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The authors present an interesting case study integrating geophysical, geological and hydrochemical data to map potential pathways for water and gases across complex sedimentary formations in the Northern Territories, in Australia.
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- Line 303: “We reviewed the seismic data in detail at the intersections with north-northwest trending lineaments observed on the magnetic dataset (Figure 4).” No such lineaments are shown in Figure 4.
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geosciences12010037_makarova
0
The depth maps provided in Figure 5 should be supplemented by isochore maps for the rock volumes between pairs of mapped reflectors.
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To my understanding this should correspond to Figure 2 a, which shows substantially more than 130 lineaments.
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geosciences12010037_makarova
0
These often highlight tectonic accommodation space creation better than maps.
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Recent fault activity was identified using a combination of sub-surface imaging and comparison of fault orientations and surface drainage networks.
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geosciences12010037_makarova
0
One of the key issues in the Beetaloo Sub-basin, the risk of groundwater contamination from fracking-based production of the unconventional plays.
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All comment avec been considerate: figures 1,4,7,8, 9 have been
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geosciences12010037_makarova
0
); PossibleFluidEscapeFeatures to Possible fluid-escape features etc.
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We added: “We define as recent tectonic activity the events that are recorded at the surface and can alter the surface with active tectonic features, such as modifications of the drainage system” • Discussion: One general comment, could the use of other tracers (radon, of stable isotopes of water) yield also insight on the origin of the fluids sampled in this study?
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geosciences12010037_makarova
0
According to the new caption shows the unfiltered surface drainage network.
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Consider removing labels not referred to in the text.
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geosciences12010037_makarova
0
Overall, the implications of this study are important not only for industrial purposes (reservoir exploration and operation) but also for our basic understanding of subsurface flow processes over geological timescales.
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Consider using higher contrast colours or thicker lines to highlight different faults on the map.
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geosciences12010037_makarova
0
Development of the petroleum resources in this region is controversial and faces opposition from Traditional Owners as well as climate scientists, and environmentalists.
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Conclusions appear supported by the observations made in this study, but presentation of results needs to be improved.
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geosciences12010037_makarova
0
However, there are still a couple of minor issues.
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Helium content of the aquifer systems was sampled and measured.
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geosciences12010037_makarova
0
Methods are sound and clearly described, There are some shortcomings in documentation and presentation of results, which weaken the link between observations and conclusion.
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Magnetic data and 2D seismic reflection profiles were used to image structural features, and fluid/gas leakage pathways.
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geosciences12010037_makarova
0
These often highlight tectonic accommodation space creation better than maps.
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We separated the legends from figures 1A and 1B as to add clarity.
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geosciences12010037_makarova
0
Not possible to differentiate “ntspr_2M_gw” (whatever that is) and “PossibleFluidEscapeStructures” (same colour).
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Helium isotope data do not provide an unequivocal answer to whether the gas originates from the unconventional plays or a deeper source.
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geosciences12010037_makarova
0
This figure could be improved by adding shaded satellite image to help the reader compare the mapped drainage systems (which were automatically mapped) and fault traces/lineaments with the real features observed.
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If these items are present, please use a more contrasting signatures to make it more visible.
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geosciences12010037_makarova
0
We tried to improve the figures at best- attached is a detailed reply to reviews.
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It doesn’t read well otherwise.done • Figure 7: What is special about the locations highlighted by the markers (crosses on Figure 7B and square markers on Figure 7C).
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geosciences12010037_makarova
0
Methods are sound and clearly described, There are some shortcomings in documentation and presentation of results, which weaken the link between observations and conclusion.
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The approach used by the authors is innovative and allows to elaborate several hypotheses regarding the occurrence and movement of both paleo and more recent fluids.
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geosciences12010037_makarova
0
Alternatively the position of Figure 6 A, B and C should be indicated in Figure 4 as well as Figure 3. done Figure 5.
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Review summary Scientific aims clearly stated.
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geosciences12010037_makarova
0
We followed those advises and updated the document consequently.
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One of the key issues in the Beetaloo Sub-basin, the risk of groundwater contamination from fracking-based production of the unconventional plays.
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geosciences12010037_makarova
0
All comment avec been considerate: figures 1,4,7,8, 9 have been
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Figure 2 - Poor quality figure (low resolution) in the version available to the reviewer.
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geosciences12010037_makarova
0
The present paper is thus part of an ongoing debate and the findings presented here are likely to influence discussions and decisions by stakeholders.
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• Lines 271-272: Why is the signal quality so variable, and how were the categories (excellent, average and poor) defined?
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geosciences12010037_makarova
0
The replies to detailed comments are in purples, directly below each comment Frery et al.
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Fault and surface drainage network mapping (tectonic geomorphology).
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geosciences12010037_makarova
0
Outline size modified and homogenised with other figures 4.
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With respect to the helium measurements, no detailed documentation of where these samples were collected is provided beyond referring to Geological and Bioregional Assessment Program (2021a) Fact sheet 12, and Geological and Bioregional Assessment Program (2021b) Regional tracer results from the Cambrian Limestone Aquifer.
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geosciences12010037_makarova
0
what differentiates “FAULT” from a “BaseCambrianSeismic fault”?).
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Why are these not classified in terms of signal quality?
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geosciences12010037_makarova
0
It is not clear if the surface drainage network line dataset is based on the “unfiltered” or “simplified” data shown in Figure 2.
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Thank you, the details comments on those figures have been followed and new figures are provided Helium measurements from groundwater samples.
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geosciences12010037_makarova
0
The study concludes that there the shallow aquifers are connected to deep-seated gas source along faults, some of which show evidence of recent movement.
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• Figure 6: Put the title of each subfigure above the plot.
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geosciences12010037_makarova
0
Reference is made to “…prominent […] magnetic lineaments which can be caused by faulting”, and “…several circular low magnetization anomalies, approximately two kilometers in diameter, aligned with northwest trending lineaments”.
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done -Many well-name labels are hard difficult to read/illegible.
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geosciences12010037_makarova
0
done -Many well-name labels are hard difficult to read/illegible.
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If the latter applies, make sure to provide a scale.
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geosciences12010037_makarova
0
It would be nice to include a map with these features and lineaments interpreted from the aeromagnetic datasets.
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Reviewer 2: Summary of content: The study investigates potential connections between unconventional petroleum plays and water assets in the Beetaloo Sub-basin in the Northern Territory, Australia.
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geosciences12010037_makarova
0
done Figure 4 -Poor quality figure (low resolution) in the version available to the reviewer.
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The depth maps provided in Figure 5 should be supplemented by isochore maps for the rock volumes between pairs of mapped reflectors.
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geosciences12010037_makarova
0
done Figure 1B -Consider adding a small inset table or schematic to showing the Cambrian Limestone Aquifer stratigraphy in the Georgina, Daly and Wiso basin.
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the seismic interpretation of figure 7G (cross) that were misleading the reader and add the interpretation of the fluid leakage features on the images • Figure 9: Remove ‘s’ from <10 yearss.
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geosciences12010037_makarova
0
Depth conversion using check-shot velocities from 26 wells.
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done Figure 1B -Consider adding a small inset table or schematic to showing the Cambrian Limestone Aquifer stratigraphy in the Georgina, Daly and Wiso basin.
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geosciences12010037_makarova
0
Helium content of the aquifer systems was sampled and measured.
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what differentiates “FAULT” from a “BaseCambrianSeismic fault”?).
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geosciences12010037_makarova
0
The results are of interest to both surgeons and haematologists/haemostaseologists.
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For example, the section begins with introducing inherited thrombophilias and VTE risk in these conditions, then proceeds to surgery, then back again to thrombophilia where it repeats itself and even states other numbers for prevalences and VTE risks than previously stated.
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healthcare10030476_makarova
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Please explain, or consider omitting this statement altogether.
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Answer to the Reviewer Point 1: The observation of the reviewer has been accepted and the table 1 was modified accordingly.
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healthcare10030476_makarova
0
The results are of interest to both surgeons and haematologists/haemostaseologists.
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2-3% of what for the 20210 polymorphism and 3-8% of what for FV Leiden?
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healthcare10030476_makarova
0
Please explain, or consider omitting this statement altogether.
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Point 4: In the Materials and methods section, several questions arise: A)
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healthcare10030476_makarova
0
Answer to the Reviewer point 7: The observation of the reviewer has been accepted and the manuscript was modified accordingly.
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However, I have several concerns which must be adequately addressed before the manuscript is suitable for publication.
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healthcare10030476_makarova
0
Answer to the Reviewer Point 1: The observation of the reviewer has been accepted and the table 1 was modified accordingly.
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We also declare that we have no conflict of interest in connection with this paper.
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healthcare10030476_perova
0
Author Response Reviewer(s)' Comments to Author: Reviewer 2: (x) Extensive editing of English language and style required Answer to the Reviewer: The new manuscript has been evaluated by an expert of English language.
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D) Page 3, lines 140-141: “We collected data for all groups of patients in terms of drain permanence, thromboembolic events, and hematoma formation.” How did you define and verify a thromboembolic event?
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healthcare10030476_perova
0
Answer to the Reviewer Point 2: The new manuscript has been evaluated by an expert of English language.
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Point 5: The Discussion section could benefit from a language revision, especially in the latter part, to Answer to the Reviewer point 5: The observation of the reviewer has been accepted and the manuscript was modified accordingly.
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healthcare10030476_perova
0
2-3% of what for the 20210 polymorphism and 3-8% of what for FV Leiden?
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The authors report experience from their surgical center with performing abdominoplasty in women with "thrombophilia" which is not clearly defined.
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healthcare10030476_perova
0
We thank the Editor and the Reviewers for helping us to improve our paper.
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Point 2: Also, another round of language editing may be suitable.
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healthcare10030476_perova
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I think, the manuscript benefits from a minor language editing.
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Also, the research questions lack describing the study setting.
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healthcare10040723_perova
0
I suggest to remove the phrase ‘so called,’ that has been used in the conclusion section or any other section.
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For example, the first sentence should describe the general aim or the overall objective of the study in a more general term.
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healthcare10040723_perova
0
However, the first sentence did not appear to serve this purpose.
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Page 3, lines 117-118, the statement has two issues.
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healthcare10040723_perova
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I suggest to re-write the aims and study questions more clearly.
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I would ask, why did you use convenience sampling?
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healthcare10040723_perova
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However, the first sentence did not appear to serve this purpose.
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It needs the careful wording of the study participants.
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healthcare10040723_perova
0
Page 4, table 1, the caption and the table content do not match.
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‘The items were then tested through a pilot study consisting of a 117 group of 4 pre- and clinical students, mediated by AG and JE’.
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healthcare10040723_perova
0
I suggest to re-write the aims and study questions more clearly.
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I am suggesting this because the logic of quantitative sampling and qualitative sampling are different.
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healthcare10040723_perova
0
It needs the careful wording of the study participants.
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Also, the research questions lack describing the study setting.
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healthcare10040723_perova
0
However, the first sentence did not appear to serve this purpose.
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Page 13, lines 409-410, in the conclusion section, the statement which says, ‘This study also found significant differences between those groups indicating differences in subgroups of students from the quantitative survey.’ This is a confusing statement.
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healthcare10040723_perova
0
I am suggesting this because the logic of quantitative sampling and qualitative sampling are different.
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I suggest the authors to replace this with appropriate phrase.
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healthcare10040723_perova
0
I think, the manuscript benefits from a minor language editing.
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Also, the research questions are not clearly stated.
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healthcare10040723_perova
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Around 38% of the students felt ill-prepared and could not answer AI-related questions because digitization in medicine and AI are not a formal part of the medical curriculum.
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1 Review comments Title: Medical students' perceptions towards digitization and artificial intelligence: A mixed methods study Thank you for the opportunity to review this manuscript.
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healthcare10040723_perova
0
Page 13, lines 409-410, in the conclusion section, the statement which says, ‘This study also found significant differences between those groups indicating differences in subgroups of students from the quantitative survey.’ This is a confusing statement.
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Page 3, lines 117-118, the statement has two issues.
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healthcare10040723_perova
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It needs the careful wording of the study participants.
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Also, the research questions lack describing the study setting.
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healthcare10040723_perova
0
In the abstract, lines 23-27, I suggest to use the following expression or something like that.
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Page 12, line 389, the statement which says, ‘The non-probabilistic sampling makes a generalization of the answers difficult,’ is confusing.
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healthcare10040723_perova
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Page 4, table 1, the caption and the table content do not match.
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I hope these suggestions are of use to the authors.
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healthcare10040723_perova
0
Dear Authors, Here attached, please find my comments for the improvement of your paper.
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The paper is well written in terms of clarity of idea and the authors use of mixed-methods design was interesting.
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healthcare10040723_perova
0
Page 13, lines 409-410, in the conclusion section, the statement which says, ‘This study also found significant differences between those groups indicating differences in subgroups of students from the quantitative survey.’ This is a confusing statement.
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The aim of the study as reported in the manuscript was to explore attitudes of 2020 medical students’ generation towards various aspects of eHealth technologies with the focus on AI using an exploratory sequential mixed method analysis.
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healthcare10040723_perova
0
In the abstract, lines 23-27, I suggest to use the following expression or something like that.
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I suggest to remove the phrase ‘so called,’ that has been used in the conclusion section or any other section.
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healthcare10040723_perova
0
Response 10: Special thanks to you for your good suggestion.
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Response to Reviewer 1 Comments Point 1: "back of tobacco" is not clear be more specific.
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horticulturae8050412_makarova
0
In a previous study (Ding, 2021), the LlWRKY39 promoter was fused to a pGreenII-0800-LUC vector using a recombinant ligase (Vazyme, Nanjing, China).
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Point 7: Line 311: plant leaves instead of plants leaves.
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horticulturae8050412_makarova
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This sentence has been changed to “To better understand why OfSPL11 transgenic lines exhibit enhanced abiotic stress tolerance, we analyzed the expression of known abiotic stress response genes in 3-week-old wild-type and transgenic Arabidopsis.” Point 4: In lines 271-272 and 395-396, how do you explain that salt induced promoter trans-activation?
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Response 4: We feel sorry for the unclear statement.
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horticulturae8050412_makarova
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In this study, the experimental operations, reagents and plant materials were were the same as the previous study, except for the different treatment conditions.
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Point 11: In line 100 replace "ligated" with was ligated and quote a reference for pORER4-GFP vector or mention brand if it is commercial.
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horticulturae8050412_makarova
0
In this study, the experimental operations, reagents and plant materials were were the same as the previous study, except for the different treatment conditions.
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The constructed vector and an empty vector (control) were transformed into A. tumefaciens strain GV3101 (pSoup), which was used to transform N. benthamiana leaves.
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horticulturae8050412_makarova
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Point 4: Line 267-268: Please, check this sentence.
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Therefore, we suggest that salt stress can induce the activity of the OfSPL11 promoter.
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horticulturae8050412_makarova
0
Point 22: In line 261 replace "tobaccos" with tobacco plants.
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Point 9: Line 360: remove the space after "assay".
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horticulturae8050412_makarova
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Point 14: In line 118 define "symbiotic medium".
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Point 11: Line 392: I suggest to change the sentence in the following way".. to drive the expression of target genes.." Response 11: Thank you for your comment.
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horticulturae8050412_makarova
0
Results clearly describe the protein localization as well as its role in stress response.
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In a previous study (Ding, 2021), the LlWRKY39 promoter was fused to a pGreenII-0800-LUC vector using a recombinant ligase (Vazyme, Nanjing, China).
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horticulturae8050412_makarova
0
Point 22: In line 261 replace "tobaccos" with tobacco plants.
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The LUC signal increased profoundly after subjection to HS (37℃), although under normal conditions (room temperature), the LUC signal was very low, which implied that HS activated the promoter activity of LlWRKY39.
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horticulturae8050412_makarova
0
The constructed vector and an empty vector (control) were transformed into A. tumefaciens strain GV3101 (pSoup), which was used to transform N. benthamiana leaves.
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Results clearly describe the protein localization as well as its role in stress response.
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horticulturae8050412_makarova
0
Point 23: In line 262 replace "were treatment by" with were treated with.
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; Zhang, D.H.; Teng, N.J. LlWRKY39 is involved in thermotolerance by activating LlMBF1c and interacting with LlCaM3 in lily (Lilium longiflflorum).
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horticulturae8050412_makarova
0
Response to Reviewer 2 Comments Point 1: Why the authors do not consider to use aminoacidic sequence to compute a protein model (e.g., by using gromacs)?
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Point 12: In line 113 replace "psoup" with pSOUP and "tobaccos" with tobacco plants.
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horticulturae8050412_makarova
0
In this study, the experimental operations, reagents and plant materials were were the same as the previous study, except for the different treatment conditions.
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Point 14: In line 118 define "symbiotic medium".
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horticulturae8050412_makarova
0
Major corrections The manuscript by Zhu et al., describes the cloning of OfSPL1 transcriptional activator from Osmanthus fragans, its molecular characterization, subcellular localization, gene-expression, trans-activation in tobacco cells and two-hybrid system, and overexpression in Arabidopsis, displaying and enhanced salt-tolerance phenotype and protection from oxidative damage.
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Point 14: In line 118 define "symbiotic medium".
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horticulturae8050412_makarova
0
In this view, I've only some few questions for the authors.
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tumefaciens suspensions containing the fusion vector OfSPL11pro: LUC was transiently transformed into O. fragrans callus using vacuum infiltration, and then the O. fragrans callus were cultured in a symbiotic medium containing 0 mM and 200 mM NaCl.” Point 5:
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horticulturae8050412_makarova
0
Response 9: Thank for your kindly suggestion.
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Results clearly describe the protein localization as well as its role in stress response.
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horticulturae8050412_makarova
0
Point 7: In line 65 replace "And" with In addition.
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Point 25: In line 292 "The" must be lowercase.
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horticulturae8050412_makarova
0
Major corrections The manuscript by Zhu et al., describes the cloning of OfSPL1 transcriptional activator from Osmanthus fragans, its molecular characterization, subcellular localization, gene-expression, trans-activation in tobacco cells and two-hybrid system, and overexpression in Arabidopsis, displaying and enhanced salt-tolerance phenotype and protection from oxidative damage.
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Point 11: Line 392: I suggest to change the sentence in the following way".. to drive the expression of target genes.." Response 11: Thank you for your comment.
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horticulturae8050412_makarova
0
Response 5: Thank for your kindly suggestion.
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The paper by Zhu and coworkers deal with the functional deeeping on te role of gene OfSPL11 from Osmanthus fragrant.
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horticulturae8050412_makarova
0
In addition, heterologous overexpression promotes induction of several genes involved in tolerance to abiotic stress.
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Point 7: Line 311: plant leaves instead of plants leaves.
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horticulturae8050412_makarova
0
Point 22: In line 261 replace "tobaccos" with tobacco plants.
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Results clearly describe the protein localization as well as its role in stress response.
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horticulturae8050412_makarova
0
Point 11: Line 392: I suggest to change the sentence in the following way".. to drive the expression of target genes.." Response 11: Thank you for your comment.
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In this view, I've only some few questions for the authors.
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horticulturae8050412_makarova
0
Do the authors have performed an efficiency test before using it?
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Point 7: In line 65 replace "And" with In addition.
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horticulturae8050412_makarova
0
The LUC signal increased profoundly after subjection to HS (37℃), although under normal conditions (room temperature), the LUC signal was very low, which implied that HS activated the promoter activity of LlWRKY39.
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We have revised the manuscript according to your comments (Line 274-277).
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horticulturae8050412_makarova
0
Results clearly describe the protein localization as well as its role in stress response.
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We have revised the manuscript according to your comments (Line 274-277).
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horticulturae8050412_makarova
0
Response to Reviewer 1 Comments Point 1: "back of tobacco" is not clear be more specific.
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Results clearly describe the protein localization as well as its role in stress response.
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horticulturae8050412_makarova
0
Response to Reviewer 1 Comments Point 1: "back of tobacco" is not clear be more specific.
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Point 25: In line 292 "The" must be lowercase.
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horticulturae8050412_makarova
0
Point 17: In line 131 delete the period after experiments.
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Line 315: maybe a semicolon after "salt stress" is appropriate
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horticulturae8050412_makarova
0
In addition, heterologous overexpression promotes induction of several genes involved in tolerance to abiotic stress.
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Line 315: maybe a semicolon after "salt stress" is appropriate
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horticulturae8050412_makarova
0
Therefore, we suggest that salt stress can induce the activity of the OfSPL11 promoter.
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It ends with "then" Response 4: Thank you for your comment.
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horticulturae8050412_makarova
0
Point 7: Line 311: plant leaves instead of plants leaves.
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Response 3: Special thanks to you for your good suggestion.
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horticulturae8050412_makarova
0
Point 4: Line 267-268: Please, check this sentence.
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Point 22: In line 261 replace "tobaccos" with tobacco plants.
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horticulturae8050412_makarova
0
Point 12: In line 113 replace "psoup" with pSOUP and "tobaccos" with tobacco plants.
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A deeeping on protein an nucleotide structures was also reported.
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horticulturae8050412_makarova
0
We have revised the manuscript according to your comments (Line 274-277).
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Point 12: In line 113 replace "psoup" with pSOUP and "tobaccos" with tobacco plants.
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horticulturae8050412_makarova
0
Point 12: In line 113 replace "psoup" with pSOUP and "tobaccos" with tobacco plants.
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Line 315: maybe a semicolon after "salt stress" is appropriate
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horticulturae8050412_makarova
0
Response to Reviewer 2 Comments Point 1: Why the authors do not consider to use aminoacidic sequence to compute a protein model (e.g., by using gromacs)?
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Point 7: Line 311: plant leaves instead of plants leaves.
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horticulturae8050412_makarova
0
; Bustin, S.A. Validation of housekeeping genes for normalizing RNA expression in real-time PCR.
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Point 6: In line 63 replace "trancroptoma" with transcriptome.
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horticulturae8050412_perova
0
Point 9: Line 360: remove the space after "assay".
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We have revised the manuscript according to your comments (Line 274-277).
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horticulturae8050412_perova
0
Point 27: In line 395 replace "it revealed" with revealed.
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Point 26: In line 318 "We" must be lowercase.
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horticulturae8050412_perova
0