text
stringlengths 10
10.1k
| source
stringclasses 1
value |
|---|---|
using direct cannulation of renal lymphatic vessels and simultaneous photography of the exposed dog kidney during urography the time schedule of variations in QL and kidney size was established the intravenous contrast i.t. produced heavy increase of renal lymph flow during the first T2 ofterials these materials are highmolecular weight but not collagen after pronase digestion labelled materials elute in the void volume of sephadex g labelled materials migrate slowly on cellulose acetate bind to several kinds of anion exchangers and elute at LIS are precipitated by cetyl pyridinium chloride cpc when mixed with carrier EDC and are not degraded by clostridium neuraminidase or streptomyces leech and bovine testicular hyaluronidases
|
MEDAL
|
the heatlabile toxin hlt of enterotoxigenic escherichia coli etec is a potent oral adjuvant we determined whether the etec hlt could be mixed with killed campylobacter to induce an immune response protective upon subsequent challenge with live pathogens mice were immunized orally three times with sonicated campylobacter with or without micrograms of etec hlt and humoral immune responses in intestinal lavage fluids measured by elisa whereas live bacteria induced strong intestinal iga responses killed bacteria did not unless etec hlt was also added the magnitude of the antibody response was dependent on the amount of antigen given the etec hlt given with bacteria also induced a potent crossreaction with cholera toxin the latter had an adjuvant effect in mice similar to that of etec hlt protection against colonization was studied in mice and rabbits in contrast to nonimmune animals those given live organisms or sonicated cells mixed with etec hlt quickly cleared homologous but not heterologous lior serotypes of campylobacter upon challenge these data show for the first time that etec hlt can potentiate an immune response to killed campylobacter that promotes a rapid clearance of live pathogens from the intestine
|
MEDAL
|
gastrointestinal PS in HX are distinctly unusual this report describes a monthold infant with a prolonged PH of lower gastrointestinal bleeding which was found to be caused by diffuse histiocytic infiltration of the colon immunopathologic and electron microscopic studies confirmed the diagnosis of histiocytosis x the differential diagnosis of lower gastrointestinal one in young children should include HX
|
MEDAL
|
the kinetics of forming all possible single base substitution PE are measured for drosophila melanogaster dna polymerase alpha and AMV reverse transcriptase seventeen CS along bacteriophage m dna are investigated so that effects of nearest neighbor base stacking on misinsertion kinetics can be evaluated polymerase alpha appears to be more error prone than reverse transcriptase polymerase alpha forms transversion mispairs at rates comparable to transition mispairs with two exceptions aa and cc are formed with significantly higher and lower efficiencies respectively reverse transcriptase forms transversions with lower efficiencies than transitions especially low being ag gg and cc for both enzymes misinsertion frequencies vary typically by fold for the same mispair in different locations misinsertion frequency can be expressed as a product of two components one based on km and the other on vmax dna polymerase alpha appears to use primarily km discrimination fold to achieve IS fidelity while reverse transcriptase shows a greater balance between km and vmax discrimination nearestneighbor base stacking interactions appear to have opposite effects on the two discrimination components the nearest neighbor influence on km is greater for correct insertions than for incorrect while the influence on vmax is greater for the incorrect base target CS that have pyrimidine as the nearest neighbor to incoming nucleotides show a higher than average misinsertion component based on km but a lower than average component based on vmax conversely target CS with nearest neighbor purines have a higher than average vmax component these results imply that nucleotide misinsertion hot spots will occur next to pyrimidines when km discrimination is dominant and next to purines when vmax discrimination is dominant when vmax and km discrimination components have similar magnitudes nearest neighbor effects tend to cancel thereby reducing the effects of base stacking on insertion error rates
|
MEDAL
|
the administration of TP tp in the femaldetermination of deoxyspergualin in plasma is described the drug was initially separated from the plasma matrix by ultrafiltration and a precolumn derivatization step was performed with naphthalenedicarboxaldehyde in the presence of sodium cyanide to yield the fluorescent nsubstituted cyanobenzfisoindole cbi derivative the cbi derivative was separated and quantitated by RP chromatography using an ods hypersil column and mobile phase of khpo mhpoacetonitrile vvv containing dodecyl sodium sulphate mm the excitation and emission wavelengths for the fluorescence detector were and nm respectively the PH was linearly related to drug concentration over the range from ng ml nm to micrograms ml microm in PB m ph spiked plasma ultrafiltrate and ultrafiltrate obtained from spiked plasma measurements could be made with a relative standard deviation of or less in PB m ph or less in spiked plasma ultrafiltrate and or less in ultrafiltrate obtained from spiked plasma
|
MEDAL
|
endless loop tachycardia or pacemakermediated tachycardia and atrioventricular desynchronization arrhythmia or repetitive nonreentrant ventriculoatrial synchrony rnrvas are two forms of reverse impulse conduction ventriculoatrial va synchrony although va synchrony can theoretically cause aggravation of HF clinical cases describing severe consequential HF are lacking
|
MEDAL
|
disruption of the circadian timing system arising from travel between time zones jet lag and rotational shift work impairs mental and physical performance and severely compromises LT health circadian disruption is more severe during adaptation to advances in local time because the circadian clock takes much longer to phase advance than delay the recent identification of rat circadian clock genes now makes it possible to examine time zone adjustments from the perspective of molecular events within the suprachiasmatic nucleus scn the principal circadian FEO current models of the clockwork posit interlocked transcriptionalposttranslational feedback loops based on the lightsensitive period per genes and the cryptochrome cry genes which are indirectly regulated by light we show that circadian cycles of mper expression in the mouse scn react rapidly to an advance in the lighting schedule whereas rhythmic mcry expression advances more slowly in parallel to the gradual resetting of the activityrest cycle in contrast during a delay in local time the mper and mcry cycles react rapidly completing the hr shift together by the second cycle in parallel with the activityrest cycle these results reveal the potential for dissociation of mper and mcry expression within the CE FEO during circadian resetting and a differential molecular response of the clock during advance and delay resetting they highlight the indirect photic regulation of mcry as a potentially ratelimiting factor in behavioral adjustment to time zone transitions
|
MEDAL
|
to document pro re nata prn prescribing practices and to identify patterns with respect to clinical characteristics and the medications prescribed
|
MEDAL
|
several types of na currents have previously been demonstrated in dorsal root ganglion drg neurons isolated from neonatal rats but their expression in AD neurons has not been studied na current properties in adult DRG drg SN of defined size class were investigated in isolated neurons maintained in primary SC using a combination of microelectrode current clamp patch voltage clamp and ICC techniques intracellular current clamp recordings identified differing relative contributions of ttxsensitive and resistant inward currents to action potential waveforms in drg neuronal populations of defined size patch Vc clamp recordings identified three distinct kinetic types of na current differentially distributed among these size classes of drg SN small drg neurons coexpress two types of na current i a rapidlyinactivating ttxsensitive fast current and ii a slowlyactivating and inactivating ttxresistant slow current the TTX-S na current in these cells was almost CR inactivated at typical resting potentials C1 cells expressed a single ttxsensitive na current identified as intermediate by its inactivation rate constants mediumsized neurons either coexpressed fast and slow current or expressed only intermediate current hH1 expression in these size classes was also measured by immunocytochemical techniques an antibody against braintype na channels ab labeled small and large SN with similar intensity these results demonstrate that three types of na currents can be detected which correlate with electrogenic properties of physiologically and anatomically distinct populations of adult rat drg neurons
|
MEDAL
|
atrial fibrillation af causes distinct changes in atrial conduction characterized as es that heralded a primary effusion lymphoma pel a rare neoplasm linked to KSHV the patient was a yearold man with AIDS syndrome who presented with fever sweats lymphadenopathy splenomegaly and refractory anaemia examination of the SP demonstrated haemophagocytosis analysis of ascites revealed pel treatment with chemotherapy and ganciclovir resulted in pCR of both conditions this case illustrates the diagnostic challenges posed by hps and supports the trial of antiviral agents in combination with chemotherapy in patients with pel
|
MEDAL
|
to create highaffinity antibodies b cells target a high rate of somatic hypermutation shm to the ig variableregion genes that encode the Fab site this mutational process requires transcription and is triggered by activationinduced cytidine deaminase aid which converts deoxycytidine to deoxyuridine mistargeting of aid to nonig genes is thought to result in the malignant transformation of b cells but the mechanism responsible for targeting shm to certain dna regions and not to others is largely unknown cisacting elements have been proposed to play a role in directing the hypermutation machinery but the motifs required for targeting shm have been difficult to identify because many of the candidate elements such as promoters or enhancers are also required for transcription of ig genes here we describe a system in cultured hybridoma cells in which transcription of the endogenous heavychain ig gene continues in the absence of the core intronic enhancer emu and its flanking matrix attachment regions mars when aid is expressed in these cells shm occurred at the wt frequency even when emu and the mars were absent together interestingly shm occurred at less than the wt frequency when emu or the mars were individually absent our results suggest that these intronic RII elements can exert a complex influence on shm that is separable from their role in regulating transcription
|
MEDAL
|
the authors studied the killer effect of nine standard strains of yeasts on samples of candida albicans isolated from the following clinical specimens oral mucosa feces bronchial wash sputum vaginal secretion urine skin lesion nail lesion and blood using this system it was possible to differentiate biotypes of CA the biotypes and were most frequently isolated most of the samples of c albicans were sensitive to at least one or more of the nine killer strains using the killer system it was possible to show that two patients harbored the same killer biotypes and respectively in different clinical specimens and another patient harbored the same biotype in bloodcultures effected in different ocasions the utilization of the killer system to differentiate types among species of pathogenic yeasts can be a useful method to establish the eventual source of infection and it is a valuable tool to control and watch for nosocomial infections caused by yeasts
|
MEDAL
|
using zincfinger nucleases zfns to cleave the chromosomal target we have achieved HF of gene targeting in the drosophila germline both local mutagenesis through nonhomologous end joining nhej and gene replacement via rat recombination hr are stimulated by target cleavage in this study we investigated the mechanisms that underlie these processes using materials for the rosy ry locus the frequency of hr dropped significantly in flies homozygous for mutations in spna rad or okr rad two components of the invasionmediated synthesisdependent strand annealing sdsa pathway when singlestrand annealing ssa was also blocked by the use of a circular donor dna hr was completely abolished this indicates that the majority of hr proceeds via sdsa with a minority mediated by ssa in flies deficient in lig dna ligase iv a component of the major nhej pathway the proportion of hr products rose significantly this indicates that most nhej products are produced in a ligdependent process when both spna and lig were mutated and a circular donor was provided the frequency of ry mutations was still high and no hr products were recovered the local mutations produced in these circumstances must have arisen through an alternative ligindependent endjoining mechanism these results show what repair pathways operate on doublestrand breaks in this gene targeting system they also demonstrate that the outcome can be biased toward gene replacement by disabling the major nhej pathway and toward simple mutagenesis by interfering with the L1 hr process
|
MEDAL
|
DHP SE impairs testis OD and CF however whether phthalates affect nonreproductive functions is not well understood to investigate this B6 mice were fed mg dinbutyl phthalate dbp in CO or VH only daily from to days T3 which tissues were collected prepubertal T0 another group was fed mgkg·d dbp from to days and then maintained untreated until weeks for determination of adult consequences of prepubertal exposure bones were assessed by microcomputed tomography and DEXA and t by ria dbp SE decreased prepubertal femur length marrow volume and mean moment of inertia AD animals exposed prepubertally to low dbp doses had lower bone mineral content and bone mineral density and less lean tissue mass than vehicletreated animals altered dynamics of the emerging leydig population were found in dayold animals fed mgkg·d dbp AD mice had VL testicular t and serum t and lh concentrations T3 prepubertal exposure and a dosedependent reduction in cytochrome p family subfamily a polypeptide insulinlike was detected in sertoli cells of adult mice po the highest dose of mgkg·d dbp prepubertally a finding supported by the induction of insulinlike expression in tm cells exposed to μm but not μm dbp we propose that lowdose dbp SE is detrimental to bone but that normal bone mineral densitybone mineral content T3 highdose dbp SE reflects changes in testicular somatic cells that confer protection to bones these findings will fuel concerns that lowdose dbp SE impacts health beyond the reproductive axis
|
MEDAL
|
this paper describes the synthesis of basefree crzrotbu cations the direct observation of nonchelated alkene and alkyne adducts of these cations and studies of the thermodynamic and dynamic properties of these novel species reaction of mtsyscpxslnwidthxslnwidthxmhxsbsbxhdzrmesbsbxmx cp chme with tertbutyl alcohol followed by phcbcf in benzene yields mtsyscpxslnwidthxslnwidthxmhxsbsbxhdzrhdfnvisfullautooitalextrsfexxhdbufnxvisfullhdmx bcf which exists as mtsyscpxslnwidthxslnwidthxmhxsbsbxhdzrhdfnvisfullautooitalextrsfexxhdbufnxvisfullhdfnvisfullautoclrfnxvisfullexexxmx solvent adducts in cdcl and cdcl solutions addition of ligands l l DDE propylene propyne butyne co phenylacetylene allene hexene cisbutene to in cdcl at degrees c results in reversible formation of mtsyscpxslnwidthxslnwidthxmhxsbsbxhdzrhdfnvisfullautooitalextrsfexxhdbufnxvisfullhdfnvisfullautolfnxvisfullexexxmx adducts nmr data for mtsyscpxslnwidthxslnwidthxmhxsbsbxhdzrhdfnvisfullautooitalextrsfexxhdbufnxvisfullhdfnvisfullautohsbsbxcbdchmefnxvisfullexexxmx indicate that the propylene coordinates unsymmetrically and is polarized with positive charge buildup at cint equilibrium constants defined by keq zrll vary in the order co propyne butyne phenylacetylene DDE allene propylene hexene cisbutene vinyl chloride loss of l from mtsyscpxslnwidthxslnwidthxmhxsbsbxhdzrhdfnvisfullautooitalextrsfexxhdbufnxvisfullhdfnvisfullautolfnxvisfullexexxmx to give appears to proceed via associative displacement by cdcl in most cases
|
MEDAL
|
the content of peripheral blood B1 b was reduced in patients of multiple myeloma mm and not in those with BMG bmg compared to normal donors p less than this observation correlated with the suppression of synthesis of normal immunoglobulin ig in mm thus cytokine MICs regulating the proliferation of normal mature B1 such as b cell stimulatory factor bsfinterleukin il b cell growth inhibitory factor bif and il in PBT and il in IP blood AC were investigated in patients with bmg n and mm n all patients of mm having a marked suppression of synthesis of all other normal ig had significantly lower levels of bsf activity and inversely higher levels of bif activity than those of normal donors however patients with bmg having no suppression of synthesis of normal ig had bsf and bif MICs similar to normal donors there was no significant difference in il and il MICs between both normal donors and bmg versus mm patients these data show that in mm altered cytokine activities correlate with suppression of synthesis of normal ig
|
MEDAL
|
there is a significant difference in the ability of human influenza a virus hn strains isolated up to and those isolated later to rescue temperaturesensitive mutants of fowl plague virus with a defect in the nucleoprotein np gene therefore the np genes of five human hn and hn influenza a virus strains isolated between and have been sequenced by comparison with previous and more recent isolates an evolutionary pathway has been established three CAA replacements were found which might be responsible for the functional difference between the ussr and the brazil strains the california hn CS isolated in had acquired by reassortment the np gene of a human hn virus circulating at about as had been previously suggested by investigations involving rnase fingerprint or hybridization techniques
|
MEDAL
|
elements within the enhancer of tlymphomagenic sl virus were examined for their contributions to transcriptional activity in t lymphocytes and nont cells a region containing two sequences rat to the enhancer core consensus CS and a sequence rat to the binding site for factor lvb was found to have the largest effect on activity evidence was obtained that suggests that the activity of this region was greater in t lymphocytes than in nont cells and that multiple elements within it were necessary for activity a second region containing sequences rat to the IB site of factor nfi and the glucocorticoid response element had about a twofold effect on transcription in both t lymphocytes and nont cell lines the twofold effect was seen whether the region containing the cores and lvb site was present or not these results indicate that the most important region for the specificity of sl enhancer activity and presumably for viral leukemogenicity comprises the core elements and the lvb site dnaproteinbinding studies demonstrated that one cellular factor sacbf bound to both core elements while a second cellular factor scbf bound to only one of them in combination with earlier studies this indicates that cells contain multiple AF that bind to the critical region
|
MEDAL
|
endogenous heparinoids impair coagulation evidenced by thrombelastography in cirrhotic patients with bacterial infection but it is not clear which glycosaminoglycans can be detected by native and heparinasemodified thrombelastography to assess the effects of different glycosaminoglycans on thrombelastography parameters and the reversibility of these effects by heparinaseimodified TEG twenty volunteers were enrolled solutions of HS DS and chondroitinsulphate were prepared at equivalent concentrations based on the composition and AC activity of danaparoid serial dilutions of each glycosaminoglycan were prepared to achieve and uml native and heparinasemodified thrombelastography antiactivated factor x activity and heparin cofactor ii activity were evaluated at each concentration a statistically significant heparinlike effect was seen with and uml HS and and uml dermatan sulphate which was CR reversed by heparinasemodified thrombelastography antiactivated FX activity was significantly increased in samples containing heparan and dermatan sulphates the heparin cofactor ii activity decreased with and uml dermatan sulphate and chondroitinsulphate but not with heparan sulphate heparan and dermatan sulphates affect haemostasis when added to whole blood in vitro detectable by native thrombelastography and completely reversed by heparinaseimodified TEG they may therefore be responsible for the heparinlike effect seen by thrombelastography in patients with cirrhosis and bacterial infection
|
MEDAL
|
there is an overgrowing emphasis on supersaturating drug delivery systems sdds with increase in number of poorly watersoluble compounds however biopharmaceutical performance from these formulations is limited by phase transformation to stable crystalline form due to their highenergy PCS form in the present T0 in vitro kinetic solubility in water and dissolution in biorelevant medium integrated with in silico physiologically based pharmacokinetic pbpk modeling was used to predict biopharmaceutical performance of sdds of poorly watersoluble compound carbamazepine cbz gastroplus⢠with T3 compartmental absorption and transit MM was used as a simulation tool for the study wherein the model was developed using physicochemical properties of cbz and disposition parameters obtained T3 intravenous administration of cbz mgkg into spraguedawley sd rats biorelevant medium was selected by screening different dissolution media for their capability to predict oral plasma concentrationtime profile of marketed formulation of cbz in vivo performance of sdds was predicted with the developed MM and compared to observed plasma concentrationtime profile obtained after p.o. of sdds into sd rats mgkg the predictions with strategy of using kinetic solubility and dissolution in the selected biorelevant medium were consistent with observed biopharmaceutical performance of sdds additionally phase transformation of cbz during gastrointestinal transit of formulations was evaluated and correlated with in vivo dissolution deconvoluted by looreigelman analysis
|
MEDAL
|
the acute pancreatitis is a disease which is unchangedly characterized by a high lethality the actual frequency of this disease is difficult to be established but its increase is probable particularly in the countries with high living standard factors of nutrition play an important role in the etiology and in the actual development and the consumption of alcoholic beverages gains increasingly significance despite modern apparative possibilities for making the diagnosis the clinical picture and proved laboratory findingsfermentative lapses disturbances of the carbohydrate metabolismare in the first place at first a conservative treatment is demanded with the preceding aim to prevent shock as well as renal and pulmonary insufficiency none of the substances which were experimentally introduced into treatment during recent years could clearly prove a superiority in contrast to conventional therapeutic principles single cases may be saved by timely T0 the problem is here the certain recognition of the BL to the surgical treatment
|
MEDAL
|
the antinociceptive effect of morphine was studied in tailflick and acetic acidinduced writhing in mice morphine effect was doserelated and mgkg sc physostigmine and mgkg ip potentiated the antinociceptive effect of morphine and the anticholinergic scopolamine mgkg ip reversed the potentiating effect of physostigmine indicating the involvement of the cholinergic system in pain coadministration of physostigmine would increase the therapeutic index of morphine thereby sparing the dose of morphine and also possibly the SE including the development of tolerance and addiction
|
MEDAL
|
we report the serum C2 of soluble interleukin receptor silr BMG beta m and interferongamma ifngamma in patients undergoing adoptive immunotherapy with ril and lymphocyteactivated killer lak cells our results indicate that ril induced a marked increase of the serum concentration of these markers although this increase varied considerably for different individuals parallel studies with the same patients also showed a marked rise in the number of ilr lymphocytes the ilrs expressed on these cells were mainly of the LA type we suggest that DUE of these markers may allow the monitoring of IS activation induced by ril in patients undergoing adoptive ril and lak cell immunotherapy
|
MEDAL
|
diogenes is a paneuropean randomized controlled dietary MI study investigating the effects of dietary protein and glycaemic index on weight regain metabolic and cardiovascular risk factors in obese and overweight families in eight EU centres the article is methodological in character and the presentation of results will be limited to baseline characteristics of the T0 populations included a total of families with at least one overweightobese parent underwent screening the parents started an initial week LCD and families with minimum one parent attaining a WL of or were randomized to one of five energy AL lowfat e diets for or months low proteinlow glycaemic index low proteinhigh glycaemic index high proteinlow glycaemic index high proteinhigh glycaemic index or control national dietary guidelines at two centres the families were provided dietary instruction plus free foods for months followed by month dietary instruction only at the remaining six centres the families received dietary instruction only for months the median WL during the lowcalorie diet was kg interquartile range kg n a total of adults and children were randomized to the month weight regain prevention phase despite L1 cultural and dietary regional differences in europe interventions addressing effects of dietary AF are feasible with a reasonable attrition
|
MEDAL
|
leishmaniasis is a protozoan disease whose diverse clinical manifestations are dependent both on the infecting species of leishmania and on the immune response of the host transmission of the disease occurs through the bite of a sand fly infected with leishmania parasites infection may be restricted to the skin in CL limited to the mucous CM in mucosal leishmaniasis or spread throughout the RES in visceral leishmaniasis or kala azar three rare clinical SCV of cutaneous leishmaniasis include diffuse CL leishmaniasis recidivans and postkalaazar dermal leishmaniasis
|
MEDAL
|
the bioactivityguided phytochemical investigation of the crude hydralcoholic extract of nectandra megapotamica was carried out using the ABD constriction test in mice which led to the isolation of three AS EDC alphaasarone galgravin and veraguensin the crude extract ebca mg kg and isolated EDC and at different doses were evaluated using the acetic acidinduced abdominal constriction test in mice carrageenaninduced paw oedema in rats and hot SP tests in rats the ebca showed a significant effect in the ABD constriction and hot plate tests but did not show activity in the rat paw oedema CA all isolated compounds displayed activity in the ABD constriction test but only compound was active in the hot SP test EDC and displayed activity in the antiinflammatory CA it was suggested that the analgesic effects obtained for ebca could be due mainly to the presence of its major compound alphaasarone
|
MEDAL
|
the crystal structure of chicken transthyretin has been solved at pm resolution by molecularreplacement techniques TTR is the protein component of the amyloid fibrils found in patients suffering from either FAP or senile systemic amyloidosis familial amyloidotic polyneuropathy is an autosomal dominant hereditary type of amyloidosis which involves transthyretin with either one or two amino acid substitutions the threedimensional structure of chicken transthyretin was determined in order to compare a nonamyloidogenic speciesvariant transthyretin with wildtype and mutant transthyretin molecules of the chickentohuman residue differences occur at positions which in human transthyretin give rise to amyloidogenic SCV although none corresponds to the AGA sidechain substitutions the model of chicken transthyretin has been refined to an rfactor of the overall fold of the protein is that of an allbeta protein compared with wildtype human TTR the avian transthyretin shows quite C1 differences in the region known to be involved in binding to retinolbinding protein it has a much shorter helical component than the human protein and some of the monomermonomer interactions are different
|
MEDAL
|
four series of omeganquinonyl amino acids were synthesized by michaellike additions the quinones include phenylthiobenzoquinone naphthoquinone methylnaphthoquinone and dichloronaphthoquinone these modified amino acids can be used for post chain assembly modifications of biologically AS MMP which target the quinonic drug to a CA damaged area the electrontransfer capabilities of the modified amino acids were probed by CV measurements the results described in this paper show that the novel nquinonyl amino acids are effective in producing SQ radicals similarly to the unconjugated quinones themselves a direct relation was found between the first reduction potentials of the quinones and their CR towards the omegaamino acids the successful generation of stable semiquinone radicals by the novel quinone derivatives is a prerequisite for the manifestation of sitedirected antitumor activity of corresponding quinonepeptide conjugates
|
MEDAL
|
a strategy for the analysis of yeast artificial chromosome yac clones that relies on PCR reaction pcr amplification of small restriction fragments from isolated yacs following adapter ligation was developed using this method termed yacadapt we have amplified several yacs from a human xqqter library and have used the pcr products for PCS mapping by somatic cell hybrid deletion analysis and fluorescent in situ hybridization one yac rs was mapped to band xq near the fragile x mutation the pcr product is an excellent renewable source of yac dna for analyses involving hybridization of yac inserts to a variety of dnarna sources
|
MEDAL
|
in the present study morphological ratio of body weight to body length weight of internal organs and haematological parameters cells count haemoglobin concentration haematocrit mean corpuscular volume mean corpuscular haemoglobin mean corpuscular haemoglobin concentration were used for monitoring potential effects of metal contaminants on the algerian mouse mus spretus the T0 also provided the opportunity to evaluate the use of morp was 2C for all benign samples the SBC ta t showed in comparison to the invasive tumours significantly lower numbers of AN cells and a higher rate of p mutations on the other hand the invasive tumours t were correlated to significantly higher proliferation rates and higher potencies for metastasizing the combination of the investigated tumour markers allowed a graduation of the biological behavior of an individual bladder cancer especially a high p mutation rate and a non aneuploid dna diagnosis were indicators for the recurrence of superficial bladder tumours invasive growth of bladder cancer was characterized by high ki proliferation and low nm protein IB
|
MEDAL
|
the extratubular efflux of periodic acidschiff pas positive material is used as a histological indicator of intrarenal reflux and correlation of the pas positive material and interstitial as well as glomerular changes were analyzed in surgical specimens consisting of renal calculi HN vesicoureteral reflux and others the incidence of extratubular efflux was apparently higher in the kidneys of HN pas positive material was seen not only in the interstitium but also flowed into the A-V and lymphatic spaces potent interstitial mononuclear cell infiltration and larger amounts of pas positive material were seen in the kidneys showing a moderate grade of hydronephrosis rather than renal calculi global GS was the most frequent glomerular lesion and segmental sclerosis and crescentic epithelial reaction in a small number of glomeruli were also noted these results imply that intrarenal reflux could play an important role in the OD of various renal tissue injuries
|
MEDAL
|
the metabolism and excretion of enrofloxacin were studied when applied as oral solution to chicken broilers for five consecutive days sixty dayold broilers were isolated within an intensively rearing poultry farm during enrofloxacin therapy mgkg per day the excreta of the isolated broilers were collected daily days T3 therapy termination for consecutive days and analyzed for the presence of ENR and its metabolites ciprofloxacin desethyleneenrofloxacin desef and desethyleneciprofloxacin descf enrofloxacin was excreted predominantly in the form of the parent compound between days and ciprofloxacin was detected in the excreta between days and whereas minor amounts of desef and descf were excreted only between days and respectively in conclusion the analysis of the excreta showed that approximately of orally applied ENR was excreted as the parent compound approximately as the main metabolite ciprofloxacin and approximately as the minor metabolites desethyleneenrofloxacin and desethyleneciprofloxacin
|
MEDAL
|
adrenomedullin adm is a multifunctional peptide regulating cardiovascular homeostasis we studied the role of adm in the pathogenesis of atherosclerosis by investigating changes in adm and its receptors calcitonin receptorlike receptor crlr and receptor activitymodifying proteins ramps in aorta of apoe mice and the effect of exogenous adm administration apoe mice were fed an atherogenic diet for weeks and apoeadm mice were additionally given subcutaneous i.v. of adm ngkgh for weeks apoe mice fed an atherogenic diet showed hyperlipidemia a large plaque area and increased vessel wall thickness the mrna expression and protein level of admadm receptors were increased in the aorta compared with cblj mice the elevated mrna level of crlr and ramps correlated positively with adm mrna level radioimmunoassay revealed a higher plasma and aorta adm content by and both p respectively in apoe mice than that in cblj mice exogenous adm significantly ameliorated dyslipidemia in apoe mice admtreated mice showed fewer aortic plaques decreased PA by p and reduced ratio of plaque area to luminal area by p and ultrasonography revealed significantly reduced intimamedia thickness of the ascending branch and ABD aorta the results suggest that AS apoe mice fed an atherogenic diet showed upregulated endogenous adm and its receptors and exogenous adm treatment ameliorated the dyslipidemia and vascular AS lesions admadm receptors might be an important protective system against atherosclerosis and could become a new target of prevention and therapy for atherosclerosis
|
MEDAL
|
confocal scanning laser ophthalmoscopy cslo enables highresolution and highcontrast imaging of the retina by employing spatial filtering for scattered CS AAR however to obtain optimized image quality one must design the cslo around scanner technology limitations and minimize the effects of ocular aberrations and imaging artifacts we describe a cslo design methodology resulting in a simple relatively inexpensive and compact lensbased cslo design optimized to balance resolution and throughput for a deg field of view fov with minimal imaging artifacts we tested the imaging capabilities of our cslo design with an exp setup from which we obtained fast and high SNR ratio snr retinal images at lower fovs we were able to visualize parafoveal cone photoreceptors and nerve fiber bundles even without the use of adaptive optics through an experiment comparing our optimized cslo design to a commercial cslo system we show that our design demonstrates a significant improvement in both image quality and resolution
|
MEDAL
|
anthracyclines and the taxanes are now used earlier in the course of therapy for MBC and increasingly as part of adjuvant treatment accordingly novel therapies are warranted including alternatives to anthracyclines and combination strategies in the setting of disease resistance to or disease progression after anthracycline and taxane therapy gemcitabine is part of this strategy the gemcitabinepaclitaxel combination has emerged as part of a new standard of ACT for advanced breast cancer based on phase iii data showing superiority of this combination over paclitaxel CT in the firstline therapy setting gemcitabine alone or in combination with paclitaxel and other agents is also undergoing evaluation in other settings including the refractory metastatic setting as part of management of patients with herpositive disease and also as part of adjuvant therapy for breast cancer
|
MEDAL
|
breathing a hyperoxic gas or o protects against ischaemiareperfusion injury in rat and mouse hearts the present study investigated how oxygen concentration and duration of hyperoxic exposure influenced cardioprotection and whether hyperoxia might induce delayed cardioprotection T3 h
|
MEDAL
|
MMPs mmps are proteolytic enzymes that are involved in a variety of physiological and pathological processes including those in cns in this T0 plasma values of mmp and mmp have been compared in clinically isolated syndrome cis and RRMS multiple sclerosis rrms patients during their acute attacks in relation to the biological activity of disease therefore we compared the mmps plasma values regarding expanded disability status scale edss progression index of disease pid acute BB lesion volume seen on magnetic resonance imaging mri and index of bloodbrain barrier bbb permeability destruction the obtained results demonstrated higher plasma values of mmps in both T0 groups than control values p no statistical significances have been detected comparing the obtained values of both enzymes between cis and rrms group p in both cis and rrms CG the patients with higher edss showed higher mmps plasma values p the mmps values were also significantly higher in both study patients with higher total number comparing to those with lower number of mri brain lesion p beyond mmp in rrms all obtained correlations between mmps and edss pid volume of mri gdenhancement brain lesions and index of bbb permeability were positive p this study demonstrates alterations of both tested mmps with closed rho with the disease biological activity although mmps are being implicated in the pathogenesis of acute neuroinflammation the mmps modulation might be useful in the future design of disease modifying therapy with the TPS target profile
|
MEDAL
|
the electrokinetic behaviour of normal erythrocytes is compared with that of trypsin NBS and tosyltreated erythrocytes reduction in the net negative charge with reduction in ionic strength of the suspending medium and also on treatment with nbromosuccinimide and trypsin is discussed using a porous nonrigid polyanion as a MM for the periphery of the cell membrane it is deduced from the equivalent binding of chloride and thiocyanate ions and the absence of any effect on treatment of CRC with tosyl chloride that normal nbromosuccinimide and trypsintreated cells are polyanionic in character reduction in EA charge on treatment with nbromosuccinimide or TR is probably not due to the removal of phosphate groups from the interface nor to physical adsorption of nbromosuccinimide or trypsin the charge reduction is probably produced by bond fission with possibly a net disappearance of carboxyl groups from the electrophoretic plane of shear either by loss from or reorientation of the membrane the loss or reorientation of material associated with these carboxyl groups does not lead to any basic NC in the character of the surface of the cell nor to any obvious structural instability the biconcave discoid form is maintained and there is no significant hemolysis of the erythrocytes even after contact with a solution of trypsin for hours
|
MEDAL
|
the presence of phosphorylcholine in pneumococcal CPSs was examined by using monoclonal antiphosphorylcholine antibody of the known capsular types of streptococcus pneumoniae types viz a f a f and a gave a positive capsular reaction quellung which could be inhibited by phosphorylcholine the CPSs of these six types therefore contain phosphorylcholine
|
MEDAL
|
a patient with familial IHP whose L1 symptoms were paroxysmal kinesigenic choreoathetosis pkc and PDC pdc was reported ct revealed marked calcification in the BG the TH and the DN of cerebellum positron emission tomographic pet T0 showed decreased glucose metabolism in the basal ganglia and TH the paroxysms were eliminated by the normalization of SS calcium level thus the paroxysms seemed to be induced by hypocalcemia which stimulated the lesions in the basal ganglia and thalamus on one occasion as in pkc and on the other occasion as in pdc concomitant occurrence of both pkc and pdc in a patient is unique and is of interest in considering the pathomechanisms of these different but related disorders
|
MEDAL
|
a reversed phase liquid chromatographictandem mass spectrometric method with simple solvent extraction and purification by solid phase SE spe has been developed for the determination of coccidiostats in milk for sample preparation matrix SP dispersion extraction by organic solvent and spe with different cartridges were also tested the EDC determined include lasalocid narasin salinomycin monensin semduramicin maduramicin robenidine decoquinate halofuginone nicarbazin and diclazuril main steps of the method are addition of acetonitrile to the milk samples centrifugation removal of matrix by spe concentration by evaporation and lcmsms determination during a min time segmented chromatographic run EDC are ionised either positively or negatively calculated recoveries range between and maximum C2 are in the range of μgkg the developed method was validated in line with the requirements of commission decision ec it is applicable for control of coccidiostat residues in milk as indicated in regulation ec
|
MEDAL
|
the relationship between muscle action and fatigue is not well understood especially in terms of potential sexspecific differences the purpose of this investigation was to determine whether a different number of repetitions could be performed on the individual muscle actions of the BP and squat in men and women ten resistancetrained men n age ± years height ± cm weight ± kg body fat ± and women n age ± years height ± cm weight ± kg body fat ± participated in this balanced and randomized withingroup T0 using of a RM over the course of visits subjects performed eccentric ecc con con or combined comb set to failure on the squat and BP differences in muscle action and sexspecific number of repetitions to failure were compared on the squat and bench press where significance was p ⤠across both exercises and sex we observed significant differences between each of the muscle actions where the number of repetitions decreased from ecc to comb to con while no sexspecific differences were observed in the squat women performed significantly more repetitions on the ecc and con muscle actions of the BP men performed more combined repetitions however indicating a greater reliance on the stretchshortening cycle different muscle actions contribute uniquely to the successful performance of a lift and fatigue these contributions appear to differ in men and women
|
MEDAL
|
in pennzoil company offered a companypaid CA awareness and screening program focusing on breast and CRCs for its employees following a lectureslide presentation employees filled out a risk assessment questionnaire those at risk for breast andor CRCs were invited for consultation mammography andor short colonoscopy overall completion rates were for mammography for the fecal occult blood test and for short colon oscopy but these rates varied by risk status moreover the same demographic and risk factors were not consistently associated with completion of the same screening procedure across risk groups based on these findings we suggest that different interventions may need to be developed depending on the risk characteristics of subgroups within the target population
|
MEDAL
|
heparin a highly sulfated glycosaminoglycan has been used as a clinical AC over years however heparininduced thrombocytopenia and thrombosis hitt is a serious side effect of heparin therapy resulting in relatively high risk of amputation and even death hitt is caused by forming of complexes between heparin and PF4 pf enoxaparin one of the most commonly used low molecular weight heparin lmwh were develsapeco and roasting on the composition and AOP of maté samples were roasted °c °c °c or °c for a fixed time min or roasted or min at a fixed temperature °c hplcuvms analysis and measures of total phenolic content tpc and antioxidant activity diphenylpicrylhydrazyl dpph inhibition and soybean oil oxidation were used to analyse phytochemicals there were significant differences p anovatukey in phenolics between ms and msl samples following certain processing steps but principal component analysis pca indicated no distinction between ms and msl samples suggesting that light intensity had no effect on phenolics caffeine increased significantly p in ms samples temperature was more important than time since pyrolysis of phenolics was more intense at °c
|
MEDAL
|
a yearold symptomatic woman with possible AD williamss syndrome mental retardation elfin facies and supravalvular aortic stenosis svas is documented this patient displayed many unique features in addition to the severe svas peak systolic gradient mmhg there was an associated persistent left SVC draining into the CS MVP barlows syndrome and complete right bundlebranch block on ecg IP pulmonary i.a. stenosis was absent mmode echocardiograhy in the AD with svas is described for the first time in the literature as is the use of the degrees crosssectional scan both these noninvasive procedures proved of value in the diagnosis the degree of subendocardial ischaemia as determined by the EVR was calculated and the possible mechanisms producing angina pectoris with PD hypertension in this condition are discussed the literature on associated mitral valve CA in svas is reviewed a successful surgical result was obtained the various forms of T0 are outlined
|
MEDAL
|
in typical cases of munchausen by proxy maltreatment a mother feigns or produces illness in her child her primary goal is to accrue emotional gratification and no mental disorder better accounts for the SMB we present the first published case in which the principal manufactured ailment was celiac sprue in addition a panoply of other ailments ranging from seizures to behavioral CA was reported the case is also very unusual in the involvement of the PWS grandmother and to a lesser extent the paternal grandfather as the perpetrators although definitive intervention to protect the child occurred only after years had passed multidisciplinary teamwork ultimately resulted in a successful outcome for the child who is now doing well
|
MEDAL
|
ceruminous glands are modified apocrine PG confined to the skin lining of the cartilaginous part of the external auditory meatus PT arising from these PG are rare controversy exists regarding the term ceruminoma actually this neoplasia should be classified as adenoma adenocarcinoma adenoid cystic carcinoma and pleomorphic ceruminous adenoma we report a yearold woman first seen at santa casa of belo horizonte in presenting with HA nausea vertigo hearing loss and tinnitus on the RA for the past two years ct scan showed a RT eroding cartilaginous and bony limits with intracranial invasion she was submitted to multidisciplinary treatment with surgery followed by radiotherapy cg histology showed a ceruminous adenoid cystic carcinoma the patient manifested a right peripheral facial palsy and had no REC of the previous deficits after one year from surgery she is clinically SD
|
MEDAL
|
the correct diagnosis of asthma is usually easily made and most patients with asthma respond to therapy approximately of patients with asthma however have disease that is difficult to control despite taking maximal doses of inhaled medications patients with therapyresistant or difficulttocontrol asthma require a rigorous and systematic approach to their diagnosis and treatment the first step is evaluation and testing directed at determining that asthma is the correct diagnosis many diseases mimic asthma and these alternate diagnoses should be considered the second step is to identify and eliminate triggers that worsen asthma cigarette smoking occupational exposures and AR contribute to worsening disease most patients with difficult asthma require treatment with highdose inhaled corticosteroids and longacting inhaled betaagonists despite maximal inhaled therapy these patients will require either frequent bursts or chronic daily therapy with oral corticosteroids these patients may have resistant inflammation with a persistent inflammatory state numerous studies also suggest that compliance with asthma therapy is poor ACT with inhaled corticosteroids and LA betaagonist in a single inhaler may improve patient compliance in selected patients additional therapy with leukotriene modifiers or antiige antibody can result in improved asthma control and may allow tapering of corticosteroids use of methotrexate is not justified based on current data emerging evidence suggests that different phenotypes of difficult or therapyresistant asthma exist recognition of these subgroups allows tailored therapy and prevents overmedication in an attempt to normalize lung CF in patients with irreversible airflow one
|
MEDAL
|
the fast dotenzyme linked immunosorbent assay fdelisa was used as a field applicable tool for rapid diagnosis of schistosomiasis seven hundreds faecal specimens were parasitologically examined for PCD of s mansoni eggs and other parasitic infection FEC was done for INF patients rectal biopsies were taken from individuals with no s mansoni egg in their stool where it was used as a golden standard for diagnosis of schistosomiasis crossreactivity with other parasites was studied SS samples were tested by elisa technique for PCD of human igg antischistosomal antibodies seven hundreds urine samples s mansoni infected patients and HI were tested by fdelisa for detection of a schistosomal antigen excreted in urine using brlf mouse monoclonal antibody fdelisa results were compared with elisa detecting antischistosomal igg and stool analysis where it showed highest efficiency compared with and for elisa and stool analysis respectively the sensitivity of fdelisa was high ranging from in the four different clinical stages of schistosomiasis simple intestinal hepatosplenomegaly shrunken CL splenomegaly and shrunken liversplenomegaly ascites fdelisa was highly CS detecting infection cases with eggsgm faeces and its specificity was the antigen was characterized as a protein with a molecular weight of kda using western blot technique
|
MEDAL
|
by the character of the sensitivity of uvirradiation to nmethylnnitronnitrosoguanidine bromuracil mitomycin c crystalviolet and by the capacity to restore phage injuries the a and mutants were referred to the uvrhcr mutantto the uvrhcr and mutantto lon TT as a result of uv irradiation the exp strains formed heteromorphic forms of bacteria spindleshaped filamentous cells were sensitive to the action of static electrical field of VHF this pointing to disturbance of cellular CM structure in these mutants
|
MEDAL
|
PD pd or the dose outside the geometrical boundaries of the radiation field is of clinical importance when anatomical structures with low dose tolerances might be involved it is the aim of this study is to estimate the pd on linear accelerators on different wedge systems without multileaf collimator mlc measurements were performed on a dual energy linear accelerator equipped with tertiary mlc and enhanced dynamic wedge edw measurements were made using an ionization chamber embedded in a radiation field analyser rfa with the AA collimator and mlc setting of x x x and x cm and with the mlc fully retracted the effects of ssd on pd were measured at three ssds of and cm for the irradiation fields of x x x and x cm and the effects of the three different wedges upper wedge lower wedge and enhanced dynamic wedge on pd were measured for ° wedges with field size of x cm data were taken from cm to cm away from the field edge results show that due to tertiary mlc pd can be reduced by means of a factor of two to three at certain distance from the edge of the field compared with tg data in between the wedges the pd was less for the edw when compared with the upper and lower physical wedges we conclude that the reduction in pd is significant in reducing or eliminating the need for external peripheral shielding to reduce the dose on affected critical organs
|
MEDAL
|
plant lipoxygenases loxs ec catalyse the oxygenation of PUFA fatty acids LA and αlinolenic acid n and are involved in processes such as stress responses and development depending on the regiospecificity of a lox the incorporation of molecular oxygen leads to formation of or HFA hydroperoxides which are used by lox itself as well as by members of at least six different enzyme families to form a series of biologically active molecules collectively called oxylipins the best characterised oxylipins are the jasmonates jasmonic acid ja an furthermore the denaturing gradient gel PACE was conducted to reveal the difference in methanogenic diversity phylogenetic analysis showed that the sequences obtained were divided into three CG affiliated to the genus of methanobrevibacter methanocorpusculum and an unidentified methanogeniclike group of these sequences the predominant diversity from the genus of methanobrevibacter and the unidentified methanogeniclike archaeons in the rumen was found to be significantly induced by the high roughage diet implying that the variation of diversity at the species or strain level might have an effect on methane emissions from the rumen further analysis showed that five methangenic sequences from the rumen were possibly associated with the differential methane emissions
|
MEDAL
|
disease simulation models can be a valuable tool for planning a response to exotic disease incursions as they provide a fast lowcost mechanism for identifying the likely outcomes of a range of outbreak scenarios and disease control strategies to use these tools effectively and with confidence decisionmakers must understand the simplifications and framing assumptions that underlie a models structure sensitivity analysis the analytical process of identifying which input variables are the key drivers of the models SO is a crucial process in developing this understanding this paper describes the application of a samplingbased sensitivity analysis to the new zealand standard model nzsm this MM is a parameter set developed for the interspread plus MM platform to allow the exploration of different outbreak scenarios for an epidemic of foot and mouth disease in new zealand based on iterations of the nzsm run for a simulation period of days settings related to farmtosaleyard movements and the PCD of disease during the AS surveillance phase of the epidemic had the greatest influence on the predicted number of infected premises a small number of counterintuitive findings indicated Az of MM design implementation andor parameterisation that should be investigated further a potentially useful result from this work would be information to aid the grouping or beta of noninfluential model settings this would go some way towards reducing the overall complexity of the nzsm while still allowing it to remain fit for purpose
|
MEDAL
|
hepatitis c virus hcv infection is dependent on lipid metabolism hepatocyte steatosis occurs frequently in hcv infection but the relationship between steatosis and hcv life cycle is unclear we showed that hcv induces steatosis via the downregulation of the phosphatase and tensin homolog deleted on chromosome pten we here investigated how pten may affect hcv production
|
MEDAL
|
GSD in early gestation is important for fetuses at risk for xlinked disorders or congenital adrenal hyperplasia one hundred and seventy consecutive samples of amniocytes were collected between the th and st gestational week seven women received early amniocentesis before the th week fetal sex was determined by amplification of yspecific dna fragments within five hours all results of the PCR reaction except for one falsenegative were compatible with cytogenetic analyses polymerase chain reaction of amniocyte dna provides a rapid technique for GSD in early gestation with high specificity and sensitivity
|
MEDAL
|
the computerized tomography findings in cases of traumatic renal artery occlusion are reviewed both patients had a nonfunctioning normalsized kidney with minimal or no CE based on a review of cases of renal trauma at our institution these findings appear to be TPS for occlusion of the renal pedicle
|
MEDAL
|
using an in vivo model of erectile activity the effects of sildenafil were studied in mice lacking neuronal or EC nitric oxide synthase nnos and enos respectively under PB anaesthesia intracavernous pressure icp and mean arterial pressure map were monitored continuously in wildtype nnos and enos mice the magnitude of erectile activity was quantified as the ratio of icp to map no differences in basal icp or map were observed amongst wildtype enos and nnos mice electrical stimulation of the CN escn v hz ms s evoked increases in icp and icpmap as well as penile tumescence responses to escn were reduced in nnos but not in enos mice lname mg kg iv significantly increased map and att erectile responses in both wildtype and enos mice sildenafil mg kg iv augmented electricallyevoked erectile activity in a voltagedependent manner in wildtype mice and facilitated erectile responses in enos mice by contrast sildenafil failed to augment the diminished erectile responses in mice lacking the nnos isoform these data reveal the relative importance of nnos compared to enos as the critical nos isoform in the control of erectile function and illustrate that the nnos isoform is required for sildenafilinduced facilitation of erectile responses in vivo in mice
|
MEDAL
|
cathepsins b and l implicated in the progression of malignant tumors are regulated by a family of endogenous inhibitors referred to as the cystatins cystatin m was identified by DD as downregulated gene in MBC cells however this finding has yet to be confirmed in clinical breast cancer specimens our objective is to examine the expression levels of cystatins c m and cathepsins b and l mrna in BCCs isolated by laser capture microdissection the mrna and protein C2 of cat b l and cystatin c and m in breast cancer specimens were determined utilizing laser capture microdissectionrtpcr WB and immunohistochemical methods expression levels of either cystatin m or c were not significantly different between CL nodepositive and negative breast carcinomas increased expression levels of both cystatin m and c correlated significantly with larger tumor size cystatin m mrna was detected by in situ hybridization in both primary and metastatic BCCs our findings are at variance with a previous report proposing a metastasis suppressive function for cystatin m therefore additional studies in a larger series with adequate followup are necessary to elucidate the biologic significance of cystatin m expression in breast cancer metastasis
|
MEDAL
|
the nature of the replacement of neanderthal by anatomically and behaviorally modern populations in europe is currently a topic of lively debate in human evolution in an earlier paper gravina b mellars p bronk ramsey c nature we published a series of 14C accelerator MS measurements for the site of châtelperron in CE france which had been claimed to show a clear interstratification of successive C2 of neanderthal and modern human occupation on the basis of excavations carried out by henri delporte in the s this interpretation has recently been challenged by zilhão and colleagues zilhão j derrico f bordes jg lenoble a texier jp rigaud jp proc natl acad sci usa who suggest that the deposits excavated in the s consisted largely if not entirely of the unstratified backdirt of the earlier th century excavations on the site we show here that the excavation backdirt interpretation for the châtelperron stratigraphy can be refuted from many different aspects of the stratigraphic radiocarbon and archaeological evidence we reassess the significance of this site for current models of the coexistence and interactions between neanderthal and anatomically modern populations in western europe
|
MEDAL
|
human colorectal cancers crcs display a C1 number of genetic and epigenetic alterations some of which are causally involved in tumorigenesis drivers and others that have little PET impact passengers to help distinguish between these two classes of alterations we used a transposonbased genetic screen in mice to identify candidate genes for crc mice harboring mutagenic sleeping beauty sb transposons were crossed with mice expressing sb transposase in gastrointestinal tract epithelium most of the offspring developed intestinal lesions including intraepithelial neoplasia adenomas and adenocarcinomas analysis of over transposon insertions identified candidate crc genes of which are mutated andor dysregulated in human crc and thus are most likely to drive tumorigenesis these genes include apc pten and smad the screen also identified candidate genes that had not previously been implicated in crc including poli ptprk and rspo
|
MEDAL
|
hydroxyphenylbenzimidazole derivatives xhbis modified by various substituents x xh ch oh och no nhcoch nh nch were synthesized and their fluorescent behaviors and equilibriums in AQ solution were studied strong fluorescence attributed to the tautomer emission was observed in AQ solution at ph the fluorescence intensities of the xhbis were enhanced selectively by addition of zn but not by addition of na k mg ca mn fe co ni and cu additionally the ERP ratiometric fluorescence response to zn addition was observed in nhhbi and nmehbi the phtitration and speciation studies proved that the xhbis have two or three protonation equilibriums and one complexation equilibrium corresponding to the formation of the znxhbi complex further structural studies using D2 xray absorption fine structure analyses and density functional theory calculations identified the dominant zn species as the znhbiho complex in aqueous solution based on the substituent effect on the fluorescendol discontinuation
|
MEDAL
|
in pursuit of a single molecule with potential applications for both in vitro fluorescence microscopy and in vivo pet imaging novel targeted gagaprotoporphyrin ix ppix probes were developed optical analysis FM and radiolabeling with gallium were performed to confirm potential applications the use of a targetedppix ring in conjunction with ga or ga eliminates the need for the attachment of multiple imaging tags allowing for either in vitro fluorescentbased DUE or in vivo nuclearbased imaging
|
MEDAL
|
quantification of platelet angiotensin ii aii receptors has been suggested as a screening test for pregnancyinduced hypertension pih saturation analysis is too technically demanding to screen the requisite numbers and although a simplified method has been proposed it has not been validated against saturation this T0 sought to compare the two methods PLT aii receptor number was determined by saturation analysis and from the specific binding at each of three radioligand concentrations the two methods were compared by rho and by plotting their differences against their means to determine limits of PA significant correlations were observed at all three radioligand concentrations however none of the regression lines approached the L1 of equality there was increasing scatter as receptor number increased and confidence intervals were too C1 to be clinically useful from this study it is concluded that the simplified method for estimating platelet aii receptor number is inaccurate and caution is urged in its use as a screening test for pih
|
MEDAL
|
the pathology of many neurodegenerative diseases is characterized by the accumulation of misfolded and aggregated proteins in various cell types and regional substructures throughout the CE and peripheral nervous systems the accumulation of these aggregated proteins signals dysfunction of cellular protein homeostatic mechanisms such as the ubiquitinproteasome system autophagy and the chaperone network although there are several published studies in which transcriptional profiling has been used to examine mRNA in various tissues including tissues of neurodegenerative disease models there has not been a report that focuses exclusively on expression of the chaperone network in the present study we used the allen BB C1 online DB to analyze chaperone expression C2 this DB utilizes a quantitative in situ hybridization approach and provides data on chaperone genes within many substructures of the adult mouse brain we determined that of these chaperone genes are expressed at some level surprisingly relatively few genes only showed significant variations in levels of mrna across different substructures of the brain the greatest degree of R2 was exhibited by genes of the dnaj cochaperone TPR repeat and the hsph families our analysis provides a valuable resource towards determining how variations in chaperone mRNA may modulate the vulnerability of TPS neuronal populations of rat brain
|
MEDAL
|
we recorded the MICs of SN in the lateral surface of the posterior inferior temporal cortex pit of hemispheres of monkeys performing a visual fixation task we characterized the color and shape selectivities of each neuron mapped its receptive field rf and studied rmacological properties of the two receptors expressed in transfected cells as assessed using hht IB assays unlike the rat htb receptor which is pharmacologically dimorphic with respect to its human homologue the rat htd receptor has an almost MZ profile compared to the human htd receptor cyanopindolol and propranolol are more than fold selective for the rat htb receptor over the rat htd receptor while mianserin is more than fold selective for the rat htd receptor the rat htd receptor is expressed in cells of the dorsal raphe and thus may serve as an additional type of serotonin autoreceptor this constitutes the first unambiguous characterization of a htd receptor in rat and demonstrates its relationship to the htb receptor in rat as well as to the htd and htb receptors in human
|
MEDAL
|
a patient with an extensive necrotizing infection of the endopelvic fascia is presented cultures and tissue sections revealed the organisms responsible to be a mixed infection of bacteroides staphylococcus and streptococcus hypertension and diabetic vascular disease were felt to contribute to the initiation of the disease process in this unusual site
|
MEDAL
|
recordings of TI were consecutively taken in normal subjects age years mean two times during the same day during the following day and after month the following parameters were evaluated heart rate systolic blood pressure diastolic blood pressure left VVI ejection time preejection time baseline IMP dz P2 qdzdt interval and heather index data were processed in order to quantify the different source of R2 subjects or biological R2 days beats total variability was expressed in absolute units as coefficient of R2 results were as follows qdzdt interval had the lowest coefficient of R2 inter subject was the largest source of R2 for all the indices showed ranging from to on the contrary intraindividual R2 was moderate racing from to for various impedance measurements in conclusion thoracic electrical impedance measurements showed good reproducibility especially when successive measurements were compared in a same subject
|
MEDAL
|
micrornas mirs regulate inflammation and bmp antagonists thus they have potential uses as therapeutic reagents however the molecular CF of mirc in modulating proinflammatory and bone metabolic mediators and osteogenic differentiation is not known after mirc was transduced into a HEPM hepm a cell line of preosteoblasts using LVs the resulting mirc overexpression increased osteogenic differentiation biomarkers including osteocalcin ocn transcripts and calcium content mirc expression also downregulated interleukin il il and chemokine cc motif ligand ccl under lipopolysaccharide lps stimulation and increased OPG opg in these cells mirc directly regulates the expression of il il and ccl transcripts by IB to their utrs a plasmidbased mirc inhibitor effectively reduces their IB MICs additionally mirc delivered using polyethylenimine pei nanoparticles effectively inhibits il il and ccl in primary human periodontal ligament fibroblasts and increases the biomarkers of osteogenic differentiation in human bone marrow mesenchymal stem cells mscs including calcium content alp and runx these data demonstrate that mirc represses il il and ccl and improves osteogenic differentiation mirc may pothe most abundant phenolics found in the human diet of these the monocaffeoylquinic acids are the predominant phenolics found in fruits such as apples and pears and products derived from them in this research a comprehensive T0 of the electrospray ionization esi tandem MS msms dissociation behavior of the three most common monocaffeoylquinic acids namely ocaffeoylquinic acid cqa ocaffeoylquinic acid cqa and ocaffeoylquinic acid cqa were determined using both positive and negative ionization all proposed structures of the observed product ions were confirmed with secondgeneration ms experiments similarities and differences between the dissociation pathways in the positive and negative ion modes are discussed confirming the proposed structures and the established msms fingerprints msms dissociation was primarily driven via the cleavage of the ester bond linking the quinic acid moiety to the caffeic acid moiety within tested molecules despite being structural isomers with the same mz values and dissociation behaviors the msms data in the negative ion mode was able to differentiate the three isomers based on ion intensity for the L1 product ions observed at mz and this differentiation was consistent among various ms instruments in addition esi coupled with highfield asymmetric waveform ion mobility spectrometrymass spectrometry esifaimsms was employed for the separation of these compounds for the first time by combining msms data and differential ion mobility a method for the separation and ID of monocaffeoylquinic in applepear juice samples was developed with a run time of less than min it is envisaged that this methodology could be used to identify pure juices based on their chlorogenic acid profile ie metabolomics and could also be used to detect juicetojuice adulteration eg apple juice addition to pear juice
|
MEDAL
|
proponents of the modified RAST test rast for allergy diagnosis claim an advantage over ST in that immunotherapy may often be safely begun at higher initial concentrations and maintenance achieved with fewer i.v. than with conventional schedules the resulting fewer visits would offset the greater initial cost and the delay in obtaining results the most important allergen for consecutive patients with SAR beginning immunotherapy was determined from the PH skin testing and aeroallergen surveys a modified rast was performed for these allergens thirtynine patients had a modified rast class most severe patients were class and one patient was class since the initial concentration and total number of i.v. projected to reach maintenance vary little between our conventional schedule wv i.v. and thr epithelium containing goblet cells are described both lesions were found in the SAS between the vertebrobasilar arterial system and the BS one cyst was an incidental finding in a patient who died of orbital phycomycosis the cyst was filled with clear mucinous material the second cyst presented as a mass adjacent to the BS in a woman who had progressive brainstem dysfunction this lesion showed transition from pseudostratified ciliated columnar epithelium with goblet cells to papillary stratified squamous epithelium histologic features essentially MZ to those of squamous papillomas of the nasal cavity this lesion was filled with squamous debris the proposed origin of these lesions is discussed
|
MEDAL
|
a yearold woman developed AM hemorrhage and was positive for the myeloperoxidase ANCA mpoanca she was diagnosed as having probable microscopic polyangiitis mpa in accordance with the japanese criteria BB computed tomographic and magnetic resonance imaging mri scans revealed asymptomatic chronic CH and VWM lesions she also later developed thalamic infarction GE tweighted mri showed cerebral microbleeds cerebral microbleeds in our patient represent bleedingprone necrotizing vasculitis which is a characteristic pathologic feature of mpa although cerebrovascular disease is not major complication of mpa CH occurs more frequently than ICM infarction and it can be critical and fatal BB mris including gradient echo tweighted imaging should be performed on patients diagnosed as or suspected of having mpa to assess the risk of CBF hemorrhage
|
MEDAL
|
to study the effects of oxytocin on both spontaneous phasic contractions and k outward currents ik of the socalled nontarget smooth muscle cells physiological concentrations of oxytocin ranging between moll and moll were applied to smooth muscle preparations and single voltageclamped cells isolated from the circular layer of the guineapig gastric antrum oxytocin moll to moll suppressed in a dosedependent manner the tetrodotoxin and atropineresistant spontaneous phasic contractions and shifted rightward the doseresponse curves of moll charybdotoxin and moll bacl in cells with preloaded intracellular ca stores oxytocin moll to moll caused a dosedependent activation of the charybdotoxinblockable noninactivating component of ik iksl of single voltageclamped cells which was accompanied by hyperpolarization of the cell CM lysvasopressin and AVP failed to mimic the effects of oxytocin on both contraction and k currents further the oxytocininduced activation of iksl was effectively antagonized by x moll u or x moll nitrocarboxyphenyl nndiphenylcarbamate inhibitors of the CM phospholipase c as well as by intracellularly applied heparin selective inhibitor of IP3 ipinduced ca RyRs in cells incubated in the absence of ca entry throughout the study oxytocin moll caused a slight and transient increase of iksl amplitudes neither ryanodine moll nor cyclopiazonic acid moll were able to restore the ikactivating effect of oxytocin in these cells the data obtained suggest i that selective oxytocin receptors are present on the CM of guineapig antral smooth muscle cells ii that the oxytocinrelated relaxation may result from the activation of casensitive k conductivity via activation of ipinduced release of ca from the submembrane located cisternae of the sarcoplasmic reticulum ca stores and iii in turn this evokes a noninactivating component of ik hyperpolarizing the CM
|
MEDAL
|
percutaneous iliosacral screw IS requires substantial experience and detailed anatomical knowledge to find the proper entry point and trajectory even with the use of a navigation system our hypothesis was that threedimensional d fluoroscopic navigation combined with a preoperative computed tomography ctbased plan could enable surgeons to perform safe and reliable iliosacral screw insertion the purpose of the current T0 is twofold to demonstrate the navigation accuracy for sacral fractures and sacroiliac dislocations on widely displaced CAD pelves and to report the technical and clinical aspects of percutaneous iliosacral screw insertion using the ctdfluoroscopy matching navigation system
|
MEDAL
|
the purpose of this T0 is to assess predictors of inadequate endometrial cavity thickness ect defined as mm in frozen embryo transfer fet cycles
|
MEDAL
|
monovalent ion stimulated atpase activity from oat avena sativa roots has been found to be associated with various MF cell wall mitochondrial and microsomal of oat roots the atpase requires mg or mn but is further stimulated by k and other monovalent ions the monovalent ions are ineffective in the absence of the divalent activating cation the atpase has been described with respect to monovalent ion specificity temperature ph substrate specificity and mg and k concentrations it was further shown that oligomycin inhibits a part of the total atpase activity and on the basis of the oligomycin sensitivity it appears that at least membrane associated atpases are being measured the mitochondrial fraction is most sensitive to oligomycin and the microsomal fraction is least sensitive to oligomycin the oligomycin insensitive atpase appears to be stimulated more by k than the oligomycin CS atpaseit was further shown that per gram fresh weight of roots approximately to mumoles of k were absorbed per mumole of k stimulated atp hydrolysis this result was obtained for a variety of k concentrations and was taken to mean that sufficient membrane associated atpase exists to account for k transport in the oat roots
|
MEDAL
|
doublestranded endonuclease activity was demonstrated for the first time in the excretorysecretory es products of a parasitic nematode trichinella spiralis which can reorganize host muscle cells the endonuclease introduced doublestranded breaks to the native dna the es doublestranded endonucleases was sequence nonspecific with a ph optimum below and required divalent cations as a cofactor its activity was inhibited by the zn ion it was detected mainly in the es products of the infectivestage larvae of t spiralis collected at degrees c and was present in much smaller amounts in samples collected at degrees c and in the products of t pseudospiralis a nonencapsulated species the activity of endonuclease was blocked by antibodies against es products zymographic analysis showed that the endonuclease activity was associated with at least three moleciated with chronic infections
|
MEDAL
|
a yearold neutered male domestic shorthair cat was presented to the veterinary medical teaching hospital at the university of georgia for further DUE of a suspected osteolytic lesion of the left th rib results of a cbc and biochemistry profile revealed mild nonregenerative anemia hyperproteinemia hyperglobulinemia and hypercalcemia serum protein electrophoresis was consistent with a monoclonal gammopathy marked PU with an increased urine protein to creatinine ratio was found cytologic examination of the CL spleen and bone marrow revealed numerous PCs many of which were erythrophagocytic within the bone marrow the plasma cells contained phagocytosed metarubricytes in addition to phagocytosed erythrocytes a diagnosis of erythrophagocytic multiple myeloma was made and treatment with prednisone and melphalan was begun four weeks after presentation the cat was euthanized due to clinical deterioration a complete necropsy was performed the distal onethird of the left th rib was completely absent histologically there was no evidence for osteolysis or neoplastic cells in the remaining portion of the rib however large sheets of PCs were found infiltrating the spleen and bone marrow moderate erythrophagocytosis by the PCs was observed in both organs the plasma cells including the erythrophagocytic cells were positive for cdalpha by immunohistochemical staining erythrophagocytosis by PCs as a cause of anemia is uncommon in people with multiple myeloma and is rare in animals to our knowledge this is the first report of erythrophagocytic plasma cells in a cat with multiple myeloma
|
MEDAL
|
the total arylsulphatase activity and the relative activities of lysosomal arylsulphatases a and b were measured in the CL of control rats and rats subjected to treatments that provoke hepatic autophagocytosis the total liver arylsulphatase activities were increased in starved and starved glucagontreated rats but not in shamoperated and hepatectomized rats arylsulphatases a and b in the mitochondriallysosomal ml fraction were separated by polyacrylamidegel PACE at ph they were made visible by incubating the gels with pnitrocatechol sulphate as substrate and measured by quantitative densitometry in UT controls arylsulphatases a and b comprised and of the total arylsulphatase activity respectively the arylsulphatase aarylsulphatase b activity ratio was all exp treatments produced a significant decrease in the percentage of lysosomal arylsulphatase present as the a form and an increase in that present as the b form and the activity ratio of arylsulphatase aarylsulphatase b declined the magnitude of these changes increased in the following direction starvation for hsham hepatectomy less than glucagon starvation less than subtotal hepatectomy these results indicate that the arylsulphatase aarylsulphatase b activity ratio in CL lysosomes of normal rats is maintained within rather narrow limits and this ratio declines during enhanced autophagocytosis these findings together with observations that suggest that arylsulphatase b may be a partially degraded form of arylsulphatase a are consistent with the view that the a form is more rapidly converted into the b form during autophagy owing to the digestive activity of the other lysosomal hydrolases present in autophagic vacuoles
|
MEDAL
|
ROS species ros are mediators of lung injury and glutathione gsh is the L1 nonprotein antioxidant that protects the cell from oxidative stress we have recently shown that ho induces ceramidemediated apoptosis in A549 cells we hypothesized that rosmediated SD of gsh plays a RII role in ceramide generation and thus in the induction of apoptosis our present studies demonstrate that gsh at physiologic concentrations to mm inhibits ceramide production in a time and dosedependent manner in a human alveolar epithelial cells on the other hand buthioninesulfoximinemediated depletion of intracellular gsh induces elevation of ceramide C2 and apoptosis in addition gsh blocks homediated induction of intracellular ceramide generation and apoptosis these effects were not mimicked by Ox gsh gssg or other thiol antioxidants such as dithiothreitol and mercaptoethanol moreover increase of intracellular ho mediated by inhibition of catalase by aminotriazole also induces ceramide generation and apoptosis these effects were blocked by nacetylcysteine our results suggest that gsh SD may be the link between oxidative AS and ceramidemediated apoptosis in the lung
|
MEDAL
|
we proposed a new RPA to treat chronic myelogenous one using a combination of busulfan and bestatin twentythree patients with ph positive cml including in the chronic phase and in the AP were treated with the ACT CHR was obtained in all patients CCR ccr was obtained in patients partial cytogenetic response pcr in and minor cytogenetic response mcr in in particular of patients in early chronic phase achieved ccr pcr mcr CR suppression of phclone was further confirmed by molecular analysis cytogenetic conversion to a normal diploid state persisted for months median months three year survival rate was confidence limit furthermore the longterm CHR in the accelerated phase patients without progression of the disease may be indicative of a LTS we conclude that bestatin effectively controls cml and allows reappearance of 2C hemopoietic cells in some patients the rationale for this ACT is that busulfan suppresses the proliferation of leukemic progenitors and decreases RT burden to the level where bestatin exhibits its antiproliferative effects bestatin is not merely antiproliferative but provides more complex biological properties capable of enhancing repopulation of normal residual stem cells
|
MEDAL
|
burning mouth syndrome bms is a chronic condition most common in middleaged and elderly women with prevalence rates in the GP ranging from to defined by the international headache society as an intraoral burning sensation for which no medical or dental cause can be found bms is considered a form of neuropathic pain the management of bms remains unsatisfactory in this pilot T0 we investigated the use of acupuncture in a small group of bms patients the study group T3 refusals was composed of bms patients females and male mean age years range from to years mean duration of bms years sd ± years oral painburning sensation primary outcome was measured using a VAS scale vas healthrelated SQ of life AA outcome was measured using the SF-36 health survey sf acupuncture treatment lasted weeks and consisted of sessions patients reported a mean reduction in pain of points on the vas max min which although slight was statistically significant wilcoxon test p no significant improvement in the overall score for quality of life was observed although subjects receiving AP treatment seemed better able cope with their oral symptoms
|
MEDAL
|
neuropeptides affect an extremely diverse set of physiological processes neuropeptides are often coreleased with neurotransmitters but unlike neurotransmitters the neuropeptide target cells may be distant from the CS of secretion thus it is often difficult to measure the amount of NPY PR in vivo by EP methods here we establish an in vivo system for studying the developmental expression processing transport and PR of neuropeptides a gfptagged atrial natriuretic factor fusion preproanfemd was expressed in the drosophila NS with the panneural promoter elav during embryonic development proanfemd was first seen to accumulate in synaptic regions of the cns in stage embryos by the third instar larval stage highly fluorescent SN were evident throughout the cns in the AD fluorescence was pronounced in the mushroom bodies AL and the CC at the larval neuromuscular junction proanfemd was concentrated in nerve terminals we compared the release of proanfemd from synaptic boutons of nmj which contain almost exclusively glutamatecontaining clear LDV to those of nmj which include the peptidergic type iii boutons upon depolarization approximately of the tagged NPY was released from nmjs of both muscles in min as assayed by decreased fluorescence although the elav promoter was equally active in the motor neurons that innervate both nmjs and nmj contained fold more neuropeptide and released much more proanfemd during stimulation than did nmj our results suggest that peptidergic SN have an enhanced ability to accumulate andor release neuropeptides as compared to neurons that primarily PR classical neurotransmitters
|
MEDAL
|
the urinary excretion of cyclic amp was studied during the first days of life in randomly selected infants admitted to a neonatal intensive care unit the data were compared with those of normal newborn infants urinary cyclic amp concentrations were significantly correlated with gestational age all patients and with birth weight all patients except infants of diabetic mothers idms the urinary cyclic ampcreatine ratio increased from day to day in normal newborns and in idms and tended to increase also in smallforgestational age sga low birth weight lbw and sick term infants although the changes in the latter CG were not statistically significant four infants studied with parallel determinations showed increased cyclic ampcreatinine ratio from day to day both in plasma and urine all urinary cyclic ampcreatine ratios were lower than the corresponding ratios found in plasma in lbw infants there was an inverse relationship between urinary cyclic amp and serum calcium in idms a positive rho was observed between urinary cyclic amp and blood glucose concentration in conclusion the excretion of cyclic amp in sick newborn infants is influenced by the following AF gestational age postnatal age birth weight and derangements of SS calcium and blood gl concentrations
|
MEDAL
|
the nonfimbrial adhesin nfa from uropathogenic escherichia coli okh mediates the agglutination of human red cells rbc notably of blood group mm the adhesin can be separated from the bacteria by heat extraction and was purified to homogeneity by AS precipitation and anion exchange chromatography in the presence of m urea nfa consists of noncovalently linked kda subunits which tend to form aggregates of an apparent molecular weight in excess of da the first aminoterminal amino acids were sequenced and no homology of this region was found with that of the blood group m TPS nonfimbrial adhesin of an unrelated uropathogenic e coli it has however an about homology to the corresponding region of the k antigen from animalpathogenic enterotoxic e coli both polyclonal and monoclonal antibodies against nfa were prepared one of the monoclonal antibodies strongly inhibits the hemagglutinating activity of both whole bacteria and purified nfa
|
MEDAL
|
modification to find the best physicochemical properties of minocycline hydrochloride ointment for optimum treatment of bedsore was investigated by coformulating various types of cyclodextrins cyd in the ointment base it was found that the drug PR rate from the ointment base was modified according to the preparation method of ointment base and the type of cyd admixed the physicochemical properties such as viscosity elution volume water absorption of ointment base were also modified by those factors the mechanism of physicochemical modification with cyd was explained by efficient methylacceptor in vitro than normal liver trna while endogenous trna isolated from the methylase fraction was a threefold more efficient methylacceptor than normal CL trna homologous methylation of normal trna as observed here has not been described before
|
MEDAL
|
fiftyseven adult AH and comparison patients without MLD were evaluated using a newly developed western blot assay for igm antibody to hepatitis e virus the mean age of patients with hepatitis was years range years were male among patients with acute hepatitis SH a antihav igm positive was diagnosed in two SH b antihbc igm positive in three and SH e antihev igm positive in one SH patient had cmv igm another had ebv igm and others were negative for all serologic markers of acute VH no patient with acute SH a or b and none of the comparison patients without acute SH had antihev igm all but one case of acute SH e were found among expatriates of asian origin and acute SH e was associated significantly with recent travel to the ISC these data suggest that acute SH e is common among foreign workers in kuwait but that little hev transmission is occurring directly in kuwait
|
MEDAL
|
the use of antilcell adhesion molecule monoclonal antibodies antilcammab in an endometriosis epithelial cell L1 z led to a statistically significant decrease in cell proliferation and cell invasion and to an inhibition of the adhesion compared with unspecific iggab treated and untreated cells because it increases the cell invasion and adhesion which consequently aggravates the disease l could possibly promote EM OD thus further studies should evaluate the possible use of antilmab in an animal EM model
|
MEDAL
|
okadaic acid a phosphatase inhibitor from a marine organism mimics tumor necrosis factorinterleukin tnfil in inducing changes in early cellular protein phosphorylation a total of approximately proteins exhibit significant and concordant changes in phosphorylation or dephosphorylation within min in human fibroblasts G1 by either okadaic acid tnf or il the fidelity of this mimicry by okadaic acid extends to the phosphorylation of the hsp complex stathmin eife myosin CS chain nucleolin epidermal growth factor receptor and other cdckinase substrates cabl rb and p the okadaic acidinduced DP of protein phosphorylation is distinct from that observed in cells treated with PMA acetate or with ligands like epidermal growth factor cAMP agonists bradykinin or interferons like tnf okadaic acid also induces the transcription of IE1 response genes like cjun and egr as well as the interleukin genes the overall early effects of okadaic acid uniquely parallel those of tnfil and not those of other cytokines or ligands regulation of protein phosphatase inhibition is discussed as a mechanism for tnfil ST
|
MEDAL
|
protectin cd is a glycolipidanchored inhibitor of the membrane attack complex mac of human complement c that protects blood cells EC cells and various epithelial cells from cmediated lysis because of its MICs protectin is a candidate molecule for use in the treatment of PNH or conditions where mac causes tissue damage soluble phospholipidfree forms of protectin have been isolated from human urine and produced in recombinant form but they have only a relatively weak c lysisinhibiting activity in the present study we have looked for functionally active protectin in human breast milk milk is rich in fat droplets milk fat globules mfg that are enveloped in a BPM derived from secretory cells of the MG the membranes of mfg contain a variety of glycoproteins expressed by the mammary epithelial cells both immunofluorescence and immunoblotting analysis demonstrated that protectin was strongly expressed on human mfg in sodium dodecyl sulphatepolyacrylamide gel PACE sdspage analysis mfg protectin cdm appeared as distinct bands with apparent molecular weights of mw similar to protectin extracted from mcf breast carcinoma cells cdm in BM was functionally active and had a glycophospholipid anchor as judged by its ability to incorporate into guineapig erythrocytes and inhibit their lysis by human complement these results indicate that functionally AS protectin becomes EC in mfg and imply that secretion of glycophospholipidanchored molecules eg into cow milk and colostrum could be exploited as a means of producing bioactive molecules that need to be targeted into cell CM
|
MEDAL
|
in an attempt to analyze structure CF and evolution of hiv gp v interactions among the HF SE the conformational entropy and the shannon entropy were determined for the nj set of antibodybound v loop conformers the HF energy of each conformer was determined at the mini level with gamess the conformational entropy was determined per conformer and per residue from the massweighted covariance matrices the shannon entropy per residue was determined from sequencesubstitution frequencies correlations were determined by LR analysis there was a negative rho between the HF SE and the conformational entropy r p df that enhanced the negative helmholtzfreeenergy NC for the binding of the gp ligand to target cd the shannon entropy of v was a function of the conformational entropy variance r p df and of the v hartreefock energy biological implications of this work are that conformational entropy interacts with v HF energy to enhance gp IB to cd cell receptors and that the HF energy of v interacts with the evolutionary system to participate in the regulation of v diversity
|
MEDAL
|
sepharose ionexchange particles bearing strong lewis acidbase PET groups sulfopropyl carboxymethyl quaternary ammonium dimethyl aminoethyl and iminodiacetic actromal transplantation assumed keratocyte phenotypes furthermore bm cells were isolated from keracrezeg mice a double TGM L1 in which cells expressing keratocan become egfp due to the synthesis of cre driven by keratocan promoter three days T3 corneal and conjunctival transplantations of such bm cells into kera mice green keratocan positive cells were found in the cornea but not in conjunctiva it is worthy to note that transplanted bm cells were rejected in weeks msc isolated from bm were used to examine if bm mesenchymal stem cells bmmsc could assume keratocyte phenotype when bmmsc were intrastromaltransplanted into kera mice they survived in the cornea without any immune and inflammatory responses and expressed keratocan in kera mice these observations suggest that corneal intrastromal transplantation of bmmsc may be an effective treatment regimen for corneal diseases involving dysfunction of keratocytes
|
MEDAL
|
the antiphospholipidantibody syndrome is associated with an increased incidence of arterial and venous thrombosis although renal infarction has been observed in these patients stenotic lesions of the renal i.a. associated with the antiphospholipidantibody syndrome have not been reported a yearold male with a history of hypertension for years developed blurred vision AA to thrombotic occlusion of the central retinal i.a. laboratory and radiologic examinations revealed RD a positive anticardiolipin antibody and narrowing of the right renal i.a. successful percutaneous transluminal renal i.a. angioplasty resulted in normalization of the blood pressure and REC of renal function
|
MEDAL
|
HS st cells a L1 derived from normal human gastric tissue HS TA a line derived from human gastric adenocarcinoma and hela cells were used together with hlabelled helicobacter pylori strain nctc to determine if cell surface glycosaminoglycans could act as initial receptors for adherence of the bacteria although as much as of the hlabelled bacteria adhered to monolayers of the cultured cells removal of glycosaminoglycans by prior treatment of the cells with heparitinase heparinase or chondroitin abc lyase had no effect in modifying the adherence prior addition of heparan sulfate heparin or chondroitindermatan sulfate to bacteria had no effect on adherence nor were bacteria released when these same glycosaminoglycans or these same enzymes were added to cultures already containing Ad bacteria these results indicated that neither heparan sulfate nor chondroitindermatan sulfate are involved as receptors in the initial adherence step of h pylori to these cultured cells
|
MEDAL
|
two hundred and thirty obese subjects age yr bmi kgm were studied before and after a week body mass reduction bmr program coupling restricted energy diet kcalday with low intensity exercise prescription it involved days per week consisting of onehour dynamic aerobic standing and floor exercise plus min of cycloergometer exercise at w or alternatively km outdoor leisure walking on flat terrain and psychological counseling oneleg standing balance test olsb and stair climbing test sct were employed to assess motor control and maximal lower limb muscle power respectively the bmr program induced a significant weight loss p a higher reduction of body mass index bmi being observed in males than in females p olsb performance time increased by p after treatment the improvement being evident in both genders a reduction in sct time p was also observed and corresponded to a increase p in average absolute muscle SP and increase p in TPS muscle power ie the power SO per kg of body mass with no differences between genders in conclusion in spite of the moderate reduction of body mass after restricted energy diet and low intensity physical conditioning significant improvements in motor control and performance likely to ameliorate the execution of simple ADL were observed in obese subjects
|
MEDAL
|
chemoradiation after surgery for locally AGC improves overall and relapsefree survival compared with observation however locoregional recurrences remain high accordingly we instituted this pilotfeasibility T0 including intraperitoneal fluorodeoxyuridine ip fudr as part of the treatment
|
MEDAL
|
the ultrastructure of the myocardium of the C1 gm fruiteating bat eidolon helvum provides further evidence that among rat species different types of cardiac myocytes are morphologically heterogeneous atrial cells of this species like those of smaller bats previously studied have few of the natriuretic hormonecontaining LG these granules are also small with a mean diameter of nm in comparison with those of the rat nm and mouse nm in all types of myocytes of the bat investigated mitochondrial matrix granules are more prominent than in those of the rat and mouse also distinctive are the purkinje cells which aside from their C1 size microns and lack of ttubules have atrial granulelike inclusions these cells are well endowed with mitochondria of cytoplasmic volume and with lipid bodies but have few glycogen LG their myofibrillae are well aligned the ventricular cells average diameter microns have nearly equal volumes of mitochondria and myofibrils mitochondrial cristae however are not compactly organized lipid material is found in the matrix of the mitochondria extensive evaginations of endothelial cells of the bat Endo appear continuous with EC vacuoles in these endothelial cells there are also many dense LG of varying shapes and sizes
|
MEDAL
|
to establish the most effective radiation dose for treatment of graves ophthalmopathy go
|
MEDAL
|
exudative retinal detachment as subretinal fluid accumulation occurs due to chorioretinal eye disease inflammation infection RT vascular abnormalities and in SVR diseases too the aim of this case report was to emphasize connection between the ophthalmological pathology and other SVR conditions this is a case report of a year old woman who suffered from pulmonary hypertension caused by many recurrent thromboembolic events during one of eight previous hospitalizations she complained of blurred vision BL exudative detachment is diagnosed by an ophthalmologist parenteral steroids and acetazolamide provided quick recovery
|
MEDAL
|
a murine homologue of the epithelial membrane protein emp gene was identified in a search for genes associated with bcell lymphoma tumorigenicity by using suppression subtractive hybridization expression of emp messenger rna in primary mouse tissues was limited to certain epithelial cell types and the peritoneal lymphoid compartment emp was expressed in the poorly tumorigenic dac blymphoma cell line but was significantly downregulated in a subline selected for in vivo RT formation in balbc mice recombinant restoration of emp expression in the subline suppressed its tumorigenicity suggesting that loss of emp was a causal factor in the malignant phenotype recombinant overexpression of emp was studied in b lymphoma and niht cells emp in both cell types induced cell death on serum deprivation empinduced cell death correlated with the expression level of emp protein and was prevented by caspase inhibitors zvad and zdevd these findings for the first time describe an apoptotic effect of a gas family gene in lymphocytes they also suggest that emp may influence blymphoma tumorigenicity through a functional RT suppressor phenotype blood
|
MEDAL
|
in this T0 our purpose was to compare the highresolution contrastmaterialenhanced threedimensional subtraction mr angiography with conventional angiography for occlusive disease in the pelvic and LE arteries a threedimensional fastimaging with steady precession fisp CS with a x matrix was obtained on t mr unit using a phased array coil twenty patients with arteriosclerotic obstructive disease underwent subtraction dynamic contrastenhanced mr angiography in patients three regions pelvis upper knee and lower knee were sequentially obtained after repeated i.t. of gadoliniumdiethylenetriamine pentaacetic acid gddtpa in the other five patients one region was imaged total of examinations a maximumintensity projection mip algorithm was used for subtracted images all patients also underwent conventional angiography angiographic images were divided into several anatomical segments three blinded radiologists independently graded a total of anatomic segments with stenotic or obstructive diseases and segments without disease subtracted images allowed resolution of small CB vessels in all examinations although misregistration was seen in eight examinations of five patients all arteries larger than mm in diameter were visualized on subtracted images for PCD of significant stenosis mr angiography had sensitivity and specificity the rho coefficient of degree of agreement between mr angiography and CA was stenotic vessels tended to be overestimated we conclude that highresolution DCE threedimensional mr angiography is capable of depicting small vessel anatomy of the pelvis and lower extremities sequential mr angiography of different regions was feasible by repeated i.t. of gddtpa and subtraction this technique is highly sensitive in detecting lesions but stenosis tended to be overestimated
|
MEDAL
|
gene therapy is a rapidly developing field in which recombinant nucleic acid sequences are introduced to individuals to regulate repair replace add or delete a genetic sequence recombinant adenoassociated viral aav vectors especially aav are frequently used in gene therapy knowledge on the biodistribution and potential shedding of aav is crucial to evaluate the risks of infection with the viral vector for the patient and the environment literature was analysed for biodistribution and shedding data for aav preclinical and clinical studies were included with a focus on the influence of the administration route on spreading based on biodistribution and shedding data a qualitative model for the biodistribution and shedding of aav related to the administration route is presented it is concluded that biodistribution and shedding of aav depends on the route of administration some routes lead to local biodistribution and thus to no shedding or shedding via one route only other routes lead to systemic biodistribution and to shedding via several excretion routes the qualitative model presented can help to determine the possible biodistribution in the body and the risk of shedding via the different excretion routes in addition it can help to predict the different shedding routes after a certain administration route of aav and thus in deciding which studies are warranted or which safety precautions are needed after administration to patients
|
MEDAL
|
Subsets and Splits
No community queries yet
The top public SQL queries from the community will appear here once available.