nopperl/pmc-image-text · Datasets at Hugging Face

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0.41412	1384ff1583f14cb6adafa46ef22df454	SEM images of (a) MoS2-only, (b) MoS2/GNS 9 : 1, (c) MoS2/GNS 8 : 2, and (d) MoS2/GNS 7 : 3.	PMC9053862	d0ra03539d-f3.jpg
0.499084	8ca7b3852220426b88d5fbe7289625eb	TEM images of (a and e) MoS2-only, (b and f) MoS2/GNS 9 : 1, (c and g) MoS2/GNS 8 : 2 and (d and h) MoS2/GNS 7 : 3.	PMC9053862	d0ra03539d-f4.jpg
0.416096	998f2490660c46f68ffa82df0c8375e4	(a) Cycle performances of MoS2/GNS electrodes at 200 mA g−1. Charge–discharge profiles of (b) MoS2-only, (c) MoS2/GNS 9 : 1, (d) MoS2/GNS 8 : 2, and (e) MoS2/GNS 7 : 3 measured at a current density of 200 mA g−1.	PMC9053862	d0ra03539d-f5.jpg
0.472086	8b33c16a524740219d99d86698d0c17e	CV curves of (a) MoS2-only, (b) MoS2/GNS 9 : 1, (c) MoS2/GNS 8 : 2, (d) MoS2/GNS 7 : 3 in the 1st, 3rd, 10th scans at a scan rate of 0.2 mV s−1 in the potential range of 0.0–3.0 V vs. Li/Li+.	PMC9053862	d0ra03539d-f6.jpg
0.413904	9e0bf31570624a0b9c3bd1db7f97daf1	High-rate performance of the MoS2/GNS anodes measured at varying current densities for each 10 cycles.	PMC9053862	d0ra03539d-f7.jpg
0.420623	f53f4338a0dd4651940a698ddd219195	Nyquist plots of the samples after 3 cycles at a current of 200 mA g−1.	PMC9053862	d0ra03539d-f8.jpg
0.423883	1a5c0f05f7594bdabd4d46d6fd8b0d82	The GITT analysis after the 3rd cycle. (a) Diffusivity of Li+ ion according to the state of charge (SOC) and (b) the average Li+ ion diffusion coefficients of the samples.	PMC9053862	d0ra03539d-f9.jpg
0.407229	89d9e601d6744fd0b68d655e34cfdf1f	Statistics of the main clinical manifestations of children.	PMC9054457	CMMI2022-1985531.001.jpg
0.440322	92ee31283e52419998307c2d4f69c3d2	CT image characteristics of children before and after treatment.	PMC9054457	CMMI2022-1985531.002.jpg
0.360993	f4695dab53bb43f4a41ec0abbc92fbb4	The main manifestations of chest HRCT in children with MPP in acute phase.	PMC9054457	CMMI2022-1985531.003.jpg
0.437115	2412622a178a4bbe92d06e9d38771b91	The correlation between different lung HRCT manifestations and PVF in children ( ∗(P) < 0.05).	PMC9054457	CMMI2022-1985531.004.jpg
0.485535	dea0e00e8858477f9daa5090854073c5	Comparison of PVF indicators in MMP children at different stages ( ∗(P) < 0.05).	PMC9054457	CMMI2022-1985531.005.jpg
0.436121	c231e6f3915f4d8081c42bdc092570bd	Flow diagram for the construction of CoRE MOF 2019 database.	PMC9055497	d0ra02498h-f1.jpg
0.402622	54170f77a07b4b1dbc8502cb7fab8da2	Examples of artifacts being screened in this paper. Panel (A) is an example of isolated atoms in the data that are likewise isolated in the real physical specimen (the circled atoms are F− ions). Panel (B) is an example of isolated atoms in the data that are likely not isolated in the real physical specimen (the circled atoms are oxygen atoms which likely belong to water molecules in the physical specimen for which hydrogen atoms were omitted in the reported crystal structure). Panel (C) is an example of overlapping atoms. Panel (D) is an example of misplaced hydrogens. Panel (E) is an example of under-bonded carbons. Panel (F) is an example of over-bonded carbons.	PMC9055497	d0ra02498h-f2.jpg
0.480474	f45fa410683c4bb984284849bd150ee1	Flow diagram of this project.	PMC9055497	d0ra02498h-f3.jpg
0.43588	b18eaa6eaf6f420d81982b4e7635adc7	Schematic illustration of borophene with few layers exfoliated by the probe ultrasonic assisted solvothermal treatment process.	PMC9055579	d0ra03492d-f1.jpg
0.44416	78ed905115f7488eb4acf6dff624342c	Optical microscope photos, SEM images, and TEM images of borophene with few layers exfoliated in different solvents: acetone (a–c), DMF (d–f), acetonitrile (g–i), ethanol (j–l), and NMP (m–o) (the insets show the Tyndall effect of borophene dispersion with few layers).	PMC9055579	d0ra03492d-f2.jpg
0.384438	de2238997e80422988fd8e2ceca0d51d	AFM images and the corresponding height profiles of borophene with few layers exfoliated in different solvents: acetone (a and b), DMF (c and d), acetonitrile (e and f), ethanol (g and h), and NMP (i and j), respectively.	PMC9055579	d0ra03492d-f3.jpg
0.414126	b721a50effe94f6a91273cac3cef82c0	Statistical data showing the lateral flake size average (a) and the thickness (b) of borophene with few layers in acetone.	PMC9055579	d0ra03492d-f4.jpg
0.423229	0fb06dc1bd09489abb165a277f305291	SEM images with low resolution (a) and high resolution (b) and TEM images of borophene with few layers obtained by probe ultrasonic assisted solvothermal exfoliation in acetone and corresponding FFT pattern of the selected area (c–e).	PMC9055579	d0ra03492d-f5.jpg
0.44754	9ba1792e2b7d48c78d4aa57af11e0e24	HAADF STEM and corresponding EELS elemental mapping images of borophene with few layer boron sheets by probe ultrasonic assisted solvothermal exfoliation, followed by centrifugation treatment in acetone: STEM image (a), boron (b), carbon (c), and oxygen (d).	PMC9055579	d0ra03492d-f6.jpg
0.41099	e14d8db427de4ff2aa9c9790301ec3e8	XRD patterns (a) and Raman spectra (b) of bulk B and borophene with few layers obtained by probe ultrasonic assisted solvothermal exfoliation.	PMC9055579	d0ra03492d-f7.jpg
0.402509	5f0ca5ddd95e4ae7a30e34d6fc9883bb	XPS spectra (survey) (a) and short scan XPS B 1s (b) of bulk B and borophene with few layers obtained by probe ultrasonic assisted solvothermal exfoliation.	PMC9055579	d0ra03492d-f8.jpg
0.410164	5a8e3deaef83423fa7e32fa38091ec15	(a) The high-resolution Co 2p, (b) Cu 2p, (c) C 1s, and (d) N 1s XPS spectra of the CD@CuCoPBA nanocomposite. (e and f) Low- and high-magnification SEM and (g and h) TEM images of the CD@CuCoPBA nanocomposite.	PMC9055645	d0ra01439g-f1.jpg
0.423299	cebd5be0385945608e2dcae813444b97	Cell viability of CD@CuCoPBA with different (a) CD@CuCoPBA concentrations and (b) MTT incubation time intervals, (c) cell uptake behaviors of CD@CuCoPBA incubated with MCF-7 cell for 1 h. Confocal microscopy images were obtained through different channels according to the CD@CuCoPBA emission (the scale bar corresponds to 50 μm).	PMC9055645	d0ra01439g-f2.jpg
0.452372	1f63a5f2fd9c41d6b07211df2dd51c3b	EIS Nyquist plots of the determination procedure for EGFR by using (a) the CuCoPBA- and (b) CD@CuCoPBA-based sensors, including the bare AE, materials/AE, Apt/materials/AE, and EGFR/Apt/materials/AE; (c) EIS responses of the Apt/CD@CuCoPBA/AE against EGFR with different concentrations of 0.001, 0.005, 0.05, 0.5, 5, 50, 500 and 1000 pg mL−1; (d) dependence of the ΔRct values on the EGFR concentration detected by the Apt/CD@CuCoPBA/AE. The linear part of the calibration curve is shown in the inset of (d). (e) The sensing selectivity measurement. The ΔRct values of the proposed electrochemical sensor by separately adding different interferents, including HER2, IgG, IgE, PSA, BSA, and CEA with the concentration of 0.5 pg mL−1, EGFR of 5.0 fg mL−1, and their mixture. (f) Reproducibility, (g) stability, and (h) regeneration of the proposed aptasensors for detecting EGFR with the concentration of 5.0 fg mL−1 (n = 3).	PMC9055645	d0ra01439g-f3.jpg
0.416736	978146b2d0c6437385c5c7e724f0a04d	(a) EIS Nyquist plots and (b) CV curves for testing the whole detection procedure of MCF-7 cells using the proposed CD@CuCoPBA-based aptasensor. (c) EIS responses of the Apt/CD@CuCoPBA/AE against MCF-7 cells with different concentrations of 5 × 102, 1 × 103, 5 × 103, 1 × 104, 5 × 104, and 1 × 105 cell per mL; (d) dependence of the ΔRct values on the MCF-7 cell concentration using the Apt/CD@CuCoPBA/AE. The linear parts of the calibration curves are shown in the inset of (d). (e) The ΔRct values of CD@CuCoPBA-based aptasensor for detecting L929 and C6 cells with the concentration of 500 cell per mL. (f) Reproducibility of the CD@CuCoPBA-based sensor for detecting MCF-7 cells with the concentration of 100 cell per mL (n = 3).	PMC9055645	d0ra01439g-f4.jpg
0.406426	4c8b22e538a24e019003231bec16acf7	Schematic working principle of the developed functional DNA biosensors that use SPMs A, B and C loaded with aptamers or DNAzymes and enzyme-coated SiO2 microbeads for read-by-eye distance-measuring quantification of adenosine, interferon-γ, and Pb2+ (as three model analytes) based on starch-hydrolysis-adjusted wettability change in strip-like μPADs, respectively.	PMC9055663	d0ra04619a-f1.jpg
0.42062	148d380c7f3c4203ae951792a6563583	Flowing distance (FD) results of the colored reagent obtained from different solutions in the strip-like μPADs. The scale bar is 3.5 mm.	PMC9055663	d0ra04619a-f2.jpg
0.395553	70b4d68b059a4a0f9c62be2ab396c72f	(A) Flowing distance (FD) results obtained from a starch solution (0.5 wt%), a blank sample (PBS buffer without the analyte), 50 μM adenosine, and three other sorts of small molecules (100 μM each). (B) Calibration curve that describes the relationships between the FD change (ΔFD = FDadenosine − FDblank) and the logarithm values of the adenosine concentrations (log[adenosine]) in the buffer samples. The linear regression equation is y = 2.7651x + 1.8551 (R = 0.9914). Each error bar represents a standard deviation across three replicate experiments.	PMC9055663	d0ra04619a-f3.jpg
0.399264	e59ea8a40bf341fe855e14398317427a	(A) Flowing distance (FD) results obtained from a blank sample (HEPES buffer without the analyte), 30 nM interferon-γ, and three other sorts of proteins (10 μM each). (B) Calibration curve that describes the relationships between the FD change (ΔFD = FDinterferon-γ − FDblank) and the logarithm values of the interferon-γ concentrations (log[interferon-γ]) in the buffer samples. The linear regression equation is y = 2.7406x + 2.6643 (R = 0.9961). Each error bar represents a standard deviation across three replicate experiments.	PMC9055663	d0ra04619a-f4.jpg
0.411336	1cb7f95ea06d4dec9af1042e71fd0170	(A) Flowing distance (FD) results obtained from a blank sample (HEPES buffer without the analyte), 50 nM Pb2+, and twelve other sorts of metal ions (1 mM each). (B) Calibration curve that describes the relationships between the FD change (ΔFD = FDPb2+ − FDblank) and the logarithm values of the Pb2+ concentrations (log[Pb2+]) in the buffer samples. The linear regression equation is y = 2.7252x + 2.3799 (R = 0.9973). Each error bar represents a standard deviation across three replicate experiments.	PMC9055663	d0ra04619a-f5.jpg
0.431841	f140e3d9ff564148ba070acbf4931b47	(a) Ball and stick models of IL ions and peptide dimer. (b) Simulation boxes of peptide dimer in 10% and 70% (w/w) EAM, EAN, and TEAM ILs. Color scheme: cation-transparent surface, anion, water-stick, peptide-new cartoon.	PMC9056671	d0ra06609e-f1.jpg
0.434459	149699aff5b1490593450c6455ab72de	(a) Free energy profile of peptide (16–22) dimer in water and corresponding conformations of minima. (b) RDFs of water hydrogen and oxygen atoms around amide oxygen and hydrogen atoms of the peptide backbone. The dashed lines represent the number integral for the corresponding RDF.	PMC9056671	d0ra06609e-f2.jpg
0.436529	d9601f46ae3d4d26864a8ff5c7d572d3	Free energy profiles of peptide (16–22) dimer in 10% and 70% (w/w) EAM (a and b), EAN (c and d), and TEAM (e and f) ILs and corresponding conformations of minima.	PMC9056671	d0ra06609e-f3.jpg
0.441426	0f0901c59df8456badfe7cf8a385dad2	Semi-log representation of RDFs of cation, anion, and water around amide oxygen and hydrogen atoms of the peptide backbone for the corresponding minimum free energy conformations in 10% (w/w) EAM (a and b), EAN (c and d), and TEAM (e and f) IL. The dashed lines represent the number integral for the corresponding RDF.	PMC9056671	d0ra06609e-f4.jpg
0.481168	b1363b102add47aa9a848c33bfe81e55	Semi-log representation of RDFs of cation, anion, and water around amide oxygen and hydrogen atoms of the peptide backbone for the corresponding minimum free energy conformations in 70% (w/w) EAM (a and b), EAN (c and d), and TEAM (e and f) IL. The dashed lines represent the number integral for the corresponding RDF.	PMC9056671	d0ra06609e-f5.jpg
0.477216	9746d28b91ff42ec93b11ec4dc52899f	The average solvent-accessible surface area of the peptide dimer overall solutions with changing the average Ψ.	PMC9056671	d0ra06609e-f6.jpg
0.479917	b0db83271924414aab6544946b551252	Comparative surface plasmon resonance peaks of reaction mixtures for AgNPs synthesis in WPE, and extracts from TDZ-induced cell cultures, and MLN-induced cells cultures. Controls; cell extracts only, Ag, and WPE only.	PMC9057356	d0ra08419k-f1.jpg
0.469414	20f89becf2f9402695497250e757718d	Dose–response recorded in terms of AgNPs yield when supplemented with different concentrations of WPE and extracts from TDZ-induced cell cultures, and MLN-induced cell cultures at a constant concentration of AgNO3 (2 mM).	PMC9057356	d0ra08419k-f2.jpg
0.454465	cada31a3d0c74d42b49ef73c44001d9c	Time course analysis of yield of AgNPs biosynthesized through extracts of TDZ-induced cell cultures, MLN-induced cell cultures, and whole plant of F. indica.	PMC9057356	d0ra08419k-f3.jpg
0.408883	c0b48e76a6194a4d8c1d556ebc4f242c	Comparison of the reaction mixture of AgNPs synthesized through extracts of MLN induced cell cultures (left), TDZ-induced cell culture (center), and whole plant (right) after 48 hours of incubation.	PMC9057356	d0ra08419k-f4.jpg
0.528795	d28e47c1647c47e2b30714af731eed05	Transmission electron microscopy (TEM) based images of AgNPs synthesized via extracts from (a) MLN-supplemented cell cultures (b) TDZ-supplemented cell cultures and (c) naturally grown F. indica.	PMC9057356	d0ra08419k-f5.jpg
0.459738	bf7002e9d7174874801c4eacf3835d56	Size distribution of AgNPs synthesized through aqueous extracts of (a) TDZ-induced cell cultures (b) MLN-induced cell cultures and (c) naturally grown F. indica.	PMC9057356	d0ra08419k-f6.jpg
0.603377	0fa05fc72ef64569b32881b0e83498e1	Venn diagram for the comparative analysis of compounds detected through LC-MS/MS.	PMC9057356	d0ra08419k-f7.jpg
0.549445	b02e05bbbecf47fa937958994b9e65d0	Reactive oxygen quantification in bacterial cultures grown in the presence of different concentrations of AgNPs synthesized in MLN-based in vitro callus cultures.	PMC9057356	d0ra08419k-f8.jpg
0.441635	8706347f48a44fbf9fda1e8d0ed942a3	The topographic distribution of the pelvic resection zones.	PMC9057553	JDRS-2022-33-1-117-131-F1.jpg
0.414584	0424858b25904de089766fb363b0f704	Extended lateral incision with the flexible lateral decubitus position.	PMC9057553	JDRS-2022-33-1-117-131-F2.jpg
0.423293	9de788037d024365a9aa8f12c6943130	(a) Preoperative anteroposterior (AP) pelvis radiograph of the 42-year-old patient with recurrent malignant giant cell tumor (mGCT) involving almost the entire left hemipelvis. (b) 3-dimensional (3D) reconstruction on computed tomography (CT) with the soft tissue component of the recurrent tumor involving the left hemipelvis of the same patient. (c) 3D reconstruction on CT with only bony structures revealed the massive destruction, particularly in zone II and zone III. (d) Postoperative AP pelvis radiograph at 10 years. (e) 3D CT AP view of the patient who underwent combination of allograft and cemented arthroplasty after Zone I + II + III resection. (f) 3D CT lateral view of the patient who underwent combination of allograft and cemented arthroplasty after Zone I + II + III resection. (g) Excellent functional result was obtained with 80% musculoskeletal tumor society (MSTS) score.	PMC9057553	JDRS-2022-33-1-117-131-F3.jpg
0.419355	cdeaee192355473aba7995d1a51a610e	(a) Preoperative axial computed tomography (CT) image of our 45-year-old patient with right periacetabular osteogenic sarcoma (OGS). (b) Intramedullary OGS in the right periacetabular region in coronal T2 contrast-enhanced magnetic resonance images (MRI). (c) Postoperative anteroposterior (AP) pelvis radiograph at three years. (d) Aseptic loosening complication at the allograft and cement interface of hip arthroplasty in the 44th month follow-up of our patient who underwent type I + II resection. (e) Postoperative 10th year control AP pelvis radiography after revision with acetabular cage + cemented constraint cup. (f) Excellent functional result was obtained with 85% musculoskeletal tumor society (MSTS) score. He walks without any assistive device.	PMC9057553	JDRS-2022-33-1-117-131-F4.jpg
0.393847	1edd14613f69487cbb281e2b0e43a3dd	(a) Preoperative anteroposterior (AP) pelvis radiograph of the 35-year-old patient with high grade chondrosarcoma (CHS) involving right hemipelvis. (b) Intraosseous chondroid matrix mineralization and cortical destruction are seen in axial T2 contrast-enhanced magnetic resonance images (MRI) in right ileum. (c) Postoperative 6th year AP pelvis radiography. Excellent functional result was obtained with 80% musculoskeletal tumor society (MSTS) score. She walks without any assistive device. (d) It was possible to remove the tumor en bloc by performing wide resection with the surrounding gluteus maximus and iliacus muscle. (e) Allograft was placed anatomically between the pubic, ischium and residual crescent bone fragments, and the graft was supported vertically with lumbopelvic stabilization in addition to the combination of cemented constraint hip arthroplasty.	PMC9057553	JDRS-2022-33-1-117-131-F5.jpg
0.434124	7e161c4add1d4d359d810c0131177e4a	(a) Preoperative anteroposterior (AP) pelvis radiograph of the 43-year-old patient with giant high grade MMT causing destruction of the right hemipelvis (b) Coronal CT images showed how much the soft tissue component pushes the visceral organs to the contralateral side, and destruction of the tumor, especially in the periacetabular area and ischium. (c) Considering that the large tumor even crossed the midline medially, only marginal resection could be made, as can be understood from the specimen. (d) Osteosynthesis with reconstruction plates between the residual ileum and pubic bone and the perfectly cut allograft. Hip joint restoration with large-head proximal femur resection prosthesis for stability. (e) Postoperative 5th months AP pelvis radiography. This patient died of lung metastasis with deep infection before local recurrence was observed in the 6th postoperative month. Poor functional result with the MSTS score of 26% and could only be mobilized with 2 crutches.	PMC9057553	JDRS-2022-33-1-117-131-F6.jpg
0.40724	bab24d4f62634a8681a0fd0a57dc66f6	Patient flow chart. PH, pulmonary hypertension.	PMC9058072	fcvm-09-814557-g001.jpg
0.438899	bd391e8d9e3f4d2ea0b386f8805d2f1b	The relative feature importance of predictor variables included in the gradient boosting decision tree for predicting the death of pregnant women with pulmonary hypertension. NT-proBNP, N-terminal brain natriuretic peptide; PASP, pulmonary artery systolic pressure; ALB, albumin; RA, right atrium; APTT, activated partial thromboplastin time; RDW, red cell distribution width; PT, prothrombin time; WHO, World Health Organization.	PMC9058072	fcvm-09-814557-g002.jpg
0.411201	a58daaabbe3246cb811f786f102595da	The ROC curves of significant predictors. NT-proBNP, N-terminal brain natriuretic peptide; PASP, pulmonary artery systolic pressure; ALB, albumin.	PMC9058072	fcvm-09-814557-g003.jpg
0.541752	46bb342164e2476799861e9a7efdfc69	Schematic figure of the (a) Model A and (b) Model B versions of vertical diffusion cell.	PMC9058821	gr1.jpg
0.491779	90c96a6766bc4a9b8f6902a41d7191b1	Schematic illustration for comparison of (a) Franz cell apparatus and (b) the novel modified vertical diffusion cell apparatus.	PMC9058821	gr2.jpg
0.468305	f21a1d3b4d79418a93216eca5ab96edb	Three-dimensional views of various constructions of modified vertical diffusion cell ((a) Model A, (c) Model B) and technical drawings of these devices ((b) Model A, (d) Model B).	PMC9058821	gr3.jpg
0.460014	ef17522746bb49cab68b77f472b9568d	Photo about ‘Model A’ version of the modified vertical diffusion cell. The inset shows in more detail what is the right combination of the donor (A1) and the acceptor (D8) compartments. The figure also shows the essential parts of cell: donor chamber (A), membrane (B1), sample (B2), silicone insert (B3), glass plate or cover plate (B4) stainless steel ball joint clip (C), acceptor chamber (D1), sampling inlet (D2), second overflow inlet (D3), second inlet tap (D4), first inlet (D5), first inlet tap (D6), ground inlet (D7), spherical edge of the acceptor chamber (D8), magnetic stirrer bar (E), Hamilton syringe (F), drip funnel (buffer tank) (G1), glass tap (G2).	PMC9058821	gr4.jpg
0.428323	2ea573814e234956b2ef68bf1fc40ca8	Schematic figure of operational system which contains the necessary main background instruments: double-walled vessel (H), sealed cap (I), temperature control thermocouple (O1), heated magnetic stirrer (O2), primary tempering fluid in baker, peristaltic pump (O3).	PMC9058821	gr5.jpg
0.464202	9dc4c1791cb749ca95512fbbae9101d8	Bland-Altman plot for inter-rater reliability analysis of each acetabulum location. The limits of agreement are shown as dotted, black lines with 95% confidence intervals (green and red areas), bias (as dotted black line) with 95% confidence intervals (blue area), and regression fit of the differences of the means (as solid black line). (a) Medial wall. (b) Posterior wall. (c) Superior wall. (d) Anterior wall.	PMC9059077	fx1.jpg
0.417251	d73eb2dad8804428ae9a49b2f9fda852	Postoperative coronal computed tomography image of the hip joint. The axial image was sliced at the center of the head.	PMC9059077	gr1.jpg
0.416695	1cf903fe1e9e434f811dc0363ee4e4c5	Preoperative axial computed tomography image of the hip joint. Image is at the same level as the postoperative image and sliced at the center of the head. Anterior, posterior, and medial walls were measured with the maximum elliptic mean value and did not include the cortex. The measurement of the medial wall was taken at the bisector of the anterior-posterior diameter of the acetabulum without a double floor.	PMC9059077	gr2.jpg
0.428385	e3e531ed23174c5a9c3eda0ec7a21b26	Maximum elliptic mean. In the superior wall, the coronal image at the center of the medial wall was used to measure the maximum elliptic mean without the cortical bone.	PMC9059077	gr3.jpg
0.458431	f649cca14cb34207bd431dc488fb19b3	Scatter plot of reliability at each region of the acetabulum. The plot shows inter-rater reliability assessed at the (a) superior, (b) anterior, (c) posterior, and (d) medial walls.	PMC9059077	gr4.jpg
0.431432	3f866fd5f6994048817bff0384b40828	Tripodal molecules 1a–4b.	PMC9059549	c8ra06647g-f1.jpg
0.476602	69fd53d9ff65462ca66fe57d490de548	Partial 1H NMR spectra of L1, 1a and 1b in d6-DMSO solvent.	PMC9059549	c8ra06647g-f2.jpg
0.416177	a4e780319ba14eac87c73ef6abda5954	The relative 1H-NMR chemical shift (in ppm) of H4–H7 protons (Mol = 1a–4b and L = L1–L4).	PMC9059549	c8ra06647g-f3.jpg
0.479801	8e8de04356c84879a0129e9ef2967212	Molecular structures of 1a, 1b, 2a and 2b evaluated at MPW1PW91/6-311+G(d,p) level of theory and basis function showing the cyclic benzene trimer (CBT) between three benzene rings of benzimidazolyl units.	PMC9059549	c8ra06647g-f4.jpg
0.422471	f72f68a76f5a40bb935925f50e1e3d83	MESP topography distribution of 1a, 1b, 2a and 2b evaluated at MPW1PW91/6-311+G(d,p) level of theory and basis set. The consecutive contours are separated by 0.02 a.u.	PMC9059549	c8ra06647g-f5.jpg
0.399157	9939cfcde7f24c08a92cd53c47dd3c27	The substitutions of nonstructural proteins during the five waves of SARS-CoV2 pandemic in Iran.	PMC9060343	pone.0267847.g001.jpg
0.429616	9f9de0d38ceb4c2cab084317e0c80f1f	Phylogenetic tree of SARS-CoV2 full-length genomes constructed by MEGA 7.The Neighbor joining method was used with 1,000 bootstrap replicates. The tree contains 54 SARS-CoV2 sequences of these study compared to the reference sequence from GISAID and some other sequences from each clade. In this tree the reference sequence is marked by white circle and sequences of this study were marked as follow: The 1st wave black circle, the 2nd wave inverted black triangle, the 3rd wave with black square, the 4th wave with black triangle and the 5th wave with black diamond.	PMC9060343	pone.0267847.g002.jpg
0.433668	afd72e90c6354a07a199feb533a1cf3c	Synthesis of a series of 1,2-bis(N-alkylimidazolium)ethane BF4− salts.	PMC9060431	c8ra09208g-f1.jpg
0.445908	0beab14082b54dea81d3a5fac4ba840b	500 MHz 1H NMR spectrum of C8 in acetone-d6.	PMC9060431	c8ra09208g-f2.jpg
0.539057	0ca8311c85c74edead749abd98d1e431	Relationship between the side-chain length and melting temperature/first solid–solid phase transition temperature for the synthesized dicationic imidazolium BF4− salts.	PMC9060431	c8ra09208g-f3.jpg
0.480821	b66546a5bd624663a80860c266505415	DSC thermograms of C6 (blue) and C7 (red) (2nd heating scans, heating rate = 10 K min−1, N2).	PMC9060431	c8ra09208g-f4.jpg
0.449368	46a8f762fc024c4abb4e4b60e9aa1570	DSC thermograms of C11 (blue) and C12 (red) (2nd heating scans, heating rate = 10 K min−1, N2). The small Tm peaks of C11 and C12 correspond to the small ΔSf values: 18 and 17 J K−1 mol−1, respectively.	PMC9060431	c8ra09208g-f5.jpg
0.390002	a355f0cad15d46f69613daf5239cff6c	POM images of (a–d) C6 and (e–h) C7 during cooling from their isotropic phases: (a) plastic crystal phase at 100 °C, (b) dark domains aligned at 80 °C before the solid–solid phase transition, (c) plastic crystal phase cooled to room temperature, and (d) crystalline texture after slow crystallization at room temperature. For C7: (e) plastic crystal phase at 150 °C, (f) dark domains aligned at 100 °C, (g) undulated pattern in the lower phase at 50 °C, and (h) cracked crystalline texture at room temperature. All scale bars correspond to 200 μm.	PMC9060431	c8ra09208g-f6.jpg
0.423126	de07b3807614413586573521d526997f	POM images of C12 during cooling from its isotropic phase: (a) at 200 °C, (b) at 150 °C, (c) at room temperature. All scale bars correspond to 200 μm.	PMC9060431	c8ra09208g-f7.jpg
0.433119	ad5236417fd043cbb6c46ba423a53aaa	1D wide-angle X-ray scattering spectra of (a) the plastic crystal C7 and (b) the liquid crystal C12 at different temperatures.	PMC9060431	c8ra09208g-f8.jpg
0.45312	8fae54621ee24f3ab9a36c164a251c43	Ionic conductivity (left axis) as a function of temperature while cooling combined with DSC cooling traces (right axis) for (a) C6 and (b) C11.	PMC9060431	c8ra09208g-f9.jpg
0.527817	300ab1a0dc664fef90b2cc1ffb663b3d	Comparison of patient satisfaction between the two groups.	PMC9060996	CMMM2022-4820090.001.jpg
0.494647	fdca8e2cb63e48cfbed5ea6667340b87	The concentric cell (BLM) for transport experiments (a relatively thick layer of immiscible fluid is used to separate the source and receiving phases).	PMC9062073	c8ra09118h-f1.jpg
0.5735	b4833f0594834716b8d6f58d861ea8be	Displacement ellipsoid plot (50% probability) and atom-numbering scheme for PTC. The H atoms are drawn as small spheres of arbitrary radii. Selected bond lengths (Å) are P1–O1 = 1.4718(16), P1–N1 = 1.7306(19), P1–N2 = 1.616(2) and P1–N3 = 1.615(2), and selected bond angles (°) are O1–P1–N1 = 103.29(9), O1–P1–N2 = 116.39(9), O1–P1–N3 = 116.53(10), N1–P1–N2 = 109.35(10), N1–P1–N3 = 107.28(10) and N2–P1–N3 = 103.66(10).	PMC9062073	c8ra09118h-f2.jpg
0.438626	54e07f502b984f6184dc0bcb37881f08	A view of one-dimensional array of molecules, built from NH⋯O hydrogen bonds. The weakly occupied atoms and hydrogen atoms not involved in hydrogen bonding were omitted for the sake of clarity.	PMC9062073	c8ra09118h-f3.jpg
0.496434	5eca9c6aca344ac6af52cd6b7aee0976	The possible tautomeric equilibrium in solution.	PMC9062073	c8ra09118h-f4.jpg
0.441593	b92bc9e424f34691a36c5ec2bd3e3911	Transport percentages for seven metal cations by PTC carrier in different organic solvents.	PMC9062073	c8ra09118h-f5.jpg
0.397876	536bbc4935e54c5092bd34cc06472a27	Molar conductance–mole ratio plots for the PTC–Cu(ii) complex in NB at different temperatures.	PMC9062073	c8ra09118h-f6.jpg
0.453798	539246d95c9d4e8d9864826dac44f6b0	Profiling gene expression and genomic accessibility in the plastic and fate-restricted RPE. The developing (dev.) eye at E4 (A) and posterior eye at E5 (B) are shown with RPE and neuroepithelium (NE; presumptive neural retina) labeled. A regenerating NE is observed 3 days PR if treated with FGF2 at E4 (C), but no regeneration is observed 3 days PR and FGF2 treatment at E5 (D). At 3 days PR, no regeneration is observed if surgery is performed at E4 (E) or E5 (F) in the absence of FGF2. Scale bars in (A–F) are each 200 μm. Asterisk (*) denotes the FGF2 bead. (G) Schematic summarizes the collection of RNA-seq and ATAC-seq samples used in this study. (H) Principal component analysis summarizes the variation present in RNA-seq normalized gene expression values across 18 samples and six tested conditions. (I) Heatmap displays blind hierarchical clustering of normalized ATAC-seq peak signal for each sample.	PMC9062105	fcell-10-875155-g001.jpg
0.460296	4063b66cd12a4283b9a34c82f4a5cfd3	Clustering of differentially expressed genes reveals expression programs associated with RPE commitment and reprogramming. (A) Affinity propagation clustering was performed on all genes that are differentially expressed across the E4 to E5 window by the criteria \|LFC\| ≥ 1 and adj. p-value ≤ 0.05 (n = 2551), resulting in six clusters. Key genes are summarized on the right of heatmap. The heatmap intensity displays row-normalized gene counts. (B) Bubble charts display select gene ontology terms associated with each cluster. Adj. p-value is plotted on y-axis and the bubble size is proportional to number of terms identified in the analysis.	PMC9062105	fcell-10-875155-g002.jpg
0.497795	1972eeb4e5d245c59efa71a03dacbc91	Neural retina identity genes are active 6hPR + FGF2 at E4 and E5. Genome browser views display the VSX2 (A) and SIX6 (B) loci, with the normalized counts for ATAC-seq and RNA-seq assays summarized in the coverage tracks above. The highlighted pink area represents changes in promoter-region accessibility. Normalized gene expression counts are displayed for neural retina-associated genes PAX6 (C) LHX2 (D) and ASCL1 (E). The y-axes are log-transformed. Asterisk () denotes an Adj. p-value ≤ 0.05. n.s., not significant. (F) Two representative RPE explants are shown after 48 h in culture in the presence of FGF2 at E4 (left) or E5 (right). Red arrows point to RPE-derived neural retina from E4 explants. (G) Gene expression was measured via RT-qPCR using explants cultured in the presence of FGF2 at E4 or E5 and collected at the specified time points. Bar chart displays the average expression, and the error bars represent standard error of the treatment mean based on ANOVA. Each dataset is normalized to basal levels observed at time 0; y-axes are log2-transformed. qPCR significance: n.s. denotes not significant, denotes p-value < 0.05, denotes p-value < 0.01, * denotes p-value < 0.001.	PMC9062105	fcell-10-875155-g003.jpg
0.490809	88c6dfe2d7974381ba8692be9f8bf51f	Proliferation is tightly regulated across the window of RPE cell fate restriction. (A) EdU and DAPI fluorescence staining of embryo sections collected 24 h PR (24hPR) and FGF2 treatment was performed at either E4 (A) or E5 (B). Fluorescence channels are overlayed with differential interference contrast (DIC) image. 50 μm scale bar in (A) applies to both panels. Chart displays the proportion of EdU positive RPE cells observed 24hPR + FGF2 at E4 and E5 (C). Diamond represents mean proportion; asterisk () denotes Adj. p value <0.05. (D) Gene set enrichment analysis (GSEA) was performed on normalized RNA-seq expression values from 6hPR + FGF2 E4 and E5 samples. A low normalized enrichment score (NES = −3.4349458) for E2F targets indicates high enrichment in the E4 6hPR + FGF2 RPE and depletion from the E5 6hPR + FGF2 RPE. Each black vertical bar represents a gene in the pathway. (E) The row-normalized heatmap displays RNA-seq expression values for E2F targets genes and proliferation-associated factors. (F) RT-qPCR was used to measure E2F1 expression in E4 and E5 explants cultured in the presence of FGF2 and collected at the specified time points. Bar chart displays the average expression value, and the error bars represent standard error of the treatment mean based on ANOVA. Each dataset is normalized to basal levels observed at time 0; y-axes are log2-transformed. qPCR significance: n.s. denotes not significant, denotes p-value < 0.05, denotes p-value < 0.01, * denotes p-value < 0.001.	PMC9062105	fcell-10-875155-g004.jpg
0.421058	00af08b26d5f446394ab790099b27fad	Gene signatures associated with RPE maturation are broadly elevated in the E5 RPE. Normalized RNA-seq expression values are displayed for genes encoding RPE-associated transcription factors (A), the ion channel BEST1 (B), retinol metabolism and transport factors (C), melanin synthesis machinery (D), and melanosome-associated proteins (E). For (A–E), asterisk () denotes an Adj. p-value ≤ 0.05 and n.s., not significant. Relative gene expression was measured in RPE explants using RT-qPCR, and is displayed for OTX2 (F), MITF (G), RPE65 (H), and TYR (I). Bar chart displays the average expression, and the error bars represent standard error of the treatment mean based on ANOVA. Each dataset is normalized to basal levels observed at time 0; y-axes are log2-transformed. qPCR significance: n.s. denotes not significant, denotes p-value < 0.05, denotes p-value < 0.01, * denotes p-value < 0.001.	PMC9062105	fcell-10-875155-g005.jpg
0.433335	2838d38512364273ad5b292bb2ec0a42	RPE maturation is accompanied by differential accessibility at retinal development genes and homeobox transcription factor binding sites. (A) Venn diagram summarizes the overlap of up-regulated DARs (DARs with increased E5 accessibility) between the developing and 6hPR + FGF2 conditions, defined by a cut-off of Adj. p-value ≤ 0.05. (B) Similarly, Venn diagram displays down-regulated DARs (E4-enriched DARs) for the two conditions. DARs unique to either condition were annotated to the nearest gene TSS within 10 kilobases, and pathway enrichment analysis was performed for DARs enriched in the developing E5 RPE (C), the E5 6hPR + FGF2 RPE (D), the developing E4 RPE (E), or the E4 6hPR + FGF2 RPE (F). (G) The log fold change (LFC) of differentially accessible peak summits between the E4 and E5 conditions are plotted. Dashed lines represent a LFC cut-off of 1 that was used to select summits for motif analysis. HOMER motif detection software was used to identify transcription factor DNA motifs overrepresented in the summits of peaks differentially accessible across the developing (H) or 6hPR + FGF2 (I) RPE, and the top 15 enriched motifs are shown.	PMC9062105	fcell-10-875155-g006.jpg
0.485006	f82ce83f9e6642e98e2ab296416d89a4	Overrepresented motifs in open regions of the E4/E5 RPE. The monaLisa motif analysis software was used to bin differentially accessible summits by accessibility across the E4 and E5 conditions. Each bin was analyzed for the overrepresentation of transcription factor motifs found in the JASPAR 2022 database. Enriched motifs identified at −log(p-Adj.) >3 in any bin were recorded and select factors associated with the developing RPE (A) or 6hPR + FGF2 (B) samples were plotted. (C) Genome browser displays ATAC-signal proximal to DARs containing the OTX2 binding motif. The region of interest is highlighted in pink and associated motifs are summarized below the track.	PMC9062105	fcell-10-875155-g007.jpg
0.383984	5da69b21807e42b88f0eb4319a14c9d8	Final thematic map.	PMC9062181	fpsyg-13-859240-g001.jpg
0.42157	ddc7295af2b746b9bf41325f5a8a687e	Phylogenetic tree showing the relationship among nitrogen fixing, phosphorus and potassium solubilizing bacterial isolates, 16S rRNA gene sequences with reference sequences obtained through BLAST analysis. The trees were constructed using neighbor joining (NJ) with algorithm using MEGA 4 software (Tamura et al., 2007).	PMC9062246	gr1.jpg
0.417003	bc8f86e5b40c47b6a032e70a15161944	Study design, patient selection and evaluation. ICU, intensive care unit.	PMC9062462	bmjopen-2021-051971f01.jpg
0.361355	1f4196bee0b2413e9a4ee869b04eadbb	Results of global coagulation tests (gb and hSonoclot) in the low flow (N=11), high flow (n=34) and invasive ventilation (n=29) oxygen requiring subgroups. Presence of heparin-like effect (HLE) as defined as difference in Sonoclot trace at days 1, 3 in patients. The prevalence of HLE and changes in Sonoclot test was assessed as per oxygen requirement and survival (A–F).	PMC9062462	bmjopen-2021-051971f02.jpg
0.376933	3dbb78a4feaf4c92a91a6e774b99b2b0	Kaplan-Meir survival curves for patients (A—oxygen groups; B—presence of heparin-like effect).	PMC9062462	bmjopen-2021-051971f03.jpg

0.41412

1384ff1583f14cb6adafa46ef22df454

SEM images of (a) MoS2-only, (b) MoS2/GNS 9 : 1, (c) MoS2/GNS 8 : 2, and (d) MoS2/GNS 7 : 3.

PMC9053862

d0ra03539d-f3.jpg

0.499084

8ca7b3852220426b88d5fbe7289625eb

TEM images of (a and e) MoS2-only, (b and f) MoS2/GNS 9 : 1, (c and g) MoS2/GNS 8 : 2 and (d and h) MoS2/GNS 7 : 3.

PMC9053862

d0ra03539d-f4.jpg

0.416096

998f2490660c46f68ffa82df0c8375e4

(a) Cycle performances of MoS2/GNS electrodes at 200 mA g−1. Charge–discharge profiles of (b) MoS2-only, (c) MoS2/GNS 9 : 1, (d) MoS2/GNS 8 : 2, and (e) MoS2/GNS 7 : 3 measured at a current density of 200 mA g−1.

PMC9053862

d0ra03539d-f5.jpg

0.472086

8b33c16a524740219d99d86698d0c17e

CV curves of (a) MoS2-only, (b) MoS2/GNS 9 : 1, (c) MoS2/GNS 8 : 2, (d) MoS2/GNS 7 : 3 in the 1st, 3rd, 10th scans at a scan rate of 0.2 mV s−1 in the potential range of 0.0–3.0 V vs. Li/Li+.

PMC9053862

d0ra03539d-f6.jpg

0.413904

9e0bf31570624a0b9c3bd1db7f97daf1

High-rate performance of the MoS2/GNS anodes measured at varying current densities for each 10 cycles.

PMC9053862

d0ra03539d-f7.jpg

0.420623

f53f4338a0dd4651940a698ddd219195

Nyquist plots of the samples after 3 cycles at a current of 200 mA g−1.

PMC9053862

d0ra03539d-f8.jpg

0.423883

1a5c0f05f7594bdabd4d46d6fd8b0d82

The GITT analysis after the 3rd cycle. (a) Diffusivity of Li+ ion according to the state of charge (SOC) and (b) the average Li+ ion diffusion coefficients of the samples.

PMC9053862

d0ra03539d-f9.jpg

0.407229

89d9e601d6744fd0b68d655e34cfdf1f

Statistics of the main clinical manifestations of children.

PMC9054457

CMMI2022-1985531.001.jpg

0.440322

92ee31283e52419998307c2d4f69c3d2

CT image characteristics of children before and after treatment.

PMC9054457

CMMI2022-1985531.002.jpg

0.360993

f4695dab53bb43f4a41ec0abbc92fbb4

The main manifestations of chest HRCT in children with MPP in acute phase.

PMC9054457

CMMI2022-1985531.003.jpg

0.437115

2412622a178a4bbe92d06e9d38771b91

The correlation between different lung HRCT manifestations and PVF in children ( ∗(P) < 0.05).

PMC9054457

CMMI2022-1985531.004.jpg

0.485535

dea0e00e8858477f9daa5090854073c5

Comparison of PVF indicators in MMP children at different stages ( ∗(P) < 0.05).

PMC9054457

CMMI2022-1985531.005.jpg

0.436121

c231e6f3915f4d8081c42bdc092570bd

Flow diagram for the construction of CoRE MOF 2019 database.

PMC9055497

d0ra02498h-f1.jpg

0.402622

54170f77a07b4b1dbc8502cb7fab8da2

Examples of artifacts being screened in this paper. Panel (A) is an example of isolated atoms in the data that are likewise isolated in the real physical specimen (the circled atoms are F− ions). Panel (B) is an example of isolated atoms in the data that are likely not isolated in the real physical specimen (the circled atoms are oxygen atoms which likely belong to water molecules in the physical specimen for which hydrogen atoms were omitted in the reported crystal structure). Panel (C) is an example of overlapping atoms. Panel (D) is an example of misplaced hydrogens. Panel (E) is an example of under-bonded carbons. Panel (F) is an example of over-bonded carbons.

PMC9055497

d0ra02498h-f2.jpg

0.480474

f45fa410683c4bb984284849bd150ee1

Flow diagram of this project.

PMC9055497

d0ra02498h-f3.jpg

0.43588

b18eaa6eaf6f420d81982b4e7635adc7

Schematic illustration of borophene with few layers exfoliated by the probe ultrasonic assisted solvothermal treatment process.

PMC9055579

d0ra03492d-f1.jpg

0.44416

78ed905115f7488eb4acf6dff624342c

Optical microscope photos, SEM images, and TEM images of borophene with few layers exfoliated in different solvents: acetone (a–c), DMF (d–f), acetonitrile (g–i), ethanol (j–l), and NMP (m–o) (the insets show the Tyndall effect of borophene dispersion with few layers).

PMC9055579

d0ra03492d-f2.jpg

0.384438

de2238997e80422988fd8e2ceca0d51d

AFM images and the corresponding height profiles of borophene with few layers exfoliated in different solvents: acetone (a and b), DMF (c and d), acetonitrile (e and f), ethanol (g and h), and NMP (i and j), respectively.

PMC9055579

d0ra03492d-f3.jpg

0.414126

b721a50effe94f6a91273cac3cef82c0

Statistical data showing the lateral flake size average (a) and the thickness (b) of borophene with few layers in acetone.

PMC9055579

d0ra03492d-f4.jpg

0.423229

0fb06dc1bd09489abb165a277f305291

SEM images with low resolution (a) and high resolution (b) and TEM images of borophene with few layers obtained by probe ultrasonic assisted solvothermal exfoliation in acetone and corresponding FFT pattern of the selected area (c–e).

PMC9055579

d0ra03492d-f5.jpg

0.44754

9ba1792e2b7d48c78d4aa57af11e0e24

HAADF STEM and corresponding EELS elemental mapping images of borophene with few layer boron sheets by probe ultrasonic assisted solvothermal exfoliation, followed by centrifugation treatment in acetone: STEM image (a), boron (b), carbon (c), and oxygen (d).

PMC9055579

d0ra03492d-f6.jpg

0.41099

e14d8db427de4ff2aa9c9790301ec3e8

XRD patterns (a) and Raman spectra (b) of bulk B and borophene with few layers obtained by probe ultrasonic assisted solvothermal exfoliation.

PMC9055579

d0ra03492d-f7.jpg

0.402509

5f0ca5ddd95e4ae7a30e34d6fc9883bb

XPS spectra (survey) (a) and short scan XPS B 1s (b) of bulk B and borophene with few layers obtained by probe ultrasonic assisted solvothermal exfoliation.

PMC9055579

d0ra03492d-f8.jpg

0.410164

5a8e3deaef83423fa7e32fa38091ec15

(a) The high-resolution Co 2p, (b) Cu 2p, (c) C 1s, and (d) N 1s XPS spectra of the CD@CuCoPBA nanocomposite. (e and f) Low- and high-magnification SEM and (g and h) TEM images of the CD@CuCoPBA nanocomposite.

PMC9055645

d0ra01439g-f1.jpg

0.423299

cebd5be0385945608e2dcae813444b97

Cell viability of CD@CuCoPBA with different (a) CD@CuCoPBA concentrations and (b) MTT incubation time intervals, (c) cell uptake behaviors of CD@CuCoPBA incubated with MCF-7 cell for 1 h. Confocal microscopy images were obtained through different channels according to the CD@CuCoPBA emission (the scale bar corresponds to 50 μm).

PMC9055645

d0ra01439g-f2.jpg

0.452372

1f63a5f2fd9c41d6b07211df2dd51c3b

EIS Nyquist plots of the determination procedure for EGFR by using (a) the CuCoPBA- and (b) CD@CuCoPBA-based sensors, including the bare AE, materials/AE, Apt/materials/AE, and EGFR/Apt/materials/AE; (c) EIS responses of the Apt/CD@CuCoPBA/AE against EGFR with different concentrations of 0.001, 0.005, 0.05, 0.5, 5, 50, 500 and 1000 pg mL−1; (d) dependence of the ΔRct values on the EGFR concentration detected by the Apt/CD@CuCoPBA/AE. The linear part of the calibration curve is shown in the inset of (d). (e) The sensing selectivity measurement. The ΔRct values of the proposed electrochemical sensor by separately adding different interferents, including HER2, IgG, IgE, PSA, BSA, and CEA with the concentration of 0.5 pg mL−1, EGFR of 5.0 fg mL−1, and their mixture. (f) Reproducibility, (g) stability, and (h) regeneration of the proposed aptasensors for detecting EGFR with the concentration of 5.0 fg mL−1 (n = 3).

PMC9055645

d0ra01439g-f3.jpg

0.416736

978146b2d0c6437385c5c7e724f0a04d

(a) EIS Nyquist plots and (b) CV curves for testing the whole detection procedure of MCF-7 cells using the proposed CD@CuCoPBA-based aptasensor. (c) EIS responses of the Apt/CD@CuCoPBA/AE against MCF-7 cells with different concentrations of 5 × 102, 1 × 103, 5 × 103, 1 × 104, 5 × 104, and 1 × 105 cell per mL; (d) dependence of the ΔRct values on the MCF-7 cell concentration using the Apt/CD@CuCoPBA/AE. The linear parts of the calibration curves are shown in the inset of (d). (e) The ΔRct values of CD@CuCoPBA-based aptasensor for detecting L929 and C6 cells with the concentration of 500 cell per mL. (f) Reproducibility of the CD@CuCoPBA-based sensor for detecting MCF-7 cells with the concentration of 100 cell per mL (n = 3).

PMC9055645

d0ra01439g-f4.jpg

0.406426

4c8b22e538a24e019003231bec16acf7

Schematic working principle of the developed functional DNA biosensors that use SPMs A, B and C loaded with aptamers or DNAzymes and enzyme-coated SiO2 microbeads for read-by-eye distance-measuring quantification of adenosine, interferon-γ, and Pb2+ (as three model analytes) based on starch-hydrolysis-adjusted wettability change in strip-like μPADs, respectively.

PMC9055663

d0ra04619a-f1.jpg

0.42062

148d380c7f3c4203ae951792a6563583

Flowing distance (FD) results of the colored reagent obtained from different solutions in the strip-like μPADs. The scale bar is 3.5 mm.

PMC9055663

d0ra04619a-f2.jpg

0.395553

70b4d68b059a4a0f9c62be2ab396c72f

(A) Flowing distance (FD) results obtained from a starch solution (0.5 wt%), a blank sample (PBS buffer without the analyte), 50 μM adenosine, and three other sorts of small molecules (100 μM each). (B) Calibration curve that describes the relationships between the FD change (ΔFD = FDadenosine − FDblank) and the logarithm values of the adenosine concentrations (log[adenosine]) in the buffer samples. The linear regression equation is y = 2.7651x + 1.8551 (R = 0.9914). Each error bar represents a standard deviation across three replicate experiments.

PMC9055663

d0ra04619a-f3.jpg

0.399264

e59ea8a40bf341fe855e14398317427a

(A) Flowing distance (FD) results obtained from a blank sample (HEPES buffer without the analyte), 30 nM interferon-γ, and three other sorts of proteins (10 μM each). (B) Calibration curve that describes the relationships between the FD change (ΔFD = FDinterferon-γ − FDblank) and the logarithm values of the interferon-γ concentrations (log[interferon-γ]) in the buffer samples. The linear regression equation is y = 2.7406x + 2.6643 (R = 0.9961). Each error bar represents a standard deviation across three replicate experiments.

PMC9055663

d0ra04619a-f4.jpg

0.411336

1cb7f95ea06d4dec9af1042e71fd0170

(A) Flowing distance (FD) results obtained from a blank sample (HEPES buffer without the analyte), 50 nM Pb2+, and twelve other sorts of metal ions (1 mM each). (B) Calibration curve that describes the relationships between the FD change (ΔFD = FDPb2+ − FDblank) and the logarithm values of the Pb2+ concentrations (log[Pb2+]) in the buffer samples. The linear regression equation is y = 2.7252x + 2.3799 (R = 0.9973). Each error bar represents a standard deviation across three replicate experiments.

PMC9055663

d0ra04619a-f5.jpg

0.431841

f140e3d9ff564148ba070acbf4931b47

(a) Ball and stick models of IL ions and peptide dimer. (b) Simulation boxes of peptide dimer in 10% and 70% (w/w) EAM, EAN, and TEAM ILs. Color scheme: cation-transparent surface, anion, water-stick, peptide-new cartoon.

PMC9056671

d0ra06609e-f1.jpg

0.434459

149699aff5b1490593450c6455ab72de

(a) Free energy profile of peptide (16–22) dimer in water and corresponding conformations of minima. (b) RDFs of water hydrogen and oxygen atoms around amide oxygen and hydrogen atoms of the peptide backbone. The dashed lines represent the number integral for the corresponding RDF.

PMC9056671

d0ra06609e-f2.jpg

0.436529

d9601f46ae3d4d26864a8ff5c7d572d3

Free energy profiles of peptide (16–22) dimer in 10% and 70% (w/w) EAM (a and b), EAN (c and d), and TEAM (e and f) ILs and corresponding conformations of minima.

PMC9056671

d0ra06609e-f3.jpg

0.441426

0f0901c59df8456badfe7cf8a385dad2

Semi-log representation of RDFs of cation, anion, and water around amide oxygen and hydrogen atoms of the peptide backbone for the corresponding minimum free energy conformations in 10% (w/w) EAM (a and b), EAN (c and d), and TEAM (e and f) IL. The dashed lines represent the number integral for the corresponding RDF.

PMC9056671

d0ra06609e-f4.jpg

0.481168

b1363b102add47aa9a848c33bfe81e55

Semi-log representation of RDFs of cation, anion, and water around amide oxygen and hydrogen atoms of the peptide backbone for the corresponding minimum free energy conformations in 70% (w/w) EAM (a and b), EAN (c and d), and TEAM (e and f) IL. The dashed lines represent the number integral for the corresponding RDF.

PMC9056671

d0ra06609e-f5.jpg

0.477216

9746d28b91ff42ec93b11ec4dc52899f

The average solvent-accessible surface area of the peptide dimer overall solutions with changing the average Ψ.

PMC9056671

d0ra06609e-f6.jpg

0.479917

b0db83271924414aab6544946b551252

Comparative surface plasmon resonance peaks of reaction mixtures for AgNPs synthesis in WPE, and extracts from TDZ-induced cell cultures, and MLN-induced cells cultures. Controls; cell extracts only, Ag, and WPE only.

PMC9057356

d0ra08419k-f1.jpg

0.469414

20f89becf2f9402695497250e757718d

Dose–response recorded in terms of AgNPs yield when supplemented with different concentrations of WPE and extracts from TDZ-induced cell cultures, and MLN-induced cell cultures at a constant concentration of AgNO3 (2 mM).

PMC9057356

d0ra08419k-f2.jpg

0.454465

cada31a3d0c74d42b49ef73c44001d9c

Time course analysis of yield of AgNPs biosynthesized through extracts of TDZ-induced cell cultures, MLN-induced cell cultures, and whole plant of F. indica.

PMC9057356

d0ra08419k-f3.jpg

0.408883

c0b48e76a6194a4d8c1d556ebc4f242c

Comparison of the reaction mixture of AgNPs synthesized through extracts of MLN induced cell cultures (left), TDZ-induced cell culture (center), and whole plant (right) after 48 hours of incubation.

PMC9057356

d0ra08419k-f4.jpg

0.528795

d28e47c1647c47e2b30714af731eed05

Transmission electron microscopy (TEM) based images of AgNPs synthesized via extracts from (a) MLN-supplemented cell cultures (b) TDZ-supplemented cell cultures and (c) naturally grown F. indica.

PMC9057356

d0ra08419k-f5.jpg

0.459738

bf7002e9d7174874801c4eacf3835d56

Size distribution of AgNPs synthesized through aqueous extracts of (a) TDZ-induced cell cultures (b) MLN-induced cell cultures and (c) naturally grown F. indica.

PMC9057356

d0ra08419k-f6.jpg

0.603377

0fa05fc72ef64569b32881b0e83498e1

Venn diagram for the comparative analysis of compounds detected through LC-MS/MS.

PMC9057356

d0ra08419k-f7.jpg

0.549445

b02e05bbbecf47fa937958994b9e65d0

Reactive oxygen quantification in bacterial cultures grown in the presence of different concentrations of AgNPs synthesized in MLN-based in vitro callus cultures.

PMC9057356

d0ra08419k-f8.jpg

0.441635

8706347f48a44fbf9fda1e8d0ed942a3

The topographic distribution of the pelvic resection zones.

PMC9057553

JDRS-2022-33-1-117-131-F1.jpg

0.414584

0424858b25904de089766fb363b0f704

Extended lateral incision with the flexible lateral decubitus position.

PMC9057553

JDRS-2022-33-1-117-131-F2.jpg

0.423293

9de788037d024365a9aa8f12c6943130

(a) Preoperative anteroposterior (AP) pelvis radiograph of the 42-year-old patient with recurrent malignant giant cell tumor (mGCT) involving almost the entire left hemipelvis. (b) 3-dimensional (3D) reconstruction on computed tomography (CT) with the soft tissue component of the recurrent tumor involving the left hemipelvis of the same patient. (c) 3D reconstruction on CT with only bony structures revealed the massive destruction, particularly in zone II and zone III. (d) Postoperative AP pelvis radiograph at 10 years. (e) 3D CT AP view of the patient who underwent combination of allograft and cemented arthroplasty after Zone I + II + III resection. (f) 3D CT lateral view of the patient who underwent combination of allograft and cemented arthroplasty after Zone I + II + III resection. (g) Excellent functional result was obtained with 80% musculoskeletal tumor society (MSTS) score.

PMC9057553

JDRS-2022-33-1-117-131-F3.jpg

0.419355

cdeaee192355473aba7995d1a51a610e

(a) Preoperative axial computed tomography (CT) image of our 45-year-old patient with right periacetabular osteogenic sarcoma (OGS). (b) Intramedullary OGS in the right periacetabular region in coronal T2 contrast-enhanced magnetic resonance images (MRI). (c) Postoperative anteroposterior (AP) pelvis radiograph at three years. (d) Aseptic loosening complication at the allograft and cement interface of hip arthroplasty in the 44th month follow-up of our patient who underwent type I + II resection. (e) Postoperative 10th year control AP pelvis radiography after revision with acetabular cage + cemented constraint cup. (f) Excellent functional result was obtained with 85% musculoskeletal tumor society (MSTS) score. He walks without any assistive device.

PMC9057553

JDRS-2022-33-1-117-131-F4.jpg

0.393847

1edd14613f69487cbb281e2b0e43a3dd

(a) Preoperative anteroposterior (AP) pelvis radiograph of the 35-year-old patient with high grade chondrosarcoma (CHS) involving right hemipelvis. (b) Intraosseous chondroid matrix mineralization and cortical destruction are seen in axial T2 contrast-enhanced magnetic resonance images (MRI) in right ileum. (c) Postoperative 6th year AP pelvis radiography. Excellent functional result was obtained with 80% musculoskeletal tumor society (MSTS) score. She walks without any assistive device. (d) It was possible to remove the tumor en bloc by performing wide resection with the surrounding gluteus maximus and iliacus muscle. (e) Allograft was placed anatomically between the pubic, ischium and residual crescent bone fragments, and the graft was supported vertically with lumbopelvic stabilization in addition to the combination of cemented constraint hip arthroplasty.

PMC9057553

JDRS-2022-33-1-117-131-F5.jpg

0.434124

7e161c4add1d4d359d810c0131177e4a

(a) Preoperative anteroposterior (AP) pelvis radiograph of the 43-year-old patient with giant high grade MMT causing destruction of the right hemipelvis (b) Coronal CT images showed how much the soft tissue component pushes the visceral organs to the contralateral side, and destruction of the tumor, especially in the periacetabular area and ischium. (c) Considering that the large tumor even crossed the midline medially, only marginal resection could be made, as can be understood from the specimen. (d) Osteosynthesis with reconstruction plates between the residual ileum and pubic bone and the perfectly cut allograft. Hip joint restoration with large-head proximal femur resection prosthesis for stability. (e) Postoperative 5th months AP pelvis radiography. This patient died of lung metastasis with deep infection before local recurrence was observed in the 6th postoperative month. Poor functional result with the MSTS score of 26% and could only be mobilized with 2 crutches.

PMC9057553

JDRS-2022-33-1-117-131-F6.jpg

0.40724

bab24d4f62634a8681a0fd0a57dc66f6

Patient flow chart. PH, pulmonary hypertension.

PMC9058072

fcvm-09-814557-g001.jpg

0.438899

bd391e8d9e3f4d2ea0b386f8805d2f1b

The relative feature importance of predictor variables included in the gradient boosting decision tree for predicting the death of pregnant women with pulmonary hypertension. NT-proBNP, N-terminal brain natriuretic peptide; PASP, pulmonary artery systolic pressure; ALB, albumin; RA, right atrium; APTT, activated partial thromboplastin time; RDW, red cell distribution width; PT, prothrombin time; WHO, World Health Organization.

PMC9058072

fcvm-09-814557-g002.jpg

0.411201

a58daaabbe3246cb811f786f102595da

The ROC curves of significant predictors. NT-proBNP, N-terminal brain natriuretic peptide; PASP, pulmonary artery systolic pressure; ALB, albumin.

PMC9058072

fcvm-09-814557-g003.jpg

0.541752

46bb342164e2476799861e9a7efdfc69

Schematic figure of the (a) Model A and (b) Model B versions of vertical diffusion cell.

PMC9058821

gr1.jpg

0.491779

90c96a6766bc4a9b8f6902a41d7191b1

Schematic illustration for comparison of (a) Franz cell apparatus and (b) the novel modified vertical diffusion cell apparatus.

PMC9058821

gr2.jpg

0.468305

f21a1d3b4d79418a93216eca5ab96edb

Three-dimensional views of various constructions of modified vertical diffusion cell ((a) Model A, (c) Model B) and technical drawings of these devices ((b) Model A, (d) Model B).

PMC9058821

gr3.jpg

0.460014

ef17522746bb49cab68b77f472b9568d

Photo about ‘Model A’ version of the modified vertical diffusion cell. The inset shows in more detail what is the right combination of the donor (A1) and the acceptor (D8) compartments. The figure also shows the essential parts of cell: donor chamber (A), membrane (B1), sample (B2), silicone insert (B3), glass plate or cover plate (B4) stainless steel ball joint clip (C), acceptor chamber (D1), sampling inlet (D2), second overflow inlet (D3), second inlet tap (D4), first inlet (D5), first inlet tap (D6), ground inlet (D7), spherical edge of the acceptor chamber (D8), magnetic stirrer bar (E), Hamilton syringe (F), drip funnel (buffer tank) (G1), glass tap (G2).

PMC9058821

gr4.jpg

0.428323

2ea573814e234956b2ef68bf1fc40ca8

Schematic figure of operational system which contains the necessary main background instruments: double-walled vessel (H), sealed cap (I), temperature control thermocouple (O1), heated magnetic stirrer (O2), primary tempering fluid in baker, peristaltic pump (O3).

PMC9058821

gr5.jpg

0.464202

9dc4c1791cb749ca95512fbbae9101d8

Bland-Altman plot for inter-rater reliability analysis of each acetabulum location. The limits of agreement are shown as dotted, black lines with 95% confidence intervals (green and red areas), bias (as dotted black line) with 95% confidence intervals (blue area), and regression fit of the differences of the means (as solid black line). (a) Medial wall. (b) Posterior wall. (c) Superior wall. (d) Anterior wall.

PMC9059077

fx1.jpg

0.417251

d73eb2dad8804428ae9a49b2f9fda852

Postoperative coronal computed tomography image of the hip joint. The axial image was sliced at the center of the head.

PMC9059077

gr1.jpg

0.416695

1cf903fe1e9e434f811dc0363ee4e4c5

Preoperative axial computed tomography image of the hip joint. Image is at the same level as the postoperative image and sliced at the center of the head. Anterior, posterior, and medial walls were measured with the maximum elliptic mean value and did not include the cortex. The measurement of the medial wall was taken at the bisector of the anterior-posterior diameter of the acetabulum without a double floor.

PMC9059077

gr2.jpg

0.428385

e3e531ed23174c5a9c3eda0ec7a21b26

Maximum elliptic mean. In the superior wall, the coronal image at the center of the medial wall was used to measure the maximum elliptic mean without the cortical bone.

PMC9059077

gr3.jpg

0.458431

f649cca14cb34207bd431dc488fb19b3

Scatter plot of reliability at each region of the acetabulum. The plot shows inter-rater reliability assessed at the (a) superior, (b) anterior, (c) posterior, and (d) medial walls.

PMC9059077

gr4.jpg

0.431432

3f866fd5f6994048817bff0384b40828

Tripodal molecules 1a–4b.

PMC9059549

c8ra06647g-f1.jpg

0.476602

69fd53d9ff65462ca66fe57d490de548

Partial 1H NMR spectra of L1, 1a and 1b in d6-DMSO solvent.

PMC9059549

c8ra06647g-f2.jpg

0.416177

a4e780319ba14eac87c73ef6abda5954

The relative 1H-NMR chemical shift (in ppm) of H4–H7 protons (Mol = 1a–4b and L = L1–L4).

PMC9059549

c8ra06647g-f3.jpg

0.479801

8e8de04356c84879a0129e9ef2967212

Molecular structures of 1a, 1b, 2a and 2b evaluated at MPW1PW91/6-311+G(d,p) level of theory and basis function showing the cyclic benzene trimer (CBT) between three benzene rings of benzimidazolyl units.

PMC9059549

c8ra06647g-f4.jpg

0.422471

f72f68a76f5a40bb935925f50e1e3d83

MESP topography distribution of 1a, 1b, 2a and 2b evaluated at MPW1PW91/6-311+G(d,p) level of theory and basis set. The consecutive contours are separated by 0.02 a.u.

PMC9059549

c8ra06647g-f5.jpg

0.399157

9939cfcde7f24c08a92cd53c47dd3c27

The substitutions of nonstructural proteins during the five waves of SARS-CoV2 pandemic in Iran.

PMC9060343

pone.0267847.g001.jpg

0.429616

9f9de0d38ceb4c2cab084317e0c80f1f

Phylogenetic tree of SARS-CoV2 full-length genomes constructed by MEGA 7.The Neighbor joining method was used with 1,000 bootstrap replicates. The tree contains 54 SARS-CoV2 sequences of these study compared to the reference sequence from GISAID and some other sequences from each clade. In this tree the reference sequence is marked by white circle and sequences of this study were marked as follow: The 1st wave black circle, the 2nd wave inverted black triangle, the 3rd wave with black square, the 4th wave with black triangle and the 5th wave with black diamond.

PMC9060343

pone.0267847.g002.jpg

0.433668

afd72e90c6354a07a199feb533a1cf3c

Synthesis of a series of 1,2-bis(N-alkylimidazolium)ethane BF4− salts.

PMC9060431

c8ra09208g-f1.jpg

0.445908

0beab14082b54dea81d3a5fac4ba840b

500 MHz 1H NMR spectrum of C8 in acetone-d6.

PMC9060431

c8ra09208g-f2.jpg

0.539057

0ca8311c85c74edead749abd98d1e431

Relationship between the side-chain length and melting temperature/first solid–solid phase transition temperature for the synthesized dicationic imidazolium BF4− salts.

PMC9060431

c8ra09208g-f3.jpg

0.480821

b66546a5bd624663a80860c266505415

DSC thermograms of C6 (blue) and C7 (red) (2nd heating scans, heating rate = 10 K min−1, N2).

PMC9060431

c8ra09208g-f4.jpg

0.449368

46a8f762fc024c4abb4e4b60e9aa1570

DSC thermograms of C11 (blue) and C12 (red) (2nd heating scans, heating rate = 10 K min−1, N2). The small Tm peaks of C11 and C12 correspond to the small ΔSf values: 18 and 17 J K−1 mol−1, respectively.

PMC9060431

c8ra09208g-f5.jpg

0.390002

a355f0cad15d46f69613daf5239cff6c

POM images of (a–d) C6 and (e–h) C7 during cooling from their isotropic phases: (a) plastic crystal phase at 100 °C, (b) dark domains aligned at 80 °C before the solid–solid phase transition, (c) plastic crystal phase cooled to room temperature, and (d) crystalline texture after slow crystallization at room temperature. For C7: (e) plastic crystal phase at 150 °C, (f) dark domains aligned at 100 °C, (g) undulated pattern in the lower phase at 50 °C, and (h) cracked crystalline texture at room temperature. All scale bars correspond to 200 μm.

PMC9060431

c8ra09208g-f6.jpg

0.423126

de07b3807614413586573521d526997f

POM images of C12 during cooling from its isotropic phase: (a) at 200 °C, (b) at 150 °C, (c) at room temperature. All scale bars correspond to 200 μm.

PMC9060431

c8ra09208g-f7.jpg

0.433119

ad5236417fd043cbb6c46ba423a53aaa

1D wide-angle X-ray scattering spectra of (a) the plastic crystal C7 and (b) the liquid crystal C12 at different temperatures.

PMC9060431

c8ra09208g-f8.jpg

0.45312

8fae54621ee24f3ab9a36c164a251c43

Ionic conductivity (left axis) as a function of temperature while cooling combined with DSC cooling traces (right axis) for (a) C6 and (b) C11.

PMC9060431

c8ra09208g-f9.jpg

0.527817

300ab1a0dc664fef90b2cc1ffb663b3d

Comparison of patient satisfaction between the two groups.

PMC9060996

CMMM2022-4820090.001.jpg

0.494647

fdca8e2cb63e48cfbed5ea6667340b87

The concentric cell (BLM) for transport experiments (a relatively thick layer of immiscible fluid is used to separate the source and receiving phases).

PMC9062073

c8ra09118h-f1.jpg

0.5735

b4833f0594834716b8d6f58d861ea8be

Displacement ellipsoid plot (50% probability) and atom-numbering scheme for PTC. The H atoms are drawn as small spheres of arbitrary radii. Selected bond lengths (Å) are P1–O1 = 1.4718(16), P1–N1 = 1.7306(19), P1–N2 = 1.616(2) and P1–N3 = 1.615(2), and selected bond angles (°) are O1–P1–N1 = 103.29(9), O1–P1–N2 = 116.39(9), O1–P1–N3 = 116.53(10), N1–P1–N2 = 109.35(10), N1–P1–N3 = 107.28(10) and N2–P1–N3 = 103.66(10).

PMC9062073

c8ra09118h-f2.jpg

0.438626

54e07f502b984f6184dc0bcb37881f08

A view of one-dimensional array of molecules, built from NH⋯O hydrogen bonds. The weakly occupied atoms and hydrogen atoms not involved in hydrogen bonding were omitted for the sake of clarity.

PMC9062073

c8ra09118h-f3.jpg

0.496434

5eca9c6aca344ac6af52cd6b7aee0976

The possible tautomeric equilibrium in solution.

PMC9062073

c8ra09118h-f4.jpg

0.441593

b92bc9e424f34691a36c5ec2bd3e3911

Transport percentages for seven metal cations by PTC carrier in different organic solvents.

PMC9062073

c8ra09118h-f5.jpg

0.397876

536bbc4935e54c5092bd34cc06472a27

Molar conductance–mole ratio plots for the PTC–Cu(ii) complex in NB at different temperatures.

PMC9062073

c8ra09118h-f6.jpg

0.453798

539246d95c9d4e8d9864826dac44f6b0

Profiling gene expression and genomic accessibility in the plastic and fate-restricted RPE. The developing (dev.) eye at E4 (A) and posterior eye at E5 (B) are shown with RPE and neuroepithelium (NE; presumptive neural retina) labeled. A regenerating NE is observed 3 days PR if treated with FGF2 at E4 (C), but no regeneration is observed 3 days PR and FGF2 treatment at E5 (D). At 3 days PR, no regeneration is observed if surgery is performed at E4 (E) or E5 (F) in the absence of FGF2. Scale bars in (A–F) are each 200 μm. Asterisk (*) denotes the FGF2 bead. (G) Schematic summarizes the collection of RNA-seq and ATAC-seq samples used in this study. (H) Principal component analysis summarizes the variation present in RNA-seq normalized gene expression values across 18 samples and six tested conditions. (I) Heatmap displays blind hierarchical clustering of normalized ATAC-seq peak signal for each sample.

PMC9062105

fcell-10-875155-g001.jpg

0.460296

4063b66cd12a4283b9a34c82f4a5cfd3

Clustering of differentially expressed genes reveals expression programs associated with RPE commitment and reprogramming. (A) Affinity propagation clustering was performed on all genes that are differentially expressed across the E4 to E5 window by the criteria |LFC| ≥ 1 and adj. p-value ≤ 0.05 (n = 2551), resulting in six clusters. Key genes are summarized on the right of heatmap. The heatmap intensity displays row-normalized gene counts. (B) Bubble charts display select gene ontology terms associated with each cluster. Adj. p-value is plotted on y-axis and the bubble size is proportional to number of terms identified in the analysis.

PMC9062105

fcell-10-875155-g002.jpg

0.497795

1972eeb4e5d245c59efa71a03dacbc91

Neural retina identity genes are active 6hPR + FGF2 at E4 and E5. Genome browser views display the VSX2 (A) and SIX6 (B) loci, with the normalized counts for ATAC-seq and RNA-seq assays summarized in the coverage tracks above. The highlighted pink area represents changes in promoter-region accessibility. Normalized gene expression counts are displayed for neural retina-associated genes PAX6 (C) LHX2 (D) and ASCL1 (E). The y-axes are log-transformed. Asterisk (*) denotes an Adj. p-value ≤ 0.05. n.s., not significant. (F) Two representative RPE explants are shown after 48 h in culture in the presence of FGF2 at E4 (left) or E5 (right). Red arrows point to RPE-derived neural retina from E4 explants. (G) Gene expression was measured via RT-qPCR using explants cultured in the presence of FGF2 at E4 or E5 and collected at the specified time points. Bar chart displays the average expression, and the error bars represent standard error of the treatment mean based on ANOVA. Each dataset is normalized to basal levels observed at time 0; y-axes are log2-transformed. qPCR significance: n.s. denotes not significant, * denotes p-value < 0.05, ** denotes p-value < 0.01, *** denotes p-value < 0.001.

PMC9062105

fcell-10-875155-g003.jpg

0.490809

88c6dfe2d7974381ba8692be9f8bf51f

Proliferation is tightly regulated across the window of RPE cell fate restriction. (A) EdU and DAPI fluorescence staining of embryo sections collected 24 h PR (24hPR) and FGF2 treatment was performed at either E4 (A) or E5 (B). Fluorescence channels are overlayed with differential interference contrast (DIC) image. 50 μm scale bar in (A) applies to both panels. Chart displays the proportion of EdU positive RPE cells observed 24hPR + FGF2 at E4 and E5 (C). Diamond represents mean proportion; asterisk (*) denotes Adj. p value <0.05. (D) Gene set enrichment analysis (GSEA) was performed on normalized RNA-seq expression values from 6hPR + FGF2 E4 and E5 samples. A low normalized enrichment score (NES = −3.4349458) for E2F targets indicates high enrichment in the E4 6hPR + FGF2 RPE and depletion from the E5 6hPR + FGF2 RPE. Each black vertical bar represents a gene in the pathway. (E) The row-normalized heatmap displays RNA-seq expression values for E2F targets genes and proliferation-associated factors. (F) RT-qPCR was used to measure E2F1 expression in E4 and E5 explants cultured in the presence of FGF2 and collected at the specified time points. Bar chart displays the average expression value, and the error bars represent standard error of the treatment mean based on ANOVA. Each dataset is normalized to basal levels observed at time 0; y-axes are log2-transformed. qPCR significance: n.s. denotes not significant, * denotes p-value < 0.05, ** denotes p-value < 0.01, *** denotes p-value < 0.001.

PMC9062105

fcell-10-875155-g004.jpg

0.421058

00af08b26d5f446394ab790099b27fad

Gene signatures associated with RPE maturation are broadly elevated in the E5 RPE. Normalized RNA-seq expression values are displayed for genes encoding RPE-associated transcription factors (A), the ion channel BEST1 (B), retinol metabolism and transport factors (C), melanin synthesis machinery (D), and melanosome-associated proteins (E). For (A–E), asterisk (*) denotes an Adj. p-value ≤ 0.05 and n.s., not significant. Relative gene expression was measured in RPE explants using RT-qPCR, and is displayed for OTX2 (F), MITF (G), RPE65 (H), and TYR (I). Bar chart displays the average expression, and the error bars represent standard error of the treatment mean based on ANOVA. Each dataset is normalized to basal levels observed at time 0; y-axes are log2-transformed. qPCR significance: n.s. denotes not significant, * denotes p-value < 0.05, ** denotes p-value < 0.01, *** denotes p-value < 0.001.

PMC9062105

fcell-10-875155-g005.jpg

0.433335

2838d38512364273ad5b292bb2ec0a42

RPE maturation is accompanied by differential accessibility at retinal development genes and homeobox transcription factor binding sites. (A) Venn diagram summarizes the overlap of up-regulated DARs (DARs with increased E5 accessibility) between the developing and 6hPR + FGF2 conditions, defined by a cut-off of Adj. p-value ≤ 0.05. (B) Similarly, Venn diagram displays down-regulated DARs (E4-enriched DARs) for the two conditions. DARs unique to either condition were annotated to the nearest gene TSS within 10 kilobases, and pathway enrichment analysis was performed for DARs enriched in the developing E5 RPE (C), the E5 6hPR + FGF2 RPE (D), the developing E4 RPE (E), or the E4 6hPR + FGF2 RPE (F). (G) The log fold change (LFC) of differentially accessible peak summits between the E4 and E5 conditions are plotted. Dashed lines represent a LFC cut-off of 1 that was used to select summits for motif analysis. HOMER motif detection software was used to identify transcription factor DNA motifs overrepresented in the summits of peaks differentially accessible across the developing (H) or 6hPR + FGF2 (I) RPE, and the top 15 enriched motifs are shown.

PMC9062105

fcell-10-875155-g006.jpg

0.485006

f82ce83f9e6642e98e2ab296416d89a4

Overrepresented motifs in open regions of the E4/E5 RPE. The monaLisa motif analysis software was used to bin differentially accessible summits by accessibility across the E4 and E5 conditions. Each bin was analyzed for the overrepresentation of transcription factor motifs found in the JASPAR 2022 database. Enriched motifs identified at −log(p-Adj.) >3 in any bin were recorded and select factors associated with the developing RPE (A) or 6hPR + FGF2 (B) samples were plotted. (C) Genome browser displays ATAC-signal proximal to DARs containing the OTX2 binding motif. The region of interest is highlighted in pink and associated motifs are summarized below the track.

PMC9062105

fcell-10-875155-g007.jpg

0.383984

5da69b21807e42b88f0eb4319a14c9d8

Final thematic map.

PMC9062181

fpsyg-13-859240-g001.jpg

0.42157

ddc7295af2b746b9bf41325f5a8a687e

Phylogenetic tree showing the relationship among nitrogen fixing, phosphorus and potassium solubilizing bacterial isolates, 16S rRNA gene sequences with reference sequences obtained through BLAST analysis. The trees were constructed using neighbor joining (NJ) with algorithm using MEGA 4 software (Tamura et al., 2007).

PMC9062246

gr1.jpg

0.417003

bc8f86e5b40c47b6a032e70a15161944

Study design, patient selection and evaluation. ICU, intensive care unit.

PMC9062462

bmjopen-2021-051971f01.jpg

0.361355

1f4196bee0b2413e9a4ee869b04eadbb

Results of global coagulation tests (gb and hSonoclot) in the low flow (N=11), high flow (n=34) and invasive ventilation (n=29) oxygen requiring subgroups. Presence of heparin-like effect (HLE) as defined as difference in Sonoclot trace at days 1, 3 in patients. The prevalence of HLE and changes in Sonoclot test was assessed as per oxygen requirement and survival (A–F).

PMC9062462

bmjopen-2021-051971f02.jpg

0.376933

3dbb78a4feaf4c92a91a6e774b99b2b0

Kaplan-Meir survival curves for patients (A—oxygen groups; B—presence of heparin-like effect).

PMC9062462

bmjopen-2021-051971f03.jpg