image
stringlengths 20
66
| question
stringclasses 16
values | answer
stringlengths 3
10.7k
|
|---|---|---|
PMC544194_F4_937.jpg | What is the main focus of this visual representation? | Example of micro CT identification of mineral composition. Visually, there appears to be only one mineral contained in the stone in the left panel and three different minerals that comprise the stone in the right panel. Micro CT attenuation identified the left stone as pure COM, and the right stone as a mixture of hydroxyapatite (bright white, highest attenuation), COM (gray) and uric acid (close to black). Speckled nature of colors – particularly apparent in image at left – is due to image noise as a result of increased magnification. |
PMC544194_F5_936.jpg | What is the central feature of this picture? | Imaging and software power of micro CT. Pure COM stone viewed by conventional photography (A) and micro CT (B-D). B shows a typical micro CT image slice, C a 3-D reconstruction of the stone surface, and D a wedge cut that displays the internal 3-D morphology of the stone. |
PMC544194_F5_933.jpg | What is the central feature of this picture? | Imaging and software power of micro CT. Pure COM stone viewed by conventional photography (A) and micro CT (B-D). B shows a typical micro CT image slice, C a 3-D reconstruction of the stone surface, and D a wedge cut that displays the internal 3-D morphology of the stone. |
PMC544194_F5_935.jpg | What is the focal point of this photograph? | Imaging and software power of micro CT. Pure COM stone viewed by conventional photography (A) and micro CT (B-D). B shows a typical micro CT image slice, C a 3-D reconstruction of the stone surface, and D a wedge cut that displays the internal 3-D morphology of the stone. |
PMC544401_F4_956.jpg | Can you identify the primary element in this image? | Live imaging H2B-EGFP in postimplantation mouse embryos. (a) Lateral view of the embryonic region of an E7.5 embryo (anterior to the left) with box depicting the region imaged in b and double-headed arrow depicting the x-y layering of the z-stack. (b-d) single optical x-y sections of fluorescence overlayed on bright-field images acquired at the same focal plane. Each panel is 60 μm apart from the preceding panel. These panels comprise x-y images in the z-stack depicted in panel a. The different layers of this stage of embryo including the epiblast, mesoderm, visceral endoderm and node can be distinguished on the basis of both position and nuclear morphology. (e-h) projection of a rendered z-stack of (x-y) sections (n = 90) of the dark-field component of the sections taken in the series schematized in a and of the raw data shown in b. (e) 0° rotation, (f) 60° rotation, (g) 120° rotation, and (h) 180° rotation views. (i) low-magnification frontal view of an E11 embryo that has had a transverse cut made to remove the head. The box depicts the region (at the ventral hindbrain and 1st branchial pouch) subject to laser scanning confocal imaging, with the double-headed arrow depicting the x-y layering of the acquired z-stack. (j) rendered (z-) stack of sections (n = 200, i.e. 400 μm depth) taken through the boxed region. (k) rendered stack of top 50 x-y sections (100 μm depth) taken from the region imaged around the notochord (comprising axial mesoderm and mesenchyme cells). (l) rendered stack of top 50 sections (100 μm depth) taken around the branchial pouch region (comprising endoderm and mesenchyme cells). The sections used to generate the rendered stacks in panels k and l were electronically magnified. Pink arrowheads, mitotic nuclei; yellow arrowheads, pycnotic nuclei; ect, ectoderm, en, endoderm, hf, headfold, mes, mesoderm, noto, notochord. |
PMC544401_F4_960.jpg | What is the focal point of this photograph? | Live imaging H2B-EGFP in postimplantation mouse embryos. (a) Lateral view of the embryonic region of an E7.5 embryo (anterior to the left) with box depicting the region imaged in b and double-headed arrow depicting the x-y layering of the z-stack. (b-d) single optical x-y sections of fluorescence overlayed on bright-field images acquired at the same focal plane. Each panel is 60 μm apart from the preceding panel. These panels comprise x-y images in the z-stack depicted in panel a. The different layers of this stage of embryo including the epiblast, mesoderm, visceral endoderm and node can be distinguished on the basis of both position and nuclear morphology. (e-h) projection of a rendered z-stack of (x-y) sections (n = 90) of the dark-field component of the sections taken in the series schematized in a and of the raw data shown in b. (e) 0° rotation, (f) 60° rotation, (g) 120° rotation, and (h) 180° rotation views. (i) low-magnification frontal view of an E11 embryo that has had a transverse cut made to remove the head. The box depicts the region (at the ventral hindbrain and 1st branchial pouch) subject to laser scanning confocal imaging, with the double-headed arrow depicting the x-y layering of the acquired z-stack. (j) rendered (z-) stack of sections (n = 200, i.e. 400 μm depth) taken through the boxed region. (k) rendered stack of top 50 x-y sections (100 μm depth) taken from the region imaged around the notochord (comprising axial mesoderm and mesenchyme cells). (l) rendered stack of top 50 sections (100 μm depth) taken around the branchial pouch region (comprising endoderm and mesenchyme cells). The sections used to generate the rendered stacks in panels k and l were electronically magnified. Pink arrowheads, mitotic nuclei; yellow arrowheads, pycnotic nuclei; ect, ectoderm, en, endoderm, hf, headfold, mes, mesoderm, noto, notochord. |
PMC544401_F4_957.jpg | What is the main focus of this visual representation? | Live imaging H2B-EGFP in postimplantation mouse embryos. (a) Lateral view of the embryonic region of an E7.5 embryo (anterior to the left) with box depicting the region imaged in b and double-headed arrow depicting the x-y layering of the z-stack. (b-d) single optical x-y sections of fluorescence overlayed on bright-field images acquired at the same focal plane. Each panel is 60 μm apart from the preceding panel. These panels comprise x-y images in the z-stack depicted in panel a. The different layers of this stage of embryo including the epiblast, mesoderm, visceral endoderm and node can be distinguished on the basis of both position and nuclear morphology. (e-h) projection of a rendered z-stack of (x-y) sections (n = 90) of the dark-field component of the sections taken in the series schematized in a and of the raw data shown in b. (e) 0° rotation, (f) 60° rotation, (g) 120° rotation, and (h) 180° rotation views. (i) low-magnification frontal view of an E11 embryo that has had a transverse cut made to remove the head. The box depicts the region (at the ventral hindbrain and 1st branchial pouch) subject to laser scanning confocal imaging, with the double-headed arrow depicting the x-y layering of the acquired z-stack. (j) rendered (z-) stack of sections (n = 200, i.e. 400 μm depth) taken through the boxed region. (k) rendered stack of top 50 x-y sections (100 μm depth) taken from the region imaged around the notochord (comprising axial mesoderm and mesenchyme cells). (l) rendered stack of top 50 sections (100 μm depth) taken around the branchial pouch region (comprising endoderm and mesenchyme cells). The sections used to generate the rendered stacks in panels k and l were electronically magnified. Pink arrowheads, mitotic nuclei; yellow arrowheads, pycnotic nuclei; ect, ectoderm, en, endoderm, hf, headfold, mes, mesoderm, noto, notochord. |
PMC544401_F4_955.jpg | What is the central feature of this picture? | Live imaging H2B-EGFP in postimplantation mouse embryos. (a) Lateral view of the embryonic region of an E7.5 embryo (anterior to the left) with box depicting the region imaged in b and double-headed arrow depicting the x-y layering of the z-stack. (b-d) single optical x-y sections of fluorescence overlayed on bright-field images acquired at the same focal plane. Each panel is 60 μm apart from the preceding panel. These panels comprise x-y images in the z-stack depicted in panel a. The different layers of this stage of embryo including the epiblast, mesoderm, visceral endoderm and node can be distinguished on the basis of both position and nuclear morphology. (e-h) projection of a rendered z-stack of (x-y) sections (n = 90) of the dark-field component of the sections taken in the series schematized in a and of the raw data shown in b. (e) 0° rotation, (f) 60° rotation, (g) 120° rotation, and (h) 180° rotation views. (i) low-magnification frontal view of an E11 embryo that has had a transverse cut made to remove the head. The box depicts the region (at the ventral hindbrain and 1st branchial pouch) subject to laser scanning confocal imaging, with the double-headed arrow depicting the x-y layering of the acquired z-stack. (j) rendered (z-) stack of sections (n = 200, i.e. 400 μm depth) taken through the boxed region. (k) rendered stack of top 50 x-y sections (100 μm depth) taken from the region imaged around the notochord (comprising axial mesoderm and mesenchyme cells). (l) rendered stack of top 50 sections (100 μm depth) taken around the branchial pouch region (comprising endoderm and mesenchyme cells). The sections used to generate the rendered stacks in panels k and l were electronically magnified. Pink arrowheads, mitotic nuclei; yellow arrowheads, pycnotic nuclei; ect, ectoderm, en, endoderm, hf, headfold, mes, mesoderm, noto, notochord. |
PMC544401_F4_961.jpg | Describe the main subject of this image. | Live imaging H2B-EGFP in postimplantation mouse embryos. (a) Lateral view of the embryonic region of an E7.5 embryo (anterior to the left) with box depicting the region imaged in b and double-headed arrow depicting the x-y layering of the z-stack. (b-d) single optical x-y sections of fluorescence overlayed on bright-field images acquired at the same focal plane. Each panel is 60 μm apart from the preceding panel. These panels comprise x-y images in the z-stack depicted in panel a. The different layers of this stage of embryo including the epiblast, mesoderm, visceral endoderm and node can be distinguished on the basis of both position and nuclear morphology. (e-h) projection of a rendered z-stack of (x-y) sections (n = 90) of the dark-field component of the sections taken in the series schematized in a and of the raw data shown in b. (e) 0° rotation, (f) 60° rotation, (g) 120° rotation, and (h) 180° rotation views. (i) low-magnification frontal view of an E11 embryo that has had a transverse cut made to remove the head. The box depicts the region (at the ventral hindbrain and 1st branchial pouch) subject to laser scanning confocal imaging, with the double-headed arrow depicting the x-y layering of the acquired z-stack. (j) rendered (z-) stack of sections (n = 200, i.e. 400 μm depth) taken through the boxed region. (k) rendered stack of top 50 x-y sections (100 μm depth) taken from the region imaged around the notochord (comprising axial mesoderm and mesenchyme cells). (l) rendered stack of top 50 sections (100 μm depth) taken around the branchial pouch region (comprising endoderm and mesenchyme cells). The sections used to generate the rendered stacks in panels k and l were electronically magnified. Pink arrowheads, mitotic nuclei; yellow arrowheads, pycnotic nuclei; ect, ectoderm, en, endoderm, hf, headfold, mes, mesoderm, noto, notochord. |
PMC544401_F4_963.jpg | What is the principal component of this image? | Live imaging H2B-EGFP in postimplantation mouse embryos. (a) Lateral view of the embryonic region of an E7.5 embryo (anterior to the left) with box depicting the region imaged in b and double-headed arrow depicting the x-y layering of the z-stack. (b-d) single optical x-y sections of fluorescence overlayed on bright-field images acquired at the same focal plane. Each panel is 60 μm apart from the preceding panel. These panels comprise x-y images in the z-stack depicted in panel a. The different layers of this stage of embryo including the epiblast, mesoderm, visceral endoderm and node can be distinguished on the basis of both position and nuclear morphology. (e-h) projection of a rendered z-stack of (x-y) sections (n = 90) of the dark-field component of the sections taken in the series schematized in a and of the raw data shown in b. (e) 0° rotation, (f) 60° rotation, (g) 120° rotation, and (h) 180° rotation views. (i) low-magnification frontal view of an E11 embryo that has had a transverse cut made to remove the head. The box depicts the region (at the ventral hindbrain and 1st branchial pouch) subject to laser scanning confocal imaging, with the double-headed arrow depicting the x-y layering of the acquired z-stack. (j) rendered (z-) stack of sections (n = 200, i.e. 400 μm depth) taken through the boxed region. (k) rendered stack of top 50 x-y sections (100 μm depth) taken from the region imaged around the notochord (comprising axial mesoderm and mesenchyme cells). (l) rendered stack of top 50 sections (100 μm depth) taken around the branchial pouch region (comprising endoderm and mesenchyme cells). The sections used to generate the rendered stacks in panels k and l were electronically magnified. Pink arrowheads, mitotic nuclei; yellow arrowheads, pycnotic nuclei; ect, ectoderm, en, endoderm, hf, headfold, mes, mesoderm, noto, notochord. |
PMC544401_F4_962.jpg | What is shown in this image? | Live imaging H2B-EGFP in postimplantation mouse embryos. (a) Lateral view of the embryonic region of an E7.5 embryo (anterior to the left) with box depicting the region imaged in b and double-headed arrow depicting the x-y layering of the z-stack. (b-d) single optical x-y sections of fluorescence overlayed on bright-field images acquired at the same focal plane. Each panel is 60 μm apart from the preceding panel. These panels comprise x-y images in the z-stack depicted in panel a. The different layers of this stage of embryo including the epiblast, mesoderm, visceral endoderm and node can be distinguished on the basis of both position and nuclear morphology. (e-h) projection of a rendered z-stack of (x-y) sections (n = 90) of the dark-field component of the sections taken in the series schematized in a and of the raw data shown in b. (e) 0° rotation, (f) 60° rotation, (g) 120° rotation, and (h) 180° rotation views. (i) low-magnification frontal view of an E11 embryo that has had a transverse cut made to remove the head. The box depicts the region (at the ventral hindbrain and 1st branchial pouch) subject to laser scanning confocal imaging, with the double-headed arrow depicting the x-y layering of the acquired z-stack. (j) rendered (z-) stack of sections (n = 200, i.e. 400 μm depth) taken through the boxed region. (k) rendered stack of top 50 x-y sections (100 μm depth) taken from the region imaged around the notochord (comprising axial mesoderm and mesenchyme cells). (l) rendered stack of top 50 sections (100 μm depth) taken around the branchial pouch region (comprising endoderm and mesenchyme cells). The sections used to generate the rendered stacks in panels k and l were electronically magnified. Pink arrowheads, mitotic nuclei; yellow arrowheads, pycnotic nuclei; ect, ectoderm, en, endoderm, hf, headfold, mes, mesoderm, noto, notochord. |
PMC544401_F4_953.jpg | What object or scene is depicted here? | Live imaging H2B-EGFP in postimplantation mouse embryos. (a) Lateral view of the embryonic region of an E7.5 embryo (anterior to the left) with box depicting the region imaged in b and double-headed arrow depicting the x-y layering of the z-stack. (b-d) single optical x-y sections of fluorescence overlayed on bright-field images acquired at the same focal plane. Each panel is 60 μm apart from the preceding panel. These panels comprise x-y images in the z-stack depicted in panel a. The different layers of this stage of embryo including the epiblast, mesoderm, visceral endoderm and node can be distinguished on the basis of both position and nuclear morphology. (e-h) projection of a rendered z-stack of (x-y) sections (n = 90) of the dark-field component of the sections taken in the series schematized in a and of the raw data shown in b. (e) 0° rotation, (f) 60° rotation, (g) 120° rotation, and (h) 180° rotation views. (i) low-magnification frontal view of an E11 embryo that has had a transverse cut made to remove the head. The box depicts the region (at the ventral hindbrain and 1st branchial pouch) subject to laser scanning confocal imaging, with the double-headed arrow depicting the x-y layering of the acquired z-stack. (j) rendered (z-) stack of sections (n = 200, i.e. 400 μm depth) taken through the boxed region. (k) rendered stack of top 50 x-y sections (100 μm depth) taken from the region imaged around the notochord (comprising axial mesoderm and mesenchyme cells). (l) rendered stack of top 50 sections (100 μm depth) taken around the branchial pouch region (comprising endoderm and mesenchyme cells). The sections used to generate the rendered stacks in panels k and l were electronically magnified. Pink arrowheads, mitotic nuclei; yellow arrowheads, pycnotic nuclei; ect, ectoderm, en, endoderm, hf, headfold, mes, mesoderm, noto, notochord. |
PMC544401_F4_958.jpg | What is the dominant medical problem in this image? | Live imaging H2B-EGFP in postimplantation mouse embryos. (a) Lateral view of the embryonic region of an E7.5 embryo (anterior to the left) with box depicting the region imaged in b and double-headed arrow depicting the x-y layering of the z-stack. (b-d) single optical x-y sections of fluorescence overlayed on bright-field images acquired at the same focal plane. Each panel is 60 μm apart from the preceding panel. These panels comprise x-y images in the z-stack depicted in panel a. The different layers of this stage of embryo including the epiblast, mesoderm, visceral endoderm and node can be distinguished on the basis of both position and nuclear morphology. (e-h) projection of a rendered z-stack of (x-y) sections (n = 90) of the dark-field component of the sections taken in the series schematized in a and of the raw data shown in b. (e) 0° rotation, (f) 60° rotation, (g) 120° rotation, and (h) 180° rotation views. (i) low-magnification frontal view of an E11 embryo that has had a transverse cut made to remove the head. The box depicts the region (at the ventral hindbrain and 1st branchial pouch) subject to laser scanning confocal imaging, with the double-headed arrow depicting the x-y layering of the acquired z-stack. (j) rendered (z-) stack of sections (n = 200, i.e. 400 μm depth) taken through the boxed region. (k) rendered stack of top 50 x-y sections (100 μm depth) taken from the region imaged around the notochord (comprising axial mesoderm and mesenchyme cells). (l) rendered stack of top 50 sections (100 μm depth) taken around the branchial pouch region (comprising endoderm and mesenchyme cells). The sections used to generate the rendered stacks in panels k and l were electronically magnified. Pink arrowheads, mitotic nuclei; yellow arrowheads, pycnotic nuclei; ect, ectoderm, en, endoderm, hf, headfold, mes, mesoderm, noto, notochord. |
PMC544401_F5_948.jpg | What can you see in this picture? | High resolution live imaging of the organs of CAG::H2B-EGFP adult mice. Confocal images of freshly isolated organs from a 6 week old adult male hemizygous CAG::H2B-EGFP Tg/+ animal illustrate the widespread nuclear localized expression of the histone fusion. A transverse cut was made through each organ and the cut surface was placed closest to the objective lens and imaged. Cell tracker orange was used as a vital cytoplasmic counter stain. The panels show rendered confocal z-stacks imaged through 80 μm of the brain using a 20x plan-apo objective (a-c), 568 μm of the heart using a 5x fluar objective (d-f), 142 μm of a lung lobe using a 5x fluar objective (g-i) and 346 μm of a kidney using a 5x fluar objective low power view (j-l), and high power view (m-o). Insets in panels a and d show the region of the brain and heart imaged, respectively. High resolution images of the kidney (m-o) illustrate electronic magnification of the data shown in j-l. Bron, bronchus; glom, glomeruus; med, medulla; sept, septum; ub, ureteric bud; ven, ventricle. Areas of increased fluorescence in the red channel are an artefact due to saturated pixels in regions of the sample closest to the objective. |
PMC544401_F5_943.jpg | What is shown in this image? | High resolution live imaging of the organs of CAG::H2B-EGFP adult mice. Confocal images of freshly isolated organs from a 6 week old adult male hemizygous CAG::H2B-EGFP Tg/+ animal illustrate the widespread nuclear localized expression of the histone fusion. A transverse cut was made through each organ and the cut surface was placed closest to the objective lens and imaged. Cell tracker orange was used as a vital cytoplasmic counter stain. The panels show rendered confocal z-stacks imaged through 80 μm of the brain using a 20x plan-apo objective (a-c), 568 μm of the heart using a 5x fluar objective (d-f), 142 μm of a lung lobe using a 5x fluar objective (g-i) and 346 μm of a kidney using a 5x fluar objective low power view (j-l), and high power view (m-o). Insets in panels a and d show the region of the brain and heart imaged, respectively. High resolution images of the kidney (m-o) illustrate electronic magnification of the data shown in j-l. Bron, bronchus; glom, glomeruus; med, medulla; sept, septum; ub, ureteric bud; ven, ventricle. Areas of increased fluorescence in the red channel are an artefact due to saturated pixels in regions of the sample closest to the objective. |
PMC544401_F5_946.jpg | What is the principal component of this image? | High resolution live imaging of the organs of CAG::H2B-EGFP adult mice. Confocal images of freshly isolated organs from a 6 week old adult male hemizygous CAG::H2B-EGFP Tg/+ animal illustrate the widespread nuclear localized expression of the histone fusion. A transverse cut was made through each organ and the cut surface was placed closest to the objective lens and imaged. Cell tracker orange was used as a vital cytoplasmic counter stain. The panels show rendered confocal z-stacks imaged through 80 μm of the brain using a 20x plan-apo objective (a-c), 568 μm of the heart using a 5x fluar objective (d-f), 142 μm of a lung lobe using a 5x fluar objective (g-i) and 346 μm of a kidney using a 5x fluar objective low power view (j-l), and high power view (m-o). Insets in panels a and d show the region of the brain and heart imaged, respectively. High resolution images of the kidney (m-o) illustrate electronic magnification of the data shown in j-l. Bron, bronchus; glom, glomeruus; med, medulla; sept, septum; ub, ureteric bud; ven, ventricle. Areas of increased fluorescence in the red channel are an artefact due to saturated pixels in regions of the sample closest to the objective. |
PMC544401_F5_945.jpg | What object or scene is depicted here? | High resolution live imaging of the organs of CAG::H2B-EGFP adult mice. Confocal images of freshly isolated organs from a 6 week old adult male hemizygous CAG::H2B-EGFP Tg/+ animal illustrate the widespread nuclear localized expression of the histone fusion. A transverse cut was made through each organ and the cut surface was placed closest to the objective lens and imaged. Cell tracker orange was used as a vital cytoplasmic counter stain. The panels show rendered confocal z-stacks imaged through 80 μm of the brain using a 20x plan-apo objective (a-c), 568 μm of the heart using a 5x fluar objective (d-f), 142 μm of a lung lobe using a 5x fluar objective (g-i) and 346 μm of a kidney using a 5x fluar objective low power view (j-l), and high power view (m-o). Insets in panels a and d show the region of the brain and heart imaged, respectively. High resolution images of the kidney (m-o) illustrate electronic magnification of the data shown in j-l. Bron, bronchus; glom, glomeruus; med, medulla; sept, septum; ub, ureteric bud; ven, ventricle. Areas of increased fluorescence in the red channel are an artefact due to saturated pixels in regions of the sample closest to the objective. |
PMC544401_F5_944.jpg | What object or scene is depicted here? | High resolution live imaging of the organs of CAG::H2B-EGFP adult mice. Confocal images of freshly isolated organs from a 6 week old adult male hemizygous CAG::H2B-EGFP Tg/+ animal illustrate the widespread nuclear localized expression of the histone fusion. A transverse cut was made through each organ and the cut surface was placed closest to the objective lens and imaged. Cell tracker orange was used as a vital cytoplasmic counter stain. The panels show rendered confocal z-stacks imaged through 80 μm of the brain using a 20x plan-apo objective (a-c), 568 μm of the heart using a 5x fluar objective (d-f), 142 μm of a lung lobe using a 5x fluar objective (g-i) and 346 μm of a kidney using a 5x fluar objective low power view (j-l), and high power view (m-o). Insets in panels a and d show the region of the brain and heart imaged, respectively. High resolution images of the kidney (m-o) illustrate electronic magnification of the data shown in j-l. Bron, bronchus; glom, glomeruus; med, medulla; sept, septum; ub, ureteric bud; ven, ventricle. Areas of increased fluorescence in the red channel are an artefact due to saturated pixels in regions of the sample closest to the objective. |
PMC544401_F5_951.jpg | Can you identify the primary element in this image? | High resolution live imaging of the organs of CAG::H2B-EGFP adult mice. Confocal images of freshly isolated organs from a 6 week old adult male hemizygous CAG::H2B-EGFP Tg/+ animal illustrate the widespread nuclear localized expression of the histone fusion. A transverse cut was made through each organ and the cut surface was placed closest to the objective lens and imaged. Cell tracker orange was used as a vital cytoplasmic counter stain. The panels show rendered confocal z-stacks imaged through 80 μm of the brain using a 20x plan-apo objective (a-c), 568 μm of the heart using a 5x fluar objective (d-f), 142 μm of a lung lobe using a 5x fluar objective (g-i) and 346 μm of a kidney using a 5x fluar objective low power view (j-l), and high power view (m-o). Insets in panels a and d show the region of the brain and heart imaged, respectively. High resolution images of the kidney (m-o) illustrate electronic magnification of the data shown in j-l. Bron, bronchus; glom, glomeruus; med, medulla; sept, septum; ub, ureteric bud; ven, ventricle. Areas of increased fluorescence in the red channel are an artefact due to saturated pixels in regions of the sample closest to the objective. |
PMC544401_F5_947.jpg | What is the dominant medical problem in this image? | High resolution live imaging of the organs of CAG::H2B-EGFP adult mice. Confocal images of freshly isolated organs from a 6 week old adult male hemizygous CAG::H2B-EGFP Tg/+ animal illustrate the widespread nuclear localized expression of the histone fusion. A transverse cut was made through each organ and the cut surface was placed closest to the objective lens and imaged. Cell tracker orange was used as a vital cytoplasmic counter stain. The panels show rendered confocal z-stacks imaged through 80 μm of the brain using a 20x plan-apo objective (a-c), 568 μm of the heart using a 5x fluar objective (d-f), 142 μm of a lung lobe using a 5x fluar objective (g-i) and 346 μm of a kidney using a 5x fluar objective low power view (j-l), and high power view (m-o). Insets in panels a and d show the region of the brain and heart imaged, respectively. High resolution images of the kidney (m-o) illustrate electronic magnification of the data shown in j-l. Bron, bronchus; glom, glomeruus; med, medulla; sept, septum; ub, ureteric bud; ven, ventricle. Areas of increased fluorescence in the red channel are an artefact due to saturated pixels in regions of the sample closest to the objective. |
PMC544401_F5_949.jpg | What is the dominant medical problem in this image? | High resolution live imaging of the organs of CAG::H2B-EGFP adult mice. Confocal images of freshly isolated organs from a 6 week old adult male hemizygous CAG::H2B-EGFP Tg/+ animal illustrate the widespread nuclear localized expression of the histone fusion. A transverse cut was made through each organ and the cut surface was placed closest to the objective lens and imaged. Cell tracker orange was used as a vital cytoplasmic counter stain. The panels show rendered confocal z-stacks imaged through 80 μm of the brain using a 20x plan-apo objective (a-c), 568 μm of the heart using a 5x fluar objective (d-f), 142 μm of a lung lobe using a 5x fluar objective (g-i) and 346 μm of a kidney using a 5x fluar objective low power view (j-l), and high power view (m-o). Insets in panels a and d show the region of the brain and heart imaged, respectively. High resolution images of the kidney (m-o) illustrate electronic magnification of the data shown in j-l. Bron, bronchus; glom, glomeruus; med, medulla; sept, septum; ub, ureteric bud; ven, ventricle. Areas of increased fluorescence in the red channel are an artefact due to saturated pixels in regions of the sample closest to the objective. |
PMC544401_F5_942.jpg | What's the most prominent thing you notice in this picture? | High resolution live imaging of the organs of CAG::H2B-EGFP adult mice. Confocal images of freshly isolated organs from a 6 week old adult male hemizygous CAG::H2B-EGFP Tg/+ animal illustrate the widespread nuclear localized expression of the histone fusion. A transverse cut was made through each organ and the cut surface was placed closest to the objective lens and imaged. Cell tracker orange was used as a vital cytoplasmic counter stain. The panels show rendered confocal z-stacks imaged through 80 μm of the brain using a 20x plan-apo objective (a-c), 568 μm of the heart using a 5x fluar objective (d-f), 142 μm of a lung lobe using a 5x fluar objective (g-i) and 346 μm of a kidney using a 5x fluar objective low power view (j-l), and high power view (m-o). Insets in panels a and d show the region of the brain and heart imaged, respectively. High resolution images of the kidney (m-o) illustrate electronic magnification of the data shown in j-l. Bron, bronchus; glom, glomeruus; med, medulla; sept, septum; ub, ureteric bud; ven, ventricle. Areas of increased fluorescence in the red channel are an artefact due to saturated pixels in regions of the sample closest to the objective. |
PMC544551_pbio-0030066-g007_964.jpg | What object or scene is depicted here? | Knockdown of patched Function Rescues Slow Muscle Defects in you
After injection of 420 pg of a mismatch control ptc1 MO, adaxial expression of myod (A) and Engrailed (B) was normal in wild-type embryos, but absent in you mutant embryos (C and D). When injected with 420 pg of a MO targeting ptc1, however, myod expression in mutants (E) was rescued to levels comparable to wild-type embryos (G). Engrailed expression was slightly expanded in both wild-type (F) and mutant (H) embryos injected with 420 pg of ptc1 MOs. Both adaxial myod expression and Engrailed expression was slightly expanded in wild-type (I and J) and you mutant embryos (K and L) injected with MOs targeting both ptc1 and ptc2 (420 pg each). Embryos assayed for myod expression are shown in flat mount at the 12-somite stage, and somites 5–9 of Engrailed-expressing embryos are shown in lateral view at 24 hpf. Anterior is to the left in all panels. Genotypes of all embryos were determined by PCR after photography. |
PMC544551_pbio-0030066-g007_970.jpg | What is the central feature of this picture? | Knockdown of patched Function Rescues Slow Muscle Defects in you
After injection of 420 pg of a mismatch control ptc1 MO, adaxial expression of myod (A) and Engrailed (B) was normal in wild-type embryos, but absent in you mutant embryos (C and D). When injected with 420 pg of a MO targeting ptc1, however, myod expression in mutants (E) was rescued to levels comparable to wild-type embryos (G). Engrailed expression was slightly expanded in both wild-type (F) and mutant (H) embryos injected with 420 pg of ptc1 MOs. Both adaxial myod expression and Engrailed expression was slightly expanded in wild-type (I and J) and you mutant embryos (K and L) injected with MOs targeting both ptc1 and ptc2 (420 pg each). Embryos assayed for myod expression are shown in flat mount at the 12-somite stage, and somites 5–9 of Engrailed-expressing embryos are shown in lateral view at 24 hpf. Anterior is to the left in all panels. Genotypes of all embryos were determined by PCR after photography. |
PMC544551_pbio-0030066-g007_965.jpg | Describe the main subject of this image. | Knockdown of patched Function Rescues Slow Muscle Defects in you
After injection of 420 pg of a mismatch control ptc1 MO, adaxial expression of myod (A) and Engrailed (B) was normal in wild-type embryos, but absent in you mutant embryos (C and D). When injected with 420 pg of a MO targeting ptc1, however, myod expression in mutants (E) was rescued to levels comparable to wild-type embryos (G). Engrailed expression was slightly expanded in both wild-type (F) and mutant (H) embryos injected with 420 pg of ptc1 MOs. Both adaxial myod expression and Engrailed expression was slightly expanded in wild-type (I and J) and you mutant embryos (K and L) injected with MOs targeting both ptc1 and ptc2 (420 pg each). Embryos assayed for myod expression are shown in flat mount at the 12-somite stage, and somites 5–9 of Engrailed-expressing embryos are shown in lateral view at 24 hpf. Anterior is to the left in all panels. Genotypes of all embryos were determined by PCR after photography. |
PMC544551_pbio-0030066-g007_967.jpg | What is the principal component of this image? | Knockdown of patched Function Rescues Slow Muscle Defects in you
After injection of 420 pg of a mismatch control ptc1 MO, adaxial expression of myod (A) and Engrailed (B) was normal in wild-type embryos, but absent in you mutant embryos (C and D). When injected with 420 pg of a MO targeting ptc1, however, myod expression in mutants (E) was rescued to levels comparable to wild-type embryos (G). Engrailed expression was slightly expanded in both wild-type (F) and mutant (H) embryos injected with 420 pg of ptc1 MOs. Both adaxial myod expression and Engrailed expression was slightly expanded in wild-type (I and J) and you mutant embryos (K and L) injected with MOs targeting both ptc1 and ptc2 (420 pg each). Embryos assayed for myod expression are shown in flat mount at the 12-somite stage, and somites 5–9 of Engrailed-expressing embryos are shown in lateral view at 24 hpf. Anterior is to the left in all panels. Genotypes of all embryos were determined by PCR after photography. |
PMC544551_pbio-0030066-g007_969.jpg | Can you identify the primary element in this image? | Knockdown of patched Function Rescues Slow Muscle Defects in you
After injection of 420 pg of a mismatch control ptc1 MO, adaxial expression of myod (A) and Engrailed (B) was normal in wild-type embryos, but absent in you mutant embryos (C and D). When injected with 420 pg of a MO targeting ptc1, however, myod expression in mutants (E) was rescued to levels comparable to wild-type embryos (G). Engrailed expression was slightly expanded in both wild-type (F) and mutant (H) embryos injected with 420 pg of ptc1 MOs. Both adaxial myod expression and Engrailed expression was slightly expanded in wild-type (I and J) and you mutant embryos (K and L) injected with MOs targeting both ptc1 and ptc2 (420 pg each). Embryos assayed for myod expression are shown in flat mount at the 12-somite stage, and somites 5–9 of Engrailed-expressing embryos are shown in lateral view at 24 hpf. Anterior is to the left in all panels. Genotypes of all embryos were determined by PCR after photography. |
PMC544560_F5_977.jpg | What is the core subject represented in this visual? | Delta protein expression is abnormally distributed in the presence of spen mutant clones. Crosses were performed as described in Figure 1, and third instar imaginal disks were isolated from progeny female larvae with the following genotypes: {MS1096 GAL4/+; 40 2piM FRT40A/ GFP FRT40A; UAS-Flp1/ +} (A-C), and {MS1096 GAL4/ +; spenpoc361 FRT40A/ GFP FRT40A; UAS-Flp1/ +} (D-I). Delta expression (in red) was detected in by using a mouse anti-Delta MAb followed by a Cy3-conjugated anti mouse antibody. The area indicated by the arrows in E and F is shown at higher magnification in G to I. Dorsal is up and anterior is to the left. |
PMC544560_F5_979.jpg | What is the central feature of this picture? | Delta protein expression is abnormally distributed in the presence of spen mutant clones. Crosses were performed as described in Figure 1, and third instar imaginal disks were isolated from progeny female larvae with the following genotypes: {MS1096 GAL4/+; 40 2piM FRT40A/ GFP FRT40A; UAS-Flp1/ +} (A-C), and {MS1096 GAL4/ +; spenpoc361 FRT40A/ GFP FRT40A; UAS-Flp1/ +} (D-I). Delta expression (in red) was detected in by using a mouse anti-Delta MAb followed by a Cy3-conjugated anti mouse antibody. The area indicated by the arrows in E and F is shown at higher magnification in G to I. Dorsal is up and anterior is to the left. |
PMC544560_F5_981.jpg | Can you identify the primary element in this image? | Delta protein expression is abnormally distributed in the presence of spen mutant clones. Crosses were performed as described in Figure 1, and third instar imaginal disks were isolated from progeny female larvae with the following genotypes: {MS1096 GAL4/+; 40 2piM FRT40A/ GFP FRT40A; UAS-Flp1/ +} (A-C), and {MS1096 GAL4/ +; spenpoc361 FRT40A/ GFP FRT40A; UAS-Flp1/ +} (D-I). Delta expression (in red) was detected in by using a mouse anti-Delta MAb followed by a Cy3-conjugated anti mouse antibody. The area indicated by the arrows in E and F is shown at higher magnification in G to I. Dorsal is up and anterior is to the left. |
PMC544560_F5_974.jpg | Describe the main subject of this image. | Delta protein expression is abnormally distributed in the presence of spen mutant clones. Crosses were performed as described in Figure 1, and third instar imaginal disks were isolated from progeny female larvae with the following genotypes: {MS1096 GAL4/+; 40 2piM FRT40A/ GFP FRT40A; UAS-Flp1/ +} (A-C), and {MS1096 GAL4/ +; spenpoc361 FRT40A/ GFP FRT40A; UAS-Flp1/ +} (D-I). Delta expression (in red) was detected in by using a mouse anti-Delta MAb followed by a Cy3-conjugated anti mouse antibody. The area indicated by the arrows in E and F is shown at higher magnification in G to I. Dorsal is up and anterior is to the left. |
PMC544560_F5_978.jpg | Describe the main subject of this image. | Delta protein expression is abnormally distributed in the presence of spen mutant clones. Crosses were performed as described in Figure 1, and third instar imaginal disks were isolated from progeny female larvae with the following genotypes: {MS1096 GAL4/+; 40 2piM FRT40A/ GFP FRT40A; UAS-Flp1/ +} (A-C), and {MS1096 GAL4/ +; spenpoc361 FRT40A/ GFP FRT40A; UAS-Flp1/ +} (D-I). Delta expression (in red) was detected in by using a mouse anti-Delta MAb followed by a Cy3-conjugated anti mouse antibody. The area indicated by the arrows in E and F is shown at higher magnification in G to I. Dorsal is up and anterior is to the left. |
PMC544560_F5_976.jpg | What object or scene is depicted here? | Delta protein expression is abnormally distributed in the presence of spen mutant clones. Crosses were performed as described in Figure 1, and third instar imaginal disks were isolated from progeny female larvae with the following genotypes: {MS1096 GAL4/+; 40 2piM FRT40A/ GFP FRT40A; UAS-Flp1/ +} (A-C), and {MS1096 GAL4/ +; spenpoc361 FRT40A/ GFP FRT40A; UAS-Flp1/ +} (D-I). Delta expression (in red) was detected in by using a mouse anti-Delta MAb followed by a Cy3-conjugated anti mouse antibody. The area indicated by the arrows in E and F is shown at higher magnification in G to I. Dorsal is up and anterior is to the left. |
PMC544560_F5_980.jpg | What object or scene is depicted here? | Delta protein expression is abnormally distributed in the presence of spen mutant clones. Crosses were performed as described in Figure 1, and third instar imaginal disks were isolated from progeny female larvae with the following genotypes: {MS1096 GAL4/+; 40 2piM FRT40A/ GFP FRT40A; UAS-Flp1/ +} (A-C), and {MS1096 GAL4/ +; spenpoc361 FRT40A/ GFP FRT40A; UAS-Flp1/ +} (D-I). Delta expression (in red) was detected in by using a mouse anti-Delta MAb followed by a Cy3-conjugated anti mouse antibody. The area indicated by the arrows in E and F is shown at higher magnification in G to I. Dorsal is up and anterior is to the left. |
PMC544560_F5_982.jpg | What's the most prominent thing you notice in this picture? | Delta protein expression is abnormally distributed in the presence of spen mutant clones. Crosses were performed as described in Figure 1, and third instar imaginal disks were isolated from progeny female larvae with the following genotypes: {MS1096 GAL4/+; 40 2piM FRT40A/ GFP FRT40A; UAS-Flp1/ +} (A-C), and {MS1096 GAL4/ +; spenpoc361 FRT40A/ GFP FRT40A; UAS-Flp1/ +} (D-I). Delta expression (in red) was detected in by using a mouse anti-Delta MAb followed by a Cy3-conjugated anti mouse antibody. The area indicated by the arrows in E and F is shown at higher magnification in G to I. Dorsal is up and anterior is to the left. |
PMC544560_F5_975.jpg | What can you see in this picture? | Delta protein expression is abnormally distributed in the presence of spen mutant clones. Crosses were performed as described in Figure 1, and third instar imaginal disks were isolated from progeny female larvae with the following genotypes: {MS1096 GAL4/+; 40 2piM FRT40A/ GFP FRT40A; UAS-Flp1/ +} (A-C), and {MS1096 GAL4/ +; spenpoc361 FRT40A/ GFP FRT40A; UAS-Flp1/ +} (D-I). Delta expression (in red) was detected in by using a mouse anti-Delta MAb followed by a Cy3-conjugated anti mouse antibody. The area indicated by the arrows in E and F is shown at higher magnification in G to I. Dorsal is up and anterior is to the left. |
PMC544560_F6_989.jpg | What is the principal component of this image? | Analysis of Cut protein expression in the presence of spen mutant clones. Crosses were performed as described in Figure 1, and third instar imaginal disks were isolated from progeny female larvae with the following genotypes: {MS1096 GAL4/+; 40 2piM FRT40A/ GFP FRT40A; UAS-Flp1/ +} (A-C), and {MS1096 GAL4/ +; spenpoc361 FRT40A/ GFP FRT40A; UAS-Flp1/ +} (D-L). Cut expression (in red) was detected with an anti-Cut MAb as described in Figure 4 for Dl, and the absence of GFP (in green) defines mutant cells as explained on Figure 4. G to H shows a magnification of the spot indicated by an arrow on E and F. Note that the Cut protein is present in spen mutant cells. Panels J to L show the margin of another disc not shown in the figure. The arrow indicates a group of heterozygous cells that are away from the margin, surrounded by a group on spen mutant cells. Dorsal is up and anterior is to the left. |
PMC544560_F6_988.jpg | What is the central feature of this picture? | Analysis of Cut protein expression in the presence of spen mutant clones. Crosses were performed as described in Figure 1, and third instar imaginal disks were isolated from progeny female larvae with the following genotypes: {MS1096 GAL4/+; 40 2piM FRT40A/ GFP FRT40A; UAS-Flp1/ +} (A-C), and {MS1096 GAL4/ +; spenpoc361 FRT40A/ GFP FRT40A; UAS-Flp1/ +} (D-L). Cut expression (in red) was detected with an anti-Cut MAb as described in Figure 4 for Dl, and the absence of GFP (in green) defines mutant cells as explained on Figure 4. G to H shows a magnification of the spot indicated by an arrow on E and F. Note that the Cut protein is present in spen mutant cells. Panels J to L show the margin of another disc not shown in the figure. The arrow indicates a group of heterozygous cells that are away from the margin, surrounded by a group on spen mutant cells. Dorsal is up and anterior is to the left. |
PMC544560_F6_990.jpg | What object or scene is depicted here? | Analysis of Cut protein expression in the presence of spen mutant clones. Crosses were performed as described in Figure 1, and third instar imaginal disks were isolated from progeny female larvae with the following genotypes: {MS1096 GAL4/+; 40 2piM FRT40A/ GFP FRT40A; UAS-Flp1/ +} (A-C), and {MS1096 GAL4/ +; spenpoc361 FRT40A/ GFP FRT40A; UAS-Flp1/ +} (D-L). Cut expression (in red) was detected with an anti-Cut MAb as described in Figure 4 for Dl, and the absence of GFP (in green) defines mutant cells as explained on Figure 4. G to H shows a magnification of the spot indicated by an arrow on E and F. Note that the Cut protein is present in spen mutant cells. Panels J to L show the margin of another disc not shown in the figure. The arrow indicates a group of heterozygous cells that are away from the margin, surrounded by a group on spen mutant cells. Dorsal is up and anterior is to the left. |
PMC544560_F6_991.jpg | What is the dominant medical problem in this image? | Analysis of Cut protein expression in the presence of spen mutant clones. Crosses were performed as described in Figure 1, and third instar imaginal disks were isolated from progeny female larvae with the following genotypes: {MS1096 GAL4/+; 40 2piM FRT40A/ GFP FRT40A; UAS-Flp1/ +} (A-C), and {MS1096 GAL4/ +; spenpoc361 FRT40A/ GFP FRT40A; UAS-Flp1/ +} (D-L). Cut expression (in red) was detected with an anti-Cut MAb as described in Figure 4 for Dl, and the absence of GFP (in green) defines mutant cells as explained on Figure 4. G to H shows a magnification of the spot indicated by an arrow on E and F. Note that the Cut protein is present in spen mutant cells. Panels J to L show the margin of another disc not shown in the figure. The arrow indicates a group of heterozygous cells that are away from the margin, surrounded by a group on spen mutant cells. Dorsal is up and anterior is to the left. |
PMC544560_F6_986.jpg | What's the most prominent thing you notice in this picture? | Analysis of Cut protein expression in the presence of spen mutant clones. Crosses were performed as described in Figure 1, and third instar imaginal disks were isolated from progeny female larvae with the following genotypes: {MS1096 GAL4/+; 40 2piM FRT40A/ GFP FRT40A; UAS-Flp1/ +} (A-C), and {MS1096 GAL4/ +; spenpoc361 FRT40A/ GFP FRT40A; UAS-Flp1/ +} (D-L). Cut expression (in red) was detected with an anti-Cut MAb as described in Figure 4 for Dl, and the absence of GFP (in green) defines mutant cells as explained on Figure 4. G to H shows a magnification of the spot indicated by an arrow on E and F. Note that the Cut protein is present in spen mutant cells. Panels J to L show the margin of another disc not shown in the figure. The arrow indicates a group of heterozygous cells that are away from the margin, surrounded by a group on spen mutant cells. Dorsal is up and anterior is to the left. |
PMC544560_F6_983.jpg | What is the main focus of this visual representation? | Analysis of Cut protein expression in the presence of spen mutant clones. Crosses were performed as described in Figure 1, and third instar imaginal disks were isolated from progeny female larvae with the following genotypes: {MS1096 GAL4/+; 40 2piM FRT40A/ GFP FRT40A; UAS-Flp1/ +} (A-C), and {MS1096 GAL4/ +; spenpoc361 FRT40A/ GFP FRT40A; UAS-Flp1/ +} (D-L). Cut expression (in red) was detected with an anti-Cut MAb as described in Figure 4 for Dl, and the absence of GFP (in green) defines mutant cells as explained on Figure 4. G to H shows a magnification of the spot indicated by an arrow on E and F. Note that the Cut protein is present in spen mutant cells. Panels J to L show the margin of another disc not shown in the figure. The arrow indicates a group of heterozygous cells that are away from the margin, surrounded by a group on spen mutant cells. Dorsal is up and anterior is to the left. |
PMC544560_F6_985.jpg | What does this image primarily show? | Analysis of Cut protein expression in the presence of spen mutant clones. Crosses were performed as described in Figure 1, and third instar imaginal disks were isolated from progeny female larvae with the following genotypes: {MS1096 GAL4/+; 40 2piM FRT40A/ GFP FRT40A; UAS-Flp1/ +} (A-C), and {MS1096 GAL4/ +; spenpoc361 FRT40A/ GFP FRT40A; UAS-Flp1/ +} (D-L). Cut expression (in red) was detected with an anti-Cut MAb as described in Figure 4 for Dl, and the absence of GFP (in green) defines mutant cells as explained on Figure 4. G to H shows a magnification of the spot indicated by an arrow on E and F. Note that the Cut protein is present in spen mutant cells. Panels J to L show the margin of another disc not shown in the figure. The arrow indicates a group of heterozygous cells that are away from the margin, surrounded by a group on spen mutant cells. Dorsal is up and anterior is to the left. |
PMC544570_F2_1006.jpg | What is the focal point of this photograph? | Inhibition of the interphase progression following surgical removal of the spindle midzone structures. An NRK cell was cut at anaphase to form daughter cells with (top) and without (bottom) the spindle midzone structures (cutting site indicated by the dotted line, a). Subsequent time-lapse recording indicates that both daughter cells formed nuclear envelop (arrows, d). However, only the daughter cell with spindle midzone (top) showed cytokinesis-like contractions (c, arrows). The daughter cell with spindle midzone entered the subsequent mitosis 11 hours after microsurgery (e, arrow), whereas the daughter cell without spindle midzone entered mitosis 22 h after microsurgery (f, arrow). Time elapsed since cutting is shown in hours:minutes. Bar, 20 μm. |
PMC544570_F2_1007.jpg | What does this image primarily show? | Inhibition of the interphase progression following surgical removal of the spindle midzone structures. An NRK cell was cut at anaphase to form daughter cells with (top) and without (bottom) the spindle midzone structures (cutting site indicated by the dotted line, a). Subsequent time-lapse recording indicates that both daughter cells formed nuclear envelop (arrows, d). However, only the daughter cell with spindle midzone (top) showed cytokinesis-like contractions (c, arrows). The daughter cell with spindle midzone entered the subsequent mitosis 11 hours after microsurgery (e, arrow), whereas the daughter cell without spindle midzone entered mitosis 22 h after microsurgery (f, arrow). Time elapsed since cutting is shown in hours:minutes. Bar, 20 μm. |
PMC544570_F2_1004.jpg | What's the most prominent thing you notice in this picture? | Inhibition of the interphase progression following surgical removal of the spindle midzone structures. An NRK cell was cut at anaphase to form daughter cells with (top) and without (bottom) the spindle midzone structures (cutting site indicated by the dotted line, a). Subsequent time-lapse recording indicates that both daughter cells formed nuclear envelop (arrows, d). However, only the daughter cell with spindle midzone (top) showed cytokinesis-like contractions (c, arrows). The daughter cell with spindle midzone entered the subsequent mitosis 11 hours after microsurgery (e, arrow), whereas the daughter cell without spindle midzone entered mitosis 22 h after microsurgery (f, arrow). Time elapsed since cutting is shown in hours:minutes. Bar, 20 μm. |
PMC544570_F2_1008.jpg | What is being portrayed in this visual content? | Inhibition of the interphase progression following surgical removal of the spindle midzone structures. An NRK cell was cut at anaphase to form daughter cells with (top) and without (bottom) the spindle midzone structures (cutting site indicated by the dotted line, a). Subsequent time-lapse recording indicates that both daughter cells formed nuclear envelop (arrows, d). However, only the daughter cell with spindle midzone (top) showed cytokinesis-like contractions (c, arrows). The daughter cell with spindle midzone entered the subsequent mitosis 11 hours after microsurgery (e, arrow), whereas the daughter cell without spindle midzone entered mitosis 22 h after microsurgery (f, arrow). Time elapsed since cutting is shown in hours:minutes. Bar, 20 μm. |
PMC544570_F3_993.jpg | What object or scene is depicted here? | Normal interphase progression following manipulation of the size of a daughter cell. A lateral region near the pole was cut away from one of the daughter cells at the end of cytokinesis (cutting site indicated by the dotted line, a). Subsequent time lapse imaging revealed a normal interphase duration in both daughter cells (b, c). Time elapsed since cutting is shown in hours:minutes. Bar, 20 μm. |
PMC544570_F3_995.jpg | What key item or scene is captured in this photo? | Normal interphase progression following manipulation of the size of a daughter cell. A lateral region near the pole was cut away from one of the daughter cells at the end of cytokinesis (cutting site indicated by the dotted line, a). Subsequent time lapse imaging revealed a normal interphase duration in both daughter cells (b, c). Time elapsed since cutting is shown in hours:minutes. Bar, 20 μm. |
PMC544570_F3_994.jpg | What is the principal component of this image? | Normal interphase progression following manipulation of the size of a daughter cell. A lateral region near the pole was cut away from one of the daughter cells at the end of cytokinesis (cutting site indicated by the dotted line, a). Subsequent time lapse imaging revealed a normal interphase duration in both daughter cells (b, c). Time elapsed since cutting is shown in hours:minutes. Bar, 20 μm. |
PMC544570_F5_998.jpg | What stands out most in this visual? | Normal interphase progression following removal of the midbody. A cell was cut at the end of cytokinesis to form daughter cells with or without the midbody (A, b, arrow). Subsequent long-term time-lapse imaging indicated a normal phase morphology for both cells (A). The cell with midbody entered mitosis 10 h 40 min after cutting, whereas the cell without midbody entered mitosis ~1 h afterwards (e, f, large arrows). Fluorescence imaging of aurora-B-GFP, a midbody component, confirmed that the midbody is completely segregated into one of the daughter cells (B; cutting indicated by dotted lines) Bar, 20 μm. |
PMC544570_F5_996.jpg | What is being portrayed in this visual content? | Normal interphase progression following removal of the midbody. A cell was cut at the end of cytokinesis to form daughter cells with or without the midbody (A, b, arrow). Subsequent long-term time-lapse imaging indicated a normal phase morphology for both cells (A). The cell with midbody entered mitosis 10 h 40 min after cutting, whereas the cell without midbody entered mitosis ~1 h afterwards (e, f, large arrows). Fluorescence imaging of aurora-B-GFP, a midbody component, confirmed that the midbody is completely segregated into one of the daughter cells (B; cutting indicated by dotted lines) Bar, 20 μm. |
PMC544570_F5_997.jpg | What is the main focus of this visual representation? | Normal interphase progression following removal of the midbody. A cell was cut at the end of cytokinesis to form daughter cells with or without the midbody (A, b, arrow). Subsequent long-term time-lapse imaging indicated a normal phase morphology for both cells (A). The cell with midbody entered mitosis 10 h 40 min after cutting, whereas the cell without midbody entered mitosis ~1 h afterwards (e, f, large arrows). Fluorescence imaging of aurora-B-GFP, a midbody component, confirmed that the midbody is completely segregated into one of the daughter cells (B; cutting indicated by dotted lines) Bar, 20 μm. |
PMC544570_F5_999.jpg | What is the focal point of this photograph? | Normal interphase progression following removal of the midbody. A cell was cut at the end of cytokinesis to form daughter cells with or without the midbody (A, b, arrow). Subsequent long-term time-lapse imaging indicated a normal phase morphology for both cells (A). The cell with midbody entered mitosis 10 h 40 min after cutting, whereas the cell without midbody entered mitosis ~1 h afterwards (e, f, large arrows). Fluorescence imaging of aurora-B-GFP, a midbody component, confirmed that the midbody is completely segregated into one of the daughter cells (B; cutting indicated by dotted lines) Bar, 20 μm. |
PMC544570_F5_1002.jpg | What is the main focus of this visual representation? | Normal interphase progression following removal of the midbody. A cell was cut at the end of cytokinesis to form daughter cells with or without the midbody (A, b, arrow). Subsequent long-term time-lapse imaging indicated a normal phase morphology for both cells (A). The cell with midbody entered mitosis 10 h 40 min after cutting, whereas the cell without midbody entered mitosis ~1 h afterwards (e, f, large arrows). Fluorescence imaging of aurora-B-GFP, a midbody component, confirmed that the midbody is completely segregated into one of the daughter cells (B; cutting indicated by dotted lines) Bar, 20 μm. |
PMC544570_F5_1000.jpg | What's the most prominent thing you notice in this picture? | Normal interphase progression following removal of the midbody. A cell was cut at the end of cytokinesis to form daughter cells with or without the midbody (A, b, arrow). Subsequent long-term time-lapse imaging indicated a normal phase morphology for both cells (A). The cell with midbody entered mitosis 10 h 40 min after cutting, whereas the cell without midbody entered mitosis ~1 h afterwards (e, f, large arrows). Fluorescence imaging of aurora-B-GFP, a midbody component, confirmed that the midbody is completely segregated into one of the daughter cells (B; cutting indicated by dotted lines) Bar, 20 μm. |
PMC544582_F1_1010.jpg | Can you identify the primary element in this image? | Effect of androstendione on endometrial cell proliferation and apoptosis of human endometria. Basal condition (left panel) and androstenedione-treated explants (right panel). The nucleolar antigen Ki-67, evaluated by immunohistochemistry, was detected in the nucleus of both cell compartments (A, D), indicative of cell proliferation. The nucleus of positive cells for TUNEL, determined by TdT-mediated dUTP nick end labeling, were stained in yellow and counterstained with propidium iodide (B, E), showing DNA fragmentation. The positive staining for caspase-3, determined by immunohistochemistry in paraffin wax sections of endometria, was found in the cell cytoplasm of both compartments (C, G). Negative controls (inserts) for inmunohistochemistry was performed with non-immune rabbit serum in the place of the respective primary antibody and for TUNEL, by the replacement of TdT enzyme. Arrowheads indicate positive staining of the respective proteins. Magnification in all panels, ×400. |
PMC544582_F1_1015.jpg | What can you see in this picture? | Effect of androstendione on endometrial cell proliferation and apoptosis of human endometria. Basal condition (left panel) and androstenedione-treated explants (right panel). The nucleolar antigen Ki-67, evaluated by immunohistochemistry, was detected in the nucleus of both cell compartments (A, D), indicative of cell proliferation. The nucleus of positive cells for TUNEL, determined by TdT-mediated dUTP nick end labeling, were stained in yellow and counterstained with propidium iodide (B, E), showing DNA fragmentation. The positive staining for caspase-3, determined by immunohistochemistry in paraffin wax sections of endometria, was found in the cell cytoplasm of both compartments (C, G). Negative controls (inserts) for inmunohistochemistry was performed with non-immune rabbit serum in the place of the respective primary antibody and for TUNEL, by the replacement of TdT enzyme. Arrowheads indicate positive staining of the respective proteins. Magnification in all panels, ×400. |
PMC544582_F1_1012.jpg | What is the dominant medical problem in this image? | Effect of androstendione on endometrial cell proliferation and apoptosis of human endometria. Basal condition (left panel) and androstenedione-treated explants (right panel). The nucleolar antigen Ki-67, evaluated by immunohistochemistry, was detected in the nucleus of both cell compartments (A, D), indicative of cell proliferation. The nucleus of positive cells for TUNEL, determined by TdT-mediated dUTP nick end labeling, were stained in yellow and counterstained with propidium iodide (B, E), showing DNA fragmentation. The positive staining for caspase-3, determined by immunohistochemistry in paraffin wax sections of endometria, was found in the cell cytoplasm of both compartments (C, G). Negative controls (inserts) for inmunohistochemistry was performed with non-immune rabbit serum in the place of the respective primary antibody and for TUNEL, by the replacement of TdT enzyme. Arrowheads indicate positive staining of the respective proteins. Magnification in all panels, ×400. |
PMC544582_F1_1013.jpg | What object or scene is depicted here? | Effect of androstendione on endometrial cell proliferation and apoptosis of human endometria. Basal condition (left panel) and androstenedione-treated explants (right panel). The nucleolar antigen Ki-67, evaluated by immunohistochemistry, was detected in the nucleus of both cell compartments (A, D), indicative of cell proliferation. The nucleus of positive cells for TUNEL, determined by TdT-mediated dUTP nick end labeling, were stained in yellow and counterstained with propidium iodide (B, E), showing DNA fragmentation. The positive staining for caspase-3, determined by immunohistochemistry in paraffin wax sections of endometria, was found in the cell cytoplasm of both compartments (C, G). Negative controls (inserts) for inmunohistochemistry was performed with non-immune rabbit serum in the place of the respective primary antibody and for TUNEL, by the replacement of TdT enzyme. Arrowheads indicate positive staining of the respective proteins. Magnification in all panels, ×400. |
PMC544582_F1_1014.jpg | What is the central feature of this picture? | Effect of androstendione on endometrial cell proliferation and apoptosis of human endometria. Basal condition (left panel) and androstenedione-treated explants (right panel). The nucleolar antigen Ki-67, evaluated by immunohistochemistry, was detected in the nucleus of both cell compartments (A, D), indicative of cell proliferation. The nucleus of positive cells for TUNEL, determined by TdT-mediated dUTP nick end labeling, were stained in yellow and counterstained with propidium iodide (B, E), showing DNA fragmentation. The positive staining for caspase-3, determined by immunohistochemistry in paraffin wax sections of endometria, was found in the cell cytoplasm of both compartments (C, G). Negative controls (inserts) for inmunohistochemistry was performed with non-immune rabbit serum in the place of the respective primary antibody and for TUNEL, by the replacement of TdT enzyme. Arrowheads indicate positive staining of the respective proteins. Magnification in all panels, ×400. |
PMC544837_F3_1017.jpg | What stands out most in this visual? | CT scan of the chest of the patient. Multiple metastatic lesions were seen. |
PMC544837_F3_1016.jpg | What's the most prominent thing you notice in this picture? | CT scan of the chest of the patient. Multiple metastatic lesions were seen. |
PMC544837_F4_1018.jpg | What is the dominant medical problem in this image? | Histological view of the specimen showed "adenoid BCC". (Hematoxylene eosin staining, × 50 magnification) |
PMC544841_F3_1020.jpg | What is the main focus of this visual representation? | Zymography gels for patients with greater than 50% resection and less then 50% resection. Figures 3A and 3B are greater than 50% and Figures 3C and 3D are less than 50%. The gels in Figures 3A and 3C contained plasminogen and the gels in Figures 3B and 3D are the corresponding plasminogen-free control gels. The samples run were, Lane 1- uPA (low molecular weight standard 33 kD, 0.6 ng); Lane 2- tPA standard (65 kD, 1 ng); Lane 3- uPA (high molecular weight standard 54 kD, 0.75 ng); Lane 4–10 μl SeeBlue Plus2 pre-stained standard markers; Lanes 5–9 were washed membrane preparation (30 μg protein / lane); Lane 5 – start; Lane 6 – Res 0; Lane 7 – Rem 0; Lane 8 – Res End; and Lane 9 – Rem End. |
PMC544841_F3_1022.jpg | What is the principal component of this image? | Zymography gels for patients with greater than 50% resection and less then 50% resection. Figures 3A and 3B are greater than 50% and Figures 3C and 3D are less than 50%. The gels in Figures 3A and 3C contained plasminogen and the gels in Figures 3B and 3D are the corresponding plasminogen-free control gels. The samples run were, Lane 1- uPA (low molecular weight standard 33 kD, 0.6 ng); Lane 2- tPA standard (65 kD, 1 ng); Lane 3- uPA (high molecular weight standard 54 kD, 0.75 ng); Lane 4–10 μl SeeBlue Plus2 pre-stained standard markers; Lanes 5–9 were washed membrane preparation (30 μg protein / lane); Lane 5 – start; Lane 6 – Res 0; Lane 7 – Rem 0; Lane 8 – Res End; and Lane 9 – Rem End. |
PMC544864_F1_1028.jpg | Describe the main subject of this image. | Radiologic, cytologic and morphologic appearance of the tumor. 1: This computed-tomographic scan of the patient's cerebral mass shows a large cystic mass with peripheral enhancement at the solid portion which attached to the overlying dura; 2: In addition to scattered individual cells, variably sized clusters of neuronal cells were identified, all composed of cells with eccentrically located, occasionally binucleated hyperchromatic nuclei and abundant unipolar cytoplasm [original magnifications ×400]; 3: Occasional neuronal cells were binucleated (3a) while others showed bland nuclear features (3b) [original magnifications ×400]; 4: Scattered astroglial cells with more convoluted nuclear contours and less cytoplasm were also present. [original magnifications ×400]; 5: Typical histologic appearance of desmoplastic infantile ganglioglioma, showing scattered ganglion cells in a desmoplastic and fibroblastic, vaguely storiform background (original magnification ×200, inset ×400) |
PMC544864_F1_1025.jpg | What is the focal point of this photograph? | Radiologic, cytologic and morphologic appearance of the tumor. 1: This computed-tomographic scan of the patient's cerebral mass shows a large cystic mass with peripheral enhancement at the solid portion which attached to the overlying dura; 2: In addition to scattered individual cells, variably sized clusters of neuronal cells were identified, all composed of cells with eccentrically located, occasionally binucleated hyperchromatic nuclei and abundant unipolar cytoplasm [original magnifications ×400]; 3: Occasional neuronal cells were binucleated (3a) while others showed bland nuclear features (3b) [original magnifications ×400]; 4: Scattered astroglial cells with more convoluted nuclear contours and less cytoplasm were also present. [original magnifications ×400]; 5: Typical histologic appearance of desmoplastic infantile ganglioglioma, showing scattered ganglion cells in a desmoplastic and fibroblastic, vaguely storiform background (original magnification ×200, inset ×400) |
PMC544864_F1_1027.jpg | What is the focal point of this photograph? | Radiologic, cytologic and morphologic appearance of the tumor. 1: This computed-tomographic scan of the patient's cerebral mass shows a large cystic mass with peripheral enhancement at the solid portion which attached to the overlying dura; 2: In addition to scattered individual cells, variably sized clusters of neuronal cells were identified, all composed of cells with eccentrically located, occasionally binucleated hyperchromatic nuclei and abundant unipolar cytoplasm [original magnifications ×400]; 3: Occasional neuronal cells were binucleated (3a) while others showed bland nuclear features (3b) [original magnifications ×400]; 4: Scattered astroglial cells with more convoluted nuclear contours and less cytoplasm were also present. [original magnifications ×400]; 5: Typical histologic appearance of desmoplastic infantile ganglioglioma, showing scattered ganglion cells in a desmoplastic and fibroblastic, vaguely storiform background (original magnification ×200, inset ×400) |
PMC544864_F1_1023.jpg | What is the core subject represented in this visual? | Radiologic, cytologic and morphologic appearance of the tumor. 1: This computed-tomographic scan of the patient's cerebral mass shows a large cystic mass with peripheral enhancement at the solid portion which attached to the overlying dura; 2: In addition to scattered individual cells, variably sized clusters of neuronal cells were identified, all composed of cells with eccentrically located, occasionally binucleated hyperchromatic nuclei and abundant unipolar cytoplasm [original magnifications ×400]; 3: Occasional neuronal cells were binucleated (3a) while others showed bland nuclear features (3b) [original magnifications ×400]; 4: Scattered astroglial cells with more convoluted nuclear contours and less cytoplasm were also present. [original magnifications ×400]; 5: Typical histologic appearance of desmoplastic infantile ganglioglioma, showing scattered ganglion cells in a desmoplastic and fibroblastic, vaguely storiform background (original magnification ×200, inset ×400) |
PMC544864_F1_1024.jpg | What object or scene is depicted here? | Radiologic, cytologic and morphologic appearance of the tumor. 1: This computed-tomographic scan of the patient's cerebral mass shows a large cystic mass with peripheral enhancement at the solid portion which attached to the overlying dura; 2: In addition to scattered individual cells, variably sized clusters of neuronal cells were identified, all composed of cells with eccentrically located, occasionally binucleated hyperchromatic nuclei and abundant unipolar cytoplasm [original magnifications ×400]; 3: Occasional neuronal cells were binucleated (3a) while others showed bland nuclear features (3b) [original magnifications ×400]; 4: Scattered astroglial cells with more convoluted nuclear contours and less cytoplasm were also present. [original magnifications ×400]; 5: Typical histologic appearance of desmoplastic infantile ganglioglioma, showing scattered ganglion cells in a desmoplastic and fibroblastic, vaguely storiform background (original magnification ×200, inset ×400) |
PMC544864_F1_1026.jpg | What is the main focus of this visual representation? | Radiologic, cytologic and morphologic appearance of the tumor. 1: This computed-tomographic scan of the patient's cerebral mass shows a large cystic mass with peripheral enhancement at the solid portion which attached to the overlying dura; 2: In addition to scattered individual cells, variably sized clusters of neuronal cells were identified, all composed of cells with eccentrically located, occasionally binucleated hyperchromatic nuclei and abundant unipolar cytoplasm [original magnifications ×400]; 3: Occasional neuronal cells were binucleated (3a) while others showed bland nuclear features (3b) [original magnifications ×400]; 4: Scattered astroglial cells with more convoluted nuclear contours and less cytoplasm were also present. [original magnifications ×400]; 5: Typical histologic appearance of desmoplastic infantile ganglioglioma, showing scattered ganglion cells in a desmoplastic and fibroblastic, vaguely storiform background (original magnification ×200, inset ×400) |
PMC544880_F1_1029.jpg | What stands out most in this visual? | Vegetation on tricuspid valve by echocardiography. Arrow denotes the vegetation. |
PMC544880_F1_1030.jpg | What's the most prominent thing you notice in this picture? | Vegetation on tricuspid valve by echocardiography. Arrow denotes the vegetation. |
PMC544927_pbio-0030027-g001_1032.jpg | What object or scene is depicted here? | Monaural Sound Localization in PET Experiments Performed in the Three Groups of Subjects(A) CBF increases. Activations of the right striate and extrastriate cortices are observed in EBSP but not in the two other groups for the contrast of MSL minus its control task. Upper image series, sagittal slices; lower image series, coronal slices. X and Y coordinates refer to standardized stereotaxic space.(B) Behavioral data. Behavioral results in MSL task (with SE bars). The dashed lines represent the ideal performance, whereas the solid lines indicate the best linear fit to the observed localization performance. Negative angles on the abscissa correspond to the obstructed ear, while positive angles correspond to the unobstructed ear. Note the better performance of the EBSP group compared to the EBNP and SIG. |
PMC544927_pbio-0030027-g001_1035.jpg | What is shown in this image? | Monaural Sound Localization in PET Experiments Performed in the Three Groups of Subjects(A) CBF increases. Activations of the right striate and extrastriate cortices are observed in EBSP but not in the two other groups for the contrast of MSL minus its control task. Upper image series, sagittal slices; lower image series, coronal slices. X and Y coordinates refer to standardized stereotaxic space.(B) Behavioral data. Behavioral results in MSL task (with SE bars). The dashed lines represent the ideal performance, whereas the solid lines indicate the best linear fit to the observed localization performance. Negative angles on the abscissa correspond to the obstructed ear, while positive angles correspond to the unobstructed ear. Note the better performance of the EBSP group compared to the EBNP and SIG. |
PMC544927_pbio-0030027-g001_1037.jpg | What does this image primarily show? | Monaural Sound Localization in PET Experiments Performed in the Three Groups of Subjects(A) CBF increases. Activations of the right striate and extrastriate cortices are observed in EBSP but not in the two other groups for the contrast of MSL minus its control task. Upper image series, sagittal slices; lower image series, coronal slices. X and Y coordinates refer to standardized stereotaxic space.(B) Behavioral data. Behavioral results in MSL task (with SE bars). The dashed lines represent the ideal performance, whereas the solid lines indicate the best linear fit to the observed localization performance. Negative angles on the abscissa correspond to the obstructed ear, while positive angles correspond to the unobstructed ear. Note the better performance of the EBSP group compared to the EBNP and SIG. |
PMC544927_pbio-0030027-g001_1036.jpg | What is the dominant medical problem in this image? | Monaural Sound Localization in PET Experiments Performed in the Three Groups of Subjects(A) CBF increases. Activations of the right striate and extrastriate cortices are observed in EBSP but not in the two other groups for the contrast of MSL minus its control task. Upper image series, sagittal slices; lower image series, coronal slices. X and Y coordinates refer to standardized stereotaxic space.(B) Behavioral data. Behavioral results in MSL task (with SE bars). The dashed lines represent the ideal performance, whereas the solid lines indicate the best linear fit to the observed localization performance. Negative angles on the abscissa correspond to the obstructed ear, while positive angles correspond to the unobstructed ear. Note the better performance of the EBSP group compared to the EBNP and SIG. |
PMC544927_pbio-0030027-g001_1033.jpg | What is the dominant medical problem in this image? | Monaural Sound Localization in PET Experiments Performed in the Three Groups of Subjects(A) CBF increases. Activations of the right striate and extrastriate cortices are observed in EBSP but not in the two other groups for the contrast of MSL minus its control task. Upper image series, sagittal slices; lower image series, coronal slices. X and Y coordinates refer to standardized stereotaxic space.(B) Behavioral data. Behavioral results in MSL task (with SE bars). The dashed lines represent the ideal performance, whereas the solid lines indicate the best linear fit to the observed localization performance. Negative angles on the abscissa correspond to the obstructed ear, while positive angles correspond to the unobstructed ear. Note the better performance of the EBSP group compared to the EBNP and SIG. |
PMC544927_pbio-0030027-g001_1031.jpg | What is the focal point of this photograph? | Monaural Sound Localization in PET Experiments Performed in the Three Groups of Subjects(A) CBF increases. Activations of the right striate and extrastriate cortices are observed in EBSP but not in the two other groups for the contrast of MSL minus its control task. Upper image series, sagittal slices; lower image series, coronal slices. X and Y coordinates refer to standardized stereotaxic space.(B) Behavioral data. Behavioral results in MSL task (with SE bars). The dashed lines represent the ideal performance, whereas the solid lines indicate the best linear fit to the observed localization performance. Negative angles on the abscissa correspond to the obstructed ear, while positive angles correspond to the unobstructed ear. Note the better performance of the EBSP group compared to the EBNP and SIG. |
PMC544935_F2_1040.jpg | Describe the main subject of this image. | (A-C): Secondary invagination sites are surrounded by sheath cells. Electron micrographs of transverse sections through prosomal hemi-neuromeres. (A,B) Invagination sites (arrows) are surrounded by sheath cells (asterisks) that appear translucent in the electron microscope. The sheath cells extend processes (arrowhead) that enwrap the individual invagination sites. (C) Sheath cells that are located in the apical cell layer form bizarre shapes that extend into the cell free space at the ventral side of the embryo (arrowhead). The sheath cells are labeled with asterisks, the arrow points to an invagination site. |
PMC544935_F2_1038.jpg | What is the focal point of this photograph? | (A-C): Secondary invagination sites are surrounded by sheath cells. Electron micrographs of transverse sections through prosomal hemi-neuromeres. (A,B) Invagination sites (arrows) are surrounded by sheath cells (asterisks) that appear translucent in the electron microscope. The sheath cells extend processes (arrowhead) that enwrap the individual invagination sites. (C) Sheath cells that are located in the apical cell layer form bizarre shapes that extend into the cell free space at the ventral side of the embryo (arrowhead). The sheath cells are labeled with asterisks, the arrow points to an invagination site. |
PMC544935_F2_1039.jpg | What is the focal point of this photograph? | (A-C): Secondary invagination sites are surrounded by sheath cells. Electron micrographs of transverse sections through prosomal hemi-neuromeres. (A,B) Invagination sites (arrows) are surrounded by sheath cells (asterisks) that appear translucent in the electron microscope. The sheath cells extend processes (arrowhead) that enwrap the individual invagination sites. (C) Sheath cells that are located in the apical cell layer form bizarre shapes that extend into the cell free space at the ventral side of the embryo (arrowhead). The sheath cells are labeled with asterisks, the arrow points to an invagination site. |
PMC545044_F1_1042.jpg | What is the principal component of this image? | The results of chest HRCT examination in a SARS patient in the convalescent phase, showing marked reversal of pulmonary fibrosis. |
PMC545044_F1_1044.jpg | Can you identify the primary element in this image? | The results of chest HRCT examination in a SARS patient in the convalescent phase, showing marked reversal of pulmonary fibrosis. |
PMC545044_F1_1043.jpg | What is the dominant medical problem in this image? | The results of chest HRCT examination in a SARS patient in the convalescent phase, showing marked reversal of pulmonary fibrosis. |
PMC545044_F1_1046.jpg | What is the dominant medical problem in this image? | The results of chest HRCT examination in a SARS patient in the convalescent phase, showing marked reversal of pulmonary fibrosis. |
PMC545047_F1_1049.jpg | What's the most prominent thing you notice in this picture? | Immunohistochemical staining of VEGF-C in biopsied specimens (upper panel) and hematoxylin-eosin (H&E) staining, immunohistochemical staining of cytokeratin and VEGF-C in metastastic lymph node (middle and lower panel). a, VEGF-C positive type in biopsied specimens. b, VEGF-C negative type in biopsied specimens. c, f, hematoxylin-eosin (H&E) staining in metastastic lymph node. d, g, immunohistochemical staining of cytokeratin in metastastic lymph node. e, VEGF-C positive type in metastastic lymph node. h, VEGF-C negative type in metastastic lymph node. |
PMC545047_F1_1047.jpg | What key item or scene is captured in this photo? | Immunohistochemical staining of VEGF-C in biopsied specimens (upper panel) and hematoxylin-eosin (H&E) staining, immunohistochemical staining of cytokeratin and VEGF-C in metastastic lymph node (middle and lower panel). a, VEGF-C positive type in biopsied specimens. b, VEGF-C negative type in biopsied specimens. c, f, hematoxylin-eosin (H&E) staining in metastastic lymph node. d, g, immunohistochemical staining of cytokeratin in metastastic lymph node. e, VEGF-C positive type in metastastic lymph node. h, VEGF-C negative type in metastastic lymph node. |
PMC545047_F1_1048.jpg | What is shown in this image? | Immunohistochemical staining of VEGF-C in biopsied specimens (upper panel) and hematoxylin-eosin (H&E) staining, immunohistochemical staining of cytokeratin and VEGF-C in metastastic lymph node (middle and lower panel). a, VEGF-C positive type in biopsied specimens. b, VEGF-C negative type in biopsied specimens. c, f, hematoxylin-eosin (H&E) staining in metastastic lymph node. d, g, immunohistochemical staining of cytokeratin in metastastic lymph node. e, VEGF-C positive type in metastastic lymph node. h, VEGF-C negative type in metastastic lymph node. |
PMC545052_F1_1052.jpg | What stands out most in this visual? | CT scan image of the lung of case 3 patient. A: Before vaccination (May 15, 2003). B: After the third vaccination (June 18, 2003). Rapid growth of the metastatic tumors and pleural effusion were seen. |
PMC545052_F1_1051.jpg | What is the dominant medical problem in this image? | CT scan image of the lung of case 3 patient. A: Before vaccination (May 15, 2003). B: After the third vaccination (June 18, 2003). Rapid growth of the metastatic tumors and pleural effusion were seen. |
PMC545052_F2_1057.jpg | What is the dominant medical problem in this image? | CT scan image of the lung of case 5 patient. A: Before vaccination (July 8, 2003). B: After the first vaccination (July 22, 2003). C: After the third vaccination (August 19, 2003). D: After the sixth vaccination (September 16, 2003). The metastatic tumors appeared to be dormant after the first vaccination. |
PMC545052_F2_1054.jpg | What object or scene is depicted here? | CT scan image of the lung of case 5 patient. A: Before vaccination (July 8, 2003). B: After the first vaccination (July 22, 2003). C: After the third vaccination (August 19, 2003). D: After the sixth vaccination (September 16, 2003). The metastatic tumors appeared to be dormant after the first vaccination. |
PMC545052_F2_1055.jpg | What's the most prominent thing you notice in this picture? | CT scan image of the lung of case 5 patient. A: Before vaccination (July 8, 2003). B: After the first vaccination (July 22, 2003). C: After the third vaccination (August 19, 2003). D: After the sixth vaccination (September 16, 2003). The metastatic tumors appeared to be dormant after the first vaccination. |
PMC545056_F2_1058.jpg | What is the focal point of this photograph? | The histological examination of the removed specimen (right hemicolectomy specimen) revealed actinomyces colonies in the upper part of the cecum and the ascending colon wall. The large spherical clusters, densely packed and branching, and PAS positive "sulfur granules" are specific of actinomycosis. |
PMC545073_F2_1059.jpg | What is the main focus of this visual representation? | Isolated overexpression of Xmab21l2 produces partially dorsalized embryos. A, C, E: control-injected embryos; B, D, F: Xmab21l2-injected embryos. A–D: tailbud-tadpole stages; E, F: gastrula stage. In B: external appearance of Xmab21l2-injected embryos. D: horizontal section of an Xmab21l2-injected section stained with orange-G/aniline blue. Note enlarged notocord (nc). F: expanded and enhanced Chordin expression in Xmab21l2-injected gastrulae (blue signal). |
PMC545073_F2_1063.jpg | What's the most prominent thing you notice in this picture? | Isolated overexpression of Xmab21l2 produces partially dorsalized embryos. A, C, E: control-injected embryos; B, D, F: Xmab21l2-injected embryos. A–D: tailbud-tadpole stages; E, F: gastrula stage. In B: external appearance of Xmab21l2-injected embryos. D: horizontal section of an Xmab21l2-injected section stained with orange-G/aniline blue. Note enlarged notocord (nc). F: expanded and enhanced Chordin expression in Xmab21l2-injected gastrulae (blue signal). |
PMC545075_F2_1091.jpg | Can you identify the primary element in this image? | Identification of septal, outflow tract, and aortic arch malformations using multi-embryo MRI (a – e') Images of transverse sections from 5 Cited2-/- embryos obtained using the multi-embryo technique (a–e) compared with images from the same embryos obtained subsequently using the single embryo technique (a'–e'). (a, a') Section showing left and right atria and ventricles (la, ram, live, rave). The atria are separated by the primary atria septum (pas), which is deficient at its ventral margin creating an osmium premium type of atria septal defect (ASD-P). (b, b') Section showing a ventricular septal defect (VSD) in the interventricular septum (ivs). (c, c') Section showing double outlet right ventricle, wherein the ascending aorta (a-ao) and the pulmonary artery (pa) both arise from the right ventricle (rv). The aortic valve (ao-v) is indicated. (d, d') Section showing a right-sided aortic arch (ao-a) passing to the right of the trachea (tr) and the esophagus (es). (e, e') Section showing bilateral aortic arches (ao-a) forming a vascular ring around the trachea (tr) and the esophagus (es). Also indicated are the thymus (th) and the right superior vena cava (r-svc). (f – j) Serial transverse sections through a wild-type heart obtained using single embryo MRI, demonstrating corresponding normal structures, including the systemic venous sinus (svs), left superior vena cava (l-svc), pulmonary vein (pvn), descending aorta (d-ao), mitral and tricuspid valves (mv, tv), the secondary atrial septum (sas), left and right ventricular outflow tracts (lvot, rvot), pulmonary valve (pv), and arterial duct (ad) of the pulmonary artery. Scale bars = 635 μm for multi-embryo, and 317 μm for single embryo images; axes: d – dorsal; v – ventral; r – right; l – left. |
PMC545075_F2_1086.jpg | What stands out most in this visual? | Identification of septal, outflow tract, and aortic arch malformations using multi-embryo MRI (a – e') Images of transverse sections from 5 Cited2-/- embryos obtained using the multi-embryo technique (a–e) compared with images from the same embryos obtained subsequently using the single embryo technique (a'–e'). (a, a') Section showing left and right atria and ventricles (la, ram, live, rave). The atria are separated by the primary atria septum (pas), which is deficient at its ventral margin creating an osmium premium type of atria septal defect (ASD-P). (b, b') Section showing a ventricular septal defect (VSD) in the interventricular septum (ivs). (c, c') Section showing double outlet right ventricle, wherein the ascending aorta (a-ao) and the pulmonary artery (pa) both arise from the right ventricle (rv). The aortic valve (ao-v) is indicated. (d, d') Section showing a right-sided aortic arch (ao-a) passing to the right of the trachea (tr) and the esophagus (es). (e, e') Section showing bilateral aortic arches (ao-a) forming a vascular ring around the trachea (tr) and the esophagus (es). Also indicated are the thymus (th) and the right superior vena cava (r-svc). (f – j) Serial transverse sections through a wild-type heart obtained using single embryo MRI, demonstrating corresponding normal structures, including the systemic venous sinus (svs), left superior vena cava (l-svc), pulmonary vein (pvn), descending aorta (d-ao), mitral and tricuspid valves (mv, tv), the secondary atrial septum (sas), left and right ventricular outflow tracts (lvot, rvot), pulmonary valve (pv), and arterial duct (ad) of the pulmonary artery. Scale bars = 635 μm for multi-embryo, and 317 μm for single embryo images; axes: d – dorsal; v – ventral; r – right; l – left. |
Subsets and Splits