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patients with persistent somatoform pain disorder pspd usually experience various functional impairments in pain emotion and cognition which cannot be fully explained by a physiological process or a PCS disorder however it is still not clear for the mechanism underlying the pathogenesis of pspd the present T0 aimed to explore the intra and internetwork functional connectivity fc differences between pspd patients and healthy controls hcs functional magnetic resonance imaging fmri was performed in pspd patients and age and gendermatched hcs we used independent component analysis on restingstate fmri data to calculate intra and internetwork fcs and we used the twosample ttest to detect the fc differences between groups spearman rho analysis was employed to evaluate the correlations between fcs and clinical assessments as compared to hcs pspd patients showed decreased coactivations in the right superior temporal gyrus within the anterior defaultmode network and the ACC within the salience network and increased coactivations in the bilateral supplementary motor areas within the sensorimotor network and both the left posterior cingulate cortex and the medial prefrontal SC within the anterior defaultmode network in addition we found that the pspd patients showed decreased fncs between sensorimotor network and audio network as well as visual network between defaultmode network and executive control network as well as audio network and between salience network and executive control network as well as RA frontoparietal network and increased fncs between sensorimotor network and left frontoparietal network salience network asd on dynamic warping dw is proposed for patients with intellectual disability it uses an eeg template of an individual subjects dominant seizure type to extract the morphological features from eeg signals a LDA lda classifier is used to perform the seizure detection results show that the dwbased feature in the frequency domain is superior than that in the time domain and the features extracted using WT method
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the incidence of thromboembolic complications was compared retrospectively in CAD kidney allograft recipients treated with cyclosporin and lowdose steroids and the same number of cadaveric kidney allograft recipients treated with azathioprine antilymphocyte AHG and highdose CS in the cyclosporin group TE complications occurred in patients PE renal vein thrombosis deep vein thromboses and haemorrhoidal thromboses in the azathioprine group the only TE complication was episode of SF thrombophlebitis haemostatic tests in cyclosporintreated and azathioprinetreated patients and normal subjects in each group showed increased concentrations of factor viii c fibrinogen antithrombin iii and protein c in the cyclosporintreated patients adenosinediphosphateinduced platelet aggregation was also significantly enhanced in the cyclosporin group the effect of cyclosporin on haemostasis may predispose to thromboembolic complications
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electromagnon excitations in multiferroic orthorhombic rmno are shown to result from the heisenberg coupling between spins despite the fact that the static polarization arises from the much weaker dzyaloshinskiimoriya exchange interaction we present a MM incorporating the structural characteristics of this family of manganites that is confirmed by far infrared transmission data as a CF of temperature and magnetic field and inelastic neutron scattering results a deep connection is found between the magnetoelectric dynamics of the spiral phase and the static magnetoelectric coupling in the collinear e phase of this family of manganites
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reduced fullerenes and fullerene derivatives exhibit intense absorptions in the visnearir visnir region the absorptions are CS toward the addition pattern number of addends and oxidation state of the fullerene species and are used as an important benchmark for identifying anionic fullerene species similar absorptions are also shown for the reduced singly bonded c species which are electronically different from reduced fullerene derivatives however much less work has been carried out on the visnir spectroscopic study of the anionic singly bonded c species likely due to the difficulty in controlled production of these species herein we report the visnir spectroscopic T0 of the mono and dianions of the singly bonded c species rc rc and rc⢠which are selectively generated by controlledpotential bulk electrolysis cpe of the singly bonded c dimer and c oxazolino heterocycles timedependent density functional theory tddft calculations were performed to rationalize the exp results
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this article reviews the evidence for computerbased AEP training cbat in children with langons to determine the differences in the predatory ability among beetle species inhabiting rice fields the following species were introduced to cx tritaeniorhynchus th instars in a plastic cup smallbodied species mm in body length comprising hydroglyphus japonicus noterus japonicus laccophilus difficilis hyphydrus japonicus and agabusjaponicus mediumbodied species mm in BL comprising hydaticus rhantoides hydaticus grammicus rhantus suturalis eretes griseus hydaticus bowringii agabus conspicuous and graphoderus adamsii and largebodied species mm comprising cybister brevis and c japonicus the average h predation rate was highest in mediumbodied species followed by smallbodied species and largebodied species the PET responses to cx tritaeniorhynchus larvae of mediumbodied species h grammicus r suturalis and e griseus were estimated eretes griseus exhibited the highest attach rate and shortest preyhandling time suggesting that mediumbodied diving beetles especially e griseus may be efficient predators of mosquito larvae in rice fields
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CBF infarction was documented by arteriography and serial computed cranial tomography ct in four young adults ages to years with migraine in one case posterior cerebral i.a. occlusion produced a deep parietotemporal infarct the other three cases all had frontotemporal infarcts one hemorrhagic in the territory of the MCA i.a. without major arterial occlusion two infarcts produced lasting neurologic deficits one was associated with mild transitory PS and one was asymtomatic laboratory investigations in two cases revealed no hematologic or cardiovascular predisposition to cerebrovascular disease cerebral infarction as revealed by ct may be more prevalent in complicated migraine than is generally appreciated such lesions may or may not develop in chronologic and anatomic relationship to the headache and may involve either large or small arteries the prognosis for PET REC based on this limited sample seems favorable
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limitcycle oscillations induced by time delay are widely observed in various systems but a systematic phasereduction theory for them has yet to be developed here we present a practical theoretical FR to calculate the phase response CF zθ a fundamental quantity for the theory of delayinduced limit cycles with infinitedimensional phase space we show that zθ can be obtained as a zero eigenfunction of the adjoint equation associated with an appropriate bilinear form for the delay differential equations we confirm the validity of the proposed framework for two biological oscillators and demonstrate that the derived phase equation predicts intriguing multimodal locking SMB
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laboratory investigation of hemoglobinopathies includes CBC cbc hemoglobin hb typing by high performance liquid chromatography hplc and dna analysis dna analysis is the most reliable method but requires a manually laborious procedure and is time consuming a more practical method of detecting abnormal hbs is the hplc technique because it is more rapid and easier to interpret hb constant spring hb cs hba ct c is an abnormal variant that is labile and difficult to detect using conventional methods to evaluate the efficiency of hb cs determination by hplc blood samples from subjects were analyzed using an automated cell analyzer for hematological parameters automated hplc for hb identification and PCR reaction pcr for αthalassemia αthal and hb cs confirmation these included normal heterozygous αthal homozygous αthal heterozygous αthal heterozygous hb cs seven homozygous hb cs and compound heterozygous αthal and hb cs subjects the results showed that sensitivity specificity positive predictive value ppv and negative predictive value npv of hb cs by hplc were and respectively the mean of misdiagnosis value of the three groups of hb cs subjects total was n with percentages for heterozygous hb cs homozygous hb cs and compound heterozygous αthal and hb cs being and respectively the hplc method yielded good results although it may also lead to misdiagnosis of hb cs due to the relatively small amount and lability
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in some rat CS combinations preoperative donorspecific ABT produces LT renal allograft survival although the underlying mechanisms are unclear this study has examined whether fc receptor fcrblocking activity could be detected in the serum of unmocitol deoxyglucose and carbohydrate metabolism was studied the concentration of anhydrodglucitol was estimated by means of gasliquid chromatography in normally nonpregnant women the concentration was found to be mgl mean sd during the NP from weeks of gestation a steadily decreasing concentration was observed as the pregnancy progressed and the lowest value mgl was found in the third trimester after days of puerperium the concentrations were found to be mgl on the th day postpartum the level was within the range for nonpregnant subjects the values in pregnant women with diabetes mellitus and gestational diabetes mellitus were mostly below mgl throughout the entire pregnant period the 1,5-AG concentration was not affected by meals or oral gl loading a concentration below mgl was found in of the normally Pr women where oral glucose tolerance tests and measurement of glycohemoglobin were shown to be within the normal range the present T0 suggests that a NC of 1,5-AG level during pregnancy may reflect a mild alteration of carbohydrate metabolism that goes undetected by all the other diabetic indicators
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analysis of the bg antigens of eight chicken L1 H-2 complex b system recombinant haplotypes by high resolution 2DE gel electrophoresis has provided evidence for the transfer of the CR bg subregion in seven cases in the eighth a partial duplication within the bg subregion appears to have occurred in this recombinant the entire array of polypeptides associated with one parental allele bg is expressed together with nearly the entire array of bg polypeptides of the other parental haplotype b this compound polypeptide DP corroborates the serological evidence for a partial duplication within the bg subregion and provides indirect evidence for the existence of multiple loci within bg and for a means by which polymorphism may be introduced into the chicken L1 histocompatibility complex
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the present T0 examined the effects of muscle activity modulation on leg stiffness during an exhaustive SSC ssc exercise in eight male subjects reaction force electromyography emg of the soleus sol gastrocnemius ga and vastus lateralis vl muscles and sledge seat position were recorded during the ssc exercise consisting of maximal intermittent drop jumps followed by a continuous submaximal jumping until exhaustion on a sledge apparatus metabolic loading was determined by measuring blood lactate la no change was found in leg stiffness during the maximal jumps whereas the subsequent submaximal jumping induced a significant reduction by p leg stiffness was closely related to the emg ratio between the braking and pushoff phases in sol r p and particularly in ga r p but not in vl r ns at the end of the submaximal jumping furthermore the postexercise la was significantly associated with the emg ratio at the end of the submaximal jumping in sol r p and ga r p these results indicate that activity modulation between the braking and pushoff phases in the triceps surae muscle particularly in ga plays an important role in leg stiffness adjustments during fatiguing ssc exercise it is suggested that efficient activity modulation ie high emg ratio of t spinal surgery careful daily substitution of up to l of hypotonic fluids led to full recovery within days NDI should always be considered in lithiumtreated patients undergoing elective surgery in order to avoid severe hypernatraemia
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our previous work indicated a role for fynkinase in mediating several ethanol and gabaa agonistmediated behaviors in the present work we investigate behavioral sensitivity to ethanol and several gabaa EDC in mice that overexpress fynkinase in forebrain to further characterize the role of this nonreceptor tyrosine kinase in the mediation of ethanol sensitivity transgenic mice overexpressing fynkinase were tested for sensitivity to ethanolinduced loss of righting reflex and ethanol preference drinking using a twobottle choice drinking paradigm LRR induced by tetrahydroisoxazolocpyridinol thip gabaa agonist and etomidate gabaa positive allosteric modulator were also assessed fyn overexpressing mice exhibited shorter durations of ethanolinduced loss of righting reflex in the absence of differences in the rate of blood ethanol clearance and exhibited reduced ethanol preference drinking the genotypes did not differ in initial sensitivity to ethanolinduced LRR suggesting OD of greater acute tolerance to this ethanol action fyn overexpressing and WT also did not differ in sensitivity to LRR induced by thip and etomidate the present results suggest regional specificity for fynkinase in the modulation of ethanol and gabaergic behavioral sensitivity fynkinase overexpression in forebrain structures modulates ethanols hat was examined endurancetrained males n performed constantload exercise tests to exhaustion at vomax in the heat AT c before and after d of cr g x d cr g x d glucose polymer or PL cr increased intracellular water and reduced thermoregulatory and cardiovascular responses eg HR rate rectal temperature sweat rate but did not significantly increase time to exhaustion min vs min p time to exhaustion was increased significantly in subjects whose estimated IM cr C2 were substantially increased NR min vs min p crinduced hyperhydration can result in a more efficient thermoregulatory response during prolonged exercise in the heat
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BV phospholipase a bvpla is a hydrolytic enzyme that specifically cleaves the sn acyl bond of phospholipids at the lipidwater interface the same enzyme is also believed to be responsible for some SVR anaphylactic reactions in bee venom sensitized individuals to T0 the structurefunction relationships of this enzyme and to define the molecular determinants responsible for its allergenic potential a synthetic gene encoding the mature form of bvpla was expressed in escherichia coli this enzyme was produced as a fusion protein with a xhistag on its aminoterminus yielding mg of fusion protein per of culture T3 metal ion affinity chromatography a kallikrein protease recognition site was engineered between the xhistag and the aminoterminus of the enzyme allowing isolation of the protein with its correct nterminus recombinant affinity purified bvpla was refolded purified to homogeneity and cleaved with kallikrein resulting in a final yield of mg of AS enzyme per of SC the enzymatic and immunological properties of the recombinant bvpla are MZ to enzyme isolated from bee venom indicating a nativelike folding of the protein
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despite progress in ART the lack of control of implantation remains a major obstacle to obtain successful pregnancies it is of prime importance to determine the characteristic features of a receptive endometrium embryo implantation is a complex event involving apposition followed by the adhesion of the blastocyst to the maternal endometrium and finally the invasion of this endometrium though implantation could occur in any human tissue the endometrium is the only tissue where embryo implantation cannot occur except during a restricted period called the implantation window during this window the endometrium is highly receptive to the embryo maternalembryo crosstalk is favored by the implantation window the molecular basis of the implantation window remains to be defined in the present T0 we investigated the role of hcg tgfbeta igf and igf in the secretion of LIF lif and interleukin il by human epithelial endometrial cells in culture epithelial endometrial cells were obtained from endometrial biopsy in fertile women hcg and tgfbeta added to primary cultures of human epithelial endometrial cells showed a stimulatory effect on lif secretion and a reducing effect on il secretion all these findings suggest a role for hcg and tgfbeta in human embryo implantation
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nodular skin lesions on the lateral aspects of the legs of a female child were first noticed at the age of days a biopsy of the lesions was done at the age of months when the child had also developed C2 and inguinal lymphadenopathy and angiomatous lesions on the face the diagnosis of RDD rdd also known as sinus histiocytosis with massive lymphadenopathy shml was made increasing respiratory obstruction by lymphoid tissue prompted a week trial with oral prednisolone a dramatic response occurred with CR resolution of all clinical findings within days but with recurrence of lymphadenopathy weeks after stopping with medication further observations over the next years established a consistent response to prednisolone and a DP of steroid dependence
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a yearold woman presented with recurrent urinary tract infections a computed tomography of the abdomen and pelvis shows a dilated and malrotated right renal pelvis consistent with a ureteropelvic junction one as well as multiple renal arteries arising from the aorta a computed tomography angiography revealed separate renal arteries originating from the aorta with a single renal i.a. crossing over and obstructing the right renal pelvis on lasix renogram the affected kidney contributes of total renal function the patient remained asymptomatic absent of hematuria flank pain infection and opted for AS surveillance
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a yearold girl with poor WG and pubertal delay was referred for hyperthyroidism slightly elevated C2 of circulating thyroid hormones failed to suppress circulating thyrotropin tsh C2 despite AGA thyrotropinreleasing hormone stimulation of tsh secretion a thyrotropinsecreting pituitary adenoma was identified as the cause of inappropriate tsh secretion
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guanylate cyclase of every fraction studied showed an absolute requirement for mn ions for optimal activity with mg or ca reaction was barely detectable triton x stimulated the particulate enzyme much more than the supernatant enzyme and solubilized the particulateenzyme activity substantial amounts of guanylate cyclase were recovered with the washed particulate fractions of cardiac muscle skeletal muscle CBF SC and liver of various species the S9 of these tissues contained of total MICs in frog heart the bulk of guanylate cyclase was present in the supernatant fluid plasmamembrane fractions contained and respectively of the total homogenate guanylate cyclase MICs present in skeletal muscle rb cardiac muscle guinea pig CL rat and cerebral SC rat in each case the TPS activity of this enzyme in PMs showed a five to tenfold enrichment when compared with TH TPS activity these results suggest that guanylate cyclase like adenylate cyclase and ouabainsensitive na kdependent atpase adenosine triphosphatase is associated with the surface membranes of cardiac muscle skeletal muscle CL and CBF cortex however considerable MICs are also present in the supernatant fractions of these tissues which contain very little adenylate cyclase or OS na kdependent atpase MICs
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this article describes contemporary controversies regarding two categories of softtissue pain stpchronic widespread pain and fibromyalgia syndrome the tone is more editorial than review didactic it draws upon history to explain current trends that project possible future implications it begins with an orientation to classification of stp pain conditions and contrasts two ways to make the fibromyalgia diagnosis epidemiological data will be placed in perspective the article ends with the voice of a nonphysician patient advocate stp classification divides relevant painful conditions into three subgroups depending on the extent of body involvement localised regional and generalised fibromyalgia syndrome in the generalised stp category is distinguished from other types of chronic widespread pain by virtue of its greater severity during the past years the diagnosis of fibromyalgia was based on a research classification american college of rheumatology research classification criteria acr rcc that requires a PH of chronic widespread pain and the examination finding of widespread mechanical allodynia a new RPA american college of rheumatology fibromyalgia diagnostic criteria acr fdc validated for clinical use still requires a history of CWP but the examination is replaced by a historical assessment of comorbid symptom severity the populations identified by the two criteria are similar but not MZ misuse of the new criteria could expand fibromyalgia from to of the GP avoidance of the term fibromyalgia could return it to the obscurity from whence it came leaving a much larger problem in its stead
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previous T0 has shown that photoperiod and age affect tissue accumulation of Cd cd in a small rodent the bank vole since the body mass is also influenced by these factors the present T0 was designed to determine whether massspecific daily metabolic rate might be responsible for differential accumulation of cd in the liver and kidneys of the short and longphotoperiod bank voles as well as of the young and old animals one and fivemonth old male bank voles were held under short h CS h dark or long h CS h dark photoperiods and exposed to dietary cd microgg for weeks the bank voles raised under the short LD and those injected subcutaneously with melatonin micromolkgday under the long LD showed significantly higher concentrations of cd in the liver and kidneys than the agematched longphotoperiod animals the old bank voles accumulated significantly less cd in both organs than the young animals these differences in cd accumulation appeared not to be associated with the relative cd intake however the hepatic and renal cd levels followed a DP similar to that of the massspecific daily VO2 or energy expenditure and energy AE these data indicate that massspecific daily metabolic rate and SE AE an indicative of digestive and absorptive processes may be responsible for differential tissue cd accumulation in the bank vole
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homologies among bacterial signal transduction proteins suggest that a common mechanism mediates processes such as CTX osmoregulation sporulation virulence and responses to nitrogen phosphorous and oxygen ED a common kinasemediated phosphotransfer reaction has recently been identified in chemotaxis nitrogen regulation and osmoregulation in chemotaxis the chea kinase passes a phosphoryl group to the cytoplasmic protein chey which functions as a phosphorylationactivated switch that interacts with flagellar components to regulate motility we report here the xray crystal structure of the salmonella typhimurium chey protein the determination of the structure was facilitated by the use of sitespecific mutagenesis to engineer heavyatom binding sites chey is a singledomain protein composed of a doubly wound fivestranded parallel betasheet the phosphoacceptor site in chey is probably a cluster of asparticacid side chains near the CT edge of the betasheet the pattern of CS similarity of chey with components of other regulatory systems can be interpreted in the light of the chey structure and supports the view that this family of proteins have a common structural motif and active site
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the carcinogen nitroquinolineoxide was applied to the palatal mucosa of rats for varying periods of time this tissue was separated into epithelial and connective components and then recombined in various combinations and implanted into hypothymic mice seventytwo per cent of the implants were recovered and were suitable for detailed histological analysis the technique provides a suitable MM for the assessment of epithelialconnective tissue interactions in experimental oral mucosal carcinogenesis
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heparanase is a HS Endo which was previously detected in the outer root sheath of murine hair follicles HPA overexpression was reported to improve mouse hair regrowth in this T0 we investigated its involvement in human hair biology immunofluorescence PCD was used to explore heparanase CSD in both anagen and catagen hair follicles HPA functionality was assessed in in vitro cultured hair follicles in the presence of a heparanase activity inhibitor our results showed that heparanase expression was i primarily located in the inner root sheath irs of human hair F2 and there ii restricted to anagen phase furthermore inhibition of heparanase in in vitro cultured hair follicles induced a catagenlike process hair shaft retreat upward was accompanied by a decrease in kipositive cells the formation of an epithelial strand as evidenced by k keratin expression and the loss of irs as assessed by transglutaminase and desmoglein labelling irs CSD of heparanase and the induction of catagenlike involution of hair follicles when a potent heparanase inhibitor is added suggest that HPA is a key actor of irs differentiation and hair homeostasis
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neonatal polymorphonuclear WBCs pmns have previously been shown to be chemotactically deficient to probe the mechanisms responsible for this deficiency we have investigated the phenomenon of concanavalin ainduced capping in neonatal pmns pmns from cord blood of healthy FT infants and normal AD volunteers were isolated by standard ficollhypaque and dextran sedimentation after incubation with and without colchicine the cells were reincubated with fluorescein isothiocyanatecon a fixed and prepared in wet mounts using a fluorescence microscope pmns were identified and percentage of the capped cells was counted upon treatment with colchicine adult pmns showed a significant increase in the percentage of capped cells by contrast the CB pmns showed no significant increase in capping T3 colchicine treatment the difference between percentage of pmns showing colchicineinduced capping in AD and cord blood was highly significant p less than students t test
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a quantitative comparison has been carried out between laser scanning confocal microscopy on living cells and standard electron TSM methods on fixed samples it was estimated from these measurements that there was about reduction in thickness in fixed samples of monolayer v hamster cells precise information on the true thickness of living cells as irradiated is required for full interpretation of radiobiological data with poorly penetrating radiations including ultrasoft xrays the confocal TSM allows rapid measurements on unperturbed living samples
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many different phylogenetic clustering techniques are used currently one approach is to first determine the topology with a common clustering method and then calculate the branch lengths of the tree if the resulting tree is not optimal exchanging tree branches can make some local changes in the tree topology the whole process can be iterated until a satisfactory result has been obtained the efficiency of this method fully depends on the initially generated tree although local changes are made the optimal tree will never be found if the initial tree is poorly chosen in this article genetic algorithms are applied such that the optimal tree can be found even with a bad initial tree topology this tree generating method is tested by comparing its results with the results of the fitch program in the phylip software package two simulated data sets and a real data set are used
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a yearold man sustained a severe HI and had a torn posterior meningeal artery that caused massive intraventricular hemorrhage traumatic PA of the posterior meningeal artery should be considered in cases where intraventricular hemorrhage occurs in the presence of occipital bone fracture and contiguous epidural hematoma vertebral angiography is of value in this regard
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gastric carcinoma gc is one of the most common causes of cancerrelated death worldwide surgical resection is the only cure available and is dependent on the gc stage at presentation which incorporates pT extent of lymph node and distant metastases accurate preoperative staging is therefore essential for optimal surgical management with consideration of preoperative andor postoperative chemotherapy multidetector computed tomography mdct with its ability to assess RT depth nodal disease and metastases is the preferred technique for staging gc EUS is more accurate for assessing the depth of wall invasion in early cancer but is limited in the assessment of advanced local or stenotic CA and PCD of distant metastases magnetic resonance imaging mri although useful for staging is not proven to be ERP positron emission tomography pet is most useful for detecting and characterizing distant metastases both mdct and pet are useful for assessment of treatment response following preoperative chemotherapy and for PCD of recurrence T3 surgical resection this review article discusses the usefulness of imaging modalities for detecting staging and assessing treatment response for gc and the potential role of newer applications including ct volumetry virtual gastroscopy and perfusion ct in the management of gc
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programmed electrical stimulation in anesthetized marmoset monkeys was used to examine relative antiarrhythmic efficacies of dietary n and n polyunsaturated fatty acids pufas from fish and plant oils diets contained of SE en as fat comprising en saturated fat and en pufas obtained by blending sheep fat with sunflower seed sfsso or fish oil sffo and a base diet T3 wk FF VVI fibrillation vf was inducible in of animals on each diet under control conditions the vf threshold vft was significantly elevated in the sffo group ma n compared with the sfsso group ma n vft reduced during acute myocardial ischemia with of animals inducible per diet remained significantly higher with sffo feeding the sffo diet contained en as n pufas which was incorporated as of myocardial membrane fatty acids dietary n pufa reduced vulnerability of normal or ischemic myocardium to arrhythmias in a nonhuman primate
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timelimited psychodynamic therapies in the outpatient setting are offered for a broad spectrum of psychogenic disorders metaanalyses prove their effectiveness mainly for neurotic disorders we are interested in the suitability of the here applied cmpsasb MM tress et al of psychodynamic shortterm therapy for patients with psychosomatic and severe personality disorders we extensively present the concept of cyclic maladaptive DP cmp in its clinically relevant parts as the core of psychogenic pathological developments particularly the introject as the patients attitude towards him or herself is of great importance for therapeutic change and its followup development within the dkzp duesseldorf shortterm psychotherapy project male and female therapists mostly interns at the local university clinic treated patients m f with personality disorders n and psychosomatic disorders n as research groups and in comparison neurotic patients n in a naturalistic design treatments have been completed so far patients have dropped out the remaining are still in therapy relevant research instruments are the beeinträchtigungsschwere score bss an impairment score global assessment of functioning scale gaf cyclic maladaptive DP cmp symptom check list sclr and structural analysis of social behavior sasb including process and content ratings as well as the intrex questionnaire the treatments last sessions we carried out followup examinations after months year and years effect sizes for the whole sample by bss and gaf are remarkably high with further increase at followup interviews psychosomatic patients came off best but personality disorders in comparison to neurotic patients profited surprisingly well ESs in selfrating scl and intrex were less high however in selfrating the changes of social functions are not sufficiently addressed as a result our form of focal therapy is well suited not only for neuroses but also for psychosomatic disorders and PDs an enlargement of the indication spectrum for this form of psychodynamic therapy to include these disorders is well founded and promising
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a yearold man presented with fever of unknown origin generalized weakness edema and renal failure he had leftsided pleural effusion generalized lymphadenopathy multiple nontender cutaneous nodules hepatomegaly renal failure and hypergammaglobulinemia axillary lymph node biopsy showed findings consistent with castleman disease of the hyaline V1 type associated with interfollicular plasmacytosis a renal biopsy performed in view of proteinuria and acute renal failure showed hypercellular glomeruli with capillary loop thickening and double contours consistent with membranoproliferative glomerulonephritis skin nodule biopsy showed a glomeruloid hemangioma characterized by dermal proliferation of capillary loops in a nodular manner resembling a glomerulus he experienced clinical and biochemical remission with CS discontinuation of steroid therapy was associated with recurrence of renal failure reappearance of nodules lymphadenopathy and appearance of bony lytic lesions biopsy of bony lytic lesions showed plasmacytoma the patient achieved pCR on treatment with steroids and cyclophosphamide and is free of symptoms at the end of months of followup to our knowledge this is the first case report of the occurrence of membranoproliferative glomerulonephritis glomeruloid hemangioma of the skin and plasmacytoma in a patient with multicentric castleman disease without HIV
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cyclindependent kinase cdk participates in opioid receptor signalling through complex molecular mechanisms the acute effects of selective μfentanyl and δsnc opioid receptor agonists as well as the chronic effects of morphine the prototypic opiate agonist mainly acting at μreceptors modulating cdk and activators pp and their interactions with neurotoxicapoptotic factors dopamine and campregulated phosphoprotein of kda darpp and extracellular signalregulated kinase erk were quantified western blot analyses in the rat corpus striatum andor CBF SC to assess the involved mechanisms mdl was used to inhibit calpain activity and sl to disrupt mek erk kinaseerk activation acute FE mgkg and snc mgkg induced rapid min to fold increases of p content without induction of cdkp proapoptotic cjun nhterminal protein kinase or aberrant cleavage of polyadpribosepolymerase a hallmark of apoptosis in contrast fentanyl and snc stimulated cdkmediated pthr darpp pka inhibition and pthr mek mek inactivation and this latter effect resulted in uncoupling of mek to erk signals calpain inhibition with mdl cleavage of p to p attenuated fentanylinduced p accumulation but not the stimulation of pthr mek or pthr darpp mekerk inhibition with sl fully prevented fentanylinduced p upregulation notably chronic morphine treatment mgkg for days also increased p content and pp ratio and activatedinactivated mek in rat BB SC which indicated that p upregulation persisted under the sustained stimulation of μopioid receptors the results demonstrate that the acute stimulation of or leads to upregulation of p activator through a mekerk and calpaindependent pathway and to disruption of mekerk signalling by a cdkpinduced mek inhibition moreover the effects induced by the sustained stimulation of μreceptors with morphine suggest the participation of cdkp complex in opiateinduced LT neuroplasticity
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in january a yearold woman who had undergone RT resection for pleomorphic adenoma of the left PG four times since her first T0 in visited an orthopedist in our hospital complaining of pain in her right lower limb computed tomography ct of the whole body showed multiple PT on the left parotid gland right kidney the sacrum and both lungs biopsy of the S3 region and right nephrectomy were performed based on a clinical diagnosis of sacral and pulmonary metastases from renal cell carcinoma and palliative radiation therapy was immediately begun on the sacral region given the definitive pathological diagnosis of metastasis of pleomorphic adenoma of the parotid gland the recurrent RT of the left PG and the surrounding lymph NO were first removed postoperative findings demonstrated that the pleomorphic adenoma had metastasized to the CL NO the lung PT were resected subsequently and postoperative findings led to the diagnosis of pleomorphic adenoma metastasis of pleomorphic adenoma is known to be extremely rare and to our knowledge this case of metastasis to a kidney is the first reported in japan
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we demonstrate a convenient singlestep quantitation technique for dsDNA dna dsdna fragments in PCR reaction pcr products based on microchip capillary PACE microceuv or fluorescence detection pcr products of polymorphisms on the human ychromosome related to spermatogenic failure did not need purification they were premixed and comigrated with a dna digest whose concentration was known hydroxyethyl cellulose hec dissolved in x TBE x tbe ph was used as a separation matrix in a linear polyacrylamidecoated quartz microchip while mixed polyethyl oxides peos of different molarmasses dissolved in x tbe ph containing ngmicrol ethidium bromide was used as a separation matrix in an uncoated polymethyl methacrylate pmma microchip elution profiles were monitored under either realtime linear imaging uv detection in the snapshot mode where the total separation time is fixed or lightemitting diode led confocal fluorescence detection in the finishline mode where solutes migrate over the same separation length it is found that in both modes a linear relation exists between the peak areas a and the multiplication of the digest concentrations c and the fragment sizes l in a dna restrictive digest using the comigration electropherogram of a singlestep experiment the concentrations of pcr products were directly determined using the a versus lc linear relationship the sole condition to obey is that the chosen digest has different fragment sizes with the pcr products of interest this condition is easy to obey because microce owns high separation ability and many digests are commercially available the REC of the technique was between and the rsd for chiptochip concentration measurements was less than n hence the technique was accurate and reliable for dna assays
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spodoptera litura f is an important pest of numerous agroeconomic crops including green manure cover crops in taiwan sesbania sesbanin roxburghii merr sunn hemp crotalaria juncea l and rapeseed brassicae campestris l variety chinensis are the most popular green manure crops sesbania and sunn hemp are commonly planted in warm seasons whereas rapeseed is grown in the winter in this T0 lifetable data for s litura reared on these three green manures were collected to evaluate their roles as refuges of this pest the net reproductive rate intrinsic rate of increase and finite rate of increase of s litura were the highest when reared on sesbania F1 d d followed by sunn hemp offspring d d and rapeseed offspring d d the high growth rates on these green manure crops show that they can serve as potential breeding sites for s litura population projection demonstrated the rapid growth of s litura on sesbania sunn hemp and rapeseed as well because most growers have traditionally ignored pest management in green manure fields the mass emergence of s litura in these fields may cause unexpected infestations in nearby vegetable corn and peanut crops this study shows that the use of green manures as sources of nutrients should be critically reassessed and an areawide pest management program should be instituted by taking the population of s litura in green manure fields into consideration
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the possibility to differentiate normal squamous epithelium from dysplasticcis in the human esophagus by means of quantitative morphometry was investigated in surgical specimens from consecutive patients the epithelium was divided into three equal zones and a total of areas was investigated of all variables employed the interzonal R2 between the three epithelial layers was the one showing the highest degree of efficiency in discriminating normal esophageal epithelium from dysplasticcis lesions nuclear density nuclear area nuclear irregularity nuclear parallelity and nuclear ovality were the most efficient discriminatory individual morphological variables a sensitivity in discriminating normal squamous epithelium from dysplasticcis through morphometric evaluation was achieved by considering nuclear area in the superficial zone nuclear perpendiculairty in the intermediate zone nuclear density in the total epithelial thickness and interzonal variation of these variables on the other hand the specificity in discriminating dysplasiacis lesions from normal squamous epithelium was only this was due to the fact that one case by visual impression considered as dysplasia was morphometrically recorded as normal the view of that case showed that our initial visual impression of a dysplastic lesion proved to be erroneous and today that case would be recorded as a reactive lesion due to chronic inflammation
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blood from cattle with subclinical mycobacterium avium subsp paratuberculosis infection was stimulated with m avium subsp paratuberculosis antigens and expression of interleukinbeta ilbeta tumor rTNF TNF rantes mo chemoattractant protein mcp and il was measured expression of tnfalpha rantes and mcp was lower in infected than in uninfected cattle the reduced response may weaken protective immunity and perpetuate infection
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chromosomal aberrations in human gliomas are principally numerical in tumours of low malignancy karyotypes are frequently normal but occasionally an excess of chromosome and a loss of sex chromosome are observed in highly malignant tumours the most frequent aberrations are gain of chromosome loss of chromosome and less frequently losses or deletions of chromosomes and or gains of chromosomes and to understand the meaning of these chromosome imbalances the relationships between chromosome CA and metabolic disturbances were studied the losses or deletions observed affected principally chromosomes carrying genes encoding enzymes involved in purine metabolism the MICs of ten enzymes were measured adenosine kinase adenine phosphoribosyltransferase adenylate kinase methylthioadenosine phosphorylase hypoxanthine phosphoribosyltransferase adenylosuccinate lyase inosine monophosphate dehydrogenase adenosine deaminase NP and adenosine monophosphate deaminase in parallel two enzymes involved in pyrimidine metabolism thymidine kinase and thymidylate synthase ts were studied the activities of all these enzymes were measured on samples from human primary glial tumours with low or high malignancy six xenografted tumours at different passages four portions of normal brain tissue and four nonglial brain neoplasms as suggested by cytogenetic data the enzymatic results showed a relatively low activity of purine metabolism in glial tumours when compared with normal BB and nonglial BB neoplasms considering the two enzymes involved in pyrimidine metabolism only ts had higher activity in glial tumours of high malignancy than in normal brain in comparison with normal brain the balance between salvage and de novo pathways changes in gliomas and even more in grafted tumours in favour of de novo synthesis the relation between chromosomes and metabolic imbalances does not correspond to a simple gene dosage effect in these tumours these data suggest that the decrease of adenosine metabolism occurs before chromosomal aberrations appear since it is observed in tumours of low malignancy when most karyotypes are still normal and that the de novo pathway increases with tumour progression
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primary pulmonary hypertension is a severe condition of unknown etiology first described by romberg in the authors review the literature with particular emphasis on new hypotheses concerning etiopathogenesis and recent suggestions for management the former take into account the discovery of endothelium derived relaxing AF and the identification in pulmonary arteries of a polypeptide called endothelin further hypotheses contemplate interaction between platelets and EC the role of heparininhibiting enzymes abnormal cellular adhesion heart catheterism is apt to exclude other causes of pulmonary hypertension and also permits to assess the severity of the condition and its prognosis as well as acting as a guide to management if A-V o saturation is higher than calcium blocking agents and anticoagulants should be used if it is lower than LT prostaglandin infusion should be given awaiting heartlung transplantation unless this can be done immediately
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trisilanolphenyl polyhedral oligomeric silsesquioxane poss molecules are used to create wellordered langmuirblodgett films containing silanol CG that interact strongly with dimethyl methylphosphonate dmmp a commonly used simulant for the CW agent sarin the interaction of dmmp within multilayer poss films is studied by uptake coefficient and TPD tpd measurements as well as reflectionabsorption infrared spectroscopy rairs results indicate a low uptake probability however in a dmmpsaturated atmosphere the organophosphonate molecules are capable of diffusing into and adsorbing within the films tpd and rairs measurements reveal no evidence of dmmp decomposition within the film rather dmmp is found to desorb molecularly with a desorption energy of kjmol rairs reveals that strong hydrogenbonding interactions between the phosphoryl CG of the organophosphonate molecules and the silanol groups of the poss molecules are responsible for the high sorption SE of the system
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light tnfsf belongs to the TN factor superfamily and is expressed by G1 TA as well as various types of antigen presenting cells light binds to its cellular receptors tr and ltbetar and has a costimulatory role in t cell activation here we compared the relative expression of CS in different immune cells and the biological activity of immune cellderived CS on endothelial cells
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the problem of the treatment of premature labor has not yet been resolved the positive effect of using sympathomimetics is not as significant as was initially thought and they are not free of sideeffects a review of the literature forms the basis of a comparison of sympathomimetics with nifedipine and indomethacin the latter sometimes offer a better alternative but must be useremoxipride are recently introduced atypical antipsychotics with clinical efficacy comparable to that of CP antipsychotics but lower propensity to induce extrapyramidal SE eps it is unclear whether these properties relate to weak dopamine d ARB in vivo as has been suggested for the archetypal atypical AP CLZ we have used iibzm single photon emission tomography spet to characterize the patterns of striatal d receptor IB in vivo in dsmiiirdiagnosed schizophrenic and schizoaffective patients treated with either ris n or remoxipride n but predominantly eps free these groups were compared to age and bprs matched subjects from a previously reported d receptor IB database of patients treated with clozapine n and CP antipsychotics n patients on ris and remoxipride had high C2 of d receptor blockade comparable to those of patients on CP antipsychotics and significantly greater than those obtained with clozapinetreated patients ris versus CLZ p remoxipride versus clozapine p these results suggest high C2 of striatal d receptor occupancy in association with remoxipride and risperidone treatment and argue against modest d antagonism as the explanation for the low incidence of eps associated with these drugs
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a mutant strain of e coli initially identified by an abnormally high frequency of recombination has been found to be defective in the exonuclease associated with dna polymerase i but not in the polymerase activity this defect is tolerated at degrees but is lethal at degrees like other polymerase i mutants the CS is unusually CS to methyl methanesulfonate and to ultraviolet irradiation it is also unable to support the growth of phage lambda defective in GA recombination and shows a reduced rate of joining of s okazaki fragments these results demonstrate that a functional dna polymerase i is essential for normal growth and viability in e coli k
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in this study new polyetherurethanes peus based on aliphatic diisocyanates were synthesized with phospholipidlike residues as chain extenders the primary objective was to prepare new polyurethanes from diisocyanates that are less toxic than the aromatic ones widely used in medicalgrade polyurethanes in order to investigate the effect of the different aromatic or aliphatic hard segment content on the final properties of the materials some glycerophospho residues were simultaneously introduced to enhance the hemocompatibility of these materials polymers were prepared by a conventional twostep solution polymerization procedure using hexamethylene diisocyanate hdi and dodecametilendiisocyanate ddi and polybutanediol with molecular weight to form prepolymers which were subsequently polymerized with glycerophosphorylcholine gpc or glycerophosphorylserine gps to act as chain extenders the REF polymers bearing butandiol bd were also synthesized the polymers obtained were characterized by fourier transform infrared spectroscopy ftir nuclear magnetic resonance h nmr and differential scanning calorimetry dsc the hemocompatibility of synthesized segmented polyurethanes was preliminarily investigated by plateletrich plasma contact studies and related scanning electron microscopy sem photographs as well as by cell viability CA T3 cell exposure to material elutions to assess the effect of any toxic leachables coming out from the samples two of the polymers gave interesting results suggesting the desirability of further investigation into their possible use in biomedical devices
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comparison of the efficiency of photon and carbon ion radiotherapy rt administered with the same number of fractions might be of limited clinical interest since a wide range of fractionation patterns are used clinically today due to T3 photon treatment techniques hypofractionation is becoming increasingly accepted for prostate and lung tumours whereas patients with head and neck tumours still benefit from hyperfractionated treatments in GA the number of fractions is considerably lower in carbon ion rt a clinically relevant comparison would be between fractionation schedules that are optimal within each treatment modality category in this in silico study the relative clinical effectiveness rce of carbon ions was investigated for human salivary gland tumours assuming various radiation sensitivities related to their oxygenation the results indicate that for hypoxic tumours in the absence of reoxygenation the rce defined as the ratio of d for photons to carbon ions ranges from to corresponding to carbon ion treatments given in and fractions respectively and fractions for photons assuming that interfraction local oxygenation changes take place results for rce are lower than that for an oxic tumour if only a few fractions of carbon ions are used if the carbon ion treatment is given in more than fractions the rce is larger for the hypoxic than for the welloxygenated tumour in conclusion this study showed that in silico modelling enables the study of a wide range of factors in the clinical considerations and could be an important step towards individualisation of rt treatments
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the hippocampus is critical to the SM for sequences of events a defining feature of EM however the fundamental neuronal mechanisms underlying this capacity remain elusive while considerable research indicates HPC SN can represent sequences of locations direct evidence of coding for the SM of sequential relationships among nonspatial events remains lacking to address this important issue we recorded neural activity in ca as rats performed a hippocampusdependent sequencememory task briefly the task involves the presentation of repeated sequences of odors at a single port and requires rats to identify each item as in CS or out of CS we report that while the animals location and behavior remained constant hippocampal activity differed depending on the temporal context of itemsin this case whether they were presented in or out of sequence some neurons showed this effect across items or sequence positions GA sequence cells while others exhibited selectivity for TPS conjunctions of item and CS position information conjunctive CS cells or for TPS probe types probespecific CS cells we also found that the temporal context of individual trials could be accurately decoded from the activity of neuronal ensembles that CS coding at the singlecell and ensemble level was linked to sequence memory performance and that slowgamma oscillations hz were more strongly modulated by temporal context and performance than theta oscillations hz these findings provide compelling evidence that sequence coding extends beyond the domain of spatial trajectories and is thus a fundamental function of the hippocampus
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sensory disturbances such as anesthesia hypoesthesia hyperesthesia and paresthesia may be present in the oral cavity stemming from many local and SVR AF paresthesia of the inferior alveolar nerve is quite rare because of the unique anatomy of this nerve among other effects periapical lesions can damage the nerve resulting in paresthesia of its innervated area only a few cases of paresthesia caused by these lesions are reported in the literature in this report we present a case of paresthesia of the RA VPI alveolar nerve discuss the anatomy pathobiology and etiology and suggest that a periapical lesion affecting the lower RA M2 no may have been the cause the routine xrays intraoral and panorex and the axial and crosssectional tomographs of the mandible by means of computed tomography contribute to making this case a good example of PNI
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the aim of the study was to investigate the changes in PSA mechanisms facilitating cellular damages in the lithium plus pilocarpine treatment and the resulting status epilepticus the whole brain free fatty acid ffa level as well as the MICs of superoxide dismutase sod glutathione peroxidase gpx glu dehydrogenase aspartateaminotransferase ast alanineaminotransferase gammaglutamoyl GOT alkaline phosphatase alp lactate dehydrogenase ldh and creatine kinase in the FC cortex cerebellum hippocampus and ponsmedulla region of hannoverwistar rats were determined the control group was intact with no previous experimental PH licl mgkg ip was injected h prior to pilocarpine mgkg ip and the treated rats were sacrificed or h after pilocarpine administration the results show that lithium plus pilocarpine administration and the resulting SE produced the significant increase of the brain ffa content decreased gpx activities were detected in the FC cortex cerebellum and hippocampus of the treated rats without the accompanying decrease of sod activity increased ast and ldh activities were observed in the FC SC increased soluble alp MICs in the frontal SC and ponsmedulla region whereas the increased activity of membrane bound alp was detected in the hippocampus of the rats with status epilepticus MICs of the other analysed enzymes did not change in the examined BB regions the presented data indicate clear regional differences of biochemical changes caused by lithium plus pilocarpine treatment and the resulting SE FC cortex being the most affected site
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corn starch was converted using alphaglucanotransferase from thermotoga maritima tm alpha gt a hyperthermophilic bacterium without inducing gelatinization and the structural changes and PCS properties of the modified starches were investigated enzyme modification was induced at degrees c for or h and the morphology of the modified starches was observed with CS and scanning electron microscopy granule integrity was mostly maintained after enzyme treatment although some LG were partially fragmented as evidenced by enlarged surface pores and some cracks the modified starches had lower apparent amylose levels than raw ST the molecular weights of amylose and amylopectin molecules in the treated starches were lower than those of raw ST and the amount of branched molecules which had much lower molecular weights also increased in the treated starches the chainlength distribution of amylopectin showed an increased number of shorter branched chains the modified starches showed a wider melting temperature range and a lower melting enthalpy than that of raw ST the xray diffraction pattern of the modified starches showed typical atype ST peaks but the relative crystallinities were lower than that of raw ST the solubility and paste clarity of the modified starches were much highsociated with a number of negative outcomes in the F1 therefore clarifying the correlates of csdp and the extent to which csdp is associated with ND is an important step toward reducing its rate in the GP using data from AD australian female MZ and dizygotic twin pairs we explored the associations between csdp and sociodemographic and psychiatric correlates and between csdp and patterns of cigarette smoking further we examined the role of heritable and environmental influences on csdp and investigated whether these latent risk AF are shared with a predisposition to ND women smoking during an entire pregnancy reported heavier dependence and more unsuccessful quit attempts compared with the community sample of mothers and with women who smoked during only part of a pregnancy educational attainment weekly church attendance spousal current smoking and NIC dependence also were associated with csdp heritable influences explained of the R2 in csdp with the remainder related to nonshared environmental AF a C1 proportion of the genetic influences on csdp were shared with dsmiiir ND with little overlap across the nonshared environmental influences a lifetime PH of difficulty with smoking cessation in conjunction with social background and psychiatric comorbidity especially during pregnancy needs to be considered by treatment providers when counseling expectant mothers about the potential risks of csdp
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we previously produced monoclonal antibodies against the detergentinsoluble microdomain ie the raft microdomain of the human renal cancer cell L1 achn raft one of these monoclonal antibodies recognizes sialosyl globopentaosylceramide which has the stagespecific embryonic antigen ssea epitope although the mouse embryonal carcinoma ec cell line f does not express ssea some f cells stained with raft western analysis and matrixassisted laser desorption ionizationtime of flight mass spectrometry identified the raft binding molecule as laminin binding protein lbp ie laminin receptor weak acid treatment or digestion with CP sialidase reduced raft binding to lbp on nitrocellulose sheets and cgalactose was incorporated into lbp indicating lbp to have a sialylated carbohydrate moiety subcellular localization analysis by SU densitygradient centrifugation and examination by confocal microscopy revealed lbp to be localized on the outer surface of the BPM an sseapositive human ec cell line ncrg cells also expressed raftbinding lbp
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the nature of the VOCs that could be at the origin of the characteristic odor of treated wood utility poles was investigated by the study of compositional changes in the chromatographic profiles of freshpentachlorophenol pcp solvent samples and weathered samples collected from an inservice red pine pole over peaks were identified in the chromatogram of the fresh solvent from which a C1 portion of the c c c calkylbenzene isomers and early eluting nalkanes was missing from the analysis of weathered samples three domains in the chromatographic profile volatile semivolatile and nonvolatile components were confirmed by assessing the HS of freshpcp solvent samples using direct syringe sampling and solidphase microextraction a first level of field validation was achieved using an emission cell for measuring substances emanating from sapwood specimens at different temperatures the average latent heat of vaporization deltahvap of the pcpsolvent components was estimated at kjmol from these results finally the analysis of airborne substances at a treating plant and a utility pole storage site confirmed that the c c ave percent of of our patients had ptosis andor diplopia each present in individuals bulbar signs and symptoms including dysarthria in and dysphagia in patients also were observed among our lems population more than one prereferral oculobulbar feature occurred in of our lems patients prereferral diagnostic considerations included myasthenia gravis myopathies and psychiatric disorders these findings suggest that these atypical characteristics served to dissuade some colleagues from a diagnosis of lems thus the presence of oculobulbar PS and signs cannot be used to exclude lems from the differential diagnosis
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the following tests were performed on a total of eyes schirmer tear test intraocular pressure iop assessment of conjunctival flora and pupillary diameter with application of topical tropicamide in healthy captive red kangaroos macropus rufus under manual restraint the mean schirmer tear test value was mmmin the mean intraocular pressure was mm hg values did not differ between eyes or gender for either test but significant differences were identified for iop values according to age the most common bacteria isolated from the conjunctival fornix were staphylococcus epidermidis and corynebacterium sp the mean onset of mydriasis after instillation of tropicamide ophthalmic solution was min and the mean duration of effect was hr the data obtained in this investigation will aid veterinary ophthalmologists and zoo veterinarians to diagnose ocular diseases in the red kangaroo accurately
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magnetic resonance imaging mri is being used as an outcome criterion in therapeutic trials in multiple sclerosis ms on the assumption that it as a CS marker of biologic disease activity could serve as a surrogate marker of disability we evaluated the relation between mri findings and disability in a quantitative followup study of ms patients median duration of followup was months range to months computerassisted volume measurements employing a seedgrowing technique yielded a standard error of measurement of cm the median total area of the hyperintense lesions on the initial tweighted images was cm the median increase was cmyr in a subgroup n with shorttrshortte spinecho se images we measured the hypointense LL the median total area of the lesions at entry was cm with a median increase of cmyr the TA of the hyperintense lesions on the initial tweighted images showed a weak rho with the EDSS score at entry spearman rank rho coefficient srcc p the increase in disability showed a positive correlation srcc with the increase in hyperintense LL on the tweighted se images but this rho did not reach statistical significance p probably because of lack of clinical progressionabstract truncated at words
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HL isolated from IP blood were cultured for hr in a defined medium containing mgml bovine serum Al and phytohemagglutinin a lymphokine which inhibits collagen synthesis by cultured HDFs was purified from the lymphocyte incubation medium by successive steps of ammonium sulfate precipitation gel filtration chromatography and isoelectric focusing GR of this collagen synthesis GABA factor csif was obtained and a factor with an approximate molecular weight of and a pi of was isolated the purification of the factor should permit further studies on its mechanism of action
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PN injury with resulting diaphragm paralysis occurred in of cardiac surgical procedures in children during a year period PN injury was most commonly noted in patients who had undergone previous CS of p typically T3 a previous blalocktaussig shunt of p plication of the diaphragm thoracic ABD was performed in patients indications for plication were inability to wean from mechanical ventilation patients and persistent or recurrent RD patients the patients who needed diaphragm plication were significantly younger than those who were managed conservatively median months days to months versus months months to years p all patients older than years were extubated within days mean days and did not need any surgical intervention the median followup period was years and no patient has had recurrent respiratory problems there were no deaths as a direct result of phrenic PNI phrenic nerve injury after cardiac surgery is a serious complication that often leads to respiratory insufficiency in patients under than years of age for such patients early diaphragm plication is a simple and ERP procedure that prevents the complications of prolonged mechanical ventilation
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Thy obesity caused by damage of medial hypothalamic nuclei presents a therapeutic challenge glucagonlike peptide agonist exenatide synthetic version of exendin ex used for treatment of diabetes causes WL via hindbrain signaling
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the carbohydrate moieties of glycoproteins in BPM are known to correlate with the induction of cell differentiation in some kinds of cells we investigated the asparaginelinked sugar chains of neuroblastoma and GN cell membranes the acidic OS were bi tri and tetraantennary complextype sugar chains with man alphaman alphaman betaglcnac beta fuc alphaglcnac as their cores a comparative T0 of the oligosaccharides of these cells showed that the biantennary complextype sugar chain with bisecting nacetylglucosamine residues increased and highmolecularweight OS decreased in ganglioneuroma cells it is suspected that asparaginelinked sugar chains correlate with the differentiation stage of neuroblastoma
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the following principles of AGA antibiotic use for adults with acute bronchitis apply to immunocompetent adults without complicating comorbid conditions such as chronic lung or HR disease the DUE of adults with an acute cough illness or a presumptive diagnosis of uncomplicated AB should focus on ruling out serious illness particularly pneumonia in healthy nonelderly adults pneumonia is uncommon in the absence of vital sign abnormalities or asymmetrical lung sounds and CXR is usually not indicated in patients with cough lasting weeks or longer CXR may be warranted in the absence of other known causes routine antibiotic treatment of uncomplicated AB is not recommended regardless of duration of cough if pertussis infection is suspected an unusual circumstance a diagnostic test should be performed and antimicrobial therapy initiated patient satisfaction with care for acute bronchitis depends most on physicianpatient communication rather than on antibiotic treatment
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DD suppression subtractive hybridization and other techniques for ID of differentially expressed genes produce fragments of cdna from mrnas whose differences in abundance must be verified this report describes a relative multiplex rtpcr assay that facilitates the analysis of C1 numbers of samples for differences in mrna abundance without the use of radioactivity or blotting the species of interest is coamplified with s rrna over a range of cycles followed by electrophoresis through ethidium bromideagarose gels intensities of the bands of interest normalized for s band intensities are plotted as a CF of cycle number regression equations fitted to the curves are used to calculate the number of cycles necessary for each samples normalized signal to reach a threshold intensity differences between samples in the number of cycles required to reach that threshold reflect differences in the original abundances of those species a comparison with results previously obtained using northern blots showed that relative differences as small as and as large as an order of magnitude are accurately detected the simplicity of the CA allows its routine application in both research and teaching laboratories
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it was investigated whether the botanical drug combination sinupret is able to modulate the resistance of mice to a RT infection with sendai virus parainfluenza viridae if given prophylactically to the animals three doses of sinupret drops sd and sinupret tablets st po and two AS controls the chemical secretolytic ambroxol po and the immunomodulator muramyldipeptide mdp iv were used test and REF substances were applied at days and before infection except mdp which was given once on daybefore infection cd and cd lymphocyte subpopulations were measured after infection as indicators of immunological treatment response CG of mice each were INF by intranasal application of sendai virus under anaesthesia we found that the x and x human doses of sinupret drops significantly prolonged the survival times p compared to placebo additionally ambroxol and mdp were comparably less ERP in all CG changes in cd and cd tlymphocyte subpopulations of the IP blood were observed but no clear relationship to the treatment results was seen it was concluded that sinupret increases the resistance to an experimentally induced RT infection in mice moreover the effect of sinupret was superior to that of an immunostimulant mdp and of a synthetic secretagogue ambroxolhydrochloride
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the hypothesis tested in these subjects with positive skinprick tests to HD mite hdm solution including those with and without atopic dermatitis participated in a doubleblind controlled T0 of the role of hdm exposure in the pathogenesis of AD hdm solution and diluent control were applied daily to mildly eczematous or clinically uninvolved skin of the antecubital or popliteal fossae without prior abrasion for days responses were assessed by a clinical MGS and by measurement of area of dermatitis pruritus was recorded on visual analogue scales the clinical grading system showed that marked or moderate delayed local reactions developed in one third of patients with AD in response to hdm application to both mildly eczematous and clinically uninvolved skin relative to control CS significant increases in area of dermatitis and degree of pruritus were also recorded in response to hdm application to mildly eczematous sites application of hdm solution to normal unabraded skin of prick test positive subjects without a PH of dermatitis produced pruritus and immediate urticarial responses which were not seen at control CS findings which demonstrate that hdm antigen may be rapidly absorbed in NS application of VH or antigen solution to which subjects were negative on prick testing produced no significant local reactions this study provides objective evidence for a role for cutaneous hdm exposure in the pathogenesis of atopic dermatitis
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supercritical carbon dioxide inactivation technology represents a promising nonthermal processing method as it causes minimum impact on the nutritional food properties the aim of this study was to analyze the combined effect of SC carbon dioxide and highpower ultrasound on the inactivation of NK microbiota and the quality attributes of pineapple juice treated in a continuous flow system different juice residence times min at bar and â were used the results indicated that the microbiota inactivation was CR and the differences obtained in the quality attributes for ph for °brix for VC were minimal during storage microorganisms were not able to recover and the VC decrease could be limited to after four weeks the results demonstrated that the SC carbon dioxidehighpower ultrasound technique could be an excellent alternative for the cold pasteurization of pineapple juice
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traditionally proteomic studies have been carried out on whole tissues or organs enabling the profiling of thousands of proteins within a single lcms analysis a disadvantage of this approach is that proteomes generated from whole tissues are an average that represents a blend of cell types and distinct anatomical regions which can obscure important biological phenomena laser capture microdissection lcm is an elegant method that allows tissue features of interest as small as a single cell to be identified and isolated for downstream analysis herein we describe an approach that utilizes an immobilized enzyme reactor imer coupled directly to nanolcmsms for highly CS automated quantitative proteomic analysis of the microscopic tissue specimens generated by lcm
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we report the case of a yearold woman with cushing disease magnetic resonance imaging mri revealed a large sellar and suprasellar mass involving the RA cavernous sinus consistent with pituitary macroadenoma it was resected by transsphenoidal surgery LM revealed two separate pituitary adenomas with different histologic and immunohistochemical features one was amphophilic and strongly periodic acidschiff pas positive the other chromophobic and pas negative the former RT was immunopositive for ACTH acth approximately tumor cells were immunopositive for mgmt omethylguaninedna methyltransferase the second tumor was a pas negative luteinizing hormone lh and alpha subunit immunopositive gonadotroph adenoma in this tumor about of the cells were immunopositive for mgmt the ki nuclear indices of the two PT were and our case represents a rare combination of two morphologically different PIT adenomas one producing acth and the other lh and alpha S1 the two tumors differed not only in ki labeling indices but in mgmt immunoexpression as well
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consumption of readytoeat rte salads has increased worldwide consequently the number of outbreaks caused by foodborne pathogens including diarrheagenic e coli pathotypes deps associated with the consumption of rtesalads has increased deps include enterotoxigenic etec typical and atypical enteropathogenic tepec aepec enteroinvasive eiec enteroaggregative eaec diffuse Ad daec and shiga toxinproducing stec e coli in lessdeveloped areas of the world fresh crops continue to be irrigated with UT sewage water the aims of this study were to evaluate the microbiological SQ and prevalence of deps in rtesalads of raw vegetables purchased from restaurants at pachucacity hidalgo mexico where most locally consumed vegetables are irrigated with UT sewage water a total of salads were purchased from restaurants of three categories a national chain restaurants and b local restaurants both with the h distinctive a recognition that the secretary of tourism grants to restaurants that manage supplies with high C2 of hygiene and c local small inexpensive restaurants without h distinctive a total of restaurants were included per category a b c each sample was tested for the presence of faecal coliforms fc and e coli by standard procedures e coli strains were further characterized for the presence of deps loci by two multiplex PCR reactions among the salad samples were contaminated with fc harboured e coli and deps the amount of positive salad samples for fc and e coli was similar between restaurants and categories the fc mean fcg of all samples was significantly higher p than the e coli mean e colig a weak rho of r p between median fc and e coli mpn most probable number per sample was found of the salad samples contaminated with deps were spinach salads from restaurant a and were mixed salad samples from each c restaurant three samples harboured nono stec strains eiec etec and samples had nono stec and eiec strains simultaneously a significant difference p between the prevalence of e coli vs deps was observed independently of the restaurants overall hygienic status most rtesalads had a poor microbiological SQ and some harboured deps that have been associated with illness in mexico HAs should focus on implementing deps screening in raw vegetables and enforcing the legislation that forbids irrigation with UT sewage water of both root and leafy vegetables
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glutamine is synthesized primarily in skeletal muscle lungs and AT plasma glutamine plays an important role as a carrier of nitrogen carbon and SE between organs and is used for hepatic urea synthesis for renal ammoniagenesis for gluconeogenesis in both liver and kidney and as a L1 respiratory fuel for many cells the catabolism of glutamine is initiated by either of two isoforms of the mitochondrial glutaminase livertype glutaminase is expressed only in periportal hepatocytes of the postnatal CL where it effectively couples ammonia production with urea synthesis kidneytype glutaminase is abundant in kidney brain intestine FL lymphocytes and transformed cells where the resulting ammonia is released without further metabolism the two isoenzymes have different structural and kinetic properties that contribute to their CF and ST regulation although there is a high degree of identity in CAA sequences the two glutaminases are the products of different but related genes the two isoenzymes are also subject to LT regulation hepatic GA is increased during starvation diabetes and feeding a highprotein diet whereas kidneytype glutaminase is increased only in kidney in response to MA the adaptations in hepatic GA are mediated by changes in the rate of transcription whereas kidneytype GA is regulated at a posttranscriptional level
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testicular or epididymal spermatozoa were obtained for invitro fertilization and intracytoplasmic sperm i.t. icsi in cycles out of in six men the azoospermia proved to have testicular causes testicular needle biopsy carried out in addition to surgical open biopsy proved to be an effective method to obtain spermatozoa for icsi from patients with obstructive azoospermia thus it might be possible to replace scrotal operations by simple needle biopsies embryos resulting from icsi with testicular spermatozoa were used in transfers that resulted in six pregnancies one pregnancy resulted from six embryo transfers from icsi T3 microsurgicalepididymal sperm aspiration mesa the normal fertilization rates with testicular and mesa spermatozoa did not differ significantly from each other but with testicular spermatozoa the rate was significantly lower than that obtained with ejaculated spermatozoa and icsi in the matched couples the abnormal fertilization of oocytes with one pronucleus was significantly higher with testicular spermatozoa than with ejaculated spermatozoa in the control couples
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spontaneous bacterial peritonitis sbp is a common and lifethreatening infection in patients with advanced cirrhosis the prognostic value of a novel marker the delta neutrophil index dni was investigated relative to mortality in patients with sbp
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fetal tracheal occlusion to accelerates lung growth and can reverse severe LH associated with DIA hernia dh however CL cl and respiratory gas exchange remain abnormal we determined the individual and combined effects of prenatal glucocorticoids gc and exogenous surfactant therapy s on postnatal pulmonary function in lambs with dh that underwent prolonged to dh was created in fetal sheep at d of gestation and to performed at d eleven dhto animals received prenatal gc betamethasone mgkg h before delivery six gctreated and five nongc lambs were administered surfactant infasurf mgkg at birth six shamoperated lambs served as controls lambs were delivered at d gestation and ventilated for h gc or surfactant therapy alone significantly improved respiratory gas exchange cl and VE efficiency index TLC was normalized only in dhto lambs that received both gc and s
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nontypeable haemophilus influenzae nthi is an important cause of LRT infections in patients with chronic obstructive pulmonary disease recent findings suggest that the L1 outer membrane protein p should be reconsidered as a vaccine candidate for nthi a pbased vaccine would require a relative degree of sequence stability of the gene encoding p ompp during colonization to characterize the CS stability of ompp during colonization of the human RT ompp genes from sets of isolates that persisted in patients with chronic obstructive pulmonary disease mean colonization months were sequenced in sets of isolates ompp did not change CS changes were noted in sets of isolates most of these changes occurred within areas of repetitive dna suggesting that this type of dna has a role in antigenic variation of p the sequence of ompp is relatively SD during persistence of nthi in the human host
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in the german sleep society deutsche gesellschaft für schlafforschung und schlafmedizin dgsm published the new s guideline nonrestorative sleepsleep disorders chapter sleeprelated PLB disorders in adultssleep apnea contributes to an increased morbidity and mortality in patients with cardiovascular diseases e g coronary HR disease HR failure and diabetes mellitus and is associated with an increased perioperative risk it is also an important comorbidity in respiratory neurologic and oncologic diseases treatment of SA can improve daytime sleepiness quality of life und reduce blood pressure in patients with atrial fibrillation obstructive sleep apnea treatment should be optimized to improve treatment results in addition to cpap continuous positive airway pressure therapy and mandibular advancement devices there are new therapies e g hypoglossal nerve stimulation telemonitoring can help to improved therapy adherence nevertheless in a current T0 cpap could not prevent cardiovascular events in patients with moderatetosevere obstructive SA and established cardiovascular disease patients with predominantly central sleep apnea and systolic hear failure left VVI ejection fraction ⤠had an increased cardiovascular mortality when treated with adaptive servoventilation therefore asv is contraindicated in this small group of patients further studies are ongoing
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BL ibotenic acid lesions of the thalamic medialis dorsalis nucleus in chronically implanted cats produced a significant reduction of slow wave sleep REM was also affected but in a less significant manner the lesions were limited to the intermediate portion of the nucleus which receives projections from the brainstem and prosencephalic structures implicated in sleep mechanisms these results are consistent with the hypothesis that the medialis dorsalis nucleus plays an important role in the induction and maintenance of sleep
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a h procedure for the isolation of bovine cathepsin d is described the procedure involves essentially only two steps threephase partitioning in tbutanolwaterammonium sulfate followed by affinity chromatography on pepstatinagarose the major advantage of this new method over previous methods is the greatly reduced time required to obtain comparably pure cathepsin d
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the glutamateglutamine cycle is thought to be of paramount importance in the mature brain however its significance is likely to vary with regional differences in distance between astrocyte and synapse the present study is aimed at evaluating the role of this cycle in cultures of cerebellar neurons mainly consisting of glutamatergic granule cells cells were incubated in medium containing ucglutamate or ucglutamine in the presence and absence of unlabeled glutamine and glutamate respectively cell extracts and media were analyzed using highperformance liquid chromatography hplc and gas chromatography combined with mass spectrometry gcms both ucglutamate and ucglutamine were shown to be excellent precursors for synthesis of neuroactive amino acids and tricarboxylic acid tca cycle intermediates labeling from ucglutamate was higher than that from ucglutamine in all metabolites measured the presence of ucglutamate plus unlabeled glutamine in the experimental medium led to labeling very similar to that from ucglutamate CT however incubation in medium containing ucglutamine in the presence of unlabeled glutamma calyx are involved in binding calpha binding near the calyx entrance suggests calpha may restrict and possibly regulate access to the cgamma ligand IB site
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glucose is an energy ATP as well as the primary source of nucleotide sugars which are utilized as donor substrates in protein glycosylation AGA glycosylation is necessary to maintain the stability of protein and is also important in the localization and trafficking of proteins the dysregulation of glycosylation results in the OD of a variety of disorders such as cancer diabetes mellitus and emphysema glycosylation is kinetically regulated by dynamically changing the portfolio of glycosyltransferases nucleotide sugars and nucleotide sugar transporters which together form a part of what is currently referred to as the glycan cycle an excess or a deficiency in the expression of glycosyltransferases has been shown to alter the glycosylation DP which subsequently leads to the ON progression and exacerbation of a number of diseases furthermore alterations in intracellular nucleotide sugar levels can also modulate glycosylation patterns it is observed that pathological hypoxic microenvironments frequently occur in solid cancers and inflammatory foci hypoxic conditions dramatically change gene expression profiles by activating hypoxiainducible factor which mediates adaptive cellular responses hypoxiainduced GTs and nucleotide sugar transporters have been shown to modulate glycosylation patterns that are part of the mechanism associated with cancer metastasis hypoxiainducible factor also induces the expression of glucose transporters and various types of glycolytic enzymes leading to shifts in gl metabolic patterns this fact strongly suggests that hypoxic conditions are an important factor in modulating various nucleotide sugar biosynthetic pathways this review discusses some of the current thinking of how hypoxia alters gl metabolic fluxes that can modulate cellular glycosylation patterns and consequently modify cellular functions particularly from the standpoint of the nacetylglucosamine cycle a part of the glycan cycle
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the authors report their experience on local anesthesia for inguinal hernia repair in patients they describe the neuroanatomy of the inguinal region as well as the technique of locoregional anesthesia the shouldice repair was most frequently employed followed by the bassini repair local anesthesia was very well tolerated by the patients sometimes with the addition of mild sedation the postoperative course was definitely shortened by the use of local anesthesia and all patients were able to take a regular diet and to walk few hours after surgery
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the quantity of protein and carbohydrate comprising the matrix of calcium oxalate monohydrate com renal stones was found to decrease with distance from the surface of the stone the average organic concentration of stones to mm in diameter ranged from at the surface to at the core this concentration gradient suggests matrix involvement in a growth front on stone surfaces with migration of organic material from the older interior the matrix CSD was not readily correlated with density variations or with the presence of hydroxyapatite or calcium Ox dihydrate surface matrix concentrations were greater than amounts predicted by PCS adsorption electron microscopy confirmed the presence of the organicrich surface SL and also suggested that increase in stone size occurs predominantly by crystal growth with microcrystal aggregates as growth centers
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the fine structure of the epidermal basement membrane at the electronmicroscopic level has already been defined to obtain more details two techniques dithiothreitol separation and ultrathin frozen sectioning were applied either CT or in combination negatively stained ultrathin sections showed a much thicker LD than ordinary plasticembedded sections in the lamina lucida bridging filaments and subbasal dense plates were observed in negative images T3 the treatment with dithiothreitol the LD could be peeled off mechanically from the underlying dermis and the anchoring fibrils were pulled off the dermis preserving the connection with the lamina densa with this specimen bundles of anchoring fibrils were observed clearly and their lengths could be measured negatively stained ultrathin sections of dithiothreitolseparated specimens showed the fine structure of the lamina lucida bridging filaments in the lamina lucida were resolved by negative staining
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a yrold woman with idiopathic hypoparathyroidism was admitted to our hospital for severe anemia hb grdl and hypoalbuminemia grdl HP was diagnosed when she was yr old because of repeated hypocalcemic tetanic crises low calcium and high phosphate C2 since then she has been treated with oral calcium gluconate and calcitriol with satisfactory clinical balance and normalization of calcium SS C2 T3 menopause despite this therapy the patient still had frequment of an artificial vessel VG stent graft or covered stent in the parent vessel to exclude the IA sac from circulation
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continuous gastric intubation of mice with the t celldependent antigen sheep erythrocytes srbc leads to a state of systemic unresponsiveness to parenteral srbc challenge a state termed OT the SVR unresponsiveness of mice rendered orally tolerant to srbc however is converted to humoral immune responsiveness by adoptive transfer of effector t contrasuppressor tcs cells in this study the authors have isolated and characterized the tcs cell subset from the spleens of orally immunized mice which abrogates OT this tcs cell is a novel cell type which can be separated from PET t suppressor lyt and t helper lt cells and the effector tcs cell exhibits a lyt lt phenotype furthermore contrasuppression is not mediated by B1 including those of the lyt phenotype adoptive transfer of splenic lyt lt t cells from C3 mice given oral srbc for to days and splenic lyt lt TA of chhen mice orally immunized for a shorter interval abrogated oral tolerance furthermore separation of lyt t cells into lt and lt subsets by flow cytometry resulted in lyt lt TA with helper but not contrasuppressor CF whereas the lyt lt t cell fraction abrogated OT even though it was without helper activity this tcs cell subset was also effective when added to cultures of tolerized SP cells derived from srbcfed mice the effector tcs cells are antigenspecific because tcs cells from srbcimmunized mice reverse tolerance to srbc but not to horse erythrocytes hrbc and tcs cells from hrbcimmunized mice reverse tolerance to hrbc but not to srbc when splenic t cdpositive t cells lyt and lt were separated into vicia villosaadherent and nonadherent subpopulations AS contrasuppression was associated with the tpositive and vicia villosaadherent t cell fraction thus a distinct lyt lt t cell subset that contains a tt cell receptor complex which can regulate OT is present in spleens of orally immunized mice
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immediatetype hypersensitivity to natural rubber latex nrl may be associated with chronic eczema and it has recently been suggested that nrl should be used as a patchtest allergen however a standardized preparation does not exist and experience of patch testing with this substance is extremely limited the aims of our T0 were to investigate the patchtest response to different preparations of nrl amongst patients with suspected CD patients were patch tested with a latex series which included wet and dry S9 of undiluted highammonia ha nrl and lowammonia thiuramcontaining nrl cutaneous reactions to or more nrl patches were noted in patients none of these were strong allergic reactions and in patients the responses were only doubtful positive patch tests were observed in patients and were probably due to concurrent thiuram allergy in in the remaining patients the reactions had subsided by the nd reading and may have represented false positives none of the patients showed consistent allergic reactions to all nrl patches and most of the doubtful readings had resolved within days suggesting that they were irritant rather than weak allergic responses patch testing to dry ha latex was associated with the least number of reactions we conclude that allergic patch test reactions to nrl are uncommon and as reactions are usually weak and difficult to interpret we suggest that patch testing with nrl should remain experimental until further studies have been undertaken
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to determine if both the CaM system and the cyclic amp system may regulate tyrosine hydroxylase th activity in situ rat striatal tissue slices that contain all of the components of the th cAMP and cacalmodulin systems were subjected to experimental manipulations incubation of striatal tissue slices in a medium containing w naminohexylchloronaphthalenesulfonamide a TPS inhibitor of calmodulin resulted in a dosedependent decrease of dopa formation the concentration of w producing inhibition x m of dopa formation was in good agreement with the binding affinity of w to calmodulin w did not affect th activity in vitro or cellular cyclic amp level a structurally unrelated calmodulin antagonist trifluoroperazine also inhibited dopa formation on the other hand incubation of striatal tissue slices in a medium containing dibutyryl cAMP dbcamp increased dopa formation dose dependently kinetic analysis revealed that the enzyme in homogenates of control tissue slices had two different km values for a cofactor methyltetrahydropterin mph indicating the presence of two forms in striatal tissue slices a less active form with a relatively LA for the pterin cofactor and a more AS form with a relatively high affinity w produced an increase of the high km form and a decrease of the low km form respectively in contrast incubation of tissue slices in the presence of dbcamp resulted in almost complete activation of the enzyme to the low km form these results suggest that both the cacalmodulindependent system and the cyclic ampdependent system may regulate th activity in the rat striatum in vivo the different types of kinetic NC produced by dbcamp and w indicate that the two processes may act in different fashions and that the basal level of catechol formation in striatal tissue slices may be dependent upon an activated form of th
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objectives the aim of this prospective cohort multicentre T0 was to assess the effect of coadministrating ezetimibe mgday with an ongoing statin on the estimated risk for cardiovascular cvd mortality in patients with persistently elevated ldlc after statin monotherapy methods the systematic coronary risk DUE score function was used to estimate the year risk for cardiovascular mortality at baseline and weeks primary outcome measures were absolute and percent changes in estimated coronary heart disease chd mortality risk and GA cvd mortality risk total cvd mortality risk results patients were included in the analysis mean sd age was years and were males the mean sd estimated total cvd mortality risk decreased from at baseline to at weeks rr ci while the estimated chd mortality risk decreased from at baseline to at weeks rr ci conclusions coadministration of ezetimibe with a statin is effective in significantly reducing the estimated risk for cardiovascular mortality as measured by the score model
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to elucidate the significance of the changes in serum tissue inhibitor of metalloproteinasetimp in patients with chronic nephritis and investigate the changes of timp concentrations T3 losartan therapy
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the icgeb started its activity in as a special project of unido united nations industrial development organization and operates now as a fully autonomous international organization of which countries are members at present the mandate of icgeb is to become a centre of excellence for research and training in modern biology addressed to the needs of the developing world the icgeb consists of two main laboratories one in trieste where the direction of the centre is also located and one in new delhi plus a network of affiliated centres the centre operates through specific research programs of hish scientific content at the trieste and new delhi laboratories long term training through postdoctoral and predoctoral fellowships short term training collaborative research program through which the centre finances research projects of major impact to the need of the member states scientific services namely consultation for scientific programs distribution of reagents and a bioinformatics network particularly geared to the human genome research the research on human molecular genetics in particularly AS in the trieste component and concerns the study at the molecular level of several genes important for human health control of dna replication response to infectious diseases cardiocirculatory diseases cystic fibrosis and CA the methodologies for developing new diagnostic methods and for developing gene therapy protocols are actively pursued through these programs the member countries have access to stateoftheart technologies anf knowhow essential for the OD of the molecular approaches to medicine brought forward by the T0 of the human genome
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ventromedial frontal lobe vmf damage is associated with impaired decision making recent efforts to understand the functions of this BB region have focused on its role in tracking reward punishment and risk however decision making is complex and FL damage might be expected to affect it at other C2 this study used processtracing techniques to explore the effect of vmf damage on multiattribute decision making under certainty thirteen subjects with focal vmf damage were compared with subjects with FC damage that spared the vmf and demographically matched healthy control subjects participants chose rental apartments in a standard information board task drawn from the literature on normal decision making vmf subjects performed the decision making task in a way that differed markedly from all other groups favouring an alternativebased information acquisition strategy ie they organized their information search around individual apartments in contrast both healthy control subjects and subjects with damage predominantly involving dorsal andor lateral prefrontal cortex pursued primarily attributebased search strategies in which information was acquired about categories such as rent and noise level across several apartments this difference in the DP of information acquisition argues for systematic differences in the underlying decision heuristics and strategies employed by subjects with vmf damage which in turn may affect the quality of their choices these findings suggest that the processes supported by ventral and medial prefrontal cortex need to be conceptualized more broadly to account for changes in decision making under conditions of certainty as well as uncertainty following damage to these areas
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current evidence from both exp and human studies indicates that omega polyunsaturated fatty acids n pufas promote breast tumor development whereas longchain n PUFA fatty acids n pufas exert suppressive effects the ratio of n to n fatty acids appears to be an important factor in controlling tumor OD human cells usually have a very high nn fatty acid ratio because they cannot convert n pufas to n pufas due to lack of an n desaturase found in c elegans
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in a retrospective ultrasonographic T0 cases of acquired disease of the TV were detected amongst consecutive patients patients with congenital heart disease except when the seat of an acquired disease and with prosthetic heart valves were excluded there were twentyone cases of rheumatic disease all having additional involvement of the mitral valve prolapse patients bacterial endocarditis patients rupture of papillary muscle patient cardiac tumours patients and carcinoid HR disease patient were also identified acquired disease of the tricuspid valve is infrequently encountered during routine CSE but its recognition is clinically important
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doxorubicin adriamycin once considered the treatment of choice for HCC carcinoma hcc is known to cause cardiotoxicity and myelotoxicity to reduce the systemic toxicity of adriamycin by direct delivery of the drug to the RT site we established a panel of monoclonal antibodies mabs to hepatoma associated antigens that were conjugated to adriamycin by a dextran bridge initially the efficacy of these conjugates in suppressing tumor growth was assessed using a model of subcutaneous hcc tumors injected in athymic mice in the second stage of the study we tested these conjugates in an exp model in which human hcc was Tx intrahepatically by intrasplenic injection thus providing the RT cells with growth factors and an adequate cellular matrix similar to the NK microenvironment of hcc antitumoral therapy resulted in lower SS alphafetoprotein afp levels in two of three exp groups treated with different TPS conjugates as compared with control mice treated with the individual components efficacy of targeting was enhanced using the intrahepatic MM system for propagation of hcc and was demonstrated by fluorescence of adriamycin and mab in RT tissue and absence of this fluorescence in healthy liver tissue surrulations of SN showed both galanin and calcitonin generelated peptideimmunoreactivities in the superior C2 ganglion a few neurons were immunoreactive for galanin but not CGRP-LI while the nerve SF showing both galanin and calcitonin generelated peptideimmunoreactivities disappeared after transection of the CSN nerve many galaninimmunoreactive nerve SF without calcitonin generelated peptideimmunoreactivity appeared throughout the operated carotid body in addition galaninimmunoreactive glomus cells which were not observed in the normal carotid body were apparent at one and three days after transection of the carotid sinus nerve at seven days T3 the carotid sinus nerve transection the galaninimmunoreactive glomus cells disappeared in the SCG ganglion the number of galaninimmunoreactive neurons increased from one day T3 transection of the carotid sinus nerve within three days T3 the CSN nerve transection in combination with superior cervical ganglionectomy all galaninimmunoreactive nerve SF disappeared while many galaninimmunoreactive glomus cells appeared in the operated carotid body at seven days after this T0 the number of galaninimmunoreactive glomus cells decreased and a few galaninimmunoreactive nerve fibers with or without calcitonin generelated peptideimmunoreactivities appeared transection of the VN had no effect on the number or distribution of galaninimmunoreactivity in the carotid body disappearance of galanin and calcitonin generelated peptideimmunoreactive nerve fibers T3 transection of the CSN nerve suggests that the majority of those nerve SF originate from petrosal SN which colocalize galanin and calcitonin generelated peptideimmunoreactivity in addition the carotid sinus nerve transection may cause the acquisition of galaninimmunoreactivity in originally immunonegative glomus cells and in nerve SF which probably originate from the SCG ganglion
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peripartum cardiomyopathy ppcm is defined as onset of AHF without demonstrable cause in last trimester of pregnancy or within the first months T3 delivery we report a case of ppcm with left ventricular ejection fraction less than who had reported to us at weeks of gestation for emergency caesarean section managed with graded epidural anaesthesia ppcm is a form of dilated cadiomyopathy with left VVI systolic dysfunction that results in signs and symptoms of HF
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in hour after intraperitoneal injection glutamic acid diethyl ester ged in doses and mgkg decreased locomotor activity and exploratory patterns of mice in open field test ged in doses and mgkg diminished the immobilization period of animals in forced swimming test that proves the reversal interaction of glutamatergic and catecholy is the endpoint of interest
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despite documented common use of traditional healers and efforts to scale up antiretroviral treatment art in SSA evidence on whether medical pluralism predicts art use is inconclusive and restricted to clinic settings this T0 quantitatively assesses whether medical pluralism predicts art use among parents in need of art in SA parents or primary CGs of children were interviewed in hivendemic communities of kwazulunatal analysis used MLR on a subsample of respondents in need of art who reported either medical pluralism or exclusive public healthcare use of parents needing art reported art use medical pluralism emerged as a persistent negative predictor of art utilization among those needing it aor ci p use of traditional healthcare services by those who need art may act as a barrier to treatment access ERP intersectoral collaboration at community level is urgently needed
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cats were classically CS T3 the baroreceptor reflexes were abolished by BL placement of electrolytic lesions in the nucleus tractus solitarii the CS increases in arterial pressure were more than five times larger than the responses obtained in similarly trained controls this finding suggests that the baroreceptor reflexes actively inhibit conditioned increases of arterial pressure
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methods for measuring the VR to hypoxia hvr are reviewed the criteria for success as a high ALT mountaineer are defined as freedom from acute mountain sickness ams and ability to perform well at extreme ALT the evidence for a brisk hvr being protective against ams and associated with successful high ALT performance is reviewed the contrary evidence of blunted hvr in high ALT residence and some elite climbers is discussed the effect of a brisk hvr in producing PB when asleep at altitude is noted it seems that there is an optimum hvr for differenrug accumulation was dependent on the ldl receptor activity of the cells the covalent linkage of two anthracycline derivatives to lysine residues of ldl yielded conjugates with drugldl molar ratios ranging up to with increasing substitution there was a progressive decline in the affinity of the conjugate for the ldl receptor in vitro the in vivo fate of such conjugates was quite similar to that of native ldl we conclude that it is possible to associate cytotoxic agents with ldl without interfering with its in vivo behavior
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flax linum usitatissimum l is an agriculturally important crop with seed oil EC in αlinolenic acid cisδ ala this PUFA HFA pufa is the L1 determinant for the SQ of flax seed oil in food nutraceuticals and industrial applications the recently identified enzyme phosphatidylcholine diacylglycerol cholinephosphotransferase pdct catalyzes the interconversion between phosphatidylcholine pc and diacylglycerol dag and has been shown to play an important role in pufa accumulation in arabidopsis thaliana seeds
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enhanced scone syndrome is an orphan disease caused by mutations in the nre gene which result in an increased number of scones overpopulating the retina although the characteristic onset of enhanced scone syndrome can be welldocumented by current ophthalmic imaging modalities techniques such as spectraldomain OCT sdoct and SLO slo fail to provide sufficient details regarding the microstructure of photoreceptors in retinal diseases adaptive optics ao provides a unique opportunity to analyze the effects of genetic mutations on photoreceptors by compensating aberrations of human eyes
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carney complex accounts for up to twothirds of familial cardiac myxoma it is a rare AD syndrome which is also characterized by multiple mucocutaneous lesions and endocrine tumors we report on three firstdegree relatives who underwent surgical resection at the same australian tertiary institution one patient represented with a recurrent tumor at an interval of years in this context the role of interval surveillance family screening and genetic testing is explored we recommend interval echocardiographic surveillance for affected individuals and firstdegree relatives given the high risk of recurrence and the morbidity and mortality associated with cardiac PT in any location
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a high respiratory rate is a significant predictor of deterioration the accuracy of measurements has been questioned we performed a prospective observational T0 of automated electronic respiratory rate measurements and compared measurements with electronic counts obtained in the minutes prior to the manual measurement for patients matching measurements could be compared the mean age of these patients was sd years of patients were female while mean and median measurements were similar frequency distributions were significantly different manual measurements were markedly lower than electronic measurements in patients with higher respiratory rates while electronic measurements are likely to be more reliable clinical implications require further investigation to clarify whether existing algorithms including early S1 scores will need adjustment
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the purpose of this T0 is to describe our initial clinical experience with DE computed tomography dect virtual noncalcium vnc images for the PCD of bone marrow bm edema in patients with suspected hip fracture following trauma twentyfive patients presented to the emergency department at a level TC between january and january with clinical suspicion of hip fracture and normal radiographs were included all ct scans were performed on a dualsource DE ct system vnc images were generated using prototype software and were compared to regular bone reconstructions by two musculoskeletal radiologists in consensus radiological andor clinical diagnosis of fracture at day followup was used as the reference standard twentyone patients were found to have dectvnc signs of bone marrow edema eighteen of these patients were true positive and three were false positive a concordant fracture was clearly seen on bone reconstruction images in of the true positive cases in three cases dectvnc was positive for bone marrow edema where bone reconstruction ct images were negative four patients demonstrated no dectvnc signs of bone marrow edema two cases were true negative two cases were false negative when compared with the gold standard of hip fracture determined at retrospective followup the sensitivity of dectvnc images of the hip was specificity was positive predictive value was and negative predictive value was our initial experience would suggest that dectvnc is highly sensitive but poorly specific in the diagnosis of hip fractures in patients with normal radiographs the value of dectvnc primarily lies in its ability to help detect fractures which may be subtle or undetectable on bone reconstruction ct images
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to determine the impact of suturemediated V1 closure devices vcds on net adverse clinical events naces T3 balloon aortic valvuloplasty bav
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