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PMC523228_pbio-0020352-g004_658.jpg | What is being portrayed in this visual content? | Five Newly Identified Motifs Function as Pharyngeal Enhancers(A–C) Nomarski differential contrast interference images of embryos representing three different stages of embryonic development: (A) “early” development, when the pharynx primordium is formed, (B) “mid” development, when the pharynx has completed cell division and attached to the presumptive buccal cavity, and (C) “late” development, when pharynx development is almost complete and the embryo is about to hatch. Images on the left are of “early” embryos, images in the middle are of “mid” embryos, and images on the right are of “late” embryos.(D–U) Representative transgenic embryos showing expression from reporter constructs containing the Δpes-10 promoter alone (D–F) or with insertion of three copies of Early-1 (G–I), Early-2 (J–L), Late-2 (M–O), P-1 (P–R), or P-2 (S–U). Dashed lines indicate the outline of the developing pharynx. |
PMC523228_pbio-0020352-g004_665.jpg | What does this image primarily show? | Five Newly Identified Motifs Function as Pharyngeal Enhancers(A–C) Nomarski differential contrast interference images of embryos representing three different stages of embryonic development: (A) “early” development, when the pharynx primordium is formed, (B) “mid” development, when the pharynx has completed cell division and attached to the presumptive buccal cavity, and (C) “late” development, when pharynx development is almost complete and the embryo is about to hatch. Images on the left are of “early” embryos, images in the middle are of “mid” embryos, and images on the right are of “late” embryos.(D–U) Representative transgenic embryos showing expression from reporter constructs containing the Δpes-10 promoter alone (D–F) or with insertion of three copies of Early-1 (G–I), Early-2 (J–L), Late-2 (M–O), P-1 (P–R), or P-2 (S–U). Dashed lines indicate the outline of the developing pharynx. |
PMC523228_pbio-0020352-g004_668.jpg | What is the principal component of this image? | Five Newly Identified Motifs Function as Pharyngeal Enhancers(A–C) Nomarski differential contrast interference images of embryos representing three different stages of embryonic development: (A) “early” development, when the pharynx primordium is formed, (B) “mid” development, when the pharynx has completed cell division and attached to the presumptive buccal cavity, and (C) “late” development, when pharynx development is almost complete and the embryo is about to hatch. Images on the left are of “early” embryos, images in the middle are of “mid” embryos, and images on the right are of “late” embryos.(D–U) Representative transgenic embryos showing expression from reporter constructs containing the Δpes-10 promoter alone (D–F) or with insertion of three copies of Early-1 (G–I), Early-2 (J–L), Late-2 (M–O), P-1 (P–R), or P-2 (S–U). Dashed lines indicate the outline of the developing pharynx. |
PMC523824_pmed-0010001-g001_672.jpg | What object or scene is depicted here? | Histology of Intestinal Mucosa of Two of the Oat-Intolerant PatientsSmall intestinal biopsies were obtained at diagnosis, after an ordinary gluten-free diet (remission), after introduction of oats, and after withdrawal of oats (recovery). For patient CD496, a biopsy was not taken after she started with a gluten-free diet. Biopsies were scored according to the modified Marsh criteria. Hematoxilin-eosin staining was used, and IEL counts are given in the corners of the photomicrographs. The remission biopsy from patient CD507 was poorly oriented. We therefore melted and reoriented this biopsy (insert). Original magnification: 100×. |
PMC523824_pmed-0010001-g001_676.jpg | What's the most prominent thing you notice in this picture? | Histology of Intestinal Mucosa of Two of the Oat-Intolerant PatientsSmall intestinal biopsies were obtained at diagnosis, after an ordinary gluten-free diet (remission), after introduction of oats, and after withdrawal of oats (recovery). For patient CD496, a biopsy was not taken after she started with a gluten-free diet. Biopsies were scored according to the modified Marsh criteria. Hematoxilin-eosin staining was used, and IEL counts are given in the corners of the photomicrographs. The remission biopsy from patient CD507 was poorly oriented. We therefore melted and reoriented this biopsy (insert). Original magnification: 100×. |
PMC523824_pmed-0010001-g001_677.jpg | What is the central feature of this picture? | Histology of Intestinal Mucosa of Two of the Oat-Intolerant PatientsSmall intestinal biopsies were obtained at diagnosis, after an ordinary gluten-free diet (remission), after introduction of oats, and after withdrawal of oats (recovery). For patient CD496, a biopsy was not taken after she started with a gluten-free diet. Biopsies were scored according to the modified Marsh criteria. Hematoxilin-eosin staining was used, and IEL counts are given in the corners of the photomicrographs. The remission biopsy from patient CD507 was poorly oriented. We therefore melted and reoriented this biopsy (insert). Original magnification: 100×. |
PMC523824_pmed-0010001-g001_674.jpg | What key item or scene is captured in this photo? | Histology of Intestinal Mucosa of Two of the Oat-Intolerant PatientsSmall intestinal biopsies were obtained at diagnosis, after an ordinary gluten-free diet (remission), after introduction of oats, and after withdrawal of oats (recovery). For patient CD496, a biopsy was not taken after she started with a gluten-free diet. Biopsies were scored according to the modified Marsh criteria. Hematoxilin-eosin staining was used, and IEL counts are given in the corners of the photomicrographs. The remission biopsy from patient CD507 was poorly oriented. We therefore melted and reoriented this biopsy (insert). Original magnification: 100×. |
PMC523824_pmed-0010001-g001_673.jpg | What is the main focus of this visual representation? | Histology of Intestinal Mucosa of Two of the Oat-Intolerant PatientsSmall intestinal biopsies were obtained at diagnosis, after an ordinary gluten-free diet (remission), after introduction of oats, and after withdrawal of oats (recovery). For patient CD496, a biopsy was not taken after she started with a gluten-free diet. Biopsies were scored according to the modified Marsh criteria. Hematoxilin-eosin staining was used, and IEL counts are given in the corners of the photomicrographs. The remission biopsy from patient CD507 was poorly oriented. We therefore melted and reoriented this biopsy (insert). Original magnification: 100×. |
PMC523824_pmed-0010001-g001_675.jpg | What's the most prominent thing you notice in this picture? | Histology of Intestinal Mucosa of Two of the Oat-Intolerant PatientsSmall intestinal biopsies were obtained at diagnosis, after an ordinary gluten-free diet (remission), after introduction of oats, and after withdrawal of oats (recovery). For patient CD496, a biopsy was not taken after she started with a gluten-free diet. Biopsies were scored according to the modified Marsh criteria. Hematoxilin-eosin staining was used, and IEL counts are given in the corners of the photomicrographs. The remission biopsy from patient CD507 was poorly oriented. We therefore melted and reoriented this biopsy (insert). Original magnification: 100×. |
PMC523851_F1_680.jpg | What is being portrayed in this visual content? | Analysis of the thickness of the vessel wall and the inner diameter of the vessel lumen in YP and OP. Figure A indicates YP, Figure B indicates OP. |
PMC523851_F1_679.jpg | What is the principal component of this image? | Analysis of the thickness of the vessel wall and the inner diameter of the vessel lumen in YP and OP. Figure A indicates YP, Figure B indicates OP. |
PMC524177_F2_682.jpg | What is the focal point of this photograph? | Profile generation. (A) Shows a typical region of interest (contrast enhanced for visualisation) showing the trabecular bone structure, in this case aligned approximately 22° to the vertical. (B) The central section of the FFT (128 × 128 pixels). The horizontal and vertical axes have been marked with a mid-grey tone to indicate that they have been excluded from the angle calculation. The bright strip at the centre (running from top left to bottom right) shows the preferred orientation of the trabeculae. Angles calculated from the Fourier power spectrum correspond to the same angles in the spatial domain, rotated by 90°. (C) The pixels with the maximum values are marked using white squares for the first 25 spatial frequency values of the Fourier power spectrum. The median angle, lying 21.8° from the horizontal is shown by a dashed white line. (D)_The regions used to generate the parallel (shaded black) and perpendicular (shaded white) profiles, based on the orientation of the trabecular structure. |
PMC524177_F2_681.jpg | What is the core subject represented in this visual? | Profile generation. (A) Shows a typical region of interest (contrast enhanced for visualisation) showing the trabecular bone structure, in this case aligned approximately 22° to the vertical. (B) The central section of the FFT (128 × 128 pixels). The horizontal and vertical axes have been marked with a mid-grey tone to indicate that they have been excluded from the angle calculation. The bright strip at the centre (running from top left to bottom right) shows the preferred orientation of the trabeculae. Angles calculated from the Fourier power spectrum correspond to the same angles in the spatial domain, rotated by 90°. (C) The pixels with the maximum values are marked using white squares for the first 25 spatial frequency values of the Fourier power spectrum. The median angle, lying 21.8° from the horizontal is shown by a dashed white line. (D)_The regions used to generate the parallel (shaded black) and perpendicular (shaded white) profiles, based on the orientation of the trabecular structure. |
PMC524181_F1_686.jpg | What is the main focus of this visual representation? | The Interface of ImageParser When Loading a 3D Image. The image is automatically shown slice by slice with the slice number shown in the text box, and the interval between two slices can be changed. Clicking the slide bar or text box, we can focus on the current slice; double clicking the window area, we can navigate the image slice by slice again; and dragging the slide bar or inputting the slice number in the text box, we can jump to the desired slice. |
PMC524181_F1_685.jpg | Can you identify the primary element in this image? | The Interface of ImageParser When Loading a 3D Image. The image is automatically shown slice by slice with the slice number shown in the text box, and the interval between two slices can be changed. Clicking the slide bar or text box, we can focus on the current slice; double clicking the window area, we can navigate the image slice by slice again; and dragging the slide bar or inputting the slice number in the text box, we can jump to the desired slice. |
PMC524183_F1_688.jpg | What is the core subject represented in this visual? | X-ray of pelvis showing gas in the urinary bladder wall (Arrow) |
PMC524185_F1_690.jpg | What key item or scene is captured in this photo? | Composite image showing an angiographic right anterior oblique projection of the left coronary system (Panel B) and two intravascular ultrasound images (Panels A&C) before the intervention. Dotted line shows the approximate location of the IVUS slices. An eccentric plaque is visible in the proximal left anterior descending artery (Panel B), with some haziness at the distal end. IVUS confirms the presence of the plaque (Panel A) and shows a bulky, partially organized, thrombus (Panel C) loosely attached to the distal end of the plaque. No signs of plaque rupture are visible. |
PMC524185_F1_689.jpg | What is the core subject represented in this visual? | Composite image showing an angiographic right anterior oblique projection of the left coronary system (Panel B) and two intravascular ultrasound images (Panels A&C) before the intervention. Dotted line shows the approximate location of the IVUS slices. An eccentric plaque is visible in the proximal left anterior descending artery (Panel B), with some haziness at the distal end. IVUS confirms the presence of the plaque (Panel A) and shows a bulky, partially organized, thrombus (Panel C) loosely attached to the distal end of the plaque. No signs of plaque rupture are visible. |
PMC524185_F1_691.jpg | What is being portrayed in this visual content? | Composite image showing an angiographic right anterior oblique projection of the left coronary system (Panel B) and two intravascular ultrasound images (Panels A&C) before the intervention. Dotted line shows the approximate location of the IVUS slices. An eccentric plaque is visible in the proximal left anterior descending artery (Panel B), with some haziness at the distal end. IVUS confirms the presence of the plaque (Panel A) and shows a bulky, partially organized, thrombus (Panel C) loosely attached to the distal end of the plaque. No signs of plaque rupture are visible. |
PMC524185_F1_692.jpg | What is the focal point of this photograph? | Composite image showing an angiographic right anterior oblique projection of the left coronary system (Panel B) and two intravascular ultrasound images (Panels A&C) before the intervention. Dotted line shows the approximate location of the IVUS slices. An eccentric plaque is visible in the proximal left anterior descending artery (Panel B), with some haziness at the distal end. IVUS confirms the presence of the plaque (Panel A) and shows a bulky, partially organized, thrombus (Panel C) loosely attached to the distal end of the plaque. No signs of plaque rupture are visible. |
PMC524188_F1_695.jpg | What object or scene is depicted here? | Contrast enhanced computed tomography showing the tumor location between iliocostalis and psoas muscles in retroperitoneal space. |
PMC524188_F2_693.jpg | What is the central feature of this picture? | Coronal magnetic resonance imaging demonstrating the tumor beneath the left kidney. |
PMC524188_F2_694.jpg | What is the principal component of this image? | Coronal magnetic resonance imaging demonstrating the tumor beneath the left kidney. |
PMC524251_pbio-0020367-g009_696.jpg | What stands out most in this visual? | WT Aggregates Are More Likely to Have TUNEL-Positive Cells Than ΔRD1 AggregatesRepresentative fluorescent images of aggregates following TUNEL staining of 6-d postfertilization embryos infected with 71 green-fluorescent WT (A), or 474 green-fluorescent ΔRD1 (B) bacteria. TUNEL staining is imaged with red fluorescence, and colocalization with green-fluorescent bacteria appears yellow. Scale bar, 100 μm. |
PMC524251_pbio-0020367-g009_697.jpg | What can you see in this picture? | WT Aggregates Are More Likely to Have TUNEL-Positive Cells Than ΔRD1 AggregatesRepresentative fluorescent images of aggregates following TUNEL staining of 6-d postfertilization embryos infected with 71 green-fluorescent WT (A), or 474 green-fluorescent ΔRD1 (B) bacteria. TUNEL staining is imaged with red fluorescence, and colocalization with green-fluorescent bacteria appears yellow. Scale bar, 100 μm. |
PMC524270_pbio-0020329-g001_699.jpg | What's the most prominent thing you notice in this picture? | Resolution and Contrast Using the Backscattered Electron Signal(A and B) Presynaptic vesicles (SV) and postsynaptic folds (SF) are clearly visible (A) in a motor endplate preparation embedded in Spurr's resin. Similarly, the hexagonal array of actin filaments (AA) can be clearly resolved (B) in a different region from the same image (both images were smoothed using the ImageJ “smooth” command). Imaging conditions for (A) and (B): electron energy, 7.5 keV; spot, 3.5; chamber pressure, 30 Pa (H2O); pixel dwell time, 30 μs. The scanning resolution was 6.7 nm/pixel.(C) The effect of beam exposure on the block surface. Note the increased brightness and the lack of chatter in the central region (inside the dashed rectangle), from which a stack was acquired at higher resolution before taking the image shown. The tissue was rat neocortex embedded in Spurr's resin. Imaging conditions for (C): electron energy, 7.5 keV; spot, 3; digital resolution for stack acquisition, 26.7 nm/pixel; dwell time, 30 μs.(D and E) Cortical tissue embedded in Epon. Synapses (SD) are clearly discernable (E). Imaging conditions for (D) and (E): electron energy, 7.5 keV beam current; spot, 3; chamber pressure, 30Pa (H2O); pixel dwell time, 30 μs. The scanning resolution was 9.5 nm/pixel.Note that more backscattering corresponds to darker pixels in (A), (B), (D), and (E) but to brighter pixels in (C). |
PMC524270_pbio-0020329-g001_701.jpg | What is the central feature of this picture? | Resolution and Contrast Using the Backscattered Electron Signal(A and B) Presynaptic vesicles (SV) and postsynaptic folds (SF) are clearly visible (A) in a motor endplate preparation embedded in Spurr's resin. Similarly, the hexagonal array of actin filaments (AA) can be clearly resolved (B) in a different region from the same image (both images were smoothed using the ImageJ “smooth” command). Imaging conditions for (A) and (B): electron energy, 7.5 keV; spot, 3.5; chamber pressure, 30 Pa (H2O); pixel dwell time, 30 μs. The scanning resolution was 6.7 nm/pixel.(C) The effect of beam exposure on the block surface. Note the increased brightness and the lack of chatter in the central region (inside the dashed rectangle), from which a stack was acquired at higher resolution before taking the image shown. The tissue was rat neocortex embedded in Spurr's resin. Imaging conditions for (C): electron energy, 7.5 keV; spot, 3; digital resolution for stack acquisition, 26.7 nm/pixel; dwell time, 30 μs.(D and E) Cortical tissue embedded in Epon. Synapses (SD) are clearly discernable (E). Imaging conditions for (D) and (E): electron energy, 7.5 keV beam current; spot, 3; chamber pressure, 30Pa (H2O); pixel dwell time, 30 μs. The scanning resolution was 9.5 nm/pixel.Note that more backscattering corresponds to darker pixels in (A), (B), (D), and (E) but to brighter pixels in (C). |
PMC524270_pbio-0020329-g001_700.jpg | What stands out most in this visual? | Resolution and Contrast Using the Backscattered Electron Signal(A and B) Presynaptic vesicles (SV) and postsynaptic folds (SF) are clearly visible (A) in a motor endplate preparation embedded in Spurr's resin. Similarly, the hexagonal array of actin filaments (AA) can be clearly resolved (B) in a different region from the same image (both images were smoothed using the ImageJ “smooth” command). Imaging conditions for (A) and (B): electron energy, 7.5 keV; spot, 3.5; chamber pressure, 30 Pa (H2O); pixel dwell time, 30 μs. The scanning resolution was 6.7 nm/pixel.(C) The effect of beam exposure on the block surface. Note the increased brightness and the lack of chatter in the central region (inside the dashed rectangle), from which a stack was acquired at higher resolution before taking the image shown. The tissue was rat neocortex embedded in Spurr's resin. Imaging conditions for (C): electron energy, 7.5 keV; spot, 3; digital resolution for stack acquisition, 26.7 nm/pixel; dwell time, 30 μs.(D and E) Cortical tissue embedded in Epon. Synapses (SD) are clearly discernable (E). Imaging conditions for (D) and (E): electron energy, 7.5 keV beam current; spot, 3; chamber pressure, 30Pa (H2O); pixel dwell time, 30 μs. The scanning resolution was 9.5 nm/pixel.Note that more backscattering corresponds to darker pixels in (A), (B), (D), and (E) but to brighter pixels in (C). |
PMC524360_F1_703.jpg | What object or scene is depicted here? | Sagittal MRI image of brain at first cerebral relapse demonstrating an enhancing left parasagittal lesion with surrounding cerebral oedema. |
PMC524523_F3_713.jpg | What stands out most in this visual? | Detection of HIP in human cancer specimens. By Western blotting, HIP antibodies (R&D systems Cat# AF1568) recognized one band between 75 and100 KD (A). Expression of endogenous HIP was detected in two GI cancer tissues, which were known to contain activated hedgehog signaling (data not shown here), but not in the matched normal tissue (B). Immunohistostaining of HIP I prostate cancer showed a similar pattern to PSA (C, 200×) |
PMC524523_F3_711.jpg | Can you identify the primary element in this image? | Detection of HIP in human cancer specimens. By Western blotting, HIP antibodies (R&D systems Cat# AF1568) recognized one band between 75 and100 KD (A). Expression of endogenous HIP was detected in two GI cancer tissues, which were known to contain activated hedgehog signaling (data not shown here), but not in the matched normal tissue (B). Immunohistostaining of HIP I prostate cancer showed a similar pattern to PSA (C, 200×) |
PMC524523_F4_708.jpg | Describe the main subject of this image. | Detection of Su(Fu) in prostate cancer specimens. Su(Fu) antibodies (Santa Cruz Biotechnology Cat# 10933) recognized only one single band (54-Kd) in D283 cells (A). Following treatment of a specific SiRNA of Su(Fu), the endogenous Su(Fu) band was greatly reduced (B). Immunohistostaining with Su(Fu) antibodies in prostate cancer specimens revealed positive (C, in red, 200×), negative (D, 200×) or weak staining (E, red, 200×). |
PMC524523_F4_707.jpg | What object or scene is depicted here? | Detection of Su(Fu) in prostate cancer specimens. Su(Fu) antibodies (Santa Cruz Biotechnology Cat# 10933) recognized only one single band (54-Kd) in D283 cells (A). Following treatment of a specific SiRNA of Su(Fu), the endogenous Su(Fu) band was greatly reduced (B). Immunohistostaining with Su(Fu) antibodies in prostate cancer specimens revealed positive (C, in red, 200×), negative (D, 200×) or weak staining (E, red, 200×). |
PMC526512_pbio-0030007-g006_728.jpg | What is shown in this image? | Different Elements Enhance GFP Expression in Specific Tissue and Cell TypesGFP expression is shown in fixed tissue following wholemount anti-GFP immunostaining, bright-field views (A–D, F, J, K, and N), or in live embryos as GFP fluorescence, merged bright-field and fluorescent views (E, G–I, L, M, and O). Lateral views, anterior to the left, dorsal to the top (A, B, and D–O) or dorsal view, anterior to the top (C). Embryos approximately 28–33 hpf (A, D–I, L, and O), approximately 48 hpf (B, C, J, K, and N), or approximately 26 hpf (M). The identity of the element co-injected with the GFP reporter construct is shown at the bottom of each panel. Black arrows indicate the approximate position of the midbrain–hindbrain boundary; black and white arrowheads indicate GFP-expressing cells.Scale bars approximately 100 μm (A–E, G–I, and L–O) and 50 μm (F, J, and K).b, blood island; d, diencephalon; e, eye; f, fin fold; hb, hindbrain; l, lens; n, notochord; ov, otic vesicle; r, retina; s, somite; sc, spinal cord; t, telencephalon; te, tectum; y, yolk.(A) SOX21_4. Head region (eyes removed): neurons in the telencephalon and diencephalon are GFP-positive (arrowheads).(B) SOX21_19. Head region: numerous GFP-expressing neurons are visible in the forebrain, midbrain, and hindbrain. Retinal expression is also apparent.(C) SOX21_5–6. Hindbrain region: white arrowheads indicate GFP expression by several cells in the epithelium of the right developing ear (ov). GFP-expressing cells in left deveoping ear are in slightly different focal plane.(D) SOX21_1. Trunk region: two individual notochord cells express GFP (arrowheads).(E) PAX6_6. Head region of live embryo: GFP is expressed in several retinal cells.(F) PAX6_9–10. Anterior trunk region (at the level of somites 1–3): three spinal cord neurons with ventrally projecting axons express GFP (arrowheads).(G) PAX6_1. Tail region of live embryo: arrowhead indicates GFP expression in the developing median fin fold.(H) KIAA0010_1. Trunk region, three notochord cells express GFP (arrowheads).(I) KIAA0010_2. Anterior end of embryo: arrowheads point to circulating blood cells expressing GFP.(J) HLXB9_3. Trunk region: GFP-expressing muscle fibres in somite 5 (arrowheads) lie immediately dorsal and ventral to the horizontal myoseptum.(K) HLXB9_3. Trunk region (at the level of somites 13–15): arrowheads mark GFP expression in six cells forming the epidermis or EVL.(L) SHH_6. Whole live embryo: numerous GFP-expressing muscle fibres can be seen in the trunk.(M) SHH_1. Tail region of live embryo: GFP is expressed in a single bipolar neuron near the caudal end of the spinal cord (arrowhead marks cell body).(N) SHH_4. Head region (dorsolateral view): cells labelled with anti-GFP include midbrain and hindbrain neurons and cells in the retina (slightly out of focal plane). Arrowheads indicate cell bodies of hindbrain neurons, from which axons can be seen projecting ventrally.(O) SHH_2. Trunk region of live embryo: GFP-positive cells in the region of the blood islands (caudal to the urogenital opening; arrowheads) show a slightly elongated morphology, suggesting they may be blood vessel precursors rather than blood cells. |
PMC526512_pbio-0030007-g006_716.jpg | What is being portrayed in this visual content? | Different Elements Enhance GFP Expression in Specific Tissue and Cell TypesGFP expression is shown in fixed tissue following wholemount anti-GFP immunostaining, bright-field views (A–D, F, J, K, and N), or in live embryos as GFP fluorescence, merged bright-field and fluorescent views (E, G–I, L, M, and O). Lateral views, anterior to the left, dorsal to the top (A, B, and D–O) or dorsal view, anterior to the top (C). Embryos approximately 28–33 hpf (A, D–I, L, and O), approximately 48 hpf (B, C, J, K, and N), or approximately 26 hpf (M). The identity of the element co-injected with the GFP reporter construct is shown at the bottom of each panel. Black arrows indicate the approximate position of the midbrain–hindbrain boundary; black and white arrowheads indicate GFP-expressing cells.Scale bars approximately 100 μm (A–E, G–I, and L–O) and 50 μm (F, J, and K).b, blood island; d, diencephalon; e, eye; f, fin fold; hb, hindbrain; l, lens; n, notochord; ov, otic vesicle; r, retina; s, somite; sc, spinal cord; t, telencephalon; te, tectum; y, yolk.(A) SOX21_4. Head region (eyes removed): neurons in the telencephalon and diencephalon are GFP-positive (arrowheads).(B) SOX21_19. Head region: numerous GFP-expressing neurons are visible in the forebrain, midbrain, and hindbrain. Retinal expression is also apparent.(C) SOX21_5–6. Hindbrain region: white arrowheads indicate GFP expression by several cells in the epithelium of the right developing ear (ov). GFP-expressing cells in left deveoping ear are in slightly different focal plane.(D) SOX21_1. Trunk region: two individual notochord cells express GFP (arrowheads).(E) PAX6_6. Head region of live embryo: GFP is expressed in several retinal cells.(F) PAX6_9–10. Anterior trunk region (at the level of somites 1–3): three spinal cord neurons with ventrally projecting axons express GFP (arrowheads).(G) PAX6_1. Tail region of live embryo: arrowhead indicates GFP expression in the developing median fin fold.(H) KIAA0010_1. Trunk region, three notochord cells express GFP (arrowheads).(I) KIAA0010_2. Anterior end of embryo: arrowheads point to circulating blood cells expressing GFP.(J) HLXB9_3. Trunk region: GFP-expressing muscle fibres in somite 5 (arrowheads) lie immediately dorsal and ventral to the horizontal myoseptum.(K) HLXB9_3. Trunk region (at the level of somites 13–15): arrowheads mark GFP expression in six cells forming the epidermis or EVL.(L) SHH_6. Whole live embryo: numerous GFP-expressing muscle fibres can be seen in the trunk.(M) SHH_1. Tail region of live embryo: GFP is expressed in a single bipolar neuron near the caudal end of the spinal cord (arrowhead marks cell body).(N) SHH_4. Head region (dorsolateral view): cells labelled with anti-GFP include midbrain and hindbrain neurons and cells in the retina (slightly out of focal plane). Arrowheads indicate cell bodies of hindbrain neurons, from which axons can be seen projecting ventrally.(O) SHH_2. Trunk region of live embryo: GFP-positive cells in the region of the blood islands (caudal to the urogenital opening; arrowheads) show a slightly elongated morphology, suggesting they may be blood vessel precursors rather than blood cells. |
PMC526512_pbio-0030007-g006_725.jpg | What is the dominant medical problem in this image? | Different Elements Enhance GFP Expression in Specific Tissue and Cell TypesGFP expression is shown in fixed tissue following wholemount anti-GFP immunostaining, bright-field views (A–D, F, J, K, and N), or in live embryos as GFP fluorescence, merged bright-field and fluorescent views (E, G–I, L, M, and O). Lateral views, anterior to the left, dorsal to the top (A, B, and D–O) or dorsal view, anterior to the top (C). Embryos approximately 28–33 hpf (A, D–I, L, and O), approximately 48 hpf (B, C, J, K, and N), or approximately 26 hpf (M). The identity of the element co-injected with the GFP reporter construct is shown at the bottom of each panel. Black arrows indicate the approximate position of the midbrain–hindbrain boundary; black and white arrowheads indicate GFP-expressing cells.Scale bars approximately 100 μm (A–E, G–I, and L–O) and 50 μm (F, J, and K).b, blood island; d, diencephalon; e, eye; f, fin fold; hb, hindbrain; l, lens; n, notochord; ov, otic vesicle; r, retina; s, somite; sc, spinal cord; t, telencephalon; te, tectum; y, yolk.(A) SOX21_4. Head region (eyes removed): neurons in the telencephalon and diencephalon are GFP-positive (arrowheads).(B) SOX21_19. Head region: numerous GFP-expressing neurons are visible in the forebrain, midbrain, and hindbrain. Retinal expression is also apparent.(C) SOX21_5–6. Hindbrain region: white arrowheads indicate GFP expression by several cells in the epithelium of the right developing ear (ov). GFP-expressing cells in left deveoping ear are in slightly different focal plane.(D) SOX21_1. Trunk region: two individual notochord cells express GFP (arrowheads).(E) PAX6_6. Head region of live embryo: GFP is expressed in several retinal cells.(F) PAX6_9–10. Anterior trunk region (at the level of somites 1–3): three spinal cord neurons with ventrally projecting axons express GFP (arrowheads).(G) PAX6_1. Tail region of live embryo: arrowhead indicates GFP expression in the developing median fin fold.(H) KIAA0010_1. Trunk region, three notochord cells express GFP (arrowheads).(I) KIAA0010_2. Anterior end of embryo: arrowheads point to circulating blood cells expressing GFP.(J) HLXB9_3. Trunk region: GFP-expressing muscle fibres in somite 5 (arrowheads) lie immediately dorsal and ventral to the horizontal myoseptum.(K) HLXB9_3. Trunk region (at the level of somites 13–15): arrowheads mark GFP expression in six cells forming the epidermis or EVL.(L) SHH_6. Whole live embryo: numerous GFP-expressing muscle fibres can be seen in the trunk.(M) SHH_1. Tail region of live embryo: GFP is expressed in a single bipolar neuron near the caudal end of the spinal cord (arrowhead marks cell body).(N) SHH_4. Head region (dorsolateral view): cells labelled with anti-GFP include midbrain and hindbrain neurons and cells in the retina (slightly out of focal plane). Arrowheads indicate cell bodies of hindbrain neurons, from which axons can be seen projecting ventrally.(O) SHH_2. Trunk region of live embryo: GFP-positive cells in the region of the blood islands (caudal to the urogenital opening; arrowheads) show a slightly elongated morphology, suggesting they may be blood vessel precursors rather than blood cells. |
PMC526512_pbio-0030007-g006_717.jpg | What is shown in this image? | Different Elements Enhance GFP Expression in Specific Tissue and Cell TypesGFP expression is shown in fixed tissue following wholemount anti-GFP immunostaining, bright-field views (A–D, F, J, K, and N), or in live embryos as GFP fluorescence, merged bright-field and fluorescent views (E, G–I, L, M, and O). Lateral views, anterior to the left, dorsal to the top (A, B, and D–O) or dorsal view, anterior to the top (C). Embryos approximately 28–33 hpf (A, D–I, L, and O), approximately 48 hpf (B, C, J, K, and N), or approximately 26 hpf (M). The identity of the element co-injected with the GFP reporter construct is shown at the bottom of each panel. Black arrows indicate the approximate position of the midbrain–hindbrain boundary; black and white arrowheads indicate GFP-expressing cells.Scale bars approximately 100 μm (A–E, G–I, and L–O) and 50 μm (F, J, and K).b, blood island; d, diencephalon; e, eye; f, fin fold; hb, hindbrain; l, lens; n, notochord; ov, otic vesicle; r, retina; s, somite; sc, spinal cord; t, telencephalon; te, tectum; y, yolk.(A) SOX21_4. Head region (eyes removed): neurons in the telencephalon and diencephalon are GFP-positive (arrowheads).(B) SOX21_19. Head region: numerous GFP-expressing neurons are visible in the forebrain, midbrain, and hindbrain. Retinal expression is also apparent.(C) SOX21_5–6. Hindbrain region: white arrowheads indicate GFP expression by several cells in the epithelium of the right developing ear (ov). GFP-expressing cells in left deveoping ear are in slightly different focal plane.(D) SOX21_1. Trunk region: two individual notochord cells express GFP (arrowheads).(E) PAX6_6. Head region of live embryo: GFP is expressed in several retinal cells.(F) PAX6_9–10. Anterior trunk region (at the level of somites 1–3): three spinal cord neurons with ventrally projecting axons express GFP (arrowheads).(G) PAX6_1. Tail region of live embryo: arrowhead indicates GFP expression in the developing median fin fold.(H) KIAA0010_1. Trunk region, three notochord cells express GFP (arrowheads).(I) KIAA0010_2. Anterior end of embryo: arrowheads point to circulating blood cells expressing GFP.(J) HLXB9_3. Trunk region: GFP-expressing muscle fibres in somite 5 (arrowheads) lie immediately dorsal and ventral to the horizontal myoseptum.(K) HLXB9_3. Trunk region (at the level of somites 13–15): arrowheads mark GFP expression in six cells forming the epidermis or EVL.(L) SHH_6. Whole live embryo: numerous GFP-expressing muscle fibres can be seen in the trunk.(M) SHH_1. Tail region of live embryo: GFP is expressed in a single bipolar neuron near the caudal end of the spinal cord (arrowhead marks cell body).(N) SHH_4. Head region (dorsolateral view): cells labelled with anti-GFP include midbrain and hindbrain neurons and cells in the retina (slightly out of focal plane). Arrowheads indicate cell bodies of hindbrain neurons, from which axons can be seen projecting ventrally.(O) SHH_2. Trunk region of live embryo: GFP-positive cells in the region of the blood islands (caudal to the urogenital opening; arrowheads) show a slightly elongated morphology, suggesting they may be blood vessel precursors rather than blood cells. |
PMC526512_pbio-0030007-g006_714.jpg | What is the principal component of this image? | Different Elements Enhance GFP Expression in Specific Tissue and Cell TypesGFP expression is shown in fixed tissue following wholemount anti-GFP immunostaining, bright-field views (A–D, F, J, K, and N), or in live embryos as GFP fluorescence, merged bright-field and fluorescent views (E, G–I, L, M, and O). Lateral views, anterior to the left, dorsal to the top (A, B, and D–O) or dorsal view, anterior to the top (C). Embryos approximately 28–33 hpf (A, D–I, L, and O), approximately 48 hpf (B, C, J, K, and N), or approximately 26 hpf (M). The identity of the element co-injected with the GFP reporter construct is shown at the bottom of each panel. Black arrows indicate the approximate position of the midbrain–hindbrain boundary; black and white arrowheads indicate GFP-expressing cells.Scale bars approximately 100 μm (A–E, G–I, and L–O) and 50 μm (F, J, and K).b, blood island; d, diencephalon; e, eye; f, fin fold; hb, hindbrain; l, lens; n, notochord; ov, otic vesicle; r, retina; s, somite; sc, spinal cord; t, telencephalon; te, tectum; y, yolk.(A) SOX21_4. Head region (eyes removed): neurons in the telencephalon and diencephalon are GFP-positive (arrowheads).(B) SOX21_19. Head region: numerous GFP-expressing neurons are visible in the forebrain, midbrain, and hindbrain. Retinal expression is also apparent.(C) SOX21_5–6. Hindbrain region: white arrowheads indicate GFP expression by several cells in the epithelium of the right developing ear (ov). GFP-expressing cells in left deveoping ear are in slightly different focal plane.(D) SOX21_1. Trunk region: two individual notochord cells express GFP (arrowheads).(E) PAX6_6. Head region of live embryo: GFP is expressed in several retinal cells.(F) PAX6_9–10. Anterior trunk region (at the level of somites 1–3): three spinal cord neurons with ventrally projecting axons express GFP (arrowheads).(G) PAX6_1. Tail region of live embryo: arrowhead indicates GFP expression in the developing median fin fold.(H) KIAA0010_1. Trunk region, three notochord cells express GFP (arrowheads).(I) KIAA0010_2. Anterior end of embryo: arrowheads point to circulating blood cells expressing GFP.(J) HLXB9_3. Trunk region: GFP-expressing muscle fibres in somite 5 (arrowheads) lie immediately dorsal and ventral to the horizontal myoseptum.(K) HLXB9_3. Trunk region (at the level of somites 13–15): arrowheads mark GFP expression in six cells forming the epidermis or EVL.(L) SHH_6. Whole live embryo: numerous GFP-expressing muscle fibres can be seen in the trunk.(M) SHH_1. Tail region of live embryo: GFP is expressed in a single bipolar neuron near the caudal end of the spinal cord (arrowhead marks cell body).(N) SHH_4. Head region (dorsolateral view): cells labelled with anti-GFP include midbrain and hindbrain neurons and cells in the retina (slightly out of focal plane). Arrowheads indicate cell bodies of hindbrain neurons, from which axons can be seen projecting ventrally.(O) SHH_2. Trunk region of live embryo: GFP-positive cells in the region of the blood islands (caudal to the urogenital opening; arrowheads) show a slightly elongated morphology, suggesting they may be blood vessel precursors rather than blood cells. |
PMC526512_pbio-0030007-g006_715.jpg | What is shown in this image? | Different Elements Enhance GFP Expression in Specific Tissue and Cell TypesGFP expression is shown in fixed tissue following wholemount anti-GFP immunostaining, bright-field views (A–D, F, J, K, and N), or in live embryos as GFP fluorescence, merged bright-field and fluorescent views (E, G–I, L, M, and O). Lateral views, anterior to the left, dorsal to the top (A, B, and D–O) or dorsal view, anterior to the top (C). Embryos approximately 28–33 hpf (A, D–I, L, and O), approximately 48 hpf (B, C, J, K, and N), or approximately 26 hpf (M). The identity of the element co-injected with the GFP reporter construct is shown at the bottom of each panel. Black arrows indicate the approximate position of the midbrain–hindbrain boundary; black and white arrowheads indicate GFP-expressing cells.Scale bars approximately 100 μm (A–E, G–I, and L–O) and 50 μm (F, J, and K).b, blood island; d, diencephalon; e, eye; f, fin fold; hb, hindbrain; l, lens; n, notochord; ov, otic vesicle; r, retina; s, somite; sc, spinal cord; t, telencephalon; te, tectum; y, yolk.(A) SOX21_4. Head region (eyes removed): neurons in the telencephalon and diencephalon are GFP-positive (arrowheads).(B) SOX21_19. Head region: numerous GFP-expressing neurons are visible in the forebrain, midbrain, and hindbrain. Retinal expression is also apparent.(C) SOX21_5–6. Hindbrain region: white arrowheads indicate GFP expression by several cells in the epithelium of the right developing ear (ov). GFP-expressing cells in left deveoping ear are in slightly different focal plane.(D) SOX21_1. Trunk region: two individual notochord cells express GFP (arrowheads).(E) PAX6_6. Head region of live embryo: GFP is expressed in several retinal cells.(F) PAX6_9–10. Anterior trunk region (at the level of somites 1–3): three spinal cord neurons with ventrally projecting axons express GFP (arrowheads).(G) PAX6_1. Tail region of live embryo: arrowhead indicates GFP expression in the developing median fin fold.(H) KIAA0010_1. Trunk region, three notochord cells express GFP (arrowheads).(I) KIAA0010_2. Anterior end of embryo: arrowheads point to circulating blood cells expressing GFP.(J) HLXB9_3. Trunk region: GFP-expressing muscle fibres in somite 5 (arrowheads) lie immediately dorsal and ventral to the horizontal myoseptum.(K) HLXB9_3. Trunk region (at the level of somites 13–15): arrowheads mark GFP expression in six cells forming the epidermis or EVL.(L) SHH_6. Whole live embryo: numerous GFP-expressing muscle fibres can be seen in the trunk.(M) SHH_1. Tail region of live embryo: GFP is expressed in a single bipolar neuron near the caudal end of the spinal cord (arrowhead marks cell body).(N) SHH_4. Head region (dorsolateral view): cells labelled with anti-GFP include midbrain and hindbrain neurons and cells in the retina (slightly out of focal plane). Arrowheads indicate cell bodies of hindbrain neurons, from which axons can be seen projecting ventrally.(O) SHH_2. Trunk region of live embryo: GFP-positive cells in the region of the blood islands (caudal to the urogenital opening; arrowheads) show a slightly elongated morphology, suggesting they may be blood vessel precursors rather than blood cells. |
PMC526512_pbio-0030007-g006_726.jpg | Can you identify the primary element in this image? | Different Elements Enhance GFP Expression in Specific Tissue and Cell TypesGFP expression is shown in fixed tissue following wholemount anti-GFP immunostaining, bright-field views (A–D, F, J, K, and N), or in live embryos as GFP fluorescence, merged bright-field and fluorescent views (E, G–I, L, M, and O). Lateral views, anterior to the left, dorsal to the top (A, B, and D–O) or dorsal view, anterior to the top (C). Embryos approximately 28–33 hpf (A, D–I, L, and O), approximately 48 hpf (B, C, J, K, and N), or approximately 26 hpf (M). The identity of the element co-injected with the GFP reporter construct is shown at the bottom of each panel. Black arrows indicate the approximate position of the midbrain–hindbrain boundary; black and white arrowheads indicate GFP-expressing cells.Scale bars approximately 100 μm (A–E, G–I, and L–O) and 50 μm (F, J, and K).b, blood island; d, diencephalon; e, eye; f, fin fold; hb, hindbrain; l, lens; n, notochord; ov, otic vesicle; r, retina; s, somite; sc, spinal cord; t, telencephalon; te, tectum; y, yolk.(A) SOX21_4. Head region (eyes removed): neurons in the telencephalon and diencephalon are GFP-positive (arrowheads).(B) SOX21_19. Head region: numerous GFP-expressing neurons are visible in the forebrain, midbrain, and hindbrain. Retinal expression is also apparent.(C) SOX21_5–6. Hindbrain region: white arrowheads indicate GFP expression by several cells in the epithelium of the right developing ear (ov). GFP-expressing cells in left deveoping ear are in slightly different focal plane.(D) SOX21_1. Trunk region: two individual notochord cells express GFP (arrowheads).(E) PAX6_6. Head region of live embryo: GFP is expressed in several retinal cells.(F) PAX6_9–10. Anterior trunk region (at the level of somites 1–3): three spinal cord neurons with ventrally projecting axons express GFP (arrowheads).(G) PAX6_1. Tail region of live embryo: arrowhead indicates GFP expression in the developing median fin fold.(H) KIAA0010_1. Trunk region, three notochord cells express GFP (arrowheads).(I) KIAA0010_2. Anterior end of embryo: arrowheads point to circulating blood cells expressing GFP.(J) HLXB9_3. Trunk region: GFP-expressing muscle fibres in somite 5 (arrowheads) lie immediately dorsal and ventral to the horizontal myoseptum.(K) HLXB9_3. Trunk region (at the level of somites 13–15): arrowheads mark GFP expression in six cells forming the epidermis or EVL.(L) SHH_6. Whole live embryo: numerous GFP-expressing muscle fibres can be seen in the trunk.(M) SHH_1. Tail region of live embryo: GFP is expressed in a single bipolar neuron near the caudal end of the spinal cord (arrowhead marks cell body).(N) SHH_4. Head region (dorsolateral view): cells labelled with anti-GFP include midbrain and hindbrain neurons and cells in the retina (slightly out of focal plane). Arrowheads indicate cell bodies of hindbrain neurons, from which axons can be seen projecting ventrally.(O) SHH_2. Trunk region of live embryo: GFP-positive cells in the region of the blood islands (caudal to the urogenital opening; arrowheads) show a slightly elongated morphology, suggesting they may be blood vessel precursors rather than blood cells. |
PMC526512_pbio-0030007-g006_721.jpg | What object or scene is depicted here? | Different Elements Enhance GFP Expression in Specific Tissue and Cell TypesGFP expression is shown in fixed tissue following wholemount anti-GFP immunostaining, bright-field views (A–D, F, J, K, and N), or in live embryos as GFP fluorescence, merged bright-field and fluorescent views (E, G–I, L, M, and O). Lateral views, anterior to the left, dorsal to the top (A, B, and D–O) or dorsal view, anterior to the top (C). Embryos approximately 28–33 hpf (A, D–I, L, and O), approximately 48 hpf (B, C, J, K, and N), or approximately 26 hpf (M). The identity of the element co-injected with the GFP reporter construct is shown at the bottom of each panel. Black arrows indicate the approximate position of the midbrain–hindbrain boundary; black and white arrowheads indicate GFP-expressing cells.Scale bars approximately 100 μm (A–E, G–I, and L–O) and 50 μm (F, J, and K).b, blood island; d, diencephalon; e, eye; f, fin fold; hb, hindbrain; l, lens; n, notochord; ov, otic vesicle; r, retina; s, somite; sc, spinal cord; t, telencephalon; te, tectum; y, yolk.(A) SOX21_4. Head region (eyes removed): neurons in the telencephalon and diencephalon are GFP-positive (arrowheads).(B) SOX21_19. Head region: numerous GFP-expressing neurons are visible in the forebrain, midbrain, and hindbrain. Retinal expression is also apparent.(C) SOX21_5–6. Hindbrain region: white arrowheads indicate GFP expression by several cells in the epithelium of the right developing ear (ov). GFP-expressing cells in left deveoping ear are in slightly different focal plane.(D) SOX21_1. Trunk region: two individual notochord cells express GFP (arrowheads).(E) PAX6_6. Head region of live embryo: GFP is expressed in several retinal cells.(F) PAX6_9–10. Anterior trunk region (at the level of somites 1–3): three spinal cord neurons with ventrally projecting axons express GFP (arrowheads).(G) PAX6_1. Tail region of live embryo: arrowhead indicates GFP expression in the developing median fin fold.(H) KIAA0010_1. Trunk region, three notochord cells express GFP (arrowheads).(I) KIAA0010_2. Anterior end of embryo: arrowheads point to circulating blood cells expressing GFP.(J) HLXB9_3. Trunk region: GFP-expressing muscle fibres in somite 5 (arrowheads) lie immediately dorsal and ventral to the horizontal myoseptum.(K) HLXB9_3. Trunk region (at the level of somites 13–15): arrowheads mark GFP expression in six cells forming the epidermis or EVL.(L) SHH_6. Whole live embryo: numerous GFP-expressing muscle fibres can be seen in the trunk.(M) SHH_1. Tail region of live embryo: GFP is expressed in a single bipolar neuron near the caudal end of the spinal cord (arrowhead marks cell body).(N) SHH_4. Head region (dorsolateral view): cells labelled with anti-GFP include midbrain and hindbrain neurons and cells in the retina (slightly out of focal plane). Arrowheads indicate cell bodies of hindbrain neurons, from which axons can be seen projecting ventrally.(O) SHH_2. Trunk region of live embryo: GFP-positive cells in the region of the blood islands (caudal to the urogenital opening; arrowheads) show a slightly elongated morphology, suggesting they may be blood vessel precursors rather than blood cells. |
PMC526512_pbio-0030007-g006_727.jpg | What is the main focus of this visual representation? | Different Elements Enhance GFP Expression in Specific Tissue and Cell TypesGFP expression is shown in fixed tissue following wholemount anti-GFP immunostaining, bright-field views (A–D, F, J, K, and N), or in live embryos as GFP fluorescence, merged bright-field and fluorescent views (E, G–I, L, M, and O). Lateral views, anterior to the left, dorsal to the top (A, B, and D–O) or dorsal view, anterior to the top (C). Embryos approximately 28–33 hpf (A, D–I, L, and O), approximately 48 hpf (B, C, J, K, and N), or approximately 26 hpf (M). The identity of the element co-injected with the GFP reporter construct is shown at the bottom of each panel. Black arrows indicate the approximate position of the midbrain–hindbrain boundary; black and white arrowheads indicate GFP-expressing cells.Scale bars approximately 100 μm (A–E, G–I, and L–O) and 50 μm (F, J, and K).b, blood island; d, diencephalon; e, eye; f, fin fold; hb, hindbrain; l, lens; n, notochord; ov, otic vesicle; r, retina; s, somite; sc, spinal cord; t, telencephalon; te, tectum; y, yolk.(A) SOX21_4. Head region (eyes removed): neurons in the telencephalon and diencephalon are GFP-positive (arrowheads).(B) SOX21_19. Head region: numerous GFP-expressing neurons are visible in the forebrain, midbrain, and hindbrain. Retinal expression is also apparent.(C) SOX21_5–6. Hindbrain region: white arrowheads indicate GFP expression by several cells in the epithelium of the right developing ear (ov). GFP-expressing cells in left deveoping ear are in slightly different focal plane.(D) SOX21_1. Trunk region: two individual notochord cells express GFP (arrowheads).(E) PAX6_6. Head region of live embryo: GFP is expressed in several retinal cells.(F) PAX6_9–10. Anterior trunk region (at the level of somites 1–3): three spinal cord neurons with ventrally projecting axons express GFP (arrowheads).(G) PAX6_1. Tail region of live embryo: arrowhead indicates GFP expression in the developing median fin fold.(H) KIAA0010_1. Trunk region, three notochord cells express GFP (arrowheads).(I) KIAA0010_2. Anterior end of embryo: arrowheads point to circulating blood cells expressing GFP.(J) HLXB9_3. Trunk region: GFP-expressing muscle fibres in somite 5 (arrowheads) lie immediately dorsal and ventral to the horizontal myoseptum.(K) HLXB9_3. Trunk region (at the level of somites 13–15): arrowheads mark GFP expression in six cells forming the epidermis or EVL.(L) SHH_6. Whole live embryo: numerous GFP-expressing muscle fibres can be seen in the trunk.(M) SHH_1. Tail region of live embryo: GFP is expressed in a single bipolar neuron near the caudal end of the spinal cord (arrowhead marks cell body).(N) SHH_4. Head region (dorsolateral view): cells labelled with anti-GFP include midbrain and hindbrain neurons and cells in the retina (slightly out of focal plane). Arrowheads indicate cell bodies of hindbrain neurons, from which axons can be seen projecting ventrally.(O) SHH_2. Trunk region of live embryo: GFP-positive cells in the region of the blood islands (caudal to the urogenital opening; arrowheads) show a slightly elongated morphology, suggesting they may be blood vessel precursors rather than blood cells. |
PMC526512_pbio-0030007-g006_720.jpg | What stands out most in this visual? | Different Elements Enhance GFP Expression in Specific Tissue and Cell TypesGFP expression is shown in fixed tissue following wholemount anti-GFP immunostaining, bright-field views (A–D, F, J, K, and N), or in live embryos as GFP fluorescence, merged bright-field and fluorescent views (E, G–I, L, M, and O). Lateral views, anterior to the left, dorsal to the top (A, B, and D–O) or dorsal view, anterior to the top (C). Embryos approximately 28–33 hpf (A, D–I, L, and O), approximately 48 hpf (B, C, J, K, and N), or approximately 26 hpf (M). The identity of the element co-injected with the GFP reporter construct is shown at the bottom of each panel. Black arrows indicate the approximate position of the midbrain–hindbrain boundary; black and white arrowheads indicate GFP-expressing cells.Scale bars approximately 100 μm (A–E, G–I, and L–O) and 50 μm (F, J, and K).b, blood island; d, diencephalon; e, eye; f, fin fold; hb, hindbrain; l, lens; n, notochord; ov, otic vesicle; r, retina; s, somite; sc, spinal cord; t, telencephalon; te, tectum; y, yolk.(A) SOX21_4. Head region (eyes removed): neurons in the telencephalon and diencephalon are GFP-positive (arrowheads).(B) SOX21_19. Head region: numerous GFP-expressing neurons are visible in the forebrain, midbrain, and hindbrain. Retinal expression is also apparent.(C) SOX21_5–6. Hindbrain region: white arrowheads indicate GFP expression by several cells in the epithelium of the right developing ear (ov). GFP-expressing cells in left deveoping ear are in slightly different focal plane.(D) SOX21_1. Trunk region: two individual notochord cells express GFP (arrowheads).(E) PAX6_6. Head region of live embryo: GFP is expressed in several retinal cells.(F) PAX6_9–10. Anterior trunk region (at the level of somites 1–3): three spinal cord neurons with ventrally projecting axons express GFP (arrowheads).(G) PAX6_1. Tail region of live embryo: arrowhead indicates GFP expression in the developing median fin fold.(H) KIAA0010_1. Trunk region, three notochord cells express GFP (arrowheads).(I) KIAA0010_2. Anterior end of embryo: arrowheads point to circulating blood cells expressing GFP.(J) HLXB9_3. Trunk region: GFP-expressing muscle fibres in somite 5 (arrowheads) lie immediately dorsal and ventral to the horizontal myoseptum.(K) HLXB9_3. Trunk region (at the level of somites 13–15): arrowheads mark GFP expression in six cells forming the epidermis or EVL.(L) SHH_6. Whole live embryo: numerous GFP-expressing muscle fibres can be seen in the trunk.(M) SHH_1. Tail region of live embryo: GFP is expressed in a single bipolar neuron near the caudal end of the spinal cord (arrowhead marks cell body).(N) SHH_4. Head region (dorsolateral view): cells labelled with anti-GFP include midbrain and hindbrain neurons and cells in the retina (slightly out of focal plane). Arrowheads indicate cell bodies of hindbrain neurons, from which axons can be seen projecting ventrally.(O) SHH_2. Trunk region of live embryo: GFP-positive cells in the region of the blood islands (caudal to the urogenital opening; arrowheads) show a slightly elongated morphology, suggesting they may be blood vessel precursors rather than blood cells. |
PMC526512_pbio-0030007-g006_718.jpg | What is the main focus of this visual representation? | Different Elements Enhance GFP Expression in Specific Tissue and Cell TypesGFP expression is shown in fixed tissue following wholemount anti-GFP immunostaining, bright-field views (A–D, F, J, K, and N), or in live embryos as GFP fluorescence, merged bright-field and fluorescent views (E, G–I, L, M, and O). Lateral views, anterior to the left, dorsal to the top (A, B, and D–O) or dorsal view, anterior to the top (C). Embryos approximately 28–33 hpf (A, D–I, L, and O), approximately 48 hpf (B, C, J, K, and N), or approximately 26 hpf (M). The identity of the element co-injected with the GFP reporter construct is shown at the bottom of each panel. Black arrows indicate the approximate position of the midbrain–hindbrain boundary; black and white arrowheads indicate GFP-expressing cells.Scale bars approximately 100 μm (A–E, G–I, and L–O) and 50 μm (F, J, and K).b, blood island; d, diencephalon; e, eye; f, fin fold; hb, hindbrain; l, lens; n, notochord; ov, otic vesicle; r, retina; s, somite; sc, spinal cord; t, telencephalon; te, tectum; y, yolk.(A) SOX21_4. Head region (eyes removed): neurons in the telencephalon and diencephalon are GFP-positive (arrowheads).(B) SOX21_19. Head region: numerous GFP-expressing neurons are visible in the forebrain, midbrain, and hindbrain. Retinal expression is also apparent.(C) SOX21_5–6. Hindbrain region: white arrowheads indicate GFP expression by several cells in the epithelium of the right developing ear (ov). GFP-expressing cells in left deveoping ear are in slightly different focal plane.(D) SOX21_1. Trunk region: two individual notochord cells express GFP (arrowheads).(E) PAX6_6. Head region of live embryo: GFP is expressed in several retinal cells.(F) PAX6_9–10. Anterior trunk region (at the level of somites 1–3): three spinal cord neurons with ventrally projecting axons express GFP (arrowheads).(G) PAX6_1. Tail region of live embryo: arrowhead indicates GFP expression in the developing median fin fold.(H) KIAA0010_1. Trunk region, three notochord cells express GFP (arrowheads).(I) KIAA0010_2. Anterior end of embryo: arrowheads point to circulating blood cells expressing GFP.(J) HLXB9_3. Trunk region: GFP-expressing muscle fibres in somite 5 (arrowheads) lie immediately dorsal and ventral to the horizontal myoseptum.(K) HLXB9_3. Trunk region (at the level of somites 13–15): arrowheads mark GFP expression in six cells forming the epidermis or EVL.(L) SHH_6. Whole live embryo: numerous GFP-expressing muscle fibres can be seen in the trunk.(M) SHH_1. Tail region of live embryo: GFP is expressed in a single bipolar neuron near the caudal end of the spinal cord (arrowhead marks cell body).(N) SHH_4. Head region (dorsolateral view): cells labelled with anti-GFP include midbrain and hindbrain neurons and cells in the retina (slightly out of focal plane). Arrowheads indicate cell bodies of hindbrain neurons, from which axons can be seen projecting ventrally.(O) SHH_2. Trunk region of live embryo: GFP-positive cells in the region of the blood islands (caudal to the urogenital opening; arrowheads) show a slightly elongated morphology, suggesting they may be blood vessel precursors rather than blood cells. |
PMC526512_pbio-0030007-g006_719.jpg | What key item or scene is captured in this photo? | Different Elements Enhance GFP Expression in Specific Tissue and Cell TypesGFP expression is shown in fixed tissue following wholemount anti-GFP immunostaining, bright-field views (A–D, F, J, K, and N), or in live embryos as GFP fluorescence, merged bright-field and fluorescent views (E, G–I, L, M, and O). Lateral views, anterior to the left, dorsal to the top (A, B, and D–O) or dorsal view, anterior to the top (C). Embryos approximately 28–33 hpf (A, D–I, L, and O), approximately 48 hpf (B, C, J, K, and N), or approximately 26 hpf (M). The identity of the element co-injected with the GFP reporter construct is shown at the bottom of each panel. Black arrows indicate the approximate position of the midbrain–hindbrain boundary; black and white arrowheads indicate GFP-expressing cells.Scale bars approximately 100 μm (A–E, G–I, and L–O) and 50 μm (F, J, and K).b, blood island; d, diencephalon; e, eye; f, fin fold; hb, hindbrain; l, lens; n, notochord; ov, otic vesicle; r, retina; s, somite; sc, spinal cord; t, telencephalon; te, tectum; y, yolk.(A) SOX21_4. Head region (eyes removed): neurons in the telencephalon and diencephalon are GFP-positive (arrowheads).(B) SOX21_19. Head region: numerous GFP-expressing neurons are visible in the forebrain, midbrain, and hindbrain. Retinal expression is also apparent.(C) SOX21_5–6. Hindbrain region: white arrowheads indicate GFP expression by several cells in the epithelium of the right developing ear (ov). GFP-expressing cells in left deveoping ear are in slightly different focal plane.(D) SOX21_1. Trunk region: two individual notochord cells express GFP (arrowheads).(E) PAX6_6. Head region of live embryo: GFP is expressed in several retinal cells.(F) PAX6_9–10. Anterior trunk region (at the level of somites 1–3): three spinal cord neurons with ventrally projecting axons express GFP (arrowheads).(G) PAX6_1. Tail region of live embryo: arrowhead indicates GFP expression in the developing median fin fold.(H) KIAA0010_1. Trunk region, three notochord cells express GFP (arrowheads).(I) KIAA0010_2. Anterior end of embryo: arrowheads point to circulating blood cells expressing GFP.(J) HLXB9_3. Trunk region: GFP-expressing muscle fibres in somite 5 (arrowheads) lie immediately dorsal and ventral to the horizontal myoseptum.(K) HLXB9_3. Trunk region (at the level of somites 13–15): arrowheads mark GFP expression in six cells forming the epidermis or EVL.(L) SHH_6. Whole live embryo: numerous GFP-expressing muscle fibres can be seen in the trunk.(M) SHH_1. Tail region of live embryo: GFP is expressed in a single bipolar neuron near the caudal end of the spinal cord (arrowhead marks cell body).(N) SHH_4. Head region (dorsolateral view): cells labelled with anti-GFP include midbrain and hindbrain neurons and cells in the retina (slightly out of focal plane). Arrowheads indicate cell bodies of hindbrain neurons, from which axons can be seen projecting ventrally.(O) SHH_2. Trunk region of live embryo: GFP-positive cells in the region of the blood islands (caudal to the urogenital opening; arrowheads) show a slightly elongated morphology, suggesting they may be blood vessel precursors rather than blood cells. |
PMC526771_F2_731.jpg | Describe the main subject of this image. | a) Chest CT scan displays a polypoid mass occupying 50 % of the lumen. b) Control CT scan displays resolution of the tumor |
PMC526771_F3_729.jpg | What is the dominant medical problem in this image? | Bronchoscopic examination reveals polypoid mass originating from trachea with a 50 % obstruction of the lumen. |
PMC527874_F1_732.jpg | What is the core subject represented in this visual? | Ultrasonographic appearance of the cervical mass showing it to be deceptively circumscribed |
PMC528723_pbio-0020379-g004_735.jpg | What is being portrayed in this visual content? | Kc167 Cells Exposed to dsRNA Targeting Act5C or to Cytochalasin DThe cells were exposed to dsRNA targeting Act5C for 4 d (A) or to cytochalasin D at 5 μM for 48 h (B). Tubulin is shown in red, DNA in green. |
PMC528723_pbio-0020379-g004_734.jpg | What is shown in this image? | Kc167 Cells Exposed to dsRNA Targeting Act5C or to Cytochalasin DThe cells were exposed to dsRNA targeting Act5C for 4 d (A) or to cytochalasin D at 5 μM for 48 h (B). Tubulin is shown in red, DNA in green. |
PMC529257_F1_739.jpg | What is the principal component of this image? | Radiological improvement with IFN gamma treatment (ray-x of two patients are shown). Patient 4 (A), left-lung fibroexudative lesions, and (B) complete resolution after IFN gamma treatment. Patient 2 (C), bilateral moderate exudative lesions before IFN gamma treatment, and (D) important improvement of the lesions afterwards. |
PMC529257_F1_738.jpg | What can you see in this picture? | Radiological improvement with IFN gamma treatment (ray-x of two patients are shown). Patient 4 (A), left-lung fibroexudative lesions, and (B) complete resolution after IFN gamma treatment. Patient 2 (C), bilateral moderate exudative lesions before IFN gamma treatment, and (D) important improvement of the lesions afterwards. |
PMC529257_F1_736.jpg | What stands out most in this visual? | Radiological improvement with IFN gamma treatment (ray-x of two patients are shown). Patient 4 (A), left-lung fibroexudative lesions, and (B) complete resolution after IFN gamma treatment. Patient 2 (C), bilateral moderate exudative lesions before IFN gamma treatment, and (D) important improvement of the lesions afterwards. |
PMC529257_F1_737.jpg | What does this image primarily show? | Radiological improvement with IFN gamma treatment (ray-x of two patients are shown). Patient 4 (A), left-lung fibroexudative lesions, and (B) complete resolution after IFN gamma treatment. Patient 2 (C), bilateral moderate exudative lesions before IFN gamma treatment, and (D) important improvement of the lesions afterwards. |
PMC529274_F10_742.jpg | What is the main focus of this visual representation? | Plot of Fisher score values for PD1 of the KPCA algorithm with varying bandwidth. The score indicates a varying magnitude of separation between the class of suspicious tissue signals and the class of normal tissue signals. Below, the fusion image I1 for S1 based KPCA with four different bandwidth values A, B, C and D is shown. Variation of the bandwidth leads to fusion images with varying imaging properties. The bandwidth B leads to a fusion image that displays the tumour with the highest contrast to the surrounding tissue and the Fisher score shows a peak at the corresponding position. For bandwidth values A, C and D, the Fisher score and the contrast in the fusion images decreases. |
PMC529274_F10_743.jpg | What is being portrayed in this visual content? | Plot of Fisher score values for PD1 of the KPCA algorithm with varying bandwidth. The score indicates a varying magnitude of separation between the class of suspicious tissue signals and the class of normal tissue signals. Below, the fusion image I1 for S1 based KPCA with four different bandwidth values A, B, C and D is shown. Variation of the bandwidth leads to fusion images with varying imaging properties. The bandwidth B leads to a fusion image that displays the tumour with the highest contrast to the surrounding tissue and the Fisher score shows a peak at the corresponding position. For bandwidth values A, C and D, the Fisher score and the contrast in the fusion images decreases. |
PMC529274_F10_744.jpg | What is the principal component of this image? | Plot of Fisher score values for PD1 of the KPCA algorithm with varying bandwidth. The score indicates a varying magnitude of separation between the class of suspicious tissue signals and the class of normal tissue signals. Below, the fusion image I1 for S1 based KPCA with four different bandwidth values A, B, C and D is shown. Variation of the bandwidth leads to fusion images with varying imaging properties. The bandwidth B leads to a fusion image that displays the tumour with the highest contrast to the surrounding tissue and the Fisher score shows a peak at the corresponding position. For bandwidth values A, C and D, the Fisher score and the contrast in the fusion images decreases. |
PMC529310_F1_749.jpg | What key item or scene is captured in this photo? | Chest CT before treatment (27-Sep-2001) show that conglomeration of a size of 5.5 × 4.2 cm at the left lower hilus pulmonis, large amount of accumulation of fluid in the left thoracic cavity, enlarged lymph nodes in the mediastinum. |
PMC529310_F2_745.jpg | What is the principal component of this image? | Chest CT after treatment (16-Nov-2001) show that clear shrinkage of conglomeration of 3.0 × 2.5 cm at the left lower hilus pulmonis, small amount of accumulation of fluid in the left thoracic cavity. |
PMC529310_F2_748.jpg | What is the focal point of this photograph? | Chest CT after treatment (16-Nov-2001) show that clear shrinkage of conglomeration of 3.0 × 2.5 cm at the left lower hilus pulmonis, small amount of accumulation of fluid in the left thoracic cavity. |
PMC529315_pbio-0020403-g006_754.jpg | What's the most prominent thing you notice in this picture? | Chemoattraction Towards NT-3 Beads Placed in E13 Spinal Cord DRG Explant Co-Cultures(A) NT-3 bead placed in the midline of E13 WT spinal cord. Notice axons labeled through the DRGs (circled with black dashed lines) growing towards the bead (circled with white dashed lines) enter the spinal cord at ectopic loci instead of dorsal spinal cord.(B) PBS-loaded bead in E13 spinal cord. All labeled axons extend along the dorsal spinal cord, where they terminate.(C) High-power image of the bead in (A). Notice labeled axons surrounding the bead.(D) High-power image of an NT-3-loaded bead. Notice axons bundled around the bead.(E) High-power image of an NT-3-loaded bead. Notice the axons approaching the bead via the dorsal spinal cord.(F) High-power image of a PBS-loaded bead. No labeled fibers were observed around control beads.(G) Summary of our observations from E13 spinal cord DRG organotypic cultures. In control cultures fibers extend along the dorsal spinal cord, where they normally enter the gray matter at E13. In the presence of an ectopic NT-3 source localized at the midline, these axons grow towards the NT-3 bead. NT-3 also initiates axon growth from the DRGs, entering the spinal cord at ectopic lateral loci, growing towards the bead, surrounding the bead, forming nerve bundles, and branching around it.Scale bar, 175 μm (A and B), 100 μm (C–F). |
PMC529315_pbio-0020403-g006_753.jpg | What is the focal point of this photograph? | Chemoattraction Towards NT-3 Beads Placed in E13 Spinal Cord DRG Explant Co-Cultures(A) NT-3 bead placed in the midline of E13 WT spinal cord. Notice axons labeled through the DRGs (circled with black dashed lines) growing towards the bead (circled with white dashed lines) enter the spinal cord at ectopic loci instead of dorsal spinal cord.(B) PBS-loaded bead in E13 spinal cord. All labeled axons extend along the dorsal spinal cord, where they terminate.(C) High-power image of the bead in (A). Notice labeled axons surrounding the bead.(D) High-power image of an NT-3-loaded bead. Notice axons bundled around the bead.(E) High-power image of an NT-3-loaded bead. Notice the axons approaching the bead via the dorsal spinal cord.(F) High-power image of a PBS-loaded bead. No labeled fibers were observed around control beads.(G) Summary of our observations from E13 spinal cord DRG organotypic cultures. In control cultures fibers extend along the dorsal spinal cord, where they normally enter the gray matter at E13. In the presence of an ectopic NT-3 source localized at the midline, these axons grow towards the NT-3 bead. NT-3 also initiates axon growth from the DRGs, entering the spinal cord at ectopic lateral loci, growing towards the bead, surrounding the bead, forming nerve bundles, and branching around it.Scale bar, 175 μm (A and B), 100 μm (C–F). |
PMC529315_pbio-0020403-g006_751.jpg | What does this image primarily show? | Chemoattraction Towards NT-3 Beads Placed in E13 Spinal Cord DRG Explant Co-Cultures(A) NT-3 bead placed in the midline of E13 WT spinal cord. Notice axons labeled through the DRGs (circled with black dashed lines) growing towards the bead (circled with white dashed lines) enter the spinal cord at ectopic loci instead of dorsal spinal cord.(B) PBS-loaded bead in E13 spinal cord. All labeled axons extend along the dorsal spinal cord, where they terminate.(C) High-power image of the bead in (A). Notice labeled axons surrounding the bead.(D) High-power image of an NT-3-loaded bead. Notice axons bundled around the bead.(E) High-power image of an NT-3-loaded bead. Notice the axons approaching the bead via the dorsal spinal cord.(F) High-power image of a PBS-loaded bead. No labeled fibers were observed around control beads.(G) Summary of our observations from E13 spinal cord DRG organotypic cultures. In control cultures fibers extend along the dorsal spinal cord, where they normally enter the gray matter at E13. In the presence of an ectopic NT-3 source localized at the midline, these axons grow towards the NT-3 bead. NT-3 also initiates axon growth from the DRGs, entering the spinal cord at ectopic lateral loci, growing towards the bead, surrounding the bead, forming nerve bundles, and branching around it.Scale bar, 175 μm (A and B), 100 μm (C–F). |
PMC529315_pbio-0020403-g006_756.jpg | What is being portrayed in this visual content? | Chemoattraction Towards NT-3 Beads Placed in E13 Spinal Cord DRG Explant Co-Cultures(A) NT-3 bead placed in the midline of E13 WT spinal cord. Notice axons labeled through the DRGs (circled with black dashed lines) growing towards the bead (circled with white dashed lines) enter the spinal cord at ectopic loci instead of dorsal spinal cord.(B) PBS-loaded bead in E13 spinal cord. All labeled axons extend along the dorsal spinal cord, where they terminate.(C) High-power image of the bead in (A). Notice labeled axons surrounding the bead.(D) High-power image of an NT-3-loaded bead. Notice axons bundled around the bead.(E) High-power image of an NT-3-loaded bead. Notice the axons approaching the bead via the dorsal spinal cord.(F) High-power image of a PBS-loaded bead. No labeled fibers were observed around control beads.(G) Summary of our observations from E13 spinal cord DRG organotypic cultures. In control cultures fibers extend along the dorsal spinal cord, where they normally enter the gray matter at E13. In the presence of an ectopic NT-3 source localized at the midline, these axons grow towards the NT-3 bead. NT-3 also initiates axon growth from the DRGs, entering the spinal cord at ectopic lateral loci, growing towards the bead, surrounding the bead, forming nerve bundles, and branching around it.Scale bar, 175 μm (A and B), 100 μm (C–F). |
PMC529315_pbio-0020403-g006_757.jpg | What is shown in this image? | Chemoattraction Towards NT-3 Beads Placed in E13 Spinal Cord DRG Explant Co-Cultures(A) NT-3 bead placed in the midline of E13 WT spinal cord. Notice axons labeled through the DRGs (circled with black dashed lines) growing towards the bead (circled with white dashed lines) enter the spinal cord at ectopic loci instead of dorsal spinal cord.(B) PBS-loaded bead in E13 spinal cord. All labeled axons extend along the dorsal spinal cord, where they terminate.(C) High-power image of the bead in (A). Notice labeled axons surrounding the bead.(D) High-power image of an NT-3-loaded bead. Notice axons bundled around the bead.(E) High-power image of an NT-3-loaded bead. Notice the axons approaching the bead via the dorsal spinal cord.(F) High-power image of a PBS-loaded bead. No labeled fibers were observed around control beads.(G) Summary of our observations from E13 spinal cord DRG organotypic cultures. In control cultures fibers extend along the dorsal spinal cord, where they normally enter the gray matter at E13. In the presence of an ectopic NT-3 source localized at the midline, these axons grow towards the NT-3 bead. NT-3 also initiates axon growth from the DRGs, entering the spinal cord at ectopic lateral loci, growing towards the bead, surrounding the bead, forming nerve bundles, and branching around it.Scale bar, 175 μm (A and B), 100 μm (C–F). |
PMC529315_pbio-0020403-g006_750.jpg | What is the dominant medical problem in this image? | Chemoattraction Towards NT-3 Beads Placed in E13 Spinal Cord DRG Explant Co-Cultures(A) NT-3 bead placed in the midline of E13 WT spinal cord. Notice axons labeled through the DRGs (circled with black dashed lines) growing towards the bead (circled with white dashed lines) enter the spinal cord at ectopic loci instead of dorsal spinal cord.(B) PBS-loaded bead in E13 spinal cord. All labeled axons extend along the dorsal spinal cord, where they terminate.(C) High-power image of the bead in (A). Notice labeled axons surrounding the bead.(D) High-power image of an NT-3-loaded bead. Notice axons bundled around the bead.(E) High-power image of an NT-3-loaded bead. Notice the axons approaching the bead via the dorsal spinal cord.(F) High-power image of a PBS-loaded bead. No labeled fibers were observed around control beads.(G) Summary of our observations from E13 spinal cord DRG organotypic cultures. In control cultures fibers extend along the dorsal spinal cord, where they normally enter the gray matter at E13. In the presence of an ectopic NT-3 source localized at the midline, these axons grow towards the NT-3 bead. NT-3 also initiates axon growth from the DRGs, entering the spinal cord at ectopic lateral loci, growing towards the bead, surrounding the bead, forming nerve bundles, and branching around it.Scale bar, 175 μm (A and B), 100 μm (C–F). |
PMC529315_pbio-0020403-g006_752.jpg | What key item or scene is captured in this photo? | Chemoattraction Towards NT-3 Beads Placed in E13 Spinal Cord DRG Explant Co-Cultures(A) NT-3 bead placed in the midline of E13 WT spinal cord. Notice axons labeled through the DRGs (circled with black dashed lines) growing towards the bead (circled with white dashed lines) enter the spinal cord at ectopic loci instead of dorsal spinal cord.(B) PBS-loaded bead in E13 spinal cord. All labeled axons extend along the dorsal spinal cord, where they terminate.(C) High-power image of the bead in (A). Notice labeled axons surrounding the bead.(D) High-power image of an NT-3-loaded bead. Notice axons bundled around the bead.(E) High-power image of an NT-3-loaded bead. Notice the axons approaching the bead via the dorsal spinal cord.(F) High-power image of a PBS-loaded bead. No labeled fibers were observed around control beads.(G) Summary of our observations from E13 spinal cord DRG organotypic cultures. In control cultures fibers extend along the dorsal spinal cord, where they normally enter the gray matter at E13. In the presence of an ectopic NT-3 source localized at the midline, these axons grow towards the NT-3 bead. NT-3 also initiates axon growth from the DRGs, entering the spinal cord at ectopic lateral loci, growing towards the bead, surrounding the bead, forming nerve bundles, and branching around it.Scale bar, 175 μm (A and B), 100 μm (C–F). |
PMC529464_F1_758.jpg | What is the dominant medical problem in this image? | CT scan of the abdomen showing the involvement of abdominal wall muscles and adhesions to neighboring intestines. |
PMC529466_F2_759.jpg | What stands out most in this visual? | X-ray showing the intravascular catheter being fluoroscopically removed by means of a special endo-vessel grasper. |
PMC533873_F3_760.jpg | What object or scene is depicted here? | Major tissues of expression for C36B1.12. (A) Fluorescence micrograph of an L4 larvae hermaphrodite carrying a transcriptional fusion between gfp and a putative promoter of C36B1.12 expressed in neurons in the head. (C) Fluorescence micrograph of a young adult hermaphrodite carrying the same construct expressed in intestine. The observed intestinal expression is mostly located to posterior intestinal nuclei and is more prominent in younger worms. (B and D) Corresponding DIC images. Scale bars, 20 μm. |
PMC533873_F3_762.jpg | What is the central feature of this picture? | Major tissues of expression for C36B1.12. (A) Fluorescence micrograph of an L4 larvae hermaphrodite carrying a transcriptional fusion between gfp and a putative promoter of C36B1.12 expressed in neurons in the head. (C) Fluorescence micrograph of a young adult hermaphrodite carrying the same construct expressed in intestine. The observed intestinal expression is mostly located to posterior intestinal nuclei and is more prominent in younger worms. (B and D) Corresponding DIC images. Scale bars, 20 μm. |
PMC533873_F3_763.jpg | What does this image primarily show? | Major tissues of expression for C36B1.12. (A) Fluorescence micrograph of an L4 larvae hermaphrodite carrying a transcriptional fusion between gfp and a putative promoter of C36B1.12 expressed in neurons in the head. (C) Fluorescence micrograph of a young adult hermaphrodite carrying the same construct expressed in intestine. The observed intestinal expression is mostly located to posterior intestinal nuclei and is more prominent in younger worms. (B and D) Corresponding DIC images. Scale bars, 20 μm. |
PMC533873_F3_761.jpg | What is being portrayed in this visual content? | Major tissues of expression for C36B1.12. (A) Fluorescence micrograph of an L4 larvae hermaphrodite carrying a transcriptional fusion between gfp and a putative promoter of C36B1.12 expressed in neurons in the head. (C) Fluorescence micrograph of a young adult hermaphrodite carrying the same construct expressed in intestine. The observed intestinal expression is mostly located to posterior intestinal nuclei and is more prominent in younger worms. (B and D) Corresponding DIC images. Scale bars, 20 μm. |
PMC533873_F4_767.jpg | What's the most prominent thing you notice in this picture? | Major tissues of expression for F40F9.1 and F40F9.2. Fluorescence micrographs of an adult hermaphrodite carrying a transcriptional fusion between gfp and a putative promoter of F40F9.1 expressed in (A) neurons and pharyngeal muscle, (C) commissures (c) and the ventral nerve cord (vnc), and (E) body wall muscle (bwm) and hypodermis (h). (G) Fluorescence micrograph of an L4 hermaphrodite carrying a transcriptional fusion between gfp and a putative promoter of F40F9.2 expressed in the excretory system (exc), neurons, and pharyngeal muscle. (B, D, F, and H) Corresponding DIC images. Scale bars, 20 μm. |
PMC533873_F4_771.jpg | What's the most prominent thing you notice in this picture? | Major tissues of expression for F40F9.1 and F40F9.2. Fluorescence micrographs of an adult hermaphrodite carrying a transcriptional fusion between gfp and a putative promoter of F40F9.1 expressed in (A) neurons and pharyngeal muscle, (C) commissures (c) and the ventral nerve cord (vnc), and (E) body wall muscle (bwm) and hypodermis (h). (G) Fluorescence micrograph of an L4 hermaphrodite carrying a transcriptional fusion between gfp and a putative promoter of F40F9.2 expressed in the excretory system (exc), neurons, and pharyngeal muscle. (B, D, F, and H) Corresponding DIC images. Scale bars, 20 μm. |
PMC533873_F4_766.jpg | What is the core subject represented in this visual? | Major tissues of expression for F40F9.1 and F40F9.2. Fluorescence micrographs of an adult hermaphrodite carrying a transcriptional fusion between gfp and a putative promoter of F40F9.1 expressed in (A) neurons and pharyngeal muscle, (C) commissures (c) and the ventral nerve cord (vnc), and (E) body wall muscle (bwm) and hypodermis (h). (G) Fluorescence micrograph of an L4 hermaphrodite carrying a transcriptional fusion between gfp and a putative promoter of F40F9.2 expressed in the excretory system (exc), neurons, and pharyngeal muscle. (B, D, F, and H) Corresponding DIC images. Scale bars, 20 μm. |
PMC533873_F4_770.jpg | What object or scene is depicted here? | Major tissues of expression for F40F9.1 and F40F9.2. Fluorescence micrographs of an adult hermaphrodite carrying a transcriptional fusion between gfp and a putative promoter of F40F9.1 expressed in (A) neurons and pharyngeal muscle, (C) commissures (c) and the ventral nerve cord (vnc), and (E) body wall muscle (bwm) and hypodermis (h). (G) Fluorescence micrograph of an L4 hermaphrodite carrying a transcriptional fusion between gfp and a putative promoter of F40F9.2 expressed in the excretory system (exc), neurons, and pharyngeal muscle. (B, D, F, and H) Corresponding DIC images. Scale bars, 20 μm. |
PMC534093_F1_773.jpg | What is being portrayed in this visual content? | Grading of the transesophageally detected aortic lesions in the descending aorta. Grade 1: intimal thickening (left upper panel); Grade 2: small plaque indicated by arrow right upper panel; Grade 3: lower panels. On the lower panels: left a huge, multiple plaque, right: an ulcerated plaque with mobile part. |
PMC534093_F1_772.jpg | Can you identify the primary element in this image? | Grading of the transesophageally detected aortic lesions in the descending aorta. Grade 1: intimal thickening (left upper panel); Grade 2: small plaque indicated by arrow right upper panel; Grade 3: lower panels. On the lower panels: left a huge, multiple plaque, right: an ulcerated plaque with mobile part. |
PMC534093_F1_774.jpg | Can you identify the primary element in this image? | Grading of the transesophageally detected aortic lesions in the descending aorta. Grade 1: intimal thickening (left upper panel); Grade 2: small plaque indicated by arrow right upper panel; Grade 3: lower panels. On the lower panels: left a huge, multiple plaque, right: an ulcerated plaque with mobile part. |
PMC534093_F1_775.jpg | What is the central feature of this picture? | Grading of the transesophageally detected aortic lesions in the descending aorta. Grade 1: intimal thickening (left upper panel); Grade 2: small plaque indicated by arrow right upper panel; Grade 3: lower panels. On the lower panels: left a huge, multiple plaque, right: an ulcerated plaque with mobile part. |
PMC534613_F3_785.jpg | What object or scene is depicted here? | Immunostaining of NFκB protein in stroma (S), glandular epithelium (GE), squamous epithelium (SQ) and vessels (V) in cervical biopsies from the NP (left column, a, d, g, j), TP (middle column, b, e, h) and PP (right column, c, f, i, l) groups. Positive nuclear and cytoplasmic immunostaining is also observed in neuronal ganglions (G) (h). Some leukocytes, identified by their morphology, display positive NFκB staining (l, black arrowhead). A negative control where the primary antibody is replaced by rabbit IgG is shown in k. |
PMC534613_F3_777.jpg | Can you identify the primary element in this image? | Immunostaining of NFκB protein in stroma (S), glandular epithelium (GE), squamous epithelium (SQ) and vessels (V) in cervical biopsies from the NP (left column, a, d, g, j), TP (middle column, b, e, h) and PP (right column, c, f, i, l) groups. Positive nuclear and cytoplasmic immunostaining is also observed in neuronal ganglions (G) (h). Some leukocytes, identified by their morphology, display positive NFκB staining (l, black arrowhead). A negative control where the primary antibody is replaced by rabbit IgG is shown in k. |
PMC534613_F3_783.jpg | What is the core subject represented in this visual? | Immunostaining of NFκB protein in stroma (S), glandular epithelium (GE), squamous epithelium (SQ) and vessels (V) in cervical biopsies from the NP (left column, a, d, g, j), TP (middle column, b, e, h) and PP (right column, c, f, i, l) groups. Positive nuclear and cytoplasmic immunostaining is also observed in neuronal ganglions (G) (h). Some leukocytes, identified by their morphology, display positive NFκB staining (l, black arrowhead). A negative control where the primary antibody is replaced by rabbit IgG is shown in k. |
PMC534613_F3_780.jpg | What is being portrayed in this visual content? | Immunostaining of NFκB protein in stroma (S), glandular epithelium (GE), squamous epithelium (SQ) and vessels (V) in cervical biopsies from the NP (left column, a, d, g, j), TP (middle column, b, e, h) and PP (right column, c, f, i, l) groups. Positive nuclear and cytoplasmic immunostaining is also observed in neuronal ganglions (G) (h). Some leukocytes, identified by their morphology, display positive NFκB staining (l, black arrowhead). A negative control where the primary antibody is replaced by rabbit IgG is shown in k. |
PMC534613_F3_778.jpg | What is the dominant medical problem in this image? | Immunostaining of NFκB protein in stroma (S), glandular epithelium (GE), squamous epithelium (SQ) and vessels (V) in cervical biopsies from the NP (left column, a, d, g, j), TP (middle column, b, e, h) and PP (right column, c, f, i, l) groups. Positive nuclear and cytoplasmic immunostaining is also observed in neuronal ganglions (G) (h). Some leukocytes, identified by their morphology, display positive NFκB staining (l, black arrowhead). A negative control where the primary antibody is replaced by rabbit IgG is shown in k. |
PMC534613_F3_781.jpg | What is the main focus of this visual representation? | Immunostaining of NFκB protein in stroma (S), glandular epithelium (GE), squamous epithelium (SQ) and vessels (V) in cervical biopsies from the NP (left column, a, d, g, j), TP (middle column, b, e, h) and PP (right column, c, f, i, l) groups. Positive nuclear and cytoplasmic immunostaining is also observed in neuronal ganglions (G) (h). Some leukocytes, identified by their morphology, display positive NFκB staining (l, black arrowhead). A negative control where the primary antibody is replaced by rabbit IgG is shown in k. |
PMC534613_F3_779.jpg | What's the most prominent thing you notice in this picture? | Immunostaining of NFκB protein in stroma (S), glandular epithelium (GE), squamous epithelium (SQ) and vessels (V) in cervical biopsies from the NP (left column, a, d, g, j), TP (middle column, b, e, h) and PP (right column, c, f, i, l) groups. Positive nuclear and cytoplasmic immunostaining is also observed in neuronal ganglions (G) (h). Some leukocytes, identified by their morphology, display positive NFκB staining (l, black arrowhead). A negative control where the primary antibody is replaced by rabbit IgG is shown in k. |
PMC534613_F3_782.jpg | What is the main focus of this visual representation? | Immunostaining of NFκB protein in stroma (S), glandular epithelium (GE), squamous epithelium (SQ) and vessels (V) in cervical biopsies from the NP (left column, a, d, g, j), TP (middle column, b, e, h) and PP (right column, c, f, i, l) groups. Positive nuclear and cytoplasmic immunostaining is also observed in neuronal ganglions (G) (h). Some leukocytes, identified by their morphology, display positive NFκB staining (l, black arrowhead). A negative control where the primary antibody is replaced by rabbit IgG is shown in k. |
PMC534784_F3_787.jpg | What is being portrayed in this visual content? | Higher magnification of sections from the same experiment as in Figure 2 Both sections are 15 min after injection of active bovine LPL. The rat in panel A did not receive heparin; the rat in panel B had been given heparin five min before LPL. Green colour represents staining of LPL by the rabbit polyclonal antibody. Red colour represents staining of Kupffer cells by the ED2 antibody. The magnification was × 60 + zooming. |
PMC534784_F3_786.jpg | What is being portrayed in this visual content? | Higher magnification of sections from the same experiment as in Figure 2 Both sections are 15 min after injection of active bovine LPL. The rat in panel A did not receive heparin; the rat in panel B had been given heparin five min before LPL. Green colour represents staining of LPL by the rabbit polyclonal antibody. Red colour represents staining of Kupffer cells by the ED2 antibody. The magnification was × 60 + zooming. |
PMC534784_F4_788.jpg | What stands out most in this visual? | Ultrastructural localization of injected bovine LPL 125I-labeled active (upper panel) or inactive (lower panel) LPL was injected. Ten min later the rats were killed and sections of their livers were processed for autoradiography as detailed in the methods section. H, hepatocytes, EC, endothelial cell, KC, Kupffer cell. Bar: 2 μm. |
PMC534784_F4_789.jpg | What object or scene is depicted here? | Ultrastructural localization of injected bovine LPL 125I-labeled active (upper panel) or inactive (lower panel) LPL was injected. Ten min later the rats were killed and sections of their livers were processed for autoradiography as detailed in the methods section. H, hepatocytes, EC, endothelial cell, KC, Kupffer cell. Bar: 2 μm. |
PMC534784_F5_793.jpg | What is shown in this image? | Distribution of injected, inactive bovine LPL in livers, and the effect of heparin All sections are 15 min after injection of inactive bovine LPL. The sections in panels A and C are from rats that did not receive heparin; the sections in panel B and D are from rats that had been given heparin five min before the lipase. Green colour represents staining of LPL by the rabbit polyclonal antibody. Red colour represents staining of Kupffer cells by the ED2 antibody. Panels C and D show both stainings. The magnification was × 20 in panels A and B; × 60 + zooming in panels C and D. |
PMC534784_F5_790.jpg | Can you identify the primary element in this image? | Distribution of injected, inactive bovine LPL in livers, and the effect of heparin All sections are 15 min after injection of inactive bovine LPL. The sections in panels A and C are from rats that did not receive heparin; the sections in panel B and D are from rats that had been given heparin five min before the lipase. Green colour represents staining of LPL by the rabbit polyclonal antibody. Red colour represents staining of Kupffer cells by the ED2 antibody. Panels C and D show both stainings. The magnification was × 20 in panels A and B; × 60 + zooming in panels C and D. |
PMC534784_F5_792.jpg | What object or scene is depicted here? | Distribution of injected, inactive bovine LPL in livers, and the effect of heparin All sections are 15 min after injection of inactive bovine LPL. The sections in panels A and C are from rats that did not receive heparin; the sections in panel B and D are from rats that had been given heparin five min before the lipase. Green colour represents staining of LPL by the rabbit polyclonal antibody. Red colour represents staining of Kupffer cells by the ED2 antibody. Panels C and D show both stainings. The magnification was × 20 in panels A and B; × 60 + zooming in panels C and D. |
PMC534809_pbio-0020424-g005_794.jpg | What is shown in this image? | AFM Images of DAE-E Crystals(A) Several frequent morphologies that appear in most samples, including all-'0' (upper arrow) and ‘011011'-striped crystals (lower arrow). The all-'0' crystal may be a tube that opened upon adsorption to the mica.(B) A templated crystal. The identification of tiles in this crystal is given in Figure 1E. Crosses indicate mismatch errors. Asterisks indicate ‘1's on the nucleating strand.(C) A crystal containing 10 rows of error-free Sierpinski triangle. A red triangle marks a lattice defect in the input row.(D) Another Sierpinski triangle, better resolved.(E) A crystal containing a perfect 19 × 6 subregion. Individual tiles can be clearly seen; three tiles are outlined in the lower left. Unfortunately, this crystal landed atop a thin sliver of DNA (lower arrow), obscuring the central columns of the Sierpinski triangle. The upper arrow indicates a 4-tile wide tube, near the point where it opens. A pentagon marks a lattice dislocation. Scale bars are 100 nm. |
PMC534809_pbio-0020424-g005_795.jpg | What is the central feature of this picture? | AFM Images of DAE-E Crystals(A) Several frequent morphologies that appear in most samples, including all-'0' (upper arrow) and ‘011011'-striped crystals (lower arrow). The all-'0' crystal may be a tube that opened upon adsorption to the mica.(B) A templated crystal. The identification of tiles in this crystal is given in Figure 1E. Crosses indicate mismatch errors. Asterisks indicate ‘1's on the nucleating strand.(C) A crystal containing 10 rows of error-free Sierpinski triangle. A red triangle marks a lattice defect in the input row.(D) Another Sierpinski triangle, better resolved.(E) A crystal containing a perfect 19 × 6 subregion. Individual tiles can be clearly seen; three tiles are outlined in the lower left. Unfortunately, this crystal landed atop a thin sliver of DNA (lower arrow), obscuring the central columns of the Sierpinski triangle. The upper arrow indicates a 4-tile wide tube, near the point where it opens. A pentagon marks a lattice dislocation. Scale bars are 100 nm. |
PMC534809_pbio-0020424-g005_796.jpg | What is the principal component of this image? | AFM Images of DAE-E Crystals(A) Several frequent morphologies that appear in most samples, including all-'0' (upper arrow) and ‘011011'-striped crystals (lower arrow). The all-'0' crystal may be a tube that opened upon adsorption to the mica.(B) A templated crystal. The identification of tiles in this crystal is given in Figure 1E. Crosses indicate mismatch errors. Asterisks indicate ‘1's on the nucleating strand.(C) A crystal containing 10 rows of error-free Sierpinski triangle. A red triangle marks a lattice defect in the input row.(D) Another Sierpinski triangle, better resolved.(E) A crystal containing a perfect 19 × 6 subregion. Individual tiles can be clearly seen; three tiles are outlined in the lower left. Unfortunately, this crystal landed atop a thin sliver of DNA (lower arrow), obscuring the central columns of the Sierpinski triangle. The upper arrow indicates a 4-tile wide tube, near the point where it opens. A pentagon marks a lattice dislocation. Scale bars are 100 nm. |
PMC534809_pbio-0020424-g006_802.jpg | What is the central feature of this picture? | AFM Images of DAO-E Crystals(A) A large templated crystal in a 5-tile reaction (no R-11). A single ‘1' in the input row (asterisk) initiates a Sierpinski triangle, which subsequently devolves due to errors. Mismatch errors within ‘0' domains initiate isolated Sierpinski patterns terminated by additional errors at their corners.(B) A large untemplated fragment in a 5-tile reaction (no S-11). Large triangles of ‘0's can be seen. Crystals similar to this are also seen in samples lacking the nucleating structure.(C) Several large crystals in a 6-tile reaction, some with more zeros than ones, some with more ones than zeros. It is difficult to determine whether these crystals are templated or not.(D) An average of several scans of the boxed region from (C), containing roughly 1,000 tiles and 45 errors.(E) An average of several scans of a Sierpinski triangle that initiated by a single error in a sea of zeros and terminated by three further errors (a 1% error rate for the 400 tiles here). Red crosses in (D) and (E) indicate tiles that have been identified (by eye) to be incorrect with respect to the two tiles from which they receive their input. Scale bars are 100 nm. |
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