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the HR muscle responds to physiological needs with a ST modulation of cardiac contractility this process is determined mainly by properties of the cardiac ltype ca channel cav including facilitation and cadependent inactivation cdi both facilitation and cdi involve the interaction of calmodulin with the iq motif of the cav channel especially with ile to verify this hypothesis we created a mouse L1 in which ile was mutated to glu cavie mice homozygous cavie mice were not viable therefore we inactivated the floxed cav gene of heterozygous cavie mice by the αmyosin heavy chainmercremer system the resulting ie mice were studied at day after treatment with tamoxifen EP recordings in ventricular cardiomyocytes revealed a reduced cav current ica density in ie mice steadystate inactivation and REC from inactivation were modified in ie versus control mice in addition voltagedependent facilitation was almost abolished in ie mice the time course of ica inactivation in ie mice was not influenced by the use of ba as a charge carrier using bisoaminophenoxyethanennnntetraacetic acid as a chelating agent for intracellular ca inactivation of ica was slowed down in control but not ie mice the results show that the ie mutation abolishes cacalmodulindependent regulation of cav the cavie mutation transforms the channel to a phenotype mimicking cdi | MEDAL |
saliva from subjects with amebic ALA ala acute amebic colitis asymptomatic infection with Eh or entamoeba dispar and uninfected controls was tested by enzymelinked immunosorbent assay elisa for the presence of e histolytica galactoseinhibitable lectin antigen and salivary immunoglobulin igg antibodies to a recombinant cysteinerich lectinderived protein lc salivary lectin antigen was found in of subjects with acute colitis compared to of those convalescent from ala with asymptomatic e histolytica infection with e dispar infection and of controls subjects from the united states and T0 patients with nonamebic diarrhea p for each compared to values for subjects with colitis salivary antilc igg antibodies were found in of ala patients regardless of duration of illness and in of colitis patients who were symptomatic for at least days p compared to other T0 CG SS antilc igg antibodies were detected in of subjects with acute colitis of subjects with ala or prolonged colitis of subjects with asymptomatic e histolytica infection of subjects with e dispar infection and of diarrhea controls in comparison to elisa for SS antilc igg antibodies the salivary lectin antigen CA is a more CS and specific test for acute amebic colitis detection of salivary antilc EA by elisa is an effective means for the diagnosis of ala and prolonged cases of amebic colitis | MEDAL |
cell numbers have been investigated for several months in disinfectant solutions which were measured out with tap water by decentral automatically dosing apparatuses they differed not only quantitatively but also qualitatively with respect to the products used with one product on the bases of aldehydes organic tin compound h after samples have been collected up to bacteria ml could be cultivated thus cell numbers in disinfectant solutions were in the same range of cell numbers of TW sometimes the cell numbers in both substrates seem to go parallel fig the bacterial compositions in both were similar table gramnegative yellow bacteria of the genera pseudomonas and flavobacterium dominated after the disinfectant in one apparatus has been substituted by one which is composed of aldehydes a quaternary ammonium compound cell numbers rapidly and permanently decreased to the limit of detectability only coryneform bacteria survived for a longer time which were characterized tab but not determined taxonomically their facultatively methylotrophic growth was conspicuously | MEDAL |
in china flue gases emitted by coalfired SP plants are mainly cleaned using electrostatic precipitators esps however based on observations there is a decrease in the CE of esps in some power plants after burning jungar coal in inner mongolia in order to find the mechanism of CFA escaping from esps the size CSD resistivity and cohesive force of particulate matter samples from jungar coalfired SP plants in china were measured using a bahco centrifuge a dust electrical resistivity test instrument and a cohesive force test apparatus invented by the authors experiments were carried out to determine the chemical composition and currentvoltage curve of fly ash under operating esps the alo content in fly ash was found to reach more than with the size CSD showing a higher content of pm and pm in highalumina coal fly ash than in other coal fly ashes the resistivity of highalumina CFA was recorded at over Ï cm but this did not result in a clear back corona the cohesive force of highalumina coal FA was very little it was CS to smoke speed in the electric field facilitating dust reentrainment | MEDAL |
MEN type is historically composed of three clinical subtypes all of which are associated with germline mutations in the ret protooncogene multiple endocrine neoplasia type a familial MCT and multiple endocrine neoplasia type b are collectively associated with a risk of medullary thyroid carcinoma by age years pheochromocytomas are identified in of individuals with MEN type a and MEN type b furthermore those with MEN type a have a risk for primary hyperparathyroidism individuals with multiple endocrine neoplasia type b often have distinct PCS features including mucosal neuromas of the lips and tongue medullated corneal nerve fibers ganglioneuromatosis of the gastrointestinal tract distinctive facies with enlarged lips and a marfanoid body habitus clinical recognition and accurate diagnosis of individuals and families who are at risk of harboring a germline ret mutation is critical for the prevention and management of potentially lifethreatening neoplasms this overview summarizes the clinical description of multiple endocrine neoplasia type diagnosis and testing strategies management and surveillance and differential diagnosis for other related syndromes | MEDAL |
brief changes in the cytosolic and intraorganellar ca concentration serve as specific signals for various physiological processes in mesothelial cells lining the surface of internal organs and the walls of body cavities a reentry in the Tc gg transition evoked by serum readministration induces longlasting ca oscillations with a slowly decreasing frequency individual mesothelial cells show a wide range of different oscillatory patterns within a single supposedly homogenous cell population changes in the cytoplasmic ca concentration ccyt show baseline oscillatory patterns ie discrete ca transients starting from a constant basal ccyt level the er ca concentration cer displays a sawtooth wave at a semidepleted er state the minimum level is reached just briefly after the maximal value for ccyt these oscillations depend on plasmalemmal ca influx and on the Ins trisphosphate concentration insp the ca influx is a crucial determinant of the oscillation frequency partial blocking of serca pumps modifies the oscillation frequency in both directions ie increasing it in some cells and lowering it in others current mathematical models for ca oscillations mostly fail to reproduce two experimentally observed phenomena the broad range of ISI and constant basal ccyt C2 between two ca spikes here we developed a new MM based onand fitted toca recordings of ccyt and cer recorded in primary mouse mesothelial cells the model allowed for explaining many features of experimentally observed ca oscillations we consider this model to be suitable to simulate various types of insp receptorbased baseline ca oscillations | MEDAL |
inflammatory pseudotumor of the CL which is rarely seen can mimic abscesses metastases IP cholangiocarcinomas and HCC carcinomas while it has no specific clinical and laboratory findings detection of a targetlike appearance in triphasic ct and mri examination may be differentiated from other CL pathologies in this report triphasic ct and mri findings of an inflammatory pseudotumor of the liver were presented | MEDAL |
a yearold woman presenting with thrombocytepenia and signs of intravascular coagulation prothronbin time fdp microgramsml due to a giant hemangioma of the liver kasabachmerritt syndrome was scheduled for the resection of the RA lobe of the CL in order to protect the liver on occlusion of the right hepatic i.a. and portal vein we induced mild HT technique with vasodilation and surface cooling by convecting warming device together with hepatoprotective agents of pge and ulinastatin severe acute massive bleeding occurred due to the injury of the middle hepatic vein and from the resected surface of the liver her rectal temperature was degrees c during massive one her hemoglobin decreased to gdl total estimated blood loss was ml the weight of the resected CL was kg the maximum postoperative levels of tbil and got were mgdl and iul respectively the liver seemed to have been well preserved and no other complications were observed the hemangioma was completely removed by excision of the RA lobe of the liver subsequently all coagulation parameters returned to normal indicating a CR reversibility of the coagulopathy surfaceinduced mild HT is a useful and valuable method for protecting the liver during severe massive one | MEDAL |
a yearold male with no PH of head trauma suffered CSF fluid csf discharge from the left nostril for one month coronal computed tomography ct showed lateral extension of the sphenoid sinus on both sides and csf collection on the left side ct cisternography could not identify the site of csf leakage heavily tweighted magnetic resonance mr imaging mr cisternography in the coronal plane clearly delineated a fistulous tract through the sphenoid bone into the sphenoid sinus patch VG with muscle fragment completely relieved the csf rhinorrhea postoperative threedimensional ct showed the two bone defects identified during surgery small bony dehiscences in the sphenoid bone and lateral extension of the sphenoid sinus predisposed the present patient to csf fistula formation mr cisternography in the coronal and sagittal planes is superior to ct scanning or CTC for detection of the site of AS csf leakage | MEDAL |
the ligand binding domain of g proteincoupled receptors for peptide ligands consists of a pocket formed by extracellular and TM domain tm residues in the case of somatostatin srif however previous studies have suggested that the IB cavity of the octapeptide analog sms sms is lined by residues in tms iiivii the additional involvement of the extracellular domains for IB sms or the NK srif ligands srif srif has not been clarified using a cassette construct cdna for the human somatostatin receptor sstr we systematically examined the role of exofacial structures in ligand IB by creating a series of mutants in which the extracellular portions have been altered by conservative segment exchange cse mutagenesis for the extracellular loops ecls and by deletion for the nhterminal segment or truncation analysis ecl chok cells were stably transfected with wild type or mutant human sstr constructs and agonist binding was assessed using membrane binding assays with iltt srif ligand deletion of the nh terminus or cse mutagenesis of ecl and ecl produced minor fold decreases in affinity for srif srif and sms ligands truncation of ecl to mimic the size of this loop in sstr and sstr the two subtypes that do not bind sms did not interfere with the IB of sms srif or srif in contrast both ecl mutants failed to bind iltt srif immunocytochemical analysis of nonpermeabilized cells with a human sstr antibody revealed that the mutant receptors were targeted to the plasma membrane labeled sms ityr sms also failed to bind to the mutant ecl receptors these results suggest a potential contribution of ecl in addition to the previously identified residues in tms iiivii to the srif ligand IB pocket | MEDAL |
the expression of the Bcl-2 molecules bcl and transforming growth factorbeta is known to confer protective effects on the CBF ischemiareperfusion injury the current study investigated the expression levels of bcl and transforming growth factorbeta in response to multiple preischemia EAP at acupoints zusanli st and fengchi gb stimulation rats were divided into five groups uninjured control nonacupoint gb and st rats in the nonacupoint gb and st CG received T2 times or times of electroacupuncture stimulation before exp CBF ischemia was induced bcl and transforming growth factorbeta were found to be significantly increased in the st groups with either or electroacupuncture treatments p the production was higher with EAP treatments in the st groups p in the gb CG significant increase was only observed in transforming growth factorbeta with EAP treatments p no significant elevation of the level of transforming growth factorbeta was observed in the nonacupoint CG however the production of bcl increased with treatments in the nonacupoint CG p the data suggest that multiple preischemia electroacupuncture at st was effective in conferring neuroprotective effect on the brain by means of upregulation of bcl and transforming growth factorbeta and the effect was increase with the number of treatment | MEDAL |
the vo vco rq blood flow and developed tension of in situ dog gastrocnemius muscle were studied during contractions at twsec for both normoxic and hyperoxic PLB conditions vo and DT were not significantly altered by the breathing gas changes cao rose by during obreathing however a significant decrease of in muscle blood flow resulted in no significant change in otransport cao x q to muscle comparing hyperoxia to normoxia hyperoxia resulted in a significant decrease in muscle vco and rq the cause of the rq NC with PLB gas o content is not certain however evidence is presented indicating that infused EN and propranolol can effect the response | MEDAL |
novel proteins synthesize predominantly in bone have been identified by antibody screening of bone cell cdna expression libraries two unique cdnas were identified whose structures do not match any known nucleic acid or protein CS in the nih computer bank the first cdna clone bpi encoded a mrna of bases in size which was expressed at high levels in rat osteosarcoma cells rat calvarial bone cells and placenta a second clone bpii encoded a mrna of bases which was expressed at high C2 in osteosarcoma cells and in salivary gland expression of both mrnas in OSA cells was modulated by the calciotropic hormone vitamin d SB analyses indicated that the two cdnas represented distinct single copy genes in the rat genome these novel gene products may serve as potential new markers to T0 bone turnover in metabolic bone disease | MEDAL |
a yearold men with RA ICA of year history presented with severe chest pain the electrocardiogram was consistent with acute inferior myocardial infarction TTE showed increased left ventricular dimensions and HK in the inferolateral wall coronary angiography performed for percutaneous coronary intervention showed aneurysmatic dilatation mm and total occlusion of the right coronary artery by a C1 thrombus as there was no stent available for dilated right PD and due to the C1 thrombus burden medical therapy was decided and tissue plasminogen activator infusion was started the patients CP progressively decreased coronary angiography performed on the fifth day of admission showed timi flow in the right coronary i.a. warfarin was added to standard antiischemic treatment with a target inr of our literature search yielded no reported case of such aneurysmatic dilatation associated with RA arthritis | MEDAL |
from the xray coordinates of bovine trypsin and its complexes with substrate analogues benzamidine and with soyabean TR inhibitor a peptide tp was designed and synthesized by surfacesimulation synthesis a concept previously introduced by this laboratory to mimic the IB site of trypsin also a control peptide ctp was synthesized that contained all the amino acids present in the tp peptide except that their order was randomized the radioiodinated tp peptide bound specifically to adsorbents of benzamidine whereas the control ctp peptide exhibited no IB activity conjugates to succinyl carboxypropionyllysozyme of the tp peptide control ctp peptide and other unrelated MMP were examined by a radiometricmic patients fpg mgdl tg mgdl and five nondiabetic hypertriglyceridemic patients fpg mgdl tg mgdl IB of ilabeled ldl from HTG subjects with and without diabetes to cultured skin fibroblasts was significantly decreased to and of that seen with ldl from NL nondiabetic subjects and diabetic normolipidemic controls p less than unlabeled ldl from HTG subjects with and without diabetes failed to suppress ldl receptor activity and sterol synthesis from cacetate as efficiently as unlabeled ldl from healthy subjects the ability of ldl from hypertriglyceridemic subjects whether diabetic or not to suppress ldl binding was inversely related to the ratio of triglyceride to protein in ldl r p less than and showed a positive rho with the ldl cholesterolprotein ratio p less than thus ldl from patients with hypertriglyceridemia with or without coexistent diabetes shows impaired binding to ldl receptors and less ability to downregulate ldl receptor activity and sterol synthesis than does ldl from normolipidemic diabetic and nondiabetic subjects these findings suggest that factors associated with hypertriglyceridemia rather than with diabetes result in altered metabolism of ldl in these disorders | MEDAL |
to examine the serum levels of dehydroepiandrosterone sulfate dheas and its relation with insulin resistance and the other risk AF in essential hypertension SS dheas and insulin sensitivity were assessed in male hypertensive and male healthy control subjects aged years fasting plasma insulin and the area under curve of PI were determined during a g oral gl ATT insulin sensitivity was measured by the Css PG method FPI and the area under curve of PI were significantly higher in the hypertensive group than in control group steady state PG was significantly higher in HT indicating insulin resistance compared with control subjects on the other hand fasting serum dheas levels were significantly lower in the hypertensive group than in the control group fasting serum dheas C2 were inversely correlated with Css PG significantly p indicating a close association between dheas levels and insulin resistance fasting serum dheas was inversely correlated with systolic blood pressure and FPI in MRA of hypertensive subjects steady state plasma glucose was the strongest determinant of the fasting serum level of dheas followed by systolic blood pressure and fasting plasma insulin these AF accounted for of the variation in dheas in nonobese and nondiabetic essential hypertension SS dheas was lower and insulin resistance was the most significant independent determinant of reduced SS dheas followed by systolic blood pressure and FPI | MEDAL |
the effects of SM of blood pressure on the control of hypertension were examined in this T0 failure of patients to comply with treatment is presumably attributable in part to the fact that hypertension usually is asymptomatic until complications develop selfmonitoring might make visible an otherwise asymptomatic condition and thereby increase motivation one hundred hypertensive patients beginning OP treatment were randomly assigned to experimental and control CG subjects were given a sphygmomanometer and instructed in its use both groups were given similar antihypertensive medications after six months of treatment mean SP was significantly lower mm hg p smaller than in the exp than in the control subjects however the mean baseline systolic pressure in the control group was mm hg less than that of the exp group if this value is substracted from the difference between the last mean systolic pressures in the two groups the mm hg difference is seen as a very modest effect of selfmonitoring diastolic blood pressure was insignificantly lower for exp subjects compared to the potent effectiveness of drugs in reducing blood pressure SM was of little value | MEDAL |
reticuloendotheliosis virus rev is an important representative avian retrovirus to improve our understanding of the host cellular responses to virus infection and the pathogenesis of rev infection we applied isobaric tags for relative and absolute quantification itraq labeling coupled with multidimensional liquid chromatographytandem mass spectrometry to detect changes in protein C2 in CE fibroblast cells cefs that were INF with rev or mock infected in total cellular proteins were differentially expressed among which and were differentially expressed in revinfected cefs at and days postinfection respectively gene ontology analysis indicated that the biological processes of the differentially expressed proteins were primarily related to cellular processes metabolic processes biological regulation response to CS- and immune system processes and that the molecular functions in which the differentially expressed proteins were mainly involved were binding catalytic activity and enzyme regulator activity pathway analysis showed that a total of and pathways including protein digestion and absorption focal adhesion ecmreceptor interaction cytokinecytokine receptor interaction tolllike receptors and jakstat signaling were EC in revinfected cefs at and days postinfection respectively in conclusion this study is the first to analyze the protein profile of revinfected cefs using an itraq approach the results of this study provide valuable information for better understanding the host response to rev infection | MEDAL |
in vitro cultivated atlantic salmon salmo salar l hepatocytes were incubated without or with a mixture of sesamin and episesamin in order to test for possible effects on lipid metabolism sesaminepisesamin SE mm final concentration led to increased elongation and desaturation of c n to docosahexaenoic acid c n dha p and down regulated gene expression of delta and delta desaturases compared to control treatment sesaminepisesamin further increased the hepatocytes capacity for fatty acid betaoxidation of c n p to the c acid soluble products acetate malate and oxaloacetate in agreement with an increased gene expression of carnitine palmitoyltransferase i also the mRNA of cluster of differentiation was upregulated and the expression of scavenger receptor type b peroxisome proliferatoractivated receptors alpha and gamma were downregulated the amount of triacylglycerols secreted by the cells tended to be lower in the sesaminepisesamin incubated hepatocytes than the control cells this study shows that sesamin has favourable effects on lipid metabolism leading to increased level of dha which may be of interest for aquaculture use | MEDAL |
the properties of CO and triolein in mixed monolayers at the airwater interface have been measured between and degrees c analysis of forcearea curves obtained as a CF of the mol fraction of CO indicates that at relatively low surface pressures these compounds are miscible in two dimensions up to a limit of about mol fraction at higher pressures either cholesteryl oleate or both lipids are expelled from the ML to form a bulk phase which is in rapid equilibrium with the surface phase in the ML phase orientation of the ester function of cholesteryl oleate is toward the aqueous phase interaction with TO is minimal and packing is uniform over the solubility range this together with the susceptibility of the CO to enzymatic hydrolysis suggests the applicability of ML systems to the study of cholesterol esterase activity comparison of our results with the bulk properties of these lipids suggests that the expelled CO exists as a smectic mesophase and thus the system may provide a model for studying the transfer of molecules between the interior and surface of lipid deposits of the type found in AS lesions | MEDAL |
proton nmr spectroscopy was applied to quantitate the measurement of total body waterfat distributions in vivo a special mr protocol was developed to excite a thick slab of tissue and display the magnitude nmr spectrum of the collected RS very short echo time te ms and long TR time tr ms were used to minimize EC50 damping of the signal intensities the spectrum was then decomposed into individual lines and proton densities of different species were calculated proton density information was converted into weight percentage data using stoichiometrical and physiological information the technique was validated using phantoms that contained different mixtures of watermaize oil a highresolution nmr spectrum of maize oil samples was used to determine the stoichiometric information the test results showed good agreement with the known composition of the phantom within the whole range of water content this method is very fast since no phase encoding of data is required preliminary results for monkeys show promising potential in clinical applications | MEDAL |
we report on the largescale synthesis of agnanoparticle agnp decorated znomesoporousnanosheets nss grafted on a flexible carbon fiber cloth cfc as CS and reproducible surface enhanced raman scattering sers substrates with excellent flexibility the composite serssubstrates are achieved by a combination of atomic layer deposition of znoseeds on each fiber of the cfc denoted as znoseedscfc chemical bath deposition and subsequent pyrolysis for the creation of znomesoporousnss grafted on znoseedscfc and ionsputtering of agnps on the znomesoporousnss as abundant sers hot spots are generated from the electromagnetic coupling of the densely distributed agnps and the semiconducting znomesoporousnss also have chemical supporting enhancement and distinct molecule adsorbing abilities the composite serssubstrates demonstrate high serssensitivity with good signal reproducibility as a trial for potential applications the composite serssubstrates were used to identify pesticides and highly toxic polychlorinated biphenyls pcbs and low concentrations down to m for methyl parathion and à m for pcb were reached respectively showing promising potential for the sersbased rapid PCD of toxic organic pollutants in the environment | MEDAL |
a diagnosis of crohns disease cd and ulcerative colitis uc is based on a combination of clinical histologic endoscopic and radiologic data the distinction between uc and cd can be difficult because of the lack of a differentiating single gold standard IC ic was introduced by pathologists for the diagnosis of surgical colectomy specimens showing an overlap between the features of uc and cd the diagnosis of ic was based on macroscopic and microscopic features the term indeterminate colitis is in recent years more widely applied to include all cases with endoscopic radiographic and histologic evidence of chronic inflammatory bowel disease confined to the colon but without fulfilment of diagnostic criteria for uc and cd as for uc and cd the diagnosis of ic has therefore become a clinicopathologic diagnosis ic is generally considered to be a temporary diagnosis the clinical characteristics of patients with ic are however somewhat different from the characteristics of those with uc furthermore serologic markers such as perinuclear ANCA and antisaccharomyces cerevisiae which are strongly linked with uc and cd are both negative in a subset of patients with ic therefore the possibility that ic could be a separate entity must be investigated | MEDAL |
it was reported recently that SE to an adverse rearing environment lowers CNS system cns serotonergic activity in a nonhuman primate rhesus monkeys but only among animals having the shorter variant of a functional biallelic repeat polymorphism in the RII region of the serotonin transporter htt gene because repeat variants of the same core sequence affect transcriptional efficiency of the htt gene in humans we examined whether biallelic R2 in the htt genelinked polymorphic region httlpr acts analogously to modulate a previously described association between SES status ses and cns serotonergic CF the httlpr was genotyped in AD men and women n and who were administered a standard NE challenge to assess central serotonergic responsivity plasma prolactin prl response to the serotonin releasing agent fenfluramine SES was estimated from reported income and years of education hierarchical LR showed serotonergic responsivity to be predicted by the interaction of httlpr genotype and ses p individuals of lower income and less education had lower peak prl concentrations following administration of fenfluramine than did subjects ranking higher on these dimensions but only among persons possessing at least one httlpr short S2 within genotype ses covaried moderately with the prl response to fenfluramine among subjects who were homozygous for the short allele r p a similar association was present at lesser magnitude in heterozygotes r p and absent among subjects homozygous for the long S2 r ns findings were comparable for men and women and persisted on reanalysis restricted to persons without current axis i psychopathology we conclude that allelic R2 at httlpr moderates the influence of social position on cns serotonergic responsivity | MEDAL |
the effect of energetic metabolism compromise obtained by chemical induction of hypoglycaemia GD hypoxia MRC inhibition and ischaemia hypoglycaemia plus hypoxia on glutamate toxicity was analyzed on pc cells the respiratory status of these cells measured by the mtt dimethylthiazolyldiphenyltetrazolium bromide CA was significantly decreased T3 metabolic inhibition induced by ischaemia but it was not affected by both hypoglycaemia and hypoxia under hypoglycaemia but not under hypoxia atp levels were significantly reduced from to nmolmg protein however ischaemiclike conditions greatly potentiated the decline of atp C2 decrease observed T3 hypoglycaemia the influence of metabolic inhibition on glutamateinduced cytotoxicity was also analyzed when the cells were preincubated under conditions that deplete atp hypoglycaemia and ischaemia the inhibition of mtt reduction measured after glu incubation was potentiated this effect could be reverted when vitamin e and idebenone were present during the induction of metabolic inhibition the atp C2 above which glutamate susceptibility was enhanced were also determined these results indicate that glutamate toxicity on pc cells which occurs by a mechanism independent of nmethyldaspartate nmda receptor activation can be enhanced by the SD of intracellular atp upon metabolic AS it is dependent on the extent of atp SD and seems to involve the generation of free radicals it can be concluded that under ischaemic conditions the deleterious effects of glutamate can be potentiated by the energetic compromise associated with this pathologic situation | MEDAL |
social desirability and intellectual limitations were investigated as possible determinants of two response biasesacquiescence and naysayingamong mentally retarded respondents results clearly indicated that naysaying and acquiescence occur more frequently among lower iq individuals and naysaying occurs more frequently concerning prohibited SMB whereas respondents acquiesce regarding desirable SMB these results suggest that intellectual limitations predispose people to biased responding whereas social desirability AF determine the type of bias | MEDAL |
nuclear magnetic resonance microscopy was used to image the parasitoid wasp venturia canescens hymenoptera ichneumonidae within larval and pupal instars of its host the indian meal moth plodia interpunctella lepidoptera pyralidae the images were obtained using gradientecho and CS selective PP sequences and clearly showed the location and shapes of the parasitoid as it developed from the l L3 to a pupal stage within the host the digestive nervous and tracheal systems of the host were identified and changes were observed as the host underwent metamorphosis destruction of the host tissues by the parasitoid was visible it was found that the parasitoid first ate the fat body and digestive system of the host allowing the host to continue to grow and only PD to the vital organs when its own OD had neared pupation | MEDAL |
the purpose of this T0 was to evaluate the tau and safety of tacrolimus eye drops in the tarsal form of vernal keratoconjunctivitis vkc and to survey the changes of dendritic cells at the palpebral conjunctiva in patients with vkc by in vivo confocal microscopy | MEDAL |
ten strains of enterotoxigenic escherichia coli producing HL enterotoxin lt were preserved under different conditions after month months and years of preservation the cultures were recovered and examined for lt production preservation of the cultures on dorset egg medium at degrees c and preservation by freezing the cell suspensions in tryptic soy broth with glycerol were found to be suitable preservation methods all strains were alive for years and had a minimum loss of lt production | MEDAL |
to determine the proportion of cats entering a florida animal shelter with serum antibody titers against feline panleukopenia virus fpv feline herpesvirus fhv and feline calicivirus fcv and to identify factors associated with seropositivity | MEDAL |
maturity onset diabetes of the young subtype mody is associated with defective glucosedependent insulin secretion from pancreatic beta cells mody is caused by mutation in the transcription factor hepatocyte nuclear factor alpha hnf alpha to understand better the mody phenotype we tested whether hnf alpha was able to modulate directly the insulin gene promoter transfection of cultured t cells with an hnf alpha expression vector led to fold activation of a cotransfected reporter plasmid containing the rat insulin i gene promoter computer analysis revealed a potential hnf alphabinding site between nucleotides and of the promoter mutation of this CS led to reduced ability of hnf alpha to activate the promoter the ability of hnf alpha to bind this CS was confirmed using gel shift analysis in transfected ins beta cells mutation of either the hnf alpha site or the hnf alpha site in the INS promoter led to reduction in reporter gene activity expression of dominant negative hnf alpha led to significant reduction in the activity of wild type and both mutated promoters thus in addition to the previously described indirect action of hnf alpha on insulin gene expression mediated through elevated hnf alpha C2 hnf alpha also activates the INS directly through a previously unrecognized cis PE | MEDAL |
in a dairy farm in lower saxony a male black and WG german holstein calf showed two partially fused heads scoliosis of the thoracal vertebral column and a highgraded dyspnoea the animal was sired by a bull used in artificial insemination further affected calves were not known at this farm the radiographic examination of the animal verified the diagnosis of a dicephalus the pathologicalanatomical examination revealed a complex malformation of the HR similar to the human eisenmengercomplex several environmental causes like intoxication rectal palpation for gestation diagnosis infections by bovine virus diarrhoea or infectious bovine rhinotracheitis viruses could be ruled out by anamnesis and virological examinations thus we supposed a hereditary background for this defect | MEDAL |
the enzyme hydroxyphenylacetate nadphoxygen oxidoreductase hydroxylating ec hydroxyphenylacetate monooxygenase referred to here as hpa hydroxylase was induced in pseudomonas acidovorans when hydroxyphenylacetate pha was utilized as carbon source for growth homogentisate and maleylacetoacetate were intermediates in the degradation of hpa a preparation of the hydroxylase that was free from homogentisate dioxygenase and could be stored at c in the presence of dithioerythritol with little loss of activity was obtained by ultracentrifuging cell extracts but when purified fold by affinity chromatography the enzyme became unstable FAD and mg ions were required for full activity hpa hydrocylase was inhibited by kcl which was uncompetitive with hpa values of ki determined for inhibitors competitive with hpa were mum dlhydroxymandelic acid mum dihydroxyphenylacetic acid mum hydroxymethylphenylacetic acid and mum hydroxyphenylpropionic acid apparent km values for substrates of hpa hydroxylase were mum hpa mum oxygen mum reduced nicotinamide adenine dinucleotide nadh and mum NADH phosphate nadph the same maximum velocity was given by nadh and nadph a chemical synthesis is described for deuterohydroxyphenylacetic acid this compound was enzymatically hydroxylated with retention of half the deuterium in the homogentisic acid formed activity as substrate or inhibitor of hpa hydroxylase was shown only by those analogues of hpa that possessed a hydroxyl group substituent at c of the benze nucleus a mechanism is suggested that accounts for this structural requirement and also for the observation that when hydroxyphenoxyacetic acid was attacked by the enzyme hydroquinone was formed by PR of the side chain probably as glycolic acid only one enantiometer of racemic hydroxyhydratropic acid was attacked by hpa hydroxylase the product alphamethylhomogentisic acid dihydroxyphenylpropionic acid exhibited optical activity this observation suggests that during its shift from c to c of the nucleus the side chain of the substrate remains bound to a site on the enzyme while a conformational change of the protein permits the necessary movement of the benzene ring | MEDAL |
the aim of this T0 was to investigate the activity and safety of a regimen containing carboplatin and paclitaxel in patients affected by recurrent or metastatic HNC eligible patients were treated with a week combination of paclitaxel mgm and carboplatin area under the concentration time curve mgmlmin for a maximum of four cycles a total of patients entered the study one patient had a CR response whereas six patients obtained a partial response SD was observed in seven patients the disease control rate was confidence interval whereas overall response rate was confidence interval the median overall survival was months range with a year survival of the median progressionfree survival was month range treatmentrelated deaths or episodes of neutropenic fever were not registered grades neutropenia was observed in two patients grades anaemia and TP in four and one patients respectively nine patients experienced grades and one patient grade IP neuropathy the combination of carboplatin and paclitaxel is safe and moderately effective for the treatment of recurrent or metastatic head and neck CA | MEDAL |
the personal health information management phim practices and needs of older adults are poorly understood we describe initial results from the uw soaring project studying older adults researching information needs and goals a participatory design investigation of phim in older adults years and older we conducted indepth interviews with older adults n living in a variety of residential settings about their management of personal health information a surprising of participants report usint offered advantage in terms of the load of the retentate and decrease in the endocrinedisrupting activity of the samples moreover the photolysis byproducts produced with higher endocrine disrupting activity than the parent EDC were effectively retained by the membrane the combination of direct lpuv photolysis with nanofiltration is promising for a drinking water utility that needs to cope with sudden punctual discharges or deterioration of the water quality and wants to decrease the levels of chemicals in the nanofiltration retentate | MEDAL |
family characteristics of high expressed emotion ee have been found to be predictive of relapse in schizophrenia and other psychiatric disorders a causal relationship has been postulated between relapse and components of ee as assessed in the relatives with whom the patient has close social contact recently conceptual and methodological aspects of the ee paradigm have become the focus of controversy we present the dilemmas under debate and discuss the implications relevant to the study of ee in israel | MEDAL |
we have previously demonstrated that granulocytemacrophage colonystimulating factor gmcsf receptors are composed of at least two molecules of and kda which were denoted alpha and betachains respectively chiba s shibuya k piao yf tojo a sasaki n matsuki s miyagawa k miyazono k takaku f cell regul in this paper we describe an investigation of the PSA disparity noted between the alpha and betachains of gmcsf receptors using proteolytic and deglycosidic enzymes and further demonstrate the potential importance of carbohydrate structures of the gmcsf receptors using different lectins and glycoprotein synthesis inhibitors crosslinked alpha and betachains with igmcsf were digested by staphylococcus aureus v protease and gave a different pattern furthermore the size of the alphachain was reduced by kda by the removal of the nlinked OS with peptidase nglycosidase f treatment whereas that of the betachain remained unmodified by the enzyme these results suggest that the alphachain of gmcsf receptors agrees with the recently cloned lowaffinity GMR gearing dp king ja gough n m nicola na embo j having approximately nlinked OS and is biochemically different from the alpha betachain by analyses using lectins some of the oligosaccharides in the alphachain seem to be the complextype andor hybridtype because wheat germ agglutinin and leukoagglutinating phytohemagglutinin inhibited both gmcsfinduced proliferation and gmcsf IB to its receptors further analyses using glycoprotein synthesis inhibitors showed that nlinked processing of the alphachain especially glucose removal by glucosidase i and ii whose MICs are inhibited by deoxynojirimycin appeared to be required for the expression onto the cell surface although the betachain expression was little affected by their inhibitors thus the betachain probably located near the alphachain on the cell surface was associated with a highaffinity class of gmcsf receptors | MEDAL |
an asymptomatic yearold woman on followup for a carotid body RT showed magnetic resonance imaging mri focal restricted diffusion confined to the left temporal and occipital cortices thirteen months later diffusionweighted images revealed a bilateral cortical ribbon sign involving all lobes after month the patient developed gait instability and cognitive decline rapidly evolving to severe dementia and death within months prion protein gene sequence molecular and neuropathological studies confirmed the diagnosis of sporadic creutzfeldtjakob disease scjd mm AT1 here we show the kinetics of mri changes and prion spreading in preclinical scjd mm ann neurol | MEDAL |
chickens were housed when day old with chickens experimentally infected with mycoplasma synoviae air sacs from of the weekold and eight of the weekold contactexposed chickens were given gross lesion scores and embedded in GMA for slide preparation histologic lesions in air sacs with gross scores of were mild edema resulting in a two to eightfold increase in air sac thickness capillary proliferation and exudate consisting largely of heterophils and necrotic debris histologic lesions in air sacs with gross scores of and were marked hyperplasia of epithelial cells and diffuse infiltration of the air sac connective tissue by MNC cells nine of air sacs with gross scores of had no histologic evidence of inflammation the most severe histologic lesions were in those air sacs with gross scores of the GMA procedure resulted in mum sections with excellent cellular detail | MEDAL |
the relationships of hladr and the newer ds second d locus b cell alloantigens mb and mt to the clinical and serologic expression of primary and AA forms of sjögrens syndrome ss were examined in patients whites and blacks although hladr was significantly increased in whites of and blacks of with pSS compared with racematched NCs it was not appreciably elevated in those with SLE erythematosus sless rheumatoid ICA rass or connective tissue diseasess the mt specificity however was more strongly associated with primary ss of whites and of blacks and also with sless and rass compared with racematched NCs furthermore mt was significantly increased in sless and rass when compared with nonsicca sle and ra controls although primary and AA ss were most strongly associated with this ds specificity mt the antiro ssa and antila ssb antibody responses were more closely allied to dr antigens DR was increased in antiro positive patients both whites and blacks with primary ss and in total antiro positive subjects compared with their antiro negative counterparts and respectively among dr negative patients hladr correlated with antiro in both primary ss and in the total ss group thus of ro antibody positive patients with primary ss had dr andor dr as did of antiro positive subjects in all categoriesabstract truncated at words | MEDAL |
MP commonly causes respiratory tract infections in humans but it may also be associated with CNS system manifestations the aim of the present T0 was to determine whether the CSF fluid taken from patients with neurologic PS due to MP infection contains TPS antibodies and whether the detection of these antibodies can be used for diagnosis mycoplasma pneumoniae was isolated from the CSF fluid taken from nine patients with CNS system PS on admission to the hospital in addition mycoplasma pneumoniae was detected in cerebrospinal fluid using PCR reaction in four other patients antibodies to MP were detected using the EIA indirect IPA and immunoblotting in cerebrospinal fluid samples from of patients included in the T0 the indirect immunoperoxidase assay showed high titers of mycoplasma pneumoniae immunoglobulin g igg and igm antibodies in CSF fluid samples of some patients with meningoencephalitis or meningitis titers of specific iga igg and EA were lower while TPS igg was not detectable CSF fluid samples with higher antibody titers also contained iga igg igg igg and igm antibodies that recognized the p adhesin kda protein of MP a comparison of antibody titers is the mean costs for the inpatient hospitalization were this is one of the first studies to document cost of icd therapy and may serve as a benchmark for other facilities | MEDAL |
we have used two methods to detect TPS transcription of the chicken alpha type i collagen gene in CF extracts derived from rous sarcoma virustransformed chicken embryo fibroblasts the first method is a modification of the s nuclease mapping procedure which utilizes a dna probe labeled with p at the end of the hindiii linker originally used to clone the collagen promoter region into pbr the probe distinguishes newly made specific rna from endogenous rna and nonspecific transcripts using this procedure we have found that chicken whole cell extracts support accurate initiation of transcription of the chicken alpha type i collagen dna template addition of either creatine phosphate gtp or utp to concentrations of approximately to mm was found to stimulate rna polymerase ii transcription by to fold the second method employs an avian myeloblastosis virus reverse transcriptasecatalyzed primary extension procedure rendered in vitrospecific by use of a pbr fragment as primer these two techniques should be useful for analyzing TPS transcription in other types of CF extracts | MEDAL |
neural activity in the auditory system decreases with repeated stimulation matching CS- probability in multiple timescales this phenomenon known as stimulusspecific adaptation is interpreted as a neural mechanism of regularity encoding aiding AEP object formation however despite the overwhelming literature covering recordings from singlecell to scalp auditoryevoked potential aep stimulation timing has received little interest here we investigated whether timing predictability enhances the experiencedependent modulation of neural activity associated with CS- probability encoding we used human electrophysiological recordings in healthy participants who were exposed to passive listening of sound sequences pure tones of different frequencies were delivered in successive trains of a VL number of repetitions enabling the study of sequential repetition effects in the aep in the predictable timing condition tones were delivered with isochronous interstimulus intervals in the unpredictable timing condition interstimulus intervals varied randomly our results show tha pasteurianum has the inherent potential to grow on glycerol and produce propanediol and butanol as the major products growth and product yields on crude glycerol were reported to be slower and lower respectively in comparison to the results obtained from pure glycerol in this T0 we analyzed the effect of each impurity present in the biodieselderived crude glycerol on the growth and metabolism of glycerol by c pasteurianum the crude glycerol contains methanol salts in the form of potassium chloride or sulfate and fatty acids that were not transesterified salt and methanol were found to have no negative effects on the growth and metabolism of the bacteria on glycerol the fatty acid with a higher degree of unsaturation LA acid was found to have strong inhibitory effect on the utilization of glycerol by the bacteria the fatty acid with lower or no degrees of unsaturation such as stearic and OA were found to be less detrimental to ATP utilization the removal of fatty acids from crude glycerol by acid precipitation resulted in a fermentation SMB that is comparable to the one on pure glycerol these results show that the fatty acids in the crude glycerol have a negative effect by directly affecting the utilization of glycerol as the carbon source and hence their removal from crude glycerol is an essential step towards the utilization of crude glycerol | MEDAL |
the outcome and the evolution of hostsymbiont associations depend on environmental constraints but responses are difficult to predict since they arise from a complex interaction between the host the parasite and the environment the situation can be even more complex when multiple parasite genotypes with potentially different responses to environmental changes coexist within a single host in this paper we investigated the effect of the temperature from to degrees c during the host OD on the density of strains of the intracellular bacterium wolbachia that coexist within the wasp leptopilina heterotoma in this species wolbachia induces cytoplasmic incompatibility a spermegg incompatibility that allows it to spread and persist in host populations using realtime QPCR we found that i wolbachia density is temperaturespecific and highest at degrees c ii the order of the abundance of the wolbachia strains does not vary with temperature changes iii the response of bacterial density to temperature occurs within a single insect generation during the eggtoadult developmental period iv in this species temperaturerelated changes in wolbachia density do not influence CI | MEDAL |
MAP map kinases are G1 by the sequential activation of ras raf and mek map kinase kinase and regulate a wide variety of cell functions to determine the kinase cascade for granulocytemacrophage colonystimulating factor gmcsf and ilinduced map kinase activation in eosinophils we studied the effect of inhibitors of jak kinase TKs phosphatidylinositol kinase and protein kinase c on gmcsf and ilinduced map kinase activation in human eosinophils gmcsf and il G1 and kilodalton map kinase isoforms in eosinophils this was indicated by the electrophoretic mobility shift of the three isoforms of map kinase in immunoblotting with antimap kinase antibody and also by ingel map kinase assay map kinase activation was time and dosedependent becoming maximal to minutes T3 stimulation a jak kinase inhibitor ag a IMT kinase inhibitor genistein and a phosphatidylinositol kinase inhibitor wortmannin inhibited gmcsf and ilinduced map kinase activation in eosinophils whereas a protein kinase c inhibitor staurosporine had a weak inhibitory effect furthermore ag and genistein prevented gmcsfinduced tyrosine phosphorylation of jak kinase in eosinophils taken together these results indicate that gmcsf and il activate map kinases through the signaling pathway of jak kinasetyrosine phosphorylated beta chainphosphatidylinositol kinaseras in eosinophils | MEDAL |
measuring tidal volume vt in nonintubated swine or swine with leaking PLB circuits is challenging the aim of this T0 was to validate RIP rip for measuring vt in swine that are comparable in size to adult humans to determine calibration curves vt and rip readings were obtained from anesthetized swine n weight kg during positivepressure mechanical ventilation and spontaneous breathing for positivepressure ventilation pigs were MV by using the pressurecontrol mode the pigs in the spontaneously PLB cohort each received a single intravenous bolus dose of propofol to abolish spontaneous PLB vt was measured during gradual return of their P0.1 a flowvolume sensor was placed between the proximal end of the endotracheal tube and breathing circuit for the recording of inspiratory and expiratory vt rip readings were recorded by using bands which simultaneously measured ribcage and ABD excursions the data revealed that vt was linearly correlated with the movements of both ribcage and abdomen as measured by using plethysmography over a large range of tidal volume to ml in addition the intercept of the linear equation was small or even negative during spontaneous breathing but increased significantly maximum ml ± ml during positive pressure ventilation our results indicate that vt in swine can be calculated by using a simple univariate linear regression equation with rip readings obtained during either mechanical ventilation or spontaneous PLB | MEDAL |
thermally induced dislocation movements are important in understanding the effects of high temperature annealing on modifying the crystal structure we use an in situ heating holder in an aberration corrected transmission electron microscopy to T0 the movement of dislocations in suspended monolayer graphene up to °c control of temperature enables the differentiation of EB induced effects and thermally driven processes at RT the dynamics of dislocation behavior is driven by the EB irradiation at kv however at higher temperatures increased movement of the dislocation is observed and provides evidence for the influence of thermal SE to the system an analysis of the dislocation movement shows both climb and glide processes including new complex pathways for migration and C1 nanoscale rapid jumps between fixoscopy was used as a process analysis technique in the synthesis of chloropropionate for the first time then the partial least squares regression plsr quantitative MM of the product solution concentration was established and optimized correlation coefficient r of partial least squares regression plsr calibration MM was and the root mean square error of correction rmsec was moll these values of plsr and rmsec could prove that the quantitative calibration model had good performance moreover the root MSE of prediction rmsep of validation set was moll the results were very similar to those of offline gas chromatographic analysis which could prove the method was valid | MEDAL |
prazosin an antagonist of alpha adrenergic receptors has been found to suppress the clinical and histological expression of exp autoimmune encephalomyelitis eae in the lewis rat suppression was more significant in females than in males and was a dosedependent phenomenon analysis of the effect of othalpha beta where beta represents a proteolytically derived fragment of the beta S1 this analysis of S1 composition was confirmed by silver staining of affinitypurified insulin receptor following resolution of the forms on a mono q column as described previously we have characterized the three isolated insulin receptor forms with regard to ligand IB by ligand and scatchard analysis we also measured insulinstimulatable autophosphorylation and exogenous kinase activity directed toward polyglutyr the three forms of the insulin receptor exhibit similar kds for insulin binding to the high and lowaffinity sites the alpha beta and alpha betaalpha beta forms of the insulin receptor display superimposable curvilinear scatchard plots in contrast only the IN holoreceptor alpha beta form demonstrates insulinstimulatable autophosphorylation and exogenous kinase activity the alpha betaalpha beta form has reduced basal kinase activity which was not increased by prior incubation with insulin the alpha beta form lacks a kinase domain and consequently demonstrated no kinase activityabstract truncated at words | MEDAL |
two seven and tendayold scwl cockerels were injected with pineal extract pe GTH releasing hormone gnrh and luteinizing hormone lh CT or in combination to determine the effect of pe on the action of exogenous gnrh and lh on the testis in immature cockerels 32P p uptake by the testis was used as an CA for GTH activity gnrh had no effects on cockerels aged two days however lh caused increased p uptake compared to the saline control at this age in seven and tendayold cockerels pe in combination with gnrh resulted in lower p cpmg of testis compared to the nonpe treated birds at the same doses of gnrh on the other hand pe did not cause any decrease in testis response to lh these results indicate that pe is acting to inhibit release of gonadotropins by gnrh | MEDAL |
the activity of identified cerebellar nuclear neurons was recorded in the alert cat during blinks induced by corneal air puffs CS flashes and tones eyelid response to air puffs consisted of an early ms downward movement followed by two to three late downward steps blinks induced by flashes or tones presented longer latencies and ms type a neurons n increased their spike activity in coincidence with the beginning of the blink regardless of the CS- modality the late eyelid downward responses were accompanied by corresponding increases in the firing rate of the neuron type a neurons were activated mostly from the RN or the restiform body type b SN n fired a brief burst of spikes slightly preceding the blink followed by a noticeable decrease in their FR as for type a the discharge response of type b neurons was always the same regardless of the sensory modality these SN were activated from the red nucleus oculomotor complex and restiform body although no precise temporal coupling was found between the beginning of the neuronal response and the start of either the stimulus or the motor response linear SRA demonstrated significant relationships between mean firing rate of type a and b SN and eyelid position velocity andor acceleration deep cerebellar nuclei neurons presented here seem to be directly involved in the execution of reflexively induced blinks following the smaller details of eyelid motor performance the opposite SMB of type a and b cells suggests an interplay of reciprocal actions to determine the ongoing displacements of the lid finally the cerebellum seems to influence blinks through a spread action on many BS CS and not exclusively on the red nucleus | MEDAL |
maternal food restriction fr during pregnancy results in decreased body weight with increased plasma leptin to address this paradox we investigated the effects of fr during pregnancy on growth and leptin C2 in maternal placental and fetal sites from embryonic day e control pregnant rats received ad libitum adlib food whereas study rats were fr at GAs e and e the alterations in maternal body composition retroperitoneal versus subcutaneous adipose leptin expression and plasma leptin C2 were studied furthermore these changes were related to nonpregnant np status and placentalfetal growth and leptin C2 as compared to np both fr and adlib dams showed a progressive increase in body and lean body mass however total body fat was reduced in fr dams but remained unchanged in adlib dams furthermore plasma leptin C2 in fr dams were markedly increased at e unlike adlib dams which showed moderate increments at e and e additionally fr dams showed significantly decreased OB expression in subcutaneous and notably unaltered levels in retroperitoneal adipose tissue suggesting an alternate source of elevated maternal plasma OB more importantly the fr dams had reduced placental weights with paradoxical increased leptin expression at both gestations thus increased plasma leptin C2 at e in maternal fr pregnancies may be explained in part by upregulation of placental leptin despite maternal and placental hyperleptinemia during fr pregnancies the growthrestricted fr fetus had reduced leptin C2 these findings have important implications for pregnancy outcome and fetal growth | MEDAL |
a robust literature has emerged on the dark triad dt of personalitymachiavellianism mach psychopathy and narcissism questions remain as to whether mach and psychopathy are distinguishable and whether machs empirical and theoretical networks are consistent in study n mturk research participants factor analyses were used to compare twofactor mach and psychopathy combined narcissism and threefactor models with both fitting the data equally well in studies and n undergraduate research participants dt scores were examined in relation to a variety of external criteria including self and informant ratings of personality adverse developmental experiences and psychopathological symptomsbehaviors in both studies mach and psychopathy manifested nearly identical empirical profiles and both were significantly related to disinhibitory traits thought to be antithetical to mach in T0 n expert raters expert ratings of the fivefactor model traits prototypical of mach were collected and compared with empirically derived profiles measures of mach yielded profiles that were inconsistent with the prototypical expertrated profile due to their positive relations with a broad spectrum of impulsivityrelated traits ultimately measures of psychopathy and mach appear to be measuring the same construct and mach assessments fail to capture the construct as articulated in theoretical descriptions | MEDAL |
the purpose was to investigate the rho between epicardial adipose tissue eat thickness eat volume and severity of coronary artery stenosis | MEDAL |
scarce data are available whether cardiac magnetic resonance cmr assessment of myocardial deformation provides independent and incremental prognostic information in patients with stsegment elevation myocardial infarction stemi the aim of the present study was to investigate the prognostic utility of cmr featuretracking derived left VVI lv global circumferential CS gcs in stemi patients | MEDAL |
induction by caerulein of AP with tissue damage and acinar cells loss is followed by REC we studied biochemical histological and PET regeneration of pancreatic tissue after repeated acute one pancreatitis was evoked in rats by sc infusion of caerulein microgramskgh for h T3 infusion rats were divided into three groups G1 was infused with caerulein one time in the second group infusion of caerulein was repeated days later the third CG was infused with caerulein for the rd time days after the nd infusion rats were sacrificed at time CS of hours and at th and th day T3 last infusion of caerulein pancreatic blood flow pbf was measured using laser doppler flowmeter plasma and pancreatic amylase pancreatic weight rna and dna contents and histological changes were determined we found that dna and rna content as well as histological changes in st group showed progressive regeneration T3 days regeneration T3 st time caeruleininduced pancreatitis was almost completed within days and amylase content in the tissue and plasma amylase level returned to normal values each subsequent infusion of caerulein caused significantly less pronounced destruction of the pancreatic tissue however the regeneration occurred progressively later than after the st or nd infusion tissue repair T3 the nd infusion reached peak at th day while T3 rd infusion at th day pbf dropped after st caerulein induced pancreatitis by about but with repeated caerulein induced pancreatitis lower decreases in pbf were observed and they returned in shorter time back to control value these results indicate that the pancreas is able to adapt to repeated injury and this is manifested by cumulative decrease of pancreatic damage T3 each repetition of induction of acute one and correlated with the preservation of pbf however the pancreatic tissue regeneration is significantly delayed | MEDAL |
the purpose of this T0 was to compare the accuracy required time and potential advantages of rapid prototyping technology with traditional methods in the manufacture of wax patterns for two facial prostheses | MEDAL |
atrial septal defect asd is a common congenital HR disease chd in humans but considered relatively rare in veterinary medicine however modern echocardiographic and doppler techniques currently offer a good view of the morphology of the interatrial septum thus facilitating earlier detection of asd in awake animals in this context we carried out a retrospective study of cases of recently diagnosed asd in dogs and cats at the cardiology unit of alfort using echocardiography combined with colour doppler mode asd was diagnosed in animals and represented of all canine and feline chds n asd was the most common chd T3 mitral dysplasia in both species boxer and domestic shorthair were the most common canine and feline breeds affected most defects were secundumtype asd without clinical signs in of cases the most common clinical signs included systolic murmur heard over the left HR base exercise intolerance syncope dyspnoea and cough animals that presented a systolic heart murmur over the left base had a significantly larger asd than others p these data suggest that the incidence of asd is higher than previously assumed asd should be suspected for example in instances of left basal systolic HR murmur although its clinical and haemodynamic consequences are usually minor | MEDAL |
the effect of prior treatment with anabolic steroids was studied in threeyearold mares in the preceding year these mares had been assigned to of treatment groups and had received the manufacturers recommended dosage of mg of boldenone undecylenate bukg of body weight mg of boldenone undecylenate bukg mg of nandrolone decanoate ndkg or ml of sesame oilkg control c mares had received an injection every weeks for weeks for a total of i.v. with the final injection in december in the following breeding season fewer p less than mares in all groups previously po anabolic steroids displayed estrous behavior than did mares in the control group duration of estrus was shortened p less than in mares that had received steroids abnormal sexual SMB that was observed during steroid treatment continued p less than for up to months after treatment ceased however observations of abnormal behavior declined with time p less than all mares in each treatment group ovulated by the end of the trial and the interval to first ovulation was similar p greater than ovarian size follicular OD and conditions of the tubular genitalia was adversely p less than affected in mares in all steroidtreatment groups until approximately the middle of march T3 that time no difference was noted among CG firstcycle PRs were and for mares in the untreated bu bu and nd CG respectivelyabstract truncated at words | MEDAL |
effects of prostaglandin e pge on ion transport were examined by observing the transmural vt and basolateral membrane voltage vb in the in vitro perfused rb connecting tubule cnt and the cortical collecting duct ccd addition of microm pge to the bath induced a biphasic response of transmural voltage vt with initial negative vt deflection followed by positive deflection in the cnt but monophasic negative deflection in the ccd because pge had no affect on the basolateral membrane Vc vb pge mainly causes changes in the apical membrane voltage elimination of na from the lumen abolished the pgeinduced vt response in the cnt in the presence of microm luminal amiloride pge caused only an initial negative deflection without causing later positive deflection the positive vt deflection induced by pge in the ccd was also blocked by luminal amiloride addition of ouabain mm to the bath completely abolished the pgeinduced vt changes in the cnt indicating that an intact nak pump is a prerequisite for the vt response to pge addition of mm ba to the lumen did not affect biphasic vt response to pge indicating that basensitive k conductance is not involved basolateral addition of mm chlorophenylthiocamp inhibited only the negative vt deflection induced by pge the positive vt deflection was blocked by basolateral addition of microm nndiethylaminooctyl trimethoxy benzoate hydrochloride tmb an inhibitor of intracellular ca release but beta of luminal ca did not affect the biphasic response to pge these findings suggest that the initial negative vt deflection is caused by an increase in na influx across the luminal membrane through an amilorideinsensitive na conductive pathway whereas the later positive deflection is caused by the inhibition of na influx through the amiloridesensitive na conductive pathway the camp messenger system may be responsible for the initial negative deflection whereas an increased intercellular ca release from the store is necessary for the later positive deflection caused by pge the response in the ccd is comparable to the later response in the cnt | MEDAL |
the aim of this study was to compare diagnostic performance of creactive protein crp and polyc avid ribonuclease prnase levels in the prediction of a severe clinical course of acute one ap the T0 included patients with mild and with severe ap crp concentration was measured by an immunonephelometric method and prnase activity by the rate of polycytidylate hydrolysis at ph at the time of admission both prnase and crp C2 were significantly increased in all patients when compared to HS vs ul and vs mgl p up to days and a further increase in prnase was observed on the other hand the increase in crp continued only through days and p severe acute one sap and mild acute pancreatitis map differed significantly with respect to prnase C2 on all days studied whereas crp C2 differed significantly on days but did not differ at admission receiver operating characteristic roc curve function analysis yielded the best sensitivity of sap PCD for prnase equaling at the cutoff point value ul on day after admission the sensitivity of crp for PCD of sap was at mgl on the nd day after admission both parameters studied were significantly associated with the severity of the ap clinical course however on days and postadmission prnase was more specific for detection of sap than crp vs on the st day and vs on the nd day in conclusion prnase has shown an excellent performance for early differentiation of acute necrotizing pancreatitis | MEDAL |
when murine lymphocytes were cultured in rpmi medium containing fetal calf serum fcs for more than d without added antigens suppressor t cells ts that suppressed in vitro antibody responses to sheep erythrocytes dinitrophenylficoll and trinitrophenyl tnplipopolysaccharide nonspecifically were induced spontaneously thus we failed to detect the activities of antigenspecific ts that are generated by priming the lymphocytes in vitro with a specific antigen under these experimental conditions to avoid the induction of nonspecific ts we examined various SC conditions and found that nonspecific ts were not induced only when a speparable doses of subcutaneous desferrioxamine the amounts of iron excreted were related to the dose of l administered and the iron load of the patients the urinary excretion of zinc magnesium and calcium did not increase and the drug was well tolerated | MEDAL |
GABA acid gaba was injected intraperitoneally ip in conscious restrained rats and the effects on core temperature were observed gaba mgkg produced a dosedependent decrease in core temperature at an AT of degree c gabainduced HT mgkg was att by i.c.v. with RES mgkg ip pchlorophenylalanine mgkg ip yohimbine mgkg ip or methysergide mgkg ip neither alphamethylptyrosine mgkg ip nor phenoxybenzamine mgkg ip nor PIM mgkg ip antagonized gabainduced hypothermia i.c.v. with either propranolol mgkg ip or BIC mgkg ip potentiated HT induced by gaba it is concluded that HT produced by ip i.t. of gaba could be due to release of serotonin by activation of bicucullineinsensitive gaba receptors located on central serotonergic SN | MEDAL |
under certain environmental conditions marine and FW phytoplankton may produce phycotoxins inhibitors of serinethréonine protein phosphatases a and in the marine environment dinoflagellates produce fatty polyethers okadaic acid and its derivatives the dinophysistoxins which accumulate in shellfish and can cause diarrhetic shellfish poisoning dsp when ingested in freshwater the toxins are microcystins and nodularin or CAA cyclic peptides and are hepatotoxic these toxins have caused massive poisoning of wild animals or domestic livestock and now are a health threat for humans through use of drinking and recreation water moreover all these toxins are potent RT promoters but belong to a new class different from the tpa class because they do not act on protein kinase c although the mutagenicity ames test responds negatively several results show their genotoxic potential and therefore they are a health hazard through chronic exposition to low doses finally okadaic acid through its easy penetration in all cellular types can be used as a tool to T0 mechanisms involved in protein phosphorylationdephosphorylation processes | MEDAL |
besides its caregulative effects calcitonin is known to diminish sensitivity to painful stimuli the present T0 aimed to clarify whether calcitonin has similar effects on CS- processing in other modalities effects of calcitonin were assessed on BB potentials recorded from the human scalp which were evoked either by AEP clicks or visual checkerboard patternreversals twelve healthy men were tested in a doubleblind intrasubject design receiving either iukg salmon calcitonin sct or iukg sct or saline solution during a min iv infusion sct significantly increased RL of wave v of the brainstem AEP evoked potential baep effects of baep wave v increased in magnitude with increasing dose of sct and with decreasing intensity of the click stimulus there was also a slight increase in RL of the n of the patternreversal visual CEP prvep additionally subjects rated themselves as less activated following the high dose of sct compared to PL the pattern of results is in accord with a slowing or GABA influence of calcitonin on auditory and visual sensory processing thus paralleling findings concerning calcitonin effects on the perception of painful somatosensory stimuli | MEDAL |
lead toxicity is closely related to its accumulation in several tissues and its interference with bioelements whose role is critical for several biological processes recently oxidative AS has been proposed as a possible mechanism involved in lead toxicity this study was carried out to investigate the effect of dosedependent lead exposure on haematological and oxidative AS parameters adult male WR g were divided into three CG group pb and group pb received respectively mg pb n and mg pbkg bw n as lead acetate solution ip for a period of seven days group t n served as control and received mg nakg bw as sodium acetate solution ip for the same period all animals were sacrificed h after the last injection blood superoxide dismutase sod and blood glutathione peroxidase gpx MICs and plasma bilirubin level were measured CL was quickly excised for the estimation of alteration in lipid peroxidation indices mda lead SE induces in both treated groups a marked decline in haematocrit and haemoglobin levels p when compared to control the results show also a significant decrease p in sod activity but only in group pb sod activity did not decrease in group pb in comparison with control p however lead SE caused a light increase in gpx activity in group pb which remains nonsignificant p compared to control group pb did not record significant changes in the activity of gpx lead exposure for a period of seven days resulted in a significant p increase in bilirubin level in group pb compared to control the bilirubin level from rats of group pb did not reach a statistical significance changes in CL mda content in leadexposed rats from pb and pb groups did not reach a statistical p significance it is concluded that lead induces oxidative stress in a dosedependent manner no dosedependent response was observed in blood gpx activity and CL mda content these results could be due to the short duration of the treatment | MEDAL |
a MC ML system is described that facilitates the in vitro cultures of human spontaneous tumor cells the tumor samples were disaggregated mechanically and cultured in methylcellulose with fetal CS in iscoves modified dulbeccos medium eightyfive individual tumor samples with different histological types were seeded gave rise to tumor cell colonies the plating efficiency ranged from to cytologic and cytochemical IF from aspirated cells revealed the same morphology of cells as the cells in the suspension a flattening of colonies as it has been described by others using methylcellulose ML was not observed | MEDAL |
the effects of in vitro SC systems for sheep zygotes on subsequent fetal growth and development to day and day of gestation were studied zygotes recovered from superovulated scottish blackface ewes approximately h after intrauterine insemination using semen from a single suffolk sire were cultured for days in a a granulosa cell coculture system coculture b synthetic oviductal fluid medium without SS sof and c synthetic oviductal fluid medium supplemented with human SS sof control embryos were recovered from superovulated donor ewes at day T3 oestrus embryos were transferred at day to synchronous scottish blackface recipient ewes in total gravid uteri were recovered comprising at day coculture sof sof and control and at day coculture sof sof and control of gestation fetuses derived from cocultured embryos were heavier p by day of gestation than those derived from control embryos growth coefficients derived from the linear allometric equation logey logea b logex where y organ mass x fetal mass were significantly greater p for liver HR kidneys and plantaris muscle in fetuses derived from cocultured embryos and for liver in fetuses derived from sof embryos than those for control fetuses fetuses derived from cocultured embryos were heavier p and fetuses derived from sof embryos were heavier p by day of gestation than those derived from control embryos growth coefficients for CL and HR for fetuses derived from coculture and sof embryos were also significantly greater p at this stage of gestation than those for control group fetuses in contrast allometric coefficients for these organs in fetuses derived from embryos cultured in sof without SS supplementation were not different from those for controls excessive volumes of amniotic fluid polyhydramnios were observed in of conceptuses derived from cocultured embryos in vitro embryo culture can significantly influence fetal growth and this T0 provides quantitative evidence of L1 shifts in the patterns of organ and tissue OD | MEDAL |
fty is a novel IS agent that is thought to reduce the number of IP blood lymphocytes pbl by directing them toward secondary lymphoid organs such as the lymph NO and PPs we studied the effects of fty on alyaly mice that do not have either CL nodes or peyers patches as well as on splenectomized alyaly mice | MEDAL |
women who selfselect to take postmenopausal hormones have lower risks of coronary HR disease their leading cause of mortality women and their PHC providers must weigh this and other clear osteoporosis and possible stroke CC and alzheimers disease benefits against clear endometrial CA and possible breast CA risks because all existing data derive only from observational studies reliable information on the balance of risks and benefits must await the results of the womens health initiative a largescale randomized CT | MEDAL |
among individuals with a history of myocardial infarction mi higher levels of blood pressure bp are associated with increased LT risks of death from coronary HR disease treatment with a bplowering regimen based on omapatrilat may result in greater clinical benefits than treatment with a regimen based on a regular angiotensinconverting enzyme ace inhibitor because of more favourable effects on the reninangiotensinaldosterone system | MEDAL |
several recent studies have examined different aspects of mammalian higher order chromatin structure replication timing lamina association and hic interlocus interactions and have suggested that most of these features of genome organisation are conserved over evolution however the extent of evolutionary divergence in higher order structure has not been rigorously measured across the mammalian genome and until now little has been known about the characteristics of any divergent loci present here we generate a dataset combining multiple measurements of chromatin structure and organisation over many embryonic cell types for both human and mouse that for the first time allows a comprehensive assessment of the extent of structural divergence between mammalian genomes comparison of orthologous regions confirms that all measurable facets of higher order structure are conserved between human and mouse across the vast majority of the detectably orthologous genome this broad similarity is observed in spite of many loci possessing cell type TPS structures however we also identify hundreds of regions from kb to mb in size showing consistent evidence of divergence between these species constituting at least of the orthologous rat genome and encompassing many hundreds of human and mouse genes these regions show unusual shifts in human gc content are unevenly distributed across both genomes and are enriched in human subtelomeric regions divergent regions are also relatively EC for genes showing divergent expression patterns between human and mouse es cells implying these regions cause divergent regulation particular divergent loci are strikingly EC in genes implicated in vertebrate OD suggesting important roles for structural divergence in the evolution of rat developmental programmes these data suggest that though relatively rare in the rat genome divergence in higher order chromatin structure has played important roles during evolution | MEDAL |
a risk score for atrial fibrillation af has been developed by the framingham heart study and cohorts for HR and aging research in genomic epidemiology chargeaf consortium however validation of these risk scores in an innercity population is uncertain thus a validation MM was built using the framingham risk score for af and chargeaf covariates an in and OP electrocardiographic DB was interrogated from to for the OD of af patients were included if their age was and years had year followup if their initial electrocardiogram was without af had ⥠electrocardiograms and declared a race andor ethnicity as nonhispanic white africanamerican or hispanic for the framingham heart study patients met inclusion criteria of which developed af discrimination analysis using area under the curve auc for original risk equations nonhispanic WG auc confidence interval ci to africanamerican auc ci to and hispanic auc ci to for the chargeaf patients met inclusion criteria of which developed af nonhispanic WG auc ci to africanamerican auc ci to and hispanic auc ci to calibration analysis showed qualitative similarities between cohorts in conclusion this is the first study to validate both the framingham heart T0 and chargeaf risk scores in both a hispanic and africanamerican cohort all models predicted af well across all race and ethnic cohorts | MEDAL |
the mitochondrial carrier family transports a variety of metabolites across the inner mitochondrial membrane we identified and cloned a new member of this family kmcp kidney mitochondrial carrier protein that is highly homologous to the previously identified protein bmcp BB mitochondrial carrier protein WB and in situ experiments showed that this carrier is expressed predominantly within the kidney cortex in the proximal and distal tubules kmcp was increased during fasting and during the regenerative phase of glycerolinduced renal failure we show that both situations are associated with transiently increased expression of superoxidegenerating enzymes followed by increased mitochondrial metabolism and antioxidant defenses given that kmcp expression occurs simultaneously with these latter events we propose that kmcp is involved in situations in which mitochondrial metabolism is increased in particular when the cellular redox balance tends toward a prooxidant status | MEDAL |
this paper analyzes biometrical and biotypological practices of the biometric cabinet of the escola de educação fÃsica do exército physical education school of the army in rio de janeiro in the s and s published in revista de educação fÃsica do exército it was necessary to classify monitor the results of the exercises periodically and measure morphological aspects of the bodies the classifications were made in accordance with foreign parameters and an attempt was made to classify by type SQ and defects the analysis of the practices of the cabinet shows that biometrics and biotypology were complementary aspects of PCS education aiming at the standardization of HB | MEDAL |
acute lung injury ali acts as a complex genetic trait yet its genetic risk factors remain incompletely understood largescale genotyping has not previously been reported for ali | MEDAL |
trichotillomania ttm is a disorder characterized by recurrent episodes of hair pulling that affects a growing and diverse patient population the SMB is a result of conscious or unconscious stimuli aimed at alleviating AS ttm can be diagnosed typically by a psychiatrist or dermatologist with various assessment tools and scales although researchers continue to discover new pharmacologic regimens and nonpharmcarried out to quantitatively T0 seasonal and solvent composition effects as well as their interactions on AA metabolites the mixture design consisted of ethanol AC dichloromethane and chloroform solvents and their binary ternary and quaternary mixtures yields were measured for the crude extract and its neutral organic basic polar and fiber fractions obtained with each solvent composition from samples harvested during twoway anova found statistically significant seasonal and solvent effects for the CE and all the fractions except for the polar fraction solvent the best solvent extractor depends the harvest season high crude organic and polar fraction yields in the summer are correlated with the coumarin uvvis absorbance at nm crude yields of mixtures containing ethanol are correlated with the coumarin absorbances in summer winter and spring whereas mixtures without ethanol show no significant rho | MEDAL |
in porcine studies anodes in the middle cardiac vein compare favorably with those in the rv it has not been demonstrated whether the rv and middle cardiac vein or the MCV CT anodes are superior when shocking to a conventional svc and active housing cathode nor whether a bystander MCV electrode exerts a passive electrode affect twelve pigs were anesthetized and had an AS housing implanted in the left pectoral region and defibrillation coils placed at the rv apex and in the svc a custommade defibrillation coil ela medical was advanced into the middle cardiac vein through a fr transvenous catheter the dft for three anodes rv rv and MCV MCV to the svc and AS housing was then assessed by a three reversal binary search the order of testing was randomized in seven animals dft was assessed in the same way for the configuration of rv to svc and AS housing twice more with and without a bystander MCV coil electrode in place the results were MCV j rv and MCV j reduced dft significantly compared to rv j both p there was no significant difference between the MCV and the MCV and rv p ci for difference the dft of rv to svc and the active housing was the same with and without the MCV bystander coil in place p ci for difference j shocking to a svc and active housing cathode middle cardiac vein and rv and middle cardiac vein anodes are equally ERP in lowering dft compared to the rv the MCV coil electrode does not exert a passive electrode affect on the rv to the svc and AS housing defibrillation | MEDAL |
the term bronchiolitis refers to a broad morphologic spectrum of inflammatory events that are centered on small conducting airways bronchiolitis may be an isolated pathologic finding although it is often a secondary consequence of diseases affecting other parts of the conducting apparatus or pulmonary acinus divergent causes of bronchiolitis may have similar microscopic findings hence a pathologic diagnosis is nonspecific and not clinically meaningful unless placed in the context of relevant clinical and radiographic findings etiologically most cases of bronchiolitis are infectious in nature or related to smoking increasingly other causes of bronchiolitis have been recognized including collagen V1 disease idiopathic inflammatory bowel disease or toxinsdrugs many cases are idiopathic histologically most examples of bronchiolitis demonstrate acute inflammation usually corresponding to viral infection other welldefined histologic categories of bronchiolitis include bronchiolitis obliterans in which there is fibroblast proliferation within airspaces and follicular bronchiolitis corresponding to lymphoid PH with formation of germinal centers in recent years several new forms of bronchiolitis have been recognized including constrictive bronchiolitis DPB and airwaycentered fibrosis this article highlights the clinical and pathologic features of these more recently described entities | MEDAL |
the relationships between reaction time rt and evoked potentials were investigated in three monkeys macaca nemestrina during the performance of a simultaneous brightness discrimination task evoked potentials from the lateral geniculate medial and inferior pulvinar midbrain reticular formation hippocampus and striate and prestriate SC were recorded concurrently with rt in response to the discriminative stimuli an early component of the geniculate response and a late positive wave in striate cortex were found to be related systematically to rt these components were largest for short rts and diminished progressively in amplitude as rt lengthened the statistical significance of these relationships was supported by a detailed analysis of single trials the increases in amplitude of evoked potentials associated with short rts were attributed to increased arousal level and were discussed in terms of reticular formation modulation of CE excitability C2 | MEDAL |
to evaluate factors affecting IP blood hematopoietic stem cell yield in patients undergoing largevolume leukapheresis for autologous peripheral blood stem cell collection | MEDAL |
cell cycle progression was studied in SF batch cultures of spodoptera frugiperda sf insect cells and the implications for proliferation and productivity were investigated cell cycle dynamics in kbm serumfree medium was characterized by an accumulation of of the cells in the gm phase of the Tc during the first h after inoculation following the Tc arrest the cell population was redistributed into g and in particular into the s phase maximum rate of proliferation micron max was reached h after the release from cell cycle arrest coinciding with a minimum CSD of cells in the gm phase the following declining micron could be explained by a slow increase in the gm cell population however at approximately h an abrupt increase in the amount of gm cells occurred this switch occurred at about the same time point and cell density irrespective of medium composition and maximum cell density an octaploid population evolved from gm arrested cells showing the occurrence of endoreplication in this cell line in addition CS medium AF were found to increase micronmax decrease the time to reach micronmax and decrease the synchronization of cells in gm during the lag and growth phase a CS medium factor appears to be a small peptide on basis of these results we suggest that the observed Tc dynamics is the result of autoregulatory events occurring at key points during the course of a SC and that entry into mitosis is the target for regulation infecting the sf cells with recombinant BV resulted in a linear increase in volumetric productivity of betagalactosidase up to h of culture beyond this point almost no product was formed medium renewal at the time of infection could only partly restore the lost hypertrophy and product yield of cultures INF after the transition point the critical time of infection correlated to the time when the mean population cell volume had attained a minimum and this occurred h before the switch into the gm phase we suggest that the cell density dependent decrease in productivity ultimately depends on the autoregulatory events leading to gm Tc arrest | MEDAL |
sulforaphane an organosulfur compound present in cruciferous vegetables has been shown to exert neuroprotective effects in experimental in vitro and in vivo models of neurodegeneration to determine whether sulforaphane can preserve cognitive function we examined its effects on scopolamineinduced SM impairment in mice using the morris water maze test sulforaphane or mgkg was administered to B6 mice by p.o. for days days and memory impairment was induced by intraperitoneal injection of scopolamine mgkg for days days mice that received scopolamine CT showed impaired learning and SM retention and considerably decreased cholinergic system reactivity in the hippocampus and FC SC as indicated by a decreased acetylcholine ach level and an increased acetylcholinesterase ache activity sulforaphane significantly att the scopolamineinduced SM impairment and improved cholinergic system CR as indicated by an increased ach level decreased ache activity and increased choline AT chat expression in the hippocampus and frontal cortex these effects of sulforaphane on ACh system CR were confirmed in vitro sulforaphane or μm increased the ach level decreased the ache activity and increased chat expression in scopolaminetreated primary cortical SN these observations suggest that sulforaphane might exert a significant neuroprotective effect on ACh deficit and cognitive impairment | MEDAL |
the cellular prion protein prpc is a glycoprotein anchored by GPI gpi to the cell surface and is abundantly expressed in the CNS system it is also expressed in a variety of cell types of the IS we investigated the role of prpc in the phagocytosis of apoptotic cells and other particles macrophages from mice with deletion of the prnp gene showed higher rates of phagocytosis than wildtype macrophages in in vitro assays the elimination of gpianchored proteins from the cell surface of macrophages from WT rendered these cells as efficient as macrophages derived from KO mice in situ detection of phagocytosis of apoptotic bodies within the retina indicated augmented phagocytotic activity in KO mice in an in vivo assay of acute peritonitis KO mice showed more efficient phagocytosis of zymosan particles than wildtype mice in addition leukocyte recruitment was altered in KO as compared with wild type the data show that prpc modulates phagocytosis in vitro and in vivo this activity is described for the first time and may be important for normal macrophage functions as well as for the pathogenesis of prion diseases | MEDAL |
preventive dentistry is mainly concerned with caries and periodontal disease and little or no attention is paid to the prevention of alveolar bone loss an overdenture contributes to the preservation of alveolar bone and offers a number of advantages in comparison to a conventional complete denture after tooth extraction the atrophy of edentulous lower jaws can be prevented or delayed by using implants supporting an overdenture or a fixed mandibular prosthesis hydroxyapatite implants have been studied as submerged tooth root substitutes and have proven to be able to preserve the bulk of the AM ridge a drawback of this submucosal implant is that the ridge maintenance depends solely on the PCS presence of the hydroxyapatite implants if implants support an overdenture or a fixed prosthesis they also play a role in maintaining the function of the bone in the different stages of reduction of the mandible in this paper a classification for the different resorption stages of the mandible is presented and is used to determine the correct moment for preventive implantology in addition the use of a lingualised occlusion is discussed as a contribution to the preventive goals in oral implantology | MEDAL |
the L1 human EM SGP GP a or mn glycoprotein was purified from the erythrocytes of two individuals heterozygous for the miviii gene in the miltenberger subsystem of the mnss bloodgroup system the CR structure of a tryptic glycopetide from GP a comprising the residues was deduced from automated and manual CS analyses the miviiispecific GP a was found to exhibit an argininethreonine exchange at position the threonine residue was found to be glycosylated hemagglutination and hemagglutination inhibition assays demonstrated that one of the miviiicharacteristic antigenic determinants anek is located within the residues of glycophorin a furthermore erythrocytes from the two miviii heterozygotes reacted only weakly with antienaktsera suggesting that the miviiispecific glycophorin a does not express the enakt antigen that is located within the positions of normal glycophorin a our data suggest that the miviiispecific GP a represents the evolutionary link between normal glycophorin a and the miviiispecific molecule which exhibits argininethreonine and tyrosineserine exchanges at the positions and respectively our data also provide an explanation for the close serological similarity between mivii and miviii erythrocytes | MEDAL |
this study evaluated the behavioral elements of three htrelated syndromes intraperitoneal hydroxytryptophan T3 intracisternal dihydroxytryptamine dht pchloroamphetamine pca fenfluramine ff or combinations of drugs scored from videotapes and their relationship to locomotor activity lma photocell recording regional MA concentration and s receptor IB rearing was eliminated by drugs which produce the myoclonic syndrome and was the single best indicator of control treatments saline or htp in unlesioned rats and saline in dhtlesioned rats global abnormality hunching rigid arching of back hindlimb abduction forepaw myoclonus stereotyped lateral head movements backing and immobility occurred significantly only in drugtreated rats multiple forms of myoclonus appendicular and truncal and convulsions were dosedependent drug effects both htp after dht and pca increased lma significantly but hyperactivity induced by pca could be blocked by giving htp concomitantly substantial ht presynaptic destruction by dht prevented backing but not other behavioral or locomotor effects of ff and pca drug combinations did not produce additive behavioral effects backing immobility and locomotor activity best differentiated between drug treatments and could be used to correctly allocate animals to drug CG drug treatments also could be differentiated by reducing the number of behavioral variables into summary variables PCs and by DA only forepaw myoclonus and total behavioral score were correlated with ht concentrations brainstem indicating behavioral heterogeneity our study suggests that there is a common core myoclonicserotonergic syndrome forepaw myoclonus head weaving hindlimb abduction hunching of stimulation of ht receptors plus additional drugspecific elements backing lma although BS receptors appear to be an important locus for some of these behaviors s receptors do not explain the behavioral supersensitivity to htp in our dhtlesioned rats | MEDAL |
preoperative BT bt does not correlate with postoperative bleeding in patients subjected to surgical procedures a significant positive rho has been reported between the bt hours after cardiopulmonary bypass surgery and the nonsurgical blood loss during the first hours after bypass surgery this T0 was done to investigate the effect of hct and platelet count on the bt measurement in normal healthy men and women | MEDAL |
cryopreservation of tissueengineered human dermal replacement plays an important role in skin tissue engineering and skin banking with the inspection of electronic scanning microscope and viability evaluation by TB staining CA and the tetrazolium salt mtt dimethylthiazolyldiphenyltetrazolium bromide CA this T0 investigated the toxicity of meso to dermal fibroblasts and effects of cryoprotectant concentration and cooling rate on the viability of dermal replacement the results demonstrated that the meso toxicity to fibroblasts was affected by the exposure time temperature and concentration furthermore adding cryoprotectant solution at LT of degrees c significantly reduced the toxic effect on the tissueengineered DE an optimal cryopreservation protocol consisting of cooling rate at degrees cmin in vv meso was derived with the viability of studied DE treated by this protocol being of that of fresh control the micrograph obtained by electronic scanning TSM also confirmed this result | MEDAL |
distribution of cerebellothalamic neurons projecting to the VP nuclei of the thalamus was examined in the cat using the horseradish peroxidase hrp method after injections of hrp within the lateral or VL portions of the ventroanterior and VL nuclear complex of the TH vavl neurons labeled retrogradely with hrp were seen contralaterally in the cerebellar nuclei many of them were situated in the NIA and NIP and a moderate number of them were located in the nucleus lateralis labeled SN in the NIP were observed mainly in the medial and VP portions of the nucleus on the side ipsilateral to the injections a few labeled SN were seen in the NIA NIP and nucleus lateralis virtually no labeled SN were found in the nucleus medialis of the cerebellum T3 hrp injections into the medial or dorsomedial portions of the vavl many labeled neurons were found contralaterally in the ventral and ventrolateral portions of the NIP as well as in the nucleus lateralis especially in its ventral and lateral portions on the side ipsilateral to the i.v. labeled SN in the nucleus lateralis and nucleus interpositus posterior were small in number in the nucleus medialis only a few labeled neurons were found bilaterally in the caudal C2 of the nucleus after hrp injections centered on the ventromedial nucleus of the TH many labeled SN occurred bilaterally in the caudal portions of the nucleus madialis with a slight contralateral preponderance and contralaterally in the lateral and VP portions of the nucleus lateralis a few labeled neurons were also seen contralaterally in the VL and lateral portions of the IP posterior and ipsilaterally in the nucleus lateralis | MEDAL |
to evaluate the relationships between changes in muscle morphology and metabolic adaptation to PCS training in obesity twenty obese women were subjected to a PT programme with three sessions a week for months physical training resulted in lowering of PI and improved glucose tolerance neither body weight nor body fat changed with PCS training the percentage distribution of fast twitch oxidative fta muscle fibres m vastus lateralis increased from to p less than and that of fast twitch glycolytic fibres decreased from to p less than the number of capillaries increased mainly around slow twitch st MF from to p less than and FT oxidative fta MF from to p less than the activities of oxidative enzymes cytochromecoxidase and citrate synthase increased p less than while those of glycolytic enzymes phosphofructokinase and hexokinase decreased T3 PT p less than significant negative correlations between PI and number of capillaries in contact with st fibres r p less than and fta fibres r p less than were found before training the capillary density around those fibres could predict of the explained variance of plasma insulin C2 p less than the changes of glucose concentration T3 training could be predicted by observed changes in enzyme activities the strong associations between muscle morphology and capillarization and enzyme activities and glucose and insulin concentrations and their changes T3 training suggest an important regulatory role of muscle which warrants further studies | MEDAL |
thirtythree patients with advanced refractory germinal neoplasms were treated with vp alone or in combination with cisplatinum bleomycin and frequently adriamycin all had prior chemotherapy which in most was extensive there were complete and partial remissions five of the patients with partial remission were rendered disease free by resection of residual tumor eleven remain disease free from months toxicity was considerable onethird were hospitalized for fever and granulocytopenia and four had lifethreatening TP there was one drugrelated death vp is an AS drug in germinal neoplasms it appears to be an important component of salvage regimens for refractory patients but severe toxicity may ensue | MEDAL |
the effects of sustained eucapnic hypoxia seh T2 sao approximately on ventilation and supraglottic airflow resistance rua plus genioglossal gg and diaphragmatic di electromyograms emgs were compared during wakefulness and nonrapid EM nrem sleep in six healthy normal male subjects early augmentation of ventilation was followed by decline or rolloff in both states the augmentation of ventilation was less in sleep than wakefulness eg after T2 hypoxia and of baseline respectively p this appeared to be due to three AF sleeprelated increases in rua the ventilatory responses to seh sleep vs awake were inversely related to changes in rua sleep vs awake p reduced central neural drive inspiratory phasic emg di after T2 seh and of baseline p and failure to increase RF during seh sleep there was also a NS trend to a biphasic response in emg gg and a small increase in rua during seh | MEDAL |
clinical research is important to improve medical SQ and ethics review is essential to conduct clinical research since the establishment of the first japanese ethics EC at the university of tokushima in japanese ethics committees have increased in this study we surveyed the status of clinical studies and ethics committees in one japanese region the survey was conducted in collaboration with the tokushima medical association a questionnaire was established and mailed to all medical institutions n registered to the tokushima medical association in among questionnaires were returned and were completed and are included in this analysis respondents among respondents had performed clinical research and of these had established ethics committees though one was omitted from the following analysis due to an unsatisfactory response among HECs review of protocol amendments review of serious adverse events annual followup of approved protocols and education for EC members were active in and respectively research ethics education was active in based on the results we attempt to establish an appropriate system for ethical conduct of healthrelated research in tokushima prefecture | MEDAL |
the aim was to T0 the apoptotic induction effect of thapsigargin on leukemia cell line k and its possible mechanism T3 the treatment of leukemia cell L1 k by thapsigargin morphological change of AI cells was investigated by aoeb FL IF under FL TSM apoptosis rate was determined with annexin vfitcpi double staining by flow cytometry intracellular calcium concentrations cai were measured by fluorescence spectrophotometer with calcium sensitive fluorescence indicator furaam mitochondrial transmembrance potentials delta psi m was detected on flow cytometry through staining of Rh rh the changes of ICE cytochrome c grp proteins were detected by western blot the results showed that k cells cultured in micromoll thapsigargin for hours exhibited typical morphological changes of AI cells under fluorescent TSM including shrinkage of cell condensation of chromatin breakage of nuclear formation of AI bodies fluorescence of yellow green and pellet observed in early apoptoyic cells and hyacinth fluorescence of chromatin showed in late AI cells and hours T3 SE to micromoll thapsigargin the AI rates of k were respectively and in dosedependent manner and were statistically significant when compared with the controls p the apoptotic rate of k was dose and timedependent in experiment range the enhancement of cai and the decrease of the delta psi m in k cells were induced by thapsigargin and were dosedependent in experiment range compared with control p western blot results indicated that cleavage and activation of caspase releasing of cytochrome c from mitochondria upregulation of grp expression at the endoplasmic reticulum were induced in k cells T3 hours exposure of micromoll thapsigargin it is concluded that thapsigargin induces endoplasmic reticulum stressinduced apoptosis in k cells endoplasmic reticulum is a novel important initiatory site of apoptosis in cells the cleavage and activation of ICE play very important role in endoplasmic reticulum stressinduced apoptosis of k cells and is one of the important mechanisms for thapsigargininduced apoptosis thapsigargininduced apoptosis in k cells is associated closely with the disruption of the delta psi m and the PR of cytochrome c from mitochondria mitochondria participates in endoplasmic reticulum stressinduced apoptosis in k cells | MEDAL |
we compute exact asymptotic results for the probability of the occurrence of large deviations of the largest smallest eigenvalue of random matrices belonging to the gaussian orthogonal unitary and symplectic ensembles in particular we show that the probability that all the eigenvalues of an nxn random matrix are positive negative decreases for large n as approximately exp beta thetan where the dyson index beta characterizes the ensemble and the exponent thetaln is universal we compute the probability that the eigenvalues lie in the interval zetazeta which allows us to calculate the joint probability CSD of the minimum and the maximum eigenvalue as a byproduct we also obtain exactly the average density of states in gaussian ensembles whose eigenvalues are restricted to lie in the interval zetazeta thus generalizing the celebrated wigner semicircle law to these restricted ensembles it is found that the DOS generically exhibits an inverse squareroot singularity at the location of the barriers these results are confirmed by numerical simulations some of the results presented in detail here were announced in a previous paper d s dean and s n majumdar phys rev lett | MEDAL |
mr f received several different treatment regimens for mm he required a cardioprotectant dexrazoxane to continue treatment with the vad regimen he received interferon highdose chemotherapy with tandem SC transplants and newer agents used in the treatment of mm such as thalidomide and TPT however as illustrated by mr fs case T0 mm remains an incurable disease despite advances in its treatment | MEDAL |
to investigate the action of the neck accessory muscles on the RC we stimulated the SCM and the scalenus muscles separately in supine anesthetized dogs hooks screwed into the sternum and ribs were used to measure their axial displacements and the changes in anteroposterior ap and transverse t diameters of the rib cage we found that the SCM and scalenus muscles when they contract CT cause a large axial MSD of the sternum and the ribs in a cephalad direction and expand the RC along both its ap and t diameters opening the abdomen increased the cephalad MSD of the ribs and the expansion of the lower rib cage particularly along its t diameter but reduced the increase in lung volume these experiments indicate that the action of the SCM and scalenus muscles on the RC is essentially the consequence of a rotation of the ribs neck axes resulting from the cephalad MSD of the ribs and that the fall in abdominal pressuru gene complex content the number of mast cells increases with age in both wild and in nu gene complex carriers nu and nunu the increase in the number of mast cells is faster in the nu gene carriers than in wild animals devoid of this gene in nude mice having T3 hepatitis a dramatic increase in the number of lymphoid mast cells was observed results obtained deny the importance of thymus in the production of lymphatic mast cells in mice | MEDAL |
humatergic SN emerged instead conversely ectopically expressed helt suppressed glutamatergic formation and induced gabaergic neurogenesis however the helt mutants showed normal progenitor domain formation in consequence postmitotic expression of the homeodomain factor nkx which was specifically expressed by gabaergic populations in wildtype embryos was maintained despite the transmitter phenotype conversion from gabaergic to glutamatergic in the helt mutants suggesting that helt is not involved in neuronal identity specification furthermore we identified proneural genes ngn and ngn which were selectively expressed in glutamatergic progenitors in the developing mesencephalon and had the ability to confer the glutamatergic fate as downstream target genes of helt these results suggest that helt determines gabaergic over glutamatergic fate at least in part by repressing ngn neurog genes and that basic HLH transcription factor networks involving helt and ngns are commonly used in the mesencephalon for determination of the gabaergic versus glutamatergic transmitter phenotype | MEDAL |
the purpose of this investigation was to examine the effects of days of inactivity ia on glucose tolerance gt resting VO2 rmr TEM tem and limb blood flow in endurancetrained men eight highly trained peak o consumption ml kg min endurance athletes participated in this T0 involving two MZ test days one approximately h after a normal training bout tr and the second after days of ia the following tests were conducted at each visit g oral gl tolerance test ogtt rmr and tem and measurements of calf and forearm blood flow bf by using venous occlusive plethysmography body weight remained unchanged during this short period of ia tr kg ia kg the area under the glucose and insulin curves increased tr vs ia mg dl min and tr vs ia microu ml min after ia respectively p rmr decreased significantly vs kcalmin p and RER during the ogtt increased vs p after ia whereas tem increased similarly in the tr and ia states in the tr state mean calf bf increased by vs ml ml min p during the ogtt but remained unchanged T3 ia whereas no differences at rest or during ogtts existed between the two conditions for forearm bf incremental insulin area above fasting during the ogtt was correlated with mean calf bf in the tr r p and ia r p states in conclusion days of ia results in a deterioration in gt and a reduction in rmr T3 gl ingestion calf bf was elevated compared with resting levels in the tr state but was unchanged in the ia state however limb bf was not related to gt or rmr thus our findings raise questions regarding the relative contribution of bf in modulating glucose tolerance and energy expenditure in endurance athletes in their habitual tr or ia state | MEDAL |
addition of betalapachone to the epimastigote SC form of trypanosoma cruzi suspended in saline bufferedisotonic medium ph determined the appearance of C1 amounts of ho in the suspension medium as measured spectrophotometrically by formation of the ho horse radish peroxidase complex under similar conditions alphalapachone did not induce ho formmation using nadh as electron donor betalapachone not alphalapachone increased significantly the rate of ho generation by epimastigote homogenates and the same occurred with nadph although in a reduced extent similar results were obtained with the isolated mitochondrial and microsomal fractions although with the latter nadph was more effective than nadh as electron donor for betalapachone reduction and peroxide generation the CSD of peroxide generation in epimastigote fractions would indicate that about of the betalapachone dependent formation of peroxide occurred in the mitochondria and in the endoplasmic reticulum the growth of epimastigotes was inhibited by microgramml betalapachone a concentration that determined Vmax of ho production since ho and other intermediates of oxygen reduction such as o superoxide anion and oh hydroxyl radical are lethal to cells and tissues it is possible that the effect of betalapachone on t cruzi proliferation in vitro was mediated by ho and related FRs | MEDAL |