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The CML-related oncoprotein BCR/ABL induces expression of histidine decarboxylase (HDC) and the synthesis of histamine in leukemic cells. | e and terfenadine; which bind to CYP450; were found to counteract proliferation of CML cells; whereas no growth inhibition was observed with the HR-1 antagonist fexofenadine which is not targeted or metabolized by CYP450. Moreover; DPPE; an inhibitor of histamine-binding CYP450 isoenzymes; produced growth inhibition in CML cells. Together; these data show that BCR/ABL promotes histamine production in CML cells and that certain HR-targeting drugs exert antileukemic effects on CML cells. | 16849647 | [
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Rules for modeling signal-transduction systems. | Formalized rules for protein-protein interactions have recently been introduced to represent the binding and enzymatic activities of proteins in cellular signaling. Rules encode an understanding of how a system works in terms of the biomolecules in the system and their possible states and interactions. A set of rules can be as easy to read as a diagrammatic interaction map; but unlike most such maps; rules have precise interpretations. Rules can be processed to automatically generate a mathematical or compu | 16849649 | [
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Rules for modeling signal-transduction systems. | tational model for a system; which enables explanatory and predictive insights into the system's behavior. Rules are independent units of a model specification that facilitate model revision. Instead of changing a large number of equations or lines of code; as may be required in the case of a conventional mathematical model; a protein interaction can be introduced or modified simply by adding or changing a single rule that represents the interaction of interest. Rules can be defined and visualized by using | 16849649 | [
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Rules for modeling signal-transduction systems. | graphs; so no specialized training in mathematics or computer science is necessary to create models or to take advantage of the representational precision of rules. Rules can be encoded in a machine-readable format to enable electronic storage and exchange of models; as well as basic knowledge about protein-protein interactions. Here; we review the motivation for rule-based modeling; applications of the approach; and issues that arise in model specification; simulation; and testing. We also discuss rule vis | 16849649 | [
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Short-chain fatty acids induce gamma-globin gene expression by displacement of a HDAC3-NCoR repressor complex. | High-level induction of fetal (gamma) globin gene expression for therapy of beta-hemoglobinopathies likely requires local chromatin modification and dissociation of repressor complexes for gamma-globin promoter activation. A novel gamma-globin-inducing short-chain fatty acid derivative (SCFAD); RB7; which was identified through computational modeling; produced a 6-fold induction in a reporter assay that detects only strong inducers of the gamma-globin gene promoter and in cultured human erythroid progenitor | 16849648 | [
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Short-chain fatty acids induce gamma-globin gene expression by displacement of a HDAC3-NCoR repressor complex. | s. To elucidate the molecular mechanisms used by high-potency SCFADs; chromatin immunoprecipitation (ChIP) assays performed at the human gamma- and beta-globin gene promoters in GM979 cells and in erythroid progenitors demonstrate that RB7 and butyrate induce dissociation of HDAC3 (but not HDAC1 or HDAC2) and its adaptor protein NCoR; specifically from the gamma-globin gene promoter. A coincident and proportional recruitment of RNA polymerase II to the gamma-globin gene promoter was observed with exposure t | 16849648 | [
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Short-chain fatty acids induce gamma-globin gene expression by displacement of a HDAC3-NCoR repressor complex. | o these gamma-globin inducers. Knockdown of HDAC3 by siRNA induced transcription of the gamma-globin gene promoter; demonstrating that displacement of HDAC3 from the gamma-globin gene promoter by the SCFAD is sufficient to induce gamma-globin gene expression. These studies demonstrate new dynamic alterations in transcriptional regulatory complexes associated with SCFAD-induced activation of the gamma-globin gene and provide a specific molecular target for potential therapeutic intervention. | 16849648 | [
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Are placebos in advanced cancer trials ethically justified? | nan | 16849652 | [
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Migraine and risk of cardiovascular disease in women. | Migraine with aura has been associated with an adverse cardiovascular risk profile and prothrombotic factors that; along with migraine-specific physiology; may increase the risk of vascular events. Although migraine with aura has been associated with increased risk of ischemic stroke; an association with cardiovascular disease (CVD) and; specifically; coronary events remains unclear.To evaluate the association between migraine with and without aura and subsequent risk of overall and specific CVD.Prospective | 16849661 | [
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Migraine and risk of cardiovascular disease in women. | cohort study of 27;840 US women aged 45 years or older who were participating in the Women's Health Study; were free of CVD and angina at study entry (1992-1995); and who had information on self-reported migraine and aura status; and lipid measurements. This report is based on follow-up data through March 31; 2004.The primary outcome measure was the combined end point of major CVD (first instance of nonfatal ischemic stroke; nonfatal myocardial infarction; or death due to ischemic CVD); other measures were | 16849661 | [
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Migraine and risk of cardiovascular disease in women. | first ischemic stroke; myocardial infarction; coronary revascularization; angina; and death due to ischemic CVD.At baseline; 5125 women (18.4%) reported any history of migraine; of the 3610 with active migraine (migraine in the prior year); 1434 (39.7%) indicated aura symptoms. During a mean of 10 years of follow-up; 580 major CVD events occurred. Compared with women with no migraine history; women who reported active migraine with aura had multivariable-adjusted hazard ratios of 2.15 (95% confidence inter | 16849661 | [
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Migraine and risk of cardiovascular disease in women. | val [CI]; 1.58-2.92; P<.001) for major CVD; 1.91 (95% CI; 1.17-3.10; P = .01) for ischemic stroke; 2.08 (95% CI; 1.30-3.31; P = .002) for myocardial infarction; 1.74 (95% CI; 1.23-2.46; P = .002) for coronary revascularization; 1.71 (95% CI; 1.16-2.53; P = .007) for angina; and 2.33 (95% CI; 1.21-4.51; P = .01) for ischemic CVD death. After adjusting for age; there were 18 additional major CVD events attributable to migraine with aura per 10 000 women per year. Women who reported active migraine without aur | 16849661 | [
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Migraine and risk of cardiovascular disease in women. | a did not have increased risk of any vascular events or angina.In this large; prospective cohort of women; active migraine with aura was associated with increased risk of major CVD; myocardial infarction; ischemic stroke; and death due to ischemic CVD; as well as with coronary revascularization and angina. Active migraine without aura was not associated with increased risk of any CVD event. | 16849661 | [
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Incidence of opportunistic and other infections in HIV-infected children in the HAART era. | Combination anti-retroviral therapy or highly active antiretroviral therapy (HAART) has resulted in a dramatic decline in the incidence of opportunistic and other infections in human immunodeficiency virus (HIV)-infected adults and children.To estimate the incidence of 29 targeted opportunistic and other infections occurring in the era of HAART-between January 1; 2001; and December 31; 2004-in HIV-infected infants; children; and adolescents followed up in Pediatric AIDS Clinical Trials Group (PACTG) 219C; t | 16849662 | [
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Incidence of opportunistic and other infections in HIV-infected children in the HAART era. | o compare incidence rates in the HAART era to those of the pre-HAART era; and to test for linear trends over time in the HAART era.Ongoing; multicenter; prospective cohort study designed to examine long-term outcomes in HIV-infected children. The study population included 2767 children enrolled between September 15; 2000; and December 31; 2004; with information entered in the database up to August 1; 2005; when data analysis was conducted. The pre-HAART era comparison population included 3331 children enrol | 16849662 | [
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Incidence of opportunistic and other infections in HIV-infected children in the HAART era. | led in 13 PACTG protocols from October 1988 to August 1998.First occurrence of each of the 29 targeted infections.Seventy-five percent of the children were enrolled in 2000 and 2001; 90% acquired HIV perinatally; 52% were girls; and 59% were black. The median age was 8.2 years (range; 6-13 years). The median duration of follow-up was 3.4 years. Overall; 553 first episodes of a specific infection occurred among 395 (14%) of the study participants. The number of events for the 4 most common first-time infecti | 16849662 | [
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Incidence of opportunistic and other infections in HIV-infected children in the HAART era. | ons and their incidence rates (IRs) per 100 person-years were 123 bacterial pneumonia (IR; 2.15; 95% confidence interval [CI]; 1.79-2.56); 77 herpes zoster (IR; 1.11; 95% CI; 0.88-1.39); 57 dermatophyte infections (IR; 0.88; 0.67-1.14); and 52 oral candidiasis (IR; 0.93; 95% CI; 0.70-1.22). Incidence rates of first bacteremia; Pneumocystis jeroveci pneumonia; disseminated Mycobacterium avium complex; lymphoid interstitial pneumonitis; systemic fungal infection; cytomegalovirus retinitis; and tuberculosis we | 16849662 | [
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Incidence of opportunistic and other infections in HIV-infected children in the HAART era. | re all less than 0.50 per 100 person-years. There were no statistically significant linear trends in incidence for any of the 29 infections over the 4 calendar years. However; infection rates were significantly lower than those reported in the PACTG in the pre-HAART era. The pre-HAART IRs were as follows: for bacterial pneumonia; IR; 11.1; 95% CI; 10.3-12.0; bacteremia; IR; 3.3; 95% CI; 2.9-3.8; herpes zoster; IR; 2.9; 95% CI; 2.6-3.3; disseminated M avium complex; IR; 1.8; 95% CI; 1.5-2.1; P jeroveci; IR; | 16849662 | [
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Incidence of opportunistic and other infections in HIV-infected children in the HAART era. | 1.3; 95% CI; 1.1-1.6; oral candidiasis; IR; 1.2; 95% CI; 1.0-1.5; cytomegalovirus retinitis; IR; 0.5; 95% CI; 0.3-0.6; and tuberculosis; IR; 0.2; 95% CI; 0.1-0.4.Opportunistic infections and other related infections are uncommon in children in the HAART era; and infection rates continue to be lower than those reported in the pre-HAART era. Continued surveillance is important to assess the long-term effect of HAART on the occurrence of opportunistic and other related infections in children. | 16849662 | [
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Complement factor H polymorphism, complement activators, and risk of age-related macular degeneration. | The evidence that inflammation is an important pathway in age-related macular degeneration (AMD) is growing. Recent case-control studies demonstrated an association between the complement factor H (CFH) gene; a regulator of complement; and AMD.To assess the associations between the CFH gene and AMD in the general population and to investigate the modifying effect of smoking; serum inflammatory markers; and genetic variation of C-reactive protein (CRP).Population-based; prospective cohort study of individual | 16849663 | [
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Complement factor H polymorphism, complement activators, and risk of age-related macular degeneration. | s aged 55 years or older (enrollment between March 20; 1990; and July 31; 1993; and 3 follow-up examinations that were performed between September 1; 1993; and December 31; 2004) in Rotterdam; the Netherlands. The CFH Y402H polymorphism was determined in a total of 5681 individuals. Information on smoking; erythrocyte sedimentation rate; CRP serum levels; and haplotypes of the CRP gene were assessed at baseline.All severity stages of prevalent and incident AMD; graded according to the international classifi | 16849663 | [
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Complement factor H polymorphism, complement activators, and risk of age-related macular degeneration. | cation and grading system for AMD.The frequency of CFH Y402H was 36.2% (4116/11;362 alleles). At baseline; there were 2062 persons (36.3%) with any type of AMD (prevalent cases); including 78 (1.4%) with late AMD (stage 4). During follow-up (mean; 8 years; median; 10 years); 1649 (35.5%) of 4642 participants progressed to a higher stage of AMD (incident cases); including 93 (5.6%) who developed late AMD. The odds ratio (OR) of AMD increased in an allele-dose manner with 2.00 (95% confidence interval [CI]; 1 | 16849663 | [
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Complement factor H polymorphism, complement activators, and risk of age-related macular degeneration. | .56-2.55) for stage 2 AMD; 4.58 (95% CI; 2.82-7.44) for stage 3 AMD; and 11.02 (95% CI; 6.82-11.81) for stage 4 (late; vision threatening) AMD for homozygous persons. Cumulative risks calculated by Kaplan-Meier analysis of late AMD by age 95 years were 48.3% for homozygotes; 42.6% for heterozygotes; and 21.9% for noncarriers. The population-attributable risk for CFH Y402H was 54.0%. Elevated erythrocyte sedimentation rates further increased the OR to 20.2 (95% CI; 9.5-43.0); elevated serum CRP levels to 27. | 16849663 | [
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Complement factor H polymorphism, complement activators, and risk of age-related macular degeneration. | 7 (95% CI; 10.7-72.0); and smoking to 34.0 (95% CI; 13.0-88.6) for homozygotes compared with noncarriers without these determinants. The CRP haplotypes conferring high levels of CRP significantly increased the effect of CFH Y402H (P<.01).The CFH Y402H polymorphism may account for a substantial proportion of AMD in individuals similar to those in the Rotterdam Study and may confer particular risk in the presence of environmental and genetic stimulators of the complement cascade. | 16849663 | [
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N-terminal pro-brain natriuretic peptide levels and risk of death in sickle cell disease. | Thirty percent of patients with sickle cell disease (SCD) develop pulmonary hypertension; a major risk factor for death in this population. A validated blood biomarker of pulmonary hypertension in SCD could provide important prognostic and diagnostic information and allow the exploration of the prevalence of pulmonary hypertension in participants in the 1996 Multicenter Study of Hydroxyurea in Sickle Cell Anemia (MSH) Patients' Follow-up Study. Levels of N-terminal pro-brain natriuretic peptide (NT-proBNP) | 16849664 | [
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N-terminal pro-brain natriuretic peptide levels and risk of death in sickle cell disease. | provide such information in patients with idiopathic pulmonary arterial hypertension.To determine the relationship between NT-proBNP levels and severity of pulmonary hypertension and prospective mortality in patients with SCD.NT-proBNP levels were measured in 230 participants in the National Institutes of Health (NIH) Sickle Cell Disease-Pulmonary Hypertension Screening Study (enrollment between February 2001 and March 2005) and in 121 samples from patients enrolled starting in 1996 in the MSH Patients' Fol | 16849664 | [
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N-terminal pro-brain natriuretic peptide levels and risk of death in sickle cell disease. | low-up Study. A threshold level predictive of high pulmonary artery pressure and mortality was identified in the NIH Sickle Cell Disease-Pulmonary Hypertension Screening Study and used to define an a priori analytical plan to determine the prevalence and associated mortality of pulmonary hypertension in the MSH follow-up study.Severity of pulmonary hypertension and risk of all-cause mortality.NT-proBNP levels were higher in patients with sickle cell pulmonary hypertension and correlated directly with tricus | 16849664 | [
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N-terminal pro-brain natriuretic peptide levels and risk of death in sickle cell disease. | pid regurgitant jet velocity in the NIH cohort (R = 0.50; P<.001). An NT-proBNP level of 160 pg/mL or greater had a 78% positive predictive value for the diagnosis of pulmonary hypertension and was an independent predictor of mortality (21 deaths at 31 months' median follow-up; risk ratio; 5.1; 95% confidence interval; 2.1-12.5; P<.001; 19.5% absolute increase in risk of death). In the MSH cohort; 30% of patients had an NT-proBNP level of 160 pg/mL or greater. An NT-proBNP level of 160 pg/mL or greater in t | 16849664 | [
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N-terminal pro-brain natriuretic peptide levels and risk of death in sickle cell disease. | he MSH cohort was independently associated with mortality by Cox proportional hazards regression analysis (24 deaths at 47 months' median follow-up; risk ratio; 2.87; 95% confidence interval; 1.2-6.6; P = .02; 11.9% absolute increase in risk of death).Pulmonary hypertension; as indicated by an NT-proBNP level of 160 pg/mL or greater; was very common in patients in the NIH study and in the MSH cohort. The MSH analysis suggests that rates of vaso-occlusive pain episodes in these patients were unrelated to ris | 16849664 | [
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A 39-year-old woman with hypercholesterolemia. | Ms T; a 39-year-old woman; has a total cholesterol level of 277 mg/dL (7.17 mmol/L) and well-controlled hypertension; her brother had a stroke in his 30s. She is primarily concerned with her mother's history of breast cancer; but she would like to know if she can take a dietary supplement or if she needs to take cholesterol-lowering medication; and if so; whether she will need to continue it as well as adhere to her diet for the rest of her life. Her estimated 10-year risk of developing coronary heart disea | 16849665 | [
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A 39-year-old woman with hypercholesterolemia. | se (CHD) is 1% to 2% using the Framingham Risk Score; but that may underestimate her true risk as an African American woman with a family history of CHD. Recommendations for her; her longer-term risk for CHD; and evidence for lipid-lowering therapies are discussed. | 16849665 | [
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(Nutritional) chemoprevention of cancer: what's up? | nan | 16849669 | [
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Choking hypoxia-inducible factor 1alpha: a novel mechanism for connective tissue growth factor inhibition of angiogenesis. | nan | 16849670 | [
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Breast cancer heterogeneity: a mixture of at least two main types? | nan | 16849671 | [
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Phase I antibody risks, trial safety examined. | nan | 16849675 | [
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Ten years later: liver cancer treatment reevaluated. | nan | 16849677 | [
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Split prostate cancer four ways, Willett says. | nan | 16849678 | [
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Nutritional interventions and outcome in patients with cancer or preinvasive lesions: systematic review. | Dietary modifications and supplements are used widely by patients with cancer and preinvasive lesions as an adjunct to standard treatment. Given the widespread use of nutritional modifications and supplements by such patients and concerns about the lack of benefit and possible harm; we conducted a systematic review of randomized controlled trials to examine the effect of nutritional interventions on patients with cancer or preinvasive lesions.We searched electronic databases and reference lists to locate al | 16849679 | [
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Nutritional interventions and outcome in patients with cancer or preinvasive lesions: systematic review. | l eligible trials and analyzed trial quality. Outcome measures were all-cause and cancer mortality; disease-free survival; cancer recurrence; second primary cancer; recurrence of a preinvasive lesion; or progression to cancer. Results of individual trials were combined by use of random-effects meta-analyses.We identified 59 eligible trials; 25 in patients with cancer and 34 in patients with preinvasive lesions; respectively. Trial quality was generally low; only three trials (two of cancer and one of preinv | 16849679 | [
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Nutritional interventions and outcome in patients with cancer or preinvasive lesions: systematic review. | asive lesions) had adequate methods for generating the allocation sequence; allocation concealment; and masking both outcome assessors and participants. The combined odds ratio (OR) for the effect of a healthy diet-given alone or with dietary supplements; weight loss; or exercise-on all-cause mortality was 0.90 (95% confidence interval [CI] = 0.46 to 1.77). There was no evidence of an association between the use of antioxidant (OR = 1.01; 95% CI = 0.88 to 1.15) or retinol (OR = 0.97; 95% CI = 0.83 to 1.13) | 16849679 | [
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Nutritional interventions and outcome in patients with cancer or preinvasive lesions: systematic review. | supplements and all-cause mortality. Meta-analyses of all other outcomes did not show clear evidence of benefit or harm.The impact of most nutritional interventions cannot be reliably estimated because of the limited number of trials; many of which were of low quality. There is no evidence that dietary modification by cancer patients improves survival and benefits disease prognosis. | 16849679 | [
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Randomized double-blind factorial trial of three treatments to reduce the prevalence of precancerous gastric lesions. | Randomized trials have yielded mixed results on the effects of treatment for Helicobacter pylori and little information on the effects of vitamins or garlic supplements on precancerous gastric lesions. We conducted a randomized trial to test the effects of one-time H. pylori treatment and long-term vitamin or garlic supplements in reducing the prevalence of advanced precancerous gastric lesions.Most of the adults aged 35-64 years in 13 randomly selected villages in Linqu County; Shandong Province; China; we | 16849680 | [
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Randomized double-blind factorial trial of three treatments to reduce the prevalence of precancerous gastric lesions. | re identified and given baseline endoscopies in 1994. In 1995; 3365 eligible subjects were randomly assigned in a factorial design to three interventions or placebos: amoxicillin and omeprazole for 2 weeks in 1995 (H. pylori treatment); vitamin C; vitamin E; and selenium for 7.3 years (vitamin supplement); and aged garlic extract and steam-distilled garlic oil for 7.3 years (garlic supplement). Subjects underwent endoscopies with biopsies in 1999 and 2003; and the prevalence of precancerous gastric lesions | 16849680 | [
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Randomized double-blind factorial trial of three treatments to reduce the prevalence of precancerous gastric lesions. | ment resulted in statistically significant decreases in the combined prevalence of severe chronic atrophic gastritis; intestinal metaplasia; dysplasia; or gastric cancer in 1999 (odds ratio [OR] = 0.77; 95% confidence interval [CI] = 0.62 to 0.95) and in 2003 (OR = 0.60; 95% CI = 0.47 to 0.75); and had favorable effects on the average histopathologic severity and on progression and regression of precancerous gastric lesions in 2003. H. pylori treatment did not reduce the combined prevalence of dysplasia or | 16849680 | [
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Randomized double-blind factorial trial of three treatments to reduce the prevalence of precancerous gastric lesions. | gastric cancer. However; fewer subjects receiving H. pylori treatment (19/1130; 1.7%) than receiving placebo (27/1128; 2.4%) developed gastric cancer (adjusted P = .14). No statistically significant favorable effects were seen for garlic or vitamin supplements.H. pylori treatment reduces the prevalence of precancerous gastric lesions and may reduce gastric cancer incidence; but further data are needed to prove the latter point. Long-term vitamin or garlic supplementation had no beneficial effects on the pre | 16849680 | [
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Randomized double-blind factorial trial of three treatments to reduce the prevalence of precancerous gastric lesions. | valence of precancerous gastric lesions or on gastric cancer incidence. | 16849680 | [
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Effect of connective tissue growth factor on hypoxia-inducible factor 1alpha degradation and tumor angiogenesis. | Connective tissue growth factor (CTGF) inhibits the metastatic activity of human lung cancer cells in a mouse model; however; the mechanism of this modulation is unclear. We investigated the role of angiogenesis in this process.CL1-5 and A549 human lung adenocarcinoma cells were stably transfected with vectors containing CTGF or hypoxia-inducible factor (HIF) 1alpha or with vector controls. Transfected cells were injected into nude mice (n = 10 per group); and tumor growth; metastasis; and mouse survival we | 16849681 | [
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Effect of connective tissue growth factor on hypoxia-inducible factor 1alpha degradation and tumor angiogenesis. | re measured. Excised xenograft tumors and primary human lung adenocarcinomas (n = 24) were subjected to immunohistochemistry with antibodies to the endothelial cell marker CD31 and to CTGF. Expression of HIF-1alpha and vascular endothelial growth factor (VEGF) A was assessed in vitro by using reporter gene assays. Cells were transiently transfected with HIF-1alpha mutant and antisense arrest-defective 1 protein (ARD-1); and HIF-1alpha acetylation was assayed by immunoprecipitation. All statistical tests wer | 16849681 | [
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Effect of connective tissue growth factor on hypoxia-inducible factor 1alpha degradation and tumor angiogenesis. | e two-sided.Xenograft tumors derived from CTGF transfectants grew more slowly than those from control-transfected cells and had reduced expression of HIF-1alpha and VEGF-A; vascularization (as assessed by CD31 expression); and metastasis (all P<.001). Xenograft tumors derived from CTGF-overexpressing cells that were transfected with HIF-1alpha had higher VEGF-A expression than CTGF-overexpressing xenografts. Mice with CTGF/HIF-1alpha xenografts had lower survival than mice carrying CTGF-overexpressing xenog | 16849681 | [
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Effect of connective tissue growth factor on hypoxia-inducible factor 1alpha degradation and tumor angiogenesis. | rafts (CL1-5/Neo; mean = 69.6 days; 95% confidence interval [CI] = 53.9 to 85.3 days versus CL1-5/CTGF; mean = 102.1 days; 95% CI = 92.1 to 112.1 days; P = .001; CL1-5/CTGF; mean = 102.1 days; 95% CI = 92.1 to 112.1 days versus CL1-5/CTGF/HIF-1alpha; mean = 81.7 days; 95% CI = 66.5 to 96.9 days; P = .011; CL1-5/Neo; mean = 69.6 days; 95% CI = 53.9 to 85.3 days versus CL1-5/CTGF/HIF-1alpha; mean = 81.7 days; 95% CI = 66.5 to 96.9 days; P = .122). Tumors of patients with the same disease stage but with high C | 16849681 | [
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Effect of connective tissue growth factor on hypoxia-inducible factor 1alpha degradation and tumor angiogenesis. | TGF protein expression had reduced microvessel density compared with tumors with low expression. Transfection with antisense-ARD1 decreased the level of acetylated HIF-1alpha and restored HIF-1alpha and VEGF-A expression in CTGF-overexpressing cells.CTGF inhibition of metastasis involves the inhibition of VEGF-A-dependent angiogenesis; possibly by promoting HIF-1alpha protein degradation. | 16849681 | [
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Quantitation of promoter methylation of multiple genes in urine DNA and bladder cancer detection. | The noninvasive identification of bladder tumors may improve disease control and prevent disease progression. Aberrant promoter methylation (i.e.; hypermethylation) is a major mechanism for silencing tumor suppressor genes and other cancer-associated genes in many human cancers; including bladder cancer.A quantitative fluorogenic real-time polymerase chain reaction (PCR) assay was used to examine primary tumor DNA and urine sediment DNA from 15 patients with bladder cancer and 25 control subjects for promot | 16849682 | [
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Quantitation of promoter methylation of multiple genes in urine DNA and bladder cancer detection. | er hypermethylation of nine genes (APC; ARF; CDH1; GSTP1; MGMT; CDKN2A; RARbeta2; RASSF1A; and TIMP3) to identify potential biomarkers for bladder cancer. We then used these markers to examine urine sediment DNA samples from an additional 160 patients with bladder cancers of various stages and grades and from an additional 69 age-matched control subjects. Data were analyzed on the basis of a prediction model and were internally validated using a jacknife procedure. All statistical tests were two-sided.For a | 16849682 | [
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Quantitation of promoter methylation of multiple genes in urine DNA and bladder cancer detection. | ll 15 patients with paired DNA samples; the promoter methylation pattern in urine matched that in the primary tumors. Four genes displayed 100% specificity. Of the 175 bladder cancer patients; 121 (69%; 95% confidence interval [CI] = 62% to 76%) displayed promoter methylation in at least one of these genes (CDKN2A; ARF; MGMT; and GSTP1); whereas all control subjects were negative for such methylation (100% specificity; 95% CI = 96% to 100%). A logistic prediction model using the methylation levels of all re | 16849682 | [
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Quantitation of promoter methylation of multiple genes in urine DNA and bladder cancer detection. | maining five genes was developed and internally validated for subjects who were negative on the four-gene panel. This combined; two-stage predictor produced an internally validated ROC curve with an overall sensitivity of 82% (95% CI = 75 % to 87%) and specificity of 96% (95% CI = 90% to 99%).Testing a small panel of genes with the quantitative methylation-specific PCR assay in urine sediment DNA is a powerful noninvasive approach for the detection of bladder cancer. Larger independent confirmatory cohorts | 16849682 | [
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Quantitation of promoter methylation of multiple genes in urine DNA and bladder cancer detection. | with longitudinal follow-up will be required in future studies to define the impact of this technology on early detection; prognosis; and disease monitoring before clinical application. | 16849682 | [
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Serum concentrations of 1,1,1-trichloro-2,2-bis(p-chlorophenyl)ethane (DDT) and 1,1-dichloro-2,2-bis(p-chlorophenyl)ethylene (DDE) and risk of primary liver cancer. | 1;1;1-Trichloro-2;2-bis(p-chlorophenyl)ethane (DDT) exposure has been demonstrated to cause liver tumors in laboratory rodents. DDT's persistent metabolite and environmental degradation product; 1;1-dichloro-2;2-bis(p-chlorophenyl)ethylene (DDE); has also been associated with liver tumors in laboratory animals. Whether DDT and DDE are associated with hepatocarcinogenesis in humans is not clear.We carried out a nested case-control study among the participants of the Nutritional Intervention Trials in Linxian | 16849683 | [
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Serum concentrations of 1,1,1-trichloro-2,2-bis(p-chlorophenyl)ethane (DDT) and 1,1-dichloro-2,2-bis(p-chlorophenyl)ethylene (DDE) and risk of primary liver cancer. | ; China. The case group included 168 individuals who developed liver cancer during the trials; and the control group included 385 individuals frequency-matched on age and sex who were alive and well at the end of the study. Serum concentrations of DDT and DDE were measured by gas chromatography-mass spectrometry. Odds ratios (ORs) and 95% confidence intervals (CIs) were calculated using multivariable analysis.In multivariable-adjusted models; the risk of developing liver cancer increased with increased seru | 16849683 | [
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Serum concentrations of 1,1,1-trichloro-2,2-bis(p-chlorophenyl)ethane (DDT) and 1,1-dichloro-2,2-bis(p-chlorophenyl)ethylene (DDE) and risk of primary liver cancer. | m DDT concentration (OR for quintile 1 versus quintile 5 = 3.8; 95% CI = 1.7 to 8.6; P(trend) = .0024). In contrast; there was no statistically significant association between liver cancer and serum DDE concentration. The association between high serum DDT concentration and liver cancer was stronger among individuals with DDE concentrations below the median value (odds ratio for tertile 3 versus tertile 1 = 3.55; 95% CI = 1.45 to 8.74) than those with concentrations above the median (OR = 1.70; 95% CI = 0.9 | 16849683 | [
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Serum concentrations of 1,1,1-trichloro-2,2-bis(p-chlorophenyl)ethane (DDT) and 1,1-dichloro-2,2-bis(p-chlorophenyl)ethylene (DDE) and risk of primary liver cancer. | 7 to 2.98). A calculation of crude liver cancer risk found that there would be 26 liver cancers per 100 000 persons per year in the lowest quintile of DDT exposure versus 46 liver cancers per 100 000 persons per year in the highest quintile of DDT exposure.DDT may be a risk factor for liver cancer; particularly among persons with lower DDE concentrations. Risk may be particularly increased among persons exposed directly to DDT (resulting in a higher ratio of DDT to DDE) or; alternatively; risk may be associ | 16849683 | [
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Steroid hormone receptor status of mouse mammary stem cells. | The estrogen receptor alpha (ERalpha); progesterone receptor (PR); and erbB2 (Her2 in humans) are important prognostic markers of human breast cancer; and they are variably expressed in different subtypes of breast cancer. The basal subtype; for example; is negative for ERalpha; PR; and Her2 by immunohistochemistry. We investigated the expression of these signaling molecules in enriched populations of mouse mammary stem cells and luminal cells that were isolated according to their differential expression of | 16849684 | [
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Steroid hormone receptor status of mouse mammary stem cells. | CD24 and the alpha6beta1-integrin complex. We found that the basal population; which is enriched in mouse mammary stem cells; did not express ERalpha; PR; or ErbB2/Her2 but did express epidermal growth factor receptor (EGFR)/ErbB1; whereas the subset of cells enriched for luminal cells expressed ERalpha (37% of cells) and PR (40% of cells) but not ErbB2/Her2 or EGFR/ErbB1. Ovariectomy confirmed the importance of estrogen signaling to luminal cell proliferation but had no effect on the size of the mouse mam | 16849684 | [
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Steroid hormone receptor status of mouse mammary stem cells. | mary stem cell-enriched population. Thus; mouse mammary stem cells were negative for ERalpha; PR; and ErbB2 and appeared to share common properties with poor-prognosis basal breast cancer. | 16849684 | [
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Inconsistent association between the STK15 F31I genetic polymorphism and breast cancer risk. | STK15 may be a low-penetrance breast cancer susceptibility gene; and several reports suggest that women who are homozygous for the polymorphic variant F31I have an increased risk of breast cancer. To evaluate this potential breast cancer allele; we genotyped 507 patients with two primary breast cancers and 875 population-based control subjects for the STK15 F31I polymorphism. All statistical tests were two-sided. The Ile/Ile homozygous genotype was not associated with an increased risk in white women of Bri | 16849685 | [
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Inconsistent association between the STK15 F31I genetic polymorphism and breast cancer risk. | tish descent. The odds ratio for developing two primary breast cancers) in Ile/Ile homozygotes was 0.63 (95% confidence interval [CI] = 0.34 to 1.13); which corresponds to an odds ratio of 0.79 (95% CI = 0.58 to 1.06) for a first primary breast cancer. A meta-analysis of this study and other published studies showed statistically significant heterogeneity in the odds ratio estimates (P<.001). This heterogeneity could reflect either population-specific linkage disequilibrium with a functional variant or arti | 16849685 | [
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Inconsistent association between the STK15 F31I genetic polymorphism and breast cancer risk. | facts such as population stratification or publication bias. | 16849685 | [
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Re: Diabetes mellitus and risk of colorectal cancer in the Singapore Chinese Health Study. | nan | 16849686 | [
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Re: Survival effects of postmastectomy adjuvant radiation therapy using biologically equivalent doses: a clinical perspective. | nan | 16849687 | [
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Re: Survival effects of postmastectomy adjuvant radiation therapy using biologically equivalent doses: a clinical perspective. | nan | 16849688 | [
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A modification to the flow method to estimate completeness in cancer registries with delayed registration. | For registries that routinely delay case registration; the flow method does not accurately describe their completeness over time. A modification to allow for such a delay is proposed and tested.Using original data set from Ticino Cancer Registry; Switzerland; a new data set (the delayed data set) was created by adding two years to the date of registration for each case in the original data set; to emulate the situation in a registry where registration is delayed by two years. Both the original and modified | 16849689 | [
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A modification to the flow method to estimate completeness in cancer registries with delayed registration. | methods were then applied to both the original and the delayed data set.When applied to the delayed data; the original method produced estimates of completeness of 32 and 43% at one and two years after diagnosis. When the modified method was applied to the delayed data; the completeness at one and two years was correctly estimated at 0%. After the initial two-year time lag; completeness was consistently estimated by both methods. When applied to the original data; the modified method produced the same resul | 16849689 | [
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A modification to the flow method to estimate completeness in cancer registries with delayed registration. | ts as the original method.The proposed modification allows the method to be applied even when registration is delayed long after diagnosis-thus extending the range of registries for which the flow method can be used. | 16849689 | [
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Caspase-mediated cleavage of ATM during cisplatin-induced tubular cell apoptosis: inactivation of its kinase activity toward p53. | Cisplatin induces renal cell injury and death; resulting in nephrotoxicity that limits its use in cancer therapy. Using cell culture models; recent work has suggested the involvement of p53 in renal cell apoptosis during cisplatin treatment. However; the signals upstream of p53 remain elusive. ATM and ATR are critical regulators of p53 under various conditions of DNA damage. Here; we show that ATM; and not ATR; was proteolytically cleaved into specific fragments of approximately 210 and 150 kDa during cispl | 16849690 | [
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Caspase-mediated cleavage of ATM during cisplatin-induced tubular cell apoptosis: inactivation of its kinase activity toward p53. | atin-induced tubular cell apoptosis. ATM cleavage was paralleled by the development of apoptosis. VAD; a broad-spectrum inhibitor of caspases; attenuated the cleavage of ATM; whereas the inhibitors of specific caspases were less effective. In caspase-3-deficient MCF-7 cells; ATM was cleaved; releasing the 210- but not the 150-kDa fragment. Recombinant caspase-3 was much more effective than caspase-7 in cleaving ATM that was immunoprecipitated from cell lysates. During cisplatin incubation; VAD protected ATM | 16849690 | [
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Caspase-mediated cleavage of ATM during cisplatin-induced tubular cell apoptosis: inactivation of its kinase activity toward p53. | and enhanced p53 phosphorylation. In vitro assay of protein kinase activity further showed that ATM immunoprecipitated from cisplatin-treated cells had significantly lower kinase activity toward p53 than that from control cells. Importantly; the protein kinase activity was restored in ATM that was protected by VAD during cisplatin incubation. ATM deficiency sensitized the cells to cisplatin-induced apoptosis; suggesting a cytoprotective role of ATM in this experimental model. Thus proteolysis of ATM by cas | 16849690 | [
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Caspase-mediated cleavage of ATM during cisplatin-induced tubular cell apoptosis: inactivation of its kinase activity toward p53. | pases may inactivate this regulatory molecule to facilitate the progression of apoptosis. | 16849690 | [
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Regulation of cation transport in the distal nephron by mechanical forces. | Thiazide and loop diuretics induce renal K(+) secretion; often leading to renal K(+) wasting and hypokalemia. This phenomenon has been proposed to reflect an increase in delivery to and reabsorption of Na(+) by the distal nephron; with a resultant increase in the driving force for passive K(+) efflux across the apical membrane. Recent studies suggest that cellular mechanisms that lead to enhanced rates of Na(+) reabsorption as well as K(+) secretion in response to increases tubular flow rates are more compl | 16849691 | [
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Regulation of cation transport in the distal nephron by mechanical forces. | ex. Increases in tubular flow rates directly enhance the activity of apical membrane Na(+) channels and indirectly activate a class of K(+) channels; referred to as maxi-K; that are functionally inactive under low flow states. This review addresses the role of biomechanical forces; generated by variations in urinary flow rate and tubular fluid volume; in the regulation of transepithelial Na(+) and K(+) transport in the distal nephron. The question of why the distal nephron has evolved to include a component | 16849691 | [
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Regulation of cation transport in the distal nephron by mechanical forces. | of flow-dependent K(+) secretion is also addressed. | 16849691 | [
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