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I have raised the following concerns which is necessary to make this manuscript more scientifically interesting.
| null | null |
What are the p53 status and the apoptosis signaling pathway function in N1S1 cells?
| null | null |
cancers14122959_makarova
| 0 |
This is an interesting scientific study, as it concerns the issue of hepatocellular carcinoma, it also has a clinical aspect.
| null | null |
TTFields frequency scan in rat N1S1 HCC cells.
| null | null |
cancers14122959_makarova
| 0 |
The authors suggested that the therapeutic effects of the combination were apoptosis via autophagy.
| null | null |
Author Response Please see attachment Author Response File: Author Response.docx
| null | null |
cancers14122959_makarova
| 0 |
Response 7: We thank the reviewer for this question.
| null | null |
In the animal study we have now added IHC examination of beclin-1 and of GRP78, a marker for ER stress, as described in results sub-section 3.4.
| null | null |
cancers14122959_makarova
| 0 |
In addition to IHC, I suggest performing western blot using PARP antibody where the full length and cleaved bands are observed in the same blot.
| null | null |
Point 6: Any explanation for why not using cloroquine in vivo to integrate better the in vitro data.
| null | null |
cancers14122959_makarova
| 0 |
I read the manuscript with interest and commend the authors for the work done in the area of liver cancer treatment.
| null | null |
Overall, the authors believe that this research demonstrates potential for concomitant TTFields and sorafenib application in the treatment of HCC.
| null | null |
cancers14122959_makarova
| 0 |
GRP78 levels in the groups treated with TTFields or sorafenib alone remained unchanged from the control, but were elevated 2-fold in the TTFields plus sorafenib group (Figure 4f).
| null | null |
Point 7: Why there is no error bar in the control group of all bar graphs?
| null | null |
cancers14122959_makarova
| 0 |
In this manuscript, the authors, Davidi et al have investigated the effect of TTFields in HCC cells and an animal model, alone or in combination with sorafenib.
| null | null |
*p < 0.05, **p < 0.01, ***p < 0.001, and ****p < 0.0001 relative to time-respective control; two-way ANOVA.
| null | null |
cancers14122959_makarova
| 0 |
HepG2 cells were treated for 6, 24, or 48 hours with 150 kHz TTFields, 3 µM sorafenib, or the two treatments combined, followed by Western blot examination of the autophagy markers beclin-1 and LC3 (d), the ER stress marker GRP78 (e), and the apoptosis marker cleaved PARP (f).
| null | null |
This might be helpful and make the in vitro data more relevant with the data shown in vivo.
| null | null |
cancers14122959_makarova
| 0 |
Response 4: We thank the reviewer for this comment.
| null | null |
Response 7: We thank the reviewer for this question.
| null | null |
cancers14122959_makarova
| 0 |
Point 1: In vitro experiments sub-section in Methods sections lacks many experimental details (e.g., type of plate/flask, plating overnight or not before experiment, number of plated cells).
| null | null |
Concomitant TTFields with Sorafenib Enhances Treatment Efficacy in Vivo: “Tumor histology and immunostaining for beclin-1 and LC3, GRP78, and cleaved PARP were performed to examine autophagy, ER stress, and apoptosis levels, respectively.
| null | null |
cancers14122959_makarova
| 0 |
As shown in the Figure 4 C and D, TTFields were found less effective in terms of reducing the tumors volume and weight when compared with sorafenib.
| null | null |
Were these western blots (Figure 3D-F) repeated 3 times as the material and method mentions?
| null | null |
cancers14122959_perova
| 0 |
Point 7: Why there is no error bar in the control group of all bar graphs?
| null | null |
Point 4: Since Annexin V/7-ADD data was not convincing and the authors observed the minor increase of apoptotic cells after HCC cells were treated with combination of TTFields and sorafenib (when compared to the cells treated with TTFields and sorafenib alone), I suggest to run the WBs to examine the expression of the cleaved forms of PARP and caspase-3 (for both HCC cell lines).
| null | null |
cancers14122959_perova
| 0 |
While I do not know if the authors have the technology to perform TTFields in mice, where for sure they should have done xenograft models with the two human cell lines, why the rat cell line was not studied in vitro using the same experimental strategies as for human cells.
| null | null |
However, I feel that the experiments around this concept were not designed well enough to support their new findings.
| null | null |
cancers14122959_perova
| 0 |
We thank the reviewer for this comment, as these additions add much clarity to the mechanism of action of TTFields in combination with sorafenib and provide a more coherent explanation for the in vivo results.
| null | null |
Point 9: Was Mycoplasma testing done routinely?
| null | null |
cancers14122959_perova
| 0 |
Similarly, low evidence of apoptosis (expression of cleaved PARP) was found in these groups, as shown in Figure 4F.
| null | null |
Overall, the authors believe that this research demonstrates potential for concomitant TTFields and sorafenib application in the treatment of HCC.
| null | null |
cancers14122959_perova
| 0 |
The materials and methods section are elaborated in the details.
| null | null |
However, the exact mechanism of the combination therapy induced cell death is not yet known as the activation of autophagy in the combination therapy was not increased.
| null | null |
cancers14122959_perova
| 0 |
These additions may be seen in Figure 3 and are described in results sub-section 3.4, Autophagy-apoptosis Interplay For Treatment with Concomitant TTFields and Sorafenib: “In order to investigate the mechanism of action of TTFields-sorafenib co-application, HepG2 and Huh-7D12 cells were treated for 6, 24, or 48 hours with TTFields, sorafenib (3µM), or the two modalities together, and then examined for expression levels of various proteins.
| null | null |
As a rule of thumb, when a housekeeping protein gives problems in immunoblotting, there are many other classic protein to switch the investigation (b-actin, vinculin, Ku, etc.).
| null | null |
cancers14122959_perova
| 0 |
However, the limited experimental design and paucity of strong data ask for more experiments to proof the feasibility of this combination for treating HCC.
| null | null |
Is this indeed an anti-tumor effect or a very toxic effect to the liver (including tumor).
| null | null |
cancers14122959_perova
| 0 |
While I do not know if the authors have the technology to perform TTFields in mice, where for sure they should have done xenograft models with the two human cell lines, why the rat cell line was not studied in vitro using the same experimental strategies as for human cells.
| null | null |
Also mention the ‘n’ number in all the experiments involved for invitro and invivo.
| null | null |
cancers14122959_perova
| 0 |
Majority of rodent study in cancer research lose the control group early but the treated groups are followed up for much longer to delineate indeed the efficacy vs. toxicity.
| null | null |
The authors suggested that the therapeutic effects of the combination were apoptosis via autophagy.
| null | null |
cancers14122959_perova
| 0 |
However, I feel that the experiments around this concept were not designed well enough to support their new findings.
| null | null |
The higher changes in expression levels and faster kinetics when TTFields and sorafenib were applied together rather than alone indicate higher stress levels imposed on the cells in the former case.” Figure 3.
| null | null |
cancers14122959_perova
| 0 |
Similar, the graphs illustrating the LC3 expression are not in a proper fit with the images shown in Figure 4D.
| null | null |
Point 9: Was Mycoplasma testing done routinely?
| null | null |
cancers14122959_perova
| 0 |
The in vivo model appears to be performed only time which question the validity of data.
| null | null |
Author Response Please see attachment Author Response File: Author Response.docx
| null | null |
cancers14122959_perova
| 0 |
The apoptosis marker cleaved PARP displayed increased expression in the combined group already after 24 hours, elevating even further after 48 hours of treatment.
| null | null |
Point 3: It will be much better to provide the data to explain the mechanisms illustrating why the monotherapy of TTFields or sorafenib induced autophagy, whereas the tumors treated with combination developed the substantial apoptotic death of tumor cells.
| null | null |
cancers14122959_perova
| 0 |
The rigor science recommends at least 2 independent experiments with at least 7 animals randomized per group.
| null | null |
At least one different dose should have been studied for comparison since this is a completely different tumor environment than the in vitro one.
| null | null |
cancers14122959_perova
| 0 |
We thank the reviewer for this comment, as these additions add much clarity to the mechanism of action of TTFields in combination with sorafenib and provide a more coherent explanation for the in vivo results.
| null | null |
Can this dose observed in vitro on only tumor cells be translated to in vivo work where the tumor microenvironment is totally different?
| null | null |
cancers14122959_perova
| 0 |
The authors indicated that TTField had potential to be a new treatment option of hepatocellular carcinoma.
| null | null |
I have raised the following concerns which is necessary to make this manuscript more scientifically interesting.
| null | null |
cancers14122959_perova
| 0 |
For the monotherapies, cleaved PARP increase was only evident at 48 hours of treatment, and to a lower extent than that in the co-treatment group (Figure 3f).
| null | null |
Response: We thank the reviewer for the positive review.
| null | null |
cancers14122959_perova
| 0 |
Were these western blots (Figure 3D-F) repeated 3 times as the material and method mentions?
| null | null |
Can this dose observed in vitro on only tumor cells be translated to in vivo work where the tumor microenvironment is totally different?
| null | null |
cancers14122959_perova
| 0 |
Were the mice perfused before collecting the tumors?
| null | null |
Point 8: Since sorafenib acts also on angiogenesis, did the authors investigate if TTFields may interfere with anti-angiogenic effect sorafenib-mediated?
| null | null |
cancers14122959_perova
| 0 |
Overall, the authors believe that this research demonstrates potential for concomitant TTFields and sorafenib application in the treatment of HCC.
| null | null |
Point 6: Any explanation for why not using cloroquine in vivo to integrate better the in vitro data.
| null | null |
cancers14122959_perova
| 0 |
The reference protein should have the same intensity since this is the control for equal protein loading.
| null | null |
The rigor science recommends at least 2 independent experiments with at least 7 animals randomized per group.
| null | null |
cancers14122959_perova
| 0 |
This might be achieved by using the combination of so-called Tumor Treating fields (TTFields) with targeted drug, I have the following suggestions about this manuscript: Point 1: The authors demonstrate the efficacy of TTFields in vivo even when used as monotherapy.
| null | null |
Point 1: In vitro experiments sub-section in Methods sections lacks many experimental details (e.g., type of plate/flask, plating overnight or not before experiment, number of plated cells).
| null | null |
cancers14122959_perova
| 0 |
Were the cells from supernatant counted (where are probably the majority of dead cells)?
| null | null |
Response 6: We thank the reviewer for this comment.
| null | null |
cancers14122959_perova
| 0 |
Moreover, there is a discrepancy between the fold changes in tumor weight vs. volume in the combination group vs. untreated group.
| null | null |
I read the manuscript with interest and commend the authors for the work done in the area of liver cancer treatment.
| null | null |
cancers14122959_perova
| 0 |
Point 1: In vitro experiments sub-section in Methods sections lacks many experimental details (e.g., type of plate/flask, plating overnight or not before experiment, number of plated cells).
| null | null |
Other important autophagy and degradation markers like Beclin1 and P62 need to be shown to reflect the regulatory mechanism of TTFields, as well as for the combined treatment with Sorafenib.
| null | null |
cancers14122959_perova
| 0 |
Point 1: In vitro experiments sub-section in Methods sections lacks many experimental details (e.g., type of plate/flask, plating overnight or not before experiment, number of plated cells).
| null | null |
Concomitant TTFields with Sorafenib Enhances Treatment Efficacy in Vivo: “Tumor histology and immunostaining for beclin-1 and LC3, GRP78, and cleaved PARP were performed to examine autophagy, ER stress, and apoptosis levels, respectively.
| null | null |
cancers14122959_perova
| 0 |
Author Response Please see attachment Author Response File: Author Response.docx
| null | null |
Is that timing enough to get protein expression?
| null | null |
cancers14122959_perova
| 0 |
Moreover, many bands belonging to all investigated proteins are truncated, fractured and I identified a lot of troubleshooting in bands due the presence of bubbling when running the blots.
| null | null |
The autophagy marker LC3 also displayed such bi-phasic characteristics, but with a somewhat slower kinetics, showing some elevation at 6 hours of treatment, but higher elevation at the 24 hours time point (Figure 3d).
| null | null |
cancers14122959_perova
| 0 |
The assay results enabled direct measurement of the percentage of the isoforms generated thus facilitating a better understanding of the dominant isoform generated by each variant and their effect on protein coding.
| null | null |
As such, in order to correlate the discussion in this paragraph with the information in table 2, the reader also needs to cross reference Table 1 or 3.
| null | null |
cancers14122960_makarova
| 0 |
I would also stress a little more that FL is almost undetectable.
| null | null |
Lines 342-359 – This paragraph discusses two RAD51C variants, c.404+3A>G and c.705+3A>G, for which mg-FL transcripts were detected in 26.3% and 21.3% or transcripts, respectively.
| null | null |
cancers14122960_makarova
| 0 |
The authors further used the minigene splicing assay results, and understanding of RAD51C protein biology, to evaluate each variant using the most current ACMG-AMP frameworks for variant classification.
| null | null |
Author Response Thank you very much for the positive comments 1-
| null | null |
cancers14122960_makarova
| 0 |
Is it known whether there is a threshold of RAD51C deficiency that is tolerated before associated cancer risks become increased?
| null | null |
The manuscript entitled "Minigene splicing assays identify 20 spliceogenic variants of the breast/ovarian cancer susceptibility gene RAD51C authored by Sanoguera-Miralles et al., is a very interesting study.
| null | null |
cancers14122960_makarova
| 0 |
However, the corresponding tables that summarizes variant classification according to the ACMG/AMP-based criteria (Table 2), does not include these transcript isoform names.
| null | null |
Although a reference is provided so that the reader can look up what these ad-hoc rules are, it would also be helpful to briefly describe these in the current manuscript.
| null | null |
cancers14122960_makarova
| 0 |
Leaking splicing was also noted in two instances resulting in detection of the canonical transcript in 26.3% and 21.3% of transcripts, respectively.
| null | null |
Although a reference is provided so that the reader can look up what these ad-hoc rules are, it would also be helpful to briefly describe these in the current manuscript.
| null | null |
cancers14122960_makarova
| 0 |
Is there evidence from another source that these beta strands are critical to protein function and that their loss is deleterious (rather than resulting in normal or slightly reduced protein activity)?
| null | null |
The transcript analysis results demonstrated splice complexity with several variants resulting in two or more transcript isoforms.
| null | null |
cancers14122960_makarova
| 0 |
While in silico prediction tools can provide insights to possible impacts of nucleotide variants on splicing, these prediction tools do not replace the empirical evidence that the minigene assay can provide.
| null | null |
These variant interpretations were generally evidence based and well thought out.
| null | null |
cancers14122960_makarova
| 0 |
Overall manuscript is well written, very relevant for variant analysis and is suitable for publication if they address the following concerns: 1)
| null | null |
The transcript analysis results demonstrated splice complexity with several variants resulting in two or more transcript isoforms.
| null | null |
cancers14122960_makarova
| 0 |
present an interesting manuscript that utilizes a minigene splicing assay to examine twenty RAD51C variants catalogued through ClinVar.
| null | null |
The manuscript is very well written, clear and well discussed; I have just few comments: in the Introduction in the sentence 61-69 the authors do not mention anything about BRCA1 (they mention MLH1 though); I would expect that as BRCA1 splice variants are deeply studied.
| null | null |
cancers14122960_makarova
| 0 |
The manuscript is very well written, clear and well discussed; I have just few comments: in the Introduction in the sentence 61-69 the authors do not mention anything about BRCA1 (they mention MLH1 though); I would expect that as BRCA1 splice variants are deeply studied.
| null | null |
Lines 342-359 – This paragraph discusses two RAD51C variants, c.404+3A>G and c.705+3A>G, for which mg-FL transcripts were detected in 26.3% and 21.3% or transcripts, respectively.
| null | null |
cancers14122960_makarova
| 0 |
Results section (Page 6, line 221): We investigated the association of NR2F1 expression with metastasis.
| null | null |
Cell proliferation-related gene sets were suppressed and MKi67 expression was lower in high NR2F1 BC.
| null | null |
cancers14122962_makarova
| 0 |
Discussion section (Page 13, line 427): The current study demonstrated that NR2F1 expression in the bulk tumor of primary breast cancer is associated with decreased cell proliferation and cancer stem cell-like characteristics.
| null | null |
In addition, it shows that NR2F1 is primarily expressed in CAFs, particularly in inflammatory CAFs.
| null | null |
cancers14122962_makarova
| 0 |
Conclusions: NR2F1 expression in breast cancer is associated with tumor dormancy traits and it is predominantly ex-pressed in CAFs in the tumor microenvironment.
| null | null |
Nat Cell Biol 2017), it is puzzling how NR2F1 in CAFs of the primary tumor would contribute to dormancy of DTCs.
| null | null |
cancers14122962_makarova
| 0 |
Can the authors do some analyses using data generated from both primary and metastatic tumors to check if there is any difference of NR2F1 expression, and how NR2F1 expression is correlated with metastasis?
| null | null |
Expression of NR2F1 between endocrine therapy responder and non-responder in hormone-positive primary breast cancer.
| null | null |
cancers14122962_makarova
| 0 |
Kaplan-Meier curves of overall survival (OS), disease- specific survival (DSS), and disease-free survival (DFS) based on the high and low NR2F1 expression in triple-negative breast cancer of three large cohorts.
| null | null |
(A) Boxplots showing various scores based on high and low NR2F1 expression in triple-negative breast cancer of TCGA; Intratumoral heterogeneity, homologous recombination deficiency (HRD), silent/non- silent mutation rate, SNV/Indel neoantigen, Interferon gamma response, fraction altered, tumor-infiltrating lymphocytes (TIL) fraction, and stromal fraction.
| null | null |
cancers14122962_makarova
| 0 |
Kaplan-Meier curves of overall survival (OS), disease- specific survival (DSS), and disease-free survival (DFS) based on the high and low NR2F1 expression in triple-negative breast cancer of three large cohorts.
| null | null |
Comment 2: The authors choose chemotherapy as a treatment option to compare it with NR2F1 levels.
| null | null |
cancers14122962_makarova
| 0 |
Previous studies mentioned that NR2F1 was highly expressed in DTCs [67].
| null | null |
Comment 3: Line 170, “Figure 1E” should be “Figure 1D”.
| null | null |
cancers14122962_makarova
| 0 |
In order to prove that CAF-expressed NR2F1 regulates breast tumor dormancy, one needs to analyze the single-cell sequencing data and show whether the late recurrence in other organs, such as lungs, bones, and brain, other than lymph nodes, is correlated with NR2F1 expression in the CAFs.
| null | null |
We found that NR2F1 is most predominantly expressed in CAFs in the TME of primary breast cancer.
| null | null |
cancers14122962_makarova
| 0 |
Comment 2: The authors choose chemotherapy as a treatment option to compare it with NR2F1 levels.
| null | null |
Methods: A total of 6758 transcriptomes of bulk tumors from multiple breast cancer patient cohorts and two single-cell sequence cohorts were analyzed.
| null | null |
cancers14122962_makarova
| 0 |
Results section (Page 11, line 346): There was no difference in NR2F1 expression between responder and non-responder to neoadjuvant endocrine therapy (Supplementary Fig. 9).
| null | null |
Thus, we investigated the clinical relevance of the expression of NR2F1, a known dormancy biomarker.
| null | null |
cancers14122962_perova
| 0 |
However, the findings from our study are not ample to substantiate that CAF-expressed NR2F1 regulates breast tumor dormancy.
| null | null |
Please find our point-by-point responses below.
| null | null |
cancers14122962_perova
| 0 |
Association of NR2F1 with immunity within the tumor microenvironment of HER2 positive breast cancer.
| null | null |
We found that NR2F1 is most predominantly expressed in CAFs in the TME of primary breast cancer.
| null | null |
cancers14122962_perova
| 0 |
Discussion section (Page 13, line 427): The current study demonstrated that NR2F1 expression in the bulk tumor of primary breast cancer is associated with decreased cell proliferation and cancer stem cell-like characteristics.
| null | null |
Man-Whitney U test was used to compare the two groups and p-values are shown in bold for significant results (p < 0.05).
| null | null |
cancers14122962_perova
| 0 |
Also, dot plots show the expression of NR2F1, TGFB1, SOX9, and RARB by cell type in each immunohistological subtype in single-cell Cohort 2.
| null | null |
To this end, we believe that NR2F1 expression in the bulk tumor does not reflect the expression in the cancer cells, thus its value as a biomarker is in doubt.
| null | null |
cancers14122962_perova
| 0 |
Results section (Page 11, line 346): There was no difference in NR2F1 expression between responder and non-responder to neoadjuvant endocrine therapy (Supplementary Fig. 9).
| null | null |
Kaplan-Meier curves of overall survival (OS), disease- specific survival (DSS), and disease-free survival (DFS) based on the high and low NR2F1 expression in triple-negative breast cancer of three large cohorts.
| null | null |
cancers14122962_perova
| 0 |
Log-rank test was used for the analysis, and significant p values are shown in bold.
| null | null |
Nat Cell Biol 2017), it is puzzling how NR2F1 in CAFs of the primary tumor would contribute to dormancy of DTCs.
| null | null |
cancers14122962_perova
| 0 |
Comment 2: The authors should clearly explain the innovation and importance of their work on the introduction of the manuscript.
| null | null |
They should justify the value of the work and compare their work with previously similar published papers.
| null | null |
catal12030290_makarova
| 0 |
According to the reviewer suggestion, the SEM images with same scale are provided in the revised manuscript.
| null | null |
Response: We acknowledge the reviewer’s opinion.
| null | null |
catal12030290_makarova
| 0 |
Comment 5: Why authors did not use XRD technique for sample characterization?
| null | null |
A point-by-point response to the reviewer-2 comments is appended below for your convenience.
| null | null |
catal12030290_makarova
| 0 |
Synthesis of bioadsorbent and evaluation of its structure using SEM and FT-IR spectroscopy.
| null | null |
Comment 2: The authors should clearly explain the innovation and importance of their work on the introduction of the manuscript.
| null | null |
catal12030290_makarova
| 0 |
: catalysts-1584262 Title: Bio-stimulated adsorption of Cr(VI) from aqueous solution by Groundnut Shell Activated Carbon@Al embedded material Response to Reviewer-2 Comments We appreciate the efforts of the reviewers for their detailed and insightful comments, which have helped us to improve the quality of our manuscript.
| null | null |
All the modifications are shown in yellow color in the revised manuscript.
| null | null |
catal12030290_makarova
| 0 |
We hope the reviewer understand the experimental deficiencies at the stage of the present experiments.
| null | null |
There are some points which must be edited or clarified by providing additional information or comments:
| null | null |
catal12030290_makarova
| 0 |
Comments to the Author Manuscript Catalysts-1584262 The manuscript entitled ‘Bio-stimulated adsorption of Cr(VI) from aqueous solution by Groundnut Shell Activated [email protected] embedded material’ by Rao et al focuses on the synthesis of bioadsorbent aluminum metal blended with groundnut shell activated carbon material (Al-GNSC) and it practical application for Cr(VI) adsorption from waste aqueous solutions.
| null | null |
Response: We acknowledge the reviewer’s opinion.
| null | null |
catal12030290_makarova
| 0 |
(ppm) pH Contact Time (min) Adsorbent dosage (g l-1) Maximum adsorption capability (mg g-1) References Activated carbon (AC) prepared from coconut tree sawdust 10 3.0 180 0.2 3.46 [29] Raw coconut fiber 250 1.0 270 10 18.60 [30] Sugarcane bagasse 100 2.0 90 10 1.76 [31] Canadian peat Coconut fiber 50 2.0 4320 25 4.61 4.71 [32] peanut shell (P. Shell), sawdust (S. Dust) and Cassia fistula leaves (C.F.
| null | null |
Comment 2: The porosity of the sample should be measured.
| null | null |
catal12030290_makarova
| 0 |
There are some points which must be edited or clarified by providing additional information or comments:
| null | null |
After making all required minor changes in article it could be recommended for publication.
| null | null |
catal12030290_makarova
| 0 |
Please add the discussion which peak shift is related to Cr, the authors can combine these with the discussion of the mechanism.
| null | null |
The authors are very thankful to the Reviewer for their valuable suggestions for the improvement of the manuscript.
| null | null |
catal12030290_makarova
| 0 |
Response: We acknowledge the reviewer’s opinion.
| null | null |
First of all, authors have to attach a EDX mapping images before/after sorption of chromium ions.
| null | null |
catal12030290_makarova
| 0 |
Response: We acknowledge the reviewer’s opinion.
| null | null |
The authors are very thankful to the Reviewer for their valuable suggestions for the improvement of the manuscript.
| null | null |
catal12030290_makarova
| 0 |
We hope the reviewer understand the experimental deficiencies at the stage of the present experiments.
| null | null |
Page 2 of 2 Comment 5: In the experiment, H2SO4 is added in the first step, what is the purpose?
| null | null |
catal12030290_makarova
| 0 |
Comment 9: The conclusion section should be elaborated and improved.
| null | null |
Response: We acknowledge the reviewer’s opinion.
| null | null |
catal12030290_makarova
| 0 |
Comment 8: In order to confirm proposed mechanism of Cr(VI) adsorption (illustrated on the fig 6) Authors should provide data on adsorption capacity of pristine groundnut shell activated carbon (not modified with Al).
| null | null |
Response: We acknowledge the reviewer’s opinion.
| null | null |
catal12030290_makarova
| 0 |
Examination of prepared adsorbents for Cr(VI) ions removal.
| null | null |
However, we regret that we were not able to investigate the BET analysis due to pandemic situation, which could definitely give us additional information.
| null | null |
catal12030290_makarova
| 0 |
The authors are very thankful to the Reviewer for their valuable suggestions for the improvement of the manuscript.
| null | null |
Is the products suitable for this real condition?
| null | null |
catal12030290_makarova
| 0 |
In such form is rather difficult to make adequate comparison.
| null | null |
Please add the discussion which peak shift is related to Cr, the authors can combine these with the discussion of the mechanism.
| null | null |
catal12030290_makarova
| 0 |
Furthermore, it should be justified why this difference does not influence the comparison of the results with the different cell lines (THP1, Jurkat, Raji).
| null | null |
– the activity of PLY/SLO was prevalent at the initial times of incubation and at relatively high amounts (volume) of supernatants, whereas SLS activity fully developed only after initial lag period but was prevalent when relatively low amounts of supernatants were used in the assays.
| null | null |
cells11010166_perova
| 0 |
Point-by-point response to reviewers: Reviewer 1 comments 1.
| null | null |
Its exact mode-of-action is still not yet fully clarified.
| null | null |
cells11010166_perova
| 0 |
However, given the poor characterization of SLS, its unavailability from commercial providers and its extremely tedious purification, the mechanistic details of the SLS neutralization will require an extensive a project of its own and are beyond the scope of the current study.
| null | null |
This control represents 100% survival and allows us to normalize our survival data.
| null | null |
cells11010166_perova
| 0 |
In one control we challenged the cells with bacterial supernatant without adding liposomes, to determine that the baseline cytotoxicity is in line with the results displayed in the Figure 1.
| null | null |
Figure 2 (for referee inspection only) demonstrates that Alamar blue cell viability assay and Trypan blue live/dead quantification provide results that are identical to those obtained in the cell proliferation protocol used in our study.
| null | null |
cells11010166_perova
| 0 |
The following text was added to the material and method section: The data were normalized to a control incubated with PBS instead of bacterial supernatant (considered as 0% cell death).
| null | null |
We hope that with these changes our manuscript will be considered for publication and we are looking forward to hearing from you.
| null | null |
cells11010166_perova
| 0 |
However, as the immune cell lines have different sensitivities depending on the toxin profile of the tested strain, a similar lethal dose corresponds to different supernatant volumes.
| null | null |
However, authors did not make any attempt to explore this aspect in mechanistic detail.
| null | null |
cells11010166_perova
| 0 |
Figure 2 (for referee inspection only) demonstrates that Alamar blue cell viability assay and Trypan blue live/dead quantification provide results that are identical to those obtained in the cell proliferation protocol used in our study.
| null | null |
It is not clear why they are calling the preparations as the nanotraps.
| null | null |
cells11010166_perova
| 0 |
However, I think that the article can be substantially improved if the following points are addressed: (1)
| null | null |
However, I think that the article can be substantially improved if the following points are addressed: (1)
| null | null |
cosmetics2030248_makarova
| 0 |
All the experiments shown are necessary and sufficient to support the conclusions.
| null | null |
Round 1: and Author Response The article titled “Thermal behavior and free-radical-scavenging activity of phytic acid alone and incorporated in galenic emulsions” is focused on the characterization of phytic acid stability and use in cosmetic formulas.
| null | null |
cosmetics2030248_makarova
| 0 |
The article is well written, and the overall study was well planned and professionally conducted.
| null | null |
Round 1: and Author Response The article titled “Thermal behavior and free-radical-scavenging activity of phytic acid alone and incorporated in galenic emulsions” is focused on the characterization of phytic acid stability and use in cosmetic formulas.
| null | null |
cosmetics2030248_makarova
| 0 |
The article is well written, and the overall study was well planned and professionally conducted.
| null | null |
Round 1: and Author Response The article titled “Thermal behavior and free-radical-scavenging activity of phytic acid alone and incorporated in galenic emulsions” is focused on the characterization of phytic acid stability and use in cosmetic formulas.
| null | null |
cosmetics2030248_makarova
| 0 |
However, I think that the article can be substantially improved if the following points are addressed: (1)
| null | null |
Second Round of Evaluation Round 2: and Author Response
| null | null |
cosmetics2030248_makarova
| 0 |
The article is well written, and the overall study was well planned and professionally conducted.
| null | null |
Second Round of Evaluation Round 2: and Author Response
| null | null |
cosmetics2030248_makarova
| 0 |
Second Round of Evaluation Round 2: and Author Response
| null | null |
The article is well written, and the overall study was well planned and professionally conducted.
| null | null |
cosmetics2030248_makarova
| 0 |
The article is well written, and the overall study was well planned and professionally conducted.
| null | null |
Round 1: and Author Response The article titled “Thermal behavior and free-radical-scavenging activity of phytic acid alone and incorporated in galenic emulsions” is focused on the characterization of phytic acid stability and use in cosmetic formulas.
| null | null |
cosmetics2030248_makarova
| 0 |
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