nopperl/pmc-image-text · Datasets at Hugging Face

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0.40849	301526bf4d1d4d4caab73e4b6392f330	Images for troubleshooting(A) Images of the intermediate lobe (IL) remaining in the anterior lobe side (AL).(B) Images of neuron-like sharp cells in primary culture. The enlarged image is shown in the lower panel.(C) Failure of aggregate formation using low purity adult pituitary stem/progenitor cells. Scale bars: 1 mm (A) and 100 μm (B and C).	PMC9168163	gr8.jpg
0.420738	abdfa40d8af24603921432121ae4551c	A, Clinical image of 5.5-cm × 5.0-cm brown patch with variegated pigmentation involving the right zygomatic area with presence of scar postbiopsy. B, Dermatoscopic image of the scar, showing mild perifollicular hyperpigmentation near the lateral border of the scar.	PMC9168378	gr1.jpg
0.450778	cf2fb96ee39b4d7eb2700428bd0e46dc	A, Reflectance confocal microscopy image (0.75 mm × 0.75 mm, Vivascope 3000; Caliber Imaging and Diagnostics, Inc); location: epidermis. Presence of a predominantly honeycomb pattern and atypical dendritic-shaped pagetoid cells aggregating near follicles (arrows), typical for lentigo maligna. B, The dermoepidermal junction, showing widespread disarray with the presence of dendritic cells (arrows) adjacent to follicles in the background of an unspecified pattern (asterisk).	PMC9168378	gr2.jpg
0.454467	73b847eeeff0445093bb69db675754bb	Area of concern identified with handheld RCM stained with a small drop of surgical dye (arrow) to serve as a landmark for pathologists. Surrounding marks were made using a sterile marker to delineate the exterior position of the confocal probe’s plastic ending, with central area ink stain correlated with the direct central position of cellular atypia on RCM. RCM, Reflectance confocal microscopy.	PMC9168378	gr3.jpg
0.468694	33d97565bddb4806ae821e4ab4877a43	Histologic image showing junctional proliferations of enlarged, atypical melanocytes with hyperchromatic and pleomorphic nuclei arranged in heterogenous nests (stars). Green surgical dye used for landmarking noted along superficial epidermis.	PMC9168378	gr4.jpg
0.399403	f2d2e1d91d6e40df8b403d0a30510a61	Participant selection flow chart. RESCUE-RE refers to a registration study for Critical Care of Acute Ischemic Stroke After Recanalization. mRS: modified Rankin Scale.	PMC9168462	fneur-13-877773-g0001.jpg
0.460222	9f4c5c69a44342e18f86a92f0ab521ce	The mRS score at 3 months. mRS, modified Rankin Scale.	PMC9168462	fneur-13-877773-g0002.jpg
0.445535	ae382e2790e44fc3820b7e3b3aed596a	Subgroup analysis of the primary outcome. The forest plot shows the differences in odds ratio for primary outcome (modified Rankin Scale [mRS], 0–2) at 90 days in subgroups. Adjusted for age, sex, diabetes mellitus, hypertension, atrial fibrillation, dyslipidemia, smoking, education, insurance, time from stroke onset to admission interval, mRS before the stroke, baseline NIHSS score, NIHSS score 24h after EVT, the side of the brain where the stroke occurred, stroke cause, TICI score of 2b or 3. OR, odds ratio; CI, confidence interval; mRS, modified Rankin Scale; TICI, thrombolysis in cerebral infarction; NIHSS, National Institute of Health Stroke Scale.	PMC9168462	fneur-13-877773-g0003.jpg
0.409124	7eba018fd4aa4f7eb2bd48e9da7650fa	Patient selection, exclusion, and inclusion criteria. PMF, posterior malleolus fracture.	PMC9168892	10.1177_10711007211070540-fig1.jpg
0.430648	2c229590c0a84928abd9173a1d752c24	Histograms of the distribution of Self-Reported Foot and Ankle Score (SEFAS) in patients treated within (upper panel) and after (lower panel) a week from injury.	PMC9168892	10.1177_10711007211070540-fig2.jpg
0.505475	42af7c2f09664767814e96ae110b0e54	Diagnosis and treatment strategies for the neonatal outpatient service and emergency room during the coronavirus disease 2019 pandemic. COVID-19: Coronavirus disease 2019. Ma LS, Zhao YL, Wei YD, Liu C. Recommendations for perinatal and neonatal surgical management during the COVID-19 pandemic. World Journal of Clinical Cases. 2020;8(14):2893–901. This figure is from a previous publication by our team	PMC9170880	383_2022_5136_Fig1_HTML.jpg
0.543159	3a818c75937a49128ba53e693f60ca3e	Transportation strategies for severe and critically ill patients from the neonatal surgery department during the coronavirus disease 2019 pandemic. COVID-19: Coronavirus disease 2019; SARS-CoV-2: Severe acute respiratory syndrome coronavirus 2. Ma LS, Zhao YL, Wei YD, Liu C. Recommendations for perinatal and neonatal surgical management during the COVID-19 pandemic. World Journal of Clinical Cases. 2020;8(14):2893–901. This figure is from a previous publication by our team	PMC9170880	383_2022_5136_Fig2_HTML.jpg
0.433649	c009c900ba654c91aca43a1af17aa0ac	Neonatal emergency surgery procedures in the neonatal surgery department during the coronavirus disease 2019 pandemic. COVID-19: Coronavirus disease 2019; CT: Computed tomography. Ma LS, Zhao YL, Wei YD, Liu C. Recommendations for perinatal and neonatal surgical management during the COVID-19 pandemic. World Journal of Clinical Cases. 2020;8(14):2893–901. This figure is from a previous article by our team	PMC9170880	383_2022_5136_Fig3_HTML.jpg
0.38921	35e451f579d64db0b19f05691130cc92	Study flow diagram. CDH congenital diaphragmatic hernia; NTG Normal time group; STG Special time group	PMC9170880	383_2022_5136_Fig4_HTML.jpg
0.42008	9f153f29bc7a451593875ef278073f12	Overall photo of case 1, a 2.5-year-old girl.	PMC9171574	aqab204f0001.jpg
0.419952	87cf6eb398b54305bf455d600f39fe5f	Overall photo of case 2, a 4-year-old girl.	PMC9171574	aqab204f0002.jpg
0.475149	1ba50dd5da954886b6db38b4226f44f9	Overall photo of case 3, a 10-year-old girl.	PMC9171574	aqab204f0003.jpg
0.51496	0ca4a501152e4cf0889681d8f5c3c48e	Scalp of case 1.	PMC9171574	aqab204f0004.jpg
0.480457	a0ceb581a84f4ac8af088052b37e2ded	Thymic involution of each case (A, C, E), with thymus of age-matched controls (B, D, F). (Glass microscope slides stained with H&E, all photographed with ×2.5 camera macro lens.)	PMC9171574	aqab204f0005.jpg
0.537102	d528576bca8b4aafaec2cbd2c897897d	Low-power views depicting myocardium with diffuse hypereosinophilia in 3 cases (H&E, ×10 objectives). A, Case 1; B, case 2; C, case 3.	PMC9171574	aqab204f0006.jpg
0.430963	cd0f9ae29bb84d43b29629b5983f2abc	Higher-power photomicrographs depicting myocyte catecholamine necrosis in 3 cases, including contraction banding, hypereosinophilia, myofibrillar disarray and dissolution, cytoplasmic vacuolization, and interstitial edema. A, Case 1; B, C, D, case 2; E, F, case 3. Cases 2 and 3 also demonstrate focal myocyte hypertrophy. (H&E; A, B, ×40 objectives; C, D, E, F, ×60 objectives).	PMC9171574	aqab204f0007.jpg
0.456	ce528dd50d1a4317b8231574bc0eaa81	Scalp of case 2.	PMC9171574	aqab204f0008.jpg
0.451572	1ed4eeba29e14be5a2270fb6a6865b41	Anitschkow-like chromatin patterns in myocardium from case 2 (A, B, C) and case 3 (D, E, F). A-E, Longitudinal sections display “caterpillar” pattern of nuclear chromatin, appearing to be of the myocytes. Slightly tangential sections of nuclei are in interstitial cells (arrows). F, Two transverse sections of nuclei display the “owl eye” pattern (arrows). (H&E, original objective magnifications ×60)	PMC9171574	aqab204f0009.jpg
0.440418	1bd24284cc84453c971d0a6c67576c46	Scalp of case 3.	PMC9171574	aqab204f0010.jpg
0.421	3eb2fc198a644d42a2423abed6039c4b	Overall photo of consult case 3.75-year-old girl.	PMC9171574	aqab204f0011.jpg
0.480971	df40dbb72b5049a69b791d2a74ba76f6	Scalp of consult case.	PMC9171574	aqab204f0012.jpg
0.478118	6c909ca82511460da702b35d3a9bba1c	Thymus of older patients depicting normal age-related involution. A, Well-preserved distinction between cortex and medulla despite overall decrease of parenchyma. B, Interstitium with fatty infiltration of septa. (Glass microscope slides stained with H&E, both with ×3.0 camera macro lens)	PMC9171574	aqab204f0013.jpg
0.43555	cf4cfe7dc0534d9c89f082464ffc0ef4	Animals and information included in the genetic prediction scenarios. a Shows the relationship between purebred and crossbred pigs, as well as the number of phenotypes/genotypes collected for purebred and crossbred parents and offspring. b Shows the source of phenotypic information used for the genomic prediction analysis for each phenotyping scenario, with the diagram showing an example where crossbred offspring of group-4 are the animals to be predicted. c Shows the sources of genomic information for each genotyping scenario. The figure was created with BioRender.com	PMC9171933	12711_2022_736_Fig1_HTML.jpg
0.420689	cbead453d4394daeaed77136a32bf5b7	Estimates of genetic correlations between crossbred and purebred traits. The plot shows the strength and direction of the genetic correlations between purebred and crossbred traits. Darker colors indicate a stronger correlation, while red and purple indicate a positive and negative correlation, respectively	PMC9171933	12711_2022_736_Fig2_HTML.jpg
0.427156	1774c384647945558ca47bdfcc56cf23	Estimates of genetic correlations between the crossbred traits in the breeding objective in selection index for scenarios CB-Live, CB-FOM, and CB-Color. The plot shows the strength and direction of the genetic correlations between the breeding objectives and crossbred traits in the selection criteria. Darker colors indicate a stronger	PMC9171933	12711_2022_736_Fig3_HTML.jpg
0.424498	9dc899454f81406a818a870d786af47a	First two principal components for variation in the genomic relationship matrix	PMC9171933	12711_2022_736_Fig4_HTML.jpg
0.45605	6089cc811c2841f3808c1df8e2606142	Accuracy of (genomic) prediction using various genotyping strategies in the crossbred phenotyping scenarios. Each panel represents a breeding objective. The x-axis shows the phenotyping scenario (ST/CB-Live/CB-FOM/CB-Color/PB-1/PB-2/PB-3), and the y-axis shows the accuracy obtained. Genotyping scenarios are represented by shape/color combinations: black circle (no genotyping), brown triangle (Stage-1), red square (Stage-2), and purple line (Stage-3). For the definitions of the phenotyping scenarios, please refer to the “Indirect selection for carcass traits using purebred growth phenotypes” and “Less expensive crossbred phenotypes for the selection of meat quality” sections. For the definitions of the genotyping scenarios, please refer to the “Prediction of crossbred traits” section	PMC9171933	12711_2022_736_Fig5_HTML.jpg
0.456933	2b2260bba3a9450884359c7379658b61	Accuracy of (genomic) prediction using various genotyping strategies in the purebred phenotyping scenarios. Each panel represents a breeding objective. The x-axis shows the phenotyping scenario (ST/PB-1/PB-2/PB-3), and the y-axis shows the accuracy obtained. Genotyping strategies are represented by shape/color combinations: black circle (no genotyping), brown triangle (Stage-1), red square (Stage-2), and purple line (Stage-3). For the definitions of the phenotyping scenarios, please refer to the “Indirect selection for carcass traits using purebred growth phenotypes” and “Less expensive crossbred phenotypes for the selection of meat quality” sections. For the definitions of the genotyping scenarios, please refer to the “Prediction of crossbred traits” section	PMC9171933	12711_2022_736_Fig6_HTML.jpg
0.438863	2d4fb076814146f7a4f17a42448cfffa	CFS + LM suspension to correct unilateral congenital blepharoptosis. a: Preoperative image of a five-year-old boy with congenital blepharoptosis OD; b: incision line labeled with methylene blue; c: exposure of CFS and LM; d: image of the patient twenty-two months after CFS + LM suspension	PMC9175472	12886_2022_2469_Fig1_HTML.jpg
0.418778	6b0c2d20fb6541db9bc8fec1ec1fa719	Microscopic images of the CFS sections. (Victoria Blue staining, × 10)	PMC9175472	12886_2022_2469_Fig2_HTML.jpg
0.378095	7807f4534cfd4d4ea35af043d54c5084	Results of immunofluorescent staining of the CFS and LM in the three age groups. (NC: negative contrast)	PMC9175472	12886_2022_2469_Fig3_HTML.jpg
0.451826	d455febb1dc64461828b23d5528c9742	Western blot showing elastin/β-actin expression in the CFS and LM in the three age groups	PMC9175472	12886_2022_2469_Fig4_HTML.jpg
0.561385	0aceac952118453bbfe55d0aaf325395	Elastin expression in the CFS and LM of all groups. A: child group; B: adolescent group; C: adult group; D: elastin expression of CFS in the child, adolescent and adult groups. (* P < 0.05, ** P < 0.01)	PMC9175472	12886_2022_2469_Fig5_HTML.jpg
0.458724	efd41f8b96a54bceaad3a5ad2788442c	Western blot of elastin expression in the CFS in the child, adolescent and adult groups	PMC9175472	12886_2022_2469_Fig6_HTML.jpg
0.42478	db53749c9f2e4ce0b3cf634610ae0d11	Structure of RBM.	PMC9177386	fpsyg-13-843427-g001.jpg
0.480934	e6efc82230974d34bc483cb995de92cc	Comparison between time-domain waveform after pre-emphasis of pipa music segment and the original time-domain waveform.	PMC9177386	fpsyg-13-843427-g002.jpg
0.454426	a183c073cc8a41bfa3ab27aa3bd3bd2b	Framing diagram of the pipa music clip.	PMC9177386	fpsyg-13-843427-g003.jpg
0.482973	c30145bd21aa4da584f117811e34857f	The standard MFCC parameter extraction process for traditional instrumental music.	PMC9177386	fpsyg-13-843427-g004.jpg
0.507499	b14b9742393c4e0cabd8aaaa14225fa4	The first-dimensional MFCC characteristic parameters of the guzheng music segment.	PMC9177386	fpsyg-13-843427-g005.jpg
0.480051	6899851e276443908cf7178277347a6e	The DBN-based structure of the national musical instrument recognition and differentiation network.	PMC9177386	fpsyg-13-843427-g006.jpg
0.432956	b8986d3cefe54b5d803efe1369fcbe2c	The retrieval framework of differentiated music library platform.	PMC9177386	fpsyg-13-843427-g007.jpg
0.469515	bafbfb603ca8408db4733a8b4f4bbdef	Comparison of prediction accuracy of different hidden layers for national musical instruments.	PMC9177386	fpsyg-13-843427-g008.jpg
0.456178	d85ae7fcb7784533b3fdb959c3b1d5f9	The average accuracy of the discrimination algorithm based on the DBN and traditional discriminator for national musical instrument recognition.	PMC9177386	fpsyg-13-843427-g009.jpg
0.453617	03395e05d36141388c06cf9ecaead624	The confusion matrix composed of recognition rates [(A) recognition rate confusion matrix of distinguishing national musical instruments by distinguishing trees; (B) recognition rate confusion matrix of distinguishing national musical instruments by KNN].	PMC9177386	fpsyg-13-843427-g010.jpg
0.465217	987f7850345b4329854fc4e17c0cc207	The confusion matrix of recognition accuracy [(A) the recognition rate confusion matrix of SVM for distinguishing the national musical instruments; (B) the recognition rate confusion matrix of softmax for distinguishing the national musical instruments].	PMC9177386	fpsyg-13-843427-g011.jpg
0.414318	dac59b52cf8d4fe8a2f8307c3f682e1e	The confusion matrix of recognition accuracy of the DBN and softmax in distinguishing the national musical instruments.	PMC9177386	fpsyg-13-843427-g012.jpg
0.419265	976f737aac21498ab40f34eecf1794aa	(A) Phenotypes of the Williams 82, Gmpgl3-1, and Gmpgl3-2 mutants. Scale bars, 5 cm. (B–G) Pigment contents and photosynthesis parameters of the Williams 82, Gmpgl3-1, and Gmpgl3-2 mutants. (B) Chlorophyll a (Chl a), chlorophyll b (Chl b) and carotenoid (Car). (C) Chlorophyll fluorescence parameter (Fv/FM). (D) Photosynthetic rate (Pn). (E) Transpiration rate (Tr). (F) Stomatal conductance (Gs). (G) Intercellular CO2 concentration (Ci). (H) Chloroplast structure in Williams 82. (I,J) Chloroplast structure in the Gmpgl3-1 and Gmpgl3-2 mutants. Scale bars, 1 μm. *** represents significant differences compared with the control (Williams 82) at p < 0.001, and the error bars represent standard deviations.	PMC9178232	fpls-13-892077-g001.jpg
0.484644	080afd73bdec44b7a7d25a8bcc7d494a	Map-based cloning of the GmTic110a locus. (A) SNP index plot of all chromosomes of the F2 plants. (B) SNP index plots of chromosome 02 of the Gmpgl3a mutant from the F2 population. (C) Physical position of the GmTic110a candidate gene. (D) Schematic diagram showing the structure of GmTic110a. The red lines indicate mutation sites within the GmTic110a gene in the two mutant lines.	PMC9178232	fpls-13-892077-g002.jpg
0.416064	2a1fb6170a5f4a9ebf9c094731384284	(A) Multiple sequence alignments of the Tic110 protein in Arabidopsis thaliana (At), Glycine max (Gm), Physcomitrella patens (Pp), Chlamydomonas reinhardtii (Cr), and Pisum sativum (Ps). The dark region represents identical amino acids, and the grey region represents similar amino acids. (B) Schematic diagram representations of the two structural models of the Tic110 protein. In terms of the locations of the proposed TM domains, the red boxes represent TM1 and TM2, the orange boxes represent TM3 to TM6, the blue boxes represent transit peptides (TPs), the green box represents TP binding, and the yellow box represents a co-domain. (C) Phylogenetic trees based on the multiple sequence alignments of the Tic110 proteins. Bootstrap values from 1,000 replicates are indicated at each node. (D) Conserved motifs of Tic110 proteins in G. max, A. thaliana, Medicago sativa, Oryza sativa, Zea mays, Sorghum bicolor, Selaginella tamariscina, and P. patens were identified using the MEME search tool. Different motifs (1–12) are represented by boxes with different colors. (E) Tissue-specific expression profiles were determined via qRT–PCR.	PMC9178232	fpls-13-892077-g003.jpg
0.479946	42c40989bad1462b80e9fd16ca16b902	(A) Phenotypes of Williams 82, the Gmpgl3 mutant, and CRISPR/Cas9-edited plants (GmTic110aCR1, GmTic110aCR,2 and GmTic110aCR3). Scale bar = 1 cm. (B) sgRNA target sequences of Williams 82, GmTic110aCR1, GmTic110aCR2, and GmTic110aCR3. The sgRNA target sequence is shown in blue letters, and the protospacer-adjacent motif (PAM) site is shown in yellow letters. The red letters indicate a single-base substitution. – indicates a deletion of the corresponding nucleotide. (C) Relative expression of the GmTic110a gene in unifoliate leaves of Williams 82, the Gmpgl3 mutant, and CRISPR/Cas9-edited plants. The asterisks indicate statistically significant differences, as determined by Student’s t-test (***, p < 0.001), and the error bars represent the standard deviations.	PMC9178232	fpls-13-892077-g004.jpg
0.445248	8166af78f1b94120b97e2ecc9f8c9296	Subcellular localization of GmTic110a, GmTic110aG114A, Gmtic110aT805S, GmTic110aCR1, GmTic110aCR2, and GmTic110aCR3. (A-G) Transient expression of GFP-GmTic110a, GFP-GmTic110aG114A, GFP-Gmtic110aT805S, GFP-GmTic110aCR1, GFP-GmTic110aCR2, GFP-GmTic110aCR3 and GFP in Arabidopsis protoplasts. GFP, GFP fluorescence; Chlorophyll, chlorophyll autofluorescence; Bright, bright field. Merged, merged image of GFP fluorescence, chlorophyll autofluorescence and bright field images. Scale bars = 10 μm.	PMC9178232	fpls-13-892077-g005.jpg
0.430199	2a56002866d848368cebded623855ef6	GmTic110a interacts with GmTic20, GmTic40a, and GmTic40b. (A,C,E) Luciferase complementation assay showing that GmTic110a interacts with GmTic20, GmTic40a, and GmTic40b in Nicotiana benthamiana. Luciferase activity was detected 3 days after injection. (B,D,F) Interactions with GmTic20, GmTic40a, and GmTic40b in N. benthamiana according to a Co-IP assay. Immunoblots of the total protein extracts (20% input) and the immunoprecipitation product were performed using an anti-HA antibody (a-HA) or an anti-FLAG antibody (a-FLAG), respectively.	PMC9178232	fpls-13-892077-g006.jpg
0.39569	540f3664852245ef9049c83d4cbb9809	Distribution diagrams of tea plants used in this study and results of paternity assignments. a EW plantation for seed collection and the tea cultivars surrounding it. b EW offspring planting in the Mingshan experimental field. c ASR distribution for 14 candidate fathers. d Distribution of ssASR of each offspring with the 14 hypothetical parental couples. e Distribution of trio LOD scores of the 392 offspring when set with EW × CM as a parental couple. f Venn diagram showing the common results provided by the three paternity assignment methods for the EW × CM couple.	PMC9178331	uhac086f1.jpg
0.434741	4a6bf56c55354750aa6712aabbee44ce	Results of population structure analyses of the samples tested in this study. a Population structure obtained from admixture when K = 5 and 10. b Scatter plot based on the first two principal components (PCs) of the principal component analysis.	PMC9178331	uhac086f2.jpg
0.419939	1b4f39408e0a4d91bc83a402c3aed1bf	High-density linkage map constructed using genotypic data of the 217 full-sib offspring of EW × CM. a Distribution of SNP markers on the 15 linkage groups. b Collinearity analysis of the genetic positions of markers on the map and physical positions in the reference genome. c Heat maps showing distances and recombination rates between markers. The rates of recombination increase from yellow to purple. Gray indicates no available value for the recombination rate between the two markers.	PMC9178331	uhac086f3.jpg
0.38882	464bdd91d0ec4744bcc3e2ab7e8179e4	Phenotypic variation of SPI among the offspring in two consecutive years. a Representative pictures of tea buds with different SPIs. The SPI value is given in each picture. b and c Distribution of the 388 EW offspring based on SPI_2020 and SPI_2021. d and e Comparisons of average SPI values in different crosses. The significances of difference between two groups are indicated by P values from Kruskal–Wallis tests.	PMC9178331	uhac086f4.jpg
0.413981	213142ea713542cf9cef699c4cf47ee6	QTL mapping results with the SPI trait in the EW × CM full-sib population. a LOD value for 2020 and 2021 covering the whole genome by interval mapping. b–d MQM mapping results in Chr3 and Chr4. Genome-wide LOD thresholds at 99 and 95% for QTL claiming are shown by dotted lines. Cofactors used for MQM mapping are indicated by arrows in b–d.	PMC9178331	uhac086f5.jpg
0.389057	9d87c125dd404b379d63c8287717fce7	Characteristics of wecB::Cm mutant of S. Gallinarum. (A) Growth curves of wild-type and wecB::Cm mutant in Luria-Bertani (LB) broth. The bacteria grown in LB broth for 14 h were diluted to OD600 = 1.5 and inoculated 1/100 into the LB broth and cultured at 37°C with shaking (150 rpm). The optical density (OD600) was measured at 0, 2, 4, 6, 8, 10, 12, and 24 h after inoculation. (B) Bacterial counts of wild-type and wecB::Cm mutant in LB broth. The bacteria were cultivated, as described above. At the indicated time points, 100 μl of each culture was serially diluted in LB broth and 100 μl of each dilution was spread on an LB agar plate. After incubating overnight at 37°C, count the colonies on the plate as colony forming units (CFU). (C–E) Bacterial growth characteristics of wecB::Cm mutant in LB broth containing bile acid (C), hydrogen peroxide (D), or nalidixic acid (E). The bacteria were grown in LB broth for 14 h and then diluted to 2.0 ×107 CFU/ml. The diluted bacterial culture (50 μl) was mixed with 50 μl of each reagen in 96-well flat-bottom plates and incubated at 37°C without shaking. The optical density (OD595) was measured at 0, 2, 4, 6, 8, 10, and 24 h after inoculation. The data are means ± standard deviations based on six wells per group at each time point. The significant differece was shown as **p < 0.01.	PMC9178343	fmicb-13-880932-g0001.jpg
0.431465	51d1707d433748efa349209330131a56	Pathological finding in the chickens orally infected with wild-type or wecB::Cm mutant strains. (A) Survival curve of chickens orally inoculated with wild-type or wecB::Cm mutant. Chickens were orally inoculated with 108 CFU of wild-type or wecB::Cm mutant, and survival of the chickens was recorded for 14 days post-infection. (B) Gross lesions in the liver of chickens orally inoculated with wild-type or wecB::Cm mutant. The livers of pathological changes were observed on 3, 5, and 7 days post-infection (dpi). White lesions and small necrotic foci were observed in wild-type infected chickens on 3 days after infection, and liver hypertrophy and congestion were observed on 7 days after infection. In the chickens infected with wecB::Cm mutant, although slight white lesions and small necrotic foci were observed on 5 days after infection, no hypertrophy or swelling was observed.	PMC9178343	fmicb-13-880932-g0002.jpg
0.598283	62084556484145ea97ce25e3faee3729	Viable bacterial counts in the liver and spleen of chickens orally inoculated with wild-type or wecB::Cm mutant. Chickens were orally inoculated with 108 CFU of wild-type or wecB::Cm mutant. The bacteria in the liver and spleen were determined on 1, 3, 5, and 7 days post-infection. The data are means ± standard deviations based on three to six chickens per group at each time point. The significant differences were shown as **p < 0.01.	PMC9178343	fmicb-13-880932-g0003.jpg
0.412989	9405357809494af297f0911228031689	Histopathological changes and microscopic lesions in the livers of chickens orally infected with wild-type or wecB::Cm mutant strain. Chickens were orally inoculated with 108 CFU of wild-type or wecB::Cm mutant and the livers were collected on 3, 5, and 7 days post-infection. The organs of uninfected chickens were used as the controls. Paraffin sections of the organs were prepared and stained with hematoxylin-eosin (HE). (A) Magnification 100 × and (B) magnification 400 × . Arrows show lesions which were characterized by marked infiltration of heterophils and lymphocytes with degeneration and necrosis.	PMC9178343	fmicb-13-880932-g0004.jpg
0.490589	3c304d09c77c4aeb959dbd8991aa7b46	Histopathological changes and microscopic lesions in the spleens of chickens orally infected with wild-type or wecB::Cm mutant strain. Chickens were orally inoculated with 108 CFU of wild-type or wecB::Cm mutant and the livers were collected on 3, 5, and 7 days post-infection. The organs of uninfected chickens were used as the controls. Paraffin sections of the organs were prepared and stained with HE. (A) Magnification 100 × and (B) magnification 400 × . Arrows show degeneration and necrosis in white pulp.	PMC9178343	fmicb-13-880932-g0005.jpg
0.457589	1ac5cc2cc1554e36b62e1103e999a6e1	Expression of cytokine and chemokine in the liver (A) and spleen (B) of chickens infected with wild-type or wecB::Cm mutant strains. Chickens were inoculated orally with 108 CFU of wild-type or wecB::Cm mutant. The liver and spleen of the chickens were collected on 3 and 5 days post-infection, and the expression of interleukin-1β, IL-6, tumor necrosis factor-α, interferon-γ, IL-12, and CXCLi1 was determined by quantitative RT-PCR. Data were expressed as means ± standard deviations of fold changes in gene expression of the organs from infected groups relative to those from the uninfected control group (three chickens per group at each time point). Statistical analysis was performed using one-way ANOVA analysis followed by Tukey's multiple comparison test to compare infected chickens with uninfected controls. The significant differences were shown as p < 0.05, *p < 0.01.	PMC9178343	fmicb-13-880932-g0006.jpg
0.414154	9a24c29def9a48d69efb837543f1b695	Kinetics of bacterial colonization and protective immune responses in the chickens orally infected with wecB::Cm mutant. (A) Viable bacterial numbers in the liver and spleen of chickens infected with wild-type or wecB::Cm mutant strains. Chickens were orally inoculated with 108 CFU of wild-type or wecB::Cm mutant, and the number of bacteria in the organs was determined on 1, 3, 5, 7, 11, 20, 25, 35, and 45 days post-infection. The data are means ± standard deviations based on three to six chickens per group at each time point. (B) Antibody production in chickens infected with wecB::Cm mutant strain. Five Chickens were orally inoculated with 108 CFU of wecB::Cm mutant, and the anti-S. gallinarum antibodies were determined by serum agglutination test on 45 days after infection. PBS and serum from uninfected chickens (n = 5) were used as a negative control, and anti-O9 serum was used as a positive control. (C) Survival curve of chickens orally inoculated with wecB::Cm mutant and re-challenged with wild-type strain on 35 days after initial inoculation. wecB::Cm-inoculated chickens were re-challenged with 108 CFU of the wild-type strain, and the survival of chickens was recorded for 14 days after re-infection with the wild-type strain.	PMC9178343	fmicb-13-880932-g0007.jpg
0.422678	3a1b85894690493f81c266f0a9fd6f42	Anti-spike and anti-RBD titers after primary vaccination and boosting in individuals with PAD(A and B) Anti-spike (A) and RBD (B) (ancestral strain) endpoint titers in 48 lots of 6 different immunoglobulin replacement products (squares) compared with 20 HD (blue circles) before and 14 and 90 days after completion of the BNT162b2 vaccine series.(C and D) Anti-spike (C) and RBD (D) endpoint titers in HDs (n = 20; blue circles), COVID-19-naive individuals with PAD (n = 18, red circles), and COVID-19-experienced individuals with PAD (n = 9, green circles) before or 14 and 90 days after completion of a mRNA vaccination series (BNT162b2, n = 19; mRNA-1273, n = 8).(E and F) Anti-spike (E) and RBD (F) endpoint titers in COVID-19-naive individuals with PAD (n = 16, red circles) and COVID-19-experienced individuals with PAD (n = 3, green circles) before (n = 6) and 14 or 28 (n = 19), 90 (n = 18), and 150 (n = 12) days after completion of a primary mRNA (BNT162b2, n = 14; mRNA-1273, n = 3) or Ad26.COV2.S (n = 2) vaccine series and 14 (n = 19) days and 90 (n = 13) days after booster with a mRNA vaccine (BNT162b2, n = 16; mRNA-1273, n = 3). A dotted black line represents the limit of detection (1/50).(G and H) Anti-spike avidity index in HDs (n = 19, blue circles), COVID-19-naive individuals with PAD (red circles), and COVID-19-experienced individuals with PAD (green circles) 14 and 90 days after primary vaccination (n = 16, COVID-19-naive; n = 6, COVID-19-experienced), 150 days after primary vaccination (n = 10, COVID-19-naive; n = 2, COVID-19-experienced), 14 days after boosting (n = 12, COVID-19-naive; n = 3, COVID-19-experienced), and 90 days after boosting (n = 10, COVID-19-naive; n = 1, COVID-19-experienced).Numbers above graphed data (C–F) represent the geometric mean titer (GMT) for each time point and average avidity index (G and H). Bars indicate median (A and B) values. Kruskal-Wallis with Dunn’s post-test (A–F), paired t test (G), and one-way ANOVA with Dunnett’s post-test (H). Only significant differences are shown: ∗p < 0.05, ∗∗p < 0.01, ∗∗∗p < 0.001, ∗∗∗∗p < 0.0001. See also Figure S1 and Tables S1–S3.	PMC9179023	gr1.jpg
0.389805	d90b826706314d14bcbcfcefe27a73d0	IgG subclasses and FcγR-binding activity of anti-spike antibodies in serum from vaccinated individuals with PAD(A–J) Levels of IgG1(A), IgG2 (B), IgG3 (C), IgG4 (D), IgA (E), IgM (F), FcγR2A-binding (G), FcγR2B-binding (H), FcγR3A-binding (I), and FcγR3B-binding (J) ancestral (S, S1, S2, and RBD), B.1.351, and B.1.617.2 spike antibodies in HDs (n = 20, blue circles), COVID-19-naive individuals with PAD (n = 18, red circles), and COVID-19-experienced individuals with PAD (n = 9, green circles) 14 days after completion of the second dose of the mRNA vaccine series. Two-way ANOVA with Tukey post-test, mean. Only significant differences are shown: ∗p < 0.05, ∗∗p < 0.01, ∗∗∗p < 0.001, ∗∗∗∗p < 0.0001). See also Figure S2 and Table S3.	PMC9179023	gr2.jpg
0.480591	c967b4381004465b84307a86f0b1b520	Neutralizing antibody responses in individuals with PAD after vaccination and boosting(A and B) Serum neutralizing activity against WA1/2020 (A) or B.1.617.2 (Delta) (B) in COVID-19-naive (red circles) and COVID-19-experienced (green circles) individuals with PAD before (n = 4, COVID-19-naive; n = 5, COVID-19-experienced) and 14 (n = 18, COVID-19-naive; n = 9, COVID-19-experienced) or 90 (n = 17, COVID-19-naive; n = 6, COVID-19-experienced) days after mRNA vaccination and 14 days after mRNA booster (n = 14, COVID-19-naive; n = 3, COVID-19-experienced). Shown is the neutralizing activity of immunoglobulin replacement products (n = 17, purple squares).(C and D) Effect of boosting on serum neutralization of WA1/2020 (C) and B.1.617.2 (D) in COVID-naive (n = 16, red circles) and COVID-19-experienced (n = 3, green circles) individuals with PAD after completion of the primary mRNA (14 or 90 days after vaccination; BNT162b2, n = 14; mRNA-1273, n = 3) or Ad26.COV2.S (28 or 90 days after vaccination, n = 2) vaccine series and 14 (n = 19) or 90 (n = 13) days after boosting with a mRNA vaccine (BNT162b2, n = 16; mRNA-1273, n = 3).(E–G) Effect of variant strains on serum neutralizing activity of individuals with PAD 14 days after completion of mRNA vaccination (E) (n = 27 total; n = 18, COVID-19-naive, red circles; n = 9, COVID-19-experienced, green circles), 14 days after boosting (F) (n = 19 total; n = 16, COVID-19-naive, red circles; n = 3, COVID-19-experienced, green circles), and 90 days after boosting (G) (n = 13 total; n = 12 COVID-19-naive, red circles; n = 1, COVID-19-experienced, green circle).LOD, limit of detection. A dotted black line represents the presumptive protective titer as described.28 Numbers immediately above the x axis indicate the number and percentage of individuals with serum-neutralizing titers above 50 at each time point. Numbers above graphed data represent the GMT for each time point. Kruskal-Wallis with Dunn’s post-test. Only significant differences are shown: ∗p < 0.05; ∗∗p < 0.01; ∗∗∗p < 0.001; ∗∗∗∗p < 0.0001). See also Figure S3 and Table S3.	PMC9179023	gr3.jpg
0.409194	106e2def98984f10ae421861c30ecf47	Primed and naïve hESC analysis and XIST expression. (A) Schematic representation of the pipeline of the analysis of expression of X-linked genes and their allelic expression. (B) XIST expression by TPM (transcripts per million) values in primed (blue) and naïve (red) hESCs across 9 different studies. The whiskers were calculated by the default ggplot code in R (upper whisker: min(max(x)), Q3 + 1.5 × IQR, lower whisker: max(min(x)), Q1 − 1.5 × IQR, where Q3 and Q1 are the third and first quartiles, and IQR is Q3 − Q1). The dots represent the outliers. (C) Distribution of XIST status changes across all naïve samples.	PMC9179337	cells-11-01729-g001.jpg
0.393926	2c76225e2cfb44f389722165697df30c	Changes in gene and allelic expression in chromosome X. (A) Average TPM expression of genes in chromosome X of primed (blue) and naïve (red) hESCs across 9 different studies. Whiskers and dots were calculated as mentioned in the legend to Figure 1. (B) Distribution of X-linked gene expression variations between naïve samples and their primed counterparts. (C) Average TPM expression of genes on chromosome X of primed (blue) and naïve (red) hESCs. p-value was calculated by a paired t-test. (D) Average X:autosomes allelic ratio of primed and naïve cells. p-value was calculated by a paired t-test. (E) Distribution of allelic expression variation across all naïve samples.	PMC9179337	cells-11-01729-g002.jpg
0.474278	cef59072112043b2a3fc51eaffe8372b	Geographical distribution of gene and allelic expression along chromosome X. (A) Moving average plot of median log2 gene expression naïve/primed ratio along chromosome X, separated into XIST+ (brown) and XIST− (green) samples. The blue line marks the location of the centromere, and the red line marks the location of XIST. The dashed lines define Xq22. (B) Chromosome X gene expression differences between XIST+ and XIST− samples in the entire X chromosome and in Xq22 area. p-value was calculated by a paired t-test. (C) X:autosomes allelic ratio in the entire chromosome X (light blue) versus Xq22 region (pink) in XIST+ samples. p-value was calculated by a paired t-test.	PMC9179337	cells-11-01729-g003.jpg
0.43849	d84f541138d348cdb45711e2e665c368	PRISMA flow diagram of the study selection process. From: Page, M.J.; McKenzie, J.E.; Bossuyt, P.M.; Boutron, I.; Hoffmann, T.C.; Mulrow, C.D.; Shamseer, L.; Tetzlaff, J.M.; Akl, E.A.; Brennan, S.E.; et al. The PRISMA 2020 statement: an updated guideline for reporting systematic reviews. BMJ 2021, 372, n71. doi: 10.1136/bmj.n71 [17]. For more information, visit: http://www.prisma-statement.org/ (accessed on 1 April 2022).	PMC9179989	ijerph-19-06378-g001.jpg
0.436217	54efa25940de48589d7161aa9bfdbeae	Jingning county ferry distribution map.	PMC9180115	ijerph-19-06451-g001.jpg
0.410101	bdae97732d0740b587534101fb8b7405	Cumulative emissions reductions in 2025.	PMC9180115	ijerph-19-06451-g002.jpg
0.571548	7ffd2fc1a2d84da7bdc90d9a322b514a	Changes in Fitness Focus by Account Type and Year.	PMC9180174	ijerph-19-06845-g001.jpg
0.405951	664c7fc5e7cc4119b0a4ec9d3a35c6d8	Phenotypic characterization of fatty liver syndrome and liver iron disorder in liver and plasma. (a) Flow chart of the experimental design. (b) HE staining of hepatic sections (n = 3) and ORO staining of hepatic sections (n = 3). (c) Hepatic content of TG (n = 8). (d) Plasma level of TG and NEFA (n = 8). (e) Plasma level of Tch, LDL-C and HDL-C (n = 8). (f) Plasma level of ALT (n = 8). (g) Hepatic content of iron (n = 8). (h) Plasma level of Tf-iron, UIBC and TIBC (n = 8). Values are means ± SEM. # p < 0.05 and ## p < 0.01 compared with the CON group. * p < 0.05 and ** p < 0.01 compared with the HF group.	PMC9180950	ijms-23-06263-g001.jpg
0.415614	ebd379e2a9aa4663a9d263ff840392b3	Hepatic genes expression involved in lipid metabolism. (a) Hepatic mRNA expression of PPARγ (n = 6). (b) Hepatic mRNA expression of CD36 (n = 6). (c) Hepatic mRNA expression of ACC1, FASN and SCD1 (n = 6). (d) Protein content of PPARγ in the liver (n = 6). (e) Protein content of CD36 in the liver (n = 6). (f) Protein content of ACC1, FASN and SCD1 in the liver (n = 6). Values are means ± SEM. # p < 0.05 and ## p < 0.01 compared with the CON group. * p < 0.05 and ** p < 0.01 compared with the HF group.	PMC9180950	ijms-23-06263-g002.jpg
0.407586	3f6ce437b1e445a39e6e302cef7523ce	Hepatic genes expression involved in iron metabolism. (a) Hepatic mRNA expression of iron metabolism (n = 6). (b) Protein content of ZIP14 in the liver (n = 6). (c) Protein content of DMT1 in the liver (n = 6). (d) Protein content of FTL in the liver (n = 6). (e) Protein content of FPN in the liver (n = 6). (f) Hepatic mRNA expression of HAMP and the upstream regulators (n = 6). Values are means ± SEM. # p < 0.05 and ## p < 0.01 compared with the CON group. * p < 0.05 and ** p < 0.01 compared with the HF group.	PMC9180950	ijms-23-06263-g003.jpg
0.421934	b1c8e92d4dfa4d5f9a406429e582b200	Hepatic genes expression involved in methyl-transfer. (a) Hepatic mRNA expression of BHMT (n = 6). (b) Hepatic mRNA expression of GNMT (n = 6). (c) Hepatic mRNA expression of DNMT1 (n = 6). (d) Protein content of BHMT in the liver (n = 6). (e) Protein content of GNMT in the liver (n = 6). (f) Protein content of DNMT1 in the liver (n = 6). Values are means ± SEM. # p < 0.05 and ## p < 0.01 compared with the CON group. * p < 0.05 and ** p < 0.01 compared with the HF group.	PMC9180950	ijms-23-06263-g004.jpg
0.435726	48fbdd47f3fc4951b980db2e69b10655	Hepatic methylation level on promoter of affected genes. (a) Methylation status on the promoter of CD36 gene (n = 3). (b) Methylation status on the promoter of PPARγ gene (n = 3). (c) Methylation status on the promoter of HAMP gene (n = 3). (d) Methylation status on the promoter of BMP2 gene (n = 3). Values are means ± SEM. # p < 0.05 and ## p < 0.01 compared with the CON group. * p < 0.05 and ** p < 0.01 compared with the HF group.	PMC9180950	ijms-23-06263-g005.jpg
0.390848	8f80a0fb196e4e2ca49b5d59afaf62a6	Overview of the hypothesis and validation of anticipatory responses in E. coli MG1655 for carbon sources that have spatial abundances in the mammalian intestine.	PMC9181292	ijms-23-05985-g001.jpg
0.42056	aadeb3f6000e460eb4ae44187ddb2aa0	(a) The spatial abundance of different carbon sources in the intestine; (b) Expected and validated cross-regulation in the intestine. Top left slice, regulation based on the expectation and published knowledge; top right slice, regulation denoted from the RNA-Seq; bottom slice, regulation verified by the RT-PCR. Green, positive regulation; red, negative regulation; grey, no regulation; white, not measured.	PMC9181292	ijms-23-05985-g002.jpg
0.3938	69080fc3288b4cc89f84f875c883113a	Screening of E. coli MG1655 mutants demonstrating a positive association between D-galactose treatment and the expression of malP (a gene of the maltose operon). (a,b) Screening of D-galactose and D-maltose responsive E. coli strains; (c) Further characterization of demonstrating a positive association between D-galactose treatment and the expression of malP, at least in triplicate. The responses reported in plot A were measured in a plate reader, and the rest of the measurements were taken using a flow cytometer. * indicates significant differences (p-value < 0.05), and ns indicates no significant differences (p-value > 0.05). The p values were calculated by the two-sample t-test. Error bars indicate the standard error of the mean (±).	PMC9181292	ijms-23-05985-g003.jpg
0.463336	bf0dc0a6edf4493eb1789b6b9067961b	Identification of mutations and characterization of repair mutants. (a) Mutations in strains demonstrating the upregulation of malP expression in D-galactose supplemented media. EGK5_A8 and EGK8_A7 had point mutations in the coding region of crP, while EGK6_D3 had a point mutation in the promoter region of the malt gene; (b,c) Responses of repair mutants in 0.1% glycerol M9 and 0.1% glycerol M9 supplemented with 10 mM D-galactose. Fold repression was calculated by dividing the gfp (malP) levels in 0.1% glycerol M9 supplemented with 10 mM D-galactose with the gfp (malP) levels in 0.1% glycerol M9. Point mutations in EGK5_A8, EGK8_A7 and EG6_D3 were repaired to generate the repair mutants MING_crP2, MING_crP1 and MING_pmalT1, respectively. * indicates significant differences (p-value < 0.05). The p values were calculated by the two-sample t-test. Error bars indicate the standard error of the mean (±).	PMC9181292	ijms-23-05985-g004.jpg
0.452053	7e0bba1af8a34118a3739e3204d5691f	Competition assays to measure the fitness of lacZ deleted mutant strains showing a positive association between D-galactose treatment and malP expression. (a) Experimental strategy; (b) Fitness of lacZ deleted mutant strains against wild type strain MING.	PMC9181292	ijms-23-05985-g005.jpg
0.407597	c153bf7654cd4bd9b526323ee57a97e4	Fluorescence microscopy studies showing the ability of this technique to delineate the preferential localization of photosensitizing agents.	PMC9181573	ijms-23-06195-g001.jpg
0.487773	f86014e13b0140a69ae8d32e3a7069e0	Photokilling in 3D culture showing the synergistic effect of simultaneously targeting mitochondria and ER with benzoporphyrin derivative (BPD). Free BPD targets mitochondria > ER; ‘anchored’ BPD targets lysosomes. See Ref. [33] for further details.	PMC9181573	ijms-23-06195-g002.jpg
0.451701	4ad37a55a42449c48848e216c545a751	Morphology of apoptosis vs. paraptosis. Phase contrast images are compared with fluorescence-labeling of nuclei with Höchst 33342 [37].	PMC9181573	ijms-23-06195-g003.jpg
0.442121	9e2f915dda844b8aa4bf5233538e6f8c	Particle size distributions of IOT, PS, and SS.	PMC9181601	materials-15-03866-g001.jpg
0.497782	a26174d15e3545daa02372453ba467eb	SEM images of (a) IOTs, (b)PS, and (c)SS.	PMC9181601	materials-15-03866-g002.jpg
0.473069	dab891b8307245a896d417f221104340	The XRD pattern of the IOT.	PMC9181601	materials-15-03866-g003.jpg
0.424826	30da202c26d14a4d9547bd211c7b574c	The influence of (a) SCMs Type, (b) Content of IOT, (c) IOT Grinding Time, and (d) SCMs Content on the compressive strength of concrete.	PMC9181601	materials-15-03866-g004.jpg
0.436247	1f15dc0f7e5546a0a5d2c7d876160da8	Particle size distribution and cumulative sieve allowance of IOTs at different grinding times.	PMC9181601	materials-15-03866-g005.jpg
0.428327	7e3b79a9ed544c0db69616d5c225a0d5	SEM images of (a) Grinding IOTs for 0 h, (b) Grinding IOTs for 1.5 h, (c) Grinding IOTs for 2 h, and (d) Grinding IOTs for 2 h.	PMC9181601	materials-15-03866-g006.jpg
0.479863	f5de3c368ea04214a9224106a4b2bb9a	XPS patterns of IOTs at different grinding times of (a) binding energy of Si2p, (b) binding energy of Al2p, and (c) binding energy of Ca2p.	PMC9181601	materials-15-03866-g007.jpg
0.526773	6ed8738dd78e4f249bf863c90ec5bf90	XRD patterns of IOTs at different grinding times.	PMC9181601	materials-15-03866-g008.jpg

0.40849

301526bf4d1d4d4caab73e4b6392f330

Images for troubleshooting(A) Images of the intermediate lobe (IL) remaining in the anterior lobe side (AL).(B) Images of neuron-like sharp cells in primary culture. The enlarged image is shown in the lower panel.(C) Failure of aggregate formation using low purity adult pituitary stem/progenitor cells. Scale bars: 1 mm (A) and 100 μm (B and C).

PMC9168163

gr8.jpg

0.420738

abdfa40d8af24603921432121ae4551c

A, Clinical image of 5.5-cm × 5.0-cm brown patch with variegated pigmentation involving the right zygomatic area with presence of scar postbiopsy. B, Dermatoscopic image of the scar, showing mild perifollicular hyperpigmentation near the lateral border of the scar.

PMC9168378

gr1.jpg

0.450778

cf2fb96ee39b4d7eb2700428bd0e46dc

A, Reflectance confocal microscopy image (0.75 mm × 0.75 mm, Vivascope 3000; Caliber Imaging and Diagnostics, Inc); location: epidermis. Presence of a predominantly honeycomb pattern and atypical dendritic-shaped pagetoid cells aggregating near follicles (arrows), typical for lentigo maligna. B, The dermoepidermal junction, showing widespread disarray with the presence of dendritic cells (arrows) adjacent to follicles in the background of an unspecified pattern (asterisk).

PMC9168378

gr2.jpg

0.454467

73b847eeeff0445093bb69db675754bb

Area of concern identified with handheld RCM stained with a small drop of surgical dye (arrow) to serve as a landmark for pathologists. Surrounding marks were made using a sterile marker to delineate the exterior position of the confocal probe’s plastic ending, with central area ink stain correlated with the direct central position of cellular atypia on RCM. RCM, Reflectance confocal microscopy.

PMC9168378

gr3.jpg

0.468694

33d97565bddb4806ae821e4ab4877a43

Histologic image showing junctional proliferations of enlarged, atypical melanocytes with hyperchromatic and pleomorphic nuclei arranged in heterogenous nests (stars). Green surgical dye used for landmarking noted along superficial epidermis.

PMC9168378

gr4.jpg

0.399403

f2d2e1d91d6e40df8b403d0a30510a61

Participant selection flow chart. RESCUE-RE refers to a registration study for Critical Care of Acute Ischemic Stroke After Recanalization. mRS: modified Rankin Scale.

PMC9168462

fneur-13-877773-g0001.jpg

0.460222

9f4c5c69a44342e18f86a92f0ab521ce

The mRS score at 3 months. mRS, modified Rankin Scale.

PMC9168462

fneur-13-877773-g0002.jpg

0.445535

ae382e2790e44fc3820b7e3b3aed596a

Subgroup analysis of the primary outcome. The forest plot shows the differences in odds ratio for primary outcome (modified Rankin Scale [mRS], 0–2) at 90 days in subgroups. Adjusted for age, sex, diabetes mellitus, hypertension, atrial fibrillation, dyslipidemia, smoking, education, insurance, time from stroke onset to admission interval, mRS before the stroke, baseline NIHSS score, NIHSS score 24h after EVT, the side of the brain where the stroke occurred, stroke cause, TICI score of 2b or 3. OR, odds ratio; CI, confidence interval; mRS, modified Rankin Scale; TICI, thrombolysis in cerebral infarction; NIHSS, National Institute of Health Stroke Scale.

PMC9168462

fneur-13-877773-g0003.jpg

0.409124

7eba018fd4aa4f7eb2bd48e9da7650fa

Patient selection, exclusion, and inclusion criteria. PMF, posterior malleolus fracture.

PMC9168892

10.1177_10711007211070540-fig1.jpg

0.430648

2c229590c0a84928abd9173a1d752c24

Histograms of the distribution of Self-Reported Foot and Ankle Score (SEFAS) in patients treated within (upper panel) and after (lower panel) a week from injury.

PMC9168892

10.1177_10711007211070540-fig2.jpg

0.505475

42af7c2f09664767814e96ae110b0e54

Diagnosis and treatment strategies for the neonatal outpatient service and emergency room during the coronavirus disease 2019 pandemic. COVID-19: Coronavirus disease 2019. Ma LS, Zhao YL, Wei YD, Liu C. Recommendations for perinatal and neonatal surgical management during the COVID-19 pandemic. World Journal of Clinical Cases. 2020;8(14):2893–901. This figure is from a previous publication by our team

PMC9170880

383_2022_5136_Fig1_HTML.jpg

0.543159

3a818c75937a49128ba53e693f60ca3e

Transportation strategies for severe and critically ill patients from the neonatal surgery department during the coronavirus disease 2019 pandemic. COVID-19: Coronavirus disease 2019; SARS-CoV-2: Severe acute respiratory syndrome coronavirus 2. Ma LS, Zhao YL, Wei YD, Liu C. Recommendations for perinatal and neonatal surgical management during the COVID-19 pandemic. World Journal of Clinical Cases. 2020;8(14):2893–901. This figure is from a previous publication by our team

PMC9170880

383_2022_5136_Fig2_HTML.jpg

0.433649

c009c900ba654c91aca43a1af17aa0ac

Neonatal emergency surgery procedures in the neonatal surgery department during the coronavirus disease 2019 pandemic. COVID-19: Coronavirus disease 2019; CT: Computed tomography. Ma LS, Zhao YL, Wei YD, Liu C. Recommendations for perinatal and neonatal surgical management during the COVID-19 pandemic. World Journal of Clinical Cases. 2020;8(14):2893–901. This figure is from a previous article by our team

PMC9170880

383_2022_5136_Fig3_HTML.jpg

0.38921

35e451f579d64db0b19f05691130cc92

Study flow diagram. CDH congenital diaphragmatic hernia; NTG Normal time group; STG Special time group

PMC9170880

383_2022_5136_Fig4_HTML.jpg

0.42008

9f153f29bc7a451593875ef278073f12

Overall photo of case 1, a 2.5-year-old girl.

PMC9171574

aqab204f0001.jpg

0.419952

87cf6eb398b54305bf455d600f39fe5f

Overall photo of case 2, a 4-year-old girl.

PMC9171574

aqab204f0002.jpg

0.475149

1ba50dd5da954886b6db38b4226f44f9

Overall photo of case 3, a 10-year-old girl.

PMC9171574

aqab204f0003.jpg

0.51496

0ca4a501152e4cf0889681d8f5c3c48e

Scalp of case 1.

PMC9171574

aqab204f0004.jpg

0.480457

a0ceb581a84f4ac8af088052b37e2ded

Thymic involution of each case (A, C, E), with thymus of age-matched controls (B, D, F). (Glass microscope slides stained with H&E, all photographed with ×2.5 camera macro lens.)

PMC9171574

aqab204f0005.jpg

0.537102

d528576bca8b4aafaec2cbd2c897897d

Low-power views depicting myocardium with diffuse hypereosinophilia in 3 cases (H&E, ×10 objectives). A, Case 1; B, case 2; C, case 3.

PMC9171574

aqab204f0006.jpg

0.430963

cd0f9ae29bb84d43b29629b5983f2abc

Higher-power photomicrographs depicting myocyte catecholamine necrosis in 3 cases, including contraction banding, hypereosinophilia, myofibrillar disarray and dissolution, cytoplasmic vacuolization, and interstitial edema. A, Case 1; B, C, D, case 2; E, F, case 3. Cases 2 and 3 also demonstrate focal myocyte hypertrophy. (H&E; A, B, ×40 objectives; C, D, E, F, ×60 objectives).

PMC9171574

aqab204f0007.jpg

0.456

ce528dd50d1a4317b8231574bc0eaa81

Scalp of case 2.

PMC9171574

aqab204f0008.jpg

0.451572

1ed4eeba29e14be5a2270fb6a6865b41

Anitschkow-like chromatin patterns in myocardium from case 2 (A, B, C) and case 3 (D, E, F). A-E, Longitudinal sections display “caterpillar” pattern of nuclear chromatin, appearing to be of the myocytes. Slightly tangential sections of nuclei are in interstitial cells (arrows). F, Two transverse sections of nuclei display the “owl eye” pattern (arrows). (H&E, original objective magnifications ×60)

PMC9171574

aqab204f0009.jpg

0.440418

1bd24284cc84453c971d0a6c67576c46

Scalp of case 3.

PMC9171574

aqab204f0010.jpg

0.421

3eb2fc198a644d42a2423abed6039c4b

Overall photo of consult case 3.75-year-old girl.

PMC9171574

aqab204f0011.jpg

0.480971

df40dbb72b5049a69b791d2a74ba76f6

Scalp of consult case.

PMC9171574

aqab204f0012.jpg

0.478118

6c909ca82511460da702b35d3a9bba1c

Thymus of older patients depicting normal age-related involution. A, Well-preserved distinction between cortex and medulla despite overall decrease of parenchyma. B, Interstitium with fatty infiltration of septa. (Glass microscope slides stained with H&E, both with ×3.0 camera macro lens)

PMC9171574

aqab204f0013.jpg

0.43555

cf4cfe7dc0534d9c89f082464ffc0ef4

Animals and information included in the genetic prediction scenarios. a Shows the relationship between purebred and crossbred pigs, as well as the number of phenotypes/genotypes collected for purebred and crossbred parents and offspring. b Shows the source of phenotypic information used for the genomic prediction analysis for each phenotyping scenario, with the diagram showing an example where crossbred offspring of group-4 are the animals to be predicted. c Shows the sources of genomic information for each genotyping scenario. The figure was created with BioRender.com

PMC9171933

12711_2022_736_Fig1_HTML.jpg

0.420689

cbead453d4394daeaed77136a32bf5b7

Estimates of genetic correlations between crossbred and purebred traits. The plot shows the strength and direction of the genetic correlations between purebred and crossbred traits. Darker colors indicate a stronger correlation, while red and purple indicate a positive and negative correlation, respectively

PMC9171933

12711_2022_736_Fig2_HTML.jpg

0.427156

1774c384647945558ca47bdfcc56cf23

Estimates of genetic correlations between the crossbred traits in the breeding objective in selection index for scenarios CB-Live, CB-FOM, and CB-Color. The plot shows the strength and direction of the genetic correlations between the breeding objectives and crossbred traits in the selection criteria. Darker colors indicate a stronger

PMC9171933

12711_2022_736_Fig3_HTML.jpg

0.424498

9dc899454f81406a818a870d786af47a

First two principal components for variation in the genomic relationship matrix

PMC9171933

12711_2022_736_Fig4_HTML.jpg

0.45605

6089cc811c2841f3808c1df8e2606142

Accuracy of (genomic) prediction using various genotyping strategies in the crossbred phenotyping scenarios. Each panel represents a breeding objective. The x-axis shows the phenotyping scenario (ST/CB-Live/CB-FOM/CB-Color/PB-1/PB-2/PB-3), and the y-axis shows the accuracy obtained. Genotyping scenarios are represented by shape/color combinations: black circle (no genotyping), brown triangle (Stage-1), red square (Stage-2), and purple line (Stage-3). For the definitions of the phenotyping scenarios, please refer to the “Indirect selection for carcass traits using purebred growth phenotypes” and “Less expensive crossbred phenotypes for the selection of meat quality” sections. For the definitions of the genotyping scenarios, please refer to the “Prediction of crossbred traits” section

PMC9171933

12711_2022_736_Fig5_HTML.jpg

0.456933

2b2260bba3a9450884359c7379658b61

Accuracy of (genomic) prediction using various genotyping strategies in the purebred phenotyping scenarios. Each panel represents a breeding objective. The x-axis shows the phenotyping scenario (ST/PB-1/PB-2/PB-3), and the y-axis shows the accuracy obtained. Genotyping strategies are represented by shape/color combinations: black circle (no genotyping), brown triangle (Stage-1), red square (Stage-2), and purple line (Stage-3). For the definitions of the phenotyping scenarios, please refer to the “Indirect selection for carcass traits using purebred growth phenotypes” and “Less expensive crossbred phenotypes for the selection of meat quality” sections. For the definitions of the genotyping scenarios, please refer to the “Prediction of crossbred traits” section

PMC9171933

12711_2022_736_Fig6_HTML.jpg

0.438863

2d4fb076814146f7a4f17a42448cfffa

CFS + LM suspension to correct unilateral congenital blepharoptosis. a: Preoperative image of a five-year-old boy with congenital blepharoptosis OD; b: incision line labeled with methylene blue; c: exposure of CFS and LM; d: image of the patient twenty-two months after CFS + LM suspension

PMC9175472

12886_2022_2469_Fig1_HTML.jpg

0.418778

6b0c2d20fb6541db9bc8fec1ec1fa719

Microscopic images of the CFS sections. (Victoria Blue staining, × 10)

PMC9175472

12886_2022_2469_Fig2_HTML.jpg

0.378095

7807f4534cfd4d4ea35af043d54c5084

Results of immunofluorescent staining of the CFS and LM in the three age groups. (NC: negative contrast)

PMC9175472

12886_2022_2469_Fig3_HTML.jpg

0.451826

d455febb1dc64461828b23d5528c9742

Western blot showing elastin/β-actin expression in the CFS and LM in the three age groups

PMC9175472

12886_2022_2469_Fig4_HTML.jpg

0.561385

0aceac952118453bbfe55d0aaf325395

Elastin expression in the CFS and LM of all groups. A: child group; B: adolescent group; C: adult group; D: elastin expression of CFS in the child, adolescent and adult groups. (* P < 0.05, ** P < 0.01)

PMC9175472

12886_2022_2469_Fig5_HTML.jpg

0.458724

efd41f8b96a54bceaad3a5ad2788442c

Western blot of elastin expression in the CFS in the child, adolescent and adult groups

PMC9175472

12886_2022_2469_Fig6_HTML.jpg

0.42478

db53749c9f2e4ce0b3cf634610ae0d11

Structure of RBM.

PMC9177386

fpsyg-13-843427-g001.jpg

0.480934

e6efc82230974d34bc483cb995de92cc

Comparison between time-domain waveform after pre-emphasis of pipa music segment and the original time-domain waveform.

PMC9177386

fpsyg-13-843427-g002.jpg

0.454426

a183c073cc8a41bfa3ab27aa3bd3bd2b

Framing diagram of the pipa music clip.

PMC9177386

fpsyg-13-843427-g003.jpg

0.482973

c30145bd21aa4da584f117811e34857f

The standard MFCC parameter extraction process for traditional instrumental music.

PMC9177386

fpsyg-13-843427-g004.jpg

0.507499

b14b9742393c4e0cabd8aaaa14225fa4

The first-dimensional MFCC characteristic parameters of the guzheng music segment.

PMC9177386

fpsyg-13-843427-g005.jpg

0.480051

6899851e276443908cf7178277347a6e

The DBN-based structure of the national musical instrument recognition and differentiation network.

PMC9177386

fpsyg-13-843427-g006.jpg

0.432956

b8986d3cefe54b5d803efe1369fcbe2c

The retrieval framework of differentiated music library platform.

PMC9177386

fpsyg-13-843427-g007.jpg

0.469515

bafbfb603ca8408db4733a8b4f4bbdef

Comparison of prediction accuracy of different hidden layers for national musical instruments.

PMC9177386

fpsyg-13-843427-g008.jpg

0.456178

d85ae7fcb7784533b3fdb959c3b1d5f9

The average accuracy of the discrimination algorithm based on the DBN and traditional discriminator for national musical instrument recognition.

PMC9177386

fpsyg-13-843427-g009.jpg

0.453617

03395e05d36141388c06cf9ecaead624

The confusion matrix composed of recognition rates [(A) recognition rate confusion matrix of distinguishing national musical instruments by distinguishing trees; (B) recognition rate confusion matrix of distinguishing national musical instruments by KNN].

PMC9177386

fpsyg-13-843427-g010.jpg

0.465217

987f7850345b4329854fc4e17c0cc207

The confusion matrix of recognition accuracy [(A) the recognition rate confusion matrix of SVM for distinguishing the national musical instruments; (B) the recognition rate confusion matrix of softmax for distinguishing the national musical instruments].

PMC9177386

fpsyg-13-843427-g011.jpg

0.414318

dac59b52cf8d4fe8a2f8307c3f682e1e

The confusion matrix of recognition accuracy of the DBN and softmax in distinguishing the national musical instruments.

PMC9177386

fpsyg-13-843427-g012.jpg

0.419265

976f737aac21498ab40f34eecf1794aa

(A) Phenotypes of the Williams 82, Gmpgl3-1, and Gmpgl3-2 mutants. Scale bars, 5 cm. (B–G) Pigment contents and photosynthesis parameters of the Williams 82, Gmpgl3-1, and Gmpgl3-2 mutants. (B) Chlorophyll a (Chl a), chlorophyll b (Chl b) and carotenoid (Car). (C) Chlorophyll fluorescence parameter (Fv/FM). (D) Photosynthetic rate (Pn). (E) Transpiration rate (Tr). (F) Stomatal conductance (Gs). (G) Intercellular CO2 concentration (Ci). (H) Chloroplast structure in Williams 82. (I,J) Chloroplast structure in the Gmpgl3-1 and Gmpgl3-2 mutants. Scale bars, 1 μm. *** represents significant differences compared with the control (Williams 82) at p < 0.001, and the error bars represent standard deviations.

PMC9178232

fpls-13-892077-g001.jpg

0.484644

080afd73bdec44b7a7d25a8bcc7d494a

Map-based cloning of the GmTic110a locus. (A) SNP index plot of all chromosomes of the F2 plants. (B) SNP index plots of chromosome 02 of the Gmpgl3a mutant from the F2 population. (C) Physical position of the GmTic110a candidate gene. (D) Schematic diagram showing the structure of GmTic110a. The red lines indicate mutation sites within the GmTic110a gene in the two mutant lines.

PMC9178232

fpls-13-892077-g002.jpg

0.416064

2a1fb6170a5f4a9ebf9c094731384284

(A) Multiple sequence alignments of the Tic110 protein in Arabidopsis thaliana (At), Glycine max (Gm), Physcomitrella patens (Pp), Chlamydomonas reinhardtii (Cr), and Pisum sativum (Ps). The dark region represents identical amino acids, and the grey region represents similar amino acids. (B) Schematic diagram representations of the two structural models of the Tic110 protein. In terms of the locations of the proposed TM domains, the red boxes represent TM1 and TM2, the orange boxes represent TM3 to TM6, the blue boxes represent transit peptides (TPs), the green box represents TP binding, and the yellow box represents a co-domain. (C) Phylogenetic trees based on the multiple sequence alignments of the Tic110 proteins. Bootstrap values from 1,000 replicates are indicated at each node. (D) Conserved motifs of Tic110 proteins in G. max, A. thaliana, Medicago sativa, Oryza sativa, Zea mays, Sorghum bicolor, Selaginella tamariscina, and P. patens were identified using the MEME search tool. Different motifs (1–12) are represented by boxes with different colors. (E) Tissue-specific expression profiles were determined via qRT–PCR.

PMC9178232

fpls-13-892077-g003.jpg

0.479946

42c40989bad1462b80e9fd16ca16b902

(A) Phenotypes of Williams 82, the Gmpgl3 mutant, and CRISPR/Cas9-edited plants (GmTic110aCR1, GmTic110aCR,2 and GmTic110aCR3). Scale bar = 1 cm. (B) sgRNA target sequences of Williams 82, GmTic110aCR1, GmTic110aCR2, and GmTic110aCR3. The sgRNA target sequence is shown in blue letters, and the protospacer-adjacent motif (PAM) site is shown in yellow letters. The red letters indicate a single-base substitution. – indicates a deletion of the corresponding nucleotide. (C) Relative expression of the GmTic110a gene in unifoliate leaves of Williams 82, the Gmpgl3 mutant, and CRISPR/Cas9-edited plants. The asterisks indicate statistically significant differences, as determined by Student’s t-test (***, p < 0.001), and the error bars represent the standard deviations.

PMC9178232

fpls-13-892077-g004.jpg

0.445248

8166af78f1b94120b97e2ecc9f8c9296

Subcellular localization of GmTic110a, GmTic110aG114A, Gmtic110aT805S, GmTic110aCR1, GmTic110aCR2, and GmTic110aCR3. (A-G) Transient expression of GFP-GmTic110a, GFP-GmTic110aG114A, GFP-Gmtic110aT805S, GFP-GmTic110aCR1, GFP-GmTic110aCR2, GFP-GmTic110aCR3 and GFP in Arabidopsis protoplasts. GFP, GFP fluorescence; Chlorophyll, chlorophyll autofluorescence; Bright, bright field. Merged, merged image of GFP fluorescence, chlorophyll autofluorescence and bright field images. Scale bars = 10 μm.

PMC9178232

fpls-13-892077-g005.jpg

0.430199

2a56002866d848368cebded623855ef6

GmTic110a interacts with GmTic20, GmTic40a, and GmTic40b. (A,C,E) Luciferase complementation assay showing that GmTic110a interacts with GmTic20, GmTic40a, and GmTic40b in Nicotiana benthamiana. Luciferase activity was detected 3 days after injection. (B,D,F) Interactions with GmTic20, GmTic40a, and GmTic40b in N. benthamiana according to a Co-IP assay. Immunoblots of the total protein extracts (20% input) and the immunoprecipitation product were performed using an anti-HA antibody (a-HA) or an anti-FLAG antibody (a-FLAG), respectively.

PMC9178232

fpls-13-892077-g006.jpg

0.39569

540f3664852245ef9049c83d4cbb9809

Distribution diagrams of tea plants used in this study and results of paternity assignments. a EW plantation for seed collection and the tea cultivars surrounding it. b EW offspring planting in the Mingshan experimental field. c ASR distribution for 14 candidate fathers. d Distribution of ssASR of each offspring with the 14 hypothetical parental couples. e Distribution of trio LOD scores of the 392 offspring when set with EW × CM as a parental couple. f Venn diagram showing the common results provided by the three paternity assignment methods for the EW × CM couple.

PMC9178331

uhac086f1.jpg

0.434741

4a6bf56c55354750aa6712aabbee44ce

Results of population structure analyses of the samples tested in this study. a Population structure obtained from admixture when K = 5 and 10. b Scatter plot based on the first two principal components (PCs) of the principal component analysis.

PMC9178331

uhac086f2.jpg

0.419939

1b4f39408e0a4d91bc83a402c3aed1bf

High-density linkage map constructed using genotypic data of the 217 full-sib offspring of EW × CM. a Distribution of SNP markers on the 15 linkage groups. b Collinearity analysis of the genetic positions of markers on the map and physical positions in the reference genome. c Heat maps showing distances and recombination rates between markers. The rates of recombination increase from yellow to purple. Gray indicates no available value for the recombination rate between the two markers.

PMC9178331

uhac086f3.jpg

0.38882

464bdd91d0ec4744bcc3e2ab7e8179e4

Phenotypic variation of SPI among the offspring in two consecutive years. a Representative pictures of tea buds with different SPIs. The SPI value is given in each picture. b and c Distribution of the 388 EW offspring based on SPI_2020 and SPI_2021. d and e Comparisons of average SPI values in different crosses. The significances of difference between two groups are indicated by P values from Kruskal–Wallis tests.

PMC9178331

uhac086f4.jpg

0.413981

213142ea713542cf9cef699c4cf47ee6

QTL mapping results with the SPI trait in the EW × CM full-sib population. a LOD value for 2020 and 2021 covering the whole genome by interval mapping. b–d MQM mapping results in Chr3 and Chr4. Genome-wide LOD thresholds at 99 and 95% for QTL claiming are shown by dotted lines. Cofactors used for MQM mapping are indicated by arrows in b–d.

PMC9178331

uhac086f5.jpg

0.389057

9d87c125dd404b379d63c8287717fce7

Characteristics of wecB::Cm mutant of S. Gallinarum. (A) Growth curves of wild-type and wecB::Cm mutant in Luria-Bertani (LB) broth. The bacteria grown in LB broth for 14 h were diluted to OD600 = 1.5 and inoculated 1/100 into the LB broth and cultured at 37°C with shaking (150 rpm). The optical density (OD600) was measured at 0, 2, 4, 6, 8, 10, 12, and 24 h after inoculation. (B) Bacterial counts of wild-type and wecB::Cm mutant in LB broth. The bacteria were cultivated, as described above. At the indicated time points, 100 μl of each culture was serially diluted in LB broth and 100 μl of each dilution was spread on an LB agar plate. After incubating overnight at 37°C, count the colonies on the plate as colony forming units (CFU). (C–E) Bacterial growth characteristics of wecB::Cm mutant in LB broth containing bile acid (C), hydrogen peroxide (D), or nalidixic acid (E). The bacteria were grown in LB broth for 14 h and then diluted to 2.0 ×107 CFU/ml. The diluted bacterial culture (50 μl) was mixed with 50 μl of each reagen in 96-well flat-bottom plates and incubated at 37°C without shaking. The optical density (OD595) was measured at 0, 2, 4, 6, 8, 10, and 24 h after inoculation. The data are means ± standard deviations based on six wells per group at each time point. The significant differece was shown as **p < 0.01.

PMC9178343

fmicb-13-880932-g0001.jpg

0.431465

51d1707d433748efa349209330131a56

Pathological finding in the chickens orally infected with wild-type or wecB::Cm mutant strains. (A) Survival curve of chickens orally inoculated with wild-type or wecB::Cm mutant. Chickens were orally inoculated with 108 CFU of wild-type or wecB::Cm mutant, and survival of the chickens was recorded for 14 days post-infection. (B) Gross lesions in the liver of chickens orally inoculated with wild-type or wecB::Cm mutant. The livers of pathological changes were observed on 3, 5, and 7 days post-infection (dpi). White lesions and small necrotic foci were observed in wild-type infected chickens on 3 days after infection, and liver hypertrophy and congestion were observed on 7 days after infection. In the chickens infected with wecB::Cm mutant, although slight white lesions and small necrotic foci were observed on 5 days after infection, no hypertrophy or swelling was observed.

PMC9178343

fmicb-13-880932-g0002.jpg

0.598283

62084556484145ea97ce25e3faee3729

Viable bacterial counts in the liver and spleen of chickens orally inoculated with wild-type or wecB::Cm mutant. Chickens were orally inoculated with 108 CFU of wild-type or wecB::Cm mutant. The bacteria in the liver and spleen were determined on 1, 3, 5, and 7 days post-infection. The data are means ± standard deviations based on three to six chickens per group at each time point. The significant differences were shown as **p < 0.01.

PMC9178343

fmicb-13-880932-g0003.jpg

0.412989

9405357809494af297f0911228031689

Histopathological changes and microscopic lesions in the livers of chickens orally infected with wild-type or wecB::Cm mutant strain. Chickens were orally inoculated with 108 CFU of wild-type or wecB::Cm mutant and the livers were collected on 3, 5, and 7 days post-infection. The organs of uninfected chickens were used as the controls. Paraffin sections of the organs were prepared and stained with hematoxylin-eosin (HE). (A) Magnification 100 × and (B) magnification 400 × . Arrows show lesions which were characterized by marked infiltration of heterophils and lymphocytes with degeneration and necrosis.

PMC9178343

fmicb-13-880932-g0004.jpg

0.490589

3c304d09c77c4aeb959dbd8991aa7b46

Histopathological changes and microscopic lesions in the spleens of chickens orally infected with wild-type or wecB::Cm mutant strain. Chickens were orally inoculated with 108 CFU of wild-type or wecB::Cm mutant and the livers were collected on 3, 5, and 7 days post-infection. The organs of uninfected chickens were used as the controls. Paraffin sections of the organs were prepared and stained with HE. (A) Magnification 100 × and (B) magnification 400 × . Arrows show degeneration and necrosis in white pulp.

PMC9178343

fmicb-13-880932-g0005.jpg

0.457589

1ac5cc2cc1554e36b62e1103e999a6e1

Expression of cytokine and chemokine in the liver (A) and spleen (B) of chickens infected with wild-type or wecB::Cm mutant strains. Chickens were inoculated orally with 108 CFU of wild-type or wecB::Cm mutant. The liver and spleen of the chickens were collected on 3 and 5 days post-infection, and the expression of interleukin-1β, IL-6, tumor necrosis factor-α, interferon-γ, IL-12, and CXCLi1 was determined by quantitative RT-PCR. Data were expressed as means ± standard deviations of fold changes in gene expression of the organs from infected groups relative to those from the uninfected control group (three chickens per group at each time point). Statistical analysis was performed using one-way ANOVA analysis followed by Tukey's multiple comparison test to compare infected chickens with uninfected controls. The significant differences were shown as *p < 0.05, **p < 0.01.

PMC9178343

fmicb-13-880932-g0006.jpg

0.414154

9a24c29def9a48d69efb837543f1b695

Kinetics of bacterial colonization and protective immune responses in the chickens orally infected with wecB::Cm mutant. (A) Viable bacterial numbers in the liver and spleen of chickens infected with wild-type or wecB::Cm mutant strains. Chickens were orally inoculated with 108 CFU of wild-type or wecB::Cm mutant, and the number of bacteria in the organs was determined on 1, 3, 5, 7, 11, 20, 25, 35, and 45 days post-infection. The data are means ± standard deviations based on three to six chickens per group at each time point. (B) Antibody production in chickens infected with wecB::Cm mutant strain. Five Chickens were orally inoculated with 108 CFU of wecB::Cm mutant, and the anti-S. gallinarum antibodies were determined by serum agglutination test on 45 days after infection. PBS and serum from uninfected chickens (n = 5) were used as a negative control, and anti-O9 serum was used as a positive control. (C) Survival curve of chickens orally inoculated with wecB::Cm mutant and re-challenged with wild-type strain on 35 days after initial inoculation. wecB::Cm-inoculated chickens were re-challenged with 108 CFU of the wild-type strain, and the survival of chickens was recorded for 14 days after re-infection with the wild-type strain.

PMC9178343

fmicb-13-880932-g0007.jpg

0.422678

3a1b85894690493f81c266f0a9fd6f42

Anti-spike and anti-RBD titers after primary vaccination and boosting in individuals with PAD(A and B) Anti-spike (A) and RBD (B) (ancestral strain) endpoint titers in 48 lots of 6 different immunoglobulin replacement products (squares) compared with 20 HD (blue circles) before and 14 and 90 days after completion of the BNT162b2 vaccine series.(C and D) Anti-spike (C) and RBD (D) endpoint titers in HDs (n = 20; blue circles), COVID-19-naive individuals with PAD (n = 18, red circles), and COVID-19-experienced individuals with PAD (n = 9, green circles) before or 14 and 90 days after completion of a mRNA vaccination series (BNT162b2, n = 19; mRNA-1273, n = 8).(E and F) Anti-spike (E) and RBD (F) endpoint titers in COVID-19-naive individuals with PAD (n = 16, red circles) and COVID-19-experienced individuals with PAD (n = 3, green circles) before (n = 6) and 14 or 28 (n = 19), 90 (n = 18), and 150 (n = 12) days after completion of a primary mRNA (BNT162b2, n = 14; mRNA-1273, n = 3) or Ad26.COV2.S (n = 2) vaccine series and 14 (n = 19) days and 90 (n = 13) days after booster with a mRNA vaccine (BNT162b2, n = 16; mRNA-1273, n = 3). A dotted black line represents the limit of detection (1/50).(G and H) Anti-spike avidity index in HDs (n = 19, blue circles), COVID-19-naive individuals with PAD (red circles), and COVID-19-experienced individuals with PAD (green circles) 14 and 90 days after primary vaccination (n = 16, COVID-19-naive; n = 6, COVID-19-experienced), 150 days after primary vaccination (n = 10, COVID-19-naive; n = 2, COVID-19-experienced), 14 days after boosting (n = 12, COVID-19-naive; n = 3, COVID-19-experienced), and 90 days after boosting (n = 10, COVID-19-naive; n = 1, COVID-19-experienced).Numbers above graphed data (C–F) represent the geometric mean titer (GMT) for each time point and average avidity index (G and H). Bars indicate median (A and B) values. Kruskal-Wallis with Dunn’s post-test (A–F), paired t test (G), and one-way ANOVA with Dunnett’s post-test (H). Only significant differences are shown: ∗p < 0.05, ∗∗p < 0.01, ∗∗∗p < 0.001, ∗∗∗∗p < 0.0001. See also Figure S1 and Tables S1–S3.

PMC9179023

gr1.jpg

0.389805

d90b826706314d14bcbcfcefe27a73d0

IgG subclasses and FcγR-binding activity of anti-spike antibodies in serum from vaccinated individuals with PAD(A–J) Levels of IgG1(A), IgG2 (B), IgG3 (C), IgG4 (D), IgA (E), IgM (F), FcγR2A-binding (G), FcγR2B-binding (H), FcγR3A-binding (I), and FcγR3B-binding (J) ancestral (S, S1, S2, and RBD), B.1.351, and B.1.617.2 spike antibodies in HDs (n = 20, blue circles), COVID-19-naive individuals with PAD (n = 18, red circles), and COVID-19-experienced individuals with PAD (n = 9, green circles) 14 days after completion of the second dose of the mRNA vaccine series. Two-way ANOVA with Tukey post-test, mean. Only significant differences are shown: ∗p < 0.05, ∗∗p < 0.01, ∗∗∗p < 0.001, ∗∗∗∗p < 0.0001). See also Figure S2 and Table S3.

PMC9179023

gr2.jpg

0.480591

c967b4381004465b84307a86f0b1b520

Neutralizing antibody responses in individuals with PAD after vaccination and boosting(A and B) Serum neutralizing activity against WA1/2020 (A) or B.1.617.2 (Delta) (B) in COVID-19-naive (red circles) and COVID-19-experienced (green circles) individuals with PAD before (n = 4, COVID-19-naive; n = 5, COVID-19-experienced) and 14 (n = 18, COVID-19-naive; n = 9, COVID-19-experienced) or 90 (n = 17, COVID-19-naive; n = 6, COVID-19-experienced) days after mRNA vaccination and 14 days after mRNA booster (n = 14, COVID-19-naive; n = 3, COVID-19-experienced). Shown is the neutralizing activity of immunoglobulin replacement products (n = 17, purple squares).(C and D) Effect of boosting on serum neutralization of WA1/2020 (C) and B.1.617.2 (D) in COVID-naive (n = 16, red circles) and COVID-19-experienced (n = 3, green circles) individuals with PAD after completion of the primary mRNA (14 or 90 days after vaccination; BNT162b2, n = 14; mRNA-1273, n = 3) or Ad26.COV2.S (28 or 90 days after vaccination, n = 2) vaccine series and 14 (n = 19) or 90 (n = 13) days after boosting with a mRNA vaccine (BNT162b2, n = 16; mRNA-1273, n = 3).(E–G) Effect of variant strains on serum neutralizing activity of individuals with PAD 14 days after completion of mRNA vaccination (E) (n = 27 total; n = 18, COVID-19-naive, red circles; n = 9, COVID-19-experienced, green circles), 14 days after boosting (F) (n = 19 total; n = 16, COVID-19-naive, red circles; n = 3, COVID-19-experienced, green circles), and 90 days after boosting (G) (n = 13 total; n = 12 COVID-19-naive, red circles; n = 1, COVID-19-experienced, green circle).LOD, limit of detection. A dotted black line represents the presumptive protective titer as described.28 Numbers immediately above the x axis indicate the number and percentage of individuals with serum-neutralizing titers above 50 at each time point. Numbers above graphed data represent the GMT for each time point. Kruskal-Wallis with Dunn’s post-test. Only significant differences are shown: ∗p < 0.05; ∗∗p < 0.01; ∗∗∗p < 0.001; ∗∗∗∗p < 0.0001). See also Figure S3 and Table S3.

PMC9179023

gr3.jpg

0.409194

106e2def98984f10ae421861c30ecf47

Primed and naïve hESC analysis and XIST expression. (A) Schematic representation of the pipeline of the analysis of expression of X-linked genes and their allelic expression. (B) XIST expression by TPM (transcripts per million) values in primed (blue) and naïve (red) hESCs across 9 different studies. The whiskers were calculated by the default ggplot code in R (upper whisker: min(max(x)), Q3 + 1.5 × IQR, lower whisker: max(min(x)), Q1 − 1.5 × IQR, where Q3 and Q1 are the third and first quartiles, and IQR is Q3 − Q1). The dots represent the outliers. (C) Distribution of XIST status changes across all naïve samples.

PMC9179337

cells-11-01729-g001.jpg

0.393926

2c76225e2cfb44f389722165697df30c

Changes in gene and allelic expression in chromosome X. (A) Average TPM expression of genes in chromosome X of primed (blue) and naïve (red) hESCs across 9 different studies. Whiskers and dots were calculated as mentioned in the legend to Figure 1. (B) Distribution of X-linked gene expression variations between naïve samples and their primed counterparts. (C) Average TPM expression of genes on chromosome X of primed (blue) and naïve (red) hESCs. p-value was calculated by a paired t-test. (D) Average X:autosomes allelic ratio of primed and naïve cells. p-value was calculated by a paired t-test. (E) Distribution of allelic expression variation across all naïve samples.

PMC9179337

cells-11-01729-g002.jpg

0.474278

cef59072112043b2a3fc51eaffe8372b

Geographical distribution of gene and allelic expression along chromosome X. (A) Moving average plot of median log2 gene expression naïve/primed ratio along chromosome X, separated into XIST+ (brown) and XIST− (green) samples. The blue line marks the location of the centromere, and the red line marks the location of XIST. The dashed lines define Xq22. (B) Chromosome X gene expression differences between XIST+ and XIST− samples in the entire X chromosome and in Xq22 area. p-value was calculated by a paired t-test. (C) X:autosomes allelic ratio in the entire chromosome X (light blue) versus Xq22 region (pink) in XIST+ samples. p-value was calculated by a paired t-test.

PMC9179337

cells-11-01729-g003.jpg

0.43849

d84f541138d348cdb45711e2e665c368

PRISMA flow diagram of the study selection process. From: Page, M.J.; McKenzie, J.E.; Bossuyt, P.M.; Boutron, I.; Hoffmann, T.C.; Mulrow, C.D.; Shamseer, L.; Tetzlaff, J.M.; Akl, E.A.; Brennan, S.E.; et al. The PRISMA 2020 statement: an updated guideline for reporting systematic reviews. BMJ 2021, 372, n71. doi: 10.1136/bmj.n71 [17]. For more information, visit: http://www.prisma-statement.org/ (accessed on 1 April 2022).

PMC9179989

ijerph-19-06378-g001.jpg

0.436217

54efa25940de48589d7161aa9bfdbeae

Jingning county ferry distribution map.

PMC9180115

ijerph-19-06451-g001.jpg

0.410101

bdae97732d0740b587534101fb8b7405

Cumulative emissions reductions in 2025.

PMC9180115

ijerph-19-06451-g002.jpg

0.571548

7ffd2fc1a2d84da7bdc90d9a322b514a

Changes in Fitness Focus by Account Type and Year.

PMC9180174

ijerph-19-06845-g001.jpg

0.405951

664c7fc5e7cc4119b0a4ec9d3a35c6d8

Phenotypic characterization of fatty liver syndrome and liver iron disorder in liver and plasma. (a) Flow chart of the experimental design. (b) HE staining of hepatic sections (n = 3) and ORO staining of hepatic sections (n = 3). (c) Hepatic content of TG (n = 8). (d) Plasma level of TG and NEFA (n = 8). (e) Plasma level of Tch, LDL-C and HDL-C (n = 8). (f) Plasma level of ALT (n = 8). (g) Hepatic content of iron (n = 8). (h) Plasma level of Tf-iron, UIBC and TIBC (n = 8). Values are means ± SEM. # p < 0.05 and ## p < 0.01 compared with the CON group. * p < 0.05 and ** p < 0.01 compared with the HF group.

PMC9180950

ijms-23-06263-g001.jpg

0.415614

ebd379e2a9aa4663a9d263ff840392b3

Hepatic genes expression involved in lipid metabolism. (a) Hepatic mRNA expression of PPARγ (n = 6). (b) Hepatic mRNA expression of CD36 (n = 6). (c) Hepatic mRNA expression of ACC1, FASN and SCD1 (n = 6). (d) Protein content of PPARγ in the liver (n = 6). (e) Protein content of CD36 in the liver (n = 6). (f) Protein content of ACC1, FASN and SCD1 in the liver (n = 6). Values are means ± SEM. # p < 0.05 and ## p < 0.01 compared with the CON group. * p < 0.05 and ** p < 0.01 compared with the HF group.

PMC9180950

ijms-23-06263-g002.jpg

0.407586

3f6ce437b1e445a39e6e302cef7523ce

Hepatic genes expression involved in iron metabolism. (a) Hepatic mRNA expression of iron metabolism (n = 6). (b) Protein content of ZIP14 in the liver (n = 6). (c) Protein content of DMT1 in the liver (n = 6). (d) Protein content of FTL in the liver (n = 6). (e) Protein content of FPN in the liver (n = 6). (f) Hepatic mRNA expression of HAMP and the upstream regulators (n = 6). Values are means ± SEM. # p < 0.05 and ## p < 0.01 compared with the CON group. * p < 0.05 and ** p < 0.01 compared with the HF group.

PMC9180950

ijms-23-06263-g003.jpg

0.421934

b1c8e92d4dfa4d5f9a406429e582b200

Hepatic genes expression involved in methyl-transfer. (a) Hepatic mRNA expression of BHMT (n = 6). (b) Hepatic mRNA expression of GNMT (n = 6). (c) Hepatic mRNA expression of DNMT1 (n = 6). (d) Protein content of BHMT in the liver (n = 6). (e) Protein content of GNMT in the liver (n = 6). (f) Protein content of DNMT1 in the liver (n = 6). Values are means ± SEM. # p < 0.05 and ## p < 0.01 compared with the CON group. * p < 0.05 and ** p < 0.01 compared with the HF group.

PMC9180950

ijms-23-06263-g004.jpg

0.435726

48fbdd47f3fc4951b980db2e69b10655

Hepatic methylation level on promoter of affected genes. (a) Methylation status on the promoter of CD36 gene (n = 3). (b) Methylation status on the promoter of PPARγ gene (n = 3). (c) Methylation status on the promoter of HAMP gene (n = 3). (d) Methylation status on the promoter of BMP2 gene (n = 3). Values are means ± SEM. # p < 0.05 and ## p < 0.01 compared with the CON group. * p < 0.05 and ** p < 0.01 compared with the HF group.

PMC9180950

ijms-23-06263-g005.jpg

0.390848

8f80a0fb196e4e2ca49b5d59afaf62a6

Overview of the hypothesis and validation of anticipatory responses in E. coli MG1655 for carbon sources that have spatial abundances in the mammalian intestine.

PMC9181292

ijms-23-05985-g001.jpg

0.42056

aadeb3f6000e460eb4ae44187ddb2aa0

(a) The spatial abundance of different carbon sources in the intestine; (b) Expected and validated cross-regulation in the intestine. Top left slice, regulation based on the expectation and published knowledge; top right slice, regulation denoted from the RNA-Seq; bottom slice, regulation verified by the RT-PCR. Green, positive regulation; red, negative regulation; grey, no regulation; white, not measured.

PMC9181292

ijms-23-05985-g002.jpg

0.3938

69080fc3288b4cc89f84f875c883113a

Screening of E. coli MG1655 mutants demonstrating a positive association between D-galactose treatment and the expression of malP (a gene of the maltose operon). (a,b) Screening of D-galactose and D-maltose responsive E. coli strains; (c) Further characterization of demonstrating a positive association between D-galactose treatment and the expression of malP, at least in triplicate. The responses reported in plot A were measured in a plate reader, and the rest of the measurements were taken using a flow cytometer. * indicates significant differences (p-value < 0.05), and ns indicates no significant differences (p-value > 0.05). The p values were calculated by the two-sample t-test. Error bars indicate the standard error of the mean (±).

PMC9181292

ijms-23-05985-g003.jpg

0.463336

bf0dc0a6edf4493eb1789b6b9067961b

Identification of mutations and characterization of repair mutants. (a) Mutations in strains demonstrating the upregulation of malP expression in D-galactose supplemented media. EGK5_A8 and EGK8_A7 had point mutations in the coding region of crP, while EGK6_D3 had a point mutation in the promoter region of the malt gene; (b,c) Responses of repair mutants in 0.1% glycerol M9 and 0.1% glycerol M9 supplemented with 10 mM D-galactose. Fold repression was calculated by dividing the gfp (malP) levels in 0.1% glycerol M9 supplemented with 10 mM D-galactose with the gfp (malP) levels in 0.1% glycerol M9. Point mutations in EGK5_A8, EGK8_A7 and EG6_D3 were repaired to generate the repair mutants MING_crP2, MING_crP1 and MING_pmalT1, respectively. * indicates significant differences (p-value < 0.05). The p values were calculated by the two-sample t-test. Error bars indicate the standard error of the mean (±).

PMC9181292

ijms-23-05985-g004.jpg

0.452053

7e0bba1af8a34118a3739e3204d5691f

Competition assays to measure the fitness of lacZ deleted mutant strains showing a positive association between D-galactose treatment and malP expression. (a) Experimental strategy; (b) Fitness of lacZ deleted mutant strains against wild type strain MING.

PMC9181292

ijms-23-05985-g005.jpg

0.407597

c153bf7654cd4bd9b526323ee57a97e4

Fluorescence microscopy studies showing the ability of this technique to delineate the preferential localization of photosensitizing agents.

PMC9181573

ijms-23-06195-g001.jpg

0.487773

f86014e13b0140a69ae8d32e3a7069e0

Photokilling in 3D culture showing the synergistic effect of simultaneously targeting mitochondria and ER with benzoporphyrin derivative (BPD). Free BPD targets mitochondria > ER; ‘anchored’ BPD targets lysosomes. See Ref. [33] for further details.

PMC9181573

ijms-23-06195-g002.jpg

0.451701

4ad37a55a42449c48848e216c545a751

Morphology of apoptosis vs. paraptosis. Phase contrast images are compared with fluorescence-labeling of nuclei with Höchst 33342 [37].

PMC9181573

ijms-23-06195-g003.jpg

0.442121

9e2f915dda844b8aa4bf5233538e6f8c

Particle size distributions of IOT, PS, and SS.

PMC9181601

materials-15-03866-g001.jpg

0.497782

a26174d15e3545daa02372453ba467eb

SEM images of (a) IOTs, (b)PS, and (c)SS.

PMC9181601

materials-15-03866-g002.jpg

0.473069

dab891b8307245a896d417f221104340

The XRD pattern of the IOT.

PMC9181601

materials-15-03866-g003.jpg

0.424826

30da202c26d14a4d9547bd211c7b574c

The influence of (a) SCMs Type, (b) Content of IOT, (c) IOT Grinding Time, and (d) SCMs Content on the compressive strength of concrete.

PMC9181601

materials-15-03866-g004.jpg

0.436247

1f15dc0f7e5546a0a5d2c7d876160da8

Particle size distribution and cumulative sieve allowance of IOTs at different grinding times.

PMC9181601

materials-15-03866-g005.jpg

0.428327

7e3b79a9ed544c0db69616d5c225a0d5

SEM images of (a) Grinding IOTs for 0 h, (b) Grinding IOTs for 1.5 h, (c) Grinding IOTs for 2 h, and (d) Grinding IOTs for 2 h.

PMC9181601

materials-15-03866-g006.jpg

0.479863

f5de3c368ea04214a9224106a4b2bb9a

XPS patterns of IOTs at different grinding times of (a) binding energy of Si2p, (b) binding energy of Al2p, and (c) binding energy of Ca2p.

PMC9181601

materials-15-03866-g007.jpg

0.526773

6ed8738dd78e4f249bf863c90ec5bf90

XRD patterns of IOTs at different grinding times.

PMC9181601

materials-15-03866-g008.jpg