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; Zhang, D.H.; Teng, N.J. LlWRKY39 is involved in thermotolerance by activating LlMBF1c and interacting with LlCaM3 in lily (Lilium longiflflorum).
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Point 22: In line 261 replace "tobaccos" with tobacco plants.
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horticulturae8050412_perova
| 0 |
Furthermore, I reccomend a text revision since some errors are present in the main text.
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Point 7: Line 311: plant leaves instead of plants leaves.
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horticulturae8050412_perova
| 0 |
Point 27: In line 395 replace "it revealed" with revealed.
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Response 9: Thank for your kindly suggestion.
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horticulturae8050412_perova
| 0 |
Point 16: In line 129 replace "strains and "strain" with lines and line.
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Response 4: We feel sorry for the unclear statement.
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horticulturae8050412_perova
| 0 |
Point 24: In line 264 replace "indicate" with indicated.
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Point 7: Line 311: plant leaves instead of plants leaves.
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horticulturae8050412_perova
| 0 |
Response to Reviewer 1 Comments Point 1: "back of tobacco" is not clear be more specific.
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Point 11: Line 392: I suggest to change the sentence in the following way".. to drive the expression of target genes.." Response 11: Thank you for your comment.
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horticulturae8050412_perova
| 0 |
Point 28: In line 406 delete period after stress.
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Identification of Suitable Reference Genes for Gene Expression Normalization in the Quantitative Real-Time PCR Analysis of Sweet Osmanthus (Osmanthus fragrans Lour.).
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horticulturae8050412_perova
| 0 |
Major corrections The manuscript by Zhu et al., describes the cloning of OfSPL1 transcriptional activator from Osmanthus fragans, its molecular characterization, subcellular localization, gene-expression, trans-activation in tobacco cells and two-hybrid system, and overexpression in Arabidopsis, displaying and enhanced salt-tolerance phenotype and protection from oxidative damage.
| null | null |
Point 23: In line 262 replace "were treatment by" with were treated with.
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horticulturae8050412_perova
| 0 |
In addition, heterologous overexpression promotes induction of several genes involved in tolerance to abiotic stress.
| null | null |
Response to Reviewer 2 Comments Point 1: Why the authors do not consider to use aminoacidic sequence to compute a protein model (e.g., by using gromacs)?
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horticulturae8050412_perova
| 0 |
Point 10: Figure 9 caption: measure instead of measuring.
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Response 3: Special thanks to you for your good suggestion.
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horticulturae8050412_perova
| 0 |
Point 26: In line 318 "We" must be lowercase.
| null | null |
Point 10: Figure 9 caption: measure instead of measuring.
| null | null |
horticulturae8050412_perova
| 0 |
Point 6: In line 63 replace "trancroptoma" with transcriptome.
| null | null |
Response 3: Special thanks to you for your good suggestion.
| null | null |
horticulturae8050412_perova
| 0 |
Point 7: In line 65 replace "And" with In addition.
| null | null |
Response 7: Special thanks to you for your good suggestion.
| null | null |
horticulturae8050412_perova
| 0 |
Point 12: Line 446: "..participate in salt stress" Response 12: Thank for your kindly suggestion.
| null | null |
Point 24: In line 264 replace "indicate" with indicated.
| null | null |
horticulturae8050412_perova
| 0 |
Point 23: In line 262 replace "were treatment by" with were treated with.
| null | null |
; Zhao, K.; Wang, S.; Sikdar, A.; Noor, H.; Jeon, J.S.
| null | null |
horticulturae8050412_perova
| 0 |
Point 9: Line 360: remove the space after "assay".
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Response to Reviewer 1 Comments Point 1: "back of tobacco" is not clear be more specific.
| null | null |
horticulturae8050412_perova
| 0 |
Response to Reviewer 1 Comments Point 1: "back of tobacco" is not clear be more specific.
| null | null |
2021, 8,36. https://doi.org/10.1038/s41438-021-00473-7 Point 5: In line 38 replace "Fortunately" with However.
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horticulturae8050412_perova
| 0 |
Major corrections The manuscript by Zhu et al., describes the cloning of OfSPL1 transcriptional activator from Osmanthus fragans, its molecular characterization, subcellular localization, gene-expression, trans-activation in tobacco cells and two-hybrid system, and overexpression in Arabidopsis, displaying and enhanced salt-tolerance phenotype and protection from oxidative damage.
| null | null |
Response to Reviewer 1 Comments Point 1: "back of tobacco" is not clear be more specific.
| null | null |
horticulturae8050412_perova
| 0 |
Response to Reviewer 2 Comments Point 1: Why the authors do not consider to use aminoacidic sequence to compute a protein model (e.g., by using gromacs)?
| null | null |
Point 12: Line 446: "..participate in salt stress" Response 12: Thank for your kindly suggestion.
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horticulturae8050412_perova
| 0 |
; Zhao, K.; Wang, S.; Sikdar, A.; Noor, H.; Jeon, J.S.
| null | null |
Point 12: Line 446: "..participate in salt stress" Response 12: Thank for your kindly suggestion.
| null | null |
horticulturae8050412_perova
| 0 |
; Zhao, K.; Wang, S.; Sikdar, A.; Noor, H.; Jeon, J.S.
| null | null |
Point 8: Line 315: maybe a semicolon after "salt stress" is appropriate.
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horticulturae8050412_perova
| 0 |
In addition, heterologous overexpression promotes induction of several genes involved in tolerance to abiotic stress.
| null | null |
In addition, heterologous overexpression promotes induction of several genes involved in tolerance to abiotic stress.
| null | null |
horticulturae8050412_perova
| 0 |
Point 22: In line 261 replace "tobaccos" with tobacco plants.
| null | null |
Point 7: Line 311: plant leaves instead of plants leaves.
| null | null |
horticulturae8050412_perova
| 0 |
Point 12: In line 113 replace "psoup" with pSOUP and "tobaccos" with tobacco plants.
| null | null |
Point 14: In line 118 define "symbiotic medium".
| null | null |
horticulturae8050412_perova
| 0 |
This goal was achieved using transgenic lines to investigate the role of this gene in salt stress response.
| null | null |
Point 14: In line 118 define "symbiotic medium".
| null | null |
horticulturae8050412_perova
| 0 |
Response to Reviewer 1 Comments Point 1: "back of tobacco" is not clear be more specific.
| null | null |
Point 11: In line 100 replace "ligated" with was ligated and quote a reference for pORER4-GFP vector or mention brand if it is commercial.
| null | null |
horticulturae8050412_perova
| 0 |
Point 7: In line 65 replace "And" with In addition.
| null | null |
tumefaciens suspensions containing the fusion vector OfSPL11pro: LUC was transiently transformed into O. fragrans callus using vacuum infiltration, and then the O. fragrans callus were cultured in a symbiotic medium containing 0 mM and 200 mM NaCl.” Point 5: Figure 5 caption: activates instead of activate.
| null | null |
horticulturae8050412_perova
| 0 |
2015, 10,1-17. https://doi.org/10.1371/journal.pone.0136355 Point 3: Line 124: generat instead of generate.
| null | null |
2021, 8,36. https://doi.org/10.1038/s41438-021-00473-7 Point 5: In line 38 replace "Fortunately" with However.
| null | null |
horticulturae8050412_perova
| 0 |
In Silico and Transcription Analysis of Trehalose-6-phosphate Phosphatase Gene Family of Wheat: Trehalose Synthesis Genes Contribute to Salinity, Drought Stress and Leaf Senescence.
| null | null |
; Zhao, K.; Wang, S.; Sikdar, A.; Noor, H.; Jeon, J.S.
| null | null |
horticulturae8050412_perova
| 0 |
Point 11: Line 392: I suggest to change the sentence in the following way".. to drive the expression of target genes.." Response 11: Thank you for your comment.
| null | null |
In this view, I've only some few questions for the authors.
| null | null |
horticulturae8050412_perova
| 0 |
2021, 8,36. https://doi.org/10.1038/s41438-021-00473-7 Point 5: In line 38 replace "Fortunately" with However.
| null | null |
; Zhang, D.H.; Teng, N.J. LlWRKY39 is involved in thermotolerance by activating LlMBF1c and interacting with LlCaM3 in lily (Lilium longiflflorum).
| null | null |
horticulturae8050412_perova
| 0 |
Point 27: In line 395 replace "it revealed" with revealed.
| null | null |
The paper by Zhu and coworkers deal with the functional deeeping on te role of gene OfSPL11 from Osmanthus fragrant.
| null | null |
horticulturae8050412_perova
| 0 |
Point 28: In line 406 delete period after stress.
| null | null |
tumefaciens suspensions containing the fusion vector OfSPL11pro: LUC was transiently transformed into O. fragrans callus using vacuum infiltration, and then the O. fragrans callus were cultured in a symbiotic medium containing 0 mM and 200 mM NaCl.” Point 5: Figure 5 caption: activates instead of activate.
| null | null |
horticulturae8050412_perova
| 0 |
However, we used the data without outlier to construct the figure.
| null | null |
How much soil (kg) did you collect for each sample?
| null | null |
ijerph19127332_makarova
| 0 |
Table 1 – is it possible to list/ summarise the data for each day?
| null | null |
The results provided insights into the varying health risks from different have metals.
| null | null |
ijerph19127332_makarova
| 0 |
In addition, dried soil samples from living spaces (0.5 g) were digested with the same pre-treated and analyzed by ICP‒MS for Pb, As, Cr, Cu, Ni, Cd, and Zn.
| null | null |
Please, use element symbols not the entire name in all the manuscript.
| null | null |
ijerph19127332_makarova
| 0 |
Page 3, lines 121-124:Please, add some information: How did you collect dust samples? How much soil (kg) did you collect for each sample?
| null | null |
Application of more biotic samples that you suggested is useful for our study, however, it is hard to get other samples from children, such as blood and tissue.
| null | null |
ijerph19127332_makarova
| 0 |
Explain for better understanding Answer: For this question, our answer is following: this data is from the Technical Guidelines for Soil Pollution Risk Assessment of Construction Land in China.
| null | null |
Answer: Thanks for your advice, we have added the specific parameters and modified them as follows:” When the collection was completed, the samples were taken back to the laboratory and stored first in the refrigerator (‒20°C).
| null | null |
ijerph19127332_makarova
| 0 |
At last, we also added the heavy metals information in resident or park green areas: “In resident and park green areas, the highest concentration of heavy metals is Zn and the lowest is Cd.
| null | null |
Title- ‘in’ should be used for larger areas, can you add the study locations?
| null | null |
ijerph19127332_makarova
| 0 |
Answer: Thanks for your advice, we have added the specific parameters and modified them as follows:” When the collection was completed, the samples were taken back to the laboratory and stored first in the refrigerator (‒20°C).
| null | null |
Explain for better understandingWill like to see ethical approval for the project/research?
| null | null |
ijerph19127332_makarova
| 0 |
Title- ‘in’ should be used for larger areas, can you add the study locations?
| null | null |
Explain for better understandingWill like to see ethical approval for the project/research?
| null | null |
ijerph19127332_makarova
| 0 |
Besides, our study focuses on estimating the children’s SIRs of from e‒waste disassembly areas by collecting and analyzing selected tracer elements in matched samples of their consumed food, feces, and urine, as well as soil samples from their play areas.
| null | null |
Line 368 – what is the meaning of ‘Whatever’?
| null | null |
ijerph19127332_makarova
| 0 |
I’d suggest some modifications to improve the paper.
| null | null |
Page 3, lines 131-151: Please, add some information:How many millilitres of HNO3, H2O2, HF, HClO4 did you use for the digestion procedures (food samples, faces samples, soil and dust samples)?
| null | null |
ijerph19127332_makarova
| 0 |
But we failed to get all children’s feces and urine sample every day.
| null | null |
Thus, we cannot provide the data for each day.
| null | null |
ijerph19127332_makarova
| 0 |
S3 Frequency distribution histogram and outlier box (a) and Frequency distribution histogram and outlier box (b) of soil ingestion rate (SIR) based on Al”.
| null | null |
It has been changed to “The LRL is ‒112.4‒100.9 mg/d which is considered as the lower limit of the 90% confidence interval (CI) of the 2.5th percentile (P2.5), whereas the URL is 516.9‒730.2 mg/d, which is considered as the upper limit of the 90% CI of the 97.5th percentile (P97.5).
| null | null |
ijerph19127332_makarova
| 0 |
Lines 253 and 377: different values between SIR and figure 3.
| null | null |
Finally, the digest was analyzed by HR‒ICP‒MS.
| null | null |
ijerph19127332_makarova
| 0 |
26 Keywords: E‒waste; Heavy metal pollution; Children; Soil and dust ingestion rates; Health risk 27 assessment 28 29 1.
| null | null |
The median (minimum and maximum) of SIR values were ‒124.3 (‒278.0 to 228.2), ‒210.2 (‒490.1 to 273.8), 27.1 (0.4 to 106.0), ‒22532.8 (‒29443.8 to ‒6215.9), 23.9 (‒45.3 to 268.0), 175.3 (‒56.4 to 1040.7), 39.2 (‒36.4 to 284.0), and ‒263.2 (‒491.4 to 132.3) mg/d for Al, Ba, Ce, Mn, Sc, Ti, Y, and V.” Point 6.
| null | null |
ijerph19127332_makarova
| 0 |
The concentrations of heavy metals in park green areas were slightly higher than that in residential area, which may attribute to the difference of soil environmental quality management and control policy between these areas.
| null | null |
I would like to see a better description of the study location.
| null | null |
ijerph19127332_makarova
| 0 |
Integrated data from the resident and park green areas, the median concentrations (mg/kg) of Cr, Ni, Cu, Zn, As, Cd, and Pb were 48.8, 63.9, 128, 413, 6.30, 0.513, and 115, respectively.” Author Response File: Author Response.docx
| null | null |
- why different processing for different samples?
| null | null |
ijerph19127332_makarova
| 0 |
These findings provide important insights into the exposure risks of heavy metals in e‒ 25 waste dismantling sites and emphasize the health risk of Cr and As.
| null | null |
We have added the description of sampling site in the materials and methods.
| null | null |
ijerph19127332_makarova
| 0 |
Besides, our study focuses on estimating the children’s SIRs of from e‒waste disassembly areas by collecting and analyzing selected tracer elements in matched samples of their consumed food, feces, and urine, as well as soil samples from their play areas.
| null | null |
Please, check captions of figures S2 and 3 that are identical.
| null | null |
ijerph19127332_makarova
| 0 |
Experimental factors such as measurement error, source error, and transit time misalignment, may lead to some of these outliers.
| null | null |
And then samples were microwaved with HNO3‒H2O2.
| null | null |
ijerph19127332_makarova
| 0 |
Lines 116-118: The amounts of samples were confused.
| null | null |
Dried samples (1 g) were digested to evaporate at low temperatures of 55°C on a heating plate with 3 mL concentrated nitric acid, 3 mL hydrogen fluoride, and 1 mL perchloric acid (HNO3‒HF‒HClO4).
| null | null |
ijerph19127332_makarova
| 0 |
Application of more biotic samples that you suggested is useful for our study, however, it is hard to get other samples from children, such as blood and tissue.
| null | null |
The medians of the SIR values after removing the high values (see Figure S3(b) and Figure 1) were taken as the final SIR values for children living in the studied e‒waste dismantling site in South China.” Point 7.
| null | null |
ijerph19127332_makarova
| 0 |
I suggested the major revision before publication.
| null | null |
The title of Figure 1 has been changed to “Figure 1.
| null | null |
ijerph19127332_makarova
| 0 |
As an example, they may analyze the influences of food intake and food intake on heavy metal exposure.
| null | null |
Finally, the digest was analyzed by HR‒ICP‒MS.
| null | null |
ijerph19127332_makarova
| 0 |
In addition, children’s feces and urine belong to biological samples, which is sufficient to calculate the SIR.
| null | null |
Also describe in the methods sectionLine 368 – what is the meaning of ‘Whatever’?
| null | null |
ijerph19127332_makarova
| 0 |
Answer: Thanks for your advice, and we have added references to support this view, it is added “[30] Huang, W.L.
| null | null |
Crushed Evaporated samples were microwaved with 2 mL concentrated nitric acid and 3 mL hydrogen peroxide (HNO3‒H2O2).
| null | null |
ijerph19127332_makarova
| 0 |
At first, the soil heavy metal data has been added in the supporting information (Table S4 and S5).
| null | null |
Lines 176-178: Different units between manuscript and SM (figure S1).
| null | null |
ijerph19127332_makarova
| 0 |
The results provided insights into the varying health risks of different have metals.
| null | null |
This study estimated the soil ingestion of 66 17 children from e‒waste disassembly areas by collecting and analyzing selected tracer elements in 18 matched samples of their consumed food, feces, and urine, as well as soil samples from their play 19 areas.
| null | null |
ijerph19127332_perova
| 0 |
It has been re- 38 ported that the concentration of Cu was about three times higher than the Grade II guide- 39 line level (Soil Environmental Quality Standard, GB 15618‒1995) in an e‒waste disassem- 40 bly area in China, and the Cu concentrations of soils from dumping, burning, and acid 41 leaching sites were found to be 10, 40, and 60 higher, respectively, than the Grade II level.
| null | null |
It has been re- 38 ported that the concentration of Cu was about three times higher than the Grade II guide- 39 line level (Soil Environmental Quality Standard, GB 15618‒1995) in an e‒waste disassem- 40 bly area in China, and the Cu concentrations of soils from dumping, burning, and acid 41 leaching sites were found to be 10, 40, and 60 higher, respectively, than the Grade II level.
| null | null |
ijerph19127332_perova
| 0 |
I suggested the major revision before publication.
| null | null |
We have examined the tracer elements in the food.
| null | null |
ijerph19127332_perova
| 0 |
The conditions of microwave digestion are the same as mentioned above.
| null | null |
Title: Estimation of Children’s Soil and Dust Ingestion Rates and Health Risk in E Waste Dismantling Area Authors: Yan Yang * , Mengdi Zhang , Haojia Chen , Zenghua Qi , Chengcheng Liu , Qiang Chen , Tao Long Revisions Need:Title- ‘in’ should be used for larger areas, can you add the study locations?
| null | null |
ijerph19127332_perova
| 0 |
Better explanation needed - why were the feces samples not microwaved?
| null | null |
Temperature of the heating plate for faces sample digestion.
| null | null |
ijerph19127332_perova
| 0 |
In addition, children’s feces and urine belong to biological samples, which is sufficient to calculate the SIR.
| null | null |
Please, check captions of figures S2 and 3 that are identical.
| null | null |
ijerph19127332_perova
| 0 |
In addition, dried soil samples from living spaces (0.5 g) were digested with the same pre-treated and analyzed by ICP‒MS for Pb, As, Cr, Cu, Ni, Cd, and Zn.
| null | null |
The digestion process is repeated until the sample becomes sticky.
| null | null |
ijerph19127332_perova
| 0 |
Although there were 66 children involved in this study, 66 food samples, 62 feces and 64 urine samples were collected.
| null | null |
The conditions of microwave digestion are the same as mentioned above.
| null | null |
ijerph19127332_perova
| 0 |
Lines 209 and 214: check the values of Al and Sc, because they were different among discussion, Table 1 and figure 1.
| null | null |
Finally, the digest was analyzed by HR‒ICP‒MS.
| null | null |
ijerph19127332_perova
| 0 |
The digested product was diluted to 30 ml with ultrapure water and then stored at 4°C.
| null | null |
And then samples were microwaved with HNO3‒H2O2.
| null | null |
ijerph19127332_perova
| 0 |
Point 1、This study estimated the heavy metal exposure to children through soil and dust Ingestion in electronic waste (e-waste) dismantling areas.
| null | null |
Crushed Evaporated samples were microwaved with 2 mL concentrated nitric acid and 3 mL hydrogen peroxide (HNO3‒H2O2).
| null | null |
ijerph19127332_perova
| 0 |
At present, the related content in manuscript has been replaced to: “The SIR values based on the measured tracer element concentrations of food, feces, urine, soil and dust are presented in Table 1 and Figure 1.
| null | null |
Dried soil and dust samples (0.5 g) were digested as same as feces and food samples on a heating plate by HNO3‒HF‒HClO4.
| null | null |
ijerph19127332_perova
| 0 |
And then samples were microwaved with HNO3‒H2O2.
| null | null |
Sample Preparation and Instrumental Analysis -
| null | null |
ijerph19127332_perova
| 0 |
The supernatant was extracted and analyzed by High Resolution Inductively Coupled Plasma Mass Spectrometry (HR‒ICP‒MS) to determine Mn, Al, Ba, Ti, Ce, V, Sc, and Y.
| null | null |
Human Body Burden of Heavy Metals and Health Consequences of Pb Exposure in Guiyu, an E-Waste Recycling Town in China.
| null | null |
ijerph19127332_perova
| 0 |
At present, the related content in manuscript has been replaced to: “The SIR values based on the measured tracer element concentrations of food, feces, urine, soil and dust are presented in Table 1 and Figure 1.
| null | null |
The authors should provide detailed data about the concentration of heavy metals in soil and biotic samples.
| null | null |
ijerph19127332_perova
| 0 |
Line 342- Describe with references the values for carcinogenic risk and non-carcinogenic risks in the methos sections before mentioning it in the discussion section.
| null | null |
Lines 176-178: Different units between manuscript and SM (figure S1).
| null | null |
ijerph19127332_perova
| 0 |
The supernatant was extracted and analyzed by High Resolution Inductively Coupled Plasma Mass Spectrometry (HR‒ICP‒MS, Nu Attom, England) to determine Mn, Al, Ba, Ti, Ce, V, Sc, and Y. Urine samples (15 mL) were placed in a digestion tube and digested by microwaving with 2 mL of H2O2 and 3 mL of concentrated HNO3.
| null | null |
I am satisfied with the revision made on the manuscript and would like to recommend the manuscript for publication.
| null | null |
ijerph19127332_perova
| 0 |
Also describe in the methods section Answer: Thank you for your proposal.
| null | null |
Crushed Evaporated samples were microwaved with 2 mL concentrated nitric acid and 3 mL hydrogen peroxide (HNO3‒H2O2).
| null | null |
ijerph19127332_perova
| 0 |
The conditions of microwave digestion are the same as mentioned above.
| null | null |
Page 3, lines 99-100: please, add references for the following information South China's economic conditions are better than in North China, but the environmental pollution is worse.
| null | null |
ijerph19127332_perova
| 0 |
Finally, the digest was analyzed by HR‒ICP‒MS.
| null | null |
For this problem, we conducted a questionnaire survey when collecting samples before the experiment, and all the guardians of the sample providers knew and agreed to the experiment.
| null | null |
ijerph19127332_perova
| 0 |
please rephrase Answer: Thanks for your advice, for the first question, this part of the ambiguous expression has been modified to “schools should keep desks and teaching AIDs clean and tidy, and urge children to clean up after outdoor activities” Point 11.
| null | null |
S3 Frequency distribution histogram and outlier box (a) and Frequency distribution histogram and outlier box (b) of soil ingestion rate (SIR) based on Al”.
| null | null |
ijerph19127332_perova
| 0 |
Crushed Evaporated samples were microwaved with 2 mL concentrated nitric acid and 3 mL hydrogen peroxide (HNO3‒H2O2).
| null | null |
Better explanation needed Answer: Thanks for your suggestion, we have sorted out the method part again, and now the modification is as follows: “Feces samples and food samples were pretreated in the same way.
| null | null |
ijerph19127332_perova
| 0 |
The supernatant was analyzed for Al, Ba, Mn, Ti, and V by Inductively Coupled Plasma Optical Emission Spectroscopy (ICP‒OES, Spectra Arcos SOP, German) which is a method for atomic emission spectroscopy analysis using a light source that generates plasma discharge through high-frequency inductive coupling and by ICP‒MS for Ce, Sc, and Y.
| null | null |
The supernatant was extracted and analyzed by High Resolution Inductively Coupled Plasma Mass Spectrometry (HR‒ICP‒MS) to determine Mn, Al, Ba, Ti, Ce, V, Sc, and Y.
| null | null |
ijerph19127332_perova
| 0 |
And then samples were microwaved with HNO3‒H2O2.
| null | null |
Page 3, lines 116 and 120: Please, add technical details about: lyophilization of food samples.
| null | null |
ijerph19127332_perova
| 0 |
S4 Frequency distribution histogram and outlier box of soil ingestion rate (SIR) based on tracer Al(a), Ba(b), Ce(c), Mn(d), Sc(e), Ti(f), V(g), and Y(h) separately”.
| null | null |
The digested product was diluted to 30 ml with ultrapure water and then stored at 4°C.
| null | null |
ijerph19127332_perova
| 0 |
Author Response Please see the attachment Author Response File: Author Response.pdf
| null | null |
1 Response to Reviewer 2 Comments Point 1: 2.
| null | null |
jcm11051247_makarova
| 0 |
1 Response to Reviewer 2 Comments Point 1: 2.
| null | null |
Timing of TOC administration according to the oxygen flow rates As mentioned above, the cutoff value is O2 13L/min, but the oxygen volume is difficult to interpret because FiO2 varies depending on the method of oxygen delivery.
| null | null |
jcm11051247_makarova
| 0 |
The impact of Tocilizumab on radiological changes can not be adequately analyzed without a control group not receiving the drug.
| null | null |
1 Response to Reviewer 2 Comments Point 1: 2.
| null | null |
jcm11051247_makarova
| 0 |
Results 86 cases are excluded from 187 cases, which is too many.
| null | null |
The median oxygen flow rate is shown to be 14 L/min, but FiO2 may vary depending on the method of oxygen delivery (nasal canulae, HNF, MV).
| null | null |
jcm11051247_makarova
| 0 |
Timing of TOC administration according to the oxygen flow rates As mentioned above, the cutoff value is O2 13L/min, but the oxygen volume is difficult to interpret because FiO2 varies depending on the method of oxygen delivery.
| null | null |
When comparing different oxygen delivery methods, isn't it more appropriate to compare FiO2 rather than oxygen flow rate?
| null | null |
jcm11051247_makarova
| 0 |
1 Response to Reviewer 1 Comments Point 1: This study has a design flaw.
| null | null |
Point 2: The proposed timing of Tocilizumab administration based on oxygen flow rate is interesting but undoubtedly requires a more detailed statistical analysis to convince the readers.
| null | null |
jcm11051247_makarova
| 0 |
Author Response Please see the attachment Author Response File: Author Response.pdf
| null | null |
Study design and population If there are mutations or differences in viral strains during the course of the study, should differences in response to TOC and timing of administration depending on the strain be considered?
| null | null |
jcm11051247_makarova
| 0 |
Thank you for improving this paper, especially regarding the timing of TOC administration. Still,
| null | null |
Timing of TOC administration according to the oxygen flow rates As mentioned above, the cutoff value is O2 13L/min, but the oxygen volume is difficult to interpret because FiO2 varies depending on the method of oxygen delivery.
| null | null |
jcm11051247_makarova
| 0 |
Results 2 The oxygen flow rate of 13 L/min measured by ROC as a cutoff value for TOC administration should be presented by FiO2.
| null | null |
Comparative radiologic changes before and after TOC administration In Table 2, about the change in CT findings in fatal and non-fatal patients before and after TOC administration, isn't it the same as the difference in imaging findings between patients who survive after severe illness and those who do not, regardless of TOC?
| null | null |
jcm11051247_makarova
| 0 |
The introduction part can highlight the key points and innovations of this paper.
| null | null |
It is recommended that the number of digits after the decimal point for the value of the correlation coefficient throughout the text be consistent.
| null | null |
land11030363_makarova
| 0 |
Table 1: The pH values are different from those in the previous version.
| null | null |
Note the singular and plural in the sentence.
| null | null |
land11030363_makarova
| 0 |
My major concerns: This manuscript attempts to integrate different preprocessing methods (8 kinds), sample grouping designs (88/26, 75%/25%), and soil spectral data to predict 15 soil properties by building PLSR models.
| null | null |
“Materials & Method" section: a) There is not adequate information regarding quality control of the chemical analyses.
| null | null |
land11030363_makarova
| 0 |
However, there are some issues that need to be resolved before this manuscript can be considered for publication.
| null | null |
Generally, soil spectral measurement is carried out in a dark room to avoid interference from external light sources.
| null | null |
land11030363_makarova
| 0 |
Reference to formal analytical methods must be made in a scientific manner.
| null | null |
Although this manuscript is about the application of proximal remote sensing technology to the prediction of soil properties in mountainous areas, the research focus of this manuscript is not prominent, and the conclusions drawn are not very clear.
| null | null |
land11030363_makarova
| 0 |
It would be wise to give more international flavor in the sections of Introduction and Discussion.
| null | null |
Should the writing of the results and discussion sections be separated?
| null | null |
land11030363_makarova
| 0 |
6) Discussion with new 3 subheading was significantly expanded and linked to the references, and the novelty was highlighted.
| null | null |
Line 264: “…, and hence parent material.” It’s not clear here.
| null | null |
land11030363_makarova
| 0 |
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