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How dose it define the peak？
Thank you very much for providing such a good app to identify foci.
Could you tell me how dose it define the peak?And can I use it to identify dense focis like RPA（pachytene）or HEI10（in early pachytene）?And how?
If you have a detailed introduction could tell me where can I find it？
Thank you!
Hi - the app implements the methods in https://www.nature.com/articles/s41467-021-24827-w ; so it traces along each path and uses a peak-finding algorithm (from scipy) on these one-dimensional peaks.
There's some more details in https://pubmed.ncbi.nlm.nih.gov/39126479/ ; if you send me a DM I might be able to find a reprint of it
For dense foci, like early HEI10, you'd need to first find the peaks and then position them on the chromosomes, like we did in https://pubmed.ncbi.nlm.nih.gov/36847348/
There's code at https://github.com/jfozard/hei10_zyp1/tree/main/image_analysis , but not in the form of an app. If that's something that could be of use to you then let me know.
John