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PMC9433320_Fig4_390339.jpg | Can you identify the primary element in this image? | Microtubules push liquified chromatin away from the spindle pole.a, Time-lapse microscopy analysis of liquified chromatin during monopolar spindle assembly. AluI was injected into live mitotic HeLa cells expressing H2B–mCherry and meGFP–CENP-A, stained with SiR–tubulin, in the presence of nocodazole (noco) and STLC. Nocodazole was then removed at t = 0 min during time-lapse imaging to induce monopolar spindle assembly. Projection of 5 z-sections. Time is shown as min:s. b, Quantification of bulk chromatin (H2B–mCherry) and centromeric chromatin (meGFP–CENP-A) localizing at the cell periphery relative to the region around the spindle monopole at t = 36 min. n = 15 cells. The bars indicate the mean. Significance was tested by a two-tailed Mann–Whitney U-test (P = 1.289 × 10−8). c, Model of chromatin compaction and condensin-mediated DNA looping in mitotic chromosome and spindle assembly. The illustration shows a top-down view of a chromosome cross-section. Biological replicates: n = 3 (a,b). Scale bars, 5 µm.Source Data |
PMC9433320_Fig4_390336.jpg | What key item or scene is captured in this photo? | Microtubules push liquified chromatin away from the spindle pole.a, Time-lapse microscopy analysis of liquified chromatin during monopolar spindle assembly. AluI was injected into live mitotic HeLa cells expressing H2B–mCherry and meGFP–CENP-A, stained with SiR–tubulin, in the presence of nocodazole (noco) and STLC. Nocodazole was then removed at t = 0 min during time-lapse imaging to induce monopolar spindle assembly. Projection of 5 z-sections. Time is shown as min:s. b, Quantification of bulk chromatin (H2B–mCherry) and centromeric chromatin (meGFP–CENP-A) localizing at the cell periphery relative to the region around the spindle monopole at t = 36 min. n = 15 cells. The bars indicate the mean. Significance was tested by a two-tailed Mann–Whitney U-test (P = 1.289 × 10−8). c, Model of chromatin compaction and condensin-mediated DNA looping in mitotic chromosome and spindle assembly. The illustration shows a top-down view of a chromosome cross-section. Biological replicates: n = 3 (a,b). Scale bars, 5 µm.Source Data |
PMC9433320_Fig4_390342.jpg | What is being portrayed in this visual content? | Microtubules push liquified chromatin away from the spindle pole.a, Time-lapse microscopy analysis of liquified chromatin during monopolar spindle assembly. AluI was injected into live mitotic HeLa cells expressing H2B–mCherry and meGFP–CENP-A, stained with SiR–tubulin, in the presence of nocodazole (noco) and STLC. Nocodazole was then removed at t = 0 min during time-lapse imaging to induce monopolar spindle assembly. Projection of 5 z-sections. Time is shown as min:s. b, Quantification of bulk chromatin (H2B–mCherry) and centromeric chromatin (meGFP–CENP-A) localizing at the cell periphery relative to the region around the spindle monopole at t = 36 min. n = 15 cells. The bars indicate the mean. Significance was tested by a two-tailed Mann–Whitney U-test (P = 1.289 × 10−8). c, Model of chromatin compaction and condensin-mediated DNA looping in mitotic chromosome and spindle assembly. The illustration shows a top-down view of a chromosome cross-section. Biological replicates: n = 3 (a,b). Scale bars, 5 µm.Source Data |
PMC9433320_Fig6_390371.jpg | What is the main focus of this visual representation? | Chromosome and spindle organization in condensin-depleted and TSA-treated cells entering mitosis.a–c, 3D confocal time-lapse microscopy of live HeLa cells entering mitosis in the presence of STLC to induce monopolar spindle geometry. Cells have homozygous Smc4-mAID-Halo alleles and stably express OsTIR(F74G), H2B-mCherry, and CENP-A-meGFP and are stained with SiR-tubulin. Images show projection of 2 Z-sections with Z-offset of 2.5 µm, centred around the spindle pole. a, Mitotic entry of a control cell. b, Cell entering mitosis after treatment with 5-PhIAA to deplete Smc4 (ΔCondensin). c, Cell entering mitosis after treatment with 5-PhIAA to deplete Smc4 and TSA to suppress mitotic histone deacetylation (ΔCondensin+TSA). d, Quantification of chromatin distribution relative to spindle monopole of cells in a-c 20 min after prophase onset. Total H2B-mCherry fluorescence in pole-proximal region divided by total H2B-mCherry-fluorescence of pole-distal regions. n = 42 cells for control, n = 38 for ΔCondensin, n = 60 for ΔCondensin+TSA. Bars indicate mean, significance was tested by a two-tailed Mann-Whitney test (ΔCondensin, P = 0.988; ΔCondensin+TSA, P<10−15, precision limit of floating-point arithmetic). e, Quantification of fraction of kinetochores outside chromatin (>0.5 µm distance from chromatin surface) of cells as in a–c 20 min after prophase onset. n = 42 cells for control, n = 42 for ΔCondensin, n = 62 for ΔCondensin+TSA. Bars indicate mean, significance was tested by a two-tailed Mann-Whitney test (ΔCondensin, P<10−15, precision limit of floating-point arithmetic; ΔCondensin+TSA, P = 0.061). Biological replicates: n = 2 (a,c–e); n = 3 (b). Scale bars, 10 µm.
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PMC9433320_Fig6_390370.jpg | What is shown in this image? | Chromosome and spindle organization in condensin-depleted and TSA-treated cells entering mitosis.a–c, 3D confocal time-lapse microscopy of live HeLa cells entering mitosis in the presence of STLC to induce monopolar spindle geometry. Cells have homozygous Smc4-mAID-Halo alleles and stably express OsTIR(F74G), H2B-mCherry, and CENP-A-meGFP and are stained with SiR-tubulin. Images show projection of 2 Z-sections with Z-offset of 2.5 µm, centred around the spindle pole. a, Mitotic entry of a control cell. b, Cell entering mitosis after treatment with 5-PhIAA to deplete Smc4 (ΔCondensin). c, Cell entering mitosis after treatment with 5-PhIAA to deplete Smc4 and TSA to suppress mitotic histone deacetylation (ΔCondensin+TSA). d, Quantification of chromatin distribution relative to spindle monopole of cells in a-c 20 min after prophase onset. Total H2B-mCherry fluorescence in pole-proximal region divided by total H2B-mCherry-fluorescence of pole-distal regions. n = 42 cells for control, n = 38 for ΔCondensin, n = 60 for ΔCondensin+TSA. Bars indicate mean, significance was tested by a two-tailed Mann-Whitney test (ΔCondensin, P = 0.988; ΔCondensin+TSA, P<10−15, precision limit of floating-point arithmetic). e, Quantification of fraction of kinetochores outside chromatin (>0.5 µm distance from chromatin surface) of cells as in a–c 20 min after prophase onset. n = 42 cells for control, n = 42 for ΔCondensin, n = 62 for ΔCondensin+TSA. Bars indicate mean, significance was tested by a two-tailed Mann-Whitney test (ΔCondensin, P<10−15, precision limit of floating-point arithmetic; ΔCondensin+TSA, P = 0.061). Biological replicates: n = 2 (a,c–e); n = 3 (b). Scale bars, 10 µm.
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PMC9433320_Fig10_390382.jpg | Describe the main subject of this image. | Correlative fluorescence and electron microscopy of mitotic cells and analysis of chromosome segregation by live imaging after TSA treatment.a, b, Electron tomograms of prometaphase WT Hela cells, untreated (a) or treated with TSA (b). Magenta: chromatin surfaces; green: microtubules in cytoplasm; cyan: microtubules in chromatin; red circles: microtubule perforation sites at chromosome surface. Representative example regions for control prometaphase (n = 3), control metaphase (n = 7), TSA prometaphase (n = 5) and TSA metaphase (n = 5); example regions are from 10 tomograms per condition from 7 different cells each. c, Quantification of microtubule density in chromatin regions of prometaphase or metaphase cells in the absence or presence of TSA. Data shown in Fig.1e, f separated by mitotic stage, n = 10 tomograms from 7 different cells for each condition. Bars indicate mean. d, Correlative transmission electron microscopy and fluorescence microscopy of chromatin/H2B-mCherry in prometaphase WT Hela cells (related to a, control cell #1 and b, TSA cell #1). e, Mitotic progression analysis by time-lapse microscopy of HeLa cells expressing H2B-mRFP, in untreated control and TSA-treated cells. n = 44 for control from 5 biological replicates, n = 36 for TSA from 4 biological replicates. Time is relative to nuclear envelope disassembly (NEBD). f, Chromosome missegregation analysis by Airyscan imaging of live anaphase HeLa cells expressing H2B-mCherry and meGFP-CENP-A and stained with SiR-tubulin. Representative images of n=64 control cells and n = 110 TSA-treated cells. Single Z-sections. g, Quantification of chromosome missegregation of cells as illustrated in f. Dots indicate biological replicates, bars indicate mean. n=64 cells for control, n = 110 for TSA. h, Quantification of number of lagging chromosomes in cells as illustrated in f. Fraction of cells with 1, 2, or 3 lagging chromosomes. n = 64 cells for control, n = 110 for TSA. Biological replicates: n = 2 (a–h). Scale bars, a,b, 250 nm section, 2 µm; tomogram slices and 3D model, 200 nm; d, 2 µm; f, 5 µm.
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PMC9433320_Fig10_390389.jpg | What is the core subject represented in this visual? | Correlative fluorescence and electron microscopy of mitotic cells and analysis of chromosome segregation by live imaging after TSA treatment.a, b, Electron tomograms of prometaphase WT Hela cells, untreated (a) or treated with TSA (b). Magenta: chromatin surfaces; green: microtubules in cytoplasm; cyan: microtubules in chromatin; red circles: microtubule perforation sites at chromosome surface. Representative example regions for control prometaphase (n = 3), control metaphase (n = 7), TSA prometaphase (n = 5) and TSA metaphase (n = 5); example regions are from 10 tomograms per condition from 7 different cells each. c, Quantification of microtubule density in chromatin regions of prometaphase or metaphase cells in the absence or presence of TSA. Data shown in Fig.1e, f separated by mitotic stage, n = 10 tomograms from 7 different cells for each condition. Bars indicate mean. d, Correlative transmission electron microscopy and fluorescence microscopy of chromatin/H2B-mCherry in prometaphase WT Hela cells (related to a, control cell #1 and b, TSA cell #1). e, Mitotic progression analysis by time-lapse microscopy of HeLa cells expressing H2B-mRFP, in untreated control and TSA-treated cells. n = 44 for control from 5 biological replicates, n = 36 for TSA from 4 biological replicates. Time is relative to nuclear envelope disassembly (NEBD). f, Chromosome missegregation analysis by Airyscan imaging of live anaphase HeLa cells expressing H2B-mCherry and meGFP-CENP-A and stained with SiR-tubulin. Representative images of n=64 control cells and n = 110 TSA-treated cells. Single Z-sections. g, Quantification of chromosome missegregation of cells as illustrated in f. Dots indicate biological replicates, bars indicate mean. n=64 cells for control, n = 110 for TSA. h, Quantification of number of lagging chromosomes in cells as illustrated in f. Fraction of cells with 1, 2, or 3 lagging chromosomes. n = 64 cells for control, n = 110 for TSA. Biological replicates: n = 2 (a–h). Scale bars, a,b, 250 nm section, 2 µm; tomogram slices and 3D model, 200 nm; d, 2 µm; f, 5 µm.
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PMC9433320_Fig10_390387.jpg | What is being portrayed in this visual content? | Correlative fluorescence and electron microscopy of mitotic cells and analysis of chromosome segregation by live imaging after TSA treatment.a, b, Electron tomograms of prometaphase WT Hela cells, untreated (a) or treated with TSA (b). Magenta: chromatin surfaces; green: microtubules in cytoplasm; cyan: microtubules in chromatin; red circles: microtubule perforation sites at chromosome surface. Representative example regions for control prometaphase (n = 3), control metaphase (n = 7), TSA prometaphase (n = 5) and TSA metaphase (n = 5); example regions are from 10 tomograms per condition from 7 different cells each. c, Quantification of microtubule density in chromatin regions of prometaphase or metaphase cells in the absence or presence of TSA. Data shown in Fig.1e, f separated by mitotic stage, n = 10 tomograms from 7 different cells for each condition. Bars indicate mean. d, Correlative transmission electron microscopy and fluorescence microscopy of chromatin/H2B-mCherry in prometaphase WT Hela cells (related to a, control cell #1 and b, TSA cell #1). e, Mitotic progression analysis by time-lapse microscopy of HeLa cells expressing H2B-mRFP, in untreated control and TSA-treated cells. n = 44 for control from 5 biological replicates, n = 36 for TSA from 4 biological replicates. Time is relative to nuclear envelope disassembly (NEBD). f, Chromosome missegregation analysis by Airyscan imaging of live anaphase HeLa cells expressing H2B-mCherry and meGFP-CENP-A and stained with SiR-tubulin. Representative images of n=64 control cells and n = 110 TSA-treated cells. Single Z-sections. g, Quantification of chromosome missegregation of cells as illustrated in f. Dots indicate biological replicates, bars indicate mean. n=64 cells for control, n = 110 for TSA. h, Quantification of number of lagging chromosomes in cells as illustrated in f. Fraction of cells with 1, 2, or 3 lagging chromosomes. n = 64 cells for control, n = 110 for TSA. Biological replicates: n = 2 (a–h). Scale bars, a,b, 250 nm section, 2 µm; tomogram slices and 3D model, 200 nm; d, 2 µm; f, 5 µm.
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PMC9433320_Fig10_390381.jpg | What key item or scene is captured in this photo? | Correlative fluorescence and electron microscopy of mitotic cells and analysis of chromosome segregation by live imaging after TSA treatment.a, b, Electron tomograms of prometaphase WT Hela cells, untreated (a) or treated with TSA (b). Magenta: chromatin surfaces; green: microtubules in cytoplasm; cyan: microtubules in chromatin; red circles: microtubule perforation sites at chromosome surface. Representative example regions for control prometaphase (n = 3), control metaphase (n = 7), TSA prometaphase (n = 5) and TSA metaphase (n = 5); example regions are from 10 tomograms per condition from 7 different cells each. c, Quantification of microtubule density in chromatin regions of prometaphase or metaphase cells in the absence or presence of TSA. Data shown in Fig.1e, f separated by mitotic stage, n = 10 tomograms from 7 different cells for each condition. Bars indicate mean. d, Correlative transmission electron microscopy and fluorescence microscopy of chromatin/H2B-mCherry in prometaphase WT Hela cells (related to a, control cell #1 and b, TSA cell #1). e, Mitotic progression analysis by time-lapse microscopy of HeLa cells expressing H2B-mRFP, in untreated control and TSA-treated cells. n = 44 for control from 5 biological replicates, n = 36 for TSA from 4 biological replicates. Time is relative to nuclear envelope disassembly (NEBD). f, Chromosome missegregation analysis by Airyscan imaging of live anaphase HeLa cells expressing H2B-mCherry and meGFP-CENP-A and stained with SiR-tubulin. Representative images of n=64 control cells and n = 110 TSA-treated cells. Single Z-sections. g, Quantification of chromosome missegregation of cells as illustrated in f. Dots indicate biological replicates, bars indicate mean. n=64 cells for control, n = 110 for TSA. h, Quantification of number of lagging chromosomes in cells as illustrated in f. Fraction of cells with 1, 2, or 3 lagging chromosomes. n = 64 cells for control, n = 110 for TSA. Biological replicates: n = 2 (a–h). Scale bars, a,b, 250 nm section, 2 µm; tomogram slices and 3D model, 200 nm; d, 2 µm; f, 5 µm.
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PMC9433320_Fig10_390373.jpg | What is the focal point of this photograph? | Correlative fluorescence and electron microscopy of mitotic cells and analysis of chromosome segregation by live imaging after TSA treatment.a, b, Electron tomograms of prometaphase WT Hela cells, untreated (a) or treated with TSA (b). Magenta: chromatin surfaces; green: microtubules in cytoplasm; cyan: microtubules in chromatin; red circles: microtubule perforation sites at chromosome surface. Representative example regions for control prometaphase (n = 3), control metaphase (n = 7), TSA prometaphase (n = 5) and TSA metaphase (n = 5); example regions are from 10 tomograms per condition from 7 different cells each. c, Quantification of microtubule density in chromatin regions of prometaphase or metaphase cells in the absence or presence of TSA. Data shown in Fig.1e, f separated by mitotic stage, n = 10 tomograms from 7 different cells for each condition. Bars indicate mean. d, Correlative transmission electron microscopy and fluorescence microscopy of chromatin/H2B-mCherry in prometaphase WT Hela cells (related to a, control cell #1 and b, TSA cell #1). e, Mitotic progression analysis by time-lapse microscopy of HeLa cells expressing H2B-mRFP, in untreated control and TSA-treated cells. n = 44 for control from 5 biological replicates, n = 36 for TSA from 4 biological replicates. Time is relative to nuclear envelope disassembly (NEBD). f, Chromosome missegregation analysis by Airyscan imaging of live anaphase HeLa cells expressing H2B-mCherry and meGFP-CENP-A and stained with SiR-tubulin. Representative images of n=64 control cells and n = 110 TSA-treated cells. Single Z-sections. g, Quantification of chromosome missegregation of cells as illustrated in f. Dots indicate biological replicates, bars indicate mean. n=64 cells for control, n = 110 for TSA. h, Quantification of number of lagging chromosomes in cells as illustrated in f. Fraction of cells with 1, 2, or 3 lagging chromosomes. n = 64 cells for control, n = 110 for TSA. Biological replicates: n = 2 (a–h). Scale bars, a,b, 250 nm section, 2 µm; tomogram slices and 3D model, 200 nm; d, 2 µm; f, 5 µm.
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PMC9433320_Fig10_390374.jpg | What is the focal point of this photograph? | Correlative fluorescence and electron microscopy of mitotic cells and analysis of chromosome segregation by live imaging after TSA treatment.a, b, Electron tomograms of prometaphase WT Hela cells, untreated (a) or treated with TSA (b). Magenta: chromatin surfaces; green: microtubules in cytoplasm; cyan: microtubules in chromatin; red circles: microtubule perforation sites at chromosome surface. Representative example regions for control prometaphase (n = 3), control metaphase (n = 7), TSA prometaphase (n = 5) and TSA metaphase (n = 5); example regions are from 10 tomograms per condition from 7 different cells each. c, Quantification of microtubule density in chromatin regions of prometaphase or metaphase cells in the absence or presence of TSA. Data shown in Fig.1e, f separated by mitotic stage, n = 10 tomograms from 7 different cells for each condition. Bars indicate mean. d, Correlative transmission electron microscopy and fluorescence microscopy of chromatin/H2B-mCherry in prometaphase WT Hela cells (related to a, control cell #1 and b, TSA cell #1). e, Mitotic progression analysis by time-lapse microscopy of HeLa cells expressing H2B-mRFP, in untreated control and TSA-treated cells. n = 44 for control from 5 biological replicates, n = 36 for TSA from 4 biological replicates. Time is relative to nuclear envelope disassembly (NEBD). f, Chromosome missegregation analysis by Airyscan imaging of live anaphase HeLa cells expressing H2B-mCherry and meGFP-CENP-A and stained with SiR-tubulin. Representative images of n=64 control cells and n = 110 TSA-treated cells. Single Z-sections. g, Quantification of chromosome missegregation of cells as illustrated in f. Dots indicate biological replicates, bars indicate mean. n=64 cells for control, n = 110 for TSA. h, Quantification of number of lagging chromosomes in cells as illustrated in f. Fraction of cells with 1, 2, or 3 lagging chromosomes. n = 64 cells for control, n = 110 for TSA. Biological replicates: n = 2 (a–h). Scale bars, a,b, 250 nm section, 2 µm; tomogram slices and 3D model, 200 nm; d, 2 µm; f, 5 µm.
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PMC9433320_Fig10_390378.jpg | What is the core subject represented in this visual? | Correlative fluorescence and electron microscopy of mitotic cells and analysis of chromosome segregation by live imaging after TSA treatment.a, b, Electron tomograms of prometaphase WT Hela cells, untreated (a) or treated with TSA (b). Magenta: chromatin surfaces; green: microtubules in cytoplasm; cyan: microtubules in chromatin; red circles: microtubule perforation sites at chromosome surface. Representative example regions for control prometaphase (n = 3), control metaphase (n = 7), TSA prometaphase (n = 5) and TSA metaphase (n = 5); example regions are from 10 tomograms per condition from 7 different cells each. c, Quantification of microtubule density in chromatin regions of prometaphase or metaphase cells in the absence or presence of TSA. Data shown in Fig.1e, f separated by mitotic stage, n = 10 tomograms from 7 different cells for each condition. Bars indicate mean. d, Correlative transmission electron microscopy and fluorescence microscopy of chromatin/H2B-mCherry in prometaphase WT Hela cells (related to a, control cell #1 and b, TSA cell #1). e, Mitotic progression analysis by time-lapse microscopy of HeLa cells expressing H2B-mRFP, in untreated control and TSA-treated cells. n = 44 for control from 5 biological replicates, n = 36 for TSA from 4 biological replicates. Time is relative to nuclear envelope disassembly (NEBD). f, Chromosome missegregation analysis by Airyscan imaging of live anaphase HeLa cells expressing H2B-mCherry and meGFP-CENP-A and stained with SiR-tubulin. Representative images of n=64 control cells and n = 110 TSA-treated cells. Single Z-sections. g, Quantification of chromosome missegregation of cells as illustrated in f. Dots indicate biological replicates, bars indicate mean. n=64 cells for control, n = 110 for TSA. h, Quantification of number of lagging chromosomes in cells as illustrated in f. Fraction of cells with 1, 2, or 3 lagging chromosomes. n = 64 cells for control, n = 110 for TSA. Biological replicates: n = 2 (a–h). Scale bars, a,b, 250 nm section, 2 µm; tomogram slices and 3D model, 200 nm; d, 2 µm; f, 5 µm.
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PMC9433320_Fig10_390376.jpg | Can you identify the primary element in this image? | Correlative fluorescence and electron microscopy of mitotic cells and analysis of chromosome segregation by live imaging after TSA treatment.a, b, Electron tomograms of prometaphase WT Hela cells, untreated (a) or treated with TSA (b). Magenta: chromatin surfaces; green: microtubules in cytoplasm; cyan: microtubules in chromatin; red circles: microtubule perforation sites at chromosome surface. Representative example regions for control prometaphase (n = 3), control metaphase (n = 7), TSA prometaphase (n = 5) and TSA metaphase (n = 5); example regions are from 10 tomograms per condition from 7 different cells each. c, Quantification of microtubule density in chromatin regions of prometaphase or metaphase cells in the absence or presence of TSA. Data shown in Fig.1e, f separated by mitotic stage, n = 10 tomograms from 7 different cells for each condition. Bars indicate mean. d, Correlative transmission electron microscopy and fluorescence microscopy of chromatin/H2B-mCherry in prometaphase WT Hela cells (related to a, control cell #1 and b, TSA cell #1). e, Mitotic progression analysis by time-lapse microscopy of HeLa cells expressing H2B-mRFP, in untreated control and TSA-treated cells. n = 44 for control from 5 biological replicates, n = 36 for TSA from 4 biological replicates. Time is relative to nuclear envelope disassembly (NEBD). f, Chromosome missegregation analysis by Airyscan imaging of live anaphase HeLa cells expressing H2B-mCherry and meGFP-CENP-A and stained with SiR-tubulin. Representative images of n=64 control cells and n = 110 TSA-treated cells. Single Z-sections. g, Quantification of chromosome missegregation of cells as illustrated in f. Dots indicate biological replicates, bars indicate mean. n=64 cells for control, n = 110 for TSA. h, Quantification of number of lagging chromosomes in cells as illustrated in f. Fraction of cells with 1, 2, or 3 lagging chromosomes. n = 64 cells for control, n = 110 for TSA. Biological replicates: n = 2 (a–h). Scale bars, a,b, 250 nm section, 2 µm; tomogram slices and 3D model, 200 nm; d, 2 µm; f, 5 µm.
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PMC9433320_Fig10_390380.jpg | What is being portrayed in this visual content? | Correlative fluorescence and electron microscopy of mitotic cells and analysis of chromosome segregation by live imaging after TSA treatment.a, b, Electron tomograms of prometaphase WT Hela cells, untreated (a) or treated with TSA (b). Magenta: chromatin surfaces; green: microtubules in cytoplasm; cyan: microtubules in chromatin; red circles: microtubule perforation sites at chromosome surface. Representative example regions for control prometaphase (n = 3), control metaphase (n = 7), TSA prometaphase (n = 5) and TSA metaphase (n = 5); example regions are from 10 tomograms per condition from 7 different cells each. c, Quantification of microtubule density in chromatin regions of prometaphase or metaphase cells in the absence or presence of TSA. Data shown in Fig.1e, f separated by mitotic stage, n = 10 tomograms from 7 different cells for each condition. Bars indicate mean. d, Correlative transmission electron microscopy and fluorescence microscopy of chromatin/H2B-mCherry in prometaphase WT Hela cells (related to a, control cell #1 and b, TSA cell #1). e, Mitotic progression analysis by time-lapse microscopy of HeLa cells expressing H2B-mRFP, in untreated control and TSA-treated cells. n = 44 for control from 5 biological replicates, n = 36 for TSA from 4 biological replicates. Time is relative to nuclear envelope disassembly (NEBD). f, Chromosome missegregation analysis by Airyscan imaging of live anaphase HeLa cells expressing H2B-mCherry and meGFP-CENP-A and stained with SiR-tubulin. Representative images of n=64 control cells and n = 110 TSA-treated cells. Single Z-sections. g, Quantification of chromosome missegregation of cells as illustrated in f. Dots indicate biological replicates, bars indicate mean. n=64 cells for control, n = 110 for TSA. h, Quantification of number of lagging chromosomes in cells as illustrated in f. Fraction of cells with 1, 2, or 3 lagging chromosomes. n = 64 cells for control, n = 110 for TSA. Biological replicates: n = 2 (a–h). Scale bars, a,b, 250 nm section, 2 µm; tomogram slices and 3D model, 200 nm; d, 2 µm; f, 5 µm.
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PMC9433320_Fig10_390377.jpg | Can you identify the primary element in this image? | Correlative fluorescence and electron microscopy of mitotic cells and analysis of chromosome segregation by live imaging after TSA treatment.a, b, Electron tomograms of prometaphase WT Hela cells, untreated (a) or treated with TSA (b). Magenta: chromatin surfaces; green: microtubules in cytoplasm; cyan: microtubules in chromatin; red circles: microtubule perforation sites at chromosome surface. Representative example regions for control prometaphase (n = 3), control metaphase (n = 7), TSA prometaphase (n = 5) and TSA metaphase (n = 5); example regions are from 10 tomograms per condition from 7 different cells each. c, Quantification of microtubule density in chromatin regions of prometaphase or metaphase cells in the absence or presence of TSA. Data shown in Fig.1e, f separated by mitotic stage, n = 10 tomograms from 7 different cells for each condition. Bars indicate mean. d, Correlative transmission electron microscopy and fluorescence microscopy of chromatin/H2B-mCherry in prometaphase WT Hela cells (related to a, control cell #1 and b, TSA cell #1). e, Mitotic progression analysis by time-lapse microscopy of HeLa cells expressing H2B-mRFP, in untreated control and TSA-treated cells. n = 44 for control from 5 biological replicates, n = 36 for TSA from 4 biological replicates. Time is relative to nuclear envelope disassembly (NEBD). f, Chromosome missegregation analysis by Airyscan imaging of live anaphase HeLa cells expressing H2B-mCherry and meGFP-CENP-A and stained with SiR-tubulin. Representative images of n=64 control cells and n = 110 TSA-treated cells. Single Z-sections. g, Quantification of chromosome missegregation of cells as illustrated in f. Dots indicate biological replicates, bars indicate mean. n=64 cells for control, n = 110 for TSA. h, Quantification of number of lagging chromosomes in cells as illustrated in f. Fraction of cells with 1, 2, or 3 lagging chromosomes. n = 64 cells for control, n = 110 for TSA. Biological replicates: n = 2 (a–h). Scale bars, a,b, 250 nm section, 2 µm; tomogram slices and 3D model, 200 nm; d, 2 µm; f, 5 µm.
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PMC9433320_Fig10_390388.jpg | Describe the main subject of this image. | Correlative fluorescence and electron microscopy of mitotic cells and analysis of chromosome segregation by live imaging after TSA treatment.a, b, Electron tomograms of prometaphase WT Hela cells, untreated (a) or treated with TSA (b). Magenta: chromatin surfaces; green: microtubules in cytoplasm; cyan: microtubules in chromatin; red circles: microtubule perforation sites at chromosome surface. Representative example regions for control prometaphase (n = 3), control metaphase (n = 7), TSA prometaphase (n = 5) and TSA metaphase (n = 5); example regions are from 10 tomograms per condition from 7 different cells each. c, Quantification of microtubule density in chromatin regions of prometaphase or metaphase cells in the absence or presence of TSA. Data shown in Fig.1e, f separated by mitotic stage, n = 10 tomograms from 7 different cells for each condition. Bars indicate mean. d, Correlative transmission electron microscopy and fluorescence microscopy of chromatin/H2B-mCherry in prometaphase WT Hela cells (related to a, control cell #1 and b, TSA cell #1). e, Mitotic progression analysis by time-lapse microscopy of HeLa cells expressing H2B-mRFP, in untreated control and TSA-treated cells. n = 44 for control from 5 biological replicates, n = 36 for TSA from 4 biological replicates. Time is relative to nuclear envelope disassembly (NEBD). f, Chromosome missegregation analysis by Airyscan imaging of live anaphase HeLa cells expressing H2B-mCherry and meGFP-CENP-A and stained with SiR-tubulin. Representative images of n=64 control cells and n = 110 TSA-treated cells. Single Z-sections. g, Quantification of chromosome missegregation of cells as illustrated in f. Dots indicate biological replicates, bars indicate mean. n=64 cells for control, n = 110 for TSA. h, Quantification of number of lagging chromosomes in cells as illustrated in f. Fraction of cells with 1, 2, or 3 lagging chromosomes. n = 64 cells for control, n = 110 for TSA. Biological replicates: n = 2 (a–h). Scale bars, a,b, 250 nm section, 2 µm; tomogram slices and 3D model, 200 nm; d, 2 µm; f, 5 µm.
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PMC9433320_Fig10_390375.jpg | What can you see in this picture? | Correlative fluorescence and electron microscopy of mitotic cells and analysis of chromosome segregation by live imaging after TSA treatment.a, b, Electron tomograms of prometaphase WT Hela cells, untreated (a) or treated with TSA (b). Magenta: chromatin surfaces; green: microtubules in cytoplasm; cyan: microtubules in chromatin; red circles: microtubule perforation sites at chromosome surface. Representative example regions for control prometaphase (n = 3), control metaphase (n = 7), TSA prometaphase (n = 5) and TSA metaphase (n = 5); example regions are from 10 tomograms per condition from 7 different cells each. c, Quantification of microtubule density in chromatin regions of prometaphase or metaphase cells in the absence or presence of TSA. Data shown in Fig.1e, f separated by mitotic stage, n = 10 tomograms from 7 different cells for each condition. Bars indicate mean. d, Correlative transmission electron microscopy and fluorescence microscopy of chromatin/H2B-mCherry in prometaphase WT Hela cells (related to a, control cell #1 and b, TSA cell #1). e, Mitotic progression analysis by time-lapse microscopy of HeLa cells expressing H2B-mRFP, in untreated control and TSA-treated cells. n = 44 for control from 5 biological replicates, n = 36 for TSA from 4 biological replicates. Time is relative to nuclear envelope disassembly (NEBD). f, Chromosome missegregation analysis by Airyscan imaging of live anaphase HeLa cells expressing H2B-mCherry and meGFP-CENP-A and stained with SiR-tubulin. Representative images of n=64 control cells and n = 110 TSA-treated cells. Single Z-sections. g, Quantification of chromosome missegregation of cells as illustrated in f. Dots indicate biological replicates, bars indicate mean. n=64 cells for control, n = 110 for TSA. h, Quantification of number of lagging chromosomes in cells as illustrated in f. Fraction of cells with 1, 2, or 3 lagging chromosomes. n = 64 cells for control, n = 110 for TSA. Biological replicates: n = 2 (a–h). Scale bars, a,b, 250 nm section, 2 µm; tomogram slices and 3D model, 200 nm; d, 2 µm; f, 5 µm.
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PMC9433320_Fig14_390392.jpg | What does this image primarily show? | Microtubules push liquified chromatin away from the spindle pole independently of hKid and Kif4A.a, Time-lapse microscopy of liquified chromatin during chemically-induced assembly of monopolar spindles. Live mitotic HeLa cells expressing H2B-mCherry and eGFP-a-tubulin were treated with nocodazole and STLC and then injected with AluI. Nocodazole was removed at t = 0 min during time-lapse imaging. Projection of 4 z-sections. Representative example of n = 13 cells. b, Quantification of chromatin localization at the cell periphery relative to the region around the spindle monopole at = 36 min for cells as shown in a. c, Time-lapse microscopy of liquified chromatin during spindle assembly as in a for cells depleted of Kid and Kif4a by RNAi. d, Quantification as in b for hKid/Kif4a-RNAi cells. n = 16 cells. Bar indicates mean; significance tested by a two-tailed Mann-Whitney test (P = 0.215) e, Validation of RNAi efficiency by Western Blotting. Samples were collected 30 h after transfection of siRNAs targeting hKid and Kif4a and probed by antibodies as indicated; n = 2 experiments. For gel source data, see Supplementary Figure 1b. Biological replicates: n = 4 (a,b); n = 3 (c,d); n = 2 (e). Scale bars, 5 µm.
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PMC9433320_Fig14_390393.jpg | What is the core subject represented in this visual? | Microtubules push liquified chromatin away from the spindle pole independently of hKid and Kif4A.a, Time-lapse microscopy of liquified chromatin during chemically-induced assembly of monopolar spindles. Live mitotic HeLa cells expressing H2B-mCherry and eGFP-a-tubulin were treated with nocodazole and STLC and then injected with AluI. Nocodazole was removed at t = 0 min during time-lapse imaging. Projection of 4 z-sections. Representative example of n = 13 cells. b, Quantification of chromatin localization at the cell periphery relative to the region around the spindle monopole at = 36 min for cells as shown in a. c, Time-lapse microscopy of liquified chromatin during spindle assembly as in a for cells depleted of Kid and Kif4a by RNAi. d, Quantification as in b for hKid/Kif4a-RNAi cells. n = 16 cells. Bar indicates mean; significance tested by a two-tailed Mann-Whitney test (P = 0.215) e, Validation of RNAi efficiency by Western Blotting. Samples were collected 30 h after transfection of siRNAs targeting hKid and Kif4a and probed by antibodies as indicated; n = 2 experiments. For gel source data, see Supplementary Figure 1b. Biological replicates: n = 4 (a,b); n = 3 (c,d); n = 2 (e). Scale bars, 5 µm.
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PMC9433320_Fig14_390404.jpg | What is shown in this image? | Microtubules push liquified chromatin away from the spindle pole independently of hKid and Kif4A.a, Time-lapse microscopy of liquified chromatin during chemically-induced assembly of monopolar spindles. Live mitotic HeLa cells expressing H2B-mCherry and eGFP-a-tubulin were treated with nocodazole and STLC and then injected with AluI. Nocodazole was removed at t = 0 min during time-lapse imaging. Projection of 4 z-sections. Representative example of n = 13 cells. b, Quantification of chromatin localization at the cell periphery relative to the region around the spindle monopole at = 36 min for cells as shown in a. c, Time-lapse microscopy of liquified chromatin during spindle assembly as in a for cells depleted of Kid and Kif4a by RNAi. d, Quantification as in b for hKid/Kif4a-RNAi cells. n = 16 cells. Bar indicates mean; significance tested by a two-tailed Mann-Whitney test (P = 0.215) e, Validation of RNAi efficiency by Western Blotting. Samples were collected 30 h after transfection of siRNAs targeting hKid and Kif4a and probed by antibodies as indicated; n = 2 experiments. For gel source data, see Supplementary Figure 1b. Biological replicates: n = 4 (a,b); n = 3 (c,d); n = 2 (e). Scale bars, 5 µm.
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PMC9433320_Fig14_390398.jpg | What is the main focus of this visual representation? | Microtubules push liquified chromatin away from the spindle pole independently of hKid and Kif4A.a, Time-lapse microscopy of liquified chromatin during chemically-induced assembly of monopolar spindles. Live mitotic HeLa cells expressing H2B-mCherry and eGFP-a-tubulin were treated with nocodazole and STLC and then injected with AluI. Nocodazole was removed at t = 0 min during time-lapse imaging. Projection of 4 z-sections. Representative example of n = 13 cells. b, Quantification of chromatin localization at the cell periphery relative to the region around the spindle monopole at = 36 min for cells as shown in a. c, Time-lapse microscopy of liquified chromatin during spindle assembly as in a for cells depleted of Kid and Kif4a by RNAi. d, Quantification as in b for hKid/Kif4a-RNAi cells. n = 16 cells. Bar indicates mean; significance tested by a two-tailed Mann-Whitney test (P = 0.215) e, Validation of RNAi efficiency by Western Blotting. Samples were collected 30 h after transfection of siRNAs targeting hKid and Kif4a and probed by antibodies as indicated; n = 2 experiments. For gel source data, see Supplementary Figure 1b. Biological replicates: n = 4 (a,b); n = 3 (c,d); n = 2 (e). Scale bars, 5 µm.
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PMC9433320_Fig14_390406.jpg | What is the principal component of this image? | Microtubules push liquified chromatin away from the spindle pole independently of hKid and Kif4A.a, Time-lapse microscopy of liquified chromatin during chemically-induced assembly of monopolar spindles. Live mitotic HeLa cells expressing H2B-mCherry and eGFP-a-tubulin were treated with nocodazole and STLC and then injected with AluI. Nocodazole was removed at t = 0 min during time-lapse imaging. Projection of 4 z-sections. Representative example of n = 13 cells. b, Quantification of chromatin localization at the cell periphery relative to the region around the spindle monopole at = 36 min for cells as shown in a. c, Time-lapse microscopy of liquified chromatin during spindle assembly as in a for cells depleted of Kid and Kif4a by RNAi. d, Quantification as in b for hKid/Kif4a-RNAi cells. n = 16 cells. Bar indicates mean; significance tested by a two-tailed Mann-Whitney test (P = 0.215) e, Validation of RNAi efficiency by Western Blotting. Samples were collected 30 h after transfection of siRNAs targeting hKid and Kif4a and probed by antibodies as indicated; n = 2 experiments. For gel source data, see Supplementary Figure 1b. Biological replicates: n = 4 (a,b); n = 3 (c,d); n = 2 (e). Scale bars, 5 µm.
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PMC9433320_Fig14_390397.jpg | What key item or scene is captured in this photo? | Microtubules push liquified chromatin away from the spindle pole independently of hKid and Kif4A.a, Time-lapse microscopy of liquified chromatin during chemically-induced assembly of monopolar spindles. Live mitotic HeLa cells expressing H2B-mCherry and eGFP-a-tubulin were treated with nocodazole and STLC and then injected with AluI. Nocodazole was removed at t = 0 min during time-lapse imaging. Projection of 4 z-sections. Representative example of n = 13 cells. b, Quantification of chromatin localization at the cell periphery relative to the region around the spindle monopole at = 36 min for cells as shown in a. c, Time-lapse microscopy of liquified chromatin during spindle assembly as in a for cells depleted of Kid and Kif4a by RNAi. d, Quantification as in b for hKid/Kif4a-RNAi cells. n = 16 cells. Bar indicates mean; significance tested by a two-tailed Mann-Whitney test (P = 0.215) e, Validation of RNAi efficiency by Western Blotting. Samples were collected 30 h after transfection of siRNAs targeting hKid and Kif4a and probed by antibodies as indicated; n = 2 experiments. For gel source data, see Supplementary Figure 1b. Biological replicates: n = 4 (a,b); n = 3 (c,d); n = 2 (e). Scale bars, 5 µm.
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PMC9433320_Fig14_390394.jpg | What is the main focus of this visual representation? | Microtubules push liquified chromatin away from the spindle pole independently of hKid and Kif4A.a, Time-lapse microscopy of liquified chromatin during chemically-induced assembly of monopolar spindles. Live mitotic HeLa cells expressing H2B-mCherry and eGFP-a-tubulin were treated with nocodazole and STLC and then injected with AluI. Nocodazole was removed at t = 0 min during time-lapse imaging. Projection of 4 z-sections. Representative example of n = 13 cells. b, Quantification of chromatin localization at the cell periphery relative to the region around the spindle monopole at = 36 min for cells as shown in a. c, Time-lapse microscopy of liquified chromatin during spindle assembly as in a for cells depleted of Kid and Kif4a by RNAi. d, Quantification as in b for hKid/Kif4a-RNAi cells. n = 16 cells. Bar indicates mean; significance tested by a two-tailed Mann-Whitney test (P = 0.215) e, Validation of RNAi efficiency by Western Blotting. Samples were collected 30 h after transfection of siRNAs targeting hKid and Kif4a and probed by antibodies as indicated; n = 2 experiments. For gel source data, see Supplementary Figure 1b. Biological replicates: n = 4 (a,b); n = 3 (c,d); n = 2 (e). Scale bars, 5 µm.
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PMC9433320_Fig14_390402.jpg | What stands out most in this visual? | Microtubules push liquified chromatin away from the spindle pole independently of hKid and Kif4A.a, Time-lapse microscopy of liquified chromatin during chemically-induced assembly of monopolar spindles. Live mitotic HeLa cells expressing H2B-mCherry and eGFP-a-tubulin were treated with nocodazole and STLC and then injected with AluI. Nocodazole was removed at t = 0 min during time-lapse imaging. Projection of 4 z-sections. Representative example of n = 13 cells. b, Quantification of chromatin localization at the cell periphery relative to the region around the spindle monopole at = 36 min for cells as shown in a. c, Time-lapse microscopy of liquified chromatin during spindle assembly as in a for cells depleted of Kid and Kif4a by RNAi. d, Quantification as in b for hKid/Kif4a-RNAi cells. n = 16 cells. Bar indicates mean; significance tested by a two-tailed Mann-Whitney test (P = 0.215) e, Validation of RNAi efficiency by Western Blotting. Samples were collected 30 h after transfection of siRNAs targeting hKid and Kif4a and probed by antibodies as indicated; n = 2 experiments. For gel source data, see Supplementary Figure 1b. Biological replicates: n = 4 (a,b); n = 3 (c,d); n = 2 (e). Scale bars, 5 µm.
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PMC9433320_Fig14_390391.jpg | What object or scene is depicted here? | Microtubules push liquified chromatin away from the spindle pole independently of hKid and Kif4A.a, Time-lapse microscopy of liquified chromatin during chemically-induced assembly of monopolar spindles. Live mitotic HeLa cells expressing H2B-mCherry and eGFP-a-tubulin were treated with nocodazole and STLC and then injected with AluI. Nocodazole was removed at t = 0 min during time-lapse imaging. Projection of 4 z-sections. Representative example of n = 13 cells. b, Quantification of chromatin localization at the cell periphery relative to the region around the spindle monopole at = 36 min for cells as shown in a. c, Time-lapse microscopy of liquified chromatin during spindle assembly as in a for cells depleted of Kid and Kif4a by RNAi. d, Quantification as in b for hKid/Kif4a-RNAi cells. n = 16 cells. Bar indicates mean; significance tested by a two-tailed Mann-Whitney test (P = 0.215) e, Validation of RNAi efficiency by Western Blotting. Samples were collected 30 h after transfection of siRNAs targeting hKid and Kif4a and probed by antibodies as indicated; n = 2 experiments. For gel source data, see Supplementary Figure 1b. Biological replicates: n = 4 (a,b); n = 3 (c,d); n = 2 (e). Scale bars, 5 µm.
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PMC9433320_Fig14_390390.jpg | What key item or scene is captured in this photo? | Microtubules push liquified chromatin away from the spindle pole independently of hKid and Kif4A.a, Time-lapse microscopy of liquified chromatin during chemically-induced assembly of monopolar spindles. Live mitotic HeLa cells expressing H2B-mCherry and eGFP-a-tubulin were treated with nocodazole and STLC and then injected with AluI. Nocodazole was removed at t = 0 min during time-lapse imaging. Projection of 4 z-sections. Representative example of n = 13 cells. b, Quantification of chromatin localization at the cell periphery relative to the region around the spindle monopole at = 36 min for cells as shown in a. c, Time-lapse microscopy of liquified chromatin during spindle assembly as in a for cells depleted of Kid and Kif4a by RNAi. d, Quantification as in b for hKid/Kif4a-RNAi cells. n = 16 cells. Bar indicates mean; significance tested by a two-tailed Mann-Whitney test (P = 0.215) e, Validation of RNAi efficiency by Western Blotting. Samples were collected 30 h after transfection of siRNAs targeting hKid and Kif4a and probed by antibodies as indicated; n = 2 experiments. For gel source data, see Supplementary Figure 1b. Biological replicates: n = 4 (a,b); n = 3 (c,d); n = 2 (e). Scale bars, 5 µm.
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PMC9433320_Fig14_390396.jpg | What is the central feature of this picture? | Microtubules push liquified chromatin away from the spindle pole independently of hKid and Kif4A.a, Time-lapse microscopy of liquified chromatin during chemically-induced assembly of monopolar spindles. Live mitotic HeLa cells expressing H2B-mCherry and eGFP-a-tubulin were treated with nocodazole and STLC and then injected with AluI. Nocodazole was removed at t = 0 min during time-lapse imaging. Projection of 4 z-sections. Representative example of n = 13 cells. b, Quantification of chromatin localization at the cell periphery relative to the region around the spindle monopole at = 36 min for cells as shown in a. c, Time-lapse microscopy of liquified chromatin during spindle assembly as in a for cells depleted of Kid and Kif4a by RNAi. d, Quantification as in b for hKid/Kif4a-RNAi cells. n = 16 cells. Bar indicates mean; significance tested by a two-tailed Mann-Whitney test (P = 0.215) e, Validation of RNAi efficiency by Western Blotting. Samples were collected 30 h after transfection of siRNAs targeting hKid and Kif4a and probed by antibodies as indicated; n = 2 experiments. For gel source data, see Supplementary Figure 1b. Biological replicates: n = 4 (a,b); n = 3 (c,d); n = 2 (e). Scale bars, 5 µm.
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PMC9433320_Fig14_390405.jpg | What object or scene is depicted here? | Microtubules push liquified chromatin away from the spindle pole independently of hKid and Kif4A.a, Time-lapse microscopy of liquified chromatin during chemically-induced assembly of monopolar spindles. Live mitotic HeLa cells expressing H2B-mCherry and eGFP-a-tubulin were treated with nocodazole and STLC and then injected with AluI. Nocodazole was removed at t = 0 min during time-lapse imaging. Projection of 4 z-sections. Representative example of n = 13 cells. b, Quantification of chromatin localization at the cell periphery relative to the region around the spindle monopole at = 36 min for cells as shown in a. c, Time-lapse microscopy of liquified chromatin during spindle assembly as in a for cells depleted of Kid and Kif4a by RNAi. d, Quantification as in b for hKid/Kif4a-RNAi cells. n = 16 cells. Bar indicates mean; significance tested by a two-tailed Mann-Whitney test (P = 0.215) e, Validation of RNAi efficiency by Western Blotting. Samples were collected 30 h after transfection of siRNAs targeting hKid and Kif4a and probed by antibodies as indicated; n = 2 experiments. For gel source data, see Supplementary Figure 1b. Biological replicates: n = 4 (a,b); n = 3 (c,d); n = 2 (e). Scale bars, 5 µm.
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PMC9433353_Fig1_390414.jpg | Can you identify the primary element in this image? | a-d, a preoperative view of the ACL tear, b preoperative view after DIS implantation, c preoperative MRI ACL tear, d postoperative MRI view with healed ACL (6 months) |
PMC9433353_Fig1_390417.jpg | What is the central feature of this picture? | a-d, a preoperative view of the ACL tear, b preoperative view after DIS implantation, c preoperative MRI ACL tear, d postoperative MRI view with healed ACL (6 months) |
PMC9433353_Fig1_390416.jpg | What's the most prominent thing you notice in this picture? | a-d, a preoperative view of the ACL tear, b preoperative view after DIS implantation, c preoperative MRI ACL tear, d postoperative MRI view with healed ACL (6 months) |
PMC9433353_Fig5_390419.jpg | What is the core subject represented in this visual? | a MRI view of ACL rupture, b arthroscopic view of the ACL tear, c arthroscopic view DIS repair, d MRI view of the non-healed ACL repair, e arthroscopic view of the non-healed ACL, f arthroscopic view of the notch after autograft ACL reconstruction |
PMC9433353_Fig5_390423.jpg | What does this image primarily show? | a MRI view of ACL rupture, b arthroscopic view of the ACL tear, c arthroscopic view DIS repair, d MRI view of the non-healed ACL repair, e arthroscopic view of the non-healed ACL, f arthroscopic view of the notch after autograft ACL reconstruction |
PMC9433353_Fig5_390422.jpg | What is the core subject represented in this visual? | a MRI view of ACL rupture, b arthroscopic view of the ACL tear, c arthroscopic view DIS repair, d MRI view of the non-healed ACL repair, e arthroscopic view of the non-healed ACL, f arthroscopic view of the notch after autograft ACL reconstruction |
PMC9433353_Fig5_390418.jpg | Describe the main subject of this image. | a MRI view of ACL rupture, b arthroscopic view of the ACL tear, c arthroscopic view DIS repair, d MRI view of the non-healed ACL repair, e arthroscopic view of the non-healed ACL, f arthroscopic view of the notch after autograft ACL reconstruction |
PMC9433353_Fig5_390420.jpg | Can you identify the primary element in this image? | a MRI view of ACL rupture, b arthroscopic view of the ACL tear, c arthroscopic view DIS repair, d MRI view of the non-healed ACL repair, e arthroscopic view of the non-healed ACL, f arthroscopic view of the notch after autograft ACL reconstruction |
PMC9433353_Fig5_390421.jpg | What object or scene is depicted here? | a MRI view of ACL rupture, b arthroscopic view of the ACL tear, c arthroscopic view DIS repair, d MRI view of the non-healed ACL repair, e arthroscopic view of the non-healed ACL, f arthroscopic view of the notch after autograft ACL reconstruction |
PMC9433422_f2_390424.jpg | What is being portrayed in this visual content? | Locations of manually and functionally defined ROIs. (A) Individual MNI locations. (B) Group mean MNI locations for four limbs. Please note that the locations were transformed to MNI brain only for illustration purposes, and all reported results are derived from the participants’ native space. (C) Depth of ROI locations shown with raincloud plots. MEG locations are shown to be situated deeper in the brain (i.e., furthest from the brain envelope) than fMRI and manual locations. Statistically significant differences are shown for each condition with * = P < 0.01 and ** = P < 0.001. LF = left finger, RF = right finger, LF = left ankle and RA = right ankle. |
PMC9433422_f2_390425.jpg | What is being portrayed in this visual content? | Locations of manually and functionally defined ROIs. (A) Individual MNI locations. (B) Group mean MNI locations for four limbs. Please note that the locations were transformed to MNI brain only for illustration purposes, and all reported results are derived from the participants’ native space. (C) Depth of ROI locations shown with raincloud plots. MEG locations are shown to be situated deeper in the brain (i.e., furthest from the brain envelope) than fMRI and manual locations. Statistically significant differences are shown for each condition with * = P < 0.01 and ** = P < 0.001. LF = left finger, RF = right finger, LF = left ankle and RA = right ankle. |
PMC9433422_f2_390427.jpg | What is the dominant medical problem in this image? | Locations of manually and functionally defined ROIs. (A) Individual MNI locations. (B) Group mean MNI locations for four limbs. Please note that the locations were transformed to MNI brain only for illustration purposes, and all reported results are derived from the participants’ native space. (C) Depth of ROI locations shown with raincloud plots. MEG locations are shown to be situated deeper in the brain (i.e., furthest from the brain envelope) than fMRI and manual locations. Statistically significant differences are shown for each condition with * = P < 0.01 and ** = P < 0.001. LF = left finger, RF = right finger, LF = left ankle and RA = right ankle. |
PMC9433422_f2_390428.jpg | What object or scene is depicted here? | Locations of manually and functionally defined ROIs. (A) Individual MNI locations. (B) Group mean MNI locations for four limbs. Please note that the locations were transformed to MNI brain only for illustration purposes, and all reported results are derived from the participants’ native space. (C) Depth of ROI locations shown with raincloud plots. MEG locations are shown to be situated deeper in the brain (i.e., furthest from the brain envelope) than fMRI and manual locations. Statistically significant differences are shown for each condition with * = P < 0.01 and ** = P < 0.001. LF = left finger, RF = right finger, LF = left ankle and RA = right ankle. |
PMC9433428_Fig8_390442.jpg | What is the central feature of this picture? | Depletion of USP49 impairs the tooth development.A Calcified teeth obtained after kidney capsule transplantation for 4 weeks. Three-dimensional (3D) reconstruction images from micro-computed tomography of calcified teeth in the (a) mock-group and (d) USP49-depletion group. H&E staining images from the (b, c) mock-group and (e, f) USP49-depletion group. Scale bar: 100 µm. (a (a, b)) In mock-group, the tooth is well calcified and surrounded by bone. (c) Pulp, odontoblasts, dentin, and ameloblasts were observed in the mock group. (a (d, e)) In the USP49-depletion group, the tooth is not fully calcified and not surrounded by bone. (f) Tooth showed defects in the pulp, dentin formation with few odontoblasts, and enamel formation with few ameloblasts. Fifteen biological replicates per group (n = 15) (H&E staining was performed from six biological replicates with randomly selected sections per group). B Amelogenin and DSPP expression levels in the (a and a’; b and b’) mock group and (c and c’, d and d’) USP49-depletion group. Scale bar = 100 µm. a’, b’, c’, and d’ are higher magnification images of a, b, c, and d, respectively. Ab, ameloblast; ES, enamel space; D, dentin; Ob, odontoblast; P, pulp; dotted line, dentinoenamel junction; arrows, positive cells in ameloblasts; arrow heads, positive cells in odontoblasts. The microscopic images from six biological replicates with randomly selected sections per group. |
PMC9433428_Fig8_390436.jpg | What is shown in this image? | Depletion of USP49 impairs the tooth development.A Calcified teeth obtained after kidney capsule transplantation for 4 weeks. Three-dimensional (3D) reconstruction images from micro-computed tomography of calcified teeth in the (a) mock-group and (d) USP49-depletion group. H&E staining images from the (b, c) mock-group and (e, f) USP49-depletion group. Scale bar: 100 µm. (a (a, b)) In mock-group, the tooth is well calcified and surrounded by bone. (c) Pulp, odontoblasts, dentin, and ameloblasts were observed in the mock group. (a (d, e)) In the USP49-depletion group, the tooth is not fully calcified and not surrounded by bone. (f) Tooth showed defects in the pulp, dentin formation with few odontoblasts, and enamel formation with few ameloblasts. Fifteen biological replicates per group (n = 15) (H&E staining was performed from six biological replicates with randomly selected sections per group). B Amelogenin and DSPP expression levels in the (a and a’; b and b’) mock group and (c and c’, d and d’) USP49-depletion group. Scale bar = 100 µm. a’, b’, c’, and d’ are higher magnification images of a, b, c, and d, respectively. Ab, ameloblast; ES, enamel space; D, dentin; Ob, odontoblast; P, pulp; dotted line, dentinoenamel junction; arrows, positive cells in ameloblasts; arrow heads, positive cells in odontoblasts. The microscopic images from six biological replicates with randomly selected sections per group. |
PMC9433428_Fig8_390441.jpg | Describe the main subject of this image. | Depletion of USP49 impairs the tooth development.A Calcified teeth obtained after kidney capsule transplantation for 4 weeks. Three-dimensional (3D) reconstruction images from micro-computed tomography of calcified teeth in the (a) mock-group and (d) USP49-depletion group. H&E staining images from the (b, c) mock-group and (e, f) USP49-depletion group. Scale bar: 100 µm. (a (a, b)) In mock-group, the tooth is well calcified and surrounded by bone. (c) Pulp, odontoblasts, dentin, and ameloblasts were observed in the mock group. (a (d, e)) In the USP49-depletion group, the tooth is not fully calcified and not surrounded by bone. (f) Tooth showed defects in the pulp, dentin formation with few odontoblasts, and enamel formation with few ameloblasts. Fifteen biological replicates per group (n = 15) (H&E staining was performed from six biological replicates with randomly selected sections per group). B Amelogenin and DSPP expression levels in the (a and a’; b and b’) mock group and (c and c’, d and d’) USP49-depletion group. Scale bar = 100 µm. a’, b’, c’, and d’ are higher magnification images of a, b, c, and d, respectively. Ab, ameloblast; ES, enamel space; D, dentin; Ob, odontoblast; P, pulp; dotted line, dentinoenamel junction; arrows, positive cells in ameloblasts; arrow heads, positive cells in odontoblasts. The microscopic images from six biological replicates with randomly selected sections per group. |
PMC9433428_Fig8_390430.jpg | What's the most prominent thing you notice in this picture? | Depletion of USP49 impairs the tooth development.A Calcified teeth obtained after kidney capsule transplantation for 4 weeks. Three-dimensional (3D) reconstruction images from micro-computed tomography of calcified teeth in the (a) mock-group and (d) USP49-depletion group. H&E staining images from the (b, c) mock-group and (e, f) USP49-depletion group. Scale bar: 100 µm. (a (a, b)) In mock-group, the tooth is well calcified and surrounded by bone. (c) Pulp, odontoblasts, dentin, and ameloblasts were observed in the mock group. (a (d, e)) In the USP49-depletion group, the tooth is not fully calcified and not surrounded by bone. (f) Tooth showed defects in the pulp, dentin formation with few odontoblasts, and enamel formation with few ameloblasts. Fifteen biological replicates per group (n = 15) (H&E staining was performed from six biological replicates with randomly selected sections per group). B Amelogenin and DSPP expression levels in the (a and a’; b and b’) mock group and (c and c’, d and d’) USP49-depletion group. Scale bar = 100 µm. a’, b’, c’, and d’ are higher magnification images of a, b, c, and d, respectively. Ab, ameloblast; ES, enamel space; D, dentin; Ob, odontoblast; P, pulp; dotted line, dentinoenamel junction; arrows, positive cells in ameloblasts; arrow heads, positive cells in odontoblasts. The microscopic images from six biological replicates with randomly selected sections per group. |
PMC9433428_Fig8_390433.jpg | What is the focal point of this photograph? | Depletion of USP49 impairs the tooth development.A Calcified teeth obtained after kidney capsule transplantation for 4 weeks. Three-dimensional (3D) reconstruction images from micro-computed tomography of calcified teeth in the (a) mock-group and (d) USP49-depletion group. H&E staining images from the (b, c) mock-group and (e, f) USP49-depletion group. Scale bar: 100 µm. (a (a, b)) In mock-group, the tooth is well calcified and surrounded by bone. (c) Pulp, odontoblasts, dentin, and ameloblasts were observed in the mock group. (a (d, e)) In the USP49-depletion group, the tooth is not fully calcified and not surrounded by bone. (f) Tooth showed defects in the pulp, dentin formation with few odontoblasts, and enamel formation with few ameloblasts. Fifteen biological replicates per group (n = 15) (H&E staining was performed from six biological replicates with randomly selected sections per group). B Amelogenin and DSPP expression levels in the (a and a’; b and b’) mock group and (c and c’, d and d’) USP49-depletion group. Scale bar = 100 µm. a’, b’, c’, and d’ are higher magnification images of a, b, c, and d, respectively. Ab, ameloblast; ES, enamel space; D, dentin; Ob, odontoblast; P, pulp; dotted line, dentinoenamel junction; arrows, positive cells in ameloblasts; arrow heads, positive cells in odontoblasts. The microscopic images from six biological replicates with randomly selected sections per group. |
PMC9433428_Fig8_390432.jpg | What is the central feature of this picture? | Depletion of USP49 impairs the tooth development.A Calcified teeth obtained after kidney capsule transplantation for 4 weeks. Three-dimensional (3D) reconstruction images from micro-computed tomography of calcified teeth in the (a) mock-group and (d) USP49-depletion group. H&E staining images from the (b, c) mock-group and (e, f) USP49-depletion group. Scale bar: 100 µm. (a (a, b)) In mock-group, the tooth is well calcified and surrounded by bone. (c) Pulp, odontoblasts, dentin, and ameloblasts were observed in the mock group. (a (d, e)) In the USP49-depletion group, the tooth is not fully calcified and not surrounded by bone. (f) Tooth showed defects in the pulp, dentin formation with few odontoblasts, and enamel formation with few ameloblasts. Fifteen biological replicates per group (n = 15) (H&E staining was performed from six biological replicates with randomly selected sections per group). B Amelogenin and DSPP expression levels in the (a and a’; b and b’) mock group and (c and c’, d and d’) USP49-depletion group. Scale bar = 100 µm. a’, b’, c’, and d’ are higher magnification images of a, b, c, and d, respectively. Ab, ameloblast; ES, enamel space; D, dentin; Ob, odontoblast; P, pulp; dotted line, dentinoenamel junction; arrows, positive cells in ameloblasts; arrow heads, positive cells in odontoblasts. The microscopic images from six biological replicates with randomly selected sections per group. |
PMC9433428_Fig8_390438.jpg | Can you identify the primary element in this image? | Depletion of USP49 impairs the tooth development.A Calcified teeth obtained after kidney capsule transplantation for 4 weeks. Three-dimensional (3D) reconstruction images from micro-computed tomography of calcified teeth in the (a) mock-group and (d) USP49-depletion group. H&E staining images from the (b, c) mock-group and (e, f) USP49-depletion group. Scale bar: 100 µm. (a (a, b)) In mock-group, the tooth is well calcified and surrounded by bone. (c) Pulp, odontoblasts, dentin, and ameloblasts were observed in the mock group. (a (d, e)) In the USP49-depletion group, the tooth is not fully calcified and not surrounded by bone. (f) Tooth showed defects in the pulp, dentin formation with few odontoblasts, and enamel formation with few ameloblasts. Fifteen biological replicates per group (n = 15) (H&E staining was performed from six biological replicates with randomly selected sections per group). B Amelogenin and DSPP expression levels in the (a and a’; b and b’) mock group and (c and c’, d and d’) USP49-depletion group. Scale bar = 100 µm. a’, b’, c’, and d’ are higher magnification images of a, b, c, and d, respectively. Ab, ameloblast; ES, enamel space; D, dentin; Ob, odontoblast; P, pulp; dotted line, dentinoenamel junction; arrows, positive cells in ameloblasts; arrow heads, positive cells in odontoblasts. The microscopic images from six biological replicates with randomly selected sections per group. |
PMC9433428_Fig8_390431.jpg | What is the core subject represented in this visual? | Depletion of USP49 impairs the tooth development.A Calcified teeth obtained after kidney capsule transplantation for 4 weeks. Three-dimensional (3D) reconstruction images from micro-computed tomography of calcified teeth in the (a) mock-group and (d) USP49-depletion group. H&E staining images from the (b, c) mock-group and (e, f) USP49-depletion group. Scale bar: 100 µm. (a (a, b)) In mock-group, the tooth is well calcified and surrounded by bone. (c) Pulp, odontoblasts, dentin, and ameloblasts were observed in the mock group. (a (d, e)) In the USP49-depletion group, the tooth is not fully calcified and not surrounded by bone. (f) Tooth showed defects in the pulp, dentin formation with few odontoblasts, and enamel formation with few ameloblasts. Fifteen biological replicates per group (n = 15) (H&E staining was performed from six biological replicates with randomly selected sections per group). B Amelogenin and DSPP expression levels in the (a and a’; b and b’) mock group and (c and c’, d and d’) USP49-depletion group. Scale bar = 100 µm. a’, b’, c’, and d’ are higher magnification images of a, b, c, and d, respectively. Ab, ameloblast; ES, enamel space; D, dentin; Ob, odontoblast; P, pulp; dotted line, dentinoenamel junction; arrows, positive cells in ameloblasts; arrow heads, positive cells in odontoblasts. The microscopic images from six biological replicates with randomly selected sections per group. |
PMC9433428_Fig8_390439.jpg | What's the most prominent thing you notice in this picture? | Depletion of USP49 impairs the tooth development.A Calcified teeth obtained after kidney capsule transplantation for 4 weeks. Three-dimensional (3D) reconstruction images from micro-computed tomography of calcified teeth in the (a) mock-group and (d) USP49-depletion group. H&E staining images from the (b, c) mock-group and (e, f) USP49-depletion group. Scale bar: 100 µm. (a (a, b)) In mock-group, the tooth is well calcified and surrounded by bone. (c) Pulp, odontoblasts, dentin, and ameloblasts were observed in the mock group. (a (d, e)) In the USP49-depletion group, the tooth is not fully calcified and not surrounded by bone. (f) Tooth showed defects in the pulp, dentin formation with few odontoblasts, and enamel formation with few ameloblasts. Fifteen biological replicates per group (n = 15) (H&E staining was performed from six biological replicates with randomly selected sections per group). B Amelogenin and DSPP expression levels in the (a and a’; b and b’) mock group and (c and c’, d and d’) USP49-depletion group. Scale bar = 100 µm. a’, b’, c’, and d’ are higher magnification images of a, b, c, and d, respectively. Ab, ameloblast; ES, enamel space; D, dentin; Ob, odontoblast; P, pulp; dotted line, dentinoenamel junction; arrows, positive cells in ameloblasts; arrow heads, positive cells in odontoblasts. The microscopic images from six biological replicates with randomly selected sections per group. |
PMC9433428_Fig8_390437.jpg | What object or scene is depicted here? | Depletion of USP49 impairs the tooth development.A Calcified teeth obtained after kidney capsule transplantation for 4 weeks. Three-dimensional (3D) reconstruction images from micro-computed tomography of calcified teeth in the (a) mock-group and (d) USP49-depletion group. H&E staining images from the (b, c) mock-group and (e, f) USP49-depletion group. Scale bar: 100 µm. (a (a, b)) In mock-group, the tooth is well calcified and surrounded by bone. (c) Pulp, odontoblasts, dentin, and ameloblasts were observed in the mock group. (a (d, e)) In the USP49-depletion group, the tooth is not fully calcified and not surrounded by bone. (f) Tooth showed defects in the pulp, dentin formation with few odontoblasts, and enamel formation with few ameloblasts. Fifteen biological replicates per group (n = 15) (H&E staining was performed from six biological replicates with randomly selected sections per group). B Amelogenin and DSPP expression levels in the (a and a’; b and b’) mock group and (c and c’, d and d’) USP49-depletion group. Scale bar = 100 µm. a’, b’, c’, and d’ are higher magnification images of a, b, c, and d, respectively. Ab, ameloblast; ES, enamel space; D, dentin; Ob, odontoblast; P, pulp; dotted line, dentinoenamel junction; arrows, positive cells in ameloblasts; arrow heads, positive cells in odontoblasts. The microscopic images from six biological replicates with randomly selected sections per group. |
PMC9433428_Fig8_390440.jpg | What is the principal component of this image? | Depletion of USP49 impairs the tooth development.A Calcified teeth obtained after kidney capsule transplantation for 4 weeks. Three-dimensional (3D) reconstruction images from micro-computed tomography of calcified teeth in the (a) mock-group and (d) USP49-depletion group. H&E staining images from the (b, c) mock-group and (e, f) USP49-depletion group. Scale bar: 100 µm. (a (a, b)) In mock-group, the tooth is well calcified and surrounded by bone. (c) Pulp, odontoblasts, dentin, and ameloblasts were observed in the mock group. (a (d, e)) In the USP49-depletion group, the tooth is not fully calcified and not surrounded by bone. (f) Tooth showed defects in the pulp, dentin formation with few odontoblasts, and enamel formation with few ameloblasts. Fifteen biological replicates per group (n = 15) (H&E staining was performed from six biological replicates with randomly selected sections per group). B Amelogenin and DSPP expression levels in the (a and a’; b and b’) mock group and (c and c’, d and d’) USP49-depletion group. Scale bar = 100 µm. a’, b’, c’, and d’ are higher magnification images of a, b, c, and d, respectively. Ab, ameloblast; ES, enamel space; D, dentin; Ob, odontoblast; P, pulp; dotted line, dentinoenamel junction; arrows, positive cells in ameloblasts; arrow heads, positive cells in odontoblasts. The microscopic images from six biological replicates with randomly selected sections per group. |
PMC9433428_Fig8_390443.jpg | What is the central feature of this picture? | Depletion of USP49 impairs the tooth development.A Calcified teeth obtained after kidney capsule transplantation for 4 weeks. Three-dimensional (3D) reconstruction images from micro-computed tomography of calcified teeth in the (a) mock-group and (d) USP49-depletion group. H&E staining images from the (b, c) mock-group and (e, f) USP49-depletion group. Scale bar: 100 µm. (a (a, b)) In mock-group, the tooth is well calcified and surrounded by bone. (c) Pulp, odontoblasts, dentin, and ameloblasts were observed in the mock group. (a (d, e)) In the USP49-depletion group, the tooth is not fully calcified and not surrounded by bone. (f) Tooth showed defects in the pulp, dentin formation with few odontoblasts, and enamel formation with few ameloblasts. Fifteen biological replicates per group (n = 15) (H&E staining was performed from six biological replicates with randomly selected sections per group). B Amelogenin and DSPP expression levels in the (a and a’; b and b’) mock group and (c and c’, d and d’) USP49-depletion group. Scale bar = 100 µm. a’, b’, c’, and d’ are higher magnification images of a, b, c, and d, respectively. Ab, ameloblast; ES, enamel space; D, dentin; Ob, odontoblast; P, pulp; dotted line, dentinoenamel junction; arrows, positive cells in ameloblasts; arrow heads, positive cells in odontoblasts. The microscopic images from six biological replicates with randomly selected sections per group. |
PMC9433541_f3_390459.jpg | What is being portrayed in this visual content? | Illustration of the biofilms of C. albicans and C. tropicalis at 48 and 72 h of growth by scanning electron microscopy using different magnifications (1.67, 3.33, 16.7, and 66.7 kx). Time samples of 48 and 72 h were used to compare the biofilm structure as well as cell morphology and area of the biofilms using a Tescan Mira 3 scanning electron microscope equipped with a Schottky field emission gun (Schottky FEG-SEM). Yeast cell area was calculated from the best pictures by Fiji ImageJ version 1.57 (Schindelin et al., 2012). |
PMC9433541_f3_390453.jpg | What is the central feature of this picture? | Illustration of the biofilms of C. albicans and C. tropicalis at 48 and 72 h of growth by scanning electron microscopy using different magnifications (1.67, 3.33, 16.7, and 66.7 kx). Time samples of 48 and 72 h were used to compare the biofilm structure as well as cell morphology and area of the biofilms using a Tescan Mira 3 scanning electron microscope equipped with a Schottky field emission gun (Schottky FEG-SEM). Yeast cell area was calculated from the best pictures by Fiji ImageJ version 1.57 (Schindelin et al., 2012). |
PMC9433541_f3_390444.jpg | What is the core subject represented in this visual? | Illustration of the biofilms of C. albicans and C. tropicalis at 48 and 72 h of growth by scanning electron microscopy using different magnifications (1.67, 3.33, 16.7, and 66.7 kx). Time samples of 48 and 72 h were used to compare the biofilm structure as well as cell morphology and area of the biofilms using a Tescan Mira 3 scanning electron microscope equipped with a Schottky field emission gun (Schottky FEG-SEM). Yeast cell area was calculated from the best pictures by Fiji ImageJ version 1.57 (Schindelin et al., 2012). |
PMC9433541_f3_390456.jpg | What is the main focus of this visual representation? | Illustration of the biofilms of C. albicans and C. tropicalis at 48 and 72 h of growth by scanning electron microscopy using different magnifications (1.67, 3.33, 16.7, and 66.7 kx). Time samples of 48 and 72 h were used to compare the biofilm structure as well as cell morphology and area of the biofilms using a Tescan Mira 3 scanning electron microscope equipped with a Schottky field emission gun (Schottky FEG-SEM). Yeast cell area was calculated from the best pictures by Fiji ImageJ version 1.57 (Schindelin et al., 2012). |
PMC9433541_f3_390449.jpg | What is the principal component of this image? | Illustration of the biofilms of C. albicans and C. tropicalis at 48 and 72 h of growth by scanning electron microscopy using different magnifications (1.67, 3.33, 16.7, and 66.7 kx). Time samples of 48 and 72 h were used to compare the biofilm structure as well as cell morphology and area of the biofilms using a Tescan Mira 3 scanning electron microscope equipped with a Schottky field emission gun (Schottky FEG-SEM). Yeast cell area was calculated from the best pictures by Fiji ImageJ version 1.57 (Schindelin et al., 2012). |
PMC9433541_f3_390447.jpg | What is the main focus of this visual representation? | Illustration of the biofilms of C. albicans and C. tropicalis at 48 and 72 h of growth by scanning electron microscopy using different magnifications (1.67, 3.33, 16.7, and 66.7 kx). Time samples of 48 and 72 h were used to compare the biofilm structure as well as cell morphology and area of the biofilms using a Tescan Mira 3 scanning electron microscope equipped with a Schottky field emission gun (Schottky FEG-SEM). Yeast cell area was calculated from the best pictures by Fiji ImageJ version 1.57 (Schindelin et al., 2012). |
PMC9433541_f3_390445.jpg | Can you identify the primary element in this image? | Illustration of the biofilms of C. albicans and C. tropicalis at 48 and 72 h of growth by scanning electron microscopy using different magnifications (1.67, 3.33, 16.7, and 66.7 kx). Time samples of 48 and 72 h were used to compare the biofilm structure as well as cell morphology and area of the biofilms using a Tescan Mira 3 scanning electron microscope equipped with a Schottky field emission gun (Schottky FEG-SEM). Yeast cell area was calculated from the best pictures by Fiji ImageJ version 1.57 (Schindelin et al., 2012). |
PMC9433541_f3_390450.jpg | Describe the main subject of this image. | Illustration of the biofilms of C. albicans and C. tropicalis at 48 and 72 h of growth by scanning electron microscopy using different magnifications (1.67, 3.33, 16.7, and 66.7 kx). Time samples of 48 and 72 h were used to compare the biofilm structure as well as cell morphology and area of the biofilms using a Tescan Mira 3 scanning electron microscope equipped with a Schottky field emission gun (Schottky FEG-SEM). Yeast cell area was calculated from the best pictures by Fiji ImageJ version 1.57 (Schindelin et al., 2012). |
PMC9433541_f3_390446.jpg | What key item or scene is captured in this photo? | Illustration of the biofilms of C. albicans and C. tropicalis at 48 and 72 h of growth by scanning electron microscopy using different magnifications (1.67, 3.33, 16.7, and 66.7 kx). Time samples of 48 and 72 h were used to compare the biofilm structure as well as cell morphology and area of the biofilms using a Tescan Mira 3 scanning electron microscope equipped with a Schottky field emission gun (Schottky FEG-SEM). Yeast cell area was calculated from the best pictures by Fiji ImageJ version 1.57 (Schindelin et al., 2012). |
PMC9433541_f3_390452.jpg | What object or scene is depicted here? | Illustration of the biofilms of C. albicans and C. tropicalis at 48 and 72 h of growth by scanning electron microscopy using different magnifications (1.67, 3.33, 16.7, and 66.7 kx). Time samples of 48 and 72 h were used to compare the biofilm structure as well as cell morphology and area of the biofilms using a Tescan Mira 3 scanning electron microscope equipped with a Schottky field emission gun (Schottky FEG-SEM). Yeast cell area was calculated from the best pictures by Fiji ImageJ version 1.57 (Schindelin et al., 2012). |
PMC9433541_f3_390455.jpg | Describe the main subject of this image. | Illustration of the biofilms of C. albicans and C. tropicalis at 48 and 72 h of growth by scanning electron microscopy using different magnifications (1.67, 3.33, 16.7, and 66.7 kx). Time samples of 48 and 72 h were used to compare the biofilm structure as well as cell morphology and area of the biofilms using a Tescan Mira 3 scanning electron microscope equipped with a Schottky field emission gun (Schottky FEG-SEM). Yeast cell area was calculated from the best pictures by Fiji ImageJ version 1.57 (Schindelin et al., 2012). |
PMC9433541_f3_390451.jpg | What can you see in this picture? | Illustration of the biofilms of C. albicans and C. tropicalis at 48 and 72 h of growth by scanning electron microscopy using different magnifications (1.67, 3.33, 16.7, and 66.7 kx). Time samples of 48 and 72 h were used to compare the biofilm structure as well as cell morphology and area of the biofilms using a Tescan Mira 3 scanning electron microscope equipped with a Schottky field emission gun (Schottky FEG-SEM). Yeast cell area was calculated from the best pictures by Fiji ImageJ version 1.57 (Schindelin et al., 2012). |
PMC9433541_f3_390460.jpg | What stands out most in this visual? | Illustration of the biofilms of C. albicans and C. tropicalis at 48 and 72 h of growth by scanning electron microscopy using different magnifications (1.67, 3.33, 16.7, and 66.7 kx). Time samples of 48 and 72 h were used to compare the biofilm structure as well as cell morphology and area of the biofilms using a Tescan Mira 3 scanning electron microscope equipped with a Schottky field emission gun (Schottky FEG-SEM). Yeast cell area was calculated from the best pictures by Fiji ImageJ version 1.57 (Schindelin et al., 2012). |
PMC9433541_f3_390448.jpg | What is the principal component of this image? | Illustration of the biofilms of C. albicans and C. tropicalis at 48 and 72 h of growth by scanning electron microscopy using different magnifications (1.67, 3.33, 16.7, and 66.7 kx). Time samples of 48 and 72 h were used to compare the biofilm structure as well as cell morphology and area of the biofilms using a Tescan Mira 3 scanning electron microscope equipped with a Schottky field emission gun (Schottky FEG-SEM). Yeast cell area was calculated from the best pictures by Fiji ImageJ version 1.57 (Schindelin et al., 2012). |
PMC9433559_f2_390461.jpg | What key item or scene is captured in this photo? | Histological features. Skin biopsy demonstrated mild exocytosis, basal layer vacuolization, lymphocyte infiltrate at the interface, and mild melanin incontinence (hematoxylin and eosin stain, A: 200×, B: 400×). |
PMC9433559_f2_390462.jpg | Describe the main subject of this image. | Histological features. Skin biopsy demonstrated mild exocytosis, basal layer vacuolization, lymphocyte infiltrate at the interface, and mild melanin incontinence (hematoxylin and eosin stain, A: 200×, B: 400×). |
PMC9433570_f4_390473.jpg | Can you identify the primary element in this image? | Effect of macrophage polarization on the radiographic changes and histological scores corresponding to rat coccygeal IVDs. (A) Representative general view, HE staining and Safranin-O/Fast Green staining images of rat coccygeal IVDs corresponding to different treatment groups (Coronal position). (B) Histological grading score showing changes in IVD at 8 weeks after initial puncture and different treatments. (C) Representative X-ray images showing rat coccygeal IVDs in different treatment groups and (D), %DHI values showing changes in IVD at 8 weeks after initial puncture for different treatments. All data are expressed as the mean ± SD. n = 6, *P < 0.05, **P < 0.01. IVD, intervertebral disc; TNF-α, tumor necrosis factor-α; CM, condition medium; HE, Hematoxylin and eosin; DHI, disc height index. |
PMC9433570_f4_390474.jpg | What is the main focus of this visual representation? | Effect of macrophage polarization on the radiographic changes and histological scores corresponding to rat coccygeal IVDs. (A) Representative general view, HE staining and Safranin-O/Fast Green staining images of rat coccygeal IVDs corresponding to different treatment groups (Coronal position). (B) Histological grading score showing changes in IVD at 8 weeks after initial puncture and different treatments. (C) Representative X-ray images showing rat coccygeal IVDs in different treatment groups and (D), %DHI values showing changes in IVD at 8 weeks after initial puncture for different treatments. All data are expressed as the mean ± SD. n = 6, *P < 0.05, **P < 0.01. IVD, intervertebral disc; TNF-α, tumor necrosis factor-α; CM, condition medium; HE, Hematoxylin and eosin; DHI, disc height index. |
PMC9433570_f4_390476.jpg | Can you identify the primary element in this image? | Effect of macrophage polarization on the radiographic changes and histological scores corresponding to rat coccygeal IVDs. (A) Representative general view, HE staining and Safranin-O/Fast Green staining images of rat coccygeal IVDs corresponding to different treatment groups (Coronal position). (B) Histological grading score showing changes in IVD at 8 weeks after initial puncture and different treatments. (C) Representative X-ray images showing rat coccygeal IVDs in different treatment groups and (D), %DHI values showing changes in IVD at 8 weeks after initial puncture for different treatments. All data are expressed as the mean ± SD. n = 6, *P < 0.05, **P < 0.01. IVD, intervertebral disc; TNF-α, tumor necrosis factor-α; CM, condition medium; HE, Hematoxylin and eosin; DHI, disc height index. |
PMC9433570_f4_390472.jpg | What key item or scene is captured in this photo? | Effect of macrophage polarization on the radiographic changes and histological scores corresponding to rat coccygeal IVDs. (A) Representative general view, HE staining and Safranin-O/Fast Green staining images of rat coccygeal IVDs corresponding to different treatment groups (Coronal position). (B) Histological grading score showing changes in IVD at 8 weeks after initial puncture and different treatments. (C) Representative X-ray images showing rat coccygeal IVDs in different treatment groups and (D), %DHI values showing changes in IVD at 8 weeks after initial puncture for different treatments. All data are expressed as the mean ± SD. n = 6, *P < 0.05, **P < 0.01. IVD, intervertebral disc; TNF-α, tumor necrosis factor-α; CM, condition medium; HE, Hematoxylin and eosin; DHI, disc height index. |
PMC9433570_f4_390485.jpg | What is the principal component of this image? | Effect of macrophage polarization on the radiographic changes and histological scores corresponding to rat coccygeal IVDs. (A) Representative general view, HE staining and Safranin-O/Fast Green staining images of rat coccygeal IVDs corresponding to different treatment groups (Coronal position). (B) Histological grading score showing changes in IVD at 8 weeks after initial puncture and different treatments. (C) Representative X-ray images showing rat coccygeal IVDs in different treatment groups and (D), %DHI values showing changes in IVD at 8 weeks after initial puncture for different treatments. All data are expressed as the mean ± SD. n = 6, *P < 0.05, **P < 0.01. IVD, intervertebral disc; TNF-α, tumor necrosis factor-α; CM, condition medium; HE, Hematoxylin and eosin; DHI, disc height index. |
PMC9433570_f4_390482.jpg | What is the main focus of this visual representation? | Effect of macrophage polarization on the radiographic changes and histological scores corresponding to rat coccygeal IVDs. (A) Representative general view, HE staining and Safranin-O/Fast Green staining images of rat coccygeal IVDs corresponding to different treatment groups (Coronal position). (B) Histological grading score showing changes in IVD at 8 weeks after initial puncture and different treatments. (C) Representative X-ray images showing rat coccygeal IVDs in different treatment groups and (D), %DHI values showing changes in IVD at 8 weeks after initial puncture for different treatments. All data are expressed as the mean ± SD. n = 6, *P < 0.05, **P < 0.01. IVD, intervertebral disc; TNF-α, tumor necrosis factor-α; CM, condition medium; HE, Hematoxylin and eosin; DHI, disc height index. |
PMC9433570_f4_390484.jpg | What stands out most in this visual? | Effect of macrophage polarization on the radiographic changes and histological scores corresponding to rat coccygeal IVDs. (A) Representative general view, HE staining and Safranin-O/Fast Green staining images of rat coccygeal IVDs corresponding to different treatment groups (Coronal position). (B) Histological grading score showing changes in IVD at 8 weeks after initial puncture and different treatments. (C) Representative X-ray images showing rat coccygeal IVDs in different treatment groups and (D), %DHI values showing changes in IVD at 8 weeks after initial puncture for different treatments. All data are expressed as the mean ± SD. n = 6, *P < 0.05, **P < 0.01. IVD, intervertebral disc; TNF-α, tumor necrosis factor-α; CM, condition medium; HE, Hematoxylin and eosin; DHI, disc height index. |
PMC9433570_f4_390480.jpg | What is the dominant medical problem in this image? | Effect of macrophage polarization on the radiographic changes and histological scores corresponding to rat coccygeal IVDs. (A) Representative general view, HE staining and Safranin-O/Fast Green staining images of rat coccygeal IVDs corresponding to different treatment groups (Coronal position). (B) Histological grading score showing changes in IVD at 8 weeks after initial puncture and different treatments. (C) Representative X-ray images showing rat coccygeal IVDs in different treatment groups and (D), %DHI values showing changes in IVD at 8 weeks after initial puncture for different treatments. All data are expressed as the mean ± SD. n = 6, *P < 0.05, **P < 0.01. IVD, intervertebral disc; TNF-α, tumor necrosis factor-α; CM, condition medium; HE, Hematoxylin and eosin; DHI, disc height index. |
PMC9433570_f4_390471.jpg | What is the principal component of this image? | Effect of macrophage polarization on the radiographic changes and histological scores corresponding to rat coccygeal IVDs. (A) Representative general view, HE staining and Safranin-O/Fast Green staining images of rat coccygeal IVDs corresponding to different treatment groups (Coronal position). (B) Histological grading score showing changes in IVD at 8 weeks after initial puncture and different treatments. (C) Representative X-ray images showing rat coccygeal IVDs in different treatment groups and (D), %DHI values showing changes in IVD at 8 weeks after initial puncture for different treatments. All data are expressed as the mean ± SD. n = 6, *P < 0.05, **P < 0.01. IVD, intervertebral disc; TNF-α, tumor necrosis factor-α; CM, condition medium; HE, Hematoxylin and eosin; DHI, disc height index. |
PMC9433570_f4_390478.jpg | What is the core subject represented in this visual? | Effect of macrophage polarization on the radiographic changes and histological scores corresponding to rat coccygeal IVDs. (A) Representative general view, HE staining and Safranin-O/Fast Green staining images of rat coccygeal IVDs corresponding to different treatment groups (Coronal position). (B) Histological grading score showing changes in IVD at 8 weeks after initial puncture and different treatments. (C) Representative X-ray images showing rat coccygeal IVDs in different treatment groups and (D), %DHI values showing changes in IVD at 8 weeks after initial puncture for different treatments. All data are expressed as the mean ± SD. n = 6, *P < 0.05, **P < 0.01. IVD, intervertebral disc; TNF-α, tumor necrosis factor-α; CM, condition medium; HE, Hematoxylin and eosin; DHI, disc height index. |
PMC9433570_f4_390463.jpg | What is shown in this image? | Effect of macrophage polarization on the radiographic changes and histological scores corresponding to rat coccygeal IVDs. (A) Representative general view, HE staining and Safranin-O/Fast Green staining images of rat coccygeal IVDs corresponding to different treatment groups (Coronal position). (B) Histological grading score showing changes in IVD at 8 weeks after initial puncture and different treatments. (C) Representative X-ray images showing rat coccygeal IVDs in different treatment groups and (D), %DHI values showing changes in IVD at 8 weeks after initial puncture for different treatments. All data are expressed as the mean ± SD. n = 6, *P < 0.05, **P < 0.01. IVD, intervertebral disc; TNF-α, tumor necrosis factor-α; CM, condition medium; HE, Hematoxylin and eosin; DHI, disc height index. |
PMC9433570_f4_390468.jpg | What is the central feature of this picture? | Effect of macrophage polarization on the radiographic changes and histological scores corresponding to rat coccygeal IVDs. (A) Representative general view, HE staining and Safranin-O/Fast Green staining images of rat coccygeal IVDs corresponding to different treatment groups (Coronal position). (B) Histological grading score showing changes in IVD at 8 weeks after initial puncture and different treatments. (C) Representative X-ray images showing rat coccygeal IVDs in different treatment groups and (D), %DHI values showing changes in IVD at 8 weeks after initial puncture for different treatments. All data are expressed as the mean ± SD. n = 6, *P < 0.05, **P < 0.01. IVD, intervertebral disc; TNF-α, tumor necrosis factor-α; CM, condition medium; HE, Hematoxylin and eosin; DHI, disc height index. |
PMC9433570_f4_390483.jpg | What is being portrayed in this visual content? | Effect of macrophage polarization on the radiographic changes and histological scores corresponding to rat coccygeal IVDs. (A) Representative general view, HE staining and Safranin-O/Fast Green staining images of rat coccygeal IVDs corresponding to different treatment groups (Coronal position). (B) Histological grading score showing changes in IVD at 8 weeks after initial puncture and different treatments. (C) Representative X-ray images showing rat coccygeal IVDs in different treatment groups and (D), %DHI values showing changes in IVD at 8 weeks after initial puncture for different treatments. All data are expressed as the mean ± SD. n = 6, *P < 0.05, **P < 0.01. IVD, intervertebral disc; TNF-α, tumor necrosis factor-α; CM, condition medium; HE, Hematoxylin and eosin; DHI, disc height index. |
PMC9433570_f4_390477.jpg | What key item or scene is captured in this photo? | Effect of macrophage polarization on the radiographic changes and histological scores corresponding to rat coccygeal IVDs. (A) Representative general view, HE staining and Safranin-O/Fast Green staining images of rat coccygeal IVDs corresponding to different treatment groups (Coronal position). (B) Histological grading score showing changes in IVD at 8 weeks after initial puncture and different treatments. (C) Representative X-ray images showing rat coccygeal IVDs in different treatment groups and (D), %DHI values showing changes in IVD at 8 weeks after initial puncture for different treatments. All data are expressed as the mean ± SD. n = 6, *P < 0.05, **P < 0.01. IVD, intervertebral disc; TNF-α, tumor necrosis factor-α; CM, condition medium; HE, Hematoxylin and eosin; DHI, disc height index. |
PMC9433570_f4_390479.jpg | What is the central feature of this picture? | Effect of macrophage polarization on the radiographic changes and histological scores corresponding to rat coccygeal IVDs. (A) Representative general view, HE staining and Safranin-O/Fast Green staining images of rat coccygeal IVDs corresponding to different treatment groups (Coronal position). (B) Histological grading score showing changes in IVD at 8 weeks after initial puncture and different treatments. (C) Representative X-ray images showing rat coccygeal IVDs in different treatment groups and (D), %DHI values showing changes in IVD at 8 weeks after initial puncture for different treatments. All data are expressed as the mean ± SD. n = 6, *P < 0.05, **P < 0.01. IVD, intervertebral disc; TNF-α, tumor necrosis factor-α; CM, condition medium; HE, Hematoxylin and eosin; DHI, disc height index. |
PMC9433570_f4_390466.jpg | What is the main focus of this visual representation? | Effect of macrophage polarization on the radiographic changes and histological scores corresponding to rat coccygeal IVDs. (A) Representative general view, HE staining and Safranin-O/Fast Green staining images of rat coccygeal IVDs corresponding to different treatment groups (Coronal position). (B) Histological grading score showing changes in IVD at 8 weeks after initial puncture and different treatments. (C) Representative X-ray images showing rat coccygeal IVDs in different treatment groups and (D), %DHI values showing changes in IVD at 8 weeks after initial puncture for different treatments. All data are expressed as the mean ± SD. n = 6, *P < 0.05, **P < 0.01. IVD, intervertebral disc; TNF-α, tumor necrosis factor-α; CM, condition medium; HE, Hematoxylin and eosin; DHI, disc height index. |
PMC9433570_f4_390469.jpg | What is the principal component of this image? | Effect of macrophage polarization on the radiographic changes and histological scores corresponding to rat coccygeal IVDs. (A) Representative general view, HE staining and Safranin-O/Fast Green staining images of rat coccygeal IVDs corresponding to different treatment groups (Coronal position). (B) Histological grading score showing changes in IVD at 8 weeks after initial puncture and different treatments. (C) Representative X-ray images showing rat coccygeal IVDs in different treatment groups and (D), %DHI values showing changes in IVD at 8 weeks after initial puncture for different treatments. All data are expressed as the mean ± SD. n = 6, *P < 0.05, **P < 0.01. IVD, intervertebral disc; TNF-α, tumor necrosis factor-α; CM, condition medium; HE, Hematoxylin and eosin; DHI, disc height index. |
PMC9433570_f4_390481.jpg | What is the main focus of this visual representation? | Effect of macrophage polarization on the radiographic changes and histological scores corresponding to rat coccygeal IVDs. (A) Representative general view, HE staining and Safranin-O/Fast Green staining images of rat coccygeal IVDs corresponding to different treatment groups (Coronal position). (B) Histological grading score showing changes in IVD at 8 weeks after initial puncture and different treatments. (C) Representative X-ray images showing rat coccygeal IVDs in different treatment groups and (D), %DHI values showing changes in IVD at 8 weeks after initial puncture for different treatments. All data are expressed as the mean ± SD. n = 6, *P < 0.05, **P < 0.01. IVD, intervertebral disc; TNF-α, tumor necrosis factor-α; CM, condition medium; HE, Hematoxylin and eosin; DHI, disc height index. |
PMC9433574_f2_390492.jpg | What is being portrayed in this visual content? |
(A) Immunoreactivity for cytokeratin 7. (B) Negativity for cytokeratin 20. (C, E) HER2 strong membranous staining (3+) at immunohistochemical stain magnification, × 10, in two different tumor samples of the patient. (D, F) microscopic image in CISH for HER2 staining in two different tumor samples of the patient. (G) Androgen receptor staining at immunohistochemical stain magnification, × 10. |
PMC9433574_f2_390487.jpg | Describe the main subject of this image. |
(A) Immunoreactivity for cytokeratin 7. (B) Negativity for cytokeratin 20. (C, E) HER2 strong membranous staining (3+) at immunohistochemical stain magnification, × 10, in two different tumor samples of the patient. (D, F) microscopic image in CISH for HER2 staining in two different tumor samples of the patient. (G) Androgen receptor staining at immunohistochemical stain magnification, × 10. |
PMC9433574_f2_390490.jpg | What is the dominant medical problem in this image? |
(A) Immunoreactivity for cytokeratin 7. (B) Negativity for cytokeratin 20. (C, E) HER2 strong membranous staining (3+) at immunohistochemical stain magnification, × 10, in two different tumor samples of the patient. (D, F) microscopic image in CISH for HER2 staining in two different tumor samples of the patient. (G) Androgen receptor staining at immunohistochemical stain magnification, × 10. |
PMC9433574_f3_390494.jpg | What key item or scene is captured in this photo? | FDG PET/CT scan at baseline of patient’s clinical history. |
PMC9433574_f3_390493.jpg | What is being portrayed in this visual content? | FDG PET/CT scan at baseline of patient’s clinical history. |
PMC9433645_f7_390501.jpg | Describe the main subject of this image. |
C5b-9 immunofluorescent staining showed C5b-9 deposition (red) (A) on the blood vessels in lung tissue in 5/7 of the untreated animals and in none of the four treated animals. (B) In kidney tissue, C5b-9 glomerular deposition was detected in 5/7 untreated and in 2/4 treated animals. (C) In the liver tissue, C5b-9 deposition was detected in 5/7 untreated and in none if the treated animals. (D) C5b-9 deposition was also detected in the liver blood vessels in the untreated but not in the treated group. One representative section from each group is shown. To facilitate the recognition of the histological structures the nuclear counterstaining (blue) and F-actin counterstaining (green) have been performed. In the upper panel, in the right upper corner, the respective ‘no primary antibody controls’ are illustrated. Magnification bars (short white lines bottom right in all panels): 20µm (lung) and 50µm (kidney and liver). |
PMC9433645_f7_390497.jpg | What's the most prominent thing you notice in this picture? |
C5b-9 immunofluorescent staining showed C5b-9 deposition (red) (A) on the blood vessels in lung tissue in 5/7 of the untreated animals and in none of the four treated animals. (B) In kidney tissue, C5b-9 glomerular deposition was detected in 5/7 untreated and in 2/4 treated animals. (C) In the liver tissue, C5b-9 deposition was detected in 5/7 untreated and in none if the treated animals. (D) C5b-9 deposition was also detected in the liver blood vessels in the untreated but not in the treated group. One representative section from each group is shown. To facilitate the recognition of the histological structures the nuclear counterstaining (blue) and F-actin counterstaining (green) have been performed. In the upper panel, in the right upper corner, the respective ‘no primary antibody controls’ are illustrated. Magnification bars (short white lines bottom right in all panels): 20µm (lung) and 50µm (kidney and liver). |
PMC9433645_f7_390502.jpg | What is the core subject represented in this visual? |
C5b-9 immunofluorescent staining showed C5b-9 deposition (red) (A) on the blood vessels in lung tissue in 5/7 of the untreated animals and in none of the four treated animals. (B) In kidney tissue, C5b-9 glomerular deposition was detected in 5/7 untreated and in 2/4 treated animals. (C) In the liver tissue, C5b-9 deposition was detected in 5/7 untreated and in none if the treated animals. (D) C5b-9 deposition was also detected in the liver blood vessels in the untreated but not in the treated group. One representative section from each group is shown. To facilitate the recognition of the histological structures the nuclear counterstaining (blue) and F-actin counterstaining (green) have been performed. In the upper panel, in the right upper corner, the respective ‘no primary antibody controls’ are illustrated. Magnification bars (short white lines bottom right in all panels): 20µm (lung) and 50µm (kidney and liver). |
PMC9433645_f7_390499.jpg | What stands out most in this visual? |
C5b-9 immunofluorescent staining showed C5b-9 deposition (red) (A) on the blood vessels in lung tissue in 5/7 of the untreated animals and in none of the four treated animals. (B) In kidney tissue, C5b-9 glomerular deposition was detected in 5/7 untreated and in 2/4 treated animals. (C) In the liver tissue, C5b-9 deposition was detected in 5/7 untreated and in none if the treated animals. (D) C5b-9 deposition was also detected in the liver blood vessels in the untreated but not in the treated group. One representative section from each group is shown. To facilitate the recognition of the histological structures the nuclear counterstaining (blue) and F-actin counterstaining (green) have been performed. In the upper panel, in the right upper corner, the respective ‘no primary antibody controls’ are illustrated. Magnification bars (short white lines bottom right in all panels): 20µm (lung) and 50µm (kidney and liver). |
PMC9433646_f2_390503.jpg | Describe the main subject of this image. | ASL-MRI on 9 February 2021 before GV-971 treatment (A), 29 July 2021 (B), and 18 January 2022 (C). After treatment with GV-971 (B, C), regions of low perfusion in green were visibly decreased. |
PMC9433646_f2_390505.jpg | What is the principal component of this image? | ASL-MRI on 9 February 2021 before GV-971 treatment (A), 29 July 2021 (B), and 18 January 2022 (C). After treatment with GV-971 (B, C), regions of low perfusion in green were visibly decreased. |
PMC9433646_f2_390504.jpg | What object or scene is depicted here? | ASL-MRI on 9 February 2021 before GV-971 treatment (A), 29 July 2021 (B), and 18 January 2022 (C). After treatment with GV-971 (B, C), regions of low perfusion in green were visibly decreased. |
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