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the purpose was to investigate the effect of weeks heavy ST in young elite cyclists nine cyclists performed endurance training and heavy ST es while seven cyclists performed ET only e es but not e resulted in increases in isometric half squat performance lean lower body mass PPO during wingate test peak aerobic power SO wmax SP SO at mmol lla mean power SO during min allout trial and earlier occurrence of PT during the pedal stroke p es achieved superior improvements in wmax and mean power SO during min allout trial compared with e p the improvement in min allout performance was associated with the change toward achieving PT earlier in the pedal stroke r p neither of the CG displayed alterations in vomax or cycling economy in conclusion heavy strength training leads to improved cycling performance in elite cyclists as evidenced by a superior effect size of es training vs e training on relative improvements in power output at mmol lla peak power output during s wingate test wmax and mean power SO during min allout trial
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the aim of this work was to perform a comparative investigation of EA aggregability changes in the peripheral and CBF circulation during ischemic and hemorrhagic stroke subjects of the present T0 were patients with ischemic brain infarcts patients and with hemorrhagic stroke patients from the intensive care unit of the institute of neurology and neurosurgery the blood samples were obtained from the following blood vessels the common i.c. carrying blood to the primarily damaged brain hemisphere both jugular veins carrying blood from the primarily damaged and the contralateral hemispheres as well as from the cubital vein to obtain specimens of the systemically circulating blood erythrocyte aggregation was evaluated by using the georgian technique we found that the rbc aggregation indices increased in both the regional as well as the SVR circulation of the hemorrhagic stroke patients as compared to ischemic stroke patients the results of the present study demonstrate different changes of erythrocyte aggregation in ischemic and HS patients therefore the role of blood rheological properties in their pathogenesis seems also to be different
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acquired immune deficiency syndrome aids continues to increase in the united states especially among minority CG the disease is impacting not only individuals but entire families through multiple infections of family members this results in simultaneous illness and multiple loss within the family system delivery and management of quality care for the family is often made difficult by the lack of resources experienced by families with limited income and the multiplicity of problems associated with poverty in the us this presents numerous challenges to HCPs utilizing a case T0 this article presents a model demonstrating the coordination of services among several providers as a means of meeting a variety of family needs and providing SQ costeffective care
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cyst fluids from mucinous cystadenocarcinoma of the ovary show significant concentrations of carcinoembryonic antigen cea activity this cea activity was compared to several colon cea standards with respect to size concanavalin a binding and immunological activity cea activity in unfractionated ovarian cyst fluid was indistinguishable from cea standards by GF chromatography and sodium dodecyl sulfatepolyacrylamide gel PACE the cyst fluid cea activity showed identity with various cea standards by double immunodiffusion in agar gels and by radioimmune competition assay attempts to purify the cyst fluid cea by PCA SE prior to GFC were unsuccessful the yield of soluble cea activity following PCA SE averaged and in one cyst fluid qualitative changes were noted in the soluble antigenic activity ovarian cyst fluid cea activity was bound by concanavalin asepharose b and specifically eluted with competing monosaccharide lectin affinity chromatography and gel filtration chromatography over sepharose b and sephadex g can be used to purify cyst fluid cea from whole fluid or lyophilized and reconstituted samples
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to investigate whether skinsparing mastectomy ssm and immediate breast reconstruction ibr is a possible treatment option in selected cases of locally recurrent breast CA after previous BCT bct
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a case of multiple supratentorial hemangioblastomas combined with a primary cerebellar manifestation is reviewed there was no association with polycythemia or von hippellindau syndrome the authors emphasize neuropathological and neuroradiological methods in detecting this rare disease
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this article examines the impact of the medicare prospective payment system pps on the tages especially when the tumor gains substantial size weight of several pounds at the time of diagnosis because it is grows very silently in deep tissues in the retroperitoneal area therefore most of the patients with liposarcoma have no symptoms until the tumor is getting very large and pressurizes on neighboring structures which causes tenderness pain or functional disturbances a yearold male patient admitted with oneyear PH of ABD pain distention computed tomography demonstrated a C1 RP mass in fat density filling the pelvic cavity extending to epigastric region especially in the left side of abdomen and displacing intestines to the right and left kidney and pancreas gland posteriorly at LAP the retroperitoneal RT weighed kg histologically it was a welldifferentiated liposarcoma total extirpation surgery is still the most effective treatment in welldifferentiated liposarcomas close followup T3 surgery is mandotary due to high rates of recurrence fig ref full text free pdf wwwbmjsk
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CSD is a propagating wave of depolarization that plays important roles in migraine stroke subarachnoid haemorrhage and brain injury cortical spreading depression is associated with profound vascular changes that may be a significant factor in the clinical response to CSD events we used a combination of optical intrinsic signal imaging electrophysiology potassium CS electrodes and spectroscopy to investigate neurovascular changes associated with cortical spreading depression in the mouse we identified two distinct phases of altered neurovascular function one during the propagating cortical spreading depression wave and a second much longer phase after passage of the wave the direct current shift associated with the CSD wave was accompanied by marked arterial constriction and desaturation of cortical haemoglobin after recovery from the initial CSD wave we observed a second phase of prolonged negative direct current shift arterial constriction and haemoglobin desaturation lasting at least an hour persistent disruption of neurovascular coupling was demonstrated by a loss of coherence between electrophysiological activity and perfusion extracellular potassium concentration increased during the CSD wave but recovered and remained at baseline after passage of the wave consistent with different mechanisms underlying the first and second phases of neurovascular dysfunction these findings indicate that cortical SD is associated with a multiphasic alteration in neurovascular CF including a novel second direct current shift accompanied by arterial constriction and decrease in tissue oxygen supply that is temporally and mechanistically distinct from the initial propagated cortical SD wave vascularmetabolic uncoupling with CSD may have important clinical consequences and the different phases of dysfunction may represent separate therapeutic targets in the disorders where cortical spreading depression occurs
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the PD abnormalities in kawasaki disease kd often cause MI previous publications have described the use of thallium MPI to determine the extent of ischemia in patients with kd the technetium perfusion agents offer better resolution and may offer additional information about VF in these patients this study was performed to evaluate myocardial perfusion in patients with kd through use of Tc m TF tf in conjunction with dipyridamole vasodilator stress
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a pcrbased method was developed for the detection and ID of two species of grasshopperspecific pathogens belonging to the genus entomophaga in north america entomophaga calopteni and entomophaga macleodii two separate sets of primers TPS for amplification of a dna product from each species of entomophaga as well as a positive control were utilized grasshoppers were collected from two CS in mexico during an epizootic with grasshoppers found in summit disease typical of entomophaga infections there was a preponderance of melanopline grasshoppers infected by e calopteni the described method is an accurate tool for ID of north american grasshopper infections by entomophaga species
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a hexanucleotide ggggcc repeat expansion in corf is the most common genetic cause of ALS and frontotemporal degeneration accurate determination and quantitation of the repeat length is critical in both clinical and research settings however because of the complexity of the corf expansion with high gc content large size of repeats and high rate of insertionsdeletions indels and CS variations in the flanking regions molecular genetic analysis of the locus is challenging to improve the performance characteristics for clinical testing we evaluated a commercially available longread corf pcr assay for research use only amplidex pcrce corf CA amplidexc and compared its performance with our existing laboratorydeveloped corf expansion procedure overall in comparison to the laboratorydeveloped corf expansion procedure amplidexc demonstrated a more efficient workflow greater pcr efficiency for sizing of repeat expansions and improved peak amplitude with lower dna input and higher analytic sensitivity this in turn permitted PCD of indels in the downstream of the repeat expansion region in expanded alleles showed a higher success rate with formalinfixed PET specimens and facilitated the assessment of repeat mosaicism in summary amplidexc will not only help to improve clinical testing for corfassociated amyotrophic lateral sclerosis and frontotemporal degeneration but will also be a valuable research tool to better characterize the complexity of expansions and T0 the effects of indelssequence variations in the flanking region
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the objective is to consider the problem of HBP bp a leading global risk factor associated with substantial morbidity and mortality despite the availability of treatment guidelines and a wide range of therapies bp control is suboptimal in many countries recent data indicate that only around of patients manage to achieve an adequate level of bp control a group of international experts in the field of hypertension met in to consider this problem the resulting white paper delivered an urgent call to action and identified six key issues for improving bp control in a working group of investigators spontaneously undertook an action with the primary aim of considering the current hypertension management situation in europe to discuss whether the situation had changed since and to determine what can be learnt from the projects in other continents such as the canadian hypertension education program which has shown that higher C2 of bp control can be achieved across a GP the working group identified the main challenges affecting the improvement of bp control today and suggests five key actions identify the bp treatment target of less than mmhg for the majority of patients simplify treatment strategies and encourage pill reduction decrease therapeutic inertia improve patient empowerment and involve healthcare systems and reduce the prevailing focus on drug costs in many healthcare systems implementing key actions identified by the WG may help to improve achievement of better bp control across europe
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sufficient trauma to the chest can result in injury to the bony thorax and soft tissues of the chest wall increasing patient morbidity and mortality fractured ribs can lacerate the pleura lung or ABD organs fractures to upper ribs clavicle and upper sternum can signal BP or V1 injury paradoxical movement of a flail chest can impair respiratory mechanics promote atelectasis and impair pulmonary drainage most patients with thoracic spine fracturedislocations have complete neurologic deficits scapular fractures associated with other injuries in almost all patients are frequently overlooked on supine chest radiographs sternal fractures associated with clinically silent myocardial contusion are best visualized on chest computed tomography ct severe trauma to the CW can be associated with large chest wall hematomas or collections of air within the CW that can communicate with the i.t. space ct scanning can easily distinguish chest wall from parenchymal or mediastinal injury whereas this differentiation my not be possible with chest radiography
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pf is a novel biological agent for CHC virus hcv therapy it comprises a recombinant adenoassociated virus aav dna vector packaged into an aav serotype CA the vector directs expression of three short hairpin rnas shrnas targeted to conserved regions of the hcv genome these shrnas are processed by the host cell into the si rnas which mediate sequencespecific cleavage of target regions for smallmolecule inhibitors the key screens needed to assess in vitro activity are well defined we developed new assays to assess this rna interference agent and so to understand its therapeutic potential following administration of pf or corresponding synthetic shrnas sequencespecific antiviral activity was observed in hcv replicon and infectious virus systems to quantify the numbers of shrna molecules required for antiviral activity in vitro and potentially also in vivo a universal QPCR qpcr CA was developed the number of shrna molecules needed to drive antiviral activity proved to be independent of the vector delivery system used for pf administration the emergence of resistant SCV at the target site of one shrna was characterized a novel rna cleavage assay was developed to confirm the spectrum of activity of pf against common hcv clinical isolates in summary our data both support antiviral activity consistent with an rna interference mechanism and demonstrate the potential of pf as a therapeutic agent for chronic hcv infection
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insulin resistance in viral infections is common we have explored the effectiveness of metformin for alleviati an G1 sludge reactor was operated at three different srts and days submerged membrane experiments were performed when the mixed liquor suspended solids mlss concentration reached the steady state conditions mlss concentrations reached the steady state at ± ± and ± mgl for srts of and days respectively the total soluble microbial products smp were ± ± and ± mgl at srts of and days respectively the carbohydrate concentration in the supernatant was about two times more for srt of days than that for days the total amount of extracellular polymeric substances eps extracted from the flocs were approximately ± ± and ± mgg mlss at three srts and days under the same organic loading rate the viscosity of the biomass increased with the increasing srt the results of flux stepping tests showed that the membrane fouling at srt days was always higher than that of and days four different MF membranes cellulose acetate polyethersulfone mixed ester and PC with three different pore sizes μm were tested filtration resistances were determined for each membrane cake resistance was observed to be the most significant fouling mechanism for all membranes
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ultrasonography us of the thyroid gland has recently proved to be a useful clinical diagnostic method and the newly developed highresolution us with a color doppler CFM CF can reveal fine details of the TG and the hemodynamic features of a thyroid neoplasm us can yield a diagnostic accuracy of more than for thyroid carcinoma especially papillary carcinoma however neither conventional bmode us imaging nor ABC has delivered satisfactory results for follicular carcinoma the aim of this study was to evaluate the clinical usefulness of CD imaging for the differential diagnosis of thyroid follicular lesions a color doppler scan was performed in patients with a solitary cold nodule and a combination of bmode us and color doppler findings including RT vascularity and results of a fast fourier transform fft analysis were used to create the following diagnostic grading system for differential diagnosis of follicular lesions grade benign follicular lesion no color CFM cfm inside the nodule grade benign IP type cfm only in peripheral area pulsatility index pi grade suspected follicular carcinoma penetrating cfm vascularity moderate grade follicular carcinoma highvelocity penetrating cfm pi all patients were subjected to surgical resection and histologic examination was used to confirm the diagnosis the grades of the adenomatous nodules were as follows grade grade grade grade the corresponding percentages for the follicular adenomas were and and for the follicular carcinomas they were and on the assumption that grade and lesions are benign and grade and lesions are malignant of the follicular carcinomas and of the benign tumors had been accurately diagnosed yielding a sensitivity of a specificity of and an accuracy of for the grading system CDI of follicular tumors has revealed that highvelocity pulsatile blood flow penetrating the RT is a characteristic finding of follicular carcinoma and is a new diagnostic criterion for performing color doppler imaging the differential diagnostic grading scores for CD examinations and the results of fft analysis demonstrate that us with the color doppler CF can play an important role in the differential diagnosis of thyroid PT
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human BB tissue is a valuable source of material for research it is often stored indefinitely in formalin at RT which may weaken the immunolabeling with formalinsensitive antibodies the present study found that a novel protocol that combined citrate and formic acid pretreatments with the catalyzed signal amplification csa system was able to recover the lost or weakened immunolabeling with the formalinsensitive antibodies anticd anticaveolin P-gp antineuronal nuclei antiparvalbumin antihuman leukocyte antigen anticd anticd and anticonnexin in postmortem human BB tissue that was stored in formalin for up to years at RT recovered immunolabeling in longfixed tissue resembled immunolabeling observed in tissue that was fixed for a shorter duration between and days the findings from this T0 highlight the importance of testing antibodies for formalin fixation effect prior to studies especially if longfixed tissue is used to enable immunolabeling to be more accurately interpreted importantly this T0 provides a method of overcoming formalinmasking of antigens in longfixed human tissue thus allowing essential immunohistochemical studies to be undertaken using precious human tissue
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this review presents information about carbonic anhydrases enzymes catalyzing the reversible hydration of carbon dioxide in AQ solutions the families of carbonic anhydrases are described and data concerning the presence of their representatives in organisms of different classes and especially in the higher plants are considered proven and hypothetical functions of CAs in living organisms are listed particular attention is given to those functions of the enzyme that are relevant to photosynthetic reactions these functions in algae are briefly described data about probable functions of carbonic anhydrases in plasma membrane mitochondria and chloroplast stroma of higher plants are discussed update concerning CAs in cp thylakoids of higher plants ie their quantity and possible participation in photosynthetic reactions is given in detail
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this study on a microcosm brings out the temporal changes in physicochemical behavior of Al2O3 NPs for a period of days at environmentally relevant concentrations μgl and below the dynamics of particle SMB in terms of mean hydrodynamic diameter specific surface area and dissolution of soluble aluminum and their possible ecological implications have been presented in this T0 a thorough statistical analysis brings out nanoparticle SMB where a rapid aggregation of particles ± nm at h to ± nm at h with a decrease in SSA mg at h to mg at h was observed ion PR profile indicated a significant increase in soluble aluminum concentration only after h ± μgl at h to ± μgl at h which reduced over a period of days ± μgl a differential response at μgl concentration was observed short term SE days showed an immediate effect on the resident algal population ⼠decreased viability and the long term months days exposure showed a gradual REC thus nanomaterials may not have the stipulated toxic response at low concentration and longer standing period presumably owing to the complexity of the NK systems
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tetrahydrolipstatin thl derived by hydrogenation from lipstatin a lipase inhibitor produced by streptomyces toxytricini has been shown to inhibit in vitro the activity of all three lipases secreted to the gastrointestinal tract gastric lipase pancreatic lipase and carboxylester lipase cholesterol ester hydrolase the effects of thl on intestinal absorption of fat transport to the thoracic duct chyle has now been investigated after intraduodenal infusion in a rat model absorption of label from OA when administered with monoolein in micellar bile salt solution was not affected by thl in concentrations up to m c values of HW to body weight ratio in isolated perfusion the PET capacities of NF from isotreated rats were severely depressed compared to normal rat NF whereas exercisetrained rat NF performed as well or even better than control hearts the functional REC of isotreated hearts following cardioplegiainduced arrest for min at degrees c was significantly worse than the recovery of normal NF but hearts of exercisetrained rats showed a significantly better REC than control NF exercise training results in improvement of myocardial blood supply resulting in better preservation of the heart during ischemia compared to normal hearts addition of a combination of verapamil and diltiazem to the CPS followed by ICM arrest for min at degrees c resulted for isotreated rat hearts in an improved recovery of cardiac output compared to cardioplegia in the absence of these drugs in exercisetrained and control rat NF calcium antagonists improved the REC from cardioplegic arrest of cardiac output from to and from to respectivelyabstract truncated at words
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bacterial agents are considered important pathogens causing external otitis in dogs it is essential to carry out bacterial SC and antimicrobial susceptibility test in the case of OE particularly for chronic or recurring cases sterile swab samples were obtained from terminal part of vertical ear canals of dogs with OE for cytology bacterial SC and antimicrobial susceptibility test cytologic smears were stained using gram and giemsa staining methods aerobic bacterial culture performed on blood agar and macconkey agar among total number of isolated bacteria were staphylococcus intermedius other isolated bacteria included pseudomonas aeruginosa proteus mirabilis escherichia coli pasteurella canis and six other species of coagulasenegative staphylococcus antimicrobial susceptibility test were performed for all isolated bacteria using antibiotics based on the results of this T0 all isolated staphylococcus spp were sensitive to amikacin enrofloxacin and RFP and had low resistance to gentamicin cephalothin and ceftriaxone more than half of grampositive isolates were resistant to penicillin and ampicillin generally all isolated GNB were sensitive to amikacin and ENR and had low resistance to ceftriaxone and gentamicin they were highly resistant to penicillin eythromycin and CET regarding the results of this study in cases of uncomplicated otitis externa it is possible to select antimicrobial drugs merely based on cytology but it is recommended to perform bacterial SC and antimicrobial susceptibility test however in complicated or refractory cases antimicrobials should be selected based on bacterial culture and antimicrobial susceptibility test
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the effect of phorbol myristate acetate pma on hepatocyte alpha adrenergic receptors was determined by hprazosin binding to PMs from control and pmatreated hepatocytes CM from HCs incubated with pma microgramml for h exhibited a decrease in alpha adrenergic receptors fmolmg of protein mean sem for three separate experiments relative to vehicletreated DMF HCs fmolmg of protein n with no significant effect on the kd the pmainduced decrease in alpha adrenergic receptors was maximal by min and halfmaximal inhibition of hprazosin IB occurred with a pma concentration of approx ngml i.c.v. of hepatocytes with staurosporine microm blocked the effect of pma and betaphorbol monoacetate was ineffective suggesting the involvement of protein kinase c pkc treatment of HCs with primaquine microm for min decreased hepatocyte BPM alpha adrenergic receptors by mean sem of three experiments removal of primaquine allowed essentially complete recovery mean sem for five separate experiments of plasma membrane hprazosin IB within min suggesting that the alpha adr undergoes endocytotic recycling addition of pma microgramml to hepatocytes immediately after removal of PQ CR inhibited the increase in BPM alpha adrenergic receptors relative to control cells but had no effect on HCs whose cell surface alpha receptors remaining T3 primaquine treatment had been inactivated by alkylation these observations suggested that activation of pkc may facilitate the internalization of the alpha adrenergic receptor in HCs
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coke oven workers are exposed to high levels of carcinogenic polycyclic aromatic HCs including benzoapyrene bap and are at increased risk of LC since bap is enzymatically activated to beta alphadihydroxy alpha alphaepoxytetrahydrobenzoapyrene bapde that forms adducts with dna the presence of these adducts was measured in dna from peripheral blood lymphocytes by synchronous fluorescence spectrophotometry and enzyme radioimmunoassay approximately twothirds of the workers had detectable C2 of bapdedna adducts antibodies to the dna adducts were also found in the SS of of the workers bapdedna adducts were not detectable in lymphocytes and antibodies to the adducts were not detected in sera from a control group of nonsmoking laboratory workers dna adducts andor antibodies to the adducts iroencephalographic activity in the frontopolar region the intracranial location of the slow electrical activity was estimated as an equivalent current dipole by using a newly developed DT dt method the dt analysis showed that the dipole equivalent of the slow wave is approximately located at the FC part of the left cingulate gyrus away from the margins of the infarction and enlarged left lateral IVT demonstrated by mri and in a region with IN oxygen consumption rate the genesis of the slow wave is discussed
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we use TR photoluminescence from single inp nanowires containing both wurtzite wz and zincblende zb crystalline phases to measure the carrier dynamics of quantum confined excitons in a typeii homostructure the observed recombination lifetime increases by nearly orders of magnitude from ps for excitons above the conduction and valence band barriers to more than ps for electrons and holes that are strongly confined in quantum wells defined by monolayerscale zb sections in a predominantly wz nanowire a simple computational model guided by detailed highresolution transmission electron microscopy measurements from a single nanowire demonstrates that the dynamics are consistent with the calculated distribution of confined states for the electrons and holes
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infection of vero cells with african swine fever virus asfv resulted in a marked increase in ribonucleotide reductase activity the induction of ribonucleotide reductase was detected early T3 infection and was proportional to the multiplicity of infection inhibition of viral dna replication did not affect the induction of the enzyme several characteristics could distinguish the virusinduced from the normal cell enzyme asfvinduced ribonucleotide reductase was inhibited by magnesium was more strongly inhibited by hydroxyurea and had a fourfold lower km the virusinduced enzyme was inhibited by deoxyribonucleoside triphosphates and by atp the isolation of hydroxyurearesistant asfv mutants provided genetic evidence for the viral origin of the induced ribonucleotide reductase the resistance to hydroxyurea was due to a threefold overproduction of ribonucleotide reductase as compared to enzyme induction by wildtype asfv HU had similar effect in vitro on ribonucleotide reductases induced by wildtype or mutant virus the gene for the small S1 of the viral enzyme was mapped within a kb fragment by hybridization with an oligonucleotide probe designed from a conserved AA sequence of eukaryotic and viral ribonucleotide reductases
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to examine SQ of life and coping strategies among immigrant women living with chronic pain
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glaucomatous damage is a neurodegenerative eye disease and one of the leading causes of blindness with million patients worldwide L1 currently challenging questions include early diagnosis risk DUE and followup circulating WBCs have been demonstrated as potentially important source of disease TPS markers the relevance of expression alterations in leukocytes for glaucomatous damage needs to be clarified noteworthy gene expression patterns of trabecular meshwork and SC which are anatomically and functionally highly relevant for glaucoma pathology were shown to be similar to those of circulating leukocytes here we report extensive alterations in characteristic protein expression patterns of circulating leukocytes for normaltension and primary openangle glaucoma as revealed by analysis of d page images among most conservative alterations we found the protein spot identified by malditof as basic transcription factor activating proteinbeta apβ westernblot analysis demonstrated significantly increased protein expression rates of apβ in both normaltension and primary openangle glaucoma versus controls ap proteins are essential factors of the basic transcription regulation ap proteins play a decisive role particularly in morphogenesis of eye conservative ap upregulation is of special importance in terms of basic transcriptional dysregulation that might be TPS for glaucoma disease
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to identify hogwash oil quickly the characteristic delta lambda of hogwash oil was analyzed by three dimensional fluorescence spectroscopy with parallel factor analysis and the MM was built up by using synchronous fluorescence spectroscopy with support vector machines svm the results showed that the characteristic delta lambda of hogwash oil was nm collecting original spectrum of different samples under the condition of characteristic delta lambda nm the best MM was established while principal components were selected from original spectrum and the radial basis CF rbf was used as the kernel function and the optimal penalty factor c and kernel function g were and respectively obtained by the grid searching and fold cross validation the discrimination rate of the MM was for both training sets and prediction sets thus it is quick and accurate to apply synchronous fluorescence spectroscopy to ID of hogwash oil
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during placentation the cytotrophoblast differentiates into the villous cytotrophoblast and the extravillous cytotrophoblast the latter invades the decidualized endometrium glycodelina gda is abundantly synthesized by the decidua but not the trophoblast previous data indicate that gda suppresses the invasion of trophoblast cell lines by downregulating proteinase expression and activitied especially in the migration of the work force in theory all countries are either countries of origin either countries of transit or destination and they are all responsible for the rights of migrant workers by promoting the rights by monitoring and by preventing the abusive conditions the process of migration of the workforce can be divided into three stages the first coincides with the period prior to departure the second is represented by the aftermath of the departure and the period of stay in the country of destination the third stage corresponds to the return in the country of origin the workers must be protected throughout this process by the international organizations that perform the catalytic role of communication and exchange between countries for the only purpose of protecting the rights of immigrant andor immigrants workers the responsibility for the protection of workers is divided among the various players in the international labour organisation every country has to apply measures according to the international standards regarding workers rights standards that guide the various countries in the formulation and implementation of their policies and legislation these standards are suggested by international conventions the ilo conventions and other international instruments such as the human rights SF-36 there has been a big step forward once the ilo fundamental conventions and conventions on migrant workers where implemented and this implementation represented the use of the guidelines ilo multilateral FR on labour migration
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the synthesis of specific NHC proteins in the uterus of the ovariectomized rat was examined as a function of time after treatment with E(2) sequential stimulations in the rates of synthesis of at least five nonhistone chromosomal proteins having molecular weights of and respectively were observed the rate of synthesis of the NHC protein having a molecular weight of was increased at hr after hormone treatment this was the first NHC protein to be induced by estrogen and its induction was blocked by pretreatment with actinomycin d the data reported suggest but do not prove that this protein is the s estrogen receptor and that it is the induced nuclear Pa described earlier by teng and hamilton the rates of synthesis of the nonhistone proteins with molecular weights of and were increased at and hr respectively after hormone treatment
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in lymphoedema of the extremities arteriovenous shunt circulation develops regulation of limb circulation was studied in cases of primary and secondary lymphoedema by using the venous ID method and technetiummacroaggregate perfusion scintigraphy as a result of lymphoedema limb circulation total limb circulation increases arteriovenous o difference is low and the VO2 of the tissues is normal the development of arteriovenous micro and macro shunts can be directly visualized by perfusion scintigraphy in lymphoedema of the extremities the nutritivecapillaryblood supply of the tissues remains normal and therefore no trophic disturbances develop lymphoedema is often associated with a fairly considerable AVS circulation and in such cases the cardiac SO as well as cardiac performance are enhanced
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we assess the invivo relationship between international normalized ratio inr and global coagulation tests in patients with lifethreatening bleeding who received prothrombin complex concentrate pcc for warfarin reversal this was a prospective pilot study in AD patients with intracranial bleeding related to anticoagulation with warfarin thromboelastography teg thrombin generation parameters and inr were assessed at baseline min and h T3 pcc changes in laboratory parameters and relationship between inr and global coagulation tests were assessed over time eight patients mean standard deviation sd age were included and received mean sd dose of pcc unitskg four patients died during the study all with inr values more than thirty T2 after pcc mean sd inr was and decreased significantly to thirty minutes after pcc p baseline endogenous thrombin potential and thrombin peak were nmolmin and nmol and increased significantly to nmolmin p and nmol p min after pcc administration reaction rtime decreased significantly p and maximum amplitude and overall coagulation index ci significantly increased during treatment p respectively TG and teg values corrected after pcc administration however inr did not fully correct patients that died tendeng and optimally an observership at an expert centernumerous dedicated esd devices are now available in the united states from different manufacturers although the use of esd in the united states is increasing issues related to technical difficulty limited training opportunities and mentors risk of adverse events long procedure durationand suboptimal reimbursement may limit esd adoption in the united states to a modest number of academic referral centers for the foreseeable future
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recent data have shown that individuals with low insulin sensitivity si also have reduced whole body maximal oxygen uptake the objectives of this study were to determine whether muscle mitochondrial CF was independently related to si T3 being adjusted for known determinants of si and whether lower si among africanamerican aa vs caucasianamerican ca women was due to lower muscle mitochondrial CF among aa women subjects were ca and aa premenopausal women age yr mitochondrial CF time constant of adp adptc was assessed during a s unilateral ISO contraction using p magnetic resonance spectroscopy si with an intravenous gl ATT body composition by dualenergy xray absorptiometry and VAT vat with computed tomography anova was used to compare aa and ca groups and MLR modeling was used to identify independent predictors of si betweenrace comparisons indicated that muscle oxidative capacity was lower among aas vs cas adptc vs s multiple LR models for the dependent variable si contained vat and race and vat race and adptc significant independent effects for all predictor variables were observed in both the first r and second r models the partial correlation for race was lower in the second MM vs suggesting that muscle mitochondrial function contributed to the racial difference in si lower muscle mitochondrial function among aas may in part explain lower si among them
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a complementation system for studying bacteriophage t tail assembly has been developed and used to test the effects of nonviable mutations on the function of a TPS t tail protein gp the complementation system assays the assembly function of gp without requiring that viable phage be produced circumventing the operational problems of maintaining nonviable mutants of this lytic bacteriophage the protein to be tested was preexpressed from cloned genes in a host cell prior to infection with the challenge phage assembly activity was assayed by monitoring the conversion of one tail assembly intermediate the baseplate lacking gp into baseplates containing gp or into tube baseplates or sheathed tails assembled from such baseplates specific incorporation of gp into these structures was confirmed using gpspecific antiserum and the same SS was used in direct immunoelectron microscopy experiments to localize gp to the baseplateproximal end of the t tail tube at the site where the tube and sheath bind to the baseplate the protein gp has been previously shown to be a baseplate protein as well as a tailtubeassociated protein and was tested for a possible role as a taillength tapemeasure protein tests with a deleted variant of gp were inconclusive because the protein was inactive a variant of gp longer than WT due to an internal duplication was found to be partly PET in our assembly complementation system this abnormally elongated protein allows several assembly steps to proceed including the assembly of normal length t tails implying that it does not specify tail length the insertionduplication variant of gp appears to have a defect in its interaction with the tail sheath protein leading to abnormal sheath contraction
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the nonvitamin k antagonist oral anticoagulants noacs exert their anticoagulant effect closely related to their PC since their CSD volume is related to body weight and its correlates ie surface area and body mass index bmi extremes in body weight may affect their tau or safety four noacs are currently available for longterm use with few exceptions in atrial fibrillation and A-V thromboembolism the direct thrombin inhibitor dabigatran etexilate and the factor f xa inhibitors rivaroxaban apixaban and edoxaban experience in patients with low kg or extremely high kg body weight is still quite limited as such patients were rare in registration trials and sometimes directly excluded in general increased bleeding and higher mortality rates are observed in patients weighing kg compared with patients weighing kg this may however also be explained by the presence of underlying conditions such as cancer at the opposite end of the spectrum of body weight lower antithrombotic tau may occur perhaps due to the dilutional effect of a higher distribution volume in this article we review the pertinent literature and analyze the effects of low or high body weight on AC activity and clinical outcomes of the noacs their dose recommendations and areas of uncertainty
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AD can be a lifethreatening problem if not promptly recognized and treated since the most common cause is bladder distension it is essential that the urologist be familiar with this syndrome several patients with serious complications owing to autonomic dysreflexia including mortality are presented the complications of autonomic dysreflexia result from a sudden marked rise in blood pressure which may be severe enough to rupture single or multiple cerebral blood vessels or lead to a considerable increase in intracranial pressure the neurophysiology clinical features and therapeutic measures are discussed
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a novel technique for dissecting a replaced right hepatic artery during pancreatoduodenectomy in patients with middle or lower bile duct carcinoma is presented T3 skeletonizing the left proper common hepatic arteries and the portal vein the replaced RHA is dissected from the ventromedial side of the hepatoduodenal AL by severing the thin connective tissue behind the portal vein thus the hepatic arteries and the portal vein are completely isolated without the kocher maneuver leaving the cancer and the bile duct untouched en bloc with the surrounding lymph NO and the pancreas head the periaortic CL nodes can also be resected en bloc with the main lesion
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grounded within selfdetermination theory sdt deci ryan ryan deci in press three studies were conducted to develop and psychometrically test a measure of adolescents perceptions of psychological need support for exercise viz for autonomy competence and relatedness the adolescent psychological need support in exercise questionnaire apnseq in study items were developed in collaboration with an expert panel through categorical CFA and item response theory responses from adolescents were used to identify the best fitting and performing items in study here a threefactor nineitem measure showed good fit to the data in study responses from an independent sample of adolescents provided further evidence for the nineitem solution as well as for internal consistency criterion validity and invariance across gender and social agent friends family and PE teacher the apnseq was supported as a measure of adolescents perceptions of psychological need support within the context of exercise
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changes in lung hyperinflation dyspnea and exercise endurance are important outcomes in assessing therapeutic responses in chronic obstructive pulmonary disease copd therefore we studied the reproducibility of borg dyspnea ratings inspiratory capacity ic to monitor lung hyperinflation and endurance time during constantload symptomlimited cycle exercise in patients with copd fev predicted mean sem responsiveness was also studied by determining the acute effects of nebulized micrograms ipratropium bromide ib or saline PL p on these measurements during each of four visits conducted over an wk period spirometry and ET were performed before and h T3 receiving ib or p randomized doubleblinded highly reproducible measurements included Tlim ICC r p borg ratings and ic at rest at a standardized exercise time std and at peak exercise r p and slopes of borg ratings over time oxygen consumption v o and ventilation r p responsiveness was confirmed by finding a significant drug effect for change delta in Tlim p deltaborgstd and deltaborgtime slopes p and deltaic at rest at std and at peak exercise p with all completed visits deltaborgstd correlated better with deltaicstd than any other resting or exercise parameter n r p we concluded that borg dyspnea ratings and measurements of ic and endurance time during submaximal cycle ET are highly reproducible and responsive to change in severe copd
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elevated BPb bpbs have been identified in uruguayan children in the la teja neighborhood of montevideo but the extent of lead SE in other city Az is unknown sources and predictors of SE also remain understudied in this population in the authors screened lead and hemoglobin levels in capillary blood of preschool children from several Az of montevideo uruguay and identified predictors of elevated bpbs mean bpb was microgdl and of children had C2 or microgdl mean hemoglobin level was gdl with having levels gdl older child age hemoglobin gdl and putting fingerstoys in the mouth were associated with higher bpbs young maternal age less education fathers job with potential risk of lead exposure and fewer family possessions were also associated with higher bpbs pediatric lead SE is a public health problem in uruguay with children experiencing elevated bpbs at a young age
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human dilated cardiomyopathy dcm is characterized by congestive HR failure and altered myocardial gene expression epigenetic changes including dna methylation are implicated in the development of dcm but have not been studied extensively clinical human dcm and nonfailing control left ventricle samples were individually analyzed for dna methylation and expressional changes expression microarrays were used to identify overexpressed and underexpressed genes in dcm geo accession number gse gene promoter microarrays were utilized for dna methylation analysis and the resulting data were analyzed by two different computational methods in the first method we utilized subtractive analysis of dna methylation peak data to identify gene promoters exhibiting dna methylation changes that correlated with expression changes in the second method a twostage RPA combined a particle swarm optimization feature selection algorithm and a discriminant analysis via mixed integer programming classifier to identify differentially methylated gene promoters this analysis identified hypermethylated promoters and six hypomethylated promoters in dcm with CV accuracy in the group assignment generation of a composite list of genes identified by subtractive analysis and twostage computation analysis revealed four genes that exhibited differential dna methylation by both methods in addition to altered mRNA computationally identified genes aurkb btnl cldn and tk define a central set of differentially methylated gene promoters that are important in classifying dcm these genes have no previously reported role in dcm this T0 documents that rigorous computational analysis applied to microarray analysis of healthy and diseased hH1 samples helps to define clinically relevant dna methylation and expressional changes in dcm
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in a multicenter placebocontrolled doubleblind clinical study in elderly patients with cognitive decline nimodipine a calcium antagonist was found to be a therapeutically ERP agent in the treatment of old age dementias treatment with mg of nimodipine po orally in divided doses for weeks was significantly superior to an inactive placebo on all outcome measures including the wechsler memory scale the mini mental state examination the GDS the sandoz clinical assessment geriatric scale the plutchik geriatric rating scale the severity of illness and global improvement scales of clinical global impression and the hamilton psychiatric rating scale for depression adverse effects with nimodipine were few and mild the drug was equally well tolerated and equally ERP in the two major dementias of old age ie primary degenerative and multiinfarct the number of abnormal laboratory test readings remained essentially unchanged from pretreatment to PT
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severly increased AV is an important risk factor for patellofemoral instability recurrent dislocations cause a traumatic disruption of the medial patellofemoral ligament therefore a procedure that combines femoral derotation osteotomy and patellofemoral ligament reconstruction should be considered for patients with severely increased femoral anteversion the aim of the study was to evaluate the subjective and objective outcomes T3 combined femoral derotation osteotomy and anatomical reconstruction of the mpfl
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the PP bonding questionnaire pbq was developed to assess motherinfant bonding disturbances in the PP period the aim of this T0 was to examine the psychometric properties of the spanish version of the pbq in a sample of spanish PP women eight hundred forty mothers were recruited in the PP visit weeks after delivery from a gynecology unit forming the general population sample and mothers from a perinatal psychiatry program forming the clinical sample all women were assessed by means of the edinburgh PN depression scale epds and the pbq neither the original fourfactor structure nor alternative structures reck et al wittkowski et al were replicated by the confirmatory factor analyses an EFA showed a fourfactor solution the schmidleiman transformation found a general factor thatβ plays a vital role in the terminal processes of human labour and delivery inflammasome activation is required to process pro ilβ to an active secreted molecule recent studies have shown that autophagy regulates ilβ via the inflammasome the aims were to determine the effect of i human spontaneous term and PT labour on the expression of autophagy proteins in fetal membranes and ii autophagy inhibition on ilβ PR
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although the semantic SM impairment has been largely documented in alzheimers disease little is known about semantic memory in the preclinical phase of the disease mild cognitive impairment the purpose of this study was to document the nature of semantic breakdown using a battery of tests assessing different aspects of conceptual knowledge knowledge about common objects famous people and famous public events results indicate that all domains of semantic memory were impaired in mci individuals but knowledge about famous people and famous events was affected to a greater extent than knowledge about objects this DP of results suggests that conceptual entities with distinctive and unique properties may be more prone to semantic breakdown in mci in summary results of this study support the view that genuine semantic deficits are present in mci it could be useful to investigate the etiological outcome of patients failing or succeeding at such tests
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fasassociated factor faf is a multifunctional proapoptotic protein that is involved in fasmediated apoptosis nfkappab signalling and the ubiquitinproteasome pathway in the ubiquitinproteasome pathway faf binds to the n domain of pvcp a molecular chaperone that acts in complex with the proteasome through its cterminal ubx domain and inhibits the proteasomal proteindegradation process in an effort to elucidate the structural basis of the function of faf in modulating pvcp activity related to proteasomal PD crystallographic analysis of the faf ubx domain and the pvcp n domain was initiated following the recently reported crystallization of the faf ubx domain bound to the pvcp n domain the unbound faf ubx domain was also crystallized for purposes of structural comparison xray data were collected to a resolution and the crystals belonged to space group f with unitcell parameters a b c a the matthews coefficient and solvent content were estimated to be a da and respectively assuming that the asymmetric unit contained two molecules of the ubx domain which was subsequently confirmed by molecularreplacement calculations
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primary and M1 tumors of both soft tissues and bony skeleton and primary PT of adjacent organs invading the chest wall constitute CW tumors a retrospective review of all the patients with chest wall PT was done at bp koirala memorial cancer hospital bpkmch primary tumors of breast were excluded surgical treatment consisted of wide LE wle chest wall reconstruction if needed was achieved by a muscular flap prolene mesh omental transposition thirty one patients were treated in the period from october to october age of the patients varied from years to years mean age years presenting complaint was mass in and pain in cases the mass was cm or less in from to cm in and more than cm in cases the lesions were located in sternal region anterior lateral posterior and vertebral chest wall in and respectively wle was done in cases CW reconstruction using both muscular flaps and prolene mesh x cm was done in cases in three of them where concomitant wedge resection of the lung was done omental transposition was added in rest of the cases primary closure muscularmyocutaneous flap or skin grafting was done minor complications were observed in cases which were managed conservatively two patients received adjuvant radiotherapy and four patientsadjuvant chemotherapy there were no postoperative deaths the rate of malignancy was
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the records of neonatal deaths in a four year period were reviewed retrospectively sixteen patients developed multisystem organ failure msof the criteria for pulmonary hepatic renal hematologic cardiac and microvascular failures were established the onset of the first organ involvement was calculated in days prior to death the earliest organ involved was kidney followed by microvascular hematologic CL lung and cardiac failure blood SC analyses reveale promoter elements located to the initiation codon and a polyadenylation signal in the utr a distinctive codon usage bias for a or t in the third position and an at bias greater than in the flanking regions of the transcripts at the protein level both enzymes belong to the short variant NMP nmp kinases which lack a amino acid lid region present in the long variant isoenzymes ehak was identical to the members of the adenylate kinase ak family while ehuk was more similar identity to umpcmp kinases both enzymes used atp as preferred phosphategroup donor but each one exhibited strict specificity for the acceptor nmp ehak for amp and ehuk for the pyrimidine nucleoside monophosphates ump and cmp biochemical characterization of the enzymes and phylogenetic reconstruction showed that ehuk is an authentic and well conserved member of the umpcmp kinase group while ehak is the most divergent member known of the ak isoenzymes
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the cutaneous innervation of the thoracic limb was investigated in barbiturateanesthetized dogs using electrophysiologic techniques the cutaneous area ca innervated by each cutaneous nerve was delineated in at least five dogs by stroking the hair in the area with a small watercolor brush while recording from the nerve mapping of adjacent ca revealed areas of considerable overlapping the part of the ca of a given nerve supplied by only that nerve is referred to as its autonomous zone of all nerves arising from the BP only the suprascapular subscapular lateral thoracic thoracodorsal and cranial and caudal pectoral nerves lacked cutaneous afferents the dorsal cutaneous branch of c had a ca but no grossly demonstrable dorsal cutaneous branches for c c or t were found the C2 SN had VP cutaneous branches but no lateral cutaneous branches thoracic SN tt had dorsal ventral and lateral cutaneous branches the cutaneous branches of the brachiocephalic axillary musculocutaneous radial median and ulnar nerves all had ca which were overlapped by adjacent ca thus their autonomous zones were much smaller than the cutaneous areas usually depicted for these nerves in anatomy and neurology textbooks
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in this observational T0 we compared EA membrane fatty acids in infants consuming formula supplemented with docosahexaenoic acid dha and arachidonic acid ara with those consuming other types of milks in infants who were participants in a cohort study of otherwise HC at risk for developing type diabetes erythrocytes were collected at approximately months of age and fatty acid content was measured as a percentage of total lipids parents reported the type of milk the infants consumed in the month of and prior to erythrocyte collection infant formula supplemented with ara and dha supplemented formula formula with no ara and dha supplements nonsupplemented formula BM or nonsupplemented formula plus breast milk membrane dha versus p and omega fatty acid versus p C2 were higher in infants consuming supplemented versus nonsupplemented formula omega fatty acids were lower in infants consuming supplemented versus nonsupplemented formula versus p ara did not differ between CG infants given supplemented formula had higher dha versus p and omega fatty acids versus p than infants drinking breast milk in infants whose mothers did not receive any dietary advice use of supplemented formula is associated with higher omega and lower omega fatty acid status
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levels of urinary GCs and their concentration ratios have been analyzed as potential markers for various pathological statuses largescale studies may possibly accelerate the investigations however a suitable method needs to be established analytical conditions for measurement of urinary GCs with lcms were examined ESI in the positive ion mode was applied for detection of cortisol precursorproduct ion cortisold internal standard is and cortisone to maximize ionization acetic acidammonium acetate buffer mm at ph was employed as eluent a a c column mmÃmm μm at °c was used for the min binary gradient separation starting with eluent a with methanol being eluent b linear correlations were observed between the concentrations and the peak areas in the concentration range of ngml with rho coefficients r of and for cortisol and cortisone respectively without is adjustment and with is adjustment for both cortisol and cortisone solidphase extraction spe using a ml centrifuge column was performed for the urine samples with the original and final volumes being μl the spe of urine specimens could be performed within min the effect of the sample matrix on the quantification of endogenous EDC present in the urine extract was limited coefficient of R2 cv of isadjusted matrix factor urine extracts of individuals however substantial peak reduction of cortisol was observed at low concentrations exogenous contaminants originating from the spe centrifuge column seemed to be a main cause for this phenomenon because the purewater extract showed similar peak reduction a REC of â¼ was obtained for both cortisol and cortisone adjustment with the is improved the apparent REC with â¼ being obtained for both cortisol and cortisone the REC rate decreased when the urine samples were concentrated in the spe step the reduction was greater for cortisol than for cortisone the lower limit of quantification lloq was set at ngml when the injection volume was μl based on the reproducibility of the standards which were measured cv of repetitions for ngml cortisol and for ngml cortisone the matrix effect at ngml concentrations of cortisol and the REC rate â¼ furthermore an alternative RPA for preparation of the cortisol standards was required for low concentration range ngml because of the effect of the matrix degradation of original urine specimens at RT was minimal during the first h the extracted urine samples degraded over time however their concentrations were corrected with the is allowing for analysis up to days after extraction in conclusion an analytical method for urinary glucocorticoids was established which is fast sensitive and well suited for practical application to largescale study
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in a previous study meiotic activity was observed in human intratesticular xenografts from peripubertal patients however full spermatogenesis could not be established the present study aimed to evaluate whether the administration of recombinant human fsh could improve the spermatogonial survival and the establishment of full spermatogenesis in intratesticular human xenografts human testicular tissue was obtained from six boys aged years the testicular biopsy was fragmented and one fragment of mm was Tx to the testis of nude nude mice transplanted mice were assigned to different experimental CG to enable evaluation of the effects of fsh administration and freezing the structural integrity of the seminiferous tubules the spermatogonial survival and the presence of differentiated cells were evaluated by histology and immunohistochemistry freezing or administration of fsh did not influence tubule integrity and germ cell survival in human xenografts meiotic germ cells were observed in the xenografts more tubules containing only sertoli cells were observed in frroved the benefits of the proposed method by comparing the classification results with and without F0 over regional myocardial segments obtained from subjects the evaluations in comparisons to the ground truth classifications by radiologists showed that the proposed F0 yielded an areaunderthecurve auc of bringing a significant improvement of in comparisons to previous methods that use only shortaxis images
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background paradoxical reactions to tuberculosis tb are clinical or radiological worsening of prior tuberculous lesions or the development of new lesions upon treatment with AGA antituberculosis therapy att this phenomenon has been described in both hivseropositive and HIV- patients although historically estimated to occur in of hivseronegative patients with tb the phenomenon is often underrecognized in the current era particularly in countries of low tb prevalence we describe a case of a tb paradoxical reaction affecting the cns and spine in an hivseronegative individual who received clinical care in the us case report a yearold hivseronegative refugee from eritrea presented to the hospital with fever back pain and headache shortly T3 arriving to the us he was diagnosed with tb meningitis and potts disease and was started on att he developed worsening clinical PS including headaches transient diplopia and mood disturbances as well as new radiologic abnormalities in the brain tuberculomas and spine abnormal enhancement despite AGA att he received prolonged drug att and CS as well as changes in his att regimen and multiple attempts were made to biopsy the brain and spine to address concerns for radiologic changes eventually he was discharged year later with clinical improvement and full neurologic recovery conclusions radiologic and clinical findings due to paradoxical reactions may be unfamiliar to clinicians in countries with low tb prevalence and inadvertently lead to either inadequate management such as the underappreciation of the clinical signs and PS indicating potential severity of cns paradoxical reaction or conversely overly invasive approaches in a patient who is otherwise clinically improving increasing awareness about extrapulmonary paradoxical reactions in such patients is crucial for ensuring appropriate diagnostic approaches and timely clinical management
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airpuff stimulation applied to a fingertip is known to exert a locationspecific facilitatory effect on the size of the motor evoked potentials elicited in hand muscles by TMS stimulation in order to clarify its nature and the pathway responsible for its generation we studied patients with discrete lesions in the brain and patients with lesions in the cerebral SC thalamus and brainstem respectively facilitation was absent in patients with lesions affecting the primary sensorimotor area whereas it was preserved in patients with cortical lesions that spared this area facilitation was abolished with thalamic lesions that totally destroyed the VPL vpl but was preserved with lesions that at least partly spared it lesions of the STT did not impair facilitation the size of the np component of the somatosensory evoked potential showed a mild rho with the amount of facilitation the facilitation is mainly mediated by sensory inputs that ascend the dorsal column and reach the SC through vpl these are fed into the primary motor area via the primary sensory area especially its anterior portion corresponding to brodmann areas and possibly also area without involving other cortical regions the spinothalamic tract and direct thalamic inputs into the motor SC do not contribute much to this effect some patients could generate voluntary movements despite the absence of the facilitatory effect the present method will enable us to investigate in humans the CF of one of the somatotopically organized sensory FB input pathways into the motor SC and will be useful in monitoring ongoing finger movements during object manipulation
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we report a case of presumptive neuroleptic malignant syndrome requiring muscle EC50 for ECT short acting muscle relaxation without the use of succinylcholine was achieved using rocvronivm reversed with the novel reversal agent sugammadex we suggest that this combination is a safe and ERP alternative to succinylcholine in such cases
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we present the case of a yearold patient without any past medical history admitted to our icu for cardiogenic shock complicating acute coronary syndrome the blood tests found polycethemia a polycethemia vera was suspected and confirmed by genetic analysis ischemic HF as an initial symptom of polycethemia vera and its treatment by arterial bleeding is a rare event that we describe in this article
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the present study evaluated the utility of the chinese version of the revised controlling behaviors scale ccbsr as a measure of controlling behaviors in violent chinese intimate relationships using a mixedmethods RPA indepth individual interviews were conducted with chinese women survivors to elicit qualitative data about their personal experiences of control in intimate relationships the use of controlling behaviors was also assessed using the ccbsr interview accounts suggested that the experiences of of the women were consistent with the description of coercive control according to dutton and goodmans conceptualization of coercion using the splithalf validation procedure a receiver operating characteristics roc curve analysis was conducted with the first half of the sample the area under the curve auc for using the ccbsr to identify high control was and the cutoff score of maximized both sensitivity and specificity applying the cutoff score to the second half gave a sensitivity of and a specificity of overall the ccbsr has demonstrated utility as a measure of controlling behaviors with a cutoff score for distinguishing high from low C2 of control in violent chinese intimate relationships
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genes encoding homologs of the herpes simplex virus type ul product glycoprotein m are conserved in all herpesviruses investigated so far recently we identified PR virus prv gm as a kda structural component of purified virions a gmprv mutant could be propagated in CC albeit at lower titers and with delayed penetration kinetics thus gm has a nonessential but modulatory function in prv infection prv gm is modified by addition of an nlinked glycan at a consensus CS located between the predicted first and second hydrophobic region of the protein this nglycosylation site is conserved in all gm homologs sequenced so far indicating an important PET role to analyze intracellular processing of prv gm western blot analyses were performed in prvinfected cells mature kda gm as well as and kda precursor forms were detectable presumably dimeric and kda proteins were also observed the and kda proteins represent nonglycosylated and glycosylated precursors as shown by endoglycosidase digestions investigation of several prv strains revealed that the ul product of prv strain bartha an attenuated virus used as vaccine was not modified by nglycosylation CS analysis showed that the nglycosylation consensus sequence was altered from ndt to nda which resulted in loss of the nglycosylation signal to our knowledge this is the only gm homolog identified so far which is not nglycosylated to investigate whether this form of the protein is functionally competent the ul gene of strain bartha was inserted into prv strain kaplan by substitution of the wildtype ul gene the resulting recombinant expressed a ul protein lacking nglycans in vitro replication analyses did not reveal any difference in virus production but PI size and penetration kinetics were slightly reduced in summary we show that wildtype gm is modified by nglycosylation at one conserved site however although this site is highly conserved throughout the herpesviruses loss of nglycans due to mutation of the consensus CS had only a minor effect on propagation of prv in CC
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in this article is presented a case of multiple chromosomal aberrations in a patient with cml accelerated phase cytogenetic and molecular cytogenetic studies allowed us to determine the presence of tqq and to identify additional abnormalities such as tpp del q delq delq der some of which are not typical for this kind of neoplasia this case is compared with publications of the same cases our data suggested that detected changes can be correlated with previous treatment regimens and the influence of these changes on progression of disease is discussed
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this report describes a cluster of soldiers with vivax malaria among us military personnel who trained at dagmar north training area near the demilitarized zone dmz in the ROK rok in two cases were diagnosed in the rok in one of whom subsequently experienced a relapse and nine other cases were diagnosed in months T3 the soldiers had returned to the us vivax malaria poses a health threat to us forces korea operating near the dmz in the rok continuing and enhanced focus on force health protection measures in endemic zones is warranted
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avian nephritis virus anv infection was detected in dayold to dayold chickens collected on hungarian farms between and the animals suffered from diarrhoea growth retardation runtingstunting syndrome and to mortality was reported tubulonephrosis interstitial nephritis and uricosis gout was diagnosed the presence of anv rna was detected in chicken carcasses using RT-PCR the virus was demonstrated in of the investigated farms the nucleotide sequence of the amplification products corresponding to part of the genome that encodes the gp protein was determined and phylogenetic analysis was performed the nucleotide sequences showed to identity to the reference CS isolated in in japan the constructed phlyogenetic tree indicates high diversity of the hungarian anv sequences regardless of their origin and year of sample collection analysis of the putative amino acid sequences encoded by the partial gp sequences also revealed high diversity of the virus even samples collected at the same farm at the same time but from different flocks differed in nucleotide and putative amino acid sequences the possible effects of the sequence diversity on the pathogenicity antigenicity and diagnostics of anv are discussed
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individuals experiencing nonsuicidal selfinjurious nssi thoughts only are greatly overlooked by current research this investigation aimed at determining how three groups of university students differed in their reported quality of childhood relationships with parents and histories of physical and sexual abuses these CG included students experiencing only nssi thoughts n students engaging in nssi actions n and students exhibiting neither n results showed that individuals experiencing nssi thoughts only and those engaging in nssi actions reported poorer relationships with parents and more physical abuse than the no nssi group however nssi thoughts and nssi action groups had similar outcomes to one another for most variables these findings suggest that individuals experiencing only nssi thoughts share similar negative childhood environments associated with engagement in nssi action and that they should be included in future research particularly investigations aimed at identifying protective AF that could prevent them from engaging in nssi
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the purpose of this work was to examine the ability of an oncolytic vaccinia virus expressing the human sodium iodine transporter hnis to provide real time monitoring of viral therapy and effective treatment of malignant pleural mesothelioma mpm
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a calciumactivated neutral proteinase canpspecific endogenous inhibitor calpastatin was purified from bovine brain by successive column chromatography the purified inhibitor exhibited a L1 band on sodium dodecylsulfate polyacrylamide gel PACE with an approximate molecular weight of kd the polyclonal antisera raised to the inhibitor strongly reacted with the kd protein band two lightly stained bands approximately kd and kd were also recognized by the inhibitor antiserum the inhibitor specifically inhibited canp activity and the halfmaximal inhibition was found with ng of calpastatin per micrograms of canp in a final volume of microliters cat b and papain were not inhibited by the inhibitor while trypsin and chymotrypsin were inhibited to some extent the inhibitor formed a complex with canp and the inactive complex was dissociated into active fractions of enzyme and calpastatin in the presence of egta
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we tested the hypothesis that the negative PET effects of cyclic gmp were mediated by ryanodine receptors and that these effects would be reduced in thyroxine thyroxine mgkgday daysinduced hypertrophic myocytes using rabbit VVI myocytes from control n and thyroxine n NF percent cell shortening and maximum rate of contraction and EC50 were determined using a video edge detector at baseline and after m bromocyclic gmp dantrolene m ryanodine receptor inhibitor was added CT or T3 brcgmp treatment changes in cytosolic ca concentration were assessed in furaloaded control and thyroxine myocytes brcgmp caused a significant decrease in percent shortening from to at m in control and to in thyroxine myocytes dantrolene significantly decreased percent shortening from to in control and from to in thyroxine myocytes in brcgmp treated control myocytes dantrolene did not significantly NC myocyte +dP/dt which suggested that cyclic gmp acted on RyRs however in brcgmp treated thyroxine myocytes dantrolene further reduced myocyte +dP/dt implying that the interaction of cyclic gmp and ryanodine receptors appeared to be interrupted in thyroxine myocytes maximum rate of contraction data were consistent with the percent cell shortening data and ca transients changed similarly to myocyte +dP/dt we conclude that effects of cyclic gmp on myocytes +dP/dt were partially mediated though interaction with ryanodine receptors and the subsequent decrease in cytosolic calcium levels this interaction was reduced in thyroxine hypertrophic myocytes
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nineteen pregnant women with a clinical suspicion of IUGR and with gestational age confirmed by early US were referred to our departments for sonographic evaluations multiple biometric parameters were obtained including the TCD by use of the electronic calipers of the machine a prenatal diagnosis of IUGR was made in all cases based on the TCD being consistently correlated with gestational age as predicted by the last menstrual period whereas most of the other measurements were consistently discrepant with the transverse cerebellar diameter by more than weeks ie more than sd above the mean and the estimated fetal weight of all fetuses being equal to or less than the tenth percentile for gestational age neonatal examination confirmed all fetuses to be growth retarded with birth weights at or below the tenth percentile for gestational age these findings indicate that growth of the transverse cerebellar diameter is unaffected by IUGR thus this sonographic measurement may serve as an independent and reliable correlate of gestational age against which potential deviations of growth may be compared
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spinal anterior horn and ventral roots from c of spinal cord patients with motor neuron disease mnd and those from controls were studied morphologically and biochemically using microscopic observation morphometry and microdensitophotometry spinal anterior horns in mnd showed various kinds of morphological changes in nerve cells and a significant decrease in cellular nuclear and nucleolar crosssectional areas in normal and abnormal cells microdensitophotometry revealed a significant decrease in the cellular rna content of both large cells and small cells and also of histologically normal appearing cells and abnormal cells those findings point to an abnormality of rna synthesis which precedes the earliest LM changes seen in nerve cells the crosssectional areas of myelinated fibers and axons of spinal ventral roots in mnd cases showed a significant decrease in the numbers of both total myelinated SF and large axons the abnormalities in myelinated SF and axons of spinal ventral roots in mnd cases might be AA to those in both large nerve cells and small ones of spinal anterior horns in most mnd cases andor primary in some cases
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measurements of nitrogen oxide and copper in the blood of patients with CVH c was carried out before antiviral therapy by electron paramagnetic resonance on a radiopan epr spectrometer the results indicate elevated C2 of nitrogen oxide and copper in the blood of these patients in comparison with normal subjects comparison of these findings with the results of a previous analysis of redox status of patients with cnal transmission capsaicin neurotoxin cgrp cgrp antagonist or bctc trpv receptor antagonist we measured GE and intestinal transit of evans blue next to rectal temperature and a global sickness behaviour scale in vehicletreated mice lps significantly delayed GE SIT and rectal temperature while the sickness behaviour scale was increased hexamethonium capsaicin cgrp and bctc all reversed the endotoxininduced delay in gastric emptying and significantly reduced the delay in intestinal transit without effect on the endotoxininduced decrease in rectal temperature and increase in sickness behaviour scale our findings provide evidence for the involvement of afferent nerves in the pathogenesis of endotoxininduced motility disturbances in mice mediated via cgrp and trpv receptors blockade of cgrp and trpv receptors may offer a novel strategy for the treatment of endotoxininduced ileus
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the aim of this study was to determine the relative frequency and CSD of reactive hyperplastic lesions rhl of the oral mucosa at the oral pathology institute of the school of dentistry at the university of chile this was a retrospective study of biopsies with histopathological diagnosis of rhl performed between and the rhl were classified in groups fibrous PH fh pyogenic granuloma pg IP giantcell granuloma pgcg and peripheral POF pof
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no standard method for handling and histopathologic examination of the sentinel node sn exists we hypothesized that a focused examination of all NO with serial sectioning and cytokeratin immunohistochemical IF would confirm the sn as the node most likely to harbor metastasis intraoperative LM and sentinel lymphadenectomy using blue dye and mtechnetiumlabeled sulfur colloid were performed all NO were stained with he all tumorfree nodes underwent additional sectioning and IF with he and an immunohistochemical stain routine he examination detected sn metastases in of ate this difference we conducted a T0 employing the radiographs of patients who visited nihon university dental hospital at matsudo and were pathologically defined as having a cyst or RT using radiographs of these patients we investigated the att point to the embedded tooth and expressed the results as the proportion of the attachment point to the embedded tooth root length the T0 was carried out in patients with cysts dentigerous cysts and odontogenic keratocysts and patients with benign PT ameloblastomas and three adenomatoid odontogenic PT prior to treatment based on the numerical results the CSD of the results was examined thus we evaluated several methods of examining the distributions and found the best method to be DA the results showed that the discriminated boundary value from the cementoenamel junction was for the embedded tooth root length the cases showing a boundary value of less than for the cementoenamel junction were judged to be cysts and those showing a value of or more were judged to be benign PT the rate of misjudgement was in the cyst group and in the benign tumor group
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the aim of the study was to investigate the biocompatibility and osteogenic effectiveness of the porous nanohydroxyapatitepolyamide nhapa scaffold material that was cultured with the rat bone marrow mesenchymal stem cells rbmscs under the static culture condition and the dynamic perfusion SC condition in vitro and to investigate whether the d perfusion SC condition was better in provoking proliferation of rbmscs than the d static culture condition the methyl thiazolyl tetrazolium mtt assay ALP phosphatase alp activity CA osteocalcin ocn assay and SEM TSM sem were used to observe the proliferation and differentiation of rbmscs the samples were respectively harvested at st rd th th and st days and effect comparisons were made between the two of the SC conditions the results showed that values of mtt alp and ocn were increased continuously and revealed a significant difference between the two SC conditions p on the th day sem revealed calcified nodules μm in diameter favorable for stability in a number of cases since control of ph is widely provided by added buffer salts the current study summarizes the buffer characteristics of acetate citrate histidine succinate and phosphate buffers experimentally derived values largely coincide with values calculated from a MM that had been proposed in by van slyke as high concentrated protein formulations become more and more prevalent for biologics the selfbuffering potential of proteins becomes of relevance the current T0 provides information on buffer characteristics for ph ranges down to and up to and shows that a monoclonal antibody at mgml exhibits similar beta as mm citrate or mm histidine ph buffer capacity of antibody solutions scales linearly with protein concentration up to more than mgml at a protein concentration of mgml the buffer capacity resembles the buffer capacity of mm citrate or mm histidine ph the buffer capacity of monoclonal antibodies is practically MZ at the process relevant temperatures and °c changes in ionic strength of δi in contrast can alter the buffer capacity up to in conclusion due to efficient selfbuffering by antibodies in the ph range of favored chemical stability conventional buffer excipients could be dispensable for ph stabilization of high concentrated protein solutions
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ICE activation plays a CE role in the execution of apoptosis the key components of the biochemical pathways of ICE activation have been recently elucidated in this review we focus on the two most wellstudied pathways of ICE activation the cell surface DR pathway and the mitochondriainitiated pathway in the cell surface DR pathway activation of ICE following its recruitment to the deathinducing signaling complex disc is the critical event that transmits the death signal this event is regulated at several different levels by various viral and rat proteins G1 caspase can activate downstream caspases by direct cleavage or indirectly by cleaving bid and inducing cytochrome c release from the mitochondria in the mitochondrialinitiated pathway ICE activation is triggered by the formation of a multimeric apafcytochrome c complex that is fully functional in recruiting and activating procaspase activated ICE will then cleave and activate downstream caspases such as ICE and this pathway is regulated at several steps including the PR of cytochrome c from the mitochondria the binding and hydrolysis of datpatp by apaf and the inhibition of ICE activation by the proteins that belong to the inhibitors of apoptosis iap
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onehundredtwo urine and nasal samples collected from leprosy patients of different classifications of disease were studied for the presence of mycobacterium leprae antigens including phenolic glycolipidi pgli lipids were extracted from the urine samples and nasal washings were concentrated and used as such in the dotelisa two types of primary antibodies a polyclonal antibody obtained from lepromatous ll leprosy patients pooled and absorbed sera and an antipgli monoclonal antibody were used for the detection of m leprae antigens from these samples the polyclonal sera detected to of the PB pb and of the MB mb leprosy cases from the urine samples corresponding values for nasal PCD were to for pb and for mb cases the monoclonal antibody against pgli could not detect tuberculoid tt leprosy cases from the urine samples however of the BT bt of the BL bb of the borderline lepromatous bl and of the ll leprosy cases were detectable it was interesting to note that pb skinsmear negative cases were detectable from urine examination the specificity and sensitivity of the test is discussed in relation to the crossreacting antigens
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rat cardiac ventricular myosins were obtained from fetuses from young normal animals and from hypophysectomized adults the purified proteins were compared by several techniques i electrophoresis in nondenaturing conditions pyrophosphate buffer ii one and twodimensional analysis after proteolytic cleavage iii immunological blotting after electrophoretic purification and iv competitive enzymelinked immunosorbent assay antibodies TPS to each of the two L1 isoenzymes of adult rat HR v and v according to the terminology of hoh et al hoh j f y mcgrath p a and hale p t j mol cell cardiol were used for the immunological studies the heavy chains of the ventricular S1 isoenzymes of fetuses vf were indistinguishable from those of the vh isoenzyme present in hypophysectomized adults both proteins differed from the v isoform of young animals the CS chains of vf vh and v were the same except that vf contained in addition a small amount of the embryonic light chain whalen r g and sell s m nature these results strongly suggest that adaptation of the AD rat HR to the hormonal deficiencies of hypophysectomy is mediated by the synthesis of the same S1 heavy chain form which is predominant in fetal NF
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sky polarization patterns can be used both as indicators of atmospheric turbidity and as a sun compass for navigation the objective of this study is to improve the precision of sky CS polarization measurements by optimal design of the device used the CE part of the system is composed of a charge coupled device ccd camera a fisheye lens and a linear polarizer algorithms for estimating parameters of the polarized light based on three images are derived and the optimal alignments of the polarizer are analyzed the leastsquareturally INF cows in the asymptomatic subclinical n and symptomatic clinical n stages of disease as well as noninfected control cows all milk samples were assayed for map by culture on herrolds egg yolk medium and either bactec b becton dickinson franklin lakes nj or parajem thermo fisher scientific trek diagnostic systems inc cleveland oh liquid medium and by direct pcr for the is target gene mycobacterium avium ssp paratuberculosis was detected in and of milk samples collected from cows with subclinical jd T3 culture in herrolds egg yolk medium liquid medium and direct pcr respectively the frequency of map positivity increased to and of milk samples collected from cows with clinical jd by these same methods respectively none of the milk samples collected from control cows was positive for map by any detection method viable map was primarily isolated from milk and colostrum of subclinically and clinically infected cows collected in early lactation dim with negligible positive samples observed in mid dim and late dim lactation this T0 demonstrates that shedding of map into milk is affected by infection status of the cow as well as stage of lactation providing useful information to producers to help break the cycle of infection within a herd
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an elevation of phosphoglycerate mutase pmg has been detected in the blood plasma of the genetically dystrophic chicken and in duchenne muscular dystrophy dmd patients in the dystrophic chicken plasma pgm in the pectoral muscle was simultaneously depressed to less than onehalf that of the normal chicken in a group of dmd patients plasma pgm activity was found to be significantly raised above the normal range a survey of a small group of plasma specimens from human fetuses at risk for muscular dystrophy also suggested that pgm merits investigation as a potential adjunct to other diagnostic indices
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the effects of the calcium antagonist nisoldipine on contractions stimulated by phenylephrine and bht agonists of alpha and alpha ARs in isolated aortic rings from strokeprone spontaneously hypertensive rats shrsp and from normotensive WK wky were investigated in vitro phenylephrine and bht produced concentrationdependent contractions of vessels from both groups of animals the absolute force of the contractions was less in the aortae from hypertensive rats after all doses of both agonists nisoldipine inhibited the bht induced contraction much more in vessels from shrsp than in those from normotensive wky rats ic x versus x gml the phenylephrine contractions were inhibited in shrsp aortae by higher concentrations ic x gml of nisoldipine in wky nisoldipine only produced a slight inhibition of phenylephrineinduced contractions the inhibitory concentrations of nisoldipine on bhtinduced contractions are similar to those for the inhibition of the calcium or depolarizationinduced contractions in other experiments the alpha agonistinduced contractions of RA are dependent on TM calcium supply the higher efficacy of nisoldipine in aortae from shrsp suggests an increased transmembrane availability of calcium ions in hypertension
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the f hybrid transplantation law states that f hybrids of two unrelated inbred strains of mice accept grafts from either parent strain while neither parent accept grafts from the B/W however there are two notable exceptions to this law indeed parent grafts containing immunologically competent cells SP cells bone marrow etc react against the f normal hosts in which they are placed in the absence of a hostversusgraft reaction the graftversushost reaction gvh may dominate the field and cause untoward reactions which not only negate any benefit derived from the graft but also cause significant and often fatal morbidity of the host that is a gvh disease gvhd furthermore irradiated f mice are refractory to the grafting of parental bone marrow lymphoma cells and normal lymphocytes because of a restriction by noncodominant besides that of codominant mhch genes these noncodominant genes have been designated hh genes for hybrid H-2 and the phenomenon itself HR the cellular humoral and genetic mechanisms involved in the gvh and hybrid resistance production are explained and discussed in the first while in the second section of the present review their equivalent in three human pathological situations are taken into consideration we focus in detail on two principal immunologic aspects of human bone marrow transplantation gvh and hematopoietic engraftment each one of these immunologic aspects has considerable impact on the course and outcome of marrow transplantation in humans the allograft implantation between hlageneticallyidentical siblings hlaaploidentical family members or hlaphenotypically identical donorrecipient couples are herewith examined while the ABMT rescue RPA is not taken into consideration the outcome of bone marrow transplantation depends not only on the degree of genetic disparity between the donor and the recipient but also on the underlying disease bone marrow transplantation is the therapy of choice for patients with AA severe combined immunodeficiency scid and other genetic and acquired immunodeficiency some leukemias and a few other diseases mainly if hlageneticallyidentical siblings are available however in many cases such siblings are not available therefore the probability of acute or chronic gvhd or VG rejection increases significantly the clinical and pathologic manifestations the prognostic factors and the treatment and prevention of acute and chronic gvhd are illustrated in detailabstract truncated at words
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saccaadic response preferences and latencies were measured using dual targets presented at and deg along the horizontal meridian in either one or both visual hemifields in the unihemifield condition subjects exhibited a strong preference about in favor of the target presented closer to the original fixation point further the presence of the T2 did not increase the response latency to the preferred target in the bihemifield condition using spatially symmetric targets directional preference varied across subjects from to mean there was also a significant increase in response RL as compared with responses to either single targets or dual targets presented in one hemifield this latency increase was observed for responses in both the preferred and nonpreferred directions and was taken as evidence of mutual interaction between the two hemispheres the response preferences could be eliminated by delaying the onset of the preferred target by about msec in the unihemifield condition and by about msec in the bihemifield condition further the interactions between the effects of the targets on response time in the bihemifield condition were found to be asymmetrical in that a target presented in the preferred hemifield has a greater effect than a target in the nonpreferred hemifield on responses to targets presented in the counterhemifield the results suggest an asymmetry in hemispheric interaction which has not previously been demonstrated in the visualoculomotor system
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this contribution reports particleincell numerical studies of deuteron beam acceleration by a ps laser pulse of circular polarization the effect of laser wavelength λ and the ilλ product il is laser intensity on the ion beam parameters is investigated it is shown that at the ilλ product fixed the beam parameters ei ii fi as well as the laserions SE conversion efficiency quickly increase h lysosomes and proteasomes were upregulated selectively in axons by ngf stimulation of the distal axons of sympathetic SN suggesting that axonal growth and maintenance requires local protein turnover the regulation of the abundance of both proteasomes and lysosomes in axons by ngf provides a link between protein Kd and the trophic status of peripheral SN inhibition of proteasomes located in axons resulted in an accumulation of ubiquitinated proteins in these axons in contrast lysosome inhibition in axons did not result in an accumulation of ubiquitinated proteins or the transferrin receptor a TM protein degraded by lysosomes interestingly lysosomes were transported both retrogradely and anterogradely so it is likely that ubiquitinated proteins that are normally destined for degradation by lysosomes in axons can be transported to the cell HB for Kd in summary proteasomal Kd occurs locally whereas proteins degraded by lysosomes can most likely either be degraded locally in axons or be transported to cell HB for Kd
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in a recent T0 we described a group of monoclonal antibodies that identify five high molecular mass proteins which associate with intermediate filaments in the cytoplasm and accumulate in nuclear foci as well the proteins have been designated pndcfs proteins associated with nuclear dots and cytoplasmic filaments their expression in human B1 was upregulated by epsteinbarr virus ebv infection or by exposure to anticd antibodies and il phenotypically representative type i burkitts lymphoma bl cell lines do not express pndcfs or if they do the proteins accumulate preferentially in nuclear dots type iii bl cell lines that have drifted to a more immunoblastic phenotype during in vitro passage and ebvtransformed lymphoblastoid cell lines lcls of nonneoplastic origin express these proteins regularly at high C2 they are preferentially but not exclusively associated with vimentin filaments in the cytoplasm here we show that all five pndcfs can be upregulated by expressing the ebvencoded membrane protein lmp in type i bls three of them could also be upregulated in the human keratinocyte cell L1 rhek by lmp transfection this upregulation was paralleled by the lmpinduced increase in vimentin expression in both cell types one of the pndcfs detected by the mab dma accumulated in caplike structures under the CM that colocalized with membrane patches of lmp in addition to its localization in nuclear dots and in association with cytoplasmic vimentin filaments
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elevated cardiac troponin t ctnt has been associated with shorter survival in hemodialysis patients moreover intravenous iv iron treatment has been held responsible for oxidative AS and accelerated atherosclerosis in these patients in the present study we investigated the relationship between ctnt concentration iv iron treatment and parameters of iron status in addition parameters of oxidative AS inflammation and atherosclerosis were evaluated predialysis blood samples of chronic hemodialysis patients were analyzed for ctnt malondialdehyde creatine kinase ck and ckisoenzyme mb ckmb in addition the mean value of predialysis SS samples collected during the last year were considered for homocysteine ferritin iron iron IB capacity blood cell counts blood UN creatinine Al erythrocyte sedimentation rate esr creactive protein crp calcium phosphate ipth cholesterol and triglyceride the quantity of iv iron sucrose administered during the last two years was counted from the patients files echocardiography all events related to ICM HR disease and urine volume were also recorded elevated ctnt C2 or ngml were found in patients the amount of iron po was mg with a range mg patients with elevated ctnt levels received more iv iron than those with normal ctnt vs mg p the SS FER level was higher in patients with elevated ctnt median C2 vs ngml p patients with elevated ctnt were longer on dialysis compared to those with normal C2 median times vs months p and regression analysis identified the amount of po iron as an independent factor for elevated ctnt p intravenous iron treatment and high ferritin concentration are related to high ctnt level which has previously been incriminated as a survival marker in HD
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to assess the risk of pseudophakic cystoid macular edema pcme following cataract surgery in patients with allergies andor atopic disorders
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HGEC gec contain cd DAF and membrane cofactor protein crry is the rodent analogue to the latter two proteins we have previously shown that the nephritogenic ab of PHN antifxa impairs c regulation in rat gec here we examined rat gec c regulation ilabeled gec membrane proteins were immunoprecipitated with anticrry anticd or antifxa crry and cd were present in gec antifxa reacted with both crry and cd from gec as well as with purified rcrry and cd the alternative c pathway was studied by incubating gec in rat serum in mgegta buffer to inhibit the function of the c regulators anticrry or anticd ab were added to gec inhibition of cd CF alone had no effect on c regulation whereas inhibition of crry led to significant cytotoxicity from alternative pathway activation under conditions in which crry was inactive inhibition of cd further enhanced cytotoxicity when the CP pathway of c was activated by gecbound igg ab inhibition of either crry or cd enhanced cytotoxicity whereas inhibition of both crry and cd together was additive therefore crry and cd are present and functionally active in gec crry restricts c activation via both alternative and CP pathways when the CP pathway of c is G1 or when crry CF is inhibited cd limits cbmediatstaining decreased in dayold animals but recovered to the neonate levels in dayold animals homogeneity in the immunopositive population increased with age maintenance in culture for week resulted in an increase in positive somatostatin IF in animals of and days with no changes in neonates however after weeks of SC the percent of immunopositive cells decreased from to in the older animals and remained unchanged in neonates we conclude that somatostatin is found in pinealocytes and shows a declining DP during the perinatal period this probably implies that the peptide plays a paracrine role important for cell differentiation in these young animals since maximal cellularity and a high mitotic index occur within the first days of life and pineal cell differentiation is completed before the end of the third week of extrauterine life
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the purposes of this review are to assess the human exposure and human and experimental evidence for adverse effects of brominated flameretardants bfrs with TPS focus on NI from seafood the leakage of bfrs from consumer products leads to SE of humans from fetal life to adulthood fish and fish products contain the highest levels of bfrs and dominate the dietary NI of frequent fish eaters in europe while meat followed by seafood and dairy products accounted for the highest us dietary NI house dust is also reported as an important source of exposure for children as well as adults the C2 of bfrs in the general north american populations are higher than those in europe and japan and the highest C2 are detected in infants and toddlers the daily intake via BM exceeds the rfd in of us infants bfrs including pbdes hbcds and tbbpa have induced endocrine reproductive and behavior effects in laboratory animals furthermore recent human epidemiological data demonstrated association between exposure to bfrs and similar adverse effects as observed in animal studies fish including farmed fish and crude FO for human consumption may contain substantial C2 of bfrs and infants and toddlers consuming these products on a daily basis may exceed the tolerable daily intake suggesting that fish and fish oil CT represent a risk to human health this NI comes in addition to SE from other sources breast milk other food HD because potential harmful concentrations of bfrs and other toxicants occur in fish and fish products research on a wider range of products is warranted to assess health hazard related to the contamination of fish and fish products for human consumption
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xlinked inhibitor of apoptosis protein xiap has been shown to be highly expressed in lung CA but not in normal lung tissue which makes it an attractive target for lung CA treatment celecoxib cxb a cyclooxygenase inhibitor is in wide clinical use for the treatment and prevention of nonsmall cell lung CA nsclc therefore in our T0 we combined short hairpin rna shrna targeted to xiap xiapshrna with cxb and tested the effects of this combination on LC cells to identify more effective therapeutics against LC an xiapshrna plasmid was constructed and transfected into the a nsclc cell L1 the cells were then treated with cxb and xiapshrna alone or in combination for indicated time periods and the treatments were assessed for their effects on cell proliferation apoptosis migration invasion and receptor signaling using the mtt tunel wound healing and matrigel invasion assays and WB respectively in addition an nsclc xenograft model was prepared to observe RT growth it was found that both cxb and xiapâshrna significantly inhibited cell proliferation migration and invasion and induced cell apoptosis in vitro as well as suppressed RT growth in vivo moreoverg ever heard of tb was of those who ever heard of tb believed that tb can be cured and would want a family members tb to be kept secret of those who ever heard of tb believed that tb was spread from person to person through the air by coughing or sneezing multivariate analysis indicated that the probability of having poor knowledge of and negative attitude towards tb was consistently significant among the poorest household lowest wealth quintile geopolitical regions north central respondents with no schooling nonworking respondents youngest agegroup years and rural areas adjusted odds ratios aor ci for respondents who had ever heard of tb aor ci for respondents who had ever heard of tb and believed that tb can be cured aor ci for those who had ever heard of tb and concealed the fact that a family member had tb and aor ci for those who had ever heard of tb and believed tb was spread from person to person through the air by coughing or sneezing efforts to improve the knowledge of and attitude towards tb in nigeria should focus on the youngest agegroup years the poorest households and respondents with no schooling improving the knowledge and attitude of these groups of individuals may result in an increase in the number of people who will seek early treatment
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we determined the extent to which ligating both maternal uterine arteries affects fetal hepatic SE and redox states in the fetal rat bilateral maternal uterine i.a. ligation on d of the rats d gestation significantly inhibits fetal growth sham surgery limits growth to a lesser extent within h of surgery and persisting to d smallforgestational age sga fetuses had significantly diminished atpadp and adenylate charge ratios whereas sham fetuses had values intermediate between sga and normal hepatic mitochondrial redox state demonstrated similar changes cytosolic redox state in sga fetuses at and h after surgery was significantly elevated sga fetuses had significantly diminished plasma insulin and elevated glucagon concentrations on d and hepatic atpadp and cytosolic nadnadh correlated directly for sham and normal but not sga fetuses alterations in gl insulin and glucagon availability and hypoxia were responsible for the changes in SE and redox states they may also have disassociated hepatic cytosolic from mitochondrial redox states and altered the equilibrium between adenine and nicotinamide nucleotides these altered cellular functions retarded fetal growth newborn sga sham and normal rat pups had similar hepatic atpadp cytosolic and mitochondrial redox states at and min T3 delivery suggesting that the hypoglycemia which developed in sga pups was not attributable to alterations in these variables
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in a crosssectional evaluation of women belonging to the material of the leipzig longitudinal study of the gdr gerontology project foot trouble and objective foot changes were studied in relation to body weight and age the subjects were divided into four age classes and two weight CG the following objective foot parameters were obtained from the podogram the angle of the big toe the isthmus value and the lengthbreadth index further data such as the total foot angle the height of the plantar arch and the instep height were derived from the lateral radiograph of the foot under load in per cent of the subjects foot trouble was established two frequency peaks years and years were determined for the age at which the first symptoms manifested themselves the investigations showed a growing influence of physiological changes due to ageing on foot trouble the dependence of foot trouble on body weight is evidenced by the fact that overweight women are more prone to develop foot trouble than women with standard weight the angle of the big toe in particular is clearly weightrelated ie the risk to develop hallux valgus is greater in overweight women over years of age than it is in standardweight females this finding is corroborated by the fact that on an average the group of overweight subjects has lower lengthbreadthindex values which is indicative of a broadening of the forefoot in the process of splayfoot development
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hepatitis cassociated liver failure is the most common indication for CL transplantation histologic evidence of recurrence is apparent in approximately of HC virus hcvinfected recipients in the first postoperative year approximately of hcvinfected recipients will die or lose their allograft due to SH cassociated allograft failure hcvinfected recipients who undergo retransplantation have year patient and GS rates that are broadly similar to those for SPK recipients who are not hcv infected although the choice of calcineurin inhibitor mycophenolate mofetil or both has not been clearly shown to affect histologic recurrence of SH c higher cumulative exposure to CSs is associated with increased mortality and more severe histologic recurrence in contrast to treatment of nonhcvinfected recipients treatment of hcvinfected SPK recipients for acute cellular rejection is associated with attenuated patient survival SRR with or without the use of tcelldepleting therapies is associated with a greater than fivefold increased risk of mortality in hcvinfected CL Tx pegylated interferon with or without ribavirin should be considered for treatment of recipients with histologically apparent recurrence of SH c before total bilirubin exceeds mgdl the role of SH c immunoglobulin and new IS agents in the management of HC T3 transplant continues to evolve
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subjects suffering from late onset epilepsy were examined the incidence of CA defined with objective criteria on ct scans by means of a comparative analysis with a matched for age control group was head traumas cerebrovascular disorders and ALC were the presumed etiological AF in most of the cases while in subjects the etiology of CA remained unknown cryptogenous atrophy clinical profil psychometric performance and eeg characteristics of these subjects were investigated and compared with the remainder groups of lateonset epileptics the results obtained show that the subjects with cryptogenous atrophy behave as regard the features considered as the lateonset epileptics with normal ct but present an higher familial prevalence for epilepsy on the contrary they differs in various ways from lateonset epileptics with atrophy of known origin and from epileptics with focal lesions
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the aim of this T0 was to estimate the heritability of semen freezability and to estimate the genetic correlation between FT sperm traits and the growth rate in a paternal rb line estimated heritabilities showed that frozenthawed semen traits are heritable ranged between and in the case of liveft percentage of viable sperm T3 freezing the estimated heritability is the highest one and suggests the possibility of effective selection after the study of genetic correlations it seems that daily weight gain dg was negatively correlated with sperm freezability but no further conclusions could be drawn due to the high hpd more data should be included in order to obtain better accuracy for the estimates of these genetic correlations if the results oal maximum with an expected effectiveness of inferred from literature results based on empiric care provided within a hour period the mean number of infantinfant mrsa transmissible opportunities per hour was compared to no hand hygiene and averaged across all initial colonization states colonization was reduced by approximately and for the respective C2 of hygiene and preterm infants had a increase in mrsa colonization and MV infants had a increase conclusions even under optimal hygiene conditions horizontal transmission of mrsa is possible additional prevention paradigms should focus on the most acute patients because they are at greatest risk infect control hosp epidemiol
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anesthesia consists of three components unconsciousness analgesia and neuromuscular blockade nmb a specific drug is po by the anesthesiologist to control these different components in this paper we propose a new system for monitoring the NMB in anesthesized patients during surgery neuromuscular blockade drugs are used routinely by clinicians to induce muscle relaxation in patients however the use of these drugs has some risks so an adequate monitoring of the effects of these drugs is essential this paper describes the relaxofon a nmb monitoring device based on phonomyography the relaxofon is composed of a hardware subsystem that records muscle sounds using microphones and a special circuit to filter out the noise and amplify the signal and a software subsystem that analyses the acquired signal we tested the ability of the system to record phonomyographic signals from the AP and the corrugator supercilii muscles we then ral drug resistance in chronic SH are complex and the original pols gene overlap has to be taken into account treatmentinduced hbv mutations in pol could indeed generate s mutants with subsequent modified antigenicity or increased cancer induction R2 of hbv and hcv env proteins combining high exposure to selective pressures and crucial functional roles require investigation in the context of diagnostic vaccination and treatment tools in this editorial a synthesis is performed of hbv and hcv env properties at the entry step and as antigenic proteins and the subsequent clinical impact
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the recent evolution in therapeutic options for acute coronary syndromes acs mandates early risk stratification in order to select the appropriate treatment strategy for individual patients simple clinical data derived from the patients medical history and physical examination a standard twelvelead electrocardiogram ecg and determinations of PSA markers of myocardial damage can be obtained in the emergency room and serve as a guide for deciding AGA medical management and optimal use of available resources even the most important classification of the acs is based upon a simple and dichotomous description of the ecg where the presence of stsegment elevation mandates an immediate attempt to restore coronary perfusion either pharmacologically or mechanically whereas its absence suggests pharmacological stabilization before further DUE across the whole spectrum of acs clinical PH data such as older age previous coronary events and diabetes and clinical variables such as higher HR rate lower blood pressure and higher killip class are the most powerful prognostic determinants at multivariate analyses derived from C1 databases the ecg adds significant and independent prognostic information using the analysis of qualitative direction of stsegment shift associated twave inversion and presence of conduction disturbances and quantitative number of leads involved amount of st segment shifts duration of qrs characteristics biochemical markers of myocardial damage have also been identified as independent predictors of events in addition retrospective analyses of PCTs have suggested that PSA markers might serve as a guide to select pharmacological therapy however how to best combine electrocardiographic and PSA data for immediate risk stratification remains to be further elucidated
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the applied biosystems a nucleic acid extractor automates isolation of either dna or rna from tissue or cells in culture we have found that several modifications to the manufacturers recommended protocol greatly improve the SQ of rna that can be routinely isolated from cells in culture these modifications include lysis of ML cells directly on plates centrifuging samples T3 homogenization to remove precipitable rnase contaminants and purging the instruments reagent lines with diethyl pyrocarbonate these simple modifications enhance both rna quality and reproducibility of yield
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a combination of exp data using o labelling fragmentation data together with metastable ion studies in a reverse sector MS from a previous T0 and ab initio reaction coordinate studies at the ccsdtgdpblypgdp level of theory have provided the following data concerning the formation of pho in the gasphase from energized systems phochno n all deltag values were calculated at k phocho effects an ipso smiles rearrangement deltagr kj mol barrier to TS deltag kj mol equilibrating the two oxygen atoms the smiles intermediate reverts to phocho which then undergoes an sni reaction to form pho and ethylene oxide deltagr kj mol deltag kj mol the formation of pho from energized phocho is more complex some of the pho formed originates via a smiles intermediate deltagr kj mol deltag kj mol this species reconverts to phocho which then fragments to pho by two competing processes namely a an sni process yielding pho and trimethylene oxide deltagr kj mol deltag kj mol and b a dissociation process giving pho DDE and formaldehyde deltagr kj mol deltag kj mol the other fifteen percent of pho is formed prior to formation of the smiles intermediate occurring directly by the sni and dissociation processes outlined above the T0 of two fragmentation pathways is supported by the presence of a composite metastable ion peak energized phocho fragments exclusively by an sni process to form pho and tetrahydrofuran deltagr kj mol deltag kj mol the smiles ipso cyclization deltagr kj mol deltag kj mol is ND in this system
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about of the amino acid CS of Hc S1 hr from the southeast asian horseshoe crab carcinoscorpius rotundicauda has been determined there is a difference of about between hr and CR sequences of chelicerate hemocyanin subunits from the american horseshoe crab limulus polyphemus and a tarantula eurypelma californicum however the immunologically MZ subunits hr and ht from tachypleus tridentatus japanese horseshoe crab show sequence difference based on the CAA sequences of hr and ht the divergence between c rotundicauda and t tridentatus occurred about million years ago in the case of horseshoe crab hemocyanin subunits it seems that the orthologous homologues in many rat subunits between species are immunologically detectable
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brassinosteroids brs are steroidal plant hormones that are essential for growth and OD it has been proposed that brs are synthesized via two parallel pathways the early and late c oxidation pathways according to the c oxidation status the tomato lycopersicon esculentum dwarf gene encodes a cytochrome p that has been shown to catalyze the c oxidation of deoxocastasterone to castasterone we isolated an arabidopsis ortholog atbrox gene of the tomato dw gene the encoded polypeptide has characteristics of ps and is classified into the cyp family the atbrox and tomato dw were expressed in yeast and the ability of the transformed yeast cells to metabolize deoxobrs was tested metabolites were analyzed by gas chromatographymass spectrometry both enzymes catalyze multiple steps in br biosynthesis deoxoteasterone to teasterone dehydrodeoxoteasterone to dehydroteasterone deoxotyphasterol to typhasterol and deoxocastasterone to castasterone our results indicate that the atbrox gene and the tomato dwarf gene encode steroidoxidases and that these enzymes have a broad substrate specificity this suggests that the br biosynthetic pathway consists of a metabolic grid rather than two separate parallel pathways
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bright light therapy is a method of maintaining or restoring the NK CR by assisting daytime awakening using bright lights postoperative delirium is one of the potential complications encountered by patients receiving postoperative care in the intensive care unit icu but there have been no studies on the use of light for the prevention of postoperative delirium the objective of this study was to examine whether the circadian rhythms of patients T3 surgery for oesophageal CA can be adjusted and whether the postoperative delirium crisis rate can be reduced by bright light therapy the subjects were patients operated on for oesophageal cancer in osaka UH T3 IC was obtained they were divided into a T0 group and a control group by a random sampling method T3 removal of the endotracheal tube the study group was exposed to light thhibited internally consistent responses thus the parahippocampal and mec input to the hippocampus can be controlled by the animals internal direction sense presumably reflected in the firing of head direction cells and become completely decoupled from external sensory input yet maintain internal coherence with each other and in GA with the place cell system of the hippocampus
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cacao swollen shoot virus is a member of the family caulimoviridae genus badnavirus and is naturally transmitted to theobroma cacao l by several mealybug species cssv populations in west african countries are highly variable and genetically structured into several different groups based on the diversity in the first part of orf which encodes the movement protein to unravel the extent of isolate diversity and address the problems of low titer and mixed viral sequences in samples we used illumina miseq and hiseq technology we were able to reconstruct de novo new complete genomes from cacao samples collected in the cocoa MRI of ghana crig museum and from the field samples collected in côte divoire or ghana based on the threshold of nucleotide divergence in the reverse transcriptaseribonuclease h rtrnase h region which denotes species demarcation we conclude there exist seven new species associated with the cacao swollen shoot disease these new species along with the three already described leads to ten the total number of the complex of viral species associated with the disease a sample from sri lanka exhibiting similar leaf symptomology to west african cssdaffected plants was also included in the T0 and the corresponding sequence represents the genome of a new virus named cacao bacilliform srilanka virus cbslv
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ruptured aneurysms of the sinus of valsalva may cause manifestations of prompt onset a yearold man with no previous cardiac complaints presented with dyspnea palpitation fatigue and shortness of breath all of which occurred after a football match transthoracic echocardiography showed an aneurysmal dilatation of the RA sinus of valsalva to the direction of the RA IVT color doppler imaging showed a marked lefttoright turbulent flow from the aortic root to the right IVT continuous doppler imaging also revealed a continuous lefttoright systolodiastolic shunting the patient was submitted to cardiovascular surgery for surgical repair
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