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we studied at the PSA morphological and behavioral C2 the effect of chronic ethanol consumption associated or not with a mild B1 deficiency episode we found that i B1 deficiency induced a significant decrease of the acetylcholinesterase ache activity both in cortex and hippocampus ii chronic ethanol treatment has no effect on cortical ache activity but induced a significant decrease of hippocampal enzyme activity iii the reduction in cortical and HPC ache activity induced by chronic ethanol treatment associated with a week thiamine deficiency was also significant and was greater than that induced by ethanol CT furthermore either chronic ethanol or TD induced a significant decrease in the release of acetylcholine ach in the stimulated condition using high potassium concentration and when both treatments were associated the decrease was even greater in the unstimulated condition the reduction in the release of ach was greater for ethanol treatment than for B1 deficiency openfield tests showed that only in the sniffing category were there significant differences among the experimental CG no morphological change was detected by optical microscopy suggesting that the injury process was in its initial stages in which only PET and behavioral changes are displayed in addition our PSA results indicate that cortical cholinergic susceptibilities to eyl increased from to years p or the results illustrate the sensitivity of hprt somatic mutation as a biomarker for populations with low dose radiation exposure and the dependence of this sensitivity on time elapsed since radiation exposure | MEDAL |
dendritic cells dc generated from adherent IP blood MNC cells pbmc by culturing with GM-CSF colonystimulating factor gmcsf and il were used to T0 in IVS of naive haptenspecific t cells and to analyse crossreactivities to related compounds dc were haptenderivatized with nickel sulphate ni or hydroxyethylmethacrylate hema followed by tumour necrosis factoralpha tnfalphainduced maturation before autologous TA and a cytokine cocktail of ilbeta il and il were added T3 t cell priming for days wells were split and challenged for another days with ni or hema and potentially CR haptens haptenspecificity of in vitro priming was demonstrated by proliferative responses to the haptens used for priming but not to the unrelated haptens highest priming efficiencies were obtained when both il and il were added to the cytokine supplement marked interferongamma Th1 release up to ngml was found when il was included in the cultures whereas il PR up to pgml was observed T3 addition of il alone or in combination with il nickelprimed TA showed frequent crossreactivities with other metals closely positioned in the periodic table ie palladium and copper whereas hemaprimed TA showed distinct crossreactivities with selected methacrylate congeners similar crossreactivities are known to occur in allergic patients thus in vitro t cell priming provides a promising tool for studying factors regulating cytokine synthesis and crossreactivity patterns of haptenspecific t cells | MEDAL |
dietary mannose is used to treat glycosylation deficient patients with mutations in PMI pmi but there is little information on mannose metabolism in model systems we chose the mouse as a vertebrate model intravenous injection of hmannose shows rapid equilibration with the extravascular pool and clearance t of min with of the label catabolized via glycolysis in h labeled glycoproteins appear in the plasma after min and increase over h various organs incorporate hmannose into glycoproteins with similar kinetics indicating direct transport and utilization liver and intestine incorporate most of the label and the majority of the liverderived proteins eventually appear in plasma hmannoselabeled CL and intestine organ cultures secrete the majority of their labeled proteins we also studied the longterm effects of mannose supplementation in the drinking water it did not cause bloating diarrhea abnormal behavior weight gain or loss or increase in hemoglobin glycation organ weights histology litter size and growth of pups were normal water NI of mice given mannose in their water was reduced to half compared to other groups mannose in blood increased up to fold from to microm and mannose in milk up to fold from to microm hmannose clearance organ CSD and uptake kinetics and hexose content of glycoproteins in organs were similar in mannosesupplemented and nonsupplemented mice mannose supplements had little effect on the TPS activity of phosphomannomutase manpmanp in different organs but specific activity of pmi in BB intestine muscle heart and lung gradually increased fold with increasing mannose NI thus LT mannose supplementation does not appear to have adverse effects on mannose metabolism and mice safely tolerate increased mannose with no apparent ill effects | MEDAL |
cyclooxygenase cox the key enzyme of prostanoid synthesis consists of the two isoforms cox and cox both recently noted to be constitutively expressed in rat lungs with a distinct profile of cellular CSD the responsiveness of pulmonary cox and cox expression to intravascular endotoxin lipopolysaccharide lps administration was investigated in isolated ventilated rat lungs bufferperfused with or without admixture of rat plasma immunohistochemical staining intensity was measured by a previously described method of silver enhancement and epipolarization image analysis both the cox mrna quantified in the whole lung TH and the cellular localization of cox were unchanged in response to lps in contrast time and dosedependent upregulation of cox mrna lung homogenate occurred and differential lps CR at the cellular level was observed upregulation of cox in cell types expressing this enzyme already under baseline conditions was noted in bronchial epithelial cells bronchial and V1 smooth muscle cells cells within the balt and myocytes of the C1 hilar veins de novo induction of cox occurred in endothelial cells and the majority of AM macrophages downregulation of cox was observed in perivascular and peribronchial macrophagelike cells moreover differential impact of plasma components was noted for the C1 majority of cells cd surface expression correlated with cox responsiveness to lps independent of plasma whereas the presence of plasma components was a prerequisite for the lps response in cdnegative cells lps did not provoke physiological changes in the perfused lungs but markedly enhanced baseline prostanoid generation we conclude that lpsinduced cox regulation occurs in a complex cellspecific manner which may be relevant for pathogenetic sequelae in septic lung injury and acute respiratory failure | MEDAL |
the exact CSD of nitric oxidesynthases nos and the notarget enzyme soluble guanylyl cyclase sgc in the cochlea and vestibular organ is an issue of current discussion the existence of nosisoforms in the cochlea of the guinea pig has been described recently while information about the vestibular system are still rare and nonsatisfying in order to gain more information IS was performed using specific antibodies to nos iiii and to sgc on paraffin sections of CR temporal bones from mice nos iii could be detected in cochlea and vestibular ganglion cells in nerve MF in outer hair cells of the cochlear and in the sensory epithelium of the maculae also the spiral AL and the limbus epithelium was positive to nos iii nos i was found in the sensory epithelium of the maculae and cristae ampullares outer and inner hair cells of the cochlea in nerve MF and in GCs in contrast to that nos ii could not be detected at all furthermore a strong nos i immunoreaction was displayed on the endosteum of the bone while the periosteum was lacking of nos nos detection was accompanied by immunoreactivity to sgc the findings imply that nos i and iiigenerated no is involved in neurotransmission and other regulative processes in the vestibulocochlear system | MEDAL |
we consider estimation of various probabilities after termination of a group sequential phase ii trial a motivating example is that the stopping rule of a phase ii oncologic trial is determined solely based on response to a drug treatment and at the end of the trial estimating the rate of toxicity and response is desirable the conventional maximum likelihood estimator sample proportion of a probability is shown to be biased and two alternative estimators are proposed to correct for bias a biasreduced estimator obtained by using whiteheads biasadjusted approach and an unbiased estimator from the raoblackwell method of conditioning all three estimation procedures are shown to have certain invariance property in bias moreover estimators of a probability and their bias and precision can be evaluated through the observed response rate and the stage at which the trial stops thus avoiding extensive computation | MEDAL |
the objective of this study was to assess somatic and inherited androgen receptor gene mutations in families with only one affected individual | MEDAL |
the objective of this umbrella review is to determine the characteristics of dietary interventions delivered any time before during or after pregnancy for women living in developed countries identified in quantitative systematic reviews as effective for improving mother and infant health outcomes and feasible for translation into practicespecifically the review will address the following questionsfor the purpose of this overview dietary interventions encompass the broad scope of dietary manipulations including the direct provision of food andor nutrients to participants nutrition education nutrition counseling nutrition care teams or any combination of these four MI typesboth a narrative and quantitative summary on the effect of maternal dietary interventions on maternal and infant health outcomes as compared with usual care will be provided the quantitative summary of the maternal and infant outcomes will be supported by an appraisal of the certainty of evidence of the effects and the strength of the recommendations using the grading of recommendations assessment development and DUE grade framework | MEDAL |
antiphospholipid syndrome aps is an autoimmune disease and its most critical pathologic process is thrombosis which may explain most of the clinical features acute management of thrombosis involves immediate anticoagulation acute PT VT can be managed with TT to reduce the longterm complications of the postthrombotic syndrome pain swelling skin discoloration or ulceration and perform recanalization of occluded vessels however thrombolytic therapies are associated with high risks of bleeding to our knowledge this is the first report of EDH mimicking transverse myelitis T3 catheterdirected thrombolysis in a patient with primary aps a yearold male was admitted with sudden ON pain and swelling on left LE venography demonstrated multiple thrombi on SFV CFV common iliac vein and external iliac vein laboratory tests indicated the presence of igm aCL he was diagnosed with primary aps with multiple venous thrombi he was treated with urokinase h as TT T3 day he complained both leg weakness and urinary dysfunction t and tweighted magnetic resonance images of spine showed about cmsized mass suggesting hematoma on the posterior epidural space at TL area despite the successful evacuation of hematoma neurologic symptoms persisted and he is now receiving aspirin warfarin and PT | MEDAL |
anesthetics and sedatives contribute to postoperative immunosuppression interleukin il is a chemotactic and activating factor that mediates neutrophil adhesion and margination and is essential for host defense we investigated the effect of anesthetics on isolated human polymorphonuclear leukocyte production of il healthy human polymorphonuclear WBCs were isolated using a singlestep density gradient and stimulated with lipopolysaccharide in the presence of varying concentrations of propofol or midazolam for up to h il was measured in both culture supernatants and cell lysates using enzyme immunoassay and il mrna in cells was measured using RNA blotting and phosphorimaging data were analyzed using kruskalwallis analysis of variance or the mannwhitney utest as AGA lipopolysaccharide increased extracellular accumulation of interleukin which was suppressed by both propofol p and midazolam p however intracellular il increased with SE to lipopolysaccharide p and remained increased with both anesthetics RNA blot analysis also revealed increased il mrna levels in the presence of both midazolam and propofol which was confirmed by molecular imaging these data strongly suggest that the anesthetics modulate transport or secretion of il protein from the cell suppression of il by anesthetics and sedatives may predispose postoperative and intensive care patients to infection | MEDAL |
maturation of BB structure has a different rhythm in girls and boys from the viewpoint of cognitive processes the first decade of life is the most important developmental stage after intrauterine life cognitive functions will essentially develop also during the second decade gender differences have been noted already in early adolescence behavioral disturbances and substance abuse problems as well as attentiondeficit disorders are more common in boys whereas depression anxiety and EDs are more common in girls regarding psychiatric morbidity onset of puberty and late adolescence are periods of special vulnerability | MEDAL |
acetylcholinesterase ache the enzyme that degrades acetylcholine is a heterogeneous enzyme that can be separated into multiple molecular forms a tetrameric membranebound form g and a monomeric soluble form g are the two predominant enzyme species in rat BB the distribution of ache molecular forms was defined by SU density gradients of anatomical regions of postmortem brains from patients with PSD of the alzheimer type dat and ND controls of similar ages the results demonstrate an overall loss of protein and enzyme activity in all Az of the dat brains studied and a selective loss of the g form of ache in brodmann areas and and the amygdala there was no NC in the gg ratio in areas and in the hippocampus or in the cerebellum there was a high regional rho of the gg ratios with published values for choline acetyltransferase activity but lower correlation with total ache activity we propose that there is a predominant loss of the g form of ache in dat and that this loss is correlated with the degeneration of presynaptic elements | MEDAL |
successive tactile stimuli delivered one to each hand are referred to spatial representation before they are ordered in time yamamoto and kitazawa in nat neurosci a in the present T0 we examined if this applies even when they are delivered unilaterally to fingers of a single hand tactile stimuli were delivered lefttorightward relative to the body ndrdth or in reverse with SOA of ms simultaneously with the delivery of tactile stimuli three of nine small squares arranged in a matrix of x were turned on as if they appeared near the tips of the fingers although subjects were instructed to ignore the visual stimuli and make a forced choice between the two orders of tactile stimuli the correctjudgment probability depended on the direction of visual stimuli it was greater than when the direction of visual stimuli matched that of the tactile stimuli but less than when they were opposite to each other when the right hand was rotated counterclockwise on the horizontal plane degrees so that the fingers were pointing to the left the preferred direction of visual stimuli that yielded the peak correct judgment was also rotated although not to the full extent these results show that subjects cannot be basing their tactile temporal order judgment solely on a somatotopic map but rather on a spatial map on which both visual and tactile signals converge | MEDAL |
chemical physical and ecological systems passing through a saddlenode bifurcation will momentarily find themselves balanced at a semistable steady state if perturbed by noise such systems will escape from the zerosteady state with escape time CS to noise when the MM is D2 to include space this leads to different points in space escaping from zero at different times and uniform initial conditions nucleate into sharp peaks spreading randomly across a nearly uniform background a phenomenon closely resembling nucleation during Tm we use C1 deviation theory to determine burst shape and temporal scaling with respect to noise amplitude these results give a prototype for a particular form of patternless symmetry breaking in the vicinity of a stability boundary and demonstrate how microscopic noise can lead to macroscopic effects in such a region | MEDAL |
optimal perioperative antiplatelet therapy in patients with coronary stents undergoing surgery still remains poorly defined and a matter of debate among cardiologists surgeons and anaesthesiologists surgery represents one of the most common reasons for premature antiplatelet therapy discontinuation which is associated with a significant increase in mortality and major adverse CE in particular stent thrombosis CPGs provide little support with regardthesis of oil compounds in perilla will help in understanding RII systems of AA metabolites and in elucidating reaction mechanisms for NK product synthesis in this study two types of alcohol dehydrogenases an aldoketo reductase akr and a geraniol dehydrogenase gedh which are thought to participate in the biosynthesis of perilla essential oil components such as citral and perillaldehyde were isolated from three pure lines of perilla these enzymes shared high amino acid sequence identity within the genus perilla and were expressed regardless of oil type the overall reaction from geranyl diphosphate to citral was performed in vitro using geraniol synthase and gedh to form a C1 proportion of citral and relatively little geraniol as MRP the biosynthetic pathway from geranyl diphosphate to citral the main compound of citraltype perilla essential oil was established in this study | MEDAL |
a triwavelength synchronous modelocking operation of a diodepumped ybycaf laser has been investigated by using a semiconductor saturable absorber mirror at μm the triwavelength synchronous modelocking pulses were centered at and nm an IP was obtained in the autocorrelation trace where the beat pulse width was ps and the repetition rate was thz the maximum average SO SP was mw with PP width ps and TR rate of mhz | MEDAL |
a significant rho was found between heightened PV and increased red blood cell aggregation and the severity of coronary artery disease at low shear rates and exhausted vasomotion these rheological factors can cause a reversible loss of fluidity the reduced fluidity may induce a limitation in microcirculatory flow due to the viscus resistance rheological treatments aim at restoration of impaired perfusion by decreasing plasma viscosity and thus diminishing red blood cell aggregation further therapeutic measures tend to improve red blood cell deformability because of limited coronary reserve in coronary i.a. disease hemodilution therapy is contraindicated except the cases with polyglobulia thrombolytic therapy in AIM causes a significant reduction of plasma viscosity and red cell aggregation for at least hours this improvement in blood fluidity may beneficially influence the reperfusion of ICM Az a therapy with orally AS hemorheological drugs pentoxifylline and buflomedil can be discussed as an additive treatment in severe AP refractory to TPS medical therapy since these drugs increase fluidity and inhibit platelet aggregation the defibrination may cause thrombotic and bleeding complications in the EP of treatment coronary small vessel disease represents a rare type of coronary heart disease this disease is defined by normal Epi coronaries and reduced PD reserve in disorders of coronary microcirculation with abnormal rheology waldenstroms macroglobulinemia rheological treatment is a rational and causal therapy | MEDAL |
a comparison was made of the oxidative AS os levels of patients with either viral or bacterial severe communityacquired pneumonia scap and of patients without infection healthy volunteers hv and patients with AIM ami | MEDAL |
parental CA is a significant problem for adolescent and young adult F1 to understand the extent of the problem of parental CA for australian F1 data regarding those impacted are required the aim of this T0 was to enumerate and describe the characteristics of western australian adolescent and young AD offspring years and their parents with CA using linked population data | MEDAL |
splenic flexure volvulus is the least common form of colonic volvulus preoperative radiological diagnosis is usually made presumptively based on plain radiographs andor barium contrast enema study in patients presenting with acute LB one we report a case in which multidetector computer tomography ct findings were diagnostic of this condition thus highlighting the usefulness of multiplanar reformatting | MEDAL |
the effects of organic loading rate on the performance and stability of anaerobic codigestion of municipal biomass waste mbw and waste activated sludge was were investigated on a pilotscale reactor the results showed that stable operation was achieved with organic loading rates olr of kg volatile solid vs md with vs reduction rates of and volumetric biogas production of m md a maximum methane production rate of m md was achieved at olr of kg vs md and hydraulic PR time of days with increasing olrs the anaerobic reactor showed a decrease in vs removal rate average ph value and methane concentration and a increase of volatile HFA concentration by monitoring the biogas production rate bpr the anaerobic digestion system has a higher acidification risk under an olr of kg vs md this result remarks the possibility of relating bioreactor performance with bpr in order to better understand and monitor anaerobic digestion process | MEDAL |
kinetochores are multiprotein complexes that power chromosome movements by tracking microtubules plusends in the MI spindle human kinetochores bind up to microtubules even though single microtubules can generate sufficient force to move chromosomes here we show that high microtubule occupancy at kinetochores ensures robust chromosome segregation by providing a strong mechanical force that favours segregation of merotelic attachments during anaphase using low doses of the microtubulestargeting agent bal we reduce microtubule occupancy and observe that spindle morphology is unaffected and bioriented kinetochores can still oscillate with normal intrakinetochore distances interkinetochore stretching is however dramatically reduced the reduction in microtubule occupancy and interkinetochore stretching does not delay satisfaction of the spindle assembly checkpoint or induce microtubule detachment via aurorab kinase which was so far thought to release MTs from kinetochores under low stretching rather partial microtubule occupancy slows down anaphase a and increases incidences of lagging chromosomes due to merotelically attached kinetochores | MEDAL |
Pc group pcg proteins function as high molecular weight complexes that maintain transcriptional repression paation factor alpha eifalpha pkr is also a AS kinase and its levels increase during ageing here we show that pkr activation and eifalpha phosphorylation play a significant role in apoptosis of neuroblastoma cells and primary neuronal cultures induced by the betaamyloid abeta peptides the A23187 and flavonoids the phosphorylation of eifalpha and the number of apoptotic cells were enhanced in overexpressed wildtype pkr Neuro-2a exposed to abeta peptide while dominantnegative pkr reduced eifalpha phosphorylation and apoptosis induced by abeta peptide primary cultured neurons from pkr knockout mice were also less CS to abeta peptide toxicity activation of pkr and eifalpha pathway by abeta peptide are triggered by an increase in intracellular calcium because the intracellular calcium chelator baptaam significantly reduced pkr phosphorylation taken together these results reveal that pkr and eifalpha phosphorylation could be involved in the molecular signalling events leading to neuronal apoptosis and death and could be a new target in neuroprotection | MEDAL |
the anhydroglucose chains of cellulose possess hydroxyls that facilitate different chemical modification strategies to expand on or provide new applications for membranes produced by the bacteria gluconacetobacter xylinus conjugation with biomolecules such as proteins especially by the amine CG is of great value and interest for the production of biomaterial derivatives from bacterial cellulose to assist in these modifications cellulose was succinylated in order to prevent steric hindrance and to create an att point for conjugation bacterial cellulose CM were first treated in dichloromethane and reacted with succinic anhydride through a series of conditions the membrane structure remained intact after these first processes and the product was confirmed by infrared spectroscopy and solid state nuclear magnetic resonance and characterized by xray diffraction thermogravimetry and atomic force microscopy hydrolyzed collagen was used as a model protein of interest to be conjugated to these CM which furnished a biomaterial functionalized over its surface | MEDAL |
the distribution of somatostatinimmunoreactive somir elements in the cerebellar cortex of the rat has been studied at different stages of PN development from birth to day and in AD animals using immunohistochemistry the results showed that in vermis of new born animals there are three main CG of somir structures within the SC which subsequently spread along the purkinje cell layer in addition both in the vermis and in the lateral lobes numerous more evenly distributed sompositive cells and SF could be seen somir golgi cells PCs and CFs could then be recognized during the subsequent developmental stages in the vermal zone somir PCs formed patches which seemed to be part of a sagittal columnar or bandlike organization this was most obvious between days and of PN development subsequently there was a reduction in the number of immunoreactive PCs but a patchy disposition remained in addition high numbers of somir purkinje and golgi cells and also climbing fibers were identified in the flocculus and paraflocculus at all stages of development studied and they were also seen in the AD rats in these regions in the lateral lobes expression of somlike immunoreactivity li decreased and almost completely disappeared in AD animals the present results demonstrate that a som or a somli peptide can be transiently detected in many purkinje and golgi cells in the cerebellar cortex suggesting a role in events related to developmental processes however in some regions and structures somli can be seen also in AD animals | MEDAL |
the pharmacokinetics of benflumetol as a fixed combination artemetherbenflumetol cgp following three regimens regimen a four tablets at and h mg AM mg benflumetol regimen b two tablets at and h mg artemether mg benflumetol regimen c four tablets at and h mg artemether mg benflumetol were investigated in patients with acute uncomplicated falciparum malaria all patients showed a rapid initial response with a median parasite clearance time of and h and a fever clearance time of and h for regimens a b and c respectively in nine patients two four and three patients in regimens a b and c respectively however parasitemia reappeared in the peripheral blood smear between days and the pharmacokinetics of benflumetol were highly VL with coefficients of R2 in pharmacokinetic parameters ranging from to absorption and beta of benflumetol were relatively slow median cmax per dose first dose was significantly higher in regimen b ngmlmg dose than in regimen a ngmlmg dose and regimen c ngmlmg dose mean tz in regimen c h was significantly shorter than in regimen a h and regimen b h in patients on regimens a and b who showed a sensitive response PC of benflumetol were oil chem soc in separate studies using xray diffraction the cubic phase larsson et al j sci food agric and the inverted hexagonal hii phase caffrey m biophys j were shown to be the dominant phases we have resorted to the traditional isoplethal diffraction method for constructing the equilibrium phase diagram of monoerucin in water with a view to resolving the controversy regarding the thermotropic and lyotropic properties of the system the study confirms the presence of the pure hii phase that extends from ca to ww water and from ca to degrees c and its coexistence with excess water above ca ww water in the same temperature range the LT region of the diagram is dominated by the lamellar crystal lc phase with the existence of the lamellar liquid crystal l alpha and cubiciad phases at intermediate temperatures and hydration levels differential scanning calorimetry and polarizing light microscopy were used to confirm the reported equilibrium phase SMB full structure characterization of the system and how phase microstructure depends on sample hydration and temperature are reported | MEDAL |
bryostatin bryo a macrocyclic lactone stimulates some but not all of the biologic effects which are induced by phorbol esters pes in vitro it competes with pes for IB to whole cells and activates the calciumphospholipiddependent protein kinase pkc to examine whether bryo like pes is able to stimulate the nonadherent growth of cells we used the mouse epidermal cell L1 jb which is stimulated by pes to grow in soft agar like pes bryo stimulates both the Ad and nonadherent growth of these cells but bryo microm is less active than equivalent concentrations of pes to attempt to explain the biologic differences between these two agents we examined the modulation of pkc by both pes and bryo in a phosphotransferase assay using partially purified pkc from jb cells bryo microm stimulated less phosphorylation of histone substrate than did pma aost disease in balbc x aj f mice with the injection of lymphoid cells from the parental aj CS these animals developed glomerulonephritis forefoot edema alopecia splenomegaly and lymphadenopathy to various degrees and all developed antinuclear antibodies to determine whether these antibodies were directed against the small nuclear ribonucleoprotein snrnp particles that are characteristic targets for autoimmune responses in human rheumatic diseases sera were studied in the p immunoprecipitation and immunoblotting assays among mice antibodies to snrnp developed in these antibodies usually reached maximal levels about wk after induction of GVHD and generally fell thereafter however two mice developed antibodies to snrnp between the th and th wk of followup sera from six mice strongly recognized the u snrnp and an additional serum strongly bound both the u and u particles several sera contained lower C2 of antibodies specific for the u and possibly preu snrnp particles in immunoblots sera that immunoprecipitated the u snrnp bound epitopes located on its da a bb andor c polypeptides sera that immunoprecipitated the u snrnp recognized a da polypeptide these polypeptides are known to bear the autoantigenic epitopes that are recognized by human sera containing antiu rnp and antiu rnp autoantibodies we conclude that chronic GVHD in mice provides a model for the study of the autoimmune responses that characterize human diseases such as mixed connective tissue disease scleroderma and sle | MEDAL |
in may a report of two workers from the same construction equipment manufacturing plant who were admitted to hospital with legionnaires disease confirmed by urine antigen prompted an outbreak investigation both cases were middle aged men smokers and with no travel leisure or other common community SE to legionella sources there were no wet cooling towers at the plant or in the surrounding area no increase in NMRD or worker absenteeism occurred at the plant during the preceding month wider case ascertainment including alerts to hospitals and medical practitioners yielded no further cases the environmental investigation and sampling of water systems for legionella identified a legionella pneumophila serogroup mab b count of xcful in water samples from an aqueous metal pretreatment tunnel which generates profuse water aerosol drainage cleaning and biocide treatment using thiazalone eliminated legionella from the system | MEDAL |
there is indication that RT growth is sustained by subpopulation of cells with stemlike features but little is known on their genomic characterization and their genetic stability we report a detailed molecular cytogenetic characterization using spectral karyotyping and FL in situ hybridization of parental serumcultured AC and their spheregrowing stemlike counterpart before and after differentiation from six cell lines established from ST our findings indicate increased cytogenetic complexity in spheregrowing stemlike and their differentiated adherent cells compared to parental adherent component suggesting the existence within cell lines of heterogeneous and genetically unstable subpopulations of cells endowed with stemlike features | MEDAL |
the thyroid tumours and BG goiterous and adenomatous lesions induced in rats with diisopropanolnitrosamine dipn plus MTU mtu and regenerative thyroid tissues after wounding were studied by lectin histochemistry ten weeks after cessation of the carcinogen treatments carcinomas invading the surrounding tissues and blood vessels and PTC micronodules were formed in the thyroid tissues in GA the carcinoma lesion was solitary and the papillary micronodules were multiple in a single thyroid gland among the lectins tested maclura pomifera mpa and solanum tuberosum sta showed specific IB with both carcinoma and PTC micronodule lesions but not with the BG goiterous and adenomatous lesions and regenerative thyroid tissues the former both lesions showed higher labelling indices with budr or hthymidine and poorer thyroglobulin accumulation than the latters thereby indicating their enhanced proliferative capability and depressed potency of cytodifferentiation the common cytological and histochemical properties of carcinoma lesions and papillary micronodules allow us to regard the latter as preinvading carcinoma lesions the lectins mpa and sta may be therefore used as the specific markers of malignancy in rat thyroid carcinogenesis | MEDAL |
total cholesterol hdl ldl and triglycerides were assayed in the blood SS and bile from the CL and gallbladder of both normal individuals and patients with cholelithiasis it was found that all assayed parameters are significantly higher in CL an increase in total cholesterol and hdl was seen in the bile from the liver whereas an increase in the level of unstable ldl by about four fold with simultaneous decrease in hdl level were found in the bile from the GB these differences in lipoprotein fractions level in patients with cholelithiasis indicate systemic disorders in cholesterol metabolism | MEDAL |
attempts were made to find prognostic AF in myeloma in deceased patients urinary CS chains skeletal lesions and the quantity of the monoclonal protein fraction in the SS were correlated to prognosis in contrast to the electrophoretic mobility of the monoclonal fraction the hemoglobin the serum creatinine value the serum calcium or the intestinal calcium absorption skeletal calcium uptake was only numerically higher in mild myeloma than in advanced myeloma since these findings partially agreed with the staging procedure previously proposed by salmon a modification of this procedure was used to stage myeloma patients survival was statistically significantly shorter in stage iii than in stage i a differentiated treatement with melphalanprednisone in stage i cytoxan infusions in stage ii and vincristinecytoxanprednisone in stage iii is proposed a preliminary comparison of nine patients in stage iiiii given intensive treatment with given MP suggests a numerically but not as yet a statistically significant increase in survival in the intensively treated group which seems to have an year survival | MEDAL |
repeat reduction mammaplasty is an uncommonly performed procedure currently no clear operative guidelines of management exist sixteen patients breasts with a mean age of years range to years underwent repeat breast reduction over an year period before the first reduction the mean notch to nipple distance was cm range to cm and mean nipple to inframammary crease distance was cm range to cm the mean mass of tissue excised was g per breast a number of different pedicles were used six inferior five superior four superomedial one unknown all patients subsequently developed pseudoptosis the nipple to inframammary crease distance was a mean of cm having initially been set at cm before the second procedure at the second T0 two patients three breasts had their initial pedicles transected and the NAC moved and both patients developed vascular compromise of the nippleareola complex two breasts where the same pedicle was used in the second T0 five patients breasts one patient developed unilateral nippleareola complex necrosis in eight patients because of the OD of pseudoptosis the nipple was in a satisfactory position and therefore only an VPI wedge of tissue required excision this was performed without nippleareola complex compromise irrespective of the initial pedicle the mean mass of tissue excised in the second operation was g per breast range to g fourteen patients were available for followup T3 a mean of years range months to years following the repeat reduction mammaplasty in the repeat breast reduction where nippleareola complex transposition is planned the initial pedicle should be reused to maintain NAC perfusion where the initial pedicle is not known a free nipple VG may be the safest option in patients with pseudoptosis in whom the nipple does not require transposition an inferior wedge of tissue can be safely excised irrespective of the initial pedicle | MEDAL |
this work describes gonadotropic gth cells and their morphological and immunohistochemical changes during the spermatogenic cycle of serrasalmus maculatus continuous spermatogenesis and pimelodus maculatus seasonal spermatogenesis gth cells widely distributed in the PT pars distalis of the adenohypophysis were characterized as roundshaped cells with eccentric nucleus and cytoplasm with basophilic secretory LG and a variable number of vacuoles for both species immunohistochemistry against βfolliclestimulating hormone fsh and βluteinizing hormone lh in adjacent sections showed two separated gthproducing cell populations and a third population where both gths are expressed in the same cell for both species in the seasonal spermatogenesis of p maculatus gth cells seemed to be more abundant during developing and spawning capable phases in contrast no cyclic changes were detected in the continuous spermatogenesis of s maculatus except for the strong immunoreaction for fsh and lh in males with intense spermiogenesis we conclude that changes reported here might reflect the type of spermatogenic cycle seasonal or continuous which are under different regulatory mechanisms environmental and internal cues controlling the reproduction in these species | MEDAL |
the objective of this T0 was to characterize empirically the association between vaccination coverage and the size and occurrence of measles epidemics in germany in order to achieve this we analysed data routinely collected by the robert koch institute which comprise the weekly number of reported measles cases at all ages as well as estimates of vaccination coverage at the average age of entry into the school system coverage levels within each federal state of germany are incorporated into a multivariate timeseries MM for infectious disease counts which captures occasional outbreaks by means of an AR component the observed incidence DP of measles for all ages is best described by using the log proportion of unvaccinated school starters in the autoregressive component of the MM | MEDAL |
numerous studies over the past decade support the claim that infants are equipped with powerful statistical language learning mechanisms the primary evidence for statistical language learning in word segmentation comes from studies using artificial languages continuous streams of synthesized syllables that are highly simplified relative to real speech to what extent can these conclusions be scaled up to natural language learning in the current experiments englishlearning monthold infants ability to track transitional probabilities in fluent infantdirected italian speech was tested n the results suggest that infants are CS to transitional probability cues in unfamiliar NK language stimuli and support the claim that statistical learning is sufficiently robust to support aspects of realworlting times of the slice artifacts at subsample precision this RPA yielded efficient imaging artifact reduction the amplitude at the dominant frequency was att by db the median values over eeg channels and the residual signal at its best was practically free from sharp transients even with hz sampling frequency and without further residual artifact reduction algorithms the presented methods may reduce the need for postprocessing of the residual signal T3 the template subtraction and may help to preserve the eeg bandwidth | MEDAL |
to compare tulobuterol patch and oral SS in terms of tau and safety in children with mild or mof bovine serum fetal calf serum fcs compared to fcs the prp addition to the SC medium increased the msc colony number and average size in primary cell cultures and in cell line cultures the prp promoted cell proliferation also in conditions where the fcs had not a proliferation stimulating effect due to either the nature of the cells and the tissue of origin such as human articular chondrocytes from elderly patients or to the critical low density cell seeding such as for hela cells in summary the standardized prp formulation would provide an offtheshelf product to be used for the selection and expansion of several cell types also in critical cell culture conditions | MEDAL |
the CSD of strains producing metallobetalactamase among strains of serratia marcescens was investigated all of these strains were isolated in seven general hospitals located in aichi prefecture japan from april to may southern hybridization analysis suggested that four s marcescens strains ak ak ak and ak had a metallobetalactamase genes similar to the blaimp gene found by our laboratory e osano y arakawa r wacharotayankun m ohta t horii h ito f yoshimura and n kato antimicrob agents chemother and these four strains showed resistance to carbapenems as well as to the other broadspectrum betalactams in particular strains ak ak and ak showed an extraordinarily highlevel resistance to imipenem mics or microgramsml whereas CS ak demonstrated moderate imipenem resistance mic microgramsml the imipenem resistance of ak was transferred to escherichia coli csh by conjugation with a frequency of the dna probe of the blaimp gene hybridized to a C1 plasmid approximately kb transferred into the e coli transconjugant as well as to the C1 plasmids harbored by ak on the other hand although we failed in the conjugational transfer of imipenem resistance from strains ak ak and ak to e coli csh imipenem resistance was transferred from these strains to e coli hb by transformation a plasmid approximately kb was observed in each transformant which acquired imipenem resistance the CAA sequence at the n terminus of the enzyme purified from strain ak was MZ to that of the MBL imp in contrast strains es ak and ak which were moderately resistant to imipenem mics or to or microgramsml had no detectable blaimp gene as a conclusion of clinically isolated s marcescens strains in aichi prefecture japan in were resistant to imipenem mics or microgramsml and strains which showed highlevel imipenem resistance because of acquisition of a plasmidmediated blaimplike MBL gene had already proliferated as nosocomial infections at least in a GA hospital | MEDAL |
twentyfour children with CP chickenpox were referred for ophthalmic assessment because of lesions on the lids and in the eye the appearance of these lesions their treatment and their complications are discussed twentyfive percent of these children were documented to have an anterior uveitis | MEDAL |
the scientists of protein structure and function and molecular immunology departments of the palladin institute of biochemistry nas of ukraine under the supervision of member of nasu and namsu prof s v komisarenko and corresponding member of nasu prof e v lugovskoy have made the real breakthrough in the field of research of the mechanisms of Fn polymerization and formation of Fn FR of thrombi the immunodiagnostic testsystems for the evaluation of the risk of thrombus formation were developed for the first time researches have obtained the monoclonal antibodies to fibrinogen Fn ddimer and their fragments these monoclonal antibodies were used as molecular probes for the localization of newly detected fibrin polymerization CS obtained antibodies with high affinity interact with fibrinogen ddimer and soluble fibrin â main markers of the risk of thrombus formation they were used for the development of the immunodiagnostic testsystems to quantify these markers in human blood plasma for the DUE of the state of haemostasis system detection of prethrombotic states disseminated intravascular coagulation PCD of thrombosis and monitoring of antithrombotic and fibrinolytic therapy the successful trial of developed testsystems was carried out in clinics of ukraine and the state registration was obtained for the implementation of them into the clinical practice presented works were awarded state prize of ukraine in science and technology | MEDAL |
the present T0 describes and compares PET properties of nuli cells and primary human nasal epithelial cells hnec including tlr expression and function differences in mRNA were identified for nontlr genes that play a role in tlr response pathways however experiments comparing tlr gene expression for both nuli cells and hnecs indicated conserved expression in both cell types stimulation of the two cell types resulted in a conserved response to tlr agonists but in differences in response to agonists for tlr and tlr hnecs were much more susceptible to infection with staphylococcus aureus than nuli cells furthermore when cultured at airliquid interface ali nuli cells possessed much lower transepithelial resistance than primary hnec and did not exhibit maintenance of cell morphology or mucous production which was observed in hnecs nor did they produce the characteristic interconnecting DP of tight junction complexes at the apicolateral margin of adjacent cells caution should therefore be exercised when selecting cell lines for immunological studies and a thorough screen of properties relevant to the T0 should always be carried out prior to commencement | MEDAL |
various reconstructive techniques have been applied to the management of difficult wounds in the dog wounds in the distal extremities and on the face are hard to manage by conventional methods a free cutaneous flap based on the superficial cervical vessels was developed and used in a series of experimental and clinical cases six of eight experimental orthotopic transfers survived the two failures were thought to be related to technical error the seven flaps used in clinical cases all survived and successfully resolved the dogs clinical problems | MEDAL |
pathogentargeted transcriptional profiling in human sputum may elucidate the physiologic state of mycobacterium tuberculosis m tuberculosis during infection and treatment however whether m tuberculosis transcription in sputum recapitulates transcription in the lung is uncertain we therefore compared m tuberculosis transcription in human sputum and bronchoalveolar lavage bal samples from hivnegative south african patients with pulmonary tuberculosis we additionally compared these clinical samples with in vitro log phase aerobic growth and hypoxic nonreplicating persistence nrp of m tuberculosis transcripts assayed in sputum and bal via multiplex rtpcr had a pvalue but none were significant after correction for multiple testing categorical enrichment analysis indicated that expression of the hypoxiaresponsive dosr regulon was higher in bal than in sputum m tuberculosis transcription in bal and sputum was distinct from both aerobic growth and nrp with a range of transcripts significantly differentially expressed T3 multiple testing correction collectively our results indicate that m tuberculosis transcription in sputum approximates m tuberculosis transcription in the lung minor differences between m tuberculosis transcription in bal and sputum suggested lower oxygen concentrations or higher nitric oxide concentrations in bal m tuberculosistargeted transcriptional profiling of sputa may be a powerful tool for understanding m tuberculosis pathogenesis and monitoring treatment responses in vivo | MEDAL |
polypharmacy is frequent in the elderly population and is associated with potentially drug inappropriateness and drugrelated problems | MEDAL |
mycoplasma hyopneumoniae the aetiological agent of porcine enzootic pneumonia regulates the presentation of proteins on its cell surface via endoproteolysis including those of the cilial adhesin p mhj these proteolytic cleavage events create PET adhesins that bind to proteoglycans and glycoproteins on the surface of ciliated and nonciliated epithelial cells and to the circulatory host molecule plasminogen two dominant cleavage events of the p preprotein have been previously characterized however immunoblotting studies suggest that more complex processing events occur these extensive processing events are characterized here the PET significance of the p cleavage fragments is also poorly understood AC using heparin fibronectin and plasminogen as bait and peptide arrays were used to expand our knowledge of the adhesive capabilities of p cleavage fragments and characterize a novel IB motif in the cterminus of p further we use immunohistochemistry to examine in vivo the biological significance of interactions between m hyopneumoniae and fibronectin and show that m hyopneumoniae induces fibronectin deposition at the site of infection on the ciliated epithelium our data supports the hypothesis that m hyopneumoniae possesses the molecular machinery to influence key molecular communication pathways in host cells | MEDAL |
to test whether there is a relationship between handedness and the intraocular pressure and there is a lateralization in the intraocular pressure the intraocular pressures of the RA and lefteyes were compared in RA and lefthanded students the intraocular pressures were higher in the righteye than in the lefteye in men righthanders and righteyed subjects there was no rightleft difference in females lefthanders lefteyed and botheyed subjects the intraocular pressure of RA and lefteyes was higher in lefthanders than righthanders and there were significant negative correlations between hand preference and the intraocular pressure of both RA and lefteyes we have concluded that the dominant eye has higher intraocular pressure compared to the nondominant one and there is a relationship between hand preference and the intraocular pressure | MEDAL |
orochol a live oral cholera vaccine licensed in switzerland and in other countries is based on the GM vibrio cholerae CS cvdhgr this strain is derived from the wildtype o CS inaba b by deletion of a fragment internal to the ctxa gene encoding the a S1 of cholera toxin and by replacement of an internal fragment of the hlya gene with a fragment carrying the mer operon mediating mercury resistance in this T0 we describe a PCR reaction pcr system for the PCD of wildtype Vc and the ID of the vaccine strain for the SQ control of production batches a multiplex pcr system that targets the intact ctxa gene of the wildtype CS and simultaneously the integration site of the mer operon in the hlya gene hlyamer of the vaccine strain cvdhgr was developed to evaluate the PCD limit of the system vaccine suspensions were artificially contaminated with wildtype v cholerae b cells and tested by pcr the PCD limit of the system was statistically evaluated and found to be at wildtype cells per vaccine sachet confidence limit this number is below the infective dose of wildtype Vc in switzerland this test is used in combination with other tests in the official batchrelease procedure to assure the safety of each batch of the cholera vaccine orochol | MEDAL |
a semiquantitative tintometric field kit has been used in the developing world for almost years to measure whole blood cholinesterase C2 in persons exposed to OPs the validity of this screening kit was evaluated among workers heavily exposed to organophosphates by comparison with a REF assay for erythrocyte cholinesterase overall correlation between the two methods was good however either sensitivity or specificity of the tintometric kit was less than for each of the three tintometric categories commonly used to define the limit of normal because baseline erythrocyte cholinesterase C2 were not available for this population the true sensitivity and specificity of the tintometric assay may be even lower | MEDAL |
decisions regarding patient care are made difficult because of lack of adequate knowledge surgeons try to decide upon choice of procedure and surgical technique based on the results of their experience and their understanding of published reports yet there are virtually no prospective controlled studies comparing different procedures for openangle glaucoma the present study compares peripheral iridectomy with a thermal sclerostomy as described by scheie with TE as described by watson in patients with primary openangle glaucoma a scheie procedure was performed in one eye and a trabeculectomy in the other the choice of procedure being determined randomly surgical technique was standardized shalloto result from a reduction in the number of IB sites it is concluded that brown AT mitochondria of preobese obob mice are more tightly coupled than those of normal siblings and that the activity of the thermogenic proton conductance pathway is lower in the mutant animals a decrease in thermogenesis in brown AT is therefore an early event in the OD of the obob mouse and precedes the appearance of obesity | MEDAL |
this T0 evaluated the effect of methylsulfonylmethane msm on carbon tetrachloride cclâinduced acute CL injury in rats a single injection of cclâ mlkg ip increased serum aminotransferases alt and ast activities in addition cclâ treatment led to elevation of hepatic MDA mda content as well as decrease in superoxide dismutase sod and catalase cat activities furthermore cytochrome p e cype content was suppressed while proinflammatory cytokines tumour necrosis factorα tnfα and interleukin il C2 increased in liver tissue after ccl administration we showed that acute cclâinduced damage was accompanied by a rise in baxbclâ ratio indicating apoptosis pretreatment with msm mgkg inhibited the increases of serum alt and ast activities decreased hepatic mda tnfα il and baxbclâ ratio compared to cclâ treated group on the other hand msm raised sod and cat activities as well as cype level in liver tissues the present study shows that msm possesses a hepatoprotective effect against cclâinduced CL injury in rats this protective effect might be through its antioxidant antiinflammatory and antiapoptotic properties | MEDAL |
under PB anesthesia arterial blood pressure and HR rate were recorded in rabbits the elevation in systemic blood pressure and increased HR rate induced by electrical stimulation of the RA thoracic sympathetic nerve trunk were depressed by either an i.v. of diltiazem microgramskg or by placing cotton pledgets soaked in mm diltiazem solution onto the right stellate ganglion these inhibiting effects of diltiazem were statistically significant with high SS frequencies hz but not when lower frequencies hz were used on the other hand hexamethonium mgkg iv or mm applied topically depressed cardiovascular responses to SS over a wide range of frequencies hz present results reveal a frequencydependent inhibition of ganglionic transmission by diltiazem and suggest that diltiazem may depress excessive sympathetic activity without affecting normal ganglionic transmission | MEDAL |
diabetes mellitus is the most common chronic disease in the world and a wide range of drugs including chinese herbs have been evaluated for the treatment of associated metabolic disorders this T0 investigated the potential hypoglycemic and renoprotective effects of an extract from the solidstate fermented mycelium of CS cs we employed the kkhij diabetic mouse model in which the mice were provided with a highfat diet for weeks to induce hyperglycemia followed by the administration of cs or rosiglitazone for consecutive weeks several parameters were evaluated including changes in body weight plasma lipid profiles oral gl tolerance tests insulin tolerance tests and plasma insulin concentrations our results show that the cs extract significantly elevated hdlldl ratios at weeks and decreased body WG at weeks interestingly cs treatment did not lead to obvious improvements in HG or resistance to insulin while in vitro mtt assays indicated that cs protects pancreatic beta cells against the toxic effects of stz cs also enhanced renal nka activity and reduced the accumulation of MM and collagen deposition in conclusion cs extract can potentially preserve βcell CF and offer renoprotection which may afford a promi by adjuvant chemotherapy but even in this prognostic favourable group long term survival is disappointing because of high local and distant failure rates postoperative chemoradiation has shown improved local control and overalls survival compared to SA but the value of additional radiation has been questioned in case of adjuvant chemotherapy however there remains a strong rationale for the addition of radiation therapy considering the high rates of microscopically incomplete resections after surgery as postoperative administration of radiation therapy has some GA disadvantages neoadjuvant and intraoperative approaches theoretically offer benefits in terms of dose escalation reduction of toxicity and patients comfort especially if hypofractionated regimens with highly conformal techniques like intensitymodulated radiation therapy are considered | MEDAL |
an epidemic outbreak of acute respiratory infection patients in an organized group of young people was observed in decembermay pneumococcal etiology was established by means of indirect immunofluorescence reaction in cases of outpatient pneumonia AB and acute respiratory diseases respiratory chlamydiosis caused by CT pneumoniae was detected in enzyme immunoassay with the use of immunocomb chlamydia bivalent igg in patients with pneumonia acute bronchitis and acute respiratory diseases synergic relationship between pneumococcal and chlamydial infections was noted | MEDAL |
theoretical considerations together with simulations of singleparticle cryo-EM images of biological assemblies in ice demonstrate that atomic structures should be obtainable from images of a few thousand asymmetric units provided the molecular weight of the whole assembly being studied is greater than the minimum needed for accurate position and orientation determination however with present methods of specimen preparation and current TSM and detector technologies many more particles are needed and the alignment of smaller assemblies is difficult or impossible only larger structures with enough signal to allow good orientation determination and with enough images to allow averaging of many hundreds of thousands or even millions of asymmetric units have successfully produced highresolution maps in this review we compare the contrast of exp electron cryomicroscopy images of two smaller molecular assemblies namely apoferritin and betagalactosidase with that expected from perfect simulated images calculated from their known xray structures we show that the contrast and signaltonoise ratio of exp images still require significant improvement before it will be possible to realize the full potential of singleparticle cryo-EM in particular although reasonably good orientations can be obtained for betagalactosidase we have been unable to obtain reliable orientation determination from exp images of apoferritin simulations suggest that at least fold improvement of the contrast in experimental images at å resolution is needed and should be possible | MEDAL |
we have recently demonstrated that sallylcysteine sac induces protection on neurochemical biochemical and behavioral markers of striatal damage in different neurotoxic animal models including a murine model induced by methylphenyltetrahydropiridinium mptp i.t. to mice indicating that prooxidant reactions underlie neurotoxicity in these models garcÃa et al in this work we investigated whether sac can protect the striatum of mice from the morphological alterations in the mptp toxic model and if this response is correlated with a reduction in Th1 cytokine TN factorα tnfα and iNOS synthase inos expressions and further reduction in astrocyte activation GFA protein gfap expression the striatal tissue from mptp injected animals mgkg ip à days showed a significant degree of cell damage and enhanced immunoreactivities to gfap tnfα and inos as well as an enhanced number of apoptotic nuclei treatment of mice with sac mgkg ip à days in parallel to mptp significantly reduced or prevented all these markers our results suggest that mptpinduced morphological alterations recruit a Th1 component triggered by cytokine tnfα release and nitric oxide formation which is CS to the antioxidant properties of sac this antioxidant is an ERP experimental tool to reduce the BB lesions associated with oxidative damage and inflammatory responses | MEDAL |
we report the results of the first prospective randomized multicenter T0 of immunosuppressive treatment in patients with previously UT nonsevere aplastic anemia aa as defined by a PMNL count of at least x l and transfusion dependence patients were randomized to receive cyclosporin csa CT or the combination of horse antithymocyte AHG atg lymphoglobuline merieux lyon france and csa the endpoint of the study was the hematologic response at months one hundred fifteen patients were randomized and assessable with a median followup period of months received csa and atg and csa in the csa group the percentage of complete and partial NR was and respectively for an overall response rate of a significantly higher overall response rate of was found in the atg and csa group with complete and partial responders p compared with csa alone the combination of atg and csa resulted in a significantly higher median hemoglobin level and platelet count at months fewer patients required a second course of treatment before months due to a nonresponse in the csa group of patients required a course of atg before months because of disease progression compared with only of in the atg and csa group the survival probabilities for the two groups were comparable csa group and atg and csa group but at days the prevalence of patients surviving free of transfusions which excluded patients requiring second treatment because of nonresponse death PD or relapse was in the csa group and in the atg and csa group p we conclude that the combination of atg and csa is superior to csa alone in terms of the hematologic response the quality of response and early mortality and a second course of immunosuppression is less frequently required | MEDAL |
the akt pathway is very important in both development and cancer here we show that expression of CS kinase i epsilon ckiepsilon causes upregulation of the akt pathway despite normal protein expression of the pathway inhibitor phosphate and tensin homologue deleted on chromosome ten pten conversely we show that a ckiepsilondeltaspecific inhibitor can inhibit akt phosphorylation at both thr and ser and drastically reduce phosphorylation of the akt target glycogen synthase kinase beta gskbeta these conclusions were confirmed between mcf cells transiently transfected with ckiepsilon and hst cells which already express endogenous ckiepsilon the results suggest that ckiepsilon is a new positive regulator of the akt pathway here we propose that rather than inhibiting pten function ckiepsilon positively regulates akt possibly by inhibiting protein phosphatase a ppa | MEDAL |
the formation of polycyclic aromatic hydrocarbon is a widespread issue during the supercritical water gasification of SS which directly reduces the gasification efficiency and restricts the technology practical application the changes of the concentrations and forms as well as the synthesis rate of polycyclic aromatic hydrocarbons in the residues from supercritical water gasification of dewatered sewage sludge were investigated to understand influence AF and the reaction pathways results showed that the increase of reaction temperature during the heating period favours directly concentration of polycyclic Ah especially highermolecularweight especially when it raise above °c lower heating and cooling rate essentially extend the total reaction time higher polycyclic Ah concentration and higher number of rings were generally promoted by lower heating and cooling rate longer reaction time and higher reaction temperature the lowermolecularweight polycyclic aromatic hydrocarbons can be directly generated through the decomposition of aromaticcontaining compounds in sewage sludge as well as ring and ring polycyclic aromatic HCs can be formed by aromatization of CS possible mechanisms of reaction pathways of supercritical water gasification of sewage sludge were also proposed | MEDAL |
liposuctionderived stem cells PLA have recently been shown to be capable of differentiating into bone most studies on osteoblastic growth and differentiation have been conducted in a conventional twodimensional SC system however in native bone OBs are situated in a threedimensional configuration there have been limited studies of processed lipoaspirate SMB in threedimensional systems the authors studied the influence a threedimensional scaffold has on the expression of genes related to osteogenesis and angiogenesis in processed lipoaspirate cells | MEDAL |
the transepidermal penetration and metabolic disposition of cbenzoyl peroxide were assessed in vitro excised human skin and in vivo Rh monkey in vitro the benzoyl peroxide penetrated into the skin through the stratum corneum or the follicular openings or both and was recovered on the dermal side as BA in vivo benzoic acid was recovered from urine in amounts equivalent to and of the radiolabel following respectively topical and IM administration of small amounts of cbenzoyl peroxide we conclude that benzoyl peroxide penetrates as such into the skin layers and is converted therein to benzoic acid which in turn is absorbed into the SVR circulation renal clearance of the metabolite is sufficiently rapid as to preclude its hepatic conjugation with glycine since following topical administration to Rh monkeys no hippuric acid was found in the urine as could have been expected had a significant amount of benzoic acid passed through the liver | MEDAL |
the light microscopic electron microscopic and histochemical features of a highly malignant colonic RT resected from a year old man are presented the tumor was composed predominantly of undifferentiated cells with focally admixed neuroendocrine exocrine and squamous cells occasionally arranged in an organoid manner histochemically the RT contained argyrophilic cells as well as cells that reacted positively with the antibodies to alphaantitrypsin alphaantichymotrypsin carcinoembryonic antigen and lysozyme the term SC carcinoma of the intestine is proposed for this highly malignant RT composed of undifferentiated cells exhibiting only focally their multidirectional developmental capacity | MEDAL |
secondary bone grafting from the IC is the gold standard in the reconstruction of maxillary AM bone defects in cleft patients numerous techniques for this graft have been described although none is considered clearly superior at this time a retrospective chart study was performed of alveolar clefts in patients who underwent an AM iliac crest bone VG with nasal floor reconstruction with or without concomitant AA cheilorhinoplasty fortyfour patients were included in the bone grafting group and in the concomitant cheilorhinoplasty group both CG had a sulcular translation mucoperiosteal flap with anterior repositioning of the gingival papilla as the preferred intraoral flap technique onethird of patients had undergone previous attempts at oronasal fistula closure results showed bergland stage in the cheilorhinoplasty group and in the bone graft group two failures stage were observed in the bone VG group the infection rate was in the cheilorhinoplasty group and in the bone graft group of clefts in the bone VG group showed postoperative residual fistulas none were observed in the cheilorhinoplasty group the sulcular translation flap is a simple surgical RPA and shows a low complication rate | MEDAL |
the oxidative phosphorylation system oxphos is organized in five multiprotein complexes comprising four complexes iiv of the RC and atp synthase complex v oxphos has a vital role in cellular energy metabolism and atp production enzyme analysis of individual oxphos complexes in a skeletal muscle biopsy remains the mainstay of the diagnostic process for patients suspected of mitochondrial cytopathy a fresh muscle biopsy is preferable to a frozen muscle biopsy because of the possibility to measure the overall capacity of the oxphos system in about of patients referred to our center for muscle biopsy reduced ATP oxidation rates and atp creatine phosphate production rates were found without any defect in complex iv and the pyruvate dehydrogenase complex in a subset of patients it is necessary to investigate fibroblasts for diagnostic purposes the indications for biochemical investigations in fibroblasts are a if no muscle sample is available b if prenatal diagnosis is required c to clarify the results obtained in muscle tissue if no clearcut diagnosis can be made d if moleculargenetic investigations are required e for research purposes fibroblasts are less suitable than fresh muscle for investigating respiratory chain disorders for the following reasons i a defect that is present in a muscle is not always expressed in fibroblasts ii exclusion of a defect in fibroblasts does not exclude the diagnosis with regard to muscle iii a specific DP of CA demonstrated in fibroblasts may not be reflected in muscle tissue iv enzyme deficiencies found in muscle are generally more pronounced than in fibroblasts an exact diagnosis of RC defects is a prerequisite for rational therapy and genetic counseling provided guidelines for specimen collection are followed there are now reliable methods for identifying respiratory chain defects | MEDAL |
plasma highdensity lipoprotein cholesterol hdlc level is a strong inverse predictor of cardiovascular disease cvd development tangier disease a consequence of mutations in the atp IB cassette transporter abca gene is associated with very low hdlc levels still the relationship between tangier disease and cvd is not always evident the T0 investigates usefulness of lipoprotein subfractions oxidative AS and paraoxonase pon status assessment for evaluation and management of patient with low hdlc phenotype | MEDAL |
the purpose of this work was to investigate the survival and intracellular growth of escherichia coli bacteriophages phi x t and t subjected to freezing down to degrees c the survival was for phage t for phage phi x and for phage t single growth cycles dna and protein biosynthesis did not change in phages phi x and t T3 freezing if bacteria were INF with phage t subjected to freezing the rates of phage dna and protein synthesis decreased the latent period took more time and phage yield decreased | MEDAL |
an immunocytochemical method using a TPS monoclonal antibody was employed to detect dna polymerase alpha in Dm embryos during the first nuclear division cycles after fertilization the antidna polymerase alpha antibody stained the ooplasm of the unfertilized egg indicating that dna polymerase alpha is maternally stored strong nuclear IF with the antibody over the weaker IF of the cytoplasm was observed at interphase throughout the nuclear division cycles the staining of the cytoplasmic regions surrounding the nucleus was much stronger than the other region of the syncytial cytoplasm until cycle although prophase nuclei were stained with the antibody metaphase chromosomes were never stained throughout the cycles the chromosomal nuclear IF reappeared at anaphase until cycle and at telophase in later cycles the staining of the syn cytoplasm except for the cortical region became faint by cycle suggesting the consumption of the maternal storage by this cycle these results suggest that dna polymerase alpha dissociates from chromosomes at the beginning of metaphase then in later MI phases it is transported from the syncytial cytoplasm into nuclei to participate in formation of the AS dna replication enzyme complex | MEDAL |
a rhodiumii dibenzotetramethylazaannulene dimer tmtaarh undergoes metathesis reactions with cpcrco cpmoco cpfeco coco and mnco to form tmtaarhm complexes m crcpco mocpco fecpco coco or mnco molecular structures were determined for tmtaarhfecpco tmtaarhcoμcoco and tmtaarhmnco by xray diffraction equilibrium constants measured for the metathesis reactions permit the estimation of several tmtaarhm bond dissociation enthalpies rhcr kcal mol rhmo kcal mol and rhfe kcal mol reactivities of the bimetallic complexes with synthesis gas to form tmtaarhcoh and mh are surveyed | MEDAL |
this review summarises the occurrence of herpesvirus infections in pinnipeds and data from investigations carried out by the authors these data demonstrate that herpesvirus isolates collected from harbour seals phoca vitulina linnaeus during the seal epidemic were almost indistinguishable from feline HHV-8 and that antarctic weddells seals leptonychotes weddelli lesson carry antibodies against seal HHV-8 from the northwestern europe seal epidemic the significance of herpesvirus infections in seals is discussed | MEDAL |
collagenase treatment of skeletal muscle results in the formation of large spheres of CM microns diam a procedure is described for purification and concentration of these giant membrane LDV prepared from rat muscle morphological observations marker enzyme analysis and immunoblotting demonstrate that the vesicles are of plasma membrane origin and that sarcoplasmic reticulum ttubules and mitochondrial inner CM are absent from the preparation western blots demonstrate that the LDV contain glut glucose transporters whereas glut could not be detected LDV prepared from control muscle display TPS transport of dglucose with a maximum velocity vmax for glucose influx of approximately pmolmg BPM proteins and an apparent michaelis constant km of mm measured at zerotrans conditions at room temperature muscle contractions in vivo doubled the vmax of vesicle GT and membrane glut content but did not change km in contrast in vivo administration of insulin did not affect vesicle GT or membrane glut content the combination of insulin and contractions caused similar changes as did contractions alone it is concluded that the present vesicle population contains membrane components almost exclusively derived from the BPM and contains very little if any glut but substantial amounts of glut thus the preparation allows the study of transport kinetics of pure glut transporters the procedure for preparing vesicles probably results in activation of the GT system similar to the activation by insulin but not by contractionsabstract truncated at words | MEDAL |
the pula gene encoding a novel TS type i pullulanase pula from bacillus sp cicim was identified from genomic dna the open reading frame of the pula gene was base pairs long and encoded a polypeptide pula of amino acids with a calculated molecular mass of da the pula gene was expressed in escherichia coli and bacillus subtilis recombinant pula showed optimal activity at ph and °c the enzyme demonstrated moderate thermostability as pula maintained more than of its acitivity when incubated at °c for h the enzyme could CR hydrolyze pullulan to maltotriose and hydrolytic activity was also detected with glycogen ST and amylopection but not with amylose which is consistent with the property of type i pullulanase pula may be suitable for industrial applications to improve the yields of fermentable sugars for bioethanol production | MEDAL |
the present research examined directional scanning effects dse as a CF of reading habit strength among right handers a picture array naming and recall task was administered to three groups of child readersunidirectional righttoleft readers of arabic unidirectional lefttoright readers of kannada and bidirectional readers of urdu and englishand one group of urdu illiterate adults the results showed a righttoleft dse in the arabic and urdu readers in the latter group the strength of the scanning effect decreased with greater schooling in english no rl effect was observed in the kannada readers or in the urdu illiterates these results extend prior research in documenting an invasion of culturally acquired reading scan habits onto a nonlinguistic domain it is suggested that directional scanning effects be controlled or else directly examined in future laterality research involving nonlinguistic stimuli | MEDAL |
to develop a novel quantitative eeg qeeg based analysis method cepstral distance cd and compare it to spectral distance sd in detecting eeg changes related to GI in rats | MEDAL |
tubulointerstitial changes in the diabetic kidney correlate closely with renal fibrosis and transforming growth factorbeta tgfbeta is thought to play a key role in this process in contrast HGF hgf has shown therapeutic effects on injured renal tubules in animal models this T0 was undertaken to test the hypothesis that the preventive effects of hgf may result from interventions in tgfbetamediated signaling and collagen iii secretion we examined the expression of hgfhgf receptor cmet and tgfbeta in renal fibroblasts at multiple time points the effects of rhHGF on tgfbeta expression were studied by rtpcr and WB and the C2 of collagen iii were measured by elisa in the highglucose condition the expression of hgf and cmet in renal fibroblasts was detected as early as hours following CC while the level of tgfbeta peaked at hours the addition of recombinant human hgf to the culture media dosedependently inhibited tgfbeta mrna expression and reduced collagen iii secretion by these results indicate that during hyperglycemia hgf inhibits tgfbeta signaling and type iii collagen activation in interstitial fibroblasts furthermore we should recognize that changes in the balance between hgf and tgfbeta might be decisive in the path +dP/dt cell adhesion and CSK dynamics the possible influence of mechanical effects of cell motility on emerging of calcium signal is also discussed | MEDAL |
our primary objective in this study was to determine the differences in inflammatory profile between patients with high risk for obstructive SA osa versus low risk in a patient population undergoing cardiac rehabilitation and to evaluate the effect of weeks of rehabilitation on cytokine C2 in the groups a total of consecutive patients were screened using the berlin questionnaire to determine high and low risk of osa of the total consecutive patients patients did not consent to participate and did not get cytokine analysis of the remaining patients men and women patients were found to have high risk for osa as predicted by the berlin questionnaire men predominated the highrisk group there was a trend of increased cytokine load in the highrisk group however it did not reach statistical significance except for monocyte chemoattractant protein p value a significant reduction was noted in cytokine C2 T3 weeks of rehabilitation in the group with osa which was statistically significant p value the highrisk group patients had elevated cytokine levels especially monocyte chemoattractant protein further a significant reduction in cytokine levels was noted in the highrisk group after weeks of rehabilitation | MEDAL |
CTS is a rare but lifethreatening obstructive airway disease it usually presents in early infancy and requires surgical intervention this T0 is a review of our experience in the management of congenital tracheal stenosis in children at kk womens and childrens hospital singapore | MEDAL |
psychophysical and EP studies indicated that the umami substances have no enhancing activity on other primary tastes experiments using amiloride clearly show that the umami component of canine CT nerve response to umami substances is independent of the salt component single fiber analysis of the responses of the mouse glossopharyngeal nerve and the monkey primary taste cortex neuron show that the responses to umami substances are independent of other primary tastes a C1 synergism between monosodium glutamate msg and disodium inosinate imp or disodium guanylate gmp is observed in dogs and is explained in terms of allosteric effect the order of intensity of umami taste induced by a mixture of mm gmp and mm of various agonists for the glutamate receptors was glu ibotenate dlaminophosphonobutyric acid dlap aminocyclopentanetransdicarboxylic acid transacpd kainate nmethyldaspartate nmda and rsaminohydroxymethylisoxazolepropionic acid ampa which are agonists for ionotropic receptors have no umami taste it was concluded that the umami receptor is not identical to any of known glutamate receptors and there seems to be a unique receptor for umami | MEDAL |
objects seen from unusual relative to more canonical views require more time to categorize and recognize and according to object model verification theories additionally recruit prefrontal processes for cognitive control that interact with parietal processes for MR to test this using PET magnetic resonance imaging people categorized and recognized known objects from unusual and canonical views canonical views G1 some components of a default network more on categorization than recognition activation to unusual views showed that both VP and dorsal visual pathways and prefrontal SC have key roles in visual object constancy unusual views activated objectsensitive and mental rotation and not saccade regions in ventrocaudal intraparietal transverse occipital and IT sulci and VP PM cortex for verification processes of MM testing on any task a collaterallingual sulci place area activated for mental rotation WM and unusual views on correct recognition and categorization trials to accomplish detailed spatial matching ventrolateral prefrontal cortex and objectsensitive lateral occipital sulcus activated for mental rotation and unusual views on categorization more than recognition supporting verification processes of MM prediction this visual knowledge framework integrates vision and memory theories to explain how distinct prefrontalposterior networks enable meaningful interactions with objects in diverse situations | MEDAL |
the authors describe a rare case of amyloidosis in a female patient suffering from periodic disease pd for years without any clinicolaboratory signs of renal impairment but with marked clinical malabsorption cachexia endoscopic xray and other manifestations of gastrointestinal amyloidosis this case is of interest since patients suffering from amyloidosis due to pb develop malabsorption very rarely namely in of cases as a rule it develops in patients with pronounced chronic renal failure on hemodialysis or with a history of kidney transplantation in this particular case the patient demonstrated selective marked damage to the gastrointestinal tract with the kidneys remaining practically IN a possibility of the indicated variety of amyloidosis should be considered in specification of the genesis of persistent diarrhea in pb patients | MEDAL |
the aim of this T0 was to compare wound complications from vy flap vs primary closure in the setting of abdominoperineal resection | MEDAL |
a yearold woman was admitted to our hospital because of palpitation dyspnea and anterior chest discomfort she had had a heart murmur since childhood on physical examination a Rg grade ejection systolic murmur was maximally audible at the second left intercostal space with a systolic thrill the chest xray film showed the cardiothoracic ratio of and the electrocardiogram demonstrated right ventricular hypertrophy calcification of the tricuspid annulus was recognized by chest xray films anteroposterior and lateral views mmode and crosssectional echocardiograms and the computed tomogram ct of the chest cardiac catheterization revealed the presence of valvular PS and atrial septal defect these findings were confirmed by surgical T0 since the first description of paktovskii in nine cases of calcification of the tricuspid annulus were reported but no case has been described in the japanese literature contrary to the MA the echocardiographic and computed tomographic features of the calcification of the TA has not been reported previously in this paper we reported a middle aged patient with circumferential calcification of the TA in association with severe PS and atrial septal defect and the features of the echocardiogram and ct images were illustrated | MEDAL |
arylsulfatase preferentially present in the human eosinophil as compared to other WBCs was isolated by sequential GF and cation exchange chromatography the apparent molecular weight of the preferential cleavage of nitrocatechol sulfate pncs over pacetylbenzenesulfonic acid pabs inhibition by phosphate ions and ph optimum of are characteristics of a type ii b arylsulfatase eosinophil arylsulfatase inactivated purified human slow reacting substance of anaphylaxis srsa in a timedependent reaction with the rate dependent upon the enzymesubstrate ratio that srsa inactivation was the result of intrinsic arylsulfatase activity was indicated by association of pncs cleavage and srsa inactivating activity during chromatography the similar ph optimum for cleavage of both substrates and the capacity of srsa to inhibit pncs cleavage by arylsulfatase the finding that eosinophil arylsulfatase inactivates srsa suggests that eosinophil ingress into the site of an immediate HSR in response to ecfa could represent a RII CF | MEDAL |
in dogs with chronic hypochloremic metabolic alkalosis associated with ecfv contraction plasma hco hcop normalizes during expansion of ecfv with a solution containing cl and hco in concentrations duplicating those in the plasma before expansion isometric expansion the kidney selectively rejects po hco and retains cl if this preferential cl less than hco reabsorptive selectivity were a characteristic renal response to ecfv expansion isometric expansion during hyperchloremic acidosis would exacerbate the acidbase disturbance rather than correct it as it does in alkalosis we examined the effect of ISO expansion in dogs with chronic hyperchloremic MA induced by hcl feeding or mineralocorticoid hormone deficiency during expansion as the expected decrease occurred in the fractional reabsorption of na a lesser decrease occurred in fractional reabsorption of hco whereas a greater decrease occurred in fractional reabsorption of cl the kidney selectively retained administered hco and rejected cl hcop normalized the shift to bicarbonateselective from chlorideselective anion reabsorption during ecfv expansion in metabolic acidosis vs metabolic alkalosis indicates that in response to ecfv expansion the kidney selectively alters the ratio of bicarbonate to chloride concentration in the tubular reabsorbate in the direction that tends to normalize plasma acidbase composition irrespective of the direction of deviation of the initial plasma bicarbonate concentration the signal that initiates the shift in anion reabsorptive selectivity remains to be identified | MEDAL |
electrographic and clinical observations were made as long as months after the injection of ibotenic acid ibo solution micrograms in microliter of PBS through a chronically implanted cannula into unilateral amygdala of freely moving and nonanesthetized cats the control group PB group showed no NC during the observation period about to T2 T3 the i.t. of ibo focal AM seizures occurred and propagated to the adjacent limbic structures clinically attention and ipsilateral mydriasis were observed the seizures occurred only to times and ceased within hours cats became electroclinically normal afterwards histopathological examination revealed a small CN at the injected site of the amygdala remarkable pyknosis and gliosis were noted around the necrosis remote lesions such as neuronal cell loss and pyknosis were observed in the ipsilateral pyramidal cell SL of the hippocampus but these changes were mild and not so severe as compared to our previous report of KA microinjection in cats authors emphasized that ibo should be an excellent tool for lesion making and also suggested that an aseptic manipulation was essential in the lesion T0 in cats | MEDAL |
comparisons were made between ultrasound behavioural recordings of healthy term fetuses and recordings of the same variables of the same individuals at to days postnatally statistical comparison of the frequency of eye movements limb and BMs revealed that the fetal states f and f were comparable to quiet sleep s and rapid EM rem sleep s respectively in the P0 comparisons between the fetal states and wakefulness were less clearcut the HR rate in each of the three states in the fetus was about bpm higher than in the corresponding postnatal state although the patterns were similar states f and f accounted for a higher percentage of the fetal observations than states s and s in the neonatal observations but the ratio of theis progresses into one form of SMD and that SMD is partly genetically determined | MEDAL |
it has been suggested previously that the increased litter size of the prolific MS pig may result from an increased littermate embryonic synchrony this T0 compared embryonic diversity in meishan and domestic white L1 F1 sows thirdparity meishan sows n and secondparity domestic sows n were observed for estrus every h and were handmated at and h after first observed estrus d to boars of the same breed the sows were slaughtered on d mean sem embryos were flushed from each uterine horn and were individually sonicated lyophilized and frozen degrees c until they were assayed for estradiol beta e beta dna and protein content ovulation rate was higher p in meishan than in domestic sows the average littermate embryonic diameter was smaller p for meishan than for domestic sows although morphological embryonic diversity sigma for diameter did not differ p between the two breeds in addition for embryos of the same diameter no differences p in number of embryonic cells micrograms of dnaembryo or in cell size proteindna ratio were observed for either breed the more T3 mm embryos from meishan sows contained less p e beta picogramsembryo than did embryos of the same size from domestic sows the prolificacy of the meishan sow may result from both an increased or and reduced e beta production by meishan embryosabstract truncated at words | MEDAL |
moricizine is a class i antiarrhythmic drug in preclinical studies it produces a concentrationdependent decrease in the Vmax of phase depolarization speeds repolarization of phases and and decreases the action potential duration and ERP refractory period duration in cardiac purkinje SF it has no effect on the Mw of phase depolarization but suppresses normal automaticity in vitro and in vivo and suppresses abnormal automaticity in depolarized purkinje fibers also it suppresses early afterdepolarizations delayed afterdepolarizations and triggered activity in patients moricizine has minimal effects on the normal SN slows conduction in the atrium AVN hispurkinje system and VVI myocardium and has little effect on the atrial and VVI refractoriness the intensity of moricizine action on the atrioventricular node HPS and jt interval are doserelated coadministration of digoxin and moricizine intensified the lengthening of the pr ah and hv intervals and produced more shortening of the jt interval patients in whom moricizine was efficacious had a significantly greater lengthening of the ah and qrs intervals than those in whom moricizine was not efficacious in some patients with SND moricizine produced SB increased SN REC time and produced seconddegree or complete sinoatrial block | MEDAL |
we have evaluated over a mean follow up period of sd years the lung function in a group of workers according to the presence workers or absence workers of PP at chest rxfilmshrct a generalized estimating equation gee approach was used to investigate the relation between the loss of pulmonary function and i presenceabsence of pleural plaques ii smoking status and iii work seniority in work places with exposure to asbestos the mean age at the first examination was sd years and work seniority sd years multivariate gee RPA to age and heightadjusted spirometric data measures of vc fev and determinations of tlc showed that pleural plaques were not associated with significant loss of pulmonary CF sm or py when compared with nosmokers showed significant loss of vc p fev p and tlc p an occupational history in work places with SE to asbestos ship buildingrepairing was significantly associated with a slight but significant p year decrease in vc and fev | MEDAL |
while PVI pvi is an ERP curative procedure for patients with atrial fibrillation af BPV pv stenosis is a potential complication which may lead to PS that are often unrecognized the aim of this T0 was to compare differences between ablation CS in pulmonary venous flow pvf measured by transthoracic doppler echocardiography tte before and T3 pvi | MEDAL |
heme oxygenase ho catalyzes the first and ratelimiting step in the metabolism of free heme into equimolar amounts of ferrous iron carbon monoxide co and BV BV is subsequently converted to bilirubin by biliverdin reductase ho has recently been identified as a promising therapeutic target in the treatment of vascular inflammatory disease including atherosclerosis ho represses inflammation by removing the Th1 molecule heme and by generating co and the bile pigments biliverdin and bilirubin these ho MRP are capable of blocking innate and adaptive immune responses by modifying the activation differentiation maturation andor polarization of numerous immune cells including EC cells monocytesmacrophages dendritic cells t lymphocytes mast cells and platelets these cellular actions by co and bile pigments result in diminished leukocyte recruitment and infiltration and Th1 mediator production within AS lesions this review highlights the mechanisms by which ho suppresses V1 inflammation in atherosclerosis and explores possible therapeutic modalities by which ho and its MRP can be employed to ameliorate vascular inflammatory disease | MEDAL |
current standard treatment regimens for helicobacter pylori infection provide eradication rates to these rates have been achieved with a variety of week triple therapies using two antibiotics and an acid suppressant antibiotic resistance which may develop during failed treatment is becoming increasingly common and has led to studies of new regimens for primary therapy and new strategies for salvage of failed therapy other regimens have been designed and tested with the aim of decreasing the cost of initial therapy or to improve compliance but abbreviated regimens have high incidence of failure and may add to the problem of resistance increasing attention has been paid to the need for and timing of the determination of antibiotic resistance of h pylori isolates either at the time of initial diagnosis or after treatment failure new simpler and noninvasive methods are offered for followup to determine if eradication has been successful treatment regimens should be chosen based on local drug susceptibility patterns and the availability of approved therapeutic agents in each country established indications for testing for h pylori and administering therapy include active or inactive peptic UD mucosaassociated LT malt lymphoma as well as hyperplastic polyps hyperplastic gastropathy postendoscopic resection for gastric malignancy and acute h pylori gastritis it is now largely accepted that noninvestigated dyspepsia is an indication for testing for and treating h pylori but that dyspeptic symptod that although the transgenic lines possessed different types of integration events no unintended effects were identified however we found that the majority of genes showing differential expression were identified as stressrelated genes and that environmental conditions had a C1 impact on the expression of several genes proteins and metabolites we suggest that the microarray approach has the potential to become a useful tool for screening of unintended effects but state that it is crucial to have substantial information on the natural R2 in traditional crops in order to be able to interpret omics data correctly within the framework of food safety assessment strategies of novel plant varieties including genetically modified plant varieties | MEDAL |
gadolinium gdcontaining microcapsules designed for neutroncapture therapy nct were prepared by a spouted bed coating process microcapsules were designed as a gdreservoir they were prepared with the following properties particle size was smaller than microns gdcontent was as high as possible and release of gd was suppressed as long as possible calcium carbonate microns was selected as a speed particle as a gdsource gadolinium diethylenetriaminepentaacetic acid gddtpa or a synthesized waterinsoluble gddtpa derivative gddtpadistearylamide gddtpasa was layered onto the seed particles the releasesuppressing layer was composed of AQ acrylic latex of polyethyl acrylatemethyl methacrylatehydroxyethyl methacrylate in preliminary studies gddtpa microcapsules with microns mass median diameter were prepared they released gd with a short lagtime and hprolongation complete PR suppression was however difficult to achieve because of high watersolubility of gddtpa hence a hydrophobic derivative gddtpasa was next used as a gd source gddtpasa microcapsules could be prepared with a mass median diameter of microns gddtpasa content of the microcapsules was and release of gd was suppressed to less than over d | MEDAL |
a new protein found at sites of cellsubstrate adhesion has been identified by analysis of a nonimmune rabbit serum by indirect immunofluorescence this SS stains focal contacts adhesion plaques and the associated termini of actin filament bundles in cultured chicken cells western immunoblot analysis of total chick embryo fibroblast protein demonstrated an kd polypeptide to be the major protein recognized by the UF SS this kd protein is immunologically distinct from other known adhesion plaque proteins such as vinculin talin alphaactinin and fimbrin antibody affinitypurified against the electrophoretically isolated nitrocellulosebound kd protein retained the ability to stain the area of the adhesion PI which confirms that the kd protein is indeed a constituent of the focal contact the kd polypeptide has a basic isoelectric point relative to actin and fibronectin and it appears to be very low in abundance the kd protein is ubiquitous in chicken embryo tissues however it appears to be more abundant in fibroblasts and smooth muscle than in brain or CL intermediate C2 of the protein were detected in skeletal and cardiac muscle the subcellular CSD of the kd protein raises the possibility that this polypeptide is involved in linking AFs to the plasma membrane at CS of ATP att or regulating these dynamic interactions | MEDAL |
patterning in plants requires defining boundary domains that separate and organize the development of the neighboring organs two papers now show how the interplay between brassinosteroid phytohormones and frontier genes contributes to boundary formation in plants | MEDAL |
particle dissolution within macrophage phagolysosomes is hypothesized to be an important source of dissolved beryllium for input to the cellmediated immune reaction associated with OD of beryllium sensitization and CBD cbd to better understand the dissolution of beryllium materials associated with elevated prevalence of sensitization and cbd singleconstituent beryllium oxide beo particles sampled from a screener T0 finished product beo powder finish product beryllium metal powder and multiconstituent particles sampled from an arc furnace during processing of copperberyllium alloy aerosol materials were studied dissolution rates were measured using phagolysosomal simulant fluid psf in a static dissolution technique and then normalized to measured values of specific surface area to calculate a chemical dissolution rate constant k for each material values of k in gcm day for screener beo particles x and for beo powder x were similar p the value of k observed for beryllium metal powder x was significantly greater than observed for the beo materials p for arc furnace particles k x was significantly greater than observed for the beo materials p despite the fact that the chemical form of beryllium in the aerosol was beo these results suggest that dissolution of beryllium differs among physicochemical forms of beryllium and direct measurement of dissolution is needed for multiconstituent aerosol additional studies of the dissolution behavior of beryllium materials in a variety of mixture configurations will aid in developing exposureresponse models to improve understanding of the risk of beryllium sensitization and cbd | MEDAL |
bischloroethylnitrosourea bcnu and chloroethylcyclohexylnitrosourea ccnu induced dominant lethal and specificlocus mutations in male mice for both compounds the germ cell stage CS to the induction of DLM was dose dependent a dose of mg bcnu per kg bwt induced DLM primarily in spermatocytes whereas higher doses of bcnu induced dominant lethals in spermatids and SPC following doses of and mg ccnu per kg bwt dominant lethals were induced in spermatids and SPC similar to the results for higher doses of bcnu higher dose SE to bcnu and ccnu was associated with dominant lethals expressed as preimplantation loss reduction in total number of implants in addition higher doses of ccnu showed a cytotoxic effect in differentiating SPG both compounds induced specificlocus mutations in postspermatogonial germ cell stages of mice however ccnu increased also the specificlocus mutation frequency in spermatogonia in two out of three experiments we conclude in analogy with criteria developed by iarc that bcnu and ccnu are potential human mutagens | MEDAL |
horseradish peroxidase hrp tracer was applied to the gingival sulcus of implants or natural teeth at or mgml to investigate the sealing capacities of the periimplant epithelium pie and junctional epithelium je the extent of hrp penetration was observed under electron microscopy a tialv implant was inserted either immediately immediate implantation or weeks delayed implantation T3 extraction of the maxillary left first molar of rats the je of the right molar was used as a control although the whole pie of undecalcified sections appeared to be attached to the implant surface electron microscopically the internal basement lamina ibl and hemidesmosomes were deficient in the coronalmiddle region of the pie there were extensive extracellular deposits of hrp in the intercellular spaces between pie cells and hrp was blocked to some extent by the lamina lucida and lamina densa of the external BL and basal cell junction hrp was detected in the connective tissue under the pie but was not found in the connective tissue under the je intracellularly hrp was found in the LDV and granules of pie cells and je cells these were fewer in number in pie cells than in je cells there were no differences between the findings for immediate and delayed implantation the results indicate that a deficiency in the ibl permitted penetration of hrp from the gingival sulcus into the connective tissue under the pie and suggest that the endocytotic capacity of pie cells is inferior to that of je cells | MEDAL |
thyroid carcinoma is the most common malignancy of the endocrine organs although the majority of thyroid cancer patients experience positive outcomes ATC is considered one of the most aggressive malignancies current therapeutic regimens do not confer a significant survival benefit and new therapies are urgently needed oncolytic herpes simplex virus ohsv may represent a promising therapy for CA in the present T0 we investigated the therapeutic effects of a thirdgeneration hsv vector gδ on various human thyroid carcinoma cell lines in vitro two subcutaneous sc models of anaplastic thyroid carcinoma were also established to evaluate the in vivo antitumor tau of gδ | MEDAL |
dna barcode REF libraries linked to voucher specimens create new opportunities for highthroughput ID and taxonomic reevaluations this study provides a dna barcode library for about of the recognized species ofy incontinence mri demonstrated an extradural ovoid mass compressing the lumbar spinal cord surgical excision of the mass was performed histologically the mass was consistent with hemangiosarcoma with no involvement of the adjacent vertebrae the dog underwent a doxorubicinbased chemotherapy protocol with the addition of oral cyclophosphamide T3 completion of chemotherapy the dog was evaluated q mo for restaging clinicopathological evidence of primary tumor recurrence or metastatic disease was not detected for mo T3 initial diagnosis and treatment to the authors knowledge this is the first report of a primary extradural hemangiosarcoma in the lumbar vertebral column in a dog the clinical presentation diagnosis treatment and outcome are also discussed | MEDAL |
obsessivecompulsive disorder ocd is a disabling disorder that can be successfully treated however a high percentage of sufferers neither ask for nor receive treatment for their symptoms or they delay seeking treatment the AF underlying the treatmentseeking behaviour of ocd patients are still not clear this review includes studies published before april that analyse the possible variables related to the delayed helpseeking behaviour of ocd patients studies showed that individuals who asked for help were more impaired and reported poorer quality of life helpseeking behaviour was associated with greater insight severity TPS OCS such as aggressive and other unpleasant obsessions and comorbidity common barriers to seeking treatment were shame about the symptoms or about asking for treatment not knowing where to find help or inconveniences associated with treatment inconsistencies among the reviewed studies higr during the IET oxygen consumption carbon dioxide production and ventilatory equivalent were measured using a breathbybreath gas analyzer systolic blood pressure and heart rate were measured to calculate the DP from which doubleproduct breakpoint was determined salivary αamylase was measured to calculate the salivary threshold results oneway anova revealed no significant differences among workloads at the anaerobic threshold doubleproduct breakpoint and salivary threshold significant correlations were found between anaerobic threshold and salivary threshold and between anaerobic threshold and doubleproduct breakpoint conclusion as a method for estimating anaerobic threshold salivary threshold was as good as or better than determination of doubleproduct breakpoint because the rho between anaerobic threshold and salivary threshold was higher than the correlation between anaerobic threshold and doubleproduct breakpoint therefore salivary threshold is a useful index of anaerobic threshold during an incremental workload | MEDAL |
numerical estimation was used to determine adsorption isotherm parameters of a singlecomponent in a normalphase system the distribution isotherm of methyl deoxycholate was described between the mobile phase containing hexane EA methanol with varied concentration and a silica gel adsorbent the effect of the mobile phase composition on the isotherm parameters and the band profiles was investigated the results obtained were used to sjuxtaposed to the residue proposed to CF as general base catalyst CS homology and mutagenesis approaches have suggested several residues that may potentially function in rup binding not all of these proposed rup binding residues are closely juxtaposed in the structure of unliganded prk mechanistic approaches have been employed to investigate the amino acids which influence km rup and identify those amino acids most directly involved in rup IB prk is one member of a family of phospho or sulfo GOT proteins which exhibit a nucleotide monophosphate kinase fold structurefunction correlations elucidated for prk suggest analogous assignments for other members of this family of proteins | MEDAL |
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