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According to your suggestion, we have modified the literature review and included some latest references i.e., [22][29][30][32]. In addition, we organized the literature review in more comprehensive way.
| 2 | 1 |
A comprehensive deeper literature review is necessary to address the research issue. Also, authors need to provide a literature survey in an organized way.-
| 1 | 2 |
en15031006_perova
| 1 |
According to your suggestion, we have improved the Figures as can be seen in the revised version of the manuscript.
| 2 | 1 |
All the figures need to be improved.
| 1 | 2 |
en15031006_perova
| 1 |
We have taken your advice and changed statistical methods in the manuscript.
| 2 | 1 |
English language should be improved.
| 1 | 2 |
fermentation8050212_makarova
| 1 |
We have improved English language.
| 2 | 1 |
Sometimes the document is different to follow due to English issues. Examples: Page 1, Lines 9-11: "In order to enhancement the antioxidant properties of rice bran, solid state fermentation of rice bran with mixed bacteria was adopted and optimized fermentation technology. "; Page 4, Lines 141-143: “In the morning (on set light), breeding 9 fishes interbreed 1h, embryos were obtained from natural spawning collection of embryos were completes in petri Dishes; Page 4, Lines 153-155: “AAPH group only added AAPH. Four experimental groups were added AAPH and 0.1, 0.2, 0.3, 0.4 mg/ mL sample, respectively.”; Page 5, Lines 188-190: “The optimum sodium humate content was found to be 1.0 %, which DPPH free radical scavenging rate of 81.22%.”; Page 6, Lines 218-219: “The significantly less polysaccharide content of FRBE than that of RBE (P＜05).”; Page 8, Lines 241-242: “Schmidt et al. (2014) also found that ferulic acid content of rice bran water-soluble extracts significant improvements, up to 765 mg/g [16].”; Page 10, Lines 321-323: “At the concentration of 0.3 mg/mL and 0.4 mg/mL, CAT activity induced by AAPH of FRBE group significantly higher than that of AAPH and RBE.”.
| 1 | 2 |
fermentation8050212_makarova
| 1 |
FRBE is water-soluble fermented rice bran extracts.
| 2 | 1 |
Explain what FRBE is. It appears for the first time on Page 1, Line 17; Response: Thanks for your comment.
| 1 | 2 |
fermentation8050212_makarova
| 1 |
The hot water extraction conditions was adopt according to previous methods (Dong et al., 2021). Dong, C.L. ; Liu, N.; Wang, Y.; Song, M.; Wang, R.F. ; Yang, Y.P. ; An, X.P. Study on Antioxidant Activity of Fermented Rice Bran Water Extract. Feed Industry 2021, 42(24), 8-13. DOI:10.13302/j.cnki.fi.2021.24.002.
| 2 | 1 |
The hot water extraction conditions were tested before?
| 1 | 2 |
fermentation8050212_makarova
| 1 |
24hpf means 24 hours post fertilization.
| 2 | 1 |
What do the authors mean by 24hpf (Page 4, Line 148)?
| 1 | 2 |
fermentation8050212_makarova
| 1 |
AO solution is acridine orange.
| 2 | 1 |
Explain what AO solution is. It appears on Page 4, Line 162; Response: Thanks for your comment.
| 1 | 2 |
fermentation8050212_makarova
| 1 |
; Qi, J.W. Humates added to feed stimulate the microbial growth(Huck et al., 1991). Sodium humate added to fermentation substrate improved the growth of bacteria (Liu et al., 2014, Shi et al., 2016). The role of sodium humate is fermentation synergist. Huck, T.A., N. Porter and M.E. Bushell, 1991. Effect of humates on microbial activity. Gen. Microbiol. 137: 2321-2329. Liu, N.; An, X.P. ; Tong, B. S.; Chen, D. Y.; Yu, C.Q. Study on the effect of sodium humate on solid fermentation of lactobacillus plantarum. Chinese Journal of Animal Science 2014, 50(05),79-82. Shi, J.X. ; Qi, J.W; An, X.P. ; Liu, N.; Luo, X.G; Chen, D.Y. The influence of sodium humate on fermentation characteristics of yeast. Feed Industry, 2016, 37(04), 44-47.
| 2 | 1 |
Explain the role of sodium humate.
| 1 | 2 |
fermentation8050212_makarova
| 1 |
It is my mistake to write the water-soluble polysaccharide content of FRBE and RBE on the wrong sides. The water-soluble polysaccharide content values have been corrected.
| 2 | 1 |
Page 6, Lines 217-218: Check the values presented in the following sentence: “As shown in Table 1, the water-soluble polysaccharide content of FRBE and RBE were 552.30±2.68 mg g-1 and 450.28±1.69 mg g-1 respectively.”, because they are opposite to the ones presented in Table 1.
| 1 | 2 |
fermentation8050212_makarova
| 1 |
We have taken your advice to change the number of decimal places.
| 2 | 1 |
Table 1: The number of decimal places of some constituents is excessive. Example: for polysaccharide content, I suggest writing 552+3 for RBE.
| 1 | 2 |
fermentation8050212_makarova
| 1 |
Phenolic acids belongs to polyphenols. The increasing of content of water-soluble phenolic acids in FRBE may be attributed to the degradation of rice bran cell wall during fermentation.
| 2 | 1 |
Why did the water-soluble phenolic acids contents increase after the fermentation?
| 1 | 2 |
fermentation8050212_makarova
| 1 |
Fig.3a and 3b showing different groups of LPO rate.
| 2 | 1 |
Page 9, Line 280: Is Fig. 3b showing different groups of LPO rate, or is Fig.3a?
| 1 | 2 |
fermentation8050212_makarova
| 1 |
The 73.85 and 77.00 values are correct.
| 2 | 1 |
Page 10, Lines 306 and 307: check the 73.85 and 77.00 values. They seem interchanged with each other.
| 1 | 2 |
fermentation8050212_makarova
| 1 |
We have rewrited the first sentence in Conclusions. In summary, the DPPH radical radical scavenging abilities of rice bran water-soluble extracts and the content of bioactive constituents were considerably altered by mixed bacteria solid-state fermentation.
| 2 | 1 |
Rewrite the first sentence in Conclusions.
| 1 | 2 |
fermentation8050212_makarova
| 1 |
I have changed.
| 2 | 1 |
Page 8, Line 261: Change “FRB” to “FRBE”.
| 1 | 2 |
fermentation8050212_makarova
| 1 |
The abbreviated form of some journal titles have been corrected to full form taking into account the rules of the journal.
| 2 | 1 |
In the reference list, write the names of the microorganisms in italic. Some journal titles are registered in abbreviated form, and others have a full name. Standardize, taking into account the rules of the journal.
| 1 | 2 |
fermentation8050212_makarova
| 1 |
The statistical analysis were conducted as follows: All experiments were conducted in triplicate. The UNIVARIATE procedure of SAS was used to test the normality of the data before any further analyses were carried out. Data were subjected to ANOVA using the MIXED procedure of SAS (SAS 9.2, SAS Institute Inc., Cary, NC). Mean ± standard error (S.E.) were reported, and means were separated by LSD multiple comparisons. Differences were accepted as statistically significance at P < 0.05.
| 2 | 1 |
The main comment addressing the article improvement is the methodology applied for statistical analysis. The appropriate methodology considering parametric assumption for normality and homogeneity of variances before data analysis should be applied. Later one-factor analysis of variance should carry out accordingly. The application of the Duncan test should be justified because it is less sensitive in this kind of study.
| 1 | 2 |
fermentation8050212_makarova
| 1 |
Thanks for your comment. We have enhanced clarity and detail of the image for easy reading.
| 2 | 1 |
Figures 2a, 3a and 4a include photos whose resolution should be improved.
| 1 | 2 |
fermentation8050212_makarova
| 1 |
We have taken your advice and changed statistical methods in the manuscript.
| 2 | 1 |
please include a more specific section about the statistical methods.
| 1 | 2 |
fermentation8050212_perova
| 1 |
We have improved English language.
| 2 | 1 |
Sometimes the document is different to follow due to English issues. Examples: Page 1, Lines 9-11: "In order to enhancement the antioxidant properties of rice bran, solid state fermentation of rice bran with mixed bacteria was adopted and optimized fermentation technology. "; Page 4, Lines 141-143: “In the morning (on set light), breeding 9 fishes interbreed 1h, embryos were obtained from natural spawning collection of embryos were completes in petri Dishes; Page 4, Lines 153-155: “AAPH group only added AAPH. Four experimental groups were added AAPH and 0.1, 0.2, 0.3, 0.4 mg/ mL sample, respectively.”; Page 5, Lines 188-190: “The optimum sodium humate content was found to be 1.0 %, which DPPH free radical scavenging rate of 81.22%.”; Page 6, Lines 218-219: “The significantly less polysaccharide content of FRBE than that of RBE (P＜05).”; Page 8, Lines 241-242: “Schmidt et al. (2014) also found that ferulic acid content of rice bran water-soluble extracts significant improvements, up to 765 mg/g [16].”; Page 10, Lines 321-323: “At the concentration of 0.3 mg/mL and 0.4 mg/mL, CAT activity induced by AAPH of FRBE group significantly higher than that of AAPH and RBE.”.
| 1 | 2 |
fermentation8050212_perova
| 1 |
FRBE is water-soluble fermented rice bran extracts.
| 2 | 1 |
Explain what FRBE is. It appears for the first time on Page 1, Line 17; Response: Thanks for your comment.
| 1 | 2 |
fermentation8050212_perova
| 1 |
The hot water extraction conditions was adopt according to previous methods (Dong et al., 2021). Dong, C.L. ; Liu, N.; Wang, Y.; Song, M.; Wang, R.F. ; Yang, Y.P. ; An, X.P. ; Qi, J.W. Study on Antioxidant Activity of Fermented Rice Bran Water Extract. Feed Industry 2021, 42(24), 8-13. DOI:10.13302/j.cnki.fi.2021.24.002.
| 2 | 1 |
The hot water extraction conditions were tested before?
| 1 | 2 |
fermentation8050212_perova
| 1 |
24hpf means 24 hours post fertilization.
| 2 | 1 |
What do the authors mean by 24hpf (Page 4, Line 148)?
| 1 | 2 |
fermentation8050212_perova
| 1 |
AO solution is acridine orange.
| 2 | 1 |
Explain what AO solution is. It appears on Page 4, Line 162; Response: Thanks for your comment.
| 1 | 2 |
fermentation8050212_perova
| 1 |
Humates added to feed stimulate the microbial growth(Huck et al., 1991). Sodium humate added to fermentation substrate improved the growth of bacteria (Liu et al., 2014, Shi et al., 2016). The role of sodium humate is fermentation synergist.
| 2 | 1 |
Explain the role of sodium humate.
| 1 | 2 |
fermentation8050212_perova
| 1 |
It is my mistake to write the water-soluble polysaccharide content of FRBE and RBE on the wrong sides. The water-soluble polysaccharide content values have been corrected.
| 2 | 1 |
Page 6, Lines 217-218: Check the values presented in the following sentence: “As shown in Table 1, the water-soluble polysaccharide content of FRBE and RBE were 552.30±2.68 mg g-1 and 450.28±1.69 mg g-1 respectively.”, because they are opposite to the ones presented in Table 1.
| 1 | 2 |
fermentation8050212_perova
| 1 |
We have taken your advice to change the number of decimal places.
| 2 | 1 |
Table 1: The number of decimal places of some constituents is excessive. Example: for polysaccharide content, I suggest writing 552+3 for RBE.
| 1 | 2 |
fermentation8050212_perova
| 1 |
Phenolic acids belongs to polyphenols. The increasing of content of water-soluble phenolic acids in FRBE may be attributed to the degradation of rice bran cell wall during fermentation.
| 2 | 1 |
Why did the water-soluble phenolic acids contents increase after the fermentation?
| 1 | 2 |
fermentation8050212_perova
| 1 |
Fig.3a and 3b showing different groups of LPO rate.
| 2 | 1 |
Page 9, Line 280: Is Fig. 3b showing different groups of LPO rate, or is Fig.3a?
| 1 | 2 |
fermentation8050212_perova
| 1 |
The 73.85 and 77.00 values are correct.
| 2 | 1 |
Page 10, Lines 306 and 307: check the 73.85 and 77.00 values. They seem interchanged with each other.
| 1 | 2 |
fermentation8050212_perova
| 1 |
We have rewrited the first sentence in Conclusions. In summary, the DPPH radical radical scavenging abilities of rice bran water-soluble extracts and the content of bioactive constituents were considerably altered by mixed bacteria solid-state fermentation.
| 2 | 1 |
Rewrite the first sentence in Conclusions.
| 1 | 2 |
fermentation8050212_perova
| 1 |
The “oscillated for 10 minutes” means “shaked for 10 minutes”.
| 2 | 1 |
Page 3, Line 124: What do you mean “oscillated for 10 minutes”? Is mixing for 10 minutes?
| 1 | 2 |
fermentation8050212_perova
| 1 |
I have changed.
| 2 | 1 |
Page 8, Line 261: Change “FRB” to “FRBE”.
| 1 | 2 |
fermentation8050212_perova
| 1 |
The abbreviated form of some journal titles have been corrected to full form taking into account the rules of the journal.
| 2 | 1 |
In the reference list, write the names of the microorganisms in italic. Some journal titles are registered in abbreviated form, and others have a full name. Standardize, taking into account the rules of the journal.
| 1 | 2 |
fermentation8050212_perova
| 1 |
The statistical analysis were conducted as follows: All experiments were conducted in triplicate. The UNIVARIATE procedure of SAS was used to test the normality of the data before any further analyses were carried out. Data were subjected to ANOVA using the MIXED procedure of SAS (SAS 9.2, SAS Institute Inc., Cary, NC). Mean ± standard error (S.E.) were reported, and means were separated by LSD multiple comparisons. Differences were accepted as statistically significance at P < 0.05.
| 2 | 1 |
The main comment addressing the article improvement is the methodology applied for statistical analysis. The appropriate methodology considering parametric assumption for normality and homogeneity of variances before data analysis should be applied. Later one-factor analysis of variance should carry out accordingly. The application of the Duncan test should be justified because it is less sensitive in this kind of study.
| 1 | 2 |
fermentation8050212_perova
| 1 |
Thanks for your comment. We have enhanced clarity and detail of the image for easy reading.
| 2 | 1 |
Figures 2a, 3a and 4a include photos whose resolution should be improved.
| 1 | 2 |
fermentation8050212_perova
| 1 |
Thanks for your suggestion. In this study, texture profile analysis was investigated by a Texture Analyzer (TA.XT PLUS/50, STABLEMICVO, UK) using a P/0.5R cylindrical probe, and corresponding description has been added to the manuscript in Line 526 marked in red font.
| 2 | 1 |
What equipment was used for the texture profile analysis?
| 1 | 2 |
gels8040212_makarova
| 1 |
The gel without the emulsion was taken as the emulsion blank gel, which was the gel formed only by gel continuous phase.
| 2 | 1 |
Describe the emulsion blank gel.
| 1 | 2 |
gels8040212_makarova
| 1 |
Wet gel were the emulsion gels we obtained, which had a three-dimensional network structure that can hold a large amount of water. The corresponding Line 570 in the manuscript has been revised to a more accurate expression, emulsion gels. Thanks again for your careful review.
| 2 | 1 |
What does the term "wet gel" mean?
| 1 | 2 |
gels8040212_makarova
| 1 |
Thanks for carefully checking. Whey protein isolate (WPI) has been added in Line 60-61 in the manuscript.
| 2 | 1 |
Line 60: define WPI at first use.
| 1 | 2 |
gels8040212_makarova
| 1 |
Thanks for your constructive comment. Line 84-88 has been removed and it made the manuscript more logically. Thank you again for the good comment.
| 2 | 1 |
Line 84-88: This is a summary of what was carried out and not a discussion, please remove.
| 1 | 2 |
gels8040212_makarova
| 1 |
The emulsions of different droplet size was prepared at WPI concentration of 1.5 wt% and this information has been added in Line 102. This concentration was selected as a suitable emulsifier concentration based on the results in Figure 1a and 1b, and more details can be found in the manuscript.
| 2 | 1 |
Figure 2: Increase the font of the legend.
| 1 | 2 |
gels8040212_makarova
| 1 |
The manuscript has been revised accordingly in Line 159-161.
| 2 | 1 |
Line 166: English check.
| 1 | 2 |
gels8040212_makarova
| 1 |
Figure 3 has been revised accordingly.
| 2 | 1 |
Figure 3: rename using latin alphabet
| 1 | 2 |
gels8040212_makarova
| 1 |
Figure 4 has been adjusted to make the legend clear. In fact, B1, B2 and B3 were in a relatively homogeneous state, and no phase separation had occurred. The shadow in the middle part of the apparent image of the heat-induced gels was because the texture test was carried out before shooting. Unlike TGase-induced emulsion gel had very good elastic texture, the structure of heat-induced gels did not fully recover to its original state due to its soft and less elastic texture after compression.
| 2 | 1 |
Figure 4: Increase legend as it is not visible. B1, B2, and B3 looks biphasic, was this the case?
| 1 | 2 |
gels8040212_makarova
| 1 |
Thanks for your constructive suggestion. Figure 7B has been revised accordingly, that is, a graph was added which displayed the apparent viscosity of all emulsion gels at a fixed shear rate of 0.25/s, showing the differences between groups very visually. Thanks again for your suggestion.
| 2 | 1 |
Figure 7-B: I suggest choosing just one shear rate, instead of providing both graphs one can provide the numerical values at a fixed shear rate.
| 1 | 2 |
gels8040212_makarova
| 1 |
In order to choose appropriate emulsifier, we prepare different WPI concentration of 1.0-2.0 wt% and found the emulsion formulated with 1.5 wt% WPI had smaller size and medimum zeta potential, so it was described to the optimize condition. For clarity, we have also revised the corresponding positions in the manuscript.
| 2 | 1 |
Line 474: What is meant by optimize condition?
| 1 | 2 |
gels8040212_makarova
| 1 |
Thanks for your good suggestion. The gel continuous phase was the WPI-gelatin mixture. To more clearly describe the composition of the emulsion gel, Table 3 has been added to the manuscript. The pre-gel mixture, refered to the mixture before gelation occurred, was composed of WPI-gelatin mixture and emulsions, but due to the large number of samples prepared by the single factor experiment, only the general formulation of the emulsion gel was listed in Table 3. And the relevant statements in 4.2.2 and 4.2.3 have been revised in the manuscript.
| 2 | 1 |
Line 478 and 485: Are the gel continuous phase and the protein-emulsion mixture the same thing? This section is a bit confusing so I would suggest authors include a Table with all the sample formulations/conditions tested.
| 1 | 2 |
gels8040212_makarova
| 1 |
Thanks for your check. It was revised in the manuscript.
| 2 | 1 |
Line 496: remove respectively
| 1 | 2 |
gels8040212_makarova
| 1 |
Thanks for your carefully review. The characterization of emulsions was investigated by the droplet size, polydispersity index (PDI) and zeta potential. This expression has been revised in the manuscript. Point 14: Line 500: Define PDI at first use
| 2 | 1 |
Line 499. Characteristics or characterization?
| 1 | 2 |
gels8040212_makarova
| 1 |
PDI was the polydispersity index and the manuscript has been revised in Line 501.
| 2 | 1 |
Line 500: Define PDI at first use Line 507: Clearance height or gap`?
| 1 | 2 |
gels8040212_makarova
| 1 |
The gap was set to 1 mm. Thank you again for your careful review.
| 2 | 1 |
Line 507: Clearance height or gap`?
| 1 | 2 |
gels8040212_makarova
| 1 |
Thanks for your question. 1% strain was within the linear viscoelastic region in our pre-experiment so it was chosen for other tests.
| 2 | 1 |
Line 512: Was 1% found to be within the viscoelastic region?
| 1 | 2 |
gels8040212_makarova
| 1 |
Thanks for your comment. The statement in 4.2.5 has been revised in the manuscript.
| 2 | 1 |
Line 516: rephase
| 1 | 2 |
gels8040212_makarova
| 1 |
Thanks for your carefully check. Line 533 has been modified which corresponding to the line of revised manuscript.
| 2 | 1 |
Line 530: English check
| 1 | 2 |
gels8040212_makarova
| 1 |
Thanks for your suggestion. In this study, texture profile analysis was investigated by a Texture Analyzer (TA.XT PLUS/50, STABLEMICVO, UK) using a P/0.5R cylindrical probe, and corresponding description has been added to the manuscript in Line 526 marked in red font.
| 2 | 1 |
What equipment was used for the texture profile analysis?
| 1 | 2 |
gels8040212_perova
| 1 |
The gel without the emulsion was taken as the emulsion blank gel, which was the gel formed only by gel continuous phase.
| 2 | 1 |
Describe the emulsion blank gel.
| 1 | 2 |
gels8040212_perova
| 1 |
Wet gel were the emulsion gels we obtained, which had a three-dimensional network structure that can hold a large amount of water. The corresponding Line 570 in the manuscript has been revised to a more accurate expression, emulsion gels.
| 2 | 1 |
What does the term "wet gel" mean?
| 1 | 2 |
gels8040212_perova
| 1 |
It is our great pleasure to get your professional comments. Thanks very much for your contribution to our manuscript. We take the concerns seriously and comments have been carefully addressed.
| 2 | 1 |
One main request would be to make it clearer what was the formulations and conditions tested.
| 1 | 2 |
gels8040212_perova
| 1 |
Thanks for carefully checking. Whey protein isolate (WPI) has been added in Line 60-61 in the manuscript.
| 2 | 1 |
Line 60: define WPI at first use.
| 1 | 2 |
gels8040212_perova
| 1 |
Thanks for your constructive comment. Line 84-88 has been removed and it made the manuscript more logically. Thank you again for the good comment.
| 2 | 1 |
Line 84-88: This is a summary of what was carried out and not a discussion, please remove.
| 1 | 2 |
gels8040212_perova
| 1 |
The emulsions of different droplet size was prepared at WPI concentration of 1.5 wt% and this information has been added in Line 102. This concentration was selected as a suitable emulsifier concentration based on the results in Figure 1a and 1b, and more details can be found in the manuscript.
| 2 | 1 |
Figure 1: Include in Fig.1c what was the the % of WPI used.
| 1 | 2 |
gels8040212_perova
| 1 |
Thanks for your good comment. Figure 2 has been modified accordingly to make the legend clearer.
| 2 | 1 |
Figure 2: Increase the font of the legend.
| 1 | 2 |
gels8040212_perova
| 1 |
The manuscript has been revised accordingly in Line 159-161.
| 2 | 1 |
Line 166: English check.
| 1 | 2 |
gels8040212_perova
| 1 |
Figure 3 has been revised accordingly.
| 2 | 1 |
Figure 3: rename using latin alphabet
| 1 | 2 |
gels8040212_perova
| 1 |
Figure 4 has been adjusted to make the legend clear. In fact, B1, B2 and B3 were in a relatively homogeneous state, and no phase separation had occurred. The shadow in the middle part of the apparent image of the heat-induced gels was because the texture test was carried out before shooting. Unlike TGase-induced emulsion gel had very good elastic texture, the structure of heat-induced gels did not fully recover to its original state due to its soft and less elastic texture after compression.
| 2 | 1 |
Figure 4: Increase legend as it is not visible. B1, B2, and B3 looks biphasic, was this the case?
| 1 | 2 |
gels8040212_perova
| 1 |
Thanks for your constructive suggestion. Figure 7B has been revised accordingly, that is, a graph was added which displayed the apparent viscosity of all emulsion gels at a fixed shear rate of 0.25/s, showing the differences between groups very visually. Thanks again for your suggestion.
| 2 | 1 |
Figure 7-B: I suggest choosing just one shear rate, instead of providing both graphs one can provide the numerical values at a fixed shear rate.
| 1 | 2 |
gels8040212_perova
| 1 |
In order to choose appropriate emulsifier, we prepare different WPI concentration of 1.0-2.0 wt% and found the emulsion formulated with 1.5 wt% WPI had smaller size and medimum zeta potential, so it was described to the optimize condition. For clarity, we have also revised the corresponding positions in the manuscript.
| 2 | 1 |
Line 474: What is meant by optimize condition?
| 1 | 2 |
gels8040212_perova
| 1 |
Thanks for your good suggestion. The gel continuous phase was the WPI-gelatin mixture. To more clearly describe the composition of the emulsion gel, Table 3 has been added to the manuscript. The pre-gel mixture, refered to the mixture before gelation occurred, was composed of WPI-gelatin mixture and emulsions, but due to the large number of samples prepared by the single factor experiment, only the general formulation of the emulsion gel was listed in Table 3. And the relevant statements in 4.2.2 and 4.2.3 have been revised in the manuscript.
| 2 | 1 |
Line 478 and 485: Are the gel continuous phase and the protein-emulsion mixture the same thing? This section is a bit confusing so I would suggest authors include a Table with all the sample formulations/conditions tested.
| 1 | 2 |
gels8040212_perova
| 1 |
Thanks for your check. It was revised in the manuscript.
| 2 | 1 |
Line 496: remove respectively
| 1 | 2 |
gels8040212_perova
| 1 |
Thanks for your carefully review. The characterization of emulsions was investigated by the droplet size, polydispersity index (PDI) and zeta potential. This expression has been revised in the manuscript.
| 2 | 1 |
Line 499. Characteristics or characterization?
| 1 | 2 |
gels8040212_perova
| 1 |
PDI was the polydispersity index and the manuscript has been revised in Line 501.
| 2 | 1 |
Line 500: Define PDI at first use
| 1 | 2 |
gels8040212_perova
| 1 |
The gap was set to 1 mm. Thank you again for your careful review.
| 2 | 1 |
Line 507: Clearance height or gap`?
| 1 | 2 |
gels8040212_perova
| 1 |
Thanks for your question. 1% strain was within the linear viscoelastic region in our pre-experiment so it was chosen for other tests.
| 2 | 1 |
Line 512: Was 1% found to be within the viscoelastic region?
| 1 | 2 |
gels8040212_perova
| 1 |
Thanks for your comment. The statement in 4.2.5 has been revised in the manuscript.
| 2 | 1 |
Line 516: rephase
| 1 | 2 |
gels8040212_perova
| 1 |
Thanks for your carefully check. Line 533 has been modified which corresponding to the line of revised manuscript.
| 2 | 1 |
Line 530: English check
| 1 | 2 |
gels8040212_perova
| 1 |
Thanks for your good suggestion and we have improved it.
| 2 | 1 |
The authors look at the expression of IbTCP11/17 (targets of miR319) from lines 418-424. Here authors capture the early response to PEG compared to the drought response (water withdrawal) assessed earlier. To have a meaningful comparison, the authors need to show the expression level of these two genes in the drought assay (Lines 400-417). As the authors briefly mentioned, PEG stress is more severe than water withdrawal, so it will not give a solid verification provided there are two variables, inducer, and timing. In our experience, we observed completely different responses when we tried to compare PEG, air drying, and water withdrawal. This is essential given that no temporal expression baseline is established for IbTCP11/17 under water withdrawal stress and the presence of other regulatory mechanisms of IbTCP11/17.
| 1 | 2 |
genes13030404_makarova
| 1 |
Thanks for your good suggestion and we have added the possible reason in the discussion.
| 2 | 1 |
It is interesting to see that only MDA content increases in response to drought. The possible mechanism/reason should be included in the discussion.
| 1 | 2 |
genes13030404_makarova
| 1 |
Thank you for your positive and constructive suggestions and we have improved it in our revised manuscript.
| 2 | 1 |
The discussion needs to be further expanded. Esp. RNA-Seq results need to be further discussed.
| 1 | 2 |
genes13030404_makarova
| 1 |
Thanks for your good suggestions on this point. In our revised manuscript, we have made corresponding modifications to summary our work.
| 2 | 1 |
There should be a summary (concluding statement at the end). This manuscript ends very abruptly.
| 1 | 2 |
genes13030404_makarova
| 1 |
Thanks for your good suggestion. We removed “and so on” in our revised manuscript.
| 2 | 1 |
Pg 1 line 45: the wording "and so on" is awkward and imprecise. Please rephrase and indicate what other processes are being referred to.
| 1 | 2 |
genes13030404_makarova
| 1 |
Thanks for your reminding. We modified “As we all know” into “More and more research reveals that”.
| 2 | 1 |
Pg 2 line 67: don't assume that everyone reading the manuscript are aware of miRNA roles in plant stress physiology.
| 1 | 2 |
genes13030404_makarova
| 1 |
It is really true as you mention that the details of methods is needed. But considering that this method is also expressed in thus way in our previous articles (Ren L, et al (2021) Genome-Wide Identification of TCP Transcription Factors Family in Sweet Potato Reveals Significant Roles of miR319-Targeted TCPs in Leaf Anatomical Morphology. Front. Plant Sci. 12: 686698. doi: 10.3389/fpls.2021.686698), we just cite the original source here. Point 4: Pg 6 line 239: I don't understand the point being made here.
| 2 | 1 |
Pg 3 Plasmid and sweet potato genetic transformation section: Include relevant protocols instead of just referring to another manuscript. Citing the original source is required but so are details needed to reproduce and evaluate the soundness of the manuscript.
| 1 | 2 |
genes13030404_makarova
| 1 |
Thanks for your question Target mimicry is a common method to study the function of miRNA. There is a non-coding protein gene IPS in Arabidopsis, and its transcription RNA can specifically bind to miRNA through sequence complementarity, but it forms incomplete complementary bubble structure at the cleavage site of miRNA, that is, miRNA cannot cleavage IPS RNA, thus inhibiting the degradation of miRNA to its target gene mRNA. IPS gene is equivalent to the target mimicry. (Franco-Zorrilla et al., 2007) 2 1
| 2 | 1 |
Pg 6 line 239: I don't understand the point being made here. "sequester the normal expression" is nonsensical. Please clarify and rephrase.
| 1 | 2 |
genes13030404_makarova
| 1 |
Thanks for your good suggestion and we have improved it.
| 2 | 1 |
The authors look at the expression of IbTCP11/17 (targets of miR319) from lines 418-424. Here authors capture the early response to PEG compared to the drought response (water withdrawal) assessed earlier. To have a meaningful comparison, the authors need to show the expression level of these two genes in the drought assay (Lines 400-417). As the authors briefly mentioned, PEG stress is more severe than water withdrawal, so it will not give a solid verification provided there are two variables, inducer, and timing. In our experience, we observed completely different responses when we tried to compare PEG, air drying, and water withdrawal. This is essential given that no temporal expression baseline is established for IbTCP11/17 under water withdrawal stress and the presence of other regulatory mechanisms of IbTCP11/17.
| 1 | 2 |
genes13030404_perova
| 1 |
Thanks for your good suggestion and we have added the possible reason in the discussion.
| 2 | 1 |
It is interesting to see that only MDA content increases in response to drought. The possible mechanism/reason should be included in the discussion.
| 1 | 2 |
genes13030404_perova
| 1 |
Thank you for your positive and constructive suggestions and we have improved it in our revised manuscript.
| 2 | 1 |
The discussion needs to be further expanded. Esp. RNA-Seq results need to be further discussed.
| 1 | 2 |
genes13030404_perova
| 1 |
Thanks for your good suggestions on this point. In our revised manuscript, we have made corresponding modifications to summary our work.
| 2 | 1 |
There should be a summary (concluding statement at the end). This manuscript ends very abruptly.
| 1 | 2 |
genes13030404_perova
| 1 |
Thanks for your good suggestion. We removed “and so on” in our revised manuscript.
| 2 | 1 |
Pg 1 line 45: the wording "and so on" is awkward and imprecise. Please rephrase and indicate what other processes are being referred to.
| 1 | 2 |
genes13030404_perova
| 1 |
Thanks for your reminding. We modified “As we all know” into “More and more research reveals that”.
| 2 | 1 |
Pg 2 line 67: don't assume that everyone reading the manuscript are aware of miRNA roles in plant stress physiology.
| 1 | 2 |
genes13030404_perova
| 1 |
It is really true as you mention that the details of methods is needed. But considering that this method is also expressed in thus way in our previous articles (Ren L, et al (2021) Genome-Wide Identification of TCP Transcription Factors Family in Sweet Potato Reveals Significant Roles of miR319-Targeted TCPs in Leaf Anatomical Morphology. Front. Plant Sci. 12: 686698. doi: 10.3389/fpls.2021.686698), we just cite the original source here.
| 2 | 1 |
Pg 3 Plasmid and sweet potato genetic transformation section: Include relevant protocols instead of just referring to another manuscript. Citing the original source is required but so are details needed to reproduce and evaluate the soundness of the manuscript. The same applies to other parts of the materials and methods:
| 1 | 2 |
genes13030404_perova
| 1 |
Thanks for your question Target mimicry is a common method to study the function of miRNA. There is a non-coding protein gene IPS in Arabidopsis, and its transcription RNA can specifically bind to miRNA through sequence complementarity, but it forms incomplete complementary bubble structure at the cleavage site of miRNA, that is, miRNA cannot cleavage IPS RNA, thus inhibiting the degradation of miRNA to its target gene mRNA. IPS gene is equivalent to the target mimicry. (Franco-Zorrilla et al., 2007) 2 1
| 2 | 1 |
Pg 6 line 239: I don't understand the point being made here. "sequester the normal expression" is nonsensical. Please clarify and rephrase.
| 1 | 2 |
genes13030404_perova
| 1 |
Comments #1 (gene expression assay) and #3 (proper discussion on RNA-Seq data) aren't properly addressed.
| 3 | 2 |
Thank you for your positive and constructive suggestions and we have improved it in our revised manuscript.
| 2 | 1 |
genes13030404_perova
| 1 |
The colour refer to the SEEBASE depth to basement, the legend has been added • Figure 2: The left spine (or border) does not appear on the pdf reviewed.
| 2 | 1 |
Figures. Figure 1: Are colors in Figure 1 A related to the hydrostratigraphy or depicting topography? If the latter applies, make sure to provide a scale.
| 1 | 2 |
geosciences12010037_makarova
| 1 |
Thanks, the figure have been entirely revisited and divided into four subfigures.
| 2 | 1 |
Figure 2: The left spine (or border) does not appear on the pdf reviewed. This figure could be improved by adding shaded satellite image to help the reader compare the mapped drainage systems (which were automatically mapped) and fault traces/lineaments with the real features observed.
| 1 | 2 |
geosciences12010037_makarova
| 1 |
The figure has been redrawn and the magnetic lineaments indicated • Figure 4: This figure could go in the appendix.
| 2 | 1 |
Figure 3: The text (lines 258-259) mentions magnetic lineaments as prominent features, which can be caused by faulting. Such features are hard to visualize. It would certainly help if they were highlighted directly on the figure.
| 1 | 2 |
geosciences12010037_makarova
| 1 |
Thank you. We hesitated on this point but decided to keep the figure in as the quality of the seismic in the shallow level is really crucial to the discussion. We need to know if the interpretation is reliable, especially in this shallow zone that is not well imaged by this type of data. A misinterpretation can led to the targeting of circulation pathways that are in fact only artefacts.
| 2 | 1 |
Figure 4: This figure could go in the appendix. It is good to let the reader know that not all seismic surveys provided the same quality, but this is more a technical point.
| 1 | 2 |
geosciences12010037_makarova
| 1 |
We removed the seismic interpretation of figure 7G (cross) that were misleading the reader and add the interpretation of the fluid leakage features on the images
| 2 | 1 |
Figure 7: What is special about the locations highlighted by the markers (crosses on Figure 7B and square markers on Figure 7C). At this scale, the reader can’t see much detail.
| 1 | 2 |
geosciences12010037_makarova
| 1 |
this is due to the different seismic acquisition technics and processing applied in each survey as well as the fact that our interval of interest is shallow and not fitting with the interval of interest the companies tried to best image with those datasets”
| 2 | 1 |
Lines 271-272: Why is the signal quality so variable, and how were the categories (excellent, average and poor) defined?
| 1 | 2 |
geosciences12010037_makarova
| 1 |
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