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High urea and creatinine concentrations and urea transporter B in mammalian urinary tract tissues. | Although the mammalian urinary tract is generally held to be solely a transit and storage vehicle for urine made by the kidney; in vivo data suggest reabsorption of urea and other urine constituents across urinary tract epithelia. To determine whether urinary tract tissue concentrations are increased as a result of such reabsorption; we measured urea nitrogen and creatinine concentrations and determined whether urea transporter B (UT-B) was present in bladder; ureter; and other tissues from dogs and rats. M | 16849692 | [
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High urea and creatinine concentrations and urea transporter B in mammalian urinary tract tissues. | ean urea nitrogen and creatinine concentrations in dogs and rats were three- to sevenfold higher in urinary tract tissues than in serum and were comparable to those in renal cortex. In water-restricted or water-loaded rats; urea nitrogen concentrations in bladder tissues fell inversely with the state of hydration; were proportional to urine urea nitrogen concentrations; and were greater than the corresponding serum urea nitrogen concentration in every animal. Immunoblots of rat and dog urinary tract tissues | 16849692 | [
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High urea and creatinine concentrations and urea transporter B in mammalian urinary tract tissues. | demonstrated the presence of UT-B in homogenates of bladder and ureter; and immunocytochemical analysis localized UT-B to epithelial cell membranes. These findings are consistent with the notion that urea and creatinine are continuously reabsorbed from the urine across the urothelium; urea in part via UT-B; and that urine is thus altered in its passage through the urinary tract. Urea reabsorption across urinary tract epithelia may be important during conditions requiring nitrogen conservation and may contr | 16849692 | [
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Similitude of transperitoneal permeability in different rodent species. | Transgenic mice facilitate mechanistic studies of altered peritoneal transport; but the majority of transport studies have been carried out in rats. We hypothesized that mouse transport parameters; normalized to the peritoneal contact area; would be similar to those of the rat. To address this; we affixed small ( approximately 10-mm diameter) plastic chambers to the serosa of the abdominal wall of anesthetized CD1 and C57BL mice. The chamber constrained transfer across the area of the chamber base and facil | 16849694 | [
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Similitude of transperitoneal permeability in different rodent species. | itated mixing; volumetric; and concentration measurements vs. time for mannitol; serum albumin; and osmotic and hydrostatic pressure-driven convection. The mass transfer coefficient of mannitol (MTC(M)) and of serum albumin (MTC(BSA)); hydrostatic pressure-driven flux (J(P)); and osmotic filtration (J(osm)) were calculated from the time-dependent volume and concentration data. The units of all parameters (microl x min(-1) x cm(-2)) were compared with previously derived parameters from SD rats with a one-way | 16849694 | [
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Similitude of transperitoneal permeability in different rodent species. | ANOVA. Results indicated small but significant differences in MTC(BSA) (x10(2)): CD1; 9.72 +/- 1.97; n = 6; C57BL; 7.13 +/- 1.52; n = 10; rat; 12.5 +/- 1.6; n = 17 (P = 0.03). ANOVAs of all other parameters were not significant and confirmed our hypothesis: MTC(M) (CD1; 3.20 +/- 0.38; n = 7; C57BL; 2.34 +/- 0.41; n = 6; rat; 2.72 +/- 0.23 n = 19); J(P) (CD1; 0.77 +/- 0.15; n = 10; C57BL; 0.33 +/- 0.13; n = 15; rat; 0.51 +/- 0.16; n = 9); or J(osm) (CD1; 0.92 +/- 0.35; n = 6; C57BL; 0.49 +/- 0.35; n = 6; ra | 16849694 | [
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Similitude of transperitoneal permeability in different rodent species. | t 1.72 +/- 0.35; n = 6). We conclude that elimination of the variable peritoneal transfer area normalizes calculated transport characteristics and facilitates comparison between species. | 16849694 | [
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Myogenic vasoconstriction in mouse renal interlobar arteries: role of endogenous beta and gammaENaC. | Mechanosensitive ion channels are thought to initiate pressure-induced vasoconstriction; however; the molecular identity of these channels is unknown. Recent work from our laboratory suggests that members of the Degenerin/Epithelial Na+ Channel (DEG/ENaC) family may be components of the mechanosensitive ion channel complex in vascular smooth muscle (VSM); however; the specific DEG/ENaC proteins mediating myogenic constriction are unknown. The goal of this study is to determine if specific knockdown of beta | 16849693 | [
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Myogenic vasoconstriction in mouse renal interlobar arteries: role of endogenous beta and gammaENaC. | or gammaENaC; using dominant-negative (DN) or small-interference RNA (siRNA) molecules; inhibits pressure-induced vasoconstriction in mouse renal interlobar arteries. To address this goal; isolated arteries were transiently transfected with beta or gammaENaC DN-cDNA or siRNA molecules. After 24 h; vessels were either 1) cannulated and pressurized for pressure-diameter response curves or 2) dissociated and immunolabeled to determine VSM cell endogenous ENaC protein expression. We found that transfection of b | 16849693 | [
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Myogenic vasoconstriction in mouse renal interlobar arteries: role of endogenous beta and gammaENaC. | etaENaC DN-cDNA or siRNA suppresses beta-; but not gammaENaC protein expression. Similarly; gammaENaC DN-cDNA or siRNA suppresses gamma-; but not betaENaC protein expression. In addition; transfection of beta- or gammaENaC DN-cDNA or siRNA molecules inhibits pressure-induced vasoconstriction; but does not block agonist-induced vasoconstriction. Our results provide the first direct evidence that beta and gammaENaC proteins are essential in mediating myogenic vasoconstriction in mouse renal interlobar arterie | 16849693 | [
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Low Na intake suppresses expression of CYP2C23 and arachidonic acid-induced inhibition of ENaC. | We previously demonstrated that arachidonic acid (AA) inhibits epithelial Na channels (ENaC) through the cytochrome P-450 (CYP) epoxygenase-dependent pathway (34). In the present study; we tested the hypothesis that low Na intake suppresses the expression of CYP2C23; which is mainly responsible for converting AA to epoxyeicosatrienoic acid (EET) in the kidney (11) and attenuates the AA-induced inhibition of ENaC. Immunostaining showed that CYP2C23 is expressed in the Tamm-Horsfall protein (THP)-positive and | 16849695 | [
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Low Na intake suppresses expression of CYP2C23 and arachidonic acid-induced inhibition of ENaC. | aquaporin 2 (AQP2)-positive tubules. This suggests that CYP2C23 is expressed in the thick ascending limb (TAL) and collecting duct (CD). Na restriction significantly suppressed the expression of CYP2C23 in the TAL and CD. Western blot also demonstrated that the expression of CYP2C23 in renal cortex and outer medulla diminished in rats on Na-deficient diet (Na-D) but increased in those on high-Na diet (4%). Moreover; the content of 11;12-epoxyeicosatrienoic acid (EET) decreased in the isolated cortical CD f | 16849695 | [
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Low Na intake suppresses expression of CYP2C23 and arachidonic acid-induced inhibition of ENaC. | rom rats on Na-D compared with those on a normal-Na diet (0.5%). Patch-clamp study showed that application of 15 microM AA inhibited the activity of ENaC by 77% in the CCD of rats on a Na-D for 3 days. However; the inhibitory effect of AA on ENaC was significantly attenuated in rats on Na-D for 14 days. Furthermore; inhibition of CYP epoxygenase with MS-PPOH increased the ENaC activity in the CCD of rats on a control Na diet. We also used microperfusion technique to examine the effect of MS-PPOH on Na trans | 16849695 | [
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Low Na intake suppresses expression of CYP2C23 and arachidonic acid-induced inhibition of ENaC. | port in the distal nephron. Application of MS-PPOH significantly increased Na absorption in the distal nephron of control rats but had no significant effect on Na absorption in rats on Na-D for 14 days. We conclude that low Na intake downregulates the activity and expression of CYP2C23 and attenuates the inhibitory effect of AA on Na transport. | 16849695 | [
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Antagonism of endogenous putative P2Y receptors reduces the growth of MDCK-derived cysts cultured in vitro. | P2Y receptors couple to G proteins and either mobilize intracellular Ca(2+) or alter cAMP levels to modulate the activity of Ca(2+)- and cAMP-sensitive ion channels. We hypothesize that increased ion transport into the lumen of MDCK cysts can osmotically drive fluid movement and increase cyst size. Furthermore; activation of the adenylate cyclase/cAMP pathway may trigger cell proliferation via an extracellular signal-related kinase cascade. To test this hypothesis; several P2Y receptor inhibitors were used | 16849696 | [
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Antagonism of endogenous putative P2Y receptors reduces the growth of MDCK-derived cysts cultured in vitro. | on the MDCK in vitro model of renal cyst formation. The nonspecific P2 receptor inhibitors reactive blue 2 and suramin reduced cyst growth significantly; as did PPADS and; to a lesser extent; the P2Y(1)-specific antagonist MRS2179. Cyst growth was reduced by approximately 50% when ATP was removed from the culture medium with apyrase; although stable analogs of ATP failed to increase cyst size. The nonselective P2X receptor inhibitor Coomassie brilliant blue G was ineffective at reducing cyst growth; suggest | 16849696 | [
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Antagonism of endogenous putative P2Y receptors reduces the growth of MDCK-derived cysts cultured in vitro. | ing no involvement of P2X receptors. Finally; the presence of selective inhibitors of ERK activation (either PD98059 or U0126) greatly reduced cyst growth; whereas in untreated cysts ERK activity was observed to increase with time. We conclude that stimulation of endogenous P2Y receptors by extracellular ATP increases growth of MDCK cysts via cAMP-dependent activation of the ERK pathway. P2Y receptor antagonists may have therapeutic potential in reducing cyst size and slowing disease progression; although f | 16849696 | [
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Antagonism of endogenous putative P2Y receptors reduces the growth of MDCK-derived cysts cultured in vitro. | urther studies in vitro and in vivo are needed to investigate the specificity and role of these P2Y receptors in renal cystic diseases. | 16849696 | [
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Expression and interaction of two compound heterozygous distal renal tubular acidosis mutants of kidney anion exchanger 1 in epithelial cells. | Kidney AE1 (kAE1) is a glycoprotein responsible for the electroneutral exchange of chloride for bicarbonate; promoting the reabsorption of bicarbonate into the blood by alpha-intercalated cells of the collecting tubule. Mutations occurring in the gene encoding kAE1 can induce defects in urinary acidification resulting in distal renal tubular acidosis (dRTA). We expressed two kAE1 dRTA mutants; A858D; a mild dominant mutation; and DeltaV850; a recessive mutation; in epithelial Madin-Darby canine kidney (MDCK | 16849697 | [
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Expression and interaction of two compound heterozygous distal renal tubular acidosis mutants of kidney anion exchanger 1 in epithelial cells. | ) cells. Individuals heterozygous with wild-type (WT) kAE1 either did not display any symptoms of dRTA (DeltaV850/WT) or displayed a mild incomplete form of dRTA (A858D/WT); while compound heterozygotes (DeltaV850/A858D) had dRTA. We found that the A858D mutant was slightly impaired in the endoplasmic reticulum (ER) exit but could target to the basolateral membrane of polarized MDCK cells. Despite an altered binding to an inhibitor affinity resin; anion transport assays showed that the A858D mutant was func | 16849697 | [
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Expression and interaction of two compound heterozygous distal renal tubular acidosis mutants of kidney anion exchanger 1 in epithelial cells. | tional at the cell surface. The DeltaV850 mutant showed altered binding to the affinity resin but was predominantly retained in the ER; resulting in undetectable AE1 expression at the basolateral membrane. When coexpressed in MDCK cells; the WT protein; and to a lesser extent the A858D mutant; enhanced the cell surface expression of the DeltaV850 mutant. The DeltaV850 mutant also affected the cell surface expression of the A858D mutant. Compound heterozygous (A858D/DeltaV850) patients likely possess a decre | 16849697 | [
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Expression and interaction of two compound heterozygous distal renal tubular acidosis mutants of kidney anion exchanger 1 in epithelial cells. | ased amount of functional anion exchangers at the basolateral membrane of their alpha-intercalated cells; resulting in impaired bicarbonate transport into the blood and defective acid transport into the urine. | 16849697 | [
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MicroRNAs from the Planarian Schmidtea mediterranea: a model system for stem cell biology. | MicroRNAs (miRNAs) are approximately 22-nt RNA molecules that typically bind to the 3' untranslated regions of target mRNAs and function to either induce mRNA degradation or repress translation. miRNAs have been shown to play important roles in the function of stem cells and cell lineage decisions in a variety of organisms; including humans. Planarians are bilaterally symmetric metazoans that have the unique ability to completely regenerate lost tissues or organs. This regenerative capacity is facilitated b | 16849698 | [
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MicroRNAs from the Planarian Schmidtea mediterranea: a model system for stem cell biology. | y a population of stem cells known as neoblasts. Planarians are therefore an excellent model system for studying many aspects of stem cell biology. Here we report the cloning and initial characterization of 71 miRNAs from the planarian Schmidtea mediterranea. While several of the S. mediterranea miRNAs are members of miRNA families identified in other species; we also identified a number of planarian-specific miRNAs. This work lays the foundation for functional studies aimed at addressing the role of these | 16849698 | [
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Tysabri's return draws cautious optimism. Pharmacies, infusion centers must register to participate. | nan | 16849700 | [
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Timing, duration of surgical antimicrobial prophylaxis need attention. | nan | 16849702 | [
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Precision and accuracy of two blood glucose meters: FreeStyle Flash versus One Touch Ultra. | The precision and accuracy of two blood glucose meters were evaluated using finger and forearm blood samples.Duplicate blood glucose measurements on the same forearm and finger as venipuncture were performed with the FreeStyle Flash and the One Touch Ultra. Accuracy was assessed by error-grid analysis and the number of values within 10% of the laboratory reference value. Precision was determined by calculating the absolute mean percent differences in glucose values between the first and second fingers and f | 16849704 | [
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Precision and accuracy of two blood glucose meters: FreeStyle Flash versus One Touch Ultra. | orearm test results. Forearm testing success was defined as an accurate glucose reading obtained with one lance.A total of 100 patients completed the study; 93% had diabetes and 53% were female. Patients' mean +/- S.D. age was 63 +/- 12 years; and glucose measurements ranged from 69 to 354 mg/dL. All finger-stick samples fell within error-grid zones A and B; 72% and 57% of FreeStyle Flash and One Touch Ultra values fell within 10% of the laboratory reference values; respectively (p = 0.027). Forearm samples | 16849704 | [
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Precision and accuracy of two blood glucose meters: FreeStyle Flash versus One Touch Ultra. | were successfully obtained in 99 and 74 patients using the FreeStyle Flash and One Touch Ultra (p < 0.001); with 64 and 36 samples; respectively; falling within 10% of the laboratory reference values (p = 0.035). There was no difference in meter precision.The FreeStyle Flash and the One Touch Ultra are precise glucose meters; however; the FreeStyle Flash was associated with greater accuracy. Success rates of forearm glucose sampling were significantly greater when the FreeStyle Flash meter was used. | 16849704 | [
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Effect of ingesting cranberry juice on bacterial growth in urine. | nan | 16849705 | [
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Development and implementation of group medical visits at a family medicine center. | nan | 16849711 | [
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Control of pneumococcal disease in the United Kingdom--the start of a new era. | In 2000; a multi-valent pneumococcal conjugate vaccine; known as Prevnar; was licensed for use in infants and young children in the USA. The subsequent introduction of the vaccine into the childhood immunization schedule in that country led to a significant decrease in pneumococcal disease. The vaccine is effective against invasive and non-invasive pneumococcal infection; can be used in young children as well as adults and; like all conjugate vaccines; provides long-lasting immunity. Moreover; it reduces th | 16849715 | [
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Control of pneumococcal disease in the United Kingdom--the start of a new era. | e incidence of antibiotic resistance because a number of resistant serotypes are targeted by the vaccine. Prevnar; also known as Prevenar; has since been licensed in numerous countries; including the UK. On 8 February 2006; the Departments of Health in England; Scotland and Wales announced the inclusion of Prevenar in the childhood immunization schedule. This announcement has important implications for pneumococcal infection; disease surveillance and immunization policy in the UK. | 16849715 | [
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Endocarditis caused by Propionibacterium species: a report of three cases and a review of clinical features and diagnostic difficulties. | Propionibacterium species are members of the normal flora of skin and the mouth but their pathogenic potential is often overlooked. Three fatal cases of endocarditis caused by Propionibacterium species over an 8-year period are reported; and a review is presented of a further 33 cases from the world literature. In most cases; infection was protracted; with minimal signs in the early stages. Fourteen cases (42.4%) involved native valves; 16 (48.5%) involved prosthetic valves and three (9.1%) were associated | 16849716 | [
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Endocarditis caused by Propionibacterium species: a report of three cases and a review of clinical features and diagnostic difficulties. | with other intracardiac prosthetic material. Intracardiac abscesses were commonly encountered; with Propionibacterium endocarditis occurring in 28.6% of native valve infections and 52.9% of prosthetic valve infections. A very high proportion of all of the cases (70.6%) required surgical intervention. Several factors appeared to delay institution of appropriate therapy and may have contributed to abscess formation; including an indolent clinical course; negative or delayed culture results; and the tendency t | 16849716 | [
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Endocarditis caused by Propionibacterium species: a report of three cases and a review of clinical features and diagnostic difficulties. | o consider this organism as a blood-culture contaminant. The authors recommend careful clinical evaluation before disregarding a blood-culture isolate of Propionibacterium spp. as a skin contaminant; and consideration of this bacterium as a potential cause of apparently culture-negative endocarditis. | 16849716 | [
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Increased recovery of Moraxella catarrhalis and Haemophilus influenzae in association with group A beta-haemolytic streptococci in healthy children and those with pharyngo-tonsillitis. | The inflamed tonsils harbour numerous types of bacteria; alone or in combination with group A beta-haemolytic streptococci (GABHS). The cohabitation of the tonsils by GABHS and certain other bacterial species may contribute to the inflammatory process and the failure of penicillin therapy. This study evaluated the recovery of Moraxella catarrhalis; Haemophilus influenzae; Staphylococcus aureus and Streptococcus pneumoniae in association with GABHS in healthy children and those with acute pharyngo-tonsilliti | 16849717 | [
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Increased recovery of Moraxella catarrhalis and Haemophilus influenzae in association with group A beta-haemolytic streptococci in healthy children and those with pharyngo-tonsillitis. | s (APT). Pharyngo-tonsillar cultures were obtained from 548 children with APT and 866 healthy children. GABHS was recovered from 112 (20.4%) children with APT. Of the 114 H. influenzae isolates; 32 were recovered in association with GABHS (29% of all patients who had GABHS) and 82 were isolated without GABHS (19%) (P=0.0267). Of the 69 M. catarrhalis isolates; 25 were recovered in association with GABHS (22% of all patients who had GABHS) and 44 were isolated without GABHS (10%) (P=0.0012). In contrast; the | 16849717 | [
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Increased recovery of Moraxella catarrhalis and Haemophilus influenzae in association with group A beta-haemolytic streptococci in healthy children and those with pharyngo-tonsillitis. | re was no association between the isolation of GABHS and the recovery of Staph. aureus or Strep. pneumoniae. GABHS was recovered from 104 (12%) healthy children. Of the 69 M. catarrhalis isolates; 24 were recovered in association with GABHS (23% of all patients who had GABHS) and 80 were isolated without GABHS (10%) (P=0.006). There was no association between the isolation of GABHS and the recovery of H. influenzae; Staph. aureus or Strep. pneumoniae. This study demonstrates an association between the recov | 16849717 | [
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Increased recovery of Moraxella catarrhalis and Haemophilus influenzae in association with group A beta-haemolytic streptococci in healthy children and those with pharyngo-tonsillitis. | ery of GABHS and H. influenzae and M. catarrhalis from pharyngo-tonsillar cultures of patients with APT and M. catarrhalis from pharyngo-tonsillar cultures of healthy children. | 16849717 | [
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Distribution of 19 major virulence genes in Legionella pneumophila serogroup 1 isolates from patients and water in Queensland, Australia. | The distribution of 19 major virulence genes and the presence of plasmids were surveyed in 141 Legionella pneumophila serogroup (SG) 1 isolates from patients and water in Queensland; Australia. The results showed that 16 of the virulence genes examined were present in all isolates; suggesting that they are life-essential genes for isolates in the environment and host cells. The 65 kb pathogenicity island identified originally in strain Philadelphia-1(T) was detected more frequently in isolates from water (4 | 16849718 | [
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Distribution of 19 major virulence genes in Legionella pneumophila serogroup 1 isolates from patients and water in Queensland, Australia. | 4.2%) than in those from patients (2.7%); indicating that the 65 kb DNA fragment may aid the survival of L. pneumophila in the sampled environment. However; the low frequency of the 65 kb fragment in isolates from patients suggests that the pathogenicity island may not be necessary for L. pneumophila to cause disease. Plasmids were not detected in the L. pneumophila SG1 isolates from patients or water studied. There was an association of both lvh and rtxA with the virulent and predominant genotype detected | 16849718 | [
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Distribution of 19 major virulence genes in Legionella pneumophila serogroup 1 isolates from patients and water in Queensland, Australia. | by amplified fragment length polymorphism; termed AF1; whereas the avirulent common isolate from water termed AF16 did not have lvh or rtxA genes; with the exception of one isolate with rtxA. It was found that a PCR detection test strategy with lvh and rtxA as pathogenesis markers would be useful for determining the infection potential of an isolate. | 16849718 | [
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Biofilm matrix of Candida albicans and Candida tropicalis: chemical composition and role in drug resistance. | Matrix material was extracted from biofilms of Candida albicans and Candida tropicalis and analysed chemically. Both preparations contained carbohydrate; protein; hexosamine; phosphorus and uronic acid. However; the major component in C. albicans matrix was glucose (32%); whereas in C. tropicalis matrix it was hexosamine (27%). Biofilms of C. albicans were more easily detached from plastic surfaces by treatment with the enzyme lyticase (beta-1;3-glucanase) than were those of C. tropicalis. Biofilms of C. al | 16849719 | [
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Biofilm matrix of Candida albicans and Candida tropicalis: chemical composition and role in drug resistance. | bicans were also partially detached by treatment with proteinase K; chitinase; DNase I; or beta-N-acetylglucosaminidase; whereas C. tropicalis biofilms were only affected by lipase type VII or chitinase. To investigate a possible role for the matrix in biofilm resistance to antifungal agents; biofilms of C. albicans were grown under conditions of continuous flow in a modified Robbins device (MRD). These biofilms produced more matrix material than those grown statically; and were significantly more resistant | 16849719 | [
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Biofilm matrix of Candida albicans and Candida tropicalis: chemical composition and role in drug resistance. | to amphotericin B. Biofilms of C. tropicalis synthesized large amounts of matrix material even when grown statically; and such biofilms were completely resistant to both amphotericin B and fluconazole. Mixed-species biofilms of C. albicans and a slime-producing strain of Staphylococcus epidermidis (RP62A); when grown statically or in the MRD; were also completely resistant to amphotericin B and fluconazole. Mixed-species biofilms of C. albicans and a slime-negative mutant of S. epidermidis (M7); on the oth | 16849719 | [
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Biofilm matrix of Candida albicans and Candida tropicalis: chemical composition and role in drug resistance. | er hand; were completely drug resistant only when grown under flow conditions. These results demonstrate that the matrix can make a significant contribution to drug resistance in Candida biofilms; especially under conditions similar to those found in catheter infections in vivo; and that the composition of the matrix material is an important determinant in resistance. | 16849719 | [
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Gene-expression profiles in gastric epithelial cells stimulated with spiral and coccoid Helicobacter pylori. | Human gastric epithelial immortalized GES-1 cells were infected with spiral and coccoid Helicobacter pylori. Scanning electron microscopy was used to determine the ability of the two forms of H. pylori to adhere to GES-1 cells. GES-1 cell apoptosis induced by coccoid and spiral H. pylori was analysed using flow cytometry. A cDNA microarray for 22;000 human genes was used to identify the gene-expression differences in GES-1 cells infected with the two forms of H. pylori; and the gene expression identified by | 16849720 | [
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Gene-expression profiles in gastric epithelial cells stimulated with spiral and coccoid Helicobacter pylori. | the cDNA microarray was confirmed by RT-PCR. Scanning electron microscope observation showed that both coccoid and spiral bacteria can adhere to GES-1 cells. After 4 h infection; apoptosis induction was 27.4% for spiral-form infection and 10.2% for coccoid-form infection. Of 268 differentially expressed genes identified by cDNA microarray; 166 showed higher expression with the spiral H. pylori infection than with the coccoid H. pylori infection. To the best of the authors' knowledge; this is the first repo | 16849720 | [
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Gene-expression profiles in gastric epithelial cells stimulated with spiral and coccoid Helicobacter pylori. | rt that GES-1 cells infected with spiral H. pylori have higher expression of cxcl10; ccl11; ccl5; groalpha; TLR5; ATF3; fos; fosl2; gadd45a and myc. The cells infected with coccoid H. pylori had higher expression of survivin. The global profile of gene expression in GES-1 cells infected with coccoid and spiral H. pylori is described for the first time. | 16849720 | [
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Increased prostanoid dependency of arterial relaxation in Chlamydia pneumoniae-infected mice. | Endothelial dysfunction plays an important role in the development of atherosclerosis. Previous studies have shown that inoculation with Chlamydia pneumoniae contributes to atherosclerotic development in rabbits and hypercholesterolaemic mice and causes endothelial dysfunction in apolipoprotein E-deficient mice. The effect of acute C. pneumoniae infection on endothelial function in normocholesterolaemic C57BL/6J mice was studied by measuring the force of contraction of the descending aorta after noradrenali | 16849721 | [
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Increased prostanoid dependency of arterial relaxation in Chlamydia pneumoniae-infected mice. | ne stimulation and in response to methacholine-induced relaxation. In addition; the effects of the nitric oxide synthase inhibitor N(omega)-nitro-L-arginine methyl ester (L-NAME) and the cyclooxygenase inhibitor diclofenac on relaxation were assessed. Pre-treatment of the aortas with L-NAME decreased the relaxation response in both the infected and uninfected groups and no significant difference was detected between these groups; whereas diclofenac significantly attenuated the relaxation response only in th | 16849721 | [
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Increased prostanoid dependency of arterial relaxation in Chlamydia pneumoniae-infected mice. | e infected animals. In conclusion; infection shifted the balance of endothelium-derived relaxing factors from nitric oxide towards vasorelaxing prostanoids in C57BL/6J mice. | 16849721 | [
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A microarray analysis of the murine macrophage response to infection with Francisella tularensis LVS. | The response of cells of the mouse macrophage cell line J774 to infection with Francisella tularensis LVS was analysed by means of a DNA microarray representing approximately 18;500 genes (20;600 clones). The adaptive response was modest at all time points; and at most; 81 clones were differentially regulated from the time point of uptake of bacteria (0 min) up to 240 min later. For all five time points; 229 clones fulfilled the criteria of being differentially regulated; i.e. the ratio between infected ver | 16849722 | [
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A microarray analysis of the murine macrophage response to infection with Francisella tularensis LVS. | sus non-infected cells was at least 1.7-fold up- or down-regulated and P <0.05. It was found that many of the differentially regulated genes are known to respond to stress in general and to oxidative stress specifically. However; at 120 min it was observed that genes that lead to depletion of glutathione were upregulated. Possibly; this was a result of mechanisms induced by F. tularensis. Generally; there was a conspicuous lack of inflammatory responses and; for example; although tumour necrosis factor alph | 16849722 | [
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A microarray analysis of the murine macrophage response to infection with Francisella tularensis LVS. | a (TNF-alpha) was upregulated at 0 min; a significant down-regulation was noted at all subsequent time points. When cells were treated with an inhibitor of inducible nitric oxide synthase (iNOS) or the antioxidant N-acetylcysteine (NAC); the infection-induced cytopathogenic effect was significantly inhibited. Together; the results suggest that F. tularensis LVS infection confers an oxidative stress upon the target cells and that many of the host-defence mechanisms appear to be intended to counteract this st | 16849722 | [
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Molecular typing of enteroviruses associated with viral meningitis in Cyprus, 2000-2002. | Human enteroviruses are responsible for a wide spectrum of clinical diseases affecting many different organ systems. Although infection is usually asymptomatic; infections of the central nervous system manifested as meningitis or encephalitis can pose a serious public health problem; especially during outbreaks. In this study; samples from 218 patients diagnosed with enteroviral meningitis between January 2000 and December 2002 were analysed in order to assess the epidemiology of human enteroviruses as a ca | 16849723 | [
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Molecular typing of enteroviruses associated with viral meningitis in Cyprus, 2000-2002. | use of viral meningitis in Cyprus. A new typing strategy; based on partial sequencing of the 5' non-coding region (5'NCR); prediction of type; and selection of type-specific primers for sensitive VP1 PCR amplification; was developed. As clustering in the 5'NCR was concordant with clustering in the VP1 region; quick and reliable typing by VP1 sequencing was achieved without virus isolation in cell culture. The most frequent enterovirus serotypes identified were Human echovirus 30 (55.5%); Human echovirus 13 | 16849723 | [
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Molecular typing of enteroviruses associated with viral meningitis in Cyprus, 2000-2002. | (15.1%); Human echovirus 6 (13.8%) and Human echovirus 9 (8.3%). Human coxsackieviruses B2; B1 and B5; Human echovirus 4; Human enterovirus 71 and Human coxsackievirus A6 represented rather rare serotypes. This is the first molecular epidemiological study of enterovirus meningitis in Cyprus. Serotype distribution corresponded basically with observations in other European countries; suggesting the spread of enteroviruses by tourism. | 16849723 | [
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Bordetella holmesii DNA is not detected in nasopharyngeal swabs from Finnish and Dutch patients with suspected pertussis. | Bordetella holmesii is a Gram-negative bacterium first identified in 1995. It can cause pertussis-like symptoms in humans. B. holmesii contains insertion sequences IS481 and IS1001; two frequently used targets in the PCR diagnosis of Bordetella pertussis and Bordetella parapertussis infections. To investigate the prevalence of B. holmesii in Finnish and Dutch patients with pertussis-like symptoms and whether B. holmesii has caused any false-positive results in diagnostic PCRs; B. holmesii-specific real-time | 16849724 | [
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Bordetella holmesii DNA is not detected in nasopharyngeal swabs from Finnish and Dutch patients with suspected pertussis. | PCRs were developed. The Finnish methods were conventional IS481 PCR and B. holmesii-specific real-time PCR (LightCycler; Roche) targeting the B. holmesii recA gene. The Dutch methods were IS481 and IS1001 PCRs with conventional or real-time formats and B. holmesii-specific real-time PCR targeting the homologue of IS1001. Of 11;319 nasopharyngeal swabs; 2804 were collected from Finnish patients from 2000 to 2003; and 8515 from Dutch patients from 1992 to 2003. B. holmesii DNA was not found in the samples a | 16849724 | [
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Bordetella holmesii DNA is not detected in nasopharyngeal swabs from Finnish and Dutch patients with suspected pertussis. | nalysed. The results suggest that B. holmesii is not among the causative agents of pertussis-like symptoms in Finnish and Dutch patients and thus does not in practice confound IS481 and IS1001 PCRs. | 16849724 | [
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Genotyping of Campylobacter jejuni using seven single-nucleotide polymorphisms in combination with flaA short variable region sequencing. | This investigation describes the development of a generally applicable; bioinformatics-driven; single-nucleotide polymorphism (SNP) genotyping assay for the common bacterial gastrointestinal pathogen Campylobacter jejuni. SNPs were identified in silico using the program 'Minimum SNPs'; which selects for polymorphisms providing the greatest resolution of bacterial populations based on Simpson's index of diversity (D). The high-D SNPs identified in this study were derived from the combined C. jejuni/Campyloba | 16849726 | [
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Genotyping of Campylobacter jejuni using seven single-nucleotide polymorphisms in combination with flaA short variable region sequencing. | cter coli multilocus sequence typing (MLST) database. Seven SNPs were found that provided a D of 0.98 compared with full MLST characterization; based on 959 sequence types (STs). The seven high-D SNPs were interrogated using allele-specific real-time PCR (AS kinetic PCR); which negates the need for expensive labelled primers or probes and requires minimal assay optimization. The total turnaround time of the SNP typing assay was approximately 2 h. Concurrently; 69 C. jejuni isolates were subjected to MLST an | 16849726 | [
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Genotyping of Campylobacter jejuni using seven single-nucleotide polymorphisms in combination with flaA short variable region sequencing. | d flagellin A short variable region (flaA SVR) sequencing and combined with a population of 84 C. jejuni and C. coli isolates previously characterized by these methods. Within this collection of 153 isolates; 19 flaA SVR types (D=0.857) were identified; compared with 40 different STs (D=0.939). When MLST and flaA SVR sequencing were used in combination; the discriminatory power was increased to 0.959. In comparison; SNP typing of the 153 isolates alone provided a D of 0.920 and was unable to resolve a small | 16849726 | [
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Genotyping of Campylobacter jejuni using seven single-nucleotide polymorphisms in combination with flaA short variable region sequencing. | number of unrelated isolates. However; addition of the flaA SVR locus to the SNP typing procedure increased the resolving power to 0.952 and clustered isolates similarly to MLST/flaA SVR. This investigation has shown that a seven-member C. jejuni SNP typing assay; used in combination with sequencing of the flaA SVR; efficiently discriminates C. jejuni isolates. | 16849726 | [
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Rapid cost-effective subtyping of meticillin-resistant Staphylococcus aureus by denaturing HPLC. | The importance of meticillin-resistant Staphylococcus aureus (MRSA) in hospital-acquired infection is widely acknowledged. The UK government has stated that MRSA bloodstream infection rates will have to be halved by 2008. Such radical improvements will require advances on several fronts. Screening for MRSA in high-risk patients on arrival at hospital allows isolation of carriers and reduces transmission to staff and other patients. Concurrent subtyping of MRSA could also inform outbreak investigations and l | 16849725 | [
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Rapid cost-effective subtyping of meticillin-resistant Staphylococcus aureus by denaturing HPLC. | ong-term epidemiological studies. The variability within the staphylococcal protein A; or spaA; gene-repeat region can be used as a marker of short- and long-term genetic variation. A novel application is described of denaturing HPLC (DHPLC) for rapid; inexpensive characterization of spaA gene amplification products; without the need for DNA sequence determination. The method allowed rapid and precise sizing of spaA gene-repeat regions from 99 S. aureus strains and was combined with heteroduplex analysis; u | 16849725 | [
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Rapid cost-effective subtyping of meticillin-resistant Staphylococcus aureus by denaturing HPLC. | sing reference PCR products; to indicate the spa type of the test isolate. The method allowed subtyping of strains in less than 5 h from receipt of a primary isolation plate. When applied to an outbreak that occurred during this study; the authors were able to demonstrate relatedness of the isolates more than 5 days before results were received from a reference laboratory. If combined with direct amplification from swabs; DHPLC analysis of spaA gene variation could prove extremely valuable in outbreak inves | 16849725 | [
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Identification of a repetitive sequence belonging to a PPE gene of Mycobacterium tuberculosis and its use in diagnosis of tuberculosis. | A repetitive sequence specific to Mycobacterium tuberculosis was isolated from a lambda gt11 library of M. tuberculosis by DNA-DNA hybridization using genomic DNA of M. tuberculosis as probe followed by subtractive hybridization with a cocktail of other mycobacterial DNA. This led to identification of CD192; a 1291 bp fragment of M. tuberculosis containing repetitive sequences; which produced positive hybridization signals with M. tuberculosis DNA within 30 min. Nucleotide sequencing revealed the presence o | 16849727 | [
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Identification of a repetitive sequence belonging to a PPE gene of Mycobacterium tuberculosis and its use in diagnosis of tuberculosis. | f several direct and inverted repeats within the 1291 bp fragment that belonged to a PPE family gene (Rv0355) of M. tuberculosis. The use of CD192 as a DNA probe for the identification of M. tuberculosis in culture and clinical samples was investigated. The 1291 bp sequence was present in M. tuberculosis; Mycobacterium bovis and M. bovis BCG; but was not present in many of the other mycobacterial strains tested; including M. tuberculosis H37Ra. More than 300 clinical isolates of M. tuberculosis were probed | 16849727 | [
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] |
Identification of a repetitive sequence belonging to a PPE gene of Mycobacterium tuberculosis and its use in diagnosis of tuberculosis. | with CD192; and the presence of the 1291 bp sequence was observed in all the clinical strains; including those lacking IS6110. The sequence displayed RFLP among the clinical isolates. A PCR assay was developed which detected M. tuberculosis with 100% specificity from specimens of sputum; cerebrospinal fluid and pleural effusion from clinically diagnosed cases of tuberculosis. | 16849727 | [
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Pneumococci causing invasive disease in children prior to the introduction of pneumococcal conjugate vaccine in Scotland. | This study aimed to determine the serotypes and sequence types (STs) of pneumococci causing paediatric invasive disease in Scotland prior to the introduction of pneumococcal conjugate vaccines (PCVs). All invasive pneumococci isolated between 2000 and 2004 from children aged less than 5 years in Scotland were used. The isolates were characterized by serotyping and multi-locus sequence typing. Two hundred and seventeen pneumococci were characterized into 22 different serogroups/types; the most common; in ran | 16849728 | [
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Pneumococci causing invasive disease in children prior to the introduction of pneumococcal conjugate vaccine in Scotland. | k order; being 14; 19F; 6B; 18C; 23F; 9V; 4; 1; 19A and 6A. They were further genotyped into 77 different STs; the three most common being 9; 162 and 176. Common serotypes possessed multiple STs; but pneumococci of a particular clone were mostly associated with a particular serotype. The seven most common serotypes are included in the 7-valent polysaccharide conjugate vaccine (PCV7). Serotype coverage for PCV7 was 76.5% in those aged less than 5 years but increased to 88.9% for those aged 1 year. The introd | 16849728 | [
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Pneumococci causing invasive disease in children prior to the introduction of pneumococcal conjugate vaccine in Scotland. | uction of PCV7 into the childhood immunization schedule would reduce the burden of pneumococcal disease in children; although continued surveillance of invasive pneumococcal disease will be required before; during and after the introduction of PCVs. | 16849728 | [
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Development of a diagnostic test for the Midlands 1 cystic fibrosis epidemic strain of Pseudomonas aeruginosa. | In a previous study of isolates from cystic fibrosis (CF) patients in England and Wales; the Midlands 1 strain of Pseudomonas aeruginosa was identified as the second most common clone; representing 10% of isolates and found in nearly one-third of all CF centres [Scott; F. W. & Pitt; T. L. (2004). J Med Microbiol 53; 609-615]. Using suppression subtractive hybridization; 54 sequences were identified as present in a Midlands 1 strain but were absent from strain PAO1. The distribution of 14 of these sequences | 16849729 | [
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Development of a diagnostic test for the Midlands 1 cystic fibrosis epidemic strain of Pseudomonas aeruginosa. | amongst representatives of Midlands 1; other CF epidemic strains and unrelated P. aeruginosa CF isolates was determined using PCR assays. Using these data; a PCR-based test was developed that was specific for the Midlands 1 clone; which was confirmed using dot-blot hybridization. By applying the test to CF isolates from a CF centre in Liverpool; a Midlands 1 clone was identified. The identity was confirmed using typing by PFGE. The PCR test should facilitate a greater understanding of the distribution of th | 16849729 | [
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Development of a diagnostic test for the Midlands 1 cystic fibrosis epidemic strain of Pseudomonas aeruginosa. | e Midlands 1 strain in the UK and elsewhere. | 16849729 | [
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Role of the ABC transporter TruMDR2 in terbinafine, 4-nitroquinoline N-oxide and ethidium bromide susceptibility in Trichophyton rubrum. | A single-copy gene; designated TruMDR2; encoding an ATP-binding cassette (ABC) transporter was cloned and sequenced from the dermatophyte Trichophyton rubrum. The ORF of TruMDR2 was 4048 nt and the deduced amino acid sequence showed high homology with ABC transporters involved in drug efflux in other fungi. The encoded ABC protein predicted 12 transmembrane segments (TMSs) and two almost identical nucleotide-binding domains (NBDs) arranged in two halves in a (TMS(6)-NBD)(2) configuration and could be classi | 16849730 | [
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Role of the ABC transporter TruMDR2 in terbinafine, 4-nitroquinoline N-oxide and ethidium bromide susceptibility in Trichophyton rubrum. | fied as a member of the multidrug-resistance (MDR) class of ABC transporters. Northern blot analyses revealed an increased level of transcription of the TruMDR2 gene when mycelium was exposed to acriflavine; benomyl; ethidium bromide; ketoconazole; chloramphenicol; griseofulvin; fluconazole; imazalil; itraconazole; methotrexate; 4-nitroquinoline N-oxide (4NQO) or tioconazole. Disruption of the TruMDR2 gene rendered the mutant more sensitive to terbinafine; 4NQO and ethidium bromide than the control strain; | 16849730 | [
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Role of the ABC transporter TruMDR2 in terbinafine, 4-nitroquinoline N-oxide and ethidium bromide susceptibility in Trichophyton rubrum. | suggesting that this transporter plays a role in modulating drug susceptibility in T. rubrum. | 16849730 | [
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Epidemiological analysis of group A streptococci recovered from patients in China. | Since the mid-1980s; there has been a resurgence of severe forms of invasive group A streptococcal (GAS) disease in many countries and regions. However; there has not been any systemic epidemiologic analysis of GAS disease reported in mainland China. To analyse the molecular epidemiology of GAS disease; 86 strains from patients in different regions of mainland China were collected. The collection sites included blood; pus; wounds; the epipharynx and other sites. A total of 21 different emm types were identi | 16849731 | [
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Epidemiological analysis of group A streptococci recovered from patients in China. | fied in the isolates. In both invasive and non-invasive isolates; M1 (29.1%) and M12 (23.3%) were the most prevalent types; a different distribution to M type distributions reported in other countries. Furthermore; minor emm gene sequence alterations were noted for six types. Several important GAS virulence factors were detected by PCR using specific primers. The speB and slo genes were detected in all isolates and were species specific. Four superantigen genes; speA; speC; smeZ and ssa; were found in 52% ( | 16849731 | [
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Epidemiological analysis of group A streptococci recovered from patients in China. | 45/86); 51% (44/86); 82% (71/86) and 23% (27/86) of isolates; respectively. M1 isolates harboured more speA (84%) and fewer speC genes (44%); while M12 isolates had fewer speA (35%) and more speC genes (100%). There was also an association between some virulence genes and isolation sites; perhaps due to the correlation between the emm type distribution and virulence gene occurrence. For two important virulence genes related to necrotizing fasciitis; the sil gene was only carried by 11 of 86 isolates; and no | 16849731 | [
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Epidemiological analysis of group A streptococci recovered from patients in China. | sil gene contained the start codon ATA. The sla gene rarely occurred in GAS isolates; only four of 86 GAS strains being positive; including two isolates obtained from blood. In antimicrobial susceptibility tests; the overall rate of drug resistance in GAS isolates was higher than reported rates in other countries; and the resistance rates to erythromycin; tetracycline and clindamycin were 91.8; 93.4 and 80%; respectively. This epidemiological study may help to understand the pathogenesis of GAS disease and | 16849731 | [
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Epidemiological analysis of group A streptococci recovered from patients in China. | aid in vaccine development. | 16849731 | [
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