text
stringlengths 10
10.1k
| source
stringclasses 1
value |
|---|---|
we have constructed an adenovirus type ad recombinant virus in which the early region b eb of the nononcogenic ad is replaced by the eb region of the highly oncogenic ad analysis of cells lytically INF with the recombinant virus showed that both the ad ea genes and the ad eb genes are faithfully expressed the recombinant virus replicates efficiently in human embryonic kidney cells and in hela cells indicating that the ad eb region can fully replace the ad eb region in lytic infection inoculation of the adad hybrid virus into P0 hamsters did not result in development of tumors this shows that the eb region of ad previously shown to be responsible for the high oncogenic potential of adtransformed cells in nude mice is not capable of converting the nononcogenic ad into an oncogenic virus
|
MEDAL
|
we hypothesized that orthostatic intolerance oi is associated with gastric dysrhythmias nausea and ABD pain which improves using fludrocortisone to treat oi
|
MEDAL
|
urea transporter uta the L1 urea transporter of the thin descending limb of the loop of henle in short loop nephrons has been implicated in urea recycling in the medulla thereby producing concentrated urine to investigate the physiological role of uta in vivo we generated utaselective KO by deleting the uta promoter western analysis immunohistochemistry and quantitative reverse transcriptionpcr were used to confirm the specific deletion of uta with preservation of other uta transporters compared to WT differences in the urine outputs of uta mice consuming a normal protein diet protein were not observed under normal conditions or with dehydration likewise impairment of urea accumulation in the inner medulla of uta mice was not observed on a lowprotein diet protein however significantly reduced maximal urine osmolality was observed in dehydrated uta mice compared to wildtype littermates mosmol versus mosmol respectively a significant reduction in urea accumulation in the inner medulla was also observed in uta mice however differences in na and cl accumulation were not observed thus uta is important for maintaining a high concentration of urea in the IM when urea supply to the kidney is limited
|
MEDAL
|
the purposes of this pilot study were as follows to determine the percentage and frequency of dental hygiene graduates who were providing tobacco cessation counseling tcc activities after receiving tcc training during their formative years of education to identify the graduates stages of change using the transtheoretical model regarding tcc and to identify AF influencing the delivery of tcc a convenience sample of fiftyone minnesota state university mankato msu dental hygiene graduates were surveyed in this study wilcoxen signed rank tests were used to analyze the relationship between counseling actions and dental hygienists stage of change in four tcc activities asking advising assisting and arranging followup pros and cons affecting the delivery of tcc were also examined findings indicate that dental hygienists who received tcc education during their training do not provide cessation counseling to a high percentage of their patients even though they believe in the value of such activity however dental hygienists are more likely to provide tcc to patients with periodontal disease than other dental patients
|
MEDAL
|
bathing HPC slices in the potassium channel blocker tetraethylammonium tea while stimulating the schaffer collaterals at a low frequency induces cadependent nmethyldaspartate nmda receptorindependent LT potentiation of synaptic transmission ltpk in ca neurons we have combined ratio imaging of fura and magfura in hippocampal ca SN with intracellular and field recordings to evaluate postsynaptic ca changes that occur in the induction of ltpk test stimuli were applied at hz to SR in the presence of the nmda receptor antagonists dlaminophosphonovaleric acid microm or mk microm during tea exposure mm min cells fired prolonged action potentials both spontaneously and in response to test stimuli resulting in transient micromolar ca accumulations in both somata and dendrites the initial epsp slope measured min after tea washout was potentiated to approximately of control the ca channel blocker nimodipine microm greatly reduced ca transients in both magnitude and duration and prevented ltpk induction pretreatment of slices with EDC that block metabotropic glutamate receptor mglurstimulated phosphoinositide hydrolysis laminophosphonopropionic acid lap microm or laspartatebetahydroxamate microm as well as protein kinase c pkc inhibitors sphingosine microm ro microm or calphostin c microm also blocked ltpk ca transients were unaffected by lap or ro these findings suggest that ca influx through Kv channels and coactivation of pkc by mglurs are both necessary for induction of ltpk activation of mglurs must also occur in nmda receptordependent induction paradigms but is possibly of lesser importance owing to the much greater gating of ca directly into the dendritic spines
|
MEDAL
|
the effects of insulin on fetal growth rates were investigated by measuring crownrump length crl chronically in sheep fetuses before and after pancreatectomy sham operation or pancreatectomy accompanied by insulinreplacement treatment pancreatectomy of the fetus produced hypoinsulinaemia and reduced the daily and total increments in crl by over the last days of gestation the growth rate fell immediately T3 pancreatectomy and remained low throughout the rest of gestation in contrast to the shamoperated fetus no prepartum decline in the growth rate was observed in the pancreatectomized animal the body weight limb lengths and actual crl at delivery were also significantly less T3 pancreatectomy than in the shamoperated animals when fetal insulin C2 were maintained T3 pancreatectomy by exogenous insulin infusion the daily and total increments in crl and the body size at delivery were restored to their normal values raising insulin levels above normal in the pancreatectomized fetus did not lead to additional fetal growth when the data from all the fetuses were combined irrespective of treatment there were significant positive correlations between the plasma insulin concentration in utero and the mean daily increment in crl T3 treatment and between the former value and the body weight at delivery these observations demonstrate that insulin is a L1 determinant of the growth rate in utero and that it is required throughout late gestation for the normal growth and development of the sheep fetus they also indicate that insulin deficiency is probably the primary cause of the growth retardation obsee human hepatocytic antigens in the selfperpetuation of the inflammatory processes was investigated in two CG of patients with chronic AS liver disease cald in the patients of the first group with chronic AS SH and with active cirrhosis their serum was put in contact with a suspension of hepatocytes in a culture of liver from a monthold human embryo in presence of rabbit complement the hepatocytic lysis was evaluated by the test of microcytotoxicity mct considered positive at values above ten healthy subjects and patients with atrophic gastritis were used as controls in other patients with cald nd group the authors performed the test of inhibition of the macrophage migration imm and that of lymphoblastic transformation lt in presence of an autologous CL antigen obtained by liver biotic puncture in the G1 the mct test was positive in of the patients in rho with a particular biomorphologic aggressivity and marked immune disturbances while in controls it was negative in of the cases in the second group the extract of autologous HCs induced a marked inhibition of the macrophage migration and a significantly increased lymphoblastic transformation it is concluded to the existence in the patients with cald of cytotoxic hepatocytic antibodies as well as to a stimulation of the macrophagiclymphocytic CF in presence of the autologous CL antigen the participation af an autoimmune mechanism in the perpetuation of the hepatocytic lesions in such patients is also suggested
|
MEDAL
|
maternal cervical incompetence in pregnancy cip showed an association with a higher rate of preterm births the objective of this T0 was to determine the prevalence of cip in hungarian pregnant women to determine the rate of PTB and to check the preventive tau of PT births due to cip by therapeutic cerclage or BR alone
|
MEDAL
|
we describe the management of a young boy with mild haemophilia a and a massive iliac pseudotumour with a multi modality approach involving factor replacement radiation therapy embolization and surgery the patient was initially treated with recombinant factor viii and radiation therapy because of inadequate response and worsening of bony erosion the patient had a preoperative embolization followed by surgical excision the surgical procedure was associated with minimal BL and the patient had a relatively smooth postoperative course with no physical morbidity this case illustrates successful aggressive management of a large proximally located pelvic pseudotumour which resulted in an excellent outcome despite the need for a normally morbid operation
|
MEDAL
|
parabens alkyl esters of phydroxybenzoic acid are widely used as preservatives in food cosmetics and pharmaceutical products however weak estrogenicity of some parabens has been reported in several studies which provided the impetus for this work here a simple and efficient analytical method for quantifying parabens in cancer tissues has been developed this technique involves the simultaneous use of microwaveassisted solvent extraction mase and microsolid phase extraction μspe in tandem with high performance liquid chromatography hplcuv analysis for the determination of parabens the pollutants studied included four parabens methyl ethyl propyl and butyl parabens optimization of the exp parameters for mase and μspe was performed good relative standard deviation rsd ranged from to and high enrichment factors were obtained coefficients of determination r up to were obtained across a concentration range of ngg the method detection limits for parabens ranged from to ngg the procedure was initially tested on prawn samples to demonstrate its feasibility on a complex biological matrix preliminary studies on human OC oc tissues showed presence of parabens higher C2 of parabens were detected in malignant ovarian RT tissues compared to benign tumor tissue samples
|
MEDAL
|
glucokinase plays a key role in the metabolism of glucose by pancreatic βcells in this T0 the effects of the glucokinase activator gka on cell volume and electrical activity in rat βcells were examined one micro molar gka caused an increase in βcell volume in the presence of mm glucose gka also caused a depolarisation of βcell membrane potential and increased electrical activity these changes were associated with the activation of inward wholecell currents and were attenuated by the anion channel inhibitor nitrophenylpropylamino benzoic acid in single channel experiments the open probability of volumeregulated anion channels vrac was increased from ± to ± n by the gka the data suggest that a gkaevoked increase in gl metabolism causes an increase in βcell volume this in turn activates vrac leading to a depolarisation of the cell membrane potential
|
MEDAL
|
succinylacetone an abnormal metabolite of the IMT metabolic pathway is produced in patients with hereditary tyrosinemia because of a genetic deficiency of fumarylacetoacetase this metabolite greatly inhibits the activity of ala dehydratase and accounts for the elevated excretion of ala in urine in this disease we have studied the effects of SA on heme synthesis in cultured avian hepatocytes and on the activity of purified ala dehydratase from normal human erythrocytes and from mouse CL succinylacetone markedly inhibited ala dehydratase in a competitive manner the compound also decreased cellular heme and cytochrome p content and greatly potentiated the drug and steroid inhe G1 PTT time aptt was given during pregnancy as prophylaxis or therapeutic treatment during delivery and abortion the at level was brought to normal by infusion of at concentrate and the heparin was reduced or withdrawn four pregnancies were uncomplicated with regard to te and resulted in HC five pregnancies were terminated by induced or SAB woman had te during heparin prophylaxis and women had te before the prophylaxis was started of the latter suffered from a new te during continued heparing treatment insufficient prolongation of aptt was registered at the time of te in both women with te during heparin treatment
|
MEDAL
|
organotypic cultures of cerebral mantle offer a useful method for in vitro investigation of mechanisms involved in the migration of neuronal precursors to the neocortex studies reported todate have indicated that while SN continue to migrate in CBF explant cultures for a few days they eventually stop migrating and fail to reach their preprogrammed destinations and thus distinct layers fail to develop within the CP hypothesizing that this migrational arrest stemmed in part from severe distortion and flattening of explants in the rollertube SC method used in previous studies by others we studied neocortical neuronal migration using a recently developed threedimensional explant SC system explants microm thick were derived from e fetal mouse cerebrum h following administration of bromodeoxyuridine brdu encased in collagen placed on porous CM and alternately exposed to medium and air on a rocking platform cultures were maintained for and days then analyzed for the location of brdulabeled cells newborn mice labeled at e and analyzed at p and p served as controls in the explants labeled cells continued to migrate into the CP until days in culture at which point the VVI zone had disappeared and two distinct cortical cell layers sometimes developed brdulabeled cells were present throughout the cortical plate particularly the mid portion in p controls five distinct cortical layers were present with labeled cells mostly in the outer half layers ii iii thus preservation of threedimensional tissue integrity in SC was accompanied by a modest improvement in migration of SN to the neocortex in comparison with previous reports the fact that normal SL formation failed to develop in thick explants suggests that factors other than tissue distortion contribute to this outcome
|
MEDAL
|
the title compound chno is a functionalized triazoloquinazoline with a substituted benzyloxy group attached at the position of a triazole spacer the triazoloquinazoline fusedring system is approximately planar rms deviation Ã¥ while the benzyl substituent is perpendicular to the ring system making a dihedral angle of boson and the photon zγ couplings vanish at tree level and are heavily suppressed at higher orders hence any evidence of couplings indicates new physics measurements are performed using data corresponding to an integrated luminosity of fb in the zâνν¯ decay channel and fb in the zâll lμ e decay channels the combination of these measurements provides the most stringent limits to date on zγ trilinear gauge couplings using an energy scale of λ tev to allow for a direct comparison with previous measurements we find limits on the cpconserving parameters that describe zγ couplings to be hγz and hγz these results are consistent with standard model predictions
|
MEDAL
|
coordination of mental CHS has often been a challenge from the users point of view resulting gaps in care can lead to increased use of crisis services more frequent hospitalization and impaired quality of life this study examined an innovative approach to building an individuals selfcare capacities by testing the use of a wellness MI an adaptation of a clientheld medical recordcrisis plan in booklet form fifty individuals using community mental health services were recruited using a pretestposttest design participants completed measures of empowerment continuity of care quality of life and satisfaction with services at the start of and again following months of using the booklet the booklet referred to as the wellness planner included components that have been empirically demonstrated as effective such as crisis planning goal setting and resource planning for the individuals who completed the study statistically significant increases were seen in empowerment continuity of care and satisfaction with services T3 months of using the wellness planner qualitative data further demonstrated positive acceptance of the booklet by the users findings of the T0 suggest that the use of such a booklet could not only have a positive impact on the REC of individuals but could also have utility within the mental health system since the completion of this T0 the winnipeg RHA has adopted the booklet for use within all its AD MH programs future research will focus on the impact of the booklet on systemwide indicators such as service utilization and readmission rates
|
MEDAL
|
we recently developed a genetic transneuronal tracing approach that allows for the T0 of circuits that are altered by nerve injury we generated transgenic zwx mice in which expression of a transneuronal tracer wheat germ agglutinin wga is induced in primary sensory neurons but only after transection of their peripheral axon by following the transneuronal transport of the tracer into the CE NS cns we can label the circuits that are engaged by the wgaexpressing damaged SN here we used the zwx mouse line to analyze dorsal root ganglia drg for intraganglionic connections between injured SNs and their neighboring IN neurons because NPY-LI npy expression is strongly induced in drg neurons after IP axotomy we crossed the zwx mouse line with a mouse that expresses cre recombinase under the influence of the npy promoter as expected sciatic nerve transection triggered wga expression in npypositive drg neurons most of which are of large diameter as expected double labeling for atf a marker of cell HB with damaged axons showed that the tracer predominated in injured ie axotomized SN however we also found the wga tracer in drg cell HB of uninjured SNs importantly in the absence of nerve injury there was no intraganglionic transfer of wga our results demonstrate that intraganglionic celltocell communication via transfer of C1 molecules occurs between the cell bodies of injured and neighboring noninjured primary afferent SN
|
MEDAL
|
using TD echocardiography we evaluated left VVI longaxis CF in MS patients of whom were in SR and of whom were in chronic atrial fibrillation there were healthy control subjects conventional echocardiography was used to evaluate left VVI diameters left atrial diameters left ventricular ejection fractions and mitral valve areas TD echocardiography was used to evaluate isovolumetric contraction time ict isovolumetric EC50 time irt ET et and peak systolic myocardial E/A the myocardial performance index was calculated with the formula ictirtet mean and segmental left VVI irt and ict values were significantly longer in MS patients than in control subjects but were similar in the sinus rhythm and atrial fibrillation subgroups ejection times were significantly lower in the MS with atrial fibrillation group than in the MS with sinus rhythm and control groups p but were similar when the sinus rhythm group was compared with the control group p myocardial performance index values were higher in mitral stenosis patients significantly so in those with atrial fibrillation when compared with the control group p systolic velocity values were significantly lower in mitral stenosis patients more markedly so in those with atrial fibrillation than in the control group p we conclude that patients with pure rheumatic mitral stenosis most particularly when in combination with atrial fibrillation have significantly impaired left ventricular longaxis function as evaluated by tissue doppler echocardiography although global systolic CF may be normal
|
MEDAL
|
the aim was to investigate gastric myoelectrical activity gma and vagal activity in response to stress the study was performed in HS in three sessions control EC50 and AS the control session was composed of min recordings before and min recordings after a test meal the protocol of two other sessions was similar except that the fasting recording was extended to min and the subjects were continuously watching a horror movie stress or guided meditation tape EC50 T3 the min baseline gma was recorded using EGG and HRV hrv was derived from the electrocardiogram meal resulted in a postprandial increase in the dominant frequency cpm vs cpm p dominant SP db vs db p and percentage of normal SWs vs p similar responses were found in the EC50 session stress inhibited all these normal PP response and reduced the regularity of gastric slow waves vs p in addition SA of the hrv demonstrated an inhibition of postprandial vagal activity and an increase of postprandial sympathetic activity with stress stress has an inhibitory effect on postprandial gma and this may involve both vagal and sympathetic pathway
|
MEDAL
|
recurrent and M1 medullary thyroid carcinoma mtc remains difficult to treat due to its limited responsiveness to chemotherapy radiotherapy and imaging to investigate an alternative therapeutic approach we examined the feasibility of targeting genedirected enzymeprodrug therapy delivered by adenoviral vectors to mtc we previously described a modified human calcitonin ctct generelated peptide promoter that produced increased expression while maintaining specificity for mtc cells in this T0 we introduced an additional level of specificity by using cellspecific splicing and examined whether the selectivity of the genedirected enzymeprodrug therapy for mtc was enhanced when both the promoter and splicing features were combined in a single transcription unit two replicationdefective adenoviruses were cons imaging of the head and neck regions were evaluated and the impact that tumour chronology the tumour site and the various treatment modalities have on the survival were assessed fortytwo per cent of patients developed a metachronous second malignancy more than five years T3 diagnosis of the index tumour the accuracy of colourduplex US in PCD of second primaries in the head and neck was per cent first and second primary tumours located in the larynx were observed to have the highest fiveyear survival rate patients who developed metachronous tumours had a fiveyear survival rate of per cent for the index tumours and a per cent fiveyear survival rate with the occurrence of a second neoplasm with synchronous tumours a mean survival time of months and a fiveyear survival rate of per cent was found p where clinically appropriate an aggressive treatment strategy was employed and yielded the most favourable results with a fiveyear survival rate of per cent and per cent for index tumours and second primary malignancies respectively since more than per cent of the metachronous second primaries in patients with a history of head and neck malignancy occur beyond the fiveyear followup period an D2 protocol with individually adjusted close monitoring of highrisk patients seems AGA colourduplex sonography is a valuable screening investigation for the early PCD of second primary tumours the treatment of a second primary is often less successful than for the same malignancy occurring primarily the prognosis of synchronous tumours is significantly lower when compared to malignancies of a metachronous nature despite some encouraging individual results only the early implementation of aggressive treatment methods for second primaries is successful in terms of survival
|
MEDAL
|
for the purpose of identifying genes that affect BV/TV we previously identified two inbred mouse strains cblj and C3 with C1 differences in femoral BD and medullary cavity volume the lower density and larger medullary cavity volume in cblj mice could result from either decreased formation or increased resorption or both we recently reported evidence suggesting that bone formation was increased in vivo and that osteoblast progenitor cells are more numerous in the bone marrow of chhej compared with cblj mice in the present study we determined whether osteoclast numbers in vivo and osteoclast formation from bone marrow cells in vitro might also differ between the two mouse strains we have found that the number of osteoclasts on bone surfaces of distal humerus secondary spongiosa was fold higher in weekold cblj mice than in C3 mice of the same age p bone marrow cells of cblj mice cocultured with swisswebster mouse osteoblasts consistently produced more osteoclasts than did chhej bone marrow cells at all ages tested from weeks of age p osteoclast formation was also greater from SP cells of weekold cblj mice than chhej mice the CSD of nuclei per OCL and the dihydroxyvitamin d dose dependence of OCL production from bone marrow cells were similar osteoclasts that developed from both cblj and C3 marrow cells formed pits in dentin slices cultures from cblj marrow cells formed fold more pits than cultures from C3 marrow cells p we compared the abilities of cblj and C3 osteoblasts to support OCL formation when bone marrow cells from either cblj or C3 mice were cocultured with osteoblasts from either cblj or C3 newborn calvaria the CS from which OBs were derived did not affect the number of osteoclasts formed from marrow cells of either strain together these observations suggest that genes affecting the bone marrow osteoclast precursor population may contribute to the relative differences in BD that occur between C3 and B6 mouse strains
|
MEDAL
|
experiments were conducted to determine whether muscarinic receptors within the PPN ppn and VTA vta are involved in regulating ethanol drinking SMB in the alcoholpreferring p L1 of rats female p rats were given limited access hday to vv ethanol and g ml CS solutions food was available ad libitum cholinergic agents were microinjected unilaterally into the ppn or vta immediately prior to ethanol access intrappn carbachol microg microl which can inhibit cholinergic neuronal activity within the ppn decreased ethanol decrease at the highest dose p and CS decrease at the highest dose p NI in a dosedependent manner within the first min intrappn scopolamine microg microl which can stimulate ACh neuronal activity within the ppn decreased ethanol NI in a dosedependent manner within the first min decrease at the highest dose p without reducing saccharin intake intravta methylscopolamine microg microl a muscarinic antagonist significantly p reduced ethanol decrease at the highest dose and saccharin decrease at the highest dose intakes during the h access period intravta carbachol a cholinergic agonist and microg microl decreased ethanol consumption in a dosedependent manner within the first min decrease at the highest dose without reducing CS NI overall these results support an involvement of the cholinergic ppnvta system in regulating alcohol drinking and general consummatory behaviors of the p L1 of rats
|
MEDAL
|
biophysical cues such as substrate modulus have been shown to influence a variety of cell behaviors we have determined the EM of the anterior basement membrane and DM of the human cornea with atomic force microscopy afm a spherical probe was used with a radius approximating that of a typical cell focal adhesion values obtained for the elastic modulus of the anterior basement membrane range from to kpa with a mean of kpa the elastic modulus of descemets membrane was found to be slightly higher than those observed for the anterior basement membrane with a mean of kpa and a range of kpa the topography of DM has been shown to be similar to that of the anterior basement but with smaller pore sizes resulting in a more tightly packed structure this structural difference may account for the observed modulus differences the determination of these values will allow for the design of a better MM of the cellular environment as well as aid in the design and fabrication of artificial corneas
|
MEDAL
|
ascitic fluid ph and arterialascitic fluid ph gradient were compared to ascitic fluid PMN in patients with cirrhotic ascites and in with malignant ascites to assess their role as diagnostic tests for spontaneous bacterial peritonitis and to clarify the relationship between ascitic fluid ph and lactate ascitic fluid ph was significantly lower ph in SBP n and probable spontaneous bacterial peritonitis n than in sterile ascites ph n since blood ph C2 were not different in the presence of infection arterialascitic fluid ph gradient was significantly higher in spontaneous bacterial peritonitis and probable spontaneous bacterial peritonitis than in sterile ascites vs the close correlations between arterialascitic ph gradient and lactate r p less than lactate and bicarbonate gradient r p and arterialascitic ph gradient and pco gradient r p less than suggest that the low ascitic fluid ph may be due to an increase in lactate and co patients with escherichia coliinduced SBP had significantly lower ascitic fluid ph and higher lactate than those with SBP by other organisms values of ascitic fluid ph lactate and arterialascitic fluid ph gradient in malignant ascites were similar to those of SBP and probable SBP cutoff points selected by receiver operating characteristic curves analysis of per mm for polymorphonuclear cells and of for arterialascitic fluid ph gradient allow high positive and negative predictive values for spontaneous bacterial peritonitisabstract truncated at words
|
MEDAL
|
polylactidecotrimethylene carbonates were prepared by the lipasecatalyzed ringopening copolymerization of lactide and TMC having carbonate content from to their thermal properties and enzymatic degradability were measured the ll dd and dllactides were copolymerized with trimethylene carbonate by porcine pancreatic lipase to produce random copolymers having molecular weights of up to the glass transition temperature tg of the copolymer was dependent on the carbonate content and the tg values linearly decreased from degrees c polylactide to degrees c polytrimethylene carbonate among the lipases tested the porcine pancreatic lipase and PK show basal mitochondrial transcription apparatus of saccharomyces cerevisiae consists of the core enzyme for mitochondrial rna polymerase and the specificity factor the core enzyme is homologous to those of bacteriophages t t and sp whereas the specificity factor shows similarities with bacterial sigma AF recently it was shown that the bacteriophagetype core enzyme is widespread among the eukaryotic lineage and a common picture for the mitochondrial transcription apparatus in eukaryotic cells is now emerging in contrast to the situation for the core enzyme the gene for the specificity factor has only been identified from s cerevisiae and more recently from two other yeast species as the specificity factor is the key component for initiation of transcription at the mitochondrial promoter we wanted to study in more detail gene expression regulation and the CF of the promoter of the nuclear mtf gene for this purpose the messenger rna level for scmtf was investigated under a C1 number of different growth conditions and thereby exhibited a very low but regulated and carbon sourcedependent expression deletion experiments identify the minimal promoter for functional complementation in yeast to evaluate the functional conservation of the promoter elements the homologous mtf gene from the closely related yeast saccharomyces douglasii was isolated and tested in heterologous complementation experiments in spite of a highly conserved protein CS these studies demonstrate that at lowcopy number sdmtf is not able to substitute for scmtf in s cerevisiae promoter exchange experiments with mtf from s cerevisiae and s douglasii demonstrate that differences in gene expression are responsible for the failure in rat complementation this finding prompted us to compare the promoter regions of mtf from four different yeast species for this purpose the sequences of the regions from s douglasii s kluyveri and kluyveromyces lactis were determined a comparison of these sequences identifies significant differences and rapid changes in the intergenic regions even between closely related yeast species
|
MEDAL
|
previously we reported on a nonapeptide binding peptide derived by reading the complementary dna strand of the bovine arginine vasopressin avp gene in the direction that specifically blocks the avp helper signal for gammainterferon ifn gamma production by helper celldepleted mouse spleen cultures bovine avpbinding peptide however did not block avp activity we report here on the relative abilities of and avpbinding MMP derived from the bovine and rat avp genes to block the avp helper signal for ifn gamma production the sequences of the bovine and rat avpbinding MMP differ by two amino acids whereas the avpbinding MMP derived from both genes are the same in contrast to the lack of blocking activity of the bovine avpbinding peptide the rat avpbinding peptide was almost as effective as the avpbinding peptide in blocking avp CF no effect was seen with a CAA control peptide consisting of a scrambled avpbinding peptide sequence further polyclonal antirat avpbinding peptide antibodies blocked avp activity whereas polyclonal antibovine avpbinding peptide antibodies had no significant effect polyclonal antibodies generated against the avpbinding peptide also blocked avp activity these antibodies possibly blocked avp function by interaction with the lymphocyte avp receptor since avp specifically displaced IB of the antibodiesabstract truncated at words
|
MEDAL
|
GPP synthase catalyzes the condensation of isopentenyl diphosphate with dimethylallyl diphosphate to give a c compound geranyl diphosphate which is a precursor of all monoterpenoids however the gene has not been isolated from any organisms to examine the possibility that geranyl diphosphate synthase has evolved from a common ancestor of the prenyltransferase family and to predict the active site structure we tried to convert bacillus stearothermophilus FPP synthase to GPP synthase according to our previous findings several mutated farnesyl diphosphate synthases that have single CAA substitutions before the first aspartaterich motif were constructed a mutated enzyme that has the replacement of serine by phenylalanine at the fourth position before the motif exclusively produced geranyl diphosphate when dimethylallyl diphosphate was used as the primer and hardly accepted GPP as a primer indicating that this mutation causes the conversion to GPP synthase this result supports the idea that the product specificities of all members of the eprenyltransferase family are mainly defined by a few structural features the amino acids at the fourth position and the fifth position before the first aspartaterich motif and the insertion of two amino acids in the motif this suggests that natural GPP synthases might have an AS site structure similar to that of the mutated enzyme
|
MEDAL
|
between july and october patients male female aged from to years mean years with malignant BB gliomas underwent surgical procedures stereotactic biopsy in and resection in patients there were grade iv astrocytomas grade iv mixed oligoastrocytomas grade iii astrocytomas grade iii mixed oligoastrocytomas and malignant oligodendrogliomas overall median survival time was weeks for grade iv gliomas weeks for WHO gliomas and weeks for malignant oligodendrogliomas multivariate analysis in newly diagnosed cases revealed that the variables most strongly correlated with survival time were tumor grade patient age seizures as a first symptom a KPS score of less than tumor resection and a radiation therapy dose greater than gy the proportions of patients receiving tumor resection versus biopsy in each of these prognosis factor groups were similar since most of the patients with midline and brainstem PT were treated with biopsy alone these were excluded considering newly diagnosed patients with cortical and subcortical PT grade iv glioma patients undergoing resection of the contrastenhancing mass as evidenced on computerized tomography and magnetic resonance imaging and postoperative EBRT lived longer than those undergoing biopsy only and radiation therapy median survival time weeks and weeks respectively smirnov test p however survival in patients with resected WHO gliomas was no better than in those with biopsied WHO lesions p the authors conclude that in selected grade iv gliomas resection of the contrastenhancing mass followed by radiation therapy is associated with longer survival times than radiation therapy T3 biopsy alone
|
MEDAL
|
the glyoxylate shunt enzymes isocitrate lyase and malate synthase were present at high levels in mycelium grown on acetate as sole source of carbon compared with mycelium grown on sucrose medium the glyoxylate shunt activities were also elevated in mycelium grown on glu or casamino acids as sole source of carbon and in amino acidrequiring auxotrophic mutants grown in sucrose medium containing limiting amounts of their required CAA under conditions of enhanced catabolite repression in mutants grown in sucrose medium but starved of krebs cycle intermediates isocitrate lyase and malate synthase C2 were derepressed compared with the levels in wild type grown on sucrose medium this derepression did not occur in related mutants in which krebs cycle intermediates were limiting growth but catabolite repression was not enhanced no krebs cycle intermediate tested produced an efficient repression of ICL activity in acetate medium of the two forms of isocitrate lyase in neurospora isocitrate lyase constituted over of the ICL activity in acetategrown wild type and also in each of the cases already outlined in which the glyoxylate shunt activities were elevated on SU medium on the basis of these results it is concluded that the synthesis of isocitrate lyase and malate synthase in neurospora is regulated by a glycolytic intermediate or derivative our data suggest that isocitrate lyase and ICL are the products of different structural genes the metabolic roles of the two forms of isocitrate lyase and of the glyoxylate cycle are discussed on the basis of their metabolic control and intracellular localization
|
MEDAL
|
to compare the accuracy of rchads chads and chadsvasc scores vs the society of thoracic surgeons sts score as predictors of morbidity and mortality T3 cardiovascular surgery
|
MEDAL
|
the rhorhokinase pathway plays an important role in many cardiovascular diseases such as hypertension atherosclerosis HF and myocardial infarction although previous studies have shown that ROK inhibitors reduce ischemiareperfusion ir injury and cytokine production the role of ROK in WBCs during ir injury is not well understood mice were subjected to min ischemia and reperfusion ROK activity was significantly greater in WBCs subjected to myocardial ir compared to the shamoperated mice administration of fasudil a rhokinase inhibitor significantly reduced the irinduced expression of the proinflammatory cytokines interleukin il cc motif chemoattractant ligand ccl and tumor necrosis factor tnfα in WBCs compared with saline as the VH furthermore fasudil decreased irinduced myocardial infarctionarea at risk ia and irinduced leukocyte infiltration in the myocardium interestingly ia in fasudiladministered mice with leukocyte depletion was similar to that in fasudiladministered mice ir also resulted in remarkable increases in the mrna expression C2 of the proinflammatory cytokines tnfα il and ccl in the heart inhibition of rhokinase activation in leukocytes has an important role in fasudilinduced cardioprotective effects hence inhibition of ROK may be an additional therapeutic MI for the treatment of acute coronary syndrome
|
MEDAL
|
a novel methodology is proposed for food specifications associated with the origin of food the methodology was tested on honey samples collected within the trace eu project the data were sampled in various regions in europe and analysed for the trace elements content the sampling sites were characterized by different geological origins such as limestone shale or magmatic we have chosen elements b na mg a k ca mn co ni cu zn rb sr and ba due to their influence on the separation of samples regarding the geology of the sampling CS a special architecture of an error backpropagation neural network so called bottleneck type of neural network was used to project the data into a d plane the data were fed into the NO input SL and then transferred through the NO hidden layer compared to a bottleneck to the nodes output mulation sequence and location on the temporal vulnerability in Epi and endocardial CS in an arterially perfused rabbit left ventricular wedge preparation at three basic cycle lengths bcl of and ms an S2 s was introduced at coupling intervals incremented by ms to scan the entire DI the results showed that the vulnerable window increased with the lengthening of bcl which ranged from ms at bcl of ms to ms at bcl of ms for endocardial and is a similar style for epicardial from ms at bcl of ms to ms at bcl of ms p the vulnerable window of endocardial was wider than that of epicardial p furthermore the window size for reentry was about smaller than that for unidirectional conduction block meanwhile we found that increasing the number of premature beats enlarged the vulnerable window markedly due to enhanced TDR tdr of IVT the vulnerable window is an ERP index to evaluate the increased risk for unidirectional conduction block therefore the AF that increase the vulnerable window are associated with the higher incidence of VVI arrhythmias
|
MEDAL
|
the effect of alterations in sodium transport on cell atp content and ph in the isolated perfused PCT tubule pct of the rabbit was examined stimulating sodium transport by the addition of luminal gl and alanine decreased cell atp from to mm n increased intracellular ph by n and increased cell volume by nlmm n blocking the sodium pump with m strophanthidin in tubules in which sodium transport had been stimulated increased cell atp from to mm n in parallel experiments the same dose of strophanthidin depolarized the basolateral membrane from to mv depolarized the transepithelial potential from to mv and reduced the basolateral membrane potassium transference number from to indicating a reduction in basolateral potassium conductance since strophanthidin caused a cell alkalinization of this latter effect cannot be due to changes of intracellular ph strophanthidin caused no NC in cell volume over the period studied suggesting that stretchactivated potassium channels are not involved either instead potassium conductance inhibition may be the result of the closure of atpsensitive potassium channels these same channels might thus be partly responsible for the increase in gK commonly observed during stimulation of sodium transport
|
MEDAL
|
effects of leachate addition on ammonia volatilization and no and co emissions from two different soils were investigated using the day laboratory incubation method at two levels of MC ammonia volatilization was dominated by soil ph and only occurred in alkaline clay soil where of soil ammonia could be lost the no emission from the ALP clay soil was one order of magnitude greater than that from the acidic sandy soil when either water or leachate was irrigated increasing the MC from waterfilled pore space wfps to wfps in the alkaline clay soil or the acidic sandy soil by either water or leachate irrigation increased the no emission by over twofold the co emission from each soil sample at the two wfpss was almost the same the co emission from the alkaline clay soil with leachate addition was lower than that from the acidic sandy soil with leachate addition and times higher than that from the alkaline clay soil with distilled water addition ammonia volatilization and no emission under leachate irrigation could be minimized by avoiding the excessively wet condition and by selecting the acidic sandy soil with low organic carbon and total nitrogen content
|
MEDAL
|
the statistical analysis of continuous longitudinal data may be complicated since quantitative C2 of bioassay cannot always be determined values beyond the limits of detection lod in the assays may not be observed and thus censored rendering complexity to the analysis of such data this article examines how both leftcensoring and right censoring of hiv plasma rna measurements collected for the T0 on aidsrelated nonhodgkins lymphoma arnhl in east africa affects the quantification of viral load and explores the NK PH of viral load measurements over time in arnhl patients receiving anticancer chemotherapy data analyses using MC em algorithm mcem are compared to analyses where the lod or lod left censoring value is substituted for the censored observations and also to other methods such as multiple imputation and maximum likelihood estimation for censored data generalized tobit regression simulations are used to explore the sensitivity of the results to changes in the MM parameters in conclusion the ARV treatment was associated with a significant decrease in viral load after controlling the effects of other covariates a simulation T0 with finite sample size shows mcem is the least biased method and the estimates are least CS to the censoring mechanism
|
MEDAL
|
we describe the mapping and ID of the gene for hereditary myopathy with LA hml hml is characterized by low PCS performance resulting in physical exertion that causes early exhaustion dyspnoea and palpitations using an AR mode of inheritance we mapped the trait to chromosome q with a maximum lod score of the mb diseasecritical region contained one obvious candidate geneiscuspecifying a protein involved in ironsulphur cluster assembly iscu is produced in two isoforms one cytosolic and one mitochondrial coded for by different splice variants of the iscu gene mutational analysis of all exon and intron sequences as well as bp of the promoter of the iscu gene revealed one intron mutation that was TPS for the disease haplotype the mutation is located in a region with homology to the interferonstimulated response PE isre but we could not see any effect of the mutation on expression levels in vitro or in vivo we did however observe a drastic difference in the splicing pattern between patients and controls in controls the mrna was as expected mainly in the mitochondrial form while in the patients a larger mrna transcript was predominant sequencing of the product revealed that the mutation activates cryptic splice CS in intron resulting in aberrant mrna containing bp of the intron to coobectomy for a carcinoma localized in the RUL
|
MEDAL
|
gata is the founding member of a transcription factor family that regulates growth and maturation of a diverse set of tissues gata is expressed primarily in hematopoietic cells and is essential for proper development of erythroid cells megakaryocytes eosinophils and mast cells although loss of gata leads to differentiation arrest and apoptosis of erythroid progenitors absence of gata promotes accumulation of immature megakaryocytes recently we and others have reported that mutagenesis of gata is an early event in down syndrome ds leukemogenesis acquired mutations in gata were detected in the vast majority of patients with acute megakaryoblastic leukemia dsamkl and in nearly every patient with transient myeloproliferative disorder tmd a preleukemia that may be present in as many as of infants with ds although the precise pathway by which mutagenesis of gata contributes to one is unknown these findings confirm that gata plays an important role in both normal and malignant hematopoiesis future studies to define the mechanism that results in the high frequency of gata mutations in ds and the role of altered gata in tmd and dsamkl will shed CS on the multistep pathway in HL-60 and may lead to an increased understanding of why children with ds are markedly predisposed to one
|
MEDAL
|
hypoglycemiainduced cerebral neuropathy can occur in patients with diabetes who attempt tight control of blood gl and may lead to cognitive dysfunction accumulating evidence from animal models suggests that hypoglycemiainduced neuronal death is not a simple result of GD but is instead the end result of a multifactorial process in particular the excessive activation of poly adpribose polymerase parp consumes cytosolic nicotinamide adenine CpG nad resulting in energy failure in this T0 we investigate whether lactate administration in the absence of cytosolic nad affords neuroprotection against hypoglycemiainduced neuronal death intraperitoneal injection of sodium llactate QTc arterial blood ph and blood lactate concentration after hypoglycemia lactate po without gl was not sufficient to promote electroencephalogram recovery from an isoelectric state during hypoglycemia however supplementation of glucose with lactate reduced neuronal death by â¼ in the hippocampus hypoglycemiainduced superoxide production and microglia activation was also substantially reduced by administration of lactate taken together these results suggest an intriguing possibility that increasing BB lactate following hypoglycemia offsets the decrease in nad due to overactivation of parp by acting as an alternative energy substrate that can effectively bypass glycolysis and be fed directly to the CAC to maintain cellular atp levels
|
MEDAL
|
critical flicker frequency cff of men mentally retarded whose mean age was yr and normal whose mean age was yr was measured under binocular viewing using the lafayette visual perception control with a display unit subjects had been previously tested for visual acuity and color blindness analysis showed a significant difference in cff between mentally retarded persons and normal individuals the former having lower cff than the latter this finding suggests lower perceptual sensitivity of the MR persons further research with provision for eeg recordings is suggested
|
MEDAL
|
a yearold man had undergone orchiectomy and chemotherapy for TC despite normalization of raised tumor marker C2 T3 postoperative chemotherapy computed tomographic scanning demonstrated multiple swellings of the paraaortic lymph nodes with extension from beneath the aortic arch to the bifurcation of the descending aorta open biopsies of the paraaortic lymph nodes disclosed mature teraroma without malignant cells the patient presented the typical features of mediastinal and RP growing teratoma syndrome a two stage resection of the PT was performed via laparotomy and left thoracotomy histological examination of the resected specimens revealed a mature teratoma component without malignant cells upon followup sixteen months later the patient was well and without recurrence
|
MEDAL
|
in consecutive children undergoing cardiac surgery two different types of fluid were given randomly for volume replacement in the prebypass period in group n low molecular weight hydroxyethyl starch solution lmwhes hes mean molecular weight da molar substitution and in group n albumin ha was infused from the induction of anaesthesia until the start of CP bypass cpb in addition to haemodynamic values various laboratory variables were measured before and T3 cpb until the morning of the st day T3 T0 the patients did not differ in diagnosis and conduct of cpb lowest rectal temperatures group sd degrees c group degrees c haemodynamic data map hr cvp antithrombiniii fibrinogen PLT count and coagulation variables were comparable between the groups until the st day T3 T0 postoperative BL and the use of HB or blood products were similar in all children ALB increased after infusion of Al g litre and was significantly greater until the end of the T0 compared with the lmwhestreated children colloid OP however was similar in the two CG and returned to baseline values on the st day T3 operation lmwhes group mm hg ha group mm hg postbypass urine SO and creatinine values also did not differ between the CG anaphylactic reactions were not observed in any of the patients it can be concluded that lmwhes solution can be used effectively and safely for volume replacement in the prebypass period in small children undergoing CS
|
MEDAL
|
much remains to be understood about the differential contributions from primary and secondary sensory cortices to sensoryguided decision making to address this issue we simultaneously recorded activity from neuronal ensembles in primary gustatory cortex gc and AA gustatory OFC ofc cortices while rats made a tasteguided decision between two response alternatives we found that before animals commenced a response guided by a tastant cue gc ensembles contained more information than ofc about the response alternative about to be selected thereafter while the animals response was underway the responseselective information in ensembles from both regions increased albeit to a greater degree in ofc in gc this increase depends on a representation of the taste cue guiding the animals response the increase in the ofc also depends on the taste cue guiding and other features of the response such as its spatiomotor properties and the behavioral context under which it is executed each of these latter features is encoded by different ensembles of ofc neurons that are recruited at TPS times throughout the response selection process these results indicate that during a tasteguided decision task both primary and secondary gustatory cortices dynamically encode different types of information
|
MEDAL
|
RO and nanofiltration nf employ composite membranes whose ultrathin barrier layers are significantly more permeable to water than to salts although solutiondiffusion models of salt transport through barrier layers typically assume ubiquitous electroneutrality in the case of ultrathin selective skins and low ion partition coefficients spacecharge regions may occupy a significant fraction of the membrane barrier layer this work investigates the implications of these deviations from electroneutrality on salt transport both immobile external surface charge and unequal cation and anion solvation energies in the barrier SL lead to regions with excess mobile charge and the size of these regions increases with decreasing values of either feed concentrations or ion partition coefficients moreover the low concentration of the more excluded ion in the spacecharge region can greatly increase resistance to salt transport to enhance salt rejection during nf these effects are especially pronounced for CM with a fixed external sigma whose sign is the same as that of the more excluded ion in a salt because of the spacecharge regions the barrierlayer resistance to salt transport initially rises rapidly with increasing barrier thickness and then plateaus or even declines within a certain thickness range this trend in resistance implies that thin defectfree barrier layers will exhibit higher salt rejections than thicker layers during nf at a fixed transmembrane pressure deviations from electroneutrality are consistent with both changes in nf salt rejections that occur upon changing the sign of the membrane fixed external surface charge and cacl rejections that in some cases may first decrease then increase and then decrease again with increasing cacl concentrations in nf feed solutions
|
MEDAL
|
during the development of the rat sciatic nerve extensive proliferation of glial cells occurs and there is a very substantial rearrangement of the cytoarchitecture as axons and schwann cells assume relationships which lead to the formation of the myelinated and unmyelinated axons characteristic of AD nerve the maturatiog and drinking chief among these Az was a continuous descending pathway from the ventral tegmental area through the lateral tegmentum to the cuneiform and PB nuclei smaller andor less reliable increases above controls were found in the dorsomedial caudateputamen the PL zona incerta the anterior lateral CG the caudal linear nucleus the laterodorsal tegmental nucleus the pontine tegmental nucleus and a previously undescribed pathway lying medially between the pontine medial lemniscus and CP these Az especially the lateral tegmental and PB zone are the most likely candidates for the pathways andor destinations of the directly excited efferents or SF of passage that constitute the first link in the elicitation of gnawing eating and drinking by lateral HS since SS and exploratory activity were elicited by control as well as exp electrodes they are probably dependent on other projections among those affected similarly in both groups
|
MEDAL
|
skeletal involvement of nonhodgkins lymphoma is found in in hodgkins disease in PLB has an incidence of among all nonhodgkins lymphoma the occurrence of skeletal lesions is higher in infants and children than in adults skeletal lesions caused by HD and nonhodgkins lymphoma are mostly seen in the axial skeleton including the skull whereas the primary lymphoma of bone seems to prefer a more peripheral site the aggressiveness of the RT growth can be measured by the method of lodwick by judging the edge characteristic the penetration of the cortex the periostal and sclerotic reaction examples illustrate this method conventional radiographs need only be performed when there is reason to believe a lesion is located in an area of structural importance such as the neck of the femur and in cases of skeletal pain of unknown origin
|
MEDAL
|
the objective of this T0 was to investigate the effect of losartan nk dup a new selective angiotensin ii receptor antagonist on insulin sensitivity and sympathetic nervous system activity in patients with severe primary hypertension five patients with a record of diastolic blood pressure dbp or mmhg currently either untreated or with dbp mmhg on antihypertensive treatment were examined in an open study with the euglycemic gl clamp examination before and T3 being treated with losartan for an average of weeks the Rd increased from to mgkg x min p during treatment with losartan the insulin sensitivity index Rd rate divided by mean insulin concentration during clamp increased from to arbitrary units p PNA decreased from to nmoll p while plasma adrenaline was unchanged vs nmoll ns mean blood pressure decreased from to mmhg p and HR rate was unchanged during treatment with losartan thus antihypertensive treatment with the new selective angiotensin ii receptor antagonist losartan seems to improve insulin sensitivity a decrease in plasma noradrenaline on LOS suggests a sympathicolytic effect which together with vasodilation may explain the fall in blood pressure and the improvement in insulin sensitivity
|
MEDAL
|
the purpose of this study was to determine whether exercise training alters vasomotor CR of rat CAs in vitro ISO microvessel techniques were used to evaluate vasomotor properties of proximal left anterior artery rings ring per animal from exercisetrained rats et n subjected to a wk TM training protocol mmin incline hday dayswk and control rats c n restricted to cage activity no differences in passive lengthtension characteristics or internal diameter and micron were observed between vessels of c and et rats CR curves to k mm prostaglandin falpha m and norepinephrine were unaltered p in coronary rings from et rats compared with c rats however lower values of the concentration producing of the maximal contractile response in rings from et rats p suggest that contractile sensitivity to norepinephrine was enhanced vasorelaxation responses to sodium nitroprusside m and adenosine m were not different p between vessels of c and et rats however relaxation responses to the endotheliumdependent vasodilator acetylcholine ach m were significantly blunted p in coronary rings from et animals maximal ach relaxation averaged and respectively in vessels of c and et CG in additional experiments two coronary rings proximal and distal were isolated from each c n and et n animal proximal coronary artery rings from et animals demonstrated decreased EC50 responses to ach however achmediated relaxation of distal coronary rings was not different between c and et CG ngmonomethyllarginine inhibitor of nitric oxide synthase blocked ach relaxation of all rings larginine substrate for nitric oxide synthase did not improve the blunted ach EC50 in PT PD rings from et rats these studies suggest that exercisetraining selectively decreases endotheliumdependent ach but not endotheliumindependent sodium nitroprusside EC50 responses of rat PT coronary arteries endotheliumdependent EC50 of distal coronary arteries is unaltered by training
|
MEDAL
|
seventysix children aged years who were known to be positive for hepatitis b surface antigen and hepatitis b e antigen in SS for at least months and who had biopsyproven CH have been followed longitudinally for years mean years twentythree of them are now young adults eight patients had acute type b hepatitis months before entering the study while patients came to observation during a CP at the beginning of followup all children were positive in serum for hepatitis b virus dna and had chronic active SH associated with cirrhosis in two cases during followup patients remained SH b e antigenpositive most with unchanged biochemical and histological features the other cases seroconverted to SH b e antibody and cleared SH b virus dna from SS including of AH at presentation T3 SC alanine aminotransferase C2 normalized in all patients and remained normal in patients throughout a mean observation period of years five of these children including of with previous acute SH eventually cleared hepatitis b surface antigen from their sera finally patients experienced a mild increase of alanine aminotransferase C2 several months T3 seroconversion in the absence of SH b virus replication or of delta virus superinfection clinical and virological parameters did not significantly differ between patients with or without acute onset however seroconversion occurred earlier and the rate of hepatitis b surface antigen clearance was greater in the former than in the latter group the present data indicate that approximately two thirds of children with SH b e antigen and SH b virus dnapositive chronic hepatitis clear hepatitis b virus dna from their sera before reaching adulthood after termination of viral replication most patients achieve a sustained biochemical remission suggesting the disappearance of disease activity reactivation of virus replication T3 SH b e antibody seroconversion has never been observed in this series although mild alanine aminotransferase level CA could be detected in a minority of cases
|
MEDAL
|
flux balance analysis fba with genomescale metabolic network models gsmnm allows systems level predictions of metabolism in a variety of organisms different types of predictions with different accuracy levels can be made depending on the applied experimental constraints ranging from measurement of exchange fluxes to the integration of mRNA data metabolic network modeling with model organisms has pioneered method development in this field in addition model organism gsmnms are useful for basic understanding of metabolism and in the case of animal models for the study of metabolic human diseases here we discuss gsmnms of most highly used MM organisms with the emphasis on recent reconstructions
|
MEDAL
|
diagnostic therapy is often recommended for children before PCS management of the velopharyngeal structures there is however limited information about the effectiveness of such intervention programs this study describes the changes that occurred in a yearold childs production of speech during a period of diagnostic therapy and the changes that occurred following the fitting of a prosthesis the mother served as the primary intervener guided by a speechlanguage pathologist the mother was able to change the childs speech so that more of her productions were at a correct place of articulation after structural management nasal and glottalized productions disappeared from the childs speech but glottal stops did not
|
MEDAL
|
we have recently shown that systemic administration of a superoxide dismutase mimetic tempol resulted in decreases in MAP pressure and HR rate along with a reduction in renal SNA rsna it has also been shown that these parameters are significantly increased by SVR administration of a superoxide dismutase inhibitor diethyldithiocarbamic detc indicating a potential role of reactive oxygen species in the regulation of rsna in this T0 we examined the effects of local administrations of hydroxytetramethylpiperidinenoxyl tempol and detc on rsna in anesthetized rats either tempol or detc was directly administered onto the renal sympathetic SN located between the electrode and ganglion local application of tempol microl to moll n resulted in dosedependent decreases in integrated rsna by at moll without alterations in mean arterial pressure and HR rate in contrast detc microl to moll n increased rsna dosedependently the responses of rsna to tempol and detc were significantly greater in spontaneously hypertensive rats than in NR n respectively local application of sodium nitroprusside mmoll or ngnitrolarginine methyl ester moll altered neither basal rsna nor tempolinduced reductions in rsna n and respectively a Kv channel blocker aminopyridine moll significantly decreased basal rsna by and completely prevented detcinduced increases in rsna n these results suggest that ROS species play a role in the regulation of peripheral sympathetic nerve activity and that at least part of this mechanism is mediated through voltagegated potassium channels
|
MEDAL
|
current understanding of electrostatics in water is based on meanfield theories like the poissonboltzmann formalism and its approximations which are routinely used in colloid science and computational biology this approach however breaks down for highly charged systems which exhibit counterintuitive phenomena such as overcharging and likecharge attraction models of counterion correlations have been proposed as possible explanations but no exp comparisons are available here collective dynamics of counterions that mediate likecharge attraction between factin filaments have been directly observed in aqueous solution using highresolution inelastic xray scattering down to molecular lengthscales we find a previously undescribed acousticlike phonon mode associated with correlated counterions the excitation spectra at high wavevector q reveal unexpected dynamics due to ions interacting with their cages of nearest neighbors we examine this behavior in the context of intrinsic charge density variations on factin the measured speed of sound and collective EC50 rates in this liquid agree surprisingly well with simple MM calculations
|
MEDAL
|
NE serotonergic and histaminergic SN are continuously AS during waking reduce discharge during nrem sleep and cease discharge during rem sleep cataplexy a symptom associated with narcolepsy is a waking state in which muscle tone is lost as it is in rem sleep while environmental awareness continues as in alert waking in prior work we reported that during cataplexy NE neurons cease discharge and serotonergic neurons greatly reduce activity we now report that in contrast to these other MA remoff cell CG histamine SN are AS in cataplexy at a level similar to or greater than that in quiet waking we hypothesize that the activity of histamine cells is linked to the maintenance of waking in contrast to activity in NE and serotonergic SN which is more tightly coupled to the maintenance of muscle tone in waking and its loss in rem sleep and cataplexy
|
MEDAL
|
br is a multiherbal formula that contains twelve medicinal herbs we investigated the effects of oral administration of br to WR on a learning and SM using a PA test and b cell proliferation in the DG dg of the hippocampus using immunohistochemical analysis of bromodeoxyuridine brdu expression in the PA test the PR time of the brtreated group was significantly longer than that of the control group vs s respectively n p there were significantly more brduimmunoreactive cells in the dg in the brtreated group than in the control group vs cells per dg respectively n p these results suggest that the administration of br not only improves learning and SM but also increases cell proliferation in the dg of the rat hippocampus
|
MEDAL
|
bacterial vaginosis bv is characterised by depletion of the normal lactobacillus spp and overgrowth of commensal anaerobic bacteria we investigated the composition of vaginal microbiota and their association with bv in women of reproductive age vaginal samples from women were analysed whereby n patients had normal flora and n were diagnosed with bv the remaining patients were diagnosed with having either intermediate flora n or dysbacteriosis n the reported results show that pathogens are associated with bv this knowledge will further expand our understanding of events leading to bv which may lead to more ERP prevention and treatment strategies
|
MEDAL
|
whether the incidence of coronary HR disease chd is related to a decrease in total antioxidant capacity tac has not yet been completely clarified we assessed tac of BS in a group of men with chd aged years and in agematched peers without chd two spectrophotometric methods were applied to assess tac ferric reducing ability of serum tacfras and diphenylpicrylhydrazyl tacdpph tests in the chd group multivariate analysis revealed that uric acid ua triglycerides and systolic blood pressure contributed independently to the tacfras variance tacdpph was favorably predicted by ua concentration but negatively so by current smoking and glucose levels in men without chd ua was the only independent determinant of both tacfras and tacdpph presence of chd was not an independent predictor of tacobserved betweengroup differences higher tac in chd patients disappeared T3 adjustment for other confounders we conclude that ua is the main determinant of tac of BS in men tac is not directly influenced by age or chd but is related to several indices of overweightobesity and laboratory measures of metabolic syndrome especially in patients with chd
|
MEDAL
|
a high cure rate of ovarian germ cell PT was achieved by establishment of a cisplatincontaining regimen we encountered a rare case of a yearold nulligravida japanese woman with systemic TE following anticancer chemotherapy with cisplatin etoposide and bleomycin in ovarian germ MCT she suffered from brain infarction multiple embolisms in the bilateral pulmonary artery and pelvic vein and a broad range of deep vein thromboses in the RA extremities vein when thrombosis is detected during anticancer chemotherapy we must investigate whether the presence of thrombosis is SVR or local
|
MEDAL
|
a tetramer MM for hiv integrase in with dna representing bp of the u and u long terminal repeats ltr termini was assembled using structural and biochemical data and MD it predicted CAA residues on the enzyme surface that can interact with the ltr termini a separate structural alignment of hiv SSV siv and avian sarcoma virus asv ins predicted which of these residues were unique to determine whether these residues were responsible for specific recognition of the ltr termini the amino acids from asv in were substituted into the structurally equivalent positions of hiv in and the ability of the chimeras to process u hiv or asv duplex oligos was determined this analysis demonstrated that there are multiple amino acid contacts with the ltrs and that substitution of asv in amino acids at many of the analogous positions in hiv in conferred partial ability to cleave asv substrates with a concomitant loss in the ability to cleave the homologous hiv substrate hiv in residues that changed specificity include val ser lysile glyval and hisleu because a chimera that combines several of these substitutions showed a specificity of cleavage of the u asv ATP closer to wild type asv in compared with chimeras with individual amino acid substitutions it appears that the sum of the in interactions with the ltrs determines the specificity finally residues ser and val in hiv in are among those that NC in enzymes that develop resistance to naphthyridine carboxamide and diketo acidrelated inhibitors in cells thus amino acid residues involved in recognition of the ltrs are among these positions that change in development of DR
|
MEDAL
|
polyethylene oxide peografted polyurethane pupolystyrene ps interpenetrating polymer networks ipns were synthesized the effects of the mobile pendant peo chains with their microphase separated structure on bloodcompatibility were investigated the morphology of both the fracture surface as well as the top surface indicate that the size of the dispersed domainnning in this latter case the period of the cycle was approximately h in synchronized conditions hypoxia almost eliminated the tb circadian oscillation because of the blunting of the tb rise during the dark phase on return to normoxia tb rapidly increased toward the maximum normoxic values and the normal cycle was then reestablished in hypoxia the amplitude of the activity and vo oscillations averaged respectively and of normoxia in freerunning conditions on return to normoxia the rhythm was reestablished at the expected phase of the cycle hence the action of hypoxia was not on the clock itself but probably at the Thy centers of thermoregulation hyperoxia o or hypercapnia co had no significant effects on circadian oscillations indicating that the effects of hypoxia did not reflect an undifferentiated response to changes in environmental gases modifications of the metabolism and tb rhythms during hypoxia could be at the origin of sleep disturbances in cardiorespiratory patients and at high ALT
|
MEDAL
|
the purpose of this T0 was to examine the hypothesis that the calcium channel blocker diltiazem modulates catecholamineinduced arrhythmias through cns mechanisms rats that had catheters previously inserted into the lateral cerebral ventricle and FA artery received diltiazem or microgramskg or the diluent into the lateral cerebral IVT icv epinephrine was infused to produce arrhythmias the onset of VAs premature ventricular complexes occurred at a significantly p less than greater dose of epinephrine after diltiazem compared to the control group and in a dosedependent manner with the mean sem dose of EN being and microgramskg in the CG treated with and microgramskg of diltiazem and the control groups respectively the development of fatal arrhythmias mainly VTs occurred at significantly p less than greater concentrations of epinephrine with diltiazem and microgramskg and microgramskg respectively compared to controls microgramskg endogenous opioids of the mutype were implicated in this action of diltiazem because the mu opioid antagonist naloxone mgkg iv significantly p less than antagonized the antiarrhythmic effects of centrally administered diltiazem and the mu opioid agonist dago icv did not further enhance the suppression of epinephrineinduced arrhythmias produced by diltiazem microgramskg atropine sulfate which crosses the bloodbrain barrier and atropine methylnitrate which does not enter the brain each at mgkg iv produced an equal and significant antagonism of the effect of diltiazem microgramskg that was less than that of naloxone the combination of naloxone plus atropine sulfate completely prevented the effect of diltiazem microgramskg on arrhythmias the antiarrythmic action of diltiazem could not be explained by alteration of the blood pressure or HR rate response to epinephrine the results suggest that a calcium channels on SN in the cns play an important role in the modulation of epinephrineinduced cardiac arrhythmias b diltiazem can suppress arrhythmias through cns mechanisms c activation of the parasympathetic NS mediates some of the effect of diltiazem but d the mechanism of action of diltiazem is modulated through endogenous opioids
|
MEDAL
|
WM refers to a system to temporary holding and manipulation of information previous studies suggested that local field potentials lfps and spikes as well as their coordination provide potential mechanism of working memory popular methods for lfpspike coordination only focus on the two modality signals isolating each channel from multichannel data ignoring the entirety of the networked brain therefore we investigated the coordination between the lfp network and spike network to achieve a better understanding of working memory multichannel lfps and spikes were simultaneously recorded in rat prefrontal SC via microelectrode array during a ymaze WM task functional connectivity in the lfp network and spike network was respectively estimated by the DTF dtf and MLE mle then the coordination between the two networks was quantified via canonical correlation analysis cca the results show that the canonical correlation cc varied during the working memory task the cccurve peaked before the choice point describing the coordination between lfp network and spike network enhanced greatly the cc value in WM showed a significant higher level than intertrial interval our results indicate that the enhanced canonical correlation between the lfp network and spike network may provide a potential network integration mechanism for WM
|
MEDAL
|
in this T0 relationships between normalized difference vegetation index ndvi and plant winter wheat nitrogen content pnc and between pnc and grain protein content gpc were investigated using multitemporal moderateresolution imaging spectroradiometer modis data at the different stages of winter wheat in linfen shanxi p r china the anticipating model for gpc of winter wheat was also established by the RPA of ndvi at the different stages of winter wheat the results showed that the spectrum models of pnc passed f test the ndvi regression effect of pnc model of irrigated winter wheat was the best and that in dry land was ndvi the pnc of irrigated and dry land winter wheat were significantly p and positively correlated to gpc both of protein spectral anticipating MM of irrigated and dry land winter wheat passed a significance test p multiple anticipating models mam were established by ndvi from two periods of irrigated and dry land winter wheat and pnc to link gpc anunding AF such as high stress or drug side effects that can mask or interact with the effects of milk deprivation per se given these limitations the development of new models of ew may provide useful information regarding the impact of a shortened period of breastfeeding on the endocrine and nervous systems both during OD and at adulthood using a MM of ew in which lactating wistar rat dams are wrapped with a bandage to block access to milk during the last three days of lactation we have recently shown that the adult F1 presented higher body mass hyperphagia hyperleptinemia leptin as well as insulin resistance and higher adrenal catecholamine content at adulthood here we used this ew model which involves no pharmacological treatment or maternal separation to analyze anxietylike noveltyseeking and memorylearning behavioral traits in the AD male F1 to that end animals were tested in the elevated plus maze in the hole board arena and in the radial arm WM except for an increased number of rearing events a measure of vertical activity no other behavioral differences were observed between ew and control animals the contrasting behavioral results between the three ew models may be associated with differences in hpa axis function in the F1 at weaning since it has been observed that bandaging does not affect corticosteronemia while MS and pharmacological ew increase it
|
MEDAL
|
the article deals with the possibility of chlorophyll and chlorophyllase immobilization on methylaerosil and also with the effect of this carrier on the enzymesubstrate interaction chlorophyll and chlorophyllase are shown to be able to hydrophorbic interaction it is established that the ultimate saturation of methylaerosil with chlorophyll is mg per mg of the carrier the chlorophyllase reaction is the most AS under conditions when the enzyme and ATP are in soluble states the hydropholic IB of chlorophyll with methylaerosil decreases the intensity of its hydrolysis for the period of reaction and the preliminary immobilization of chlorophyllase on methylaerosil inhibits the reaction to a still greater extent this evidences for the significance of the reaction system organization for interaction of chlorophyllase with chlorophyll
|
MEDAL
|
the present study was designed to determine if either the testes or thyroid plays a role in the blunted gh secretion observed in the obese male rat analysis of individual gh secretory profiles in adult lean and obese zucker rats revealed a severe attenuation of both mean gh C2 and individual gh PP amplitudes in obese rats as well as a significant lowering of circulating testosterone levels normalization of the testosterone levels by the use of sc silastic capsules elevated but did not normalize gh PP amplitudes in obese animals further the gh response to either rat ghreleasing factor mukg or morphine mgkg were reduced in obese male rats in contrast the thyroid axis showed minimal change in obese animals a slight reduction in FT levels in SS was observed while free and total t and total t were normal in obese rats further mean circulating tsh levels and the tsh response to ngkg trh were not altered in fat rats thus the reduced gh secretion observed in the obese rat is not paramount to an alteration in either gonadal or thyroid function this alteration of the gh secretory axis may not be attributable to one factor but more likely caused by a number of concomitant deficits which may act in concert to manifest impaired gh secretion
|
MEDAL
|
in our initial experience of four cases from march to november large paraesophageal hernias were repaired by conventional PC of the hiatus with interrupted nonabsorbable sutures adding a degrees fundoplication in all four cases the hernia recurred subsequently we modified the procedure the technique and results are described from march to may patients with paraesophageal hernia following a previous nissen procedure underwent elective laparoscopic repair in all patients a tensionfree hiatoplasty and a floppy degrees fundoplication were performed the hiatal defect was repaired with a polypropylene mesh fixed to the diaphragm by staples a gastrophrenic anchorage procedure was added in the eight patients undergoing suributions and the relative speeds of error and correct response times aspects of the data about which models designed to account for brinley plots are mute we conclude by endorsing a research RPA that applies explicit models to response time data in aging in order to use the parameters of the MM to interpret the effects of aging
|
MEDAL
|
a GA alloptical implementact cysts result from an abnormality in regression of the müllerian system they may occasionally give rise to PS we report an unusual case of AUR in an old man caused by a giant müllerian duct cyst a yearold man presented with of acute urinary retention T3 bladder drainage digital rectal examination found a large soft supraprostatic mass TRUS and computed tomography scans revealed a large multilocular retrovesical cystic mass the patient underwent open surgical resection of the cyst histologically the cystic lesion was lined with stratified cubocolumnar cells consistent with a müllerian duct cyst acute UR in the elderly is not always related to prostatic diseases other causes even congenital ones may be involved
|
MEDAL
|
a yearold man underwent slow pathway ablation for slowfast AVN reentrant ART following the procedure he felt palpitations while swallowing and swallowinginduced AT was diagnosed swallowinginduced atrial ART arose from the right atriumsuperior VC junction and was cured by CA T3 the procedure the patients HRV changed significantly indicating suppression of parasympathetic nerve activity in this case swallowinginduced atrial ART was related to the vagal nerve reflex analysis of HR rate R2 may be helpful in elucidating the mechanism of swallowinginduced AT
|
MEDAL
|
the effects of baroreceptor stimulation produced by a sudden rise in arterial pressure on respiratory DP were studied at various C2 of paco and pao in anesthetized cats a sudden rise in arterial pressure decreased both tidal volume and RF for a given increase in arterial pressure the decrease in tidal volume was constant at all levels of paco and pao examined whereas the decrease in RF became progressively lower as the level of chemical drive increased regardless of the type of chemical stimuli analysis of respiratory duration revealed that the decrease in respiratory frequency during baroreceptor stimulation was solely due to prolongation of expiration these observations were obtained in the intact as well as in the vagotomized animals but were not seen T3 bilateral sinus nerve section they suggest that the central respiratory mechanisms controlling RF are directly involved in the reduction in respiratory frequency during baroreceptor stimulation
|
MEDAL
|
the purpose of this study was to investigate the relationship between dynamic sitting balance and walking ability in stroke patients patients were evaluated using FIM fim and those with a locomotion score of or below were categorized into the dependent walking and below group dependent group and those with a score of or above were categorized into the independent walking group independent group they were asked to take a sitting position at the center of an unstable platform seesaw the experimenter passively tilted the unstable platform to the paretic side or nonparetic side by degrees in the frontal plane the unstable platform was released suddenly with the experimenter supporting the platform with both hands and the subjects were asked to control their posture so that their body axis came to the vertical position and the platform came to the horizontal position in the frontal plane as a result the body axis of left hemiplegic patients significantly tilted to the nonparetic side on the other hand in the dependent group in the RA as well as left hemiplegic patients the patients heads were tilted to the side from which tilting was started though not significantly in both conditions this study demonstrated that patients with poor walking CF have a deviated verticality of the head and the body axis we surmise that patients whose body axis tilts towards the nonparetic side during a dynamic balance maintenance task like this test would havter onset follow up T0 revealed recovered satisfactory were persistently disabled died during admission to hospital guillainbarre syndrome showed seasonal CSD and high frequency of axonal forms IVIG was more effective than plasma exchange poor outcomes were likely due to severe condition required mechanical ventilation and axonal forms it is crucial to timely identify rare SCV of gbs which recover with AGA treatment
|
MEDAL
|
visual and BS auditory evoked potentials vep and baep respectively and QEEG were studied in patients with baltic progressive myoclonus epilepsy pme the T0 demonstrated significantly delayed vep latencies but normal amplitudes in baltic pme baeps showed slight but significant prolongation in central conduction time quantitative eeg revealed diminution of beta and alpha activity and accentuation of theta and delta activity the slowing in vep latencies is suggested to be due to impaired synaptic transmission and to reflect dopaminergic dysfunction in baltic pme we conclude that there is a multimodal disturbance in sensory projections to cortical areas in baltic pme the results give further evidence that nondemyelinating disordersbut with synaptic transmission defectscan produce changes in evoked potentials the changes in epileptic BB are not confined to hyperexcitable epileptic neurons but more widespread electrophysiological phenomena are produced
|
MEDAL
|
prognostic indicators of shortterm outcome were identified in women with the dsmiiir diagnosis of bulimia nervosa who participated in a weekly session structured cognitivebehavioral outpatient group program prior to treatment all subjects completed the computerized DIS dis the moos FES fes the diagnostic survey for EDs dsed the BDI bdi the hopkins symptom checklist revised sclr the bulimic cognitive distortions scale bcds the eating disorders inventory edi and the bulimic symptoms checklist bscl the latter three scales were readministered on completion of the week group symptom improvement was assessed by examining percentage reduction in binge frequency purge frequency and summed scores for the edi subscales bulimia drive for thinness and BD the only significant predictor of improvement in binge frequency and bulimic cognitions was family environment conflicted controlling and overorganized family environments appear to impede both reductions in binge frequency and changes in bulimic cognitions reduction in vomit frequency was associated with weight PH and with laxative or diuretic use the implications for planning psychotherapeutic interventions in bulimia nervosa are discussed
|
MEDAL
|
the activity of i to be administered as therapy to patients with thyroid disease is usually calculated from h radioiodine uptake and thyroid volume the aim of the present T0 was to compare thyroid volume measured by scintigraphy and ultrasonography to evaluate the impact of these methods on the calculated i dose forty patients with diffuse toxic goiter and with multinodular toxic or nontoxic goiter were investigated on the same day thyroid volume was measured by ultrasonography using transverse scans at mm intervals and by scintigraphy using either the ellipsoid formula sce symbol see text x height x width x depth or the himanka formula sch x planimetric surface in pixels with ultrasonography the size of diffuse goiters was smaller than that of nodular goiters median values and range ml and ml respectively p both scintigraphic methods however failed to demonstrate a significant difference between diffuse and nodular goiter size in patients with diffuse goiter thyroid volume measured by sce did not differ from that measured by ultrasonography whereas thyroid size was overestimated by using the himanka formula in contrast in patients with nodular goiter thyroid volume measured by sch did not differ from that measured by ultrasonography ticulin distribution in the ovaries during zygotic embryogenesis showed that calreticulin biosynthesis started tissue specifically with a high abundance in the endothelium of the integument in the ovules followed by calreticulin accumulation in the embryo proper and in the associated endosperm at the late globular stage of embryogenesis using immunogold labeling calreticulin was intracellularly localized with a high abundance to the golgi compartment and to patches on the surface of dividing protoplasts smaller amounts were found in the endoplasmic reticulum and plasma CM the PET role of calreticulin in posttranslational processing and translocation processes apart from its postulated function in cellular ca homeostasis is discussed
|
MEDAL
|
in recent years the health care reform discussion in the united states has focused increasingly on the dual goals of costeffective delivery and better patient outcomes a number of new conceptual models for health care have been advanced to achieve these goals including two that are well along in terms of practical development and implementationthe patientcentered medical home pcmh and accountable care organizations acos at the core of these two emerging concepts is a new emphasis on encouraging physicians hospitals and other health care stakeholders to work more closely together to better coordinate patient care through integrated goals and data sharing and to create teambased approaches that give a greater role to patients in health care decisionmaking this RPA aims to achieve better health outcomes at lower cost the pcmh MM emphasizes the central role of primary care and facilitation of partnerships between patient physician family and other caregivers and integrates this care along a spectrum that includes hospitals specialty care and nursing homes accountable care organizations make physicians and hospitals more accountable in the care system emphasizing organizational integration and efficiencies coupled with outcomeoriented performancebased medical strategies to improve the health of populations the aco model is meant to improve the value of health PCS controlling costs while improving SQ as defined by outcomes safety and patient experience this document urges adoption of the pcmh model and acos but argues that in order for these new paradigms to succeed in the long term all sectors with a stake in health care will need to become better aligned with themincluding the employer community which remains heavily invested in the health outcomes of millions of americans at present acos are largely being developed as a part of the medicare and medicaid systems and the pcmh model is still gathering momentum and evolving among physicians but the potential exists for implementation of both of these concepts across a much broader community of patients by extending the wellconceived integrative concepts of the pcmh model and acos into the workforce via occupational and environmental medicine oem physicians the power of these concepts would be significantly enhanced occupational and environmental medicine provides a wellestablished infrastructure and parallel strategies that could serve as a force multiplier in achieving the fundamental goals of the pcmh model and acos in this paradigm the workplacewhere millions of americans spend a major portion of their daily livesbecomes an essential element next to communities and homes in an integrated system of health anchored by the pcmh and aco concepts to be successful oem physicians will need to think and work innovatively about how they can provide todays employer health servicesranging from primary care and preventive care to workers compensation and disability managementwithin tomorrows pcmh and aco models
|
MEDAL
|
the most common craniofacial malformation in the newborn is the orofacial cleft consisting of CL with or without CP and isolated cleft palate given its prevalence it is important to understand the etiology of the deformity medical management prior to surgical correction surgical techniques and timing
|
MEDAL
|
a high qfactor photonic MW filter showing tuning and reshaping capabilities and based on stimulated brillouin scattering is demonstrated the filter bandpass can be continuously tuned changing the microwave FEO used to generate the pump SP and the filter shape can be modified by modulating the microwave tone a single bandpass over the MW spectrum can be obtained by using singlesideband suppressed carrier modulation experimental results demonstrate the wide tuning range of the filter its reshaping capability and q factor of
|
MEDAL
|
twelve storm events were surveyed at shilipu catchment in wuhan city through threeyear monitoring regime the flow discharges total suspended solids tss COD cod TN tn and total phosphorus tp in runoff were measured to study the mechanism of urban stormwater runoff pollution the relationship between the event pollution load and the antecedent dry weather period was identified to discuss the influence of the urban surface sanitation management T0 of sewer pipe maintenance and rainfall characteristics on the urban stormwater runoff pollution it was found that the antecedent dry weather period and runoff amount were the important determining AF in the generation of urban stormwater runoff pollution the event pollution load was positively correlated to the antecedent dry weather period between two rainfall events r p it was the most important hydrological factor influencing the events pollution loads the best regression equation to estimate pollution load for storm events was developed based on the antecedent dry weather period and runoff depth source control including improving urban street sweeping activities and T0 of sewer pipe maintenance should be made to reduce the amount of available pollutant over the dry days it is important alternative to control urban stormwater runoff pollution for hanyang district
|
MEDAL
|
receptormediated endocytosis is the most TPS pathway for macromolecules and macromolecular complexes generally designated as ligands to enter cells upon IB to their TM receptors the ligands enter endocytic LDV that fuse with each other giving rise to the socalled early endosomes the sorting of ligandreceptor complexes internalized in these endosomes depends on their nature metabolic receptors are recycled back to the plasma membrane while signaling receptors and their ligands eg receptor IMT kinases or receptors associated with tyrosine kinase are delivered to internal LDV of the multivesicular late endosomes and finally are degraded T3 interaction with lysosomes during these processes endosomes undergo translocation from the cell periphery to the juxtanuclear region which is accompanied by multiple fusion invagination tabulation and membrane fission events this review considers modern concepts of the sorting mechanisms of ligandreceptor complexes the crosstalk between endosomes microtubules and actin and the role of this crosstalk in endosome maturation
|
MEDAL
|
carcinoid tumors are nonpancreatic digestive neuroendocrine PT well differentiated most of time they are developed from enterochromaffin cells of the digestive tract most of them are localized in ileum rectum stomach and appendix only bronchi are a frequent extradigestive localization to most of these PT are asymptomatic and discovered incidentally during a LAP or AA to the exploration of a carcinoid syndrome from a case report and a recent review of literature diagnosis histology and treatment of small bowel carcinoid tumors are summarized
|
MEDAL
|
pericardial effusion has been reported to occur in up to of patients with aids many different etiologies for pericarditis have been described in some instances such infections have caused severe hemodynamic complications and lifethreatening conditions mycobacterium kansasii is known to cause pulmonary and disseminated infections in ICP including those with aids however to the best of our knowledge pericarditis due to an infection with this organism has not been described we report the first three cases of m kansasii pericarditis in patients with aids
|
MEDAL
|
ischemiareperfusion ir injury induces endoplasmic reticulum er AS and cell death bax inhibitor bi is an evolutionarily conserved er protein that suppresses cell death and that is abundantly expressed in both CL and kidney we explored the role of bi in protection from er stress and ir injury by using bi KO mice employing models of transient hepatic or renal artery occlusion compared to wildtype bi mice bi KO subjected to hepatic ir injury exhibited these characteristics i increased histological injury ii increased SS transaminases indicative of more hepatocyte death iii increased percentages of tunelpositive hepatocytes iv greater elevations in ICE activity and v more activation of er SPs inositolrequiring enzyme and activating transcription factor and greater increases in expression of er SPs CHOP and spliced xbp protein moreover hepatic ir injury induced elevations in bi mrna in wildtype CL suggesting a need for bi gene induction to limit tissue injury similar sensitization of kidney to er AS and ir injury was observed in bi mice we conclude that bi provides endogenous protection of liver and kidney from er AS and ir injury analysis of components of the bidependent pathway for protection from ir injury may therefore reveal new strategies for organ preservation
|
MEDAL
|
the ciliary zonule in the eye also known as zinns zonule is composed of oxytalan SF which are bundles of microfibrils consisting mainly of fibrillin however it is still unclear which of the microfibrilassociated molecules present in the ciliary zonule controls oxytalan SF microfibrilassociated glycoprotein magp is the only microfibrilassociated molecule identified in the human ciliary zonule in the present study we used sirna against magp in cultures of human nonpigmented ciliary epithelial cells to examine the extracellular deposition and appearance of fibrillin employing western blotting and immunofluorescence magp suppression led to a reduction of fibrillin deposition immunofluorescence also confirmed that rnaimediated downregulation of magp led to suppression of fiber development these results suggest that magp plays a crucial role in the extracellular deposition of fibrillin during formation of the human ciliary zonule
|
MEDAL
|
the purpose of the present T0 was to determine the potential consequences to the IS of the combined SE of rats to stressor and morphine within min following either morphine mgkg i.t. or restraint AS min maximal analgesic responses as measured by tailflick assay were observed however only morphine treatment was accompanied by a significant suppression in mitogenstimulated lymphocyte proliferative responses restraint stress for either a min or h duration had no effect on lymphocyte responses SE to a combination of restraint stress and acute morphine mgkg resulted in a suppression of lymphocyte responses which was similar in magnitude to that observed with morphine administration alone when rats were injected twice daily for days with increasing doses of morphine ranging from mgkg to mgkg morphine mgkg administration on day was not accompanied by either analgesia or depressed blood lymphocyte proliferative responses these results indicated that tolerance had developed to both the analgesic and the IS effects of morphine however upon SE of morphinetolerant animals to restraint AS significant analgesic responses were retained furthermore in contrast to the lack of suppression following restraint AS on lymphocyte responses in salineinjected animals restraint for min produced greater than a suppression in morphinetolerant animals these data suggest that morphine tolerance may be accompanied by an enhanced susceptibility to the IS effects of stress
|
MEDAL
|
prolidase deficiency is a rare hereditary disorder with a wide spectrum of clinical manifestations including skin ulcers eczematous eruptions characteristic facies mental retardation splenomegaly and susceptibility to infections we report two new cases of PD our patients had the typical manifestations of PD one also had lupus erythematosus prolidase activity was either normal or halfnormal in all family members the skin disease in our patients did not respond to topical glycineproline ointment or to oral VC
|
MEDAL
|
correlations between cardiovascular effects of a quantified dose of nicotine and personality measures previously shown to predict coronary heart disease were obtained thirty male sm smoked a popular brand of a regular strength cigarette mg ftcestimated nicotine delivery on one occasion and a nicotinefree cigarette on another occasion by means of a quantified smoke delivery system partial correlations controlling for effects of body weight questionnaireassessed nicotine tolerance and cardiovascular responses to the nicotinefree control cigarette showed jenkins activity survey type a scores to correlate positively with nicotineinduced increase in diastolic blood pressure but negatively with nicotineinduced increase in systolic blood pressure partial correlations indicated that trait anxiety and depression were significantly associated with nicotineinduced HR rate increases but not with nicotineinduced blood pressure responses
|
MEDAL
|
acute myeloid leukemia aml is one of the most common types of leukemia in adults but there is limited information on survivors quality of life qol T3 remission
|
MEDAL
|
responses of oncenter starburst amacrine cells to steady CS stimuli were recorded in the darkadapted mouse retina the response to spots of dim WG CS appear to show two components an initial peak that correspond to the ON of the CS CS- and a series of oscillations that ride on top of the initial peak EC50 the frequency of oscillations during light stimulation was three time higher than the frequency of spontaneous oscillations recorded in the dark the lightevoked responses in starburst cells were exclusively dependent on the PR of glutamate likely from presynaptic BP axon terminals and the binding of glu to ampakainate receptors because they were blocked by cyanonitroquinoxalenedione the synaptic pathway responsible for the light responses was blocked by ap an agonist of metabotropic glu receptors that hyperpolarize oncenter BP cells on activation CS responses were inhibited by the calcium channel blockers cadmium ions and nifedipine suggesting that the release of glu was calcium dependent the oscillatory component of the response was specifically inhibited by blocking the glu transporter with dthreobetabenzyloxyaspartic acid suggesting that glutamate reuptake is necessary for the oscillatory release gabaergic antagonists BIC sr and PT increased the amplitude of the initial peak while they inhibit the frequency of oscillations ttx had a similar effect strychnine the blocker of glycine receptors did not affect the initial peak but strongly decreased the oscillations frequency these GABA inputs onto the bipolar axon terminals shape and synchronize the oscillatory component
|
MEDAL
|
the present T0 was undertaken to assess the effects of the natural antioxidant anthocyanins on learning and memory of rats in exp model of oxidative stress our preliminary experiments demonstrated that disruption of diurnal rhythm via exposition of rats to constant CS for days caused excessive generation of FRs in their brains it is known that free radicals impair cognitive functions this T0 investigated the effects of anthocyanins on cognitive functions of rats in a shuttlebox active avoidance test in the shuttlebox stressed rats showed significantly increased RL time and decreased number of avoidances and escapes in the learning sessions rats treated with anthocyanins had increased number of avoidances and escapes and significantly decreased latency time during the learning sessions our results demonstrated that this model of oxidative AS impaired learning and SM of experimental rats moreover chronic administration of anthocyanins mgkg orally improved BB functions of the rats our data suggest that anthocyanins have a protective role on rat brain and improve cognitive functions in this model of oxidative AS
|
MEDAL
|
here we report the CS of the region that determines rapid allograft rejection in chickens the chicken major DLA mhc this kilobase region of the b locus contains only genes making the chicken mhc roughly fold smaller than the human mhc virtually all the genes have counterparts in the HLA defining a minimal essential set of mhc genes conserved over million years of divergence between birds and mammals they are organized differently with the class iii region genes located outside the class ii and class i region genes the absence of proteasome genes is unexpected and might explain unusual peptidebinding specificities of chicken class i molecules the presence of putative natural killer receptor genes is unprecedented and might explain the importance of the b locus in the response to the herpes virus responsible for mareks diseases the small size and simplicity of the chicken mhc allows coevolution of genes as haplotypes over considerable periods of time and makes it possible to T0 the striking mhcdetermined pathogenspecific disease resistance at the molecular level
|
MEDAL
|
this T0 evaluated the role of stressinduced reductions in FI in Pr bdf mice in the production of developmental abnormalities in the F1 one group of dams underwent one hour of PCS restraint AS twice daily from days to of gestation a second group was not restrained but during this period each dam was pairfed to a weightmatched partner in the stressed group a third group was left undisturbed and allowed unlimited access to LC and water the restraint AS procedure reduced the average daily food and water intake in the dams resulting in lower maternal weights with the unstressed pairfed group being affected more severely than the stressed group the offspring of both the stressed and pairfed dams were lighter than the F1 of the dams fed AL on day postconception and exhibited lower brain weights on day PC neurobehavioral OD as assessed on a battery of sensorimotor tests on day PC was retarded in the prenatally stressed pups in comparison to the AL pups while the pairfed pups did not differ from either group there were no differences among any of the CG on day body or BB weight body or tail length AGD or locomotion rearing and defecation in the open fieldabstract truncated at words
|
MEDAL
|
squarewave anodicstripping voltammetry swasv was set up and optimized for simultaneous determination of Cd lead and copper in siliceous spicules of marine sponges directly in the hydrofluoric acid solution approximately mol l hf ph approximately a thin mercuryfilm electrode tmfe plated on to an hfresistant epoxyimpregnated graphite rotatingdisc support was used the optimum experimental conditions evaluated also in terms of the SNR ratio were as follows deposition potential mv vs agagcl kcl mol l deposition time min electrode rotation rpm sw scan from mv to mv sw pulse amplitude mv frequency hz deltaestep mv tstep ms twait ms tdelay ms tmeas ms under these conditions the metal peak potentials were cd mv pb mv cu mv the electrochemical behaviour was reversible for pb quasireversible for cd and kinetically controlled possibly following chemical reaction for cu the linearity of the response with concentration was verified up to approximately microg l for cd and pb and approximately microg l for cu the detection limits were ng l ng l and ng l for cd pb and cu respectively with td min the method was applied for determination of the metals in spicules of two specimens of marine sponges demosponges from the portofino natural reserve ligurian sea italy petrosia ficiformis and terra nova bay ross sea antarctica sphaerotylus antarcticus the metal contents varied from tens of ng g to approximately microg g depending on the metal considered and with significant differences between the two sponge species
|
MEDAL
|
in vivo i.t. of a NT monoclonal antibody ack to the receptor IMT kinase ckit disrupts the normal motility patterns of the mouse small intestine immunohistochemical studies showed that cells expressing ckitlike immunoreactivity ckitli decreased in numbers in response to ack but the identity of these cells is unknown we investigated the identity and OD of the cells that express ckitli in the mouse small intestine and colon cells in the region of the myenteric plexus and DMP of the small intestine and in the subserosa in the MP region within the circular and LM layers and along the submucosal surface of the circular muscle in the colon were labeled with ack the CSD of cells that express ckitli was the same as that of interstitial cells ics in wholemount S9 cells with ckitli were interconnected forming a network similar to the network formed by cells that stained with methylene blue which has been used as a marker for ics in the mouse gastrointestinal tract immunocytochemistry verified that ics were labeled with ack multiple i.v. of animals with ack between days and post partum pp caused a dramatic reduction in the number of ics compared to control animals from an ultrastructural point of view the proliferation and OD appeared to be suppressed in some classes of ics while others displayed an altered course of OD PET studies showed that the decrease in ics was accompanied by a loss of electrical rhythmicity in the small intestine and reduced neural responses in the small bowel and colon morphological experiments showed that ckitpositive cells are ics and physiological evidence reinforced the concept that ics are involved in generation of rhythmicity and translation of neural inputs in gastrointestinal smooth muscles controlling the OD of ics provides a powerful new tool for the investigation of the physiological role of these cells
|
MEDAL
|
mutations in the cystic fibrosis transmembrane conductance regulator gene cftr manifest on the nasal epithelial surface of individuals with cystic fibrosis cf by na hyperabsorption and diminished betaagonistinduced cl conductance resulting in an abnormal bioelectric phenotype across the nasal epithelium a clinical trial was conducted to determine if a replicationdeficient recombinant adenovirus vector containing a normal copy of the cftr cdna adcftr could when administered to the nasal epithelium correct the abnormal bioelectric phenotype nine individuals with cf received x to x PI forming units of adcftr to the epithelium of one nostril measurements made included baseline electrical potential difference pd between the surface of the nasal epithelium and the ISF change in pd in response to amiloride which inhibits apical na channels and change in pd in response to isoproterenol in a low cl solution a measure of campregulated cl conductance the functional integrity of the epithelium was evaluated by the pd response to atp each individual served as their own control with measurements made in the nostril to be treated before adcftr administration and in the contralateral untreated nostril on the average in the treated nostril over weeks T3 the local administration of the adenovirus vector compared to measurements made in the same nostril before treatment baseline pd decreased toward normal to p response to amiloride decreased toward normal to p and response to low cl and isoproterenol increased toward normal to p there were no changes in response to atp to p suggesting that the epithelium remained functionally IN importantly there were no significant changes in measurements made in the untreated nostril while limited to the nasal epithelium these data suggest an adenovirus vector can safely deliver sufficient cftr cdna function to improve the abnormal cf bioelectric phenotype
|
MEDAL
|
the aim of this pilot study was to evaluate the clinical tau of mineral trioxide aggregate mta as an apexification material when used in nonvital immature permanent incisors in children fifteen children with a mean age of years and nonvital permanent incisors were ajudged suitable for inclusion standard endodontic procedures were followed and an apical plug of mm was created by using mta T3 a calcium XTT intracanal dressing had been applied for at least week final obturation was completed by using thermoplastisized GP obtura ii at least week following mta placement subjects were reviewed clinically and radiographically at month intervals mean followup time for mta was months sd of the total of teeth treated mta placement was considered to be adequate in teeth the procedure showed clinical success in of the cases radiographic success was found to be and in further three cases the outcome was considered to be uncertain this is one of the very few studies that have reported the out coming of mta as an apexification material in children with nonvital teeth and incomplete root OD however larger clinical studies are required to evaluate the LT success of this procedure
|
MEDAL
|
despite adjuvant radiotherapy survival outcomes remain poor in patients with salivary gland HM who have multiple poor prognostic factors this study aimed to determine which patients may benefit from treatment intensification
|
MEDAL
|
hypertensive disorders of pregnancy are the second leading cause of maternal deaths worldwide superimposed preeclampsia is an increasingly common problem and often associated with impaired placental perfusion understanding the underlying mechanisms and developing treatment options are crucial the pregnant SHRSP rat has impaired uteroplacental blood flow and abnormal uterine i.a. CR we used ang ii angiotensin ii infusion in Pr strokeprone SH rats to mimic the increased cardiovascular stress associated with superimposed preeclampsia and examine the impact on the maternal CVS and fetal development continuous infusion of ang ii at or ngkg per minute was po from gestational day until term radiotelemetry and echocardiography were used to monitor hemodynamic and cardiovascular changes and urine was collected prepregnancy and throughout gestation uterine i.a. myography assessed uteroplacental V1 function and structure fetal measurements were made at gestational day and placentas were collected for histological and mRNA analyses the ngkg per minute ang ii treatment significantly increased blood pressure
|
MEDAL
|
we experienced a case of advanced maternal age in which a fetus was found to be positive for trisomy at reexamination following indeterminate noninvasive prenatal genetic testing nipt the amniotic fluid chromosomal test revealed a normal karyotype and confined placental mosaicism cpm was observed in an snp microarray analysis of the placenta the child was born with no defects or complications in the present case the result of the original nipt at week of pregnancy was ind and the subsequent reexamination result was positive since the definitive normal diagnosis was not reported until the latter half of week the Pr patient was subjected to psychological stress for a long period of time the problem with nipt is that most of the fetusderived CF dna in the maternal blood is not derived directly from the fetus but from the villus cells of the placenta leading to indefinite diagnoses for that reason the pregnant patient was subjected to psychological AS for a long period of time of the cases undergoing nipt in the past years in japan had indeterminate results this was the second case in which a subsequent reexamination gave a positive result for trisomy
|
MEDAL
|
we examined the role of ouabainlike compound olc in hypertension associated with ACTH acth excess in rats physiological saline solution mlkg or synthetic acthz mgkg was injected intramuscularly for days to control and male WR significant increases in blood pressure and plasma sodiumpotassium ratio and decreases in PK concentration and urinary sodiumpotassium ratio were observed in acthtreated rats the plasma olc level was higher in acthtreated group control acth pmoll p plasma olc level correlated with systolic blood pressure spb r p urinary olc excretion was also higher in acthtreated group control acth pmolday p a significant relation was also found between urinary olc excretion and sbp r p PK negatively correlated with sbp r p and urinary sodiumpotassium ratio also correlated inversely with urinary olc excretion r p measurement of olc C2 after the fractionation of urine by reversephase high performance liquid chromatography showed that the L1 olc peak in urine from both groups coincided with that of authentic ouabain these results suggest the contribution of olc to acthinduced hypertension in rats
|
MEDAL
|
the id inhibitors of dna bindingdifferentiation proteins represent a family of dominant negative regulators of the basic HLH transcription factors whose activities result in delayed cell differentiation and prolonged proliferation a pair of expressed sequence tag clones with homologies to the id proteins were identified and used to screen a rainbow trout bacterial artificial chromosome bac library to identify clones containing homologues sequences phylogenetic analysis of the predicted amino acid sequences revealed close similarities to the rainbow trout id protein the genes were therefore classified as rainbow trout idb and idc genome characterization based on bac sequencing showed each gene to have two exons separated by a small intron the genes are similar in their transcribed regions yet they are only and similar in the upstream and downstream sequences respectively using reverse transcription polymerase chain reaction we found both genes to be expressed in a variety of tissues in the AD rainbow trout
|
MEDAL
|
the study of the nucleoli in the lymphocytes of human IP blood and articular exudations revealed that the lymphocytes in inflammatory articular exudbnormal csf neurologic PS antedated the appearance of the pulmonary infiltrate and have persisted despite appropriate antibiotic therapy a review of the literature revealed only one other similar case legionnaires disease should be considered in the differential diagnosis of encephalomyelitis even in the absence of a pneumonic infiltrate
|
MEDAL
|
to elucidate the mechanisms by which HPCs transmigrate via the bone marrow bm EC cells we first established EC cell lines from bm and lung and bm fibroblast cell lines then we established an in vitro MM of transendothelial migration of HPCs in the presence of chemoattractants secreted by bm FB cells the bm endothelial cells expressed vascular cell adhesion molecule vcam but the lung EC cells did not the bm fibroblast cells secreted chemoattractants including stroma cellderived factor sdf which could attract hematopoietic progenitor cells to bm and activate the adhesion molecules expressed on hematopoietic progenitor cells T3 rolling along the endothelial cells antisdf antibody inhibited the TEM of a hematopoietic progenitor cell line fdcp fdcp that expressed very VLA antigen vla and normal progenitor cells transmigrated through bm EC cells but not lung EC cells even if in the presence of chemoattractants produced by bm fibroblasts both antivla and antivcam antibodies inhibited the transendothelial migration of fdcp cells and normal hematopoietic progenitor cells these findings suggest that the transendothelial migration of hematopoietic progenitor cells is characteristic of bm EC cells and that vlavcam and sdf play important roles in the transendothelial migration and consequently homing of hematopoietic progenitor cells to bm
|
MEDAL
|
recombinant murine gamma interferon as well as lymphokines prepared from immune splenocytes and concanavalin astimulated tcell hybridoma activated normal mouse peritoneal macrophages to inhibit the intracellular growth of Hc the MICs of the lymphokine from immune splenocytes and of rMuIFN-gamma were neutralized by rabbit antimurine gamma interferon antibody the intracellular yeasts were not killed by the interaction even though growth was CR inhibited
|
MEDAL
|
to evaluate the efficacy of tensionfree vaginal tape tvt compared with other surgical treatments for AS UI sui and with other tensionfree midurethral slings
|
MEDAL
|
Subsets and Splits
No community queries yet
The top public SQL queries from the community will appear here once available.