text
stringlengths 10
10.1k
| source
stringclasses 1
value |
|---|---|
exercise is an effective MI for the prevention and treatment of type diabetes skeletal muscle combines multiple signals that contribute to the beneficial effects of exercise on cardiometabolic health inorganic nitrate increases exercise efficiency tolerance and performance the transcriptional regulator peroxisome proliferatoractivated receptor γ coactivator α pgcα coordinates the exercisestimulated skeletal muscle fibertype switch from glycolytic fasttwitch type iib to oxidative slowtwitch type i and intermediate type iia fibers an effect reversed in insulin resistance and diabetes we found that nitrate induces pgcα expression and a switch toward type i and iia fibers in rat muscle and myotubes in vitro nitrate induces the release of exercisepgcαdependent myokine fndcirisin and βaminoisobutyric acid from myotubes and muscle in rats and humans both exercise and nitrate stimulated pgcαmediated γaminobutyric acid gaba secretion from muscle circulating gaba concentrations were increased in exercising mice and nitratetreated rats and humans thus gaba may CF as an exercisepgcαmediated myokinelike small molecule moreover nitrate increased circulating growth hormone levels in humans and rodents nitrate induces physiological responses that mimic exercise training and may underlie the beneficial effects of this metabolite on exercise and cardiometabolic health | MEDAL |
the basolateral nucleus of the amygdala bla has been implicated in the modulation of learning after AS acute inescapable stress enhances CP eyeblink conditioning in male rats whereas the same stressor impairs eyeblink conditioning in female rats the experiments here directly assessed whether inactivation of the bla during AS SE would block both the stressinduced facilitation in males and the retardation of eyeblink conditioning in females to this end the bla was temporarily inactivated by infusion of the gaba agonist muscimol before acute stressor exposure all rats were trained in a different context h later males infused with muscimol before the stressful event did not exhibit facilitated eyeblink conditioning whereas those infused with the VH emitted more CS responses than unstressed males females infused with muscimol before stress did not express a deficit in conditioning whereas those infused with VH and stressed emitted fewer CRs than unstressed vehicle controls these data demonstrate that neuronal activity within the bla during AS SE is necessary to modulate learning h later in a new context thus the bla is necessary to induce the LT effect of stressful experience on conditioning regardless of sex and direction of modulation | MEDAL |
halogenation of bases is a widespread method used for solving crystal structures of nucleic acids however this modification may have important consequences on rna folding and thus on the success of crystallization we have used a combination of uv thermal melting steadystate fluorescence xray crystallography and gel PACE techniques to study the influence of uridine halogenation bromination or iodination on the rna folding the hiv dimerization initiation site is an rna hairpin that can adopt an alternative duplex conformation and was used as a MM we have shown that unexpectedly the rna hairpinduplex ratio is strongly dependent not only on the presence but also on the position of halogenation | MEDAL |
the aim was to test a group of aminosteroids uf ue and ue known as lazaroids for their ability to prevent alterations in neonatal rat cardiac myocytes exposed to solutions containing a xanthine oxidase mediated free radical generating system | MEDAL |
escherichia EHEC an emerging cause of foodborne disease with the occurrence of an estimated illnesses and deaths each year in the united states has now been reported from several countries worldwide infections with this bacteria which follows the ingestion of contaminated food by humans causes bloody diarrhea HUS hus and renal disease that can have serious health implications the source of food contamination is usually associated with animals mainly cattle many cattle become INF early in life when they are exposed to an environment that is contaminated by other animals shedding the organisms in their feces PCD of e coli oh in feces or contaminated food samples requires tests with high sensitivity which is increased by the use of monoclonal antibodies however the production of concentrated monoclonal antibodies in ascites raises animal welfare concerns and can be expensive in this study single chain of VL fragment scfv molecules were developed from hybridoma clones that produce immunoglobulins specific for the lps and flagella antigen of e coli oh using phage display technology the reactivity of the soluble scfv for their respective antigens was preserved in elisa and by partial inhibition of bacterial agglutination with polyclonal antiserum furthermore the scfv were able to capture e coli oh bacteria demonstrating their potential use in diagnostic assays | MEDAL |
a method is presented for efficiently computing the propagating pressure field backscattered by an arbitrarily shaped weakly scattering threedimensional object this is accomplished by drawing upon a previously reported relationship between the boundary condition on a 2DE radiating aperture and the pressure propagating along an axis normal to the aperture and the fundamental theorem of diffraction tomography which relates the fourier transform of an object CF to its scattered pressure field together these two results are used to derive an integral formula that expresses the pressure field backscattered from an object as a onedimensional fourier transform of its scattering amplitude this formula is then utilized to compute the backscattered pressure field from a uniform fluid sphere in the first born approximation the results of which are compared to the rigorous partial wave expansion | MEDAL |
the composition of bioactives including polysaccharide yield and resistant ST rs content of raw and cooked bean phaseolus vulgaris l cultivars was evaluated polysaccharide was fermented in vitro by incubation with human gut flora under anaerobic conditions and shortchain fatty acids scfas production was compared at and h by gas chromatography polysaccharide and soluble fiber contents increased upon cooking with stachyose as the L1 oligosaccharide cooked bean of cultivar bayo madero had the highest yield of PSs and resistant ST followed by those of negro and respectively acetate was the most abundant scfas formed in all bean varieties the concentration of scfas was cultivardependent bayo madero and negro displayed the highest concentration of butyrate mmoll while azufrado higuera had the lowest and highest concentrations of acetate mmoll and propionate mmoll respectively the results suggest that the common bean is an excellent source of polysaccharides that can be fermented in the colon and produce scfas compounds previously reported to exert health benefits | MEDAL |
the ultrastructure of the vasopressin neurons of the paraventricular nucleus of the hypothalamus was studied by immunocytochemical techniques tissue antigen was detected in unembedded tissue sections using a monoclonal antibody that recognizes vasopressin but not oxytocin or VT at the lightmicroscopic level RP was seen to fill the cytoplasm of the neuron cell body as well as C1 portions of the dendrite and axon immunoreactive spines were seen on both somatic and dendritic surfaces and their presence was confirmed at the ultrastructural level in the lightmicroscope axonal processes do not have spines and are thinner and more varicose than dendritic processes at the electronmicroscopic level both axons and dendrites of the vasopressin cells are filled with reactive NSG the presence of C1 numbers of these organelles made it difficult to distinguish proximal dendrites from herring bodies axonal swellings at the ultrastructural level reaction product was also observed in the cytoplasm of all segments of the vasopressin cells the presence of reaction product outside of membranous compartments is undoubtably due to disruption of CM by detergent treatment or SE to basic ph however the IF procedure used did allow us to examine the synaptic input to the vasopressin cells all portions of the vasopressin neuron receive a diverse innervation the somata have synapses on their surfaces and on spines these axosomatic terminals are primarily but not exclusively symmetrical and the presynaptic elements contain spherical or elongate LDV on the dendrites terminals again were observed on the surface or on spines these axodendritic synapses were usually asymmetrical the presynaptic elements contained clear spherical elongate or pleomorphic vesicles occasional varicosities with densecore granules were seen to make en passant contacts with dendrites these contacts did not have obvious membrane specializations input to vasopressin axons was studied both along the paraventricularneurohypophysial tract and in the median eminence vasopressin axons receive a synaptic input axoaxonic predominately of the asymmetric variety with clear spherical LDV in the presynaptic element these findings demonstrate that the vasopressin neurons of the PVH nucleus receive a diverse innervation | MEDAL |
introns are widespread and VL in eukaryotic genomes although their histories and functions or even whether all of them have any function remain largely unknown analysis of intron sequences and genomic contexts may shed CS on the evolutionary history of genes and organisms the number and frequency of introns vary widely in the small number of published genomes of protists and algae suggesting that the same is true of the vast diversity of protists and algae tl was progressively organized they improved their performance the amci group showed similar performance to the control group at the lower level of external organization but did not improve performance in conditions with a high level of external organization the mmci and vci groups showed lower performance on all tms conditions compared with controls DA revealed sensitivity and specificity to differentiate between groups based on tms conditions these results indicate how executive functions influence performance on SM tasks in elderly subjects with different neuropsychological profiles | MEDAL |
little is known about the relation of dietary AF to circulating creactive protein crp concentrations in young adults and nonwestern populations we crosssectionally examined associations between dietary NI and serum crp concentrations in young japanese women the subjects were female japanese dietetic students aged to years dietary intake was assessed with a validated selfadministered comprehensive DH questionnaire SS crp concentrations were measured by highly CS nephelometry the prevalence of elevated crp or mgl was after adjustment for possible confounding AF including body mass index a significant inverse association was seen between total n polyunsaturated fatty acid NI and elevated crp the multivariate adjusted odds ratios of elevated crp for women with intake below and above the median of SE were and confidence interval p respectively NI of eicosapentaenoic acid docosahexaenoic acid and LNA was not associated with elevated crp concentrations p and p respectively VC intake was independently inversely associated with elevated crp although the association was NS p no clear associations were observed for other dietary factors examined including total fat saturated fatty acids monounsaturated fatty acids PUFA fatty acids TDF soluble DF insoluble dietary fiber and magnesium fruits vegetables and fish and shellfish and dietary glycemic load p to p in conclusion total n PUFA fatty acid NI showed an independent inverse association with elevated serum crp concentration in a group of young japanese women | MEDAL |
the effects of addition of vitamin d and vitamin e to pig diets on BP calcium concentration meat SQ longissimus muscle and antioxidative capacity were investigated two treatments consisted of supplementation with vitamin d iud for days separately group d and a combination of vitamin e mg αtocopheryl acetatekg diet for days and vitamin d iud for days group de to growingfinishing pigs before slaughter pigs fed with vitamin d had higher p plasma calcium concentration compared with control pigs dietary supplementation of vitamin e significantly p increased the concentration of αtocopherol in meat longissimus muscle vitamin d supplementation resulted in higher p aâ values of loin chops at days of storage vitamin d and vitamin e supplementation did not affect other meat quality characteristics or tenderness quantified by warnerbratzler shear force antioxidative capacity measured as mda production T3 incubation of longissimus muscle homogenates with feascorbate was improved by VE and partly by vitamin d supplementation | MEDAL |
most dentulous unilateral cleft lip and palate patients show a tendency toward lingualized occlusion in lateral segment teeth the purpose of this T0 was to investigate the effect on the stomatognathic CF by changing the occlusal contacts of buccal cusps in lateral segment teeth in dentulous unilateral CLP patients with lingualized occlusion three patients were selected and two types of exp upper crowns l crown with occlusal contact only at the lingual cusp b crown with occlusal contacts both at lingual and buccal cusps were fabricated the border jaw movements and mandibular movements during chewing an almond were recorded with mmjie shofu inc the results were as follows by using b crown the area of border jaw movements in the frontal plane and the amount of maximum opening on the exp crowns side were increased by using b crown mandibular movements in the occlusal phase were converged and stabilized during chewing it was suggested that favorable mandibular movements during chewing were due to proper occlusal contacts provided in lateral segment teeth | MEDAL |
inhibition of binding of beta hendorphin or leuenkephalin by beta hendorphin analogs of various chain lengths in memmbrane S9 of the neuroblastoma x glioma ng cells has been investigated the removal of even a single residue from the cterminus results in the inability of the resulting peptide to completely displace beta hendorphin in addition the proportion of nondisplaceable IB increases with decreasing chain length | MEDAL |
the heparinantagonizing effect of human alpha acid glycoprotein alpha acid gp was studied by activated PTT time aptt and by a factor xa CA for heparin using a chromogenic substrate for factor xa the antiheparin effect of alpha acid ved nondialysable and resistant to heating at degrees in contrast to the MIF factor mif which could be adsorbed by macrophages eosinophil migration stimulatory activity could not be removed by preadsorption to macrophages or eosinophils | MEDAL |
rotary biventricular assist devices bivad are becoming a clinically accepted treatment option for endstage biventricular failure to improve bivad efficacy and safety we propose a control algorithm to achieve the clinical objectives of maintaining leftrightsided balance restoring physiologic flows and preventing VVI suction the control algorithm consists of two proportionalintegral pi controllers for left and right ventricular assist devices lvad and rvad to maintain differential pump pressure across lvad δpl and rvad δpr to provide leftright balance and physiologic flow to prevent ventricular suction lvad and rvad pump speed differentials δrpml δrpmr were maintained above userdefined thresholds tau and robustness of the proposed algorithm were tested in silico for axial and centrifugal flow bivad using normal and excessive δpl andor δpr setpoints rapid threefold increase in pulmonary vascular or vena caval resistances transient responses from exercise to rest and VVI fibrillation the study successfully demonstrated that the proposed bivad algorithm achieved the clinical objectives but required pressure sensors to continuously measure δpl and δpr the proposed control algorithm is device independent should not require any modifications to the pump or inflowoutflow cannulaegrafts and may be directly applied to current rotary blood pumps for biventricular support | MEDAL |
we report the cloning and characterization of a long interspersed nucleotide PE line from a cichlid fish oreochromis niloticus and show the distribution of this element called ciline for cichlid L1 in the chromosomes of this species the ID of an open reading frame in ciline with amino acid CS similarity to RTs encoded by linelike elements in caenorhabditis elegans platemys spixii schistosoma mansoni gallus gallus cri drosophila melanogaster i factor and homo sapiens line as well as the structure of the PE suggest it is a member of this family of nonlong terminal repeatcontaining retrotransposons search of a dna sequence database identified sequences similar to ciline in four other fish species haplotaxodon microlepis oreochromis mossambicus pseudotropheus zebra and fugu rubripes SBH experiments revealed the presence of sequences similar to ciline in all tilapiini species analyzed from the genera oreochromis tilapia and sarotherodon and gave an estimated copy number of about for the haploid genome of o niloticus FL in situ hybridization showed that ciline sequences were organized in small clusters dispersed over all chromosomes of o niloticus with a higher concentration near chromosome ends furthermore the long arm of chromosome was strikingly EC with this sequence the distribution of linerelated elements might underlie the difference in chromosome banding patterns observed between coldblooded vertebrates and mammals | MEDAL |
the aim of the T0 was to verify whether weeks of resistance training employing maximal isokinetic eccentric iert knee extensor actions would reduce the acute force loss observed after highintensity TM running exercise it was hypothesized that TPS iert would induce protective effects against muscle fatigue and ultrastructural damages preventing or reducing the loss in mechanical muscle CF after running subjects were tested before and after iert protocol for maximal ISO con and eccentric isokinetic knee extensor strength ° and ° s in a second session subjects performed treadmill running min and the previously mentioned measurements were repeated immediately after running subsequently subjects were randomized to training n consisting of sessions of maximal iert knee extensors actions at ° s or served as controls n the effects of acute runninginduced fatigue and training on isokinetic and isometric peak torque and rate of force OD rfd were investigated before iert runninginduced eccentric torque loss at ° s was and rfd loss was longitudinal iert led to reduced or absent acute runninginduced losses in maximal iert torque at ° s being significantly reduced compared to before iert p however rfd loss remained at p in conclusion iert yields a reduced strength loss after highintensity running workouts which may suggest a protective effect against fatigue andor morphological damages however iert may not avoid reductions in explosive muscle actions in turn this may allow more intense training sessions to be performed facilitating the AR to running training | MEDAL |
following surgery for retraction pocketcholesteatoma there is risk of RD T3 canal wall up surgery a second look tympanotomy is routinely recommended after canal wall down cwdm surgery this is not routine in certain situations the senior author recommends second look tympanotomy this report examines the outcome of this management paradigm applied to small cavity mastoid surgery for children | MEDAL |
administering a chemotherapeutic agent in combination with EFs electrochemotherapy ect has been shown to be an effective localized treatment for solid tumors the drug used most often in this combination treatment has been bleomycin ect has been used successfully in both animal studies and clinical trials the treatment was initially performed by exposing tumor cells to electrical fields following intravenous injection of the chemotherapeutic agent although ect using intravenous bleomycin was successful the procedure was limited by the existence of a narrow but optimal time window for ERP treatment as well as the fact that a systemic drug dose was being administered for a localized therapy in addition the use of i.v. also precludes the treatment of patients with poor circulation | MEDAL |
in allergic conjunctivitis the early phase reaction has been studied extensively both in humans and animals although cellular infiltration is the main feature of the late phase reaction the role of cellular immunity remains unclear the purpose of this study was to elucidate the role of cellular immunity both in the induction and effector phases of exp allergic blepharoconjunctivitis eac to analyse the involvement of cellular immunity in the induction phase weekold male lewis rats were immunized with ovalbumin ova emulsified with CFA cfa incomplete freunds adjuvant ifa titermaxr tm aluminum hydroxide aloh or without any adjuvant three weeks T3 immunization the rats were challenged with ova by eye drops and hr later they were euthanized and their eyes including the lids blood and CL nodes were harvested for analysis of disease and immune responses the results indicated that adjuvants were necessary to induce disease as well as both cellular and humoral immunity aloh cfa and tm induced stronger disease and cellular immunity than ifa the intensity of disease correlated with that of cellular immunity to further investigate the involvement of cellular immunity in eac lymph node cells collected from immunized rats were adoptively transferred into naive syngeneic recipients that were challenged days later with ova eac developed in the recipients of lymph node cells that were also stimulated in SC with ova these recipient rats developed cellular infiltration in the lid and conjunctiva in a dosedependent manner these results suggest that cellular immunity played a major role in the development of eac both in the induction and effector phases | MEDAL |
VC is associated with cardiovascular disease as a complication of hypertension hyperlipidemia diabetes mellitus and chronic kidney disease vitamin k vk delays V1 calcification by an unclear mechanism moreover apoptosis modulates vascular smooth muscle cell vsmc calcification this paper aimed to study vkmodified vsmc calcification and survival cell signaling mediated by growth arrestspecific gene gas and its tyrosine kinase receptor axl primarycultured vsmcs were dosedependently treated with vk in the presence of calcification medium for days or pretreated for h withwithout the axl inhibitor r μmoll or the caspase inhibitor zvadfmk μmoll followed by treatment with vk μmoll or rmgas nmoll in calcification medium for days calcium deposition was determined by the ocresolphthalein complexone assay and alizarin red s IF apoptosis was determined by tunel and flow cytometry using annexin vfitc and propidium iodide IF WB detected the expressions of axl gas pakt akt and bcl vk significantly inhibited cacl | MEDAL |
a model of acute suppurative cholangitis with septicemia but without shock was made in rabbits fourtyeight hours afterwards reoperation was performed with the RA major splanchnic nerve and RA celiac plexus exposed for monitoring a catheter with an inflatable rubber bag was inserted into the common bile duct via the duodenum for injection with water into the rubber bag to produce a biliary OHP of kpa which was subsequently maintained for hours the RA L1 splanchnic nerve impulse frequency was found significantly increased p the study shows that the fall of blood pressure or shock in the early stage of acute obstructive suppurative cholangitis is induced predominantly by acute biliary hypertension and our findings would be complementary to reynolds hypothesis of the production of shock | MEDAL |
cardiac surgery with cardiopulmonary bypass cpb leads to a systemic inflammatory response with secretion of cytokines eg il tnfalpha il beta and silr the objective of the following T0 was to investigate in vitro and in vivo cytokine responses and white blood cell counts wbc of patients with high versus low cytokine secretion after a CABG cabg procedure twenty male patients undergoing elective cabg surgery with cpb under GA anaesthesia were enrolled in the study on the day of surgery postoperatively SS levels of TNF and il beta were significantly higher in patients of the high il level group compared to the respective values in the patient group with low il levels the interindividual differences in il release in patients undergoing cabg surgery with cpb were accompanied by differences in the PR of other cytokines such as TNF il beta and silr to understand whether genetic background plays a role in influencing cytokine plasma levels under surgical stress we examined the distribution of polymorphic elements within the promoter regions of the tnfalpha and il genes and determined their genotype regarding the bat gene and tnfbeta intron polymorphisms our preliminary data suggests that regulatory polymorphisms in or near the tnf locus more precisely the allele set of the bat microsatellite marker combined with the g allele at of the tnfalpha gene could be one of the genetic constructions providing for a less sensitive response to various stimuli our results suggest close relationships between cytokine release in the postoperative period and interindividually varying patterns of cytokine release in patients undergoing cabg surgery with cpb | MEDAL |
k effluxed from outer hair cells and their nerves is thought to flow laterally to strial marginal cells for recycling into SM observations reported here provide evidence that k effluxed from inner hair cells and inner radial nerves travels medially through border cells inner sulcus cells iscs limbal fibrocytes and interdental cells idcs for return to endolymph morphologic features of iscs in the medial route resembled those of hensen and claudius cells in the lateral indicating an ion transport role for iscs like that of hensen and claudius cells nakatpase in plasmalemma of idcs testified to their capacity to resorb and transport k through their known gap junctions idcs were differentiated into three subgroups the most lateral idcs formed short and long columns long columns contacted the medialmost isc inferiorly and the undersurface of the TM superiorly providing thereby a potential transcellular route for k transit from iscs to endolymph short columns faced inner sulcus below and tectorial membrane above and accordingly possessed cells with opposite polarity at the bottom and top of the column short columns thus appeared situated to resorb electrolytes from limbal stroma for release into inner sulcus and beneath tectorial membrane at opposite ends of the column the CE idcs were positioned for resorbing and transporting k effluxing from the nakatpaserich stellate fibrocytes which spread toward the idcs from near the inner sulcus the most medial idcs lined cuplike invaginations near the att of reissners membrane and lay apposed to CS fibrocytes located between supralimbal fibrocytes and the medial idcs content of nakatpase and position in the k transport route likened the limbal stellate fibrocytes to the SL type ii fibrocytes and supralimbal fibrocytes to suprastrial fibrocytes in the lateral wall from content of creatine kinase and position in the transport path limbal CS fibrocytes appeared analogous to SL type i fibrocytes the additional finding that limbal fibrocytes showed unchanged or upregulated nakatpase immunoreactivity in aged gerbils with strial atrophy provided further evidence for an independent medial transport route and for the survival of inner hair cells in presbyacusis | MEDAL |
the respiratory and cardiovascular systems are highly intertwined both anatomically and physiologically respiratory and cardiovascular SN are often colocalized in the same BS regions and this is particularly evident in the ventral medulla which contains presympathetic SN in the rostral VL medulla premotor parasympathetic cardioinhibitory SN in the nucleus ambiguus and the VP respiratory group which includes the prebotzinger complex anatomical studies of respiratory and cardiovascular SN have demonstrated that many of these neurons have projections and axon collateral processes which extend into their neighboring cardiorespiratory regions providing an anatomical substrate for CR interactions as other reports in this special issue of respiratory physiology neurobiology focus on interactions between the respiratory network and baroreceptors SN in the NTS presympathetic SN and sympathetic activity this report will focus on the respiratory modulation of parasympathetic activity and the neurons that generate parasympathetic activity to the HR cardiac vagal SN | MEDAL |
bone marrow derivedmesenchymal stromal cells bmmscs are multipotent nonhematopoietic progenitors in a hematopoietic microenvironment and indispensable for regulating hematopoiesis several studies have reported that tolllike receptors tlrs are expressed in mesenchymal stromal cells mscs to modulate their biological functions in this study we investigated the possible roles of tlrs in mediating the hematopoiesissupporting role of human bmmscs human bmmscs were analyzed for mrna expression of tlr by reverse transcriptionpolymerase chain reaction tlr but not tlr were expressed by bmmscs the protein expression of tlr and tlr was also confirmed by flow cytometry we further explored the role of tlr and tlr in mediating the capacity of bmmscs to support the proliferation and differentiation of cd hematopoietic stemprogenitor cells obtained from CB bmmscs increased proliferation of cd cells and promoted the differentiation towards the myeloid lineage or days T3 coculture as well as colony formation by those cells and the production of interleukin il il il SC factor scf granulocyte colonystimulating factor csf macrophage csf and granulocytemacrophage csf if mscs had been stimulated with tlr agonist pamcsk or tlr agonist lps interestingly although these effects were elevated in a different degree a synergistic effect was not observed in bmmscs costimulated with pamcsk and lps together our findings suggest that tlr and tlr signaling may indirectly regulate hematopoiesis by modulating bmmscs functions the increased hematopoietic proliferation and differentiation could be mediated at least in part by augmented hematopoiesisrelated cytokine production of bmmscs | MEDAL |
nuance nucleus and actin connecting element was identified as a novel protein with an alphaactininlike ABD a human kb cdna of nuance spreads over kb on chromosome qq the cdna sequence predicts a kda protein with an NT actinbinding domain a CE coiledcoil rod domain and a predicted cterminal TM domain high C2 of nuance mrna were detected in the kidney CL stomach placenta spleen lymphatic nodes and IP BL at the subcellular level nuance is present predominantly at the outer nuclear membrane and in the nucleoplasm domain analysis shows that the actinbinding domain binds to factin in vitro and colocalizes with the actin cytoskeleton in vivo as a gfpfusion protein the cterminal TM domain is responsible for the targeting the NE thus nuance is the first alphaactininrelated protein that has the potential to link the microfilament system with the nucleus | MEDAL |
CCE is an often fatal disorder in the elderly clinical manifestations consist of skin lesions arterial hypertension and renal failure in some cases the clinical picture is suggestive of vasculitis the most frequent predisposing factors are operative and radiological vascular procedures and the use of anticoagulants the diagnosis can be confirmed by skin muscle or kidney biopsy data concerning management are scarce and contradictory a review of the literature has revealed some controversy as to how and when cholesterol crystal embolization should be treated and controlled studies are lacking we discuss the use of various drugs such as anticoagulants antiplatelet agents and CSs in practice the usual treatment is symptomatic and includes therapy of the PVD adequate control of blood pressure and appropriate management of renal insufficiency the most effective measure is prevention | MEDAL |
nontraumatic ABD pain is a common complaint of adult patients in acute care settings the causes of abdominal pain are numerous and can be benign or life threatening the advanced practice nurse must be able to differentiate ABD pain from acute and nonacute sources so that rapid and effective treatment can be implemented | MEDAL |
antibiotic treatment of AOM aom accounts for a significant number of all antibiotic prescriptions each year in the primary care setting initial antibiotic selection is rarely based on direct evidence such as cultures of MEF initial antibiotic therapy by the PC practitioner involves the DUE and application of information related to prevalence of infecting organisms in vitro antibiotic spectrum and penetration into middle ear fluid initial cure rate relapse and recurrence rates and antibiotic cost safety and convenience the influence of these factors on the initial antibiotic choice for aom is reviewed several therapeutic dilemmas confronting the prescriber are discussed and a rational RPA to initial antibiotic therapy is presented | MEDAL |
to compare mri findings of left ventricular apical ballooning syndrome lvabs with those of AIM ami fifteen patients with a lvabs group and patients with an ami group were explored by mri within h T3 admission comparison of both CG for the number and location of myocardial segments with abnormal wall motion and abnormal perfusion or delayed enhancement was performed the number of involved segments was higher in group than in group p in group segments with abnormal wall motion were distributed in more than one vascular territory in all patients and confined to the medial distal and apical regions of the left ventricle subendocardial hypoenhancement was observed in patients in group and in none of group p all patients in group demonstrated delayedenhancement abnormalities in a vascular CSD whereas none in group presented this abnormality p diffusely distributed segmental wallmotion abnormalities and absence of firstpass perfusion hypoenhancement and of delayed enhancement at mri help to differentiate lvabs from ami in the acute phase or in some difficult cases cardiac mri should become routine to confirm the diagnosis of lvabs | MEDAL |
with the rapid development of modern science and technology the treatment level of all disciplines of modern medicine has been rapidly improved the number of critically ill patients in large thirdlevel firstclass GA hospitals is increasing day by day intensive ECU icu has become an important embodiment of the comprehensive strength of a hospital especially the affiliated hospital of medical university or a tertiary hospital this paper aims to discuss the role status and contribution of critical care medicine in the development and construction of modern medicine and GA hospitals the relationship between hospital management and CCM management and the future OD and construction direction of critical care medicine from the perspective of the managers of thirdlevel firstclass GA hospitals | MEDAL |
to examine how the time tradeoff tto and standard gamble sg utilities perform at different lengths of life expectancy and across patient characteristics such as risk attitudes and injury severity in individuals with traumatic spinal cord injury sci | MEDAL |
our goal was to investigate whether the use of recombinant human bone morphogenetic protein rhbmp or bmp during pediatric spinal F0 surgery has been increasing and how patient surgical and hospital characteristics influence bmp use | MEDAL |
microdeletions of xp can result in contiguous gene syndromes showing the VL association of apparently unrelated clinical manifestations such as ichthyosis chondrodysplasia punctata hypogonadotropic hypogonadism anosmia ocular albinism short stature and mental retardation we report on a boy with ichthyosis dysmorphic features and mental retardation with adhd the patient was born at term T3 a pregnancy complicated by threatened abortion decreased fetal movements and low estriol serum C2 were reported during the last trimester the boy was referred to us at the age of years he presented with aggressive and hyperactive SMB he had dry hair a flat face BL lens opacities a small nose with hypoplastic tip alae nasi and nares a higharched palate with a very small cleft mixed dentition with unerupted permanent teeth left sensorineural and right mixed hearing loss with a calcified plaque of the tympanic membrane marked shortness of terminal phalanges of hands and feet ichthyosis of trunk and limbs the genomic interval between afmth and kal was investigated pcr analysis showed a deletion in xp with the distal breakpoint between the marker afmth and pabx and the PT one between dxs and kal arraycgh and fish analysis confirmed the interstitial deletion of about mb and refined the breakpoints we discuss the phenotype of our patient in relationship to the deleted segment and the possibility of mental retardation and adhd genes in the region | MEDAL |
the recent discovery of ammoniaoxidizing archaea aoa dramatically changed our perception of the diversity and evolutionary PH of microbes involved in nitrification in this T0 a moderately thermophilic degrees c ammoniaoxidizing enrichment culture which had been seeded with biomass from a hot spring was screened for ammonia oxidizers although gene sequences for crenarchaeotal s rrna and two subunits of the ammonia monooxygenase amoa and amob were detected via pcr no hints for known ammoniaoxidizing bacteria were obtained comparative CS analyses of these gene fragments demonstrated the presence of a single operational taxonomic unit and thus enabled the assignment of the amoa and amob sequences to the respective s rrna phylotype which belongs to the widely distributed group ib soil group of the crenarchaeota CARD cardfish combined with microautoradiography mar demonstrated metabolic activity of this archaeon in the presence of ammonium this finding was corroborated by the detection of amoa gene transcripts in the enrichment cardfishmar showed that the moderately thermophilic aoa is highly active at and mm ammonium and is partially inhibited by a concentration of mm the enriched aoa which is provisionally classified as candidatus nitrososphaera gargensis is the first described thermophilic ammonia oxidizer and the first member of the crenarchaeotal group ib for which ammonium oxidation has been verified on a cellular level its preference for thermophilic conditions reinvigorates the debate on the thermophilic ancestry of aoa | MEDAL |
in this study an isothermal microcalorimetric technique was used to demonstrate the impact of DMP dmp dinoctyl phthalate dop and diethyl phthalate dep on soil microbial activity the effect of these PEs ⤠μg gâ»Â¹ soil follows the order dep dmp dop but changed to dmp dep dop when the concentrations of the phthalate esters are above μg gâ»Â¹ soil dmp dep and dop significantly decreased p the soil urease activity the effect of PEs on soil microbial activity is not the same with those on enzymatic activity in addition dep which has a lower bioavailability is less toxic to soil microbes than that of dmp when its concentration is above μg gâ»Â¹ soil | MEDAL |
we developed a linear CAS qol scale to measure the quality of life qol in elderly patients with chronic lung disease in this T0 the validity of the qol scale was assessed and qol scale scores were compared with the results of other conventional questionnaires a total of subjects aged years or older divided into three groups according to disease severity were tested by the qol scale and two additional questionnaires the qol scale had the advantages of comprehensibility acceptability and reproducibility qol scale scores differed among the CG while the other questionnaires showed no significant differences according to disease severity qol scale scores correlated with the tendency toward neurosis shown by another index we conclude that the qol scale is a practical and useful indicator of qol in elderly patients with CLD | MEDAL |
we report the case of an year old man who developed a small diffuse lymphoplasmacytic lymphoma that involved the entire gastrointestinal tract the malignant lymphoma was associated with intestinal amyloidosis presumably produced by the neoplastic lymphocytes the outstanding symptoms were asthenia fever marked weight loss diarrhea and melena the postmortem study disclosed in addition lymphomatous infiltration to the GB mesenteric CL nodes and testis the case represents an exceptionally widespread type of diffuse gastrointestinal lymphoma | MEDAL |
the efficacy of anaesthetic premedication has been assessed using sedative scores or a visual analogue scale however in both it may be difficult to exclude evaluators subjectivity or a placebo effect plasma concentration of catecholamines may also be useful for the assessment of patient anxiety recently BIS electroencephalographic analysis has been developed and the bispectral index monitor has been reported to give measurements which correlate well with the depth of sedation in the present study we have examined the relation between BIS values and plasma catecholamine concentrations after oral diazepam premedication twentyeight patients scheduled for elective surgery were randomly assigned to one of two CG diazepam premedication group group d n and no premedication group group d n the patients were premedicated orally with diazepam mg and roxatidine mg in group d and with roxatidine mg only in group d min before arrival in the operating theatre after patients arrived in the OT the BIS monitor was applied venous blood samples ml were collected in the case of patients in group d for the measurement of plasma catecholamines levels using highperformance liquid chromatography the BIS level mean sd in group d was significantly lower than that in group d p there was a significant rho between BIS and plasma norepinephrine C2 r p the present T0 suggests that the bispectral index monitor may detect the effect of oral diazepam premedication | MEDAL |
recombinant bacterial plasmids that contain dna cDNA to human PPI messenger rna have been constructed one clone contains the entire PPI coding region as well as the untranslated region of the messenger rna and eight nucleotides of the untranslated region additional CS information for the untranslated region was obtained with the use of insulinoma messenger rna in conjunction with TPS primers from the cloned dna for enzymatic chain termination sequence analysis the results confirm the amino acid CS of human PI previously determined and predict the amino acid CS of the human preproinsulin signal peptide | MEDAL |
the biological and physiological effects of exogenous fgf and vegf were measured using the kht mu fibrosarcoma RT MM tumorbearing ch mice were treated intratumorally with either one or six daily doses of microgmouse fgf vegf or saline PT were excised hrs after the final i.t. compared to controls only fgf treatment significantly increased tumor weight and size and only in the dose group both fgf and vegf administration dose decreased tumor cell hypoxia as detected by ef uptake for fgf and for vegf versus for controls decreased tumor cell ef IF however was not associated with changes in numbers of structural or angiogenic vessels dioc staining showed a slight decrease in perfused vessel numbers in PT treated with daily vegf intratumoral injections of fgf or vegf also slightly decreased the RT tissue chemokine mcp interleukins ilbeta il and il mrna expression and increased nfkappab binding without altering ap binding of ikappab protein expression in summary single pulse exposures of tumors to angiogenic factors had little or no effects on tumor growth or perfusion while daily exposures stimulated tumor growth through improved RT oxygenation this improved V1 CF occurs without an increase in vascular density | MEDAL |
vasovagal reflexes such as hypotension and bradycardia are induced by rapid hemorrhage and mimic neurocardiogenic reflexes in mammals we examined the role of vasopressin in the neurocardiogenic responses to mild rapid hemorrhage ml g for seconds and severe hemorrhage ml g body wt for seconds repeated three times at minute intervals in homozygous brattleboro and LE mild rapid hemorrhage induced severe bradycardia and SH only in LE exogenous vasopressin pmolkg per minute for hour restored both the bradycardic and hypotensive responses in brattleboro rats ddavp a vasopressin vreceptor agonist pmolkg per minute for hours did not affect the cardiovascular responses to hemorrhage in brattleboro rats although it maintained urine production within normal limits however opc mumolkg iv a vasopressin vreceptor antagonist attenuated both the hypotensive and bradycardic responses to hemorrhage in LE a vasopressin vreceptor antagonist att bradycardia and delayed the REC of arterial pressure after hemorrhage but did not affect the hypotension that occurred immediately T3 hemorrhage in LE methylatropine also att both the bradycardic and hypotensive responses induced by hemorrhage but propranolol had no effect on the cardiovascular responses to hemorrhage in longevans rats the recovery of arterial pressure T3 repeated hemorrhage was less adequate in DI than in longevans rats our results suggest that the neurocardiogenic responses to hemorrhage especially SH may be related to vasodilation induced by a vreceptormediated mechanism and by the vagal reflex both of which are substantiated by the existence of vasopressin the coexistence of v and vreceptor mechanisms may be necessary for the hypotensive response to hemorrhage we found that a vreceptor antagonist attenuated the hypotension mediated by the socalled neurocardiogenic reflex | MEDAL |
as ultrasound technology advances diagnosis of fetal malformations particularly congenital heart defects chd is becoming standard practice currently a key element of obstetrical care is the use of ultrasound to diagnose chorionicity in multiple gestations given the difference in incidence and types of complications between DC and monochorionic pregnancies early diagnosis of chorionicity is critical to determine the type of care and counseling a patient receives throughout the pregnancy early diagnosis of chorionicity allows investigators to more accurately determine the risk of chd in MC pregnancies it has been long known that twin gestations incur a higher risk of congenital malformations including chd however it was not until recently that the incidence could be determined according to chorionicity previous studies looking at risk of malformations including chd used the likesex technique as a proxy for chorionicity thereby overestimating the prevalence of MC twins because roughly twothirds of all twin gestations including dichorionic are the same sex the rate of multiple gestations is increasing in the developed world assisted reproductive technology art is partly responsible for the increased incidence of multiples while many of the art conceived pregnancies are dichorionic multiples there is evidence that art increases the risk of MC multiple gestations presently it is not technically feasible nor practical to screen all pregnancieson combined with singleshot capability offers great advantage for flash imaging of delicate samples | MEDAL |
the coding CS for flp recombinase originally from the mu plasmid of saccharomyces cerevisiae was introduced into arabidopsis behind the cauliflower PMV s promoter flp activity was monitored by the glucuronidase activity resulting from inversion of an antisenseoriented gus reporter gene flanked by a pair of frt target CS in inverted repeat flpdependent gus activity was observed in both transient assays and transgenic plants the flp system will be useful for a variety of in planta genetic manipulations | MEDAL |
to investigate the racial heritage of a group of africanamericans who exhibit the pigment dispersion syndrome pds | MEDAL |
expression of the retinoblastoma rb tumor suppressor gene during cell differentiation induced by dimethyl sulfoxide or sodium butyrate was studied in hl human promyelocytic one cells as cells progressed through the Tc the amount of rb protein per cell increased with homeostasis maintained so that the amount of rb protein relative to the total cell mass remained almost constant dimethyl sulfoxide was used to induce these PML cells to undergo terminal differentiation into mature myeloid cells there was an early reduction in the rb protein expressed per cell the reduction in expression was similar for cells in all Tc phases there was also progressively reduced expression at later times as cells terminally differentiated this was compared to the case in which sodium butyrate was used to induce the differentiation of hl cells into mature monocytic cells an early reduction in rb protein expression per cell also occurred it occurred for cells in all cell cycle phases as well thus the induced differentiation of hl cells along either the myeloid or the monocytic differentiation lineage involves an early reduction in rb expression which is common to both pathways the reduction anteceded proliferative arrest or differentiation in both cases the final resulting gdifferentiated cells had less rb protein per cell than the proliferating immature leukemic F0 cells | MEDAL |
an enzymelinked immunosorbent assay and western immunoblotting analysis were used to detect antiplatelet antibodies in the sera of patients with SVR sclerosis eleven positive sera were found by elisa serial investigations showed that the presence of these antibodies was often transient immunoblotting analysis demonstrated that the antibodies were directed against a kda antigen using monoclonal antibody antiplatelet antibodies directed against gpiiia were found in cases similar to immune thrombocytopenia and lupus erythematosus a significant portion of the antiplatelet antibodies were also directed against the glycoprotein complex iibiiia in systemic sclerosis | MEDAL |
twentysix patients underwent resection and VG replacement of an aortic arch aneurysm proximal arch transverse arch distal arch and type a dissecting aneurysm retrograde rCBF with pharmacological cerebral protection was carried out during aortic arch aneurysm surgery prostaglandin e thiopental methylpredonisolone were po for CBF protection during core cooling dmannitol and deferoxamine mesylate radical scavengers were po for prevention of reperfusion injury RCP time was T2 range T2 perfusion flow was mlmin range mlmin since VA rCBF requires no arterial cannulation or aortic cross clamp the operative field is simplified and the risks of air and debris emboli to the BB were minimized reconstruction was designed to minimize the circulatory arrest time eleven cases underwent emergency surgery due to rupture and acute dissection five patients died three from bleeding from the distal anastomosis one from postoperative dic and one from intraoperative dissection the remaining patients survived neurologically IN RCP with pharmacological cerebral protection is a very simple method to prevent PAE or TE in aortic arch aneurysm surgery and allows aortic arch replacement in a bloodless field in spite of the D2 circulatory arrest time REC of consciousness was CR | MEDAL |
a previously healthy yearold girl suffered from oral herpetic gingivostomatitis after four days oral administration of acyclovir acv mg in five divided doses was started she became irritable and intermittently delirious next day laboratory tests of blood and CSF fluid csf were within normal limits and eeg showed no abnormality BB ct and mri demonstrated mild asymmetry of the lateral IVT spect showed low perfusion area in the BL TH the titer of igg and igm against herpes simplex virus hsv was increased in the SS but not in the csf a pcr study of hsv dna was negative in the csf acv was discontinued T3 days administration and she recovered from neurological disorders within hours acv neurotoxicity is selflimiting and dosedependent it may be distinguished from herpes simplex encephalitis by the lack of fever HA focal neurologic signs and abnormal csf and neuroradiological findings however when the differentiation should be difficult we believe that the administration of acv can be carefully continued because the potential fatality of hse is high while acv neurotoxicity is reversible | MEDAL |
to gain an insight in the regulation of rhydroxycalcidiol we studied the pharmacokinetics of orally administered rhydroxycalcidiol in healthy subjects without calcium supplementation in healthy subjects with calcium supplementation and in patients with primary hyperparathyroidism various quantities related to calcium and vitamin d metabolism were also monitory blood supply and its influence on outcome ductal supply of confluent pulmonary arteries was present in patients group i whereas patients group ii had a pulmonary blood supply that was entirely or predominantly dependent on SVR collateral arteries over the years there was no significant difference in AS between the two CG corrective surgery was performed in of patients in GI significantly more than in group ii of arborization abnormalities of the pulmonary arteries stenosis of unbranched and intrapulmonary arteries were almost exclusively present in patients with SVR collateral arteries p accounting for the lower probability of undergoing corrective surgery in group ii patients during the first decade of this T0 corrective surgery was attempted in of patients with mortality and during the second decade surgery was performed in of patients with mortality a significantly lower figure improving surgical results CR preoperative demarcation of the pulmonary blood supply and a more aggressive approach with early unifocalization of the pulmonary blood supply may invalidate comparison with retrospective data on the advisability of attempting to correct this anomaly the present paper provides data against which treatment of infants with PA and ventricular septal defect presenting during the next decade can be compared | MEDAL |
the transcriptome analysis of wholeblood rna by sequencing holds promise for the ID and tracking of biomarkers however the high globin mrna gmrna content of erythrocytes hampers wholeblood and buffy coat analyses we introduce a novel gmrna locking assay globinlock gl as a robust and simple gmrna reduction tool to preserve rna quality save time and cost gl consists of a pair of gmrnaspecific oligonucleotides in rna initial denaturation buffer that is effective immediately T3 rna denaturation and adds only ten T2 of incubation to the whole cdna synthesis procedure when compared to nonblood rna analysis we show that gl is fully effective not only for human samples but also for mouse and rat and so far incompletely studatively largescale receiver array in a dynamic caribbean coastal environment intended for longterm monitoring of multiple species the us geological survey and several academic institutions in collaboration with CRM at buck island reef national monument birnm off the coast of st croix recently deployed a passive acoustic receiver array we targeted arrayrepresentative receivers for rangetesting by submersing fixed delay interval rangetesting tags at various distance intervals in each cardinal direction from a receiver for a minimum of an hour using a generalized linear mixed MM glmm we estimated the probability of detection across the array and assessed the effect of water depth habitat wind temperature and time of day on the probability of detection the predicted probability of detection across the entire array at m distance from a receiver was ci and dropped to ci m from a receiver indicating a somewhat constrained ERP PCD range PCD probability varied across habitat classes with the greatest ERP detection range occurring in homogenous sand substrate and the smallest in high rugosity reef predicted probability of PCD across birnm highlights potential gaps in coverage using the current array as well as limitations of passive acoustic technology within a complex coral reef environment | MEDAL |
the susceptibilities of clinical isolates of various aspergillus species aspergillus fumigatus a flavus a terreus a ustus and a nidulans to itraconazole itc and amphotericin b amb were determined using the standard proposed by nccls for antifungal susceptibility testing offilamentous fungi a modification of this method using spectrophotometric readings and a colorimetric method using the tetrazolium salt bis methoxynitrosulfenylamino carbonylhtetrazoliumhydroxide xtt five mic end points for itc mic no visible growth or or the growth control value gc mic slight growth or to the gc mic prominent reduction in growth or to the gc mic slight reduction in growth or to the gc and mic no reduction in growth or to the gc and one for amb mic were determined visually by four observers and spectrophotometrically the intraexperimental between the observers and interexperimental between the experiments C2 of PA of the nccls and xtt methods exceeded for mic of amb and mic and mic of itc the mic of itc showed lower reproducibility although spectrophotometric reading andor incubation for h increased the interexperimental reproducibility from to between visual and spectrophotometric readings high C2 of PA were found for amb approximately and mic approximately and mic approximately of itc poor PA was found for mic of itc T3 h since the spectrophotometric readings resulted in higher mic values than the visual readings the agreement was increased to by shifting the threshold level of mic from to ROD and by establishing an optical density of greater than for the gc as the validation criterion no statistically significant differences were found between the nccls method and the xtt method with the C2 of agreement exceeding for mic of amb and for mic mic and mic of itc the xtt method and spectrophotometric readings can increase the sensitivity and the precision respectively of in vitro susceptibility testing of aspergillus species | MEDAL |
chemical analysis of the ultravioletabsorbing materials in american medical optics cilco and optical radiation corporation intraocular lenses is reported clinical considerations are discussed and the absence of any LT safety and effectiveness data is noted | MEDAL |
in systematic reviews and metaanalyses timetoevent outcomes are most appropriately analysed using hazard ratios hrs in the absence of individual patient data ipd methods are available to obtain hrs andor associated statistics by carefully manipulating published or other summary data awareness and adoption of these methods is somewhat limited perhaps because they are published in the statistical literature using statistical notation | MEDAL |
the effects of the beta adrenergic agonist hexoprenaline were studied on the progesterone production of rat and human corpora lutea and compared to hcginduced hormone production human corpora lutea were obtained from healthy patients rat corpora lutea were harvested on day of pseudopregnancy corpora lutea were digested by trypsin and homogeneous luteal cell suspension x cellsml was incubated for h hexoprenaline and hcg were added to the medium and progesterone production was measured by ria hexoprenaline or hcg dosedependently increased the progesterone production of rat luteal cells and of human cells in mid and late LP moreover hexoprenaline further increased the hcginduced hormone production the stimulatory effect of hexoprenaline could be prevented by propranolol it is supposed that beta adrenergic stimulation induces an increase in progesterone production of luteal cells and potentiates the effects of gonadotropic hormones | MEDAL |
the secreted glycoside hydrolase family gh αlfucosidase from plant pathogenic fungus fusarium graminearum fgfco actively releases fucose from the XG fragment we solved crystal structures of two activesite conformations ie open and closed of apofgfco and an open complex with product fucose at atomic resolution the closed conformation supports cataage range of routine vaccination will be required measles vaccine coverage remains far below targets for herd immunity in many countries on the african continent and is likely to be inadequate for achieving rubella elimination the impact of strategies such as increasing the upper age range eligible for routine vaccination should be considered | MEDAL |
pyogenic sacroiliitis is a rare entity in children diagnosis is often delayed because of its variable clinical presentation low suspicion by the examining physician and rare findings on radiographs delayed diagnosis however results in complications such as iliopsoas abscess we report the case of a yearold girl hospitalized with a day PH of fever pain in the left iliac fossa and flexion contracture of the hip on examination she had fever degrees c psoitis localized tenderness at the left sacroiliac joint and pain elicited by lateral compression of the pelvis the ABD examination was normal the erythrocyte sedimentation rate was mm in the first hour creactive protein was mgl and the WG blood cell count was mm with neutrophils urinalysis was normal blood cultures were negative radiographs of the pelvis showed irregular left SI borders the ct scan provided the diagnosis of sacroiliitis complicated by an ilioapsoas abcsess treatment was based on antibiotic therapy associated with surgical drainage bacteriologic investigation revealed staphylococcus aureus the patients temperature returned to normal on the second day antibiotics were continued for months leading to full recovery | MEDAL |
the extent to which high muscle tone contributes to the caloric needs of children with CP cp is debated understanding its contribution is important in predicting calorie needs in this population which frequently experiences under nutrition ITB is an innovative therapy now used for treatment of excessive tone we prospectively studied the changes in resting energy expenditure ree observed in children with cp undergoing ITB injection ree was measured in the fasting state before intrathecal injection of micrograms of baclofen patients remained fasting and ree measures were repeated four hours later tone changes were assessed using the modified ashworth scale at the same time points one child was excluded from T0 because he became increasingly agitated over the observation period and one child demonstrated no tone reduction T3 the baclofen i.t. in the remaining patients the average ree prior to i.t. was ± of predicted and decreased to ± of predicted after injection p this study suggests that tone contributes substantially to the caloric needs of children with cp and must be carefully considered when providing nutritional recommendations | MEDAL |
the aim of the study was to analyze the results of subtalar stabilization of the foot by conventional open surgery with the use of bicortical graft in CP patients with pes equinovalgus and to compare our results with those of other authors | MEDAL |
semliki forest virus sfv sindbis virus sin and venezuelan equine encephalitis virus are simple enveloped plusstrand rna viruses belonging to the alphavirus genus of the togaviridae family they have been developed into expression vectors that infect a wide host cell range and cause rapid and highlevel transgene expression their easy and fast generation classification into biosafety levels and and preferential transduction of SN in cell and tissue cultures makes them an increasingly used gene transfer system this review summarizes the alphaviral replication and expression the replicon system and its application in neurobiology alphaviral vectors can introduce multiple transgenes into host cells and mutants with low or absent cytotoxicity and increased or decreased transgene expression C2 are available temperaturedependent mutants permit to control the host cell specificity as well as the on and offset of gene expression these features together with the transduction characteristics revealed in a direct comparison of alphaviral and other viral vectors in hippocampal slice cultures make sfv and sin vectors a powerful tool for neurobiological studies | MEDAL |
in an attempt to establish a colony of atopic dogs as a research resource dogs with seasonal dermatitis a reported manifestation of naturally occurring allergy in that species were identified acquired and inbred progeny were studied during their first years of life to determine if the trait could be genetically transmitted and to investigate the hypothesis that this spontaneously occurring dermatitis correlates with positive laboratory measurements for TPS ige to airborne allergens serial immunoglobulin levels including total ige endpoint titration of histamine and results of fecal examinations for ID of parasites were also investigated and are to be the subject of a separate publication only a weak correlation between positive direct skin test results and the appearance of seasonal dermatitis was found in initial studies neither the results of testing by PCA nor radioallergosorbent procedures correlated with positive clinical findings independently conducted clinical examinations and direct ST using selected antigens as well as additional bronchial challenges of representative animals from the colony also failed to furnish evidence of atopy in the dogs therefore the goal of establishing an inbred line of atopic animals was not accomplished within the allotted year period also the data did not show a positive correlation between the seasonal dermatitis and measurements for specific ige to airborne allergens | MEDAL |
very potent inhibitors were synthesized for the enzymatic deacetylation of nacetyldglucosaminephosphate naga and nacetyldglutamate dgd the methyl phosphonamidate derivative of dglucosaminephosphate bound to nacetyldglucosaminephosphate deacetylase with an equilibrium dissociation constant of nm at ph and an association rate constant of x m s the inhibition constant is fold lower than the K(m) for the ATP nacetyldglucosaminephosphate nacetyldglutamate deacetylase was inhibited by the methyl phosphonamidate derivative of dglutamate with an inhibition constant of pm at ph the inhibitor bound to the enzyme fold tighter than the michaelis constant for nformyldglutamate these EDC mimic the putative tetrahedral intermediate formed upon nucleophilic attack of an G1 water molecule on the amide bond of the target ATP these inhibitors should prove useful in the elucidation of the ES interactions for enzymes within the amidohydrolase superfamily | MEDAL |
little is known about the nature of the calcium channels controlling neurotransmitter release from preganglionic parasympathetic nerve fibres in the present T0 the effects of selective calcium channel antagonists and amiloride were investigated on ganglionic neurotransmission conventional intracellular recording and focal extracellular recording techniques were used in rat submandibular and pelvic ganglia respectively excitatory postsynaptic potentials and excitatory PSCs preceded by nerve terminal impulses were recorded as a measure of acetylcholine release from parasympathetic and sympathetic preganglionic fibres following nerve stimulation the calcium channel antagonists omegaconotoxin gvia n type nifedipine and nimodipine l type CTX mviic and omegaagatoxin iva pq type and ni r type had no PET inhibitory effects on synaptic transmission in both SM and pelvic ganglia the potassiumsparing diuretic amiloride and its analogue dimethyl amiloride produced a reversible and concentrationdependent inhibition of excitatory postsynaptic potential amplitude in the rat SMG the amplitude and frequency of spontaneous excitatory postsynaptic potentials and the sensitivity of the postsynaptic membrane to acetylcholine were unaffected by amiloride in the rat pelvic ganglion amiloride produced a concentrationdependent inhibition of excitatory PSCs without causing any detectable effects on the amplitude or configuration of the nerve terminal impulse these results indicate that neurotransmitter PR from preganglionic parasympathetic and sympathetic nerve terminals is resistant to inhibition by specific calcium channel antagonists of n l pq and rtype calcium channels amiloride acts presynaptically to inhibit evoked transmitter release but does not prevent action potential propagation in the nerve terminals suggesting that amiloride may block the pharmacologically distinct calcium channel types on rat preganglionic nerve terminals | MEDAL |
adrenocorticotropin IB sites in the rat ME have been localized in vivo these binding sites occur in the basalar zone which is rich in axonal endings using competitive binding and quantitative lightmicroscope radioautography we found that the medianeminence binding site in contradistinction to the adrenal receptor binds specifically the residue region of the ACTH molecule using quantitative electronmicroscope radioautography and medianeminence deafferentation we localized the binding sites to axon terminals in this region in timedelayed uptake studies using lightmicroscope radioautography we failed to observe concentration of radiolabel in neurons of the medial basal hypothalamus T3 the direct i.t. of radioiodinated adrenocorticotropin into the median emined differentiation with minimal loss of cell viability continuous exposure of felc from h with doses of aphidicolin ranging from to microm was more effective for differentiation induction than was ST SE h to the drug although h of exposure significantly p less than increased differentiation compared with UT cells when cells were treated with hmba mm and aphidicolin microm in combination aphidicolin shifted the time of ON of differentiation from to h but did not act synergistically or additively with hmba nor was the induction effect of aphidicolin changed by hmba in contrast suboptimal doses of aphidicolin microm in combination with hmba mm produced an additive effect on felc differentiation in addition hthymidine experiments demonstrated that aphidicolin reversibly blocked felc in s phase and at gs interface of the cell cycle these results indicate that aphidicolin can induce the differentiation of felc and that a CR round of replicative dna synthesis is not required for differentiation to occur | MEDAL |
this study is the first costbenefit analysis cba of OHS ohs in a lowincome country it focuses on one of the largest shipbuilding companies in bangladesh where globally recognised occupational health and safety advisory services ohsas certification was achieved in | MEDAL |
this study investigates the probable causes of nerve root palsy through the retrospective study of pre and postoperative C2 curvature change for patients with CS and incidences of nerve root palsy a consecutive series of patients with C2 compressive myelopathy treated by laminectomy and internal fixation were reviewed nerve root palsy developed in of patients group a the other patients men and women were chosen as controls group b a neutral lateral cervical spine radiograph was taken of all patients the overall curvature of the cervical spine the cervical curvature index and the NC rate were measured and comparedthe pre and postoperative NC rate of cervical curvatures in groups a and b was and respectively the difference was not statistically significant p the pre and postoperative C2 curvature index change rate in groups a and b was and respectively there were statistically significant differences betwerse isolates closely related isolates and isolates from seven outbreaks the evaluations revealed that five of the vntrs had diversity values greater than and three sttr sttr and sttr were hypervariable the results obtained from the outbreak isolates suggested that the vntrs were considerably stable in isolates recovered during a normal outbreak time course multilocus vntr analysis mlva based on four most VL vntrs mlva exhibited a better resolving SP over pulsedfield gel PACE pfge in discriminating among isolates in particular among the closelyrelated isolates an mlva which is based on five vntrs and has been widely used in many european laboratories displayed a level of discrimination close to mlva the phylogenetic tree established using the mlva profiles presented four distinct clusters which were associated with four different phage types therefore mlva based on four or five highly VL vntrs is sufficiently powerful to supplement or replace pfge for outbreak investigation and surveillance of s typhimurium infections and mlva data based on vntrs can be useful in establishing clonal structures among isolates | MEDAL |
to describe the clinical ophthalmological endocrinological and radiological features of saudi children with the syndrome of septooptic dysplasia and Thy hypopituitarism | MEDAL |
early RC for a highgrade RT invading the lamina propria t remains controversial in we cannot identify accurately which of these highrisk PT will progress to muscleinvasive disease and metastases in the near future urologists may be able to use the presence of genetic alterations such as p mutations to help make therapeutic decisions previous reports on SF bladder CA treated with intravesical bacillus calmetteguérin immunotherapy have demonstrated a decrease in recurrence and progression unfortunately there is no reliable method to predict which patients with a highgrade t tumor will fail to respond to intravesical therapy failure of intravesical therapy to control these aggressive tumors is associated with a significant rate of pathological upstaging and metastases RC will cure a high percentage of these t PT with acceptable morbidity and low mortality in an era of nervesparing cystectomy and orthotopic neobladder reconstruction early radical cystectomy is an alternative that should be discussed with the patient before instituting intravesical therapy | MEDAL |
previous studies of patients with thromboembolic disease have revealed an association either with hereditary AC protein deficiencies or with defects in the fibrinolytic system to obtain a more comprehensive picture and to investigate which analyses are useful in the evaluation of such patients we have performed an extensive laboratory investigation in individuals with TE disease AC protein deficiencies were found in patients deficiencies of protein c n and protein s n were most common followed by deficiencies of AT iii n and plasminogen n six of the nine protein s deficient patients demonstrated a selective deficiency of free protein s with normal total protein s concentrations to diagnose protein c and s deficiencies among the patients receiving oral vitamin k antagonists the concentrations of protein c and s were compared with the mean concentration of several other vitamin kdependent proteins one protein c and three protein s deficiencies were identified among the treated patients the number of protein c deficiencies found in this group was significantly lower than the number found among UT patients although fewer protein s deficiencies were also identified among the treated patients than in the untreated group the difference was not statistically significant the results suggest that protein c deficiencies went undetected in the treated group and that OAT should be discontinued before efforts to diagnose protein c deficiency are made we found no cases with heparin cofactor ii deficiency lupus anticoagulant was present in patientsabstract truncated at words | MEDAL |
neuropeptide fflike immunoreactive npffir cells and fibers were analyzed through development of xenopus laevis the first npffir cells appeared in the embryonic hypothalamus which projected to the intermediate lobe of the hypophysis the brainstem and spinal cord slightly later scattered npffir cells were present in the OBs and VP TEL in the caudal medulla npffir cells were observed in the nucleus of the solitary tract only at embryonic and early larval stages abundant npffir cells and fibers were demonstrated in the spinal cord the CS of appearance observed in xenopus shares many developmental features with mammals although notable differences were observed in the TEL and hypothalamus in general npff immunoreactivity developed earlier in amphibians than in mammals | MEDAL |
hour infusion of iloprost was compared with PL infusion in patients with SD intermittent claudication using a double blind balanced crossover design despite significant inhibition of PLT aggregation to adp and collagen p less than and the typical cardiovascular and gastrointestinal SE there was no significant effect on treadmill exercise times at any time up to weeks T3 infusion the confidence limits indicated that an improvement of more than was unlikely to occur no significant changes in b thromboglobulin platelet aggregate ratio BT whole BV and euglobulin clot lysis time were demonstrated | MEDAL |
crusted scabies norwegian scabies is uncommon in clinical practice and may present as papulosquamous dermatosis a case of crusted scabies in SLE erythematosus sle is described the skin lesions simulated those of subacute cutaneous lupus erythematosus of the papulosquamous type and became the source of epidemic scabies in the hospital the colonization with staphylococcus aureus within crusted lesions may have contributed to the patients sepsis this case illustrates the pitfalls in recognition of crusted scabies and the importance of rapid diagnosis | MEDAL |
iodide concentration by the thyroid gland an essential step for thyroid hormone synthesis is mediated by the NIS nis to identify AF that may regulate this process we have studied nis mRNA in the fisher rat thyroid cell line frtl by a semiquantitative reverse transcriptionpolymerase chain reaction rtpcr technique increasing concentrations of bovine tsh and mul with or without tumour CN factoralpha tnf alpha interferongamma ifn gamma or interleukin alpha il alpha were added to frtl cells previously deprived of tsh for a minimum of days rna was extracted and samples were studied for nis expression tsh enhanced nis mrna expression in a dosedependent manner with induction evident at mul reaching a peak at mul an effect detected T3 h of stimulation but not in the first h both tnf alpha and to a lesser extent il alpha inhibited basal and tshinduced nis expression high concentrations of ifn gamma also downregulated tshstimulated nis mrna expression using the same technique we also investigated nis mrna tissue CSD in two male and one female wistar rats high levels of nis expression were detected in the thyroid stomach and MG lower levels were found in the intestine adipose tissue and CL BL C2 were expressed in the SSG and no expression was detected in the kidneys in summary we have shown that tsh upregulates rat nis gene expression in vitro and this induction can be modulated by cytokines analysis of the CSD of rat nis mrna ex vivo demonstrated variable levels of nis transcription in different tissue samples | MEDAL |
we T0 the load distribution in weighted networks by measuring the ERP number of optimal paths passing through a given vertex the optimal path along which the total cost is minimum crucially depends on the cost distribution function pc c in the strong disorder limit where pc c approximately c the load distribution follows a SP law both in the erdösrényi er random graphs and in the scalefree sf networks and its characteristics are determined by the structure of the minimum spanning tree the CSD of loads at vertices with a given vertex degree also follows the sf nature similar to the whole load CSD implying that the global transport property is not correlated to the local structural information finally we measure the effect of disorder by the rho coefficient between vertex degree and load finding that it is larger for er networks than for sf networks | MEDAL |
the objectives of our T0 were to describe the previously unreported normal mr anatomy of the distal biceps femoris muscle and its relationship with the common peroneal nerve anfor different alleles testing of different modes of inheritance and heterogeneity of risk between alleles it is applicable to risk alleles for any disease locus or incompletely penetrant phenotype we have used the method to estimate risks of crohn disease for different cardnod mutations using the families originally collected to identify this susceptibility locus the odds ratio of crohn disease were respectively and for the rw gr and fs heterozygotes and and for the corresponding homozygotes signifies insufficient data to estimate these values these results confirm the dosage effect for cardnod mutations and demonstrate that the disease risks are very different in homozygotes this last observation illustrates the SP of this RPA especially for alleles with low or moderate frequency in the GA population | MEDAL |
in this paper we consider a deterministic malaria transmission MM with standard incidence rate and treatment human population is divided into susceptible infectious and recovered subclasses and mosquito population is split into susceptible and infectious classes it is assumed that among individuals with malaria who are treated or recovered spontaneously a proportion moves to the recovered class with temporary immunity and the other proportion returns to the susceptible class firstly it is shown that two endemic equilibria may exist when the basic reproduction number r and a unique endemic equilibrium exists if r the presence of a backward bifurcation implies that it is possible for malaria to persist even if r secondly using geometric method some sufficient conditions for global stability of the unique endemic equilibrium are obtained when r to deal with this problem the estimate of the lozinskiÄ±Ë measure of a à matrix is discussed finally numerical simulations are provided to support our theoretical results the model is also used to simulate the human malaria data reported by the chinese ministry of health from to it is estimated that the basic reproduction number râ for the malaria transmission in china and it is found that the plan of eliminating malaria in china is practical under the current control strategies | MEDAL |
to evaluate the tau and to investigate the association between the length of the treatment period and the cumulative dose of PEG interferon alphaa pegifn alphaa plus ribavirin rbv and the effectiveness of antiviral therapy we analyzed data from patients treated for weeks with pegifn alphaa mug or mugweek plus weightbased rbv mgd for patients is less than or equal to kg mgd for patients kg and mgd for patients is more than or equal to kg under care at west china hospital hcv rna was assessed at baseline week and the end of treatment eot and after weeks followup SVR svr with a test range of to iuml patients were stratified by age gender weight route of transmission duration of infection baseline hcv rna level and pegifn alphaa or rbv dosage hcv genotype was assessed in patients genotype b genotype a genotype ba rapid virological response rvr hcv rna negative at week complete early virological response cevr hcv rna negative at week eot response and svr were achieved in and patients respectively younger age lower weight and shorter speculated infection years were associated with higher svr rates vs x p value is less than vs x p value is less than vs x p value is less than svr significantly increased with treatment length and for is less than or equal to weeks weeks and is more than or equal to weeks respectively svr significantly increased with total cumulative treatment doses and for is less than or equal to and is more than or equal to of pegifn dose respectively and for is less than or equal to and is more than or equal to in rbv dose respectively in all patients less than of standard dose of rbv was not sufficient even if given enough pegifn is more than or equal to cumulative treatment dose in patients who achieved rvr chinese patients treated with peginterferon alphaa plus ribavirin have high rates of svr it is important to CR the target length of treatment and to continue the target dosage to achieve svr | MEDAL |
integrins itgs are key elements in cancer biology regulating tumor growth angiogenesis and lymphangiogenesis through interactions of the RT cells with the microenvironment moving from the hypothesis that itgs could have different effects in stage ii and iii CC we tested whether a comprehensive panel of germline singlenucleotide polymorphisms snps in itg genes could predict stagespecific time to tumor recurrence ttr a total of patients treated with fluorouracilbased chemotherapy at the university of southern california were included in this T0 wholeblood samples were analyzed for germline snps in itg genes using pcrrestriction fragment length polymorphism or direct dna sequencing in the multivariable analysis stage ii colon CA patients with at least one g S2 for itgb rs had higher risk of recurrence hazard ratio hr confidence interval ci p this association was also significant in the combined stage iiiii cohort hr ci p the predominant role of itgb rs in stage ii diseases was confirmed using recursive partitioning showing that itgb rs was the most important factor in stage ii diseases in contrast in stage iii diseases the combined analysis of itgb rs and itga rs allowed to identify three distinct prognostic subgroups p the interaction between stage and the combined itgb rs and itga rs on ttr was significant p this study identifies germline polymorphisms in itg genes as independent stagespecific prognostic markers for stage ii and iii colon cancer these data may help to select subgroups of patients who may benefit from itgtargeted treatments | MEDAL |
the oncogenic mechanisms and tumour biology underpinning clear cell sarcoma of the kidney ccsk the second commonest paediatric renal malignancy are poorly understood and currently therapy depends heavily on doxorubicin with cardiotoxic sideeffects previously we characterized the balanced tqp chromosomal translocation identified at that time as the only recurrent genetic aberration in ccsk this translocation results in an inframe fusion of the genes ywhae encoding ϵ and nutm with a somatic incidence of clinicopathological features of that cohort suggested that this aberration might be associated with higher stage and grade disease since no primary ccsk cell L1 exists we generated various stably transfected cell lines containing doxycyclineinducible hatagged ywhaenutm in order to study the effect of expressing this transcript ϵnutmexpressing cells exhibited significantly greater cell migration compared to isogenic controls gene and protein expression studies were indicative of dysregulated mapkpikakt signalling and by blocking these pathways using neutralizing antibodies the migratory advantage conferred by the transcript was abrogated importantly ccsk tumour samples similarly show upregulationactivation of these pathways these results support the oncogenic role of ϵnutm in ccsk and provide avenues for the exploration of novel therapeutic approaches copyright © pathological society of great britain and ireland published by john wiley sons ltd | MEDAL |
the hamster was used as a MM for investigating the effect of low moderate and high protein NI and casein on bone mineral content animals fed the low level of protein between and months of age had a reduction in the weight of all skeletal components measured with the exception of the diaphyseal portion of the long bones diaphyseal weight and calcium remained significantly lower when expressed as a percentage of body weight however urinary calcium excretion was not lower than that of animals consuming an adequate protein NI ingesting a high protein diet resulted in a significant increase in urinary calcium excretion and a reduced amount of both mineral and organic material in the diaphyses we conclude that LT consumption of a high protein ration led to the OD of a mild OP condition in the hamster which was limited to the diaphyseal portions of the long bones | MEDAL |
isolated anterior mitral leaflet prolapse unlike posterior prolapse is a difficult lesion to repair and may become a demanding surgical procedure we report our experience with a technique of a triangular resection of the anterior leaflet to repair isolated segmental anterior leaflet prolapse in patients this technique simplifies the repair procedure and is a safe and rapid procedure which allows excellent results | MEDAL |
primary UB adenosquamous carcinoma is extremely rare and only a few cases have been reported in english literatures its biological SMB remains unclear here we reported a yearold male patient with lower limb deep A-V thromboses associated with primary urinary bladder ASC a color ultrasonography showed RA stock total VT and right GSV thrombosis of lower limb CECT ct scan confirmed a à à cm enhancing mass within the urinary bladder along the RA lateral and posterior wall histopathological examination revealed ASC of urinary bladder with extensive infiltration of the muscle layer to the best of our knowledge this is the first report of primary UB adenosquamous carcinoma complicated with deep A-V thromboses in lower limb | MEDAL |
we report two unrelated women with gonadal dysgenesis and a pq and a pq balanced translocation respectively the patients were of normal stature and showed no phenotypic abnormality or malformation other than ovarian failure we are not aware of other reports of balanced autosomal translocations associated with gonadal dysgenesis in women the occurrence of chromosome anomaly and sterility in the two females may be coincidental however studies on mouse gametic progression indicate that balanced autosomal translocations can cause oocyte degeneration and reduction of reproductive lifespan on the basis of these observations we cannot exclude that the ovarian failure in our patients is the result of oocyte degeneration because of as yet unidentified consequences of the balanced translocations | MEDAL |
understanding how drugs are distributed in PL following local applications is important as local drug therapies are increasingly used to treat disorders of the inner ear the potential contribution of CSF fluid csf entry to perilymph homeostasis has been controversial for over half a century largely due to artifactual contamination of collected PL samples with csf measures of PL flow and of drug distribution following round window niche applications have both suggested a slow apically directed flow occurs along scala tympani st in the normal sealed cochlea in the present study we have used fluorescein isothiocyanatedextran as a marker to study PL kinetics in guinea pigs dextran is lost from perilymph more slowly than other substances so far quantified dextran solutions were injected from pipettes sealed into the lateral SC scc the cochlear apex or the basal turn of st T3 varying delays sequential PL samples were taken from the cochlear apex or lateral scc allowing dextran CSD along the perilymphatic spaces to be quantified R2 was low and findings were consistent with the injection procedure driving volume flow towards the cochlear aqueduct and with volume flow during perilymph sampling driven by csf entry at the aqueduct the decline of dextran with time in the period between i.t. and sampling was consistent with both a slow volume influx of csf nlmin entering the basal turn of st at the cochlear aqueduct and a csfperilymph exchange driven by pressuredriven fluid oscillation across the CA sample data also allowed contributions of other processes such as communications with adjacent compartments to be quantified the study demonstrates that drug kinetics in the basal turn of st is complex and is influenced by a considerable number of interacting processes | MEDAL |
percutaneous dilatational tracheostomy pdt in critically ill patients is often led by optical guidance with a bronchoscope this is not without its disadvantages therefore we aimed to study the feasibility of a recently introduced endotracheal tubemounted camera vivasightâ¢sl et view misgav israel in the guidance of pdt | MEDAL |
acid and ALP phosphatases were studied in different structures of the sheep uterus and placenta by semiquantitative histochemistry of frozen sections days of pregnancy and quantitative microfluorometry of freezedried sections days of pregnancy histochemically lowest activity for acid phosphatase was found in the uterine glands but biochemically it was lowest in the maternal caruncles histochemical acid phosphatase activity in the uterine glands and maternal caruncles increased on day of gestation however biochemically the activity decreased in the glands but did not NC in the maternal caruncles during pregnancy weak histochemical staining was found in the luminal epithelium on days and of gestation compared with other days whereas biochemically the activity was high at these times histochemically alkaline phosphatase activity in athe LE uterine PG and maternal caruncles was high and did not change during pregnancy fluorometrically the activity decreased in the luminal epithelium but it increased in the uterine glands and maternal caruncles histochemical data showed higher ALP phosphatase activity in the maternal caruncles and trophoblast than in the foetal cotyledons but biochemically no activity was detected in the trophoblast and foetal cotyledons | MEDAL |
we recorded electromyograms of slowtwitch red muscle SF and videotaped swimming in the largemouth bass micropterus salmoides during cruise burstandglide and cstart maneuvers by use of in vivo patterns of stimulation and estimates of CS in vitro power SO was measured at degrees c with the oscillatory work loop technique on slowtwitch fiber bundles from the midbody area near the soft dorsal fin power SO increased slightly with cycle frequency to a plateau of approximately wkg at hz encompassing the normal range of tailbeat frequencies for steady swimming approximately hz SP output declined at cycle frequencies simulating unsteady swimming burstandglide hz cstart hz however activating the muscle at hz did significantly increase the net work done compared with the work produced by the inactive muscle work done by the viscous and elastic components thus this study provides further insight into the apparently paradoxical observation that RM can contribute little or no SP and yet continues to show some recruitment during unsteady swimming comparison with published values of SP requirements from oxygen consumption measurements indicates a limit to steady swimming speed imposed by the maximum SP available from RM | MEDAL |
the article deals with the findings obtained in examining a total of people presenting with varicose disease of the lower extremities the examination included a clinical PCS examination and coloured duplex scanning of the lowerlimb veins the examination was carried out in OP conditions whereas surgical treatment was performed either outpatiently or at a oneday hospital revealing complete valvular insufficiency of the great saphenous vein was followed by surgical treatment intraoperative stem catheter scleroobliteration sso or shortterm screening and postoperative puncture sclerotherapy detecting partial valvular insufficiency of the greater saphenous vein pathological saphenofemoral reflux only in the vertical position was managed by applying the principle of the socalled incremental radicalism i e the patients were subjected to the procedure of puncture sclerotherapy of varicose veins on the crus in ambulatory conditions to be followed by secondary ultrasonographic scanning with permanent dynamic followup the cases of preserved reflux along the greater saphenous vein were subjected to sclerosurgical management valvular insufficiency of perforating veins was coped with by minimally invasive surgical interventions subtotal endoscopic dissection of incompetent perforating veins suprafascial ligation from a miniaccess and echosclerotherapy of the perforating veins also performed were histological and histochemical studies of the portions the greater RSV and surrounding tissues procured during crossectomy of the saphenofemoral anastomosis in patients who had endured preliminary puncture sclerotherapy on the crus good PET and cosmetically pleasing outcomes were achieved in the majority of cases in of the patients with partial valvular insufficiency of the GSV we managed to avoid operations since repeat ultrasonographic study revealed no pathological venovenous shunts | MEDAL |
topiramate a structurally novel anticonvulsant and phenytoin were evaluated in a rat model of ischemiainduced epilepsy in this model a transient GCI is induced by cardiac compression by precisely controlling the exp conditions the procedure causes reproducible neurological deficits that include audiogenic ES the seizures can be broadly separated into three types reflecting the degree of severity wild running clonic seizures and tonic extension seizures of the forelimbs and hindlimbs topiramate and phenytoin blocked all three types of seizures calculated ed values for topiramate hr T3 oral administration were and mgkg for blockade of TE seizures clonic seizures and wild running respectively corresponding ed values for phenytoin were and mgkg these results support the concept that the anticonvulsant activity of these drugs is due primarily to an ability to block the spread of seizures | MEDAL |
the restriction of herpes simplex virus type hsv mRNA in nonpermissive mu resident peritoneal macrophages respmo was analyzed at the level of transcription using nuclear runon assays we established that transcription of a representative gene from each hsv kinetic class was initiated in both respmo and permissive vero cells using transient expression assays we observed that the promoters of two early genes were G1 by the immediateearly protein icp in respmo however the level of TA of these promoters by another immediateearly protein icp was markedly reduced in respmo furthermore the synergistin enhanced dumbbellshaped mass with its smaller part occupying the left cerebellopontine angle and its larger part in left parapharyngeal space the mass was totally removed with a left mastoidectomy and suboccipital craniotomy the DM was closed with anterior lamina of the rectus sheath and the large defect of the neck was obliterated with free rectus abdominis muscular flap the postoperative course was uneventful except for some sputum aspiration and hoarseness he underwent surgical mediation and cricopharyngeal myotomy for his left paralyzed vocal cord and both speech and swallowing CF improved quite well this paper reports our experience in managing this dumbbellshaped neurinoma of the jugular foramen | MEDAL |
the worldwide emergence of antibioticresistant bacteria poses a serious threat to human health to understand the mechanisms of the resistance is extremely important to the control of these bacteria in the current study proteomic methodologies were utilized to characterize om proteome of escherichia coli with NAL na resistance the om proteins tolc ompt ompc and ompw were found to be upregulated and fadl was downregulated in the naresistant e coli strains the changes at the level of protein expression were validated using western blotting furthermore the possible roles these altered proteins played in regulation of na resistance were investigated using GM strains with the deletion of these genes the results obtained from PET characterization of these genetically modified strains suggest that tolc and ompc may play more important roles in the control of na resistance than other om proteins identified to gain better understanding of the mechanisms of na resistance we also characterized the role of the twocomponent system envzompr which is responsible for the regulation of ompc and ompf expression in response to na resistance using their genetically modified strains our results suggest that ompf and the envzompr are also important participants of the pathways regulating the na resistance of e coli | MEDAL |
the paper presents a d filter chip employing both mechanical and dielectrophoretic dep filtration and its corresponding microfabrication techniques the device structure is similar to a CP capacitor two planar electrodes made from a SS mesh and bonded on both sides of a glass frame filled with round silica beads the solution with the suspension of particles flows through both the meshelectrodes and silica beads filter the top SS mesh with openings of mum and wires of mumthickness provides the first stage of filtration based on mechanical trapping a second level of filtration is based on dep by using the nonuniformities of the electric field generated in the capacitor due to the nonuniformities of the dielectric medium the filter can work also with dc and ac EFs the device was tested with yeast cells saccharomyces cerevisae and achieved a maximal trapping efficiency of at an applied ac Vc of v and a flow rate of mlmin from an initial concentration of cells of x cellsml when the applied frequency was varieted in the range between and khz a minimal value of capture efficiency was notticed at khz when yeast cells exhibit negative dep and the cells are repelled in the space between the beads | MEDAL |
the extent and time course of changes in selected procoagulant and anticoagulant AF were investigated in patients undergoing elective abdominal aortic surgery the coagulation AF were measured preoperatively and on days two four and six postoperatively it was found that there were no significant changes outside the normal range in prothrombin time partial thromboplastin time or thrombin clotting time however there were C1 increases in the procoagulants fibrinogen factor viii coagulant factor viiiragvon willebrand factor and in alpha antitrypsin over the same time there were marked decreases in the naturally occurring anticoagulants protein c and AT iii and in AMG these changes implied that the patients were hypercoagulable in the postoperative period the maximum changes in the procoagulants occurred on either postoperative day two or day four the maximum changes in the natural anticoagulants occurred on postoperative day two there were no significant changes in factor v FX alpha antiplasmin or platelet aggregability the timing of the changes coincided with a period of high risk of perioperative myocardial infarction in this group of patients thus it is possible that postoperative hypercoagulability contributes to the OD of coronary i.a. thrombosis and myocardial infarction following abdominal aortic surgery | MEDAL |
eosinophilic esophagitis eoe is characterized by the inflammation of the esophagus and the infiltration of eosinophils into the esophagus leading to PS such as dysphagia and stricture formation systemic immune indicators like eotaxin and fibroblast growth factor were evaluated for possible synergistic pathological effects moreover blood cells local tissue and plasma from eoe and control subjects were studied to determine if the localized disease was associated with a SVR effect that correlated with presence of eoe disease | MEDAL |
endoplasmic reticulum erassociated degradation erad is an essential part of an erlocalized protein qualitycontrol system for eliminating terminally misfolded proteins recent studies have demonstrated that the erad machinery is conserved among yeast animals and plants however it remains unknown if the plant erad system involves plantspecific components here we report that the arabidopsis ethyl methanesulfonatemutagenized brassinosteroidinsensitive suppressor ebs gene encodes an er membranelocalized erad component that is highly conserved in land plants lossoffunction ebs mutations prevent erad of BR insensitive bri and bri two erretained mutant variants of the cellsurface receptor for brassinosteroids brs as a result the two mutant receptors accumulate in the er and consequently leak to the plasma membrane resulting in the restoration of br sensitivity and phenotypic suppression of the bri and bri mutants ebs accumulates under er AS and its mutations lead to hypersensitivity to er and salt stresses ebs interacts with the er membraneanchored ubiquitin ligase arabidopsis thaliana hmgcoa reductase Kd a athrda one of the central components of the arabidopsis erad machinery and an ebs mutation destabilizes athrda to reduce polyubiquitination of bri taken together our results uncover a plantspecific component of a plant erad pathway and also suggest its likely biochemical CF | MEDAL |
plants containing CT cts may hold promise as alternatives to synthetic anthelmintic ah drugs for controlling gastrointestinal nematodes gins however the structural features that contribute to the ah activities of cts remain elusive this T0 probed the relationships between ct structures and their ah activities eighteen plant resources were selected on the basis of their diverse ct structures from each plant resource two ct fractions were isolated and their in vitro ah MICs were measured with the larval exsheathment inhibition assay which was applied to haemonchus contortus and trichostrongylus colubriformis calculation of mean ec values indicated that h contortus was more susceptible than t colubriformis to the different fractions and that the f fractions were less efficient than the f ones as indicated by the respective mean values for h contortus f ± μgml and f ± μgml and for t colubriformis f ± μgml and f ± μgml the results showed that the ah activity against h contortus was associated with the monomeric subunits that give rise to prodelphinidins p and with ct polymer size p however for t colubriformis ah activity was correlated only with prodelphinidins p these results suggest that cts have different modes of action against different parasite species | MEDAL |
the formation of carbonic anhydrase b associates ph urea concentration m k was studied as a function of protein concentration and time by nuclear magnetic resonance spectroscopy spin diffusion method it was found that the association process proceeds in two steps the first step is relatively fast and cannot be controlled by our methods during this step persistent units are built these consist of protein molecules that are able to interact with solvent molecules and with each other when protein solution contains m of urea persistent units are relatively small two three protein molecules and their mobility matches one of a single protein the second step is slower and throughout this step large structures are formed from persistent units the parameters g and s which characterize spin diffusion in a protein and a solvent respectively when spin DWI excitation happens away from nmr spectral signals are related to the probable size distribution of proteinsolvent associates and are determined by their collective properties | MEDAL |
a survey of CA indicated that the organism was present at all CS sampled in a rain forest stream and in nearshore coastal waters of puerto rico in the rain forest watershed no relationship existed between densities of fecal coliforms and densities of c albicans at two pristine CS in the rain forest watershed both c albicans and escherichia coli survived in diffusion chambers for D2 periods of time in nearshore coastal waters c albicans and e coli survival times in DWI chambers were enhanced by effluent from a rum distillery the rum distillery effluent had a greater effect on e coli than on c albicans survival in the DWI chambers these studies show that neither e coli nor c albicans organisms are good indicators of recent fecal contamination in tropical waters it further demonstrates that pristine freshwater environments and marine waters receiving organic loading in the tropics can support densities of c albicans which may be a health hazard | MEDAL |
nucleotide CS comparison of the internal region of the ati gene of members of the OPV genera revealed that this gene is variable among different species although within members of the same species it is considered to be well conserved previous studies indicated that there is genetic R2 in the ati gene among some brazilian vaccinia virus strains to further investigate this variability we performed molecular analysis of the internal region of the ati gene of eight brazilian vaccinia virus strains while the internal region of this gene in one strain was similar to the western reserve strain four strains presented two blocks of deletions in the analyzed region and the ati gene was almost entirely deleted from three other strains these findings demonstrate that there is genetic polymorphism within the ati gene among different brazilian vaccinia virus strains | MEDAL |
Subsets and Splits
No community queries yet
The top public SQL queries from the community will appear here once available.