text
stringlengths 10
10.1k
| source
stringclasses 1
value |
|---|---|
the three main dimensions of SQ of life physical function social activities and psychological status were evaluated by a questionnaire in copd patients with severe respiratory failure treated by LT oxygen the results were compared with those obtained in patients suffering from copd without chronic respira aims to provide just the RA amount of calories and in particular to limit the amount of saturated fatty acids ingested while providing suitable carbohydrates daily nutrition is taken in the form of several small meals ingested throughout the day such a diet can not only normalize blood gl but can also help reduce such diabeticrelated risks as overweight insulin resistance dyslipoproteinemia and hypertension
|
MEDAL
|
dhori and thogoto viruses have been isolated from ticks in portugal in this country favourable ecologic conditions exist for other tickborne viruses such as bhanja and congocrimean haemorrhagic fever cchf viruses a serological survey for antibodies to these tickborne viruses in human and goat sera was done using the plaque reduction neutralization prn and indirect FL antibody ifa tests six human sera were found with antibodies to dhori thogoto and cchf viruses while seven goat sera had NT antibodies na to dhori or bhanja virus from these results it seems likely that bhanja and cchf not yet isolated in portugal may exist focally in certain areas of southern portugal
|
MEDAL
|
the attd mouse pituitary tumor cell secretes corticotropin acth in response to corticotropinreleasing factor crf isoproterenol and vasoactive intestinal peptide vip these responses are associated with a rapid increase in cAMP formation somatostatin srif markedly decreases the stimulatory effect of crf isoproterenol and vip on both cyclic amp formation and immunoreactive acth secretion forskolin and cholera toxin adenylate cyclase activators also stimulate cAMP formation and acth secretion in att cells and these responses are all inhibited by srif the acth sec responses to melittin and to the calcium ionophore a neither of which increases cyclic amp in att cells were not inhibited by srif srif did not affect the binding of a tritiated betaadrenergic receptor antagonist to att CM nor did it decrease basal cAMP formation even in the presence of excess phosphodiesterase inhibitor indicating that the reduction of cyclic amp levels by srif did not involve either an interference with betaadrenergic agonist binding to receptors or stimulation of cyclic amp Kd these results indicate that the inhibition of crf isoproterenol and vipstimulated acth secretion by srif may be regulated by its inhibitory action on adenylate cyclase
|
MEDAL
|
previous findings in our laboratory have demonstrated that transplants of adrenal medullary tissue into the spinal subarachnoid space can alleviate pain most likely via sustained local release of painreducing neuroactive substances from the Tx chromaffin cells the success of this work in animal models has led to preliminary clinical trials with promising results however before largescale clinical studies are undertaken numerous issues should be resolved many of which can be readily addressed initially in the laboratory one of these is the amount of donor adrenal medullary tissue necessary to produce LT antinociception although tissue from two adrenal glands has generally been used it is unknown whether less would be equally ERP or more could increase analgesic potency to address this various amounts of adrenal medullary tissue ranging from one to ten donor glands were used results showed lowered antinociceptive benefits when only one adrenal medulla was used but only small and shortlived increases when donor material was increased substantially in addition assays of catecholamine and metenkephalin release in the host spinal csf revealed only slight further increases following the transplantation of more than glands these results indicate that a small amount of adrenal medullary tissue is necessary and sufficient to produce sustained antinociception and suggest that higher amounts may result in tolerance OD or feedback inhibition another important issue is the ability to retain antinociceptive potency if donor tissue is maintained in SC prior to transplantation since the coordination of donor harvesting and recipient availability is often difficult to address this donor adrenal medullary tissue was maintained in explant culture for various periods following dissection results indicated that adrenal medullary tissue can be maintained in SC for up to days prior to transplantation without decrement in antinociceptive potency and that a period of days results in improved graft viability results of this T0 indicate that some of the critical issues for successful neural transplantation outcomes can be initially addressed in preclinical studies
|
MEDAL
|
we introduce a new method for preparing subpopulations of guinea pig megakaryocytes mk mk partially purified by a density gradient were separated according to size by sedimentation starting as a monolayer in an albumin gradient at unit gravity twentytwo fractions were collected cells were cytocentrifuged ploidy was assessed by MD and small mk were identified with antivon willebrand factor vwf immunoglobulin immaturity was assessed by uptake of h thymidine and synthesis of proteoglycans from s sulfate about of cells in fractions through were mk of which were viable fractions containing the largest cells were composed of stage iii and iv mk fractions with the smallest cells contained up to stage i and ii mk six mk classes were isolated immature cells both stage i and ii cells at either the n n or n ploidy class mature cells both stage iii and iv cells at either the n n or n ploidy class the fractions were pooled into three CG a of mk in group fractions through were immature and group was composed of of n and n mature classes b of mk in group fractions through were immature and group was composed of n mature n immature and n mature classes of mk in group fractions through were immature and group contained n immature n immature and mature n classes the mean protein content of the three CG was and mg mk respectively nine percent of cells in group but no cells in group took up large amounts of h thymidine the synthesis of highmolecularweight highmolwt proteoglycans in group and synthesis of lower mol wt proteoglycans in groups and provided further evidence for differences in mk maturity thus the method can isolate mk subpopulations that are viable and can be used to investigate the PSA characteristics of mk at different phases of maturation
|
MEDAL
|
the use of the visual evoked potential vep was investigated in terms of its ability to accurately reflect the behaviorally determined extent of the visual field in nonverbal subjects two major adult groups of cats were studied normals and those with neonatal section of the CC at postnatal days of age a lightemitting diode was used as the visual stimulus for both CG the vep determined monocular VF extent closely matched the behaviorally determined field extent elberger this indicates that the vep method is accurate and that it is sensitive enough to detect differences between different populations of subjects
|
MEDAL
|
the prognostic relevance of additional cytogenetic findings at diagnosis of chronic ML cml is unclear the impact of additional cytogenetic findings at diagnosis on time to complete cytogenetic ccr and L1 molecular remission mmr and PFS pfs and overall survival os was analyzed using data from philadelphia chromosomepositive ph cml patients randomized to the german cml study iv at diagnosis of patients had standard tqq only patients had variant tv and additional cytogenetic aberrations acas of these patients lacked the y chromosome y and patients had acas except y of these were major route second philadelphia ph chromosome trisomy isochromosome q or trisomy and minor route all other acas after a median observation time of years for patients with t tv y minor and majorroute acas the year pfs was and and the year os was and respectively in patients with majorroute acas the times to ccr and mmr were longer and pfs and os were shorter p than in patients with standard t we conclude that majorroute acas at diagnosis are associated with a negative impact on survival and signify progression to the accelerated phase and BC
|
MEDAL
|
the prognosis of malignant pleural mesothelioma is poor with a median survival time from diagnosis of to months at present there is no standardized treatment of this neoplasia between july and january patients with malignant pleural mesothelioma were enrolled in our T0 the characteristics of patients were men and women median age years range years stage according to butchart patients stage i stage ii stage iii and stage iv cytologic diagnosis in cases and histologic diagnosis in cases the treatment consisted of mitoxantrone mgm intravenous iv or i.pl. ipl methotrexate mgm iv and MMC mgm iv on day and repeated every weeks with MMC in alternate cycles mmm regimen one CR response months of duration and PRs and months of duration were achieved the overall response rate orr was ci patients were stable under this treatment according to the pathologic type orr for the only epithelial type was ci median time to progression was months range the overall median survival time was months range the median survival time of responders significantly differed from that of nonresponders versus months p this treatment produced a considerable clinical benefit with improvement of dyspnea and pain of patients with pleural effusion at the time of diagnosis showed an important reduction in pleural fluid during chemotherapy hematologic toxicity was the main side effect world health organization WHO to iv of neutropenia anemia and thrombocytopenia were observed in and of patients respectively from the data presented here this regimen can be considered active in the treatment of malignant pleural mesothelioma
|
MEDAL
|
previous studies demonstrated that injecting mice with the cytokine interleukin il could significantly suppress the growth of a number of tumors including mu b melanoma in this report the persistence of the antitumor effects of il is investigated the ip i.t. of il microg on days and was found to significantly suppress the growth of sc inoculated b melanoma for up to weeks after the last i.t. of il interestingly the il SS level days after the last i.t. of il was significantly elevated in tumorbearing mice compared with that of iltreated normal mice the in vivo depletion of either cd or cd t cells abrogated the antitumor activity of il and diminished the apparent autocrine stimulation of il PR seen after il treatment resection of the tumordraining lymph nodes lns but not of the SP abrogated the antitumor effect of il treatment as well as the elevation of SS il expression of mrna encoding il as well as cd ligand cdl was detected in the tumordraining lns but not in the SP of TB mice after il treatment furthermore the antitumor activity observed T3 il treatment was diminished by the in vivo administration of either antiil or anticdl monoclonal antibodies collectively these results suggest that the endogenous production of il resulting from the cdcdl interaction between antigenpresenting cells and cd t cells in the tumordraining lns may play a role in the persistence of the antitumor effects seen T3 il treatment
|
MEDAL
|
the purpose of this T0 was to determine the safety and pharmacokinetics of lamivudine tc a nucleoside analog that has shown potent in vitro and recent in vivo activity against HIV virus sixteen human immunodeficiency virusinfected patients six with normal renal function creatinine clearance clcr or mlmin four with moderate renal impairment clcr to mlmin and six with severe renal impairment clcr mlmin were enrolled in the T0 T3 an overnight fast patients were administered mg of tc orally blood was obtained before tc administration and and h afterward timed urine collections were performed for patients able to produce urine SS and urine were assayed for tc by RP highperformance HPLC-UV pharmacokinetic parameters were calculated by using standard noncompartmental techniques the peak concentration of tc increased with decreasing renal function GMs were and ngml for patients with normal renal function moderate RI and severe renal impairment respectively the terminal halflife also increased with decreasing renal function geometric means were and h for patients with normal renal CF moderate renal impairment and severe renal impairment respectively both oral and renal clearances were linearly correlated with clcr a mg dose of tc was well tolerated by all three patient groups the pharmacokinetics of tc is profoundly affected by impaired renal CF dosage adjustment by either dose reduction or lengthening of the dosing interval is warranted
|
MEDAL
|
sc nexohexahydrohpyrrolizineylmethylmethoxy aminochlorobenzamide hcl a new BA gastrointestinal prokinetic compound was investigated to determine its ability to stimulate gastrointestinal motility in vivo and whether these actions could be mediated by agonist activity at the putative hydroxytryptamine ht receptor in conscious fasted dogs with CS gauge transducers and myoelectrodes sc disrupted gastric and small intestinal migrating motility complex cycling for more than hr it stimulated gastric antral contractile and intestinal ME spike burst MICs during the normally quiescent phase i of the migrating motility complex at doses as low as and mgkg iv respectively in a canine model of gastroparesis sc reversed completely alpha adrenergically DGE of a solid meal with an ed value of mgkg i.g. and partially reversed delayed emptying of a liquid meal sc like ht cisapride and renzapride acted as an agonist ec x m at the putative ht receptor in rat esophageal tunica muscularis mucosae by relaxing carbacholinduced contractions sc was a partial agonist at ht receptors but also blocked high affinity htmediated responses to ht m to x m in guinea pig ileum with a pa value of abstract truncated at words
|
MEDAL
|
anticonvulsant hypersensitivity syndrome ahs is an acute lifethreatening idiosyncratic drug reaction seen within weeks C2 of an aromatic antiepileptic drug the authors present the case of a monthold boy who developed prolonged fever a generalised pruritic rash and eosinophilia within weeks after starting treatment with phenobarbital for complicated febrile seizures he gradually fulfilled the diagnostic criteria for classical kawasaki disease kd although the rash and the subsequent desquamation were atypical he did not defervesce quickly with administration of CSs and intravenous γglobulin and he had only two suggestive cardiac features of kdthat is perivascular echogenicity of the coronary arteries and a small pericardial effusion other conditions considered in the differential diagnosis were excluded by appropriate extensive serological and microbiological studies he recovered fully this report shows that drugs such as phenobarbital may be responsible for febrile exanthematous illnesses that closely mimic kd
|
MEDAL
|
we previously reported that the freely moving F344 rat provides a useful model to demonstrate the progressive impairment of EB conditioning associated with aging however because the youngest f rats only performed at of maximum we ran the same experiment with hybrid rats and discovered most ie those age months learned rapidly and exhibited conditioned responses on greater than of trials by the end of two training sessions in contrast the aged rats months exhibited significantly fewer crs on all four training days however unlike all ages of f rats months which were run in our last study these aged hybrid rats exhibited considerable improvement with extra training these data indicate clear differences in the rate of learning between the two strains and suggest that even young f rats may have deficits in the neural circuits which mediate EB conditioning other anecdotal findings on differences between the two strains are noted
|
MEDAL
|
the neurotoxic actions of the hiv protease inhibitors APV apv and lopinavir lpv were investigated
|
MEDAL
|
the T0 was designed to investigate whether glucocorticoid hormones participate in gastroprotective effects of ICM preconditioning in rats with normal and deficient prostaglandin production to estimate the role of GCs adrenalectomy followed by AGA corticosterone replacement and inhibition of corticosterone synthesis by metyrapone were used prostaglandin synthesis was inhibited by indomethacin in control rats gastric ischemic preconditioning a h ischemiareperfusion induced plasma corticosterone rise and att gastric injury caused by h ischemiareperfusion the gastroprotective effect of ICM preconditioning was prevented by ADX as well as metyrapone i.c.v. in both groups in the rats with normal and deficient prostaglandin production acute corticosterone replacement to ADX during ICM preconditioning restored the protective effect of ICM preconditioning on the GM even in the rats with inhibited prostaglandin synthesis thus the glucocorticoid hormones were shown to contribute to gastroprotective effect of ischemic preconditioning in the rats with normal and deficient prostaglandin production
|
MEDAL
|
ALS als is a rapidly fatal degenerative neurological disease categorized among motor neuron diseases in the literature the incidence of als varies between and per inhabitants a T0 using the CR method multiple records system analysis was undertaken in limousin france ascertaining all patients having onset of definite or probable als during the period three information sources able to identify these new als cases were selected the first source was a computerized database of the neurology department of the UH of limoges the second source consisted of the neurologists of the limousin region and neighboring provinces countysized regions the third source grouped the hospitals of the limousin region and neighboring provinces countysized regions during this period new cases of als were seen corresponding to an observed mean annual incidence of per inhabitants T3 standardization for age the annual incidence was per inhabitants the number of new cases estimated by the capturerecapture method was corresponding to an estimated mean annual incidence of for inhabitants hence statistical modeling utilizing partially overlapping information sources permitted a more exhaustive compendium of the new cases of als and may be a truer reflection of actual disease incidence than has been previously reported
|
MEDAL
|
although MAC mac is usually easy to diagnose by transthoracic echocardiography we experienced a rare case with mac which looked like an intracardiac RT the patient who had been on chronic hemodialysis for years was admitted to our hospital because of dyspnea TTE showed a mass with severe calcification on the anterior mitral annulus and mean mitral gradient of mm hg because of the suspicion of the intracardiac calcified RT that restricted mitral valve motion causing mitral one she underwent resection of the mass and mitral VR pathological findings showed that the mass had a calcified envelope containing liquefied necrotic eosinophilic material with lympocytic infiltrate inside consistent with mac we should consider a possibility of mac when we see a severe calcified mass attached to the mitral annulus in a patient on longterm hemodialysis
|
MEDAL
|
locally aggressive benign lesions of the jaws such as odontogenic keratocysts and ameloblastomas require CR excision in view of the high incidence of recurrence after incomplete surgical removal because of the limitations of conventional radiology as the sole technique for determining the extent of these lesions the use of mtechnetiumlabeled boneimaging agents is suggested this method of defining the location of surgical margins is based on the agents sensitivity as an indicator of subtle changes in bone metabolism a case of an unusually large recurrent odontogenic keratocyst is presented in which the planning of the surgical procedure was predicated on the results of a BS of the jaws in addition to conventional radiology this diagnostic procedure especially when used in conjunction with CR appears to be of considerable value in defining the extent of a variety of oralmaxillofacial bony lesions
|
MEDAL
|
we recently identified dpcsmad as a candidate RT suppressor gene mutated or lost in one half of pancreatic carcinomas and in a subset of colon and biliary tract carcinomas dpc plays a key role in signal transduction of the tgfbeta superfamily of molecules and inactivation of tgfbeta mediated growth inhibition is supposed to be the driving force for dpc inactivation in human PT however dpc mediated RT suppression by reconstitution of defective cells has not yet been reported here we show suppression of tumorigenicity in nude mice by stable reexpression of dpc in sw Caco-2 cells in vitro growth of dpctransfected cells was not affected and resistance towards tgfbeta mediated growth inhibition was retained instead cells exhibited morphological alterations and adhesion and spreading were accelerated these phenotypic changes were associated with reduced expression C2 of the endogenous urokinasetype plasminogen activator upa and plasminogenactivatorinhibitor pai genes the products of which are implicated in the control of cell adhesion and invasion in patients high expression C2 of upa and pai correlate with poor prognosis thus reduced expression of upa and pai is consistent with suppression of tumorigenicity in dpc reconstituted cells these results demonstrate dpcs tumor suppressive CF and suggest a potential role for dpc as a modulator of cell adhesion and invasion
|
MEDAL
|
lactoferrin lf and myeloperoxidase mpo are glycoproteins synthesized in early myeloid cells promyelocytes myelocytes and stored in LG of polymorphonuclear neutrophilic granulocytes both proteins are involved in the host inflammatory response and lf has been found to have myelosuppressive activity in vivo and in vitro little is known however about the regulation of their production we investigated the stability of lf and mpo mrna and the effects of purified recombinant human tnfalpha on lf and mpo levels in normal human bone marrow LD human bone marrow cells were cultured in the presence or absence of actinomycin d microgramsml or tnfalpha uml lf and mpo rna C2 were analyzed by northern blots using respectively a bp insert from the plasmid phl and a kb insert from the plasmid pmpo as probes it was found that lf mrna is a fairly SD molecule with a halflife of between and h whereas mpo is less stable with a halflife of between and h TNF decreases both lf and mpo mrna C2 an effect seen by h with mpo mrna and h with lf mrna nuclear runon assays revealed that tnf decreases transcription of the lf gene by and the mpo gene by and the suppressive effect of tnfalpha on lf and mpo mrna levels is not due to cell killing or selective differentiation and is reversible
|
MEDAL
|
the results of this analysis have demonstrated that PTC carcinoma of the TG rarely causes the death of the patient BL subtotal lobectomies partial lobectomies and probably total lobectomy combined with contralateral partial lobectomy should not be performed in the treatment of this disease the incidence of local recurrence is higher T3 these procedures and any subsequent operative procedure is complicated by scarring secondary to the initial procedure total lobectomy is the procedure of choice for patients with disease confined to one lobe on palpation total Tx should only be performed for palpable disease in both lobes excision of a grossly normal CL lobe solely to obtain microscopic tumor which rarely becomes clinically apparent is not worth the inherent risk of bilateral recurrent palsy of the nerve or permanent HP cervical metastasis of the CL NO which are detected either synchronously or during subsequent followup examination can almost always be resected by some type of modified neck dissection a radical ND with the resection of the jugular vein and the SCM is rarely if ever necessary the administration of thyroid hormone as a surgical adjuvant may not be of any benefit and its routine use is probably not necessary
|
MEDAL
|
the ultrastructural features of three granulosa cell tumors are presented the neoplastic nonluteinized GCs are characterized at submicroscopical level by severely indented nuclei with prominent nucleoli sparse to moderately developed predominantly GER scanty lipids and lysosomes small mitochondria with lamellar cristae and abundant intracytoplasmic filamentous material the luteinized cells display a strongly developed tubular agranular endoplasmic reticulum and mitochondria with tubular cristae these findings are compared with those of previous reports and discussed in relation to the wellknown hormonal activity of these tumors
|
MEDAL
|
melanosomes are lysosomerelated organelles within which melanin pigments are synthesized and stored in melanocytes and RPE cells early ultrastructural studies of pigment cells revealed that melanosomes consist of a complex series of organelles more recently these structures have been correlated with cargo constituents by studying the fate of melanosomal and endosomal cargo in melanocytic cells the effects of diseaserelated mutations on melanosomal morphology and the genes affected by these mutations we are beginning to gain novel insights into the biogenesis of these complex organelles and their relationship to the endocytic pathway these insights demonstrate how specialized cells integrate unique and ubiquitous molecular mechanisms in subverting the endosomal system to generate celltype TPS structures and their associated functions further dissection of the melanosomal system will likely shed light not only on the biogenesis of lysosomerelated organelles but also on general aspects of vesicular transport in the endosomal system
|
MEDAL
|
nippostrongylus brasiliensis a small nematode parasite of the gastrointestinal tract of rodents secretes an enzyme that cleaves the proinflammatory molecule plateletactivating factor to its inactive lysoform the enzyme activity of cadependent and does not exhibit interfacial activation it does not require the addition of reducing agents for Vmax and is not inhibited by thiolactive reagents sensitivity of inhibitors suggests the involvement of serine and histidine residues in the CEA as described for other plateletactivating factor acetylhydrolases it cannot cleave nor is it inhibited by longchain diacyl phospholipids that are typical substrates for phospholipases a the purified enzyme was resolved by sdspage as a heterodimer composed of two protein subunits with apparent M(r) of and kda the properties of the nematode enzyme thus differ from those described for the mammalian enzymes but are more closely related to those of an acetylhydrolase than a phospholipase
|
MEDAL
|
the triterpene alcohol constituents of the nonsaponifiable lipids of two theaceae seed oils sasanqua and camellia oils and two gramineae seed oils wheat germ and rice bran oils were investigated this led to the isolation and characterization of one acyclic and eight incompletely cyclized triterpene alcohols they are camelliol a camelliol b camelliol c achilleol a helianol isohelianol sasanquol graminol a r rsecoabeoalphabetaal phapodioda trienol and zzzeefarnesylfarnesol two other EDC isolated were characterized as zzeegeranylfarnesol a sesterterpene alcohol and phytol a diterpene alcohol graminol a and zzeegeranylfarnesol are considered to be new NK products
|
MEDAL
|
IVUS imaging on the endarterectomized coronary arteries in two patients are reported with a review of the literature case a yearold male and case a yearold male underwent endarterectomy of the right PD pd av combined with coronary arterial bypass grafting coronary arteriography cag and intravascular ultrasound imaging ivus were performed one month after operation cag revealed bypass grafts and endarterectomized CAs to be patent together and ivus revealed endarterectomized coronary arteries had two layers outer SL to be hyperechoic and inner layer to be heterogenously echoic in both cases the histological examination of specimens suggests this heterogenously echoic area represents media thrombus and irregularly proliferated intima therefore ACT after endarterectomy should be strictly performed
|
MEDAL
|
we report the case of a ventricular septal aneurysm in a patient with a previous inferior myocardial infarction twodimensional echocardiography demonstrated a cystic cavity in the muscular SP with a small communication into the left ventricle no evidence of lefttoright shunt was detected with doppler echocardiography or during left VG
|
MEDAL
|
this paper describes the separation of the main component micronomicin from its related substances using a new established LC method with pulsed EC detection lcped the mobile phase consists of volume of acetonitrile and volumes of an aqueous solution containing vv trifluoroacetic acid vv pentafluoropropionic acid and vv of sodium XTT the ph of the aqueous solution is adjusted to with m naoh the influence of the different chromatographic parameters on the separation was investigated a quadruplepotential waveform was used as detection waveform m naoh was added post column at a flow rate of mlmin to raise the ph of PCD to at least the lod and loq of micronomicin are μgml ng injected and μgml ng injected respectively the linearity of MCR ranges from to μgml with a correlation coefficient of intraday rsd and interday rsd of MCR are and respectively this method proved to be robust and is also applicable to a wider number of c columns a number of commercial samples of MCR sulfate were analyzed using this method and peaks can be separated from the main component and from each other in one sample seven peaks could be identified using REF substances the chemical structure of two unknown impurities could be characterized by lcms based on comparison of their fragmentation patterns with those of available REF substances
|
MEDAL
|
a LIP an upper inflection or deflection point and respiratory system compliance can be estimated from an inspiratory static pressurevolume spv curve of the respiratory system such data are often used to guide selection of positive endexpiratory pressure peeptidal volume combinations dynamic pressurevolume dpv curves obtained during tidal ventilation are effortlessly displayed on modern mechanical ventilator monitors and bear a theoretical but unproven relationship to the more laborintensive spv curves
|
MEDAL
|
in ST the relationship between dna copy number and global expression over C1 chromosomal regions has not been systematically explored we used a gene expression array analysis of head and neck squamous cell carcinoma and normal oral mucosa and annotated mRNA levels to TPS chromosomal loci expression alterations correlated with reported data using comparative ge were already undertaking a range of public health and health education activities but much of the work was opportunistic district nurses perceived themselves as well placed to undertake locally based health action because of their profile in the local communities together with local IQ however ineffective referral criteria were considered to be the most prohibitive barrier against making a greater contribution to health improvement
|
MEDAL
|
this study is focused on the volatile oils from the fruiting bodies of pleurotus salmoneostramineus ps and p sajorcaju psc which was extracted by hydrodistillation hd and solventassisted flavor evaporation safe methods the oils are analyzed by gas chromatographymass spectrometry gcms gcolfactometry gco and aroma extract dilution analysis aeda a total of and components were identified in ps hd and safe and psc hd and safe representing about and of the oils respectively regarding the aromaactive components and components were identified in ps hd and safe and psc hd and safe respectively by the gco analyses the results of the sniffing test odor activity value oav and flavor dilution fd factor indicate that octenol and octanone are the main aromaactive components of ps oils on the other hands methional and octenol were estimated as the main aromaactive components of psc oils
|
MEDAL
|
postocclusion reactive hyperemia and isolated leg exercise were evaluated as techniques for PET evaluation of LE arterial insufficiency by comparing them with TM exercise sixty patients with claudication and six normal subjects were evaluated changes in doppler ankle pressures were measured T3 each form of stress with substantial occlusive disease there is a drop in pressure with gradual recovery postocclusion reactive hyperemia and isolated leg exercise are abnormal if the pressure does not return to resting C2 within two T2 there was good rho between the three methods postocclusion reactive hyperemia avoids the problems of patient cooperation inherent in the other two methods postocclusion reactive hyperemia should probably replace treadmill exercise as the first method of stress testing lower extremities
|
MEDAL
|
in mesangial cells mc the response of intracellular ca concentration cai to a contractile agonist is biphasic with a large transient increase in cai followed by a smaller but sustained elevation as ca flows into the cell from the ECF it has been postulated that membrane depolarization precedes opening of ca channels in the plasmalemmal membrane however a role for voltagegated ICa vgcc in human mc has been controversial and their existence has not been verified with singlechannel analysis we used fura fluorescence and patchclamp techniques to determine the properties of the ca entry pathway responsible for the sustained response of cai in human mc we found that ang ii at nm nm and microm increased cai to sustained levels of and respectively above baseline the sustained response to microm ang ii was attenuated by diltiazem and was reduced to a value less than baseline in the absence of external ca none of the peak responses due to release of intracellular stores of ca were affected by removal of external ca or addition of diltiazem upon elevating the extracellular k from mm to mm cai reached a sustained level of greater than baseline this effect of HK was attenuated by either ca removal or addition of diltiazem in the presence of or mm k the dihydropyridine agonist bay k microm and microm initiated sustained cai responses averaging and respectively greater than baseline with nm ca in the external solution bay k did not significantly affect cai in separate patchclamp experiments bariumselective channels were found in cellattached patches with mm bacl and microm bay k in the pipette solution the singlechannel conductance was ps and the open probability increased steeply at MPs between mv and mv it is concluded that human glomerular mc contain dihydropyridinesensitive ca channels responsible for the voltageregulated entry of ca into the cell during an agonistinduced contraction
|
MEDAL
|
the NT of the AC AT and one effects of DS ds a potential antithrombotic agent was investigated PS ps and hexadimethrine bromide PB which reverse the anticoagulant effect of heparin also neutralized ds in vitro in human plasma PB was approximately times more active on a weight basis than ps for neutralizing ds micrograms PB inhibits microgram ds intravenous administration of polybrene to rabbits pretreated with ds in a weight ratio immediately neutralized of ds and this effect was SD with time in contrast ps in a weight ratio of ps to ds only neutralized of ds injected when plasma ds concentrations were maintained by continuous infusion between and microgramsml a bolus of PB mgkg induced an immediate drop of about microgramsml but initial values of ds were recovered within min ps was again much less ERP than PB for NT ds the bleeding effect of ds and its correction by PB was studied by using the rat tail transection MM very C1 doses of ds greater than mgkg were required to get a modest prolongation of BT the injection of equivalent doses of PB in animals pretreated by ds induced a strong bleeding effect associated with a drop in PLT and leukocyte counts animal models are thus inappropriate for investigating the correction of dsinduced bleeding because high doses of both ds and neutralizing agents are required in these models our results indicate that provided the doses of neutralizing agents remain below their established levels of toxicity in man ds could if necessary be neutralized CR by polybrene and partially by ps
|
MEDAL
|
benign paroxysmal positional vertigo bppv is a common vestibular disorder the recent development of diagnostic tools allowed clarification of its pathology dislodgement of the UT otoconia is the basis of bppv which leads to OD of canalolithiasis or cupulolithiasis positional nystagmus test is a most essential bed side test bppv may involve multiple canals and utricle thus showing complicated nystagmus DP understanding the physiology of semicircular canals and vestibuloocular reflex allows proper diagnosis of a lesion side and types of bppv based upon the basic and clinical findings a lesionspecific PCS therapy had been developed which turned out to be very effective nystagmus pattern can also be an index of the treatment outcome
|
MEDAL
|
we have found that if core regions crucial for class ii IB are incorporated in multiple copies in the same peptide molecule reiterative motifs marked enhancement of the IB capacity occurs isotype specificity iad vs ied binding capacities is retained in all three antigenic determinants so far analyzed lambda rep ova and hen egg lysozyme the mechanism involved in such an effect is not clear but experiments involving introduction of a peptide spacer between two repeated core regions do not support the notion that the effect is mediated by crosslinking of more than one mhc molecule favoring the possibility that conformational effects or distinct subsites of interaction on the mhc molecule may be involved based on reiterative structures a peptide molecule composed of only two different amino acids ala and his has been produced that still retains a very high binding affinity an iradiolabeled form of this peptide has been used to demonstrate that the high binding detected is mediated by the same IB site involved in the interaction of iad and ova inhibition of ag presentation studies further supports the immunologic relevance of the phenomena observed finally we observed naturally occurring clustered IB sites in proximity of immunodominant protein regions raising the possibility that the phenomenon might have a physiologic counterpart
|
MEDAL
|
the aim of the study was to evaluate the effect of acetaminophen apap andor trichloroethylene tri on the CL cytochrome pdependent monooxygenase system cype and cypa two important p isoforms and liver glutathione gsh content in rats rats were given three different doses of apap and mgkg b and then the abovementioned parameters were measured for h the lowest apap dose produced small changes in the cytochrome p content of CL at mgkg apap increased the cytochrome p content to of the control the inductive effect was seen at mgkg dose but at h and later or activity was the highest T3 the lowest dose of apap while after the highest dose it was equal to the control value tri increased both the cytochrome p content and the nadphcytochrome p reductase activity when tri was combined with apap both these parameters increased in the first hours of observation but they returned to the control values at h when apap was given at mgkg gsh levels decreased to of the control at h and returned to the control values at h the higher doses of apap decreased gsh levels more than the lowest dose but after h gsh levels did not differ from those of the control when tri was given at mgkg the gsh levels decreased to of the control at h and then they increased gradually and tended to exceed the control values at h the effect of tri combined with apap on the level of gsh was virtually the same as that of apap alone given at mgkg
|
MEDAL
|
primary aldosteronism is usually diagnosed after a battery of biochemical tests and adrenal imaging if an adrenal tumour conns syndrome is identified patients need surgery however bilateral adrenal hyperplasia is treated medically till we diagnosed conns syndrome using simple biochemical tests and a ct scan of the adrenal glands aldosterone and plasma renin activity assay became available in our institution in and were used subsequently in the work up of patients suspected to have conns syndrome we analysed our data to determine whether simple tests such as SS and urinary potassium values combined with ct imaging of the adrenal PG are adequate to arrive at a diagnosis in these patients
|
MEDAL
|
the role of CAs insulin and thyroid hormones on PP thermogenesis was determined in dogs o consumption vo and respiratory quotient rq were continuously monitored h before and h T3 a kcal meal under the three following exp conditions corresponding to normal feeding sham feeding with food deflected into an esophageal pouch and tube feeding i.g. a first phase cephalic phase lasting at least min was observed during both normal and sham FF whereas a second phase digestive phase occurred in both normal and tube feeding increases of for norepinephrine ne were found during the cephalic phase in both normal and SF epinephrine e increased by and during this EP in normal and sham FF respectively no change in CA was found in the tubefeeding experiment large increases of and microuml of insulin occurred early T3 the meal in both normal and SF whereas insulin increased only T3 min in tube FF thyroxine and triiodothyronine were not changed with any FF experiment insulin NC was positively correlated with ne and e changes rather than with glucose NC during the cephalic phase after feeding the results suggest an important role of CAs in the increased postprandial thermogenesis during the cephalic phase with a possible modulating effect of insulin
|
MEDAL
|
tau and safety of unasyn a combination of SBT and ampicillin was studied in the treatment of patients with infections of the urogenital organs the drug was IM intravenously and orally the treatment course averaged days the dose of the drug for the intravenous and intramuscular administration was g times a day and that for the oral administration was g times a day the strains of GPC escherichia coli and proteus spp proved to be highly CS to the drug and per cent of the CS strains respectively per cent of the strains showed betalactamase activity that was most frequently detected in proteus spp and staphylococcus spp the maximum concentrations of ampicillin and sulbactam determined by liquid chromatography were respectively and microgramsml in the blood and and microgramsml in the urine a satisfactory bacteriological effect of the treatment was observed in per cent of the cases a complete elimination of the initial pathogens was stated in per cent of the cases no SE were recorded
|
MEDAL
|
lower limb joint loadings are increased during sloped walking compared to level walking and muscle forces are major contributors to lower limb joint forces therefore the aim of this T0 was to analyze lower limb muscle forces during sloped walking at different inclinations eighteen healthy male participants ±y ±m ±kg walked at a preset speed of ms on a ramp at the inclinations of ° ±° ±° and ±° kinematic data were captured with a motion capture system and kinetic data were recorded with two force plates imbedded into the ramp a MSK model was used to compute lower limb muscle forces normalized to body weight and gait cycle duration during downhill walking GM quadriceps soleus peroneus and TA muscle forces increased p⤠compared to level walking while gluteus minimus piriformis adductor iliopsoas hamstrings and gastrocnemii muscle forces decreased p⤠uphill walking decreased gluteus minimus iliopsoas and TA muscle forces p⤠while all other muscle forces increased p⤠except gluteus medius jointmuscleforce waveforms provided information on possible muscle contributions to joint compression forces the most important muscles were gluteus medius for hip forces quadriceps and gastrocnemii for tibiofemoral forces quadriceps for PF forces and triceps surae for ankle forces the contribution of each muscle changed with the inclination during sloped walking compared to level walking the current T0 provided important information on muscle forces during sloped walking that can be useful for rehabilitation and training procedures
|
MEDAL
|
the patient with febrile rash poses a real diagnostic challenge to PC physician we report an original case of febrile macular rash whose etiology was related to sepsis secondary to pelviperitonitis and acute cholecystitis patients PH careful PE paraclinical examinations and favorable outcome allowed to retain the infectious origin of the rash without microbiological confirmation in our study the skin is an excellent marker for infection cutaneous manifestations are the most common signs observed in patients with sepsis at an early stage exanthema is the most common lesion it is due to systemic effects of a microorganism infecting the skin if there are no clinical signs of infection early diagnosis can prevent complications
|
MEDAL
|
environmental exposures filtered through the genetic makeup of each individual alter the transcriptional repertoire in organs central to metabolic homeostasis thereby affecting arterial lipid accumulation inflammation and the OD of coronary i.a. disease cad the primary aim of the stockholm atherosclerosis mRNA stage T0 was to determine whether there are functionally associated genes rather than individual genes important for cad OD to this end twoway clustering was used on transcriptional profiles of liver skeletal muscle and visceral fat n tissue and AS and unaffected arterial wall n tissue isolated from cad patients during PD bypass surgery the first step across all mrna signals n refseqsgenes in each tissue resulted in a total of tissue clusters n genes in the second step performed within tissue and have been linked to synaptic STDP we investigated whether kibra and clstn interactively modulate episodic memory performance n we replicated the beneficial effect of the kibra tallele on EM and discovered that this effect increases with the associative demands of the memory task importantly the memoryenhancing effect of the kibra tallele was boosted by the presence of the clstn callele which positively affected SM performance in some previous studies in contrast the presence of clstn callele led to reduced performance in subjects homozygous for the kibra callele overall these findings suggest that kibra and clstn interactively modulate EM performance and underscore the need for delineating the interactive effects of multiple genes on brain and behavior
|
MEDAL
|
there are limited data on agerelated changes in body composition or skeletal muscle in the horse therefore the purpose of this study was to investigate any differences in muscle myosin heavy chain mhc and BC associated with aging twentythree young years and eight old years unfit standardbred mares were evaluated rump fat thickness was measured using bmode ultrasound and per cent body fat fat was calculated needle muscle biopsies were obtained from right gluteus medius muscle mhc composition was determined via sodium dodecyl sulphatepolyacrylamide gel electrophoresis three mhc isoforms were subsequently identified as type i type iia and type iix and quantified using a scanning and densometric system there were no significant differences p between old and young mares in fat vs fat mass kg vs or body weight kg vs however the old mares had significantly p greater lean body mass than the young mares vs aged mares had significantly p less type i vs and iia vs MF than the young group but more type iix vs the mhc data are consistent with the agerelated changes seen in other species
|
MEDAL
|
it was not until that autistic spectrum disorders were officially recognized as disorders of OD and not of psychoses wing in this article the term autism will be used to refer to all individuals diagnosed across the autistic spectrum including people with asperger syndrome there will be many adults with autism aged over years of age who are not appropriately diagnosed and who are not receiving relevant care trevarthen et al many will have been the victims of a medical MM of treatment as opposed to receiving the necessary education and support this article the second of a twopart series the first article vol dealt with children with autism will explore how contemporary knowledge can help healthcare professionals to support adults with autism in the development of positive selfidentities
|
MEDAL
|
there is an increasing interest in anticonvulsants for the treatment of benzodiazepine withdrawal PS the new anbed shear AS the sediment deposits were formed using three different types of sediment an artificial organic sediment together with a uniformly sized sand were selected as surrogate sewer sediments the deposits in the remaining experiments were real insewer sediments from catchments in the uk dundee and the netherlands loenen during the erosion test total and volatile suspended solids concentration particle size distribution of the eroded sediment and cod and do levels were recorded the bed surface topography was also measured so that the influence of the deposit formation condition on bedforms could also be examined where bed consolidation times were least hours a biologically AS surficial layer was observed to develop at the sedimentwater interface the initial deposit conditions temperature and deposit duration were both found to have a significant impact on the subsequent erosion of the deposit
|
MEDAL
|
lack of knowledge and confusion exists regarding safe and appropriate use of BGM equipment increasing numbers of diabetics and exponential growth in BGM presents increased opportunities for infection transmission between patients diabetics have increased exposure to blood and blood borne pathogens from frequent blood glucose monitoring risk factors have been identified in infectious outbreaks and by analysis of testing practice point of care blood gl meters are frequently contaminated by blood bacterial and viral organisms survive on surfaces and in dried blood instrumentation is shared between patients and is heavily utilized in institutional settings so that serial testing is performed on multiple patients within a short timeframe hand hygiene glove changes and meter disinfection between testing events has been found to be inconsistent time pressure for meter usage competes with proper cleaning and disinfection procedures meter storage Az are frequently contaminated by blood multiuse lancets improperly used for serial patient blood sampling are a source for infection transmission test strips in vials frequently contaminated by bacterial organisms present potential hazard the responsibility of the clinical laboratory is to insure successful implementation of practices that insure patient safety risk reduction strategies include singleuse autodisabling skin puncture devices for blood sampling hand hygiene and glove NC for every testing event ERP meter cleaning and disinfection for every testing event meter use restriction to a single patient safe practices for gl meter storage IC practices to reduce contamination of blood glucose test strips or changes in test strip packaging and test strip dispensing
|
MEDAL
|
research by jacobson on development of a semantic differential for external comparison of conceptual nursing models was reported in the present article critiques the philosophical stance and the research methods presented in the jacobson report methods of analysis and DUE of conceptual nursing models are reviewed and the influences of conceptual nursing models on the structure of the discipline are examined in light of the purposes of the jacobson study issues related to the investigation and use of models in the OD of nursing knowledge are discussed
|
MEDAL
|
SC is characterized by formation of cartilaginous NO possibly with ossification in the synovial membrane of the joints tendons and vagina the description of a case of SC located in the knee joint capsule is presented histologically apart from hyaline cartilage resembling chondroma the Az simulating chondrosarcoma were found the differential diagnosis between synovial chondromatosis and CS is proposed
|
MEDAL
|
interdigitating dendritic cell sarcoma idcs is an extremely rare AT1 of dendritic cell neoplasms and current knowledge on this tumor is limited we herein report a case of an idcs in a yearold man who presented with a right inguinal mass combined with extensive RP pulmonary hepatic renal and bone marrow infiltration because of the advanced stage of the disease we performed five cycles of chemotherapy including cyclophosphamide doxorubicin vincristine and prednisone chop doxorubicin bleomycin vinblastine and dacarbazine abvd and abvd combined with cisplatin and one cycle of radiotherapy the patients inguinal mass became smaller during the treatment but there was no change in the extent of infiltration at the other CS the patient died months T3 the initial diagnosis we also herein review the etiology diagnosis differential diagnosis treatment and prognosis of idcs and analyze the characteristics of idcs in chinese patients
|
MEDAL
|
we evaluated the effect of glycated Al on phenytoin protein binding in elderly age range yrs patients with type ii diabetes mellitus dm under diet management serum was spiked with mgl phenytoin and incubated a serum UF was obtained from each sample for determining total and free phenytoin concentrations glycated hemoglobin was determined by boronateaffinity chromatography and GA was separated from nonglycated fractions with boronateagarose gel glycated hemoglobin in the study group ranged from mean sd and GA ranged from sd we observed no correlation between GA and the percentage of free phenytoin r p the concentration of nonglycated albumin ranged from gdl mean gdl and was calculated from measured total and glycated albumin concentrations a rho between the free fraction of phenytoin and nonglycated albumin was not demonstrated r p in addition a correlation was not observed between total glycated albumin and the free fraction of phenytoin r p we conclude that elderly patients with type ii dm under diet control do not have significant alterations in phenytoin protein binding the use of total serum phenytoin levels therefore appears AGA for determining phenytoin dosages in elderly patients with well controlled type ii dm
|
MEDAL
|
simple and reliable methods have been sought for both predicting and confirming ovulation application of these methods could include management of infertile couples to aid in conception and for increasing the reliability of NFP nfp as a method of birth control with the advent of specific hormone assays serial measurements of estrogens progesterone and metabolites and luteinizing hormone have been the gold standard of monitoring ovarian CF in women however newer and simpler methodologies have been described and are currently either in use or being tested these include the measurement of basal body temperature bbt the evaluation of the volume consistency and electroconductivity of cervicovaginal fluid salivary steroid content and cellular enzymatic activity the use of enzymelinked immunosorbent assays applied to solidphase formats and the investigation of new hormonal molecules as markers of reproductive state and function these new technologies are described herein and their potential for monitoring ovarian function is discussed
|
MEDAL
|
an yearold female with fever edema in the lower extremities and marked eosinophilia was referred to our hospital based on the presence of the philadelphia chromosome she was diagnosed with chronic myeloid leukemia cml although imatinib induced a complete cytogenetic response ccyr cml relapsed after months of starting it a ccyr was achieved again by nilotinib but was lost after about months only transient response occurred to dasatinib and the patient died at relapse neutrophilia was more predominant than eosinophilia we reviewed patients with cml whose eosinophil rate in the peripheral blood was most patients were males with palpable splenomegaly and had cardiac disorders PVD or pleural effusion typically cml causes neutrophilpredominant leukocytosis however a subgroup of cml with marked eosinophilia resembles chronic eosinophilic leukemia or idiopathic hypereosinophilic syndrome
|
MEDAL
|
the transgenic growth retarded tgr rat is the first genetic MM of growth hormone GHD whose growth can be accelerated with exogenous gh secretagogues ghss in this study we have demonstrated that ghsreceptor ghsr mrna expression in the arcuate nucleus of tgr rats was not significantly different to that in wildtype littermates we have confirmed that ghsinduced elevation in body WG was accompanied by acceleration of skeletal growth and that the effects of the ghs ghrp were both dose and patterndependent the growth response with continuous infusion of ghrp was transient accompanied by suppression of gh and corticosterone responses to bolus injection of ghrp this desensitization occurred without downregulation of arcuate ghsr mrna expression but was accompanied by elevated periventricular somatostatin mrna expression in contrast pulsatile hourly infusion of ghrp produced sustained growth and gh responses which were accompanied by suppression of corticosterone responses and elevated arcuate ghreleasing factor grf mrna expression skeletal growth was further accelerated by coinfusion of grf but significant depletion of pituitary gh stores suggested that this growth rate may not be sustainable these experiments confirm the importance of the tgr rat for investigating the growth promoting potential of the ghss in the context of ghdeficient dwarfism and suggest that elevated somatostatin expression may mediate the suppression of the grfgh and hypothalamopituitaryadrenal axes following continuous ghrp treatment
|
MEDAL
|
the role of nutritional supplementation is of increasing interest with regard to ocular disease randomised controlled trials have demonstrated the effectiveness of supplementation for agerelated macular degeneration and formulations are now being developed for use by people with diabetes and DR the aim of this review was to synthesise the evidence for use of nutritional supplementation in type diabetes medline and embase databases were searched using a systematic RPA only doublemasked randomised controlled trials were selected a total of trials were identified as suitable for inclusion the potential role of alphalipoic acid chromium folic acid isoflavones magnesium pycnogenol Se VC vitamin e and zinc in the treatment of type diabetes is discussed the review of trials identifies positive effects of these nutrients on various outcome measures relating to insulin resistance and cardiovascular AF chromium was the most studied supplement accounting for of the trials a majority of the trials found a positive effect of chromium on FPG isoflavones were found to have a positive effect on insulin resistance and cardiovascular outcome measures but only when combined with soy proteins VE is reported to reduce oxidative AS at C2 of mg day or more
|
MEDAL
|
interleukin beta il beta is a potent acth secretagogue which activates the PR of hypothalamic crh direct injections of il beta into the Thy ME me a site which lacks a bloodbrain barrier has been shown to rapidly induce acth secretion therefore the me is a likely site whereby circulating il beta can access the brain to stimulate crh and consequently acth secretion to further evaluate this hypothesis an angular stereotaxic RPA was developed to localize the spread of il beta to the me and to optimally separate the injectate from the hypothalamic paraventricular nucleus pvn another proposed site of il action studies of the diffusion of iil beta nl delivered over s showed that remained within microns of the ventral surface of the hypothalamus and was contained within a radius of microns of the injection site in the sagittal plane additional rats received recombinant human il beta ng in nl into the me intrame plasma acth C2 were significantly elevated by a much lower dose ng p of il beta than that previously reported responses appeared to be dosedependent and acth was maximally stimulated by ng il beta also immunocytochemically labelled crh in the me was markedly depleted after intrame il beta indomethacin an inhibitor of prostaglandin pg synthesis has been shown to block both the induction of crh secretion by il beta from Thy explants as well as the acth response to intravenous il beta thus indomethacin was used to determine whether pgs are mediators of the acth response to il beta delivered into the me the acth response was abolished p when a low dose of indomethacin mgkg iv was po min before intrame il beta ng finally plasma acth was elevated in a dosedependent manner by the intrame administration of pgs the hierarchy of acth responses to pge were csf micrograms p micrograms micrograms p responses to pgf alpha were csf micrograms p micrograms p micrograms since these pgs appear to activate different secondmessenger systems a submaximal dose of each was po alone or in combinationabstract truncated at words
|
MEDAL
|
a coated wire triamtereneselective electrode based on the incorporation of a triamterenetetraphenylborate ionpair in a PVC coating membrane was constructed the influence of membrane composition temperature ph of the test solution and foreign ions on the electrode performance were investigated the electrode showed a nernstian response over a TA concentration range from x to x m at degrees c and was found to be very selective precise and usable within the ph range the standard electrode potentials e degrees were determined at and degrees c and used to calculate the isothermal temperature coefficient de degrees dt of the electrode temperatures higher than degrees c seriously affected the electrode performance the electrode was successfully applied to the potentiometric determination of triamterene hydrochloride both in pure solutions and in pharmaceutical S9
|
MEDAL
|
intracellular ph is known to increase during agonistinduced PLT activation in order to elucidate the role of intracellular alkalinization in PLT activation the effects of nhcl as a tool to induce intracellular alkalinization on ionomycininduced platelet activation were investigated nhcl mm concentrationdependently induced intracellular alkalinization PLT aggregation induced by ionomycin microm was augmented by treatment with nhcl mm ionomycininduced PLT aggregation in the absence of extraplatelet ca which was markedly att compared to that in the presence of extraplatelet ca was also augmented by nhcl nhcl treatment increased the number of large aggregates T3 ionomycin stimulation while it decreased the number of small aggregates both transplasmalemmal ca entry and intracellular ca release induced by ionomycin were increased by treatment with nhcl mm skf microm an inhibitor of ROCs did not affect ionomycininduced ca entry but abolished the effect of nhcl on ca entry thus nhcl augments receptoroperated ICa and intracellular ca release these findings suggest that intracellular alkalinization plays a significant role in agonistinduced platelet activation
|
MEDAL
|
we established hela cell lines that constitutively expressed the fusion f andor haemagglutininneuraminidase hn glycoproteins of human parainfluenza virus type a piva and used them to analyse the roles of these glycoproteins in virusinduced cell F0 no syncytium formation occurred even in hela cells expressing both the f and hn proteins helaafhn cells also no syncytium was found in a mixed SC of cells expressing the f protein helaaf and the hn protein helaahn syncytia were observed in helaaf cells transfected with the hn gene but no syncytium formation was found in helaahn cells transfected with the f gene cocultivation of helaafhn cells with helaaf cells generated C1 polykaryocytes whereas cocultivation with helaahn cells induced no cell fusion infection of helaaf cells with piva generated large syncytia and degenerated nuclei whereas little or no polykaryocytes were found in helaahn cells INF with piva from the above findings the following conclusions were drawn i the expression of both the f and hn proteins in the same cell is necessary for cell F0 ii the expression of the f protein CT enhances susceptibility to cell F0 iii the constitutive expression of the hn protein promotes resistance to paramyxovirusinduced cell F0
|
MEDAL
|
it is an important challenge for bone repair to effectively deliver growth AF and at the same time to prevent and cure inflammation without obvious pathogen resistance we designed a kind of antibacterial peptidemimetic alternating copolymers pmacs to effectively inhibit and kill both GP and gramnegative bacteria the minimum inhibition concentrations mics of the pmacs against e coli and s aureus are μgml which are much lower than that of antibacterial MMP synthesized by other methods such as widely used ringopening polymerization of ncarboxyanhydride furthermore the pmacs can selfassemble into polymer vesicles polymersomes in pure water with low cytotoxicity ic
|
MEDAL
|
the L1 acidsoluble spore proteins assps of Bs were detected by immunoprecipitation of radioactively labeled in vitro and in vivosynthesized proteins assp synthesis in vivo began h after the initiation of sporulation t and reached its maximum rate at t this corresponded to the time of synthesis of mrna that stimulated the maximum rate of assp synthesis in vitro under the set of conditions used in these experiments protease synthesis began near t ALP phosphatase synthesis began at about t and refractile spores were first observed between t and t in vivo and in vitrosynthesized assps comigrated in sodium dodecyl sulfatepolyacrylamide gels their molecular weights were alpha and beta and gamma the average halflife of the assp messages was min when either RFP microgramsml or actinomycin d microgramml was used to inhibit rna synthesis
|
MEDAL
|
fluoride phases that contain the spin d agii ion have recently been predicted to have interesting or unusual magnetochemistry owing to their structural similarity to the d cuii cuprates and the covalence associated with this unusual oxidation state of silver here we present a comprehensive study of structure and magnetism in the layered agii fluoride csagf using magnetic susceptometry inelastic neutron scattering techniques and both xray and neutron powder diffraction we find that this material is well described as a twodimensional ferromagnet in sharp contrast to the hightc cuprates and a previous report in the literature analyses of the structural data show that csagf is orbitally ordered at all temperatures of measurement therefore we suggest that orbital ordering may be the origin of the ferromagnetism we observe in this material
|
MEDAL
|
assessment of the implications of clinical and noninvasive variables including the results of SAECG was performed or weeks after myocardial infarction in patients with nonsustained IVT vt who underwent programmed VVI stimulation to guide antiarrhythmic therapy the clinical and noninvasive parameters assessed included ages left ventricular ejection fractions sites of infarction presence of akinetic or dyskinetic left VVI segments PH of syncope PH of PD bypass surgery and presence or absence of late potentials from SAECG other than the presence of LPs no clinical or noninvasive parameters identified such persons with a significantly higher likelihood of inducible vt when assessed as positive if or more variables were abnormal of patients with versus of without inducible vt had late potentials p with more stringent criteria required defined as prolongation of the qrs vector complex duration and low rootmeansquare voltage of the terminal ms of the vector complex of patients with and of without inducible vt had LPs recorded p thus the SAE may enable identification of persons with nonsustained vt after myocardial infarction who are most likely to have vt induced at programmed ventricular stimulation
|
MEDAL
|
voltagedependent sodium ina and calcium ica currents in small microm SN from AD rat trigeminal root ganglia were characterized with a standard whole cell patchclamp technique two types of ina showing different sensitivity to tetrodotoxin ttx were recorded which showed marked differences in their activating and inactivating time courses the activation and the steadystate inactivation kinetics of ttxresistant ina were more depolarized by about and mv respectively than those of TTX-S ina voltagedependent ica was recorded under the condition that suppressed sodium and potassium currents with mm ca as a charge carrier depolarizing step pulses from a holding potential of mv evoked two distinct inward ica lowvoltage activated lva and highvoltage G1 hva ica lva ica was first observed at to mv and reached a peak at about mv amiloride mm suppressed approximately of the lva ica whereas approximately of hva ica was inhibited by the same concentration of the amiloride lva ica was far less affected by the presence of external cd or the replacement of ca by ba than hva ica the omegaconotoxin gvia omegacgtx an ntype ica blocker suppressed approximately of the whole cell hva ica at the concentration of microm the omegacgtxresistant hva ica was CS to nifedipine microm a dihydropyridine dhp calcium channel antagonist which produced an additional blockade by approximately of the drugfree control approximately of the omegacgtxresistant ica the combination of microm nifedipine and microm omegacgtx left approximately of the drugfree control ica unblocked the dhp agonist sbayk microm shifted the activation of the hva ica to more negative potentials and increased its maximal amplitude additionally sbayk caused the appearance of a slowed component of the tail current these results clearly demonstrate that the presence of two types of sodium channels ttx CS and resistant and three types of calcium channels t l and n type in the smallsized adult rat TG ganglion SN
|
MEDAL
|
the aim of the study was to investigate the elicitation potential of perfumes from commonly sold lowerprice cosmetic products of the perfumes were from stayon cosmetics and were from washoff cosmetics each perfume was tested in consecutive eczema patients who also were tested with the european standard patch test series reacted to or more of the washoff product perfumes and to or more of the stayon product perfumes concordant positive reactions between the fragrance mix and the product perfumes were found in of positive reactions to the stayon product perfumes and in of the reactions to the washoff product perfumes compared to the FM CT only additional case of contact allergy to the product perfumes was detected by balsam of peru chemical analysis revealed that between and of the chemicallydefined constituents of the fragrance mix were present in all of the product perfumes geraniol was found in of the perfumes and was most often detected the concentration of the target fragrance materials ranged from wv it is concluded that the allergenic constituents of the fragrance mix are impossible to avoid if perfumed cosmetics are used furthermore patients suspected of perfume allergy need to be tested with their own perfumed products as far from all cases of perfume allergy are detected by the fragrance mix andor balsam of peru in the EU standard patch test series
|
MEDAL
|
almost all pacemakers currently implanted in patients in the united states are now capable of some degree of noninvasive programmability although programmability seems to be a very useful feature and allows flexibility in the patients pacemaker system the use of programmability in the late followup period has not been well substantiated we compare the use of programmability in the early and late postoperative periods in consecutive patients who received a multiparameter programmable pulse generator programmability is definitely a desirable feature particularly for alterations in rate PW and sensitivity
|
MEDAL
|
an investigational live influenza virus vaccine flumist contains three coldadapted hn hn and b influenza viruses the vaccine viruses are reassortants in which the hemagglutinin ha and neuraminidase na genes are derived from the circulating wildtype viruses and the remaining six genes are derived from the coldadapted master donor strains the six genes from the coldadapted master donor strains ensure the attenuation and the ha and na genes from the wildtype viruses confer the ability to induce protective immunity against contemporary influenza strains the genotypic stability of this vaccine was studied by employing clinical samples collected during an tau trial viruses present in the nasal and throat swab specimens and in S9 T3 culturing the specimens were detected and subtyped by multiplex reverse transcriptase rtpcr CR genotypes of these detected viruses were determined by a combination of rtpcr and restriction fragment length polymorphism multiplex rtpcr and FL singlestrand conformation polymorphism and nucleic acid sequencing analysis the flumist vaccine appeared to be genotypically SD T3 replication in the human host all viruses detected during the week PV period were shed vaccine viruses and had maintained the genotype
|
MEDAL
|
seronegative SpA may be complicated by a variety of cardiac lesions including aortitis we describe a patient with reiters syndrome who presented with angina and new ON HR murmurs cardiac catheterization revealed MI and AI our patient underwent bypass surgery with aortic valve replacement severe narrowing of the coronary ostia was noted and aortitis was documented by pathologic examination
|
MEDAL
|
in a yearold mentally retarded girl with homocystinuria due to MTHFR deficiency among different therapeutic approaches only treatment with betaine gday resulted in a satisfactory BR betaine improved homocysteine remethylation and thus lowered plasma homocystine to trace amounts and normalized the previously very low plasma Met concentration this biochemical response was associated with a clinical improvement although she remained mentally retarded
|
MEDAL
|
the OD of acute pulmonary edema involves a complex interplay between the capillary hydrostatic interstitial hydrostatic and oncotic pressures and the capillary permeability we review the pathophysiological processes involved and illustrate the concepts in a number of common clinical situations including heart failure with normal and reduced ejection fractions mitral regurgitation and arrhythmias we also describe other rarer causes including exercise swimming and divinginduced acute pulmonary edema we suggest a unifying framework in which the critical abnormality is a mismatch or imbalance between the RA and left VVI stroke volumes in conclusion we hypothesize that increased RA ventricular contraction is an important contributor to the sudden increase in capillary hydrostatic pressure and therefore a central mechanism involved in the development of AM edema
|
MEDAL
|
rubredoxin dg rd is a small nonheme ironsulfur protein shown to function as a redox coupling protein from the sulfate reducing bacteria desulfovibrio gigas the protein is generally purified from anaerobic bacteria in which it is thought to be involved in electron transfer or exchange processes rd transfers an electron to oxygen to form water as part of a unique electron transfer chain composed by nadhrubredoxin oxidoreductase nro Rd and rubredoxinoxygen oxidoreductase roo in dg the crystal structure of dg rd has been determined by means of both a fe singlewavelength anomalous dispersion sad signal and the direct method and refined to an ultrahigh a resolution using xray from a synchrotron rd contains one iron atom bound in a tetrahedral coordination by the sulfur atoms of four cysteinyl residues hydrophobic and pipi interactions maintain the internal rd folding multiple conformations of the ironsulfur cluster and amino acid residues are observed and indicate its unique mechanism of electron transfer several hydrogen bonds including nhsg of the ironsulfur are revealed clearly in maps of electron density abundant waters bound to co MMP of residues val cys gly ala and gly which may be involved in electron transfer this ultrahighresolution structure allows us to study in great detail the relationship between structure and CF of rubredoxin such as salt bridges hydrogen bonds water structures cysteine ligands ISC and distributions of electron density among activity CS for the first time this information will provide a clear role for this protein in a strict anaerobic bacterium
|
MEDAL
|
the activity of the noradrenergic marker enzyme dopaminebetahydroxylase was measured in brains removed postmortem from CP and patients with alzheimers disease CEA was decreased in the frontal and temporal cortices and hippocampus in patients with alzheimers disease but was within the normal range in patients with depression multiinfarct dementia and terminal comathe decrease in enzyme activity in alzheimers disease may reflect an abnormality of cortical NE fibres in some patients with the disease
|
MEDAL
|
an evolutionarily conserved vertebrate homologue of the drosophila nk HD gene bagpipe nkx is expressed in V1 and visceral mesodermderived muscle tissues and may influence smooth muscle cell differentiation nkx was evaluated for mediating smooth muscle gammaactin smga gene activity a TPS marker of smooth muscle differentiation expression of mnkx in rat cv fibroblasts was unable to elicit smga promoter activity but required the coexpression of serum response factor srf to activate robust smga transcription a novel complex element containing a juxtaposed nkxbinding site nke and an srfbinding PE sre in the PT promoter region was found to be necessary for the nkxsrf coactivation of smga transcription furthermore nkx and srf associate through proteinprotein interactions and the HD region of nkx facilitated srf binding to the complex nkesre mutagenesis of nkx revealed an GABA domain within its CT segment in addition mnkxsrf cooperative activity required an IN nkx HD along with the mads box of srf which contains dna binding and dimerization structural domains and the contiguous CT srf activation domain thus smga is a novel target for nkx and the activity of nkx on the smga promoter is dependent upon srf
|
MEDAL
|
in the united states racial differences in the prevalence and incidence of hiv infection and aids diagnoses are dramatic these differences are C1 have been recognized for nearly years and are as yet not well investigated these disparities show no signs of diminishing and in fact are widening particularly among DUs and women most observers of the racial disparities in prevalence and incidence of hiv infections and aids diagnoses in the united states have concluded that these disparities exist because prevention messages supplies andor interventions do not effectively reach those at greatest risk of infection in essence such interpretations suggest that blacks and latinos continue to practice more risk behaviors than whites there are much data to suggest that this is in fact not true evidence from index injection drug users and of their drug and sexual network members participating in hiv prevention trials network is presented these data describe lower use andor access to drug treatment and needle exchange programs by black injectors in addition data indicate the coexistence of increased prevalence of hiv in the networks of uninfected black drug users and fewer associated risk behaviors in the networks of black and latino indices compared with networks of white indices understanding racial disparities in hiv is a critical challenge yet risk behaviors alone do not explain observed disparities in infection rates
|
MEDAL
|
the aim of this study was to investigate the relationship of interleukin il with vascular EC growth factor vegf and plasminogen activator system pa system in the progression of CC crc in eightyseven patients with crc the C2 of il and vegf as representative angiogenic factors and urokinasetype plasminogen activator upa upa receptor upar plasminogen activator inhibitor pai and pai as representative invasive AF were quantitatively assayed in tumor and adjacent normal tissues the C2 of il vegf and pa system AF in tumor tissues were all significantly higher than those in normal tissues the il level was significantly associated with tumor size depth of infiltration dukes stage and liver metastasis and also significantly correlated with the levels of vegf upar upa and pai the vegf level was significantly associated with tumor size vascular involvement the C2 of upar and pai were significantly associated with tumor size and depth of infiltration and the upar level was associated with CL metastasis the vegf level was significantly correlated with the levels of upar and pai these results reveal that il vegf and pa system AF are contributed to RT growth invasion and metastasis in crc univariate analysis revealed that high levels of il vegf and upar were significantly associated with a shorter overall survival time however MVA identified only CL metastasis as an independent prognostic factor in conclusion il is responsible to RT progression and liver metastasis of crc and the activation of pas induced by il as well as vegf may play an important role in the progression of crc
|
MEDAL
|
a longterm followup study of eyes that had had initially successful vitrectomies for complications of PDR showed that only ten eyes had significant worsening of VA at final examination to months T3 surgery compared to the best VA recorded during the first six months after surgery AS flat retinal neovascularization was present at final examination in only three eyes and in no case did elevated fibrovascular proliferation occur the type and incidence of important complications during the early and later postoperative periods indicated a low rate of late complications
|
MEDAL
|
fluordeoxyglucose fdg is the most common radiotracer used for petct studies it enters the cell because of the glucose transporter proteins gluts erythrocytic membrane skeletal muscle lymphocytes ovaries breast pancreas retina erythrocytes adipocytes ovaries testis skeletal muscle adipocytes ovaries myocardium breast small intestine testis kidney erythrocytes spleen leucocytes brain CL testis BB liver kidney liver pancreas heart muscle heart prostate BB we undertook this T0 to expand the knowledge about physiological uptake physiological uptake of fdg was in brain waldeyer ring adenoids palatine tonsils lingual tonsils SGs parotids submandibular tongue vocal cords cricoarythenoid muscle thyroid brown fat supraclavicular mediastinal neck pericardial fat around kidney around great vessels in the thorax subdiaphragmatic intercostals paravertebral myocardium breast thymus contractive muscles CL SP similar to the liver stomach intestine kidneys bladder uterus ovaries testes bone marrow esophagus and AS inflammatory PI
|
MEDAL
|
left azygos lobe is much rarer than right azygos lobe but cases have been reported computed tomography proved more reliable than plain radiography in the diagnosis of left azygos lobe in a yearold girl
|
MEDAL
|
a progressive decrease in podocyte number underlies the OD of GS and reduced kidney CF in aging nephropathy recent data suggest that under certain disease states parietal epithelial cells pecs begin to express proteins considered specific to podocytes to determine whether this phenomenon increases in aging kidneys and mo ad libitumfed and mo calorierestricted cr rats were studied single and double immunostaining were performed with antibodies to the pec protein paired box gene pax and tight junction protein claudin the podocytespecific protein wilms tumor wt and the proliferating cell protein ki imagej software measured bowmans basement membrane bbm length and glomerular tuft area in individual glomeruli from each animal to assess glomerular size the results showed that in aged ad libitum rats the decrease in number of podocytesglomerular tuft area was accompanied by an increase in the number of pecsbbm length at and mo p vs mo the increase in pec number was due to proliferation increase in paxki doublepositive cells aging was accompanied by a progressive increase in the number of glomerular cells double IF for pax and wt in contrast the control moold cr rats had no increase in glomerular size and podocyte and pec number were not altered these results suggest that although the number of pecs and pecs expressing podocyte proteins increase in aging nephropathy they are likely not sufficient to compensate for the decrease in podocyte number
|
MEDAL
|
we did a systematic search and synthesis of evidence on the incidence of invasive pneumococcal disease symptomatic disease and circulating streptococcus pneumoniae serotypes in western europe using data from studies published between and we calculated a weighted mean invasive pneumococcal disease and pneumococcal meningitis incidence rate per children aged years or younger within confidence intervals together with the prevalence of s pneumoniae serotypes and resistance to penicillin IPD incidence was cases per children under years ci QTc heptavalent conjugate vaccine serotypes account for of isolates among people aged under years of age of isolates in individuals aged under years were penicillin resistant the incidence of invasive pneumococcal disease appeared consistently lower in western EU countries compared with studies from the usa thus the use of studies of VE based on the us population may lead to an overestimation of the benefits of its introduction in europe
|
MEDAL
|
in tamoxifentreated breast CA patients the occurrence of hot flashes may be associated with ERP estrogen receptor antagonism dependent on genetic variations of metabolic enzymes and the estrogen receptor early breast cancer patients who were randomized to receive tamoxifen followed by exemestane within the tamoxifen exemestane adjuvant multinational trial were genotyped for five cypd alleles cypd genotypes and phenotypes were related to the occurrence of hot flashes as adverse event during the first year of tamoxifen use primary aim and the time to the occurrence of hot flashes as ae during the CR time on tamoxifen AA aim in addition exploratory analyses on genetic variants of other metabolic enzymes and two common polymorphisms in the estrogen receptor were performed no association was found between the cypd genotypephenotype or any other genetic variant and hot flashes during the first year only higher age was related to a lower incidence of hot flashes in the first year adjusted odds ratio ci p the esr pvuii xbai cg haplotype was associated with the time to the occurrence of hot flashes during the complete time on tamoxifen cgcg vs cgother otherother adjusted hazard ratio ci p in conclusion the cypd genotypes and phenotypes were not associated with the occurrence of hot flashes common polymorphisms in the estrogen receptor might predict hot flashes as common tamoxifen side effect although this finding needs replication
|
MEDAL
|
twentyone patients were admitted to the surgical unit for management of an i.t. mass following treatment of HD disease hd they consisted of males and females with a mean age of years range seven had recurrent hd had a nonhodgkin solid tumour hd recurrences were treated with repeated chemotherapy with prolonged survivals including relapse to months followup nonhodgkin tumours were lung carcinomas which were resected in cases with only prolonged survival months and malignant schwannoma hd recurrences are observed equally in both young males and females and are subsequent to any applications of recombinant adenovirus
|
MEDAL
|
we have previously shown that foreign antigens conjugated to alpha macroglobulin alpha m were effectively taken up by macrophages which was followed by a remarkable increase in the proliferation of immune TA we present in this report that the production of antibodies against viral proteins can also be effectively achieved when viral proteins were conjugated to alpha m and fed to the in vitro immune system proteins derived from kirstein mu sarcoma virus kimsv were partially purified by gel chromatography and conjugated to alpha m by the action of proteinases viral proteins conjugated to alpha m were taken up by thioglycolateinduced murine peritoneal exudate cells pec more effectively than the free viral proteins murine SP cells were then added to pecs fed with free viral proteins or with alpha m viral protein conjugates and the cell mixtures were incubated for five days each SC medium was then assayed for a TPS antibody production by ELISA CA elisa figures based on such assays revealed that the production of antibodies against viral proteins was ten times higher when the proteins were fed to macrophages in conjugated forms with alpha m
|
MEDAL
|
glycyrrhizae radix modulates the neurochemical and locomotor alterations induced by acute psychostimulants in rodents via gabab receptors this T0 investigated the influence of methanol extract from GR megr on repeated methamphetamine meth induced locomotor sensitization and CPP cpp a cohort of rats was treated with meth mgkgday for consecutive days subjected to days of withdrawal and then challenged with the same dose of meth to induce locomotor sensitization during the withdrawal period the rats were administered megr or mgkgday a separate cohort of rats was treated with either meth or saline every other day for days in methpaired or salinepaired chambers respectively to induce cpp these rats were also administered megr mgkg prior to every meth or cpp expression test pretreatment with megr and mgkgday attenuated the expression of methinduced locomotor sensitization dosedependently and mgkg megr significantly inhibited the development and expression of methinduced cpp furthermore administration of a selective gabab receptor antagonist sch prior to megr treatment effectively blocked the inhibitory effects of megr on locomotor sensitization but not cpp these results suggest that GR blocked repeated methinduced behavioral changes via gabab receptors
|
MEDAL
|
the fine structure of the keratinization of the papilla filiformis of the human tongue was described for the first time two biopsies of normal tongue tissue were fixed in phosphatebuffered glutaraldehyde postfixed in osmiumtetroxyde embedded in durcopan und contrasted ultrathin sections were examined by electron microscopes jem a and b the findings show a highly configurated epithelconnective tissue border with basal lamina and irregular hemidesmosomes the epithelial structure of the interpapillary area is MZ with the fine structure of the human buccal mucosa which was classified as nonkeratinized or incompletely keratinized accordingly a SG is missing after loss of nucleus and organelles surface cells become flattened parallel to the surface of the tongue and have a fine fibrillar cytoplasm the papilla filiformis is formed by cell growth along the AA connective tissue papillae and consequently tubelike epithelial structures appear in the papillary area basal and stratum spinosum cells show epidermal structural features a difference from epidermal and other oral epithelial cells becomes apparent for the first time in the stratum granulosum by the appearance of a great number of round small electrondense khg surrounded by ribosomes the khg are not associated with tonofilaments in the area of the walls of the tubes mum large electrondense structures are formed by F0 of several khg with further differentiation these C1 khg disintegrate into bulky or granular masses filling the cell cytoplasm and thus mask the tonofilaments the cells at the borders of the tubes show a great structural R2 T3 the disintegration of nucleus and organelles the cytoplasm is formed by randomly oriented filaments of different packing or by fiberbundles yet the interfibrilla embedding substance typical of orthokeratinization is mostly lackingmin some cells of the tubeborders masses of disintegrated khg substance are masking the fibrillar cell cytoplasm in other Az where the khg do not increase by fusion intense fibrillar packing and abrupt keratinization becomes apparent at the rim of the tube fully keratinized epithelial threads are regularly found these are lacking at the bottom of the tubes all surface areas show a strong tendency towards desquamation thus a more or less wide surface PI is formed on the tongue consisting of desquamated and disintegrated surface cells and of bacterial groups of little differentiated basal cells which can be considered as the initial material for an accelerated regeneration can be evaluated as a truly remarkable finding this accelerated regeneration might be due to an increased wear of the inclompletely keratinized cells of the bulk of the papilla filiformis of the human tongue based on the findings of this study an attempt was made to explain the pathological reactions of human tongue epithelium in various systemic diseases
|
MEDAL
|
use of a metabolizable tetrazolium salt was observed to facilitate assessments of RT cell drug sensitivity in the softagar colony formation assay enzymemediated staining permits discrimination between viable and nonviable CG of cells so that druginduced cytotoxicity is clearly identifiable by visual inspection as well as by computerized image analysis the technique appears to be especially useful in the evaluation of primary tumor cell cultures which often contain substantial numbers of nonviable cellular aggregates
|
MEDAL
|
the purpose of this T0 is to determine the life satisfaction GA health conditions and depression levels of mothers with disabled children and the problems they experienced this descriptive T0 was conducted between and december in a rehabilitation center that renders services for totally physically and mentallydisabled children in turkey mothers participated in the T0 the data were collected by using an information form the life satisfaction scale the general health questionnaire and the beck depression inventory general health questionnaire scores of the mothers who had another disabled child and BDI scores of the working mothers were significantly high in addition a significant difference was found between the scores of LSS general health questionnaire and beck depression inventory by mothers who had difficulties regarding care of their children were worried for their children and experienced uncertainty with regard to the future
|
MEDAL
|
each week i record audio summaries for every article in jacc as well as an issue summary while this process has been timeconsuming i have become very familiar with every paper that we publish thus i have personally selected the papers both original investigations and review articles from distinct specialties for your review in addition to my personal choices i have included manuscripts that have been the most accessed or downloaded on our websites as well as those selected by the jacc editorial board members there are approximately articles selected across this part series which represent less than of the papers submitted to the journal in in order to present the full breadth of this important research in a consumable fashion we will present these manuscripts over the course of issues in jacc part one includes the sections congenital HR disease coronary disease interventions cvd prevention health promotion CF cardiomyopathies genetics omics tissue regeneration and hypertension part two includes the sections imaging metabolic disorders lipids rhythm disorders statistics valvular heart disease and V1 medicine
|
MEDAL
|
in elderly highrisk surgical patients sutureless AV replacement avr should be an alternative to standard avr the potential advantages of sutureless aortic prostheses include reducing crossclamping and CP bypass cpb time and facilitating minimally invasive surgery and complex cardiac interventions while maintaining satisfactory hemodynamic outcomes and low rates of paravalvular leakage the current T0 reports our singlecenter experience regarding the early outcomes of sutureless AV implantation
|
MEDAL
|
since its introduction in percutaneous endoscopic gastrostomy peg has become the procedure of choice for longterm enteral feeding in patients who are unable to take adequate oral nutrition traditionally gastroenterologists have waited for hr after peg tube placement before initiating feedings however recently published data have described the safety and efficacy of initiating peg tube feedings as early as hr T3 peg tube placement the objectives of this T0 were to determine how many hours T3 peg tube placement practicing gastroenterologists initiate feedings and to determine whether they were aware of the pertinent literature a fourpage questionnaire was mailed to practicing gastroenterologists and the data gathered were analyzed using the sas statistical package a majority were aware of articles published in the last six years regarding the safety and tau of early initiation of FF after peg of interest however only were initiating feedings less than hr after peg tube placement in conclusion there exists a discrepancy between published recommendations and clinical practice regarding early initiation of feedings after peg tube placement in this group of gastroenterologists who were surveyed
|
MEDAL
|
it is hypothesized that the deliberate structural tailoring of compounds designed for drug use to increase the TPS plasma protein IB can be used to reduce FP hepatic metabolism to test the feasibility of this hypothesis a dataset of drugs with plasma protein IB of or above divided into three classes including acids bases and neutrals was analyzed among the drugs with ⥠plasma protein IB the fraction of the TD existing in free form in vivo free dose fraction decreased in the following order acids neutrals bases the order was different for the fraction of the TD that existed in plasma protein bound form plasma protein bound dose fraction acids neutmic analysis of biological samples similar approaches that would allow indepth analysis of the glycans carried by glycoproteins resolved by sdspage require special considerations in order to maximize recovery and sensitivity when using mass spectrometry ms as the detection method a L1 hurdle to be overcome in achieving highquality data is the removal of gelderived contaminants that interfere with ms analysis the sample workflow presented here is robust efficient and eliminates the need for inline hplc cleanup prior to ms gel pieces containing target proteins are washed in acetonitrile water and ethyl acetate to remove contaminants including polymeric acrylamide fragments olinked glycans are released from target proteins by ingel reductive βelimination and recovered through robust simple cleanup procedures an advantage of this workflow is that it improves sensitivity for detecting and characterizing sulfated glycans these procedures produce an efficient separation of sulfated permethylated glycans from nonsulfated sialylated and neutral permethylated glycans by a rapid phasepartition prior to ms analysis and thereby enhance glycomic and sulfoglycomic analyses of glycoproteins resolved by sdspage
|
MEDAL
|
pork temperatures of m longissimus were recorded from pigs transported in different ways T2 post mortem with the following results having been obtained following conventional transport pork values were significantly higher than they had been following container transport degrees c or degrees c pork values were clearly influenced by driving into the slaughterhouse high ambient temperatures and additional environmentborne effects prevalent on the day of slaughter genetic configurations transport and rest periods on the other hand proved of minor importance pork temperature was found to be unsuitable for any characterisation of pork quality since only unimportant correlations existed with juice retention and colour brightness and secured relationships were found to exist merely with the phvalue changes in rectal temperature and respiratory frequency following conventional transport were slightly stronger than those following container transport both of them were normalised after one or two hours and are well suitable for an assessment of preceding transport stress
|
MEDAL
|
sarcoidosis is a complex multiorgan granulomatous disease with a likely genetic component west african ancestry confers a higher risk for SA than european ancestry admixture mapping provides the most direct method to locate genes that underlie such ethnic R2 in disease risk we sought to identify genetic risk variants within four previouslyidentified ancestryassociated regionspp p pq and qqin a sample of african americans we used LR fit by generalized estimating equations and the mix score statistic to determine which variants within ancestryassociated regions were associated with risk and responsible for the admixture signal fine mapping was performed by imputation based on a previous genomewide association T0 significant SCV were validated by direct genotyping within the pp locus the most significant ancestryadjusted snp was rs p an intronic snp within the hladra gene that did not solely explain the admixture signal indicating the presence of more than a single risk variant within this wellestablished sarcoidosis risk region the locus on chromosome p revealed a novel sarcoidosis risk snp rs p within intron of the gene xlinked inhibitor of apoptosis associated factor xaf that accounted for the majority of the admixture linkage signal immunohistochemical expression studies demonstrated lack of expression of xaf and a corresponding high level of expression of its downstream target XIAP xiap in SA granulomas in conclusion ancestry and association fine mapping revealed a novel SA susceptibility gene xaf which has not been identified by previous genomewide association studies based on the known biology of the xiapxaf apoptosis pathway and the differential expression patterns of xaf and xiap in sarcoidosis granulomas we suggest that this pathway may play a role in the maintenance of sarcoidosis granulomas
|
MEDAL
|
foreign body aspiration is a leading cause of death in children aged less than year the removal of a foreign body poses a great challenge to the skill of the anaesthetist four cases are presented analysing the part played by modes of respiration in the dislodgement of a bronchial foreign body during its retrieval
|
MEDAL
|
despite increased concern for injury during surgical reconstruction of the sternoclavicular joint to our knowledge there are few studies detailing the vascular relationships adjacent to the joint
|
MEDAL
|
previous studies of the cortical control of human facial muscles documented the CSD of corticobulbar projections and the presence of intracortical inhibitory and facilitatory mechanisms yet surprisingly given the importance and precision in control of facial expression there have been no studies of the afferent modulation of corticobulbar excitability or of the plasticity of synaptic connections in the facial primary motor cortex face m in healthy volunteers we used standard single and PP transcranial magnetic stimulation tms methods to probe motorevoked potentials meps shortintracortical inhibition intracortical facilitation shortafferent and longafferent inhibition and PAS in relaxed and AS depressor anguli oris muscles singlepulse tms evoked BL meps at rest and during activity that were larger in contralateral muscles confirming that corticobulbar projection to lower facial muscles is bilateral and asymmetric with contralateral predominance both shortintracortical inhibition and intracortical facilitation were present bilaterally in resting and active conditions electrical stimulation of the FN paired with a tms pulse ms later showed no shortafferent inhibition but longafferent inhibition was present paired associative stimulation tested with an electrical stimulationtms interval of ms significantly facilitated meps for up to min the longterm potentiation evoked for the first time in face m demonstrates that excitability oto VAs and reduced septal longitudinal CS in obstructive hcmpatients with or without additional coronary i.a. disease cad andor hypertension ht
|
MEDAL
|
we determined the effect of iv administered beta adr agonist CB on pulmonary CF and on the response to histamine in healthy ponies measurements were made at base line and T3 saline solution or CB was po iv at a dosage of or microgramskg the dosage of clenbuterol used in each T0 was unknown to the investigators until all the data had been analyzed i.v. of saline solution or clenbuterol did not alter baseline pulmonary function significantly aerosol histamine administration significantly decreased PaO(2) and dynamic compliance and increased pulmonary resistance respiratory frequency and minute ventilation but had no effect on arterial carbon dioxide tension and tidal volume the magnitude of NC in these variables was unaffected by previous administration of clenbuterol at any of the dosages tested we conclude that clenbuterol at the dosages tested is not a bronchodilator in healthy ponies and does not exert a significant protective effect against aerosol histamineinduced airway narrowing
|
MEDAL
|
group a streptococci which produce streptolysin s sls contain a cellular potential hemolysin cph which is precursor to extracellular sls since the cellular location of cph is unknown protoplasts prepared with group c phageassociated lysin or mutanolysin from strains of group a streptococci were fractionated into subcellular components and assayed for cph in all strains most of the cph was membrane associated and most could not be removed from membranes by washing with buffer or m licl cph remaining in the cytoplasmic fraction was sedimentable but not associated with membrane fragments ribonuclease digestion neither solubilized nor inactivated cph from membranes streptococcal proteinase also did not affect cph although it did inactivate sls we conclude that group a streptococci contain a L1 pool of cph in the membrane and a smaller pool in the cytoplasm
|
MEDAL
|
different glucagonlike peptide glp molecular forms are produced in the pancreas and the small intestine by differential processing of proglucagon in this report molecular forms of glp in two human pancreatic endocrine tumors were studied and compared with those in the pancreas and small intestine a predominant glp immunoreactive form in the pancreas was eluted at the position of glp amide whereas a predominant immunoreactive form in the ileal mucosa wasrs responded to both PRs these results suggest that the ability of cat g to inhibit responses to thrombin but not sfllrn is due to cleavage of the receptor at phetrp deleting the tethered ligand domain and show that cat g can activate thrombin receptors but only if the cleavage site at phetrp is mutated or otherwise protectedabstract truncated at words
|
MEDAL
|
the form and function of the support skeleton musculature and teeth were examined in an ontogenetic series of pacific hagfish eptatretus stoutii spanning about a sixfold range in TL lt tooth area FF apparatus length basal plate size theoretical dental plate retractile force penetration force and applied tooth stress were measured relative to body size morphological variables eg tooth area and basal SP size scaled with positive allometry and PET variables eg retractile force and applied tooth stress scaled isometrically with lt these results suggest that juveniles do not undergo ontogenetic dietary changes and consume functionally equivalent prey to adults although adults can grasp proportionally lous aortic tissue in the surgical treatment of left main coronary ostioplasty and reported the short and longterm outcomes of the patients
|
MEDAL
|
Subsets and Splits
No community queries yet
The top public SQL queries from the community will appear here once available.