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in order to provide a wider DUE of novobiocinbrilliant greenglycerollactose nbgl agar dishes of this medium were added to standard media hektoen h salmonellashigella agar ss at all plating steps for stool cultures of human medical routine isolates and samples of veterinary routine isolates nbgl expectedly missed lactoseglycerol positive strains of the serotype senftenberg n hs negative strains n and strains of the typhi serotype n otherwise three strains of serotype virchow were unable to grow on nbgl of positive samples nevertheless overall sensitivities were increased by approximately in the human routine h ss nbgl at the direct plating step h ss nbgl at the enrichment plating step and by in the veterinary one nbgl versus usual media positive predictive values of black centred colonies were significantly higher on nbgl in human routine h ss nbgl at the direct plating step h ss nbgl at the enrichment plating step and in the veterinary one as well nbgl versus usual media these data suggest that nbgl agar does improve salmonella isolation in these kinds of routines and that growth should be made sure before experiments using given strains
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one thousand five hundred ninetyeight patients who underwent cardiac valve replacement were reviewed one hundred fiftytwo patients percent required a second valve replacement the indications for repeat valve replacement were prosthetic valve dysfunction in patients percent development of a new valvular lesion in patients percent simple closure of a perivalvular leak in patients percent NC of the valve poppet in patients percent severe hemolysis or emboli in patients percent and prosthetic valve endocarditis in patients percent the mean preoperative new york health association PET class improved from to in the nonreoperated patients and from to in patients who underwent a second VR similarly the mean cardiac index improved from to and from to in nonreoperated and reoperated patients respectively the OM rate was percent in the nonreoperated patients and percent at second operation in the reoperated patients using actuarial techniques the risk of repeat valve replacement was to percent per year LT survival was compared between groups using actuarial techniques the estimated survival rates at and years were percent percent and percent respectively in nonreoperated patients and percent percent and percent in reoperated patients this T0 has documented the excellent improvement in functional and hemodynamic state T3 second cardiac valve replacement the OM and longterm survival rates were similar to those of the nonreoperated patients patients having repeat cardiac VR can expect good improvement in length and SQ of life
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to facilitate investigation of its PCS and PET properties monoclonal antibodies mabs and a goat polyclonal igg specific for the mouse interferon Th1 receptor were characterized and their potential uses studied eight of the mabs interacted with epitopes on the extracellular domain of the receptor two interacted with epitopes on the intracellular domain and one interacted with an epitope that could not be localized definitively to either region of the mabs the majority were iggs were igms and was an iga relative avidities of the seven that could be determined ranged from to microm both the polyclonal goat igg and mab gr the latter specific for an epitope in the binding site for Th1 blocked binding of the ligand and as expected prevented induction by ifngamma of priming of macrophages for RT cell killing none of the other mabs had an effect despite the fact that gr partially greater than blocked IB of Th1 neither the polyclonal igg nor any of the mabs had an agonist effect the relative usefulness of the antibodies for immunoprecipitation immunoblotting immunoassay and cell staining with and without prior fixation is described the results of ICC staining directly confirmed that the majority of immunologically reactive receptor protein expressed by cells is intracellular to facilitate use by other investigators the hybridomas that produce these mabs will be offered to the american type culture collection
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the management of pregnancies in patients is presented with SLE erythematosus and two patients with primary antiphospholipid syndrome all underwent serial doppler blood flow studies of the umbilical and uterine arteries from weeks of gestation drug therapy was directed at disease activity and the fetus and mother monitored intensively to identify optimum time for delivery end diastolic blood flow edf studies were compared with anticardiolipin antibodies acl and the lupus anticoagulant lac to predict outcome of pregnancy in terms of mortality caesarian delivery hypertension and gestation the absence of enddiastolic blood flow predicted caesarian delivery more accurately the presence of edf and the absence of acl was consistent with a normotensive pregnancy the presence of lac was the best of the three in predicting fetal demise
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spontaneous intracranial hypotension sih is typically manifested by orthostatic headaches that may be associated with one or more of several other symptoms including pain or stiffness of the neck nausea emesis horizontal diplopia dizziness NC in hearing visual blurring or visual field cuts photophobia interscapular pain and occasionally face numbness or weakness or radicular upperlimb symptoms cerebrospinal fluid csf pressures by definition are quite low sih almost invariably results from a spontaneous csf leak only very infrequently is this leak at the skull base cribriform SP in the overwhelming majority of patients the leak is at the level of the spine particularly the thoracic spine and cervicothoracic junction sometimes documented leaks and typical clinical and imaging findings of sih are associated with csf pressures that are consistently within limits of normal magnetic resonance imaging of the head typically shows diffuse pachymeningeal gadolinium enhancement often with imaging evidence of sinking of the BB and less frequently with subdural fluid collections engorged CBF venous sinuses enlarged PIT gland or decreased size of the ventricles RI cisternography typically shows absence of activity over the cerebral convexities even at or hours and early appearance of activity in the kidneys and urinary bladder and may sometimes reveal the level of the leak although various treatment modalities have been implemented epidural blood patch is probably the treatment of choice in patients who have failed an initial trial of conservative management when adequate trials of epidural blood patches fail surgery can offer encouraging results in selected cases in which the site of the leak has been identified some of the spontaneous csf leaks are related to weakness of the meningeal sac likely in connection with a connective tissue abnormality
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plasma free tryptophan is significantly decreased in monopolar depressed patients no evidence was found to suggest that poor nutritional PH prior to hospital admission was responsible for these low levels factors known to influence tryptophanalbumin IB in plasma eg concentration of plasma proteins albumin and nonesterified fatty acids did not account for the low levels of free tryptophan in depressed patients a significant decrease in plasma free tryptophan levels was found in perimenopausal but not in pre or PM female controls this mirrors the decrease in circulating oestrogens although exogenously administered oestrogens do not have any therapeutic tau in relieving mild residual DS of lithium treated patients they increased the levels of plasma free tryptophan clofibrate also displaces tryptophan from plasma protein binding sites in both depressed patients and controls utilization of the increased levels of plasma free tryptophan is reduced in depressed patients a situation therefore exists in depressed patients where the plasma free tryptophan is not only reduced but also leaves the plasma less readily than in control subjects
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the high frequency of thyroid diseases justifies the measurement of SS tsh in regular checkups if found normal in nongoitrous patients this measurement is selfsufficient but pregnant women should also be screened for antithyroid antibodies because they have an increased risk of PP hashimotos disease abnormal tsh concentrations suggesting hyperthyroidism tsh mul or hypothyroidism tsh mul need FT and free t complementary determinations and positive ATA may indicate gravesbasedows disease in case of HT or hashimotos disease in case of hypothyroidism more recently measurement of antitshreceptor antibodies had allowed the diagnosis and a followup tool in some cases of thyroid diseases
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a standing gradient model of the lateral intercellular space is presented which includes a basement membrane of finite solute permeability the solution to the model equations is estimated analytically using the isotonic convection approximation of segel in the case of solute pumps uniformly distributed along the length of the channel the achievement of isotonic transport depends only on the Lp of the cell membranes the ability of the MM to transport water against an adverse osmotic gradient is the sum of two terms the first term is simply that for a wellstirred compartment model and reflects basement membrane solute permeability the second term measures the added strength due to DWI limitation within the interspace it is observed however that the ability for uphill water transport due to DWI limitation is diminished by high cell membrane water permeability for physiologically relevant parameters it appears that the high Lp required for isotonic transport renders the contribution of the standing gradient relatively ineffective in transport against an osmotic gradient finally when the model transports both isotonically and against a gradient it is shown that substantial IEL solute polarization effects are unavoidable thus the measured epithelial water permeability will grossly underestimate the water permeability of the cell membranes the accuracy of the analytic approximation is demonstrated by numerical solution of the complete model equations
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pancreatic segmental autotransplantation in the pig has been considered an attractive MM to study several aspects of pancreas transplantation because of the absence of rejections related to the IS however the frequent presence of anatomical variations in the V1 supply of the left pancreatic segment in the pig makes this MM difficult impairing the access for vascular flushing and revascularization in pancreatic autotransplantation we assessed pancreatic vascular anatomy of landrace pigs group i g n Tx T3 direct reconstruction of the hepatic flow and group ii g n Tx T3 hepaticceliac arterial reconstruction hecar with an iliac vascular VG between the CT and the HA htk histidinetryptophanketoglutarate custodiol and uw UW viaspan solutions were used in total technically successfully transplanted animals htk uw T3 h of cold storage were studied reconstruction time was longer in g than in g p thrombosis of the reconstructed hepatic i.a. occurred more in g than in g vs respectively p pancreatic arterial thrombosis was noticed in animals in g and in in g p ninetyfour percent of pancreas grafts were suitable for cold storage study in g versus for g p no differences were noticed in k values weight of Tx grafts preoperative and h postoperative glycemia for both preservation solutions segmental pancreatic autotransplantation can be successfully performed for cold preservation studies a high percentage of pancreas useable for transplantation can be achieved using hepatoceliac arterial reconstruction htk solution is suitable for flushing and h of preservation for pancreatic grafts in the porcine model
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population based studies of vitamin nutritional status in russia ukranian belarus and baltic states in give evidence about satisfied vitamin a NS and insufficient carotenoids nutrition of population the retinol level below mkdl lower reference level was revealed in of the adults of the children and of the pregnant women the level of carotene below mkdl took place in of the adults of the children and of the Pr women worst vitamin a and carotene nutritional status was detected in adults and children living in mogilevskaya and gomelskaya regions suffering from accident on chernobyl station as well as inhabitants of large industrial centres ekaterinburg orenburg kemerovo norilsk zaporoze with bad ecological situation it was pointed on probable connection between insufficient consumption of carotene and increased risk of cancer and cardiovascular diseases it is recommended the realization of largescale actions upon cardinal improvement of vitamin a and carotene supplying of population
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for many women in kenya their husbands act as gatekeepers to access of healthcare services awareness of the danger signs of OCs is the essential first step in accepting appropriate and timely referral to obstetric care the objectives of this study were to assess mens awareness of the danger signs of obstetric complications and to identify any associated demographic factors
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the records linkage resources of the rochester epidemiology program project were used to identify incident cases of invasive squamous cell carcinoma of the skin in the population of rochester minn between and the overall agestandardized incidence was persons in men in women metastasis occurred in and during an average of years of followup recurrence was seen in while subsequent new lesions occurred in the demographics and course of patients with squamous cell carcinoma were different from either those of bowens disease or basal cell carcinoma in these populationbased data
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the PET role of the cytoskeleton in the control of ion channel activity is unknown in the present study immunocolocalization of na channels with TPS antibodies and fluorescein isothiocyanatephalloidin to stain the cortical cytoskeleton indicates that actin is always present in close proximity to apical na channels in a cells the patchclamp technique was used to assess the effect of cortical actin networks on apical na channels in these a epithelial cells the actin filament disrupter cytochalasin d microgramsml induced hH1 activity in cellattached patches within min of addition cytochalasin d also induced andor increased na channel activity in of excised patches tested within min addition of short AFs greater than microm to excised patches also induced channel activity this effect was enhanced by addition of atp andor cytochalasin d the effect of actin on na channel activity was reversed by addition of the g actinbinding protein dnase i or CR prevented by treatment of the excised patches with this enzyme addition of the actinbinding protein filamin reversibly inhibited both spontaneous and actininduced na channels thus actin filament networks achieved by either depolymerizing endogenous actin filaments by treatment with cytochalasin d the addition of exogenous short actin filaments plus atp or actin plus cytochalasin d regulate apical hH1 activity this conclusion was supported by the observation that the addition of short actin filaments in the form of actingelsolin complexes in molar ratios less than was also ERP in activating na channels we have thus demonstrated a functional role for the cortical actin network in the regulation of epithelial na channels that may complement a structural role for membrane protein targetting and assembly
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we used sequence motifs conserved in sadenosylmethioninedependent methyltransferases to identify putative methyltransferases from the complete genome of the yeast saccharomyces cerevisiae seven sequences with the best matches to the methyltransferase consensus motifs were selected for further study we prepared yeast disruption mutants of each of the genes encoding these sequences and we found that disruption of the yjlc gene is lethal whereas disruptions of ycrc and ydrw lead to slow growth phenotypes normal growth was observed when the ydlw ydrc yhrw and yorw genes were disrupted initial analysis of protein methylation patterns of all mutants by CAA analysis revealed that the ydrc mutant has a defect in the methylation of the deltanitrogen atom of Arg residues we propose that ydrc codes for the methyltransferase responsible for this recently characterized type of protein methylation and we designate the enzyme as rmt protein Arg methyltransferase in addition we show that the methylation of susceptible residues in rmt substrates is likely to take place on nascent polypeptide chains and that these substrates exist in the cell as fully methylated species interestingly rmt has CS identity over amino acids to the rat guanidinoacetate nmethyltransferase an enzyme responsible for methylating the deltanitrogen of the small molecule GAA
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the internet plays an increasingly important role in health education providing laypeople with information about healthrelated topics that range from diseasespecific contexts to GA HP compared to traditional health education the internet allows the use of multimedia applications that offer promise to enhance individuals health knowledge and literacy this T0 aims at testing the effect of multimedia presentation of health information on learning relying on an exp design it investigates how retention of information differs for textonly presentation imageonly presentation and multimedia text and image presentation of online health information two hundred and forty students were randomly assigned to four CG each exposed to a different website version three CG were exposed to the same information using text only image only or text and image presentation a fourth group received unrelated information control group PR was assessed by the means of a recognition test to examine a possible interaction between website version and recognition test half of the students received a recognition test in text form and half of them received a recognition test in imagery form in line with assumptions from dual coding theory students exposed to the multimedia text and image presentation recognized significantly more information than students exposed to the textonly presentation this did not hold for students exposed to the imageonly presentation the impact of presentation style on retention scores was moderated by the way PR was assessed for imageonly presentation but not for textonly or multimedia presentation possible explanations and implications for the design of online HE interventions are discussed
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there is a close relationship between the rates at which DLM and heritable translocations are induced by EMS ems or triethylenemelamine tem in male postmeiotic germ cells this relationship does not hold for isopropyl methanesulfonate ims which induced only negligible frequencies of heritable translocations at doses that induced high C2 of dominant lethal mutations nor does ims behave like ems and tem in the degree to which eggs of different stocks of females repair premutational lesions that are carried in the spermlarge differences between stocks for ims treatment and small differences for ems or tem treatment these dissimilarities between ims and the other two alkylating chemicals are postulated to be attributable to differences in the types of lesions present at the time of repair activity and to whether or not chromosomal aberrations are already fixed prior to postfertilization PN dna synthesis
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data from the national vital statistics system mortality â¢from through the ageadjusted rate for chronic obstructive pulmonary disease copdrelated deaths decreased for men and for women aged and over â¢between and the copdrelated death rate decreased for both men and women aged and for men aged and over the rate increased for both men and women aged and for women aged and over â¢the ageadjusted death rate declined for white men but remained stable for WG women from through â¢the ageadjusted death rate declined for black men but increased for black women from through â¢the major underlying causes of death for copdrelated deaths were copd HR disease and cancer for both men and women
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monitoring progress with disease and injury reduction in many populations will require widespread use of verbal autopsy va multiple methods have been developed for assigning causand to relate it to the number of venipunctures duration of procedure and catheter tip position this was a crosssectional study with prospective data collection conducted in a neonatal intensive ECU of a private hospital in the city of são paulo during the period from august to july which evaluated picc insertions the adoption of analgesic or sedative strategies occurred in catheter placements and was not related to the number of venipunctures duration of procedure or catheter tip position i.v. of midazolam in and fentanyl in catheter insertions were the most frequent strategies wider adoption of analgesic strategies is recommended before during and after the procedure
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the prognostic classification developed by the international germ cell consensus group igccc enables appropriate choice of initial treatment and provides consistent eligibility criteria for clinical trials and more accurate assessment of published results the ST for igccc poor and intermediateprognosis germcell PT is cycles of bleomycin etoposide and cisplatin chemotherapy followed by surgical resection of residual masses if the SS RT markers have returned to normal improved outcomes are achieved by centers that treat a larger number of cases the unsatisfactory results achieved with current therapy warrant entry of these patients into appropriate clinical trials future improvements in therapy are likely to require a better understanding of the molecular mechanisms of resistance and the OD of novel therapeutic approaches that target these mechanisms
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to investigate variations in sex ratio among iranian primary congenital glaucoma pcg patients with and without mutations in the cypb gene and to evaluate possible clinical variations associated with sex in these two CG
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the usefulness of subzonal injection szi for the treatment of severe male factor infertility has been restricted by low and unpredictable fertilization rates and the high risk of polyspermy T3 the injection of multiple spermatozoa in this prospective T0 we have evaluated whether sperm morphology and the percentage of acrosomereacted spermatozoa at the time of injection can be used to predict szi fertilization outcomes populations of motile spermatozoa equivalent to those injected were collected from the mediumoil interface immediately T3 szi of each cohort of oocytes morphology was assessed using the world health organization criteria and the acrosomal status of spermatozoa was determined after IF with rhodamineconjugated pisum sativum agglutinin a fertilization index fi was calculated to express the actual fertilizing potential of the spermatozoa injected in all patients underwent szi cycles the overall fertilization and polyspermy rates were and respectively and a clinical pregnancy rate per transfer of was achieved linear regression analysis demonstrated a statistically significant relationship between morphology and the fi r p patients with or normal morphology always had a fi or and this was reflected by low fertilization and polyspermy rates and the high number of cycles with complete failure of fertilization in this group in patients with normal morphology there were two patterns low or fi or high or fi fertility this was evident in the fertilization and respectively and polyspermy and respectively rates of these two patient subgroupsabstract truncated at words
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sebaceous carcinoma is a rare tumor from the adnexal epithélium of the sebaceous PG usually lesions arise in meibonian PG of the eyelid however extraocular lesions within head and neck have been reported we report a case of recurrent sebaceous carcinoma of the parotid gland without metastases
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foodborne diseases remain an important cause of morbidity in the united states among all age CG a potentially important contributor to this morbidity is improper food handling and preparation practices in kitchens at restaurants and in private homes in the LAC department of public health lacdph established numeric scores for restaurant inspections and posted grades for these inspections publicly by the end of this initiative was credited with helping to reduce by compared with the number of hospitalizations for foodborne infections from nontyphoidal salmonella campylobacter and escherichia coli in the region in the spring of the lacdph environmental health program launched the home kitchen selfinspection program a voluntary selfinspection and education program to promote safer food hygiene practices at home this report describes the implementation of this program and the results from its webbased selfassessment questionnaire the food safety quiz for the initial program period of overall approximately adults completed the quiz received an a rating a b a c and received a numeric score because they scored lower than on the selfassessment use of interactive online learning tools such as the food safety quiz can be used to promote home food safety in the community further research is needed to evaluate and improve the program content and to assess its effect on changing food handling and preparation practices in the home kitchen
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hepatotoxicity of allyl alcohol involves its bioactivation to ACR and subsequent protein sulfhydryl loss and lipid peroxidation however the links between these events and hepatocellular death are not known the purpose of these studies was to examine whether specific signal transduction pathways are associated with allyl alcohol toxicity in hepatocytes inhibition or augmentation of cyclic amp andor protein kinase a pka by rpadonncyclic monophosphorothioate TEA salt or isobutylmethylxanthine had no effect on allyl alcoholinduced cell death h an inhibitor of pka pkc and pkg partially inhibited cell killing by AA whereas chelerythrine chloride a nonselective pkc inhibitor almost completely abolished allyl alcohol cytotoxicity neither nnnhexahydroxynbiphenylndimethanoldimethyl ether a selective pkc alpha and beta inhibitor nor bisindolylmaleimide i an inhibitor of pkc alpha beta and epsilon had any effect on allyl alcohol cytotoxicity in contrast rottlerin a selective pkcdelta inhibitor blocked hepatocellular killing by allyl alcohol cytoprotection by chelerythrine chloride and rottlerin was not the result of inhibition of bioactivation of AA because each inhibitor also prevented cell death from acrolein WB and immunohistochemical techniques revealed that allyl alcohol stimulated phosphorylation and translocation of pkcdelta to hepatocyte CM ie activation and this activity was inhibited by rottlerin cell death appeared to occur via oncotic CN rather than apoptosis based on ssDNA elisa and PI staining together these results indicate that activation of pkcdelta is a critical early event in initiating hepatocyte injury and death from allyl alcohol
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we reviewed etiology and outcome of consecutive neonates admitted to a neonatal unit for investigation of parentreported fever neonates over months tympanic temperature was measured at the emergency department te and core temperature at the neonatal unit tn microbials were isolated in patients te and tn were both °c in of the patients microbial isolation was associated with older median age vs days p empirical antibiotic commencement p and longer hospital stay median vs days p compared with respiratory viral infection patients with bacteremia had high creactive protein p and likely to have comorbidity of meningitis p te â¥Â°c had the highest sensitivity positive likelihood ratio and positive and negative predictive ratios for bacteremia parentreported fever was associated with a incidence of meningitis of bacteremia and of urinary tract infection the majority of neonates with parentreported fever do not have SBI nevertheless recommendations about threshold of antibiotic initiation are difficult and empirical systemic antibiotic coverage must be commenced in those neonates with te â¥Â°c or elevated creactive protein
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the purpose of this T0 was to evaluate histologically in dogs the periodontal healing of walled intraosseous defects in teeth that were subjected to orthodontic movement toward the defects the defects were surgically created bilaterally at the mesial aspects of the maxillary second premolars and distal aspects of the mandibular second premolars of mongrel dogs one week after creating the defects an orthodontic appliance was installed and the teeth were randomly assigned to of treatment groups those in the test group received a titaniummolybdenum alloy rectangular wire spring that performed a controlled tipping root movement and those in the control group received a passive SS wire AS orthodontic movement of the test teeth lasted months and was followed by a stabilization period of another months after which the animals were killed throughout the T0 routine daily plaque control was performed on the dogay cefepime reached its Cmax min T3 the last drug dose when mean values of microgml microgg and microgml were found in SS pancreas and bile respectively in rabbits with ANP and microgml microgg and microgml respectively in controls tissueserum ratios of cefepime were and at and min respectively after the last dose of cefepime in rabbits with acute necrotizing pancreatitis and and respectively at and min in controls it is concluded that the administration of cefepime in rabbits with acute necrotizing pancreatitis resulted in pancreatic tissue levels well above the mics of the common pathogens involved in pancreatic superinfection so that its administration might be proposed for the therapy of superinfection following ANP in humans
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chitosan is not soluble in water which limits its wide application particularly in the medicine and food industry in the present study water soluble chitosan wsc was prepared by hydrolyzing chitosan using hydrogen peroxide under the catalysis of phosphotungstic acid in homogeneous phase AF affecting hydrolysis were investigated and the optimal hydrolysis conditions were determined the wsc structure was characterized by fourier transform infrared spectroscopy the resulting products were composed of chitooligosaccharides of dp the wsc content of the product and the yield were and ww respectively the results indicate that wsc can be effectively prepared by hydrolysis of chitosan using hydrogen peroxide under the catalysis of phosphotungstic acid
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human IP blood monocytes pbmc were isolated by ficollhypaque density gradient and then fractionated by differential adhesion to plastic surface Ad celldepleted pbmc nonreadherent fraction and firmly Ad fraction so obtained from pbmc pbmc themselves and a mixture of the above cells were then sensitized in vitro with sheep erythrocytes srbc so as to produce a primary antigendependent antigenspecific antibody response it appears that Ad celldepleted pbmc produce about twice as many haemolytic Az as compared to total pbmc from to if depleted pbmc are cocultured with firmly Ad or nonreadherent cells the number of haemolytic areas goes down to or up to respectively PET histochemical immunochemical and morphological data suggest that the inhibiting firmly adherent fraction is composed of typical phagocytizing cells while the enhancing cells of the nonreadherent fraction are similar to the dendritic cells described in human blood and some lymphoid organs which do not exhibit AS pinocytic activity but are the principal accessory cells needed to stimulate lymphocyte responses
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during the past few years it has become increasingly evident that residual renal function rrf is an important and independent predictor of poor outcome in patients with chronic kidney disease ckd although the causes of this observation are not fully understood it appears that the loss of rrf impairs both fluid removal and clearance of solutes which in turn leads to uremic toxicity and increased morbidity and mortality there is increasing evidence that patients with ckd develop signs of fluid overload already in the early phases of the disease and this may be a CS- for inflammatory activation recently an inflammatory component was identified in uremic atherosclerotic and nonatherosclerotic cardiovascular disease cvd which have been consistently associated with poor clinical outcome in patients with ckd signs of systemic inflammation occur in parallel to the impairment in renal function and the pathophysiology is most likely multifactorial including a decrease in cytokine clearance T3 glycation endproduct accumulation oxidative stress and principal fluid overload additionally inflammation seems to be a predictor of accelerated loss of renal function in this article we discuss the evidence showing that patients with ckd generally have fluid overload the mechanisms by which impaired renal CF may lead to a chronic inflammatory state and the available information linking fluid overload to accelerated loss of renal function and cvd through inflammation inflammation may lead to the OD of complications of ckd in particular cvd but on the other hand may also lead to a faster progression of renal disease strategies aiming to reduce fluid overload may be useful to reduce cardiovascular morbidity and mortality but also preserve rrf
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insulin resistance has been shown to occur as a consequence of HF however its exact mechanisms in this setting remain unknown we have previously reported that oxidative stress is enhanced in the skeletal muscle from mice with HF T3 myocardial infarction mi this study is aimed to investigate whether insulin resistance in postinfarct heart failure is due to the impairment of insulin signaling in the skeletal muscle caused by oxidative AS mice were divided into four groups sham operated sham sham treated with apocynin an inhibitor of NOX activation mmoll in drinking water mi and mi treated with apocynin T3 wk intraperitoneal insulin tolerance tests were performed and skeletal muscle samples were obtained for insulin signaling measurements mi mice showed left ventricular dilation and dysfunction by echocardiography and increased left VVI enddiastolic pressure and lung weight the decrease in gl level after insulin load significantly att in mi compared with sham insulinstimulated serine phosphorylation of akt and gl transporter translocation were decreased in mi mice by and respectively apocynin ameliorated the increase in oxidative stress and nadph oxidase activities measured by the lucigenin assay in the skeletal muscle after mi it also improved insulin resistance and inhibited the decrease of akt phosphorylation and gl transporter translocation insulin resistance was induced by the direct impairment of insulin signaling in the skeletal muscle from postinfarct HF which was associated with the enhanced oxidative stress via NOX
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sulfasalazine sasp inhibits lipopolysaccharideinduced nuclearfactor kappa b activation and interleukin il production by cultured explants of placenta amnion and choriodecidua bacteriainduced il production in the cervix is a potential mechanism for premature C2 ripening that may lead to PTB our objective was to determine if sasp inhibits il production by endocervical cells stimulated with bacterial pathogens associated with preterm birth
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conventional immunoassays rely on antibodies that provide high affinity specificity and selectivity against a target analyte however the use of antibodies for the detection of smallsized nonimmunogenic targets such as pharmaceuticals and environmental contaminants presents a number of challenges recent advances in protein engineering have led to the emergence of antibody mimetics that offer the high affinity and specificity associated with antibodies but with reduced batchtobatch R2 high stability and in vitro selection to ensure rapid discovery of binders against a wide range of targets in this work we explore the potential of affimers a recent example of antibody mimetics as suitable bioreceptors for the PCD of small organic target EDC here methylene blue target immobilization for affimer characterization was achieved using longchained alkanethiol linkers coupled with oligoethylene glycol lcatoeg using quartz crystal microbalance with dissipation monitoring qcmd we determine the affinity constant k
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reactions of methylhthiazolones with various nalkoxycarbonyl pyridinium salts af led to nalkoxycarbonyl dihydropyridylthiazolones af oxidation of which yielded a new class of pyridylthiazolones af these reactions were applied to the synthesis of other azaarylthiazolones some of these azaarylthiazolones particularly pyridylthiazolones b c and quinolylthiazolones ab showed positive inotropic activity with little chronotropic effect on guinea pig left atria
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infants of IDDM idm are considered as a risk group for atherosclerosis increased aortic intimamedia thickness has been reported in idm the purpose of this study was to assess carotid artery intimamedia thickness caimt left ventricular mass index lvmi and atherosclerotic risk AF in idm
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a double haploid dh population and a recombinant inbred ri population derived from a cross between a japonica male parent azucena and indica female parents ir and ir respectively were used in both field and pot experiments for detecting qtls and epistasis for rice panicle length in different genetic BG and different environments panicle length pl was measured at maturity qtls for pl were detected using single marker analysis and interval mapping epistasis effects on the trait were also analyzed nine qtls were detected in dh population including qtls detected from field experiment and from pot experiment among them qtls mapped on chromosomes and were identified in both field and pot experiments no significant epistasis effect was detected for pl four qtls were detected in ri population among them from field condition and from pot experiment pairs of epistasis loci were detected in ri population one qtl mapped on chromosome and two pairs of epistatic loci were detected in both field and pot experiments one qtl on chromosome four was identified in both populations and one marker locus rg on chromosome one was involved in additive effect in dh population but epistasis effects in ri population
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degenerative changes in the organ of corti were investigated in two genotypes of mice cbaca moderate agerelated lateonset hearing loss and B6 spontaneous earlyonset auditory deterioration ranging in age from to months cbl and from to months cba CS transmission and scanning electron microscopies were used for qualitative and quantitative analyses alterations of deterioration in the cbamouse cochlea showed a lateonset loss of hair cells lipofuscin in the outer hair cells ohcs giant stereocilia on the inner hair cells and elevated pillar heads in cbl mice the primary lesions of the OC include early damage of stereocilia on ohcs missing ohcs and a ruptured reticular lamina giant cilia and elevated pillar heads were not evident in B6 mice in the aged B6 mouse cochlea the whole organ of corti was collapsed and replaced by the supporting cells whilst in the oldest cba mice CR normallooking ohcs still remained even in severely deteriorated Az of the organ of corti the results indicate that the patterns of degeneration of the organ of corti are different between cbaca and B6 mice probably under the control of different genes
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the socalled higher level gait disorders include several types of gait disorders in which there are no L1 modifications in strength tone sensitivity coordination and balance brain activation CS related to walking have been investigated using spect in humans the aim of the study was to investigate BB activation during walking in subjects with highlevel gait disorders due to chronic subcortical vascular encephalopathy
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outpatients with chronic obstructive airway disease received the parasympatholytic bronchodilator ipratropium bromide sch and the sympathomimetic bronchodilator fenoterol from a metered dose inhaler T3 evaluation of the initial values first sch was administered and min later airway resistance was measured then fenoterol was given and a measurement was taken again min later on the following day the aerosol inhalants were given in the reverse CS T3 the first inhalation mg sch proved to be an equally strong bronchodilator as mg fenoterol and airway resistance was decreased to and respectively of the initial value p less than the additional inhalation of the other bronchodilator caused a further slight decrease in airway resistance to a minimum of but was statistically significant p less than only in the case of the fenoterolsch sequence the importance of bronchiolar tonus in regard to fluctuation of airway resistance and the possibility of a specific additive bronchodilatory effect of adrenergics and vagolytics is discussed
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although the clinical range of interventions for coronary arteries is about to mm the range of diameters of peripheral vasculature is significantly larger about mm for human iliac i.a. when the VD is increased the spacing between excitation electrodes on a conductance sizing device must also increase to accommodate the greater range of vessel diameters the increase in the excitation electrodes distance however causes higher parallel conductance or current losses outside of artery lumen we have previously shown that the conductance catheterguidewire excitation electrode distances affects the measurement accuracy for the IP i.a. lumen sizing here we propose a simple solution that varies the detection electrode distances to compensate for parallel conductance losses computational models were constructed to simulate the conductance guidewire with various electrodes spacing combinations over a range of IP i.a. lumen diameters and surrounding tissue electrical conductivities the results demonstrate that the measurement accuracy may be significantly improved by increased PCD spacing specifically an optimally configured detectionexcitation spacing ie or an equidistant electrode interval with a detectiontoexcitation spacing ratio of was shown to accurately predict the lumen diameter ie error over a broad range of peripheral i.a. dimensions mm diameter mm the computational results were substantiated with both exvivo and invivo measurements of IP arteries the present results support the accuracy of the conductance technique for measurement of peripheral REF vessel diameter
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scallops patinopecten yessoensis are extensively cultured and landed in japan during the processing of scallops large amounts of internal organs and shells are discharged as industrial wastes to reduce the burden on the environment ERP utilization and disposal methods of the wastes are required therefore we have screened for useful materials in scallop internal organs and found ultraviolet uv absorbing EDC from scallop ovaries based on uv absorption electrospray ionizationmass spectrometry esims esimsms and nuclear magnetic resonance nmr spectra three uv absorbing compounds were identified as mycosporinelike amino acids maas shinorine porphyra p and mycosporineglycine to investigate whether maas can act as a uv protector for human cells we examined the protective effects of the three maas on human fibroblast cells from uv irradiation all of the three examined maas protected the cells from uvinduced cell death in particular mycosporineglycine had the strongest effect further we found a promotion effect of maas on the proliferation of human skin FB cells from these results it was found that the three maas isolated from scallop ovaries have a protective effect on human cells against uv CS maas have potential applications in cosmetics and toiletries as a uv protectors and activators of cell proliferation
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an electronic surveillance network for monitoring antibiotic resistance in the netherlands has been in operation since seven public health laboratories participate and the system covers about of all bacteriological determinations in the netherlands this paper reports the results of staphylococci isolated in the period about of the staphylococcus aureus isolates in the T0 period were resistant to methicillin this low percentage may be due to the restrictive use of antibiotics and to strict isolation measures aimed at eradicating MR s aureus low frequencies of resistance among methicillinresistant s aureus were found for vancomycin chloramphenicol cotrimoxazole mupirocin lowlevel resistance and fusidic acid twentyone percent of the CoNS were resistant to methicillin low frequencies of resistance among these MR CoNS were those to vancomycin nitrofurantoin doxycycline and amikacin CoNS from cerebrospinal fluid blood and skin were less often resistant to quinolones than isolates from respiratory tract faeces and urine a significant increase in resistance of CoNS to methicillin erythromycin gentamicin and ciprofloxacin was observed in the investigated period but the resistance to doxycycline and cotrimoxazole decreased in the last few years to confirm the determination of methicillin resistance and coagulase production a pcr method was developed which detects both the meca and the coagulase gene the results of the pcr method correlated well with the methicillin mic as determined by an agardilution method
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to determine whether the microdilution technic for antimicrobic susceptibility testing could detect the heteroresistance of MRSA stock cultures MSSA and methicillinresistant were tested against methicillin oxacillin nafcillin and CET each drug was diluted in muellerhinton broth and in MHB with nacl minimum inhibitory concentration mic determinations were made after hours at c and again T3 hours of incubation with one exception methicillin resistance was detected in MHB when the trays were reincubated and read T3 hours earlier detection of methicillin resistance was possible when the penicillinasestable PCs were diluted in BB containing nacl further studies suggest that the addition of nacl to the BB medium might improve the stabilities of methicillin oxacillin and nafcillin during storage of the frozen microdilution trays either nafcillin or oxacillin would appear to be the best representative of the penicillinasestable PCs standardized diskdiffusion tests with cephalothin disks indicated that most MR strains are susceptible to CET however there is reason to believe that such invitro test results might be inappropriate with microdilution tests most methicillinresistant isolates were more resistant to cephalothin than were the methicillinsusceptible strains but the mics for both types of staphylococci were generally within the range of concentrations that can be obtained during therapy the addition of nacl to the microdilution tests with CET did not alter the test results significantly
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experiments n in three conscious goats were performed in a thermoneutral environment to determine the threshold ie the point at which the brain temperature is equal to the carotid blood temperature and Mw ie the difference between brain and carotid blood temperatures as a function of carotid blood temperature of SBC sbc and analyse the thermal inputs affecting them prior to the experiments the animals received carotid loops and an arteriovenous shunt to manipulate head and trunk temperatures independently of each other the mean sbc threshold was degrees c tcarotis and independent of ttrunk when body core temperature was increased from a hypo to a moderately hyperthermic level the sbc threshold was passed before VO2 had reached its minimum and before cutaneous vasodilation occurred the mean sbc Mw was and rose with increasing ttrunk the degree of sbc was principally independent of RHL high levels of heat loss were found without sbc and C1 degrees of sbc were observed at low levels of heat loss the effect of sbc in and around normothermia is to smooth the onset of shivering or panting and to establish a range of internal temperature within which metabolic rate and respiratory heat loss are simultaneously at low levels
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a direct relationship between dialysatetoplasma sodium gradient blood pressure and interdialytic weight gain exists in hemodialysis hd patients the aim of this T0 was to delineate the longterm variability of serum sodium in hd patients
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a singlemode electroactive waveguiding platform capable of measuring spectroelectrochemical responses of surfaceadsorbed redoxactive molecules over a broad spectral BW has been created this new planar waveguide spectrometer is a combination of the previously developed electroactive integrated optical waveguide eaiow dunphy d r mendes s b saavedra s s armstrong n r anal chem with a recently reported simplified approach to broadband coupling bradshaw j t mendes s b saavedra s s anal chem with the use of a commercially available prism as an incoupling element the eaiow can now guide visible light from at least to nm improving upon its previously demonstrated monochromatic nature coupling profiles of various laser lines along with transmission spectra of narrow bandpass filters at various potentials are used to demonstrate the optical characteristics of this broadband eaiow and to compare its response to that of a conventional transmission instrument assessment of spectral resolution performed by measuring the fwhm of various laser lines ranges from to to demonstrate the capabilities of this technology we show the acquisition of absorbance spectra of two different adsorbates cytochrome c and ferrocenedicarboxylic acid as a CF of applied potential subtleties in the redox chemistries of adsorbed molecules which were difficult to monitor with a monochromatic waveguide are readily apparent when using the broadband coupling scheme
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climbing fibers provide one of the two L1 excitatory inputs to the cerebellar SC in an immature animal several CFs form synapses with one PN during postnatal development most climbing fiber innervations with a PN are eliminated and only one strong fiber remains previous studies suggested that this pruning of surplus climbing fiber innervations depends on the neuronal activity we hypothesized that synaptic STDP might play a role in the maturation and refinement of such a climbing fiber projection pattern and examined the STDP properties of synapses between PN days and in mice we found that a hz conditioning stimulation of climbing SF forming relatively strong synapses with a purkinje neuron induced longterm potentiation of the transmission accompanied by a decrease in the pairedpulse ratio of excitatory postsynaptic current amplitudes this was suggestive of an increased probability of presynaptic release however the conditioning stimulation of climbing fibers forming relatively weak synapses induced longterm depression and tended to increase the PP ratio thus the direction of STDP appears to be determined by the strength of synaptic connection longterm depression occurred only in the conditioned climbing fiber whereas longterm potentiation spread to unconditioned climbing fibers a postsynaptic increase in the intracellular ca concentration was required for LT potentiation but not for longterm depression these results reveal the existence of novel presynaptic STDP at immature climbing fiberpurkinje cell synapses which may contribute to the maturation and refinement of the climbing fiber projection DP
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cytochrome p d cypd is a member of the cytochrome p cyp superfamily involved in the biotransformation of drugs and substances of abuse encountered in clinical toxicology among the cyp superfamily the cypd gene is considered as the most polymorphic as more than different alleles have been identified so far cypd genetic polymorphisms have the potential to affect the toxicity of their substrates
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optimal infrainguinal revascularization should provide limb salvage for the longest duration of time it is not known whether limb salvage is longer with an initial belowknee popliteal or tibial in situ saphenous vein graft or with staged bypasses that is an initial aboveknee popliteal prosthetic bypass if feasible followed by a more distal vein graft should the AK prosthetic VG fail a retrospective review of LE V1 reconstructions performed since utilizing polytetrafluoroethylene ptfe umbilical vein or in situ saphenous vein was completed the data were analyzed for differences in limb salvage and prevention of limb threatening ischemia among three subgroups aboveknee prosthetic bypass BK or tibial in situ RSV bypass and staged reconstructions aboveknee prosthetic bypass with subsequent in situ bypass the CG were similar with respect to severity of limb threatening ischemia as indicated by mean preoperative anklebrachial indices cumulative AA limb salvage at months was percent for prosthetic grafts in the AK position percent for in situ saphenous vein grafts in the belowknee or tibial position and percent for staged reconstruction with an initial prosthetic graft to the AK position followed by a distal in situ vein bypass when the prosthetic graft fails
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the influence of feii on the dissimilatory bacterial reduction of an feiii aqueous complex feiiicitrateaq was investigated using shewanella putrefaciens CS cn the sorption of feii on cn followed a langmuir isotherm leastsquares fitting gave a maximum sorption capacity of qmax x mol cells mmolm of cell SA and an affinity coefficient of log k the growth yield of cn with respect to feiiiaq reduction showed a linear trend with an average value of x cellsmmol of feiii the reduction of feiiiaq by cn was described by monod kinetics with respect to the QA concentration feiiiaq with a halfsaturation constant ks of mm and maximum growth rate micromax of h however the pretreatment of cn with feiiaq significantly inhibited the reduction of feiiiaq resulting in a lag phase of about h depending on initial cell concentrations lower initial cell concentration led to longer lag phase duration and higher cell concentration led to a shorter one transmission electron microscopy and SE dispersive spectroscopy revealed that many cells carried surface precipitates of fe mineral phases valence unspecified during the lag phase these precipitates disappeared T3 the cells recovered from the lag phase the cell inhibition and REC mechanisms from feiiinduced mineral precipitation were not identified by this study but several alternatives were discussed a modified monod MM incorporating a lag phase feii adsorption and AQ complexation reactions was able to describe the experimental results of microbial feiiiaq reduction and cell growth when cells were pretreated with feiiaq
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major predictors of alzheimers disease ad include apolipoprotein e apoeepsilon hippocampal atrophy on magnetic resonance imaging mri and memory dysfunction prior to diagnosis we examined normal elderly subjects with mri and the california verbal learning test cvlt returned for longitudinal followup years later analyses at baseline revealed significant variance in HV accounted for by cerebral volume and age but not by apoe isoform however interactions involving apoe isoform and laterality were observed as hypothesized an apoe x time interaction was revealed for cvlt longdelay free recall apoeepsilon subjects had significantly poorer performance than apoeepsilon subjects at followup forward stepwise multiple SRA predicting followup longdelay free recall selected baseline recall followed by number of apoeepsilon alleles followed by lefthippocampal volume age and sex did not enter into the model we conclude that apoeepsilon predicts longitudinal SM decline in HCs and that mri morphometry of hippocampus adds slightly to predictive value
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in order to evaluate the short and midterm results of percutaneous aortic balloon valvuloplasty consecutive elderly patients with symptomatic severe calcific aortic stenosis underwent the procedure consecutively with followup by clinical DUE and doppler echocardiography over a mean followup period of months there were deaths and patients underwent subsequent AV replacement doppler echocardiography revealed an increase in aortic valve area from cm to cm T3 the procedure but there was a significant trend toward restenosis by months followup in of patients restenosis was accompanied by symptomatic deterioration in of patients although PBV may often improve haemodynamics and relieve symptoms these benefits seem to be shortlived in most cases restenosis has a high rate of occurrence aortic PBV should be reserved for truly inoperable cases and for haemodynamicallyunstable patients who may later undergo surgery
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a decrease in LG sec function is closely related to aging and leads to an increased prevalence of dry eye syndrome since CR cr is considered to prevent functional decline of various organs due to aging we hypothesized that cr could prevent agerelated lacrimal dysfunction sixmonthold F344 rats were randomly divided into ad libitum al and cr CG after months of cr tear CF was examined under conscious state after euthanasia lacrimal glands were subjected to histological examination tear protein secretion stimulation test with carbachol and assessment of oxidative AS with OH deoxyguanosine ohdg and hydroxynonenal hne antibodies cr significantly improved tear volume and tended to increase tear protein secretion volume after stimulation with carbachol compared to al the acinar unit density was significantly higher in the cr rats compared to al rats lacrimal PG in the cr rats showed a lesser degree of interstitial fibrosis cr reduced the concentration of ohdg and the extent of staining with hne in the lacrimal gland compared to al furthermore our electron microscopic observations showed that mitochondrial structure of the LG obtained from the MA cr rats was preserved in comparison to the al rats collectively these results demonstrate for the first time that cr may attenuate oxidative stress related damage in the LG with preservation of LG functions although molecular mechanisms by which cr maintains LG function remains to be resolved cr might provide a novel therapeutic strategy for treating dry eye syndrome
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free sulfhydryl groups in sulfur EDC have been reported to act directly on NK toxins to reduce toxicity the objective of this study was to reduce PIs and glycoalkaloids in simulated snack foods by the addition of sulfurcontaining compounds prior to extrusion B1 methionine and benzyl disulfide were added to potato flakes at levels of or prior to twinscrew extrusion total and free thiols and protease inhibitors were monitored before and after extrusion by colorimetric assays potato glycoalkaloids were analyzed by hplc and by immunoassay extrusion reduced potato flake disulfide bonds disulfide bonds were higher in samples containing added sulfur compounds trypsin inhibitor activity was reduced by as much as by extrusion plus methionine extrusion significantly reduced CPI but only when benzyl disulfide and Met were not added one percent Met and thiamine resulted in reductions in glycoalkaloids
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the transplantation of CP human amniotic membrane has been introduced recently for the reconstruction of the ocular surface however in some diseases the SPK usually dissolves rather quickly and early detachment may occur therefore we tried to stabilize the amniotic transplant by applying glutaraldehyde for collagen crosslinking of the membrane
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we have used MD combined with sagittal transection of the optic chiasm mdox in an attempt to separate the contributions of competitive binocular interactions from those of deprivation per se cell body measurements were made in the intact a laminae of cats reared with mdox these measurements were compared to matched measurements in normallyreared cats cells in the deprived a lamina of the mdox cats were smaller than normal to a degree consistent with other reports involving bd and mde also consistent with the absence of competitive imbalances cells in the nondeprived a lamina of these cats were normal in size exhibiting no sign of hypertrophy
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we analyzed the growth DP of RT masses and the survival of asymptomatic italian patients with a total of small less than or equal to cm in diameter hepatocellular carcinomas arising from cirrhosis the TL of the observation period ranged from to days with an average of mean sd Td ranged from to days mean sd median days three different growth patterns were recognized a tumors with no or very slow initial growth DP doubling time greater than days cases b tumors with declining growth rate over time cases and c PT with almost constant growth rate cases using the SDA we found a score based on albumin alcohol intake number of nodules echo DP and histological type that allowed a correct prediction of short doubling time less than or equal to days in medium doubling time to days in and long doubling time greater than days in of cases the estimated survival rate of the patients calculated by the KM method was at yr at yr and at yr stepwise discriminant analysis showed that a score based on sex hbsag status alcohol consumption ascites gammaglutamyltranspeptidase prothrombin time C-P class and all the sonographical parameters could predict yr survival in of cases we conclude that great variability of growth patterns exists among and within small hepatocellular carcinomas prediction of subsequent growth rate is unreliable in most casesabstract truncated at words
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the aim of this study is to explain the origin of the anomalies and variations that affect the MTC of the stapes these are interpreted from the MM of the stapes muscle differentiation formed by two independent anlagen rodrÃguezvázquez and rodrÃguezvázquez et al one for the belly muscle located on the second branchial arch medially to the facial nerve and another for the tendon formed from the internal segment of interhiale the GPe of the interhiale usually disappears the pyramidal eminence is organized from the mesenchymal condensation sorrounding the muscle belly which is independent of the second branchial arch cartilage
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an immunochromatographicbased CA quixtrade mark e coli o sprout assay and a polymerase chain reaction pcrbased CA taqman e coli oh kit were used to detect escherichia EHEC CS in spent irrigation water from alfalfa sprouts grown from artificially contaminated seeds ten or seeds contaminated by immersion for min in a suspension of e coli oh at concentrations of or cfuml were mixed with g of noninoculated seeds in plastic trays for sprouting the seeds were sprayed with tap water for s every hour and spent irrigation water was collected at intervals and tested e coli oh was detected in nonenriched water by both the taqman pcr of samples and the immunoassay of samples in water collected h from the start of the sprouting process however enrichment of the spent irrigation water in BB HR infusion bhi BB at degrees c for h permitted PCD of e coli oh in water collected h from the start of sprouting using both methods even in trays containing as few as inoculated seeds the taqman pcr CA was more sensitive more positive samples were observed earlier in the sprouting process than the immunoassay however the immunoassay was easier to perform and was more rapid at h T3 the start of the sprouting process the sprouts were heated at degrees c for s to determine the effectiveness of blanching for inactivation of e coli oh all of the samples tested with the taqman assay and of samples tested with the quixtrade mark assay gave positive results for e EHEC T3 enrichment of the blanched sprouts at degrees c for h in addition the organism was detected on rainbow agar o in of samples after h of enrichment of the blanched sprouts in conclusion e coli oh was detected in spent irrigation water collected from sprouts grown from artificially contaminated seeds by both the taqman and quixtrade mark assays the data also revealed that blanching may not be effective to CR inactivate all the e EHEC that may be present in sprouts
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the concentrations of ENR were measured in the tears saliva and serum of cats with signs of upper respiratory tract infection and eight with no signs T3 daily doses of mgkg enrofloxacin concentrations above the minimum inhibitory concentration of chlamydophila felis were found in the saliva and tears of the cats with and without signs of upper RT infection in a prospective randomised CT the efficacy of enrofloxacin against c felis infection in cats with conjunctivitis was compared with the efficacy of doxycycline twentyfive cats were randomly assigned to treatment with either ENR or doxycycline for days of the cats tested positive for c felis by an immunofluorescent antibody test on conjunctival swabs the two treatment groups showed equal improvements in the clinical signs of conjunctivitis and c felis infection status in each group three cats were still c felis antigenpositive after the day course of treatment indicating a persistent infection no SE were observed in the cats treated with ENR
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highly purified radiolabeled mouse hepatitis virus mhv a contained a previously overlooked protein which coelectrophoreses with the gene b product immunoprecipitated from INF cells the gene b protein is posttranslationally acylated rb antibody raised against a recombinant gene b protein expressed in escherichia coli neutralized viral infectivity in the presence of complement although not in the absence of complement immunofluorescent staining of mhvinfected cells with two antipeptide antibodies revealed that the gene b product is membraneassociated and is transported to the cell surface findings consistent with the prediction of a membranespanning segment in the gene b polypeptide these results suggest strongly that the gene b polypeptide represents a new mhv virion env protein which is rat to the tgev orf and ibv c proteins
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ten year survival rate following modified radical mastectomy for breast CA with stage i was per cent sixteen per cent of them had metastases in the axillary CL nodes however setting limits to under centimeter in diameter of the RT rate of the axillat mmhg of MAP blood pressure constantly a decrease in lvedp from to mmhg reduced stroke work index in normal heart but increased slightly in failing heart further lowering in lvedp resulted decreasing in SW index in both group thus modest and adequate reduction in preload and afterload has beneficial effects to cardiac function in contrast further decrease will worsen myocardial perfusion and cardiac CF
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brainderived neurotrophicfactor bdnf is expressed in the retina and controls the OD of subtypes of amacrine cells in the present study we investigated the effects of bdnf on amacrine cells expressing vasoactive intestinal polypeptide vip rats received three intraocular i.v. of bdnf on PN days p and the animals were sacrificed on p p p p and p and vip expression in their retinas was detected by immunohistochemistry p p and by radioimmunoassay ria p p p p p to assess the time course of bdnf effects on vip a significant increase in the density of vippositive amacrine cells was detected in bdnftreated retinas and vip concentration was upregulated by both at p and at p with respect to UT controls vip concentration then slowly declined in the treated retinas over a period of months however a statistically significant increase of was still detectable on p the impact of endogenous bdnf on the regulation of vip expression in the retina was analyzed in mice homozygous for a targeted deletion of the bdnf gene locus bdnf vip immunohistochemistry revealed a marked reduction of vippositive amacrine cells and of vipimmunopositive processes in the inner plexiform layer of the bdnf KO mice lacking bdnf expressed only of the vip protein in their retinas compared with the retinas of wildtype mice as measured by ria our data show that bdnf is a major regulator of vip expression in retinal amacrine cells and exerts longlasting effects on vip content
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multifocal motor neuropathy mmn is a newly recognized disorder with a characteristic clinical picture the diagnosis of mmn is established by the presence of a TPS electrodiagnostic abnormality conduction block confined to motor axons and in some instances by the associated high titers of antigm antibodies clinical improvement may be achieved by treatment with intravenous immunoglobulin or cyclophosphamide which underlines the importance of recognizing these patients we present two patients with slowly progressive predominantly distal weakness and muscle wasting of an upper extremity characteristic of mmn extensive electrophysiological examinations are necessary to demonstrate conduction block of motor axons which distinguishes patients with mmn from patients with motor neuron disease other diagnostic possibilities are discussed
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the biological MICs of several ovine chorionic somatomammotropin ocs derivatives obtained by chemical modification of the lysine residues were studied by radioreceptor assays using rb mammary homogenates lactogenic activity la and CL homogenates somatotropic activity sa even if the control treatment with bh markedly decreased the la it was clear that methylation mainly affected the sa and that ethylation reduced both MICs guanidination inactivated almost completely both activities and acetimidination at a very low degree of lysines led to less than of both MICs these results show the involvement of lysine residues in the interaction of ocs with lactogenic and somatotropic receptors
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smokingcessation rates may be improved by nicotinereplacement therapy that reduces withdrawal symptoms however NIC may have adverse effects on coronary circulation the purpose of this T0 was to examine the shortterm effects of NIC gum chewing on the dimensions of coronary arteries of patients with PD disease and on the response of coronary vessels to sympathetic stimulation caused by the CP test in patients who were past chronic cigarette sm crosssectional areas of PD stenoses and adjacent apparently normal segments were measured by using quantitative coronary angiography at baseline and after a cold pressor test before and T3 NIC gum chewing the cold pressor test produces an increase in arterial pressure without any NC inions ranging from and m resulted in a significant reduction in the secreted elafin protein as measured in the cell supernatant these results provide further evidence for a role of elafin in the regulation of hne driven proteolysis of the EM
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recently magnetic resonance properties of cerebral GM have been spatially mappedin vivoover the cortical surface in one of the first neuroscientific applications of this RPA this study explores what can be learned about auditory cortex in living humans by mapping longitudinal EC50 rate r a property related to myelin content gray matter r and thickness showed repeatable trends including the following regions of high r were always found overlapping posteromedial heschls gyrus they also sometimes occurred in planum temporale and never in other parts of the superior temporal lobe we hypothesize that the high r overlapping heschls gyrus which likely indicates dense gray matter myelination reflects AEP koniocortex ie primary cortex a heavily myelinated area that shows comparable overlap with the gyrus high r overlapping heschls gyrus was identified in every instance suggesting that r may ultimately provide a marker for koniocortex in individuals such a marker would be significant for AEP neuroimaging which has no standard means anatomic or physiologic for localizing cortical Az in individual subjects interhemispheric comparisons revealed greater r on the left on HG PT STG and STS this asymmetry suggests greater gray matter myelination in left auditory cortex which may be a ATP for the left hemispheres specialized processing of speech language and rapid acoustic changes these results indicate that in vivo r mapping can provide new insights into the structure of human CGM and its relation to CF
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the newly developed laminin modified infectionpreventing collagen membrane consists of a component laminate comprising outer collagen layers and a central laminin layer the outer collagen layers dense and porous layers were fabricated by airdrying and freeze drying respectively and the laminin layer was formed by a straightforward liquid coating method in addition hyaluronan based MPs containing silver sulfadiazine agsd were incorporated into the collagen layers agsd content microgcm laminin coated collagen surfaces did not promote FB attachment but showed a retarded FB proliferation rate and an increased rate of collagen synthesis versus pure collagen surfaces in an animal T0 a laminin coating on a nonmedicated collagen membrane significantly increased both wound size reduction and vessel proliferation days after application versus polyurethane film interestingly the laminin coated agsd medicated collagen membrane demonstrated higher wound size reduction and vessel proliferation and lower inflammation than the polyurethane control suggesting that the laminin agsd medicated collagen membrane substantially improves dermal wound healing
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arylsulfatase a asa degrades sulfatide seminolipid and lactosylceramide sulfate glycolipids recognized by the sulph i antibody although sulfatide is considered the main antigen sulfatide is myelin associated but studies have shown a minor distribution also in nonmyelin forming cells the aim of this work was to further T0 sulfatide in neurons and astrocytes by immunohistochemistry facilitated by investigation of tissue from adult asa deficient asa mice cells with a low presence of sulfatide might be detected due to lack of asa activity and accumulation of sulph i antigens sulfatide positive astrocytes and neurons were more numerous and intensely stained in asa mice demonstrating a sulfatide accumulation compared to controls sulph i IF was especially increased in the molecular layer of cerebellum in which purkinje cell dendrites displayed an altered morphology and in SL ivvi of cerebral SC in hippocampus immunostaining was found in neuronal cytoplasm in asa but in nuclear membranes of control mice we observed a gray matter astrogliosis which appeared to be associated to sulfatide accumulation in addition the developmental NC months of sulph i antigens galactosylceramide phospholipids and cholesterol were followed by lipid analyses which verified sulfatide and seminolipid accumulation in AD asa mice although no lactosylceramide sulfate could be detected in addition to demonstrating sulfatide in neurons and astrocytes this study supports the value of asa mice as a model for metachromatic leukodystrophy and suggests that accumulation of sulfatide beyond myelin might contribute to the pathology of this disease
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to attempt description of the set of mrna and protein sialome expressed in the SGs of the tick ixodes scapularis we randomly sequenced clones of a fulllength SSG cdna library of this arthropod and performed edman Kd of protein bands from salivary gland homogenates sgh and saliva separated by sdspage the sequences were grouped into clusters of which are not associated with known i scapularis sequences and protein bands from page yielded aminoterminal information on the saliva and salivary gland gels respectively we attributed of these sequences to translation products of the cdna library FL sequences were obtained for clones among these protein sequences are several protease inhibitors of distinct classes metalloproteases novel proteins with histaminebinding domains and several peptide families of unknown CF displaying different conserved cysteine residues many of which contain single kunitz domains this work provides information into the diversity of messages expressed in the salivary glands of i scapularis describes novel sequences that may be responsible for known biological activtamen in mdma treated when compared to control rats in the social SM test mdma significantly impaired rats ST working memory these results show that chronic SE to mdma strongly affects preprotachykinin a mrna levels in discrete rat brain regions these changes occur in experimental conditions in which working SM is markedly reduced suggesting that changes in gene expression of tachykinin mechanisms may contribute to the effects of mdma on memory CF
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to compare the enamel whitening effect of a minute dielectrophoresis enhanced electrochemical delivery to a minute DWI treatment
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a fulllength cdna sequence putatively encoding an atpbinding cassette abc transporter gintabc was isolated from the extraradical mycelia of the arbuscular mycorrhizal fungus glomus intraradices bioinformatic analysis of the CS indicated that gintabc encodes a amino acid polypeptide containing two sixtransmembrane clusters tmd IC with sequences characteristics of the nucleotide binding domains nbd and an extra nterminus extension tmd gintabc presents a predicted tmdtmdnbd topology typical of the multidrug resistanceassociated protein subfamily of abc transporters mRNA analyses revealed no difference in the expression C2 of gintabc in the extra vs the intraradical mycelia gintabc was upregulated by cd and cu but not by zn suggesting that this transporter might be involved in cu and cd detoxification paraquat an oxidative agent also induced the transcription of gintabc these data suggest that redox changes may be involved in the transcriptional regulation of gintabc by cd and cu
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we report an instructive case of a yearold man who presented with a dumbbell shaped TB PA across the greater sciatic notch bilaterally compressing both sciatic SN clinical symptoms PD slowly and mimicked lumbar radiculopathy thus delaying an accurate diagnosis AL retroperitoneal and posterolateral gluteal approaches of the greater sciatic notch as well as the acetabulum on both sides were followed in order to provide safe viewing and resection of the abscess the PA wall was adherent to the sciatic nerve and surrounding blood vessels the PS completely disappeared T3 resection of the PA
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a sialicacidbinding lectin with specificity for nglycolylneuraminic acid neugc was purified from the hemolymph of the marine crab scylla serrata by affinity chromatography using thyroglobulincoupled agarose the IB specificity of scylla lectin distinguishes it from other known sialicacidspecific lectins found in LPA and LFA which show a broader range of specificity for sialic acids the molecular mass of the purified lectin is about kda under reducing conditions sdspage it resolved into two subunits of kda and kda neugc inhibited HA activity of the purified lectin at a concentration as low as mm whereas nacetylneuraminic acid neuac even at a concentration of mm failed to inhibit hemagglutination this finding was supported by potent inhibition of HA by bovine and porcine thyroglobulins which contain a neugc alpha gal as terminal component of oligosaccharide residues neither glycoproteins glycophorin nn PSM which contain neuac alpha galgalnac and neuac alpha galnac nor human acid glycoprotein which contains neuac alpha alpha gal or colominic acid a sialopolymer with neuac alpha neuac inhibited the lectin activity the specificity of the lectin for neugc appears to account for the fact that it agglutinates rabbit and mice erythrocytes but not human a o ab rat or chicken erythrocytes which contain neuac the inability of the lectin to agglutinate erythrocytes horse that prominently express neugc could be due to oacetylation of neugc in support of this bovine submaxillary mucin which contains oacetylated neugc inhibited the HA of the lectin better T3 removal of oacetyl CG by base treatment the unique specificity of scylla lectin is of diagnostic potential for human cancer tissues expressing neugc since neugc is not found in normal human tissues
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mechanical stresses due to blood flow regulate V1 endothelial cell structure and function and play a key role in arterial physiology and pathology in particular the development of atherosclerosis has been shown to correlate with regions of disturbed blood flow where endothelial cells are round and have a randomly organized cytoskeleton thus deciphering the relation between the mechanical environment cell structure and cell function is a key step toward understanding the early OD of atherosclerosis recent experiments have demonstrated very rapid ⼠ms and longdistance ⼠μm cellular mechanotransduction in which prestressed actin stress fibers play a critical role here we develop a model of mechanical signal transmission within a cell by describing strains in a network of prestressed viscoelastic AS fibers following the application of a force to the cell surface we find force transmission dynamics that are consistent with exp results we also show that the extent of stress fiber alignment and the direction of the applied force relative to this alignment are key determinants of the efficiency of mechanical signal transmission these results are consistent with the link observed experimentally between cytoskeletal organization mechanical AS and cellular responsiveness to stress based on these results we suggest that mechanical CS of actin SFs under force constitutes a key link in the mechanotransduction chain
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use of internal mammary vessels during breast reconstruction provides information on part of the internal mammary chain lymph nodes lns it was evaluated whether our current practice of screening should be changed to identify those delayed breast reconstruction patients with tumorpositive internal mammary NO imns and whether breast reconstruction should be continued in case suspicious imns were found intraoperatively from february to december patients had received deep inferior epigastric perforator flaps for delayed breast reconstruction prospectively collected data for suspicious internal mammary lns were evaluated in patients who had received a delayed breast reconstruction macroscopically suspicious lns were detected in the course of the IM chain three patients had a pathologic diagnosis of malignancy which was found to match their primary RT no relationship between positive internal mammary chain lns and location of the primary tumor tnmstage or previously po adjuvant therapy was found suspicious internal mammary chain lns found during recipient vessel dissection for breast reconstruction can have important consequences for treatment of malignant disease in individual patients presented data do not support changing the current perioperative approach of delayed breast reconstruction
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midair collisions cause loss of life and property and undermine general aviation they also represent failures of the seeandavoid concept in air traffic control this study identifies limitations of the seeandavoid concept
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notch signaling is an evolutionarily conserved mechanism used by invertebrates and vertebrates to control cell fates through closerange cell interactions four notch receptors have been identified in vertebrates and different ligands divided into deltalike and serratelike jagged several studies have demonstrated that notch signaling is involved in different branches of the cell fate decision tree differentiation proliferation and apoptms at ambient temperature are highly susceptible to microbial contamination and formation of biofilm the impact of two disinfection regimens with ozone as a function of time the heterotrophic plate counts hpc and the concentration of total SOCs toc in purified water were investigated over a period of years we have established that concentrations of ozone of ppb in the production regimen and ppb in the disinfection regimen are sufficient to maintain a low bioburden and low toc in a recirculating distribution system the purified water that entered into the distribution system has low hpc cfuml indicating a reduction by ozone in the storage tank by up to approximately fold over years of the microbial counts were in the category cfuml and none in category or cfuml in spite of increased toc in the inlet water up to ppb the microbial counts in purified water in the distribution loop were unaffected the study emphasizes that the critical points regarding microbial contamination of the purified water system are user point valves and the tubes used for transferring water to equipment the specified ozone level prevents microbial growth and formation of biofilm in the CSD system that might otherwise endanger the water SQ by sporadic PR of microbes
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in this work a series of polylactic acidsio nanocomposites have been prepared by a melt mixing procedure the dispersion quality was examined by SEM microscopy to study the Kd behavior of the polylactic acidnanocomposites prepared the samples were immersed in a buffer solution at a temperature of â with a ph of for a time period of up to weeks these conditions simulate those in the human body AGA in medical applications in order to assess their suitability in biomedical applications we investigated the biocompatibility of these materials in terms of cell viability growth and morphology a good initial cell adhesion has been detected supporting their potential use in bone tissue engineering applications the hydrolytic Kd of PLA under the prescribed conditions was studied by the molecular weight reduction in terms of size exclusion chromatography whereas the progress of thermal stability of PLA and polylactic acidnanocomposites during aging was tested by TG analysis the evolution of the materials thermomechanical properties during aging was studied by differential scanning calorimetry dynamic mechanical analysis and tensile testing the crystallization SMB in PLA and the way it is affected by the presence of nanofillers during degradation procedure has been studied and values of crystallinity increment have been found at the specific aging conditions studied Si nanoparticles accelerate the degradability of polylactic acid having a higher impact on youngs modulus under the specified aging conditions for weeks and hereafter this acceleration is retarded due to the crystallinity increment as a result of the molecular weight reduction
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we collected data on patients from families with guanidinoacetate methyltransferase gamt deficiency global developmental delayintellectual disability ddid with speechlanguage delay and behavioral problems as the most affected domains was present in participants with additional epilepsy present in and movement disorder in treatment regimens included various combinationsdosages of creatinemonohydrate Orn sodium benzoate and proteinarginine restricted diets the median age at treatment initiation was and months in patients with mild and moderate ddid respectively and years in patients with severe ddid increase of CBF creatine and decrease of plasmacsf guanidinoacetate levels were achieved by supplementation with creatinemonohydrate combined with high dosages of lornithine andor an argininerestricted diet mgkgd larginine therapy was associated with improvement or stabilization of symptoms in all of the symptomatic cases the patients treated younger than months had normal or almost normal developmental outcomes one with inconsistent compliance had a borderline iq at age years an observational gamt database will be essential to identify the best treatment to reduce plasma guanidinoacetate C2 and improve LT outcomes
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the D2 amygdala is a group of structures including the CE and medial amygdaloid nuclei bed nucleus of the ST and sublenticular substantia innominata this group of structures is thought to be important in a variety of psychiatric disorders many of which are linked in one way or another to monoamines and their transporters however not much is known about the CSD of these molecules in the primate D2 amygdala thus we mapped the CSD of SF immunoreactive for IMT hydroxylase dopamine betahydroxylase serotonin dopamine transporter and serotonin transporter in the brains of macaque monkeys tyrosine hydroxylase serotonin and serotonin transporterimmunoreactive fibers were found in highest concentrations in the lateral division of the central nucleus and lateral dorsal part of the bed nucleus of the ST dopamine betahydroxylaseimmunoreactive SF were found ieeper cavities were covered with calcium XTT andor glass ionomer cement in shallow and medium cavities no protection was performed except for the respective adhesive system used in each group each adhesive system and resin composite was placed according to the manufacturers instructions one week later the restorations were finishedpolished and evaluated according to the usphs modified criteria all patients attended the oneyear recall and the restorations were evaluated at that time based on the same DUE criteria the scores were submitted to statistical analysis chisquare test p solitaire and tph showed some fractures at marginal ridges solitaire alert and tph showed some concerns related to color match and surface texture surefil and filtek p showed an excellent clinical performance after one year
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the lightinduced response of a neutral photoactive yellow protein chromophore in a hydrogenbonded complex with a proton acceptor has been studied by dualdetection action absorption spectroscopy and DFT we show that the chromophore is a photoacid and that ultrafast excitedstate PT might be operative in an isolated complex
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the endosperm is at the center of successful seed formation in flowering plants being itself a product of fertilization it is devoted to nourish the developing embryo and typically possesses a triploid genome consisting of two maternal and one PWS genome complement interestingly endosperm OD is controlled by epigenetic mechanisms conferring parentoforigindependent effects that influence seed development in the model plant arabidopsis thaliana we have previously described an endospermspecific heterochromatin fraction which increases with higher maternal but not PWS genome dosage here we report a detailed analysis of chromosomal arrangement and association frequency in endosperm nuclei we found that centromeric fish signals in isolated nuclei show a planar alignment that may results from a semirigid connective structure between chromosomes importantly we found frequent pairwise association of centromeres chromosomal segments and entire arms of chromosomes in c endosperm nuclei these associations deviate from random expectations predicted by numerical simulations therefore we suggest a nonrandom chromosomal organization in the triploid nuclei of arabidopsis endosperm this contrasts with the prevailing random arrangement of chromosome territories in somatic nuclei based on observations on a series of nuclei with varying parental genome ratios we propose a MM where chromosomes associate pairwise involving one maternal and one PWS complement the functional implications of this predicted chromosomal arrangement are discussed
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accumulating evidence suggests that bcell SD therapy by rituximab may be ERP for autoimmune disorders however an optimal dose of rituximab and a mechanism of its action remain to be established we performed a doseescalation study for treatment of japanese patients with AD including eight with sle and one with evans syndrome
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superoxide dismutase glutathione peroxidase and glutathione reductase activities were measured in erythrocytes of young patients with insulindependent diabetes and HS with similar age and sex CSD the diabetic patients were divided into CG and subgroups according to sex age duration of disease existence of diabetes complications and family PH of atherogenic risks data analysis was performed by comparing enzyme activities in subgroups according to the degree of diabetes control and the plasmatic level of various lipid fractions results showed that superoxide dismutase glutathione peroxidase and glutathione reductase MICs in young diabetic patients were similar to those in controls except for patients with retinopathy whose glutathione peroxidase activity was decreased this last finding might suggest that there is a relationship between the OD of diabetic microvascular complications and the accumulation of free radicals and peroxide lipids
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atherothrombosis thrombus formation superimposed on an existing atherosclerotic PI is an acute process leading to ischaemic events such as myocardial infarction stroke and critical limb ischaemia patients presenting with clinical conditions associated with atherothrombosis are at increased risk of subsequent vascular events the beneficial effect of antiplatelet therapies for shortterm and LT AA prevention of atherothrombotic events has been established these guidelines aim to provide evidencebased recommendations that will assist in the antiplateletmediated AA prophylaxis of V1 events in patients with SD cardiovascular disease treated in the primary healthcare setting medline and the cochrane library were accessed using freetext strategies in the domains of antiplatelet agents and antithrombotics development of the guidelines was driven by a series of steering committee meetings in which the quality of relevant studies was assessed and identified using narrative summary these guidelines present evidence and recommendations for the treatment of numerous atherothrombotic indications depending on individual patient circumstances
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shoulder internal rotation enhances symptom provocation attributed to cubital tunnel syndrome we present a modified elbow flexion testthe shoulder internal rotation elbow flexion testfor diagnosing cubital tunnel syndrome
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myocarditis occasionally is related to arrhythmogenic right ventricular dysplasia arvd and sometimes overlaps during the early stages which may lead to misdiagnosis acute myocarditis may reflect an active phase of arvd
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effects of cd on the AS transport of na in soringers solution on the ABD skin of a bullfrog rana catesbeiana were investigated the short circuit current scc potential difference pd and na flux were determined the skin resistance rm the electromotive force of AS na transport ena and the resistance to the AS na current rna were calculated from the measured data the application of cd to the epidermal side at mm was more ERP in increasing scc than that to the dermal side at the same concentration the time course of changes in scc induced by cd were classified into three types i a simple increase in scc during cd application ii an early decrease followed by an increase in scc and iii a decrease in scc except for a small increase in the initial short period type i response was observed in the majority of cases about twothirds types ii and iii were observed in and percent of cases respectively the cdinduced changes in scc were analyzed on the basis of changes in ena and rna in type i the increase in scc was due to a remarkable decrease in rna without an appreciable NC in ena since scc enarna in types ii and iii the changes in scc in the early and the later stages could be explained by the differences in the extents to which ena and rna decreased the exp and calculated ratios of pdcd to pdcontrol in these stages were in good agreement with each other
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neonatal and weaner gulf coast native native lambs were studied to determine whether an immunological basis underlies their NK resistance to haemonchus contortus infection neonatal native lambs n and weaner native lambs n were randomly assigned to a treatment or a control group lambs in the treatment group received dexamethasone by intramuscular i.t. three times a week for weeks neonatal and weeks weaners all lambs were monitored for fecal FEC fec blood packed cell volume pcv and WG blood cell differential counts on a weekly basis for the duration of the studies neonatal lambs were kept on pasture with their dams and weaner lambs were dewormed at weaning and kept in pens where they received trickle infections serum antibody titers to h contortus whole worm antigen wwa were determined using elisa lymphocyte proliferation assays on IP blood MNC cells pbmc were done to assess lymphocyte function all lambs were vaccinated with killed brucella abortus CS to assess the effect of dexamethasone treatment on antibody response all lambs were necropsied at the end of each T0 to recover the contents of the gastrointestinal tract for nematode enumeration and identification the results showed that mean fec and mean pcv of the TG was significantly higher and lower respectively than in the control group in both neonatal and weaner lambs from weeks and respectively at necropsy total nematode count was significantly higher in treatment groups than in the control CG serum antibody titers to h contortus wwa were significantly lower in treated CG than in control CG treatment CG showed a consistent depression in lymphocyte percentage being significantly lower from week in both neonatal and weaner lambs no differences were found in the response of pbmc to mitogen stimulation between the CG lambs in the control groups showed strong positive brucellosis card tests and the treatment CG did not dexamethasone treatment resulted in depression of the immune response and loss of natural resistance of native lambs to h contortus infection the results of these studies suggest that some aspects of the immune response may underlie the natural resistance of native sheep to h contortus infection
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we reconfirm the performance of the initial scheme for calculating the groundstate pair density higuchi and higuchi physica b phys rev b by using the alternative approximation of the correlating kinetic energy functional it is shown that about of the rho energy can be reproduced by the initial scheme irrespective of the approximate form of the correlating kinetic energy PET on the basis of the initial scheme various kinds of schemes that go beyond the initial one can be developed we illustrate two kinds of computational schemes
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the total number of circulating leucocytes in the peripheral blood of pregnant women progressively increased with advance in gestation because of neutrophilia when the phagocytic activity representing nonspecific immunity was estimated by a luminoldependent CL cl response during phagocytosing OPZ we observed that the cl response of whole blood and ficollpaque separated polymorphonuclear leucocytes pmnl significantly increased throughout the pregnancy p less than the cl responses of MNC leucocytes mn and monocytes also increased and reached peak levels in the third trimester p less than these findings suggest that the phagocytic activity in Pr women increases not only with regard to the number of phagocytes but also with regard to individual cell function from a relatively early stage of the pregnancy and that this increased nonspecific immunity may compensate in part for a weakened specific immunity of the maternal host attention should be directed to effects of human chorionic gonadotropin hcg relative to the increased cl response during pregnancy
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the possible antiallodynic effect of phosphodiesterase inhibitor sildenafil and nitric oxide donor glyceryl trinitrate as well as the changes in phosphodiesterase a mrna expression in dorsal root ganglion and spinal cord of allodynic diabetic rats was assessed diabetes was induced by streptozotocin mgkg ip in male WR streptozotocin i.t. produced hyperlglycemia polydipsia polyphagia and polyuria as well as longterm tactile allodynia weeks and a reduction of phosphodiesterase a mrna expression in spinal cord of diabetic rats SVR administration of sildenafil mgkg ip reduced tactile allodynia in a dosedependent manner in diabetic rats likewise GTN patches mgh also reduced tactile allodynia in diabetic rats moreover both drugs reversed STZ phosphodiesterase a mrna expression reduction our results indicate that GTN and sildenafil reduce tactile allodynia in diabetic rats suggesting that nitric oxide and cyclic gmp supply is an important step in their mechanism of action of these drugs in diabetic animals data suggest that nitric oxide donors as GTN and drugs which increase cyclic gmp levels as sildenafil could have a role in the pharmacotherapy of tactile allodynia in diabetic patients
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we present the case of a patient in whom coronary angiography performed due to severe calcific aortic stenosis revealed crossing between the left anterior descending artery and the first diagonal branch there is only a single report presenting this particular coronary anatomy whereas this is the eleventh case of crossing CAs ever reported the clinical implications of this variant coronary anatomy with regard to diagnostic angiography and selection of revascularization procedures are briefly discussed
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in routine tcm sulfur colloid sc liverspleen imaging relatively increased splenic uptake when compared with liver uptake is frequently seen commonly this is due to decreased hepatic uptake AA to MLD but often it is because of increased uptake in the hyperfunctioning SP reported is a case of lymphoma of the tonsil with a hot spleen which was free of tumor possible etiology of the hot SP is discussed and the known causes listed according to their prevalence
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some effects of subthreshold sinewave transcutaneous ENS tens passed between earlobe electrodes at a constant alternating current ac frequency of Hz hz were investigated in normal subjects T3 minutes of treatment and T3 T2 of standardized MS MA which immediately followed the minute treatment in a doubleblind protocol five CG received AS tens during treatment and AS tens during stress active tens during treatment and PL tens during AS placebo tens during treatment and PL tens during stress placebo tens during treatment and AS tens during AS and no treatment during both treatment and stress results showed significant reductions in systolic blood pressure PP rate and anxiety but not in diastolic blood pressure or IP vascular tension T3 T2 of AS tens as compared to no treatment no placebo tens effect was observed no significant differences were observed between active tens PL tens and no treatment in physiological or psychological response to the AS procedure results are discussed in terms of the applicability of this technique to the management of stress
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we have characterized the in vitro selfsplicing of intron ai alpha containing precursor rna from the yeast mitochondrial gene coding for cytochrome oxidase S1 i this intron follows the rules for group i selfsplicing introns and all the characteristic products have been identified in addition we have detected abnormal rna products with features that indicate that the selfsplicing behaviour of this intron is more complex two intron circles are formed by use of a L1 and minor introninternal site for circle closure a cryptic splice site located in the exon results in guanosine nucleotide mediated opening at a position nt downstream of the normal splice site the reactions can all be explained on the basis of the splice guide model proposed by davies et al nature although the CS motifs at cyclization and splice CS occur more often in this intron only some of them are allowed to interact with the internal guide sequence suggesting that both primary structure and spatial folding of the rna are involved in formation of productive reaction CS
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changes in the cochlear microphonics cm AEP nerve action potential ap and evoked responses from the IC icer and AEP cortex acer of the guinea pig were assessed T3 exposure to WN of db for min both continuous and intermittent ms noise and ms pause exposures were used in comparison with the preexposure level cm isopotential curves were shifted by db means se on the average in the range of khz recorded at the round window the amplitudeintensity CF of the clickevoked auditory NAP decreased by db that of the IC evoked response by db and the amplitudeintensity function of the auditory cortex evoked potential decreased by db a similar reduction in the amplitude was found after both continuous and intermittent noise SE in contrast to the decrease in amplitudes of evoked potentials the latencyintensity functions of the individual waves of potentials evoked along the auditory pathway did not change when compared at the same click intensity before and T3 the exposure the results suggest that individual AEP nuclei are impaired by the noise to different extents and that the impairment does not increase linearly up to the auditory cortex
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a new hplc method using charged aerosol detection was developed for the determination of terpene lactones in a ginkgo leaf extract the linearity of the standard curves was excellent r the repeatability of the method was less than and its reproducibility was less than for each analyte the limit of PCD was between and microgml the developed method was applied to the analysis of terpene lactones in ginkgo leaf products distributed in the japanese market the results suggest that some health food products contained approximately equivalent amounts of terpene lactones to those in the medical product and that the proportion of terpene lactones varied in each health product
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in an exotic reassortant orbivirus epizootic hemorrhagic disease virus serotype ehdv strain IN was first detected in the united states to characterize the reassortment configuration of this virus and to conclusively determine the parental virus of each rna segment the complete genome of ehdv IN was sequenced in addition to the genomes of representative ehdv and ehdv isolates based on genomic comparisons to all other ehdv serotypes we determined that ehdv indiana originated from a reassortment event between the australian prototype CS of ehdv csiro and the north american topotype of ehdv alberta additionally phylogenetic analysis of all ehdv indiana isolates detected in the united states from to suggests that the virus may be undergoing continual reassortment with ehdv alberta ilated the SF-36 of the hpi axis in rainbow trout our results suggest altered cortisol dynamics and reduced target tissue response to this steroid in subordinate fish while the higher transcript C2 for steroid biosynthesis in dominant fish leads us to propose an adaptive role for responding to subsequent stressors
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sudden unexpected death in epilepsy sudep is a significant cause of death for people with epilepsy recent research suggests that multiple AF may contribute and that both cardiac and respiratory mechanisms are involved both human and animal data suggest that specific genetic AF might play a role in some cases recent animal data suggest that serotonin might affect respiratory mechanisms and may be involved both cardiac and respiratory CA are more likely with generalized tonicclonic seizures uncontrolled epilepsy particularly with GTC seizures appears to be the most highly associated modifiable risk factor for sudep
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