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0.526572
ff289623d33c4fd7808d9d487dd44d41
The Stress-strain curves for Ti-xNb-10Zr (x: 10 and 20; at. %) (a) alloys with low porosities, (b) alloys with high porosities.
PMC10302561
materials-16-04240-g008.jpg
0.454484
89848b98b7bf40488d7034e390b4411d
Results of electrochemical corrosion tests for Ti-xNb-10Zr (x: 10 and 20; at. %). (a) Alloys with low porosities; (b) Alloys with high porosities.
PMC10302561
materials-16-04240-g009.jpg
0.413915
4bd2c1824c3245e49ac31bdc13125393
(a) L929 and (b) Saos-2 cell viability results for all Ti-(x)Nb-10Zr (x: 10 and 20; at. %) alloys with low porosity (wlp), high porosity (whp), and the reference TiGR4 extracts for 1-day, 3-day, and 7-day measured by MTT assay. Data represent mean ± SD, n = 3. *, ** for p < 0.05.
PMC10302561
materials-16-04240-g010.jpg
0.378768
6b6f325fa39e4c70a547c0b436b63a9a
The images of (a) L929 and (b) Saos-2 cells upon exposure to Ti-xNb-10Zr (10, and 20; at. %) alloys with low porosity (wlp), with high porosity (whp), and the reference TiGR4 for 1-day, 3-day, and 7-day were taken by fluorescence microscope with 10× magnification.
PMC10302561
materials-16-04240-g011.jpg
0.450968
a0838839304b4f5b994851f4cecf987e
SEM images of viable (a) L929 and (b) Saos-2 cell lines for Ti-xNb-10Zr (x: 10 and 20; at. %) alloys with low porosity (wlp), with high porosity (whp), and the reference TiGR4 extracts for 1-day and 7-day. SEM images were taken with 500× magnification; 2500× magnification images taken from the red squares area in the 500× images.
PMC10302561
materials-16-04240-g012.jpg
0.443992
da4bdc5bd00b4ebe8e141a7b67b3a115
Adsorption of Fibronectin on Ti-xNb-10Zr (x: 10 and 20; at. %) with low porosity (wlp), with high porosity (whp), and the reference TiGR4 determined after 2 h incubation at 37 °C in a 5% CO2 atmosphere by ELISA method. Data represent mean ± SD, n = 3, * for p < 0.05.
PMC10302561
materials-16-04240-g013.jpg
0.478316
bb1f41eeeab6424990c2597432a8e57e
In vitro antifungal activity of the endophytic bacterium P. poae strain CO against F. graminearum strain PH-1 (A). The inhibition of mycelium growth in potato dextrose agar (PDA) and potato dextrose broth (PDB) mediums (B). TEM and SEM images of Fg strain PH-1 treated and untreated with endophytic bacterium strain CO (C). Data are mean value ± standard error of three replicates, and bars with the same letters are not significantly different in the LSD test (p < 0.05).
PMC10302817
plants-12-02277-g001.jpg
0.448494
a072d8e473d442439a2083851d7f2976
In vitro antifungal activity of CFSs at different concentrations against F. graminearum strain PH-1. The inhibition of mycelium growth (A,B) and the inhibition of colony number (C,D). Data are mean value ± standard error of three replicates, and bars with the different letters are significantly different in the LSD test (p < 0.05).
PMC10302817
plants-12-02277-g002.jpg
0.405743
d0aecc8f36dc4f94a8d59cf107db2997
In vitro antifungal activity of CFSs at different concentrations against spore germination and germ tube length of F. graminearum strain PH-1. Inhibition of spore germination (A), inhibition of germ tube length (B), and light microscope images of spores treated with different concentrations of CFSs (C). Data are mean value ± standard error of three replicates, and bars with the different letters are significantly different in the LSD test (p < 0.05).
PMC10302817
plants-12-02277-g003.jpg
0.488231
991f26c1eeae4f07a4e9ce92adaeea18
Effect of the CFSs at different concentrations on deoxynivalenol (DON) production. Data are mean value ± standard error of three replicates, and bars with the different letters are significantly different in the LSD test (p < 0.05).
PMC10302817
plants-12-02277-g004.jpg
0.377699
cc1a8d4c7f484854bf1ecc55ca9ac575
Phylogenetic tree of the endophytic bacterium P. poae strain CO isolated from garlic leaves, constructed using 16S rRNA gene sequences.
PMC10302817
plants-12-02277-g005.jpg
0.380112
8da51307f73f465bbf92ac31a81decdc
Inhibition of FSB under greenhouse condition using endophytic bacterium. The P. poae strain CO enhanced seedling-emergence growth in infected soil experimentally.
PMC10302817
plants-12-02277-g006.jpg
0.455776
1013dca5c0054ef69861c2064b508dcc
Protection of seedlings by the endophytic bacterium P. poae strain CO against FSB. The lesion size 7 days after inoculation was reduced in the coleoptiles of seedlings treated with Fg + P. poae compared to coleoptiles treated with Fg (A,B). Disease severity was reduced in the leaves of seedlings treated with Fg + P. poae compared to leaves treated with Fg (C,D). Data are mean value ± standard error of three replicates, and bars with the different letters are significantly different in the LSD test (p < 0.05).
PMC10302817
plants-12-02277-g007.jpg
0.424448
4a184f68862148e3af928f508bd28bf6
Detection of enzymatic activity and siderophore production of endophytic bacterium P. poae stain CO; zone diameter (mm) of cellulase, protease, amylase, and lipase of strain CO (A); colonies of P. poae surrounded by zones of extracellular enzymatic activity in the Petri dishes (B); siderophores unit (C); siderophores diameter (mm) (D); and the endophytic bacterium strain CO changed the CAS color from blue to purple, indicating its ability to produce siderophore (E). Data are mean value ± standard error of three replicates, and bars with the same letters are not significantly different in the LSD test (p < 0.05).
PMC10302817
plants-12-02277-g008.jpg
0.478662
cfe4690360ae41a3b4d8f802fe2c94c9
Detection of metabolites produced by endophytic bacterium P.poae strain CO using MALDI—TOF MS.
PMC10302817
plants-12-02277-g009.jpg
0.407636
bd6c3a4592a84be7850606efa5e0670b
Effect of endophytic bacterium P. poae strain CO on the growth of wheat seedlings (A): root length; (B): shoot length; (C): root fresh weight; (D): shoot fresh weight. (E): root dry weight; (F): shoot dry weight; (G): seedlings treated and untreated with CO. (H): Plant growth properties assay: indole-3-acetic acid (IAA); nitrogen fixation (NF); and phosphate solubilization (PS). Data are mean value ± standard error of three replicates, and bars with the different letters are significantly different in LSD test (p < 0.05).
PMC10302817
plants-12-02277-g010.jpg
0.454233
3e1445cf2c3249a394b10ad07307ba5a
Factor loading scores of dietary patterns identified using principal component analysis. DP 1—‘Unhealthy’ dietary pattern; DP 2—‘Fish-eggs-fruits-vegetables’ dietary pattern; DP 3—‘Cereals-confectionaries’ dietary pattern; DP 4—‘Legumes-dairy’ dietary pattern; DP 5—‘Meat-sugar-sweetened beverages’ dietary pattern. Red circle : food group perceived to be healthy; blue circle : food group perceived to be unhealthy; purple circle : food group consisting of healthy and unhealthy food items.
PMC10302866
nutrients-15-02819-g001.jpg
0.455704
f77f67bc24c14f6cb8459d456c88e48f
Top food sources of ‘Cereals-confectionaries’ dietary pattern. The food item’s percent distribution from the dietary pattern is based on the total amount consumed (g/day).
PMC10302866
nutrients-15-02819-g002.jpg
0.406156
26ce4d31b12a4b25a63c18e380acc93d
Performance of the OCT-guided strategy for retreatment decisions in comparison to the gold standard (visual acuity + OCT). DEX-i: dexamethasone implant; DME: diabetic macular edema; FAc-i: fluocinolone acetonide implant; OCT: optical coherence tomography; PRN: pro re nata; TAE: treat and extend; VA: visual acuity; anti-VEGF: anti-vascular endothelial growth factor.
PMC10303486
pharmaceutics-15-01607-g001.jpg
0.464583
a23a3e9eaea64e419d742b21b32c55cd
Linear regression plot for visual acuity (VA, ETDRS Scale) and central retinal thickness (CRT, panel (A)) or maximal retinal thickness (MRT, panel (B)) at the study visit. Correlation analysis performed using Spearman’s test showed a significant negative association for CRT (r = −0.39; p = 0.004) and MRT (r = −0.51, p < 0.0001).
PMC10303486
pharmaceutics-15-01607-g002.jpg
0.429531
9e59330c3e42401cb258ead39aace632
Schematic presentation of G-quadruplex-based label-free fluorescence aptasensor assay.
PMC10304070
molecules-28-04841-g001.jpg
0.550787
0a35deccf4714334956f98d12b8c4946
Fluorescence intensity of the aptamer/ThT mixture without OTA and with OTA (150 nM).
PMC10304070
molecules-28-04841-g002.jpg
0.425327
14673136458248bbb1d3f43be3c13a91
Molecular docking results between OTA and its aptamer.
PMC10304070
molecules-28-04841-g003.jpg
0.425942
9352637e41864ed79cc66a7df6d9ba55
Effects of different OTA aptamer (a) and ThT (b) concentrations on fluorescence intensity.
PMC10304070
molecules-28-04841-g004.jpg
0.574594
93172f83f675450c8ff193b941e38ac0
Calibration plot of changes in fluorescence intensity with various concentration of OTA.
PMC10304070
molecules-28-04841-g005.jpg
0.468139
fcc7af42f5944229b955f8d6ec343b85
Selectivity assessment of the aptasensor for OTA.
PMC10304070
molecules-28-04841-g006.jpg
0.425771
98c50f44db7e46208b296b691e950f5c
Characteristic information of temperature rise region of abnormally heating composite insulators.
PMC10304211
polymers-15-02715-g001.jpg
0.417819
7d1f70c8b5364d2cacd8a2450d71fd19
Abnormal heating composite insulator temperature rise area range and quantity statistical information. (a) Number of temperature rise areas; (b) range of temperature rise areas.
PMC10304211
polymers-15-02715-g002.jpg
0.435181
9978e892a30e4003843f87e61fd05ff0
Experimental measurement of temperature rise data in high- and low-humidity environments. (a) Low-humidity condition (30%RH); (b) high-humidity conditions (75%RH).
PMC10304211
polymers-15-02715-g003.jpg
0.447918
b8de139ec57a43c1a82e55f91f59f6ef
Diagram of the test arrangement.
PMC10304211
polymers-15-02715-g004.jpg
0.427475
03b9368067504dca85c547a07fa086c4
Infrared images of composite insulators with different defect conditions. (a) Sheath ageing (S1); (b) decay-like (S2).
PMC10304211
polymers-15-02715-g005.jpg
0.462679
c53a4dd9974b4de69024f51293d99b89
Microscopic appearance of the surface of the mandrel in the heating area of two specimens. (a) S1 test sample; (b) S2 test sample.
PMC10304211
polymers-15-02715-g006.jpg
0.542139
22a60fc5093b4797af9a49fea8b29d64
TGA results of different samples.
PMC10304211
polymers-15-02715-g007.jpg
0.424039
4872038c7a2f446585fd04f02ff1ada5
Composite insulator model.
PMC10304211
polymers-15-02715-g008.jpg
0.437938
8faac247903546c28e2ea85509c9b620
Dielectric equivalent circuit diagram.
PMC10304211
polymers-15-02715-g009.jpg
0.436335
df1653b3517c4132a5170fa728d29c06
Schematic diagram of the heat transfer process of composite insulator. (a) Mandrel deterioration; (b) sheathing problems.
PMC10304211
polymers-15-02715-g010.jpg
0.443432
8844b767035e4a7faa20bb3ea514583b
Surface temperature rise distribution of composite insulator. (a) Mandrel deterioration; (b) sheath ageing.
PMC10304211
polymers-15-02715-g011.jpg
0.414536
350f025b899c4c0e8969a63bba593549
Temperature rise distribution curve of high-voltage end of composite insulator. (a) Mandrel deterioration; (b) sheath ageing.
PMC10304211
polymers-15-02715-g012.jpg
0.44221
2dd11109d3324a6b9dbc7d98ba388f4a
Temperature rise gradient curve of high-voltage end of composite insulator. (a) Mandrel deterioration; (b) sheath ageing.
PMC10304211
polymers-15-02715-g013.jpg
0.423031
c848c817bc9747a7bd7bf96dee534ebf
Current density distribution at high-voltage end of composite insulator. (a) Mandrel deterioration; (b) sheath ageing.
PMC10304211
polymers-15-02715-g014.jpg
0.425
155c5fcd31f443eba376c5865c624ed3
Radial conduction heat flux density distribution at high-voltage end of composite insulator.
PMC10304211
polymers-15-02715-g015.jpg
0.425354
aa982642ad12412fb895c5a3d6542501
Axial temperature gradient distribution of high-voltage end of composite insulator. (a) Mandrel deterioration; (b) Sheath ageing; (c) axial temperature gradient distribution at the high-voltage end.
PMC10304211
polymers-15-02715-g016a.jpg
0.400792
51cc4abb205443cea74c9eb5e659654a
Statistics of characteristic coefficient of temperature rise gradient.
PMC10304211
polymers-15-02715-g017.jpg
0.428657
5ac4ce19849e4cf88c7ca14edd9ed548
Temperature rise gradient curves of S1 and S2. (a) Axis temperature rise gradient curve of S1; (b) axis temperature rise gradient curve of S2.
PMC10304211
polymers-15-02715-g018.jpg
0.530226
041279a4793c47b3a50f345ac35abbf9
Chemical structures of U50,488, salvinorin A, and 16-bromo salvinorin A. The green dashed box indicates the C2 position of previously investigated analogues of salvinorin A [65].
PMC10304272
molecules-28-04848-g001.jpg
0.442336
3fbc619ef9fe4b968f6c7903854a4dec
Results of the warm water tail withdrawal assay in vehicle-, salvinorin A (SalA; 1.0 mg/kg)-, or 16-bromo SalA (16-BrSalA; 1.0 mg/kg)-treated mice (n = 8–11/treatment). (a) Effect of treatment on % maximal possible antinociceptive effect as a function of time. (b) Area under the curve (AUC) data for each treatment. * p < 0.05, ** p < 0.01, and *** p < 0.001 compared to the vehicle (Dunnett’s test).
PMC10304272
molecules-28-04848-g002.jpg
0.396302
8f7968e808874db2a3d3f6fc7f63fdc0
Anticocaine effects of 16-BrSalA. (a) Total active lever responding during cocaine (20 mg/kg) + cue-induced reinstatement following vehicle, SalA (0.3 mg/kg), or 16-BrSalA (0.3, 1.0 mg/kg) treatment (n = 6; data partially reproduced from [60]). (b) Time course of cocaine (20 mg/kg) + cue reinstatement data (with an inset showing summed totals) displaying the effect of nor-binaltorphimine (nor-BNI; 10 mg/kg) on 16-BrSalA (1 mg/kg) treatment (n = 3). (c) Cocaine-induced (20 mg/kg) ambulatory counts (with an inset showing summed totals) in vehicle- and 16-BrSalA (1.0 mg/kg)-treated rats (n = 6/treatment). (d) Total number of cocaine (1 mg/kg) infusions (and break point/number of responses) earned on a progressive ratio schedule of reinforcement following vehicle, U50,488 (10.0 mg/kg), U69,593 (1.0 mg/kg), SalA (0.3, 1.0 mg/kg), or 16-BrSalA (1.0, 2.0 mg/kg) treatment (n = 14). * p < 0.05, ** p < 0.01, and *** p < 0.001 compared to the vehicle (Dunnett [a,d] and Tukey [b,c] tests). # p < 0.05, ## p < 0.01, and ### p < 0.001 compared to + nor-BNI (Tukey test).
PMC10304272
molecules-28-04848-g003.jpg
0.449518
c0fb930d9481488491c53c3ce852a5e3
Effect of 16-BrSalA on dopamine (DA) transporter (DAT) function. DA uptake kinetics over 0–4 μM concentrations in vehicle- and 16-BrSalA (500 nM)-treated nucleus accumbens (NAcc) (a) as well as dorsal striatal (dSTR) (b) tissue suspensions as measured by rotating disk electrode voltammetry (RDEV) using a low to infinite trans model (n = 9 samples/region/treatment). (c) 4-[4-(dimethylamino)styryl]-N-methylpyridinium (ASP+) uptake kinetics over 0–16 μM concentrations in vehicle- and 16-BrSalA (10 μM)-treated HEK-293 cells coexpressing the DAT and kappa opioid receptor (KOR) as measured via time-resolved fluorescence imaging (auto-fluorescence units/s (AFU/s), n = 17–51 cells/concentration/treatment). Effect of nor-BNI (1 μM) treatment on 16-BrSalA (500 nM)-induced change in uptake of 2 μM DA in rat NAcc (d) and dSTR (e) tissue suspensions as measured through RDEV using the zero trans model (n = 8–9 sections/region/treatment). (f) SalA (10 μM)- and 16-BrSalA (5, 10 μM)-induced change in uptake of 10 μM ASP+ in HEK-293 cells coexpressing the DAT and KOR as well as the effect of pretreatment with nor-BNI (1 μM) or U0126 (20 μM) (n = 38–65 cells/treatment). ** p < 0.01, **** p < 0.0001 (Dunnett’s test). # p < 0.05, ## p < 0.01 (Tukey test).
PMC10304272
molecules-28-04848-g004.jpg
0.468573
9ccfacca8cbc4d49b2483726803c4cfb
Effect of 16-BrSalA compared to SalA and other KOR agonists in various preclinical side effect assays. (a) Time spent on the open arms of the elevated plus maze as a function of vehicle, SalA (0.3, 1.0 mg/kg), or 16-BrSalA (1.0 mg/kg) treatment (n = 15–29/treatment). (b) Time spent in the light box in the light–dark test (with an inset showing the total distance travelled) as a function of vehicle, SalA (0.3, 1.0 mg/kg), or 16-BrSalA (1.0 mg/kg) treatment (n = 9–24/treatment). (c) Mobility and immobility time in the forced swim test as a function of vehicle or 16-BrSalA (1.0 mg/kg) treatment (8–11/treatment). (d) Total active lever responses maintained by sucrose reinforcement following vehicle, U50,488 (10.0 mg/kg), U69,593 (0.3 mg/kg), SalA (0.3 mg/kg), or 16-BrSalA (1.0 mg/kg) treatment (n = 7). (e) Recognition index in the novel object recognition test as a function of vehicle, U50,488 (10.0 mg/kg), SalA (0.3, 1.0 mg/kg), or 16-BrSalA (1.0 mg/kg) treatment (n = 21). (f) Percentage of time spent in the paired chamber pre- and postconditioning with the vehicle, SalA (0.3), or 16-BrSalA (1.0 mg/kg) (n = 8–11/treatment). * p < 0.05, ** p < 0.01, *** p < 0.001, and **** p < 0.0001 (Dunnett’s [a–e] and Šídák’s [f] tests). Note that the data from the control groups have been published previously [65].
PMC10304272
molecules-28-04848-g005.jpg
0.498344
f8842c0b671f41d688e803caeb634b04
Effect of 16-BrSalA on extracellular-signal-regulated kinases 1 and 2 (ERK1/2) and p38. Phosphorylated ERK1/2 (p-ERK1/2) was quantified in rat NAcc (a), dSTR (b), and prefrontal cortex (PFC) (c) tissue collected 0–120 min following treatment with 16-BrSalA (n = 5–7/timepoint/region). (d) p-ERK1/2 was also measured in HEK-293 cells coexpressing the DAT and KOR 0–180 min following treatment with 16-BrSalA (n = 8 dishes/timepoint). (e) Effect of treatment with nor-BNI (1 μM) or U0126 (20 μM) on 16-brSalA (10 μM)-induced ERK1/2 expression in HEK-293 cells coexpressing DAT and KOR (n = 7–8 dishes/treatment). Phosphorylated p38 (p-p38) was similarly measured in rat NAcc (f), dSTR (g), and PFC (h) tissue collected 0–120 min following treatment with 16-BrSalA (n = 5–7/timepoint/region). Representative Western blot scans are displayed above each graph. p-ERK1/2 and p-p38 expression were normalized to total ERK1/2 and p38, respectively, and expressed as fold change from the baseline (time 0/vehicle + vehicle treatment). * p < 0.05, ** p < 0.01, and **** p < 0.0001 compared to the baseline (one-sample t-test).
PMC10304272
molecules-28-04848-g006.jpg
0.458857
ed2c468cf35e49dabfd80adf3cb1f230
Effect of 16-BrSalA, SalA, and nalorphine in rhesus monkeys (n = 3). (a) Cumulative dose effect of 16-BrSalA, SalA, nalorphine, and vehicle on mean (±SEM) serum levels of prolactin. Prolactin levels are expressed as change from pre-injection values (Δng/mL). (b) Time course of mean (±SEM) blood prolactin levels following 16-BrSalA administration (0.056 mg/kg, i.v.). (c) Cumulative dose effect of 16-BrSalA and SalA on the median sedation score.
PMC10304272
molecules-28-04848-g007.jpg
0.372207
4b4740c200554aa7a028a93976bd8342
Development of the risk nomogram (A) and the dynamic nomogram for an example (B). The nomogram to predict the risk of diabetic foot in patients with T2DM was developed with predictors including age, history of smoking, HbA1C, WBC, and LDL-C. Smoking history, 1; no smoking history, 0.
PMC10304289
fendo-14-1186992-g001.jpg
0.46424
4175bfd455c748e5ade4829469ad3a18
The accuracy of the nomogram for predicting DF using the ROC curve, the area under the ROC curve AUC represents the ability of the model to distinguish between DF and simple type 2 diabetes. When AUC = 0.5 said that the probability of the model distinguishing diabetic foot patients was 50%, it indicated that the probability of the model distinguishing diabetic foot patients was 100%. The closer the AUC was to 1, the stronger the distinguishing ability of the model was. The area under the ROC curve of the training set and verification set of the model (AUC) was 0.827 (A) and 0.808 (B), respectively. It showed that the model has good distinguishing ability.
PMC10304289
fendo-14-1186992-g002.jpg
0.399986
ff180a638ee348918d50dfa41a40b304
Calibration curves of the DF risk in nomogram prediction. The thick dotted line represents an ideal prediction, and the thin dotted line represents the predictive ability of the nomogram. The closer the thick dotted line fit is to the thin dotted line, the better the predictive accuracy of the nomogram is. The calibration curves of the training set (A) and the verification set (B) have good agreement between the prediction probability and the actual probability, and the average absolute errors are all less than 0.05.
PMC10304289
fendo-14-1186992-g003.jpg
0.390321
2cd39c03ad054d129c8d85b9ec4f9655
Decision curve analysis for the DF risk nomogram. The y-axis measured the net benefit. The green dotted line represented the assumption that all patients had DF. The blue dotted line represented the assumption that all patients had no DF. The solid red line represented the risk nomogram. The threshold ranges of the nomogram model in the training set (A) and verification set (B) are 0.10 to 0.85 and 0.10 to 0.75, respectively, indicating that the model has a wide range of safety and high clinical practical value.
PMC10304289
fendo-14-1186992-g004.jpg
0.467335
dfa3d238b9bc4276b961d2e65a093bcf
Maps showing the sampling locations in Ningbo and photographs of the sampling sites. (A) Portion of the map of China showing the location of Ningbo. (B) Map showing the sampling locations in Meishan Wetlands (colored square indicates the sampling sites). (C) Sampling site for Pleuronema ningboensis n. sp. (D) Sampling site for Pleuronema orientale.
PMC10304819
microorganisms-11-01422-g001.jpg
0.472826
b8d058f75fd546a481d885398ea71706
Pleuronema ningboensis n. sp. in vivo (A) and after protargol staining (B–D). (A) Left ventral view of a typical individual; arrowhead shows the contractile vacuole, arrow points to caudal cilia. (B) Detail of oral apparatus. (C,D) Left ventrolateral (C) and right dorsolateral (D) views to show the ciliature. M1, membranelle 1; M2a, membranelle 2a; M2b, membranelle 2b; M3, membranelle 3; PM, paroral membrane; PK, paroral kineties; Ma, macronucleus. Scale bars = 30 μm (A,C,D); 20 μm (B).
PMC10304819
microorganisms-11-01422-g002.jpg
0.42839
b68a56f885bb4c77b8db6838dd84b7ff
Pleuronema ningboensis n. sp. from life (A–E) and after protargol impregnation (F–J). (A) Left ventrolateral view of a typical individual in vivo; arrow indicates the grooves on the cell surface. (B,C) different individuals, showing the variation in cell view; arrowhead in (C) indicates the contractile vacuole. (D) Detail of the cortex; arrowhead indicates extrusomes. (E) Posterior portion of cell; arrowhead points to caudal cilia. (F) Detail of ciliature, arrowheads mark the posterior end of preoral kineties. (G) Anterior portion of the oral apparatus, arrowhead points to paroral membrane, arrow points to membranelle 2a. (H) To show membranelle 1 (arrow). (I) Posterior portion of the oral apparatus, to show membranelle 2b (arrow) and membranelle 3 (arrowhead indicates the leftmost row, double arrowheads indicate the rightmost row). (J) Left ventral view of the holotype specimen. Ma, macronucleus. Scale bars = 30 μm.
PMC10304819
microorganisms-11-01422-g003.jpg
0.43326
89d058773b284844a7d0239021cc4ccf
Pleuronema orientale from life (A) and after protargol impregnation (B–G). (A) Left ventral view of a representative individual, arrow indicates caudal cilia; arrowhead indicates contractile vacuole. (B) Detail of the oral apparatus. (C–E) To show the different numbers of macronuclei in different individuals; arrowheads indicate macronuclei. (F,G) Left ventrolateral (F) and right dorsolateral (G) views to show ciliature and macronucleus. M1, membranelle 1; M2a, membranelle 2a; M2b, membranelle 2b; M3, membranelle 3; PM, paroral membrane; PK, paroral kineties; Ma, macronucleus. Scale bars = 50 μm (A,C–G); 30 μm (B).
PMC10304819
microorganisms-11-01422-g004.jpg
0.394571
4b0dba8d80654cd1bf675f122c9cfaee
Photomicrographs of Pleuronema orientale in vivo (A–D) and after protargol staining (E–K). (A–C) Left ventrolateral views of different individuals; arrowhead in (A) marks caudal cilia, arrowheads in (B,C) point to contractile vacuole. (D) To show the detail of paroral membrane (arrowhead). (E,H) To show different numbers of preoral kineties (arrowheads). (F) Detail of double macronuclei. (G) To show membranelle 1 (arrow). (I) To show three-rowed membranelle 3 (arrow). (J) To show membranelle 2a (arrow), membranelle 2b (arrowhead), and paroral membrane (double arrowheads). (K) Left ventrolateral view of the holotype specimen. Ma, macronucleus. Scale bars = 50 μm.
PMC10304819
microorganisms-11-01422-g005.jpg
0.430437
24a074bd6d68454680b27d0a873becde
Maximum likelihood (ML) tree based on SSU rDNA sequences showing positions of Pleuronema ningboensis n. sp. and the Ningbo population of Pleuronema orientale (sequences in red). Numbers at nodes indicate the bootstrap values of maximum likelihood (ML) out of 1000 replicates and the posterior probabilities of Bayesian analysis (BI). Solid circles represent full bootstrap supports from both algorithms. Asterisks (*) indicate a mismatch in topologies between ML and BI analyses. The scale bar corresponds to five substitutions per 100 nucleotide positions. All branches are drawn to scale. The systematic classification mainly follows Gao et al. [28]. Pleuronema coronatum (JX310014) (marked with red triangle) deviates from the other three P. coronatum sequences; unfortunately, available information is not sufficient to determine its identity. Pleuronema clades of ‘coronatum-type’ and ‘marinum-type’ groups are marked with yellow and blue background colors, respectively.
PMC10304819
microorganisms-11-01422-g006.jpg
0.405963
2f5fe9a12d164bd99aa7cd09c9067e44
Sequence comparison of the SSU rDNA showing the unmatched nucleotides between Pleuronema ningboensis n. sp. and its most closely related congeners. (A) Nucleotide positions are given at the top of each column. Matched sites are represented by dots (·). (B) Matrix showing the percentage of sequence identity (below the diagonal) and the number of unmatched nucleotides (above the diagonal).
PMC10304819
microorganisms-11-01422-g007.jpg
0.525109
8e2b7de45a724d5598128c1425d01604
The three-wall defect of the first molar in SD rats. (a) The surgical field on the rat skull, indicating the selected sample area that was sectioned. (b) Three-wall defect procedures were performed rostral to the upper first molar using a 1.4 mm round tungsten carbide drill. The blue arrow and dotted circle on the left indicate intact bone, while the red arrow and dotted circle on the right indicate the postoperative bone defect, measuring 2 × 1.4 × 1.4 mm³. Note that the dash-dotted line in (b) indicates the histological section.
PMC10305005
polymers-15-02649-g001.jpg
0.429038
0e5fb1460be44867b173f4a3fecf7279
Anatomical reference and calculation method. The cementum–enamel junction and bone height were chosen as anatomical references for the assessment of epithelial downgrowth and residual bone defects, and tooth width and tooth height were also considered to correct for deviations in sample section angles. In addition, the angle of the epi-tooth axis was also evaluated.
PMC10305005
polymers-15-02649-g002.jpg
0.437354
8445bd27518e4302a6ebc55b66ab6f7b
HPLF-laden collagen scaffolds constructed from dental LEDs. (a) Strain test (%) of collagen structures cured with the dental LED at different times. The stiffness of the 3 s/9 s/21 s group was higher than that of the 0 s group (*** p < 0.001). (b) The cell viability under the LED light for 3 s was higher than that of the 9 s and 21 s groups (** p < 0.01). (c–e) Collagen cell scaffolds were illuminated with the dental LED for 3 s/9 s/21 s and cell viability was tested by Live-Dead (bar: 100 µm).
PMC10305005
polymers-15-02649-g003.jpg
0.448906
47642cf4d7e44d53a8c973adb1a45853
Immunofluorescence staining of α-SMA and ALP in the collagen scaffolds laden with HPLFs (n = 2 for each 1, 7, 14 day experiment). (a) Relative fluorescence intensity of DAPI, α-SMA/DAPI, and ALP/DAPI at 1, 7, 14 days (n = 6). (b) On the 7th day, both α-SMA and ALP expression were observed, indicating the differentiation of HPLF cells into myofibroblasts and pre-fibroblasts (bar: 100 µm). (* p < 0.05 and ** p < 0.01, compared with D1; # p < 0.05 significant between groups).
PMC10305005
polymers-15-02649-g004.jpg
0.42207
343ba109d55c46879cbab8d0b1021325
Histology of epithelium healing and bone growth are shown. Movement of the junctional epithelium is a key factor in the progression of periodontitis. The smaller residual bone defect and well-oriented periodontal ligaments indicated better postoperative periodontal regeneration capability. (a–c) Blank, (d–f) COL_LED, (g–i) COL_HPLF, (j–l) COL_HPLF_LED. (b,e,h,k) are enlarged pictures from the small box in (a,d,g,j), respectively. The 0° direction represents the orientation of the periodontal ligament fibers that are parallel to the cemento-enamel junction (CEJ) of the tooth. A higher peak at 0° indicates well-organized periodontal ligament fibers. In the COL_HPLF_LED group, osteoblasts aggregated near the newly formed bone, and the periodontal ligament was oriented in connection with the bone and root surfaces. This finding indicated that the periodontal ligament was effectively restored and functioned well in the COL_HPLF_LED group. (Arrows indicate periodontal ligaments, and arrowheads indicate osteoblasts accumulation.)
PMC10305005
polymers-15-02649-g005.jpg
0.413024
1775f6a0ae404d8389461753f050b5ee
Combination of HPLF cells with LED-illuminated collagen scaffolds promotes periodontal tissue regeneration (Y-axis unit: %). Both (a) epithelial downgrowth and (b) relative epithelial downgrowth were significantly reduced in the COL_HPLF_LED group compared to the Blank group (** p < 0.01) and the COL_LED group (# p < 0.05, ## p < 0.01). Similarly, (c) residual bone defect and (d) relative residual bone defect were significantly reduced in the COL_HPLF_LED group compared to the Blank group (* p < 0.05) and the COL_LED group (# p < 0.05). (e) Compared with the COL_LED group, the angle between the epithelium and the tooth axis was significantly reduced in the COL_HPLF_LED group (# p < 0.05).
PMC10305005
polymers-15-02649-g006.jpg
0.595222
b218dd2056684fe59f2eadc1e8d5646a
Cyclic voltammograms of 1 mmol L−1 K3[Fe(CN)6] in 1 mol L−1 KCl for CB/GC electrode (blue line), TiO2/GC electrode (green line) and for CB/TiO2/GC electrode (red line) with a scan rate of 100 mV s−1.
PMC10305077
sensors-23-05397-g001.jpg
0.400877
91e9e16fd7d54b7688cceb1e242365bf
Nyquist plot obtained in 1 mmol L−1 K3[Fe(CN)6] in 1 mol L−1 KCl for GC electrode (black) CB/GC electrode (blue), TiO2/GC electrode (green), and for CB/TiO2/GC electrode (red).
PMC10305077
sensors-23-05397-g002.jpg
0.422562
8e1aa18ba5624215a369b5e7d241585f
Cyclic voltammograms of 1 μmol L−1 sumatriptan measured in a 0.1 mol L−1 phosphate buffer (pH 6.0) on the CB-TiO2/GC electrode. The scan rate values were as follows: 6.3; 12.5; 25; 50; 100; 200; 250; and 500 mV s−1.
PMC10305077
sensors-23-05397-g003.jpg
0.423845
c949708fab3e4972b6239696de705d15
Plots of the sumatriptan peak current and potential dependence on the supporting electrolyte pH in the range 4.6–9.1 (A) and corresponding SWV voltammograms of 0.5 μmol L−1 sumatriptan for pH in the range 5.5–8.0 measured in a 0.1 mol L−1 phosphate buffer (B). Instrumental parameters as in point 2.6.
PMC10305077
sensors-23-05397-g004.jpg
0.459
b5468227567d42fcad1f419c28735cf6
Comparison of voltammograms obtained for the anodic peak of 1 μmol L−1 sumatriptan, measured in the 0.1 mol L−1 phosphate buffer (pH 6.0) on the surface of glassy carbon electrode (black line), glassy carbon electrode modified with carbon black (blue line), glassy carbon electrode modified with titanium dioxide (green line), and glassy carbon electrode modified with carbon black/titanium dioxide (red line) before and after background correction. Instrumental parameters as in point 2.6.
PMC10305077
sensors-23-05397-g005.jpg
0.524021
4f055c9ddfd84d4587a6a321aef1afae
Dependence of the sumatriptan peak current on the value of preconcentration time. SUM concentrations as follows: (a) 0.5 μmol L−1; (b) 0.05 μmol L−1; (c) 0.01 μmol L−1, and (d) 5 nmol L−1. Other instrumental parameters are the same as in point 2.6.
PMC10305077
sensors-23-05397-g006.jpg
0.438209
70adc586c37f4c6a9699761408d15221
SWV sumatriptan calibration curves registered for the preconcentration times (a) 10 s; (b) 45 s; (c) 150 s in 0.1 mol L−1 phosphate buffer (pH 6.0) (A) and corresponding voltammograms obtained for preconcentration time 45 s in the concentration range of 10–150 nmol L−1 (B). Other instrumental parameters are the same as in point 2.6.
PMC10305077
sensors-23-05397-g007.jpg
0.492841
6f49cc6583ef42f3b266e4caa00e53c0
Sumatriptan calibration plots in the concentration range from 1 to 5 μmol L−1 on modified SPCE in the amperometric parameters of measurements (A,B) and in the voltammetric parameters of measurements (C,D). All experiments were performed under flow injection conditions.
PMC10305077
sensors-23-05397-g008.jpg
0.535201
30274d97707f4780b2ec144cfa14e7cc
Possible scheme of sumatriptan oxidation of the glassy carbon electrode modified with carbon black and titanium dioxide suspension.
PMC10305077
sensors-23-05397-sch001.jpg
0.472765
2a7d18949c15483a81e332814a9cadef
Visualization of the RSS-based clustering. Excerpts show single likelihoods over a floorplan; overall graphic shows a combined likelihood from a set of 15 nodes.
PMC10305585
sensors-23-05772-g001.jpg
0.460983
dc90ae6990a149daa43c44f7c4a1cee5
Scenario 6.1 and 6.2. Results for synthetic scenario in single position with random clusters and schematic plot for clusters of size 1 and size 2 with variable distances.
PMC10305585
sensors-23-05772-g002.jpg
0.426674
aa92e5772aec42eeb8b38d2eade1ec4b
Scenario 6.2: Results for synthetic scenario for clusters of size N = 2 with fixed distance in a circle around node l′.
PMC10305585
sensors-23-05772-g003.jpg
0.450716
e5c7389365144dd68bad5f7219e3bf2d
Scenario 6.3: Results for synthetic scenario with genie-aided clusters (minimum distance to node 1).
PMC10305585
sensors-23-05772-g004.jpg
0.54114
935a165889694166ba9998de9582798d
Scenario 6.3: Floorplans with results for synthetic scenario with genie-aided clusters (showing only every 10th processed node for better visibility).
PMC10305585
sensors-23-05772-g005.jpg
0.482424
c8079630844b448faf3db97cc53e7e94
Scenario 6.4: Results for synthetic scenario with RSS-based clusters.
PMC10305585
sensors-23-05772-g006.jpg
0.493068
7c0b1ea274724173a997f78d845db9bb
Scenario 6.4: Floorplans with results for synthetic scenario with RSS-based clusters (showing only every 10th processed node for better visibility).
PMC10305585
sensors-23-05772-g007.jpg
0.446855
2e1356acc8e84ed494344ac935f67a11
Scenario 6.4 and 6.5: Pictures of the measurement setup and room. (a) Red ellipse marking one agent node. (b) Red ellipse marking the access point antenna for controlling the agent nodes. (c) Red ellipse marking one linear 2-antenna array. (d) Red ellipse marking the PC for measurement processing.
PMC10305585
sensors-23-05772-g008.jpg
0.464426
dad93ff008504ed099b9da1cb688ab6a
Scenario 6.5: Results for measurement scenario.
PMC10305585
sensors-23-05772-g009.jpg
0.54405
3ce504c30fd8493f9673c93cfc66c6fb
Scenario 6.5: Floorplans with results from measurements with RSS-based clusters (showing only every 10th processed node for better visibility).
PMC10305585
sensors-23-05772-g010.jpg
0.449616
c96498dc6b7d4f56b6b15568d7d88373
Scenario 6.5: CF plot and excerpt for different cluster sizes and cluster selection strategies.
PMC10305585
sensors-23-05772-g011.jpg
0.448022
3c05bf7bf2f74e38ba0e9b2ccb968efa
Illustration of fiber push-out experiment. Broken fiber–matrix interface partially relaxes residual stress in nearby matrix.
PMC10305614
polymers-15-02596-g001.jpg
0.453984
ac3fb38057284a3d9941a56fb33d447b
Nanoindenter measuring matrix sink-in: (a) before fiber push-out; (b) after fiber push-out; (c) load-displacement data showing local matrix sink-in after push-out experiment.
PMC10305614
polymers-15-02596-g002.jpg
0.442049
5fc562d385264932bae3912bddffb271
Flowchart of FEMU inverse algorithm calculating material parameters.
PMC10305614
polymers-15-02596-g003.jpg
0.408592
52c7d864545b486c9cd98e0d19e8c1bc
(a) Schematic of fiber push-out experiment. (b) Push-out specimen in SEM.
PMC10305614
polymers-15-02596-g004.jpg
0.43096
6ae53c008bcf4a5ab2f038ec15dda92d
Process of creating 3D FE Model based on microscopy data. (a) Detected fibers in blue. (b) Generated fibers in brown and inner zone boundary in black. (c) 3D FE Model, top view. (d) 3D FE Model, oblique view.
PMC10305614
polymers-15-02596-g005.jpg
0.44607
c2261859be8e4cda94fa18138c307738
Example of one FEM simulation. Epoxy-curing shrinkage generates through-thickness stress. Through-thickness membrane sink-in deformation is clearly shown after fiber push-out.
PMC10305614
polymers-15-02596-g006.jpg
0.520539
49293db0811e48a2a88b343b40303f2a
(a) FE model before push-out: red nodes corresponding to probed area. (b) FE model after push-out. (c) Z-displacement of probed area showing local matrix sink-in after push-out experiment.
PMC10305614
polymers-15-02596-g007.jpg
0.44864
98690821d5414856856a621b223ad4bc
Data from virtual curing experiments and their fits using resulting Kamal equation model. (a) Cases with temperature ramps. (b) Cases with isotherms.
PMC10305614
polymers-15-02596-g008.jpg
0.488622
6bae8e95e6e34beb88ddfaebb52b8002
Cure cycle, DOC evolution, and modulus development for F3G epoxy.
PMC10305614
polymers-15-02596-g009.jpg
0.451004
16ede723ae8845f1947be889d9f46451
SEM images and FE geometries of experimental samples analyzed with FEMU.
PMC10305614
polymers-15-02596-g010.jpg
0.468878
eaa8361c65eb43c9806a93916e987178
SEM image of Sample 5, Area 1, showing extensive matrix damage.
PMC10305614
polymers-15-02596-g011.jpg
0.463314
9b5a539284fd47058018d2bb6e7542e6
Before push-out: normal stress Szz in the fiber direction. Only matrix part is shown.
PMC10305614
polymers-15-02596-g012.jpg