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PMC9429313_Fig1_389781.jpg | Describe the main subject of this image. | The sequestrated lung located in the right lower lobe was diagnosed using conventional two-dimensional computed tomography. A The lesion mimics bronchiectasis and infection; B contrast-enhanced images revealed the aberrant feeding vessel |
PMC9429313_Fig2_389785.jpg | Describe the main subject of this image. | Three-dimensional computed tomography angiography showed definitely the anomalous blood supply. A A mass was located in the left lower lobe; B the abnormal feeding artery was next to the spleen artery; C the feeding artery originated from the abdominal aorta was finally confirmed |
PMC9429313_Fig2_389784.jpg | What is shown in this image? | Three-dimensional computed tomography angiography showed definitely the anomalous blood supply. A A mass was located in the left lower lobe; B the abnormal feeding artery was next to the spleen artery; C the feeding artery originated from the abdominal aorta was finally confirmed |
PMC9429316_Fig1_389795.jpg | What is shown in this image? | Pre- and postoperative contrast enhanced computed tomography of left ICA stenosis (white arrow). A Pre-operative axial view, graded 60–70% using NASCET criteria. B Pre-operative sagittal view. C Postoperative near occlusion of the left ICA with postoperative subcutaneous emphysema, axial view. D Postoperative sagittal view |
PMC9429316_Fig1_389797.jpg | What does this image primarily show? | Pre- and postoperative contrast enhanced computed tomography of left ICA stenosis (white arrow). A Pre-operative axial view, graded 60–70% using NASCET criteria. B Pre-operative sagittal view. C Postoperative near occlusion of the left ICA with postoperative subcutaneous emphysema, axial view. D Postoperative sagittal view |
PMC9429316_Fig3_389798.jpg | What is being portrayed in this visual content? | Postoperative computed tomography perfusion imaging showing a large perfusion defect of the entire anterior circulation of the left hemisphere consistent with hypoperfusion due to severe carotid artery stenosis. A Cerebral blood volume. B Cerebral blood flow. C Time to peak. D Mean transit time. Decreased cerebral blood flow with normal cerebral blood volume indicate a small infarct core and large penumbra, e.g. salvageable tissue |
PMC9429316_Fig3_389799.jpg | Describe the main subject of this image. | Postoperative computed tomography perfusion imaging showing a large perfusion defect of the entire anterior circulation of the left hemisphere consistent with hypoperfusion due to severe carotid artery stenosis. A Cerebral blood volume. B Cerebral blood flow. C Time to peak. D Mean transit time. Decreased cerebral blood flow with normal cerebral blood volume indicate a small infarct core and large penumbra, e.g. salvageable tissue |
PMC9429336_Fig1_389804.jpg | What can you see in this picture? | A 26-year-old woman with lower back and left buttock pain for 10 years, aggravating for 1 year. Preoperative MRI showed a SEMC. The dural defect was sutured intraoperatively. One-year follow-up imaging showed no recurrence of the cyst |
PMC9429336_Fig1_389803.jpg | What is the dominant medical problem in this image? | A 26-year-old woman with lower back and left buttock pain for 10 years, aggravating for 1 year. Preoperative MRI showed a SEMC. The dural defect was sutured intraoperatively. One-year follow-up imaging showed no recurrence of the cyst |
PMC9429336_Fig1_389802.jpg | What is the main focus of this visual representation? | A 26-year-old woman with lower back and left buttock pain for 10 years, aggravating for 1 year. Preoperative MRI showed a SEMC. The dural defect was sutured intraoperatively. One-year follow-up imaging showed no recurrence of the cyst |
PMC9429336_Fig3_389808.jpg | What stands out most in this visual? | Several indirect signs on CT findings. A Axial CT revealed the widening of interpedicle distance and thinning of the pedicle. B Axial CT cystography revealed bony scalloping erosion of the posterior edge of the vertebra. Although both the subarachnoid space and the cyst were developed, no cleft was found |
PMC9429336_Fig3_389809.jpg | What does this image primarily show? | Several indirect signs on CT findings. A Axial CT revealed the widening of interpedicle distance and thinning of the pedicle. B Axial CT cystography revealed bony scalloping erosion of the posterior edge of the vertebra. Although both the subarachnoid space and the cyst were developed, no cleft was found |
PMC9429336_Fig4_389811.jpg | Describe the main subject of this image. | Flow void on MRI. A 7-year-old boy had weakness of the lower extremities, with dysuria, for 3 days. A Preoperative sagittal MRI showed a flow void with a SEMC at L1 level. B No flow void was found in axial MRI |
PMC9429336_Fig4_389810.jpg | What is the core subject represented in this visual? | Flow void on MRI. A 7-year-old boy had weakness of the lower extremities, with dysuria, for 3 days. A Preoperative sagittal MRI showed a flow void with a SEMC at L1 level. B No flow void was found in axial MRI |
PMC9429336_Fig5_389805.jpg | What object or scene is depicted here? | Myelography. The difficulty in performing myelography is the timing of the scan after injection. A, B, and C The cyst did not even develop at the time of scan |
PMC9429336_Fig5_389807.jpg | What can you see in this picture? | Myelography. The difficulty in performing myelography is the timing of the scan after injection. A, B, and C The cyst did not even develop at the time of scan |
PMC9429417_Fig15_389815.jpg | What key item or scene is captured in this photo? | Clinical CT scan imaging of 4T1 tumor-bearing mice 6 and 24 h post-injection of either [email protected] or [email protected] |
PMC9429417_Fig15_389816.jpg | What is the central feature of this picture? | Clinical CT scan imaging of 4T1 tumor-bearing mice 6 and 24 h post-injection of either [email protected] or [email protected] |
PMC9429417_Fig15_389813.jpg | What key item or scene is captured in this photo? | Clinical CT scan imaging of 4T1 tumor-bearing mice 6 and 24 h post-injection of either [email protected] or [email protected] |
PMC9429417_Fig15_389812.jpg | What is shown in this image? | Clinical CT scan imaging of 4T1 tumor-bearing mice 6 and 24 h post-injection of either [email protected] or [email protected] |
PMC9429417_Fig15_389814.jpg | Can you identify the primary element in this image? | Clinical CT scan imaging of 4T1 tumor-bearing mice 6 and 24 h post-injection of either [email protected] or [email protected] |
PMC9429417_Fig15_389817.jpg | What is being portrayed in this visual content? | Clinical CT scan imaging of 4T1 tumor-bearing mice 6 and 24 h post-injection of either [email protected] or [email protected] |
PMC9429417_Fig16_389821.jpg | Can you identify the primary element in this image? | The in vivo CT scan coronal view of the tumor site 6 and 24 h post-injection of either [email protected] or [email protected] |
PMC9429417_Fig16_389818.jpg | What stands out most in this visual? | The in vivo CT scan coronal view of the tumor site 6 and 24 h post-injection of either [email protected] or [email protected] |
PMC9429417_Fig16_389820.jpg | What can you see in this picture? | The in vivo CT scan coronal view of the tumor site 6 and 24 h post-injection of either [email protected] or [email protected] |
PMC9429417_Fig16_389823.jpg | What can you see in this picture? | The in vivo CT scan coronal view of the tumor site 6 and 24 h post-injection of either [email protected] or [email protected] |
PMC9429417_Fig16_389822.jpg | Describe the main subject of this image. | The in vivo CT scan coronal view of the tumor site 6 and 24 h post-injection of either [email protected] or [email protected] |
PMC9429453_Fig2_389824.jpg | What is the central feature of this picture? | Slit-lamp photographs of a case of LDAK. a, b Corneal graft is melting with no signs of cellular infiltrations. c, d Corneal graft is re-epithelialized after the treatment of lacrimal drainage pathway disease |
PMC9429453_Fig2_389825.jpg | What is the dominant medical problem in this image? | Slit-lamp photographs of a case of LDAK. a, b Corneal graft is melting with no signs of cellular infiltrations. c, d Corneal graft is re-epithelialized after the treatment of lacrimal drainage pathway disease |
PMC9429453_Fig2_389826.jpg | What can you see in this picture? | Slit-lamp photographs of a case of LDAK. a, b Corneal graft is melting with no signs of cellular infiltrations. c, d Corneal graft is re-epithelialized after the treatment of lacrimal drainage pathway disease |
PMC9429475_Fig1_389828.jpg | What is being portrayed in this visual content? | Digital photography of left chest wall disease for patient 2 before treatment (left) and after the first course of radiation and combination therapy (right), demonstrating dramatic improvement in skin disease |
PMC9429475_Fig2_389831.jpg | Can you identify the primary element in this image? | Axial computed tomography scan of chest for patient 2 before treatment (left) and axial computed tomography scan of patient 2’s chest after treatment with radiation, anti-PD-1, and high-dose IL-2 (right), demonstrating substantial reduction in tumor volume |
PMC9429475_Fig2_389830.jpg | What is the main focus of this visual representation? | Axial computed tomography scan of chest for patient 2 before treatment (left) and axial computed tomography scan of patient 2’s chest after treatment with radiation, anti-PD-1, and high-dose IL-2 (right), demonstrating substantial reduction in tumor volume |
PMC9429485_f0005_389832.jpg | What object or scene is depicted here? | A CT scan demonstrating central cavitation in the superior segment of the left lower lung suggests a septic pulmonary embolism. |
PMC9429582_Fig3_389834.jpg | What object or scene is depicted here? | Labelling of lesions in the elbow joint. a Pig 2. There is a multi-lobulated osteochondrosis lesion at the sagittal ridge of the lateral condylar region, labelled green. b Pig 20. There is a small osteochondrosis lesion at the collateral ligament fossa, labelled green. c Pig 6. There is a cyst-like osteochondrosis lesion in the medial humeral condyle, labelled red. d Pig 31. The arrow points to a mineral hyperdense body adjacent to the proximal radial physis; a previously unreported location for such perichondrial new bone formation, but the lesion is not labelled because it represent physeal, rather than articular osteochondrosis. (Fig. 3d appears smoother because it was exported from the original CT scan via free software [https://horosproject.org/], rather than from the labelling application) |
PMC9429582_Fig3_389833.jpg | What is the central feature of this picture? | Labelling of lesions in the elbow joint. a Pig 2. There is a multi-lobulated osteochondrosis lesion at the sagittal ridge of the lateral condylar region, labelled green. b Pig 20. There is a small osteochondrosis lesion at the collateral ligament fossa, labelled green. c Pig 6. There is a cyst-like osteochondrosis lesion in the medial humeral condyle, labelled red. d Pig 31. The arrow points to a mineral hyperdense body adjacent to the proximal radial physis; a previously unreported location for such perichondrial new bone formation, but the lesion is not labelled because it represent physeal, rather than articular osteochondrosis. (Fig. 3d appears smoother because it was exported from the original CT scan via free software [https://horosproject.org/], rather than from the labelling application) |
PMC9429582_Fig3_389835.jpg | What's the most prominent thing you notice in this picture? | Labelling of lesions in the elbow joint. a Pig 2. There is a multi-lobulated osteochondrosis lesion at the sagittal ridge of the lateral condylar region, labelled green. b Pig 20. There is a small osteochondrosis lesion at the collateral ligament fossa, labelled green. c Pig 6. There is a cyst-like osteochondrosis lesion in the medial humeral condyle, labelled red. d Pig 31. The arrow points to a mineral hyperdense body adjacent to the proximal radial physis; a previously unreported location for such perichondrial new bone formation, but the lesion is not labelled because it represent physeal, rather than articular osteochondrosis. (Fig. 3d appears smoother because it was exported from the original CT scan via free software [https://horosproject.org/], rather than from the labelling application) |
PMC9429582_Fig3_389836.jpg | Describe the main subject of this image. | Labelling of lesions in the elbow joint. a Pig 2. There is a multi-lobulated osteochondrosis lesion at the sagittal ridge of the lateral condylar region, labelled green. b Pig 20. There is a small osteochondrosis lesion at the collateral ligament fossa, labelled green. c Pig 6. There is a cyst-like osteochondrosis lesion in the medial humeral condyle, labelled red. d Pig 31. The arrow points to a mineral hyperdense body adjacent to the proximal radial physis; a previously unreported location for such perichondrial new bone formation, but the lesion is not labelled because it represent physeal, rather than articular osteochondrosis. (Fig. 3d appears smoother because it was exported from the original CT scan via free software [https://horosproject.org/], rather than from the labelling application) |
PMC9429582_Fig4_389840.jpg | What key item or scene is captured in this photo? | Labelling of lesions in the hock joint. a Pig 5. There is an osteochondrosis lesion proximo-dorsally in the medial trochlear ridge of the talus, labelled green. b Pig 10. There is a cyst-like osteochondrosis lesion proximally in the lateral trochlear ridge, labelled blue. c Pig 8. There is an osteochondrosis lesion disto-laterally in the caput of the talus, labelled blue. d Pig 31. The arrow points to a large, irregular and cyst-like defect at the collum of the talus. The defect has deliberately not been labelled as a lesion because there is currently no validated size threshold for distinguishing between normal nutrient foraminae and lesions in this location. (Fig. 4d appears smoother because it was exported from the original CT scan via free software [https://horosproject.org/], rather than from the labelling application) |
PMC9429582_Fig4_389839.jpg | What key item or scene is captured in this photo? | Labelling of lesions in the hock joint. a Pig 5. There is an osteochondrosis lesion proximo-dorsally in the medial trochlear ridge of the talus, labelled green. b Pig 10. There is a cyst-like osteochondrosis lesion proximally in the lateral trochlear ridge, labelled blue. c Pig 8. There is an osteochondrosis lesion disto-laterally in the caput of the talus, labelled blue. d Pig 31. The arrow points to a large, irregular and cyst-like defect at the collum of the talus. The defect has deliberately not been labelled as a lesion because there is currently no validated size threshold for distinguishing between normal nutrient foraminae and lesions in this location. (Fig. 4d appears smoother because it was exported from the original CT scan via free software [https://horosproject.org/], rather than from the labelling application) |
PMC9429582_Fig4_389837.jpg | Can you identify the primary element in this image? | Labelling of lesions in the hock joint. a Pig 5. There is an osteochondrosis lesion proximo-dorsally in the medial trochlear ridge of the talus, labelled green. b Pig 10. There is a cyst-like osteochondrosis lesion proximally in the lateral trochlear ridge, labelled blue. c Pig 8. There is an osteochondrosis lesion disto-laterally in the caput of the talus, labelled blue. d Pig 31. The arrow points to a large, irregular and cyst-like defect at the collum of the talus. The defect has deliberately not been labelled as a lesion because there is currently no validated size threshold for distinguishing between normal nutrient foraminae and lesions in this location. (Fig. 4d appears smoother because it was exported from the original CT scan via free software [https://horosproject.org/], rather than from the labelling application) |
PMC9429625_Fig12_389841.jpg | What's the most prominent thing you notice in this picture? | Representative images of neocortex sections from the FITC-dextran-injected experimental groups, immunolabelled for GFAP. (a, c; cs 1.5) EAE-affected and (b, d; cs 1.5) EAE-affected MSC-treated mice sacrificed at 6 h (6 h) and 24 h (24 h) after MSC treatment. a–d At both time points, the neocortex BBB-microvessels show traces of FITC-dextran leakage as a fluorescent halo in the surrounding parenchyma (a, c; arrows); in contrast, no trace of leakage and diffuse fluorescence is detectable in EAE-affected MSC-treated mice (b, d); note, in the latter, the preserved/restored continuity of the perivascular astrocyte endfeet (b, d; arrowheads) that is apparently lost in untreated EAE-affected mice (a, c; arrowheads). TOPRO-3 nuclear counterstaining. Scale bars: a–f 25 µm |
PMC9429625_Fig12_389842.jpg | What is shown in this image? | Representative images of neocortex sections from the FITC-dextran-injected experimental groups, immunolabelled for GFAP. (a, c; cs 1.5) EAE-affected and (b, d; cs 1.5) EAE-affected MSC-treated mice sacrificed at 6 h (6 h) and 24 h (24 h) after MSC treatment. a–d At both time points, the neocortex BBB-microvessels show traces of FITC-dextran leakage as a fluorescent halo in the surrounding parenchyma (a, c; arrows); in contrast, no trace of leakage and diffuse fluorescence is detectable in EAE-affected MSC-treated mice (b, d); note, in the latter, the preserved/restored continuity of the perivascular astrocyte endfeet (b, d; arrowheads) that is apparently lost in untreated EAE-affected mice (a, c; arrowheads). TOPRO-3 nuclear counterstaining. Scale bars: a–f 25 µm |
PMC9429625_Fig12_389844.jpg | What is being portrayed in this visual content? | Representative images of neocortex sections from the FITC-dextran-injected experimental groups, immunolabelled for GFAP. (a, c; cs 1.5) EAE-affected and (b, d; cs 1.5) EAE-affected MSC-treated mice sacrificed at 6 h (6 h) and 24 h (24 h) after MSC treatment. a–d At both time points, the neocortex BBB-microvessels show traces of FITC-dextran leakage as a fluorescent halo in the surrounding parenchyma (a, c; arrows); in contrast, no trace of leakage and diffuse fluorescence is detectable in EAE-affected MSC-treated mice (b, d); note, in the latter, the preserved/restored continuity of the perivascular astrocyte endfeet (b, d; arrowheads) that is apparently lost in untreated EAE-affected mice (a, c; arrowheads). TOPRO-3 nuclear counterstaining. Scale bars: a–f 25 µm |
PMC9429671_Fig4_389849.jpg | What is the central feature of this picture? | The radiographs of tooth #35. a The preoperative radiograph, showed that tooth #35 had large periapical lesion and overfilling intracanal medication. b The postoperative radiograph, showed that a larger amount of iRoot SP extruded. c The 12-month observation showed that periapical lesions were smaller according to the X-ray results. d The 36-month recall observation showed that periapical lesions disappeared radiographically. e Radiography results of 60-month following-up showed periapical lesions were invisible on tooth #35 and functioned without any symptoms. The over-filling iRoot SP appealed no absorption radiographically |
PMC9429671_Fig4_389848.jpg | Describe the main subject of this image. | The radiographs of tooth #35. a The preoperative radiograph, showed that tooth #35 had large periapical lesion and overfilling intracanal medication. b The postoperative radiograph, showed that a larger amount of iRoot SP extruded. c The 12-month observation showed that periapical lesions were smaller according to the X-ray results. d The 36-month recall observation showed that periapical lesions disappeared radiographically. e Radiography results of 60-month following-up showed periapical lesions were invisible on tooth #35 and functioned without any symptoms. The over-filling iRoot SP appealed no absorption radiographically |
PMC9429671_Fig4_389845.jpg | What is the dominant medical problem in this image? | The radiographs of tooth #35. a The preoperative radiograph, showed that tooth #35 had large periapical lesion and overfilling intracanal medication. b The postoperative radiograph, showed that a larger amount of iRoot SP extruded. c The 12-month observation showed that periapical lesions were smaller according to the X-ray results. d The 36-month recall observation showed that periapical lesions disappeared radiographically. e Radiography results of 60-month following-up showed periapical lesions were invisible on tooth #35 and functioned without any symptoms. The over-filling iRoot SP appealed no absorption radiographically |
PMC9429671_Fig4_389847.jpg | What is the main focus of this visual representation? | The radiographs of tooth #35. a The preoperative radiograph, showed that tooth #35 had large periapical lesion and overfilling intracanal medication. b The postoperative radiograph, showed that a larger amount of iRoot SP extruded. c The 12-month observation showed that periapical lesions were smaller according to the X-ray results. d The 36-month recall observation showed that periapical lesions disappeared radiographically. e Radiography results of 60-month following-up showed periapical lesions were invisible on tooth #35 and functioned without any symptoms. The over-filling iRoot SP appealed no absorption radiographically |
PMC9429725_Fig2_389853.jpg | What is shown in this image? | A 70-year-old female patient with neck tumor for more than 4 months (A) Transverse image of ultrasonography, showing a heterogeneous hypoechoic nodule with circumscribed margin on the right lobe of thyroid. The arrows show the internal linear echogenic strands; (B) Longitudinal image of ultrasonography. The arrow shows a significant posterior echo enhancement; (C) Blood flow spectrum, showing rich blood flow signals; (D) Non-contrast CT. The arrow shows the CT of the right lobe of thyroid; (E) Venous phase CT, showing homogeneous mild enhancement in the right lobe nodule and the density of it is lower than that of the thyroid parenchyma; (F) Pathological results, showing mucosa-
associated lymphoid tissue lymphoma (MALT) (*400) |
PMC9429725_Fig2_389855.jpg | What does this image primarily show? | A 70-year-old female patient with neck tumor for more than 4 months (A) Transverse image of ultrasonography, showing a heterogeneous hypoechoic nodule with circumscribed margin on the right lobe of thyroid. The arrows show the internal linear echogenic strands; (B) Longitudinal image of ultrasonography. The arrow shows a significant posterior echo enhancement; (C) Blood flow spectrum, showing rich blood flow signals; (D) Non-contrast CT. The arrow shows the CT of the right lobe of thyroid; (E) Venous phase CT, showing homogeneous mild enhancement in the right lobe nodule and the density of it is lower than that of the thyroid parenchyma; (F) Pathological results, showing mucosa-
associated lymphoid tissue lymphoma (MALT) (*400) |
PMC9429725_Fig2_389852.jpg | What is being portrayed in this visual content? | A 70-year-old female patient with neck tumor for more than 4 months (A) Transverse image of ultrasonography, showing a heterogeneous hypoechoic nodule with circumscribed margin on the right lobe of thyroid. The arrows show the internal linear echogenic strands; (B) Longitudinal image of ultrasonography. The arrow shows a significant posterior echo enhancement; (C) Blood flow spectrum, showing rich blood flow signals; (D) Non-contrast CT. The arrow shows the CT of the right lobe of thyroid; (E) Venous phase CT, showing homogeneous mild enhancement in the right lobe nodule and the density of it is lower than that of the thyroid parenchyma; (F) Pathological results, showing mucosa-
associated lymphoid tissue lymphoma (MALT) (*400) |
PMC9429725_Fig2_389857.jpg | What is the core subject represented in this visual? | A 70-year-old female patient with neck tumor for more than 4 months (A) Transverse image of ultrasonography, showing a heterogeneous hypoechoic nodule with circumscribed margin on the right lobe of thyroid. The arrows show the internal linear echogenic strands; (B) Longitudinal image of ultrasonography. The arrow shows a significant posterior echo enhancement; (C) Blood flow spectrum, showing rich blood flow signals; (D) Non-contrast CT. The arrow shows the CT of the right lobe of thyroid; (E) Venous phase CT, showing homogeneous mild enhancement in the right lobe nodule and the density of it is lower than that of the thyroid parenchyma; (F) Pathological results, showing mucosa-
associated lymphoid tissue lymphoma (MALT) (*400) |
PMC9429725_Fig3_389860.jpg | What does this image primarily show? | A 50-year-old female patient with neck tumor for 3 months (A) Transverse image of ultrasonography, showing heterogeneous hypoechoic nodules on the left lobe of thyroid with irregular border; (B) Longitudinal image of ultrasonography. The arrows show the internal small calcification; (C) Blood flow spectrum, showing rich blood flow signals; (D) Non-contrast CT, showing low-density nodules on the left lobe of thyroid; (E) Venous phase CT, showing heterogeneous significant enhancement of nodules in the left lobe of thyroid; (F) Pathological results, showing papillary thyroid carcinoma (* 400) |
PMC9429725_Fig3_389863.jpg | What is the principal component of this image? | A 50-year-old female patient with neck tumor for 3 months (A) Transverse image of ultrasonography, showing heterogeneous hypoechoic nodules on the left lobe of thyroid with irregular border; (B) Longitudinal image of ultrasonography. The arrows show the internal small calcification; (C) Blood flow spectrum, showing rich blood flow signals; (D) Non-contrast CT, showing low-density nodules on the left lobe of thyroid; (E) Venous phase CT, showing heterogeneous significant enhancement of nodules in the left lobe of thyroid; (F) Pathological results, showing papillary thyroid carcinoma (* 400) |
PMC9429725_Fig3_389859.jpg | What is the principal component of this image? | A 50-year-old female patient with neck tumor for 3 months (A) Transverse image of ultrasonography, showing heterogeneous hypoechoic nodules on the left lobe of thyroid with irregular border; (B) Longitudinal image of ultrasonography. The arrows show the internal small calcification; (C) Blood flow spectrum, showing rich blood flow signals; (D) Non-contrast CT, showing low-density nodules on the left lobe of thyroid; (E) Venous phase CT, showing heterogeneous significant enhancement of nodules in the left lobe of thyroid; (F) Pathological results, showing papillary thyroid carcinoma (* 400) |
PMC9429725_Fig3_389862.jpg | What key item or scene is captured in this photo? | A 50-year-old female patient with neck tumor for 3 months (A) Transverse image of ultrasonography, showing heterogeneous hypoechoic nodules on the left lobe of thyroid with irregular border; (B) Longitudinal image of ultrasonography. The arrows show the internal small calcification; (C) Blood flow spectrum, showing rich blood flow signals; (D) Non-contrast CT, showing low-density nodules on the left lobe of thyroid; (E) Venous phase CT, showing heterogeneous significant enhancement of nodules in the left lobe of thyroid; (F) Pathological results, showing papillary thyroid carcinoma (* 400) |
PMC9429726_Fig1_389850.jpg | What is the principal component of this image? | Computed tomography pulmonary angiography shows extensive thrombus burden in segmental branches of pulmonary artery. a: thrombus (red arrow) in left lower pulmonary artery trunk; b: thrombi (red arrows) in posterior basal segment of both lower lobes |
PMC9429726_Fig1_389851.jpg | What object or scene is depicted here? | Computed tomography pulmonary angiography shows extensive thrombus burden in segmental branches of pulmonary artery. a: thrombus (red arrow) in left lower pulmonary artery trunk; b: thrombi (red arrows) in posterior basal segment of both lower lobes |
PMC9429726_Fig2_389865.jpg | What is the focal point of this photograph? | Thoracic spinal cord MRI. Sagittal T1-weighted MRI shows signal enhancement in the gray matter of the spinal cord (a); Axial T2-weighted MRI shows increased signal intensity in the gray matter segment of the spinal cord (b); Sagittal fat-suppressed sequence MRI shows enhanced signals of spinal cord swelling (c) |
PMC9429726_Fig2_389866.jpg | What is the dominant medical problem in this image? | Thoracic spinal cord MRI. Sagittal T1-weighted MRI shows signal enhancement in the gray matter of the spinal cord (a); Axial T2-weighted MRI shows increased signal intensity in the gray matter segment of the spinal cord (b); Sagittal fat-suppressed sequence MRI shows enhanced signals of spinal cord swelling (c) |
PMC9429726_Fig2_389864.jpg | What is the central feature of this picture? | Thoracic spinal cord MRI. Sagittal T1-weighted MRI shows signal enhancement in the gray matter of the spinal cord (a); Axial T2-weighted MRI shows increased signal intensity in the gray matter segment of the spinal cord (b); Sagittal fat-suppressed sequence MRI shows enhanced signals of spinal cord swelling (c) |
PMC9429763_Fig6_389877.jpg | What is the central feature of this picture? | Design, processing and architecture of the PCL/PLCL scaffold. A (a, b) The CT scanning images of the urethra; (d-f) The CT scanning images of the urethra filled with contrast reagent; (g) 2D slice (single layer) of the urethra design made using the WFIRM printing code program; where Red: UCs-laden hydrogel; Green: scaffold made of PCL/PLCL 50:50 blend; and Blue: SMCs-laden hydrogel. (h) The 3D rendering of the urethral design with porous scaffold and two hydrogel layers as seen using the WFIRM printing code program. B (a) PCL scaffold with columnar design; (b) PCL scaffold with spiral design; (c) PCL/PLCL (50:50) scaffold with columnar design; (d) PCL/PLCL (50:50) scaffold with spiral design; (e) native rabbit urethra. Scale bar: 2 mm (C) Stress testing of spiral and columnar scaffolds with PCL/PLCL blend (50:50). (D) UCs (labeled with green fluorescent dye) as seen in the hydrogel component of the bioprinted urethral construct after 1 day and 7 days of culture (a, c) and SMCs (labeled with red fluorescent dye) in the hydrogel component of the bioprinted urethral construct after 1 day of culture (b, d). Scale bar: 100 μm |
PMC9429763_Fig6_389873.jpg | What is the dominant medical problem in this image? | Design, processing and architecture of the PCL/PLCL scaffold. A (a, b) The CT scanning images of the urethra; (d-f) The CT scanning images of the urethra filled with contrast reagent; (g) 2D slice (single layer) of the urethra design made using the WFIRM printing code program; where Red: UCs-laden hydrogel; Green: scaffold made of PCL/PLCL 50:50 blend; and Blue: SMCs-laden hydrogel. (h) The 3D rendering of the urethral design with porous scaffold and two hydrogel layers as seen using the WFIRM printing code program. B (a) PCL scaffold with columnar design; (b) PCL scaffold with spiral design; (c) PCL/PLCL (50:50) scaffold with columnar design; (d) PCL/PLCL (50:50) scaffold with spiral design; (e) native rabbit urethra. Scale bar: 2 mm (C) Stress testing of spiral and columnar scaffolds with PCL/PLCL blend (50:50). (D) UCs (labeled with green fluorescent dye) as seen in the hydrogel component of the bioprinted urethral construct after 1 day and 7 days of culture (a, c) and SMCs (labeled with red fluorescent dye) in the hydrogel component of the bioprinted urethral construct after 1 day of culture (b, d). Scale bar: 100 μm |
PMC9429763_Fig6_389876.jpg | Describe the main subject of this image. | Design, processing and architecture of the PCL/PLCL scaffold. A (a, b) The CT scanning images of the urethra; (d-f) The CT scanning images of the urethra filled with contrast reagent; (g) 2D slice (single layer) of the urethra design made using the WFIRM printing code program; where Red: UCs-laden hydrogel; Green: scaffold made of PCL/PLCL 50:50 blend; and Blue: SMCs-laden hydrogel. (h) The 3D rendering of the urethral design with porous scaffold and two hydrogel layers as seen using the WFIRM printing code program. B (a) PCL scaffold with columnar design; (b) PCL scaffold with spiral design; (c) PCL/PLCL (50:50) scaffold with columnar design; (d) PCL/PLCL (50:50) scaffold with spiral design; (e) native rabbit urethra. Scale bar: 2 mm (C) Stress testing of spiral and columnar scaffolds with PCL/PLCL blend (50:50). (D) UCs (labeled with green fluorescent dye) as seen in the hydrogel component of the bioprinted urethral construct after 1 day and 7 days of culture (a, c) and SMCs (labeled with red fluorescent dye) in the hydrogel component of the bioprinted urethral construct after 1 day of culture (b, d). Scale bar: 100 μm |
PMC9429763_Fig6_389878.jpg | What's the most prominent thing you notice in this picture? | Design, processing and architecture of the PCL/PLCL scaffold. A (a, b) The CT scanning images of the urethra; (d-f) The CT scanning images of the urethra filled with contrast reagent; (g) 2D slice (single layer) of the urethra design made using the WFIRM printing code program; where Red: UCs-laden hydrogel; Green: scaffold made of PCL/PLCL 50:50 blend; and Blue: SMCs-laden hydrogel. (h) The 3D rendering of the urethral design with porous scaffold and two hydrogel layers as seen using the WFIRM printing code program. B (a) PCL scaffold with columnar design; (b) PCL scaffold with spiral design; (c) PCL/PLCL (50:50) scaffold with columnar design; (d) PCL/PLCL (50:50) scaffold with spiral design; (e) native rabbit urethra. Scale bar: 2 mm (C) Stress testing of spiral and columnar scaffolds with PCL/PLCL blend (50:50). (D) UCs (labeled with green fluorescent dye) as seen in the hydrogel component of the bioprinted urethral construct after 1 day and 7 days of culture (a, c) and SMCs (labeled with red fluorescent dye) in the hydrogel component of the bioprinted urethral construct after 1 day of culture (b, d). Scale bar: 100 μm |
PMC9429763_Fig6_389879.jpg | What does this image primarily show? | Design, processing and architecture of the PCL/PLCL scaffold. A (a, b) The CT scanning images of the urethra; (d-f) The CT scanning images of the urethra filled with contrast reagent; (g) 2D slice (single layer) of the urethra design made using the WFIRM printing code program; where Red: UCs-laden hydrogel; Green: scaffold made of PCL/PLCL 50:50 blend; and Blue: SMCs-laden hydrogel. (h) The 3D rendering of the urethral design with porous scaffold and two hydrogel layers as seen using the WFIRM printing code program. B (a) PCL scaffold with columnar design; (b) PCL scaffold with spiral design; (c) PCL/PLCL (50:50) scaffold with columnar design; (d) PCL/PLCL (50:50) scaffold with spiral design; (e) native rabbit urethra. Scale bar: 2 mm (C) Stress testing of spiral and columnar scaffolds with PCL/PLCL blend (50:50). (D) UCs (labeled with green fluorescent dye) as seen in the hydrogel component of the bioprinted urethral construct after 1 day and 7 days of culture (a, c) and SMCs (labeled with red fluorescent dye) in the hydrogel component of the bioprinted urethral construct after 1 day of culture (b, d). Scale bar: 100 μm |
PMC9429763_Fig6_389871.jpg | What is being portrayed in this visual content? | Design, processing and architecture of the PCL/PLCL scaffold. A (a, b) The CT scanning images of the urethra; (d-f) The CT scanning images of the urethra filled with contrast reagent; (g) 2D slice (single layer) of the urethra design made using the WFIRM printing code program; where Red: UCs-laden hydrogel; Green: scaffold made of PCL/PLCL 50:50 blend; and Blue: SMCs-laden hydrogel. (h) The 3D rendering of the urethral design with porous scaffold and two hydrogel layers as seen using the WFIRM printing code program. B (a) PCL scaffold with columnar design; (b) PCL scaffold with spiral design; (c) PCL/PLCL (50:50) scaffold with columnar design; (d) PCL/PLCL (50:50) scaffold with spiral design; (e) native rabbit urethra. Scale bar: 2 mm (C) Stress testing of spiral and columnar scaffolds with PCL/PLCL blend (50:50). (D) UCs (labeled with green fluorescent dye) as seen in the hydrogel component of the bioprinted urethral construct after 1 day and 7 days of culture (a, c) and SMCs (labeled with red fluorescent dye) in the hydrogel component of the bioprinted urethral construct after 1 day of culture (b, d). Scale bar: 100 μm |
PMC9429763_Fig6_389867.jpg | What is being portrayed in this visual content? | Design, processing and architecture of the PCL/PLCL scaffold. A (a, b) The CT scanning images of the urethra; (d-f) The CT scanning images of the urethra filled with contrast reagent; (g) 2D slice (single layer) of the urethra design made using the WFIRM printing code program; where Red: UCs-laden hydrogel; Green: scaffold made of PCL/PLCL 50:50 blend; and Blue: SMCs-laden hydrogel. (h) The 3D rendering of the urethral design with porous scaffold and two hydrogel layers as seen using the WFIRM printing code program. B (a) PCL scaffold with columnar design; (b) PCL scaffold with spiral design; (c) PCL/PLCL (50:50) scaffold with columnar design; (d) PCL/PLCL (50:50) scaffold with spiral design; (e) native rabbit urethra. Scale bar: 2 mm (C) Stress testing of spiral and columnar scaffolds with PCL/PLCL blend (50:50). (D) UCs (labeled with green fluorescent dye) as seen in the hydrogel component of the bioprinted urethral construct after 1 day and 7 days of culture (a, c) and SMCs (labeled with red fluorescent dye) in the hydrogel component of the bioprinted urethral construct after 1 day of culture (b, d). Scale bar: 100 μm |
PMC9429763_Fig6_389869.jpg | Describe the main subject of this image. | Design, processing and architecture of the PCL/PLCL scaffold. A (a, b) The CT scanning images of the urethra; (d-f) The CT scanning images of the urethra filled with contrast reagent; (g) 2D slice (single layer) of the urethra design made using the WFIRM printing code program; where Red: UCs-laden hydrogel; Green: scaffold made of PCL/PLCL 50:50 blend; and Blue: SMCs-laden hydrogel. (h) The 3D rendering of the urethral design with porous scaffold and two hydrogel layers as seen using the WFIRM printing code program. B (a) PCL scaffold with columnar design; (b) PCL scaffold with spiral design; (c) PCL/PLCL (50:50) scaffold with columnar design; (d) PCL/PLCL (50:50) scaffold with spiral design; (e) native rabbit urethra. Scale bar: 2 mm (C) Stress testing of spiral and columnar scaffolds with PCL/PLCL blend (50:50). (D) UCs (labeled with green fluorescent dye) as seen in the hydrogel component of the bioprinted urethral construct after 1 day and 7 days of culture (a, c) and SMCs (labeled with red fluorescent dye) in the hydrogel component of the bioprinted urethral construct after 1 day of culture (b, d). Scale bar: 100 μm |
PMC9429763_Fig6_389868.jpg | What is being portrayed in this visual content? | Design, processing and architecture of the PCL/PLCL scaffold. A (a, b) The CT scanning images of the urethra; (d-f) The CT scanning images of the urethra filled with contrast reagent; (g) 2D slice (single layer) of the urethra design made using the WFIRM printing code program; where Red: UCs-laden hydrogel; Green: scaffold made of PCL/PLCL 50:50 blend; and Blue: SMCs-laden hydrogel. (h) The 3D rendering of the urethral design with porous scaffold and two hydrogel layers as seen using the WFIRM printing code program. B (a) PCL scaffold with columnar design; (b) PCL scaffold with spiral design; (c) PCL/PLCL (50:50) scaffold with columnar design; (d) PCL/PLCL (50:50) scaffold with spiral design; (e) native rabbit urethra. Scale bar: 2 mm (C) Stress testing of spiral and columnar scaffolds with PCL/PLCL blend (50:50). (D) UCs (labeled with green fluorescent dye) as seen in the hydrogel component of the bioprinted urethral construct after 1 day and 7 days of culture (a, c) and SMCs (labeled with red fluorescent dye) in the hydrogel component of the bioprinted urethral construct after 1 day of culture (b, d). Scale bar: 100 μm |
PMC9429768_Fig2_389882.jpg | What is the main focus of this visual representation? | PVC with acute successful ablation near the atrioventricular node (AVN) (Patient No 6). a Twelve-lead ECG morphology of the QRS complex during SR and PVC. b CARTO3 mapping indicates an acute successful RFCA site near the AVN (A stands for antegrade technique and R stands for reversed C curve technique, yellow dot is where local electrogram has prominent his potentials in sinus beats). c Earliest V-QRS interval of 17 ms for bipolar recording during PVC, initial Q wave for unipolar recording, near field atrial electrogram (EGM) and an a/v amplitude ratio of 0.10 during SR (paper speed 100 mm/s), consistent with the recording from near the AVN with antegrade technique. d RFCA leads to elimination of the PVC after 4.87 s ablation delivery with reversed C curve technique. e and f Left and right anterior oblique fluoroscopic views indicate reversed C curve mapping technique. See the text for further details (paper speed 25 mm/s unless indicated). CS = coronary sinus; ABL = ablation catheter; MAP 1–2 = bipolar recording; MAP 1 = unipolar recording; Stim = stimulation. The same explanation as in Fig. 3 unless indicated |
PMC9429768_Fig2_389886.jpg | What is the focal point of this photograph? | PVC with acute successful ablation near the atrioventricular node (AVN) (Patient No 6). a Twelve-lead ECG morphology of the QRS complex during SR and PVC. b CARTO3 mapping indicates an acute successful RFCA site near the AVN (A stands for antegrade technique and R stands for reversed C curve technique, yellow dot is where local electrogram has prominent his potentials in sinus beats). c Earliest V-QRS interval of 17 ms for bipolar recording during PVC, initial Q wave for unipolar recording, near field atrial electrogram (EGM) and an a/v amplitude ratio of 0.10 during SR (paper speed 100 mm/s), consistent with the recording from near the AVN with antegrade technique. d RFCA leads to elimination of the PVC after 4.87 s ablation delivery with reversed C curve technique. e and f Left and right anterior oblique fluoroscopic views indicate reversed C curve mapping technique. See the text for further details (paper speed 25 mm/s unless indicated). CS = coronary sinus; ABL = ablation catheter; MAP 1–2 = bipolar recording; MAP 1 = unipolar recording; Stim = stimulation. The same explanation as in Fig. 3 unless indicated |
PMC9429783_Fig2_389887.jpg | Describe the main subject of this image. | A 66-year-old female patient with painless nonunion. A Post-operative X-ray B At three months postoperatively, bone absorption was observed around the plate. C At two years after the operation, she could perform daily activities without pain, despite nonunion |
PMC9429783_Fig2_389889.jpg | Can you identify the primary element in this image? | A 66-year-old female patient with painless nonunion. A Post-operative X-ray B At three months postoperatively, bone absorption was observed around the plate. C At two years after the operation, she could perform daily activities without pain, despite nonunion |
PMC9429789_fig1_389893.jpg | What stands out most in this visual? | (A) PET scan revealing involvement of the CD74-ROS1 NSCLC. Left upper scan revealing involvement of the left breast and mediastinal lymphadenopathy. Right upper scan revealing involvement in the left retropectoral region. Lower level scans revealing involvement of the left axillary lymph nodes at multiple levels. (B) Integrated genome viewer of the CHEK2 mutation S5fs∗54 (c.14_20delCGGATGT) of our patient case #1 with an allele frequency of 50.2% in ctDNA. (C) Clinical, genomic, and immunohistochemical characteristics of patient cases #1 and #2. #, number; AF, allele frequency; CT, computed tomography; ctDNA, circulating tumor DNA; IHC, immunohistochemistry; MB, megabase; MSI, microsatellite instability; NA, not available; PD-L1, programmed death-ligand 1; PET, positron emission tomography; TMB, tumor mutational burden. |
PMC9429808_F6_389895.jpg | What is the main focus of this visual representation? | Effect of JA priming on Spike /m2
(A), grains/spike (B), and weight of 100 grains [g (C)]; the ultrastructure of the leaf of two wheat cultivars Mai9 (ZM9, salt-sensitive) and Yang Mai25 (YM25, salt-tolerant) under salinity stress, CK [without priming under normal conditions in YM25 (D) and ZM9 (G)]; S1 [exposed to salinity without JA priming in YM25 (E) and ZM9 (H)]; JA priming+S1 [exposed to salinity with JA priming in YM25 (F) and ZM9 (I)]. |
PMC9429808_F6_389894.jpg | Can you identify the primary element in this image? | Effect of JA priming on Spike /m2
(A), grains/spike (B), and weight of 100 grains [g (C)]; the ultrastructure of the leaf of two wheat cultivars Mai9 (ZM9, salt-sensitive) and Yang Mai25 (YM25, salt-tolerant) under salinity stress, CK [without priming under normal conditions in YM25 (D) and ZM9 (G)]; S1 [exposed to salinity without JA priming in YM25 (E) and ZM9 (H)]; JA priming+S1 [exposed to salinity with JA priming in YM25 (F) and ZM9 (I)]. |
PMC9429808_F6_389897.jpg | What is the core subject represented in this visual? | Effect of JA priming on Spike /m2
(A), grains/spike (B), and weight of 100 grains [g (C)]; the ultrastructure of the leaf of two wheat cultivars Mai9 (ZM9, salt-sensitive) and Yang Mai25 (YM25, salt-tolerant) under salinity stress, CK [without priming under normal conditions in YM25 (D) and ZM9 (G)]; S1 [exposed to salinity without JA priming in YM25 (E) and ZM9 (H)]; JA priming+S1 [exposed to salinity with JA priming in YM25 (F) and ZM9 (I)]. |
PMC9429808_F6_389896.jpg | What key item or scene is captured in this photo? | Effect of JA priming on Spike /m2
(A), grains/spike (B), and weight of 100 grains [g (C)]; the ultrastructure of the leaf of two wheat cultivars Mai9 (ZM9, salt-sensitive) and Yang Mai25 (YM25, salt-tolerant) under salinity stress, CK [without priming under normal conditions in YM25 (D) and ZM9 (G)]; S1 [exposed to salinity without JA priming in YM25 (E) and ZM9 (H)]; JA priming+S1 [exposed to salinity with JA priming in YM25 (F) and ZM9 (I)]. |
PMC9429808_F6_389902.jpg | What is being portrayed in this visual content? | Effect of JA priming on Spike /m2
(A), grains/spike (B), and weight of 100 grains [g (C)]; the ultrastructure of the leaf of two wheat cultivars Mai9 (ZM9, salt-sensitive) and Yang Mai25 (YM25, salt-tolerant) under salinity stress, CK [without priming under normal conditions in YM25 (D) and ZM9 (G)]; S1 [exposed to salinity without JA priming in YM25 (E) and ZM9 (H)]; JA priming+S1 [exposed to salinity with JA priming in YM25 (F) and ZM9 (I)]. |
PMC9429827_F1_389905.jpg | Describe the main subject of this image. | CT image of an exophytic SPN located in the head of the pancreas. (A) Plain CT scan of the abdomen showed a tumor in the head of the pancreas, showing an exophytic growth. (B) On contrast-enhanced CT, irregular separation can be seen in the cystic part of the cyst, and fibrous component enhancement can be seen in the cyst fluid, showing a “floating cloud sign”. (C) CT images of patients with SPN rupture caused by trauma. |
PMC9429827_F1_389904.jpg | What is the main focus of this visual representation? | CT image of an exophytic SPN located in the head of the pancreas. (A) Plain CT scan of the abdomen showed a tumor in the head of the pancreas, showing an exophytic growth. (B) On contrast-enhanced CT, irregular separation can be seen in the cystic part of the cyst, and fibrous component enhancement can be seen in the cyst fluid, showing a “floating cloud sign”. (C) CT images of patients with SPN rupture caused by trauma. |
PMC9429827_F1_389903.jpg | What does this image primarily show? | CT image of an exophytic SPN located in the head of the pancreas. (A) Plain CT scan of the abdomen showed a tumor in the head of the pancreas, showing an exophytic growth. (B) On contrast-enhanced CT, irregular separation can be seen in the cystic part of the cyst, and fibrous component enhancement can be seen in the cyst fluid, showing a “floating cloud sign”. (C) CT images of patients with SPN rupture caused by trauma. |
PMC9429888_fig4_389907.jpg | What key item or scene is captured in this photo? | SEM of normal BSF (top and middle) and procyclic form (PCF, bottom) trypanosomes at different voltages. BSF images were acquired at an oblique angle; the PCF image was acquired vertically. |
PMC9429888_fig4_389908.jpg | What's the most prominent thing you notice in this picture? | SEM of normal BSF (top and middle) and procyclic form (PCF, bottom) trypanosomes at different voltages. BSF images were acquired at an oblique angle; the PCF image was acquired vertically. |
PMC9429888_fig4_389906.jpg | What is the dominant medical problem in this image? | SEM of normal BSF (top and middle) and procyclic form (PCF, bottom) trypanosomes at different voltages. BSF images were acquired at an oblique angle; the PCF image was acquired vertically. |
PMC9429927_fig7_389919.jpg | What does this image primarily show? | The cell walls of all deletion strain cells are intact. Transmission electron microscopy of KN99 cells at a magnification of ×3,000 (A), KN99 cells at ×25,000 (B), cft1Δ cells at ×3,000 (C), cft1Δ cells at ×25,000 (D), erg4Δ cells at ×3,000 (E), erg4Δ cells at ×25,000 (F), myo1Δ cells at ×3,000 (G), myo1Δ cells at ×25,000 (H), ysp2Δ cells at ×3,000 (I), and ysp2Δ cells at ×25,000 (J). The boxed areas in the ×25,000 magnification images are shown enlarged to the right of the original images. The scale bars show 2 μm at ×3,000 magnification and 500 nm at ×25,000 magnification. PM, plasma membrane; CW, cell wall; C, capsular material. Cells were grown overnight in YPD at 30°C prior to preparation for imaging. Sections were stained with uranyl acetate and lead acetate. |
PMC9429927_fig7_389911.jpg | What is the main focus of this visual representation? | The cell walls of all deletion strain cells are intact. Transmission electron microscopy of KN99 cells at a magnification of ×3,000 (A), KN99 cells at ×25,000 (B), cft1Δ cells at ×3,000 (C), cft1Δ cells at ×25,000 (D), erg4Δ cells at ×3,000 (E), erg4Δ cells at ×25,000 (F), myo1Δ cells at ×3,000 (G), myo1Δ cells at ×25,000 (H), ysp2Δ cells at ×3,000 (I), and ysp2Δ cells at ×25,000 (J). The boxed areas in the ×25,000 magnification images are shown enlarged to the right of the original images. The scale bars show 2 μm at ×3,000 magnification and 500 nm at ×25,000 magnification. PM, plasma membrane; CW, cell wall; C, capsular material. Cells were grown overnight in YPD at 30°C prior to preparation for imaging. Sections were stained with uranyl acetate and lead acetate. |
PMC9429927_fig7_389916.jpg | What is the dominant medical problem in this image? | The cell walls of all deletion strain cells are intact. Transmission electron microscopy of KN99 cells at a magnification of ×3,000 (A), KN99 cells at ×25,000 (B), cft1Δ cells at ×3,000 (C), cft1Δ cells at ×25,000 (D), erg4Δ cells at ×3,000 (E), erg4Δ cells at ×25,000 (F), myo1Δ cells at ×3,000 (G), myo1Δ cells at ×25,000 (H), ysp2Δ cells at ×3,000 (I), and ysp2Δ cells at ×25,000 (J). The boxed areas in the ×25,000 magnification images are shown enlarged to the right of the original images. The scale bars show 2 μm at ×3,000 magnification and 500 nm at ×25,000 magnification. PM, plasma membrane; CW, cell wall; C, capsular material. Cells were grown overnight in YPD at 30°C prior to preparation for imaging. Sections were stained with uranyl acetate and lead acetate. |
PMC9429927_fig7_389910.jpg | Can you identify the primary element in this image? | The cell walls of all deletion strain cells are intact. Transmission electron microscopy of KN99 cells at a magnification of ×3,000 (A), KN99 cells at ×25,000 (B), cft1Δ cells at ×3,000 (C), cft1Δ cells at ×25,000 (D), erg4Δ cells at ×3,000 (E), erg4Δ cells at ×25,000 (F), myo1Δ cells at ×3,000 (G), myo1Δ cells at ×25,000 (H), ysp2Δ cells at ×3,000 (I), and ysp2Δ cells at ×25,000 (J). The boxed areas in the ×25,000 magnification images are shown enlarged to the right of the original images. The scale bars show 2 μm at ×3,000 magnification and 500 nm at ×25,000 magnification. PM, plasma membrane; CW, cell wall; C, capsular material. Cells were grown overnight in YPD at 30°C prior to preparation for imaging. Sections were stained with uranyl acetate and lead acetate. |
PMC9429927_fig7_389921.jpg | What is the principal component of this image? | The cell walls of all deletion strain cells are intact. Transmission electron microscopy of KN99 cells at a magnification of ×3,000 (A), KN99 cells at ×25,000 (B), cft1Δ cells at ×3,000 (C), cft1Δ cells at ×25,000 (D), erg4Δ cells at ×3,000 (E), erg4Δ cells at ×25,000 (F), myo1Δ cells at ×3,000 (G), myo1Δ cells at ×25,000 (H), ysp2Δ cells at ×3,000 (I), and ysp2Δ cells at ×25,000 (J). The boxed areas in the ×25,000 magnification images are shown enlarged to the right of the original images. The scale bars show 2 μm at ×3,000 magnification and 500 nm at ×25,000 magnification. PM, plasma membrane; CW, cell wall; C, capsular material. Cells were grown overnight in YPD at 30°C prior to preparation for imaging. Sections were stained with uranyl acetate and lead acetate. |
PMC9429927_fig7_389918.jpg | What is the focal point of this photograph? | The cell walls of all deletion strain cells are intact. Transmission electron microscopy of KN99 cells at a magnification of ×3,000 (A), KN99 cells at ×25,000 (B), cft1Δ cells at ×3,000 (C), cft1Δ cells at ×25,000 (D), erg4Δ cells at ×3,000 (E), erg4Δ cells at ×25,000 (F), myo1Δ cells at ×3,000 (G), myo1Δ cells at ×25,000 (H), ysp2Δ cells at ×3,000 (I), and ysp2Δ cells at ×25,000 (J). The boxed areas in the ×25,000 magnification images are shown enlarged to the right of the original images. The scale bars show 2 μm at ×3,000 magnification and 500 nm at ×25,000 magnification. PM, plasma membrane; CW, cell wall; C, capsular material. Cells were grown overnight in YPD at 30°C prior to preparation for imaging. Sections were stained with uranyl acetate and lead acetate. |
PMC9429927_fig7_389913.jpg | What object or scene is depicted here? | The cell walls of all deletion strain cells are intact. Transmission electron microscopy of KN99 cells at a magnification of ×3,000 (A), KN99 cells at ×25,000 (B), cft1Δ cells at ×3,000 (C), cft1Δ cells at ×25,000 (D), erg4Δ cells at ×3,000 (E), erg4Δ cells at ×25,000 (F), myo1Δ cells at ×3,000 (G), myo1Δ cells at ×25,000 (H), ysp2Δ cells at ×3,000 (I), and ysp2Δ cells at ×25,000 (J). The boxed areas in the ×25,000 magnification images are shown enlarged to the right of the original images. The scale bars show 2 μm at ×3,000 magnification and 500 nm at ×25,000 magnification. PM, plasma membrane; CW, cell wall; C, capsular material. Cells were grown overnight in YPD at 30°C prior to preparation for imaging. Sections were stained with uranyl acetate and lead acetate. |
PMC9429927_fig7_389920.jpg | Can you identify the primary element in this image? | The cell walls of all deletion strain cells are intact. Transmission electron microscopy of KN99 cells at a magnification of ×3,000 (A), KN99 cells at ×25,000 (B), cft1Δ cells at ×3,000 (C), cft1Δ cells at ×25,000 (D), erg4Δ cells at ×3,000 (E), erg4Δ cells at ×25,000 (F), myo1Δ cells at ×3,000 (G), myo1Δ cells at ×25,000 (H), ysp2Δ cells at ×3,000 (I), and ysp2Δ cells at ×25,000 (J). The boxed areas in the ×25,000 magnification images are shown enlarged to the right of the original images. The scale bars show 2 μm at ×3,000 magnification and 500 nm at ×25,000 magnification. PM, plasma membrane; CW, cell wall; C, capsular material. Cells were grown overnight in YPD at 30°C prior to preparation for imaging. Sections were stained with uranyl acetate and lead acetate. |
PMC9429927_fig7_389912.jpg | What is the main focus of this visual representation? | The cell walls of all deletion strain cells are intact. Transmission electron microscopy of KN99 cells at a magnification of ×3,000 (A), KN99 cells at ×25,000 (B), cft1Δ cells at ×3,000 (C), cft1Δ cells at ×25,000 (D), erg4Δ cells at ×3,000 (E), erg4Δ cells at ×25,000 (F), myo1Δ cells at ×3,000 (G), myo1Δ cells at ×25,000 (H), ysp2Δ cells at ×3,000 (I), and ysp2Δ cells at ×25,000 (J). The boxed areas in the ×25,000 magnification images are shown enlarged to the right of the original images. The scale bars show 2 μm at ×3,000 magnification and 500 nm at ×25,000 magnification. PM, plasma membrane; CW, cell wall; C, capsular material. Cells were grown overnight in YPD at 30°C prior to preparation for imaging. Sections were stained with uranyl acetate and lead acetate. |
PMC9429927_fig7_389914.jpg | What does this image primarily show? | The cell walls of all deletion strain cells are intact. Transmission electron microscopy of KN99 cells at a magnification of ×3,000 (A), KN99 cells at ×25,000 (B), cft1Δ cells at ×3,000 (C), cft1Δ cells at ×25,000 (D), erg4Δ cells at ×3,000 (E), erg4Δ cells at ×25,000 (F), myo1Δ cells at ×3,000 (G), myo1Δ cells at ×25,000 (H), ysp2Δ cells at ×3,000 (I), and ysp2Δ cells at ×25,000 (J). The boxed areas in the ×25,000 magnification images are shown enlarged to the right of the original images. The scale bars show 2 μm at ×3,000 magnification and 500 nm at ×25,000 magnification. PM, plasma membrane; CW, cell wall; C, capsular material. Cells were grown overnight in YPD at 30°C prior to preparation for imaging. Sections were stained with uranyl acetate and lead acetate. |
PMC9429927_fig7_389915.jpg | What is the focal point of this photograph? | The cell walls of all deletion strain cells are intact. Transmission electron microscopy of KN99 cells at a magnification of ×3,000 (A), KN99 cells at ×25,000 (B), cft1Δ cells at ×3,000 (C), cft1Δ cells at ×25,000 (D), erg4Δ cells at ×3,000 (E), erg4Δ cells at ×25,000 (F), myo1Δ cells at ×3,000 (G), myo1Δ cells at ×25,000 (H), ysp2Δ cells at ×3,000 (I), and ysp2Δ cells at ×25,000 (J). The boxed areas in the ×25,000 magnification images are shown enlarged to the right of the original images. The scale bars show 2 μm at ×3,000 magnification and 500 nm at ×25,000 magnification. PM, plasma membrane; CW, cell wall; C, capsular material. Cells were grown overnight in YPD at 30°C prior to preparation for imaging. Sections were stained with uranyl acetate and lead acetate. |
PMC9429927_fig7_389909.jpg | What key item or scene is captured in this photo? | The cell walls of all deletion strain cells are intact. Transmission electron microscopy of KN99 cells at a magnification of ×3,000 (A), KN99 cells at ×25,000 (B), cft1Δ cells at ×3,000 (C), cft1Δ cells at ×25,000 (D), erg4Δ cells at ×3,000 (E), erg4Δ cells at ×25,000 (F), myo1Δ cells at ×3,000 (G), myo1Δ cells at ×25,000 (H), ysp2Δ cells at ×3,000 (I), and ysp2Δ cells at ×25,000 (J). The boxed areas in the ×25,000 magnification images are shown enlarged to the right of the original images. The scale bars show 2 μm at ×3,000 magnification and 500 nm at ×25,000 magnification. PM, plasma membrane; CW, cell wall; C, capsular material. Cells were grown overnight in YPD at 30°C prior to preparation for imaging. Sections were stained with uranyl acetate and lead acetate. |
PMC9429943_fig2_389942.jpg | What does this image primarily show? | On resulting electron microscopy (EM) samples: hydrogen peroxide concentration, cell wall removal, and a rare case of spill over staining. (A) High concentration of hydrogen peroxide during DAB staining compromises sample structure and obscures fine detail. Yeast cells expressing PCOX4-Cox4-V5-APEX2 (labeling mitochondria) were chemically fixed, stained by DAB, and then processed for transmission EM. Correlation between staining intensity and hydrogen peroxide concentration was visible in both light microscopy and EM. Fractures along stained membrane (yellow arrowheads) were frequent in samples treated with higher concentration of hydrogen peroxide. White arrows, mitochondria with acceptable structural preservation. (B) Cell wall removal is not necessary for the use of APEX2 in EM. Yeast cells were processed for EM in the same procedure, except that in one group enzymatic digestion of cells was performed (after chemical fixation, before potassium permanganate treatment). The inclusion of this step did not bring noticeable improvement. Arrows, organelles stained by DAB. (C) In rare cases, DAB staining may occur in unintended locations. Erg6-V5-APEX2 was the only exception among all the chimeras we tested. Erg6 is supposedly a lipid droplet protein. In EM, staining at the cell periphery was also present. Arrows, DAB staining in cell periphery. White scale bar in EM images, 1 um. Yellow scale bar in DIC images, 5 um. |
PMC9429943_fig2_389937.jpg | What is shown in this image? | On resulting electron microscopy (EM) samples: hydrogen peroxide concentration, cell wall removal, and a rare case of spill over staining. (A) High concentration of hydrogen peroxide during DAB staining compromises sample structure and obscures fine detail. Yeast cells expressing PCOX4-Cox4-V5-APEX2 (labeling mitochondria) were chemically fixed, stained by DAB, and then processed for transmission EM. Correlation between staining intensity and hydrogen peroxide concentration was visible in both light microscopy and EM. Fractures along stained membrane (yellow arrowheads) were frequent in samples treated with higher concentration of hydrogen peroxide. White arrows, mitochondria with acceptable structural preservation. (B) Cell wall removal is not necessary for the use of APEX2 in EM. Yeast cells were processed for EM in the same procedure, except that in one group enzymatic digestion of cells was performed (after chemical fixation, before potassium permanganate treatment). The inclusion of this step did not bring noticeable improvement. Arrows, organelles stained by DAB. (C) In rare cases, DAB staining may occur in unintended locations. Erg6-V5-APEX2 was the only exception among all the chimeras we tested. Erg6 is supposedly a lipid droplet protein. In EM, staining at the cell periphery was also present. Arrows, DAB staining in cell periphery. White scale bar in EM images, 1 um. Yellow scale bar in DIC images, 5 um. |
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