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Toll-like receptors as key mediators in innate antifungal immunity. | The Toll protein of Drosophila is a transmembrane receptor involved in dorsoventral polarization during embryonic development and recognition of infection. In mammals; Toll-like receptors (TLRs) constitute a novel protein family involved in innate immunity and respond to a wide spectrum of microorganisms; including fungi; bacteria; viruses; and protozoa. Specific agonists for nine of the ten members of the human TLR family have been described to date. TLRs as well as the TLR-associated adaptor molecule MyD8 | 15682636 | [
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Toll-like receptors as key mediators in innate antifungal immunity. | 8 have been implicated in the recognition of the fungal pathogens Candida albicans; Aspergillus fumigatus; Cryptococcus neoformans and Pneumocystis carinii. Moreover; several pathogen associated molecular patterns (PAMPs) located in the cell wall or cell surface of fungi have been identified as potential ligands. Yeast zymosan activates TLR2/ TLR6 heterodimers; whereas Saccharomyces cerevisiae- and C. albicans-derived mannan seems to be detected by TLR4. Phospholipomannan; present in the cell surface of C. | 15682636 | [
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Toll-like receptors as key mediators in innate antifungal immunity. | albicans has been shown to be recognized by TLR2; while TLR4 mainly interacts with glucuronoxylomannan; the major capsular polysaccharide of C. neoformans. MyD88 has been implicated in TLR signalling of linear (1 --> 3)-beta-D-glucan; and of beta-glucan from P. carinii. These data point towards the ability of the innate immune system to utilize TLRs that are specific to different types and components of pathogenic fungi. Recent evidence further suggests that TLRs cooperate with other immune receptors involv | 15682636 | [
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Toll-like receptors as key mediators in innate antifungal immunity. | ed in fungal recognition and that the selective induction of adaptor proteins finally leads to distinct signalling events upon fungal challenge. | 15682636 | [
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Evaluation of Microsporum canis in different methods of storage. | The main objective of this investigation was to evaluate different methods of storage for Microsporum canis based on materials and equipment that are readily available in developing countries. We tested 32 strains of M. canis at - 20 degrees C in potato dextrose agar (PDA) in its plain condition; or amended with 10% dimethyl sulfoxide or with 10% glycerol. In addition; we tested 25 degrees C storage of isolates in plain saline (0.9% NaCl) and in saline covered with a mineral-oil layer. After 9 months of sto | 15682637 | [
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Evaluation of Microsporum canis in different methods of storage. | rage; none of the M. canis strains frozen in PDA supplemented with glycerol survived; while only 16 and 6%; respectively; of the isolates in plain and DMSO medium lost viability. Nine month's storage in saline with or without mineral oil increased the amount of pleomorphic development of sterile hyphae; this phenomenon occurred at a significantly higher level than was seen in isolates stored at -20 degrees C. The physiological characteristics of M. canis were not affected by the different storage tests. The | 15682637 | [
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Evaluation of Microsporum canis in different methods of storage. | results suggest that; in order to ensure optimal viability; purity and pristine isolate condition; each M. canis isolate maintained should be held in at least two methods of storage; namely; PDA at -20 degrees C and saline with a mineral-oil layer at 25 degrees C. | 15682637 | [
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Increasing tinea capitis prevalence in Stockholm reflects immigration. | The aim was to describe the mycological and clinical data in children diagnosed with tinea capitis in a hospital setting in Stockholm. Information concerning demography; symptoms; mycology and treatment were obtained; retrospectively; from medical records of all children up to 15 years of age diagnosed with tinea capitis during two 3-year periods; 1989--1991 and 1999--2001; at the Pediatric Dermatology Unit of the Karolinska Hospital in Stockholm. Between 1989 and 1991; five children were diagnosed with tin | 15682638 | [
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Increasing tinea capitis prevalence in Stockholm reflects immigration. | ea capitis. Between 1999 and 2001; there were 92 children; the vast majority (86%) being of foreign extraction; mostly African (83%). Trichophyton violaceum was the most prevalent pathogen; affecting 68% of the children. Of the anthropophilic infections; 62% were linked to relatives. In 71% of all positive cultures; microscopy was positive. The most common clinical findings were scaling of the scalp (80%); itching (54%) and patches of alopecia (52%). The treatment consisted of the oral antimycotics terbinaf | 15682638 | [
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Increasing tinea capitis prevalence in Stockholm reflects immigration. | ine (n = 48) or griseofulvin (n = 49). During the last decade there has been an increase in tinea capitis in Stockholm; most commonly caused by Trichophyton violaceum; corresponding with the increased immigration from Africa. Spread within the family seems to be of importance; and family members are preferably screened in an effort to prevent continued transmission. It is important to bear the diagnosis of tinea capitis in mind; especially as; untreated; some cases can develop permanent alopecia and may als | 15682638 | [
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Highly specific and sensitive, immunoblot-detected 54 kDa antigen from Fonsecaea pedrosoi. | Chromoblastomycosis (CBM) is a chronic subcutaneous mycosis caused by a group of different dematiaceous fungi; first described by Rudolph in 1914. In Brazil there is a clear predominance of Fonsecaea pedrosoi. Sixty sera samples obtained from patients with F. pedrosoi-caused CBM were analysed. Sera obtained from 36 sporothricosis (SPT) patients; 34 cutaneous leishmaniasis (CL) patients and from 48 blood donors (HBD) were used as control. F. pedrosoi metabolic antigen was obtained from F. pedrosoi sample no. | 15682639 | [
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Highly specific and sensitive, immunoblot-detected 54 kDa antigen from Fonsecaea pedrosoi. | 884 (Instituto de Medicina Tropical de São Paulo Collection). IE reaction disclosed an anodic migrating arch; which was eluted and used as antigen. Both metabolic and eluate F. pedrosoi antigens were submitted to SDS-PAGE and two fractions; weighing approximately 54 and 66 kDa were identified. The 66-kDa fraction reacted against 43 of 60 CBM (71.7%) sera samples and was recognized by 10 SPT and eight CL sera (15.3%). No reactivity was observed against HBD sera. The 54-kDa fraction reacted against 58 of 60 | 15682639 | [
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Highly specific and sensitive, immunoblot-detected 54 kDa antigen from Fonsecaea pedrosoi. | CBM sera (96.7% sensitivity) and was not recognized by HBD; SPT nor CL sera (100% specificity). Such high sensitivity and specificity levels suggest this antigenic fraction is immunodominant and might prove a useful tool for further studies on F. pedrosoi-caused CBM. | 15682639 | [
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Distribution of paracoccidioidomycosis: determination of ecologic correlates through spatial analyses. | Paracoccidioidomycosis (PCM) is endemic in Latin America and in countries like Brazil it carries a high mortality rate. The fungus' habitat has not been precisely determined. The present study aims to identify ecologic correlates based on PCM distribution in a hyper-endemic area in southeastern Brazil. The Geographic Information System (GIS) and spatial statistics were used to associate environmental attributes; human population density and; PCM distribution. By means of the Pearson r correlation coefficien | 15682640 | [
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Distribution of paracoccidioidomycosis: determination of ecologic correlates through spatial analyses. | t; the highest statistically significant associations with prevalence density were the percent area (by county) of: basaltic rocks (r=0.63; P <0.0001); Podzolic soils (r= -0.48; P <0.001); Latosol soils (r=0.40; P <0.01); mean annual precipitation between 1500 and 1600 mm (r = 0.46; P < 0.001) and; mean precipitation during the wet season between 940 and 1040 mm (r= -0.44; P <0.01). Soil texture and precipitation analyzed together reached r=0.61 (P <0.000002) for fine-textured soils with annual precipitatio | 15682640 | [
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Distribution of paracoccidioidomycosis: determination of ecologic correlates through spatial analyses. | n above 1400 mm. Environmental correlates indicate that moisture availability plays an important role in PCM distribution. | 15682640 | [
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Biochemical characterization of terbinafine-resistant Trichophyton rubrum isolates. | We investigated the biochemical basis for resistance in six sequential clinical isolates of Trichophyton rubrum; from the same patient; which exhibited high-level primary resistance to terbinafine. Cellular ergosterol biosynthesis was measured by incorporation of [14C]acetate; and microsomal squalene epoxidase was assayed by conversion of [3H]squalene to squalene epoxide and lanosterol. Direct comparison was made with a terbinafine-susceptible reference strain of T. rubrum in which squalene epoxidase was pr | 15682641 | [
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Biochemical characterization of terbinafine-resistant Trichophyton rubrum isolates. | eviously studied. Resistant isolates displayed normal cellular ergosterol biosynthesis; although slight accumulation of radiolabeled squalene suggested reduced squalene epoxidase activity. Ergosterol biosynthesis in the resistant isolates was only inhibited by terbinafine concentrations above 1 microg/ml (IC50 5 microg/ml). In the reference strain; ergosterol biosynthesis was eliminated by terbinafine at 0.03 microg/ml in accordance with historical data. There was no significant difference in sensitivity be | 15682641 | [
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Biochemical characterization of terbinafine-resistant Trichophyton rubrum isolates. | tween the six resistant isolates. Squalene epoxidase from resistant strains was three orders of magnitude less sensitive than normal enzyme to terbinafine (IC50 of 30 micromol/l and 19 n mol/l respectively). The epoxidase in the resistant strains was also unresponsive to tolnaftate. Resistance to terbinafine in these T. rubrum isolates appears to be due to alterations in the squalene epoxidase gene or a factor essential for its activity. | 15682641 | [
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Bioactive interleukin-1alpha is cytolytically released from Candida albicans-infected oral epithelial cells. | Oral epithelial cells are primary targets of Candida albicans in the oropharynx and may regulate the inflammatory host response to this pathogen. This investigation studied the mechanisms underlying interleukin-1alpha (IL-1alpha) release by oral epithelial cells and the role of IL-1alpha in regulating the mucosal inflammatory response to C. albicans. Infected oral epithelial cells released processed IL-1alpha protein in culture supernatants. The IL-1alpha generated was stored intracellularly and was release | 15682642 | [
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Bioactive interleukin-1alpha is cytolytically released from Candida albicans-infected oral epithelial cells. | d upon cell lysis. This was further supported by the fact that different C. albicans strains induced variable IL-1alpha release; depending on their cytolytic activity. IL-1alpha from C. albicans-infected oral epithelial cells upregulated proinflammatory cytokine secretion (IL-8 and GM-CSF) in uninfected oral epithelial or stromal cells. Our studies suggest that production of IL-1alpha; IL-8 and GM-CSF may take place in the oral mucosa in response to lytic infection of epithelial cells with C. albicans. This | 15682642 | [
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Bioactive interleukin-1alpha is cytolytically released from Candida albicans-infected oral epithelial cells. | process can act as an early innate immune surveillance system and may contribute to the clinicopathologic signs of infection in the oral mucosa. | 15682642 | [
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Subcutaneous phaeohyphomycosis caused by Lasiodiplodia theobromae and successfully treated surgically. | While visiting Jamaica; a 50-year-old woman stumbled on an outdoor wooden staircase and sustained an injury to the right leg. The wound was cleaned topically and the patient was given antibacterial therapy. Five weeks later; in Canada; she presented with an ulcer at the injury site. An excisional biopsy showed copious broad; septate; melanized fungal filaments penetrating into tissue. Culture yielded a nonsporulating melanized mycelium. The isolate was strongly inhibited by cycloheximide and benomyl but gre | 15682643 | [
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Subcutaneous phaeohyphomycosis caused by Lasiodiplodia theobromae and successfully treated surgically. | w at 37 degrees C. After 16 weeks cultivation on modified Leonian's agar at 25 degrees C; it developed pycnidia characteristic of Lasiodiplodia theobromae; a common tropical phytopathogen mainly known previously as a rare agent of keratitis and onychomycosis in humans. The patient was not given antifungal chemotherapy; and the ulcer; which had been broadly excised in the biopsy procedure; ultimately resolved after treatment with saline compresses. The six-month follow-up showed no sign of infection. This ca | 15682643 | [
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Subcutaneous phaeohyphomycosis caused by Lasiodiplodia theobromae and successfully treated surgically. | se; interpreted in light of previously reported cases; shows that on rare occasions L. theobromae is able to act as an agent of subcutaneous phaeohyphomycosis and that; when this occurs; debridement alone may be sufficient to eradicate it. | 15682643 | [
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Immune response in dogs experimentally infected with Paracoccidioides brasiliensis. | The aim of the present study was to evaluate the immune response of young dogs experimentally infected with Paracoccidioides brasiliensis. Six dogs were infected intravenously with P. brasiliensis and one control dog was inoculated with sterile saline. The infected animals were sacrificed in groups of two at 1; 6 and 12 months after infection. During the experimental period; the immune responses of the dogs to the fungus were followed by ELISA (IgM and IgG); by the immunodiffusion test and by the skin test | 15682644 | [
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Immune response in dogs experimentally infected with Paracoccidioides brasiliensis. | with gp43. After killing the dogs; samples from several organs were submitted to histopathological analysis (H&E and Grocott stains) but the fungus was not observed in any tissue. Attempts to isolate the fungus from these tissue samples were also unsuccessful. All infected dogs; except one; reacted positively to the immunodiffusion and skin tests. All infected dogs showed a humoral immune response to the gp43 antigen detected by ELISA. The IgM and IgG response peaked by the first and second month; respectiv | 15682644 | [
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Immune response in dogs experimentally infected with Paracoccidioides brasiliensis. | ely. We conclude that young dogs appear to be resistant to the development of paracoccidioidomycosis. | 15682644 | [
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Adiponectin: novelties in metabolism and hormonal regulation. | Adiponectin is a protein secreted exclusively by white adipose tissues and is abundantly present in human plasma. Adiponectin was found decreased in obese and diabetes mellitus type 2 patients and increased with weight reduction. A negative correlation between circulating adiponectin levels and body mass index and insulin resistance has been demonstrated. Plasma adiponectin concentrations were found lower in diabetes mellitus type 2 patients with coronary artery disease. Moreover; studies in aortic endothel | 15682645 | [
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Adiponectin: novelties in metabolism and hormonal regulation. | ial cells revealed that the protein exerts a dose-dependent decrease of the surface expression of vascular adhesion molecules and cytokine production from macrophages; suggesting the implication of adiponectin in atherosclerosis and inflammation. Weight loss and treatment with thiazolidinediones stimulate endogenous adiponectin production. Peripheral administration of adiponectin leads to reduction of visceral adiposity and increase of free fatty acid oxidation and insulin resistance. Furthermore; it enhanc | 15682645 | [
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Adiponectin: novelties in metabolism and hormonal regulation. | es the expression of uncoupling proteins and sympathetic nerve activity in adipose tissues. Experimental studies in mice have shown that intraperitoneal administration of adiponectin lowers plasma glucose. These data show adiponectin to be an important factor in the issue of obesity and its associated disorders; and indicate a potential future utilization of adiponectin as a drug in the treatment of metabolic syndrome. | 15682645 | [
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A low protein diet causes an increase in the basal levels and variability of mean arterial pressure and heart rate in Fisher rats. | The correlation between nutrition and cardiovascular related disorders is a well-established fact. Previous work from our Laboratory has suggested a significant compromise of cardiovascular reflexes in conscious rats submitted to a low-protein (LP) diet. Our working hypothesis is that the basal level of mean arterial pressure (MAP); variability of the mean arterial pressure (VMAP); heart rate (HR) and variability of heart rate (VHR) are altered in rats submitted to a protein restricted diet. Two experimenta | 15682646 | [
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A low protein diet causes an increase in the basal levels and variability of mean arterial pressure and heart rate in Fisher rats. | l groups were used: control group (normal protein 15%; NP) and malnourished group (low-protein 6%; LP). In order to verify the efficiency of the dietary restriction we measured body weight; total blood protein; plasma albumin; urea and glucose. Our experiments demonstrated that the malnourished rats presented augment levels of basal MAP (LP 122+/-2 mmHg vs. NP 113+/-1 mmHg) and of VMAP (LP 12.8+/-1.5mmHg vs. NP 9+/-1mmHg) when compared to the control group. We observed similar increased levels; in the malno | 15682646 | [
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A low protein diet causes an increase in the basal levels and variability of mean arterial pressure and heart rate in Fisher rats. | urished group; for both HR (LP 429+/-8 bpm vs. NP 381+/-7bpm) and VHR (LP 67.6+/-8.3bpm vs. NP 44.4+/-4.9bpm). Our results suggest a correlation between the LP diet in Fisher rats and the increased basal levels of mean arterial pressure; HR and their respective variability. | 15682646 | [
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NADPH-diaphorase histochemical labeling patterns in the hippocampal neuropil and visual cortical neurons in weaned rats reared during lactation on different litter sizes. | Tissue distribution of nitric oxide-synthases was investigated in the rat hippocampus and visual cortex under nutritional changes induced by modification of the litter size. Young (30-45-days-old) rats; suckled in litters formed by 3;6 or 12 pups (called small; medium and large litters; respectively); were studied by using nicotine-adenine-dinucleotide phosphate-diaphorase histochemistry (shortly; diaphorase); a simple and robust procedure to characterize tissue distribution of nitric oxide-synthases. We as | 15682647 | [
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NADPH-diaphorase histochemical labeling patterns in the hippocampal neuropil and visual cortical neurons in weaned rats reared during lactation on different litter sizes. | sessed morphometric features of the diaphorase-positive cells in visual cortex; and the neuropil histochemical activity in hippocampal CA1 and dentate gyrus using densitometry analysis. In the large-litter group; the labeled-cell density in white matter of area 17 was higher; as compared to the small-litter group. There was a clear trend; in the large-litter group; to lower values of soma area; dendritic field and branches per neuron; but the differences were not significant. Densitometry analysis of hippoc | 15682647 | [
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NADPH-diaphorase histochemical labeling patterns in the hippocampal neuropil and visual cortical neurons in weaned rats reared during lactation on different litter sizes. | ampus revealed a significant increase in the relative neuropil histochemical activity of the dentate gyrus molecular layer in the larger litters; which may be associated to increased compensatory blood flow in the hippocampus. The pathophysiological mechanisms of the observed changes remain to be investigated. | 15682647 | [
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Decreased UCP2 mRNA expression in rat stomach following vagotomy: novel role for UCP2 as free radical scavenger in the stomach? | Uncoupling protein 2 (UCP2) is a protein; located in the inner mitochondrial membrane; which dissipates the proton gradient of this membrane and uncouples respiration from oxidative phosphorylation. We found; by in situ hybridisation; UCP2 mRNA to be located in the proliferating zone of the mucous neck cells in the fundus part of the rat stomach. We also found that UCP2 expression in fundus was significantly decreased after seven days of vagotomy. Furthermore; we found manganese-containing superoxide dismut | 15682648 | [
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Decreased UCP2 mRNA expression in rat stomach following vagotomy: novel role for UCP2 as free radical scavenger in the stomach? | ase (SOD2); in fundus; to be down-regulated in a way similar to UCP2. The amount of ATP was significantly decreased following vagotomy. It is concluded that UCP2 in the gastro-intestinal tract is regulated through vagal innervation and suggested to act as a free radical scavenger. | 15682648 | [
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GFAP expression in astrocytes of suprachiasmatic nucleus and medial preoptic area are differentially affected by malnutrition during rat brain development. | The aim of the present study was investigate; in young rats; the effects of malnutrition on astrocyte distribution of two hypothalamic regions; the circadian pacemaker suprachiasmatic nucleus (SCN) and the medial preoptic area (MPA). Control rats were born from mothers fed on commercial diet since gestation and malnourished rats from mothers fed on multideficient diet; from the beginning of gestation (GLA group) or from the onset of lactation (LA group). After weaning; pups received ad libitum the same diet | 15682649 | [
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GFAP expression in astrocytes of suprachiasmatic nucleus and medial preoptic area are differentially affected by malnutrition during rat brain development. | as their mothers; and were maintained under a 12/12 h light/dark cycle. The animals were analyzed either at 30-33; or 60-63 days of life. Brain coronal sections (50 microm) were processed to visualize glial fibrillary acidic protein (GFAP) immunoreactivity. Compared to control rats; both malnourished groups of 30 and 60 days exhibited a reduced number of GFAP-immunoreactive astrocytes in the SCN. The total GFAP-immunoreactive area in the SCN of the GLA group differed from the control group at both age rang | 15682649 | [
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GFAP expression in astrocytes of suprachiasmatic nucleus and medial preoptic area are differentially affected by malnutrition during rat brain development. | es analyzed. The GFAP expression as measured by the relative optical density (ROD) exhibited a 50-60% reduction in the MPA in both malnourished groups; compared to controls. The results suggest that malnutrition early in life leads to alterations in gliogenesis or glial cell proliferation in both nuclei; being these alterations greater in the MPA. Compensatory plasticity mechanisms in the GFAP-expression seem to be developed in the astrocyte differentiation process in the SCN; especially when the malnutriti | 15682649 | [
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GFAP expression in astrocytes of suprachiasmatic nucleus and medial preoptic area are differentially affected by malnutrition during rat brain development. | on is installed from the lactation. | 15682649 | [
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Feeding induced by increasing doses of neuropeptide Y: dual effect on hypothalamic serotonin release in normal rats. | Endogenous neuropeptide Y (NPY) levels increase during fasting and before dark onset in rats. The feeding that follows these states elicits the release of serotonin in the lateral hypothalamus (LH); as part of the physiological mechanisms controlling satiety. With the hypothesis that exogenous NPY-induced feeding should also stimulate serotonin; we measured its release in the LH of non-fasted rats; which received a single intracerebroventricular injection of either 1.0; 2.0; or 5.0 microg of NPY. After 1.0 | 15682650 | [
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Feeding induced by increasing doses of neuropeptide Y: dual effect on hypothalamic serotonin release in normal rats. | microg; the cumulative 2-h intake was of 13 g and serotonin release significantly increased (54% peak). These feeding and serotonergic responses were highly similar to the ones we observed in a previous study; in which feeding followed an overnight fast. Thus; the 1.0 microg NPY dose stimulated intake while preserving the normal serotonergic activation. Contrarily; as the NPY dose was increased to either 2.0 or 5.0 microg; the cumulative 2-h intakes were of 18 g; but the serotonergic stimulation was absent. | 15682650 | [
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Feeding induced by increasing doses of neuropeptide Y: dual effect on hypothalamic serotonin release in normal rats. | It is suggested that this dual NPY effect relies on a finely tuned control mechanism; reflecting the existence of a narrow range of NPY levels within which the serotonergic stimulation resembles those seen in physiological states. | 15682650 | [
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Up-regulation of neuropeptide Y receptors in the hypothalamus of monosodium glutamate-lesioned Sprague-Dawley rats. | Monosodium-glutamate (MSG) is neurotoxic for brain regions devoid of blood-brain barrier when it is injected at high doses during the neonatal period. Neuropeptide Y (NPY) neurons in the arcuate nucleus are particularly sensitive to MSG treatment. But; despite of the large decrease of this potent orexigenic peptide; feeding behavior is only slightly affected. We hypothesized that the hypothalamic NPY receptor system might be modified in these rats. The present study characterizes hypothalamic NPY and NPY re | 15682651 | [
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Up-regulation of neuropeptide Y receptors in the hypothalamus of monosodium glutamate-lesioned Sprague-Dawley rats. | ceptors in normal and MSG-treated rats. MSG-treated rats were lighter (p < 0.01) and ate 17% less than the control rats (p < 0.01). NPY levels in the mediobasal and mediodorsal hypothalamus were reduced in MSG-treated rats compared to normal rats (-26% and -43%; p < 0.05 and p < 0.01; respectively). Combined hypothalamic Y1 and Y5 NPY receptor density was increased in MSG-treated rats compared to normal rats (+25%; p < 0.04); but affinity remained unaltered. Blockade with a selective Y1 antagonist showed th | 15682651 | [
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Up-regulation of neuropeptide Y receptors in the hypothalamus of monosodium glutamate-lesioned Sprague-Dawley rats. | at the Y1 receptor subtype represented more than 90% of the combined Y1 and Y5 receptor populations. The up-regulation of the NPY receptors is an element necessary to maintain food intake at a sufficient level to allow survival and growth of the lesioned rats. | 15682651 | [
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Dietary sugar and polyunsaturated fatty acid consumption as predictors of severity of schizophrenia symptoms. | A previous epidemiological study found an association between high sugar consumption and two year outcome of schizophrenia. The primary aim of the present pilot study was to assess the possible relationship between dietary sugar intake and the severity of schizophrenic symptoms in a group of 20 patients. A significant negative correlation (r = -0.54; p = 0.02) between dietary sugar and severity of schizophrenic symptoms; appears to be confounded by the effect of medication. Patients taking the antipsychotic | 15682652 | [
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Dietary sugar and polyunsaturated fatty acid consumption as predictors of severity of schizophrenia symptoms. | drug clozapine consumed almost twice as much sugar as those taking other antipsychotic agents; (235.4+/-92.6 g/day versus 143+/-59; p = 0.02) and had less severe symptoms. Consumption of other nutrients showed no association with clozapine treatment. In a secondary analysis; severity of schizophrenic symptoms correlated with dietary intake of polyunsaturated fatty acids (r = -0.54; p = 0.02) and this was independent of the effect of medication. This finding is consistent with earlier studies. | 15682652 | [
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Oxidative stress might reduce essential fatty acids in erythrocyte membranes of chronic fatigue syndrome patients. | nan | 15682653 | [
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Association of HLA phenotype with primary non-response to recombinant hepatitis B vaccine: a study from north India. | The hepatitis B vaccine is considered to be highly immunogenic and has a good safety profile. In adults; it has a primary non-response rate of 5%-10%. Causes of nonresponse to hepatitis B vaccine include age; sex; obesity smoking. Certain human leucocyte antigen (HLA) phenotypes have been known to be associated with responsiveness to the vaccine; and found to be different in different ethnic groups; such as Caucasians and Orientals. The study was designed to identify the HLA phenotypes that are associated w | 15682656 | [
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Association of HLA phenotype with primary non-response to recombinant hepatitis B vaccine: a study from north India. | ith non-responsiveness to hepatitis B virus (HBV) vaccination amongst a cohort of Indian subjects who agreed to participate in the vaccination programme. The study was offered to 107 volunteers; of whom 102 were found to be negative for HBV markers (hepatitis B surface antigen [HBsAg]; anti-HBc; anti-HBe; anti-HBs; hepatitis Be antigen [HBeAg]) . All 102 volunteers were offered recombinant hepatitis B vaccine (20 microg) at 0; 1; and 6 months. Anti-HBs antibody titres were tested on days 90 and 210 of the f | 15682656 | [
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Association of HLA phenotype with primary non-response to recombinant hepatitis B vaccine: a study from north India. | irst vaccine dose. HLA typing was done using standard microlymphotoxicity tests. The seroconversion rate of the hepatitis B vaccine was 86.3% (88/102). Fifteen nonresponders (15/102) and 15 of the 88 responders were randomly selected after age and sex matching for the purpose of studying the HLA phenotypes. HLA subtypes A1; B15; B40; A10 and DQ2 were found to be increased among nonresponders while HLA- A11; C3; DR10; DR51 (p>0.05) were the most common phenotypes amongst the responders. Further studies are n | 15682656 | [
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Association of HLA phenotype with primary non-response to recombinant hepatitis B vaccine: a study from north India. | eeded to characterize the HLA phenotypes amongst the responders in different ethnic groups in India with respect to HBV vaccination. | 15682656 | [
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Clinico-cytopathological spectrum of hepatocellular carcinoma, its correlation with serum alpha-fetoprotein level, and hepatitis B and C viral markers. | Fine-needle aspirationbiopsy (FNAB) is now widely accepted as a diagnostic modality for the treatment of hepatocellular carcinoma (HCC). The most common diagnostic problem in HCC is distinguishing it from a metastatic carcinoma. The literature from India on HCC is scanty. Hence; we studied the cytomorphological features of HCC and metastatic carcinoma. The study included 37 cases of space-occupying lesions (SOLs) of the liver as demonstrated by ultrasound or computed tomography (CT) scan. Cytomorphological | 15682657 | [
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Clinico-cytopathological spectrum of hepatocellular carcinoma, its correlation with serum alpha-fetoprotein level, and hepatitis B and C viral markers. | features of these SOLs were analyzed in all subsequent to FNAB. Hepatitis B surface antigen (HBsAg); anti-hepatitis C virus antibody (anti-HCV) and alpha-fetoprotein (AFP) were determined in all the cases by enzyme-linked immunosorbent assay (ELISA). The cytopathological diagnosis was HCC in 22 and metastatic carcinoma of the liver in 15. The individual cytomorphological features and which helped to make a definite diagnosis of HCC were: a high nuclear cytoplasmic ratio (81.8%); predominantly trabecular pat | 15682657 | [
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Clinico-cytopathological spectrum of hepatocellular carcinoma, its correlation with serum alpha-fetoprotein level, and hepatitis B and C viral markers. | tern (63.6%) and atypical naked nuclei (100%). Other features were prominent multiple nucleoli (63.3%); hyperchromasia (100%) and moderate anisonucleosis (59%). AFP was elevated in 81.8% of the cases with a mean of 634.8+812.7 ng/ml. HBsAg by ELISA was found to be positive in 72.7% of cases while only 1 case (4.5%) was positive for anti-HCV. In 1 case (4.5%); there was dual infection due to hepatitis B virus (HBV) and HCV. No viral cause was found in 18.3% of cases. | 15682657 | [
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Prevalence of Helicobactor pylori in patients with perforated duodenal ulcer. | It is believed that Helicobacter pylori (H. Pylori) plays an important role in the causation of peptic ulcer. However; its role in duodenal ulcer perforation (DUP) has not been investigated extensively and the results are conflicting. The aim of this study was to determine the prevalence of H. pylori in patients with DUP patients as compared to normal healthy volunteers (NHV). Eighty-six consecutives patients with DUP and 30 NHV were included in the study. The NHV; drawn from the Surgery outpatient; were su | 15682658 | [
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Prevalence of Helicobactor pylori in patients with perforated duodenal ulcer. | bjected to upper gastrointestinal (UGI) endoscopy and multiple gastric mucosal biopsies. Gastric mucosal biopsies among patients with DUP were obtained during laparotomy by passing a biopsy forcep through the perforation site. Biopsy specimens were put in urea broth for the rapid urease test (RUT); brucella broth (used as a transport medium) for culture and 10% formalin for histopathology. The results were analysed as the percentage of positive cases. The mean (+/-SD) age of patients with DUP (39.4+/-15.5 y | 15682658 | [
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Prevalence of Helicobactor pylori in patients with perforated duodenal ulcer. | ears) and of the NHV (36.0+/-13.9 years) were comparable (p= 0.290). Sixty-one (70.9%)patients with DUP had used NSAIDs prior to perforation. Only 7 patients with DUP (8.14%) had a past history of symptoms of duodenal ulcer and had been treated for it. A positive history of smoking; alcoholism and tobacco use was present in 68 (78.9%); 59 (68.6%) and 41(47.7%) patient with DUP; respectively. No culture was positive. Histology was positive for H. pylori in 29 patients with DUP and in 2 (6.7%)NHV. RUT was pos | 15682658 | [
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Prevalence of Helicobactor pylori in patients with perforated duodenal ulcer. | itive for H. pylori in 43(50%) patients with DUP and 5 NHV (16.7%). The present study documented a markedly higher association of H. Pylori infection with perforated duodenal ulcer. | 15682658 | [
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Clinical spectrum and natural history of non-alcoholic steatohepatitis with normal alanine aminotransferase values. | Non-alcoholic fatty liver disease (NAFLD) including nonalcoholic steatohepatitis (NASH) is a well-established cause for chronic liver disease. In most studies on NASH; elevation in alanine aminotransferase (ALT) is taken as one of the diagnostic criteria. However; the clinical and histological spectrum and natural history of NAFLD with normal ALT are not well explored. This study was planned to define the clinical spectrum and natural history of patients with NAFLD with normal ALT; and to compare them with | 15682660 | [
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] |
Clinical spectrum and natural history of non-alcoholic steatohepatitis with normal alanine aminotransferase values. | NAFLD with abnormal ALT. A prospective study including 81 consecutive patients with ahistological diagnosis ofNAFLD was planned during the period from 1999 to 2003. Consecutive (n=81) patients with the histological diagnosis of NAFLD were included in the study. In all the patients; clinical; anthropometric; laboratory; histological and imaging features were noted at the baseline. All these patients were followed up regularly at 6-month intervals. Of the 81 cases; 25 patients (including 60% cirrhotics) had p | 15682660 | [
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Clinical spectrum and natural history of non-alcoholic steatohepatitis with normal alanine aminotransferase values. | ersistently normal enzyme; whereas 56 (including 23% cirrhotics) had abnormal ALT. Both the groups were comparable with respect to distribution of age; gender; ethnicity; clinical features; imaging features; histological severity and laboratory features; except a higher incidence of diabetes and higher occurrence of advanced liver disease at baseline in NAFLD with normal ALT. Natural history of NAFLD was better in patients without cirrhosis irrespective of baseline ALT levels than in patients with cirrhosis | 15682660 | [
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Clinical spectrum and natural history of non-alcoholic steatohepatitis with normal alanine aminotransferase values. | ; except for a higher incidence of decompensation in cirrhotics with normal ALT. The entire clinical and histological spectrum of NAFLD is seen in patients with normal ALT and is not different from patients with abnormal ALT. In patients with diabetes and hepatomegaly in the absence of other obvious liver diseases; even normal ALT may not rule out advanced liver disease; and liver biopsy may be necessary to identify the severity of liver disease. | 15682660 | [
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Prevalence of metabolic syndrome in non-diabetic and non-cirrhotic patients with non-alcoholic steatohepatitis. | Recently; insulin resistance (IR) was proposed as a primary pathogenic mechanism in non-alcoholic steatohepatitis (NASH). The prevalence of IR and metabolic syndrome remains largely unstudied in patients with NASH and without diabetes and cirrhosis; both being conditions associated with IR. During a 1-year period; all non-diabetic and noncirrhotic patients seen in our institute with a diagnosis of NASH were subjected to anthropometric measurements; clinical examination; lipid profile and glucose tolerance t | 15682659 | [
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Prevalence of metabolic syndrome in non-diabetic and non-cirrhotic patients with non-alcoholic steatohepatitis. | est to define metabolic syndrome. All the patients underwent test for fasting glucose and insulin levels to define the insulin resistance by HOMA-R (homeostasis model assessment) method. Of the 25 patients with NASH; the metabolic syndrome was present in 17 (68%) and at least one criterion for the metabolic syndrome was present in all the patients. IR was present in 20 patients (80%). All the patients were either overweight (8%) or obese (92%). Because of the high prevalence of the metabolic syndrome and IR | 15682659 | [
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Prevalence of metabolic syndrome in non-diabetic and non-cirrhotic patients with non-alcoholic steatohepatitis. | in patients with NASH; it is pertinent to test for IR and metabolic abnormalities in all patients with NASH. Also; all patients with metabolic syndrome should undergo liver function tests and; in the presence of abnormal transaminases; a liver biopsy to define NASH. | 15682659 | [
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Gastro-oesophageal reflux disease in Sudan: a clinical endoscopic and histopathological study. | This study was conducted to assess the frequency of gastroesophageal reflux disease (GERD) and Barrett's esophagus among Sudanese patients with clinical symptoms of heartburn. One hundred and five patients were included in the study; forty seven patients had evidence of reflux oesophagitis; 61.7% of whom had grade B oesophagitis according to the modified Los Angeles classification and 10.6% had Barrett's oesophagus. 78.7% of the biopsies from the esophageal cardia revealed presenced of inflammation (Carditi | 15682661 | [
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Gastro-oesophageal reflux disease in Sudan: a clinical endoscopic and histopathological study. | s). Dysplasia was documented in 21.3% of these biopsies. Helicobacter pylori was detected 59.6% of gastrooesophageal reflux disease patients and 56.8% of patients with carditis. However; 80% of patients with Barrett oesophagus were positive for Helicobacter pylori. It was concluded that gastro-oesophageal reflux disease affects all age groups with males being affected more than females and Helicobacter pylori infection did not play a major role in gastro-oesophageal reflux disease orits complications. | 15682661 | [
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Type 1 diabetes mellitus and extrahepatic portal vein obstruction: a causal association? | So far; no association has been described between multifactiorial disorders such as insulin- dependent diabetes mellitus and extrahepatic portal vein obstruction (EHPVO); which is the most common cause of extra-hepatic portal hypertension in children. We describe 3 cases of EHPVO who developed type 1 diabetes mellitus. | 15682662 | [
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Peritoneal transplantation of human fetal hepatocytes for the treatment of acute fatty liver of pregnancy: a case report. | We report the case of a 26-year-old second gravida in the third trimester of pregnancy who presented with a history of nausea; repeated vomiting and jaundice. The patient was diagnosed as acute fatty liver of pregnancy. After delivery; the condition of the patient progressed to grade IV encephalopathy and did not improve despite all intensive clinical management measures. After 3 days in grade IV encephalopathy; the patient was infused 3 x 10(8) human foetal hepatocytes. The patient's level of consciousness | 15682663 | [
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Peritoneal transplantation of human fetal hepatocytes for the treatment of acute fatty liver of pregnancy: a case report. | started improving after 24 hours of foetal hepatocyte transfusion and she recovered completely within 7 days. | 15682663 | [
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The tipping point tango or, it takes more than two to do this tango. | nan | 15682668 | [
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Severe acute bleeding from portal colopathy controlled by somatostatin: a case report. | Portal hypertensive colopathy (PHC) is a recently described entity in patients with portal hypertension which can cause even life-threatening lower gastrointestinal bleeding. In contrast to variceal bleed; there is no standardized treatment for the control of bleeding from these lesions. We report a case of alcoholic cirrhosis with portal hypertension; in whom bleeding from colonic angiodysplasia-like lesions was effectively controlled by somatostatin infusion. | 15682664 | [
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Everything you always wanted to know about software escrow agreements--and then some! | nan | 15682669 | [
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Traction as a cause of gallbladder avulsion. | Following isolated blunt trauma to the abdomen; a 25-year-old man developed peritonitis. Laparotomy revealed a band between the transverse colon and gallbladder; with partial avulsion of the latter. Avulsion of the gallbladder is a rare injury and; to the best of our knowledge traction has not been described as a mechanism of injury. | 15682665 | [
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Successful healthcare programs and projects: organization portfolio management essentials. | Many healthcare organization projects take more time and resources than planned and fail to deliver desired business outcomes. Healthcare IT is a major component of many projects and often undeservedly receives the blame for failure. Poor results are often not a result of faulty healthcare IT or poor project management or poor project execution alone. Many projects fail because of poor portfolio management--poor planning and management of the portfolio of initiatives designed to meet an organization's strat | 15682672 | [
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Successful healthcare programs and projects: organization portfolio management essentials. | egic goals. Because resources are limited; portfolio management enables organizations to more strategically allocate and manage their resources so care delivery; service delivery; and initiatives that advance organizations toward their strategic goals; including healthcare IT initiatives; can be accomplished at the levels of quality and service desired by an organization. Proper portfolio management is the essential foundation for program and project success and supports overall organization success. Withou | 15682672 | [
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Successful healthcare programs and projects: organization portfolio management essentials. | t portfolio management; even programs and projects that execute flawlessly may not meet desired objectives. This article discusses the essential requirements for porfolio management. These include opportunity identification; return on investment (ROI) forecast; project prioritization; capacity planning (inclusive of human; financial; capital; and facilities resources); work scheduling; program and project management and execution; and project performance and value assessment. Portfolio management is essenti | 15682672 | [
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Successful healthcare programs and projects: organization portfolio management essentials. | al to successful healthcare project execution. Theories are drawn from the Organizational Project Management Maturity Model (OPM3) work of the Project Management Institute and other leading strategy; planning; and organization change management research institutes. | 15682672 | [
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The standing operational implementation team--playing a vital role in successful project completion, implementation of operational initiatives, and process improvement. | The most detailed and well-documented project plan or process improvement initiative will certainly fall short of expectations if the appropriate team is not assembled to execute it. A standing operational implementation team; representing all major stakeholders in the organization; takes the guesswork out of who to engage and when. Applying this concept; as well as some basic project management concepts; can be used to help guide facilitators who are not project management professionals toward successful p | 15682673 | [
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Healthcare technology project ownership. | Hospitals have great expectations that technology will address their critical strategic issues. However; obtaining satisfactory results--or having confidence that it can be done well--has been a challenge. Most hospital executives acknowledge that success is not primarily a matter of whether or not the technology is capable of performing required functions; rather; it is a matter of whether people will use the new technology to improve performance. While care delivery; patient safety; and hospital margins s | 15682674 | [
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Healthcare technology project ownership. | tand to gain from the increased use of automation and electronic information; these are not actually technology projects. Instead; these are process breakthroughs that are enabled by technology that yield performance improvements. Hospital clinical and administrative leaders own the results and must take active ownership of projects. This paradigm shift begins when project sponsors have a clear understanding of their role in technology-based change initiatives. | 15682674 | [
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Lean sigma--will it work for healthcare? | The manufacturing industry has been using Lean Sigma for years in pursuit of continuous improvement to obtain a competitive advantage. The objectives of these efforts are to use the Lean techniques for reducing cycle times and the Six Sigma concepts for reducing product defects. The Iowa Business Council with several advocates worked with the University of Iowa Hospital and Clinics (UIHC) and two other Iowa hospitals to determine whether Lean Sigma is adaptable in healthcare. A team of 15 people at UIHC use | 15682675 | [
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Lean sigma--will it work for healthcare? | d the Kaizen Breakthrough Methodology over a five-day period in an aggressive identification and elimination of non-value added activities in Radiology CT scanning. The results exceeded the initial project objectives and indicated that Lean Sigma is applicable in healthcare. Overall; the Lean Sigma project increased revenue by approximately $750;000 per year. The Kaizen process proved to be successful and interesting. Within three days; the team installed new work flow processes. This implementation-oriente | 15682675 | [
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Lean sigma--will it work for healthcare? | d approach is what differentiates Lean Sigma from other quality improvement processes. | 15682675 | [
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Facilitating applied information technology research. | Most healthcare organizations need to develop an understanding of the role; maturity; and utility of new information technologies. Performing this research can present a series of challenges. For example; IT staff may not know how to obtain resources; the research may show promising results but fail to be implemented; and organizations may not develop a thoughtful portfolio of research projects. To minimize these problems; applied information technology research must be managed. This paper describes the inf | 15682676 | [
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Facilitating applied information technology research. | ormation technology research process at Partners HealthCare. The research process is a management process designed to identify important research topics; solicit IT staff proposals to investigate topics; prioritize and fund research projects; and communicate and disseminate research results. Sample research topics include the role of RFID; the impact of Internet-based e-visits; and approaches for integrating speech and direct input for clinical information systems. The paper describes the goals and activiti | 15682676 | [
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Facilitating applied information technology research. | es of the research process and the role of the research council. The research proposal review process is discussed; and an inventory of approved research projects is reviewed. The paper discusses several challenges involved in creating and managing the research process. | 15682676 | [
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