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AMX releases ‘Folk Magic 2 Boliyan‘ from his forthcoming album ‘Folk Magic 2’ and it is available as a free download. The track features the vocals of Aalam Jasdeep while lyrics are penned by Jagga Nikkuwalia. Folk Magic 2 will be releasing soon on Hi-Tech Music (UK).
AMX is a self-taught music producer/ programmer and engineer from Birmingham UK who has been working on music since 1998 with his first project being a white label underground album. Since then AMX has worked on various uncredited projects alongside doing credited work for labels such as Universal Music (India), Hi-Tech Music, DMC, Envy/Roma2, The Diamond Label, Big Drama Entertainment and Mega Rhythm. | <urn:uuid:70730d41-85ba-47fa-9b7b-66853164a8ac> | CC-MAIN-2016-44 | http://www.chakdey.com/amx-ft-aalam-jasdeep-folk-magic-2-boliyan-free-download/ | 2016-10-28T21:36:57Z | s3://commoncrawl/crawl-data/CC-MAIN-2016-44/segments/1476988725475.41/warc/CC-MAIN-20161020183845-00112-ip-10-171-6-4.ec2.internal.warc.gz | en | 0.963154 | 159 | [
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Bhairavi Goswami HD wallpapers that are high-definition and ready to download. Simply click on a preview Bhairavi Goswami Wallpaper below to view and download the full-size wallpaper.If it’s not too much trouble check our widescreen hd wallpapers underneath and bring beauty to your desktop.
Bhairavi Goswami is known well for her young acting skills. If you fan of the Bhairavi Goswami, make sure you have Bhairavi Goswami wallpaper in your collection. We bring you best of best Bhairavi Goswami high resolution wallpapers from her different films, Photoshoot,stills.Make sure you demonstrate your affection for this stunning actress by putting up her wallpaper on your PC or portable PC screen.
We think, You Enjoy the collection of Bhairavi Goswami wallpapers. beauty of the Bhairavi Goswami Wallpaper in this collection is enough to at least put a smile on your face. | <urn:uuid:2dd20d1d-db4b-48da-9f58-405c67b335f2> | CC-MAIN-2016-44 | https://www.hdwallpapersinx.com/bhairavi-goswami-hd-wallpapers/ | 2016-10-28T21:30:53Z | s3://commoncrawl/crawl-data/CC-MAIN-2016-44/segments/1476988725475.41/warc/CC-MAIN-20161020183845-00112-ip-10-171-6-4.ec2.internal.warc.gz | en | 0.941764 | 207 | [
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If you're looking for a cozy German-style bar and restaurant, look no further than the Peoria Hofbrau, 2210 NE Jefferson Ave, Peoria. I should know, my family and I practically live there, and it's where StfRon and I met one fateful Thursday more than 5 1/2 years ago.
This is the kind of place where everybody knows your name after you spend a couple of evenings there. And they do a great lunch, too, although I rarely get out of work for lunch. For a German restaurant, it's funny how people rave about the chili dogs served at lunchtime. When I do go, I go for the braunschweiger sandwich.
The Hofbrau has a great selection of beer, and if you try 80 different beers and get your tour card stamped, you can immortalize yourself on the wall of fame, or shame, depending on how you look at it. Plus, when you're halfway done with your tour, you get a free dinner.
There are lots of great dinner choices here. If you happen to be in on a Friday or Saturday night, be sure to check out the specials. They're usually fabulous. I must say, though, I have never tried any of the rabbit specials, which I'm sure are great, but I just can't bring myself to eat a rabbit.
They serve up a unique plate of nachos here, tortilla chips covered with ground beef and shredded cheddar, toasted nicely in the oven and served with sides of sour cream, salsa and hot peppers. Not exactly German fare, but delish nonetheless.
The Hofbrau has some great wine choices, too, and some of the best group of workers you could hope to find.
We've met a lot of great people over the years at the Hofbrau. One of them has recently passed away, Randy Franks. Randy was the quintessential gentleman, unless he was telling one of his jokes. He waited tables and bartended at the Hofbrau a few years ago. He was always at the ready with a light for your cigarette, another drink as the last drops were hitting your tongue, a great suggestion for an after dinner cocktail, and a good laugh. And man, did that guy have ears! He could hear a comment from clear across the bar while he was carrying on a conversation with some other people, and next thing you know, after he finished talking with them he'd be down commenting about what you had said. He could hear an emptying glass from a room away.
We've missed him over the years. Prost, Randy. | <urn:uuid:e76730c5-8af6-4581-aaeb-b89c1147eab8> | CC-MAIN-2017-47 | http://peoriapeepers.blogspot.com/2006/06/peoria-hofbrau.html | 2017-11-19T06:57:02Z | s3://commoncrawl/crawl-data/CC-MAIN-2017-47/segments/1510934805417.47/warc/CC-MAIN-20171119061756-20171119081756-00678.warc.gz | en | 0.975365 | 540 | [
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My name is Stacy Breeden and I am the Owner of Gifts of Touch Massage and Wellness Center located at 1400 Hollywood Road Suite B
Knoxville, TN 37909 (865.679.0902).
My goal is to provide therapeutic massage and reflexology modalities to the public to benefit them with relief from stress and tension, aid with health conditions, and provide regular maintenance of the body in order to achieve and maintain wellness plus a healthy body & lifestyle.
Bodywork Modalities offered:
Swedish Relaxation Massage
Reflexology *(Reflex points of the feet and/or hands)
Healing Touch Spiritual Ministry*(energy work on & off the body with prayer)
Hot Stone Massage
Deep Tissue massage
Seated chair massage | <urn:uuid:a2bf0ce6-c441-4cfc-a55d-cdb0ce1ae7a3> | CC-MAIN-2017-47 | http://giftsoftouch.massagetherapy.com/ | 2017-11-20T02:19:20Z | s3://commoncrawl/crawl-data/CC-MAIN-2017-47/segments/1510934805894.15/warc/CC-MAIN-20171120013853-20171120033853-00678.warc.gz | en | 0.842738 | 162 | [
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Passports are required. We strongly recommend that all guests travel with a passport validity date of at least six months past the date of departure from the island and check specific country of origin requirements. For information on obtaining or renewing a US passport please visit the US Department of State. For information on UK passports, visit the HM Passport Office. In our current times, passports are the most expeditious and reliable form of identification when traveling domestically or internationally.
We strongly recommend travel insurance. We have a select partner with whom you can arrange insurance for your trip. Click here for more information.
When planning your Barbados vacation remember that the Barbados Dollar is converted at the rate of $1.98 BDS to $1.00 U.S. The Barbados Dollar is fixed to the U.S Dollar and does not fluctuate. Its rate to other currencies fluctuates daily based on their fluctuations relative to the U.S. Dollar. On your travels around Barbados you will find that US and UK currency is accepted across the island, and most stores and restaurants accept major credit cards and travellers cheques. Of course most Barbados hotels and other lodging also accept these various forms of payment.
Credit Cards and Travellers Cheques
Most Barbados hotels, restaurants and businesses will accept credit cards. Travelers cheques in U.S., U.K. and Canadian funds are also cashable at many outlets. There are many commercial banks in Barbados (mostly British and Canadian) and most have ATMs that will accept credit cards. They all dispense funds in Barbados Dollars at the current rate of exchange.
The major credit cards that are readily accepted are:
- Carte Blanche
- Diners Club
AMEX Not Accepted for Local Payments at The Club, Barbados. Due to ongoing issues with American Express credit card processing, please be advised that, until further notice, American Express credit cards cannot be used to pay local charges and incidentals at thehotel. Visa, MasterCard, and Discover are welcomed.
- Bring clothes for the tropics.
- Light cotton dresses and light jackets for formal wear.
- Casual slacks and lightweight shorts for the times when you are not in a bathing suit.
- Beachwear should also include sunglasses, sunblock and beach footwear.
Electricity in Barbados is: 110 volts/50 cycles.There is a reliable electricity supply, with only the occassional outage.
Standard plug types in Barbados are: (1) Flat blade (2 flat blades) and (2) Flat blades with round grounding pin. Most Barbados hotels can provide adapters and transformers for hair dryers and other appliances. Alternative energy: The island uses solar power mainly for hot-water systems.
Barbados Drivers License & Permit
You can obtain a Barbados drivers license on producing a valid National or International License. The cost for a visitors permit is US$5 for a 2-month permit and US$50 for a 1-year permit. All authorised car rental companies can issue you a drivers license if you have a valid license.
Illness & Allergies
The Resort is located in a tropical environment. Although the Resort does employ a year round pest control service, the Resort, its owners, employees, agents, insurers, and suppliers shall absolutely not be liable for any claim, injury or illness resulting from exposure to Zika virus, Chikungunya virus, or any other insect-borne virus or tropical illness whatsoever. The Resort’s food and beverage outlets use wholesome ingredients, which may include peanuts, tree nuts, seeds, shellfish, shrimp, seafood, soy, milk, wheat, and other potential allergens. Due to the large scale of the Resort’s food and beverage operations, the Resort cannot guarantee that food items will not come in contact with potential allergens even if a particular food does not normally include such ingredients. Guests with food allergies or dietary restrictions should be advised accordingly and take precautions. The Resort is not able to accommodate individually tailored meals for specific guests based on their individual dietary needs. The Resort and its owners, employees, agents, insurers, and suppliers shall absolutely not be liable for any claim, injury or illness resulting from food allergies or specific dietary needs or requirements. | <urn:uuid:a4889ff9-64b4-4c8a-9ec4-4d5be08e4ac2> | CC-MAIN-2017-47 | https://www.theclubbarbados.com/explore/travel-tips/ | 2017-11-20T01:57:37Z | s3://commoncrawl/crawl-data/CC-MAIN-2017-47/segments/1510934805894.15/warc/CC-MAIN-20171120013853-20171120033853-00678.warc.gz | en | 0.915668 | 876 | [
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Next week the SearchNetworking team will be reporting to you from Interop in Las Vegas. If my email inbox is any indication, there’s a lot going on at the show this year. I’ve received at least 500 emails over the last three weeks from vendors who desperately want me to tell you their stories.
There’s only so much ink in my pen, so you won’t hear all those stories. I apologize for that. But we’ll be bringing you as many interesting bits of news on this show as we can.
As a favor to you, our readers, we’ve reached out to some of the experts who will be speaking at Interop this year to get their insight on what to expect from the show. I caught up with Anne Skamarock, research director at Focus Consulting, who will be taking the stage several times to talk about virtualization. I asked her to tell us what to look for on the virtualization front at Interop.
Tessa Parmenter chatted with wireless networking expert Lisa Phifer about WLAN topics at Interop, Kate Dostart talked mobile apps and devices with Farpoint Group’s Craig Mathias, and Kara Gattine tracked down Burton Group’s Paul DeBeasi and picked his brain about enterprise wireless deployments.
We hope this whets your appetite for Interop news. We’ll be publishing news, blog posts and video from this show. Keep an eye on our Interop Las Vegas 2008 special news coverage page for all the news that’s fit to print. | <urn:uuid:20b86d24-cb4e-46e1-b9fb-5d4344dc8956> | CC-MAIN-2017-47 | http://itknowledgeexchange.techtarget.com/networkhub/what-happens-at-interop-doesnt-stay-in-vegas/ | 2017-11-20T21:47:30Z | s3://commoncrawl/crawl-data/CC-MAIN-2017-47/segments/1510934806225.78/warc/CC-MAIN-20171120203833-20171120223833-00678.warc.gz | en | 0.943706 | 330 | [
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Philippine statement delivered by Ambassador Teddy Locsin Jr. at the Trusteeship Council Chamber, United Nations Headquarters, New York, on July 24, 2017.
DURING negotiations for the 2030 Agenda, we fought strongly for the recognition of the positive contribution of migrants to inclusive growth and sustainable development and, frankly and flat out, to our consumption driven economy.
The absence of a specific Sustainable Development Goal on migration might be said to highlight the crosscutting nature or wide beneficial ramifications of migration to both home and destination countries. We don’t like to think of it as oversight. We like to think that migration is so deeply embedded in, or linked to all of the 17 SDGs, that there is no need for a category of their own.
At the national level, migrants are also deeply integrated into our development plan. We know we cannot make growth more inclusive, lower poverty incidence, create more jobs, make individuals and communities more resilient, drive innovation and build greater trust in the government and in society1 if we do not address the needs and harness the potentials of our 10 million overseas Filipinos.
And so we have, over the past 40 years, mainstreamed migration. We have developed laws, policies and programs that address all aspects and phases of migration, from labor and economic migration to migration in crises. We have implemented a migrant-centered approach to the entire migration cycle, from pre-employment, transit, on-site employment and eventual return and reintegration programs.
But this is not solely a domestic issue. Bilateral and regional frameworks are essential. In 2015 we advanced the migration agenda with the New York Declaration2. The Global Compact on Migration needs to do more. It must examine the barriers to maximizing the positive contributions of migrants, lay down actionable commitments, specific targets and put in place a governance and monitoring framework, and address the roles of local authorities. And it must go beyond the perception
that migrants contribute to development only through financial remittances and equally look at their “social” remittances.
We call migrant workers partners in development. They are more. They are drivers of the progress we have achieved. Let us not leave
1 From President Duterte’s foreword to the Philippine Development Agenda 2017-2022: “Through this plan, we will empower the poor and marginalized, push for improved transparency and accountability in governance and fuel our economy. Specifically, we intend to make growth more inclusive by lowering poverty incidence in the rural areas, creating more jobs, making individuals and communities more resilient, driving innovation and building greater trust in the government
and in society.”
2 New York Declaration on Migrants and Refugees, adopted on September 26, 2015. | <urn:uuid:2f3c3c4b-d366-475d-9b74-fbf4b946e2e9> | CC-MAIN-2017-47 | https://businessmirror.com.ph/the-linkages-between-migration-and-development/ | 2017-11-20T21:02:15Z | s3://commoncrawl/crawl-data/CC-MAIN-2017-47/segments/1510934806225.78/warc/CC-MAIN-20171120203833-20171120223833-00678.warc.gz | en | 0.925189 | 554 | [
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At the estate sale that I wrote about previously, I brought home very little stuff stuff but mostly magazines and catalogs. The catalogs make me the happiest.
That's nearly six thousand pages of eye candy right there.
Can you see Pip, the ancient paper eater in the background? She's circling like a hungry shark.
I can't say that I've ever seen a sale with old catalogs and I never see them in antique stores - so I was stoked out of my mind to see these in the basement. This is from two days of visiting the basement but I wish that I'd just sucked it up and hauled more out of there that first day because there were LOADS more of them. My back said "no" but my heart said "yes" and honestly, I'd worked up the kind of sweat that most people associate with a junkie trying to get a monkey off of their back. That's not the reputation that you want in the estate sale circuit.
All of the catalogs are from Sears and most are from the 50's and 60's with one being from the 80's. I've never known anyone to keep catalogs that far back. It kind of blew my mind. My grandmother told me that people never kept catalogs back in her day because they used them for toilet paper in the outhouses! Totally true but I guess that explains where most of the old, old ones went! Straight down the crapper.
I've been fighting the urge to look through every single one of them and it's really hard. I wish that you all could be here with me to flip through the pages and crow over the contents. I thought I'd share some of the pages with you here.
And I can't resist including this one:
And this little flashback to the 80's.
Tube tops and sailor caps!
These catalogs make me super happy to look through but oh my gosh, I have to admit that I mostly just want to find myself a time machine and zoom back to go shopping! Wouldn't it be wild if I filled out one of the order cards, sent it in and a few weeks later, the items actually arrived after being pulled from some kind of magical space-time continuum stockroom somewhere? Sigh. A gal can dream.
And these pages do nothing to squelch my constant rant about the lack of color and pattern in home decor these days. I know: Whine. Whine. Whine.
Until next time,
x's and o's, | <urn:uuid:2eb1244f-03ff-410d-b606-f9fa970cbc60> | CC-MAIN-2017-47 | http://ranchdressingwithearthakitsch.blogspot.com.au/2012/09/catalog-mania.html | 2017-11-21T15:51:00Z | s3://commoncrawl/crawl-data/CC-MAIN-2017-47/segments/1510934806419.21/warc/CC-MAIN-20171121151133-20171121171133-00678.warc.gz | en | 0.977395 | 519 | [
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I just realized this is a brand new book. Well, a month and 18 days old. I reserved this after an email from Mystery One plugged it.
Set in 2000 (or so). Dexter is a outstanding HS hockey goalie in Pittsburgh. He bombs at the state championship game and sneaks out rather than risk the violent crowd. On his way home Dexter is kidnapped by fans of the opposing team and brutalized. Eight years later he has a lousy warehouse job, a cot in his pal's basement and has not spoken to his father in about six years.
Dexter works in a produce warehouse up the street from his father's store. One night he meets Lou, the co-owner of another produce warehouse who looks and smells like a bum. Lou pays Dexter $100 to take Lou back to Lou's packrat and dust encrusted home and bury Lou's dead, frozen cat. Later on Lou asks Dexter to find Lou's former fiancee, Agnes Zagbroski. Agnes is better known as Mercy Carnahan a famous film actress who disappeared and has been unfound for 50 years. Lou is soon to die of cancer and Dexter, compelled by Lou's dresser drawer full of hoarded cash decides to start looking.
Dexter is also motivated by a desire to mentally improve. He dropped out of college after two years and has been working a dead end job since. His weekends are mostly spent doped up so he can sleep straight through. He is very tightly wound in an attempt to keep back the panic attacks and dizzy spells that occur whenever he thinks of his abduction. Dexter will occasionally see masked faces in a crowd or hallucinate them attacking him.
Dexter hooks up with his new hot chick warehouse manager and heads to New York to investigate. Dexter looks. Dexter finds. Dexter vividly dreams of the femme fatale film version of Mercy. Dexter keeps looking and finding. Dexter fights his terrors. Hot Chick fails at her fears. Dexter faces fears. Dexter finds Agnes. Ending is mysterious - to me anyway.
1. This feels like first novel do-too-much-at-once-itis. Maybe I am being unfair but there is a lot of extra stuff in here. The Hot Chick Manager. Crazy guy and family in New Jersey. Dexter's loser pal. Sure, everything ties in and I like the book but I can complain all I want.
2. Ayoob makes much use of the Pittsburgh setting with geography, different neighborhoods, sporting culture, anti-fag/pussy/girlie attitudes in sports. I did not drive around the city much - that I recall - but getting around on those twisting two-laners that pass as highways there had me constantly confused about where I was.
3. Good job by Ayoob putting together the mental mix-up that is Dexter.
4. Ambiguity of ending SPOILER ALERT was odd to me. Dexter returns to Lou's house after Lou's death to collect the drawer full of cash promised to him by Lou. He finds the drawer empty and note signed by Mercy. Was the cash taken by Agnes? Dexter in a mental fog? The spirit of Mercy? I presume it was the spirit of Mercy who, after all, spoke to Agnes and Dexter both. But, I'm not sure about the whole ending because there was nothing supernatural style earlier.
5. How many people ask Ayoob if he is related to the gun writer?
6. A poster on Bill Crider's blog had a comment about a novel with an ambiguous ending and settled on the: "if he'd 'a wanted ya to know he'd 'a told ya" rule." | <urn:uuid:f589f575-6d0b-4e8e-bc08-38769ee11d50> | CC-MAIN-2017-47 | http://booksareforsquares.blogspot.com/2010_11_01_archive.html | 2017-11-22T11:03:02Z | s3://commoncrawl/crawl-data/CC-MAIN-2017-47/segments/1510934806569.66/warc/CC-MAIN-20171122103526-20171122123526-00678.warc.gz | en | 0.97635 | 758 | [
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Download the report in .pdf
Authors: M.H. Paapst (ICTRecht BV; University of Groningen); T. Mulder (University of Groningen)
- Overview of the project
The Media Pluralism Monitor (MPM) is a research tool that was designed to identify potential risks to media pluralism in the Member States of the European Union. This narrative report has been produced within the framework of the first pan-European implementation of the MPM. The implementation was conducted in 28 EU Member States, Montenegro and Turkey with the support of a grant awarded by the European Union to the Centre for Media Pluralism and Media Freedom (CMPF) at the European University Institute.
- Methodological note
The CMPF cooperated with experienced, independent national researchers to carry out the data collection and to author the narrative reports, except in the cases of Malta and Italy where data collection was carried out centrally by the CMPF team. The research was based on a standardised questionnaire and apposite guidelines that were developed by the CMPF.
In the Netherlands, the CMPF partnered with M.H. Paapst en T. Mulder, who conducted the data collection and commented the variables in the questionnaire and interviewed relevant experts. The report was reviewed by CMPF staff. Moreover, to ensure accurate and reliable findings, a group of national experts in each country reviewed the answers to particularly evaluative questions (see Annexe 2 for the list of experts).
Risks to media pluralism are examined in four main thematic areas, which represent the main areas of risk for media pluralism and media freedom: Basic Protection, Market Plurality, Political Independence and Social Inclusiveness. The results are based on the assessment of 20 indicators – five per each thematic area:
|Basic Protection||Market Plurality||Political Independence||Social Inclusiveness|
|Protection of freedom of expression||Transparency of media ownership||Political control over media outlets||Access to media for minorities|
|Protection of right to information||Media ownership concentration (horizontal)||Editorial autonomy
|Access to media for local/regional communities and for community media|
|Journalistic profession, standards and protection||Cross-media concentration of ownership and competition enforcement||Media and democratic electoral process||Access to media for people with disabilities|
|Independence and effectiveness of the media authority||Commercial & owner influence over editorial content||State regulation of resources and support to media sector||Access to media for women
|Universal reach of traditional media and access to the Internet||Media viability
|Independence of PSM governance and funding||Media literacy
The results for each area and indicator are presented on a scale from 0% to 100%. Scores between 0 and 33% are considered low risk, 34 to 66% are medium risk, while those between 67 and 100% are high risk. On the level of indicators, scores of 0 were rated 3% and scores of 100 were rated 97% by default, to avoid an assessment of total absence or certainty of risk.
Disclaimer: The content of the report does not necessarily reflect the views of the CMPF or the EC, but represents the views of the national country team that carried out the data collection and authored the report.
The Netherlands, a relatively small country in the north-west of Europe, has a little over 17 million inhabitants. The officially spoken language is Dutch. 22% of the inhabitants are originating from another country, of which 44% from Western countries and 56% from non-Western countries. The largest non-Western minority groups are the Turks (approx. 320.000) and the Surinamese (approx. 300.000), who are both not officially recognized as a national minority. The Frisians are the only minority recognized by law.
The representation of religious and philosophical groups is guaranteed by the Nederlandse Publieke Omroep (Dutch Public Broadcasting, NPO). The Dutch Media Authority issues national airtime to religious and philosophical broadcasting associations. The main broadcasting associations – NTR, EO, KRO-NCRV and VPRO/HUMAN – do take care of airtime targeted at religious and philosophical groups as of 1 January 2016.
The economy in the Netherlands is growing steadily, for the 8th quarter in a row. The political situation is stable but fragmented, with, just to illustrate, 28 different political parties taking part in the 2017 general elections.
There is a strong tradition in regard to freedom of the press, and the Dutch public media system is characterized by various associations that represent different societal groups. The journalistic culture in the Netherlands is characterized with freedom of speech, plurality and self-regulation and has a strong tradition concerning ethic codes and codes of conduct. Media owners could have political ties of any kind, but there are no signs of political control over the newspapers, radio or television channels.
The largest media outlets based on their audience share are:
De Telegraaf, Algemeen Dagblad (newspapers),
Radio 538, NPO Radio 3FM (radio channels),
NPO Nederland 1 and RTL4 (television channels).
For print media the largest publishers are TMG (Telegraaf Media Groep) en De Persgroep. The largest national and regional newspaper publishers have a partnership to collectively distribute their newspapers. The Holland Media Combinatie is making use of TMG Distributie for the physical distribution.
For radio and television the three largest distributors are Ziggo (33%), KPN (30%) and UPC (16%). Liberty Global owns UPC and took over Ziggo in 2015. After the consolidation that took place in 2016, Ziggo became the largest distributor in the Netherlands.
The assessment completed for this report was conducted between June and October 2016, thus not taking into account any new developments since then.
The authors wish to thank the panel of experts and consulted professionals, institutions and academics for providing information and insight.
The Netherlands scores low risk in the areas of Basic Protection, Social Inclusiveness and Political Independence, as can be seen in the figure below. As for Market Plurality the country scores medium risk.
For each of the domains, the results are discussed by indicator.
The Basic Protection indicators represent the regulatory backbone of the media sector in every contemporary democracy. They measure a number of potential areas of risk, including the existence and effectiveness of the implementation of regulatory safeguards for freedom of expression and the right to information; the status of journalists in each country, including their protection and ability to work; the independence and effectiveness of the national regulatory bodies that have competence to regulate the media sector; and the reach of traditional media and access to the Internet.
All indicators in the Basic Protection area indicate a low risk. This implies that the Netherlands have a solid legal framework for media pluralism. There are although some remarks that are worth mentioning.
Protection of freedom of expression scores a very low risk (6%). The legal protection of freedom of expression does not show any substantial problems. In the Dutch Constitution freedom of expression is guaranteed and no explicit restrictions are made. Restrictions can however be found in national laws and regulations such as the Dutch Penal Code. This also means it is easy to change these restrictions depending on the political parties ruling the country. If we look at the way freedom of expression is respected in practice, and look for indications that the laws safeguarding freedom of expression may be ineffective, we see that according to the Persvrijheidsmonitor 2015 there are some examples of minor incidents over the last three years. Still a low risk is indicated as these incidents are not considered common practice.
Protection of right to information also scores a low risk (13%). Along with the Freedom of expression, the Dutch Constitution gives people the right to receive information. For public information that right has explicitly been granted in the Act on the Openness of Governance (Wet Openbaarheid van Bestuur). The act regulates how individuals can get information on administrative matters in documents, held by public authorities or companies carrying out work for a public authority. Recently a new bill got approved by the House of Representatives that will replace the Act on Openness of Governance (Wet Openbaar Bestuur, WOB) with the Act Open Government (Wet open overheid). This bill still (October 2016) needs approval of the Senate.
There are no explicitly mentioned appeal mechanisms in Dutch law concerning protection of right to information, but based on administrative law, appeal can be filed against decisions of government bodies in general. Procedural rules for this are laid down in de General Act on Public Administration (Algemene wet bestuursrecht, article 7:1). The Courts of First Instance are competent for the complaints with regard to the right of access to information, and a complainant can go to the Council of State for an appeal decision.
The indicator on Journalistic profession, standards and protection scores a low risk, but on the border to medium (33%). Journalism is a free profession in the Netherlands. This means that everybody is able to call himself a journalist, there are no restrictions or legal requirements and no licensing is necessary to call yourself a journalist. This is one of the reasons that there are about 16.000 journalists in the Netherlands. Of these 16.000 journalist approximately 75% is unionized. The main reason why the indicator almost scores a medium risk is because of the current lack of regulatory safeguards for the protection of journalistic sources. Protection of journalistic sources is not enshrined in Dutch statutory law. In September 2014, the Dutch legislator issued two bills on the protection of journalistic sources. The bills follow several judgments against the Netherlands for violating Article 10 of the European Convention on Human Rights (Europees Verdrag voor de Rechten van de Mens) in cases concerning journalists and source protection. With three violations on the matter in the last seven years (Voskuil v. the Netherlands in 2007; Sanoma Uitgevers B.V. v. the Netherlands in 2010; and Telegraaf Media Nederland Landelijke Media B.V. and Others v. the Netherlands in 2012). The Dutch government has been repeatedly criticized by the European Court of Human Rights for not having legislation in place that legally guarantees the protection of journalistic sources. Therefore, with these new bills, source protection issues in the Netherlands will be regulated under two new laws. The process towards these new laws took quite some time and has been suspended for several reasons.
Independence and effectiveness of the media authority scores a low risk (5%). The Dutch media authority is called the Commissariaat van de Media. Board members of this authority are, according to article 7.4 Media Act, not allowed to: 1. have a membership in the House of Representatives (Tweede Kamer), the Senate (Eerste Kamer) and provincial or municipal administration 2. be employed within any ministry of an institution under the responsibility of a ministry 3. be employed with a public or commercial broadcasting, or relating institutions, or publisher. At the head of the authority are three board members (June 2016), which have been appointed by the Minister of Education, Culture and Science.
The minister can dismiss an individual board member, but only for well-defined reasons, which are specified in the law (e.g. incapacity, or because the board member requested it). We consider this to be a low risk, since the Minister had only two reasons to dismiss an individual board member and there were no early dismissals in the last years.
According to the last evaluation of the inner working of the authority, the Government only overruled two decisions during the period 1989 till 2011. This was done because the overruled decisions were not considered to be legally valid, or were not seen as taken in the national public interest. There are no recent cases known where decisions are being overruled by the government.
Universal reach of traditional media and access to the Internet is also a low risk indicator (13%). This value is due to the existence of laws that prescribe national coverage for the television channels of the Dutch Public broadcaster (NPO). Transmission should take place without any additional costs for the end user. According to telecommunications operator KPN, the DVBT network (Digitenne) can be received in almost the whole country (KPN, 2015). TV scan (2015) draws a similar conclusion It is also arranged by law that the Dutch Public broadcaster (NPO) has national coverage for its radio channels. According to fmscan.org (2016), the Dutch public broadcaster’s (NPO) main radio channels (these are NPO Radio 1, NPO Radio 2, NPO 3FM, NPO Radio 4) have a technical reach (via FM frequencies) all over the Netherlands. When we assess the broadband coverage in the country, a percentage of 99,99% of the population is covered. We considered this to be a low risk. The average internet connection speed is 18 Mbps. Assessing the ownership concentration of the ISPs in the country we conclude that in 2014, the three biggest ISPs together hold a 84% marketshare: KPN (40%), Ziggo (28%) and UPC (16%). In 2015 UPC and Ziggo merged into one company.
The Market Plurality indicators examine the existence and effectiveness of the implementation of transparency and disclosure provisions with regard to media ownership. In addition, they assess the existence and effectiveness of regulatory safeguards to prevent horizontal and cross-media concentration of ownership and the role of competition enforcement and State aid control in protecting media pluralism. Moreover, they seek to evaluate the viability of the media market under examination as well as whether and if so, to what extent commercial forces, including media owners and advertisers, influence editorial decision-making.
The indicator on transparency of media ownership scores a low risk (3%) The national law contains transparency and disclosure provisions that oblige both listed and non-listed media firms to disclose their ownership structures in records/documents that are accessible to the public, and to report ownership structures to the public authorities.
The indicator on media ownership concentration (horizontal) indicates a high risk in the Netherlands with 83%. Since 2011 the country does no longer have media legislation containing specific thresholds and/or other limitations that are based on objective criteria (e.g. number of licences, audience share, circulation, distribution of share capital or voting rights, turnover/revenue, etc.) in order to prevent a high degree of horizontal concentration of ownership in the media sector.
The Dutch Media Authorities’ Media Monitor also does not report market shares, only audience shares and financial figures are being reported for the main media groups. For a reliable calculation of market shares, segmented financial data of media groups is needed. This data cannot be found in all media group’s financial accounts.
Based on audience shares the indicators for the concentration of ownership in the radio, television and newspaper markets show a high risk, with joint audience shares of the four largest companies that are between 69% and 91%.
The indicator on Cross-media concentration of ownership and competition enforcement scores a medium risk (40%)
The Dutch media legislation does not contain specific limits to prevent a high level of cross-media concentration of ownership. When we look at the marketshare of the top 3 owners across different media markets we estimate that there is a concentration of 84,5%.
A high degree of (horizontal, vertical and/or cross-media) concentration can be prevented through the enforcement of competition rules, including rules on merger control, that take into account the specificities of the media sector. The Autoriteit Consument en Markt (Competition Authority, Authority of Consumer and Market) made the public interest as its explicit goal (ACM, 2013). There are examples of media mergers in which remedies are imposed based on issues such as consumer access (Van der Burg & Van den Bulck, 2015)
The risk related to ‘Commercial & owner influence over editorial content’ scores a low risk (4%) because there are self-regulatory instruments granting social protection to journalists in cases of changes in ownership or editorial line, and that ensure that decisions regarding appointments and dismissals of editors-in-chief are not influenced by commercial interests.
Editorial charters of newspapers are not a legal obligation but resulted from an agreement between newspaper publishers and the journalist union. The essence is strict separation of editorial and commercial responsibilities. The Media Act does not require commercial broadcasting to safeguard journalistic ethics in their editorial charter as it is mandatory for public broadcasting. So it is a result of self-regulatory instruments that the appointment and dismissal of editors-in-chief are not influenced by commercial interests.
The indicator on Media viability scores a medium risk (55%). In annual reports of the large traditional media organizations no indications can be found that these media organizations are developing sources of revenue other than traditional revenue streams. For viability this is considered a medium risk. There is no recent data available on the revenues of the audiovisual, radio, and newspaper publishing sector, so we do not know if the revenues increased over the past two years. However we do know that the expenditure for online advertising has not increased nor decreased during that period.
There are however some promising initiatives to develop sources of revenue other than traditional streams. For example there is the Correspondent, a daily, online and advert-free medium that has as a goal to bring news in context with other things that happen in the world. The Correspondent is only accessible online and started after a crowdfunding campaign. That started in September 2013 with 18.000 members and had over 45.000 members in 2016. The website is available in Dutch and some parts also in English. Another initiative is Blendle, they offer the possibility to buy news articles from newspapers and magazines. But instead of having to buy for example a complete newspaper, it is possible to buy just that one article that you are interested in. The initiative started in 2014 and at first it was only possible to read Dutch newspapers and one Dutch magazine, but this increased rapidly. Since 2015 German newspapers are included and Blendle aims to offer more international newspapers in the future. But as said above, these are all new initiatives, from new organisations.
The Political Independence indicators assess the existence and effectiveness of regulatory safeguards against political bias and political control over the media outlets, news agencies and distribution networks. They are also concerned with the existence and effectiveness of self-regulation in ensuring editorial independence. Moreover, they seek to evaluate the influence of the State (and, more generally, of political power) over the functioning of the media market and the independence of public service media.
Three out of five indicators in the Political Independence area score low risk. In particular, State regulation of resources and support to the media sector and Independence of PSM governance and funding score very low, at 3%. Two indicators score medium risk, with Political control over the media outlets at the highest of 54%.
Largely due to the lack of related regulation the indicator Political control over media outlets scores a medium risk (54%). The press is fully unregulated in the Netherlands. The Dutch press policy is based on the ‘free market’ thinking. This also means that there are no laws that regulate possible conflict of interests between owners of media and the ruling parties, partisan groups or politicians. In practice, there are nevertheless no cases of conflict found with regards to media ownership and holding government office. The law also does not contain limitations to direct and indirect control of audiovisual media, radio stations, newspapers, news agencies and media distribution networks by parties, partisan groups or politicians. Nevertheless, none of the largest news agencies or other larger types of media are dependent on political groupings in terms of ownership, affiliation of key personnel or editorial policy.
The indicator on Editorial autonomy scores a low level of risk (25%). There are no regulatory safeguards (e.g. law, statute) that prevent political influence over the appointments and dismissals of editors-in-chief, however there is no evidence of systematic political interference concerning appointments and dismissals of editors-in-chief. The journalistic culture in the Netherlands is characterised with freedom of speech, plurality and self-regulation and has a strong tradition concerning ethic codes and codes of conduct. The Press Council (Raad voor de Journalistiek) is the self-regulatory body for media. Codes of conduct are laid down in the Press Council’s guideline (Leidraad voor de Journalistiek). In addition to the Press Council’s codes of conduct, some media outlets also have their own codes of conduct or other accountability instruments. For example, the public broadcasting association for news and information (NOS), has its own ombudsman for complaints. The largest media outlets in terms of audience are De Telegraaf, Algemeen Dagblad (newspapers), Radio 538, NPO Radio 3FM (radio channels), NPO Nederland 1 and RTL4 (television channels). Apart from De Telegraaf, all media outlets are member of the Press Council. RTL4 is not licensed in the Netherlands but in Luxembourg, and it has to comply with Luxembourg jurisdiction. Nevertheless, RTL’s news broadcast has an editorial charter and recognises the Press Council’s code of conduct via this charter.
The indicator on Media and democratic electoral process scores medium (44%). No specific rules exist about the participation of political actors in the Dutch public service media’s (NPO’s) news and information programmes. The different codes of conduct are discussed in the NPO’s handbook for radio and television but no specific reference is made to political actors. As editorial charters of news and information programmes of the (national) broadcasting associations are not publicly available, it is not possible to verify whether specific reference is made to political actors in these programmes. This contributes the assessment of risk for this indicator.
The indicator on State regulation of resources and support to the media sector scores a very low risk (3%) because of the existence and effective implementation of regulations that ensure a fair and transparent distribution of state advertisements and subsidies, as well as spectrum allocation.
The indicator on Independence of PSM governance and funding is also at low risk (3% ). The law provides a fair and transparent appointment procedure for management and board functions in PSM, which guarantees independence from government or other political influence. The Dutch Public Broadcaster’s (NPO’s) board of directors is appointed, suspended or dismissed by the supervisory board with the minister’s approval, according to article 2.8 Media Act 2008. Since appointment, suspension and dismissal are being done by the supervisory board, the minister’s approval is only considered to be a formality. The composition of and appointment procedures of the supervisory board are described in article 2.5 of the Media Act 2008, and the appointment for a period of at least five years is done by the minister. There is no evidence of systematic conflicts concerning appointments and dismissals.
The Social Inclusiveness indicators are concerned with access to media by various groups in society The indicators assess regulatory and policy safeguards for community media, and for access to media by minorities, local and regional communities, women and people with disabilities. In addition to access to media by specific groups, the media literacy context is important for the state of media pluralism. The Social Inclusiveness area therefore also examines the country’s media literacy environment, as well as the digital skills of the overall population.
On average, the Netherlands score low risk on Social Inclusiveness. There are however two indicators which score medium risk: Access to media for people with disabilities and Access to media for women, with respectively 50% and 44%.
The indicator on access to media for minorities scores a low risk (3%). The law guarantees access to airtime on PSM channels to minorities. The Commissariaat voor de Media (Dutch Media Authority, CvdM) issues national airtime to religious and philosophical broadcasting associations (so-called ‘2.42 broadcasts’), article 2.42 Media Act 2008. The access is proportional to the size of their populations in the country, without any significant exception.
The indicator on Access to media for local/regional communities and for community media scores a low risk (25%)
Although the law grants regional, local and community media access to media platforms, and all 12 provinces of the Netherlands do have regional public media services, there is some cause for concern. According to article 2.170b Media Act 2008 local public service media receive subsidy of 1,30 euro per household in the respective community, this is the responsibility of municipalities. In her evaluation of these local PSM, the Dutch Media Authority concludes that not all municipalities’ subsidies meet the requirements (to little is paid) and one third of the local PSM’s financial situation is concerning (CvdM, 2013 p. 15).
The indicator Access to media for people with disabilities scores medium risk (50%) because the policies in place are nascent and the measures taken are fragmented. On the one hand the Media Act 2008 enforces both public and commercial broadcasters, as well as on-demand media services, to provide for subtitles for television content. However, on the other hand it took the Dutch government almost ten years to go ahead with the ratification of the UN treaty Convention on the Rights of Persons with Disabilities, making the Netherlands one of the last countries in Europe that needs to change its laws in order to uphold and protect the rights of persons with disabilities as enshrined in the UN Convention. Because of the recent ratification, the Act on equal treatment on the grounds of disability or chronic illness will be changed and there will become an obligation for the media to gradually ensure access to media content by people with disabilities, unless this obligation would be considered to be disproportionate to enact for the media. The law does not give any real certainty for people with disabilities, and it is very unclear if people can actually demand access to media content and services from large media corporations. Compared to broadcasters such as the BBC, it is evident that the Dutch policy is underdeveloped: until very recently audio description for blind people was not even available on TV. Just recently (November 2015) the PSM started with making audio descriptions available for one TV series, and in 2016 for six telemovies. The biggest private TV channel RTL4 only started in January 2016 with audio description for one popular TV series. The second biggest private TV channel SBS6 has got no audio descriptions available.
It is noted by one of the experts that we should monitor the possibility of accessing the internet in the next couple of years: Accessing the Internet is considered to be a growing problem for the visually disabled. Although speech software can in theory be used to navigate the Internet, Internet sites increasingly use plugins and formats that thwart the use of such software. Websites should have a simple layout, so it is easier for visually disabled to use speech software.
The indicator on Access to media for women scores a medium risk (44%). There is an equal rights law that also applies to employment of women in media organizations (de Wet gelijke behandeling van mannen en vrouwen). The “College voor de rechten van de mens” is the Dutch equality body monitoring compliance with the law. Although the College does not have sanctioning/enforcement powers, its advices are being followed in 81% of all cases they handle. The Dutch courts are also obliged to consider the advice of the College if a case is taken to court. The College also has the legal right to go to court and ask a judge to forbid a certain behaviour, or to reverse the consequences of an illicit behaviour. The College has never used this right because of satisfaction with the current effectiveness of the regulatory safeguards. According to the College, the equal rights law is implemented effectively.
However, the indicator on Access to media for women still scores medium risk. This is mainly due to the fact that the PSM has a gender equality policy, which is limited in scope, and also outdated. It also lacks concrete and measurable ambitions. According to the CvdM the situation is actually worse than in 2010. (See:http://www.cvdm.nl/nieuws/npo-haalt-afspraken-maar-nog-steeds-te-weinig-vrouwen-op-televisie/)
The indicator on ‘Media literacy’ is at low risk (6%). There is a well-developed policy and already a strong tradition of policy making in this area. The existing measures are coherent and up-to-date with the latest societal changes. The Dutch government has acknowledged that learning to become media wise is an essential condition for all citizens in order to participate in a multimedia society (McGonagle & Schumacher 2014, p. 38)
Based on the findings of the MPM2016, the following two issues have been identified by the country team as more pressing or deserving particular attention by policy-makers in order to promote media pluralism and media freedom in the country:
- The high concentration of media ownership
- The lack of transparency of media concentration
The results of the Media Pluralism Monitor indicate that only in the area of Basic Protection all indicators score low risk. Within all the three other areas there are some medium risks noted, and in the Market Plurality one indicator scores a high risk: the main risk to media plurality in the Netherlands is the high concentration of media ownership. The law does not offer specific thresholds and limits so as to prevent the concentration.
However, based on the current data a stricter anti-concentration regulation cannot be recommended because the small market size could actually be causing this concentration.
There is also some concern about the lack of reliable data as to market share by revenue. Although audience share figures are readily available for television, radio and newspapers, reliable figures for market share by revenue are not. It is recommended that the transparency of media concentration should be enhanced, and that this lack of transparency is addressed by the government by imposing information duties and information rights on the television, radio and newspaper actors.
The Country team is composed of one or more national researchers that carried out the data collection and authored the country report.
|First name||Last name||Position||Institution||MPM2016 CT Leader (please indicate with X)|
|Mathieu||Paapst||Researcher||University of Groningen||X|
|Trix||Mulder||Research-assistant||University of Groningen|
The Group of Experts is composed of specialists with a substantial knowledge and experience in the field of media. The role of the Group of Experts was to review especially sensitive/subjective evaluations drafted by the Country Team in order to maximize the objectivity of the replies given, ensuring the accuracy of the final results.
|First name||Last name||Position||Institution|
|Thomas||Bruning||Representative of a journalist organisation||The Dutch Association of Journalists|
|Piet||Bakker||Academic/NGO researchers on social/political/cultural issues related to the media||Hogeschool Utrecht|
|Herman||Wolswinkel||Representative of a publisher organisation||NDP|
|Hans||Van Kranenburg||Academic/NGO researcher in media law and/or economics||Radboud University|
|Edmund||Lauf||Representative of media regulators||Dutch media authority|
|Bart||Brouwers||Academic/NGO researchers on social/political/cultural issues related to the media||University of Groningen|
|Babette||Aalberts||Academic/NGO researcher in media law and/or economics||Vrije Universiteit Amsterdam|
|Anne-Lieke||Mol||Representative of a broadcaster organisation||NPO|
For more information on MPM methodology, see the CMPF report “Monitoring Media Pluralism in Europe: Application of the Media Pluralism Monitor 2016 in EU-28, Montenegro and Turkey”, http://monitor.cmpf.eui.eu/
Persvrijheidsmonitor 2015 -Volgenant, O. M. B. J., & McGonagle, T. – 2016.
Evaluatie Commissariaat voor de Media 2007-2011 [Evaluation of the Dutch Media Authority 2007-2011] -DSP-groep – 2013, http://www.rijksoverheid.nl/bestanden/documenten-en-publicaties/rapporten/2013/09/17/evaluatie-commissariaat-voor-de-media-2007-2011/evaluatie-commissariaat-voor-de-media-2007-2011.pdf
Evaluation of the funding of local public media institutions in the years 2009-2012 -Commissariaat voor de Media – 2013, http://www.cvdm.nl/wp-content/uploads/2013/12/evaluatie-van-de-financiering-van-de-lokale-publieke-media-instellingen-in-de-jaren-2009-2012.pdf
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Fitting climax to 15 years of inspiring young players at grassroots level
Rugby School are enjoying their moment in the limelight after director of sport Simon Brown was crowned Rugby Coach of the Year at the prestigious Crabbie’s National Rugby Awards at Twickenham.
Held at England rugby’s headquarters, the National Rugby Awards celebrate the best of English rugby, from grassroots to the very pinnacle of the game.
Brown, former director of boys’ games, had been shortlisted for the award in recognition of his dedicated service since joining the School 15 years ago.
Brown leaves the role having played an instrumental part in Rugby’s first unbeaten season since 1978 last year, following more than a decade of hard work nurturing players on and off the field.
And the coach admits winning the award was a fitting climax to many years of inspiring the young generation at grassroots level.
“It’s incredibly humbling,” said Brown.
“I didn’t expect to come away with this especially when there are so many good quality people – particularly in the grassroots level – that do so much and get so may rewards from it.
“Grassroots is integral to what everybody does. That’s where I started, at the grassroots level, and you just repeat the things that people did for you.
“I think that’s one of the great things about rugby football. You try and replicate that to future generations and anything from the game that you’re passionate about and enjoy immensely.”
And the rugby enthusiast revealed he was taken aback by the scale of the awards, which were held at the iconic location of Twickenham, where former England captain and event ambassador Chris Robshaw was on hand to congratulate winners.
“I looked on the website to see what the NRAs were all about and I had no idea it would be of this magnitude,” said Brown.
“The awards embody everything about the game, the atmosphere of the game and all the presentations – it’s a brilliant event.”
Brown, who has produced several junior internationalists including Northampton Saints’ Harry Mallinder, has been at the heart of running countless rugby sevens programmes and prep school matches throughout his 15-year stint at the co-educational independent school.
And he insisted that Rugby School would always have a special place in his heart and paid tribute to the inclusive ethos that the school embodies when encouraging youngsters to play the game.
“The history about the place is what makes this school so special,” he said.
“We’re custodians of the sport and it was the birthplace of the game. One of the things we do is give as many opportunities as we can to people who want to come and visit the school, whether that’s from overseas of people from local schools.
“People have the opportunity to come and have a fantastic education whatever they’re back ground and that’s one of the great things about Rugby School.”
# Crabbie’s Alcoholic Ginger Beer passionately support rugby at all levels. To help amateur clubs thrive, they have launched Crabbie’s Rugby Rewards. Find out how your club can earn kit for every bottle of Crabbie’s sold in your clubhouse at www.crabbiesrugby.co.uk | <urn:uuid:906ad823-bf5c-4638-b600-20e0e0e62d71> | CC-MAIN-2017-47 | http://www.rugbyadvertiser.co.uk/sport/rugby-union/rugby-national-award-for-former-rugby-school-coach-simon-brown-1-8147377 | 2017-11-22T10:47:17Z | s3://commoncrawl/crawl-data/CC-MAIN-2017-47/segments/1510934806569.66/warc/CC-MAIN-20171122103526-20171122123526-00678.warc.gz | en | 0.97425 | 708 | [
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I've had a love/hate relationship with the Bride of Christ most of my life. In the times I have needed her most, I have felt failed by her, and in the times I have felt myself stray far from her, she has pursued and loved me. These are strange words to use about an entity, a full body of individuals, imperfect men and women stumbling through life and the Bible as clearly as they can, but they are true words. There is nothing on earth I love more than the Church.
I have felt her failings near and I have chased her down in desperation—and there is no other place I would rather commune, break bread and share wine, than within her haven.
Ephesians 4 speaks of building the unity of the Church and oh how that resonates.
To see a whole body purified, strengthened, and grown into full maturity, ready to be presented to Christ—this I love.
And so I'm grateful that I've been asked to contribute monthly to a publication that pulls from every fold of her robes, every particle of her skin, and every joint and marrow, to build up and unify the Church as best we can with our earth encrusted words.
My first column is up today:
I pass a field of blackbirds every morning on my way from class to work. There are a thousand of them wide in a Texas spread and I can’t stop trying to count them with my mind. Thirty of them are perched on a shrub close to the ground, but its branches do not bend or weep. I marvel at its strength. I marvel at the lightness of the birds, all thirty of them.
This desert shrub carries the weight of the blackest birds and I think of Jeremiah 17 while I drive. Continue reading... | <urn:uuid:bcc471e6-6198-4dca-aac3-b3c2a5c4dccf> | CC-MAIN-2017-47 | http://www.sayable.net/blog/2012/09/deeper-church-thirty-blackbirds-or-more | 2017-11-22T11:03:03Z | s3://commoncrawl/crawl-data/CC-MAIN-2017-47/segments/1510934806569.66/warc/CC-MAIN-20171122103526-20171122123526-00678.warc.gz | en | 0.981686 | 370 | [
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Keeping your clubs in order can be chaotic at the best of times, so why not sort them out in one sudden strike with Callaway Golf’s new Strike Stand Bag? It’s light, small and spacious enough for all your accessories, while a colour coordinated divider system puts your irons, woods, hybrids, putter and driver in their place. The Callaway Golf Mens Strike Stand Bag is a lightweight stand bag available in black, blue or orange.
Strike Stand Bag Eight Way Divider Management System
Callaway has been wise to colour coordinate their Strike Stand Bag’s eight way divider system for convenient club management. The rear area is designed to hold your driver and 3 wood, while the three full length slots take care of your irons and the other 4 mesh covered areas are ideal for your hybrids, putters and other clubs.
Callaway Golf Strike Stand Bag Features
Padded for comfort and designed for convenience, Callaway Golf’s Strike Stand Bag has a wealth of features to improve the organisation and speed of your game. Perfect planning makes perfect players.
Colour Coordinated Eight Way Divider & Four Full Length Dividers
Six Zippered Pockets
Dual Strap System & Dual Stand System
Included Rain Hood & Umbrella Holder
Callaway Golf Dual Strap & Dual Stand Systems
Supporting your clubs from above and below is a combination of systems for maximum comfort and stability. A heavily padded Callaway Golf Dual Strap System distributes weight evenly across the shoulders for a comfortable carry, while a standard Dual Stand System keeps the full bag upright and securely stores away with Velcro strap retainers. The Callaway Golf Mens Strike Stand Bag also features six pockets including a ball pocket and cooler pocket, umbrella holder and clip on rain hood. | <urn:uuid:a2228604-6720-4c9e-98f4-757670f46438> | CC-MAIN-2017-47 | http://www.eaglesgolfonline.co.uk/ourshop/prod_101082-Callaway-Golf-Strike-Stand-Bag.html | 2017-11-23T05:09:56Z | s3://commoncrawl/crawl-data/CC-MAIN-2017-47/segments/1510934806736.55/warc/CC-MAIN-20171123050243-20171123070243-00678.warc.gz | en | 0.887548 | 368 | [
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The Trypanosoma cruzi acute infections acquired in infancy and childhood seem asymptomatic, but approximately one third of the chronically infected cases show Chagas disease up to three decades or later. Autoimmunity and parasite persistence are competing theories to explain the pathogenesis of Chagas disease 1, 2. To separate roles played by parasite persistence and autoimmunity in Chagas disease we inoculate the T. cruzi in the air chamber of fertilized eggs. The mature chicken immune system is a tight biological barrier against T. cruzi and the infection is eradicated upon development of its immune system by the end of the first week of growth 3. The chicks are parasite-free at hatching, but they retain integrated parasite mitochondrial kinetoplast DNA (kDNA) minicircle within their genome that are transferred to their progeny. Documentation of the kDNA minicircle integration in the chicken genome was obtained by a targeted prime TAIL-PCR, Southern hybridizations, cloning, and sequencing 3, 4. The kDNA minicircle integrations rupture open reading frames for transcription and immune system factors, phosphatase (GTPase), adenylate cyclase and phosphorylases (PKC, NF-Kappa B activator, PI-3K) associated with cell physiology, growth, and differentiation 3, 5-7, and other gene functions. Severe myocarditis due to rejection of target heart fibers by effectors cytotoxic lymphocytes is seen in the kDNA mutated chickens, showing an inflammatory cardiomyopathy similar to that seen in human Chagas disease. Notably, heart failure and skeletal muscle weakness are present in adult chickens with kDNA rupture of the dystrophin gene in chromosome 1 8. Similar genotipic alterations are associated with tissue destruction carried out by effectors CD45+, CD8γδ+, CD8α lymphocytes. Thus this protozoan infection can induce genetically driven autoimmune disease.
20 Related JoVE Articles!
Adenoviral Transduction of Naive CD4 T Cells to Study Treg Differentiation
Institutions: Helmholtz Zentrum München.
Regulatory T cells (Tregs) are essential to provide immune tolerance to self as well as to certain foreign antigens. Tregs can be generated from naive CD4 T cells in vitro
with TCR- and co-stimulation in the presence of TGFβ and IL-2. This bears enormous potential for future therapies, however, the molecules and signaling pathways that control differentiation are largely unknown.
Primary T cells can be manipulated through ectopic gene expression, but common methods fail to target the most important naive state of the T cell prior to primary antigen recognition. Here, we provide a protocol to express ectopic genes in naive CD4 T cells in vitro
before inducing Treg differentiation. It applies transduction with the replication-deficient adenovirus and explains its generation and production. The adenovirus can take up large inserts (up to 7 kb) and can be equipped with promoters to achieve high and transient overexpression in T cells. It effectively transduces naive mouse T cells if they express a transgenic Coxsackie adenovirus receptor (CAR). Importantly, after infection the T cells remain naive (CD44low
) and resting (CD25-
) and can be activated and differentiated into Tregs similar to non-infected cells. Thus, this method enables manipulation of CD4 T cell differentiation from its very beginning. It ensures that ectopic gene expression is already in place when early signaling events of the initial TCR stimulation induces cellular changes that eventually lead into Treg differentiation.
Immunology, Issue 78, Cellular Biology, Molecular Biology, Medicine, Biomedical Engineering, Bioengineering, Infection, Genetics, Microbiology, Virology, T-Lymphocytes, Regulatory, CD4-Positive T-Lymphocytes, Regulatory, Adenoviruses, Human, MicroRNAs, Antigens, Differentiation, T-Lymphocyte, Gene Transfer Techniques, Transduction, Genetic, Transfection, Adenovirus, gene transfer, microRNA, overexpression, knock down, CD4 T cells, in vitro differentiation, regulatory T cell, virus, cell, flow cytometry
Isolation and Analysis of Brain-sequestered Leukocytes from Plasmodium berghei ANKA-infected Mice
Institutions: The Walter and Eliza Hall Institute of Medical Research.
We describe a method for isolation and characterization of adherent inflammatory cells from brain blood vessels of P. berghei
ANKA-infected mice. Infection of susceptible mouse-strains with this parasite strain results in the induction of experimental cerebral malaria, a neurologic syndrome that recapitulates certain important aspects of Plasmodium falciparum
-mediated severe malaria in humans 1,2
. Mature forms of blood-stage malaria express parasitic proteins on the surface of the infected erythrocyte, which allows them to bind to vascular endothelial cells. This process induces obstructions in blood flow, resulting in hypoxia and haemorrhages 3
and also stimulates the recruitment of inflammatory leukocytes to the site of parasite sequestration.
Unlike other infections, i.e neutrotopic viruses4-6
, both malaria-parasitized red blood cells (pRBC) as well as associated inflammatory leukocytes remain sequestered within blood vessels rather than infiltrating the brain parenchyma. Thus to avoid contamination of sequestered leukocytes with non-inflammatory circulating cells, extensive intracardial perfusion of infected-mice prior to organ extraction and tissue processing is required in this procedure to remove the blood compartment. After perfusion, brains are harvested and dissected in small pieces. The tissue structure is further disrupted by enzymatic treatment with Collagenase D and DNAse I. The resulting brain homogenate is then centrifuged on a Percoll gradient that allows separation of brain-sequestered leukocytes (BSL) from myelin and other tissue debris. Isolated cells are then washed, counted using a hemocytometer and stained with fluorescent antibodies for subsequent analysis by flow cytometry.
This procedure allows comprehensive phenotypic characterization of inflammatory leukocytes migrating to the brain in response to various stimuli, including stroke as well as viral or parasitic infections. The method also provides a useful tool for assessment of novel anti-inflammatory treatments in pre-clinical animal models.
Immunology, Issue 71, Infection, Infectious Diseases, Pathology, Hematology, Molecular Biology, Cellular Biology, Mouse, Brain, Intravascular inflammation, leukocytes, Plasmodium berghei, parasite, malaria, animal model, flow cytometry
Recurrent Herpetic Stromal Keratitis in Mice, a Model for Studying Human HSK
Institutions: Saint Louis University.
Herpetic eye disease, termed herpetic stromal keratitis (HSK), is a potentially blinding infection of the cornea that results in over 300,000 clinical visits each year for treatment. Between 1 and 2 percent of those patients with clinical disease will experience loss of vision of the infected cornea. The vast majority of these cases are the result of reactivation of a latent infection by herpes simplex type I virus and not due to acute disease. Interestingly, the acute infection is the model most often used to study this disease. However, it was felt that a recurrent model of HSK would be more reflective of what occurs during clinical disease. The recurrent animal models for HSK have employed both rabbits and mice. The advantage of rabbits is that they experience reactivation from latency absent any known stimulus. That said, it is difficult to explore the role that many immunological factors play in recurrent HSK because the rabbit model does not have the immunological and genetic resources that the mouse has. We chose to use the mouse model for recurrent HSK because it has the advantage of there being many resources available and also we know when reactivation will occur because reactivation is induced by exposure to UV-B light. Thus far, this model has allowed those laboratories using it to define several immunological factors that are important to this disease. It has also allowed us to test both therapeutic and vaccine efficacy.
Infection, Issue 70, Immunology, Virology, Medicine, Infectious Diseases, Ophthalmology, Herpes, herpetic stromal keratitis, HSK, keratitis, pathogenesis, clinical evaluation, virus, eye, mouse, animal model
A Simple and Rapid Protocol to Non-enzymatically Dissociate Fresh Human Tissues for the Analysis of Infiltrating Lymphocytes
Institutions: Université Libre de Bruxelles, Université Libre de Bruxelles, Université Libre de Bruxelles, Université Libre de Bruxelles.
The ability of malignant cells to evade the immune system, characterized by tumor escape from both innate and adaptive immune responses, is now accepted as an important hallmark of cancer. Our research on breast cancer focuses on the active role that tumor infiltrating lymphocytes play in tumor progression and patient outcome. Toward this goal, we developed a methodology for the rapid isolation of intact lymphoid cells from normal and abnormal tissues in an effort to evaluate them proximate to their native state. Homogenates prepared using a mechanical dissociator show both increased viability and cell recovery while preserving surface receptor expression compared to enzyme-digested tissues. Furthermore, enzymatic digestion of the remaining insoluble material did not recover additional CD45+
cells indicating that quantitative and qualitative measurements in the primary homogenate likely genuinely reflect infiltrating subpopulations in the tissue fragment. The lymphoid cells in these homogenates can be easily characterized using immunological (phenotype, proliferation, etc.
) or molecular (DNA, RNA and/or protein) approaches. CD45+
cells can also be used for subpopulation purification, in vitro
expansion or cryopreservation. An additional benefit of this approach is that the primary tissue supernatant from the homogenates can be used to characterize and compare cytokines, chemokines, immunoglobulins and antigens present in normal and malignant tissues. This protocol functions extremely well for human breast tissues and should be applicable to a wide variety of normal and abnormal tissues.
Immunology, Issue 94, Tumor immunology, tumor infiltrating lymphocytes, CD45+, breast cancer, fresh tissue homogenate, non-enzymatic dissociation, primary tissue supernatant
In Vitro Assay to Evaluate the Impact of Immunoregulatory Pathways on HIV-specific CD4 T Cell Effector Function
Institutions: The Ragon Institute of MGH, MIT and Harvard, Centre de Recherche du Centre Hospitalier de l'Université de Montréal (CRCHUM).
T cell exhaustion is a major factor in failed pathogen clearance during chronic viral infections. Immunoregulatory pathways, such as PD-1 and IL-10, are upregulated upon this ongoing antigen exposure and contribute to loss of proliferation, reduced cytolytic function, and impaired cytokine production by CD4 and CD8 T cells. In the murine model of LCMV infection, administration of blocking antibodies against these two pathways augmented T cell responses. However, there is currently no in vitro
assay to measure the impact of such blockade on cytokine secretion in cells from human samples. Our protocol and experimental approach enable us to accurately and efficiently quantify the restoration of cytokine production by HIV-specific CD4 T cells from HIV infected subjects.
Here, we depict an in vitro
experimental design that enables measurements of cytokine secretion by HIV-specific CD4 T cells and their impact on other cell subsets. CD8 T cells were depleted from whole blood and remaining PBMCs were isolated via Ficoll separation method. CD8-depleted PBMCs were then incubated with blocking antibodies against PD-L1 and/or IL-10Rα and, after stimulation with an HIV-1 Gag peptide pool, cells were incubated at 37 °C, 5% CO2
. After 48 hr, supernatant was collected for cytokine analysis by beads arrays and cell pellets were collected for either phenotypic analysis using flow cytometry or transcriptional analysis using qRT-PCR. For more detailed analysis, different cell populations were obtained by selective subset depletion from PBMCs or by sorting using flow cytometry before being assessed in the same assays. These methods provide a highly sensitive and specific approach to determine the modulation of cytokine production by antigen-specific T-helper cells and to determine functional interactions between different populations of immune cells.
Immunology, Issue 80, Virus Diseases, Immune System Diseases, HIV, CD4 T cell, CD8 T cell, antigen-presenting cell, Cytokines, immunoregulatory networks, PD-1: IL-10, exhaustion, monocytes
Using Fluorescent Proteins to Monitor Glycosome Dynamics in the African Trypanosome
Institutions: Clemson University Eukaryotic Pathogens Innovation Center.
is a kinetoplastid parasite that causes human African trypanosomiasis (HAT), or sleeping sickness, and a wasting disease, nagana, in cattle1
. The parasite alternates between the bloodstream of the mammalian host and the tsetse fly vector. The composition of many cellular organelles changes in response to these different extracellular conditions2-5
Glycosomes are highly specialized peroxisomes in which many of the enzymes involved in glycolysis are compartmentalized. Glycosome composition changes in a developmental and environmentally regulated manner4-11
. Currently, the most common techniques used to study glycosome dynamics are electron and fluorescence microscopy; techniques that are expensive, time and labor intensive, and not easily adapted to high throughput analyses.
To overcome these limitations, a fluorescent-glycosome reporter system in which enhanced yellow fluorescent protein (eYFP) is fused to a peroxisome targeting sequence (PTS2), which directs the fusion protein to glycosomes12
, has been established. Upon import of the PTS2eYFP fusion protein, glycosomes become fluorescent. Organelle degradation and recycling results in the loss of fluorescence that can be measured by flow cytometry. Large numbers of cells (5,000 cells/sec) can be analyzed in real-time without extensive sample preparation such as fixation and mounting. This method offers a rapid way of detecting changes in organelle composition in response to fluctuating environmental conditions.
Infectious Diseases, Issue 90, glycosomes, trypanosomes, flow cytometry, kinetoplastids, fluorescent protein, peroxisomes
Collection, Isolation, and Flow Cytometric Analysis of Human Endocervical Samples
Institutions: University of Manitoba, University of Manitoba.
Despite the public health importance of mucosal pathogens (including HIV), relatively little is known about mucosal immunity, particularly at the female genital tract (FGT). Because heterosexual transmission now represents the dominant mechanism of HIV transmission, and given the continual spread of sexually transmitted infections (STIs), it is critical to understand the interplay between host and pathogen at the genital mucosa. The substantial gaps in knowledge around FGT immunity are partially due to the difficulty in successfully collecting and processing mucosal samples. In order to facilitate studies with sufficient sample size, collection techniques must be minimally invasive and efficient. To this end, a protocol for the collection of cervical cytobrush samples and subsequent isolation of cervical mononuclear cells (CMC) has been optimized. Using ex vivo
flow cytometry-based immunophenotyping, it is possible to accurately and reliably quantify CMC lymphocyte/monocyte population frequencies and phenotypes. This technique can be coupled with the collection of cervical-vaginal lavage (CVL), which contains soluble immune mediators including cytokines, chemokines and anti-proteases, all of which can be used to determine the anti- or pro-inflammatory environment in the vagina.
Medicine, Issue 89, mucosal, immunology, FGT, lavage, cervical, CMC
Isolation and Physiological Analysis of Mouse Cardiomyocytes
Institutions: Vanderbilt University, Vanderbilt University.
Cardiomyocytes, the workhorse cell of the heart, contain exquisitely organized cytoskeletal and contractile elements that generate the contractile force used to pump blood. Individual cardiomyocytes were first isolated over 40 years ago in order to better study the physiology and structure of heart muscle. Techniques have rapidly improved to include enzymatic digestion via coronary perfusion. More recently, analyzing the contractility and calcium flux of isolated myocytes has provided a vital tool in the cellular and sub-cellular analysis of heart failure. Echocardiography and EKGs provide information about the heart at an organ level only. Cardiomyocyte cell culture systems exist, but cells lack physiologically essential structures such as organized sarcomeres and t-tubules required for myocyte function within the heart. In the protocol presented here, cardiomyocytes are isolated via Langendorff perfusion. The heart is removed from the mouse, mounted via the aorta to a cannula, perfused with digestion enzymes, and cells are introduced to increasing calcium concentrations. Edge and sarcomere detection software is used to analyze contractility, and a calcium binding fluorescent dye is used to visualize calcium transients of electrically paced cardiomyocytes; increasing understanding of the role cellular changes play in heart dysfunction. Traditionally used to test drug effects on cardiomyocytes, we employ this system to compare myocytes from WT mice and mice with a mutation that causes dilated cardiomyopathy. This protocol is unique in its comparison of live cells from mice with known heart function and known genetics. Many experimental conditions are reliably compared, including genetic or environmental manipulation, infection, drug treatment, and more. Beyond physiologic data, isolated cardiomyocytes are easily fixed and stained for cytoskeletal elements. Isolating cardiomyocytes via perfusion is an extremely versatile method, useful in studying cellular changes that accompany or lead to heart failure in a variety of experimental conditions.
Cellular Biology, Issue 91, cardiomyocyte isolation, Langendorff, contractility, calcium transients
In Situ Detection of Autoreactive CD4 T Cells in Brain and Heart Using Major Histocompatibility Complex Class II Dextramers
Institutions: University of Nebraska, Lincoln, University of Nebraska, Lincoln, University of Nebraska, Lincoln.
This report demonstrates the use of major histocompatibility complex (MHC) class II dextramers for detection of autoreactive CD4 T cells in situ
in myelin proteolipid protein (PLP) 139-151-induced experimental autoimmune encephalomyelitis (EAE) in SJL mice and cardiac myosin heavy chain-α (Myhc) 334-352-induced experimental autoimmune myocarditis (EAM) in A/J mice. Two sets of cocktails of dextramer reagents were used, where dextramers+
cells were analyzed by laser scanning confocal microscope (LSCM): EAE, IAs
/PLP 139-151 dextramers (specific)/anti-CD4 and IAs
/Theiler’s murine encephalomyelitis virus (TMEV) 70-86 dextramers (control)/anti-CD4; and EAM, IAk
/Myhc 334-352 dextramers/anti-CD4 and IAk
/bovine ribonuclease (RNase) 43-56 dextramers (control)/anti-CD4. LSCM analysis of brain sections obtained from EAE mice showed the presence of cells positive for CD4 and PLP 139-151 dextramers, but not TMEV 70-86 dextramers suggesting that the staining obtained with PLP 139-151 dextramers was specific. Likewise, heart sections prepared from EAM mice also revealed the presence of Myhc 334-352, but not RNase 43-56-dextramer+
cells as expected. Further, a comprehensive method has also been devised to quantitatively analyze the frequencies of antigen-specific CD4 T cells in the ‘Z’ serial images.
Immunology, Issue 90, dextramers; MHC class II; in situ; EAE; brain; EAM; heart; confocal microscopy.
Cecal Ligation Puncture Procedure
Institutions: Temple University , Temple University .
Human sepsis is characterized by a set of systemic reactions in response to intensive and massive infection that failed to be locally contained by the host. Currently, sepsis ranks among the top ten causes of mortality in the USA intensive care units 1
. During sepsis there are two established haemodynamic phases that may overlap. The initial phase (hyperdynamic) is defined as a massive production of proinflammatory cytokines and reactive oxygen species by macrophages and neutrophils that affects vascular permeability (leading to hypotension), cardiac function and induces metabolic changes culminating in tissue necrosis and organ failure. Consequently, the most common cause of mortality is acute kidney injury. The second phase (hypodynamic) is an anti-inflammatory process involving altered monocyte antigen presentation, decreased lymphocyte proliferation and function and increased apoptosis. This state known as immunosuppression or immune depression sharply increases the risk of nocosomial infections and ultimately, death. The mechanisms of these pathophysiological processes are not well characterized. Because both phases of sepsis may cause irreversible and irreparable damage, it is essential to determine the immunological and physiological status of the patient. This is the main reason why many therapeutic drugs have failed. The same drug given at different stages of sepsis may be therapeutic or otherwise harmful or have no effect 2,3
. To understand sepsis at various levels it is crucial to have a suitable and comprehensive animal model that reproduces the clinical course of the disease. It is important to characterize the pathophysiological mechanisms occurring during sepsis and control the model conditions for testing potential therapeutic agents.
To study the etiology of human sepsis researchers have developed different animal models. The most widely used clinical model is cecal ligation and puncture (CLP). The CLP model consists of the perforation of the cecum allowing the release of fecal material into the peritoneal cavity to generate an exacerbated immune response induced by polymicrobial infection. This model fulfills the human condition that is clinically relevant. As in humans, mice that undergo CLP with fluid resuscitation show the first (early) hyperdynamic phase that in time progresses to the second (late) hypodynamic phase. In addition, the cytokine profile is similar to that seen in human sepsis where there is increased lymphocyte apoptosis (reviewed in 4,5
). Due to the multiple and overlapping mechanisms involved in sepsis, researchers need a suitable sepsis model of controlled severity in order to obtain consistent and reproducible results.
Medicine, Issue 51, sepsis, systemic inflammation, infection, septic shock, animal model
Directed Differentiation of Induced Pluripotent Stem Cells towards T Lymphocytes
Institutions: Pennsylvania State University College of Medicine.
Adoptive cell transfer (ACT) of antigen-specific CD8+
cytotoxic T lymphocytes (CTLs) is a promising treatment for a variety of malignancies 1
. CTLs can recognize malignant cells by interacting tumor antigens with the T cell receptors (TCR), and release cytotoxins as well as cytokines to kill malignant cells. It is known that less-differentiated and central-memory-like (termed highly reactive
) CTLs are the optimal population for ACT-based immunotherapy, because these CTLs have a high proliferative potential, are less prone to apoptosis than more differentiated cells and have a higher ability to respond to homeostatic cytokines 2-7
. However, due to difficulties in obtaining a high number of such CTLs from patients, there is an urgent need to find a new approach to generate highly reactive Ag-specific CTLs for successful ACT-based therapies.
TCR transduction of the self-renewable stem cells for immune reconstitution has a therapeutic potential for the treatment of diseases 8-10
. However, the approach to obtain embryonic stem cells (ESCs) from patients is not feasible. Although the use of hematopoietic stem cells (HSCs) for therapeutic purposes has been widely applied in clinic 11-13
, HSCs have reduced differentiation and proliferative capacities, and HSCs are difficult to expand in in vitro
cell culture 14-16
. Recent iPS cell technology and the development of an in vitro
system for gene delivery are capable of generating iPS cells from patients without any surgical approach. In addition, like ESCs, iPS cells possess indefinite proliferative capacity in vitro
, and have been shown to differentiate into hematopoietic cells. Thus, iPS cells have greater potential to be used in ACT-based immunotherapy compared to ESCs or HSCs.
Here, we present methods for the generation of T lymphocytes from iPS cells in vitro
, and in vivo
programming of antigen-specific CTLs from iPS cells for promoting cancer immune surveillance. Stimulation in vitro
with a Notch ligand drives T cell differentiation from iPS cells, and TCR gene transduction results in iPS cells differentiating into antigen-specific T cells in vivo
, which prevents tumor growth. Thus, we demonstrate antigen-specific T cell differentiation from iPS cells. Our studies provide a potentially more efficient approach for generating antigen-specific CTLs for ACT-based therapies and facilitate the development of therapeutic strategies for diseases.
Stem Cell Biology, Issue 63, Immunology, T cells, induced pluripotent stem cells, differentiation, Notch signaling, T cell receptor, adoptive cell transfer
Isolation and Th17 Differentiation of Naïve CD4 T Lymphocytes
Institutions: The University of Florida.
Th17 cells are a distinct subset of T cells that have been found to produce interleukin 17 (IL-17), and differ in function from the other T cell subsets including Th1, Th2, and regulatory T cells. Th17 cells have emerged as a central culprit in overzealous inflammatory immune responses associated with many autoimmune disorders. In this method we purify T lymphocytes from the spleen and lymph nodes of C57BL/6 mice, and stimulate purified CD4+ T cells under control and Th17-inducing environments. The Th17-inducing environment includes stimulation in the presence of anti-CD3 and anti-CD28 antibodies, IL-6, and TGF-β. After incubation for at least 72 hours and for up to five days at 37 °C, cells are subsequently analyzed for the capability to produce IL-17 through flow cytometry, qPCR, and ELISAs. Th17 differentiated CD4+CD25- T cells can be utilized to further elucidate the role that Th17 cells play in the onset and progression of autoimmunity and host defense. Moreover, Th17 differentiation of CD4+CD25- lymphocytes from distinct murine knockout/disease models can contribute to our understanding of cell fate plasticity.
Immunology, Issue 79, Cellular Biology, Molecular Biology, Medicine, Infection, Th17 cells, IL-17, Th17 differentiation, T cells, autoimmunity, cell, isolation, culture
Trichuris muris Infection: A Model of Type 2 Immunity and Inflammation in the Gut
Institutions: University of British Columbia, University of British Columbia.
is a natural pathogen of mice and is biologically and antigenically similar to species of Trichuris
infect humans and livestock1
. Infective eggs are given by oral gavage, hatch in the distal small intestine, invade the intestinal epithelial cells (IECs)
that line the crypts of the cecum and proximal colon and upon maturation the worms release eggs into the environment1
. This model is a powerful tool to
examine factors that control CD4+
T helper (Th) cell activation as well as changes in the intestinal epithelium. The immune response that occurs in
resistant inbred strains, such as C57BL/6 and BALB/c, is characterized by Th2 polarized cytokines (IL-4, IL-5 and IL-13) and expulsion of worms while Th1-associated
cytokines (IL-12, IL-18, IFN-γ) promote chronic infections in genetically susceptible AKR/J mice2-6
. Th2 cytokines promote physiological changes in
the intestinal microenvironment including rapid turnover of IECs, goblet cell differentiation, recruitment and changes in epithelial permeability and smooth muscle
contraction, all of which have been implicated in worm expulsion7-15
. Here we detail a protocol for propagating Trichuris muris
eggs which can
be used in subsequent experiments. We also provide a sample experimental harvest with suggestions for post-infection analysis. Overall, this protocol will provide
researchers with the basic tools to perform a Trichuris muris
mouse infection model which can be used to address questions pertaining to Th proclivity in
the gastrointestinal tract as well as immune effector functions of IECs.
Infection, Issue 51, Trichuris muris, mouse, Th2, intestine, inflammation
In vivo Imaging of Transgenic Leishmania Parasites in a Live Host
Institutions: University of Iowa, and the VA Medical Center, University of Iowa, and the VA Medical Center, University of Iowa, Washington University School of Medicine, Harbor-UCLA Medical Center, Hanley-Hardison Research Center, Iowa City VA Medical Center, University of Iowa.
Distinct species of Leishmania,
a protozoan parasite of the family Trypanosomatidae
, typically cause different human disease manifestations. The most common forms of disease are visceral leishmaniasis (VL) and cutaneous leishmaniasis (CL). Mouse models of leishmaniasis are widely used, but quantification of parasite burdens during murine disease requires mice to be euthanized at various times after infection. Parasite loads are then measured either by microscopy, limiting dilution assay, or qPCR amplification of parasite DNA. The in vivo
imaging system (IVIS) has an integrated software package that allows the detection of a bioluminescent signal associated with cells in living organisms. Both to minimize animal usage and to follow infection longitudinally in individuals, in vivo models for imaging Leishmania
spp. causing VL or CL were established. Parasites were engineered to express luciferase, and these were introduced into mice either intradermally or intravenously. Quantitative measurements of the luciferase driving bioluminescence of the transgenic Leishmania
parasites within the mouse were made using IVIS. Individual mice can be imaged multiple times during longitudinal studies, allowing us to assess the inter-animal variation in the initial experimental parasite inocula, and to assess the multiplication of parasites in mouse tissues. Parasites are detected with high sensitivity in cutaneous locations. Although it is very likely that the signal (photons/second/parasite) is lower in deeper visceral organs than the skin, but quantitative comparisons of signals in superficial versus deep sites have not been done. It is possible that parasite numbers between body sites cannot be directly compared, although parasite loads in the same tissues can be compared between mice. Examples of one visceralizing species (L. infantum chagasi
) and one species causing cutaneous leishmaniasis (L. mexicana
) are shown. The IVIS procedure can be used for monitoring and analyzing small animal models of a wide variety of Leishmania
species causing the different forms of human leishmaniasis.
Microbiology, Issue 41, IVIS, Leishmania, in vivo imaging, parasite, transgenic, bioluminescence, luciferase, cutaneous leishmaniasis, visceral leishmaniasis
An Experimental Model to Study Tuberculosis-Malaria Coinfection upon Natural Transmission of Mycobacterium tuberculosis and Plasmodium berghei
Institutions: University Hospital Heidelberg, Research Center Borstel.
Coinfections naturally occur due to the geographic overlap of distinct types of pathogenic organisms. Concurrent infections most likely modulate the respective immune response to each single pathogen and may thereby affect pathogenesis and disease outcome. Coinfected patients may also respond differentially to anti-infective interventions. Coinfection between tuberculosis as caused by mycobacteria and the malaria parasite Plasmodium
, both of which are coendemic in many parts of sub-Saharan Africa, has not been studied in detail. In order to approach the challenging but scientifically and clinically highly relevant question how malaria-tuberculosis coinfection modulate host immunity and the course of each disease, we established an experimental mouse model that allows us to dissect the elicited immune responses to both pathogens in the coinfected host. Of note, in order to most precisely mimic naturally acquired human infections, we perform experimental infections of mice with both pathogens by their natural routes of infection, i.e.
aerosol and mosquito bite, respectively.
Infectious Diseases, Issue 84, coinfection, mouse, Tuberculosis, Malaria, Plasmodium berghei, Mycobacterium tuberculosis, natural transmission
Sublingual Immunotherapy as an Alternative to Induce Protection Against Acute Respiratory Infections
Institutions: Universidad de la República, Trinity College Dublin.
Sublingual route has been widely used to deliver small molecules into the bloodstream and to modulate the immune response at different sites. It has been shown to effectively induce humoral and cellular responses at systemic and mucosal sites, namely the lungs and urogenital tract. Sublingual vaccination can promote protection against infections at the lower and upper respiratory tract; it can also promote tolerance to allergens and ameliorate asthma symptoms. Modulation of lung’s immune response by sublingual immunotherapy (SLIT) is safer than direct administration of formulations by intranasal route because it does not require delivery of potentially harmful molecules directly into the airways. In contrast to intranasal delivery, side effects involving brain toxicity or facial paralysis are not promoted by SLIT. The immune mechanisms underlying SLIT remain elusive and its use for the treatment of acute lung infections has not yet been explored. Thus, development of appropriate animal models of SLIT is needed to further explore its potential advantages.
This work shows how to perform sublingual administration of therapeutic agents in mice to evaluate their ability to protect against acute pneumococcal pneumonia. Technical aspects of mouse handling during sublingual inoculation, precise identification of sublingual mucosa, draining lymph nodes and isolation of tissues, bronchoalveolar lavage and lungs are illustrated. Protocols for single cell suspension preparation for FACS analysis are described in detail. Other downstream applications for the analysis of the immune response are discussed. Technical aspects of the preparation of Streptococcus pneumoniae
inoculum and intranasal challenge of mice are also explained.
SLIT is a simple technique that allows screening of candidate molecules to modulate lungs’ immune response. Parameters affecting the success of SLIT are related to molecular size, susceptibility to degradation and stability of highly concentrated formulations.
Medicine, Issue 90, Sublingual immunotherapy, Pneumonia, Streptococcus pneumoniae, Lungs, Flagellin, TLR5, NLRC4
A Mouse Model for Pathogen-induced Chronic Inflammation at Local and Systemic Sites
Institutions: Boston University School of Medicine, Boston University School of Medicine.
Chronic inflammation is a major driver of pathological tissue damage and a unifying characteristic of many chronic diseases in humans including neoplastic, autoimmune, and chronic inflammatory diseases. Emerging evidence implicates pathogen-induced chronic inflammation in the development and progression of chronic diseases with a wide variety of clinical manifestations. Due to the complex and multifactorial etiology of chronic disease, designing experiments for proof of causality and the establishment of mechanistic links is nearly impossible in humans. An advantage of using animal models is that both genetic and environmental factors that may influence the course of a particular disease can be controlled. Thus, designing relevant animal models of infection represents a key step in identifying host and pathogen specific mechanisms that contribute to chronic inflammation.
Here we describe a mouse model of pathogen-induced chronic inflammation at local and systemic sites following infection with the oral pathogen Porphyromonas gingivalis
, a bacterium closely associated with human periodontal disease. Oral infection of specific-pathogen free mice induces a local inflammatory response resulting in destruction of tooth supporting alveolar bone, a hallmark of periodontal disease. In an established mouse model of atherosclerosis, infection with P. gingivalis
accelerates inflammatory plaque deposition within the aortic sinus and innominate artery, accompanied by activation of the vascular endothelium, an increased immune cell infiltrate, and elevated expression of inflammatory mediators within lesions. We detail methodologies for the assessment of inflammation at local and systemic sites. The use of transgenic mice and defined bacterial mutants makes this model particularly suitable for identifying both host and microbial factors involved in the initiation, progression, and outcome of disease. Additionally, the model can be used to screen for novel therapeutic strategies, including vaccination and pharmacological intervention.
Immunology, Issue 90,
Pathogen-Induced Chronic Inflammation; Porphyromonas gingivalis; Oral Bone Loss; Periodontal Disease; Atherosclerosis; Chronic Inflammation; Host-Pathogen Interaction; microCT; MRI
A Parasite Rescue and Transformation Assay for Antileishmanial Screening Against Intracellular Leishmania donovani Amastigotes in THP1 Human Acute Monocytic Leukemia Cell Line
Institutions: University of Mississippi, University of Mississippi.
Leishmaniasis is one of the world's most neglected diseases, largely affecting the poorest of the poor, mainly in developing countries. Over 350 million people are considered at risk of contracting leishmaniasis, and approximately 2 million new cases occur yearly1
. Leishmania donovani
is the causative agent for visceral leishmaniasis (VL), the most fatal form of the disease. The choice of drugs available to treat leishmaniasis is limited 2
;current treatments provide limited efficacy and many are toxic at therapeutic doses. In addition, most of the first line treatment drugs have already lost their utility due to increasing multiple drug resistance 3
. The current pipeline of anti-leishmanial drugs is also severely depleted. Sustained efforts are needed to enrich a new anti-leishmanial drug discovery pipeline, and this endeavor relies on the availability of suitable in vitro
and axenic amastigotes assays5
are primarily used for anti-leishmanial drug screening however, may not be appropriate due to significant cellular, physiological, biochemical and molecular differences in comparison to intracellular amastigotes. Assays with macrophage-amastigotes models are considered closest to the pathophysiological conditions of leishmaniasis, and are therefore the most appropriate for in vitro
screening. Differentiated, non-dividing human acute monocytic leukemia cells (THP1) (make an attractive) alternative to isolated primary macrophages and can be used for assaying anti-leishmanial activity of different compounds against intracellular amastigotes.
Here, we present a parasite-rescue and transformation assay with differentiated THP1 cells infected in vitro
with Leishmania donovani
for screening pure compounds and natural products extracts and determining the efficacy against the intracellular Leishmania
amastigotes. The assay involves the following steps: (1) differentiation of THP1 cells to non-dividing macrophages, (2) infection of macrophages with L. donovani
metacyclic promastigotes, (3) treatment of infected cells with test drugs, (4) controlled lysis of infected macrophages, (5) release/rescue of amastigotes and (6) transformation of live amastigotes to promastigotes. The assay was optimized using detergent treatment for controlled lysis of Leishmania
-infected THP1 cells to achieve almost complete rescue of viable intracellular amastigotes with minimal effect on their ability to transform to promastigotes. Different macrophage:promastigotes ratios were tested to achieve maximum infection. Quantification of the infection was performed through transformation of live, rescued Leishmania
amastigotes to promastigotes and evaluation of their growth by an alamarBlue fluorometric assay in 96-well microplates. This assay is comparable to the currently-used microscopic, transgenic reporter gene and digital-image analysis assays. This assay is robust and measures only the live intracellular amastigotes compared to reporter gene and image analysis assays, which may not differentiate between live and dead amastigotes. Also, the assay has been validated with a current panel of anti-leishmanial drugs and has been successfully applied to large-scale screening of pure compounds and a library of natural products fractions (Tekwani et al.
Infection, Issue 70, Immunology, Infectious Diseases, Molecular Biology, Cellular Biology, Pharmacology, Leishmania donovani, Visceral Leishmaniasis, THP1 cells, Drug Screening, Amastigotes, Antileishmanial drug assay
Using Eggs from Schistosoma mansoni as an In vivo Model of Helminth-induced Lung Inflammation
Institutions: University of Pennsylvania , University of Pennsylvania .
parasites are blood flukes that infect an estimated 200 million people worldwide 1
. In chronic infection with Schistosoma
, the severe pathology, including liver fibrosis and splenomegaly, is caused by the immune response to the parasite eggs rather than the parasite itself 2
. Parasite eggs induce a Th2 response characterized by the production of IL-4, IL-5 and IL-13, the alternative activation of macrophages and the recruitment of eosinophils. Here, we describe injection of Schistosoma mansoni
eggs as a model to examine parasite-specific Th2 cytokine responses in the lung and draining lymph nodes, the formation of pulmonary granulomas surrounding the egg, and airway inflammation.
Following intraperitoneal sensitization and intravenous challenge, S. mansoni
eggs are transported to the lung via the pulmonary arteries where they are trapped within the lung parenchyma by granulomas composed of lymphocytes, eosinophils and alternatively activated macrophages 3-6
. Associated with granuloma formation, inflammation in the broncho-alveolar spaces, expansion of the draining lymph nodes and CD4 T cell activation can be observed. Here we detail the protocol for isolating Schistosoma mansoni
eggs from infected livers (modified from 7
), sensitizing and challenging mice, and recovering the organs (broncho-alveolar lavage (BAL), lung and draining lymph nodes) for analysis. We also include representative histologic and immunologic data and suggestions for additional immunologic analysis.
Overall, this method provides an in vivo
model to investigate helminth-induced immunologic responses in the lung, which is broadly applicable to the study of Th2 inflammatory diseases including helminth infection, fibrotic diseases, allergic inflammation and asthma. Advantages of this model for the study of type 2 inflammation in the lung include the reproducibility of a potent Th2 inflammatory response in the lung and draining lymph nodes, the ease of assessment of inflammation by histologic examination of the granulomas surrounding the egg, and the potential for long-term storage of the parasite eggs.
Immunology, Issue 64, Infection, Microbiology, helminth, parasite, mouse, Th2, lung, inflammation, granuloma, alternative activation, macrophage
Building a Better Mosquito: Identifying the Genes Enabling Malaria and Dengue Fever Resistance in A. gambiae and A. aegypti Mosquitoes
Institutions: Johns Hopkins University.
In this interview, George Dimopoulos focuses on the physiological mechanisms used by mosquitoes to combat Plasmodium falciparum and dengue virus infections. Explanation is given for how key refractory genes, those genes conferring resistance to vector pathogens, are identified in the mosquito and how this knowledge can be used to generate transgenic mosquitoes that are unable to carry the malaria parasite or dengue virus.
Cellular Biology, Issue 5, Translational Research, mosquito, malaria, virus, dengue, genetics, injection, RNAi, transgenesis, transgenic | <urn:uuid:d951abb5-6ffe-42aa-93f1-fa8cd829d929> | CC-MAIN-2017-47 | https://www.jove.com/visualize/abstract/23776551/analysis-dynamics-infiltrating-cd4-t-cell-subsets-heart-during | 2017-11-23T05:56:27Z | s3://commoncrawl/crawl-data/CC-MAIN-2017-47/segments/1510934806736.55/warc/CC-MAIN-20171123050243-20171123070243-00678.warc.gz | en | 0.903573 | 9,594 | [
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|line||Firmly builded with rafters of oak, the house of the farmer|
Stood on the side of a hill commanding the sea; and a shady
Sycamore grew by the door, with a woodbine wreathing around it.
|85||Rudely carved was the porch, with seats beneath; and a footpath|
Led through an orchard wide, and disappeared in the meadow.
Under the sycamore-tree were hives overhung by a penthouse,
Such as the traveller sees in regions remote by the roadside,
Built o'er a box for the poor, or the blessed image of Mary.
|90||Farther down, on the slope of the hill, was the well with its moss-grown|
Bucket, fastened with iron, and near it a trough for the horses.
Shielding the house from storms, on the north, were the barns and the farmyard.
There stood the broad-wheeled wains and the antique ploughs and the harrows;
There were the folds for the sheep; and there, in his feathered seraglio,
|95||Strutted the lordly turkey, and crowed the cock, with the selfsame|
Voice that in ages of old had startled the penitent Peter.
Bursting with hay were the barns, themselves a village. In each one
Far o'er the gable projected a roof of thatch; and a staircase,
Under the sheltering eaves, led up to the odorous cornloft.
|100||There too the dove-cot stood, with its meek and innocent inmates|
Murmuring ever of love; while above in the variant breezes
Numberless noisy weathercocks rattled and sang of mutation. | <urn:uuid:44570654-e242-42a4-aa93-d740cd7a343a> | CC-MAIN-2017-47 | http://seiyaku.com/lit/longfellow/evangeline/04.html | 2017-11-23T23:49:38Z | s3://commoncrawl/crawl-data/CC-MAIN-2017-47/segments/1510934807044.45/warc/CC-MAIN-20171123233821-20171124013821-00678.warc.gz | en | 0.96623 | 386 | [
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I think she might have something affecting her computer, like a virus. Any suggestions on what to do. And also what might be causing my computer to something lag in getting a response when selecting a website?
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Licensed to: Geeks to Go, Inc. | <urn:uuid:9f710065-a556-4807-8934-1851e3e42a32> | CC-MAIN-2017-47 | http://www.geekstogo.com/forum/topic/88414-internet-runs-very-slow/ | 2017-11-24T00:15:05Z | s3://commoncrawl/crawl-data/CC-MAIN-2017-47/segments/1510934807044.45/warc/CC-MAIN-20171123233821-20171124013821-00678.warc.gz | en | 0.937546 | 180 | [
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Last Saturday I taught a canning class sponsored by North Coast Opportunities at the Willits Grange. We had 10 people come out and spent four fun hours cooking and canning 54 jars of applesauce and green tomato pickles. It was awesome.
I emphasized two things:
- The food we were canning would otherwise have rotted on the ground.
- Canning is not as scary as people think it is.
I wrote a two-part series for Eat Mendocino about preserving food. First I talked about making refrigerator pickles, and then I explained how to preserve them using a water bath. I’m not on Facebook, but apparently there was some outcry that I had not followed the proper steps for food safety (which, for the record, I did).
While I appreciate that people want to avoid introducing bacteria into their canned food, I also think our fear-based societal inclinations prohibit us from doing a lot of perfectly safe activities. Back in the day, people didn’t even bother with waterbathing or pressure canning! My grandma still makes shelf-stable apple butter without putting it through a waterbath, and although I personally take that extra step, she has never killed or made a single member of her family sick using this method. My mom says she has a two-year-old jar of that apple butter in her pantry, and at Christmas I won’t hesitate to open it up and spread it on a piece of toast. And if there happens to be mold on the top of it, well… that’s a bummer, but it’s easy to spot and we won’t eat it.
Use your eyes and your nose. If it looks good and smells good, it’s good. I recently invented the statistic that you’re more likely to die from a shark attack than botulism. If you’re one of those people who doesn’t go in the ocean for fear of sharks, then maybe canning isn’t for you. But for the rest of you, food preservation is a fun, easy, and relatively risk-free way to keep the flavors of summer alive in the winter months. | <urn:uuid:68b65853-98cf-431d-98b6-a288671ab4b3> | CC-MAIN-2017-47 | https://myukiah.wordpress.com/2013/10/17/sharing-the-joy-of-canning/ | 2017-11-24T00:04:03Z | s3://commoncrawl/crawl-data/CC-MAIN-2017-47/segments/1510934807044.45/warc/CC-MAIN-20171123233821-20171124013821-00678.warc.gz | en | 0.971324 | 459 | [
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It is important that you inspect your shipment thoroughly before accepting and signing for your merchandise; after you sign for the merchandise you are responsible for filing all claims with the shipper. Do not assume that your product is not damaged just because the box and packaging is intact. You must check for both internal and external damage before you accept delivery. If you notice your product is damaged, refuse the package and note the damage on the bill of lading. Please call our customer service department immediately at 1-800-891-0896 and inform us of the damage. After you sign for the package you are responsible for damages. Please make sure that you inspect all packages before you sign for them.
If you receive a shipment from an order that you placed with eFaucets.com with obvious or concealed freight damage, refuse the shipment and note the damage on the bill of lading. We will then order and ship a replacement for you. We will charge you for the replacement at the originally quoted price and credit you upon the return of the original. If you sign for the shipment without reporting any damage you are responsible for all damages and must make any claims with the shipper. Please make sure that you inspect all packages before you sign for them.
If you fail to report concealed or obvious freight damage to the carrier prior to signing for the shipment, neither neither you or we have any recourse. | <urn:uuid:6670d477-1761-4e2a-8856-559dc97bf1c7> | CC-MAIN-2017-47 | https://www.efaucets.com/damaged.asp | 2017-11-24T18:49:07Z | s3://commoncrawl/crawl-data/CC-MAIN-2017-47/segments/1510934808742.58/warc/CC-MAIN-20171124180349-20171124200349-00678.warc.gz | en | 0.955417 | 282 | [
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A massive haul of drugs, initially thought to be cocaine worth R300m, was discovered in an Overberg town. Police said 253 boxes containing cocaine (which was later identified as heroin) were discovered on a farm in Villierdorp on June 21 2017.
The drugs were discovered in boxes meant for wine, intended for export, on the Eerste Hoop farm in the Western Cape South Africa.
Three suspects were arrested. One, Mark Rodrigues, 23, who is a Dutch national, remains in custody and is expected back in the Caledon Magistrate’s Court on later today.
Several sources with links to the South African “gang underworld” had said the drugs were discovered on a farm owned by Sea Point controversial businessman Mark Lifman. Lifman has however denied this.
It was a tip to the police from the farmworkers who uncovered the drugs.
The foreman at Witklip‚ near Villiersdorp, said workers raised the alarm when a pallet of wine destined for export to the Netherlands leaned precariously to one side.
“We unpacked the boxes. The first two layers were wine but at the third layer the boxes were much lighter‚” Godfrey Potberg [said].
“We cut it open and saw the powder‚ then we called the police.”
…Potberg said the pallets arrived at the farm in a van from Strand‚ and were due to be loaded into a container. Rodrigues had left the farm when the workers raised the alarm‚ he said‚ but he was called back by police.
“He was very nervous‚ he was smoking a lot of cigarettes. He wanted to make a phone call but the police took his phone away‚” he said.
Potberg said it was the first time workers at Witklip‚ which produces Eerste Hoop wines‚ had packed a consignment for someone else and it was the first time he had met Rodrigues or seen him on the farm. | <urn:uuid:379f2c5d-8704-481c-88aa-1042f213eb46> | CC-MAIN-2017-47 | http://bluemercury.co.za/breaking-news/south-africas-biggest-drug-bust/ | 2017-11-17T21:10:28Z | s3://commoncrawl/crawl-data/CC-MAIN-2017-47/segments/1510934803944.17/warc/CC-MAIN-20171117204606-20171117224606-00078.warc.gz | en | 0.989529 | 427 | [
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- The artwork for the Three Man Band half of the 7".
"Owsley (In Theory & Practice)," "Soul Sucker"
The dark specter of the '60s looms large on the Apache Dropout/Three Man Band split 7 inch record. Those with a hankering for psychedelic rock and garage rock will have their hunger well sated by both sides of the 7".
The effects used on the sustained piano that opens "Owsley (In Theory & Practice)" recall the playfulness of late '60s acts that discovered the joy of using the studio as another instrument. Along with the obvious sonic debt to psychedelia, the title of the cut is a nod to the infamous LSD magnate, Owsley Stanley. Stanley was a prolific manufacturer of acid, producing more than 1.25 million doses of LSD between 1965 and 1967.
"Owsley (In Theory & Practice)" calls to mind "Final Solution" by Cleveland-based proto-punks, Pere Ubu. Like "Final Solution," "Owsley (In Theory & Practice)" makes excellent use of tension and release to give the track a menacing aura; it's the sonic equivalent of an acid trip gone wrong. At times "Owsley (In Theory & Practice)" recalls The Electric Prunes, Amboy Dukes and other '60s bands plucked from obscurity on to one of Rhino's tastefully curated Nuggets box sets.
- Artwork for the Apache Dropout's "Soul Sucker."
Like the flip side, "Soul Sucker" carries the 60s sound, but is decidedly more bluesy and carries some of the feedback-drenched guitar freak-outs of The Velvet Underground. "Soul Sucker" is very much in line with the trend of '60s bands playing rock and roll with a more aggressive bent and greater guitar distortion.
It's a trend one can see in The Yardbirds, particularly when Jeff Beck was a member of the group. There are times where the track's rock and roll roots peek through, like a guitar solo that sounds right out of the Chuck Berry playbook. Singer Sonny Blood recalls the vocals of Lux Interior, particularly in Blood's enunciation of lyrics. His slurred syllables give the track a slightly psych tinge, though the track is more akin to The Seeds or The Sonics than the Standells.
A major problem for acts with a retro sound is blending their influences into something new, but Three Man Band and Apache Dropout are well on their way. They have a strong grasp of stylistic tropes of their influences and a knack for writing music, that while perhaps not the most innovative, is well-crafted and worthy of a least a few spins. | <urn:uuid:499d45d7-16db-4f42-b706-65222c2574c2> | CC-MAIN-2017-47 | http://m.nuvo.net/indianapolis/review-apache-dropout-three-man-band/Content?oid=2463893 | 2017-11-17T21:15:08Z | s3://commoncrawl/crawl-data/CC-MAIN-2017-47/segments/1510934803944.17/warc/CC-MAIN-20171117204606-20171117224606-00078.warc.gz | en | 0.949466 | 557 | [
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"Renegade", the new video from HOLLYWOOD UNDEAD, can be seen below. The song is taken from the band's fifth full-length album, "V" (Five), which will be released on October 27.HOLLYWOOD UNDEAD's Johnny 3 Tears stated about the track: "I hope this song and video can inspire kids to believe that they can change the world, and it's up to them if it's for the better or worse. The truth is that the kids of today are the renegades of tomorrow." Regarding "V", Johnny 3 Tears said: "We're five brothers, and this is our fifth record. Nothing gets to the essence of the music like this number does. Numerology has a lot of power. When we said 'Five', it just made sense. The fact that we could all agree on one word codifies who we are. It also nods back to 'No. 5' from our first album, because it was our fifth song. Moreover, it hints at this secret society of fans supporting us for the past decade. The number is significant, and this is a significant moment for us." HOLLYWOOD UNDEAD's new set of dates in support of "V" will begin on September 30 at the Louder Than Life festival. For all U.S. headline shows, BUTCHER BABIES will serve as direct support.
To comment on a BLABBERMOUTH.NET story or review, you must be logged in to an active personal account on Facebook. Once you're logged in, you will be able to comment. User comments or postings do not reflect the viewpoint of BLABBERMOUTH.NET and BLABBERMOUTH.NET does not endorse, or guarantee the accuracy of, any user comment. To report spam or any abusive, obscene, defamatory, racist, homophobic or threatening comments, or anything that may violate any applicable laws, use the "Report to Facebook" and "Mark as spam" links that appear next to the comments themselves. To do so, click the downward arrow on the top-right corner of the Facebook comment (the arrow is invisible until you roll over it) and select the appropriate action. You can also send an e-mail to blabbermouthinbox(@)gmail.com with pertinent details. BLABBERMOUTH.NET reserves the right to "hide" comments that may be considered offensive, illegal or inappropriate and to "ban" users that violate the site's Terms Of Service. Hidden comments will still appear to the user and to the user's Facebook friends. If a new comment is published from a "banned" user or contains a blacklisted word, this comment will automatically have limited visibility (the "banned" user's comments will only be visible to the user and the user's Facebook friends). | <urn:uuid:184eae80-fcbb-43b5-866d-11bfe1dac854> | CC-MAIN-2017-47 | http://www.blabbermouth.net/news/hollywood-undead-releases-video-for-renegade/ | 2017-11-17T21:24:56Z | s3://commoncrawl/crawl-data/CC-MAIN-2017-47/segments/1510934803944.17/warc/CC-MAIN-20171117204606-20171117224606-00078.warc.gz | en | 0.933748 | 592 | [
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For a fast, versatile ride that gets your where you want to go, climb aboard Haro’s Aire. Its lightweight aluminum step-through frame and durable steel fork are tuned to be quick and nimble while delivering all-day comfort. You’ll have all the gears you can handle with Shimano’s 21-speed drivetrain, keeping you rolling efficiently, both up and downhill, and a powerful pair of disc brakes give you the stopping power you need to safely and confidently navigate city streets, bike paths, and more. Haro carefully considered your comfort when they outfitted the Aire's cockpit with supportive, stylish components from Haro and Pivit. Who knew that getting around town could be this much fun?
|Charcoal/Orange / 15-inch||00691840188715||18865|
|Charcoal/Orange / 17-inch||00691840188722||18866|
|Limpet/White / 15-inch||00691840188739||18867|
|Limpet/White / 17-inch||00691840188746||18868| | <urn:uuid:3cf4b461-b2d6-4a58-8d05-5e571f5a00ac> | CC-MAIN-2017-47 | http://zanes.com/product/haro-aire-314758-1.htm | 2017-11-17T21:12:14Z | s3://commoncrawl/crawl-data/CC-MAIN-2017-47/segments/1510934803944.17/warc/CC-MAIN-20171117204606-20171117224606-00078.warc.gz | en | 0.853685 | 236 | [
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Prayers for the Dead Paperback
Dr Azor Sparks is a genius. A famous surgeon set to transform the chances for heart transplant patients.
He's also a pillar of the local church and a devoted family man.
So who would want to murder him? The answer, Lt. Peter Decker concludes, is many people. Sparks was obsessed by religion and his medical crusades.Yet he had odd hobbies, like riding with a pack of bikers.
His family, too, is not harmonious. The influence of Bram, the son who defied his Protestant father to become a Catholic priest, appears to be all that's preventing them from destroying each other. And, as beneficiaries of an astonishingly large trust fund, they all stand to gain from his death.
But then the evidence from a second murder points, unmistakably, to Bram, whom Decker thought was highly unlikely to be involved...
- Format: Paperback
- Pages: 448 pages
- Publisher: Headline Publishing Group
- Publication Date: 01/02/1997
- Category: Crime & mystery
- ISBN: 9780747252313
- EPUB from £3.49 | <urn:uuid:128169c4-f8c7-4a65-9c3a-26a512bb39e0> | CC-MAIN-2017-47 | https://www.hive.co.uk/Product/Faye-Kellerman/Prayers-for-the-Dead/1889095 | 2017-11-17T21:38:46Z | s3://commoncrawl/crawl-data/CC-MAIN-2017-47/segments/1510934803944.17/warc/CC-MAIN-20171117204606-20171117224606-00078.warc.gz | en | 0.965603 | 242 | [
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PURPOSE OF THE STUDY.
Many families are wary of giving artificial sweetener in high doses to their children. This objective of this study was to determine whether oral viscous budesonide (OVB) using Neocate Nutra, a hypoallergenic nutritional supplement, as a delivery vehicle is at least as efficacious as OVB mixed with Splenda at healing eosinophilic esophagitis (EoE).
Patients with EoE treated with OVB at the Boston Children’s Hospital Eosinophilic Gastrointestinal Disorder program between June 2008 and June 2013. Diagnosis was based on consensus recommendations.
This was a retrospective chart review. No dietary or medication changes were made between OVB initiation and follow-up endoscopy. The primary outcome measured was histologic response defined as change in peak eosinophil count to <15 eosinophils per high-power field (eos/HPF) after at least 10 weeks of therapy.
Forty-six children were treated with OVB mixed with Splenda (10 packets per milligram budesonide) and 14 with Neocate Nutra (2.5 cm3 per milligram budesonide). The 2 groups were not significantly different in their demographic or clinical characteristics. On follow-up endoscopy, 30 of 46 patients on Splenda and 13 of 14 patients on Neocate Nutra achieved histologic response. Mean pre- and posttreatment peak eosinophil counts for the Splenda group were 59.5 eos/HPF (range 20–180 ) and 25.5 eos/HPF (range 0–200 ), respectively. Mean pre- and posttreatment peak eosinophil counts for the Neocate Nutra group were 62 eos/HPF (range 20–120 ) and 9 eos/HPF (range 0–100 ), respectively. The odds ratio of success with Neocate Nutra as compared with Splenda was 6.93 (95% confidence interval 0.83–57.91, P = .0728), demonstrating noninferiority of Neocate Nutra.
OVB-Neocate Nutra is at least as effective as OVB-Splenda at treating children with EoE.
Although Splenda is generally considered to be safe, consumers are increasingly drawn to “natural” and “organic” products despite lack of clear evidence that the artificial sweetener has harmful effects in humans. Neocate Nutra, a medical food, is an amino acid–based powder prepared into a semisolid (puree consistency) for infants with feeding difficulties and food allergies. Use of Neocate Nutra as mixing agent to create a viscous budesonide slurry provides a potential alternative to the standard recipe with Splenda. Limitations include a small study sample and use of retrospective chart review, limiting assessment of medication compliance and nonuniform intervals between endoscopies.
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Published On : 17 May 2017
QYReseachReports.com has added a new research report in its repertoire. The report titled “Global Website Builders Market Size, Status and Forecast 2022”, explains all the factors that influence and control the global website builders market. It does this using recent history in the global website builders market till 2016. It proceeds to compile a precision-driven industry forecast which then becomes the cornerstone for a business in the global website builders market making its next strategic decision.
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The top companies in the global website builders market are elaborated on using multiple perspectives that are derived through SWOT analyses, a product specification and picture, and the company profile. This section also contains the revenue, gross, cost, price, production, and capacity in the global website builders market. The key players, as explained in the report, are Weebly, Squarespace, Wix, Dudaone, Yola, Jimdo, Yahoo, Godaddy, Homestead, Ibuilt, Dudamobile, Gomobi, Onbile, Mofuse, Qfuse, Activemobi, and Tappinn. Details provided on these leading companies in the global website builders market helps obtain a holistic picture in terms of current trends and upcoming predictions.
The report also contains a chapter on the members of the supply chain in the global website builders market. This involves the supply chain relationship management and the key consumers. The report on the global website builders market also includes a section dedicated to the analysis of marketing traders of website building in the world. This section contains the status of all marketing channels in the world today, ex-work price, end buyer price, and channel price analysis, and a list of the various distributors and service providers in the global website builders market.
Trending Reports : | <urn:uuid:c4ac6f26-47a7-42e1-8e29-177349787bf5> | CC-MAIN-2017-47 | https://www.qyresearchreports.com/pressrelease/website-builders-market.htm | 2017-11-18T16:00:21Z | s3://commoncrawl/crawl-data/CC-MAIN-2017-47/segments/1510934804976.22/warc/CC-MAIN-20171118151819-20171118171819-00078.warc.gz | en | 0.928191 | 660 | [
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While you can always contact me to set up a time to discuss your research, sometimes you just need a quick answer to point you in the right direction.
The Research Help tab on the library website offers a number of options for getting help with your questions:
This guide is designed as a starting point for your library research for SOC2130: Sociological Theory.
While there is a lot of useful information to be found online, these can contain questionable 'facts', bias and prejudices, or even outright lies. Therefore, it's best to begin your research with the Library, to make sure that the information you use in your analysis is from quality sources.
This guide will highlight certain resources that you should use in learning about your theorists and their research, and will provide an outline of the steps needed to collect the information necessary to complete your research paper.
Feel free to contact me with any questions you have, or to set up a time to meet one-on-one for help with your research. | <urn:uuid:f3d5fd83-1efc-496b-90ca-4c9ec0673823> | CC-MAIN-2017-47 | http://guides.wpunj.edu/soc2130 | 2017-11-19T10:10:32Z | s3://commoncrawl/crawl-data/CC-MAIN-2017-47/segments/1510934805541.30/warc/CC-MAIN-20171119095916-20171119115916-00078.warc.gz | en | 0.925653 | 204 | [
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If you don’t know who Pete Brown is, you should. Named the “Beer Writer of the Year” in 2009 by the British Guild of Beer Writers*, Brown writes from a deep well of beerly knowledge with his trademark effortless, engaging prose. Even if you have little experience with the traditional British beer and real ale that he predominantly writes about, his talents as an author and storyteller make him a must-read. He’s written three full-length books thus far with his best being the brilliant Hops and Glory in which Brown actually sails from Burton-on-Trent, England (home of Bass Ale) to India with a barrel of IPA in order to retrace the historical journey of the ubiquitous and much-loved beer style. Of course, if you’d prefer not to plunk down cash on a book, you can always follow his blog for free at petebrown.blogspot.com. But before you make his far superior blog your homepage instead of Aleheads.com, remember that Brown’s not an American and therefore he hates our freedom and our football and our Krispy Kreme milkshakes.
*Why don’t we have an American Guild of Beer Writers? Or guilds in general for that matter?
One of my favorite recent aspects of the Pete Brown Experience has been his development of a monthly series of video blogs with Peter Amor, founder of the Wye Valley Brewery in Herefordshire. I love everything about these videos. I love learning about breweries and beers I’ve never heard of in tiny corners of the UK. I love how Brown seems uncomfortable in front of the camera until he has a few pints in him and then turns into a seasoned pro. I love that he worries about repeating himself when doing the video tasting notes (don’t worry, Pete…there are only so many ways you can really describe hop-flavored, fermented sugar-water). And I LOVE that his cohort, Peter Amor (who funded this project out of his own pocket), might be the most British man on Earth. He’s curmudgeonly, fond of wearing bowties and silly hats, and rants endlessly about the damn government interfering with his damn drinking. After watching this series of videos, Peter Amor immediately rose to the top of my list of “people I’d like to have a session with at a pub”.
Earlier this month, Brown wrote an op-ed piece in the Publican’s Morning Advertiser (Note: I have no idea what this is…British magazines have silly names) decrying the lack of interest in the vlog series. There seemed to be zero support for the videos from any British periodicals or industry insiders. Brown and Amor were even met with indifference if not outright scorn by CAMRA (Britain’s “CAMpaign for Real Ale” which is loved and hated in equal measures by British Aleheads). This is a shame. The videos are well produced, well executed, and full of great tidbits about British beer and British beer culture.
I realize that Aleheads focuses almost exclusively on the American craft beer movement, but I won’t sit idly by while a resource like Brown and Amor’s vlogs go unwatched. I know our small corner of the blogosphere probably won’t crash Brown’s servers with traffic, but if even a few dozen of our loyal readers check out these wonderful videos, I’ll be happy. So if you have a few minutes over the next few days and weeks, click on this link, scroll through a year’s worth of excellent vlogs, and bask in Pete Brown’s beer knowledge and Peter Amor’s unparalleled Britishness. Cheerio! | <urn:uuid:603ef973-e14d-4f10-8df8-cc2cfec4a9c3> | CC-MAIN-2017-47 | https://aleheads.com/2011/08/30/bowties-and-silly-hats/ | 2017-11-19T10:10:08Z | s3://commoncrawl/crawl-data/CC-MAIN-2017-47/segments/1510934805541.30/warc/CC-MAIN-20171119095916-20171119115916-00078.warc.gz | en | 0.954987 | 796 | [
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On Monday, the National Review Online reported that MSNBC anchor Thomas Roberts bashed the children of contributor Mike Barnicle for being white, male, and presumably straight during a discussion on the Zimmerman acquittal on “Morning Joe.”
According to Roberts, Barnicle’s sons have “hit the American trifecta of privilege” in ways that Trayvon Martin and others — presumably blacks and Hispanics — have not.
“Barnicle,” Andrew Johnson wrote, “was describing how ‘truly unsettling’ he found it that he didn’t have to worry about warning his sons how to behave around law enforcement because they are white when Thomas Roberts interjected.”
“With all due respect, your three boys have hit the American trifecta of privilege,” Roberts said. “They are white, straight males, presumably.”
“If you are an ‘other’ in this country,” he added, ”and that means if you are an LGBT, if you are Hispanic, if you are black, if you are a woman, right now, we are fighting to prove why ‘other’ is not bad, and why we are due the value of American rights.”
“Trayvon’s rights were obviously violated,” he continued. ”Stalked, followed, presumed to be suspicious from the get-go.”
An all-female jury, however, found Zimmerman not guilty of any wrong-doing in the shooting death of Trayvon Martin.
Supporters of Trayvon Martin, upset with the verdict, have protested, blocked traffic on a California freeway and issued death threats against Zimmerman, his wife, attorney and the jurors in the case.
Pressure is now being put on the Justice Department to file civil rights charges against Zimmerman, even though the FBI found that racism played no role in the shooting.
Roberts, however, did not discuss the murder of Joshua Heath Chellow, a white man who was killed earlier this month when black gang members beat him and forced him into the path of an oncoming vehicle.
Apparently, in MSNBC’s view, white people should forget about equal protection under the law as well.
Video of the exchange can be seen here.
- Former DOJ attorney: Zimmerman prosecution sparked by New Black Panthers
- Video: Group of black men beat white man over Zimmerman verdict (Video)
- FBI report: No evidence George Zimmerman a racist
- Photos reveal Obama marched with Black Panthers in 2007
- Trayvon Martin protesters shut down L.A. freeway, police use rubber bullets
- Singer Toni Braxton on Zimmerman verdict: ‘I am embarrassed to be an American’
- New Black Panther leader: Kill white babies by bombing nurseries
- ‘Twitter lynch mob’ targets Zimmerman jury with death threats
- Atlanta Falcons’ Roddy White: Zimmerman jurors should ‘go home and kill themselves’ | <urn:uuid:5eaebcec-8590-48e9-b25f-ab3068baa19f> | CC-MAIN-2017-47 | https://libertyunyielding.com/2013/07/15/msnbcs-thomas-roberts-bashes-guests-children-for-being-white-male-presumably-straight/ | 2017-11-19T10:33:11Z | s3://commoncrawl/crawl-data/CC-MAIN-2017-47/segments/1510934805541.30/warc/CC-MAIN-20171119095916-20171119115916-00078.warc.gz | en | 0.941995 | 621 | [
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In a move to continue expanding services and diversifying its core telecommunications and technology services holdings, C Spire says it will acquire Hattiesburg’s MegaGate Broadband, a regional provider of IP voice, data, and business telecommunications solutions.
MegaGate serves most Mississippi business markets as a competitive local exchange telecommunications provider and as an Internet service provider. Founded in 1990, the company began operations in 1998 as a division of Cellular One, a wireless communications provider serving markets in south Mississippi.
C Spire did not provide the cost of the acquisition.
Kevin Pack, CEO of MegaGate, said he thinks MegateGate customers “will be in good hands as C Spire brings its customer-inspired approach to their business technology and communications needs.”
The acquisition, which requires regulatory approval, is the latest in a series of moves by the C Spire that the Ridgeland-based company says are part of an accelerated and expanded focus on “innovative business, government, and consumer technology solutions.”
Last September, C Spire launched the nation’s largest statewide initiative to bring 1 Gbps fiber-based Internet access to homes in nine Mississippi cities. In February, the company acquired Mobile, Ala.-based Callis Communications, a fast-growing provider of cloud-based communications services for businesses.
In one of its most aggressive efforts, C Spire plans to open in November a new $23 million Tier III+ data center in Starkville, which will power a full suite of enterprise cloud services and solutions for businesses across the Southeast region.
C Spire expects final regulatory approval of the merger within the next 60 days.
Started in 1998 as a division of Cellular One, MegaGate became a facilities-based competitive local exchange carrier and Internet service provider, initially offering dial-up Internet service to its wireless customers, and later adding voice, data, hosted phone service, and a variety of other telecommunications solutions to small and medium sized businesses throughout the state. | <urn:uuid:5872c4a1-e58f-460e-adf6-dd305f9ee2e0> | CC-MAIN-2017-47 | http://msbusiness.com/2014/06/c-spire-deal-acquire-regional-telecom-megagate/ | 2017-11-20T06:31:56Z | s3://commoncrawl/crawl-data/CC-MAIN-2017-47/segments/1510934805914.1/warc/CC-MAIN-20171120052322-20171120072322-00078.warc.gz | en | 0.946685 | 409 | [
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By Anne Midgette
Cultural diplomacy is a significant activity for symphony orchestras. The Boston Symphony Orchestra toured Russia in 1956. The Philadelphia Orchestra went to China in 1973. The New York Philharmonic played Pyongyang in 2008; the Minnesota Orchestra went to Cuba this past May. And on Friday night, the music of Dvorak’s “New World” symphony was heard in Tehran, performed from the original music the New York Philharmonic has guarded since the work’s 1893 premiere.
This orchestra, though, wasn’t American. It was the China Philharmonic.
“The New York Philharmonic gave me the original parts,” said China Philharmonic music director Long Yu, speaking by cellphone from an airport en route to Greece the day after a concert he described as historic. “So it’s very touching if you see the music, you’re touching that history.”
The China Philharmonic, created in 2000 from what had formerly been the China Broadcasting Symphony Orchestra, and still technically the state radio orchestra, is wrapping up a six-stop Silk Road tour with Long Yu. Unlike Yo-Yo Ma’s ongoing Silk Road Project, which since 1998 has celebrated the Silk Road’s melange of cultures and history of exchange through chamber music and educational programs, the China Philharmonic’s tour takes a traditional approach to cultural diplomacy. The orchestra is playing Chinese and Western repertory and effectively showcasing its strengths to China’s not-so-distant geographical neighbors.
It also showcases Long Yu, a superpower of China’s burgeoning music world who also leads the Guangzhou Symphony Orchestra, the Shanghai Symphony Orchestra, the Beijing Music Festival, and the Shanghai Orchestra Academy, in a role he would dearly like to assume: that of cultural diplomat.
Speaking the day after the concert, which was met with the requisite standing ovation, two encores and seven curtain calls (not an unprecedented number on international tours), he embraced the time-honored rhetoric trotted out on such occasions of “the universal language of music” and the joys of bringing the treasures of the West to a new audience.
“You can see how the people are looking for life, and the passion for life,” he said, waxing eloquent on the beauties of Tehran.
The West tends to think of China as a recipient of its cultural diplomacy, not as its purveyor. And yet at a time when some Iranians are chanting “Death to America” in the streets, it’s a Chinese orchestra, rather than an American one, that brought this American-flavored music, with the imprimatur of its American parts and what Long Yu describes as “liberal ideas,” to Iran.
The Pittsburgh Symphony, which last played in Tehran in 1964 as part of a tour sponsored by the State Department, voiced hopes last year of playing there again; and it’s been rumored that Daniel Barenboim may lead the Berlin Staatskapelle there during Angela Merkel’s state visit in October. But China has beaten them to the punch — with a work that symbolizes the appropriation of traditional forms by a “new world.”
On Friday, there were a couple of “new worlds” at play. China is planting a flag to show itself as a player in the international cultural community. But Tehran was also spreading its wings as a city that wants such culture. The performance, in fact, was shared between the China Philharmonic and the Tehran Symphony Orchestra, founded in 1933, defunct for several years, and revived this past April with what by one account was a struggling but eager performance of Beethoven’s Ninth.
On Friday, led by Ali Rahbari (who has had a distinguished career in the West, and has come under fire in Iran in the past for “promoting Western values”), the ensemble played Rimsky-Korsakov’s “Scheherazade” — a snapshot of the East through Western eyes. | <urn:uuid:39eb91e7-9059-4d9f-b75e-f9b92d1879f0> | CC-MAIN-2017-47 | https://www.8vamusicconsultancy.com/news/2016/6/26/china-philharmonics-silk-road-tour-wends-to-iran | 2017-11-20T05:55:37Z | s3://commoncrawl/crawl-data/CC-MAIN-2017-47/segments/1510934805914.1/warc/CC-MAIN-20171120052322-20171120072322-00078.warc.gz | en | 0.959261 | 857 | [
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The Camera also notes that the defense is asking that investigative committee member Jose Limon be allowed to testify out of order. If that's okayed, it wouldn't be surprising if Churchill's testimony is pushed back another day.
For the final question CU attorney Patrick O'Rourke asked indigenous studies professor Michael Yellow Bird during his re-cross Wednesday morning, he pulled up [sic] a transcript of previous testimony the professor had given to CU's Privilege & Tenure Committee and asked him if he had made the statement that "fabricated, made-up accounts promote the truth."
With a slight pause, Yellow Bird said yes.
Update: Vince Carroll: "Churchill's lawyer says anything goes." | <urn:uuid:06229bbd-f2bf-4abe-a475-19f9f3b12f2e> | CC-MAIN-2017-47 | http://thedrunkablog.blogspot.com/2009/03/more-hole-digging-from-michael-yellow.html | 2017-11-21T00:44:42Z | s3://commoncrawl/crawl-data/CC-MAIN-2017-47/segments/1510934806309.83/warc/CC-MAIN-20171121002016-20171121022016-00078.warc.gz | en | 0.968904 | 145 | [
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The original vision of Sweet Gale Gardens was to include ecological landscape design services. For the 2017 season, we are reincorporating it into our business for a small, select group of clients. We are looking to partner with people and organizations who:
Believe in supporting pollinators and wildlife
Draw inspiration from wild landscapes
Champion organic garden care
Swoon over colorful, scented, and unusual plants
Jessica holds a Master in Landscape Architecture from State University of New York College of Environmental Science and Forestry. Since graduating, she's honed her growing skills on the farm and diversified her knowledge of native plant species, spending most of her free time rambling in the woods, meadows, and wetlands.
We already have a small set of clients for 2017, would you like to be one of them?
Email at hello [@] sweetgalegardens.com | <urn:uuid:c41c35df-e565-447a-8ea7-40119e353366> | CC-MAIN-2017-47 | http://sweetgalegardens.com/gardens | 2017-11-21T19:07:58Z | s3://commoncrawl/crawl-data/CC-MAIN-2017-47/segments/1510934806422.29/warc/CC-MAIN-20171121185236-20171121205236-00078.warc.gz | en | 0.946818 | 181 | [
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RELEASED 20 years ago, The Holy Bible was the album where the Manic Street Preachers got serious and were taken seriously – a caustic, candid and claustrophobic classic which took on greater resonance when guitarist and chief lyricist Richey Edwards went missing a few months after its release.
Manic Street Preachers
Over the years, its cathartic depiction of self-harm and suicide might just have saved a few lives but it remains a dense proposition and it’s probably with good reason that the band have not played some of the material live since it was released.
For this anniversary tour, the remaining three-piece have chosen to recreate the confrontational look of the time, with the stage draped in camouflage netting and stocky frontman James Dean Bradfield unsettling in a sailor suit, and they stuck close to the sound if not quite the spirit of the music.
The original track listing was dispensed in order, complete with the album’s plethora of evocative vocal samples, but their performance never quite achieved lift-off despite some meaty, knotted post-punk guitar work from Bradfield.
Although The Holy Bible is not exactly endowed with anthems, the crowd seized on the more singalongable refrains from Of Walking Abortion and Die In The Summertime. Join in if you know the lyrics... “Happy f***ing Christmas,” noted bassist Nicky Wire wryly, before (relatively) light relief arrived in a second half bookended by the mighty Motorcycle Emptiness and Design For Life.
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CHICAGO , IL 60608
4 Beds, 3.5 Bath(s) $5,500 per month
Property Type: Residential Rental
Heart of Chicago
|Remarks - $5500/MTH. UNFURNISHED OR $5800/MTH FURNISHED! $5500 SEC. DEP. AND $2500 MOVE IN FEE PILSEN! UIC! IMD! THIS HUGE NEW CONSTRUCTION 4 BEDROOM / 3.5 BATHROOM HOME WITH 2.5 CAR GARAGE IN PILSEN! HIGH END FINISHES! TALL 2 CAR GARAGE, TALL! 2 LAUNDRY ROOMS! CENTRAL HEAT AND A/C! KITCHEN WITH STAINLESS STEEL APPLIANCES AND GRANITE COUNTERTOPS! DISHWASHER AND MICROWAVE! 2 IN UNIT LAUNDRY AREAS! QUIET NEIGHBORHOOD, SEPARATE KITCHEN AND BREAKFAST NOOK! HARDWOOD, CARPET AND TILE FLOORING! BEAUTIFUL FRONT PATIO! HIGH END POOL TABLE AND GAME TABLE! SURROUND SOUND THROUGH HOUSE AND SECURITY CAMERA SYSTEM! CLOSE TO BUS AND TRAIN LINE AND -55 AND 290 EXPRESSWAYS! JACUZZI AND STEAM SHOWER! HUGE ROOFTOP DECK AND GARAGE ROOFTOP DECK! MASTER BEDROOM WITH BALCONY! TALL CEILINGS! CLOSE TO HARRISON PARK! CLOSE TO UIC AND MEDICAL DISTRICT! CLOSE TO RUSH HOSPITAL!! NEAR HARRISON PARK THAT HAS BASEBALL FIELDS, BASKETBALL COURTS, TENNIS COURT, INDOOR POOL! PETS OK! MUST HAVE EXCELLENT CREDIT!
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Information deemed reliable but not guaranteed. | <urn:uuid:818d9090-d1ac-4047-9105-0a4847370df4> | CC-MAIN-2017-47 | http://www.livebytransit.com/listings/8249669 | 2017-11-22T14:59:35Z | s3://commoncrawl/crawl-data/CC-MAIN-2017-47/segments/1510934806609.33/warc/CC-MAIN-20171122141600-20171122161600-00078.warc.gz | en | 0.69417 | 554 | [
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Discussion in 'Classifieds' started by Pat Kiernan, Mar 29, 2013.
On the lookout if anyone has one or something close? thanks pat
I have a 4wt 10ft RPL.
Willing to part with her.
Make an offer.
Pm sent big wally pat
Still looking if anyone has one they arent using thanks pat by the way does anyone know was the vps or vps light made in this configuration?
take a look at an echo ion 10 footer. i picked up 2 on this site for $100 EACH. THEY ARE JUST ABOUT AS GOOD AS A SAGE. i use them for steelhead. lots cheaper. mike w
Hi I dont the there is an ion in that configuration but believe they are lovely rods so still looking pat | <urn:uuid:8d6e485e-379d-4123-ac61-7530c5ce8fde> | CC-MAIN-2017-47 | http://www.washingtonflyfishing.com/forum/threads/sage-rpl-10-foot-4-or-5-wt.87910/ | 2017-11-22T14:52:15Z | s3://commoncrawl/crawl-data/CC-MAIN-2017-47/segments/1510934806609.33/warc/CC-MAIN-20171122141600-20171122161600-00078.warc.gz | en | 0.941809 | 167 | [
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The split hand phenomenon refers to predominant wasting of thenar muscles and is an early and specific feature of amyotrophic lateral sclerosis (ALS). A novel split hand index (SI) was developed to quantify the split hand phenomenon, and its diagnostic utility was assessed in ALS patients. The split hand index was derived by dividing the product of the compound muscle action potential (CMAP) amplitude recorded over the abductor pollicis brevis and first dorsal interosseous muscles by the CMAP amplitude recorded over the abductor digiti minimi muscle. In order to assess the diagnostic utility of the split hand index, ALS patients were prospectively assessed and their results were compared to neuromuscular disorder patients. The split hand index was significantly reduced in ALS when compared to neuromuscular disorder patients (P<0.0001). Limb-onset ALS patients exhibited the greatest reduction in the split hand index, and a value of 5.2 or less reliably differentiated ALS from other neuromuscular disorders. Consequently, the split hand index appears to be a novel diagnostic biomarker for ALS, perhaps facilitating an earlier diagnosis.
27 Related JoVE Articles!
Annotation of Plant Gene Function via Combined Genomics, Metabolomics and Informatics
Given the ever expanding number of model plant species for which complete genome sequences are available and the abundance of bio-resources such as knockout mutants, wild accessions and advanced breeding populations, there is a rising burden for gene functional annotation. In this protocol, annotation of plant gene function using combined co-expression gene analysis, metabolomics and informatics is provided (Figure 1
). This approach is based on the theory of using target genes of known function to allow the identification of non-annotated genes likely to be involved in a certain metabolic process, with the identification of target compounds via metabolomics. Strategies are put forward for applying this information on populations generated by both forward and reverse genetics approaches in spite of none of these are effortless. By corollary this approach can also be used as an approach to characterise unknown peaks representing new or specific secondary metabolites in the limited tissues, plant species or stress treatment, which is currently the important trial to understanding plant metabolism.
Plant Biology, Issue 64, Genetics, Bioinformatics, Metabolomics, Plant metabolism, Transcriptome analysis, Functional annotation, Computational biology, Plant biology, Theoretical biology, Spectroscopy and structural analysis
Changing the Direction and Orientation of Electric Field During Electric Pulses Application Improves Plasmid Gene Transfer in vitro
Institutions: University of Ljubljana, University of Ljubljana.
Gene electrotransfer is a physical method used to deliver genes into the cells by application of short and intense electric pulses, which cause destabilization of cell membrane, making it permeable to small molecules and allows transfer of large molecules such as DNA. It represents an alternative to viral vectors, due to its safety, efficacy and ease of application. For gene electrotransfer different electric pulse protocols are used in order to achieve maximum gene transfection, one of them is changing the electric field direction and orientation during the pulse delivery. Changing electric field direction and orientation increase the membrane area competent for DNA entry into the cell. In this video, we demonstrate the difference in gene electrotransfer efficacy when all pulses are delivered in the same direction and when pulses are delivered by changing alternatively the electric field direction and orientation. For this purpose tip with integrated electrodes and high-voltage prototype generator, which allows changing of electric field in different directions during electric pulse application, were used. Gene electrotransfer efficacy is determined 24h after pulse application as the number of cells expressing green fluorescent protein divided with the number of all cells. The results show that gene transfection is increased when the electric field orientation during electric pulse delivery is changed.
Medicine, Issue 55, gene electrotransfer, GFP, changing the orientation of electric field, plasmid, gene, transfection
Polycrystalline Silicon Thin-film Solar cells with Plasmonic-enhanced Light-trapping
Institutions: University of New South Wales .
One of major approaches to cheaper solar cells is reducing the amount of semiconductor material used for their fabrication and making cells thinner. To compensate for lower light absorption such physically thin devices have to incorporate light-trapping which increases their optical thickness. Light scattering by textured surfaces is a common technique but it cannot be universally applied to all solar cell technologies. Some cells, for example those made of evaporated silicon, are planar as produced and they require an alternative light-trapping means suitable for planar devices. Metal nanoparticles formed on planar silicon cell surface and capable of light scattering due to surface plasmon resonance is an effective approach.
The paper presents a fabrication procedure of evaporated polycrystalline silicon solar cells with plasmonic light-trapping and demonstrates how the cell quantum efficiency improves due to presence of metal nanoparticles.
To fabricate the cells a film consisting of alternative boron and phosphorous doped silicon layers is deposited on glass substrate by electron beam evaporation. An Initially amorphous film is crystallised and electronic defects are mitigated by annealing and hydrogen passivation. Metal grid contacts are applied to the layers of opposite polarity to extract electricity generated by the cell. Typically, such a ~2 μm thick cell has a short-circuit current density (Jsc
) of 14-16 mA/cm2
, which can be increased up to 17-18 mA/cm2
(~25% higher) after application of a simple diffuse back reflector made of a white paint.
To implement plasmonic light-trapping a silver nanoparticle array is formed on the metallised cell silicon surface. A precursor silver film is deposited on the cell by thermal evaporation and annealed at 23°C to form silver nanoparticles. Nanoparticle size and coverage, which affect plasmonic light-scattering, can be tuned for enhanced cell performance by varying the precursor film thickness and its annealing conditions. An optimised nanoparticle array alone results in cell Jsc
enhancement of about 28%, similar to the effect of the diffuse reflector. The photocurrent can be further increased by coating the nanoparticles by a low refractive index dielectric, like MgF2
, and applying the diffused reflector. The complete plasmonic cell structure comprises the polycrystalline silicon film, a silver nanoparticle array, a layer of MgF2
, and a diffuse reflector. The Jsc
for such cell is 21-23 mA/cm2
, up to 45% higher than Jsc
of the original cell without light-trapping or ~25% higher than Jsc
for the cell with the diffuse reflector only.
Light-trapping in silicon solar cells is commonly achieved via light scattering at textured interfaces. Scattered light travels through a cell at oblique angles for a longer distance and when such angles exceed the critical angle at the cell interfaces the light is permanently trapped in the cell by total internal reflection (Animation 1: Light-trapping)
. Although this scheme works well for most solar cells, there are developing technologies where ultra-thin Si layers are produced planar (e.g. layer-transfer technologies and epitaxial c-Si layers) 1
and or when such layers are not compatible with textures substrates (e.g. evaporated silicon) 2
. For such originally planar Si layer alternative light trapping approaches, such as diffuse white paint reflector 3
, silicon plasma texturing 4
or high refractive index nanoparticle reflector 5
have been suggested.
Metal nanoparticles can effectively scatter incident light into a higher refractive index material, like silicon, due to the surface plasmon resonance effect 6
. They also can be easily formed on the planar silicon cell surface thus offering a light-trapping approach alternative to texturing. For a nanoparticle located at the air-silicon interface the scattered light fraction coupled into silicon exceeds 95% and a large faction of that light is scattered at angles above critical providing nearly ideal light-trapping condition (Animation 2: Plasmons on NP)
. The resonance can be tuned to the wavelength region, which is most important for a particular cell material and design, by varying the nanoparticle average size, surface coverage and local dielectric environment 6,7
. Theoretical design principles of plasmonic nanoparticle solar cells have been suggested 8
. In practice, Ag nanoparticle array is an ideal light-trapping partner for poly-Si thin-film solar cells because most of these design principle are naturally met. The simplest way of forming nanoparticles by thermal annealing of a thin precursor Ag film results in a random array with a relatively wide size and shape distribution, which is particularly suitable for light-trapping because such an array has a wide resonance peak, covering the wavelength range of 700-900 nm, important for poly-Si solar cell performance. The nanoparticle array can only be located on the rear poly-Si cell surface thus avoiding destructive interference between incident and scattered light which occurs for front-located nanoparticles 9
. Moreover, poly-Si thin-film cells do not requires a passivating layer and the flat base-shaped nanoparticles (that naturally result from thermal annealing of a metal film) can be directly placed on silicon further increases plasmonic scattering efficiency due to surface plasmon-polariton resonance 10
The cell with the plasmonic nanoparticle array as described above can have a photocurrent about 28% higher than the original cell. However, the array still transmits a significant amount of light which escapes through the rear of the cell and does not contribute into the current. This loss can be mitigated by adding a rear reflector to allow catching transmitted light and re-directing it back to the cell. Providing sufficient distance between the reflector and the nanoparticles (a few hundred nanometers) the reflected light will then experience one more plasmonic scattering event while passing through the nanoparticle array on re-entering the cell and the reflector itself can be made diffuse - both effects further facilitating light scattering and hence light-trapping. Importantly, the Ag nanoparticles have to be encapsulated with an inert and low refractive index dielectric, like MgF2
, from the rear reflector to avoid mechanical and chemical damage 7
. Low refractive index for this cladding layer is required to maintain a high coupling fraction into silicon and larger scattering angles, which are ensured by the high optical contrast between the media on both sides of the nanoparticle, silicon and dielectric 6
. The photocurrent of the plasmonic cell with the diffuse rear reflector can be up to 45% higher than the current of the original cell or up to 25% higher than the current of an equivalent cell with the diffuse reflector only.
Physics, Issue 65, Materials Science, Photovoltaics, Silicon thin-film solar cells, light-trapping, metal nanoparticles, surface plasmons
Analysis of Fatty Acid Content and Composition in Microalgae
Institutions: Wageningen University and Research Center, Wageningen University and Research Center, Wageningen University and Research Center.
A method to determine the content and composition of total fatty acids present in microalgae is described. Fatty acids are a major constituent of microalgal biomass. These fatty acids can be present in different acyl-lipid classes. Especially the fatty acids present in triacylglycerol (TAG) are of commercial interest, because they can be used for production of transportation fuels, bulk chemicals, nutraceuticals (ω-3 fatty acids), and food commodities. To develop commercial applications, reliable analytical methods for quantification of fatty acid content and composition are needed. Microalgae are single cells surrounded by a rigid cell wall. A fatty acid analysis method should provide sufficient cell disruption to liberate all acyl lipids and the extraction procedure used should be able to extract all acyl lipid classes.
With the method presented here all fatty acids present in microalgae can be accurately and reproducibly identified and quantified using small amounts of sample (5 mg) independent of their chain length, degree of unsaturation, or the lipid class they are part of.
This method does not provide information about the relative abundance of different lipid classes, but can be extended to separate lipid classes from each other.
The method is based on a sequence of mechanical cell disruption, solvent based lipid extraction, transesterification of fatty acids to fatty acid methyl esters (FAMEs), and quantification and identification of FAMEs using gas chromatography (GC-FID). A TAG internal standard (tripentadecanoin) is added prior to the analytical procedure to correct for losses during extraction and incomplete transesterification.
Environmental Sciences, Issue 80, chemical analysis techniques, Microalgae, fatty acid, triacylglycerol, lipid, gas chromatography, cell disruption
Preparation of Silica Nanoparticles Through Microwave-assisted Acid-catalysis
Institutions: Oak Ridge Institute for Science and Education, Airbase Technology Division, Clemson University.
Microwave-assisted synthetic techniques were used to quickly and reproducibly produce silica nanoparticle sols
using an acid catalyst with nanoparticle diameters ranging from 30-250 nm by varying the reaction conditions. Through the selection of a microwave compatible solvent, silicic acid precursor, catalyst, and microwave irradiation time, these microwave-assisted methods were capable of overcoming the previously reported shortcomings associated with synthesis of silica nanoparticles using microwave reactors. The siloxane precursor was hydrolyzed using the acid catalyst, HCl. Acetone, a low-tan δ
solvent, mediates the condensation reactions and has minimal interaction with the electromagnetic field. Condensation reactions begin when the silicic acid precursor couples with the microwave radiation, leading to silica nanoparticle sol
formation. The silica nanoparticles were characterized by dynamic light scattering data and scanning electron microscopy, which show the materials' morphology and size to be dependent on the reaction conditions. Microwave-assisted reactions produce silica nanoparticles with roughened textured surfaces that are atypical for silica sols
produced by Stöber's methods, which have smooth surfaces.
Chemistry, Issue 82, Chemistry, chemical manufacturing, chemistry (general), materials (general), nanocomposites, catalysts (chemical), chemistry of compounds, Chemistry and Materials (General), Composite Materials, Inorganic, Organic and Physical Chemistry, Engineering (General), Microwave, nanoparticle, silica, silicic acid, NP, SiO2, synthesis
Glutamine Flux Imaging Using Genetically Encoded Sensors
Institutions: Virginia Tech.
Genetically encoded sensors allow real-time monitoring of biological molecules at a subcellular resolution. A tremendous variety of such sensors for biological molecules became available in the past 15 years, some of which became indispensable tools that are used routinely in many laboratories.
One of the exciting applications of genetically encoded sensors is the use of these sensors in investigating cellular transport processes. Properties of transporters such as kinetics and substrate specificities can be investigated at a cellular level, providing possibilities for cell-type specific analyses of transport activities. In this article, we will demonstrate how transporter dynamics can be observed using genetically encoded glutamine sensor as an example. Experimental design, technical details of the experimental settings, and considerations for post-experimental analyses will be discussed.
Bioengineering, Issue 89, glutamine sensors, FRET, metabolites, in vivo imaging, cellular transport, genetically encoded sensors
Microarray-based Identification of Individual HERV Loci Expression: Application to Biomarker Discovery in Prostate Cancer
Institutions: Joint Unit Hospices de Lyon-bioMérieux, BioMérieux, Hospices Civils de Lyon, Lyon 1 University, BioMérieux, Hospices Civils de Lyon, Hospices Civils de Lyon.
The prostate-specific antigen (PSA) is the main diagnostic biomarker for prostate cancer in clinical use, but it lacks specificity and sensitivity, particularly in low dosage values1
. ‘How to use PSA' remains a current issue, either for diagnosis as a gray zone corresponding to a concentration in serum of 2.5-10 ng/ml which does not allow a clear differentiation to be made between cancer and noncancer2
or for patient follow-up as analysis of post-operative PSA kinetic parameters can pose considerable challenges for their practical application3,4
. Alternatively, noncoding RNAs (ncRNAs) are emerging as key molecules in human cancer, with the potential to serve as novel markers of disease, e.g.
PCA3 in prostate cancer5,6
and to reveal uncharacterized aspects of tumor biology. Moreover, data from the ENCODE project published in 2012 showed that different RNA types cover about 62% of the genome. It also appears that the amount of transcriptional regulatory motifs is at least 4.5x higher than the one corresponding to protein-coding exons. Thus, long terminal repeats (LTRs) of human endogenous retroviruses (HERVs) constitute a wide range of putative/candidate transcriptional regulatory sequences, as it is their primary function in infectious retroviruses. HERVs, which are spread throughout the human genome, originate from ancestral and independent infections within the germ line, followed by copy-paste propagation processes and leading to multicopy families occupying 8% of the human genome (note that exons span 2% of our genome). Some HERV loci still express proteins that have been associated with several pathologies including cancer7-10
. We have designed a high-density microarray, in Affymetrix format, aiming to optimally characterize individual HERV loci expression, in order to better understand whether they can be active, if they drive ncRNA transcription or modulate coding gene expression. This tool has been applied in the prostate cancer field (Figure 1
Medicine, Issue 81, Cancer Biology, Genetics, Molecular Biology, Prostate, Retroviridae, Biomarkers, Pharmacological, Tumor Markers, Biological, Prostatectomy, Microarray Analysis, Gene Expression, Diagnosis, Human Endogenous Retroviruses, HERV, microarray, Transcriptome, prostate cancer, Affymetrix
The Use of Chemostats in Microbial Systems Biology
Institutions: New York University .
Cells regulate their rate of growth in response to signals from the external world. As the cell grows, diverse cellular processes must be coordinated including macromolecular synthesis, metabolism and ultimately, commitment to the cell division cycle. The chemostat, a method of experimentally controlling cell growth rate, provides a powerful means of systematically studying how growth rate impacts cellular processes - including gene expression and metabolism - and the regulatory networks that control the rate of cell growth. When maintained for hundreds of generations chemostats can be used to study adaptive evolution of microbes in environmental conditions that limit cell growth. We describe the principle of chemostat cultures, demonstrate their operation and provide examples of their various applications. Following a period of disuse after their introduction in the middle of the twentieth century, the convergence of genome-scale methodologies with a renewed interest in the regulation of cell growth and the molecular basis of adaptive evolution is stimulating a renaissance in the use of chemostats in biological research.
Environmental Sciences, Issue 80, Saccharomyces cerevisiae, Molecular Biology, Computational Biology, Systems Biology, Cell Biology, Genetics, Environmental Microbiology, Biochemistry, Chemostat, growth-rate, steady state, nutrient limitation, adaptive evolution
An Experimental and Bioinformatics Protocol for RNA-seq Analyses of Photoperiodic Diapause in the Asian Tiger Mosquito, Aedes albopictus
Institutions: Georgetown University, The Ohio State University.
Photoperiodic diapause is an important adaptation that allows individuals to escape harsh seasonal environments via a series of physiological changes, most notably developmental arrest and reduced metabolism. Global gene expression profiling via RNA-Seq can provide important insights into the transcriptional mechanisms of photoperiodic diapause. The Asian tiger mosquito, Aedes albopictus
, is an outstanding organism for studying the transcriptional bases of diapause due to its ease of rearing, easily induced diapause, and the genomic resources available. This manuscript presents a general experimental workflow for identifying diapause-induced transcriptional differences in A. albopictus.
Rearing techniques, conditions necessary to induce diapause and non-diapause development, methods to estimate percent diapause in a population, and RNA extraction and integrity assessment for mosquitoes are documented. A workflow to process RNA-Seq data from Illumina sequencers culminates in a list of differentially expressed genes. The representative results demonstrate that this protocol can be used to effectively identify genes differentially regulated at the transcriptional level in A. albopictus
due to photoperiodic differences. With modest adjustments, this workflow can be readily adapted to study the transcriptional bases of diapause or other important life history traits in other mosquitoes.
Genetics, Issue 93, Aedes albopictus Asian tiger mosquito, photoperiodic diapause, RNA-Seq de novo transcriptome assembly, mosquito husbandry
Identification of Key Factors Regulating Self-renewal and Differentiation in EML Hematopoietic Precursor Cells by RNA-sequencing Analysis
Institutions: The University of Texas Graduate School of Biomedical Sciences at Houston.
Hematopoietic stem cells (HSCs) are used clinically for transplantation treatment to rebuild a patient's hematopoietic system in many diseases such as leukemia and lymphoma. Elucidating the mechanisms controlling HSCs self-renewal and differentiation is important for application of HSCs for research and clinical uses. However, it is not possible to obtain large quantity of HSCs due to their inability to proliferate in vitro
. To overcome this hurdle, we used a mouse bone marrow derived cell line, the EML (Erythroid, Myeloid, and Lymphocytic) cell line, as a model system for this study.
RNA-sequencing (RNA-Seq) has been increasingly used to replace microarray for gene expression studies. We report here a detailed method of using RNA-Seq technology to investigate the potential key factors in regulation of EML cell self-renewal and differentiation. The protocol provided in this paper is divided into three parts. The first part explains how to culture EML cells and separate Lin-CD34+ and Lin-CD34- cells. The second part of the protocol offers detailed procedures for total RNA preparation and the subsequent library construction for high-throughput sequencing. The last part describes the method for RNA-Seq data analysis and explains how to use the data to identify differentially expressed transcription factors between Lin-CD34+ and Lin-CD34- cells. The most significantly differentially expressed transcription factors were identified to be the potential key regulators controlling EML cell self-renewal and differentiation. In the discussion section of this paper, we highlight the key steps for successful performance of this experiment.
In summary, this paper offers a method of using RNA-Seq technology to identify potential regulators of self-renewal and differentiation in EML cells. The key factors identified are subjected to downstream functional analysis in vitro
and in vivo
Genetics, Issue 93, EML Cells, Self-renewal, Differentiation, Hematopoietic precursor cell, RNA-Sequencing, Data analysis
Metabolic Labeling of Newly Transcribed RNA for High Resolution Gene Expression Profiling of RNA Synthesis, Processing and Decay in Cell Culture
Institutions: Max von Pettenkofer Institute, University of Cambridge, Ludwig-Maximilians-University Munich.
The development of whole-transcriptome microarrays and next-generation sequencing has revolutionized our understanding of the complexity of cellular gene expression. Along with a better understanding of the involved molecular mechanisms, precise measurements of the underlying kinetics have become increasingly important. Here, these powerful methodologies face major limitations due to intrinsic properties of the template samples they study, i.e.
total cellular RNA. In many cases changes in total cellular RNA occur either too slowly or too quickly to represent the underlying molecular events and their kinetics with sufficient resolution. In addition, the contribution of alterations in RNA synthesis, processing, and decay are not readily differentiated.
We recently developed high-resolution gene expression profiling to overcome these limitations. Our approach is based on metabolic labeling of newly transcribed RNA with 4-thiouridine (thus also referred to as 4sU-tagging) followed by rigorous purification of newly transcribed RNA using thiol-specific biotinylation and streptavidin-coated magnetic beads. It is applicable to a broad range of organisms including vertebrates, Drosophila
, and yeast. We successfully applied 4sU-tagging to study real-time kinetics of transcription factor activities, provide precise measurements of RNA half-lives, and obtain novel insights into the kinetics of RNA processing. Finally, computational modeling can be employed to generate an integrated, comprehensive analysis of the underlying molecular mechanisms.
Genetics, Issue 78, Cellular Biology, Molecular Biology, Microbiology, Biochemistry, Eukaryota, Investigative Techniques, Biological Phenomena, Gene expression profiling, RNA synthesis, RNA processing, RNA decay, 4-thiouridine, 4sU-tagging, microarray analysis, RNA-seq, RNA, DNA, PCR, sequencing
Establishment of Microbial Eukaryotic Enrichment Cultures from a Chemically Stratified Antarctic Lake and Assessment of Carbon Fixation Potential
Institutions: Miami University .
Lake Bonney is one of numerous permanently ice-covered lakes located in the McMurdo Dry Valleys, Antarctica. The perennial ice cover maintains a chemically stratified water column and unlike other inland bodies of water, largely prevents external input of carbon and nutrients from streams. Biota are exposed to numerous environmental stresses, including year-round severe nutrient deficiency, low temperatures, extreme shade, hypersalinity, and 24-hour darkness during the winter 1
. These extreme environmental conditions limit the biota in Lake Bonney almost exclusively to microorganisms 2
Single-celled microbial eukaryotes (called "protists") are important players in global biogeochemical cycling 3
and play important ecological roles in the cycling of carbon in the dry valley lakes, occupying both primary and tertiary roles in the aquatic food web. In the dry valley aquatic food web, protists that fix inorganic carbon (autotrophy) are the major producers of organic carbon for organotrophic organisms 4, 2
. Phagotrophic or heterotrophic protists capable of ingesting bacteria and smaller protists act as the top predators in the food web 5
. Last, an unknown proportion of the protist population is capable of combined mixotrophic metabolism 6, 7
. Mixotrophy in protists involves the ability to combine photosynthetic capability with phagotrophic ingestion of prey microorganisms. This form of mixotrophy differs from mixotrophic metabolism in bacterial species, which generally involves uptake dissolved carbon molecules. There are currently very few protist isolates from permanently ice-capped polar lakes, and studies of protist diversity and ecology in this extreme environment have been limited 8, 4, 9, 10, 5
. A better understanding of protist metabolic versatility in the simple dry valley lake food web will aid in the development of models for the role of protists in the global carbon cycle.
We employed an enrichment culture approach to isolate potentially phototrophic and mixotrophic protists from Lake Bonney. Sampling depths in the water column were chosen based on the location of primary production maxima and protist phylogenetic diversity 4, 11
, as well as variability in major abiotic factors affecting protist trophic modes: shallow sampling depths are limited for major nutrients, while deeper sampling depths are limited by light availability. In addition, lake water samples were supplemented with multiple types of growth media to promote the growth of a variety of phototrophic organisms.
RubisCO catalyzes the rate limiting step in the Calvin Benson Bassham (CBB) cycle, the major pathway by which autotrophic organisms fix inorganic carbon and provide organic carbon for higher trophic levels in aquatic and terrestrial food webs 12
. In this study, we applied a radioisotope assay modified for filtered samples 13
to monitor maximum carboxylase activity as a proxy for carbon fixation potential and metabolic versatility in the Lake Bonney enrichment cultures.
Microbiology, Issue 62, Antarctic lake, McMurdo Dry Valleys, Enrichment cultivation, Microbial eukaryotes, RubisCO
Electrochemotherapy of Tumours
Institutions: Institute of Oncology Ljubljana, University of Ljubljana.
Electrochemotherapy is a combined use of certain chemotherapeutic drugs and electric pulses applied to the treated tumour nodule. Local application of electric pulses to the tumour increases drug delivery into cells, specifically at the site of electric pulse application. Drug uptake by delivery of electric pulses is increased for only those chemotherapeutic drugs whose transport through the plasma membrane is impeded. Among many drugs that have been tested so far, bleomycin and cisplatin found their way from preclinical testing to clinical use. Clinical data collected within a number of clinical studies indicate that approximately 80% of the treated cutaneous and subcutaneous tumour nodules of different malignancies are in an objective response, from these, approximately 70% in complete response after a single application of electrochemotherapy. Usually only one treatment is needed, however, electrochemotherapy can be repeated several times every few weeks with equal effectiveness each time. The treatment results in an effective eradication of the treated nodules, with a good cosmetic effect without tissue scarring.
Medicine, Issue 22, electrochemotherapy, electroporation, cisplatin, bleomycin, malignant tumours, cutaneous lesions
Micro-masonry for 3D Additive Micromanufacturing
Institutions: University of Illinois at Urbana-Champaign.
Transfer printing is a method to transfer solid micro/nanoscale materials (herein called ‘inks’) from a substrate where they are generated to a different substrate by utilizing elastomeric stamps. Transfer printing enables the integration of heterogeneous materials to fabricate unexampled structures or functional systems that are found in recent advanced devices such as flexible and stretchable solar cells and LED arrays. While transfer printing exhibits unique features in material assembly capability, the use of adhesive layers or the surface modification such as deposition of self-assembled monolayer (SAM) on substrates for enhancing printing processes hinders its wide adaptation in microassembly of microelectromechanical system (MEMS) structures and devices. To overcome this shortcoming, we developed an advanced mode of transfer printing which deterministically assembles individual microscale objects solely through controlling surface contact area without any surface alteration. The absence of an adhesive layer or other modification and the subsequent material bonding processes ensure not only mechanical bonding, but also thermal and electrical connection between assembled materials, which further opens various applications in adaptation in building unusual MEMS devices.
Physics, Issue 90, Micro-masonry, microassembly, transfer printing, dry adhesives, additive manufacturing, printed processes, microfabrication, inks, microelectromechanical system (MEMS)
Setting-up an In Vitro Model of Rat Blood-brain Barrier (BBB): A Focus on BBB Impermeability and Receptor-mediated Transport
Institutions: VECT-HORUS SAS, CNRS, NICN UMR 7259.
The blood brain barrier (BBB) specifically regulates molecular and cellular flux between the blood and the nervous tissue. Our aim was to develop and characterize a highly reproducible rat syngeneic in vitro
model of the BBB using co-cultures of primary rat brain endothelial cells (RBEC) and astrocytes to study receptors involved in transcytosis across the endothelial cell monolayer. Astrocytes were isolated by mechanical dissection following trypsin digestion and were frozen for later co-culture. RBEC were isolated from 5-week-old rat cortices. The brains were cleaned of meninges and white matter, and mechanically dissociated following enzymatic digestion. Thereafter, the tissue homogenate was centrifuged in bovine serum albumin to separate vessel fragments from nervous tissue. The vessel fragments underwent a second enzymatic digestion to free endothelial cells from their extracellular matrix. The remaining contaminating cells such as pericytes were further eliminated by plating the microvessel fragments in puromycin-containing medium. They were then passaged onto filters for co-culture with astrocytes grown on the bottom of the wells. RBEC expressed high levels of tight junction (TJ) proteins such as occludin, claudin-5 and ZO-1 with a typical localization at the cell borders. The transendothelial electrical resistance (TEER) of brain endothelial monolayers, indicating the tightness of TJs reached 300 ohm·cm2
on average. The endothelial permeability coefficients (Pe) for lucifer yellow (LY) was highly reproducible with an average of 0.26 ± 0.11 x 10-3
cm/min. Brain endothelial cells organized in monolayers expressed the efflux transporter P-glycoprotein (P-gp), showed a polarized transport of rhodamine 123, a ligand for P-gp, and showed specific transport of transferrin-Cy3 and DiILDL across the endothelial cell monolayer. In conclusion, we provide a protocol for setting up an in vitro
BBB model that is highly reproducible due to the quality assurance methods, and that is suitable for research on BBB transporters and receptors.
Medicine, Issue 88, rat brain endothelial cells (RBEC), mouse, spinal cord, tight junction (TJ), receptor-mediated transport (RMT), low density lipoprotein (LDL), LDLR, transferrin, TfR, P-glycoprotein (P-gp), transendothelial electrical resistance (TEER),
Unraveling the Unseen Players in the Ocean - A Field Guide to Water Chemistry and Marine Microbiology
Institutions: San Diego State University, University of California San Diego.
Here we introduce a series of thoroughly tested and well standardized research protocols adapted for use in remote marine environments. The sampling protocols include the assessment of resources available to the microbial community (dissolved organic carbon, particulate organic matter, inorganic nutrients), and a comprehensive description of the viral and bacterial communities (via direct viral and microbial counts, enumeration of autofluorescent microbes, and construction of viral and microbial metagenomes). We use a combination of methods, which represent a dispersed field of scientific disciplines comprising already established protocols and some of the most recent techniques developed. Especially metagenomic sequencing techniques used for viral and bacterial community characterization, have been established only in recent years, and are thus still subjected to constant improvement. This has led to a variety of sampling and sample processing procedures currently in use. The set of methods presented here provides an up to date approach to collect and process environmental samples. Parameters addressed with these protocols yield the minimum on information essential to characterize and understand the underlying mechanisms of viral and microbial community dynamics. It gives easy to follow guidelines to conduct comprehensive surveys and discusses critical steps and potential caveats pertinent to each technique.
Environmental Sciences, Issue 93, dissolved organic carbon, particulate organic matter, nutrients, DAPI, SYBR, microbial metagenomics, viral metagenomics, marine environment
Multimodal Optical Microscopy Methods Reveal Polyp Tissue Morphology and Structure in Caribbean Reef Building Corals
Institutions: University of Illinois at Urbana-Champaign, University of Illinois at Urbana-Champaign, University of Illinois at Urbana-Champaign.
An integrated suite of imaging techniques has been applied to determine the three-dimensional (3D) morphology and cellular structure of polyp tissues comprising the Caribbean reef building corals Montastraeaannularis
and M. faveolata
. These approaches include fluorescence microscopy (FM), serial block face imaging (SBFI), and two-photon confocal laser scanning microscopy (TPLSM). SBFI provides deep tissue imaging after physical sectioning; it details the tissue surface texture and 3D visualization to tissue depths of more than 2 mm. Complementary FM and TPLSM yield ultra-high resolution images of tissue cellular structure. Results have: (1) identified previously unreported lobate tissue morphologies on the outer wall of individual coral polyps and (2) created the first surface maps of the 3D distribution and tissue density of chromatophores and algae-like dinoflagellate zooxanthellae
endosymbionts. Spectral absorption peaks of 500 nm and 675 nm, respectively, suggest that M. annularis
and M. faveolata
contain similar types of chlorophyll and chromatophores. However, M. annularis
and M. faveolata
exhibit significant differences in the tissue density and 3D distribution of these key cellular components. This study focusing on imaging methods indicates that SBFI is extremely useful for analysis of large mm-scale samples of decalcified coral tissues. Complimentary FM and TPLSM reveal subtle submillimeter scale changes in cellular distribution and density in nondecalcified coral tissue samples. The TPLSM technique affords: (1) minimally invasive sample preparation, (2) superior optical sectioning ability, and (3) minimal light absorption and scattering, while still permitting deep tissue imaging.
Environmental Sciences, Issue 91, Serial block face imaging, two-photon fluorescence microscopy, Montastraea annularis, Montastraea faveolata, 3D coral tissue morphology and structure, zooxanthellae, chromatophore, autofluorescence, light harvesting optimization, environmental change
In Situ SIMS and IR Spectroscopy of Well-defined Surfaces Prepared by Soft Landing of Mass-selected Ions
Institutions: Pacific Northwest National Laboratory.
Soft landing of mass-selected ions onto surfaces is a powerful approach for the highly-controlled preparation of materials that are inaccessible using conventional synthesis techniques. Coupling soft landing with in situ
characterization using secondary ion mass spectrometry (SIMS) and infrared reflection absorption spectroscopy (IRRAS) enables analysis of well-defined surfaces under clean vacuum conditions. The capabilities of three soft-landing instruments constructed in our laboratory are illustrated for the representative system of surface-bound organometallics prepared by soft landing of mass-selected ruthenium tris(bipyridine) dications, [Ru(bpy)3
(bpy = bipyridine), onto carboxylic acid terminated self-assembled monolayer surfaces on gold (COOH-SAMs). In situ
time-of-flight (TOF)-SIMS provides insight into the reactivity of the soft-landed ions. In addition, the kinetics of charge reduction, neutralization and desorption occurring on the COOH-SAM both during and after ion soft landing are studied using in situ
Fourier transform ion cyclotron resonance (FT-ICR)-SIMS measurements. In situ
IRRAS experiments provide insight into how the structure of organic ligands surrounding metal centers is perturbed through immobilization of organometallic ions on COOH-SAM surfaces by soft landing. Collectively, the three instruments provide complementary information about the chemical composition, reactivity and structure of well-defined species supported on surfaces.
Chemistry, Issue 88, soft landing, mass selected ions, electrospray, secondary ion mass spectrometry, infrared spectroscopy, organometallic, catalysis
A Guided Materials Screening Approach for Developing Quantitative Sol-gel Derived Protein Microarrays
Institutions: McMaster University .
Microarrays have found use in the development of high-throughput assays for new materials and discovery of small-molecule drug leads. Herein we describe a guided material screening approach to identify sol-gel based materials that are suitable for producing three-dimensional protein microarrays. The approach first identifies materials that can be printed as microarrays, narrows down the number of materials by identifying those that are compatible with a given enzyme assay, and then hones in on optimal materials based on retention of maximum enzyme activity. This approach is applied to develop microarrays suitable for two different enzyme assays, one using acetylcholinesterase and the other using a set of four key kinases involved in cancer. In each case, it was possible to produce microarrays that could be used for quantitative small-molecule screening assays and production of dose-dependent inhibitor response curves. Importantly, the ability to screen many materials produced information on the types of materials that best suited both microarray production and retention of enzyme activity. The materials data provide insight into basic material requirements necessary for tailoring optimal, high-density sol-gel derived microarrays.
Chemistry, Issue 78, Biochemistry, Chemical Engineering, Molecular Biology, Genetics, Bioengineering, Biomedical Engineering, Chemical Biology, Biocompatible Materials, Siloxanes, Enzymes, Immobilized, chemical analysis techniques, chemistry (general), materials (general), spectroscopic analysis (chemistry), polymer matrix composites, testing of materials (composite materials), Sol-gel, microarray, high-throughput screening, acetylcholinesterase, kinase, drug discovery, assay
Highly Resolved Intravital Striped-illumination Microscopy of Germinal Centers
Institutions: Leibniz Institute, Max-Delbrück Center for Molecular Medicine, Leibniz Institute, LaVision Biotec GmbH, Charité - University of Medicine.
Monitoring cellular communication by intravital deep-tissue multi-photon microscopy is the key for understanding the fate of immune cells within thick tissue samples and organs in health and disease. By controlling the scanning pattern in multi-photon microscopy and applying appropriate numerical algorithms, we developed a striped-illumination approach, which enabled us to achieve 3-fold better axial resolution and improved signal-to-noise ratio, i.e.
contrast, in more than 100 µm tissue depth within highly scattering tissue of lymphoid organs as compared to standard multi-photon microscopy. The acquisition speed as well as photobleaching and photodamage effects were similar to standard photo-multiplier-based technique, whereas the imaging depth was slightly lower due to the use of field detectors. By using the striped-illumination approach, we are able to observe the dynamics of immune complex deposits on secondary follicular dendritic cells – on the level of a few protein molecules in germinal centers.
Immunology, Issue 86, two-photon laser scanning microscopy, deep-tissue intravital imaging, germinal center, lymph node, high-resolution, enhanced contrast
Purification of Transcripts and Metabolites from Drosophila Heads
Institutions: University of Florida , University of Florida , University of Florida , University of Florida .
For the last decade, we have tried to understand the molecular and cellular mechanisms of neuronal degeneration using Drosophila
as a model organism. Although fruit flies provide obvious experimental advantages, research on neurodegenerative diseases has mostly relied on traditional techniques, including genetic interaction, histology, immunofluorescence, and protein biochemistry. These techniques are effective for mechanistic, hypothesis-driven studies, which lead to a detailed understanding of the role of single genes in well-defined biological problems. However, neurodegenerative diseases are highly complex and affect multiple cellular organelles and processes over time. The advent of new technologies and the omics age provides a unique opportunity to understand the global cellular perturbations underlying complex diseases. Flexible model organisms such as Drosophila
are ideal for adapting these new technologies because of their strong annotation and high tractability. One challenge with these small animals, though, is the purification of enough informational molecules (DNA, mRNA, protein, metabolites) from highly relevant tissues such as fly brains. Other challenges consist of collecting large numbers of flies for experimental replicates (critical for statistical robustness) and developing consistent procedures for the purification of high-quality biological material. Here, we describe the procedures for collecting thousands of fly heads and the extraction of transcripts and metabolites to understand how global changes in gene expression and metabolism contribute to neurodegenerative diseases. These procedures are easily scalable and can be applied to the study of proteomic and epigenomic contributions to disease.
Genetics, Issue 73, Biochemistry, Molecular Biology, Neurobiology, Neuroscience, Bioengineering, Cellular Biology, Anatomy, Neurodegenerative Diseases, Biological Assay, Drosophila, fruit fly, head separation, purification, mRNA, RNA, cDNA, DNA, transcripts, metabolites, replicates, SCA3, neurodegeneration, NMR, gene expression, animal model
Study of Phagolysosome Biogenesis in Live Macrophages
Institutions: Helmholtz Centre for Infection Research, National Institute for Medical Research.
Phagocytic cells play a major role in the innate immune system by removing and eliminating invading microorganisms in their phagosomes. Phagosome maturation is the complex and tightly regulated process during which a nascent phagosome undergoes drastic transformation through well-orchestrated interactions with various cellular organelles and compartments in the cytoplasm. This process, which is essential for the physiological function of phagocytic cells by endowing phagosomes with their lytic and bactericidal properties, culminates in fusion of phagosomes with lysosomes and biogenesis of phagolysosomes which is considered to be the last and critical stage of maturation for phagosomes. In this report, we describe a live cell imaging based method for qualitative and quantitative analysis of the dynamic process of lysosome to phagosome content delivery, which is a hallmark of phagolysosome biogenesis. This approach uses IgG-coated microbeads as a model for phagocytosis and fluorophore-conjugated dextran molecules as a luminal lysosomal cargo probe, in order to follow the dynamic delivery of lysosmal content to the phagosomes in real time in live macrophages using time-lapse imaging and confocal laser scanning microscopy. Here we describe in detail the background, the preparation steps and the step-by-step experimental setup to enable easy and precise deployment of this method in other labs. Our described method is simple, robust, and most importantly, can be easily adapted to study phagosomal interactions and maturation in different systems and under various experimental settings such as use of various phagocytic cells types, loss-of-function experiments, different probes, and phagocytic particles.
Immunology, Issue 85, Lysosome, Phagosome, phagolysosome, live-cell imaging, phagocytes, macrophages
Preparing Silica Aerogel Monoliths via a Rapid Supercritical Extraction Method
Institutions: Union College, Union College.
A procedure for the fabrication of monolithic silica aerogels in eight hours or less via a rapid supercritical extraction process is described. The procedure requires 15-20 min of preparation time, during which a liquid precursor mixture is prepared and poured into wells of a metal mold that is placed between the platens of a hydraulic hot press, followed by several hours of processing within the hot press. The precursor solution consists of a 1.0:12.0:3.6:3.5 x 10-3
molar ratio of tetramethylorthosilicate (TMOS):methanol:water:ammonia. In each well of the mold, a porous silica sol-gel matrix forms. As the temperature of the mold and its contents is increased, the pressure within the mold rises. After the temperature/pressure conditions surpass the supercritical point for the solvent within the pores of the matrix (in this case, a methanol/water mixture), the supercritical fluid is released, and monolithic aerogel remains within the wells of the mold. With the mold used in this procedure, cylindrical monoliths of 2.2 cm diameter and 1.9 cm height are produced. Aerogels formed by this rapid method have comparable properties (low bulk and skeletal density, high surface area, mesoporous morphology) to those prepared by other methods that involve either additional reaction steps or solvent extractions (lengthier processes that generate more chemical waste).The rapid supercritical extraction method can also be applied to the fabrication of aerogels based on other precursor recipes.
Chemistry, Issue 84, Aerogel fabrication, Silica aerogels, Aerogel monoliths, Rapid supercritical extraction, Hot press, Tetramethylorthosilicate (TMOS)
Detection of the Genome and Transcripts of a Persistent DNA Virus in Neuronal Tissues by Fluorescent In situ Hybridization Combined with Immunostaining
Institutions: CNRS UMR 5534, Université de Lyon 1, LabEX DEVweCAN, CNRS UPR 3296, CNRS UMR 5286.
Single cell codetection of a gene, its RNA product and cellular regulatory proteins is critical to study gene expression regulation. This is a challenge in the field of virology; in particular for nuclear-replicating persistent DNA viruses that involve animal models for their study. Herpes simplex virus type 1 (HSV-1) establishes a life-long latent infection in peripheral neurons. Latent virus serves as reservoir, from which it reactivates and induces a new herpetic episode. The cell biology of HSV-1 latency remains poorly understood, in part due to the lack of methods to detect HSV-1 genomes in situ
in animal models. We describe a DNA-fluorescent in situ
hybridization (FISH) approach efficiently detecting low-copy viral genomes within sections of neuronal tissues from infected animal models. The method relies on heat-based antigen unmasking, and directly labeled home-made DNA probes, or commercially available probes. We developed a triple staining approach, combining DNA-FISH with RNA-FISH and immunofluorescence, using peroxidase based signal amplification to accommodate each staining requirement. A major improvement is the ability to obtain, within 10 µm tissue sections, low-background signals that can be imaged at high resolution by confocal microscopy and wide-field conventional epifluorescence. Additionally, the triple staining worked with a wide range of antibodies directed against cellular and viral proteins. The complete protocol takes 2.5 days to accommodate antibody and probe penetration within the tissue.
Neuroscience, Issue 83, Life Sciences (General), Virology, Herpes Simplex Virus (HSV), Latency, In situ hybridization, Nuclear organization, Gene expression, Microscopy
A Gradient-generating Microfluidic Device for Cell Biology
Institutions: Brigham and Women's Hospital.
The fabrication and operation of a gradient-generating microfluidic device for studying cellular behavior is described. A microfluidic platform is an enabling experimental tool, because it can precisely manipulate fluid flows, enable high-throughput experiments, and generate stable soluble concentration gradients. Compared to conventional gradient generators, poly(dimethylsiloxane) (PDMS)-based microfluidic devices can generate stable concentration gradients of growth factors with well-defined profiles. Here, we developed simple gradient-generating microfluidic devices with three separate inlets. Three microchannels combined into one microchannel to generate concentration gradients. The stability and shape of growth factor gradients were confirmed by fluorescein isothyiocyanate (FITC)-dextran with a molecular weight similar to epidermal growth factor (EGF). Using this microfluidic device, we demonstrated that fibroblasts exposed to concentration gradients of EGF migrated toward higher concentrations. The directional orientation of cell migration and motility of migrating cells were quantitatively assessed by cell tracking analysis. Thus, this gradient-generating microfluidic device might be useful for studying and analyzing the behavior of migrating cells.
Issue 7, Cell Biology, tissue engineering, microfluidic, cell migration, gradient
A Microfluidic Device with Groove Patterns for Studying Cellular Behavior
Institutions: Brigham and Women's Hospital.
We describe a microfluidic device with microgrooved patterns for studying cellular behavior. This microfluidic platform consists of a top fluidic channel and a bottom microgrooved substrate. To fabricate the microgrooved channels, a top poly(dimethylsiloxane) (PDMS) mold containing the impression of the microfluidic channels was aligned and bonded to a microgrooved substrate. Using this device, mouse fibroblast cells were immobilized and patterned within microgrooved substrates (25, 50, 75, and 100 μm wide). To study apoptosis in a microfluidic device, media containing hydrogen peroxide, Annexin V, and propidium iodide was perfused into the fluidic channel for 2 hours. We found that cells exposed to the oxidative stress became apoptotic. These apoptotic cells were confirmed by Annexin V that bound to phosphatidylserine at the outer leaflet of the plasma membrane during the apoptosis process. Using this microfluidic device with microgrooved patterns, the apoptosis process was observed in real-time and analyzed by using an inverted microscope containing an incubation chamber (37°C, 5% CO2
). Therefore, this microfluidic device incorporated with microgrooved substrates could be useful for studying the cellular behavior and performing high-throughput drug screening.
Issue 7, Cell Biology, tissue engineering, microfluidic, apoptosis
Patterning of Embryonic Stem Cells Using the Bio Flip Chip
Institutions: MIT - Massachusetts Institute of Technology, MIT - Massachusetts Institute of Technology.
Cell-cell interactions consisting of diffusible signaling and cell-cell contact (juxtacrine signaling) are important in numerous biological processes such as tumor growth, stem cell differentiation, and stem cell self-renewal. A number of methods currently exist to modulate cell signaling in vitro. One method of modulating the total amount of diffusible signaling is to vary the cell seeding density during culture. Due to the random nature of cell seeding, this results in considerable variation in the actual cell-cell spacing and amount of cell-cell contact, and cannot prescribe the local environment. A more specific approach for modulating cell signaling is to use molecular inhibitors or genetic approaches to knock down specific signaling proteins, but both of these methods are best suited to manipulating small numbers of molecules. Here, we demonstrate a new approach to modulating cell-cell signaling that modulates the local environment of a cluster of cells by placing different numbers of cells at desired locations on a substrate. This method provides a complementary way to control the local diffusible and juxtacrine signaling between cells. Our method makes use of the Bio Flip Chip (BFC), a microfabricated silicone chip containing hundreds-to-thousands of microwells, each sized to hold either a single cell or small numbers of cells. We load the chip with cells simply by pipetting them onto the array of wells and washing unloaded cells off the array. The chip is then flipped onto a substrate, whereby the cells fall out of the wells and onto the substrate, maintaining their patterning. After the cells have attached, the chip can be removed (or left on). This approach to cell patterning is unique in that it: 1) doesn't alter the chemistry of the substrate, thus allowing cells to proliferate and migrate; 2) allows patterning onto any substrate, including tissue-culture polystyrene, glass, matrigel, and even feeder cell layers; and 3) is compatible with traditional microcontact printing, allowing the creation of extracellular matrix islands with cells placed inside those islands. In this video, we demonstrate the patterning of mouse embryonic stem cells onto tissue-culture polystyrene using the BFC.
Cellular Biology,Issue 8, tissue engineering, stem cells, patterning, bioengineering, signaling, diffusible, autocrine, juxtacrine | <urn:uuid:57db1625-da7c-4ade-a81b-25f0d4d473ac> | CC-MAIN-2017-47 | https://www.jove.com/visualize/abstract/19829693/genome-wide-transcriptome-analyses-silicon-metabolism-phaeodactylum | 2017-11-22T15:16:20Z | s3://commoncrawl/crawl-data/CC-MAIN-2017-47/segments/1510934806609.33/warc/CC-MAIN-20171122141600-20171122161600-00078.warc.gz | en | 0.893787 | 12,021 | [
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- 7 years ago
About the show
In our second ever podcast, we talk localism, markets, wine and cheese. Thanks to our guests, Joan Jones and Gwilim Owen, who enjoyed pot roasted hoggett; laverbread, leek and carrot cutlets; celeriac and potato au gratin; and a local cheese board.
Remember, you can get in contact if you’d like to share any recipes or indeed, would like to Share The Table with us.
Facebook - http://www.facebook.com/ShareTheTable
Twitter - http://twitter.com/ShareTheTable
Mail - [email protected] | <urn:uuid:c9998999-0b5c-4dea-805d-5d9f23838bd2> | CC-MAIN-2017-47 | https://www.mixcloud.com/sharethetable/share-the-table-ep02-say-cheese/ | 2017-11-22T14:55:12Z | s3://commoncrawl/crawl-data/CC-MAIN-2017-47/segments/1510934806609.33/warc/CC-MAIN-20171122141600-20171122161600-00078.warc.gz | en | 0.839261 | 136 | [
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Here it is. The time has come. The countdown is over and now it is cleanse time. Almost 29 years in to life and I'm finally doing a cleanse. What is this cleanse I speak of, you ask? Well, I'm doing the 'Standard Process Purification/Detoxification' program which focuses on eating real, organic food (fruits, veggies wild rice for the first 11 days, adding deep sea fish and chicken for the last 10 days) while taking whole food supplements to support detoxification of the liver, kidneys and intestines. I have to thank my friend and fellow detox buddy Sarah for talking me in to this (I thank her now, but we'll see if I'm still thankful a few days in!). I may not have ever attempted something like this by myself.
For 10 days (of the 21) I will essentially be a vegan. No gluten, caffeine, alcohol or dairy is allowed the entire program. For the first 10 days my eating will consist entirely of veggies, fruits and a very limited amount of rice (1/2 cup). It is a bit intimidating but something I'm willing to do because what I eat has changed so much over the last year. I will not be experiencing the negative effects of withdrawing from caffeine, processed foods or sugar because I eat very little, if any. If you are interested in the program and following along with me (and my detox buddy!) check out the .pdf of the entire program here. I will be having a rice protein mixture to blend in to my fruit smoothies (twice a day) so I don't anticipate being terribly hungry but we'll see.
First, you have to find a standard process kit - normally they are only supplied by nutritionists, chiropractors etc. But you can find them on Ebay or Amazon for around $200. You get everything you need for the 21 days and a nice booklet about the program including the food journal. You'll also need a blender for smoothies, which I had to purchase.
I bought some detox lemon tea as I can't drink green tea or any other tea with caffeine in it during the 21 days. It's highly recommended to taper off processed foods, sugar and caffeine prior to starting to avoid headaches. I had already pretty much stopped drinking coffee already (save one latte/week) and cut myself off from green tea two days before the start of the cleanse with no headaches. I don't normally eat processed foods or anything with refined sugars so it was an easy one there. I can see how it would be a horrible first few days of the cleanse if you try to quit everything at once - I heard that sugar is just as addictive as some illegal drugs (or more so) but I'm at a good point nutritionally to start this cleanse where I hope not to be miserable from withdrawal.
I started shopping a few days before, stocked up on wild rice, brown rice, frozen blackberries, mangoes, strawberries, blueberries, raspberries, green beans (all organic, of course) to have for my smoothies. Then came the day before the cleanse and it was really time to load up on the veggies. In the last year I developed a love for almost everything green. I don't like mushrooms, however, but there are plenty of green veggies that I LOVE. I am one of those people who prefers spinach over iceberg lettuce for a salad. I think Kale is one of my all-time favorites. I decided to shop for only a few days at a time so everything would be fresh. In a perfect world I'd love to be able to shop at open-air markets each day for my food like they do in so many other countries. Ideally I'd like to stop shopping at grocery stores all together and depend on local farms mostly for my food but that's a whole other thing to tackle. Anyway, I'm loaded up on veggies, fruits, high-quality oils, rice and am ready to go. Here's what my spread looked like on day 1:
Prepare yourself as I walk you through my journey, I'm providing advanced apologies for any cleanse-induced rants that may happen. Read along, if you dare.
I've been told the key to this cleanse/detox is preperation. So I packed my breakfast and lunch smoothie ingredients and a snack in containers for the work day. I woke up pretty stoked to start, actually munching on some raw kale to curb my hunger until I made my shake. The deal with this cleanse is you have to eat twice as many veggies as fruit, though they don't limit you on intake.
Breakfast Smoothie: 1 cup fruit (strawberry, rasberry, blackberry), 1 cup veggies (kale), 2 scoops SP Complete
Snacks: Carrots, Apple
Lunch: HUGE SALAD (meatless, dairy-free w/vinaigrette), Smoothie with 1 cup spinach, 1/2 cup mango, 1/2 cup peaches & fresh ginger
Dinner: Stirfry with garlic, onion, olive oil, wild rice, asparagus, green beens & carrots
The shake was OK although I'm a big fan of kale or spinach in smoothies anyway. The protein powder came off of chalky to me, which I also expected. Not too bad, wishing it was a little sweeter but completely drinkable. Also snacked on a cup of carrots too, might as well keep eating while I have the appetite. Downed the supplements with water at each meal (10 capsules total of organic whole food powders/herbs, 3 times a day) which was a bit difficult. Ginger tastes like sunshine. It's probably going to end up in all my smoothies from now on.
Day one I was hungry pretty much every 90 minutes - likely because organic fruits and veggies are so easy for your body to process so they would move out of my stomach quickly. Hunger is an interesting feeling. I didn't experience it too often on my current healthy eating plan (prior to the cleanse) because I always ate a fair amount of animal protein with each meal. Eating only fruits and veggies for most of the day was difficult, made me hungry at multiple points in the day and made me look forward to my one meal with rice that was allowed. I think for me, even though I do eat pretty healthy, it's important while going through this cleanse to recognize the feeling of hunger. Because I am drinking so much water I know the feeling is not thirst. And the opposite is true - I need to know what a 'full' feeling is for me. Not stuffed, but satisfied. During day one I noticed that drinking the smoothies did make me full but for a short period of time. I could quickly satisfy my hunger with some carrots, apple or other veggies.
Dinner tasted amazing. It was interesting how just the tiny bit of fat (olive oil) made the meal so tasty. For dessert I made myself a little frozen banana sorbet (just 1/2 frozen banana with a little water run through a blender) and that was also delicious. Day one went really well, I felt great at the end of it and followed all of the cleanse rules. In fact I drank 40 more ounces of water than recommended.
I slept so well last night. Normally I sleep well but last night I don't recall moving or tossing around at all. Just like a rock. Got out of bed early and ready for the day, stepped on the scale first thing - I lost 1/2 a pound in day one. That's pretty reasonable considering I don't really have a lot of detoxing to do (clean eating for the past year or so) and that I only ate about 700 calories yesterday. Half a pound makes perfect sense. Day 2 is likely to look a lot like Day 1. I figured out yesterday that I was eating too little fruit (the suggested fruit serving sizes are generally bigger than the veggie serving sizes) so my shake this morning was huge and thick like sorbet, really yummy. I honestly did not taste the powder.
Breakfast : Banana, Blueberry, Strawberry & Spinach Smoothie
Snack: Carrots, Apple, frozen banana sorbet
Lunch: Mango Peach Ginger & Spinach Smoothie, mixed green salad w/balsamic vinaigrette
Dinner : 1/2 cup rice stirfry w/green beans and broccoli
At some point during my walk today I contemplated vegetarianism. I thought how respectable it was to be on a nutrition plan just like this everyday. A deliberate effort to not indulge in animal meat (or products for vegan). I realized having meat was an indulgence, really, or cheese or milk. They are things other live beings have to give to us. The last two days have really opened up my eyes to vegetarianism as a manageable lifestyle - I had not been too hungry in the last two days and was pretty full of energy, quite honestly. Maybe after this I will start eating less meat...so many other things are indulgences like coffee or alcohol. I am already two days in to this cleanse and trying to better define want vs. need when it comes to what I am eating or drinking, which is EXACTLY why I am doing it.
I felt really good the whole day and was never too hungry. Having a nice big warm meal at the end of the day (even if it was just a little bit of rice and a ton of veggies) was the perfect ending to the day.
DAY 3 :
Again last night I slept very well. Usually Landon gets here around 8am and we nap for an additional hour or two. He got here at 7:30 this morning and I honestly didn't feel tired at all. I do want to note this morning I felt like I had done a really good ab workout and I'm sure that's due to what's going on in my stomach and digestive system right now.
Got on the scale and I lost a pound and a half yesterday (2lbs in 2 days)! Now, I already know that you're thinking it's just water weight or effects of flushing things out of my digestive system but I know it's not either of those things. I am drinking so much water, up to 100oz/day to the point where I thought drinking that much water would make me weigh more. And another reason; this is a cleanse not a flush. There are no strange things coming out of me at a rapid pace.
Now a note about exercise during the 21 days. Standard Process recommends not doing any high-intensity workouts during the three weeks. I have been walking a few miles a day but I'm going a little crazy without my workouts - and I feel great. Today on top of some good house cleaning I did a bit of strength training to test the waters. Still felt fine. I will probably try to go back to my workout classes on Monday, I figure if I feel really fatigued I can just cut out of the workout early.
Breakfast: Kiwi Apple Spinach Smoothie
Snacks: Carrots, Banana sorbet
Lunch : Banana Orange Pineapple Ginger Spinach Smoothie, spicy kale & green bean salad
Dinner : Sweet potato (baked) spicy fries, steamed broccoli, 1/2 cup rice
Here is my breakfast smoothie and the kale & bean salad (sauteed with onions, red pepper flakes & chili powder). Apple and Kiwi wasn't my favorite combo, the smoothies with mango or berries have been better. I liked the extra spice of the kale and bean salad, I had been having a mixed green salad for lunch so this was a nice change.
Taking the supplements (30 capsules/day) is not getting any easier but I find taking them with lemon water curbs the weird taste at least. Another good day on this cleanse. I'm satisfied with what I'm eating and really not that hungry.
Weighing myself at the beginning of day 4i lost another .8 lbs, down 2.8 total in three days - I'd say we can round that to 3! | <urn:uuid:5f237b15-0636-4004-9629-eff8377d8ff4> | CC-MAIN-2017-47 | http://chapter2lifelovemusic.blogspot.com/2013/04/days-1-3-of-uber-long-21-day-cleanse.html | 2017-11-23T09:20:06Z | s3://commoncrawl/crawl-data/CC-MAIN-2017-47/segments/1510934806768.19/warc/CC-MAIN-20171123085338-20171123105338-00078.warc.gz | en | 0.971693 | 2,521 | [
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We receive many inquiries from out-of-town investors who are enthusiast about the idea of buying a Florida home to use as an investment. Some of them are looking to diversify their assets beyond just their home country or region; some see it as a way to get a foothold in the Florida real estate market in advance of moving to the Sunshine State themselves when they retire; others simply like the idea of owning property in a place that continues to grow and attract more residents.
We are able to report our experiences over the past few years, where home prices have recovered significantly since the downturn that happened almost 10 years ago. One big factor in this relates to the long-term rental market: there is a high demand for homes from prospective renters because a lot of residents lost their own property in the downturn and are still unable to buy, so they have to rent. Many investors have taken advantage of this high demand by moving into real estate but even so, the demand from renters seems to be a lot higher than the supply. Long-term rents are increasing and not falling, and this will have an impact on short-term rental home prices too.
A recent article adds more background to the situation that is common in many parts of the USA and Orlando is mentioned as one example of what’s going on:
The shift has been vivid over the past five years in the Piedmont Park neighborhood of Apopka, a former agricultural hub now crowded with housing developments. Where one in 10 homes was once a rental, now more than a third are. Many are owned by Wall Street investment firms that bought them out of foreclosure at deep discounts.
You can read the whole thing here.
To understand more about long-term versus short-term rental property and which one may be best for you, send us your details by using the form below. | <urn:uuid:dba01109-2bae-4663-a731-1b64e130b670> | CC-MAIN-2017-47 | http://buying-florida-property.co.uk/category/uncategorized/ | 2017-11-24T03:29:07Z | s3://commoncrawl/crawl-data/CC-MAIN-2017-47/segments/1510934807084.8/warc/CC-MAIN-20171124031941-20171124051941-00078.warc.gz | en | 0.971126 | 375 | [
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Thank you Jeff Novick for your sensible healthy eating placemat (2013). If you don’t already know Jeff his website has tons of sensible information and will help you revitalise your eating regime.
The Five Pillars of Healthy Eating:
1) Plant-Centred– Centre your plate and your diet around minimally processed plant foods (fruits, vegetables, starchy vegetables, roots/tubers, intact whole grains, and legumes (beans, peas & lentils).
2) Minimally Processed – Enjoy foods as close to “as grown in nature” with minimal processing that does not detract from the nutritional value &/or add in any harmful components.
3) Calorie Dilute – Follow the principles of calorie density choosing foods that are calorie adequate, satiating and nutrient sufficient.
4) Low S-O-S – Avoid/minimize the use of added Salts/sodium, Oils/Fats and Sugars/sweeteners
5) Variety – Consume a variety of foods in each of the recommended food groups. | <urn:uuid:e1281a5e-927d-4524-bfa0-dec9bbfeb6b8> | CC-MAIN-2017-47 | http://www.thegentlekitchen.com/?page_id=3493 | 2017-11-24T03:29:23Z | s3://commoncrawl/crawl-data/CC-MAIN-2017-47/segments/1510934807084.8/warc/CC-MAIN-20171124031941-20171124051941-00078.warc.gz | en | 0.87553 | 224 | [
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I use cscope integrated to VIM to navidate a large code base. I can use the :E command to navigate the directory structure. But, how do I open a shell on a specific directory where I navigated to?
I came across
With Vim, there are always multiple ways to suit your needs.
You could for example create a shortcut (called mapping) for the command you've found (and/or combine this with opening a shell):
:nnoremap <Leader>. :cd %:p:h<CR>
But this particular use case is so prevalent that there's actually an option for it:
As an alternative, some people always open Vim from the root directory of their current project; this ensures short (and consistent!) paths, too. There are even plugins that automatically
:cd to the project dir for you. | <urn:uuid:b6f2e257-5577-41dc-8fa9-a3cbfd808750> | CC-MAIN-2017-47 | https://codedump.io/share/GrdVxILRuAlc/1/vim---shell-working-directory-integration | 2017-11-24T04:29:53Z | s3://commoncrawl/crawl-data/CC-MAIN-2017-47/segments/1510934807084.8/warc/CC-MAIN-20171124031941-20171124051941-00078.warc.gz | en | 0.885512 | 176 | [
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Pittsburgh’s Best Kitchen Remodeling makes a speciality of kitchen transforming and restore. There are numerous sorts of home equipment to choose from when planning your renovation, from EnergyStar-licensed to customized built-in to high-finish store bought to price range outlet home equipment. An example is creatively using the prevailing kitchen footprint which saves cash on flooring, plumbing, and other minor expenses allowing money to be dispersed on bigger features of the kitchen transform.
Your type will lead you to the best kitchen cupboard selection , in terms of door style, end and/or color. This nice kitchen remodeling idea particularly considering the fact that most individuals take a look at the kitchen counter first. Whether or not you want to transform your kitchen to increase performance and luxury, or to reallocate the space to create a spot for family life, you are able to do wonderful things within any funds.
For those who’re relocating a sink or installing an entirely new one, professional assist can be mandatory, which might cost several hundred dollars. This is a helpful device to convey with you as you begin to discuss your challenge with an inside designer, the Kitchen Magic Experts, and contractor(s).
Moreover, practically 50 p.c of kitchens carried out by National Kitchen and Tub Association members had been priced between $20,000 and $forty nine,999, and one other 35 p.c cost between $50,000 and $ninety nine,999, in accordance with Jordan Zapalotosch of NKBA. Having both will help salespeople, suppliers and/or contractors to see more accurately what you want to create your kitchen design.
Then our kitchen designers get to work designing and building your dream kitchen. These inside specialists will allow you to whether or not you need a reworking mission, a kitchen makeover, or each. The kitchen is the heart of the house and it is usually the most commonly renovated room in the home. | <urn:uuid:688f4649-fcf1-4683-a884-c221c748a61f> | CC-MAIN-2017-47 | http://yhlwy.com/kitchen-remodeling-kitchen-remodel-designs.html | 2017-11-24T21:53:23Z | s3://commoncrawl/crawl-data/CC-MAIN-2017-47/segments/1510934808972.93/warc/CC-MAIN-20171124214510-20171124234510-00078.warc.gz | en | 0.938506 | 399 | [
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Golf is an outdoor sport so the weather should be a key factor when you decide to have some swings after a long hard work week. So the winter season in some countries will ruin your golfing day but this will not be the case for Vietnam. So what will be the best time to visit the lovely, golf – friendly country, Vietnam for some swings under the sun & cool breeze?
The best time to visit Vietnam is spring (February to April) and autumn (August to October). The temperatures are more moderate and rainfall is lighter. In spring, March and April have the lowest rainfall across all destinations and temperatures are pleasant, though still cool in the far north.
Please check out the map below for more information on the best time to visit on some famous tourist destinations in Vietnam
So plan your golf holiday now and book through us for receiving the best rate as well as customize your trip into your best golf trip in Vietnam ever.
Source : pictures & info from insiderjourneys.com.au/blog/ | <urn:uuid:c7c41973-c0a3-4c1e-b802-793ef30d42e3> | CC-MAIN-2017-47 | http://golftourismvietnam.com/best-time-for-visiting-vietnam/ | 2017-11-17T22:52:38Z | s3://commoncrawl/crawl-data/CC-MAIN-2017-47/segments/1510934804019.50/warc/CC-MAIN-20171117223659-20171118003659-00278.warc.gz | en | 0.929929 | 208 | [
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Preface: Our Call to Love
We are called to worship and be discipled corporately, and we are called to love and serve one another.
If we are one of Christ’s disciples, we are called to disciple and be discipled by others. Further, Scripture tells us that we each have primary responsibility for our brothers and sisters within our local covenanted body at JPBC. The leadership at JPBC is committed to creating a culture of personal discipleship. We envision a church where our members are actively involved in each others’ lives for the purpose of encouragement, correction, accountability, and showing each other grace and forgiveness in the gospel. The elders/pastors are called to equip and encourage the saints in this work for purposes of building up the entire body under the headship of Christ.
Our discipleship takes place through two main areas: through corporate worship and fellowship and through members' discipleship and care.
Discipleship takes place as we gather together to hear from God and care for one another in our corporate worship on Sunday morning, our focused studies in Sunday school, and in our fellowship time on Wednesday night. These three services are what the pastor likes to call the "corporate diet" of the church and provide a unique way to feed, encourage, and edify the body.
Expectations for Members
Sunday Morning Worship Service | Ordinances, singing with one another, hearing Scripture read, hearing Scripture preached, led in prayer
Our discipleship starts with our corporate worship service on the Lord's Day. This is the main course in our corporate diet where we gather to fulfill what God clearly prescribes for us in Scripture. The service centers on the preaching of God's Word as we all gather seeking to hear God speak to us. The preaching is expositional, Christ-centered, and focuses on applying the Word to our lives today.
The music is chosen to lead the congregation in praising God and is chosen to prepare us to hear the message. We incorporate different kinds of led prayers in the service such as prayers of praise, confession, thanksgiving, and repentance. The morning worship service is also where we participate in the ordinances of our Lord, Baptism and the Lord's Supper.
Wednesday Night Service | Corporate prayer for one another, adding/removing members, caring for members, fellowship, studying God’s Word together
Our Life Together is the Wednesday evening gathering that complements the Sunday worship and gives the church an opportunity to experience Christ in one another and learn together.
We will typically have a brief time of singing beginning at 6PM followed by a time of prayer, ending at 7:15PM.
Small Groups/Bible Studies (at least one) | Being watchful, loving one another, stirring one another toward good works, discipling, confronting, correcting, encouraging
Sunday School: The Sunday School hour precedes the worship service, starting at 9AM. These classes range from walking through a book of the Bible to a class that prays for the hour. The venue of Sunday School provides for more discussion and sharing time. Some classes are gender and age specific. Others are co-ed and open to all ages. We hope to offer a rotation of open classes that will include New and Old Testament studies, theological topics, counseling, discipleship, family and evangelism.
Volunteer (at least one) | Serving one another, loving one another
Children’s Sunday School | <urn:uuid:ffd38c03-c53d-4ddc-8c70-306849fef291> | CC-MAIN-2017-47 | http://jpbc.org/church-diet/ | 2017-11-17T23:11:29Z | s3://commoncrawl/crawl-data/CC-MAIN-2017-47/segments/1510934804019.50/warc/CC-MAIN-20171117223659-20171118003659-00278.warc.gz | en | 0.942155 | 709 | [
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What I appreciated about my Featured “Book of Color” Pick of the Day is that in The African Book of Names it is more than just a list of 5000 African names. There is a meaning behind each name and it is apparent that the author, Dr. Askhari Johnson Hodari, put a lot of time and attention into choosing each and every one of these names.
More than just a baby book, it is noted that many prominent people in history changed their names later in life. People such as Sojourner Truth, Muhammad Ali and Kareem Abdul-Jabbar all took names as adults.
Dr. Hodari is also one of the most sought-after experts on African names and for over 20 years has consulted in the naming and or renaming of hundreds of babies, children and adults.
Jeff Rivera is the author of “Forever My Lady” and the founder of GumboWriters.com | <urn:uuid:c60ee08d-74c3-4e5c-a931-5968b300c41c> | CC-MAIN-2017-47 | http://www.adweek.com/galleycat/dr-askhari-johnson-hodari/10533?red=as | 2017-11-18T00:05:59Z | s3://commoncrawl/crawl-data/CC-MAIN-2017-47/segments/1510934804019.50/warc/CC-MAIN-20171117223659-20171118003659-00278.warc.gz | en | 0.974863 | 193 | [
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There’s at least one moment every few days when I think to myself: “Teaching really is the best career in the world.” Sometimes this thought is spurred on when a student finally understands a concept. Other days it’s activated by the zing of a vibrant classroom discussion. Sometimes it comes from the intellectual buzz of making a tough text like Shakespeare accessible. But, in the case of this week… my teacher euphoria came from our DOUBLE SNOW DAY off from school!
Before diving into grading and planning today (yes, we still work during days off, but it’s a lot cozier in PJs), I couldn’t resist taking a walk around the neighborhood to capture the beauty of icy Boston. Come walk with me and check it out! Click each thumbnail to enlarge the photo. | <urn:uuid:f6465472-67f1-4441-a102-daf95f81ca27> | CC-MAIN-2017-47 | https://www.aroundtheworldl.com/2011/01/13/sparkling-photos-from-our-double-snow-day-in-boston/ | 2017-11-17T22:53:16Z | s3://commoncrawl/crawl-data/CC-MAIN-2017-47/segments/1510934804019.50/warc/CC-MAIN-20171117223659-20171118003659-00278.warc.gz | en | 0.943354 | 174 | [
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The World Social Work Day is organised by the International Federation of Social Workers (IFSW), the International Association of Schools of Social Work (IASSW), the United Nations Research Institute for Social Development (UNRISD) and the School of Social Work Geneva (HETS-Genève).
Sustainability represents one of the most revolutionary paradigm shifts of the 20th century, with consequences for social work, as it is closely related to relationships between human communities, their future and the environment.
World Social Work Day 2017 will explore the following questions: What can social work contribute and which competencies are needed to implement the UN Sustainable Development Goals (SDG)?
The event is an opportunity to exchange knowledge and strengthen synergies between the social work community, UN organisations and international NGOs. It is aimed at international civil servants, social work professionals, students and all others with an interest in the topic. | <urn:uuid:c30d000f-30c4-4c51-8375-def384fb013a> | CC-MAIN-2017-47 | http://socialprotection.org/connect/calendar/world-social-work-day-2017-what-can-social-work-contribute-and-which-competencies | 2017-11-18T17:27:53Z | s3://commoncrawl/crawl-data/CC-MAIN-2017-47/segments/1510934805008.39/warc/CC-MAIN-20171118171235-20171118191235-00278.warc.gz | en | 0.914534 | 183 | [
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This is a great gun overall. Accurate and powerful. Shoots through 1/2 inch plywood with no problem. Only shot about 50 rounds but I am impressed with how quiet it is. Although the manual says it will quiet down even more after a couple of hundred rounds.My only complaints are that the scope is cheaply made and the trigger pull is very long even though the adjustment bolt is tightened make it as short as it can be. This is fixed by installing a longer 3mm bolt instead of the factory one. Wish it had swivel studs and a picatinny rail instead of the dovetail for optics since it is more versatile and modern.Squirrels don't stand a chance. Great gun for the money.
NOTICE: All product prices, availability, and specifications
are subject to verification by their respective retailers. Ammogear acts as
an advertising vehicle for companies who legally sell guns and ammo on the
internet. As such, we are not the seller of record for these items, though
we may receive advertising fees from certain of our merchant partners. | <urn:uuid:be31b3e6-20b0-4f85-bc0a-3fabbe4a446f> | CC-MAIN-2017-47 | http://www.ammogear.com/_1/9/6/Crosman_Crusher_NP_22_Caliber_Break_Barrel_Air_Rifle_with_Scope_43989691.htm | 2017-11-18T17:58:08Z | s3://commoncrawl/crawl-data/CC-MAIN-2017-47/segments/1510934805008.39/warc/CC-MAIN-20171118171235-20171118191235-00278.warc.gz | en | 0.962302 | 223 | [
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WordPress UberMenu Sticky Extension adds the ability to make your UberMenu “sticky”, meaning it’ll stick to the same spot in the viewport as your users scroll down your page.
Sticky menus for WordPress allow your users easy access to your menu at all times, without having to scroll back to the top of the page to navigate to a different part of your site. The Sticky Menu Extension will stick your menu in place even if it doesn’t start off at the top of your site, and hold it there while your users browse your content.
Please note that as this is an extension, it will not do anything without UberMenu installed.
UberMenu Sticky Menu Extension Features
By default, UberMenu Sticky Menu Extension is automatically disabled for mobile devices (phones, iPods, etc), since overflow content on fixed position menus will become inaccessible on small screens. It will continue to function as a sticky menu on larger devices, such as iPads, however.
UberMenu Sticky does have an experimental option for displaying sticky menus on mobile, which may work with your theme. | <urn:uuid:7c00d53e-7437-4596-87a7-55b186e5c1bd> | CC-MAIN-2017-47 | https://dl-wordpress.com/ubermenu-sticky-menu-extension/ | 2017-11-18T17:20:22Z | s3://commoncrawl/crawl-data/CC-MAIN-2017-47/segments/1510934805008.39/warc/CC-MAIN-20171118171235-20171118191235-00278.warc.gz | en | 0.854698 | 230 | [
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Monday, May 20, 2013
We have been doing a lot of hiking lately now that weather is nice. We went to the hills on Quick Road in the cuyahoga valley national park.
The boys loved it. The hills were high and the boys loved walking up them. When we got back, the boys decided they wanted to roll down the hills. Joy and I watched from the top.
Wednesday, May 15, 2013
It has been long since an update. Shortly after the last post, the boys got a cold and we ended up being inside and chilling for a week.
After the boys got better, the boys really wanted to go somewhere. Joy thought the zoo. We thought there would be no one there and we would let the boys play in the rainforest till they got tired.
On the ride up, we heard a radio advertisement for "party for the planet" at the zoo. The place was packed. We ended up going to the zoo. We let the boys decide what to see. Jacob led around because he wanted to see monkeys, then elephants.
We went to the rainforest and we saw armadillos out. I was so excited but couldn't get the boys too interested. They were letting people pet them. I did not but I wish I did.
Jacob loved taking his time and seeing all the animals. Jasper like moving fast, looking at everything quickly.
It was a really fun day | <urn:uuid:4997b4fd-ba3c-4d70-be3b-9ddef774df24> | CC-MAIN-2017-47 | http://swellbeansprouts.blogspot.com/2013_05_01_archive.html | 2017-11-21T02:46:10Z | s3://commoncrawl/crawl-data/CC-MAIN-2017-47/segments/1510934806310.85/warc/CC-MAIN-20171121021058-20171121041058-00278.warc.gz | en | 0.984061 | 296 | [
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Why ePlus Shares Are Nonplussed Today
Although we don't believe in timing the market or panicking over market movements, we do like to keep an eye on big changes -- just in case they're material to our investing thesis.
What: Shares of ePlus have fallen as much as 10% today, and now sit at a 7% loss, following a third-quarter report that failed to live up to investor expectations for reasons that are hard to extrapolate.
So what: ePlus reported an 8% year-over-year increase in quarterly revenue, to $242.0 million, and a 3.3% increase in earnings, to $9.0 million. However, as a result of the company's December $2.50-per-share special dividend, diluted earnings per share fell to $1.05 against $1.07 per share the year before. This is the only true negative in the report (except for the possibility of shrinking margins), as ePlus maintains a higher cash balance of $42.2 million in its latest quarter, against the $33.8 million it held a year ago, in spite of the special dividend. Net income for the first three quarters of ePlus' fiscal 2013 increased 45%, nearly doubling either its 23.1% increase in revenue or its 22.1% increase in costs and expenses. The company also completed a number of projects early, which boosted deferred revenue significantly over the year-ago period.
Now what: There aren't any obvious negatives on the surface of this earnings report. Is Wall Street worried about the lack of issued guidance? Maybe it should start issuing its own guidance -- at present, no analysts cover this stock. Is the higher level of deferred revenue going to hurt ePlus' margins in the fourth quarter? The reasons for this drop are murky, which means that it might be time to take a closer look at this growing stock with the low 11.7 P/E.
Want more news and updates? Add ePlus to your Watchlist now.
The Motley Fool's chief investment officer has selected his number one stock for 2013. Find out which stock it is in our brand-new free report: "The Motley Fool's Top Stock for 2013." I invite you to take a copy, free for a limited time. Just click here to access the report and find out the name of this under-the-radar company.
The article Why ePlus Shares Are Nonplussed Today originally appeared on Fool.com.Fool contributor Alex Planes holds no financial position in any company mentioned here. Add him on Google+ or follow him on Twitter @TMFBiggles for more news and insights.The Motley Fool has no position in any of the stocks mentioned. Try any of our Foolish newsletter services free for 30 days. We Fools may not all hold the same opinions, but we all believe that considering a diverse range of insights makes us better investors. The Motley Fool has a disclosure policy.
Copyright © 1995 - 2013 The Motley Fool, LLC. All rights reserved. The Motley Fool has a disclosure policy. | <urn:uuid:56d3feaa-18ea-47ae-882c-c5c4276171f2> | CC-MAIN-2017-47 | https://www.aol.com/2013/02/07/why-eplus-shares-are-nonplussed-today/ | 2017-11-21T03:33:59Z | s3://commoncrawl/crawl-data/CC-MAIN-2017-47/segments/1510934806310.85/warc/CC-MAIN-20171121021058-20171121041058-00278.warc.gz | en | 0.956744 | 639 | [
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|Casting Time:||1 minute|
|Effect:||Visual figment that can extend for up to twenty 30-ft. cubes (S)|
|Duration:||Concentration plus 20 hours|
|Saving Throw:||Will disbelief (if interacted with)|
A spell developed with the delude seed creates the visual illusion of an object, creature, or force, as visualized by the caster. The caster can move the image within the limits of the size of the effect by concentrating (the image is otherwise stationary). The image disappears when struck by an opponent unless the caster causes the illusion to react appropriately. For an illusion that includes audible, olfactory, tactile, taste, and thermal aspects, increase the Spellcraft DC by +2 per extra aspect. Even realistic tactile and thermal illusions can’t deal damage, however. For each additional image to be created, increase the Spellcraft DC by +1. For an illusion that follows a script determined by the caster, increase the Spellcraft DC by +9. The figment follows the script without the caster having to concentrate on it. The illusion can include intelligible speech if desired. For an illusion that makes any area appear to be something other than it is, increase the Spellcraft DC by +4. Additional components, such as sounds, can be added as noted above. Concealing creatures requires additional spell development using this or other seeds. | <urn:uuid:073efa03-1583-47b2-bece-dffd83f998f2> | CC-MAIN-2017-47 | http://www.35srd.com/epic-rules/epicspellsintro/seed-descriptions/delude/ | 2017-11-21T21:16:25Z | s3://commoncrawl/crawl-data/CC-MAIN-2017-47/segments/1510934806426.72/warc/CC-MAIN-20171121204652-20171121224652-00278.warc.gz | en | 0.896576 | 290 | [
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The National Capital Area Gladney Family Association cordially invites you to our
9th annual Princess Ball….
"Curiouser and Curiouser"
Benefiting the Gladney Center for Adoption
Dining and Dancing for Dads and their Daughters
Saturday, March 4th 2017 from 6 -10pm
Westwood Country Club | <urn:uuid:2dc83123-8b88-4863-9f16-b7feca909e13> | CC-MAIN-2017-47 | https://iamgladney.org/civicrm/event/info?reset=1&id=16 | 2017-11-21T20:58:46Z | s3://commoncrawl/crawl-data/CC-MAIN-2017-47/segments/1510934806426.72/warc/CC-MAIN-20171121204652-20171121224652-00278.warc.gz | en | 0.904272 | 69 | [
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- Crowning Music of Awesome: The quartet Alla bella Despinetta starts the section and then the sisters join in.
- Crowning Moment of Awesome: Despina, the snarky, world-wise maid who laughs at the dramatic notions of love her mistresses have, is always ready for a laugh and takes everything in stride. Una donna a quindici anni is probably the best example; the music even swells up into a crescendo when she tells them to do as she says to reign like a queen with "you could" and "I'd like".
- Crowning Moment of Funny
Dorabella: Our fiances have left for war!Despina: So much the better for them: You'll see them return crowned with laurel.Fiordiligi: But they might be killed!Despina: Why then, so much the better for you!
- Cut Song: During rehearsals, Mozart cut Guiglielmo's long, showy Act 1 aria "Rivolgete a lui lo sguardo" and replaced it with a much shorter aria. "Rivolgete" was published separately, and some productions put it back into the opera for singers who want a Show Stopper.
- Values Dissonance
- Family-Unfriendly Aesop: Guys, all girls turn into sluts if you don't pay them enough attention. They're women, they can't help it: All Women Are Lustful.
- Alternately... it's unfair for men to expect women to stick to just one man, since no one expects that of men. Monogamy is unrealistic and you're better off the sooner you learn that. If the characters of half of the tragic operas had heard Despina's advice, they could have survived (e.g. Otello, Tosca, Don Jose...) | <urn:uuid:46236161-992f-4eb4-9a6b-11a7d9a704b9> | CC-MAIN-2017-47 | http://tvtropes.org/pmwiki/pmwiki.php/YMMV/CosiFanTutte | 2017-11-22T17:01:43Z | s3://commoncrawl/crawl-data/CC-MAIN-2017-47/segments/1510934806615.74/warc/CC-MAIN-20171122160645-20171122180645-00278.warc.gz | en | 0.941719 | 401 | [
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Since it is mid-April, and most of the comic strips at Comics Kingdom and GoComics.com are based in the United States, Comic Strip Master Command ordered quite a few comics about taxes. Most of those are simple grumbling, but the subject naturally comes around to arithmetic and calculation and sometimes even logic. Thus, this is a Tax Day edition, though it’s bookended with Mutt and Jeff.
Bud Fisher’s Mutt And Jeff (April 11) — a rerun rom goodness only knows when, and almost certainly neither written nor drawn by Bud Fisher at that point — recounts a joke that has the form of a word problem in which a person’s age is deduced from information about the age. It’s an old form, but jokes about cutting the Gordion knot are probably always going to be reliable. I’m reminded there’s a story of Thomas Edison giving a new hire, mathematician, the problem of working out the volume of a light bulb. Edison got impatient with the mathematician treating it as a calculus problem — the volume of a rotationally symmetric object like a bulb is the sort of thing you can do by the end of Freshman Calculus — and instead filling a bulb with water, pouring the water into a graduated cylinder, and reading it off that.
Sandra Bell-Lundy’s Between Friends (April 12) uses Calculus as the shorthand for “the hardest stuff you might have to deal with”. The symbols on the left-hand side are fair enough, although I’d think of them more as precalculus or linear algebra or physics, but they do parse well enough as long as I suppose that what sure looks like a couple of extraneous + signs are meant to refer to “t”. But “t” is a common enough variable in calculus problems, usually representing time, sometimes just representing “some parameter whose value we don’t really care about, but we don’t want it to be x”, and it looks an awful lot like a plus sign there too. On the right side, I have no idea what a root of forty minutes on a treadmill might be. It’s symbolic.
Brian Walker, Greg Walker, and Chance Browne’s Hi and Lois (April 12) depicts something nearly universal: the same kind of work can be a delight or a drudge, based on why you’re doing it. This is also why people try to insist mathematics is worth learning because it’s so practical. The assumption is that something practical must be desirable. Pointing out where mathematics is fun, or beautiful, is probably a better motivation.
Randy Glasbergen’s Glasbergen Cartoons (April 12) shows a guy complaining that if a decimal point is important, why isn’t it bigger? And it’s a fair question. As best I can work out, the decimal point is a small thing because it just happened to work out that way. There were other possible notations. Simon Stevin, the 16th century Belgian who really made decimal notation popular, noted decimal points by writing a little circle with the number of digits past the decimal above them. That is, he would write something like this for 3.1415:
As I say, he used circles, although in English it was modified to parenthesis — much easier to typeset, even today — in superscripts, that is, something like 31(1)4(2)1(3)5(4) instead. Vertical slashes were also sometimes used, thus, 3|1415. If it weren’t for the chance of confusing the decimal bar with the numeral 1 I’d probably vote for that one. John Napier, famous for pioneering logarithms, combined this with commas to produce a notation kind of like:
Semicolons seem, if Florian Cajori’s A History Of Mathematical Notations is still reliable, to have been surprisingly underused. 3;1415 seems like it would make the position of the decimal point quite clear and unmistakable without risking confusion with a numeral or requiring any complicated penmanship or typesetting. Cajori also gives examples of not just commas, but inverse commas (3’1415), and even little triangles. The best I can say is that if we were to go back and re-do the practice of representing concepts with symbols, knowing what we know now, we might be able to do a little better.
Scott Hilburn’s The Argyle Sweater (April 13) takes a break from anthropomorphic numbers jokes to give an anthropomorphic geography joke.
John Rose’s Barney Google and Snuffy Smith (April 14) puts forth as a silly idea writer’s block somehow spreading to mathematics. As I’ve often said, though, mathematics is a creative endeavor. To not know where to start a problem is something which can easily happen. But I grant the sort of problem likely to be assigned at Jughaid’s level probably would not require inspiration so much as skill at identifying what kind of calculation is supposed to be done and what information is needed for that calculation. Writer’s block would probably not come into play.
Art Sanson and Chip Sanson’s The Born Loser (April 14) presents lousy arithmetic as grounds for someone no longer being suitable for accounts receivable but a possible candidate for working on tax returns. The joke feels badly formed to me. Getting out of one’s taxes by creatively accounting for them is a matter of being cleverer than the auditor, or at least a matter of fooling oneself into thinking one’s cleverer than the auditor. This joke presents the idea of being stupider than the auditor, so it isn’t superficially plausible enough for me to buy into the joke.
Tim Lachowski’s Get A Life (April 14) name-checks sudoku in its joke about the arithmetic of taxes being so complicated people work crazily.
Mick Mastroianni and Mason Mastroianni’s Dogs of C Kennel (April 15) does a jokes about how it’s impressive to know a certain number of the digits of pi, although it’s tiresome to show them off. This is a good lesson about being a know-it-all in general. The part of the joke that isn’t obvious unless you happen to have memorized pi to just after the “653589” part — the next two digits are just where I drop off — is that Tucker, the dog reciting digits, has been doing on an extremely long time: the sequence “897232” doesn’t appear in the first 100,000 digits of pi. If he’s reciting two digits a second, then by the start of the second panel Tucker has been going on at least thirteen hours. Given that, I admire Will’s restraint. I got to liking this strip much better once I discovered that.
Bud Fisher’s Mutt and Jeff (April 15, and see above comments about rerun and writer status) tells a joke built around pairwise relationships. Normally we think of distance as having a property known as symmetry: if a is a certain distance from b, then b must be that same distance from a. Many pairwise relationships enjoy that symmetry: equality, for example, or the distance between points in space, or less obviously mathematical concepts like “is the same color as” or “is a sibling of”. But not every interesting relationship is symmetric: if a is some number less than b, it doesn’t follow that b is some number less than a.
And Jeff sets up his punch line around how “separation in space” and “separation in time” do seem to have a qualitative difference. Modern physics understands space and time as a joint entity, but there is something different in the way space works from the way time works. That there’s a compelling orientation of time is one of those ways. That said I just know that as a kid I would argue that it’s equally true to say it’s one week or it’s fifty-one weeks from Christmas to New Year’s. I would have been a difficult child in vaudeville-style jokes. | <urn:uuid:d6720da5-46b4-42e0-98a3-997e56f3835e> | CC-MAIN-2017-47 | https://nebusresearch.wordpress.com/2015/04/16/reading-the-comics-april-15-2015-tax-day-edition/ | 2017-11-24T05:43:01Z | s3://commoncrawl/crawl-data/CC-MAIN-2017-47/segments/1510934807089.35/warc/CC-MAIN-20171124051000-20171124071000-00278.warc.gz | en | 0.949685 | 1,780 | [
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IRB Application Guidelines
This section gives guidelines on how to create the following required documents:
o Research Protocol
o Informed Consent Form
o Institutional Consent Letter
o Additional Information
How to create a research protocol
The Research Protocol is a statement of the researcher's project design and a description of his/her responsibilities toward the human subjects involved in the research.
To ensure an effective review by the Institutional Review Board, a full description of the planned research must be submitted with the Application for IRB Review. A research protocol provides the reader with background information of the problem under study, including the study rationale, a detailed plan for conducting the research involving human research participants, and a discussion of the potential importance of the research.
The purpose of the study (research questions and / or study objectives) should be clearly and succinctly stated. In experimental designs, objectives may be stated as hypotheses to be tested.
2. Background and Rationale
Summarize and synthesize the available research (including published data) to provide justification for the study. Evaluate prior research for relevance to the research question under study. Describe the significance of the research including potential benefits for individual subjects or society at large.
The procedures should include the following:
a) Research Design
The research design should be identified and should be appropriate to answer the research question(s) under study. Describe the type of research proposed (e.g. experimental, correlational, survey, qualitative) and specific study design that will be used.
Describe the sampling approach to be used. Identify the procedures that will be used to recruit, screen, and follow study volunteers. Specifically define the study sample (number of subjects to be enrolled, characteristics of subjects to be included in and excluded from the research, and whether this will be a random or convenience sample).
Identify the variables of interest and study endpoints (where applicable). Justify measurement techniques selected. Provide information regarding the validity and reliability of selected measures.
d) Detailed study procedures
Methods for collecting data and for avoiding / minimizing subject risks should be included. Include a timeline for subject participation in the project. Identify how subject confidentiality will be safeguarded (plans for coding data and for securing written and electronic subject records). Indicate how long personal information will be stored once the study is completed. Methods will vary with the research approach used (qualitative, quantitative). The selected methods should be sufficiently described to justify the use of the approach for answering the defined research question. Methods should also be described in adequate detail so that IRB members may assess the potential study risks and benefits.
e) Internal Validity
Threats to internal / external validity should be considered. Describe measures that have been taken to avoid study bias.
f) Data Analysis
Specify the analytic techniques the researcher will use to answer the study questions. Indicate the statistical procedures (e.g. specific descriptive or inferential tests) that will be used and why the procedures are appropriate. For qualitative data, specify the proposed analytic approaches.
Include a reference list of literature cited to support the protocol statement.
How To Create an Informed Consent Form
The informed Consent Form must be a separate document from other documents. Except as provided in sections 4, 5 and 6 below, informed consent shall be documented by the use of a written consent form approved by the IRB and signed by the subject or the subjects' legally authorized representative. A copy shall be given to the person signing the form. The full informed consent form must include:
Content of the written consent form
statement that the activity involves research and a description of where the research activity will occur.
An explanation of the scope, aims, and purposes of he research, and the procedures to be followed (including identification of any treatments or procedures which are experimental) and the nature of the expected duration of the subjects' participation.
Description of any reasonably foreseeable benefits, if any, to the subjects or others that may result from the research.
A disclosure of appropriate alternative procedures or course of treatment (in instances where therapeutic procedures are involved), if any that might be advantageous to the subjects.
A statement describing the extent to which confidentiality or records identifying the subjects will be maintained except in unusual cases.
An offer to answer any questions the subjects may have about the research, the subject's rights or related matters, and the name of the person (together with address and telephone number) to whom the subjects may direct questions or must report an injury.
A Statement that participation is voluntary, that refusal to participate involves no penalty or loss of benefit to which the subjects are otherwise entitled, and that the subjects may discontinue participation at any time without penalty or loss to which the subjects are otherwise entitled if they had completed their participation in the research.
For research which may involve more than minimal risk of injury the subject should be informed of the following statement which must appear in the consent form: (to be modified for off-campus research). "In the unlikely event of injury resulting form this research, Andrews University is not able to offer financial compensation nor to absorb the costs of medical treatment. However, assistance will be provided to research subjects in obtaining emergency treatment and professional services that are available to the community generally at nearby facilities. My signature below acknowledges my consent to voluntarily participate in this research project. Such participation does not release the investigator(s), sponsor(s) or granting agency(ies) from their professional and ethical responsibility to me."
A space for the dated signatures of the subject, the principal investigator, and a witness. In the case of a minor (the child must also sign if seven years of age or older) or a person unable to sign, a second authorizing signature is required from the parent, guardian, or other responsible person. The relationship must be specified.
In addition there is need to pay special attention to the following two definitions in your Informed Consent, since lack of coercion and confidentiality are required for approval:
Coercion means to compel or force someone to participate in or perform an action that would not ordinarily be done of the individual's own free choice. Coercion may be present when recruiting subjects for research. Participation should be free and voluntary, with no overriding statements. The following are ways that coercion can be introduced: The researcher is the supervisor or pastor of the participants. Telling subjects or their parents (when children are involved) how much they will be helping the investigator by participating in research can be interpreted as coercive. Mentioning a relationship that exists between the researcher and the potential subjects may be coercive. Subjects may feel obligated to participate because they know or have seen the researcher at various times. In cases of infants and children, mentioning that the researcher cares for or has cared for the child puts parents in a very awkward and unfair position. Face-to-face recruitment has the potential to be coercive. It is difficult for individuals to say no to someone who is directly in front of them and talking about his or her research. Inflection, tone of voice, and nonverbal cues can inadvertently slip into the recruitment process without the researcher's awareness. Coercion can be reduced if an impartial third party presents the request for participation. Subjects should be protected from coercion. If subjects are not protected, the IRB application must include an explanation of why coercion is necessary as well as any possible repercussions of the coercion. The methods to be used for coercing subjects must be detailed in the research proposal. A plan for informing subjects at the end of the research of how and why they were coerced must be fully explained (see Debriefing). Potential physical and/or psychological risks that may be incurred by subjects due to the coercion must be identified, and procedures for addressing the risks must be established as part of the debriefing procedures.
Confidentiality refers to protection of subjects' privacy so that information collected about them, as part of the research process, is not disclosed. Information may be revealed in group form, or as individual examples, but not in a way that an individual may be identified. If the investigator collects information on subjects over a period of time, such as in test-retest reliability or in
pretest-posttest study designs, there must be a mechanism to relate various data to the same subject. This may be done by using codes or identifiers (e.g., subject ID numbers) on both sets of data that only the researcher can trace to a master name-number list. Because names and numbers can be related, this list must be kept confidential by storing it in a private and secure location, such as a locked file cabinet. If data are recorded in cases where the researcher personally knows subjects, it must be acknowledged that the researcher knows the subjects personally, and the data must be treated confidentially, because anonymity is not possible. The data must be collected in such a way that the identity is not recorded. All data should be stored in a way that the person is not identified when the identity is not crucial for the research objectives. In other words, the IRB will require that data be collected in the least intrusive and most confidential way to serve the purpose of the research. In a focus group situation, it must be acknowledged that there is a lack of confidentiality due to the group situation. The consequences of this lack of confidentiality must be outlined. It is important to acknowledge
It is important to acknowledge that subjects may waive the right of confidentiality. This may occur, for example, when a subject specifically requests to be quoted. In the United States, all confidential data must be stored by the researcher for 3 years. In Canada, data must be stored for 6 years.
Consider also that ethical research requires that the researcher is qualified to do the research they are proposing.
Format of the Written Consent Form
The consent form should clearly identify the relationship of the researcher to Andrews University. The name of Andrews University should appear centered at the top of the consent form together with the name of the department with which the researcher is affiliated. In cases where an anonymously returned questionnaire substitutes as a form of implied consent, the cover letter accompanying the questionnaire should clearly identify how the research is connected with Andrews University and one of its academic departments.
The consent from should clearly indicate the name, address, and phone number of the investigator and an advisor or impartial third party whom the research subject may contact for additional information if desired.
Places for the dated signatures of the subject (and/or parent/guardian, if applicable), investigator, and witness should be included at the bottom of the consent form.
Retention of the Signed Informed Consent Form
A copy of the Informed Consent Form should be returned to the subject or the person legally appointed to sign the Informed Consent Form to retain for his/her review.
The responsibility for retaining signed copies of the Informed Consent Form lies with the principal investigator(s). These Informed Consent Forms should be kept in a secure depository along with the researcher's other records for a reasonable amount of time (not normally to exceed three years).
Use of Alternate and/or Simplified Consent Forms
Certain situations may justify the use of alternate and/or simplified consent forms. However, in all cases the investigator must demonstrate how the anonymity or confidentiality of the subject and his/her voluntary participation in the project will be assured and maintained.
Oral Instructions Read to a Group. In the case of no risk or minimal risk research where instructions are read to a group of subjects (e.g. a questionnaire passed out in a classroom setting, with prior written authorization of the instructor), a short form to document the oral instructions presented to the subjects may be used. A witness who heard the oral instructions read to the group must co-sign the short form along with the researcher. A written copy of the oral instructions that are to be read to the group must be submitted with the protocol. The items listed in Section 1 above should be included in the oral instructions.
Research using surveys or questionnaires and dealing with sensitive areas of the respondent's own behavior (illegal conduct, drug/alcohol use, sexual behavior, etc. See Appendix A, Exempt Review, item 4) require special consideration. Although the purpose and use of surveys or questionnaires in such research may be explained in a classroom setting (with prior documented permission of the instructor(s) involved), requesting respondents to actually complete survey instruments in the classroom setting is not recommended. Alternative methods of collecting forms completed at the discretion of the respondent and which thus insure the respondent's anonymity should be employed.
Anonymous Surveys or Questionnaires. In the case of risk or minimal risk research involving the use of surveys or questionnaires which are distributed individually and returned anonymously, the cover letter explaining the purposes and procedures of the research project may substitute for the consent form. Such a cover letter must be submitted with the protocol and should contain reference to the items mentioned in section 1 above. It should state in the cover letter as well as on the survey form itself that the return of the survey or questionnaire serves as a form of implied consent.
Simplified Oral Interviews. Investigators conducting simple oral interviews, the content of which qualifies as exempt from review, may submit an alternate form of written documentation in place of an informed consent form. Such documentation should describe how the interviewer will explain his/her research to the interviewee and how the researcher is prepared to insure the interviewee's confidentiality and his/her right to refuse participation in the interview.
In all cases, the researcher is responsible for the filing of all proof of compliance with the above procedures and to keep them for a period of three years
Waiving of Signed Consent Documentation
The IRB may waive the requirement for the investigator to obtain a signed consent form for some or all subjects if it finds that either of the following conditions exists:
The only record linking the subject and the research would be the consent document and the principal risk would be potential harm resulting from a breach of confidentiality. Each subject will be asked whether he/she wants documentation linking the subject with the research, and the subject's wishes will govern.
The research presents no more than minimal risk of harm to subjects and involves no procedures for which written consent is normally required outside of the research context.
Waiving the Consent Process
The IRB may under certain special circumstances approve a consent procedure which does not include or which alters some or all of the elements motioned above or may waive the requirement to obtain consent provided the Board verifies and documents each of the following items:
The research involves no more than minimal risk to the subjects
The waiver or alteration of consent will not adversely affect the rights and welfare of the subjects.
The research could not practicably be carried out without the waiver or alteration.
When ever appropriate, the subjects will be provided with additional pertinent information after participation.
Consent form from Attending Physician and/or Other Health Care Professionals
In situations where an individual is currently being treated/evaluated by a physician and/or other health care professional for a condition related to the objective of the research study, the researcher is required to obtain the consent of the physician and/or health care professional prior to involving such research subjects in the study.
How To Create An Institutional Consent Letter
It should be written on an Institution's/Company's letterhead;
It should mention the researcher/investigator by name;
It should mention the title of the study for which institutional consent is being given;
It should be dated;
It should include the name and the title/office of the individual within the institution providing the consent;
It should be signed by an authority of the institution;
It should be addressed to:
Institutional Review Board
4150 Administrative Drive, Room 322
Berrien Springs, MI 49104-0355
Or faxed to attention IRB : (269) 471-6543
E-mail Letters: Letters may be sent as scanned email attachments to [email protected].
How to Create a Survey (Questionnaire)
Your Survey Instrument must be a separate document from your other documents.
If your research falls under the category: Exempt from Full Review, your research might be of a nature that allows you to opt to go with an Informed Consent Letter (please see layout samples). If you do opt to do this, please be sure that you include the following statement near the beginning of your survey instrument, before the fist application:
I have (received/read/had the Informed Consent Letter read to me)* and recognize that by completing and returning this survey, that I am giving my informed consent to participate. *Please adjust the wording in parenthesis to meet your needs.
If your research involves face to face interviews, and your questions are open-ended, please provide the head-off questions and a brief statement assuring the Institutional Review Board that any further questions arising from the initial questions will remain within the framework of the research approval that you seek.
Otherwise, your whole survey instrument must reflect in both content and layout the survey that will be received by your human subjects.
Survey questions need to be clear and well-constructed and should result in answers that will give you reliable results, especially in instances where there is a diversity of respondents.
Please be sure that spelling and grammar reflect high standard that honors both you and Andrews University.
If the survey is conducted in a language other than English, please provide the IRB with a copy of the alternate language (original) and an accurate English translation. This also applies to the Informed Consent Form/letter.
go to top | <urn:uuid:1ba1b20e-21fb-41d0-b889-7128c3ad7abb> | CC-MAIN-2017-47 | https://www.andrews.edu/services/research/research_compliance/institutional_review/guidelines.html | 2017-11-24T05:35:50Z | s3://commoncrawl/crawl-data/CC-MAIN-2017-47/segments/1510934807089.35/warc/CC-MAIN-20171124051000-20171124071000-00278.warc.gz | en | 0.922798 | 3,601 | [
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Parish Council Meeting Held at 7.15pm on Tuesday 15th September 2015 at Eastnor Cricket Club
Denis Brandt (DB), Robin Smith (RS), Christine Stallard Daniels (CSD), Shane Howells (SH), Shirley Roberts (SR), Caroline Layton (CL) and Imogen Puddock (IP).
Emma Holton (EM) CSD acted as clerk as clerk absent at funeral
To consider the acceptance of apologies for absence from councillors
No apologies received.
To receive declarations of interests and written requests for dispensations
Opportunity for Speakers
EH gave update regarding library closure consultation, budget cuts over
the next 4 years (£42 million to be saved) 75% of budget goes on adult
and social care, fracking in the county.
To consider the adoption of the minutes of meeting 7th July 2015
Were considered and signed as a true and accurate record AGREED.
Co-option of Eastnor Councillor Vacancy
Matthew Collis Long has decided not to stand as parish councillor due to work
commitments. Therefore Eastnor is still short of one councillor.
Eastnor councillors will ask around.
Cheques presented for Signature:
Clerk Salary for September and October
CN000432 Clerk Expenses
CN000435 Eastnor Cricket Club Room Hire
Halc Training Course
Cheques AGREED by all and signed off.
Annual Return signed off by External Auditor.
Audit requirements are changing next year. Still waiting for confirmation but have
been advised by Halc internal auditor may have to check accounts more frequently
than once a year and have to pass certain criteria in order to act as Interal Auditor.
Now Matthew has stood down as councillor the parish council need a replacement
cheque signatory. Currently Denis, Robin and Caroline are signatories, one more is needed.
Update on Parish Council Website and Transparency Code
Parish Council website at eastnordonningtonparishcouncil.co.uk
Training to be provided soon to the clerk on using the website and loading
information. Councillors were asked to volunteer if happy to participate in training.
There were no volunteers.
Clerk attended Halc website course. Parish Council can apply for grant to cover
costs of website, training, printer and laptop.
Form provided for review by parish council and to be signed by Chair. Chair
to complete form with clerk AGREED.
To consider planning applications:
152504 – Fell Trees – Eastnor Estate – No objections.
152405 – Fell Trees – The Old Post Office, Eastnor – No objections.
Road Safety Issues with Eastnor and Donnington
EH to e-mail link to clerk to pass on to councillors to report problems on to
Belfour Beatty website.
Leaflets distributed regarding registering to vote.
Date of next meeting and Agenda
Tuesday 17th November – Donnington, Brooms Green & Ryton Memorial Hall
Parish Precept & Budget for next financial year
Disposal of old laptop
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Kubo and the Two Strings (2016): Written by Marc Haimes and Chris Butler, directed by Travis Knight. Starring: Art Parkinson, Charlize Theron, Ralph Fiennes, Matthew McConaughey, and Rooney Mara. Running Time: 102 minutes.
If you must blink, do it now. For if you look away for even a second, this review will fail, and it’s writer will surely die. Or at least be condemned to watching lesser animated fare like Sing or The Secret Life of Pets for the rest of eternity. *shudders*
This threat is not terribly dissimilar to the one facing our hero, Kubo (Art Parkinson), as he seeks to discover the legacy of his late father, the legendary samurai Hanzo. Possessing the ability to use his magical shamisen to manipulate objects like leaves and pieces of paper however he wishes, Kubo is at first wholly ignorant of why he and his mother live alone in a seaside cave, why he is strictly forbidden from leaving the cave at night, and why he only has one eye. He finds out in terrible fashion one night when he stays in the local village just a bit too long, and his mother’s twin sisters (both expertly voiced by Rooney Mara) descend from the moon to kill him and his mother and steal his remaining eye.
His mother uses the last of her magic powers (she appears to have once a powerful sorcerous, but grief over the loss of her husband slowly dimmed her strength over the years), to send Kubo away under the protection of a monkey talisman brought to life. Kubo and the monkey (Charlize Theron) are soon joined by an anthropomorphic beetle (Matthew McConaughey) claiming to have been trained by Hanzo himself. They set out to find the legendary three great treasures that offer Kubo’s only defense against the powerful magic of the sisters and their father, the Moon King.
It’s a perfect example of the classic Hero’s Journey we’ve seen a thousand times before, yet done so point-perfect and with such obvious vision that it rises above most of its competitors into something unique and special. This movie was in production for almost a decade, and it shows, as its lavish and detailed visual design left me gaping in every other scene. Everything from the way hair and clothing moves to how waves rise and fall looks so realistic you have to keep reminding yourself you’re watching clay figures that were photographed a shot at a time.
This is so much more than a lights show though. The voice acting breathes beautifully animated life into the characters, and when you least expect it to, the movie uses its story to turn to themes of forgiveness, love, and family, and the importance of building community with those around you. There are story quibbles to be had- later reveals about the background of Monkey and Beetle raise more questions than they answer- but these are minor distractions from what is otherwise one of the best movies, animated or otherwise, to come out this year.
I still find it supremely disappointing that Laika has never had quite the box-office success (nor the awards success) that the other major animations studios in America have had, because they have firmly established themselves as the new Pixar of the US animation scene, created brilliant, original, and cutting-edge works that push the boundaries of stop-motion animation much like how Pixar has expanded the possibilities of computer animation. By all rights this and ParaNorman both deserved much better success than they had (this one just barely broke even at the box office).
That said, Kubo is still such a masterful piece of work, I am confident it will still find its audience and will remain and enduring work for years to come. Like its score and the magical, reality-altering music of Kubo himself, it rises and soars on its own vision to heights most movies can only dream of reaching. | <urn:uuid:a1ed2012-e098-47c3-9215-9aea2ffac3ae> | CC-MAIN-2017-47 | http://francnoir.blogspot.com/2016/12/review-kubo-and-two-strings.html | 2017-11-18T00:46:21Z | s3://commoncrawl/crawl-data/CC-MAIN-2017-47/segments/1510934804125.49/warc/CC-MAIN-20171118002717-20171118022717-00478.warc.gz | en | 0.96716 | 818 | [
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Lot 8 Mount Allen Road Kapunda SA 5373
LIVE THE DREAM!!!Lot 8 Mount Allen Road, Kapunda
Looking for the perfect rural location to build your dream home (STCC) and create the ideal rural lifestyle to raise a growing family or relax in retirement or yearning for a quiet self sufficient life on 11 fabulous acres with stunning 270degree views across open paddocks and rolling hills? No close neighbours in a tightly held and much sort after Allendale North community. Only a few minutes north of the historic township of Kapunda with the convenience of the school bus a short stroll away.
Room for the motorbikes, ponies and the menagerie of farm animals. Design your home to capture the views with room enough for the biggest must have shed, veggie garden, fruit orchard, tennis court and swimming pool if desired. Significant and costly excavation has been done to create the ideal base or site for a home or shedding. Mains water connected and water meter installed. The options are plentiful.
local school bus service availableWith the local watering hole, better known as the Wheatsheaf Pub just up the road. The perfect spot to wet the whistle," wine down"or meet friends.
Contact Julia Smith 0427 605 059
Ray White Barossa Valley
- No inspections scheduled
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Real Estate For Sale in Kapunda, SA 5373 priced between $110,000 and $200,000 | <urn:uuid:12d640d5-2b2b-4022-8caa-cb92bb943e50> | CC-MAIN-2017-47 | https://www.thehomepage.com.au/property/residential/lot-8-mount-allen-road-kapunda/1795216 | 2017-11-18T00:59:59Z | s3://commoncrawl/crawl-data/CC-MAIN-2017-47/segments/1510934804125.49/warc/CC-MAIN-20171118002717-20171118022717-00478.warc.gz | en | 0.908065 | 331 | [
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This Sterling is finished in our Original Drayton Green, the colour we chose for the first unit we produced. It’s darker than the British Racing Green, and it gives a very vintage look. The chassis is powder coated in Pitch Black. The logo is hand silk-screened onto to the tank in Vintage Gold and we hand paint the pinstriping in Vintage Gold and Ivory White on the coachwork. The leather is our top “Testa di Moro” vintage finish, which matches the brown grips.
The springs, valve cover and headlight bezel are nickel-plated for a deep, complex finish that is more subtle than chrome, as well as period appropriate. The “Herring can” headlight gives an even more accentuated 1920s look. Polished downpipe, a black silencer and Avon Speedmaster tyres complete this version. | <urn:uuid:e25b6de4-ca8c-4d1c-a5cc-2aac854eb9ea> | CC-MAIN-2017-47 | http://theblackdouglas.com/model/original-drayton/ | 2017-11-18T19:24:00Z | s3://commoncrawl/crawl-data/CC-MAIN-2017-47/segments/1510934805023.14/warc/CC-MAIN-20171118190229-20171118210229-00478.warc.gz | en | 0.931108 | 182 | [
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IPhone IOS 10 Wallpaper
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words for easier search on this site. It has 459 views and 0 downloads by users. | <urn:uuid:b6002199-ed51-47ee-9567-ad2f2ae944a8> | CC-MAIN-2017-47 | http://www.wpaperhd.com/wallpaper/hi-tech/iphone-ios-10/WpwW | 2017-11-18T19:17:29Z | s3://commoncrawl/crawl-data/CC-MAIN-2017-47/segments/1510934805023.14/warc/CC-MAIN-20171118190229-20171118210229-00478.warc.gz | en | 0.8863 | 109 | [
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Another car making its U.S. debut in the L.A. show is the Mazda2. It is already popular with motorists around the world and has been named "Car of The Year" in no less than 20 countries since it first appeared three years ago. The Mazda2 has undergone a facelift for the U.S. and should get better than 40 mpg. It is expected to start selling in the North American subcompact market in the second half of 2010.
Next Mitsubishi MiEV | <urn:uuid:ecb5e2ca-1b30-4bc8-9213-fbed0db920d8> | CC-MAIN-2017-47 | http://content.time.com/time/specials/packages/article/0,28804,1945790_1945788_1945776,00.html | 2017-11-19T15:26:00Z | s3://commoncrawl/crawl-data/CC-MAIN-2017-47/segments/1510934805649.7/warc/CC-MAIN-20171119134146-20171119154146-00478.warc.gz | en | 0.975414 | 106 | [
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Today’s stocking stuffer is A Christmas Shift by my good friend Angelina Windsor. It’s a hot one!! Check it out.
A Christmas Shift
Angelina J. Windsor
Aspiring actress Lacy Kennedy arrives in Spirit Lakes, Alaska, a few days before Christmas to spend the holidays with her brother and his family. She’s expecting snow and trees and home cooking but finds instead something she could never have imagined…and doesn’t want.
Just who does tall, broad and sinfully handsome Jackson Stone think he is? Oh, him wanting to screw her is fine. She’s panting for him too. What’s a no-no is all that comes with it. Because they’re wolf shifters and—Lacy suspects—life mates, so once they bond, that’ll be it. It’ll be the marking and the binding and poor Lacy doomed to a life in the snowy north, a million miles from the bright lights of La-La Land and her dreams.
But maybe, just maybe, Christmas is a time for new dreams.
Reader Advisory: This story has graphic sexual language and scenes—no closed bedroom doors (or other rooms) here!
An adult paranormal romance from Ellora’s Cave
And now for an excerpt!!
“Oh my god! What were you doing just standing there?” A man looms over me. Not just any man, but “The Man” everyone talks about when they’re fantasizing about their dream lover. I might have been more excited about this burning hunk of love if I weren’t sprawled like a cat enjoying a patch of sunshine at his extra-large feet. His prominent package appearing to match his ginormous shoe size is at the perfect vantage point and I take a full second to enjoy it before I’m hauled to my feet. What is that delectable fragrance? I breathe in deeply and feel all my senses reel in recognition. No. No. Nooooo. Goddamn it all to hell! What’s he doing in Spirit Lakes, Alaska? No fuckin’ way. I’m not going to be bound by convention. Just because there is supposed to be one mate for everyone and you know that as soon as you meet them doesn’t mean I have to follow the shitty rule and fuck up my whole life. I am an LA kind of girl. Not a life mate for a mountain man. No way.
Still December 21st
“Well, darlin’, you didn’t have to fall at my feet for me to notice the likes of you.”
“That’s two strikes, buddy. One more and you’re out,” I whisper harshly as I smooth down my dress, giving him a full-on TV glare. I don’t want anyone else to notice this dismal entrance. But I realize with dismay that’s an improbability as the crowd behind us is growing quiet. I sense their full rapt attention as my eyeballs appear to be glued to him of their own accord. I gulp nervously. He’s too tall, too handsome and too full of himself.
His mouth curves upward into a smirk and my thoughts turn to kissing those fine, full lips. I try not to breathe but his overwhelming macho-man fragrance turns me on whether I want it to or not. Think John Travolta’s character in the movie Michael. This is great. This is really, really bad.
“See anything you like, beautiful?” he asks, his eyebrows rising as he gets his first good full-on whiff of my lush fragrance. Hell, even I can smell my arousal. My nipples are tightening, my clit is pulsating and my mind has gone to mush.
“Not really,” I deadpan as I bite hard into my bottom lip to keep a more honest answer at bay. My acting ability is all that stands between me and complete disaster.
His eyes bore into mine as if trying to see into my soul and I swallow hard. This is soooo not supposed to happen. I see indecision in his eyes for a full nanosecond. I relish taking down his alpha-male confidence a notch while my girly side goes into hyperdrive. What the hell are you thinking? Jump his bones—now! You know the true test.
He holds out his hand and I reluctantly take it. An electrifying shock almost makes me drop it but I hold on long enough to give it a quick shake. Then drop it like the ticking bomb it is.
Find out more about Angelina Windsor here: | <urn:uuid:fd7996e0-64c0-4e9e-8a33-2c26110d50f9> | CC-MAIN-2017-47 | https://wendizwaduk.wordpress.com/2015/12/14/stocking-stuffers-a-christmas-shift-by-angelina-windsor/ | 2017-11-19T14:11:21Z | s3://commoncrawl/crawl-data/CC-MAIN-2017-47/segments/1510934805649.7/warc/CC-MAIN-20171119134146-20171119154146-00478.warc.gz | en | 0.965291 | 992 | [
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Panopto offers many functions, which we breakup into three workflows: Using Panopto with Blackboard, Using Panopto Separately From Blackboard, and Panopto for Students. This Roadmap is designed to help you determine which of Panopto’s workflows will suit you best and then link you to the appropriate posts that will give you the detailed instructions to start working with Panopto.
What is Panopto?
Panopto is the University of Pittsburgh’s secure video hosting and lecture capture recording platform. If you wish to record a lecture or provide a narration for a PowerPoint presentation or do a simple web cam recording to supplement your online course material, or if you have a video file that you wish to provide to your students and do not want to post it to a public site such as Youtube, Panopto can be used to record that video presentation, as well as deliver this video to your desired audience.
Panopto video presentations can be delivered through Blackboard CourseWeb, However, you are not obliged to use this Blackboard integration. Video presentations hosted on Panopto can be shared independently of Blackboard, either through viewer links, an embedded video, a podcast or even a SCORM package that can be emailed or posted on other websites.
Regardless of your choice above, Panopto video presentations are hosted on Pitt’s video website called My Pitt Video. You can directly upload video files that you have captured or downloaded on your computer or an SD card. However, Panopto offers a video creation tool as well, which can capture and combine web cam video, a PowerPoint presentation, and/or a screen recording. You can use any combination of these video streams using the Panopto Focus Recorder to capture and upload these recordings to the Panopto website.
Finally, before you begin your recording, please review this list of Guidelines for Creating Instructional Recordings for some general tips for recording the best videos for your academic environment.
If you have a Blackboard course, and want to deliver your videos to your enrolled students while restricting your videos to your students (not allowing people outside your course to view your videos), then you’ll want to use Panopto with Blackboard.
- The first thing you will need to do is connect your Blackboard Course with a folder in Panopto. This will involve creating a Panopto Tool Link in your course. To do this, you can follow the instructions on our Getting Started With Panopto page.
- Once you have connected your Blackboard course with a Panopto folder, you can begin Creating Videos in Panopto. This tutorial will go over the different methods of adding video content to Panopto, from using the Panopto Focus Recorder to directly uploading a video to creating additional sub-folders. Be sure to do this using the “Create” button in your Panopto Tool Link in your Blackboard course, or on the “Create” button on the Panopto website in the correct folder.
- Once you’ve created your videos, you can either manually add a Panopto video to your Blackboard course (recommended) or use the Panopto Tool Link as a video repository for your students to use (less preferable).
If you do not want to rely on Blackboard to deliver your Panopto video presentations, you can simply use the Panopto website as a hosting platform.
- We recommend creating a Sub-Folder within your personal Panopto folder for this purpose.
- If you have created a sub-folder, this will allow you to manually manage the Share settings. If you did not, you can adjust the share settings on your personal folder as well. You can set the folder to private, and restrict it to the viewers you want, or set the folder to a more accessible status to make it available to all Pitt users, or alternatively, to any user on the internet.
- Once you’ve set up your folders, you can begin Creating Videos in Panopto.
- Once you’ve created your videos, you can deliver your videos in a variety of ways. To learn more about how to deliver your videos, find your video’s Video Settings, and then navigate to Video Outputs. Again, be sure that your intended audience aligns with your Share settings, or your audience will not be able to view your videos.
Here’s how to allow your students to upload videos to Panopto.
- First, you must set up a folder for them to upload to. There are two ways to do this.
- If you wish for your students to upload into a shared space where they may collaborate and modify each others’ videos, then you can Create a Sub-Folder within your Provisioned Course Folder –OR- your personal Panopto folder. Then you must adjust the Share settings for this folder to include each of those students as Creators in that folder. If you have a large number of students, it is possible to add multiple users to the share settings at once, and you can download your student directory from either Blackboard or from PeopleSoft and copy/paste the student usernames all at once.
- If you wish for your students to upload their videos to you privately, where they cannot view or modify each other’s work, you must create a Drop Box folder. This can only be done using a folder connected to a Blackboard course, and this will apply only to students in your Blackboard course.
- Once you’ve set your students up as Creators, they should each have access to this folder, as well as a blue “Create” button when they log into the Panopto website through their “My Pitt Video Login.” Please direct your students to the Student Knowledge Base, and the Panopto Workflow for Students page to assist them with uploading content to Panopto.
Once you’ve created, uploaded and distributed your videos, or if you anticipate making video presentations a regular part of your course, and want to take the time to make your videos the best they can be, there are a few additional steps you can take.
- Guidelines for Creating Instructional Recordings – Again, for some general tips for creating the best videos for your academic environment.
- The Right Hardware for Panopto – Improve the quality of your video recordings by using the best tools available.
- Student Release Form – If you have any concerns about student recordings being placed online, please make use of this release form.
- Edit Your Videos – This will allow you to make adjustments and trim any bits of dead space in your presentations.
- Caption Your Videos – Make your videos accessible to viewers with special needs.
- Frequently Asked Questions – If you have any questions about using Panopto, you can find some helpful answers here.
- The CSSD Help Desk is available 24 hours per day, seven days a week, 365 days each year at 412-624-HELP (4357). They are able to solve many common Panopto and Blackboard issues.
- The Educational Technology Services (ETS) team also supports Panopto, as well as Blackboard and the operations of the Educational Technology Center (ETC) technology training programs for faculty and staff. ETS provides individual and departmental consulting and training opportunities for faculty and staff.
Location: Alumni Hall, Room B23
Business Hours: 8:30-5:00 Monday-Friday
Contact Information: 412-648-2832 / [email protected]
- ETS regularly offers workshops for instructors and staff on many aspects of Blackboard and other instructional technologies, including Panopto, from introductory sessions to more advanced topics.
- The ETS News + Notes electronic newsletter is sent out twice a month, and contains important announcements, handy tech tips, and timely links to pertinent Knowledge Base articles. | <urn:uuid:6efad1b7-7ebb-4f73-90e6-c99975a1e167> | CC-MAIN-2017-47 | https://www.etskb-fac.cidde.pitt.edu/panopto/getting-the-most-out-of-panopto/ | 2017-11-19T13:57:54Z | s3://commoncrawl/crawl-data/CC-MAIN-2017-47/segments/1510934805649.7/warc/CC-MAIN-20171119134146-20171119154146-00478.warc.gz | en | 0.907101 | 1,658 | [
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Saxion University of Applied Sciences offers their own bachelor scholarships for talented (non-European) students. Full details:
School (Institution / Country): Saxion University of Applied Sciences | Netherlands
Level: Undergraduate Degree.
Field(s): Applicant must be in or starting the admission procedure to one of Saxion’s International Bachelor Programmes:
Deadline: 1st May 2013.
Funded By: Saxion University of Applied Sciences
Beneficiaries( Target Group): Scholarship is Open for the student with a nationality from outside the EU/EEA.
Benefits( Value/ Inclusions):
‣ No. Awards: Not Specified.
‣ Scholarships Duration: Scholarships are awarded for one academic year.
‣ Scholarship Covers: It covers the tuition fee, with exception of the governmental registration fee which is approximately € 1,835. (for 2013/2014).
‣ Saxion Uni. of Applied Sciences offers their own bachelor scholarships for talented (non-European) students.
‣ Saxion has offered Bachelor & Master programmes and Short Courses in English for more than ten years now and our programmes have proven to be extremely popular with students from around the world.
‣ At present students taking our programmes come from over 55 countries as diverse as Indonesia, Germany, India, Norway, China, Taiwan, Russia, Brazil, Rwanda, Japan, Bulgaria, Poland, Lithuania, Vietnam, Romania, and Turkey, to mention but a few.
Candidates must meet the following criteria to avail Saxion University Top Talent Scholarship (STTS).
‣ must have outstanding study results, with an average mark of 75% or higher ;
‣ must have an IELTS score of 6.5 or higher,
‣ must be in or starting the admission procedure to one of Saxion’s International Bachelor Programmes as a new student in the bachelor programme,;
‣ must be a student with a nationality from outside the EU/EEA
‣ Scholarships are only awarded for programmes of at least 6-month duration,
‣ Scholarships are awarded for one academic year.
To apply for a Saxion Top Talent Scholarship, you have to meet the following criteria:
‣ IELTS score 6.5 or higher, -Outstanding study results, you have an average mark of 75% or higher,
‣ must be in or starting the admission procedure to one of Saxion’s International Bachelor programmes as a new student
Scholarships Application Method:
‣ To apply for a Saxion Top Talent Scholarship, please fill in this form , and send it back to us, preferably together with your application. If you already have sent in your application you can also send it separately to:
Saxion University of Applied Sciences Attn: International Office - Scholarships P.O. Box 501 7400 AM Deventer
The Netherlands Fax nr. : + 31 570 603628 E-mail: [email protected] (only scanned document with authentic signature!)
‣ Scholarship Applications are due by 1st May 2013 for enrolment in September 2013.
‣ It is important to read the application guideline , and visit the official website (link found below) for detailed information on how to apply for this scholarship.
‣ Application forms and guidelines can be downloaded from the official website
You may also like | <urn:uuid:ed7e7b12-2518-4f24-8b26-54ee9b1edaee> | CC-MAIN-2017-47 | http://www.scholarshipsbar.com/saxion-university.html | 2017-11-20T09:41:24Z | s3://commoncrawl/crawl-data/CC-MAIN-2017-47/segments/1510934805977.0/warc/CC-MAIN-20171120090419-20171120110419-00478.warc.gz | en | 0.913214 | 706 | [
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A high-availability system must have the ability to identify and correct errors, exceptions and failures in a timely and reliable manner to meet challenging service level objectives. The Teradata database and the utilities and components (used to both load and access data) provide capabilities to implement reliable error and exception handling functionality. These capabilities combined with a well designed high availability architecture allow a Teradata Active Enterprise Intelligence (AEI) system to meet the service level objectives required to support mission critical business processes.
This series of articles focuses on the error handling functionality and restart capabilities of the Teradata database and the Teradata data load utilities.
Examples of error and restart handling for the following utilities are included in this series:
In most cases a load utility job for each of these utilities can be re-started after a database failure and reset, a load process error or failure or a load utility client platform failure after the underlying problem has been corrected.
An Active Enterprise Intelligence Ecosystem consists of not only the Teradata database subsystem but all of the surrounding subsystems that require service from the database subsystem and provide services to the database subsystem. The following diagram illustrates the major subsystems in an AEI Ecosystem.
Each subsystem must have error and failure handling capabilities. This series covers the capabilities and approaches to handle database, data integration and application integration errors and failures. This series will focus on handling errors, exceptions and failures in a Data Integration subsystem that is implemented with Teradata load utilitities.
A system can encounter multiple types of errors and failures. Error and failure handling processes must execute the following steps:
The first step is to identify that an error or failure has occurred. In most cases a failure of the database will be detected by either a data load client or a data access client during a database operation. In this case the error must be classified and reported by the database client. In other cases the client may fail during a database operation. In each case the error must be classified.
Errors in the database or a database client can be classified as:
The ability to restart Teradata load utility operations that are interrupted by an error or exception is a key feature of these utilities. Care must be taken to identify the error properly to determine whether a restart is possible and to configure the utility scripts properly for restarts.
It is critical that errors and exceptions be identified and classified. This is necessary to determine whether the problem can be isolated and the system returned to service automatically using Teradata high availability features or if the error requires intervention. In the case of intervention the type of error will determine whether the intervention is by a DBA, programmer or Teradata support personnel.
Teradata load utilities have robust error handling and restart capabilities. This allows a database operation that is interrupted by a system error or by excessive data errors to be restarted and not have to be completely re-executed. A data integration operation may be interrupted by database errors, script errors, data errors or failures of a client process or platform. The Teradata load utilities provide the capability to restart a load operation in all of these cases.
Data integration operations are usually implemented as jobs that contain a series of steps. The steps may be defined in a single Teradata load utility script or as series of steps that execute load utilities or SQL scripts. In each case the job should be step restartable. That means that after an error the job should be able to be restarted at the point or step that was executing when the error occurred. In addition, each step should be able to be restarted or re-run. A restarted step will not re-execute the database interactions that occurred before the error. A step that is re-run will re-execute all database interactions in that step and continue with succeeding steps.
Jobs can be defined and executed using job scheduling and control systems (eg. Control-M, Tivoli, AutoSys), data integration systems (eg. Informatica, Oracle ODI, DataStage), or with scripts (eg. shell scripts, perl). In each case the job scheduling and control system should allow a job to be started at a step that was executing when an error occurred and it should allow a step to either be restarted or re-run.
Teradata utilities allow steps defined within the utility scripts to start at the step where an error occurred or to be rerun completely. They also allow a job interrupted by a database reset to continue automatically once the database is back in service.
The utilities allow checkpoints to be established to prevent the re-execution of database operations that have completed before an error caused an interruption.
Each of the load utilities allows parameters to be set that control:
All of the Teradata load utilities provide a return or completion code to the operation system or program that executed the utility. All job control definitions should check the return codes and take the appropriate action based on the return code. The Teradata utilities return the following completion codes:
In each case the specific database error must be found in the output log of the load utility to determine the next course of action (fix, re-start, re-run). A job control system can parse the output logs to determine and classify the database errors (beyond the scope of this series of articles). In all cases the underlying error must be identified, classified, fixed and then the job restarted at the appropriate step.
In the following series of articles we will describe how to use the features of Teradata load utilities to handle database and client errors and failures. | <urn:uuid:ed0c645b-7caa-482e-ab81-4814cfb4bee2> | CC-MAIN-2017-47 | http://community.teradata.com/t5/Tools/Teradata-Utility-Error-Handling/td-p/56202 | 2017-11-21T04:26:16Z | s3://commoncrawl/crawl-data/CC-MAIN-2017-47/segments/1510934806316.80/warc/CC-MAIN-20171121040104-20171121060104-00478.warc.gz | en | 0.876024 | 1,125 | [
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Okay, this is awesome. Lifecycle are a three piece band from London and this is their debut album Lino Cosmos. These three people produce a unique blend of electronica, breakbeats and psychedelica which creates a sound that is constantly moving, upbeat and simply superb, so I am very excited to bring this to your face today. If you’re into music that gets your hips shaking and your head banging, then this is the album for you baby.
Opening track Dissolve immediately introduces us to this unique sound with a rhythm that sounds like an ocean washing over the over instruments and floods the eardrums with a joyous sound and tells the body to get dancing. This track is upbeat, perfectly structured and quite possibly the best hello I’ve ever heard. It’s the hello that lets you know that you’re about to bond super hard, and that you’re about to become best friends. I am best friends with Lifecycle.
Patterns showcases Lifecycle’s ability to deliver rhythms amongst rhythms as they throw all kinds of percussive elements into this track while also adding the occaisonal extra beat to help bolster it with texture and a little more groove to it. The psychedelic traits shrine through too as the band allow the guitars to let loose just a tad before easing things out into a much more relaxed, beautiful road. The vocals as already heard are top notch and the harmonies just add to this idyllic atmosphere that Lifecycle have managed to produce in a mere three songs.
Lino Cosmos begins to progress towards the psychedelic side of Lifecycle as tracks like Burst Your Bubble and The Big Pictureshow. The Big Picture is a track that features one of the more recognisable guitar riffs on the record, and when it’s paired against the heavier drum beats and the more passionate vocals it really sounds like it’s about to kick your face off. Lifecycle does a great job at shifting their focus onto the other traits of their sound while keeping it relative with the tracks we heard previously.
Our personal highlight has to be Rush Into This. This track is the condensed version of everything that can be heard throughout Lino Cosmos, as it takes the listener on a journey through clouds of loveliness, over the hills of groove while being beaten down by thunderous blows of bass every now and then. This track is heavy, pretty, relaxed and overall, just very badass.
If you’re looking for something different then Lino Cosmos is the album for you. Lifecycle have done a great job at bringing their originality to the masses and have used it to create songs that are very enjoyable and bring a lot of directions and hooks and reasons why you should continue to listen to it every single day. I love this album, it’s amazing. For more music reviews, free downloads and a touch of fangirl check out Exploding Head Syndrome on Facebook and Twitter. | <urn:uuid:e82d3b1e-3f7e-4c31-a514-f09e3f5853ff> | CC-MAIN-2017-47 | https://explodingheadsyndrome.co.uk/2014/11/04/lifecycle-lino-cosmos-album-review/ | 2017-11-21T04:21:40Z | s3://commoncrawl/crawl-data/CC-MAIN-2017-47/segments/1510934806316.80/warc/CC-MAIN-20171121040104-20171121060104-00478.warc.gz | en | 0.950099 | 606 | [
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To have no other goals than physical excellencies, however good and necessary these may be, keeps a man less than he could become. Even to set intellectual and artistic goals is still not enough, however admirable they may be. All these can find their place if they are crowned by the highest excellence of all, which is the spiritual.
-- Notebooks Category 20: What Is Philosophy? > Chapter 1: Toward Defining Philosophy > # 213 | <urn:uuid:dab6b177-e031-4162-9a27-a86a88ce900f> | CC-MAIN-2017-47 | http://paulbrunton.org/notebooks/para/24440 | 2017-11-21T23:10:55Z | s3://commoncrawl/crawl-data/CC-MAIN-2017-47/segments/1510934806438.11/warc/CC-MAIN-20171121223707-20171122003707-00478.warc.gz | en | 0.963523 | 91 | [
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Born near St Louis, Missouri in the sixties, James W. Johnson has spent much of his life exploring the beauty of God`s artistry and creations…..
Photography has always been a strong influence in Johnson`s life from the time he joined his hometown volunteer fire department. Spraying water on the fire, though, was never as important to him as driving and operating the fire trucks and equipment, and photographing fire and rescue scenes. Engineering fire trucks, photography, and helping others in need continue to be strong passions in his life even today.
While attending college, he studied fire science and criminal justice, and actively worked with the Photography Club, studying Fine Arts and learning to develop and print all types of films.
When employment in his field of study looked dismal, Johnson turned to his love of photography and opened his own photofinishing lab and studio operation in the mid 80`s. After twenty years of general portraiture and commercial photographic work, Johnson again changed fields and began another career in emergency services, again helping people in need.
In 1995, Johnson photographed and co-produced a calendar of male and female models intertwined into a firefighting theme, traveled statewide with his models and photographed them at several fire stations and museums to produce the calendar cover as well as the interior layouts. Four posters were also photographed and produced during this time, and were successfully marketed and distributed worldwide.
Some of his commercial work has included photographing male and female models on four wheelers and jet ski watercraft for local dealers. Some of his fire and rescue images also made it inside the pages of Firehouse Magazine over the years of service to his fire department, spanning a total of nearly forty years of service.
Johnson enjoys the beauty found in God`s creations such as sunsets and sunrises, wildlife, landscapes in all seasons, steam trains, waterfalls and fireworks to name but a few. He also restarted his love of collecting minerals and gemstones in the past few years and travels frequently to join other rockhound friends in the pursuit of beautiful treasures. Here you will find many of his adventures chronicled with photos of his newly found treasures. Onyx, his beautiful Border Collie, often travels with him on his adventures. | <urn:uuid:547675a8-5d5b-49e5-9c82-ec64ebe3b91a> | CC-MAIN-2017-47 | http://www.jwjphotoblog.com/?page_id=2 | 2017-11-21T23:04:23Z | s3://commoncrawl/crawl-data/CC-MAIN-2017-47/segments/1510934806438.11/warc/CC-MAIN-20171121223707-20171122003707-00478.warc.gz | en | 0.986287 | 456 | [
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Facts You Must Know About Janitorial Services No one could ever thought of hiring janitorial services to those of the different fast food chains, since they are known to be able to hire their own cleaners to provide needed services, but just recently, a franchise type of fast food establishment renders the need to hire […]
Using the Internet for Reading
When you get to spare some time to read, you will find it much easier for you to always make sure that you are able to gain some knowledge or even able to make sure that you can be able to deal with some of the day to day challenges, meaning, you can be able to know how to solve some problems or even how to become better. There are many means through which you can read, some of them being using the novels or books, they get to make sure that you can also improve on your knowledge, in terms of your grammar or even being able to know something better.
Always get to make sure that you do know of what it is that can get to work for you, so doing makes sure that you can find the best means in which you can be able to gain some knowledge. Getting to know what it is that you would prefer will save your time, meaning that you do not have to always try out various strategies, in life, getting to gain some knowledge and also being able to save on time is something that you need to always stay focused on.
You will therefore get to find that the easiest means in which you will be able to make sure that you do know of how it is that you can learn more is getting to make use of the internet. Furthermore, you get to find that since it has access to all the information, it is much simpler for you to be able to know of a certain book that would be best for you to be able to read, that is, a book in which you can be able to make sure you do know more about something you might have learn in school.
You therefore will have to make sure that you can have the ability of finding the best websites, this is actually simple when using the internet, you get to find that all you will need to do is making sure that indeed you do know of the specific information that you would like and making use of the search engines will get to work to your advantage. Finally, you get to find that you can make use of available articles or even get to make use of the available eBooks which will have all the information in which you can get to desire to solve what it is that you would like.
Partner post: click for more | <urn:uuid:fb5442f8-688f-4ecb-a795-d921e59cb9a6> | CC-MAIN-2017-47 | http://www.stylefashionaccessories.com/what-you-should-know-about-websites-this-year.html | 2017-11-21T23:17:59Z | s3://commoncrawl/crawl-data/CC-MAIN-2017-47/segments/1510934806438.11/warc/CC-MAIN-20171121223707-20171122003707-00478.warc.gz | en | 0.984801 | 529 | [
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Five Detroit legends – most who are living – are going to be honored with a series of “Spirit Murals” at the new Little Caesars Arena.
The murals were painted by Detroit-based artist Don Kilpatrick III. He’s been in a variety of publications an is the chair of the Illustration Department at the College for Creative Studies in Detroit.
According to the Detroit Red Wings, the legends may be rotated or added to depending on what’s going on.
Individuals that have defined sports and entertainment in our community will be changed as important anniversaries come up, allowing us to celebrate great moments and mark the historic milestones that made Detroit swell with pride.
The first five legends are:
- Isiah Thomas – Detroit Pistons’ career leader in points, assists, steals, turnovers, minutes played; led the Pistons to their first Eastern Conference Finals 30 years ago, the deepest they had gone in the playoffs since they moved to Detroit from Ft. Wayne.
- Eminem – Greatest selling male Detroit artist of all time and top selling male artist of the 2000s; the landmark Slim Shady EP recorded 20 years ago.
- Aretha Franklin – The Queen of Soul, one of the most influential and important voices in music history, who was also the first female artist inducted rock-n-roll hall of fame; 50 years ago R-E-S-P-E-C-T became a hit and signature song.
- Gordie Howe – Hockey icon (Mr. Hockey), Detroit Red Wings career leader in games, goals, points; Gordie started wearing his legendary #9 in his second season, 70 years ago.
- Joe Louis – One of the greatest boxers of all time, a nationwide World War II hero who broke the color barrier in professional golf and won the heavyweight championship 80 years ago. | <urn:uuid:8ed307c0-1c44-4f55-a072-f50ca00b1a86> | CC-MAIN-2017-47 | http://www.dailydetroit.com/2017/09/01/5-detroit-legends-honored-spirit-murals-new-arena/ | 2017-11-22T18:33:38Z | s3://commoncrawl/crawl-data/CC-MAIN-2017-47/segments/1510934806620.63/warc/CC-MAIN-20171122175744-20171122195744-00478.warc.gz | en | 0.956243 | 383 | [
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Opinion: An excuse for slashing entitlements - POLITICO.com Print View. With all the talk of Standard & Poor’s downgrade, no one mentioned that the ratings agency’s business model is, essentially, lying for money.
Instead, many politicians insist that the S&P downgrade is the reason for the market turmoil — not the banking freeze-ups in the Eurozone or political paralysis here. The credibility accorded to S&P by U.S. political elites shows that the rot is indeed deep — and worrisome. Continue Reading Let’s note, at the start, that this downgrade was absurd. The credit rating of the United States is not in jeopardy. What’s really happening is an attempt by both parties to justify slashing Social Security and Medicare. President Barack Obama, in his speech Monday about the downgrade, used the market turmoil as an excuse to do just that. In effect, there seems to have been a merger of both parties into a single force advocating for the interests of bondholders and the cutting of Medicare and Social Security. Why trust S&P, anyway? George Packer: The Debt-Ceiling Fight Continues. In the midst of the debt crisis in Washington, D.C., Danny Hartzell backed a Budget rental truck up to a no-frills apartment building that is on a strip of motels and pawnshops in Tampa, Florida.
He had been laid off by a packaging plant during the financial crisis of 2008, had run through his unemployment benefits, and had then taken a part-time job stocking shelves at Target in the middle of the night, for $8.50 an hour. His daughter had developed bone cancer, and he was desperate to make money, but his hours soon dwindled to four or five a week. In April, Hartzell was terminated. His last biweekly paycheck was for a hundred and forty dollars, after taxes.
“It’s kind of like I’ve fallen into that non-climbable-out-of rut,” he said. Reading Between the Lines. The establishment verdict is in: President-elect Bush made an astute choice by tapping Rod Paige, Houston's School Superintendent, to head his Education Department.
The New York Times blessed the nomination as "wise," and both major teachers' unions have chimed in with support. On most of the hot-button questions, Paige is a relatively uncontroversial pick. About vouchers, he has written, "We believe that public funds should go to students, not institutions, and there may be a time when vouchers will be part of the mix.
" (A limited voucher program in Houston was so modest and so narrowly designed that virtually no one took advantage of it.) Paige is a supporter of "performance pay" for teachers and a fairly strong proponent of a skills-based curriculum, especially phonics, but not to the point where he has openly horrified anyone in the teachers' unions or on the educational left. Harold McGraw III. McGraw at the World Economic Forum Annual Meeting, 2011.
Harold Whittlesey "Terry" McGraw III (born 1948) is the chairman of the board of McGraw Hill Financial, formerly McGraw-Hill Companies. He served as chief executive officer of the company from 1998 until November 2013, and was president from 1993 to 2013. The debt deal: Disaster averted, decline straight ahead. This is the best the White House could salvage after inexplicably failing to insist that the debt ceiling be raised as part of December’s deal to extend the Bush tax cuts — which would have let the country avoid this unprecedented exercise in self-inflicted damage?
If you put aside the talking points both sides will peddle, the disappointing contours of the emerging endgame run as follows: First, Washington will do nothing more to boost jobs and growth. The Nauseating Debt-Ceiling 'Solution' - James Fallows - Politics. I agree with Matt Miller: (needless) disaster averted, decline embraced.
>>So this is what we've driven the global economy and America's credit rating to the brink for? Costs of War. How the Deficit Got This Big. The Chart That Should Accompany All Discussions of the Debt Ceiling - James Fallows - Politics. It's this one, from yesterday's New York Times.
Click for a more detailed view, though it's pretty clear as is. It's based on data from the Congressional Budget Office and the Center on Budget and Policy Priorities. Its significance is not partisan (who's "to blame" for the deficit) but intellectual. It demonstrates the utter incoherence of being very concerned about a structural federal deficit but ruling out of consideration the policy that was largest single contributor to that deficit, namely the Bush-era tax cuts. An additional significance of the chart: it identifies policy changes, the things over which Congress and Administration have some control, as opposed to largely external shocks -- like the repercussions of the 9/11 attacks or the deep worldwide recession following the 2008 financial crisis. The point is that governments can respond to but not control external shocks.
But to me it doesn't matter. From this item three months ago: | <urn:uuid:f13348c1-6af5-4769-8f5a-8ae017fcdbb2> | CC-MAIN-2017-47 | http://www.pearltrees.com/francoispearl/debt/id3167612 | 2017-11-22T18:40:49Z | s3://commoncrawl/crawl-data/CC-MAIN-2017-47/segments/1510934806620.63/warc/CC-MAIN-20171122175744-20171122195744-00478.warc.gz | en | 0.961157 | 1,075 | [
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U.S. military aircraft and boats are searching for three U.S. Marines who are missing after their tilt-rotor aircraft crashed into the sea Saturday off Australia's east coast, according to a Marine Corps statement.
Twenty-three other people aboard the MV-22 Osprey have been rescued.
The aircraft had been launched from the USS Bonhomme Richard assault ship and was involved in routine operations when it crashed, the statement said.
A White House official said President Donald Trump, who is vacationing at his golf club in Bedminister, New Jersey, was briefed on the incident by his chief of staff, retired Marine Corps General John Kelly.
The aircraft was still in Australia after completing a joint U.S.-Australian military training exercise two weeks ago in Shoalwater Bay.
The biennial exercise involved about 30,000 troops and 200 aircraft. Australian troops are among U.S.-led coalition forces fighting in Afghanistan and Iraq.
The Marine Corps did not say what caused the crash but indicated it is under investigation.
The Osprey is designed to take off like a helicopter and rotate its propellers to fly like a plane. Its development was almost canceled after 23 Marines were killed during a test flight in 2000.
The U.S. military grounded its Osprey fleet in Japan last December after one of them crash-landed into the sea, injuring five crew members.
Source: Voice of America | <urn:uuid:2be7e17d-0170-47d5-8eef-e5f7d96cca17> | CC-MAIN-2017-47 | https://www.iraqnewsgazette.com/3-marines-missing-after-osprey-crash-off-australian-coast/ | 2017-11-22T18:15:13Z | s3://commoncrawl/crawl-data/CC-MAIN-2017-47/segments/1510934806620.63/warc/CC-MAIN-20171122175744-20171122195744-00478.warc.gz | en | 0.980036 | 295 | [
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Books About Building A Tiny House
As a tiny house enthusiast, we have to tell you about some really well written and informative tiny house books. They are about the designing and building process as well as the small house movement.
List of books: Click the links in the menu to your right.
It’s good information.
We’ve taken a look at a lot of websites and information that is offered about this subject. These are the ones that we’ve found to be the best of the best.
They include information from the history of small houses, to how-to design and build your own home, to debt free living. From general information, to technical information, to helpful resources.
Living in a much smaller house is one of the ways that we have found to reduce our debt load (our debt is melting away very quickly now that we have extra money to pay them off), and simplify our lifestyle. There really is no way to describe how much more comfortable life is without debt (and clutter).
We can honestly say that things are looking up for a change. And we are living a much healthier and happy life.
And it all started when we found and read about this better lifesthyle – one of the tiny house books. | <urn:uuid:d1a1c6f0-c915-451a-9305-37172fa53b05> | CC-MAIN-2017-47 | http://affordablehousingdesigns.com/books/ | 2017-11-23T12:56:18Z | s3://commoncrawl/crawl-data/CC-MAIN-2017-47/segments/1510934806832.87/warc/CC-MAIN-20171123123458-20171123143458-00478.warc.gz | en | 0.965396 | 261 | [
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THE MALL GALLERY
HESKETH HUBBARD ART SOCIETY ANNUAL EXHIBITION
open until June 28
Jiri Keller places his own reflection in the top right hand corner of this unconventional setting. I use the Bakerloo line most days, and I see this work not only as a beautifully executed group painting but also as a shrewd and accurate commentary on changing social patterns. He remarks 'I was motivated to paint when I caught sight of this charming scene in front of me on a tube train. The carriage light giving a good definition of light and shade, and the relaxed behaviour of the family, quite unusual in these surroundings, seemed interesting and worthy of capturing on canvas'.
In this painting his complex task is to convey a moment of warmth and co-operation between three passengers, linked by touch. He captures beautifully the studied concentration of the girl on the right and the mystery of the upward glance of the central figure: is she holding her head high to accommodate what's happening to her hair or is she simply lost in thought and hardly aware of what's going on behind her? And look at the figure on the left. Is that boredom or humour or simple sleepiness?
But there's more. Once upon a time on public transport you did not put your feet on the upholstery but sat demurely the right way up. And kept more or less still. In the past few years the tube has been transformed. It's become a place where most things can happen. Wafts of spicy food creep along the carriage; small children sit in rows with aprons on and are fed meals. It is mesmerising to watch how mascara and blusher and lipstick are applied with mathematical precision and how hair is brushed and sprayed and plaited; nail varnish applied (with its lasting pungent chemical smell). And occasionally some young people use the overhead hand rail to perform acrobatic tricks.
Jiri Keller studied art in Brno, Czechoslovakia, from 1956-1960, immigrating to England in 1969, and has lived in London ever since. He is a member of the British Thematic Association , the UK's national association for theme-based philately and postcards. His collection of art on stamps has grown into a reference library.
The Hesketh Hubbard is London's largest life-drawing society. It has been holding weekly drawing classes since 1930, allowing professional and amateur artists to work in untutored sessions in a variety of styles and media. 110 of their works are on temporary display at The Mall Gallery, a champion of figurative art by living artists. | <urn:uuid:76d60ff7-cd17-45cb-a77d-5a910ddd9c42> | CC-MAIN-2017-47 | http://walktofreeartlondon.blogspot.com/2014/06/288-on-bakerloo-line-by-jiri-keller.html | 2017-11-23T12:58:58Z | s3://commoncrawl/crawl-data/CC-MAIN-2017-47/segments/1510934806832.87/warc/CC-MAIN-20171123123458-20171123143458-00478.warc.gz | en | 0.960197 | 540 | [
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Independent Ink was founded in 1996 and is currently owned by Joshua Ortega-Scheiner. We specialize in custom artwork and if you need anything we will work with you. If you have a design in mind or a pattern, bring it in and we will get it done. As of this time we are no longer offering Piercings, we will be doing Tattoos only. If you need to browse we also have a showroom of flash and design. Please feel free to come on by. Our parking is in the back as crazy at it might be. | <urn:uuid:b39b3969-c29c-41f5-a152-281de69d5b4a> | CC-MAIN-2017-47 | http://www.spitfireabq.com/?page_id=10 | 2017-11-24T07:40:39Z | s3://commoncrawl/crawl-data/CC-MAIN-2017-47/segments/1510934807146.16/warc/CC-MAIN-20171124070019-20171124090019-00478.warc.gz | en | 0.980276 | 114 | [
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Funded in part by Winn Feline Foundation grants (W10-014, W11-041), the George and Phyllis Miller Trust through Winn (MT08-015, MT13-010), and Winn participation in the Cat Health Network (D12FE-510).
An international team of geneticists and neurologists from University of Missouri, University of California-Davis, University of California-San Diego, University of Sydney and the University of Milan has recently discovered the recessive mutation causing a congenital muscle weakness (myopathy) in Devon Rex and Sphynx cats. The gene, COLQ, affected by the mutation leads to a congenital myasthenic syndrome (CMS) similar to humans. A recessive mutation requires two copies of the mutation to have disease.
Devon Rex was the breed most likely affected initially and then spread to Sphynx. Signs in affected cats are passive ventroflexion of the head and neck, head bobbing, scapulae protrusion, megaesophagus, generalized muscle weakness and fatigability. Prominent spinal curvature (lordosis) can occur and the affected cats usually die by two years of age due to secondary complications by asphyxiation due to choking on food or aspiration pneumonia. Onset of signs ranges from 3 to 23 weeks of age and then progresses slowly or remains unchanging.
Additionally, cats with moderate to severe disease show signs of generalized muscle weakness, especially following periods of exertion, stress or excitement. There will be other typical signs of a high-stepping forelimb gait, head bobbing and progressive dorsal protrusion of the scapulae (shoulders). As affected cats tire more with exercise, they will have a shortening of stride and superimposed tremor until they eventually collapse in sternal recumbency. Their head will rest on or alongside their front paws. They may also assume a characteristic position for this condition – “dog-begging or “chipmunk” style- with their front legs resting on a nearby convenient object.
The disease appears to be recognized world-wide. The condition was initially noted and reported in 1989 in the United Kingdom. A few years later in1993, presentations were detailed in Australia. Cats with the disease have been found in the USA and more recently across Europe. (VT)
Shelton GD, Sturges B, Lyons L, Williams DC, Aleman M, Jiang Y, Mizisin A (2007) Myopathy with tubulin-reactive inclusions in two cats. Acta Neuropathol 114 (5):537-542.
Martin PT, Shelton GD, Dickinson PJ, Sturges BK, Xu R, LeCouteur RA, Guo LT, Grahn RA, Lo HP, North KN (2008) Muscular dystrophy associated with α-dystroglycan deficiency in Sphynx and Devon Rex cats. Neuromuscular Disorders 18 (12):942-952 | <urn:uuid:186e5612-0ae8-4afe-8d3b-7547aaa7f90e> | CC-MAIN-2017-47 | http://www.winnfelinefoundation.org/education/cat-health-news-blog/details/cat-health-news-from-the-winn-feline-foundation/2015/06/23/an-answer-on-spasticity-in-devon-rex-and-sphynx | 2017-11-24T07:24:21Z | s3://commoncrawl/crawl-data/CC-MAIN-2017-47/segments/1510934807146.16/warc/CC-MAIN-20171124070019-20171124090019-00478.warc.gz | en | 0.917909 | 617 | [
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It's easy to get excited about the promise of the Internet of Things (IoT). But translating that excitement into real live IoT products and solutions that delight customers is anything but easy.
In fact, it's really difficult because the problem is inherently complex - an IoT solution spans hardware, software, cloud, security, applications, and big data analytics.
And yet, customers expect connected products that blend into their lives or business workflows.
In this eBook, we explore what it truly takes to build an IoT solution, and the questions device manufacturers must answer to determine the right approach for your organization. | <urn:uuid:63fb55a9-5b53-400e-a81e-0d3b275704f4> | CC-MAIN-2017-47 | https://www.artik.io/buildvsbuy?utm_campaign=buildvsbuy&utm_source=staceyknowthings | 2017-11-24T07:27:56Z | s3://commoncrawl/crawl-data/CC-MAIN-2017-47/segments/1510934807146.16/warc/CC-MAIN-20171124070019-20171124090019-00478.warc.gz | en | 0.93982 | 123 | [
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Well what an absolutely gorgeously sunny day today. That's what summer should be like. It's my youngest's birthday today. Jordan is going to be fourteen close to midnight. He's fast changing from my 'baby' into a young man. Mind you, his favourite pastime, besides his BMX bike, is playing Band Hero on the Wii, so he's not quite into the man brigade yet.....or is he. lol xx
Anyway, I wanted to capture something that relates to him, and I think this card is perfect.
I wanted a really grungy, rock look, and I think I've achieved it. All the cardstock is from Stampin' Up!...I distressed the edges of the cardstock. and used lots of CloseTo Cocoa ink to give that grungy look. I embossed the guitar with moonglow embossing powder so it looks all glossy just like jordan's real guitar. Used embossing folders for the punched out stars, just to give interest, along with some rivets from Stampin' Up!
There's quite alot going on in this card, and I think it maybe looks better in 'real life'. But I hope you get a good impression of it anyway. It's hard doing teenage boys cards, and not making them too cute or babyish. Jordan liked the card anyway, so that's all that matters. Thanks you for taking the time to look.
My next task is to make a card for the flowers I've chosen for my brother-in-laws funeral this Friday. He was only 59 years old. Rest In Peace Brian, you will be sadly missed. | <urn:uuid:07c6296b-10b4-481e-85dd-1f875ab8eefb> | CC-MAIN-2017-47 | http://pinkpoppycraftbox.blogspot.com/2010/06/birthday-boy.html | 2017-11-25T02:09:43Z | s3://commoncrawl/crawl-data/CC-MAIN-2017-47/segments/1510934809229.69/warc/CC-MAIN-20171125013040-20171125033040-00478.warc.gz | en | 0.977444 | 339 | [
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Following their roster change yesterday, the officials at ELEAGUE have cleared the air and disqualified OpTic from this season. The move came in soon after OpTic signed allu, friberg and Magisk in their lineup.
The official statement of Turner Sports, the organization which runs ELEAGUE, clearly labeled this as “inconsistent with ELEAGUE rules” which says, “if more than two players on a team’s roster are changed, the team will be removed from the tournament immediately.”
The determination of the team taking the sport of OpTic will be finalized on the American Open Qualifiers of ELEAGUE, scheduled on 26th and 27th August.
Ironic it may sound, OpTiC was the champions of ELEAGUE Season 2 where they beat Astralis 2-1. | <urn:uuid:16574726-d649-4811-9d10-b021f9d2259c> | CC-MAIN-2017-47 | http://www.talkesport.com/news/csgo/optic-disqualified-eleague/ | 2017-11-18T02:58:26Z | s3://commoncrawl/crawl-data/CC-MAIN-2017-47/segments/1510934804518.38/warc/CC-MAIN-20171118021803-20171118041803-00678.warc.gz | en | 0.966451 | 175 | [
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Today’s Photo: The Rocky Point
I was walking the trail to the base of Fall Creek Falls when I ran across this prominence. I was drawn to it because there were a couple teenagers sitting right at the edge where the tree sticks out. It reminded me of all the time I spent rock climbing when I was younger. If I had taken any of my climbing gear, I would have been hard pressed to stay away from this. As it was, I had to settle with a few photos. | <urn:uuid:d2c48ecd-1614-4866-b518-b6946a399f84> | CC-MAIN-2017-47 | http://justshootingmemories.com/2013/01/12/the-rocky-point/ | 2017-11-18T21:21:38Z | s3://commoncrawl/crawl-data/CC-MAIN-2017-47/segments/1510934805049.34/warc/CC-MAIN-20171118210145-20171118230145-00678.warc.gz | en | 0.992168 | 102 | [
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Derby arts centre QUAD will be hosting FreeFall Arts’ Past Lives Derby film, presented with live musical score from internationally renowned musicians, working with members of Sinfonia Viva plus local young musicians.
Past Lives Derby film, screening with live musical accompaniment takes place on Saturday, June 13, from 2pm to 3.30pm. Tickets are £8 or £5.50 concessions.
The screening will be followed by a Past Lives Reminiscence Vintage Café where audience members from the screening are invited to share their thoughts and memories of Derby and responses from the Past Lives film over tea, coffee and cake. There will also be an exhibition of archive Derby photos in the foyer. Past Lives Reminiscence Vintage Café takes place on June 13, from 3.30pm to 5.30pm. The event is free but places are limited, so please book tickets.
Past Lives Project partners with archives across the Midlands to ensure that local movies, photos and oral history digitised through the project will stay publicly accessible and preserved for future generations, and to encourage people interested in heritage to connect to their local, regional and national archives. | <urn:uuid:6fe141ad-1167-4f72-be0e-07ac86d405b8> | CC-MAIN-2017-47 | http://www.belpernews.co.uk/whats-on/arts/archive-film-treat-at-quad-1-7292792 | 2017-11-18T21:26:14Z | s3://commoncrawl/crawl-data/CC-MAIN-2017-47/segments/1510934805049.34/warc/CC-MAIN-20171118210145-20171118230145-00678.warc.gz | en | 0.931105 | 236 | [
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Ever since we got back from Greece, I crave Mediterranean food. Something about delicious tzatziki sauce and gyros takes me right back to Mykonos and Santorini. Fortunately, I found a place in Plano that offers fresh and flavorful food that satisfies that craving. Taziki’s Mediterranean Cafe has two locations in DFW, Plano and Southlake. The Plano location is just south of 121 and Preston. Plano Magazine and Tazikis Plano partnered together to host a fit foodie night with several Dallas and Plano bloggers.
Taziki’s started in Birmingham, Alabama and now has over 40 locations in 11 states. They pride themselves on being the first national franchise to receive a REAL certification. You will not find a microwave, freezer or deep fryer in their kitchen, because all of their food is made with the freshest ingredients. They are a scratch kitchen that offers healthier prep methods too. Not only can you choose from one of many freshly grilled meats, but there are a variety of vegetarian options. Taziki’s is a great place for all plant-based and meat eaters alike.
What to eat
No matter what you order from the menu, know that it will be good, fresh, and flavorful.
Choose from a variety of dips- hummus, taziki, or spicy pimento cheese (my favorite!).
Entrees + sides
The grilled chicken roll up has feta and tomato and served in a griddled tortilla.
Any of the grilled meats make an excellent entree choice, too. Choose from chicken, beef, lamb, or pork loin. All meats are served with Greek salad and your choice of rice or potatoes.
They even have seafood options like salmon, shrimp, or tilapia.
If you are looking for less meaty options, choose from a variety of salads or the grilled veggie plate.
Accompany your entree with one of their fresh sides. I highly recommend the pasta salad or tomato-cucumber salad.
The dark chocolate cake is perfect for the chocolate lover of your group. It’s made from scratch with Richmond icing.
As much as I love chocolate, the baklava is where it’s at. They get their baklava from Hellas bakery, and it is by far the best tasting baklava I have ever had.
Head over to Taziki’s for lunch or dinner the next time you want some REAL delicious food.
What do you want to try? What’s your favorite Mediterranean dish? Leave me a comment below. | <urn:uuid:6c92e2fe-d2a9-4fce-9376-222cefc149b2> | CC-MAIN-2017-47 | http://www.loveyoumoretoo.com/tazikis-mediterranean-cafe/ | 2017-11-18T21:21:16Z | s3://commoncrawl/crawl-data/CC-MAIN-2017-47/segments/1510934805049.34/warc/CC-MAIN-20171118210145-20171118230145-00678.warc.gz | en | 0.935252 | 549 | [
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Thanks to ThreeIfBySpace.com for this article!
Season 3 is still 8 months away, but fans have been coming up with some very interesting theories on what might be coming for the 2nd mass and the new alien race revealed at the end of season 2. The 2nd mass made it to Charleston but it wasnt the haven they had hoped for and they had some heavy losses in a fight to take out an overlord weapon that Red Eye and Ben assured them must be taken out. Dai was lost and so was red-eye which left many fans upset as Dai was a fan favorite of the series since season 1.
Tom questioned the purpose of this weapon and why it was pointed to the sky and not to earth. Ben hints at the fact that it wasn’t mean for humans. The final moments of season 2 gave us an answer as to what it was there for. This weapon was pointed up to prevent these new race of aliens from landing. Now that they have landed, the question remains are they an alley or an enemy? This question has multiple possible answers.
In Molon Lobe, the overlord reveals a cliché line that earth is out of control and they were there to ‘correct’ their course. However many fans, including us felt this was all misinformation. It appeared as though the overlords or Ashpheni were running away from something and using earth as a staging ground. They can’t fight back against another race of advanced beings when their slave race is able to disobey orders. That’s where the harnesses come in.
We have seen a kid harnessed and we have seen with Ben what these harnesses start to do to the kids. It appears the overlords use the harness not only to control the mind and actions of the host but also to change them, creating an exoskeleton that would make them the perfect solider. Why not fight their own battles? The Ashpheni do not use storage devices and run huge sections of their global operations via their mind and communication with each other. That means they must keep what they say to the skitters or the kids at a minimum or risk plans being leaked to the new rebellion. That also means the Ashpheni would not show themselves on the battlefield or risk capture or death. | <urn:uuid:2d7ae53f-eb60-493f-bd5e-abddae729e68> | CC-MAIN-2017-47 | https://nyrhalahotep.wordpress.com/2012/10/25/falling-skies-season-3-whats-ahead-for-the-2nd-mass-three-if-by-space/ | 2017-11-18T21:10:46Z | s3://commoncrawl/crawl-data/CC-MAIN-2017-47/segments/1510934805049.34/warc/CC-MAIN-20171118210145-20171118230145-00678.warc.gz | en | 0.986149 | 470 | [
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My favorite Christmas stories are about service to others, so I thought today, I’d share about ways that Austen’s characters help the poor and needy. However, as I brainstormed about the various stories, one character stood out as a stellar example–so much so that I couldn’t help comparing him to Dickens’ Mr. Fezziwig. That character is Sir John Middleton.
In the beginning of Sense and Sensibility, Austen illustrates how Fanny Dashwood’s selfish ways can make a miserable family even more miserable. Fanny is rather a Scrooge, don’t you think? She doesn’t want the Dashwoods to have any of the money her husband inherits. But, luckily, as Mrs. Dashwood has reached a point of despair, a letter from Sir John arrives:
“It was the offer of a small house, on very easy terms, belonging to a relation of her own, a gentleman of consequence and property in Devonshire. The letter was from this gentleman himself, and written in the true spirit of friendly accommodation. He understood that she was in need of a dwelling; and though the house he now offered her was merely a cottage, he assured her that everything should be done to it which she might think necessary, if the situation pleased her. He earnestly pressed her, after giving the particulars of the house and garden, to come with her daughters to Barton Park, the place of his own residence, from whence she might judge, herself, whether Barton Cottage, for the houses were in the same parish, could, by any alteration, be made comfortable to her. He seemed really anxious to accommodate them and the whole of his letter was written in so friendly a style as could not fail of giving pleasure to his cousin.”
Sir John is equally friendly in person:
“His countenance was thoroughly good-humoured; and his manners were as friendly as the style of his letter. Their arrival seemed to afford him real satisfaction, and their comfort to be an object of real solicitude to him. He said much of his earnest desire of their living in the most sociable terms with his family, and pressed them so cordially to dine at Barton Park every day till they were better settled at home, that, though his entreaties were carried to a point of perseverance beyond civility, they could not give offence.”
I love that Sir John isn’t a great example of etiquette. The fact that he’s not perfect is comforting, a reminder that even average people like me can bring joy to others.
As the story continues, it’s obvious that Sir John brings much happiness to the Dashwood family. He introduces them to other people, invites them to dinner, takes them on excursions, visits them frequently, and holds balls. Yes, it is painful for Elinor to meet Lucy Steele, and Sir John’s teasing is annoying, but what would the Dashwoods have done without him, that “benevolent, philanthropic man”? | <urn:uuid:bad1a0f1-3c1a-44a4-8526-69a7f246e93c> | CC-MAIN-2017-47 | https://austenauthors.net/sir-john-middleton-a-right-jolly-old-elf/ | 2017-11-19T15:46:58Z | s3://commoncrawl/crawl-data/CC-MAIN-2017-47/segments/1510934805687.20/warc/CC-MAIN-20171119153219-20171119173219-00678.warc.gz | en | 0.98674 | 642 | [
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…..So we’re here for you every step of your career. When you feel supported and connected, you can do great things. So we help you – effortlessly – stay up to date and learn from the best.
Welcome to your place to learn, grow and prosper. The latest news is curated right here.
Along with job listings from far and wide, delve deep into our products and resources, or join an event near you. Learn on the go with our podcasts, or get a front-row seat at one of our webinars. Our community is over 6,000 strong, and we’re looking forward to meeting you.
“I founded Dietitian Connection because I saw a need.
There were resources for those who sought to become a better leader – and resources for those who wanted to become better at the practice of dietetics.
Nowhere was there a single, one-stop shop for dietitians who sought to be inspired, share ideas, educate and engage with like-minded professionals.
I’ve endeavoured to fill that gap for our industry by founding Dietitian Connection.
Today, we’re a dynamic community of innovative, motivated nutrition leaders, enjoying exceptional opportunities for professional growth and advancement.
Every new member enriches our community in their own unique way.
Won’t you be next?”
Maree Ferguson, Founder Dietitian Connection | <urn:uuid:2eaf105a-4d7f-483b-b599-db0178bf8b4d> | CC-MAIN-2017-47 | https://dietitianconnection.com/about/welcome/ | 2017-11-19T16:19:47Z | s3://commoncrawl/crawl-data/CC-MAIN-2017-47/segments/1510934805687.20/warc/CC-MAIN-20171119153219-20171119173219-00678.warc.gz | en | 0.948999 | 297 | [
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. . .when I strongly suggest that you to check out the The David Lee Roth Running With The Devil Soundboard. It’ll make your pants tighter.
The rock vigilance award for this discovery goes to my L.A. buddy Bloomer. Bloomer is one of the country’s great men. In addition to somehow insinuating himself into the highest levels of the defense contracting industry, his life accomplishments include the creation of the regionally famous “porn guitar.” You never did see such a thing as the porn guitar. It remains a defining symbol of early 1990s Western New York drunken malaise. | <urn:uuid:09d99bf8-b81b-48ef-8186-77e805a3ca5c> | CC-MAIN-2017-47 | http://www.thecontrarianmedia.com/2009/01/goddamnit-baby-you-know-i-aint-lyin-to-ya/ | 2017-11-20T11:58:41Z | s3://commoncrawl/crawl-data/CC-MAIN-2017-47/segments/1510934806030.27/warc/CC-MAIN-20171120111550-20171120131550-00678.warc.gz | en | 0.921137 | 130 | [
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I think that I shall never see
A poem lovely as a tree.
Joyce Kilmer wrote that. Or maybe it was Samuel Oscar Blanc, who founded Roto-Rooter.
Regardless, today is National Arbor Day. Let us celebrate trees and remind ourselves that sometimes history grows thereon:
For example, if, ala the metal detector and the Geiger counter, there were such a gizmo as a history detector, a sweep around this tree would send the detector’s needle off the dial. This live oak tree on Samuels Avenue bends low to the ground, as if weary. It’s entitled. This is Traders Oak, and it is Fort Worth’s family tree—been here since day one. And even before day one. In fact, Traders Oak probably was already middle aged in 1849 when Henry Clay Daggett (first of the three Daggett brothers to reach Fort Worth) and Archibald Leonard arrived to set up a trading post near the Army’s new fort.
Army regulations prohibited merchants from doing business within one mile of a fort, so the two traders built a log cabin at this big live oak and began Fort Worth’s first business. The location was perfect: soldiers for customers, soldiers for protection.
Indians also came to the store to trade pecans, furs, and buffalo hides. The tree became a center of community in those early days. A nearby spring provided water. After Tarrant County was created from sprawling Navarro County in 1849, the first county election was held at the tree in 1850. County officials were elected, and voters were asked to select the site of a county seat, which, the state decreed, would be named “Birdville” after Major Jonathan Bird, who had established Bird’s Fort on the Trinity River in 1841 in what is now north Arlington. A settlement ten miles west of Bird’s Fort won the election and became Birdville, the county seat. But in 1856 Fort Worth boosters talked the state legislature into calling a second election for county seat and on election day lured voters to the polls by serving free whiskey. Depending on which historical account you read, Fort Worth won the Birdville-Fort Worth rematch by either seven or thirteen or (hic) twenty-six votes.
Shades of early Cowtown: After the Army vacated its Fort Worth in 1853, another Daggett brother, Ephraim, converted the fort’s stable into a lodging house in the nascent town of Fort Worth. But he soon sold the property to Lawrence Steele, who built on the site a two-story hotel, Steele’s Tavern. The hotel also had a stage coach office. Passengers could buy tickets and wait for the stage in the parlor or the sitting room. The first stage, carrying mail, arrived in July 1856. Today this three-trunked live oak, which provided shade first at the fort and then at Steele’s Tavern, shades pedestrians at Belknap and Houston streets. Just a few feet from the tree is a marker commemorating the town’s first school, established by John Peter Smith in 1853 in the “pest hospital” (for communicable diseases) of the abandoned fort.
And in a parking lot three hundred feet south of the Modern Art Museum of Fort Worth stands the John Peter Smith Oak. Why is Smith honored with a tree at that particular location?
Because there is much history under the concrete of that museum parking lot. Smith, in addition to starting Fort Worth’s first school, was instrumental in founding the town’s public school system in 1882. And in 1955 that public school system built its administration building just south of that tree.
And just east of the John Peter Smith oak was a building that some remember as Van Zandt Elementary School. (Photo from Billy W. Sills Center for Archives/FWISD.)
But the building, designed by Wiley Clarkson, began life in 1922 as West Side High School (also called “Van Zandt High School”). The school was in the Van Zandt school district (Major Khleber Miller Van Zandt had lived nearby and owned a huge amount of land in that area). But the Van Zandt school district used the building as a high school only briefly because later in 1922 the Fort Worth school district annexed the Van Zandt school district and designated West Side High School as “West Van Zandt Elementary School” (not to be confused with Van Zandt School of the Fifth Ward). The elementary school closed in 1964 because of low enrollment, and the building became an auxiliary of the main FWISD administration building. The Van Zandt building was torn down in 1986, the main administration building in the late 1990s.
He-was-no-fool’s gold: Captain Charles Turner was one of Colonel Middleton Tate Johnson’s Texas Rangers. In 1849 Turner accompanied Johnson and Major Ripley Arnold as they scouted for a suitable location to establish Fort Worth. Later Turner owned a farm where Greenwood Cemetery (1909) now is. When the Civil War began, the Confederacy required its citizens to exchange their gold for Confederate notes. Turner had a better idea: With the help of a trusted slave, Turner buried his gold—believed to have been thousands of dollars’ worth—under a live oak tree on his farm. After the war Turner dug up the gold and used it to help Fort Worth’s struggling economy and to help the city pay debts to creditors in the North. Today the Turner Oak stands in a traffic circle in the cemetery.
The traders took a powder: Ed Terrell of Kentucky was one of the first white settlers in what would become Tarrant County in 1843. “There were more panthers in these parts than I have ever seen before or since; antelopes without number, wild turkeys in every tree—in fact, in those days this was God’s own country,” Terrell recalled years later. It was also Indian country. In 1844 Terrell and trapper John Lusk, from Arkansas, traded with local Indians—beads and trinkets for buffalo robes and other pelts. Planning to build a trading post on the Trinity, the two men selected a site in a live oak grove where Sports Rehab Specialists now stands at Cooper Street and 11th Avenue. They cut down trees and trimmed the logs. But they needed help lifting the logs into place. So, they headed east to fetch two other traders about thirty miles away in Dallas County. But along the way Indians captured Terrell and Lusk. After about a year in captivity, the two men tricked the Indians: Terrell, who baked biscuits for the Indians, told them that he was out of flour and needed to go get more. As the story goes, the Indians freed the two men after the two men promised to return.
Terrell hightailed it out of Tarrant County and didn’t return until 1849 after the fort was established. He operated the First and Last Chance Saloon in a log cabin near the courthouse and was elected the city’s first marshal in 1873.
Yes, history grows on trees. And sometimes so do bicycles: Years ago Grand Avenue homeowner George Hilton started his bike tree. (Update: The tree is still there, but the bicycles are gone.)
Not to be confused with Steele’s tree are these steel trees. This sculpture by Roxy Paine at the Modern Art Museum of Fort Worth is entitled Conjoined.
And just for good measure, there’s the Memorial Oak, tucked away near the second fairway of Pecan Valley Golf Course in southwest Fort Worth. It is the Texas state champion bur oak.
The Memorial Oak is eighty-one feet high, nineteen feet in circumference (almost six feet in diameter). (You knew that Texas would have an official registry for biggest trees, didn’t you?)
But the Memorial Oak holds the record for bur oaks in Texas no thanks to some vandals who in 2011 took an ax to the tree. A scar can be seen above the bicycle.
Was this pecan tree in the median of Bellaire Drive South a trail marker tree formed by Indians? Some residents of the Overton Woods neighborhood believe so. The website Heritage Trees of Fort Worth, presented by the Forestry Section of Fort Worth Parks and Community Services Department, says: “These nomads would follow large herds of animals and are known to have tied the tops of saplings to the ground in locations near crucial resources and along important routes. As the trees matured, the bend would stay in place and a navigation landmark was created. This pecan points to the nearby Trinity River and is believed to have guided the Native Americans to a reliable water source.” (Thanks to Paul Valentine for the tip.)
Lastly, let us remember five trees that no longer stand or at least can’t be identified, two with a negative story to tell, three with a positive story to tell:
1. The tree from which William H. Crawford and Anthony Bewley were hanged in 1860.
2. The tree from which Tom Vickery was hanged in 1920 and Fred Rouse was hanged in 1921.
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With the end of the tax year just 3 weeks away, there are still steps one can take to reduce your IHT:
- Maximise the £3,000 annual contribution
- Consider Making Gifts
- Check whether your life insurance policies are under trust
- Consider the benefits of using discounted gift trusts and loan trusts
Need advice? We are here to help. | <urn:uuid:51c98a3a-8b1b-4cc4-8806-2d1aead2a52c> | CC-MAIN-2017-47 | http://investment-solutions.co.uk/blog/2016/3/14/end-of | 2017-11-21T06:23:41Z | s3://commoncrawl/crawl-data/CC-MAIN-2017-47/segments/1510934806317.75/warc/CC-MAIN-20171121055145-20171121075145-00678.warc.gz | en | 0.938346 | 75 | [
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OAKLAND PARK, Fla. (AP) — Deputies in Florida say a thief worked for 15 minutes to drag a cooler full of Good Humor ice cream past a sleeping clerk at a gas station near Fort Lauderdale.
Surveillance cameras captured a man walking up to the Oakland Park store at 4:09 a.m. on April 2 to find the clerk sleeping.
Authorities say the man opened the ice cream cooler, peered at the clerk, looked directly into the camera and flashed a middle finger.
After a few minutes, he carefully pulled the $2,500 cooler through the store's doors.
Authorities arrested Dennis Norman on grand theft charges on April 22. He was released from jail on $1,000 bond. There was no phone number available for Norman. | <urn:uuid:9e6986b1-7049-4619-a6b6-3a9ca7735304> | CC-MAIN-2017-47 | http://www.mcall.com/news/nationworld/mc-florida-ice-cream-thief-0424-20150424-story.html | 2017-11-21T07:06:09Z | s3://commoncrawl/crawl-data/CC-MAIN-2017-47/segments/1510934806317.75/warc/CC-MAIN-20171121055145-20171121075145-00678.warc.gz | en | 0.938819 | 160 | [
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If you are interested in working with Home Little Home, we would love to hear from you!
Whether you’re interested in seeing a review of your book or product featured in the magazine or contacting us about features, there are a number of ways we could work together. Here at HLH we love testing out new materials for projects, writing about new and exciting interiors and craft books and have a growing audience keen to see the shiniest stuff on the lifestyle market through book extracts, product reviews and feature articles.
Or if you’d like to share your own latest projects and fancy a chat, we’d love to hear from you too!
Get in touch! | <urn:uuid:16b2fdcf-783e-4d1c-bf1f-ca36c23dc7fc> | CC-MAIN-2017-47 | http://homelittlehome.com/contact/ | 2017-11-22T02:03:12Z | s3://commoncrawl/crawl-data/CC-MAIN-2017-47/segments/1510934806447.28/warc/CC-MAIN-20171122012409-20171122032409-00678.warc.gz | en | 0.943995 | 141 | [
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16/04/2004 - Member countries of the OECD's Development Assistance Committee (DAC) increased their official development assistance (ODA) to developing countries by 3.9% in real terms from 2002 to 2003, following a 7.0% real increase between 2001 and 2002. These "real terms" data are adjusted both for inflation and for the large fluctuations in exchange rates over the past two years.
According to preliminary data total DAC ODA in 2003 reached $68.5 billion, t e highest level ever, both in nominal and real terms. This total represented 0.25% of DAC members' combined gross national income (GNI), up from 0.23% in 2002 and 0.22% in 2001. Three major factors are behind the $2.3 billion rise, in real terms, in 2003:
The United States remains the world's largest aid donor in volume terms, followed by Japan, France, Germany and the United Kingdom. Denmark, Luxembourg, the Netherlands, Norway and Sweden are still the only countries to meet the United Nations ODA target of 0.7% of GNI. Three other countries have given a firm date to reach the 0.7% target: Belgium by 2010; Ireland by 2007; and France to reach 0.5% by 2007 and 0.7% by 2012.
Twelve of the twenty-two DAC member countries reported an increase in ODA in real terms. In 2003, the United States increased its ODA by 16.9% in real terms to $15.8 billion in 2003, representing 0.14% of its GNI. US bilateral aid jumped by $3.9 billion, including $2 billion for Iraq, partly offset by a $1.4 billion fall in US multilateral aid due to the timing of capital subscriptions to international financial institutions.
Japan's ODA fell by 8.9% in real terms, to $8.9 billion, representing 0.20% of its GNI. While gross disbursements remained relatively steady, there was a substantial increase in repayments of ODA loans.
EU countries increased their ODA in 2003 by 2.2% in real terms, representing 0.35% of their combined GNI. Prior to Monterrey, EU Members committed to increase their ODA by 2006 collectively to 0.39% of GNI, and individually to a minimum of 0.33% of GNI. Features of EU aid included:
Other DAC countries showed the following changes in real terms in ODA in 2003:
Among the non-DAC donors, Korea's ODA rose in current dollars from $279 million in 2002 to $334 million in 2003.
For further information, journalists are invited to contact Helen Fisher, OECD Media Relations Division (tel. 1 45 24 80 97). | <urn:uuid:5c96ed31-f6d1-4549-ae02-e98318fbb67f> | CC-MAIN-2017-47 | http://www.oecd.org/general/modestincreaseindevelopmentaidin2003.htm | 2017-11-22T02:05:07Z | s3://commoncrawl/crawl-data/CC-MAIN-2017-47/segments/1510934806447.28/warc/CC-MAIN-20171122012409-20171122032409-00678.warc.gz | en | 0.943102 | 576 | [
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This is really good, When i listen to it i think of an awesome level in a videogame for some reason.. but def keep it up :)
I love it!
This my duuudde is a winner haha i am throwing it on replay :)
I love this :)
I madly agree with "The-Animus" def keep the vocals going, possibly echo/delay them out ...and maybe add a more dominant sound in the beginning like rain, wind...a subway train..etc just something that creates an image in the listeners head :)
But its your song and i def dig it regardless... looking forward to hearing more tunes from you
newgrounds.com — Your #1 online entertainment & artist community! All your base are belong to us. | <urn:uuid:eb58927d-d4ea-48e8-808b-055c185a8953> | CC-MAIN-2017-47 | https://tissueboxhero.newgrounds.com/reviews/ | 2017-11-22T01:34:50Z | s3://commoncrawl/crawl-data/CC-MAIN-2017-47/segments/1510934806447.28/warc/CC-MAIN-20171122012409-20171122032409-00678.warc.gz | en | 0.916855 | 157 | [
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Monday, March 28, 2011
This is 24x24 on wood.. This has been one that I would work on and set aside..I would add a layer and then I knew I should just sit it aside and wait..It did not feel right..Finally the moment came and I knew it was complete so I signed it...Title is" Protection"....We all want to feel protected and safe from different things.
For some it is pain and from some it is just fear of different things, and others it is depression..We all have our reasons..
Then there are other people who want to protect...As a mother wants to protect her children and then there is God who wants to protect us all and keep us safe near him. To keep us from harm and evil..
The light is terrible in the shot and I know you can not see the color I hope you can see it enough.
I hardly ever sale anything..I just want to keep them because of fear and also we put so much of our self into our art. It is not like I do not need the money..lol.. I just suppose I am protecting my self from the fear of being rejected as an artist.. FEAR is a terrible word and Protection is such a wonderful word..
Enjoy your week and I hope for protection to all and may it keep your fears and troubles away.
Saturday, March 19, 2011
The weather here is wonderful...Warm, take your shoes off and walk in the cool grass...Birds flying, flowers blooming and trees waking up and stretching their arms.. Spring Time......
Enjoy the weekend.
Thursday, March 10, 2011
I have had a little time to step into my studio..It almost seems like it belongs to someone else. I sit down in my chair and look around. I pick up this and that and lay it all aside. I get up and I walk around. I start to walk out and then I step back and take one more look around. I see different types of canvas laying around..Some that have paint on them some of them still white with no beginning. I start to touch some of the ones that have beginnings.
I pick up this piece you see now..I had started it along time ago. It is done on canvas board 12x15.
So I picked up the charcoal and started to see something or could I feel it..It does not matter because my hand just started to move..I suppose it is the mood of an artist that shows in our work..I am in the shadows of life but it is where I belong. God is always there even if we are in the shadows..He will bring us out when it is time..His timing not ours.
Thanks for stopping by and I always enjoy the comments and the visits..I hope I can get around to visit. I do miss seeing all the wonderful art and reading all the words of poetry and stories of your life.
Take care and remember...May you always dream in color. | <urn:uuid:bbab086d-bd15-448d-8b78-8327ad86cfbd> | CC-MAIN-2017-47 | http://wordsofapoeticartist.blogspot.com/2011/03/ | 2017-11-22T20:32:21Z | s3://commoncrawl/crawl-data/CC-MAIN-2017-47/segments/1510934806660.82/warc/CC-MAIN-20171122194844-20171122214844-00678.warc.gz | en | 0.975685 | 616 | [
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Wouldn't it be nice if you could eat whatever you wanted, blow off working out and still fit in your skinny jeans? While that's unlikely, it is possible to speed up your metabolism, that little engine in your body that burns calories. As you age, your metabolism slows down, chiefly because you lose around a half pound of muscle each year. "If you don't use the muscle, it atrophies," explains Pamela Peeke, M.D., professor of medicine at the University of Maryland and author of Fight Fat After Forty. That translates into burning about 400 fewer calories each day, which could mean gaining a pound a week. But there's plenty you can do to rev your fat-burning furnace back up again, says Peeke. Here's the skinny:
Stock up on green tea
Green tea isn't known only for its cancer-fighting benefits: It may help boost your metabolism, too. People who took green-tea extract three times a day saw their metabolic rate increase by about 4 percent, according to a study published in the December 1999 American Journal of Clinical Nutrition. (Translation: You could burn an extra 60 calories a day, which equals about six pounds a year!) It may be because green tea contains catechins, which increase levels of the metabolism-speeding brain chemical norepinephrine, says Joy Bauer, a New York City nutritionist and author of Cooking with Joy.
Weight training is the ultimate way to burn calories fast. "A pound of muscle burns up to nine times the calories of a pound of fat," explains Richard Cotton, M.A., chief exercise physiologist for myexerciseplan.com. Weight training increases your resting metabolic rate, which is the number of calories you burn while sitting on your butt. What's more, it gives your metabolism an added boost after you exercise: It remains in overdrive for up to two hours after the last bench press, according to a study published in Medicine & Science in Sports & Exercise. Strapped for time? Try these quick moves: squats, bench step-ups, lunges, push-ups, pull-ups and crunches. In a pinch, just do single sets of 10 for each exercise -- you'll get optimal results for the time invested.
Yeah, we just told you to pump iron, but you also need to eat it. "If you don't have enough of this mineral, your body can't get enough oxygen to your cells, which slows down your metabolism," explains Samantha Heller, R.D., a nutritionist at the New York University Medical Center. Most multivitamins contain around 18 mg (the RDA for adults); you can also get your fill by eating three to four daily servings of foods rich in iron, such as lean red meat, chicken, fortified cereal and soy nuts. If you are feeling symptoms such as fatigue and weakness, ask your doctor to test you for anemia (it's a simple blood test) at your next physical.
Read more: How to Burn Fat - 21 Ways to Burn Fat Faster - Redbook | <urn:uuid:a5c96db9-bb95-403d-8726-eff324341cc7> | CC-MAIN-2017-47 | http://workoutfatburner.blogspot.com/2013/04/21-ways-to-burn-fat-faster.html | 2017-11-22T20:25:10Z | s3://commoncrawl/crawl-data/CC-MAIN-2017-47/segments/1510934806660.82/warc/CC-MAIN-20171122194844-20171122214844-00678.warc.gz | en | 0.951378 | 630 | [
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How it Works
|Check The Availability. Enter your zip code and the move date to find available moving companies and moving help in your area.|
|Get Instant Moving Quotes. Instantly compare the moving prices side by side and obtain moving quote.|
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There is a lot of stress associated with the moving process. And if you have to move at the last minute, that just adds up to your stress. MoversCorp.com have created a guide to make your relocation happen successfully in a short period of time.
Short Notice Move
Many customers decide to move on their own, which includes packing, loading and driving a truck. This option allows you to choose how much help you need based on your preferences and budget.
When planning a local move, it is important to be prepared before your hired company arrives. Movers Corp provides you with all the moving suggestions and tips for a carefree relocation.
When moving there are so many things need to be done and one of them is to change your mailing address. You need to contact utility companies, banks, set up mail forwarding with the post office. Here is an article prepared by Movers Corp which will help you in this task.
Changing Your Address
When you are planning to move there are questions you need to ask your local movers and helpers to make sure that you are selecting the right people for your job.
Questions for Movers
Moving goes hand in hand with storage. But before you rent a storage unit, make sure to look into a respectable self storage company in your local area.
Self Storage Tips
Local moving companies and professional movers in Glencliff, 03238
Find the best local moving companies in Glencliff at MoversCorp.com. Whether it's a big or small move, simply enter your zip code to get access to cheap moving service providers in your local area. Our Glencliff movers provide professional moving and packing services, furniture and appliance moves, moving boxes and supplies as well as affordable moving labor services such as truck loading help and truck unloading help. Need help moving a house or an apartment, or help to load and unload a rental moving truck? Hire full-service movers and moving labor helpers in Glencliff, New Hampshire through our site. You will find professional movers and move help at discounted rates. They also provide in-home furniture moving service - from one room to another or from upstairs to downstairs. Moving labor service is ideal for people who move within the home or in the same apartment complex. And if you move yourself, you can hire movers to load and unload furniture at your destination at low cost. Each moving company in Glencliff, NH lists the services and equipment they provide. | <urn:uuid:c326836b-828c-4fe9-ba64-481105784e3e> | CC-MAIN-2017-47 | http://www.moverscorp.com/moving_companies/NH_New-Hampshire/Glencliff/ | 2017-11-23T15:20:30Z | s3://commoncrawl/crawl-data/CC-MAIN-2017-47/segments/1510934806842.71/warc/CC-MAIN-20171123142513-20171123162513-00678.warc.gz | en | 0.939514 | 590 | [
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We are members of our school’s Model United Nations, an international debate team, where we recently we won first place at the Naples Council on World Affairs for detailing a plan regarding the Syrian refugee crisis: purchasing and shipping bicycles to a refugee camp in Jordan currently housing over thirty thousand people. The plan would enable them to access school and provide an affordable and efficient method of transportation.
While born theoretical, the three of us–Daniela, Javier, and Stefania–decided to manifest this as close to reality as possible.
To launch our program, we are focused heavily on fundraising, such as organizing a walk/bike-a-thon in Cape Coral, Florida to collect donations to allocate towards a non-profit that would pay and ship bicycles to refugee camps. However, we ran into a problem.
While our original plan was dedicated towards Syrian refugees and their relief, we found that no organization was able to ship equipment to Middle Eastern nations. This was due to the fact that bike repair shops had to be built and the region was too insecure to send volunteers to fulfill positions as needed.
Currently, we are in partnership with Working Bikes, who is transforming our 20-page outline for a make-believe competition into a stroke of pure humanity in reality.
We are overwhelmingly passionate for the well-being and security of those experiencing barriers in their lives, particularly children who one day could grow-up to reconstruct their home country (an idea made possible with the transport access to education).
Hours upon hours have been devoted towards researching the lives, values, troubles, and weight carried on the global community. We have inspired forty committed International Baccalaureate (IB) students to collect donations for our cause.
The main event of the project is our Make a Difference Bike/Walk-a-thon scheduled for December 10th at Cape Coral High School. The date of our event will sit on one of the founders’ birthday, and as painfully cliche it is, she adores the fact that the celebration of her service project shares her turn into adulthood.
Our goal is to raise $2,000 to directly donate to Working Bikes. We have gained support from various local businesses and the Naples Council on World Affairs, which facilitated the original Model UN Syrian Refugee competition.
This project is an invaluable, incredible, and humbling opportunity to leave a small, but significant impact on those being delayed of their human rights. We await the success of empathy to come. We await another victory for kindness.
This post was written by Cape Coral High School students Daniela Barata, Javier Diaz, and Stefania Lugli. | <urn:uuid:50c812c5-615c-4db4-b7ab-8f2384a2429a> | CC-MAIN-2017-47 | https://www.workingbikes.org/making-a-global-impact-with-working-bikes/ | 2017-11-23T15:05:16Z | s3://commoncrawl/crawl-data/CC-MAIN-2017-47/segments/1510934806842.71/warc/CC-MAIN-20171123142513-20171123162513-00678.warc.gz | en | 0.969864 | 540 | [
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Contact this Business
Pierre's Courtside Cafe was founded in 2003, and is located at 2 Main St in New Britain. It employs -- employees and is generating approximately $0.00 in annual revenue. Additional information is available by contacting this business at (860) 832-8200.
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BUSINESS INFORMATION REPORT™
Receive a one-time comprehensive report on another company | <urn:uuid:102758fc-af79-4be2-9735-5dfb1b716a22> | CC-MAIN-2017-47 | https://credibility.com/cafeterias-grill-buffets-and-buffets/us-ct-new-britain/pierres-courtside-cafe | 2017-11-24T09:32:52Z | s3://commoncrawl/crawl-data/CC-MAIN-2017-47/segments/1510934807344.89/warc/CC-MAIN-20171124085059-20171124105059-00678.warc.gz | en | 0.935909 | 90 | [
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To properly experience our web site, you will need to make use of an alternative browser or upgrade to a newer variation of net Traveler (IE9 or higher). I'm going to have to develop this, I just got a MacBook Pro as well as its like having a jet engine on my desk. The High Rise Height Adjustable Laptop Stand with Storage space Cup creates a functional, standing work area for your laptop computer or tablet. I have not yet acquired a laptop stand, checked a couple that I did not such as and continued making use of a milk cart also. While work areas and your standard desk setups still exist, a growing number of employees are finding means to make the day-to-day of their careers healthier and much more comfy. This setup is suitable technology definition for kids (have a peek at these guys) viewing from numerous areas, or for installing the TV in the edge of a room to save room. I utilize it below my mixer and also have actually never ever had my laptop computer fall off, but the 90-degree angles, zip connection demand and overall wobbliness knock it down to three celebrities. I chose to get this stand after choosing it was simply too pricey to purchase or build my own stand desk.
We have a fresh batch of iPad Stands in. Our iPad stand is generally the exact same layout as our prominent apple iphone stand, simply 33% bigger. You could use very a lot anything to elevate your laptop computer screen to eye level if a laptop computer stand simply isn't really in your spending plan. Everyone has a different body, a various laptop computer, and also a different workdesk setup, obviously, so it's difficult to advise a single fixed-height laptop stand that will function for everybody. Due in August for $49.99, this basic, black stand provides a lot of capabilities that are unique in its group. Most preferred form of these gadgets is available in the form of a laptop computer holder for bed, yet there are many other forms, such as laptop table stand or flexible laptop holder for sofas. Bring your laptop computer over the rest of your devices and conserve that valuable table real-estate with this Laptop Mean DJs from Monoprice!
Absolutely nothing damages the look of a nice TV placed on the wall quicker compared to a tangle of revealed wires. He does tiling, window treatments, clog as well as leakage repair services, TELEVISION setup, and extra. Crane Hardware provides the best adjustable laptop computer depends on the marketplace, making use of premium workmanship and also lasting company techniques while keeping an extensive dedication to customer care. The screen installs at any of Field's pre-set elevations as well as integrated wire monitoring channels keep wires neatly out of sight.
After my surgical treatment I'll have to stand a lot, so I could see that I'll have the ability to use this after that also to keep my laptop computer at an economically proper level. Our laptop computer as well as tablet stands are light-weight as well as no larger than a tablet computer, particularly made to blend in conveniently with your various other mobile gadgets without bearing down your bag. Sporting a basic yet effective design, the Fellowes Smart Suites Laptop Riser places your note pad in a nearly upright position, with the trackpad protected behind a shield. Our TV setups are totally backed by a lifetime minimal handiwork service warranty that comforts you that our TELEVISION installation service will certainly constantly supply only ideal performance. You could constantly buy bigger washing machines if you accidentally make the openings as well huge so the bolts slip through.
You'll find a variety of TVs to cater to every watching choice, including 4K, OLED, LED and also plasma Televisions And we bring all the devices you should make your TV viewing area one-of-kind, from forecast display devices and also speakers to computer game gaming consoles as well as Blu-ray players.
Whether you're functioning from a standing workdesk or being in a rolling chair, having your computer boosted gives comfort and also relief to your neck and back, so you aren't left hunched over your key-board all the time. Lift complies with health and wellness requirements, making your laptop more secure and also more comfy to make use of all day long. I have discovered that there is no much better base on the marketplace compared to the mStand from Rain Design.
In order to help you out, we have actually put together a listing of specialist ideas in hopes of helping you avoid certain mistakes as well as conquer some of typical challenges provided when installing a TV. There is an on/off activate the follower & 3 USB Ports so you could power accessories throughout the stand. Think it or not, we discovered that this stand works as a rather huge heatsink, allowing your MacBook or MacBook Pro to stay cooler much longer. Their setup staff will help you in installing your TV, and also they also style systems that could fit into your budget plan.
Set up: The Uberstand takes the least time to establish as well as take down, while the L Stand M takes the longest. On the downside, expressing installs have the tendency to be a bit more considerable compared to flat wall installs. I have an Apple TELEVISION (not the newest sleek one) that now lives in a great owner, using hooks, on the rear of my Television Set. If you or your laptop are especially tall or brief, however, you'll be better off with a flexible stand. It places my Apple TELEVISION completely to the rear of my wall placed Samsung TV. You couldn't ask for a much better place. Nevertheless, considered that I utilize it standing, I have the separate keyboard stand which aids to reduce that issue (though not absolutely because it additionally wobbles a little bit). In addition to Apple TV 3 (2012), this TotalMount shelf likewise deals with Apple TV 2 (2010).
After that add thinner dowels put from listed below to create the legs (set to your needed elevation) and two each side from above at the front to be the front quits, and also one each side at the back to support the laptop computer. They could be among the most valuable devices in the box, or the reason you placed 10 holes in the wall without striking a stud. Lots of cabinet mounts could be tilted or rotated, making it very easy to locate the perfect viewing angle. Easy, effective, and very Mac-like design raises your laptop and also makes it more ergonomical for desktop usage, supplies cooling benefits too. Obtain your industrial TV install properly done by a company that would certainly not let you down and also understand the effects and also obligations of you doing this for your business. | <urn:uuid:350c351a-9fdd-49f3-abde-dac58673ff04> | CC-MAIN-2017-47 | http://wp.serov112.ru/tag/technology%20news%20ny%20times/ | 2017-11-25T03:46:54Z | s3://commoncrawl/crawl-data/CC-MAIN-2017-47/segments/1510934809392.94/warc/CC-MAIN-20171125032456-20171125052456-00678.warc.gz | en | 0.960426 | 1,373 | [
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Your Personal Horoscope -
With Cancer as your Sun Sign
The Sun is in the cardinal water sign of Cancer in your chart. With the Sun in this sign, you are very much someone who lives on your emotions. You are impressionable and sympathetic to others and able to listen patiently, but when your own feelings are hurt, you can be very moody and irritable. You tend to protect your feelings and emotions behind a hard exterior shell, just like a crab. Your family and roots are important to you, but you need to develop your own individuality. You will have a strong parental instinct and will be protective and supportive to your children. If you have no children, this will probably be expressed in some other area of your life. You can be very tenacious when pursuing your goals, or holding on to a relationship or other situation, perhaps too long at times. If this tenacity is carried to extremes, you may have a tendency to hang on to real or even imagined hurts.
With Cancer as your Ascendant
The cardinal water sign of Cancer was rising at the time of your birth, indicating that you are sensitive and reserved in your approach to others, although you may have learned at an early age to protect your sensitivity behind the hard outer shell of the crab. You have a naturally caring and sympathetic nature, and you like to take care of those who are close to you, you have an instinctive understanding of their emotional needs and you may see anyone or anything your protective circle as a threat. Cancer is a cardinal sign, so for anyone in that circle, you will give out great energy and bonhomie. That close circle may not be just family orientated, it can be vocationally focused too.
This same emotional sensitivity makes you vulnerable, however, since not everybody has the same consideration for your feelings as you have for theirs. You will tend to hide any hurt or emotional pain behind a hard exterior, and to approach situations indirectly rather than risk a direct confrontation. You may have a tendency to cling to any hurt or slight, whether real or imagined, and you will need to learn to accept your sensitive emotional nature and channel it in some positive direction. You are natural worriers and the slightest ailment will cause great alarm within you, which may cause even more psychological problems.
Those with Cancerian ascendants love their food and are often formidable cooks, and within partnerships Cancerian ascendants show much ambition for their partners, and may push them to reach higher and achieve more and more.
Cancer is ruled by the Moon, as the ruler of your Ascendant, this planet is of particular importance in your chart.
How are the planets affecting you right now?
Right now Mercury is in your house of friends & groups so now’s the time to talk with people about cooperative efforts and joint projects. You might plan a group trip or a social event with people you know? These people can also help you solve problems or highlight matters for you to consider. Venus is passing through your house of career and creative projects will attract you now and anything where you can let your talents speak out. Jupiter opposes though this week and this may make you lazy and not want to work hard at the moment. Mars is hidden away in your house of solitude and this can lead to irritation as everything you do or try to achieve may be frustrated in some way. Keep your cool and try not to get too frustrated. You may have to be quite unselfish at work for an over demanding boss or co worker. Try to take their demands in good faith as the likelihood is that the work does need to be done. The New Moon in Gemini on the 25th May may encourages you to take a quieter more backseat role to the tasks and commitments that you have to do. Best not to make a big show of your actions now as you won’t feel truly comfortable if you do, try to remain modest and unassuming. | <urn:uuid:d5131c71-f572-4a8c-9aab-a6599621c102> | CC-MAIN-2017-47 | http://solarisastrology.co.uk/cancer.html | 2017-11-18T06:01:38Z | s3://commoncrawl/crawl-data/CC-MAIN-2017-47/segments/1510934804666.54/warc/CC-MAIN-20171118055757-20171118075757-00078.warc.gz | en | 0.959535 | 881 | [
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