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|---|---|---|---|---|---|---|---|
I recommend acceptance for publication in Life Sciences journal.
| null | null |
The idea to use whole genome data for archaeal phylogenies is interesting, as the 16S rRNA phylogenies can be poor in resolving relationships among archaeal lineage due to GC content bias in hyperthermophilic Archaea.
| null | null |
life5010949_makarova
| 0 |
Though a dissimilarity measure figures in the CVTree algorithm, it is not realistic to delineate taxa by using this measure at least for the time being.
| null | null |
I recommend acceptance for publication in Life Sciences journal.
| null | null |
life5010949_makarova
| 0 |
Though a dissimilarity measure figures in the CVTree algorithm, it is not realistic to delineate taxa by using this measure at least for the time being.
| null | null |
Round 1: and Author Response Summary Zuo et al.
| null | null |
life5010949_makarova
| 0 |
Perhaps an additional discussion point would be the advantages of using CVtree approach with regular concatenated proteins.
| null | null |
Evaluation I found major concerns along this manuscript, and suggest a substantial revision.
| null | null |
life5010949_makarova
| 0 |
However, I am personally not convinced that whole genomes bring additional advantages, or if we are better off by just using a conserved set of proteins.
| null | null |
present a comparative analysis of the taxonomic classification of the Archaea domain.
| null | null |
life5010949_makarova
| 0 |
Here monophyly must be understood in a pragmatic way restricted to the given set of input data and the reference taxonomy.
| null | null |
The idea to use whole genome data for archaeal phylogenies is interesting, as the 16S rRNA phylogenies can be poor in resolving relationships among archaeal lineage due to GC content bias in hyperthermophilic Archaea.
| null | null |
life5010949_makarova
| 0 |
The idea to use whole genome data for archaeal phylogenies is interesting, as the 16S rRNA phylogenies can be poor in resolving relationships among archaeal lineage due to GC content bias in hyperthermophilic Archaea.
| null | null |
present a comparative analysis of the taxonomic classification of the Archaea domain.
| null | null |
life5010949_makarova
| 0 |
For example, it cannot be expected that the same degree of dissimilarity may be used to delineate classes in all phyla.
| null | null |
R13 Round 2: and Author Response Authors have substantially improved the article, including language corrections, and have provided extensive clarifications to all initial criticisms.
| null | null |
life5010949_makarova
| 0 |
Perhaps an additional discussion point would be the advantages of using CVtree approach with regular concatenated proteins.
| null | null |
present a comparative analysis of the taxonomic classification of the Archaea domain.
| null | null |
life5010949_makarova
| 0 |
However, I am personally not convinced that whole genomes bring additional advantages, or if we are better off by just using a conserved set of proteins.
| null | null |
General Comments The topic is relevant for microbial taxonomy.
| null | null |
life5010949_makarova
| 0 |
About 180 archaeal genomes were used to calculate a new tree topology using CVTree.
| null | null |
Though a dissimilarity measure figures in the CVTree algorithm, it is not realistic to delineate taxa by using this measure at least for the time being.
| null | null |
life5010949_makarova
| 0 |
I recommend acceptance for publication in Life Sciences journal.
| null | null |
General Comments The topic is relevant for microbial taxonomy.
| null | null |
life5010949_makarova
| 0 |
It is the only described representative of a newly proposed phylum Nanoarchaeota and it cuts into the otherwise monophyletic phylum Euryarchaeota.
| null | null |
It has been known that tiny genomes of endosymbiont microbes often tend to move towards baseline of a tree and distort the overall picture.
| null | null |
life5010949_makarova
| 0 |
It is the only described representative of a newly proposed phylum Nanoarchaeota and it cuts into the otherwise monophyletic phylum Euryarchaeota.
| null | null |
I recommend acceptance for publication in Life Sciences journal.
| null | null |
life5010949_makarova
| 0 |
The tree was compared with several 16S rRNA trees reported in the literature, and the differences were minor.
| null | null |
The tree was compared with several 16S rRNA trees reported in the literature, and the differences were minor.
| null | null |
life5010949_makarova
| 0 |
I recommend acceptance for publication in Life Sciences journal.
| null | null |
present a comparative analysis of the taxonomic classification of the Archaea domain.
| null | null |
life5010949_makarova
| 0 |
However, I am personally not convinced that whole genomes bring additional advantages, or if we are better off by just using a conserved set of proteins.
| null | null |
Though a dissimilarity measure figures in the CVTree algorithm, it is not realistic to delineate taxa by using this measure at least for the time being.
| null | null |
life5010949_makarova
| 0 |
R13 Round 2: and Author Response Authors have substantially improved the article, including language corrections, and have provided extensive clarifications to all initial criticisms.
| null | null |
About 180 archaeal genomes were used to calculate a new tree topology using CVTree.
| null | null |
life5010949_makarova
| 0 |
The idea to use whole genome data for archaeal phylogenies is interesting, as the 16S rRNA phylogenies can be poor in resolving relationships among archaeal lineage due to GC content bias in hyperthermophilic Archaea.
| null | null |
Round 1: and Author Response Summary Zuo et al.
| null | null |
life5010949_makarova
| 0 |
The latter can be assigned only after comparison with a reference taxonomy which is not a rigid framework but a modifiable system.
| null | null |
Though a dissimilarity measure figures in the CVTree algorithm, it is not realistic to delineate taxa by using this measure at least for the time being.
| null | null |
life5010949_makarova
| 0 |
Here monophyly must be understood in a pragmatic way restricted to the given set of input data and the reference taxonomy.
| null | null |
Even if defined in the future, it must be lineage-dependent.
| null | null |
life5010949_makarova
| 0 |
It is the only described representative of a newly proposed phylum Nanoarchaeota and it cuts into the otherwise monophyletic phylum Euryarchaeota.
| null | null |
It is the only described representative of a newly proposed phylum Nanoarchaeota and it cuts into the otherwise monophyletic phylum Euryarchaeota.
| null | null |
life5010949_makarova
| 0 |
The measurement itself is novel, but same methods and theories have been reported in the authors’ previous papers.
| null | null |
The measurement itself is novel, but same methods and theories have been reported in the authors’ previous papers.
| null | null |
life5021101_makarova
| 0 |
Second Round of Evaluation Round 2: and Author Response It is also not clear about the “thermally induced shape fluctuations”, such as the temperatures and incubation length and etc., especially this is a hyperthermophilic archaeal membrane vesicle, which would be expected to be more thermostable than those eukaryotic ones.
| null | null |
Stroboscopic illumination measurements of GVs and theoretical models about shape fluctuation of GVs revealed that the archaeal lipid-based GVs showed the almost same value of bending elasticity modulus with synthetic lipid-based GVs.
| null | null |
life5021101_makarova
| 0 |
I also have questions and comments for acceptance.
| null | null |
Second Round of Evaluation Round 2: and Author Response It is also not clear about the “thermally induced shape fluctuations”, such as the temperatures and incubation length and etc., especially this is a hyperthermophilic archaeal membrane vesicle, which would be expected to be more thermostable than those eukaryotic ones.
| null | null |
life5021101_makarova
| 0 |
The measurement itself is novel, but same methods and theories have been reported in the authors’ previous papers.
| null | null |
The measurement itself is novel, but same methods and theories have been reported in the authors’ previous papers.
| null | null |
life5021101_makarova
| 0 |
Stroboscopic illumination measurements of GVs and theoretical models about shape fluctuation of GVs revealed that the archaeal lipid-based GVs showed the almost same value of bending elasticity modulus with synthetic lipid-based GVs.
| null | null |
However, the comment of the reviewer is notable and we state in the conclusion of the manuscript (Lines 229–232) that at room temperature the thermophilicity is not necessarily related to the bending elasticity.
| null | null |
life5021101_makarova
| 0 |
The measurement itself is novel, but same methods and theories have been reported in the authors’ previous papers.
| null | null |
The measurement itself is novel, but same methods and theories have been reported in the authors’ previous papers.
| null | null |
life5021101_makarova
| 0 |
I also have questions and comments for acceptance.
| null | null |
I also have questions and comments for acceptance.
| null | null |
life5021101_makarova
| 0 |
I also have questions and comments for acceptance.
| null | null |
According to the authors, this is the first report for the measurement of bending elasticity modulus for archaeal lipid-based GVs.
| null | null |
life5021101_makarova
| 0 |
The measurement itself is novel, but same methods and theories have been reported in the authors’ previous papers.
| null | null |
The measurement itself is novel, but same methods and theories have been reported in the authors’ previous papers.
| null | null |
life5021101_makarova
| 0 |
According to the authors, this is the first report for the measurement of bending elasticity modulus for archaeal lipid-based GVs.
| null | null |
The observed fluctuations of the membrane do not destabilize it.
| null | null |
life5021101_makarova
| 0 |
Stroboscopic illumination measurements of GVs and theoretical models about shape fluctuation of GVs revealed that the archaeal lipid-based GVs showed the almost same value of bending elasticity modulus with synthetic lipid-based GVs.
| null | null |
I also have questions and comments for acceptance.
| null | null |
life5021101_makarova
| 0 |
Round 1: and Author Response This manuscript described the measurement of bending elasticity of giant vesicles (GVs) prepared by archaea-derived lipids.
| null | null |
I also have questions and comments for acceptance.
| null | null |
life5021101_makarova
| 0 |
Round 1: and Author Response This manuscript described the measurement of bending elasticity of giant vesicles (GVs) prepared by archaea-derived lipids.
| null | null |
Second Round of Evaluation Round 2: and Author Response It is also not clear about the “thermally induced shape fluctuations”, such as the temperatures and incubation length and etc., especially this is a hyperthermophilic archaeal membrane vesicle, which would be expected to be more thermostable than those eukaryotic ones.
| null | null |
life5021101_makarova
| 0 |
Round 1: and Author Response This manuscript described the measurement of bending elasticity of giant vesicles (GVs) prepared by archaea-derived lipids.
| null | null |
Stroboscopic illumination measurements of GVs and theoretical models about shape fluctuation of GVs revealed that the archaeal lipid-based GVs showed the almost same value of bending elasticity modulus with synthetic lipid-based GVs.
| null | null |
life5021101_makarova
| 0 |
However, the comment of the reviewer is notable and we state in the conclusion of the manuscript (Lines 229–232) that at room temperature the thermophilicity is not necessarily related to the bending elasticity.
| null | null |
Round 1: and Author Response This manuscript described the measurement of bending elasticity of giant vesicles (GVs) prepared by archaea-derived lipids.
| null | null |
life5021101_makarova
| 0 |
Round 1: and Author Response This manuscript described the measurement of bending elasticity of giant vesicles (GVs) prepared by archaea-derived lipids.
| null | null |
The observed fluctuations of the membrane do not destabilize it.
| null | null |
life5021101_makarova
| 0 |
I also have questions and comments for acceptance.
| null | null |
The measurement itself is novel, but same methods and theories have been reported in the authors’ previous papers.
| null | null |
life5021101_makarova
| 0 |
However, the comment of the reviewer is notable and we state in the conclusion of the manuscript (Lines 229–232) that at room temperature the thermophilicity is not necessarily related to the bending elasticity.
| null | null |
The observed fluctuations of the membrane do not destabilize it.
| null | null |
life5021101_makarova
| 0 |
Second Round of Evaluation Round 2: and Author Response It is also not clear about the “thermally induced shape fluctuations”, such as the temperatures and incubation length and etc., especially this is a hyperthermophilic archaeal membrane vesicle, which would be expected to be more thermostable than those eukaryotic ones.
| null | null |
The observed fluctuations of the membrane do not destabilize it.
| null | null |
life5021101_perova
| 0 |
The observed fluctuations of the membrane do not destabilize it.
| null | null |
However, the comment of the reviewer is notable and we state in the conclusion of the manuscript (Lines 229–232) that at room temperature the thermophilicity is not necessarily related to the bending elasticity.
| null | null |
life5021101_perova
| 0 |
However, the comment of the reviewer is notable and we state in the conclusion of the manuscript (Lines 229–232) that at room temperature the thermophilicity is not necessarily related to the bending elasticity.
| null | null |
The observed fluctuations of the membrane do not destabilize it.
| null | null |
life5021101_perova
| 0 |
The observed fluctuations of the membrane do not destabilize it.
| null | null |
The observed fluctuations of the membrane do not destabilize it.
| null | null |
life5021101_perova
| 0 |
Second Round of Evaluation Round 2: and Author Response It is also not clear about the “thermally induced shape fluctuations”, such as the temperatures and incubation length and etc., especially this is a hyperthermophilic archaeal membrane vesicle, which would be expected to be more thermostable than those eukaryotic ones.
| null | null |
Second Round of Evaluation Round 2: and Author Response It is also not clear about the “thermally induced shape fluctuations”, such as the temperatures and incubation length and etc., especially this is a hyperthermophilic archaeal membrane vesicle, which would be expected to be more thermostable than those eukaryotic ones.
| null | null |
life5021101_perova
| 0 |
The observed fluctuations of the membrane do not destabilize it.
| null | null |
Second Round of Evaluation Round 2: and Author Response It is also not clear about the “thermally induced shape fluctuations”, such as the temperatures and incubation length and etc., especially this is a hyperthermophilic archaeal membrane vesicle, which would be expected to be more thermostable than those eukaryotic ones.
| null | null |
life5021101_perova
| 0 |
However, the comment of the reviewer is notable and we state in the conclusion of the manuscript (Lines 229–232) that at room temperature the thermophilicity is not necessarily related to the bending elasticity.
| null | null |
However, the comment of the reviewer is notable and we state in the conclusion of the manuscript (Lines 229–232) that at room temperature the thermophilicity is not necessarily related to the bending elasticity.
| null | null |
life5021101_perova
| 0 |
However, the comment of the reviewer is notable and we state in the conclusion of the manuscript (Lines 229–232) that at room temperature the thermophilicity is not necessarily related to the bending elasticity.
| null | null |
The observed fluctuations of the membrane do not destabilize it.
| null | null |
life5021101_perova
| 0 |
Second Round of Evaluation Round 2: and Author Response It is also not clear about the “thermally induced shape fluctuations”, such as the temperatures and incubation length and etc., especially this is a hyperthermophilic archaeal membrane vesicle, which would be expected to be more thermostable than those eukaryotic ones.
| null | null |
The observed fluctuations of the membrane do not destabilize it.
| null | null |
life5021101_perova
| 0 |
However, the comment of the reviewer is notable and we state in the conclusion of the manuscript (Lines 229–232) that at room temperature the thermophilicity is not necessarily related to the bending elasticity.
| null | null |
Second Round of Evaluation Round 2: and Author Response It is also not clear about the “thermally induced shape fluctuations”, such as the temperatures and incubation length and etc., especially this is a hyperthermophilic archaeal membrane vesicle, which would be expected to be more thermostable than those eukaryotic ones.
| null | null |
life5021101_perova
| 0 |
However, the comment of the reviewer is notable and we state in the conclusion of the manuscript (Lines 229–232) that at room temperature the thermophilicity is not necessarily related to the bending elasticity.
| null | null |
Second Round of Evaluation Round 2: and Author Response It is also not clear about the “thermally induced shape fluctuations”, such as the temperatures and incubation length and etc., especially this is a hyperthermophilic archaeal membrane vesicle, which would be expected to be more thermostable than those eukaryotic ones.
| null | null |
life5021101_perova
| 0 |
Second Round of Evaluation Round 2: and Author Response It is also not clear about the “thermally induced shape fluctuations”, such as the temperatures and incubation length and etc., especially this is a hyperthermophilic archaeal membrane vesicle, which would be expected to be more thermostable than those eukaryotic ones.
| null | null |
Second Round of Evaluation Round 2: and Author Response It is also not clear about the “thermally induced shape fluctuations”, such as the temperatures and incubation length and etc., especially this is a hyperthermophilic archaeal membrane vesicle, which would be expected to be more thermostable than those eukaryotic ones.
| null | null |
life5021101_perova
| 0 |
Second Round of Evaluation Round 2: and Author Response It is also not clear about the “thermally induced shape fluctuations”, such as the temperatures and incubation length and etc., especially this is a hyperthermophilic archaeal membrane vesicle, which would be expected to be more thermostable than those eukaryotic ones.
| null | null |
However, the comment of the reviewer is notable and we state in the conclusion of the manuscript (Lines 229–232) that at room temperature the thermophilicity is not necessarily related to the bending elasticity.
| null | null |
life5021101_perova
| 0 |
Second Round of Evaluation Round 2: and Author Response It is also not clear about the “thermally induced shape fluctuations”, such as the temperatures and incubation length and etc., especially this is a hyperthermophilic archaeal membrane vesicle, which would be expected to be more thermostable than those eukaryotic ones.
| null | null |
Second Round of Evaluation Round 2: and Author Response It is also not clear about the “thermally induced shape fluctuations”, such as the temperatures and incubation length and etc., especially this is a hyperthermophilic archaeal membrane vesicle, which would be expected to be more thermostable than those eukaryotic ones.
| null | null |
life5021101_perova
| 0 |
The observed fluctuations of the membrane do not destabilize it.
| null | null |
However, the comment of the reviewer is notable and we state in the conclusion of the manuscript (Lines 229–232) that at room temperature the thermophilicity is not necessarily related to the bending elasticity.
| null | null |
life5021101_perova
| 0 |
Second Round of Evaluation Round 2: and Author Response It is also not clear about the “thermally induced shape fluctuations”, such as the temperatures and incubation length and etc., especially this is a hyperthermophilic archaeal membrane vesicle, which would be expected to be more thermostable than those eukaryotic ones.
| null | null |
The observed fluctuations of the membrane do not destabilize it.
| null | null |
life5021101_perova
| 0 |
However, the comment of the reviewer is notable and we state in the conclusion of the manuscript (Lines 229–232) that at room temperature the thermophilicity is not necessarily related to the bending elasticity.
| null | null |
However, the comment of the reviewer is notable and we state in the conclusion of the manuscript (Lines 229–232) that at room temperature the thermophilicity is not necessarily related to the bending elasticity.
| null | null |
life5021101_perova
| 0 |
The observed fluctuations of the membrane do not destabilize it.
| null | null |
However, the comment of the reviewer is notable and we state in the conclusion of the manuscript (Lines 229–232) that at room temperature the thermophilicity is not necessarily related to the bending elasticity.
| null | null |
life5021101_perova
| 0 |
In summary, this paper is of good quality and of enormous value, and should be published.
| null | null |
This is a great paper to aim at such a direction.
| null | null |
life5021427_makarova
| 0 |
While not especially flashy or exciting, the authors tackle a serious problem, the reliability of annotations in publically available databases.
| null | null |
Importance: Archaea is/are the third domain of life, no more or less!
| null | null |
life5021427_makarova
| 0 |
While not especially flashy or exciting, the authors tackle a serious problem, the reliability of annotations in publically available databases.
| null | null |
The authors describe an alternative, manual curation strategy aimed at avoiding over-annotation.
| null | null |
life5021427_perova
| 0 |
The manuscript describes in detail the approach used by the HaloLex genome annotation system to obtain reliable, consistent annotations, based on “Gold Standard Proteins”.
| null | null |
The curation procedure is done, to my mind, very well; and I am confident that this will help the archaea community enormously.
| null | null |
life5021427_perova
| 0 |
The manuscript contains scientific material that can be published as a short communication.
| null | null |
Line 38: Deinococcus radiodurans should be written in italic.
| null | null |
life6020022_makarova
| 0 |
First of all it is necessary to change the title and at least remove “The testing”.
| null | null |
The manuscript contains scientific material that can be published as a short communication.
| null | null |
life6020022_makarova
| 0 |
Knowing the grain sizes and assuming a homogeneous distributrion of cells and grains or a measurement of the real distribution an assessment can be made about the shading effect of the grains with respect to UV radiation.
| null | null |
Line 78: Please explain in the text what is “a vacuum treatment control”.
| null | null |
life6020022_makarova
| 0 |
Line 38: Deinococcus radiodurans should be written in italic.
| null | null |
The authors should have estimated the maximal theoretically achievable effect and to plan the experiments accordingly.
| null | null |
life6020022_makarova
| 0 |
Minor comments (please see changes in text at the relevant lines)
| null | null |
Minor comments Lines 31-32: Bacillus subtilis and B.pumilus should be written in italic.
| null | null |
life6020022_makarova
| 0 |
Discussion How do you explain the difference between the results when using JSC Mars-1A analog and the other soils?
| null | null |
As the samples are placed in an ICP discharge, ions towards the samples are only accelerated by several tenth of eV.
| null | null |
life6020022_makarova
| 0 |
The exposure times varied from 1.5 to 45 min with different experiment Conditions/procedures without resulting in a significant inactivating effect on the bacterial cells.
| null | null |
Knowing the grain sizes and assuming a homogeneous distributrion of cells and grains or a measurement of the real distribution an assessment can be made about the shading effect of the grains with respect to UV radiation.
| null | null |
life6020022_makarova
| 0 |
The manuscript contains scientific material that can be published as a short communication.
| null | null |
Estimations of the average free length of path for the inactivating plasma components might have been helpful for experiment planning.
| null | null |
life6020022_makarova
| 0 |
The authors should have estimated the maximal theoretically achievable effect and to plan the experiments accordingly.
| null | null |
However, there are some remarks on the justification of objectives of the study and presentation of the results.
| null | null |
life6020022_makarova
| 0 |
However, there are some remarks on the justification of objectives of the study and presentation of the results.
| null | null |
As the samples are placed in an ICP discharge, ions towards the samples are only accelerated by several tenth of eV.
| null | null |
life6020022_makarova
| 0 |
Discussion How do you explain the difference between the results when using JSC Mars-1A analog and the other soils?
| null | null |
The manuscript contains scientific material that can be published as a short communication.
| null | null |
life6020022_makarova
| 0 |
The manuscript contains scientific material that can be published as a short communication.
| null | null |
The mineralogical composition is given in table, but not the grain size distribution.
| null | null |
life6020022_makarova
| 0 |
Estimations of the average free length of path for the inactivating plasma components might have been helpful for experiment planning.
| null | null |
With the knowledge of the spectral irradiance in the UV range from 100 to 400 nm the authors could have made a calculation to estimate the inactivation of D. radiodurans by the UV component of the plasma.
| null | null |
life6020022_makarova
| 0 |
In addition, I think, a control consisting of D. radiodurans cells before and after sterilization is missing to determine the role of the Martian Analog soils.
| null | null |
For the argon-oxygen gas mixture it is stated that ‘high amounts of reactive oxygen species are formed in the plasma’ again without any qualitative and quantitative description.
| null | null |
life6020022_makarova
| 0 |
Line 78: Please explain in the text what is “a vacuum treatment control”.
| null | null |
The other potentially inactivating components of the plasma are not specified in the manuscript.
| null | null |
life6020022_perova
| 0 |
Line 38: Deinococcus radiodurans should be written in italic.
| null | null |
If I understood correctly, you used only one medium.
| null | null |
life6020022_perova
| 0 |
The mineralogical composition is given in table, but not the grain size distribution.
| null | null |
However, there are some remarks on the justification of objectives of the study and presentation of the results.
| null | null |
life6020022_perova
| 0 |
The authors should have estimated the maximal theoretically achievable effect and to plan the experiments accordingly.
| null | null |
Reply: The names are written in italics now, the mistake must have happened during the editing of the text.
| null | null |
life6020022_perova
| 0 |
Line 78: Please explain in the text what is “a vacuum treatment control”.
| null | null |
The authors should have estimated the maximal theoretically achievable effect and to plan the experiments accordingly.
| null | null |
life6020022_perova
| 0 |
Line 78: Please explain in the text what is “a vacuum treatment control”.
| null | null |
Line 78: Please explain in the text what is “a vacuum treatment control”.
| null | null |
life6020022_perova
| 0 |
The manuscript contains scientific material that can be published as a short communication.
| null | null |
However, there are some remarks on the justification of objectives of the study and presentation of the results.
| null | null |
life6020022_perova
| 0 |
For the argon-oxygen gas mixture it is stated that ‘high amounts of reactive oxygen species are formed in the plasma’ again without any qualitative and quantitative description.
| null | null |
It is only stated that the argon-nitrogen mixture ‘mainly emits high amounts of radiation in the wavelength region from 100 nm to 400 nm’ without specifying what high fluences at the sample site are.
| null | null |
life6020022_perova
| 0 |
It is only stated that the argon-nitrogen mixture ‘mainly emits high amounts of radiation in the wavelength region from 100 nm to 400 nm’ without specifying what high fluences at the sample site are.
| null | null |
First of all it is necessary to change the title and at least remove “The testing”.
| null | null |
life6020022_perova
| 0 |
Discussion How do you explain the difference between the results when using JSC Mars-1A analog and the other soils?
| null | null |
While writing D. radiodurans R1 has always necessary to note that this is type strain R1T.
| null | null |
life6020022_perova
| 0 |
First of all it is necessary to change the title and at least remove “The testing”.
| null | null |
In the manuscript ‘Testing the Effects of Low-Temperature Plasma-Sterilization on Mars Analog Soil Samples Intermixed with Deinococcus radiodurans’ by Schirmack et al., the results of the application of low-temperature plasma on Mars regolth simulant mixed with cells of the bacterium D. radiodurans were reported.
| null | null |
life6020022_perova
| 0 |
The authors’ like to thank the reviewer for the helpful comments and suggested corrections to improve the manuscript.
| null | null |
The authors should have estimated the maximal theoretically achievable effect and to plan the experiments accordingly.
| null | null |
life6020022_perova
| 0 |
Also, as far as this reviewer is aware, there are no known general (= nonspecific) RNA replicase ribozymes consisting of only 100 nucleotides.
| null | null |
Dear academic editors I response to all the comments by reviewer 1.
| null | null |
life6030029_makarova
| 0 |
I agree that the question of the relationship between RNA World and the environment is important, but there is nothing in the main part of this article that addresses this question.
| null | null |
It is also fairly rambling: for example, lines 95-106 could be removed, Answer:
| null | null |
life6030029_makarova
| 0 |
Can we not have an RNA polymerase that uses other copies of itself as a template?
| null | null |
The input and output would not necessarily need to be controlled by another gene.
| null | null |
life6030029_makarova
| 0 |
Once again in Fig 8 I think that the cycle is over-emphasized and the throughput is not included.
| null | null |
Reviewer 1 line 38 - The linking of 'other molecules' to 'phenotype' here is a little odd.
| null | null |
life6030029_makarova
| 0 |
The connection between the reactions catalyzed by the ribozymes and the concepts of CCSI and CMIO is not very clear at this point.
| null | null |
It is also fairly rambling: for example, lines 95-106 could be removed, Answer:
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life6030029_makarova
| 0 |
This section seems to mix up two important questions.
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The input and output would not necessarily need to be controlled by another gene.
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life6030029_makarova
| 0 |
More fundamentally perhaps, the distinction that is made between CCSI and CMIO is, as the author himself notes, remarkably similar to that between genotype and phenotype, and information and function, and even replication and metabolism.
| null | null |
Fig 5 - drawing parallels between systems of different levels of complexity (from prokaryotes up to civilizations) is interesting, but it is a bit distracting at this point.
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life6030029_makarova
| 0 |
So I think this idea needs to be explained and justified in a biological context.
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The abstract promised to talk about RNA World and the origin of life.
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life6030029_makarova
| 0 |
lines 93-106 - I mostly agree with this paragraph but it is not said very clearly.
| null | null |
Once again in Fig 8 I think that the cycle is over-emphasized and the throughput is not included.
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life6030029_makarova
| 0 |
Another interesting idea that is also raised is the possible importance of RNA solubility at high temperatures, though it does seem that the author is battling nobly (if possibly in vain) to reconcile the RNA world hypothesis with a hydrothermal vent origin of life scenario.
| null | null |
Also, as far as this reviewer is aware, there are no known general (= nonspecific) RNA replicase ribozymes consisting of only 100 nucleotides.
| null | null |
life6030029_makarova
| 0 |
The input and output would not necessarily need to be controlled by another gene.
| null | null |
Fig 7 - Viewing a metabolism as a cycle seems to be only half the story.
| null | null |
life6030029_makarova
| 0 |
One example obvious to me is the reference to one of my papers [ref.
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There are many quantitative traits affected by many genes.
| null | null |
life6030029_makarova
| 0 |
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