text
stringlengths 10
10.1k
| source
stringclasses 1
value |
|---|---|
the objective of this prospective comparative study was to investigate the relationship of US measurements of the endometrium at the time of peak follicular maturation to conception outcome in patients treated for LP defects as determined by outofphase endometrial biopsies treatments for LP defects included progesterone supplementation with or without folliclematuring drugs depending on whether the patient attained a F2 of at least mm and a serum estradiol of pgml no differences in pregnancy rates were found by the thickness of endometrium vs or mm or echo DP in any of the treatment modalities thus contrary to findings in stimulated cycles for in vitro fertilization endometrial thickness at the time of peak follicular maturation is not predictive of outcome in patients treated for LP defects in NK cycles | MEDAL |
the socalled percutaneous dilatational tracheostomyessentially a minimally invasive puncture methodinserting the tracheal cannula by a modified seldingertechnique is an alternative method to the conventional operative tracheostomy the PDT was evaluated in a prospective trial june january on consecutive surgical n medical n and neurologicalneurosurgical n critically ill patients m f age years with need for prolonged mechanical ventilation after an average duration of endotracheal intubation of days the procedure was endoscopically guided and controlled via the ET an mm cannula was inserted in each case eight patients had severe thrombocytopenia or pltmicrol the percutaneous tracheostomy was always performed with success the average procedure duration was minutes the perioperative complications were one patient died of acute cardiac failure independent from the method of tracheostomy one sustained a temporary subcutaneous emphysema and one a minor bleeding during a mean duration of cannulation of days only one bleeding from the skin SM was observed postoperatively infection of stoma site misplacement of cannula rupture of the tube cuff and PTX were not noticed on decannulated patients stenosis of the tr was not found in a period of weeks following the tracheostomy as a bedside procedure the PDT is safe and quick and should therefore be the method of choice for critically ill patients who require a tracheostomy | MEDAL |
phenotypic properties and speciesspecific pcr tests based on the nuc gene of staphylococcus intermedius and s aureus and a conserved region of s rdna were used to identify s intermedius and four s aureus isolated from samples of dogs during routine diagnostics four s pseudintermedius strains used for control purposes reacted positively with the s intermedius nuc pcr showing the close relationship between both species investigating the s intermedius and four s pseudintermedius strains for the prevalence of the exfoliative toxin siet encoding gene yielded the presence of the gene for of the s intermedius and two of the s pseudintermedius strains partial sequencing of the toxin gene of a single s intermedius strain and comparing this sequence with that obtained from genbank revealed an almost CR identity the presence of the exfoliative toxin gene could mainly be found among s intermedius isolated from skin and wound infections and from otitis externa possibly indicating a role of this toxin for the clinical PS | MEDAL |
sterolregulated ubiquitination is an obligatory step in erassociated degradation erad of hmg coa reductase a ratelimiting enzyme in cholesterol synthesis accelerated degradation of reductase one of several strategies animal cells use to limit production of cholesterol requires sterolinduced IB of the enzyme to er membrane proteins called insigs once formed the reductaseinsig complex is recognized by a putative membraneassociated ubiquitin ligase e that mediates the reductase ubiquitination reaction here we show that gp a membrane bound e binds to insig and is required for sterolregulated ubiquitination of reductase in addition gp couples regulated ubiquitination to degradation of reductase by IB to vcp an atpase that plays a key role in recognition and degradation of erad substrates the current results identify gp as the e that initiates sterolaccelerated Kd of reductase and insig as a bridge between gpvcp and the reductase substrate | MEDAL |
complex chromosome aberrations any exchange involving three or more breaks in two or more chromosomes are effectively induced in IP blood lymphocytes pbl after SE to low doses mostly single particles of densely ionising highlinear energy transfer let alphaparticle radiation the complexity when observed by multiplex fluorescence in situ hybridisation mfish shows that commonly four but up to eight different chromosomes can be involved in each rearrangement given the territorial organisation of chromosomes in interphase and that only a very small fraction of the nucleus is irradiated by each alphaparticle traversal the aim of this T0 is to address how aberrations of such complexity can be formed to do this we applied theoretical cycle analyses using mfish paint detail of pbl in their first cell division after exposure to highlet alphaparticles in brief cycle analysis deconstructs the aberration observed by mfish to make predictions as to how it could have been formed in interphase we propose from this that individual highlet alphaparticleinduced complex aberrations may be formed by the misrepair of damaged chromatin in single physical CS within the nucleus where each site is consistent with an area corresponding to the interface of two to three different chromosome territories limited migration of damaged chromatin is allowed within this area complex aberrations of increased size reflecting the path of alphaparticle nuclear intersection are formed through the sequential linking of these individual sites by the involvement of common chromosomes | MEDAL |
myotonia congenita and periodic paralyses are hereditary skeletal muscle channelopathies in these disorders various channel defects in the sarcolemma lead to a severely disturbed membrane excitability of the affected skeletal muscles the clinical picture can range from severe myotonic reactions eg masseter spasm opisthotonus to attacks of weakness and paralysis provided here is a short overview of the pathomechanisms behind such wideranging phenotypic presentations in these patients followed by recommendations concerning the management of anesthesia in such populations | MEDAL |
a year old girl with ebsteins anomaly and wolffparkinsonwhite syndrome without SVT ART underwent closure of an atrial septal defect and TV commissurotomy her postoperative course was complicated by her first episodes of recurrent debilitating paroxysmal SVT ART severe TI with low cardiac output necessitated a repeat intracardiac T0 at reoperation the patient underwent successful TVR with concomitent cardiac mapping and division of the bundle of kent in patients with HR disease requiring intracardiac repair who also have WPW elective surgical division of the anomalous bundle is recommended whether or not preoperative attacks of ART have occurred | MEDAL |
infiltration of spinal nerve roots and IP SN by macrophages and t cells are rather consistent immunopathologic findings in patients with guillainbarré syndrome gbs and chronic inflammatory demyelinating polyradiculoneuropathy cidp chemokines play a central role in recruitment of leukocytes to inflamed tissue chemokines have been implicated in the pathogenesis of the EAN ean which represents an animal MM of gbs but the role of chemokines in gbs and cidp is not clear since chemokines may be released into csf from inflamed spinal nerve roots we studied the concentrations of the chemokines mcp mipbeta mipbeta ip sdfalpha rantes and slc in the csf by sandwich elisa in patients over the course of gbs and cidp before and after immunomodulatory treatment controls consisted of patients with noninflammatory neurological disorders patients examined in the acute phase of gbs prior to treatment with intravenous high dose immunoglobulins ivig had elevated csf C2 of mcp a chemoattractant for blood monocytes and dendritic cells and ip a chemoattractant for t cells patients with cidp examined prior to immunomodulatory treatment had elevated csf levels of mipbeta a chemoattractant for mature dendritic cells naïve and recently G1 t cells and ip levels of mipbeta tended to decreased during followup in those cidp patients responding favorably to immunomodulatory treatment csf C2 of mcp and ip correlated with the csfplasma albumin ratio in both gbs and cidp patients in cidp patients csf C2 of mipbeta also correlated with the csfplasma Al ratio these data implicate mcp and ip in the pathogenesis of gbs and ip and mipbeta in the pathogenesis of cidp | MEDAL |
cationic lipiddna complexes are used as gene transfer vehicles in molecular biology and potentially in human GT in recent synchrotron xray scattering studies the molecular structure of such selfassembling aggregates was elucidated a rich polymorphism of lamellar hexagonal lamellarcolumnar and micellar mesophases was found in this article we describe composite phases of cationic lipid mixed with hyaluronic acid and DS which likewise form IC lamellar complexes heterogeneous phases of lipiddextran sulfate mixed with lipiddna exhibit macroscopic phase separation when dextran sulfate is added to preformed cationic lipid dna complexes the latter are dissolved in favor of the lipidpolyelectrolyte phases we investigated the kinetics of the dna replacement by dextran sulfacopic breast augmentation is believed to be a safe alternative technique with excellent results | MEDAL |
this T0 was designed to investigate the effect of VE growth factor adenovirus advegftransfected mesenchymal stem cells msc on improving EF in diabetic rats fortyfive SD rats were injected with streptozotocin to develop type diabetes whereas served as NCs diabetic rats were randomly divided into CG rats that underwent intracavernous injection with phosphatebuffered saline dmpbs unmodified mscs dmmsc and advegftransfected mscs dmvmsc normal controls received intracavernous i.t. of pbs four weeks T3 injection erectile function was measured by CN ES penile tissue was harvested for histology and enzymelinked immunoassay prior to i.t. high expression of vegf was confirmed in advegftransfected mscs by enzymelinked immunoassay four weeks T3 injection the EF as well as the content of smooth muscle and endothelium in CC increased significantly in the mscinjected CG compared with the dmpbs group there was a significant improvement of erectile function the content of smooth muscle and endothelium and the vegf concentration in the corpus cavernosum in the dmvmsc group compared with the dmmsc group our T0 validates the effect of intracavernous injection of mscs for diabetesassociated erectile dysfunction in an animal model the combined strategy of msc injection with vegf gene therapyenhanced therapy of mscs for the treatment of diabetesassociated erectile dysfunction | MEDAL |
magnetic resonance imaging demonstrated coexistent CNS system abnormalities in of patients with ONH based on their associated neuroradiological findings these patients were placed into one of five categories group isolated ONH group absence of the septum pellucidum group PP ectopia group hemispheric migration anomalies and group intrauterineperinatal hemispheric injury PP ectopia group and cerebral hemispheric abnormalities CG and were found to be highly predictive of PIT hormone deficiency and neurodevelopmental deficits respectively isolated absence of the SP pellucidum group was associated with normal neurodevelopmental and endocrinologic function thinning or agenesis of the corpus callosum was predictive of neurodevelopmental problems only by virtue of its frequent association with CBF hemispheric abnormalities magnetic resonance imaging can now be used to provide specific prognostic information regarding the likelihood of neurodevelopmental deficits and PIT hormone deficiency in infants with optic nerve hypoplasia the prevailing notion of septooptic dysplasia as a distinct nosologic entity should be reconsidered | MEDAL |
the T0 aimed to identify the lateral heterogeneity of soil physicochemical properties in riparian zones and its underlying drivers during NK restoration after agricultural abandonment abandoned farmlands after year natural restoration within m from the edges on both sides of liaohe river were selected as the study area soil physicochemical properties of four lateral buffers m m m and m from river edge respectively along riparian zones were measured the results showed that riparian soils were characterized by high sand content and poor soil nutrients soil silt content OC oc cation exchange capacity cec total nitrogen tn and available nitrogen an increased laterally with increasing distance from river edge while soil sand content decreased total phosphorus tp and AP ap are not spatially autocorrelated soil oc tn an and cec along UP and midstream reaches showed negative spatial autocorrelation along the lateral gradients and positive along downstream reach ALT distance from river edge and distance from nearest farmland were the pronounced AF affecting soil physicochemical properties in this T0 | MEDAL |
we report a scalably synthesized wobivo coreshell nanowire photoanode in which bivo is the primary lightabsorber and wo acts as an electron conductor these coreshell nanowires achieve the highest product of CS absorption and charge separation efficiencies among bivobased photoanodes to date and even without an added catalyst produce a photocurrent of macm under simulated sunlight and an incident photontocurrent conversion efficiency of â¼ at nm both at a potential of v versus rhe | MEDAL |
the studies proved that males and females who work outdoors in far north demonstrate CS of thermoregulation and such associated systems as primarily cardiovascular and respiratory ones lower tolerance of the workers to physical CS shows more intensive impact of work in such regions on physiologic functioning the studies determined peculiarities of pulmonary circulation in the inhabitants of far north those peculiarities are caused by cooling and low absolute humidity functional strain in the examinees appeared to depend on weather conditions of far north | MEDAL |
the current T0 investigated AF related to specific phobia domains and differences in patterns among african american and caucasian american adults subjects were african americans and caucasian americans who completed the fear survey schedulesecond edition fssii fears related to TPS phobia domains were first examined with frequencies differing between african american and CA samples on three of the six TPS phobia domains a confirmatory factor analysis was conducted to determine the patterns of specific phobias among the african american sample the trimmed MM for the african american sample included natural environment animal and social anxiety TPS phobia factors as latent exogenous variables data from the CA sample provided a poor fit to this model instead the trimmed model for the CA sahospital in several ways such as increasing revenues filling unused capacity and solidifying relationships with the physician and patient communities building rapport with physicians and the community ultimately brings economic value to the hospital to service this new market it is necessary to modify current systems and processes and develop new services the Az most likely to be developed are logistics service centers client services physician connectivity billing marketing sales and finance reporting developing efficient customerrelated services is a key to reaping the benefits | MEDAL |
interfacial contact of twodimensional graphene with threedimensional metal electrodes is crucial to engineering highperformance graphenebased nanodevices with superior performance here we report on the development of a rapid nanowelding method for enhancing properties of interface to graphene buried under metal electrodes using a focused EB induced deposition febid high SE electron irradiation activates 2DE graphene structure by generation of structural defects at the interface to metal contacts with subsequent strong bonding via febid of an atomically thin graphitic interlayer formed by low SE AA electronassisted dissociation of entrapped hydrocarbon contaminants comprehensive investigation is conducted to demonstrate formation of the febid graphitic interlayer and its impact on contact properties of graphene devices achieved via strong electromechanical coupling at graphenemetal interfaces reduction of the device electrical resistance by â¼ at a dirac point and by â¼ at the gate Vc far from the dirac point is obtained with concurrent improvement in thermomechanical reliability of the contact interface importantly the process is rapid and has an excellent insertion potential into a conventional fabrication workflow of graphenebased nanodevices through singlestep postprocessing modification of interfacial properties at the buried heterogeneous contact | MEDAL |
in order to compare the neuromuscular effects following rocuronium mg kg and atracurium mg kg children aged from to months were studied under the same anaesthetic conditions T3 induction of anaesthesia with etomidate and FE NM blockade was monitored by recording the electromyographic response of the adductor pollicis muscle to a supramaximal trainoffour tof stimulation of the ulnar nerve at hz for s at s intervals intubation was performed when more than muscle EC50 was achieved thereafter anaesthesia was maintained with nitrous oxide in oxygen and isoflurane endtidal mean ON of action was significantly faster following rocuronium s mean sd compared with atracurium s clinical duration with ROC was min and thus significantly shorter than that of atracurium which was min a statistically significant difference between rocuronium and atracurium also had been found for REC of t to and as well as for the time taken to a tof ratio of the recovery index for ROC and atracurium was not significantly different with min and min respectively thus rocuronium may be more advantageous than atracurium for shortlasting surgical procedures in young children | MEDAL |
human apobecb ab like other apobec members is a cytosine deaminase which causes hypermutation of single stranded genome recent studies have shown that ab is predominantly elevated in multiple cancer tissues and cell lines such as the bladder cervix lung head and neck and breast upregulation and activation of ab in developing tumors can cause an unexpected cluster of mutations which promote cancer OD and progression the cellular proteins which facilitate ab function through direct or indirect interactions remain largely unknown in this study we performed lcmsbased proteomics to identify cellular proteins which coimmunoprecipitated with ab our results indicated a specific interaction of ab with hnrnp a heterogeneous nuclear ribonucleoprotein this interaction was verified by coimmunoprecipitation and was found to be rnadependent furthermore ab and hnrnp a colocalized as evident from IFA | MEDAL |
the endostatin precursor collagen xviii is expressed at high C2 in human livers the main source being HCs we have studied the regulatory elements in the promoter of the cola gene that directs the transcription of the nc variant of collagen alphaxviii which is the main form expressed in the liver the flanking region of cola gene was cloned and a series of deletions from bp to bp were linked to the luciferase reporter gene transfection experiments in hepg cells allowed to identify a silencerlike PE containing putative hnf and hnf sites and activator elements containing stretches of gcrich sequences another putative hnf site in close apposition to a nfctf site was localized UP of the silencerlike PE cotransfection experiments showed that the cola promoter was transactivated by sp and hnfalpha gelshift analyses showed that hnf nfctf and splike CS specifically recognized nuclear AF supershift experiments indicated that hnfbeta was the major form of hnf interacting with the hnfnf site the welldifferentiated hepatoma cell line mhatfs transfected with a truncated form of hnfbeta which competitively blocks hnf transactivating activity expressed the colagene at a very low level taken together these data strongly suggest that cola is a liverspecific gene furthermore gelshift analyses performed with nuclear factors prepared from welldifferentiated HCC carcinomas showed increased hnfnf CBA compared with normal livers consequently the precursor of endostatin might be differently expressed according to the differentiated andor transformed state of HCs | MEDAL |
in the present work double water compatible molecularly imprinted polymers dwcmips with water compatible core and hydrophilic polymer brushes were prepared by reversible additionfragmentation chain transfer precipitate polymerization raftpp and applied as SPE spe sorbent for selective preconcentration and TPS recognition of triazines in water samples the dwcmips employed as spe sorbent presented much higher extraction efficiency for four triazines in AQ media based on the double water compatible property the validated method was also successfully applied to tap water and river water sample analysis and satisfactory recoveries were attained such as with the precision of for four triazines at μgl the dwcmipsspe proves to be a highly ERP cleanup and enrichment method for simultaneous separation and sensitive determination of triazines in complicated water samples | MEDAL |
a simple and rapid spectrophotometric method with flow injection is proposed for determination of diphenhydramine hydrochloride in some single tertiary alkylamine pharmaceutical preparations capsule and syrup it is based on ion pair formation with bromocresol green in a ph buffer which a yellow ion pair compound is extracted into chloroform layer an AQ layer containing excess BCG is injected into a carrier stream of m borax solution and absorbance of the stream is continuously monitored at nm diminution of the BCG is related to an amount of diphenhydramine hydrochloride that can be evaluated from a calibration graph established by a plot of diphenhydramine hydrochloride concentration micro gm l and peak height mv optimization will be discussed the calibration graph is linear over the concentration ranges of and micro gm l a throughput of i.v. h can be obtained application to assay for capsule and syrup samples has been demonstrated the method is validated by comparing the results with hplc analyses | MEDAL |
we present a new method for analyzing the deltac isotopic composition of several oxygenated volatile organic compounds ovocs from direct sources and ambient atmospheric samples guided by the requirements for analysis of trace components in air a gas chromatograph IRMS gcirms system was developed with the goal of increasing sensitivity reducing deadvolume and peak band broadening optimizing combustion and water removal and decreasing the split ratio to the IRMS irms the technique relies on a twostage preconcentration system a lowvolume capillary reactor and water trap and a balanced REF gas delivery system the instruments measurement precision is to per thousand sigma and results indicate that negligible sample fractionation occurs during gas sampling measured deltac values have a minor dependence on sample size linearity for AC was per thousand ng c and was best over ng c sensitivity is approximately times greater than similar instrumentation designs incorporates the use of a diluted working REF gas co and requires collection of ng c to produce accurate and precise results with this PCD limit a l sample of ambient air provides sufficient carbon for isotopic analysis emissions from vegetation and VH exhaust are compared and show clear differences in isotopic signatures ambient samples collected in metropolitan miami and the everglades national park can be differentiated and reflect multiple sources and sinks affecting a single sampling location vehicle exhaust emissions of ethanol and those collected in metropolitan miami have anomalously enriched deltac values ranging from to per thousand we attribute this result to ethanols origin from corn and use as an additive in automotive fuels | MEDAL |
trauma is a common cause of death in neonatal new zealand sea lion pups and subadult male sea lions have been observed picking up and violently shaking some pups in humans axonal injury is a common result of traumatic brain injury and can be due to direct trauma to axons or to ischaemic damage AA to trauma shaken baby syndrome which has been described in human infants is characterised by retinal and intracranial subdural haemorrhages and has been associated with ai to the BB spinal cord and optic nerve this study identifies mechanisms of traumatic brain injury in new zealand sea lion pups including impact injuries and shakingtype injuries and identifies gross lesions of head trauma in sea lion pups found dead at a breeding site in the auckland islands despite the high frequency of such gross lesions only three of the pups had died of traumatic brain injury observational studies confirmed that shaking of pups occurred but none were shown to die as a direct result of these shaking events axonal injury was evaluated in all pup brains using βamyloid precursor protein immunohistochemistry immunoreactive axons were present in the brains of all pups examined including seven with V1 ai and two with diffuse axonal injury but the severity and pattern of injury was not reliably associated with death due to traumatic brain injury no dead pups had the typical combination of gross lesions and immunohistochemical findings that would conform to descriptions of shaken baby syndrome axonal injury was present in the optic SN of most pups irrespective of cause of death but was associated with ischaemia rather than trauma | MEDAL |
interferon consensus sequence binding protein icsbp was first identified as a transcription factor of the interferon ifn regulatory factor family irf which regulates expression of ifndependent genes by binding to dna at TPS sites ifnstimulated responsive elements analysis of icsbpdeficient mice showed hematologic alterations similar to chronic myelogenous leukemia cml in humans and suggested a novel role for icsbp in regulating proliferation and differentiation of hematopoietic progenitor cells here we show that icsbpmrna expression is impaired in human myeloid leukemias of cml patients and of patients with AML leukemia aml showed very low or absent transcript numbers of icsbp in contrast only of NV showed low transcription of icsbp p both for cml and aml values the lack of expression was not associated with lack of lymphatic cells which normally have been shown to express icsbp at the highest level more detailed analysis showed an absence of icsbpmrna also in sorted b cells derived from cml patients to analyze whether icsbp may be induced in leukemic cells ex vivo experiments using a known inducer of icsbp Th1 were performed ex vivo treatment of primary cml cells using Th1 resulted in induction of icsbp transcripts furthermore samples of cml patients during ifnalpha treatment were analyzed in of cml patients icsbpmrna was inducible upon in vivo treatment with ifnalpha but decreased with progression of cml stable transfection of k cell line with icsbp led to no difference in bcrabl expression in vitro although two patients showed an inverse correlation between bcrabl and icsbp in vivo these data suggest that lack of icsbp may have an important role also in human myeloid leukemogenesis | MEDAL |
the administration of the aminonucleoside of puromycin pan to rats causes the nephrotic syndrome that is associated with an acute decline in renal CF and an interstitial infiltrate we examined whether essential fatty acid deficiency efad which inhibits macrophage infiltration in glomerulonephritis affects paninduced RD both control and efad rats developed PU that resolved over d after pan administration there was a prominent infiltration of macrophages in rats fed a normal diet the infiltrate was prevented by the efad diet the absence of a macrophage interstitial infiltrate was associated with a significantly higher cin in the efad rats than in controls at d versus p less than mlminkg bw in addition cpah fell to less than mlminkg bw by day in controls but remained the same as normal in the efad after administration of pan to control rats there was no increase in urinary thromboxane excretion or an increase in glomerular thromboxane production furthermore the effect of efad could not be mimicked by the administration of a thromboxane synthase inhibitor irradiationinduced leukopenia in rats on a ND markedly improved glomerular filtration and RBF in acutely nephrotic rats efad prevents the interstitial cellular infiltrate and the renal ischemia associated with experimental nephrosis the recruitment of mononuclear cells into the kidney following pan directly contributes to the decline in renal CF | MEDAL |
channa striata bloch is a fresh water fish belonging to the family channidae the stripped snakehead fish possesses wide range of medicinal properties in view of traditional use of c striata for wound healing the present study was undertaken to investigate the beneficial effects of orally administered freeze dried AQ extract of channa striata aecs in experimentally induced gastric ulcers in wistar rats aspirin induced ulcerogenesis in pyloric ligation MM was used for the assessment of antiulcer activity and ranitidine mgkg was employed as the standard drug the various gastric parameters like volume of gastric juice ph free and total acidities ulcer index and C2 of antioxidant enzymes like catalase superoxide dismutase and lipid peroxidation marker malondialdehyde were determined aecs at concentrations of and wv significantly decreased the volume of gastric juice and increased the levels of catalase while considerable decrease in free and total acidities and increase in superoxide dismutase were observed with the treatment of standard drug and aecs wv all the test doses of aecs markedly decreased ulcer index and malondialdehyde compared to the standard drug whereas aecs wv did not alter volume of gastric juice ph free and total acidities catalase and superoxide dismutase from these findings it can be concluded that aecs is devoid of acid NT effects at lower doses and possesses antisecretory and antiulcer MICs and this could be related to its antioxidant mechanism | MEDAL |
this paper focused on methodological issues in the epidemiology of multiple primary cancer especially on methods to evaluate the frequency of a second cancer the incidence density is the most widely used measure in CA epidemiology however the measure of incidence might be biased to some extent because it might be affected by extraneous factors which are related to the PCD of the second CA a better alternative is the measure of the prevalence of occult CA the authors have formulated the functional relationship between the prevalence of occult CA and some variables which can be obtained from ordinary epidemiologic observations such as the ID the growth function and the CSD of the diameters of the cancers which were clinically diagnosed the formula was then applied to an epidemiologic T0 of multiple primary cancer in the digestive system all the patients with cancer of the digestive system except the stomach treated in the national cancer center hospital between and constituted the study group among male and female patients gastric cancers were found as the second primary CA in nine males and one female within months T3 the first cancers were detected all the first cancers in these cases were esophageal cancers the expected numbers of second gastric cancers were calculated as in males and in females by using the formula the observed number exceeded significantly the expected number in males p value which suggested that esophageal and gastric cancers might share some risk factor | MEDAL |
lipopolysaccharide lps induced tumour necrosis factor alpha tnf | MEDAL |
the authors examined the frequency of the last remaining tooths positions in both arches and they found the canine in the lower arch in and the M2 in of the second molar in single remaining tooth situations the rate of wearing denture is the cariological status of these teeth is negligible in relation to the bad periodontal health conditions which are not influenced essentially by crowns or wearing dentures | MEDAL |
to assess and compare the sensitivity to change of the EU league against rheumatism sjögrens syndrome disease activity index essdai with that of other primary sjögrens syndrome ss SLEDAI indexes | MEDAL |
in vitro tensile bond strengths of composite to porcelain treated by acid etching or sandblasting were measured a hf gel was applied for or seconds at degrees c alo particles were applied as wet dry dry or dry microns average roughness ra was measured by profilometry before and T3 each treatment both longer etching time and sandblasting using alo with larger particle sizes produced increased surface roughness of porcelain etching with hydrofluoric acid gel produced higher bond strengths than sandblasting with a series of alo particles etching longer than seconds produced increased cohesive failures in porcelain whereas most failures observed with sandblasted surfaces were adhesive | MEDAL |
serum myoglobin by radioimmunoassay and creatine kinase were measured for up to days in patients following surgical procedures including total hip replacement and BL subcostal abdominal incisions SS myoglobin reached a maximum of micrograms litre median micrograms litre for major surgery patients on the first postoperative day but levels were still elevated by day in some patients creatine kinase reached a maximum of iu litre at day median iu litre for major surgery patients generally peaking day after myoglobin in individual patients these values may have significance when investigating a suspicion of coincident perioperative events such as myocardial infarction or malignant hyperthermia | MEDAL |
urban schools and schoolbased PHCs sbhcs in lowincome minority communities may be important points of MI for overweight and obese youth to date little is known about the interface of overweight youth and the public health system through sbhcs the objective of this study is to determine the prevalence geographic distribution sociodemographic and comorbidity factors associated with obese status as a part of a public health system needs assessment we conducted a crosssectional clustered sampling utilizing prospective anthropometric measurement and chart review demographic anthropometric and medical comorbidity data were collected from students in sbhcs in baltimore md usa students were geocoded to their primary residential address and assigned to a census block group using mapinfo v demographic and comorbidity associations were analyzed using multivariable LR analysis overall the mean body mass index bmi was sd and prevalence of obesity bmi th percentile and overweight bmi thth percentile was and respectively obesity was distributed among all the schools without one school being significantly more affected than others obese status was associated with gender poverty and several medical comorbidities such as asthma high blood pressure and disordered eating public health practitioners in this sbhc system appear to be faced with a greater burden of obesity than predicted by centers for disease control and prevention estimates given the ongoing interface with affected youth these schools and PHCs may be well situated to deliver public health obesity interventions | MEDAL |
PDs may affect comorbidity and health care utilization in subjects with panic attacks the purpose of this study was to identify differences in illness attitudes and behaviors and in coping strategies in communitybased subjects with panic attacks compared with controls a communitybased sample of subjects meeting dsmiiir criteria and matched controls completed the illness SMB questionnaire the illness attitude scale and the ways of coping checklist the panic group reported less healthy attitudes and behaviors although there were no paniccontrol differences in use of positive coping strategies the panic group reported more use of negative coping strategies the differences in illness attitudes and behaviors and in coping strategies may explain comorbidity and careseeking SMB in subjects with panic attacks | MEDAL |
dural arteriovenous fistula of the anterior condylar confluence accdavf is a rare AT1 of davfs that occurs around the HG canal transvenous embolization tve with coils has been performed for most accdavfs with a high clinical cure rate however some reports call attention to hypoglossal nerve palsy associated with tve due to coil mass compression of the HG nerve caused by coil deviation from the acc to the anterior condylar vein acv herein we report a case of accdavf in which an intraoperative conebeam computed tomography ct contributed to avoiding hypoglossal nerve palsy | MEDAL |
when nurses understand what interventions are needed to achieve desired patient outcomes they can more easily define their practice work complexity assessment wca is a process that helps nurses to identify interventions performed on a routine basis for their TPS patient population this article describes the wca process and links it to the nursing interventions classification nic and the nursing outcomes classification noc wca nic and noc are all tools that help nurses understand the work they do and the outcomes they achieve and that thereby acknowledge and validate nursings contribution to patient care | MEDAL |
percutaneous transluminal balloon valvuloplasty for MS represents an alternative method of treatment to standard surgical procedures of open or closed commissurotomy as well as valve replacement in this overview our results will be reported with respect to derivation of a summary of indications and contraindications for the procedure valvuloplasty for mitral stenosis was carried out in patients mean age years women and men in of the patients surgical procedures had been performed previously including an open or closed commissurotomy or isolated AV replacement nine patients were in nyha class ii in class iii and three in class iv markedly impaired motion of the valve and calcification was present in patients in patients the valve motion was relatively good and associated with mild changes in the subvalvular apparatus in and marked changes in in patients the valvuloplasty was carried out with a combination of two balloon catheters one x mm trefoil catheter and a singleballoon catheter of mm n or mm n diameter both catheters were inserted via the right FV the procedure required an average of one and onehalf hours before and after valvuloplasty CR RA heart catheterization with oxymetric determinations were carried out to detect possible shunts at the atrial level and LVG for PCD of MR was performed in addition to echocardiography and doppler examinations with continuous pulsedwave and color doppler studiesabstract truncated at words | MEDAL |
semidwarfing genes have been widely used in spring barley hordeum vulgare l breeding programs in many parts of the world but the success in developing barley cultivars with semidwarfing genes has been limited in north america exploiting new semidwarfing genes may help in solving this dilemma a RIL population was developed by crossing zau a semidwarf cultivar from china to nd a tall breeding L1 from north dakota to identify quantitative trait loci qtl controlling plant height a linkage map comprised of molecular markers was constructed simple interval mapping was performed for each of the eight environments a consistent qtl for plant height was found on chromosome hl this qtl is not associated with maturity and rachis internode length we suggest the provisional name qphh for this qtl qphh from zau reduced plant height to about of normal thus qphh is considered a semidwarfing gene other qtls for plant height were found but their expression was variable across the eight environments tested | MEDAL |
escapin is an lamino acid oxidase that acts on lysine to produce hydrogen peroxide ho ammonia and equilibrium mixtures of several organic acids collectively called escapin intermediate products eip previous work showed that the combination of synthetic eip and ho functions synergistically as an antimicrobial toward diverse planktonic bacteria we initiated the present study to investigate how the combination of eip and ho affected bacterial biofilms using pseudomonas aeruginosa as a model specifically we examined concentrations of eip and ho that inhibited biofilm formation or fostered disruption of established biofilms highthroughput assays of biofilm formation using microtiter plates and crystal violet staining showed a significant effect from pairing eip and ho resulting in inhibition of biofilm formation relative to biofilm formation in untreated controls or with eip or ho CT similarly flow cell analysis and confocal laser scanning microscopy revealed that the eip and ho combination reduced the biomass of established biofilms relative to that of the controls area layer analysis of biofilms posttreatment indicated that disruption of biomass occurs down to the substratum only nanomolar to micromolar concentrations of eip and ho were required to impact biofilm formation or disruption and these concentrations are significantly lower than those causing bactericidal effects on planktonic bacteria micromolar concentrations of eip and ho combined enhanced p aeruginosa swimming motility compared to the effect of either eip or ho alone collectively our results suggest that the combination of eip and ho may affect biofilms by interfering with bacterial attachment and destabilizing the biofilm matrix | MEDAL |
although the TRP vanilloid type trpvcontaining afferent nerve SF are widely distributed in the heart the relationship between trpv CF and cardiac ICM preconditioning pc has not been well defined using trpv knockout mice trpv we studied the role of trpv in pcinduced myocardial protection hearts of genetargeted trpvnull mutant trpv or wildtype wt mice were langendorffly perfused in the presence or absence of cgrp a selective CGRP-LI cgrp receptor antagonist or rp a selective neurokinin receptor antagonist when NF were subjected to three min periods of ischemia pc followed by min of GI and min of reperfusion ir pc before ir decreased left ventricular lv EDV pressure and increased lv developed pressure CF cf peakpositive maximum rate of rise of lv pressure in wt mice pcwt compared with pctrpv trpv or wt NF p and pc also decreased lv enddiastolic pressure in pctrpv compared with trpv cgrp or rp abolished pcinduced protection in wt but not trpv NF p moreover pc decreased LDH and infarct size in pcwt compared with pctrpv trpv or wt hearts and it also lowered these parameters in pctrpv compared with trpv hearts p radioimmunoassay showed that the release of substance p and cgrp T3 pc was higher in wt NF than in trpv hearts p which was attenuated by capsazepine in wt but not trpv NF thus pcinduced protection of the heart was impaired in trpv NF indicating that trpv contributes to the beneficial effects of preconditioning against ir injury through PR substance p and cgrp | MEDAL |
the mechanism of action underlying the beneficial effect of ifnbeta in multiple sclerosis ms is not understood to date little information is available on the effects of ifnbeta in experimental autoimmune encephalomyelitis eae the animal correlate of the human disease ms therefore we investigated the effects of recombinant rat ifnbeta rrifnbeta on eae in lewis rats with emphasis on a treatment regimen during the paralytic phase of the disease the results indicated that rrifnbeta dosedependently inhibited SLEDAI with CR prevention at a sc dose of uday provided that treatment was continued for wk discontinuation of treatment on day postimmunization resulted in a protracted and relapsing disease course with strongly enhanced clinical severity detailed IH of CNS system cns tissue of protected animals revealed an almost CR absence of cns lesions and a reduction in the number of infiltrating leukocytes accordingly isolation of mononuclear cells from spinal cord tissue of successfully treated eae rats revealed a reduction of approximately in the number of cells that produce ifngamma in response to the EP mbp furthermore rrifnbeta significantly enhanced SS corticosterone C2 which showed an inverse relationship with disease activity we show that rrifnbeta can have both beneficial and detrimental effects on SLEDAI dependent on the timing and the duration of treatment beneficial effects on eae are associated with inhibition of the extravasation of bloodderived mononuclear cells in the cns | MEDAL |
the vector requirements and course of larval development for tetrapetalonema llewellyni price a common filaria of raccoons in louisiana are described for the first time OD from microfilaria to L3 stage took place in the thoracic muscles of the biting midge culicoides hollensis melander and brues foote and pratt and required days under laboratory conditions the findings of this T0 suggest that species of culicoides may serve as NK vectors of t llewellyni | MEDAL |
there has been a steady increase in the prevalence of adolescent hypertension in recent years in order to prevent target organ damages it is important to determine the group of hypertensive adolescents if repeatedly elevated blood pressure values are observed with special emphasis on white coat hypertension which is particularly frequent at this age ambulatory blood pressure monitoring is highly recommended before pharmacological treatment is started in addition performing ambulatory blood pressure monitoring is recommended with target organ damage resistance to therapy and suspicion of SH the results of the widely available simpletouse device are easy to reproduce | MEDAL |
a field trial was performed under commercial feedlot conditions in western canada to compare the tau of a new formulation of LA OTC la to a standard LA OTC formulation la and florfenicol flor for the treatment of undifferentiated fever uf in calves that received metaphylactic tilmicosin upon arrival at the feedlot seven hundred and ninetyseven recently weaned auction market derived crossbred beef calves suffering from uf were allocated to of exp groups as follows la which received IM LA oxytetracycline mgml formulation at the rate of mgkg body weight bw at the time of allocation la which received IM longacting oxytetracycline mgml formulation at the rate of mgkg bw at the time of allocation or flor which received intramuscular florfenicol po at the rate of mgkg bw at the time of allocation and again hours later two hundred and sixtysix animals were allocated to the la group animals were allocated to the la group and animals were allocated to the flor group the relative efficacy of the la group as compared with the la and flor groups was assessed by comparing relapse chronicity wastage and mortality rates the overall mortality rr rate in the la group was significantly p lower than in the la group however the overall chronicity rr and overall wastage rr rates of the la group were significantly p higher than in the la group there were no significant p or differences in uf relapse rates or cause specific mortality rates between the la and la groups in tttempt to manage atrial fibrillation have been developed the purposes of this review are to present an overview of atrial fibrillation in patients with HF and to discuss the prevalence prognostic significance complications mechanisms and trials that have formed the therapies presently used | MEDAL |
cognitivebehavioural interventions combined with pharmacotherapy have gained importance in the treatment of bipolar disorders based on the vulnerabilitystress instability model psychoeducational cognitivebehavioural interpersonal and family interventions are provided this paper gives an overview of treatment programmes and their foci they include providing knowledge about the illness and its treatment modifying dysfunctional cognitions and symptom management as well as relapse prevention by promoting a SD life style there are several english and germanbased randomised controlled followup studies in englishspeaking countries which provide support for the efficacy of combined treatment since germanbased manualised treatment programmes have become available and have been shown to be clinically feasible and promising | MEDAL |
we report on a liveborn infant with mosaicism of tetraploidy and trisomy who had craniofacial CA cardiac and genitourinary defects agenesis of the corpus callosum and anomalies of limbs the infant died at age weeks molecular studies were done on peripheral BL and cultured amniocytes to determine the origin of the cytogenetic abnormalities on the basis of the results we describe a possible mechanism to explain these CA to our knowledge this infant represents the first reported case of mosaic trisomy with a tetraploid cell line | MEDAL |
the olfactory system specialized in the detection integration and processing of chemical molecules is likely the most thoroughly studied sensory system however there is piling evidence that olfaction is not solely limited to chemical sensitivity but also includes temperature sensitivity premetamorphic xenopus laevis are translucent animals with protruding nasal cavities deprived of the cribriform plate separating the nose and the olfactory bulb these characteristics make them well suited for studying olfaction and particularly thermosensitivity the present article describes the complete procedure for measuring temperature responses in the olfactory bulb of x laevis larvae firstly the electroporation of olfactory receptor neurons orns is performed with spectrally distinct dyes loaded into the nasal cavities in order to stain their axon terminals in the bulbar neuropil the differential IF between left and right receptor neurons serves to identify the γglomerulus as the only structure innervated by contralateral presynaptic afferents secondly the electroporation is combined with focal bolus loading in the olfactory bulb in order to stain mitral cells and their dendrites the d brain volume is then scanned under lineillumination microscopy for the acquisition of fast calcium imaging data while small temperature drops are induced at the OE lastly the postacquisition analysis allows the morphological reconstruction of the ts network comprising the γglomerulus and its innervating mitral cells based on TPS temperatureinduced ca traces using chemical odorants as stimuli in addition to temperature jumps enables the comparison between thermosensitive and chemosensitive networks in the olfactory bulb | MEDAL |
from january to december previously untreated patients with stage ii carcinoma of the uterine cervix were treated by RT in nine departments of radiotherapy in france this retrospective analysis was undertaken in an attempt to evaluate the therapeutic results and prognostically significant AF the initial clinical staging and the therapeutic guidelines were as outlined at the ut m d anderson hospital in houston all our patients were treated by standardized protocols combining external beam irradiation and intracavitary irradiation with cesium sources the overall LRC rate was with total disease control of uncorrected actuarial survival rates are at years and at years the incidence of severe posttherapeutic complications is clinical substaging patients age at the time of the diagnosis lymphangiogram findings and tolerance to external irradiation were all found to have prognostic significance according to those findings the possibilities of improving the results are discussed | MEDAL |
since the american cancer society illinois division has published end results of major cancer CS drawn from patient data contributed voluntarily by hospital CRs throughout the state the current T0 was undertaken in part to apprehend information regarding contested areas in the management of patients having differentiated papillaryfollicular TC a total of patients with either papillary or follicular carcinoma of the thyroid from different illinois hospitals and providing years of followup information lifetable analysis were retrospectively analyzed for demographic disease and treatmentrelated predictors of survival MVA using the cox PH method was made for stage age race sex morphology history of radiation SE presence of positive CL NO initial surgical treatment postoperative iodine therapy and replacementsuppressive thyroid hormone treatment statistically significant p less than or equal to predictors of favorable survival T3 TC were low stage i and ii young age less than years white race female sex and the administration postoperatively of either thyroid hormone or radioactive iodine factors that had no influence on survival were CL node status choice of initial surgical treatment and a PH of prior irradiation we suggest that where a prospective clinical trial is impracticable a retrospective analysis of a C1 and detailed database such as that available from cooperating hospitalbased RT registries may yet provide useful insights to solutions of CA management problems | MEDAL |
an established rat intracranial glioma was successfully treated through the tumoricidal bystander effect generated by intratumoral i.t. of RBM stromal cells bmscs transduced with the herpes simplex virusthymidine kinase gene bmsctk cells followed by systemic ganciclovir administration in the present T0 we tested the bystander effect of this treatment strategy when using human bmscs as the vector cells human bmsctk cells were mixed with various kinds of BB tumor cell lines human and rat glioma cells and examined in vitro and in vivo tumoricidal bystander effects by coculture T0 and coimplantation T0 in the nude mouse respectively a significant in vitro bystander effect was observed between human bmsctk cells and any of the tumor cells examined in the ganciclovircontaining medium a potent in vivo bystander effect against human and C6 cells was also demonstrated when ganciclovir was administered migratory activity of the human bmscs toward the tumor cells was enhanced by the CS media obtained from both human and rat glioma cells compared to the fresh media the results of this study have demonstrated that the bystander effect generated by bmsctk cells and ganciclovir is not cell typespecific suggesting that the strategy would be quite feasible for clinical use | MEDAL |
the argininerich fraction of CT histone histone b exerts bactericidal activity on various coliform bacilli and micrococci under certain conditions in vitro final concentrations of less than microg histone per ml kill susceptible microbes without detectable morphological alteration or lysis among the microorganisms highly susceptible to histone are escherichia salmonella shigella pseudomonas klebsiella and micrococcus pyogenes var albus less susceptible or completely resistant are proteus serratia micrococcus pyogenes var aureus and various types of hemolytic streptococci coliforms grown on solid media are much more resistant to the lethal effect of histone than are those cultured in liquid media this difference is apparently related to the physiological state of the bacteria agar grown microorganisms washed with water remain resistant to histone whereas incubation in BB rapidly renders them more susceptible histone is adsorbed onto heatkilled e coli k under conditions suitable for lethal action on this organism the bactericidal activity of histone is but little affected by ph of the test system but ionic strength of the medium exerts a marked influence the lethal action being reduced or blocked as the salt concentration reaches levels higher than that of m nacl relatively high concentrations of rb serum or of bovine plasma albumin reduce the bactericidal activity of histone in a medium at ph these SS preparations are however essentially without effect in the test system at ph the bactericidal effect of histone is antagonized by addition to the medium of small amounts of certain basic substances protamine spermine or of various acid PSs heparin nucleic acid LPS the rate of killing of e coli k by histone increases as the temperature and the concentration of histone are raised within the limits studied this rate also appears to be directly proportional to the concentration of bacteria in the system | MEDAL |
the aim of present T0 was to verify the in vitro antitumor activity of a ruthenium complex cisdichlorotetraamminerutheniumiii chloride cisruclnhcl toward different RT cell lines the antitumor studies showed that rutheniumiii complex presents a relevant cytotoxic activity against murine b cell lymphoma a murine ascitic sarcoma s human breast adenocarcinoma skbr and human t cell leukemia jurkat cell lines and a very low cytotoxicity toward human peripheral blood MNC cells the rutheniumiii complex decreased the fraction of RT cells in gg andor gm phases indicating that this compound may act on restingearly entering gg cells andor precycling gm cells the cytotoxic activity of a high concentration mg ml of cisruclnhcl toward jurkat cells correlated with an increased number of annexin vpositive cells and also the presence of dna fragmentation suggesting that this compound induces apoptosis in RT cells the development of new antineoplastic medications demands adequate knowledge in order to avoid inefficient or toxic treatments thus a mechanistic understanding of how metal complexes achieve their activities is crucial to their clinical success and to the rational design of new EDC with improved potency | MEDAL |
we present a case of insufficient BL femoral subtrochanteric fractures in a patient who was treated with imatinib mesylate an anticancer drug for year T3 a diagnosis of CML-BC cml a yearold woman presented with bilateral thigh pain for months a plain radiograph revealed BL psis of astaxanthin and its carotenoid precursors from xanthophyllomyces dendrorhous the hplc method is accomplished by employing a c column and the mobile phase methanolwateracetonitrile dichloromethane vvvv astaxanthin is quantified by PCD at nm the carotenoid precursors are identified by lcapcims and uvvis absorption spectra peaks showed in the hplc chromatogram are identified as carotenoids in the monocyclic biosynthetic pathway or their derivatives in the monocyclic carotenoid pathway dihydroxybetapsicarotenedione dcd is produced through gammacarotene and torulene | MEDAL |
desmoplastic malignant melanoma dmm is a rare variant of spindle cell melanoma we report a case of dmm on the forehead secondarily involving the orbit the diagnosis was based on LM features including prominent peripheral cell nest formation and spindle cell fascicles in densely collagenous stroma immunohistochemical studies showed strong uniform staining for s antigen throughout the tumour it was negative for hmb smooth SMA desmin cytokeratins and type iv collagen electron microscopy showed neither melanosomes nor myelin figures the clinical and histological characteristics of DMM and its differential diagnosis of malignant schwannoma are discussed dmm has a poor prognosis since it tends to invade deeply recur locally and metastasize readily | MEDAL |
erosive hand OA is common and debilitating diagnosis is based on the presence of bone erosions which can appear late ultrasonography allows earlier diagnosis the presence of apatite deposits could be of poor prognosis non pharmacological treatment includes the explanation of the inflammatory phenomena involved and the use of splints and PCS therapy drug therapy includes analgesics nsaids and infiltration of a steroid chondroitin sulfates have an analgesic and functional effect proven dmards such as hydroxychloroquine and methotrexate have been used successfully some patients also benefited from isotope synoviortheses new therapeutic ways based on the pathophysiology of the disease are new under DUE | MEDAL |
pseudopregnancy pp in the rat is characterized by maintenance of the corpora lutea and twice daily surges of prolactin at h and h four days T3 pp was induced by CS with a glass rod rats were cannulated via the right carotid artery and one day later received an implant of ovine prolactin mg or Al controls into the ME of the hypothalamus fortyfive of rats implanted with prolactin showed a termination of pp within days ova were recovered from of these rats whereas all of the controls remained pseudopregnant and did not ovulate ovarian and uterine weights were increased in rats implanted with prolactin plasma PRL in the rats implanted with prolactin were lower each day at and h compared to controls plasma lh C2 rose gradually T3 prolactin implantation and in those rats in which ovulation occurred a preovulatory lh rise was detected at h on the previous day it is concluded that daily surges of prolactin play an essential role in maintaining pp in the rat and that implantation of prolactin into the ME results in inhibition of prolactin transfer factor tf in an attempt to improve their clinical condition before and during treatment cellular reactivity was followed in vivo delayed type skin CR and in vitro lymphocyte transformation in all patients positive skin reactions were occasionally observed during tf treatment the lymphocyte CR in vitro to phytohaemagglutinin pha was slightly diminished the responding capacity of the mixed lymphocyte SC mlc severely impaired and the response to bacterial viral and fungal antigens absent before and during tf treatment no clinical improvement was observed after large doses of tf no rho between skin test reversal and tf administration was found and lymphocyte transformation in vitro did not improve subsequently a double blind trial in which we compared the effect of tf and placebo was carried out in these patients which revealed no effect of tf therapy it is concluded that the results of treatment with tf in these patients are highly controversial | MEDAL |
an CA for the detection and quantitation of immune complexes is described exp immune complexes or aggregated human gamma globulin ahg were incubated with polymorphonuclear WBCs pmn after challenge of the pmn with OPZ CL was recorded in a scintillation spectrometer a quantitative inhibition of CL could be demonstrated by the interaction of pmn with immune complexes or ahg exp immune complexes of bovine SS albuminantibovine serum albumin were formed and tested by this CA and imm m addition of the precursors shikimate and dihydroxybenzoate to strain ab arob and dihydroxybenzoate to strain an enta allowing these strains to make enterochelin resulted in an increase in fe uptake and a corresponding sharp increase in killing by streptonigrin addition of enterochelin itself m caused an even more pronounced effect studies on the effect of citrate in strain an fep showed that this mutant was not killed by streptonigrin x m even in the presence of citrate however overnight growth in citrate induced fe uptake by means of the ferric citrate transport system and resulted in killing by streptonigrin these studies showed a clear correlation between the NC in C2 of intracellular iron and the bactericidal effectiveness of SN | MEDAL |
we report a case in which pulsed wave doppler echocardiography and color flow imaging of blood flow direction in an anomalous coronary i.a. from the pulmonary artery assisted in the correct diagnosis and confirmed the adequacy of the surgical correction lowvelocity color scales were used to show retrograde filling of the left coronary artery before surgery and antegrade filling of the left PD T3 surgery detecting direction of blood flow in CAs should increase the accuracy of the noninvasive diagnosis of anomalous LCA from the pulmonary artery | MEDAL |
the micellization SMB of hybrid dendriticstar copolymers with solvophilic dendritic units is studied by means of brownian dynamics simulations the critical micelle concentration and the micelle size and shape are examined for different solvophobicsolvophilic ratios r as a CF of the number of the dendritic and linear arms hybrid dendriticstar copolymers with one dendritic and up to three solvophobic linear branches form spherical micelles with preferential aggregation number those with two dendritic arms and three solvophobic branches form micelles with wide aggregation numbers only for small values of r for hybrid dendriticstar copolymers with three dendritic arms and two or three solvophobic linear arms micelles with wide aggregation numbers are also formed but for slightly higher values of r our results for the aggregation number are compared with existing results of other architectures obtained at the same temperature and an inequality for the aggregation number is proposed | MEDAL |
limited english proficiency lep is associated with poor health status and worse outcomes | MEDAL |
the aim of this study is to investigate in vitro interactions between hydrophobic acrylate IOL iols and their biological environment the influence of lens chemical composition and aging on fibronectin fn adsorption and on iols cytotoxicity on human lens epithelial cells was examined cytotoxicity of acrylate mono for screening adhesion characteristics of pathogens to ligandmodified interfaces | MEDAL |
stimulated brillouin backscattering of light is shown to be drastically enhanced in electronpositron plasmas in contrast to the suppression of stimulated raman scattering a generalized theory of threewave coupling between EM and plasma waves in twospecies plasmas with arbitrary mass ratios confirmed with a comprehensive set of PIC simulations reveals violations of commonly held assumptions about the SMB of electronpositron plasmas specifically in the electronpositron limit threewave parametric interaction between CS and the plasma acoustic wave can occur and the acoustic wave phase velocity differs from its usually assumed value | MEDAL |
HIV virus hiv env glycoprotein is synthesized as a polyprotein precursor of kda gp and is subsequently cleaved into an amino terminus subunit gp and a CT transmembrane subunit gp two synthetic peptides corresponding to CAA sequences and respectively as deduced from the nucleotide CS of htlviiib gp were synthesized and used to assess their effects on normal human lymphocyte blastogenesis MMP and conjugated to protein carriers but not free MMP exerted a pronounced suppression of the normal human LP response to concanavalin a con a phytohemagglutinin pha pokeweed mitogen pwm and alloantigens a synthetic peptide homologous to a CAA sequence of the gene product of hiv transacting transcriptional tat iii gene had no suppressive effects MMP and also inhibited the ildependent proliferation of the mu ctll cell L1 and the phainduced proliferation of normal mouse spleen cells hiv peptideinduced suppression of human blastogenesis required a to day incubation of responder cells with peptides suggesting that binding of MMP to the CM was not sufficient for suppression these results suggest that in addition to the selective cytopathic effects of hiv the etiological agent of the AIDS syndrome aids on the thelperinducer lymphocyte subset viral peptidemediated IS may also play an important role in the pathogenesis of the disease moreover these data clearly indicate the need to address the potential immunosuppressive property of hiv antigens in the effort to select and develop effective prophylactic means against aids | MEDAL |
the aim of this T0 was to examine the results of GK radiosurgery for patients with residualrecurrent or newly diagnosed solid hemangioblastomas the patients had solid hemangioblastomas and all patients underwent gamma knife RS seven patients had von hippellindau disease and six had sporadic disease when individual lesions were considered the overall mean dose at the RT periphery was gy range gy and the average maximum RT dose was gy range gy the mean duration of followup with mri was months at the last followup DUE growth control was achieved for all tumors partial remission in three tumors and no NC in tumors no radiationrelated complications were encountered our findings reinforce the view that gamma knife RS is ERP and safe for the management of solid hemangioblastomas with a diameter less than cm whether they are sporadic or associated with von hippellindau disease the high response rate and lack of any radiationinduced sideeffects confirms the suitability of the doses used in the present T0 | MEDAL |
while germline mutations in the adenomatous polyposis coli apc gene cause the hereditary colon CA syndrome familial adenomatous polyposis fap the role of common germline apc variants in sporadic adenomatous polyposis remains unclear we studied the association of eight apc single nucleotide polymorphisms snps possibly associated with functional consequences and previously identified geneenvironment dietary fat intake and HRT hrt use interactions in relation to T3 colorectal adenoma in cases and sex and racematched controls randomly selected from the screening arm of the prostate lung colorectal and OC screening trial cases had at least one verified advanced adenoma of the distal colon controls a negative sigmoidoscopy we did not observe an association between genotypes for any of the eight apc snps and T3 distal adenoma risk pglobal genebased frequencies of identified common haplotypes did not differ between cases and controls pglobal haplotype test however the risk for T3 distal adenoma was threefold higher for one rare haplotype cases controls odds ratio or confidence interval ci the genetic association between dv and T3 distal adenoma was confined to persons consuming a highfat diet pinteraction similar interactions were not observed with hrt use in our large nested casecontrol T0 of T3 distal adenoma and clinically verified adenomafree controls we observed no association between TPS apc snps and advanced adenoma fat intake modified the apc dvadenoma association but further studies are warranted | MEDAL |
CA chemoprevention is a new challenging issue for oncology for breast cancer different strategies for risk avoidance chemopreventive measures and chemosuppressive interventions are being investigated attention is presently focused on two compounds the synthetic retinoid fenretinide hpr and the antioestrogen tamoxifen and their possible synergism more data are now available on the expression and regulation of retinoic acid receptors in human BCCs and on the consequences of the interaction of retinoids with plasma retinol binding protein mechanistic relationships between retinoids and growth AF like transforming growth factorbeta have been demonstrated in the frame of a central RII system of the state of differentiation and proliferation in which tamoxifen is involved as well recent data from different trials using tamoxifen as adjuvant treatment have shown a decrease in the incidence of contralateral new primaries and that it could soon become unethical not to prescribe this drug to all nodenegative oestrogen receptorpositive PM patients | MEDAL |
aging is due to the accumulation of damage over time that affects the CF and survival of the organism however it has proven difficult to infer the relative importance of the many processes that contribute to aging to address this here we outline an RPA that may prove useful in analyzing aging in this RPA the function of the organism is described as a set of interacting physiological systems degradation of their outputs leads to functional decline and death as a result of aging in turn degradation of the system outputs is attributable to changes at the next hierarchical level down the cell through changes in cell number or function which are in turn a consequence of the metabolic history of the cell within this framework we then adapt the methods of metabolic control analysis mca to determine which modifications are important for aging this combination of a hierarchical framework and the methodologies of mca may prove useful both for thinking about aging and for analyzing it experimentally | MEDAL |
the report is based on the observation of patients with PD syndromes treated with ldopa with inhibitor including patients treated during years it was found that treatment with ldopa with the inhibitor gave good or very good results in most patients with these syndromes with a relatively good drug tolerance during the first three months with passing time the effect of the drug on the manifestations of the disease decreases and the tolerance becomes aver worse the results of the treatment are worse although the applied daily dose is increased by a metan value of at the same time the frequency of such complications as hyperkineses anxiety and depression labyrinthinecerebellar signs increases urinary passage disturbances and mental Kd develop and their temporal association with LD treatment is unquestionable while a causeandeffect rho seems likely in the conclusion the author suggests the need for a careful consideration of each decision of treatment with LD and the possibility of improving the results of parkinsonism treatment is seen in the production of new optimal forms of he already present drugs monitoring blood drug C2 and practical utilization of the synergism between the group of drugs stimulating the dopaminergic system and the group of drugs inhibiting the acetylcholinergic system | MEDAL |
in urethaneanesthetized rats a process termed maximal dentate activation has been shown to be associated with the lengthening of afterdischarges that occurs with repeated hippocampal stimulation maximal dentate activation is a unique paroxysmal form of EDs consisting of large amplitude population spikes in the dentate gyrus the current experiments examined the relationship of maximal dentate activation to kindling of motor seizures in the awake animal both long duration or s and short duration s stimulus trains in either the hippocampus or the amygdala were effective in eliciting maximal dentate activation repeated stimulation of the amygdala produced lengthening of afterdischarges and kindling of motor responses but only after maximal dentate activation had appeared in response to the CS- over the course of amygdala kindling the duration of maximal dentate activation lengthened with increasing severity of behavioral seizures this evidence supports the hypothesis that maximal dentate activation is a marker for the presence of seizures distributed throughout limbic circuits in addition the data suggest that maximal dentate activation may be an important process involved in the acquisition of kindled responses | MEDAL |
we present a middleaged woman complaining of weakness lethargy and WL for months positron emission tomography petct scan revealed huge bilateral adrenal masses with intense ffluorodeoxyglucose avidity biopsy and immunohistochemical IF were compatible with mantle FCCL mcl the patient received six cycles of RTX cyclophosphamide doxorubicin vincristine and prednisone rchop followed by four cycles of intrathechal methotrexate chemotherapy bone marrow aspiration biopsy was performed for initial staging and also before bone marrow transplantation followup petct scan T3 completion of chemotherapy and before bone marrow transplantation demonstrated complete metabolic response with no evidence of abnormal metabolic activity in either adrenal gland or bone marrow mcl has an aggressive nature and is usually considered incurable however there have been a few reports indicating favourable treatment response when mcl is rarely arising from the adrenal PG dramatic response of a primary adrenal mcl to rchop is documented in this case | MEDAL |
to elucidate the role of innate immunity in susceptibility to the rat of multiple sclerosis EAE eae we induced eae by immunization with spinal cord homogenate sch plus complete freund adjuvant or carbonyl iron in inbred rat strains lewis are considered susceptible pvgcrta pvg as semisusceptible and brown norway bn as resistant to eae immunization with schcarbonyl iron resulted in clinical disease in all strains but the pathologic features of eae in the resistant bn and the semisusceptible pvg rats differed from those in the Le and pvg MM of eae induced with schcomplete freund adjuvant in bn and pvg rats there were numerous inflammatory lesions with prominent involvement of microglia and to a lesser extent perivascular macrophages these data suggest that different C2 of activation of the innate immune system by different adjuvants determine whether eae will or will not develop accordingly the widely accepted scale of susceptibility to eae OD Le pvg bn should be revised because it does not take into account the important contribution of the composition of the adjuvant to the quality and quantity of the innate immune response and consequently to the generation and extent of the pathogenic tcellmediated that is adaptive autoimmune disease | MEDAL |
mixedmode chromatography uses a multimodal functional resin mainly composed of electrostatic and aromatichydrophobic CG here we have tested mixedmode resins anionexchange capto adhere and cationexchange capto mmc using model proteins ie an fcfusion etanercept and bovine SS albumin bsa when etanercept was produced in chinese hamster ovary cells a C1 amount of misfolded species was generated a novel technology to achieve ERP separation of the misfolded or aggregated species has been developed in this study using these mixedmode columns and elution conditions that combine ph change and nacl or arginine at different concentrations etanercept which has been purified by proteina chromatography was bound to the capto mmc or capto adhere columns under various conditions and eluted by modulating the ph and salt or arginine concentration the misfolded species occurred in the fractions at higher salt or Arg concentrations most likely reflecting stronger electrostatic and hydrophobic interactions of the misfolded species with these mixedmode resins another MM protein bsa containing several oligomeric species was also subjected to capto adhere or capto mmc chromatography using either nacl or Arg gradient elution with a greater recovery by arginine gradient the oligomers were effectively separated on these mixedmode columns using either gradient elution eluting in later fractions similar to etanercept misfolded species | MEDAL |
the most common site for the occurrence of intubationinduced tracheal damage is at the area in contact with the inflatable cuff after the NC from highpressure to LP cuffs L1 tracheal lesions still continue to occur this is a case of tracheal stenosis that occurred T3 days of intubation with standard cuffed tube whose cuff pressure was assessed by subjective means three weeks later patient was in need of reintubation the tr was found to be stenotic at the site of the previous tube cuff emergenar rearrangements underlying even and oddnumbered steps must differ but the mechanism by which such rearrangements lead to limping remains unsolved here we used an optical force clamp to measure individual recombinant dimers and test candidate explanations for limping introducing a covalent crosslink into the stalk region near the heads had no effect on limping ruling out possible stalk misregistration during coiledcoil formation as a cause limping was equally unaffected by mutations that produced fold changes in stalk stiffness ruling out models where limping arises from an asymmetry in torsional strain however limping was enhanced by perturbations that increased the vertical component of load on the motor including increases in bead size or net load and decreases in the stalk length these results suggest that kinesin heads take different vertical trajectories during alternate steps and that the rates for these motions are differentially sensitive to load | MEDAL |
imidazolinepreferring receptors are important in the pathophysiology of hypertension the selective lreceptor agonists are moxonidine cimetidine and rilmenidine some clinical studies indicate the usefulness of moxonidine therapy in hypertension arrhythmias and AIM | MEDAL |
the tau and tolerability of the selective reversible monoamine oxidase a inhibitor moclobemide mgday and the selective serotonin uptake inhibitor fluoxetine mgday were compared in a sixweek singlecentre doubleblind fixeddose study in patients n with double depression dsmiiir dysthymia with superimposed L1 depressive episode using weekly assessment on the hamilton depression rating scale hdrs items and clinical global impression cgi scale the primary tau outcome measure was a decrease or in end of treatment hdrs score secondary measures were the mean total endpoint hdrs scores and percentages of cgi very good and good responses tolerability was measured by the frequency and severity of volunteered AEs there were no significant differences in secondary efficacy outcome measures but more patients achieved a or decrease in hdrs score on moclobemide vs p the only adverse event was mild transient anxiety n with moclobemide the results suggest that moclobemide and fluoxetine are equally well tolerated and at least similar in efficacy in double depression evidence that moclobemide may be more effective requires confirmation in a larger comparative T0 incorporating a placebo control group | MEDAL |
a peroxidaseantiperoxidase pap technique was developed for the identification of haemophilus somnus bacteria in lung tissues of calves antisera raised against somatic and wall antigens of a danish and american strain of h somnus were produced experimentally INF murine tissues were used for the determination of the sensitivity and specificity of AS that had been heterologously absorbed with antigens of crossreacting bacteria ie pasteurella haemolytica and PM none of the antisera reacted with actinomyces pyogenes an antiserum raised sus median raphe ht projections in mediating these effects by investigating the impact of separate versus combined infusions of dht into these CS on drl responding adult male rats received i.v. of micrograms dht into both dorsal raphe and median raphe dorsal raphe only or median raphe only shamoperated controls received vehicle injections animals were then TR to respond on a drl s schedule for days combined dorsal raphe and MR dht infusions depleted striatal and HPC ht by increased responding decreased the number of reinforcers earned lowered the mean interresponse time irt and shifted the frequency distribution of irts to the left median raphe dht infusions induced similar behavioural effects and reduced hippocampal ht by dorsal raphe dht lesions that depleted striatal ht by and HPC ht by had no effect on behaviour in a final experiment it was shown that providing an external cue CS to signal food availability and that removed the need to rely on internal timing processes resulted in rapid acquisition of responding and prevented the behavioural deficits in dhtlesioned rats following removal of this cue responding deteriorated and the dht treated animals performed worse than controls these results suggest that destruction of ht neurons arising from the median raphe is sufficient to disrupt behaviour maintained by a drl s schedule and that the behavioural changes observed may involve a deficit in timing | MEDAL |
cyclosporine csa mol wt is a cyclic undecapeptide with potent IS properties which have made it extremely useful for preventing the rejection of Tx organs the drug has severe sideeffects howe good metabolic control using oral hypoglycaemic agents and insulin were studied before and T3 days of captopril at mgday we measured the following parameters blood pressure HR rate fructosamine and a daily profile for blood glucose and cpeptide there was a statistically significant reduction in systolic and diastolic blood pressure no difference was observed in the C2 of blood gl and fructosamine insulin secretion as determined by cpeptide levels was not modified in contrast with findings reported for the use of betablockers diuretics or nifedipine it seems that captopril is useful and without SE even in secondaryfailure diabetic patients characterized by unstable metabolic control | MEDAL |
dendritic cells dc form a specialized system for presenting ag to naive or quiescent t cells and consequently play a CE role in the induction of t and b cell immunity in this T0 we used dc generated from IP progenitors to analyze the effect of il on the accessory function of human dc we demonstrate that immature dc harvested on days to and exposed to il for the last days of SC show a strongly reduced capacity to stimulate a cd t cell response in an allogeneic mlr in a dosedependent manner in contrast fully mature dc are CR resistant to the effects of il these results were obtained in both an alloantigeninduced mlr and an anticd mabinduced response of primed and naive cdra cd t cells facs analysis revealed inhibition of the upregulation of the costimulatory molecules cd and cd and the TPS dc marker cd in dc pretreated with il these data suggest that il inhibited the development of fully mature dc furthermore dc precultured with il but not controls induced a state of alloantigenspecific anergy in cd TA and of peptidespecific anergy in the influenza hemagglutininspecific t cell clone ha analysis of the supernatants of these anergic TA revealed a reduced production of il and ifngamma compared with that in control cells collectively these data suggest that il converts immature dc into tolerogenic apc which might be a useful tool in the therapy of patients with autoimmune or allergic diseases | MEDAL |
ascending sensory information flowing through the visual thalamus is dynamically regulated by a number of modulatory influences an important part of this ascending modulation is a ACh projection from the PBR of the BS pbr in addition to containing cholinergic fibers this projection has been identified in some species as the exclusive extrinsic source of fibers containing the neuronal form of nitric oxide synthase nnos in this T0 we examined the nitrergic innervation to the AD mouse visual thalamus VA tracttracing with fluorescent microspheres was combined with nnos and choline acetyltransferase chat immunocytochemistry and nicotinamide adenine dinucleotide phosphate diaphorase nadphd histochemistry to identify the source of nitrergic innervation doublelabeling revealed that only two regions of the mouse brain contained nitrergic neurons that projected to the visual TH the pedunculopontine tegmentum and to a lesser extent the lateral dorsal tegmentum ldt though the ldt projected heavily to the mouse visual thalamus very few of the retrogradely labeled neurons in that region colocalized nadphd these observations suggest that the parabrachial brainstem region is the primary source of nitrergic fibers in the mouse visual thalamus similar to that found in cat and monkey such similarity suggests that the presence of nnos in presynaptic terminal fields of the visual thalamus is an important conserved property of thalamic physiology and that the mouse is a valid MM for studies of nnos functions in the brain | MEDAL |
the abilities of analogues of PEA and two analogues of oleoylethanolamide to affect the ca influx into human embryonic A6 expressing the human vanilloid receptor hvrhek cells in response to anandamide aea have been investigated using a flipr assay and a bovine serum albumincontaining CA medium only palmitoylethanolamide produced any effect in the absence of aea the ability of palmitoylethanolamide to potentiate the response to aea was retained when the nchchoh group was replaced by nchchclwhereas replacement with nalkyl substituents from h up to chch resulted either in a reduction or in a complete loss of this activity the tertiary NH nchch and nmorpholino analogues of palmitoylethanolamide potentiated the response to microm aea to a greater degree than the parent compound whereas the nch analogue was inactive and produced leftward shifts in the DR curve for aea activation of ca influx into hvrhek cells ec values for aea to produce ca influx into hvrhek cells were and microm in the presence of and microm respectively the corresponding values for were and microm respectively the compounds did not affect the DRCs to capsaicin the ability of oleoylethanolamide to potentiate aea is retained by the nchch and nchch analogues and respectively and produced a small approximately inhibition of the IB of hcp and hwin to cb and B6 receptors respectively expressed in cho cells the EDC inhibited the metabolism of microm haea by rat brain FAAH with ic values of and microm respectively in contrast and were without effect on either IB to cb receptors or fatty acid amidohydrolase activity minor reductions in the accumulation of microm haea into c glioma cells were seen at microm concentrations of and it is concluded that and selectively enhance aea effects upon vr receptors without potentially confounding effects upon cb receptors or FAAH activity | MEDAL |
incorporation of human foamy virus hfv envelope proteins into L1210 virus mulv particles was studied in a transient transfection packaging cell system we report here that wildtype hfv env protein can pseudotype mulv particles albeit at low efficiency complete or partial removal of the hfv cytoplasmic tail resulted in an abolishment or reduction of hfvmediated infectivity implicating a role of the hfv envelope CT in the pseudotyping of mulv particles mutation of the endoplasmic reticulum retention signal present in the hfv envelope cytoplasmic tail did not result in a higher relative infectivity of pseudotyped retroviral vectors however a chimeric env protein containing an unprocessed mulv envelope cytoplasmic domain fused to a truncated hfv env protein showed an enhanced hfv specific infectivity as a result of an increased incorporation of chimeric envelope proteins into mulv particles | MEDAL |
new imaging criteria for recent small subcortical infarcts have recently been proposed replacing the earlier term lacunar infarction but their applicability and impact on lesion selection is yet unknown | MEDAL |
this in vitro T0 evaluated the efficacy of MCT ct in marginal leakage detection of class v restorations standardized class v S9 with cervical margins in dentin and occlusal margins in enamel were made in extracted human molars and restored with dental bonding agents and resin composite all teeth were then immersed in ammoniacal silver nitrate solution for hours followed by a developing solution for eight hours each restoration was scanned by microct the depth of marginal silver leakage in the central scanning section was measured and the threedimensional images of the silver leakage around each restoration were reconstructed afterward all restorations were cut through the center and examined for leakage depth using a microscope the silver leakage depth of each restoration obtained by the microct and the microscope were compared for equivalency the silver leakage depth in C2 walls observed by microct and microscope showed no significant difference however in certain cases the judgment of leakage depth in the occlusal wall in microct image was affected by adjacent enamel structure providing less leakage depth than was observed with the TSM p microct displayed the threedimensional image of the leakage around the class v restorations with clear borders only in the dentin region it can be concluded that microct can detect nondestructively the leakage around a resin composite restoration in two and 3D with accuracy comparableng genes the major subclass corresponds to receptor and resistant homolog genes like nucleotidebinding site leucinerich repeat nbslrr ptolike rlks bger and rgha all not previously described in coffee rust responses the second major subclass included kinases where two mitogenactivated kinases mapk are identified further mRNA analysis was performed for selected genes by realtime pcr mRNA analysis at and h postinoculation the expression of genes involved in signaling and defense was higher at and h after inoculation respectively the nbslrr was the more differentially expressed gene between the signaling genes four times more expressed in the resistant TT and thraumatin pr was the more expressed between all genes six times more expressed multivariate analysis reinforces the significance of the temporal separation of identified signaling and defense genes early expression of signaling genes support the hypothesis that higher expression of the signaling components up regulates the defense genes identified additionally the increased mRNA of these two gene sets is associated with a single monogenic resistance trait to to leaf coffee rust in the interaction characterized here | MEDAL |
to assess the influence of different sampling methods on human amniotic fluid haf during metabonomics analysis and to establish a metabolite profile DB for normal human amniotic fluid four exp CG the group of FD of freezethawing of storage at degrees c and of keeping in RT and a control group were investigated by use of hnmr spectroscopy respectively the data of hnmr spectroscopy was treated by principal components analysis pca the results showed that by comparison with the control there were distinct differences in the exp groups except the group of storage at degrees c therefore it is possible to use hnmrbased metabonomics technique for analysis of haf moreover during the tests careful treatments of haf should be institued to minimize the influence on the samples | MEDAL |
chromatin remodelling events play an important role in the secondary metabolism of filamentous fungi previously we showed that a bacterium streptomyces rapamycinicus is able to reprogram the histonemodifying sptadagcnacetyltransferaseada sagaada complex of the model fungus aspergillus nidulans consequently the histone h amino acids lysine and lysine at distinct secondary metabolism genes were specifically acetylated during the bacterial fungal interaction which furthermore was associated with the activation of the otherwise silent orsellinic acid gene cluster to investigate the importance of the histone modifications for distinct mRNA profiles in fungal AA metabolism we exchanged several amino acids of histone h of a nidulans these amino acids included lysine residues and as well as serine and threonine lysine residues were replaced by arginine or glutamine residues and serinethreonine residues were replaced by alanine all generated mutant strains were viable allowing direct analysis of the consequences of missing posttranslational histone modifications in the mutant strains major changes in the expression patterns at both the transcriptional and metabolite C2 of the penicillin sterigmatocystin and orsellinic acid biosynthesis gene clusters were detected these effects were due mainly to the substitution of the acetylatable lysine of histone h and were enhanced in a lysine lysine double mutant of histone h taken together our findings show a causal linkage between the acetylation of lysine residue of histone h and the transcription and product formation of secondary metabolite gene clusters | MEDAL |
in select cases LP has been observed to be a paraneoplastic condition sometimes associated with paraneoplastic pemphigus a disease as ci and ci respectively the authors conclude that caribbeanborn women and usborn black women have disparate moderate rates but equivalent very low birth weight rates | MEDAL |
human platelets contain at least two forms of PH ST pst a thermolabile tl form for which dopamine is a ATP and a TS ts form for which micromolar concentrations of phenol can serve as ATP at higher concentrations phenol is also a ATP for the tl form studies of the regulation and the possible clinical value of measurements of PLT pst have been hampered because there is no TPS ATP for the ts form of the enzyme the purposes of these experiments were to determine whether there might be a better substrate than PH for use in measurement of the activity of the ts form of platelet pst and to attempt to physically separate the two forms of the PLT enzyme the results of ATP kinetic thermal stability and inhibitor studies performed with platelet homogenates were all compatible with the conclusion that pnitrophenol and ohmelatonin were substrates for both the ts and tl forms of platelet pst norepinephrine epinephrine and ohtryptamine were substrates for only the tl form the apparent km constants of the two forms of pst for pnitrophenol differed by fold when measured in platelet homogenates this difference was times greater than that which has been reported for PH therefore PNP is the preferred ATP for measurement of the ts pst activity if interference by the tl activity is to be avoided this information made it possible to use PNP as a substrate in experiments designed to separate the two forms of platelet pstabstract truncated at words | MEDAL |
the hydrophobic hpb nature of most polymeric biomaterials has been a major obstacle in using those materials in vivo due to low compatibility with cells however there is little knowledge of the molecular detail to explain how surface hydrophobicity affects cell responses in this study we compared the proliferation and apoptosis of human osteoblastic mg cells adhered to hydrophilic hpl and hydrophobic surfaces on the hydrophobic surface less formation of focal contacts and actin SFs a delay in cell cycle progression and an increase in apoptosis were observed by using FB growth factor fgf as a model growth factor we also investigated intracellular signaling pathways on hydrophilic and hydrophobic surfaces the activation of ras akt and erk by fgf was impaired in mg cells on the hydrophobic surface the overexpression of constitutively active form of ras and akt rescued those cells from apoptosis and recovered Tc progression furthermore their overexpression also restored the actin CSK organization on the hydrophobic surface finally the proliferative antiapoptotic and cytoskeletal effects of constitutively AS ras in mg cells on the hydrophobic surface were blocked by wortmannin and pd that inhibit akt and erk activation respectively therefore our results suggest that the activation of ras and its downstream molecules akt and erk to an appropriate level is one of crucial elements in the determination of osteoblast cell responses the ras pathway may represent a cell biological target that should be considered for successful surface modification of biomaterials to induce adequate cell responses in the bone tissue | MEDAL |
more than half of the maternal deaths worldwide occur in SSA most commonly during childbirth or the immediate PP period although delivery in health care facilities can avert maternal deaths many women in subsaharan africa continue to deliver at home AF influencing mothers decisions to use facilitybased delivery services in rural lowincome settings are not well understood health care professionals who provide delivery services in these areas may have unique insights about AF TPS to such settings accordingly we conducted a qualitative study of HCPs in rural ethiopia to determine key factors influencing facility delivery using indepth interviews and the constant comparative method of data analysis results suggest multiple influences on womens decisions to deliver at home including inadequate resources in facilities unappealing aspects of delivery in facility settings and known barriers to accessing services such as distance transportation and cost our findings suggest that local HCPs offer valuable insight into why many rural ethiopian women deliver their babies at home despite L1 efforts to promote facilitybased delivery their perspectives underscore the importance of a patientcentred RPA to delivery services which is often lacking in lowresource settings but may be fundamental to encouraging facilitybased deliveries | MEDAL |
plateletderived growth factor pdgf is a potent mitogenic and chemotactic protein for a variety of cell types glomerular mesangial cells also respond to pdgf in terms of proliferation but to date have not been examined for migratory behavior in response to a TPS growth factor here we examine the ability of isolated rat mesangial cells to migrate toward gradients of purified pdgf CTX assays were performed in twocompartment blind well chambers each compartment separated by a microns porous filter membrane human pdgf was added to microliters of rpmi medium in the lower compartment beneath the filters to make incremental concentrations from to unitsml control compartments received diluent without pdgf mesangial cells in rpmi medium were added to the upper compartments and the chambers were incubated for hours at degrees c after fixation the number of cells on the underside of the filter were counted by scanning electron microscopy a linear dose response of mesangial cell migration toward increasing concentrations of pdgf was observed achieving cell numbers of fold over controls at unitsml migratory cells were verified as mesangial cells by fluorescence expression of actin S1 and desmin and absence of expression of leukocyte OEP and ia antigen addition of equimolar concentrations of pdgf on both sides of the filter or addition of antipdgf antibody to the lower chamber containing pdgf negated the chemotactic response these studies indicate that mesangial cells migrate in response to pdgf this mechanism may in part play a role in some forms of mesangial proliferative glomerular disease | MEDAL |
direct compression tablets containing sodium ST glycolate an alginate derivative or povidone as a disintegrant magnesium stearate as a lubricant amaranth as a tracer and dibasic calcium phosphate dihydrate as the matrix were stored for days at degrees and relative humidity rh degrees and rh and degrees and rh samples were evaluated T3 and days for size hardness and dissolution characteristics although no significant changes in the dimensions or hardness of the three tablet formulations prepared at three different compaction pressures were observed the DE of the systems showed significant changes some systems dissolving more rapidly and some more slowly T3 storage in some cases the changes were so substantial as to indicate the possibility of significant changes of the bioavailability of drugs formulated in such systems the relevance of this work to the problem of evaluating aging effects on the PCS properties of tablets is discussed | MEDAL |
measurement of the catalytic activity concentration of enzymes has been standardized using a traceability chain consisting a REF measurement system for enzyme catalytic activity and REF standardjscc enzyme the japan medical association jma has provided an external quality assessment eqa survey program for clinical laboratory testing more than clinical laboratories participated in the eqa program indicated that standardization of the measurement of the catalytic activity concentration of enzymes has been completed for ast alt ld alp gammagt and ck in more than laboratories and for amy and che in nearly of laboratories because such a C1 survey program must use artificial specimens a matrix effect cannot be avoided especially in dry chemistry however the bias produced by a matrix effect usually has a predictable tendency it can be QTc next after standardization of the measurement of the catalytic activity concentration of enzymes we should develop and use common REF intervals on completing the standardization we can make standard medical decisions using REF measurement systems and rules | MEDAL |
patients with cancerassociated thrombosis are at a high risk of developing recurrent events despite anticoagulant therapy escalation of the dose of low molecular weight heparin lmwh has been suggested as a potential treatment option to manage these patients we sought to confirm the benefit and risk of this management strategy in patients with recurrent cancerassociated thrombosis | MEDAL |
the purpose of this review is to summarize the effects of calcium NI on peak bone mass pbm accrual by premenopausal females during their s and possibly s prospective studies are highlighted because of the value of investigating the same subjects for one or more years results of crosssectional studies are also summarized findings from both two | MEDAL |
pancreaspreserving total duodenectomy pptd was first described by chung et al in since then several surgeons have used pptd to treat diseases that involve the duodenum diffusely but not the head of the pancreas mostly familial adenomatous polyposis fap the pptd method has been changed in each report and seems to have improved over time we performed pptd on three patients with different diseasesone with intestinal hemorrhage due to small intestinal amyloidosis another with numerous duodenal gastrinomas in a patient with MEN type men and ZE syndrome zes and the third with numerous DU polyposis and fapusing a new method that is simpler and safer than those previously reported when resecting the whole duodenum we performed mucosectomy of the major papillar portion and saved the structure of the L1 papilla after an approximately mm long sphincteropapillotomy the opened L1 papilla was anastomosed to an incisional opening of the small intestine the orifice of the main PD mpd was stented by a catheter and the mpd was kept intact under direct vision during the operative procedures the head of the pancreas was fixed with the small intestine by interrupted silk sutures reconstruction of the alimentary tract was performed T3 either the Bi or the billroth ii method this is the first report of pptd in which the entire mpd was preserved to simplify the biliopancreaticductal reconstruction | MEDAL |
arachidonoylglycerol ag is an important endogenous signaling lipid that activates the cannabinoid receptors B6 | MEDAL |
this is a prospective study using noninvasive electrical cardiometry to measure hemodynamic changes during surgical ligation of patent ductus arteriosus pda in very low birth weight vlbw ⤠g infants the aims of this study were to examine hemodynamic aberration caused by abrupt closure of a ductal shunting and to define AF that affect hemodynamic changes simultaneous measurements of heart rate hr stroke volume sv cardiac SO co and systemic V1 resistance svr were collected at ten time points h prior to anesthesia at the beginning of anesthesia starting of surgery immediately after pda being ligated and h followed by and h after the surgery thirty infants with gestational age of ± weeks and birth weight of ± g were studied upon sudden termination of ductal shunting there was a significant decline in co to of presurgery baseline the deterioration in co was associated with a decreased sv rather than hr at the same time there was an increase of svr following ductal ligation magnitude of co and sv reduction were higher in smaller infants ⤠kg and recovery was to a lesser degree in infants with more severe pda | MEDAL |
itch is an unpleasant sensation with the desire to scratch although it is well known that scratching itchy skin is pleasurable the cerebral mechanisms underlying this phenomenon are poorly understood we hypothesized that the reward system is associated with scratchinginduced pleasantness to investigate this hypothesis a PET magnetic resonance imaging study was performed in healthy subjects pleasantness was evoked by scratching the wrists where itch stimuli were applied while scratching the dorsal forearms far from itch stimuli did not evoke pleasantness interestingly pleasantness evoked by scratching activated not only the reward system ie the striatum and midbrain but also key regions of perception ie the primary SmI and awareness of subjective feelings ie the insular cortex indicating that a broad network is involved in scratchinginduced pleasantness moreover although itch was suppressed by scratching motorrelated regions such as the supplementary motor area premotor SC and cerebellum showed significant activation when pleasantness was evoked this activation could explain why scratchinginduced pleasantness potentially reinforces scratching behaviors this T0 is the first to identify networks activated by scratchinginduced pleasantness the results of the present study provide important information on the cerebral mechanisms underlying why scratching itchy skin evokes pleasurable feelings that reinforce scratching behaviors | MEDAL |
some gramnegative pathogens import host heme into the cytoplasm and utilize it as an iron source for their survival we report here that hmus encoded by the heme utilizing system hmu locus cleaves the protoporphyrin ring to PR iron from heme a liquid chromatographymass spectrometry analysis revealed that the Kd products of this reaction are two biliverdin isomers that result from transformation of a verdoheme intermediate this oxidative heme Kd by hmus required molecular oxygen and electrons supplied by either ascorbate or nadph electrons could not be directly transferred from nadph to heme instead ferredoxinnadp | MEDAL |
hemodilution reduces blood viscosity and o content cao and increases cerebral blood flow cbf viscosity and cao may contribute to increasing cbf after HD however because hematocrit is the major contributor to BV and cao it has been difficult to assess their relative importance by varying blood viscosity without changing cao prior investigation in hemodiluted animals has suggested that both AF play roughly equal roles to further investigate the relationship of hemodilution blood viscosity cao and cbf we took the opposite RPA in hemodiluted animals ie we varied cao without changing BV hyperbaric o was used to restore cao to normal after hemodilution pentobarbital sodiumanesthetized rats underwent isovolumic HD with hetastarch and forebrain cbf was measured with hnicotine one group of animals did not undergo hemodilution and served as controls con in the three exp CG hematocrit was reduced from to con and hemodiluted hdil groups were ventilated with o at kpa atmosphere absolute which resulted in cao values of and sd ml odl respectively a second group of hemodiluted animals hbar was ventilated with o at kpa atmospheres absolute in a hyperbaric chamber which restored cao to an estimated ml odl by increasing dissolved o a fourth group of hemodiluted animals hcon served as hyperbaric controls and were ventilated with o at kpa resulting in cao of ml odl cbf was ml g min in the con group and significantly increased to ml g min in the hdil group when cao was restored to baseline with dissolved o in the hbar group cbf decreased to ml g min when normoxia was maintained during hyperbaric SE in the hcon group cbf was ml g min a value indistinguishable from that in normobaric hdil animals our data demonstrate that the reduction in cao after HD is responsible for of the increase in cbf | MEDAL |
Subsets and Splits
No community queries yet
The top public SQL queries from the community will appear here once available.