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PMC534809_pbio-0020424-g006_800.jpg | What is being portrayed in this visual content? | AFM Images of DAO-E Crystals(A) A large templated crystal in a 5-tile reaction (no R-11). A single ‘1' in the input row (asterisk) initiates a Sierpinski triangle, which subsequently devolves due to errors. Mismatch errors within ‘0' domains initiate isolated Sierpinski patterns terminated by additional errors at their corners.(B) A large untemplated fragment in a 5-tile reaction (no S-11). Large triangles of ‘0's can be seen. Crystals similar to this are also seen in samples lacking the nucleating structure.(C) Several large crystals in a 6-tile reaction, some with more zeros than ones, some with more ones than zeros. It is difficult to determine whether these crystals are templated or not.(D) An average of several scans of the boxed region from (C), containing roughly 1,000 tiles and 45 errors.(E) An average of several scans of a Sierpinski triangle that initiated by a single error in a sea of zeros and terminated by three further errors (a 1% error rate for the 400 tiles here). Red crosses in (D) and (E) indicate tiles that have been identified (by eye) to be incorrect with respect to the two tiles from which they receive their input. Scale bars are 100 nm. |
PMC534809_pbio-0020424-g006_798.jpg | What is the dominant medical problem in this image? | AFM Images of DAO-E Crystals(A) A large templated crystal in a 5-tile reaction (no R-11). A single ‘1' in the input row (asterisk) initiates a Sierpinski triangle, which subsequently devolves due to errors. Mismatch errors within ‘0' domains initiate isolated Sierpinski patterns terminated by additional errors at their corners.(B) A large untemplated fragment in a 5-tile reaction (no S-11). Large triangles of ‘0's can be seen. Crystals similar to this are also seen in samples lacking the nucleating structure.(C) Several large crystals in a 6-tile reaction, some with more zeros than ones, some with more ones than zeros. It is difficult to determine whether these crystals are templated or not.(D) An average of several scans of the boxed region from (C), containing roughly 1,000 tiles and 45 errors.(E) An average of several scans of a Sierpinski triangle that initiated by a single error in a sea of zeros and terminated by three further errors (a 1% error rate for the 400 tiles here). Red crosses in (D) and (E) indicate tiles that have been identified (by eye) to be incorrect with respect to the two tiles from which they receive their input. Scale bars are 100 nm. |
PMC534809_pbio-0020424-g006_799.jpg | What does this image primarily show? | AFM Images of DAO-E Crystals(A) A large templated crystal in a 5-tile reaction (no R-11). A single ‘1' in the input row (asterisk) initiates a Sierpinski triangle, which subsequently devolves due to errors. Mismatch errors within ‘0' domains initiate isolated Sierpinski patterns terminated by additional errors at their corners.(B) A large untemplated fragment in a 5-tile reaction (no S-11). Large triangles of ‘0's can be seen. Crystals similar to this are also seen in samples lacking the nucleating structure.(C) Several large crystals in a 6-tile reaction, some with more zeros than ones, some with more ones than zeros. It is difficult to determine whether these crystals are templated or not.(D) An average of several scans of the boxed region from (C), containing roughly 1,000 tiles and 45 errors.(E) An average of several scans of a Sierpinski triangle that initiated by a single error in a sea of zeros and terminated by three further errors (a 1% error rate for the 400 tiles here). Red crosses in (D) and (E) indicate tiles that have been identified (by eye) to be incorrect with respect to the two tiles from which they receive their input. Scale bars are 100 nm. |
PMC535354_F1_803.jpg | What is the main focus of this visual representation? | Coronal T2-weighted MRI reveals a slightly hyperintense fusiforme tumorous lesion of the median nerve approximately 5 cm above the right elbow (arrows). |
PMC535545_F4_818.jpg | What key item or scene is captured in this photo? | Immunohistochemistry for extracellular matrix components. Immunohistochemical staining of wild type (A, C, E, G, I, K, M, O, P, Q, S) and IL11Ra-/- (B, D, F, H, J, L, N, R, T) uterus at 48 h of decidualization using specific antibodies for collagen III (A, B, C, D), biglycan (E, F, G, H), nidogen-1 (I, J, K, L), SPARC (M, N, O, P) and desmin (Q, R, S, T). Negative controls using a matching concentration of non-immune IgG (collagen III, nidogen-1, SPARC and desmin) or normal serum (biglycan) in place of the primary antibody are inset in A, B, E, F, I, J, M, N, Q and R. Black squares on A and B indicate the antimesometrial pole magnified in C and D. Abbreviations: connective tissue (ct), myometrium (my), mesometrial pole (m), antimesometrial pole (am), luminal epithelium (le), glandular epithelium (ge), decidualized stromal cell (dsc), non-decidualized stromal cell (sc), blood vessel (bv), glycocalyx (gly). Scale bar = 50 μm (A, B, E, F, I, J, M, N, Q and R are at the same magnification; C, D, G and P are at the same magnification; H, K, L, O, S, T and inset in G are at the same magnification). |
PMC535545_F4_813.jpg | Can you identify the primary element in this image? | Immunohistochemistry for extracellular matrix components. Immunohistochemical staining of wild type (A, C, E, G, I, K, M, O, P, Q, S) and IL11Ra-/- (B, D, F, H, J, L, N, R, T) uterus at 48 h of decidualization using specific antibodies for collagen III (A, B, C, D), biglycan (E, F, G, H), nidogen-1 (I, J, K, L), SPARC (M, N, O, P) and desmin (Q, R, S, T). Negative controls using a matching concentration of non-immune IgG (collagen III, nidogen-1, SPARC and desmin) or normal serum (biglycan) in place of the primary antibody are inset in A, B, E, F, I, J, M, N, Q and R. Black squares on A and B indicate the antimesometrial pole magnified in C and D. Abbreviations: connective tissue (ct), myometrium (my), mesometrial pole (m), antimesometrial pole (am), luminal epithelium (le), glandular epithelium (ge), decidualized stromal cell (dsc), non-decidualized stromal cell (sc), blood vessel (bv), glycocalyx (gly). Scale bar = 50 μm (A, B, E, F, I, J, M, N, Q and R are at the same magnification; C, D, G and P are at the same magnification; H, K, L, O, S, T and inset in G are at the same magnification). |
PMC535545_F4_822.jpg | What is the principal component of this image? | Immunohistochemistry for extracellular matrix components. Immunohistochemical staining of wild type (A, C, E, G, I, K, M, O, P, Q, S) and IL11Ra-/- (B, D, F, H, J, L, N, R, T) uterus at 48 h of decidualization using specific antibodies for collagen III (A, B, C, D), biglycan (E, F, G, H), nidogen-1 (I, J, K, L), SPARC (M, N, O, P) and desmin (Q, R, S, T). Negative controls using a matching concentration of non-immune IgG (collagen III, nidogen-1, SPARC and desmin) or normal serum (biglycan) in place of the primary antibody are inset in A, B, E, F, I, J, M, N, Q and R. Black squares on A and B indicate the antimesometrial pole magnified in C and D. Abbreviations: connective tissue (ct), myometrium (my), mesometrial pole (m), antimesometrial pole (am), luminal epithelium (le), glandular epithelium (ge), decidualized stromal cell (dsc), non-decidualized stromal cell (sc), blood vessel (bv), glycocalyx (gly). Scale bar = 50 μm (A, B, E, F, I, J, M, N, Q and R are at the same magnification; C, D, G and P are at the same magnification; H, K, L, O, S, T and inset in G are at the same magnification). |
PMC535545_F4_812.jpg | What can you see in this picture? | Immunohistochemistry for extracellular matrix components. Immunohistochemical staining of wild type (A, C, E, G, I, K, M, O, P, Q, S) and IL11Ra-/- (B, D, F, H, J, L, N, R, T) uterus at 48 h of decidualization using specific antibodies for collagen III (A, B, C, D), biglycan (E, F, G, H), nidogen-1 (I, J, K, L), SPARC (M, N, O, P) and desmin (Q, R, S, T). Negative controls using a matching concentration of non-immune IgG (collagen III, nidogen-1, SPARC and desmin) or normal serum (biglycan) in place of the primary antibody are inset in A, B, E, F, I, J, M, N, Q and R. Black squares on A and B indicate the antimesometrial pole magnified in C and D. Abbreviations: connective tissue (ct), myometrium (my), mesometrial pole (m), antimesometrial pole (am), luminal epithelium (le), glandular epithelium (ge), decidualized stromal cell (dsc), non-decidualized stromal cell (sc), blood vessel (bv), glycocalyx (gly). Scale bar = 50 μm (A, B, E, F, I, J, M, N, Q and R are at the same magnification; C, D, G and P are at the same magnification; H, K, L, O, S, T and inset in G are at the same magnification). |
PMC535545_F4_807.jpg | What is shown in this image? | Immunohistochemistry for extracellular matrix components. Immunohistochemical staining of wild type (A, C, E, G, I, K, M, O, P, Q, S) and IL11Ra-/- (B, D, F, H, J, L, N, R, T) uterus at 48 h of decidualization using specific antibodies for collagen III (A, B, C, D), biglycan (E, F, G, H), nidogen-1 (I, J, K, L), SPARC (M, N, O, P) and desmin (Q, R, S, T). Negative controls using a matching concentration of non-immune IgG (collagen III, nidogen-1, SPARC and desmin) or normal serum (biglycan) in place of the primary antibody are inset in A, B, E, F, I, J, M, N, Q and R. Black squares on A and B indicate the antimesometrial pole magnified in C and D. Abbreviations: connective tissue (ct), myometrium (my), mesometrial pole (m), antimesometrial pole (am), luminal epithelium (le), glandular epithelium (ge), decidualized stromal cell (dsc), non-decidualized stromal cell (sc), blood vessel (bv), glycocalyx (gly). Scale bar = 50 μm (A, B, E, F, I, J, M, N, Q and R are at the same magnification; C, D, G and P are at the same magnification; H, K, L, O, S, T and inset in G are at the same magnification). |
PMC535545_F4_814.jpg | What key item or scene is captured in this photo? | Immunohistochemistry for extracellular matrix components. Immunohistochemical staining of wild type (A, C, E, G, I, K, M, O, P, Q, S) and IL11Ra-/- (B, D, F, H, J, L, N, R, T) uterus at 48 h of decidualization using specific antibodies for collagen III (A, B, C, D), biglycan (E, F, G, H), nidogen-1 (I, J, K, L), SPARC (M, N, O, P) and desmin (Q, R, S, T). Negative controls using a matching concentration of non-immune IgG (collagen III, nidogen-1, SPARC and desmin) or normal serum (biglycan) in place of the primary antibody are inset in A, B, E, F, I, J, M, N, Q and R. Black squares on A and B indicate the antimesometrial pole magnified in C and D. Abbreviations: connective tissue (ct), myometrium (my), mesometrial pole (m), antimesometrial pole (am), luminal epithelium (le), glandular epithelium (ge), decidualized stromal cell (dsc), non-decidualized stromal cell (sc), blood vessel (bv), glycocalyx (gly). Scale bar = 50 μm (A, B, E, F, I, J, M, N, Q and R are at the same magnification; C, D, G and P are at the same magnification; H, K, L, O, S, T and inset in G are at the same magnification). |
PMC535545_F4_816.jpg | What is the dominant medical problem in this image? | Immunohistochemistry for extracellular matrix components. Immunohistochemical staining of wild type (A, C, E, G, I, K, M, O, P, Q, S) and IL11Ra-/- (B, D, F, H, J, L, N, R, T) uterus at 48 h of decidualization using specific antibodies for collagen III (A, B, C, D), biglycan (E, F, G, H), nidogen-1 (I, J, K, L), SPARC (M, N, O, P) and desmin (Q, R, S, T). Negative controls using a matching concentration of non-immune IgG (collagen III, nidogen-1, SPARC and desmin) or normal serum (biglycan) in place of the primary antibody are inset in A, B, E, F, I, J, M, N, Q and R. Black squares on A and B indicate the antimesometrial pole magnified in C and D. Abbreviations: connective tissue (ct), myometrium (my), mesometrial pole (m), antimesometrial pole (am), luminal epithelium (le), glandular epithelium (ge), decidualized stromal cell (dsc), non-decidualized stromal cell (sc), blood vessel (bv), glycocalyx (gly). Scale bar = 50 μm (A, B, E, F, I, J, M, N, Q and R are at the same magnification; C, D, G and P are at the same magnification; H, K, L, O, S, T and inset in G are at the same magnification). |
PMC535545_F4_809.jpg | What is the core subject represented in this visual? | Immunohistochemistry for extracellular matrix components. Immunohistochemical staining of wild type (A, C, E, G, I, K, M, O, P, Q, S) and IL11Ra-/- (B, D, F, H, J, L, N, R, T) uterus at 48 h of decidualization using specific antibodies for collagen III (A, B, C, D), biglycan (E, F, G, H), nidogen-1 (I, J, K, L), SPARC (M, N, O, P) and desmin (Q, R, S, T). Negative controls using a matching concentration of non-immune IgG (collagen III, nidogen-1, SPARC and desmin) or normal serum (biglycan) in place of the primary antibody are inset in A, B, E, F, I, J, M, N, Q and R. Black squares on A and B indicate the antimesometrial pole magnified in C and D. Abbreviations: connective tissue (ct), myometrium (my), mesometrial pole (m), antimesometrial pole (am), luminal epithelium (le), glandular epithelium (ge), decidualized stromal cell (dsc), non-decidualized stromal cell (sc), blood vessel (bv), glycocalyx (gly). Scale bar = 50 μm (A, B, E, F, I, J, M, N, Q and R are at the same magnification; C, D, G and P are at the same magnification; H, K, L, O, S, T and inset in G are at the same magnification). |
PMC535545_F4_819.jpg | Can you identify the primary element in this image? | Immunohistochemistry for extracellular matrix components. Immunohistochemical staining of wild type (A, C, E, G, I, K, M, O, P, Q, S) and IL11Ra-/- (B, D, F, H, J, L, N, R, T) uterus at 48 h of decidualization using specific antibodies for collagen III (A, B, C, D), biglycan (E, F, G, H), nidogen-1 (I, J, K, L), SPARC (M, N, O, P) and desmin (Q, R, S, T). Negative controls using a matching concentration of non-immune IgG (collagen III, nidogen-1, SPARC and desmin) or normal serum (biglycan) in place of the primary antibody are inset in A, B, E, F, I, J, M, N, Q and R. Black squares on A and B indicate the antimesometrial pole magnified in C and D. Abbreviations: connective tissue (ct), myometrium (my), mesometrial pole (m), antimesometrial pole (am), luminal epithelium (le), glandular epithelium (ge), decidualized stromal cell (dsc), non-decidualized stromal cell (sc), blood vessel (bv), glycocalyx (gly). Scale bar = 50 μm (A, B, E, F, I, J, M, N, Q and R are at the same magnification; C, D, G and P are at the same magnification; H, K, L, O, S, T and inset in G are at the same magnification). |
PMC535545_F4_808.jpg | Describe the main subject of this image. | Immunohistochemistry for extracellular matrix components. Immunohistochemical staining of wild type (A, C, E, G, I, K, M, O, P, Q, S) and IL11Ra-/- (B, D, F, H, J, L, N, R, T) uterus at 48 h of decidualization using specific antibodies for collagen III (A, B, C, D), biglycan (E, F, G, H), nidogen-1 (I, J, K, L), SPARC (M, N, O, P) and desmin (Q, R, S, T). Negative controls using a matching concentration of non-immune IgG (collagen III, nidogen-1, SPARC and desmin) or normal serum (biglycan) in place of the primary antibody are inset in A, B, E, F, I, J, M, N, Q and R. Black squares on A and B indicate the antimesometrial pole magnified in C and D. Abbreviations: connective tissue (ct), myometrium (my), mesometrial pole (m), antimesometrial pole (am), luminal epithelium (le), glandular epithelium (ge), decidualized stromal cell (dsc), non-decidualized stromal cell (sc), blood vessel (bv), glycocalyx (gly). Scale bar = 50 μm (A, B, E, F, I, J, M, N, Q and R are at the same magnification; C, D, G and P are at the same magnification; H, K, L, O, S, T and inset in G are at the same magnification). |
PMC535545_F4_804.jpg | What is shown in this image? | Immunohistochemistry for extracellular matrix components. Immunohistochemical staining of wild type (A, C, E, G, I, K, M, O, P, Q, S) and IL11Ra-/- (B, D, F, H, J, L, N, R, T) uterus at 48 h of decidualization using specific antibodies for collagen III (A, B, C, D), biglycan (E, F, G, H), nidogen-1 (I, J, K, L), SPARC (M, N, O, P) and desmin (Q, R, S, T). Negative controls using a matching concentration of non-immune IgG (collagen III, nidogen-1, SPARC and desmin) or normal serum (biglycan) in place of the primary antibody are inset in A, B, E, F, I, J, M, N, Q and R. Black squares on A and B indicate the antimesometrial pole magnified in C and D. Abbreviations: connective tissue (ct), myometrium (my), mesometrial pole (m), antimesometrial pole (am), luminal epithelium (le), glandular epithelium (ge), decidualized stromal cell (dsc), non-decidualized stromal cell (sc), blood vessel (bv), glycocalyx (gly). Scale bar = 50 μm (A, B, E, F, I, J, M, N, Q and R are at the same magnification; C, D, G and P are at the same magnification; H, K, L, O, S, T and inset in G are at the same magnification). |
PMC535545_F4_817.jpg | What is the main focus of this visual representation? | Immunohistochemistry for extracellular matrix components. Immunohistochemical staining of wild type (A, C, E, G, I, K, M, O, P, Q, S) and IL11Ra-/- (B, D, F, H, J, L, N, R, T) uterus at 48 h of decidualization using specific antibodies for collagen III (A, B, C, D), biglycan (E, F, G, H), nidogen-1 (I, J, K, L), SPARC (M, N, O, P) and desmin (Q, R, S, T). Negative controls using a matching concentration of non-immune IgG (collagen III, nidogen-1, SPARC and desmin) or normal serum (biglycan) in place of the primary antibody are inset in A, B, E, F, I, J, M, N, Q and R. Black squares on A and B indicate the antimesometrial pole magnified in C and D. Abbreviations: connective tissue (ct), myometrium (my), mesometrial pole (m), antimesometrial pole (am), luminal epithelium (le), glandular epithelium (ge), decidualized stromal cell (dsc), non-decidualized stromal cell (sc), blood vessel (bv), glycocalyx (gly). Scale bar = 50 μm (A, B, E, F, I, J, M, N, Q and R are at the same magnification; C, D, G and P are at the same magnification; H, K, L, O, S, T and inset in G are at the same magnification). |
PMC535545_F4_821.jpg | What is the main focus of this visual representation? | Immunohistochemistry for extracellular matrix components. Immunohistochemical staining of wild type (A, C, E, G, I, K, M, O, P, Q, S) and IL11Ra-/- (B, D, F, H, J, L, N, R, T) uterus at 48 h of decidualization using specific antibodies for collagen III (A, B, C, D), biglycan (E, F, G, H), nidogen-1 (I, J, K, L), SPARC (M, N, O, P) and desmin (Q, R, S, T). Negative controls using a matching concentration of non-immune IgG (collagen III, nidogen-1, SPARC and desmin) or normal serum (biglycan) in place of the primary antibody are inset in A, B, E, F, I, J, M, N, Q and R. Black squares on A and B indicate the antimesometrial pole magnified in C and D. Abbreviations: connective tissue (ct), myometrium (my), mesometrial pole (m), antimesometrial pole (am), luminal epithelium (le), glandular epithelium (ge), decidualized stromal cell (dsc), non-decidualized stromal cell (sc), blood vessel (bv), glycocalyx (gly). Scale bar = 50 μm (A, B, E, F, I, J, M, N, Q and R are at the same magnification; C, D, G and P are at the same magnification; H, K, L, O, S, T and inset in G are at the same magnification). |
PMC535545_F4_805.jpg | What can you see in this picture? | Immunohistochemistry for extracellular matrix components. Immunohistochemical staining of wild type (A, C, E, G, I, K, M, O, P, Q, S) and IL11Ra-/- (B, D, F, H, J, L, N, R, T) uterus at 48 h of decidualization using specific antibodies for collagen III (A, B, C, D), biglycan (E, F, G, H), nidogen-1 (I, J, K, L), SPARC (M, N, O, P) and desmin (Q, R, S, T). Negative controls using a matching concentration of non-immune IgG (collagen III, nidogen-1, SPARC and desmin) or normal serum (biglycan) in place of the primary antibody are inset in A, B, E, F, I, J, M, N, Q and R. Black squares on A and B indicate the antimesometrial pole magnified in C and D. Abbreviations: connective tissue (ct), myometrium (my), mesometrial pole (m), antimesometrial pole (am), luminal epithelium (le), glandular epithelium (ge), decidualized stromal cell (dsc), non-decidualized stromal cell (sc), blood vessel (bv), glycocalyx (gly). Scale bar = 50 μm (A, B, E, F, I, J, M, N, Q and R are at the same magnification; C, D, G and P are at the same magnification; H, K, L, O, S, T and inset in G are at the same magnification). |
PMC535545_F4_815.jpg | What key item or scene is captured in this photo? | Immunohistochemistry for extracellular matrix components. Immunohistochemical staining of wild type (A, C, E, G, I, K, M, O, P, Q, S) and IL11Ra-/- (B, D, F, H, J, L, N, R, T) uterus at 48 h of decidualization using specific antibodies for collagen III (A, B, C, D), biglycan (E, F, G, H), nidogen-1 (I, J, K, L), SPARC (M, N, O, P) and desmin (Q, R, S, T). Negative controls using a matching concentration of non-immune IgG (collagen III, nidogen-1, SPARC and desmin) or normal serum (biglycan) in place of the primary antibody are inset in A, B, E, F, I, J, M, N, Q and R. Black squares on A and B indicate the antimesometrial pole magnified in C and D. Abbreviations: connective tissue (ct), myometrium (my), mesometrial pole (m), antimesometrial pole (am), luminal epithelium (le), glandular epithelium (ge), decidualized stromal cell (dsc), non-decidualized stromal cell (sc), blood vessel (bv), glycocalyx (gly). Scale bar = 50 μm (A, B, E, F, I, J, M, N, Q and R are at the same magnification; C, D, G and P are at the same magnification; H, K, L, O, S, T and inset in G are at the same magnification). |
PMC535545_F4_811.jpg | What can you see in this picture? | Immunohistochemistry for extracellular matrix components. Immunohistochemical staining of wild type (A, C, E, G, I, K, M, O, P, Q, S) and IL11Ra-/- (B, D, F, H, J, L, N, R, T) uterus at 48 h of decidualization using specific antibodies for collagen III (A, B, C, D), biglycan (E, F, G, H), nidogen-1 (I, J, K, L), SPARC (M, N, O, P) and desmin (Q, R, S, T). Negative controls using a matching concentration of non-immune IgG (collagen III, nidogen-1, SPARC and desmin) or normal serum (biglycan) in place of the primary antibody are inset in A, B, E, F, I, J, M, N, Q and R. Black squares on A and B indicate the antimesometrial pole magnified in C and D. Abbreviations: connective tissue (ct), myometrium (my), mesometrial pole (m), antimesometrial pole (am), luminal epithelium (le), glandular epithelium (ge), decidualized stromal cell (dsc), non-decidualized stromal cell (sc), blood vessel (bv), glycocalyx (gly). Scale bar = 50 μm (A, B, E, F, I, J, M, N, Q and R are at the same magnification; C, D, G and P are at the same magnification; H, K, L, O, S, T and inset in G are at the same magnification). |
PMC535545_F4_810.jpg | What key item or scene is captured in this photo? | Immunohistochemistry for extracellular matrix components. Immunohistochemical staining of wild type (A, C, E, G, I, K, M, O, P, Q, S) and IL11Ra-/- (B, D, F, H, J, L, N, R, T) uterus at 48 h of decidualization using specific antibodies for collagen III (A, B, C, D), biglycan (E, F, G, H), nidogen-1 (I, J, K, L), SPARC (M, N, O, P) and desmin (Q, R, S, T). Negative controls using a matching concentration of non-immune IgG (collagen III, nidogen-1, SPARC and desmin) or normal serum (biglycan) in place of the primary antibody are inset in A, B, E, F, I, J, M, N, Q and R. Black squares on A and B indicate the antimesometrial pole magnified in C and D. Abbreviations: connective tissue (ct), myometrium (my), mesometrial pole (m), antimesometrial pole (am), luminal epithelium (le), glandular epithelium (ge), decidualized stromal cell (dsc), non-decidualized stromal cell (sc), blood vessel (bv), glycocalyx (gly). Scale bar = 50 μm (A, B, E, F, I, J, M, N, Q and R are at the same magnification; C, D, G and P are at the same magnification; H, K, L, O, S, T and inset in G are at the same magnification). |
PMC535550_F2_823.jpg | What is shown in this image? | 3D model of the tetrameric Fab anti-ICAM-1 molecule CFY196 [26]. Each identical subunit is represented by a different color. |
PMC535552_F2_826.jpg | Describe the main subject of this image. | Delayed chlorophyll fluorescence images. Luminescence from leaves of Arabidopsis (A) and Tradescantia (B). Images are 5-minute exposures taken as soon as possible after transfer of leaves to the equipment. A conventional photograph of the Tradescantia leaf imaged in (B) is shown to illustrate the pattern of variegation (C). |
PMC535552_F2_824.jpg | What is the focal point of this photograph? | Delayed chlorophyll fluorescence images. Luminescence from leaves of Arabidopsis (A) and Tradescantia (B). Images are 5-minute exposures taken as soon as possible after transfer of leaves to the equipment. A conventional photograph of the Tradescantia leaf imaged in (B) is shown to illustrate the pattern of variegation (C). |
PMC535552_F3_827.jpg | What is the dominant medical problem in this image? | Wound-induced bioluminescence in Arabidopsis. Sequential 5-minute exposures of wounded Arabidopsis leaves, showing strong luminescence from wound sites. "t = 5" represents an exposure from 0–5 mins after the start of the experiment; "t = 10," 5–10 mins and "t = 15," 10–15 mins. The image from t = 5 min also includes a control, unwounded leaf, which is omitted from subsequent images for brevity. |
PMC535552_F5_831.jpg | What does this image primarily show? | Chlorophyll is required for wound-induced luminescence. Luminescence imaging of leaves containing or lacking chlorophyll. The figure shows luminescence images (5 minute exposures) of wounded control (top) and photobleached, norflurazon-treated (bottom), leaves of Arabidopsis. Conventional photographs of the leaves imaged are shown at left. |
PMC535552_F6_834.jpg | What stands out most in this visual? | Temperature-dependence of luminescence. Images of the same wounded Arabidopsis leaf taken at different times following wounding, before and after the application of heat to the sample. "t = 5" represents an exposure from 0–5 mins after the start of the experiment; "t = 10," 5–10 mins and "t = 25," 20–25 mins. The heater in the lid of the sample stage was switched on after 15 mins. |
PMC535552_F6_832.jpg | What is the dominant medical problem in this image? | Temperature-dependence of luminescence. Images of the same wounded Arabidopsis leaf taken at different times following wounding, before and after the application of heat to the sample. "t = 5" represents an exposure from 0–5 mins after the start of the experiment; "t = 10," 5–10 mins and "t = 25," 20–25 mins. The heater in the lid of the sample stage was switched on after 15 mins. |
PMC535552_F7_840.jpg | What stands out most in this visual? | Heating re-activates luminescence hours after wounding. 5-minute exposures taken at different times during an experiment showing a control Arabidopsis leaf (C) and leaves wounded immediately before (0) and 15, 60 and 120 minutes before imaging. The first frame shows the luminescence image using a different display palette from the rest of the images, since it more clearly shows the strong delayed chlorophyll fluorescence emitted by all leaves over the first 5 minutes of the time course, which is absent from the dehydrated areas of the old wounds (but not the freshest wound applied immediately before imaging). All other images are displayed using the same colour palette, which is included in the figure for reference. Times indicated in the upper left corner of each panel represent the time (in minutes) relative to the start of the experiment at which each 5-minute exposure was completed. Heating began at 45 minutes. |
PMC535552_F7_837.jpg | What stands out most in this visual? | Heating re-activates luminescence hours after wounding. 5-minute exposures taken at different times during an experiment showing a control Arabidopsis leaf (C) and leaves wounded immediately before (0) and 15, 60 and 120 minutes before imaging. The first frame shows the luminescence image using a different display palette from the rest of the images, since it more clearly shows the strong delayed chlorophyll fluorescence emitted by all leaves over the first 5 minutes of the time course, which is absent from the dehydrated areas of the old wounds (but not the freshest wound applied immediately before imaging). All other images are displayed using the same colour palette, which is included in the figure for reference. Times indicated in the upper left corner of each panel represent the time (in minutes) relative to the start of the experiment at which each 5-minute exposure was completed. Heating began at 45 minutes. |
PMC535552_F7_835.jpg | Can you identify the primary element in this image? | Heating re-activates luminescence hours after wounding. 5-minute exposures taken at different times during an experiment showing a control Arabidopsis leaf (C) and leaves wounded immediately before (0) and 15, 60 and 120 minutes before imaging. The first frame shows the luminescence image using a different display palette from the rest of the images, since it more clearly shows the strong delayed chlorophyll fluorescence emitted by all leaves over the first 5 minutes of the time course, which is absent from the dehydrated areas of the old wounds (but not the freshest wound applied immediately before imaging). All other images are displayed using the same colour palette, which is included in the figure for reference. Times indicated in the upper left corner of each panel represent the time (in minutes) relative to the start of the experiment at which each 5-minute exposure was completed. Heating began at 45 minutes. |
PMC535552_F7_836.jpg | What can you see in this picture? | Heating re-activates luminescence hours after wounding. 5-minute exposures taken at different times during an experiment showing a control Arabidopsis leaf (C) and leaves wounded immediately before (0) and 15, 60 and 120 minutes before imaging. The first frame shows the luminescence image using a different display palette from the rest of the images, since it more clearly shows the strong delayed chlorophyll fluorescence emitted by all leaves over the first 5 minutes of the time course, which is absent from the dehydrated areas of the old wounds (but not the freshest wound applied immediately before imaging). All other images are displayed using the same colour palette, which is included in the figure for reference. Times indicated in the upper left corner of each panel represent the time (in minutes) relative to the start of the experiment at which each 5-minute exposure was completed. Heating began at 45 minutes. |
PMC535552_F7_838.jpg | Can you identify the primary element in this image? | Heating re-activates luminescence hours after wounding. 5-minute exposures taken at different times during an experiment showing a control Arabidopsis leaf (C) and leaves wounded immediately before (0) and 15, 60 and 120 minutes before imaging. The first frame shows the luminescence image using a different display palette from the rest of the images, since it more clearly shows the strong delayed chlorophyll fluorescence emitted by all leaves over the first 5 minutes of the time course, which is absent from the dehydrated areas of the old wounds (but not the freshest wound applied immediately before imaging). All other images are displayed using the same colour palette, which is included in the figure for reference. Times indicated in the upper left corner of each panel represent the time (in minutes) relative to the start of the experiment at which each 5-minute exposure was completed. Heating began at 45 minutes. |
PMC535552_F7_839.jpg | What is shown in this image? | Heating re-activates luminescence hours after wounding. 5-minute exposures taken at different times during an experiment showing a control Arabidopsis leaf (C) and leaves wounded immediately before (0) and 15, 60 and 120 minutes before imaging. The first frame shows the luminescence image using a different display palette from the rest of the images, since it more clearly shows the strong delayed chlorophyll fluorescence emitted by all leaves over the first 5 minutes of the time course, which is absent from the dehydrated areas of the old wounds (but not the freshest wound applied immediately before imaging). All other images are displayed using the same colour palette, which is included in the figure for reference. Times indicated in the upper left corner of each panel represent the time (in minutes) relative to the start of the experiment at which each 5-minute exposure was completed. Heating began at 45 minutes. |
PMC535567_F2_845.jpg | What is the core subject represented in this visual? | A – Chest radiograph of a patient with Pneumocystis jiroveci pneumonia showing bilateral, diffuse interstitial infiltrates B – Contrast enhanced computed tomographic (CT) scan of chest showing mediastinal lymphadenopathy in a patient with disseminated tuberculosis. Typical central necrosis evident as low attenuation areas (arrows) is seen C – Contrast enhanced CT scan of brain showing ring enhancing lesions in the basal ganglia bilaterally (arrows). Serology was positive for toxoplasma infection D – Ophthalmoscopic image of a patient with cytomegalovirus retinitis E – Non-Hodgkin's lymphoma in a HIV-infected lady presenting as unilateral maxillary swelling F – Contrast enhanced CT scan of abdomen reveals an oedematous and enlarged pancreas (asterisk) suggestive of acute pancreatitis. The patient was on didanosine and improved following withdrawal of the same and supportive treatment. |
PMC535567_F2_841.jpg | Can you identify the primary element in this image? | A – Chest radiograph of a patient with Pneumocystis jiroveci pneumonia showing bilateral, diffuse interstitial infiltrates B – Contrast enhanced computed tomographic (CT) scan of chest showing mediastinal lymphadenopathy in a patient with disseminated tuberculosis. Typical central necrosis evident as low attenuation areas (arrows) is seen C – Contrast enhanced CT scan of brain showing ring enhancing lesions in the basal ganglia bilaterally (arrows). Serology was positive for toxoplasma infection D – Ophthalmoscopic image of a patient with cytomegalovirus retinitis E – Non-Hodgkin's lymphoma in a HIV-infected lady presenting as unilateral maxillary swelling F – Contrast enhanced CT scan of abdomen reveals an oedematous and enlarged pancreas (asterisk) suggestive of acute pancreatitis. The patient was on didanosine and improved following withdrawal of the same and supportive treatment. |
PMC535567_F2_843.jpg | What key item or scene is captured in this photo? | A – Chest radiograph of a patient with Pneumocystis jiroveci pneumonia showing bilateral, diffuse interstitial infiltrates B – Contrast enhanced computed tomographic (CT) scan of chest showing mediastinal lymphadenopathy in a patient with disseminated tuberculosis. Typical central necrosis evident as low attenuation areas (arrows) is seen C – Contrast enhanced CT scan of brain showing ring enhancing lesions in the basal ganglia bilaterally (arrows). Serology was positive for toxoplasma infection D – Ophthalmoscopic image of a patient with cytomegalovirus retinitis E – Non-Hodgkin's lymphoma in a HIV-infected lady presenting as unilateral maxillary swelling F – Contrast enhanced CT scan of abdomen reveals an oedematous and enlarged pancreas (asterisk) suggestive of acute pancreatitis. The patient was on didanosine and improved following withdrawal of the same and supportive treatment. |
PMC535896_F1_848.jpg | What is the core subject represented in this visual? | Thoracic computerized tomography showing a lesion of 7 cm × 6.5 cm in diameter on the left anterolateral chest wall in the left axillary region at the level of 4.–5. ribs with minimal compressive atelectasis and destruction of the ribs. |
PMC535896_F1_847.jpg | What is the principal component of this image? | Thoracic computerized tomography showing a lesion of 7 cm × 6.5 cm in diameter on the left anterolateral chest wall in the left axillary region at the level of 4.–5. ribs with minimal compressive atelectasis and destruction of the ribs. |
PMC535927_F2_849.jpg | What is the central feature of this picture? | Screen shot of the image processing platform TIKAL. (top) Image shows a sample two-dimensional section through a nucleus with binarized nuclear particles (red) counterstained with Hoechst 33342 stain (green). Pull down menu exemplifies different tools for quantitative analysis integrated into TIKAL. Numbers indicate different nuclear particles reconstructed by 3D isosurface reconstruction (bottom). Computed tracks of nuclear bodies over time are displayed as spheres on a string in the multi-dimensional scene viewer. |
PMC538251_F1_853.jpg | What is shown in this image? | Intravascular ultrasound examples of the proximal reference site and balloon injury site 30 days after the angioplasty. Note that there was both greater intimal thickening (arrows) in the vessel treated with intravenous antisense alone, and a reduction in lumen size when compared to the proximal reference segment. The balloon injury site of the vessel treated with intravenous antisense plus PESDA and 20 kHz transcutaneous ultrasound did not exhibit any reduction in lumen area or visually evident plaque. (Reprinted with permission from Porter TR, Hiser WL, Kricsfeld D, Deligonul U, Xie F, Iversen P et al: Inhibition of carotid artery neointimal formation with intravenous microbubbles. Ultrasound Med Biol 2001, 27:259-265). |
PMC538251_F1_855.jpg | What is being portrayed in this visual content? | Intravascular ultrasound examples of the proximal reference site and balloon injury site 30 days after the angioplasty. Note that there was both greater intimal thickening (arrows) in the vessel treated with intravenous antisense alone, and a reduction in lumen size when compared to the proximal reference segment. The balloon injury site of the vessel treated with intravenous antisense plus PESDA and 20 kHz transcutaneous ultrasound did not exhibit any reduction in lumen area or visually evident plaque. (Reprinted with permission from Porter TR, Hiser WL, Kricsfeld D, Deligonul U, Xie F, Iversen P et al: Inhibition of carotid artery neointimal formation with intravenous microbubbles. Ultrasound Med Biol 2001, 27:259-265). |
PMC538251_F1_854.jpg | What can you see in this picture? | Intravascular ultrasound examples of the proximal reference site and balloon injury site 30 days after the angioplasty. Note that there was both greater intimal thickening (arrows) in the vessel treated with intravenous antisense alone, and a reduction in lumen size when compared to the proximal reference segment. The balloon injury site of the vessel treated with intravenous antisense plus PESDA and 20 kHz transcutaneous ultrasound did not exhibit any reduction in lumen area or visually evident plaque. (Reprinted with permission from Porter TR, Hiser WL, Kricsfeld D, Deligonul U, Xie F, Iversen P et al: Inhibition of carotid artery neointimal formation with intravenous microbubbles. Ultrasound Med Biol 2001, 27:259-265). |
PMC538264_F3_857.jpg | What is the central feature of this picture? | Angiotensin II receptor 1 staining in lung biopsies from control patients (A) and from patients with idiopathic pulmonary fibrosis (B). Immunohistochemistry for the angiotensin II receptor 1 (AGTR1), counterstained with haematoxylin. AGTR1 positive staining is seen in alveolar macrophages, in epithelial cells and in fibroblastic foci (arrows) in usual interstitial pneumonia biopsies (panel B). Epithelial cells and alveolar macrophages express AGTR1 in control lung biopsies (panel A). |
PMC538289_F1_858.jpg | What is the main focus of this visual representation? | Radiograph of the pelvis showing a well-circumscribed osteolytic lesion in the left iliac bone; it is sharply marginated with a thin sclerotic rim and without any matrix calcifications. |
PMC538289_F2_859.jpg | What stands out most in this visual? | a-d: MRI shows a mass originating from the ileum and extending into the soft tissues both anteromedially and posterolaterally. In T1-weighted sequences the mass is isointense with muscle (a). It enhances after administration of gadolinium (b, c). In fat-suppressed T2-weighted sequences it has high signal intensity (d). |
PMC538289_F2_860.jpg | What stands out most in this visual? | a-d: MRI shows a mass originating from the ileum and extending into the soft tissues both anteromedially and posterolaterally. In T1-weighted sequences the mass is isointense with muscle (a). It enhances after administration of gadolinium (b, c). In fat-suppressed T2-weighted sequences it has high signal intensity (d). |
PMC538289_F2_862.jpg | What key item or scene is captured in this photo? | a-d: MRI shows a mass originating from the ileum and extending into the soft tissues both anteromedially and posterolaterally. In T1-weighted sequences the mass is isointense with muscle (a). It enhances after administration of gadolinium (b, c). In fat-suppressed T2-weighted sequences it has high signal intensity (d). |
PMC538289_F7_864.jpg | What is the core subject represented in this visual? | Radiographic check 18 months after surgery, showing identical position of the fibular transplant |
PMC538289_F7_863.jpg | What stands out most in this visual? | Radiographic check 18 months after surgery, showing identical position of the fibular transplant |
PMC538290_F1_865.jpg | What is the dominant medical problem in this image? | CT scan showing a paraesophageal, retrotracheal mass, close to the thoracic inlet |
PMC538753_F2_867.jpg | What is the main focus of this visual representation? | Transient expression of GFP and GFP fusion proteins in BY-2 tobacco cells. (A) GFP protein [35SΩ-sGFP (S65T)]; (B) Arabidopsis chloroplast targeting signal (pt)-GFP fusion protein [35SΩ-pt-sGFP (S65T)]; (C) putative localization signal of GGPP synthase-GFP fusion protein [35SΩ-GGPP synthase-sGFP (S65T)] |
PMC539052_pmed-0010066-g003_870.jpg | Can you identify the primary element in this image? | Pelvic Nodal StagingNodal staging in patient with colorectal cancer. A PET scan using 18FDG as a tracer (A) and a CT scan (B) were interpreted as negative for nodal metastases. LMRI identified six small pelvic lymph nodes ([C] and [D]; red arrowheads), which had magnetic parameters of malignancy. Semiautomated reconstruction (E) identifies multisegmental metastases, subsequently proven histologically (F). For 3D reconstruction of pelvic nodal anatomy see Video 1. |
PMC539052_pmed-0010066-g003_874.jpg | What is being portrayed in this visual content? | Pelvic Nodal StagingNodal staging in patient with colorectal cancer. A PET scan using 18FDG as a tracer (A) and a CT scan (B) were interpreted as negative for nodal metastases. LMRI identified six small pelvic lymph nodes ([C] and [D]; red arrowheads), which had magnetic parameters of malignancy. Semiautomated reconstruction (E) identifies multisegmental metastases, subsequently proven histologically (F). For 3D reconstruction of pelvic nodal anatomy see Video 1. |
PMC539052_pmed-0010066-g003_871.jpg | What object or scene is depicted here? | Pelvic Nodal StagingNodal staging in patient with colorectal cancer. A PET scan using 18FDG as a tracer (A) and a CT scan (B) were interpreted as negative for nodal metastases. LMRI identified six small pelvic lymph nodes ([C] and [D]; red arrowheads), which had magnetic parameters of malignancy. Semiautomated reconstruction (E) identifies multisegmental metastases, subsequently proven histologically (F). For 3D reconstruction of pelvic nodal anatomy see Video 1. |
PMC539052_pmed-0010066-g003_869.jpg | What is the core subject represented in this visual? | Pelvic Nodal StagingNodal staging in patient with colorectal cancer. A PET scan using 18FDG as a tracer (A) and a CT scan (B) were interpreted as negative for nodal metastases. LMRI identified six small pelvic lymph nodes ([C] and [D]; red arrowheads), which had magnetic parameters of malignancy. Semiautomated reconstruction (E) identifies multisegmental metastases, subsequently proven histologically (F). For 3D reconstruction of pelvic nodal anatomy see Video 1. |
PMC539052_pmed-0010066-g003_872.jpg | What is being portrayed in this visual content? | Pelvic Nodal StagingNodal staging in patient with colorectal cancer. A PET scan using 18FDG as a tracer (A) and a CT scan (B) were interpreted as negative for nodal metastases. LMRI identified six small pelvic lymph nodes ([C] and [D]; red arrowheads), which had magnetic parameters of malignancy. Semiautomated reconstruction (E) identifies multisegmental metastases, subsequently proven histologically (F). For 3D reconstruction of pelvic nodal anatomy see Video 1. |
PMC539059_pbio-0030005-g002_876.jpg | What object or scene is depicted here? | Stereo Representations of the SARS s2m RNA Structure(A) The overall SARS s2m RNA three-dimensional structure and (B) a detailed view of tertiary contacts the and [Mg(H2O)5]2+ binding sites in the context of the experimentally phased electron density map (dark blue). The [Mg(H2O)5]2+ complex ions, depicted as white octahedra, bind to the pro-R and pro-S phosphate oxygen atoms of A(12). An extensive network of potential hydrogen bonds between the metal-coordinated water molecules and the RNA is shown as yellow dotted lines. |
PMC539059_pbio-0030005-g002_877.jpg | What key item or scene is captured in this photo? | Stereo Representations of the SARS s2m RNA Structure(A) The overall SARS s2m RNA three-dimensional structure and (B) a detailed view of tertiary contacts the and [Mg(H2O)5]2+ binding sites in the context of the experimentally phased electron density map (dark blue). The [Mg(H2O)5]2+ complex ions, depicted as white octahedra, bind to the pro-R and pro-S phosphate oxygen atoms of A(12). An extensive network of potential hydrogen bonds between the metal-coordinated water molecules and the RNA is shown as yellow dotted lines. |
PMC539059_pbio-0030005-g004_881.jpg | What does this image primarily show? | Chemical Probing of the SARS s2m RNA in Solution(A) An autoradiogram of DMS modification of the s2m RNA in solution.(B) Mapping the results of DMS, kethoxal, and CMCT modifications onto a stereo representation of the RNA structure. Red spheres represent strongly reactive N1 positions of adenosines and N3 positions of cytidine residues in the presence of DMS, and yellow spheres represent weaker reaction. Green spheres represent positions that appear to be protected from DMS. The orange sphere represents reaction with kethoxal at the N1 position of G(11), and magenta spheres represent CMCT reactions with uridines.(C) The most extensive crystal packing interaction involves stacking of G(11) upon its symmetry mate, G(11)′.(D) Temperature factors mapped onto all non-hydrogen atoms (left) and the phosphate backbone (right) of the s2m RNA crystal structure. U(25) is the most disordered residue in the structure and has the highest temperature factor. Density of the base of U(25) is not apparent even after refinement. Most of the rest of the structure is rather well ordered. |
PMC539059_pbio-0030005-g004_878.jpg | What is the central feature of this picture? | Chemical Probing of the SARS s2m RNA in Solution(A) An autoradiogram of DMS modification of the s2m RNA in solution.(B) Mapping the results of DMS, kethoxal, and CMCT modifications onto a stereo representation of the RNA structure. Red spheres represent strongly reactive N1 positions of adenosines and N3 positions of cytidine residues in the presence of DMS, and yellow spheres represent weaker reaction. Green spheres represent positions that appear to be protected from DMS. The orange sphere represents reaction with kethoxal at the N1 position of G(11), and magenta spheres represent CMCT reactions with uridines.(C) The most extensive crystal packing interaction involves stacking of G(11) upon its symmetry mate, G(11)′.(D) Temperature factors mapped onto all non-hydrogen atoms (left) and the phosphate backbone (right) of the s2m RNA crystal structure. U(25) is the most disordered residue in the structure and has the highest temperature factor. Density of the base of U(25) is not apparent even after refinement. Most of the rest of the structure is rather well ordered. |
PMC539059_pbio-0030005-g004_882.jpg | What is the principal component of this image? | Chemical Probing of the SARS s2m RNA in Solution(A) An autoradiogram of DMS modification of the s2m RNA in solution.(B) Mapping the results of DMS, kethoxal, and CMCT modifications onto a stereo representation of the RNA structure. Red spheres represent strongly reactive N1 positions of adenosines and N3 positions of cytidine residues in the presence of DMS, and yellow spheres represent weaker reaction. Green spheres represent positions that appear to be protected from DMS. The orange sphere represents reaction with kethoxal at the N1 position of G(11), and magenta spheres represent CMCT reactions with uridines.(C) The most extensive crystal packing interaction involves stacking of G(11) upon its symmetry mate, G(11)′.(D) Temperature factors mapped onto all non-hydrogen atoms (left) and the phosphate backbone (right) of the s2m RNA crystal structure. U(25) is the most disordered residue in the structure and has the highest temperature factor. Density of the base of U(25) is not apparent even after refinement. Most of the rest of the structure is rather well ordered. |
PMC539237_F1_885.jpg | What is shown in this image? | The results of group analysis. Statistical parametric maps of the average neural activity during JSL comprehension compared with rest are shown in standard anatomical space, combining hearing signers (left column), early-deaf signers (Early Deaf; second column) and late-deaf signers (Late Deaf; third column). The region of interest was confined to the temporal cortex bilaterally. The three-dimensional information was collapsed into two-dimensional sagittal and transverse images (that is, maximum-intensity projections viewed from the right and top of the brain). A direct comparison between the early- and late-deaf groups is also shown (E – L, right column). The statistical threshold is P < 0.001 (uncorrected). Right bottom, the group difference of the task-related activation (E – L) was superimposed on sagittal and coronal sections of T1-weighted high-resolution MRIs unrelated to the subjects of the present study. fMRI data were normalized in stereotaxic space. The blue lines indicate the projections of each section that cross at (-52, -22, -2). The black arrowhead indicates the STS. Bottom middle, the percent MR signal increase during JSL comprehension compared with the rest condition in the STS (-52, -22, -2) in hearing signers (H), early-deaf (E) and late-deaf signers (L). There was a significant group effect (F(2, 14) = 23.5, P < 0.001). * indicates P < 0.001, + indicates P = 0.001 (Scheffe's post hoc test). Bottom left, task-related activation in the deaf (early + late) groups. The blue lines indicate the projections of each section that cross at (-56, -26, 4). In the deaf subjects, the superior temporal cortices are extensively activated bilaterally. |
PMC539240_F2_883.jpg | What key item or scene is captured in this photo? | Computed tomography of the abdomen four years after Billroth II resection for gastric cancer, revealing an inhomogenous mass in the pancreatic head, 4 cm in diameter. (Picture courtesy the Division of Radiology, German Cancer Research Center, provided by PD Dr. med. S. Delorme). |
PMC539256_F1_889.jpg | What does this image primarily show? | Northern blot analysis with cDNA fragments enriched for leaf expressed genes. Ten micrograms total RNA from leaves (L), taproots (R), stems (S), and inflorescences (I) were hybridized with the indicated cDNA fragments (Table 1). The sizes of the hybridizing transcripts are given in kilo base pairs (kb). The RNA gels were stained prior to blotting to confirm equal loading (data not shown). |
PMC539292_F3_894.jpg | Can you identify the primary element in this image? | Increased Congo red staining of blood vessels following anti-Aβ antibody administration is associated with activated microglia. Panels A and B are from the frontal cortex of 22-month-old APP-transgenic mice immunized for 3 months with either control antibody (3A) or anti-Aβ antibody (3B). Panels C and D are from the frontal cortex of 28-month-old APP-transgenic mice immunized for 5 months with either control antibody (3C) or anti-Aβ antibody (3D). Panels E and F show a high-magnification image of CD45 immunohistochemistry (black) counterstained with Congo red (red) from 28-month-old APP-transgenic mice immunized for 5 months with either control antibody (Panel E) or anti-Aβ antibody (Panel F). Panels A-D, magnification = 100X. Scale bar in Panel B = 50 μ for panels A-D. Panels E-F, magnification = 200X. Scale bar in Panel E = 25 μm for panels E-F. |
PMC539292_F3_892.jpg | What does this image primarily show? | Increased Congo red staining of blood vessels following anti-Aβ antibody administration is associated with activated microglia. Panels A and B are from the frontal cortex of 22-month-old APP-transgenic mice immunized for 3 months with either control antibody (3A) or anti-Aβ antibody (3B). Panels C and D are from the frontal cortex of 28-month-old APP-transgenic mice immunized for 5 months with either control antibody (3C) or anti-Aβ antibody (3D). Panels E and F show a high-magnification image of CD45 immunohistochemistry (black) counterstained with Congo red (red) from 28-month-old APP-transgenic mice immunized for 5 months with either control antibody (Panel E) or anti-Aβ antibody (Panel F). Panels A-D, magnification = 100X. Scale bar in Panel B = 50 μ for panels A-D. Panels E-F, magnification = 200X. Scale bar in Panel E = 25 μm for panels E-F. |
PMC539292_F3_893.jpg | What key item or scene is captured in this photo? | Increased Congo red staining of blood vessels following anti-Aβ antibody administration is associated with activated microglia. Panels A and B are from the frontal cortex of 22-month-old APP-transgenic mice immunized for 3 months with either control antibody (3A) or anti-Aβ antibody (3B). Panels C and D are from the frontal cortex of 28-month-old APP-transgenic mice immunized for 5 months with either control antibody (3C) or anti-Aβ antibody (3D). Panels E and F show a high-magnification image of CD45 immunohistochemistry (black) counterstained with Congo red (red) from 28-month-old APP-transgenic mice immunized for 5 months with either control antibody (Panel E) or anti-Aβ antibody (Panel F). Panels A-D, magnification = 100X. Scale bar in Panel B = 50 μ for panels A-D. Panels E-F, magnification = 200X. Scale bar in Panel E = 25 μm for panels E-F. |
PMC539292_F3_895.jpg | What is shown in this image? | Increased Congo red staining of blood vessels following anti-Aβ antibody administration is associated with activated microglia. Panels A and B are from the frontal cortex of 22-month-old APP-transgenic mice immunized for 3 months with either control antibody (3A) or anti-Aβ antibody (3B). Panels C and D are from the frontal cortex of 28-month-old APP-transgenic mice immunized for 5 months with either control antibody (3C) or anti-Aβ antibody (3D). Panels E and F show a high-magnification image of CD45 immunohistochemistry (black) counterstained with Congo red (red) from 28-month-old APP-transgenic mice immunized for 5 months with either control antibody (Panel E) or anti-Aβ antibody (Panel F). Panels A-D, magnification = 100X. Scale bar in Panel B = 50 μ for panels A-D. Panels E-F, magnification = 200X. Scale bar in Panel E = 25 μm for panels E-F. |
PMC539292_F3_896.jpg | What object or scene is depicted here? | Increased Congo red staining of blood vessels following anti-Aβ antibody administration is associated with activated microglia. Panels A and B are from the frontal cortex of 22-month-old APP-transgenic mice immunized for 3 months with either control antibody (3A) or anti-Aβ antibody (3B). Panels C and D are from the frontal cortex of 28-month-old APP-transgenic mice immunized for 5 months with either control antibody (3C) or anti-Aβ antibody (3D). Panels E and F show a high-magnification image of CD45 immunohistochemistry (black) counterstained with Congo red (red) from 28-month-old APP-transgenic mice immunized for 5 months with either control antibody (Panel E) or anti-Aβ antibody (Panel F). Panels A-D, magnification = 100X. Scale bar in Panel B = 50 μ for panels A-D. Panels E-F, magnification = 200X. Scale bar in Panel E = 25 μm for panels E-F. |
PMC539297_F1_901.jpg | What does this image primarily show? | Neuronal marker expression in CbCln3+/+ cells Characterization of CbCln3+/+ cells by immunofluorescence with marker antibodies is shown. CbCln3+/+ precursors exhibit nestin expression (a) but not GFAP expression (b), consistent with a neuronal precursor identity. Upon stimulation with a differentiation cocktail (see Methods), CbCln3+/+ cells achieved neuron-like morphology, with rounded cell bodies and extension of processes, and MAP2 (c) and NeuN (d) expression was increased. CbCln3+/+ cells are negative for the Purkinje neuron marker calbindin (e). CbCln3+/Δex7/8 and CbCln3Δex7/8/Δex7/8 cell lines exhibited identical marker immunofluorescence results. a, b) 20 × magnification; c, d, e) 40 × magnification. |
PMC539297_F1_899.jpg | Can you identify the primary element in this image? | Neuronal marker expression in CbCln3+/+ cells Characterization of CbCln3+/+ cells by immunofluorescence with marker antibodies is shown. CbCln3+/+ precursors exhibit nestin expression (a) but not GFAP expression (b), consistent with a neuronal precursor identity. Upon stimulation with a differentiation cocktail (see Methods), CbCln3+/+ cells achieved neuron-like morphology, with rounded cell bodies and extension of processes, and MAP2 (c) and NeuN (d) expression was increased. CbCln3+/+ cells are negative for the Purkinje neuron marker calbindin (e). CbCln3+/Δex7/8 and CbCln3Δex7/8/Δex7/8 cell lines exhibited identical marker immunofluorescence results. a, b) 20 × magnification; c, d, e) 40 × magnification. |
PMC539297_F1_898.jpg | What is the central feature of this picture? | Neuronal marker expression in CbCln3+/+ cells Characterization of CbCln3+/+ cells by immunofluorescence with marker antibodies is shown. CbCln3+/+ precursors exhibit nestin expression (a) but not GFAP expression (b), consistent with a neuronal precursor identity. Upon stimulation with a differentiation cocktail (see Methods), CbCln3+/+ cells achieved neuron-like morphology, with rounded cell bodies and extension of processes, and MAP2 (c) and NeuN (d) expression was increased. CbCln3+/+ cells are negative for the Purkinje neuron marker calbindin (e). CbCln3+/Δex7/8 and CbCln3Δex7/8/Δex7/8 cell lines exhibited identical marker immunofluorescence results. a, b) 20 × magnification; c, d, e) 40 × magnification. |
PMC539297_F1_900.jpg | Describe the main subject of this image. | Neuronal marker expression in CbCln3+/+ cells Characterization of CbCln3+/+ cells by immunofluorescence with marker antibodies is shown. CbCln3+/+ precursors exhibit nestin expression (a) but not GFAP expression (b), consistent with a neuronal precursor identity. Upon stimulation with a differentiation cocktail (see Methods), CbCln3+/+ cells achieved neuron-like morphology, with rounded cell bodies and extension of processes, and MAP2 (c) and NeuN (d) expression was increased. CbCln3+/+ cells are negative for the Purkinje neuron marker calbindin (e). CbCln3+/Δex7/8 and CbCln3Δex7/8/Δex7/8 cell lines exhibited identical marker immunofluorescence results. a, b) 20 × magnification; c, d, e) 40 × magnification. |
PMC539297_F3_914.jpg | What is the central feature of this picture? | Battenin and lysosomal and endosomal marker co-staining in wild-type and homozygous CbCln3Δex7/8 cerebellar precursor cells Batp1 immunostaining of wild-type (CbCln3+/+) and homozygous mutant (CbCln3Δex7/8/Δex7/8) cerebellar precursor cells is shown, with co-staining for lysosomes (Lamp 1), early endosomes (EEA1), and late endosomes (Rab7). Significant overlap of Batp1 signal (red) with EEA1 (green, middle panels) and Rab7 (green, bottom panels) can be seen as yellow when the two channels are merged (Merge). The degree of Batp1 overlap is greatest with Rab7. Only limited overlap between Batp1 (red) and Lamp 1 (green, top panels) can be seen. Batp1 signal in homozygous CbCln3Δex7/8 cells is significantly reduced, but significant overlap with EEA1 and Rab7, and very little Lamp 1 overlap, can be seen as yellow in the respective merged panels. Notably, Lamp 1 and EEA1 localization appear altered, and Rab7 staining was frequently less intense in homozygous CbCln3Δex7/8 cells. Wild-type and homozygous CbCln3Δex7/8 confocal images were captured with identical exposure settings. 60 × magnification. |
PMC539297_F3_905.jpg | Can you identify the primary element in this image? | Battenin and lysosomal and endosomal marker co-staining in wild-type and homozygous CbCln3Δex7/8 cerebellar precursor cells Batp1 immunostaining of wild-type (CbCln3+/+) and homozygous mutant (CbCln3Δex7/8/Δex7/8) cerebellar precursor cells is shown, with co-staining for lysosomes (Lamp 1), early endosomes (EEA1), and late endosomes (Rab7). Significant overlap of Batp1 signal (red) with EEA1 (green, middle panels) and Rab7 (green, bottom panels) can be seen as yellow when the two channels are merged (Merge). The degree of Batp1 overlap is greatest with Rab7. Only limited overlap between Batp1 (red) and Lamp 1 (green, top panels) can be seen. Batp1 signal in homozygous CbCln3Δex7/8 cells is significantly reduced, but significant overlap with EEA1 and Rab7, and very little Lamp 1 overlap, can be seen as yellow in the respective merged panels. Notably, Lamp 1 and EEA1 localization appear altered, and Rab7 staining was frequently less intense in homozygous CbCln3Δex7/8 cells. Wild-type and homozygous CbCln3Δex7/8 confocal images were captured with identical exposure settings. 60 × magnification. |
PMC539297_F3_909.jpg | Can you identify the primary element in this image? | Battenin and lysosomal and endosomal marker co-staining in wild-type and homozygous CbCln3Δex7/8 cerebellar precursor cells Batp1 immunostaining of wild-type (CbCln3+/+) and homozygous mutant (CbCln3Δex7/8/Δex7/8) cerebellar precursor cells is shown, with co-staining for lysosomes (Lamp 1), early endosomes (EEA1), and late endosomes (Rab7). Significant overlap of Batp1 signal (red) with EEA1 (green, middle panels) and Rab7 (green, bottom panels) can be seen as yellow when the two channels are merged (Merge). The degree of Batp1 overlap is greatest with Rab7. Only limited overlap between Batp1 (red) and Lamp 1 (green, top panels) can be seen. Batp1 signal in homozygous CbCln3Δex7/8 cells is significantly reduced, but significant overlap with EEA1 and Rab7, and very little Lamp 1 overlap, can be seen as yellow in the respective merged panels. Notably, Lamp 1 and EEA1 localization appear altered, and Rab7 staining was frequently less intense in homozygous CbCln3Δex7/8 cells. Wild-type and homozygous CbCln3Δex7/8 confocal images were captured with identical exposure settings. 60 × magnification. |
PMC539297_F3_907.jpg | What is being portrayed in this visual content? | Battenin and lysosomal and endosomal marker co-staining in wild-type and homozygous CbCln3Δex7/8 cerebellar precursor cells Batp1 immunostaining of wild-type (CbCln3+/+) and homozygous mutant (CbCln3Δex7/8/Δex7/8) cerebellar precursor cells is shown, with co-staining for lysosomes (Lamp 1), early endosomes (EEA1), and late endosomes (Rab7). Significant overlap of Batp1 signal (red) with EEA1 (green, middle panels) and Rab7 (green, bottom panels) can be seen as yellow when the two channels are merged (Merge). The degree of Batp1 overlap is greatest with Rab7. Only limited overlap between Batp1 (red) and Lamp 1 (green, top panels) can be seen. Batp1 signal in homozygous CbCln3Δex7/8 cells is significantly reduced, but significant overlap with EEA1 and Rab7, and very little Lamp 1 overlap, can be seen as yellow in the respective merged panels. Notably, Lamp 1 and EEA1 localization appear altered, and Rab7 staining was frequently less intense in homozygous CbCln3Δex7/8 cells. Wild-type and homozygous CbCln3Δex7/8 confocal images were captured with identical exposure settings. 60 × magnification. |
PMC539297_F3_917.jpg | Can you identify the primary element in this image? | Battenin and lysosomal and endosomal marker co-staining in wild-type and homozygous CbCln3Δex7/8 cerebellar precursor cells Batp1 immunostaining of wild-type (CbCln3+/+) and homozygous mutant (CbCln3Δex7/8/Δex7/8) cerebellar precursor cells is shown, with co-staining for lysosomes (Lamp 1), early endosomes (EEA1), and late endosomes (Rab7). Significant overlap of Batp1 signal (red) with EEA1 (green, middle panels) and Rab7 (green, bottom panels) can be seen as yellow when the two channels are merged (Merge). The degree of Batp1 overlap is greatest with Rab7. Only limited overlap between Batp1 (red) and Lamp 1 (green, top panels) can be seen. Batp1 signal in homozygous CbCln3Δex7/8 cells is significantly reduced, but significant overlap with EEA1 and Rab7, and very little Lamp 1 overlap, can be seen as yellow in the respective merged panels. Notably, Lamp 1 and EEA1 localization appear altered, and Rab7 staining was frequently less intense in homozygous CbCln3Δex7/8 cells. Wild-type and homozygous CbCln3Δex7/8 confocal images were captured with identical exposure settings. 60 × magnification. |
PMC539297_F3_910.jpg | What is the principal component of this image? | Battenin and lysosomal and endosomal marker co-staining in wild-type and homozygous CbCln3Δex7/8 cerebellar precursor cells Batp1 immunostaining of wild-type (CbCln3+/+) and homozygous mutant (CbCln3Δex7/8/Δex7/8) cerebellar precursor cells is shown, with co-staining for lysosomes (Lamp 1), early endosomes (EEA1), and late endosomes (Rab7). Significant overlap of Batp1 signal (red) with EEA1 (green, middle panels) and Rab7 (green, bottom panels) can be seen as yellow when the two channels are merged (Merge). The degree of Batp1 overlap is greatest with Rab7. Only limited overlap between Batp1 (red) and Lamp 1 (green, top panels) can be seen. Batp1 signal in homozygous CbCln3Δex7/8 cells is significantly reduced, but significant overlap with EEA1 and Rab7, and very little Lamp 1 overlap, can be seen as yellow in the respective merged panels. Notably, Lamp 1 and EEA1 localization appear altered, and Rab7 staining was frequently less intense in homozygous CbCln3Δex7/8 cells. Wild-type and homozygous CbCln3Δex7/8 confocal images were captured with identical exposure settings. 60 × magnification. |
PMC539297_F3_913.jpg | Can you identify the primary element in this image? | Battenin and lysosomal and endosomal marker co-staining in wild-type and homozygous CbCln3Δex7/8 cerebellar precursor cells Batp1 immunostaining of wild-type (CbCln3+/+) and homozygous mutant (CbCln3Δex7/8/Δex7/8) cerebellar precursor cells is shown, with co-staining for lysosomes (Lamp 1), early endosomes (EEA1), and late endosomes (Rab7). Significant overlap of Batp1 signal (red) with EEA1 (green, middle panels) and Rab7 (green, bottom panels) can be seen as yellow when the two channels are merged (Merge). The degree of Batp1 overlap is greatest with Rab7. Only limited overlap between Batp1 (red) and Lamp 1 (green, top panels) can be seen. Batp1 signal in homozygous CbCln3Δex7/8 cells is significantly reduced, but significant overlap with EEA1 and Rab7, and very little Lamp 1 overlap, can be seen as yellow in the respective merged panels. Notably, Lamp 1 and EEA1 localization appear altered, and Rab7 staining was frequently less intense in homozygous CbCln3Δex7/8 cells. Wild-type and homozygous CbCln3Δex7/8 confocal images were captured with identical exposure settings. 60 × magnification. |
PMC539297_F3_915.jpg | What is shown in this image? | Battenin and lysosomal and endosomal marker co-staining in wild-type and homozygous CbCln3Δex7/8 cerebellar precursor cells Batp1 immunostaining of wild-type (CbCln3+/+) and homozygous mutant (CbCln3Δex7/8/Δex7/8) cerebellar precursor cells is shown, with co-staining for lysosomes (Lamp 1), early endosomes (EEA1), and late endosomes (Rab7). Significant overlap of Batp1 signal (red) with EEA1 (green, middle panels) and Rab7 (green, bottom panels) can be seen as yellow when the two channels are merged (Merge). The degree of Batp1 overlap is greatest with Rab7. Only limited overlap between Batp1 (red) and Lamp 1 (green, top panels) can be seen. Batp1 signal in homozygous CbCln3Δex7/8 cells is significantly reduced, but significant overlap with EEA1 and Rab7, and very little Lamp 1 overlap, can be seen as yellow in the respective merged panels. Notably, Lamp 1 and EEA1 localization appear altered, and Rab7 staining was frequently less intense in homozygous CbCln3Δex7/8 cells. Wild-type and homozygous CbCln3Δex7/8 confocal images were captured with identical exposure settings. 60 × magnification. |
PMC539297_F3_916.jpg | What can you see in this picture? | Battenin and lysosomal and endosomal marker co-staining in wild-type and homozygous CbCln3Δex7/8 cerebellar precursor cells Batp1 immunostaining of wild-type (CbCln3+/+) and homozygous mutant (CbCln3Δex7/8/Δex7/8) cerebellar precursor cells is shown, with co-staining for lysosomes (Lamp 1), early endosomes (EEA1), and late endosomes (Rab7). Significant overlap of Batp1 signal (red) with EEA1 (green, middle panels) and Rab7 (green, bottom panels) can be seen as yellow when the two channels are merged (Merge). The degree of Batp1 overlap is greatest with Rab7. Only limited overlap between Batp1 (red) and Lamp 1 (green, top panels) can be seen. Batp1 signal in homozygous CbCln3Δex7/8 cells is significantly reduced, but significant overlap with EEA1 and Rab7, and very little Lamp 1 overlap, can be seen as yellow in the respective merged panels. Notably, Lamp 1 and EEA1 localization appear altered, and Rab7 staining was frequently less intense in homozygous CbCln3Δex7/8 cells. Wild-type and homozygous CbCln3Δex7/8 confocal images were captured with identical exposure settings. 60 × magnification. |
PMC539297_F3_911.jpg | What key item or scene is captured in this photo? | Battenin and lysosomal and endosomal marker co-staining in wild-type and homozygous CbCln3Δex7/8 cerebellar precursor cells Batp1 immunostaining of wild-type (CbCln3+/+) and homozygous mutant (CbCln3Δex7/8/Δex7/8) cerebellar precursor cells is shown, with co-staining for lysosomes (Lamp 1), early endosomes (EEA1), and late endosomes (Rab7). Significant overlap of Batp1 signal (red) with EEA1 (green, middle panels) and Rab7 (green, bottom panels) can be seen as yellow when the two channels are merged (Merge). The degree of Batp1 overlap is greatest with Rab7. Only limited overlap between Batp1 (red) and Lamp 1 (green, top panels) can be seen. Batp1 signal in homozygous CbCln3Δex7/8 cells is significantly reduced, but significant overlap with EEA1 and Rab7, and very little Lamp 1 overlap, can be seen as yellow in the respective merged panels. Notably, Lamp 1 and EEA1 localization appear altered, and Rab7 staining was frequently less intense in homozygous CbCln3Δex7/8 cells. Wild-type and homozygous CbCln3Δex7/8 confocal images were captured with identical exposure settings. 60 × magnification. |
PMC539297_F3_906.jpg | What can you see in this picture? | Battenin and lysosomal and endosomal marker co-staining in wild-type and homozygous CbCln3Δex7/8 cerebellar precursor cells Batp1 immunostaining of wild-type (CbCln3+/+) and homozygous mutant (CbCln3Δex7/8/Δex7/8) cerebellar precursor cells is shown, with co-staining for lysosomes (Lamp 1), early endosomes (EEA1), and late endosomes (Rab7). Significant overlap of Batp1 signal (red) with EEA1 (green, middle panels) and Rab7 (green, bottom panels) can be seen as yellow when the two channels are merged (Merge). The degree of Batp1 overlap is greatest with Rab7. Only limited overlap between Batp1 (red) and Lamp 1 (green, top panels) can be seen. Batp1 signal in homozygous CbCln3Δex7/8 cells is significantly reduced, but significant overlap with EEA1 and Rab7, and very little Lamp 1 overlap, can be seen as yellow in the respective merged panels. Notably, Lamp 1 and EEA1 localization appear altered, and Rab7 staining was frequently less intense in homozygous CbCln3Δex7/8 cells. Wild-type and homozygous CbCln3Δex7/8 confocal images were captured with identical exposure settings. 60 × magnification. |
PMC539297_F3_904.jpg | What is the core subject represented in this visual? | Battenin and lysosomal and endosomal marker co-staining in wild-type and homozygous CbCln3Δex7/8 cerebellar precursor cells Batp1 immunostaining of wild-type (CbCln3+/+) and homozygous mutant (CbCln3Δex7/8/Δex7/8) cerebellar precursor cells is shown, with co-staining for lysosomes (Lamp 1), early endosomes (EEA1), and late endosomes (Rab7). Significant overlap of Batp1 signal (red) with EEA1 (green, middle panels) and Rab7 (green, bottom panels) can be seen as yellow when the two channels are merged (Merge). The degree of Batp1 overlap is greatest with Rab7. Only limited overlap between Batp1 (red) and Lamp 1 (green, top panels) can be seen. Batp1 signal in homozygous CbCln3Δex7/8 cells is significantly reduced, but significant overlap with EEA1 and Rab7, and very little Lamp 1 overlap, can be seen as yellow in the respective merged panels. Notably, Lamp 1 and EEA1 localization appear altered, and Rab7 staining was frequently less intense in homozygous CbCln3Δex7/8 cells. Wild-type and homozygous CbCln3Δex7/8 confocal images were captured with identical exposure settings. 60 × magnification. |
PMC539297_F3_903.jpg | What is the main focus of this visual representation? | Battenin and lysosomal and endosomal marker co-staining in wild-type and homozygous CbCln3Δex7/8 cerebellar precursor cells Batp1 immunostaining of wild-type (CbCln3+/+) and homozygous mutant (CbCln3Δex7/8/Δex7/8) cerebellar precursor cells is shown, with co-staining for lysosomes (Lamp 1), early endosomes (EEA1), and late endosomes (Rab7). Significant overlap of Batp1 signal (red) with EEA1 (green, middle panels) and Rab7 (green, bottom panels) can be seen as yellow when the two channels are merged (Merge). The degree of Batp1 overlap is greatest with Rab7. Only limited overlap between Batp1 (red) and Lamp 1 (green, top panels) can be seen. Batp1 signal in homozygous CbCln3Δex7/8 cells is significantly reduced, but significant overlap with EEA1 and Rab7, and very little Lamp 1 overlap, can be seen as yellow in the respective merged panels. Notably, Lamp 1 and EEA1 localization appear altered, and Rab7 staining was frequently less intense in homozygous CbCln3Δex7/8 cells. Wild-type and homozygous CbCln3Δex7/8 confocal images were captured with identical exposure settings. 60 × magnification. |
PMC539297_F3_908.jpg | Can you identify the primary element in this image? | Battenin and lysosomal and endosomal marker co-staining in wild-type and homozygous CbCln3Δex7/8 cerebellar precursor cells Batp1 immunostaining of wild-type (CbCln3+/+) and homozygous mutant (CbCln3Δex7/8/Δex7/8) cerebellar precursor cells is shown, with co-staining for lysosomes (Lamp 1), early endosomes (EEA1), and late endosomes (Rab7). Significant overlap of Batp1 signal (red) with EEA1 (green, middle panels) and Rab7 (green, bottom panels) can be seen as yellow when the two channels are merged (Merge). The degree of Batp1 overlap is greatest with Rab7. Only limited overlap between Batp1 (red) and Lamp 1 (green, top panels) can be seen. Batp1 signal in homozygous CbCln3Δex7/8 cells is significantly reduced, but significant overlap with EEA1 and Rab7, and very little Lamp 1 overlap, can be seen as yellow in the respective merged panels. Notably, Lamp 1 and EEA1 localization appear altered, and Rab7 staining was frequently less intense in homozygous CbCln3Δex7/8 cells. Wild-type and homozygous CbCln3Δex7/8 confocal images were captured with identical exposure settings. 60 × magnification. |
PMC539297_F3_918.jpg | What does this image primarily show? | Battenin and lysosomal and endosomal marker co-staining in wild-type and homozygous CbCln3Δex7/8 cerebellar precursor cells Batp1 immunostaining of wild-type (CbCln3+/+) and homozygous mutant (CbCln3Δex7/8/Δex7/8) cerebellar precursor cells is shown, with co-staining for lysosomes (Lamp 1), early endosomes (EEA1), and late endosomes (Rab7). Significant overlap of Batp1 signal (red) with EEA1 (green, middle panels) and Rab7 (green, bottom panels) can be seen as yellow when the two channels are merged (Merge). The degree of Batp1 overlap is greatest with Rab7. Only limited overlap between Batp1 (red) and Lamp 1 (green, top panels) can be seen. Batp1 signal in homozygous CbCln3Δex7/8 cells is significantly reduced, but significant overlap with EEA1 and Rab7, and very little Lamp 1 overlap, can be seen as yellow in the respective merged panels. Notably, Lamp 1 and EEA1 localization appear altered, and Rab7 staining was frequently less intense in homozygous CbCln3Δex7/8 cells. Wild-type and homozygous CbCln3Δex7/8 confocal images were captured with identical exposure settings. 60 × magnification. |
PMC539302_F2_920.jpg | Describe the main subject of this image. | Microscopic appearance of the penile lesion demonstrating a nest of atypical malignant cells (hematoxylin-eosin stain). |
PMC539305_F1_923.jpg | Can you identify the primary element in this image? | Isolation of an alveolar septum by laser-assisted microdissection and manipulation from a hemalaun stained frozen lung section (magnification 200×). A) Alveolar septum is selected for isolation. B) Laser photolysis is used to disconnect the cells from adjacent ones. C) Septum cells adhere tightly to the approximated sterile needle and can be transferred into a reaction tube. |
PMC543453_F1_927.jpg | What does this image primarily show? | Two-dimensional intracardiac echocardiography images showing part of Koch's triangle between the tricuspid valve and the ostium of the coronary sinus under four different conditions. A: Native 2D horizontal cross-sectional echocardiography image before ablation. B: The same region before ablation with use of echocontrast. C: The same region after radiofrequency energy ablation without echocontrast infusion. A crater as an indirect sign of the ablation lesion (arrow) can be seen on the endocardial surface at the atrial side adjacent to the tricuspid valve. D: The same region after radiofrequency energy ablation and during echocontrast infusion. The ablation lesion (arrow) is visualized as a low contrast area within the atrial myocardial tissue. A crater can be seen on the atrial side adjacent to the tricuspid valve. In both C and D situations (post-ablation) there is significant swelling of the ablated region compared with pre-ablation situations (A and B). ICE = central artifact of the intracardiac echocardiography catheter, TV = tricuspid valve, RA = right atrium, CSos = ostium of the coronary sinus |
PMC543453_F1_924.jpg | What is the dominant medical problem in this image? | Two-dimensional intracardiac echocardiography images showing part of Koch's triangle between the tricuspid valve and the ostium of the coronary sinus under four different conditions. A: Native 2D horizontal cross-sectional echocardiography image before ablation. B: The same region before ablation with use of echocontrast. C: The same region after radiofrequency energy ablation without echocontrast infusion. A crater as an indirect sign of the ablation lesion (arrow) can be seen on the endocardial surface at the atrial side adjacent to the tricuspid valve. D: The same region after radiofrequency energy ablation and during echocontrast infusion. The ablation lesion (arrow) is visualized as a low contrast area within the atrial myocardial tissue. A crater can be seen on the atrial side adjacent to the tricuspid valve. In both C and D situations (post-ablation) there is significant swelling of the ablated region compared with pre-ablation situations (A and B). ICE = central artifact of the intracardiac echocardiography catheter, TV = tricuspid valve, RA = right atrium, CSos = ostium of the coronary sinus |
PMC543453_F1_925.jpg | What stands out most in this visual? | Two-dimensional intracardiac echocardiography images showing part of Koch's triangle between the tricuspid valve and the ostium of the coronary sinus under four different conditions. A: Native 2D horizontal cross-sectional echocardiography image before ablation. B: The same region before ablation with use of echocontrast. C: The same region after radiofrequency energy ablation without echocontrast infusion. A crater as an indirect sign of the ablation lesion (arrow) can be seen on the endocardial surface at the atrial side adjacent to the tricuspid valve. D: The same region after radiofrequency energy ablation and during echocontrast infusion. The ablation lesion (arrow) is visualized as a low contrast area within the atrial myocardial tissue. A crater can be seen on the atrial side adjacent to the tricuspid valve. In both C and D situations (post-ablation) there is significant swelling of the ablated region compared with pre-ablation situations (A and B). ICE = central artifact of the intracardiac echocardiography catheter, TV = tricuspid valve, RA = right atrium, CSos = ostium of the coronary sinus |
PMC543453_F1_926.jpg | What key item or scene is captured in this photo? | Two-dimensional intracardiac echocardiography images showing part of Koch's triangle between the tricuspid valve and the ostium of the coronary sinus under four different conditions. A: Native 2D horizontal cross-sectional echocardiography image before ablation. B: The same region before ablation with use of echocontrast. C: The same region after radiofrequency energy ablation without echocontrast infusion. A crater as an indirect sign of the ablation lesion (arrow) can be seen on the endocardial surface at the atrial side adjacent to the tricuspid valve. D: The same region after radiofrequency energy ablation and during echocontrast infusion. The ablation lesion (arrow) is visualized as a low contrast area within the atrial myocardial tissue. A crater can be seen on the atrial side adjacent to the tricuspid valve. In both C and D situations (post-ablation) there is significant swelling of the ablated region compared with pre-ablation situations (A and B). ICE = central artifact of the intracardiac echocardiography catheter, TV = tricuspid valve, RA = right atrium, CSos = ostium of the coronary sinus |
PMC543471_F9_932.jpg | What is the principal component of this image? | Ultrasound and color Doppler examination and digital radiographs of suspected NPOA a: Axial US view combined with color Doppler of the anterior side of the left hip in a paraplegic patient presenting acute limitation and inflammation of this joint. The striation of the psoas iliaque muscle, normally detectable at the anterior part of the hip joint with US examination, has disappeared. A relatively well defined mass (orange arrows) is detectable at the anterior part of the left femoral head (F). This mass is very heterogeneous with mixed hypo and hyper echoic areas. Color Doppler enables visualization of vessels in the mass (red and blue Doppler signals). A mass effect is visible on the femoral vessels (top right of the view). b: Same patient, one week later, axial US view of the posterior side of the left hip. The classical zone phenomenon (ZP) is detectable with a central hypoechoic area surrounded by hyper echoic nodules with posterior attenuation(black arrows). c: Axial US examination at the same day combined with color Doppler view of the posterior side of the left hip. A posterior mass (orange arrows) is also visible in the gluteal muscles, very heterogeneous with mixed hypo and hyper echoic areas. Color Doppler reveals large vessels in the mass (red and blue Doppler signals). d: Plain radiographs of the left hip obtained the same day as first US examination: Any sign of ossification is visible while a well defined mass is detected by US examination. e: Plain radiographs of the left hip obtained two weeks after: Early anterior and posterior NPOA ossification is only slightly visible two weeks (Orange arrows) after the initial clinical signs whereas the US examination was initially positive. |
PMC543471_F9_928.jpg | What is the core subject represented in this visual? | Ultrasound and color Doppler examination and digital radiographs of suspected NPOA a: Axial US view combined with color Doppler of the anterior side of the left hip in a paraplegic patient presenting acute limitation and inflammation of this joint. The striation of the psoas iliaque muscle, normally detectable at the anterior part of the hip joint with US examination, has disappeared. A relatively well defined mass (orange arrows) is detectable at the anterior part of the left femoral head (F). This mass is very heterogeneous with mixed hypo and hyper echoic areas. Color Doppler enables visualization of vessels in the mass (red and blue Doppler signals). A mass effect is visible on the femoral vessels (top right of the view). b: Same patient, one week later, axial US view of the posterior side of the left hip. The classical zone phenomenon (ZP) is detectable with a central hypoechoic area surrounded by hyper echoic nodules with posterior attenuation(black arrows). c: Axial US examination at the same day combined with color Doppler view of the posterior side of the left hip. A posterior mass (orange arrows) is also visible in the gluteal muscles, very heterogeneous with mixed hypo and hyper echoic areas. Color Doppler reveals large vessels in the mass (red and blue Doppler signals). d: Plain radiographs of the left hip obtained the same day as first US examination: Any sign of ossification is visible while a well defined mass is detected by US examination. e: Plain radiographs of the left hip obtained two weeks after: Early anterior and posterior NPOA ossification is only slightly visible two weeks (Orange arrows) after the initial clinical signs whereas the US examination was initially positive. |
PMC543471_F9_929.jpg | What is the dominant medical problem in this image? | Ultrasound and color Doppler examination and digital radiographs of suspected NPOA a: Axial US view combined with color Doppler of the anterior side of the left hip in a paraplegic patient presenting acute limitation and inflammation of this joint. The striation of the psoas iliaque muscle, normally detectable at the anterior part of the hip joint with US examination, has disappeared. A relatively well defined mass (orange arrows) is detectable at the anterior part of the left femoral head (F). This mass is very heterogeneous with mixed hypo and hyper echoic areas. Color Doppler enables visualization of vessels in the mass (red and blue Doppler signals). A mass effect is visible on the femoral vessels (top right of the view). b: Same patient, one week later, axial US view of the posterior side of the left hip. The classical zone phenomenon (ZP) is detectable with a central hypoechoic area surrounded by hyper echoic nodules with posterior attenuation(black arrows). c: Axial US examination at the same day combined with color Doppler view of the posterior side of the left hip. A posterior mass (orange arrows) is also visible in the gluteal muscles, very heterogeneous with mixed hypo and hyper echoic areas. Color Doppler reveals large vessels in the mass (red and blue Doppler signals). d: Plain radiographs of the left hip obtained the same day as first US examination: Any sign of ossification is visible while a well defined mass is detected by US examination. e: Plain radiographs of the left hip obtained two weeks after: Early anterior and posterior NPOA ossification is only slightly visible two weeks (Orange arrows) after the initial clinical signs whereas the US examination was initially positive. |
PMC543471_F9_930.jpg | What can you see in this picture? | Ultrasound and color Doppler examination and digital radiographs of suspected NPOA a: Axial US view combined with color Doppler of the anterior side of the left hip in a paraplegic patient presenting acute limitation and inflammation of this joint. The striation of the psoas iliaque muscle, normally detectable at the anterior part of the hip joint with US examination, has disappeared. A relatively well defined mass (orange arrows) is detectable at the anterior part of the left femoral head (F). This mass is very heterogeneous with mixed hypo and hyper echoic areas. Color Doppler enables visualization of vessels in the mass (red and blue Doppler signals). A mass effect is visible on the femoral vessels (top right of the view). b: Same patient, one week later, axial US view of the posterior side of the left hip. The classical zone phenomenon (ZP) is detectable with a central hypoechoic area surrounded by hyper echoic nodules with posterior attenuation(black arrows). c: Axial US examination at the same day combined with color Doppler view of the posterior side of the left hip. A posterior mass (orange arrows) is also visible in the gluteal muscles, very heterogeneous with mixed hypo and hyper echoic areas. Color Doppler reveals large vessels in the mass (red and blue Doppler signals). d: Plain radiographs of the left hip obtained the same day as first US examination: Any sign of ossification is visible while a well defined mass is detected by US examination. e: Plain radiographs of the left hip obtained two weeks after: Early anterior and posterior NPOA ossification is only slightly visible two weeks (Orange arrows) after the initial clinical signs whereas the US examination was initially positive. |
PMC544194_F4_938.jpg | Describe the main subject of this image. | Example of micro CT identification of mineral composition. Visually, there appears to be only one mineral contained in the stone in the left panel and three different minerals that comprise the stone in the right panel. Micro CT attenuation identified the left stone as pure COM, and the right stone as a mixture of hydroxyapatite (bright white, highest attenuation), COM (gray) and uric acid (close to black). Speckled nature of colors – particularly apparent in image at left – is due to image noise as a result of increased magnification. |
Subsets and Splits