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0.42873 | b0bc7968a06e4f8bbaa3f6ac8b72a418 | Functional annotation of prokaryotic taxa (FAPROTAX) analysis revealing the different functions in (a) June, August, and September, and (b) upstream and downstream sections. | PMC9603554 | ijerph-19-13056-g004.jpg |
0.371652 | d7cbffcaa0af41b1b2f6ac97fca8eaa7 | Canonical correspondence analysis (CCA) plots showing the relationship between environmental factors and microbial communities. Factors identified as significantly influencing bacterial communities are highlighted in red. Each point represents a single sample (n = 24). Jun, Aug, and Sep represent the sample collection months June, August, and September, respectively. T, water temperature; ORP, oxidation–reduction potential; EC, electrical conductivity; DO, dissolved oxygen; Tur, turbidity; COD, chemical oxygen demand; TN, total nitrogen; TP, total phosphorus; NH4+-N, ammonia nitrogen; NO3−-N, nitrate nitrogen; Chl-a, chlorophyll-a. | PMC9603554 | ijerph-19-13056-g005.jpg |
0.449821 | d544730c7ec24522b862cd8a3a0c35d7 | Conceptual framework. | PMC9603680 | ijerph-19-13081-g001.jpg |
0.465406 | 7d481f40c0304749a1156d9d1c170939 | Moderating effect of relocation frequency. | PMC9603680 | ijerph-19-13081-g002.jpg |
0.537836 | 00cae5cd42eb469cb0802d6591175ff2 | Moderating effect of residential location. Note: Solid lines represent statistical significance at the 90% confidence interval, while dashed lines do not. | PMC9603680 | ijerph-19-13081-g003.jpg |
0.389987 | d01ea6224fea498aa7c3d1a428b09261 | Accumulated exposure to residential environment and subjective wellbeing in later life. Note: Solid circles represent statistical significance at the 90% confidence interval, while hollow circles do not. | PMC9603680 | ijerph-19-13081-g004.jpg |
0.455886 | 07ca9f2411f241049c697a4b834f66e8 | Phylogenetic relationships of the 28 CrHsfs from Canavalia rosea, the 38 GmHsfs from soybean (Glycine max), the 22 CaHsfs from chickpea (Cicer arietinum), the 24 VrHsf from mung bean (Vigna radiata), the 25 ZmHsfs from maize (Zea mays), and the 21 AtHsfs from Arabidopsis (Arabidopsis thaliana). The phylogenetic tree is constructed using MEGA 6.0 software with the Hsf protein sequences, by ClustalW alignment, the neighbor-joining (NJ) method, the bootstrap method, and 1000 repetitions. All subclasses (A1–A9, B1–B5, and C) of Hsf proteins are well separated in different clades and represented by different color backgrounds. | PMC9604225 | ijms-23-12357-g001.jpg |
0.406628 | 405a55c0ba594d15ba52bd2878246c36 | The motif composition predicted by the MEME prediction (http://meme-suite.org/, accessed on 1 May 2022) (A) and the conserved domain diagram drawn manually (B) of C. rosea Hsf proteins. The far left indicates the constructed phylogenetic tree with MEGA 6.0, including group A (A1, A2, A4, A5, A6, A7, A8, A9), group B (B1, B2, B3, B4, B5), and group C. The ten conserved motifs are listed in the left bottom box, and the four functional domains, including DBD, OD, AHA, and RD, are listed in the right bottom box. | PMC9604225 | ijms-23-12357-g002.jpg |
0.474982 | 4ecb674f048a4b63afd6e5bab5484eac | (A) Chromosomal distribution of 28 CrHsf genes in the C. rosea genome. The scale of the chromosome is showed in millions of bases (Mb); (B) the distribution of segmental duplication of CrHsfs in C. rosea chromosomes. | PMC9604225 | ijms-23-12357-g003.jpg |
0.436953 | ac9b40821c6645d2b8a0d1f1aa272d73 | The exon-intron organization of the CrHsf genes constructed with GSDS 2.0 (http://gsds.cbi.pku.edu.cn/, accessed on 1 May 2022). The left capital letters “A, B, and C” and corresponding phylogenetic tree represent different groups marked with different colors (red for Group A, yellow for Group B, and blue for Group C). | PMC9604225 | ijms-23-12357-g004.jpg |
0.418912 | 7ffbf736c1794b64899281adc0ae919a | Prediction of cis-regulatory elements in the 2000 bp upstream regulatory regions of CrHsf genes: (A) Summaries of the thirteen cis-regulatory elements in the 28 CrHsf promoter regions; (B) distribution of the five cis-regulatory elements (ABRE, MYC, MYB, TC-rich repeat, and HSE) in the CrHsf genes promoter regions. The scale bar represents 200 bp. | PMC9604225 | ijms-23-12357-g005.jpg |
0.461148 | 665dc2d87ef544a8b1ddc8b40b859057 | Heatmaps showing: (A) The expression levels of the 28 CrHsf genes in the root, stem, leaf, flower bud, and young fruit of C. rosea plant; (B) expression differences of the 28 CrHsf genes in mature C. rosea leaves captured from adult plants growing in the South China Botanical Garden (SCBG) and on Yongxing Island (YX). The expression level of each gene is shown in FPKM values (log2). Orange red denotes high expression levels, and dark blue denotes low expression levels. | PMC9604225 | ijms-23-12357-g006.jpg |
0.422145 | 0598853752114710ac68d96d45f114c0 | Heatmaps showing: (A) The expression levels of the 28 CrHsf genes in roots and leaves of C. rosea seedlings under heat shock stress (45 °C for 2 h); the expression patterns of the 28 CrHsf genes under abiotic stress treatment (600 mM NaCl, 150 mM NaHCO3, pH 8.2, 300 mM mannitol, for 2 h and 48 h) in roots (B) and leaves (C). The expression level of each gene is shown in FPKM values (log2). Orange red denotes high expression levels, and dark blue denotes low expression levels. | PMC9604225 | ijms-23-12357-g007.jpg |
0.436287 | 3f0badcfab164b248abe4f285299960e | Tempo-spatial expression analysis of the 10 CrHsf genes with quantitative reverse transcription PCR (qRT-PCR) responding to different stresses: (A) The relative expression patterns of 10 CrHsf genes under salt stress (150 mM NaCl); (B)alkaline stress (NaHCO3); (C) high osmotic stress (300 mM mannitol); (D) heat stress (45 °C), in C. rosea seedling plants. Relative expression values were calculated using the 2−ΔCt method with the housekeeping gene CrEF-1α as the reference gene. | PMC9604225 | ijms-23-12357-g008.jpg |
0.502439 | 6e9269fb02154f3bbe19b071805427d6 | Transactivation assay of the eight CrHsf proteins in yeast cells. The GAL4 DNA binding domain was fused with eight CrHsfs and transformed into the yeast strain AH109 containing the His3 and LacZ reporter genes. An analysis of β-galactosidase activity of the relative yeast strains on plates was also performed. The yeast culture (OD600 to 2) was serially diluted to OD600 values of 0.2, 0.02, and 0.002, and then the 2 μL yeast liquid was spotted onto the SD plates and cultured for 2 d at 30 °C. Negative control, yeast cells transformed with pGBKT7 empty vector; positive control, yeast cells transformed with CrASR1-pGBKT7 [25]. Experiments were performed three times with similar results. | PMC9604225 | ijms-23-12357-g009.jpg |
0.460461 | b612de9731354fe2afe14b901c0deddc | The dual-luciferase assay of the eight CrHsf proteins on synthetic reporter plasmids (4 × GAAACTTC (HSE)-pGreenII0800-LUC or 4 × GACACACT (mHSE)-pGreenII0800-LUC): (A) The schematic diagram of the constructs. REN, Renilla luciferase; LUC, firefly luciferase; terM, terminator; (B) the ratio of LUC/REN activity in Nicotiana benthamiana mesophyll cells co-transfected with agrobacterium GV3101 containing different LUC reporters (HSE or mHSE) and REN effectors (different CrHsfs and pBIm for testing or empty vector pBIm as negative control). ** The values are means ± SD of three biological replicates. | PMC9604225 | ijms-23-12357-g010.jpg |
0.393002 | 6436b1b2056842b9a6d8c8d44e1d65cc | The tolerance confirmations of the eight CrHsf gene heteroexpression in yeast. H2O2 oxidative stress and heat stress (52 °C 15 min) tolerance confirmations in yeast mutant strain skn7∆ (A) and yap1∆ (B). The stress factors with different concentrations are shown in the figures. Yeast cultures were adjusted to OD600 = 2, and 2 μL of serial dilutions (10-fold, from left to right in each panel) was spotted on SDG/-Ura medium supplemented with different concentrations of H2O2 (0.4 mM, 0.5 mM, and 0.6 mM), and small portions of the yeast cultures were incubated at 52 °C for 15 min, and then were moved to a 30 °C environment before being spotted on SDG/-Ura medium (without any chemical stress factors) for the thermotolerance confirmation. Corresponding yeast spots growing on SDG/-Ura plates without H2O2 or heat stress were used as the control. The plates were incubated for 2–5 days at 30 °C. The images are representative of three independent experiments. | PMC9604225 | ijms-23-12357-g011.jpg |
0.553216 | 3d493e2692d04c049ac0650dd53e53a4 | The heat stress tolerance confirmation (52 °C 30 min) of the eight CrHsf genes heteroexpression in yeast wild type strain (WT). Yeast cultures were adjusted to OD600 = 2, and small portions of the yeast cultures were incubated at 52 °C for 30 min, and then were moved to a 30 °C environment before being spotted on SDG/-Ura medium (without any chemical stress factors) for the thermotolerance confirmation. The corresponding yeast spots growing on SDG/-Ura plates without heat stress were used as the control. The plates were incubated for 2–5 days at 30 °C. The images are representative of three independent experiments. | PMC9604225 | ijms-23-12357-g012.jpg |
0.468792 | 3505f6f4751a41eb9e365c154ce2ea87 | Two-step diagnosis for sepsis-associated DIC. The figure depicts an algorithm to diagnose sepsis-induced coagulopathy (SIC) and overt disseminated intravascular coagulation (DIC). Sepsis patients with thrombocytopenia (platelet count < 150 × 109 × L−1) are screened by using SIC diagnostic criteria (Step 1), and then by using overt DIC diagnostic criteria (Step 2). The rationale for this approach is that SIC and overt DIC represent a continuum wherein the onset of SIC typically precedes that of overt DIC, and where early therapeutic intervention with anticoagulant therapy is most likely to be beneficial. Adapted with permission from Ref. [12]. Copyright ©2019, Blackwell Publishing. | PMC9604230 | ijms-23-12474-g001.jpg |
0.467844 | e3f97484ece9407b863b792168f36464 | Functions and effects of antithrombin in host defense and inflammation. Note: AT, antithrombin; LPS, lipopolysaccharide; HSPG, heparan sulfate proteoglycans; GAG, glycosaminoglycans. Reproduced without modification from Schlömmer et al. 2021 [50] (accessed on 24 September 2022), under a Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International (CC BY-NC-ND 4.0) License (https://creativecommons.org/licenses/by-nc-nd/4.0/ (accessed on 24 September 2022). Copyright © 2021, The Authors. This reuse has not been endorsed by the licensor. The source reference is “[50]” and is available at https://www.mdpi.com/1422-0067/22/8/4283. | PMC9604230 | ijms-23-12474-g002.jpg |
0.535904 | 193bc7e61ef94cf188b530534f9d83dc | Possible mechanisms for thrombosis in coronavirus disease 2019 (COVID-19) and clinical consequences. (A) Injury to the endothelium initiated by severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) entry into cells via the angiotensin-converting enzyme 2 (ACE2) receptor is thought to lead to diffuse endotheliitis. The endothelial damage may result in an inflammatory host response characterized by excessive immune activation and cytokine storm, which promotes hypercoagulability and thrombosis. (B) Possible venous and arterial thrombotic complications associated with COVID-19. Original illustration by freelance medical illustrator Gail Rudakevich. Reprinted with permission from Ref. [15]. Copyright ©2021, Canadian Medical Association. Abbreviations: DVT, deep vein thrombosis; FVIIa, factor VIIA; IL-6, interleukin 6; PE, pulmonary embolism; TF, tissue factor; TNF, tumor necrosis factor α. | PMC9604230 | ijms-23-12474-g003.jpg |
0.516307 | edf27b1ccf8a44b98b8ae6f37c7dfee8 | Comparison of the placental CLN4 expression in pregnancy complicated by fetal growth restriction with brain-sparing regarding the occurrence of an intraventricular hemorrhage in newborns. | PMC9604432 | ijms-23-12349-g001.jpg |
0.384578 | b84093d497e842e5b645440f01bbb959 | Generation of pre-vascularized collagen sponges. (A) In vitro experimental design. (B) Representative macroscopic images of shark skin collagen sponge’s macroporosity after freeze drying. Scale bar: 1000 µm. (C) Representative immunocytochemistry images of the network-like organization of SVF-derived CD31-expressing cells (white) interconnected with pericytes CD146-expressing cells (green), within collagen sponges after 7 days of culture in the absence of extrinsic angiogenic growth factors. Cell nuclei were counterstained with DAPI (blue). Scale bar: 75 μm (top) and 25 µm (bottom). (D) Angiogenic secretome profile of SVF cells seeded in collagen sponges at different culture periods. Conditioned media were collected at days 5 and 7 for dot blot analysis of angiogenesis-related factors. Protein expression profiles were measured using mean intensity and normalized to the reference spots. Data are presented as mean ± std dev and were analyzed using a paired t-test (* p < 0.0332, ** p < 0.0021, *** p < 0.0002, and **** p <0.0001). | PMC9604698 | marinedrugs-20-00623-g001.jpg |
0.469047 | 38ce7991f7c54c55ac3ebbacccfbcd89 | In ovo angiogenic potential upon implantation in Chick Chorioallantoic Membrane (CAM). (A) In vivo experimental design. (B) Representative micrographs of recruited vessels after 4 days of implantation of collagen sponges with and without SVF. Scale bar: 2000 µm. (C) Quantification of recruited vessels after 4 days of implantation of collagen sponge with and without SVF. Data are presented as violin plot illustrating the kernel density distribution frequency of recruited vessels and analyzed using an unpaired t-test (** p < 0.0021). (D) Representative micrographs of hematoxylin and eosin staining in collagen sponges with and without SVF. Scale bar: 500 μm (left) and 50 μm (right). | PMC9604698 | marinedrugs-20-00623-g002.jpg |
0.409523 | 497f9cf9f45f46b08c92e158746d9641 | In ovo angiogenic potential upon implantation in Chick Chorioallantoic Membrane (CAM). (A) Representative images of the in situ hybridization performed with a DNA probe that stains human cellular nuclei (blue, arrows) in contrast with chicken nuclei (pink). The implanted cells infiltrated the host tissue and vasculature as highlighted by black arrows. Chicken erythrocytes identified by orange arrows. Scale bars: 200 μm (left) and 50 μm (right). (B) Representative immunohistochemistry images of the collagen sponge after 4 days of implantation showing human CD31-positive cells (brown). Human CD31 expression patterns demonstrated the integration of the pre-vascular network in the CAM, as highlighted by black arrows. Chicken erythrocytes identified by orange arrows. Scale bars: 500 μm (left) and 20 μm (right) and 50 µm (inset right). | PMC9604698 | marinedrugs-20-00623-g003.jpg |
0.492862 | a714ea8f3f004f65ae2bb6ce40227dfb | Map showing the sampling locations of Pleurotus spp. collection in and around Rajaji National Park at Haridwar, Uttarakhand, India (Source: Google Earth Pro). | PMC9605409 | jof-08-01007-g001.jpg |
0.526986 | 9b19024db2c346199cdd8811fa4e6e31 | (a) Axial biplot; (b) PCA-standardized scores; and (c) HCA dendrogram with heatmap for heavy metal contents in P. ostreatus collected from different locations of Rajaji National Park, Haridwar, India. | PMC9605409 | jof-08-01007-g002.jpg |
0.484527 | fd4c1ab3063445f98a24f5e01af3c6d1 | (a) Axial biplot; (b) PCA-standardized scores and (c) HCA dendrogram with heatmap for heavy metal contents in P. djamor collected from different locations of Rajaji National Park, Haridwar, India. | PMC9605409 | jof-08-01007-g003.jpg |
0.517165 | e0dca055d11542dead0cb5d92cf6df36 | Spider-web diagram showing the health risk index (HRI) values of heavy metal contents in two oyster mushroom spp. (PO: P. ostreatus and PD: P. djamor) collected from different locations of Rajaji National Park, Haridwar, India. | PMC9605409 | jof-08-01007-g004.jpg |
0.492771 | 8aaff00f42b04c42aac4bbbdceeceada | Phylogenetic tree of the studies insects for the genome selection. All nodes were 100% bootstrap supported. N. vitripennis was used to root the tree. For A. mellifera carnica, 93.53% of complete BUSCOs were found, which suggests the assembly is complete. | PMC9605442 | life-12-01642-g001.jpg |
0.392444 | cbb9771992bd4b89862f5eb988f0d2b2 | Coverage map of the honeybee subspecies. The genomes of A. mellifera melllifera and A. mellifera DH4 were compared with A. mellifera carnica. Overall, 80% of the nucleotides were aligned. | PMC9605442 | life-12-01642-g002.jpg |
0.436444 | 205bad1e1ca64fd68736d9cdeccbf237 | Microsatellite analysis of the three honeybee subspecies. (A): the distribution of microsatellites of the dinucleotide motif. The number of microsatellites decreased with the increasing number of repeats for all three genomes. Overall, the variation of the microsatellite distribution among the three genomes was not significant. (B): Venn diagram of the linkage map makers among the three honeybee genomes. A. mellifera DH4 shared significantly higher number of markers with A. mellifera carnica compared with A. mellifera mellifera. | PMC9605442 | life-12-01642-g003.jpg |
0.437794 | 876f47fa0a144422a89f576fc3c6fbab | Phylogenetic tree of social genes. (A): phylogenetic tree of hexamerin family proteins. (B): phylogenetic tree of insulin family. The orthologs were retrieved by BLAST in NCBI manually with E-value cutoff ≤ 1 × 10−5. The sequences were aligned with Muscle and the phylogenetic tree was constructed using Neighbor joining model with 1000 bootstrap. | PMC9605442 | life-12-01642-g004.jpg |
0.448253 | faa9f2f9ddd74279ba057a7409563b95 | Functional categories significantly enriched of genes under positive selection in the branch A. m. ligustica, using REVIGO to cluster the long list of significant GO terms. The medium cluster size was 0.7 and the semantic similarity measure SimRel. | PMC9605442 | life-12-01642-g005.jpg |
0.400127 | 2059bfef7e6746be96b6a3fd56a89d1b | Functional categories significantly enriched of genes under positive selection in the branch A. mellifera spp. | PMC9605442 | life-12-01642-g006.jpg |
0.404776 | 0d4758d0bef6435191a0e358c0ef495a | (a) Effects of MS with different plant growth promoters on shoot length, (b) root length, (c) number of leaves/plant, and (d) leaf area in milk thistle in 2017 and 2018. Different letters represent significant differences at the p < 0.05. | PMC9605483 | life-12-01530-g001.jpg |
0.385628 | 4ff0bbacf0f24028a033f1e347ba8b33 | (a) Effects of MS with different plant growth promoters on shoot fresh weight, (b) root fresh weight, (c) shoot dry weight, and (d) root dry weight in milk thistle in 2017 and 2018. Different letters represent significant differences at the p < 0.05. | PMC9605483 | life-12-01530-g002.jpg |
0.407611 | ab41fa983510427d816b096c2acd220c | (a) Effects of MS with different plant growth promoters on number of roots/plant, (b) number of spines/leaf, (c) root diameter, and (d) relative water content in milk thistle in 2017 and 2018. Different letters represent significant differences at the p < 0.05. | PMC9605483 | life-12-01530-g003.jpg |
0.434407 | a1b6c9fa61ee436c81b06b5a6110c008 | (a) Effects of MS with different plant growth promoters on shoot saponin contents and (b) root saponin contents in milk thistle in 2017 and 2018. Different letters represent significant differences at the p < 0.05. | PMC9605483 | life-12-01530-g004.jpg |
0.446644 | b52e57b087ff48e1892368eb5d4cbe6e | (a) Effects of MS with different plant growth promoters on shoot anthocyanin contents and (b) root anthocyanin contents in milk thistle in 2017 and 2018. Different letters represent significant differences at the p < 0.05. | PMC9605483 | life-12-01530-g005.jpg |
0.453792 | 007d1d0f376843f0a1d74e0597ae4371 | (a) Effects of MS with different plant growth promoters on shoot alkaloids contents and (b) root alkaloids contents in milk thistle in 2017 and 2018. Different letters represent significant differences at the p < 0.05. | PMC9605483 | life-12-01530-g006.jpg |
0.403252 | d333b751a98945b8831d39c99165e918 | Principal component biplot of three ecotypes for all the traits under study in years (a) 2017 and (b) 2018. RFW = root fresh weight; SFW = shoot fresh weight; RDW = root dry weight; SDW = shoot dry weight; RAl = root alkaloids; SAl = shoot alkaloids; RAn = root anthocyanins; SAn = shoot anthocyanins; RS = root saponins; SS = shoot saponins; RWC = relative water content. | PMC9605483 | life-12-01530-g007.jpg |
0.584187 | f23a0bcb65e04af891e422506d731fdb | Sarcsteroid F (1) and 24-methylenecholestane-1α, 3β, 5α, 6βe, 11α-pentol-11-monoacetate (2) isolated from the LME. | PMC9605502 | life-12-01470-g001.jpg |
0.436739 | b1c41cf1134f48d195bf194fd9d0cfa2 | 1H NMR spectrum of compound 1 measured in CD3OD (600 MHz). | PMC9605502 | life-12-01470-g002.jpg |
0.437664 | 264144983ac243abbf5bd428bfcd6244 | Expanded 1H NMR spectrum of compound 1 measured in CD3OD (600 MHz). | PMC9605502 | life-12-01470-g003.jpg |
0.448223 | 48cf4535c5eb46759e15e032cc7c4348 | 13C NMR spectrum of compound 1 measured in CD3OD (150 MHz). | PMC9605502 | life-12-01470-g004.jpg |
0.459947 | 03300f05521d408eaf41db3196e1f8d0 | Expanded 13C NMR spectrum of compound 1 measured in CD3OD (150 MHz). | PMC9605502 | life-12-01470-g005.jpg |
0.479475 | 6be80b76d66e4f518e5ee7fc2f0c4bb2 | HSQC spectrum of compound 1 measured in CD3OD (150 MHz). | PMC9605502 | life-12-01470-g006.jpg |
0.395484 | 8f4b98d1737c413798ea60d16abe582e | HRESIMS spectrum of compound 1. | PMC9605502 | life-12-01470-g007.jpg |
0.423982 | e34487f5888b497da4fb3538d946e663 | 1H NMR spectrum of compound 2 measured in CD3OD (600 MHz). | PMC9605502 | life-12-01470-g008.jpg |
0.41602 | 1475f53b7aa84dff9144f8beb96bd090 | Expanded 1H NMR spectrum of compound 2 measured in CD3OD (600 MHz). | PMC9605502 | life-12-01470-g009.jpg |
0.426094 | a28f555e86004c18babb82a741329da1 | 13C NMR spectrum of compound 2 measured in CD3OD (150 MHz). | PMC9605502 | life-12-01470-g010.jpg |
0.401705 | 651caa33516748d9b4177b901e965297 | Expanded 13C NMR spectrum of compound 2 measured in CD3OD (150 MHz). | PMC9605502 | life-12-01470-g011.jpg |
0.477856 | dcb5894939d1455692c751cc08588503 | HSQC spectrum of compound 2 measured in CD3OD (150 MHz). | PMC9605502 | life-12-01470-g012.jpg |
0.475113 | ca81c9fed98949b188e15fd6b64171d8 | HRESIMS spectrum of compound 2. | PMC9605502 | life-12-01470-g013.jpg |
0.505964 | 1218a51b63da43d18181eb775f92fbb3 | Effect of colon cancer and colon cancer-LME treated rats on serum level of (a) CA19.9, (b) CEA, (c) AFP, (d) TNF-α, (e) IL-1β, (f) CD4+, as well as (g) DNA fragmentation percentage. Symbol (*) is significantly different from the control group; symbol (#) is significantly different from DMH group at p ≤ 0.05 level; DMH is dimethylhydrazine; LME is Litophyton sp. methanolic extract. | PMC9605502 | life-12-01470-g014a.jpg |
0.444276 | 5447675aeedd479497997b1bf1113907 | Photomicrographs of colon sections stained with H&E. (a) a section of normal rat colon showed normal colonic mucosa consisting of straight crypts with no villi; (b) a colon section of normal rat injected with LME only, showing the normal histologic structure, (c) a colon section of cancer modeled rats showed rupture of crypts, and huge infiltration of lymphocyte, and eosinophils were observed. In addition, adenomatous polyp was characteristic for this group; (d) colon section of cancer-modeled rats treated with LME showing regenerated colon architecture with just mucosal ulceration. | PMC9605502 | life-12-01470-g015.jpg |
0.421992 | 53bca9810f4645bbb2646abd2dce88ff | Geographical location of the buildings for which the mycobiome was characterized, in black for those for which molecular and cultivable data were obtained, and in red for those for which only the culture provided results. Their belonging to an environmental typology is indicated by a triangle for peri-urban cluster, by a circle for the suburban cluster, by a plus for the historic downtown cluster, by a diamond for the lakefront downtown, and by a square for the lakefront. Elevation lines every 100 m and 20 m are indicated with thick and thin brown lines, respectively. | PMC9605656 | jof-08-01045-g001.jpg |
0.416334 | 6ec934327384455d9b0217611975630e | Visualization of the similarity in genus composition of fungal communities across the studied buildings. Each point represents the fungal community in a building, such that those that are closer together share more identified genera in common than those farther apart. Fungal community composition and relative abundance of genera tend to cluster according to season (a), period of building construction (b), or proximity to green space in the surroundings (c). | PMC9605656 | jof-08-01045-g002.jpg |
0.419738 | 1df6d8ab1a06453e9575a912571dc7da | Boxplots showing distribution in relative abundance of the nine most abundant and frequent genera across samples. The values observed during the heating period are shown in red, and those during the unheated period are shown in blue. The whiskers in a box-and-whisker plot are the adjacent values that correspond to the highest value not greater than p75 + 3/2 IQR and the lowest value not less than p25 − 3/2 IQR, where IQR is the inter-quartile range, the box covers the values between the first and third quartile, and the line in the box marks the median value. | PMC9605656 | jof-08-01045-g003.jpg |
0.431029 | d07d258ad7ba48a1b82b3dbd82d5d3ea | Visualization of the proportion (a) and log transformed mean relative abundance (b) of indoor fungal genera that differed significantly between the unheated and heating periods (p < 0.05). Values observed during the heating period are shown in red, and those observed during the unheated period are in blue. | PMC9605656 | jof-08-01045-g004a.jpg |
0.37972 | 669fd9d9ad4742db8874ba6baa1b79f3 | Visualization of the proportion (a) and log transformed mean relative abundance (b) of indoor fungal genera that differed significantly between buildings constructed at different periods (p < 0.05). Shown in grey are taxa observed in buildings constructed before 1944, in red are those observed in buildings constructed between 1945 and 1974, and in yellow are taxa observed in buildings constructed between 1975 and 2014. | PMC9605656 | jof-08-01045-g005.jpg |
0.424915 | a98c4edc7f7d4598920110fc534ec933 | Visualization of the proportion (a) and mean log transformed relative abundance (c,d) of indoor fungal genera that differed significantly between buildings with and without green space in the surroundings (p < 0.05)—(c) during the unheated period (d) during the heating period. The distribution of relative abundance among samples is illustrated for few taxa in (b). In green, values for buildings with green space in the surroundings, and in black, values for buildings without green space in the surroundings. | PMC9605656 | jof-08-01045-g006a.jpg |
0.431073 | 43fa0c7a1d2e4362926ea26c8aad2993 | An overview of tissue culture process (A, B) small explant develops callus which then produces shoots a few weeks after being placed into tissue culture media (C) “A to I” shows complete procedure from single cell placement to MS media to development of a complete plant (D) How all phases in plant tissue culture from initiation, multiplication, root formation, shoot formation and acclimatization occurs. | PMC9606719 | fpls-13-1009395-g001.jpg |
0.435319 | 5627a43a566349218655c2763dd48f8b |
In vitro plant propagation of plants at Tissue Culture Lab (A, B) Roots are fully developed prior to moving plants to pots of soil. | PMC9606719 | fpls-13-1009395-g002.jpg |
0.460647 | 61015ff34df648a6ac2f1e3976a68597 | Recent methods used for industrial production of bioactive compounds via plant tissue culture. | PMC9606719 | fpls-13-1009395-g003.jpg |
0.416201 | 39f3a1be887f4d3d8b6e9832df0f5fe3 |
In vitro propagation of banana (A) Callus formation to roots development (B) Maturation of plants in media (C) Plant sown in pot (D) Plant sown in soil in controlled conditions, acclimatization (E) Sowing of plant in the field (F) Tissue culture produced Banana field. | PMC9606719 | fpls-13-1009395-g004.jpg |
0.424103 | bfa005a6b6764af2a48ade1d112a2d3f |
In vitro propagation of pineapple (A, B) Initiation and multiplication stage (C–E) Root and shoot formation, plants are fully grown to move to pots of soil. | PMC9606719 | fpls-13-1009395-g005.jpg |
0.443716 | 3db0b867776a4a92b92d95a077e63673 | Wheat tissue culture (A) Inoculation of single cell to tissue culture media (B) Root and shoot development (C, D) Roots are fully developed prior to moving plants to pots of soil. | PMC9606719 | fpls-13-1009395-g006.jpg |
0.42708 | 428fb024d1ee46a9b126847c272e2d4b | Rice tissue culture (A–C) Single cell to callus formation (D, E) Sub culturing and regenerated plants are ready to move to pots. | PMC9606719 | fpls-13-1009395-g007.jpg |
0.485595 | 1dadcd18ef4b4bf28fdd1ad3b635796b | Structures of (A) Berberine (B) Valepotriates (C) Taxol. | PMC9606719 | fpls-13-1009395-g008.jpg |
0.512601 | b6c0e8aa928c4bc5840deb09fd8f34d2 | Immunohistochemical staining for FKBPL in endometrioid endometrial carcinoma showing no expression—0 (A), low expression—1 (B), moderate expression—2 (C), and immunohistochemical staining for FKBPL in benign endometrial hyperplasia showing high expression—3 (D). Magnification ×200. | PMC9606853 | medicina-58-01330-g001.jpg |
0.458861 | d8090805bb1e49cfb9a8ab3a7847f263 | Immunohistochemical staining for ERα in endometrioid endometrial carcinoma showing low expression—1 (A), moderate expression—2 (B), high expression—3 (C), and immunohistochemical staining for ERα in benign endometrial hyperplasia showing high expression—3 (D). Magnification ×200. | PMC9606853 | medicina-58-01330-g002.jpg |
0.493088 | 459934b47a034f3da76c1828acc5ed95 | Immunohistochemical staining for VEGF-A in endometrioid endometrial carcinoma showing no expression—0 (A), low expression—1 (B), moderate expression—2 (C), and immunohistochemical staining for VEGF-A in benign endometrial hyperplasia showing no expression—0 (D). Magnification ×200. | PMC9606853 | medicina-58-01330-g003.jpg |
0.475914 | 79d6e7cb70134dd28b3b211752887aff | Peroxynitrite treatment of F. tularensis. (A) Modification of tyrosine residues by peroxynitrite (PN): the tyrosyl (phenol) group is nitrated, generating nitrotyrosine. (B) PN inactivation of F. tularensis: 5 × 109 CFU/mL F. tularensis was incubated with 5 mM or 10 mM PN for 15 min, or twice with 5 mM PN. As a control, bacteria were incubated with 60 mM of NaOH. The LOD for viable bacterial counts is 5 CFU/mL. (C) Western blot analysis of inactivated LVS cells. Lysates of inactivated LVS by formalin (formalin-killed, FK), peroxynitrite (PN), peroxynitrite-inactivated bacteria treated with dithionite (PN D), and untreated bacteria (LVS) were analyzed by a specific anti-NT antibody. Equal amounts (108 cell equivalents) of protein were loaded in each lane. | PMC9607248 | vaccines-10-01593-g001.jpg |
0.464908 | c5a67b212f85415dacb6e694a4f507c0 | Macrophage response to whole inactivated bacteria. J774 cells (A), U937 cells (B), and MH-s cells (C) were incubated with formalin (FK)- or peroxynitrite (PN)-neutralized F. tularensis LVS bacteria. Macrophage activation was quantified by TNFα secretion into the media. E. coli LPS (LPS) was used as a positive control; (*) p < 0.05, (**) p < 0.01. | PMC9607248 | vaccines-10-01593-g002.jpg |
0.443522 | d042a0d90e314d2b9312a6f2d1bb655d | Early dendritic cell (DC) maturation, following incubation with neutralized bacteria. BMDCs were incubated for four hours with formalin (FK) or PN-neutralized bacteria, as indicated in the inset legend, stained for maturation markers expression and analyzed by flow cytometry. (A) The data are presented as the percentage of mean fluorescence intensity (MFI) change compared to non-treated cells. LPS (100 ng/mL) served as a positive control for DC maturation. (B) Representative dot blot presentation of CD11c+ cells following 24 h of incubation with FK- (red) or PN- (blue) inactivated bacteria presenting each marker (horizontal axis) versus forward scatter. | PMC9607248 | vaccines-10-01593-g003.jpg |
0.464943 | cdd1cfe082f14de8ad0cb649561bf6e9 | T cell response to whole bacterial antigens inactivated by formalin or peroxynitrite. Splenocytes from immunized mice were incubated with formalin- or peroxynitrite (PN)-neutralized F. tularensis LVS bacteria for 24 h. T cell response was measured by an IFNγ ELISPOT test. (****) p < 0.0001. | PMC9607248 | vaccines-10-01593-g004.jpg |
0.471313 | feaffed97763435393c996eef72a92e9 | Survival of mice immunized by whole bacterial antigens inactivated by formalin (n = 14) or peroxynitrite (n = 14). Balb/c mice were immunized intranasal with 108 cfu/mL of formalin (FK)- or peroxynitrite (PN)-inactivated bacteria. Fourteen days post-immunization, mice were challenged intranasal with 10 LD50 (104 cfu/mL) of F. tularensis LVS. Survival was monitored for 21 days. Naïve mice (n = 10) were used as controls. (*) p < 0.05. | PMC9607248 | vaccines-10-01593-g005.jpg |
0.410905 | 7b7e2ae2f59c4be9be158a1f1e0ef19f | CRISPR-Cas9-based gene disruption all-in-one circular plasmid. | PMC9607276 | microorganisms-10-02088-g001.jpg |
0.495482 | 50c03dde61174405a96ba18086938d65 | Ascosphaera apis (A. apis) mycelial growth limiting concentration level of hygromycin B. (a) Graphical hygromycin B resistance level test of A. apis; (b) 0 µg/mL hygromycin B; (c) 10 µg/mL hygromycin B; (d) 25 µg/mL hygromycin B. | PMC9607276 | microorganisms-10-02088-g002.jpg |
0.505751 | 185271e8979441b9842d453ea4914cba | Expression of EGFP in transformed Ascosphaera apis (A. apis) mutant mycelia with plasmid containing EGFP and wild-strain group viewed using a confocal laser scanning microscope illuminated with a UV light. (a,b) Mycelia of transformed A. apis StcU-2 mutants expressing EGFP. (c,d) Mycelia of A. apis wild strain (non-transformed) without expressing EGFP. | PMC9607276 | microorganisms-10-02088-g003.jpg |
0.408997 | 5df14d89b1e5467089a266d9b23a2bd1 | Different amplified StcU-2-gene-edited Ascosphaera apis (A. apis) target gene bands. (a) Bands of wild-strain, eight stably selected Cas9-gene-edited StcU-2 mutants of A. apis, and negative control for EGFP gene integration. (b) Bands of wild-strain, eight stably selected Cas9-gene-edited StcU-2 mutants of A. apis, and negative control for hph gene integration. (c) Bands of wild-strain, eight stably selected Cas9-gene-edited StcU-2 mutants of A. apis, and negative control for StcU-2 gene being edited. For all: M denotes the DNA Marker 700 ladder; WT denotes A. apis wild strain. Lanes 1–8 denote different independent StcU-2 transformant mutants; AAStcU-2-1911-1, AAStcU-2-1911-2, AAStcU-2-1911-3, AAStcU-2-1911-4, AAStcU-2-1911-5, AAStcU-2-1911-6, AAStcU-2-1911-7, and AAStcU-2-1911-8. –Ve denotes the negative control, which is a mix of sterile, double-distilled water and PCR without a template. | PMC9607276 | microorganisms-10-02088-g004.jpg |
0.492728 | 9b53c261aedd43ac9e96db0821c9eb5a | Clustal W sequence alignment of StcU-2 gene-edited mutants. AA Wild-Strain denotes Ascosphaera apis (A. apis) wild-strain protospacer sequence and PAM sequence. AAStcU-2-1911-1, AAStcU-2-1911-2, AAStcU-2-1911-3 AAStcU-2-1911-4, AAStcU-2-1911-5, AAStcU-2-1911-7, and AAStcU-2-1911-8 denote different independent StcU-2 gene-edited mutant protospacer sequences and PAM sequence site editing. | PMC9607276 | microorganisms-10-02088-g005.jpg |
0.428113 | eacbb64e64f54124885dfccba8c02527 | Mating type compatibility test and effective sporulation of different Ascosphaera apis (A. apis) strain. (a) Sporeless fluffy mycelial growth structure of A. apis mutant colonies. (b) Matured and remarkably small sized A. apis mutants spore cysts. (c) Effective matured A. apis wild strain asco-spores. (d) Mating type compatibility and asco-spore development test among different Cas9 edited StcU-2 mutant A. apis colonies. Arrows show line of spore cyst barrage for StcU-2 mutants, and wild strain A. apis after mating. Numbers denote individual mutants. Black dots denote initial mycelial inoculation sites. (e) Effective mating and sporulation of wild strain. Arrows show line of spore-cyst barrage. | PMC9607276 | microorganisms-10-02088-g006.jpg |
0.430439 | ae479dd7d6854464bc5cf3301e8bf548 | The 600 bp single-plex PCR products for each of the thirteen primers pairs (600-1–600-13, lanes 1 to 13) used to generate overlapping amplicons for tiled amplicon sequencing for (A) VPRI43306 and (B) NSW3-35. The amplicons were visualised on 1.5% agarose gel with SYBR™ Safe DNA gel stain (Thermo Fisher Scientific, Scoresby, Australia). A 100 bp DNA ladder (Thermo Fisher Scientific Scoresby, Australia) was used for product size estimation. | PMC9607580 | plants-11-02716-g001.jpg |
0.466458 | 9f85bd15856e456a8c7be0462ed4b39a | Genome coverage and read depth profiles of plant panel tenfold dilution series samples, from undiluted to 1 in 100,000,000,000 (10−11) across the 6377 nt cucumber green mottle mosaic virus (CGMMV) genome (VPRI43306) detected using tiled amplicon nanopore sequencing. Each dilution was tested in triplicate and the results for each technical replicate are shown as different colours. Depth is in standard logarithmic scale. | PMC9607580 | plants-11-02716-g002.jpg |
0.469285 | f11404d25f4b4b9db5c697af61dc9553 | Genome coverage and read depth profiles of seed panel dilution series samples, from undiluted to 1 seed in 1000 across the 6377 nt reference cucumber green mottle mosaic virus (CGMMV) genome (NSW3-35) detected using tiled amplicon nanopore sequencing. Biological replicates are shown in each profile. Each biological replicate was tested in triplicate and the results for each technical replicate are shown as different colours. Depth is in standard logarithmic scale. | PMC9607580 | plants-11-02716-g003.jpg |
0.474021 | 6041cc916e2d4ae182443ae48c87a005 | Genome coverage and read depth profiles of plant panel tenfold dilution series samples, from undiluted to 1 in 100,000,000,000 (10−11) across the 6377 nt cucumber green mottle mosaic virus (CGMMV) genome (VPRI43306) detected using Illumina metagenomic sequencing. Each plant panel dilution was tested in triplicate and the technical replicates are shown as different colours. Depth is in standard Logarithmic Scale. | PMC9607580 | plants-11-02716-g004.jpg |
0.489715 | 97b70122df814fffa2238327c1439410 | Genome coverage and read depth profiles of seed panel dilution series samples, from undiluted to 1 in 1000 across the 6377 nt reference cucumber green mottle mosaic virus (CGMMV) genome (NSW3-35) detected using Illumina metagenomic sequencing. Biological replicates are shown in each profile. Each biological replicate was tested in triplicate and the results for each technical replicate are shown as different colours. Depth is in standard logarithmic scale. | PMC9607580 | plants-11-02716-g005.jpg |
0.485665 | 505b2fb336a84d65b5fe69a6b24102ed | Diagram showing alignment of PCR amplicons (black lines) to the cucumber green mottle mosaic reference genome (GenBank Accessions No. NC_001801.1). Coding sequence regions for the 186K protein, 129K protein, movement protein and coat protein are shown. Multiplex primers are shown at predicted binding sites, left (forward) primers are dark green and right (reverse) primers are in light green. (Geneious version 2022.0 created by Biomatters. Available from https://www.geneious.com). | PMC9607580 | plants-11-02716-g006.jpg |
0.463069 | 33f0d44a283a4d479740408250f17da2 | Typical sandwich structure [4]. | PMC9608463 | polymers-14-04267-g001.jpg |
0.440626 | 8658c3d3ea7844698d32fc2c5fcf8c14 | Steps to achieve advanced sandwich structure with extraordinary performance. | PMC9608463 | polymers-14-04267-g002.jpg |
0.471616 | bf295477bee34cb1bdf1fa081b4ac9f6 | Types of cores. | PMC9608463 | polymers-14-04267-g003.jpg |
0.427989 | 4167bc62e5744aebaafb8e58c68527a7 | (a) Lattice sandwich structure [17]; (b) Aluminum foam core design [27]. | PMC9608463 | polymers-14-04267-g004.jpg |
0.530091 | f50573f6318a4b1caaf6c0456b45fa57 | (a) Corrugated core sheet dimension; (b) Assembly procedure [31]; (c) Final sandwich panel; (d) Honeycomb sandwich structure [44]. | PMC9608463 | polymers-14-04267-g005.jpg |
0.518687 | ace1d78331b14c328548be89ebc8c36c | (a) 2D representation of auxetic cellular structure; (b) Representative unit cell [49]; (c) Y-shaped core [52]. | PMC9608463 | polymers-14-04267-g006.jpg |
0.459416 | 9f1a64149d6e4db99349cb0ddde34d63 | (a) Photograph of mold used to fabricate the GFRP core; (b) Profile of the cross-section of a GFRP core; [55] (c) Sketch of a 11 × 11 size circular cell honeycomb; (d) Details of the microsection with relevant dimensions, R: cell radius, t: wall thickness, L: cell length, td: double-wall thickness, Ld: bond length (right) [58]. | PMC9608463 | polymers-14-04267-g007a.jpg |
0.465164 | c84a40e580b44274985cf05ad7f22964 | (a) Sketch of five types of unit cell; Maps of the five sandwich panels with corrugated-core geometric configuration: (b) Cross-sections and (c) Axonometric drawing [62]. | PMC9608463 | polymers-14-04267-g008.jpg |
0.434175 | 359c08364656480eb9baa604647aa32d | (a,b) Illustration of HHTs together with square honeycomb, vertex modified honeycomb, solid walled honeytubes, and polymeric HHT [66]; (c) Regular, first order, and second order hierarchy; (d) Process of converting unit cell from first order to second order hierarchy [70]. | PMC9608463 | polymers-14-04267-g009.jpg |
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