nopperl/pmc-image-text · Datasets at Hugging Face

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0.522527	da628d26eb80443d9de90a1fd09a1ba1	Kaplan-Meier survival curves of patients treated with 2-AEH2F.	PMC9315353	fvets-09-898077-g0001.jpg
0.378817	e10c32dcd620437abd7e1a094ff8e3a8	Flowchart of the study. BMI, body mass index; FBG, fasting blood glucose; TC, total cholesterol; TG, triglycerides; LDL-C, low-density lipoprotein cholesterol; HDL-C, high-density lipoprotein cholesterol; NAFLD, non-alcoholic fatty liver disease.	PMC9315371	fnut-09-930316-g0001.jpg
0.497918	394ad70e44db426fa85ed23b37d1d8bf	Correlation between salt intake, dietary diversity, and NAFLD. (A) Univariate analysis of salt intake and NAFLD; (B) Univariate analysis of dietary diversity and NAFLD; (C) Univariate analysis of salt intake and dietary diversity. IQR, interquartile range; DDS, dietary diversity scores; NAFLD, non-alcoholic fatty liver disease; Insufficient refers to a score of 0–3, Moderate refers to a score of 4–6 and Sufficient refers to a score of 7–9; P < 0.05; **P < 0.001.	PMC9315371	fnut-09-930316-g0002.jpg
0.426012	16c42f86ac3341f9b612bae83ed2a0c0	Subgroup analysis of the salt intake, dietary diversity, and NAFLD. (A) Association of salt intake with NAFLD after adjusting for other confounding variables; (B) Association of dietary diversity with NAFLD after adjusting for other confounding variables. NAFLD, non-alcoholic fatty liver disease; Insufficient refers to a score of 0–3, Moderate refers to a score of 4–6, and Sufficient refers to a score of 7–9; P < 0.05; P < 0.01; **P < 0.001.	PMC9315371	fnut-09-930316-g0003.jpg
0.456074	225b58a97d734fb19dbb46d84d12646f	Diagram of the synthesis of GS FexOy-NPs (green) and CS FexOy-NPs (red).	PMC9315626	nanomaterials-12-02449-g001.jpg
0.456499	db11a4b493964982b4adc92140cf1c60	(A) Water loss as a function of drying time (t) at T100 °C. (B) Maximum water loss percentage, WL(∞), and kinetic parameter (k) as a function of drying temperature.	PMC9315626	nanomaterials-12-02449-g002.jpg
0.393154	c7724b335da3442fa89c420564ed4102	XRD spectra of the GS FexOy-NPs (JCPDS standard) using ethanolic (A) and aqueous (B) extracts obtained at different drying temperatures. The profile CS FexOy-NPs is included for comparison.	PMC9315626	nanomaterials-12-02449-g003.jpg
0.461345	68e86dddc5884bd7b49bbddf4553e95d	FTIR spectra of the GS FexOy-NPs using ethanolic or aqueous extracts obtained at different drying temperatures: 25 °C (A), 50 °C (B), 100 °C (C) and 150 °C (D). The profile of CS FexOy-NPs is included for comparison.	PMC9315626	nanomaterials-12-02449-g004.jpg
0.428802	c71e4bdd373b4031a88dff25ec0cd3c9	Scanning electron microscopy (SEM) images of (1) GS FexOy-NPs using ethanolic extracts: (a) T25 °C, (b) T 50 °C, (c) T 100 °C and (d) T 150 °C and aqueous extracts: (a) T25 °C, (b) T 50 °C, (c) T 100 °C and (d) T 150 °C and (2) CS FexOy-NPs.	PMC9315626	nanomaterials-12-02449-g005.jpg
0.422247	69b2b99fef9b46e9896d37dee30cbec9	Particle size distribution of GS FexOy-NPs using ethanolic extracts: (a) T25 °C, (b) T 50 °C, (c) T 100 °C and (d) T 150 °C and aqueous extracts: (a) T25 °C, (b) T 50 °C, (c) T 100 °C and (d) T 150 °C. The profile of CS FexOy-NPs is included for comparison.	PMC9315626	nanomaterials-12-02449-g006.jpg
0.421298	8d1c784c462b4ee09222b6fb4b9bfacc	Performed rhinoplasty procedure.	PMC9317161	ojac060_fig1.jpg
0.404439	7e9d73f488c648c29f60331f772088db	Type of presurgical approach in nose previously injected with hyaluronidase.	PMC9317161	ojac060_fig2.jpg
0.472589	c17555743de1493c9da2c4f44babe5dc	Waiting time between hyaluronidase infiltration and rhinoplasty.	PMC9317161	ojac060_fig3.jpg
0.428056	0df4c8d5005f465494d40f4d68be0208	Molecular characterization of sweet potato IbIPUT1 protein. (A) Comparison of complete IPUT1 protein sequences in different species. The IPUT1 sequences included IbIPUT1 from sweet potato, AtIPUT1 from Arabidopsis (AT5G18480), ZmIPUT1 (GRMZM2G166903) from Zea mays, OsIPUT1 (LOC_Os02g41520) from Oryza sativa, SlIPUT1 (Solyc05g055040 and Solyc04g009920) from Solanum lycopersicum, VvIPUT1 (GSVIVG01023535001) from Vitis vinifera and PtIPUT1 (Potri 013G049100) from Populus trichocarpa. (B) Phylogenetic trees of IbIPUT1 and its homologs in Arabidopsis.	PMC9317492	genes-13-01140-g001.jpg
0.436315	7649f9e86d894cf1afaf71d358edab62	Overexpression of IbIPUT1 influences cellular Na+ homeostasis in sweet potato roots. (A) Schematic of IbIPUT1 expression cassette and representative images showing the IbIPUT1-transgenic roots (TRs) and non-transgenic adventitious roots (ARs) in the same seedling. Quantification of the transgenic rate of the vector in Zi 8 (n = 4). (B) The relative expression of IbIPUT1 in transgenic roots (TRs) and adventitious roots (ARs). Internal reference: GAPDH. (C) Na+ accumulation in elongation root zone of ARs and TRs, as visualized through CoroNaTM Green (200 mM NaCl for 24 h). Bar = 0.3 mm. (D) Quantification of Na+ fluorescent intensity in (C) by using the Image-Pro Plus 6.0 software. Columns labeled with “***” indicate significant difference at p < 0.0001.	PMC9317492	genes-13-01140-g002.jpg
0.446663	ea5ff8fb682d4d6999df3e7621004d43	Effects of NaCl (200 mM) stress on steady-state Na+ flow in different regions of root tips of transgenic roots (TRs) and adventitious roots (ARs) of IbIPUT1. (A) Net Na+ flux measurement via NMT in adventitious roots (ARs) and IbIPUT1-transgenic roots (TRs). The steady-state Na+ flux was measured from the meristem (300, 400 and 500 µm from the tip), elongation (1.5, 2.0 and 2.5 mm from the tip) and mature (10, 12 and 15 mm from the tip) root zones, respectively, after 24 h of NaCl treatment (200 mM). Each measuring point is equivalent to the mean of at least 20 roots collected from 10 individual transgenic positive seedlings. (B) The histogram shows the average rate of net Na+ flux in the meristematic zone, elongation zone and mature zone. The error bar represents the standard error of the average value.	PMC9317492	genes-13-01140-g003.jpg
0.475839	8bbcfcc7db694dae8de33825d9ad6fd0	Overexpression of IbIPUT1 inhibits Na+ uptake in sweet potato roots. (A) Transient Na+ influx upon treatment with 20 mM NaCl was measured from the elongation root zone (2 mm from the tip) of adventitious roots (ARs) and IbIPUT1-transgenic roots (TRs) through NMT. n = 8 from four seedlings. (B) Peak Na+ influx rate in (A). Columns labeled with “*” indicate significant difference between ARs and TRs at p < 0.05. (C) Transient Na+ flux upon 20 mM NaCl was measured via NMT from the elongation root zone (2 mm from the tip) of ARs and DsRed-transgenic roots (n = 7). (D) Peak Na+ influx rate in (C).	PMC9317492	genes-13-01140-g004.jpg
0.484716	7c89464cd80348d7bf0cfe2306274985	The effect of IbIPUT1 overexpression on the NaCl-induced Ca2+ kinetics in the elongation zone of sweet potato. Transient kinetics of Ca2+ flux upon treatment with 150 mM NaCl was measured from the elongation root zone (1 mm from the tip) of TRs and ARs through NMT. n = 8.	PMC9317492	genes-13-01140-g005.jpg
0.405032	9074b858da734fc695822e8dfd0104fc	Pictures (A–F) Diagnostic imaging. (A) A polyp in the gallbladder shown in the ultrasound examination of the abdomen. (B) Hepatic steatosis of the liver shown in the ultrasound examination of the abdomen. (C) Enlarged liver shown in the abdominal CT scan with contrast. (D) Hepatic steatosis shown in the abdominal CT scan with contrast. (E) A polyp in the gallbladder shown in the abdominal CT scan with contrast. (F) Homogeneous intraluminal signal of the lumen of the portal vein shown in the abdominal CT scan with contrast.	PMC9318073	medicina-58-00896-g0A1.jpg
0.42163	2a01c609875e448b975db4fe2c4de365	Pictures (a–g): Histopathological images of the patient’s liver. (a) Hematoxylin and eosin stain: Extensive mixed-cells infiltration bundant mixed-cells infiltration with a predominance of lymphocytes and plasma cells in the portabiliary area, periportal areas and intrahepatic trabecula. (b) Selective stain: paS (+). (c) Selective stain: glycogen (+). (d) Selective stain: Masson’s trichrome (+). (e) Immunohistochemical reaction LCA (+) in the inflammatory infiltrates. (f) Immunohistochemical reaction IgG (+). (g) Immunohistochemical reaction IgM (+).	PMC9318073	medicina-58-00896-g0A2.jpg
0.426323	7c88ff257dfd4a86bc29db62a2a3b2fb	Processes by which amorphous silica is synthesized.	PMC9318389	nanomaterials-12-02392-g001.jpg
0.40933	21afd646090b4d7a81c21262282c5a72	Article-selection flow chart.	PMC9318389	nanomaterials-12-02392-g002.jpg
0.480731	b76b5818d0ba4c4084bd265500af0100	Mass loss values of the thermally treated spruce wood samples according to the time–temperature function: ML–T = (0.050·eT/37.310 − 0.378) · t/(0.874 + t); R2 = 0.957.	PMC9318417	jof-08-00739-g001.jpg
0.497672	b8803e2b8c75441980657ad0b6356ec2	Correlation between the mass loss of wood resulting from its individual thermal treatments (ML–T) and the mass loss of wood caused by decaying fungi at 8-week mycological test by fungus brown-rot S. lacrymans (ML–SL).	PMC9318417	jof-08-00739-g002.jpg
0.444862	aa3b148ffce04a5bb6468187aa7430f0	Correlation between the mass loss of wood resulting from its individual thermal treatments (ML–T) and the mass loss of wood caused by decaying fungi at 8–week mycological test by white-rot fungus T. versicolor (ML–TV).	PMC9318417	jof-08-00739-g003.jpg
0.466666	1f1da15edc5b4a1f95bf84fa6adc53bc	The percent content of wood components thermally treated spruce wood according to the mass loss during thermal treatment (Correlation equations are listed in Table 1).	PMC9318417	jof-08-00739-g004.jpg
0.424767	5d1b0dec444049b28436eaf7084e0c1c	Percentage contents of the individual hemicelluloses in thermally treated wood. Note: ARA = arabinose, GAL = galactose, MAN = mannose, XYL = xylose and GLC = glucose.	PMC9318417	jof-08-00739-g005.jpg
0.414107	39cd38d128a64a65adbaa8ab3b3fb6f9	The percent content of wood components of the thermally–fungally attacked spruce wood according to the mass loss caused by S. lacrymans (Correlation equations are listed in Table 2).	PMC9318417	jof-08-00739-g006.jpg
0.3658	e6b35bb934c3460baeb30be9b62b25d8	The percent content of wood components of the thermally–fungally attacked spruce wood according to the mass loss caused by T. versicolor (Correlation equations are listed in Table 3).	PMC9318417	jof-08-00739-g007.jpg
0.402646	9987964c35754413bd87ce3a017dfbd9	Percentage contents of the individual hemicelluloses in thermally–fungally attacked spruce wood by S. lacrymans. Note: ARA = arabinose, GAL = galactose, MAN = mannose, XYL = xylose and GLC = glucose.	PMC9318417	jof-08-00739-g008.jpg
0.413364	b37035d1bfec4780b9c28f9113458ff1	Percentage contents of the individual hemicelluloses in thermally–fungally attacked spruce wood by T. versicolor. Note: ARA = arabinose, GAL = galactose, MAN = mannose, XYL = xylose and GLC = glucose.	PMC9318417	jof-08-00739-g009.jpg
0.510907	f7bc9a1353944ba1bbf7a8eec7a0b483	The interrelationship between choline, B vitamins, amino acids, and important neurotransmitters in ASD.	PMC9318435	nutrients-14-02896-g001.jpg
0.44325	2aebf075462a4386946ab9ddcbf0459f	The life cycle of Pentastiridius leporinus. Development from the egg to the adult imago proceeds through five instar nymphs (N1–N5).	PMC9319317	insects-13-00656-g001.jpg
0.450494	a821305207824fee9bee2a6b0abeb102	Growth rate of Pentastiridius leporinus. The body length (shown as lateral length in μm on the y-axis) was determined for n = 60 nymphs over a period of 34 weeks. Light gray intervals around molting time points (dark gray bars) represent ± one standard error of the mean. A linear regression yielded the function y = 20.6x + 1176.4, which is displayed as a solid black line with gray dashed lines indicating the 95% confidence interval.	PMC9319317	insects-13-00656-g002.jpg
0.438572	afe21b6254fb4e9bb071ab7c356db530	Host choice experiment in which Pentastiridius leporinus nymphs were placed in the central area and given a free choice of three alternative hosts. The number of nymphs found in the four areas of the test was counted after 1, 3, 6, 12, 24 and 48 h. The box plot shows the distribution of nymphs in all four areas averaged over all time points. Data were analyzed using a Friedman test. Boxes span the 25th to 75th percentiles, and the horizontal line in the box represents the median. The whiskers and outliers are plotted using Tukey’s method. Outliers are plotted as individual points. Each experiment was carried out with five sets of 30 nymphs. Statistical significance: **** p ≤ 0.0001; ns, not significant.	PMC9319317	insects-13-00656-g003.jpg
0.54307	231607d6dc97431982bd4619eee08013	Subterranean Pentastiridius leporinus nymph movement. The topsoil temperature at which individual nymphs between November 2020 and March 2022 were found is plotted against the soil depth. In June, the site was changed due to the emergence of P. leporinus adults. Sample sizes per group: 0–10 cm (n = 95), 10–20 cm (n = 298), 20–30 cm (n = 95). Each data point represents one nymph. Data were analyzed by one-way ANOVA. Violin plots indicate the frequency distribution of the nymph movement; lines indicate median and quartiles. Statistical significance: **** p ≤ 0.0001; ns, not significant.	PMC9319317	insects-13-00656-g004.jpg
0.428764	d51c70984290431985356f7746a61ba5	Adult abundance (lines) and infection rates (columns) of adult P. leporinus at four different locations in Rhineland-Palatinate and Hesse. The numbers representing adult abundance are means of three sticky traps for (A) 2020 and (B) 2021. The number of adult samples per week for the analysis of SBRars + SBRps infection rates was (A) 10–40 for 2020 and (B) 7–40 for 2021.	PMC9319317	insects-13-00656-g005.jpg
0.457806	b1655e6fdce8416099fd90d6a0ad0f4e	Analysis of Pentastiridius leporinus adults by qRT-PCR for the prevalence of the SBR pathogens Candidatus Arsenophonus phytopathogenicus (SBRars) and Candidatus Phytoplasma solani (SBRps) in 2020 (left) and 2021 (right). The number of samples tested varied per site, but 349 adults in total were tested in 2020 and 296 in 2021.	PMC9319317	insects-13-00656-g006.jpg
0.462477	e2e6c84872464d2cb1b69a031f6f86b7	Oil micrograph of the Gram-negative strain TAW-CT127 grown marine 2216 Luria–Bertani agar at 28 °C for 72 h.	PMC9319644	microorganisms-10-01441-g001.jpg
0.424297	6913bc56feef4d56840844f6a832a0cf	Optimal growth conditions for the strain TAW-CT127. (a) Growth of strain TAW-CT127 at different temperatures. (b) Growth of strain TAW-CT127 at different pH. (c) Growth of strain TAW-CT127 at different salinities. Values with completely different superscript letters in the same column are significantly different from each other, p < 0.05, and error bars indicate the standard error of the mean in triplicate experiments (using Tamhan correction).	PMC9319644	microorganisms-10-01441-g002.jpg
0.425881	b1537cc9cdb244278620e907abfa054b	Phylogenetic trees constructed from the 16S rRNA sequences of strain TAW-CT127 and some other strains by the neighbor-joining method. The bold captions are the strain in this study.	PMC9319644	microorganisms-10-01441-g003.jpg
0.54536	c15a12f380714fbdb6ae4379f660140a	SEM images of strain TAW-CT127 in different media after 5 d culture. (a) SEM image of strain TAW-CT127 in the marine 2216 L–B medium. (b) SEM image of strain TAW-CT127 in the MMC medium.	PMC9319644	microorganisms-10-01441-g004.jpg
0.420448	37637c273e424a79b86a71dc93b35f3f	Degradation efficiency of BDE-209 by strain TAW-CT127 at 3 g/L (wet weight), 28 °C, pH 7.4 ± 0.2, 160 rpm for 5 d, *: p < 0.05.	PMC9319644	microorganisms-10-01441-g005.jpg
0.461664	235677f4d8a542d8ac656d017dd13c1c	Total ion chromatogram of BDE-209 degrading culture (TAW-CT127) extract.	PMC9319644	microorganisms-10-01441-g006.jpg
0.399736	8bcfaa2efeb54546a18c2b88241694fa	COG functional classification of strain TAW-CT127.	PMC9319644	microorganisms-10-01441-g007.jpg
0.39977	dd5ebef485894c90867d7f92b7d61788	Chlorocyclohexane and chlorobenzene degradation in TAW-CT127.	PMC9319644	microorganisms-10-01441-g008.jpg
0.456614	dd53855e7ed54afc940faf5ba566a5d4	Production and biotinylation of A33. (A) DNAMAN software comparison of the homology of amino acid sequences of A33 extracellular region from VARV, VACV, MPXV, and ECTV. (B) Scheme of the expression vector. (C) Expression of A33 in BL21(DE3) cells induced with IPTG. Lane 1: control BL21(DE3) cells (vector). Lane 2, 3, 4, 5, 6: different clones of BL21(DE3) cells transfected with pET/A33. (D) Purification of A33. Lane 1: 1 mg/ mL BSA for comparison; Lane 2: purified and refolded A33 protein. (E) Western blot detection of A33 biotinylation. Lane 1: A33 protein; Lane 2: biotinylated A33 protein. (F) Biotinylated A33 was recognized by anti-ECTV sera and anti-VACV sera. Sera from naive mice were used as a negative control.	PMC9319751	vaccines-10-01084-g001.jpg
0.473254	5b762d83b9584c35b672b1fe3e9b7781	Functional identification of ScFvs. (A) ELISA plates were coated with A33 and then incubated with the indicated scFvs (left panel); ELISA plates were coated with A33, PR8, VSV, or BSA and then incubated with H2 scFv (right panel). (B) ELISA plates were coated with A33, ECTV, or VACV, and then incubated with serial dilutions of H2 scFv. Uncoated wells were used as a negative control (NC). (C) Representative SPR sensorgrams of H2 scFv binding to A33. (D) Comet-inhibition assay. * p < 0.05, p < 0.01, * p < 0.001.	PMC9319751	vaccines-10-01084-g002.jpg
0.438523	8e644ed0b98745dba210bd1180c1079d	Purification and functional identification of H2 IgG. (A) SDS-PAGE of the purified H2 IgG under reducing (left) and non-reducing conditions (right). (B) The wells of ELISA plates were respectively coated with A33, VSV, PR8, or BSA and then incubated with serial dilutions of H2 IgG. (C) The wells of ELISA plates were coated with A33, ECTV, or VACV, respectively, and then incubated with serial dilutions of H2 IgG. (D) Comet-inhibition assay. * p < 0.05, p < 0.01, * p < 0.001. (E) Expression of VARV A33 in BL21 (DE3) cells induced with IPTG. Lane 1, 2, 3, 4, 5: different clones of BL21 (DE3) cells transfected with pET/VARV A33. Lane 6: control BL21 (DE3) cells (vector). (F) Purification of VARV A33. Lane 1: purified and refolded VARV A33 protein. (G) The wells of ELISA plates were coated with VARV A33 and then incubated with serial dilutions of H2 IgG. (H) Expression of MPXV A33 in BL21 (DE3) cells induced with IPTG. Lane 1: control BL21 (DE3) cells (vector). Lane 2, 3, 4, 5: different clones of BL21 (DE3) cells transfected with pET/MPXV A33. (I) Purification of MPXV A33. Lanes 1 and 2: purified and refolded MPXV A33 protein. (J) The wells of ELISA plates were coated with MPXV A33 and then incubated with serial dilutions of H2 IgG.	PMC9319751	vaccines-10-01084-g003.jpg
0.456318	eb535fda844b44fe9df75f011173e3f7	Prophylactic and therapeutic protection in mice by H2 IgG. Female BALB/c mice were infected i.p. with 5 × 106 PFU VACV. H2 IgG was administered in mice (22 mg/kg) at the indicated time points. (A) Schematic diagram of prophylactic treatment with H2 IgG in mice. (B) Weight change following VACV infection. (C) Survival curves following VACV infection. (D) Schematic diagram of treatment with H2 IgG in mice. (E) Weight change following VACV infection. (F) Survival curves following VACV infection. Data are from at least two independent experiments, with 3–5 mice per group in each experiment. * p < 0.05, p < 0.01, * p < 0.001.	PMC9319751	vaccines-10-01084-g004.jpg
0.413042	4b9a638985e1400b9a4207cc5c169257	H2 IgG promotes anti-VACV T cell and Ab responses. (A) Indicated mice were administered with H2 IgG or PBS before VACV infection. The mice were euthanized at 7 dpi and the size of the spleens was observed (left panel). Total lymphocytes per spleen were counted (right panel). (B) Left panel: flow-cytometry analysis showing the proportion of CD4+ T cells in the spleen and the proportion of IFN-γ+ cells among CD4+ T cells. FSA: forward scatter amplitude. Stained as indicated. Right panel: total CD4+ T cells per spleen and proportion of IFN-γ+ cells among CD4+ T cells. (C) Left panel: flow-cytometry analysis showing the proportion of CD8+ T cells in the spleen and the proportion of IFN-γ+ cells among CD8+ T cells. Stained as indicated. Right panel: total CD8+ T cells per spleen and proportion of IFN-γ+ cells among CD8+ T cells. Numbers indicate the proportion in the nearest quadrant. (D) Virus titers in spleens and livers were determined at 7 dpi. (E) H&E stains of the liver (original magnification, ×20) at 7 dpi. The arrow pointed to the lymphocyte-infiltration area. (F) anti-VACV IgM or IgG Ab responses were determined at 1 week and 3 weeks pi. Data are from at least two independent experiments, with 3 mice per group in each experiment. * p < 0.05, p < 0.01, * p < 0.001.	PMC9319751	vaccines-10-01084-g005.jpg
0.478172	7117fbb6af824b9db77b57d6b62b5b78	Study flow diagram.	PMC9319994	curroncol-29-00352-g001.jpg
0.358769	75846ac860464819a46249814d3af52f	Genomic co-alterations identified (N = 1395 patients).	PMC9319994	curroncol-29-00352-g002.jpg
0.436626	1dc37160b8794154a1769882488993a8	Incremental clinical benefit from use of 15-gene panel versus single gene testing.	PMC9319994	curroncol-29-00352-g003.jpg
0.459098	01ab010a396b4ff99eebcb0fc560c5f5	Impact of sodium acetate on body weight (a) and ovarian weight (b) in LET-induced PCOS rat model. Data are expressed as mean ± SD. n = 6. Data were analyzed by one-way ANOVA followed by Bonferroni post hoc test. (*p<0.05 VS. CTL; #p<0.05 VS. PCOS). Control (CTL); Polycystic ovarian syndrome (PCOS); Sodium acetate (NaAc).	PMC9321379	pone.0272124.g001.jpg
0.46139	73c7a277292341ae82f20316c4e5c0a8	Impact of sodium acetate on the histomorphology of ovaries in LET-induced PCOS rat model.The photomicrographs of ovarian tissues show ovarian tissue with normal follicles and normal antrum, granulosa cells, thecal cells and oocyte in control animals (CTL), ovarian tissue with normal follicles and normal granulosa cells, thecal cells, oocyte and large antrum in NaAc-treated animals (NaAc), ovarian tissue with degenerated follicles and disrupted granulosa cells, thecal cells, antrum and oocyte in PCOS animals (PCOS) and ovarian tissue with preserved follicles and normal granulosa cells, thecal cells, oocyte and large antrum in PCOS+NaAc-treated animals; (H & E paraffin stain; transverse section; Scale bar 51 μm). Oocyte (O); Antrum (A); Thecal cells (T); Granulosa cell (ZG). Data are expressed as mean ± SD. n = 6. Data were analyzed by one-way ANOVA followed by Bonferroni post hoc test. (*p<0.05 VS. CTL; #p<0.05 VS. PCOS). Control (CTL); Polycystic ovarian syndrome (PCOS); Sodium acetate (NaAc).	PMC9321379	pone.0272124.g002.jpg
0.533003	cbdb5a7a879a46b7b85d8de02b2df1c1	Impact of sodium acetate on fasting blood glucose (a), 1-hour postload glucose (b), fasting insulin (c) and insulin sensitivity (d) in LET-induced PCOS rat model. Data are expressed as mean ± SD. n = 6. Data were analyzed by one-way ANOVA followed by Bonferroni post hoc test. (*p<0.05 VS. CTL; #p<0.05 VS. PCOS). Control (CTL); Polycystic ovarian syndrome (PCOS); Sodium acetate (NaAc); Quantitative check for insulin sensitivity (QUICKI).	PMC9321379	pone.0272124.g003.jpg
0.438484	826ef430af6e47e4ad92e73c96f57d10	Impact of sodium acetate on plasma and ovarian triglyceride (a, b), total cholesterol (c, d) and free fatty acid (e, f) in LET-induced PCOS rat model. Data are expressed as mean ± SD. n = 6. Data were analyzed by one-way ANOVA followed by Bonferroni post hoc test. (*p<0.05 VS. CTL; #p<0.05 VS. PCOS). Control (CTL); Polycystic ovarian syndrome (PCOS); Sodium acetate (NaAc); Free fatty acid (FFF).	PMC9321379	pone.0272124.g004.jpg
0.558762	c246a581297340feacc95adcce5247c3	Impact of sodium acetate on ovarian malondialdehyde (a), glutathione peroxidase (b), reduced glutathione (c) and Nrf2 (d) in LET-induced PCOS rat model. Data are expressed as mean ± SD. n = 6. Data were analyzed by one-way ANOVA followed by Bonferroni post hoc test. (*p<0.05 VS. CTL; #p<0.05 VS. PCOS). Control (CTL); Polycystic ovarian syndrome (PCOS); Sodium acetate (NaAc); nuclear factor erythroid-derived 2–like 2 (Nrf2).	PMC9321379	pone.0272124.g005.jpg
0.542123	6dc0f6e205984fc6abc21f34c499604f	Impact of sodium acetate on plasma and ovarian histone deacetylase (a, b) and tumor necrosis factor-α (c, d) in LET-induced PCOS rat model. Data are expressed as mean ± SD. n = 6. Data were analyzed by one-way ANOVA followed by Bonferroni post hoc test. (*p<0.05 VS. CTL; #p<0.05 VS. PCOS). Control (CTL); Polycystic ovarian syndrome (PCOS); Sodium acetate (NaAc); Histone deacetylase (HDAC); Tumor necrosis factor-α (TNF- α).	PMC9321379	pone.0272124.g006.jpg
0.607666	976715231eb74baba9244c9d470e3196	How virtual assistants function taking into account the recognized emotions.	PMC9321989	sensors-22-05311-g001.jpg
0.382269	31f96ac59e084e5faf9bbb986286fa27	Beak color and sex correlation analysis (a); population structure analysis (b).	PMC9322730	genes-13-01271-g001.jpg
0.405415	1a948b5ef20a4d79b14f774466557c9a	Quantile–quantile (Q–Q) from GWAS for beak color trait in duck. Q–Q plot showing the late separation between observed and expected values. The red lines indicate the null hypothesis of no true association. Deviation from the expected p-value distribution is evident only in the tail area for each trait, indicating that population stratification was properly controlled. BB refers to black beak; BS refers to spotted beak; BY refers to yellow beak.	PMC9322730	genes-13-01271-g002.jpg
0.452802	50a604f893434be385c4d0305f23cec5	Manhattan plots showing the significance of genetic effects on the beak color according to the GWAS.	PMC9322730	genes-13-01271-g003.jpg
0.555753	39c123ee445e496da614fa791b73b026	Venn analysis of all beak colors showing overlap of significant SNPs.	PMC9322730	genes-13-01271-g004.jpg
0.433453	6dc0bd5cc5034cf8a0e47d1bd363c131	Functional enrichment analysis of the beak color candidate genes. (a) KEGG (left) and GO (right) enrichment of black beak candidate genes; (b) KEGG (left) and GO (right) enrichment of spotted beak candidate genes; (c) KEGG (left) and GO (right) enrichment of yellow beak candidate genes.	PMC9322730	genes-13-01271-g005.jpg
0.464947	6dfbbf8b5a5e4913bbbdac57d5d45573	Expression differences in EDNRB2 and MITF on three exon junctions between black and yellow beaks according to RT-qPCR. (a) Information on the MITF isoform. The red triangle represents the intronic insertion on chromosome 13 in Pekin ducks. Exon 1M is specific for the MITF-M transcript, while exon 1B is specific for the MITF-B transcript. (b) EDNRB2 and MITF on three exon junctions between black and yellow beaks. Each exon junction was assayed in six biological replicates with three technical replicates. The indicated p-values were based on one-way ANOVA. NS, nonsignificant; **, extremely significant.	PMC9322730	genes-13-01271-g006.jpg
0.437845	c55277b087fe412fb4cc59cfd163301e	FESEM representative images of bare NPs (a) and CaPCa-NPs (b); EDS maps of the region shown in (b) for Cr, Cl, P, Ca, Na, O, and C (c). Images were collected at 10 kV with the standard SE detector and 15 kV with the in-beam SE detector. Instrumental magnification: 30,000× and 10,000×.	PMC9322757	pharmaceutics-14-01362-g001.jpg
0.40557	5c52408218e9461487423a7da58ae02d	C12DOXO release profiles from 0.1% w/w Tween®80 aqueous solution, NP6, and CaPCa-NP6.	PMC9322757	pharmaceutics-14-01362-g002.jpg
0.389663	9d71183af5d44526a774459305c5bc0e	U-2OS and U-2OS/DX cells were incubated with NP0, CaPP-NP0, and CaPCa-NP0, diluted 1:10 (1000 µg/mL trilaurin) in the cell culture medium, for 24, 48 and 72 h. Cell viability was measured using the MTT assay. Results are means ± SD (n = 3).	PMC9322757	pharmaceutics-14-01362-g003.jpg
0.412516	17b2359fd53641e39add01531049b17c	U-2OS (a) and U-2OS/DX (b) cells were incubated with NP6, CaPP-NP6, and CaPCa-NP6, carrying 0.1, 0.5, 1, 2.5, or 5 µg/mL C12DOXO for 24, 48, and 72 h. Cell viability was measured using the MTT assay. Results are means ± SD (n = 3). U-2OS, 24 h: p < 0.05 for CaPP-NP6 (1 µg/mL); p < 0.001 for C12DOXO, NP6, CaPP-NP6, and CaPCa-NP6 (2.5 and 5 µg/mL). U-2OS, 48 h: p < 0.05 for C12DOXO, NP6, CaPP-NP6, and CaPCa-NP6 (all concentrations); p < 0.001 for C12DOXO, NP6, CaPP-NP6, and CaPCa-NP6 (all concentrations). U-2OS, 72 h: p < 0.01 for C12DOXO (0.5 µg/mL); p < 0.001 for C12DOXO, NP6, CaPP-NP6, and CaPCa-NP6 (all concentrations). U-2OS/DX, 24 h: p < 0.05 for NP6 (5 µg/mL); p < 0.01 for NP6, CaPP-NP6, and CaPCa-NP6 (5 µg/mL). U-2OS/DX, 48 h: p < 0.05 for NP6 (2.5 µg/mL) and CaPCa-NP6 1 µg/mL); p < 0.001 for NP6 (5 µg/mL), CaPP-NP6, and CaPCa-NP6 (2.5 and 5 µg/mL). U-2OS/DX, 72 h: p < 0.01 for NP6, CaPP-NP6, and CaPCa-NP6 (1 µg/mL); p < 0.001 for NP6, CaPP-NP6, and CaPCa-NP6 (2.5 and 5 µg/mL).	PMC9322757	pharmaceutics-14-01362-g004.jpg
0.396705	a93eb042b7f34c1f9219d0d9619faaff	U-2OS (a) and U-2OS/DX (b) cells were incubated with 5 µg/mL C12 DOXO or with NP6, CaPP-NP6, and CaPCa-NP6, carrying 5 µg/mL C12DOXO, for 1, 3, 6, and 24 h. Intracellular retention of DOXO was measured spectrofluorimetrically. Results are means + SD (n = 3). U-2OS, 6 h: p < 0.01 for C12DOXO, NP6, and CaPP-NP6; p < 0.001 for CaPCa-NP6. U-2OS, 6 h: p < 0.001 for C12DOXO, NP6, CaPP-NP6, and CaPCa-NP6 (all parameters compared to 1 h). CaPP-NP6 vs. C12DOXO, NP6, and CaPP-NP6: p < 0.01 at 6 h; p < 0.001 at 24 h. U-2OS/DX, 6 h: p < 0.01 for CaPP-NP6; p < 0.001 for CaPCa-NP6. U-2OS/DX, 24 h: p < 0.05 for NP6, p < 0.01 for CaPP-NP6; p < 0.001 for CaPCa-NP6 (all parameters compared to 1 h). For NP6 vs. C12DOXO: p < 0.05 at 6 and 24 h. CaPP-NP6 and CaPP-NP6 vs. C12DOXO and NP6: p < 0.001 at 6 h and 24 h.	PMC9322757	pharmaceutics-14-01362-g005.jpg
0.487635	77855294b3b84309a2d1b76dcf2859b0	D17 cells were incubated with NP0, CaPP-NP0, and CapCa-NP0, diluted 1:10 (1000 µg/mL trilaurin) in the cell culture medium, for 24, 48, and 72 h. Cell viability was measured using the MTT assay. Results are means + SD (n = 0.3).	PMC9322757	pharmaceutics-14-01362-g006.jpg
0.409069	979dbe3a1a6a44788470b0550ef00764	D17 cells were incubated with NP6, CaPP-NP6, and CaPCa-NP6, carrying 0.1, 0.5, 1, 2.5, or 5 µg/mL C12DOXO, for 24, 48, and 72 h. Cell viability was measured using the MTT assay. Results are means + SD (n = 3).	PMC9322757	pharmaceutics-14-01362-g007.jpg
0.393176	9ada3c66bc194cd8a97d14e002244302	D17 cells were incubated with 5 µg/mL C12DOXO or with NP6, CaPP-NP6, and CaPCa-NP6, carrying 5 µg/mL C12DOXO, for 1, 3, 6, and 24 h. Intracellular retention of DOXO was measured spectrofluorimetrically. Results are means ± SD (n = 3).	PMC9322757	pharmaceutics-14-01362-g008.jpg
0.449132	342320298393456cbb59e9904b166aae	GC-MS chromatogram of Sf.Cr. The sample was injected at 220 °C having the scanning range of 70–700 m/z and compounds were identified by comparing retention time and mass fragmentation using NIST 2014 mass spectral library.	PMC9322968	molecules-27-04368-g001.jpg
0.437126	4f997ad50bf742babbe28faf11883a0e	Representative images showing ethanol-induced gastric ulceration in rat’s stomach where arrowheads indicate macroscopic lesions and petechiae. (a) Normal control group showing normal morphology, (b) intoxicated group showing widespread hemorrhagic lesions, (c) sucralfate (100 mg/kg) pre-treatment group showing few lesions and spot ulcers, (d) 30 mg/kg, (e) 100 mg/kg, and (f) 300 mg/kg Sf.Cr pre-treatment group showing dose-dependent gastroprotection.	PMC9322968	molecules-27-04368-g002.jpg
0.420692	a007c83b85fa4e7aa20c1e403e536399	Effect of Sf.Cr on ulcer index. Rats were pre-treated orally with normal saline, sucralfate, and Sf.Cr. Data are presented as Mean ± SEM (n = 6). Significance was determined by One way ANOVA followed by Dunnett’s test and described as (***) if p < 0.001 compared with the intoxicated animals.	PMC9322968	molecules-27-04368-g003.jpg
0.41998	ce04bd4b507e4b0094f0c2929dc1753d	Effect of Sf.Cr on gastric mucus contents. Values are expressed as Mean ± SEM. Significance was determined by one way ANOVA followed by Dunnett’s test. Values are considered as significant () if p < 0.05, highly significant (**) if p < 0.001 compared with intoxicated group, and (##) if p < 0.01, (###) p < 0.001 compared with normal control group.	PMC9322968	molecules-27-04368-g004.jpg
0.46958	b33608a923b34fa1a6f199454eadf2e9	Histomicrographs of the rat stomach with H & E stain at magnification of 400X (scale bar: 100 µm). Control group (a) shows normal morphology and no visible signs of inflammation; intoxicated group (b) showing several inflammatory changes including bloody spots (arrow), oedema (arrowhead), and polymorphic nuclear cell infiltration (*). The stomach mucosa and submucosa showed fewer sings of inflammation when treated with sucralfate however infiltration and bloody spots are visible (c) and Sf.Cr showed amelioration of stomach tunics in a dose dependent manner with inconspicuous inflammatory changes (d–f).	PMC9322968	molecules-27-04368-g005.jpg
0.437965	63656e7bb3674a6b94d786b06b586313	Amphiphilic AuNPs and amphiphilic peptides: similarities between their spontaneous penetration mechanism into lipid membranes. (a) Structure of an amphiphilic MUS:OT AuNP with its coarse-grained (CG) representation (2:1 MUS:OT ligand ratio). Red beads represent hydrophobic carbon groups, while green beads represent the charged MUS terminals. (b) Different stages of penetration of a MUS:OT AuNP into a 1-palmitoyl-2-oleoyl-glycero-3-phosphocholine (POPC) lipid bilayer obtained from CG molecular dynamics simulations. From left to right: adsorbed state, hydrophobic contact state, and snorkeling of the first MUS ligand which binds to the opposite leaflet. Eventually—through a sequential anchoring process—more and more MUS ligands are dropped leading to the fully snorkeled configuration of the NP-membrane complex. Lipid heads are blue (choline) and tan (phosphate), lipid tails and water are not shown. (c) Translocation process of an amphiphilic ‘Spontaneous Membrane-Translocating Peptide’ (SMTP) into a POPC lipid bilayer obtained from united atom bias-exchange metadynamics simulations. Specifically, the SMTP contains a LRLLR sequence composed of two Arg (R) and three leucines (L) residues. From left to right: SMTP located in the lipid head region, SMTP on its way towards the opposite leaflet, and final snorkeled configuration. The first Arg is shown in cyan, the second Arg is shown in red, and leucine hydrophobic residues are shown in green. Nitrogen and phosphorus atoms in the lipid head region are shown in blue and yellow, respectively. The lipid tails are shown as thin gray lines, while water is shown as red (oxygen) and gray (hydrogen) cylinders. (a,b) adapted with permission from Simonelli et al. [38]—Copyright © 2015 American Chemical Society. (c) reprinted with permission from Cao et al. [51]—Copyright © 2020 Elsevier B.V. All rights reserved.	PMC9324301	membranes-12-00673-g001.jpg
0.416931	dfb07b20ffaf415899a2066c8ad1d5f2	Amphiphilic AuNPs and amphiphilic peptides: a common affinity for the disordered domains of phase−separated lipid membranes. (a) Phase−separated SLBs containing 1,2-dioleoyl-sn-glycero-3-phosphocholine (DOPC), sphingomyelin (SM), cholesterol and ganglioside GM1 (63:31:1:5 molar ratio) imaged in liquid by atomic force microscopy (AFM) after addition of ~3 nm MUS:OT AuNPs (40 min and 15 h). After hours, large clusters of amphiphilic AuNPs (white arrows) slowly formed on the darker disordered phase and at the edges of the lighter (i.e., higher) ordered lipid domains. (b) Potential of mean force (PMF) profiles calculated for the adsorption of a single MUS:OT AuNPs on the surface of the Ld and Lo phase. The Ld phase, with a binding free energy of ~18 kJ/mol (~9 kBT), is favoured over the Lo phase (~11 kJ/mol, ~5 kBT). (c) Giant plasma membrane vesicles (GPMVs) derived from rat basophilic leukemia cells incubated at low temperature with three examples of fluorescein-labeled CPPs—i.e., MAP (model amphipathic peptide), penetratin (pAntp) and transportan 10 (TP10) (green). All these CPPs are amphipatic and contain Lys or Arg residues. Lo and Ld phases are labeled with AF594-labeled cholera toxin B subunit (CtxB, red) and AF647-labeled annexin V (AnV, pseudocolored as white), respectively. (a,b) contain images by Canepa et al. [44] reprinted with minor modifications under a CC BY-NC 3.0 license with permission from the Royal Society of Chemistry. (c) is reproduced and adapted with permission from Säälik et al. [81]—Copyright © 2011 Elsevier B.V. All rights reserved.	PMC9324301	membranes-12-00673-g002.jpg
0.440193	97bea8bef09d40d8af374c749b9ed027	Perturbation of membrane ordered–disordered phase separation upon interaction with amphiphilic AuNPs and amphiphilic peptides. (a) Topographic AFM images showing fragmentation of ordered domains induced by ~3 nm MUS:OT AuNPs on phase-separated lipid bilayers containing DOPC:SM:chol:GM1 (63:31:1:5 molar ratio). Two height profiles of the phase-separated membrane without NPs are also reported. On the right: comparison of height difference distributions (Δz) between ordered and disordered domains before and after NP/membrane interaction. (b) Fluorescence images of the same region of a POPC:1,2-dimyristoyl-sn-glycero-3-PG (DMPG) 1:1 phase-separated SLB with PG-enriched ordered domains recorded before and after exposure to the lipopetide DAP (1 μM). Two solid ordered domains (dark regions) in a liquid disordered background (bright region) containing the fluorescence lipid probe DHPE-Texas Red (TR, 1%) are shown. The channel of kynurenine (KYN)—an intrinsically fluorescent DAP residue—is used to visualize the morphology of ordered domains since DAP strongly interacts with PG lipids. Overall, the SLB/DAP interaction induces an extensive size reduction of the solid domains equal to 59%. (c) Force spectroscopy analysis and topographic AFM images of the same POPC:DMPG 1:1 SLB before and after exposure to increasing DAP concentrations (0–8 μM). Jump-through (J–T) force maps (resolution 32 × 32 pixels) are reported next to each AFM image, together with the comparison of solid and fluid domains jump-through force upon increasing concentrations of DAP. (a) contains images by Canepa et al. [44] reprinted with minor modifications under a CC BY-NC 3.0 license with permission from the Royal Society of Chemistry. (b,c) are reproduced and adapted with permission from Mescola et al. [99]—Copyright © 2020 American Chemical Society. All rights reserved.	PMC9324301	membranes-12-00673-g003.jpg
0.438654	bde5e2e432394a43833a07cd6fcc33d9	Translocation of amphiphilic AuNPs and amphiphilic peptides is favoured into lipid membranes with lower cholesterol content. (a) Left: coarse-grained structure of hydrophilic (MUS) and hydrophobic (OT) ligands and an amphiphilic MUS:OT AuNP in water (2 nm core size; water not shown). Right: simulation snapshots showing the ligand anchoring typical of these AuNPs (see Figure 1b). The NP goes from the hydrophobic contact state (top) to the anchored state (bottom) in which one MUS charged terminal is in contact with the lipid heads (transparent gray) of the distal leaflet. Cholesterol molecules—intercalated between the apolar tails of membrane phospholipids (DOPC)—are shown in tan in the membrane detail on the bottom left. (b) Anchoring free energy barriers calculated with well-tempered metadynamics simulations at different membrane cholesterol concentrations. (c) Average anchoring time (Δtanchor) and average number of anchored ligands after 1 μs (Nanchors) obtained from unbiased MD simulations as a function of membrane cholesterol content. (d) Translocation of the Arg-rich CPP nona-arginine (Arg9)—labeled with fluorescein (green)—into GPMVs derived from MDA-MB-231 (MDA GPMV) and RBL-2H3 (RBL GPMV) cells. Left images: GPMVs labeled with filipin (pseudo-colored as white) to bind membrane cholesterol and enable its visualization. Right images: GPMVs labeled with Alexa Fluor 555-conjugated cholera toxin B subunit (CtxB, red) and Alexa Fluor 647-conjugated annexin V (AnV, pseudocolored as white) to visualize, respectively, the Lo membrane domains and phosphatidylserine contained in the outer leaflet of the limiting membrane of both vesicle types. Quantification of the filipin signal shows that MDA GPMVs contain approximately 30% less membrane cholesterol than RBL GPMVs; in addition, RBL GPMVs show several large cholesterol-enriched subdomains (white arrows) that are rarer in MDA GPMVs. Overall, Arg9 translocation is significantly reduced in vesicles characterized by higher membrane cholesterol content and more cholesterol-rich membrane microdomains. *** p-value < 0.0001 and 0.0005 for filipin signal and fluo-Arg9 translocation, respectively. (a–c) contain images by Canepa et al. [45] reprinted with minor modifications—Copyright © 2021 The Authors, published by American Chemical Society. (d) is reproduced and adapted with permission from Lorents et al. [111]—Copyright © 2018 American Chemical Society.	PMC9324301	membranes-12-00673-g004.jpg
0.419943	b1beba99207f4a14940cd84e097850af	The tendency for membrane aggregation is shared by amphiphilic AuNPs and amphiphilic peptides. (a) Top, sketch of two curvature inducing membrane inclusions; due to the elastic energy of the membrane, an effective interacting potential can arise and, depending on the system, the interaction can be attractive and thus lead to aggregation. Bottom, system in which the inclusions suppress the natural fluctuations of the membrane; aggregation can minimize this region. (b) Aggregation induced by lipid depletion. (c) Aggregation induced by capillary forces. In configuration (1), there are two regions of modified lipid density around the inclusion; dimerization allows to minimize their total area, as shown in configuration (2). (d) Ordered aggregate of MUS:OT NPs adsorbed on a DOPC membrane. The snapshot is taken from unbiased MD simulations (Martini CG). Nanoparticles are represented with yellow beads (Au), pink beads (S), cyan (MUS ligands) and blue (OT ligands); membrane lipids are represented with red beads. (e) Dimer of adsorbed NPs on DOPC membrane from unbiased MD simulations (Martini CG). The extended ligand configuration can be observed. Representation as in (d). (f) Cryo-EM image of MUS:OT aggregation on the surface of a DOPC liposome. The NP-NP is compatible with the extended ligand configuration. (g) Ordered aggregate of MUS:OT NPs embedded in a model neuronal plasma membrane. The snapshot is taken from unbiased MD simulations (Martini CG). The NP are represented with a yellow core, blue OT ligands and cyan MUS ligands. The membrane is represented with red DliPC lipids, light pink sphingomyelin, yellow ganglioside and grey cholesterol. (h) Dimer of MUS:OT NPs embedded in a model neuronal plasma membrane. The deformation of the ligand shell and the presence of the stabilizing layer of ions (red beads) can be observed. The membrane headgroups are shown as semi-transparent surface, lipid tails are not shown for clarity. (i) Supramolecular lattice formed by M1 bilayer-embedded MUS:OT NPS, imaged by AFM. The digital zoom of the area with blue contour shows the lattice order at higher magnification. (a–c) adapted with permission from Johannes et al. [152] Copyright © 2022 Elsevier B.V. All rights reserved. (d–f) adapted from Lavagna et al. [173] with permission from the Royal Society of Chemistry. (g–i) adapted from Canepa et al. [44] under a CC BY-NC 3.0 license with permission from the Royal Society of Chemistry.	PMC9324301	membranes-12-00673-g005.jpg
0.475652	8d7c89a4943642c0a1fa50b5da1264a1	Pathways affected by COPD status [16].	PMC9324381	metabolites-12-00621-g001.jpg
0.433705	09ab333216e84dc3ab13883239497c8f	Inclusion/Exclusion Flowchart.	PMC9324381	metabolites-12-00621-g002.jpg
0.411665	a4b1bc8bae5d499e80ceb3ba9321ea8c	Cumulative incidence function for composite outcome (death, stroke, systemic embolism, gastrointestinal, or intra-cranial haemorrhage) by frailty category and time-varying anticoagulation status (with 95% confidence intervals).	PMC9326851	euac022f1.jpg
0.418244	fa5b85c5311e44e9b86e47a8f2e7c33d	Cumulative incidence function for all-cause death by frailty category and time-varying anticoagulation status (with 95% confidence intervals).	PMC9326851	euac022f2.jpg
0.405207	6cc4e99943bf4616a285ddce2331837a	Cumulative incidence function for stroke by frailty category and anticoagulation status (with 95% confidence intervals).	PMC9326851	euac022f3.jpg
0.392181	5b4b4b995780421c95b9daad8d595252	Cumulative incidence function for severe bleeding by frailty category and anticoagulation status (with 95% confidence intervals).	PMC9326851	euac022f4.jpg
0.448668	6d4ff7e3d3424474b12e523b9ea0b96f	Cumulative incidence function for transient ischaemic attack by frailty category and anticoagulation status.	PMC9326851	euac022f5.jpg
0.42629	04d2c3e2b4674fbabc9cba06d63346bc	High blood lead (Pb) level (BLL) locations identified with Getis-Ord Gi* geospatial cluster analysis for exceedance rate of EBLLs (≥5μg/dL) in census tracts and reference locations (2014–2016; children 0 to <6 years of age; 2,401 total census tracts evaluated). See also Table 2. Note: EBLL, elevated blood lead level.	PMC9327739	ehp9705_f1.jpg
0.451622	74294d9111d347499e060a0787d466b7	Getis-Ord Gi* geospatial cluster analysis for exceedance rate of elevated blood lead (Pb) levels (≥5μg/dL) in census tracts by years (children 0 to <6 years of age; number of census tracts evaluated: (A) 2006 to 2013, 2,400; (B) 2011 to 2013, 2,405; (C) 2014 to 2016, 2,401. See also Table 3.	PMC9327739	ehp9705_f2.jpg
0.471004	2373dcfeb39e48d6962e1e5bc8213336	Getis-Ord Gi* geospatial cluster analysis for exceedance rate of elevated blood lead (Pb) levels [(A) ≥5μg/dL; (B) ≥10μg/dL] in census tracts by previous Centers for Disease Control and Prevention (CDC) reference values (2014–2016; children 0 to <6 years of age; 2,401 census tracts evaluated). See also Table 3.	PMC9327739	ehp9705_f3.jpg
0.430574	8b437f3ee02f44498753ca7562dabe31	Changes over time for exceedance rate of %EBLLs by census tracts—lower peninsula of Michigan (number of census tracts evaluated: (A) 2006–2007, 2,244; (B) 2008–2010, 2,383; (C) 2011–2013, 2,405; (D) 2014–2016, 2,401). See also Table 1 and Table S1. Note: %EBLLs, percentage elevated blood lead levels.	PMC9327739	ehp9705_f4.jpg
0.470399	d9e19f9932ce4ff99e817bdd7281fd37	Getis-Ord Gi* geospatial cluster analysis for (A) EJSCREEN 2017 Pb Paint EJ Index (2,752 census tracts evaluated), (B) Schultz et al. regression model13 approach BLL Data (2,774 census tracts evaluated), and (C) HUD Deteriorated Paint Index (2,741 census tracts evaluated) displayed side by side with (D) the Getis-Ord Gi* cluster analysis for exceedance rate of EBLLs (2,401 census tracts evaluated; ≥5μg/dL; 2014–2016; children 0 to <6 years of age). See also Table 3. Note: BLL, blood lead level; EBLL, elevated blood lead level; EJ, environmental justice; EPA, Environmental Protection Agency; HUD, U.S. Department of Housing and Urban Development; Kappa, Cohen’s Kappa agreement statistic (0.41–0.6, moderate; 0.61–0.8, substantial; 0.81–0.99, near perfect agreement); Pb, lead.	PMC9327739	ehp9705_f5.jpg
0.449596	72bfcd8003d6491f9685eeaaad1a966d	The process of content analysis	PMC9327824	HEX-25--g001.jpg
0.49116	783c582b138d416190903a48266b1fff	Timeline of NCCN APP Workgroup and surveys.	PMC9328455	jadpro-13-507-g001.jpg
0.412938	a03306d732d843588083c8b7240c3681	Survey question on RVUs as an indicator of APP productivity (N = 489).	PMC9328455	jadpro-13-507-g002.jpg

0.522527

da628d26eb80443d9de90a1fd09a1ba1

Kaplan-Meier survival curves of patients treated with 2-AEH2F.

PMC9315353

fvets-09-898077-g0001.jpg

0.378817

e10c32dcd620437abd7e1a094ff8e3a8

Flowchart of the study. BMI, body mass index; FBG, fasting blood glucose; TC, total cholesterol; TG, triglycerides; LDL-C, low-density lipoprotein cholesterol; HDL-C, high-density lipoprotein cholesterol; NAFLD, non-alcoholic fatty liver disease.

PMC9315371

fnut-09-930316-g0001.jpg

0.497918

394ad70e44db426fa85ed23b37d1d8bf

Correlation between salt intake, dietary diversity, and NAFLD. (A) Univariate analysis of salt intake and NAFLD; (B) Univariate analysis of dietary diversity and NAFLD; (C) Univariate analysis of salt intake and dietary diversity. IQR, interquartile range; DDS, dietary diversity scores; NAFLD, non-alcoholic fatty liver disease; Insufficient refers to a score of 0–3, Moderate refers to a score of 4–6 and Sufficient refers to a score of 7–9; *P < 0.05; ***P < 0.001.

PMC9315371

fnut-09-930316-g0002.jpg

0.426012

16c42f86ac3341f9b612bae83ed2a0c0

Subgroup analysis of the salt intake, dietary diversity, and NAFLD. (A) Association of salt intake with NAFLD after adjusting for other confounding variables; (B) Association of dietary diversity with NAFLD after adjusting for other confounding variables. NAFLD, non-alcoholic fatty liver disease; Insufficient refers to a score of 0–3, Moderate refers to a score of 4–6, and Sufficient refers to a score of 7–9; *P < 0.05; **P < 0.01; ***P < 0.001.

PMC9315371

fnut-09-930316-g0003.jpg

0.456074

225b58a97d734fb19dbb46d84d12646f

Diagram of the synthesis of GS FexOy-NPs (green) and CS FexOy-NPs (red).

PMC9315626

nanomaterials-12-02449-g001.jpg

0.456499

db11a4b493964982b4adc92140cf1c60

(A) Water loss as a function of drying time (t) at T100 °C. (B) Maximum water loss percentage, WL(∞), and kinetic parameter (k) as a function of drying temperature.

PMC9315626

nanomaterials-12-02449-g002.jpg

0.393154

c7724b335da3442fa89c420564ed4102

XRD spectra of the GS FexOy-NPs (JCPDS standard) using ethanolic (A) and aqueous (B) extracts obtained at different drying temperatures. The profile CS FexOy-NPs is included for comparison.

PMC9315626

nanomaterials-12-02449-g003.jpg

0.461345

68e86dddc5884bd7b49bbddf4553e95d

FTIR spectra of the GS FexOy-NPs using ethanolic or aqueous extracts obtained at different drying temperatures: 25 °C (A), 50 °C (B), 100 °C (C) and 150 °C (D). The profile of CS FexOy-NPs is included for comparison.

PMC9315626

nanomaterials-12-02449-g004.jpg

0.428802

c71e4bdd373b4031a88dff25ec0cd3c9

Scanning electron microscopy (SEM) images of (1) GS FexOy-NPs using ethanolic extracts: (a) T25 °C, (b) T 50 °C, (c) T 100 °C and (d) T 150 °C and aqueous extracts: (a*) T25 °C, (b*) T 50 °C, (c*) T 100 °C and (d*) T 150 °C and (2) CS FexOy-NPs.

PMC9315626

nanomaterials-12-02449-g005.jpg

0.422247

69b2b99fef9b46e9896d37dee30cbec9

Particle size distribution of GS FexOy-NPs using ethanolic extracts: (a) T25 °C, (b) T 50 °C, (c) T 100 °C and (d) T 150 °C and aqueous extracts: (a*) T25 °C, (b*) T 50 °C, (c*) T 100 °C and (d*) T 150 °C. The profile of CS FexOy-NPs is included for comparison.

PMC9315626

nanomaterials-12-02449-g006.jpg

0.421298

8d1c784c462b4ee09222b6fb4b9bfacc

Performed rhinoplasty procedure.

PMC9317161

ojac060_fig1.jpg

0.404439

7e9d73f488c648c29f60331f772088db

Type of presurgical approach in nose previously injected with hyaluronidase.

PMC9317161

ojac060_fig2.jpg

0.472589

c17555743de1493c9da2c4f44babe5dc

Waiting time between hyaluronidase infiltration and rhinoplasty.

PMC9317161

ojac060_fig3.jpg

0.428056

0df4c8d5005f465494d40f4d68be0208

Molecular characterization of sweet potato IbIPUT1 protein. (A) Comparison of complete IPUT1 protein sequences in different species. The IPUT1 sequences included IbIPUT1 from sweet potato, AtIPUT1 from Arabidopsis (AT5G18480), ZmIPUT1 (GRMZM2G166903) from Zea mays, OsIPUT1 (LOC_Os02g41520) from Oryza sativa, SlIPUT1 (Solyc05g055040 and Solyc04g009920) from Solanum lycopersicum, VvIPUT1 (GSVIVG01023535001) from Vitis vinifera and PtIPUT1 (Potri 013G049100) from Populus trichocarpa. (B) Phylogenetic trees of IbIPUT1 and its homologs in Arabidopsis.

PMC9317492

genes-13-01140-g001.jpg

0.436315

7649f9e86d894cf1afaf71d358edab62

Overexpression of IbIPUT1 influences cellular Na+ homeostasis in sweet potato roots. (A) Schematic of IbIPUT1 expression cassette and representative images showing the IbIPUT1-transgenic roots (TRs) and non-transgenic adventitious roots (ARs) in the same seedling. Quantification of the transgenic rate of the vector in Zi 8 (n = 4). (B) The relative expression of IbIPUT1 in transgenic roots (TRs) and adventitious roots (ARs). Internal reference: GAPDH. (C) Na+ accumulation in elongation root zone of ARs and TRs, as visualized through CoroNaTM Green (200 mM NaCl for 24 h). Bar = 0.3 mm. (D) Quantification of Na+ fluorescent intensity in (C) by using the Image-Pro Plus 6.0 software. Columns labeled with “***” indicate significant difference at p < 0.0001.

PMC9317492

genes-13-01140-g002.jpg

0.446663

ea5ff8fb682d4d6999df3e7621004d43

Effects of NaCl (200 mM) stress on steady-state Na+ flow in different regions of root tips of transgenic roots (TRs) and adventitious roots (ARs) of IbIPUT1. (A) Net Na+ flux measurement via NMT in adventitious roots (ARs) and IbIPUT1-transgenic roots (TRs). The steady-state Na+ flux was measured from the meristem (300, 400 and 500 µm from the tip), elongation (1.5, 2.0 and 2.5 mm from the tip) and mature (10, 12 and 15 mm from the tip) root zones, respectively, after 24 h of NaCl treatment (200 mM). Each measuring point is equivalent to the mean of at least 20 roots collected from 10 individual transgenic positive seedlings. (B) The histogram shows the average rate of net Na+ flux in the meristematic zone, elongation zone and mature zone. The error bar represents the standard error of the average value.

PMC9317492

genes-13-01140-g003.jpg

0.475839

8bbcfcc7db694dae8de33825d9ad6fd0

Overexpression of IbIPUT1 inhibits Na+ uptake in sweet potato roots. (A) Transient Na+ influx upon treatment with 20 mM NaCl was measured from the elongation root zone (2 mm from the tip) of adventitious roots (ARs) and IbIPUT1-transgenic roots (TRs) through NMT. n = 8 from four seedlings. (B) Peak Na+ influx rate in (A). Columns labeled with “*” indicate significant difference between ARs and TRs at p < 0.05. (C) Transient Na+ flux upon 20 mM NaCl was measured via NMT from the elongation root zone (2 mm from the tip) of ARs and DsRed-transgenic roots (n = 7). (D) Peak Na+ influx rate in (C).

PMC9317492

genes-13-01140-g004.jpg

0.484716

7c89464cd80348d7bf0cfe2306274985

The effect of IbIPUT1 overexpression on the NaCl-induced Ca2+ kinetics in the elongation zone of sweet potato. Transient kinetics of Ca2+ flux upon treatment with 150 mM NaCl was measured from the elongation root zone (1 mm from the tip) of TRs and ARs through NMT. n = 8.

PMC9317492

genes-13-01140-g005.jpg

0.405032

9074b858da734fc695822e8dfd0104fc

Pictures (A–F) Diagnostic imaging. (A) A polyp in the gallbladder shown in the ultrasound examination of the abdomen. (B) Hepatic steatosis of the liver shown in the ultrasound examination of the abdomen. (C) Enlarged liver shown in the abdominal CT scan with contrast. (D) Hepatic steatosis shown in the abdominal CT scan with contrast. (E) A polyp in the gallbladder shown in the abdominal CT scan with contrast. (F) Homogeneous intraluminal signal of the lumen of the portal vein shown in the abdominal CT scan with contrast.

PMC9318073

medicina-58-00896-g0A1.jpg

0.42163

2a01c609875e448b975db4fe2c4de365

Pictures (a–g): Histopathological images of the patient’s liver. (a) Hematoxylin and eosin stain: Extensive mixed-cells infiltration bundant mixed-cells infiltration with a predominance of lymphocytes and plasma cells in the portabiliary area, periportal areas and intrahepatic trabecula. (b) Selective stain: paS (+). (c) Selective stain: glycogen (+). (d) Selective stain: Masson’s trichrome (+). (e) Immunohistochemical reaction LCA (+) in the inflammatory infiltrates. (f) Immunohistochemical reaction IgG (+). (g) Immunohistochemical reaction IgM (+).

PMC9318073

medicina-58-00896-g0A2.jpg

0.426323

7c88ff257dfd4a86bc29db62a2a3b2fb

Processes by which amorphous silica is synthesized.

PMC9318389

nanomaterials-12-02392-g001.jpg

0.40933

21afd646090b4d7a81c21262282c5a72

Article-selection flow chart.

PMC9318389

nanomaterials-12-02392-g002.jpg

0.480731

b76b5818d0ba4c4084bd265500af0100

Mass loss values of the thermally treated spruce wood samples according to the time–temperature function: ML–T = (0.050·eT/37.310 − 0.378) · t/(0.874 + t); R2 = 0.957.

PMC9318417

jof-08-00739-g001.jpg

0.497672

b8803e2b8c75441980657ad0b6356ec2

Correlation between the mass loss of wood resulting from its individual thermal treatments (ML–T) and the mass loss of wood caused by decaying fungi at 8-week mycological test by fungus brown-rot S. lacrymans (ML–SL).

PMC9318417

jof-08-00739-g002.jpg

0.444862

aa3b148ffce04a5bb6468187aa7430f0

Correlation between the mass loss of wood resulting from its individual thermal treatments (ML–T) and the mass loss of wood caused by decaying fungi at 8–week mycological test by white-rot fungus T. versicolor (ML–TV).

PMC9318417

jof-08-00739-g003.jpg

0.466666

1f1da15edc5b4a1f95bf84fa6adc53bc

The percent content of wood components thermally treated spruce wood according to the mass loss during thermal treatment (Correlation equations are listed in Table 1).

PMC9318417

jof-08-00739-g004.jpg

0.424767

5d1b0dec444049b28436eaf7084e0c1c

Percentage contents of the individual hemicelluloses in thermally treated wood. Note: ARA = arabinose, GAL = galactose, MAN = mannose, XYL = xylose and GLC = glucose.

PMC9318417

jof-08-00739-g005.jpg

0.414107

39cd38d128a64a65adbaa8ab3b3fb6f9

The percent content of wood components of the thermally–fungally attacked spruce wood according to the mass loss caused by S. lacrymans (Correlation equations are listed in Table 2).

PMC9318417

jof-08-00739-g006.jpg

0.3658

e6b35bb934c3460baeb30be9b62b25d8

The percent content of wood components of the thermally–fungally attacked spruce wood according to the mass loss caused by T. versicolor (Correlation equations are listed in Table 3).

PMC9318417

jof-08-00739-g007.jpg

0.402646

9987964c35754413bd87ce3a017dfbd9

Percentage contents of the individual hemicelluloses in thermally–fungally attacked spruce wood by S. lacrymans. Note: ARA = arabinose, GAL = galactose, MAN = mannose, XYL = xylose and GLC = glucose.

PMC9318417

jof-08-00739-g008.jpg

0.413364

b37035d1bfec4780b9c28f9113458ff1

Percentage contents of the individual hemicelluloses in thermally–fungally attacked spruce wood by T. versicolor. Note: ARA = arabinose, GAL = galactose, MAN = mannose, XYL = xylose and GLC = glucose.

PMC9318417

jof-08-00739-g009.jpg

0.510907

f7bc9a1353944ba1bbf7a8eec7a0b483

The interrelationship between choline, B vitamins, amino acids, and important neurotransmitters in ASD.

PMC9318435

nutrients-14-02896-g001.jpg

0.44325

2aebf075462a4386946ab9ddcbf0459f

The life cycle of Pentastiridius leporinus. Development from the egg to the adult imago proceeds through five instar nymphs (N1–N5).

PMC9319317

insects-13-00656-g001.jpg

0.450494

a821305207824fee9bee2a6b0abeb102

Growth rate of Pentastiridius leporinus. The body length (shown as lateral length in μm on the y-axis) was determined for n = 60 nymphs over a period of 34 weeks. Light gray intervals around molting time points (dark gray bars) represent ± one standard error of the mean. A linear regression yielded the function y = 20.6x + 1176.4, which is displayed as a solid black line with gray dashed lines indicating the 95% confidence interval.

PMC9319317

insects-13-00656-g002.jpg

0.438572

afe21b6254fb4e9bb071ab7c356db530

Host choice experiment in which Pentastiridius leporinus nymphs were placed in the central area and given a free choice of three alternative hosts. The number of nymphs found in the four areas of the test was counted after 1, 3, 6, 12, 24 and 48 h. The box plot shows the distribution of nymphs in all four areas averaged over all time points. Data were analyzed using a Friedman test. Boxes span the 25th to 75th percentiles, and the horizontal line in the box represents the median. The whiskers and outliers are plotted using Tukey’s method. Outliers are plotted as individual points. Each experiment was carried out with five sets of 30 nymphs. Statistical significance: **** p ≤ 0.0001; ns, not significant.

PMC9319317

insects-13-00656-g003.jpg

0.54307

231607d6dc97431982bd4619eee08013

Subterranean Pentastiridius leporinus nymph movement. The topsoil temperature at which individual nymphs between November 2020 and March 2022 were found is plotted against the soil depth. In June, the site was changed due to the emergence of P. leporinus adults. Sample sizes per group: 0–10 cm (n = 95), 10–20 cm (n = 298), 20–30 cm (n = 95). Each data point represents one nymph. Data were analyzed by one-way ANOVA. Violin plots indicate the frequency distribution of the nymph movement; lines indicate median and quartiles. Statistical significance: **** p ≤ 0.0001; ns, not significant.

PMC9319317

insects-13-00656-g004.jpg

0.428764

d51c70984290431985356f7746a61ba5

Adult abundance (lines) and infection rates (columns) of adult P. leporinus at four different locations in Rhineland-Palatinate and Hesse. The numbers representing adult abundance are means of three sticky traps for (A) 2020 and (B) 2021. The number of adult samples per week for the analysis of SBRars + SBRps infection rates was (A) 10–40 for 2020 and (B) 7–40 for 2021.

PMC9319317

insects-13-00656-g005.jpg

0.457806

b1655e6fdce8416099fd90d6a0ad0f4e

Analysis of Pentastiridius leporinus adults by qRT-PCR for the prevalence of the SBR pathogens Candidatus Arsenophonus phytopathogenicus (SBRars) and Candidatus Phytoplasma solani (SBRps) in 2020 (left) and 2021 (right). The number of samples tested varied per site, but 349 adults in total were tested in 2020 and 296 in 2021.

PMC9319317

insects-13-00656-g006.jpg

0.462477

e2e6c84872464d2cb1b69a031f6f86b7

Oil micrograph of the Gram-negative strain TAW-CT127 grown marine 2216 Luria–Bertani agar at 28 °C for 72 h.

PMC9319644

microorganisms-10-01441-g001.jpg

0.424297

6913bc56feef4d56840844f6a832a0cf

Optimal growth conditions for the strain TAW-CT127. (a) Growth of strain TAW-CT127 at different temperatures. (b) Growth of strain TAW-CT127 at different pH. (c) Growth of strain TAW-CT127 at different salinities. Values with completely different superscript letters in the same column are significantly different from each other, p < 0.05, and error bars indicate the standard error of the mean in triplicate experiments (using Tamhan correction).

PMC9319644

microorganisms-10-01441-g002.jpg

0.425881

b1537cc9cdb244278620e907abfa054b

Phylogenetic trees constructed from the 16S rRNA sequences of strain TAW-CT127 and some other strains by the neighbor-joining method. The bold captions are the strain in this study.

PMC9319644

microorganisms-10-01441-g003.jpg

0.54536

c15a12f380714fbdb6ae4379f660140a

SEM images of strain TAW-CT127 in different media after 5 d culture. (a) SEM image of strain TAW-CT127 in the marine 2216 L–B medium. (b) SEM image of strain TAW-CT127 in the MMC medium.

PMC9319644

microorganisms-10-01441-g004.jpg

0.420448

37637c273e424a79b86a71dc93b35f3f

Degradation efficiency of BDE-209 by strain TAW-CT127 at 3 g/L (wet weight), 28 °C, pH 7.4 ± 0.2, 160 rpm for 5 d, *: p < 0.05.

PMC9319644

microorganisms-10-01441-g005.jpg

0.461664

235677f4d8a542d8ac656d017dd13c1c

Total ion chromatogram of BDE-209 degrading culture (TAW-CT127) extract.

PMC9319644

microorganisms-10-01441-g006.jpg

0.399736

8bcfaa2efeb54546a18c2b88241694fa

COG functional classification of strain TAW-CT127.

PMC9319644

microorganisms-10-01441-g007.jpg

0.39977

dd5ebef485894c90867d7f92b7d61788

Chlorocyclohexane and chlorobenzene degradation in TAW-CT127.

PMC9319644

microorganisms-10-01441-g008.jpg

0.456614

dd53855e7ed54afc940faf5ba566a5d4

Production and biotinylation of A33. (A) DNAMAN software comparison of the homology of amino acid sequences of A33 extracellular region from VARV, VACV, MPXV, and ECTV. (B) Scheme of the expression vector. (C) Expression of A33 in BL21(DE3) cells induced with IPTG. Lane 1: control BL21(DE3) cells (vector). Lane 2, 3, 4, 5, 6: different clones of BL21(DE3) cells transfected with pET/A33. (D) Purification of A33. Lane 1: 1 mg/ mL BSA for comparison; Lane 2: purified and refolded A33 protein. (E) Western blot detection of A33 biotinylation. Lane 1: A33 protein; Lane 2: biotinylated A33 protein. (F) Biotinylated A33 was recognized by anti-ECTV sera and anti-VACV sera. Sera from naive mice were used as a negative control.

PMC9319751

vaccines-10-01084-g001.jpg

0.473254

5b762d83b9584c35b672b1fe3e9b7781

Functional identification of ScFvs. (A) ELISA plates were coated with A33 and then incubated with the indicated scFvs (left panel); ELISA plates were coated with A33, PR8, VSV, or BSA and then incubated with H2 scFv (right panel). (B) ELISA plates were coated with A33, ECTV, or VACV, and then incubated with serial dilutions of H2 scFv. Uncoated wells were used as a negative control (NC). (C) Representative SPR sensorgrams of H2 scFv binding to A33. (D) Comet-inhibition assay. * p < 0.05, ** p < 0.01, *** p < 0.001.

PMC9319751

vaccines-10-01084-g002.jpg

0.438523

8e644ed0b98745dba210bd1180c1079d

Purification and functional identification of H2 IgG. (A) SDS-PAGE of the purified H2 IgG under reducing (left) and non-reducing conditions (right). (B) The wells of ELISA plates were respectively coated with A33, VSV, PR8, or BSA and then incubated with serial dilutions of H2 IgG. (C) The wells of ELISA plates were coated with A33, ECTV, or VACV, respectively, and then incubated with serial dilutions of H2 IgG. (D) Comet-inhibition assay. * p < 0.05, ** p < 0.01, *** p < 0.001. (E) Expression of VARV A33 in BL21 (DE3) cells induced with IPTG. Lane 1, 2, 3, 4, 5: different clones of BL21 (DE3) cells transfected with pET/VARV A33. Lane 6: control BL21 (DE3) cells (vector). (F) Purification of VARV A33. Lane 1: purified and refolded VARV A33 protein. (G) The wells of ELISA plates were coated with VARV A33 and then incubated with serial dilutions of H2 IgG. (H) Expression of MPXV A33 in BL21 (DE3) cells induced with IPTG. Lane 1: control BL21 (DE3) cells (vector). Lane 2, 3, 4, 5: different clones of BL21 (DE3) cells transfected with pET/MPXV A33. (I) Purification of MPXV A33. Lanes 1 and 2: purified and refolded MPXV A33 protein. (J) The wells of ELISA plates were coated with MPXV A33 and then incubated with serial dilutions of H2 IgG.

PMC9319751

vaccines-10-01084-g003.jpg

0.456318

eb535fda844b44fe9df75f011173e3f7

Prophylactic and therapeutic protection in mice by H2 IgG. Female BALB/c mice were infected i.p. with 5 × 106 PFU VACV. H2 IgG was administered in mice (22 mg/kg) at the indicated time points. (A) Schematic diagram of prophylactic treatment with H2 IgG in mice. (B) Weight change following VACV infection. (C) Survival curves following VACV infection. (D) Schematic diagram of treatment with H2 IgG in mice. (E) Weight change following VACV infection. (F) Survival curves following VACV infection. Data are from at least two independent experiments, with 3–5 mice per group in each experiment. * p < 0.05, ** p < 0.01, *** p < 0.001.

PMC9319751

vaccines-10-01084-g004.jpg

0.413042

4b9a638985e1400b9a4207cc5c169257

H2 IgG promotes anti-VACV T cell and Ab responses. (A) Indicated mice were administered with H2 IgG or PBS before VACV infection. The mice were euthanized at 7 dpi and the size of the spleens was observed (left panel). Total lymphocytes per spleen were counted (right panel). (B) Left panel: flow-cytometry analysis showing the proportion of CD4+ T cells in the spleen and the proportion of IFN-γ+ cells among CD4+ T cells. FSA: forward scatter amplitude. Stained as indicated. Right panel: total CD4+ T cells per spleen and proportion of IFN-γ+ cells among CD4+ T cells. (C) Left panel: flow-cytometry analysis showing the proportion of CD8+ T cells in the spleen and the proportion of IFN-γ+ cells among CD8+ T cells. Stained as indicated. Right panel: total CD8+ T cells per spleen and proportion of IFN-γ+ cells among CD8+ T cells. Numbers indicate the proportion in the nearest quadrant. (D) Virus titers in spleens and livers were determined at 7 dpi. (E) H&E stains of the liver (original magnification, ×20) at 7 dpi. The arrow pointed to the lymphocyte-infiltration area. (F) anti-VACV IgM or IgG Ab responses were determined at 1 week and 3 weeks pi. Data are from at least two independent experiments, with 3 mice per group in each experiment. * p < 0.05, ** p < 0.01, *** p < 0.001.

PMC9319751

vaccines-10-01084-g005.jpg

0.478172

7117fbb6af824b9db77b57d6b62b5b78

Study flow diagram.

PMC9319994

curroncol-29-00352-g001.jpg

0.358769

75846ac860464819a46249814d3af52f

Genomic co-alterations identified (N = 1395 patients).

PMC9319994

curroncol-29-00352-g002.jpg

0.436626

1dc37160b8794154a1769882488993a8

Incremental clinical benefit from use of 15-gene panel versus single gene testing.

PMC9319994

curroncol-29-00352-g003.jpg

0.459098

01ab010a396b4ff99eebcb0fc560c5f5

Impact of sodium acetate on body weight (a) and ovarian weight (b) in LET-induced PCOS rat model. Data are expressed as mean ± SD. n = 6. Data were analyzed by one-way ANOVA followed by Bonferroni post hoc test. (*p<0.05 VS. CTL; #p<0.05 VS. PCOS). Control (CTL); Polycystic ovarian syndrome (PCOS); Sodium acetate (NaAc).

PMC9321379

pone.0272124.g001.jpg

0.46139

73c7a277292341ae82f20316c4e5c0a8

Impact of sodium acetate on the histomorphology of ovaries in LET-induced PCOS rat model.The photomicrographs of ovarian tissues show ovarian tissue with normal follicles and normal antrum, granulosa cells, thecal cells and oocyte in control animals (CTL), ovarian tissue with normal follicles and normal granulosa cells, thecal cells, oocyte and large antrum in NaAc-treated animals (NaAc), ovarian tissue with degenerated follicles and disrupted granulosa cells, thecal cells, antrum and oocyte in PCOS animals (PCOS) and ovarian tissue with preserved follicles and normal granulosa cells, thecal cells, oocyte and large antrum in PCOS+NaAc-treated animals; (H & E paraffin stain; transverse section; Scale bar 51 μm). Oocyte (O); Antrum (A); Thecal cells (T); Granulosa cell (ZG). Data are expressed as mean ± SD. n = 6. Data were analyzed by one-way ANOVA followed by Bonferroni post hoc test. (*p<0.05 VS. CTL; #p<0.05 VS. PCOS). Control (CTL); Polycystic ovarian syndrome (PCOS); Sodium acetate (NaAc).

PMC9321379

pone.0272124.g002.jpg

0.533003

cbdb5a7a879a46b7b85d8de02b2df1c1

Impact of sodium acetate on fasting blood glucose (a), 1-hour postload glucose (b), fasting insulin (c) and insulin sensitivity (d) in LET-induced PCOS rat model. Data are expressed as mean ± SD. n = 6. Data were analyzed by one-way ANOVA followed by Bonferroni post hoc test. (*p<0.05 VS. CTL; #p<0.05 VS. PCOS). Control (CTL); Polycystic ovarian syndrome (PCOS); Sodium acetate (NaAc); Quantitative check for insulin sensitivity (QUICKI).

PMC9321379

pone.0272124.g003.jpg

0.438484

826ef430af6e47e4ad92e73c96f57d10

Impact of sodium acetate on plasma and ovarian triglyceride (a, b), total cholesterol (c, d) and free fatty acid (e, f) in LET-induced PCOS rat model. Data are expressed as mean ± SD. n = 6. Data were analyzed by one-way ANOVA followed by Bonferroni post hoc test. (*p<0.05 VS. CTL; #p<0.05 VS. PCOS). Control (CTL); Polycystic ovarian syndrome (PCOS); Sodium acetate (NaAc); Free fatty acid (FFF).

PMC9321379

pone.0272124.g004.jpg

0.558762

c246a581297340feacc95adcce5247c3

Impact of sodium acetate on ovarian malondialdehyde (a), glutathione peroxidase (b), reduced glutathione (c) and Nrf2 (d) in LET-induced PCOS rat model. Data are expressed as mean ± SD. n = 6. Data were analyzed by one-way ANOVA followed by Bonferroni post hoc test. (*p<0.05 VS. CTL; #p<0.05 VS. PCOS). Control (CTL); Polycystic ovarian syndrome (PCOS); Sodium acetate (NaAc); nuclear factor erythroid-derived 2–like 2 (Nrf2).

PMC9321379

pone.0272124.g005.jpg

0.542123

6dc0f6e205984fc6abc21f34c499604f

Impact of sodium acetate on plasma and ovarian histone deacetylase (a, b) and tumor necrosis factor-α (c, d) in LET-induced PCOS rat model. Data are expressed as mean ± SD. n = 6. Data were analyzed by one-way ANOVA followed by Bonferroni post hoc test. (*p<0.05 VS. CTL; #p<0.05 VS. PCOS). Control (CTL); Polycystic ovarian syndrome (PCOS); Sodium acetate (NaAc); Histone deacetylase (HDAC); Tumor necrosis factor-α (TNF- α).

PMC9321379

pone.0272124.g006.jpg

0.607666

976715231eb74baba9244c9d470e3196

How virtual assistants function taking into account the recognized emotions.

PMC9321989

sensors-22-05311-g001.jpg

0.382269

31f96ac59e084e5faf9bbb986286fa27

Beak color and sex correlation analysis (a); population structure analysis (b).

PMC9322730

genes-13-01271-g001.jpg

0.405415

1a948b5ef20a4d79b14f774466557c9a

Quantile–quantile (Q–Q) from GWAS for beak color trait in duck. Q–Q plot showing the late separation between observed and expected values. The red lines indicate the null hypothesis of no true association. Deviation from the expected p-value distribution is evident only in the tail area for each trait, indicating that population stratification was properly controlled. BB refers to black beak; BS refers to spotted beak; BY refers to yellow beak.

PMC9322730

genes-13-01271-g002.jpg

0.452802

50a604f893434be385c4d0305f23cec5

Manhattan plots showing the significance of genetic effects on the beak color according to the GWAS.

PMC9322730

genes-13-01271-g003.jpg

0.555753

39c123ee445e496da614fa791b73b026

Venn analysis of all beak colors showing overlap of significant SNPs.

PMC9322730

genes-13-01271-g004.jpg

0.433453

6dc0bd5cc5034cf8a0e47d1bd363c131

Functional enrichment analysis of the beak color candidate genes. (a) KEGG (left) and GO (right) enrichment of black beak candidate genes; (b) KEGG (left) and GO (right) enrichment of spotted beak candidate genes; (c) KEGG (left) and GO (right) enrichment of yellow beak candidate genes.

PMC9322730

genes-13-01271-g005.jpg

0.464947

6dfbbf8b5a5e4913bbbdac57d5d45573

Expression differences in EDNRB2 and MITF on three exon junctions between black and yellow beaks according to RT-qPCR. (a) Information on the MITF isoform. The red triangle represents the intronic insertion on chromosome 13 in Pekin ducks. Exon 1M is specific for the MITF-M transcript, while exon 1B is specific for the MITF-B transcript. (b) EDNRB2 and MITF on three exon junctions between black and yellow beaks. Each exon junction was assayed in six biological replicates with three technical replicates. The indicated p-values were based on one-way ANOVA. NS, nonsignificant; **, extremely significant.

PMC9322730

genes-13-01271-g006.jpg

0.437845

c55277b087fe412fb4cc59cfd163301e

FESEM representative images of bare NPs (a) and CaPCa-NPs (b); EDS maps of the region shown in (b) for Cr, Cl, P, Ca, Na, O, and C (c). Images were collected at 10 kV with the standard SE detector and 15 kV with the in-beam SE detector. Instrumental magnification: 30,000× and 10,000×.

PMC9322757

pharmaceutics-14-01362-g001.jpg

0.40557

5c52408218e9461487423a7da58ae02d

C12DOXO release profiles from 0.1% w/w Tween®80 aqueous solution, NP6, and CaPCa-NP6.

PMC9322757

pharmaceutics-14-01362-g002.jpg

0.389663

9d71183af5d44526a774459305c5bc0e

U-2OS and U-2OS/DX cells were incubated with NP0, CaPP-NP0, and CaPCa-NP0, diluted 1:10 (1000 µg/mL trilaurin) in the cell culture medium, for 24, 48 and 72 h. Cell viability was measured using the MTT assay. Results are means ± SD (n = 3).

PMC9322757

pharmaceutics-14-01362-g003.jpg

0.412516

17b2359fd53641e39add01531049b17c

U-2OS (a) and U-2OS/DX (b) cells were incubated with NP6, CaPP-NP6, and CaPCa-NP6, carrying 0.1, 0.5, 1, 2.5, or 5 µg/mL C12DOXO for 24, 48, and 72 h. Cell viability was measured using the MTT assay. Results are means ± SD (n = 3). U-2OS, 24 h: p < 0.05 for CaPP-NP6 (1 µg/mL); p < 0.001 for C12DOXO, NP6, CaPP-NP6, and CaPCa-NP6 (2.5 and 5 µg/mL). U-2OS, 48 h: p < 0.05 for C12DOXO, NP6, CaPP-NP6, and CaPCa-NP6 (all concentrations); p < 0.001 for C12DOXO, NP6, CaPP-NP6, and CaPCa-NP6 (all concentrations). U-2OS, 72 h: p < 0.01 for C12DOXO (0.5 µg/mL); p < 0.001 for C12DOXO, NP6, CaPP-NP6, and CaPCa-NP6 (all concentrations). U-2OS/DX, 24 h: p < 0.05 for NP6 (5 µg/mL); p < 0.01 for NP6, CaPP-NP6, and CaPCa-NP6 (5 µg/mL). U-2OS/DX, 48 h: p < 0.05 for NP6 (2.5 µg/mL) and CaPCa-NP6 1 µg/mL); p < 0.001 for NP6 (5 µg/mL), CaPP-NP6, and CaPCa-NP6 (2.5 and 5 µg/mL). U-2OS/DX, 72 h: p < 0.01 for NP6, CaPP-NP6, and CaPCa-NP6 (1 µg/mL); p < 0.001 for NP6, CaPP-NP6, and CaPCa-NP6 (2.5 and 5 µg/mL).

PMC9322757

pharmaceutics-14-01362-g004.jpg

0.396705

a93eb042b7f34c1f9219d0d9619faaff

U-2OS (a) and U-2OS/DX (b) cells were incubated with 5 µg/mL C12 DOXO or with NP6, CaPP-NP6, and CaPCa-NP6, carrying 5 µg/mL C12DOXO, for 1, 3, 6, and 24 h. Intracellular retention of DOXO was measured spectrofluorimetrically. Results are means + SD (n = 3). U-2OS, 6 h: p < 0.01 for C12DOXO, NP6, and CaPP-NP6; p < 0.001 for CaPCa-NP6. U-2OS, 6 h: p < 0.001 for C12DOXO, NP6, CaPP-NP6, and CaPCa-NP6 (all parameters compared to 1 h). CaPP-NP6 vs. C12DOXO, NP6, and CaPP-NP6: p < 0.01 at 6 h; p < 0.001 at 24 h. U-2OS/DX, 6 h: p < 0.01 for CaPP-NP6; p < 0.001 for CaPCa-NP6. U-2OS/DX, 24 h: p < 0.05 for NP6, p < 0.01 for CaPP-NP6; p < 0.001 for CaPCa-NP6 (all parameters compared to 1 h). For NP6 vs. C12DOXO: p < 0.05 at 6 and 24 h. CaPP-NP6 and CaPP-NP6 vs. C12DOXO and NP6: p < 0.001 at 6 h and 24 h.

PMC9322757

pharmaceutics-14-01362-g005.jpg

0.487635

77855294b3b84309a2d1b76dcf2859b0

D17 cells were incubated with NP0, CaPP-NP0, and CapCa-NP0, diluted 1:10 (1000 µg/mL trilaurin) in the cell culture medium, for 24, 48, and 72 h. Cell viability was measured using the MTT assay. Results are means + SD (n = 0.3).

PMC9322757

pharmaceutics-14-01362-g006.jpg

0.409069

979dbe3a1a6a44788470b0550ef00764

D17 cells were incubated with NP6, CaPP-NP6, and CaPCa-NP6, carrying 0.1, 0.5, 1, 2.5, or 5 µg/mL C12DOXO, for 24, 48, and 72 h. Cell viability was measured using the MTT assay. Results are means + SD (n = 3).

PMC9322757

pharmaceutics-14-01362-g007.jpg

0.393176

9ada3c66bc194cd8a97d14e002244302

D17 cells were incubated with 5 µg/mL C12DOXO or with NP6, CaPP-NP6, and CaPCa-NP6, carrying 5 µg/mL C12DOXO, for 1, 3, 6, and 24 h. Intracellular retention of DOXO was measured spectrofluorimetrically. Results are means ± SD (n = 3).

PMC9322757

pharmaceutics-14-01362-g008.jpg

0.449132

342320298393456cbb59e9904b166aae

GC-MS chromatogram of Sf.Cr. The sample was injected at 220 °C having the scanning range of 70–700 m/z and compounds were identified by comparing retention time and mass fragmentation using NIST 2014 mass spectral library.

PMC9322968

molecules-27-04368-g001.jpg

0.437126

4f997ad50bf742babbe28faf11883a0e

Representative images showing ethanol-induced gastric ulceration in rat’s stomach where arrowheads indicate macroscopic lesions and petechiae. (a) Normal control group showing normal morphology, (b) intoxicated group showing widespread hemorrhagic lesions, (c) sucralfate (100 mg/kg) pre-treatment group showing few lesions and spot ulcers, (d) 30 mg/kg, (e) 100 mg/kg, and (f) 300 mg/kg Sf.Cr pre-treatment group showing dose-dependent gastroprotection.

PMC9322968

molecules-27-04368-g002.jpg

0.420692

a007c83b85fa4e7aa20c1e403e536399

Effect of Sf.Cr on ulcer index. Rats were pre-treated orally with normal saline, sucralfate, and Sf.Cr. Data are presented as Mean ± SEM (n = 6). Significance was determined by One way ANOVA followed by Dunnett’s test and described as (***) if p < 0.001 compared with the intoxicated animals.

PMC9322968

molecules-27-04368-g003.jpg

0.41998

ce04bd4b507e4b0094f0c2929dc1753d

Effect of Sf.Cr on gastric mucus contents. Values are expressed as Mean ± SEM. Significance was determined by one way ANOVA followed by Dunnett’s test. Values are considered as significant (*) if p < 0.05, highly significant (***) if p < 0.001 compared with intoxicated group, and (##) if p < 0.01, (###) p < 0.001 compared with normal control group.

PMC9322968

molecules-27-04368-g004.jpg

0.46958

b33608a923b34fa1a6f199454eadf2e9

Histomicrographs of the rat stomach with H & E stain at magnification of 400X (scale bar: 100 µm). Control group (a) shows normal morphology and no visible signs of inflammation; intoxicated group (b) showing several inflammatory changes including bloody spots (arrow), oedema (arrowhead), and polymorphic nuclear cell infiltration (*). The stomach mucosa and submucosa showed fewer sings of inflammation when treated with sucralfate however infiltration and bloody spots are visible (c) and Sf.Cr showed amelioration of stomach tunics in a dose dependent manner with inconspicuous inflammatory changes (d–f).

PMC9322968

molecules-27-04368-g005.jpg

0.437965

63656e7bb3674a6b94d786b06b586313

Amphiphilic AuNPs and amphiphilic peptides: similarities between their spontaneous penetration mechanism into lipid membranes. (a) Structure of an amphiphilic MUS:OT AuNP with its coarse-grained (CG) representation (2:1 MUS:OT ligand ratio). Red beads represent hydrophobic carbon groups, while green beads represent the charged MUS terminals. (b) Different stages of penetration of a MUS:OT AuNP into a 1-palmitoyl-2-oleoyl-glycero-3-phosphocholine (POPC) lipid bilayer obtained from CG molecular dynamics simulations. From left to right: adsorbed state, hydrophobic contact state, and snorkeling of the first MUS ligand which binds to the opposite leaflet. Eventually—through a sequential anchoring process—more and more MUS ligands are dropped leading to the fully snorkeled configuration of the NP-membrane complex. Lipid heads are blue (choline) and tan (phosphate), lipid tails and water are not shown. (c) Translocation process of an amphiphilic ‘Spontaneous Membrane-Translocating Peptide’ (SMTP) into a POPC lipid bilayer obtained from united atom bias-exchange metadynamics simulations. Specifically, the SMTP contains a LRLLR sequence composed of two Arg (R) and three leucines (L) residues. From left to right: SMTP located in the lipid head region, SMTP on its way towards the opposite leaflet, and final snorkeled configuration. The first Arg is shown in cyan, the second Arg is shown in red, and leucine hydrophobic residues are shown in green. Nitrogen and phosphorus atoms in the lipid head region are shown in blue and yellow, respectively. The lipid tails are shown as thin gray lines, while water is shown as red (oxygen) and gray (hydrogen) cylinders. (a,b) adapted with permission from Simonelli et al. [38]—Copyright © 2015 American Chemical Society. (c) reprinted with permission from Cao et al. [51]—Copyright © 2020 Elsevier B.V. All rights reserved.

PMC9324301

membranes-12-00673-g001.jpg

0.416931

dfb07b20ffaf415899a2066c8ad1d5f2

Amphiphilic AuNPs and amphiphilic peptides: a common affinity for the disordered domains of phase−separated lipid membranes. (a) Phase−separated SLBs containing 1,2-dioleoyl-sn-glycero-3-phosphocholine (DOPC), sphingomyelin (SM), cholesterol and ganglioside GM1 (63:31:1:5 molar ratio) imaged in liquid by atomic force microscopy (AFM) after addition of ~3 nm MUS:OT AuNPs (40 min and 15 h). After hours, large clusters of amphiphilic AuNPs (white arrows) slowly formed on the darker disordered phase and at the edges of the lighter (i.e., higher) ordered lipid domains. (b) Potential of mean force (PMF) profiles calculated for the adsorption of a single MUS:OT AuNPs on the surface of the Ld and Lo phase. The Ld phase, with a binding free energy of ~18 kJ/mol (~9 kBT), is favoured over the Lo phase (~11 kJ/mol, ~5 kBT). (c) Giant plasma membrane vesicles (GPMVs) derived from rat basophilic leukemia cells incubated at low temperature with three examples of fluorescein-labeled CPPs—i.e., MAP (model amphipathic peptide), penetratin (pAntp) and transportan 10 (TP10) (green). All these CPPs are amphipatic and contain Lys or Arg residues. Lo and Ld phases are labeled with AF594-labeled cholera toxin B subunit (CtxB, red) and AF647-labeled annexin V (AnV, pseudocolored as white), respectively. (a,b) contain images by Canepa et al. [44] reprinted with minor modifications under a CC BY-NC 3.0 license with permission from the Royal Society of Chemistry. (c) is reproduced and adapted with permission from Säälik et al. [81]—Copyright © 2011 Elsevier B.V. All rights reserved.

PMC9324301

membranes-12-00673-g002.jpg

0.440193

97bea8bef09d40d8af374c749b9ed027

Perturbation of membrane ordered–disordered phase separation upon interaction with amphiphilic AuNPs and amphiphilic peptides. (a) Topographic AFM images showing fragmentation of ordered domains induced by ~3 nm MUS:OT AuNPs on phase-separated lipid bilayers containing DOPC:SM:chol:GM1 (63:31:1:5 molar ratio). Two height profiles of the phase-separated membrane without NPs are also reported. On the right: comparison of height difference distributions (Δz) between ordered and disordered domains before and after NP/membrane interaction. (b) Fluorescence images of the same region of a POPC:1,2-dimyristoyl-sn-glycero-3-PG (DMPG) 1:1 phase-separated SLB with PG-enriched ordered domains recorded before and after exposure to the lipopetide DAP (1 μM). Two solid ordered domains (dark regions) in a liquid disordered background (bright region) containing the fluorescence lipid probe DHPE-Texas Red (TR, 1%) are shown. The channel of kynurenine (KYN)—an intrinsically fluorescent DAP residue—is used to visualize the morphology of ordered domains since DAP strongly interacts with PG lipids. Overall, the SLB/DAP interaction induces an extensive size reduction of the solid domains equal to 59%. (c) Force spectroscopy analysis and topographic AFM images of the same POPC:DMPG 1:1 SLB before and after exposure to increasing DAP concentrations (0–8 μM). Jump-through (J–T) force maps (resolution 32 × 32 pixels) are reported next to each AFM image, together with the comparison of solid and fluid domains jump-through force upon increasing concentrations of DAP. (a) contains images by Canepa et al. [44] reprinted with minor modifications under a CC BY-NC 3.0 license with permission from the Royal Society of Chemistry. (b,c) are reproduced and adapted with permission from Mescola et al. [99]—Copyright © 2020 American Chemical Society. All rights reserved.

PMC9324301

membranes-12-00673-g003.jpg

0.438654

bde5e2e432394a43833a07cd6fcc33d9

Translocation of amphiphilic AuNPs and amphiphilic peptides is favoured into lipid membranes with lower cholesterol content. (a) Left: coarse-grained structure of hydrophilic (MUS) and hydrophobic (OT) ligands and an amphiphilic MUS:OT AuNP in water (2 nm core size; water not shown). Right: simulation snapshots showing the ligand anchoring typical of these AuNPs (see Figure 1b). The NP goes from the hydrophobic contact state (top) to the anchored state (bottom) in which one MUS charged terminal is in contact with the lipid heads (transparent gray) of the distal leaflet. Cholesterol molecules—intercalated between the apolar tails of membrane phospholipids (DOPC)—are shown in tan in the membrane detail on the bottom left. (b) Anchoring free energy barriers calculated with well-tempered metadynamics simulations at different membrane cholesterol concentrations. (c) Average anchoring time (Δtanchor) and average number of anchored ligands after 1 μs (Nanchors) obtained from unbiased MD simulations as a function of membrane cholesterol content. (d) Translocation of the Arg-rich CPP nona-arginine (Arg9)—labeled with fluorescein (green)—into GPMVs derived from MDA-MB-231 (MDA GPMV) and RBL-2H3 (RBL GPMV) cells. Left images: GPMVs labeled with filipin (pseudo-colored as white) to bind membrane cholesterol and enable its visualization. Right images: GPMVs labeled with Alexa Fluor 555-conjugated cholera toxin B subunit (CtxB, red) and Alexa Fluor 647-conjugated annexin V (AnV, pseudocolored as white) to visualize, respectively, the Lo membrane domains and phosphatidylserine contained in the outer leaflet of the limiting membrane of both vesicle types. Quantification of the filipin signal shows that MDA GPMVs contain approximately 30% less membrane cholesterol than RBL GPMVs; in addition, RBL GPMVs show several large cholesterol-enriched subdomains (white arrows) that are rarer in MDA GPMVs. Overall, Arg9 translocation is significantly reduced in vesicles characterized by higher membrane cholesterol content and more cholesterol-rich membrane microdomains. *** p-value < 0.0001 and 0.0005 for filipin signal and fluo-Arg9 translocation, respectively. (a–c) contain images by Canepa et al. [45] reprinted with minor modifications—Copyright © 2021 The Authors, published by American Chemical Society. (d) is reproduced and adapted with permission from Lorents et al. [111]—Copyright © 2018 American Chemical Society.

PMC9324301

membranes-12-00673-g004.jpg

0.419943

b1beba99207f4a14940cd84e097850af

The tendency for membrane aggregation is shared by amphiphilic AuNPs and amphiphilic peptides. (a) Top, sketch of two curvature inducing membrane inclusions; due to the elastic energy of the membrane, an effective interacting potential can arise and, depending on the system, the interaction can be attractive and thus lead to aggregation. Bottom, system in which the inclusions suppress the natural fluctuations of the membrane; aggregation can minimize this region. (b) Aggregation induced by lipid depletion. (c) Aggregation induced by capillary forces. In configuration (1), there are two regions of modified lipid density around the inclusion; dimerization allows to minimize their total area, as shown in configuration (2). (d) Ordered aggregate of MUS:OT NPs adsorbed on a DOPC membrane. The snapshot is taken from unbiased MD simulations (Martini CG). Nanoparticles are represented with yellow beads (Au), pink beads (S), cyan (MUS ligands) and blue (OT ligands); membrane lipids are represented with red beads. (e) Dimer of adsorbed NPs on DOPC membrane from unbiased MD simulations (Martini CG). The extended ligand configuration can be observed. Representation as in (d). (f) Cryo-EM image of MUS:OT aggregation on the surface of a DOPC liposome. The NP-NP is compatible with the extended ligand configuration. (g) Ordered aggregate of MUS:OT NPs embedded in a model neuronal plasma membrane. The snapshot is taken from unbiased MD simulations (Martini CG). The NP are represented with a yellow core, blue OT ligands and cyan MUS ligands. The membrane is represented with red DliPC lipids, light pink sphingomyelin, yellow ganglioside and grey cholesterol. (h) Dimer of MUS:OT NPs embedded in a model neuronal plasma membrane. The deformation of the ligand shell and the presence of the stabilizing layer of ions (red beads) can be observed. The membrane headgroups are shown as semi-transparent surface, lipid tails are not shown for clarity. (i) Supramolecular lattice formed by M1 bilayer-embedded MUS:OT NPS, imaged by AFM. The digital zoom of the area with blue contour shows the lattice order at higher magnification. (a–c) adapted with permission from Johannes et al. [152] Copyright © 2022 Elsevier B.V. All rights reserved. (d–f) adapted from Lavagna et al. [173] with permission from the Royal Society of Chemistry. (g–i) adapted from Canepa et al. [44] under a CC BY-NC 3.0 license with permission from the Royal Society of Chemistry.

PMC9324301

membranes-12-00673-g005.jpg

0.475652

8d7c89a4943642c0a1fa50b5da1264a1

Pathways affected by COPD status [16].

PMC9324381

metabolites-12-00621-g001.jpg

0.433705

09ab333216e84dc3ab13883239497c8f

Inclusion/Exclusion Flowchart.

PMC9324381

metabolites-12-00621-g002.jpg

0.411665

a4b1bc8bae5d499e80ceb3ba9321ea8c

Cumulative incidence function for composite outcome (death, stroke, systemic embolism, gastrointestinal, or intra-cranial haemorrhage) by frailty category and time-varying anticoagulation status (with 95% confidence intervals).

PMC9326851

euac022f1.jpg

0.418244

fa5b85c5311e44e9b86e47a8f2e7c33d

Cumulative incidence function for all-cause death by frailty category and time-varying anticoagulation status (with 95% confidence intervals).

PMC9326851

euac022f2.jpg

0.405207

6cc4e99943bf4616a285ddce2331837a

Cumulative incidence function for stroke by frailty category and anticoagulation status (with 95% confidence intervals).

PMC9326851

euac022f3.jpg

0.392181

5b4b4b995780421c95b9daad8d595252

Cumulative incidence function for severe bleeding by frailty category and anticoagulation status (with 95% confidence intervals).

PMC9326851

euac022f4.jpg

0.448668

6d4ff7e3d3424474b12e523b9ea0b96f

Cumulative incidence function for transient ischaemic attack by frailty category and anticoagulation status.

PMC9326851

euac022f5.jpg

0.42629

04d2c3e2b4674fbabc9cba06d63346bc

High blood lead (Pb) level (BLL) locations identified with Getis-Ord Gi* geospatial cluster analysis for exceedance rate of EBLLs (≥5μg/dL) in census tracts and reference locations (2014–2016; children 0 to <6 years of age; 2,401 total census tracts evaluated). See also Table 2. Note: EBLL, elevated blood lead level.

PMC9327739

ehp9705_f1.jpg

0.451622

74294d9111d347499e060a0787d466b7

Getis-Ord Gi* geospatial cluster analysis for exceedance rate of elevated blood lead (Pb) levels (≥5μg/dL) in census tracts by years (children 0 to <6 years of age; number of census tracts evaluated: (A) 2006 to 2013, 2,400; (B) 2011 to 2013, 2,405; (C) 2014 to 2016, 2,401. See also Table 3.

PMC9327739

ehp9705_f2.jpg

0.471004

2373dcfeb39e48d6962e1e5bc8213336

Getis-Ord Gi* geospatial cluster analysis for exceedance rate of elevated blood lead (Pb) levels [(A) ≥5μg/dL; (B) ≥10μg/dL] in census tracts by previous Centers for Disease Control and Prevention (CDC) reference values (2014–2016; children 0 to <6 years of age; 2,401 census tracts evaluated). See also Table 3.

PMC9327739

ehp9705_f3.jpg

0.430574

8b437f3ee02f44498753ca7562dabe31

Changes over time for exceedance rate of %EBLLs by census tracts—lower peninsula of Michigan (number of census tracts evaluated: (A) 2006–2007, 2,244; (B) 2008–2010, 2,383; (C) 2011–2013, 2,405; (D) 2014–2016, 2,401). See also Table 1 and Table S1. Note: %EBLLs, percentage elevated blood lead levels.

PMC9327739

ehp9705_f4.jpg

0.470399

d9e19f9932ce4ff99e817bdd7281fd37

Getis-Ord Gi* geospatial cluster analysis for (A) EJSCREEN 2017 Pb Paint EJ Index (2,752 census tracts evaluated), (B) Schultz et al. regression model13 approach BLL Data (2,774 census tracts evaluated), and (C) HUD Deteriorated Paint Index (2,741 census tracts evaluated) displayed side by side with (D) the Getis-Ord Gi* cluster analysis for exceedance rate of EBLLs (2,401 census tracts evaluated; ≥5μg/dL; 2014–2016; children 0 to <6 years of age). See also Table 3. Note: BLL, blood lead level; EBLL, elevated blood lead level; EJ, environmental justice; EPA, Environmental Protection Agency; HUD, U.S. Department of Housing and Urban Development; Kappa, Cohen’s Kappa agreement statistic (0.41–0.6, moderate; 0.61–0.8, substantial; 0.81–0.99, near perfect agreement); Pb, lead.

PMC9327739

ehp9705_f5.jpg

0.449596

72bfcd8003d6491f9685eeaaad1a966d

The process of content analysis

PMC9327824

HEX-25--g001.jpg

0.49116

783c582b138d416190903a48266b1fff

Timeline of NCCN APP Workgroup and surveys.

PMC9328455

jadpro-13-507-g001.jpg

0.412938

a03306d732d843588083c8b7240c3681

Survey question on RVUs as an indicator of APP productivity (N = 489).

PMC9328455

jadpro-13-507-g002.jpg