856081545352936,
0.03166252747178078,
0.007593683898448944,
0.003016670234501362,
0.1331470012664795,
-0.036270350217819214,
0.024974334985017776,
0.05995672568678856,
-0.031168542802333832,
-0.021521268412470818,
0.07376786321401596,
0.06383184343576431,
-0.03708353266119957,
-0.011049531400203705,
-0.06674464046955109,
-0.020203808322548866,
0.0820392370223999,
-0.04318416118621826,
-0.03933732956647873,
-0.047489847987890244,
-0.0006759679527021945,
0.04469766840338707,
-0.018214231356978416,
0.025363845750689507,
0.011552398093044758,
0.006116941571235657,
0.013529076240956783,
-0.011304928921163082,
-0.002588808536529541,
-0.05514739826321602,
-0.05154627934098244,
-0.0313415601849556,
-0.007853548973798752,
-0.02489648573100567,
-0.007171662990003824,
0.09947998821735382,
0.06916604191064835
] |
PMID:25059 | Significance of microbial contamination of stored cadaver kidneys. | The importance of microbial contamination of cadaver kidneys was assessed in 83 consecutively stored and transplanted kidneys. Fourteen kidneys had a single positive culture during storage and five had multiple positive cultures. Only one postoperative infection could be traced to kidney contamination during storage (Candida wound infection). In three of 64 patients who received noncontaminated kidneys, posttransplant wound infections developed. No wound infections occurred in 35 patients who received prophylactic antibiotics, whereas four wound infections occurred in 48 patients without antibiotic coverage. It is concluded that, although microbial contamination of stored cadaver kidneys occurs commonly, it is not an important source of infection in renal transplant recipients. | Significance of microbial contamination of stored cadaver kidneys. The importance of microbial contamination of cadaver kidneys was assessed in 83 consecutively stored and transplanted kidneys. Fourteen kidneys had a single positive culture during storage and five had multiple positive cultures. Only one postoperative infection could be traced to kidney contamination during storage (Candida wound infection). In three of 64 patients who received noncontaminated kidneys, posttransplant wound infections developed. No wound infections occurred in 35 patients who received prophylactic antibiotics, whereas four wound infections occurred in 48 patients without antibiotic coverage. It is concluded that, although microbial contamination of stored cadaver kidneys occurs commonly, it is not an important source of infection in renal transplant recipients. | [
0.022671690210700035,
0.029223890975117683,
0.05831032618880272,
-0.0016762498999014497,
0.008868097327649593,
-0.06439287960529327,
0.05463268607854843,
0.09705718606710434,
-0.026975484564900398,
0.05381835997104645,
-0.036462705582380295,
-0.0359075665473938,
0.009181389585137367,
-0.00913814827799797,
-0.07183573395013809,
-0.011121165938675404,
0.04512745141983032,
-0.0012437057448551059,
-0.07648005336523056,
0.018533920869231224,
0.035246748477220535,
-0.02456502430140972,
0.029187524691224098,
-0.019997186958789825,
0.013183781877160072,
-0.05134332552552223,
-0.0025688293389976025,
-0.013671609573066235,
-0.0097871208563447,
-0.17025400698184967,
-0.07386477291584015,
-0.06368542462587357,
-0.015854516997933388,
-0.03257115185260773,
0.0575515553355217,
-0.01917414180934429,
0.024515267461538315,
-0.01336588989943266,
-0.011735768988728523,
0.02386857569217682,
-0.005241469945758581,
0.04526432603597641,
-0.0187916848808527,
-0.006124116480350494,
-0.03339514508843422,
-0.03518544137477875,
0.027590790763497353,
-0.0013042647624388337,
0.12883561849594116,
-0.01199306920170784,
-0.021679135039448738,
-0.01899976097047329,
0.004247796721756458,
0.1162387952208519,
-0.018564745783805847,
-0.043272607028484344,
0.03677691891789436,
-0.013945871964097023,
-0.030417414382100105,
0.013607583940029144,
0.04675005003809929,
0.03316095098853111,
-0.09697093814611435,
0.03224245831370354,
-0.02958686649799347,
-0.01609840616583824,
-0.004952247254550457,
-0.03981456533074379,
0.02625732310116291,
-0.05179755389690399,
-0.08062665164470673,
0.0011652088724076748,
0.012725764885544777,
0.06176342815160751,
0.023046158254146576,
-0.04538536071777344,
0.04195653274655342,
0.006191742140799761,
-0.027545541524887085,
0.07412493973970413,
-0.025161098688840866,
0.03181282430887222,
0.05448893457651138,
0.011550617404282093,
-0.08086424320936203,
-0.04475630447268486,
0.05754031985998154,
-0.020927147939801216,
-0.013636471703648567,
0.016746342182159424,
0.031175198033452034,
0.039787616580724716,
-0.004511161707341671,
-0.011250056326389313,
-0.0539066456258297,
-0.021217962726950645,
0.030998611822724342,
0.032505519688129425,
-0.03256842494010925,
0.3948802649974823,
-0.035336703062057495,
-0.07383277267217636,
-0.048941031098365784,
0.008769992738962173,
0.03955443575978279,
-0.01737258769571781,
0.057114165276288986,
-0.03671633452177048,
0.006090179085731506,
-0.009545627981424332,
0.013551436364650726,
-0.026349494233727455,
0.04400729760527611,
-0.0004692631773650646,
-0.02087009698152542,
0.06569584459066391,
0.09583946317434311,
0.015165326185524464,
-0.016253823414444923,
0.0045335423201322556,
-0.003106707241386175,
0.022782308980822563,
0.04471566900610924,
-0.07056985795497894,
0.014821608550846577,
-0.007123700808733702,
0.07103782892227173,
0.057380564510822296,
0.010287255048751831,
0.009364469908177853,
0.02503664419054985,
0.003824021900072694,
0.018544279038906097,
0.02721276879310608,
0.012800347059965134,
-0.006415409501641989,
-0.021116122603416443,
-0.07968160510063171,
-0.010800869204103947,
-0.06396358460187912,
-0.0130955521017313,
-0.04427516087889671,
-0.05405468866229057,
-0.07503943890333176,
-0.07757914066314697,
0.08915766328573227,
0.01658676564693451,
0.030232442542910576,
-0.027612417936325073,
-0.04970383271574974,
0.0032810659613460302,
0.10434926301240921,
-0.009744429960846901,
0.013890661299228668,
0.00830612238496542,
-0.03401657193899155,
-0.014120037667453289,
-0.024302400648593903,
-0.006379888858646154,
0.01815282739698887,
0.006339427083730698,
-0.0625750869512558,
-0.021638553589582443,
0.06603281944990158,
0.019121630117297173,
0.0048473975621163845,
-0.022020474076271057,
-0.09369683265686035,
0.04508885741233826,
0.002144270809367299,
0.04629317298531532,
0.010300204157829285,
0.027555525302886963,
0.014706446789205074,
-0.030992697924375534,
0.0068859318271279335,
-0.0590948723256588,
-0.03368101269006729,
-0.024744698777794838,
-0.027707895264029503,
0.022355958819389343,
0.017902621999382973,
-0.022339750081300735,
0.06742910295724869,
0.04592803865671158,
-0.0005367310368455946,
-0.054448243230581284,
0.02102266065776348,
0.046623095870018005,
0.09679244458675385,
0.0018912688829004765,
-0.018622322008013725,
-0.01286731380969286,
-0.025328800082206726,
0.04292173683643341,
-0.004229934886097908,
-0.037455908954143524,
-0.008634775876998901,
-0.04306105524301529,
-0.01973697729408741,
0.015167318284511566,
-0.03811464458703995,
0.0009802019922062755,
0.012364048510789871,
-0.03492124006152153,
-0.00528950709849596,
0.03104550950229168,
0.008947011083364487,
0.024345137178897858,
0.0779077410697937,
-0.05300537496805191,
0.00882558524608612,
-0.0761551633477211,
-0.013625534251332283,
0.02826826646924019,
-0.0358198918402195,
0.009077843278646469,
-0.005032139830291271,
0.06076165288686752,
-0.014304812066257,
0.03928626328706741,
0.018439294770359993,
-0.010322134010493755,
-0.3099052906036377,
0.03632872924208641,
-0.034833043813705444,
0.013031546026468277,
0.030808530747890472,
-0.010630408301949501,
-0.007183486595749855,
0.008328194729983807,
-0.019928205758333206,
0.03207783028483391,
0.0979231745004654,
0.04218659549951553,
-0.02228987216949463,
0.0034811380319297314,
-0.012197844684123993,
0.045270875096321106,
-0.007286704145371914,
-0.03641118109226227,
-0.030902491882443428,
0.011303702369332314,
0.062057796865701675,
-0.007937608286738396,
0.031924329698085785,
-0.012237822636961937,
0.07945552468299866,
-0.08090048283338547,
0.09838145226240158,
-0.023410756140947342,
0.030423933640122414,
-0.03729468211531639,
0.009121383540332317,
0.007523677311837673,
-0.061602529138326645,
-0.11777987331151962,
0.03554127737879753,
0.005503256339579821,
-0.02066868543624878,
-0.01615646854043007,
0.012361522763967514,
0.047789983451366425,
-0.07504907250404358,
-0.005957417655736208,
-0.05321449041366577,
-0.026144063100218773,
0.015414870344102383,
-0.06352110207080841,
-0.04110923781991005,
0.06434349715709686,
-0.006099131423979998,
0.06942020356655121,
0.039060432463884354,
-0.008523372933268547,
0.026964208111166954,
0.039133090525865555,
0.018526924774050713,
-0.017012977972626686,
0.026497427374124527,
-0.05447850376367569,
-0.02091868221759796,
-0.014005188830196857,
-0.027696140110492706,
0.007186170667409897,
0.09458579123020172,
-0.034412529319524765,
-0.021258782595396042,
0.013123152777552605,
-0.061379749327898026,
0.061003316193819046,
0.05497471243143082,
-0.012148034758865833,
-0.008185720071196556,
0.1407904028892517,
-0.0062169963493943214,
0.011264428496360779,
0.03055732510983944,
0.03395623713731766,
-0.008637011982500553,
-0.01949227787554264,
-0.019404904916882515,
-0.02134796977043152,
0.025827737525105476,
-0.021531354635953903,
-0.005562799051403999,
0.003540724515914917,
0.06174348294734955,
0.091422438621521,
-0.054333388805389404,
0.0775887742638588,
0.03510531038045883,
-0.004768491722643375,
0.04060329869389534,
0.002368232933804393,
-0.03668767958879471,
-0.014141778461635113,
-0.030087025836110115,
0.007049494422972202,
-0.2515433728694916,
0.019235793501138687,
0.06715577095746994,
-0.007569471374154091,
-0.029142677783966064,
-0.01422689389437437,
-0.04977785050868988,
-0.02358868159353733,
0.07148221880197525,
0.03569918870925903,
0.04413289949297905,
0.0076083349995315075,
0.09319077432155609,
0.02411242201924324,
0.008396449498832226,
0.021513493731617928,
0.024942338466644287,
-0.051289401948451996,
-0.006318700034171343,
-0.02740863896906376,
0.014507316052913666,
-0.05717072635889053,
0.11757540702819824,
0.021059371531009674,
-0.02514886111021042,
-0.004141796380281448,
-0.003921080846339464,
0.08093991875648499,
-0.04468008503317833,
0.016561828553676605,
0.04751162976026535,
0.01214217022061348,
-0.012402796186506748,
0.017167314887046814,
0.02002617157995701,
0.0245340745896101,
-0.03871718794107437,
-0.0016049961559474468,
0.004031520336866379,
-0.004579873289912939,
-0.07370375841856003,
-0.013138416223227978,
-0.04750534147024155,
-0.02718012034893036,
0.050115931779146194,
-0.033704064786434174,
-0.00025539236958138645,
-0.09635163843631744,
0.034612227231264114,
-0.025726525112986565,
-0.040139585733413696,
0.030569586902856827,
0.012454140931367874,
-0.04707745090126991,
0.02151981182396412,
-0.011235615238547325,
-0.027432233095169067,
-0.06766277551651001,
-0.0009794530924409628,
0.042875632643699646,
0.0013476638123393059,
-0.009420067071914673,
-0.0751088559627533,
0.05455472692847252,
-0.022110702469944954
] |
PMID:25060 | The Grimonster symposium on the occasion of the 50th anniversary of the first lumbar sympathectomy. | Under the auspices of the Belgian Surgical Society, a small group of American and European surgeons discussed in detail the place of lumbar sympathectomy in present day surgical practice. A consensus was reached concerning the physiologic effect, anatomical variations and indications for the operation. Conclusions included the questionable value of lumbar sympathectomy for treating intermittent claudication due to aortoiliac disease alone, its possible effectiveness when obliterative arterial disease is limited to the femoral popliteal segment, and its occasional beneficial effect when performed in conjunction with some reconstructive arterial operations. | The Grimonster symposium on the occasion of the 50th anniversary of the first lumbar sympathectomy. Under the auspices of the Belgian Surgical Society, a small group of American and European surgeons discussed in detail the place of lumbar sympathectomy in present day surgical practice. A consensus was reached concerning the physiologic effect, anatomical variations and indications for the operation. Conclusions included the questionable value of lumbar sympathectomy for treating intermittent claudication due to aortoiliac disease alone, its possible effectiveness when obliterative arterial disease is limited to the femoral popliteal segment, and its occasional beneficial effect when performed in conjunction with some reconstructive arterial operations. | [
0.002930808113887906,
-0.020403722301125526,
0.04637046158313751,
-0.006013252306729555,
-0.01336304284632206,
-0.01726410910487175,
-0.01921534165740013,
0.07931847125291824,
0.0016116274055093527,
-0.031280457973480225,
0.02104935236275196,
-0.040210798382759094,
-0.004454705398529768,
0.05635831505060196,
-0.03845221921801567,
-0.022305825725197792,
0.023079851642251015,
0.05678178742527962,
0.015568375587463379,
0.03672689571976662,
-0.017940841615200043,
-0.038307592272758484,
0.048440881073474884,
-0.014436325058341026,
-0.0009750700555741787,
-0.007158671505749226,
0.01137580443173647,
-0.007124637719243765,
-0.043033283203840256,
-0.15521878004074097,
0.0034095854498445988,
-0.08682022988796234,
0.0010969951981678605,
0.022350573912262917,
-0.014515900053083897,
0.02485341578722,
-0.0583856925368309,
0.04327205941081047,
-0.09030833840370178,
0.02287987805902958,
0.06344188004732132,
-0.003711167722940445,
-0.022145822644233704,
0.006604340393096209,
0.02955271489918232,
-0.00045203047920949757,
0.0025252175983041525,
-0.012988924048841,
0.06687834858894348,
0.04491510987281799,
0.03710339963436127,
0.018786178901791573,
0.055087246000766754,
0.011431097984313965,
0.01911805383861065,
-0.029630238190293312,
-0.029127854853868484,
0.0055959513410925865,
-0.01091084536164999,
-0.01904108002781868,
0.08781961351633072,
0.059004683047533035,
-0.18507131934165955,
0.08687622845172882,
0.01223758328706026,
-0.028453975915908813,
-0.013835451565682888,
-0.046937938779592514,
0.057378124445676804,
-0.0002627500216476619,
0.003022102639079094,
0.04999446123838425,
0.05324457585811615,
0.009199523366987705,
0.03393058851361275,
-0.004898077342659235,
-0.020601989701390266,
0.002066912828013301,
0.005146035458892584,
0.042732786387205124,
0.03954373300075531,
0.053844258189201355,
-0.06941701471805573,
-0.02868795208632946,
0.036936040967702866,
-0.02825787104666233,
0.01725051924586296,
-0.05749465525150299,
-0.10088543593883514,
0.00101591891143471,
-0.03257650136947632,
-0.02845270000398159,
0.004395177122205496,
-0.02809210680425167,
-0.0435991995036602,
-0.015265114605426788,
-0.034626372158527374,
0.04813144728541374,
0.00487785367295146,
0.4175228476524353,
0.003087123390287161,
0.00768008828163147,
0.007107360754162073,
0.006974721793085337,
0.0753106102347374,
0.016347873955965042,
0.020609555765986443,
0.03426671400666237,
-0.001970043871551752,
0.06317011266946793,
0.09138373285531998,
-0.0249737948179245,
0.05209888890385628,
-0.014137297868728638,
-0.0440145768225193,
0.013251214288175106,
0.008806673809885979,
0.07042314857244492,
0.0046515092253685,
-0.009587947279214859,
-0.047658368945121765,
-0.08266390860080719,
0.04617683216929436,
0.004789732396602631,
0.01667436771094799,
-0.031093094497919083,
0.04113081097602844,
0.08799435943365097,
0.05956354737281799,
-0.005368946585804224,
0.06420136243104935,
-0.04559530317783356,
-0.019595663994550705,
-0.06427401304244995,
-0.014988962560892105,
-0.03804217278957367,
0.009648353792726994,
-0.031319279223680496,
-0.005386508535593748,
-0.07276895642280579,
0.06109407916665077,
-0.05319162458181381,
0.004732622765004635,
-0.04154084995388985,
-0.05695997551083565,
0.03984823450446129,
0.026576431468129158,
-0.028590677306056023,
-0.07557451725006104,
-0.0020358252804726362,
0.002891687210649252,
-0.02185756526887417,
-0.03924981504678726,
0.05009853467345238,
-0.010896064341068268,
-0.013987887650728226,
0.006093564443290234,
0.016782961785793304,
0.010307044722139835,
-0.01742713712155819,
-0.03778919577598572,
-0.04513351991772652,
-0.037183597683906555,
0.09659739583730698,
0.029648493975400925,
-0.030916227027773857,
-0.012444292195141315,
0.002546451287344098,
0.06252164393663406,
0.0014206347987055779,
0.02708684653043747,
-0.02048909291625023,
0.034294117242097855,
-0.02986428700387478,
0.014969268813729286,
0.029181580990552902,
0.03342274948954582,
-0.024670375511050224,
-0.03127093240618706,
-0.026121776551008224,
0.06331950426101685,
-0.00858276803046465,
0.04236382246017456,
-0.005821798928081989,
-0.013135762885212898,
-0.026147691532969475,
-0.05137128010392189,
-0.04158555343747139,
0.03405650705099106,
0.014616760425269604,
-0.015475515276193619,
-0.016887055709958076,
-0.10540593415498734,
-0.026641512289643288,
-0.003747738664969802,
-0.07633421570062637,
-0.06039958447217941,
-0.011776268482208252,
-0.08740872144699097,
0.0027846465818583965,
0.005627098958939314,
0.0017579393461346626,
-0.00880520697683096,
0.06562626361846924,
0.06219731271266937,
0.006038401275873184,
-0.01550358347594738,
-0.02576698176562786,
0.01067276205867529,
0.055437926203012466,
-0.011613469570875168,
0.06011971831321716,
0.07503930479288101,
0.020303012803196907,
-0.007442196365445852,
0.04452935978770256,
0.04497680813074112,
-0.023088663816452026,
0.025178108364343643,
-0.0634206086397171,
0.0258974377065897,
0.04085592180490494,
-0.007421649061143398,
-0.2971910238265991,
-0.03407618775963783,
-0.025640537962317467,
0.02542070485651493,
0.08839964121580124,
-0.042658619582653046,
-0.011169330216944218,
-0.0020733377896249294,
-0.04512440785765648,
-0.05803079158067703,
0.017681878060102463,
0.007078212685883045,
-0.023575391620397568,
-0.004233272280544043,
0.03725438937544823,
0.09280737489461899,
0.031186440959572792,
-0.04176686331629753,
-0.02781876176595688,
-0.007936959154903889,
0.016320860013365746,
0.03645231947302818,
0.013209489174187183,
0.04173237830400467,
0.014990439638495445,
-0.06727443635463715,
0.11453809589147568,
0.03717578575015068,
0.011295713484287262,
0.007109504193067551,
0.007330159656703472,
0.007873881608247757,
0.0037005594931542873,
-0.05196092650294304,
-0.021645480766892433,
-0.012907513417303562,
0.08666820079088211,
-0.029334383085370064,
0.00021130061941221356,
0.04965868964791298,
-0.06327817589044571,
0.03354441374540329,
-0.020186180248856544,
-0.01993161253631115,
-0.10559500753879547,
-0.033481113612651825,
-0.02420218102633953,
0.003160476218909025,
-0.04311203956604004,
0.027029240503907204,
0.02586507797241211,
-0.07408332824707031,
0.03864796459674835,
0.007924772799015045,
0.04359332099556923,
-0.0004344677727203816,
-0.05194045230746269,
-0.014696577563881874,
-0.002196950139477849,
-0.02195950411260128,
-0.028985586017370224,
-0.027409086003899574,
0.07811082154512405,
-0.08964850753545761,
-0.01998758874833584,
-0.029607901349663734,
0.053822316229343414,
0.02561040408909321,
0.10958269238471985,
-0.005134510342031717,
-0.05813894420862198,
0.043736618012189865,
-0.007760368753224611,
-0.05520898848772049,
0.019379237666726112,
0.01450388878583908,
0.03424319997429848,
0.07898859679698944,
-0.03419649973511696,
-0.04000715911388397,
0.039218734949827194,
-0.02303655631840229,
0.0018027195474132895,
0.015425754711031914,
0.009400494396686554,
-0.019028788432478905,
0.00736488401889801,
0.031296804547309875,
-0.016671637073159218,
-0.04839072376489639,
-0.005987938027828932,
0.007356203626841307,
-0.07759224623441696,
0.0639200508594513,
-0.013294967822730541,
-0.02815394103527069,
-0.2552497684955597,
-0.023132653906941414,
0.022993968799710274,
-0.039531003683805466,
0.009728638455271721,
-0.0017928853631019592,
-0.05950126424431801,
-0.040499571710824966,
0.012702453881502151,
0.011188315227627754,
0.09635332226753235,
-0.007754112593829632,
0.03124203160405159,
0.03379937261343002,
0.007203890476375818,
0.009781326167285442,
0.03420358896255493,
-0.055007342249155045,
-0.022729843854904175,
-0.10594018548727036,
-0.005217491649091244,
-0.0170576348900795,
0.09649383276700974,
0.010644367896020412,
0.013945371843874454,
0.0034557092003524303,
-0.05681486800312996,
0.015125254169106483,
-0.0251507256180048,
-0.017430484294891357,
0.009123007766902447,
-0.06166352331638336,
0.01936943642795086,
0.018494678661227226,
0.02538330852985382,
0.07990479469299316,
-0.04099438339471817,
0.03316153958439827,
0.01687796041369438,
0.010607581585645676,
0.026158619672060013,
-0.005481625907123089,
0.037467338144779205,
0.0003887742350343615,
0.11080583184957504,
0.03707217425107956,
-0.046388741582632065,
-0.019072769209742546,
-0.009565187618136406,
0.04360201209783554,
-0.009861884638667107,
0.009693820960819721,
0.017756715416908264,
-0.0013893700670450926,
-0.006997222546488047,
-0.010981456376612186,
-0.0045897457748651505,
-0.00006513103289762512,
0.036657772958278656,
0.01460614800453186,
-0.037329137325286865,
0.03248543292284012,
-0.04622534289956093,
0.054662007838487625,
0.003584302496165037
] |
PMID:25061 | Formation of 3,N4-ethenocytidine moieties in RNA by vinyl chloride metabolities in vitro and in vivo. | Rats were exposed to [1,2-14C] vinyl chloride. Liver RNA was isolated, hydrolyzed, and the nucleosides separated on Aminex-A-6. Besides the physiological bases and 1,N6-ethenoadenosine, radioactivity was also incorporated into 3,N4-ethenocytidine. Radioactive 3,N4-ethenocytidine moieties were also formed on incubation of polycytidylic acid with rat liver microsomes, NADPH and [14C] vinyl chloride. These alkylation mechanisms are consistent with the mutagenic and cancerogenic properties of vinyl chloride. | Formation of 3,N4-ethenocytidine moieties in RNA by vinyl chloride metabolities in vitro and in vivo. Rats were exposed to [1,2-14C] vinyl chloride. Liver RNA was isolated, hydrolyzed, and the nucleosides separated on Aminex-A-6. Besides the physiological bases and 1,N6-ethenoadenosine, radioactivity was also incorporated into 3,N4-ethenocytidine. Radioactive 3,N4-ethenocytidine moieties were also formed on incubation of polycytidylic acid with rat liver microsomes, NADPH and [14C] vinyl chloride. These alkylation mechanisms are consistent with the mutagenic and cancerogenic properties of vinyl chloride. | [
-0.03657000511884689,
0.0010614831699058414,
0.019407235085964203,
-0.009548529051244259,
0.011906751431524754,
0.001388655393384397,
0.05709901079535484,
-0.012992053292691708,
-0.09857673197984695,
0.02564934454858303,
0.03873133659362793,
-0.0028560052160173655,
-0.029964173212647438,
-0.020539917051792145,
0.006345709785819054,
-0.009619349613785744,
-0.03267771750688553,
-0.003517025150358677,
-0.06119713932275772,
0.01467800885438919,
-0.02230648882687092,
-0.0781630203127861,
0.0588076077401638,
0.05200541019439697,
0.03188057988882065,
0.03695069998502731,
-0.0027779138181358576,
-0.0033707087859511375,
-0.06956729292869568,
-0.20316317677497864,
0.025609586387872696,
0.03449711203575134,
0.020991656929254532,
-0.0379369780421257,
-0.021403122693300247,
-0.011124002747237682,
-0.051157500594854355,
0.010410480201244354,
-0.030871326103806496,
0.0396336205303669,
-0.018976500257849693,
0.022800877690315247,
-0.007220056839287281,
-0.02022414095699787,
0.007640273775905371,
0.03699827194213867,
0.0002074887161143124,
-0.007746923249214888,
0.09982536733150482,
0.017114048823714256,
0.03323451802134514,
-0.013018797151744366,
-0.04158256947994232,
-0.007849899120628834,
0.00982371810823679,
-0.031933367252349854,
0.036607805639505386,
0.0915563553571701,
-0.017150886356830597,
0.05375748872756958,
-0.002846864052116871,
0.048837557435035706,
-0.17107322812080383,
0.08187726885080338,
0.0488799624145031,
0.0268076304346323,
-0.030617445707321167,
0.007866011932492256,
0.03032619319856167,
0.046207841485738754,
-0.06210082024335861,
0.04703035578131676,
-0.04916051775217056,
0.04127166420221329,
-0.002781515708193183,
0.0023890018928796053,
-0.04474771022796631,
-0.02449343539774418,
0.03981683775782585,
0.006114952266216278,
-0.0004928084090352058,
0.01504798699170351,
0.013600305654108524,
-0.028502482920885086,
-0.0532689206302166,
0.03398578613996506,
-0.04903494939208031,
-0.03991203382611275,
-0.025317411869764328,
0.015978259965777397,
-0.02413201332092285,
0.0031160442158579826,
0.012945841066539288,
-0.022905902937054634,
-0.04327784478664398,
0.001835268340073526,
0.025541873648762703,
-0.007218300364911556,
0.0569927915930748,
0.35294488072395325,
-0.017474116757512093,
0.05202421918511391,
-0.10320450365543365,
-0.001763652078807354,
-0.029178213328123093,
0.0007148979930207133,
0.03744915872812271,
-0.005528933834284544,
0.04351626709103584,
-0.007746791001409292,
0.041601743549108505,
0.049059562385082245,
0.06128217279911041,
-0.05483530834317207,
0.03825858235359192,
0.03111610747873783,
0.06406945735216141,
-0.06771855056285858,
-0.061707496643066406,
0.06508509069681168,
0.006417698692530394,
-0.06150054559111595,
0.05227740481495857,
0.015155119821429253,
0.04541300609707832,
0.0268472358584404,
-0.013976966962218285,
0.0669621154665947,
0.012213350273668766,
0.031398363411426544,
0.023418277502059937,
0.04737374186515808,
0.03192370757460594,
-0.011569960042834282,
-0.026324884966015816,
-0.013400444760918617,
0.0023762614000588655,
-0.06545014679431915,
0.02920425310730934,
0.029117217287421227,
-0.03063930571079254,
-0.0651765912771225,
-0.06861774623394012,
-0.036664433777332306,
-0.007608575280755758,
0.037607379257678986,
0.026206033304333687,
0.022508999332785606,
-0.04581037163734436,
0.011553156189620495,
0.0744343101978302,
0.05662275478243828,
0.004927397705614567,
0.028057057410478592,
-0.039751019328832626,
0.003933656495064497,
0.0075403843075037,
-0.06179222837090492,
-0.01744757406413555,
0.007166980765759945,
-0.05418112501502037,
-0.060611698776483536,
-0.029964249581098557,
0.1408102959394455,
-0.00882748793810606,
-0.014505188912153244,
-0.046010155230760574,
0.035429082810878754,
0.04357195645570755,
0.03240812197327614,
0.03202764689922333,
-0.026336494833230972,
-0.01990034058690071,
-0.014924928545951843,
-0.0635647177696228,
0.0248617734760046,
0.016593122854828835,
0.017944062128663063,
0.016690751537680626,
0.03308146074414253,
-0.016046205535531044,
-0.021225642412900925,
-0.021117346361279488,
0.07370619475841522,
0.028483327478170395,
0.024857982993125916,
-0.05502616614103317,
0.047331154346466064,
0.017616620287299156,
0.041717469692230225,
-0.041970402002334595,
0.03181679546833038,
-0.04504941776394844,
0.04797697439789772,
-0.06143570318818092,
-0.05238169804215431,
-0.004670299589633942,
-0.010069452226161957,
0.02600960247218609,
-0.053761035203933716,
0.06372945755720139,
0.027821963652968407,
0.011464018374681473,
0.0933675691485405,
0.0026545985601842403,
-0.01637944206595421,
-0.0017725550569593906,
-0.018271086737513542,
0.07144530862569809,
0.011869085021317005,
-0.008269349113106728,
-0.007308963220566511,
-0.008323132060468197,
0.006458261050283909,
-0.033029764890670776,
-0.010652379132807255,
-0.03853248059749603,
-0.01331026665866375,
0.06757982075214386,
-0.036485280841588974,
0.014399607665836811,
-0.05986752361059189,
-0.03420451283454895,
-0.2891658842563629,
-0.032110538333654404,
0.04270893707871437,
-0.051842909306287766,
0.028745416551828384,
-0.03900638595223427,
-0.0020674788393080235,
-0.00018734177865553647,
0.016418419778347015,
-0.08039620518684387,
0.023888548836112022,
0.07326287776231766,
-0.0007944809040054679,
-0.07504367083311081,
-0.0355241484940052,
0.05591445416212082,
0.027475379407405853,
-0.032631922513246536,
0.0257282555103302,
0.040351808071136475,
0.022053278982639313,
-0.041826579719781876,
-0.034272193908691406,
-0.044204648584127426,
0.0446675680577755,
-0.04511420056223869,
0.10914965718984604,
0.0017405225662514567,
0.0008145415340550244,
0.02800666354596615,
0.03979095444083214,
0.025914806872606277,
-0.05196795240044594,
-0.12013590335845947,
-0.004729898180812597,
-0.010994913056492805,
-0.04476969316601753,
0.02543710358440876,
-0.0435042642056942,
-0.02719484269618988,
-0.04378228634595871,
0.004630204755812883,
-0.04529808089137077,
-0.0855807512998581,
-0.024970613420009613,
-0.03626877814531326,
-0.05383169278502464,
0.04939962923526764,
0.021610412746667862,
-0.0167258158326149,
0.02769303135573864,
0.024819791316986084,
-0.029016563668847084,
-0.03133203834295273,
0.012852977029979229,
-0.008748436346650124,
-0.04216806963086128,
0.05336052551865578,
-0.026003070175647736,
-0.019666986539959908,
0.039113372564315796,
0.01718074642121792,
0.02500462718307972,
0.013890380971133709,
0.06530395150184631,
0.006714148446917534,
0.024842092767357826,
-0.058928925544023514,
0.06566950678825378,
0.01247728243470192,
0.030463678762316704,
0.17401769757270813,
-0.01123634073883295,
-0.003268082858994603,
0.02618904784321785,
0.003669656114652753,
-0.07519810646772385,
-0.09575396031141281,
-0.042987190186977386,
0.029419895261526108,
0.025373606011271477,
0.01279880665242672,
0.038786906749010086,
-0.017254892736673355,
-0.011656627990305424,
-0.020392699167132378,
0.029519977048039436,
-0.0009470663499087095,
0.03423261642456055,
0.027513116598129272,
0.027842402458190918,
-0.011331766843795776,
-0.026644829660654068,
-0.04350420832633972,
0.05730494484305382,
0.03279422968626022,
-0.28454384207725525,
-0.005726324860006571,
-0.011604196391999722,
0.05141954869031906,
0.014224653132259846,
-0.009271733462810516,
0.0319199338555336,
-0.02456810511648655,
-0.03965485841035843,
0.03789808601140976,
0.02871936745941639,
0.017400432378053665,
0.03492074832320213,
0.0023727985098958015,
-0.008175890892744064,
0.035320330411195755,
0.03611057996749878,
-0.03985389694571495,
-0.0024051114451140165,
0.007236021105200052,
-0.012509237043559551,
-0.03716409206390381,
0.11964445561170578,
0.039432186633348465,
-0.01841423287987709,
0.059489522129297256,
-0.06015739589929581,
0.0666920393705368,
-0.012585700489580631,
0.01100383885204792,
-0.028141796588897705,
0.05485522747039795,
0.02529209852218628,
-0.03742966800928116,
0.035367224365472794,
0.07195428758859634,
-0.010457199066877365,
-0.016247550025582314,
-0.05935416370630264,
-0.012056158855557442,
-0.05281107500195503,
0.006523010786622763,
-0.0469510592520237,
-0.033925820142030716,
0.05169319733977318,
-0.027101881802082062,
-0.014304876327514648,
-0.03611971437931061,
0.07876675575971603,
-0.04894257336854935,
-0.028356794267892838,
-0.03554559499025345,
-0.0068839844316244125,
0.008610162883996964,
-0.0030637311283499002,
0.01991797238588333,
0.01976822130382061,
-0.05202794075012207,
0.05501466244459152,
0.03076687641441822,
-0.00498095341026783,
-0.056443195790052414,
-0.046940844506025314,
0.09971964359283447,
0.011433822102844715
] |
PMID:25062 | Studies of rat alkaline phosphatase I. Development of methods for detecting isoenzymes. | A manual system of various estimations of rat plasma alkaline phosphatase activity has been devised for small volumes of plasma which uses different inhibitors, compares the utilisation of two substrates and includes acrylamide gel electrophoresis. The different inhibitors etc. allow a degree of discrimination between alkaline phosphatase extracts of rat organs. The properties of isoenzymes, e.g. intestinal phosphatase, differ depending upon the environment in which they are studied. In conjunction, if necessary, with the methods described for the estimation of liver and intestinal alkaline phosphatase activity, it is hoped to use the system to discriminate between the isoenzymes present in the plasma alkaline phosphatase of rats in toxicological studies. | Studies of rat alkaline phosphatase I. Development of methods for detecting isoenzymes. A manual system of various estimations of rat plasma alkaline phosphatase activity has been devised for small volumes of plasma which uses different inhibitors, compares the utilisation of two substrates and includes acrylamide gel electrophoresis. The different inhibitors etc. allow a degree of discrimination between alkaline phosphatase extracts of rat organs. The properties of isoenzymes, e.g. intestinal phosphatase, differ depending upon the environment in which they are studied. In conjunction, if necessary, with the methods described for the estimation of liver and intestinal alkaline phosphatase activity, it is hoped to use the system to discriminate between the isoenzymes present in the plasma alkaline phosphatase of rats in toxicological studies. | [
-0.018492644652724266,
0.00951472483575344,
-0.0033658042084425688,
0.013886387459933758,
0.011637854389846325,
-0.06773652136325836,
0.11380506306886673,
0.06042967364192009,
-0.038885291665792465,
-0.04323079064488411,
0.01891099102795124,
-0.04786060377955437,
-0.03776375949382782,
0.03486147150397301,
-0.017510969191789627,
-0.046347975730895996,
0.04225492849946022,
-0.018014775589108467,
-0.05077420175075531,
-0.015760546550154686,
0.00808101799339056,
0.030863063409924507,
0.07660781592130661,
-0.014661389403045177,
0.03530527651309967,
0.044686704874038696,
0.026312218979001045,
0.006151644978672266,
-0.06996310502290726,
-0.14612096548080444,
-0.010079147294163704,
-0.01025388389825821,
0.06945841759443283,
-0.06819414347410202,
0.0022712862119078636,
0.004289769101887941,
-0.008552093990147114,
0.06387952715158463,
-0.03824267536401749,
0.022083912044763565,
0.03689239174127579,
-0.018675029277801514,
0.013685813173651695,
0.04345697537064552,
0.022695837542414665,
-0.00467008026316762,
-0.023708807304501534,
-0.0038232787046581507,
0.03722973167896271,
0.003564157523214817,
-0.015475495718419552,
0.05951451137661934,
0.04797351360321045,
0.03749435395002365,
0.029024776071310043,
0.022565947845578194,
0.03753513842821121,
0.0776890218257904,
0.00669354060664773,
0.017215853556990623,
0.020786456763744354,
-0.047745443880558014,
-0.13937443494796753,
0.04666779562830925,
0.018458962440490723,
0.03682536259293556,
-0.046030789613723755,
-0.07681199908256531,
0.04992015287280083,
0.010118605569005013,
-0.05103708803653717,
0.07359892129898071,
0.008719570003449917,
-0.018297629430890083,
-0.03234937787055969,
-0.03580523282289505,
0.005936183501034975,
-0.010681060142815113,
-0.009226586669683456,
0.04409566894173622,
-0.038820382207632065,
-0.013592922128736973,
0.06188914179801941,
0.009209701791405678,
-0.0043686083517968655,
-0.0017870805459097028,
0.025995144620537758,
-0.007845068350434303,
0.04844822734594345,
-0.00984952226281166,
-0.04064447432756424,
-0.05630004405975342,
-0.02376588247716427,
-0.019518040120601654,
-0.023900436237454414,
0.032754864543676376,
0.0491982027888298,
0.0012763289269059896,
0.03953725844621658,
0.36085590720176697,
-0.051623471081256866,
-0.004410381428897381,
-0.09904768317937851,
-0.062036558985710144,
-0.041749291121959686,
-0.015850504860281944,
-0.07960866391658783,
-0.035268597304821014,
-0.07051469385623932,
0.0529678612947464,
0.07545729726552963,
0.03734845668077469,
-0.020106440410017967,
-0.042908623814582825,
-0.05063512921333313,
-0.036860257387161255,
0.010549230501055717,
0.04059404134750366,
0.01820443384349346,
0.045284952968358994,
0.014921918511390686,
-0.02663026563823223,
0.005503744352608919,
-0.06274683028459549,
0.04176986962556839,
0.025660015642642975,
0.04105056822299957,
0.08299758285284042,
0.05362197756767273,
0.026967784389853477,
0.038552068173885345,
0.024026643484830856,
-0.017536316066980362,
0.006082736887037754,
-0.0037996608298271894,
-0.00602847570553422,
0.04538694769144058,
0.01566178724169731,
0.07425466179847717,
0.005485777743160725,
-0.047141462564468384,
-0.053823716938495636,
-0.025053225457668304,
-0.04187679663300514,
-0.0413665696978569,
0.11179118603467941,
-0.03368033096194267,
0.01833951473236084,
-0.027694839984178543,
-0.12413113564252853,
-0.0017064575804397464,
0.02246714197099209,
-0.005739438347518444,
0.03927525132894516,
-0.0566125363111496,
0.004013307858258486,
-0.004827858880162239,
-0.021435104310512543,
-0.004598458297550678,
0.004357554018497467,
-0.10319669544696808,
-0.03205300122499466,
-0.00330295879393816,
0.058555103838443756,
0.04247916862368584,
0.03930089622735977,
-0.008528478443622589,
0.0058442410081624985,
-0.0036118158604949713,
-0.04045705497264862,
-0.007202930748462677,
-0.002205359283834696,
-0.012387438677251339,
-0.017378799617290497,
-0.11187196522951126,
-0.031201867386698723,
0.053973350673913956,
0.04468201473355293,
-0.012124830856919289,
-0.0393373966217041,
0.0006623464869335294,
-0.04948800057172775,
-0.01607595756649971,
0.0741409957408905,
-0.005793693009763956,
-0.027828413993120193,
-0.018723314628005028,
0.007170212920755148,
-0.012349649332463741,
0.10861783474683762,
0.023323601111769676,
0.025116749107837677,
-0.053601231426000595,
-0.008680960163474083,
-0.04444481059908867,
-0.024432692676782608,
-0.029704906046390533,
-0.0019208267331123352,
0.00878399983048439,
-0.04096851497888565,
0.07844744622707367,
0.02972412295639515,
0.040003448724746704,
0.017787957563996315,
-0.005167478695511818,
0.09341038763523102,
-0.036611638963222504,
0.019404515624046326,
0.11570195108652115,
0.035623736679553986,
-0.047109127044677734,
0.00002679079261724837,
-0.053258493542671204,
0.016452038660645485,
-0.03290854021906853,
0.021512208506464958,
0.0004868157848250121,
-0.023616936057806015,
0.066627636551857,
0.025028927251696587,
0.002235939959064126,
-0.03380037471652031,
-0.02942240983247757,
-0.2955448031425476,
-0.03872230276465416,
-0.003286548424512148,
0.013425865210592747,
0.04650535061955452,
-0.02162417769432068,
0.010340044274926186,
-0.04118580371141434,
-0.03114999085664749,
-0.027561502531170845,
0.04792168736457825,
0.029713764786720276,
0.029868433251976967,
0.044365573674440384,
0.012501135468482971,
0.056620970368385315,
-0.03482982516288757,
-0.0299022626131773,
0.03505890816450119,
-0.029808182269334793,
0.02256554551422596,
-0.0246298685669899,
0.062285032123327255,
0.028862064704298973,
0.03272268548607826,
0.014701752923429012,
0.11025556921958923,
0.0006301123648881912,
-0.018695179373025894,
-0.05482320860028267,
0.007356525864452124,
0.045079804956912994,
0.03959750384092331,
-0.08655829727649689,
-0.021276943385601044,
-0.04619579389691353,
-0.0701812356710434,
-0.033966511487960815,
-0.025012625381350517,
0.01775665581226349,
-0.054013557732105255,
-0.007819386199116707,
-0.013364878483116627,
-0.04852594435214996,
-0.044749077409505844,
0.003974058665335178,
0.04210005700588226,
0.007484036963433027,
0.02320864424109459,
-0.03457099199295044,
0.010619289241731167,
0.0018497740384191275,
0.0065341186709702015,
-0.055363018065690994,
-0.035510942339897156,
-0.0002971958601847291,
-0.025832796469330788,
0.005192779004573822,
-0.06416718661785126,
0.007749902084469795,
0.010602853260934353,
-0.03038530796766281,
-0.003006602171808481,
-0.019229860976338387,
0.016446733847260475,
-0.004590129945427179,
-0.036397095769643784,
-0.010028013959527016,
0.017368050292134285,
0.02638494037091732,
0.03183392062783241,
0.11057313531637192,
-0.018874159082770348,
0.01502733863890171,
0.047339316457509995,
0.0023242265451699495,
-0.034359727054834366,
-0.012995650060474873,
-0.038762230426073074,
0.03270841762423515,
-0.009603798389434814,
-0.07749006152153015,
0.07571776956319809,
-0.0007941226940602064,
0.00794356968253851,
0.0747305229306221,
0.03504658117890358,
-0.018308032304048538,
0.06952333450317383,
0.022320767864584923,
0.04475006088614464,
0.028868477791547775,
-0.05188503488898277,
-0.020162058994174004,
0.05692528188228607,
0.037761226296424866,
-0.25956663489341736,
0.031770385801792145,
-0.0163840651512146,
-0.04148462414741516,
0.013794026337563992,
-0.012504849582910538,
0.020304599776864052,
-0.016500474885106087,
-0.004730313550680876,
0.03294166550040245,
0.061030514538288116,
0.03759203106164932,
0.06662940979003906,
0.011227628216147423,
-0.04639992117881775,
0.01524430513381958,
0.03849155455827713,
0.002186802914366126,
0.029940061271190643,
-0.045169368386268616,
0.04623870924115181,
-0.08397264778614044,
0.17079661786556244,
-0.012582624331116676,
-0.00998836848884821,
0.08032363653182983,
-0.0033508886117488146,
-0.015253647230565548,
-0.04816269129514694,
-0.02525642327964306,
0.008155707269906998,
0.032069217413663864,
0.0402856208384037,
-0.03535079210996628,
-0.006009608041495085,
0.07960069179534912,
0.007305113598704338,
0.012139818631112576,
-0.03485485166311264,
-0.012957138940691948,
0.0036096246913075447,
-0.026606623083353043,
-0.12497879564762115,
-0.0459420420229435,
0.048322342336177826,
-0.05523145943880081,
-0.044315848499536514,
-0.03638971969485283,
0.01589355617761612,
-0.006908133160322905,
-0.055176325142383575,
-0.042317021638154984,
-0.0026851205620914698,
0.05454767122864723,
0.040009405463933945,
-0.016515186056494713,
-0.06037761643528938,
-0.034790944308042526,
0.004115666262805462,
-0.037030886858701706,
0.07537861168384552,
0.020587915554642677,
-0.011028881184756756,
0.09451904147863388,
0.015972286462783813
] |
PMID:25057 | [Experimental studies of the specificity and aspecificity of a beta-adrenolytic agent, bunitrolol (Kö 1366)]. | In basis of our experimental researches bunitrolol presented: a) beta-adrenolytic activity prevailingly at cardiac level (superior to propranolol and practolol) in respect to vascular, tracheal, bronchial, uterus, intestinal and metabolic level; b) beta-adrenergic intrinsic activity; c) local anesthetic activity; d) chinidino-like activity; e) antiarrhythmic activity; f) hypotensive activity with interferences at the level of vasomotor centres, of the barosensitive zones, of the renin secretion and of the purinergic periphery. | [Experimental studies of the specificity and aspecificity of a beta-adrenolytic agent, bunitrolol (Kö 1366)]. In basis of our experimental researches bunitrolol presented: a) beta-adrenolytic activity prevailingly at cardiac level (superior to propranolol and practolol) in respect to vascular, tracheal, bronchial, uterus, intestinal and metabolic level; b) beta-adrenergic intrinsic activity; c) local anesthetic activity; d) chinidino-like activity; e) antiarrhythmic activity; f) hypotensive activity with interferences at the level of vasomotor centres, of the barosensitive zones, of the renin secretion and of the purinergic periphery. | [
-0.039856813848018646,
-0.005543960258364677,
0.013927695341408253,
0.025612499564886093,
0.017525816336274147,
-0.03960200026631355,
0.03781994432210922,
0.044873908162117004,
-0.02180219627916813,
-0.04835992678999901,
-0.0017816078616306186,
-0.03159291669726372,
-0.019359881058335304,
0.05148094892501831,
-0.01819302886724472,
-0.025750847533345222,
0.05343545228242874,
-0.06096835806965828,
-0.09153017401695251,
-0.009296520613133907,
-0.011795904487371445,
-0.010585267096757889,
0.039664968848228455,
0.02553722821176052,
-0.005080852657556534,
-0.0038187867030501366,
0.056289397180080414,
-0.02781190536916256,
-0.03858425095677376,
-0.1447310447692871,
-0.004843840841203928,
-0.008563194423913956,
0.07515077292919159,
-0.010301859118044376,
-0.021576309576630592,
0.009544267319142818,
-0.02962890826165676,
0.027425652369856834,
-0.07212718576192856,
0.03344213217496872,
0.08766491711139679,
-0.020409313961863518,
0.00867151003330946,
0.04235314577817917,
0.044717419892549515,
-0.00966295599937439,
-0.026751091703772545,
-0.023020120337605476,
0.04209441319108009,
0.026068899780511856,
0.04492887109518051,
0.006446439772844315,
-0.007360855117440224,
0.02465461939573288,
0.05028878152370453,
-0.07412952184677124,
-0.0063833212479949,
0.02795885130763054,
-0.01324751228094101,
0.021367747336626053,
-0.012837950140237808,
0.04213450849056244,
-0.1506262719631195,
0.024381661787629128,
-0.02936996892094612,
0.05622120574116707,
-0.025072982534766197,
-0.03959216922521591,
0.023639682680368423,
0.05212651938199997,
0.028412170708179474,
0.05605118349194527,
0.0010952828451991081,
0.010992484167218208,
-0.007839903235435486,
-0.011624712496995926,
0.0015035298420116305,
-0.0015952741960063577,
-0.0414932556450367,
-0.002266481053084135,
0.03997496888041496,
0.04752044379711151,
0.010519260540604591,
-0.009695515036582947,
0.02936864085495472,
-0.015321384184062481,
0.06073649227619171,
-0.03251977637410164,
0.016869740560650826,
-0.03649822250008583,
-0.06972295790910721,
-0.0009332237532362342,
-0.03602980077266693,
-0.03459246829152107,
-0.017091330140829086,
0.021209046244621277,
-0.09026288241147995,
-0.0038679002318531275,
-0.024064723402261734,
0.36553093791007996,
-0.03502292558550835,
0.04567612707614899,
-0.10052616149187088,
-0.007066868245601654,
0.03105812892317772,
-0.01188565045595169,
-0.013822274282574654,
0.000020821165890083648,
-0.0032949172891676426,
0.03595463186502457,
0.05156094953417778,
0.016386475414037704,
-0.0042943493463099,
-0.026072731241583824,
-0.026726342737674713,
-0.017825897783041,
0.011583257466554642,
0.015854062512516975,
-0.011085067875683308,
0.022809455171227455,
-0.0013468237593770027,
-0.011656484566628933,
0.015806609764695168,
-0.002087715547531843,
0.06892753392457962,
0.015437308698892593,
-0.04659730941057205,
0.04002666100859642,
0.016277920454740524,
0.025480611249804497,
0.029418935999274254,
0.07140636444091797,
0.010475694201886654,
-0.011677993461489677,
0.0026696391869336367,
-0.030176982283592224,
0.038547415286302567,
-0.006269307341426611,
0.0644242987036705,
-0.042677801102399826,
-0.019825374707579613,
-0.005463211797177792,
0.009057288058102131,
-0.07878623157739639,
-0.07464677840471268,
0.08655577152967453,
0.05426006019115448,
0.017822064459323883,
0.0030050217173993587,
0.0328555554151535,
-0.038345012813806534,
0.0144042344763875,
-0.04308789223432541,
0.04912383109331131,
-0.006384716369211674,
-0.009990469552576542,
0.0029661082662642,
0.07299007475376129,
-0.02801482565701008,
-0.07614302635192871,
-0.11417699605226517,
-0.06506945937871933,
-0.018921930342912674,
0.06380326300859451,
0.020175857469439507,
0.004040269646793604,
-0.023715367540717125,
0.028674768283963203,
0.010612604208290577,
-0.014272801578044891,
-0.0072208126075565815,
-0.0148444389924407,
-0.02749883197247982,
0.030031196773052216,
-0.1028529554605484,
0.028446080163121223,
0.029919758439064026,
-0.01222249399870634,
0.03738182410597801,
-0.03844157233834267,
0.021267516538500786,
-0.006035856902599335,
0.030938880518078804,
-0.02982487343251705,
0.013453605584800243,
0.01951412856578827,
-0.02503274194896221,
-0.07688387483358383,
-0.017367683351039886,
0.05691126361489296,
-0.01869232952594757,
-0.028593534603714943,
-0.05882232263684273,
-0.011857830919325352,
-0.02002205327153206,
-0.025767764076590538,
-0.030379878357052803,
0.023247309029102325,
-0.06226737052202225,
-0.010177956894040108,
0.09475327283143997,
-0.010725264437496662,
0.037368714809417725,
0.007294727023690939,
-0.009340056218206882,
0.07434418797492981,
-0.000777884793933481,
-0.018139144405722618,
0.05062389746308327,
-0.0270095132291317,
0.013291073963046074,
0.06551148742437363,
0.013196944259107113,
0.061715174466371536,
-0.07740168273448944,
0.042702943086624146,
-0.06947934627532959,
0.015462489798665047,
0.07361506670713425,
-0.001176613150164485,
0.0015810822369530797,
0.005323826801031828,
0.01552353985607624,
-0.3282569348812103,
-0.0470605194568634,
-0.022296497598290443,
-0.05335696041584015,
0.07451193034648895,
-0.02863612398505211,
-0.016251634806394577,
-0.05562705174088478,
-0.008684528060257435,
-0.07887249439954758,
0.05645444244146347,
0.032478030771017075,
-0.036600079387426376,
0.037927329540252686,
0.01998896338045597,
0.05815742537379265,
-0.0032665252219885588,
-0.0336751751601696,
-0.01515963114798069,
0.006796266883611679,
0.02562600187957287,
-0.0475168339908123,
-0.012438380159437656,
0.03663007169961929,
0.005110881291329861,
-0.007000887766480446,
0.08745954930782318,
-0.02074652537703514,
0.03289073705673218,
-0.000851774646434933,
0.017206421121954918,
0.019511809572577477,
-0.022057535126805305,
-0.10831847786903381,
0.0654589906334877,
-0.04656199365854263,
0.07536035031080246,
-0.027859177440404892,
0.029018402099609375,
-0.015526666305959225,
-0.0200722087174654,
0.047053225338459015,
0.0357617512345314,
-0.049482982605695724,
-0.03618621081113815,
0.015376272611320019,
0.011791802942752838,
0.07460269331932068,
-0.038901809602975845,
0.004639919847249985,
-0.02622740902006626,
-0.007755029946565628,
-0.019802436232566833,
-0.018985815346240997,
0.02180887572467327,
-0.028505412861704826,
-0.10043369233608246,
0.0012175105512142181,
-0.019261453300714493,
0.03172130137681961,
0.041054416447877884,
0.013257218524813652,
0.03246043622493744,
-0.0023416411131620407,
-0.03889911249279976,
0.0384652353823185,
0.00901575293391943,
-0.006601620931178331,
0.047099124640226364,
0.03684619441628456,
-0.01524353213608265,
0.0794178918004036,
-0.03808266296982765,
0.02009858563542366,
0.04204527661204338,
-0.009195784106850624,
0.0028464465867727995,
-0.04481016844511032,
0.011612324975430965,
0.047952741384506226,
-0.023623693734407425,
-0.07580271363258362,
0.02958136983215809,
-0.052596565335989,
-0.006284620612859726,
-0.013326896354556084,
-0.0010650972835719585,
-0.013877520337700844,
0.08864705264568329,
-0.022928956896066666,
-0.0326717384159565,
0.01888447254896164,
-0.05238105729222298,
-0.0411161407828331,
0.07853243499994278,
0.043844278901815414,
-0.26808926463127136,
-0.011802932247519493,
0.060485031455755234,
-0.016569538041949272,
0.007646764628589153,
-0.044460639357566833,
0.01768410950899124,
-0.04373406991362572,
-0.011876258999109268,
-0.008413871750235558,
0.012501897290349007,
0.010412600822746754,
0.06427746266126633,
0.05136650800704956,
-0.05010348930954933,
0.000880257342942059,
0.05133379250764847,
0.016138937324285507,
0.02046545222401619,
-0.008171302266418934,
-0.007588823791593313,
0.008091031573712826,
0.1414564847946167,
0.043567802757024765,
-0.07158004492521286,
0.08104360103607178,
0.027329424396157265,
0.039024718105793,
-0.08058514446020126,
0.038713596761226654,
-0.0028957377653568983,
-0.01867862418293953,
0.08219915628433228,
-0.03826505318284035,
0.0247831791639328,
0.0780661404132843,
0.016528625041246414,
0.04314356669783592,
-0.024506522342562675,
-0.03409178927540779,
-0.032795440405607224,
0.006433415692299604,
-0.06936296075582504,
-0.057413917034864426,
0.07825691252946854,
-0.11218728125095367,
-0.05153079703450203,
-0.009209716692566872,
0.01526174321770668,
0.0538257472217083,
-0.018342262133955956,
-0.04133688658475876,
0.047449011355638504,
0.06685907393693924,
0.09162769466638565,
-0.024893978610634804,
-0.006427529267966747,
-0.08229212462902069,
0.0210825614631176,
0.00982822384685278,
-0.0346926748752594,
0.015190946869552135,
-0.017298173159360886,
0.06829274445772171,
0.0663374811410904
] |
PMID:25058 | [Experimental analysis of the cardiovascular effects of acebutolol, a drug with beta-adrenolytic activity]. | In experiments at cardiovascular system level, acebutolol was found to possess beta-adrenolytic activity of cardioselective type associated with intrinsic beta-adrenergic, antiarrhythmic and anti-hypertensive activity. It was well tolerated systemically. | [Experimental analysis of the cardiovascular effects of acebutolol, a drug with beta-adrenolytic activity]. In experiments at cardiovascular system level, acebutolol was found to possess beta-adrenolytic activity of cardioselective type associated with intrinsic beta-adrenergic, antiarrhythmic and anti-hypertensive activity. It was well tolerated systemically. | [
-0.05016511306166649,
0.006431639194488525,
-0.025737391784787178,
0.03945523872971535,
0.016553038731217384,
-0.03544222563505173,
0.028051206842064857,
-0.014809862710535526,
-0.04983282834291458,
-0.07654687762260437,
-0.022751200944185257,
-0.017809655517339706,
0.01040758565068245,
0.07002995163202286,
-0.032916732132434845,
-0.0019271571654826403,
0.059116002172231674,
-0.002197869587689638,
-0.03902144730091095,
0.014797019772231579,
-0.009310570545494556,
0.044272907078266144,
-0.041515544056892395,
0.02946232073009014,
0.03930523991584778,
0.047887708991765976,
0.04067489504814148,
0.057041265070438385,
-0.07066599279642105,
-0.11841649562120438,
-0.011127101257443428,
0.027677930891513824,
0.05569924786686897,
-0.019543839618563652,
0.016933083534240723,
0.019360890612006187,
0.0072888643480837345,
0.0751318708062172,
-0.09341151267290115,
0.0989157184958458,
0.022115318104624748,
-0.031112585216760635,
-0.03387940675020218,
0.052699703723192215,
0.004847817122936249,
-0.04272400587797165,
-0.042362798005342484,
-0.008131422102451324,
0.0717070996761322,
0.02970104292035103,
0.04660592973232269,
-0.014820841141045094,
-0.0010200993856415153,
0.01921175606548786,
0.03633744642138481,
-0.07768510282039642,
-0.038738079369068146,
0.051041655242443085,
0.03006337396800518,
0.007278131786733866,
0.01248963549733162,
0.02268364280462265,
-0.13105392456054688,
0.05833949148654938,
-0.017284385859966278,
0.08153589814901352,
-0.07683934271335602,
0.046918656677007675,
0.03838817775249481,
0.038308240473270416,
-0.006116543430835009,
0.004797872621566057,
0.017664549872279167,
0.012377926148474216,
-0.024102061986923218,
-0.01333499513566494,
0.012473849579691887,
-0.012482920661568642,
-0.03418122977018356,
-0.015262440778315067,
0.041241034865379333,
-0.06830979883670807,
0.017571350559592247,
0.003699567634612322,
0.020807258784770966,
-0.04010244831442833,
0.09306230396032333,
-0.02275834232568741,
-0.030945101752877235,
-0.03536219894886017,
-0.041217412799596786,
-0.01456381380558014,
0.0014955714577808976,
0.04508999362587929,
0.021045252680778503,
0.005741898901760578,
-0.05771800875663757,
-0.06749224662780762,
0.006682849023491144,
0.35273849964141846,
-0.02871791459619999,
0.04711345583200455,
-0.07053698599338531,
-0.03107273392379284,
0.024325639009475708,
-0.009909583255648613,
-0.04026433080434799,
-0.0005353427841328084,
-0.02705470658838749,
0.029121313244104385,
0.04535967856645584,
-0.038566622883081436,
0.027850696817040443,
-0.016962707042694092,
-0.01868610270321369,
-0.030893974006175995,
0.022930152714252472,
-0.014192430302500725,
-0.0171448215842247,
0.02206180989742279,
-0.028514161705970764,
-0.03966492414474487,
0.04040326178073883,
0.017134640365839005,
0.040110133588314056,
-0.01470265444368124,
0.0022735532838851213,
0.07425545156002045,
0.08466792106628418,
-0.01082365307956934,
0.05721097066998482,
0.0066382200457155704,
-0.044700656086206436,
-0.014027220197021961,
0.016090890392661095,
-0.01963936910033226,
0.029148947447538376,
0.03073018789291382,
0.02346576564013958,
-0.0031295246444642544,
0.023625854402780533,
0.027177173644304276,
0.06775931268930435,
-0.09475652873516083,
-0.07503901422023773,
0.08272559195756912,
0.07851647585630417,
0.02668691799044609,
-0.023244868963956833,
0.052748434245586395,
-0.0513157844543457,
-0.018074698746204376,
-0.01983189769089222,
0.10389108955860138,
-0.017720453441143036,
0.01013725996017456,
0.06080208718776703,
0.06822849065065384,
0.00046450868831016123,
-0.022718673571944237,
-0.1146463006734848,
-0.06086798384785652,
-0.043707456439733505,
0.036471135914325714,
-0.012518569827079773,
0.010647050105035305,
-0.04455270618200302,
0.017066510394215584,
-0.041558291763067245,
0.02294289879500866,
-0.023200353607535362,
-0.04327163100242615,
-0.010033017955720425,
-0.029822416603565216,
-0.0748334750533104,
0.05607447773218155,
0.04663122445344925,
-0.014702859334647655,
-0.016323335468769073,
-0.016294263303279877,
0.05130167305469513,
0.004976940341293812,
0.0008681447361595929,
-0.008834760636091232,
0.023391682654619217,
0.00450148293748498,
-0.05839719623327255,
-0.035554371774196625,
0.03081498108804226,
0.03693138435482979,
-0.0016402110923081636,
-0.00531026441603899,
-0.03843889385461807,
-0.02921837754547596,
0.0053892964497208595,
-0.052762266248464584,
-0.013029954396188259,
0.00662857573479414,
-0.038401659578084946,
0.03520262986421585,
0.0947815328836441,
0.03481564298272133,
-0.0026093509513884783,
0.01744871400296688,
0.0362451933324337,
0.08474761992692947,
-0.0513615719974041,
-0.028608685359358788,
0.04296030476689339,
-0.029757695272564888,
0.0168350450694561,
-0.006047078873962164,
-0.003787412540987134,
0.05583595111966133,
-0.07079433649778366,
0.0045386189594864845,
-0.05221813544631004,
-0.008635365404188633,
0.041655171662569046,
-0.02858271636068821,
0.016658252105116844,
-0.019208241254091263,
0.049398962408304214,
-0.32197055220603943,
-0.05934807285666466,
-0.05482940003275871,
-0.04565630853176117,
0.02166779525578022,
-0.03577776253223419,
0.03899296000599861,
-0.0846426710486412,
-0.011981639079749584,
-0.06387558579444885,
0.02114156074821949,
0.0013811247190460563,
0.04209984466433525,
-0.01428183913230896,
-0.012712959200143814,
0.059035707265138626,
0.0026636975817382336,
-0.08413394540548325,
-0.011564986780285835,
0.05093144625425339,
0.00965707004070282,
-0.03605389967560768,
-0.020024416968226433,
0.03164295479655266,
0.08364767581224442,
-0.015040656551718712,
0.06870553642511368,
-0.01596040464937687,
-0.023353004828095436,
0.01249551773071289,
0.009694247506558895,
0.03150234743952751,
0.05665741115808487,
-0.12065411359071732,
-0.024426454678177834,
-0.021612832322716713,
0.05648449435830116,
-0.025371918454766273,
0.008463580161333084,
0.024135293439030647,
-0.0882234126329422,
-0.02352023310959339,
0.018773863092064857,
-0.041439421474933624,
-0.07596388459205627,
0.01930546946823597,
0.05811738595366478,
0.03870150074362755,
-0.09059703350067139,
0.05930962413549423,
-0.0518164187669754,
0.0066847242414951324,
0.03835129737854004,
0.023679664358496666,
0.023196471855044365,
0.0019554272294044495,
-0.07880952209234238,
0.028653401881456375,
-0.008033745922148228,
-0.01723211444914341,
0.036626994609832764,
0.0066777002066373825,
-0.01983235962688923,
0.06802885234355927,
-0.0021153930574655533,
0.013407022692263126,
0.027233464643359184,
-0.011554230004549026,
0.03335133567452431,
0.002554854843765497,
0.006096299737691879,
0.09775081276893616,
-0.014861635863780975,
-0.03084874339401722,
0.07550755888223648,
0.017546987161040306,
0.022569376975297928,
-0.04783786088228226,
-0.08006570488214493,
-0.0069540124386549,
-0.06840250641107559,
-0.07644878327846527,
0.0425737127661705,
-0.005411271471530199,
-0.021356096491217613,
-0.01838049106299877,
0.00793458055704832,
0.011985675431787968,
0.023015473037958145,
-0.06360745429992676,
-0.04901335760951042,
-0.04927021637558937,
-0.00698331743478775,
-0.005882877390831709,
0.032186511904001236,
-0.025359220802783966,
-0.2525445520877838,
-0.02591775357723236,
0.07451330125331879,
-0.02040250599384308,
-0.001625221804715693,
-0.03786249831318855,
0.03605953976511955,
0.004478917457163334,
-0.05788860470056534,
-0.004393462557345629,
-0.0036313424352556467,
0.022774310782551765,
0.061129335314035416,
0.03614261373877525,
-0.06648790091276169,
0.03324541449546814,
0.046309374272823334,
-0.00016487116226926446,
0.03860784322023392,
0.002502262592315674,
0.01644056662917137,
0.02395722083747387,
0.11462163180112839,
0.018183868378400803,
-0.043597038835287094,
0.09170863777399063,
-0.02428852766752243,
-0.004069317132234573,
-0.05457088723778725,
0.047790613025426865,
0.0008128420449793339,
-0.02042684704065323,
0.0701984167098999,
-0.016339758411049843,
0.038892246782779694,
0.04268436133861542,
-0.03909553214907646,
-0.00027891635545529425,
0.02788735367357731,
0.0017657753778621554,
-0.01099420990794897,
-0.012408273294568062,
-0.08946633338928223,
-0.0028488507959991693,
0.06937985122203827,
-0.018188968300819397,
-0.07520955055952072,
0.018702108412981033,
0.04365687817335129,
0.0512356162071228,
-0.034161124378442764,
-0.033095523715019226,
0.019419677555561066,
0.06943615525960922,
0.046004146337509155,
-0.030330389738082886,
-0.046479858458042145,
-0.03885304555296898,
0.03994112089276314,
0.024139996618032455,
0.0362628810107708,
-0.008782781660556793,
-0.023265011608600616,
0.07689261436462402,
0.05280220881104469
] |
PMID:25063 | Local injuries by accidental ingestion of corrosive substances by children. | Own data and analysis of previous publications show that situations where accidental ingestion of corrosive substances by children may have happened are frequent, but severe corrosive esophagitis leading to perforation or stricture formation is very rare. In case of suspected esophageal injury, esophagoscopy and glucocorticoid treatment become necessary. The evaluation of the initial symptoms in patients from our own material and from the literature indicates that all children with serious esophageal burns had one or more of the following symptoms: visible burns in the oral cavity, hypersalivation, retching, vomiting, retrosternal or epigastric pain, cardiovascular collaps, airway stenosis. Hence, children with an uncertain history of ingestion and without any of these symptoms need not be treated. After ingestion of liquid substances, but never of dry or granular products, lesions in the esophagus without accompanying burns in the oral cavity were observed. The evaluation of 1158 cases of accidental ingestions of several types of household products and a collection of data from the literature on the causticity of these substances shows that cleaners containing mainly detergents and phosphates (with pH values generally between 9 and 11), and household bleaches on sodium hypochlorite basis, are relatively harmless. Drain cleaners (NaOH), decalcifiers (formic acid) and detergents for automatic dish washing machines (metasilicates) are very caustic and are responsible for the majority of serious accidents in children. | Local injuries by accidental ingestion of corrosive substances by children. Own data and analysis of previous publications show that situations where accidental ingestion of corrosive substances by children may have happened are frequent, but severe corrosive esophagitis leading to perforation or stricture formation is very rare. In case of suspected esophageal injury, esophagoscopy and glucocorticoid treatment become necessary. The evaluation of the initial symptoms in patients from our own material and from the literature indicates that all children with serious esophageal burns had one or more of the following symptoms: visible burns in the oral cavity, hypersalivation, retching, vomiting, retrosternal or epigastric pain, cardiovascular collaps, airway stenosis. Hence, children with an uncertain history of ingestion and without any of these symptoms need not be treated. After ingestion of liquid substances, but never of dry or granular products, lesions in the esophagus without accompanying burns in the oral cavity were observed. The evaluation of 1158 cases of accidental ingestions of several types of household products and a collection of data from the literature on the causticity of these substances shows that cleaners containing mainly detergents and phosphates (with pH values generally between 9 and 11), and household bleaches on sodium hypochlorite basis, are relatively harmless. Drain cleaners (NaOH), decalcifiers (formic acid) and detergents for automatic dish washing machines (metasilicates) are very caustic and are responsible for the majority of serious accidents in children. | [
-0.00657120393589139,
-0.022899186238646507,
0.04470006749033928,
0.020362162962555885,
0.01926698349416256,
-0.0753757432103157,
0.08745961636304855,
0.0394272655248642,
-0.09369383007287979,
-0.05179009214043617,
-0.05576794221997261,
-0.07211614400148392,
-0.010872012935578823,
0.040122006088495255,
-0.01602195017039776,
0.0242619551718235,
-0.002119603333994746,
-0.005873159505426884,
-0.09571677446365356,
-0.050501517951488495,
0.015826020389795303,
0.04746289923787117,
-0.004720874130725861,
-0.06065252423286438,
-0.003969570156186819,
0.02754921279847622,
0.03412259370088577,
0.009310532361268997,
-0.08737657964229584,
-0.1495102494955063,
0.017863180488348007,
-0.036480918526649475,
-0.028857100754976273,
-0.05932805314660072,
-0.037610724568367004,
-0.015575580298900604,
0.023175209760665894,
0.07687044888734818,
0.0038614431396126747,
0.002766100922599435,
0.015322569757699966,
0.024014903232455254,
-0.016138775274157524,
0.0027987416833639145,
-0.03429894894361496,
0.048451293259859085,
-0.011639893986284733,
-0.007645515725016594,
0.14774538576602936,
-0.03176058083772659,
0.0021503448951989412,
-0.03766298294067383,
0.04029393568634987,
0.07320237904787064,
0.002884530695155263,
-0.03796536102890968,
0.004439171403646469,
-0.043221861124038696,
0.049970727413892746,
0.031099341809749603,
-0.00026221637381240726,
0.03311190381646156,
-0.12248014658689499,
0.056497376412153244,
0.011983954347670078,
0.014498489908874035,
-0.0054013715125620365,
-0.022484511137008667,
0.10543683171272278,
0.016686785966157913,
-0.019276190549135208,
0.026858380064368248,
-0.01213874388486147,
0.12316042184829712,
-0.03109816648066044,
0.00007746642950223759,
0.03085637465119362,
-0.07118941098451614,
-0.070881687104702,
0.07413355261087418,
0.014026502147316933,
0.020715028047561646,
-0.006606964860111475,
-0.025159841403365135,
-0.006054850295186043,
-0.07345869392156601,
0.02259323000907898,
-0.06503762304782867,
0.0009968769736588001,
-0.011827949434518814,
-0.010591503232717514,
-0.001455593854188919,
0.014920481480658054,
0.019825343042612076,
-0.002738070674240589,
-0.01905258186161518,
-0.0005577389965765178,
0.027019808068871498,
-0.045275114476680756,
0.35577574372291565,
-0.021701030433177948,
0.007535506505519152,
0.006672766525298357,
-0.04696328192949295,
0.011432125233113766,
-0.007235179655253887,
0.03693827986717224,
-0.03093552403151989,
-0.02005155012011528,
0.010341956280171871,
0.042315803468227386,
0.037209413945674896,
0.028541039675474167,
-0.029820410534739494,
-0.06858068704605103,
-0.013522404246032238,
0.08514361083507538,
-0.006814889144152403,
-0.07647822052240372,
-0.007896911352872849,
0.0047492762096226215,
0.039152029901742935,
0.04957601800560951,
0.014180504716932774,
0.039028238505125046,
-0.005703296046704054,
0.011491525918245316,
0.0647236630320549,
0.03160838410258293,
0.03691054508090019,
0.006684686057269573,
-0.0844200849533081,
0.028919711709022522,
0.008116450160741806,
0.030825702473521233,
0.0023138190153986216,
0.014152761548757553,
-0.004795649088919163,
0.038008857518434525,
-0.01161759253591299,
0.009579643607139587,
-0.09450376778841019,
-0.027395660057663918,
-0.08207447826862335,
0.0003270146844442934,
0.05645911768078804,
-0.027777478098869324,
-0.002061577746644616,
-0.08112569153308868,
-0.03997458145022392,
-0.025262514129281044,
0.03439703211188316,
-0.027173638343811035,
0.07103818655014038,
-0.007591817528009415,
0.005510163027793169,
0.01268589124083519,
-0.02228754200041294,
0.03103969804942608,
-0.02163233608007431,
0.0316397063434124,
-0.03871091082692146,
-0.008090168237686157,
0.015345396474003792,
-0.012084356509149075,
0.016789507120847702,
-0.006713123992085457,
-0.01499961782246828,
0.002806996461004019,
0.009164340794086456,
-0.006006898358464241,
0.038843657821416855,
-0.009218084625899792,
-0.021969804540276527,
-0.03340573236346245,
0.06268249452114105,
0.02059084177017212,
0.017570994794368744,
-0.009997046552598476,
-0.008305221796035767,
0.04759674519300461,
0.0174405574798584,
0.007967418991029263,
0.02709558978676796,
0.023488638922572136,
-0.031402837485075,
0.01976187154650688,
-0.04114707559347153,
0.05221310630440712,
0.0026315590366721153,
-0.030651597306132317,
-0.09456498175859451,
-0.11571740359067917,
-0.035257738083601,
0.006717648822814226,
0.018047237768769264,
-0.038104236125946045,
-0.038305021822452545,
0.03261202946305275,
0.027071869000792503,
0.04582825303077698,
-0.02387363649904728,
0.03197762370109558,
0.022131124511361122,
0.03002621978521347,
-0.004040994215756655,
-0.006599242798984051,
0.011334029957652092,
0.021632535383105278,
-0.03961751610040665,
-0.0011670817621052265,
0.03388220816850662,
-0.026472005993127823,
-0.00593585567548871,
0.022958965972065926,
0.010111188516020775,
-0.010817508213222027,
0.033938419073820114,
-0.020657090470194817,
-0.029855316504836082,
0.04018430411815643,
-0.019442973658442497,
-0.01538797840476036,
-0.33894088864326477,
-0.021921012550592422,
0.0019312668591737747,
-0.008988483808934689,
0.04496743530035019,
0.029510173946619034,
0.06262975186109543,
-0.06626560539007187,
-0.03999112546443939,
-0.0632958859205246,
0.018475521355867386,
0.0539412796497345,
-0.05466761067509651,
0.03519529476761818,
-0.00028971576830372214,
0.0067710066214203835,
0.05259208008646965,
-0.02785751037299633,
0.03216154873371124,
0.03752266243100166,
-0.0008141211583279073,
-0.0487440824508667,
0.012847818434238434,
-0.029548218473792076,
0.07841724902391434,
-0.02906130999326706,
0.14496572315692902,
0.012137218378484249,
-0.029361413791775703,
-0.0756242498755455,
0.03895791992545128,
0.025698240846395493,
-0.023142307996749878,
-0.054554373025894165,
0.0046921903267502785,
0.023646147921681404,
-0.006090340204536915,
-0.013665683567523956,
-0.05054721608757973,
-0.049316830933094025,
-0.06718342006206512,
0.0001234931405633688,
-0.036372698843479156,
-0.028900709003210068,
0.02847300097346306,
-0.022112702950835228,
-0.03873012214899063,
0.006759404670447111,
-0.033264316618442535,
0.031050613150000572,
0.08903665095567703,
0.034366052597761154,
-0.0014945600414648652,
-0.01721685752272606,
0.025727281346917152,
-0.04489405080676079,
-0.0033884572330862284,
-0.00037386719486676157,
-0.11006414145231247,
-0.08654825389385223,
-0.03041255846619606,
0.004575608763843775,
0.048781417310237885,
-0.04875423386693001,
0.007969699800014496,
-0.04160165414214134,
0.019382348284125328,
-0.01309303566813469,
-0.013718710280954838,
0.03396640717983246,
-0.06869197636842728,
0.16892856359481812,
-0.04536838456988335,
-0.03216864541172981,
-0.007419194094836712,
0.026447877287864685,
-0.02030385099351406,
-0.016157450154423714,
-0.0655922144651413,
0.0221058651804924,
0.016973020508885384,
0.0016717022517696023,
0.050593938678503036,
0.04152441769838333,
0.010915243998169899,
-0.006944605614989996,
-0.04349583014845848,
-0.02795441262423992,
0.015914829447865486,
-0.042875126004219055,
0.041092321276664734,
-0.02298787049949169,
0.004368647933006287,
0.0009858733974397182,
0.0007139150402508676,
0.041381560266017914,
-0.24926364421844482,
0.030036412179470062,
0.01693960279226303,
0.03525489941239357,
0.04676505923271179,
-0.0002159542345907539,
-0.009286037646234035,
0.04085865616798401,
0.061060577630996704,
0.014110956341028214,
-0.00952867977321148,
0.0014170034555718303,
0.04350026324391365,
-0.012623116374015808,
-0.03846575692296028,
-0.02114907279610634,
0.0337032712996006,
0.00724978931248188,
0.037525925785303116,
-0.016722965985536575,
0.05467679351568222,
-0.013710525818169117,
0.14812812209129333,
0.020897196605801582,
-0.021930692717432976,
0.03238082677125931,
0.01531124860048294,
0.06883078068494797,
0.029347170144319534,
0.019927239045500755,
0.08404167741537094,
-0.016017574816942215,
0.062883660197258,
0.018103105947375298,
0.05449376627802849,
-0.015387006103992462,
-0.0377652682363987,
0.007511219009757042,
0.01834358274936676,
-0.020068662241101265,
-0.024805711582303047,
-0.007471353281289339,
-0.03448915854096413,
-0.056130677461624146,
0.028783997520804405,
-0.033983852714300156,
-0.013145309872925282,
-0.13337740302085876,
0.036738425493240356,
-0.02092331275343895,
0.056719403713941574,
-0.016734736040234566,
0.059099987149238586,
0.06055412068963051,
0.01576848328113556,
0.0017727160593494773,
-0.010691579431295395,
0.03639807924628258,
0.037774961441755295,
-0.020438268780708313,
0.062373846769332886,
0.023737937211990356,
-0.04800103232264519,
0.07112203538417816,
0.047277484089136124
] |
PMID:25065 | Poly(adenosine dephosphate ribose) metabolism and regulation of myocardial cell growth by oxygen. | Control of the rate of cardiac cell division by oxygen occurs most probably by altering the redox state of a control substance, e.g. NAD(+)right harpoon over left harpoonNADH. NAD(+) (and not NADH) forms poly(ADP-ribose), an inhibitor of DNA synthesis, in a reaction catalysed by poly(ADP-ribose) polymerase. Lower partial pressure of oxygen, which increases the rate of division, would shift NAD(+)-->NADH, decrease poly(ADP-ribose) synthesis, and increase DNA synthesis. Chick-embryo heart cells grown in culture in 20% O(2) (in which they divide more slowly than in 5% O(2)) did exhibit greater poly(ADP-ribose) polymerase activity (+83%, P<0.001) than when grown in 5% O(2). Reaction product was identified as poly(ADP-ribose) by its insensitivity to deoxyribonuclease, ribonuclease, NAD glycohydrolase, Pronase, trypsin and micrococcal nuclease, and by its complete digestion with snake-venom phosphodiesterase to phosphoribosyl-AMP and AMP. Isolation of these digestion products by Dowex 1 (formate form) column chromatography and paper chromatography allowed calculation of average poly(ADP-ribose) chain length, which was 15-26% greater in 20% than in 5% O(2). Thus in 20% O(2) the increase in poly(ADP-ribose) formation results from chain elongation. Formation of new chains also occurs, probably to an even greater degree than chain elongation. Additionally, poly(ADP-ribose) polymerase has very different K(m) and V(max.) values and pH optima in 20% and 5% O(2). These data suggest that poly(ADP-ribose) metabolism participates in the regulation of heart-cell division by O(2), probably by several different mechanisms. | Poly(adenosine dephosphate ribose) metabolism and regulation of myocardial cell growth by oxygen. Control of the rate of cardiac cell division by oxygen occurs most probably by altering the redox state of a control substance, e.g. NAD(+)right harpoon over left harpoonNADH. NAD(+) (and not NADH) forms poly(ADP-ribose), an inhibitor of DNA synthesis, in a reaction catalysed by poly(ADP-ribose) polymerase. Lower partial pressure of oxygen, which increases the rate of division, would shift NAD(+)-->NADH, decrease poly(ADP-ribose) synthesis, and increase DNA synthesis. Chick-embryo heart cells grown in culture in 20% O(2) (in which they divide more slowly than in 5% O(2)) did exhibit greater poly(ADP-ribose) polymerase activity (+83%, P<0.001) than when grown in 5% O(2). Reaction product was identified as poly(ADP-ribose) by its insensitivity to deoxyribonuclease, ribonuclease, NAD glycohydrolase, Pronase, trypsin and micrococcal nuclease, and by its complete digestion with snake-venom phosphodiesterase to phosphoribosyl-AMP and AMP. Isolation of these digestion products by Dowex 1 (formate form) column chromatography and paper chromatography allowed calculation of average poly(ADP-ribose) chain length, which was 15-26% greater in 20% than in 5% O(2). Thus in 20% O(2) the increase in poly(ADP-ribose) formation results from chain elongation. Formation of new chains also occurs, probably to an even greater degree than chain elongation. Additionally, poly(ADP-ribose) polymerase has very different K(m) and V(max.) values and pH optima in 20% and 5% O(2). These data suggest that poly(ADP-ribose) metabolism participates in the regulation of heart-cell division by O(2), probably by several different mechanisms. | [
-0.057926516979932785,
-0.006658685393631458,
0.040546219795942307,
0.02771024778485298,
0.02260691486299038,
0.032647982239723206,
0.008265258744359016,
-0.0010035625891759992,
-0.04288986325263977,
-0.02093622088432312,
0.08572197705507278,
-0.0635748952627182,
-0.0062415883876383305,
-0.022982606664299965,
-0.04526403173804283,
-0.03888719528913498,
-0.04567580670118332,
-0.0315999798476696,
-0.0723835900425911,
0.009459225460886955,
0.03228089585900307,
0.02332759089767933,
-0.027887700125575066,
-0.04737762361764908,
0.05167612060904503,
0.03575507178902626,
0.009067578241229057,
-0.000855022284667939,
-0.0692284107208252,
-0.19184938073158264,
-0.02313263528048992,
0.013223166577517986,
0.030516907572746277,
-0.03168821707367897,
-0.0514250285923481,
0.008865774609148502,
-0.017073186114430428,
0.020781509578227997,
-0.07428179681301117,
0.06861267238855362,
-0.031659338623285294,
0.007639331743121147,
-0.005381432827562094,
0.013947517611086369,
0.02140321582555771,
0.03488772362470627,
-0.040954429656267166,
-0.010042916052043438,
0.0565672442317009,
0.01709171198308468,
-0.013478117994964123,
0.0002487491874489933,
-0.039294809103012085,
-0.005512499250471592,
0.026436103507876396,
-0.05966919660568237,
0.03438042849302292,
0.043035075068473816,
0.013952947221696377,
-0.03818657249212265,
0.021396931260824203,
0.018040049821138382,
-0.11048631370067596,
0.05127784237265587,
0.08612057566642761,
-0.015153944492340088,
0.01265177596360445,
-0.007035804446786642,
0.01232541911303997,
0.019308337941765785,
-0.08841673284769058,
0.03466694802045822,
0.019602853804826736,
0.018663043156266212,
0.004338573664426804,
-0.030051719397306442,
-0.02356616221368313,
-0.0055736638605594635,
0.06474703550338745,
0.018990667536854744,
-0.029286038130521774,
-0.013133845292031765,
-0.041792746633291245,
-0.021085212007164955,
-0.06434474885463715,
0.030742693692445755,
0.05040588229894638,
-0.08004685491323471,
0.010549012571573257,
0.02517189085483551,
-0.020368484780192375,
-0.051861245185136795,
-0.0028821418527513742,
-0.043929941952228546,
-0.08457119762897491,
0.014035062864422798,
0.0025025582872331142,
0.024997621774673462,
0.021087704226374626,
0.3638518154621124,
-0.022493133321404457,
0.05783515423536301,
-0.06951954215765,
-0.03218197077512741,
-0.025990454480051994,
-0.10519438236951828,
-0.023308491334319115,
0.06039922311902046,
0.02116016484797001,
0.07810305804014206,
0.028714830055832863,
0.02514462172985077,
-0.0343688428401947,
-0.010592002421617508,
0.03540938347578049,
0.014124810695648193,
-0.036327801644802094,
0.011347115971148014,
-0.06877662241458893,
0.04481033608317375,
-0.006374388933181763,
-0.021450206637382507,
0.0021617363672703505,
0.002446515252813697,
0.028665876016020775,
0.03702419996261597,
-0.03292703628540039,
0.0823403000831604,
0.06923764944076538,
-0.007927960716187954,
0.04774945229291916,
0.04766837880015373,
-0.043089985847473145,
0.02100101113319397,
-0.006483992096036673,
-0.03372328355908394,
-0.017920196056365967,
-0.004299416206777096,
0.056195322424173355,
-0.036137040704488754,
-0.05032084137201309,
0.00262406631372869,
-0.02786422148346901,
-0.028836693614721298,
-0.016585838049650192,
0.10532430559396744,
0.020469140261411667,
0.11087758839130402,
-0.017145631834864616,
-0.005331012886017561,
0.002077308716252446,
0.0639139860868454,
-0.03142682835459709,
0.025797342881560326,
-0.02332405559718609,
0.0005180622101761401,
-0.03354987874627113,
-0.003808849724009633,
0.07158900797367096,
-0.06461729109287262,
-0.10868635028600693,
0.01805153116583824,
0.05158097669482231,
0.05873964726924896,
0.007868638262152672,
-0.016091398894786835,
-0.04420547932386398,
0.06316621601581573,
0.012673275545239449,
-0.05018516257405281,
0.017831599339842796,
-0.0350656658411026,
-0.0016201675171032548,
-0.04928961396217346,
0.015941482037305832,
-0.03964803367853165,
0.019294455647468567,
-0.004077764693647623,
0.052026860415935516,
-0.014544136822223663,
0.030486030504107475,
-0.020110726356506348,
0.024982215836644173,
0.0027907288167625666,
0.02773088589310646,
-0.03687907010316849,
-0.06328346580266953,
0.049381960183382034,
-0.016732610762119293,
0.03453903645277023,
-0.0032163511496037245,
-0.0032366756349802017,
-0.11207905411720276,
0.02828197553753853,
-0.07299881428480148,
-0.053351663053035736,
0.01793554238975048,
0.067625992000103,
-0.03670957311987877,
0.028995027765631676,
0.07938835024833679,
0.02727401629090309,
0.047630030661821365,
0.05941716581583023,
-0.04041416198015213,
0.0010661210399121046,
0.021057995036244392,
0.05098875239491463,
0.017958497628569603,
0.022134777158498764,
-0.06194533035159111,
-0.0034962843637913465,
-0.06201815605163574,
0.04797331988811493,
-0.06335090100765228,
0.029414677992463112,
-0.026907647028565407,
0.024446144700050354,
-0.012722610495984554,
0.050747089087963104,
0.009540700353682041,
-0.08350467681884766,
0.020341046154499054,
-0.2972067594528198,
0.0022377343848347664,
0.03029273822903633,
-0.03575276583433151,
0.06919890642166138,
0.02849576808512211,
0.02552127093076706,
0.048934753984212875,
-0.015544317662715912,
-0.048224471509456635,
-0.020115353167057037,
0.04107535257935524,
-0.047638602554798126,
-0.068516306579113,
0.0018744453554973006,
0.023345207795500755,
-0.004374508745968342,
-0.023828711360692978,
-0.03285045921802521,
0.03978728502988815,
0.007097784895449877,
-0.006056466605514288,
-0.006394257768988609,
0.023757360875606537,
0.04573773592710495,
-0.01401245966553688,
0.08626335859298706,
0.008471719920635223,
0.023681990802288055,
0.012448299676179886,
0.017513656988739967,
0.024746326729655266,
-0.026312902569770813,
-0.08098609745502472,
-0.00792141817510128,
-0.012625597417354584,
-0.031918108463287354,
-0.04226567596197128,
-0.02593877725303173,
-0.0010365498019382358,
0.0017131561180576682,
0.04770887270569801,
-0.0242643840610981,
-0.04771706834435463,
0.005555879790335894,
-0.04190430790185928,
-0.010732730850577354,
0.047277726233005524,
0.02464541420340538,
-0.014847583137452602,
0.017281202599406242,
0.023722512647509575,
0.009859457612037659,
-0.027980275452136993,
0.0422518253326416,
-0.04126874729990959,
-0.04533326253294945,
0.0026457568164914846,
-0.06669270992279053,
-0.03531403839588165,
0.021691463887691498,
0.005073891952633858,
-0.0559811033308506,
0.02561260014772415,
-0.0008487501763738692,
0.0607304647564888,
0.002419972326606512,
-0.02407235838472843,
0.02176693268120289,
0.002958877244964242,
0.03425664082169533,
0.1157718300819397,
-0.026409560814499855,
0.01849868893623352,
0.09455285966396332,
0.012181038036942482,
-0.08469521999359131,
-0.021828165277838707,
-0.04806985706090927,
-0.04015086591243744,
0.033607367426157,
-0.06298011541366577,
0.039020247757434845,
-0.01887502335011959,
-0.05827445536851883,
-0.006183647084981203,
0.018319252878427505,
0.019226040691137314,
0.029388783499598503,
-0.015239045955240726,
0.006085252854973078,
-0.020783806219697,
0.003947798628360033,
-0.03728803992271423,
0.03253380209207535,
0.004712224472314119,
-0.2591371238231659,
0.0007018752512522042,
0.0365939624607563,
-0.015300248749554157,
-0.07030957192182541,
0.04429980367422104,
0.02038036845624447,
0.01379444170743227,
0.01943807490170002,
0.035463713109493256,
-0.0017732273554429412,
0.01823343336582184,
0.06823033094406128,
0.08261872082948685,
-0.04071293771266937,
0.05580221489071846,
0.05224039778113365,
-0.026118025183677673,
0.01897665485739708,
-0.03576676547527313,
0.022701937705278397,
-0.06426246464252472,
0.1295815110206604,
0.020809434354305267,
0.010738000273704529,
0.020633557811379433,
-0.004215538967400789,
0.06903131306171417,
-0.03812212496995926,
0.022406436502933502,
-0.007693228777498007,
0.04972349852323532,
0.05080948397517204,
-0.014777244068682194,
0.039348967373371124,
0.043847426772117615,
-0.04509760066866875,
0.048083871603012085,
-0.025929704308509827,
0.03677142411470413,
-0.02534150891005993,
-0.006112703122198582,
-0.07765813916921616,
-0.04659215360879898,
0.1041937917470932,
-0.06502339243888855,
-0.020967455580830574,
-0.05217694118618965,
0.028422577306628227,
0.028930721804499626,
-0.02391638606786728,
0.0009353108471259475,
0.06289392709732056,
0.03444025665521622,
-0.006978210993111134,
-0.005693435203284025,
-0.01061644684523344,
-0.031084606423974037,
0.05474398285150528,
-0.016720326617360115,
0.014248465187847614,
-0.03382324054837227,
-0.029511451721191406,
0.16999267041683197,
0.046968892216682434
] |
PMID:25066 | Intestinal neuraminidase activity of suckling rats and other mammals. Relationship to the sialic acid content of milk. | 1. The neuraminidase activity of homogenates of the mucosa of the middle and distal thirds of the small intestine of rats increased about 5-fold between birth and 4 to 8 days of age, and then gradually declined to the much lower adult activity by 24 days. No comparable changes occurred in the proximal third. 2. In 8-day-old rats, the neuraminidase activity of the middle and distal thirds of the small intestine was about 10 times greater than that of the proximal third, 20 times greater than that of the colon and at least 100 times greater than that of the liver, brain, gastric mucosa or pancreas. 3. In all other species investigated (mice, rabbits, cats and guinea pigs), the neuraminidase activity of the middle and distal thirds of the small intestine was greater in suckling animals than in adults. 4. The sialic acid content of rat milk increased about 2-fold between birth and 8 days post partum and then declined. 5. There was a highly significant positive correlation between the intestinal neuraminidase activity of suckling animals of various species and ages and the sialic acid content of milk obtained from the corresponding species and stage of lactation. 6. It is suggested that the intestinal neuraminidase of suckling mammals functions primarily to remove sialic acid from various components of milk, thus providing sialic acid for the synthesis of sialoglycoproteins and gangliosides by the young. | Intestinal neuraminidase activity of suckling rats and other mammals. Relationship to the sialic acid content of milk. 1. The neuraminidase activity of homogenates of the mucosa of the middle and distal thirds of the small intestine of rats increased about 5-fold between birth and 4 to 8 days of age, and then gradually declined to the much lower adult activity by 24 days. No comparable changes occurred in the proximal third. 2. In 8-day-old rats, the neuraminidase activity of the middle and distal thirds of the small intestine was about 10 times greater than that of the proximal third, 20 times greater than that of the colon and at least 100 times greater than that of the liver, brain, gastric mucosa or pancreas. 3. In all other species investigated (mice, rabbits, cats and guinea pigs), the neuraminidase activity of the middle and distal thirds of the small intestine was greater in suckling animals than in adults. 4. The sialic acid content of rat milk increased about 2-fold between birth and 8 days post partum and then declined. 5. There was a highly significant positive correlation between the intestinal neuraminidase activity of suckling animals of various species and ages and the sialic acid content of milk obtained from the corresponding species and stage of lactation. 6. It is suggested that the intestinal neuraminidase of suckling mammals functions primarily to remove sialic acid from various components of milk, thus providing sialic acid for the synthesis of sialoglycoproteins and gangliosides by the young. | [
-0.003452339908108115,
-0.045055363327264786,
0.03924361988902092,
-0.01635381206870079,
-0.001187711488455534,
-0.022208133712410927,
0.0713251605629921,
0.09514401108026505,
-0.07959344238042831,
-0.036940086632966995,
0.012349662370979786,
-0.11673678457736969,
0.02528027445077896,
0.018982309848070145,
-0.0268965233117342,
-0.02677876688539982,
-0.02333828993141651,
-0.020411105826497078,
-0.10152163356542587,
-0.018207574263215065,
-0.002159407362341881,
-0.034540239721536636,
0.05168285593390465,
0.0032807975076138973,
0.07692927867174149,
0.030811142176389694,
-0.0044188471511006355,
-0.023987310007214546,
-0.056692250072956085,
-0.1771344393491745,
0.00746654300019145,
-0.02384316176176071,
0.04646209627389908,
-0.00497759273275733,
-0.03374355658888817,
0.019953500479459763,
0.0024178107269108295,
0.017870595678687096,
-0.0062523894011974335,
0.0820116475224495,
0.08846501260995865,
0.03591014817357063,
0.012138448655605316,
0.01426211278885603,
-0.008560210466384888,
0.022016355767846107,
-0.0773085206747055,
-0.0012653331505134702,
0.07385431975126266,
0.018470972776412964,
-0.01148794312030077,
-0.023136531934142113,
0.011554419994354248,
0.07356725633144379,
0.021562160924077034,
0.010229447856545448,
0.02766113542020321,
0.018298493698239326,
0.024667086079716682,
0.06828317046165466,
0.002216006861999631,
-0.0036758463829755783,
-0.14937378466129303,
0.08392611145973206,
0.04528817906975746,
0.002649501198902726,
-0.03835585340857506,
-0.050614360719919205,
0.001111385296098888,
0.022229261696338654,
-0.04896872863173485,
0.08826318383216858,
-0.006330379284918308,
-0.028422458097338676,
-0.0050505176186561584,
-0.059852056205272675,
0.039139069616794586,
0.027926471084356308,
0.01965837925672531,
0.02984504960477352,
0.031572673469781876,
0.05109206214547157,
0.0351349301636219,
-0.03454974666237831,
-0.009901631623506546,
0.008168154396116734,
-0.049566589295864105,
-0.023576732724905014,
-0.010280022397637367,
0.04933686926960945,
-0.035827234387397766,
-0.03783136606216431,
-0.051682014018297195,
-0.02135230414569378,
-0.0589730441570282,
0.0573062039911747,
-0.0024913079105317593,
-0.023739660158753395,
0.029304180294275284,
0.36080729961395264,
-0.04142492264509201,
0.0044089676812291145,
-0.05297159031033516,
-0.032603032886981964,
-0.006903157569468021,
0.02682102471590042,
-0.01667221076786518,
0.01908218301832676,
-0.006273997947573662,
0.07603750377893448,
0.0647820457816124,
0.06555448472499847,
-0.023573456332087517,
-0.057134971022605896,
0.019315380603075027,
-0.010750547051429749,
0.041222136467695236,
-0.004993645008653402,
-0.0284426286816597,
0.06461667269468307,
-0.004499735310673714,
0.02227950654923916,
-0.040228471159935,
-0.041343361139297485,
0.04835568740963936,
0.026816880330443382,
-0.012683798559010029,
0.08277809619903564,
0.0235576331615448,
-0.02413308434188366,
0.020597415044903755,
0.02090470679104328,
-0.01435818150639534,
0.009898340329527855,
0.033612944185733795,
-0.05981849879026413,
0.007604539860039949,
0.016184372827410698,
0.012715238146483898,
-0.030332522466778755,
-0.05625378340482712,
-0.056151047348976135,
0.011035426519811153,
-0.07957006990909576,
-0.0022054449655115604,
0.10250075906515121,
0.006702781654894352,
0.004726937972009182,
-0.02680407650768757,
-0.0041512358002364635,
0.01800922490656376,
0.07310717552900314,
0.014794019050896168,
0.04944688826799393,
0.027653060853481293,
0.01885322853922844,
-0.013843953609466553,
-0.03917688503861427,
-0.043192457407712936,
-0.004173498600721359,
-0.068824902176857,
-0.017770174890756607,
-0.048217274248600006,
0.03516215458512306,
0.04928898438811302,
-0.0013347751228138804,
-0.0065637994557619095,
0.008554457686841488,
0.03359396383166313,
0.005978041794151068,
-0.027251245453953743,
0.039732787758111954,
-0.05703302100300789,
-0.056005049496889114,
-0.0382218062877655,
0.02104019746184349,
0.014879309572279453,
0.04006548225879669,
0.03178868815302849,
0.03248210251331329,
-0.0010921475477516651,
-0.05411626771092415,
-0.003439920488744974,
0.014389412477612495,
-0.00853271596133709,
-0.01816776767373085,
0.006169232539832592,
0.02078736387193203,
0.013602341525256634,
0.0030611460097134113,
-0.03781912475824356,
-0.03641558811068535,
-0.08290745317935944,
-0.021121080964803696,
-0.032064467668533325,
-0.020353887230157852,
-0.010826864279806614,
0.01618364453315735,
-0.00981739442795515,
-0.013628083281219006,
0.04033900424838066,
-0.025401629507541656,
0.05380866304039955,
0.019670264795422554,
0.06012098863720894,
-0.014075962826609612,
0.028742805123329163,
0.01762894168496132,
0.07816292345523834,
-0.011079833842813969,
0.020491205155849457,
0.0047256373800337315,
-0.046623192727565765,
0.02412664145231247,
-0.05505109950900078,
0.06004561111330986,
0.014042355120182037,
0.0009467065683566034,
0.051946450024843216,
0.04501229152083397,
-0.0037941704504191875,
-0.022877143695950508,
-0.0035087952855974436,
-0.291487455368042,
-0.025476763024926186,
-0.017054185271263123,
-0.06805259734392166,
0.05193624645471573,
-0.07375726103782654,
-0.0002509745245333761,
-0.04921850934624672,
0.047480881214141846,
-0.0479593500494957,
0.0513654425740242,
0.04534027725458145,
-0.01590915583074093,
0.021081876009702682,
0.004626784007996321,
0.055560845881700516,
0.0462445504963398,
-0.002061237348243594,
0.0491737499833107,
0.043602559715509415,
0.008002743124961853,
-0.026425637304782867,
0.09486834704875946,
0.00761061254888773,
0.017927132546901703,
0.0045985267497599125,
0.12860937416553497,
0.045128948986530304,
0.03652206063270569,
0.02288905531167984,
0.0025142652448266745,
0.05374594032764435,
-0.032654520124197006,
-0.06622569262981415,
-0.021998021751642227,
-0.02851395681500435,
-0.03216097503900528,
-0.016648581251502037,
-0.0523359552025795,
0.0143211605027318,
-0.028552018105983734,
0.007392814848572016,
0.041647735983133316,
-0.03728706017136574,
-0.02898963913321495,
-0.023293770849704742,
0.02308139204978943,
0.010116463527083397,
0.007710738573223352,
-0.04750332236289978,
0.022369496524333954,
-0.01683245599269867,
0.007011559791862965,
0.038113661110401154,
-0.04577747359871864,
0.010036081075668335,
-0.002003564964979887,
0.025682223960757256,
-0.06858334690332413,
0.0341460257768631,
-0.041619814932346344,
0.0018970748642459512,
-0.027381686493754387,
-0.023449981585144997,
-0.03794267773628235,
0.055096399039030075,
-0.009761709719896317,
-0.03085276298224926,
-0.009293313138186932,
0.0651462972164154,
0.010745750740170479,
0.12971948087215424,
-0.07984548807144165,
-0.01813698187470436,
-0.021509991958737373,
0.038843512535095215,
-0.028589550405740738,
-0.07812575250864029,
-0.02977124974131584,
0.030748093500733376,
-0.01953248120844364,
-0.08151483535766602,
0.08076050877571106,
-0.017151841893792152,
0.030698714777827263,
-0.005891365930438042,
0.025713695213198662,
-0.015813741832971573,
0.03981444239616394,
0.022238554432988167,
0.045852985233068466,
0.02144431136548519,
-0.0476117841899395,
-0.011381630785763264,
0.06010193005204201,
0.043732352554798126,
-0.29826024174690247,
0.055727940052747726,
0.0056352135725319386,
-0.05151590704917908,
0.00715277623385191,
-0.018817905336618423,
0.013349812477827072,
-0.05107738822698593,
-0.03465944156050682,
0.04384709894657135,
0.04021897539496422,
0.038165729492902756,
0.059130068868398666,
0.01964516006410122,
-0.007568787783384323,
-0.015047610737383366,
-0.004021733533591032,
0.03889526426792145,
0.053855668753385544,
-0.02279643528163433,
0.03961426764726639,
-0.06428994983434677,
0.16503506898880005,
0.04396124184131622,
-0.017773155122995377,
0.021953463554382324,
-0.03315792977809906,
0.020271800458431244,
0.008351887576282024,
-0.029585886746644974,
-0.016715647652745247,
0.01390787959098816,
0.06382894515991211,
-0.014236228540539742,
0.002296601887792349,
0.07082885503768921,
-0.023015305399894714,
-0.04871595650911331,
-0.023569485172629356,
0.03947881609201431,
-0.03275599703192711,
0.007564240135252476,
-0.07260502874851227,
-0.04293302074074745,
0.07389312982559204,
-0.11990009993314743,
-0.03324822336435318,
-0.027895908802747726,
0.018078884109854698,
0.009183444082736969,
-0.04997559264302254,
-0.07814030349254608,
0.025069458410143852,
0.00003774031574721448,
0.03127296268939972,
-0.04709681496024132,
-0.014458054676651955,
-0.039912547916173935,
-0.050612423568964005,
-0.015188652090728283,
-0.001211249502375722,
-0.02168760821223259,
-0.04871401563286781,
0.001868750900030136,
-0.0013223098358139396
] |
PMID:25070 | [Reaction kinetic studies on the formation of n-nitrosoephedrine in vitro and in vivo (author's transl)]. | N-Nitrosoephedrine was synthesized at a high yield (greater than 90%). The influence of pH on the reaction process (ephedrine sulfate + nitrous acid in hydrochloric acid) was investigated and the pH-optimum determined. Furthermore the influence of coffee, ascorbic acid, citric acid, cyclamate/saccharin (10:1), smoke condensate of cigarettes, glucose and ethanol on the reaction process was also determined. The tests with animals (rats) showed that N-nitrosoephedrine could be detected in serum after the oral application of ephedrine sulfate and sodium nitrite. | [Reaction kinetic studies on the formation of n-nitrosoephedrine in vitro and in vivo (author's transl)]. N-Nitrosoephedrine was synthesized at a high yield (greater than 90%). The influence of pH on the reaction process (ephedrine sulfate + nitrous acid in hydrochloric acid) was investigated and the pH-optimum determined. Furthermore the influence of coffee, ascorbic acid, citric acid, cyclamate/saccharin (10:1), smoke condensate of cigarettes, glucose and ethanol on the reaction process was also determined. The tests with animals (rats) showed that N-nitrosoephedrine could be detected in serum after the oral application of ephedrine sulfate and sodium nitrite. | [
-0.08185341209173203,
0.06323660165071487,
0.003492669900879264,
-0.010918364860117435,
0.030629126355051994,
0.023809785023331642,
0.05080127716064453,
0.04738666117191315,
-0.06514012068510056,
0.016082512214779854,
0.00007375402492471039,
-0.04432017356157303,
-0.08366598188877106,
0.02333846502006054,
-0.048309292644262314,
-0.04250452294945717,
0.016629762947559357,
-0.048023831099271774,
-0.07295529544353485,
-0.003899927483871579,
-0.04275057464838028,
0.004361252766102552,
0.048442378640174866,
0.031538866460323334,
0.04729645326733589,
0.001912991632707417,
-0.0022161705419421196,
0.02314796857535839,
-0.02255292609333992,
-0.1866782158613205,
0.06415651738643646,
0.017011817544698715,
0.0347776897251606,
-0.03794841840863228,
-0.05073132365942001,
0.06653373688459396,
-0.024453362450003624,
0.012465709820389748,
-0.01053048949688673,
0.013172801584005356,
0.04906969889998436,
0.020290443673729897,
0.01473724190145731,
0.01593083329498768,
0.09216754883527756,
0.06293050944805145,
-0.030080392956733704,
0.010159100405871868,
0.09935426712036133,
-0.0040634674951434135,
0.006190937478095293,
-0.054058484733104706,
0.016622696071863174,
0.01957547478377819,
0.018074939027428627,
-0.002784674521535635,
-0.006180605385452509,
-0.029000328853726387,
0.036370083689689636,
0.039310578256845474,
0.01801733858883381,
0.032628849148750305,
-0.19308800995349884,
0.02543996460735798,
0.0449669249355793,
-0.022953268140554428,
-0.025205880403518677,
0.029621168971061707,
0.05966569855809212,
0.13805176317691803,
-0.02443813532590866,
0.05533263087272644,
-0.0034018398728221655,
-0.001600927789695561,
0.02652081847190857,
0.05951409041881561,
0.027072643861174583,
0.012992781586945057,
-0.00492082629352808,
-0.027766918763518333,
-0.010199676267802715,
0.06632120162248611,
-0.0212620310485363,
0.0425991527736187,
0.05537712201476097,
-0.014171023853123188,
0.025874275714159012,
0.029742475599050522,
0.0030696564354002476,
-0.0011185579933226109,
0.019000543281435966,
-0.03902868926525116,
-0.03626628965139389,
-0.05828598141670227,
-0.07166635990142822,
-0.02011680230498314,
-0.007606555707752705,
-0.023271113634109497,
0.0024033463560044765,
0.3501127362251282,
-0.03880520164966583,
-0.003360328497365117,
-0.04855922982096672,
-0.019401120021939278,
-0.014811655506491661,
-0.034493621438741684,
0.002245187060907483,
-0.019856780767440796,
0.004762853495776653,
0.04197604954242706,
0.05748622119426727,
-0.015804780647158623,
0.05784591659903526,
-0.09126894921064377,
0.012483947910368443,
-0.007450406905263662,
0.07667405158281326,
-0.004609368741512299,
-0.0021494568791240454,
0.0070815482176840305,
-0.004354282282292843,
0.0686957985162735,
0.006059569772332907,
-0.047034285962581635,
-0.009395428001880646,
-0.006584596820175648,
-0.028775222599506378,
0.05892866477370262,
0.03932406008243561,
0.021308215335011482,
-0.013226563110947609,
-0.04699315130710602,
0.02208048477768898,
0.015409824438393116,
-0.01876382902264595,
-0.020702719688415527,
0.07376575469970703,
0.048855382949113846,
0.012538443319499493,
-0.013497560285031796,
-0.02601141855120659,
-0.0035048306453973055,
-0.043789591640233994,
-0.09896311908960342,
-0.07249131798744202,
0.11103566735982895,
0.017663121223449707,
0.004316593520343304,
-0.021197985857725143,
-0.012676877900958061,
-0.0030989395454525948,
0.020638858899474144,
-0.06766275316476822,
0.017815081402659416,
-0.02854246273636818,
-0.011730668134987354,
-0.023928038775920868,
0.011300534941256046,
-0.008162268437445164,
0.012303810566663742,
-0.06075439229607582,
-0.06816795468330383,
0.04194296523928642,
0.040583185851573944,
-0.02748209424316883,
-0.012726877816021442,
-0.0649159774184227,
0.011831975542008877,
0.007129136472940445,
-0.007257342804223299,
0.009212339296936989,
-0.028407294303178787,
0.023009341210126877,
-0.004079756326973438,
-0.0236523300409317,
0.04883787781000137,
0.03798412159085274,
0.032969336956739426,
0.02221609465777874,
0.0013529090210795403,
0.07666667550802231,
0.03158290684223175,
-0.01653267629444599,
-0.031214063987135887,
0.03816278278827667,
-0.06263390928506851,
-0.01575874164700508,
-0.002404983853921294,
-0.02163974568247795,
0.05098196864128113,
-0.07151929289102554,
-0.02275201305747032,
-0.10040628910064697,
0.07442136108875275,
-0.029468541964888573,
0.008273562416434288,
-0.008800486102700233,
0.026405850425362587,
0.003707455936819315,
-0.02533811330795288,
0.012608269229531288,
0.012085936963558197,
0.0012292785104364157,
0.034736525267362595,
0.07285109162330627,
0.025210965424776077,
-0.011150436475872993,
0.02940521575510502,
0.06647569686174393,
-0.031374186277389526,
0.00344138708896935,
0.05777368322014809,
-0.030069155618548393,
0.009204977191984653,
-0.05650204047560692,
-0.015948569402098656,
-0.03425697982311249,
0.060990478843450546,
0.06070072576403618,
-0.024052172899246216,
0.03270505368709564,
-0.021220697090029716,
-0.011382576078176498,
-0.28053978085517883,
0.012875229120254517,
0.04057798534631729,
0.04487283155322075,
0.0006786716403439641,
-0.0794663280248642,
0.026460016146302223,
-0.05120868235826492,
0.028766846284270287,
-0.049208614975214005,
0.06358649581670761,
0.06704360246658325,
-0.03514837473630905,
-0.0043812901712954044,
0.0029952628538012505,
0.020037690177559853,
0.027407513931393623,
-0.00822411384433508,
0.048686787486076355,
0.03919372707605362,
0.030246052891016006,
-0.060100119560956955,
0.0051870220340788364,
-0.01150505430996418,
0.0515705868601799,
0.011544579640030861,
0.04929383844137192,
-0.013004358857870102,
-0.011120650917291641,
-0.03770105913281441,
0.027127202600240707,
0.04844586178660393,
-0.07968514412641525,
-0.12085554748773575,
-0.02247295156121254,
-0.008341852575540543,
0.013156486675143242,
0.0020882408134639263,
0.025554601103067398,
0.008173040114343166,
-0.02630651369690895,
-0.04332539066672325,
0.04979030787944794,
-0.04999551177024841,
-0.006039232946932316,
-0.017830898985266685,
-0.03200734034180641,
0.0356881208717823,
-0.04902578517794609,
-0.02901073917746544,
0.044374577701091766,
0.022378934547305107,
0.01827337220311165,
-0.049085646867752075,
0.013261537998914719,
-0.029638350009918213,
-0.03848671540617943,
0.030020596459507942,
-0.07035093009471893,
-0.058023903518915176,
-0.0071266512386500835,
0.005936312954872847,
-0.003262941027060151,
-0.006305839400738478,
0.056523047387599945,
-0.004327104892581701,
0.014265956357121468,
-0.014224491082131863,
-0.014151111245155334,
0.06794832646846771,
0.05421081930398941,
0.13200025260448456,
-0.08842355757951736,
0.012778148055076599,
0.06571418046951294,
0.014092533849179745,
-0.05272212252020836,
-0.005913841538131237,
-0.0635996088385582,
-0.008077748119831085,
-0.0013052060967311263,
-0.001650810008868575,
0.0625431016087532,
-0.018924089148640633,
-0.03474725782871246,
0.017425984144210815,
0.022015534341335297,
-0.0385686419904232,
0.04836440086364746,
-0.02800273522734642,
0.023906240239739418,
0.033847130835056305,
-0.02920686826109886,
-0.07028904557228088,
0.06058969348669052,
0.08320144563913345,
-0.29013657569885254,
-0.04221450537443161,
0.0026595068629831076,
0.026510650292038918,
0.01853892020881176,
-0.016272788867354393,
0.04692738875746727,
-0.03448113426566124,
-0.01028959546238184,
0.02897350676357746,
-0.014058488421142101,
-0.003364509204402566,
0.013837587088346481,
0.04720941558480263,
0.02889634482562542,
-0.0015788915334269404,
0.01998167112469673,
-0.04646763950586319,
0.010752291418612003,
-0.050660308450460434,
0.014452940784394741,
-0.042203161865472794,
0.1386837512254715,
-0.047783154994249344,
-0.03619535639882088,
0.04910239204764366,
-0.024483438581228256,
0.028127506375312805,
-0.05286264419555664,
0.02495303563773632,
-0.022422371432185173,
-0.05402107909321785,
0.06274925172328949,
-0.061463065445423126,
0.008346478454768658,
0.12413046509027481,
-0.061058130115270615,
-0.008904339745640755,
-0.062142398208379745,
-0.016893111169338226,
-0.02553117647767067,
0.02047846093773842,
0.004401293583214283,
-0.016155464574694633,
0.04332815855741501,
-0.03464909642934799,
-0.06194005161523819,
-0.031026380136609077,
-0.02012254111468792,
0.013239551335573196,
-0.029860395938158035,
0.010809730738401413,
0.02923988364636898,
0.05869819596409798,
-0.00652378611266613,
-0.0230573657900095,
-0.03179740533232689,
-0.053595952689647675,
0.0325419083237648,
-0.0020813990850001574,
-0.04417196661233902,
0.04149898141622543,
-0.04222165420651436,
0.03682861477136612,
0.039982762187719345
] |
PMID:25071 | Clinical experiments with the new oral long-acting neuroleptic clopimozide (R 29 764). | A pilot study with the new oral long-acting neuroleptic agent clopimozide, a diphenylbutylpiperidine derivative, has been reported. 30 female patients, only 20 of whom completed the trial, were treated with dosages of between 5 and 35 mg. The treatment comprised four periods in which the maximum tolerated daily dosage, the optimal weekly dosage (mean: 20.25 mg), the maximum tolerated weekly dosage and the optimal daily dosage were to be determined. In 10 patients plasma levels were determined with the radio-immunological method. The product exhibited a marked effect on schizophrenic target symptoms (significant at the 0.001 and 0.01 levels), of at least one week's duration, with an absence of sedation and a low degree of extrapyramidal side-effects. Clopimozide appears to be particularly suitable for the neuroleptic long-term treatment of compensated schizophrenic patients with insight into their disease. | Clinical experiments with the new oral long-acting neuroleptic clopimozide (R 29 764). A pilot study with the new oral long-acting neuroleptic agent clopimozide, a diphenylbutylpiperidine derivative, has been reported. 30 female patients, only 20 of whom completed the trial, were treated with dosages of between 5 and 35 mg. The treatment comprised four periods in which the maximum tolerated daily dosage, the optimal weekly dosage (mean: 20.25 mg), the maximum tolerated weekly dosage and the optimal daily dosage were to be determined. In 10 patients plasma levels were determined with the radio-immunological method. The product exhibited a marked effect on schizophrenic target symptoms (significant at the 0.001 and 0.01 levels), of at least one week's duration, with an absence of sedation and a low degree of extrapyramidal side-effects. Clopimozide appears to be particularly suitable for the neuroleptic long-term treatment of compensated schizophrenic patients with insight into their disease. | [
-0.08094987273216248,
0.03741161525249481,
0.02181936614215374,
0.03189956396818161,
-0.03984765335917473,
0.01635347679257393,
0.034290749579668045,
0.02658001147210598,
-0.056011494249105453,
-0.044712115079164505,
0.056575048714876175,
0.004961174912750721,
0.02471459098160267,
0.04731718823313713,
0.008065612986683846,
-0.008097735233604908,
0.04461213946342468,
-0.08235123008489609,
0.02377833053469658,
0.02461148612201214,
0.0072063272818923,
-0.0543818324804306,
0.05211377143859863,
0.02413639798760414,
0.0005261035985313356,
0.05670130252838135,
0.04472965747117996,
-0.03905857354402542,
-0.03647439926862717,
-0.17123286426067352,
0.00997653417289257,
0.04680364206433296,
-0.010204745456576347,
-0.05692824348807335,
-0.03628050163388252,
0.0034467149525880814,
-0.03687673807144165,
0.07174987345933914,
-0.007684075739234686,
0.03424636647105217,
0.06964702159166336,
0.06877520680427551,
-0.0002808395365718752,
-0.02767327055335045,
-0.021131649613380432,
-0.051709551364183426,
-0.007385718636214733,
-0.00069989706389606,
0.0896839052438736,
0.043905701488256454,
0.008319821208715439,
-0.015534093603491783,
0.04424438625574112,
0.024474509060382843,
0.026251696050167084,
-0.0006218082853592932,
-0.020076008513569832,
-0.005690048914402723,
-0.04816056042909622,
0.05502546206116676,
-0.03066210076212883,
-0.004058951046317816,
-0.14101865887641907,
0.06839118152856827,
-0.0011357637122273445,
0.01117450837045908,
-0.02079194411635399,
-0.05945354327559471,
0.016200926154851913,
0.05190974101424217,
-0.06475570052862167,
-0.00462097255513072,
0.003526831278577447,
0.06210337579250336,
0.0037139758933335543,
-0.008011134341359138,
0.013715974986553192,
-0.025728512555360794,
-0.031208151951432228,
-0.009621749632060528,
0.021047018468379974,
-0.027945589274168015,
-0.016896313056349754,
-0.06250911206007004,
0.03263041749596596,
-0.025034260004758835,
0.004840735346078873,
0.010729895904660225,
0.007687133736908436,
-0.001615410903468728,
0.031338900327682495,
-0.019408849999308586,
0.02254396118223667,
-0.000808034383226186,
-0.040395788848400116,
0.03953581303358078,
-0.013416912406682968,
-0.017981763929128647,
-0.00984411034733057,
0.4143598973751068,
0.009417231194674969,
-0.034188203513622284,
-0.05834191292524338,
-0.017912546172738075,
0.03668804466724396,
0.023673992604017258,
0.031807657331228256,
0.01943054422736168,
0.03920874372124672,
0.028951087966561317,
-0.016764378175139427,
0.052579332143068314,
0.09501629322767258,
-0.03956860676407814,
-0.08936569094657898,
0.011313364841043949,
0.04515905678272247,
-0.008710530586540699,
-0.021823078393936157,
0.04577493295073509,
-0.05706038326025009,
-0.018558278679847717,
0.030126109719276428,
-0.016814982518553734,
-0.01656847819685936,
-0.00001696517392701935,
-0.011923172511160374,
0.058242976665496826,
0.0067556751891970634,
0.013248691335320473,
0.046801116317510605,
-0.013460326939821243,
-0.013966559432446957,
0.06672953069210052,
0.04210739582777023,
0.020923972129821777,
0.0150159802287817,
-0.049767568707466125,
0.037659477442502975,
-0.032169826328754425,
0.04269193112850189,
-0.0505903884768486,
0.004972821567207575,
-0.08212779462337494,
-0.03723575919866562,
0.06864546984434128,
-0.008693059906363487,
0.03642378747463226,
-0.02969658374786377,
0.042271580547094345,
-0.016508564352989197,
0.09314298629760742,
0.004365385975688696,
0.0009240898070856929,
-0.02858094312250614,
0.0353749543428421,
0.04409444332122803,
-0.032177407294511795,
-0.07265949249267578,
0.02410852536559105,
-0.03617337718605995,
-0.04551518335938454,
0.027818134054541588,
0.05287336930632591,
0.011104168370366096,
-0.04358985275030136,
-0.01769404113292694,
-0.05350295454263687,
-0.06705237925052643,
0.04670408368110657,
-0.0016788674984127283,
-0.01037044171243906,
-0.004724195227026939,
0.020207876339554787,
-0.07966245710849762,
-0.015439275652170181,
0.015906361863017082,
0.02675825171172619,
-0.02643776126205921,
-0.016235163435339928,
-0.016629280522465706,
0.02230796217918396,
-0.02973533421754837,
-0.02673031948506832,
-0.030953876674175262,
0.005977259948849678,
0.00021062634186819196,
0.028964629396796227,
0.003823076141998172,
0.01515315379947424,
-0.007708507124334574,
0.017926760017871857,
-0.04505888372659683,
0.056913867592811584,
-0.006146327126771212,
-0.06242571026086807,
-0.09163063019514084,
0.0013488339027389884,
-0.026729766279459,
-0.02705552615225315,
0.06431838870048523,
0.04596902057528496,
0.015175440348684788,
0.00016567751299589872,
0.05951524153351784,
0.03841429948806763,
0.03711874783039093,
-0.023185377940535545,
0.05203135684132576,
-0.06551768630743027,
-0.022000445052981377,
0.002143306890502572,
0.07332152128219604,
0.05065055564045906,
-0.046138614416122437,
0.09083257615566254,
-0.04306618496775627,
-0.016252469271421432,
0.07208450138568878,
0.05519614741206169,
0.003816412528976798,
-0.0024448796175420284,
-0.028028225526213646,
-0.3108070492744446,
-0.04853954538702965,
0.024704670533537865,
-0.030633218586444855,
0.09162843227386475,
-0.0714409351348877,
0.0027976317796856165,
-0.07835497707128525,
0.024110060185194016,
0.005624336656183004,
0.001171760493889451,
0.005163329187780619,
-0.06364815682172775,
-0.04065847024321556,
-0.025137007236480713,
-0.0064087253995239735,
0.07411503046751022,
-0.04072465002536774,
-0.039332401007413864,
0.009006570093333721,
0.011455366387963295,
-0.051536910235881805,
-0.04317907989025116,
0.03355272859334946,
0.025031540542840958,
-0.02003963477909565,
0.08922254294157028,
0.004904606845229864,
-0.001286209444515407,
0.0154963918030262,
-0.012711289338767529,
0.011414580047130585,
-0.04715007171034813,
-0.10134720802307129,
-0.021481310948729515,
-0.04050083085894585,
-0.0087589668110013,
-0.04650932550430298,
-0.038218144327402115,
-0.004130783956497908,
-0.09597272425889969,
0.008233597502112389,
0.007747343275696039,
-0.0901053249835968,
-0.10043562203645706,
0.03432481735944748,
-0.0055577754974365234,
0.05775627866387367,
-0.003996582236140966,
-0.03696855530142784,
-0.01770828291773796,
0.0037985541857779026,
0.018383344635367393,
-0.022049592807888985,
0.02816673554480076,
-0.004142584279179573,
-0.07395592331886292,
-0.012696106918156147,
-0.0600285679101944,
-0.028763258829712868,
0.035188913345336914,
0.04330580309033394,
0.01906374841928482,
-0.013016453012824059,
-0.010015489533543587,
-0.030409932136535645,
0.041512973606586456,
-0.015450081788003445,
0.04648450389504433,
0.024527693167328835,
-0.0008950985502451658,
0.0994051992893219,
-0.03890737518668175,
0.05650762841105461,
0.07133451104164124,
0.037127625197172165,
0.013828570954501629,
0.007259646896272898,
-0.0358729213476181,
0.04121452942490578,
0.01467126701027155,
0.006246557924896479,
0.044356077909469604,
0.03964342176914215,
-0.004512276966124773,
-0.03677027300000191,
0.0048337881453335285,
-0.031035054475069046,
0.03537827730178833,
-0.01251163799315691,
0.02923509106040001,
-0.036219626665115356,
-0.05677195265889168,
-0.04364794120192528,
0.021872643381357193,
0.02064870111644268,
-0.2624932825565338,
0.04253337159752846,
-0.001824752427637577,
0.05987853556871414,
0.006251759361475706,
0.04343608021736145,
-0.002590365242213011,
0.02458076924085617,
-0.09240267425775528,
0.014697344973683357,
-0.023827282711863518,
0.031091773882508278,
0.04882602393627167,
0.029678085818886757,
-0.006513446103781462,
-0.022643957287073135,
0.051078736782073975,
0.022393250837922096,
0.04835875332355499,
0.007187425158917904,
0.023470353335142136,
-0.005712572950869799,
0.1073366180062294,
0.07705917954444885,
0.01616050861775875,
0.022518957033753395,
0.009946939535439014,
0.029747672379016876,
-0.06257177144289017,
-0.016506854444742203,
-0.040804438292980194,
-0.005343342665582895,
0.057254645973443985,
0.00016799723380245268,
-0.000562630477361381,
0.029458707198500633,
-0.056492745876312256,
-0.05583177134394646,
-0.010383781976997852,
0.008575507439672947,
-0.0019525802927091718,
0.018489817157387733,
0.014407657086849213,
0.032738346606492996,
0.08311472088098526,
-0.07615700364112854,
-0.05390215292572975,
-0.04061397165060043,
-0.011683479882776737,
0.042206887155771255,
-0.008064069785177708,
-0.05254252254962921,
0.044916871935129166,
0.04816272109746933,
0.025043034926056862,
0.005547550972551107,
0.014452514238655567,
-0.007630223874002695,
0.04214926064014435,
-0.05280635505914688,
-0.0712522491812706,
-0.004224350210279226,
-0.020578976720571518,
0.08355981111526489,
0.009204315952956676
] |
PMID:25075 | Beta-blockers and asthma. | In a single-blind, randomised, crossover study in 10 asthmatic patients, the effects of approximately equipotent oral doses of 3 cardioselective beta-blockers-atenolol (100 mg), metoprolol (100 mg), and acebutolol (300 mg)-and 4 non-cardioselective beta-blockers-proranolol (100 mg), oxprenolol (100 mg), pindolol (5 mg), and timolol (10 mg) upon FEV1 were compared. All drugs, except pindolol, produced a significant reduction in standing pulse rate and prevented an increase in heart rate after inhaled isoprenaline (1500 microgram). All drugs caused a fall in FEV1 but only atenolol did not differ significantly from placebo in this respect. The bronchodilator response to inhaled isoprenaline was blocked by the 4 non-cardioselective drugs; the 3 cardioselective agents permitted some bronchodilatation, but only atenolol did not differ from placebo. | Beta-blockers and asthma. In a single-blind, randomised, crossover study in 10 asthmatic patients, the effects of approximately equipotent oral doses of 3 cardioselective beta-blockers-atenolol (100 mg), metoprolol (100 mg), and acebutolol (300 mg)-and 4 non-cardioselective beta-blockers-proranolol (100 mg), oxprenolol (100 mg), pindolol (5 mg), and timolol (10 mg) upon FEV1 were compared. All drugs, except pindolol, produced a significant reduction in standing pulse rate and prevented an increase in heart rate after inhaled isoprenaline (1500 microgram). All drugs caused a fall in FEV1 but only atenolol did not differ significantly from placebo in this respect. The bronchodilator response to inhaled isoprenaline was blocked by the 4 non-cardioselective drugs; the 3 cardioselective agents permitted some bronchodilatation, but only atenolol did not differ from placebo. | [
-0.042571261525154114,
0.022120598703622818,
-0.0032450135331600904,
0.034436799585819244,
-0.01830109767615795,
-0.017993489280343056,
0.029603775590658188,
0.024290254339575768,
-0.05434585362672806,
-0.03939439728856087,
-0.02994561195373535,
-0.00799737311899662,
0.0002383143873885274,
0.03269397094845772,
0.002011181553825736,
0.03352568298578262,
0.06629456579685211,
-0.05303815007209778,
-0.10670791566371918,
0.014306961558759212,
-0.013077744282782078,
-0.0010708332993090153,
0.01870226301252842,
-0.008764766156673431,
0.028937416151165962,
0.04891207814216614,
-0.016905726864933968,
0.004755300469696522,
-0.014753344468772411,
-0.13952501118183136,
0.0016702937427908182,
0.015463277697563171,
0.04916948825120926,
0.0076445466838777065,
-0.050472602248191833,
0.02115487866103649,
-0.03966718539595604,
0.03476481884717941,
-0.030407870188355446,
0.04992767795920372,
0.013833170756697655,
0.0094293262809515,
-0.021913571283221245,
0.003407350042834878,
-0.04058469086885452,
0.004882745444774628,
-0.017209429293870926,
0.003922399133443832,
0.11172518134117126,
-0.02803027257323265,
0.06645853072404861,
0.004813869949430227,
-0.02984573505818844,
0.025868846103549004,
-0.04107467457652092,
-0.049973610788583755,
-0.06362341344356537,
0.02861095406115055,
0.0021104454062879086,
-0.004121286794543266,
-0.058929625898599625,
0.023801425471901894,
-0.14133478701114655,
0.10302289575338364,
0.020051319152116776,
0.04276008531451225,
-0.026017935946583748,
0.01516810990869999,
0.033660851418972015,
0.13137993216514587,
0.03320179879665375,
0.02007855288684368,
0.005093005485832691,
0.010500394739210606,
0.009965023025870323,
0.010667369700968266,
-0.005418881308287382,
-0.003874722169712186,
0.005587312392890453,
-0.027896234765648842,
0.027333131060004234,
0.02728927694261074,
-0.058995865285396576,
-0.030362790450453758,
0.005574496928602457,
-0.05463389679789543,
0.07678641378879547,
-0.006985546555370092,
-0.01708965376019478,
-0.009460913017392159,
-0.041613977402448654,
-0.02827892079949379,
0.020783545449376106,
0.025225909426808357,
0.038545846939086914,
-0.03292405605316162,
-0.026347797363996506,
-0.03729895502328873,
-0.05224765092134476,
0.36325547099113464,
0.01902013272047043,
0.07622405141592026,
-0.07592081278562546,
-0.025267766788601875,
0.03284339979290962,
-0.06054184213280678,
-0.016903935000300407,
-0.02887958474457264,
-0.0039621759206056595,
0.019148088991642,
0.05579162761569023,
-0.050528403371572495,
0.07495018094778061,
-0.050125204026699066,
-0.01521469559520483,
-0.02821785770356655,
0.07635404169559479,
-0.0496448315680027,
0.003595026908442378,
0.007315775379538536,
-0.020935524255037308,
-0.004391826223582029,
0.041773486882448196,
0.030089417472481728,
0.021664850413799286,
0.011296537704765797,
-0.001083027571439743,
0.12348884344100952,
0.03458492457866669,
0.02630804292857647,
0.07462280243635178,
0.030553149059414864,
-0.044335659593343735,
-0.01358246523886919,
0.03586240112781525,
-0.01644073985517025,
0.07753836363554001,
0.006383542437106371,
0.008717451244592667,
-0.006367777939885855,
0.02420911006629467,
-0.059237509965896606,
0.07614031434059143,
-0.11089354008436203,
-0.09579076617956161,
0.08032182604074478,
0.005520160775631666,
0.02800203301012516,
-0.04183128476142883,
0.038339994847774506,
-0.058318398892879486,
0.05078676715493202,
-0.04784856736660004,
0.05023360252380371,
-0.042123034596443176,
-0.019682960584759712,
0.038919687271118164,
0.04345878213644028,
-0.0011415292974561453,
-0.025005461648106575,
-0.09266264736652374,
-0.014818585477769375,
-0.02842981182038784,
0.017259465530514717,
-0.03924347460269928,
0.004502861760556698,
-0.08631931245326996,
0.008640894666314125,
-0.011908563785254955,
0.017954550683498383,
0.0000016584415334364166,
-0.03723008930683136,
0.03615104407072067,
-0.05507238581776619,
0.004892466124147177,
0.0049701863899827,
0.06159372627735138,
-0.049450576305389404,
0.05644194781780243,
-0.05134526640176773,
0.014601623639464378,
0.03308562934398651,
0.03321167081594467,
-0.022317586466670036,
-0.01728830114006996,
0.00520913302898407,
-0.04457612708210945,
-0.04515650495886803,
0.009088583290576935,
0.04227761551737785,
0.00892648845911026,
-0.024192679673433304,
-0.0879414975643158,
-0.014838737435638905,
-0.051324762403964996,
-0.05693390965461731,
-0.05991530790925026,
-0.01377803273499012,
-0.001574927824549377,
-0.005641104653477669,
0.07291372865438461,
0.004103120882064104,
0.01976996287703514,
0.0424441322684288,
0.01670883595943451,
0.06118931248784065,
-0.012663003988564014,
0.02225467748939991,
0.06720608472824097,
-0.031277015805244446,
0.03664387762546539,
0.03134876489639282,
0.043973833322525024,
0.06216629222035408,
-0.0490262471139431,
0.00921030342578888,
-0.03832859545946121,
-0.015993453562259674,
0.044329337775707245,
0.006460899021476507,
0.026868166401982307,
0.021233120933175087,
0.04813330993056297,
-0.30571138858795166,
-0.08510083705186844,
-0.023338811472058296,
-0.021123867481946945,
-0.007821510545909405,
-0.028386058285832405,
0.04291258752346039,
-0.031946007162332535,
-0.03314672037959099,
-0.04723484069108963,
0.011254868470132351,
0.03382475674152374,
0.007463877554982901,
0.03803566098213196,
-0.009093155153095722,
0.10371411591768265,
0.03258676454424858,
-0.04247000813484192,
0.013875257223844528,
0.02438485436141491,
-0.004735677968710661,
-0.058404166251420975,
-0.044467177242040634,
0.019537895917892456,
0.09916567802429199,
-0.018485622480511665,
0.06461481004953384,
-0.014016843400895596,
-0.05043957009911537,
0.0005381956580094993,
0.011927644722163677,
0.004573892802000046,
0.011402999050915241,
-0.037103600800037384,
-0.00002043672611762304,
-0.026604052633047104,
0.07255067676305771,
0.013595388270914555,
0.0007116417400538921,
-0.005296891089528799,
-0.02786276303231716,
-0.00754260178655386,
0.009186677634716034,
-0.020545896142721176,
-0.07728207856416702,
-0.02195010520517826,
-0.005702049937099218,
0.0770506039261818,
-0.025138361379504204,
0.03268533945083618,
0.0002893194032367319,
-0.0023898875806480646,
0.013568708673119545,
-0.02510247752070427,
0.07643385976552963,
-0.010791044682264328,
-0.05669661983847618,
-0.021664394065737724,
0.003455252619460225,
-0.0077561307698488235,
-0.022637708112597466,
-0.05963733047246933,
0.04474516212940216,
0.01316741295158863,
-0.03946186602115631,
0.00001882901597127784,
0.04792012646794319,
-0.008310499601066113,
0.0411025770008564,
0.0004702533478848636,
0.0031826177146285772,
0.1023746132850647,
-0.05413324758410454,
-0.025667473673820496,
0.06308649480342865,
0.004263436887413263,
0.01143128052353859,
-0.04330369830131531,
-0.05455812066793442,
0.007908894680440426,
-0.020469801500439644,
0.0025297582615166903,
0.016185786575078964,
0.007710922043770552,
-0.04175826534628868,
-0.025886796414852142,
-0.01851743645966053,
0.048935674130916595,
0.005276448093354702,
-0.015355851501226425,
-0.029412945732474327,
0.013424216769635677,
-0.020923634991049767,
0.013425997458398342,
0.02468521147966385,
0.036218542605638504,
-0.21825771033763885,
0.007628625724464655,
0.06737501174211502,
-0.004030571319162846,
0.012923686765134335,
-0.02954299934208393,
0.05329860746860504,
-0.018299130722880363,
-0.052787020802497864,
-0.0036286029499024153,
-0.033320169895887375,
0.0408606231212616,
0.08132947236299515,
0.06570997834205627,
-0.04611027240753174,
-0.0014777341857552528,
0.09221359342336655,
-0.013518448919057846,
0.06466452777385712,
-0.027711447328329086,
0.01002485305070877,
-0.03356516733765602,
0.14568430185317993,
0.014815377071499825,
-0.1042829304933548,
0.05156688019633293,
0.013833698816597462,
0.01598859578371048,
-0.07628457248210907,
0.05656759440898895,
-0.03793532773852348,
-0.013723643496632576,
0.08660446107387543,
-0.0060851383022964,
-0.019836440682411194,
0.0037805307656526566,
-0.06020975112915039,
-0.003146807663142681,
-0.005513530224561691,
0.02417292259633541,
-0.061037108302116394,
-0.00216510146856308,
-0.056592538952827454,
-0.0009938196744769812,
0.05821167305111885,
-0.08647125214338303,
-0.09535765647888184,
0.014737984165549278,
0.010644215159118176,
0.02311539649963379,
-0.014474160969257355,
-0.024143991991877556,
0.055798258632421494,
0.09255275130271912,
0.054441604763269424,
-0.005873128306120634,
-0.014582015573978424,
0.006424957420676947,
-0.08428902924060822,
0.010341066867113113,
-0.018552131950855255,
-0.013597211800515652,
-0.0572504922747612,
0.07700181752443314,
0.09778816252946854
] |
PMID:25076 | Comparison of diazepam and flunitrazepam for sedation during local anaesthesia for bronchoscopy. | Diazepam and flunitrazepam were compared as amnesic and sedative adjuncts to local anaesthesia for diagnostic bronchoscopy in 92 patients. After local anaesthesia of the pharynx, larynx and trachea with lignocaine, atropine plus diazepam of flunitrazepam was injected i.v. The co-operation of the patients and the technical circumstances under which the bronchoscopy was performed were good in each group. None of the treatments significantly modified arterial pressure or heart rate. Two hours after the injection, flunitrazepam 0.01 mg kg-1 more frequently caused amnesia for pictures shown to the patients during the first 15 min after injection (failure to recall 42--75%, and for bronchoscopy 67%), than did diazepam 0.125 mg kg-1 (failure to recall 21--67%; bronchoscopy 38%). Double doses of the drugs caused amnesic actions similar to those of flunitrazepam 0.01 mg kg-1. When failure to recall was assessed on the following day, 29% and 5% of the patients remembered bronchoscopy after flunitrazepam 0.01 and 0.02 mg kg-1 respectively; after diazepam 0.125 and 0.25 mg kg-1 the corresponding percentages was 59% and 30% (P less than 0.05% v. fluintrazepam). The ability to stand and walk on a stright line was similar after the smaller doses of both drugs, but after the larger doses recovery was slower after flunitrazepam. Psychomotor performance was still distinctly impaired 2 h after the injection of the larger doses. | Comparison of diazepam and flunitrazepam for sedation during local anaesthesia for bronchoscopy. Diazepam and flunitrazepam were compared as amnesic and sedative adjuncts to local anaesthesia for diagnostic bronchoscopy in 92 patients. After local anaesthesia of the pharynx, larynx and trachea with lignocaine, atropine plus diazepam of flunitrazepam was injected i.v. The co-operation of the patients and the technical circumstances under which the bronchoscopy was performed were good in each group. None of the treatments significantly modified arterial pressure or heart rate. Two hours after the injection, flunitrazepam 0.01 mg kg-1 more frequently caused amnesia for pictures shown to the patients during the first 15 min after injection (failure to recall 42--75%, and for bronchoscopy 67%), than did diazepam 0.125 mg kg-1 (failure to recall 21--67%; bronchoscopy 38%). Double doses of the drugs caused amnesic actions similar to those of flunitrazepam 0.01 mg kg-1. When failure to recall was assessed on the following day, 29% and 5% of the patients remembered bronchoscopy after flunitrazepam 0.01 and 0.02 mg kg-1 respectively; after diazepam 0.125 and 0.25 mg kg-1 the corresponding percentages was 59% and 30% (P less than 0.05% v. fluintrazepam). The ability to stand and walk on a stright line was similar after the smaller doses of both drugs, but after the larger doses recovery was slower after flunitrazepam. Psychomotor performance was still distinctly impaired 2 h after the injection of the larger doses. | [
0.018366245552897453,
-0.027315260842442513,
0.010825781151652336,
0.037763793021440506,
0.019975800067186356,
0.007203734014183283,
0.04576415196061134,
0.04174553230404854,
-0.017208626493811607,
-0.07841172814369202,
-0.05551059916615486,
-0.002282679546624422,
0.00753962155431509,
-0.0012796851806342602,
0.005782241001725197,
-0.036533694714307785,
0.020941251888871193,
-0.06076810136437416,
-0.05605841800570488,
0.02539793588221073,
-0.05826094001531601,
0.07299135625362396,
0.02943454124033451,
0.00790176447480917,
-0.005068609490990639,
-0.003457089653238654,
0.005945915821939707,
0.00724210636690259,
0.011420981958508492,
-0.15936242043972015,
-0.02335607260465622,
-0.0055528562515974045,
-0.00043436847045086324,
-0.03568042069673538,
-0.033775679767131805,
-0.019239109009504318,
-0.0073937540873885155,
0.04589943587779999,
-0.027940329164266586,
0.030241940170526505,
0.058435942977666855,
0.07650575786828995,
-0.05036857724189758,
-0.011876304633915424,
-0.054836031049489975,
-0.014343163929879665,
0.04030401259660721,
-0.045624177902936935,
0.13037030398845673,
0.012840637937188148,
0.03582935035228729,
-0.03867882862687111,
0.055099036544561386,
0.034303419291973114,
-0.04326360672712326,
-0.014741201885044575,
-0.04200925678014755,
0.0007894681766629219,
-0.04026235267519951,
-0.007662269752472639,
-0.003452862147241831,
0.07132524996995926,
-0.1485746204853058,
-0.0051188417710363865,
-0.004580836743116379,
-0.020972900092601776,
0.01845945604145527,
-0.062278009951114655,
0.03989233821630478,
0.0714588314294815,
-0.030861658975481987,
-0.014032213017344475,
-0.03563189506530762,
0.033744096755981445,
0.0029035985935479403,
-0.015659108757972717,
0.057417795062065125,
0.03271547332406044,
-0.04504500702023506,
-0.04640974476933479,
0.04000229015946388,
-0.003189002862200141,
0.0014507633168250322,
-0.07950794696807861,
0.029586000367999077,
-0.01968623697757721,
0.053525906056165695,
0.009687215089797974,
-0.0032596937380731106,
0.01789374276995659,
0.05945196747779846,
0.0022490781266242266,
0.008880440145730972,
-0.04598388820886612,
-0.0047592963092029095,
0.020341623574495316,
0.004673646297305822,
0.07808753103017807,
-0.02844228222966194,
0.360980361700058,
0.04803355410695076,
0.017210587859153748,
-0.11245089769363403,
-0.03422601521015167,
0.0004362888867035508,
-0.058906517922878265,
0.02292150817811489,
-0.022323153913021088,
-0.005743354558944702,
0.04336386173963547,
0.053546883165836334,
-0.03323528170585632,
0.10682249814271927,
0.029836786910891533,
-0.0584920309484005,
0.040224961936473846,
0.10437868535518646,
0.062369439750909805,
0.02022937498986721,
0.015904709696769714,
0.010790086351335049,
-0.007561666890978813,
0.02048150636255741,
-0.018023483455181122,
0.04144403338432312,
-0.03417075052857399,
-0.014009128324687481,
0.0765942931175232,
0.014498265460133553,
-0.01574089005589485,
0.04480096325278282,
0.00010061760258395225,
0.049558646976947784,
-0.004163092002272606,
0.0035571223124861717,
-0.0012139743193984032,
0.05028318241238594,
-0.050340667366981506,
0.03682030737400055,
-0.03526065871119499,
0.026418443769216537,
-0.09014051407575607,
0.006374097894877195,
-0.04676109179854393,
-0.09027504175901413,
0.0843549519777298,
-0.07576868683099747,
-0.03581203147768974,
-0.046422798186540604,
-0.03371211886405945,
-0.02184780314564705,
-0.020956089720129967,
-0.04438916593790054,
0.06701593101024628,
-0.008584662340581417,
0.047513168305158615,
0.023547185584902763,
0.07039522379636765,
-0.01520164031535387,
0.0268831979483366,
-0.04063011333346367,
-0.032411642372608185,
-0.000984081532806158,
0.055270008742809296,
-0.017903584986925125,
0.017930258065462112,
-0.025764523074030876,
-0.022645819932222366,
-0.021147174760699272,
0.03630859777331352,
0.0028430402744561434,
-0.019281113520264626,
-0.01936614327132702,
-0.05404021590948105,
-0.03136841580271721,
-0.002246879506856203,
-0.01050487905740738,
-0.01647539809346199,
0.034664835780858994,
-0.04247406870126724,
0.026058515533804893,
0.02536124549806118,
-0.0011829589493572712,
-0.01302785612642765,
0.016881657764315605,
-0.002002446446567774,
0.020112676545977592,
-0.012054982595145702,
0.02076280675828457,
0.026102222502231598,
-0.02511056512594223,
0.002736375667154789,
-0.06735009700059891,
-0.027079209685325623,
0.01582506112754345,
-0.042112577706575394,
-0.03745820373296738,
0.02647957019507885,
0.018710127100348473,
-0.023171307519078255,
0.10781446844339371,
0.07877855747938156,
-0.009335692040622234,
0.0456446148455143,
0.004511612001806498,
0.03158194571733475,
-0.04268176481127739,
0.03801226243376732,
0.04062342271208763,
-0.02060370333492756,
0.0019050855189561844,
0.02506856434047222,
0.05149582028388977,
0.010490160435438156,
-0.02628222480416298,
-0.002609401475638151,
-0.05032104626297951,
0.031237028539180756,
-0.025785529986023903,
0.009166060015559196,
0.02531556598842144,
-0.006824921350926161,
0.05577043071389198,
-0.31533995270729065,
-0.017203882336616516,
0.04654872044920921,
0.016556616872549057,
0.0918511152267456,
0.009332671761512756,
0.09181814640760422,
-0.020877307280898094,
-0.055888548493385315,
-0.04483127221465111,
-0.05528432875871658,
-0.044089604169130325,
-0.04650193825364113,
0.02798091247677803,
0.00395886180922389,
0.06710755079984665,
0.0448266975581646,
-0.0490572527050972,
0.025653153657913208,
-0.03670607507228851,
-0.05518754571676254,
-0.02281765453517437,
-0.05309625715017319,
0.0004225041775498539,
0.0174558125436306,
-0.020375458523631096,
0.09277831763029099,
-0.06915925443172455,
0.006616457365453243,
-0.029879087582230568,
0.0470278337597847,
0.021684039384126663,
0.0018394130747765303,
-0.0591735877096653,
0.019238460808992386,
0.00542265921831131,
0.014362605288624763,
0.030942851677536964,
-0.0024548047222197056,
0.007200018968433142,
-0.07441000640392303,
-0.01000234019011259,
0.061457376927137375,
-0.04654769226908684,
-0.04746371880173683,
-0.033375032246112823,
-0.04814236983656883,
-0.05515537038445473,
-0.08046973496675491,
-0.026622740551829338,
0.018605591729283333,
0.036784205585718155,
-0.02652718685567379,
-0.03843360021710396,
0.04892957955598831,
-0.017718788236379623,
-0.0573861338198185,
-0.02293955720961094,
-0.042898233979940414,
-0.04045230895280838,
0.006332043558359146,
0.01224582176655531,
0.0612686462700367,
-0.06451534479856491,
-0.014385897666215897,
0.026339953765273094,
0.03850333020091057,
0.0025329815689474344,
0.07431456446647644,
0.027206121012568474,
-0.010075496509671211,
0.10683495551347733,
-0.04616813361644745,
-0.08533144742250443,
0.06545606255531311,
0.03837801143527031,
0.03198491409420967,
-0.011125551536679268,
-0.09039867669343948,
0.024702241644263268,
-0.014376276172697544,
0.014257876202464104,
0.06716189533472061,
0.052119988948106766,
-0.04046601429581642,
-0.026467641815543175,
0.04025730863213539,
-0.013230259530246258,
-0.014634894207119942,
0.017066799104213715,
0.0027230214327573776,
0.00014869027654640377,
-0.03478645533323288,
0.014878306537866592,
-0.02191421017050743,
0.01076484378427267,
-0.26953670382499695,
-0.0023372529540210962,
0.0014587852638214827,
0.06451465934515,
0.012784657068550587,
-0.020707305520772934,
0.0388418547809124,
-0.011117245070636272,
-0.018750905990600586,
-0.03666246682405472,
0.0028528051916509867,
-0.03099801577627659,
0.05073418468236923,
0.037237923592329025,
-0.03598729148507118,
0.036354437470436096,
0.04822404682636261,
-0.005432263948023319,
0.05475936457514763,
-0.034819815307855606,
0.013810357078909874,
0.034141477197408676,
0.10330399870872498,
0.0036101057194173336,
-0.022827617824077606,
0.05718673765659332,
0.04182950034737587,
0.04188564419746399,
0.06462062150239944,
0.06166285276412964,
0.00475528696551919,
-0.014556635171175003,
0.04685376212000847,
-0.011007536202669144,
0.03111884742975235,
-0.006689103320240974,
-0.10982584208250046,
0.05408687889575958,
-0.020410126075148582,
0.0073645999655127525,
-0.012390396557748318,
0.010589977726340294,
-0.04047062620520592,
-0.03468090295791626,
0.06064453348517418,
-0.08338826894760132,
0.00948164239525795,
-0.07171174138784409,
-0.041837193071842194,
0.002363085513934493,
-0.006224494893103838,
-0.03289913013577461,
0.05063506215810776,
0.028918426483869553,
0.08598051965236664,
0.03354254737496376,
-0.009438082575798035,
-0.03627645596861839,
0.0039260052144527435,
0.029702922329306602,
-0.06207577511668205,
-0.04086194559931755,
-0.027688918635249138,
0.07572723925113678,
0.00045783803216181695
] |
PMID:25077 | Fazadinium in anaesthesia. | The clinical use of the neuromuscular blocking agent fazadinium was investigated in a survey of 500 patients. Rapid conditions for tracheal intubation followed by excellent relaxation and easy antagonism were obtained. It is suggested that these findings commend it as an alternative to suxamethonium. Tachycardia appeared to be the only disadvantage associated with its use. | Fazadinium in anaesthesia. The clinical use of the neuromuscular blocking agent fazadinium was investigated in a survey of 500 patients. Rapid conditions for tracheal intubation followed by excellent relaxation and easy antagonism were obtained. It is suggested that these findings commend it as an alternative to suxamethonium. Tachycardia appeared to be the only disadvantage associated with its use. | [
-0.003330493811517954,
0.02056499756872654,
0.006090966053307056,
0.04792174696922302,
-0.012309256941080093,
0.039212387055158615,
0.04887858033180237,
0.061153437942266464,
-0.021022453904151917,
-0.026931753382086754,
-0.03191620483994484,
-0.036955688148736954,
0.00826998520642519,
0.09311352670192719,
-0.008919734507799149,
-0.058748774230480194,
0.015787223353981972,
-0.014467686414718628,
0.011238647624850273,
-0.009500812739133835,
-0.05702272802591324,
0.07070805877447128,
-0.052804674953222275,
-0.07166724652051926,
-0.006824912037700415,
0.019739041104912758,
-0.024207070469856262,
0.012728226371109486,
-0.014429866336286068,
-0.13875800371170044,
0.015524027869105339,
-0.015813903883099556,
0.010888086631894112,
-0.025795424357056618,
-0.046520572155714035,
0.04054618999361992,
-0.06575150042772293,
0.019071554765105247,
-0.04973205178976059,
0.05659671500325203,
0.035079311579465866,
0.07687795162200928,
-0.013846198096871376,
0.009754515253007412,
-0.038041967898607254,
-0.04255540296435356,
0.015115099027752876,
-0.03279588371515274,
0.10949507355690002,
0.06547390669584274,
0.021080289036035538,
-0.0047204624861478806,
0.015180561691522598,
0.04878048598766327,
-0.021958814933896065,
-0.051111239939928055,
-0.0295578520745039,
0.008710931986570358,
-0.01911150850355625,
0.03667057305574417,
-0.02141891047358513,
0.04562974348664284,
-0.11487304419279099,
0.04332567751407623,
0.027880825102329254,
-0.02578287199139595,
0.028084423393011093,
-0.032804470509290695,
0.064779132604599,
0.07561925053596497,
-0.04492715001106262,
-0.02165960520505905,
-0.010160590521991253,
0.03307120129466057,
-0.009427795186638832,
-0.014996667392551899,
0.04591429606080055,
0.017652172595262527,
-0.024547029286623,
0.008519235998392105,
-0.02841702289879322,
-0.08471061289310455,
-0.03846680372953415,
-0.10600828379392624,
0.008280782960355282,
0.018047010526061058,
0.05418596789240837,
0.004560645669698715,
-0.013059999793767929,
-0.01990831084549427,
0.004127690568566322,
0.008546736091375351,
0.06723218411207199,
-0.012181474827229977,
-0.009921004064381123,
0.019477488473057747,
-0.047251421958208084,
0.035763803869485855,
-0.0717499852180481,
0.340585321187973,
-0.010178780183196068,
0.011431659571826458,
-0.04590398073196411,
-0.05689573660492897,
-0.024248357862234116,
-0.057864829897880554,
-0.010041892528533936,
-0.01476806029677391,
-0.01761952042579651,
0.052180733531713486,
0.05093091353774071,
0.000983993522822857,
0.07322162389755249,
-0.033776912838220596,
-0.07621413469314575,
0.03736129403114319,
0.08362284302711487,
-0.013916220515966415,
0.009620175696909428,
0.017623841762542725,
-0.013189963065087795,
-0.06173719838261604,
0.051337193697690964,
-0.053539928048849106,
0.05051128938794136,
-0.021588632836937904,
-0.07467679679393768,
0.06594767421483994,
0.02346721477806568,
0.017159665003418922,
0.09648354351520538,
-0.043066442012786865,
0.007223788648843765,
0.041808344423770905,
0.047789208590984344,
-0.01978732831776142,
0.09286569803953171,
-0.0006445570033974946,
-0.0015865545719861984,
-0.05042547732591629,
0.0013868148671463132,
-0.07910013198852539,
-0.00821519922465086,
-0.07434307038784027,
-0.09223595261573792,
0.07381652295589447,
-0.015973767265677452,
-0.006732364650815725,
-0.07628703117370605,
-0.00009523874177830294,
-0.023322321474552155,
0.06775065511465073,
-0.03116726689040661,
0.017566408962011337,
-0.02601156383752823,
0.009659585542976856,
0.010660854168236256,
0.08831837028265,
0.030440645292401314,
0.007970144972205162,
-0.06045960262417793,
-0.031177561730146408,
0.031370602548122406,
0.09673184901475906,
-0.018753347918391228,
-0.049128249287605286,
-0.051965538412332535,
0.03740460425615311,
-0.038541898131370544,
-0.02724231220781803,
-0.00784931518137455,
-0.006491616368293762,
0.0599936880171299,
-0.0023320293985307217,
-0.03179343417286873,
-0.0012423965381458402,
0.005649840924888849,
-0.09131043404340744,
0.02661352977156639,
0.006131654605269432,
0.0487227700650692,
0.03599557280540466,
-0.033417776226997375,
-0.05969591811299324,
0.032674167305231094,
0.01689540222287178,
-0.007417720276862383,
0.014726166613399982,
0.005559759680181742,
0.009386188350617886,
-0.019776392728090286,
0.011884081177413464,
-0.06129639595746994,
0.06655142456293106,
-0.02809310145676136,
-0.05835966393351555,
-0.015125080943107605,
0.04451385885477066,
-0.04380371421575546,
-0.04542958736419678,
0.0393405519425869,
0.031921036541461945,
0.003522761631757021,
0.06227262318134308,
0.04771742969751358,
0.03296559303998947,
0.030600788071751595,
-0.04274541139602661,
0.07929420471191406,
0.006956696044653654,
-0.06800669431686401,
0.060340262949466705,
0.033081602305173874,
0.008388365618884563,
-0.06737034022808075,
0.026842093095183372,
0.0027654310688376427,
-0.03260358050465584,
0.041926655918359756,
-0.00519634410738945,
0.09833116084337234,
0.006182394456118345,
0.020373525097966194,
-0.29011619091033936,
0.0038070515729486942,
-0.028626741841435432,
0.026075124740600586,
0.020726222544908524,
-0.032175078988075256,
0.05172593146562576,
-0.04525475576519966,
-0.09223804622888565,
-0.07556106895208359,
-0.037518955767154694,
0.023409824818372726,
-0.065218985080719,
-0.006052100565284491,
-0.01769709773361683,
0.03723987936973572,
0.031116826459765434,
-0.0002791392325889319,
0.021560806781053543,
0.003358934773132205,
0.04236546903848648,
0.03250998631119728,
-0.042825885117053986,
-0.008361687883734703,
0.06276816874742508,
0.006066957954317331,
0.09939248114824295,
-0.03202346712350845,
0.00902993232011795,
-0.04383079707622528,
0.00475355377420783,
0.028812652453780174,
-0.04819503426551819,
-0.06906796246767044,
-0.03571091219782829,
-0.03480977565050125,
0.056845519691705704,
0.004701385274529457,
0.03880942985415459,
-0.05558467283844948,
-0.07548950612545013,
-0.012285584583878517,
0.005035591311752796,
-0.010787720791995525,
-0.06099042668938637,
-0.018285667523741722,
-0.00460636243224144,
-0.04574771970510483,
-0.007424542680382729,
-0.036084745079278946,
0.03187244012951851,
0.05861357972025871,
-0.04616353288292885,
0.05881551280617714,
-0.062484391033649445,
-0.0010512410663068295,
-0.027646126225590706,
-0.017127200961112976,
0.01187373697757721,
-0.010943369939923286,
0.02565133571624756,
0.06397123634815216,
0.06765776872634888,
-0.01557848323136568,
-0.0429239422082901,
0.008325332775712013,
0.05164100602269173,
0.049103032797575,
0.064067043364048,
-0.028013911098241806,
-0.039666809141635895,
0.1389973759651184,
-0.04260284826159477,
0.028806006535887718,
0.049744147807359695,
0.014742099680006504,
0.020685279741883278,
0.02461596578359604,
-0.0348404236137867,
0.00306833372451365,
0.01135231927037239,
0.051054954528808594,
0.034454166889190674,
-0.046047843992710114,
-0.09030259400606155,
0.002014229306951165,
-0.018850907683372498,
-0.02062506414949894,
0.034343693405389786,
-0.034564364701509476,
-0.035025883466005325,
0.02867407165467739,
-0.06280069053173065,
0.027787568047642708,
0.027610918506979942,
-0.006950804498046637,
-0.2431831657886505,
-0.027259260416030884,
0.06845638155937195,
0.005280620884150267,
-0.019144229590892792,
-0.05594779551029205,
-0.028273362666368484,
0.012412656098604202,
-0.11282731592655182,
0.007373481057584286,
-0.03413349762558937,
0.03608116880059242,
0.03730892762541771,
0.0054066916927695274,
-0.007719916291534901,
0.027530118823051453,
0.054492074996232986,
-0.011514075100421906,
0.07244282960891724,
0.005203962791711092,
0.014109696261584759,
0.011296344920992851,
0.1388421207666397,
0.021992985159158707,
0.046127934008836746,
0.08364205062389374,
-0.017767636105418205,
-0.007244916167110205,
-0.015718065202236176,
0.03728723153471947,
0.0023551681078970432,
-0.017025228589773178,
0.033684249967336655,
-0.028056679293513298,
0.047739177942276,
-0.033201951533555984,
-0.036625202745199203,
0.04513822868466377,
-0.03626968339085579,
-0.013343940488994122,
-0.036069661378860474,
0.06188151612877846,
-0.006457595154643059,
0.021554259583353996,
0.07050605863332748,
-0.024262957274913788,
0.0003031330415979028,
-0.06182503700256348,
-0.013455822132527828,
0.07366155833005905,
-0.02094300463795662,
-0.061688683927059174,
0.01843090169131756,
0.02689562365412712,
0.07413868606090546,
-0.039958592504262924,
-0.006865169387310743,
-0.0076175881549716,
0.05042851343750954,
-0.008052407763898373,
-0.009166273288428783,
-0.015350421890616417,
-0.06000500172376633,
0.08167387545108795,
0.024151472374796867
] |
PMID:25078 | Acid hydrolases in blister fluid. I. Characterization and quantification of acid phosphatase. | Acid phosphatase has been characterized and quantified in human suction blister fluid, interstitial fluid and serum. The acid phosphatases of suction blister fluid and serum showed differences in their pH activity curves, Michaelis-Menton constants, heat stabilities and sensitivities to inhibition by tartrate and fluoride. The behaviour of the interstitial fluid enzyme was intermediate between these two. The levels of activity in blister fluid and serum were very similar, both being more than twice that of interstitial fluid. These results suggest that the acid phosphatase activity in suction blister fluid is derived largely from the overlying epidermis rather than from serum. | Acid hydrolases in blister fluid. I. Characterization and quantification of acid phosphatase. Acid phosphatase has been characterized and quantified in human suction blister fluid, interstitial fluid and serum. The acid phosphatases of suction blister fluid and serum showed differences in their pH activity curves, Michaelis-Menton constants, heat stabilities and sensitivities to inhibition by tartrate and fluoride. The behaviour of the interstitial fluid enzyme was intermediate between these two. The levels of activity in blister fluid and serum were very similar, both being more than twice that of interstitial fluid. These results suggest that the acid phosphatase activity in suction blister fluid is derived largely from the overlying epidermis rather than from serum. | [
-0.03037242405116558,
0.0007562231039628386,
-0.01895948126912117,
0.05993819609284401,
0.007432267069816589,
-0.0782037004828453,
0.09931980818510056,
0.051113955676555634,
-0.009181966073811054,
-0.06098426878452301,
0.005857429467141628,
-0.14289577305316925,
-0.011492341756820679,
0.004243812058120966,
-0.03743034601211548,
-0.022329585626721382,
0.060465775430202484,
0.012164423242211342,
-0.06001276150345802,
0.0440969318151474,
0.02279890701174736,
0.06375112384557724,
0.008235667832195759,
-0.07608607411384583,
-0.005082165356725454,
0.03677823767066002,
-0.005307422485202551,
0.02146168425679207,
-0.048888880759477615,
-0.1562972068786621,
0.022094041109085083,
-0.0670747384428978,
0.002303757006302476,
-0.04984008148312569,
-0.02024129405617714,
-0.012029563076794147,
-0.013650353066623211,
0.010951629839837551,
0.03914031386375427,
0.014882032759487629,
0.0010641305707395077,
-0.013016813434660435,
-0.01165310200303793,
0.07295752316713333,
0.032384783029556274,
-0.021096954122185707,
-0.04993920773267746,
0.036776091903448105,
0.0803527757525444,
-0.007951153442263603,
0.022338205948472023,
-0.01903153769671917,
0.023284537717700005,
0.042472850531339645,
-0.022632915526628494,
-0.037663619965314865,
-0.01721671223640442,
0.02065066248178482,
0.009019880555570126,
-0.02615404687821865,
0.029966821894049644,
0.0045948452316224575,
-0.11956831067800522,
0.046799398958683014,
0.0562114492058754,
0.016263408586382866,
0.011768726631999016,
-0.05148584023118019,
0.034253135323524475,
0.03267393261194229,
-0.060051724314689636,
0.06471864879131317,
0.0245048850774765,
0.02097499556839466,
-0.016361329704523087,
-0.03224203363060951,
-0.018995054066181183,
0.03326563164591789,
-0.03314078971743584,
0.0384613573551178,
-0.03816284239292145,
-0.02375827170908451,
0.04283265396952629,
0.028627371415495872,
0.012240082025527954,
0.012831012718379498,
0.05744007229804993,
0.018131345510482788,
0.024640483781695366,
0.03668256849050522,
-0.012215429916977882,
-0.055903833359479904,
-0.01586298458278179,
0.03576331213116646,
-0.0136973075568676,
0.04170786961913109,
0.034665413200855255,
0.01883082091808319,
0.004010466393083334,
0.3609431982040405,
-0.05550771206617355,
-0.021910497918725014,
-0.1090329959988594,
-0.05972912907600403,
-0.01476252730935812,
-0.004189948085695505,
-0.029247164726257324,
-0.026129597797989845,
-0.049140315502882004,
0.08523987978696823,
0.03431471064686775,
0.011868906207382679,
0.02226557768881321,
-0.10767005383968353,
-0.025976356118917465,
-0.038601167500019073,
0.03726497292518616,
0.05055758357048035,
0.026254354044795036,
0.040823888033628464,
0.0005726710660383105,
-0.02530534751713276,
0.012802877463400364,
-0.04825771972537041,
-0.0019962152000516653,
0.0501520037651062,
0.0648641437292099,
0.07478715479373932,
0.005684439092874527,
0.0007402798510156572,
0.0015019236598163843,
-0.03208247572183609,
-0.02757561206817627,
0.017094766721129417,
0.008158714510500431,
-0.025386173278093338,
0.03883794695138931,
0.015076338313519955,
0.050585050135850906,
0.05144491791725159,
-0.03619753196835518,
-0.038531817495822906,
-0.027875564992427826,
-0.0735020786523819,
0.02024279348552227,
0.11150171607732773,
-0.04687730595469475,
0.03097425401210785,
0.02244178205728531,
-0.006687337998300791,
0.020594444125890732,
0.027744542807340622,
-0.0483931265771389,
0.052783239632844925,
-0.01565014198422432,
-0.03645718842744827,
0.010683439671993256,
0.011424034833908081,
-0.0153318140655756,
-0.07306858897209167,
-0.026366254314780235,
-0.03533507511019707,
-0.009379832074046135,
0.053558122366666794,
0.0005821500672027469,
-0.016080088913440704,
0.047188546508550644,
-0.014981451444327831,
-0.0324903167784214,
-0.020735304802656174,
0.00649261474609375,
0.03259419649839401,
-0.028757136315107346,
-0.0388265922665596,
-0.030007673427462578,
0.013373542577028275,
0.03176717832684517,
0.053073566406965256,
0.0010699076810851693,
0.006128712557256222,
0.010355648584663868,
-0.00211810483597219,
-0.0771227777004242,
0.009750209748744965,
0.002387362765148282,
-0.07864520698785782,
0.015578880906105042,
0.029142992570996284,
0.005310554523020983,
0.04246716573834419,
-0.013443713076412678,
-0.06133114546537399,
-0.0551421120762825,
-0.0058242822997272015,
0.042658861726522446,
-0.007975051179528236,
-0.00007025343074928969,
0.010528014972805977,
-0.04259584844112396,
0.0082019018009305,
0.07217324525117874,
0.06291349977254868,
0.0024615926668047905,
0.05743606388568878,
0.0014838603092357516,
0.10374603420495987,
-0.06306489557027817,
0.02136741392314434,
0.04476214572787285,
0.010216077789664268,
-0.010451627895236015,
-0.0186188705265522,
-0.0966591164469719,
0.011542915366590023,
-0.05185079574584961,
0.005565471947193146,
0.043423086404800415,
-0.023301521316170692,
0.021909406408667564,
-0.02434103935956955,
0.03374338522553444,
0.014969853684306145,
-0.10025367140769958,
-0.2735345959663391,
-0.07272110134363174,
0.024776039645075798,
-0.01997321844100952,
0.05309537425637245,
-0.008593402802944183,
0.029585329815745354,
-0.014585377648472786,
-0.008925408124923706,
-0.10133880376815796,
0.05499906465411186,
0.008250918239355087,
-0.01063859649002552,
0.023252500221133232,
-0.0002652724797371775,
0.012561848387122154,
0.055302709341049194,
-0.028017813339829445,
0.06978535652160645,
0.006782623007893562,
0.021869108080863953,
-0.01727248728275299,
0.052815791219472885,
0.037153176963329315,
0.06417471915483475,
-0.014527834951877594,
0.099106565117836,
-0.026513351127505302,
-0.028125733137130737,
-0.039692409336566925,
0.05351904779672623,
0.060913991183042526,
-0.006706304848194122,
-0.08712116628885269,
-0.06919224560260773,
-0.03291437774896622,
0.007755729369819164,
-0.07693072408437729,
-0.03189939633011818,
-0.012575573287904263,
-0.06151521950960159,
0.010449711233377457,
-0.019491761922836304,
-0.0012932263780385256,
-0.019838187843561172,
0.004629296716302633,
0.04446137323975563,
0.023323917761445045,
-0.05230798199772835,
-0.03788140043616295,
0.05419163778424263,
-0.04200217127799988,
0.030656257644295692,
-0.051986467093229294,
-0.03982570767402649,
-0.017662139609456062,
0.01146756298840046,
-0.008981272578239441,
-0.07644634693861008,
-0.011575786396861076,
-0.0650758221745491,
0.018470417708158493,
0.014201839454472065,
0.057246509939432144,
0.01902303472161293,
-0.004395801108330488,
0.012142290361225605,
0.004136211238801479,
0.05306193232536316,
-0.03943053260445595,
-0.02798258140683174,
0.10842828452587128,
-0.03857337310910225,
-0.022334711626172066,
0.04541151970624924,
-0.006168443243950605,
0.020925376564264297,
-0.049740396440029144,
-0.047288212925195694,
-0.0000473876170872245,
0.047725096344947815,
-0.05539330095052719,
0.06956718862056732,
-0.0665254145860672,
0.007664056960493326,
0.06389342248439789,
0.042291004210710526,
-0.029285499826073647,
-0.007340537384152412,
0.031742341816425323,
0.03364984318614006,
0.01253136433660984,
-0.05969206616282463,
-0.012403681874275208,
0.030662767589092255,
-0.011531340889632702,
-0.28503018617630005,
0.01192512921988964,
0.05581651255488396,
-0.0006507810321636498,
-0.04358012229204178,
-0.01191327627748251,
0.07888416945934296,
0.009273067116737366,
0.050410427153110504,
0.01852078177034855,
0.01601286046206951,
0.051234327256679535,
0.04860077425837517,
0.04406838119029999,
-0.02072990871965885,
-0.0035212284419685602,
0.0728924497961998,
-0.02322082780301571,
0.03169028460979462,
0.0038069412112236023,
0.002742006676271558,
-0.04807928949594498,
0.16865652799606323,
0.006130926311016083,
0.05472478270530701,
0.08257578313350677,
0.000843919173348695,
0.010663126595318317,
-0.024952927604317665,
-0.011602465063333511,
0.0146277891471982,
0.016682470217347145,
0.04557054117321968,
0.02194528840482235,
-0.021859444677829742,
0.05446969345211983,
-0.0011810699943453074,
0.0014084693975746632,
0.046917349100112915,
0.011860515922307968,
0.06656621396541595,
-0.04458247497677803,
-0.07046567648649216,
-0.03543130308389664,
0.026118846610188484,
-0.07663288712501526,
-0.04564150050282478,
-0.03259206935763359,
-0.015005300752818584,
-0.035006362944841385,
-0.039222393184900284,
-0.08314499258995056,
0.029207773506641388,
0.057961221784353256,
-0.012311998754739761,
0.07628468424081802,
-0.046455685049295425,
-0.05371970310807228,
-0.007299777586013079,
0.009587189182639122,
0.020175296813249588,
-0.010079306550323963,
-0.04002217948436737,
0.03502245992422104,
0.019102033227682114
] |
PMID:25079 | Acid hydrolases in blister fluid. II. Characterization and quantification of glycoside hydrolases. | Seven glycoside hydrolases have been investigated in suction blister fluid, interstitial fluid and in serum. Six of these have been characterized; no differences could be demonstrated between the corresponding enzymes from the various sources. The remaining enzyme (beta-glucosidase) was not found. Quantitative data suggest that 2 enzymes (beta-acetylglucosaminidase and beta-glucuronidase) diffuse freely from the epidermis into blister fluid, whereas 4 (alpha-glucosidase, alpha- and beta-galactosidase and alpha-mannosidase) are almost entirely retained in the roof of the bulla. | Acid hydrolases in blister fluid. II. Characterization and quantification of glycoside hydrolases. Seven glycoside hydrolases have been investigated in suction blister fluid, interstitial fluid and in serum. Six of these have been characterized; no differences could be demonstrated between the corresponding enzymes from the various sources. The remaining enzyme (beta-glucosidase) was not found. Quantitative data suggest that 2 enzymes (beta-acetylglucosaminidase and beta-glucuronidase) diffuse freely from the epidermis into blister fluid, whereas 4 (alpha-glucosidase, alpha- and beta-galactosidase and alpha-mannosidase) are almost entirely retained in the roof of the bulla. | [
-0.01642470993101597,
-0.055322643369436264,
-0.027630526572465897,
0.05626465007662773,
0.026703668758273125,
-0.06675910204648972,
0.030426157638430595,
0.04172153398394585,
0.001831540954299271,
-0.07961598038673401,
-0.008805593475699425,
-0.1025189757347107,
-0.07789302617311478,
0.004602889996021986,
-0.026215076446533203,
-0.04171063378453255,
0.06255052983760834,
0.018941747024655342,
-0.05832470953464508,
-0.005327222868800163,
0.050159528851509094,
-0.003135118167847395,
0.0070226783864200115,
-0.0839061588048935,
-0.007311009336262941,
-0.016302628442645073,
-0.03310101479291916,
0.0051560960710048676,
-0.060699712485075,
-0.15012934803962708,
0.01511693187057972,
-0.06756636500358582,
-0.0008301129564642906,
-0.009499647654592991,
-0.020341427996754646,
0.01022419985383749,
0.0020223846659064293,
0.017577432096004486,
0.08605445176362991,
0.02801896072924137,
0.004613860510289669,
-0.001368251396343112,
-0.06601500511169434,
0.07093607634305954,
0.0035540228709578514,
-0.02518576942384243,
-0.06579796224832535,
0.012638379819691181,
0.09452249109745026,
0.005125556606799364,
-0.020879006013274193,
-0.0040266006253659725,
-0.0004469822742976248,
0.04724220559000969,
-0.034845635294914246,
-0.02291480079293251,
-0.007487270049750805,
-0.003334543900564313,
-0.001174266217276454,
0.018448377028107643,
0.039367228746414185,
0.004605039022862911,
-0.11457780748605728,
0.05281943082809448,
0.0695577934384346,
0.029982872307300568,
0.005377760156989098,
-0.017732596024870872,
0.03136618435382843,
0.06632085144519806,
-0.025659548118710518,
0.02541601099073887,
0.027241794392466545,
0.013583457097411156,
0.006697956472635269,
-0.01432524062693119,
-0.010669862851500511,
0.061375513672828674,
0.008945419453084469,
0.07906599342823029,
0.019094131886959076,
-0.0029481009114533663,
0.07872827351093292,
-0.0010895913001149893,
-0.022964172065258026,
-0.030064072459936142,
0.04343108460307121,
-0.002296643564477563,
0.04075937718153,
0.008588196709752083,
0.0050057824701070786,
-0.049221429973840714,
-0.034404970705509186,
0.05516936630010605,
-0.03692684322595596,
0.04420530050992966,
-0.0006588858086615801,
-0.0053350129164755344,
0.010521669872105122,
0.36573588848114014,
-0.07059688121080399,
-0.046225015074014664,
-0.06478925049304962,
-0.025973888114094734,
-0.004057058598846197,
-0.0018597363959997892,
-0.035697855055332184,
-0.03717609494924545,
-0.010895923711359501,
0.05061526969075203,
0.08451996743679047,
0.018933111801743507,
0.038414668291807175,
-0.07883502542972565,
-0.031168175861239433,
-0.08003866672515869,
0.06369760632514954,
-0.009690134786069393,
-0.026932161301374435,
0.06747281551361084,
-0.055447015911340714,
-0.06367471069097519,
0.03569693863391876,
-0.032089829444885254,
0.03623378276824951,
0.04351266473531723,
0.04355425387620926,
0.0727803185582161,
0.039439309388399124,
-0.00856158696115017,
-0.004164103418588638,
0.03780170530080795,
-0.03970428183674812,
0.011738787405192852,
-0.006324704736471176,
-0.027614302933216095,
0.044900089502334595,
0.01633397862315178,
0.02238832414150238,
0.04106289893388748,
-0.039761386811733246,
-0.0477081798017025,
-0.017322171479463577,
-0.04365401715040207,
0.041959427297115326,
0.09640183299779892,
0.001299743540585041,
0.008365919813513756,
-0.00489257276058197,
0.011564216576516628,
0.05270237475633621,
0.06538301706314087,
-0.039141323417425156,
0.055164214223623276,
-0.022296404466032982,
-0.04160366207361221,
0.015081197954714298,
-0.009030550718307495,
0.0015842006541788578,
-0.016120629385113716,
-0.03224527835845947,
0.02287686988711357,
0.018479444086551666,
0.055932071059942245,
0.017871994525194168,
-0.0504939965903759,
0.008348334580659866,
-0.0073072463274002075,
0.010203697718679905,
-0.010160092264413834,
0.00875882338732481,
0.043986838310956955,
-0.041717663407325745,
-0.054351698607206345,
-0.04629019275307655,
0.03181653842329979,
0.0213738065212965,
0.05980873852968216,
-0.0006125899963080883,
0.02621717005968094,
0.03543121740221977,
-0.021443720906972885,
-0.055609628558158875,
0.04089229553937912,
0.025522928684949875,
-0.09120374917984009,
0.049394115805625916,
0.02832995168864727,
0.026688940823078156,
0.05629587173461914,
-0.04477967321872711,
-0.05810466408729553,
-0.10944841057062149,
-0.030921436846256256,
0.07133155316114426,
-0.03518938645720482,
0.007197618950158358,
0.0029867265839129686,
-0.005333002656698227,
0.01145237684249878,
0.06276583671569824,
0.061807699501514435,
0.017177846282720566,
0.07573504745960236,
0.01266175601631403,
0.0841817706823349,
-0.026436420157551765,
-0.002304047578945756,
0.01921951211988926,
-0.017990028485655785,
-0.0030238740146160126,
-0.017441995441913605,
-0.056083861738443375,
-0.004577538464218378,
-0.05905343219637871,
0.038148198276758194,
0.025423113256692886,
-0.01366894319653511,
0.0468972809612751,
-0.010031729005277157,
0.034889720380306244,
-0.025347398594021797,
-0.11152997612953186,
-0.2651951313018799,
-0.055047422647476196,
0.020202038809657097,
-0.04254401475191116,
0.05491108074784279,
-0.07283096760511398,
0.015776902437210083,
-0.03623857721686363,
0.025301266461610794,
-0.042850982397794724,
0.08348746597766876,
-0.0101332301273942,
-0.003185028675943613,
-0.005017870105803013,
-0.014150337316095829,
0.03182728961110115,
0.05573133006691933,
-0.044957876205444336,
0.08172856271266937,
0.015976661816239357,
0.007512389682233334,
-0.06904060393571854,
0.06369686871767044,
0.04403586685657501,
0.05952821671962738,
-0.051408473402261734,
0.12091328203678131,
0.01124926470220089,
-0.0012975882273167372,
-0.02222198061645031,
0.030391188338398933,
0.07725631445646286,
0.008914913982152939,
-0.07339083403348923,
-0.025770626962184906,
-0.04370446130633354,
-0.026874298229813576,
-0.05182098597288132,
0.0535929836332798,
-0.008839530870318413,
-0.0165219996124506,
-0.005793417803943157,
0.013090020045638084,
-0.05704447999596596,
-0.004175826907157898,
0.009060981683433056,
0.041450854390859604,
0.027096251025795937,
-0.025343628600239754,
-0.02241375856101513,
0.05505602806806564,
-0.06045407056808472,
0.018084971234202385,
-0.00767466751858592,
-0.043974023312330246,
0.019073888659477234,
-0.02231231890618801,
-0.02432105876505375,
-0.0704946219921112,
0.03690585866570473,
-0.0496097169816494,
0.011133276857435703,
-0.015451580286026001,
0.07220456749200821,
0.008115570992231369,
-0.0009554337011650205,
0.007395763881504536,
-0.034092310816049576,
0.021319422870874405,
-0.010106000117957592,
-0.048540711402893066,
0.08285482972860336,
-0.05929926037788391,
-0.04227372258901596,
0.014838951639831066,
0.026206787675619125,
0.04741650074720383,
-0.06977123767137527,
-0.08592379093170166,
-0.014148582704365253,
0.017032429575920105,
-0.0745900347828865,
0.07451203465461731,
-0.03449564054608345,
0.007924525067210197,
0.05441873520612717,
0.01233813539147377,
-0.01654757745563984,
-0.008714837953448296,
0.02384350821375847,
0.0019329602364450693,
0.019408412277698517,
-0.05288909375667572,
-0.027446545660495758,
0.0334438793361187,
0.00522689800709486,
-0.26309478282928467,
0.042321570217609406,
0.057683128863573074,
0.018223855644464493,
0.004290909506380558,
-0.014104507863521576,
0.05406149849295616,
-0.030033281072974205,
0.02753922902047634,
0.016235101968050003,
0.02699945867061615,
0.015928812325000763,
0.0847027599811554,
0.006399256642907858,
-0.0038929961156100035,
-0.00973448809236288,
0.10704506933689117,
-0.021305223926901817,
0.05362705513834953,
0.02080574631690979,
-0.006140647456049919,
-0.01126144453883171,
0.1372208595275879,
0.03076794557273388,
0.020060330629348755,
0.07715342938899994,
-0.009929211810231209,
-0.028401801362633705,
-0.02124049887061119,
0.023702260106801987,
0.02838057652115822,
0.004394499119371176,
0.07894697785377502,
-0.05482637509703636,
-0.04361715167760849,
0.04488740488886833,
-0.008229938335716724,
0.017700057476758957,
0.03196670860052109,
0.04435471072793007,
0.03641308844089508,
-0.05031091719865799,
-0.0750323235988617,
-0.03596089407801628,
0.003293832531198859,
-0.09695897996425629,
-0.03892352804541588,
-0.0327906459569931,
-0.031088555231690407,
-0.03242957964539528,
-0.0314275324344635,
-0.0933988019824028,
0.03495035693049431,
-0.003794715041294694,
-0.038786422461271286,
0.042785629630088806,
-0.05852219834923744,
0.01824529469013214,
-0.03578722104430199,
-0.023974457755684853,
0.027309825643897057,
-0.03514380753040314,
-0.03131364285945892,
0.03155771270394325,
0.01294314581900835
] |
PMID:25080 | Metabolic changes associated with intake by cows of complete diets containing straw and concentrates in different proportions. | 1. Pelleted diets containing concentrates and 0, 200, 400 or 600 g chopped straw/kg were fed ad lib, for 5 h daily to four cows. Concentrations of various energy-yielding metabolites were measured in samples of rumen fluid and jugular blood taken before feeding and at intervals after food was offered. 2. After feeding, the pH of rumen fluid decreased rapidly and the total concentration of volatile fatty acids (VFA) increased; the changes were greatest in the diet containing no roughage. The changes were essentially complete by 135 min at approximately the time feeding stopped. Rumen lactate concentrations were always low and increased significantly only in cows given no roughage. 3. There were highly significant relationships between the peak rumen acetate concentration after a meal and the apparent digestibility of the dry matter of that meal, and the amount of material in the rumen at the end of a meal. The latter relationship resulted in there being no difference between treatments in the total amount of acetate present in the rumen after feeding. 4. In the blood, concentrations of acetate, propionate, beta-hydroxybutyrate (BHB) and lactate all increased after feeding. The increase was prolonged and maximum values were rarely reached before 4-5 h. The highest concentrations of acetate and BHB were found in cows given 20 g roughage/kg and were twice as great as those in cows given no roughage; lower concentrations were found in cows given 400 or 600 g roughage/kg. This information, together with the rumen concentrations of acetate and butyrate, was interpreted as indicating an inhibition of VFA absorption from the rumen of cows given no roughage. 5. Plasma glucose concentrations decreased rapidly for 4 h after feeding, the decrease being greatest in cows given 200 g roughage/kg. Non-esterified fatty acid concentrations also decreased after feeding 200 and 0 g roughage/kg rations, but concentrations were not high at any time. 6. It is concluded that chemical changes in the rumen correspond to feeding behaviour much more closely than changes in blood and therefore any chemostatic regulation of food intake probably occurs at the former site, with acetate playing a major role. Monitoring energy balance at tissue level is likely to be mediated hormonally, with insulin an important factor. | Metabolic changes associated with intake by cows of complete diets containing straw and concentrates in different proportions. 1. Pelleted diets containing concentrates and 0, 200, 400 or 600 g chopped straw/kg were fed ad lib, for 5 h daily to four cows. Concentrations of various energy-yielding metabolites were measured in samples of rumen fluid and jugular blood taken before feeding and at intervals after food was offered. 2. After feeding, the pH of rumen fluid decreased rapidly and the total concentration of volatile fatty acids (VFA) increased; the changes were greatest in the diet containing no roughage. The changes were essentially complete by 135 min at approximately the time feeding stopped. Rumen lactate concentrations were always low and increased significantly only in cows given no roughage. 3. There were highly significant relationships between the peak rumen acetate concentration after a meal and the apparent digestibility of the dry matter of that meal, and the amount of material in the rumen at the end of a meal. The latter relationship resulted in there being no difference between treatments in the total amount of acetate present in the rumen after feeding. 4. In the blood, concentrations of acetate, propionate, beta-hydroxybutyrate (BHB) and lactate all increased after feeding. The increase was prolonged and maximum values were rarely reached before 4-5 h. The highest concentrations of acetate and BHB were found in cows given 20 g roughage/kg and were twice as great as those in cows given no roughage; lower concentrations were found in cows given 400 or 600 g roughage/kg. This information, together with the rumen concentrations of acetate and butyrate, was interpreted as indicating an inhibition of VFA absorption from the rumen of cows given no roughage. 5. Plasma glucose concentrations decreased rapidly for 4 h after feeding, the decrease being greatest in cows given 200 g roughage/kg. Non-esterified fatty acid concentrations also decreased after feeding 200 and 0 g roughage/kg rations, but concentrations were not high at any time. 6. It is concluded that chemical changes in the rumen correspond to feeding behaviour much more closely than changes in blood and therefore any chemostatic regulation of food intake probably occurs at the former site, with acetate playing a major role. Monitoring energy balance at tissue level is likely to be mediated hormonally, with insulin an important factor. | [
0.020340897142887115,
-0.011403677985072136,
-0.01887878030538559,
0.06580906361341476,
0.07833216339349747,
0.0020202118903398514,
0.005559257697314024,
0.02043602056801319,
0.00439444137737155,
-0.02069014124572277,
0.023003168404102325,
-0.1345372200012207,
0.05269057676196098,
0.00840843841433525,
0.002656187629327178,
-0.033741895109415054,
-0.020236659795045853,
-0.0026877461932599545,
-0.09178641438484192,
-0.03509031608700752,
0.03458940610289574,
-0.027285415679216385,
-0.025109359994530678,
0.00955429021269083,
0.045922648161649704,
0.0031808766070753336,
0.013126782141625881,
0.005850580520927906,
-0.08394238352775574,
-0.20087692141532898,
0.050280410796403885,
0.01602957583963871,
0.023211129009723663,
-0.027628114446997643,
-0.055497631430625916,
0.049405403435230255,
0.023823577910661697,
-0.023853270336985588,
0.004920432344079018,
0.057490356266498566,
0.04036560654640198,
0.03645962476730347,
-0.04485038295388222,
-0.008790379390120506,
-0.06569468230009079,
0.03369414061307907,
-0.00797349214553833,
-0.009665310382843018,
0.07820861786603928,
-0.005400022491812706,
-0.053061120212078094,
-0.018794842064380646,
0.010354847647249699,
0.0334857702255249,
0.03605812415480614,
0.0005277472082525492,
0.0501512847840786,
0.054324619472026825,
0.031070685014128685,
-0.00015436176909133792,
-0.006720947567373514,
0.016978824511170387,
-0.17027312517166138,
0.05464198812842369,
0.05614401400089264,
-0.002688139211386442,
-0.06296262890100479,
-0.007917840965092182,
0.05056685954332352,
0.08867838233709335,
-0.07997694611549377,
0.03765902668237686,
-0.024952035397291183,
-0.01423905324190855,
0.020926976576447487,
-0.017170120030641556,
0.03485977277159691,
0.01904279552400112,
-0.0010264351731166244,
0.055523838847875595,
-0.028657885268330574,
0.03128515183925629,
0.009015003219246864,
-0.01720634661614895,
-0.020477090030908585,
-0.019067635759711266,
0.012972292490303516,
-0.026368089020252228,
0.01449987106025219,
0.03932807967066765,
-0.02244091033935547,
-0.06492415070533752,
0.011958293616771698,
0.028339961543679237,
0.004877534694969654,
-0.018807368353009224,
0.013972264714539051,
-0.05425659194588661,
0.021052775904536247,
0.3667583167552948,
-0.022194374352693558,
-0.0033798108343034983,
-0.018544141203165054,
-0.03781143203377724,
-0.011663674376904964,
-0.004027676768600941,
-0.023817909881472588,
0.06409555673599243,
0.02492232248187065,
0.0639658197760582,
0.03994947299361229,
0.019022930413484573,
0.0002047833550022915,
0.008027424104511738,
-0.013449497520923615,
-0.002025758847594261,
0.03054264560341835,
0.0290924534201622,
-0.021255889907479286,
0.01859821192920208,
-0.007536553777754307,
0.007360411342233419,
0.045502670109272,
0.0008538338588550687,
0.04738686606287956,
0.04756758734583855,
-0.018045485019683838,
0.088929682970047,
0.027372710406780243,
0.005172801669687033,
-0.010788905434310436,
-0.0560854896903038,
-0.06392637640237808,
-0.029975485056638718,
-0.002073699375614524,
-0.021470483392477036,
0.06159511208534241,
-0.008201614022254944,
0.029861582443118095,
0.07464811205863953,
-0.04736282676458359,
-0.07475737482309341,
-0.013918809592723846,
-0.1456441879272461,
-0.025531744584441185,
0.12024882435798645,
0.10059840977191925,
-0.004561441019177437,
-0.05879763141274452,
-0.019820652902126312,
0.0377143993973732,
0.05129348859190941,
0.006523114163428545,
0.004580006003379822,
0.03284991905093193,
0.024950673803687096,
-0.02354227751493454,
-0.025003887712955475,
0.014006474055349827,
-0.04588962346315384,
-0.04582728073000908,
-0.04054601863026619,
-0.05480218306183815,
-0.00221137679181993,
0.03010275401175022,
-0.015581087209284306,
-0.06682562828063965,
0.023230621591210365,
-0.0024247204419225454,
0.016910115256905556,
0.04213510826230049,
-0.010322523303329945,
-0.05380301922559738,
-0.04624267295002937,
0.01589137874543667,
-0.02967432513833046,
0.03317740932106972,
0.028536707162857056,
-0.01728919893503189,
-0.011373578570783138,
0.09182430803775787,
-0.013037133030593395,
-0.01187649555504322,
0.04149944335222244,
0.012017941102385521,
-0.025956127792596817,
-0.07380963861942291,
0.010898840613663197,
0.03395037725567818,
0.029128680005669594,
-0.0317688025534153,
-0.019079698249697685,
-0.06371679902076721,
-0.006090033333748579,
-0.04633639007806778,
-0.05536801367998123,
-0.009102515876293182,
0.04823232442140579,
-0.021376796066761017,
0.0021762719843536615,
0.0456281341612339,
0.0005880323005840182,
0.01724187843501568,
0.055402059108018875,
0.05015590041875839,
0.055907610803842545,
-0.04519584774971008,
0.022946441546082497,
0.07267609238624573,
-0.005672531668096781,
0.017090613022446632,
0.018578791990876198,
-0.054877690970897675,
0.03898124024271965,
-0.11565931886434555,
0.0791327953338623,
0.024773957207798958,
-0.026103876531124115,
0.03338233381509781,
-0.018807347863912582,
0.08947983384132385,
-0.09551189839839935,
-0.006022003013640642,
-0.3245718479156494,
-0.02811143361032009,
0.0191228985786438,
0.00007402223127428442,
0.04811416193842888,
0.021010154858231544,
-0.025447502732276917,
-0.005378521513193846,
0.007187167648226023,
-0.00967355165630579,
-0.007304825354367495,
0.026248712092638016,
-0.05374421551823616,
-0.00531799579039216,
0.09270182996988297,
0.02420821413397789,
0.03918519243597984,
-0.06476052850484848,
-0.018043896183371544,
-0.0045255934819579124,
0.006364328321069479,
0.012560083530843258,
0.016580812633037567,
0.017216958105564117,
0.06557605415582657,
0.017214706167578697,
0.11065692454576492,
-0.0479067899286747,
0.01069631613790989,
-0.0018389275064691901,
-0.031210754066705704,
0.009936097078025341,
-0.01039199996739626,
-0.053880151361227036,
-0.020336095243692398,
-0.02657124027609825,
-0.012052907608449459,
-0.02326616272330284,
0.020457664504647255,
-0.03175084665417671,
0.0029069632291793823,
0.011059856042265892,
-0.017741722986102104,
-0.07959011197090149,
-0.012138091959059238,
-0.0071915932931005955,
-0.00797926913946867,
-0.034066103398799896,
-0.01584743894636631,
-0.02173811011016369,
0.03644349053502083,
-0.0015703238314017653,
0.00008895542850950733,
-0.0038029635325074196,
-0.01414632797241211,
0.0014041472459211946,
-0.021454185247421265,
-0.015241105109453201,
-0.05788945406675339,
0.010407173074781895,
-0.04380268603563309,
-0.011604192666709423,
-0.0025763260200619698,
-0.013365434482693672,
-0.032895877957344055,
0.017530713230371475,
-0.04043802618980408,
-0.024135958403348923,
0.017484955489635468,
0.056394148617982864,
0.030320782214403152,
0.06635534018278122,
-0.03259047493338585,
-0.042934466153383255,
0.057775743305683136,
0.0365687794983387,
-0.06671122461557388,
-0.028246983885765076,
-0.07983949780464172,
0.02388088032603264,
0.06677614152431488,
-0.0749322921037674,
0.05518198385834694,
0.004055413883179426,
0.026926470920443535,
0.01554073765873909,
0.03549182787537575,
-0.07933849096298218,
0.05048691853880882,
0.028421370312571526,
-0.026975443586707115,
-0.02645849622786045,
-0.029882105067372322,
-0.05916445329785347,
0.06763030588626862,
0.06593713164329529,
-0.26120689511299133,
0.028570635244250298,
-0.0065428996458649635,
-0.012646082788705826,
0.03275703638792038,
0.03140142187476158,
0.014558693394064903,
0.004760194104164839,
0.003709645476192236,
-0.018138153478503227,
0.005066064186394215,
0.04954215884208679,
0.04688160493969917,
0.03707638755440712,
-0.00442975340411067,
0.03292868286371231,
0.011499164626002312,
0.04927724972367287,
-0.018765820190310478,
-0.0367104634642601,
0.003774402430281043,
-0.082270048558712,
0.12927493453025818,
-0.0015345655847340822,
-0.03895489126443863,
-0.002825554460287094,
-0.003439555177465081,
0.009164138697087765,
0.021669955924153328,
0.008571239188313484,
0.00419551832601428,
0.019548436626791954,
0.13869674503803253,
-0.020807510241866112,
-0.0025166221894323826,
0.06065927818417549,
0.0023745261132717133,
-0.0011109168408438563,
-0.01831429824233055,
0.009453961625695229,
-0.00535963661968708,
-0.038869645446538925,
-0.045175544917583466,
-0.033590953797101974,
0.010602372698485851,
-0.05959480628371239,
0.0037316549569368362,
-0.09255471080541611,
-0.0037694498896598816,
0.028368212282657623,
0.018229570239782333,
-0.03002159669995308,
0.006912576034665108,
0.015269230119884014,
0.035447899252176285,
0.014767962507903576,
-0.013214274309575558,
-0.004915689118206501,
-0.0626121535897255,
-0.014777025207877159,
0.04119483381509781,
0.008897241204977036,
-0.023420998826622963,
0.03333595395088196,
0.021484244614839554
] |
PMID:25081 | On the measurement of pH in Escherichia coli by 31P nuclear magnetic resonance. | The 31P high resolution NMR spectra of concentrated suspensions of Escherichia coli cells have been measured at 145.8 MHz. The position of the orthophosphate resonance is used as a measure of internal and external pH. In accord with Paddan, Zilberstein and Rottenberg ((1976) Eur. J. Biochem. 63, 533--541) it is shown that when properly energized the internal pH is 7.5 +/- 0.1. By synchronizing the NMR data acquisition with 3-s bursts of O2 it is possible to measure the internal pH with a time resolution of about 1 s. It is shown that at 20 degrees C the pH remains constant for times longer than 15 s after the oxygen is discontinued and it decays in several minutes. | On the measurement of pH in Escherichia coli by 31P nuclear magnetic resonance. The 31P high resolution NMR spectra of concentrated suspensions of Escherichia coli cells have been measured at 145.8 MHz. The position of the orthophosphate resonance is used as a measure of internal and external pH. In accord with Paddan, Zilberstein and Rottenberg ((1976) Eur. J. Biochem. 63, 533--541) it is shown that when properly energized the internal pH is 7.5 +/- 0.1. By synchronizing the NMR data acquisition with 3-s bursts of O2 it is possible to measure the internal pH with a time resolution of about 1 s. It is shown that at 20 degrees C the pH remains constant for times longer than 15 s after the oxygen is discontinued and it decays in several minutes. | [
-0.10053923726081848,
0.028003258630633354,
-0.01001068390905857,
-0.03890082240104675,
0.03814360499382019,
-0.04908061772584915,
0.036345817148685455,
0.03507265821099281,
0.00783760566264391,
-0.015300603583455086,
0.008990312926471233,
-0.11012622714042664,
0.019088702276349068,
-0.007705043535679579,
-0.02186843566596508,
-0.0384708046913147,
-0.05375911667943001,
-0.013807810842990875,
-0.0036294525489211082,
0.013427933678030968,
0.018490107730031013,
-0.05752846226096153,
-0.02155984193086624,
0.012616114690899849,
0.050460297614336014,
0.03268682211637497,
0.003803201951086521,
-0.010543858632445335,
-0.09142027795314789,
-0.20515497028827667,
-0.00307660223916173,
-0.06243258714675903,
0.03589679300785065,
-0.05997740849852562,
-0.03267384693026543,
0.01915774494409561,
-0.010706895962357521,
-0.03071741573512554,
-0.004264994524419308,
0.024479275569319725,
0.04778486490249634,
0.026000862941145897,
0.028405848890542984,
0.02009802870452404,
-0.02874949388206005,
-0.06703247874975204,
-0.037014592438936234,
-0.021733347326517105,
0.08741380274295807,
0.057338230311870575,
-0.0427689403295517,
0.050163961946964264,
-0.020687928423285484,
0.03235567733645439,
-0.023046553134918213,
-0.015799177810549736,
-0.003899530041962862,
-0.007700755726546049,
0.0009146237280219793,
-0.01245882362127304,
0.06052665784955025,
-0.012532195076346397,
-0.15918025374412537,
0.053526148200035095,
0.05600705370306969,
-0.029942743480205536,
-0.022323230281472206,
0.018750352784991264,
0.09506521373987198,
0.05128943547606468,
-0.048127759248018265,
0.012522052973508835,
-0.036118656396865845,
0.0025105879176408052,
0.013292640447616577,
-0.03042207658290863,
0.030546091496944427,
-0.015942739322781563,
0.008872635662555695,
-0.0011374049354344606,
-0.017513815313577652,
-0.0007095490000210702,
0.005819896701723337,
-0.05474068969488144,
-0.010220889002084732,
0.028993863612413406,
0.03372868895530701,
-0.037295013666152954,
0.02167704701423645,
0.05162034556269646,
-0.017405912280082703,
-0.06607925891876221,
-0.012022522278130054,
0.023199114948511124,
-0.012071575038135052,
0.04351077228784561,
-0.009269826114177704,
-0.02764461189508438,
0.02160107158124447,
0.4012219309806824,
-0.025509856641292572,
0.016794724389910698,
0.0217109564691782,
-0.020816979929804802,
0.013142222538590431,
-0.016129275783896446,
0.026654528453946114,
0.048915233463048935,
-0.06375820189714432,
0.04784146323800087,
-0.03452308103442192,
0.01353050023317337,
0.050358451902866364,
-0.06309300661087036,
-0.04733752831816673,
-0.03404601663351059,
0.06414835155010223,
-0.019108645617961884,
-0.04092853516340256,
0.01789817586541176,
0.010752342641353607,
0.012650909833610058,
-0.010376780293881893,
-0.03873147442936897,
0.06833269447088242,
-0.05210161209106445,
-0.00627032108604908,
0.07886117696762085,
0.0016792756505310535,
0.03500330075621605,
-0.009714288637042046,
0.0010591793106868863,
-0.01451891753822565,
0.012048320844769478,
0.017328394576907158,
0.0015420145355165005,
-0.003366600489243865,
-0.017742158845067024,
0.09799234569072723,
-0.001561610260978341,
-0.01725572533905506,
-0.05740201845765114,
-0.061613451689481735,
-0.06047457456588745,
-0.08751626312732697,
0.07522380352020264,
-0.04433320835232735,
0.04525844007730484,
-0.06821523606777191,
-0.022815588861703873,
-0.016671065241098404,
0.11616268754005432,
-0.021440403535962105,
-0.0026578789111226797,
0.019827507436275482,
0.019083475694060326,
0.0025372235104441643,
-0.021677320823073387,
0.006748688407242298,
-0.06137365475296974,
-0.08666589856147766,
-0.01187566202133894,
0.016816414892673492,
0.07224398851394653,
0.0012269519502297044,
-0.012227281928062439,
-0.032727476209402084,
-0.013933793641626835,
-0.0017559919506311417,
-0.017779560759663582,
0.049355704337358475,
-0.03518669679760933,
0.012769224122166634,
-0.0809590145945549,
0.0012908553471788764,
-0.005450988654047251,
0.001951176323927939,
0.08144570887088776,
0.02700578235089779,
-0.022631783038377762,
0.004112765658646822,
-0.004421742632985115,
0.023271700367331505,
0.011547144502401352,
0.07024548947811127,
-0.038044147193431854,
-0.06632108241319656,
0.008864989504218102,
-0.05754657834768295,
0.06668973714113235,
0.0005005258717574179,
-0.015932096168398857,
-0.0545336939394474,
0.04684171825647354,
-0.04914747551083565,
-0.039585646241903305,
-0.0032461329828947783,
0.07092490047216415,
-0.0005441457033157349,
0.0032471742015331984,
0.07453621178865433,
-0.022881364449858665,
-0.021033721044659615,
0.01078549213707447,
0.00519783329218626,
0.029674621298909187,
0.0636797770857811,
0.0950847864151001,
0.038620803505182266,
0.016689639538526535,
-0.043128520250320435,
-0.0022706789895892143,
-0.04963194951415062,
-0.016727225854992867,
-0.032996173948049545,
-0.0015145521610975266,
0.04380541667342186,
0.017322545871138573,
0.03282599896192551,
0.03227894753217697,
0.02483828365802765,
-0.06685217469930649,
-0.06968887895345688,
-0.2711438834667206,
0.025354381650686264,
0.03193439170718193,
0.023966310545802116,
0.0956934317946434,
-0.08490034192800522,
-0.005019200965762138,
-0.03032020479440689,
0.09278810769319534,
-0.06421320885419846,
0.008168716914951801,
0.04570263996720314,
-0.07059822231531143,
-0.028942447155714035,
0.019236017018556595,
0.03472164273262024,
0.07787307351827621,
-0.01952468231320381,
0.03498271107673645,
-0.006220764946192503,
0.012738145887851715,
0.05286254361271858,
-0.04253435879945755,
0.04644540324807167,
0.02646031603217125,
-0.02754068188369274,
0.0920383483171463,
-0.025553205981850624,
0.009754600003361702,
0.02922496758401394,
0.002570308279246092,
0.0441296249628067,
0.027096595615148544,
-0.06336874514818192,
-0.07671135663986206,
0.018412526696920395,
-0.010529536753892899,
-0.027531705796718597,
0.03296931833028793,
-0.010798569768667221,
-0.01126785110682249,
-0.027042226865887642,
-0.05295056477189064,
-0.03204566612839699,
-0.007296413648873568,
-0.010910417884588242,
0.015260498970746994,
0.039487436413764954,
-0.0068947309628129005,
-0.010555888526141644,
0.044536031782627106,
0.011099344119429588,
-0.030021045356988907,
-0.04647912085056305,
-0.01244489848613739,
0.01959611102938652,
0.0014183773892000318,
-0.023792747408151627,
-0.04057556763291359,
-0.05089200660586357,
-0.009755181148648262,
0.02228005789220333,
-0.010192335583269596,
0.027100669220089912,
0.006076992489397526,
-0.041325658559799194,
0.03775123506784439,
0.025401510298252106,
0.02913203276693821,
0.03744169697165489,
0.036178916692733765,
0.059725649654865265,
-0.011331338435411453,
0.028095629066228867,
0.03692445158958435,
0.00979722198098898,
-0.023279255256056786,
-0.06626851111650467,
-0.00539688766002655,
-0.0003684064431581646,
0.051119379699230194,
0.012291430495679379,
0.07629508525133133,
-0.051783300936222076,
-0.007155600469559431,
0.058958668261766434,
0.007495055440813303,
-0.05068368464708328,
-0.007842488586902618,
0.06287381798028946,
0.02212947979569435,
0.01029224693775177,
-0.018631109967827797,
-0.05498543009161949,
0.01743835024535656,
0.017282899469137192,
-0.2782400846481323,
0.008681689389050007,
0.03619164600968361,
0.0427822545170784,
-0.019153565168380737,
0.0785301923751831,
0.009673705324530602,
0.009951959364116192,
-0.05345451086759567,
0.10257697850465775,
0.013077344745397568,
0.012042071670293808,
0.03657716140151024,
0.03947645053267479,
0.02722649648785591,
0.07496534287929535,
-0.02713429369032383,
-0.03385248780250549,
0.0204616766422987,
-0.055768389254808426,
0.004463414661586285,
-0.020185958594083786,
0.14218854904174805,
-0.021753888577222824,
-0.015259535983204842,
0.062360670417547226,
0.02191106602549553,
0.027816584333777428,
-0.020390674471855164,
-0.024261686950922012,
0.002542781876400113,
0.028885217383503914,
0.04926742613315582,
-0.030003316700458527,
0.05003044009208679,
0.061346329748630524,
-0.01528177410364151,
0.016714559867978096,
-0.0408092699944973,
-0.001490373513661325,
0.020144464448094368,
0.013033724389970303,
-0.0625310093164444,
-0.012615691870450974,
0.045156385749578476,
-0.015693122521042824,
-0.03972573205828667,
-0.02818150632083416,
0.01056565623730421,
-0.04951830953359604,
-0.04845251142978668,
0.029520226642489433,
0.03937826678156853,
0.03034995310008526,
-0.002262852154672146,
0.05599876120686531,
-0.03857480362057686,
-0.06877300888299942,
-0.0476575531065464,
-0.033012088388204575,
0.03424976021051407,
0.03269021213054657,
0.00170418294146657,
0.0423302985727787,
-0.016044752672314644
] |
PMID:25082 | A study of H+ transport in gastric microsomal vesicles using fluorescent probes. | Fluorescent amines, 9-aminoacridine, acridine orange and quinacrine, were used as probes for a pH gradient (deltapH) across gastric microsomal vesicles. Analysis of probe uptake data indicates that 9-aminoacridine distributes across the membrane as a weak base in accordance with the deltapH. On the other hand, acridine orange and quinacrine show characteristics of binding to membrane sites in addition to the accumulation in response to deltapH. A discussion of the advantages and limitations of the probes is presented. Application of these probes to pig gastric microsomal vesicles indicates that that K+-stimulated ATPase is responsible for the transport of H+ into the vesicles and thus develops a deltapH across the membrane. The deltapH generated by the K+-ATPase has a definite requirement for internal K+. The proton gradient can be discharged slowly after ATP depletion or rapidly either by detergent disruption of the vesicles or by increasing their leakiness using both H+ and K+ ionophores. On the other hand, the sole use of the K+ ionophore, valinomycin, stimulates the ATP-induced formation of deltapH by increasing the availability of K+ to internal sites. This stimulation by valinomycin requires the presence of permeable anions like Cl-. Analysis of the Cl- requirement indicates that in the presence of valinomycin the net effect is the accumulation of HCl inside the gastric vesicles. With an external pH of 7.0, the ATP-generated deltapH was calculated to be from 4 to 4.5 pH units. The results are consistent with the hypothesis that the K+-stimulated ATPase drives a K+/H+ exchange across the gastric vesicles. Since other lines of evidence suggest that these gastric microsomes are derived from the tubulovesicular system of the oxyntic cell, the participation of the ATP-driven transport processes in gastric HCl secretion is of interest. | A study of H+ transport in gastric microsomal vesicles using fluorescent probes. Fluorescent amines, 9-aminoacridine, acridine orange and quinacrine, were used as probes for a pH gradient (deltapH) across gastric microsomal vesicles. Analysis of probe uptake data indicates that 9-aminoacridine distributes across the membrane as a weak base in accordance with the deltapH. On the other hand, acridine orange and quinacrine show characteristics of binding to membrane sites in addition to the accumulation in response to deltapH. A discussion of the advantages and limitations of the probes is presented. Application of these probes to pig gastric microsomal vesicles indicates that that K+-stimulated ATPase is responsible for the transport of H+ into the vesicles and thus develops a deltapH across the membrane. The deltapH generated by the K+-ATPase has a definite requirement for internal K+. The proton gradient can be discharged slowly after ATP depletion or rapidly either by detergent disruption of the vesicles or by increasing their leakiness using both H+ and K+ ionophores. On the other hand, the sole use of the K+ ionophore, valinomycin, stimulates the ATP-induced formation of deltapH by increasing the availability of K+ to internal sites. This stimulation by valinomycin requires the presence of permeable anions like Cl-. Analysis of the Cl- requirement indicates that in the presence of valinomycin the net effect is the accumulation of HCl inside the gastric vesicles. With an external pH of 7.0, the ATP-generated deltapH was calculated to be from 4 to 4.5 pH units. The results are consistent with the hypothesis that the K+-stimulated ATPase drives a K+/H+ exchange across the gastric vesicles. Since other lines of evidence suggest that these gastric microsomes are derived from the tubulovesicular system of the oxyntic cell, the participation of the ATP-driven transport processes in gastric HCl secretion is of interest. | [
-0.04069862142205238,
-0.037491653114557266,
-0.010589413344860077,
0.06251763552427292,
-0.005590308923274279,
-0.01061423122882843,
0.008726867847144604,
0.01576639711856842,
-0.02686525695025921,
-0.024212801828980446,
0.02940346859395504,
-0.08613517135381699,
-0.03033246099948883,
0.049797747284173965,
0.011378616094589233,
-0.07203397154808044,
-0.0019301262218505144,
-0.03479504585266113,
-0.041667867451906204,
-0.021818973124027252,
-0.028317533433437347,
-0.053661495447158813,
0.011253176257014275,
-0.01078492496162653,
0.014359419234097004,
0.008455468341708183,
0.025593223050236702,
-0.041172876954078674,
-0.08519405871629715,
-0.2026059329509735,
-0.010494601912796497,
-0.045858852565288544,
-0.03798593580722809,
-0.00709689874202013,
-0.05047222599387169,
-0.015467091463506222,
0.004879532381892204,
0.006111416965723038,
0.004477770999073982,
0.030178235843777657,
0.04216079041361809,
-0.02495792880654335,
0.023845208808779716,
0.01748998649418354,
-0.015982890501618385,
-0.0692160502076149,
-0.009975469671189785,
0.020449617877602577,
0.07596447318792343,
0.014984059147536755,
0.012394677847623825,
0.02670414187014103,
0.018531223759055138,
0.02750563621520996,
0.0575389489531517,
0.025499574840068817,
-0.0007252741488628089,
0.047063376754522324,
-0.001310159103013575,
-0.030745364725589752,
0.07893902063369751,
0.06110098585486412,
-0.16666476428508759,
0.01618286408483982,
0.09397592395544052,
-0.029118964448571205,
-0.004214412532746792,
-0.03479112312197685,
0.0774274468421936,
0.08575420826673508,
-0.02812180109322071,
-0.02184302918612957,
-0.013769666664302349,
0.03875081613659859,
0.02080676145851612,
-0.060215339064598083,
0.007461824454367161,
-0.04070279747247696,
0.014935244806110859,
-0.032777849584817886,
-0.0018817407544702291,
-0.015835151076316833,
0.02419232204556465,
-0.042728278785943985,
-0.01805289275944233,
0.017772244289517403,
-0.0016330174403265119,
-0.018037399277091026,
0.003485677530989051,
0.017927609384059906,
0.013724264688789845,
-0.08053459972143173,
-0.044936880469322205,
-0.005641432944685221,
-0.05541635677218437,
0.01823490858078003,
0.02101357840001583,
-0.03635140135884285,
-0.03488275408744812,
0.39351338148117065,
-0.01648181490600109,
0.030810406431555748,
-0.025728797540068626,
-0.00615621916949749,
-0.018156535923480988,
0.019415367394685745,
0.008858362212777138,
-0.009926702827215195,
0.00795426033437252,
0.010250944644212723,
0.03567797318100929,
0.016550205647945404,
0.05339355021715164,
-0.04126223921775818,
-0.06677866727113724,
0.045870523899793625,
0.0305161289870739,
0.00298062851652503,
0.007065544370561838,
0.04417266696691513,
-0.047965891659259796,
-0.013081392273306847,
0.02774002030491829,
-0.00892798975110054,
0.06606797128915787,
-0.027276266366243362,
-0.07410545647144318,
0.06370292603969574,
0.02260729856789112,
0.028035152703523636,
0.049599386751651764,
0.051965564489364624,
-0.030544279143214226,
-0.015370208770036697,
-0.009216024540364742,
-0.05591326951980591,
0.006961963139474392,
-0.03664868697524071,
0.03525600954890251,
-0.019973618909716606,
-0.08698629587888718,
-0.0026373614091426134,
-0.03567039221525192,
-0.10622148960828781,
-0.08346237987279892,
0.10317207872867584,
0.0026911140885204077,
0.02229500003159046,
-0.01021425798535347,
0.0071107796393334866,
0.05078749358654022,
0.023367036134004593,
0.004507750738412142,
0.06297256052494049,
-0.027068009600043297,
0.03365439549088478,
-0.01982150226831436,
0.0346749983727932,
-0.02158193476498127,
0.06292858719825745,
-0.06587612628936768,
-0.07424743473529816,
-0.03704918920993805,
0.09657835960388184,
0.024918202310800552,
-0.031009789556264877,
0.031213464215397835,
0.00241862959228456,
-0.021938996389508247,
-0.032088857144117355,
0.04128646105527878,
-0.010089569725096226,
-0.006400992628186941,
0.006204187870025635,
-0.04200506955385208,
-0.00914886686950922,
-0.02848324179649353,
0.02662474848330021,
0.0008930823532864451,
-0.05449340492486954,
0.0036993608810007572,
-0.015073766000568867,
-0.07310260087251663,
0.008523491211235523,
0.044033970683813095,
-0.013167439959943295,
-0.04107941687107086,
0.02537648379802704,
-0.019328981637954712,
0.1027926653623581,
-0.04159529134631157,
0.03218916431069374,
-0.04183448106050491,
0.007528113201260567,
-0.03063531033694744,
-0.059614166617393494,
-0.01275940053164959,
0.050020694732666016,
-0.03661995753645897,
-0.0031235862988978624,
0.06707324087619781,
0.017738422378897667,
0.011467552743852139,
0.06362728774547577,
-0.012310601770877838,
0.007435899693518877,
0.0021316988859325647,
0.013124259188771248,
0.055336952209472656,
-0.014624178409576416,
0.01464079786092043,
-0.00818690937012434,
-0.051428355276584625,
0.018824448809027672,
-0.055366892367601395,
0.010348381474614143,
-0.01598154753446579,
0.06850411742925644,
0.06579297035932541,
0.017328409478068352,
0.0631173625588417,
-0.0549030639231205,
-0.05039706081151962,
-0.2880280315876007,
-0.04637055844068527,
0.035442568361759186,
0.014361144043505192,
0.10645990818738937,
-0.02806589938700199,
0.024559687823057175,
0.004041830077767372,
0.03381898254156113,
-0.06753949820995331,
0.03405415639281273,
0.03369952365756035,
-0.056636642664670944,
-0.016245746985077858,
0.02623281069099903,
0.04016859084367752,
0.0218646340072155,
-0.0171615332365036,
0.026973720639944077,
-0.009307287633419037,
0.03195809945464134,
-0.035029925405979156,
-0.007554327137768269,
0.004480711184442043,
0.02239253558218479,
-0.03430214151740074,
0.11482319980859756,
0.04914373904466629,
-0.006552813109010458,
-0.06989683210849762,
-0.009001760743558407,
0.056391190737485886,
-0.00043708464363589883,
-0.09021996706724167,
-0.03265797719359398,
-0.044168103486299515,
-0.005346899852156639,
-0.006875421851873398,
0.039313845336437225,
-0.023617209866642952,
-0.057192642241716385,
0.01202654093503952,
-0.03757096081972122,
-0.0607166588306427,
0.02142595872282982,
-0.028873099014163017,
-0.00010437370656291023,
0.0289201270788908,
0.004552362021058798,
-0.04213501885533333,
0.07338723540306091,
0.008750873617827892,
-0.0076467194594442844,
-0.01811271905899048,
0.028180310502648354,
-0.03374894708395004,
0.048803120851516724,
-0.004808553494513035,
-0.08219528198242188,
-0.017293162643909454,
0.013882794417440891,
0.03034113347530365,
-0.0018038011621683836,
0.016638249158859253,
0.01479727029800415,
-0.008544621989130974,
0.023144608363509178,
0.04012782871723175,
0.05021139979362488,
-0.013442927971482277,
0.06136366352438927,
0.1321939080953598,
-0.034386198967695236,
0.07477100193500519,
0.07483209669589996,
0.049399085342884064,
0.019969189539551735,
-0.006246392149478197,
-0.07608994841575623,
0.003903019241988659,
0.01578802615404129,
-0.04968322068452835,
0.04183857887983322,
-0.022670626640319824,
-0.0750034898519516,
0.07422269880771637,
0.018496457487344742,
-0.02082737535238266,
0.04012175649404526,
0.015754347667098045,
-0.02026207372546196,
0.015316479839384556,
-0.04001001641154289,
-0.05065668746829033,
0.001726623740978539,
0.02178080938756466,
-0.26083794236183167,
0.029776105657219887,
0.011242076754570007,
0.014517043717205524,
-0.04346426576375961,
0.026690317317843437,
0.05207587778568268,
-0.04490279406309128,
-0.01669924147427082,
0.015420093201100826,
0.01708095520734787,
-0.018965674564242363,
0.08465371280908585,
0.06780590862035751,
-0.024584831669926643,
-0.0036939806304872036,
0.07209452986717224,
-0.04110252484679222,
-0.0172627791762352,
0.017230674624443054,
0.0477091483771801,
-0.020947366952896118,
0.1302400529384613,
-0.038925886154174805,
0.022098705172538757,
0.04564260318875313,
0.0388227179646492,
0.03181227669119835,
0.027539454400539398,
-0.010063553228974342,
-0.001779421349056065,
0.0533067025244236,
0.03766961395740509,
-0.04100104421377182,
0.05469105392694473,
-0.00010158008808502927,
-0.02522432990372181,
-0.0004720333672594279,
-0.08024284243583679,
0.003288548206910491,
0.048175591975450516,
0.023722708225250244,
-0.036296989768743515,
-0.010780568234622478,
0.0519905649125576,
-0.07054591923952103,
-0.027771033346652985,
-0.031494926661252975,
0.03293672949075699,
-0.04730856046080589,
0.010324710048735142,
-0.033789217472076416,
0.02647922746837139,
0.0628180280327797,
0.010646008886396885,
0.0035561465192586184,
-0.039320703595876694,
-0.012522580102086067,
0.0035135792568325996,
0.008828576654195786,
0.02231951430439949,
-0.03272615373134613,
-0.00788893923163414,
0.05488205701112747,
0.02286534383893013
] |
PMID:25083 | NMR kinetic studies of the ionophore X-537A-mediated transport of manganous ions across phospholipid bilayers. | Nuclear magnetic resonance spectroscopy has been applied as a method for studying manganous ions transport across the membrane of phosphatidylcholine vesicles. The rates of the ionophore X-537A (lasalocid A)-mediated Mn2+ transport have been measured as a function of ionophore concentration, pH of the vesicle suspension, and temperature. The translocation was found to occur via a neutral complex composed of one manganous ion bound in two ionized X-537A molecules (Mn X2). The activation energy for the overall transport process was determined to be 22 +/- 5 kcal/mol. Also a pKa of 5.0 +/- 0.2 was determined for the ionophore acid dissociation equilibrium in the vesicle suspension. | NMR kinetic studies of the ionophore X-537A-mediated transport of manganous ions across phospholipid bilayers. Nuclear magnetic resonance spectroscopy has been applied as a method for studying manganous ions transport across the membrane of phosphatidylcholine vesicles. The rates of the ionophore X-537A (lasalocid A)-mediated Mn2+ transport have been measured as a function of ionophore concentration, pH of the vesicle suspension, and temperature. The translocation was found to occur via a neutral complex composed of one manganous ion bound in two ionized X-537A molecules (Mn X2). The activation energy for the overall transport process was determined to be 22 +/- 5 kcal/mol. Also a pKa of 5.0 +/- 0.2 was determined for the ionophore acid dissociation equilibrium in the vesicle suspension. | [
-0.09390542656183243,
-0.02323424071073532,
-0.007235846947878599,
-0.021619299426674843,
0.0029327417723834515,
-0.015542936511337757,
0.06163213029503822,
0.011431330814957619,
-0.05562910810112953,
-0.03914729878306389,
0.03822477534413338,
-0.0861467644572258,
0.005641174037009478,
0.039396870881319046,
0.048933934420347214,
-0.0026425218675285578,
-0.044387221336364746,
-0.041698429733514786,
-0.032307155430316925,
0.004996682051569223,
0.0204952210187912,
-0.04905225709080696,
0.009502673521637917,
-0.02799287624657154,
0.06370289623737335,
0.052186641842126846,
0.001434221863746643,
0.0008998488774523139,
-0.0927177220582962,
-0.20018969476222992,
-0.02087564207613468,
-0.02397192269563675,
-0.01673567295074463,
-0.031246347352862358,
-0.04962823539972305,
0.00941507238894701,
-0.009497025050222874,
0.0022373150568455458,
-0.0011915236245840788,
0.022364838048815727,
0.04667113348841667,
-0.0012835233937948942,
0.01855226792395115,
0.02550848200917244,
-0.013630285859107971,
-0.031080812215805054,
-0.04953242465853691,
0.024516085162758827,
0.12306323647499084,
0.036282967776060104,
0.013559825718402863,
0.03817684203386307,
0.04134170338511467,
0.028301194310188293,
0.004576514475047588,
0.020124392583966255,
-0.003152773715555668,
-0.004619958344846964,
-0.0340084545314312,
0.016161374747753143,
0.031090036034584045,
0.0007370714447461069,
-0.14660552144050598,
0.12811140716075897,
0.029780210927128792,
-0.02364683523774147,
-0.03675928711891174,
-0.011476969346404076,
0.050008464604616165,
0.016934841871261597,
-0.056645914912223816,
0.03310664743185043,
-0.06061814725399017,
0.012298235669732094,
0.06506926566362381,
-0.017638904973864555,
0.001256100251339376,
0.022208653390407562,
0.03225943446159363,
-0.008555377833545208,
-0.04499027878046036,
0.01911814883351326,
-0.010295090265572071,
-0.028019247576594353,
-0.02420358546078205,
0.04615114629268646,
0.026113182306289673,
-0.0073107946664094925,
0.0040502529591321945,
0.010016154497861862,
-0.03266811743378639,
-0.03231451287865639,
-0.047527212649583817,
0.012324024923145771,
-0.07245088368654251,
0.08316943049430847,
-0.01453008595854044,
-0.00668199872598052,
0.007014484144747257,
0.3664554953575134,
-0.0016081648645922542,
0.048254698514938354,
0.003013101639226079,
0.019843077287077904,
-0.008658583275973797,
-0.020281363278627396,
0.001066153054125607,
-0.024900533258914948,
-0.0030964347533881664,
0.029384486377239227,
0.057999104261398315,
0.003837969619780779,
0.07943159341812134,
-0.07986866682767868,
-0.05042727291584015,
0.0029267810750752687,
0.058498356491327286,
-0.002291545970365405,
-0.0240024384111166,
0.06913778930902481,
-0.06910113245248795,
-0.0065972995944321156,
0.022064508870244026,
0.023376181721687317,
0.04463038593530655,
-0.0037350847851485014,
-0.038906730711460114,
0.0577424056828022,
0.03111506998538971,
-0.0009987188968807459,
0.035026490688323975,
0.06580793112516403,
-0.029524102807044983,
-0.034198928624391556,
-0.0004233454819768667,
-0.026662515476346016,
0.03942627087235451,
-0.04885702580213547,
0.001632366213016212,
-0.040623780339956284,
-0.02950681373476982,
-0.006314933765679598,
-0.03394528105854988,
-0.0760989785194397,
-0.0448598638176918,
0.07658395916223526,
0.02438213862478733,
0.04762369021773338,
-0.003617658047005534,
0.02506149560213089,
0.03415056690573692,
0.03168502822518349,
-0.034069061279296875,
-0.027847662568092346,
-0.04128480330109596,
-0.013347403146326542,
-0.01619792729616165,
-0.011926427483558655,
-0.009228617884218693,
-0.009025746956467628,
-0.0341956801712513,
-0.05671663209795952,
-0.03650018200278282,
0.11375411599874496,
0.008044980466365814,
-0.05793764069676399,
0.010679108090698719,
-0.00918428972363472,
-0.014252673834562302,
-0.0042781648226082325,
-0.014756409451365471,
0.02424350380897522,
-0.04463403299450874,
-0.06682033836841583,
-0.022809403017163277,
-0.003913941327482462,
0.009771876037120819,
-0.015443691052496433,
-0.017080096527934074,
-0.05571112036705017,
0.02968771569430828,
-0.0373731330037117,
-0.015506400726735592,
0.008850487880408764,
0.05468768626451492,
0.023506982252001762,
-0.07281683385372162,
0.030355026945471764,
-0.03343429043889046,
0.03146565333008766,
-0.046747591346502304,
-0.01092641893774271,
0.026628268882632256,
0.048044972121715546,
-0.03405662253499031,
-0.023243272677063942,
-0.02745097316801548,
0.02294042520225048,
-0.048544906079769135,
-0.03617289289832115,
0.10465076565742493,
-0.010947498492896557,
-0.016683457419276237,
0.02750820852816105,
0.031653717160224915,
-0.0318283885717392,
-0.0027138402219861746,
0.07906104624271393,
0.004616058897227049,
0.045901596546173096,
-0.027229253202676773,
-0.008829299360513687,
-0.060678187757730484,
-0.019603237509727478,
-0.04617282375693321,
-0.019949473440647125,
0.010831808671355247,
0.047329314053058624,
0.09740317612886429,
0.028203798457980156,
0.019960938021540642,
-0.044344913214445114,
-0.10547126829624176,
-0.2826831042766571,
-0.030547700822353363,
0.03822190687060356,
0.03966430202126503,
0.08254015445709229,
-0.03140634298324585,
0.02469281479716301,
-0.04280660301446915,
0.09670422226190567,
-0.07868847250938416,
0.017807090654969215,
0.04094552993774414,
-0.043742600828409195,
-0.020984459668397903,
0.017842134460806847,
-0.007199927233159542,
0.0384686142206192,
-0.018694698810577393,
0.0019552127923816442,
-0.010792017914354801,
-0.006697625387459993,
-0.023643355816602707,
-0.065005362033844,
0.008811450563371181,
0.0567801333963871,
0.011627014726400375,
0.08779419958591461,
0.012695207260549068,
-0.0002642350154928863,
-0.07890353351831436,
-0.04375821724534035,
0.05522384122014046,
0.007024781778454781,
-0.06801801919937134,
-0.051953237503767014,
-0.008841150440275669,
0.0510258786380291,
-0.01837480254471302,
0.05814770981669426,
-0.01988886296749115,
-0.040369048714637756,
0.04544923081994057,
-0.018649039790034294,
-0.004246539436280727,
-0.047038484364748,
-0.0018424278823658824,
-0.015120611526072025,
0.012412204407155514,
0.036840204149484634,
-0.02084839902818203,
0.049517128616571426,
0.025857213884592056,
0.015065313316881657,
-0.05814133957028389,
0.005702218506485224,
0.01128777489066124,
-0.009742691181600094,
0.0528898723423481,
-0.0533534437417984,
0.03654957190155983,
-0.04667080193758011,
0.062451768666505814,
0.028760958462953568,
0.015885626897215843,
0.012028115801513195,
0.005722283851355314,
0.0333964079618454,
0.020799513906240463,
0.06571891903877258,
-0.02753484435379505,
0.03785562887787819,
0.10097299516201019,
-0.0005512047209776938,
0.05450809746980667,
0.10062577575445175,
0.07798144966363907,
-0.022334985435009003,
-0.006484630052000284,
-0.05213918164372444,
0.03572281450033188,
0.07031475752592087,
0.01621917635202408,
0.016914302483201027,
-0.029009316116571426,
0.0020204579923301935,
0.07562416046857834,
0.009383801370859146,
-0.04602211341261864,
-0.0019144391408190131,
0.004611710552126169,
-0.03043336793780327,
-0.019941722974181175,
-0.048138316720724106,
-0.05153808742761612,
-0.006978415418416262,
-0.008991720154881477,
-0.28650256991386414,
0.0008663000771775842,
0.051677364856004715,
0.06794023513793945,
-0.04424065724015236,
0.031136732548475266,
0.05016406998038292,
-0.033294711261987686,
-0.05186246335506439,
0.03673217073082924,
0.012163976207375526,
0.050137922167778015,
0.04213517904281616,
0.07685915380716324,
0.025458764284849167,
0.028171055018901825,
0.03522157296538353,
-0.016023151576519012,
-0.011269351467490196,
-0.007913029752671719,
-0.03529759496450424,
-0.0010991919552907348,
0.1667502522468567,
-0.027250319719314575,
-0.05492302402853966,
0.02200097218155861,
0.015543278306722641,
0.026416432112455368,
0.00811128318309784,
-0.0921190157532692,
0.00509309209883213,
0.0057175601832568645,
0.041936472058296204,
-0.004531382117420435,
0.014234502799808979,
0.02190590649843216,
-0.02122480235993862,
-0.007137051783502102,
-0.05406603217124939,
0.0016102561494335532,
0.022601937875151634,
0.020197955891489983,
-0.031072892248630524,
-0.04868863523006439,
0.044365547597408295,
-0.04494337737560272,
-0.07428859174251556,
0.005306275561451912,
0.0012634694576263428,
-0.0469779372215271,
0.016058167442679405,
0.019043508917093277,
0.001864773454144597,
0.04522246494889259,
0.028915129601955414,
0.07480452954769135,
-0.018028590828180313,
-0.056416481733322144,
-0.01003644522279501,
0.008764310739934444,
0.015544652007520199,
-0.05228142812848091,
-0.03399471193552017,
0.039831917732954025,
0.057308003306388855
] |
PMID:25084 | Effects of pH on beta-hydroxybutyrate transport in rat erythrocytes and thymocytes. | Entry of beta-hydroxybutyrate into erythrocytes and thymocytes is facilitated by a carrier (C), as judged from temperature dependence, saturation kinetics, stereospecificity, competition with lactate and pyruvate, and inhibition by moderate concentrations of methylisobutylxanthine, phloretin, or alpha-cyanocinnamate. We studied the dependence of influx and efflux on internal and external pH and [beta-hydroxybutyrate]. Lowering external pH from 8.0 to 7.3 to 6.6 enhanced influx into erythrocytes by lowering entry Km from 29 to 16 to 10 mM, entry V being independent of external pH. Lowering external pH inhibited efflux. At low external pH, external beta-hydroxybutyrate enhanced efflux slightly. At high external pH, external beta-hydroxybutyrate inhibited efflux. Internal acidification inhibited influx and internal alkalization enhanced influx. Internal beta-hydroxybutyrate (betaHB) enhanced influx more in acidified than alkalized cells. These data are compatible with coupled betaHB-/OH- exchange, betaHB- and OH- competing for influx, C:OH- moving faster than C: betaHB-, empty C being immobile. They are also compatible with coupled betaHB-/H+ copermeation, empty C moving inward faster than H+:C:betaHB-, H+:C being immobile, and C:betaHB- (without H+) being so unstable as not to be formed in significant amounts (relative to C, H+:C, and H+:C:betaHB-). | Effects of pH on beta-hydroxybutyrate transport in rat erythrocytes and thymocytes. Entry of beta-hydroxybutyrate into erythrocytes and thymocytes is facilitated by a carrier (C), as judged from temperature dependence, saturation kinetics, stereospecificity, competition with lactate and pyruvate, and inhibition by moderate concentrations of methylisobutylxanthine, phloretin, or alpha-cyanocinnamate. We studied the dependence of influx and efflux on internal and external pH and [beta-hydroxybutyrate]. Lowering external pH from 8.0 to 7.3 to 6.6 enhanced influx into erythrocytes by lowering entry Km from 29 to 16 to 10 mM, entry V being independent of external pH. Lowering external pH inhibited efflux. At low external pH, external beta-hydroxybutyrate enhanced efflux slightly. At high external pH, external beta-hydroxybutyrate inhibited efflux. Internal acidification inhibited influx and internal alkalization enhanced influx. Internal beta-hydroxybutyrate (betaHB) enhanced influx more in acidified than alkalized cells. These data are compatible with coupled betaHB-/OH- exchange, betaHB- and OH- competing for influx, C:OH- moving faster than C: betaHB-, empty C being immobile. They are also compatible with coupled betaHB-/H+ copermeation, empty C moving inward faster than H+:C:betaHB-, H+:C being immobile, and C:betaHB- (without H+) being so unstable as not to be formed in significant amounts (relative to C, H+:C, and H+:C:betaHB-). | [
-0.042114369571208954,
-0.05481230840086937,
-0.041592683643102646,
0.06883656233549118,
-0.003762997454032302,
0.013632710091769695,
0.04724622890353203,
0.005854337941855192,
-0.06433218717575073,
0.007034252397716045,
0.010730234906077385,
-0.0802646204829216,
-0.008438858203589916,
0.013469677418470383,
-0.03886697068810463,
0.03263627737760544,
-0.001388810109347105,
-0.02558678202331066,
-0.0551137737929821,
-0.01736466772854328,
-0.03733299672603607,
-0.051254745572805405,
-0.002272907178848982,
0.0156406220048666,
0.039864182472229004,
0.025598062202334404,
0.010929759591817856,
0.04184463620185852,
-0.11672759056091309,
-0.20030301809310913,
0.013872075825929642,
-0.005072748754173517,
0.004434000235050917,
-0.009220086969435215,
-0.07142150402069092,
0.044677793979644775,
-0.0009691486484371126,
-0.004683962091803551,
0.008399886079132557,
0.076128289103508,
0.010917788371443748,
0.004146757535636425,
-0.010177561081945896,
0.06520787626504898,
-0.017352048307657242,
-0.01825009472668171,
-0.05742403492331505,
0.01156661193817854,
0.09418855607509613,
0.05131669342517853,
-0.0027039137203246355,
0.030481018126010895,
0.014947451651096344,
0.06265772879123688,
-0.004282146692276001,
0.017441051080822945,
0.044781945645809174,
0.053658947348594666,
0.05098136141896248,
0.031288597732782364,
0.018739130347967148,
0.019151780754327774,
-0.14101733267307281,
0.042394544929265976,
0.0808151513338089,
0.028445210307836533,
-0.058841902762651443,
0.052475009113550186,
0.04531388729810715,
0.04497591033577919,
-0.06173713132739067,
0.025448277592658997,
-0.015350928530097008,
0.045565132051706314,
0.011178397573530674,
-0.05376801639795303,
-0.01612252928316593,
0.004964361432939768,
-0.01639564521610737,
-0.014482378959655762,
0.01583859883248806,
-0.007838611491024494,
-0.014581111259758472,
0.010713235475122929,
0.0361664779484272,
-0.005951453000307083,
0.046979036182165146,
-0.05659753829240799,
-0.05111360549926758,
-0.0163133442401886,
0.006991303525865078,
-0.018464408814907074,
-0.05005171149969101,
0.0650215893983841,
-0.007953912019729614,
0.07285070419311523,
0.02370184287428856,
-0.09854911267757416,
0.03914065286517143,
0.3612361252307892,
-0.018935874104499817,
0.04541797563433647,
-0.013253632001578808,
0.01743539236485958,
0.03853531926870346,
-0.02733730338513851,
0.00637408159673214,
0.0026059781666845083,
-0.03785784915089607,
0.039520662277936935,
0.05006301775574684,
-0.007532353512942791,
0.03778104856610298,
-0.03269914910197258,
-0.02648668922483921,
-0.025723256170749664,
0.015384364873170853,
-0.038713324815034866,
-0.008987785317003727,
0.018648095428943634,
-0.060877006500959396,
0.006685331463813782,
0.03149779886007309,
0.018321827054023743,
0.047270484268665314,
-0.029217848554253578,
-0.01950705796480179,
0.12046259641647339,
0.06713209301233292,
0.012294763699173927,
0.056790437549352646,
0.03405870869755745,
-0.04925087094306946,
0.03575574979186058,
-0.035820234566926956,
-0.012874577194452286,
0.028507888317108154,
-0.023037992417812347,
0.0391814261674881,
0.03679133579134941,
-0.02072792872786522,
-0.038956254720687866,
-0.007750700227916241,
-0.1667214334011078,
-0.07336192578077316,
0.09391419589519501,
0.024908995255827904,
-0.017343934625387192,
-0.02950151637196541,
-0.04092220962047577,
0.008339714258909225,
0.06047750264406204,
0.011574847623705864,
0.02026035636663437,
-0.046190716326236725,
0.01914708875119686,
0.0031201965175569057,
0.010387042537331581,
0.002357572317123413,
-0.007629277650266886,
-0.06543336808681488,
-0.03944039344787598,
-0.016282357275485992,
0.024089861661195755,
-0.012861489318311214,
0.01221394818276167,
-0.018829617649316788,
-0.03817061707377434,
0.03229955956339836,
-0.00642635952681303,
0.013112190179526806,
-0.017198795452713966,
-0.05857902020215988,
-0.07120849192142487,
0.011545293964445591,
0.012523885816335678,
0.011034185066819191,
0.01258180569857359,
0.022207016125321388,
-0.06980038434267044,
0.045023173093795776,
-0.011203193105757236,
-0.032715629786252975,
-0.0246598981320858,
0.09037207812070847,
-0.05783936381340027,
-0.05782465264201164,
-0.01609138958156109,
0.017102595418691635,
0.0519166924059391,
-0.04012499377131462,
-0.00490169133991003,
-0.03488897159695625,
-0.010309138335287571,
-0.024684512987732887,
-0.04430418834090233,
-0.01837882772088051,
-0.010557730682194233,
-0.02742946147918701,
0.015576839447021484,
0.10930373519659042,
-0.018535444512963295,
-0.018777599558234215,
0.06593520194292068,
0.030540820211172104,
0.029124485328793526,
-0.02184896357357502,
0.011255194433033466,
0.0322595052421093,
-0.020292993634939194,
-0.02728007547557354,
-0.006322917062789202,
-0.03310922533273697,
0.02844991721212864,
-0.039487384259700775,
-0.027191707864403725,
0.030298471450805664,
0.033168889582157135,
0.0919172391295433,
-0.04119143635034561,
0.05923394486308098,
-0.07773095369338989,
-0.049180302768945694,
-0.2807774841785431,
-0.031874436885118484,
0.01715199276804924,
0.014009007252752781,
0.06014774739742279,
-0.05478173866868019,
0.058210164308547974,
-0.021133024245500565,
-0.011957474052906036,
-0.04729660600423813,
0.04944156855344772,
0.040058352053165436,
-0.036955833435058594,
-0.07071170955896378,
0.007717089261859655,
-0.012562002055346966,
0.048337675631046295,
0.012955901212990284,
0.08992161601781845,
-0.007813769392669201,
0.07123272120952606,
-0.04940366744995117,
0.004674029536545277,
0.04107627272605896,
0.05616156384348869,
0.01265679020434618,
0.08027826249599457,
-0.022605011239647865,
0.006735675968229771,
-0.03301145136356354,
0.028543200343847275,
0.05249466374516487,
0.02941390872001648,
-0.09904536604881287,
-0.04180896654725075,
-0.052154701203107834,
0.05882519111037254,
-0.009594819508492947,
0.009151356294751167,
-0.009342384524643421,
-0.07196494191884995,
0.002102543832734227,
-0.050386685878038406,
-0.0684349313378334,
-0.024439910426735878,
0.0030702627263963223,
0.027627017349004745,
0.04908985644578934,
-0.03980255499482155,
-0.01790776289999485,
0.026634883135557175,
-0.007994472049176693,
0.024362225085496902,
-0.05201542377471924,
0.0576082319021225,
-0.017946239560842514,
-0.014501119032502174,
0.022868391126394272,
-0.06696253269910812,
-0.017035260796546936,
-0.06750314682722092,
0.0033075185492634773,
-0.021864835172891617,
0.0234083142131567,
0.006814077030867338,
-0.039357710629701614,
0.07227712124586105,
0.025895437225699425,
0.02288735844194889,
0.055523913353681564,
0.03319697454571724,
0.11081352084875107,
-0.014670148491859436,
0.011825698427855968,
0.0042698015458881855,
0.023239726200699806,
-0.06074323505163193,
-0.037754837423563004,
-0.07709797471761703,
0.010339154861867428,
-0.002819445449858904,
-0.06538107246160507,
0.09213156998157501,
-0.05835163965821266,
-0.01568158157169819,
0.039725713431835175,
0.03023678809404373,
-0.00006458604912040755,
0.001514269271865487,
0.016859950497746468,
-0.06850753724575043,
-0.019893957301974297,
-0.01748628169298172,
-0.02569875679910183,
0.04833190515637398,
0.0010728917550295591,
-0.2546262741088867,
0.019791997969150543,
0.023817816749215126,
-0.029738901183009148,
-0.03527677804231644,
0.027331417426466942,
0.026027502492070198,
-0.030058130621910095,
-0.04954272508621216,
0.016771158203482628,
0.04194042831659317,
0.002834978047758341,
0.06477629393339157,
0.07320012897253036,
-0.030915845185518265,
0.020437069237232208,
0.062422335147857666,
0.025075847283005714,
0.02588346228003502,
-0.04743294417858124,
-0.013553529046475887,
-0.005644000135362148,
0.12650536000728607,
-0.020075328648090363,
-0.016521340236067772,
0.033606287091970444,
0.011500595137476921,
0.04958760738372803,
-0.04939107596874237,
-0.015217968262732029,
-0.02373727783560753,
0.0581137053668499,
0.06580328941345215,
-0.025449251756072044,
0.0321611724793911,
-0.0017573062796145678,
-0.007637363392859697,
-0.035105034708976746,
-0.0070467605255544186,
0.011678529903292656,
0.016393296420574188,
0.032597292214632034,
-0.03774969279766083,
0.011842092499136925,
0.07354886829853058,
-0.040174927562475204,
-0.0435548759996891,
-0.019584545865654945,
0.029828350991010666,
-0.009175354614853859,
-0.006795794703066349,
-0.05743857100605965,
0.036766864359378815,
0.05633595585823059,
-0.012267090380191803,
-0.006992939859628677,
-0.013495131395757198,
-0.035837750881910324,
-0.019901299849152565,
-0.015903014689683914,
0.031917113810777664,
-0.02443283423781395,
-0.05521455034613609,
0.07359620183706284,
0.08659236133098602
] |
PMID:25086 | The kinetics and equilibria of squirrel-fish hemoglobin. A Root effect hemoglobin complicated by large subunit heterogeneity. | The functional properties of squirrel-fish hemoglobin have been measured by studying ligand binding equilibria and kinetics. The results show that squirrel-fish hemoglobin has a Root effect with a corresponding stabilization of the low affinity state. The properties of this state are pH dependent even in the absence of cooperativity. The effect of ATP shifts the overall ligant affinity towards the low affinity state and is characteristic of the allosteric effect caused by organic phosphates. Under pH and ATP conditions favoring the low affinity conformational state, a 10-fold difference in the binding kinetics of carbon monoxide to the alpha and beta subunits is observed. | The kinetics and equilibria of squirrel-fish hemoglobin. A Root effect hemoglobin complicated by large subunit heterogeneity. The functional properties of squirrel-fish hemoglobin have been measured by studying ligand binding equilibria and kinetics. The results show that squirrel-fish hemoglobin has a Root effect with a corresponding stabilization of the low affinity state. The properties of this state are pH dependent even in the absence of cooperativity. The effect of ATP shifts the overall ligant affinity towards the low affinity state and is characteristic of the allosteric effect caused by organic phosphates. Under pH and ATP conditions favoring the low affinity conformational state, a 10-fold difference in the binding kinetics of carbon monoxide to the alpha and beta subunits is observed. | [
-0.09752111881971359,
0.06083701178431511,
-0.036379579454660416,
-0.00504325283691287,
-0.003209021408110857,
-0.0141304861754179,
0.07911315560340881,
0.009196463972330093,
-0.07985764741897583,
0.018254496157169342,
0.03385181352496147,
-0.0398508720099926,
0.003976820968091488,
0.033782169222831726,
-0.049550287425518036,
-0.04423994943499565,
0.011380864307284355,
0.0020346487872302532,
-0.05869229510426521,
0.013371773064136505,
-0.002622713800519705,
-0.053816910833120346,
0.005032714921981096,
-0.018269389867782593,
0.028965389356017113,
0.09808661788702011,
-0.011267050169408321,
0.04837393760681152,
-0.05025763437151909,
-0.210953027009964,
0.012188198044896126,
-0.004311069380491972,
0.013063442893326283,
-0.0078017194755375385,
0.004005920607596636,
0.004455231130123138,
-0.04418184235692024,
-0.01531991083174944,
-0.06585560739040375,
0.031925518065690994,
0.058182813227176666,
0.033814746886491776,
0.023929914459586143,
0.06941435486078262,
-0.011291900649666786,
-0.013940212316811085,
-0.05546010658144951,
0.013520510867238045,
0.04343100264668465,
0.04550563916563988,
-0.027893254533410072,
0.008908172138035297,
-0.012644429691135883,
0.001520303194411099,
-0.00659177266061306,
0.06921574473381042,
0.05355658382177353,
0.0763578787446022,
0.006390982307493687,
-0.011603373102843761,
0.04490654915571213,
0.04033605754375458,
-0.10177478194236755,
0.078481525182724,
0.10273309797048569,
0.027420274913311005,
-0.011870562098920345,
-0.0370195247232914,
0.06856022775173187,
0.044035278260707855,
-0.01961975358426571,
0.04522048309445381,
0.025275569409132004,
-0.03068484552204609,
0.02531065233051777,
-0.012789351865649223,
-0.021275892853736877,
0.01954161748290062,
0.044926874339580536,
0.028606262058019638,
-0.06874965876340866,
-0.00098831404466182,
0.009030626155436039,
-0.0005716746090911329,
-0.006922855973243713,
0.02348886802792549,
0.03310558199882507,
-0.08222270011901855,
-0.02178359031677246,
0.04769403114914894,
0.0102677121758461,
-0.052165549248456955,
0.002621239982545376,
-0.031325239688158035,
-0.0796484649181366,
0.05316254124045372,
-0.006306393537670374,
-0.020425520837306976,
-0.016152458265423775,
0.38023579120635986,
-0.001652334351092577,
0.08382299542427063,
-0.03214575722813606,
-0.04414376616477966,
0.05925478786230087,
-0.05304214358329773,
-0.011037496849894524,
0.0017469944432377815,
0.017030009999871254,
-0.0012719371588900685,
0.03697572648525238,
-0.04207272827625275,
0.032243162393569946,
-0.06896562874317169,
-0.024905284866690636,
0.017662324011325836,
-0.020197944715619087,
-0.002528606681153178,
-0.029215089976787567,
0.016430644318461418,
-0.050459276884794235,
0.018400035798549652,
0.0026567240711301565,
0.03109993226826191,
0.04436078295111656,
0.04235002025961876,
-0.026656856760382652,
0.08981917798519135,
0.04650318622589111,
0.040100257843732834,
0.07190852612257004,
0.008571633137762547,
-0.012169093824923038,
-0.021602945402264595,
-0.017162011936306953,
0.02609851025044918,
0.04948447644710541,
0.011879464611411095,
0.018468935042619705,
0.0020901598036289215,
-0.002028388436883688,
-0.036129314452409744,
-0.043502919375896454,
-0.07624195516109467,
-0.058636438101530075,
0.02016184665262699,
0.020821141079068184,
0.013560682535171509,
-0.03962574154138565,
0.0036352789029479027,
-0.0668565183877945,
0.018386632204055786,
-0.035773538053035736,
0.0185390692204237,
-0.0337194949388504,
0.031772952526807785,
-0.011962722055613995,
0.00933487992733717,
0.022834794595837593,
-0.04873499646782875,
-0.15123315155506134,
0.006354792509227991,
0.0006460511940531433,
-0.0031238507945090532,
-0.009557738900184631,
0.008586975745856762,
0.00874681118875742,
-0.011960631236433983,
-0.003287835977971554,
0.0026843505911529064,
-0.024668771773576736,
0.0006061705062165856,
0.038050636649131775,
-0.04344096779823303,
-0.0010182886617258191,
0.042619138956069946,
0.00931631401181221,
0.03243232145905495,
-0.0234350748360157,
-0.007452079560607672,
0.021876120939850807,
0.03908518701791763,
-0.04993237182497978,
0.02326364256441593,
0.027532782405614853,
0.0188625268638134,
-0.09590419381856918,
0.028355607762932777,
-0.038533732295036316,
0.05766895413398743,
-0.03862140327692032,
-0.03759761527180672,
-0.05631010979413986,
0.0667961984872818,
-0.022219594568014145,
-0.031971290707588196,
-0.01535103004425764,
-0.047808486968278885,
-0.033046308904886246,
0.014673651196062565,
0.08342181891202927,
-0.028537515550851822,
-0.024043215438723564,
0.04407911002635956,
0.03962479904294014,
0.0318186953663826,
0.01928696781396866,
0.01822991855442524,
0.01907959394156933,
-0.02468271739780903,
-0.05671798437833786,
0.0075879935175180435,
-0.08196014165878296,
-0.006638780701905489,
-0.07027994841337204,
-0.04567243158817291,
-0.0002993358066305518,
0.052730824798345566,
0.06362377107143402,
0.021701257675886154,
-0.008053611032664776,
-0.08792992681264877,
-0.05035507306456566,
-0.32029661536216736,
-0.024635642766952515,
-0.012621634639799595,
-0.011905341409146786,
0.045214924961328506,
-0.013527480885386467,
0.012154159136116505,
0.012842286378145218,
0.005454282741993666,
-0.0421549491584301,
0.012622123584151268,
0.06835518032312393,
-0.012496525421738625,
-0.06398095190525055,
-0.001572658191435039,
0.003943983465433121,
0.06418635696172714,
-0.003947888966649771,
-0.014421818777918816,
0.07053836435079575,
0.00832886528223753,
0.011866766028106213,
-0.04620664566755295,
0.0022401995956897736,
0.051882486790418625,
0.009700815193355083,
0.09600389748811722,
-0.01938210241496563,
-0.03723717853426933,
0.03328640013933182,
0.004828409757465124,
0.044467341154813766,
-0.0005246679065749049,
-0.05000372603535652,
-0.028938746079802513,
-0.020992601290345192,
-0.0119888074696064,
-0.03368711844086647,
0.036153532564640045,
-0.013680239208042622,
0.005256825592368841,
0.016487568616867065,
0.018615562468767166,
-0.0448959581553936,
-0.010816945694386959,
0.027120007202029228,
0.025653887540102005,
0.006233433727174997,
-0.013636630028486252,
-0.05348651111125946,
0.07047340273857117,
-0.051017094403505325,
0.0072069596499204636,
-0.024310002103447914,
-0.011707738973200321,
-0.006101432256400585,
-0.002143073594197631,
0.06376847624778748,
-0.07237839698791504,
-0.008705591782927513,
0.0038661931175738573,
0.01263668853789568,
-0.012423082254827023,
0.06489639729261398,
0.023254429921507835,
-0.004788795951753855,
0.025426262989640236,
-0.012009718455374241,
0.042169731110334396,
-0.006279707420617342,
0.024709012359380722,
0.06987638771533966,
-0.05281434208154678,
0.03379754349589348,
0.004307539667934179,
0.03986375033855438,
-0.058770086616277695,
0.0015618561301380396,
0.0027525080367922783,
-0.014859842136502266,
0.0604989267885685,
0.03961803764104843,
0.056942205876111984,
-0.028810247778892517,
-0.01827366091310978,
0.038181137293577194,
0.04688958823680878,
-0.026426034048199654,
0.038344115018844604,
-0.000046339180698851123,
0.0014598522102460265,
-0.04318951442837715,
-0.010559291578829288,
-0.04036591947078705,
0.01329756435006857,
0.07724122703075409,
-0.27962881326675415,
-0.003350422717630863,
0.003102481598034501,
0.04277953505516052,
-0.012993081472814083,
-0.009228736162185669,
0.05104732885956764,
-0.0836859941482544,
-0.05612952634692192,
0.05925621837377548,
-0.005812230985611677,
0.06831265985965729,
0.02669743075966835,
0.03375212103128433,
0.02050810120999813,
-0.010569925419986248,
0.028836702927947044,
-0.10137143731117249,
-0.018858641386032104,
-0.05504991486668587,
-0.017124731093645096,
0.018887436017394066,
0.17808708548545837,
-0.035825930535793304,
-0.01329009048640728,
0.052563201636075974,
0.016814546659588814,
0.034631554037332535,
-0.06785888969898224,
-0.014180424623191357,
0.009174096398055553,
0.036953866481781006,
0.07959290593862534,
-0.06847521662712097,
0.029596775770187378,
0.05657290294766426,
-0.02709619514644146,
-0.06679703295230865,
-0.008384761400520802,
0.011252461932599545,
-0.008623916655778885,
0.017175745218992233,
-0.02929268218576908,
-0.013069038279354572,
0.06122833862900734,
-0.03140626102685928,
-0.07043550163507462,
-0.004130161367356777,
-0.013649007305502892,
0.013165834359824657,
-0.019047528505325317,
0.0030479258857667446,
0.005567591637372971,
-0.0004586192953865975,
0.011496683582663536,
0.0031465794891119003,
-0.004232700448483229,
-0.030191516503691673,
-0.03751612454652786,
-0.009992546401917934,
0.026320183649659157,
0.016714056953787804,
-0.05997339263558388,
0.06506536155939102,
0.04021043702960014
] |
PMID:25087 | Circular dichroism of metallothioneins. A structural approach. | A comprehensive study on circular dichroism of metallothioneins containing Zn, Cd and Cu was carried out. The contributions of the metals, the sulphur and the polypeptide chain to the observed Cotton effects was shown. From the pH dependency of the extrinsic Cotton effects which are due to the metal-thiolate chromophore the stability of the metal clusters was found to decrease in the order Cu greater than Cd greater than Zn. The pH values corresponding to the dissociation of half of the bound metal ions are 0.44 for Cu-thionein, 3.05 for Cd-thionein and 4.6 for Zn-thionein. The extrinsic Cotton effects of Cd, Zn-thioneins of varying Cd to Zn ratio could be simulated using the difference circular dichroic spectra of Cd-thionein (bands at 227, 242.5 and 262 nm), Zn-thionein (bands at 225 and 244 nm) and the circular dichroic spectrum of cysteine-thionein (band at 200 nm, shoulder at 225 nm). Since during the dissociation of the metals the circular dichroic spectra exhibited changes only in amplitude and not in shape we can conclude that the dissociation of the metal ions involves the complete sequential degradation of metal clusters. In the near-ultraviolet region the metal-free proteins show only Cotton effects attributable to a disulphide chromophore. Thus Cotton bands are observed for cystine-thionein at 282.5 and 260 nm. From the intrinsic circular dichroism of Cd- and Zn-thionein (negative Cotton effect at 200 nm, shoulder at 225 nm) it follows that the protein conformation consists of less than 5% helical or pleated sheet structure and therefore has to be classified as unordered structure or "fixed" random coil | Circular dichroism of metallothioneins. A structural approach. A comprehensive study on circular dichroism of metallothioneins containing Zn, Cd and Cu was carried out. The contributions of the metals, the sulphur and the polypeptide chain to the observed Cotton effects was shown. From the pH dependency of the extrinsic Cotton effects which are due to the metal-thiolate chromophore the stability of the metal clusters was found to decrease in the order Cu greater than Cd greater than Zn. The pH values corresponding to the dissociation of half of the bound metal ions are 0.44 for Cu-thionein, 3.05 for Cd-thionein and 4.6 for Zn-thionein. The extrinsic Cotton effects of Cd, Zn-thioneins of varying Cd to Zn ratio could be simulated using the difference circular dichroic spectra of Cd-thionein (bands at 227, 242.5 and 262 nm), Zn-thionein (bands at 225 and 244 nm) and the circular dichroic spectrum of cysteine-thionein (band at 200 nm, shoulder at 225 nm). Since during the dissociation of the metals the circular dichroic spectra exhibited changes only in amplitude and not in shape we can conclude that the dissociation of the metal ions involves the complete sequential degradation of metal clusters. In the near-ultraviolet region the metal-free proteins show only Cotton effects attributable to a disulphide chromophore. Thus Cotton bands are observed for cystine-thionein at 282.5 and 260 nm. From the intrinsic circular dichroism of Cd- and Zn-thionein (negative Cotton effect at 200 nm, shoulder at 225 nm) it follows that the protein conformation consists of less than 5% helical or pleated sheet structure and therefore has to be classified as unordered structure or "fixed" random coil | [
-0.09478693455457687,
0.015417976304888725,
-0.026002805680036545,
0.04673103988170624,
0.07604503631591797,
-0.025416601449251175,
0.026289720088243484,
0.005696726497262716,
-0.05595078319311142,
0.004878079518675804,
0.04325505718588829,
-0.027445219457149506,
0.023661674931645393,
0.023124832659959793,
-0.004907591268420219,
-0.03198299556970596,
0.03231847658753395,
-0.009476200677454472,
-0.06025613099336624,
0.01149878092110157,
-0.008855711668729782,
-0.010133929550647736,
0.02081916481256485,
-0.025101609528064728,
0.038618091493844986,
0.05268409103155136,
-0.021339111030101776,
0.05433843284845352,
-0.0883859172463417,
-0.2166779488325119,
0.0009862176375463605,
0.06488695740699768,
-0.01602919213473797,
-0.0020474984776228666,
-0.02757459133863449,
-0.04271702468395233,
0.01309762429445982,
-0.032230064272880554,
-0.004349065944552422,
-0.017533274367451668,
0.042283326387405396,
0.05659223347902298,
-0.026953816413879395,
-0.011676390655338764,
-0.0071994345635175705,
-0.03443238511681557,
-0.019348205998539925,
0.0018877736292779446,
0.05266686901450157,
0.040503453463315964,
0.03605206310749054,
-0.018297500908374786,
-0.01284739188849926,
0.0717344582080841,
0.0037938302848488092,
0.06870377063751221,
0.02159315161406994,
0.09295359253883362,
-0.03609538823366165,
0.025695184245705605,
0.05647282674908638,
-0.029306309297680855,
-0.08867362141609192,
0.06696503609418869,
0.08099044859409332,
0.05238600820302963,
0.0007422620547004044,
-0.048004601150751114,
0.035667553544044495,
0.051217466592788696,
-0.02382027730345726,
0.08220776170492172,
-0.023403774946928024,
0.002720322459936142,
0.006022470537573099,
-0.03250391408801079,
-0.016499193385243416,
-0.05317419022321701,
0.023013431578874588,
-0.009215127676725388,
0.006691453047096729,
0.040550269186496735,
0.04630700498819351,
-0.0077822222374379635,
-0.0010582796530798078,
0.03879545256495476,
-0.03914318606257439,
-0.026419145986437798,
-0.013130946084856987,
0.004279559478163719,
-0.037668365985155106,
-0.06918691098690033,
-0.02980123646557331,
-0.011041244491934776,
-0.06693760305643082,
0.011124823242425919,
0.00033961032750084996,
-0.031129879876971245,
-0.02586769126355648,
0.38789716362953186,
-0.03425498306751251,
-0.006403953768312931,
-0.042658817023038864,
-0.056667063385248184,
0.01011798158288002,
-0.044056229293346405,
-0.03356310725212097,
-0.05476536974310875,
-0.008426833897829056,
0.061836130917072296,
0.04537075757980347,
-0.013625857420265675,
-0.06956003606319427,
-0.077986940741539,
0.0018783652922138572,
-0.03569205477833748,
0.004150377586483955,
0.020848840475082397,
-0.011167813092470169,
-0.012996512465178967,
-0.04997425526380539,
0.04635966196656227,
0.01966221071779728,
0.007277430035173893,
-0.008377471007406712,
-0.03397651016712189,
-0.029921531677246094,
0.07674497365951538,
0.032953813672065735,
0.035376306623220444,
0.025095980614423752,
0.015140973962843418,
0.0022599438671022654,
0.01704012043774128,
-0.02603153884410858,
0.006777465343475342,
0.04358533024787903,
-0.04339626803994179,
0.04170793294906616,
0.010121701285243034,
-0.04423999413847923,
-0.07844255864620209,
-0.07081515341997147,
-0.054166100919246674,
-0.03169139102101326,
0.0302883293479681,
0.03981899097561836,
0.012562217190861702,
0.005705829244107008,
0.014742535538971424,
0.0053698113188147545,
0.05520351231098175,
-0.05364947393536568,
0.01862606778740883,
-0.003103013150393963,
0.049219418317079544,
-0.02368185482919216,
-0.019793258979916573,
0.030966799706220627,
-0.0073138135485351086,
-0.06200898066163063,
0.01808316633105278,
0.017355702817440033,
0.09158258140087128,
0.039488792419433594,
-0.05254645645618439,
-0.010913335718214512,
0.030562683939933777,
0.019012080505490303,
-0.058323126286268234,
0.019268473610281944,
0.03092055581510067,
-0.03229370713233948,
0.0011234050616621971,
0.0014818162890151143,
0.0257276501506567,
0.012844288721680641,
-0.019443891942501068,
0.021700121462345123,
0.0241593886166811,
0.03831963986158371,
0.04450631141662598,
-0.015046803280711174,
0.05100918188691139,
0.058661412447690964,
0.017712783068418503,
-0.08329037576913834,
0.05280933156609535,
0.00940793938934803,
0.032726772129535675,
-0.048173826187849045,
-0.00935354270040989,
-0.05586402118206024,
0.04223481938242912,
-0.04467121511697769,
-0.08111473172903061,
-0.04072749987244606,
0.015188966877758503,
-0.005211431998759508,
-0.022366568446159363,
0.1112416461110115,
-0.02082996815443039,
-0.031001387163996696,
0.05076441541314125,
-0.0017084742430597544,
0.05329561233520508,
0.05243176594376564,
0.018721962347626686,
0.03065400756895542,
-0.033842142671346664,
-0.031451210379600525,
0.04905340075492859,
-0.05429809167981148,
-0.01443575881421566,
-0.05835294723510742,
0.02477267198264599,
0.03114989772439003,
0.05233350396156311,
0.0041271899826824665,
0.059493955224752426,
-0.005839133635163307,
-0.059596821665763855,
-0.09245674312114716,
-0.30596253275871277,
-0.027973409742116928,
0.0301393773406744,
-0.031265031546354294,
0.09453946352005005,
-0.046060726046562195,
-0.047655098140239716,
0.01630018837749958,
0.039197586476802826,
-0.06429439038038254,
0.016435351222753525,
0.1303575187921524,
-0.04699733853340149,
-0.09017197042703629,
-0.025195235386490822,
-0.003175916615873575,
0.04059276729822159,
-0.04262678325176239,
0.017201203852891922,
0.05379335954785347,
-0.039296574890613556,
-0.03593514859676361,
-0.0039329552091658115,
0.019313324242830276,
0.04900655522942543,
0.009090309962630272,
0.11685872077941895,
0.014606447890400887,
-0.05188143625855446,
0.03810304403305054,
-0.053697604686021805,
0.04857897758483887,
-0.008980141021311283,
-0.013247345574200153,
-0.04917006194591522,
0.0205670278519392,
0.007408298086374998,
-0.02266938053071499,
-0.02242082916200161,
-0.024429133161902428,
0.003672007005661726,
0.02774408459663391,
0.03860155865550041,
-0.05982816219329834,
-0.019609276205301285,
0.0139541020616889,
0.021609125658869743,
-0.01830173283815384,
0.02861678972840309,
0.009997116401791573,
0.02393372170627117,
-0.022671693935990334,
0.0862959697842598,
0.021564053371548653,
0.01377304270863533,
0.009648443199694157,
-0.01097392849624157,
0.018982982262969017,
-0.017138535156846046,
-0.01903590001165867,
-0.01650901325047016,
-0.04178565740585327,
-0.00031612763996236026,
0.024036360904574394,
0.017044691368937492,
-0.03998718410730362,
0.04671075567603111,
-0.05377357453107834,
0.077951580286026,
-0.052243202924728394,
-0.029473870992660522,
0.09891925007104874,
-0.021459801122546196,
0.005388231016695499,
0.0038738823495805264,
0.0320577472448349,
-0.038658708333969116,
0.006602628156542778,
-0.027598701417446136,
0.057189226150512695,
0.0393693633377552,
0.025213679298758507,
0.10883817821741104,
-0.08204544335603714,
-0.010573568753898144,
0.037065908312797546,
-0.008884240873157978,
-0.025476446375250816,
0.06801582872867584,
0.014736163429915905,
0.028998158872127533,
-0.019977843388915062,
-0.024541055783629417,
0.006773232948035002,
0.027201995253562927,
0.0462198480963707,
-0.2651441693305969,
-0.02681005746126175,
0.04002233222126961,
0.06643620878458023,
-0.03466341644525528,
0.056540027260780334,
0.06144523620605469,
-0.0527958944439888,
-0.02659757249057293,
0.0534164123237133,
-0.04094915837049484,
0.04004335403442383,
0.04636051505804062,
-0.059711139649152756,
-0.003010059939697385,
-0.004375880118459463,
0.029419491067528725,
-0.057597748935222626,
-0.024069828912615776,
0.02188139595091343,
-0.013042396865785122,
-0.026224754750728607,
0.15298259258270264,
-0.01246780063956976,
0.002264507580548525,
0.05428599193692207,
-0.006954234559088945,
0.032702088356018066,
-0.047592081129550934,
0.04453786090016365,
0.005368615034967661,
-0.0004386163782328367,
0.036863528192043304,
-0.03254920244216919,
0.0011192579986527562,
0.007623473647981882,
-0.016333753243088722,
-0.05330279469490051,
-0.008123236708343029,
-0.014218258671462536,
0.0357540138065815,
-0.03438577800989151,
-0.07901214808225632,
-0.05461675673723221,
0.03454199060797691,
-0.02511158213019371,
-0.03756435960531235,
-0.0051273442804813385,
0.016078025102615356,
0.0007394349086098373,
-0.018288854509592056,
0.005382324568927288,
-0.017402108758687973,
0.004992404021322727,
-0.0409490168094635,
0.013643448241055012,
-0.020056122913956642,
-0.0015996332513168454,
-0.004653290845453739,
0.008737005293369293,
-0.0019769922364503145,
-0.04320588335394859,
-0.03645063936710358,
0.03488902375102043,
0.028753945603966713
] |
PMID:25088 | Changes in structure and hydrophobic surface properties of beta-lactoglobulin determined by partition in aqueous two-phase polymeric systems. | The non-polar surface properties of beta-lactoglobulin and especially its interaction with poly(ethylene glycol)-bound palmitate has been studied as a function of pH, temperature and protein concentration. The maximum interaction between beta-lactoglobulin and polymer-bound palmitate occurs at pH 4.3 and pH 7.8. The change in conformation of beta-lactoglobulin around pH 7.5 seems to involve exposure of apolar amino acids to the solvent which results in an increased affinity for hydrocarbons. This is contrary to the situation at pH 4.8--6.0 where the corresponding change in conformation does not affect the protein-hydrocarbon interaction. The results suggest that partition studies in an aqueous two-phase system is a very useful tool to detect changes in conformation and aggregation and to characterize the corresponding hydrophobic surface properties of a protein. | Changes in structure and hydrophobic surface properties of beta-lactoglobulin determined by partition in aqueous two-phase polymeric systems. The non-polar surface properties of beta-lactoglobulin and especially its interaction with poly(ethylene glycol)-bound palmitate has been studied as a function of pH, temperature and protein concentration. The maximum interaction between beta-lactoglobulin and polymer-bound palmitate occurs at pH 4.3 and pH 7.8. The change in conformation of beta-lactoglobulin around pH 7.5 seems to involve exposure of apolar amino acids to the solvent which results in an increased affinity for hydrocarbons. This is contrary to the situation at pH 4.8--6.0 where the corresponding change in conformation does not affect the protein-hydrocarbon interaction. The results suggest that partition studies in an aqueous two-phase system is a very useful tool to detect changes in conformation and aggregation and to characterize the corresponding hydrophobic surface properties of a protein. | [
-0.09048913419246674,
-0.015350012108683586,
-0.019089998677372932,
0.008411798626184464,
0.0017240609740838408,
-0.012158088386058807,
0.07036522030830383,
0.02267218753695488,
-0.043743494898080826,
-0.01938379928469658,
-0.009374292567372322,
-0.07999573647975922,
-0.023095065727829933,
0.02973577193915844,
0.006965108215808868,
-0.0029791451524943113,
0.02320709079504013,
-0.021432718262076378,
-0.021143687888979912,
-0.026115434244275093,
0.03680124506354332,
-0.07662245631217957,
-0.023820431903004646,
-0.0391080416738987,
0.06188524141907692,
0.05682482197880745,
0.008525372482836246,
0.06023501232266426,
-0.08944645524024963,
-0.2640392482280731,
0.02929714322090149,
-0.013644182123243809,
0.021162832155823708,
-0.005765147507190704,
-0.014602490700781345,
0.020075684413313866,
0.03977927193045616,
0.00947973970323801,
-0.008803828619420528,
0.00908383447676897,
0.051093690097332,
0.02288295328617096,
-0.01570110209286213,
-0.00472214724868536,
0.06957702338695526,
-0.02930363267660141,
-0.047108475118875504,
0.040036391466856,
0.0617053359746933,
0.022544728592038155,
-0.058732789009809494,
-0.00531129352748394,
0.006401948630809784,
0.033044010400772095,
0.007890687324106693,
0.05927836894989014,
-0.02465631626546383,
0.054410383105278015,
0.015360856428742409,
0.00528689892962575,
-0.011374561116099358,
-0.018307436257600784,
-0.11875756084918976,
0.15119390189647675,
0.03349926695227623,
0.08388358354568481,
-0.0205583143979311,
0.013039231300354004,
0.04530094563961029,
0.046020764857530594,
-0.04988003522157669,
0.047057364135980606,
0.018372701480984688,
0.0007760419393889606,
0.02788514457643032,
-0.02776961401104927,
-0.053387969732284546,
0.026019064709544182,
0.03816933184862137,
0.012141052633523941,
-0.003129935823380947,
0.000009473164936935063,
0.05577531084418297,
-0.017778201028704643,
-0.07010140269994736,
0.0018571631517261267,
0.02235371246933937,
-0.0352819450199604,
-0.009931141510605812,
0.03665662556886673,
0.0435292087495327,
-0.04465767741203308,
0.00701089296489954,
-0.0003990782133769244,
-0.09171754866838455,
0.05062193423509598,
0.001973788719624281,
-0.06610482931137085,
0.059272948652505875,
0.40068042278289795,
-0.0753837376832962,
0.012893310748040676,
-0.013631374575197697,
-0.018162978813052177,
0.028273722156882286,
-0.01586146466434002,
0.021975193172693253,
0.008700557053089142,
0.05328522250056267,
0.02549814246594906,
-0.0025983855593949556,
-0.0043628322891891,
-0.022456103935837746,
-0.04070692136883736,
-0.002912403317168355,
-0.030213158577680588,
0.004498043563216925,
-0.023372037336230278,
-0.010885012336075306,
-0.019142156466841698,
-0.029999960213899612,
0.004916892386972904,
0.014936722815036774,
-0.008609890006482601,
0.045554809272289276,
0.0141106266528368,
0.0039464375004172325,
0.04740782454609871,
0.058431319892406464,
0.007693652994930744,
0.027640679851174355,
0.015092278830707073,
-0.02907044067978859,
-0.03177274763584137,
0.03221563249826431,
-0.02731592394411564,
0.0058647021651268005,
0.01664559543132782,
0.037201520055532455,
0.020746026188135147,
-0.024140387773513794,
-0.03457272797822952,
0.0023105295840650797,
-0.08551765978336334,
-0.024577904492616653,
0.05318878963589668,
-0.011468683369457722,
0.009385819546878338,
-0.014412829652428627,
0.037437744438648224,
-0.010279666632413864,
0.049795929342508316,
-0.025261588394641876,
0.029180360957980156,
0.05264779180288315,
-0.019407756626605988,
0.03711879253387451,
0.0004656005185097456,
0.0003950855752918869,
-0.04043825715780258,
-0.07619179785251617,
-0.011792331002652645,
0.003241464961320162,
0.033249229192733765,
0.0011991758365184069,
-0.03081124648451805,
-0.02328900247812271,
-0.0768808126449585,
0.0341372936964035,
-0.012235918082296848,
0.044246457517147064,
0.00991446990519762,
0.012156776152551174,
-0.05443872511386871,
-0.01919287070631981,
0.05938330665230751,
0.012031284160912037,
-0.0027100008446723223,
0.02145165391266346,
-0.039942920207977295,
-0.006525110919028521,
0.030671797692775726,
-0.026402752846479416,
0.022340072318911552,
0.04947659373283386,
-0.036243464797735214,
-0.07748211920261383,
0.00327285542152822,
-0.0021214052103459835,
0.032879892736673355,
-0.04957939684391022,
-0.014002636075019836,
-0.07458118349313736,
0.07790809869766235,
0.02852497436106205,
0.029645387083292007,
-0.019739648327231407,
-0.007445186376571655,
-0.0021383098792284727,
0.00496652303263545,
0.1325431764125824,
0.01155210379511118,
-0.02506600134074688,
-0.02271673083305359,
0.08543878048658371,
0.07012584060430527,
0.02037542127072811,
-0.0028030865360051394,
0.02099553681910038,
0.012297888286411762,
-0.057719986885786057,
-0.006607697810977697,
-0.13803169131278992,
-0.0688992515206337,
-0.0389251783490181,
0.03643055260181427,
0.04106418788433075,
-0.005852370988577604,
0.025489522144198418,
0.030170200392603874,
0.06963753700256348,
-0.003903477219864726,
-0.0619751438498497,
-0.27220672369003296,
-0.0027617488522082567,
-0.04539768770337105,
-0.011070333421230316,
0.06646689772605896,
-0.06906572729349136,
0.010190065950155258,
0.024578243494033813,
0.028277471661567688,
-0.04455656558275223,
0.047551389783620834,
0.03082333505153656,
-0.03982112556695938,
-0.026212114840745926,
-0.014025537297129631,
-0.00827291514724493,
0.05982448160648346,
-0.039961814880371094,
0.03143187612295151,
0.027684470638632774,
0.003107290016487241,
0.019598035141825676,
0.01838504895567894,
0.02887415699660778,
0.07768483459949493,
-0.012255052104592323,
0.1084105521440506,
-0.024202728644013405,
-0.07199636101722717,
0.003180605126544833,
0.006326147820800543,
0.04738004878163338,
-0.045544177293777466,
-0.05358128994703293,
-0.0051739150658249855,
0.019225316122174263,
-0.000701709883287549,
-0.014358687214553356,
-0.0008689241949468851,
0.02033142000436783,
-0.03430046886205673,
0.027680514380335808,
-0.00000990733224170981,
-0.06100725010037422,
-0.04561970382928848,
-0.009583955630660057,
0.03224294260144234,
0.04353903979063034,
-0.041627902537584305,
0.012079230509698391,
0.023016108199954033,
-0.005199849139899015,
0.009007534012198448,
-0.032507214695215225,
-0.0022110489662736654,
0.015107729472219944,
0.01676897518336773,
-0.022762352600693703,
-0.05386205017566681,
0.0391462966799736,
0.030992086976766586,
-0.015417028218507767,
-0.006273919716477394,
0.04499034956097603,
0.015984123572707176,
0.009708949364721775,
0.014258398674428463,
-0.0589727945625782,
-0.013691522181034088,
-0.0028598459903150797,
-0.0026885622646659613,
0.08180397748947144,
-0.04677446559071541,
0.018236417323350906,
0.05467779561877251,
0.032143786549568176,
-0.007254312746226788,
-0.054425615817308426,
-0.07660792022943497,
-0.0088274497538805,
-0.015612319111824036,
-0.006170264910906553,
0.034797873347997665,
-0.0295221246778965,
-0.005740437190979719,
0.024463722482323647,
0.020745504647493362,
-0.01788606494665146,
-0.022694645449519157,
-0.00650605745613575,
0.007785181049257517,
-0.04725125432014465,
-0.035910360515117645,
-0.01610668934881687,
0.03153573349118233,
0.039306189864873886,
-0.2772531509399414,
0.035373806953430176,
0.022380245849490166,
0.06892097741365433,
-0.023269155994057655,
0.011408627964556217,
0.021099399775266647,
-0.05387464165687561,
-0.04597777500748634,
0.07174694538116455,
-0.04554979130625725,
0.03589768707752228,
0.04785700514912605,
0.027851779013872147,
-0.021450849249958992,
-0.008314017206430435,
0.020552629604935646,
-0.02855451963841915,
0.006263029761612415,
-0.02018008939921856,
0.010184582322835922,
0.008943572640419006,
0.17010286450386047,
-0.005706275347620249,
-0.02823353372514248,
-0.019256435334682465,
-0.024281714111566544,
0.02542552724480629,
-0.06562945246696472,
0.027172164991497993,
-0.005239402875304222,
0.02379794605076313,
0.04416230320930481,
-0.08834323287010193,
0.03501354902982712,
0.050643935799598694,
-0.018551941961050034,
-0.0765722468495369,
0.011570128612220287,
0.013914835639297962,
0.076199971139431,
-0.01463509164750576,
-0.06411892920732498,
-0.030302761122584343,
0.03608214855194092,
-0.07153645902872086,
-0.02403051219880581,
0.007406491786241531,
0.03294824808835983,
-0.04660249501466751,
-0.008527246303856373,
-0.0489564873278141,
-0.0028009081725031137,
-0.0011434181360527873,
0.008222539909183979,
0.027203941717743874,
-0.019979313015937805,
0.003942964598536491,
-0.05745586380362511,
-0.014571648091077805,
0.07729865610599518,
0.01665516383945942,
-0.05887919291853905,
0.034893427044153214,
0.05363684520125389
] |
PMID:25089 | Influence of phosphate ligands in abolishing the conformational difference between ribonuclease A and its acid-denatured derivative. | The initial structural alteration of RNAase A due to acid denaturation (0.5 N HCl, 30 degrees C) that accompanies deamidation (without altering enzymic activity) has been dectected by spectrophotometric titration, fluorescence and ORD/CD measurements. It is shown that acid treated RNAase A has an altered conformation at neutral pH, 25 degrees C. This is characterized by the increased accessibility of buried tyrosine residue(s) towards the solvent. The most altered conformation of RNAase A is found in the 10 h acid-treated derivative. This has about 1.5 additional exposed tyrosine residues and a lesser amount of secondary structure than RNAase A. All three methods (titration, fluorescence and CD) established that the structural transition of RNAase A is biphasic. The first phase occurs within 1 h and the resulting subtle conformational change is constant up to 7 h. Following this, after the release of 0.55 mol of ammonia, the major conformational change begins. The altered conformation of the acid-denatured RNAase A could be reversed completely to the native state through a conformational change induced by substrate analogs like 2'- or 3'-CMP. Thus the monodeamidated derivative isolated from the acid-denatured RNAase A by phosphate is very similar to RNAase A in over-all conformation. The results suggest the possibility of flexibility in the RNAase A molecule that does not affect its catalytic activity, as probed through the tyrosine residues. | Influence of phosphate ligands in abolishing the conformational difference between ribonuclease A and its acid-denatured derivative. The initial structural alteration of RNAase A due to acid denaturation (0.5 N HCl, 30 degrees C) that accompanies deamidation (without altering enzymic activity) has been dectected by spectrophotometric titration, fluorescence and ORD/CD measurements. It is shown that acid treated RNAase A has an altered conformation at neutral pH, 25 degrees C. This is characterized by the increased accessibility of buried tyrosine residue(s) towards the solvent. The most altered conformation of RNAase A is found in the 10 h acid-treated derivative. This has about 1.5 additional exposed tyrosine residues and a lesser amount of secondary structure than RNAase A. All three methods (titration, fluorescence and CD) established that the structural transition of RNAase A is biphasic. The first phase occurs within 1 h and the resulting subtle conformational change is constant up to 7 h. Following this, after the release of 0.55 mol of ammonia, the major conformational change begins. The altered conformation of the acid-denatured RNAase A could be reversed completely to the native state through a conformational change induced by substrate analogs like 2'- or 3'-CMP. Thus the monodeamidated derivative isolated from the acid-denatured RNAase A by phosphate is very similar to RNAase A in over-all conformation. The results suggest the possibility of flexibility in the RNAase A molecule that does not affect its catalytic activity, as probed through the tyrosine residues. | [
-0.1060655415058136,
-0.015067723579704762,
0.0071213399060070515,
0.04665889963507652,
0.0025023275520652533,
0.015115457586944103,
0.04388951137661934,
0.023427046835422516,
-0.02834182046353817,
0.002609489718452096,
0.07060544192790985,
-0.039643142372369766,
-0.028045449405908585,
-0.01037269365042448,
-0.012202082201838493,
-0.03417182341217995,
-0.039427779614925385,
-0.028908750042319298,
-0.040295012295246124,
-0.023486781865358353,
0.012833630666136742,
0.026566091924905777,
0.051294825971126556,
-0.01471351832151413,
0.023228397592902184,
0.0417545847594738,
0.011041217483580112,
0.029147887602448463,
-0.07173498719930649,
-0.2429618537425995,
0.010221070609986782,
0.002817445434629917,
0.04365050047636032,
-0.05569475144147873,
-0.029836313799023628,
0.0732075423002243,
-0.05233317241072655,
-0.04442080855369568,
-0.03753352910280228,
0.023548919707536697,
0.050848688930273056,
0.04637300968170166,
0.03765592351555824,
-0.010417377576231956,
-0.07937707006931305,
0.012947787530720234,
-0.033526305109262466,
-0.012456769123673439,
0.06824541091918945,
0.01592640019953251,
-0.02684885822236538,
0.003937798552215099,
0.01271736714988947,
-0.040509358048439026,
0.023888610303401947,
-0.014683373272418976,
0.05791141837835312,
0.10236089676618576,
-0.0011003422550857067,
-0.028665637597441673,
0.02168603613972664,
-0.019429000094532967,
-0.13716641068458557,
0.09186159819364548,
0.10786617547273636,
0.03442405164241791,
0.04968881607055664,
-0.08226059377193451,
0.008906189352273941,
0.0670604333281517,
-0.05225767567753792,
0.01255928073078394,
-0.027111953124403954,
-0.0073766401037573814,
0.05245237424969673,
-0.03418451547622681,
0.012046432122588158,
0.008804863318800926,
0.04887254908680916,
-0.053452685475349426,
-0.04489129036664963,
0.004248734563589096,
0.009274872951209545,
0.0015338591765612364,
-0.02933879755437374,
0.01247040368616581,
0.030457237735390663,
-0.008193207904696465,
0.02537066861987114,
0.008710109628736973,
0.013142261654138565,
-0.009938402101397514,
0.05028240382671356,
-0.001871574087999761,
-0.03855757787823677,
0.11031647026538849,
0.015330016613006592,
0.015663301572203636,
0.059271104633808136,
0.3454952538013458,
-0.03970795497298241,
0.02146783284842968,
-0.08713333308696747,
-0.03855341672897339,
-0.009764937683939934,
-0.053182974457740784,
0.015553825534880161,
0.003721943823620677,
0.0448884516954422,
0.05162401124835014,
0.009727374650537968,
0.04462704807519913,
0.016729222610592842,
-0.06475623697042465,
-0.045404333621263504,
-0.008799550123512745,
-0.007431630045175552,
0.03404375538229942,
-0.04382585734128952,
-0.015779953449964523,
-0.05933494120836258,
0.020074015483260155,
0.020148007199168205,
0.026017386466264725,
0.05949138104915619,
0.03495636582374573,
-0.01356306578963995,
0.09294362366199493,
0.040432143956422806,
0.0019102649530395865,
0.03948913887143135,
-0.006823214702308178,
0.014925437048077583,
-0.025311360135674477,
0.03942890465259552,
0.002284014830365777,
-0.0052182842046022415,
0.012517675757408142,
0.03176930546760559,
0.05862964689731598,
-0.07850290089845657,
-0.04537433385848999,
-0.06559795886278152,
-0.019746895879507065,
-0.017033439129590988,
0.06876037269830704,
-0.08041580766439438,
0.04167550057172775,
-0.014139708131551743,
0.00908586848527193,
0.006131278816610575,
0.049013447016477585,
-0.028447173535823822,
0.025790713727474213,
0.00272711762227118,
-0.01664474420249462,
-0.013036753982305527,
-0.037097908556461334,
0.006989047396928072,
-0.02286655455827713,
-0.08396945148706436,
-0.025227053090929985,
-0.041987448930740356,
0.06291909515857697,
-0.0036122251767665148,
-0.026240332052111626,
0.0238631758838892,
-0.05597610026597977,
0.033442266285419464,
0.0059823342598974705,
-0.0009545335778966546,
-0.05746866390109062,
0.002841297071427107,
-0.06426037847995758,
-0.047875598073005676,
0.013136832974851131,
0.04487676918506622,
0.0021862732246518135,
0.012037504464387894,
-0.015010804869234562,
0.013061976991593838,
-0.026894863694906235,
-0.03312459588050842,
0.04187367483973503,
0.07072330266237259,
-0.021794332191348076,
-0.07203254103660583,
0.03940549120306969,
0.0430392362177372,
0.04118117317557335,
-0.0037108645774424076,
0.022892629727721214,
-0.1208595260977745,
0.02191096730530262,
-0.05878134444355965,
-0.005308700725436211,
-0.019909530878067017,
0.019826307892799377,
-0.016438055783510208,
0.02726096287369728,
0.12765827775001526,
0.04736871272325516,
-0.024347452446818352,
0.03450366109609604,
-0.028045831248164177,
0.032420556992292404,
0.019180873408913612,
0.029553599655628204,
0.006790521088987589,
0.013625363819301128,
-0.03939128294587135,
0.0012671328149735928,
-0.11404094845056534,
-0.020062264055013657,
-0.0506606288254261,
-0.0029451255686581135,
0.0020934566855430603,
0.01779910922050476,
-0.0002649022208061069,
0.02611335925757885,
0.012661099433898926,
-0.08877244591712952,
-0.03048883192241192,
-0.27942007780075073,
-0.03630046173930168,
0.0366828553378582,
-0.015534334816038609,
0.07033711671829224,
-0.07882308214902878,
-0.018455395475029945,
-0.00526435999199748,
0.017268415540456772,
-0.04160386323928833,
-0.028751466423273087,
0.03444254770874977,
0.011456450447440147,
-0.03926054388284683,
0.005695709493011236,
0.00770030589774251,
0.04121796041727066,
0.0037116925232112408,
0.06227315217256546,
-0.004135513212531805,
0.0099653834477067,
0.005040464922785759,
-0.015043554827570915,
-0.03209169581532478,
0.030871450901031494,
0.014636935666203499,
0.08929125964641571,
-0.013735273852944374,
0.0073903342708945274,
0.05385030806064606,
0.002531876554712653,
0.06358296424150467,
-0.04438851401209831,
-0.09919038414955139,
-0.028871918097138405,
0.02117045596241951,
-0.02069864049553871,
-0.05993850156664848,
-0.025946564972400665,
-0.06662142276763916,
0.05832090973854065,
0.047764625400304794,
-0.01939602755010128,
-0.04367870092391968,
-0.008582280948758125,
0.003935018554329872,
0.00442759832367301,
0.019538214430212975,
0.04787270352244377,
0.04504182189702988,
0.04112820327281952,
-0.02020825818181038,
0.0024715743493288755,
0.009469474665820599,
0.011687266640365124,
-0.01916656829416752,
0.023948775604367256,
0.017170779407024384,
-0.016103528439998627,
-0.0339771993458271,
-0.01943371817469597,
0.027498407289385796,
-0.009466033428907394,
0.0686761885881424,
-0.012483612634241581,
-0.03520061820745468,
0.02986842580139637,
-0.032867033034563065,
0.041653625667095184,
-0.046534739434719086,
0.013830138370394707,
0.10198367387056351,
-0.06429558247327805,
-0.0044856262393295765,
0.0551205612719059,
0.03503147512674332,
-0.0864953100681305,
-0.05511743202805519,
-0.07811933755874634,
-0.010122138075530529,
0.001987891737371683,
0.010624425485730171,
0.0868377760052681,
-0.04332498461008072,
-0.05191730335354805,
0.031068885698914528,
0.036510735750198364,
0.02524670958518982,
-0.00018944471958093345,
0.02993142232298851,
0.07481911033391953,
-0.033306561410427094,
-0.012696349993348122,
-0.030588971450924873,
0.0521361380815506,
0.036212749779224396,
-0.25297847390174866,
-0.03372568264603615,
0.03129942715167999,
0.0180805716663599,
-0.04093901067972183,
0.03187279775738716,
0.042729031294584274,
-0.039526380598545074,
-0.0416327640414238,
0.016625389456748962,
0.01776066981256008,
0.040146324783563614,
0.01592273637652397,
0.043121062219142914,
0.02399325557053089,
0.000671371235512197,
0.06891363859176636,
-0.07457644492387772,
0.034933339804410934,
-0.031056270003318787,
-0.01651811972260475,
-0.033690355718135834,
0.1449529081583023,
-0.0542147196829319,
0.017733031883835793,
0.07186589390039444,
-0.03909226879477501,
0.034570612013339996,
-0.027823280543088913,
-0.011539504863321781,
-0.04390503838658333,
0.029956480488181114,
0.055021144449710846,
-0.03984490782022476,
0.05235476419329643,
0.04023723676800728,
-0.028847619891166687,
0.002042084466665983,
-0.024598756805062294,
0.015008464455604553,
0.027713127434253693,
-0.0028538107872009277,
-0.07231713086366653,
-0.029849322512745857,
0.09520170837640762,
-0.061400096863508224,
-0.05021538957953453,
0.0020375647582113743,
-0.009030704386532307,
-0.012284612283110619,
0.025617258623242378,
-0.017213372513651848,
0.012730220332741737,
0.036348678171634674,
-0.029187215492129326,
0.006640789099037647,
-0.06138812378048897,
0.012379445135593414,
0.025613101199269295,
-0.018184958025813103,
0.025004249066114426,
0.0011898342054337263,
-0.02010256052017212,
0.06973621249198914,
0.02655523642897606
] |
PMID:25090 | Physicochemical properties of cartilage proteoglycans extracted by lanthanum chloride. | Bovine nasal cartilage was extracted with 0.5 M LaCl3 and the extract then diluted with nine volumes of water. The resulting precipitated (PLaCl3) contained the proteoglycan subunits, together with minor protein components, but was essentially free from hyaluronic acid. The properties of PLaCl3 were investigated by chemical analysis, electrophoresis, viscometry and analytical ultracentrifugation, and the results compared with those for proteoglycan obtained by caesium chloride density gradient centrifugation of 2 M CaCl2 cartilage extracts. Proteoglycan subunits (A1D1) prepared from PLaCl3 showed identical properties to those obtained from other high ionic strength cartilage extracts. | Physicochemical properties of cartilage proteoglycans extracted by lanthanum chloride. Bovine nasal cartilage was extracted with 0.5 M LaCl3 and the extract then diluted with nine volumes of water. The resulting precipitated (PLaCl3) contained the proteoglycan subunits, together with minor protein components, but was essentially free from hyaluronic acid. The properties of PLaCl3 were investigated by chemical analysis, electrophoresis, viscometry and analytical ultracentrifugation, and the results compared with those for proteoglycan obtained by caesium chloride density gradient centrifugation of 2 M CaCl2 cartilage extracts. Proteoglycan subunits (A1D1) prepared from PLaCl3 showed identical properties to those obtained from other high ionic strength cartilage extracts. | [
-0.07177271693944931,
-0.04710710793733597,
0.04348606616258621,
0.01937197893857956,
-0.006006784737110138,
0.00457961019128561,
0.0368257537484169,
0.01265512965619564,
-0.04222023859620094,
-0.043155353516340256,
-0.0018072717357426882,
-0.09486240893602371,
-0.035329125821590424,
-0.02251547947525978,
-0.018423933535814285,
0.005604073870927095,
0.018691999837756157,
0.03555607795715332,
-0.09473923593759537,
-0.015890751034021378,
0.0007694878149777651,
-0.05241989344358444,
-0.018299246206879616,
-0.03014642745256424,
0.019390137866139412,
0.04723571613430977,
-0.026841850951313972,
0.024707727134227753,
-0.01690610870718956,
-0.20820674300193787,
0.0006658075144514441,
-0.009021210484206676,
-0.012698378413915634,
-0.019895171746611595,
0.030639784410595894,
0.005731396842747927,
-0.048524320125579834,
0.03447659686207771,
-0.017875028774142265,
0.031702954322099686,
0.00801183097064495,
0.019833695143461227,
-0.02811761014163494,
0.050630949437618256,
0.08004339039325714,
-0.0012565539218485355,
0.004354740958660841,
0.01337266806513071,
0.09288386255502701,
0.0639757364988327,
0.043303314596414566,
0.030213886871933937,
0.01559063047170639,
0.03992790728807449,
-0.027728265151381493,
0.014009534381330013,
0.017497658729553223,
0.05470442399382591,
0.0713968276977539,
0.03507290780544281,
0.013785848394036293,
0.058947741985321045,
-0.09601123631000519,
0.08078986406326294,
0.0255763940513134,
0.0480196550488472,
0.003466241527348757,
-0.02659001015126705,
0.06021477282047272,
0.07612670958042145,
-0.04379407688975334,
-0.010303311981260777,
0.04880034551024437,
-0.0003587951650843024,
0.022897707298398018,
-0.020581301301717758,
-0.014342065900564194,
0.024793701246380806,
0.028692619875073433,
0.06607641279697418,
0.02004431001842022,
0.07925257086753845,
0.04482538253068924,
-0.017320893704891205,
-0.04777982831001282,
0.026629183441400528,
-0.015314478427171707,
-0.005084159784018993,
-0.00578311225399375,
0.01792815327644348,
-0.008314855396747589,
0.013967614620923996,
-0.05734078213572502,
0.04917481169104576,
-0.07874263823032379,
0.019932324066758156,
0.017462635412812233,
-0.00962846353650093,
0.010214396752417088,
0.388251930475235,
-0.03162490949034691,
0.03383995592594147,
0.008226255886256695,
-0.06404266506433487,
-0.007420085836201906,
-0.010130463168025017,
0.018710218369960785,
0.03824692592024803,
0.013223477639257908,
0.0560116171836853,
0.07975532114505768,
0.04135816916823387,
-0.041649799793958664,
-0.02243652753531933,
0.010548163205385208,
-0.016902601346373558,
0.053403593599796295,
0.017433613538742065,
0.0049473377875983715,
0.008478809148073196,
-0.07067102938890457,
-0.02190392091870308,
0.08569258451461792,
-0.03727388009428978,
0.033620744943618774,
0.03962104767560959,
0.04006831720471382,
0.08197394013404846,
0.044182464480400085,
0.04910075664520264,
0.0012278015492483974,
0.04358481615781784,
-0.014112257398664951,
-0.036750391125679016,
-0.0007786131463944912,
-0.043150950223207474,
0.008827720768749714,
-0.025523263961076736,
-0.01903749443590641,
0.04332506284117699,
-0.04400179907679558,
-0.047450244426727295,
0.00808667577803135,
-0.02633092924952507,
-0.03458934277296066,
0.04960450902581215,
-0.027722492814064026,
0.03971296176314354,
-0.09324119985103607,
0.07455466687679291,
-0.00007968011050252244,
0.041360966861248016,
-0.03568489849567413,
0.04822617024183273,
0.051594704389572144,
-0.032364290207624435,
0.0005011425237171352,
-0.03064267709851265,
0.0009902685414999723,
-0.0006651976727880538,
-0.027239210903644562,
-0.05414426326751709,
0.054438602179288864,
0.10270336270332336,
0.00710464920848608,
-0.036590252071619034,
-0.0030713703017681837,
0.02246507629752159,
0.03960409760475159,
-0.026557931676506996,
0.03237934783101082,
-0.0006745564751327038,
-0.03711654618382454,
-0.01895076595246792,
-0.024513904005289078,
0.07611139118671417,
-0.04682247340679169,
-0.010822736658155918,
0.0373549610376358,
0.009165960364043713,
0.005927159916609526,
0.022203614935278893,
-0.022346898913383484,
0.0208298172801733,
0.024569189175963402,
-0.040853291749954224,
-0.03978895768523216,
0.07066010683774948,
0.0035585558507591486,
0.04898897185921669,
-0.0036332416348159313,
0.0198435727506876,
-0.11164344847202301,
-0.003693027189001441,
-0.06311320513486862,
-0.020550444722175598,
-0.0025456827133893967,
-0.01448154915124178,
0.013398281298577785,
-0.05190405994653702,
0.08514143526554108,
0.009882553480565548,
-0.03148314729332924,
0.03165303170681,
0.04845836013555527,
0.055397484451532364,
0.020574131980538368,
0.027592504397034645,
0.0541965588927269,
-0.06600875407457352,
-0.04776596277952194,
0.024600790813565254,
-0.07190922647714615,
-0.044737908989191055,
-0.05836426839232445,
0.0325973816215992,
0.007389436475932598,
-0.009665695019066334,
0.017540477216243744,
-0.024969352409243584,
0.04315599054098129,
-0.05269872024655342,
-0.08327678591012955,
-0.29732534289360046,
0.07088872045278549,
0.02001393586397171,
0.015437282621860504,
0.08992481976747513,
-0.06311684846878052,
0.05921299383044243,
0.005768293514847755,
0.020633751526474953,
-0.05583985149860382,
0.05511161684989929,
0.07800587266683578,
-0.057347457855939865,
0.015288803726434708,
0.0029972780030220747,
0.04917105659842491,
0.05044444277882576,
-0.038119327276945114,
0.01527108158916235,
0.0024199143517762423,
0.0030581681057810783,
0.030846592038869858,
-0.02794591709971428,
0.05262468755245209,
0.056535281240940094,
-0.02967372164130211,
0.09074240922927856,
-0.062426354736089706,
-0.024734361097216606,
-0.021927593275904655,
0.00034992428845725954,
0.023695705458521843,
-0.030436448752880096,
-0.0990854799747467,
-0.0034512884449213743,
0.006308674346655607,
-0.013277562335133553,
0.013174888677895069,
0.0032832163851708174,
-0.02296382561326027,
0.0010173259070143104,
0.037087101489305496,
-0.030045393854379654,
-0.05413328856229782,
-0.04041382670402527,
-0.026457546278834343,
-0.008294804953038692,
0.018789591267704964,
-0.01524177473038435,
-0.008485332131385803,
0.012293187901377678,
0.03801015019416809,
-0.029239818453788757,
-0.04746098816394806,
-0.02552942745387554,
0.00935597438365221,
0.013004115782678127,
0.009942276403307915,
-0.0788981020450592,
-0.04534700885415077,
-0.010489151813089848,
-0.02872657962143421,
0.0035219343844801188,
0.017842503264546394,
-0.04467322304844856,
-0.024334203451871872,
0.006099902093410492,
-0.03165630251169205,
0.012331390753388405,
-0.025450071319937706,
-0.023797906935214996,
0.0617695190012455,
-0.04852960631251335,
-0.017276378348469734,
0.020354967564344406,
0.04046835005283356,
-0.009616299532353878,
0.005697045475244522,
-0.06492285430431366,
0.051936883479356766,
-0.021844368427991867,
0.012350651435554028,
0.018059514462947845,
0.044219862669706345,
0.005909261293709278,
-0.011624984443187714,
-0.0013641805853694677,
-0.014795392751693726,
-0.041451115161180496,
-0.013007322326302528,
0.041963592171669006,
-0.02830708585679531,
-0.04140206053853035,
-0.02406882308423519,
0.0719720870256424,
-0.002852240577340126,
-0.2614215612411499,
-0.045672785490751266,
0.07026488333940506,
0.00460511539131403,
-0.0125782061368227,
-0.004906048998236656,
0.02273997850716114,
-0.053751908242702484,
0.01227020937949419,
0.06477876752614975,
0.06984154880046844,
0.04773036763072014,
-0.004408272448927164,
-0.05223781615495682,
0.006265818141400814,
-0.023337265476584435,
0.05595742166042328,
-0.05462639778852463,
0.04833759739995003,
-0.01841679960489273,
-0.013931486755609512,
-0.07497486472129822,
0.15660721063613892,
0.025435127317905426,
-0.04804520308971405,
0.016497980803251266,
0.023595552891492844,
-0.023571457713842392,
-0.06649799644947052,
-0.043095771223306656,
-0.007980633527040482,
-0.028432900086045265,
0.029156744480133057,
-0.03565624728798866,
-0.02309226617217064,
0.06124425306916237,
-0.000780379050411284,
-0.044459011405706406,
-0.022190431132912636,
0.003991558216512203,
0.06889782100915909,
-0.02260465919971466,
-0.05629020556807518,
-0.03230100870132446,
0.026908237487077713,
-0.08796712011098862,
0.018046332523226738,
-0.054732177406549454,
-0.02060898020863533,
0.010609757155179977,
-0.03138938918709755,
-0.0756211057305336,
-0.023783065378665924,
-0.03187363222241402,
-0.03630874678492546,
0.01650371216237545,
0.041503872722387314,
-0.01634855940937996,
-0.03852204605937004,
0.03155570477247238,
0.025445017963647842,
-0.06964117288589478,
-0.0345422700047493,
0.08503937721252441,
0.027696391567587852
] |
PMID:25091 | The involvement of oxygen radicals during the autoxidation of adrenalin. | 1. In unbuffered alkaline solutions, autoxidizing adrenalin generates superoxide anions: both the scavenging by adrenalin itself, leading to adrenochrome, and the formation of nitrite from hydroxylamine are inhibited by superoxide dismutase. No hydroxyl radical could be detected. 2. The yield of hydrogen peroxide increases with pH in a way similar to that of adrenochrome and nitrite. The dissociated form of adrenalin (pK = 8.5) is proposed as the source of superoxide anions. 3. Superoxide dismutase delays rather than inhibits the reaction. In addition to the diminished formation of adrenochrome due to the scavenging of superoxide anions and re-reduction of the semiquinone by hydrogen peroxide, respectively, adrenochrome is further removed by hydrogen peroxide, with final products absorbing at 310 nm. 4. The diminished inhibitory effect of superoxide dismutase above pH 10 is due to superoxide-independent reactions. This effect is masked by the alkaline conversion of adrenochrome to indole compounds. 5. It is concluded that monitoring the absorption of adrenochrome in alkaline solutions does not produce reliable evidence for superoxide anions. | The involvement of oxygen radicals during the autoxidation of adrenalin. 1. In unbuffered alkaline solutions, autoxidizing adrenalin generates superoxide anions: both the scavenging by adrenalin itself, leading to adrenochrome, and the formation of nitrite from hydroxylamine are inhibited by superoxide dismutase. No hydroxyl radical could be detected. 2. The yield of hydrogen peroxide increases with pH in a way similar to that of adrenochrome and nitrite. The dissociated form of adrenalin (pK = 8.5) is proposed as the source of superoxide anions. 3. Superoxide dismutase delays rather than inhibits the reaction. In addition to the diminished formation of adrenochrome due to the scavenging of superoxide anions and re-reduction of the semiquinone by hydrogen peroxide, respectively, adrenochrome is further removed by hydrogen peroxide, with final products absorbing at 310 nm. 4. The diminished inhibitory effect of superoxide dismutase above pH 10 is due to superoxide-independent reactions. This effect is masked by the alkaline conversion of adrenochrome to indole compounds. 5. It is concluded that monitoring the absorption of adrenochrome in alkaline solutions does not produce reliable evidence for superoxide anions. | [
-0.06240396574139595,
0.03207919001579285,
-0.01746770553290844,
0.020112300291657448,
0.03468483313918114,
-0.04046964272856712,
0.06840294599533081,
0.007283305749297142,
-0.08579071611166,
-0.05842384696006775,
0.02398601733148098,
-0.006375969387590885,
0.05285272002220154,
0.08496885746717453,
-0.05041325464844704,
-0.021487461403012276,
0.024092471227049828,
0.004941146820783615,
-0.1364687830209732,
0.03640769049525261,
-0.035746071487665176,
0.01472844835370779,
0.03980936110019684,
0.021605458110570908,
0.08784157782793045,
0.04298200085759163,
0.00857947301119566,
0.011784075759351254,
-0.05203906074166298,
-0.17908188700675964,
0.010639894753694534,
-0.02079099230468273,
0.009380782954394817,
-0.0019404413178563118,
-0.006929140072315931,
0.00443735858425498,
-0.04597179964184761,
0.04404006525874138,
0.013929818756878376,
0.05668431147933006,
0.044746171683073044,
0.051942113786935806,
0.03018367290496826,
0.04286878928542137,
0.010504739359021187,
0.032221719622612,
0.03752804547548294,
0.02220509760081768,
0.1075609028339386,
-0.029741987586021423,
0.02949753776192665,
0.048357464373111725,
-0.026962697505950928,
0.061031606048345566,
0.0061746262945234776,
-0.058394208550453186,
-0.011670785024762154,
0.05083826184272766,
-0.05449153482913971,
0.03825061023235321,
-0.005963310599327087,
0.08134877681732178,
-0.13873831927776337,
0.03319256007671356,
0.059168703854084015,
0.019214674830436707,
0.02328413538634777,
0.02771717682480812,
0.01981615647673607,
-0.014748252928256989,
-0.030075956135988235,
0.0388343445956707,
0.0006829509511590004,
0.003091601189225912,
0.034521881490945816,
-0.029675735160708427,
-0.007045078556984663,
-0.03521624207496643,
-0.01620328426361084,
0.0007541076047345996,
0.03979773819446564,
0.008556593209505081,
0.003182436805218458,
-0.004485876765102148,
0.029187610372900963,
-0.033253174275159836,
0.015100430697202682,
0.03036273829638958,
0.013633430004119873,
-0.04990740120410919,
-0.021494217216968536,
-0.059671007096767426,
0.023709934204816818,
0.02862529456615448,
-0.06572213023900986,
0.021414106711745262,
-0.03766407072544098,
-0.04024646803736687,
-0.036245740950107574,
0.3478814363479614,
-0.05106087028980255,
-0.0012011518701910973,
-0.06860540807247162,
-0.03913189470767975,
-0.016956880688667297,
0.041600778698921204,
-0.053621236234903336,
-0.030990470200777054,
-0.007448923774063587,
0.02255821041762829,
0.0050086467526853085,
0.006310846656560898,
0.017282992601394653,
-0.08839540928602219,
-0.021415552124381065,
0.028574930503964424,
0.021260306239128113,
-0.018140869215130806,
-0.011572509072721004,
0.018211279064416885,
0.005225182976573706,
-0.020246924832463264,
0.04277036711573601,
0.03397919982671738,
-0.009936879388988018,
0.024709325283765793,
0.03238533064723015,
0.07383622974157333,
0.03923546522855759,
0.02753298170864582,
0.014689178206026554,
0.015830615535378456,
-0.02941594459116459,
0.0032564904540777206,
-0.0629332959651947,
-0.03746502846479416,
0.0018584001809358597,
-0.0719928964972496,
0.015140218660235405,
-0.03957468643784523,
-0.05862874910235405,
-0.007790020201355219,
-0.03255059942603111,
-0.08800716698169708,
-0.05657665804028511,
0.07052674889564514,
0.07490867376327515,
-0.009158588945865631,
-0.033820439130067825,
-0.02498258277773857,
-0.015784887596964836,
0.06318160891532898,
-0.024978559464216232,
0.10298103839159012,
-0.016921881586313248,
0.04510295018553734,
-0.025692643597722054,
-0.02587355673313141,
-0.013359719887375832,
-0.013514885678887367,
-0.08374643325805664,
-0.029184535145759583,
-0.013196083717048168,
0.11188705265522003,
0.04823878034949303,
0.0019010694231837988,
-0.008013727143406868,
0.04759079962968826,
-0.007265135180205107,
0.010259129106998444,
0.031099511310458183,
-0.023428577929735184,
-0.018436098471283913,
-0.06418312340974808,
0.013064512051641941,
0.017087848857045174,
0.04293369501829147,
0.007321114186197519,
0.03516359627246857,
-0.023416584357619286,
0.10139540582895279,
0.03548991307616234,
0.017571579664945602,
0.04576965048909187,
0.00800122320652008,
-0.08596273511648178,
-0.0676501914858818,
0.017086343839764595,
-0.010832300409674644,
0.024713434278964996,
-0.05570250377058983,
-0.0035315637942403555,
-0.102229543030262,
-0.015357580035924911,
-0.03026343323290348,
-0.02005084417760372,
-0.06153164431452751,
-0.05514336749911308,
-0.0046975817531347275,
-0.009505866095423698,
0.09688743203878403,
-0.044589705765247345,
-0.001322215422987938,
0.022213928401470184,
0.019923578947782516,
0.023376891389489174,
0.026869390159845352,
-0.0006865134346298873,
0.007982087321579456,
-0.002778775990009308,
-0.005099169444292784,
0.014592600055038929,
-0.029696771875023842,
0.024210261180996895,
-0.06901329010725021,
0.05192195251584053,
-0.03367237746715546,
-0.021865980699658394,
0.09092328697443008,
0.01805998384952545,
0.08132857084274292,
-0.03297993913292885,
-0.034254636615514755,
-0.2793784439563751,
0.005357290618121624,
-0.000051639210141729563,
-0.01301115658134222,
0.06228816509246826,
-0.08429136872291565,
0.03196714445948601,
-0.06671301275491714,
0.004648389760404825,
-0.06349906325340271,
0.01671791635453701,
0.02168879471719265,
0.01411147229373455,
-0.04024377837777138,
0.0837707370519638,
0.024016454815864563,
0.025430843234062195,
-0.04591868445277214,
0.06756497174501419,
0.049445390701293945,
-0.006215087603777647,
-0.040063660591840744,
0.04245571419596672,
0.010472523048520088,
0.03686332330107689,
-0.00045487386523745954,
0.12689460813999176,
0.005350352264940739,
-0.027880774810910225,
-0.03312712535262108,
-0.015612112358212471,
0.03232930228114128,
-0.05961956828832626,
-0.08786103129386902,
-0.02233179472386837,
-0.051465440541505814,
0.04374757036566734,
-0.036224447190761566,
-0.03437209129333496,
-0.04009467363357544,
0.00034989402047358453,
0.04843690246343613,
-0.017174022272229195,
-0.03155870735645294,
-0.022093385457992554,
-0.07282155007123947,
0.008428826928138733,
0.009149608202278614,
0.001094950013794005,
-0.017995746806263924,
0.02229289337992668,
0.03136804699897766,
0.03254246339201927,
-0.022976970300078392,
0.035605352371931076,
-0.015649590641260147,
-0.05521930381655693,
0.0343749038875103,
-0.0318969264626503,
-0.06946771591901779,
0.011185245588421822,
-0.04148576036095619,
0.012868738733232021,
0.009010565467178822,
0.001813005656003952,
0.06868705153465271,
-0.00811225175857544,
-0.012611514888703823,
0.04764271527528763,
0.02964337356388569,
0.05088818818330765,
0.14049482345581055,
0.015615532174706459,
-0.008332720957696438,
0.03358982130885124,
0.05181146040558815,
-0.05358630046248436,
-0.03202167525887489,
-0.04807663708925247,
0.06530884653329849,
-0.012583580799400806,
-0.06717788428068161,
0.06369350105524063,
0.018503792583942413,
-0.001372603583149612,
0.02632633037865162,
-0.011684819124639034,
-0.0045989383943378925,
0.030527085065841675,
-0.006096667610108852,
-0.04846411943435669,
0.029908563941717148,
-0.028530871495604515,
-0.024362221360206604,
0.06483761221170425,
0.03951648995280266,
-0.24262751638889313,
-0.04026803746819496,
0.04746340587735176,
-0.023220419883728027,
-0.037651363760232925,
0.013093576766550541,
-0.01691628061234951,
-0.04309610277414322,
-0.013720105402171612,
0.0360649898648262,
-0.006295942235738039,
0.011156049557030201,
0.0671757310628891,
-0.003176662838086486,
-0.024093251675367355,
0.04284251108765602,
0.02227509580552578,
0.03383675590157509,
0.023697948083281517,
-0.025291647762060165,
-0.015129503794014454,
-0.000002401710617050412,
0.13059446215629578,
-0.07051196694374084,
-0.03223811835050583,
0.07454081624746323,
-0.025517107918858528,
0.03774949163198471,
-0.029155056923627853,
0.0089480672031641,
0.0045315721072256565,
0.05798364803195,
-0.014583469368517399,
-0.013945111073553562,
0.01388750970363617,
0.036802977323532104,
-0.0638260543346405,
0.008065986447036266,
0.030101319774985313,
-0.04868559539318085,
-0.020263763144612312,
-0.03424222394824028,
-0.08721274882555008,
-0.05254999175667763,
0.06454732269048691,
-0.06576766818761826,
-0.05163995549082756,
-0.009155357256531715,
0.03597337007522583,
-0.028029397130012512,
-0.02574704773724079,
-0.03278452157974243,
0.07993046939373016,
0.059633705765008926,
0.013487848453223705,
0.013351996429264545,
-0.0849182978272438,
-0.028430208563804626,
0.04723813384771347,
0.021104950457811356,
-0.027767634019255638,
0.03914589807391167,
0.0011521285632625222,
0.010097573511302471,
0.07924238592386246
] |
PMID:25092 | A protease-like permeability factor in the guinea pig skin. 1. Partial purification and characterization. | A permeability factor was extracted in a latent form from guinea pig skin and separated by ammonium sulfate fractionation into the pseudoglobulin fraction (30--50% saturation). The activation of the latent form of the permeability factor seemed to be caused in the desalting step by gel filtration with Sephadex G-50. The factor was partially purified by streptomycin treatment and column chromatography using hydroxyapatite, diethylaminoethyl cellulose and Sephadex G-75, in this order. Gel filtration showed that its molecular weight was approx. 35000. Its permeability activity was heat stable at 61 degrees C for 60 min at neutral pH, resistant at pH 5--10 and at ionic strengths from deionized water to 1 M NaCl at 4 degrees C. Its activity was transient and suppressed by guinea pig serum, but insensitive to an anti-histamic agent (triprolidine). Furthermore, its permeability activity was inhibited by diisopropylfluorophosphate, soybean trypsin inhibitor and leupeptin, and completely adsorbed by soybean trypsin inhibitor affinity column. These findings suggested that the permeability factor was a serine-type protease. | A protease-like permeability factor in the guinea pig skin. 1. Partial purification and characterization. A permeability factor was extracted in a latent form from guinea pig skin and separated by ammonium sulfate fractionation into the pseudoglobulin fraction (30--50% saturation). The activation of the latent form of the permeability factor seemed to be caused in the desalting step by gel filtration with Sephadex G-50. The factor was partially purified by streptomycin treatment and column chromatography using hydroxyapatite, diethylaminoethyl cellulose and Sephadex G-75, in this order. Gel filtration showed that its molecular weight was approx. 35000. Its permeability activity was heat stable at 61 degrees C for 60 min at neutral pH, resistant at pH 5--10 and at ionic strengths from deionized water to 1 M NaCl at 4 degrees C. Its activity was transient and suppressed by guinea pig serum, but insensitive to an anti-histamic agent (triprolidine). Furthermore, its permeability activity was inhibited by diisopropylfluorophosphate, soybean trypsin inhibitor and leupeptin, and completely adsorbed by soybean trypsin inhibitor affinity column. These findings suggested that the permeability factor was a serine-type protease. | [
-0.07386692613363266,
-0.02702224813401699,
-0.03654424846172333,
-0.002328864298760891,
0.04432082921266556,
-0.03761063888669014,
0.08747782558202744,
0.012155003845691681,
-0.08260157704353333,
-0.0020767315290868282,
-0.031107155606150627,
-0.12397650629281998,
-0.01246702391654253,
0.05386855825781822,
-0.004751282744109631,
0.021740533411502838,
0.028870314359664917,
0.01511495653539896,
-0.05378022417426109,
-0.0019854025449603796,
0.0038963945116847754,
0.0008002226823009551,
0.016961809247732162,
-0.053519006818532944,
0.019367538392543793,
0.019786939024925232,
0.015130594372749329,
0.018237553536891937,
-0.011305182240903378,
-0.23166406154632568,
-0.019235776737332344,
-0.013369442895054817,
-0.003978378139436245,
-0.04301279038190842,
0.016379984095692635,
0.07470027357339859,
-0.0062680309638381,
-0.04144493490457535,
-0.002789510879665613,
0.06064824387431145,
0.08080098032951355,
0.03587624058127403,
0.0015982369659468532,
0.03371632471680641,
0.05600747466087341,
0.03686818480491638,
-0.021598760038614273,
0.028292696923017502,
0.05688924342393875,
0.0015055924886837602,
0.05969997122883797,
-0.006056568585336208,
0.003491644747555256,
0.06036422774195671,
0.008927835151553154,
0.022833092138171196,
-0.0012082438915967941,
0.028535539284348488,
-0.07361120730638504,
-0.00843561626970768,
0.012911481782793999,
-0.0017551296623423696,
-0.06950347870588303,
0.08780553191900253,
0.06086280941963196,
-0.007104432210326195,
0.00425328966230154,
-0.07384727150201797,
0.02140989899635315,
0.058072641491889954,
-0.04015638306736946,
0.08164579421281815,
-0.06497278809547424,
-0.020474396646022797,
-0.004108017776161432,
0.029004208743572235,
0.010705903172492981,
-0.002181209623813629,
0.06428834795951843,
0.06536534428596497,
-0.04118211194872856,
0.038320738822221756,
-0.0035662867594510317,
-0.03335491940379143,
-0.03578636422753334,
-0.021590393036603928,
0.0238190870732069,
-0.03392134979367256,
-0.0005206366186030209,
-0.0006030761869624257,
-0.04206480458378792,
-0.02178012952208519,
0.046173665672540665,
-0.003405938157811761,
-0.029658377170562744,
-0.004703070502728224,
0.07044778019189835,
-0.021026985719799995,
0.006733458023518324,
0.37382131814956665,
-0.022642306983470917,
-0.020457888022065163,
-0.08803302049636841,
0.01396796852350235,
0.006942771840840578,
-0.024192865937948227,
0.011356222443282604,
0.04236748814582825,
0.012567245401442051,
0.08910894393920898,
0.07106845825910568,
0.03758125379681587,
0.04206034168601036,
-0.059282463043928146,
0.029378356412053108,
0.018912065774202347,
-0.03641331195831299,
-0.01447084080427885,
-0.013625528663396835,
0.02218032255768776,
0.008478410542011261,
-0.008514131419360638,
0.0435730405151844,
-0.02032722719013691,
0.015639126300811768,
0.0020168309565633535,
-0.04284185916185379,
0.09594656527042389,
0.05274543911218643,
-0.005330988205969334,
0.018281031399965286,
0.024439549073576927,
-0.0656551867723465,
0.041404951363801956,
0.020935479551553726,
-0.03719377890229225,
-0.027289632707834244,
-0.032451387494802475,
-0.04427085071802139,
0.009934715926647186,
-0.021793950349092484,
-0.01238503772765398,
-0.04793465510010719,
-0.037604231387376785,
-0.04015854001045227,
0.05869457125663757,
0.00873982161283493,
0.03326528146862984,
-0.012433962896466255,
-0.005135663319379091,
0.004716068040579557,
0.06867780536413193,
-0.020276691764593124,
0.040475569665431976,
0.004164937883615494,
-0.009037429466843605,
-0.009436351247131824,
-0.04744819179177284,
0.04836966469883919,
-0.018730200827121735,
-0.01716579496860504,
-0.042863719165325165,
-0.03765947371721268,
0.03687398508191109,
0.039461154490709305,
-0.007625360041856766,
-0.02286357991397381,
0.04290786013007164,
0.0015681792283430696,
-0.0036709457635879517,
0.016360118985176086,
0.059235863387584686,
-0.1046440377831459,
-0.004405226558446884,
-0.08082237094640732,
0.0346483439207077,
-0.02414928562939167,
0.013924085535109043,
0.06453467905521393,
-0.00944563839584589,
0.03574284538626671,
-0.021119404584169388,
-0.053410742431879044,
-0.029487308114767075,
0.00906334724277258,
-0.00008419220830546692,
-0.0260179303586483,
0.025643374770879745,
0.05140668526291847,
0.04991792142391205,
-0.01838678866624832,
-0.013131481595337391,
-0.0815613716840744,
0.05740975961089134,
-0.03045354038476944,
-0.03322961553931236,
-0.035460829734802246,
-0.023320740088820457,
-0.037450049072504044,
-0.04794399440288544,
0.0859115794301033,
0.01805209182202816,
0.020265614613890648,
-0.0003694793558679521,
0.024272089824080467,
0.043338946998119354,
0.045746490359306335,
-0.03002220392227173,
0.028227047994732857,
-0.033620864152908325,
-0.05414213612675667,
0.012715739198029041,
-0.028621044009923935,
-0.0164381992071867,
-0.025181541219353676,
0.07152211666107178,
0.010065865702927113,
-0.004780804738402367,
0.08065711706876755,
0.02179318480193615,
0.013999342918395996,
-0.039348624646663666,
-0.11030635237693787,
-0.2951159179210663,
0.02579730749130249,
0.012266158126294613,
-0.01205756701529026,
0.09262115508317947,
-0.04296942800283432,
-0.02340623363852501,
-0.05498826131224632,
-0.005221758969128132,
-0.024362457916140556,
0.10606979578733444,
0.0757591649889946,
-0.047918882220983505,
0.025274895131587982,
0.01937551237642765,
0.05573127418756485,
0.03744352236390114,
-0.0314069502055645,
0.024521246552467346,
-0.016354499384760857,
-0.017992103472352028,
-0.014005056582391262,
0.04449998214840889,
0.053506039083004,
0.01762937381863594,
0.035401586443185806,
0.10827036947011948,
-0.013508149422705173,
-0.05870191380381584,
-0.0405467227101326,
0.05751928687095642,
0.05692783743143082,
-0.03979349508881569,
-0.10823353379964828,
-0.03757050260901451,
-0.03005635179579258,
-0.006089127156883478,
-0.014021742157638073,
0.011394907720386982,
-0.005729569587856531,
0.005493729840964079,
0.05058297514915466,
0.012828979641199112,
-0.037070512771606445,
-0.04550466686487198,
0.000188006364624016,
0.045957282185554504,
0.04742906987667084,
-0.05960499867796898,
-0.006555515341460705,
0.08396942168474197,
-0.06022539734840393,
0.03492952883243561,
-0.03450595960021019,
0.0076607596129179,
-0.03060910850763321,
-0.004112218506634235,
0.014494962058961391,
-0.045507293194532394,
0.00951179675757885,
0.018222171813249588,
-0.02194996550679207,
0.008030412718653679,
-0.020640352740883827,
-0.07915526628494263,
-0.0040408833883702755,
-0.003904345678165555,
-0.027811944484710693,
0.02486236020922661,
-0.014776227064430714,
-0.028605422005057335,
0.09922590851783752,
-0.02705024741590023,
-0.0007969337166287005,
0.06763249635696411,
0.014469441957771778,
0.057930029928684235,
-0.02935183420777321,
-0.045958440750837326,
-0.01228365395218134,
0.02773897536098957,
-0.049111850559711456,
-0.005010060966014862,
-0.025228627026081085,
0.06698407977819443,
0.015875069424510002,
-0.007996983826160431,
-0.04312611743807793,
0.013636307790875435,
0.0495980829000473,
0.021456142887473106,
-0.03604366257786751,
-0.03221376612782478,
-0.02954445220530033,
0.036521028727293015,
-0.05660179629921913,
-0.2650669813156128,
0.019689364358782768,
0.0781516581773758,
-0.009473915211856365,
-0.03384215384721756,
0.003957789391279221,
0.08221849054098129,
-0.020129621028900146,
-0.02854888141155243,
-0.004081045743077993,
0.0037160837091505527,
-0.0011442932300269604,
0.05677054077386856,
-0.0029372884891927242,
-0.033026859164237976,
-0.005702899768948555,
0.029310964047908783,
0.006265319883823395,
0.022605694830417633,
-0.03739988058805466,
-0.01385441329330206,
-0.08988567441701889,
0.14756318926811218,
-0.012791749089956284,
0.0042853280901908875,
0.029065540060400963,
0.010746264830231667,
0.002414156449958682,
0.011602479964494705,
0.01204740721732378,
0.02887909859418869,
-0.022913562133908272,
0.06615930050611496,
-0.008848682977259159,
-0.0038425184320658445,
-0.05154374986886978,
0.015149805694818497,
0.002904002321884036,
-0.0038848964031785727,
-0.03202031925320625,
0.01726379431784153,
-0.012664020992815495,
-0.054067496210336685,
-0.03005536086857319,
0.029704533517360687,
-0.13553152978420258,
0.003289794782176614,
-0.040155429393053055,
0.021424930542707443,
-0.005584989208728075,
0.015286891721189022,
-0.051056694239377975,
0.021691862493753433,
-0.031831540167331696,
0.05791638419032097,
-0.06181774660944939,
0.042934272438287735,
0.015744628384709358,
-0.030406229197978973,
0.03055688738822937,
-0.01978500559926033,
0.011159595102071762,
-0.030684664845466614,
0.052848540246486664,
0.03611551970243454
] |
PMID:25094 | The mobility of methylmercury in biological systems. | Toxicology studies indicate that methylmercury in humans and other species is bonded to sulfhydryl ligands and that the methylmercury in such complexes is labile even though their thermodynamic stability is large. It is shown in this paper that bimolecular nucleophilic displacement of complexed ligand by sulfhydryl-deprotonated ligand is the major pathway for ligand exchange at physiological pH, while at the pH of the stomach the proton-assisted dissociation of the complex is the predominant means by which exchange occurs. The dynamic and equilibrium aspects of the distribution of methylmercury between chloride and sulfhydryl ligands under the solution conditions of the stomach are also considered with respect to a possible role for lipid-soluble CH3HgCl in the absorption of methylmercury from the stomach. | The mobility of methylmercury in biological systems. Toxicology studies indicate that methylmercury in humans and other species is bonded to sulfhydryl ligands and that the methylmercury in such complexes is labile even though their thermodynamic stability is large. It is shown in this paper that bimolecular nucleophilic displacement of complexed ligand by sulfhydryl-deprotonated ligand is the major pathway for ligand exchange at physiological pH, while at the pH of the stomach the proton-assisted dissociation of the complex is the predominant means by which exchange occurs. The dynamic and equilibrium aspects of the distribution of methylmercury between chloride and sulfhydryl ligands under the solution conditions of the stomach are also considered with respect to a possible role for lipid-soluble CH3HgCl in the absorption of methylmercury from the stomach. | [
-0.025098824873566628,
0.03613527491688728,
-0.01828121393918991,
0.033888813108205795,
0.039521474391222,
-0.024335870519280434,
0.05003974959254265,
-0.01629817672073841,
-0.019468311220407486,
-0.029221059754490852,
-0.020544201135635376,
-0.09719651937484741,
0.023364894092082977,
-0.005400448106229305,
0.015034099109470844,
0.0014592304360121489,
-0.018150264397263527,
0.01591378264129162,
-0.02168060652911663,
-0.06533189117908478,
-0.03288967162370682,
0.008605388924479485,
0.011438239365816116,
-0.0285626407712698,
-0.007392091676592827,
0.03537752106785774,
-0.034204475581645966,
0.013502764515578747,
-0.09403756260871887,
-0.19623777270317078,
0.06616556644439697,
0.013702240772545338,
-0.01879424974322319,
0.01952921412885189,
-0.03933212533593178,
-0.0025703285355120897,
0.030072031542658806,
0.02975689433515072,
-0.02203633263707161,
0.036852676421403885,
0.031814929097890854,
0.047648463398218155,
0.0016888879472389817,
-0.022918563336133957,
0.004140786360949278,
0.029765702784061432,
-0.07007564604282379,
0.007782740984112024,
0.051257871091365814,
0.03401178866624832,
0.012293326668441296,
0.0018946077907457948,
-0.04041731730103493,
0.057013534009456635,
-0.003984302747994661,
0.04372018575668335,
0.05225178971886635,
-0.04034636914730072,
-0.0067465417087078094,
-0.027700185775756836,
0.05222531035542488,
-0.00606983108446002,
-0.11649397015571594,
0.0519956573843956,
0.08060578256845474,
0.015831898897886276,
0.021622641012072563,
-0.03155207633972168,
0.05787823721766472,
0.024731716141104698,
-0.04229516163468361,
0.03815869241952896,
-0.027913855388760567,
0.017242947593331337,
0.07573045045137405,
-0.04098013788461685,
0.03497292101383209,
-0.004883273039013147,
0.01833135448396206,
0.0004019337648060173,
0.018416699022054672,
-0.015554351732134819,
-0.008313482627272606,
-0.07086024433374405,
0.025399813428521156,
-0.008694003336131573,
0.03090391866862774,
-0.035675931721925735,
-0.034990955144166946,
-0.031999196857213974,
-0.02776584029197693,
-0.08618854731321335,
-0.02009105496108532,
0.016525624319911003,
-0.04082803428173065,
-0.008091879077255726,
0.04903704673051834,
0.010286682285368443,
0.035849593579769135,
0.37251925468444824,
-0.026724684983491898,
0.03876234218478203,
-0.03484373912215233,
0.0020190365612506866,
-0.03238819167017937,
0.08064116537570953,
-0.05814126878976822,
-0.028457600623369217,
-0.008778952993452549,
-0.0015265499241650105,
0.010101155377924442,
0.06041113659739494,
0.029258018359541893,
-0.04717123508453369,
0.002360768849030137,
0.035883452743291855,
-0.02910693734884262,
-0.004207940772175789,
0.0048333508893847466,
0.012609449215233326,
-0.05338994786143303,
0.02394684962928295,
0.041248295456171036,
0.025597695261240005,
0.06508923321962357,
0.0040269894525408745,
-0.02377164177596569,
0.10405748337507248,
0.01062309741973877,
0.023399053141474724,
0.036619774997234344,
-0.014080016873776913,
-0.03947867080569267,
0.01282411813735962,
-0.06080733239650726,
-0.013632719404995441,
0.034531839191913605,
-0.0112891411408782,
-0.009943035431206226,
0.009999936446547508,
-0.061363913118839264,
-0.04920889064669609,
-0.05648661032319069,
-0.14727021753787994,
-0.019813794642686844,
0.08573264628648758,
-0.031095879152417183,
-0.006405833177268505,
-0.008672496303915977,
0.026423703879117966,
-0.02108527533710003,
-0.01221452932804823,
0.004866571631282568,
0.07500221580266953,
-0.08048436045646667,
0.014998330734670162,
-0.0018553925910964608,
-0.0029735411517322063,
0.001897983136586845,
-0.014978142455220222,
0.0042352392338216305,
-0.006989666726440191,
0.023452620953321457,
0.13759592175483704,
0.020950332283973694,
0.010187163949012756,
-0.028955752030014992,
-0.05136348307132721,
0.023236814886331558,
0.004477837588638067,
0.015668874606490135,
0.02091340348124504,
0.005277327727526426,
-0.01843777485191822,
0.03190205618739128,
0.040112145245075226,
-0.008931292220950127,
0.0014018971705809236,
-0.038307927548885345,
-0.04183076322078705,
0.05958404392004013,
0.016726000234484673,
-0.06316322833299637,
0.02399856597185135,
0.09305582195520401,
0.029082778841257095,
-0.09389279037714005,
0.02312060073018074,
-0.009503251872956753,
0.060043320059776306,
-0.0037223580293357372,
-0.007992335595190525,
-0.057341013103723526,
-0.04950965940952301,
-0.06760720163583755,
-0.03921632096171379,
-0.06622626632452011,
0.0006726460997015238,
-0.06915809959173203,
0.015842121094465256,
0.10929769277572632,
0.0070948246866464615,
-0.05995439738035202,
0.052551139146089554,
0.011017546989023685,
0.0063871233724057674,
-0.015730813145637512,
0.029580658301711082,
0.0480273962020874,
-0.012204211205244064,
0.02501359023153782,
0.022152600809931755,
-0.06411507725715637,
0.006587290670722723,
-0.019350919872522354,
-0.005910263862460852,
0.05410485342144966,
0.0209555272012949,
-0.007802079897373915,
0.01239908579736948,
0.032642994076013565,
-0.05265291407704353,
-0.03926069661974907,
-0.3072035610675812,
-0.00810763705521822,
0.038765087723731995,
0.03183428943157196,
0.056593626737594604,
-0.011237271130084991,
0.015089823864400387,
-0.046957362443208694,
-0.02719498798251152,
-0.05331345275044441,
0.056738562881946564,
0.07014188170433044,
-0.047227643430233,
-0.0810714066028595,
0.009125701151788235,
0.013046331703662872,
0.016807107254862785,
-0.01640007086098194,
0.018412141129374504,
0.019543316215276718,
0.044137340039014816,
-0.06077956780791283,
-0.0003633379237726331,
0.02893782965838909,
0.10091399401426315,
-0.009043958969414234,
0.11945522576570511,
-0.004625936970114708,
-0.03642663359642029,
0.004186420701444149,
0.017169823870062828,
0.01182541809976101,
-0.0015843993751332164,
-0.07625790685415268,
-0.02085287868976593,
-0.034843992441892624,
0.01261346135288477,
-0.05525732785463333,
0.0232330821454525,
-0.020005077123641968,
-0.03994624316692352,
-0.02130597084760666,
0.00013082183431833982,
-0.03379133716225624,
-0.04279202222824097,
0.04451059177517891,
-0.034283239394426346,
-0.0047329808585345745,
0.0028758833650499582,
0.029307184740900993,
0.05675765126943588,
0.04897021874785423,
-0.013176829554140568,
-0.0054323552176356316,
-0.010095497593283653,
-0.022220255807042122,
0.05650699883699417,
0.029979286715388298,
-0.10485901683568954,
-0.01189176645129919,
-0.05394687131047249,
-0.005503417458385229,
0.05316897854208946,
0.06473850458860397,
0.006532138213515282,
-0.016229383647441864,
0.052021853625774384,
0.03150014579296112,
0.03590262308716774,
0.00823862012475729,
0.015619329176843166,
0.1274764984846115,
-0.014334991574287415,
0.011240607127547264,
0.03723393380641937,
0.03908442705869675,
-0.06568887829780579,
-0.025722341611981392,
-0.04548286646604538,
-0.02109810709953308,
0.016812480986118317,
0.014725599437952042,
0.04945908486843109,
-0.014168611727654934,
-0.02417825348675251,
0.009271204471588135,
-0.022260241210460663,
-0.047719892114400864,
0.01590266451239586,
0.018361669033765793,
0.02746611461043358,
-0.02958487719297409,
-0.06955834478139877,
-0.05115954205393791,
-0.010088562034070492,
0.02073652669787407,
-0.24501636624336243,
0.020449873059988022,
-0.005608815234154463,
0.05185540020465851,
-0.036677323281764984,
0.0027824463322758675,
0.09981308877468109,
0.011713609099388123,
-0.08848646283149719,
0.031667839735746384,
0.04230120778083801,
0.021766992285847664,
0.04979126155376434,
0.024134013801813126,
-0.00666409358382225,
0.003935983870178461,
0.0459127202630043,
-0.054507359862327576,
-0.08094553649425507,
-0.021904785186052322,
-0.01980774477124214,
-0.03676338493824005,
0.175355926156044,
0.04788736253976822,
-0.03707718849182129,
0.08240637183189392,
0.0212977584451437,
-0.0031939123291522264,
0.007850861176848412,
-0.026339827105402946,
0.04781031608581543,
0.005358328111469746,
0.056463971734046936,
0.005253988783806562,
0.035046257078647614,
0.06788107752799988,
0.014317171648144722,
-0.019146911799907684,
-0.0019767240155488253,
0.01624702289700508,
-0.0004566709976643324,
0.03709929808974266,
-0.027008526027202606,
-0.0602058582007885,
0.08645740896463394,
-0.07524775713682175,
-0.033885061740875244,
-0.05311795696616173,
0.02102537825703621,
0.010679231025278568,
-0.015486865304410458,
-0.02987518720328808,
0.006830877158790827,
-0.022700604051351547,
0.017389114946126938,
-0.03242997080087662,
0.006974675226956606,
-0.038320645689964294,
-0.0010647596791386604,
-0.012463130056858063,
0.0011653956025838852,
0.019924761727452278,
-0.029613958671689034,
0.050659552216529846,
-0.0032198240514844656
] |
PMID:25095 | A light-scattering study of the effect of calcium chloride on the molecular weight of Busycon hemocyanin. | Solutions of Busycon canaliculatum have been studied by light scattering. In 0.05 M Trizma buffer +0.1 M NaCl at pH 7.0 at 14 degrees, the weight-average molecular weight is 8.9 X 10(6). In the presence of added CaCl2 (0.02 M), the molecular weight of the protein increases to 10.7 X 10(6), and the second virial coefficient is reduced. At pH 9.95, the molecular weights with and without 0.02 M CaCl2, are 3.7 X 10(6) and 1.3 X 10(6), respectively; and the effect of Ca++ in reducing the second virial coefficient is much greater than at pH 7.0. These results can be understood on the basis that at pH 7.0, ca++ increases the association of hemocyanin, by binding and intermolecular linkage through the carboxyl groups of protein side chains. At pH 9.95, amino groups are deprotonated and therefore also become available for Ca++ binding. The relative effect of Ca++ in enhancing the association of hemocyanin therefore becomes greater at the higher pH. | A light-scattering study of the effect of calcium chloride on the molecular weight of Busycon hemocyanin. Solutions of Busycon canaliculatum have been studied by light scattering. In 0.05 M Trizma buffer +0.1 M NaCl at pH 7.0 at 14 degrees, the weight-average molecular weight is 8.9 X 10(6). In the presence of added CaCl2 (0.02 M), the molecular weight of the protein increases to 10.7 X 10(6), and the second virial coefficient is reduced. At pH 9.95, the molecular weights with and without 0.02 M CaCl2, are 3.7 X 10(6) and 1.3 X 10(6), respectively; and the effect of Ca++ in reducing the second virial coefficient is much greater than at pH 7.0. These results can be understood on the basis that at pH 7.0, ca++ increases the association of hemocyanin, by binding and intermolecular linkage through the carboxyl groups of protein side chains. At pH 9.95, amino groups are deprotonated and therefore also become available for Ca++ binding. The relative effect of Ca++ in enhancing the association of hemocyanin therefore becomes greater at the higher pH. | [
-0.126013845205307,
-0.0417400598526001,
-0.008842895738780499,
0.0023888088762760162,
0.00790361687541008,
-0.038438741117715836,
0.06852006912231445,
0.033214617520570755,
-0.058598656207323074,
0.00397875951603055,
0.051974475383758545,
-0.07381397485733032,
0.05034485086798668,
0.05334848538041115,
0.0009339938987977803,
-0.00494877714663744,
0.014491836540400982,
-0.00886470265686512,
-0.08766703307628632,
-0.019844025373458862,
-0.007358711678534746,
-0.019625777378678322,
0.015047990716993809,
0.02914218045771122,
0.01678517833352089,
0.027627689763903618,
-0.018595775589346886,
0.058142516762018204,
-0.03438754379749298,
-0.23120176792144775,
-0.018274255096912384,
0.01033847313374281,
0.02849533222615719,
-0.012114460580050945,
-0.02828589826822281,
-0.018421847373247147,
-0.04495758190751076,
-0.0395941287279129,
-0.0626668781042099,
0.038660723716020584,
0.058231648057699203,
0.03651353716850281,
-0.0022001233883202076,
0.03810017183423042,
-0.024505581706762314,
-0.03129807487130165,
0.005139938089996576,
0.014801422134041786,
0.10956892371177673,
0.02925451658666134,
-0.008826862089335918,
-0.025663960725069046,
-0.031199941411614418,
0.017901340499520302,
-0.03861096873879433,
0.005883198697119951,
0.03361694514751434,
0.012833705171942711,
-0.022380566224455833,
0.03785983845591545,
0.08233379572629929,
-0.007073938846588135,
-0.10361043363809586,
0.08677852153778076,
0.05622745677828789,
0.008026777766644955,
-0.03705829009413719,
0.016243286430835724,
0.05178051441907883,
0.08226045966148376,
-0.03632330521941185,
0.05143890157341957,
0.007758552208542824,
0.020936768501996994,
0.016561685130000114,
-0.0363631471991539,
-0.0349319651722908,
-0.008598115295171738,
-0.005586294922977686,
0.020131869241595268,
-0.006061797495931387,
0.040683284401893616,
0.033993855118751526,
-0.02965346910059452,
0.016004860401153564,
0.022115379571914673,
0.002782281953841448,
-0.021519482135772705,
-0.010403482243418694,
-0.013248199597001076,
0.026173794642090797,
-0.04808761551976204,
-0.0669315755367279,
-0.011502346955239773,
-0.09118442982435226,
0.02884608693420887,
-0.0034830747172236443,
-0.03276703879237175,
-0.022393537685275078,
0.41857537627220154,
-0.005790016148239374,
-0.004582866095006466,
-0.051856208592653275,
-0.021847618743777275,
0.02478768862783909,
0.02063950151205063,
-0.0005690054967999458,
-0.034194644540548325,
0.05495227128267288,
0.03550785407423973,
0.05324690043926239,
-0.0007733386009931564,
-0.010828372091054916,
-0.024224407970905304,
-0.009389297105371952,
0.006122566293925047,
0.03533336520195007,
0.020358648151159286,
-0.04008893296122551,
0.00721930805593729,
-0.056186359375715256,
0.0010241204872727394,
0.042699530720710754,
0.0258953720331192,
0.07640393078327179,
-0.00462369155138731,
-0.02098655328154564,
0.047775719314813614,
0.028921056538820267,
-0.004147736821323633,
0.03832964226603508,
0.026398856192827225,
-0.021385645493865013,
-0.018140701577067375,
0.00018731730233412236,
-0.005520968232303858,
0.013708220794796944,
-0.04379068687558174,
-0.008718897588551044,
0.004671676550060511,
-0.0821923315525055,
-0.04126763716340065,
-0.033215008676052094,
-0.05784749612212181,
-0.07742102444171906,
0.07896087318658829,
0.006939006503671408,
0.05697740986943245,
-0.03686651214957237,
-0.0012848031474277377,
-0.003694484243169427,
0.031673792749643326,
0.01700546219944954,
0.03148199990391731,
-0.0044134738855063915,
0.008525770157575607,
0.009908154606819153,
-0.008238419890403748,
-0.0360221192240715,
-0.01981273852288723,
-0.05757476016879082,
0.04153506085276604,
0.009694463573396206,
0.07165710628032684,
0.01920641027390957,
-0.041023142635822296,
0.017093582078814507,
-0.0070067234337329865,
-0.02308049611747265,
-0.02316165901720524,
-0.032828621566295624,
0.021841837093234062,
-0.021614888682961464,
0.011162123642861843,
0.04207063838839531,
0.027584319934248924,
-0.04300310090184212,
-0.028192762285470963,
0.025786997750401497,
-0.012271932326257229,
0.008258764632046223,
0.03687867522239685,
0.003332751337438822,
0.024844558909535408,
0.08168597519397736,
-0.054456669837236404,
-0.07791578769683838,
0.025279473513364792,
-0.055744338780641556,
0.05300111323595047,
-0.028351712971925735,
-0.024389764294028282,
-0.04880915582180023,
0.07831712067127228,
-0.06456717848777771,
-0.022672027349472046,
-0.018021676689386368,
-0.039740558713674545,
-0.0006360228289850056,
-0.010085317306220531,
0.08457143604755402,
-0.03214344382286072,
0.016307177022099495,
0.03695599362254143,
0.040308546274900436,
0.0013031878042966127,
0.02665119245648384,
0.025533948093652725,
0.02930840291082859,
-0.0017519465181976557,
0.0007611907203681767,
0.002542025176808238,
-0.06224608048796654,
-0.04674084112048149,
-0.06116896867752075,
0.042134370654821396,
-0.005530507303774357,
0.06720932573080063,
0.07034187018871307,
-0.019271844998002052,
0.040381819009780884,
-0.08469241112470627,
-0.0635242909193039,
-0.29116934537887573,
-0.01910357177257538,
0.020477408543229103,
-0.010789974592626095,
0.08697424829006195,
-0.0256524495780468,
0.02110353112220764,
0.004407197702676058,
-0.008464278653264046,
-0.05712262913584709,
0.019589420408010483,
0.043707381933927536,
0.008388626389205456,
-0.015567333437502384,
0.005489774513989687,
0.002606068504974246,
0.09976329654455185,
0.002624613232910633,
0.02834148332476616,
-0.007618861738592386,
-0.00825238972902298,
0.014625019393861294,
-0.028025012463331223,
0.029151661321520805,
0.007605439983308315,
-0.011118092574179173,
0.09235882014036179,
0.000978671363554895,
-0.061322908848524094,
0.026190780103206635,
-0.022774340584874153,
0.014572568237781525,
-0.04126863181591034,
-0.056929878890514374,
-0.02399294450879097,
0.0315844751894474,
0.019799141213297844,
-0.043208301067352295,
0.0019145408878102899,
0.012022175826132298,
-0.011535660363733768,
0.018813539296388626,
-0.00972933042794466,
-0.04032152146100998,
0.02798684686422348,
0.024612249806523323,
0.016606302931904793,
0.023384347558021545,
0.023526513949036598,
-0.014923064969480038,
0.04248548299074173,
0.05344130098819733,
0.03100188821554184,
0.04157010093331337,
0.008144154213368893,
0.020089367404580116,
-0.015888629481196404,
0.04520191624760628,
-0.05614389479160309,
-0.020079517737030983,
-0.04261764883995056,
-0.01827523298561573,
0.024569613859057426,
0.04359909147024155,
-0.03673338145017624,
0.04065769165754318,
0.04393536224961281,
-0.014421469531953335,
0.04902985692024231,
0.012940889224410057,
-0.025616556406021118,
0.06070820614695549,
-0.03281833976507187,
0.0026815365999937057,
0.03680406138300896,
0.0037947699893265963,
-0.057671383023262024,
-0.03628278896212578,
-0.009685197845101357,
0.026310525834560394,
0.060251154005527496,
-0.0029120424296706915,
0.05701975151896477,
0.009493277408182621,
-0.03903321549296379,
-0.00025319409905932844,
-0.03205801546573639,
-0.00004908401024295017,
-0.0062335217371582985,
0.026256104931235313,
-0.015045573003590107,
-0.023624712601304054,
-0.027450567111372948,
-0.004346134606748819,
0.03876083344221115,
0.06259694695472717,
-0.33078819513320923,
0.04686543345451355,
0.02026933617889881,
0.048540063202381134,
-0.014713347889482975,
0.057015009224414825,
0.05557083711028099,
-0.05275220423936844,
-0.03350817412137985,
0.035402167588472366,
0.010260263457894325,
0.011189420707523823,
0.01130610890686512,
0.02578316256403923,
0.006513293832540512,
-0.00036299641942605376,
-0.0075815655291080475,
-0.08283291012048721,
0.008303889073431492,
0.041497401893138885,
-0.0539604090154171,
-0.00934255588799715,
0.14384852349758148,
-0.045949142426252365,
-0.03921959921717644,
0.024378439411520958,
-0.02216167189180851,
0.05164970085024834,
-0.023287171497941017,
-0.010344423353672028,
0.00426723575219512,
0.08967945724725723,
0.02750023640692234,
-0.0748908743262291,
-0.004006327595561743,
0.050109200179576874,
0.017737435176968575,
-0.059442292898893356,
-0.04731862246990204,
-0.028466710820794106,
0.045750174671411514,
0.0351296067237854,
-0.08966998755931854,
-0.030757062137126923,
0.03616325184702873,
-0.014790119603276253,
0.0001903869560919702,
-0.05360805243253708,
0.014010196551680565,
-0.015508817508816719,
-0.038660500198602676,
-0.035997357219457626,
0.04294164478778839,
-0.024586791172623634,
-0.00036216803709976375,
0.0043171984143555164,
-0.024650482460856438,
0.004584398586302996,
-0.016062330454587936,
-0.0026392778381705284,
0.023718083277344704,
-0.02882823906838894,
-0.07684322446584702,
0.02422984689474106,
0.04976550489664078
] |
PMID:25096 | Effects of ammonium and bicarbonate-CO2 on intracellular chloride levels in Aplysia neurons. | The level of intracellular free chloride in Aplysia giant neurons can be made to decline by pretreatment with 50 mM NH4+ solution followed by washing with 10 mM HCO3-/0.4% CO2-containing fluids. This effect can be completely blocked by the anion flux inhibitor, 4-acetamido-4'-isothiocyano-stilbene-2,2'-disulfonic acid (SITS). The net change of free chloride in the cell cannot be explained by changes in the electrochemical gradient of chloride. These results support the hypothesis that at least one mechanism for intracellular pH regulation involves a Cl-/HCO-3 exchange process. | Effects of ammonium and bicarbonate-CO2 on intracellular chloride levels in Aplysia neurons. The level of intracellular free chloride in Aplysia giant neurons can be made to decline by pretreatment with 50 mM NH4+ solution followed by washing with 10 mM HCO3-/0.4% CO2-containing fluids. This effect can be completely blocked by the anion flux inhibitor, 4-acetamido-4'-isothiocyano-stilbene-2,2'-disulfonic acid (SITS). The net change of free chloride in the cell cannot be explained by changes in the electrochemical gradient of chloride. These results support the hypothesis that at least one mechanism for intracellular pH regulation involves a Cl-/HCO-3 exchange process. | [
-0.05565595254302025,
-0.07008600234985352,
0.02646961621940136,
0.05120028555393219,
-0.025704804807901382,
-0.01695702224969864,
0.08506853133440018,
-0.012826379388570786,
-0.06296134740114212,
-0.004737237934023142,
0.04500135779380798,
-0.09207390248775482,
-0.015537524595856667,
0.0704696848988533,
-0.0005884735146537423,
0.018643293529748917,
-0.005120677407830954,
0.0012380554107949138,
-0.05722516030073166,
0.00027755432529374957,
-0.0050288960337638855,
0.004873529076576233,
-0.031286779791116714,
-0.018136456608772278,
-0.027101662009954453,
-0.026560422033071518,
-0.010966852307319641,
-0.05073162168264389,
-0.017240291461348534,
-0.2304135113954544,
0.0008097748504951596,
0.025610629469156265,
0.014531086198985577,
-0.02898053452372551,
-0.03952686861157417,
0.025880588218569756,
0.015589463524520397,
-0.010906663723289967,
-0.02744525484740734,
0.07714878767728806,
0.02533193863928318,
0.04568403214216232,
0.016881758347153664,
0.025278793647885323,
0.006227286532521248,
-0.04094533249735832,
0.05221753567457199,
-0.027418261393904686,
0.07142380625009537,
0.013691595755517483,
0.025686923414468765,
-0.01846211589872837,
-0.010127618908882141,
0.015050445683300495,
0.004883801098912954,
0.009296601638197899,
0.008274068124592304,
0.061760492622852325,
0.04544508084654808,
-0.0042131985537707806,
0.03899049758911133,
0.06000339612364769,
-0.11908254027366638,
0.05748140066862106,
0.05552268028259277,
0.0423000231385231,
-0.04904904216527939,
0.006011234130710363,
0.06491736322641373,
0.0431305393576622,
-0.10160958766937256,
-0.006501727271825075,
0.06482592970132828,
-0.01877564564347267,
0.07252988964319229,
-0.044122207909822464,
-0.03376892954111099,
0.004549045115709305,
0.01865491457283497,
-0.030005458742380142,
-0.00034269801108166575,
0.005568031221628189,
-0.0023939546663314104,
-0.004967333283275366,
-0.004939606878906488,
0.04202868044376373,
0.024599038064479828,
-0.0001511678274255246,
-0.05083829164505005,
-0.027089234441518784,
-0.032605741173028946,
-0.05522395670413971,
-0.0048372382298111916,
-0.007351307664066553,
-0.044038452208042145,
0.05349080264568329,
0.018885301426053047,
-0.029358405619859695,
-0.03933992609381676,
0.3807617127895355,
-0.029979918152093887,
0.03405233845114708,
-0.04006826877593994,
-0.03502345085144043,
0.00962866935878992,
-0.030738310888409615,
0.004629138857126236,
0.04663645848631859,
0.036747533828020096,
0.029790177941322327,
0.027932586148381233,
0.039843011647462845,
0.0320608913898468,
-0.0001557954674353823,
-0.033338502049446106,
0.0169199388474226,
0.07048924267292023,
-0.0170099139213562,
-0.00480542192235589,
0.028228040784597397,
-0.03925498202443123,
-0.043600235134363174,
0.022250432521104813,
0.023956459015607834,
0.05638470873236656,
0.009463614784181118,
-0.00422699423506856,
0.060309767723083496,
0.01566215790808201,
-0.025518544018268585,
0.037222970277071,
0.040048833936452866,
-0.02928636036813259,
0.03826327249407768,
-0.004044021479785442,
-0.051072124391794205,
0.028410624712705612,
-0.03877083212137222,
0.008049835450947285,
0.011326600797474384,
-0.04992865398526192,
-0.03496911749243736,
-0.035725876688957214,
-0.08082818239927292,
-0.027527526021003723,
0.05498792231082916,
0.011401472613215446,
0.031409818679094315,
-0.04923337325453758,
0.023186342790722847,
-0.024069422855973244,
0.06955968588590622,
-0.025112977251410484,
0.02625739574432373,
0.03429529443383217,
-0.011226464994251728,
-0.002523852279409766,
0.005078656133264303,
-0.017084160819649696,
-0.0032224366441369057,
-0.06636537611484528,
0.05840757489204407,
-0.0005909294122830033,
0.0831945538520813,
0.028730224817991257,
0.0728115439414978,
-0.024873564019799232,
-0.05255242437124252,
-0.006216579116880894,
0.025179188698530197,
-0.015080812387168407,
0.05358446016907692,
0.0033380964305251837,
-0.08138158917427063,
0.03338610753417015,
0.03366704285144806,
-0.009603265672922134,
-0.029902465641498566,
0.03374660760164261,
-0.009076016023755074,
-0.025085512548685074,
0.004675852134823799,
0.011121095158159733,
0.03444570302963257,
0.04852507263422012,
-0.038809582591056824,
-0.07298684120178223,
0.03180106729269028,
0.0011119086993858218,
0.06345551460981369,
-0.09956931322813034,
-0.018755195662379265,
-0.11145634949207306,
0.05380634218454361,
-0.033089008182287216,
-0.046243246644735336,
-0.05728843808174133,
-0.05718552693724632,
0.008299458771944046,
-0.016650836914777756,
0.06506048142910004,
0.017290284857153893,
-0.06605087965726852,
0.02767537161707878,
0.03361012041568756,
-0.00003737836959771812,
0.020232731476426125,
-0.00984509289264679,
0.028570640832185745,
-0.014036211185157299,
-0.054541997611522675,
0.010922839865088463,
-0.038384176790714264,
0.013569248840212822,
0.017087258398532867,
0.027477266266942024,
0.003777676960453391,
0.029463961720466614,
0.05771340802311897,
-0.03542691096663475,
0.03598916530609131,
-0.053410425782203674,
-0.1159810870885849,
-0.3008976876735687,
-0.03963571414351463,
0.03285509720444679,
0.0006717384094372392,
0.0668940544128418,
-0.10586912930011749,
0.015626445412635803,
-0.026651425287127495,
0.007458720821887255,
-0.05439797788858414,
0.04394708573818207,
0.04535083472728729,
-0.041488222777843475,
-0.006011705379933119,
0.0037489053793251514,
0.01158909872174263,
0.05709530785679817,
-0.00879858247935772,
0.06430638581514359,
0.023963619023561478,
0.08023001998662949,
-0.01284811832010746,
0.047736797481775284,
0.03483188524842262,
0.008337155915796757,
-0.009847491979598999,
0.09285454452037811,
-0.04378743842244148,
0.03798737749457359,
-0.026571014896035194,
0.030585981905460358,
0.04532779008150101,
0.037571020424366,
-0.06083172932267189,
0.007627855986356735,
0.008139627054333687,
-0.031589798629283905,
-0.02047395147383213,
-0.03845306485891342,
-0.03697894141077995,
-0.04863288998603821,
-0.003647017991170287,
-0.06631406396627426,
-0.030409814789891243,
-0.023951862007379532,
0.02354271523654461,
0.0007899821503087878,
0.011489029042422771,
-0.042994216084480286,
-0.0659409835934639,
0.05230149254202843,
0.04460489749908447,
-0.031185761094093323,
-0.07306906580924988,
0.015218687243759632,
0.004570441786199808,
-0.0074231429025530815,
0.0005962826544418931,
-0.1291474550962448,
0.00007365706551354378,
-0.005662070121616125,
0.008297807537019253,
-0.011756974272429943,
-0.0009739788947626948,
0.02906728908419609,
0.032106366008520126,
0.03421715646982193,
-0.015793081372976303,
0.05401057377457619,
0.026073511689901352,
0.0638234093785286,
0.1079421415925026,
0.005317017436027527,
-0.021232984960079193,
0.03586459532380104,
0.05991485342383385,
-0.039817336946725845,
-0.039763279259204865,
-0.02801758609712124,
0.029019832611083984,
-0.017811501398682594,
-0.073454350233078,
0.048585422337055206,
0.0014691614778712392,
-0.05479072406888008,
0.05801652371883392,
-0.00018272372835781425,
-0.03721286356449127,
0.0036157185677438974,
0.012693451717495918,
-0.012752652168273926,
0.00793000403791666,
-0.048966508358716965,
-0.041517022997140884,
0.05065440014004707,
0.04454931244254112,
-0.25827279686927795,
0.0053017050959169865,
0.012572229839861393,
-0.01751803793013096,
0.043999943882226944,
0.060325101017951965,
0.015201463364064693,
-0.03055289201438427,
-0.026711897924542427,
0.04818548262119293,
0.008089953102171421,
0.018960265442728996,
0.0064169783145189285,
0.07570359855890274,
0.041636351495981216,
0.0324106328189373,
0.014621637761592865,
-0.0643584355711937,
0.05524347350001335,
-0.007114183157682419,
-0.019989967346191406,
-0.01392002496868372,
0.15644080936908722,
0.043792400509119034,
-0.002516141626983881,
0.03805733844637871,
-0.04168970137834549,
0.003738120663911104,
-0.008242486044764519,
0.02562057226896286,
-0.004456448368728161,
0.031306684017181396,
0.042657602578401566,
0.012918824329972267,
0.04369376227259636,
0.011435209773480892,
-0.03170209005475044,
-0.02512538433074951,
-0.002445481950417161,
-0.022612765431404114,
0.01642582006752491,
0.028126779943704605,
-0.08758657425642014,
-0.028915151953697205,
0.08350352197885513,
0.0030914884991943836,
0.011815163306891918,
-0.06175851821899414,
0.011280108243227005,
-0.0021025817841291428,
-0.06426537781953812,
-0.03308935835957527,
0.04811477288603783,
0.05191619321703911,
-0.02205752767622471,
-0.019310252740979195,
-0.032908834517002106,
-0.05532466247677803,
-0.02832346223294735,
-0.0038624461740255356,
0.022260965779423714,
-0.06652618944644928,
-0.004324840381741524,
0.02341064065694809,
0.03940078616142273
] |
PMID:25100 | Effects of a new selective beta1-adrenoceptor agonist on amylase secretion from the rat parotid gland. | The effects of a new selective beta1-adrenoceptor agonist, (--)-1-(4-hydroxyphenoxy)-3-isopropyl-amino-propanol-2-hydrochloride (H 133/22), on amylase secretion from the rat parotid gland were investigated in an in vitro system. The results were compared to the secretory responses obtained with noradrenaline, adrenaline, methoxyamine and terbutaline. H 133/22 was found to be a potent enzyme secretagogue and appeared even more effective than noradrenaline and adrenaline, particularly at low concentrations. The beta1-adrenoceptor agonist, terbutaline, also stimulated amylase discharge from the parotid gland but was much less potent than H 133/22. Methoxyamine had no effect on enzyme secretion. We suggest that the adrenergic stimulation of amylase secretion from the rat parotid gland is mainly mediated by beta1-receptors. | Effects of a new selective beta1-adrenoceptor agonist on amylase secretion from the rat parotid gland. The effects of a new selective beta1-adrenoceptor agonist, (--)-1-(4-hydroxyphenoxy)-3-isopropyl-amino-propanol-2-hydrochloride (H 133/22), on amylase secretion from the rat parotid gland were investigated in an in vitro system. The results were compared to the secretory responses obtained with noradrenaline, adrenaline, methoxyamine and terbutaline. H 133/22 was found to be a potent enzyme secretagogue and appeared even more effective than noradrenaline and adrenaline, particularly at low concentrations. The beta1-adrenoceptor agonist, terbutaline, also stimulated amylase discharge from the parotid gland but was much less potent than H 133/22. Methoxyamine had no effect on enzyme secretion. We suggest that the adrenergic stimulation of amylase secretion from the rat parotid gland is mainly mediated by beta1-receptors. | [
-0.028056709095835686,
-0.026178212836384773,
0.003254489740356803,
0.0435032956302166,
-0.0657564103603363,
-0.01509799249470234,
0.07210984826087952,
0.022080926224589348,
-0.04008805751800537,
-0.05159955099225044,
-0.020633848384022713,
-0.011120351031422615,
-0.040252361446619034,
0.08626295626163483,
0.011055251583456993,
0.012705901637673378,
0.05788687616586685,
0.0027331735473126173,
-0.01894201524555683,
-0.03606099635362625,
0.012617417611181736,
-0.010474265553057194,
0.0004954267060384154,
-0.003947983495891094,
-0.004376571625471115,
0.03705151379108429,
-0.02018372155725956,
0.01514127291738987,
-0.06611476093530655,
-0.17124074697494507,
0.04263438656926155,
-0.04760675132274628,
0.017998693510890007,
-0.060655925422906876,
-0.01187652163207531,
-0.0050401161424815655,
-0.0027837646193802357,
0.02956315129995346,
-0.0012636770261451602,
0.07875631749629974,
0.044975265860557556,
-0.03002358227968216,
-0.030438577756285667,
0.000899087346624583,
-0.011706585064530373,
-0.004296519793570042,
-0.0384092777967453,
-0.014008112251758575,
0.0680268406867981,
0.0144132599234581,
0.04599643871188164,
0.03444407507777214,
-0.005069424398243427,
0.03922178968787193,
0.03356704115867615,
-0.021182915195822716,
-0.030487511307001114,
0.07164628803730011,
-0.04125671088695526,
0.04696209356188774,
-0.002375614596530795,
0.02792314626276493,
-0.1600889414548874,
0.0620703361928463,
0.07782185077667236,
0.028242792934179306,
-0.04083487391471863,
-0.009709752164781094,
0.044834285974502563,
0.05516775697469711,
-0.05072112753987312,
0.0536605529487133,
-0.0022574898321181536,
-0.03929084166884422,
0.0014741135528311133,
-0.034976910799741745,
-0.0045620049349963665,
0.03699222952127457,
0.0008675147546455264,
0.010939213447272778,
0.05509565398097038,
0.01868319883942604,
0.025737620890140533,
-0.04517768695950508,
-0.013134077191352844,
-0.04971780627965927,
0.03590129315853119,
0.0033190264366567135,
-0.021585587412118912,
-0.06394640356302261,
-0.07025424391031265,
-0.055675167590379715,
-0.013997775502502918,
0.011675671674311161,
0.010159259662032127,
0.03130495920777321,
-0.0003154652949888259,
-0.04689304903149605,
-0.008047201670706272,
0.3506034016609192,
-0.025833962485194206,
0.0499831922352314,
-0.0797625184059143,
-0.03407832235097885,
-0.0008743979851715267,
-0.041313666850328445,
-0.030189257115125656,
-0.01770695485174656,
0.0009972304105758667,
0.08524749428033829,
0.06928614526987076,
-0.03782016783952713,
0.04513336345553398,
-0.04739164561033249,
-0.036732833832502365,
-0.1042090654373169,
0.012612694874405861,
-0.0076165394857525826,
-0.0029636702965945005,
0.030832266435027122,
-0.0026227415073662996,
-0.0020511371549218893,
0.000755078683141619,
0.015546250157058239,
0.0026316314470022917,
-0.02198406495153904,
-0.03340739756822586,
0.126155287027359,
0.033986981958150864,
0.021670958027243614,
0.08905395120382309,
0.04969295114278793,
-0.04181504622101784,
0.049553968012332916,
0.03889993578195572,
-0.01667899824678898,
0.031867384910583496,
0.00005883472476853058,
0.02478956989943981,
-0.008016887120902538,
0.025102486833930016,
-0.06239985302090645,
0.004881414584815502,
-0.08233818411827087,
-0.026734543964266777,
0.1287851780653,
0.05874194949865341,
0.0029191826470196247,
-0.051419537514448166,
0.027401717379689217,
-0.009205367416143417,
0.042828455567359924,
-0.06274541467428207,
0.03302482143044472,
-0.030975962057709694,
0.022278644144535065,
-0.026432182639837265,
-0.010589915327727795,
-0.004682343918830156,
0.0050586494617164135,
-0.04902692884206772,
-0.040661703795194626,
-0.011797228828072548,
-0.031147807836532593,
0.032584454864263535,
-0.04647817090153694,
-0.07192596048116684,
-0.06943359971046448,
0.019820552319288254,
0.04002567008137703,
0.017914891242980957,
-0.023342378437519073,
-0.030342379584908485,
-0.02935461513698101,
-0.09290565550327301,
0.02152862772345543,
0.07299800217151642,
0.003624140750616789,
0.026967208832502365,
-0.010570204816758633,
0.009795069694519043,
-0.03848559781908989,
0.013990282081067562,
0.0008882001857273281,
0.007078184746205807,
0.02314849942922592,
-0.010684559121727943,
0.02371056005358696,
-0.023017851635813713,
0.07240757346153259,
-0.03554120287299156,
0.021699078381061554,
-0.09604821354150772,
-0.045556891709566116,
-0.05055724456906319,
-0.0145167987793684,
-0.005723270121961832,
0.019971607252955437,
-0.03684254735708237,
0.052636176347732544,
0.04395279288291931,
0.00712443795055151,
0.009291061200201511,
0.05053803324699402,
0.012141083367168903,
0.08747081458568573,
-0.03000452183187008,
-0.03193055838346481,
0.0656709149479866,
-0.02279113046824932,
-0.025096410885453224,
-0.030592838302254677,
0.045885730534791946,
0.04238852486014366,
-0.065721295773983,
0.03172258660197258,
-0.028553789481520653,
-0.0019284184090793133,
0.0835309773683548,
-0.0074985446408391,
0.03359437361359596,
-0.005403460003435612,
0.012421865947544575,
-0.2806706130504608,
-0.06456905603408813,
-0.039650432765483856,
-0.05531212314963341,
-0.0017026894493028522,
-0.06130604073405266,
0.07152765244245529,
-0.052617013454437256,
-0.01850683055818081,
-0.050692927092313766,
0.055762939155101776,
0.004892074037343264,
0.038552623242139816,
0.014605742879211903,
0.03302835300564766,
0.07265954464673996,
0.053031112998723984,
-0.02891024388372898,
0.054266419261693954,
0.011733248829841614,
0.03797231242060661,
-0.005995625630021095,
0.009386295452713966,
-0.00960537325590849,
0.02279609441757202,
-0.05378219485282898,
0.07100578397512436,
0.02422172762453556,
-0.05204315111041069,
0.02214887924492359,
0.021482238546013832,
0.08573295176029205,
-0.047994595021009445,
-0.1246085986495018,
-0.012272323481738567,
-0.07032372802495956,
0.05632027983665466,
-0.02101132459938526,
0.0150885209441185,
-0.02298932522535324,
-0.021636173129081726,
-0.028186220675706863,
-0.016776148229837418,
-0.04117267206311226,
-0.04459746554493904,
-0.048197269439697266,
0.00979775469750166,
0.07742615789175034,
-0.029653482139110565,
0.03330579027533531,
0.04274750128388405,
0.006982592865824699,
0.036863554269075394,
0.0011909285094588995,
0.020460546016693115,
-0.03241107985377312,
-0.05133948102593422,
-0.01984792947769165,
-0.01804763451218605,
0.04000641033053398,
0.019027860835194588,
0.02457636222243309,
-0.013710965402424335,
-0.01257834117859602,
-0.022111084312200546,
0.025711072608828545,
-0.017697935923933983,
-0.028015583753585815,
0.04275795817375183,
0.026612361893057823,
0.019356954842805862,
0.11848979443311691,
-0.03251831606030464,
0.039900291711091995,
-0.0021730188746005297,
-0.010903619229793549,
0.01602061279118061,
-0.05968484282493591,
-0.09886102378368378,
0.03375731781125069,
-0.019481943920254707,
-0.020020196214318275,
0.08748575299978256,
-0.022803938016295433,
-0.052247028797864914,
0.024452747777104378,
0.049519650638103485,
0.01404731348156929,
0.053598370403051376,
0.006333422381430864,
-0.029520921409130096,
0.028271354734897614,
-0.023099754005670547,
-0.019964084029197693,
0.04919999837875366,
0.05099695920944214,
-0.2708549201488495,
0.008231387473642826,
0.03078933246433735,
0.008715789765119553,
-0.023125937208533287,
-0.0065942611545324326,
0.05677981302142143,
-0.0738844946026802,
-0.03420253098011017,
-0.013046053238213062,
0.019735019654035568,
0.03878840431571007,
0.03925264626741409,
0.08829551190137863,
-0.07399800419807434,
-0.022426141425967216,
0.017375117167830467,
0.025963569059967995,
0.0005146104958839715,
0.015116104856133461,
-0.0029104184359312057,
-0.025446977466344833,
0.12123385071754456,
-0.027142053470015526,
-0.0010871168924495578,
0.03414611518383026,
0.048747092485427856,
0.009298945777118206,
0.016317175701260567,
0.014949553646147251,
0.0032788061071187258,
0.014135236851871014,
0.05367943271994591,
-0.046824876219034195,
0.019002139568328857,
0.05137898027896881,
-0.0611196905374527,
-0.030941365286707878,
-0.03011591173708439,
0.02015804871916771,
-0.03012465499341488,
0.04708142206072807,
-0.05687759816646576,
0.001679876702837646,
0.04955701529979706,
-0.10053877532482147,
-0.06187960505485535,
0.006309788208454847,
-0.019200399518013,
-0.016686784103512764,
0.00013631665206048638,
-0.06173291802406311,
0.04607758671045303,
0.056647904217243195,
-0.0005421264795586467,
0.034009914845228195,
-0.03767264634370804,
-0.01762835867702961,
0.005288104061037302,
0.025188418105244637,
0.009521054103970528,
-0.022487370297312737,
-0.021104387938976288,
0.12687964737415314,
0.057548731565475464
] |
PMID:25117 | The effect of lysergic and diethylamide (LSD) and 2-bromolysergic acid diethylamide (BOL) on the striatal DOPA accumulation: influence of central 5-hydroxytryptaminergic pathways. | Selective chronic lesions of the dorsal raphe nucleus or combined lesions of the dorsal and median raphe nuclei did not significantly change the in vivo tyrosine hydroxylation in the striatum as measured by the DOPA accumulation after decarboxylase inhibition. Neither did acute combined lesions of the raphe nuclei, nor did electrical stimulation of the dorsal raphe nucleus have any significant effect. p-Chloroamphetamine (PCA, 20 mg/kg, i.p.) and p-chlorophenylalanine (PCPA, 400 mg/kg, i.p.), known inhibitors of the 5-hydroxytryptamine (5-HT) synthesis, significantly decreased the DOPA accumulation. The increase in DOPA accumulation observed after LSD (0.5 mg/kg, i.p.) or BOL (0.5 mg/kg, i.p.) was seemingly unaffected by pretreatment with PCA or PCPA and also after lesion of the dorsal raphe nucleus. The results suggest that the effect of LSD or BOL on the DOPA accumulation in the striatum is not mediated via a 5-hydroxytryptaminergic control mechanism originating in the dorsal raphe nucleus. A control mediated via the median raphe nucleus cannot be excluded, since LSD did not increase the DOPA accumulation after combined chronic raphe lesions. Such a control would also be in agreement with our previous results suggesting that hte DOPA generation after LSD is controlled by 5-HT receptors. | The effect of lysergic and diethylamide (LSD) and 2-bromolysergic acid diethylamide (BOL) on the striatal DOPA accumulation: influence of central 5-hydroxytryptaminergic pathways. Selective chronic lesions of the dorsal raphe nucleus or combined lesions of the dorsal and median raphe nuclei did not significantly change the in vivo tyrosine hydroxylation in the striatum as measured by the DOPA accumulation after decarboxylase inhibition. Neither did acute combined lesions of the raphe nuclei, nor did electrical stimulation of the dorsal raphe nucleus have any significant effect. p-Chloroamphetamine (PCA, 20 mg/kg, i.p.) and p-chlorophenylalanine (PCPA, 400 mg/kg, i.p.), known inhibitors of the 5-hydroxytryptamine (5-HT) synthesis, significantly decreased the DOPA accumulation. The increase in DOPA accumulation observed after LSD (0.5 mg/kg, i.p.) or BOL (0.5 mg/kg, i.p.) was seemingly unaffected by pretreatment with PCA or PCPA and also after lesion of the dorsal raphe nucleus. The results suggest that the effect of LSD or BOL on the DOPA accumulation in the striatum is not mediated via a 5-hydroxytryptaminergic control mechanism originating in the dorsal raphe nucleus. A control mediated via the median raphe nucleus cannot be excluded, since LSD did not increase the DOPA accumulation after combined chronic raphe lesions. Such a control would also be in agreement with our previous results suggesting that hte DOPA generation after LSD is controlled by 5-HT receptors. | [
0.03509138897061348,
-0.006965879816561937,
-0.0018577914452180266,
0.032034821808338165,
-0.05697094649076462,
0.05493386089801788,
0.03489901125431061,
0.01971740834414959,
-0.023050714284181595,
-0.04169725626707077,
0.07067114859819412,
-0.021335013210773468,
0.007819924503564835,
0.07101501524448395,
-0.022494740784168243,
-0.011162173934280872,
-0.02249002456665039,
-0.02293073572218418,
-0.05861489474773407,
0.00043216542690061033,
-0.017886608839035034,
-0.02380356378853321,
0.03206701949238777,
0.016112690791487694,
-0.011516904458403587,
-0.03328856825828552,
-0.04426431283354759,
-0.006703652907162905,
-0.0820913091301918,
-0.19862912595272064,
0.03564663976430893,
0.028222547844052315,
-0.009818918071687222,
-0.06435291469097137,
-0.049997150897979736,
0.029517780989408493,
-0.05617514252662659,
-0.01873023435473442,
0.04694557562470436,
0.013037309981882572,
0.019109664484858513,
0.019378703087568283,
-0.02098453789949417,
-0.04699893668293953,
-0.03391363099217415,
0.02251843549311161,
-0.028796492144465446,
-0.020622072741389275,
0.08258070051670074,
-0.003309134393930435,
-0.010521067306399345,
-0.04644513875246048,
-0.001021712669171393,
0.05440232902765274,
-0.02592623047530651,
-0.0009942101314663887,
-0.005806703120470047,
0.06033734604716301,
-0.03021707944571972,
0.011388985440135002,
-0.008985648863017559,
0.05498608946800232,
-0.17577067017555237,
0.04981184005737305,
0.04356711730360985,
-0.0069709825329482555,
-0.06659360975027084,
-0.02708408795297146,
0.06888130307197571,
0.06679538637399673,
-0.06307913362979889,
0.0026736895088106394,
0.009142028167843819,
-0.02324800007045269,
0.04788358137011528,
-0.08305623382329941,
-0.046552930027246475,
-0.0035705831833183765,
-0.006847203243523836,
-0.02561512216925621,
0.06428736448287964,
0.02455824613571167,
0.02985507622361183,
-0.01214569341391325,
-0.009689610451459885,
-0.02672356553375721,
-0.026798753067851067,
-0.05322777107357979,
-0.07248055189847946,
0.00946309044957161,
-0.0028398786671459675,
-0.09076644480228424,
0.022761041298508644,
-0.025259656831622124,
-0.057544559240341187,
0.004985099192708731,
0.014243406243622303,
-0.0024167748633772135,
0.003124028444290161,
0.3582080602645874,
-0.015434244647622108,
0.02891872264444828,
-0.07872796058654785,
0.017733700573444366,
-0.017506256699562073,
-0.04786797985434532,
0.026861082762479782,
0.015142158605158329,
-0.005885725375264883,
0.007960272021591663,
0.07537807524204254,
-0.03193933889269829,
0.06816601753234863,
-0.005173624027520418,
-0.04343593865633011,
-0.014209388755261898,
0.06643648445606232,
0.032294776290655136,
-0.0036278010811656713,
-0.002077234908938408,
-0.04441563040018082,
-0.010112415067851543,
0.09504472464323044,
0.042154859751462936,
-0.0002672337577678263,
0.003279346041381359,
0.03301670774817467,
0.09490343183279037,
0.026634173467755318,
0.007764763198792934,
0.03803829103708267,
0.006062312517315149,
-0.03068104200065136,
0.013376319780945778,
0.032839756458997726,
0.013271559961140156,
0.047639209777116776,
-0.04945085942745209,
0.004415444564074278,
0.030593261122703552,
-0.015030250884592533,
-0.03831685334444046,
0.009172551333904266,
-0.10867741703987122,
-0.0334283784031868,
0.06917489320039749,
0.046591535210609436,
-0.034818265587091446,
-0.011842782609164715,
0.04454812407493591,
-0.006861287169158459,
0.027782833203673363,
-0.029571376740932465,
0.07453367859125137,
-0.0031945600640028715,
0.03478021174669266,
0.016946803778409958,
-0.015605959109961987,
-0.0029336735606193542,
0.02740260399878025,
-0.05156309902667999,
-0.00816013291478157,
-0.004581333603709936,
0.08188420534133911,
-0.015068807639181614,
0.018045855686068535,
-0.05735054239630699,
0.0002834465994965285,
-0.03886799141764641,
0.015651706606149673,
0.01600624807178974,
0.05436043441295624,
-0.0008445012499578297,
-0.027325311675667763,
-0.032721199095249176,
-0.030026638880372047,
0.024524418637156487,
0.038025952875614166,
-0.0092441625893116,
-0.007429438177496195,
0.0335993766784668,
-0.05340501666069031,
-0.018685856834053993,
0.008566118776798248,
-0.046677641570568085,
-0.03154613450169563,
-0.012669401243329048,
-0.009335777722299099,
0.04451674595475197,
-0.016360348090529442,
-0.0037367623299360275,
-0.03684397414326668,
-0.05725501477718353,
-0.03638581931591034,
-0.01520193088799715,
-0.09917132556438446,
-0.07401949167251587,
0.009205409325659275,
-0.011624340899288654,
0.004527820739895105,
0.07560229301452637,
0.05968131497502327,
-0.04314465820789337,
0.05217625945806503,
0.0449623167514801,
0.02961372770369053,
-0.028793545439839363,
0.014011138118803501,
0.0427045002579689,
0.013462753035128117,
0.01812129095196724,
0.0039029151666909456,
0.022843772545456886,
0.05673381686210632,
-0.009179907850921154,
0.087007537484169,
-0.01780964434146881,
-0.028329363092780113,
0.028972186148166656,
-0.00782233290374279,
0.07567661255598068,
-0.05895587056875229,
-0.03003116324543953,
-0.3010750710964203,
-0.0389489121735096,
0.002910055685788393,
0.004303617402911186,
0.03279809281229973,
-0.03193215653300285,
0.005111266393214464,
-0.017712799832224846,
0.016864068806171417,
-0.002883143490180373,
-0.01403365284204483,
0.05206881836056709,
-0.03568355366587639,
-0.04967040196061134,
0.00950829591602087,
0.06283476203680038,
0.035337433218955994,
-0.02138497494161129,
0.01536571141332388,
0.034235309809446335,
0.0006785884615965188,
0.02026284858584404,
-0.004412635695189238,
-0.002888604300096631,
0.0350433811545372,
-0.04953083395957947,
0.1496184915304184,
0.044160474091768265,
-0.0042518205009400845,
-0.02829168364405632,
-0.02763068862259388,
0.0799255296587944,
-0.0026531238108873367,
-0.1312410533428192,
0.016520783305168152,
-0.01968001388013363,
0.056734077632427216,
0.008065029047429562,
-0.013695857487618923,
0.024007229134440422,
-0.0350966602563858,
0.03908565267920494,
-0.012640077620744705,
-0.08181241899728775,
-0.07595593482255936,
-0.009547035209834576,
0.027720538899302483,
-0.003621865063905716,
-0.006579518783837557,
-0.0040657189674675465,
0.04411565512418747,
-0.026463700458407402,
-0.043665919452905655,
-0.04385826364159584,
-0.015294824726879597,
-0.017618486657738686,
-0.0764942616224289,
-0.016518432646989822,
-0.03131888061761856,
-0.06914079189300537,
-0.03496516868472099,
0.005639742594212294,
0.008479036390781403,
-0.08126714080572128,
-0.006877461448311806,
0.012027794495224953,
0.024842392653226852,
0.004308377392590046,
0.09255741536617279,
0.05625848099589348,
0.0721428319811821,
0.08504091203212738,
-0.0711219534277916,
0.04894174262881279,
0.09318970888853073,
-0.019573716446757317,
-0.08225952088832855,
-0.042383525520563126,
-0.04425358399748802,
0.04581417143344879,
-0.04566947743296623,
-0.0029999478720128536,
0.03825697675347328,
0.07701662182807922,
-0.06470401585102081,
0.014272324740886688,
0.06493613868951797,
-0.052954018115997314,
0.014040004462003708,
0.008877171203494072,
0.020019501447677612,
0.0006499679875560105,
0.013114908710122108,
-0.07166416943073273,
0.022852057591080666,
0.07202856242656708,
-0.26815205812454224,
0.021853137761354446,
0.024864669889211655,
0.01369402278214693,
-0.03625945746898651,
0.08131531625986099,
0.03766581788659096,
-0.01638602837920189,
-0.03325580805540085,
0.008162216283380985,
0.004796728491783142,
0.050001274794340134,
0.055958159267902374,
0.023180590942502022,
-0.02861112169921398,
-0.013531604781746864,
0.026181969791650772,
0.005869411397725344,
0.002205905271694064,
-0.03153961896896362,
-0.02612665295600891,
-0.00043875514529645443,
0.14537671208381653,
0.021699799224734306,
-0.005565587896853685,
0.006873070728033781,
0.03996242582798004,
-0.010658088140189648,
0.0021739646326750517,
0.003516643773764372,
-0.03820106014609337,
0.006855074316263199,
0.0824783593416214,
0.007203696295619011,
0.019723379984498024,
0.04767473042011261,
-0.06279108673334122,
0.002218900714069605,
0.018503542989492416,
0.025341125205159187,
0.029020439833402634,
0.03666030243039131,
-0.04690517112612724,
0.0032544895075261593,
0.05604162812232971,
-0.03287023305892944,
-0.03808998689055443,
-0.03269347921013832,
0.002304366324096918,
0.02651246264576912,
0.0002579140418674797,
-0.014923903159797192,
-0.0014325972879305482,
0.021651538088917732,
-0.004519241396337748,
0.009120099246501923,
-0.02174435742199421,
-0.003688440890982747,
0.032558709383010864,
0.01675449311733246,
-0.012493617832660675,
0.0039998264983296394,
0.014064474031329155,
0.0789225623011589,
0.08301329612731934
] |
PMID:25118 | Monoamines, estrogen and female sexual behavior in the golden hamster. | In the golden hamster drugs which inhibit monoaminergic function (including p-chlorophenylalanine (PCPA), methysergide and a-methyl-p-tyrosine (a-MPT) facilitated lordosis in ovariectomized female hamsters as a function of the duration of estradiol benzoate (EB) priming. a-MPT (200 mg/kg), methysergide (6 mg/animal) or PCPA (150 or 360 mg/kg) potentiated lordosis if 6 days of EB priming preceded drug treatment. However, if female hamsters were primed with EB for only 2 days, a-MPT and methysergide were ineffective. PCPA (360 mg/kg) was less effective after 2 days of EB than after 6 days of EB priming. alpha-MPT produced a three-fold elevation in progesterone levels in ovariectomized females but methysergide and PCPA did not influence serum progesterone. PCPA (360 mg/kg) facilitated lordosis in adrenalectomized, ovariectomized females, eliminating the possibility that adrenal progesterone is essential for the behavioral effects of the drug. Luteinizing hormone-releasing hormone levels in the preoptic/anterior hypothalamic area and the medial basal hypothalamus were also not significantly altered at 1 h after PCPA injection. Pimozide (1.5 mg/kg) and pimozide (1.5 mg/kg) and amphetamine (2.5 mg/kg) did not potentiate lordosis in ovariectomized hamsters after either 2 or 6 days of EB priming. Pargyline, a monoamine oxidase inhibitor, inhibited female sexual behavior in females in estrogen alone-induced estrus. Lordosis in the female rat is more readily elicited both by drugs and estrogen. It is proposed with regard to female sexual behavior that species differences in estrogen sensitivity may underlie apparent differences in drug sensitivity. | Monoamines, estrogen and female sexual behavior in the golden hamster. In the golden hamster drugs which inhibit monoaminergic function (including p-chlorophenylalanine (PCPA), methysergide and a-methyl-p-tyrosine (a-MPT) facilitated lordosis in ovariectomized female hamsters as a function of the duration of estradiol benzoate (EB) priming. a-MPT (200 mg/kg), methysergide (6 mg/animal) or PCPA (150 or 360 mg/kg) potentiated lordosis if 6 days of EB priming preceded drug treatment. However, if female hamsters were primed with EB for only 2 days, a-MPT and methysergide were ineffective. PCPA (360 mg/kg) was less effective after 2 days of EB than after 6 days of EB priming. alpha-MPT produced a three-fold elevation in progesterone levels in ovariectomized females but methysergide and PCPA did not influence serum progesterone. PCPA (360 mg/kg) facilitated lordosis in adrenalectomized, ovariectomized females, eliminating the possibility that adrenal progesterone is essential for the behavioral effects of the drug. Luteinizing hormone-releasing hormone levels in the preoptic/anterior hypothalamic area and the medial basal hypothalamus were also not significantly altered at 1 h after PCPA injection. Pimozide (1.5 mg/kg) and pimozide (1.5 mg/kg) and amphetamine (2.5 mg/kg) did not potentiate lordosis in ovariectomized hamsters after either 2 or 6 days of EB priming. Pargyline, a monoamine oxidase inhibitor, inhibited female sexual behavior in females in estrogen alone-induced estrus. Lordosis in the female rat is more readily elicited both by drugs and estrogen. It is proposed with regard to female sexual behavior that species differences in estrogen sensitivity may underlie apparent differences in drug sensitivity. | [
-0.0038575069047510624,
0.022020898759365082,
0.02717527560889721,
-0.011444171890616417,
-0.008705290965735912,
0.07556139677762985,
0.06765487790107727,
0.005745094735175371,
-0.02715044468641281,
-0.014331902377307415,
0.025086067616939545,
-0.04052793234586716,
0.02043433114886284,
0.029940104112029076,
-0.005788295064121485,
0.059340499341487885,
-0.03054181858897209,
0.006169585045427084,
-0.016572820022702217,
0.020203331485390663,
-0.01585099846124649,
-0.026377221569418907,
-0.01495780423283577,
-0.04012170061469078,
0.002132842782884836,
-0.002730762818828225,
-0.009481193497776985,
-0.03795604780316353,
-0.041763585060834885,
-0.18698033690452576,
0.0022806108463555574,
-0.003308926010504365,
-0.03520316630601883,
-0.003803619183599949,
-0.03373796492815018,
0.03862879425287247,
-0.012430386617779732,
-0.0009754948550835252,
-0.046671848744153976,
0.061074502766132355,
0.04387405887246132,
-0.01704958826303482,
-0.025619100779294968,
0.015238763764500618,
0.028797313570976257,
0.02703843265771866,
-0.056853435933589935,
-0.019765522330999374,
0.046004679054021835,
-0.0522477887570858,
0.02001112885773182,
-0.023789959028363228,
-0.02154550515115261,
0.0498504713177681,
0.012856358662247658,
-0.00936012901365757,
0.008088444359600544,
0.041676364839076996,
-0.018304908648133278,
0.06213758513331413,
-0.01719941943883896,
0.07256732136011124,
-0.16107012331485748,
0.04960346594452858,
0.055696599185466766,
-0.005396625492721796,
-0.015359640121459961,
-0.03977363929152489,
0.006018985528498888,
0.058188583701848984,
-0.049217671155929565,
0.046115536242723465,
-0.001330121885985136,
-0.00735654029995203,
0.006601964123547077,
0.003991902340203524,
-0.007657729554921389,
-0.061949558556079865,
0.028525643050670624,
0.0108341621235013,
-0.010190153494477272,
0.024631377309560776,
-0.004521066322922707,
-0.04549970477819443,
0.018666349351406097,
-0.060822904109954834,
-0.008672366850078106,
-0.03758377581834793,
0.02452416718006134,
-0.022841069847345352,
-0.032006941735744476,
-0.03220044821500778,
-0.03559952974319458,
-0.06864123791456223,
-0.08896705508232117,
0.06710702925920486,
0.03305047005414963,
-0.05816150829195976,
-0.028893055394291878,
0.3489248752593994,
-0.010946061462163925,
0.06557726860046387,
-0.062018781900405884,
-0.009527278132736683,
0.03663836792111397,
0.0010429357644170523,
0.0172121599316597,
-0.014705341309309006,
-0.056129734963178635,
0.08613288402557373,
0.0688055008649826,
-0.010417327284812927,
0.06948713958263397,
-0.02239120751619339,
-0.04906652495265007,
-0.023465357720851898,
0.015722712501883507,
-0.00810148660093546,
-0.005632621701806784,
-0.024074390530586243,
-0.025701312348246574,
-0.05934037268161774,
0.06999877840280533,
0.03959148749709129,
0.037203580141067505,
0.02021639794111252,
0.009726928547024727,
0.0717020034790039,
0.013508218340575695,
-0.005817464552819729,
0.05871003866195679,
0.02875395119190216,
0.009202176705002785,
0.058837853372097015,
0.0345587432384491,
-0.0303608737885952,
0.038532860577106476,
0.00464751198887825,
0.017146790400147438,
-0.04547761753201485,
-0.03871266916394234,
-0.0829680860042572,
-0.01850588619709015,
-0.08423696458339691,
-0.014869301579892635,
0.09693874418735504,
0.08412846177816391,
-0.025392640382051468,
0.0072510577738285065,
0.039596278220415115,
0.02325841784477234,
0.0755811408162117,
-0.048100415617227554,
0.022767478600144386,
-0.031838733702898026,
0.06024489924311638,
0.010118362493813038,
-0.03283420950174332,
-0.0451933890581131,
-0.0181447621434927,
-0.06084536388516426,
0.037672821432352066,
-0.03353060409426689,
0.034251101315021515,
0.04433188587427139,
-0.025135111063718796,
-0.048920903354883194,
0.007590433582663536,
0.0063570234924554825,
0.03669264540076256,
0.002023746958002448,
0.014345463365316391,
0.006373418960720301,
-0.0239252932369709,
-0.059074901044368744,
-0.062067143619060516,
-0.003245822386816144,
0.032702986150979996,
0.017614932730793953,
0.012707841582596302,
0.0009274900075979531,
-0.04045325145125389,
-0.0243544802069664,
0.018837034702301025,
-0.0003272347676102072,
-0.006157499738037586,
-0.031182128936052322,
0.009473676793277264,
0.044046930968761444,
0.004226199816912413,
0.027432313188910484,
0.03064017742872238,
-0.04223880171775818,
-0.027931131422519684,
-0.02142518199980259,
-0.05088071897625923,
-0.08266232162714005,
0.06348608434200287,
0.0011776440078392625,
-0.005036273039877415,
0.09321975708007812,
0.06977777183055878,
0.01884528622031212,
0.05555293709039688,
0.019799649715423584,
0.056275397539138794,
0.012186176143586636,
-0.053400419652462006,
0.09951668977737427,
-0.005303685087710619,
0.0007528076530434191,
0.009800898842513561,
0.024824321269989014,
0.017104219645261765,
-0.008805348537862301,
0.1169312372803688,
0.007237493991851807,
-0.03614242002367973,
0.08919079601764679,
0.013036509975790977,
0.03665250912308693,
-0.02150334231555462,
-0.0441175140440464,
-0.30520719289779663,
-0.01688295230269432,
0.009012306109070778,
-0.047948967665433884,
0.06744123995304108,
-0.09193192422389984,
-0.004912263248115778,
-0.09243638813495636,
0.035353004932403564,
-0.04364275559782982,
0.03848831355571747,
0.057506538927555084,
-0.05126378312706947,
-0.025163181126117706,
0.008605359122157097,
0.06674432009458542,
0.04065325856208801,
-0.02166072279214859,
-0.031589165329933167,
0.013800458051264286,
-0.007661207113415003,
-0.06131714954972267,
-0.0411883108317852,
0.03371693566441536,
0.0485474057495594,
-0.013859672471880913,
0.08990459889173508,
0.037268444895744324,
-0.025525860488414764,
0.036490973085165024,
0.024828528985381126,
0.03487982973456383,
-0.014766913838684559,
-0.09058430045843124,
0.015300107188522816,
-0.025998272001743317,
0.030813105404376984,
0.0004385264473967254,
-0.031000206246972084,
-0.0038831604178994894,
-0.07966618239879608,
0.06093008816242218,
-0.013818827457726002,
-0.07046397030353546,
-0.07885607331991196,
0.014492706395685673,
0.05835752934217453,
-0.003898846684023738,
0.0069077881053090096,
-0.018388764932751656,
0.011144446209073067,
-0.00997919961810112,
-0.03958369791507721,
-0.016967903822660446,
0.0016321537550538778,
-0.009803347289562225,
-0.05025307461619377,
-0.009929576888680458,
-0.06749556958675385,
-0.0038214498199522495,
0.049508072435855865,
-0.023841992020606995,
0.0034500188194215298,
-0.08828192204236984,
-0.03913164511322975,
-0.034409645944833755,
-0.019660305231809616,
-0.01342520210891962,
0.06275548785924911,
0.08598726987838745,
-0.0005016610375605524,
0.12213040888309479,
-0.04230298474431038,
0.03522312641143799,
0.059142451733350754,
-0.021666614338755608,
-0.03360243886709213,
-0.026362579315900803,
-0.1266792118549347,
0.008168289437890053,
-0.018717555329203606,
-0.005296017043292522,
0.04108699411153793,
0.017433011904358864,
-0.03476949408650398,
-0.014307810924947262,
-0.008775018155574799,
-0.007400479167699814,
0.09178914874792099,
0.02319145016372204,
-0.04318522661924362,
0.007486552931368351,
-0.018458077684044838,
-0.017924455925822258,
0.004851386416703463,
0.07483771443367004,
-0.2626246511936188,
0.011515376158058643,
0.028438372537493706,
-0.013130087405443192,
-0.057861294597387314,
-0.0007299234275706112,
0.002664042403921485,
-0.0165011677891016,
-0.03508364409208298,
-0.01082669198513031,
0.02630334161221981,
0.07072781771421432,
0.07512315362691879,
0.03247538208961487,
-0.03518391400575638,
0.020578397437930107,
0.0012372304918244481,
0.07171422988176346,
-0.0053220209665596485,
-0.0906984731554985,
0.0092654749751091,
-0.07464508712291718,
0.11742108315229416,
0.018560415133833885,
0.003483089152723551,
-0.018582526594400406,
0.030510632321238518,
0.03053799830377102,
0.012881658971309662,
-0.0345124825835228,
-0.023953169584274292,
0.03813107684254646,
0.07890308648347855,
0.03696981817483902,
0.05026334896683693,
0.031031329184770584,
-0.049382731318473816,
0.014934894628822803,
0.0011382964439690113,
0.005524273496121168,
-0.025261910632252693,
0.039967700839042664,
-0.04740036651492119,
0.014088835567235947,
0.06739172339439392,
-0.06458071619272232,
-0.014614349231123924,
-0.035965342074632645,
-0.003053383668884635,
0.03277783468365669,
0.020278021693229675,
-0.0012465061154216528,
0.008833217434585094,
0.02123887650668621,
-0.022008510306477547,
-0.0439576730132103,
0.020107189193367958,
0.034589558839797974,
0.0040193647146224976,
0.007392782252281904,
-0.016750285401940346,
0.006763517390936613,
-0.06371871381998062,
0.09415105730295181,
0.02415536344051361
] |
PMID:25121 | Synaptic connexions and related postsynaptic pharmacology studied in the cerebral ganglion of Aplysia. | Interneuronal connexions have been studied within the cerebral ganglion of Aplysia californica. Excitatory monosynaptic connexions between two groups of cells located on the dorsal surface of the ganglion near the cerebropleural connectives were analyzed in detail. The monosynapticity of these connexions was established not only by the strict one-to-one correlation between presynaptic action potential and excitatory postsynaptic response (EPSP) and the constant latency for any given cell pair, but also by the following criteria: (a) gradual change in the EPSP following tetraethylammonium injection into the presynaptic neurone, (b) sustained EPSP in the presence of a high external calcium ion concentration, (c) sensitivity of the EPSP amplitude to presynaptic polarization. Iontophoretic application of acetylcholine, 5-hydroxytryptamine and glutamate on the postsynaptic cells elicited excitatory responses in many cases. Inhibitory responses were obtained by local iontophoresis of dopamine, gamma-aminobutyric acid and occasionally also by acetylcholine. The only agent found to block the EPSP was bufotenine, which also readily blocked the 5-hydroxytryptamine response. Bufotenine was completely ineffective on the acetylcholine or glutamate excitatory responses. Of the various cholinolytics tested, none had an effect on the EPSP. Our data all point to 5-hydroxytryptamine as a transmitter in the studied synaptic connexions. However, it must be emphasized that in the absence of biochemical and histological evidence the role of the 5-hydroxytryptamine cannot be regarded as conclusive. | Synaptic connexions and related postsynaptic pharmacology studied in the cerebral ganglion of Aplysia. Interneuronal connexions have been studied within the cerebral ganglion of Aplysia californica. Excitatory monosynaptic connexions between two groups of cells located on the dorsal surface of the ganglion near the cerebropleural connectives were analyzed in detail. The monosynapticity of these connexions was established not only by the strict one-to-one correlation between presynaptic action potential and excitatory postsynaptic response (EPSP) and the constant latency for any given cell pair, but also by the following criteria: (a) gradual change in the EPSP following tetraethylammonium injection into the presynaptic neurone, (b) sustained EPSP in the presence of a high external calcium ion concentration, (c) sensitivity of the EPSP amplitude to presynaptic polarization. Iontophoretic application of acetylcholine, 5-hydroxytryptamine and glutamate on the postsynaptic cells elicited excitatory responses in many cases. Inhibitory responses were obtained by local iontophoresis of dopamine, gamma-aminobutyric acid and occasionally also by acetylcholine. The only agent found to block the EPSP was bufotenine, which also readily blocked the 5-hydroxytryptamine response. Bufotenine was completely ineffective on the acetylcholine or glutamate excitatory responses. Of the various cholinolytics tested, none had an effect on the EPSP. Our data all point to 5-hydroxytryptamine as a transmitter in the studied synaptic connexions. However, it must be emphasized that in the absence of biochemical and histological evidence the role of the 5-hydroxytryptamine cannot be regarded as conclusive. | [
-0.011499054729938507,
-0.054264433681964874,
-0.004506601020693779,
-0.03569959104061127,
-0.041741564869880676,
0.02030317299067974,
0.08437254279851913,
0.03841831535100937,
0.0017758827889338136,
-0.06747949123382568,
0.05388239026069641,
-0.08129248768091202,
-0.006161757279187441,
0.04518565908074379,
0.029867224395275116,
0.017815444618463516,
-0.014199953526258469,
-0.02061091735959053,
-0.009265434928238392,
-0.0037437058053910732,
-0.005375642329454422,
-0.043702464550733566,
-0.004490851890295744,
-0.02522646263241768,
0.0011016246862709522,
-0.013987475074827671,
-0.001486341468989849,
-0.023971902206540108,
-0.03086835891008377,
-0.19849085807800293,
-0.0558113195002079,
0.0034870330709964037,
-0.03673030808568001,
-0.08594303578138351,
-0.050767119973897934,
-0.00013316066178958863,
0.02318601682782173,
-0.02301545813679695,
-0.02984924614429474,
0.043777164071798325,
-0.021185478195548058,
0.02518177218735218,
-0.022436391562223434,
0.017814533784985542,
-0.008954600431025028,
-0.04565328732132912,
-0.015199749730527401,
-0.036534376442432404,
0.07272721827030182,
-0.048786770552396774,
0.021017132326960564,
-0.03175140544772148,
0.04218851029872894,
0.040040887892246246,
-0.018247976899147034,
0.015129647217690945,
-0.011467330157756805,
0.030464384704828262,
-0.024914881214499474,
0.03971406817436218,
0.04168730229139328,
0.07485862821340561,
-0.12386006116867065,
0.07271235436201096,
0.07778674364089966,
0.0010003035422414541,
-0.004050133749842644,
0.0030516795814037323,
0.05531229078769684,
0.06288877129554749,
-0.05555378273129463,
0.025078464299440384,
-0.0198599211871624,
-0.0074562206864356995,
0.046275049448013306,
-0.02482173778116703,
-0.007889715023338795,
-0.007383138407021761,
0.04579775035381317,
0.01289078313857317,
0.06836282461881638,
-0.008456814102828503,
0.008269989863038063,
-0.030076228082180023,
0.022974150255322456,
0.005275631323456764,
0.04738687723875046,
0.007678970694541931,
-0.025894204154610634,
0.017719656229019165,
-0.029972372576594353,
-0.034246210008859634,
-0.03704747185111046,
-0.03933846205472946,
-0.02768808975815773,
0.05994056537747383,
0.016926415264606476,
-0.029094310477375984,
0.007096836809068918,
0.3876555860042572,
-0.04425238072872162,
0.032491039484739304,
-0.03506879508495331,
-0.024219196289777756,
-0.009967923164367676,
0.014189065434038639,
-0.009281275793910027,
-0.032898031175136566,
0.011252567172050476,
0.06417128443717957,
0.03659874200820923,
-0.011927401646971703,
0.057221315801143646,
0.016246354207396507,
-0.03233050927519798,
-0.042023975402116776,
0.04662824422121048,
-0.0293254591524601,
0.02208309806883335,
0.0005145283648744226,
-0.04232040420174599,
-0.012111116200685501,
0.04050804674625397,
0.025284668430685997,
0.08028948307037354,
-0.03004610165953636,
-0.0931352749466896,
0.025151720270514488,
0.04710471257567406,
-0.022706683725118637,
0.05297819525003433,
0.02532048150897026,
0.043486595153808594,
0.01160308625549078,
0.011914946138858795,
0.010830572806298733,
0.08072283118963242,
-0.04478225111961365,
-0.010202272795140743,
-0.03853350132703781,
-0.007332222070544958,
-0.04822232201695442,
0.02457665279507637,
-0.05172176659107208,
-0.040855757892131805,
0.10754425823688507,
0.030955804511904716,
0.015350615605711937,
-0.020108645781874657,
0.04876888915896416,
-0.02093774825334549,
0.018241465091705322,
-0.005832147318869829,
0.03512132912874222,
-0.009116788394749165,
0.06476247310638428,
0.0006795746157877147,
-0.02265792153775692,
-0.011873159557580948,
-0.004977609496563673,
-0.02945959009230137,
0.021655475720763206,
0.011555163189768791,
0.09199830889701843,
0.034804243594408035,
0.0012916810810565948,
-0.05588718503713608,
-0.016987761482596397,
-0.045402489602565765,
0.010492268018424511,
-0.01907457783818245,
0.011157161556184292,
0.013619833625853062,
-0.023629555478692055,
-0.0817139521241188,
-0.006230968050658703,
0.004132057540118694,
-0.016891321167349815,
0.02499745599925518,
-0.021178700029850006,
-0.025190474465489388,
-0.05357301980257034,
-0.005022476892918348,
-0.008271923288702965,
-0.005201532505452633,
-0.008816037327051163,
-0.023167382925748825,
0.022503534331917763,
0.028024185448884964,
0.00032895136973820627,
0.01585315726697445,
-0.009469946846365929,
-0.05024866759777069,
0.04447360709309578,
-0.0449131540954113,
-0.036655355244874954,
-0.014698097482323647,
-0.025618866086006165,
0.012194079346954823,
0.00585166597738862,
0.05806213617324829,
0.014671419747173786,
-0.04614297300577164,
0.06623070687055588,
0.041784945875406265,
0.0016057025641202927,
-0.009971222840249538,
0.04640991985797882,
0.03766167536377907,
-0.028906045481562614,
-0.031801436096429825,
-0.013215742073953152,
0.019452862441539764,
-0.015509435907006264,
-0.02368343062698841,
0.06486786156892776,
-0.05339931324124336,
0.0685579776763916,
0.07088769227266312,
-0.026218194514513016,
0.016307810321450233,
-0.02211005985736847,
-0.03325474262237549,
-0.2910914123058319,
-0.048322781920433044,
0.014348160475492477,
0.010339313186705112,
0.05172133445739746,
-0.06684612482786179,
0.013634887523949146,
-0.03481816500425339,
0.02963699959218502,
-0.07305954396724701,
0.02513028122484684,
0.07332489639520645,
0.0009865890024229884,
0.0024601782206445932,
0.04533621668815613,
0.0948510468006134,
0.07637430727481842,
-0.061163149774074554,
0.0617145374417305,
0.004199688788503408,
0.020731179043650627,
0.006300127133727074,
-0.04269716516137123,
0.03984491899609566,
0.02692626602947712,
-0.04942341148853302,
0.1067596822977066,
0.011477653868496418,
-0.013951094821095467,
-0.0499710887670517,
-0.02211390994489193,
0.07221691310405731,
-0.00960374902933836,
-0.09329627454280853,
0.002619280479848385,
-0.004467998165637255,
0.007827001623809338,
0.024394245818257332,
-0.017722342163324356,
-0.027400953695178032,
-0.0772157609462738,
0.07643751055002213,
-0.025706971064209938,
-0.06361337751150131,
-0.06250359117984772,
-0.024681363254785538,
0.00556205352768302,
-0.03199383243918419,
-0.002310726325958967,
-0.047514501959085464,
0.04522671550512314,
0.029839588329195976,
0.005433883983641863,
-0.042610540986061096,
-0.0039010271430015564,
0.02865701913833618,
-0.08239524811506271,
0.008960610255599022,
-0.08140873163938522,
0.0035498777870088816,
0.021761365234851837,
0.005642932374030352,
-0.0003779674007091671,
-0.01671125367283821,
0.021690411493182182,
0.07604674249887466,
-0.005621389020234346,
-0.057195547968149185,
0.08620008081197739,
0.041679494082927704,
0.040306154638528824,
0.0961124449968338,
-0.004637763369828463,
0.05280816927552223,
0.0448673851788044,
-0.010646316222846508,
0.03701505810022354,
-0.04240981489419937,
-0.07422122359275818,
0.06868653744459152,
-0.03264083340764046,
-0.04119265079498291,
0.02649207040667534,
0.010731611400842667,
-0.09875816106796265,
-0.020357729867100716,
0.052006371319293976,
-0.026418114081025124,
0.08235924690961838,
0.011657745577394962,
-0.031468722969293594,
0.01460321806371212,
-0.018245158717036247,
-0.055516231805086136,
0.026557618752121925,
0.11473056674003601,
-0.2656647562980652,
0.06812271475791931,
0.04894062876701355,
0.005577995907515287,
-0.037266988307237625,
0.07574175298213959,
0.001514896983280778,
-0.07700652629137039,
-0.01779680885374546,
0.03991125524044037,
-0.017974326387047768,
0.041266974061727524,
0.03931426629424095,
0.02239091694355011,
-0.02220657654106617,
0.04203345626592636,
0.035378679633140564,
-0.013474654406309128,
0.07064589112997055,
0.0038534116465598345,
-0.03488674387335777,
-0.019947154447436333,
0.14442743360996246,
0.03933422267436981,
-0.008120075799524784,
0.00040579563938081264,
-0.006810187827795744,
0.004261487629264593,
-0.01946050301194191,
-0.01161206141114235,
0.003945528995245695,
0.02718462236225605,
0.06963822990655899,
-0.08705517649650574,
0.04409138485789299,
0.0035959442611783743,
-0.053701963275671005,
-0.02229141816496849,
0.014465195126831532,
-0.05969397351145744,
-0.015744997188448906,
0.01794811151921749,
-0.023042181506752968,
-0.020463448017835617,
0.0776919573545456,
-0.025038864463567734,
-0.05688915774226189,
-0.023593343794345856,
-0.0232456736266613,
-0.013161873444914818,
-0.015751127153635025,
-0.06027887761592865,
-0.005959684960544109,
0.0352763906121254,
-0.017258407548069954,
-0.015235180966556072,
-0.06423457711935043,
0.0022169845178723335,
0.03863667696714401,
0.013862816616892815,
-0.02399221621453762,
-0.060702934861183167,
-0.01043635606765747,
0.06124710291624069,
0.04932299256324768
] |
PMID:25128 | Effects of pH and flow rate on the release of 'bound' red cells from the splenic pulp. | On perfusion of isolated, denervated spleens with Ringer solution, immature and abnormal red cells are released into the venous outflow much more slowly than normal mature cells, being delayed through adherence to fine structures of the red pulp (Am. J. Physiol. 231, 1665-1671 (1976)). Evidence suggested that the rate at which such cells are released from the 'bound' state might depend on local pH and fluid shear rate within the pulp. Therefore, the rate of washout for this slow component of red cells, from cat spleens, was measured as a function of pH and flow rate of the perfusate. The volume of solution (V 1/2) for 50% washout of 'bound' cells decreased as pH was lowered from 7.8 to 6.6, especially (from 97 to 18 ml/g) between 7.4 and 6.6. The percentage total red cell outflow thus represented rose from 0.06 to 0.5 as pH fell from 7.8 to 6.6. At a high perfusion rate (14-16 ml/min) the V 1/2 value was only one-half that prevailing at a lower rate (4-6 ml/min), and the percentage flow of 'bound' red cells was more than three times greater. Both acidic pH and augmented blood flow thus assist release of adherent red cells from the splenic pulp. | Effects of pH and flow rate on the release of 'bound' red cells from the splenic pulp. On perfusion of isolated, denervated spleens with Ringer solution, immature and abnormal red cells are released into the venous outflow much more slowly than normal mature cells, being delayed through adherence to fine structures of the red pulp (Am. J. Physiol. 231, 1665-1671 (1976)). Evidence suggested that the rate at which such cells are released from the 'bound' state might depend on local pH and fluid shear rate within the pulp. Therefore, the rate of washout for this slow component of red cells, from cat spleens, was measured as a function of pH and flow rate of the perfusate. The volume of solution (V 1/2) for 50% washout of 'bound' cells decreased as pH was lowered from 7.8 to 6.6, especially (from 97 to 18 ml/g) between 7.4 and 6.6. The percentage total red cell outflow thus represented rose from 0.06 to 0.5 as pH fell from 7.8 to 6.6. At a high perfusion rate (14-16 ml/min) the V 1/2 value was only one-half that prevailing at a lower rate (4-6 ml/min), and the percentage flow of 'bound' red cells was more than three times greater. Both acidic pH and augmented blood flow thus assist release of adherent red cells from the splenic pulp. | [
-0.05950579792261124,
-0.020292067900300026,
0.005017437972128391,
0.021028146147727966,
0.024004684761166573,
-0.030439503490924835,
0.06211673840880394,
0.05221439152956009,
0.041033338755369186,
0.02618625946342945,
0.033211637288331985,
-0.06028394028544426,
0.011077472008764744,
0.031138943508267403,
-0.06834398210048676,
0.0008766362443566322,
-0.008946257643401623,
0.00747864181175828,
-0.12940286099910736,
0.018290851265192032,
-0.03216579556465149,
-0.019619250670075417,
-0.00937657617032528,
-0.04935735464096069,
0.04522906616330147,
-0.027805058285593987,
-0.016447562724351883,
-0.062076907604932785,
-0.04466793313622475,
-0.21210305392742157,
-0.02110065147280693,
0.0010857292218133807,
0.00976486410945654,
0.006253987550735474,
-0.025778500363230705,
0.01967235654592514,
-0.0253676176071167,
-0.016591772437095642,
-0.039187539368867874,
0.03408284857869148,
0.012591799721121788,
0.05035443976521492,
0.016009263694286346,
0.05149180814623833,
0.031195206567645073,
0.07840096205472946,
-0.014605500735342503,
-0.009547222405672073,
0.0849008560180664,
0.06835346668958664,
0.014503942802548409,
-0.004176800604909658,
-0.0006218731869012117,
0.039312802255153656,
-0.008352342061698437,
-0.013868100009858608,
-0.01811087317764759,
0.0430198572576046,
0.05743589624762535,
-0.004137663636356592,
-0.012778491713106632,
0.003252517431974411,
-0.13828468322753906,
0.07706589996814728,
0.0762450322508812,
-0.05544945225119591,
-0.07735513150691986,
0.010632680729031563,
0.05643001198768616,
0.018676936626434326,
-0.07136432826519012,
0.04262585565447807,
-0.02306426502764225,
0.020004943013191223,
0.01903967745602131,
-0.027292104437947273,
0.008517236448824406,
-0.03242949768900871,
0.01611432433128357,
0.06486961990594864,
0.0006374834920279682,
-0.021893681958317757,
-0.022461911663413048,
-0.023959003388881683,
-0.007006961852312088,
-0.018362974748015404,
0.0034758939873427153,
-0.05384533479809761,
-0.048758264631032944,
0.009472007863223553,
-0.02145853452384472,
-0.005318350158631802,
-0.02314966730773449,
0.04510834068059921,
-0.08562722057104111,
0.029396994039416313,
-0.02034134417772293,
-0.0008969329646788538,
-0.004149722401052713,
0.4079713225364685,
-0.04185643792152405,
0.025269892066717148,
-0.003725707298144698,
-0.00772114796563983,
0.03153315931558609,
-0.01644059829413891,
0.005142981186509132,
0.006385075394064188,
-0.04288778454065323,
0.0409366674721241,
0.017843779176473618,
0.016670087352395058,
0.022354189306497574,
-0.0005812704330310225,
0.004767723847180605,
0.01744030974805355,
0.01991976611316204,
-0.01016198843717575,
-0.006265907548367977,
0.0067155505530536175,
-0.030453702434897423,
0.025732731446623802,
0.008644092828035355,
-0.02601400949060917,
0.08924231678247452,
0.012984623201191425,
0.006597410887479782,
0.07460848242044449,
0.0351385697722435,
0.018340812996029854,
0.05014742538332939,
0.018271001055836678,
-0.03229774534702301,
-0.015126299113035202,
-0.048740848898887634,
-0.046492334455251694,
0.05216721072793007,
0.02001205086708069,
0.014422454871237278,
-0.04176522046327591,
-0.05225740373134613,
-0.06356975436210632,
-0.034528061747550964,
-0.07262948155403137,
-0.061545271426439285,
0.09209050983190536,
0.009837820194661617,
0.042370181530714035,
-0.026800690218806267,
-0.041625358164310455,
-0.03595026582479477,
0.040544409304857254,
-0.010876297950744629,
0.005127756856381893,
-0.028303228318691254,
-0.01680022105574608,
-0.044364359229803085,
-0.01325173582881689,
-0.008798344060778618,
0.007119814865291119,
-0.028741497546434402,
-0.0286514051258564,
-0.011687100864946842,
0.05816255882382393,
-0.014138949103653431,
0.03829783573746681,
0.007139217108488083,
-0.0848148986697197,
0.034415073692798615,
0.006830022670328617,
-0.010020347312092781,
-0.023993780836462975,
0.01595042273402214,
-0.07460662722587585,
-0.0031397745478898287,
0.029826167970895767,
0.0031583430245518684,
-0.018452446907758713,
0.06345715373754501,
-0.016511274501681328,
0.09314722567796707,
0.06239455193281174,
0.004871692042797804,
0.02309371344745159,
0.012630680575966835,
-0.07468713074922562,
-0.09827905148267746,
-0.00939227920025587,
0.00547430757433176,
0.06684843450784683,
-0.03787601366639137,
-0.02801954746246338,
-0.027394499629735947,
-0.00032110896427184343,
-0.021825959905982018,
-0.035291895270347595,
-0.02755558490753174,
-0.035200707614421844,
-0.07138628512620926,
0.006579310167580843,
0.044417303055524826,
-0.02254217118024826,
-0.045182593166828156,
0.02915261499583721,
0.028602154925465584,
0.06063332408666611,
0.04530889913439751,
0.011042243801057339,
-0.004817725624889135,
0.03587978705763817,
-0.039455778896808624,
0.0067079756408929825,
0.007029544562101364,
-0.012109586969017982,
-0.04844985529780388,
0.05647166445851326,
0.04528714343905449,
-0.008061722852289677,
0.02655520662665367,
-0.008367921225726604,
0.06693603843450546,
-0.08508943021297455,
-0.019118594005703926,
-0.2849878966808319,
0.025097250938415527,
-0.005558977834880352,
-0.02384197525680065,
0.07195621728897095,
0.002417933661490679,
0.023188836872577667,
-0.015449008904397488,
0.031977467238903046,
-0.04143747314810753,
0.03636585548520088,
0.06551012396812439,
-0.04749531298875809,
-0.044242702424526215,
0.07129520177841187,
0.02057707868516445,
0.022570744156837463,
0.023681331425905228,
0.07404889166355133,
0.0368681401014328,
0.06209241598844528,
0.0009445373434573412,
0.004281559027731419,
0.03171393647789955,
0.030178118497133255,
-0.005221465602517128,
0.11611475050449371,
-0.032013729214668274,
-0.020144877955317497,
-0.07489974796772003,
-0.016201529651880264,
0.040317945182323456,
0.01011759415268898,
-0.02093980647623539,
-0.06925665587186813,
-0.035414449870586395,
0.029955679550766945,
-0.016291603446006775,
0.025698164477944374,
0.018727220594882965,
-0.05500071868300438,
0.01428989041596651,
-0.03884051740169525,
-0.04567735269665718,
-0.01473032683134079,
0.008021024987101555,
0.02209501713514328,
0.03416245058178902,
-0.05523447319865227,
-0.004199670162051916,
0.04404769837856293,
-0.061955247074365616,
0.03337825462222099,
-0.04131900146603584,
0.0942094624042511,
-0.014738308265805244,
0.0025296404492110014,
0.057723358273506165,
-0.09978341311216354,
-0.007283915299922228,
-0.060693539679050446,
-0.02832142636179924,
0.04499625414609909,
-0.01462281309068203,
-0.020198270678520203,
-0.0007157478830777109,
0.023652000352740288,
0.02346630208194256,
0.051965028047561646,
-0.01277091633528471,
0.01498590037226677,
0.10588060319423676,
0.009410936385393143,
0.05765432491898537,
0.0227112527936697,
0.039263151586055756,
-0.029722414910793304,
-0.0010655440855771303,
-0.01132828276604414,
-0.026701120659708977,
0.06558682024478912,
-0.014475898817181587,
0.07273440062999725,
0.01930692046880722,
-0.022359488531947136,
0.03493937849998474,
0.00583734130486846,
-0.017080528661608696,
-0.0401562936604023,
-0.017307059839367867,
-0.02289430983364582,
0.03769136220216751,
0.013461019843816757,
-0.03634149208664894,
0.008830983191728592,
-0.00019041856285184622,
-0.2801854610443115,
0.020324138924479485,
0.04657041281461716,
-0.05673570558428764,
-0.02965783141553402,
0.03547634556889534,
0.07171972841024399,
-0.026613829657435417,
0.012697224505245686,
0.026866665109992027,
0.0048787458799779415,
0.0005762765649706125,
0.0077969073317945,
0.01775587722659111,
-0.0023645740002393723,
-0.048157334327697754,
0.031197603791952133,
-0.04638288915157318,
-0.00808507390320301,
-0.03502405807375908,
0.028713157400488853,
-0.034523166716098785,
0.16078706085681915,
-0.03736750781536102,
-0.0369148850440979,
-0.005380734801292419,
-0.004507753998041153,
0.048951271921396255,
-0.002033079508692026,
-0.009945336729288101,
0.04634367674589157,
0.02740289643406868,
0.035913340747356415,
0.02431398816406727,
-0.006030089221894741,
-0.01361859031021595,
-0.0250667966902256,
0.039513759315013885,
-0.026556814089417458,
0.028154442086815834,
0.06060871109366417,
0.0012299302034080029,
-0.037588123232126236,
-0.04930697754025459,
0.05047241970896721,
0.010568943805992603,
-0.02939324453473091,
-0.0877477303147316,
0.02695889212191105,
-0.021632753312587738,
-0.020059479400515556,
-0.07541760057210922,
0.05343011021614075,
0.030091773718595505,
0.03850604221224785,
0.020760713145136833,
0.012273697182536125,
-0.056299831718206406,
0.006880809552967548,
-0.006190910469740629,
0.020512210205197334,
-0.08601635694503784,
0.012709462083876133,
0.0787382498383522,
0.005333512090146542
] |
PMID:25129 | An alkaline phosphatase mutant of Pseudomonas aeruginosa. 1. Effects of regulatory, structural, and environmental shifts on enzyme function. | An alkaline phosphatase mutant of Pseudomonas aeruginosa exhibiting both regulatory and catalytic changes was isolated. Under repression conditions (i.e. high inorganic phosphate (Pi)) the mutant culture produced an alkaline phosphatase (APase) displaying significant activity against both beta-glycerol phosphate (betaGP) and p-nitrophenyl phosphate (pNPP), while the wild type displayed no activity directed towards these substrates under the same conditions. In vivo, the mutant enzyme's ratio of specific activities was 45:1 in favour of betaGP versus pNPP, whereas this ratio was reversed to 1:9 betaGP versus pNPP for the same enzyme isolated from mutant cells. In addition, the kinetic parameters and stability requirements for the mutant-derived enzyme was altered in comparison with those of the wild type. A study of lipopolysaccharide (LPS) preparations from both the mutant and wild type indicated the mutant to be deficient in the core region of its LPS. The authors propose that the modifications in the catalytic activity of the mutant enzyme, demonstrated in vivo, are due to a change in the enzyme's microenvironment. | An alkaline phosphatase mutant of Pseudomonas aeruginosa. 1. Effects of regulatory, structural, and environmental shifts on enzyme function. An alkaline phosphatase mutant of Pseudomonas aeruginosa exhibiting both regulatory and catalytic changes was isolated. Under repression conditions (i.e. high inorganic phosphate (Pi)) the mutant culture produced an alkaline phosphatase (APase) displaying significant activity against both beta-glycerol phosphate (betaGP) and p-nitrophenyl phosphate (pNPP), while the wild type displayed no activity directed towards these substrates under the same conditions. In vivo, the mutant enzyme's ratio of specific activities was 45:1 in favour of betaGP versus pNPP, whereas this ratio was reversed to 1:9 betaGP versus pNPP for the same enzyme isolated from mutant cells. In addition, the kinetic parameters and stability requirements for the mutant-derived enzyme was altered in comparison with those of the wild type. A study of lipopolysaccharide (LPS) preparations from both the mutant and wild type indicated the mutant to be deficient in the core region of its LPS. The authors propose that the modifications in the catalytic activity of the mutant enzyme, demonstrated in vivo, are due to a change in the enzyme's microenvironment. | [
-0.060559533536434174,
0.02999946102499962,
-0.03898540511727333,
0.036573171615600586,
-0.013815479353070259,
-0.07487352192401886,
0.015771327540278435,
0.028420204296708107,
-0.05813479423522949,
-0.0005130756762810051,
0.0005273286951705813,
-0.08546353876590729,
0.010648875497281551,
0.005954561289399862,
-0.011584482155740261,
-0.05046882852911949,
-0.029640953987836838,
-0.021754557266831398,
-0.017615625634789467,
0.03302926570177078,
0.017069494351744652,
0.022564783692359924,
0.023266183212399483,
0.003136353101581335,
0.03356090188026428,
0.04642543941736221,
0.03014189377427101,
0.06135684251785278,
-0.060303229838609695,
-0.2148134708404541,
-0.04744117707014084,
0.00013139091606717557,
0.02840252034366131,
-0.08227767050266266,
-0.011009384877979755,
0.036379411816596985,
0.008219010196626186,
0.012659470550715923,
-0.016123410314321518,
0.04994804784655571,
0.0504249669611454,
0.017461014911532402,
0.004161842633038759,
0.03629566356539726,
0.0003606174432206899,
-0.010420022532343864,
0.004466596525162458,
0.004746466409415007,
0.06719175726175308,
-0.0593164786696434,
-0.03192564472556114,
-0.010086890310049057,
0.003997182007879019,
0.028512869030237198,
0.03495365381240845,
-0.020068632438778877,
0.05764609947800636,
0.06412636488676071,
0.011831299401819706,
-0.028660623356699944,
0.011675351299345493,
-0.04279819130897522,
-0.1387307494878769,
0.054145004600286484,
0.043330658227205276,
0.03080250509083271,
-0.012395738624036312,
-0.032296646386384964,
0.04111083224415779,
0.0513528510928154,
-0.0875934436917305,
0.05427904427051544,
-0.0014470294117927551,
-0.03179850056767464,
0.020747704431414604,
0.03594256937503815,
-0.03666610270738602,
0.02386140078306198,
0.047839414328336716,
0.021659407764673233,
0.009163806214928627,
-0.03396185487508774,
-0.02384759858250618,
0.019276851788163185,
-0.04300481081008911,
-0.017313016578555107,
0.08690609782934189,
-0.014349473640322685,
0.01745428331196308,
0.050279080867767334,
-0.01803160458803177,
-0.04176424816250801,
0.05366048589348793,
-0.03662490099668503,
-0.032858431339263916,
0.08913657069206238,
0.0344504714012146,
0.006751321721822023,
0.006878299172967672,
0.3747783303260803,
-0.06742865592241287,
-0.004264530725777149,
-0.03264281153678894,
0.012454206123948097,
-0.009007603861391544,
-0.032502658665180206,
0.025881752371788025,
0.00609314301982522,
-0.01086270622909069,
0.03873581811785698,
0.06396271288394928,
-0.014133562333881855,
0.02019384130835533,
-0.04287808761000633,
-0.07056192308664322,
-0.06811241060495377,
0.02358563244342804,
0.06121644750237465,
-0.03550422191619873,
0.05408928543329239,
-0.009410184808075428,
-0.02542547695338726,
-0.030706314370036125,
-0.006958752870559692,
0.03651341050863266,
0.03627505525946617,
-0.036598268896341324,
0.07548583298921585,
0.045270372182130814,
-0.020751377567648888,
0.014633226208388805,
-0.01234179176390171,
-0.033159639686346054,
0.004913077224045992,
-0.011997551657259464,
-0.036397967487573624,
0.008026659488677979,
0.0034036466386169195,
0.02685018628835678,
-0.0026188124902546406,
-0.0487801730632782,
0.03394503518939018,
-0.06698133051395416,
-0.06316138058900833,
-0.03196166828274727,
0.11137094348669052,
-0.06760450452566147,
0.09097428619861603,
0.002810558071359992,
-0.05305924639105797,
-0.02113787643611431,
0.052100252360105515,
-0.015021247789263725,
0.03772520646452904,
-0.029368866235017776,
0.02526096999645233,
0.019866010174155235,
-0.002906883368268609,
0.03997277840971947,
0.004888337571173906,
-0.14480279386043549,
0.009010742418467999,
0.011788619682192802,
0.005470501258969307,
0.020088408142328262,
-0.00324986781924963,
-0.03435654938220978,
-0.03966439515352249,
-0.008058927021920681,
-0.022852612659335136,
-0.039991702884435654,
0.017588693648576736,
-0.022764066234230995,
-0.05008536949753761,
-0.037490468472242355,
0.050003595650196075,
0.016443409025669098,
0.05125333368778229,
-0.001964644994586706,
-0.011752612888813019,
-0.0069687641225755215,
-0.047297023236751556,
-0.029791129752993584,
0.01617884449660778,
0.035198505967855453,
-0.04829630255699158,
0.016422709450125694,
0.03253994509577751,
-0.0008385204127989709,
0.05204270780086517,
0.0041360934264957905,
-0.01361825130879879,
-0.06353078037500381,
0.010304954834282398,
0.014462929219007492,
-0.054409775882959366,
-0.022526618093252182,
0.021871542558073997,
0.030839046463370323,
0.01879769377410412,
0.07460471987724304,
0.0579884871840477,
0.003890282241627574,
-0.03236153721809387,
-0.0464547760784626,
0.034215569496154785,
-0.013421278446912766,
0.03925339877605438,
0.013616487383842468,
0.048087019473314285,
-0.06691127270460129,
-0.005263117607682943,
-0.11848823726177216,
-0.00959967914968729,
-0.08550390601158142,
0.009837512858211994,
-0.04141143336892128,
-0.03327310457825661,
0.06015680357813835,
-0.0038273485843092203,
0.01879081502556801,
0.038510434329509735,
-0.06122433394193649,
-0.2934911847114563,
-0.01681601256132126,
-0.0037272716872394085,
0.001276981201954186,
0.04775216802954674,
-0.02252485603094101,
-0.020753903314471245,
-0.015307403169572353,
-0.04095616191625595,
-0.05373041704297066,
0.069689080119133,
0.07507087290287018,
0.030977101996541023,
0.04170220345258713,
0.0011562301078811288,
-0.0003046262718271464,
0.018926456570625305,
-0.05361507833003998,
0.003835730254650116,
-0.009573730640113354,
0.029202736914157867,
-0.004832678474485874,
-0.003001300385221839,
0.0038727165665477514,
0.05572974681854248,
-0.014599787071347237,
0.053950242698192596,
-0.0027999470476061106,
0.035358332097530365,
0.005872188601642847,
-0.0024358173832297325,
0.07382926344871521,
-0.020014243200421333,
-0.10990209877490997,
-0.007922970689833164,
-0.00469377264380455,
0.0013906823005527258,
-0.04691609740257263,
-0.05720086023211479,
-0.019928433001041412,
0.009344663470983505,
-0.02234928123652935,
-0.06055174395442009,
-0.05665287375450134,
0.0014013798208907247,
-0.01591542176902294,
-0.010235496796667576,
0.10755428671836853,
0.012180224992334843,
0.01906401664018631,
0.023082442581653595,
-0.0077840425074100494,
0.02483980730175972,
-0.07135500013828278,
-0.03636578097939491,
0.015689151361584663,
0.00349497701972723,
0.015380406752228737,
-0.029318761080503464,
-0.000311225769110024,
-0.0603080578148365,
0.013848984614014626,
0.01841597445309162,
0.07679438591003418,
-0.005965355783700943,
-0.06334707140922546,
-0.009252256713807583,
0.011448251083493233,
0.01242700032889843,
0.02265881933271885,
-0.021459029987454414,
0.07524984329938889,
-0.03479861840605736,
0.0010530358413234353,
0.006737094838172197,
0.044121190905570984,
-0.018576307222247124,
-0.045308295637369156,
-0.05645325407385826,
-0.03464626893401146,
-0.0175219364464283,
0.005343994125723839,
0.08436314016580582,
-0.02041742578148842,
-0.022140564396977425,
0.04799903556704521,
0.029239989817142487,
0.017279494553804398,
0.03089033253490925,
0.020616214722394943,
-0.06719925999641418,
0.012728873640298843,
-0.04287524148821831,
-0.026386816054582596,
0.04670935124158859,
0.019895145669579506,
-0.2594723105430603,
-0.02625327743589878,
0.03387206420302391,
0.011401578783988953,
-0.01811232604086399,
0.05919899046421051,
0.05807157978415489,
-0.04147472605109215,
-0.017854591831564903,
0.0744895413517952,
0.016688888892531395,
0.03727798908948898,
0.07290779799222946,
0.05616946518421173,
-0.00668373191729188,
0.026065519079566002,
0.021168315783143044,
-0.0034847897477447987,
0.06893299520015717,
-0.04058615490794182,
0.00598627794533968,
-0.027097368612885475,
0.16686469316482544,
-0.03398376703262329,
0.06644139438867569,
0.03603864088654518,
-0.013331147842109203,
0.043282825499773026,
-0.04160130396485329,
-0.009887308813631535,
0.0440661646425724,
0.034817736595869064,
0.08650273084640503,
-0.07393696904182434,
0.014933109283447266,
0.04180029779672623,
-0.029995147138834,
-0.010915589518845081,
-0.051315635442733765,
0.02557367831468582,
0.03317711129784584,
-0.0147713553160429,
-0.07582852244377136,
-0.0029268611688166857,
0.05939563363790512,
-0.07517212629318237,
-0.02969256229698658,
0.0024224312510341406,
0.00848075095564127,
0.01868843473494053,
-0.04353620484471321,
0.011702097952365875,
0.03856881707906723,
0.03820202499628067,
-0.04098502919077873,
-0.016575532034039497,
-0.04183245822787285,
0.00305070704780519,
-0.01301596499979496,
-0.053116828203201294,
0.06357619166374207,
0.028761381283402443,
-0.011851131916046143,
0.08610428869724274,
0.0589032806456089
] |
PMID:25130 | Proteolytic activity of Trichoderma viride in mixed culture with Sclerotium rolfsii in soil. | Cultures of Sclerotium rolfsii and Trichoderma viride together in autoclaved soil were assayed at intervals during 8 days of incubation for proteolytic activity (PA) of T. viride. Significant proteolytic activity was detected only in soil containing T. viride (i.e., T. viride alone or S. rolfsii + T. viride); greatest activity occurred between 3 and 4 days after infestation and declined rapidly thereafter. Maximal PA in the mixed-culture soil was accompanied by an increase in soil pH. optimal pH values for PA was 5.5-6.5 with a maximum at 6.0. | Proteolytic activity of Trichoderma viride in mixed culture with Sclerotium rolfsii in soil. Cultures of Sclerotium rolfsii and Trichoderma viride together in autoclaved soil were assayed at intervals during 8 days of incubation for proteolytic activity (PA) of T. viride. Significant proteolytic activity was detected only in soil containing T. viride (i.e., T. viride alone or S. rolfsii + T. viride); greatest activity occurred between 3 and 4 days after infestation and declined rapidly thereafter. Maximal PA in the mixed-culture soil was accompanied by an increase in soil pH. optimal pH values for PA was 5.5-6.5 with a maximum at 6.0. | [
0.009002337232232094,
-0.008434317074716091,
-0.0010711877839639783,
0.03432777523994446,
0.053387850522994995,
-0.04591592028737068,
0.03615862876176834,
0.020385170355439186,
-0.0686241015791893,
0.008774299174547195,
0.03577970713376999,
-0.0805114358663559,
0.03526328131556511,
0.018047068268060684,
-0.05397871881723404,
-0.010517052374780178,
-0.0037852886598557234,
-0.011834759265184402,
0.028547359630465508,
-0.01666790433228016,
0.029730897396802902,
0.030156321823596954,
0.007882696576416492,
-0.042948972433805466,
0.03653287887573242,
0.07389503717422485,
-0.04079224169254303,
-0.022270498797297478,
0.0010186396539211273,
-0.18113374710083008,
0.06482288241386414,
-0.025400009006261826,
0.0010549386497586966,
-0.020892873406410217,
-0.0044759600423276424,
-0.009914757683873177,
-0.05085036903619766,
0.017535749822854996,
-0.04818786680698395,
0.0170676838606596,
0.03833071142435074,
0.07843660563230515,
-0.025455065071582794,
0.011521901935338974,
-0.005812282674014568,
-0.03282611817121506,
-0.0024352476466447115,
0.02130257524549961,
0.07655982673168182,
0.02881821244955063,
-0.019259029999375343,
-0.07500895112752914,
0.02026590332388878,
0.0014564594021067023,
-0.0071628098376095295,
-0.031169801950454712,
0.006430207286030054,
0.06499849259853363,
-0.021308209747076035,
0.01868617907166481,
0.03533298149704933,
0.05104091763496399,
-0.09855563938617706,
0.025922322645783424,
0.014503907412290573,
-0.030269263312220573,
-0.046573709696531296,
0.004797524306923151,
0.030917782336473465,
0.04629167169332504,
-0.024070698767900467,
0.08319955319166183,
0.029946940019726753,
0.030462153255939484,
0.006673702970147133,
0.043855808675289154,
-0.004320184700191021,
0.01866159215569496,
0.03669247776269913,
-0.035176217555999756,
-0.051771387457847595,
-0.006744937039911747,
0.02487681806087494,
0.03254295885562897,
-0.009960542432963848,
-0.0006258229841478169,
-0.0019168640719726682,
0.037482358515262604,
0.05098268389701843,
0.033249419182538986,
0.03183156996965408,
-0.02790204994380474,
-0.025303931906819344,
-0.020926546305418015,
-0.10057508200407028,
0.005459190811961889,
0.0045500462874770164,
-0.01595446653664112,
0.0036423183046281338,
0.40788036584854126,
-0.058380864560604095,
-0.040521830320358276,
-0.07299299538135529,
-0.004051016643643379,
-0.021208684891462326,
-0.02497675083577633,
-0.049279674887657166,
-0.025747042149305344,
-0.009760468266904354,
0.06593268364667892,
0.07907479256391525,
-0.004781221505254507,
-0.02516229823231697,
-0.042830128222703934,
0.015413624234497547,
-0.003350246464833617,
0.06619400531053543,
0.046308618038892746,
-0.018647372722625732,
0.03894614055752754,
-0.0025391841772943735,
0.04128781333565712,
0.03542974591255188,
-0.01880032569169998,
0.03315886855125427,
0.03761189058423042,
0.06612428277730942,
0.10016155242919922,
0.013231460005044937,
0.012052839621901512,
0.009119783528149128,
-0.014979776926338673,
-0.026478582993149757,
-0.009484314359724522,
-0.0032402679789811373,
-0.01716132089495659,
0.030555984005331993,
-0.01424997579306364,
0.04392995685338974,
-0.03479200601577759,
-0.06438279151916504,
-0.09615803509950638,
-0.08742889016866684,
-0.05236707627773285,
-0.05181021988391876,
0.04640395939350128,
0.01601436547935009,
0.050451941788196564,
0.047560010105371475,
0.0220168586820364,
-0.026118682697415352,
0.08985603600740433,
0.012606208212673664,
0.045198600739240646,
0.04549344629049301,
0.001410340191796422,
0.017508331686258316,
-0.07262837886810303,
0.018893195316195488,
-0.0476236566901207,
-0.12103396654129028,
-0.0038802579510957003,
0.030498437583446503,
0.09413686394691467,
0.0603121779859066,
-0.009384877048432827,
0.012845109216868877,
-0.021207323297858238,
0.01092227827757597,
-0.08838668465614319,
-0.018206574022769928,
0.03034314513206482,
-0.019395718351006508,
-0.042497146874666214,
-0.053459230810403824,
0.03046855889260769,
-0.07346400618553162,
0.0015222982037812471,
0.010514472611248493,
0.009865294210612774,
0.012715347111225128,
0.043861232697963715,
-0.03393503651022911,
0.04111340641975403,
0.019282085821032524,
0.009754400700330734,
-0.02267160266637802,
0.011384330689907074,
-0.03834814205765724,
0.0044675786048173904,
-0.03814198076725006,
-0.02828371152281761,
-0.059067897498607635,
0.015359983779489994,
-0.0012582605704665184,
-0.034612007439136505,
-0.05148949474096298,
-0.013797715306282043,
0.002527260221540928,
-0.01673290506005287,
0.10420551151037216,
0.048677124083042145,
0.014722438529133797,
0.00032726777135394514,
-0.008412743918597698,
0.06344003230333328,
-0.030970893800258636,
0.01896212436258793,
0.007432879414409399,
-0.003918041475117207,
-0.023668836802244186,
-0.0005218132864683867,
-0.016507389023900032,
0.029373912140727043,
-0.03650538623332977,
0.07561934739351273,
-0.007775839418172836,
-0.010726282373070717,
0.02161574549973011,
0.012283883057534695,
0.016264811158180237,
-0.016828548163175583,
-0.0418517179787159,
-0.3013083040714264,
-0.04394661635160446,
0.016033900901675224,
-0.01563609018921852,
0.05276650935411453,
-0.02015688084065914,
-0.02568862773478031,
-0.07352179288864136,
0.024369409307837486,
-0.09623323380947113,
0.05500778183341026,
0.07092707604169846,
0.02200240269303322,
-0.02472803369164467,
0.06013102829456329,
0.04967831075191498,
0.04862868785858154,
-0.030698612332344055,
-0.002700074343010783,
-0.007448195479810238,
0.0288709569722414,
-0.06857850402593613,
0.012243152596056461,
-0.01331629790365696,
0.04722725972533226,
-0.06798552721738815,
0.06780573725700378,
0.0030015073716640472,
0.0066698649898171425,
-0.02947554737329483,
0.013203952461481094,
0.037260960787534714,
-0.025316720828413963,
-0.0944051519036293,
-0.04952346906065941,
-0.052792325615882874,
0.042375627905130386,
-0.06097591295838356,
0.01281736884266138,
0.01523085217922926,
-0.0036351450253278017,
0.05415128916501999,
-0.030764201655983925,
-0.089621402323246,
0.0012020805152133107,
-0.03779918700456619,
0.015606779605150223,
0.018584609031677246,
0.059695664793252945,
-0.011820310726761818,
-0.018869729712605476,
0.03281048312783241,
0.053980693221092224,
0.018144622445106506,
-0.0037775076925754547,
0.024644261226058006,
-0.06724058836698532,
0.0645170733332634,
-0.04125787690281868,
-0.000552257290109992,
-0.01831662654876709,
0.016473807394504547,
-0.0035712725948542356,
0.040621303021907806,
0.046653568744659424,
0.005043131299316883,
-0.022939329966902733,
-0.062430016696453094,
0.05644618347287178,
0.020911863073706627,
-0.01011755783110857,
0.028002554550766945,
-0.03278825059533119,
0.02681373804807663,
-0.008673634380102158,
-0.009283804334700108,
-0.025271743535995483,
-0.03448573872447014,
-0.004344383254647255,
0.010355954989790916,
-0.0007430260884575546,
-0.04258420690894127,
0.013344327919185162,
-0.02218794636428356,
0.045319411903619766,
-0.008777630515396595,
-0.027720598503947258,
-0.03408171981573105,
0.021584728732705116,
-0.017717575654387474,
0.03822329640388489,
-0.03595473989844322,
-0.006139090750366449,
0.01442321203649044,
0.030654409900307655,
-0.010713783092796803,
-0.29840508103370667,
-0.03163107857108116,
0.07603229582309723,
-0.008558636531233788,
-0.003414130536839366,
0.04974479600787163,
0.023795781657099724,
0.004025785718113184,
0.01606437750160694,
-0.013863432221114635,
0.05602716654539108,
-0.006979573052376509,
0.040109291672706604,
0.019874371588230133,
0.06078806519508362,
0.05268894508481026,
0.0033893364015966654,
-0.03583664074540138,
0.03560732305049896,
-0.08802662789821625,
0.015182485803961754,
-0.06650872528553009,
0.1491394340991974,
0.05182886868715286,
-0.012535692192614079,
0.032817292958498,
0.022824088111519814,
0.006704261992126703,
-0.018529638648033142,
0.006154677830636501,
0.0017380901845172048,
-0.013892036862671375,
0.08314050734043121,
0.002435514936223626,
-0.014540567062795162,
0.01950751431286335,
0.011405566707253456,
-0.02672349102795124,
-0.06688293069601059,
0.06290530413389206,
-0.015739569440484047,
-0.01984182558953762,
-0.08344722539186478,
-0.02238202653825283,
0.020677689462900162,
-0.09760650992393494,
-0.028089428320527077,
-0.05855263024568558,
-0.02611721307039261,
0.0029897962231189013,
-0.03247398883104324,
-0.05695780739188194,
0.020460931584239006,
0.043296441435813904,
-0.020119862630963326,
0.04546765983104706,
0.003152933670207858,
0.007614687085151672,
-0.035350002348423004,
0.04904419556260109,
0.06373891234397888,
-0.02717631496489048,
-0.028445176780223846,
0.045715056359767914,
0.024982646107673645
] |
PMID:25135 | Proliferation and viability in cellular spheroids of human origin. | The capacity of cells to form growing tumor-like colonies in culture was tested by a new and unlaborious suspension technique. Six of nine tested cell lines formed spheroids, five of which started to grow. The growing spheroids reached a maximal size determined by balanced cell gain in a proliferative, superficial layer and cell death, particularly at larger depth. Sections of spheroids of different origin showed large variations in the thickness of the viable cell layers and in the shape of the proliferative gradients. The data, taken together with earlier published information, indicate that a difference between rodent and human cells generally exists, the former showing thinner viable and proliferative layers. | Proliferation and viability in cellular spheroids of human origin. The capacity of cells to form growing tumor-like colonies in culture was tested by a new and unlaborious suspension technique. Six of nine tested cell lines formed spheroids, five of which started to grow. The growing spheroids reached a maximal size determined by balanced cell gain in a proliferative, superficial layer and cell death, particularly at larger depth. Sections of spheroids of different origin showed large variations in the thickness of the viable cell layers and in the shape of the proliferative gradients. The data, taken together with earlier published information, indicate that a difference between rodent and human cells generally exists, the former showing thinner viable and proliferative layers. | [
-0.04237457737326622,
-0.0340813584625721,
0.02050052024424076,
-0.00456690788269043,
-0.01670837588608265,
0.07853130996227264,
0.04628566652536392,
0.028619999065995216,
0.008628768846392632,
0.0012508364161476493,
0.03233759105205536,
-0.06502904742956161,
0.012404258362948895,
0.019549107179045677,
-0.05282639339566231,
-0.014611653052270412,
-0.04425014555454254,
-0.009510308504104614,
-0.0582839772105217,
0.014461318030953407,
-0.008830069564282894,
-0.06378784030675888,
-0.01578880473971367,
-0.038839876651763916,
0.03022323176264763,
-0.007178330793976784,
-0.036362022161483765,
0.016801785677671432,
-0.02026802860200405,
-0.1800803691148758,
0.018543457612395287,
-0.030047766864299774,
-0.007442997302860022,
0.006921989843249321,
-0.03357964754104614,
-0.023471329361200333,
0.011744149960577488,
-0.052833981812000275,
0.020716723054647446,
0.041370607912540436,
-0.014674331992864609,
0.004066334571689367,
0.010447134263813496,
0.03570421040058136,
0.014791985042393208,
0.07619652152061462,
-0.019523054361343384,
-0.023354727774858475,
0.025170888751745224,
0.013696440495550632,
0.021877769380807877,
-0.0408153161406517,
0.010221501812338829,
0.037917573004961014,
-0.02750723995268345,
-0.03317014500498772,
0.009984101168811321,
0.047117602080106735,
0.056343697011470795,
0.04015827924013138,
-0.0182302575558424,
0.0015841175336390734,
-0.11442117393016815,
0.06071951612830162,
0.0489036999642849,
-0.030174128711223602,
-0.04845913127064705,
-0.04327895864844322,
0.02159513160586357,
0.0413539744913578,
-0.022126486524939537,
0.03842515870928764,
0.07417116314172745,
0.048656560480594635,
-0.01679585874080658,
0.025144729763269424,
0.0004911451833322644,
-0.035997211933135986,
0.03614584729075432,
0.008833161555230618,
-0.01015505287796259,
0.006495199166238308,
0.01480130571871996,
-0.007051549386233091,
-0.07108306884765625,
-0.013701152987778187,
-0.049823008477687836,
-0.022205308079719543,
-0.0318719856441021,
0.04897712543606758,
-0.003679693676531315,
0.04697339981794357,
-0.013892192393541336,
0.02857581339776516,
-0.07275830954313278,
-0.055500879883766174,
-0.005195854231715202,
-0.04669441655278206,
0.07058192044496536,
0.39160653948783875,
-0.017513642087578773,
-0.010656380094587803,
-0.032944485545158386,
0.06090869754552841,
-0.03209433704614639,
0.02420668676495552,
-0.054441142827272415,
-0.010698539204895496,
0.05789606273174286,
0.02674163319170475,
0.041516516357660294,
0.012022493407130241,
-0.039349764585494995,
0.0026003471575677395,
-0.02262711338698864,
-0.11789006739854813,
0.0375952385365963,
-0.05591427534818649,
-0.06798086315393448,
0.0018181317718699574,
0.039652418345212936,
-0.015384066849946976,
0.0126248924061656,
-0.040920440107584,
0.041357386857271194,
0.036703936755657196,
0.010567033663392067,
0.06071251258254051,
0.046389125287532806,
-0.0471632145345211,
0.050047390162944794,
0.059247974306344986,
-0.06007242575287819,
-0.030405467376112938,
0.04390638694167137,
-0.050995174795389175,
-0.00802587065845728,
-0.06639934331178665,
-0.03783702850341797,
-0.03298695385456085,
-0.0025407811626791954,
-0.02866506576538086,
0.005564318038523197,
-0.047553565353155136,
-0.03410713002085686,
0.10983715206384659,
0.008064446039497852,
0.0810326412320137,
-0.026405157521367073,
0.019007230177521706,
-0.013370614498853683,
0.017871826887130737,
0.014506039209663868,
-0.01627322845160961,
0.018807848915457726,
0.02333999238908291,
-0.029913518577814102,
-0.0015233412850648165,
-0.03298736736178398,
-0.02136288955807686,
-0.011628571897745132,
0.05806996673345566,
0.01201923843473196,
0.08279144018888474,
0.02536304108798504,
-0.06667070090770721,
-0.053853217512369156,
0.013774638995528221,
0.09033354371786118,
-0.01899867132306099,
0.023790575563907623,
-0.05479881912469864,
-0.020850753411650658,
0.011981511488556862,
-0.04881017655134201,
0.006784085184335709,
-0.07686197012662888,
-0.04308757930994034,
0.0887037143111229,
0.031187137588858604,
0.08674170821905136,
-0.020780198276042938,
0.027707604691386223,
0.015836235135793686,
-0.04430263862013817,
-0.02026878483593464,
-0.06073381006717682,
-0.009272683411836624,
0.03002540022134781,
0.049107350409030914,
-0.028787193819880486,
-0.044587552547454834,
-0.045451827347278595,
0.016213202849030495,
-0.04746510460972786,
-0.022531375288963318,
-0.026510827243328094,
-0.023364713415503502,
-0.003146630944684148,
-0.01761459745466709,
0.049392953515052795,
-0.037273578345775604,
0.013845967128872871,
0.005934619344770908,
0.01872989907860756,
0.07728774100542068,
0.09468135237693787,
0.04477653652429581,
0.09205352514982224,
0.027069643139839172,
-0.11120951175689697,
0.008822109550237656,
-0.0095668351277709,
-0.02141191065311432,
-0.0016176810022443533,
0.02422444522380829,
0.0024801772087812424,
-0.03539998456835747,
0.03988227993249893,
0.009165331721305847,
0.06578472256660461,
-0.07315556704998016,
-0.04787798225879669,
-0.29753032326698303,
-0.03485379368066788,
-0.014161961153149605,
-0.039740320295095444,
0.019374484196305275,
0.0006432224181480706,
0.04293770343065262,
-0.025950971990823746,
0.008684213273227215,
-0.077874094247818,
0.029738040640950203,
0.06971640884876251,
-0.02954697795212269,
-0.03905341401696205,
0.024484893307089806,
0.03595694527029991,
-0.012177633121609688,
-0.022381894290447235,
0.011994978412985802,
0.010494432412087917,
-0.03678023815155029,
-0.0006189587875269353,
-0.0028257740195840597,
0.006584566086530685,
0.049269381910562515,
-0.02013760805130005,
0.12422137707471848,
-0.014717931859195232,
-0.05587857961654663,
-0.011794693768024445,
0.016084592789411545,
0.011888675391674042,
0.0020894191693514585,
-0.039674725383520126,
0.008849726989865303,
0.0119599224999547,
-0.00011335714225424454,
-0.05710690841078758,
-0.01349620521068573,
0.020090559497475624,
0.0035702167078852654,
-0.021794864907860756,
-0.03755209594964981,
-0.0762355774641037,
-0.030963625758886337,
-0.03456711024045944,
0.019353199750185013,
0.04574170336127281,
0.005290897097438574,
-0.03496578335762024,
0.006691411603242159,
-0.026045121252536774,
-0.024592002853751183,
-0.00462711276486516,
0.016418687999248505,
-0.0004623675486072898,
-0.05755113810300827,
0.05632460117340088,
-0.04744308441877365,
0.04652142897248268,
-0.03892824053764343,
-0.0021314865443855524,
0.004408085718750954,
-0.00548715190961957,
0.00942100677639246,
0.008689270354807377,
-0.02109181135892868,
-0.015675073489546776,
0.04089532047510147,
-0.06806797534227371,
0.018857261165976524,
0.11412973701953888,
-0.007741823326796293,
0.05243324115872383,
0.052605003118515015,
-0.0027953169774264097,
-0.010445673018693924,
0.004243628587573767,
-0.0027533122338354588,
0.020808110013604164,
0.08672938495874405,
-0.04240117594599724,
0.058136168867349625,
0.021525736898183823,
0.04114917665719986,
0.08762602508068085,
0.012016930617392063,
-0.024530792608857155,
0.02184184268116951,
-0.030292054638266563,
0.022195961326360703,
0.01644119992852211,
-0.037749629467725754,
-0.07358616590499878,
-0.02188924327492714,
-0.05178900435566902,
-0.297599732875824,
0.029010802507400513,
0.03461360186338425,
0.080860435962677,
-0.04619278386235237,
-0.019494159147143364,
0.04022010788321495,
0.04313800856471062,
0.027762815356254578,
0.04760202765464783,
0.04069686308503151,
-0.03301325440406799,
0.03886515647172928,
0.021247131749987602,
0.008101055398583412,
0.027554204687476158,
0.02172143943607807,
-0.05357804149389267,
0.019923629239201546,
-0.048164308071136475,
0.04774823784828186,
-0.062056612223386765,
0.13903173804283142,
-0.04312606528401375,
-0.00842135027050972,
-0.003711933735758066,
0.03704322502017021,
0.04135644808411598,
-0.020443402230739594,
0.00047863859799690545,
-0.0229899100959301,
0.02626221254467964,
0.01715298928320408,
-0.018119778484106064,
-0.008498173207044601,
0.06599289178848267,
0.03929250314831734,
0.005429060198366642,
-0.005723089911043644,
0.007978800684213638,
0.01476135104894638,
-0.00564373005181551,
-0.06615309417247772,
0.08566272258758545,
0.059932541102170944,
-0.023284830152988434,
0.03810334578156471,
-0.02731211669743061,
0.026560917496681213,
0.0071173314936459064,
-0.08385720103979111,
-0.028363248333334923,
0.012003329582512379,
-0.009613466449081898,
-0.008512087166309357,
0.018690887838602066,
0.043689291924238205,
-0.02889985404908657,
0.019168328493833542,
0.04071148857474327,
0.04437815397977829,
-0.00004704026287072338,
-0.008494662120938301,
0.10174965113401413,
-0.036623697727918625
] |
PMID:25144 | Enzymatic determination of blood ethanol, with amperometric measurement of rate of oxygen depletion. | A rapid electrochemical measurement of blood ethanol is proposed. Alcohol is oxidized by NAD+ in the presence of alcohol dehydrogenase; and the NADH produced is aerobically oxidized by horseradish peroxidase. The rate of depletion of buffer-carried oxygen, which is directly proportional to the alcohol concentration in the sample, is amperometrically monitored with a membrane oxygen-sensing electrode. Only a 5-microliter sample of whole blood is required, with no deproteinization, incubation, extraction, or dilution. Results, obtained in less than 1 min, correlate well with those obtained by gas-chromatographic and spectrophotometric methods. | Enzymatic determination of blood ethanol, with amperometric measurement of rate of oxygen depletion. A rapid electrochemical measurement of blood ethanol is proposed. Alcohol is oxidized by NAD+ in the presence of alcohol dehydrogenase; and the NADH produced is aerobically oxidized by horseradish peroxidase. The rate of depletion of buffer-carried oxygen, which is directly proportional to the alcohol concentration in the sample, is amperometrically monitored with a membrane oxygen-sensing electrode. Only a 5-microliter sample of whole blood is required, with no deproteinization, incubation, extraction, or dilution. Results, obtained in less than 1 min, correlate well with those obtained by gas-chromatographic and spectrophotometric methods. | [
-0.03322320058941841,
0.02762056700885296,
-0.019624998793005943,
-0.0002562291920185089,
0.02614748105406761,
-0.04321800172328949,
0.05662095919251442,
0.08653848618268967,
-0.025302307680249214,
-0.027111707255244255,
0.014744900166988373,
-0.023829972371459007,
-0.06047261133790016,
0.019319970160722733,
-0.05019798129796982,
-0.03938654065132141,
0.05235698074102402,
-0.012010821141302586,
-0.05070590227842331,
-0.00929009635001421,
0.0012032449012622237,
-0.02546224556863308,
-0.019442684948444366,
0.017973963171243668,
0.06190996989607811,
-0.01001095212996006,
0.00957145169377327,
0.0029539295937865973,
-0.10270413011312485,
-0.13371285796165466,
0.03694828599691391,
-0.008947934955358505,
-0.005474764853715897,
-0.019771017134189606,
-0.004844695329666138,
0.020062675699591637,
0.00009252983727492392,
-0.015892788767814636,
0.002219840884208679,
0.020174654200673103,
0.024217136204242706,
-0.022043514996767044,
-0.03337433561682701,
0.02282213233411312,
0.019861632958054543,
0.014248076826334,
-0.007792633026838303,
0.0345357209444046,
0.056940507143735886,
-0.04864462837576866,
0.05888471379876137,
0.006355701945722103,
0.0726897120475769,
-0.039155203849077225,
0.04898274317383766,
-0.0808207169175148,
-0.00003409259443287738,
0.01080442313104868,
-0.02973414771258831,
-0.0234368983656168,
-0.015795109793543816,
0.006144190672785044,
-0.1369248628616333,
0.014793192967772484,
0.022566664963960648,
-0.04755792394280434,
-0.028862565755844116,
-0.06515946984291077,
0.013372995890676975,
0.026567092165350914,
-0.05111503601074219,
0.03774968907237053,
0.0018398207612335682,
0.012726839631795883,
-0.07672648876905441,
-0.04065568372607231,
0.03177331015467644,
-0.059303030371665955,
0.021754443645477295,
0.02024114690721035,
0.04316655546426773,
-0.009715575724840164,
0.027138561010360718,
-0.005150168668478727,
-0.018771808594465256,
0.02800440415740013,
0.02918178401887417,
-0.03499233350157738,
0.0005674424464814365,
0.0408858023583889,
-0.03693357855081558,
-0.09177631139755249,
-0.009397340007126331,
0.00685700960457325,
-0.06347443908452988,
-0.006033163517713547,
0.012811482883989811,
0.0009026294574141502,
0.0039797392673790455,
0.37594959139823914,
0.01037664245814085,
0.008139073848724365,
-0.0001372926344629377,
-0.08064961433410645,
-0.014453067444264889,
-0.06611305475234985,
-0.0337994247674942,
0.03038584068417549,
-0.033162154257297516,
0.0589916817843914,
0.012518048286437988,
-0.013155009597539902,
0.03748707100749016,
-0.04818841069936752,
-0.033248286694288254,
0.07593081891536713,
-0.016634108498692513,
0.0005546333268284798,
-0.01384003460407257,
0.05010172724723816,
-0.015812695026397705,
0.004088884685188532,
0.056684400886297226,
-0.007779229432344437,
0.10302110016345978,
-0.010003386065363884,
-0.0012378693791106343,
0.09233065694570541,
0.039904650300741196,
0.03594934940338135,
0.04940614104270935,
-0.0070185852237045765,
-0.0071768993511796,
-0.02655864879488945,
-0.08102691918611526,
0.012646946124732494,
0.03206168860197067,
0.016986416652798653,
0.04191742464900017,
-0.007562313228845596,
-0.054170556366443634,
0.008652741089463234,
0.019826101139187813,
-0.03958455100655556,
-0.05676447972655296,
0.038302574306726456,
0.0618806853890419,
0.0021881654392927885,
-0.016640815883874893,
-0.040553487837314606,
-0.010489786975085735,
0.030515599995851517,
-0.05698499083518982,
0.02293650060892105,
0.002810759237036109,
-0.0020266217179596424,
0.00223125284537673,
-0.0007081556832417846,
0.021592099219560623,
-0.0010505585232749581,
-0.04810785502195358,
0.024367274716496468,
0.02679128758609295,
0.06558069586753845,
0.007925357669591904,
-0.020943408831954002,
-0.034576527774333954,
0.027847779914736748,
0.012986957095563412,
0.004887068178504705,
0.033099427819252014,
-0.024361100047826767,
0.0033165132626891136,
-0.007196894381195307,
0.01472117193043232,
0.009900725446641445,
0.049394670873880386,
0.10432423651218414,
0.034244220703840256,
0.028550192713737488,
0.0716434195637703,
0.010393652133643627,
-0.03544651344418526,
0.0941029042005539,
0.03946968540549278,
0.007705158554017544,
-0.03339914605021477,
-0.04074133560061455,
0.02441384457051754,
0.08682318776845932,
-0.048095930367708206,
0.037941500544548035,
-0.07823554426431656,
-0.01185403298586607,
0.02768201194703579,
-0.015102589502930641,
-0.017055319622159004,
-0.044004470109939575,
-0.044865306466817856,
0.03282364830374718,
0.08064557611942291,
0.02694711461663246,
0.00812520831823349,
0.10544496029615402,
0.07056725770235062,
0.02796604484319687,
0.0022993916645646095,
0.004018327686935663,
0.008187864907085896,
0.055677447468042374,
-0.007668518926948309,
-0.031739506870508194,
-0.02234104834496975,
-0.04412075877189636,
-0.09555212408304214,
-0.002009457442909479,
-0.0056407456286251545,
-0.014145057648420334,
-0.0012434176169335842,
-0.002320033498108387,
0.020426498726010323,
-0.10005614161491394,
0.002220223192125559,
-0.32983750104904175,
-0.010164615698158741,
-0.010406596586108208,
0.04151216894388199,
0.047725360840559006,
-0.019650688394904137,
0.03043430857360363,
-0.06784093379974365,
-0.02766609378159046,
-0.04580524563789368,
0.06586863845586777,
0.03111148625612259,
-0.006853777449578047,
-0.033195432275533676,
0.007945769466459751,
0.06667456030845642,
0.029394136741757393,
-0.08853722363710403,
0.022729363292455673,
0.013458789326250553,
0.07357364892959595,
0.03387925401329994,
0.04858566075563431,
0.03614020720124245,
0.08887965232133865,
-0.08295218646526337,
0.1194327175617218,
-0.04064092040061951,
0.030094638466835022,
0.001323239179328084,
-0.04553454369306564,
-0.021496983245015144,
-0.011288845911622047,
-0.07195574045181274,
-0.03662984445691109,
-0.06865647435188293,
-0.018312843516469002,
0.011650963686406612,
-0.013092915527522564,
-0.044165339320898056,
-0.02300572209060192,
0.02219788171350956,
0.01981199160218239,
-0.07198382914066315,
-0.05682756006717682,
-0.019092708826065063,
-0.00021324462431948632,
-0.023322027176618576,
-0.0264543816447258,
0.005862859543412924,
0.07351364195346832,
-0.034677401185035706,
0.06944455951452255,
-0.048000503331422806,
0.039115820080041885,
-0.028106961399316788,
-0.03935958817601204,
0.032194312661886215,
-0.03372715413570404,
-0.037602901458740234,
0.009049005806446075,
-0.01545825693756342,
-0.020027831196784973,
0.020808489993214607,
0.011937184259295464,
0.02633458562195301,
0.02140960656106472,
-0.053473204374313354,
0.09600982815027237,
0.011743694543838501,
0.01565677672624588,
0.1295463591814041,
-0.02128850854933262,
-0.04761400446295738,
0.050810154527425766,
0.025920746847987175,
-0.005286145955324173,
-0.0570555180311203,
-0.040620025247335434,
0.04995501786470413,
0.00014873300096951425,
-0.04982886090874672,
0.07512664794921875,
-0.026002584025263786,
0.0023196269758045673,
0.06726528704166412,
0.02284289337694645,
-0.042507439851760864,
0.02235744334757328,
0.006198059767484665,
0.04035555198788643,
-0.0010867452947422862,
-0.009659450501203537,
-0.07732916623353958,
-0.016077682375907898,
0.0607902817428112,
-0.23549878597259521,
-0.04823746159672737,
-0.005621575750410557,
-0.01821295917034149,
0.01731366664171219,
0.024237114936113358,
0.01377059891819954,
-0.032328128814697266,
-0.019103912636637688,
-0.010195556096732616,
-0.05581694096326828,
0.003983843140304089,
-0.0019014851422980428,
-0.02080897055566311,
0.05621267482638359,
0.01963108777999878,
0.0358254574239254,
-0.055556315928697586,
0.01263903547078371,
-0.02723398432135582,
0.034097012132406235,
-0.0849175974726677,
0.09995729476213455,
-0.033281195908784866,
-0.054702114313840866,
0.0670916959643364,
0.0091741643846035,
0.010056985542178154,
0.012820765376091003,
-0.010849234648048878,
0.010290301404893398,
0.006109490059316158,
0.061401475220918655,
0.0051934258081018925,
0.06514003127813339,
0.013854548335075378,
-0.010256338864564896,
-0.009301197715103626,
0.026538072153925896,
0.006151540670543909,
0.0289897620677948,
-0.03864983841776848,
-0.07044456899166107,
-0.07842623442411423,
0.0651807188987732,
-0.015626607462763786,
-0.05300632119178772,
-0.08735030889511108,
-0.007713747676461935,
0.03574914485216141,
-0.05010778456926346,
0.02158215455710888,
0.0366828516125679,
0.09654649347066879,
0.07712377607822418,
-0.01842123083770275,
-0.030390826985239983,
-0.009416289627552032,
0.01938258297741413,
-0.003779354738071561,
-0.046874694526195526,
0.047620560973882675,
0.0029280283488333225,
0.08367127925157547,
0.008934910409152508
] |
PMID:25145 | Simultaneous liquid-chromatographic determination of 12 common sedatives and hypnotics in serum. | We present a method for simultaneously determining 12 hypnotics and sedatives (primidone, methyprylon, phenobarbital, butabarbital, butalbital, ethchlorvynol, pentobarbital, amobarbital, phenytoin, glutethimide, secobarbital and methaqualone) in 200 microliter of serum. Serum proteins are precipitated with an acetonitrile solution containing 5-(4-methylphenyl)-5-phenylhydantoin, the internal standard. The drugs are eluted from a reversed-phase column with a mobile phase consisting of an acetonitrile/phosphate buffer, at a flow rate of 3.0 ml/min. The eluted drugs are detected by their absorption at 195 nm; their quantities are estimated from their peak heights. Each analysis requires no longer than 30 min at the optimum column temperature of 50 degrees C. The lower limit of detection for all of these drugs is less than 10 ng/sample for drug standard. A sensitivity of 1.0 mg/liter of serum is attained routinely for each of the drugs. Analytical recoveries for the 12 drugs varied from 94 to 112%, with good day-to-day precision (CV between 3.8 and 10.4%). Of more than 35 drugs tested for possible interference, only ethotoin interferes with the analysis of phenobarbital. | Simultaneous liquid-chromatographic determination of 12 common sedatives and hypnotics in serum. We present a method for simultaneously determining 12 hypnotics and sedatives (primidone, methyprylon, phenobarbital, butabarbital, butalbital, ethchlorvynol, pentobarbital, amobarbital, phenytoin, glutethimide, secobarbital and methaqualone) in 200 microliter of serum. Serum proteins are precipitated with an acetonitrile solution containing 5-(4-methylphenyl)-5-phenylhydantoin, the internal standard. The drugs are eluted from a reversed-phase column with a mobile phase consisting of an acetonitrile/phosphate buffer, at a flow rate of 3.0 ml/min. The eluted drugs are detected by their absorption at 195 nm; their quantities are estimated from their peak heights. Each analysis requires no longer than 30 min at the optimum column temperature of 50 degrees C. The lower limit of detection for all of these drugs is less than 10 ng/sample for drug standard. A sensitivity of 1.0 mg/liter of serum is attained routinely for each of the drugs. Analytical recoveries for the 12 drugs varied from 94 to 112%, with good day-to-day precision (CV between 3.8 and 10.4%). Of more than 35 drugs tested for possible interference, only ethotoin interferes with the analysis of phenobarbital. | [
-0.050315599888563156,
0.032911140471696854,
0.046216174960136414,
0.006404630374163389,
0.005335475783795118,
-0.030437709763646126,
0.09094143658876419,
0.019362473860383034,
-0.02014590986073017,
-0.01050636637955904,
-0.04321987181901932,
-0.022480929270386696,
0.012971186079084873,
0.03575970605015755,
-0.022884268313646317,
-0.02457507699728012,
0.04682743549346924,
-0.03539023548364639,
-0.0014190759975463152,
0.032154034823179245,
-0.0021818613167852163,
-0.022066179662942886,
0.027004949748516083,
-0.011106607504189014,
0.032205164432525635,
0.04159197956323624,
-0.014936814084649086,
-0.015218300744891167,
-0.0203752089291811,
-0.16643746197223663,
0.06324085593223572,
0.008965439163148403,
0.009244951419532299,
-0.08136608451604843,
-0.0213557630777359,
0.008651933632791042,
-0.019687164574861526,
0.017525827512145042,
0.010463114827871323,
0.007046456914395094,
0.01096750982105732,
0.003970359452068806,
-0.052362918853759766,
0.02110651694238186,
0.03990752622485161,
-0.028426237404346466,
-0.07093485444784164,
0.035094745457172394,
0.10728896409273148,
0.001573388115502894,
0.029166359454393387,
0.007498808670789003,
0.06147239729762077,
0.07618247717618942,
-0.03138609603047371,
-0.018145084381103516,
-0.05966641753911972,
0.019558491185307503,
-0.041388098150491714,
0.08072205632925034,
-0.043557602912187576,
0.03696948662400246,
-0.12700603902339935,
0.033649202436208725,
0.012677326798439026,
-0.0033612565603107214,
-0.048329200595617294,
-0.031581148505210876,
0.030722297728061676,
0.012095717713236809,
0.0002974984818138182,
-0.008368859998881817,
-0.01093217171728611,
-0.000911108567379415,
-0.016026532277464867,
-0.053013961762189865,
0.05116641893982887,
-0.027720041573047638,
-0.027779387310147285,
-0.0032115639187395573,
0.01404124777764082,
-0.012446166016161442,
0.041113901883363724,
0.0040447586216032505,
0.08427887409925461,
-0.0020800805650651455,
0.05363575369119644,
0.07176047563552856,
0.08352874964475632,
-0.014713607728481293,
0.010339413769543171,
-0.058893196284770966,
-0.00782068818807602,
-0.002135939197614789,
0.009943806566298008,
-0.01843353733420372,
-0.04251336306333542,
-0.014656157232820988,
0.027297085151076317,
0.42747482657432556,
0.023395657539367676,
0.010779926553368568,
-0.07970156520605087,
-0.01149889174848795,
-0.007974814623594284,
-0.03637750819325447,
0.021362993866205215,
-0.03407954424619675,
0.01934286206960678,
-0.0012021451257169247,
-0.02309873141348362,
0.0032215153332799673,
0.1219303160905838,
-0.0523226261138916,
0.005082284566015005,
-0.025600451976060867,
0.02583271637558937,
-0.044268764555454254,
0.03258073329925537,
0.016012942418456078,
-0.00008971917850431055,
0.009846823289990425,
0.04508773982524872,
-0.011231055483222008,
0.034765563905239105,
-0.03910956531763077,
-0.022395553067326546,
0.08084232360124588,
0.019262190908193588,
0.016130480915308,
0.008316166698932648,
0.025739671662449837,
0.012725993990898132,
0.025238923728466034,
0.005899867508560419,
0.00760727608576417,
0.02431461773812771,
-0.020772181451320648,
0.11492408812046051,
-0.023883240297436714,
-0.023044582456350327,
-0.04346877336502075,
-0.0046343314461410046,
-0.0661231130361557,
-0.04763791337609291,
0.09716971218585968,
-0.0023127750027924776,
-0.027327388525009155,
-0.01894262619316578,
-0.05509877949953079,
-0.031224971637129784,
0.004919068887829781,
-0.03788827732205391,
0.017851488664746284,
-0.0034458155278116465,
0.027190623804926872,
0.07134547829627991,
-0.02975608967244625,
-0.04875790327787399,
0.01863809861242771,
-0.006289380602538586,
-0.012312891893088818,
-0.008744949474930763,
0.094988614320755,
-0.05792117491364479,
-0.031969115138053894,
0.040354132652282715,
-0.016841493546962738,
-0.03373768925666809,
-0.016396161168813705,
0.020768191665410995,
0.021189989522099495,
-0.011416456662118435,
0.042573802173137665,
-0.07797472178936005,
-0.04950437322258949,
0.051896534860134125,
0.032486964017152786,
-0.007823064923286438,
0.0027426169253885746,
0.004923530388623476,
0.044756606221199036,
-0.0889471024274826,
0.025363823398947716,
-0.012226716615259647,
-0.0073531013913452625,
-0.006716123316437006,
0.016976546496152878,
-0.04805140197277069,
0.04984952509403229,
-0.016661880537867546,
0.04971647262573242,
-0.056951288133859634,
0.029701605439186096,
-0.05244867131114006,
-0.026511451229453087,
-0.04252788424491882,
0.006340087857097387,
-0.01683923229575157,
-0.02416931837797165,
0.11459551751613617,
0.04886415973305702,
0.04299155995249748,
0.05089044198393822,
0.10620801150798798,
0.04859582707285881,
-0.02660198137164116,
0.0037296265363693237,
0.05228303745388985,
-0.018438395112752914,
0.000692513887770474,
0.024576321244239807,
-0.0155820706859231,
0.027300085872411728,
-0.05057324469089508,
0.012037091888487339,
-0.003268019761890173,
-0.012710283510386944,
0.039037272334098816,
0.0047746943309903145,
0.016440069302916527,
-0.029831821098923683,
-0.005480168387293816,
-0.3175930380821228,
-0.004229546990245581,
0.03857460245490074,
-0.0004879680054727942,
0.03739411011338234,
-0.01988707296550274,
-0.0008500706171616912,
-0.01732407510280609,
-0.0023672515526413918,
-0.012434061616659164,
0.06286744028329849,
0.0615215077996254,
-0.06795266270637512,
-0.011615513823926449,
-0.035726580768823624,
0.034180186688899994,
0.004683957900851965,
0.03095078468322754,
0.0118886549025774,
0.0005261587211862206,
-0.011094747111201286,
-0.0182326827198267,
0.0072778440080583096,
0.052720170468091965,
0.05525002256035805,
-0.018650883808732033,
0.15226541459560394,
0.009687314741313457,
-0.05096906051039696,
-0.030952855944633484,
-0.025911156088113785,
0.008456685580313206,
0.004789423663169146,
-0.10018759220838547,
0.012715497054159641,
-0.04669950529932976,
0.006593500729650259,
0.007336722686886787,
0.005332531873136759,
-0.04414304345846176,
-0.05905744433403015,
0.02666435018181801,
0.04875461012125015,
-0.03417956456542015,
-0.09598545730113983,
-0.008849564008414745,
-0.02843765914440155,
-0.03566766902804375,
0.001729096402414143,
0.028628872707486153,
0.019303910434246063,
0.005496525671333075,
0.04247026517987251,
-0.02678253874182701,
0.010287268087267876,
-0.023544559255242348,
-0.04924945533275604,
-0.028676318004727364,
-0.06648990511894226,
-0.026903901249170303,
0.029656225815415382,
-0.029747245833277702,
0.018300212919712067,
-0.005732591729611158,
0.012843497097492218,
-0.009938118979334831,
-0.015118617564439774,
-0.0348271019756794,
0.10726994276046753,
0.03813643008470535,
-0.017743708565831184,
0.11893706023693085,
-0.05797210708260536,
-0.02174244076013565,
0.03427121415734291,
0.011304396204650402,
0.009670292027294636,
-0.05216377601027489,
-0.08514171838760376,
0.037976931780576706,
-0.021271197125315666,
-0.013558306731283665,
-0.03132854029536247,
-0.0018977407598868012,
-0.014666717499494553,
0.002349413000047207,
0.023718470707535744,
0.024872584268450737,
0.04407759755849838,
0.007238736841827631,
-0.0017382772639393806,
0.031739432364702225,
0.01891099102795124,
-0.036318160593509674,
-0.0018759171944111586,
0.07626444846391678,
-0.28741493821144104,
-0.012031064368784428,
-0.029069192707538605,
-0.01511247456073761,
0.024601643905043602,
0.002516006352379918,
0.047788459807634354,
-0.03823193535208702,
-0.05292493849992752,
-0.02469831332564354,
-0.021267255768179893,
0.03770788386464119,
0.041074417531490326,
-0.006259073968976736,
-0.0461609810590744,
-0.00573734100908041,
0.04177618771791458,
-0.05206308513879776,
0.027195818722248077,
-0.013061645440757275,
0.024946419522166252,
0.007200860884040594,
0.09412147849798203,
-0.0014262397307902575,
-0.03361968696117401,
0.037432748824357986,
0.08129788935184479,
0.028398403897881508,
-0.013725190423429012,
0.052084535360336304,
-0.012679453007876873,
0.028667345643043518,
0.02384299412369728,
-0.04244128242135048,
-0.0008248449303209782,
0.02954154834151268,
-0.07142617553472519,
-0.02327531762421131,
0.019999174401164055,
0.016758080571889877,
0.019286813214421272,
-0.004140527918934822,
-0.02687477134168148,
-0.019797731190919876,
0.041322603821754456,
-0.06810891628265381,
-0.0706329420208931,
-0.03658217936754227,
0.014143900014460087,
0.02733801119029522,
-0.026339897885918617,
0.021324971690773964,
-0.013409660197794437,
0.04832719266414642,
0.026950476691126823,
0.011042366735637188,
-0.029003066942095757,
-0.01700553670525551,
0.008738964796066284,
-0.05078795924782753,
-0.03904315456748009,
-0.04801316559314728,
-0.044939182698726654,
0.07001601159572601,
-0.015368112362921238
] |
PMID:25149 | The use of gamma-glutamyl-p-aminobenzoic acid as the substrate for determination of gamma-glutamyltranspeptidase activity in blood serum. | By the phthaloyl method less toxic and readily soluble gamma-L-glutamyl-p-aminobenzoic acid was synthesized. This substance was used as a substrate for gamma-glutamyltranspeptidase activity assay in blood serum and urine. Close correlation was shown between the results obtained with the new method and with the old one which used gamma-L-glutamyl-p-nitroanilide. | The use of gamma-glutamyl-p-aminobenzoic acid as the substrate for determination of gamma-glutamyltranspeptidase activity in blood serum. By the phthaloyl method less toxic and readily soluble gamma-L-glutamyl-p-aminobenzoic acid was synthesized. This substance was used as a substrate for gamma-glutamyltranspeptidase activity assay in blood serum and urine. Close correlation was shown between the results obtained with the new method and with the old one which used gamma-L-glutamyl-p-nitroanilide. | [
-0.0008148616761900485,
0.03375096991658211,
0.0037536825984716415,
-0.02749074064195156,
0.02395802177488804,
0.01563168130815029,
0.07026535272598267,
0.06684570014476776,
-0.07342623919248581,
-0.014992146752774715,
0.023230208083987236,
-0.03702611103653908,
0.016919394955039024,
0.04063303768634796,
-0.08060670644044876,
-0.0608179047703743,
0.01715944893658161,
0.008950717747211456,
-0.00522275548428297,
-0.02781977504491806,
-0.004702679812908173,
0.022510675713419914,
0.061413198709487915,
-0.014866826124489307,
0.048127301037311554,
0.017660142853856087,
-0.03472055867314339,
-0.02522709034383297,
-0.04564070329070091,
-0.14068254828453064,
0.024145765230059624,
-0.019075242802500725,
-0.004555596970021725,
-0.03812023624777794,
-0.04811178520321846,
0.02228422276675701,
-0.04329565167427063,
0.0042994157411158085,
0.001236475887708366,
0.027615318074822426,
0.03742996230721474,
0.046462532132864,
-0.03544740751385689,
0.010106606408953667,
0.060808271169662476,
0.012846825644373894,
-0.027919676154851913,
0.028060095384716988,
0.038768962025642395,
0.05670957267284393,
-0.000003467775968601927,
0.01526038907468319,
0.08518873900175095,
0.0023837187327444553,
-0.017414184287190437,
-0.020043114200234413,
0.006188560742884874,
0.0069187963381409645,
-0.04815824702382088,
0.06280387938022614,
0.014000223949551582,
-0.024685189127922058,
-0.14379185438156128,
0.03374422341585159,
0.04917217046022415,
0.03409348055720329,
0.002831515856087208,
-0.04455719143152237,
0.04997355863451958,
0.05052169784903526,
-0.025632651522755623,
0.011592733673751354,
-0.001548619708046317,
0.0317976251244545,
-0.021391836926341057,
0.04944106191396713,
0.0339583121240139,
0.0024284084793180227,
-0.00012181505735497922,
0.020952818915247917,
0.011513731442391872,
0.04441189393401146,
0.03912905231118202,
0.010965644381940365,
0.037326425313949585,
-0.04204704985022545,
-0.005430884193629026,
0.028965994715690613,
0.0432065986096859,
-0.005768148694187403,
-0.033302873373031616,
-0.07917335629463196,
-0.04283067584037781,
-0.02005922421813011,
-0.06379105150699615,
-0.016770778223872185,
-0.0020675419364124537,
0.008504899218678474,
0.024102581664919853,
0.38609543442726135,
-0.006465881131589413,
0.006893127225339413,
-0.10822159796953201,
0.005778621416538954,
0.0013651639455929399,
-0.014116987586021423,
-0.03419312462210655,
-0.06761212646961212,
-0.02778767980635166,
0.022601529955863953,
0.009286778047680855,
0.0238826721906662,
0.019534409046173096,
-0.03545895218849182,
-0.030724147334694862,
-0.004028331954032183,
0.010573944076895714,
0.03909070044755936,
-0.04297441244125366,
0.03880856931209564,
0.013802547007799149,
-0.0056046065874397755,
-0.006599460728466511,
-0.05132702738046646,
0.03691011294722557,
0.03166711702942848,
-0.04602721333503723,
0.035587482154369354,
0.06217440962791443,
0.049483440816402435,
0.04205579310655594,
-0.0015453489031642675,
0.001972390338778496,
0.022624541074037552,
0.026089197024703026,
0.02139408327639103,
0.03469930216670036,
-0.002127828076481819,
0.060939837247133255,
-0.006320612970739603,
-0.054485782980918884,
-0.017444681376218796,
-0.09620977193117142,
-0.033147186040878296,
-0.042047109454870224,
0.08322988450527191,
0.004540328402072191,
0.0428016372025013,
0.04761155694723129,
0.013865198940038681,
0.015221094712615013,
0.04462916776537895,
0.025715019553899765,
0.042353708297014236,
-0.0375826433300972,
0.006278453394770622,
0.015091617591679096,
0.02090647630393505,
-0.00026183726731687784,
0.02455630712211132,
-0.1047276183962822,
-0.047419868409633636,
-0.0003319852112326771,
0.051046065986156464,
0.012428434565663338,
0.0038053507450968027,
-0.039268799126148224,
-0.027231687679886818,
-0.007127417251467705,
-0.036215122789144516,
0.010464034043252468,
0.05893959850072861,
0.009421137161552906,
-0.015844270586967468,
-0.05986694619059563,
0.06276261806488037,
0.05615556985139847,
0.05451824143528938,
0.025131430476903915,
0.02911805361509323,
0.059644024819135666,
-0.011697635054588318,
-0.07896123826503754,
0.023620430380105972,
-0.015438857488334179,
-0.035101354122161865,
-0.007214573211967945,
0.0027105314657092094,
0.012679104693233967,
0.02823830209672451,
-0.041382983326911926,
-0.010562017560005188,
-0.07498320192098618,
0.025125980377197266,
-0.007915955036878586,
-0.05909641087055206,
-0.07688196003437042,
0.009024844504892826,
0.03697889670729637,
0.001341147581115365,
0.1362358182668686,
0.03198891133069992,
-0.021186228841543198,
0.033230140805244446,
-0.004591506440192461,
0.008609400130808353,
-0.025651803240180016,
-0.020104406401515007,
0.016901126131415367,
0.05582050234079361,
0.01840139552950859,
0.03361527994275093,
-0.05454724654555321,
0.04776538908481598,
-0.04819374904036522,
0.03035890683531761,
-0.018430665135383606,
0.0008683038759045303,
0.011606794781982899,
0.029370605945587158,
-0.04747866094112396,
-0.03261132165789604,
-0.01378054078668356,
-0.3228437006473541,
-0.01468893326818943,
0.030437903478741646,
-0.03846108913421631,
0.027827614918351173,
-0.08018134534358978,
0.005102341994643211,
-0.07533640414476395,
-0.03442548215389252,
0.010303319431841373,
0.08903983235359192,
0.07924885302782059,
-0.02901465632021427,
-0.0905950739979744,
0.021243836730718613,
0.03997956961393356,
-0.038696493953466415,
-0.0485871396958828,
0.03272700682282448,
-0.0008495767833665013,
0.056493185460567474,
-0.07697682827711105,
0.01935146562755108,
0.0063362945802509785,
0.07128271460533142,
-0.02539278008043766,
0.14095860719680786,
-0.0036917664110660553,
-0.004325238987803459,
-0.022609876468777657,
0.001659702742472291,
0.01029997132718563,
-0.0407659187912941,
-0.08917795866727829,
-0.011981245130300522,
-0.006156016141176224,
-0.051102735102176666,
-0.0019956924952566624,
0.009582415223121643,
-0.03065735660493374,
-0.016589708626270294,
-0.02370113506913185,
0.008527160622179508,
-0.10300397872924805,
-0.03759108856320381,
-0.051981620490550995,
0.018594887107610703,
-0.02464420720934868,
0.02164289355278015,
0.011907119303941727,
0.022484254091978073,
0.009428972378373146,
0.03810872510075569,
0.019406886771321297,
0.01582389883697033,
0.011691114865243435,
-0.02169647440314293,
0.056913312524557114,
-0.028269384056329727,
0.04568621516227722,
0.01119594182819128,
0.05640232935547829,
0.012842812575399876,
0.04233262315392494,
0.02381926029920578,
-0.004450361710041761,
0.014000630006194115,
-0.026421094313263893,
0.11248613148927689,
0.06114445626735687,
-0.0012132389238104224,
0.09353311359882355,
-0.022325977683067322,
0.06285951286554337,
0.04253561422228813,
0.008321868255734444,
-0.023770000785589218,
-0.03358214721083641,
-0.05842797830700874,
-0.056445956230163574,
-0.09744397550821304,
-0.0212433859705925,
0.0366685576736927,
-0.0040873088873922825,
-0.02069493569433689,
0.004363019485026598,
0.025356793776154518,
-0.019957132637500763,
0.012300632894039154,
0.03603444620966911,
0.03976377844810486,
0.014695409685373306,
-0.048064637929201126,
-0.031840886920690536,
-0.03399397432804108,
0.0075699579901993275,
-0.27344822883605957,
0.004857120104134083,
-0.030288144946098328,
-0.04445892944931984,
-0.030066806823015213,
-0.010091004893183708,
0.03966214507818222,
-0.0074668508023023605,
-0.028872238472104073,
0.03661799803376198,
0.017746657133102417,
0.026040662080049515,
0.021351726725697517,
0.031243059784173965,
0.005777047947049141,
-0.03607641160488129,
0.021222377195954323,
0.0037325252778828144,
0.03236901015043259,
-0.010457945056259632,
0.051737502217292786,
-0.025886476039886475,
0.15792174637317657,
0.0434248186647892,
-0.0064076329581439495,
0.06465456634759903,
0.0020532666239887476,
-0.06913716346025467,
0.024208413437008858,
0.03473436459898949,
-0.020363977178931236,
-0.042728736996650696,
0.0856829360127449,
-0.08332784473896027,
-0.004869222175329924,
0.09849863499403,
-0.019806865602731705,
0.00888027437031269,
-0.05476504564285278,
-0.008175687864422798,
0.0031791601795703173,
0.02319376915693283,
-0.034912943840026855,
-0.021658383309841156,
0.07890111953020096,
-0.05035950988531113,
-0.039243657141923904,
-0.07500268518924713,
0.002602629130706191,
-0.0008831781451590359,
-0.04429863020777702,
-0.03524819016456604,
-0.037879038602113724,
-0.021001338958740234,
0.021741222590208054,
-0.024240728467702866,
-0.0195934996008873,
0.0070150019600987434,
-0.0032814948353916407,
-0.01620175875723362,
-0.016095703467726707,
0.007999522611498833,
-0.06420453637838364,
0.06985215097665787,
-0.0518425889313221
] |
PMID:25150 | The effect of urinary pH and flow rate on monoamine output. | Three-hour urinary output values of dopamine, noradrenaline, adrenaline, 2-phenylethylamine and p-tyramine were measured in normal volunteers who had been induced, by pretreatment with ammonium chloride or sodium bicarbonate, to excrete maximally acid or alkaline urine. There were significant effects on the excretion of dopamine, adrenaline and 2-phenylethylamine, inversely proportional to urinary pH value. Adrenaline output increased with increasing urinary flow rate. There was a significant correlation between urinary concentrations of 2-phenylethylamine and p-tyramine. These findings may have important clinical implications. | The effect of urinary pH and flow rate on monoamine output. Three-hour urinary output values of dopamine, noradrenaline, adrenaline, 2-phenylethylamine and p-tyramine were measured in normal volunteers who had been induced, by pretreatment with ammonium chloride or sodium bicarbonate, to excrete maximally acid or alkaline urine. There were significant effects on the excretion of dopamine, adrenaline and 2-phenylethylamine, inversely proportional to urinary pH value. Adrenaline output increased with increasing urinary flow rate. There was a significant correlation between urinary concentrations of 2-phenylethylamine and p-tyramine. These findings may have important clinical implications. | [
-0.036608971655368805,
0.02482963725924492,
-0.001117125153541565,
0.06945624202489853,
-0.018469244241714478,
0.016793712973594666,
0.09190671145915985,
0.035655584186315536,
-0.010511664673686028,
-0.02663545124232769,
-0.003385137999430299,
-0.036971814930438995,
0.014775868505239487,
0.08624470978975296,
-0.02072841301560402,
0.00130466150585562,
0.04175110161304474,
-0.009181713685393333,
-0.051602549850940704,
-0.04024341329932213,
0.03235704079270363,
-0.039671819657087326,
0.056744109839200974,
-0.026505105197429657,
0.03160647675395012,
-0.04395700991153717,
-0.04424331337213516,
-0.023762313649058342,
-0.016842497512698174,
-0.1544428914785385,
0.0472269281744957,
0.01805751584470272,
-0.019772104918956757,
-0.05789746344089508,
0.011371253989636898,
0.03090287186205387,
-0.03998773172497749,
0.016773290932178497,
-0.018023472279310226,
0.05234503373503685,
-0.004551648627966642,
0.03931010514497757,
0.015251901932060719,
0.029468586668372154,
0.009253774769604206,
0.014268585480749607,
-0.07136747986078262,
-0.00023018351930659264,
0.1470842808485031,
0.0388740636408329,
0.010908395983278751,
-0.008965907618403435,
0.04093509912490845,
0.01011611707508564,
0.04531412571668625,
-0.04870479553937912,
0.0007470895652659237,
0.030351882800459862,
-0.02472563274204731,
0.020107777789235115,
-0.008599660359323025,
0.05497288703918457,
-0.17405319213867188,
0.042164452373981476,
0.060575373470783234,
-0.0072361016646027565,
-0.07582862675189972,
0.04025120288133621,
0.05550529435276985,
0.003591383108869195,
-0.06255875527858734,
0.05094793066382408,
-0.018537450581789017,
-0.04878577962517738,
0.041225749999284744,
-0.011653771623969078,
-0.009336304850876331,
-0.03296015039086342,
-0.00015564332716166973,
-0.026195760816335678,
0.017193321138620377,
0.003436570754274726,
0.01428125984966755,
-0.03386856988072395,
0.06689996272325516,
-0.030000662431120872,
0.02604272961616516,
0.03607802465558052,
-0.0349537692964077,
-0.032783448696136475,
-0.0140702398493886,
-0.045072369277477264,
-0.04687349498271942,
0.04434792697429657,
-0.007059766445308924,
0.007884583435952663,
-0.013179242610931396,
-0.048717230558395386,
0.003503438550978899,
0.3782111704349518,
-0.007364910561591387,
0.03254753351211548,
-0.04473980516195297,
-0.02111896686255932,
0.04554491117596626,
0.012337788939476013,
0.01653338596224785,
-0.013945911079645157,
-0.00806327722966671,
0.0116158127784729,
0.057290710508823395,
0.03969838470220566,
0.06691332906484604,
-0.08578485250473022,
-0.0415421687066555,
-0.011609134264290333,
0.03208053857088089,
0.03902819752693176,
0.0375027060508728,
-0.005984453950077295,
-0.032680898904800415,
0.005118673201650381,
-0.03414791449904442,
-0.0048117004334926605,
0.007942523807287216,
-0.03014560416340828,
0.0573335662484169,
0.10500116646289825,
-0.03839699178934097,
-0.0008121418068185449,
-0.0143761420622468,
0.022114625200629234,
-0.02331630513072014,
0.05738689750432968,
0.007426792755723,
-0.01899554580450058,
0.056245215237140656,
-0.017910271883010864,
0.004369280766695738,
0.05599290505051613,
-0.035988859832286835,
-0.06775356829166412,
-0.05501409247517586,
-0.09832873940467834,
-0.03652186691761017,
0.1370898187160492,
0.04585529863834381,
-0.024945730343461037,
-0.022201305255293846,
-0.004277851432561874,
-0.036574069410562515,
0.05574595555663109,
-0.07556569576263428,
0.03159770742058754,
-0.007456967141479254,
0.018227428197860718,
-0.0013451685663312674,
-0.0219022948294878,
0.016689956188201904,
-0.03290293738245964,
-0.0166611485183239,
-0.03357873484492302,
-0.030494146049022675,
0.037201207131147385,
0.011876905336976051,
0.005066358018666506,
-0.01579008251428604,
-0.045906707644462585,
0.007400535978376865,
0.008298699744045734,
-0.005695904605090618,
0.004878964275121689,
-0.025287309661507607,
-0.014141097664833069,
-0.03264344111084938,
-0.05446653440594673,
0.029538286849856377,
0.05644981935620308,
0.022025179117918015,
-0.034819141030311584,
0.060570843517780304,
0.028195112943649292,
0.026037780568003654,
-0.014937820844352245,
0.02481914311647415,
-0.005089016165584326,
-0.033483460545539856,
0.0017587465699762106,
0.009640471078455448,
0.04233299568295479,
-0.04205275699496269,
-0.029617318883538246,
-0.1222073882818222,
-0.012455231510102749,
-0.051597580313682556,
-0.027974409982562065,
-0.04683908447623253,
0.05602562054991722,
-0.060598134994506836,
0.007820711471140385,
0.08612141013145447,
0.01348402164876461,
0.007704373449087143,
0.02524741180241108,
0.03945459425449371,
0.020823340862989426,
-0.052179913967847824,
0.04075578227639198,
0.03752769157290459,
0.008681297302246094,
-0.002040325663983822,
-0.0005179032450541854,
0.048859916627407074,
-0.01418563537299633,
-0.04614963382482529,
0.04036203771829605,
0.029515067115426064,
0.01017718855291605,
0.0725441500544548,
0.02156790718436241,
0.053827229887247086,
-0.03798917680978775,
-0.02725953981280327,
-0.28462979197502136,
0.01846281811594963,
0.049128372222185135,
-0.015958257019519806,
0.11850860714912415,
-0.08682742714881897,
0.0006476076086983085,
-0.027377774938941002,
-0.022078165784478188,
-0.010240936651825905,
0.026013758033514023,
0.04912250116467476,
-0.00039689941331744194,
-0.005887963809072971,
0.023998036980628967,
0.047976188361644745,
0.04594990983605385,
-0.036585114896297455,
0.02349274605512619,
0.05088682472705841,
0.06273394823074341,
0.0033380293752998114,
0.012027284130454063,
-0.031398773193359375,
0.02117854729294777,
-0.02862754836678505,
0.13138359785079956,
0.010191120207309723,
-0.023683756589889526,
-0.014418540522456169,
-0.017217125743627548,
0.03970831260085106,
-0.053384263068437576,
-0.15084007382392883,
-0.011525142006576061,
-0.036996323615312576,
0.024337828159332275,
0.005061109084635973,
-0.03751945495605469,
-0.041239067912101746,
-0.10033547133207321,
-0.023423966020345688,
0.03995490446686745,
-0.03836251422762871,
-0.049172673374414444,
-0.003958768676966429,
0.00671734381467104,
0.025047343224287033,
-0.06190948560833931,
-0.013677062466740608,
0.06415197253227234,
0.020055826753377914,
-0.007163166534155607,
-0.06772905588150024,
-0.018429851159453392,
-0.012412137351930141,
-0.0786350816488266,
0.0042463247664272785,
-0.07706169784069061,
-0.05340566486120224,
-0.022397823631763458,
-0.013473758473992348,
0.0605996698141098,
-0.00790812261402607,
0.007461571134626865,
-0.009008275344967842,
0.018942927941679955,
-0.012023087590932846,
0.037608709186315536,
0.05726339668035507,
0.04234028980135918,
0.11356398463249207,
-0.013569646514952183,
0.04534117132425308,
0.06200813874602318,
-0.034297630190849304,
-0.04801828786730766,
-0.019341018050909042,
-0.026576228439807892,
-0.006440944038331509,
-0.0594015009701252,
-0.01693989522755146,
0.05554375797510147,
0.04420429840683937,
0.009958578273653984,
0.03354625403881073,
0.006236205343157053,
-0.04685155302286148,
0.0399770513176918,
0.02994021773338318,
-0.0147330891340971,
0.012526135891675949,
0.04255004972219467,
-0.012683538720011711,
-0.017892608419060707,
0.025212934240698814,
-0.2845633327960968,
-0.017260205000638962,
0.01802545040845871,
-0.04997117444872856,
-0.0053254906088113785,
0.02881576120853424,
0.0379314161837101,
-0.010336922481656075,
-0.001161880325525999,
0.0434892512857914,
0.02430422604084015,
0.027905499562621117,
0.0381697341799736,
0.03501710668206215,
-0.038379669189453125,
0.009802110493183136,
0.021398333832621574,
-0.013126813806593418,
0.009506751783192158,
-0.01194553542882204,
0.031172921881079674,
0.007793908938765526,
0.142713263630867,
0.0037020230665802956,
-0.001638052985072136,
0.024580879136919975,
0.013621838763356209,
0.04788922518491745,
0.008163944818079472,
-0.015436668880283833,
-0.05574875697493553,
0.04404500126838684,
0.056229621171951294,
-0.02870241552591324,
0.03815677389502525,
0.02080044522881508,
-0.07579073309898376,
-0.0336458794772625,
0.0007859044708311558,
0.0022365404292941093,
-0.03265325725078583,
-0.026842990890145302,
-0.03145631030201912,
-0.04619721323251724,
0.07154245674610138,
-0.03488463535904884,
-0.010720220394432545,
-0.04330413416028023,
-0.0360611192882061,
-0.00865334365516901,
-0.0077022043988108635,
-0.039357610046863556,
0.0032895198091864586,
0.04118857532739639,
0.021494336426258087,
0.03400641679763794,
-0.06852523982524872,
-0.06369784474372864,
0.030728021636605263,
-0.012027022428810596,
-0.04628635570406914,
0.00790060218423605,
-0.007211551535874605,
0.04533761739730835,
0.024737877771258354
] |
PMID:25151 | Acid esterase activity in cultured skin fibroblasts and amniotic fluid cells using 4-methylumbelliferyl palmitate. | The properties and levels of acid esterase in cultured skin fibroblasts and amniotic fluid cells were investigated using 4-methylumbelliferyl palmitate as substrate. Determinations of acid esterase activity could be made using as little as 1 microgram cell protein. Cardiolipin increased the activity 2--3 fold at the pH optimum 4.0. The apparent KM for both cell types studied was 196 micrometer without and 96 micrometer with cardiolipin. Acid esterase activity was inhibited by cyanide and thiomersal, but not by iodoacetate and N-ethylmaleimide. However activation by cardiolipin was prevented by iodoacetate, N-ethylmaleimide and also sodium chloride. Skin fibroblasts and primary amniotic fluid cells had similar levels with or without cardiolipin. A cyclic activity was found with subculture but no consistent pattern with passage. The acid esterase deficiency in Wolman's and cholesterol ester storage diseases was demonstrated with this substrate. | Acid esterase activity in cultured skin fibroblasts and amniotic fluid cells using 4-methylumbelliferyl palmitate. The properties and levels of acid esterase in cultured skin fibroblasts and amniotic fluid cells were investigated using 4-methylumbelliferyl palmitate as substrate. Determinations of acid esterase activity could be made using as little as 1 microgram cell protein. Cardiolipin increased the activity 2--3 fold at the pH optimum 4.0. The apparent KM for both cell types studied was 196 micrometer without and 96 micrometer with cardiolipin. Acid esterase activity was inhibited by cyanide and thiomersal, but not by iodoacetate and N-ethylmaleimide. However activation by cardiolipin was prevented by iodoacetate, N-ethylmaleimide and also sodium chloride. Skin fibroblasts and primary amniotic fluid cells had similar levels with or without cardiolipin. A cyclic activity was found with subculture but no consistent pattern with passage. The acid esterase deficiency in Wolman's and cholesterol ester storage diseases was demonstrated with this substrate. | [
-0.04009842872619629,
0.01353473961353302,
-0.030109407380223274,
0.045830175280570984,
0.016886455938220024,
-0.05037696659564972,
0.046738151460886,
0.06516154855489731,
-0.05124349147081375,
-0.038993898779153824,
0.017389824613928795,
-0.055893927812576294,
-0.002587639493867755,
0.03212553635239601,
-0.0386226549744606,
-0.017430586740374565,
0.025939680635929108,
-0.007526551838964224,
-0.014713266864418983,
0.016158437356352806,
0.05773992836475372,
0.0014385092072188854,
0.026825498789548874,
-0.032569121569395065,
0.003119064960628748,
-0.013095149770379066,
-0.006039792206138372,
-0.042075421661138535,
-0.09525749087333679,
-0.15398813784122467,
0.03987644240260124,
0.003037861082702875,
0.050386086106300354,
0.014760120771825314,
0.030020512640476227,
-0.004755706060677767,
0.004082652274519205,
0.005515584722161293,
-0.028339598327875137,
0.02452770806849003,
0.0017094851937144995,
0.013283085078001022,
-0.0173106100410223,
0.007428305223584175,
0.03708476945757866,
-0.004767052363604307,
-0.04755750298500061,
0.054996877908706665,
0.0954597219824791,
-0.005283886566758156,
-0.01313039194792509,
0.008862163871526718,
0.05353577435016632,
0.03704017028212547,
-0.007476917002350092,
-0.01847240887582302,
-0.033734280616045,
0.02043643221259117,
-0.017895212396979332,
-0.005427815951406956,
0.000882068183273077,
0.03939339891076088,
-0.11573367565870285,
0.07711516320705414,
0.013259665109217167,
-0.00846391636878252,
-0.031340163201093674,
-0.05675364285707474,
0.03171803057193756,
0.053332023322582245,
-0.07505965232849121,
0.08047656714916229,
-0.002700751181691885,
-0.0005654570995829999,
0.01886826939880848,
-0.034631989896297455,
0.00946791097521782,
0.02647528238594532,
0.04347908869385719,
-0.03676583990454674,
-0.07588501274585724,
-0.08385398238897324,
0.023960772901773453,
-0.026401391252875328,
0.023041268810629845,
0.025884384289383888,
-0.00387967424467206,
-0.04872250556945801,
-0.005022492725402117,
0.03725554421544075,
-0.029015138745307922,
-0.049151115119457245,
-0.05366284027695656,
-0.0156292375177145,
-0.07052932679653168,
0.04199479520320892,
0.066478431224823,
-0.020404836162924767,
-0.031115233898162842,
0.39999088644981384,
-0.018790284171700478,
-0.03391122445464134,
-0.12246046960353851,
0.0005152142839506269,
-0.05279218405485153,
-0.026385337114334106,
-0.08345004171133041,
-0.0022129861172288656,
0.002474074251949787,
0.02441932074725628,
0.04706154018640518,
0.026038244366645813,
-0.00024446711177006364,
-0.06982570886611938,
-0.02571525052189827,
-0.026149099692702293,
0.04892878234386444,
0.007764545734971762,
0.016446728259325027,
0.02861974388360977,
0.02331569977104664,
-0.00017795349413063377,
0.016878172755241394,
0.0036477898247539997,
0.08533766865730286,
-0.0035779778845608234,
0.026008471846580505,
0.08884119987487793,
0.1053171306848526,
0.022326651960611343,
-0.007690442726016045,
0.06786901503801346,
-0.01591287925839424,
0.03262109309434891,
0.005030771717429161,
0.004279447253793478,
0.010266954079270363,
-0.05216490477323532,
0.02037489414215088,
-0.003361084731295705,
-0.012811239808797836,
-0.031666021794080734,
-0.05481722205877304,
-0.04979908838868141,
-0.015872828662395477,
0.06136159598827362,
-0.004131075460463762,
0.04060782119631767,
0.016285059973597527,
0.03470604494214058,
-0.03869377076625824,
0.05534210428595543,
0.009878762066364288,
0.07937513291835785,
0.01235576719045639,
0.0216541551053524,
-0.010897052474319935,
0.002465147292241454,
-0.027089659124612808,
-0.03056996315717697,
-0.04763859510421753,
-0.009598457254469395,
-0.02701275795698166,
0.09083163738250732,
-0.005384601186960936,
0.021511053666472435,
-0.08248559385538101,
-0.012491374276578426,
0.00121433031745255,
-0.020611299201846123,
-0.004623434040695429,
-0.010938430204987526,
0.01676240935921669,
0.016576487571001053,
-0.040719013661146164,
-0.002092883223667741,
0.03493424504995346,
0.046438731253147125,
0.02655208483338356,
0.017841577529907227,
0.07894925773143768,
0.0064339181408286095,
-0.007745896931737661,
-0.009305941872298717,
-0.008930925279855728,
-0.018133286386728287,
-0.0014287206577137113,
-0.04202115908265114,
0.03033081628382206,
0.023212820291519165,
-0.06296736747026443,
-0.025361204519867897,
-0.06598139554262161,
0.04116436094045639,
-0.07192887365818024,
-0.0797419473528862,
-0.013506084680557251,
0.05981004238128662,
-0.00514518516138196,
-0.03628593310713768,
0.0730414167046547,
0.030057566240429878,
0.023986227810382843,
0.04514966160058975,
0.03261493518948555,
0.07825882732868195,
-0.008175460621714592,
0.032940156757831573,
0.01963765174150467,
-0.00891988817602396,
-0.05772082135081291,
0.04089686647057533,
-0.043745752424001694,
-0.03396065533161163,
-0.03862791880965233,
0.06709820032119751,
-0.019597943872213364,
0.007420653011649847,
0.03390730172395706,
-0.017088212072849274,
-0.0013336234260350466,
-0.010862648487091064,
-0.011853720992803574,
-0.3161975145339966,
0.015774626284837723,
0.02890651486814022,
-0.02420315146446228,
0.03658885881304741,
-0.02013806439936161,
0.01950736530125141,
-0.025950515642762184,
-0.012510962784290314,
-0.09849139302968979,
0.032202333211898804,
0.03677254915237427,
-0.013183007016777992,
0.012472129426896572,
0.03660028055310249,
0.008441537618637085,
-0.024638481438159943,
-0.06593355536460876,
0.05259561166167259,
0.001580555341206491,
0.009524471126496792,
-0.034257445484399796,
0.0508546307682991,
0.05057016387581825,
0.1356457620859146,
-0.04268140718340874,
0.08227696269750595,
0.025521328672766685,
-0.015673620626330376,
-0.05116584897041321,
0.038167908787727356,
0.05099921673536301,
-0.040023792535066605,
-0.07754050940275192,
-0.01825280487537384,
-0.027264336124062538,
0.04194272682070732,
-0.016896836459636688,
0.03190413862466812,
0.00036578753497451544,
-0.013390613719820976,
-0.03541308641433716,
-0.023062199354171753,
-0.05238470807671547,
-0.09205541014671326,
0.015067549422383308,
0.018224505707621574,
0.02415195293724537,
-0.04861273244023323,
0.00046178189222700894,
0.017006097361445427,
-0.0034415319096297026,
0.02477998100221157,
0.01592603512108326,
0.014523563906550407,
0.009256052784621716,
-0.014259908348321915,
0.00017410037980880588,
-0.09818005561828613,
-0.010992424562573433,
-0.03184965252876282,
-0.02287062257528305,
0.0324421189725399,
0.032440099865198135,
-0.003789952490478754,
-0.022365644574165344,
-0.0029461944941431284,
-0.0026583464350551367,
0.07302260398864746,
-0.01910378225147724,
0.0041540274396538734,
0.08838465809822083,
0.00028434235719032586,
-0.02176538109779358,
0.030335111543536186,
0.014993194490671158,
0.0062065026722848415,
-0.05691099539399147,
-0.08390695601701736,
0.0008386964909732342,
-0.014789218083024025,
-0.05183776840567589,
0.02385970763862133,
-0.09083566069602966,
-0.02168937399983406,
0.04758981242775917,
0.05210522562265396,
-0.009408465586602688,
0.04105541110038757,
0.0236703772097826,
0.05361984670162201,
-0.020559784024953842,
-0.012145303189754486,
0.008511323481798172,
0.04901077598333359,
-0.03233792260289192,
-0.25904232263565063,
-0.023409973829984665,
0.06529219448566437,
0.021783828735351562,
-0.022417346015572548,
-0.020881520584225655,
0.05703039839863777,
0.01999645121395588,
0.0028365205507725477,
0.054886601865291595,
-0.00253396388143301,
-0.03225702792406082,
0.0760570615530014,
0.07085505872964859,
-0.014671893790364265,
-0.031343381851911545,
0.02425173856317997,
-0.024464232847094536,
0.010155834257602692,
-0.08283479511737823,
-0.01624743826687336,
-0.06616714596748352,
0.12554697692394257,
0.0030079171992838383,
0.01952146738767624,
0.004238083027303219,
-0.030286366119980812,
0.036176327615976334,
-0.03291171416640282,
0.00020907660655211657,
-0.00692908838391304,
0.015796780586242676,
0.0003842666046693921,
0.003950968384742737,
0.035581134259700775,
0.05624741315841675,
0.016260648146271706,
-0.028661789372563362,
-0.016109125688672066,
0.03756581246852875,
0.04584221541881561,
-0.0202742088586092,
-0.10951806604862213,
-0.03908935934305191,
0.010915315710008144,
-0.05445658415555954,
-0.0297770407050848,
-0.0498846173286438,
0.022814173251390457,
0.03140873461961746,
-0.0246462170034647,
-0.045113932341337204,
0.06867249310016632,
0.04855062812566757,
0.035002756863832474,
0.012116247788071632,
-0.02812541089951992,
-0.03748304024338722,
-0.027921287342905998,
0.05390182510018349,
-0.002787853591144085,
-0.008501064963638783,
-0.008546480908989906,
0.1267896443605423,
0.06623811274766922
] |
PMID:25152 | Cardiac and bronchial beta-adrenoceptor antagonistic potencies of atenolol, metoprolol, acebutolol, practolol, propranolol and pindolol in the anaesthetized dog. | 1. The beta-adrenoceptor antagonists atenolol, metoprolol, acebutolol, practolol, propranolol and pindolol have been tested for their ability to reduce isoprenaline-induced bronchodilation and tachycardia in the anaesthetized dog. 2. Atenolol, metoprolol, acebutolol and practolol all possessed a similar degree of cardioselectivity in this animal model. | Cardiac and bronchial beta-adrenoceptor antagonistic potencies of atenolol, metoprolol, acebutolol, practolol, propranolol and pindolol in the anaesthetized dog. 1. The beta-adrenoceptor antagonists atenolol, metoprolol, acebutolol, practolol, propranolol and pindolol have been tested for their ability to reduce isoprenaline-induced bronchodilation and tachycardia in the anaesthetized dog. 2. Atenolol, metoprolol, acebutolol and practolol all possessed a similar degree of cardioselectivity in this animal model. | [
-0.04660787060856819,
0.014982103370130062,
0.0034812409430742264,
0.027514604851603508,
0.010408167727291584,
-0.02694239839911461,
0.027589524164795876,
-0.01478816382586956,
-0.03565278276801109,
-0.03905452787876129,
-0.043385013937950134,
-0.04319116100668907,
0.002747898455709219,
0.05137021839618683,
0.00030755228362977505,
0.0083448626101017,
0.07667487114667892,
0.0033188452944159508,
-0.054496172815561295,
0.0013814126141369343,
-0.0382700115442276,
-0.0009503157925792038,
-0.00019913227879442275,
0.002477790229022503,
0.020920589566230774,
0.04550040513277054,
-0.03779582679271698,
0.004868237301707268,
-0.026704838499426842,
-0.12029802054166794,
-0.006996796000748873,
0.004284634720534086,
0.029109755530953407,
-0.012296941131353378,
-0.0356815941631794,
-0.0002882268454413861,
0.005756288301199675,
0.06068543717265129,
-0.04310682415962219,
0.04594495892524719,
-0.0049121505580842495,
-0.002346803667023778,
-0.004921254701912403,
0.011762084439396858,
-0.0189417265355587,
-0.029130909591913223,
-0.020193232223391533,
-0.031969279050827026,
0.10604491084814072,
-0.022354859858751297,
0.018701136112213135,
-0.015753241255879402,
-0.03674064949154854,
0.0483243502676487,
-0.001656323322094977,
-0.05291333422064781,
-0.07587485015392303,
0.02199600078165531,
-0.018036529421806335,
0.02272704616189003,
-0.03723897412419319,
0.04109007492661476,
-0.12824708223342896,
0.07591923326253891,
-0.011830532923340797,
0.08822080492973328,
-0.018810613080859184,
0.002795896725729108,
0.02425677515566349,
0.10387729108333588,
0.031254906207323074,
-0.0032921498641371727,
-0.007217359729111195,
0.021055884659290314,
0.01718336157500744,
-0.025027712807059288,
-0.00403035432100296,
-0.0021162759512662888,
-0.02096717059612274,
-0.00466069346293807,
0.02362615428864956,
0.027735311537981033,
-0.06459856778383255,
-0.007284334860742092,
0.01488722674548626,
-0.03189822658896446,
0.09362807869911194,
-0.025148525834083557,
-0.006957434583455324,
-0.006545254960656166,
-0.013965086080133915,
-0.04138009622693062,
0.01841699331998825,
0.048630230128765106,
0.01851506344974041,
0.01601024530827999,
-0.016642577946186066,
-0.05094533786177635,
-0.026565177366137505,
0.326059490442276,
0.018117189407348633,
0.07097134739160538,
-0.08439680188894272,
-0.016992023214697838,
0.03399527817964554,
-0.021390311419963837,
-0.006291283294558525,
-0.010322692804038525,
-0.03947242721915245,
0.04572373628616333,
0.02280300296843052,
-0.05713706463575363,
0.043247755616903305,
-0.04923960193991661,
-0.01164498645812273,
-0.06382521986961365,
0.05186326429247856,
-0.026424573734402657,
-0.0022362025920301676,
-0.0008373545133508742,
0.0031762057915329933,
-0.015900811180472374,
0.046053044497966766,
0.059228647500276566,
0.02002895623445511,
-0.0263197124004364,
-0.02587069757282734,
0.11571688950061798,
0.024673793464899063,
0.051241446286439896,
0.054164666682481766,
0.05484993755817413,
-0.014429880306124687,
0.010642078705132008,
0.04944726824760437,
-0.02227744460105896,
0.06572073698043823,
-0.011322868056595325,
0.011761688627302647,
-0.01614455319941044,
0.008783731609582901,
-0.07697727531194687,
0.10228143632411957,
-0.09517430514097214,
-0.0918823853135109,
0.0900738537311554,
0.002421889454126358,
-0.016974661499261856,
-0.08569110929965973,
0.06169823184609413,
-0.07677238434553146,
0.010013215243816376,
0.013431346975266933,
0.09057103842496872,
-0.04719063267111778,
-0.007238186430186033,
0.043771907687187195,
0.05350637808442116,
0.0001799221063265577,
-0.028344644233584404,
-0.08463648706674576,
-0.03583214059472084,
-0.03884453326463699,
0.02102375216782093,
-0.056476399302482605,
-0.018336869776248932,
-0.062005672603845596,
0.007709211204200983,
-0.026242829859256744,
0.07467737793922424,
-0.018794668838381767,
0.02208804525434971,
0.020523279905319214,
-0.03475291654467583,
-0.0439300611615181,
0.038655783981084824,
0.06783636659383774,
-0.02785966545343399,
0.0326286144554615,
-0.05878559499979019,
0.023029325529932976,
-0.009526601061224937,
0.030141744762659073,
-0.03240630403161049,
-0.023421945050358772,
0.053954459726810455,
-0.03827088326215744,
-0.032326530665159225,
0.0051429239101707935,
0.07783079147338867,
-0.02219654619693756,
-0.016093270853161812,
-0.06909679621458054,
-0.01066923514008522,
-0.0414123460650444,
-0.07838234305381775,
-0.07067381590604782,
0.02023421972990036,
-0.014885542914271355,
0.035022709518671036,
0.06087663024663925,
0.030655765905976295,
-0.016659434884786606,
0.01249217800796032,
0.007439070381224155,
0.06295040994882584,
-0.040299490094184875,
-0.0058038863353431225,
0.07219082117080688,
-0.012938535772264004,
0.017160268500447273,
0.051255013793706894,
0.0435604602098465,
0.0370846688747406,
-0.06630578637123108,
0.04076167568564415,
-0.07288262993097305,
-0.04158047214150429,
0.04994932562112808,
0.03327346220612526,
0.005622112657874823,
0.010328222997486591,
0.07230500131845474,
-0.3277494013309479,
-0.03786007687449455,
-0.041098859161138535,
-0.06383576989173889,
0.030621662735939026,
-0.035793256014585495,
0.03871629759669304,
-0.06007228419184685,
-0.06982210278511047,
-0.052950840443372726,
-0.002820161171257496,
-0.0157316867262125,
0.024661310017108917,
0.014987804926931858,
0.008666802197694778,
0.08623827248811722,
0.008799571543931961,
-0.07223327457904816,
-0.001937050255946815,
0.021395770832896233,
0.021995173767209053,
-0.050678692758083344,
-0.057575568556785583,
0.00990403350442648,
0.04925907403230667,
0.034729450941085815,
0.0868806466460228,
0.007489828858524561,
-0.019819417968392372,
-0.006494478788226843,
0.024118443951010704,
-0.010326756164431572,
0.02685500681400299,
-0.059398259967565536,
0.0293778907507658,
-0.0015304973348975182,
0.0849495679140091,
0.01562029030174017,
0.07962997257709503,
-0.0011853048345074058,
-0.0414142906665802,
0.027997517958283424,
-0.004993294831365347,
-0.02333773858845234,
-0.05505064129829407,
0.00338134472258389,
-0.041034504771232605,
0.0815039649605751,
-0.058973487466573715,
0.01777706667780876,
-0.015200444497168064,
0.031961239874362946,
-0.013738214038312435,
-0.061793163418769836,
0.038602136075496674,
0.012306001037359238,
-0.04591066390275955,
-0.027734505012631416,
0.006891233380883932,
-0.0007493730518035591,
0.013532345183193684,
-0.026136647909879684,
0.024816257879137993,
-0.00414372980594635,
-0.03640215843915939,
0.03625088557600975,
0.03880264237523079,
0.01292273961007595,
0.06167832389473915,
0.024778587743639946,
0.005834569223225117,
0.08132200688123703,
-0.04492318630218506,
-0.034026261419057846,
0.06065882742404938,
0.0005988418124616146,
-0.009104731492698193,
-0.06093888729810715,
-0.04729754850268364,
0.004302189685404301,
-0.008754126727581024,
-0.016607964411377907,
0.024655716493725777,
-0.016808753833174706,
-0.04115702211856842,
-0.01939590834081173,
0.0032231491059064865,
0.05169506371021271,
0.08947935700416565,
-0.02497270703315735,
-0.03900692239403725,
0.03907029330730438,
0.002421214710921049,
-0.021753981709480286,
0.026911484077572823,
0.03364257141947746,
-0.2326555699110031,
0.00744678033515811,
0.08730947971343994,
-0.01613694429397583,
0.008811304345726967,
0.0056672655045986176,
0.05010903254151344,
-0.05043085664510727,
-0.023457244038581848,
-0.054213039577007294,
-0.039159417152404785,
0.03661973401904106,
0.057253893464803696,
0.05259891226887703,
-0.089751698076725,
0.04628828912973404,
0.05189814418554306,
0.009101290255784988,
0.06840397417545319,
-0.03520769253373146,
0.02041327953338623,
-0.03687253221869469,
0.14547574520111084,
-0.008020988665521145,
-0.036359645426273346,
0.05993618816137314,
0.005519889760762453,
0.009814736433327198,
-0.03443443775177002,
0.03940028324723244,
0.019043173640966415,
-0.019488198682665825,
0.05963963270187378,
-0.054574884474277496,
0.0026484935078769922,
0.04073185473680496,
-0.06333311647176743,
0.033734891563653946,
0.012610713951289654,
-0.012509845197200775,
-0.06878713518381119,
0.030734248459339142,
-0.06719334423542023,
-0.04873893782496452,
0.06755515933036804,
-0.06358128786087036,
-0.0949660986661911,
0.023346733301877975,
0.009817809797823429,
0.05213102698326111,
-0.005337726790457964,
-0.025758137926459312,
0.06604935973882675,
0.06436937302350998,
0.034651611000299454,
-0.02881392277777195,
-0.0009982665069401264,
-0.01940787583589554,
-0.0343543216586113,
-0.022290444001555443,
-0.019797220826148987,
-0.04534739628434181,
-0.03076232224702835,
0.10601930320262909,
0.07936795055866241
] |
PMID:25153 | The influence of serotonin on the mitotic rate in the colonic crypt epithelium and in colonic adenocarcinoma in rats. | 1. The mitotic rate in the crypts of Lieberkühn of the descending colon and in dimethylhydrazine-induced adenocarcinomata of the descending colon of rat was measured using a stathmokinetic technique. 2. Intraperitoneal injection of a small dose (10 microgram/kg) of serotonin resulted in an increase in the tumour cell mitotic rate. 3. Blockade of serotonin receptors by 2-bromolysergic acid diethylamide and depletion of tissue serotonin levels following injection of DL-6-fluorotryptophan both result in a decrease in the tumour cell mitotic rate. 4. Treatment with serotonin, 2-bromolysergic acid diethylamide and DL-6-fluorotryptophan were all without effect on the colonic crypt cell mitotic rate. | The influence of serotonin on the mitotic rate in the colonic crypt epithelium and in colonic adenocarcinoma in rats. 1. The mitotic rate in the crypts of Lieberkühn of the descending colon and in dimethylhydrazine-induced adenocarcinomata of the descending colon of rat was measured using a stathmokinetic technique. 2. Intraperitoneal injection of a small dose (10 microgram/kg) of serotonin resulted in an increase in the tumour cell mitotic rate. 3. Blockade of serotonin receptors by 2-bromolysergic acid diethylamide and depletion of tissue serotonin levels following injection of DL-6-fluorotryptophan both result in a decrease in the tumour cell mitotic rate. 4. Treatment with serotonin, 2-bromolysergic acid diethylamide and DL-6-fluorotryptophan were all without effect on the colonic crypt cell mitotic rate. | [
-0.02491222508251667,
-0.030933286994695663,
-0.0018712187884375453,
-0.02070363238453865,
-0.03130896016955376,
0.023399341851472855,
0.023778269067406654,
0.046121448278427124,
-0.0010019964538514614,
-0.060838643461465836,
0.05571546405553818,
-0.045555777847766876,
0.010669832117855549,
0.01505739800632,
-0.06681704521179199,
0.00024937043781392276,
0.06735467165708542,
-0.057088520377874374,
-0.09396311640739441,
-0.033165886998176575,
0.013367165811359882,
-0.0526040680706501,
0.041845183819532394,
-0.02919601835310459,
-0.0018246648833155632,
-0.07941657304763794,
-0.05234181880950928,
-0.021228712052106857,
-0.08555863052606583,
-0.15485788881778717,
0.02812957391142845,
-0.012397966347634792,
0.04709766432642937,
0.015841709449887276,
-0.04509735852479935,
-0.03985373303294182,
-0.046852365136146545,
0.0029718484729528427,
0.006841955240815878,
0.02434420958161354,
0.05077975243330002,
-0.007035349030047655,
-0.009812301024794579,
0.04978135973215103,
0.05155647546052933,
-0.0534820482134819,
-0.02989206463098526,
-0.04972557723522186,
0.07619895040988922,
0.006932465359568596,
-0.018034711480140686,
-0.016979804262518883,
-0.017097657546401024,
0.04993170499801636,
0.0020368550904095173,
0.019681468605995178,
0.032486796379089355,
0.07037246227264404,
0.013757195323705673,
0.03363655135035515,
0.0029238739516586065,
0.06433739513158798,
-0.14480102062225342,
0.046861905604600906,
0.03993937745690346,
-0.05343932658433914,
-0.01937425695359707,
-0.01709340699017048,
0.052822671830654144,
0.07815130800008774,
-0.061407461762428284,
0.03464454784989357,
-0.00046203643432818353,
0.015677040442824364,
-0.008438504301011562,
-0.029409300535917282,
-0.022957034409046173,
-0.053646545857191086,
-0.008677723817527294,
0.02931888960301876,
0.04342057183384895,
0.024997275322675705,
0.0238911472260952,
-0.04783052206039429,
-0.023805666714906693,
-0.04057172313332558,
-0.03064548783004284,
-0.054242681711912155,
-0.05311261862516403,
-0.004368944559246302,
-0.014455691911280155,
-0.06619913876056671,
0.03206253424286842,
-0.05626262351870537,
-0.08973813056945801,
-0.006180451717227697,
0.027617376297712326,
0.0011181427398696542,
0.03327043354511261,
0.35449567437171936,
-0.07167967408895493,
0.0072129350155591965,
-0.03610454499721527,
-0.002541533438488841,
0.012858386151492596,
-0.01172601617872715,
-0.02015591226518154,
-0.005055333487689495,
0.04235481098294258,
0.08157716691493988,
0.08491089195013046,
0.03413805738091469,
0.03294029086828232,
0.026016367599368095,
-0.015082311816513538,
-0.0013503317022696137,
0.06876314431428909,
0.03937565162777901,
-0.04098409414291382,
0.04500920698046684,
-0.02173501066863537,
-0.008926687762141228,
0.04447071999311447,
0.02872159145772457,
0.056430526077747345,
0.021470932289958,
-0.0363343320786953,
0.06855665147304535,
0.07497948408126831,
-0.0355946384370327,
0.013398177921772003,
0.06931721419095993,
-0.00320321973413229,
0.02505524642765522,
0.03837191313505173,
-0.028437742963433266,
-0.012124407105147839,
-0.05497727543115616,
0.03165043890476227,
-0.05476359277963638,
-0.02056652493774891,
-0.10484881699085236,
0.03612208366394043,
-0.06680438667535782,
-0.059990253299474716,
0.0923069640994072,
-0.0009377055685035884,
-0.023604154586791992,
-0.028666893020272255,
0.017909053713083267,
-0.004902377258986235,
0.042567092925310135,
0.00638448866084218,
0.02780258096754551,
-0.03989371657371521,
0.06967645138502121,
0.02211775816977024,
0.012241167947649956,
-0.052838586270809174,
-0.021761732175946236,
-0.029757685959339142,
0.02850136160850525,
-0.025415481999516487,
0.07643269747495651,
0.03234026953577995,
0.025723006576299667,
-0.04576275870203972,
-0.012554658576846123,
-0.01797519251704216,
-0.015714094042778015,
-0.01801981031894684,
0.02149016223847866,
-0.0056669930927455425,
-0.012875990942120552,
-0.0230411347001791,
-0.021269062533974648,
0.0410948283970356,
0.004726501647382975,
0.026687726378440857,
0.022330457344651222,
-0.006250480655580759,
-0.05504411831498146,
0.038712240755558014,
0.0856708437204361,
-0.029188673943281174,
0.03347764536738396,
-0.070747010409832,
-0.0009255039622075856,
0.05946148931980133,
-0.008055021986365318,
-0.006042697001248598,
0.008665932342410088,
-0.08856472373008728,
0.03224530816078186,
-0.07584410160779953,
-0.07934722304344177,
-0.032422371208667755,
0.03291482478380203,
-0.056795068085193634,
-0.03675849363207817,
0.05320262908935547,
0.0035655114334076643,
0.03933975100517273,
0.03263130411505699,
0.09040602296590805,
0.05102362856268883,
0.008867964148521423,
0.036217860877513885,
0.04854808375239372,
-0.021950332447886467,
-0.024811727926135063,
0.0023249899968504906,
0.0407969132065773,
0.026828570291399956,
-0.052881259471178055,
0.11264941841363907,
0.01284897793084383,
-0.023982835933566093,
0.07860804349184036,
-0.0180235356092453,
0.029543055221438408,
-0.023476654663681984,
-0.03733370080590248,
-0.32479235529899597,
-0.051241181790828705,
-0.013769430108368397,
-0.018040457740426064,
0.03877861797809601,
-0.03510499745607376,
-0.03761526942253113,
-0.05719242990016937,
-0.024968693032860756,
-0.06685254722833633,
0.01788998581469059,
0.037383414804935455,
-0.01995684765279293,
-0.013516542501747608,
0.019728098064661026,
-0.0020737170707434416,
-0.03267844393849373,
-0.0048880609683692455,
0.025902437046170235,
0.013517213985323906,
-0.006172489374876022,
-0.011612915433943272,
-0.0060089402832090855,
-0.0010163122788071632,
0.02487782947719097,
-0.030851854011416435,
0.10803093761205673,
0.018207764253020287,
-0.013993999920785427,
0.028273357078433037,
0.035522397607564926,
0.01988173834979534,
-0.03646264225244522,
-0.0945754200220108,
-0.001408091397024691,
-0.00861053355038166,
0.025197245180606842,
-0.01999550312757492,
-0.05945347249507904,
-0.008953017182648182,
-0.048625409603118896,
0.011905370280146599,
-0.002118981210514903,
-0.04740290716290474,
-0.03279774636030197,
-0.01847025379538536,
0.032288480550050735,
0.02901965193450451,
-0.01816411130130291,
-0.05918952822685242,
0.027295369654893875,
-0.004298631567507982,
0.021162381395697594,
-0.05047018453478813,
0.0416531078517437,
-0.008938589133322239,
-0.031482160091400146,
-0.022209040820598602,
-0.06845097988843918,
0.012954146601259708,
0.05312422290444374,
0.0014756526798009872,
0.09074527025222778,
-0.019637538120150566,
0.023280970752239227,
0.019119134172797203,
-0.02492367848753929,
0.00481457682326436,
0.04902096465229988,
0.010473414324223995,
0.04836300015449524,
0.11710714548826218,
-0.019417788833379745,
0.03305654227733612,
0.028670618310570717,
-0.01598156988620758,
-0.03631279617547989,
-0.055222004652023315,
-0.11099863052368164,
0.021135516464710236,
0.02926114946603775,
-0.05066867917776108,
0.045217569917440414,
0.00951943825930357,
-0.05916067957878113,
-0.00386187550611794,
0.01790541224181652,
0.005806894972920418,
0.025460273027420044,
0.0019875236321240664,
0.021120483055710793,
-0.007355989422649145,
0.03233803063631058,
-0.007043167948722839,
0.006355418357998133,
0.03413345292210579,
-0.3037737309932709,
0.03294739872217178,
-0.005675054620951414,
-0.020913442596793175,
-0.024152951315045357,
0.011831630952656269,
0.062507264316082,
0.02705967053771019,
-0.013247703202068806,
0.04046317934989929,
0.004336266778409481,
0.006410953123122454,
0.03346537798643112,
0.022008251398801804,
-0.005640266928821802,
-0.006029588636010885,
-0.013928054831922054,
-0.019790425896644592,
-0.023744598031044006,
0.014647047966718674,
0.057178910821676254,
-0.045074574649333954,
0.14902108907699585,
0.01180970761924982,
0.006581947207450867,
0.06248939782381058,
0.05395844206213951,
0.07469676434993744,
0.034433212131261826,
0.00817587599158287,
0.05361652746796608,
0.02976924180984497,
0.012088023126125336,
0.0012459808494895697,
0.02765953168272972,
0.0019926633685827255,
-0.006921415217220783,
0.011791810393333435,
-0.037279725074768066,
0.0298023484647274,
-0.023859720677137375,
0.05299588292837143,
-0.03567313402891159,
0.03346654400229454,
0.05954062566161156,
-0.0627637580037117,
-0.004262670874595642,
-0.0631047934293747,
0.003033548826351762,
0.04191197454929352,
0.0020260957535356283,
0.019252941012382507,
0.022841699421405792,
0.04908524826169014,
0.02588348090648651,
-0.027622461318969727,
-0.02867092378437519,
-0.03145045042037964,
0.03065241500735283,
-0.00006643815868301317,
0.013036208227276802,
-0.042700402438640594,
-0.04548818990588188,
0.06555196642875671,
-0.0063635557889938354
] |
PMID:25157 | Kinetics, salivary excretion of amphetamine isomers, and effect of urinary pH. | Amphetamine was administered to healthy subjects as the racemic mixture and as (+)- and (-)-isomers under conditions of urine acidification and alkalinization. Plasma and saliva concentration of each isomer was measured and the kinetics of the individual isomers were determined. (+)-amphetamine was eliminated more rapidly than the (-)-isomer. The difference in half-life between isomers was maximal under basic urinary pH conditions. Saliva amphetamine levels were higher than plasma levels and in the postabsorptive phase were predictably proportional to plasma drug levels. | Kinetics, salivary excretion of amphetamine isomers, and effect of urinary pH. Amphetamine was administered to healthy subjects as the racemic mixture and as (+)- and (-)-isomers under conditions of urine acidification and alkalinization. Plasma and saliva concentration of each isomer was measured and the kinetics of the individual isomers were determined. (+)-amphetamine was eliminated more rapidly than the (-)-isomer. The difference in half-life between isomers was maximal under basic urinary pH conditions. Saliva amphetamine levels were higher than plasma levels and in the postabsorptive phase were predictably proportional to plasma drug levels. | [
-0.047216400504112244,
0.012259752489626408,
0.023477530106902122,
0.0009616874158382416,
-0.020175116136670113,
0.031244611367583275,
0.07506059855222702,
0.02304810658097267,
-0.04236259311437607,
-0.015483069233596325,
0.03652318939566612,
-0.03624743968248367,
0.028942802920937538,
0.06631205230951309,
0.013326426036655903,
0.00840460043400526,
0.04154425859451294,
-0.03506065905094147,
-0.005227344110608101,
-0.05469522997736931,
0.036970969289541245,
-0.049092214554548264,
0.06554283946752548,
0.0155280577018857,
-0.0006302210967987776,
0.023274900391697884,
-0.03127167373895645,
-0.00834593828767538,
-0.035058893263339996,
-0.16366608440876007,
0.057820677757263184,
-0.022188778966665268,
-0.044625330716371536,
-0.06609613448381424,
-0.0370345301926136,
0.0209624283015728,
-0.03593872860074043,
0.05969192087650299,
0.007059797644615173,
0.02149459533393383,
0.016311636194586754,
-0.01338740810751915,
-0.026691263541579247,
0.020859386771917343,
-0.0068590533919632435,
0.026288781315088272,
-0.11853785067796707,
0.02756856195628643,
0.13216662406921387,
-0.010177724063396454,
0.005484194029122591,
-0.006327359937131405,
0.03214910998940468,
0.03247745707631111,
0.009317017160356045,
-0.0027477226685732603,
0.05536694452166557,
0.016074232757091522,
-0.03616854548454285,
0.010482716374099255,
-0.0554044172167778,
0.032300595194101334,
-0.13689906895160675,
0.05711311101913452,
0.08536095172166824,
0.012618312612175941,
-0.06243564933538437,
0.0012701580999419093,
0.0316455215215683,
0.05021458864212036,
-0.059126462787389755,
0.06037034094333649,
-0.0065931277349591255,
-0.004462793003767729,
0.00959012471139431,
-0.02551349624991417,
0.03909015655517578,
0.023008059710264206,
0.012736795470118523,
-0.0291745662689209,
0.0024794244673103094,
0.015679195523262024,
-0.003227231791242957,
-0.010146759450435638,
0.025791233405470848,
-0.05216803029179573,
0.05536581948399544,
-0.022026382386684418,
-0.013764146715402603,
0.00565388984978199,
-0.0467379167675972,
-0.03271772712469101,
-0.014635412022471428,
-0.01285834051668644,
0.03464563563466072,
0.020727885887026787,
-0.0029949224554002285,
-0.06213562190532684,
0.04987844452261925,
0.3865795135498047,
-0.033798329532146454,
-0.010187225416302681,
-0.048916175961494446,
-0.05232466757297516,
0.00028144032694399357,
0.007215050049126148,
0.0016555965412408113,
-0.026197904720902443,
-0.014516891911625862,
0.025806143879890442,
0.013773897662758827,
0.06838010996580124,
0.042282335460186005,
-0.045825276523828506,
-0.05110916867852211,
-0.001396578038111329,
-0.009743411093950272,
0.022741559892892838,
0.008400632999837399,
-0.013081477023661137,
-0.04432274401187897,
0.00910805445164442,
0.013069197535514832,
-0.058606065809726715,
0.04090346023440361,
-0.006537873297929764,
0.011992684565484524,
0.12568405270576477,
0.00868925265967846,
0.002023316454142332,
0.022675147280097008,
-0.022003069519996643,
0.0011844690889120102,
0.03667654097080231,
0.01053594145923853,
-0.03925734758377075,
0.02528693713247776,
-0.0033610323444008827,
0.07745181769132614,
0.04386810213327408,
-0.07997991889715195,
-0.024716611951589584,
-0.03374413773417473,
-0.07781648635864258,
-0.023980848491191864,
0.11248908936977386,
-0.045599233359098434,
-0.022714054211974144,
0.008807748556137085,
0.013185559771955013,
-0.016446322202682495,
0.039818111807107925,
-0.04607054963707924,
0.0017660856246948242,
-0.048660870641469955,
-0.002817313652485609,
0.02927587367594242,
-0.026293907314538956,
0.011076588183641434,
0.008777303621172905,
-0.044123414903879166,
-0.06738720834255219,
-0.001778490375727415,
0.0801907554268837,
-0.0233681071549654,
0.013388057239353657,
-0.055062104016542435,
-0.056673452258110046,
-0.0050773159600794315,
-0.004408216569572687,
0.013526806607842445,
-0.07945168763399124,
-0.015752991661429405,
0.03522861376404762,
-0.05522099509835243,
-0.03806660696864128,
0.03960004076361656,
0.027555182576179504,
-0.022912610322237015,
-0.04317115619778633,
-0.01611935719847679,
0.031835004687309265,
-0.02180662751197815,
0.05255184695124626,
0.04978764429688454,
-0.03337257727980614,
-0.04227752983570099,
-0.004886363632977009,
0.015948092564940453,
0.09793147444725037,
0.02643454261124134,
-0.02533816173672676,
-0.1172216460108757,
0.02813929133117199,
0.0048049138858914375,
-0.0015849592164158821,
-0.0728047788143158,
-0.005944248288869858,
-0.026763396337628365,
0.030802134424448013,
0.03118983469903469,
0.03684399649500847,
-0.0009080339223146439,
0.03741661459207535,
0.06415589898824692,
0.06845075637102127,
-0.0050353859551250935,
-0.00394812086597085,
0.052789781242609024,
0.014164377935230732,
-0.015348337590694427,
-0.028330178931355476,
-0.012928355485200882,
0.006702886428683996,
-0.003695714520290494,
0.01247223187237978,
0.02206655777990818,
-0.017213812097907066,
0.05182439461350441,
0.004278178326785564,
-0.030906282365322113,
-0.035120174288749695,
-0.025932665914297104,
-0.2727189064025879,
0.01080174557864666,
0.035757552832365036,
0.025099201127886772,
0.10914835333824158,
-0.06149744614958763,
0.017815399914979935,
-0.030928710475564003,
0.01189151406288147,
-0.02311793528497219,
0.024749601259827614,
0.06757411360740662,
0.010112938471138477,
0.01785067841410637,
0.01604158617556095,
0.05237296223640442,
-0.012518447823822498,
0.00579718966037035,
0.006656118668615818,
-0.019479556009173393,
0.010792805813252926,
0.024981588125228882,
0.007878072559833527,
0.052323415875434875,
0.060287218540906906,
-0.004552996717393398,
0.15534207224845886,
-0.009030808694660664,
-0.0384037122130394,
0.018387742340564728,
-0.03481792286038399,
0.053467512130737305,
-0.013377038761973381,
-0.13020376861095428,
-0.022758811712265015,
-0.04202091321349144,
0.0159225445240736,
-0.0164849404245615,
-0.035970501601696014,
-0.004570845514535904,
-0.07626725733280182,
-0.0031201227102428675,
0.013817721046507359,
-0.04931770637631416,
-0.0635961964726448,
0.019252104684710503,
-0.011049971915781498,
0.009021376259624958,
-0.017911752685904503,
-0.02198112942278385,
0.0792429968714714,
0.002948475070297718,
0.05428486689925194,
-0.04043267294764519,
-0.02640894614160061,
-0.0033470364287495613,
-0.05905906856060028,
0.004566689487546682,
-0.10731309652328491,
0.002393333939835429,
-0.00539082195609808,
-0.016589559614658356,
0.04159065708518028,
0.0005541385035030544,
-0.018090419471263885,
0.0028935952577739954,
0.038032129406929016,
-0.010271982289850712,
0.034213587641716,
0.04768587648868561,
-0.019964545965194702,
0.11431742459535599,
-0.038528233766555786,
0.008617355488240719,
0.000012413952390488703,
-0.00930990744382143,
-0.08116333931684494,
-0.027651071548461914,
-0.06780298799276352,
0.014936039224267006,
-0.04804044961929321,
-0.016288362443447113,
0.05514433607459068,
0.037727613002061844,
0.02572215162217617,
0.01028783991932869,
0.021076401695609093,
0.005505072418600321,
0.02654699608683586,
0.02708636410534382,
0.015830183401703835,
0.03549809381365776,
-0.03108937107026577,
-0.06238265708088875,
0.04740288481116295,
0.0895322635769844,
-0.2837618589401245,
-0.013802021741867065,
0.020817391574382782,
0.03387758880853653,
0.002302582608535886,
-0.03513839840888977,
0.04959651827812195,
-0.008404592052102089,
-0.01058711763471365,
0.040321916341781616,
0.009463046677410603,
0.08267270028591156,
0.01653508096933365,
-0.009999748319387436,
-0.014147383160889149,
0.005926384590566158,
0.05133078992366791,
-0.0027627693489193916,
0.004883556626737118,
-0.024384209886193275,
-0.021315526217222214,
-0.010214414447546005,
0.12459994852542877,
-0.005611499771475792,
0.0009335119975730777,
0.08279481530189514,
0.01702165976166725,
0.0425538569688797,
-0.026334358379244804,
-0.06979405879974365,
-0.06675878167152405,
0.05059615522623062,
0.0705769807100296,
-0.028146902099251747,
0.051444560289382935,
0.03404974937438965,
-0.03144320845603943,
0.004314964637160301,
0.034728653728961945,
0.0012880933936685324,
0.010861302725970745,
-0.0032157953828573227,
-0.05508643761277199,
-0.024682676419615746,
0.05076843872666359,
-0.0471329502761364,
-0.05108276754617691,
-0.08127713948488235,
-0.024253301322460175,
0.0011704779462888837,
-0.052522189915180206,
-0.03037053532898426,
0.011342275887727737,
0.07383176684379578,
0.04333127290010452,
0.004527188837528229,
-0.05524564906954765,
-0.06719601899385452,
0.05915003642439842,
-0.04676884040236473,
-0.015427769161760807,
0.03922370821237564,
-0.021142490208148956,
0.041259318590164185,
-0.007652768865227699
] |
PMID:25154 | Plasma level monitoring of antipsychotic drugs. | Psychotic patients treated with identical doses of antipsychotic drugs have been shown to have great interindividual differences in their steady state plasma concentration. Therefore, monitoring treatment by dosage adjustment alone is of little value. If antipsychotic blood levels can be related to clinical response then their routine measurement may well result in well defined guidelines to individualised optimal dosage. Despite the considerable effort expended in this field and the many interesting testable hypotheses generated, little substantive evidence for an acceptable plasma level monitoring guide has been reported to date. Work on metabolite level profiles, intra- and extracellular drug concentration differences, more detailed clinical rating scales, and improved experimental design, all show great promise for the future. Investigation of the pharmacokinetics and the elucidation of the often complex metabolic pathways of individual antipsychotic drugs are generating the data base required for the rational pharmacotherapy of these most severely ill patients. Until more data are available, routine monitoring of antipsychotic drug plasma levels remains of research interest. | Plasma level monitoring of antipsychotic drugs. Psychotic patients treated with identical doses of antipsychotic drugs have been shown to have great interindividual differences in their steady state plasma concentration. Therefore, monitoring treatment by dosage adjustment alone is of little value. If antipsychotic blood levels can be related to clinical response then their routine measurement may well result in well defined guidelines to individualised optimal dosage. Despite the considerable effort expended in this field and the many interesting testable hypotheses generated, little substantive evidence for an acceptable plasma level monitoring guide has been reported to date. Work on metabolite level profiles, intra- and extracellular drug concentration differences, more detailed clinical rating scales, and improved experimental design, all show great promise for the future. Investigation of the pharmacokinetics and the elucidation of the often complex metabolic pathways of individual antipsychotic drugs are generating the data base required for the rational pharmacotherapy of these most severely ill patients. Until more data are available, routine monitoring of antipsychotic drug plasma levels remains of research interest. | [
-0.06276851147413254,
0.026653461158275604,
0.020251868292689323,
0.00486797234043479,
-0.019678710028529167,
-0.02290894277393818,
0.04625818505883217,
0.04419247433543205,
-0.03693867474794388,
-0.04378234222531319,
0.050570011138916016,
-0.03976051136851311,
-0.027527712285518646,
0.08078773319721222,
-0.037194084376096725,
0.0032188203185796738,
0.060126837342977524,
-0.04613548517227173,
-0.02020975761115551,
-0.003968121949583292,
-0.01468068826943636,
-0.04188435897231102,
0.025459086522459984,
0.08134866505861282,
0.04153000935912132,
0.035087160766124725,
0.001562714111059904,
-0.059700969606637955,
-0.08608321100473404,
-0.17167608439922333,
0.06040692329406738,
-0.01480751670897007,
0.008378888480365276,
-0.024284664541482925,
-0.020968450233340263,
0.0052648670971393585,
0.004443001933395863,
0.09413894265890121,
0.036917880177497864,
0.025273649021983147,
0.011459401808679104,
0.026522647589445114,
-0.015284252353012562,
0.05653733015060425,
-0.01874024048447609,
-0.052345968782901764,
-0.041440121829509735,
0.003323738230392337,
0.05261196196079254,
0.029542218893766403,
-0.0027663251385092735,
0.0250171460211277,
0.02412014827132225,
0.028375353664159775,
-0.04673611745238304,
-0.00866642128676176,
0.004020344465970993,
0.019857201725244522,
0.02599027007818222,
0.060832954943180084,
-0.05158672854304314,
0.054967693984508514,
-0.1289406716823578,
0.004763612058013678,
0.030230678617954254,
0.017881248146295547,
-0.05273374170064926,
-0.053404878824949265,
0.009663526900112629,
0.0390181690454483,
-0.06877259910106659,
-0.03306885063648224,
-0.02990800328552723,
0.017370548099279404,
0.035158831626176834,
-0.04693603515625,
0.022060038521885872,
-0.02519034966826439,
-0.035076696425676346,
-0.041814252734184265,
0.001085879048332572,
-0.04949711635708809,
0.015369909815490246,
-0.032637014985084534,
0.03415178507566452,
-0.05402923375368118,
0.06466780602931976,
0.020603183656930923,
0.03196388855576515,
-0.00367047730833292,
-0.0207857433706522,
-0.022202719002962112,
-0.018639110028743744,
0.0036752300802618265,
-0.020978592336177826,
-0.010388888418674469,
-0.0037763009313493967,
-0.03338851034641266,
-0.003531756578013301,
0.39359113574028015,
0.016279831528663635,
-0.02142292447388172,
-0.07350773364305496,
0.008659490384161472,
-0.0351647324860096,
-0.024115029722452164,
0.03610549494624138,
0.011605992913246155,
0.02983717806637287,
-0.02110915072262287,
0.03403418883681297,
0.022267645224928856,
0.07050010561943054,
-0.06911215931177139,
0.000869575422257185,
0.0479428693652153,
0.010200779885053635,
0.013649324886500835,
0.013555155135691166,
0.03410106152296066,
-0.018428953364491463,
-0.01979460008442402,
0.029442783445119858,
-0.054147038608789444,
0.07731036841869354,
0.026877574622631073,
-0.0033341634552925825,
0.05171925947070122,
0.03295455500483513,
-0.008396942168474197,
0.06196289509534836,
0.013248845934867859,
-0.03574177995324135,
-0.001955654239282012,
0.006746335886418819,
0.019889727234840393,
0.04732007160782814,
0.0164802186191082,
0.13172641396522522,
0.06613573431968689,
-0.006200061645358801,
-0.01792861521244049,
-0.021813711151480675,
-0.08493278175592422,
-0.047595251351594925,
0.12211492657661438,
0.00006619098712690175,
-0.012261774390935898,
-0.013253987766802311,
-0.03599582985043526,
-0.04132784903049469,
0.048146165907382965,
-0.033083945512771606,
0.006701677106320858,
-0.04925151541829109,
0.027933809906244278,
-0.031192809343338013,
-0.01483841985464096,
-0.05035898834466934,
0.018434977158904076,
-0.067586250603199,
-0.05952923372387886,
-0.04138575866818428,
0.14755992591381073,
0.001053077052347362,
0.015952996909618378,
-0.005765942856669426,
0.003336466383188963,
-0.056263938546180725,
0.02535060979425907,
0.006667039357125759,
-0.021088849753141403,
-0.02856745943427086,
0.0001819573517423123,
-0.050657130777835846,
-0.07249344885349274,
0.0007955675246194005,
0.03204600140452385,
-0.028819041326642036,
-0.02713913656771183,
0.06235308572649956,
0.03150615096092224,
-0.008326767012476921,
0.017251180484890938,
0.019813966006040573,
-0.03229990229010582,
-0.02961200848221779,
0.02832975797355175,
0.03842396289110184,
0.07139008492231369,
-0.03862569108605385,
-0.018202122300863266,
-0.04602061212062836,
0.05204866826534271,
-0.04970230162143707,
-0.021256890147924423,
-0.07851991057395935,
0.06510930508375168,
-0.062112413346767426,
0.010316254571080208,
0.06961345672607422,
0.058150406926870346,
0.0018551514949649572,
0.07772596925497055,
0.05137249082326889,
0.044596560299396515,
-0.020034508779644966,
0.027111101895570755,
0.06377265602350235,
0.014392836950719357,
0.004288907628506422,
-0.009056746028363705,
-0.014243834652006626,
0.04790780320763588,
-0.08796998113393784,
0.012412101030349731,
-0.016888920217752457,
0.006672868505120277,
0.04111567512154579,
0.026169011369347572,
-0.01885104551911354,
0.016689777374267578,
-0.03693122789263725,
-0.2986616790294647,
-0.012966621667146683,
0.016514163464307785,
0.04088248312473297,
0.03096434287726879,
-0.019955957308411598,
0.012952850200235844,
-0.026451537385582924,
-0.02452937327325344,
-0.007869443856179714,
0.056294746696949005,
0.05024261772632599,
-0.02869327925145626,
-0.07563208788633347,
-0.01640983484685421,
0.017555586993694305,
-0.002501201117411256,
-0.00866231694817543,
-0.0171150304377079,
-0.04992787167429924,
-0.023984607309103012,
-0.002120832446962595,
-0.0008965540328063071,
0.0035274731926620007,
0.05608595907688141,
0.021259479224681854,
0.10191941261291504,
-0.046359430998563766,
-0.02003813162446022,
-0.0022780911531299353,
-0.015618778765201569,
0.0006206946563906968,
-0.01839311048388481,
-0.10719088464975357,
-0.03177681937813759,
-0.06855697184801102,
-0.019360627979040146,
-0.02161433920264244,
-0.0199937354773283,
0.01428128220140934,
-0.05046284943819046,
0.021864792332053185,
0.0450025238096714,
-0.06806579977273941,
-0.1438230574131012,
0.014710093848407269,
-0.0031603306997567415,
0.04262900725007057,
-0.020534776151180267,
-0.02913520485162735,
-0.028956983238458633,
0.006959265563637018,
0.01587502844631672,
-0.08773966878652573,
0.07670328766107559,
0.0136416619643569,
-0.1038917601108551,
-0.03941086307168007,
-0.09253662079572678,
-0.007737286854535341,
0.026955613866448402,
0.021370798349380493,
0.020186832174658775,
0.004555264487862587,
0.0035356637090444565,
0.006546177435666323,
0.0275079607963562,
-0.020898539572954178,
0.06782948225736618,
0.026999248191714287,
0.03199834004044533,
0.09542428702116013,
-0.06898616999387741,
0.06590623408555984,
0.0950445756316185,
0.04149625822901726,
-0.04480764642357826,
0.007686065975576639,
-0.024894706904888153,
0.06316734105348587,
0.04070457071065903,
-0.04026186838746071,
0.020458001643419266,
0.03747578710317612,
-0.0020643891766667366,
0.04401873052120209,
-0.011213860474526882,
0.015104326419532299,
0.03605608642101288,
0.010372299700975418,
-0.023275474086403847,
0.01155882515013218,
0.0038768104277551174,
-0.10096213966608047,
-0.008953224867582321,
0.07633627951145172,
-0.22102560102939606,
0.034091394394636154,
0.006675657816231251,
0.013810708187520504,
0.03935582563281059,
0.01703830435872078,
0.04957142099738121,
0.04396066069602966,
-0.02169356308877468,
-0.03541923686861992,
0.031806230545043945,
0.010026085190474987,
0.047243569046258926,
0.06413757055997849,
-0.03148605301976204,
-0.0011215321719646454,
0.060385219752788544,
-0.025933265686035156,
0.0013877673773095012,
0.011468607932329178,
0.018782172352075577,
0.0013905030209571123,
0.12385059893131256,
0.02180130034685135,
-0.008754896931350231,
0.017297502607107162,
0.031894925981760025,
0.005494242999702692,
-0.025766415521502495,
-0.0033043951261788607,
-0.08354493230581284,
0.02549603395164013,
0.056271154433488846,
-0.016584690660238266,
0.02551995776593685,
0.007460522465407848,
-0.009386593475937843,
-0.024277765303850174,
0.03158669173717499,
0.0015856458339840174,
-0.028959907591342926,
-0.00009260224760510027,
-0.029455434530973434,
-0.002198147354647517,
0.06528875976800919,
-0.036821868270635605,
-0.032212723046541214,
-0.06652584671974182,
-0.05190445855259895,
0.00172385701444,
-0.027177440002560616,
-0.039125531911849976,
-0.009551296941936016,
0.060378547757864,
0.03098410740494728,
0.025424635037779808,
-0.023145293816924095,
-0.05363449454307556,
0.0246303528547287,
-0.0496225506067276,
-0.03788720816373825,
0.05535542592406273,
-0.017854338511824608,
0.019560273736715317,
-0.021201781928539276
] |
PMID:25156 | Diseases and drug protein binding. | In a number of pathological states a decrease in the plasma protein binding of drugs is observed. This may be due to many factors related either to the protein, or the ligand (drug), or to the binding conditions. The most important of these disease states quantitatively are probably hypoalbuminaemia, conditions resulting in modification of the albumin compartment volume and the presence on albumin binding sites of pathological inhibitors of drug binding. A decrease in the extent of drug plasma protein binding does not necessarily lead to enhanced drug effects and therefore raises two important therapeutic questions. Firstly, does reduced protein binding have a clinically significant influence on the pharmacological effects of the drug? Secondly, if it does, is it preferable to modify the dosage regimen of the drug or to correct the plasma protein concentration prior to the administration of the drug? At present, only tentative answers can be given. | Diseases and drug protein binding. In a number of pathological states a decrease in the plasma protein binding of drugs is observed. This may be due to many factors related either to the protein, or the ligand (drug), or to the binding conditions. The most important of these disease states quantitatively are probably hypoalbuminaemia, conditions resulting in modification of the albumin compartment volume and the presence on albumin binding sites of pathological inhibitors of drug binding. A decrease in the extent of drug plasma protein binding does not necessarily lead to enhanced drug effects and therefore raises two important therapeutic questions. Firstly, does reduced protein binding have a clinically significant influence on the pharmacological effects of the drug? Secondly, if it does, is it preferable to modify the dosage regimen of the drug or to correct the plasma protein concentration prior to the administration of the drug? At present, only tentative answers can be given. | [
-0.04752146080136299,
-0.0031777601689100266,
0.08021748065948486,
-0.013244467787444592,
-0.030160285532474518,
-0.010844345204532146,
0.11092502623796463,
0.03485730290412903,
0.001761999330483377,
0.008932139724493027,
-0.00919637642800808,
-0.012738758698105812,
0.015232225880026817,
0.061180539429187775,
-0.01896435022354126,
-0.05583042651414871,
0.03613291308283806,
-0.01348851528018713,
-0.09437692165374756,
0.009378126822412014,
-0.05511150881648064,
0.04776811972260475,
0.04250829294323921,
0.00914322305470705,
0.06323104351758957,
0.015465839765965939,
-0.016992023214697838,
-0.004375495947897434,
-0.06908901780843735,
-0.15606635808944702,
-0.004210201092064381,
0.020283278077840805,
-0.007964848540723324,
-0.004241413902491331,
-0.026700997725129128,
0.007672385778278112,
-0.02983008325099945,
0.02583221159875393,
-0.019619092345237732,
0.022196820005774498,
0.07002697139978409,
0.05277295410633087,
-0.016635488718748093,
0.00578628946095705,
0.020456530153751373,
-0.0024802451953291893,
-0.03300432115793228,
0.048739004880189896,
0.04864627495408058,
0.026240181177854538,
0.00807485543191433,
-0.02449801191687584,
-0.003448916831985116,
0.09806984663009644,
0.027442587539553642,
0.020700406283140182,
-0.03261217102408409,
0.10591568052768707,
0.005368716083467007,
0.07804489880800247,
-0.012254343368113041,
0.03653367608785629,
-0.08181412518024445,
0.0692351907491684,
0.09507028013467789,
-0.008257868699729443,
-0.021736372262239456,
-0.03958369418978691,
0.042250119149684906,
0.06953929364681244,
-0.04236671328544617,
0.03313644602894783,
-0.04036989063024521,
0.050733476877212524,
-0.022220343351364136,
-0.08057167381048203,
-0.02998976781964302,
0.023110345005989075,
0.04102788493037224,
-0.02947036176919937,
0.005660714115947485,
-0.031101031228899956,
0.037683021277189255,
-0.012735276482999325,
-0.01636328548192978,
-0.017211180180311203,
0.010180938988924026,
-0.05658739432692528,
-0.011392452754080296,
-0.03773708641529083,
-0.030150623992085457,
-0.07338309288024902,
0.022142663598060608,
0.0449385792016983,
-0.05764562264084816,
0.025736985728144646,
-0.03213144466280937,
-0.02517586573958397,
-0.07290127128362656,
0.32906273007392883,
-0.051090363413095474,
-0.005982537288218737,
-0.08207757771015167,
0.051471978425979614,
-0.027450762689113617,
-0.012230581603944302,
0.014814631082117558,
-0.050386395305395126,
0.0016298409318551421,
0.0405215360224247,
0.03704959899187088,
0.03044542856514454,
0.025177927687764168,
-0.010089369490742683,
0.008609974756836891,
-0.0002160870935767889,
0.005627326667308807,
0.08051839470863342,
-0.05524182319641113,
0.08673284202814102,
-0.0318441316485405,
0.04359355941414833,
0.04131357744336128,
-0.0029394677840173244,
0.09453225880861282,
0.03493384271860123,
-0.004516178276389837,
0.09032345563173294,
0.0054354071617126465,
-0.0013598381774500012,
0.03734477981925011,
0.0040814741514623165,
-0.04207966476678848,
-0.012066391296684742,
0.03456516191363335,
-0.02566906064748764,
-0.002358997706323862,
-0.04558442905545235,
0.08503296971321106,
0.06165093928575516,
-0.02664870396256447,
-0.023687470704317093,
-0.07829762995243073,
-0.05006449669599533,
-0.041280318051576614,
0.127780944108963,
-0.024534061551094055,
-0.014535478316247463,
-0.013983827084302902,
-0.006241216789931059,
-0.013215882703661919,
0.03719198703765869,
-0.03272823616862297,
0.08379123359918594,
-0.05186556279659271,
0.038673873990774155,
0.03810005635023117,
-0.019452989101409912,
-0.02085776999592781,
0.010020856745541096,
-0.08883380144834518,
-0.02066214755177498,
-0.016829680651426315,
0.030195094645023346,
-0.04182231053709984,
0.042841777205467224,
-0.048419490456581116,
0.018017254769802094,
-0.012316998094320297,
0.02384771592915058,
-0.06015283614397049,
0.012589829973876476,
0.016760479658842087,
0.017211899161338806,
-0.005921230651438236,
-0.0017875791527330875,
0.0007404693751595914,
0.027013706043362617,
-0.06503436714410782,
-0.07675040513277054,
0.030958332121372223,
0.05576227232813835,
0.010166291147470474,
-0.003648236161097884,
0.009216448292136192,
-0.014884761534631252,
-0.04298887774348259,
0.09806852042675018,
0.0687049925327301,
0.071823850274086,
-0.0066367099061608315,
0.03281179443001747,
-0.06765380501747131,
-0.0028185786213725805,
-0.07613883912563324,
0.0006401815917342901,
-0.04196516424417496,
0.028473978862166405,
-0.041778646409511566,
0.007445208262652159,
0.10471545159816742,
0.03883018717169762,
-0.015112760476768017,
0.053760040551424026,
0.027040548622608185,
0.07494188845157623,
0.03076750598847866,
-0.03820238634943962,
0.0350959487259388,
-0.0427103117108345,
-0.027525100857019424,
-0.009772892110049725,
-0.07254434376955032,
-0.014291816391050816,
-0.021810656413435936,
0.019998980686068535,
0.0021073040552437305,
0.00020758147002197802,
0.009330039843916893,
-0.026993952691555023,
-0.0038352031260728836,
0.005422194488346577,
0.01004539430141449,
-0.31717759370803833,
0.018569983541965485,
-0.01341250166296959,
-0.010310429148375988,
-0.01729726418852806,
-0.03010842576622963,
-0.06358922272920609,
0.05568249523639679,
-0.03483184054493904,
-0.007371679879724979,
0.03437625989317894,
0.0437779538333416,
-0.009291289374232292,
-0.08584779500961304,
-0.04252072423696518,
-0.011223677545785904,
0.02600652351975441,
-0.07631539553403854,
-0.026056932285428047,
-0.026807613670825958,
-0.0610802061855793,
-0.008707216940820217,
0.010517583228647709,
0.017856627702713013,
0.06005784869194031,
-0.009223484434187412,
0.07175250351428986,
-0.027671057730913162,
-0.09204768389463425,
-0.01429190393537283,
0.01584516279399395,
0.029158728197216988,
-0.06246563047170639,
-0.08036769926548004,
-0.020363852381706238,
-0.016239751130342484,
-0.02741410583257675,
-0.013891438953578472,
-0.03606401011347771,
0.03271271660923958,
-0.05827324837446213,
0.062150049954652786,
0.01538783498108387,
0.015785161405801773,
-0.07645177096128464,
0.038792312145233154,
0.022668398916721344,
0.011684831231832504,
-0.015898579731583595,
0.05618289113044739,
0.04780185967683792,
0.0187129657715559,
-0.011093915440142155,
0.02115922048687935,
0.004779586102813482,
0.010036694817245007,
-0.02454148232936859,
0.04458776116371155,
-0.04227186366915703,
0.0405854694545269,
0.023517003282904625,
-0.02367270365357399,
0.01715526357293129,
-0.044773221015930176,
-0.02205261029303074,
-0.005859494674950838,
0.00007986705168150365,
-0.052310761064291,
0.02857086807489395,
0.05519572272896767,
-0.027096064761281013,
0.10863670706748962,
-0.07408063113689423,
0.10132775455713272,
0.12040135264396667,
0.017230071127414703,
-0.028523273766040802,
0.016015756875276566,
-0.054183389991521835,
0.027279362082481384,
0.0043066819198429585,
-0.020416168496012688,
0.015005773864686489,
0.0031446407083421946,
0.010221434757113457,
0.0023021979723125696,
0.04795445129275322,
-0.011211850680410862,
-0.04744276404380798,
-0.0003993071150034666,
-0.03487261012196541,
-0.023734645918011665,
-0.06941497325897217,
-0.008474714122712612,
-0.025386858731508255,
0.04068506881594658,
-0.2370893508195877,
0.06739982217550278,
0.07476107031106949,
0.03374557942152023,
-0.032070279121398926,
0.06649626791477203,
0.046431977301836014,
0.0004629947943612933,
-0.05762400105595589,
-0.03195848688483238,
0.02775580994784832,
0.006998671218752861,
0.1054186224937439,
-0.03890085592865944,
-0.05835021659731865,
-0.02108168601989746,
0.057384513318538666,
-0.039510034024715424,
0.017039399594068527,
-0.00029966962756589055,
0.010567699559032917,
0.0136113241314888,
0.13809730112552643,
-0.058218833059072495,
0.050302859395742416,
0.020281415432691574,
-0.015604977495968342,
0.05365553870797157,
-0.07961821556091309,
0.0002784529060591012,
-0.03723451867699623,
-0.0002741050557233393,
0.08041707426309586,
-0.003976502921432257,
-0.044678956270217896,
0.016075516119599342,
-0.030636446550488472,
0.02372901514172554,
-0.017771057784557343,
0.04394330829381943,
-0.027781039476394653,
-0.03091290220618248,
-0.08218810707330704,
0.00027683854568749666,
0.05571147799491882,
0.0002343532833037898,
-0.019251542165875435,
-0.02758028544485569,
0.02453453280031681,
0.008353129029273987,
-0.023406362161040306,
-0.0030799969099462032,
-0.027998948469758034,
0.03643805906176567,
0.015579168684780598,
-0.005190965719521046,
-0.0036306465044617653,
-0.006724519655108452,
0.01609763875603676,
-0.005628400016576052,
-0.012616082094609737,
-0.007351270876824856,
-0.10982558876276016,
0.012992789968848228,
-0.021573945879936218
] |
PMID:25163 | Acid-base balance in small premature infants (less than 1500 grams) with type II RDS. | Twenty-one small premature infants with clinical features and radiological findings of type II RDS were studied. Correction of blood pH, corresponding with the decrease of blood PaCO2, was usually seen in the first eight hours of age. Clinical inprovement, as judged by the RDS score, was noted only after 12 hours of age. Complete recovery was the rule although signs of respiratory distress in a few infants lasted for 48-72 hours. The calculated AaDO2 and total Qs/Qt weer slightly higher (27.8%) than reported in normal infants and remained unchanged until after 48 hours of age when it decreased to 24.5%. | Acid-base balance in small premature infants (less than 1500 grams) with type II RDS. Twenty-one small premature infants with clinical features and radiological findings of type II RDS were studied. Correction of blood pH, corresponding with the decrease of blood PaCO2, was usually seen in the first eight hours of age. Clinical inprovement, as judged by the RDS score, was noted only after 12 hours of age. Complete recovery was the rule although signs of respiratory distress in a few infants lasted for 48-72 hours. The calculated AaDO2 and total Qs/Qt weer slightly higher (27.8%) than reported in normal infants and remained unchanged until after 48 hours of age when it decreased to 24.5%. | [
-0.07431807368993759,
0.005655132699757814,
-0.03447825461626053,
0.02781377173960209,
0.007002625148743391,
0.025103585794568062,
0.004976939409971237,
0.013884056359529495,
-0.0330074168741703,
0.03200218454003334,
0.019095508381724358,
-0.06037699431180954,
0.02550656907260418,
0.04826826602220535,
-0.006674931384623051,
-0.03843154385685921,
0.03446128964424133,
-0.0199770238250494,
-0.047818880528211594,
0.058314092457294464,
-0.009365692734718323,
0.03621549904346466,
0.009250142611563206,
-0.019595813006162643,
-0.004636053927242756,
0.043450966477394104,
-0.0024912357330322266,
-0.014936352148652077,
-0.030812740325927734,
-0.20769132673740387,
0.06582201272249222,
-0.02834545075893402,
0.004038381390273571,
-0.006064071319997311,
-0.02033708430826664,
0.03588515520095825,
0.0305187851190567,
0.04673464968800545,
0.0015094655100256205,
0.0202315840870142,
0.01990361139178276,
0.009090122766792774,
0.035461004823446274,
0.010883116163313389,
-0.03067794255912304,
-0.006063172593712807,
-0.06391916424036026,
0.02443745732307434,
0.11192650347948074,
0.02437984198331833,
-0.020713917911052704,
0.03583819419145584,
0.05833546072244644,
0.08119705319404602,
0.01215416006743908,
-0.013093873858451843,
0.04165857285261154,
0.004836373031139374,
0.0572352334856987,
-0.00476417038589716,
0.040871139615774155,
0.03469039872288704,
-0.16036182641983032,
0.02537197805941105,
0.013492077589035034,
0.06551238894462585,
-0.05011719465255737,
-0.0788750872015953,
-0.006438549142330885,
0.02629246562719345,
-0.022685488685965538,
0.06249260529875755,
-0.017112664878368378,
0.012410392984747887,
-0.029649334028363228,
0.009179238229990005,
-0.0065404088236391544,
-0.06784822046756744,
0.07854223251342773,
0.0016960763605311513,
-0.05294649675488472,
0.029656199738383293,
-0.05033821985125542,
0.03387828543782234,
0.007669486571103334,
-0.0106901153922081,
-0.0051711625419557095,
-0.053812991827726364,
-0.043916407972574234,
0.05426397919654846,
-0.009926228784024715,
-0.06243819743394852,
-0.040344882756471634,
0.05962454527616501,
-0.03137137368321419,
-0.006246988195925951,
0.01884729601442814,
-0.038077786564826965,
0.00957793090492487,
0.38656318187713623,
0.003470230381935835,
0.027544280514121056,
-0.03621164709329605,
-0.016390785574913025,
0.022051017731428146,
-0.03943390026688576,
0.02089031971991062,
-0.016516070812940598,
-0.0163729228079319,
0.019314086064696312,
-0.017925282940268517,
0.044704336673021317,
0.05591881647706032,
-0.09079049527645111,
-0.004556091036647558,
0.02988075092434883,
0.05235636979341507,
0.08777005970478058,
0.03550756350159645,
0.046540066599845886,
-0.014731056988239288,
0.0009412684012204409,
0.039446115493774414,
-0.01788771152496338,
0.07815752923488617,
0.0016473246505483985,
0.0672837346792221,
0.060720302164554596,
0.0007989765726961195,
0.05846576765179634,
0.0077092950232326984,
-0.0009775743819773197,
-0.04567423835396767,
-0.021478448063135147,
-0.010640681721270084,
-0.02072802372276783,
0.04332330450415611,
-0.004668016918003559,
0.05296199396252632,
-0.020122388377785683,
-0.016013365238904953,
-0.06804269552230835,
-0.04032281041145325,
-0.04039532318711281,
-0.05419446527957916,
0.07811073958873749,
-0.05029365420341492,
0.07915344089269638,
-0.0011032355250790715,
-0.0132956113666296,
0.01042413990944624,
0.06852604448795319,
-0.009558049030601978,
-0.01685621216893196,
0.014974535442888737,
-0.014958411455154419,
-0.03961644694209099,
-0.0129238897934556,
-0.0014436820056289434,
-0.05580393597483635,
-0.031361404806375504,
-0.07802774757146835,
-0.004628906492143869,
0.015366971492767334,
-0.02028246410191059,
-0.005726587492972612,
0.004339628852903843,
-0.01816723309457302,
0.05476119741797447,
-0.02150457724928856,
0.01945863850414753,
0.004924721084535122,
0.06182887405157089,
-0.08960415422916412,
0.043690524995326996,
-0.008038650266826153,
0.0025378356222063303,
-0.0028929817490279675,
0.033050935715436935,
-0.002077010227367282,
0.014853016473352909,
0.0009716710774227977,
-0.007757715880870819,
-0.02214132808148861,
0.01749192550778389,
-0.007926168851554394,
-0.06504826247692108,
0.03322364389896393,
0.06408271193504333,
0.017841225489974022,
-0.0030879511032253504,
-0.06349723786115646,
-0.10925212502479553,
0.012333200313150883,
0.043597787618637085,
0.02573174051940441,
-0.05241912975907326,
-0.0073282537050545216,
-0.014981822110712528,
0.025505414232611656,
0.054764192551374435,
-0.015552109107375145,
0.03905981406569481,
0.009864475578069687,
-0.004696278367191553,
0.025054557248950005,
0.05985075235366821,
-0.0029684642795473337,
-0.004283655900508165,
0.037614595144987106,
0.012200631201267242,
0.0294156726449728,
-0.07587020844221115,
-0.013453311286866665,
-0.065485879778862,
0.08720645308494568,
-0.005922717973589897,
-0.02187439613044262,
-0.031013498082756996,
-0.007953977212309837,
0.003672714112326503,
-0.02485273778438568,
-0.0025758203119039536,
-0.30467304587364197,
0.008344277739524841,
0.03262826055288315,
-0.029517369344830513,
0.07483848929405212,
-0.04991782829165459,
-0.029125256463885307,
-0.014526022598147392,
0.012910963967442513,
-0.049294810742139816,
0.011231396347284317,
0.07105643302202225,
-0.04220740124583244,
0.00010657620441634208,
-0.05566658824682236,
0.029425524175167084,
0.03723922744393349,
-0.04219060763716698,
0.031779441982507706,
-0.01249945443123579,
0.0424501933157444,
0.022114375606179237,
0.0024761680979281664,
0.020869657397270203,
0.0941801369190216,
0.010251949541270733,
0.12352839857339859,
-0.09778788685798645,
-0.017725655809044838,
-0.02534306049346924,
0.04541231691837311,
-0.0107408557087183,
-0.008365090936422348,
-0.02304629608988762,
-0.0011024444829672575,
-0.0339629091322422,
-0.05185060203075409,
-0.0003148427349515259,
-0.059670716524124146,
0.007714991923421621,
-0.0460897758603096,
0.03363407403230667,
-0.021755989640951157,
-0.051975492388010025,
-0.0569092221558094,
0.01187878381460905,
-0.03237820044159889,
-0.017724880948662758,
-0.03890904784202576,
-0.012757308781147003,
-0.0017887086141854525,
-0.0010373315308243036,
-0.05902872234582901,
-0.05288003757596016,
0.02817228063941002,
-0.004073086194694042,
-0.0313011072576046,
-0.01639706827700138,
-0.07297638058662415,
-0.03815316781401634,
-0.06091998890042305,
-0.0021955298725515604,
-0.004976795520633459,
-0.018847746774554253,
-0.029141535982489586,
-0.033258192241191864,
0.04789107292890549,
0.005595542956143618,
0.03514093533158302,
0.00584899727255106,
0.010790764354169369,
0.07890960574150085,
-0.10282241553068161,
-0.01090250164270401,
0.03294377774000168,
-0.004631801974028349,
-0.061341073364019394,
-0.03410542011260986,
-0.005268941167742014,
-0.0000011104636996606132,
0.019779425114393234,
-0.05546537786722183,
0.048863064497709274,
0.056891169399023056,
0.012846289202570915,
0.03981693461537361,
0.053768306970596313,
-0.020262697711586952,
-0.01568129099905491,
0.015459761954843998,
0.020659424364566803,
-0.020265687257051468,
-0.010954910889267921,
0.03238973021507263,
0.015092629007995129,
0.030725684016942978,
-0.2924596965312958,
0.0036980060394853354,
-0.020292144268751144,
0.00043559697223827243,
-0.02186758629977703,
0.02000775933265686,
-0.020013462752103806,
-0.0056781587190926075,
0.0063155763782560825,
0.00029516860377043486,
0.010257200337946415,
0.01898290403187275,
0.09938176721334457,
-0.006139308214187622,
-0.042585618793964386,
0.037403374910354614,
0.005146675277501345,
-0.0341632142663002,
0.026365185156464577,
-0.04148209095001221,
0.03957842290401459,
-0.020908240228891373,
0.13012678921222687,
0.0045169941149652,
0.001249302295036614,
0.012506609782576561,
-0.053321100771427155,
0.03975493460893631,
-0.04612847417593002,
0.0387863889336586,
0.023740101605653763,
0.05124083533883095,
0.07470549643039703,
0.003436796832829714,
0.06239508464932442,
0.011220059357583523,
-0.1154344379901886,
0.029852038249373436,
0.03412291780114174,
0.03436034917831421,
0.02289581298828125,
-0.04224444925785065,
-0.03488515689969063,
-0.009231706149876118,
0.07536513358354568,
-0.059385012835264206,
0.009561273269355297,
-0.06985343247652054,
0.012451395392417908,
-0.023708397522568703,
-0.03880232945084572,
-0.04674828425049782,
0.036438096314668655,
0.008043406531214714,
0.056903399527072906,
0.008297435939311981,
-0.009806796908378601,
-0.0023949153255671263,
0.024562394246459007,
-0.04771940037608147,
0.0468970350921154,
-0.005195925943553448,
0.024222886189818382,
0.09025350958108902,
0.009974944405257702
] |
PMID:25167 | Loxapine: a review of its pharmacological properties and therapeutic efficacy as an antipsychotic agent. | Loxapine is a dibenzoxazepine, tricyclic compound recommended for the treatment of acute and chronic schizophrenia. In its therapeutic effectiveness and profile and incidence of side-effects, loxapine closely resembles the traditional antipsychotic agents. Although loxapine has tended to be less effective than some standard antipsychotic drugs in a few short-term (3 to 4 weeks) studies, it has been superior to a placebo and about as effective as chlorpromazine, haloperidol, trifluoperazine or thiothixene when evaluated after 4 to 12 weeks. Like the phenothiazine (e.g. chlorpromazine) and butyrophenone (e.g. haloperidol) antipsychotic agents, loxapine causes a high incidence of extrapyramidal reactions. Sedation occurs frequently, especially during early stages of treatment. Other, less common side-effects such as anticholinergic effects (dry mouth, blurred vision, etc.), hypotension, tachycardia and precipitation of epileptic seizures, which occur with the older antipsychotic drugs, have also been reported with loxapine. | Loxapine: a review of its pharmacological properties and therapeutic efficacy as an antipsychotic agent. Loxapine is a dibenzoxazepine, tricyclic compound recommended for the treatment of acute and chronic schizophrenia. In its therapeutic effectiveness and profile and incidence of side-effects, loxapine closely resembles the traditional antipsychotic agents. Although loxapine has tended to be less effective than some standard antipsychotic drugs in a few short-term (3 to 4 weeks) studies, it has been superior to a placebo and about as effective as chlorpromazine, haloperidol, trifluoperazine or thiothixene when evaluated after 4 to 12 weeks. Like the phenothiazine (e.g. chlorpromazine) and butyrophenone (e.g. haloperidol) antipsychotic agents, loxapine causes a high incidence of extrapyramidal reactions. Sedation occurs frequently, especially during early stages of treatment. Other, less common side-effects such as anticholinergic effects (dry mouth, blurred vision, etc.), hypotension, tachycardia and precipitation of epileptic seizures, which occur with the older antipsychotic drugs, have also been reported with loxapine. | [
-0.0423249751329422,
0.03383127599954605,
0.004918846767395735,
0.06012194603681564,
0.008021755144000053,
-0.020268956199288368,
0.05097239091992378,
0.05175589397549629,
-0.03966036066412926,
-0.04630114510655403,
0.049828119575977325,
-0.03176247701048851,
0.024773549288511276,
0.030521772801876068,
0.013770374469459057,
-0.03371651843190193,
0.013406822457909584,
-0.0411377027630806,
-0.016047049313783646,
-0.061088547110557556,
-0.03157208487391472,
0.019964177161455154,
0.0205213762819767,
0.07724873721599579,
0.029796425253152847,
-0.01719478704035282,
-0.03601163625717163,
0.022771578282117844,
-0.027661548927426338,
-0.11815601587295532,
0.02119956724345684,
0.04237048700451851,
0.03841738402843475,
-0.050442371517419815,
-0.037846192717552185,
0.013701748102903366,
-0.03954942524433136,
0.09569627791643143,
-0.044960785657167435,
-0.008121496066451073,
0.06614813953638077,
0.10410226881504059,
0.026653515174984932,
-0.027134984731674194,
0.059411369264125824,
-0.07831187546253204,
-0.019166022539138794,
-0.04214243218302727,
0.05898810923099518,
-0.006899080239236355,
0.04191646724939346,
-0.016025682911276817,
0.0698491558432579,
0.02382460981607437,
-0.018791714683175087,
0.006251719314604998,
-0.015267528593540192,
0.024217931553721428,
-0.09971105307340622,
0.07401727139949799,
-0.005079963244497776,
0.01724093221127987,
-0.09656734019517899,
0.037626925855875015,
-0.02403516136109829,
0.0006990300607867539,
-0.012714073061943054,
-0.07638749480247498,
0.01016368716955185,
0.05007197707891464,
-0.0756634920835495,
-0.03520645946264267,
0.006445057690143585,
0.022009970620274544,
0.03355791047215462,
-0.027523575350642204,
0.00016986505943350494,
-0.029031436890363693,
-0.06619037687778473,
-0.05012365058064461,
-0.0005033071502111852,
-0.08353856950998306,
0.011136804707348347,
-0.08777699619531631,
0.052029114216566086,
0.007382434327155352,
0.03104424849152565,
0.015868179500102997,
0.008708557114005089,
-0.03130549192428589,
0.023254668340086937,
-0.0034876482095569372,
0.009058874100446701,
-0.03869279846549034,
0.008622774854302406,
0.044289473444223404,
-0.02150985598564148,
-0.042786549776792526,
-0.014070951379835606,
0.36427944898605347,
0.000517838925588876,
0.04466430842876434,
-0.04470057785511017,
-0.04191335290670395,
0.017980875447392464,
-0.033118125051259995,
0.06289772689342499,
-0.028332099318504333,
0.010218552313745022,
0.06874468922615051,
0.03719083592295647,
-0.006787768565118313,
0.03649032860994339,
-0.005033922381699085,
-0.06445155292749405,
-0.025020936504006386,
0.05803840979933739,
-0.03382229059934616,
0.038298361003398895,
0.10980959981679916,
-0.03356326371431351,
0.008276729844510555,
-0.02513877861201763,
-0.023455506190657616,
0.044506609439849854,
0.000823486247099936,
-0.013094047084450722,
0.053505994379520416,
0.012552372179925442,
0.0157549399882555,
0.02755698189139366,
0.0030043646693229675,
0.009304904378950596,
0.027851464226841927,
0.014502117410302162,
0.023495204746723175,
0.013894326984882355,
0.0066110603511333466,
0.07957427948713303,
0.0023383309599012136,
0.006226011551916599,
-0.035802874714136124,
0.03789949044585228,
-0.04268977791070938,
-0.041654493659734726,
0.08321656286716461,
0.009359413757920265,
0.011868822388350964,
-0.05437510460615158,
0.06382258981466293,
-0.08726503700017929,
0.017985230311751366,
0.022078657522797585,
0.04049015790224075,
-0.04553300142288208,
-0.0005934208747930825,
-0.030929595232009888,
0.011851745657622814,
-0.06680019944906235,
0.040473926812410355,
-0.031017931178212166,
0.004448117688298225,
0.02518421597778797,
0.08687550574541092,
-0.013353446498513222,
0.025169841945171356,
0.007101110648363829,
-0.006353386677801609,
-0.08831492066383362,
0.02394569292664528,
0.011481426656246185,
0.005334074608981609,
-0.006735924165695906,
-0.025297140702605247,
-0.05926945060491562,
-0.0624072402715683,
0.021500401198863983,
-0.027779165655374527,
0.05202176794409752,
-0.002911664778366685,
0.05868248641490936,
0.04178355634212494,
-0.038852572441101074,
-0.01893416978418827,
0.00016160811355803162,
0.01722259819507599,
-0.06038783863186836,
0.009076262824237347,
0.037830859422683716,
-0.014426450245082378,
-0.04408584535121918,
0.0069871325977146626,
-0.01944843679666519,
0.054802022874355316,
-0.08403219282627106,
-0.07132358849048615,
-0.09622311592102051,
-0.04525134712457657,
-0.04156654700636864,
-0.04147215187549591,
0.10936927795410156,
0.06523702293634415,
0.037238288670778275,
0.050066061317920685,
0.006674554198980331,
0.05091718211770058,
0.007911984808743,
-0.0033510299399495125,
0.02596943825483322,
-0.05460427701473236,
-0.02213086374104023,
0.05965695530176163,
0.05218292772769928,
0.04749596491456032,
-0.020459184423089027,
0.09268877655267715,
-0.046992115676403046,
0.02156876027584076,
0.055242858827114105,
-0.003931180573999882,
0.07866057008504868,
-0.046142566949129105,
-0.00039618590380996466,
-0.27509066462516785,
0.008891046978533268,
-0.0011639324948191643,
0.0013907875400036573,
0.032503772526979446,
-0.09499648958444595,
-0.007170953322201967,
-0.038771048188209534,
-0.015995904803276062,
-0.013390935957431793,
-0.008181248791515827,
0.017667030915617943,
-0.0669192522764206,
0.008756059221923351,
-0.02491481974720955,
0.01169606763869524,
0.07726899534463882,
-0.023774052038788795,
0.006912126671522856,
0.01027934905141592,
0.003558510448783636,
-0.014408769086003304,
-0.05506649985909462,
-0.013957766816020012,
0.01128795463591814,
0.07391876727342606,
0.07198528945446014,
0.017731592059135437,
-0.013038013130426407,
-0.030292214825749397,
-0.048927828669548035,
0.011251915246248245,
-0.05432946979999542,
-0.10215794295072556,
-0.007681553717702627,
0.0039175222627818584,
0.01593545824289322,
-0.05942145362496376,
-0.01007589790970087,
-0.010383903048932552,
-0.08384247869253159,
0.05261992663145065,
-0.020807044580578804,
-0.009025496430695057,
-0.15073691308498383,
0.04853306710720062,
0.004553709179162979,
0.05124634504318237,
0.016772864386439323,
-0.028116827830672264,
-0.034396857023239136,
0.09512746334075928,
0.03054298833012581,
-0.014825667254626751,
0.06735308468341827,
-0.008118869736790657,
-0.10282660275697708,
0.018475638702511787,
-0.02113870345056057,
-0.060175325721502304,
-0.000012998227248317562,
0.02669062837958336,
0.03857363760471344,
0.03337325155735016,
-0.01769893988966942,
-0.008932456374168396,
0.02300109900534153,
-0.047494132071733475,
0.06193431094288826,
0.012049444951117039,
-0.03412264585494995,
0.1563713699579239,
-0.07952792942523956,
-0.029832826927304268,
0.09263084828853607,
0.027533767744898796,
0.04061545804142952,
-0.008672388270497322,
-0.0474492684006691,
0.03009316883981228,
-0.00674367556348443,
-0.05289221554994583,
-0.020165784284472466,
0.01769273355603218,
-0.030405955389142036,
-0.035060059279203415,
-0.0419241264462471,
-0.05350472405552864,
-0.0024209707044065,
-0.018495863303542137,
0.03149484097957611,
0.03698902577161789,
-0.014926194213330746,
-0.03621174395084381,
0.0006601498462259769,
-0.002465673256665468,
-0.25510063767433167,
-0.011000778526067734,
0.010323306545615196,
0.05583794787526131,
-0.020553920418024063,
0.00928498711436987,
0.014869342558085918,
0.015214238315820694,
-0.05255591496825218,
0.03616996109485626,
-0.025708911940455437,
-0.013397996313869953,
0.07169852405786514,
-0.016806071624159813,
-0.013110203668475151,
-0.058504387736320496,
0.07766463607549667,
-0.01012072991579771,
-0.00029765928047709167,
0.04083498567342758,
-0.022315174341201782,
-0.005606646183878183,
0.11503155529499054,
0.06097955256700516,
0.020593110471963882,
0.032391227781772614,
0.007672509644180536,
-0.015449211932718754,
-0.0060208141803741455,
0.043255291879177094,
-0.01668945513665676,
0.02760492078959942,
0.06411808729171753,
-0.016890719532966614,
-0.008331104181706905,
-0.014061110094189644,
-0.08192094415426254,
-0.029930057004094124,
0.006893621291965246,
0.024356434121727943,
0.005626805126667023,
0.050254885107278824,
0.03994828462600708,
0.06220988184213638,
0.011754201725125313,
-0.07052752375602722,
-0.030566783621907234,
-0.034513894468545914,
-0.047933463007211685,
0.03263065963983536,
-0.03259456902742386,
-0.016965743154287338,
-0.01586347632110119,
0.030332809314131737,
0.031763963401317596,
-0.020549550652503967,
-0.011948548257350922,
0.024435967206954956,
0.022987643256783485,
0.014636402949690819,
-0.043951284140348434,
-0.007838900201022625,
0.034997936338186264,
0.06427745521068573,
0.06386071443557739
] |
PMID:25170 | [Hypotensive and antihypertensive activity of a newly synthesized derivative of 2-aminotetralin]. | The report describes the hypotensive and antihypertensive activity of the compound N-(trans-3-hydroxy-1,2,3,4-tetrahydro-2-naphtyl)-N'-(3-oxo-3-phenylpropyl) piperazine dihydrochloride (P11). The work is made on normotensive cats and rats as well as on spontaneous and experimental (metacorticoid) hypertension. The studies were carried out under the conditions of acute and chronic experiment as the arteria pressure was recorded by blood and bloodless method. The compound P11 show a manifested hypotensive activity with favourable duration and therapeutic width. Its antihypertensive activity is revealed in rats with spontaneous and metacorticoid hypertension both after single and chronic applciation of the compound. The influence on the blood pressure weakens after reserpinization of the animals. The compound possesses slight ganglion-blocking action and manifested alpha and beta adreno-blocking activity: diminishes noradrenaline, adrenaline and isoprenaline responses on the arterial pressure and heart activity in anesthetised cats and rats, shifts to the right the cumulative dose-response curve of noradrenaline on vas deferens of a rat, ect. The hypotensive and antihypertensive effect of the compound P11 is discussed on the basis of its alpha and beta adrenoblocking action and its specific sedative effect. | [Hypotensive and antihypertensive activity of a newly synthesized derivative of 2-aminotetralin]. The report describes the hypotensive and antihypertensive activity of the compound N-(trans-3-hydroxy-1,2,3,4-tetrahydro-2-naphtyl)-N'-(3-oxo-3-phenylpropyl) piperazine dihydrochloride (P11). The work is made on normotensive cats and rats as well as on spontaneous and experimental (metacorticoid) hypertension. The studies were carried out under the conditions of acute and chronic experiment as the arteria pressure was recorded by blood and bloodless method. The compound P11 show a manifested hypotensive activity with favourable duration and therapeutic width. Its antihypertensive activity is revealed in rats with spontaneous and metacorticoid hypertension both after single and chronic applciation of the compound. The influence on the blood pressure weakens after reserpinization of the animals. The compound possesses slight ganglion-blocking action and manifested alpha and beta adreno-blocking activity: diminishes noradrenaline, adrenaline and isoprenaline responses on the arterial pressure and heart activity in anesthetised cats and rats, shifts to the right the cumulative dose-response curve of noradrenaline on vas deferens of a rat, ect. The hypotensive and antihypertensive effect of the compound P11 is discussed on the basis of its alpha and beta adrenoblocking action and its specific sedative effect. | [
-0.02863559126853943,
0.03274565562605858,
-0.03703511133790016,
0.03720087558031082,
0.007104513235390186,
-0.014512146823108196,
0.04874168708920479,
0.03685895353555679,
-0.027983689680695534,
-0.03023642674088478,
0.011290328577160835,
-0.024636099115014076,
-0.0037085479125380516,
-0.007541290950030088,
-0.008140594698488712,
-0.01665819063782692,
0.03399498388171196,
-0.042313240468502045,
-0.060186006128787994,
-0.03559141978621483,
-0.039825763553380966,
0.006320104002952576,
0.04610171541571617,
0.04713089391589165,
0.06012255325913429,
0.011297747492790222,
0.023524170741438866,
0.023704588413238525,
-0.0786714181303978,
-0.16504442691802979,
0.02080254815518856,
0.010078679770231247,
-0.021212531253695488,
0.00835998822003603,
-0.047806620597839355,
0.032785847783088684,
-0.03441132605075836,
0.04174010455608368,
0.004095696844160557,
0.043079398572444916,
0.023028472438454628,
-0.01336342841386795,
-0.024656161665916443,
0.03872411698102951,
0.0036042253486812115,
0.016088541597127914,
-0.05279449746012688,
-0.004993888083845377,
0.10722825676202774,
0.0130785396322608,
0.02536064013838768,
0.01637282781302929,
0.026291927322745323,
0.007521710358560085,
0.04977017268538475,
-0.016445975750684738,
-0.018634427338838577,
0.047364749014377594,
-0.025420481339097023,
0.04762902855873108,
0.05901224538683891,
0.0193276759237051,
-0.17889443039894104,
0.04753732681274414,
0.006371357943862677,
0.028565948829054832,
-0.06414692848920822,
0.007368297316133976,
0.02699328400194645,
0.028334451839327812,
0.009593677707016468,
0.05420202389359474,
-0.020443523302674294,
-0.014067437499761581,
-0.047319091856479645,
-0.027588985860347748,
-0.03833811357617378,
-0.025652335956692696,
0.018974341452121735,
-0.01828426867723465,
0.04488665238022804,
0.010911800898611546,
0.013120216317474842,
-0.023822221904993057,
0.05570012703537941,
-0.01586369425058365,
0.019679894670844078,
-0.00041566183790564537,
-0.02254640683531761,
-0.019024014472961426,
-0.08573150634765625,
0.0041264877654612064,
-0.045489951968193054,
-0.06938683986663818,
-0.02548276260495186,
0.008726503700017929,
-0.04289259761571884,
0.034289635717868805,
-0.008398483507335186,
0.3658911883831024,
0.019010799005627632,
-0.010047299787402153,
-0.09616503864526749,
0.005077695939689875,
0.03167397901415825,
-0.010817105881869793,
-0.014217538759112358,
-0.02282482385635376,
0.016785437241196632,
0.040971603244543076,
0.07911823689937592,
0.03508744388818741,
0.08029672503471375,
-0.025020018219947815,
-0.04630662500858307,
-0.03887157514691353,
-0.0018343573901802301,
0.02869606204330921,
-0.0038898116908967495,
0.022232774645090103,
-0.018883628770709038,
0.011539341881871223,
0.017424074932932854,
-0.017338918522000313,
0.05621546134352684,
0.0015532932011410594,
-0.0032665615435689688,
0.04184293374419212,
0.0643845796585083,
0.0491555854678154,
0.052113667130470276,
0.08518768101930618,
-0.005309349857270718,
0.014818123541772366,
0.007901999168097973,
-0.030294716358184814,
0.014907630160450935,
-0.05388208106160164,
0.05274519324302673,
-0.03674698248505592,
-0.03252312168478966,
-0.06836506724357605,
-0.04667751118540764,
-0.07384909689426422,
-0.062066882848739624,
0.11037363857030869,
0.0786045715212822,
-0.008291056379675865,
-0.007957586087286472,
0.09196726977825165,
-0.012548696249723434,
0.031224431470036507,
0.004426373168826103,
0.04199380800127983,
0.011680681258440018,
-0.0009555111755616963,
0.002707007573917508,
0.01600942388176918,
-0.026295334100723267,
-0.004079034551978111,
-0.08540832251310349,
-0.06355266273021698,
-0.05862697213888168,
0.06486918777227402,
0.030954670161008835,
-0.022113533690571785,
-0.002268863143399358,
-0.01799057424068451,
0.01765351928770542,
0.01071033626794815,
0.02359335497021675,
-0.0009236438781954348,
-0.008554099127650261,
0.0048301503993570805,
-0.06781745702028275,
-0.01610955037176609,
0.026346541941165924,
-0.06974886357784271,
0.05115531012415886,
-0.015068631619215012,
0.04112089425325394,
-0.014483882114291191,
0.0008708836976438761,
-0.007067484315484762,
-0.02075120247900486,
0.04056825116276741,
-0.05198959633708,
-0.042237475514411926,
-0.033107370138168335,
0.02819087915122509,
-0.017351312562823296,
0.009439697489142418,
-0.04081200435757637,
0.03936683386564255,
-0.017450299113988876,
-0.0326216034591198,
-0.07111113518476486,
0.06486533582210541,
-0.03580424189567566,
-0.004178044386208057,
0.10535798966884613,
-0.01579333283007145,
0.01964816078543663,
0.017802603542804718,
0.03339521214365959,
0.047947850078344345,
-0.029125338420271873,
-0.0013341119047254324,
0.004448064137250185,
-0.04990816116333008,
0.04316996783018112,
0.030454430729150772,
0.007206270471215248,
0.0014175035757943988,
-0.04404538497328758,
0.03898319602012634,
-0.05033271387219429,
0.03952440246939659,
0.06685290485620499,
0.04403507336974144,
0.022542111575603485,
-0.04026414453983307,
0.05137081816792488,
-0.32956060767173767,
-0.03857141733169556,
0.02752060256898403,
-0.07282333076000214,
0.05036904662847519,
-0.0228528194129467,
0.027116822078824043,
-0.05852614343166351,
-0.03190110623836517,
-0.032992806285619736,
0.05203299969434738,
0.04204559326171875,
-0.023916032165288925,
-0.040509775280952454,
-0.005208149086683989,
0.07638716697692871,
0.036421582102775574,
-0.05517124384641647,
0.005848613101989031,
-0.007554251234978437,
0.041028037667274475,
0.002651082118973136,
-0.04800739139318466,
-0.0049109929241240025,
-0.03176587447524071,
-0.03961876779794693,
0.1234927698969841,
-0.020486989989876747,
-0.01485330518335104,
0.03353439271450043,
-0.0011301670456305146,
0.05547615885734558,
-0.03059384971857071,
-0.11981897056102753,
-0.006433531641960144,
-0.02502371557056904,
0.013456953689455986,
0.02216283231973648,
-0.04064977169036865,
-0.01490748580545187,
-0.0446753166615963,
0.05058706924319267,
0.02600218914449215,
-0.05426640063524246,
-0.06125006824731827,
-0.028225654736161232,
0.002006326802074909,
0.06046717241406441,
-0.01997849904000759,
-0.009650323539972305,
0.03477456048130989,
0.030348919332027435,
0.010325109586119652,
0.015272432006895542,
0.012263085693120956,
0.021842529997229576,
-0.0967487096786499,
0.008555950596928596,
-0.00023056568170432,
-0.0691567063331604,
-0.004752420820295811,
0.024997306987643242,
0.054381273686885834,
0.01621672697365284,
-0.04596839100122452,
0.058695171028375626,
0.002723801415413618,
-0.028332548215985298,
0.04542970284819603,
0.05365515127778053,
-0.023601554334163666,
0.11987272650003433,
0.007173334248363972,
0.052086155861616135,
0.015187647193670273,
-0.020524242892861366,
-0.0021615459118038416,
-0.04898451268672943,
-0.023971503600478172,
0.04396703466773033,
-0.04591371864080429,
-0.03209959715604782,
0.024445008486509323,
0.00466354051604867,
-0.02918063849210739,
-0.041982099413871765,
0.0061530922539532185,
-0.0069719646126031876,
0.0442977137863636,
-0.026748497039079666,
0.03115515410900116,
-0.028851298615336418,
-0.00209839572198689,
-0.0026752175763249397,
0.05614737793803215,
0.01972673088312149,
-0.28963419795036316,
-0.005030525382608175,
0.037162020802497864,
-0.036654867231845856,
-0.011150517500936985,
0.012435412965714931,
0.016079822555184364,
-0.0515633188188076,
-0.06867195665836334,
-0.024957137182354927,
-0.02583291195333004,
0.04841449111700058,
0.07187788188457489,
0.043206777423620224,
-0.054468605667352676,
-0.024116970598697662,
0.04421805217862129,
0.02230982296168804,
0.017418088391423225,
0.0048114038072526455,
0.028694314882159233,
-0.039963677525520325,
0.14260326325893402,
0.006425817031413317,
-0.02584652416408062,
0.06127080321311951,
0.011205313727259636,
-0.003453266341239214,
-0.026175877079367638,
0.02030155248939991,
-0.02938280813395977,
0.019474701955914497,
0.09922488033771515,
-0.06604122370481491,
0.027845891192555428,
0.026677558198571205,
-0.05448179692029953,
0.013526178896427155,
-0.03298655152320862,
-0.036442749202251434,
-0.014594725333154202,
0.016616448760032654,
-0.03653595224022865,
0.01341176312416792,
0.0637902244925499,
-0.08050370216369629,
-0.06238611415028572,
0.014204337261617184,
-0.0010108532151207328,
0.038088109344244,
-0.033780764788389206,
-0.02001454494893551,
0.05024734511971474,
0.014518516138195992,
0.004416400101035833,
-0.02741827815771103,
-0.02454594150185585,
-0.049117378890514374,
0.03800129517912865,
-0.03327837213873863,
0.007982165552675724,
0.010210545733571053,
0.029475737363100052,
0.04916420951485634,
0.05382593348622322
] |
PMID:25166 | Effects of local delayed hypersensitivity on the small intestine. | There are many T and B cells in the small intestinal mucosa and local T cell immunity could have a role both in protective immunity and as a cause of disease (i.e. hypersensitivity). This latter aspect has been investigated by using several animal models to assess the effects of local delayed hypersensitivity on the structure and function of the small intestine. Heterotopically transplanted grafts of fetal small intestine in mice (isografts and allografts) have been examined by conventional histology, scanning and transmission electron microscopy, by making direct measurements of villi, crypts, and lymphoid cell infiltrate, and by counting the number of mitoses per crypt. This cell-mediated immune reaction causes lymphocyte infiltration which is most marked in the lamina propria, hyperplasia of the crypts of Lieberkühn, increased cell loss with villous atrophy and a flat surface, but the individual enterocytes appear fairly normal. Graft-versus-host disease cause exactly the same changes in structure and in cell kinetics as does rejection. However, crypt hyperplasia has been found to precede villous atrophy by several days. Preliminary experiments on local contact hypersensitivity suggest that intraluminal injection of oxazolone in the gut of sensitized mice also produces villous atrophy and crypt hyperplasia. It is postulated that these effects are likely to be produced via lymphokines: by an 'enteropathic' factor which damages the lamina propria and basement membrane, and a factor which is mitogenic for crypt stem cells. In mice infected with Giardia lamblia, crypt hyperplasia and lymphocyte infiltration of the epithelium are present and there is accelerated epithelial cell turnover. In rats infected with Nippostrongylus brasiliensis, the flat mucosa has been shown to be due to the thymus-dependent immune response and not directly to the damage produced by the parasite itself. A common factor in the variety of conditions associated with villous atrophy and crypt hyperplasia may well be a local cell-mediated immune reaction to food, microbial, parasite or other antigens which causes changes in enterocyte turnover rate and malabsorption. | Effects of local delayed hypersensitivity on the small intestine. There are many T and B cells in the small intestinal mucosa and local T cell immunity could have a role both in protective immunity and as a cause of disease (i.e. hypersensitivity). This latter aspect has been investigated by using several animal models to assess the effects of local delayed hypersensitivity on the structure and function of the small intestine. Heterotopically transplanted grafts of fetal small intestine in mice (isografts and allografts) have been examined by conventional histology, scanning and transmission electron microscopy, by making direct measurements of villi, crypts, and lymphoid cell infiltrate, and by counting the number of mitoses per crypt. This cell-mediated immune reaction causes lymphocyte infiltration which is most marked in the lamina propria, hyperplasia of the crypts of Lieberkühn, increased cell loss with villous atrophy and a flat surface, but the individual enterocytes appear fairly normal. Graft-versus-host disease cause exactly the same changes in structure and in cell kinetics as does rejection. However, crypt hyperplasia has been found to precede villous atrophy by several days. Preliminary experiments on local contact hypersensitivity suggest that intraluminal injection of oxazolone in the gut of sensitized mice also produces villous atrophy and crypt hyperplasia. It is postulated that these effects are likely to be produced via lymphokines: by an 'enteropathic' factor which damages the lamina propria and basement membrane, and a factor which is mitogenic for crypt stem cells. In mice infected with Giardia lamblia, crypt hyperplasia and lymphocyte infiltration of the epithelium are present and there is accelerated epithelial cell turnover. In rats infected with Nippostrongylus brasiliensis, the flat mucosa has been shown to be due to the thymus-dependent immune response and not directly to the damage produced by the parasite itself. A common factor in the variety of conditions associated with villous atrophy and crypt hyperplasia may well be a local cell-mediated immune reaction to food, microbial, parasite or other antigens which causes changes in enterocyte turnover rate and malabsorption. | [
-0.01872299611568451,
-0.004696496296674013,
0.008705733343958855,
0.08903870731592178,
-0.005165094509720802,
-0.024582771584391594,
0.04866577684879303,
0.09118887782096863,
-0.053341325372457504,
-0.0614142119884491,
0.02398964948952198,
-0.07712842524051666,
0.02105158381164074,
-0.07180704176425934,
-0.014451988972723484,
-0.004921796731650829,
-0.037062644958496094,
-0.024426307529211044,
-0.11628745496273041,
-0.03054749220609665,
-0.0436529666185379,
-0.036760229617357254,
0.03097301349043846,
-0.035147298127412796,
0.04569806531071663,
-0.04737924039363861,
-0.012809490785002708,
0.004362190142273903,
-0.07594860345125198,
-0.1740671843290329,
0.010699283331632614,
-0.008923597633838654,
0.037558648735284805,
0.0313422828912735,
-0.030854033306241035,
-0.038593392819166183,
-0.002453447785228491,
-0.005402382928878069,
0.02335161715745926,
0.013890333473682404,
0.040219247341156006,
0.06751241534948349,
0.030072353780269623,
0.014110952615737915,
0.019603250548243523,
-0.019715046510100365,
-0.02768152952194214,
-0.08749289810657501,
0.08831794559955597,
0.005037814378738403,
0.04377453029155731,
-0.027949988842010498,
-0.0010385093046352267,
0.10655492544174194,
-0.007977906614542007,
0.023801879957318306,
0.010403969325125217,
0.02317945286631584,
0.046583570539951324,
0.05078966170549393,
0.043008048087358475,
0.032445404678583145,
-0.1036621481180191,
0.07359834760427475,
0.08005937188863754,
-0.01742096059024334,
-0.03945652395486832,
-0.008721889927983284,
0.0661868155002594,
0.0016130809672176838,
-0.03514886647462845,
0.0485062412917614,
0.017559895291924477,
0.0674576386809349,
-0.0008878295193426311,
-0.018819935619831085,
-0.011775086633861065,
-0.018169758841395378,
0.030891748145222664,
0.034422457218170166,
0.05467076972126961,
0.04152863472700119,
-0.013082964345812798,
-0.010614198632538319,
-0.038002219051122665,
-0.04160746932029724,
-0.0011072371853515506,
-0.06538645923137665,
-0.020710458979010582,
-0.010064291767776012,
-0.018210142850875854,
-0.062198661267757416,
-0.022794919088482857,
0.010146090760827065,
-0.04082364961504936,
-0.0023521301336586475,
0.0039005789440125227,
-0.044267281889915466,
0.010855116881430149,
0.37845420837402344,
-0.020689748227596283,
0.0501270666718483,
-0.05933894217014313,
0.026321938261389732,
0.031874317675828934,
0.03372294083237648,
0.03397057577967644,
0.0007217876263894141,
-0.03425228223204613,
0.09093117713928223,
0.049863044172525406,
0.03449859842658043,
-0.04426615685224533,
0.04939037561416626,
-0.0033474622759968042,
-0.020862290635704994,
0.09307383000850677,
-0.021739693358540535,
-0.02399194985628128,
0.08835536241531372,
0.005592759698629379,
0.014207062311470509,
0.0028677196241915226,
-0.08291914314031601,
0.03179413080215454,
0.05965337157249451,
0.005414628889411688,
0.0920456126332283,
0.02865712158381939,
-0.0071821738965809345,
0.010635234415531158,
0.008101950399577618,
-0.028343746438622475,
0.012175985611975193,
-0.006386591121554375,
-0.0033477263059467077,
0.02032030187547207,
-0.0217480156570673,
-0.02334517054259777,
0.005885673221200705,
-0.017687223851680756,
-0.07273354381322861,
-0.026968806982040405,
-0.08427039533853531,
-0.010136755183339119,
0.07970677316188812,
0.01787167601287365,
-0.012697570957243443,
-0.035164520144462585,
-0.024767985567450523,
-0.015140841715037823,
0.003173133125528693,
-0.005074949935078621,
0.020591499283909798,
-0.039550915360450745,
0.0007588685257360339,
-0.015295885503292084,
0.027174312621355057,
0.0007812121184542775,
-0.07301665842533112,
-0.03130319342017174,
-0.009043565951287746,
-0.025558345019817352,
0.06554708629846573,
0.0007636175723746419,
0.00612590741366148,
-0.026043087244033813,
-0.04270046949386597,
0.0408511720597744,
0.015046306885778904,
0.0007788529619574547,
0.02678874135017395,
-0.034751784056425095,
-0.05549844354391098,
-0.051631860435009,
0.05354289337992668,
-0.0692010298371315,
-0.0017230419907718897,
0.005418040324002504,
0.046599794179201126,
-0.03072414919734001,
-0.012797912582755089,
-0.01787346415221691,
-0.014657247811555862,
0.027683841064572334,
0.01692873053252697,
-0.019984934478998184,
0.019108891487121582,
0.005066894926130772,
-0.00024222992942668498,
-0.0074476683512330055,
-0.035418640822172165,
-0.12984153628349304,
-0.02546081505715847,
-0.01781899482011795,
-0.03837791830301285,
-0.01265279483050108,
-0.020272433757781982,
-0.014795832335948944,
-0.04115118086338043,
0.05039057508111,
0.013496981002390385,
0.026678891852498055,
0.024036196991801262,
-0.013553950935602188,
0.014584857039153576,
0.09305574744939804,
-0.06769274920225143,
0.04640622437000275,
-0.015362311154603958,
-0.0624191015958786,
0.06407781690359116,
0.00886140763759613,
-0.001348756835795939,
0.02960970439016819,
0.0012593237916007638,
-0.01627391204237938,
-0.007765292655676603,
0.1062408983707428,
-0.025800639763474464,
0.0729079470038414,
0.005025271791964769,
-0.021711140871047974,
-0.30968010425567627,
-0.09914420545101166,
-0.02839915081858635,
-0.04202830791473389,
-0.011946065351366997,
0.003643232164904475,
-0.007872192189097404,
-0.05518006533384323,
0.01900395192205906,
-0.018656402826309204,
0.0972297191619873,
0.08463969826698303,
-0.016252800822257996,
-0.03556988015770912,
-0.02403579279780388,
0.038067080080509186,
0.039862245321273804,
0.0008248521480709314,
0.05463864281773567,
-0.010519467294216156,
0.016341783106327057,
0.005742181092500687,
0.017273833975195885,
-0.004660369362682104,
-0.005964880343526602,
0.003287023864686489,
0.09702657908201218,
0.004803381860256195,
-0.010470706969499588,
-0.024873504415154457,
0.015556630678474903,
-0.01732633449137211,
-0.016916582360863686,
-0.03443034738302231,
0.043352045118808746,
0.004370264243334532,
-0.01978420279920101,
-0.022928697988390923,
-0.013187221251428127,
-0.009829549118876457,
-0.06368091702461243,
-0.0284641832113266,
-0.016956917941570282,
-0.07260695099830627,
-0.001990383956581354,
0.030963042750954628,
0.03254194185137749,
0.05968982353806496,
0.0174177847802639,
-0.012180574238300323,
0.06024390086531639,
-0.04427153617143631,
0.011792344972491264,
0.04309438169002533,
0.04790174961090088,
-0.028403611853718758,
0.02189553901553154,
-0.017591189593076706,
-0.08634236454963684,
0.008050723932683468,
-0.00828590802848339,
-0.027106963098049164,
0.04943855106830597,
-0.019590172916650772,
0.0031249707099050283,
0.039813365787267685,
0.0036627850495278835,
-0.01603827439248562,
0.034572601318359375,
0.0016893669962882996,
0.024582402780652046,
0.14937059581279755,
0.007213853765279055,
0.005047318991273642,
-0.024767685681581497,
0.022682271897792816,
0.028950275853276253,
-0.016243457794189453,
-0.059333983808755875,
-0.020432960242033005,
0.039571844041347504,
-0.045622438192367554,
0.03803242743015289,
0.04744117707014084,
-0.001241785939782858,
0.011882014572620392,
0.0024667929392307997,
-0.0029226590413600206,
-0.01605728268623352,
-0.013515554368495941,
0.020636294037103653,
0.03638741001486778,
0.008631817065179348,
0.02361716516315937,
-0.026400305330753326,
0.012691023759543896,
-0.29752466082572937,
0.01582358591258526,
0.027542755007743835,
-0.034913402050733566,
-0.049729738384485245,
0.027224481105804443,
0.012726212851703167,
-0.057697832584381104,
-0.02532532811164856,
0.01899334229528904,
0.05272781848907471,
0.02362813800573349,
0.11472059041261673,
-0.008339482359588146,
-0.03800947591662407,
-0.027054401114583015,
0.0272739939391613,
0.0092707434669137,
-0.02002992480993271,
-0.016246818006038666,
0.009253625757992268,
-0.007933619432151318,
0.17010840773582458,
0.016483258455991745,
-0.022125517949461937,
0.0021124316845089197,
0.022771529853343964,
0.06415106356143951,
0.015086643397808075,
-0.03608416020870209,
0.017680343240499496,
0.022865375503897667,
0.012530769221484661,
0.049550797790288925,
-0.00545309716835618,
-0.026102110743522644,
-0.01743299327790737,
0.0032224170863628387,
-0.06487780064344406,
0.010303010232746601,
-0.033611077815294266,
0.02982480451464653,
-0.03953976556658745,
-0.022625461220741272,
0.05038639158010483,
-0.05133059620857239,
-0.0059029720723629,
-0.035750336945056915,
0.0031110644340515137,
0.03586285561323166,
-0.07016512006521225,
0.005105567630380392,
0.048266202211380005,
-0.02132316492497921,
0.010375171899795532,
-0.041558243334293365,
0.036143410950899124,
-0.015057443641126156,
-0.004163863603025675,
0.04340967908501625,
0.018246209248900414,
-0.03478886932134628,
-0.07627390325069427,
0.07027891278266907,
0.04879572615027428
] |
PMID:25171 | [Study of a beta-adrenergic stimulant and its antidepressant activity in man]. | In animals, the psychopharmacological profile of beta-adrenergic stimulants is very similar to that of tricyclic antidepressants. In patients, more particularly in endogenous depressive patients, the antidepressant effect of salbutamol was very clear. A definite improvement was observed in all of the 22 studied patients after 1 to 3 days of intermittent venous infusion. The place of salbutamol in the therapeutic armamentarium of depressive states has still to be defined exactly. It is speculated that the antidepressant effect of imipramine-like derivatives is related to the stimulation of central beta 2-adrenergic receptors. | [Study of a beta-adrenergic stimulant and its antidepressant activity in man]. In animals, the psychopharmacological profile of beta-adrenergic stimulants is very similar to that of tricyclic antidepressants. In patients, more particularly in endogenous depressive patients, the antidepressant effect of salbutamol was very clear. A definite improvement was observed in all of the 22 studied patients after 1 to 3 days of intermittent venous infusion. The place of salbutamol in the therapeutic armamentarium of depressive states has still to be defined exactly. It is speculated that the antidepressant effect of imipramine-like derivatives is related to the stimulation of central beta 2-adrenergic receptors. | [
0.0037859752774238586,
0.021981270983815193,
-0.010584545321762562,
0.019941437989473343,
0.014797603711485863,
0.053498074412345886,
0.06047238036990166,
0.039144307374954224,
-0.015806734561920166,
-0.11766067892313004,
0.01661125384271145,
0.006529887672513723,
0.02175532653927803,
0.06298710405826569,
-0.02701052650809288,
0.01739361509680748,
0.053483493626117706,
-0.04290584474802017,
-0.03723413869738579,
-0.056350819766521454,
-0.04953208565711975,
0.013655008748173714,
-0.001306745340116322,
-0.05202220380306244,
-0.022206725552678108,
0.02635609358549118,
-0.004393755458295345,
0.0072854734025895596,
-0.0711221992969513,
-0.13631673157215118,
0.03197615221142769,
0.039509497582912445,
0.05189429223537445,
-0.05230303108692169,
-0.037108585238456726,
0.004039832390844822,
-0.0026802713982760906,
0.04465729743242264,
-0.04557396471500397,
0.04166688397526741,
0.041915230453014374,
-0.007155625615268946,
-0.005445728078484535,
0.040193308144807816,
-0.007807718589901924,
-0.03479445353150368,
-0.033959392458200455,
-0.05396050959825516,
0.11281736195087433,
-0.003072969615459442,
0.05547824129462242,
-0.015777606517076492,
0.030236700549721718,
0.019097063690423965,
0.03605980798602104,
-0.005680370144546032,
-0.0710630789399147,
0.06503626704216003,
-0.044838033616542816,
0.014831515029072762,
-0.028011854737997055,
0.055571481585502625,
-0.1624993085861206,
0.02441350743174553,
0.02339218370616436,
0.009522558189928532,
-0.08376461267471313,
-0.014172660186886787,
0.026773903518915176,
0.03088725171983242,
-0.02296946570277214,
0.030439535155892372,
0.009822551161050797,
-0.015120076946914196,
0.006598158739507198,
-0.0036733467131853104,
-0.039870258420705795,
0.003082766430452466,
0.005175408441573381,
-0.029066042974591255,
0.09258352965116501,
-0.006694875191897154,
0.001603337237611413,
-0.044815391302108765,
-0.016941554844379425,
-0.005696280859410763,
0.08889225125312805,
-0.038172464817762375,
0.0003934474370907992,
-0.04425233229994774,
-0.033338502049446106,
-0.01150312926620245,
0.012213853187859058,
-0.020023660734295845,
-0.0019859294407069683,
0.012462682090699673,
-0.03140822425484657,
-0.030910011380910873,
0.033392950892448425,
0.3337779641151428,
0.012015136890113354,
0.05625709146261215,
-0.05757611617445946,
-0.000991777633316815,
0.024242481216788292,
0.02295934408903122,
-0.004971943795681,
-0.030075769871473312,
-0.010800368152558804,
0.00822314340621233,
0.031324926763772964,
-0.031845156103372574,
0.10645776987075806,
-0.024937838315963745,
-0.05512731522321701,
-0.048208240419626236,
0.007537302561104298,
-0.030818702653050423,
0.013170045800507069,
0.005584580823779106,
-0.039114776998758316,
0.0026838663034141064,
-0.0033131015952676535,
-0.0296946931630373,
0.004580378532409668,
-0.007775967475026846,
-0.038159679621458054,
0.05815786123275757,
0.07782842218875885,
-0.06633677333593369,
0.04984108731150627,
0.03396131843328476,
-0.03661654144525528,
0.04791940003633499,
0.020319171249866486,
0.010945732705295086,
0.0027509487699717283,
0.03571874648332596,
0.05615014582872391,
-0.01932905986905098,
0.03434450551867485,
-0.04941491037607193,
0.029446424916386604,
-0.07486669719219208,
-0.06421887129545212,
0.08585109561681747,
0.07385861128568649,
-0.05274461209774017,
-0.04126299172639847,
0.05459201708436012,
0.0005284211365506053,
0.045214131474494934,
-0.0015265285037457943,
0.030958613380789757,
-0.017896581441164017,
0.021237242966890335,
0.08317077904939651,
0.025101231411099434,
-0.03482155501842499,
-0.00687577947974205,
-0.03094981424510479,
-0.03299430385231972,
0.013853678479790688,
0.038210492581129074,
-0.01987450197339058,
0.027450526133179665,
-0.06887141615152359,
-0.028370674699544907,
-0.010949421674013138,
0.011733267456293106,
0.005940703209489584,
-0.03208369389176369,
-0.0467245876789093,
-0.06410297751426697,
-0.05648127943277359,
-0.012006839737296104,
0.09418707340955734,
0.004917575512081385,
0.02008819580078125,
-0.05064292624592781,
0.0201950091868639,
-0.004028133116662502,
0.02314540185034275,
-0.00510666286572814,
0.003045654622837901,
0.02525990828871727,
0.019321193918585777,
0.004419040400534868,
0.012672950513660908,
-0.015080277808010578,
-0.03805040195584297,
0.011363537982106209,
-0.051296766847372055,
0.009366365149617195,
-0.034120138734579086,
-0.03386182710528374,
-0.029336808249354362,
-0.008842885494232178,
-0.005023441277444363,
0.04877772182226181,
0.04506593197584152,
0.08946184068918228,
-0.00239962013438344,
0.025364188477396965,
0.06439003348350525,
0.041654638946056366,
-0.01175988744944334,
-0.010280417278409004,
0.06492065638303757,
-0.035613033920526505,
0.022125206887722015,
0.059351783245801926,
0.04710875824093819,
-0.03818245977163315,
-0.021632971242070198,
0.04477216303348541,
-0.017201855778694153,
-0.009593509137630463,
0.08485762774944305,
-0.014813641086220741,
-0.0019349191570654511,
-0.002042369218543172,
-0.022358117625117302,
-0.28672951459884644,
-0.05408133566379547,
-0.003785902401432395,
-0.007848675362765789,
0.014300762675702572,
-0.07108324021100998,
0.0032234597019851208,
-0.060987409204244614,
0.005471902433782816,
-0.059410374611616135,
0.04381413757801056,
-0.027754751965403557,
0.006679942831397057,
-0.0465138740837574,
0.05048607662320137,
0.023268401622772217,
0.04841708764433861,
-0.07732414454221725,
0.015300444327294827,
0.039270032197237015,
0.018737193197011948,
0.0029378090985119343,
0.00792016088962555,
0.02238868921995163,
0.05579734221100807,
-0.054239433258771896,
0.08015982061624527,
-0.012413784861564636,
-0.017862442880868912,
0.03993332013487816,
-0.015161647461354733,
0.08669614791870117,
0.01991397887468338,
-0.16087444126605988,
-0.02243255078792572,
-0.03655978664755821,
0.05587305501103401,
-0.017952285706996918,
0.015533339232206345,
0.046083804219961166,
-0.038537055253982544,
0.02422342076897621,
0.0770251452922821,
-0.04102962836623192,
-0.038693804293870926,
0.0005193175747990608,
0.045374270528554916,
-0.0006664243992418051,
-0.06677354127168655,
0.02198679745197296,
-0.014406263828277588,
0.010535657405853271,
-0.0036484086886048317,
-0.025504818186163902,
0.006799448747187853,
0.01252006459981203,
-0.11267078667879105,
-0.0506567619740963,
-0.018928298726677895,
-0.02568725124001503,
0.015655219554901123,
0.024741875007748604,
0.02540018782019615,
0.002417945768684149,
-0.018030505627393723,
0.01867172122001648,
0.030509445816278458,
-0.04568380117416382,
0.035228218883275986,
0.04413089156150818,
0.04868840053677559,
0.14233720302581787,
-0.03634322062134743,
0.02999269776046276,
0.08105064928531647,
-0.003325073514133692,
-0.01378272008150816,
-0.01662430353462696,
-0.08796516805887222,
0.037100955843925476,
-0.06163764372467995,
-0.054227910935878754,
0.025745876133441925,
0.01463727280497551,
0.020210055634379387,
-0.023256754502654076,
0.002121219877153635,
-0.015845635905861855,
0.07350946217775345,
0.003829382825642824,
-0.04128251597285271,
-0.013057501055300236,
-0.01658131740987301,
-0.02806183509528637,
-0.007824905216693878,
0.0666818916797638,
-0.28320810198783875,
-0.01442437618970871,
-0.02630595862865448,
-0.035345759242773056,
0.05333646386861801,
0.027404379099607468,
0.022767435759305954,
-0.06333889812231064,
-0.03304969146847725,
-0.06159788742661476,
0.0023655076511204243,
0.05154699832201004,
0.07588993012905121,
0.008068886585533619,
-0.056381601840257645,
0.0030204178765416145,
0.028722308576107025,
0.007640593219548464,
0.038520216941833496,
-0.08094906806945801,
0.004180956166237593,
0.04448946937918663,
0.11849019676446915,
0.007470665033906698,
-0.007622422184795141,
0.01084290724247694,
0.010011791251599789,
-0.007839705795049667,
-0.07763059437274933,
0.007443139795213938,
-0.008890966884791851,
-0.005495300982147455,
0.1067783385515213,
-0.00831315666437149,
0.007643885910511017,
-0.005001956131309271,
-0.08813498169183731,
0.005334837362170219,
0.06431856751441956,
0.04310925304889679,
-0.0031188279390335083,
0.03136017546057701,
-0.020507529377937317,
-0.018261253833770752,
0.12413200736045837,
-0.07562651485204697,
-0.05841362848877907,
-0.002462340984493494,
0.010868559591472149,
-0.00032443017698824406,
0.004846208728849888,
-0.03328932076692581,
0.019463514909148216,
0.02568061463534832,
-0.011799502186477184,
0.008674314245581627,
-0.03794466704130173,
-0.015516093000769615,
0.034129220992326736,
-0.03540804237127304,
-0.06347578763961792,
0.029727285727858543,
-0.03175956755876541,
0.03296926990151405,
0.13014814257621765
] |
PMID:25172 | Apparent effects of neurotransmitters on sexual differentiation of the brain without mediation of sex hormones. | Male sexual behaviour was found to be permanently decreased in neonatally reserpinized or paragylinized male rats. On the other hand, hypoplasia of sex organs was only observed in reserpinized, but not in pargylinized newborn males. Furthermore, male sexual behavior was found to be permanently increased in neonatally pyridostigminized males which showed even a slight hypoplasia of seminal vesicles in neonatal life. These findings suggest that changes of neurotransmitter concentrations and/or turnover rates apparently induced by psychotrophic drugs can affect sex-specific brain differentiation by direct action without mediation of sex hormones. Hence, neurotransmitters may be regarded as organizers of the brain. | Apparent effects of neurotransmitters on sexual differentiation of the brain without mediation of sex hormones. Male sexual behaviour was found to be permanently decreased in neonatally reserpinized or paragylinized male rats. On the other hand, hypoplasia of sex organs was only observed in reserpinized, but not in pargylinized newborn males. Furthermore, male sexual behavior was found to be permanently increased in neonatally pyridostigminized males which showed even a slight hypoplasia of seminal vesicles in neonatal life. These findings suggest that changes of neurotransmitter concentrations and/or turnover rates apparently induced by psychotrophic drugs can affect sex-specific brain differentiation by direct action without mediation of sex hormones. Hence, neurotransmitters may be regarded as organizers of the brain. | [
0.027025043964385986,
0.01116645522415638,
0.03758153319358826,
0.005439776927232742,
-0.02245723456144333,
0.09023826569318771,
0.0694073885679245,
0.021208615973591805,
-0.02671920694410801,
-0.03447623550891876,
0.02172856032848358,
-0.0025521160569041967,
0.035470686852931976,
0.06533592939376831,
-0.05279127135872841,
0.009182212874293327,
-0.06059781461954117,
0.017057863995432854,
-0.05978769063949585,
-0.0033254665322601795,
0.015328478999435902,
-0.016881560906767845,
0.05753585696220398,
-0.021086817607283592,
-0.012694320641458035,
-0.017873534932732582,
-0.022127658128738403,
0.016901304945349693,
-0.04586205258965492,
-0.15566839277744293,
0.006182449869811535,
-0.0007856430020183325,
-0.020133212208747864,
-0.04779573902487755,
0.0064354450441896915,
0.015289003029465675,
-0.000034538181353127584,
0.0206339992582798,
0.0013571358285844326,
0.0534479059278965,
-0.006706163752824068,
-0.022840064018964767,
-0.010952998884022236,
0.03208599239587784,
0.004536886233836412,
0.027245625853538513,
-0.027227148413658142,
-0.024766888469457626,
0.008709385991096497,
-0.05171744152903557,
0.010180647484958172,
-0.04424259811639786,
0.058443449437618256,
0.0643693059682846,
-0.032189108431339264,
0.01136346161365509,
0.03997776284813881,
0.007422456983476877,
0.0009838033001869917,
0.047019608318805695,
-0.045095931738615036,
0.048063382506370544,
-0.13632921874523163,
0.0004361378960311413,
0.08236707746982574,
-0.03521942347288132,
-0.051972053945064545,
-0.01794382557272911,
0.057504910975694656,
-0.005295395385473967,
-0.004327470436692238,
0.021008169278502464,
0.02053290419280529,
-0.04636557772755623,
0.04177726432681084,
-0.09104839712381363,
-0.06491029262542725,
-0.05077766254544258,
0.032753344625234604,
0.010580574162304401,
0.013579977676272392,
0.034162405878305435,
0.0216500386595726,
-0.06586915254592896,
-0.0349891297519207,
-0.05084750056266785,
-0.02348322793841362,
-0.06251737475395203,
-0.06509781628847122,
0.03360266610980034,
-0.00965103879570961,
-0.021776331588625908,
-0.002586100483313203,
-0.028652558103203773,
-0.016130762174725533,
0.06737834960222244,
-0.013331489637494087,
-0.05327282473444939,
-0.006942254025489092,
0.40359681844711304,
0.015600640326738358,
-0.003433753037825227,
-0.032879628241062164,
0.0469529926776886,
-0.03833072632551193,
0.05403871089220047,
-0.0032627726905047894,
-0.03574410825967789,
-0.039626095443964005,
0.07915009558200836,
0.006367065943777561,
0.03166719153523445,
0.06354577839374542,
-0.02210478112101555,
-0.053760405629873276,
-0.08239035308361053,
0.07957363873720169,
0.04850728064775467,
0.008445346727967262,
0.009185153990983963,
-0.046824242919683456,
0.030682308599352837,
0.088878333568573,
0.004270348697900772,
0.06713639199733734,
-0.011725887656211853,
-0.06734319031238556,
0.09043562412261963,
0.030130308121442795,
-0.02656329981982708,
0.08236272633075714,
0.07250760495662689,
0.010196841321885586,
0.0655839815735817,
0.011699448339641094,
-0.050384700298309326,
0.014930140227079391,
-0.016724463552236557,
-0.005927387624979019,
-0.029139557853341103,
-0.024879414588212967,
-0.019588908180594444,
0.010672603733837605,
-0.06150278076529503,
-0.04956696182489395,
0.07502363622188568,
0.041731201112270355,
-0.04584288224577904,
-0.028066108003258705,
0.10437940061092377,
-0.010018011555075645,
0.06317967176437378,
-0.013053767383098602,
0.035893410444259644,
-0.04222238436341286,
0.000263426685705781,
-0.0035284676123410463,
-0.03926130011677742,
-0.0671820119023323,
0.010919506661593914,
0.0029178496915847063,
-0.008919425308704376,
-0.019674744457006454,
0.0740995854139328,
0.05777163431048393,
0.010364847257733345,
-0.01943497359752655,
-0.016965428367257118,
0.011891848407685757,
-0.034301288425922394,
-0.0447143130004406,
0.04315997287631035,
-0.006177245639264584,
-0.01595941185951233,
-0.04695078730583191,
-0.037678252905607224,
0.05612577497959137,
-0.011270087212324142,
-0.0037432846147567034,
0.00973910465836525,
0.06362783908843994,
-0.11819310486316681,
-0.019774528220295906,
0.012385448440909386,
0.021310878917574883,
-0.008507288992404938,
-0.04932398349046707,
0.015465037897229195,
0.043176114559173584,
0.007183942012488842,
-0.007079014088958502,
-0.0011609380599111319,
-0.03696734830737114,
-0.015196166932582855,
-0.008983252570033073,
-0.014670398086309433,
-0.06425777822732925,
-0.025898046791553497,
-0.014195327647030354,
-0.015426397323608398,
0.029716238379478455,
-0.00987345352768898,
0.0310815516859293,
0.050553809851408005,
-0.025744209066033363,
0.05974436551332474,
-0.026932571083307266,
-0.049171190708875656,
0.08959329128265381,
0.016145139932632446,
-0.05945023149251938,
-0.024005567654967308,
0.009669375605881214,
-0.011737488210201263,
-0.022193018347024918,
0.022673724219202995,
0.0068983095698058605,
-0.025429464876651764,
0.08722368627786636,
0.0010188999585807323,
0.00378624745644629,
-0.04349980503320694,
-0.0057290117256343365,
-0.2810622751712799,
-0.08483396470546722,
0.059245094656944275,
-0.010495949536561966,
0.047127075493335724,
-0.08222566545009613,
0.022961601614952087,
-0.03428443893790245,
0.04963818937540054,
-0.0018601341871544719,
0.018291639164090157,
0.07841653376817703,
-0.04582848772406578,
0.0334826335310936,
0.009155159816145897,
0.03534461185336113,
0.04842892661690712,
-0.024994706735014915,
0.00134765706025064,
0.0006888990756124258,
0.004048316273838282,
0.010368305258452892,
-0.027526678517460823,
-0.01497634407132864,
0.037426382303237915,
-0.027877403423190117,
0.07682084292173386,
0.029213393107056618,
0.026702847331762314,
-0.013724878430366516,
0.009202834218740463,
0.021151648834347725,
-0.03242858871817589,
-0.11400031298398972,
0.047513969242572784,
-0.010749509558081627,
-0.0224013514816761,
-0.022499652579426765,
-0.01258073840290308,
-0.00820193812251091,
-0.05396704003214836,
0.022508326917886734,
0.0011862358078360558,
0.004513336345553398,
-0.06288610398769379,
-0.005173435900360346,
0.0006248877034522593,
-0.03845405951142311,
0.04927864298224449,
-0.0286858007311821,
0.010830149054527283,
-0.030006147921085358,
-0.016467563807964325,
-0.004346632398664951,
-0.03161778301000595,
-0.02771110273897648,
-0.05153510347008705,
0.005172832868993282,
-0.04842384159564972,
-0.013822795823216438,
0.03660232573747635,
0.002159331226721406,
0.01629418134689331,
-0.06140214949846268,
-0.017473291605710983,
0.04950947314500809,
-0.0057954504154622555,
0.0009613021975383162,
0.0104150315746665,
0.028885988518595695,
0.0848323181271553,
0.1466377228498459,
0.03750935196876526,
0.03957929089665413,
0.049372266978025436,
-0.004570275079458952,
-0.051740001887083054,
-0.029238078743219376,
-0.06671521812677383,
0.0406302809715271,
-0.059170614928007126,
-0.012575502507388592,
0.052744410932064056,
0.03877613693475723,
-0.02575461007654667,
-0.0320122167468071,
-0.01690748892724514,
-0.06054011732339859,
0.05569635331630707,
0.00404211925342679,
-0.013626780360937119,
0.06063167378306389,
-0.0409574881196022,
-0.03477352485060692,
0.04495912045240402,
0.03924509137868881,
-0.2666698694229126,
0.010089174844324589,
0.05653133615851402,
-0.0023655432742089033,
-0.0015323810512199998,
0.0833665058016777,
-0.010995323769748211,
0.0007323463214561343,
-0.009668068028986454,
0.04930618405342102,
0.030968377366662025,
-0.01569679006934166,
0.07467086613178253,
0.023932935670018196,
-0.037127964198589325,
0.04313189163804054,
0.055186677724123,
-0.03885257616639137,
-0.0022079371847212315,
-0.025029581040143967,
0.037266913801431656,
-0.006933736614882946,
0.146800696849823,
0.008060641586780548,
-0.024272501468658447,
0.005076873581856489,
0.004143215250223875,
0.004206342622637749,
0.001703142188489437,
-0.02195298671722412,
-0.0711202323436737,
0.09116004407405853,
0.0714552029967308,
-0.052031032741069794,
0.02438507042825222,
0.04591691121459007,
-0.006605002097785473,
-0.03551951050758362,
0.029780879616737366,
-0.03701423853635788,
-0.014032105915248394,
0.014021836221218109,
-0.045977458357810974,
0.01554834097623825,
0.09960297495126724,
-0.06351863592863083,
0.005962868686765432,
-0.019725032150745392,
-0.008296534419059753,
0.017122114077210426,
0.006365109700709581,
-0.03709270805120468,
0.015745338052511215,
0.06765271723270416,
0.008865032345056534,
-0.0012489133514463902,
-0.03202708810567856,
-0.04011891782283783,
0.016653170809149742,
-0.016491416841745377,
-0.033783357590436935,
-0.01662539876997471,
-0.04728825390338898,
0.06579112261533737,
-0.00650416174903512
] |
PMID:25173 | Effects of total fasting in obese women. II. Acid-base balance. | Acetoacetate, beta-hydroxybutyrate, pyruvate and lactate in whole blood, the acid-base balance according to Astrup, SGOT and SGPT, and the serum and urinary sodium and potassium were estimated in 10 fasting obese women. Fasting led metabolic acidosis due to a tenfold increase of acetoacetate and beta-hydroxybutyrate, while pyruvate and lactate had a tendency to decrease. Serum and urinary sodium and potassium decreased; A tendency to increased SGOT and SGPT was observed. Sodium bicarbonate administered in general since the 8th or 9th day of fasting led in 4 to 5 days to an earlier correction of blood pH, while pCO2, actual HCO3, standard HCO3, base excess, buffer base, pO2, Hb saturation and total CO2 reached the prefasting values only several days after blood pH normalisation. The administration of NaHCO3 corrected the serum level of sodium while its excretion remained low. Bicarbonate had no influence on the decreasing trend of serum potassium, but the urinary output of potassium was stabilized. It is suggested that in fasting subjects 1. the correction of the metabolic acidosis by NaHCO3 does not reduce the rate of lipid catabolism and does not lead to fluid retention and 2. liver damage in fasting may be prevented by blood pH normalisation. | Effects of total fasting in obese women. II. Acid-base balance. Acetoacetate, beta-hydroxybutyrate, pyruvate and lactate in whole blood, the acid-base balance according to Astrup, SGOT and SGPT, and the serum and urinary sodium and potassium were estimated in 10 fasting obese women. Fasting led metabolic acidosis due to a tenfold increase of acetoacetate and beta-hydroxybutyrate, while pyruvate and lactate had a tendency to decrease. Serum and urinary sodium and potassium decreased; A tendency to increased SGOT and SGPT was observed. Sodium bicarbonate administered in general since the 8th or 9th day of fasting led in 4 to 5 days to an earlier correction of blood pH, while pCO2, actual HCO3, standard HCO3, base excess, buffer base, pO2, Hb saturation and total CO2 reached the prefasting values only several days after blood pH normalisation. The administration of NaHCO3 corrected the serum level of sodium while its excretion remained low. Bicarbonate had no influence on the decreasing trend of serum potassium, but the urinary output of potassium was stabilized. It is suggested that in fasting subjects 1. the correction of the metabolic acidosis by NaHCO3 does not reduce the rate of lipid catabolism and does not lead to fluid retention and 2. liver damage in fasting may be prevented by blood pH normalisation. | [
-0.04820710048079491,
0.014092610217630863,
0.005376456771045923,
0.09106195718050003,
0.021967096254229546,
0.011314251460134983,
0.008183224126696587,
-0.020337849855422974,
-0.04838915541768074,
0.018312208354473114,
0.05746586620807648,
-0.09375011920928955,
0.011051698587834835,
0.04690118134021759,
0.0038763652555644512,
-0.035445719957351685,
0.011599311605095863,
-0.02626589871942997,
-0.07137822359800339,
0.046196646988391876,
-0.001315896981395781,
-0.0022653318010270596,
0.0027347379364073277,
0.007360646966844797,
0.03049115277826786,
-0.02656235173344612,
-0.012350087985396385,
-0.013535170815885067,
-0.06472812592983246,
-0.19007208943367004,
0.0346481092274189,
0.0006431379006244242,
0.014960645698010921,
-0.04533381015062332,
-0.04140714555978775,
0.050091177225112915,
-0.011823422275483608,
0.06664060801267624,
-0.05074477568268776,
0.025773407891392708,
0.05730864778161049,
0.028824593871831894,
-0.007536943536251783,
0.01872425526380539,
-0.01916935108602047,
0.020763449370861053,
-0.017223302274942398,
0.03666609898209572,
0.09350695461034775,
0.04388277605175972,
0.011365755461156368,
0.01961098052561283,
-0.01902133785188198,
0.10020573437213898,
0.014137398451566696,
-0.020818209275603294,
0.0022182706743478775,
0.022325871512293816,
-0.00008543747389921919,
-0.023105338215827942,
-0.040560055524110794,
0.039964985102415085,
-0.1498272866010666,
0.048337966203689575,
0.021058542653918266,
-0.006097403354942799,
-0.04844304919242859,
-0.03817809373140335,
0.022302281111478806,
0.0530315600335598,
-0.07486739754676819,
0.09111695736646652,
0.026989474892616272,
0.04295339435338974,
0.03422169387340546,
0.001977798994630575,
0.01911778934299946,
0.021443339064717293,
-0.03741221874952316,
0.023565884679555893,
-0.016803639009594917,
-0.019769297912716866,
0.016971945762634277,
0.014941957779228687,
0.03210853412747383,
-0.0759810134768486,
0.02537805400788784,
-0.000633485906291753,
0.00019237172091379762,
-0.005837827920913696,
-0.03019648790359497,
-0.0513596385717392,
-0.08069354295730591,
0.040235646069049835,
-0.016067035496234894,
0.03352789208292961,
-0.01563122496008873,
-0.054001521319150925,
-0.013377728872001171,
0.3930973410606384,
0.013799108564853668,
0.10573757439851761,
-0.03784792497754097,
0.010541488416492939,
0.03493322432041168,
-0.05770113319158554,
0.03265706077218056,
-0.005069015081971884,
-0.00245252950116992,
-0.0036542981397360563,
0.037158552557229996,
0.04479945823550224,
0.028502190485596657,
-0.04790367931127548,
-0.028677923604846,
0.040810760110616684,
0.07763293385505676,
0.009474714286625385,
0.00714616384357214,
0.040238793939352036,
-0.03396359831094742,
0.012322244234383106,
0.03211682662367821,
-0.018902618438005447,
0.028545446693897247,
0.019839560613036156,
0.03108065016567707,
0.06580279022455215,
0.013331945985555649,
0.06001584231853485,
0.06770968437194824,
-0.014200398698449135,
0.002267028670758009,
0.025704801082611084,
-0.04323597624897957,
-0.025523943826556206,
0.05107218399643898,
-0.03567531704902649,
0.06267008930444717,
0.009488156996667385,
-0.029836168512701988,
-0.055233679711818695,
-0.03472256287932396,
-0.1024361103773117,
-0.012683233246207237,
0.13264407217502594,
0.042570121586322784,
0.011595717631280422,
0.0012424384476616979,
-0.00833640806376934,
0.06013564392924309,
-0.02150782011449337,
-0.045761559158563614,
0.02827068604528904,
-0.027908124029636383,
0.004442048259079456,
0.006159652024507523,
0.016101565212011337,
-0.004282032139599323,
-0.07173467427492142,
-0.0804295614361763,
-0.041947875171899796,
-0.0010492471046745777,
-0.022526904940605164,
0.03258085250854492,
0.010488196276128292,
0.0015878027770668268,
-0.04906581714749336,
-0.01483227126300335,
-0.005837211385369301,
0.037478890269994736,
-0.0016562268137931824,
-0.014957872219383717,
-0.10410765558481216,
0.06606557965278625,
0.02362184040248394,
0.04353569447994232,
0.039225202053785324,
0.009483951143920422,
0.0014802555087953806,
0.05113052949309349,
0.02522139996290207,
-0.030959682539105415,
0.03317718580365181,
0.021864434704184532,
-0.09579665958881378,
-0.07991300523281097,
0.0016312674852088094,
0.01070277951657772,
0.031152235344052315,
-0.020507369190454483,
-0.02159726247191429,
-0.14424559473991394,
-0.052607256919145584,
-0.02345319092273712,
-0.07306744903326035,
-0.046738866716623306,
-0.00030376017093658447,
-0.010126551613211632,
0.032156795263290405,
0.061759911477565765,
0.04537743702530861,
0.007900062948465347,
0.011481408961117268,
0.026850968599319458,
0.017765896394848824,
-0.03802602365612984,
-0.020408762618899345,
0.018871666863560677,
0.034030720591545105,
0.001566635794006288,
0.05066473409533501,
-0.04106410592794418,
0.019219690933823586,
-0.033998411148786545,
0.019638588652014732,
0.04353208839893341,
-0.005317771341651678,
0.029360223561525345,
0.011952880769968033,
0.09216871857643127,
-0.06069706752896309,
-0.01778995618224144,
-0.2949967384338379,
-0.009695254266262054,
0.02931361459195614,
-0.011865857057273388,
0.048013534396886826,
-0.04184411093592644,
0.006171303801238537,
-0.022019295021891594,
-0.0010480493074283004,
-0.019781379029154778,
0.017992988228797913,
0.049787987023591995,
-0.0326874703168869,
-0.015209734439849854,
0.008834528736770153,
0.033180296421051025,
-0.0037344666197896004,
-0.0989069789648056,
0.055514223873615265,
0.010729902423918247,
0.0002637947036419064,
-0.03017394058406353,
0.003030566032975912,
0.00788198970258236,
0.10200648009777069,
0.052421558648347855,
0.06809074431657791,
-0.01502565760165453,
0.009403740987181664,
-0.015174460597336292,
0.014883358962833881,
0.018124938011169434,
0.028361067175865173,
-0.10366103053092957,
0.04563054069876671,
-0.05169246718287468,
-0.001652514562010765,
-0.0013827456859871745,
-0.04701237753033638,
-0.007662545423954725,
-0.1003316268324852,
-0.046491846442222595,
-0.02236761897802353,
-0.008139398880302906,
-0.05140458419919014,
0.03209858760237694,
0.01162000559270382,
-0.009057597257196903,
-0.007304255850613117,
-0.013992532156407833,
0.02623084746301174,
-0.001467268681153655,
-0.053165968507528305,
0.001712578465230763,
0.08139026165008545,
-0.024004682898521423,
-0.04741182178258896,
0.012037061154842377,
-0.09130961447954178,
-0.0209551602602005,
-0.012548791244626045,
-0.03836517408490181,
-0.0012156767770648003,
-0.027819013223052025,
0.005747713148593903,
-0.016768205910921097,
-0.0038143182173371315,
0.024679694324731827,
0.03393381088972092,
-0.002775028580799699,
0.0030468301847577095,
0.11146681010723114,
-0.04080492630600929,
0.002161127282306552,
0.05841992422938347,
0.02349204011261463,
-0.06102711334824562,
-0.05642150714993477,
-0.059116020798683167,
-0.03185805305838585,
-0.014890537597239017,
-0.04149935021996498,
0.08444932103157043,
0.011631332337856293,
0.0023248749785125256,
0.039579398930072784,
0.02931583859026432,
-0.01145514752715826,
0.0021466100588440895,
0.09553475677967072,
-0.0017949157627299428,
0.00941929966211319,
-0.029101422056555748,
-0.019480649381875992,
0.011299004778265953,
0.047791365534067154,
-0.2616886496543884,
0.029826655983924866,
-0.021214814856648445,
-0.026386462152004242,
0.0005719973705708981,
-0.009789466857910156,
-0.04131849855184555,
0.005528111010789871,
-0.036125097423791885,
0.04017815738916397,
-0.004169147927314043,
0.06081593781709671,
0.06423430889844894,
0.01740841753780842,
-0.016136351972818375,
0.018213896080851555,
0.05927078798413277,
0.012336082756519318,
0.020954886451363564,
-0.03501627594232559,
0.012644638307392597,
-0.033880267292261124,
0.11225350946187973,
0.03239716961979866,
-0.058090731501579285,
0.036732304841279984,
0.01444215513765812,
-0.011480466462671757,
-0.03695675730705261,
-0.006716189906001091,
-0.03145727142691612,
0.03496735543012619,
0.04915851354598999,
0.014594348147511482,
0.029620787128806114,
0.04325835406780243,
-0.0004946188419125974,
-0.024023177102208138,
0.004579995293170214,
0.030823227018117905,
0.04375612363219261,
-0.02735944464802742,
-0.02568504586815834,
-0.043668873608112335,
0.06824202835559845,
-0.04584456607699394,
-0.06051088869571686,
-0.05842984840273857,
0.0024858007673174143,
-0.01632091775536537,
-0.019667815417051315,
-0.06590051203966141,
0.04242539405822754,
0.04755998030304909,
0.031377021223306656,
-0.020430315285921097,
-0.04070707783102989,
-0.009956657886505127,
-0.01462678425014019,
-0.032077062875032425,
0.011004711501300335,
-0.0019229768076911569,
-0.03325260058045387,
0.04957819730043411,
0.002272028708830476
] |
PMID:25174 | Effect of substrate composition and concentration on aortic cholesterol ester hydrolase activity. | Cholesterol ester hydrolase activity of pig aorta has been examined under optimum experimental conditions for hydrolysis of different cholesterol esters. The enzyme specific activity values were in the numerical order of substrates hydrolyzed: cholesteryl linoleate larger than or equal to linolenate greater than palmitate larger than or equal to stearate greater than oleate. The results are discussed in relation to the arterial accumulation of cholesterol esters. | Effect of substrate composition and concentration on aortic cholesterol ester hydrolase activity. Cholesterol ester hydrolase activity of pig aorta has been examined under optimum experimental conditions for hydrolysis of different cholesterol esters. The enzyme specific activity values were in the numerical order of substrates hydrolyzed: cholesteryl linoleate larger than or equal to linolenate greater than palmitate larger than or equal to stearate greater than oleate. The results are discussed in relation to the arterial accumulation of cholesterol esters. | [
-0.03268144652247429,
0.011859199963510036,
-0.03976741433143616,
0.01848423108458519,
-0.0213051475584507,
-0.0295344740152359,
-0.028442377224564552,
0.06440446525812149,
0.025161856785416603,
-0.07188688963651657,
0.016681041568517685,
-0.05121958628296852,
-0.017537739127874374,
-0.027265841141343117,
-0.008185707032680511,
-0.05306810513138771,
0.07188595086336136,
0.02132929116487503,
-0.03927946463227272,
0.028330128639936447,
0.04522300139069557,
0.06542495638132095,
0.01336380373686552,
-0.04489549249410629,
0.019163785502314568,
0.012871098704636097,
0.011381657794117928,
-0.030499549582600594,
-0.06809598207473755,
-0.18587340414524078,
0.018002986907958984,
-0.05088689178228378,
0.026509128510951996,
-0.02210945449769497,
-0.012426797300577164,
0.0336991585791111,
-0.026255479082465172,
0.02381640300154686,
-0.02122514881193638,
0.02583482302725315,
0.07156378030776978,
-0.00015042921586427838,
-0.012727465480566025,
0.04512862488627434,
0.012661447748541832,
0.007853584364056587,
-0.05591969192028046,
-0.016055036336183548,
0.0552910640835762,
-0.012908496893942356,
0.016110604628920555,
0.014155938290059566,
0.006133733782917261,
-0.0006836703978478909,
-0.0027898920234292746,
-0.03593157231807709,
-0.005787752103060484,
0.03763746842741966,
0.011459309607744217,
-0.012226688675582409,
-0.021458877250552177,
0.03362162411212921,
-0.13705986738204956,
0.08587412536144257,
-0.026024192571640015,
0.03316804766654968,
0.0006978139863349497,
-0.03166830539703369,
0.04827670007944107,
0.02856503054499626,
-0.029407385736703873,
0.01953738182783127,
0.017127154394984245,
-0.029447538778185844,
-0.007198701612651348,
0.013750243932008743,
0.007970290258526802,
0.02130545862019062,
0.019982438534498215,
-0.010568727739155293,
0.006944030523300171,
0.02194032073020935,
0.01261055190116167,
-0.009619329124689102,
0.03143933042883873,
0.020973488688468933,
0.006348422262817621,
-0.027594929561018944,
0.01991475187242031,
0.003142546396702528,
-0.005190173629671335,
-0.05572867766022682,
-0.032049354165792465,
-0.03255045413970947,
-0.03596082702279091,
0.08987275511026382,
0.045689381659030914,
-0.02995925024151802,
-0.030886603519320488,
0.3918538987636566,
-0.0656559020280838,
-0.007271790411323309,
-0.049013324081897736,
-0.00048598949797451496,
-0.008989856578409672,
0.006822443567216396,
-0.07519548386335373,
0.018391380086541176,
-0.023557789623737335,
-0.01802586019039154,
0.09051381051540375,
0.009047077037394047,
-0.049983538687229156,
-0.05012592300772667,
-0.06028975918889046,
-0.015032337047159672,
0.005471411161124706,
0.02400348335504532,
-0.021201251074671745,
0.02348332852125168,
-0.0031025700736790895,
0.029663410037755966,
0.007346953731030226,
0.010784325189888477,
0.046307988464832306,
0.013173343613743782,
0.027801280841231346,
0.09317281097173691,
0.04676521196961403,
0.03377041965723038,
-0.007068756967782974,
0.02189825288951397,
-0.023811178281903267,
-0.03482436388731003,
0.01985872909426689,
0.005110039841383696,
0.08826946467161179,
-0.01113465242087841,
0.05874130502343178,
-0.01780984364449978,
0.03451941907405853,
-0.0026673562824726105,
-0.04444364085793495,
-0.07034976035356522,
-0.004199108108878136,
0.12133420258760452,
0.055511146783828735,
0.01464055571705103,
-0.04692411050200462,
0.03622322529554367,
-0.033864859491586685,
-0.0029933168552815914,
-0.04950641468167305,
0.10516498237848282,
-0.04847954213619232,
-0.01193156000226736,
-0.037035491317510605,
0.010799098759889603,
0.00798142421990633,
-0.033966630697250366,
-0.11291086673736572,
-0.023491138592362404,
-0.03515786677598953,
0.06432582437992096,
0.008757135830819607,
-0.006269460078328848,
-0.02560489997267723,
0.023159470409154892,
0.029023785144090652,
0.012515057809650898,
0.021109312772750854,
-0.034230902791023254,
-0.041957248002290726,
0.0047515034675598145,
-0.014591348357498646,
0.01890232041478157,
0.04828967526555061,
0.05937669426202774,
-0.007791366893798113,
0.008783522993326187,
0.02953183278441429,
-0.01077955774962902,
0.0013815539423376322,
0.031129375100135803,
0.04771508648991585,
-0.024520890787243843,
-0.04228109493851662,
-0.04750610515475273,
0.04021374136209488,
0.056055426597595215,
-0.029748855158686638,
-0.05973220616579056,
-0.07820969820022583,
-0.04272603616118431,
-0.02870514988899231,
-0.07222439348697662,
-0.02364582195878029,
0.09466022253036499,
0.011420763097703457,
0.05775170400738716,
0.10195810347795486,
0.0249791219830513,
0.006377461366355419,
0.04538864269852638,
-0.003623320022597909,
0.06886360049247742,
-0.008697171695530415,
-0.04278537258505821,
-0.0006563795614056289,
0.002538777654990554,
-0.055064089596271515,
-0.0058485944755375385,
-0.036596499383449554,
0.015570270828902721,
-0.04222146049141884,
0.03009430132806301,
0.04608762264251709,
-0.016721397638320923,
0.10292693972587585,
-0.03339890390634537,
0.03818736597895622,
-0.08022352308034897,
-0.0014066314324736595,
-0.2985842823982239,
-0.10169941931962967,
0.024901289492845535,
-0.006939501501619816,
0.06484147906303406,
-0.034179508686065674,
-0.021617505699396133,
-0.032754283398389816,
-0.03385124355554581,
-0.05123816058039665,
0.019658774137496948,
0.0009722775430418551,
-0.018372902646660805,
-0.028232751414179802,
0.04192240536212921,
0.054286323487758636,
-0.00301909982226789,
-0.05759434029459953,
0.016415251418948174,
-0.00587431900203228,
0.014344452880322933,
-0.04052800312638283,
-0.004577567335218191,
0.025679750367999077,
0.15543712675571442,
-0.014272036962211132,
0.08766748011112213,
-0.023823458701372147,
-0.03186720609664917,
0.006652537267655134,
0.040683548897504807,
0.012047454714775085,
-0.0069478717632591724,
-0.07328496873378754,
-0.03670715168118477,
-0.06585181504487991,
0.02676278166472912,
-0.07974505424499512,
0.025114860385656357,
0.02408413402736187,
-0.031102340668439865,
-0.0013288724003359675,
-0.011927708983421326,
-0.0697987973690033,
-0.06727217137813568,
-0.017167307436466217,
-0.01921013370156288,
0.054442547261714935,
-0.057315677404403687,
0.012419932521879673,
0.061176639050245285,
-0.011510656215250492,
0.018791930750012398,
0.024096012115478516,
0.04516326263546944,
-0.007209238596260548,
-0.025025298818945885,
0.003162676701322198,
-0.04125316068530083,
0.018153920769691467,
-0.026872437447309494,
-0.022984793409705162,
0.010099224746227264,
0.02414979785680771,
-0.02908475324511528,
0.057178616523742676,
0.023832596838474274,
-0.030527424067258835,
0.0034637232311069965,
0.01683248020708561,
-0.037957195192575455,
0.049892887473106384,
-0.0018083025934174657,
-0.036324188113212585,
0.03307725489139557,
0.02976323664188385,
-0.019890213385224342,
-0.06321512162685394,
-0.017458263784646988,
0.03945815563201904,
-0.007863199338316917,
-0.06462424993515015,
0.015202349983155727,
-0.0020478556398302317,
0.014416137710213661,
-0.0026341346092522144,
0.009459237568080425,
-0.04320970177650452,
-0.012061911635100842,
-0.0018295914633199573,
-0.0015449505299329758,
-0.03417634591460228,
-0.07389131188392639,
-0.0017346483655273914,
0.01428072340786457,
-0.04108190909028053,
-0.27027982473373413,
-0.02691388875246048,
0.04590076208114624,
0.011422710493206978,
0.014231032691895962,
-0.017449090257287025,
0.02233419567346573,
0.03465372696518898,
0.01363840140402317,
0.02383962646126747,
-0.004401395097374916,
0.00923884566873312,
0.01558038778603077,
0.05419301241636276,
0.035961542278528214,
0.038251668214797974,
0.03801035135984421,
-0.019794410094618797,
-0.006300689186900854,
-0.03426521643996239,
-0.013819878920912743,
-0.022923937067389488,
0.17462700605392456,
0.03507060185074806,
-0.04261019080877304,
0.03839908167719841,
0.028997523710131645,
0.029442686587572098,
-0.04651349037885666,
-0.005660227965563536,
-0.0000635591204627417,
-0.02498689852654934,
0.077084019780159,
-0.01291893795132637,
-0.012669653631746769,
0.09632053226232529,
0.006046614609658718,
-0.003730694530531764,
-0.026856059208512306,
0.05121791735291481,
0.07701415568590164,
-0.005761389154940844,
-0.1172165721654892,
-0.0018695155158638954,
0.04827459901571274,
-0.008963401429355145,
0.00614125607535243,
-0.01655500940978527,
-0.0033496839459985495,
0.002103513805195689,
-0.02976461872458458,
-0.06001682206988335,
-0.0031251967884600163,
0.0527387335896492,
0.04786074534058571,
-0.016606273129582405,
-0.01766042783856392,
0.014723692089319229,
-0.045186836272478104,
0.05133431404829025,
0.005924532189965248,
0.01999633014202118,
0.005213525611907244,
0.12259414792060852,
0.049616869539022446
] |
PMID:25175 | Butadiene diepoxide activation of guanylate cyclase. | A variety of nitroso chemical carcinogens increase the activity of guanylate cyclase (EC 4.6.1.2), the enzyme catalyzing the production of guanosine 3',5'-monophosphate. In the present report, the first non-nitroso chemical carcinogen, butadiene diepoxide, was shown to activate guanylate in a variety of tissues over the concentration range 1-100 mmol/l. At 20 mmol/l concentration, increases were 2- to 17-fold above control. These observations have potential importance since guanosine 3',5'-monophosphate may be involved in cell growth and malignant transformation. | Butadiene diepoxide activation of guanylate cyclase. A variety of nitroso chemical carcinogens increase the activity of guanylate cyclase (EC 4.6.1.2), the enzyme catalyzing the production of guanosine 3',5'-monophosphate. In the present report, the first non-nitroso chemical carcinogen, butadiene diepoxide, was shown to activate guanylate in a variety of tissues over the concentration range 1-100 mmol/l. At 20 mmol/l concentration, increases were 2- to 17-fold above control. These observations have potential importance since guanosine 3',5'-monophosphate may be involved in cell growth and malignant transformation. | [
-0.04807500168681145,
-0.016732921823859215,
-0.007848076522350311,
-0.018117673695087433,
0.011868413537740707,
-0.026077477261424065,
0.026460150256752968,
0.015952561050653458,
-0.04198189452290535,
-0.05781036615371704,
0.015926942229270935,
-0.052441149950027466,
-0.04849126935005188,
-0.005110913421958685,
-0.021763790398836136,
-0.0500323623418808,
0.0027196763549000025,
-0.0002142410958185792,
-0.06073204427957535,
0.009709561243653297,
-0.013797922991216183,
0.011035877279937267,
0.07055312395095825,
0.009517897851765156,
0.040447380393743515,
0.010606561787426472,
0.025719640776515007,
0.030675390735268593,
-0.017171069979667664,
-0.18868258595466614,
-0.01364076416939497,
0.014479545876383781,
-0.010719276033341885,
-0.04334212467074394,
-0.014659836888313293,
0.01575448177754879,
-0.023250192403793335,
-0.011813851073384285,
-0.04294436052441597,
0.01838388293981552,
0.014400920830667019,
0.01728767342865467,
-0.04329570382833481,
0.010954857803881168,
0.016868487000465393,
0.02256317250430584,
-0.029055403545498848,
-0.0066022793762385845,
0.06260126829147339,
-0.027397561818361282,
0.07293297350406647,
-0.05500685051083565,
-0.056884847581386566,
-0.0036440968979150057,
0.026942012831568718,
-0.04114465415477753,
0.07288262993097305,
0.012670557014644146,
-0.02021750621497631,
0.014456307515501976,
0.022843386977910995,
0.07732509821653366,
-0.14885453879833221,
0.0037171863950788975,
0.021372539922595024,
-0.0011509099276736379,
-0.03859356418251991,
-0.019683199003338814,
0.02547319233417511,
0.04098111018538475,
-0.03558092936873436,
0.04465148597955704,
0.0006078715086914599,
0.03316567465662956,
-0.013276972807943821,
0.02695605345070362,
-0.023783942684531212,
0.02781311422586441,
0.029923025518655777,
0.0003580442280508578,
0.012553412467241287,
0.009136516600847244,
0.02758248895406723,
-0.03952579200267792,
0.0036145413760095835,
-0.03108702041208744,
-0.09657205641269684,
-0.013848962262272835,
-0.017213869839906693,
0.03389047086238861,
-0.02552044950425625,
-0.03552385792136192,
0.03003084473311901,
-0.041063420474529266,
-0.021311648190021515,
0.00170545419678092,
0.08028221130371094,
-0.004150749649852514,
-0.008465767838060856,
0.329437792301178,
-0.061602022498846054,
-0.008646245114505291,
-0.09673236310482025,
0.00229740422219038,
-0.03719590976834297,
-0.012119886465370655,
-0.06956887245178223,
0.04366912320256233,
0.024745434522628784,
0.022404640913009644,
0.06633730232715607,
0.005652367603033781,
0.009607602842152119,
-0.07094201445579529,
-0.022595111280679703,
0.043286919593811035,
0.0846148431301117,
0.009607440792024136,
-0.03297186270356178,
0.12093713879585266,
0.0576603077352047,
-0.027535708621144295,
0.014271142892539501,
0.027282141149044037,
0.0014399307547137141,
0.029391979798674583,
-0.02673996053636074,
0.08046179264783859,
0.061962295323610306,
0.02123597450554371,
0.027718590572476387,
0.00669249938800931,
-0.016719112172722816,
0.011205367743968964,
0.002017043065279722,
-0.017891153693199158,
0.05467839539051056,
-0.007077058777213097,
-0.01937539502978325,
0.006699180696159601,
-0.019835736602544785,
-0.02050137147307396,
-0.0476861409842968,
-0.10041089355945587,
0.003566498402506113,
0.06553500145673752,
-0.008331858552992344,
0.05459699407219887,
-0.021532483398914337,
0.022308286279439926,
0.0358092337846756,
0.047215983271598816,
-0.06259769201278687,
0.02735874429345131,
-0.004153549671173096,
0.0085618169978261,
-0.02018721029162407,
0.0012775135692209005,
0.020727334544062614,
-0.001237422227859497,
-0.1047956794500351,
-0.007786198519170284,
0.03345755860209465,
0.06502220779657364,
0.06522815674543381,
-0.03790527582168579,
-0.07194744795560837,
0.019093764945864677,
0.01294214092195034,
0.020891085267066956,
0.03799203038215637,
0.001829243265092373,
-0.002043687039986253,
-0.022765817120671272,
0.052996207028627396,
0.06611128896474838,
0.061311934143304825,
0.01757495291531086,
0.04299790412187576,
0.03650202602148056,
0.07246524840593338,
-0.019805168733000755,
0.006569219287484884,
0.06022576987743378,
-0.013705425895750523,
-0.059822600334882736,
-0.04153774306178093,
0.0024564312770962715,
-0.0034757982939481735,
0.03599598631262779,
-0.0300598107278347,
0.003056613029912114,
-0.05474209785461426,
0.03156573697924614,
-0.06718993932008743,
-0.05902824550867081,
-0.028866492211818695,
-0.026767689734697342,
0.017860043793916702,
-0.03701899200677872,
0.07337559014558792,
-0.019129574298858643,
0.02766486443579197,
0.05772170051932335,
-0.0037845568731427193,
0.024892859160900116,
-0.018201280385255814,
0.07067932933568954,
0.03386694937944412,
-0.057314906269311905,
-0.016465600579977036,
0.015287412330508232,
-0.006559102330356836,
0.02736218087375164,
-0.0024892811197787523,
0.03604920580983162,
-0.020984262228012085,
0.02892426960170269,
0.07787702977657318,
-0.02501482330262661,
0.08102771639823914,
-0.06083064526319504,
-0.05803413316607475,
-0.3094111382961273,
-0.0946357399225235,
-0.02448279596865177,
-0.01480995211750269,
0.0367434099316597,
-0.03601667657494545,
0.03386300429701805,
-0.0422777496278286,
-0.009550405666232109,
-0.03963160514831543,
0.07787669450044632,
0.08385823667049408,
-0.004238484427332878,
0.0344952829182148,
-0.015901820734143257,
0.01586468145251274,
0.039691757410764694,
-0.060769274830818176,
0.006923651322722435,
0.01697496697306633,
0.01231984980404377,
0.00006559270696016029,
-0.05060100555419922,
0.008580431342124939,
0.09663216769695282,
-0.04630192741751671,
0.07260943949222565,
0.008753172121942043,
0.035080764442682266,
-0.05509720370173454,
0.06165667250752449,
-0.005000214092433453,
-0.055055171251297,
-0.06285835802555084,
-0.020032431930303574,
-0.047422681003808975,
-0.023703202605247498,
-0.018307369202375412,
-0.0546252503991127,
0.03191762790083885,
-0.019958095625042915,
-0.04482153430581093,
-0.021855855360627174,
0.0010405646171420813,
-0.006834888830780983,
-0.025841647759079933,
0.02635830081999302,
0.07114079594612122,
-0.007228674367070198,
-0.029901232570409775,
0.04614269360899925,
0.04214920103549957,
0.05821961537003517,
-0.024037159979343414,
-0.019444169476628304,
-0.010240919888019562,
-0.03596765920519829,
0.05412479117512703,
-0.02537834271788597,
-0.03761930763721466,
-0.0009345888975076377,
-0.025570178404450417,
-0.008446462452411652,
-0.0008954133954830468,
-0.013763374648988247,
-0.04369645565748215,
0.016426855698227882,
-0.017007235437631607,
0.08321315795183182,
0.05772298574447632,
-0.02190340869128704,
0.12939223647117615,
-0.06447429955005646,
0.0059875864535570145,
0.015924058854579926,
0.01644965074956417,
-0.043541934341192245,
-0.031062033027410507,
-0.06883493810892105,
-0.02562187798321247,
-0.02307932823896408,
0.003696120111271739,
0.03593318164348602,
0.012648770585656166,
-0.0003249074216000736,
0.05187016353011131,
0.018875839188694954,
-0.05050289258360863,
0.03840622678399086,
0.03336546570062637,
0.04993556812405586,
0.031348396092653275,
-0.006755371578037739,
-0.05587301030755043,
0.050801459699869156,
-0.013802126981317997,
-0.29233893752098083,
-0.06052109971642494,
-0.006913055200129747,
0.045742832124233246,
-0.0188940167427063,
0.00923279020935297,
0.03288979083299637,
-0.028855053707957268,
-0.03154286742210388,
0.09167728573083878,
-0.0029250429943203926,
0.00712656369432807,
-0.02332070656120777,
0.09430093318223953,
-0.04263924062252045,
0.009732388891279697,
0.0015076871495693922,
-0.03594803810119629,
-0.029076624661684036,
-0.004688525106757879,
0.03376568853855133,
-0.007061961572617292,
0.18916940689086914,
-0.026308197528123856,
-0.009060985408723354,
0.06935518980026245,
-0.06070510298013687,
-0.002269673626869917,
-0.054246094077825546,
0.04853275418281555,
-0.021833742037415504,
0.050202347338199615,
0.045008335262537,
-0.10203936696052551,
0.007886462844908237,
0.07159766554832458,
0.028673136606812477,
0.012014803476631641,
-0.02051559090614319,
-0.013753117062151432,
-0.05477016791701317,
0.007521532010287046,
-0.022877829149365425,
-0.03496522083878517,
0.06777969002723694,
-0.09090861678123474,
-0.02747972123324871,
-0.02343299612402916,
0.020824719220399857,
0.019011491909623146,
-0.011489481665194035,
0.014693604782223701,
0.014965005218982697,
0.009430707432329655,
-0.002733701141551137,
-0.03759133815765381,
-0.021984659135341644,
-0.03980906680226326,
0.03651197627186775,
0.05333016440272331,
-0.007685164455324411,
0.021086467429995537,
-0.010989218018949032,
0.0758526623249054,
0.05390067398548126
] |
PMID:25177 | Purification and characterization of the two molecular forms of membrane acid protease from Aspergillus oryzae. | Two forms (M1 and M2) of the membrane-bound acid protease of Aspergillus oryzae have been purified by extraction with Triton X-100, washing with cold acetone, and repeated gel filtration on Bio-Gel A-15 m in the presence and absence of Triton X-100. The purified membrane enzymes, M1 and M2, moved as a single band in acrylamide gel electrophoresis and had apparent molecular weights of 150 000 and 60 000, respectively, as estimated by sodium dodecyl sulfate/acrylamide gel electrophoresis. These two membrane enzymes activated bovine pancreatic trypsinogen and had the same pH optima in the acid pH range. They immunologically cross-reacted with each other and with an extracellular acid protease from A. oryzae, and contained carbohydrate, ranging from 52.5 to 80.5% and comprising three hexoses, glucose, galactose, and mannose. While these catalytic, chemical and immunological properties are similar to those of the extracellular acid protease from A. oryzae, both membrane enzyme differed in their hydrophobic properties from external enzymes. Thus they are activated by the detergent Triton X-100 and some polar lipids. | Purification and characterization of the two molecular forms of membrane acid protease from Aspergillus oryzae. Two forms (M1 and M2) of the membrane-bound acid protease of Aspergillus oryzae have been purified by extraction with Triton X-100, washing with cold acetone, and repeated gel filtration on Bio-Gel A-15 m in the presence and absence of Triton X-100. The purified membrane enzymes, M1 and M2, moved as a single band in acrylamide gel electrophoresis and had apparent molecular weights of 150 000 and 60 000, respectively, as estimated by sodium dodecyl sulfate/acrylamide gel electrophoresis. These two membrane enzymes activated bovine pancreatic trypsinogen and had the same pH optima in the acid pH range. They immunologically cross-reacted with each other and with an extracellular acid protease from A. oryzae, and contained carbohydrate, ranging from 52.5 to 80.5% and comprising three hexoses, glucose, galactose, and mannose. While these catalytic, chemical and immunological properties are similar to those of the extracellular acid protease from A. oryzae, both membrane enzyme differed in their hydrophobic properties from external enzymes. Thus they are activated by the detergent Triton X-100 and some polar lipids. | [
-0.08348793536424637,
-0.035864587873220444,
-0.04741576686501503,
0.0478864349424839,
-0.03645104542374611,
-0.08024557679891586,
0.06004774570465088,
0.07914548367261887,
-0.02941460721194744,
-0.021512523293495178,
-0.014367283321917057,
-0.09392207860946655,
-0.030622076243162155,
0.024321623146533966,
-0.018712308257818222,
0.0298630278557539,
0.008107457309961319,
0.008240894414484501,
-0.08003083616495132,
-0.03135193884372711,
0.03103608265519142,
-0.01756231300532818,
0.032158613204956055,
-0.04046999290585518,
0.08541291952133179,
0.04285058751702309,
-0.01336172129958868,
0.010530399158596992,
-0.06402238458395004,
-0.18091422319412231,
0.036796074360609055,
-0.032784756273031235,
0.04629996418952942,
-0.02340838313102722,
-0.012864586897194386,
0.05309809744358063,
-0.0008722812053747475,
-0.06302272528409958,
-0.04042720049619675,
0.01903672330081463,
0.04170944169163704,
0.06582161784172058,
-0.01224348321557045,
0.016679609194397926,
0.023218924179673195,
-0.011787005700170994,
-0.014585435390472412,
0.004955601878464222,
0.08181268721818924,
-0.007895703427493572,
0.008239030838012695,
0.032318390905857086,
0.0021485823672264814,
0.01682346500456333,
0.027853626757860184,
-0.05276566743850708,
0.01340901106595993,
0.03951932489871979,
-0.03786090016365051,
0.016652503982186317,
0.026783544570207596,
-0.016131319105625153,
-0.11203980445861816,
0.07379410415887833,
0.05855543538928032,
0.02009735070168972,
-0.049285467714071274,
-0.04491555318236351,
0.04204997420310974,
0.03010578267276287,
-0.06612780690193176,
0.058352258056402206,
-0.025250393897294998,
-0.003838292323052883,
0.015636363998055458,
0.014352160505950451,
-0.020802892744541168,
-0.0035354983992874622,
0.018322192132472992,
0.006860290188342333,
-0.01943635568022728,
-0.05283438414335251,
-0.015194807201623917,
0.061197951436042786,
0.016002174466848373,
0.03135039284825325,
-0.015964491292834282,
0.008944236673414707,
0.04067392274737358,
0.012359917163848877,
-0.009801177307963371,
-0.03474319726228714,
0.018584607169032097,
0.02828921750187874,
-0.08512198179960251,
0.014572708867490292,
0.0265661608427763,
-0.010716523043811321,
0.0326337106525898,
0.3710308372974396,
-0.001462992513552308,
-0.013394157402217388,
-0.023914823308587074,
-0.045546066015958786,
-0.027385378256440163,
-0.015393263660371304,
-0.048569802194833755,
0.01449767965823412,
-0.01529211550951004,
0.06215876340866089,
0.08636024594306946,
0.034594256430864334,
-0.009289921261370182,
-0.019340474158525467,
0.025902921333909035,
-0.003170812502503395,
0.02375487983226776,
-0.011480155400931835,
-0.01899617724120617,
0.08378485590219498,
-0.06104345992207527,
-0.03276781737804413,
-0.007723075337707996,
0.0065740374848246574,
0.031751058995723724,
0.046992722898721695,
-0.016762007027864456,
0.11031339317560196,
0.09562302380800247,
0.0006375744706019759,
0.0519087016582489,
0.04830292612314224,
-0.0330352857708931,
-0.0013344614999368787,
0.0045512220822274685,
-0.06027814745903015,
0.036109644919633865,
0.010005027987062931,
0.024570416659116745,
-0.04454684257507324,
-0.02497449330985546,
-0.02301868051290512,
-0.054942257702350616,
-0.048090483993291855,
-0.0005694504361599684,
0.030636807903647423,
-0.020024999976158142,
0.015702886506915092,
-0.04090181365609169,
0.025983735918998718,
0.011591501533985138,
0.07074229419231415,
0.00034337356919422746,
0.0451909601688385,
0.03747755289077759,
0.025913923978805542,
-0.0024904063902795315,
-0.04438500106334686,
0.0511334054172039,
-0.017006104812026024,
-0.06921597570180893,
0.015042399987578392,
-0.009115817956626415,
0.10070814192295074,
0.08932128548622131,
-0.032397352159023285,
-0.08994036167860031,
-0.02592039294540882,
0.016145208850502968,
0.006637549493461847,
0.007397402543574572,
0.006242475006729364,
-0.05248871073126793,
-0.030915601179003716,
-0.0804407075047493,
0.015836378559470177,
-0.020344335585832596,
0.00859939493238926,
0.002753621432930231,
0.01972595416009426,
0.012322109192609787,
0.0073448182083666325,
-0.03406798839569092,
-0.020772095769643784,
-0.0011274674907326698,
-0.04896307364106178,
-0.00012394986697472632,
-0.027820371091365814,
0.005746763199567795,
0.025798188522458076,
-0.023015812039375305,
0.02320285327732563,
-0.08686228096485138,
0.007383161224424839,
-0.039543718099594116,
-0.0334959551692009,
-0.046317461878061295,
-0.05452054738998413,
-0.02684355340898037,
-0.05516798049211502,
0.10363107919692993,
0.007915974594652653,
-0.020528368651866913,
0.05339023843407631,
-0.0029870918951928616,
0.04121499881148338,
0.020327461883425713,
0.05179879814386368,
0.03094351477921009,
0.036796070635318756,
-0.026017356663942337,
-0.008626733906567097,
-0.1147570013999939,
0.007357671856880188,
-0.04394223913550377,
0.07026712596416473,
0.025014154613018036,
0.022966675460338593,
0.03949644789099693,
0.03548908233642578,
-0.0037674359045922756,
-0.018810125067830086,
-0.046755775809288025,
-0.2893529236316681,
0.023307565599679947,
-0.05243203788995743,
0.012696508318185806,
0.04237769544124603,
-0.059121023863554,
-0.002384707797318697,
-0.06142708659172058,
-0.00397743983194232,
-0.02728414535522461,
0.06017574295401573,
0.05024368315935135,
0.021695774048566818,
-0.00424474012106657,
-0.010774540714919567,
0.04987229406833649,
0.008948588743805885,
-0.04822232201695442,
0.049117304384708405,
-0.0012846110621467233,
-0.003931296989321709,
-0.01769311912357807,
0.09292497485876083,
0.019044924527406693,
0.07208601385354996,
-0.07220035791397095,
0.08328568190336227,
0.000720827141776681,
-0.00892146211117506,
-0.0034417614806443453,
0.009357865899801254,
0.03659951686859131,
0.004794205538928509,
-0.05425761640071869,
-0.030225325375795364,
-0.07449616491794586,
0.023737790063023567,
-0.02869465947151184,
-0.007037429604679346,
-0.04615689441561699,
0.010705625638365746,
0.013707705773413181,
0.004084842745214701,
-0.0780486911535263,
-0.010835324414074421,
-0.0023806854151189327,
0.058562736958265305,
0.07035510987043381,
-0.01022438146173954,
0.04149743169546127,
0.036862265318632126,
0.009473214857280254,
0.014600031077861786,
-0.062021851539611816,
-0.04386008158326149,
0.001250133733265102,
0.007746036164462566,
0.033336054533720016,
-0.03783480077981949,
0.022284917533397675,
-0.05796542763710022,
-0.01827210746705532,
-0.037090953439474106,
0.033544812351465225,
-0.039816562086343765,
-0.011825465597212315,
-0.020550580695271492,
0.00007135239866329357,
0.030722489580512047,
0.011006725952029228,
0.04615157097578049,
0.08425174653530121,
-0.06211685389280319,
-0.03714318946003914,
0.03281773626804352,
0.05591987818479538,
0.0360199511051178,
-0.06790821254253387,
-0.04307654872536659,
0.037613287568092346,
0.004427430685609579,
-0.02935825288295746,
0.09123804420232773,
-0.0740370899438858,
0.04804926738142967,
0.04553614929318428,
-0.008237861096858978,
0.016494348645210266,
0.021935874596238136,
0.014475606381893158,
0.029799416661262512,
0.00032390590058639646,
-0.032556306570768356,
-0.0018960867309942842,
-0.007895434275269508,
-0.008025571703910828,
-0.271444171667099,
0.021042121574282646,
0.04709421098232269,
0.04720931872725487,
-0.0602233000099659,
0.040012188255786896,
0.03517146781086922,
-0.07363669574260712,
-0.036001384258270264,
-0.010684632696211338,
0.05554579943418503,
0.022490134462714195,
0.06699619442224503,
-0.005332329776138067,
-0.023047083988785744,
0.05352180451154709,
0.07207220792770386,
-0.05126862972974777,
0.0539395846426487,
-0.025212641805410385,
-0.019826265051960945,
-0.059402331709861755,
0.1385415494441986,
0.013075499795377254,
0.01378948986530304,
0.039526671171188354,
-0.01007848046720028,
0.0011787627590820193,
-0.03856819495558739,
0.014936045743525028,
0.05432531237602234,
0.05337730422616005,
0.061709094792604446,
0.005249180365353823,
-0.019970033317804337,
0.002567822113633156,
0.0028743133880198,
0.006221753545105457,
-0.06786048412322998,
0.037249863147735596,
-0.0067800795659422874,
-0.06850318610668182,
-0.1076510027050972,
-0.029071027413010597,
0.01292412355542183,
-0.08728242665529251,
-0.05615033954381943,
-0.015808040276169777,
-0.01181767974048853,
0.0038337695877999067,
-0.007342895492911339,
-0.01863079145550728,
0.08085095882415771,
0.027438370510935783,
0.023362834006547928,
-0.021999401971697807,
-0.027259288355708122,
0.017681540921330452,
-0.08907457441091537,
0.027384527027606964,
0.021408801898360252,
0.017475338652729988,
-0.013396534137427807,
0.029167965054512024,
0.03787163645029068
] |
PMID:25179 | Spectrofluorometric measurement of the binding of ethidium to superhelical DNA from cell nuclei. | Structures retaining many of the morphological features of nuclei may be released by lysing HeLa cells in solutions containing non-ionic detergents and high concentrations of salt. These nucleoids contain few chromatin proteins. We have shown that the DNA of nucleoids is quasicircular and supercoiled by measure spectrofluorometrically the amount of the intercalating dye, ethidium, bound to unirradiated and gamma-irradiated nucleoids. Ethidium binds to nucleoids in the manner characteristic of the binding to superhelical DNA: at low concentrations more ethidium binds to unirradiated nucleoids than to their gamma-irradiated counterparts with broken DNA, and at higher concentrations less ethidium binds to the unirradiated nucleoids. The quasi-circles in nucleoids are 22 times less sensitive to gamma-irradiation than are circles of pure PM2 DNA: they must contain about 2.2 X 10(5) base pairs. The constraints that maintain the quasi-circularity of nucleoid DNA are very resistant to extremes of temperature and alkali; some remain under conditions in which the duplex is denatured. The constraints are destabilised by ethidium suggesting that they are stabilised by free energy of supercoiling. Proteolytic enzymes, but not ribonucleases, remove the constraints. Possible structures for the constraining mechanism are discussed. | Spectrofluorometric measurement of the binding of ethidium to superhelical DNA from cell nuclei. Structures retaining many of the morphological features of nuclei may be released by lysing HeLa cells in solutions containing non-ionic detergents and high concentrations of salt. These nucleoids contain few chromatin proteins. We have shown that the DNA of nucleoids is quasicircular and supercoiled by measure spectrofluorometrically the amount of the intercalating dye, ethidium, bound to unirradiated and gamma-irradiated nucleoids. Ethidium binds to nucleoids in the manner characteristic of the binding to superhelical DNA: at low concentrations more ethidium binds to unirradiated nucleoids than to their gamma-irradiated counterparts with broken DNA, and at higher concentrations less ethidium binds to the unirradiated nucleoids. The quasi-circles in nucleoids are 22 times less sensitive to gamma-irradiation than are circles of pure PM2 DNA: they must contain about 2.2 X 10(5) base pairs. The constraints that maintain the quasi-circularity of nucleoid DNA are very resistant to extremes of temperature and alkali; some remain under conditions in which the duplex is denatured. The constraints are destabilised by ethidium suggesting that they are stabilised by free energy of supercoiling. Proteolytic enzymes, but not ribonucleases, remove the constraints. Possible structures for the constraining mechanism are discussed. | [
-0.01355532743036747,
-0.01037808507680893,
-0.005576188676059246,
-0.02120509184896946,
0.08226748555898666,
-0.007116776425391436,
0.04752696678042412,
-0.011038580909371376,
-0.05054168775677681,
-0.0008692695992067456,
0.035525958985090256,
-0.009273306466639042,
0.005459027364850044,
-0.012998467311263084,
-0.040562231093645096,
-0.014735030941665173,
-0.04972108080983162,
0.005834465380758047,
-0.04459204897284508,
0.0105437645688653,
0.051109667867422104,
-0.017376059666275978,
-0.0032375850714743137,
-0.016796348616480827,
0.008666763082146645,
0.07209005206823349,
-0.002982432022690773,
0.013802115805447102,
-0.02214828133583069,
-0.22180090844631195,
0.029024574905633926,
0.0023136737290769815,
-0.0027986352797597647,
-0.00007052221917547286,
0.0016964850947260857,
-0.010274807922542095,
-0.020090358331799507,
-0.06739526242017746,
-0.04623612388968468,
0.009828248992562294,
0.019464707002043724,
0.08309105783700943,
0.0037306726444512606,
-0.030579805374145508,
-0.013965355232357979,
0.03721993416547775,
0.0018827890744432807,
-0.01497227419167757,
0.02673647738993168,
0.016558418050408363,
-0.004711673129349947,
0.0006288393633440137,
-0.022274060174822807,
0.05495192110538483,
-0.02652798779308796,
0.03089699149131775,
0.0738101378083229,
0.052198052406311035,
0.019785063341259956,
0.04001158848404884,
-0.007828986272215843,
0.027821416035294533,
-0.07777359336614609,
0.04177772253751755,
0.08408670872449875,
-0.06606566160917282,
-0.02760469540953636,
-0.042023733258247375,
0.018534637987613678,
0.0617554672062397,
-0.0427926741540432,
0.08634867519140244,
-0.01744440756738186,
0.05539144575595856,
0.009671872481703758,
-0.017577409744262695,
-0.015565554611384869,
-0.05801151692867279,
0.01398869976401329,
-0.01201534178107977,
-0.009448207914829254,
0.0025405914057046175,
-0.0016781189478933811,
-0.008757319301366806,
-0.031008735299110413,
0.046965088695287704,
-0.05461101233959198,
-0.018291130661964417,
-0.016556376591324806,
0.03829024359583855,
-0.004222942981868982,
-0.032270487397909164,
0.008930183947086334,
-0.002267268253490329,
-0.03985849767923355,
0.005934393033385277,
0.0185539647936821,
0.03155521675944328,
0.0398462750017643,
0.4012211859226227,
-0.07594908028841019,
0.04384485259652138,
-0.0504259318113327,
0.0031415997073054314,
-0.03065337985754013,
-0.011106855235993862,
-0.0030572533141821623,
-0.038157105445861816,
-0.028230225667357445,
-0.010178272612392902,
0.062197573482990265,
-0.011214165948331356,
0.032942041754722595,
-0.056316226720809937,
0.05713231861591339,
-0.007319134660065174,
-0.021548045799136162,
-0.013501179404556751,
-0.05159909278154373,
-0.0222726259380579,
-0.035801906138658524,
-0.020877337083220482,
0.02581319585442543,
-0.031737469136714935,
0.08545664697885513,
0.0018933196552097797,
-0.07614848762750626,
0.05023937299847603,
0.018728045746684074,
0.002560035791248083,
0.05151880532503128,
0.006967292632907629,
-0.017984656617045403,
0.002930351998656988,
-0.0032821502536535263,
0.002665042644366622,
0.019494127482175827,
-0.03555496037006378,
0.038076918572187424,
0.01764613203704357,
0.009797804988920689,
-0.11267897486686707,
-0.052406083792448044,
-0.08447214215993881,
-0.02659979648888111,
0.0885981023311615,
-0.03575502708554268,
0.04041053354740143,
-0.022230293601751328,
0.03157741203904152,
-0.005297188647091389,
0.04396624490618706,
-0.00866716355085373,
0.011099402792751789,
-0.009329142048954964,
0.0482618473470211,
-0.007169983349740505,
0.030109426006674767,
-0.027159539982676506,
0.008450728841125965,
-0.048435863107442856,
0.06511692702770233,
0.010512326844036579,
0.07199221849441528,
0.022759046405553818,
-0.049182791262865067,
-0.01604004204273224,
0.024398356676101685,
0.019732216373085976,
0.01058262214064598,
0.008425178937613964,
-0.017917750403285027,
0.026688050478696823,
-0.03392675146460533,
-0.04443743824958801,
0.04375312104821205,
-0.013707855716347694,
-0.014721079729497433,
-0.015857046470046043,
0.02749945968389511,
-0.0019871762488037348,
-0.014722988940775394,
0.0005338620976544917,
0.026366129517555237,
0.029289240017533302,
0.010428772307932377,
-0.056141700595617294,
0.014998550526797771,
-0.028899548575282097,
0.0247945673763752,
0.010278970934450626,
0.0572778657078743,
-0.03520585969090462,
0.03491075709462166,
-0.06844302266836166,
-0.07906343042850494,
0.016955479979515076,
0.04104648530483246,
-0.03976874798536301,
-0.013048119843006134,
0.12531939148902893,
-0.001337474095635116,
0.002483910880982876,
0.09385287016630173,
0.05936567485332489,
-0.0047681452706456184,
0.0118433041498065,
-0.005191711243242025,
0.06017757207155228,
-0.009836052544414997,
-0.023881159722805023,
-0.03460583835840225,
-0.04171450063586235,
0.023248735815286636,
-0.09778821468353271,
0.04102404788136482,
0.05842242389917374,
-0.023715782910585403,
-0.001268325257115066,
-0.0014875223860144615,
0.04979662597179413,
-0.09688415378332138,
-0.07730133831501007,
-0.29661884903907776,
-0.059439465403556824,
-0.08928640186786652,
-0.01677834615111351,
0.023966297507286072,
0.026926301419734955,
0.06491416692733765,
-0.007060068193823099,
0.00407837750390172,
-0.09368913620710373,
0.008177857846021652,
0.023258551955223083,
0.02243667282164097,
-0.13150554895401,
-0.05791574344038963,
-0.013121289201080799,
0.031431980431079865,
0.002053134609013796,
0.029327305033802986,
0.04460577666759491,
-0.002613640855997801,
0.01345440000295639,
-0.09972931444644928,
0.056944407522678375,
0.05161970108747482,
-0.06268007308244705,
0.13994517922401428,
0.012775222770869732,
-0.03583807870745659,
0.009810516610741615,
-0.016575805842876434,
0.043663498014211655,
-0.009779850952327251,
-0.008408020250499249,
-0.033570267260074615,
-0.025396931916475296,
-0.06114228442311287,
-0.020624082535505295,
0.011593763716518879,
0.028905291110277176,
0.020976457744836807,
0.020403459668159485,
0.009027902968227863,
-0.05866304039955139,
-0.017780844122171402,
-0.024073436856269836,
-0.00315842661075294,
-0.005741029046475887,
0.008234427310526371,
0.011861560866236687,
0.04879618436098099,
-0.02498347871005535,
-0.025470657274127007,
-0.04285239428281784,
-0.0037603366654366255,
-0.00402423832565546,
0.0073777479119598866,
0.03560803830623627,
0.01764577627182007,
-0.010073752142488956,
-0.007175573613494635,
0.019956078380346298,
0.01606673374772072,
0.021992012858390808,
-0.018546350300312042,
-0.02309717983007431,
0.015974970534443855,
0.0015377311501652002,
0.058866821229457855,
-0.05588996037840843,
0.047011565417051315,
0.12460292875766754,
-0.061206668615341187,
0.045536041259765625,
0.021512823179364204,
0.03747990354895592,
-0.01810963824391365,
-0.022149914875626564,
-0.022258758544921875,
0.0403350368142128,
0.06455342471599579,
0.010603184811770916,
0.0905807688832283,
-0.04518717899918556,
-0.04052415117621422,
0.02478284016251564,
0.037474825978279114,
-0.014078606851398945,
0.030153464525938034,
0.009157024323940277,
0.009943710640072823,
-0.022122802212834358,
0.013150263577699661,
0.005830441601574421,
0.018980570137500763,
0.028790730983018875,
-0.28064611554145813,
-0.009534871205687523,
-0.024519182741642,
0.025775570422410965,
-0.055280186235904694,
0.020893359556794167,
0.07344333082437515,
-0.03926738724112511,
-0.03577154874801636,
0.036877695471048355,
0.02065453864634037,
0.03397194668650627,
0.0697721466422081,
0.0057164267636835575,
-0.010201089084148407,
0.03179283067584038,
0.05289038270711899,
-0.04962490499019623,
0.008637674152851105,
-0.009991717524826527,
-0.013858243823051453,
-0.019749531522393227,
0.1881846934556961,
-0.030375683680176735,
0.002566056791692972,
0.05057385191321373,
-0.011355207301676273,
0.04562531039118767,
0.0014915900537744164,
-0.010327356867492199,
-0.0033922982402145863,
0.04233192652463913,
0.03304440528154373,
-0.03514038771390915,
-0.06454034149646759,
0.0241515152156353,
0.004381374455988407,
-0.010438947938382626,
0.00486786337569356,
-0.05340025573968887,
0.010891035199165344,
0.028131119906902313,
-0.050577081739902496,
-0.048501309007406235,
0.05580073222517967,
-0.05774907395243645,
-0.03551473096013069,
-0.012635906226933002,
0.03518231585621834,
-0.004448202904313803,
-0.05792919546365738,
0.026508191600441933,
-0.000487973156850785,
0.01610429957509041,
-0.008411641232669353,
-0.018867764621973038,
-0.0305494237691164,
-0.02099606767296791,
0.0102030448615551,
-0.003607745748013258,
0.0033576786518096924,
-0.0078118243254721165,
-0.035261690616607666,
0.0778820812702179,
0.026708222925662994
] |
PMID:25181 | Characterization of beta-glucosidase isoenzymes possibly involved in lignification from chick pea (Cicer arietinum L.) cell suspension cultures. | Crude cell wall preparations from Cicer arietinum L. cell suspension cultures show high activity for the hydrolysis of coniferyl alcohol beta-D-glucoside (coniferin). Various beta-glucosidase activities could be solubilized from these preparations by 0.5 M NaCl treatment and one of these could be shown to possess a high activity for the hydrolysis of coniferin. The enzyme activities were purified to near homogeneity by Sephadex G-200 and CM-Sephadex chromatography. Isoelectric focussing indicated the occurrence of beta-glucosidase isoenzymes with identical catalytic activity (pI 8.5-10). Molecular weights were determined as 110 000, with two subunits of 63 000 and 43 000. Maximum hydrolytic activity is at pH 5. The beta-glucosidase isoenzymes catalyze the hydrolysis of various beta-glucosides with aromatic aglycone structure and different sugar moieties. However, coniferin has been found to be one of the best substrates (km = 0.8 mM; V = 6 mumol.min-1.mg protein-1) for these beta-glucosidase isoenzymes. The data suggest that beta-glucosidase-catalyzed reaction might be involved in lignification of these plant cell cultures. | Characterization of beta-glucosidase isoenzymes possibly involved in lignification from chick pea (Cicer arietinum L.) cell suspension cultures. Crude cell wall preparations from Cicer arietinum L. cell suspension cultures show high activity for the hydrolysis of coniferyl alcohol beta-D-glucoside (coniferin). Various beta-glucosidase activities could be solubilized from these preparations by 0.5 M NaCl treatment and one of these could be shown to possess a high activity for the hydrolysis of coniferin. The enzyme activities were purified to near homogeneity by Sephadex G-200 and CM-Sephadex chromatography. Isoelectric focussing indicated the occurrence of beta-glucosidase isoenzymes with identical catalytic activity (pI 8.5-10). Molecular weights were determined as 110 000, with two subunits of 63 000 and 43 000. Maximum hydrolytic activity is at pH 5. The beta-glucosidase isoenzymes catalyze the hydrolysis of various beta-glucosides with aromatic aglycone structure and different sugar moieties. However, coniferin has been found to be one of the best substrates (km = 0.8 mM; V = 6 mumol.min-1.mg protein-1) for these beta-glucosidase isoenzymes. The data suggest that beta-glucosidase-catalyzed reaction might be involved in lignification of these plant cell cultures. | [
0.004725100006908178,
-0.03219610080122948,
-0.0713692158460617,
-0.037231896072626114,
0.06698336452245712,
-0.03594436123967171,
-0.014186815358698368,
0.040341172367334366,
-0.038323428481817245,
0.017837489023804665,
-0.024094190448522568,
-0.08421112596988678,
-0.03617110103368759,
0.00345157110132277,
-0.01018773764371872,
-0.01438012532889843,
0.07078641653060913,
0.04803717881441116,
-0.060126736760139465,
-0.03020293451845646,
0.04292674735188484,
-0.05105702206492424,
0.026460299268364906,
-0.006074017379432917,
0.08095525950193405,
0.003434289013966918,
-0.04479258880019188,
-0.016123846173286438,
-0.08835645020008087,
-0.17285285890102386,
-0.05280847102403641,
-0.003648544894531369,
0.023609628900885582,
-0.013970051892101765,
0.033270854502916336,
0.04504586011171341,
-0.011548793874680996,
0.019451361149549484,
0.018181994557380676,
0.01954573020339012,
0.050033777952194214,
-0.0036080710124224424,
-0.04326147213578224,
0.04659142717719078,
0.043134696781635284,
-0.05290967598557472,
-0.057391244918107986,
-0.01838800311088562,
0.01940079592168331,
0.03295273333787918,
-0.0034361532889306545,
-0.0051087127067148685,
-0.002234792336821556,
-0.02611798606812954,
-0.01384373102337122,
0.028963014483451843,
0.024994952604174614,
0.039687253534793854,
0.0029598602559417486,
0.03129100799560547,
0.041887443512678146,
-0.030857834964990616,
-0.1469174027442932,
0.07396914064884186,
-0.022983409464359283,
0.019658101722598076,
-0.016352960839867592,
-0.02188662253320217,
0.09483087062835693,
0.09427674859762192,
-0.0499054379761219,
0.0754181370139122,
-0.014173808507621288,
0.018571950495243073,
0.020746223628520966,
-0.012941906228661537,
-0.022573674097657204,
0.047463610768318176,
0.022337408736348152,
0.02495555952191353,
0.017979227006435394,
-0.003885892918333411,
0.0067594884894788265,
-0.026740049943327904,
-0.02242892049252987,
0.008581679314374924,
0.03081420250236988,
0.00484630698338151,
0.01899765431880951,
0.02919158525764942,
-0.022189131006598473,
-0.050543639808893204,
-0.10177946835756302,
0.00964394025504589,
-0.08983676880598068,
0.0886451005935669,
0.0378648042678833,
0.013856879435479641,
0.0016910484991967678,
0.36006930470466614,
-0.024180617183446884,
-0.0049493154510855675,
-0.03991222754120827,
0.011822779662907124,
-0.013659336604177952,
-0.005752268247306347,
-0.04420866072177887,
-0.028844330459833145,
-0.007381746545433998,
0.006732391659170389,
0.06561824679374695,
-0.0371827594935894,
0.006796329282224178,
-0.014749929308891296,
-0.033803291618824005,
-0.04451582208275795,
0.016133280470967293,
-0.027573151513934135,
-0.06829509139060974,
0.07071498781442642,
0.031425293534994125,
-0.004964969586580992,
0.035493478178977966,
-0.03983968496322632,
0.09106966108083725,
0.018249008804559708,
-0.0368344821035862,
0.06927428394556046,
0.10256090015172958,
-0.005829575005918741,
0.058423109352588654,
0.034936483949422836,
0.04452703893184662,
-0.02089582569897175,
0.009760533459484577,
-0.035258881747722626,
0.05032826215028763,
0.018171636387705803,
-0.001572754350490868,
0.012101533822715282,
-0.03945554792881012,
-0.024326028302311897,
-0.010362925007939339,
-0.030529838055372238,
-0.01674349419772625,
0.049634017050266266,
0.01706952415406704,
0.015065674670040607,
0.011729174293577671,
0.0013810073724016547,
0.03429798036813736,
0.041047967970371246,
-0.002918601967394352,
0.020924320444464684,
0.02144559472799301,
-0.018971886485815048,
0.03076961264014244,
-0.024722870439291,
0.012560642324388027,
0.020728515461087227,
-0.048691004514694214,
0.03561514988541603,
0.04309427738189697,
0.06616918742656708,
0.04500456526875496,
-0.03705234453082085,
-0.043927859514951706,
0.0381615124642849,
0.02362773008644581,
-0.01013893261551857,
0.006917876657098532,
-0.019860295578837395,
0.0212730523198843,
-0.05996030196547508,
-0.02527809888124466,
0.02217589132487774,
0.025339927524328232,
0.00009811452764552087,
0.011351151391863823,
-0.0041617280803620815,
0.011868619360029697,
-0.027958516031503677,
0.005615827161818743,
0.022112399339675903,
0.08326737582683563,
-0.059818971902132034,
0.02934710495173931,
0.0088350223377347,
0.0013973481254652143,
0.05530613660812378,
-0.06838960945606232,
0.010447253473103046,
-0.11173129826784134,
0.019627628847956657,
-0.008763301186263561,
-0.07403566688299179,
-0.023239923641085625,
-0.051245447248220444,
0.04247744381427765,
-0.04438851401209831,
0.034168001264333725,
0.006342577748000622,
0.004023648798465729,
0.023851092904806137,
0.007592145353555679,
0.06284788995981216,
-0.025155024603009224,
0.017502330243587494,
0.020579738542437553,
0.0241224505007267,
-0.053007930517196655,
0.017336217686533928,
-0.0220403540879488,
-0.03402615711092949,
-0.01732940785586834,
0.07078327983617783,
0.016151489689946175,
0.012636573053896427,
0.04437996819615364,
0.03205800801515579,
0.024819672107696533,
-0.08928284794092178,
-0.07203660905361176,
-0.32430243492126465,
-0.08029935508966446,
0.028262276202440262,
-0.06319329887628555,
0.022325899451971054,
-0.041799016296863556,
-0.011730410158634186,
-0.02943316660821438,
0.0008233130793087184,
-0.08183541148900986,
0.05833093822002411,
0.05484137311577797,
0.025658851489424706,
-0.05009819194674492,
0.04469477757811546,
0.026380158960819244,
0.019447876140475273,
-0.03164702281355858,
0.05631297454237938,
-0.010218686424195766,
0.020510494709014893,
-0.08198802173137665,
0.08377491682767868,
0.010340409353375435,
0.038439083844423294,
-0.055369313806295395,
0.09474580734968185,
0.006337571423500776,
0.04468690603971481,
0.0033913461957126856,
-0.028910188004374504,
0.06064963340759277,
0.012052977457642555,
-0.07243561744689941,
0.012206364423036575,
-0.04700585827231407,
-0.025786934420466423,
-0.0012654219754040241,
0.04861651360988617,
0.022374244406819344,
-0.0128776291385293,
-0.005927671678364277,
0.01040789857506752,
-0.06568646430969238,
0.003768115770071745,
0.015413250774145126,
0.042770568281412125,
0.009139842353761196,
0.011854556389153004,
-0.008218075148761272,
0.00869238842278719,
0.005385735537856817,
0.047812994569540024,
0.03627120703458786,
0.011180821806192398,
0.042859870940446854,
-0.05647224560379982,
0.042209137231111526,
-0.06198164075613022,
0.005479515064507723,
-0.017624631524086,
-0.037506356835365295,
-0.021824710071086884,
0.08354262262582779,
0.022987645119428635,
-0.009396431967616081,
-0.0418606735765934,
-0.009102870710194111,
0.0434441864490509,
0.051579512655735016,
-0.0010936404578387737,
0.026366937905550003,
-0.0639711394906044,
-0.026461565867066383,
0.049784447997808456,
0.026394452899694443,
-0.04808640852570534,
-0.055610787123441696,
-0.06536713987588882,
-0.026515277102589607,
-0.017231829464435577,
-0.0790502056479454,
0.08457113057374954,
-0.021363142877817154,
-0.015129986219108105,
0.02915145270526409,
-0.008727138862013817,
-0.03496389836072922,
0.04150713235139847,
-0.01116760540753603,
0.038376420736312866,
0.004603707697242498,
-0.022118495777249336,
-0.034115903079509735,
0.07417372614145279,
-0.021845584735274315,
-0.22027699649333954,
-0.005384070798754692,
0.04335669428110123,
-0.021109389141201973,
0.039354681968688965,
0.009242808446288109,
0.04798091948032379,
-0.0010111979208886623,
0.015599820762872696,
0.04783572256565094,
0.015584656037390232,
0.023724228143692017,
0.045163530856370926,
0.011298025958240032,
-0.014023800380527973,
0.027757054194808006,
0.05318063497543335,
-0.03805892542004585,
0.06654445081949234,
-0.057052746415138245,
-0.016371287405490875,
-0.024489637464284897,
0.13624420762062073,
0.03549742326140404,
-0.002036208054050803,
0.06093336269259453,
-0.042833998799324036,
-0.03907577693462372,
-0.007929699495434761,
0.05591770261526108,
-0.015624219551682472,
-0.010234908200800419,
0.13713575899600983,
-0.0783734992146492,
-0.009931785054504871,
0.08623150736093521,
0.0003960471076425165,
-0.02998407930135727,
0.015237229876220226,
-0.04066489264369011,
0.01638486608862877,
-0.026501886546611786,
-0.11144737154245377,
-0.029441792517900467,
0.013558771461248398,
-0.08716026693582535,
-0.05664800852537155,
-0.07388049364089966,
0.0027771175373345613,
-0.00887337327003479,
-0.035329367965459824,
-0.027135714888572693,
-0.03390747308731079,
0.024428576231002808,
-0.02155865915119648,
-0.022644340991973877,
-0.08401618152856827,
0.030145471915602684,
-0.056439585983753204,
0.003962013870477676,
0.02073098160326481,
-0.0002716552116908133,
-0.03390376642346382,
0.09477944672107697,
0.01579020917415619
] |
PMID:25186 | Intrinsic sympathomimetic activity of beta-adrenoceptor blocking agents. | The pharmacological methods used to assess the intrinsic sympathomimetic activity (ISA) of beta-blockers are discussed. The clinical relevance of ISA to respiratory function, peripheral resistance and cardiac function is reviewed. It appears doubtful whether ISA is always of predominant clinical significance and an alternative explanation is offered for many clinical effects observed with certain beta-blockers, e.g. pindolol, oxprenolol, tolamolol, metoprolol, etc. Some effects of these beta-blockers resemble those of labetalol, a new drug with both alpha and beta-blocking activity. Some clinical effects of certain beta-blockers are more likely to be due to alpha-blocking activity than to their ISA. | Intrinsic sympathomimetic activity of beta-adrenoceptor blocking agents. The pharmacological methods used to assess the intrinsic sympathomimetic activity (ISA) of beta-blockers are discussed. The clinical relevance of ISA to respiratory function, peripheral resistance and cardiac function is reviewed. It appears doubtful whether ISA is always of predominant clinical significance and an alternative explanation is offered for many clinical effects observed with certain beta-blockers, e.g. pindolol, oxprenolol, tolamolol, metoprolol, etc. Some effects of these beta-blockers resemble those of labetalol, a new drug with both alpha and beta-blocking activity. Some clinical effects of certain beta-blockers are more likely to be due to alpha-blocking activity than to their ISA. | [
-0.03164353221654892,
-0.0007947853882797062,
0.012824353761970997,
0.0023272642865777016,
0.013179469853639603,
-0.022349001839756966,
0.06471569091081619,
-0.02112259902060032,
0.01997283846139908,
-0.0709058865904808,
-0.024908024817705154,
-0.006298460997641087,
-0.0073387897573411465,
0.07505874335765839,
-0.012519585900008678,
0.013043930754065514,
0.04840042442083359,
-0.03847810626029968,
0.008241541683673859,
-0.008949169889092445,
-0.0026963395066559315,
0.013962718658149242,
-0.00013351980305742472,
-0.0034572805743664503,
0.026600876823067665,
0.05936446413397789,
0.005609932355582714,
0.009879890829324722,
-0.05860280990600586,
-0.15747912228107452,
0.03471050783991814,
-0.016442004591226578,
0.03389957547187805,
0.03421657904982567,
-0.018467163667082787,
0.02873718924820423,
-0.03945370763540268,
0.08293486386537552,
-0.046726323664188385,
0.08171583712100983,
0.017743941396474838,
-0.022151077166199684,
-0.015641486272215843,
0.0719284936785698,
0.003472602926194668,
-0.0024915931280702353,
-0.050838638097047806,
-0.00369678670540452,
0.050549883395433426,
0.00748769287019968,
0.03691451996564865,
-0.017549913376569748,
0.004198605660349131,
0.07159535586833954,
0.02528741955757141,
-0.04448447376489639,
-0.03367128223180771,
0.04284409433603287,
0.07280448079109192,
0.00448367465287447,
-0.020261062309145927,
0.04524115100502968,
-0.15656109154224396,
0.019920842722058296,
0.0408918596804142,
0.04858594760298729,
-0.03526471555233002,
0.013433868065476418,
0.047228675335645676,
0.049316488206386566,
-0.023149479180574417,
0.03905525431036949,
0.012724684551358223,
0.00034563118242658675,
0.024283330887556076,
0.009488271549344063,
-0.01780257187783718,
-0.018249548971652985,
-0.029339823871850967,
0.011329535394906998,
0.0301572997123003,
0.0014453768962994218,
-0.046045202761888504,
-0.030037609860301018,
0.05520905926823616,
-0.007925092242658138,
0.06522898375988007,
-0.02869343012571335,
-0.029728693887591362,
0.017926935106515884,
-0.05758218467235565,
-0.060851722955703735,
-0.06138107553124428,
0.00533116701990366,
0.02046138234436512,
0.004229599144309759,
-0.053055290132761,
-0.012268342077732086,
-0.0449710339307785,
0.3561871647834778,
0.020239682868123055,
0.04500596970319748,
-0.10084044188261032,
0.014523627236485481,
0.03172793611884117,
-0.010451740585267544,
0.011847312562167645,
-0.040181174874305725,
-0.03134935349225998,
0.03253950551152229,
0.034373827278614044,
-0.02995510771870613,
0.07801588624715805,
-0.08057781308889389,
-0.0373581200838089,
-0.026547256857156754,
0.027079537510871887,
0.0060475911013782024,
0.010895006358623505,
-0.0001645320444367826,
-0.005667149554938078,
-0.018651701509952545,
0.027073709294199944,
0.0173919890075922,
0.07237366586923599,
-0.027717281132936478,
0.06526590138673782,
0.06636226922273636,
0.04561297222971916,
0.03937290608882904,
0.07282526046037674,
0.007265021093189716,
-0.031367987394332886,
-0.01143998559564352,
0.05359802767634392,
-0.04036404937505722,
0.024039149284362793,
0.006729581858962774,
0.033355116844177246,
-0.010727258399128914,
-0.0030680005438625813,
-0.025570277124643326,
0.052220240235328674,
-0.07314665615558624,
-0.07438775897026062,
0.1443033367395401,
0.024477142840623856,
0.00025583154638297856,
0.007099613081663847,
0.026158053427934647,
-0.053664304316043854,
0.03360652178525925,
-0.03243281692266464,
0.06783933192491531,
-0.06016465276479721,
-0.014889644458889961,
0.018398210406303406,
0.041617851704359055,
-0.029785973951220512,
-0.0538824126124382,
-0.1292165368795395,
-0.009968919679522514,
-0.021853452548384666,
0.09919582307338715,
-0.04587491601705551,
-0.008160791359841824,
-0.03845598176121712,
0.025384504348039627,
0.0038724562618881464,
0.022333096712827682,
-0.0022458257153630257,
-0.03943298012018204,
0.021437272429466248,
-0.053305163979530334,
-0.03165798634290695,
0.02735723741352558,
0.06772395223379135,
-0.05548536032438278,
0.042127445340156555,
-0.051569871604442596,
0.04434727504849434,
-0.030921485275030136,
0.03471813350915909,
-0.06960354000329971,
0.023149192333221436,
0.030437622219324112,
-0.050030775368213654,
-0.05044225603342056,
0.017700383439660072,
0.007353360299021006,
-0.030329421162605286,
-0.030586235225200653,
-0.07772888988256454,
-0.027241498231887817,
-0.010768896900117397,
-0.008695248514413834,
0.00960848294198513,
0.03638859838247299,
-0.07957550138235092,
-0.010804122313857079,
0.05691546946763992,
0.048069801181554794,
0.01450569462031126,
0.0036196194123476744,
-0.008916889317333698,
0.013275169767439365,
0.0032497330103069544,
-0.02182219736278057,
0.055798906832933426,
0.006815718486905098,
-0.010753589682281017,
0.016322890296578407,
0.01597357913851738,
0.021611573174595833,
-0.06684945523738861,
0.016415899619460106,
-0.025607693940401077,
0.009608983993530273,
0.030391937121748924,
0.006506495177745819,
-0.01162932813167572,
0.013162816874682903,
-0.004709398839622736,
-0.3131176233291626,
-0.044411126524209976,
0.01406022161245346,
-0.029404541477560997,
-0.018200349062681198,
-0.04212118312716484,
0.023194445297122,
-0.0216230358928442,
-0.04333130642771721,
-0.05393866077065468,
0.06618355214595795,
0.05442669987678528,
-0.044239193201065063,
-0.014839488081634045,
-0.022432804107666016,
0.06435581296682358,
0.02369951643049717,
-0.04162168875336647,
-0.003303499659523368,
0.012810853309929371,
-0.026143990457057953,
-0.01611381769180298,
-0.02254786528646946,
0.013248084113001823,
0.04244502633810043,
-0.014781822450459003,
0.07744897156953812,
-0.001570432330481708,
0.006812244653701782,
0.000907405570615083,
-0.0244474858045578,
0.04997667297720909,
0.02731414884328842,
-0.0810055211186409,
0.018044035881757736,
-0.0397941991686821,
0.08019254356622696,
-0.010684794746339321,
0.049052439630031586,
0.039516348391771317,
-0.08703866600990295,
0.029527202248573303,
-0.006572708021849394,
0.005761304870247841,
-0.05704997852444649,
0.05051768198609352,
0.06911365687847137,
0.013204914517700672,
-0.07125493884086609,
0.009037884883582592,
-0.02379755675792694,
-0.024645449593663216,
-0.00123903201892972,
-0.01977018266916275,
0.058082688599824905,
-0.008137006312608719,
-0.05025740712881088,
-0.05467308312654495,
-0.019945187494158745,
-0.014112983830273151,
-0.0000487793586216867,
0.06031887233257294,
0.05748777464032173,
-0.00043669468141160905,
0.006072940304875374,
-0.006617655511945486,
0.019595308229327202,
-0.019284892827272415,
0.06165962293744087,
-0.035570092499256134,
-0.002110706176608801,
0.11377929896116257,
-0.05882147699594498,
-0.03115972876548767,
0.03881984204053879,
-0.02062581665813923,
-0.0319049097597599,
-0.017716193571686745,
0.007238020189106464,
-0.0037375332321971655,
-0.026173941791057587,
0.016315359622240067,
0.02106275036931038,
-0.038454215973615646,
-0.027046775445342064,
-0.008526325225830078,
0.01849697157740593,
0.05211001634597778,
0.04806055128574371,
-0.032654713839292526,
-0.05316371098160744,
0.05553380027413368,
-0.0037816220428794622,
-0.03627057373523712,
0.051308419555425644,
-0.01649637334048748,
-0.2665846049785614,
-0.02332933433353901,
0.0424896776676178,
-0.012844144366681576,
-0.03435869887471199,
-0.042472757399082184,
0.02995298244059086,
-0.005221693776547909,
-0.09524904191493988,
-0.0076624187640845776,
-0.018327465280890465,
0.03282167762517929,
0.05168512463569641,
0.07446903735399246,
-0.03236951678991318,
-0.02119496464729309,
0.03580528497695923,
0.02644362859427929,
0.07985895872116089,
-0.09726480394601822,
0.008215020410716534,
0.024031903594732285,
0.15266776084899902,
0.023012936115264893,
-0.033016305416822433,
0.08283983170986176,
0.0164846982806921,
0.04869919642806053,
-0.06342314928770065,
-0.04255206882953644,
0.0492652952671051,
-0.07131946831941605,
0.06108647957444191,
-0.0035254249814897776,
0.038204364478588104,
0.02194192446768284,
-0.048747967928647995,
0.012932682409882545,
0.010446489788591862,
-0.04025155305862427,
-0.00614647613838315,
0.02201727405190468,
-0.056990738958120346,
-0.03129799664020538,
0.0840613842010498,
-0.05642919987440109,
-0.09805511683225632,
0.04441344738006592,
0.011947942897677422,
0.056212037801742554,
-0.020042167976498604,
-0.020105283707380295,
0.013734816573560238,
0.01928294450044632,
0.05467627942562103,
-0.016519159078598022,
-0.06508252024650574,
-0.003928830847144127,
-0.003171321004629135,
-0.023305343464016914,
-0.038546837866306305,
0.01216704398393631,
-0.025828789919614792,
0.0743812620639801,
0.04096626117825508
] |
PMID:25188 | Effect of diazepam on the frog neuromuscular junction. | Diazepam (10(-5) M) had no effect on the compound action potential of frog sciatic nerve and its refractory period. At the neuromuscular junction, no changes were found in the amplitude and frequency of miniature endplate potentials in the presence of the drug. End-plate potential (e.p.p.) amplitude and early facilitation of the e.p.p. were unchanged. Diazepam had no effect on the amplitude of potentials evoked by ACh applied iontophoretically (0.1 Hz) at low frequencies in the chemosensitive region of the postjunctional membrane. Diazepam (10(-6) M) enhanced the progressive desensitization to ACh applied at a frequency of 1 Hz but did not affect recovery of sensitivity. A possible mechanism of action of diazepam at cholinergic synapses is discussed. | Effect of diazepam on the frog neuromuscular junction. Diazepam (10(-5) M) had no effect on the compound action potential of frog sciatic nerve and its refractory period. At the neuromuscular junction, no changes were found in the amplitude and frequency of miniature endplate potentials in the presence of the drug. End-plate potential (e.p.p.) amplitude and early facilitation of the e.p.p. were unchanged. Diazepam had no effect on the amplitude of potentials evoked by ACh applied iontophoretically (0.1 Hz) at low frequencies in the chemosensitive region of the postjunctional membrane. Diazepam (10(-6) M) enhanced the progressive desensitization to ACh applied at a frequency of 1 Hz but did not affect recovery of sensitivity. A possible mechanism of action of diazepam at cholinergic synapses is discussed. | [
-0.020223643630743027,
-0.03876179829239845,
0.06752964109182358,
0.03489787504076958,
-0.024166500195860863,
-0.022959893569350243,
0.06255241483449936,
0.06604322791099548,
-0.03258442506194115,
-0.07383491098880768,
-0.00331883504986763,
-0.016990119591355324,
-0.004722632933408022,
0.07953014969825745,
0.013604779727756977,
0.0165729820728302,
0.0016007012454792857,
-0.04158240556716919,
-0.027767105028033257,
0.016367098316550255,
-0.03521627187728882,
0.035602863878011703,
0.03253066912293434,
0.030466169118881226,
-0.04466063529253006,
-0.03805713728070259,
0.009920130483806133,
0.03095949999988079,
-0.0311430711299181,
-0.17508506774902344,
0.024356510490179062,
0.011642851866781712,
-0.019885972142219543,
-0.057818975299596786,
-0.058601949363946915,
-0.0026485081762075424,
-0.01647666096687317,
-0.02229691855609417,
-0.021807227283716202,
0.020527131855487823,
0.0923549085855484,
0.027293168008327484,
-0.013889833353459835,
-0.02258116379380226,
-0.021860282868146896,
-0.03359644487500191,
0.007496288046240807,
-0.06163705140352249,
0.08663488924503326,
-0.054665885865688324,
0.04232586547732353,
-0.03211968019604683,
0.0930037721991539,
0.03607754036784172,
0.017691850662231445,
-0.008801735937595367,
-0.003609225619584322,
-0.013403655029833317,
-0.041137922555208206,
0.025590797886252403,
0.025688225403428078,
0.082761250436306,
-0.1396443098783493,
0.017984289675951004,
0.03237072750926018,
-0.001103698741644621,
0.022975122556090355,
0.01502526830881834,
0.008760012686252594,
0.051423732191324234,
-0.06899914145469666,
0.04380657151341438,
-0.030167700722813606,
-0.02229086495935917,
0.03939026594161987,
-0.01819976046681404,
-0.004430253989994526,
0.009048906154930592,
0.007862582802772522,
-0.07851586490869522,
-0.010160794481635094,
-0.01784377358853817,
0.021595634520053864,
-0.06111755967140198,
0.04628530144691467,
0.019053200259804726,
0.08711078763008118,
0.0031949009280651808,
-0.04217691347002983,
0.007516133598983288,
0.040443602949380875,
-0.061817191541194916,
-0.055477965623140335,
-0.0776868388056755,
-0.012339059263467789,
0.04120611399412155,
0.008798742666840553,
-0.002619786188006401,
-0.04323287680745125,
0.3524268865585327,
-0.03548377752304077,
0.025780722498893738,
-0.061830341815948486,
-0.03177134692668915,
-0.0020068641752004623,
-0.04079161211848259,
-0.00005614796464215033,
0.002755617257207632,
-0.03947698697447777,
0.03623994439840317,
0.07394616305828094,
-0.03509608283638954,
0.03111557848751545,
0.03862842917442322,
-0.046291183680295944,
0.0342656746506691,
0.06096605211496353,
0.007427865639328957,
0.05438755452632904,
0.012118514627218246,
-0.030495963990688324,
-0.0054695685394108295,
-0.015394587069749832,
-0.03391944244503975,
0.0824194848537445,
0.011701134033501148,
-0.09879143536090851,
0.11162405461072922,
0.029632331803441048,
0.015871847048401833,
0.06691695004701614,
-0.05265906825661659,
0.07644400745630264,
0.038458261638879776,
0.029881643131375313,
0.022811736911535263,
0.03575349971652031,
-0.04005032032728195,
-0.02748974785208702,
-0.046279095113277435,
0.0037416128907352686,
-0.07759741693735123,
-0.02595054917037487,
-0.0849308967590332,
-0.05063400790095329,
0.12615737318992615,
0.023327939212322235,
-0.023119403049349785,
-0.055639736354351044,
0.06173058971762657,
-0.000761402421630919,
0.033343587070703506,
-0.038491956889629364,
0.04336928576231003,
-0.0790320411324501,
0.05418410524725914,
-0.020271698012948036,
0.01711810566484928,
-0.004371362272650003,
-0.026753650978207588,
-0.07919157296419144,
-0.020420491695404053,
0.042402587831020355,
0.05720909312367439,
0.02020876109600067,
0.01728912815451622,
-0.051201432943344116,
-0.019048860296607018,
-0.05208892002701759,
-0.003919239621609449,
-0.033987756818532944,
0.025279425084590912,
-0.0468425415456295,
-0.0388287678360939,
-0.002873965073376894,
0.030068414285779,
0.058148112148046494,
-0.05659212917089462,
0.05544161796569824,
-0.0006415655370801687,
0.009483509697020054,
-0.004800559487193823,
0.012247650884091854,
-0.03474026918411255,
0.012628168798983097,
0.030051402747631073,
-0.0690101832151413,
0.004440744873136282,
0.005781211890280247,
0.03545978292822838,
-0.06397724896669388,
-0.05833848938345909,
-0.04235166683793068,
0.01574466936290264,
-0.039183273911476135,
-0.07716211676597595,
-0.03392963856458664,
-0.02019948698580265,
-0.015392327681183815,
-0.019913049414753914,
0.081368088722229,
0.04483579099178314,
-0.06267920136451721,
0.041928086429834366,
-0.02036912553012371,
0.04698215425014496,
-0.03156614303588867,
0.004945878870785236,
0.04983001574873924,
0.02192951738834381,
-0.029243113473057747,
-0.007954221218824387,
0.04742554947733879,
-0.03095397539436817,
-0.02204490639269352,
0.005190509371459484,
-0.08343522250652313,
0.08439115434885025,
0.029663117602467537,
0.027125589549541473,
0.027921361848711967,
-0.0611346997320652,
-0.0043906657956540585,
-0.30814996361732483,
-0.04167172685265541,
0.02015024796128273,
0.002466092584654689,
0.10475750267505646,
-0.02749590016901493,
0.06747256219387054,
-0.02809927612543106,
-0.008346932008862495,
-0.09937266260385513,
-0.005460858810693026,
0.0427103154361248,
-0.03903743252158165,
-0.013880493119359016,
0.012289381586015224,
0.016287539154291153,
0.032258763909339905,
-0.05260125920176506,
-0.008544917218387127,
0.007928768172860146,
-0.004535984713584185,
0.017362765967845917,
-0.019160181283950806,
0.06980562955141068,
0.022796999663114548,
-0.032004792243242264,
0.05004547908902168,
0.013558528386056423,
-0.00045730569399893284,
-0.0499064065515995,
-0.005558727774769068,
0.06805301457643509,
0.010948031209409237,
-0.09039926528930664,
0.014085663482546806,
0.03194436803460121,
-0.002213239436969161,
0.049062635749578476,
0.019049501046538353,
-0.04302611202001572,
-0.12337883561849594,
0.011953266337513924,
-0.0032402025535702705,
-0.04336480051279068,
-0.0658048465847969,
-0.021124061197042465,
0.01051410660147667,
-0.05751030147075653,
-0.06455516070127487,
-0.001430885517038405,
0.025218335911631584,
0.05395299941301346,
-0.020517999306321144,
-0.017322158440947533,
0.0584150105714798,
-0.0056761205196380615,
-0.02748950757086277,
0.04341535642743111,
-0.018975188955664635,
0.011525356210768223,
0.05071863532066345,
0.04951460286974907,
0.008611065335571766,
-0.03804192692041397,
-0.021569538861513138,
0.03769754618406296,
-0.007427705451846123,
0.004300902597606182,
0.07912303507328033,
0.016846826300024986,
0.017604602500796318,
0.14982640743255615,
0.0077952975407242775,
0.009891916066408157,
0.06443795561790466,
-0.03303322196006775,
-0.03245927765965462,
-0.03256947919726372,
-0.047861434519290924,
0.04441552981734276,
-0.03520488739013672,
-0.03870429843664169,
0.048593711107969284,
-0.031608931720256805,
-0.04767819494009018,
0.03663882613182068,
0.04749613627791405,
-0.044199954718351364,
0.05895770341157913,
0.054513655602931976,
-0.00027335743652656674,
0.01641702651977539,
-0.016182707622647285,
0.012031666934490204,
-0.0017065785359591246,
0.045185063034296036,
-0.24059683084487915,
-0.01457078754901886,
0.03580867499113083,
0.013415631838142872,
-0.012345835566520691,
0.027746250852942467,
0.054023005068302155,
-0.031322624534368515,
-0.05892104655504227,
0.014227043837308884,
-0.023061230778694153,
-0.008301344700157642,
0.008563677780330181,
0.052747104316949844,
-0.024699456989765167,
0.04375087097287178,
0.06055999919772148,
-0.02875501662492752,
0.03902231156826019,
0.005036535672843456,
0.03669465705752373,
0.02423791028559208,
0.1199335977435112,
0.05217287316918373,
0.026911098510026932,
0.023271270096302032,
-0.0011079575633630157,
-0.002893873257562518,
0.009035791270434856,
-0.0016159485094249249,
-0.009170398116111755,
0.016890570521354675,
0.03234756737947464,
-0.05961362272500992,
0.05594228580594063,
0.0051114861853420734,
-0.02759077027440071,
0.0028280860278755426,
-0.004068415146321058,
-0.043277960270643234,
0.02237841486930847,
0.05081431567668915,
-0.0167202390730381,
-0.0009956827852874994,
0.07230865210294724,
-0.05746212229132652,
-0.04494740068912506,
-0.03037358820438385,
0.02752910740673542,
0.05923089012503624,
-0.03774818778038025,
-0.01782480999827385,
0.04506603628396988,
0.010482985526323318,
0.01403301302343607,
-0.025182893499732018,
-0.026571102440357208,
0.014732086099684238,
0.03169837221503258,
-0.0193391814827919,
-0.007834572345018387,
-0.014795014634728432,
-0.038564249873161316,
0.05289294570684433,
0.027819206938147545
] |
PMID:25190 | Kinetics of blockade of different receptors by chlorpromazine in rabbit stomach strips. | The kinetics of receptor blockade by chlorpromazine (5 X 10(-8) M) were studied on fundic strips from the rabbit stomach. Onset of blockade by chlorpromazine proceeds at nearly identical rates when carbamylcholine and histamine are the agonists (t1/2 = approximately 5 min) while occurring more slowly against phenylephrine (t1/2 = 41 min). Similarly, offset of blockade by chlorpromazine is faster against carbamycholine and histamine (t1/2 = 83 and 34 min, respectively) than against phenylephrine (t1/2 = 385 min). The pA2 values of chlorpromazine on the alpha-adrenergic, muscarinic and histaminic (H1) receptors are 8.96, 7.17, and 7.90, respectively. No correlation exists between the kinetics of blockade and the affinity of chlorpromazine for the receptors. It is suggested, therefore, that alpha-adrenoreceptors in the rabbit stomach are less accessible to chlorpromazine, or distributed differently within the tissue, than either the muscarinic or histaminic receptors. | Kinetics of blockade of different receptors by chlorpromazine in rabbit stomach strips. The kinetics of receptor blockade by chlorpromazine (5 X 10(-8) M) were studied on fundic strips from the rabbit stomach. Onset of blockade by chlorpromazine proceeds at nearly identical rates when carbamylcholine and histamine are the agonists (t1/2 = approximately 5 min) while occurring more slowly against phenylephrine (t1/2 = 41 min). Similarly, offset of blockade by chlorpromazine is faster against carbamycholine and histamine (t1/2 = 83 and 34 min, respectively) than against phenylephrine (t1/2 = 385 min). The pA2 values of chlorpromazine on the alpha-adrenergic, muscarinic and histaminic (H1) receptors are 8.96, 7.17, and 7.90, respectively. No correlation exists between the kinetics of blockade and the affinity of chlorpromazine for the receptors. It is suggested, therefore, that alpha-adrenoreceptors in the rabbit stomach are less accessible to chlorpromazine, or distributed differently within the tissue, than either the muscarinic or histaminic receptors. | [
-0.07913238555192947,
-0.01946159452199936,
0.0029109877068549395,
0.07725854218006134,
-0.011498741805553436,
0.0025156913325190544,
0.0718589797616005,
0.006568896118551493,
-0.02157246135175228,
-0.03822943568229675,
0.013419483788311481,
-0.016995927318930626,
0.004497540183365345,
0.0550779327750206,
-0.006568780168890953,
0.0005147806950844824,
0.012515315786004066,
-0.010815858840942383,
-0.05582047253847122,
-0.02221750281751156,
-0.06924150884151459,
-0.036594096571207047,
0.008999191224575043,
-0.0063383495435118675,
0.031329263001680374,
0.013707121834158897,
-0.023079458624124527,
-0.03489728271961212,
-0.0343729704618454,
-0.20552414655685425,
0.04829417169094086,
-0.02637515962123871,
0.015905337408185005,
-0.012135285884141922,
-0.02942136488854885,
-0.019770542159676552,
-0.008950472809374332,
0.06104559451341629,
-0.0008972461218945682,
0.004131414461880922,
0.03155742585659027,
0.01690744422376156,
-0.008753077127039433,
-0.006824025418609381,
0.03118390217423439,
0.021847467869520187,
-0.03840009495615959,
0.012027936056256294,
0.080592580139637,
-0.017470816150307655,
0.020975438877940178,
0.015687983483076096,
0.0016618934459984303,
0.0200919508934021,
0.00936668086796999,
-0.030316008254885674,
-0.00037573176086880267,
0.025212841108441353,
-0.01370676327496767,
0.004887077957391739,
-0.025794068351387978,
0.0336197130382061,
-0.16812095046043396,
0.05371565371751785,
0.07049847394227982,
-0.0480082742869854,
0.020183946937322617,
-0.03318110480904579,
0.0274534672498703,
0.09451120346784592,
-0.055476114153862,
0.024099823087453842,
-0.03649183362722397,
-0.0035002182703465223,
-0.023025160655379295,
-0.07997573167085648,
0.00234087067656219,
0.015781797468662262,
0.040390100330114365,
0.0005113407387398183,
-0.017745213583111763,
0.02832873910665512,
-0.04318874329328537,
-0.038547333329916,
0.030027881264686584,
-0.0010249912738800049,
0.038502633571624756,
0.017769431695342064,
-0.0025126738473773003,
-0.00754943210631609,
-0.03984487056732178,
-0.08259927481412888,
-0.003423860063776374,
-0.03244912251830101,
-0.03176794573664665,
-0.012654885649681091,
-0.021715499460697174,
-0.01671425811946392,
-0.017806483432650566,
0.35693538188934326,
-0.017207572236657143,
0.0037569364067167044,
-0.027401261031627655,
0.023612773045897484,
0.01569686271250248,
0.030394449830055237,
0.0033217151649296284,
-0.010877984575927258,
-0.03838016837835312,
0.0209930669516325,
0.06912300735712051,
0.016791686415672302,
0.06479743868112564,
-0.09736799448728561,
0.004339037928730249,
0.0019355177646502852,
0.053966354578733444,
0.03402216359972954,
0.04243747517466545,
0.05739731714129448,
-0.0030025532469153404,
-0.04427121579647064,
-0.0003261029487475753,
0.016302505508065224,
0.011256680823862553,
0.030286483466625214,
-0.07523463666439056,
0.09821273386478424,
0.03285576030611992,
0.01771293766796589,
0.06108524650335312,
0.05502849072217941,
0.002416384406387806,
0.05993261933326721,
-0.0016109850257635117,
-0.012698006816208363,
0.06025740131735802,
-0.018354158848524094,
0.04166771098971367,
-0.06237790361046791,
-0.011947773396968842,
-0.09993405640125275,
-0.0009903530590236187,
-0.0971917137503624,
-0.08413092792034149,
0.0995899960398674,
0.0009145428775809705,
-0.01950487680733204,
-0.08083382248878479,
0.028363849967718124,
-0.04137750715017319,
0.005875652655959129,
0.026650115847587585,
0.04457807168364525,
-0.04665282741189003,
0.07265926897525787,
-0.04266082122921944,
0.026207391172647476,
-0.005361414514482021,
0.012172483839094639,
-0.06943344324827194,
-0.013818822801113129,
0.008634477853775024,
0.03453870117664337,
-0.015483144670724869,
0.011231283657252789,
0.0007080762879922986,
-0.02173939161002636,
0.00028323623700998724,
0.05401073396205902,
0.0006371281924657524,
0.05002458393573761,
-0.042107611894607544,
0.00911743938922882,
-0.057746004313230515,
-0.02531099133193493,
-0.014033435843884945,
-0.013582433573901653,
0.058340225368738174,
-0.008134805597364902,
0.026291368529200554,
0.032530639320611954,
0.006474785506725311,
-0.005752258468419313,
0.03097751922905445,
0.018126806244254112,
-0.04015669226646423,
0.029206952080130577,
-0.024423211812973022,
0.050411105155944824,
-0.02479381114244461,
-0.025814751163125038,
-0.061002761125564575,
0.01380467414855957,
-0.04843834787607193,
-0.042003996670246124,
-0.031496260315179825,
0.05551469326019287,
-0.07379729300737381,
-0.038232382386922836,
0.11657053232192993,
0.03664087504148483,
-0.027181332930922508,
0.0291458610445261,
-0.006861422676593065,
-0.008662012405693531,
0.009718875400722027,
-0.003440526081249118,
0.11075615137815475,
-0.028328435495495796,
-0.01676923781633377,
-0.005258269142359495,
0.0006386776803992689,
-0.04190576821565628,
-0.06161537021398544,
0.027183663100004196,
-0.019302407279610634,
0.017062697559595108,
0.04273848980665207,
0.053959380835294724,
0.018244843930006027,
0.005475000012665987,
-0.0034388615749776363,
-0.2865357995033264,
-0.03854414075613022,
0.018158450722694397,
0.03159080818295479,
0.07844790816307068,
-0.025589942932128906,
0.036192577332258224,
-0.0523994043469429,
-0.0409812331199646,
-0.06268507987260818,
0.016665931791067123,
0.045945752412080765,
-0.046052418649196625,
-0.003917600028216839,
0.005292948335409164,
0.06568396836519241,
0.05008712783455849,
0.04095596447587013,
0.05614098161458969,
0.00914226658642292,
0.025605885311961174,
-0.06003173440694809,
-0.048904214054346085,
0.03637073561549187,
0.013722172006964684,
0.029271509498357773,
0.10418123751878738,
0.015730995684862137,
-0.007326015271246433,
-0.010899421758949757,
0.020218800753355026,
-0.007657900452613831,
-0.04073430597782135,
-0.09047126024961472,
-0.04665079340338707,
-0.02873368188738823,
-0.0005852884496562183,
-0.04541883245110512,
0.05675886943936348,
-0.010366774164140224,
-0.07266073673963547,
0.06021447852253914,
0.059076257050037384,
-0.04971393942832947,
-0.07834695279598236,
0.036237772554159164,
-0.017193352803587914,
0.03909711539745331,
-0.010323869064450264,
-0.07324434816837311,
-0.0023436155170202255,
0.046168215572834015,
-0.0018087029457092285,
-0.03500444069504738,
0.008234278298914433,
0.010193542577326298,
-0.018352976068854332,
0.027766570448875427,
-0.07590454071760178,
0.04851117357611656,
-0.0045061721466481686,
0.023593876510858536,
0.05443114787340164,
-0.008296972140669823,
-0.0238008052110672,
0.01453570555895567,
0.030737143009901047,
0.01276986114680767,
-0.00008490880281897262,
0.015835464000701904,
0.061857424676418304,
0.10771742463111877,
-0.07194902747869492,
0.022234724834561348,
0.08528651297092438,
-0.029356960207223892,
0.03640451282262802,
0.004746406804770231,
-0.10838554799556732,
0.027751440182328224,
0.02495231106877327,
0.0033172317780554295,
0.014501878060400486,
0.016423678025603294,
-0.017771288752555847,
-0.043813399970531464,
0.01824909821152687,
-0.012162268161773682,
0.02958538942039013,
-0.003069179365411401,
-0.01003569271415472,
0.0395064502954483,
-0.07000367343425751,
-0.041321732103824615,
0.002559929620474577,
0.03287280350923538,
-0.27825579047203064,
0.04988803714513779,
0.05341753363609314,
0.023852713406085968,
0.007880748249590397,
-0.010113891214132309,
0.03626798093318939,
-0.050169650465250015,
-0.07822497934103012,
-0.050248101353645325,
-0.011818001978099346,
0.0364200659096241,
0.05325894057750702,
0.07284403592348099,
-0.035469900816679,
-0.0008706847438588738,
0.04202652350068092,
-0.05520957335829735,
-0.022502247244119644,
-0.019452130421996117,
-0.0019341423176229,
-0.023369822651147842,
0.1269165277481079,
0.028728483244776726,
-0.0005480038234964013,
0.07336507737636566,
0.028181293979287148,
0.030546216294169426,
0.06454876810312271,
-0.006956229452043772,
-0.018292834982275963,
0.03196929767727852,
0.0490005724132061,
-0.038963571190834045,
0.02636832930147648,
0.05790451169013977,
-0.08277619630098343,
0.007090103346854448,
-0.023115603253245354,
0.003820165991783142,
-0.032106656581163406,
0.04942789673805237,
-0.011447039432823658,
-0.008304152637720108,
0.06278735399246216,
-0.09896776080131531,
-0.04269774630665779,
-0.05020289868116379,
-0.012462488375604153,
0.050356242805719376,
-0.008708002977073193,
-0.04978901520371437,
-0.011087910272181034,
0.04859953746199608,
0.0537862591445446,
0.010765625163912773,
-0.03881777450442314,
-0.009665806777775288,
-0.018368421122431755,
-0.006397406104952097,
-0.006880444008857012,
-0.003718776162713766,
-0.025870051234960556,
0.010600303299725056,
0.07209265977144241
] |
PMID:25191 | Benzodiazepine receptors: labeling in intact animals with [3H] flunitrazepam. | [3H]Flumitrazepam appears to label specific benzodiazepine receptors in vitro after i.v. injection in mice. Benzodiazepine potencies in reducing [3H]flunitrazepam binding in vivo correspond to pharmacological potencies and parallel relative affinites for [3H]flunitrazepam binding sites in isolated brain membranes. However, 50% occupation of [3H]-flunitrazepam sites by benzodiazepines in vivo requires brain concentrations of the drugs about 1000 times higher than their Ki values for the binding sites in vitro. In pharmacologically active doses sodium pentobarbital, strychnine, picrotoxin and bicuculline fail to influence [3H] flunitrazepam binding in vivo. | Benzodiazepine receptors: labeling in intact animals with [3H] flunitrazepam. [3H]Flumitrazepam appears to label specific benzodiazepine receptors in vitro after i.v. injection in mice. Benzodiazepine potencies in reducing [3H]flunitrazepam binding in vivo correspond to pharmacological potencies and parallel relative affinites for [3H]flunitrazepam binding sites in isolated brain membranes. However, 50% occupation of [3H]-flunitrazepam sites by benzodiazepines in vivo requires brain concentrations of the drugs about 1000 times higher than their Ki values for the binding sites in vitro. In pharmacologically active doses sodium pentobarbital, strychnine, picrotoxin and bicuculline fail to influence [3H] flunitrazepam binding in vivo. | [
-0.010982218198478222,
-0.0066161565482616425,
-0.013439497910439968,
0.03805297240614891,
0.03330426663160324,
0.018899232149124146,
0.056491173803806305,
0.06670643389225006,
-0.02318897843360901,
-0.019883329048752785,
-0.034587468951940536,
-0.026200516149401665,
-0.0036417143419384956,
0.025012599304318428,
-0.03822518140077591,
-0.024656817317008972,
-0.02264229767024517,
-0.02136242389678955,
-0.013569784350693226,
-0.04291903227567673,
-0.024876128882169724,
-0.04213038086891174,
0.012834490276873112,
0.03782259300351143,
0.02107168361544609,
0.02358107827603817,
-0.0036212094128131866,
-0.030665544793009758,
-0.035260967910289764,
-0.18777398765087128,
-0.01887550763785839,
0.028531700372695923,
0.00929227564483881,
-0.08001284301280975,
-0.07588820904493332,
-0.005820324178785086,
-0.019095057621598244,
0.05123349279165268,
-0.007722924463450909,
-0.019740818068385124,
0.08029505610466003,
0.07709184288978577,
-0.022678786888718605,
-0.003947696182876825,
-0.0009040014119818807,
-0.009576518088579178,
0.01725127547979355,
-0.02545405738055706,
0.10602599382400513,
-0.0062424009665846825,
0.0418558269739151,
-0.05866945534944534,
0.05201611667871475,
0.04887690395116806,
-0.013218128122389317,
-0.008232214488089085,
0.000563722918741405,
-0.030704420059919357,
-0.039189692586660385,
0.03226909413933754,
-0.03048798255622387,
0.03051997348666191,
-0.14953677356243134,
0.02803223766386509,
0.05165383219718933,
-0.029178161174058914,
0.030074499547481537,
-0.026353852823376656,
0.031875934451818466,
0.11716089397668839,
-0.046465955674648285,
-0.03799119219183922,
-0.047008320689201355,
0.015220616944134235,
0.054691098630428314,
-0.056651853024959564,
0.0275515578687191,
0.005406796466559172,
0.0004487266414798796,
-0.07263258099555969,
-0.005554461386054754,
-0.005720282904803753,
-0.0012604959774762392,
-0.02631167322397232,
0.005359243601560593,
0.004139268770813942,
0.10338624566793442,
-0.04131084680557251,
0.01485894899815321,
0.04076844081282616,
-0.013266308233141899,
-0.0366135910153389,
0.000288323120912537,
-0.04494307190179825,
-0.02284170314669609,
0.056586846709251404,
0.010034586302936077,
0.05038934201002121,
-0.027321074157953262,
0.3517712652683258,
0.010683189146220684,
0.026563072577118874,
-0.08496397733688354,
0.010222046636044979,
-0.004138640593737364,
-0.0003015408292412758,
-0.019073186442255974,
0.002328974660485983,
-0.07353578507900238,
0.0677030086517334,
0.012320127338171005,
-0.010532851330935955,
0.05978682264685631,
-0.005594721995294094,
-0.018863553181290627,
-0.024663634598255157,
0.048533160239458084,
0.0020767708774656057,
0.06802071630954742,
0.03992990404367447,
-0.013302371837198734,
0.01790279522538185,
0.053671944886446,
0.04437417909502983,
0.10266981273889542,
0.02485780045390129,
-0.04875195026397705,
0.044683828949928284,
0.051847610622644424,
0.014646410010755062,
0.047499485313892365,
-0.010261445306241512,
0.017597585916519165,
0.09394760429859161,
0.004545634146779776,
-0.006736380979418755,
0.04079394415020943,
-0.038354258984327316,
0.00029170236666686833,
-0.009620596654713154,
0.026407089084386826,
-0.012104940600693226,
0.007600459270179272,
-0.06695272028446198,
-0.040311623364686966,
0.11709906160831451,
-0.036394961178302765,
-0.035734888166189194,
-0.05798497796058655,
-0.007612921763211489,
-0.0020461000967770815,
0.019262515008449554,
-0.010505050420761108,
0.08168182522058487,
-0.050613705068826675,
0.049987178295850754,
0.01164502277970314,
0.007065127603709698,
-0.04081913083791733,
0.016186727210879326,
0.0015077502466738224,
-0.058138228952884674,
0.03075973130762577,
0.03298135846853256,
-0.0305721927434206,
0.008188490755856037,
-0.023422084748744965,
-0.004016181919723749,
-0.04372630640864372,
0.03436838090419769,
-0.03317131847143173,
0.053022921085357666,
-0.03932557255029678,
-0.06322482228279114,
-0.09522996842861176,
0.036706775426864624,
-0.01118715200573206,
0.007269056048244238,
0.008839746005833149,
-0.01041813287883997,
-0.03527475520968437,
-0.030719175934791565,
-0.053841061890125275,
0.02341659553349018,
0.03582806885242462,
0.01410619355738163,
-0.026133550330996513,
-0.05135359615087509,
-0.04391874000430107,
0.026534713804721832,
-0.03777265176177025,
0.0012253785971552134,
-0.013796994462609291,
-0.044371213763952255,
-0.04001456871628761,
-0.04452274739742279,
-0.07345310598611832,
0.03435339778661728,
-0.002541779773309827,
-0.03663729131221771,
0.11541179567575455,
0.048196036368608475,
-0.01423610933125019,
0.08789418637752533,
0.028256000950932503,
0.028701625764369965,
-0.005726044066250324,
0.010294067673385143,
0.09340062737464905,
-0.027746926993131638,
0.002410857006907463,
0.05748944729566574,
0.009054020047187805,
0.004096924792975187,
-0.01726771704852581,
0.024022389203310013,
-0.021182019263505936,
0.005547730717808008,
0.03982178494334221,
0.06000092998147011,
0.029698345810174942,
-0.012364144437015057,
-0.0034842882305383682,
-0.3225962519645691,
-0.0364144966006279,
0.039215248078107834,
0.03252487629652023,
0.062435831874608994,
0.008995743468403816,
0.049873389303684235,
-0.016553835943341255,
0.006369501352310181,
-0.05714360997080803,
-0.02669198252260685,
-0.005245327949523926,
-0.06114008277654648,
-0.06971307843923569,
-0.021456656977534294,
0.0456562303006649,
0.02505968138575554,
-0.04753332585096359,
0.03771152347326279,
-0.047318264842033386,
-0.030520489439368248,
0.004584350157529116,
-0.0875672772526741,
0.053150344640016556,
0.043490830808877945,
-0.00588513258844614,
0.0709848627448082,
0.00960753858089447,
-0.04480111971497536,
-0.029270920902490616,
0.05003669485449791,
0.04699467122554779,
-0.02437111921608448,
-0.11660663783550262,
-0.0192663986235857,
0.01233058050274849,
-0.02152494713664055,
0.02075939066708088,
0.03581488877534866,
-0.017621146515011787,
-0.05748030170798302,
0.054687365889549255,
0.024315843358635902,
-0.0697004646062851,
-0.05921679735183716,
-0.011143400333821774,
0.013671253807842731,
-0.02665303274989128,
0.023535314947366714,
-0.026603326201438904,
0.011219015344977379,
0.030832713469862938,
-0.02415074221789837,
-0.07001058012247086,
-0.007697319146245718,
0.018351620063185692,
-0.059874195605516434,
0.03191542625427246,
-0.07884081453084946,
-0.004029880743473768,
-0.0005314337322488427,
0.012418309226632118,
0.0029130340553820133,
-0.0008908016025088727,
0.024790573865175247,
0.0538407601416111,
0.009805996902287006,
-0.030823776498436928,
0.033242855221033096,
0.06204119697213173,
0.013077931478619576,
0.09822391718626022,
-0.001350375241599977,
0.014563641510903835,
0.10088123381137848,
-0.010382520966231823,
-0.04458167776465416,
-0.022125571966171265,
-0.09365136176347733,
0.029347054660320282,
-0.06696692854166031,
-0.020083658397197723,
0.0579826682806015,
0.025884997099637985,
0.01911303959786892,
-0.002606830792501569,
0.009428285993635654,
-0.03258238360285759,
0.0677638128399849,
0.021630946546792984,
0.009054161608219147,
0.01970907300710678,
-0.04843718558549881,
-0.04568518325686455,
0.019616255536675453,
0.06290939450263977,
-0.24619773030281067,
0.033144544810056686,
0.042763229459524155,
0.040409695357084274,
0.046609118580818176,
0.022213250398635864,
0.05138268694281578,
-0.04206046462059021,
-0.01662903092801571,
-0.022529959678649902,
-0.021405059844255447,
-0.035597339272499084,
0.020594974979758263,
0.06558740139007568,
-0.01549022551625967,
0.05739223584532738,
0.07728073745965958,
-0.030509643256664276,
0.01724880374968052,
-0.026952745392918587,
0.03604309633374214,
0.0061909048818051815,
0.11640850454568863,
0.0007292745285667479,
0.021820206195116043,
0.04006518796086311,
-0.01204264722764492,
-0.007575973868370056,
-0.02914636954665184,
0.030776916071772575,
-0.04182137921452522,
-0.04925667494535446,
0.07702013850212097,
-0.015151556581258774,
0.025932155549526215,
0.0318404957652092,
-0.05180532485246658,
-0.04127435013651848,
-0.03320452570915222,
0.025983143597841263,
-0.010293622501194477,
0.027319397777318954,
-0.08670253306627274,
-0.02832341380417347,
0.08473522216081619,
-0.05990247055888176,
-0.044550567865371704,
-0.016443798318505287,
0.00338184367865324,
-0.02308395691215992,
-0.051139187067747116,
-0.012855109758675098,
-0.042562130838632584,
0.045872241258621216,
0.06673109531402588,
-0.025190988555550575,
0.04017733782529831,
-0.0435279980301857,
-0.026074819266796112,
0.0353471077978611,
-0.026375222951173782,
-0.016571929678320885,
-0.030122794210910797,
0.03652726486325264,
0.014761926606297493
] |
PMID:25194 | Amnesic effects of intravenous diazepam and lorazepam. | The amnesic effects of 2 benzodiazepine drugs, diazepam (Valium) and lorazepam (Ativan), have been investigated. Some of the effects were similar to those of certain clinical amnesic syndromes. The effects were more extensive than previous work has indicated. | Amnesic effects of intravenous diazepam and lorazepam. The amnesic effects of 2 benzodiazepine drugs, diazepam (Valium) and lorazepam (Ativan), have been investigated. Some of the effects were similar to those of certain clinical amnesic syndromes. The effects were more extensive than previous work has indicated. | [
-0.024602854624390602,
-0.013368687592446804,
0.03632570058107376,
0.06240997835993767,
0.007861094549298286,
-0.034181900322437286,
0.042671963572502136,
0.04072628170251846,
-0.027480097487568855,
-0.08583346009254456,
-0.03707778826355934,
0.03620532155036926,
-0.0011044725542888045,
0.02867138758301735,
0.02988489903509617,
-0.026021236553788185,
0.004407738335430622,
-0.04075207933783531,
-0.04786727577447891,
0.020840412005782127,
-0.05094924196600914,
0.04277420789003372,
0.0070955343544483185,
-0.010700957849621773,
0.01670956239104271,
-0.020453691482543945,
0.019659649580717087,
0.03842904046177864,
-0.022874945774674416,
-0.14986127614974976,
0.016777295619249344,
0.04015028104186058,
0.01488547958433628,
-0.07227560132741928,
-0.037758614867925644,
-0.0330469012260437,
-0.027348393574357033,
0.0821942687034607,
-0.022022990509867668,
-0.009718742221593857,
0.10161703079938889,
0.04720994457602501,
-0.02144293300807476,
-0.05102422460913658,
-0.002444180892780423,
-0.03004113957285881,
0.013549991883337498,
-0.020824037492275238,
0.0990120992064476,
-0.0669667199254036,
0.003177003702148795,
-0.01665288396179676,
0.08954396843910217,
0.04877223074436188,
0.009199102409183979,
-0.028069229796528816,
-0.02663266845047474,
0.015575365163385868,
-0.053685788065195084,
0.061821140348911285,
0.012533931992948055,
0.006584370508790016,
-0.08450647443532944,
0.01185319572687149,
-0.052890852093696594,
0.027257436886429787,
-0.00627322681248188,
-0.046934694051742554,
0.01872168481349945,
0.04301225021481514,
-0.06092384085059166,
-0.016404084861278534,
0.013086827471852303,
0.03274618089199066,
0.034233082085847855,
-0.015963608399033546,
0.03243497014045715,
-0.026030102744698524,
-0.0680655837059021,
-0.032488785684108734,
0.04940130189061165,
-0.07024766504764557,
0.012043868191540241,
-0.07342708855867386,
0.03339258208870888,
-0.010355447418987751,
0.03492935001850128,
-0.005277994088828564,
0.051715999841690063,
-0.008769260719418526,
0.0544327050447464,
-0.004951098468154669,
0.014532586559653282,
-0.040134917944669724,
0.04539843276143074,
0.046321794390678406,
-0.002998211421072483,
-0.04038051515817642,
-0.0006623844965361059,
0.3496381640434265,
0.02170773409307003,
0.025074481964111328,
-0.03229079395532608,
-0.014109226875007153,
0.01926371082663536,
-0.0170709528028965,
0.016128038987517357,
-0.00637705996632576,
0.01742013916373253,
0.011722393333911896,
0.032906707376241684,
-0.025781283155083656,
0.09186434000730515,
0.005977540742605925,
-0.07615362107753754,
0.019111672416329384,
0.11051245778799057,
0.015684770420193672,
-0.0006088676163926721,
0.024414952844381332,
0.0030882530845701694,
-0.005673027131706476,
-0.04296782240271568,
-0.017178865149617195,
0.07145818322896957,
-0.02277403138577938,
-0.05013475939631462,
0.10811313986778259,
-0.019386161118745804,
-0.0010161843383684754,
0.010432989336550236,
-0.009901566430926323,
0.0796954408288002,
0.010042037814855576,
0.029297063127160072,
0.040215179324150085,
-0.024703944101929665,
-0.03189162537455559,
0.05534694716334343,
0.018013080582022667,
0.020009897649288177,
-0.0358484648168087,
-0.02256658673286438,
-0.0931830108165741,
-0.05087093263864517,
0.1000143364071846,
-0.032869432121515274,
-0.016970373690128326,
-0.02452060393989086,
0.06277250498533249,
-0.0625656470656395,
0.0020052301697432995,
-0.03888171166181564,
0.054223448038101196,
-0.015011058188974857,
0.006246854551136494,
0.02343754656612873,
0.03700929507613182,
-0.045065443962812424,
0.015768811106681824,
-0.024876639246940613,
-0.012193601578474045,
0.017210036516189575,
0.08585688471794128,
-0.020783325657248497,
0.0415465421974659,
-0.010651973076164722,
-0.024440476670861244,
-0.07112156599760056,
0.014248906634747982,
-0.006334973964840174,
0.027030110359191895,
-0.05617111921310425,
-0.02306330017745495,
0.029603902250528336,
0.01770358718931675,
0.023662595078349113,
-0.02305535599589348,
-0.032980818301439285,
-0.005513662472367287,
0.03668233007192612,
0.03014916181564331,
0.013825720176100731,
0.018024543300271034,
-0.00926655251532793,
-0.01886948198080063,
-0.023034632205963135,
0.01479492336511612,
0.03838953748345375,
0.029712948948144913,
-0.023883454501628876,
-0.003419369226321578,
-0.0825587585568428,
-0.026075726374983788,
-0.061970166862010956,
-0.035099249333143234,
-0.09038963913917542,
-0.0034540481865406036,
0.007820111699402332,
-0.04942495375871658,
0.07720623165369034,
0.07533860206604004,
0.04827398061752319,
0.044967494904994965,
0.014716009609401226,
0.08159446716308594,
-0.023168493062257767,
-0.00807675626128912,
0.0055964612402021885,
-0.012960664927959442,
-0.01559008751064539,
0.0073209465481340885,
0.04293906316161156,
0.031673919409513474,
-0.041360676288604736,
0.01842558942735195,
-0.08681881427764893,
0.03814364969730377,
0.03477603569626808,
0.05283132568001747,
0.06300216168165207,
-0.036712318658828735,
0.028268873691558838,
-0.303069531917572,
-0.04271800071001053,
0.0636586993932724,
-0.016761107370257378,
0.0723118782043457,
-0.055903587490320206,
0.010814015753567219,
-0.03398599475622177,
-0.06389325112104416,
-0.05298373103141785,
-0.0857682153582573,
0.024358252063393593,
-0.04047206789255142,
-0.003917234018445015,
0.025710703805088997,
-0.02270647883415222,
0.006365950684994459,
-0.044899631291627884,
0.030253339558839798,
-0.053195852786302567,
-0.05361077934503555,
-0.020015785470604897,
-0.03219699487090111,
0.0084617855027318,
0.022396903485059738,
-0.03530687466263771,
0.06838154047727585,
0.024114029482007027,
-0.03879186138510704,
-0.0633508488535881,
0.0017279009334743023,
0.07997274398803711,
0.0505821667611599,
-0.05519074574112892,
0.06549951434135437,
0.025002971291542053,
0.008142340928316116,
-0.01427406445145607,
0.004502313211560249,
-0.0037512362468987703,
-0.12167181819677353,
-0.03215714544057846,
0.028393354266881943,
0.015695635229349136,
-0.02565794251859188,
0.02974475733935833,
-0.012246803380548954,
-0.05832253396511078,
-0.0426567941904068,
-0.00009599067561794072,
-0.008892533369362354,
0.008951481431722641,
-0.021808743476867676,
-0.029170341789722443,
0.0694214254617691,
-0.022924428805708885,
-0.1196490153670311,
0.009579473175108433,
0.006405477877706289,
-0.01858878694474697,
0.004459363874047995,
0.036444246768951416,
0.07883141189813614,
-0.07182871550321579,
0.0010830448009073734,
-0.05066275969147682,
0.003066195407882333,
0.017903588712215424,
0.06599865108728409,
0.030296524986624718,
-0.03222194314002991,
0.1668364256620407,
-0.020392153412103653,
-0.06918186694383621,
0.07522527873516083,
0.04072839394211769,
0.0013517801417037845,
-0.022824278101325035,
-0.05823521316051483,
0.026136258617043495,
-0.08965036273002625,
-0.018077146261930466,
0.03007025644183159,
0.02396244928240776,
-0.043018657714128494,
-0.002631633309647441,
-0.03874408081173897,
-0.023963777348399162,
0.01330992579460144,
0.00013780518202111125,
0.010961869731545448,
0.016675425693392754,
0.002607543719932437,
-0.0044405520893633366,
-0.03862037509679794,
0.020912056788802147,
-0.22277392446994781,
0.006794656626880169,
-0.02817898616194725,
0.05239357799291611,
0.04995649307966232,
0.04413412883877754,
-0.02151280641555786,
-0.007797646336257458,
-0.008852998726069927,
-0.036171671003103256,
-0.005847345106303692,
-0.0250451248139143,
0.035769376903772354,
0.009820403531193733,
-0.03985109180212021,
0.027095425873994827,
0.08386299014091492,
0.02584303729236126,
0.005275915376842022,
-0.018450861796736717,
0.020993361249566078,
0.083746999502182,
0.13506223261356354,
0.02217257022857666,
0.058347832411527634,
0.06382566690444946,
0.06170235574245453,
0.041999585926532745,
-0.0328514464199543,
0.0885818600654602,
0.004101425874978304,
0.0013898388715460896,
0.042032308876514435,
0.016110440716147423,
0.011934134177863598,
-0.010975237935781479,
-0.12877793610095978,
0.020522113889455795,
0.027865618467330933,
0.04509177803993225,
-0.005475013982504606,
0.04227052628993988,
-0.06612425297498703,
-0.032595545053482056,
0.07630739361047745,
-0.07290323823690414,
0.0036483106669038534,
-0.0421271026134491,
-0.022357560694217682,
0.001957931322976947,
-0.05161409080028534,
-0.024835264310240746,
-0.009448637254536152,
0.052835531532764435,
0.05277840420603752,
-0.014154722914099693,
-0.02693016640841961,
-0.03149496391415596,
0.0632849782705307,
0.03102288395166397,
-0.048178113996982574,
-0.04810461401939392,
-0.009614160284399986,
0.007605849765241146,
-0.021886562928557396
] |
PMID:25195 | Neutral proteases in the guinea-pig lymphocytes. | Partial purification of neutral proteolytic enzymes in guinea-pig lymphocytes yielded 2 enzymes. Both enzymes were heat-labile and inhibited by thiol reagents. The molecular weights were more than 200,000 and 150,000-200,000, and optimal pH around 9 and 8, respectively. | Neutral proteases in the guinea-pig lymphocytes. Partial purification of neutral proteolytic enzymes in guinea-pig lymphocytes yielded 2 enzymes. Both enzymes were heat-labile and inhibited by thiol reagents. The molecular weights were more than 200,000 and 150,000-200,000, and optimal pH around 9 and 8, respectively. | [
-0.11549201607704163,
-0.006190687883645296,
-0.07946646958589554,
0.0019130761502310634,
-0.006549567449837923,
-0.03566449508070946,
0.07168633490800858,
0.061233922839164734,
-0.00546122994273901,
0.012639877386391163,
0.004885616712272167,
-0.08111637830734253,
-0.028234999626874924,
0.042911410331726074,
-0.008448243141174316,
-0.01792047545313835,
0.020216483622789383,
0.011163904331624508,
-0.1035928875207901,
-0.010177478194236755,
-0.04955113306641579,
0.017076613381505013,
0.03276599943637848,
-0.03588328883051872,
0.06046012416481972,
-0.02271912805736065,
-0.032572753727436066,
0.028820356354117393,
-0.019016558304429054,
-0.18305471539497375,
0.035642120987176895,
-0.032283201813697815,
-0.03377144783735275,
-0.014461823739111423,
0.01725362055003643,
0.02232895977795124,
0.007982894778251648,
-0.05544910207390785,
-0.018038121983408928,
0.03892718628048897,
0.0566289946436882,
-0.00788606982678175,
-0.012143122963607311,
0.00847591832280159,
0.07091592997312546,
-0.006747394800186157,
-0.07864519953727722,
-0.02079731971025467,
0.024917447939515114,
-0.012277656234800816,
0.01768382266163826,
0.04657411947846413,
-0.04746866971254349,
0.02357489801943302,
-0.010912406258285046,
-0.002457099035382271,
0.057231586426496506,
0.07904084026813507,
-0.0467408187687397,
-0.010557478293776512,
-0.011082774959504604,
-0.020756248384714127,
-0.13535158336162567,
0.062061768025159836,
0.062174875289201736,
-0.023519836366176605,
0.0000072801285568857566,
-0.07556744664907455,
-0.042406823486089706,
0.04158090054988861,
-0.025693679228425026,
0.03646761178970337,
0.05945669114589691,
-0.03623286262154579,
0.02265898324549198,
0.04341168701648712,
-0.007846572436392307,
0.07151191681623459,
0.07778360694646835,
0.001547925523482263,
-0.02303175814449787,
-0.0030514101963490248,
0.055038485676050186,
0.017256848514080048,
-0.017902346327900887,
0.015576173551380634,
-0.0328410305082798,
-0.008814793080091476,
0.05417291074991226,
-0.030064987018704414,
-0.0266211349517107,
-0.04584769532084465,
0.06189098581671715,
-0.01629577949643135,
-0.015453001484274864,
0.02464323863387108,
0.07095254957675934,
-0.02037978358566761,
0.04145289584994316,
0.3743472099304199,
-0.016855766996741295,
-0.002588574541732669,
-0.07973945140838623,
-0.009227641858160496,
-0.00011924357386305928,
-0.04311691224575043,
-0.025078019127249718,
-0.0033298395574092865,
-0.05236795172095299,
0.09027395397424698,
0.06921234726905823,
0.047800250351428986,
0.00037561150384135544,
-0.06885020434856415,
0.013035822659730911,
-0.0008168455096893013,
0.013364532962441444,
-0.006554007064551115,
-0.015809588134288788,
0.037937793880701065,
-0.02062561921775341,
-0.03316239267587662,
0.036911800503730774,
-0.03441419452428818,
0.04178347438573837,
0.022456852719187737,
-0.007666671648621559,
0.08505870401859283,
0.02358025126159191,
-0.003079126589000225,
0.028368769213557243,
0.05338800698518753,
-0.026240546256303787,
0.025165289640426636,
-0.005325483623892069,
-0.03719133883714676,
0.019789928570389748,
-0.024388886988162994,
0.033591389656066895,
-0.03495004400610924,
-0.004776677582412958,
-0.04370007663965225,
-0.06003839522600174,
-0.09125365316867828,
0.005192649085074663,
0.06957481056451797,
-0.04337223991751671,
0.053299710154533386,
-0.042486634105443954,
-0.01844709925353527,
0.017175689339637756,
0.0743914470076561,
-0.006826591677963734,
0.06250249594449997,
0.016235262155532837,
0.03817429021000862,
-0.010652345605194569,
0.02870939113199711,
0.05523256957530975,
-0.0025436931755393744,
-0.06694365292787552,
-0.028157193213701248,
-0.026834825053811073,
0.022523462772369385,
0.044672735035419464,
0.02142133191227913,
-0.017770199105143547,
0.0013443822972476482,
0.012479736469686031,
-0.010126767680048943,
0.005699747242033482,
-0.00818816851824522,
-0.04707812890410423,
0.00844965036958456,
-0.06889662146568298,
0.009100040420889854,
-0.04014991596341133,
0.053276073187589645,
0.03953535854816437,
0.038475196808576584,
-0.07013401389122009,
-0.049601566046476364,
-0.017587486654520035,
0.005388960242271423,
0.05608677864074707,
-0.01363924890756607,
-0.04461830109357834,
-0.011407141573727131,
0.030418535694479942,
0.08836495876312256,
-0.04027114808559418,
-0.0466599203646183,
-0.11515127867460251,
-0.02957886829972267,
-0.05388640612363815,
-0.021991634741425514,
-0.014931934885680676,
-0.02201288379728794,
-0.003999408334493637,
-0.022991925477981567,
0.08363644033670425,
0.023341625928878784,
0.027066785842180252,
0.03505326807498932,
-0.011320007033646107,
0.07142354547977448,
0.03900158777832985,
0.0076386211439967155,
0.09757869690656662,
-0.0011594969546422362,
-0.0603896863758564,
-0.013842636719346046,
-0.02961919829249382,
-0.03289851173758507,
-0.01978721283376217,
0.06073169782757759,
0.008168506436049938,
-0.013674259185791016,
0.09174499660730362,
0.0001235227391589433,
-0.055695340037345886,
-0.049526557326316833,
-0.08709678798913956,
-0.2624320387840271,
-0.013941584154963493,
0.01866261288523674,
-0.012180446647107601,
0.04944120720028877,
0.001928807469084859,
0.004305623471736908,
-0.037324871867895126,
-0.0011325340019538999,
-0.03817843645811081,
0.09272749722003937,
0.06059415638446808,
-0.07065419852733612,
-0.029230132699012756,
0.050539858639240265,
0.07580606639385223,
-0.028177890926599503,
-0.005685421172529459,
-0.0008608780917711556,
0.06098509952425957,
0.01690642349421978,
-0.029539069160819054,
0.02532203309237957,
0.029112594202160835,
0.028003349900245667,
0.07975298911333084,
0.14180395007133484,
0.0026005578693002462,
0.031051667407155037,
0.026128116995096207,
0.030121007934212685,
0.003503520740196109,
-0.0061665549874305725,
-0.054816003888845444,
-0.06011558324098587,
-0.016147978603839874,
-0.05258642137050629,
-0.007964164018630981,
0.023942414671182632,
0.024972576647996902,
-0.002524019218981266,
0.006119637284427881,
-0.03461442515254021,
-0.09407560527324677,
-0.012540883384644985,
-0.02010379359126091,
0.0546649806201458,
0.0051349857822060585,
0.0021899573039263487,
0.008748693391680717,
0.004479332361370325,
-0.008510054089128971,
-0.005076407454907894,
-0.0032974977511912584,
-0.0030688794795423746,
0.0160213615745306,
-0.040656451135873795,
0.036600496619939804,
-0.03055601194500923,
0.019675113260746002,
0.020671624690294266,
-0.04711982607841492,
-0.03881185129284859,
0.059525877237319946,
-0.056692011654376984,
-0.01392465177923441,
0.061959486454725266,
-0.02682674489915371,
0.01988384500145912,
-0.004691518843173981,
0.017963139340281487,
0.09247294068336487,
0.025740772485733032,
-0.008090583607554436,
-0.019247492775321007,
-0.011343834921717644,
0.03907564654946327,
-0.018115058541297913,
-0.05309031158685684,
0.03262615203857422,
0.03923751041293144,
-0.05162453278899193,
0.027057692408561707,
0.016344398260116577,
0.014395024627447128,
0.02276950515806675,
0.05763934552669525,
-0.029404429718852043,
0.009899292141199112,
0.013005461543798447,
0.010603572241961956,
-0.0017067455919459462,
-0.017503809183835983,
-0.050841547548770905,
-0.014259565621614456,
-0.0059898290783166885,
-0.2393818497657776,
-0.027648622170090675,
0.029902512207627296,
-0.053880929946899414,
-0.015487512573599815,
0.00985728483647108,
0.04527141898870468,
-0.0011943317949771881,
-0.030043918639421463,
0.032417092472314835,
0.06123354658484459,
-0.0007788127986714244,
0.06566528975963593,
0.0019524305826053023,
-0.0505693256855011,
0.028583412989974022,
0.047260913997888565,
-0.028895743191242218,
-0.015943683683872223,
-0.010995439253747463,
-0.021368389949202538,
-0.12460552155971527,
0.14667567610740662,
0.02166789211332798,
0.04064568877220154,
0.05549526587128639,
-0.00016755328397266567,
0.007411896716803312,
-0.06720174849033356,
0.045439187437295914,
0.015520161017775536,
0.010483751073479652,
0.05845797434449196,
-0.027781836688518524,
-0.002143952064216137,
0.06729283928871155,
-0.023957043886184692,
-0.0035930313169956207,
-0.01847381331026554,
0.032038453966379166,
-0.05038941279053688,
0.01723669283092022,
-0.08100325614213943,
-0.0652131661772728,
0.05822894722223282,
-0.11470159888267517,
0.000467452424345538,
-0.03557994216680527,
-0.004723373334854841,
-0.004267425276339054,
-0.017395444214344025,
-0.06224576756358147,
0.039774082601070404,
0.009940683841705322,
0.04233042523264885,
-0.051786717027425766,
0.026579435914754868,
-0.025409625843167305,
-0.012520904652774334,
0.02263220027089119,
0.02917753905057907,
0.011212374083697796,
-0.005223424639552832,
0.12074874341487885,
-0.021994665265083313
] |
PMID:25197 | Effect of age on T cell differentiation. | A brief overview of the area of T cell aging is presented by first discussing the age-related changes in T cell activities, and then by focusing attention on the possible mechanisms that may be responsible for the decline. Present evidence indicates that thymic involution precedes and therefore may be responsible for the age-dependent decline in the ability of the immune system to generate functional T cells. At this time, it appears that the primary effect of thymic involution is on a T cell differentiation pathway affecting the more mature T cells first with time, and then the less mature T cells. Thus, the thymus may be the aging clock for the immune system. Further studies should be centered around processes regulating growth and atrophy of the thymus. | Effect of age on T cell differentiation. A brief overview of the area of T cell aging is presented by first discussing the age-related changes in T cell activities, and then by focusing attention on the possible mechanisms that may be responsible for the decline. Present evidence indicates that thymic involution precedes and therefore may be responsible for the age-dependent decline in the ability of the immune system to generate functional T cells. At this time, it appears that the primary effect of thymic involution is on a T cell differentiation pathway affecting the more mature T cells first with time, and then the less mature T cells. Thus, the thymus may be the aging clock for the immune system. Further studies should be centered around processes regulating growth and atrophy of the thymus. | [
-0.030403627082705498,
0.0025985604152083397,
0.009525923989713192,
0.04342581331729889,
-0.0315106026828289,
0.008288021199405193,
0.05042530596256256,
0.06508810073137283,
-0.05021454393863678,
-0.030587496235966682,
0.03976941481232643,
-0.043700046837329865,
0.015690181404352188,
0.02139892242848873,
-0.05089879408478737,
0.030626514926552773,
0.0036464908625930548,
-0.003995375707745552,
-0.09902816265821457,
-0.008858979679644108,
-0.043852321803569794,
-0.03598756343126297,
0.018081439658999443,
-0.06200314685702324,
0.014943930320441723,
-0.03989892452955246,
0.0210660919547081,
-0.006693946663290262,
-0.024558261036872864,
-0.1583486944437027,
0.07265318930149078,
-0.03647482022643089,
-0.06208387762308121,
0.00934684555977583,
-0.050723008811473846,
0.03844945505261421,
-0.022617770358920097,
-0.04597157984972,
-0.006999654229730368,
0.022823521867394447,
0.005889867898076773,
0.023006869480013847,
-0.01835314743220806,
-0.019746748730540276,
-0.05097007751464844,
-0.0022099062334746122,
-0.08442532271146774,
-0.017616473138332367,
0.00184480135794729,
0.03686734661459923,
-0.03739170357584953,
-0.013396921567618847,
0.01855349913239479,
0.12435977160930634,
-0.04851129278540611,
0.016942834481596947,
-0.009458685293793678,
0.09891746938228607,
0.04124840348958969,
0.018895450979471207,
0.017406148836016655,
-0.020170601084828377,
-0.13312843441963196,
0.07223641872406006,
0.12002041935920715,
0.006108579691499472,
0.046678390353918076,
0.04807601496577263,
0.041958779096603394,
-0.014722908847033978,
-0.07000648230314255,
0.0525643415749073,
0.02258281037211418,
0.06868093460798264,
0.04272777959704399,
-0.007169135846197605,
-0.07607411593198776,
-0.051298514008522034,
0.04596647992730141,
0.01622098870575428,
0.09927539527416229,
0.029972052201628685,
-0.005938575603067875,
-0.01471442636102438,
-0.024428214877843857,
-0.05774867162108421,
-0.006353525910526514,
-0.09719222038984299,
-0.019661473110318184,
-0.004998557735234499,
0.0054489909671247005,
-0.022813307121396065,
-0.03496816009283066,
0.03429562598466873,
-0.03971528261899948,
-0.0007339945295825601,
0.02497890777885914,
-0.038141027092933655,
0.008600876666605473,
0.3678167164325714,
-0.027398990467190742,
0.029914850369095802,
0.014147821813821793,
0.04248218238353729,
-0.012430495582520962,
-0.06446654349565506,
0.0364772193133831,
0.015772759914398193,
-0.021586593240499496,
0.08659461885690689,
-0.011516054160892963,
-0.006664050742983818,
-0.05193185433745384,
0.028830189257860184,
0.03487859293818474,
0.013294500298798084,
0.07451070845127106,
0.02302105724811554,
0.00823304709047079,
0.061125244945287704,
0.039809711277484894,
-0.04089707136154175,
0.030402356758713722,
-0.01337120495736599,
0.04415065422654152,
0.050231002271175385,
-0.013771655969321728,
0.14095450937747955,
0.04663636535406113,
-0.030083654448390007,
0.08492463082075119,
-0.0318918451666832,
-0.04208049178123474,
0.06564652919769287,
0.03195282444357872,
-0.021978305652737617,
-0.03773689270019531,
0.003017114708200097,
0.024697812274098396,
-0.042846810072660446,
-0.035139694809913635,
-0.061046794056892395,
-0.024315640330314636,
-0.09195934981107712,
0.0011899967212229967,
0.10825438052415848,
0.060682132840156555,
0.02665768377482891,
0.0028021754696965218,
0.007903854362666607,
-0.010599433444440365,
0.05209134519100189,
0.033438317477703094,
0.04378630965948105,
-0.002465739380568266,
0.030053365975618362,
-0.0837307721376419,
0.011898359283804893,
0.010771166533231735,
-0.04947979003190994,
-0.03100995533168316,
-0.005599010270088911,
-0.06132319197058678,
0.0887259691953659,
0.027941333130002022,
0.015110575594007969,
-0.01421188935637474,
-0.01857226900756359,
0.055721085518598557,
0.0020132395438849926,
0.002372348215430975,
0.022274646908044815,
-0.06722885370254517,
-0.025226976722478867,
0.016145776957273483,
0.023582234978675842,
-0.040407292544841766,
-0.00933795515447855,
-0.013963731937110424,
-0.004884915892034769,
0.03474263474345207,
-0.0018008145270869136,
0.015741296112537384,
-0.011400667950510979,
0.034483231604099274,
-0.038066111505031586,
0.02063114196062088,
0.02054622955620289,
0.038137927651405334,
0.027400219812989235,
-0.0041997721418738365,
0.003974510356783867,
-0.07506637275218964,
-0.05509001016616821,
-0.03265814855694771,
-0.027384107932448387,
-0.02765405736863613,
-0.01005792897194624,
-0.01015115063637495,
-0.03829243779182434,
-0.005271814297884703,
-0.021379394456744194,
-0.008578401990234852,
0.011862678453326225,
-0.018318206071853638,
0.009901785291731358,
0.03511573001742363,
-0.057271797209978104,
0.08990267664194107,
-0.043998897075653076,
-0.04228344187140465,
-0.055453937500715256,
0.021149281412363052,
0.01474457886070013,
0.031732991337776184,
0.03210759162902832,
-0.01075324323028326,
-0.06302106380462646,
0.01775365136563778,
0.020054101943969727,
0.04891088977456093,
0.0011414388427510858,
-0.03176724165678024,
-0.27254101634025574,
-0.05037258192896843,
-0.024737967178225517,
-0.03539988771080971,
-0.0021142263431102037,
-0.060742322355508804,
-0.07900184392929077,
-0.009852403774857521,
0.026042992249131203,
-0.032978612929582596,
0.07562310993671417,
0.12926296889781952,
0.01136647630482912,
-0.11134882271289825,
-0.030613768845796585,
0.03435148671269417,
0.03421627730131149,
0.02216777205467224,
0.04548686742782593,
0.012476957403123379,
0.045186515897512436,
0.025492938235402107,
0.047806885093450546,
-0.028900623321533203,
0.0397285521030426,
0.022530829533934593,
0.10113329440355301,
-0.00911425705999136,
-0.009739433415234089,
0.03789982199668884,
0.08419173210859299,
-0.033529601991176605,
-0.04386850446462631,
-0.038812823593616486,
0.04073066636919975,
-0.005750935524702072,
-0.03983980417251587,
0.020079968497157097,
0.019284656271338463,
-0.030249489471316338,
-0.044250208884477615,
-0.0116227176040411,
0.0003660494403447956,
-0.019573234021663666,
-0.04496338590979576,
0.028342466801404953,
0.029411565512418747,
0.08136898279190063,
-0.01064573135226965,
-0.06452839821577072,
0.029413023963570595,
-0.021591119468212128,
0.025657575577497482,
0.04513203352689743,
0.04452647268772125,
-0.002933412091806531,
-0.06961226463317871,
0.06990507990121841,
-0.05822311341762543,
-0.04632958769798279,
-0.0685732513666153,
0.001696026767604053,
0.012139326892793179,
-0.001089325756765902,
-0.05352066084742546,
-0.005353684537112713,
-0.018517591059207916,
0.00723491795361042,
-0.004659547936171293,
-0.025109881535172462,
0.0048454334028065205,
0.12197268754243851,
-0.059234846383333206,
0.021161111071705818,
-0.033128488808870316,
-0.0145435631275177,
-0.06543929874897003,
0.01319027878344059,
-0.04760367050766945,
-0.0035870259162038565,
-0.0009677598136477172,
-0.08638021349906921,
0.06418713182210922,
0.015554636716842651,
0.015608075074851513,
0.003159238025546074,
-0.03650091215968132,
0.05175722390413284,
-0.007879409939050674,
0.006926148198544979,
-0.04373186081647873,
0.015697995200753212,
-0.02212720364332199,
-0.03234488517045975,
-0.003943732473999262,
-0.0015889710048213601,
-0.23095521330833435,
0.05492892861366272,
0.0487649068236351,
-0.009642398916184902,
-0.023005599156022072,
-0.03275752067565918,
0.01504146121442318,
0.008016481064260006,
-0.0466395728290081,
0.024318300187587738,
0.026974741369485855,
0.01682654768228531,
0.0888444185256958,
0.016721708700060844,
-0.023408880457282066,
-0.017676323652267456,
0.03814215213060379,
-0.05427916720509529,
-0.015820037573575974,
-0.049103617668151855,
0.06553763896226883,
0.016738682985305786,
0.14281851053237915,
-0.009235727600753307,
0.033516377210617065,
0.000879005528986454,
0.005982581991702318,
0.022256813943386078,
-0.0018557278672233224,
0.0012306810822337866,
0.005554103292524815,
-0.012674648314714432,
0.07253335416316986,
0.03730509430170059,
-0.009883210062980652,
0.019565720111131668,
-0.06574132293462753,
0.010993055067956448,
-0.06542565673589706,
0.03302530199289322,
-0.06911318749189377,
0.02632158063352108,
-0.034122489392757416,
-0.03993866592645645,
0.10800640285015106,
-0.0231749527156353,
0.028451822698116302,
-0.036379337310791016,
-0.02165655419230461,
0.05374504625797272,
-0.06858864426612854,
-0.012333548627793789,
0.03389040380716324,
-0.02072574757039547,
-0.02768544852733612,
-0.026207802817225456,
0.01476925890892744,
-0.021301547065377235,
0.017861943691968918,
0.009064111858606339,
0.011002556420862675,
0.01584559679031372,
-0.02709355019032955,
0.01029526349157095,
0.05384740233421326
] |
Subsets and Splits
No community queries yet
The top public SQL queries from the community will appear here once available.